LOCUS       DAEMHW010000001        63324 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEMHW010000001 DAEMHW010000000
VERSION     DAEMHW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 63324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.001"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(21..1571)
                     /locus_tag="KPK67_000001"
     CDS             complement(21..1571)
                     /locus_tag="KPK67_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG2457028.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(1665..1931)
                     /locus_tag="KPK67_000002"
     CDS             complement(1665..1931)
                     /locus_tag="KPK67_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBG2457029.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(2079..2939)
                     /gene="yabG"
                     /locus_tag="KPK67_000003"
     CDS             complement(2079..2939)
                     /gene="yabG"
                     /locus_tag="KPK67_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBG2457030.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(3023..3961)
                     /locus_tag="KPK67_000004"
     CDS             complement(3023..3961)
                     /locus_tag="KPK67_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBG2457031.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(4071..4232)
                     /locus_tag="KPK67_000005"
     CDS             complement(4071..4232)
                     /locus_tag="KPK67_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457032.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(4242..4739)
                     /locus_tag="KPK67_000006"
     CDS             complement(4242..4739)
                     /locus_tag="KPK67_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG2457033.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(4854..5564)
                     /locus_tag="KPK67_000007"
     CDS             complement(4854..5564)
                     /locus_tag="KPK67_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBG2457034.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(5740..6270)
                     /locus_tag="KPK67_000008"
     CDS             complement(5740..6270)
                     /locus_tag="KPK67_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457035.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKPIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEIVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(6287..6517)
                     /locus_tag="KPK67_000009"
     CDS             complement(6287..6517)
                     /locus_tag="KPK67_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457036.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(6532..7926)
                     /locus_tag="KPK67_000010"
     CDS             complement(6532..7926)
                     /locus_tag="KPK67_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG2457037.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLIKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(8052..9305)
                     /locus_tag="KPK67_000011"
     CDS             complement(8052..9305)
                     /locus_tag="KPK67_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2457038.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(9356..10510)
                     /locus_tag="KPK67_000012"
     CDS             complement(9356..10510)
                     /locus_tag="KPK67_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2457039.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(11070..11639)
                     /locus_tag="KPK67_000013"
     CDS             complement(11070..11639)
                     /locus_tag="KPK67_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2457040.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            11842..12069
                     /locus_tag="KPK67_000014"
     CDS             11842..12069
                     /locus_tag="KPK67_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2457041.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(12288..13031)
                     /locus_tag="KPK67_000015"
     CDS             complement(12288..13031)
                     /locus_tag="KPK67_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG2457042.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            13199..14071
                     /locus_tag="KPK67_000016"
     CDS             13199..14071
                     /locus_tag="KPK67_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2457043.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            14071..14736
                     /locus_tag="KPK67_000017"
     CDS             14071..14736
                     /locus_tag="KPK67_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG2457044.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            14757..15614
                     /locus_tag="KPK67_000018"
     CDS             14757..15614
                     /locus_tag="KPK67_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2457045.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(15770..18316)
                     /locus_tag="KPK67_000019"
     CDS             complement(15770..18316)
                     /locus_tag="KPK67_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2457046.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(18316..19002)
                     /locus_tag="KPK67_000020"
     CDS             complement(18316..19002)
                     /locus_tag="KPK67_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2457047.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(19085..20074)
                     /locus_tag="KPK67_000021"
     CDS             complement(19085..20074)
                     /locus_tag="KPK67_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2457048.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(20061..20747)
                     /locus_tag="KPK67_000022"
     CDS             complement(20061..20747)
                     /locus_tag="KPK67_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2457049.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(21247..24453)
                     /gene="carB"
                     /locus_tag="KPK67_000023"
     CDS             complement(21247..24453)
                     /gene="carB"
                     /locus_tag="KPK67_000023"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG2457050.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(24646..25695)
                     /gene="carA"
                     /locus_tag="KPK67_000024"
     CDS             complement(24646..25695)
                     /gene="carA"
                     /locus_tag="KPK67_000024"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG2457051.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(25953..29159)
                     /gene="carB"
                     /locus_tag="KPK67_000025"
     CDS             complement(25953..29159)
                     /gene="carB"
                     /locus_tag="KPK67_000025"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG2457052.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLEYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(29212..30255)
                     /gene="carA"
                     /locus_tag="KPK67_000026"
     CDS             complement(29212..30255)
                     /gene="carA"
                     /locus_tag="KPK67_000026"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG2457053.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKRLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKKLPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(30281..30997)
                     /gene="pyrF"
                     /locus_tag="KPK67_000027"
     CDS             complement(30281..30997)
                     /gene="pyrF"
                     /locus_tag="KPK67_000027"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBG2457054.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMATEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVKEIENA"
     gene            complement(31373..32008)
                     /locus_tag="KPK67_000028"
     CDS             complement(31373..32008)
                     /locus_tag="KPK67_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBG2457055.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(32032..32946)
                     /locus_tag="KPK67_000029"
     CDS             complement(32032..32946)
                     /locus_tag="KPK67_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457056.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            33546..34775
                     /locus_tag="KPK67_000030"
     CDS             33546..34775
                     /locus_tag="KPK67_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG2457057.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            complement(34980..37361)
                     /locus_tag="KPK67_000031"
     CDS             complement(34980..37361)
                     /locus_tag="KPK67_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBG2457058.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(37333..38058)
                     /locus_tag="KPK67_000032"
     CDS             complement(37333..38058)
                     /locus_tag="KPK67_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBG2457059.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(38048..38503)
                     /locus_tag="KPK67_000033"
     CDS             complement(38048..38503)
                     /locus_tag="KPK67_000033"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBG2457060.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(38785..39966)
                     /locus_tag="KPK67_000034"
     CDS             complement(38785..39966)
                     /locus_tag="KPK67_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2457061.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSIISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(39956..40648)
                     /locus_tag="KPK67_000035"
     CDS             complement(39956..40648)
                     /locus_tag="KPK67_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2457062.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(40782..41498)
                     /locus_tag="KPK67_000036"
     CDS             complement(40782..41498)
                     /locus_tag="KPK67_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2457063.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AIEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            41623..42357
                     /locus_tag="KPK67_000037"
     CDS             41623..42357
                     /locus_tag="KPK67_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HBG2457064.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            complement(42642..43493)
                     /locus_tag="KPK67_000038"
     CDS             complement(42642..43493)
                     /locus_tag="KPK67_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2457065.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDTIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
     gene            complement(43519..44457)
                     /locus_tag="KPK67_000039"
     CDS             complement(43519..44457)
                     /locus_tag="KPK67_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG2457066.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            complement(44560..45210)
                     /locus_tag="KPK67_000040"
     CDS             complement(44560..45210)
                     /locus_tag="KPK67_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG2457067.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            complement(45359..45547)
                     /locus_tag="KPK67_000041"
     CDS             complement(45359..45547)
                     /locus_tag="KPK67_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG2457068.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            complement(45654..46838)
                     /locus_tag="KPK67_000042"
     CDS             complement(45654..46838)
                     /locus_tag="KPK67_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2457069.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            complement(46980..47897)
                     /locus_tag="KPK67_000043"
     CDS             complement(46980..47897)
                     /locus_tag="KPK67_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBG2457070.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            complement(47919..48647)
                     /locus_tag="KPK67_000044"
     CDS             complement(47919..48647)
                     /locus_tag="KPK67_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2457071.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            complement(48843..49043)
                     /locus_tag="KPK67_000045"
     CDS             complement(48843..49043)
                     /locus_tag="KPK67_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457072.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            complement(49168..49548)
                     /locus_tag="KPK67_000046"
     CDS             complement(49168..49548)
                     /locus_tag="KPK67_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2457073.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            complement(49821..51179)
                     /locus_tag="KPK67_000047"
     CDS             complement(49821..51179)
                     /locus_tag="KPK67_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2457074.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            51627..52079
                     /locus_tag="KPK67_000048"
     CDS             51627..52079
                     /locus_tag="KPK67_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBG2457075.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(52236..52853)
                     /locus_tag="KPK67_000049"
     CDS             complement(52236..52853)
                     /locus_tag="KPK67_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBG2457076.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFIYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(53275..54087)
                     /locus_tag="KPK67_000050"
     CDS             complement(53275..54087)
                     /locus_tag="KPK67_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2457077.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNIFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRIL"
     gene            complement(54163..55581)
                     /locus_tag="KPK67_000051"
     CDS             complement(54163..55581)
                     /locus_tag="KPK67_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBG2457078.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            complement(56065..56982)
                     /locus_tag="KPK67_000052"
     CDS             complement(56065..56982)
                     /locus_tag="KPK67_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBG2457079.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIETVKEFWE"
     gene            57244..57621
                     /locus_tag="KPK67_000053"
     CDS             57244..57621
                     /locus_tag="KPK67_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2457080.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            complement(57777..58226)
                     /locus_tag="KPK67_000054"
     CDS             complement(57777..58226)
                     /locus_tag="KPK67_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2457081.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(58390..58794)
                     /locus_tag="KPK67_000055"
     CDS             complement(58390..58794)
                     /locus_tag="KPK67_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBG2457082.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            complement(58907..59773)
                     /locus_tag="KPK67_000056"
     CDS             complement(58907..59773)
                     /locus_tag="KPK67_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG2457083.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSSELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            complement(60343..60687)
                     /locus_tag="KPK67_000057"
     CDS             complement(60343..60687)
                     /locus_tag="KPK67_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBG2457084.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            complement(60876..61493)
                     /locus_tag="KPK67_000058"
     CDS             complement(60876..61493)
                     /locus_tag="KPK67_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2457085.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            complement(61498..>63324)
                     /locus_tag="KPK67_000059"
     CDS             complement(61498..>63324)
                     /locus_tag="KPK67_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBG2457086.1"
                     /translation="NTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNE
                     DTINKILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISV
                     SREASILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFA
                     MFSIICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILS
                     INNCILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNL
                     VEEKDAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNG
                     KEIFNLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNK
                     TKKSDLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRC
                     VYFNSKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIII
                     AISNLMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTR
                     VDLSNNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
BASE COUNT        20447 a        11218 c         6928 g        24731 t
ORIGIN      
        1 ttactttaaa ttttttaata ttaatctact aatacgactt ttttttctaa aataagacac
       61 ttaccaggct taattggttc atcaagtttt atatcattaa gttctgcaat ttcattttct
      121 gttgtattgt atttttttgc tatattccag aaagtatctc cttctttgca tatatatacg
      181 attatgctag gagctttaga taaatcatat acaccttggt cttctccctt gataataaaa
      241 ttttctgctt tcttatctaa tgcttcagta aatctcttaa ttttgataat caagtctatc
      301 tggtccctat tcaaatcaac ttcgacttta tcaatacatg ctgtattaaa cacagatgct
      361 gtatcagtta gattatccat ttctatatca tgttcaaatg gaatttcctc actaatttta
      421 taaaccaatt ttaaaccttc aactgggaca aataaaattt caacttttat gattccttgt
      481 ataatgcttt tatttccttc aatataacta ttttctatag acatggttgg acaaacgctt
      541 acaatatctt ttatttgtat atcatcatta tcatttctta tgccttctct aaccataaat
      601 gtttcttcac tacgtcttaa agtcttattt aattgaatag gtttgtggtc aaattttatt
      661 atcttttgag gcgaatatgc atcttgtaat acttctcttg taacttcatc tgtaactttt
      721 accttacaac aagccataca atctatttct aaaagacctg tgttgctttc actattttgc
      781 ttgaaaatat gattaaaatc tgacatagac aacaatactt cttcagtcat accatcacta
      841 actccaggga cttctacaaa ttgagtaaac tcaataccta ctctatccaa ctctacaagt
      901 tcaccctcat atgtacaagc taacggattt atttctaata ctccaccaat aataacttta
      961 ttgtcagtca ctctactttc ttttacttta acacatggat ttaaacttat tatagattga
     1021 atttcctctg tgtttattgt tatagtatct cttatagaac tttctgcttt ttctattcca
     1081 actatatctt gaaaacaaat ttcctttctg tgcttttgta tgccttccac ttgcgccaca
     1141 tcttttacaa tgtctaacct ttgcttttca aacaaacttc cccttatatt cataagagct
     1201 cctactttaa tctttctctc attcattata gtacaatcca tatgctctac ttctgaaaat
     1261 aacatgtatt ccatatcttg aataacattg tctttttcta ttacttcgtt tatatctact
     1321 tttccgtcta cattagaaat agtattttta tcatctgcaa tatagattac attataatta
     1381 aaactacctc tgcaaagtat cttcccatct gccatttcaa tttttttaag tgatatgtat
     1441 ccttctgttt ttacaatttc atatacatct aattttttgt ctggtacaac tgcctccgct
     1501 tcaatgaatg tttgaaattt tccaaagtct attctattgt caactttaat tacatcttta
     1561 attaattcca tagtctacct ccttaagtat tataaaaata tcacactata tttatattta
     1621 aggagcaaaa aaaaaattac acctattttt aaataggtat aattttaact tatttgtaat
     1681 ttatcttgat ctctaaaaac ttgcaacttt acattagatg ttaataaatc tgagtaacta
     1741 taagatactc taggatatcc attactttca tcatctaact ttataacaaa cacgcttgga
     1801 taaacttttt ctagtatacc ctcttttgta ataatctgct ttcttccttt attagctttc
     1861 aacaatattt tctttcctat atgtctctct aagcttactc ttatcttatc tagagtttga
     1921 acagtagcca caatatcacc ttctttaacg tttatatgtt tacataatat cacatataaa
     1981 catttttgtc aaatgtttaa actaattatt ttatatattt tttgcaaaaa tgtcaatact
     2041 atttttgaag atatacagta taattatgtt aatttttgct aatgtaatat tgtttttggc
     2101 attgcccatc taaattttcc tcttgtttca gaaccaccaa ttcccttaac acctgtaata
     2161 gtctgctcta taacctcatc aataggaact actacatcaa ttcttgaaca agcaattttc
     2221 tccactataa aaacagggtc taatgcatgt atcattattc ttccaggcga acttgcatag
     2281 tttgctcctg cacttattat tttttcataa tttgattggc atgctcctgc gaatatgaca
     2341 agattatcca aatttggttg ccatttacgt gcttctttta ctgttttaac aaaatttgaa
     2401 gagtttctat agttactcat atcttctata tctcctcttt ttattgtcat tgcatcatga
     2461 cctgttatta ctagtatatc cggattatat ttttctagta gtgctcttac ttctttatac
     2521 tgattttgct ctgaaacagc tacacctact gcaggtattc caagttgagc atatacatcc
     2581 aagcaaattt ttaaatattc tttatctcca tctatctgta gtacttttcc aggcattcca
     2641 tatgtgttag gatttaattg aagttttgga actgctctag tcattctttt ttgtctttct
     2701 ttagcttttc ttactgactt atataataaa ttttctacat ttttatctat taatatatct
     2761 tttatatcag gtgcttttac taattctagg tcatcaatat aagcatctgc tattactcta
     2821 aatgcaattc cctttagtat agctattttc tcattatttt catctacacc aaaagagact
     2881 attttaaata ctatatcttt gttatgtgac tttctagcta caatgtctcc taccttcata
     2941 gttaaacctc caataaattt ttatcttagt ttatactatg ttcgatataa aactttgttt
     3001 cctaatgtca aaaaaacatt tcttataata cttcttttat cactcttaaa gcatttttat
     3061 agtatatctt ttctatatca tcttcactaa acccttcttt ttttagaggt tcatacagtt
     3121 tacccatctg agatatatct tcaatctcaa cttttgaatc tattccatca aaatctgacc
     3181 ctagggatac aacatctatc cctccaacat ttacaatatg ttttatgtgt ctaaccatat
     3241 cttcaagttt actttcactt ttatcactta aaaataagtg gaagaagttt atacctgtaa
     3301 ctccaccttt atttgctaat actttaatca tatcatcagt taaatttcta gaatgattca
     3361 tcattgctct tgaattagaa tgtgtagcaa ttattggttt agaagaaatc tttgcaattt
     3421 cataaaatcc accatctgaa atatgagaaa catcaaccaa cattcccaat tcattcatct
     3481 tatgtaccac ttctcttcca aaatcagtaa gccctttttc tctatattct actttattat
     3541 gaggaaagct caattcattc acatgattcc aacttattgt catcattcta acccctaagt
     3601 cgtaaaattt ctttaaattt tctaatttac cttcaagtac tgcaccttcc tcaatagata
     3661 aaagagctgt aagtttacct tctgattggt ttttcataat ttcttcatag ttagtagcta
     3721 aagctatttc atcactattt tttttcattt cctcatgaaa tttatttgcc atactcatgc
     3781 aataatcaaa aggatgtttt acttcttcag tatcaacaaa aagagcaaat gtttgagcta
     3841 atgaatcccc ctttttcaat ctgtctatat caacagaata tttattagac ttaagttcac
     3901 ttgtttctac attttccatt aattttgcta tagtatcaca atgtaaatca ataaatttca
     3961 tatttatacc ccctgtatat tttatattta tatctaaaaa tagaaatagt tgtccttata
     4021 gacaactatt gacaactatt ttttaatatt ctttttaaca ttacttaatc ttattcttct
     4081 tttaagttag gtattacttt tttaacttct tcaatttgat gccatgtttt atctatatta
     4141 ttttcactaa tatatttttt taaatcagtt aaagatgtaa aatctttaac tgataaattg
     4201 tcatcatcat ctgcaaatct taaaataaac atgagactcc cttactttcc acaacatttc
     4261 ttatattttt ttccgcttcc acatgggcat gaatcatttc ttcctatttt tgcttcttta
     4321 actattgttt tagacttctt ataagccttt attatttctt ttctttggtc tgctggtagt
     4381 atatcttccc aaccttgtaa attatatagc caatgagcct caacagttac catattgtta
     4441 tataattttt cccaatctat ttttatagaa acttctttat cagcttctac ttcttctaaa
     4501 actatatctt cttttaagct ttcacttatt ccatctatga atcccatgaa aaattcatta
     4561 gtagttccaa atttttcagc caattcactt actttacctt caacaacatc cattttatta
     4621 tttaatattt cattgtatat tccagcttca actttcaaat attcttccca gaattttact
     4681 tctgcttctt gactttcatg attatcactt aaatttctcc attcagtata taaactcata
     4741 ctttttcctc ctaagtatac ctatattttt atagttttta tagtataaaa tcttttagtt
     4801 agattggaag tttcttcttt ttgattatac catattctat ttaaagttaa ctcttatatt
     4861 atatcataat taaacaattc ttttaatact tttatagcat actcttggtt tataaaataa
     4921 gggcttgtcg caattgttat tactttggct tttttaatta agttttttat cttgtaaaat
     4981 ttgagttcaa atggtatata gtccatatca cttgaaaata tatccaaatc tatgtcaaga
     5041 acaaactctg attctacttc taaatcaaat ccatatagac tatctattat tattaattct
     5101 gaaaatatgt tatattttaa agcaggtttt ataaaacttc ctacatttaa aacttcattt
     5161 gtatatctaa atacatcttt tagattattt acatctacat catagttctc aggttctcta
     5221 gtatccttat gttggtctac atgcactaac ttgcatcctt ttgtaaactc accctttaac
     5281 aaagattttg cccaaaaata aaatgcatgg ttatgattgt caaacacata tatcttttta
     5341 tttcccagga catattcgac catattctcc aatccttttg cttttatttc cttattttca
     5401 tctacttcat taaatactac ctcattgcct aaccttacat catttaatgt accatttata
     5461 agttttggaa cgtatatgct cttattttct cttttatcat atgaaaatac attatttcct
     5521 actggttttt ctatataaaa tccactatat tcataatttt ccataaaact ttatttcctc
     5581 cattgttata taaatatata agttataatt ttaaaaatta aaatgcactt attgtaaatc
     5641 atatcatcta ttatagaaaa catcttatat aactaaatat aatatcttct ctcaatatgt
     5701 attatacaat aagtgcaaaa tatatttata taaaatatac tacattccta taacaaaacc
     5761 atcagcttca attggaaggt aactaactct tctattcaac tccacctttc caaaaaatac
     5821 tacccaatat ggtctaaaaa tactttcaat tttaactatt tcaatctctg ggattcctcc
     5881 cataaaccta tgagttaatc tcatagccat tttatttgcc tttgctttta ctcttcgttc
     5941 gtccttatca ctatactgca caatattaca atccacattt tttacaattt catatgtagg
     6001 aaaatcttgt actatactac cagaacctgt agaaccattc actaaaattt ttaattcatt
     6061 tatatcatta gaatatctcc tattcttgaa aatgggtttc gttttgtgaa ttttaagtgt
     6121 gataatttta aactctatat aatgaagtct aacttcatga gcatatttgt tctttaatat
     6181 tagcttacta aataaagaat tttttccact tttagctatc tctatagctt cttcttttgt
     6241 aattcttata ggtaacgctc ttatatccat aaaatcattc tctttcttat ttttttcttg
     6301 tcatttttaa aactctatat atataatcaa aaaatattac aattacaact gccataactc
     6361 catatggaat tatttttcca actataatat catttctagt caatatacca taaaatataa
     6421 atcctgttat taaaaagtac aataatacta gaaaaccgtc tattccaagc cattttattt
     6481 ctaaatcttt ttttacctta gacactaatt tagacatagc agtttctcct tttatagatt
     6541 attttgtgta tttaaatttt cacctattct attaccaatc atccatgcaa taaatgaaaa
     6601 aggtatagct atcacaagag gctctaactc aaagctctga actggaaata ttttaaaata
     6661 accaaaagtc actccaccaa caatcattcc taaaatacca cccatagatg ttcttcttcc
     6721 atttccaaga agcccaccta ctataggtac aaatgccgaa cccatccaaa tacttcctac
     6781 aaatatacat gcatcatata cattttcaaa tctaaatgct acgctaagcc ccataactgc
     6841 tgctaaacat acacctattc tagtaaactt tattaattgt ttctgagata actctttttt
     6901 tgtaattctt ccatatatat catttgctaa tgtagctcca gctgctagat aatagctatc
     6961 caatgttgat attatagaac ctaaaattcc tattataaat attactctta ttccttgtgg
     7021 taatgtatct aaaactaaag ttatatatcc agcagctgga gccatttctg gataagtagc
     7081 tcttactata agtcctgtca tagttaacac catgtcaaaa catatccata gtgaaaaaca
     7141 agttaataag gctcttcttc caaccttagg agaatctgct gctgaaaatc tttgataaaa
     7201 agttgggtct gaataagctc caaaagaata tacaattaac atcaatgctt tcatagctga
     7261 catacctttt gtagggtgca taagctgtgg tgcatctttg agtgcttctg taagaccttg
     7321 aaaccctcct atttgttcaa attgacctgg cataaccata gttaaaccaa ataccataca
     7381 aaaaaacaat accatatctg taatggcaac tcccataagc ccaccaagta ctgtaagtac
     7441 aacgactact cctaccatta ttaatcctat taattcttta tctattttcc atgctacgtt
     7501 acctatagtt cccatagcag ttatttctcc aagttgtgca caatatataa acatcaaaat
     7561 tgccccaatt atagcaacat gcttgccata aaaactttcc agtaaatcag gaactgttat
     7621 attggttctt atatgaatcc ttggaccaac ccacaatgct aaaggcattc tcccaatatg
     7681 tgcagctatt gaccaaactg cccatgcaga tattccatat atagccgcat actctacact
     7741 acctactgta ccaactcctc cataccatga tgcaaccaaa gttcctacta ttaaggacca
     7801 tggtaaactt cgatttccta ggtaataact ttccatatct tttacatttc tagaaaaata
     7861 ataacctatt cctaataata taaatgcaaa tataactata atcccatttt cccaaaatgt
     7921 taacatatct aatctccaca ttcttatatt ttaattttta actttaatat ctaaatatga
     7981 acaactctac ccatttccat aaaaataggt agagttatat aataattata attatttaag
     8041 taatcttgtt attaagcaat tttattgttt aaacctttat tttctctttt atttatttta
     8101 tttaaagcat aagtagcaaa tatccctata actgcacata ctacagataa aataaatata
     8161 tatgtatatc ctgtttgtcc atatttatct aaccaattac ctgcaatagt gtaataaaac
     8221 gcatctggag aaaatcctat aaatgaagca aatccaacta ctgctcctgt agtttctaaa
     8281 gatatattag actctgaaac agatgcaaag tatatacctc taaatccaaa taaaattaca
     8341 cttagtatta tcatgtttat tacagctatc catataaatc ctgtactctt aggaataacc
     8401 aagtaagcag ctgtacttac tgtcattaat ataaatccta caaacattac tcttatagat
     8461 gaaccaactt tatctgttat aacaccagca actggagatg ctcccatttt tataacataa
     8521 gttcttatta ttgataaagc agaaactaga gtcattggca ttacataaac ttctgataag
     8581 tatggactta aataagttaa acttgagaat actaaataag ttgaaaatat tataagacca
     8641 attaaccaag cctttggcat tgtaactact tttaacaatg acttaatact taatccttca
     8701 tctgatgctc cactatcttt tttctcttcc actaagaaaa atagaagtat accacatatt
     8761 atagatgctg cagaatataa ccatactaca tatttgaatc ctactgttat ttcagcaaat
     8821 ttaccaaaga aataaagacc tataaaagaa attaatgtac ctgatacacc tgataaccct
     8881 tcataaaaac caaatattct tccttgctct gactcacttc cttgcattct aacaacctta
     8941 acagaggctg agaagaaggt taatattgtc gtaaatcccc ataatacaaa tattaaaagt
     9001 atcatactat atgatgggaa tgtagagaaa taaaatccaa gtattcctga agcaatcaaa
     9061 gaaaaagtta ttaattttct aactgagaat ctatctgcaa accatcctcc tataaaataa
     9121 gataccattc ctactaatcc atataaactc aataagttcc ctatttgtgt atgacttaat
     9181 gctaaagctt gttgcattgg gtcataaaat gatgatttca tatatggtaa actatacata
     9241 gctgttgttc caaatgccaa tatgaatatt atcaaaaatc ttttaacatt atttttgctt
     9301 tccattgatt ctctctcctt aaaattttat tgtttataat ctcattcata taattttatt
     9361 ttaatttttc tattgcttgt ttaaaatcat ttattatatc atttgaagat tctaatccta
     9421 cagctattct tattagattt tccccattca tttgaactaa tgtatgctct tcccctaagc
     9481 ttgttgctat tgttgcaagc tctaaagaat tcattaatct tctactagct tggtctcttg
     9541 tcaatccatt gataccatcc ttaagtttaa atgaaactac acctccacct aaaccattca
     9601 tttgagtttt acaaagctca tattgaggat gagattttaa tgctggatac tttaccaatt
     9661 ccacattctc ttcttcctct aaaaattctg ctactgctaa tccattttca caatgctttt
     9721 tcatcctcat atctaacgtt ttcatacccc ttaaaatcat ccatgcactc attggtggta
     9781 aagatgctcc tgtaaaacaa cataaacttg gccatctaac taaatctatt aattcttttg
     9841 aacctgctac tgctccccct aaagtatctc catgtccatt tataaactta gtaagactat
     9901 gtattactaa atctgcacct aaatctattg gtctttgtac aattggtgtc ccaaaagtac
     9961 tatcaactat agctaagctt ttattttcat gagcaatttc tgctattgtt tttatatcta
    10021 ctaagtctaa tgatggattt aaaggacttt ctatataaac tattttagta tttggtctca
    10081 ttgcttcttt tacattttgt ggatttactg catctacaaa tgtaacttca actccaaatt
    10141 tagataacaa ctcagacatg aataatttag tgtgtgaaaa tattccatct gaactaacta
    10201 tatggtcacc actttttacc aatgctaata tagtagatgt tattgccccc atccctgatg
    10261 tactagatat ggcactttct gcattttcaa gcatagccaa cttagcttct aactctgtaa
    10321 gagtaggatt acactctctt gtatacacat atccccatgt catacacaac tcttcaaaat
    10381 gctctactgt ctttgcagca aatgttgctg tttgacatat tggtgtagcc aatgcacctg
    10441 ttgttgggtc tgcactatgt ccccaatgta ttatttttgt ctctaaatct tgattaatat
    10501 tgttactcat tttgatttcc tcccattttg acttatttat acaacgtttt ccctgccaaa
    10561 tttcatcaat ttagcttagt cttaatacat tttttcaagt atactttttt atttgttata
    10621 atttctaaaa atatgacact tattttgttt ttttacagtt ctaattataa actctatagt
    10681 aactatagag tcaatatgtt tttgatattt tttttacaat gtttaaaata gtcagtacaa
    10741 aaataggtat atcttagaat aactaaagtc atcctaagat atacctattt ttatatcaca
    10801 atataaataa ttacatataa gcattagttt ttttatcata aatagatttt tcaagattta
    10861 tcatcttttc ataaatatat tatagttaaa tattttaata ttacctaaca tctacaataa
    10921 aatatactct taaaataaaa ttatataatt aaaaatactc tataaaagct aaaaagtttt
    10981 tctattcgct acatctaaaa ataattagat agaggtataa atgtatttag tttatttata
    11041 cctctagtat tttataaatt atatctctac taaattgatt ttttagcacg aataagtgtg
    11101 ctcttggaga taccagtcat cttcacaacc tctctatatg gcttagaatt taataacccc
    11161 aatgcatatt gtatctgttg attgctgtat ttctttggcc taccatcttt aaagtccttt
    11221 ttctttttag caaattcttt tcctaattga gtacgttcta ttatcatatt tctctcaagt
    11281 tctgcaactg caagtaatgt agtcaaaaag aatttaccca ttgaagtatt ttctaaaagg
    11341 cctatattta aaatatgaac acttacttct ttagcaaata aaccttctat tagttgtatt
    11401 ccttctgatg tacttctagc tagtctatcc agcttagttg caacaagtaa gtctcccttt
    11461 tttagagatg atataatttt ttcaaattta ggcctgtttg tagtagtgcc tgtataagtt
    11521 tcctttatta ttttagcatc attatattta ttcaaaattt cttgctgttg ctgatacaaa
    11581 ctgttgttgt ctgtttgatt ttttgtactt acacggcaat atccatatat aataatcatc
    11641 acttcctaac ataaagattt tgtacagaag ttacccagaa gcttgttttt acttatcttt
    11701 atccatttta ctaaaacgat ttttgggtgt caatactgtt aacttttgac accataataa
    11761 ttcctttata atttgtttga ctatgtcaaa aattaccaat ataatgaaat taatgagttt
    11821 atacaaattg gaggaaataa tatgaatagt ctgttaaaag aagcaataga tgaattaaaa
    11881 aacatccatt gcggggaagt ttttttatta aaagaattgt tcaaaggcta tgaatggaac
    11941 aggttggaga tgaatatcag attgaaacta ggaaaacttt ttttgtatga agtggtaact
    12001 aatcccaatt taaatgtaga ggtcatcaat aaaaattctg cttatcagca aatgtataaa
    12061 aaaaaatagt gacttaaatg tatataatag tataatcttc ttattgattc aaaagttaaa
    12121 acttttaata caaaacaatc attctatttt aacatttatc aatatgtcta aatttatata
    12181 attaacacat acgaatagta ttgtttaaat cacattattt aagatagata aatcttaacc
    12241 ctactccagc ataaatacta aaatagggct tagaattatc tatattttta tcccataaaa
    12301 tgcttagctc ttcctcttga ttgaacatat atatctgggg aaatacaata gaagcgtaaa
    12361 tttataaaat ttccaaagtc agaaaacttc agttcaaact taggtatacc aaaactttca
    12421 ggtgctattt cgtcaacacc aaaatagatg ataattccat tatctgtaag ataaaatgct
    12481 tggtcttcag gtataactaa ctcaacatta gaataataca tttctctatt ttcatttatt
    12541 tgagatttaa tgtaatcagt aattatatta atataatcaa ctccatctct aaaaatgtca
    12601 ttaagtaaaa gttcttttcc agttgtcaaa tctatattgt aattattcaa tgtattgtat
    12661 ttaggcccat agttattagc aaaccccata aggtttaata tcatactcag tacataattt
    12721 ttattaaaag taactgcgta actggtagat acccaatagc tttgttttgg aagtgaattt
    12781 tgagaagctg cgttattata ttctcgttct tcttcctgta agccattttt aaaattattt
    12841 acagtcattc ttacagcatt gtttatgtta tttaaaataa ctgggtttac atacgtggat
    12901 tgatttctaa tattgtatat aacaggatac tttacatcat atgaaaaata cttacttctt
    12961 tcaaaatctt cattaaattg tacatataat atattatatg tacaggtatt tgtagattga
    13021 taattttcca ttaaaatcct ccccctttta taaaaaaatc agatacaata taatatgctt
    13081 aaaacagtaa taatgttaat ggtaaatgta aagaatagaa agtatctaaa acagcaaaaa
    13141 atgactaatt ttgtggtata atacatagga ttgagagtta ttttatagga ggaaaaatat
    13201 gttattatta gcagataaat ggaaagatta tgaacttata gatatgggaa atggagaaaa
    13261 attagaacgt tggggaagtt atgtacttag aagaccagac ccacaggttg tatggccaat
    13321 ggaatctgaa tgggctttat ggaaaaaccc tcatggacat tatcacagaa gcaataaagg
    13381 tggaggtcaa tgggaacaca aaaaaagata tccagaacaa tggactataa attataaaaa
    13441 cttaaagttt cacattaaac caactggctt taaacatact ggattattcc cagagcaagc
    13501 agctaactgg gattggatga tagagaaaat aaagaaagct aacagaccta taaaagtgtt
    13561 gaatcttttt gcatatacag gtggagctac tgtagcatgt gcatctgctg gtgcagaagt
    13621 atgtcatgtt gatgctgcaa aaggtatggt taattgggca aaagaaaata tagaattatc
    13681 aggtcttaaa aatcaaacag taagatttat agtggatgat gttgtaaagt ttgttgaaag
    13741 agaaataaga agaggtaaaa aatatgatgc aataataatg gaccctcctt cttatggaag
    13801 aggtcctaaa ggagaagtat ggcaaataga agaaaagtta tacagttttg tagatttatg
    13861 tatgggagtt ttatcagata accctttatt tttcttaatt aattcttata caacaggatt
    13921 ttctccaata gttcttgaaa atgtattgtc tacaacaata ggtaaaaaga caaatacagg
    13981 agatatatac ggtggggaat taggaatacc agcttccaga gatggtaaga tacttccttg
    14041 tggtatattt ggaagatggg agaaaaaata atgataaaag tcatatatga agataaccat
    14101 ttattagttg tagaaaaacc agttaacata ttgtctcaag gagataatac taatgataaa
    14161 gatatggtaa atcttttaaa agactatatt aaaaaaaagt ataacaaacc tggaaatgta
    14221 tatattggtt taatacatag attggataga ccagtaggtg gagttatggt gtttgcaaaa
    14281 acatcaaaag cagcatctag attgtctgag caggtaagaa gtaagacttt caaaaaaact
    14341 tatctagctg ttgttcatgg caagataaaa tctaactcag atatactaaa agatttttta
    14401 tataaaaata aaaaaactaa tatggtaact gtagtgaaaa aaagccataa agatgccaaa
    14461 gaagctgaac ttaactataa tgtattagat tttaaagaaa atctaagttt agttcaagtt
    14521 aatttaaaga ctggtcgttc tcatcaaata agagttcaat ttgcaagcag aaaacatcca
    14581 ttatacggag accaaagata tggcaaagaa ataaataaaa ttggacaaca aatatctctt
    14641 tggtccaata agatagaaat ttgccatcct actacaaaag aaaaaatgga atttacatgt
    14701 catccacctg acaaatatcc atggaatata ttttaaatct caaatgaggt gattatatga
    14761 gtaaaaaatt ttcaatagga gaaatgtcta aacttcataa aataagcata cagacactaa
    14821 ggtactatga ccaaatagat ttatttaaac ctgttcatgt tgaccaagaa agcaattaca
    14881 gatattactc aattgaccaa ttttcacaat tagatattat aaaatattta aaatatttag
    14941 gtatgccttt aaaagaaatt aagtcaaagc ttgatgaaaa taatgaagaa attctaaaat
    15001 tactaacaaa taaaattaat atagtggatg aaaaaattaa agaattagag cttataaaga
    15061 aggtactaag cacaaagaaa gaaactatgg aaaaaggtat gaaggtagat gaagttggta
    15121 aaatagtgag aaaacatatt ccacgaagaa gtatcttatc tatagagtat gaagaaggat
    15181 taagtagtga agaaactatg gaactttcca gaagacaaat agcaaatata ttagaagata
    15241 atatatctgt gttctatggt ggagtaagtg gacttgtatc tatagataaa tttttaaaag
    15301 aagataaagt tatttatgac aatagttttg ttactgttga aagagactta tttgatataa
    15361 aatcacaaaa acatataaca gaaatttcat ctggtgattt tatttgtatg acatataaag
    15421 ggccttacaa agaaaattat accgctttta agaaactggt tgattatgtt gaatataaaa
    15481 gaatacctat tgaaaattat atatatgaaa tacctataat agaccctctg tctacaaaca
    15541 acgaagagga acttctaaca gaaattcaaa tacaagttaa aaagagagaa attgacttaa
    15601 gatgtctagt gtagtaatct agtttactta cacatcttaa tatagttgtc tattatcaac
    15661 atatgatttt tctagatagt gattcctaac agtgagattt tcaaaaaata ggaggaaata
    15721 tggctaatgc taattccctc ctatttttaa ttaatccata tcattttaat taagccatgt
    15781 catcacgaag tgcatcaata atattttctt tttttatctt attaatagta tacaacattg
    15841 taataaatac tataaataaa acactaaata tactaattcc aatactaaac catggaatca
    15901 caaaatcaat atcatctgta accattactt tatgaattag ccatgaagaa attactgcta
    15961 taggaagtcc aaagaacaat gctctcatgc cataaaaggc acactcaaaa ttcatcatct
    16021 tttggaaatc atgctcagac attcctacag agcgaagcat ggcaagttct ctacgacgta
    16081 acttaatatt agttgaaatt gtattaaaca cattggcaat cgaaatcaga gaaatcataa
    16141 ttatgaaagt ataggcaaaa acattggcaa taaaaatgta attacggttc tcatccatca
    16201 ttccatacat attgtacaga ttgtactcat tcgtaactcc ctcactctca ataatttgct
    16261 ccatttctgc aactgactga gtaggatttt tcgattgaaa gcttaaactt tcaattcctg
    16321 tagaaacacc aggaatctca aacttatctt tggctgaata aggagccaat actctgaagc
    16381 ttgctttctt cacatctgaa cttctgattc ctggaggaga atcaggtggc actactttga
    16441 caaatgaaat attcacattt tgtctctcag gtatctccaa cttgccatta gtttcaggag
    16501 tgaggctaac attcatagaa gaactgttga aaatatcaat aatattatta tcctttgtta
    16561 ccatagaaac agcaatcagt tttgcatttt gtccagtata ttcttctgct ggtaatccaa
    16621 gacttttaat aatattcaga taagttctat catcaagaaa ttgcatatct actggtaatt
    16681 caagcatttc actcatagaa tgtgaaccct catagttcaa ataatcgtct gtaagctcac
    16741 ttactttggc aacacatgaa taggtcataa gtttctgata caaaccttcg taaacaccat
    16801 ttgcagtttt tagcttatcg taaagcttca acatttcgtt gtctttcata ccttgtgtag
    16861 aaaaaataat atcctggtca gtaacctctg acctctgatt tgacatctgt tttaggtctg
    16921 ctactaaagc acttgtagat ataaatagaa ctatgcttaa aacaagcgac aatacaatac
    16981 ttcgataacg tttcttgttt cttttaaagt ttttcatagc aagaactccc tcaagaccat
    17041 aaactcgctg tgttagtttt gatgtcttca cagatttaga ttcaattttt acctcatttg
    17101 tctgacgaat acactccatc actggtgtgt tggcagcctt tcttgctggt atgtaagcag
    17161 aaattaagat tgtaaccata cttaccactg ctgcaacgat gattgcaggg atagacaatg
    17221 ttaatgtcaa cggaacgttt acatagagaa tatttgcaaa gtttttagct atgacagaaa
    17281 tcacaagtcc aatactgact ataccgattg caacaccaat tggtatacca atcgcaccaa
    17341 tgcaaattcc ttcaaatagc accgaatttc gtaactgttt tgctgtagct ccaacagatg
    17401 aaagtattcc aaattgatgt gttcgctcgt tcaatgacat gttaaatgaa ttatatatta
    17461 gaaaaattga gcctagcatg attatggcaa gcacaatgcc accaaccgag tacaaaaaca
    17521 tattgaacaa attgttatca gaaacaccta tgaagcgcaa cacattgtca ttgaaaatgg
    17581 aagcatggtc tttagtgcta cttgtgtaag agcgaatctg atgtggattt ttcaatgtga
    17641 caaaaaggct aagactgtca gtcttgactt gtttatcttc tttggttatc aaggtatatc
    17701 ctggtgcaga agattcttca aatacgggct ttctacagat acccacaact gtataagttt
    17761 ttttatcctg tggcacaaaa acttcactcc cagcagtgta tgggtcactc tgaccaagct
    17821 tttcattttc actcattcgt ttacctacat ccaaagaaat agtatcacct actgcaaatt
    17881 tgacaccacc atctgtcgca actttacttg aaacaagaat ctctctacta ttttcaggta
    17941 atcttccaga aattaaagtt ataggcaaag tatcaaaagc ttctttgcta aatccagcaa
    18001 taaaaagata tggtttatct ggatttctgt taccttcaag tgttgcatag ccaatatttt
    18061 caaatgttgc agtatttgca acttcatcat tgactctttg ttcctgtaca aaagaagaat
    18121 ccacatccaa aaactggaca tgccaatcac catattttat aatagctcca tttgtcatat
    18181 agttcatcaa agaaactcca aaggtagtaa ctgctgtaat catagcagct gacagaacaa
    18241 ctcctataat tgttacaaga gttcgagtgc gattcttttt caatccttgc agagcaactt
    18301 tgttaaaaat gttcattaac gattcacctt cctctcatca cgcactactt ttccatctga
    18361 aatacagata attctatctg cctgtaaagc aatgttttca tcatgtgtga caataatgag
    18421 agtttggtga tatttactat ggctctcttt cagtaactgg ataatctctt gtccattttt
    18481 actgtctaaa ctacctgtag gctcatcagc aagcataact gctggtgcat tcatcaatgc
    18541 acgaccaatg gcaacacgtt gctgttggcc accagaaagt tgatttggta aatgtgtttt
    18601 tcgttctttt aaaccaagta attctaacaa gtcatttaac cgttcttgat tgaccttacg
    18661 cttatccata agtataggca atgtgatatt ttccactaca tttaatgtgg gtatcagatt
    18721 gtgaaactga taaatcaatc caacttgtcg cctacgaaaa atagcaagct tttcattact
    18781 ttggccatat acatcttgcc cctccagata tatctttcca cttgttggta catctactcc
    18841 tgcaatggaa tgtagtagtg tagatttacc agagccagaa gaaccaatga ttgcagtaaa
    18901 ctcccctttt tcaatagtaa gtgagacatg gtcaagtgca gtaaccttat tttcattctt
    18961 accataaacc ttacataaat cttcaatctt taaaaacgcc attatataag tctcctttca
    19021 tattttataa atatatcata gaactttcaa cttacattta agtgacattt aaatgagaga
    19081 ttcgtcactt tggaaaacga ataacaaata ttgcaccacc ttgtgggtga ttttgtgcag
    19141 taattgttcc tccttgtctc ataataatcg tcttacaaag tgccaatcct attccataac
    19201 cagctgtgct tgaatttttc ccacgataaa atctgttaaa tagacatggt aagtcttctt
    19261 ttttaaagcc tgctccgttg tcatgaatag taagttgagt aaacaaaaag ttgtcctcac
    19321 aaatgatatc aatttttcca ttatcaccca cactctccat gcagttttta aatatatttt
    19381 gaattgcttc tgaaagccaa tttaaatcac ccaaaatcct tactcctttt ggtatatcaa
    19441 tatgtaaagt gatattgtgc agttccatgg gaatcaaaag ttgatgaagt gcagaactaa
    19501 tcaaattatt cacatcaatt tgttcttttt gaaagactac aatacctgca tctaaacgtg
    19561 ataattttaa aagggaagta atcagccagt ccattcgtat aagtaattcc tctgtttctc
    19621 gtatcaactc ttttctctca tcttcatcag aagtattctc taacagtgac aaaataagag
    19681 ttactgatgt aagaggagtg cgaagctggt gggctatgtc agctagtgag tcagcaaggt
    19741 gttctttttc ttttttcagt gcgtaatttt gctctctaat acgtaacgtc attttagtta
    19801 tttcactttg tagaatagaa agctcaccct catctgattc agcaatatac agatggttag
    19861 cattatgaag cacaagatta atttgttctg aaatctgtgc aatacttttg tatcgatttt
    19921 tagtaaacac aaaaaatgct attccaaagg tagtggcaga aacaatagaa agtattccag
    19981 tcataatact gattgcaaat cccagggcta cagtgagaat agtaattaat aagaataaaa
    20041 tagcaaattt tcgaaactct ctatttcgaa acataacttc cccccaatct ataccctgtc
    20101 ccacgaacag tcaaaattat ttgtggactt gaagggtcat cttctatctt ctctcgcaag
    20161 cgtttgatgt acacagtcaa tgtgttgtca ttaacaaact cacctgccgc atcccacaat
    20221 tcatcaagta atcttaccct cgtaataata cttttaggat tgctaataaa caccaataac
    20281 aaacggtatt ctaaagctga aagaaaaacc tcattgccat tctttttcac aacaccggtt
    20341 gctgtatcta cataaagccc acaaatttca aaagttgacc caaaacgccc actttttcgt
    20401 agtgctgttc taattcgcgc aatcagttca cgtggacgaa aaggtttagt aatataatca
    20461 tctgcaccca tattcagtcc agtaacaaca cttgattcat caccggaagc tgtcaaaaag
    20521 ataacaggaa tgtcttgctt ttctttgatt tctgtgcaaa tcgtaaaccc atttccatca
    20581 ggtaaagaaa tatcaataag tgccaaatca aatttattct tagcaatctc tacaagtgct
    20641 tcacttcttg tggatacatg ggttactata aatccttctg aattaagcaa aagaataaga
    20701 tttttggcaa ttgctttatc atcctcaact aaaaatatcc gtttcattta ctttcatcct
    20761 cctttatttt attagtttta tatattccag cagagcaata gtacactact attttactta
    20821 tcattacaca attttcagta gtttcttttg ttcttatata atccatgcat aatacctcca
    20881 ttgatttatt atatttctta taacataaat acacaagata agacaaaaaa cataatgtaa
    20941 gtgcaagttt tttaactcac taacttataa acatacactc ctactaacac tgctttcttg
    21001 atgacacatc ttacaagctt atattcatac atcaatgaaa aattaaattt atccatttat
    21061 taaaataaaa ggcaatagct tctatagcta atgcccttta tcttaaaatg atttaaatac
    21121 tatacaattt ttataaatgg ctataaataa actcgtatag aatataaaat ttatctacag
    21181 tcttttgtta tacaaaattt ataacctctt atagaacaaa tttaccactt tgcaaactta
    21241 tcacttttac tcagctatat tataaacttt taatttatct ttgtttaaat gtttcttctt
    21301 aacttctact aaagctttta gcgtatccaa agatgtcatt atttcagtag aaaattcaat
    21361 ggctgttctt ctcatcttaa atccatctct tgtagagtca tttcctttag ttggtgtatt
    21421 tattactata tcaacttgtt tatttcttat agcatctaat atgtttggtc taggttcttc
    21481 aattctattt actatatcag cctctattcc attttctctt aaactcttcg ctgttccttc
    21541 tgtagcaaca aaagtatatc ctaattcttt catatctttt gctatctcta taaactcatc
    21601 tttatcatga ttatttacag tagcaagtac tactcctctt tcatcagaca tatgtctacc
    21661 tgctgccaag aaccctttat ataaggcttc ttctaaattt tctcctacac ctagaacttc
    21721 tcctgtagac ttcatttcag gtcctagact tacttcaact ttagataatt tagacattga
    21781 gaatactggt acttttactg atactaattt tggctctttg taaactcctg taccatatcc
    21841 taaatccttt aatttagctc ctaacataca cttagtagcc aagtcaacta ttggtactcc
    21901 acttacttta cttatatatg gaactgttct actagctcta gggttaacct caattatata
    21961 taattcattt tgaaattcta taaattgtat attaaccata ccaagcacat taaggtctaa
    22021 agctattttt ttagtgtatt ctactatttt ttcttttatt tcatcagata tattttgact
    22081 tggatacatt gttgtactat ctccagagtg aactccagct ctttctaaat gctccattat
    22141 accaggtatc aatatatctt ccttatcaca tatagcatct acttctattt ctctaccagt
    22201 tagatattta tctataagta ctggattttt atggtctcta tcaaatgcat catccaaata
    22261 ctgaattagt ttttcttcgt tataagttat ttccatacct tgaccaccaa gtacataaga
    22321 tggtctaact aatactggat aacctaactc cttagcaatt tcaacacctt cattagttgt
    22381 ccatacacct tttccttttg gtctatttat atctagtctc tctagtaaat catcaaattt
    22441 ttctctatct tctgctgcat ctatatctct aaagtctgtt cccagtatag gtatattttt
    22501 ttcatgtaaa aacttagcca attttatagc tgtttgtcca ccaaactgta gtattacacc
    22561 ttctggtttt tctttttcta ttatatttaa aacttcttct tctgttaatg gttcaaaata
    22621 caacttatca gatgtatcaa aatccgtact tacagtttca gggttattgt ttacaattat
    22681 agtttctatt cccatttttc ttaaagattt tacacagtga actgaacaat agtcaaattc
    22741 tataccttga cctattctta ttggaccaga acccaatact acaacttttc tcttatcact
    22801 aactactacc tcatcatatt gttcataagt tgaatagtaa taaggtgata gagcttcaaa
    22861 ttctcctcca catgtatcaa ccattttata aactggttgt atattatata aacttctcaa
    22921 ttcatataat ttttctgggc ttatcttcat taagtcagat atacctttat cagaaaatcc
    22981 ctttttcttt aattcatgta aatattcttt atctaagtct tctattttta gaacttttaa
    23041 cttttcttct tgttctacta tccatttaaa tttatttata aaccacttat caacaccagt
    23101 tatttctgat atcatttcta ctttatagcc tcttcttatc atttcagcta agtcaaatag
    23161 tctctcatca tcaggtacta ctactctagc ctttaattct tctaatgttc taccttctga
    23221 tggagcatga accaatgaat attttcctgt ttcaagtgat cttatacctt taagaatagc
    23281 agcctcaaag ttacttccta tactcatgat ttcaccagtt gccatcatct tagttcctaa
    23341 ttttctattg gcatgtctaa atttatcaaa tggccacttt ggtattttta ctaccacata
    23401 atctaatgtt ggctcaaaac aagcataagt ttttttagtt actgcatttt ctatctcatc
    23461 caaagtataa cctaaagcta ttttagctgc tacttttgct attgggtatc ctgtagcttt
    23521 tgatgctagg gctgatgacc tactaactct tgggtttatt tctattatcg catattctaa
    23581 actatgtggg tttaaagcta tttgaacgtt acatccacct tgaacctcta ttgcatttat
    23641 tatgtctatt gatgcctttc taagtagttg atactcttca tcacttagag tttgacttgg
    23701 agcaacaact atactgtctc ccgtatgaac tccaactggg tctacatttt ccatattaca
    23761 tacagttata cagtttccat ttccatctct cattacttca tattctattt ctttccaacc
    23821 ttttatactc ttttcaagaa gaacttgtcc aacaggactt agctgtagtc catgtgaaag
    23881 tatttctctt aattcttcct cagtatcagc tatacctcca ccagttccac ctagcgtata
    23941 tgctggtctt acaactactg gatatcctat cttattggca aactcaagac ctgcttctaa
    24001 atttgtaact atatcactaa ctattactgg ttgatttatt tctttcatta cttctctaaa
    24061 taaatctcta tcttcgccct tttttataga ttctatagat gttcctataa cttttacatt
    24121 atatttatct aatataccag catcatgtaa ttctacagct aagtttaatc ctgtctgacc
    24181 acccatacct gcaagtaaac tgtctggtct ttctttttct attatttttt ctataaattc
    24241 tattgttaat ggttctatat atattttatc tgcaatttct ttatcagtca ttatagtagc
    24301 tgggttacta tttactaata caacttcaat tccctcttct tttaatgctt ggcaggcctg
    24361 tgttcctgaa tagtcaaact ctgcagcttg acctattatt attggccctg accctaatac
    24421 taaagttttc tttatactat ctaatttagg cataactctt tctccttatc taaacaattt
    24481 tcacagttct tatcttcatc atctatacaa tcaactttaa ttactttatt ttatcttaag
    24541 ttcaaattta ctcatcaata catctattta atacatctat gttaattaaa ccaaactttt
    24601 tgatttaagt tattcttgtt ttttagttag ctattctaat gttatttatt ttactaattc
    24661 taaaaattta tcaaaaatat agtcattatc ttttggtcct gggcaagctt caggatggta
    24721 ttgaacactg tatacatcta gcttctcatg tctcattcct tcaacagtat tatcatttaa
    24781 atttatatgt gtcactttca tttgttcagg aacttctgaa acataataac catgattttg
    24841 agaagttata aatacttttc cttcttctaa gtctttaact ggatgatttc cacctctatg
    24901 cccaaattta agtttagcag tttttcctcc aagagctaaa gctaaaagtt gatgtcctaa
    24961 gcatattcca actataggtt ttttaccaat aagagctttt atattttcta taacatcttc
    25021 taggtcttct gggtctccag gtccatttga caagaatatt aaatcagggt ttatactcaa
    25081 tacatcttct ggagatgttg tagctggaaa tattgttata tcacatccac gagctataaa
    25141 tgatcttaat atatttcttt ttactccaaa atccataaca gccacttttg gaccatttcc
    25201 ttttatatgc tctacttctt ttcttgtaac agttctaact gcttctgtat ttgaaaactt
    25261 atccaattta tgtttaacat cttctaattt actgtcttct aatgtaatta tacctttcat
    25321 tgttccatta tttctaagta tcttagtcaa agctcttgta tctattcctt caagtccgat
    25381 aactttattt tgctttaaat attggtctat atcaatttca catctaaaat tacttggaga
    25441 atcacttttc tcccttacta tgaatccctt tacttgaact ttttcagact ctgctgatga
    25501 taaatttata ccataatttc ctacaagagg ataagtcata gtaactattt gaccatagta
    25561 agaagggtct gttaaaactt caccatatcc tatcatcgaa gtattaaata ctacttctcc
    25621 tacactttct tctaaatatc caaatgcctt tcctataaaa actgttccat cttctaatat
    25681 taaccttgct ttcatactac agtacctcct ataattctct ttaggcaaac taaagaacat
    25741 taactaaata taagctttta aatatctata tctatttttc acttttacat tctagtttta
    25801 tacttattta catttatttt ttatcttttt ctattatact acaaatttct taaaaaaaag
    25861 aagttatctc aaaagtaaaa actcctagaa aaaataacct cttttttttt atttaataat
    25921 taaattctta aactatataa tcaacttaat ttctattcag ctatattgta aacttttaac
    25981 tcatctttat ttaaatgttt cttcttaact tctactaaag cttttagtgt atccagagat
    26041 gtcattattt cagtagaaaa ttcaatagct gttcttctta tcttaaatcc atctcttgta
    26101 gagtcatttc ctttagttgg tgtatttatt actatatcaa cttgtttatt tcttatagca
    26161 tctaatatgt ttggtctaga ttcttctact ctatttacta tatcagcctc tattccattt
    26221 tctcttaaac tattagctgt tccttctgta gcaacaaaag tatatcctaa ttctttcata
    26281 tcttttgcta tctctataaa ctcatcctta tcatgattat ttacagtagc aagtactact
    26341 cctctttcat cagacatatg tctacctgct gccaagaacc ctttatataa ggcttcttct
    26401 aaattttctc ctacacctag aacttctcct gtagacttca tttcaggtcc tagacttact
    26461 tcaactttag ataatttaga cattgagaat actggtactt ttactgatac taattttggc
    26521 tctttgtaaa ctcctgtacc atattctaaa tcctttaatt tagctcctaa catacactta
    26581 gtagccaagt caactattgg tactccactt actttactta tatacggaac tgttctacta
    26641 gctctagggt taacctcaat tatatataat tcattttgaa attctataaa ctgtatatta
    26701 accataccaa gcacattaag gtctaaagct atttttttag tgtattctac tatcttttct
    26761 tttatttcat cagatatatt ttgacttgga tacattgttg tactatctcc agagtgaact
    26821 ccagctcttt ctaaatgttc cattatacca ggtatcaata tatcttcctt atcacatata
    26881 gcatctactt ctatttctct accagttaga tatttatcta taagtactgg atttttatgg
    26941 tctctatcaa atgcatcttg taggtattga gataattttt cttcgttata agttatttcc
    27001 ataccttgac caccaagtac ataagatggt ctaactaata ctggataacc taactcatta
    27061 gcaacttcaa taccttcgtt aagtgaccat acaccctttc cttttggtct atttatatct
    27121 agtctctcta gtaaatcatc aaatttttct ctatcttctg ctgcatctat atcatcaaaa
    27181 tcagttccca atataggtat atttttttca tgtaaaaatt tagccaattt tatagctgtt
    27241 tgtccaccaa actgtagtat tacaccttct ggcttttctt tttctattat acttaaaact
    27301 tcttcttctg ttaatggttc aaaatacagc ttatctgatg tatcaaagtc tgtacttaca
    27361 gtttcagggt tattgtttat gattatagtt tctatgtcca tttttcttaa agattttaca
    27421 cagtgaactg aacaatagtc aaattctata ccttgaccta ttcttattgg accagaacct
    27481 aatactatca cctttttctt atcactaaca actacctcat catattgctc ataagttgaa
    27541 taatagtaag gagatatagc atctatctca cctgcacaag tatcaaccat tttatatgct
    27601 ggtttgatat tgtatagact tctcaattca tataattttt ctggacttat cttcattaag
    27661 tcagatatac ctttatcaga gaatcccttt ttctttaatt caagaagata gtctttagtt
    27721 aaatcttcaa ttttcataac ttttaatttt tcttcttgct ctactatcca tttaaattta
    27781 tttataaacc atttatctat acctgtaatt tgttctatca tttctacttt atagcctctt
    27841 cttatcattt cagctaagtc aaatagtctc tcatcatcag gtactacaac tcttttcttt
    27901 aattcctcaa tgcttctctc ctcagatggt gtgtgaacta atgaatattt tcctgtttca
    27961 agtgatctta tccctttaag aatagcagcc tcaaagttac ttcctatact cataatttca
    28021 ccagttgcca tcatcttagt tcctaatttt ctatttgctt ttttaaattt atcaaaaggc
    28081 cattttggta tttttgccac cacataatct atagttggtt caaaacaagc ataagttttt
    28141 ttagttactg cattttctat ctcatctaaa gtataaccta aagctatttt tgacgaaact
    28201 ttagctattg ggtatcctgt agcttttgat gctaaagctg atgacctact aactcttgga
    28261 tttatctcta taactgcata ttcaaaacta tgtggattaa gtgcaaattg aacgttacaa
    28321 ccaccttgaa cttctactgc atttaatata tctaaagatg cttttttaag cattgcacat
    28381 tctttgttac ttaaagtttg agttggagca acaactatac tgtctcctgt atgaactccg
    28441 actgggtcta tattttccat gttacataca actatacaat ttccctttga atctcttatt
    28501 acctcatatt ctatttcttt ccaacctttt atactctttt caattaatac ttgtccaaca
    28561 ggacttaatt gtagaccatg tgaaagtatt tctcttaatt cttcctcatt atcagctata
    28621 cccccaccag ttccacctag tgtgtatgct ggtcttacaa ctactggata accaattgta
    28681 agtgcataat caagacctgc ttgtaaatct gtaactatat cactaactat tacaggctga
    28741 tttatttctt tcattacttc tctaaatata tctctatctt cacctttttt gatagattct
    28801 actgaagtac ctattatctt tactccatat ttatctagta tgcctttttc ataaagttca
    28861 actgctaggt ttagccctgt ttgtccaccc atacctgcaa gtaaactgtc tggtctttct
    28921 ttttctatta ttttttctat aaattctatt gttaatggtt ctatatatat tttatctgcc
    28981 acttctttat cagtcattat agtggctgga ttactattta ttaatacaac ttcaattcct
    29041 tcctctttca aagactggca agcttgtgtt cctgaatagt caaactctgc agcttgacct
    29101 attattattg gtcctgaccc taatactaaa gttttcttta tactatctaa tttaggcata
    29161 aatttttctc cttatctaaa tggtttttac tatctgtaaa taatactaaa tctatattaa
    29221 tgctaagaat ttattaaata tacaatctaa atcttctttt gttgtgtatt ctggaatgta
    29281 ctgaacacta tatattggta atttcttatg tctcattcct tctatagtat tgtcatttaa
    29341 atttatgtga gttacttctg tattttctgg catttctgaa acattatatc catgattttg
    29401 agaagttata aatacctttc cagtttctaa acatttaact gggtgattac ctcctctatg
    29461 tccaaataca agctttgaag tctttcctcc taatgccaat gctaaaattt gatgtcctaa
    29521 acctattcct gtcaatcgct ttttgcctat taattttttt atattttcaa ctatatcatt
    29581 tatatcctct ggatttcctg ggccattaga taataagatt aagtcaggat ttatatttaa
    29641 aatttcttct actgtagtca ttgctggtac tatagttata tcacagttac aagctttaag
    29701 agactctagt acactatttt taacaccaaa gtctataact acaactttag ctccagtacc
    29761 ttttatattt tcaatttctt ttcttgtaac attttttact gaatctttgt tacaatgctc
    29821 ttctaattta gactgaacat cttgtaatga tacattttct aaagttataa ttcctctctt
    29881 agttccattg tttctaataa ttttagttaa tgctcttgta tctattcctt caagtcctat
    29941 taccttattt tgctttagat atgtgtctat atccatttca catctaaaat tatttgggtt
    30001 atcacttttt tctcttacaa taagtccttt tacttgcacc ttatttgatt caacatcttc
    30061 taaatttatt ccatagtttc ctataagtgg ataagtcata gtaactattt gtccataata
    30121 agttgcatct gtcaatagtt ctccataccc agccattgaa gtattaaata caacttctcc
    30181 aatagcttcc tttagatatc caaatgcttt tccttcaaat actgttccat cttctaaaat
    30241 taatttcccc ttcatataag ctacctccca aatattttat ctaagcattt tctatttctt
    30301 ttacaatatt aatagctgat tctcttggat tttctgcttt tgttataggt cttccaacaa
    30361 ctaaataatg agcacctttt tttattgcat ctgatggagt tacaactctt ttttggtctc
    30421 caacctctgc tgatttaggt cttattcctg ggcaaactgt tataaaatca gttccacatg
    30481 cttctactat tttatctact tcttgcgctg aacaaacaac cccatctatt cctgattctt
    30541 ttgccagttt agctctcttt atagctaact cttctgttgc catattacat cctatgtcct
    30601 gtaaatcttg gttgcttaat gaagttaaaa ctgttactcc aactactaat ggcttttcta
    30661 tatttaatct ttctgcttct tccctagcaa ttagtgcaca ttgtctcatt ccttccatat
    30721 ctgatacatg tattgtcatt aaccaaacat tatctctcac tgcagctttc actgcacttt
    30781 tcatagtatt tggtatatca tggaatttaa ggtctaaaaa tatttttttc cctttttctt
    30841 taagataatc tattgtttta ccttttgtgg caacgtattg ctctagccct accttaaata
    30901 tgtctacact atcttctaat ctgtctatta gttctttagc tttatcaaat tcgtcagtat
    30961 caattgcaac tatcaatttt tctttaccat ttatcatttt tacatctcct ttttaataaa
    31021 gcctaaataa tcataaaaaa atcctcacac caaaagaggc ataaggattt tatctataca
    31081 taactctatt cattagctat gagatatctt tcccagtctc tctggactag tttaaaagac
    31141 tttattatat ttaaattttt attttatact catcaaaaac ctctagaaga tttactagag
    31201 gttatatact aacattatct ataacccttt agtctctctg gactacttta aggaatttag
    31261 ctgtaatatt tttctttatt tatataactt tatgtatcta taggataatt taaagtttta
    31321 tcttttttat aattaaacca ctaattgctt tttatgtcaa taagtctttg aattattctt
    31381 catctttatc aaacaatttt ttctcaaaat atgattctgc attatatatt gcagcaactg
    31441 gattatgccc agttactcta tctttgacaa ctaaagttgt tataggagct tccatatatt
    31501 tcatggctaa agtatcgtga ccaacacata atccaagaat tacattaagg tcaaccttag
    31561 cttcatttaa aaataaagct tgtcctattg gattgcacat tacctcatct gaacactttg
    31621 atattgtttg agatgcttct agtccaattg tactcttagg cattgcccca tttttacata
    31681 taacagaaaa tacttcaaac ccatgatgtt caaatatttt ttgcactgtt tgcatttctc
    31741 tttttagtcc aatacaaaat gctatgccaa tctttttata accacacttt ttagcaaact
    31801 ctataatctc ttttagtcta gtatattgac aatacccctc tgcttcaact aatccagcat
    31861 tataagctat ttttctattc tcttcttctt cataaagttg ctttgctttt tgttgtagtt
    31921 caatttctct agttggacat atcttcatta atttatccat atttccatta gaacagcctc
    31981 taagattaca ttttccacac ttatacatat ccaatatccc cttccaaaat tttattcatc
    32041 ttgcgattct tctttttcta gagttacatt ttcttcttgg gtttcttctt ctggtttcac
    32101 atgctcgact acagttataa aatatgattt tcctatttta ttccttatag gaagtttttc
    32161 atatgaaaca tctattttat tttttataca agctatatca aaatgtctta aggctacacc
    32221 taagctaacc ttattcatat cttcacagtg tttcttaggt ctcttttcaa ataaatgaag
    32281 acctcgttca tccttatgga aataccacgg ttgagaattt tttacagatg gagctcttct
    32341 tgtcaattct aagatatctt tccactttct attcctattt atcttattta tcatatcttt
    32401 tactggcttt ctatcaggtc tagcatatct cgtcctaaac aactcttcat tttttgctgg
    32461 atatccaaat gctattatta tataaggatt ttctattttg ttttcatcat cttcttctgt
    32521 ttttattcca gctagataat ctaaatcatc ttcctcatct tcttcaaaat tcttattccc
    32581 aatatctaca aattccaaga tatcttctct ctttaagttt ccttccaacc agcaagtagc
    32641 tattcccaat gttgtaaggc gcaaaactac accttctgta gcaaatccta tattctgtaa
    32701 atagtcctca cctttgtctg atgtaactac tatataatga ggtgccttta ctttacattc
    32761 cttacccatc aaaaaatgta ttatatgacc tctatcaaca acatgtgctt taatatttaa
    32821 atcttcattt aagtattcta aatttttaca acttcttttt atttcttcca tcaatggctt
    32881 ttttatcttc ttactggaaa aatttcttac agattttcta taaaaaatgg cttcataaag
    32941 ctccatttat gccctcctaa ctgaaatctt ctgacgtata aactctataa atttatataa
    33001 atactttata ttttttatag ttttatttcc tttttcctcc tgccctattt tgaaattcat
    33061 aagaatatct actctagttt aatatagcat tcaaaaggct taaattctac cttatcaatg
    33121 tctatacatt agcataattt ttttatttga ttttctatgc taatttaaat aacaacacta
    33181 gctattacct ttttaattaa gctttgccag aaaaattttt ttcatttata aatatctatc
    33241 tcattattat attataagtt tttttcggta ttcttgtata gcttttcact aaaaaagtat
    33301 ttttatttct actttaatat tattatcaac aaaaaacata ctgcttaaat aagtttgtta
    33361 tcctcataat ctgttttaat taaaatattt tttttatttc tcaatctata aagtataaat
    33421 attttgaata tgacccaaaa tctaagtaaa gttataatat tttataagtt gttcaatatt
    33481 aaaaacataa gtaatgttat aatactttat aagttattca atattaaaaa tataggagga
    33541 taaatatgaa tttaaataga aatttagaaa aatacgctga attagcaata aaagttggag
    33601 ttaacataca gccaggtcaa attctactta taaaatcacc aatagaatgt gctgattttg
    33661 caagaaatgc acttaaagaa gcatataaat gtggtgctaa aaatgtttat atagagtggt
    33721 ctgatgaaga aagtactctt attaaatact tatatgctcc agatgaagca tttcatgagt
    33781 tccccaaatg gacagcagaa caatatgtag atatagcaaa agaaggtggg gcatttttat
    33841 cagtctatgc ccaaaatcca gatttattaa aagatgttga ccctgaaaga gttgcaaatt
    33901 ttcagaaggc atctggaaaa gctttaaaag aatggcgttc ttacactcta actgataaat
    33961 gtaaatggag tatagtctct gtgccaacaa aagattgggc aaaaaaagtg tttccaaatt
    34021 tatcagaaga taaagcaata gaaaaactgt gggatgctat atttaaatgt tcaagagtag
    34081 atggtcagga ccctataaaa gcatgggaag aacataatga aaatcttaaa tctaaaatgg
    34141 actttctaaa taaaaataac tttaaaactt taaaatataa atcctcaaaa actgatttga
    34201 ctctagactt gccaaagggt catgtttggc taagtggagc atccaaagac cctaatggaa
    34261 taagttttaa tccgaatatt ccaactgaag agatttttgg aatgccacat aagtttaaag
    34321 taaatggaac tgtttatagt acaaaacctc ttgtctatgg tggaaatata attgacaatt
    34381 tcttcttaac atttaaagat ggtaaaataa tagatttctc tgctgaaaag ggactagata
    34441 cactagaaaa acttattgaa acggatgaag gctctcatta tttaggagaa gttgctcttg
    34501 ttccatacaa ctcaccaata tccaatacgg atataatttt ttataatact ctatatgatg
    34561 aaaatgcttc ttgccacttt gcaattggtt ctgcttataa gacttgtctg gaaggtggaa
    34621 acaatttaaa agatgaagaa ttagatgaac atggtgtaaa tgacagttta acacatgtag
    34681 attttatgat tggttctgct gatatggata ttattggtga gacttatgat ggaaagcaaa
    34741 tacagatttt taaaaatggt aattgggctt tttaacatat gattttaact aaaaagggct
    34801 gatggaagtt ataccagact tcccagccct ttttaatgtt gatgataaat attttttata
    34861 ttaattaatc tcattattat agattaattc ctattgtttt aaacataagt atctaaaact
    34921 tatatttata ttatatataa aatatcctat ttagtaaaac ctttatttta atttatatgc
    34981 taaaaattta actttgctat ttcaacagca gactttgcat cctttgaata ataatcagca
    35041 cccatttctt cagcgtattc ttctgtcaaa actgcacctc ctacaaatac ctttgcattt
    35101 atctcattat ctctcagtgc ctgaattgta tctttcatac tttgaacagt cgtagtcata
    35161 agtgcactta atcctactaa ttttatattt ctctttttag caacttccac tacttcttca
    35221 atagggacat ccttacctaa atctataacc tcatatccat aattttctag cattattttt
    35281 actatatttt tcccaatatc atggatatca ccttttactg tagcaactat tatttttcct
    35341 ttagatacat tattgctact tttacttaat aaagtttcct ttattgtatt taatgaaact
    35401 tttaccgttt ctgctgattg aatcaattgt ggtaaaaata tatctccact atcatatctt
    35461 tttcctactt tatcaagagc aggaattaaa atctcatcta atatataatt ttcatccttc
    35521 tcttttaaaa gattcagtgt tacatttttt gcttcatctt ttaatcctct ttcaactaaa
    35581 atatccaatg gcaaatcttt gtcaatcttt gtagatgaat ctgatttttt tgaattattt
    35641 atattactat attgatttat atagtttata catcctttat ctgtgttatt taatatttta
    35701 aaagaattga tagcttccat catactatat tcatttggat ttataattgc taaatcaagc
    35761 cctgccccta aagctaatgt tagaaaagaa gcattaagcg ctttcctatt tggaaggcca
    35821 aatgaaatat ttgaaacacc taatatagtt tttactccta gtgtctttac cattgttata
    35881 gcttttatag tttctattgc ttcttcttgt tgtgctgaaa cagttaatga taaacagtct
    35941 ataaatatat cttttggttt tatcccataa gaaactgctc tattgactat cctttttgct
    36001 atttcaaatc tttcttcagc ctttttaggt atcccctttt catcaagtgt aagtccaacc
    36061 acacatgaac catacttttt aactattggt aatatacttt ctaaagactc ctctttgcca
    36121 tttacagagt ttactattgt tctaccatta taatatctaa gtccttgctc taaggcctct
    36181 acattggaag aatcaacttg taaaggtgta tctactactg cttgaatctc tcttattaat
    36241 tttggcatta gttttttttc atcaatctct ggaagaccaa catttactcc aagtatttgt
    36301 gctccagcat ttacttgttc taaacctaaa tttatagcat aatcaacatt ttcatttttt
    36361 aatgcttctt gtagagattt tctacctgtg ggattcaatc tttctccaac aactgtaggc
    36421 gattgtactt ccacaaattt tgatggtgaa cagactacac atttatcaat tttctcgata
    36481 catccctttg taacagaatt tatattctca gatatttctt ttataaaact tggattcgta
    36541 ccacaacatc cacctattat agtagcacca acttcaacga actttttaac cccttcaaaa
    36601 aattcttttg catcaacatc atatatagcc tctccatcaa ctatatttgg aagacctgca
    36661 tttgcttgaa ccataacagg gactgtagct cttgatgcta ttttttcaac tattggtaaa
    36721 agttgtttag gtcctaatga acaattaact cctattgcat ctacacctag accttctata
    36781 gtagccacca tacattctgg catacatcca gtaaaacttc ttccaccttc atcaaatgtc
    36841 atagtacaga aaataggtaa atcagagttt tcttttgctg ctaatacagc tactttagct
    36901 tcatataaat ccatcatagt ctctattact attacatcaa ctccagattt aacgccttgt
    36961 agtacttgtc ttttaaatat ttcatatgct ttatcaaaac ttaaagtacc cataggctct
    37021 agcatttctc ctataggacc aatatctaaa gctacatatc ttggtttagt attatctaca
    37081 ttttctatag cttttctagc caccaaaaca gcattgtcaa tcacttcttc tacagtatac
    37141 ttactatcta attttagttc attacatcca aaagtatttg taagaattac attcgaacct
    37201 gcctctaagt atgctgtatg tatttctata agtttatctg gattttgaag accaaatact
    37261 tctggatttt cacctaactt taaccctttt tgttgaagca ttgttcccat tgcaccatca
    37321 aatattaata tattattttt cagatattgt cttatttcca caagagttcg ctccttttct
    37381 atatgtacag tttctatagt ttttacaatt ttcacacgac ttttctttct tagatttatt
    37441 tattcttgta ttagaaatac ctataatagc cactactgac tttctgggta tcataattcc
    37501 attagaagta attgttagac ctatttgttt ttgagcattc aaaacgttta atatatctat
    37561 atttttttct attggtaaat ctccatatcc tgggctatat cgcatagtta taaatttatc
    37621 ttgctttaaa aaaacctttt ctaaattatt ttgaacaatg tcacacactt cttctattgc
    37681 tgttgttcca catgaatcaa ttattagtcc ttttgttaaa tttgaataag agtgtcgtct
    37741 tatttctttt tctatatcta tccctatagt agctgccata attacacact cattacattc
    37801 ttttaaaagt tcatatacat ctttactctc taatgttaaa tttgttccct ctaattctac
    37861 aacattatct ttatttattc taataggata aatcctagat aaatatctag gatttatttt
    37921 atttttagtt atagctacac attcatcaat agtatattta aggtctttat ctatctcttg
    37981 acctctgtat tgtaaatatc ttaaaacttc actttgattt atgcttatat cttctcttcc
    38041 aattaaatta ttctccatat atatttaaag aaaatccttt atctaaaaca tcttttgcat
    38101 attcctttgc cccaaataaa gataagtctc tgtagtttcc acattctata gctgtagctg
    38161 ctggcatttt atctgattca agaacttttt ttaatgcttc ttctaaacca atcttaactg
    38221 ttttggcatc tacatctccc catagtatta aataaaatcc tgttctacat cccattggag
    38281 ataagtctat tacaccatct aatgtatctc ttaaataagt agctaaaaga tgctctaaac
    38341 cgtgcatagc agcagttcca aaactttcta catttggctg taaaaatctc aagtcaaatt
    38401 tagttacttt atctcccttt actccatcaa gtacactaca ctttcttaca aaaggagctt
    38461 ttacttttgt atggtctagc ttaaaacttt ctactttttc cattatttaa tcctccatta
    38521 tactttaaaa tttgttacat taattctaaa aaaaactctt cctatctatt attgataaga
    38581 agagtatatt atttcaagtc atctcatcgt tgttagcagg accacctgat aaaaggttgg
    38641 tataggatca ctgagctaac tctctcccct aactcttgat aagtatctta ttaaactgac
    38701 ttaaattata tattactttt ttatttttgt aaacacttta ttgttataat atttatatca
    38761 cataatcgta cttttatttt ataactaagc tgattgtatt attggcagtt gtacttcaaa
    38821 cacagtttga tatttatcgc ttgaaacagt aatttctcca tcatgtaaat ttacaatatt
    38881 ttttgtaatc gcaagaccca atccagaacc tccaacattt ctatttctcg atttttcaac
    38941 tctataaaat ctatcaaata tatttggtaa atctaacact ggaattggtt ccccataatt
    39001 tatcactttt acaaccgcca tatcttcctt tttctctgtg actatatcta cataatgccc
    39061 atcttttcca tatttcacag cattagttat aagattttca aatgctctta ccaatttatt
    39121 tggatcagct tttacaatta atttttcttc attaaaatta attctactaa ccataaattc
    39181 ttgtttaaaa taaatttcaa attgagatac tacttgactt aataactcta ctaaatttat
    39241 ttctattttg tttaatttta ttgtattatt ttgcatttta gtcaattcaa acaaatcatt
    39301 tataagaaca tttaaatttt tcgatttttc atatgcaata tcaacataat acatcatctg
    39361 aacttcatct ttatacttac cttcttctat cactcctaaa taacccatta tggatgtcaa
    39421 aggtgtcctt aagtcatgtg acacatttgt tattaaatct gtttttgtct gctgagcttg
    39481 tctctcttca atagttatat ttctaagttg atggactata ttattgattt tatgtgctag
    39541 ttcatcaata tcatcctttt ctttatcctt taatatatct aaatcatact caatatctat
    39601 acttttttct aagttacctt catccattaa atttacagca cttttaatga tttctaattt
    39661 ttgatttttt ctattagtct ttcttataaa atctacaatt tgcttatttc tcttgtacga
    39721 tataatggaa taaattgcta atgatgtgat tgtaaataaa aatggataaa ataaatcata
    39781 ccctataaga cttctgaaat ctcttgcttg atatacaagt tgattaacta cagacatatc
    39841 actattgaca tataatgatt ttattatata catacttgtt aaaataagca ttgaagttaa
    39901 agcacagctt attataagat ctaatatggg tcgaaacata tgatttaata aacttttatt
    39961 tttcaatttt atatcctact ccccatacag tatgaattat tttttctcca tctgtatatt
    40021 tttcaatttt atcccttatt ctgctcatat gaaccattac agtattatta gattggtaat
    40081 atttctcttt ccataccttt tcaaatattt cttctgtact atatactctg cctttattat
    40141 ttgctagtaa atataatata tcaaattctc ttgatgtcaa aattatttca tttccagcca
    40201 ctgttacact atgtttttcc ttatctataa ttactactcc aacctttatg acatctttat
    40261 cttcattatt agcaccacta aaataatatg ctcttctaat cagcgacttc actctcacag
    40321 taagttcaag tgggttaaat ggttttgtca aataatcatc tgcaccagtc attatccctt
    40381 gtattttatc catgtcttct gattttgcac ttaacataag tattggtata ttcaaacttt
    40441 ctctaacttt tctacaaact tctatcccat ccattttagg catcattata tccaatatca
    40501 ttaaatgaat attttccttt tttaaaatat ctagagcctc ttttccatct tcagctttaa
    40561 ttacattata tccactattt aaaagatata tctctataag gtctcttatt tccttttcat
    40621 catccacaac aagaactgta ttattcattt ttacacatcc taacttaatc tttgtatata
    40681 gctttattca tataaaatta tactatattt tagttagtgt aatctataaa tttattaatt
    40741 tctaaatttc caatataata caaattattt acacatttat attatttatt ttgtaaatat
    40801 tcttctaaag ttaatccttt tgaataaatt tcctcggcag catccttacc aacatatcta
    40861 atatgccatg gttcatatgc tgtacctgtt atatcttctt taccttcagg atatcttatt
    40921 ataaatccaa acttgtgagc attttctgcc aaccatttag cttcttttgt acttccaaca
    40981 aaccatcttt ctggatttgt taaatcaata caaagtccag tttggtgctc actgtaacca
    41041 ggctttgcta catatgcatc tgccttttct cgtccctgag attttaccct tcttgtataa
    41101 gtatccagtt gagtatcata agatctgtat gctgaagacc ctaaaaactg tattccttct
    41161 gaattagctt gcttaactaa atcttcaatt gcctttgcag gttctcctgc catttgtttt
    41221 tcttcctctg ttgcttcttc aacaaaaggt atgtttacct tagtaatatt ttcaggagta
    41281 tagtttttgc taattccatt tgtcttgttt accaataata tatttgatat actatttgag
    41341 tgatttttat tttgagaatt tgcctcaaca tcttgttctg aatcatgttg agcagctact
    41401 attggatctt tatctttctt aagtaatcta tctttagata taaatacact tataaatact
    41461 actaaaataa tggctcctat taatttaaat tttttcataa ttaaatcttc tcctttttgt
    41521 tatgtttgct ataatgttaa ttttagagta taagacttaa gtattttata agtaaatctt
    41581 aaataaatct taaatttaat atttttaaag gatggtgata aaatgtctat acaaaaactt
    41641 ctaagtaaag ctcgtaaggc tatacaagat tttgatatga tacaagaaaa tgataaaatt
    41701 gcggttggac tttctggtgg aaaagatagt ttaactctac ttcatatact taaaagttat
    41761 caaagatttt ctccacaaaa ctttgaattg atagccataa cattaaatac tggaggtgtt
    41821 gataattctc cactagataa attgtgtaaa gaaataaatg ttccttttta tgaatttcaa
    41881 actgacataa aagaaattgt atttgatata agacaagaaa agaatccttg tgcattatgt
    41941 gctaatttaa gaagaggtgc tttgaacgat aatgctaaaa aacttggatg taataaagta
    42001 gctcttggtc atcataaaga tgatgcaatt gagacatttt taatgtctat gttttatgag
    42061 ggtagagtaa attgtttttc cccaaagaca tacctagaca gacaggattt aacagttata
    42121 agacctatga tttacataga agagtatatg actaagaaaa tttcaaaaga ttctaactat
    42181 cctattatca caaatccctg ccctgcaaat ggtcatacta ggagagagta cataaaaaat
    42241 cttattgcta atttaaataa agagatgcct gattttaaaa gaaatgtatt tggtgcttta
    42301 aataattctg agaaattgtt tatatgggat aaagagaaga taaaaaattt taaataataa
    42361 ttaaaatttt taaataaaaa agcaagattg tattaattgt aaataatctt tatataatct
    42421 acacaatctt acttttttat ttattatatg ttattatctt taatctatat tttaatagtt
    42481 cttaatagta tttattttgt ctaatacatt aattataact taaatatttt atatattaat
    42541 ttaatctatt aattcattca atctactggt ttaatccatt tttacagtat atacatctac
    42601 atattataaa aagcaaaatt atattttcta aatattttat gttagattat aatattaaat
    42661 tctattatct ctggtatatt aaaaaatcta aatggtaact ttgtacttcc aagacccgaa
    42721 ctcacatata aatgatttcc atttatatca taaaaccctt tagtatattt ccttccatat
    42781 ggaggagtaa ctaatgctcc cttaataggt aatcttattt gaccaccatg actatgccca
    42841 gatagaacta aattcattgt atctttacta agcatatcta ctaaatctgg ttcatgtgct
    42901 aacaataaat taaaactttt attatctatt tgactctcca gatattttat attaggattt
    42961 ccatttaata tttcatcaac acctaatata ttaatatatt tatcatcttt taattttatt
    43021 tttttatttt catttactaa aagattgaag ttggaatttt tcataatctg cctataatac
    43081 ctaggaagtt tatacatata atcatgattg ccaaatactg aaaattttcc caacctagca
    43141 ttcatacttg ataaaatagt tgtaatttca tctacacttg gatttttatt ggagtaatca
    43201 attaaatcac cagtaaatac tattatatct ggattcaaag tgttaatctt attagctact
    43261 tttttgagat tcttagttga ataaaaacta cctatttgag tatctgaaat ttgaactact
    43321 tttacacttt cagatttact ctcaattgta tcatttttta tcaatgctcc attttcactt
    43381 atattatatt ttttcacaat taatagactc ggctctatcg caactgagta tataaaaatc
    43441 aaaataaata ttaagataaa aaattttaaa aaatacttaa tttttgagtt caaaatatct
    43501 acctacttat ttattttttt aaactatttg cattacagac tcaactttgt tcatttttct
    43561 gtcatgcatt tttggcttat ttgctgaaaa ccatttatat gttgctgtaa gaccttcttt
    43621 tagtaacacg tttggtatat aaagcccatg ttcaataagc ttatttatat caagattaaa
    43681 gtctatattt ctaaatggga aatacgttct ttctctaaac tctacttttt ccatatctac
    43741 atattttatt ataggttctt tgccaataat ttctccacat gtatatataa ggtcatccca
    43801 agaaattaac tgtggatttg tcacattata ggcctctctt acatgaggat ttttcattat
    43861 gctttccaat acctttacca aatccccaat atatataaat tgattactaa cttgttttcc
    43921 ttttggtact ggtatctttt catttttttc tattttttca aagaaataat actctctata
    43981 taaattatta ttttcaccat atatgtaagt tggtctaata attatatatg gtatagggct
    44041 atttataata aaatcttcag cctctttttt atttaaccca tattttcccc agttttcatt
    44101 ttcacctttc tcattttctt cttttatatt tcttccacta tctttatata ctgctcctgc
    44161 tgacaatact atatactttt ttaagttatc catgcttata aaatcaatca gattacttac
    44221 atcttctttt gtatatgctg tcatatcata tatgtaatca tattttcttc ctgtgataat
    44281 gttttccata tcttttcgaa cttttctatc acaaattaga tgttctttaa aaccattata
    44341 atctatttcc tttttaccat ttgtaagtat atcaatttga tacccacatt taatcaagcg
    44401 cttagcaagt gcagaaccta tgaagtcgct tccacccata attaaaatag atttcatttt
    44461 aagtgcatcc tcctcttttt taaatgactt ccttaactat aatatcatat ttttaaatat
    44521 gcgacaccat tttgtaatat ttttgtctat aaaagatatt tataacaaaa tatcttttat
    44581 agactctaca ttttcgacaa tataagtagg ttcactttca ctaatctctt caaaagaacc
    44641 atacccatac aatactccaa tagaatctat acctattttt ttagctccaa ttatgtcata
    44701 ctttctatct ccaaccataa taactttatc tttatctttt acattacata ggtctagtac
    44761 atattgtata acttcatttt tattcactct tgtaccatca agattgctac ctgctatata
    44821 tttaaaatat ctatctatat caaaatatct aagtatagtt tctgcaaaaa ctgttggctt
    44881 agaagttgct actaataata ttttaccatt tttatagagc atttctaata tctctttcat
    44941 attctcataa attttatttt caaatatccc tttatcagca aaatattctc tatacttttc
    45001 aacagcttct ttagcttttt tatcttcaaa tttataatac tccttgaaag tatcatgtaa
    45061 tggtggtcct ataaattggt ctaagttttc caaatcttct tttatgccaa agctatttaa
    45121 agaatactga atagactttg ttattccttc tttagggtct gtaagtgttc catctaaatc
    45181 gaataatact atttcataat tttttttcat atttcagtcc tccatatttg taaaaagtaa
    45241 tatcttatat attaagttta tctattttaa aaagctatag taatagaaat ttctaatact
    45301 atagcttaag taccaattaa atcttatata tttattttaa aaataaattt aaacagctct
    45361 atttaactgt tatagctgaa actggacatc cttcttcggc ttctttacat aaatcttgta
    45421 aatctgctgg tatttctcct tcaattgcta ctgaatgccc ttcatcgctc atactaaaaa
    45481 cgtctggaca tgttcctata catgctccac aagctacaca tacatcctta tcaacaaatg
    45541 ctttcatact aaaatacctc ccctaaaatt attatttttt aattgttgtt tccttagata
    45601 tttatccaat atataccaat ttaaacatat atttattatt ttatcactat tgtttagttt
    45661 attatagtat tttcgtatcc atgtctataa acttcactgc ttttcatcca tgattttaca
    45721 gtatattgca atcttaattt tttatttaaa acaagttcat tattattgta gtcttttata
    45781 atatcattac actcctccat tgtgaatatt gtaaaatcac cttctcctcc aatctttatt
    45841 ctgcctttat ttccatgtaa atcgaaaatc tttgttgggt attcagttac tttttctata
    45901 cacttttcta gggatatccc aagttccata attttattta taacatttaa aagagaatag
    45961 tttacgtctt tcatgttttt tatatgcata tcagtactta ttaggtcagg ctctaaacct
    46021 tgctttattg cttttttaaa aactttaaaa tcaaattggt ccactccatg acatacatca
    46081 aacaatactc ctctattctt tgcatttatt actgattttc ttaatgtgcc agattcttta
    46141 aggattccat ttgtactatt tccatatacc ggtgtaacca catctccttt tcccaaaata
    46201 ttcattactt catctatata tgatggatac tctcctatat ataccactaa gggtaaattt
    46261 aattttttag caacttgctt tgatttctta attatatcta taccactttc attagattga
    46321 aatttactcg cttttacttt tatacccttt atcttttcta tatttttttc tacaattgac
    46381 tcaaccttgt caaaatttat aacatctaaa gaagttgatt cattcaatat atttagtccc
    46441 tcatttgtaa catttaaaaa agaatatact tctgttttac actcatccat atactttttt
    46501 ttaaaatcat agtagtttga aggaccagct gtacctgcat caaatataac tgtagaacct
    46561 ttttcaattc caattgaatc agaatccata cctgtaggta attttctctt ataacaatgg
    46621 gtgtgaacat ctataaatcc tggggaaata tacatattat cggcatttat tatctcgaac
    46681 tctttatttg taagtaattt ttcttttctt attttctctt taaggttcaa tccaatagca
    46741 tatattttcc catgtaaaac caagatatct cttttttcaa atttgatacc atctgttgga
    46801 gatattattg tcccatttct tataagtaaa tttttcattt attccacatc ctaattacaa
    46861 tataatttct ctaatagctt ttcttacata aagaaaataa gcctaaaaat aaaaatattt
    46921 tagacttatt cactatatta tactatatta attatattaa attaaataaa atctttaatt
    46981 tactttaaga agcaattaag tatttgttgc tcggcttctt cttcagtaag acctaatgtc
    47041 atcatcttta gtatttggtc tcctgcaatc tttccaatag ctgcttcatg tactaaaaga
    47101 gcatctggat gatttgcagc aatttcaggt atagcactaa tttgagaatt tcccataatt
    47161 attgaatcac attgaacatg tgcagaacaa agggaatccc caataacaca aggattaaac
    47221 ttttgcttag aattatcttg cccaactgta cgagaaacta cttgagcaga agagttttca
    47281 ccctctaaga taaccttcat attagattct gcaacttgat taccatgagt taataatttc
    47341 tcaaatacaa ctattttagc tcctttacct aggtttgcat ccatttctct tttagttgaa
    47401 tctacaccct taatttgaat tgtttccatt tcacaatatg aatcctcatc catattgaca
    47461 atagtaactg gatttaaaat tctctttcct tctccatcac cttcaccata atgtttttct
    47521 acatatttta ggcgagctcc tttacgtaag aaaaatctat gtataccatt atgttcacta
    47581 tcatcacaac cacagttatg tataccacac ccagcaatta taacaacatc tgcattttct
    47641 ccaatttcaa aatcattata taccatatcc tgtacaccag cctctgttac tattactggt
    47701 atatgtacat tttctccttt tgtatttggc tttactataa tatctattcc aggtttatca
    47761 gttttaggaa ttatagttac attttcagtt gaatgtctct ctactccttg cccattttta
    47821 cgtatattgt atgctcctaa tggagttcca cgaagttcag caactttttc taaaagattt
    47881 aaatctattt tgttcattta atttgccttc ctttctaatt aattcatgtc tttcattaat
    47941 tcacaacttg aattaacttg acacatgatt tcaggtaata tagcctcttt tgttccatgg
    48001 ctttttactg aaccatcttg tagaacaatt atttcatcag ctaactctaa tattctttct
    48061 tggtgagaaa ttatgattat agtctgatta gactcttcat gtattttctt aaaactttca
    48121 ttgagttttc caaagctcca aaggtctatc cctgcttcag gttcatcaaa tatagaaacc
    48181 tttaaatctc tagcaaaaag agttgctatc tcaattcttt tcatttcccc tccagaaaga
    48241 gagttatcaa cttcacgatt taaatagtct tttgaacaca atccaacatc tgtaaggtat
    48301 tggcaacata catcagtaga caatggtttt ccatgagcaa gtgtaagtaa attttcaact
    48361 gtcatacctt ttattcttgg tggttgttga aatgcgtatc ctattccttt ttttgctctt
    48421 tctgtaatac taagatttgt tatatcttct ccatctaaaa ttatctgacc actcgtagct
    48481 ttttcaattc ccataataag cttagcaatt gtagattttc ctccaccatt tggacctgtg
    48541 ataacaataa gttttcccct ctcaaatgaa aggctaacat catttattat tcctagttcc
    48601 tcattgttag actcaacatt aaaggaaaga ttttttattt caagcataat acccctcttt
    48661 tctataaaat attacattaa tatccattct tttattatat cagtaatttt tatatatcag
    48721 caactctaag gagataaaat gtatattaaa taataaaaca aagttattga aaagtaaaaa
    48781 agatgagttt tatgtaaact catctcagaa atttagtata tcaacttata tgtatttaaa
    48841 atttaacctt ctatatactc taatattatt ttttgattat cttttaacat aatttcttct
    48901 tttttagatt ctattccatt cttttttatc tttacctcag ctacaaaggc atcattacct
    48961 tcaaccattt ctaatagttc atacgctttt ttacgaccta ttttagttgc tatatctttt
    49021 agtactgcca ttttcttaaa cattttatca cctctactca aaatttacac ttaattttta
    49081 attatataat tctaatccta gactagttta taataattat tgtctctaat tatatcagat
    49141 attgagtaaa tttaatactt tgtaaaatta tttatcctct ctgaccaaat aatcatttcc
    49201 atcttggtct ttaaaagaaa acatccttcc atatggcatc tccattaatt ccccaacctc
    49261 aacctcgtta tttgccattt gttcataagt tccattaata tctcttgtac ttaaaattat
    49321 tgatggatga gatacattta tctctttgtt ttgcttcatc attagatttt tatcatacaa
    49381 cacaaatgtt gtaaattcat ttttacttgg tgctacttcc acccatttca tatttggacc
    49441 catttccttt tcataagtta ctacaaagtt caatttttct gtccaaaata actttgcatc
    49501 ctcttgatta tttacataga gagttatttt tcctatttta tttatcataa caatacccac
    49561 ctttattatt aaataaatac ctaatatttt ttatatttaa taatataccc atatatatta
    49621 ttttctatag atatatattt ctaatattta tcgccaaatt atgtaattct gctcaatata
    49681 taatttataa tatccatact attatagcct ttatttatgt aatttaatca ccttacatat
    49741 catacttata attatgataa taactacata aaaaatcact tctaaataga gttatccatc
    49801 taaaagtgat ttcatatatt ctatgcaact ttgtgtttta gccactttgt tcctcttaat
    49861 cttaaacaat ataaagtccc tctaacaagc caatcagtat acatacctat ccaaattcct
    49921 aacactccaa attctaatac aacccccaat acgtaacctg taaaaattct aaaaatccac
    49981 atacctatta ctgcagtcat cattgtatat ctagtatcac cagctccctt tagccctgct
    50041 gataatacaa aggatatagg ccatattacc atagctaaac aatcacttcg aattaattgt
    50101 gttgagatgt caattacact agcattttga gtatacatac ttgaaatcca gggtgcaatt
    50161 ggtataaata tagctcctac tataaataaa caaattgttg caaatttagt taaatataat
    50221 aaagtactct tagctccttt aatatctcca cgtcctatat actgtcccac caaggttgta
    50281 gcagacaaag ctaaagcatt cccaattaca tttattatgg atgcaattga cattcctatt
    50341 gcattggatg caattgctga tgttcccata gtaactataa atacttgaac aatcaattta
    50401 cctgcattaa aaagtagttg ttcaactcca gctggtattc ctatattaaa tatagacttt
    50461 tgagtttcaa catcaatctt aaatggaaat attttaccca tagatataaa ttcgtttcct
    50521 ttaaataaag ctcttataat tactatacat ccaattattc tagctatagc aatagatata
    50581 gctgcacctg ttatgccata ggatggtgtt tgcaaattta ttcctagagg atttaaatca
    50641 ataccatata taagtatata tcccaatgtt acatttacta tattcataaa catagtcaca
    50701 tacataggtt tctttacatc tccacaccct cttaaaatac catttgctat ttgttgtgtc
    50761 gcaataaatg gatacgtaaa taatgtaaac tctatgtata attttgcatt tgattttacc
    50821 aattcttctg cggaaccata aaataaatta attacaccag ttctaaaaac ccacatcaat
    50881 attgttattg taaaggataa tattactcca gacactagag cctgcttaat agtttcattc
    50941 accttttcac tatctttttt tcctatttgt tgagctacaa caacagtagc tccaacagat
    51001 agtgcagcaa atacagatat aaatatattg tttattgaat caaccattcc aactgcagat
    51061 acagcttctt ccccaatatg acctgccatc attgtattaa atactcctaa aagcatgaca
    51121 aacgtctgct ctatcattat aggaattgct aactttataa catttttcct tataaacatc
    51181 cctattcctc ttttccctct ttatataaat ttaattttta attagtttat cgaactgtaa
    51241 attgacaatg caaagtttag aattgtattg ttcgcattat aatatactgc tttcaaataa
    51301 tataaatctt gtttaatatc atttgaacac agtatatctt tataattatc ttatgtttat
    51361 tattttttat ggataccaca tttaaaatat ttattaattt aatataaata ttttccgtta
    51421 ttattaaaag tagctatatt tcgttaatat tgtatcacta tcgtgtagca ataacaataa
    51481 gtacatttta tatatttttt ataatattat ttaatcatcc aaacttttac cactttttca
    51541 atatataaaa tattacactt tagatgtaac aaaatcgcat ccatttaaat attgtatttt
    51601 atagactaat tttaggatgt gatactttgg ttaatttaaa tgcaaataat ctaaatgtaa
    51661 atatggttta tcctatttct tccacagcac ccatacgttt cagaagatat actgtttcat
    51721 atttagaaaa catggaaaat atttatttaa ctatagcaac atactttgat acacttggtt
    51781 taggcacaat gaatatcgac aaagtttatg gtcaatgggc ttggtttact gataatatat
    51841 atgatttaaa tctatttgtt tttgtaggaa attatcctta tcaaattgct aaaaacagat
    51901 atgatacatt tgtagatatt ttgccagtag ctattacttc tattgtcaat ggagatagag
    51961 tatttttaat ttcaaatcct tgtttgttaa attcaaaaat tacagttaga tttatatcta
    52021 gtcatcctac tcttaataga acagttgatt atggcaatat tagagatttt attttttaaa
    52081 actgcaacat gatgtgctat taaaaaatac cctaaattta atgatattat taaataacta
    52141 gaatattttt aattggcaca tcatgcctag ctattataaa ttcataatat tattttactt
    52201 aagataagta ctgtatttta tctacttgta tatttttatt tacctagttt ctcagttcta
    52261 tatcctattt ctttattagc tagcttataa ccttcttgta tcatttcttc tctcgttaat
    52321 gcactcccta ataaattgtc aatactcagt tgctttctat cactaaaata cttatccaat
    52381 aatgattcct ctatatcttc ctgatatgcc aaatttcctt catgcattat atattttaca
    52441 tcaaaacctt ctttatttaa tacttttccc accaagatac ttctatgaca tcttataggt
    52501 tcttgcatag ctcctaataa aactatccta taacccattt gacaaccctt ttttaatctt
    52561 tctattccgt ttaaaaaaat atcttctttt gctactttct caaaatctgc ataaccttct
    52621 tgcgtatata catctttatt agttctttta gctccaaatt cttttcccat gtatatatat
    52681 ataaatccgg aatttctaag agattcgtta aatgcctctt tattgtattg aatattatat
    52741 ttagaatatg gagtttctct aatgtcaact acacaattta tgtcatattt ttttatcata
    52801 ttaataagct tatcatatgg ataatttgaa tgtcctattg cgaatatttc catataaaca
    52861 ccctcctatt ctttgaaatt agtatctata tatgtagata cccgatttac ttaaaattaa
    52921 ataaagtatt actttaaata cccaatatct ttttttcttg atattgaata ttttttactt
    52981 tatatcctta aaaaaatata caactttata tataaaacat caatatttat atatattttg
    53041 tatattaaat acaattattt ataaagtctt taataaaaat ttataaatag agcatcttta
    53101 gaaaactatt ataataaaaa aataagaggc tgtctaaaaa tatagataag ccttttttca
    53161 taatcgtaat tataaaaata tacttgattt catatgaaaa agagtgtctc ataaactaaa
    53221 atgtttactt tgagacacca ccttttttat tcttatttat actttgtaga catattataa
    53281 tatgcgtttc actggtatct gaacttctgt caaccagtct tcttcactct ctttattcca
    53341 tataccatct atataagatt ctctaggatt tccaaccact ttatatttat tgtcctctat
    53401 ccatttaaat gcatctgcgt aagtttttcc taatgttgaa tatggaccat tgtgtaatat
    53461 acaaactgca ttttcaactg catctaattc tttaaatttt aatatatccg tatcttcctt
    53521 taaatcattt atagcttgac acatttcaac atccatatca gtctctctat attcaccatc
    53581 atgatatata ttaaaacaat aatcaggaac tgcacatgta caccctgttt tcatcatttc
    53641 ttcagccatt acatttggaa atatattaaa aagttcatca taccccttta acacttttct
    53701 catagaagca actttaactt ttggtaaaga cttaataatt acatcattca tattgacttc
    53761 ccctttcaaa ttcataaggt aattacgtag tttaattatt ttatattctt cacttttaat
    53821 tacatccaac atttcttttt ctttattcat taaaatattt tcaacttctt caggattttc
    53881 tacagctttt tttatctcag ctaatgataa acccatttgt tttaaagtta ttatctgatg
    53941 taatttaggt aattgctctg ttgtataata cctatatttt gtaaattcat ctacatattc
    54001 tggttttaac aaccctatat cttcataata ccttaatgtc tttgtagtta ttttatttat
    54061 tttagaaaac attcctatag aaaacatctc atcacctgcg cattagattt gattaattta
    54121 agtttaaact ttccccttag gttaaagtca atacaaaata aattataatt gtattaattc
    54181 ttcaattcta tctccactca ataccaaaat atcccctgtt tttataatgg ttgaaccttt
    54241 aggtattaaa acctgttcct ttctttttat cataactatt aatatatcgt ctggtatatt
    54301 agaatctatt atactcttat tagcccattt attaccctca tcaacattta cttcgataag
    54361 tttagagcta atttctccat cataatcatt aaatgttttt aatacaggtg tattatcatc
    54421 aactaaatcc aactttctgg ctacagttgg tatcaatgaa ccttgtattc ctactgaaag
    54481 caacgctata aaaaatatta tatggaatat gtcattctct atggctactc cttgagtaac
    54541 agcataaatt gcaaaaacta ctgaagccgc acctctaaga cctacccacg atatgaataa
    54601 tttttcttta tttgagaatt taaattttgc tagaactata aatgttgcta atggtcttgc
    54661 aataactatc atgaatatag atatggccat tgctatacca aatacatttg gtaacttaga
    54721 agggaaagat aacaatccta acataaagaa taacattatt tgcataatcc atgaaattcc
    54781 atcaaagaaa tgaaccaaac tttttttaag aggtatccta ctatttccta taataatacc
    54841 tgaaatgtaa acacttaaat atccatttcc tccaagatac tcgctaaatg cataagaaag
    54901 tattgcaata gctgttataa atatagtata aaatccttct atctcgaagt ttgaatgtct
    54961 taaaatataa acagataatt ttgctagtat aaatcctata gctaacccaa aagctatttg
    55021 ttttattacc attggtgcaa taagttctac cccagaatta ttcatcaatg tcaaaagaat
    55081 tagtgtcaac atatacgcta ttgggtcatt acttccactt tcaacttcta ataatgatgc
    55141 aagtgaaccc tttaagttta gtttttgaga acgcaacaca gcaaacacag atgcagcatc
    55201 tgttgaagct gcaattgccc caattaataa accttcaagt aaactagttc ctattaccat
    55261 gtggcaaaac atacctgtaa ccccagccgt aattattaca cccagtgaag acattaatat
    55321 agaaggtata gcaacaggtt tagccatatt ccagtttgtt ccaaaccctc caaaaaacat
    55381 tataaatatt agtccaatag aacataattg ttttgttagt tcatagttat caaaatagat
    55441 tcctaccatc ccatcagacc caaataacat ccctagtaag ataaaaatca ataatattgg
    55501 tactccaaat ttatacaata ccttgctaga agttatacaa atcaatagta ctaacccact
    55561 tataatcatt aattctgtca tagcatcaac tccatttcat ataaattttg tagattaatt
    55621 tacatctaca attttattac ttattcaaat ataacatgac ataaatttta taagttatgg
    55681 tacataaagt tcaacaaaaa tttcacaata cattaaaatg tgagtgttat acttaaattt
    55741 atatatttgt tttaattata ttcatttaat atttataatt tgaatttttt tttattaata
    55801 tttcattttt tagttgattg tttaatatat ttcatttttt atagtaattt tatatataac
    55861 tacgaaatat aaagtaatta gataagtaac aagaaactaa catgtaatgt taggtactct
    55921 agatatgaaa tcttaaaact tttaaatatt gaaatatttc tttatatata ttattgtata
    55981 tttcttataa attattgtca atttattttt tcttaaaaat aaaataattg cataatttat
    56041 ttcaaattat gcaatttata aaatttattc ccaaaactct tttactgttt cgattatctc
    56101 actataaggc aataaagtcg cttcgattat tgatttattt gtcttttcta aataatactt
    56161 aatcatataa tatccacaag catatcctgc acaataaggc aaccctactg gaaaatatcc
    56221 ttgcatttta gctatatcat caccataaag ataagatgtt atattttgaa atccagtttc
    56281 ttttaaagca ctttttatta ttggctttat ataagtattt aatgtttcga tatctgttct
    56341 actgacccaa ggtcctaaca tttcctctcc aaacatccat gttgcaaaat tttctgcaag
    56401 accttcattt atcatcattt cttctaatgt tatattatta ttccactcta taaactgaaa
    56461 tctaatattg tgattacatt catgtgctat taatactggt aatctattga tagtatattc
    56521 attaggaacc aatgatagaa atatatatcc aggaatccca ccatcacccc agtatccatc
    56581 acttaatatt gtataaggac tatttggatt cgccaataat attgaatatt tatagtcctt
    56641 aatgtttaaa tcataccctt cttttataaa agcatttata gagttttcta tggtttcttt
    56701 gcaagtgccc caaattttat cagcagataa cacatttata ttcttttttt gcttttcatc
    56761 aatatcctta ggcgataaaa cccctaacat ttcacttgct ataattacat catatccacc
    56821 tttctgtcta gcaactattg gaacattcat acacttccac ttaaattcaa aaggcttcat
    56881 caattcatat ctgtatatat cttctttttt gtttattggt gcatccatta tcttcttgta
    56941 tattttatct gagcgaattt ctataatatt catatttacc atctccttta agacaaagta
    57001 tatacaatta cgtagcgtca atgtcaatat acatttttta taatgtcacc ttaatctttt
    57061 tattatattt tatcctgtaa accttccgta catataacat ttttttaaat taataaattt
    57121 gtataatata ataataaaaa attatatact tcaaggtttc cctatgatac aatttactaa
    57181 atcataattt agattatcag gtgcaactat aaattattct atatcaagga ggatatattg
    57241 gtcatgctaa ctccatattt aatatttaat ggtacttgtg aaaaagcatt taatttttat
    57301 gctgaggctt tcggaggagg aaaaactata tttgcgcgat tagacagcaa tccaaacaat
    57361 cctattatgc acgcaagtgt tactttcaca aaatacgaag gttgtataat gggtgcggat
    57421 acagacaagc ctgttgtaat ttctggcatg gcgatttgtg ttgttctacc atctcgagaa
    57481 gcgatagaag aaatatctgt aaaacttgcc gaaggtggta cacttgtaca agaattttta
    57541 ccacacccac caccacatca aaatgatggc gcagctgaag tacttgatag gtatgggtat
    57601 acttggtatt taagtacata gttaaaataa aaaaactaca tctaataatc ataatataga
    57661 acagataaat ttcaatttaa tagtgtagta aaaaagtcgt gtcctaagac tgtcacgact
    57721 tttatttgta taaaaattta agtaaatatt tttgcgaata agtgagaagt ataactttaa
    57781 attaatttca tatgtagtat tggaaatggg ttaccttgtc catctatttc agacctatca
    57841 aacactttaa atcctaagtg aatataaaat cctacagctt ttttattctg ctcatttaca
    57901 tcaacatatt ttacattgta tttatctata gcatatttta ttaatttttt accaatacca
    57961 tttcctctat atttatcttc tataaagagc atctcaattt tatctttatc tattcctata
    58021 aagcccttta ttatatcatt atcttttata caaactatat tttctacttc tttaagtcca
    58081 atttcaacta atggtatcaa agattcaata tctttttctg ttaaaaaatc atgcgttgct
    58141 cttactgatg attcccagac ttgcaacatt tcgtcatatt ctgttgaatt tatttgtttc
    58201 atttcaagaa ttattccaga tttcataaaa atttcctccc tatatcactt ttaattgcat
    58261 aacacttttt tatacaaaac ttagaaagga ctacgaaggt taaatatcat agtcctcatt
    58321 aagtttaaat attatctatt atatattata ctataatcta gcaaattcac tacaataatt
    58381 cacatcagct tatactaaag gtgcttctat ttgagtagta aaaaggaatt tatgacaatg
    58441 accatcatta acagtagttt ctccacatat taagtgaatg tgtttatcag ttccaggtat
    58501 tctaattgct ggccctgttg taacacatat cttatggaaa tggtcaagga aatctacatt
    58561 atcatttatt ttatgaacat gatttgtacc tccatttatt ggtatagctt caccagtaac
    58621 ccctgctgca cggtggttat gtctatcctc acattcttct gctaacttaa cactttctga
    58681 atattcatga acatgtgtca tttcctcata atcattatag cacttgttgc acttattgta
    58741 tttataatca tcatacacat aatctctttc acatttataa ttttgataac tcatttttat
    58801 atctctccct atattatatt aagaaattct tgtaaagaat ctctaatgta taatatgaat
    58861 taaagtcaaa aaagttcata ttttgtaaaa ttatgtatta atttaattat ttttcccagt
    58921 atttagggcg tattagtgaa gctggtaact tcgtactaga atctcctttt gcagtattta
    58981 cttgtaattg tgttaaaaat atactatttt caagattagc acctctaata tcacaatctc
    59041 ttaagtctgc tcctattaaa tttgccccac tcaaatctgt atttcttaaa tttgctgcta
    59101 ttagaaatct acctcttaaa tctgctccta ttagatttat ttttctaagg tctgctccta
    59161 ttaaatctat atttttactt aattttttca tgtttttaag agtagaagtc ttgataaatg
    59221 atttaacctt tcccatcaca gactcactcg cttgtgataa taataaatta acctttgttc
    59281 tatgtgatat gatatccaaa ttaattatct gttctgggcc catattagtt atcttttcag
    59341 tttcttcaat tattaaatca atttttgatt tgattgtatc tgatatattt aatacagatg
    59401 cttctttaag ataccataac atttcatgta attgtctcat aattaaaaac acttcagaca
    59461 tttgatttgt tagttctgat gattgcatcc agtctatccc cttatatgtt acttgtgcta
    59521 ctttttgacc tgaaccaaaa caatcatatg cagtacaccc tttaaaacct cgcttcataa
    59581 gactattatg tacagaacat ttaaaatctg gttgtaaatt aatacagggt tttcctgatt
    59641 ccttatcgat aggaaatcca tctgaagcag aaaaataaag agctacacag caaagtccaa
    59701 agcacttctt acagtcaatc tttagctcct caaataactt attatcatat atatttttat
    59761 gtatatctat catatttttt ctcctcttaa gctttatttt taatattata ttatctataa
    59821 ctaaatgata atcaagtccc ttatttaaca gttcttattc ttaattataa tttaaggtat
    59881 tatttattcc aatagcatac ataagtttct ttatacttgt aacaatacct ttatactaag
    59941 acttatttta aatgtagcac tgtaataata aattgtttat atactcataa tgtatttgat
    60001 aaattgtaaa tctttataga agttgaaatt tttaatgatt ttagaatcaa gatataataa
    60061 taaatacaat ttataaaatc agaaatatat aaattaacta tacaaaccta aataacatag
    60121 catttaaatt tacaatttca gtataaatag tacaaagtat attagcttaa ataagattgg
    60181 cttatttgta gaggagagaa tattttggtg ttcaataaaa tttatattta ataaaaccat
    60241 aagaaaatat aaagataaat aaacaaactt ttattactta atagattctt atggttttat
    60301 actgtactga aaaacaataa acactacagc ttatttataa tattacgctt ttttaacaac
    60361 agaagacttt agcttcatag ctccaaaacc atctatctta cattctatat catgtccatc
    60421 tgatgagtca tggattaaac ggatattttt cacttttgtt ccaatcttta tagatgatga
    60481 acttcctttt actttaaggt ctctaattac tgtaacagaa tctccatcat ttaatacatt
    60541 tccatttaca tctttgataa tatttgaatc ttcttcagct cccaatccta atgcccactc
    60601 atgagagcat tctggacaaa ctaaaagggt tccatcttca taagtatatt ctgaattaca
    60661 tttcgggcaa tttggtaaat tttccatctt tatatgtcct ccatttgtta tttttagatt
    60721 tcttattata taaaatttct tttgagcctt tatgatatca tattatactt atatttacaa
    60781 actactaatt tacttaatgt ttaaattcta ttcgtattat tattaaactt gatttagctg
    60841 taatctcttt cttatattta ttatagttta tattattaca attttataac tctatctgcc
    60901 tgctccgata actctaaatc atgagttaca aatacgatag ttttaccatt cttatttaga
    60961 cttttaagta tatcaaatac tatgtctcta ttttttttat ctaaattacc tgtaggttca
    61021 tctgcaaata ttacttcaca ttcttttaat attactcttg ctaatgctac cctttgttgt
    61081 tctcctccac tcaattcgta tatctttttt ttattgtatt ttctaagacc tacagactca
    61141 agtgcgtaat ttattttttc attatggttg atattttttt gatactttaa agcaattttc
    61201 aagttatttt caacattttc attctcaatt aaagcataat tttgaaatag atagccataa
    61261 aaatcccttt gtaaaattct tatctgttta ctatttggat tttttatatt atttattata
    61321 atatcaccag aattaggttt ttctaataat cccattatat taagtaaagt tgtcttacct
    61381 gaaccactat ttcctgttat cgctacaaac tcgcccttag aaatttccag gttaaatttg
    61441 tctattatag atttatcttt aaatttttta gatatattta ttaatgaaat catcttttta
    61501 atgttctcct tttataattt tattaaatga actatttgag ataatcttgt ctaatacaat
    61561 agatacaatt acttctataa ctaatattag aagtccaaat aagataatat tattacttaa
    61621 atctactctt gttgatataa tgcctattgg aataatgttt gttaatatta atgagactat
    61681 taacaacata cttctagctg tagttgaata accaaatatt tttttaatat ataacttata
    61741 tttatttctt tcatagtagc tagctactat attgtaggta atcattaagt ttgatattgc
    61801 tattattatt atagtaaaaa tttcactatt caaatatttt tctagtttat ttatttctaa
    61861 accatactca ttataaacag catgtaaact ttggatatgc gcttctacac cttgagcttc
    61921 aataatgtta cttatatctg caagggcatc taatttctta gaattaaaat atacacatct
    61981 agatatataa gaaagataaa aagaatcatc aatattatct atgtcaacta ttgctattgg
    62041 gtcatcaatg agattcctat tgtcatccat aacaagggag ttataagtaa aatagctttg
    62101 attattatct acatatatta tattaacatt taaatctgac ttctttgttt tatttaactc
    62161 tctatttaat tctttattgt acatattttc tacttctatt ttttcaaaat aaaataattc
    62221 tctaaactct tttttaatct cattttcatg tacttgtaac tttttaggaa ctaataaatt
    62281 tatagtctta tcgtcataat cgattagatt aaatatttct ttgccatttg agtaaatagg
    62341 gttaacttta agatagtttt tatttatttt tatattttta ccatattgac ttacttctgg
    62401 gtttttaccc tttgaattta tttcatacat gtaacttcca tcttcaagtt tttcaaaatt
    62461 agttgaatct attaagaaag catctttttc ttctaccaaa tttttataga aacctttaat
    62521 cttggcattt ttaattactt cttcctttcc taaagattgt tcgcctgtat ctttaagagt
    62581 aagattataa acattttttg ctttatccca ctcagataga tttcctaact gagtttgtag
    62641 catattataa tttaaaatac agttatttat ggataatata agaaatgata caaatacaat
    62701 tttcaatgtg aaatgcatta tatttacaaa accataagat ttttttccat ttagagatag
    62761 cacatattta gaacttctac tattaaaacc aactattaat atagatatca aaatatttat
    62821 caaaatacat ataatcgaaa acatagcaaa gtataagcat gctctaacac ctatatttaa
    62881 ttttaaaata tagattatat atattaataa tgctgtgaca gatgataaaa tcataattct
    62941 aagtatattt tttataactc tagcaattat atctagctta gagtatccat ttaatcttaa
    63001 aatagaagcc tctctactca cactaattga ataatgtatt attgtagcca agaaacaaag
    63061 gataattagt attaaatctc taattattga aggatttaag tataaatttg agttaacata
    63121 tctatcatgt aatcttgcta caaaaatact tctgtctaca tttagttctc ttaatatttt
    63181 atttattgta tcttcatttt gagttgaaat atataagatt cctgattctc caacttcact
    63241 tattttacca aaatccctaa ttaaaattga tatgtcttta ctcatagttg agaatttacc
    63301 tacttgatta ctattattag tgtt
//
LOCUS       DAEMHW010000002        56841 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEMHW010000002 DAEMHW010000000
VERSION     DAEMHW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56841
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.002"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            95..973
                     /gene="purR"
                     /locus_tag="KPK67_000060"
     CDS             95..973
                     /gene="purR"
                     /locus_tag="KPK67_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBG2457087.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            1129..1407
                     /gene="spoVG"
                     /locus_tag="KPK67_000061"
     CDS             1129..1407
                     /gene="spoVG"
                     /locus_tag="KPK67_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBG2457088.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            1620..2999
                     /gene="glmU"
                     /locus_tag="KPK67_000062"
     CDS             1620..2999
                     /gene="glmU"
                     /locus_tag="KPK67_000062"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBG2457089.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            3094..4044
                     /locus_tag="KPK67_000063"
     CDS             3094..4044
                     /locus_tag="KPK67_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBG2457090.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      4884..4969
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            5131..5651
                     /locus_tag="KPK67_000064"
                     /pseudo
     CDS             5131..5651
                     /locus_tag="KPK67_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            5802..7478
                     /locus_tag="KPK67_000065"
     CDS             5802..7478
                     /locus_tag="KPK67_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG2457091.1"
                     /translation="MDKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFATGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            7490..8698
                     /locus_tag="KPK67_000066"
     CDS             7490..8698
                     /locus_tag="KPK67_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG2457092.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            8713..10425
                     /locus_tag="KPK67_000067"
     CDS             8713..10425
                     /locus_tag="KPK67_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBG2457093.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            10418..11326
                     /gene="pilO"
                     /locus_tag="KPK67_000068"
     CDS             10418..11326
                     /gene="pilO"
                     /locus_tag="KPK67_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBG2457094.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            11353..11919
                     /locus_tag="KPK67_000069"
     CDS             11353..11919
                     /locus_tag="KPK67_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG2457095.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            11956..12483
                     /locus_tag="KPK67_000070"
     CDS             11956..12483
                     /locus_tag="KPK67_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG2457096.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            12504..14042
                     /locus_tag="KPK67_000071"
     CDS             12504..14042
                     /locus_tag="KPK67_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457097.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLEKLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKIN"
     gene            14142..15224
                     /locus_tag="KPK67_000072"
     CDS             14142..15224
                     /locus_tag="KPK67_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBG2457098.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            15205..15987
                     /locus_tag="KPK67_000073"
     CDS             15205..15987
                     /locus_tag="KPK67_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG2457099.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVISYLMSGRKCRYCYEKISSRYAIVELITGILFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDNILIFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            16089..16739
                     /locus_tag="KPK67_000074"
     CDS             16089..16739
                     /locus_tag="KPK67_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG2457100.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            16753..17313
                     /locus_tag="KPK67_000075"
     CDS             16753..17313
                     /locus_tag="KPK67_000075"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBG2457101.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAAYAIDSIIRENIDMSMNKYNG"
     gene            17330..20716
                     /gene="mfd"
                     /locus_tag="KPK67_000076"
     CDS             17330..20716
                     /gene="mfd"
                     /locus_tag="KPK67_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBG2457102.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGAENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            20747..21742
                     /locus_tag="KPK67_000077"
     CDS             20747..21742
                     /locus_tag="KPK67_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2457103.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            21866..22405
                     /gene="spoVT"
                     /locus_tag="KPK67_000078"
     CDS             21866..22405
                     /gene="spoVT"
                     /locus_tag="KPK67_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBG2457104.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            22525..24138
                     /locus_tag="KPK67_000079"
     CDS             22525..24138
                     /locus_tag="KPK67_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG2457105.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIIPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            24245..25693
                     /gene="mazG"
                     /locus_tag="KPK67_000080"
     CDS             24245..25693
                     /gene="mazG"
                     /locus_tag="KPK67_000080"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBG2457106.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGVKLDMS
                     KFEKTWEELKKEEKGESYITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            25782..26060
                     /locus_tag="KPK67_000081"
     CDS             25782..26060
                     /locus_tag="KPK67_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBG2457107.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            26132..26374
                     /locus_tag="KPK67_000082"
     CDS             26132..26374
                     /locus_tag="KPK67_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBG2457108.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            26436..26690
                     /locus_tag="KPK67_000083"
     CDS             26436..26690
                     /locus_tag="KPK67_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBG2457109.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            26691..27224
                     /locus_tag="KPK67_000084"
     CDS             26691..27224
                     /locus_tag="KPK67_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457110.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            27230..27514
                     /locus_tag="KPK67_000085"
     CDS             27230..27514
                     /locus_tag="KPK67_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBG2457111.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            27627..28547
                     /locus_tag="KPK67_000086"
     CDS             27627..28547
                     /locus_tag="KPK67_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBG2457112.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            28637..31009
                     /gene="spoIIE"
                     /locus_tag="KPK67_000087"
     CDS             28637..31009
                     /gene="spoIIE"
                     /locus_tag="KPK67_000087"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBG2457113.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            31360..33252
                     /gene="pepF"
                     /locus_tag="KPK67_000088"
     CDS             31360..33252
                     /gene="pepF"
                     /locus_tag="KPK67_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG2457114.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            33366..34331
                     /gene="galU"
                     /locus_tag="KPK67_000089"
     CDS             33366..34331
                     /gene="galU"
                     /locus_tag="KPK67_000089"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG2457115.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            34831..36435
                     /gene="rho"
                     /locus_tag="KPK67_000090"
     CDS             34831..36435
                     /gene="rho"
                     /locus_tag="KPK67_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBG2457116.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            36588..36788
                     /gene="rpmE"
                     /locus_tag="KPK67_000091"
     CDS             36588..36788
                     /gene="rpmE"
                     /locus_tag="KPK67_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBG2457117.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            36933..37826
                     /locus_tag="KPK67_000092"
     CDS             36933..37826
                     /locus_tag="KPK67_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBG2457118.1"
                     /translation="MAKQAIGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            37844..38692
                     /gene="prmC"
                     /locus_tag="KPK67_000093"
     CDS             37844..38692
                     /gene="prmC"
                     /locus_tag="KPK67_000093"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBG2457119.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            38736..39800
                     /gene="prfA"
                     /locus_tag="KPK67_000094"
     CDS             38736..39800
                     /gene="prfA"
                     /locus_tag="KPK67_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBG2457120.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            39929..40609
                     /locus_tag="KPK67_000095"
     CDS             39929..40609
                     /locus_tag="KPK67_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBG2457121.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            40638..41678
                     /locus_tag="KPK67_000096"
     CDS             40638..41678
                     /locus_tag="KPK67_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBG2457122.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            41695..42144
                     /locus_tag="KPK67_000097"
     CDS             41695..42144
                     /locus_tag="KPK67_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBG2457123.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            42161..42613
                     /gene="rpiB"
                     /locus_tag="KPK67_000098"
     CDS             42161..42613
                     /gene="rpiB"
                     /locus_tag="KPK67_000098"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG2457124.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            42649..43278
                     /gene="upp"
                     /locus_tag="KPK67_000099"
     CDS             42649..43278
                     /gene="upp"
                     /locus_tag="KPK67_000099"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBG2457125.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            43579..44394
                     /locus_tag="KPK67_000100"
     CDS             43579..44394
                     /locus_tag="KPK67_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBG2457126.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            44620..45057
                     /locus_tag="KPK67_000101"
     CDS             44620..45057
                     /locus_tag="KPK67_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG2457127.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            45242..45469
                     /locus_tag="KPK67_000102"
     CDS             45242..45469
                     /locus_tag="KPK67_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBG2457128.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            45474..45860
                     /locus_tag="KPK67_000103"
     CDS             45474..45860
                     /locus_tag="KPK67_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBG2457129.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            45863..46567
                     /gene="atpB"
                     /locus_tag="KPK67_000104"
     CDS             45863..46567
                     /gene="atpB"
                     /locus_tag="KPK67_000104"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBG2457130.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            46636..46896
                     /gene="atpE"
                     /locus_tag="KPK67_000105"
     CDS             46636..46896
                     /gene="atpE"
                     /locus_tag="KPK67_000105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBG2457131.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            47019..47516
                     /gene="atpF"
                     /locus_tag="KPK67_000106"
     CDS             47019..47516
                     /gene="atpF"
                     /locus_tag="KPK67_000106"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBG2457132.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            47513..48058
                     /locus_tag="KPK67_000107"
     CDS             47513..48058
                     /locus_tag="KPK67_000107"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBG2457133.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            48075..49577
                     /locus_tag="KPK67_000108"
     CDS             48075..49577
                     /locus_tag="KPK67_000108"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBG2457134.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQSIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            49605..50483
                     /gene="atpG"
                     /locus_tag="KPK67_000109"
     CDS             49605..50483
                     /gene="atpG"
                     /locus_tag="KPK67_000109"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBG2457135.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLTGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            50496..51890
                     /gene="atpD"
                     /locus_tag="KPK67_000110"
     CDS             50496..51890
                     /gene="atpD"
                     /locus_tag="KPK67_000110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBG2457136.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            51893..52153
                     /gene="atpC"
                     /locus_tag="KPK67_000111"
     CDS             51893..52153
                     /gene="atpC"
                     /locus_tag="KPK67_000111"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBG2457137.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            52615..52995
                     /locus_tag="KPK67_000112"
     CDS             52615..52995
                     /locus_tag="KPK67_000112"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBG2457138.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            53010..53471
                     /locus_tag="KPK67_000113"
     CDS             53010..53471
                     /locus_tag="KPK67_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG2457139.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            53492..54082
                     /locus_tag="KPK67_000114"
     CDS             53492..54082
                     /locus_tag="KPK67_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457140.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            54097..55254
                     /gene="alr"
                     /locus_tag="KPK67_000115"
     CDS             54097..55254
                     /gene="alr"
                     /locus_tag="KPK67_000115"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG2457141.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFNVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            55287..55574
                     /locus_tag="KPK67_000116"
     CDS             55287..55574
                     /locus_tag="KPK67_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBG2457142.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            55543..55926
                     /locus_tag="KPK67_000117"
     CDS             55543..55926
                     /locus_tag="KPK67_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBG2457143.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            56097..56351
                     /locus_tag="KPK67_000118"
                     /pseudo
     CDS             56097..56351
                     /locus_tag="KPK67_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
BASE COUNT        23047 a         6049 c        10600 g        17145 t
ORIGIN      
        1 ataataaaac ataagtaata ttataccaca ataagaattg ttatgcatta gttagtttaa
       61 cgttatataa aggtacttgt aggaggaaat aataatgaaa tttaaaagaa cagagagaat
      121 tggagcaata gttaaaatac tgtctgataa tccaaataaa atatatactt taagttattt
      181 tacaaatcaa tttaatgcag caaaatcaac aataagtgaa gatttattag ttgtaaaaaa
      241 tgtatttgag aagctacatt taggtaaagt tataactatt tcaggagcag caggaggagt
      301 aaaatatata cctaaaactt ctatagcaga aaatgcagag ttcttaatgg aattatgcga
      361 gagaatatgt gacaagtcta gaatactatc tggtggtttt ttatacttga ttgacctaat
      421 ttacgaccct acaatagctg ctaagatagg aaagatattc gcatcaaata ttgagtatgt
      481 agatgcagat tatgttgtga caatggaaac aaaaggtata cctatggcac ttatgactgc
      541 aaaagctatg aatttacctc tagttataat aagaaaagat ataaaggtat cagaaggtcc
      601 tacacttagc atgacatatg tgagtggaaa cagttctaaa gttgaaagta tgagtttgcc
      661 aagaaaagct cttaaaccag atagtaaagt tataattata gatgatttca tgaggggggg
      721 aggtactata aaaggaatgg tagaccttat gaatgaattt ggtgcagagg ttataggtac
      781 tggagtattc atatctacaa cgaatccttc agaaaaaatg gtaaaagatt atatttcatt
      841 gattcaattg gatgttaatg gagataaaat agtagtagaa ccaaacttaa agacttttaa
      901 agatgaatat agaaatgaag atttagacga agaagaagat tcagaatttg aatttgagat
      961 agatgaagat taataaaaaa aatatttaga aaaatatgta aaaataaaaa gaggatatcc
     1021 ctagttgttc atagaattat ttaaaaaaca gtttatatta atttattcca gcgataataa
     1081 ttttaatata aattaatttc tgtcaaaatc aaagggggat attttggtat gaagataact
     1141 gacgtaagag taagaaaatt aacagaagaa ggtaagatga aatgtatagt ttcaataact
     1201 ttcgataatt tgtttgtagt gcatgatata aaagttatag aaggccataa tgggctattt
     1261 atagcaatgc ctagcagaaa agtaggcgaa ggaaatttca gagatatagc tcatccaata
     1321 aatgcagaaa tgagacaagt tttagaagat gcagttttac aagcgtatca tgaagcatta
     1381 gttcaatggg aagtagctgc tgaataataa taaatatata aaatggagag agcccgaaca
     1441 tttaggctct ttttaaatgt agaatttcta ttaaaatatg tactttttag taaaaaagtg
     1501 ctatattatt agtgataatg tatatgttgt atacaaattg ttagataaat gtattataat
     1561 gtatattgat taagagaata atacgaataa aattaaatta gattgaatgg agtgtatcaa
     1621 tgaactttaa agctataatc cttgctgctg gcaaaggaac aagaatgaag tctaaatacc
     1681 caaaagttat acataaagta tgtggaaaag aaatggttaa tcatattata gatgtttcaa
     1741 aaaaatcagg ggtaaaagat actgtcgtga ttttaggaca tgaagctgat gtagttaaag
     1801 aaaagcttgc agaagaaatt ataatagcca tgcaaacaga acaacttggg acaggacatg
     1861 ctgtaaagat ggctaaagaa tatattaatg atgaagatac aatagttgta ctttgtggag
     1921 atactccact tataaaagaa gagacactaa agagactatt tgaatatcat atagaaaata
     1981 aatatcatgc tacagttctt acaacaagag ttggaaatcc aacaggttat ggtagaataa
     2041 taagagataa gaagggtgac cttcttaaaa tagtggaaca aaaagatgca aattcagaag
     2101 aaaagatgat aagtgagata aactctggaa tttattgttt taatggaaag agtcttagag
     2161 aagcactaga tttgctaaat aataacaatt cacaaggtga atattattta actgatactg
     2221 ctaaaataat gagagataaa ggattaaaag ttggtgcatt tgcaggttca actattgaag
     2281 aacttatggg cgttaactca agagtagaat tatcaaaggc agaagaaatt atgagacgaa
     2341 gaataaatga atctcatatg gtaaatggag taactataat agacactaac tctacctata
     2401 tagaatctga tgttatgata ggcaatgata ctataatata tccaggtgtt atgttacaag
     2461 gaaaaacaag gataggttca gattgtataa ttggaatgaa ttcatctata acaaattcag
     2521 aaataggtga tggtacagaa ataaaaaatt caactataat tgatagtaag gttggagaaa
     2581 attctactgt tgggccatat gcatacttaa gaccaaagag tgacttagga aataatgtaa
     2641 aaataggcga ttttgtagaa gtaaaaaatg ctattataga agatggctca aaagcatctc
     2701 acctttctta tataggagat gcacatgtcg gtaaaaatgt aaatattgga tgtggagtag
     2761 tatttgtaaa ctatgatggt aaaaataaat ttaaatcaat agtaaaagac aatgcgttta
     2821 taggttcaaa ttctaattta gttgcaccag tagttgtaga agaaaaagga tatatagcaa
     2881 caggttcaac aataacacat gatgtcccag atggtgcttt ggcaatagca agagaaagac
     2941 aagttatcaa agaaggatgg gtagaaaaaa agaatcaaaa agacgaccaa agtaagtaat
     3001 tatgtagttt attattttgg ctaaaaaatt tagctcgaaa ttattataaa ttatattcat
     3061 tagtatttta aaattttcag gagggttaca taaatgaata ctagcggaag cgagattaaa
     3121 attattgcag gtaattcatc gaaagagtta gcacaaaaaa tagctgacta tataggtgta
     3181 tcagtactag attgtgaagt aggtacattt agtgatggtg aaatatgtgt aaacatgaat
     3241 gagacagtaa gaggttgtga tgtgtttgta gtgcaatcta ctaacagccc agtaaatgat
     3301 aacttaatgg agttattaat cttaattgat gcgttaaaga gagcatcagc aggtagaata
     3361 acagcagtta ttccttacta tggatatgca agacaagaca gaaaggctaa ggcaagagat
     3421 ccaatcactg ctaaattagt tgcaaattta ataactgctg ctggagcaga tagagtatta
     3481 acaatggatt tacatgcagc tcaaatacaa ggatattttg atataccatt agaccactta
     3541 ctaggtggaa caatattagc aaattatttt aatgaaaaga aaatagaaga tttagtagta
     3601 gtatctcctg acttaggaag tgttactaga tcaagaaaat ttgctaatac tttaaatgga
     3661 gaagttccaa tagctataat agataaaaga agaccgaaag ctaatgtatg cgaagttatg
     3721 aacttaatag gtgatgttaa aggtaaaaat gttatactat tagacgatat gatagataca
     3781 gcaggaacaa tagttaatgc tgcaaatgca cttaaagagt ttggagcaaa agatgtttat
     3841 gcttgttgta ctcatggtgt gttatcaggt ccagctattg aaagaattgc taattcagaa
     3901 ataagtgagt taatagttct tgatactata caacttcctg aagagaaaag aatagataag
     3961 ataaagataa aaacagtagc tcctttattt ggagatgcta taagaatgat attctctaat
     4021 gaatcagtaa gtaaattatt ctaatttatt gtatttatga gagttgttta aaaggatata
     4081 aaccttttga gcaactcttt tttgatttta tacttatgga atgttatttt tttcttgtta
     4141 gaaagatata tattattgta aaaagagatt atagtttaaa attatgtata tttaagtaaa
     4201 ataaacctaa taaaggatat attattcaaa atattatgaa tataaaaaat attacaaaaa
     4261 agtattgaaa aaattcagat tatgtagtat tatgtaaata taaataaaga aagaggtgtt
     4321 tccaatggga aggttagaag atgaacccct aataattaaa tgattcagtc acaatctaaa
     4381 gtgaatataa aaatctattg aaagtttatt tataagaatt gaaaatagta tctattctat
     4441 ctttagtgtt tgtgagaggg ttctggatta tataagatta ctgtaaagag taattttgca
     4501 caataagcat atctgtaaat gaagagtttc gtttatataa tgattgtggc tgaatcataa
     4561 ctataataga tagaatgttt ataatagatg attttataaa atactatatt gtaatagctt
     4621 ttagctttca aataaatatt agcaaaggat ggaaatgaca atgataaaac taaatgataa
     4681 tttataaata tctccttacg atatataata attgtaggga gatgtttctt tttttttgta
     4741 tagcataatt tttctagaaa ttctgtttca atatgtaaaa agttggtata tataaatagt
     4801 ataaacagtg ttgctaaaat atggtagata ttatataata taagtggata tgtatataca
     4861 ttatgagtta tataatttga atttaataaa atagaaacgt tgatttatgt tctgtaatgt
     4921 gggcaccttg gagcatattg agttagtggt gcaaccggct atgaaattgt atttatttat
     4981 aaatactatt attttcatag ctttttttat ttaaaaaatg gttatatgtt agaaataaat
     5041 ttggttagta cgtttaaata attttactta gatttttaac cagatatatt ataagtaagt
     5101 attaattaaa aaatttaagg gggaataaaa atgaagttaa aaaagaataa aaaaggtttc
     5161 actttagtgg aattattggt agtaattgca attataggta tattagcagt agtggcagtt
     5221 ccagctttat ttagtaatat aaacaaggct aaggtagcaa gtgttgagtc tgattatagt
     5281 tcagttaaga gtgcagcatt gtcttattat tcagatacta ataagatacc agttacacca
     5341 gatggtcaaa ctggtttaag tgttttagaa acttatatgg agtctcttcc tgataaagct
     5401 gatatagggg agaatataaa ttgattaaag ttggtagtaa attggtatta cagataggta
     5461 caaatactga gggagttacc ttaacagaag cacaatcagc aaaattattg agtgatatag
     5521 gtgaaaataa aatatataca agcgctacaa caaatagttt gggaaatcca ttaacaagta
     5581 atacaaaaat agataataac gttctatata tagtacttat agataatact gtgatggaca
     5641 caacaaaata gttaaaacaa tatttttata aaaatattgt aaatttaatg aaaaatataa
     5701 tataaaagac aggaagaaaa tcttcctgtt tttttatact attttcttta atgttcaatt
     5761 atgaatgatg atataattga gatagtaaag gagtgaaacc tgtggataaa aaagttagaa
     5821 taggagacaa actggtcgaa aaaggatata taacagaaga acagcttaaa tgggcactat
     5881 cagaacaaaa aaacagtgga aaaagactag gagaatttct agtacaagaa gggcttatag
     5941 atagtaactt actaataagt gtattaaaag aactcttgga tatagaaagt atatttttag
     6001 aagggacaga aatagacact ttagctacaa aaatggttcc agaaaatata tgcaaaagat
     6061 atactgtatt tccatttaaa attgatggaa ataaaatatg tctagccatg tcagacccac
     6121 aagacagaga agcagttcaa gatgtaaggc gtatgtcagg taaagacgta gagatattta
     6181 tatcaagtac agaagatata aacaaagcaa taggtcatgc ttatgcacat tcagaaataa
     6241 ataaagcaat gactgagtac aacaaaaata gaactggtgg agtaagagaa acagtaatac
     6301 ttgaagaaga tgtaaatgct gctccaatag tacgattagt aaataacata ctagaaaatg
     6361 ctgttagaat ggaagcaagt gatatacata tagaacaaag tgaaaattac atgcgtgtta
     6421 ggtttaggat agatggaatg ttaagagaat atatgaggat gaactcagca ccatataagg
     6481 cagtaatcag ccgtatcaag ataatgtcag atataaatat atctgaaaag agaataccac
     6541 aggatggtag aatctattta aaagtagata ataaacccat agattttagg gtttctacaa
     6601 tgccaactaa cagggatgaa aagatagcta tgagggttct tgataagagt aattttatgg
     6661 ttagtaaaga agtactagga atagacgaac atggctctaa gatatatgat gaattgataa
     6721 acactcctta tggtcttata ttggttgttg gccctactgg aagtgggaaa accactacat
     6781 tgtattcaat gttaaatcaa ctaaatacag aaaatagaaa tctattaaca atagaagacc
     6841 ctatagaata tgaattacct ggtgtaaatc aatctcaaat aaatgaaaag gcagggctta
     6901 ctttcgccac cggacttaga gcttttatgc gtcaagaccc agatataata atggttggag
     6961 agataagaga tactgagaca gcagaaatag caattagagc ttctcttaca ggacatttag
     7021 ttttaagtac attacatgca aatacagcag ttggagcaat ttcacgtctt ttagatatgg
     7081 atgtagaaag ttttttaata acttcatctg ttttaggtgt aatatctcaa agacttacac
     7141 gaaaaatatg tgagcattgt aaggtttcat atgaagcaga tattggagaa aaaaaggctc
     7201 ttggtattga tgtaaatgaa tcagtaacta tttatagggg aaaaggttgt gaaaggtgta
     7261 ataatactgg atataaagga cgtctaggta tatttgaaat gcttgaaatt actccagaaa
     7321 taaaagagtt aatagattca tctgcaaatc aaagagaaat tttaaagatg gctagaaaac
     7381 aaggtatggt tagcttaaaa gaggatatag ttaaaaaggt tttaaatgga aagacaacag
     7441 tagaagaaat gattagaata attttgatga ctgattaaag gatgatatta tgaaaagttt
     7501 taaatacaca gtaataaaag aggacggaaa aaagcaaact gatgtgattg aagccaacga
     7561 ttttaatgaa gctagaaatc ttcttagaaa gagaaatctt agagttattg aaatcaaaga
     7621 aagtaaaaat aaaaatataa gattatctag tagaaagaga aaaaaagact taggagctga
     7681 ccagataagc cacttttgta gacaatttgc tattatagta tcttctggta taaatagtat
     7741 ttctggatta gaaacattag ctagaaggtc aactaacatt acattaagag aagaaattaa
     7801 tcgtatagta gcagaaataa aagtaggttc tactattgca gattcaatgc tttcatcaaa
     7861 atcaaagttt ccaaagttac ttggtgctat ggtagctaca ggtgaagcta caggtaaatt
     7921 agatgaagta ttaaaaagta tggcttcatt ttatgcaagt gaacacaggg ttaaacaaaa
     7981 gcttagaaat gctgcaacat atccattaat agttttgatt tcttcattta taatgatttt
     8041 tatatttaca acatttatgg tgcctaagat gattaattca attacaacag taggagcaac
     8101 tcttccactt ataacaagaa ttgttatggg gtttggtatg tttatgaaga agttttggat
     8161 aatagtactt ttagttatta tattatttat ctatcagttt aaaaaatatt taaaaacgtc
     8221 agttggaagg gctcataaag atagagttat aaacaagata cctgttttag gaaaaggaat
     8281 aaattgtatg gtagctacac gattttcaag agcattgtat ctatttgtat caacaggata
     8341 cccattagtt caagggcttg attatattat agatagtgta aataacacca tggcagagaa
     8401 gattttagca tcagctaaag atggaataac aagaggagaa ggcttagcag aaaatttaga
     8461 gagatatacg tattttgact ctgtgttggt acagatgata gctatagggg agcagacagg
     8521 ggaattagag aatataagta gacagatggc agaattttat gagtatgaat ccgagattta
     8581 tttaaatagg atggcctcaa tgatagagcc tgttttaata attgcagttg gtattatagt
     8641 tgcgatactg gttgtatcaa tatttatgcc aatgcttagt atatatgatg ccatgtagga
     8701 gggaaaggac aaatgaacaa ggtttatgta tcatattatg gcgattctat ttccatagtt
     8761 gagggcagat ataaacacga taaggataag ttttatgtaa aaaattttaa tgtgttttca
     8821 atagaggata ttgcacaaga tttttctaag gaaaagtatg ctctattgag atatgcatta
     8881 gaaaatgtta agttaaaatc aagacatgta gttttttgtt taaatacaag agatgtaatt
     8941 ttaaaacccc aaaaacttcc taatatggac aaaaaggatt tagatggatt tatgaagcta
     9001 gagatggacg aaatgatggc tttggagtca gatgaatatg tattttcgta tgaagtatct
     9061 agtgaaacac ctgataattc agatggcgat gaatcaagct taaatcttat cattggagct
     9121 atacaaaagg aagaggtaaa tgcaattgta gagataatgc atgaatttga tttagtatta
     9181 gatagaatag atacattatc aacagcttac ttaagaatgc ttaaaaattt agactacaat
     9241 gatataatgg taaccaatat aagtgacaat agcacaatta taaatatata taagaaagat
     9301 actttattta ttagtgataa tatacctata aagataactg ctaaagatat ggacatgcaa
     9361 ttattaaatg tagtagaaga atcaaaaggt cttatgaatt actattcgtc aagaaacttt
     9421 ggtaaggtta cggatactat attaatatta ggaaaaagat attccaataa attaatttat
     9481 gaaactttta gctctgcatt ttcaagctat gtttctcaag gccttactga gataatggat
     9541 atatctgata tgataactgg agatatagat gaagatgata taaattcaat tgtagagctt
     9601 ttaggaagta tgatagaacc taaagggaaa agagattttg aaaatataaa ctttttacca
     9661 atggatatac ttagaaaaca gataaaagct aaacaaatga aaaacatact gaaaattgct
     9721 ccaatagtta ttttgattct tgctatacct tatttatgtt taattttttc aaataattta
     9781 gcaaataagg aacttgaaaa tgttaatggt gaaataagaa atattgaatc tgcatattat
     9841 cagattggaa atatagaaaa aatgatacag agtaaaagcg aagaaataaa ggtatatgat
     9901 atgttaattg ggaaaaaggt aaagtgggaa aatttattgg atgatataga taaaaatact
     9961 ccaagtaaag ttgaactaac aagtttatca acaacttatc aaaatcttgg aactaataat
    10021 aaagctgata attcaaaaag taataagact acaacagata aatcaagtaa caatgaaaca
    10081 aaatcaagta cgaaaagtga tagtaatgga gataaaacag ataatgattt aaatgaagat
    10141 aagactacaa atgtgtcaga agcattaact ggaaatagtg agactaatag taataacacg
    10201 gataaagatg ataaaactcc attgtatgat aagataccaa atgttataaa tatagaagga
    10261 aatgctaaag attcctctta tgtaggtcaa tttttatata aattaaaggg attatactat
    10321 ttcaaggatg ttaaattaca tagcataaaa agtaatgcag aagattctaa ctatacattt
    10381 agtatgacat tatacctgaa agaaggtgta ttaactaatg aataaagata tatttaaaaa
    10441 agatataaaa gatatattta aaatggaagt tagtctaaaa gggattctta taaaggttat
    10501 aattttatta gctattttat catatggtta ttttggactt taccctcaat atcagaaata
    10561 tattgctaca aaaaattctg taaaacaggc tactgaaaaa ttaaatatat ataaagagaa
    10621 aactgcactt atgccaaaat atgaaaaaca acttgaagaa gttgaaagtg aattaaaaga
    10681 aaaaagcaaa aatcttttat atgatatgca agatggtctt tttttaatag gtttagataa
    10741 aaaaatgaag gcttataata ttgacttaac cagttatgac gttggaaatg ttaaagaata
    10801 cgaacatttt tatggtatat caacaacaat aagtgttaga ggtgattata gaaatgtcaa
    10861 aaaattaatg gcttatttag aagaacaaaa aaatgttact caaatactag attatacaat
    10921 gacttcatat atagaaccag aaaaagaaga ggaagaaaca acaaacaata aggatttaat
    10981 taaaaatgtg aacataaata aagatacaaa aatatactgg gtggaaggcg aaaataagtg
    11041 gcatttatat aaggattgtc cattgataaa agagggagag attctaaaaa atgacaaact
    11101 tttagatagg tctactaatt atctatgtaa tacttgtgta cttagagctg caacagaaaa
    11161 agaagcaaaa aaagaagtcg tagctccaaa ggcagaaggt gtagtagatg ctacatttaa
    11221 atttataatg tacactaaag agaatactac aatgatactt gatacagata atccatcttc
    11281 atggagacca ggaaaatata atccatttaa gtctagtgcg aaataaagct aagtaagctt
    11341 taaagatagg tgatgagttt gtacaaaaat aatgaaaaag gtcttacttt attagaagta
    11401 ataatagctg tttttatact gacaatagtt ttaagtattt cttataaagt gttcaatggt
    11461 ataacatcag cagtaaaaaa gcaacagatt attacagatg ctcaagtaaa tattaatcta
    11521 attaataagt atctaaatag agatttggaa aactgtaaag agctaactaa aactggttca
    11581 ggtaataatt atgaatacaa tatagagatg ccagataatg ttgtaaaata tgaagtttct
    11641 atagaaacta aaaaaaatac agaggtatat tctgtaacga gaatccaaaa caatacaatt
    11701 gatacagaaa atgaagttag agaagagata atctataatc aaccactagt tcaaaacaat
    11761 aaagaaatga aggaaacacc atttaaaata gaaaagcaaa ctggtaaatc tatttatact
    11821 gtaagtatat actataatga atcagtgcaa gaaagtcata aaaatagcaa tttaaataac
    11881 aaaacttata catttgatgt tatgtcaaga atagggtaat aagaatttat taaaaaataa
    11941 gtaggttgat aaaatatgag taaaaagaat agtgaaagag gattttcctt aattgaagtg
    12001 ttagtagcta tggctattat gggaattgta ctatttgcat tttttaatat tatcaatact
    12061 aataacaaag caaacactaa aaatgacaca gatataactt ccttaaacta tgtccaaagt
    12121 gaaatagaaa atctaagaga aaagataaaa agtggagaat ttgattttga tagtttagat
    12181 aaactagaag atggaactgt cgtatatgaa aaattaatag ataaatcaaa aaaagttgta
    12241 tatgataaag ttcttagtga aggtgacgtg agcttatatg atactccata tgaaaagatt
    12301 acaacaataa aagatgaaga tggtaattta atcgacaaag gaaatattac aaataagata
    12361 aagactattg tagaagataa aagtggtcag atatataaaa tagctgtaac tgggaaaagc
    12421 atgaatgact attcaagtaa aaaagaggtt aaaattgtaa ctgaaatatt taaagataaa
    12481 taattttact tgggggtgag attttgagga agtggaataa atttaaaagt gaaagagggg
    12541 cagctttagt tttagttttg gttgtagtag cattgctttc tattgttgga ttgatatttt
    12601 caaatcagat agctaataga ataaaatcta caaagactac taatgaaggt atacaagcaa
    12661 agtatttagc agagacatgt gtggaaaata gtattgataa agcatatgaa aaattgtatg
    12721 atgagcttga gaaaatggat aatgagttta aaagtgaaaa tcaagaaaaa tccatatcta
    12781 ggtcaaaatt aagaaatata tcagatgaag attttaataa tcaagatgag gaaaatattg
    12841 aagcagaaag acttggatat atgaataata taaatttcta tttaaataaa gcttcttcag
    12901 acttggaaaa agctagtatg gaattgaaaa agctatatga tttagatatg ttggactata
    12961 gagacattga atatgtagat gcaaatatta tttcacatag agattctatc ttagaaattt
    13021 gcaagaatta tacttctgga gatattagta aaataaatga atatattctg aaagaagata
    13081 tagactcaac tacattgatt gaggcaaagt tagtgaataa tgatattctt ttgaaaatgt
    13141 ttttagaaga aaataaaatt gaaaatgaac atttaaattc agccttttca catacttata
    13201 aggcattaga taacatttcc ttggcgatgc aaaatatgat tgagtataga catacgtttc
    13261 atatagatga gccaaaagta gaagtatcta atggaattcc agactctcaa caatattatg
    13321 aactaataca aaatcctata ataaatagta tggagtatag ctggaatagt aaatgggata
    13381 cattagaaaa actattggaa attttaccaa atcaaactca aggatttaat tctttaagag
    13441 ttcatttgag aaataatgta agaaaatttg agaagttaag cgataatata tctagtggta
    13501 aaaaaaatac agcaaaaaat ttcttaaagt ataaagagtt attgtatgaa atatcagacc
    13561 aatgtaatca actaaaaagt atgtcttatg agaaaatacc tgttaaatat gataacatgg
    13621 ctttgataac aacttttgac tatatacaaa atgagctttt agcagagata aaatgcagat
    13681 taaaagaatt aaaaccacaa gaaattgata aaacagaagg tattactata aaaatacctt
    13741 tttataaggc tgactacgac atgacaaaag aaggatggcc taaattaaaa gaaaatggtt
    13801 caggggcgga gttaagctta atggtaactg gtgataaaga tggaataaaa gaagtagaag
    13861 ttacagatgg taaaaaaaat attattggtt taggtgttga agaaaattca aattcaaagt
    13921 ataaggttga cgccattgta aactttaatc taaatataga tacaaatgta gttggaaatt
    13981 atgatataaa agataaaatt ttaattaatc atgatataag ttcatacaaa aaaataaact
    14041 aaatataaga agcaacttag tataaataaa taataaaata attttattat ttactactaa
    14101 aaaaataagt aaactaatag aagatatgga gagtaaatat tatgtatatt tatgatttat
    14161 tagagcaagg tataagacta aacgcatcag atatacacat aacagttgga acaaatcctg
    14221 tagctagagt aaaaggtgga tttgtaaaac taagtgaaca aatattgaca tcagaagtaa
    14281 ccatgcaaat ggcaaaagac atagcggggg aatctatgtt taaagtaata gaagaacatg
    14341 gagaagctga tttttcagct tctctaaaaa caggagaaag atttagggta aatgcatata
    14401 ggcaaaaagg aaattacgca atagcaataa ggactataac agcagaaatt ccaacatttg
    14461 aaaaattagg ccttccagag agtataaaat cttttactga aaagcataaa ggattagttt
    14521 tagttacagg tccaacagga agtggtaaaa gtacaacact tgcaagtatg ataaatataa
    14581 taaatgaaaa acaacaaaaa catataatta cacttgaaga cccgatagaa tatgtacatc
    14641 atcataaaca aagtttagta aatcaaagag aagtaggaac tgatacagaa agttttcatt
    14701 cagcccttag agctattttg cgtcaggacc cagatgtaat actaatagga gagatgagag
    14761 accctgaaac tgtttcaata gcattaacag cagcagaaac aggtcattta gtattttcaa
    14821 cactacatac agtaggggca gctaagacaa ttgatagaat agtagacatg tttcaaccat
    14881 cccaacagca acaaataaaa acacagttat ctacagtttg tgaaggtgtg gtatcacagc
    14941 agttacttcc tacagcagat gggaaaggca gaatagcagc aattgaattg atgtttgcta
    15001 ctccagcaat aaaaaacctg ataagggaag gcaaaacata tcaaattcca aacatgatac
    15061 aaactggagt taaagtaggt atgaaaacta tggaccaaga tttaatggaa ctttataaaa
    15121 atggaaagat tacaaaagat atggcactta gtaggtgtac tgaccaagag tttatgacaa
    15181 gaatgatagg tggagtaaac tacaatggat attataatcg ttaatctata tgtatttata
    15241 gtgggtatag tgtttggaag tttctttaac gtctgtattt ttaggattcc agaaggtata
    15301 tctatttcta gacctccttc tcattgtatg agttgtaata ctagactaaa gccaatagac
    15361 ttaataccag tgataagcta tttaatgagt ggcagaaagt gtagatattg ttatgagaaa
    15421 atttctagta gatatgctat agtggaatta attacaggaa ttttattttt aacagtattt
    15481 atgttatatg gaatatctac atcaactatt tattatttgg ttcttgtgtc tttactgatt
    15541 ataattacat ttatagatat tgaccacttt ataataccag ataacatatt gatttttgga
    15601 gctatatttt ctatgatttt taacctttta tttaaagaaa ttccagttaa ggattcaatt
    15661 ctaggtgttt ttatttgtgg aggaagtgtc tggattatag ttcttttgat tgaatttgta
    15721 ataaaaaaag agtgtatggg tggaggagat atcaaactat ttgcaatgct aggcctttac
    15781 atgggtgtta aaaatggtct tttgacagcc ttactaagcg tttatgtagg agcagtctat
    15841 ggaatctgtg taataattat aagtagaata aaaggaaagg aatataattc tgttatacca
    15901 tatggacctt ttatatctat tggagcatta ataacaatct tatgtggaaa gcaattactt
    15961 gaactttatt tttctatggt tatatagatg atagtcaagt taaataaagt tttaatttaa
    16021 aaaaatgaat taaagtattt aataaggaca atattattat taaccctatt actaaagtgc
    16081 tacaaactat ggagtatata ataataattt ccttattttt tgcacatttg ataaataaat
    16141 ctctatgtgt tttttgtgat agagaaaaga aaaatttata caatacgttt tttatttaca
    16201 taataactgt agttttatct gtattaatat atctaaaatt tgggattagt gtagaatgtg
    16261 tacaatatat ggttttaatt ccatttttaa taattatatc cattgtagat tactacacaa
    16321 tatatgtata tgatataaca atagttagtg gtataatagt tcaaggtata atttttttgg
    16381 tctttttcaa tatagttcaa tttgattata taaatcatat atttggacta ttgataggct
    16441 ttattatacc ttatattatt gctaaactaa caaaaggatt aggctatgga gatgtaggtc
    16501 tctatagctt atgttgcttt gcattgggaa ataattatgg gttttatgtg atagttttat
    16561 catttatttt agcgtttttg ttttttatta ttttatacat aactaaaata tttaaagatg
    16621 tagatataag aaagcaaatt ccttttacac cttttatttc cttgtcaaca attttaataa
    16681 tatttacaga gtataatata tttaattttt attataattt gttatacaaa tttatataac
    16741 aggaggtaag aaatgtacgt agtagtaggg ttaggtaatc caggaaagaa gtatgagaag
    16801 actagacata acgttggatt tgatgttata gatattcttg ctaaagaata caatattagt
    16861 gttactaaaa taaagcataa agctttgatt ggtgaaggga gagtaggtac tgaaaaagta
    16921 ttattagtta aaccacaaac ttatatgaat ttaagtggag aaacattaat agatatatat
    16981 aaatattata aggttgattt aagtaatata gtagtagtat atgatgatat agatttagaa
    17041 gttggaaaga tacgtataag aaaaaaagga agtgggggaa ctcataatgg catgaaatct
    17101 ataactaagt gcctaggttc aaatgacttt ccaagagtga gagtaggtgt atctaaacca
    17161 gaagctggtc aagacttagc agactttgta ctttctagat ttagaaaaga agaatctgat
    17221 aacataaatg aagcattaga aaaagcggca tatgctattg actcaataat aagagaaaat
    17281 atagatatgt ctatgaacaa atacaatgga tagtgagagg gagatttaaa tgaatgatgt
    17341 ttttttatat cctttgcaaa attcaaaaga gtataaagat ataataaact gtataaaaaa
    17401 tacaaaaggc tcattattgg ttaatggact tttaccagta caaaaacctc atatatcgta
    17461 ttctatattt aatgatttat cacgacaaat gatttttata acgagtagtg atttagaagc
    17521 aaagaaagta tacgaagatt taagttttta tatggaagat aaagttgaat atttaggttt
    17581 tcaagatatt tatttttatc acttggatgc aaaggacaga aacgaagaag cgaagaaatt
    17641 aaaagtattg ttaaaactag caaataaaga aaaaatcatt ttagtaactt ctattgaagc
    17701 agttttaaga aaatacatac caaaacaagt tttgctcgat agtgtatctc attataaggt
    17761 gggagatagc ttagacttag aaaagttaac tgagaaatta gtttcattgg gatatgaaag
    17821 agtttcaaaa atagaggggt ttggtcaatt tagtataaga ggtggaatta ttgatgtatt
    17881 ttcacttgaa tatacaaatc caattcgtat ggaattattt gatgatgaga tagattccat
    17941 acgaactttt gacgtatatt cacaaaaatc aattgataaa ttgcaacaat tctctataac
    18001 tccatctagg gaatttattt atcccgaaaa aactacagat gcattagtta aattaaaaaa
    18061 agaaacaact aaaaatactg atgaaggtac atttcaaaat atagattata tatcaagtaa
    18121 gacttatttt gaaggtgcag agaactatat agattatatt tatccagaag aaaacaaaag
    18181 tatatttact tatttagcag atgatgcaat agtatttatt aatgatataa cgagacttaa
    18241 agaaagatgc gaaaattata taaatgagtt tagagaaaat tataaattaa atcttgaaag
    18301 aggtctggct attaaaaacc aaggtaaatt gttatatcac tatacagatt tagagtactt
    18361 agtaaaagac aagtctgtgg tattaaactc gctattgcca aagtctataa ataacttttc
    18421 tataaaatct ataataaatt ttgaaagtag agaagttcct acatttaatg gaaaggtaga
    18481 tttgcttgta gaagaattaa atcggctgaa atataatgga tacaaaataa ttttagcaac
    18541 aaatacctta gaaagagcaa ataaattagg aaaagattta ttggacaaag gcttagaaac
    18601 gaccatttct aaagatagag atattgaaat taaatcttca caagttataa ttgtacctgc
    18661 acacataaac agtggttttc agtataaatc tataaagttt gttgttataa cagataacga
    18721 aatgataggt gtttataaaa gagcgtctaa gactagcaat aaaaaagtta aaaagggaaa
    18781 gaaaatagaa tcctttttag atttaagtgt tggagactat gtagttcatg aaaatagtgg
    18841 tgtaggtaga tacacaggga ttgaacaaat tacagtcaat gctattaaaa aagattatat
    18901 gaaaatagta tatcaaggtg gagataatct atatgtgcca atagaccaga tggacaaagt
    18961 acaaaaatat attggtgcag aagtagaaaa agttaagtta aataagcttg gaacaaatga
    19021 gtggacgaaa gcaaaagcaa aagttaaaaa agaaattgaa gatatgacaa aagacttgat
    19081 tgagctttat gcaaaacgtg aaaaaataca agggtataag ttttcaaaag atacaccttg
    19141 gcaggcagag tttgaatcgt tgtttccata tcaagaaaca gaagaccaat taaaggctat
    19201 agaagaaact aaaaaagata tggaatcaag taaggtaatg gataggctag tatgtggaga
    19261 tgttggttat ggaaaaacag aggtagctat tagaagtatt tttaaagctt gtatggacca
    19321 aaaacaagtt gctgtattag ttccaacaac aatattggcc caacaacatt ataatacatt
    19381 caaagagagg tttgaaaatt atcctttgag agtagaagtt ttaagtaggt ttaaaacacc
    19441 aaaacaacaa aaacagatta ttgaagatgc taaaaaagga ttagtagata ttttaattgg
    19501 aacacataga ataatatcca aagatataaa tttaccaaac ttaggtcttg tagtcattga
    19561 tgaagaacaa agatttggag taaaacataa agaagcttta aagaaaatta aatctacagt
    19621 agatgtactt acactttcag caactcctat accaagaaca ttacatatgt ctttaagtgg
    19681 aataagagac atgagtgtta tagaggaacc tccacaagaa agacatcctg taataacata
    19741 tgttacagag agtaaggaaa gcgtaattca agatgagata gagagagaat taagtagagg
    19801 tggacaggta ttttttgtat acaatagagt tgaacacatt gaagaaatgg caagtatgat
    19861 tcaaaaactc gtaccagatg ctagagtggc tgttgcacat ggaagaatga cttctaaatc
    19921 attagaaaat ataatcctag gatttttaaa taaagattat gatgtactag tctgtactac
    19981 aataattgaa actggtatgg acatatcaaa tgcaaataca atgattatat atgatgcaga
    20041 taaaatggga ttggctcaac tttaccaatt gagaggtagg gttggaagaa gttcaagaca
    20101 aggatatgca tatcttttgt atgaaaaaga taaaacttta agtgagatag cggagaagag
    20161 attaaaagct atcagagaat ttacagagtt tggttctggt tttaaaatag caatgagaga
    20221 ccttgaaata agaggagcag gaaatatttt aggttctcaa caacatggtc atatggcagt
    20281 tataggttat gatttgtacg ttaaaatgct taatgatgca attaaaaaag tcaaaggtga
    20341 acctattgtg gaggaaattg atgttgaaat agatttaagt gtaaatgcat acattccaga
    20401 taattatatc aaagatgagt tgattaaaat tgaaatgtat aagaaaatag cttctattga
    20461 aaacaaagaa gacatgttgg atatacaaga agaacttgag gatagattct ctgatatacc
    20521 taaaccagtc cagacacttc ttacaatagc ttatataaaa tcgttatgta agatattaaa
    20581 aatagaaaag ataagacaat taaaagatga aatattatta gttccaataa caaagtatag
    20641 aactaaacag aaaataggtt ataatatagt tacagaatta gaagaattat tagaaaagat
    20701 gtgcaaggta aaataacttg taaatttaag aagggatgtt ttaaatttga aaaaagtaat
    20761 aacattggta atagctatga ttttggtagt atctgtgact gcttgcagtt ctagtaaagg
    20821 agaaactgtg gcaacagttg aaggtacaaa gatatcatca gatgaattta aaaaaactat
    20881 agctctttat aaagattcta tggaacaaac ttatggaaaa gatatatggg ataaggaagt
    20941 tgaaaagggt gtaaaataca aggacaagtt taaagattta attcttgacc aacttataac
    21001 tacagaagta atatattctc aagctaaaaa agataactta cttcctaaaa aagaggatgt
    21061 tgaaaaaagt tttaaagaat taaaagacgc tatgggtaaa gatgaaaaat ataaagaaca
    21121 acttaagaaa ttaggtatag atgatgagtt tttaagagac caacaagaga aagatttagc
    21181 aatgcaaaac tatcaaagta actttgctaa aaagactaaa atatcagatg aagaaatgaa
    21241 aaaatactat gatacacata aagatgaatt taaaaaagat gaggtagaag cttctcatat
    21301 tttattgaaa acagtagatg ataataacaa gccactttca gataaggaaa aagctgaagc
    21361 taagaaaaag gctgaagagg ctcttaaaga agttaaatca ggtgaggatt ttgcaaaagt
    21421 agcaaaaaaa tattcacaag atacttcagc aagtgatggt ggtaaattag gattcttttc
    21481 aaggggtcaa atggttgctg aatttgaaga tgctgctttt tctatgaaaa agggtgaagt
    21541 atctgattta gttgaaactc aatatggata ccacataatt aaagtgacag acagaataaa
    21601 tgagcaaaca tcatttgaag atgctaaaga aactataaaa gaccaactgt tgaaaaataa
    21661 atatcaagaa caaatagaaa aattaactaa agaagctaag gtagaaaaag atgaaaaagt
    21721 aataaataaa atcacaatct aagacttaaa aaaagagtaa tatgcttaaa aaagctatat
    21781 ctggataaga tatagctttt tttgtatatt tttcttaaca atggtaaaaa taaacttaaa
    21841 gtaatttatg gagggattaa aaaatatgaa agcaacaggt atagttagaa gaatagatga
    21901 tcttggaagg gtagttatcc caaaagagat aagaaagaca ttaagaatta gagaaggaga
    21961 ccctttagag atattcacag caaaagatgg agaagtcata cttaaaaaat actctccaat
    22021 aggtgaatta aatgaatttt cgcaagaata tgctgaaaca ttagctgaag tactaggata
    22081 tggagtgttg ataacagact tagattctat aatagctgta tctaagcttc ctaaaaaaga
    22141 ttacaaagaa aaaagtataa gtgatgagtt agaagcaata ataagtgaaa gaacagtaaa
    22201 atattcaaaa gataaaaaca ttttaccatt attcaaggaa gaccaaaaag aatatactag
    22261 tcaagttatc atgcctataa ttagtgcatc tggtgactgt ataggttcaa ttgtagttgt
    22321 ttcaaaggat aaagatacaa ttgcagaaag tgaagaaaaa tctttaaaaa ttgctgctaa
    22381 ctttttagga aaacaagttc aataatttta tcataaagac cttttacatg aaggtctttt
    22441 gaagtttttg tgtaaaatat tgaatatagc attaaaaata tggtatatta aagtttgtta
    22501 tataaacagt atggaggtta tgttatgaac aataacaaaa ataaagactc ctttctaaaa
    22561 ggagccctta tacttgggct agcaggtgta atcgtaaaaa taatgggtgg attttttagg
    22621 attccccttg gaaacatgat tggttcagaa ggaatgggat actatcaggc tgcttatcct
    22681 gtgtatactc tatttttaac attagctact gctggatttc caacagctgt ggcaaaattg
    22741 gtttcggaaa aagttgcaat tggaaatttt aaaggtgcaa ataagatatt taaggtatca
    22801 catacagttt tattcattac cggtataata tcattttgta tattattttt tggagccgac
    22861 tacatagtaa caaacgtaat gaaaaatcca ggtgcgttat actctatgaa agccattgct
    22921 ccagcattac tatttgttcc tgcaatgtct gcatatagag gatattttca agggagacaa
    22981 gatatgacta agattgctgt gtctcaagtt gcagagcaat tttttagagt tgtattaggt
    23041 cttactttgg cttacttgct tatgaagagt ctaggtcaga cttttggtgc agcaggtgct
    23101 atatcagggg ctacaatagg ttcaatagca tctatgcttt atttggtgtt tgcttatatg
    23161 ctagggagaa aagagcgtag agcagagata gatgcaagcc aaagatttaa agatgaaaga
    23221 gtgtcttata tttttaaaaa gcttttaact gttgccattc ctataacaat aggagcatca
    23281 gtaatgccac ttgtaaatat gatagataat gttattgtaa taagaagact tatggaagca
    23341 ggatttacat acaaagtggc aaattcaatg tttggtcaat taacaggtat ggcaatggcc
    23401 acaataaatt taccagcagt cataacaact gcaatgagta tgagcttagt tccagcaatt
    23461 tctaaagcat atgcattagg aaataagtca aaagctagaa aagatactaa gagtgcagtt
    23521 aaggtaactt tacttatagt attgccttgt gcgtttggta tggcttcttt agcaatacca
    23581 ataatgggtc ttttatttcc acatgaacca tcttctgtag gaactatttt gtttactctt
    23641 actccatgtg tattattttt aggattaata cagacattga ctggtataat tcagggaatg
    23701 ggaaaaccaa taatacctgt aattgcttta tgtgttggta tgctatgtaa aattgtaata
    23761 agttatactt taacaggtat accagatatt aatgtactgg gttcagcatt tggtacagtt
    23821 acagcatact ttgtggcagc catgattaac ttactatatg ttaaaaaaca catgaatgta
    23881 aacttttcta aaaaagaatt tataataaag ccatttataa cagttatgac aatgtttata
    23941 atggttaagt tgagttatgg tgctttagtt ggattcttag gaaacagcat atctacaata
    24001 attgcaatat gtgttggtgg aattgtatat gtagtagtaa tacttggaat aggtggaatt
    24061 aaaaaagaag agattctaac tatgccaaaa ggtgataaat tatataaatt gttaaaaaaa
    24121 gttaaactta taagataata taggaatata tatgcaaata aaggaaatac tatacaaata
    24181 gtaaatctcc ttatttgtat aataatataa aaataaagtg aaatttgcta ataggagtga
    24241 tgttatgggt aagataagta ttgtaggtct tggaccagga gactattctc ttataagcca
    24301 aggtgtgtta gaatctttaa taaatagccc taaagttttt tttagaacag agaaacatcc
    24361 aataattgat aaacttaaag aaactataca atacacttct ttagactact tttatgaaaa
    24421 agaggaaaat tttgataatg tttatttgaa aatatctgaa tttatcatag aagaatcagc
    24481 aaagggtgac ctagtgtatg ctgttcctgg tcatccaaga gttgcagaga agactgtaag
    24541 tataatagag agattagcca atgaaaagca catagcagta gaaatattgc cttctatgag
    24601 ctttgtagac gctatgttta actatttggc tatagaccca tctgagggat ttaaactggt
    24661 agatgctttt gaaatagaaa attcatatat agacttggat acaagtatga taataactca
    24721 ggtatatgat aaatttatag catctaatgt aaagttaaaa cttatggaac attatgacta
    24781 taatcaagaa gtatgtatag taaatggagc aggtgtcaaa gatttagaaa gtaaaaagtt
    24841 tataaagctt tgtgaattag acagaaatga aaactttttt gactatttaa ctagcttata
    24901 tatacccaaa agttctaaaa agatgtataa tacagtacat gacttagaaa taatagttaa
    24961 tacacttaga agtccatctg gatgtgaatg ggataaaaaa caaacacatc aatcattaaa
    25021 aaattctgtt atagaggaag catatgaact ttgtaatgca attgataata atgatataga
    25081 tgaaatggta gaagagttag gtgatgttct tttacaggtt atattccatt gtcagatagg
    25141 aaatgaggag ggctactttg atttaaaaga agtagtaaat ggcatatgta aaaaattaat
    25201 tcatagacat cctcatatat ttagtggtgt aaaattagat atgtctaagt ttgagaaaac
    25261 ttgggaagaa ttaaagaaag aagagaaagg cgaaagttat ataacagatg gccttagaag
    25321 gataccaaaa catttaccag ctcttatgaa ggctagtaaa gttcaacata aagcagcact
    25381 tgtcggcttt gattgggaca atgtagaaga tgtatttaaa aaaattgaag aagaatatca
    25441 ggaactactt gacgaacaca gggtcgggaa tataaaatac ataaaggaag agctaggcga
    25501 tttattattt tcgatagtaa atctagctag atttttagac atagactcag gagaagcctt
    25561 gaactgtact acagaaaaat tcataaatag atttgacttt atagaaaaaa gtgcaatgaa
    25621 cttgaataaa aaattagaag acatgacact tgaagagatg gatgagtttt ggaatcaggc
    25681 taaaaaacaa taaaattgtg gaaggaaatt tgtcaaaaat ctagaacttt tatcacaaag
    25741 atagaagtct atattataaa catttttagg aggtaaatat cgtgaataaa gctgaattag
    25801 tatcaaagat ggcagaaaaa agtggattaa caaagaagga agcagaagct gcgttaaacg
    25861 catttatgag ttctgttcaa gatgcactag taaataatga aaaagttcaa ttagttggat
    25921 ttggaacatt tgagacaaga gaaagagctg ctagacaagg aagaaatcca agagatccag
    25981 agcaagttat agatatacca gcttctaaag caccagtttt caaagctgga aaaggattaa
    26041 aggatataat aaatggataa ttagaaatct tgtgaaaaag tgtggataaa tttgccacac
    26101 tttttctata gaaccaagga aggtgataat tatgagatta gataaatttt taaaagtatc
    26161 taggattata aaaagaagaa ctgttgctaa agatgcatgt gataaaggga ttgtcactat
    26221 aaatggaaaa gttgctaaat cttcttcaga ggtgaatatt ggagatacat tagaaataca
    26281 atttggagag aagaagatga agtttaagat aaatgaagta aaagagcatg tattaaaaaa
    26341 tgatgcaaaa gaaatgtacg aaattttaga ataaaattaa tagtcctcta gtatattaat
    26401 agtagtatta ttgaaatatt tactgggggg atattatgga acagaacata actttgaaag
    26461 atagatcaaa gctggttatt tcgggagtcg agcacatata ctcttttaat gacaaaagag
    26521 tagagctcaa aacttctgta ggcgagatgg taattgaagg cgaaaaccta gatatgagca
    26581 agctaagtat agacgaaaat ataattagca tagatggaac tataaattct atggtatatg
    26641 caaagcctaa aaagccacaa gagagtttct tgaaaaaggt atttagatag atgatacctt
    26701 ttactcaaga tgtaagcatt ttttatgcta ctatttatgg tggaatactc ataggggtgt
    26761 tgtttgattt ttatagaggg ctaagaggga attttaaatt catcaattat tttgctataa
    26821 tctttgatgt attgttttgg tttctagcta cggttataat attcgtaaca ataaacctta
    26881 cagaattttt tgatttaaga tattatcatt ttgtggcttt gtttattggt tttattttat
    26941 actataacac cataagtaaa attgttttat caattatcaa taaaataatt cgttttgtga
    27001 gaaattcttt taaaaaagtt acacattaca tagtcagttt tttaaataat ttgtattacg
    27061 ttataatcta ttcactacac ctactatttg atattatatt ttatatacca aacatattta
    27121 tagcaacaag aaaaagtata aaaagaaaat caaataaaaa attaaagaac aagaaaaaat
    27181 caaaacctaa gaagaaaaaa aataaaacta aaaaaagggt gtaggaaaaa tgaacttaag
    27241 aaaaaaattt tcaggacaga ttattgttat cagtttattt ttaggaatat caatttttag
    27301 tatgatgaca ggatttgtat ttgagtatac aaaagctaag gaatataaaa aagaaatagc
    27361 aagtctaaat aaacaattaa aaaagactga aatacaaata aacagcttaa agaaagatga
    27421 aaaatcatat gaaggagact tagaggatat agcaagaaaa agattgaata tggtaaaacc
    27481 aaatgaaaca gtttatgtag acataaatcg ttgattaaaa cttgctaata agtttacaga
    27541 tgtattgatt ttaaaattta agatttaata aaaaagtcat tcagttaagg atggcttttt
    27601 gtttttattg aggagggaat ttcataatga aagtaggagc tatagatatt gggacaaact
    27661 caatgagatt attgatagca gattatagag atgggaattt tataaacaga aaaaaatttg
    27721 ttaatacaac tagaattggt tatggtgtgg atagtaaagg atatataaaa gaagatgcta
    27781 tcaaaagaaa tataaaagca ttaaaagaat tttctgataa gtgtaatgat gaaaagtgtg
    27841 aaaaaattta ttgtatgggg acatctgctc ttagagacag taaaaatgga gattactttg
    27901 ttaaattagc taaagaagaa actggaatag atgttgatat agttacggga atagaagaat
    27961 caaatttggg ctttaagggt gttttagaag gaatacaagg tgatgatact attattgtta
    28021 tagatattgg aggaggttca acagaattta ttgtaggtga ttcagaagga attaagttta
    28081 ataaaagtga gaatgtaggt gctcttagga tgacagagag gtttttgaag cacgatatta
    28141 ttgaagatga agagtttgaa gctatgagtt catttatata tggtgagatt gacaaaacgt
    28201 taaatcatat aaaagacaga ggattaaaaa ctcttatagg tataggaggt acgattacat
    28261 cattatcagc aatgaatcaa gaattagaga tttactctat ggaaaaaata cataatagta
    28321 aaatacacaa aaaagaaata gaagaaattc tacaaaaatt aataaagatg acattaagtg
    28381 ataaaaagaa cttaaaaggg ctacaagaaa aacgtgcgga tattataact gctggtgtaa
    28441 aaatattgaa tataataatg gaaaaattag aaatagaaga aataaccgta agtgaatacg
    28501 ataatttgga aggcttaata tgtcaaaagt caaaaaaaat gtattaatat tttttgattt
    28561 ttaaaaccga ttatgacaaa aaaaattact cctctttgtt acaattctta atataaacaa
    28621 aagaggaggt ttttttatgc aaagaagtgt agcagttgtg aagaataaga ctgtaaaacc
    28681 aaatgtcata aaattaataa gcggatatat tgattttact acggcggcat tcgctgtgat
    28741 gggattttta cttagtaggt ctattatagt agactctgta gctcctttag gaattgcctt
    28801 ttttatatgt gcatcaaaga ttgataagta caagatacct gtattcatat caacattatt
    28861 aggaatactg ttatcatcta atgattttgg aaatgtagca aaatatgctg tgtgccttac
    28921 aatatttatg gctatgaata aaaaattaaa attatcagat tcaattgcta aggttgcatt
    28981 tataggtgca ataatactac ttccaatctc catgggacaa gctttctttt caaataggta
    29041 tgtatatggt cttcttatag caggagtaga agcaataata atgtttatat ctatatatgt
    29101 gttttcttat gggataggtt taatggccaa tgtcaacaat aggatgtctg tacggacaga
    29161 agaagctata tcaataagtt tactgctaac ctttagcata atgggaatag gagatatatc
    29221 tctgtttgga atatcaataa ggaccatttt atcgaccgta ttgatattgc tgacctcaat
    29281 agtaggtgga gctaccatgg gagcatctag tggtgtaatt attggtgtag catccatttt
    29341 aaataatatg acatcagctg tttatatggg gatatattct ttttcaggat taataagtgg
    29401 agcatttaat aaaataaata aatatttctg tatactagga tatattctaa gttggacaat
    29461 aatataccta tatacttctg gtattacttc taatatgatg caattaagag atatactatt
    29521 aggttgtttg atagtactag tattgccaga gcgattattt aataagattg aaaaattaat
    29581 aaaaagtaat gtggcttcaa atgaaatcgt atacgattat attatgagaa gcaaaaacct
    29641 aactaatagc agattgaaca gtatttataa aacttatgat gaccttgcag atacatttga
    29701 taagataaga gaaaaagaca aagtgctaga ccaaagagac atagctaatg taatagatat
    29761 gattcataat gatgaatgta aaagttgtag tatgagaaga atgtgctggg agtcaagatt
    29821 taatcataca tacactatga tatataacat tcttgaaaaa attgaggaga aaggggagtt
    29881 aagtctaaac gatataccta aaaattttag aaaggaatgt atgaaaccag agtcaatagt
    29941 gaaaatatca aatcactatt acaaaatgtt tgtattagat tatgattgga gtgtgaagtt
    30001 ttcagaaagt agaaagttaa tagcaaatca gataagaagt atttcaaaat ccattaaatc
    30061 cttatctcaa gatttagaag gcgacataat gctagatata gaaaaggaaa aaaatatata
    30121 tgaacagtta gaaagatatg atataacagt tgataaagta agttacttaa caaaaagtaa
    30181 cagtgagttt gaaatatcaa tagaaaagaa aacttgccat gatggatgta tgtgtgaaga
    30241 taaaattgta aatataattt cagacctagt aggtgaaaat atgtctgtta gaaaaatagg
    30301 atgtcactgt ttaggaggta aatgcaaggc aacattcgtt aaatctcaaa aatataaggc
    30361 tgttacagaa gtttcagcaa tgtcaaggga tgggcatatc ctttgtggag acaattacac
    30421 atacatggag ataaatgatg gtaaatacat gatggctata agtgatggta tgggaaaggg
    30481 taaaaaagca tatgaagaat cttctgccac tattgatata ctggaaaaaa tgatagatgc
    30541 aaaaattaaa gatgagatag tcattgatac tataaataat atgttgttgt taaaatcttc
    30601 agaagagatg ttttcaactc tggatttggg aattttagat ttaaaacgag gttgtttaga
    30661 aactataaag atgggagcgt gttctactta tataaaaaga gaggatggag aagttgactt
    30721 aatatcatca tcatcattac ctgtgggtat attatcagat gtaaaaattg atagaaaaaa
    30781 tgtaaaagtt aaggaaggag actatgttat aatggtttct gatggaattg tagatgcagg
    30841 aagaaataac aatttaggag acaattggtt aatatacttc ttaaagaata tagagactac
    30901 aaatccaaag gaaatttcaa atttaatatt ggatagagca ttagagttac aagcgttaca
    30961 aatcgaagat gacatgactg ttttagttac gaaaatatgt acaaaataag gtttacaaga
    31021 ttatgtgtat aagatgtgga taacttgtgt ataatatgtt aatagacttt tgaaaaaagt
    31081 atagttatta acaaaaataa catataaatc aacaaaattt gttgataagt atggcagaaa
    31141 tgttaataaa atgtaaaaaa agaaaaaaca aaagctatta tcaaaagaga ttcatgttga
    31201 aataactaaa tttctaagta aagaaaacct gtgaatatgt tgattattat gtggataact
    31261 attaattcga tatttgttta tatagtatga ataattgaat aatatatcta aaaaaagcca
    31321 tcctattatt aagtaaactt attgaaagga tggcttttta tgaatagaaa attgtgcaaa
    31381 gcaataatac ctttatttat ttctattttt tgcatgttaa taataagtat aaaagctgac
    31441 caaggatatc aaaagaataa tgacagagca attactgtca tgaataataa tgatactgaa
    31501 gatgatgctg aaaaaaaaca agaaatagac tatgaaaaaa catgttggaa tttgcaatct
    31561 ttgtttaaaa gtgatgacca atggaaaaaa gaacttaaga gttttgataa agatactaaa
    31621 gagttgaaaa attacatagg taaagtaaca aaatcgccaa cacatttgtc ttttgcctta
    31681 aacattaaag aaaaacttga tataagacta aataaacttt ctgcctatgt aaaattaaaa
    31741 caagatacta ataaaaattc gtataagtac ttagatatga atgatagtat gagtaaatcc
    31801 tatgggaatt atatggggat ttgttcagat ttagagttag aaatactaaa attatcaaac
    31861 aaagattaca agaaaattat ttcaaataaa aatataaata ggaaatatgg tgcatattta
    31921 agagatataa gaaggaataa agtacattat ttggatgata aatcagaaga tattctaagc
    31981 aaagtatctt caataagtag tcttccaagc caagtatatg aattatttag aaatatggat
    32041 agaaaaacta atttgacacc agctcaatat gcgagtgaat tagagtcatc agatagagaa
    32101 aatagaaaaa aagcttatca agatgaactt ataacttaca atgataatat aaatacaatt
    32161 gcaggtttaa ttactggtca agttaaaaag aatatttttt attctcagga aagaggttat
    32221 gagtcttctt tagaaatgta cctggaatct gataatgtag atgaaaaagt atacaataat
    32281 ttgataagga ctgtaaatag taatatggac agtctacaca aatatataga tttaagaaaa
    32341 aaagtattaa aattagataa ggttcattca tatgatatgt ttgtgcctat ggttaaggct
    32401 gttgataata atattaatta tgaaaaggca caaagtatag tatattcagc gttgtcacct
    32461 ttaggaaaag aatacaatga tgtagtatat aaagctttca atgaaaaatg gatagatgtt
    32521 tattcacatg aaaataaagt aagtggtgga tactgcttat ctgtttatga aaatcatcca
    32581 tatatacttt taaattataa taattcatta ggttcagttt cgactttatc acatgagctt
    32641 ggacatgctg tatatgaata tttaagttct aagaatcaga attattttaa ttcaaatccg
    32701 tctatattta ctcacgaagt tgcatctaca acgaatgaag cattacttta cgagatgctt
    32761 ataaaagaag ctaaaggcaa tgatgaaaaa gcttattata taacacagta tttagacttg
    32821 ataaaagata ctctttatac acaaactatg tatgctgaat ttgaaaaaac tatacacgag
    32881 ttagtagaaa aaggaaaaag tgtaaacgca ttagtactaa atgatgtttg gggacaactt
    32941 cttaaaaaat attatggaca aagctatgaa ttagaccaat tatctaaggt cggatgggct
    33001 agaatcccac atttttataa tagtttctat gtctataaat atgctacagg ttgtagtgca
    33061 ggagtaagtt ttgcccaaga catcctaaag aatggttcag ataactatat gagctttctt
    33121 aaaaagggaa gttcagatta tccaataaac ttgttaaaat caagtggcat aaatttaact
    33181 aatacaaaac ccatagaaaa tacaatcaaa aaatttgata gtttagtagc agaacttgaa
    33241 aagctattat aaacattatt gtaatccaca tggtaaatat atcgaattaa tgataaaata
    33301 tactaaaagt ttaaagtcct atcaagatag gactttttat ttattaatta gtgggagatg
    33361 attctatgca agttaaagtt aaaaaggctg taataccagc agcaggttta ggaactagat
    33421 ttttgccagc tacaaaggca caaccaaaag agatgttacc aatagttgat aagccaactc
    33481 tacaatacat aatagaagaa gctgtggcct caggaataga ggaaatacta ataataacag
    33541 gtagaaataa aaaatctatt gaagaccact ttgataaatc tgtagaattg gaacttgatt
    33601 tagagaaaaa aggtaagaaa gaattgttgg aggtagtaca aaacatatct aacatgatta
    33661 acatacatta tataagacaa aaagagccaa aagggttggg agatgcaatt tattgtgcaa
    33721 ggcattttat aggtgatgag ccgtttgcag taatgttagg ggatgatatt gtagataatg
    33781 atgtaccttg tctaaaacaa ctaacagatg catatgagga atatagaact acaatattgg
    33841 gtgttcaaaa ggtaaatcaa gaagatacta ataaatatgg gataatagaa gcaaaaaata
    33901 tagaaggtag agtttacaag gtaaaagaca tggtagaaaa gccagagtca ggaaaagctc
    33961 cttcaaatat tgctatttta ggtagatata taataactcc tgaaatcttt gatattctta
    34021 aggatttacc accaggtaaa ggtggagagg tacaacttac agatgcttta aaaatattat
    34081 ctaagaaaga agctatgtat gcatacaatt ttgaaggcaa gagatatgat gttggggaca
    34141 agctaggatt tttagaagct actgttgact ttgctctaaa gaaagaagac cttaaggaag
    34201 attttataaa atatttgaaa catgtatgta gtgaatttga taaaaataac aatgtgttat
    34261 acaataatac agatgaaaaa atagatttaa ttgaagtcaa tgaagctaga gaacttgaaa
    34321 ttcaaaaata attaatgaaa ggtgatgtat ggtggcaaaa tcaagtcaga aaagaattaa
    34381 tagaataata gctgttggga ttgtgggaat cctcgtaata acgagtataa cagctgtttt
    34441 gagtatgatt gcaactatgt aataagataa atgacagcag ttgatataca taaatataaa
    34501 aaccatatat aacttgatgt agtcaaaaac ttacaatatt ttagttatat atggttttta
    34561 gaatttataa atgtaaaaaa aaatttttat aatagttgca tattttaaag gaaaatatta
    34621 ttataagata taaatatagt gataaattta taaaaacgtg ctaagattgg acttgaaata
    34681 cagtaatata gtaggtaata atgttttaaa ttttatttat tgaccataat atataaataa
    34741 tgatattatt agacttaact gtttaaataa aacttttctt tttcatgcct tcaatttcca
    34801 aaataagtat gtatataaat gaatatatag atgaggagga tttttttgat tttagatgat
    34861 aaagtattag aaggattagg tagtaagacc ttagttgagc tacgagagat agctaaagaa
    34921 ttaaaaataa aatcaattac tacatataag aaaaatgagt taattgagat tataaattcc
    34981 aaaagtaaaa atgaggaaaa agtaaacgaa gagactaaag aaaaaaacga agatattaaa
    35041 gaagacataa agaatagaaa agaaaatata gagcgtgaga gtatagaaaa agaagttgaa
    35101 tataagagcc ctaccaaatc atacaacaag aaagaaatag atacccaaaa tatttcccaa
    35161 aaccaagaac atagaaatca aagaaatgac tataataaga caacgagtgt aaatgatagc
    35221 aataagtcat caaatatatc taatagaatg gtcagaaata ataataaaaa ctattatatg
    35281 cctaaacaag ttgatgaatc taagatagta gatgaattta acacatcaaa agaggatgaa
    35341 gtagtaggtg tacttgaaat tctgccagat ggatttggtt ttttaagagg ttcaaattat
    35401 ttgtctactg aaggagatgt ttacgtttca ccatctcaaa taagaagatt taatatgaaa
    35461 acaggagaca agattaaggg aataactaga catccaaaaa gtggagagaa atttagagca
    35521 cttttatatg ttcaaaaaat aaatgatgaa aaccctgaca cagctataca aagaaatgcg
    35581 tttgagacat taacaccaat atttccagaa gaacgactta cactggaaac taatagaaat
    35641 gaaatagcta caagaattat agatttaatt tcacctatag gaaaaggaca aagaggattg
    35701 atagtagcac ctccaaaagc tgggaaaaca gttcttttaa aaagcgttgc aaatagcata
    35761 gctaaaaatc atccaaatgt tgaacttata gtactcctta tagatgaaag accagaggaa
    35821 gttactgata tgaaagaatc tattgaagga gacgttatat attccacttt tgaccaagta
    35881 tcaagtcatc atgtcaaggt tgcagagatg gtgttaaata gagctcaaag attagttgaa
    35941 catggtaaag atgtagtaat tttactagat agtataacta gattggcaag ggcatataac
    36001 cttacaatat ctcctacagg tagaacttta tctggaggta ttgaccctgg tgcacttcat
    36061 ggacctaaaa agttctttgg tgcagcaagg aatattagac agggtggttc tttgacaata
    36121 ttaggaacag cattggttga aactggttct aggatggatg atgtaatatt tgaggaattt
    36181 aaaggtacag gaaatatgga gttacattta gatagaaaat tagcagaaaa aagaatattc
    36241 cctgcaatag atatttacaa gtctggtaca agaagagatg atttattgct tgatgatgaa
    36301 gaaaagactg ctctttggag attaagaaga gaaatgagta ataattcagt aatggaaatt
    36361 acagataaag tcattgaact tataaagaga actaaggaca ataaagagtt tgtaaaatca
    36421 ataaagaatt tgtaaaatca ataatacaaa tgcaaatatg gattatataa actaatagaa
    36481 aaatcacttg aatggcataa taagttgtgc tataataatg tagttgataa tgtaaaaaga
    36541 aagttaatat aattaatttt tttaaataaa gagaagaggt gactataatg caaaaggaaa
    36601 tacaaccaaa atacaatcca gttgaagtgc gttgtgcttg tgggaacaca tttgttgctg
    36661 gttcaactaa ggacgaaata aaagttgaaa tatgttcaga gtgccatcca ttctatacag
    36721 gaaaacaaaa gaacatagaa aaaggtggaa gaatcgacaa attcaagaaa agattcaaaa
    36781 tggactaatt tttataaaag tggagttgga ggaagccaac tcctatttta ttacaaaaaa
    36841 acattcatat tctactttaa attgtctttt tgtattataa taataaatat gtaaaattga
    36901 gtattctaaa tacaataaaa tgaggtgagt ggatggcaaa acaagctata ggtgggcaag
    36961 ctgttattga aggtgtaatg atgcagtcaa aagacaaaag agcggtagct gttagaaaaa
    37021 gtgacggaga aatagcacta aaagaagata gaataaaaag ttgggtaaga gataagaata
    37081 tagataagat accttttgtt agaggttctt ttgttatgat agatacaatg attcaaggca
    37141 taaaaagctt aaacttttct tcagagtttt ttatggaaga agctgaagag gataaatttg
    37201 atttatttat aaagaaaatt tttaaagata aggcaaatga tataattata atattttcat
    37261 tggtcatagc aatgttactt tctgctggat tattcatttt tatacctaca ttggtaggag
    37321 gagctttttc taaagtaatg cctaatgatt ttatgcttaa tttaatagaa ggaatcataa
    37381 ggatagctat attatttgca tatatagtat tgatttcacg aagtaaggat atagaaagag
    37441 tctttcaata tcatggagca gaacataaat ctatatattg ctatgaaaat gatttagaac
    37501 ttacagtaga gaatgctaga aaatttaaaa gattacatcc tagatgtggt acaaatttcc
    37561 tatttatagt tatggctgta tcaataatat tgtttgcatt ttttggttgg ccaaatccaa
    37621 ttcttagaat atttatgaga attatatgtg tgcctatagt agcaggttta tcttatgagg
    37681 taattagagt tcttggaaaa tatgataatg gatttactaa aattattgca tatccaggaa
    37741 tgatgttgca atattttaca acaaaagagc ctgatgatga gcaattagag gttgctttgg
    37801 aagcattgaa agcagtagta gattagagaa aaggatattt gttatgacga taaaagatat
    37861 aattattaaa tattcagata aactaaaaga tataagtgat actccaagat tagatacaga
    37921 gttattgtta caaaaaacat tgggtgttga tagattatat attcacttaa atctgaataa
    37981 agagctaact gaagaacaaa aaacaaaatt tatgggtttt gcagaagaaa gattaaatgg
    38041 aagaccaata gcatatatag ttgaaaatag ggagtttatg ggattagatt tttttgttaa
    38101 ggaaggcgta ttgattccaa gaccagatac agaaacttta gtagaagaaa taattgaaat
    38161 atgtagagaa aaaaaagatg taagtatatt agatatagga actggttctg gagctattac
    38221 aataagttta gcaaagtaca ttgaaaattc taaaatcatg tcttttgata tatcagagac
    38281 agcattagaa atagctaaaa aaaatgctat tataaatgaa gttggtgaaa aaataaagta
    38341 tattaattct gatttattta cagcaataag tgatagtaac ataaaattcg atatcatagt
    38401 ttcaaatcca ccatatatta aaaaacaaga tatagaaact ctgcacaaac aagttaaaga
    38461 ttatgaacca tataatgctc ttgaaggtgg agaagatgga ttagactttt atagaagaat
    38521 aacagaacaa ggtaagaaat atttaaataa gtgtggaata ttagcatatg aagttggtca
    38581 taatcaagct gaagatgtaa taaatattat gaaaagtaat ggatataaaa aaatatacac
    38641 aaagaaggat attcaaggca ttgatagagt tgttataggt tacaatatat gatataatca
    38701 acaattgata tgttacattt aatcgaggtg aaagtatgtt aaaaaaatta gaggtattgg
    38761 aagatacata taaagactta agtgaaaaaa taggtgatcc ggatgtaata aatgatcaaa
    38821 aagtttggca gaaatatata aaagaacatg ctgacttgga acctattgtt atgaaataca
    38881 gagagtataa aagcgtttta gatagtataa aagaatcaaa agaaatttta caagaagagt
    38941 cagatgaaga attaagagaa ttagctaaaa tggagttagc tgagatggaa gaaaaagtag
    39001 cacctctgga ggaagaaata aaaatactat tattgcctaa agaccctaat gatgataaga
    39061 acgttatagt tgaaattaga ggtggagctg gaggagatga agcagctcta tttgctggtg
    39121 atttatttag aatgtactca aggtatgctg aaagaagaag atggaaaata gaattattga
    39181 gtgcaagtga tactggagtt ggtggatata aagaagtatc ttttatgata aaaggtaaag
    39241 gtgcttattc aagattaaag tatgaatctg gagttcatag agttcaaaga ataccatcaa
    39301 ctgaatctgg tggaagaata cacacatcaa cttctactgt tgcagtatta ccagaagttg
    39361 aagatgtaga ggttgaaata aatccaaatg atttaagaat agatgttttc cgttcttcag
    39421 gtaatggagg acaaagtgtc aatactactg actctgctgt tagggtaaca catataccta
    39481 caggtgaagt agtatcttgt caagatggaa aatcacaatt aaaaaataaa gagcaagctt
    39541 taaaaatatt aaaagctaga ctttacgata aagcattggc agaacaacac aaagatatag
    39601 cagcagaaag aaaaagccaa gtcggaacag gagacaggtc agaaagaata agaacatata
    39661 atttccctca aggtagaata agtgaccata gaataaattt aactttatat aagttagatg
    39721 cattcttaga tggagatata gatgaaatga tagatgcttt aattactgtt gaccaaacag
    39781 aaaaaatgac tgcaatataa gtaatgtttt aaagaagctg tttcttccta gaaattattt
    39841 tctattttga aacagctatt ttgcatttta ggtcatgtta tcatattcta ttgtatataa
    39901 ttgttatata caagtatagg agggttatat gatactaaaa gtaacaataa ttgggctttt
    39961 ggcaggtgtt atagggacag gattaggagg agtaatatca gccattttta aaagagaggt
    40021 cgataaatat ctaagttttt ttatgggatt atctggtgga ataatgttgg ctgtagtggt
    40081 gtttgattta atgaaagaat ccatggacaa aatggggata atcaatacag ttatatttac
    40141 gtttgttgga gctctcataa ctatgtacat aaaaacaaaa ttagatgtgt ctggaaatat
    40201 ggcatctgga tatcttatat tcataagtat attactacat aatttaccag aaggactagc
    40261 cattggttct tcttttatgt caactgaaag tttaggtatt acacttgcta tagttatagg
    40321 tctacataat ataccagaag ggttagctat ggcattaggc ttagtttgca ataaaatgaa
    40381 actaagtaaa gttattttgt ttactgtaat agctggatta cctatgggtc taggaagttt
    40441 tttaggtgtt tattttggag gagtatttac ttctcttata ggagtatttc ttgctacagc
    40501 aggaggtact atgatgtatg ttgtattgga agaaattttt ccacattcaa aaagtgtgta
    40561 ttgtataatt ggtttcttgt taggtacaat gattgtaaat tatatataaa attaaaatga
    40621 aagcaggcat acaaaagatg aaaactatta taagtaatat tgatataaat aatattgatt
    40681 atgaggaaat aaaaatacaa gcaaaacttc ttagagaagg taaaacagtt atttttccaa
    40741 ctgagacagt atatgggttg ggtgcaaatg cattagatga aaatgcagta aagaagattt
    40801 atgaagctaa aggaagacct agtgacaatc cattgatagt ccatatatat gaaaaagaag
    40861 aagtatatga cttagccaaa gatataagtg ataaagctaa acttgttata gaaaagcttt
    40921 ggccaggacc tataacaatt atattaaata aaaaagatat aattccatac aaaactagtg
    40981 gagggcttga tacagttgca ataaggatgc cttctaatgt tattgccagg gcaataataa
    41041 aagaagctgg tatacctatt gcagctcctt ctgcaaatat ctcaggtcgc ccatctccaa
    41101 caaaagcaaa acatgtatat gaagagatga atggaagagt ggatggaata gttttaggag
    41161 gagacagtaa ttttggattg gagtcaactg tattggattt aacagaagag actcctatga
    41221 tactgagacc aggaagtata actaaagaag ttttagagga acttcttgga gaagtaaagc
    41281 ttgacccatc attaagtaaa aaagaagata atcaaaaagc taaagctcct ggaatgaaat
    41341 ataaacatta ttctccaaat gcagatgtat atataatatc tggaaaaaga gaaaatgtag
    41401 caacaaagat taatgatatg ataaaatcta atagagacaa aggtctaaag acaggtgtaa
    41461 tgtgtattag tagaaaccga gagttttatg atggagaagt aatcgattta ggaaactctc
    41521 ttgaagaagt tgcaagtaat ctgtttgatg ctcttataga aatggacaaa aagggtgttg
    41581 atgtcatata ttcagaagag ttccctaaga ctggagttgg acaagcaatt atgaatagac
    41641 tactgaagtc agcaggatac aaagtgataa aggcttaaga gggagttgta aaatatgaat
    41701 atacttattg tgtgtacagg taatacttgt agaagtccta tggctgaggc tatactaagg
    41761 aaagcaatta aagaatctgg aagaagtatt gaagaatatt ctatatcatc agcaggtata
    41821 tctacagcaa atggaatggg tgcttctgaa aattctatag aagtactaaa agaaatcggt
    41881 atagacttat caaatcatag aagtaaagta attactaaaa aattaattga tgaatcagat
    41941 ataatactaa caatgactaa atctcataaa gaaattttag tacaagcagt tccaaaatgc
    42001 aaagaaaaag tgtatacttt taaagggttt gcaaataaaa atgaggaaga tatatctgac
    42061 ccatttggtg gaaatttgga tatttataga agcactatga gagaaataat gtattctgtc
    42121 aacgaaatag taaaaaaaat atgaaacgga ggataaacaa atgaagatag gattaggctg
    42181 tgaccatgga ggatataatt taaaaaaaga aataataagc tatttagaaa gtaaaggcat
    42241 agaatgtgta gattatggaa ctaataatgc tacagattct gttgattatc ctgtttatgg
    42301 agaaatagtt gcaaattcag ttataaataa agaagtagat tatggaatat tatgttgtgg
    42361 tacagggata ggaatatctt tagctgctaa taaggttcct gggataagat gtgcagttgt
    42421 atcagatgta ttttctgcaa aaatgtcaaa agcacataat gatgcaaata tgttatcatt
    42481 aggcgaaaga gttttaggaa aaggtctagc acttgagata gttgaagcat ggattaatac
    42541 ggattttgaa ggtgatagac atgctagaag agtaaacatg attaaatcaa tagaagaaaa
    42601 acataataaa taataaaaat tacagtttta tcaggaggaa ttatcaaaat gagcaaagtg
    42661 gtagagacaa accatccatt aatacaacat aaattaactt taatgagaga taaaaatact
    42721 ggttctaaag actttagaga gcttttaact gaaattgcaa tgttaatggg gtatgaaata
    42781 acaaaagata taccattaaa agatgtagaa atagaaactc ctattcaaaa aacatcatct
    42841 aaagtagttg caggtaaaaa attagcaata attccaattt taagagcagg tcttggaatg
    42901 gtagatggat tagtaagctt aatgccagca gcaaaagttg gacatgtagg actatataga
    42961 gacccagaaa cattaaaacc agttgaatat tattgcaagc ttcctcaaga cataggagaa
    43021 agagatataa tagtagttga ccctatgctt gcaacagggg gttctgctgt agcagctata
    43081 gatttactta agtctaaagg agctaaaagt ataaaattag ctaatttagt agctgcacca
    43141 gaaggtatag cagaagtgca aaaatatcat gatgatgttg atatatatgt tgcatcagtt
    43201 gacgaaagat taaatgaaca tggatatata attcctggtc ttggtgatgc tggagataga
    43261 ttgtttggaa ctaaataaaa ataaataact ataaatattt attagggact ttcttatata
    43321 gtatatataa ttttatgtaa gaaggttctt ttttatatat tttttctcaa aataggaatt
    43381 attgaattga taaatttctc gaattgagaa taaaaagttt ttaccaatta aaaaagattg
    43441 cttattttac aaggttttat agaatagtag tctattttaa tgtggcatgt tagttgctta
    43501 cgattaagat tataggtatt aagtgtatta gttaaattga gaattgaata ttaataatat
    43561 tattgggagt ggtaatacat gaagaattgt atagtagtaa gaggggctgg agatttagca
    43621 acagcagtta ttcataaatt acacaacagt ggttttaaag tagttgcgtt agaaatagaa
    43681 gaaccattag ctataagaag gcaaatttct ttttgtgagg ctgtttatga aaaaaaagta
    43741 actgttgaag gtgtagaatg tatattttgc aatactgaaa aagaaataga agaagtttta
    43801 tctcaacata aagtagcctt gattatagac ccaaaaggag aaaaattatc taaattaaat
    43861 ccaatcgttt tggttgatgg aatactcgct aagaaaaact taggtacaac taaagatatg
    43921 gcaccactta caatagcttt aggtcctgga ttttgtgcag gagaagatgt agatgttgtt
    43981 atagagacaa tgagaggtca taatctagga agaataataa ctaatggtag agcagaaaaa
    44041 aatacaggag taccaggagc aataaaaggt ttttctaaag agagagttat gcattcacag
    44101 tatgatggca taatggaaaa tgtatgtaag ataggtgata tagttgaaaa gggagaccta
    44161 ctttgctata ttaataatta tgaaaaaaaa catgaagtta gagcaacaat atctggagtc
    44221 cttagaggtt tattaaagga tggagcaaag gtaaacaaaa atttaaaaat actagacata
    44281 gaccctagaa aagaagaagt taagaatgct tttacaatat cagataaagc tagatgtata
    44341 gctggagggg ttttagaagc catactaaac aatgggattt atccccaaaa ttaatttata
    44401 ttagataaag ctattaatat tttattaaga tgatataata aatttaatag attaaagaga
    44461 gcatatgacg ggtatttaaa atttaaatgg taaaaatgct ctttttttat tggacattca
    44521 attaacatgt gtatataata taaaaataag tatttattta ggttgaaatc taataaatat
    44581 ataataccag tacataagca aagaggagaa aagtaaatta tgagaccatc atgggatgaa
    44641 tacttcatgg aaattgcaga agtagtgaaa aagcgttcaa cttgtattag aagacaagta
    44701 ggagctgtaa ttgtaagaga caaacaaata ttaacaactg gatataatgg ttctccgaga
    44761 aatcttgaac actgtgaaaa tattggatgt aagaggcagg aattaaatat tccctcagga
    44821 gaaaggcatg aattatgcag agcattacat gctgaacaaa atgcgataat acaagctgct
    44881 cataatggta ttagtgtaga tggggcaact ttgtatgtta ctacaagacc ttgtgttttg
    44941 tgtgcaaaaa tgtgcataaa tgcaggtata ttgaaaattg tttatgaagg tgattatcca
    45001 gatgatatgt ctacagaatt attaaaagaa gcaggaatag aactcataaa attttagtgg
    45061 ataaaattta taagacaata aatgtcaaat tatgacactg aaatacaatg gtatcaaatg
    45121 attaaaagaa agtatttgaa aaatcatcag attagtagta agatatattt tataattaaa
    45181 taaatttatg agatagatta attacatgtt atttatttta taataatgag ggatactatt
    45241 tatgagtaaa agaaatacac atacagaaat tgcaaagttg tttagtttaa taagtcaaat
    45301 aggattgatg attgtaatat caatattagg atgtactttc ttaggaaaat ttttagatag
    45361 taaattgaat actactccag ttctgacaat aatattttta ttgttgggtg ttggaggtgc
    45421 ttttagtggt gtttataaaa ctctaatagt atatacacga aggaagtgat atattggata
    45481 taaaattact taaccaggta aggtcagtaa caaaaggtat agtaatattt gatgtagttt
    45541 taattgtgct aatgttgata acatcaacat taagtaaacc agtattatta ggacttatat
    45601 ttggctctat aatagctatg ttaaatttta gattattggc gatatcactg gaaaaatcag
    45661 tgacatttcc tgctgggaag gcacaagctt atgcatcagc acaatatgct gttagattat
    45721 ttataatggc agttgttcta attgcatcag ctacagtacc acatttaaat ataatcggaa
    45781 cagcattggg attattgagc acaaagtttg tcatattatc aaaaaacttt ataaataaat
    45841 taaagaggaa ggaggcataa gtatgaatca agcgcattat tatctcatag gaaactttaa
    45901 gattagtgaa acaatactag taagttgggg aataatggcc tttttagcga tagcatctta
    45961 ttttttgact agaaacttaa aaaaagttcc aactagtaaa gttcaaatat tcttagaatt
    46021 tgcagtaggt ggtcttgcta aaatggttac agatgttatg ggtaaaaata cagtaaaaag
    46081 aatgccatat atgataccat acataggaag cttgttttta tttttcgcat gttcaaattt
    46141 aagcccatta cttggattaa ggtcaccaac aaccgactta gacacaactg ctgcatgggc
    46201 aatgattaca ttttttatga tttattttgc aggagttaaa tttaaggggt tatcatactt
    46261 taaagaattg gctcaaccac ttgccataat gttaccaatc aatattatag gtgagttagc
    46321 tagaccaata tcattaagtt tccgtccatt tgggaatatt ttaggtggaa caataataat
    46381 gggtctgttt tatcaacttt taggttttat atctagttta ataccaggat tatcaatacc
    46441 aattggagaa atattaatac cagtcccatt acacttgtac tttgacttat ttgcaggatt
    46501 gttacaagca tatatattta taatgttaac aatggtattt atatcaagtg caggagagtt
    46561 agattaagaa atcaacttat attaagataa aaataaaata atttaaaaaa ttaaagtttt
    46621 aaggaggaaa taattatgga aacagcaata gtagcggcag catcagcaat aggagcaggg
    46681 atagcagttg caacaggtat aggtgcaggt ataggtcaag gtatagcagc agctaaagct
    46741 gcagaagcag taggaaacca gccagaagct aaaggtgata taacatcaac tttattacta
    46801 ggagttgcta tagcagagtc ttcagcaata tatggtttgg ttatatcaat aatacttcta
    46861 ttcgtaaatc cattctttaa atacttagga atgtagattt agtccttaaa tattttgaga
    46921 taggtttata ggtaaatcat ctcaaactta taatcatgag atgaacttta tcatctcgtg
    46981 attataagta gttatgattt atggaaagga ggataattat ggaaaaagca ctagtaggta
    47041 taacgtggga atttgtgttc caaattgtta atacttttat aatattttta cttctgagaa
    47101 agcttttatt caagccagta ttaaatataa ttgaatcaag agaaaatgat attaaaagtg
    47161 atttggcaga aggcgaaaaa gcaaaaaatg aaggtttagc tctgaaaaaa gaatatgagt
    47221 caaaaatcaa ttttgccaaa gatgaaggtc aagaaatcat aaaacaagca actataagag
    47281 cagagcagaa atcagatgat atagtaaata ctgcaaagaa agatgctctt gatataaaag
    47341 aaaaagcaaa caaagatata gaacaagaaa gacaaaaggt tataaatgaa atcaaaaatg
    47401 atatatctaa catagcacta cttgcagcat ctaaagtcat tgaaaaagat ttagataaat
    47461 ctaaacatga agaattgata gaaaacttta taaaagaggt aggggaagct aaatgataaa
    47521 tgtaatagca aatagatatg ccgaagccct atttcaatta ggagaagaag aaaattcaac
    47581 tgatgtctta ttcaaagaac tagaaaaagt agtagatatg atgactaaag ttagtaaaga
    47641 tttctacaaa gttttaaaat caccattagt ttcaaaatca gaaaagaaaa acttggtaga
    47701 aattattttt agtaaagaag taagttctaa cataaaaaac tttttaaaag ttttagtaga
    47761 taaagataga atatcatatt tagaagatat cgaattagct tataaagaat tattaaataa
    47821 gaaaaacaat gtaattgatg gagtagcaat aagtgctatt ccaatgagtg aaacagatat
    47881 taaagaactt gaagtcaaac tttcaaataa atacaacaaa aatgttacta tagaaaatgt
    47941 tgttgacaag actatactag ggggagttct agttagaatt ggaaatgaac aaatagatgg
    48001 cactgtgaaa actcgtcttg ataagatgaa agaaaagcta tctgaagtaa tatcttaaga
    48061 atggcggtga acccatgaac ttaaaacctg aagaaataag ttctataatt aaacagcaaa
    48121 taaaaaatta tgagaataaa gttgagttga cagatacagg tagtgtttta acagttgggg
    48181 atggtatagc gagtgtatat ggattagaaa aagctatgtc tggtgagttg ctagagttcc
    48241 caggtgaaat atatggtatg gcacttaact tggaagaaga agtagttgga gctgttatcc
    48301 ttggagatga cagtgaaata aaagaaggcg atatagttaa gagaactgga agaatagttg
    48361 aagttccagt tggagaagct ttaataggta gagttgtaaa ctcactaggt cagtcaatag
    48421 atggaaaagg acctatagcg tacacaaaaa ctagaccagt tgaatctgag gcacctggta
    48481 taatagatag aaggtctgtt tatgaaccat tacaaacagg aatcaaatca atagattcaa
    48541 tgataccaat tggtagaggt caaagagagt taataatagg tgacagacaa actggtaaaa
    48601 catctatagt tattgacact atactaaatc aaaaaggaaa agatgttatc tgtatatatg
    48661 tagcaattgg acaaaaacgt tctactatag ctcaattagt tagctcttta gaaaaaggtg
    48721 gagctttgga ttatacaata gtagtttcag caacagcatc tgaatcagca ccacttcaat
    48781 atatagcacc atatgctgga gcagctatgg gtgaagaatt tatgtacaat gggaaacatg
    48841 tacttatagt gtatgatgat ttaagtaaac aagctgttgc atatagagaa atgtcattgt
    48901 tacttagaag accaccagga cgtgaggcat atcctggaga tgtattctat ttacattcaa
    48961 gattacttga aagagcagct aagttatctg atgaattggg tggaggttca atgactgcct
    49021 tgcctataat agaaactcaa gctggtgacg tttctgcata tataccaaca aatgttatat
    49081 ctataacaga tggtcaaata tacttacaac cagagttatt ctattcaggt gtaagaccag
    49141 cagttgaccc tggtatatca gtatcaaggg ttggtggttc tgcgcaaatt aaagcaatga
    49201 aaaaagttgc aggaacatta aaacttgcat attcacaata tagagaactt gcagcattct
    49261 cacagtttgg ttctgactta gatgaagata ctaagaagag acttgctcaa ggtgaaagaa
    49321 tcgttgagat attaaaacaa ggtgagcatc aaccaataaa agttgaaaat caagttatga
    49381 taatatatgc tgttataaat aatcatttag aggatattcc gatagataat atagctagat
    49441 ttgaatcaga attgtatgca tttgtagata ataattatcc ggaaatatca agaaaaatat
    49501 taggtggaga agacttcact catgatttaa cagatgcaat aaatgaattt aaagaaaagt
    49561 ttgttgttga agtataatcc tttttaaaaa aggaggtaaa ttaattggca ggagctggaa
    49621 tcaaagcaat caaaacacgt atgggaactg ttgaaagtac aaagcaaata actaaagcaa
    49681 tggaattagt tgcatcttct aagcttagaa aagcaaaaga aaaggctgaa gattcaagag
    49741 aatattttaa tactttatac aatacagtaa aagatattgc ccaaacaaca cgtggaatta
    49801 gaaatgaatt tttaaaagaa agagaagtaa aaaataaatg ctatgtagta gtagctggag
    49861 acagaggact tacaggtgga tacaattcta atgtattaaa ggaatctgtc tctcatatga
    49921 aaggtaaaaa ggaaacagtg attactgttg gaaagaaagc acatgaattt ttttctaaaa
    49981 gaaactacaa tatagcaaaa gatataaata gtgttgaagc ttgtggatat gaagatgcga
    50041 cagaaatttc acaaactatt attgacctgt ataaaaaagg tgaagtagat gaagtttata
    50101 ttgcttatac aaaatttaaa tctgctttat cacaagaagt tgaaattttg aaattgcttc
    50161 cattagtctt tgaaaaagat acagacgaac aaggaatggc agtaaaagaa gaaaaacaaa
    50221 atagatcaaa agtacaatat ataccatcag cagaagcagt tttaagttat attgtacctc
    50281 agtttatatc aggtacagta tatggtggaa ttttggaatc ttttgcatca gagcaaggtg
    50341 ctagaagaac tgcaatggaa tcagccacag acaatgcgaa tgaaatgcta tctaatttag
    50401 agttaagtta taacagagca agacagtcag ctgtaactca agaaataaca gagatagttg
    50461 gtggagttga agctctaaaa tagggaggtt aggaaatggc aaatgtaggt aaagtagtac
    50521 agattgtagg agctgtacta gatgttaagt ttgatagtga acaaagccta cctaacttat
    50581 taaatgcgtt agtaataaag ttaggagata aagaaatagt tgcagaagtt gctcaacata
    50641 taggtgatga tactgtaaga tgtatagcta tgagtgcaac agatggactt gtaagaggta
    50701 tggaagttgt agatacagga gggccaataa gtgttcctgt tggagatgaa actttaggta
    50761 gaatattcaa tgttcttgga aaaccagttg atgggaaacc agctccaaaa tctgcaccta
    50821 aattacctat acatagacca gcaccggcgt atgatgaact agaaactaca gcagagatac
    50881 tagaaactgg tattaaggta gttgacttac tagctccata tctaaaaggt ggaaaaatag
    50941 gtctatttgg aggagcagga gtaggaaaga cagttctaat acaagagctt ataaacaata
    51001 ttgctaagca acatggtggt atttctgtat tttcaggagt aggagaaaga acaagagaag
    51061 gtaacgacct ttatggcgaa atgagtgagt ctggagttat aaataaaaca gctctagtat
    51121 ttggtcaaat gaatgaacca cctggagcga gaatgagagt tgctttaact ggacttacaa
    51181 tggcagaaca ttttagagat gagcaaggac aagacgtttt acttttcgtt gataatatat
    51241 tccgtttcac acaagctggt tcagaagttt cagcacttct aggacgtatg ccatcagctg
    51301 ttggttatca gccaacatta gctactgaaa tgggtgcact tcaagagaga ataacatcaa
    51361 ctaagaaagg ttcaataaca tctgttcaag cagtatatgt accagcagat gacttaactg
    51421 acccagcacc agctacaaca ttctctcact tagatgcaaa aacagtttta tcaagacaaa
    51481 tatcatcact gggtatatat ccagcagttg accctcttga atctacatca aggattcttg
    51541 accctagtat agtaggaaaa gaacattatg aagtagctag aggtgtacaa tcaatacttc
    51601 aaagatataa agaacttcaa gatataattg ctatacttgg tatggatgaa ttatctgatg
    51661 aagataagtt aatagttgca cgtgctagaa agatacaaag attcttatct caatcattca
    51721 cagttgctga gcaatttaca ggaaatccag gtcaatatgt tcctgttaaa gagactgtaa
    51781 gaggatttaa agaaattctt gaagggaaac atgatgactt accagagtca gcattcttat
    51841 ttgtaggaac aatagaagac gctgttagaa aagcgaaggg aagtatgtag tcatggccag
    51901 tgaattttca ttgaaagttg taactccaag taatactttt tatgatggag atgttgaaat
    51961 gatagtagtt agaactacaa aaggagacaa aggggtgttg aaaggtcata taccatttgt
    52021 ttcaggtata cctgtaggaa ctttaaagat aaaaaaagag ggaagtttta aagaggcgaa
    52081 aatagcagaa ggattcatta atgtaactca agataaaact gtcatattga cagaatctgc
    52141 tgagtggatt taataaatta atttagagta taaaaagtca ttttgaccaa aaaaactttt
    52201 gggtaaaatg gcttttttgc gttttggtgt taataaaata acgataaaat tgtaagtttt
    52261 tagtgataat aaattcacaa aatgtttaaa tttactgcca aattatgttg taattaataa
    52321 gataaagaag atttagatag cttttatgta agtgaaaaag tcttcatata agaaatacat
    52381 ataaaatttt tataaaattt aaaaaatttg cttagttgtg tagtataata aataatataa
    52441 tagatttgta tataatattc attacaaata attttaaaag ttacagtttt attataatga
    52501 ttattaaatt aagataagta acataaacta aataataatg tgtattatcg atagtaaatt
    52561 tataaattca ttattaatta ttgaaagaag tattataaat tttctggagg aacaatgaat
    52621 atatttgata ttggcgtaga tataattgaa attgatagaa taagaaaggc tgtagacaaa
    52681 aataataggt ttttggagaa gatatttaca gatagagaaa tagagtattt taattctaag
    52741 aattttaaag cagaaagtat agcaggtaat ttcgcagcca aggaagctat aagtaaatct
    52801 ataggtacag gaattagatt gtttaatttt aaagatatag aagttctcag aaatgaaatg
    52861 ggaaaaccaa tagtaaaaac ttataataat ctagcaaaaa tgtgcataga ttataatgtt
    52921 ttagaaataa aagtttcaat atcacattca aaagattatg ctatagcaaa tgcgataacc
    52981 ataattaagg actaaggagt gattacatta tgatggataa aacagcaaaa gaaataatga
    53041 caacagatgt aatagtagca aaacaagatg atagtatagc agatgtagct aatatgctaa
    53101 ttgcagaaaa aataggaggt ctgccagttg tagattcaga gaatagagtt gttggaataa
    53161 tttctgaaac agatattctt aaaaaagaaa aatatataga agcacctctc tatataaatc
    53221 tattacaagg attgattttc ttagatgact taaaaaaagt tgaaaaagat ataaaacaag
    53281 tagctgctta taaggttgga gaacttatgt ctaaagatat aataaaggtt catgaagatg
    53341 ataagtttga tgatgtagct aatgtaatga ttaaaaaatc aataaacaga gttcctgtag
    53401 tagatgatga caacaaactt aaaggtataa tttgtagata tgatataata aaagctttat
    53461 acaatgaata aactgaagta ggaggtgtga tgtgagaaaa aagtggacca tagtatgtat
    53521 tatgttcttg gcactggtta taattgtaat aggatgccaa aaacgacagt ccacaaaaga
    53581 agaagtatac aaagattttc aaaagcagat ttctgatatg aattattaca gttgtaaagc
    53641 tgaagtagaa gtagttggaa ataaaagtcc acacaattat gttttaattc atacttataa
    53701 aaaaaccgat aactataaat tagaagtaat ttccccaaaa catttaaaag ggaaaagtat
    53761 agaatatcaa ggtgataaaa tattagttaa aaatccaaag attagtgatg tagtggagtt
    53821 acctaataca ggaaaaaata atcaatatct ttttgtagga gactttataa aaaactatct
    53881 tcaaaatgag gagatgaaag taaagttatc caaaggacat ttggttttag agacttttat
    53941 tcctggggat aataaatatt ttaacaagca agtattatat gtaaatgctg acacaaaaaa
    54001 tcctgaaaaa atggaagtgt tagataaaga gggagtgcca agatttacag taaaatacaa
    54061 agattttgaa tacagaaact aagggggact aaagacatgc aaaaaataac agtgcctaca
    54121 tgggcagaga taaatctaga taacttaaga tttaacttaa ataatattaa aaatttatta
    54181 gaagaagata ttaagatttg tggagtaata aaagctgatg catatggaca tggtgcagta
    54241 gaagttgcaa aattgctaga aaaagaaaaa gtagattact tagcagtagc aagaactgct
    54301 gaaggaattg aacttagaca aaatggcata acacttccta ttttgaactt gggatatact
    54361 ccagacgaag cttttgaaga ttctataaaa aataaaataa ctatgacagt ttattcttta
    54421 gaaacagcac aaaagataaa tgaaattgca aaatctttag gagaaaaagc ctgtgttcat
    54481 gttaaaatag actcagggat gactagaata ggtttccaac ctaatgagga gtcagtacag
    54541 gaaataatag aattaaataa attagaatat atagatttag aaggtatgtt tactcatttt
    54601 gctacagctg atgaagtaag taaagagtac acttataaac aagctaataa ttataaattt
    54661 atgtctgata aattagatga ggctggtgta aaaatagcta taaaacatgt atcaaacagt
    54721 gcagctatta tggattgccc tgatttaaga ttaaatatgg taagagcagg aataatatta
    54781 tatggtcatt atccatctga tgatgtattt aaagatagat tagaattaag accagccatg
    54841 aaattaaaat caaaaatcgg acatataaaa caagttgaac caggtgtagg aataagttat
    54901 ggactaaaat acacaactac aggtaaagaa acaatagcta cagttccaat aggatacgca
    54961 gatggattta ctagaatcca aaaaaatcca aaggttctta ttaagggaga agtgtttaat
    55021 gtagttggta gaatatgtat ggatcaaata atggttagaa ttgacaaaga tatagacata
    55081 aaagttggag atgaggttat actatttgga gaaggcgaag ttacagctga gcgtatagct
    55141 aaagacttag gaactataaa ctatgaagtg ttatgtatga tatcaagaag agttgaccgt
    55201 gtttatatgg aaaataatga gcttgtacaa ataaacagtt atttgctaaa ataaaaccat
    55261 gatattataa tctgggaggc gattttgtgt acgataagag taaagaaaaa atagaagtta
    55321 ctttacctga ctctctgatt tctgaagttg atagtatagt tcagatggag aacagtaaca
    55381 gagaagattt tgcaaaagct gcttttcagt tctatataac tcagaaaaag aaaattcata
    55441 ttaaagaatc tatgaaaacg ggttatagag aaatgggtca aattaatctg tcactagctg
    55501 aattaggtat gacagttgaa gaagtatctt ctgatgataa aaatgaaggg aagattgccc
    55561 aaggtgagta ctaattgtga aataaagcgt ggtgatttat attacgctga tttaagtcca
    55621 gttgttggtt cagagcaggg aggcgttaga cctgttctga taatacaaaa tgatgtaggt
    55681 aataaatata gtcctacagt tatagtatca gctataacat ctcagattaa taaagccaaa
    55741 ttacctactc atgtagagat aagttccaat gaatatgggc ttaataaaga ctcagtaatt
    55801 cttttagagc aaattagaac tatcgataaa aagagactga gggaaaaaat tggttgtcta
    55861 gatgaaaata tgatgataaa agtagatgat ggacttcaaa taagtttagg attgtttact
    55921 ttttaaagag ggatttgtct tttattagac aatccctttt ttcttttaaa tatctcttaa
    55981 ggtttaattt taatgtacta tgttaataaa attaaaaata acgcaaaaaa atagcataaa
    56041 agtgatataa taatatggaa ttgatataaa attacgaact caagggaggt aattgcatga
    56101 gagaccataa aggattaaat gagattgcgc gtatcataag aagagatata gtttcaatga
    56161 tacatagagc caaatctgga catccaggag ggtcattatc agtagttgaa atacttactg
    56221 cgttatactt tgatgaaatg aatgtggatt cttcaaatcc taaaatggag gatagagata
    56281 gatttgtatt atcaaaagga catgctgcac cagcattata tgctacattg gctgaaaaag
    56341 gttattttga ttaaaaaata gctacgcaca cttgtgactt tagtcatgag ttagtagcta
    56401 aaatagtcag catataggga aacctatatg tagagatagg atagaaccta tccaatacta
    56461 cttgaattgc tggaaacccc taaagctaac caaactaaaa cataaggatg aaataaacct
    56521 aagtgtgaaa gttacgaaag tagaaaaaat tggttagatg gtataaggtt aaaccctaag
    56581 tactttaaaa tgggcaatca gcaggtaagc tccgaatagg agaaacttca acgactattc
    56641 ctcttgaggg aagtacacta caagttatcg gtagtggaag tgggtagacc ttaacggata
    56701 atgccgaagg ataagatata gtctgtgctt gcatgaaagt gtaagaagtt cataagagaa
    56761 ctgcataggt agtagcgaac ttatgtgaac gacaacctca aaaatgataa aagttaagac
    56821 ttacggttct taaatatgtt a
//
LOCUS       DAEMHW010000003        53111 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEMHW010000003 DAEMHW010000000
VERSION     DAEMHW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.003"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..743
                     /locus_tag="KPK67_000119"
     CDS             <1..743
                     /locus_tag="KPK67_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2457144.1"
                     /translation="YEEIGILRPVRDINGYRMYNISDIWKLNLIKEFRSLNFPMKKIK
                     EYLDDRSIESTKNILNEELDLIDKKIAEFISHKENIIKRLSSIESVIQNTKIDEIEVV
                     YIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRFNILGNNNIGSAFSTEAINKGIFN
                     EFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNSNNKMYMEKVFKFIEENNYKIIAD
                     PIEIYKIDIHETGIVEEFVTEIQVPITK"
     gene            888..1127
                     /locus_tag="KPK67_000120"
     CDS             888..1127
                     /locus_tag="KPK67_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457145.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1315..1491)
                     /locus_tag="KPK67_000121"
     CDS             complement(1315..1491)
                     /locus_tag="KPK67_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457146.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1545..1641)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1996..2670)
                     /locus_tag="KPK67_000122"
     CDS             complement(1996..2670)
                     /locus_tag="KPK67_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG2457147.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVISGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     gene            complement(2890..3444)
                     /locus_tag="KPK67_000123"
     CDS             complement(2890..3444)
                     /locus_tag="KPK67_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG2457148.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            complement(3522..3887)
                     /locus_tag="KPK67_000124"
     CDS             complement(3522..3887)
                     /locus_tag="KPK67_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2457149.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            4352..5137
                     /locus_tag="KPK67_000125"
     CDS             4352..5137
                     /locus_tag="KPK67_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2457150.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISE
                     YKDFYKKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(5234..6058)
                     /gene="pdaA"
                     /locus_tag="KPK67_000126"
     CDS             complement(5234..6058)
                     /gene="pdaA"
                     /locus_tag="KPK67_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG2457151.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            6279..9398
                     /locus_tag="KPK67_000127"
     CDS             6279..9398
                     /locus_tag="KPK67_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG2457152.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     NFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            9416..10015
                     /locus_tag="KPK67_000128"
     CDS             9416..10015
                     /locus_tag="KPK67_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBG2457153.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(10242..12371)
                     /locus_tag="KPK67_000129"
     CDS             complement(10242..12371)
                     /locus_tag="KPK67_000129"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBG2457154.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCHHEKKHI
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYDKKDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLARPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            12637..12885
                     /locus_tag="KPK67_000130"
     CDS             12637..12885
                     /locus_tag="KPK67_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457155.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHYRDIKRKIS"
     gene            13051..14442
                     /locus_tag="KPK67_000131"
     CDS             13051..14442
                     /locus_tag="KPK67_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBG2457156.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            14557..16026
                     /locus_tag="KPK67_000132"
     CDS             14557..16026
                     /locus_tag="KPK67_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG2457157.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            16091..16684
                     /locus_tag="KPK67_000133"
     CDS             16091..16684
                     /locus_tag="KPK67_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBG2457158.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            16866..18212
                     /locus_tag="KPK67_000134"
     CDS             16866..18212
                     /locus_tag="KPK67_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2457159.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     SLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            18394..18765
                     /locus_tag="KPK67_000135"
     CDS             18394..18765
                     /locus_tag="KPK67_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBG2457160.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            18758..19447
                     /locus_tag="KPK67_000136"
     CDS             18758..19447
                     /locus_tag="KPK67_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBG2457161.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            19967..20833
                     /locus_tag="KPK67_000137"
     CDS             19967..20833
                     /locus_tag="KPK67_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2457162.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            21075..21884
                     /locus_tag="KPK67_000138"
     CDS             21075..21884
                     /locus_tag="KPK67_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457163.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            22028..23092
                     /locus_tag="KPK67_000139"
     CDS             22028..23092
                     /locus_tag="KPK67_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG2457164.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            23120..23839
                     /locus_tag="KPK67_000140"
     CDS             23120..23839
                     /locus_tag="KPK67_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2457165.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            23863..24447
                     /locus_tag="KPK67_000141"
     CDS             23863..24447
                     /locus_tag="KPK67_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBG2457166.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEKLNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            24449..25798
                     /gene="pabB"
                     /locus_tag="KPK67_000142"
     CDS             24449..25798
                     /gene="pabB"
                     /locus_tag="KPK67_000142"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBG2457167.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            25788..26531
                     /locus_tag="KPK67_000143"
     CDS             25788..26531
                     /locus_tag="KPK67_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBG2457168.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            26565..27266
                     /locus_tag="KPK67_000144"
     CDS             26565..27266
                     /locus_tag="KPK67_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457169.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDINYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISYIIKDISRDIYLEGPSKISLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIL"
     gene            27291..27863
                     /gene="folE"
                     /locus_tag="KPK67_000145"
     CDS             27291..27863
                     /gene="folE"
                     /locus_tag="KPK67_000145"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBG2457170.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            27927..28718
                     /gene="folP"
                     /locus_tag="KPK67_000146"
     CDS             27927..28718
                     /gene="folP"
                     /locus_tag="KPK67_000146"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG2457171.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            28729..29094
                     /gene="folB"
                     /locus_tag="KPK67_000147"
     CDS             28729..29094
                     /gene="folB"
                     /locus_tag="KPK67_000147"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBG2457172.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRARDE"
     gene            29087..29593
                     /gene="folK"
                     /locus_tag="KPK67_000148"
     CDS             29087..29593
                     /gene="folK"
                     /locus_tag="KPK67_000148"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBG2457173.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            29574..30359
                     /gene="aroF"
                     /locus_tag="KPK67_000149"
     CDS             29574..30359
                     /gene="aroF"
                     /locus_tag="KPK67_000149"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG2457174.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            30614..32404
                     /locus_tag="KPK67_000150"
     CDS             30614..32404
                     /locus_tag="KPK67_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBG2457175.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            32422..33588
                     /gene="rpoD"
                     /locus_tag="KPK67_000151"
     CDS             32422..33588
                     /gene="rpoD"
                     /locus_tag="KPK67_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBG2457176.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            33753..34442
                     /locus_tag="KPK67_000152"
     CDS             33753..34442
                     /locus_tag="KPK67_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG2457177.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEISYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            34456..35553
                     /locus_tag="KPK67_000153"
     CDS             34456..35553
                     /locus_tag="KPK67_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG2457178.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSACTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            35616..36188
                     /locus_tag="KPK67_000154"
     CDS             35616..36188
                     /locus_tag="KPK67_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG2457179.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(36248..36715)
                     /locus_tag="KPK67_000155"
     CDS             complement(36248..36715)
                     /locus_tag="KPK67_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG2457180.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(37101..37577)
                     /locus_tag="KPK67_000156"
     CDS             complement(37101..37577)
                     /locus_tag="KPK67_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG2457181.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(37697..38788)
                     /locus_tag="KPK67_000157"
     CDS             complement(37697..38788)
                     /locus_tag="KPK67_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBG2457182.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKKFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYAKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(38781..39170)
                     /locus_tag="KPK67_000158"
     CDS             complement(38781..39170)
                     /locus_tag="KPK67_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2457183.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            39362..39808
                     /locus_tag="KPK67_000159"
     CDS             39362..39808
                     /locus_tag="KPK67_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457184.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNPQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            40072..40797
                     /locus_tag="KPK67_000160"
     CDS             40072..40797
                     /locus_tag="KPK67_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2457185.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            40784..42028
                     /locus_tag="KPK67_000161"
     CDS             40784..42028
                     /locus_tag="KPK67_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2457186.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLIVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            42108..42785
                     /locus_tag="KPK67_000162"
     CDS             42108..42785
                     /locus_tag="KPK67_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2457187.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            42786..45347
                     /locus_tag="KPK67_000163"
     CDS             42786..45347
                     /locus_tag="KPK67_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2457188.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDRIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKIGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            45568..46842
                     /locus_tag="KPK67_000164"
     CDS             45568..46842
                     /locus_tag="KPK67_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2457189.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSINDK"
     gene            complement(47003..50044)
                     /locus_tag="KPK67_000165"
     CDS             complement(47003..50044)
                     /locus_tag="KPK67_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBG2457190.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYIYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            50250..50549
                     /locus_tag="KPK67_000166"
     CDS             50250..50549
                     /locus_tag="KPK67_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBG2457191.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHVYNLNSGIIDYMS"
     gene            complement(50787..53015)
                     /locus_tag="KPK67_000167"
     CDS             complement(50787..53015)
                     /locus_tag="KPK67_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBG2457192.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT        21107 a         5734 c         8716 g        17554 t
ORIGIN      
        1 attatgaaga aataggcatt ctaagaccag ttagagatat aaatggatat agaatgtata
       61 atatatcaga tatatggaaa ttaaatttaa taaaagaatt tagaagcttg aactttccaa
      121 tgaagaaaat aaaagaatac ctagatgaca gaagtataga gtctacgaaa aatattttaa
      181 atgaagaact cgatttaatt gataaaaaaa tagctgaatt tataagccat aaggaaaata
      241 ttataaaaag attgagttcg attgaaagtg taattcaaaa tacaaaaata gatgagatag
      301 aggttgtata tatagaaaaa agaaaagctt tagaattaaa tgctgatata aaaagggatg
      361 aagacttcga ttttttaatt caaaaacttc agaaagaata tgaagataga tttaatatac
      421 ttggaaacaa caatattggt tctgcctttt ctactgaagc aattaataaa ggtatattta
      481 atgagtttaa atctgtattt tgttttctgg aaagtaatga gaaagtatat aatatagttt
      541 ttgatgaagg atattatgta actttaaatt atactgggag taattctaat aataagatgt
      601 atatggagaa ggtgtttaaa tttatagaag aaaataatta taagataatt gctgacccta
      661 tagaaatcta caagatagat atacatgaga ctggaattgt agaggagttt gtaacggaaa
      721 ttcaagttcc tataactaaa tagctatatt tcaatggatt agatggtaaa tagaatgaaa
      781 aaatgttgat ttgctaccga tttgtcactg tgttataaaa tcggtagcaa attatatttt
      841 aaattataag atagcatgaa aaaataattg tttagggggt tataaatgtg aaatgttcta
      901 attgtggaag tgtaaatatt gaaaagggaa ttttaacaac tggtggcata tatgctgaaa
      961 ctattggatt aaaatataga ggagatggaa aattagataa agttataaat aaaacttttc
     1021 cagtacaatc tactatatat tcagatttat gtttagattg tggcgaaata gttagaacct
     1081 atataaagga taatactgat aggaattggt gtaaagataa gaaatagaat aatttttgaa
     1141 gtatattaat atttgaagtt gaattttatt aaagatacaa gtgaaaaagt ataaatggga
     1201 agaaattgtt gtatggaaga aaactttgca tataggggag attagttttt taatataata
     1261 acatttttaa atgtagataa aactaaaaag ggttctctag aaaacaattt ctatttaatt
     1321 atacttactt accaatacca tcacttgatg taaacaagat tctactacat tttctccgta
     1381 ctctagtata aaatcatcta gctgtgtatc tataaaatca caataatcat ataaactact
     1441 aatataagca gttaacatgt gtgtacattt atccatttca tctctaaaca tatatattac
     1501 tatcccccta aaaaactttc ataaaacaaa aagaacaata tctttttggc aactggctga
     1561 cataactcat agtttatttt ataagcgtta gtccctatag ctttgcgtca ctaaatttct
     1621 ctagttttgc cgatttagtt ttattctaca actaaaataa tacagtagga taagttattg
     1681 ttcaacatac ttgtcagaaa attgaaatat ttaacagtat cttaacaata tgaaatctat
     1741 ttttaactca agaaacaaac tatacatata gaatgtttaa taaaaattat atttagtttt
     1801 gtattgaagt ttttgtatta aaaagtttag ttcacaaggg ttacttggct agataataga
     1861 tgaacaaaaa tatcttgaaa aaatatatat gaatggcaaa aaacaaggga tgaattttat
     1921 atagactcat cccgtaatta tttatatatt gaccttgatt aaatatatta ttatttttaa
     1981 gcttcaattt ttatattata tttctaaagt ttctataaga ggattatcgc taaataattt
     2041 taataaattt gtttttttat atcctttagg taacttagta tatataacta cttctaacca
     2101 atccccatca aggcgtttaa tattcatgtt tataatttca atattagatt ttttaaattt
     2161 accttcaata tattctatgg aatcagtgct gttagaaatt ttaagagaaa cttgctctgc
     2221 tgtagggagt ttcatccatt tcctatctct atgaagtgtt atctgtacaa taacaataat
     2281 gatacttgaa acaataccaa ctaagtataa tccagagcca atggcaagac ctgtaccggc
     2341 tgttgcccat ataccagcag ctgtagtaag accacttact gattgatttc taataaatat
     2401 taatccagca cctaaaaaac ctataccact gataacttgt gctgcaacgc gggctgggtc
     2461 tagaccaata cctggagtac ccactatatc attaaatcca tacttggaaa caatgattat
     2521 aagtgctgaa gctaatgcca ctataaaatg agtgcgtatt ccagcttctt ttagtctatt
     2581 ctttctctca tatccaatta aagccccaca gaaaccagca gtaatgattc ttgctatata
     2641 ttcaaattga atcaatatat gatggttcat ttatctagca cccccttcta tataatatta
     2701 tatatactat aatactacat tttttatgtg aatatagtaa agtttaaaat ttatataata
     2761 gttatatatg aatataaaat gtataaacgt atattatgta tataaaaatg atagaaagca
     2821 tctaatttta aatgattaaa gagcaagctt aaataaatca aacttgctct tttaacatag
     2881 tgtatgtttt tatttgtgat agttagataa aaatttatct gtgtttttta catctgcaaa
     2941 actgttctgt agtgatgcca tgaatacttg atgtacagta tttgcattaa tttctatatt
     3001 attccaagat aaattcttcg ttgtacatgc atcgcttatt agagttatgt cgtatcgaag
     3061 tttctttgct gttcttacac ttgtatcaat acacatatgg ctcatcattc cacagattac
     3121 tagattagta atattttttt tctcaaggat actttgcaaa tctgtttcaa agaaactatc
     3181 tggtgtgtgt ttaattataa tttcttcatt atctagtgga aaaacaattt tatttatttc
     3241 tgaaccatga gtattaggaa gaaaaaaaga agctgtttta tctgttgata tatgctgtat
     3301 atgaataact ggtaaattgt tttttctaaa aaacagtagt atttttttag cattttcagc
     3361 agtttcttct gattgaaata attcacattt tccattttta aaataatcat tttgaatatc
     3421 aatcaatatt aaagctatag acatgattat acctcctatt tatttttatt ataatactat
     3481 tgtaaaaaat gtaaattact tactttttgg tatgtatagg attacttatt gtagcaaatt
     3541 cctttattat ctagaatttc ttgttgacca tgttcaagca tatattcgat tcctatttct
     3601 tgcattattt ccactgcttt taacattttt ttaccacgat tagtaagaaa atattcaaca
     3661 tgtaatggat atccagcaga acttatttta tcaataatcc caaaatcacg taactctttt
     3721 aattgttgaa tcaacatttt ttgatttatt ccataaatat tatgttgcaa ttcagaaaat
     3781 gttttattag attgtcttaa ttcaaatata aggatagttt tccattttcc tttgattatg
     3841 tcatgtacaa tttcaagagg acatgtatat tcagctctta ttttcatagc atatcacccc
     3901 cagtaaaatg ataataaatt atatcacttg tcttgtgata tgacaaatat atatgaactg
     3961 tatataaatt ttatgtttta ttaagttcta aatttcttct attgtagttc atatttagta
     4021 cataaataat tagtaagata taagtaaata aatatataac atgactatag aaatttaact
     4081 ttaaaataac ttatgataat ttagaggagg aatatatatg aatatatcaa gaaaagctat
     4141 gaaaataata gaattagcac aaaaaattgc aaataagaga ggtatttcag tagaagaagc
     4201 ttggagtgaa gcagtaacag aatataaaaa taaatacgaa catattgcat agatatttaa
     4261 aaatttagaa tatttggtag aatataatga ttttaaatga tactataaat tataaggtat
     4321 atcattatta acatagaagg aggatatctg cttgattgta aaaatagata atactttaga
     4381 aaaagaattt tggcaatatg tatcacatga agaaagttta aatttattca taattggata
     4441 cgttgagaat tatggattta gctctcaata tcaagatata tggtctcagg tagaagatgg
     4501 aaatataacc tctataatat taaaaaataa gtcaacactt ataatttata gttttaaaaa
     4561 taattttaat ataggagaaa tgaagaatca tataaaagat ttagatgtag aaagcataag
     4621 tggaaaaaaa tgtgtaatag atagactaat aagtgaatac aaagattttt ataaaaaact
     4681 agataataaa ttttgtgtat taaaagaaat aaaagaaata gatttttcca acatgaagga
     4741 gtataaaata gaaaatgctc aggaaaaaga tattgatgaa ataggaaaac ttttaaatag
     4801 gtcagactat aaagtaagta aaaattacat agaagaaagg aaagaacatt taaaagaggg
     4861 aaatgtaaga gcatatttta ttagaaatga tgatactatg attagcactg tgtcaactgg
     4921 tatggaaact agttttttgg caatggtggt ctctgtaagt acagataaaa gatatagggg
     4981 aaaaggactt gcaagttata tggtttacaa tttgagtaaa gaattgttgt tagaaggaaa
     5041 agttccatgt cttttttata ataatgatgt agcaggtaaa atatatcata atataggata
     5101 caaagagata aatgaatgga caattctttt taaatagcta taaaaaataa aaaactctat
     5161 cgagaattaa agtatttata aataactata tttttactat aattcatgat agagtttttt
     5221 taaatttatg atactatttt ttattattta aataatcaag tgttttaagt ttataacctt
     5281 ctttttccca atgtgtaaga acctcatcta atatctcagt atttgttgat gaatttggat
     5341 gcagtaaaac tattgcacca ggatgagttc ttgaataaat tttgtttttg gcaaattcat
     5401 gagtaggttg tttcttttca taccaatcta cataagcaaa actccaaaat attgatttat
     5461 atcctaaatc ttgtgtatat tttaaagatt gttcgctaaa tttacccatt ggaggtctaa
     5521 aatacttagg catttctttt ccagtaacat ctttgtatgc tttttctacg cttgtaattt
     5581 cttctttgaa tttttcaaaa tctgtaatac ctgccattga tggatggctt tttgaatgat
     5641 tacaaactaa atgtccttct ttttccatac gctttattaa ctctggattg ctttttatat
     5701 aactttcaac tacgaaaaat tgagctttag cattgtgttt tttaagcgta tctaataatt
     5761 ttggagtgtt tccactttca tatccagcat caaatgatag atatataact ttttcattgg
     5821 gatttcctac ataataagct ccatattttt taaaaaaatc agcttctttt ataggtgatg
     5881 gctgtttacc atcctcccta gggttaaaat accaatcata ttcatgtgtg tcgagtgttg
     5941 gactagaaat ttgagttttt gttttatcta atgatttaaa atttatacta gcaattccaa
     6001 aaagtattag agcaaatgct cctatcataa tgtatttttt taggctatct tttttcacaa
     6061 tttcacctct tatttaaaat ttaaagtata tttcttataa tatagtttga atatttatag
     6121 ttaaaaatat tcacatatac aaataataaa tgaaagaagt ttactgtaag atataaagtt
     6181 gatattaatt atcttaaata aagtgtatat aaatgataaa atataaagag aaatgattgt
     6241 aaataagtta ggctggttta acttgaaggg atataaaaat ggataatata ataagtgtat
     6301 tgaatgatgt tgttttttta gatatagaag ttagtggatt agattgttta aattcagaaa
     6361 ttttagaagt tggagctgtc aaggttaaag actggaaaat atatacctat gaatcattaa
     6421 taaaaaacaa atttgaggta cctatagaag tattctcagt atgtaaaaat ttagataaaa
     6481 atgatttaga aatagctaat gaaatagaac tggtggaaga tagacttgta aattttgtag
     6541 aagatagttt cattatatgt catgatttaa gtctgaaaaa aaagtttttt gagtatcata
     6601 tgccaaagtt aaaaaacaaa tttattgatt taattgaatt agcagttatt ttagaaccat
     6661 atcataaaga ttatagctta gaatacttaa aaaatacttt aacaaattgt aattctaaag
     6721 tggaaaatag agctttatca gatgctattg atataataaa tattgtaaat tgccttttag
     6781 taaaatttaa taactatgaa aaaactactt tggagccatt gagttttaaa ataaattcat
     6841 atcttaaaaa atttaatctt ccaacatggg aatggagcaa atttttagaa gaggcaaatt
     6901 atgatttatc aaataatata aatataaaaa aagaatataa tatatttgac tcaaaagaag
     6961 aaaagaagaa ggaaagagaa accttaaaaa ttttaaatga agaagaaaaa aattatgaag
     7021 aattattaaa atacaaaact atttgggaga ataaagaagg atttacatat gaatatagac
     7081 ctggtcaata tgagcttact aaaacaataa gagagttgtt tagaaatagc gaagatgaag
     7141 aaaaaattgc atgtatagag gcaccaactg gtataggtaa aagtgtagga tatttattgc
     7201 ctgcaatatt agaggctaga attaataaaa aaagattatt aatttctact gacacaaagg
     7261 aattgcagat acaattaata aataaagata ttccaaatgt gttagattct ttgggtctaa
     7321 atggaaaagt aagttacgga tatataaagg gaaaaaataa ctatatttgt atagatagac
     7381 tggaagcata tatagatgat tatgagtctc aaaaccctac taaaggagaa attttatctt
     7441 taatattttt gaagagatta gttgaaggtg gcaagtatgg agatatagaa gaaataaatt
     7501 atttagtgtt tgacaacttc aaggaaataa gtactcactt aagaaatgta agttgtgacc
     7561 caaatatgtg cagacctaaa aaatgcaaaa aagattgttt atataaaaac agaatagaag
     7621 aattaaaaga agaacatata acagtagtaa atcattcact attagctaaa tggccgtata
     7681 aggatgaaaa acctttagaa aatataatag tagatgaagc acataatttg actgaaaaag
     7741 gatatgattt cttttcaagc attataaatt caaagtcttt aaggtattta ttacaagaaa
     7801 tttatcctta cgagtttatt caaaatagta gctttatata taaaaaatat agtagaaata
     7861 tgagaaaaat aaaggctttt gataaatttt ataatgtatt aaaaattgga agagaagata
     7921 aacaaaaaat tgctagaagc ataaatctca ttattgaaga gatagattca atattaaatt
     7981 ttggtaattg taatgaatat aataatgtaa gtaattataa tttaagatgg gaactgaatt
     8041 tgcaaataga tgaaattgtg ggaaaactaa aaaaggatgg tatagatact gaaataagtt
     8101 atagagccta tagtgaaaag ataaagttga gttgtgagaa aataataaaa aatttagttt
     8161 caataataat aataatttat aggaatattg atgatgatag tatagataaa gaagcagata
     8221 tatataaatt tggaaaggca aagacaagag atttagagga tataaagatt atatttgaaa
     8281 tctttttaga gtatgatgaa aaggatgatt atgcaaggat agttgaaatt gataagaatt
     8341 ataatgactt tgaatttcga gtagtaccat taaaaatagc agatttattt gaagaaaata
     8401 ttttatctca attagaaaaa ggaatatttt tatcagcaac tttgagttta tctgaaagta
     8461 tgtcttattt taaaaataca ttaggaatag acagagttaa gaatgtggaa aagattatag
     8521 aaccaatttt tgattataaa aatagagtct ctgtagttgg attttctgat atttgtgagt
     8581 acagaaattc tgaatttcca aatgaaatga gtaaaattat atctaatata tcaaagatta
     8641 cagaaggtca tacattagct ctttttaata gtaaagatag acaagaaaaa acttatgaaa
     8701 tattgaaaaa atacttacat agttttaact tagaaattta tgctgataaa aaggggatta
     8761 gacaccttaa tgatttaaat agaaaatgtg tagtacttgg ctcgaaagga tgttttgaag
     8821 gtgtagacat tccaggggat ggtttggttt gtgtaacttt agacaagctt ccgaatctaa
     8881 atcctaaaga ccctttgtat tttactataa tgaagaagta tggtatagac tactatacta
     8941 taaattatcc tcaaatgacc ataaaggtaa agcaagctat ggggagaatt ttaagaagca
     9001 aatatgacta tggatgtttt gtgatttttg atgtaggaac taatataagt gtgttaaaga
     9061 gattagagaa agatttacac gattgtaaaa tttcaaaggt taatagcaat gagttttata
     9121 cttatataag gcgacattta aataaatcta gaagtttaat actaaaatct gttatatttg
     9181 atactataaa agcgttgaat gttgatgcta aaatggataa caatgatgtt gataaggata
     9241 ttataaaaaa agatataaat gaaaatataa gacaaagagc tgtaaaagga gaagtgtacc
     9301 atattgatat tataaaaaaa gatatgaaag taaaatattt tgatagaaac tatcttataa
     9361 atttagatat atttatgaga gaagaagata aaaattaaga gtgggagtga tatatttggc
     9421 aaaacaaaag ttttatgctg tgaaaaaagg taaaaatata ggtgtatata atacatggga
     9481 tgagtgcaaa aaacaagtaa atggattctc aggagctgaa tataagagtt tttctacttt
     9541 tcaagaagct aaagaatata ttgatgggtc agaaaaattg agttttcaag aagataagga
     9601 gtttatagaa gcatatgtag atggaagtta tgaacattca gtaaaaatgt atggttcggg
     9661 tgttgtgatt ttaaaaaata atgaagttat aaaaacttat agtgaaaaag gaaaagagaa
     9721 aactttagta agtatgagaa atgtagctgg agagatagag gcttctaaaa ttgcaatgca
     9781 gtactgtatt gacaataatg ttcaaaactt gattttatac tttgactatg aagggattga
     9841 aaagtggtgt actggagttt ggaaaacaaa taaagaagga actatagcat ataaaaattt
     9901 ctatgacagt attaagaata agcttaatgt aaaatttaca aaggttaaag ctcattctgg
     9961 aaataagtat aatgaggaag ctgataaatt agctaaaaaa gctattggtg tataataaaa
    10021 aaatatttat acataattac atggtataag agtatatcta aattcctact ttataattaa
    10081 acaacttgta attaaataaa ttttgattaa aaaagcagat tgtattatga aaaaataagt
    10141 atctaattta taaacaatta ataatagtat taattagata caacttattt aatataataa
    10201 aatctgcttt ttatataact ataaaatttt actacattct attagaattg cccataaata
    10261 ccttcaagtt cttcttttaa tacttcaggg tcatttatac caatagacat ggttctagcc
    10321 agtatatttg cttggtcata agttgatgtc catgagaata caccacctag acaatttcta
    10381 agaacatatt gagtttttaa gaatattgat aaagcatcat catatgacat tgcaaaatct
    10441 ccatccttaa ctaaatacgg aacttgagca gtattatcaa atctatattc atatccattt
    10501 ttatttatat aatcccttct aagctcatca taagttcttg ttatagtagc acctaatctt
    10561 ccataaaatg gtataccaag aaggattttt tcagaaggca ttccagcatt ctcaagattt
    10621 ctcaccattg catcaacact atatcctggc aaagataagt ctgaatcaaa aagatttgct
    10681 tgatgttttc taccatttgg gcctgtttca cctgctgtaa aatcataact cataagatta
    10741 aaataatcta ttataggagc tattttatct atttcagcac ttgaatttat atatcctcta
    10801 tctcctgttc cagctacact aagccattta tcatctccta taacatctct tatggcagtt
    10861 agtaagagtg taaaattttc tctatcttga ggtcttgatg ttattccaga tgcactactt
    10921 ccaggatatt cccagtctat atctattcca tctaaagcat attcatttat catctgattg
    10981 acctgtcttg caaaattata tctggatgta ggtgttaatg ctgcatcaga gaatccttca
    11041 gctccccaac ctccaatagc tacaattact tttaagctag gtttttcacc tttaagagca
    11101 agtaattgat ttaaaaatct aggataagga ataaacaaat ttccagactg gtctatctca
    11161 gcaaaagcaa atataactgc atctaatagt tgagcatcta cattagttgg atttccaaga
    11221 acatattctg ctacaattgg atttatatct ggacagtttg cattttcaac tattggattg
    11281 tatgggtctg ttatttgtgg tctagctagt ttttttgaat cttttaaagt cttcatagct
    11341 atttttttag tatttttttc actagtataa tcgtagtttt tataattatg tttatagaaa
    11401 tcttcataac tactatcttt tttatcatag ctagaattta ttttgtcgca agattcatct
    11461 ttgtcatagt taaaattcat ttcatcacaa gacttatctt taaacttatc ttgtttggag
    11521 ttgtgaactt tgttagtatt tttaatatgt tttttctcat ggtggcaatc atctttaata
    11581 tcagtcttgt gttcttgttt acagtcaaga gctttgttca ggtagtcatg atgttcatgt
    11641 tcacagttac attcattttc aatgttttta gtatgttctt ttttccagct acaagtatta
    11701 tcaattttct tgctcacttc ttcatcatta taattatctg gtacaaaaca taagtaccag
    11761 tccatacatg aatatttctt attacaactg ttaactctat tttttaattc cttatagttt
    11821 ttaggataag gtaaaatcac tggcattcca ggaaaccagt ttgcaggagt aactatatta
    11881 tctctatcac tagtctgaag tgcatctact attctaagta tttctggaat atttcttcct
    11941 gtagttagtg gataataaag aatcgttctt agaatttgat tattatctat aataaataca
    12001 gagcgaacag ttgatgtatc actcattggt tttgatatca tgccgtataa cttggcaatt
    12061 ctcatatctc tatcttctat aataggaaat ggaatttcta cacctgtaag taaagaaata
    12121 ttatacatcc aagctaaatg tgaactgtta ctatcaacac ttagaccaat tagttctgta
    12181 tttctttttt taaattcgtc ataatattta gcaaaacata aaaattctgt agtacaaact
    12241 ggtgtaaaat caccaggatg tgaaaataaa acaatccaat tacccttata gtcagagagt
    12301 ctaataggac catttgttgt attggcttta aaatcaggag cctttgaccc taaacttggc
    12361 aaatttggca tgtaaatcac ccctcaataa attatcattt attttattct attaattaat
    12421 acatattagt gttactattt tattaaaagt taagtaatcc ttatgtcaaa aaaagatatt
    12481 tattaaaaag atttaatatt ttcctaatat aaatgacaat agtaattgct atttttttac
    12541 taatttggta tagtaatgga taaggggtag tgtcaatttt agtgattata cgaggtgagt
    12601 ggattgaaaa aaattctaag aattttagtt atctccatga tgatatgttt tgcaatgact
    12661 tttgcattag tgttaataaa ttcagttatg ggagtacata taggattgga ttattacttt
    12721 ttgatgggaa gtgtgcttac agtttgttta acaattggaa ttgttttatg caatgctaga
    12781 aaaatatttg atagcaaagc tatacacaag acaagaacga aaactgtcag taataataaa
    12841 aaacaaaaga gtcattatag ggatataaaa agaaagatat cataatttat gtaaaggaaa
    12901 tatttatgta taaaataatg attgataggt aaaataaaaa cacgatttaa cttgtgtttt
    12961 tatttttttt caagaattat gatataataa ccaataagaa atttagttta ccataaaata
    13021 taaagattat taaatggaga gggaactata atgaatttta gagatttatt tgagaaggct
    13081 gtagatttta ttgatgaaaa aaggaaatct atagttgcga tttcattatc aacattaggt
    13141 gtaattgctt tgataatagt atttttctta agtagtaacg agtttagtgt aggaaatgaa
    13201 gctaatgagt tgttaaagat tatagagaaa agacagtatt ctatagctgt tgattactac
    13261 acaagtatag aaaaaaagtt ttctgattca aaaatggaaa ggtttaataa gtctgtttct
    13321 aaaaaaataa ataaattgct attaaatagt ggtgataaat atttagatgg agacataagt
    13381 caagaaagtt ttattggatt aataaatacc gtgaaagaat taaacaaaat aagtatagat
    13441 gtggatgact tattagctca agctgataga gttagagaaa tgtataaaga agaaaatact
    13501 acttatgata ttgccattaa ctacataagt actgtttcaa tattaaatgg tatgggtagt
    13561 aacctagatg tatacaaaca gaatatagaa acaatcaaag agtctagaga tgtttatgac
    13621 tcagcagtaa aagaccaaaa agcatataag tattatgaag ctattgaaaa gtataataaa
    13681 gtattaaaag aagatgaaaa gtattacagt atggctcaaa atgggaaaga acaatgtatt
    13741 gaagaaatgt atgattacta tataagtaga gctgaagagg caaatacttc tggagattat
    13801 gaaagagcat tacaatatat tgagtattta aaagacgatt attcagatga tgaaaaggta
    13861 cagagtttag aaaaaaaata taaaaagaat ttatcattgt atactttaac atctgatgac
    13921 attataaatg taatagctaa aaaaagtggc aaagacaagg caaatctatc tataaattca
    13981 cttccacaaa tgataaaaaa taataaatac tattatgctg aagtatatga gtatgataaa
    14041 ttaataaatg aagttttggt aagtgcaaaa actaaagatt tatattcata taaagatggt
    14101 aaaaaagatt acaaagtaga ttatggtgat ggatacttta gaatattaga agatggaagt
    14161 tatcaatttg gcattacaaa agataaagct aagtttgtgt taactaatac tctcgatgaa
    14221 aaagaaaata agtataaaaa gattgagata ttagatatag agaaagcaga tagatatgta
    14281 aaaagtaaaa agagtttaga agaattgttt ggaaaatata aaaatattta ttattatgct
    14341 gtagtaaata aaggcttatt tagaggcaaa gaagtttatg ctattaatat ttacaatgaa
    14401 aaaatatatg ccatatcaga aaatggacta aatgaatatt aattaataat attaaattaa
    14461 tatataatta aaaagttttt ggggttttaa ggcgcattat gtagtaatat aataataggg
    14521 atatataaat aattttattg taaggagaag tatgggatga gtaatgtgaa caaaaagcta
    14581 gttatagcta ttggagctgt agcattagta gtaataattt ttataggcat aatgtgcatg
    14641 caatttaaag gggagaaaat agcaaaaaac acatatgtta atggagtaaa tattggaaaa
    14701 ttaactaaat cgcaagcaaa acaggaatta gctaaaaaat acaaattaga gaatgttgat
    14761 tttaattaca atgataaaag ttggaaagta aagagcaaag atttaaattt atctactgac
    14821 ctggataaaa cagtagaaaa cgcatataat ttaaatagaa agagtgcttt ctttggcaat
    14881 ctatcaaaaa ctatttcagc aaattttggc aaaaaaagca acttagtagt tgcgataaat
    14941 tatgataaaa ataaattaaa agcagaaatg gaaaagatag ctaaagaaat agatgtagat
    15001 gtaaaagatg ctacacttga tataagtggt gaaaaagtca aggttatacc agattctgat
    15061 ggtcttaaaa tggatatttc taagagtatg gagaattttg ataatcaaac taaaaaaggt
    15121 aactataaaa atgagttagt agtaaaagct actccagcaa aagtaaaaaa agagcaactt
    15181 gctaatatag atacaaattt aggtacgtat tcaacaacat ttaaaacttc tcaaataaat
    15241 agaagtataa acataaaatt agcaacagat aatataagta atgtattact tatgccagga
    15301 gaaactttct catttaataa acatacaggg aaaagaagta aggaaaatgg atataaaagt
    15361 gcacctgtaa taatggaagg tgagatggaa gaagattatg gtggaggagt ttgtcaagtt
    15421 tcatctacgt tatacaattc tgtgctatac gcaggtttag aaattgttaa tgtaaaaaat
    15481 catactatac catcaagtta tgttccaaaa ggaagagatg caactgttgc agatagtgga
    15541 atagatttct tatttaaaaa taatctaaag catccagttt acataaaaaa ttacgtttca
    15601 ggaaatcaaa ttgtttgcaa tatatatgga agtgcagaag ataaacaaaa tataacaata
    15661 tcaacaaaat tagatggcgt ttcacaaact acaatgaaaa gagttaatga ccctacaatg
    15721 cctaaaggta aagaaaaagt agacaaaagt ggaagaaatg catattctgt atcaacatat
    15781 agaacattta atgatgcaaa tggtaaaaaa attaaaactg aaaaaatagc taattcatat
    15841 tatcctaaga aggaaggtat tatattagtt gggactatgg aaccaaaacc agaggaaaaa
    15901 ccaaatacag acgaaaataa gaataatcaa aatacaaaca atcaaaaccc aaataatcaa
    15961 caaaaaccag aaacaccacc aactgataat aagcctaatg agacacaacc acaaccgcaa
    16021 gcttaaagtt ctcaagaata ggatttatgt agagtaataa aaaatataaa tctaaatagg
    16081 agggattact ttgaatttaa ataatataac gaattacgtt caaagtattt gcgagaatat
    16141 ttctaatgtc ttggatgtag atgttacttt agttacaaaa gatttaacta ggatagcagg
    16201 tacaggaata ttcagaggta aaataggaga gacaatatca gagaagtcag tgtactctct
    16261 tgttttaaaa gatggaaaat cctatgttat aaataaagaa atagaagaga actgtaataa
    16321 atgtgcaaat agagaaaatt gtaaagagtt agctgatata tgcactcccg ttaaaatggg
    16381 agagcatata cttggaatct tgggtatagc tgcgtttaat gaagaacaaa aagaaaagat
    16441 actttcaaaa gataaggagt taactgcttt tgtaaggagt atgtctgatt taatatcttt
    16501 taaactagat gaactttata aacaagaagt taagactata gatgaccttg agagggaagc
    16561 tatagaaaat gccataaaga aatatggaag taatacagaa ggtatgaaaa aagtagctga
    16621 ggcattaaat ataggtatag ctacactcta tagaaaagta aaaaaattca acattaaaag
    16681 ttaatagtat tgaaaaagag gtaacctttt tatgaaaaga agaggattac cttttttatt
    16741 atgagaatgg tggaaaaata tgtatagttt tgacttgaca tattaaatat gttatcttaa
    16801 atatatattt tagagtggag gtgagtatta ttttagttaa agatataaaa caagacatag
    16861 aaagcatgcc tagaaaaatt ttagatatat ctttgataga agataataaa agtgatttga
    16921 ctttttttaa tatagaagaa gaagacggtg atgaaacatt aaaagcatta aaacagagtt
    16981 atgtgactac aaattataaa gaagaaactc tattaaactt gcaaggagct ttaaattcaa
    17041 agtcagacac aacgtctaag gaattaaaat tagaatcata taaaagatat aacaatgcat
    17101 tggatttagc atataaaaat tatataacta gtgccattaa tatggttaat aaagctctgg
    17161 agataaatcc taaagacatt gatatactaa atcttagagg tcttttaaaa ttattaaaat
    17221 gtgattttgc taaatcattt gagagttttt atactgcttt atgttatgag aataatcata
    17281 tatctagaaa atatgtaaat ctaatttcat ctgaagattt taaaactttt ttaggaagat
    17341 acaatcattc tataagattt ataaatgaag aattaaatta tgagtcaata catattttag
    17401 agaacataat tgaagaagaa cctgaactaa tagagccata tgtaatttta tctttgttat
    17461 atgataaact aggtaatgta aaaaagagag aaggatattt agacaaactt aaagagctag
    17521 ataaagataa tcctgtattt gaaaaaaatg aagaagaaaa agaagataca agtaaaaagg
    17581 aagaaataaa aaaagtaaaa agaaagaaaa gtattttgcc atatattata gttggctgtg
    17641 ttttaatagg tatggggata tatttgattc aaagcaaaaa gagaatagaa aacttgaata
    17701 accaaataag tagtaaagag gaaaagttaa gtgaaactga taaaaagtta ggagaaacga
    17761 gtaaaaaatt agaaaaaacg aataaggaat taaatgaggt taaaaataaa gaaccagaaa
    17821 gagagataac tgtagctgat gaggaagatt tatattatca agctctaagt ttaaagaaaa
    17881 acaaagaata tgaaaaagcc atagataatt ttaaaagtgt agtttcttct ggtaaaacta
    17941 aaaaatatat ttctgagtca atatatcaat tagcaataac aaataaatta ttaggaaata
    18001 aggatgaagc tataaaatat tacaaaaaat atataaatac ttatactaaa aatgaccagt
    18061 attatgatga ttcatattac gagttgggta tgctttatta tgataatggc gatttaaaaa
    18121 atgcacaaca gactttttat agtttgagaa gtgaagttcc agacagtatg tataacaatt
    18181 caaaaataaa agaaatttta agtgaaaaat aggtttatat agttaagttt tttgaaacaa
    18241 ccaagtatgt attttattaa attaaaataa aaataaattt aaaaagagat tgtgtcaaat
    18301 tataactttc tattttaaag aaagatattt gcttgttgat aaaactacaa atatatgatt
    18361 tgaagcaatc tctttaattt ttggaggtgg aaaatgaaag ttttcaatca attagtaata
    18421 attttatcta tctgggtaat aggagagtat ataagttcct ttatacaagg aattatactt
    18481 attcctggga gtatagttgg tatgctatta ttatttctac tactacaatt taaagtttta
    18541 gacttgagta gtatagaaaa tgtaagtgga tttttcctcg ataatatggc tatatttttt
    18601 attccagcag gtgtatcact tataaagtca ttagacctaa taagggaaaa tgtattagtg
    18661 cttttattag tcatatgttt aagtacacta atagtaatgt atactactgg aattattgtg
    18721 gagaaaatga taaaaaagaa ggaagaagga caaaaaaatg tataatgtat tacagacacc
    18781 agtatttggt ataatagtaa caatagtttt tttcaattta ggaagataca ttcaacgaaa
    18841 aacaagtaat cctatatgta accctttatt aattgcaatc gtaggaataa tattgttttt
    18901 aagcatatct aaaatccctt atgagaatta caagatagga gctgatagtc taaatttctt
    18961 tttaggtcct gtcacagtag tacttgcagt tccactttat aaacaatttg aattatttaa
    19021 aaagcatatg tttgagatta ttgtaggaat aggttgtggt atagtgattt cttttgtatc
    19081 tgtattgata attggcaaaa tagctaattc agatgtaagt ataataaatt cactaattcc
    19141 aaaatctata acaacaccaa tgggaatatc tttagcaaat tctttaaatg gtatagaatc
    19201 aataacagtt gtagccataa tttttacagg tatttttggt ggtatggttg cttctacagt
    19261 attcaaactg gggaatataa atcatccagt tgctaaagga attgctcttg gaacatctgc
    19321 acatgcttta ggaactacaa aggcttttga acttggagaa atagaaggag ctatgagtgg
    19381 tgtttctatt ggagtttctg gtacaattac agtaatacta attccaatta taatgaattt
    19441 tatataaaaa ataaaattaa gatgagtacc ttgaatactt aaaacaaaat aaaataaaaa
    19501 aaatttaaaa taaaaactga acgaaaaaaa tgcaaataat gaaataaaaa aatcatgtac
    19561 tgaaattata aaaagaaaaa tttcatttag gaattaatta taaagagaca attactaaac
    19621 ttgaatttgc taaaataaaa taaattactg cgaaaataga tatttagaaa aaaatactat
    19681 aaatttacta tattgcaagt taaataataa gcagtatcat attatgctag aaaaagtttc
    19741 atttaaggca taaatgagat gatttgaacg tttttaaaga ttagaatata aaagattgca
    19801 ctagacatat atgaaaaaga taatataata gatgatgtaa tagatgacag ttcaagaaat
    19861 tataaaaaaa atttatttga aatattatct attacatcag gaataatata tgtaagagta
    19921 agcttttaaa attctaaaga tttagaaaga ttgaagggag aaagaaatga gtgaagttaa
    19981 gaatacccta caaaaacatc cagttttaag atattcaaca atgctatttg ggatgttgat
    20041 gacatctata ggaataaatg ggttcttaag accagcacat ctacttagtg gaggtgctac
    20101 aggtatagca acatctataa actatttgac aaatataaat gtaggtttat taacattttt
    20161 aatcaatatt ccaatattta tcttagggtt catatacctt gaaaaagagt tttgtatttc
    20221 aagtttggta aatatgattg tattttcatt attattagga gcaactcagg aaataagtaa
    20281 tattatacct atacatgata tattgcttca aagtgtatat ggtggtatat tatctggttt
    20341 aggagtcgga gttgtattta gaactaggtc ttctcaaggt ggtacagata taatagctgc
    20401 aatactaaaa attaagaaga atatagaaat gaaagacact gctttagcaa taaatggctt
    20461 gatagtactt acaggaagtt ttttatttgg gttggattta gcattataca ctcttatagg
    20521 tctatttcta aatgcgtatt caatgagttt tattaaagat gcaatgaatt accaaaaatc
    20581 tgttatggta atgtcaaatg aagtcgattt aatagcagaa gatataatga agagtttagt
    20641 tagaggtgta acttttttag atgcagaagg tgcatacact catcaaaaaa agaagattat
    20701 atatacaata gtttcatcaa atgagatacc aaagataaaa gatatagctt tgaagtatga
    20761 taaaaaggcc tttatatctg taaatgatgt tacagaagta aagggaagag ggtttaaagc
    20821 aaaagattta tagaaaattt tacaagctgg gagtttataa tgtaaatttg tatattatat
    20881 ctctcagctt atttaaattt agtatacgaa aataaaggtt ttattaatga tttgtgtatc
    20941 actttaaaag atatcatttt aaaattaaaa ttgaaaaaat tacttaatat gtagaaaaca
    21001 tgaccactat taggatataa taaatttaat agatttagta aaatatctag gaataacgtt
    21061 ggaggaatgg cattatgaat ttatctaaag aatatgttaa tttgtatgat aattatttga
    21121 aatatataaa tgaagtaaac aaagagatta gaagtataaa gaacatgaag aaacgtgttt
    21181 taaaaagggg cgattttgct ccggaaaatg aagtattaga attggaagga aaaacaatac
    21241 gcgatataga tgatgtcgat acaaaaaaac caaaaaataa aatctataaa ttttcaaatg
    21301 gggatgtata tgtagggaaa ttcttagagg gaaagatgga ggggaaaggc tcatatactt
    21361 tttttgtaga tgaaggaaca gttatggagt acataggaga atttaaagaa gataaaaaaa
    21421 atggaaaagg aaactttata ttttcaaatg gaaatgaata tataggttat tttgaggaag
    21481 atatgatgag tggtattgga aatatgttat atagtagtaa agatgaatat attggaactt
    21541 ggaaaaacgg aaaaaaagat ggaaaaggta tttataagtg gagtgatgga tgtatatatg
    21601 caggtgaatt taaaggtgga aagatggaag ggtacggtat ttgctataat agtaaagggg
    21661 aagtacttta tgaaggagaa tggaagaata atttgattca tggtaaggga acttatatat
    21721 gggaaaaagg taaaaagtat ataggtgaat ttatgcatgg taaaaaacat gggcaaggaa
    21781 ctttttatct aaataatgaa ttggtttatg aagggacttg gaaatttgat aagccatcta
    21841 tatttgatag aacattagat gaaatatttt ccttgagact gtagctttat ctaattagta
    21901 gataaagctt ttttctaata cttactgtaa ataggtatga atgatattga ttagtataca
    21961 ctatcaatat taacttaata aatgcaatat taaaaaattt tgattgattt taagaaggag
    22021 agatatattg aagagattaa ttgctttagt gttagtagta cttattgttt tattttatag
    22081 tcctgaatta ttaaaagaag gatatgattt aaaagaaaag ttttttcaaa gtgaaatatg
    22141 gaatgaattt ttagacaaaa taaatataaa tgataattct aagaaagata aaaaagaaaa
    22201 aagtcaaaat ttacaatcaa ataatactga aggggagaac ttaggagagt cagatataaa
    22261 gaattttgaa aaaataagct taggagataa tttaagctat gttttatcaa gtatcggcaa
    22321 acctggtaga attgatatta gtgaatatgg ttttgattgg tatgtatata atcaatatgg
    22381 aaaagaattt gcaatggttg gacttgaaaa tgatgaagta gtagcacttt atagtaattc
    22441 tattaattca tgtgaaaatc aagatataaa actaaatcaa gatagacaaa cagtgagaac
    22501 taagattaca ccattaaaat ataagagaaa aggaaatacc agatatataa taaactcaga
    22561 aaatcaatat gatataataa gtaaggaagg taagtatatt acaattttct acgatattca
    22621 tgaagaaaat agagtttgtt catatttaat aattgataaa agcactgaag atgagtttaa
    22681 aaatttatat ccagatgata gcgaagaact taaaaaatgt tttgaacttg aagttataga
    22741 ccttgtaaat agtgttagaa atcaaagagg gttaaactca ttgagataca gtgaacaggc
    22801 aactttgagt tcaagaaaac atagtgaaga tatgagagat aataattttt ttgaccatgt
    22861 aaataagaag aatgaaactc catttgacag aatgaaaaga gaaggtatag tatatacttc
    22921 agctggagaa aacatagcag caggacagat taatgcaata tatgcgcatg aagcttggat
    22981 gaactctgaa ggacatagaa aaaatatttt ggggaactat aataatattg gagttggagt
    23041 aatatttgga ggaagctata aaacatatta cactcaaaat ttttataaat aactaaaata
    23101 tttgaaagga agttaaaaaa tgacatcatg gaaaaaaaag acagtatata agtgcttaat
    23161 agcagtagtc ttgttttgtg gaatagtgtt aatatctaat tttagtaaag tttctgcttt
    23221 gatgatggat actaatggaa atgttttgat taaacatggt tcaagggaaa aaaagctaat
    23281 agcaattacc tttgatgatg gtcctcatcc taaagaaact agtcaagttt tagatgtatt
    23341 aaagaaatac aatgtaaaag ctactttttt tatagctgga aaacatgcaa aatggtataa
    23401 agaaccatta gtaagagcaa gtaaagaagg tcacgagata ggaaatcata ctttcaatca
    23461 tcctgatata agtaatttaa gttctagtca gatagaagag gagattgtta agtgtgaaga
    23521 cattttgaaa gaagtaactg gaaaaaaacc gacattattt agaccaccat ttggaagtta
    23581 tagagaaaaa gaattaatag aaattgcaaa aaagcatgac tataaagttg ttttatggac
    23641 aggtgtagat gtgaaagatt ggaaaaatcc aggagctaat agcattgcag ataaaataat
    23701 taataaagta caaaatggag atataatact tttgcatgat tatgcaacta atgatacagt
    23761 tgaagcttta gatatgttta ttccaaaaat gattgaaaaa ggttttaaat ttgtaacagt
    23821 ttctgagttg ataaaataat ttttaggggg gagatttaca gtatgattct tatgatagac
    23881 aattatgatt cttttgtata caacctagtc caatatatag aagaattagg ggaaacagtt
    23941 gtagtaaaaa gaaataatga aatcaaaata agtgacattg aaaaactaaa tccagaggta
    24001 attgtgttat ctccaggacc atgttctcca aaagaagctg gtatttgcat agatattgtt
    24061 gagcatttta aggggaaaaa acctatacta ggaatttgtt taggtcacca aactattgga
    24121 catgtttttg gaggagatat tataaaagca caacagcctg tgcatggaaa agtatatagt
    24181 atcaaccata ctaataaagg agtttttagg ggacttaaaa atcctttaaa tgtcactagg
    24241 tatcattcac taatagttga ctccaataca gttccaaaag aattagaaat tacagcaatt
    24301 actgataaag gggaaataat ggggattagg cataagaaat atttaataga aggagtacag
    24361 tttcatccag aagctatttt gtcagagtat ggtcatgaaa tgcttaaaaa ttttataaca
    24421 gaagctagag aaagggtaca tatgtaatat gataagagag ataaatacta aattaaattc
    24481 ttttgagata tttactattt ttagaaatga acatgacagt tttatattag acagtgctat
    24541 ggataaagaa aaattgggaa gatattcttt tataagtagc caaccattta aggtattaaa
    24601 gtataaagat actgatgaaa atcctttaga agttttaaaa gaagaactgc ataagtatag
    24661 ggttgtaaat gatactaatc ttccatttgt aggaggtgct gtgggatatt tatcttatga
    24721 tttaggtaat tacatagaaa atttacctag aactgctgta gatgatattg agatgcctga
    24781 tatgtatttt ggattttata atcatgtaat agttatagac catcttgtgc aaaaaactta
    24841 tatagctact ccaaacatag atatcgaatt agaagaaaag ataatagatg atatagaaca
    24901 aagaatatta aaagaagaga aaaaaggtat agatagcata tgttatgaag aaaaagaagt
    24961 aacacctata agacttaaat ctaattttac aaaggaagaa tttaaaaatg cagttcaaag
    25021 tgttagagag tacataagac aaggagatat atatcaagct aatttaacac aaaggttcag
    25081 tggagaaaca gaacttacaa gctttgaatt gtatagggac ttaagaagat ttagcccagc
    25141 accatttggg gcattcttaa actttgaaga tacacatata ttatcaaatt ctccagaaag
    25201 atttataaga tgtgttaata agagaataga aactagacca ataaaaggta ctcgtccaag
    25261 agggaaagat aaagaagaag atttaagact tcaacaagaa cttagaaata gtgaaaaaga
    25321 tagagctgaa ctacttatga tagtagattt agaaagaaat gatattggca gaatatctaa
    25381 aactggaagt gttaaagttc cagagctatt tgtaattgaa ccatatgcaa atgtaaatca
    25441 cttagtttcc acagttgtag gtgagttaaa agatgataaa gatgctactg atgtaataaa
    25501 agcaactttt ccaggggggt ctataacagg agcacctaag attagagcaa tggaaattat
    25561 agacgaatta gagccaacgc aaagaaatgt atatactggt tcaataggtt atataggttt
    25621 taatggtgat atggatttta atatagcaat tagaacaata atcaaaaatg acaaaaaagt
    25681 ctattttcaa gttggaggag gaatgacttg ggattctgac ccagatgaag aatatcaaga
    25741 aacacttgat aaagctaaat ccataatgaa ggctttgagg ggatattatg aagaataatg
    25801 taggttttga tagtagttta agtaaatttg gaattggttt gtttgaaact attagagtta
    25861 aaaatggaat agcagtagat ttagatatcc atatagaaag aatgctaagt tcaataaatt
    25921 gtttggattt agatataaac tataaaaaag atttcttgat aaatgaaatt gttacatata
    25981 taaaaaaaga aaatgtcatt aataaagcac ttagaattac tgtttttgat gaaggttata
    26041 atatatctat tagggatatt ccatataatc aagagacgta tgagaagggg tttaaattag
    26101 ctatatctcc aatagttagg gggaatagtt taattcatag acataagact accaattatt
    26161 ttgaaaacat atataccaaa aatatcgcaa ataagactgg ttataatgat ggtatatttt
    26221 taaattcaga aggtgtaata ttagaatgtt ctatgagcaa tattttcttt ataaaaggag
    26281 aaagagtgta cactcctagt gatagattac cgatattaaa tggaataata aaaaaaagga
    26341 ttatagagat ttgtgacgaa ttacatatag agttgataga aaatgaaatt aatatatcag
    26401 aaattagtag ttttgatttt gtttttgtca caaatagtct tatgggcgct ataaaggtta
    26461 cagaaattga caagataaaa tttaataaag aaaatgttgt ttttgataaa atagtgaagt
    26521 tattagaata aattaaaata ttaacaattt gttggagata aaagatgata ggttgtattt
    26581 atgaagtata caatgaagag atgactaaaa taatagaaaa ccaaaacact ttggctgtat
    26641 atttagtggg ttctagtaaa gacgtagatt ttactcttta tgatgtagag ataaacgata
    26701 ttgatgtgtt cgtttttgta aaagaagggg aaaagcaaga gagaatactg tttaaaaaaa
    26761 agggaataga atttgatata aattactttt caaaagatgg tgtaaaaaaa ttattatcaa
    26821 atagggaata tttctttgtg aaagaaatga aagatgctaa agtactgttt gatagggaga
    26881 atatatcata tattataaaa gatataagta gagatattta cttagaagga cctagtaaaa
    26941 tatcattaga ggaaaagtct tttataaagc aagatattgg agcaaaaata tcgaatttaa
    27001 aaaataaaga aaaatttgat gtttttgaat atcatttttt gacgaattta tatttaaaag
    27061 atataataat tgggtacttt aatataaatg ataaatgggt accaaaagac aaaaaattgc
    27121 taaaagtttt aaaaaaagaa aataatgagc tttttgaact agtgtcaaaa gtaagtgaaa
    27181 attatgacta tcaaagactt ttagatgttt ataattatat ttttaaagaa attgagacaa
    27241 aagaggttat aaaactaatc ttatagtcac aaatcatggg gggagtttaa atgaataaag
    27301 ttgataaaga aaagatacaa catgcagtaa gagaaatact agaggcaatt ggggaagacc
    27361 cagatagaga aggtctaatc gaaactccaa atagagttgc taggatgtat gaagagatat
    27421 tctctggtct tagtgaagaa ccaagagacc atttaaaagt tctttttgca gatgaaaaac
    27481 atgaagagtt agtattggta aaagatattc cattttattc atgttgtgag catcatctag
    27541 ttcctttctt tggaaaagct catattgctt atttaccaaa aggaggaaga cttacagggc
    27601 tttctaaact tgctagagta atagatacat tggcaaaaag accacaatta caagaaagaa
    27661 tcactaaaaa tgctgcagat ataataatgg aagaattaca accttatggt gtcttagtag
    27721 tagtagaagc tgaacatatg tgcatgacaa tgagaggcgt aaaaaaacca ggttcaaaaa
    27781 ctgttacatc agcagtgaga ggaatatttg aaaaggacat tgcgtctaga gccgaggcta
    27841 tgagtttgat tactatgaag tagcaattta ttatagatat aatcatttat tgagataaaa
    27901 tttaatttaa gggagtgtca ttaattgtgt ttgattatgg taaaagaact tatataatgg
    27961 gaatattaaa tgtaactcca gatagttttt ctgatggagg agattttaat aacttggaca
    28021 tagcaataca acatgcaaaa gatatggttg accaaggtgc agatataata gacctaggag
    28081 gagagtctac acgcccaggt catagctatg tagactcaga cgaagagcta agaagagtaa
    28141 tccctgtaat aaaaaaactt aaacaggaat tggatatacc aatatcaata gacacttata
    28201 aggcagatgt tgcagaagaa gcattaaaat taggtgtcac tatggtaaat gatgtttggg
    28261 gtcttagaaa agataaaaat atggcttctg taataggaaa atatgatgca gaagtgtgta
    28321 taatgcataa tcaagatggg acaaactatg acaaagatat aatggagtca ataaaagact
    28381 tttttaaggt aagtatagaa atggcaatga gttgtggtgt aaaaaaagaa aaaatagtac
    28441 ttgacccagg tgtaggattt ggcaaggatt ttgaacaaaa tattgaagta ttgagaagat
    28501 taaatgaatt aaaagattta gggtatccta ttttgcttgg gacatccaga aaatcagtaa
    28561 taggaaaagt acttccagtt gagcctaaaa aaagattaga aggtacaata gctactactg
    28621 ttcttggtat aagagatggc gtagatatag ttagagtaca tgatgtttat gaaaatttaa
    28681 tggcagctag aatgactgat gcaatatata gaaagtaggt ataatataat ggataaaata
    28741 ttacttagta atctaggatt ctatggatat catggtgttc ttaaagaaga aaattttcta
    28801 ggtcagaaat tctttgtcga catggaactt tatattgatt caagagaagc aggtcttagt
    28861 gacgatataa ataaatctgt aagctatgcc gaggtttaca atgttgttaa agatattact
    28921 gaaaataaac aatttaatct tttggaggct ttagcggaaa atattgctga agaagtcttg
    28981 aataaattta tacttataaa tggagttatg gtcagagtta gaaagcctga ggctcctgta
    29041 aatggtattt atgactattt tggtgttgag ataaggagag caagagatga ataaggctta
    29101 tttgggaata ggtactaaca tgggtgatag atttgataat ttatcaagag catgtgaact
    29161 tttgaaaaat agtgattcta tatataaagt aaaagaatca agcttatacg aaacaaaacc
    29221 atggggatat acagaacaag cagattttct aaatatgtgt gtagaaatag aaacagagtt
    29281 tgaaccttat gagttgttag aatattgtca agaaatagag agagaactac atagagagag
    29341 aatagttcat tggggaccaa gaactataga tgtagacgta ctatttttta atgatgtagt
    29401 ttccactgat gaaagactaa caatacctca tccaaggatt caagatagag ctttcgtttt
    29461 gataccactt atggatttaa atgaagaact tataataaat gaaaaaacaa taaaagaaca
    29521 tttaaacctc ctatctgctg aagaaagaga agaagtaaag gagcttgtag gttatgaaag
    29581 aaaacctatt taataataat tcaaagatag agataaatac aaataacaaa aagataactg
    29641 taaaaaaaga taaacttata atagctggac catgcgcaat agagagttat gagcaacttt
    29701 tggagactgc taaatttgta aaaagtcagg gagcaaacat tttaagaggt ggagcataca
    29761 agccaagaac atcgccaaat tcatttcaag gattaaaaaa agaaggtctt gaaatattaa
    29821 aagcagttaa agacgaagtt ggtatggctg ttattacaga acttatggat gtaagagata
    29881 tggacgaact gtatagtata agtgatataa ttcaaattgg gtcaagaaat atgcaaaact
    29941 ttactctttt aagtgaagtg ggaaaacaaa ataaacctgt tatgttaaaa agaggaatag
    30001 catccacaat tactgaatgg ataggtgctt ctgaatatat agcaatagaa ggaaatagta
    30061 atattataat gtgtgagaga ggtataagaa cgtataatga ttatactaga aatacattag
    30121 atttagctgc agtacccatt attcaaaagg aaacaggtct tcctgttgtg gtagacccaa
    30181 gtcatgccac tggagttaga tatttggtaa aacctatgtc actggcatct tttgcatgtg
    30241 gagcagatgg aataatggta gaagtacatc ctgacccaga aaatgctttg tcagatggtg
    30301 ctcaatctct ttgctttaat gaatttgaag atttaatgaa atcaattaat aattattaaa
    30361 agaggcaaga gcctcttttt tattcgtaat cgttgctaaa gtaaatttat aagttataat
    30421 ttgatagtta gattatttaa tataaataaa gatttaatat tgatagaatt aatataaaaa
    30481 ataaaaattt tttgattaat aagaaggata tcaataaaaa aagtcgaatt gtgtatattg
    30541 agtgattgac aaatttagaa tatagtgtag aatagaaatg ttaacacaaa aaattacacc
    30601 agaaggtgat aatatgaatg atataaaaga cattattgaa gaaataaaag ctagatgcga
    30661 tatagcaagt ataatatctg aatatatgaa tataaaacaa tctggagcta attacaaagg
    30721 attatgtcca tttcatggcg aaaagacacc ttctttttat ataaatacgt caaaacagat
    30781 ttataagtgt tttggttgtg gtgaaggtgg agacgtaata aattttgtca tgaaaatgga
    30841 aaatttggat tttatggatg ctgtaaaaat tttagcaaat aaatgcggta tagaaattaa
    30901 cactaacatg aatgaagaaa ctaggattaa aatagaaaaa tctaagaaat ttcaagatat
    30961 tcatacagaa gcagctagat tttatctttc aaacttattg ggaagcaaaa atcttgggta
    31021 tgaatattta agaataagag gactagatga taaaattatt aagaaatttg gtttaggatt
    31081 ttctttggat tcatggaatt cacttatgaa tactctaatc agtaaaggat ataaaaaaca
    31141 agacttattg gagtgcggtc taattgcaaa aaatagagat ggaactaatt gctatgataa
    31201 atttagaaat agagttatgt ttcctatatt cgattataga ggaaatataa taggatttgg
    31261 gggaagagta ttagacgatt ctttgccaaa atacttaaat tctccagaca cattaatttt
    31321 taataaaaaa cagaacttat acggattaaa ttttgctaga aaaaatttag aaagtaaaac
    31381 tatagtctta gttgaaggat atatggattt aatttcgctt tatcagtatg gtataaaaaa
    31441 tgtagtagca acccttggaa cagccttgac tgaacaacaa ggcattttaa taaagagata
    31501 tgcggacact gctattatat cttatgattc tgatgaagca ggaataaaag cgactttaag
    31561 agctatagat atacttacta aattaggtat caatgtaaaa gtgttagact taaaagatgc
    31621 taaagaccca gatgaatttg taagaaaata cggacttagt gattataaaa aagctatgga
    31681 tgtttctact cactacataa aatataaaat agatcatctt aaaaaagaat ttaacattca
    31741 aaaagatgaa gaacgagtga agttcgcaaa agaagcttct aaaattataa aacaattgac
    31801 aagtcctgtt gaaattgatt tttatacaaa gtatttaagc aatcaaatag atattaatgt
    31861 cgagtctata aagagagaag tttatggtaa aaattacaat aagccatata ataacaaaaa
    31921 tcaaaagaaa atagaagaaa aagtaattga aaaagtcgaa gtcagacaag acggaaaaca
    31981 attagttgaa gaaactctaa taaaaataat gttagaagac aaaaaaatta gagaaatagc
    32041 gttacttaaa gtagaagaaa gtgatttttt attgaaagaa agtaaagaaa ttttaaatta
    32101 catgattaaa aatcaagaat tggacaaaat aactattgac aaattgaaaa gtttaaacat
    32161 atccgaagaa tacttaaaag aattaaattc aatttctttg aatagtataa acttagagaa
    32221 cacaaaagaa atagaaggaa taataacaaa tatcagaaaa aactcattag aagagcaaat
    32281 taatagcttg ttgagagaac aacaagaatt agaaaataat aatgatatga aagaggtaga
    32341 tggtagagtt atggaaattg ctttaaaaat agttgagata aataaaattt taaagagctt
    32401 gtagagaaag gaggtttgag tgtggaaaat aaatcgaata aaaaagagtt aaaaaaggtt
    32461 actgctaaga cattaataga aaaaggaaaa aagcaaggtt ctctgacact tgcagagata
    32521 atggaagctt tttcggagac tgaacttgat aaggatcaag tagaaaatct ttatgagact
    32581 ttaggaaatt tgggaataga aataacagaa acaaaaaact ataaagctga tatagatttt
    32641 tcggttgctg atgacgattt aagtataggc cacttagatg aagatgcaga ggcaatttca
    32701 catgatgact cttctgcaat agaaatagaa actgtggatt tatctttacc aaaagggata
    32761 agtatagatg accctgtaag aatgtactta aaagaaatag gaaaaattcc tctacttaaa
    32821 ccacatgaag aagtagaatt tgctagaagg atgcacgaag gtgatgagat agcaaaacaa
    32881 aggttagttg aagcaaactt aagactagtt gtaagtatag caaaaagata tgtaggaaga
    32941 ggtatgcttt tcttggattt aatacaagag ggaaatttag gtcttataaa agcagtcgaa
    33001 aaatttgact atacaaaagg atataagttt agtacttatg caacatggtg gataagacaa
    33061 gctataacac gtgctattgc agaccaagct agaactataa gaataccagt tcatatggta
    33121 gagactataa ataagctaat aagagtatca agacaactac ttcaagaact tggaagagat
    33181 ccaaaaccag aagaaattgc aaaagaaatg gaaatgacag aagataaagt aagagaaata
    33241 atgaaaatag ctcaagaccc tgtgtcttta gaaacaccaa taggagaaga agaagatagc
    33301 catttaggtg actttattcc agatgatgat gctccagccc cagcagaggc agcagcatat
    33361 tctttattaa aagaacaaat agaagatgta cttgggtcgt taaatgatag agaacaaaaa
    33421 gtattaaagc ttagatttgg tcttgaggat ggtagagcca gaactcttga ggaagttgga
    33481 aaagagtttg atgtaactag agagagaata agacagatag aagcaaaagc actaagaaaa
    33541 ctaagacatc caagtagatc aaaaaaactt agagattatt tagactaaaa agatacatca
    33601 ttgatgtttt tgtgataaat gccatgagat aagtgatatc tcatggctat tttgtagtaa
    33661 agatttaatt ttatttttgt agttatttca gtctaatatc tataacttgg attaaatcct
    33721 ataaataaag ggaattgaaa ggagaattat atttgaaatt aacagataga ttattgaaaa
    33781 tagcatcatt agtgtcagat ggtaaaaaaa tagctgatat agggacggac cacggataca
    33841 taccagtata tttgttaaaa gagggaaaag taccatttgc agtgctagcg gatgttaata
    33901 aaggacctct tgacaatgca cataaagaag tcatacagaa caatctttta gataaagttg
    33961 atttacgatt gggttctggt atagagatac ttgaaatagg ggaagtagaa gaagtgataa
    34021 ttgctggtat gggtggaatc ttgataagtg agcttctcga ggctaaaaaa gaagtagcac
    34081 ataatgtaga aaaattaata ttacagccaa tgcaagcaca agaagaactt agatattacc
    34141 ttttaaacaa cggatatgaa attttagaag aagttttagt gagagaagat tttagaatat
    34201 atgagataat tgttgccaaa tatacaggta aaaatactat aatagaagat gagatatctt
    34261 atgaggttgg aataaagctt ttagaaaaca aagattctct ttttaatgat tttatagaaa
    34321 aaaagataaa gacatatagt tctatagtta ataaattaga agggaaaaat ggggaagcta
    34381 tagataagaa gagaaaggaa agcgaagttg caataaaaaa acttgaaaat ttaataaaat
    34441 aaataggagg tatatatgct attaaagagt ctaactagaa aaattgaaaa aaagtatcct
    34501 ctaaacttag cagaggattg ggataatgta ggtcttatag ttggagattt tgatatggat
    34561 gtaaaaaagg tattggtatc tttagaagct aatgaagatg ttattgatga agctatatcc
    34621 aaaaatatag atttaattgt gacacatcat ccttttatat ttgggaaaat taataaaatt
    34681 aatagtggag atttaaaagg cagacttatc caaaaactta ttaaaaatga tatatcactt
    34741 tattctatgc atacgaattt tgatatagca tttgatggtc taaatgacta ttttatggaa
    34801 attatggaat ttggtaattc aaaggtttta gatataacta aaagcgaaaa tttgtataaa
    34861 ttagcagtat acgttccaca taactattca gatgaactta gaaaggtttt aagtaattca
    34921 ggagcaggtc atatagggaa ttatagtgct tgtacgtttt caatagaagg tgaaggacaa
    34981 tttaagccat tagaaggctc taatccgttt cttggaagtg taaatgatat agaaactgta
    35041 aatgaagtta aaattgaaac tgtagtacca cagaagcttt taggtggggt aataagttct
    35101 atgttagatg cacatcctta tgaagaagtt gcatatgatt tgtacaagtt agaaaataaa
    35161 ggtgagatat ttggactagg tagaataagt aaacttgata aaagtatgac attagaaagt
    35221 ctttcaagga aaataaaaga gaaattaaac atgaaacaca taagagtagt tggaaacctt
    35281 agtacagaca taacgaaagt agccgttgta acaggagctg gttcagaatt tgtaaaaaaa
    35341 gctaagagac aaggtgcaga agtactaatt acgggtgatg taaagtatca tgaggcacaa
    35401 gatgcacttg atattggaat gtgtatagtg gattgtggtc attttgatac agaagatata
    35461 ttcaaaaatg ttatgaagag atttttagat gaatttagtg aaattgaagt aataaagagt
    35521 aatgtatatt tgaatccatt tagtactatt tagcagtgtt tgataataaa aatactatat
    35581 aaaaaataaa tttgagttta attaggggga taatgatgaa agtagcagta atttttcata
    35641 gtgtttgtgg gagtacatat ttattagcta gagaatataa agaagcgctt gaagaaatga
    35701 atatcgaggt agatatattt agagtaagtg acgaagttgc aaaaacactt cctcaatatt
    35761 atttgataaa ttccaaagaa tacaaagacg aatttgaaag tataaatgta attaaatctg
    35821 gaaaagaaat attagattat gatgctatat ttatgggttc accaacatat tatggaaatg
    35881 tatctggacc aatgaagatg tttatggata gtttttctga tatttgggta ggtgctcctt
    35941 tgagtggtaa aatatttggt tgttttgcta cagcaggttc acaacatgga ggaggagaat
    36001 tagctttgca agctatgaat atatttgccc aacatatggg aatgactctt ttgtcagttc
    36061 catgttcagt aagaggaggt tatccagcgt atggaatact tcatatagca ggagataatt
    36121 ctgatataag accaaatgat gatgccaaga taggtataag agattaccta aaaagactta
    36181 acatataaga atatcatata aaaataccta aaagcgactg tatatacttt taggtatttt
    36241 tacttaatta taaacgtgaa gctttaccag aaatatggtc aatttctatt ttaacaacag
    36301 ttgttaagtt catatccttc tctatgtact tcatgccttc ttcaaagaat cctttagaat
    36361 atttttttat tatttctaca agagcatttt tcttttcttc attttctaca gtgcaagctt
    36421 ttccaaaaac tatagcacta gaataagtag tactaaattt atctggaata acactttcat
    36481 tagcaactac taaaaaagaa actttgttgt ttttcgatat attatctaac ttatgcccat
    36541 ttcttgcaca atgaaagtaa atagaatcat taaagtatac ataatttaca gcaactccat
    36601 aaggataacc attttcagat atagtggaaa aagtaccaaa ttcaccattt tttaatactt
    36661 caacagtatc ttcttttgtc atttctcttt ttttcaatct catttcttta aacataatat
    36721 catcctttct aattttgatg cttaaattaa attttagaac atttataaag attatgctat
    36781 gttttttaca aattttaaga aaaaaatata gtttaagata aaaggtagtg aaagagaagc
    36841 tatttggagg tttatttatg atattattgt tagagtagta taaacaatac taataaattt
    36901 aatagctggt ttcttgttat ttaaggtagt aagcatgtct ataagaaggg gaagttatcg
    36961 agggtagaag ttgttattta taacgatatt ataaagaaaa tattattata taaaataata
    37021 aatagtaaag aggtatcaag ttgaatataa tacttaatac ctcttcaaaa ttaagttata
    37081 aaagtgttgt gggaattgtc ctatacatgg taaggagata ataaagaatg tatctttaaa
    37141 gtctcaacta aacagcaaaa tttatccaac aaacatacaa taaatgcttc tttgtatcta
    37201 ggtggcttaa cagtaagtgg ccacatgtgt tttaaaatta tgtccgtttc tttttcattt
    37261 atttgaaaaa ataaagttgc attttttaat gcttctctag ggtgagtaaa tccatgtaat
    37321 ccttttctat cacctttgta atgccaatca taaagaaaaa agtcgtgtag taaagctcct
    37381 ctaataacac tttgaatatc aacacttaag tgcaattttt tacataatag ataactataa
    37441 tatgctactg acaaactatg ttcaagacat gaaacattgc catgatgagg aaatgttctc
    37501 ataagttgaa ctttttcaga agaaagtatc tcagaagcac attcattaaa atatttgatt
    37561 tcagcttttg acaacattat gaaaatctcc attcttagta atatataaat actatacttc
    37621 aacgataata tgaaatattt cttatcctac aaaatataat acccaataat acaagtaaga
    37681 taccttttat ggtattttaa agtaagagag attttattag gctaaacaat gaaaaaatta
    37741 aaagtaaagc tatagcaata tatagtattt ttccaagagg attgccaaaa tttatagtcc
    37801 atccaatacc aaacctttta ttaaccatta atgatgggtc atttggatta ttatacatga
    37861 tgcctgctat ccaatacttc tcatcttctt ctggagaata agatgagttg aattttaaat
    37921 ttggggaatt aatatatgtt gctattaaat aaattataac taaaagcgtt gtaatattca
    37981 tagcagttga tagttttgtt tttattgata aaagagtagt aaaaaactgt gttgtcatta
    38041 taatcatcaa tattaaaaac aaataaccaa ttttatttaa atatttaatg ttttcaactc
    38101 tactttcttc aattctattt gtatctatct taatccttga ctttatggat ccaatggaag
    38161 ttatatataa taaaaccatt aaacaaaagt cagtaccgat taatttaaat acattaagaa
    38221 atgatttatc tataaaaaca tcaggagacc catttatgtt ccaatgagtg gcaattgagt
    38281 ctggcaattg attataatta aatgctataa aaatactcat tcccaaagta aatagtatcg
    38341 gtattaaata gattatttta aattttttta ttattttatt tttcttattt ataaaatcca
    38401 tatctactat taattttgaa tctatatcta tatgacctaa tttagcataa atttctgatt
    38461 taagcttctt agctttttta tgtgtatgaa tatatagtat actttcatat aataaaaaac
    38521 ctataatacc aataaaatag gaaagttcca atttattaaa tgtaaaaacc agaataaaaa
    38581 ctaatataaa gtctataata aaacctaaag ataatagttt tttaaatttt ttatctaact
    38641 ttataaagta attataattt ttatgctctt ggtctataga aactccatag atgtgttttt
    38701 tacctacaaa atagggcata aaataaaatg tagcatacat caaaattaga actggaaata
    38761 ttgtaaatag acattcaatc ttactcataa tatttcacct catacttgtc aaataaactt
    38821 gaacttagtg acaaaaattc ttctttacta atacctgata aataagattt tgccaccaat
    38881 aattccaagt caagtttatt tttttgacga gttgtattat ctattttcat tggtagttga
    38941 gctacaatag caccttttct tctatcaata tttaagaatc cctcttcttt taattcattg
    39001 taggatttgt tcactgtatg taaatttaca ccaatatttt cagccatact tcttacagat
    39061 ggaagtgatt catttatttt caattctcct gaagctatag ccttagttat ttcttcttta
    39121 atttgtacat aaatagattt atcactatta aaatctaaat ttaaaaccat aaaaaacctc
    39181 cattatgctt tataaatgaa aacgattgtt atatacatag tataacataa aaaataataa
    39241 caaacacaaa atataagttt atattttgta aaaattgtaa tttaaaaaat aacataattt
    39301 ttattaaaat aatacagata ctatttatag atagaaataa aaattagaaa ggagaaaaaa
    39361 tatgttaaca tatttaatga aaagacaaat gcataaaaaa gatgatggaa tgagtaaacc
    39421 aatgatgatt acaggagctg taattacagg tgtaggagca tatgctgtat ataaaatggt
    39481 aaaaagccat aaatctaatc ctcaaatggt caatacaaat tattatggaa atagcgatta
    39541 tgatgattat aaatatgacg aatttgatta tgactgtgat tgtagagaca caaaacaaga
    39601 ttatgaatat gaagaattaa aaaaaaaagt aaaagaattt aattcaagaa gaataaattg
    39661 tgaaaattgt ccacatgttt ctcaagaaga aatgatgcaa tatgtaaata gtgttaaaga
    39721 tgtaaaggaa gagattgatt tacatgaaga tgctaaaaaa gataatattt acaaagaaga
    39781 tgaatacaaa aaatatgaag aagaataagt aatattttgt ataactgaac aaatagtaat
    39841 gtcaaattat ttaatatcta gttttaatat gtaagttgta acttaacttg aaagaatcag
    39901 tatcattctt tagatagtta agtaacaact tacattttat ttttataaat tttaatagtt
    39961 ctttgtgact attttgtaat tttagaaata ttttagtatc cagggtaatt gtgtataata
    40021 aaacttgtat agatagatag tatgaaatta agcagatagt ttaaaattaa aatgaaaaga
    40081 aaggaaatag ttatgttaaa gttgttgata gttgaagatg atagtactat atcttttgga
    40141 ataaagtatg ctttagaaca agaaggattt agtatagata tatcaaagga tttatctagt
    40201 ggaaaagaaa tgatttcttc taatgaatat agcatgatac tgttggatgt tacattgcca
    40261 gatggaactg gttatgagct ttgtcaatat ataagaggat tctctcaggt tcctataata
    40321 ttcttgactg cttgtgatga agaagtaaat attgtaatgg gtctcgatat aggtggagat
    40381 gattatataa caaaaccttt tagaattaga gaattaatat ccagaataaa agctgtttta
    40441 agaagaaaag gtaatacttc agaagaaaat aagaaaatac ttaaatttgg agatttaagt
    40501 atatatacat tagaagctag agtatataaa aatgataaag aaatatttct gacatcagtt
    40561 gaatataaac ttcttttaat tcttatacag aataaaaata ctgttttaag tagaactcaa
    40621 atattggaaa aactttggga tgtaacttat gattttgtaa acgacaatac tctaactgtt
    40681 tatataaaaa ggcttagaga aaaaataggt gatgatttat gtgaaccaat ctacatagaa
    40741 actgtaaggg ggattggtta taaatggata ggaagtgaaa atagtgtttc tatataatcc
    40801 agaagtgaag aagtttttaa gtaagtatgt tactttgatg tttattgtta tcataatatc
    40861 tattggattt agcataatca acgtgtcctt aactaaagag atggttgtaa gaaataatca
    40921 ggctataata ggaactttat caagcaagta tccaaactta gaaagtcaga tagtagacat
    40981 aataactcaa gggaagtcta tggagaacat agattatggt cagaaaatat taattaagta
    41041 taattatgat aaatctatta ggataaactc tgagcctatt atttctaaat tagttttaga
    41101 gactatcaaa ataaatataa tattagtatg tataatattt attttgatat ttgtgttgat
    41161 tgttagatat tttacgtcaa tttacaatga tttatcagat atgactaagt atgtttacta
    41221 tagttcagag ggtaagccat ttgatatgaa aaacaagaat caagaagggc agataggtct
    41281 tctaaaaact gaactcttaa agatgacgac aattcttaat gaaaaggttg aacttttaaa
    41341 aagagaaaag atatttttaa acaatacaat ttccgatata tcacatcaat taaaaactcc
    41401 tatgacttct ttaataatgt tgaatgattt attatataat gatgtaccat acgaagtaaa
    41461 aatcgatttt ttaaataaga taaaaaatca attaaataga atggattggt tgattaaaag
    41521 catgcttaaa ttatcaaagg tagaagcaaa agtgataaat tttaaaaaag ataaggtaaa
    41581 atttagtgaa ctcatacata gagcaatgct atctatgaaa atacctatgg agattaaaaa
    41641 tcaaaaactg actatagagg gaaatgataa tgtatcttat attggtgata ttgattggtc
    41701 cgtggaagca ttagtgaata taatcaaaaa ttgtgtagaa catactcctg aatttggaaa
    41761 tataattata acctataaag aaaatccatt attttctgaa ctaataataa aagatgaggg
    41821 tgaaggtata gataaaaaag atataccaca tgtatttaaa cgattctata gaggaagaag
    41881 tagttcaaaa gaagatagtg taggaatagg tcttgcaatg tcaaaatcta taatagaaag
    41941 ccaaaatggc gatatatatg taaacagcga aaaaggtaag ggtacagaat ttcatataat
    42001 atttcataaa atgtatgata gtgactaatc tgtaatattt aattcacttt atagtaatag
    42061 ccaaaaccta atatatgaag tatagaatat aaatattgga ggtagttatg gaaattttaa
    42121 gagtagaaaa tctaactaaa agttatggta aaaatgaaac aaaagttgat gctataaaaa
    42181 atgtaagttt atcagttgaa aaaggaacgt ttatagcaat aacaggtcca agtggaagtg
    42241 gtaaaagtac cttattacat ttattaggag gagttgacag accaactagt ggtaaggttt
    42301 atataaatga tgttgatata tataatttaa aaactaagga tttggcgata tttagaagaa
    42361 gaaatatagg tcttatatat caattttaca acttaattcc agtcttaaat gtgaaggaaa
    42421 acatattgct tccagcagag cttgacaata gagatattga tagagaatat tttgatgatt
    42481 taatgaaaac attaggtcta attgatagag aaaagcacct tccaaaccaa ttatcaggag
    42541 gtcaacaaca gagaacctct ataggacgtg cattaattaa tagaccttct ataatattag
    42601 cagatgagcc aacaggtaat ttagatagta aaaattcaaa ggaaatatta gaattgttaa
    42661 aattgtcagt aaaaaaatac aaccagacac taataatgat aactcatgac aaaaatatgg
    42721 cacttcaagc tgatagaatt ataagcatag aagatggaat cataaaaagt gatgaggtga
    42781 tgtagatgaa tttatataca tcactaacta ttagatactt aaaacaaaat aaaagaagaa
    42841 ccattgtaac cataatcggt ataatacttt caacagcttt aatttgtggt ataggaaata
    42901 tatttgaaag ttttatggat tatcaaatga gagaaactat aaagaatgat ggaagtttcc
    42961 atgttacatt ttatgatgta aatagaaaaa atgtggaata tgtaacgaaa tcagctgaaa
    43021 tagaaaaaca tgcatttacc aaacaacttg gatattctaa attagaaaat agtgaaaatg
    43081 ctatattaag tataaagcaa tatgataaaa atgcaataaa tggatataaa gtttctataa
    43141 aagaaggtag atttccagct aaggaaggag aaattgttct aagtgaaagt attattaatc
    43201 ttatagatga taagttaaaa ataggagata agataacttt aaaagtaggg gatatgtttg
    43261 actcaagcaa gaaaaagatt gatagtatga catttcatga aggtgattat atagcaaatg
    43321 aaaaagatag aacttttaaa gttgttggta taattgaaaa acctgggttt gaacgttata
    43381 atggaattgc aacagctata gcctacttta atcctatgaa taattacaat gatacaatta
    43441 atgtatcagt agcagtaaga aatcctaaag atgtgtataa gataagtaat aaaataagta
    43501 aaaatatata ttcaaataaa gataaagaag cagtttctga tatgataaaa tacaatgaac
    43561 atttattgag gttacaaggt gctagtaagt attcaaatat aaatagctct ataaagtcta
    43621 taattatagc agtaactatt ttagtaatca tatgtacaat tgctacagtg tacaattcct
    43681 ttagtatttc tataaatgaa cgaaaaaaac agtttgggat attaaattct ataggtgcta
    43741 catctagtca aattaaaaga ttagttttta tagaaggtat tataattagt ttaattggta
    43801 taccaatagg actaatatct ggaacagtag ctatagactt attgtttaaa attataaata
    43861 agtattttac tgaatcggtt gttactaaga tgagtttgca gatagtgtat aatccaataa
    43921 taattattgt tagtataata atagttttgc ttacaatctt tatatcaatt ctactacctg
    43981 caatatcggc ttctaatatt tctcccctta atgtgattaa aaatactgga gagtataaag
    44041 tgggcaaaat caaaagctca agacttatta aaatgatttt taaaactgaa ggagtacttg
    44101 catataaaaa tattaggaga aatagaaaaa aatttataat aactcttttt tctttaatgg
    44161 taagtataat aatatttata tcttttagtg gctttacatt acttttacta agaggtgaag
    44221 aaattagaaa ttctcaaaga aaatatgatt tgtatttaac tgcaaagaac acagctaggt
    44281 ctgtagagga tactataagt gaactagaag atattgatag gataaaaaac tttgaattgg
    44341 caacaggaca atatgtgtct ataagagtaa gtgaaaataa aataaataaa tccaaggaag
    44401 atttaatcag gaaatattat caaaaatata agattggaga tagttatgaa tatgatttca
    44461 gtaataatga attaattttt ccaggagatt ttgcagtaaa aaacaaaata aataacttag
    44521 tacagggctc ttttaataaa gagagagcaa ttgaagaaaa tggtgttata ttagttagaa
    44581 aaagtgcatt tgaagaaaat gggaagaaag gtgtagtaga acttaccaac tataaaatag
    44641 gagatacagt aaattgtgaa tatcttgatg aaaatgcctt aagtaaaaaa gtaaaggtca
    44701 agattcttgc tataactgat gaagaacgtc taggtcttgg ttatcaaaat atggggttac
    44761 aatttataac ttatgatgaa gttgctaaaa atttaaattt aaaattaagt aaaagtctta
    44821 tatttattga ttcaggagga agcattaaga ctaagaagga aatagaagct ttggcaaata
    44881 aaaataattt taacttttat gatgaaagtg caagcaatga aagtgaaaaa caagatttaa
    44941 aggttattaa aatatttgta tatggtttta tagtagtgat atctttagtg agtgttacaa
    45001 atattctaaa tacagtaagt acaagtataa atctaagaaa aagagaactt gcagtaatac
    45061 aatctatagg ggttactcct aaaggattta ggaaaatgat atatttagaa agttttatat
    45121 atggtatact gtcattgttg tttggaatac caattagtat tggtataatt ctaattatga
    45181 ataaacttat ttcaggtgta attgaatttt ctccgattat accgtggaca gccatagtta
    45241 tctgtatagt aagtgttttt ataataactt ttatagcagg gtatatacca atgagtaaat
    45301 taaataaaga aaatataata gataatataa gaagagaaag tatatagata taaaaataat
    45361 tcttaactat ataaaatatt attaaaatca tttaagtggt ataaaatatt attatatcac
    45421 ttaaatgatt ttttatttat ataagaaatc taataataaa ttactagagg tatttaacta
    45481 tttagaaaga attggtatta tataaagtaa gagaatatat aataagaaag aatatttgat
    45541 tttattggaa aacaaaagaa aaagaaggtg attactattt ctcatataac tgcgaagaat
    45601 atgtataaaa gtttagagga aagaataaac aagtttccac aaggtgctcc accatctgat
    45661 acattataca aaatactaaa tgtactatat acagaacaag aggcgaagtt agttgctcag
    45721 cttccaataa agcctttcag agtaaaaaca gcagctaaaa tttggagcgt aagtgaaagt
    45781 gaagcatata gagtattgga taaacttgct agtaaggctc tgatactaga tatagaagat
    45841 aataaaggga agaaatatat aatgcctcct ccaatggctg gtttttttga gtttgccatg
    45901 atgagaacga gacacgatat agaccaaaaa cttttggcag agctttacta tcaatatatg
    45961 aatgtagaag aagattttat caaagattta ttttattcaa cagaaacgaa gcttggaaga
    46021 gtatacgttc aagaggaagt tttaactaat gataatgagg ttagtatttt agattatgaa
    46081 agggctactc atataattga tgaatcaact catataggta taagtatgtg ttactgtaga
    46141 catagaatgc aacatgttgg aaaagcttgt gatgctccca tggatatatg tatgacattt
    46201 gacaatgtag caaattcttt aataaataat aaatttgcaa gacgtgtgga taaaatagaa
    46261 tgtaaagaac tacttcatca agcatatgaa cataacttag ttcaatgtgg agaaaatgtt
    46321 aggaaagggg ttacgtttat atgtaattgc tgtggatgtt gctgtgaagc aatggtagca
    46381 gctaaaagat ttggaaatct tcatccagta cagacaacaa gttttatacc aaatataaac
    46441 catgaaaatt gtgtaaagtg tggtaaatgt ataactgcat gtccaataga tgcgataagc
    46501 aaggttaagg aagatggtaa agaatatata aaaattgatg aggatagatg tttaggttgt
    46561 ggagtttgtg ttagaaattg ccataaaaat agcattatgt tattaaagag agatgaaaaa
    46621 ataataactc cagcaaattc agtacataga gcagttctta tggcaattga aaaagggcag
    46681 ttacaaaatt taatttttga taataatgct ctggctagtc atagagctat gggagctata
    46741 ctgtcagcta tattaaagct tgaaccagct aaaaagattt tagccagtaa acaattaaag
    46801 tcagtatatt tagataaatt gttaagtatt aatgataaat agaaactaac ttaaaaaagg
    46861 caaagtttta gacttatcta tatcttaata aacttatcat ttaatattta aatttaattt
    46921 ttacacatat aaaacttata acttaaataa aaaaaggcta tattcaatta atattacttg
    46981 aatacagcct tttttagtta tgttaagcta tttcttttat ttgcttaaat gagttttcat
    47041 ttccatttcc accaatttgt gttacagatt taaatctcat agttttaaat agttcttttt
    47101 gattataatc tagtgagttt cctactagca ttacaggaga tttagttttt ccagccaaaa
    47161 ctccaacaga taatccgtct attaaatcat cttgtttatt cattccattt ttagcaacat
    47221 ataaattgtc tatcttagaa tccttgtaaa attcttttat tacttttgca ttagtttcat
    47281 ttctagtgct acctgatatt ctctgtggat tttgaagttt actttctata tttttagaaa
    47341 ctgtatattc tccaccgatt acataagatt tatcaatctt tttgttttta aacaagttat
    47401 ttatttcagt tatattacta ttttcattag taagtattat aggcatatca ttttgagctg
    47461 atacagctcc aatgcttact gcatcggcta atcctttttc gccattaact actgaaatct
    47521 ttgatatatt gctaatattg ttcaattctt tagcaatgtt catagaagtt tgatatctat
    47581 cacttcctga aattctagat acagagatat tgtttgattt tatagtatct aaagattttt
    47641 catttataga agcctctcca ccaactacat atacattttt aacttttaat ctctttaatt
    47701 cagccaaagt tttttcattt atttgagagc ttccagtcaa taatatagga gcgtttttct
    47761 tgtatgcaaa tggtgttgca gccaaagcat ctgcaattgc tgaatcgttt attaaaactg
    47821 cattttcagc attttcccaa cctgcttgac ttatcttaat agaagtttca tatctatcag
    47881 ttcctgtaag tgcacttgca tttacttcat cttgatatgt aacctcaaat ctagcatttg
    47941 gacttcctaa gaaaacaatt acgcctcctt ttggcaatct aactctattg ttctttgtaa
    48001 ctaaagaata gtttacagga acacctttat catcataaac agcaaaagct gaattttgag
    48061 gaagattaac ttctattttt ttatttgcga tatcgtcacc aatcttatac cattttgcat
    48121 aaccattgga ttctagttca caagtaaaag atttttctgc tggcaaatta gttatagaat
    48181 cttcactaac atacgttagt gttccaaaag ataaatactc tgtaccattt tctttatgaa
    48241 gtttaatgtc ggataaatca cgacctatta caccaggtat ttctataaaa gcatttgagt
    48301 tattctcatc cattatttta gtgttaacta tataacctgg agtttcatca gaaagactaa
    48361 aacttgcttt tacagagcca aacatatagc tttgagaagt atatttttca tccactagat
    48421 agtaaccttt gccacttctt tttttccaca cttctttgac actatttgat atattgttgt
    48481 catctacttt ttgagctaca tagtataaac ttgctgtctg acctaatcca gggacatcat
    48541 cataagagct catatttaaa tatgtgatat tatttttttc ttttacaaat tttaaacaac
    48601 tattaccttt ttcagataca aatctatctt gacctatgta tatgtattta tcttcaggtc
    48661 cattttctat ataaggagaa gatactgtta aagtaccatt atcattaaca tctactttta
    48721 tcatatttga agaagcataa agaccactat tttcttttaa agagcttggc attttaactt
    48781 gttgtggctt actaaatgtt ttatcaggtt ttatttcttt aattttacct ttttctttta
    48841 atgcagatag taagatttct tgtccaatta tttcgttaag ttgactactt ccaccagaag
    48901 aaagaactgc aactgccata ttttcatctg gaagaactat aagattactg tggaataata
    48961 aagagtctcc accttttgta agagctttta aattatactg attaaatgga taagtgttga
    49021 cacaatccca acctaaacca tatccaagta tagaatcttc tccttcaggc catagaccat
    49081 ttaaatattc tttgttttcc atagctttta ctgaagcagg agaaagaata ccatttgaat
    49141 ttttcataaa tgtttgtgca aatgtacata gattttctgc acttgaatat aaaccacctg
    49201 cacctatagc attaaggtta tcttgtggaa ctgcatcttc ccaataaggt acatatgctt
    49261 ttgctagttt tgaactatca aaactatttt ctgtagtttt tgtattttgt aagttaagtg
    49321 gattatttat gtatttatca agaaaattag taaatgacat accagatact ctttctacta
    49381 aaatttcagc taaagtaaat ccatcattac aataaacaga aaaagctcct ggtttagctt
    49441 ttaatctttg tttttgtaat tcttttaaga aattatcatg accataagaa tcattgtctg
    49501 ctaataaaat agtgttttta aaactacttc ccatcaaccc tgatgaatga tttaataaca
    49561 ttcttggagt gatttctttg tatctatcat cagccatttt aaattcaggt atatacttta
    49621 caacaggagt atctaaattt aattttccat catcaaccaa cttcataaca gctgttgtag
    49681 taaacatttt actgatagat gcgatactat acatattatc cttatttaag tttttgttat
    49741 cctgtttgct gtaaactcca ccattaccag aaatctcaat tttgccatta tcaatcaaag
    49801 catattgagc acttacagca ccatactttg atagcaaagt ttggattttc tctttagcaa
    49861 gagctttagt ttttgtgtaa gattccgcgt ttttactata atcatgtaat tctatatcag
    49921 aactttgata tttttcggaa ttatcttttg tattatcagc aaaagtaata tttggagcag
    49981 cagtaaataa catactcaat actaatgtac tagataataa tttagagagc ctttgcttaa
    50041 acatattaca ttacctcttt ccctttttaa taatttataa ttagcataac attcttaatt
    50101 attttaatca acagattatt atcaataaaa aaagctttta taagtatagc tttttatata
    50161 acagagaaaa atataaaaat aatatttgtt gttgtataaa gaataaaggg tataggtaat
    50221 atataaataa aatggaggaa ttttagacaa tgtataagaa tataaataag aagcaactta
    50281 aagaaatgat gaaaaatgaa aaggatgttt tattattaga tgtaagaact aaagatgaat
    50341 ttaaggaata taaaatagaa aatgctataa atatatcttt acaagagtta atcaataata
    50401 tagatgaaat ttacgattat aaagataaga aagtagtagt ttattgcaga agtggtcata
    50461 gaagtgttac tgcatgtaat ttgttagctg aagaaggttt tgagcatgta tataatttaa
    50521 attcaggaat aattgattat atgagttaaa taatgtaagt tatacattaa atactatgta
    50581 tagctctgct tattaagtag gaatatatct aaatatttat aagataaatt taggaaagaa
    50641 cgaatttttt cgtttttttc tatgacagtt tttgtagtaa atattagttt ttaagttgga
    50701 aaaaggtagt agctttttag ctactacctt ttaatatgaa cttagaatat tattaaaaaa
    50761 atatatttaa agacagaatt aattttttaa tgaccagtta aattactatc gccaaattta
    50821 tcattaaatg gttttataac tctatttata ggtcctttta gaagtacatt taaacaaata
    50881 gacagaatca taaatagtaa tagaactagt atatctttag ttagatttgg agcatatata
    50941 ccaccaactg cttcccttgt agcacctatt gcataggtaa atggcattat agggtttatt
    51001 ctttggaaga atgtaggtgt aacttgtata gggaatgtac caccagaaga accaatctga
    51061 acgactaata gtactattgc aattgctttt cctacatttc caaatacaga aactaaagaa
    51121 tacactataa agttaaatac catactagta aacataagca gaactatcaa taaaattgga
    51181 tgttttgctg ttactcctaa caataatata tctccagttc ctattataaa accttggaca
    51241 agagctatac ttaaaaaagt aaggcctctt ccaaagtaaa tttcataagg tttatagttt
    51301 ccatgtacag atgtagacaa tagtgctgaa ataagtaata taccaaccca tgtagaaaga
    51361 acactataaa atggtgtcat tgcagaacca taattttcaa ttggatataa tttctctgtt
    51421 ttcacctcaa caggttcttt taagaaatta gactttgtca atatatcact ttcaagcaag
    51481 ctgactaatt ttttcatatc ttcaccatta ctaattttgc ttaaagtatc aatcaaatca
    51541 tccaacattc cttttgctaa aggaagtctt tctcttatca atgatatact ttcctgtgca
    51601 tttcctgaaa gttttagaga agtagtaagt atttcttcaa cctttggaag ttttgcttct
    51661 gccttatcta aaacagtaat tatatcattt gctactttta tactgtttgc aaatatacta
    51721 tttataggtt ttgatatttt tgagtcaaaa ttatttaaaa tatttaaatt aattcttcca
    51781 ataccatttg atagacttaa aacattattc aaagcactta tagatggttg ttgtccagat
    51841 ataactttgt ttttaatatc atttaatgtt gatatagatg aatctatttt attgctagag
    51901 tcttcaagat tatctatgac atcatccaat ctattatttg atgtaagttg gtttaattta
    51961 gttaaaaaat ccaccaaagt atcatttaaa gattgcaaat tagaaagttt ttctgataaa
    52021 ttatctatca acttaggtac atcttcactg ccactattta ctgcatcaat taaatttaat
    52081 gtaagagaag aagcagaaga tgaaaggtca accattaaat ttaagtcaga cttaataaga
    52141 ggagaaagtt cgtctaaatt atcatttgta tcctctaaga atttttttaa gtcacttgat
    52201 aatagttttg tatcattcag agtcttttta attgtaggta aatcagattt aatatcttta
    52261 actgtatcag ataattttga agtagcatca gaagctaaat caacagtctt atttatatgg
    52321 tcaaatctct tttgaaggtc aataagggaa ttttcaactt ttgtcaattt tggaagactg
    52381 tgttcaattc ctgcaccaac tccgtttgat ataccaagaa cgatttcact aactgttttt
    52441 actatagtct gattaacctg tagttgtatt gtagaagcac ctttatctgt aatctttggt
    52501 gcaactgcat ttattttttc attaacagta tagataattt cgcctttttt aacatcatct
    52561 gtaattaaag atgtcaaatc ttttgtaaaa ttttttggaa tcttaagtga tgcataataa
    52621 gtaccttttt taacaccttc aagagcagtt ttttcatcta caaactgcca acctaaactc
    52681 ttgttatctt tgagttgttc aattaattgg tctcctacat taatcttttg tcctgctatt
    52741 tcatttcctt tatcttcatt tgttactgct acaagcatat tagcagtgct tccataaggg
    52801 tcccaagatg cctttatatt gaaccaagca tacagagatg gtaaaaaagc aagacctaaa
    52861 actattacaa gtacggcata attgccacgt atatctttta ggtcactttt aaaaatttct
    52921 aatatatttt ttaaaccctt tgaaggtctt aaattcttga tattattcca gtttaaaatt
    52981 tcttttaaac tattagtttt tttcaaatgt ctcacctcct aaaatatttt gataaagaaa
    53041 aaagttagat ttataatcaa ttttaaatta tattactttt tacacaaata gtaaagttaa
    53101 aattttgtct t
//
LOCUS       DAEMHW010000004        52362 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEMHW010000004 DAEMHW010000000
VERSION     DAEMHW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 52362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.004"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1134
                     /locus_tag="KPK67_000168"
     CDS             <1..1134
                     /locus_tag="KPK67_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457193.1"
                     /translation="NVDIDYINEKIISKIKPKEENIKKKEIRIEDLYLLPANIELNQS
                     SQLYNEMLDLISEKVISLIEEDVSPRDIAIISPINNSILEHQIRDSLREKNIDVFSTK
                     KDKKAVDYPYGNALVVATCIFYGYLDFIKDDEYLSFLETLLEINRIKAFKIFNETRHL
                     SIDNIDEHSQYRDILQYIEEKKKSDIKIHEFLTQFYIDKMLNLKEGKKNVGLCKKIIA
                     ESESFSESLSLLGMKKEKIFVEALKSTINDYYSVVDIEELKSKDKIVITTPYSYISSN
                     IDRSIQIWVDIGSNAWNMKIEKDISNLIVLRKSFEEKKIYTNEMEEYYKKYYLYNTVY
                     NLLLNAKKIYAFKSEYAINGYIQESILYSILLKISHKGDKNYD"
     gene            1127..3346
                     /locus_tag="KPK67_000169"
     CDS             1127..3346
                     /locus_tag="KPK67_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG2457194.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIMTNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            3455..4285
                     /locus_tag="KPK67_000170"
     CDS             3455..4285
                     /locus_tag="KPK67_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBG2457195.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            4275..7742
                     /gene="addB"
                     /locus_tag="KPK67_000171"
     CDS             4275..7742
                     /gene="addB"
                     /locus_tag="KPK67_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBG2457196.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIILALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            7742..11569
                     /gene="addA"
                     /locus_tag="KPK67_000172"
     CDS             7742..11569
                     /gene="addA"
                     /locus_tag="KPK67_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBG2457197.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVECIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKEDEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIDKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEALDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            11615..12826
                     /locus_tag="KPK67_000173"
     CDS             11615..12826
                     /locus_tag="KPK67_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBG2457198.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            12813..16340
                     /locus_tag="KPK67_000174"
     CDS             12813..16340
                     /locus_tag="KPK67_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2457199.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEQDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            16471..17724
                     /locus_tag="KPK67_000175"
     CDS             16471..17724
                     /locus_tag="KPK67_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG2457200.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            17873..18925
                     /gene="ytvI"
                     /locus_tag="KPK67_000176"
     CDS             17873..18925
                     /gene="ytvI"
                     /locus_tag="KPK67_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG2457201.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            19016..20242
                     /gene="pepT"
                     /locus_tag="KPK67_000177"
     CDS             19016..20242
                     /gene="pepT"
                     /locus_tag="KPK67_000177"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBG2457202.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            20449..21501
                     /locus_tag="KPK67_000178"
     CDS             20449..21501
                     /locus_tag="KPK67_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBG2457203.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGHNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(21878..22447)
                     /gene="yfcE"
                     /locus_tag="KPK67_000179"
     CDS             complement(21878..22447)
                     /gene="yfcE"
                     /locus_tag="KPK67_000179"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBG2457204.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIDTLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(22472..23272)
                     /locus_tag="KPK67_000180"
     CDS             complement(22472..23272)
                     /locus_tag="KPK67_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBG2457205.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            23407..24267
                     /locus_tag="KPK67_000181"
     CDS             23407..24267
                     /locus_tag="KPK67_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2457206.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            24267..24953
                     /locus_tag="KPK67_000182"
     CDS             24267..24953
                     /locus_tag="KPK67_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG2457207.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSF
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            25005..25547
                     /locus_tag="KPK67_000183"
     CDS             25005..25547
                     /locus_tag="KPK67_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457208.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDIGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            25626..26093
                     /locus_tag="KPK67_000184"
     CDS             25626..26093
                     /locus_tag="KPK67_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBG2457209.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            26801..27937
                     /locus_tag="KPK67_000185"
     CDS             26801..27937
                     /locus_tag="KPK67_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBG2457210.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            27996..28778
                     /locus_tag="KPK67_000186"
     CDS             27996..28778
                     /locus_tag="KPK67_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG2457211.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            28797..29807
                     /locus_tag="KPK67_000187"
     CDS             28797..29807
                     /locus_tag="KPK67_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG2457212.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            29826..30623
                     /locus_tag="KPK67_000188"
     CDS             29826..30623
                     /locus_tag="KPK67_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG2457213.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            30721..31566
                     /locus_tag="KPK67_000189"
     CDS             30721..31566
                     /locus_tag="KPK67_000189"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBG2457214.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            31653..32828
                     /locus_tag="KPK67_000190"
     CDS             31653..32828
                     /locus_tag="KPK67_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG2457215.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            33023..33925
                     /locus_tag="KPK67_000191"
     CDS             33023..33925
                     /locus_tag="KPK67_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG2457216.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            33939..34943
                     /locus_tag="KPK67_000192"
     CDS             33939..34943
                     /locus_tag="KPK67_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG2457217.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(35087..35314)
                     /gene="acpP"
                     /locus_tag="KPK67_000193"
     CDS             complement(35087..35314)
                     /gene="acpP"
                     /locus_tag="KPK67_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG2457218.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            35486..35911
                     /locus_tag="KPK67_000194"
     CDS             35486..35911
                     /locus_tag="KPK67_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457219.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSVTLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            35959..36165
                     /locus_tag="KPK67_000195"
     CDS             35959..36165
                     /locus_tag="KPK67_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457220.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            36347..36526
                     /locus_tag="KPK67_000196"
     CDS             36347..36526
                     /locus_tag="KPK67_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457221.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            36560..36760
                     /locus_tag="KPK67_000197"
     CDS             36560..36760
                     /locus_tag="KPK67_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457222.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            37013..38029
                     /gene="ccpA"
                     /locus_tag="KPK67_000198"
     CDS             37013..38029
                     /gene="ccpA"
                     /locus_tag="KPK67_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBG2457223.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(38152..38550)
                     /locus_tag="KPK67_000199"
     CDS             complement(38152..38550)
                     /locus_tag="KPK67_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457224.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            38762..39649
                     /locus_tag="KPK67_000200"
     CDS             38762..39649
                     /locus_tag="KPK67_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457225.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDKELDYDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEELYNINEQ
                     ESEYNYNEKNTKTRAKKKR"
     gene            39764..39944
                     /gene="ssrS"
                     /locus_tag="KPK67_000201"
     ncRNA           39764..39944
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KPK67_000201"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            40127..41344
                     /gene="cdeC"
                     /locus_tag="KPK67_000202"
     CDS             40127..41344
                     /gene="cdeC"
                     /locus_tag="KPK67_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBG2457226.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(41445..42767)
                     /locus_tag="KPK67_000203"
     CDS             complement(41445..42767)
                     /locus_tag="KPK67_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG2457227.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(42818..43612)
                     /locus_tag="KPK67_000204"
     CDS             complement(42818..43612)
                     /locus_tag="KPK67_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG2457228.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            43722..45167
                     /locus_tag="KPK67_000205"
     CDS             43722..45167
                     /locus_tag="KPK67_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2457229.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            45196..45405
                     /locus_tag="KPK67_000206"
     CDS             45196..45405
                     /locus_tag="KPK67_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBG2457230.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            45425..46819
                     /locus_tag="KPK67_000207"
                     /pseudo
     CDS             45425..46819
                     /locus_tag="KPK67_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            47058..47810
                     /locus_tag="KPK67_000208"
     CDS             47058..47810
                     /locus_tag="KPK67_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2457231.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            47836..48249
                     /locus_tag="KPK67_000209"
     CDS             47836..48249
                     /locus_tag="KPK67_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2457232.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            48253..48996
                     /locus_tag="KPK67_000210"
     CDS             48253..48996
                     /locus_tag="KPK67_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBG2457233.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            48996..49466
                     /locus_tag="KPK67_000211"
     CDS             48996..49466
                     /locus_tag="KPK67_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2457234.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            49483..50241
                     /locus_tag="KPK67_000212"
     CDS             49483..50241
                     /locus_tag="KPK67_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2457235.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIKGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            50225..51067
                     /locus_tag="KPK67_000213"
     CDS             50225..51067
                     /locus_tag="KPK67_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2457236.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(51227..52132)
                     /locus_tag="KPK67_000214"
     CDS             complement(51227..52132)
                     /locus_tag="KPK67_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2457237.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
BASE COUNT        21220 a         5425 c         9341 g        16376 t
ORIGIN      
        1 aatgtggata tagactatat aaatgagaaa ataatatcaa aaattaagcc taaagaagaa
       61 aatattaaaa agaaggagat tagaatagaa gatttatact tattacctgc aaatatagaa
      121 ttaaatcaat caagtcaatt atataatgaa atgttagatt taataagtga aaaagttata
      181 tctcttatag aagaagatgt atctccaaga gatatagcaa ttatatctcc tataaacaat
      241 agtatattgg agcatcaaat tagagattct ttgagagaaa aaaatataga tgtttttagt
      301 actaagaagg ataaaaaagc tgtggattat ccatatggga atgcattggt agtggctaca
      361 tgtatatttt atggatattt agattttata aaagatgatg aatacttaag ctttttagaa
      421 actcttttag aaataaatag gataaaagca tttaagattt ttaatgaaac aagacattta
      481 agtattgata atatagatga acatagtcag tatcgtgata ttttacagta tattgaagaa
      541 aaaaagaaga gtgatataaa aatacatgaa tttttaactc aattttacat agataaaatg
      601 ttaaatttaa aagaaggtaa aaagaatgtt gggttatgta aaaagataat agctgaaagt
      661 gagtctttta gtgaaagttt aagtctactt gggatgaaaa aagaaaagat atttgtagaa
      721 gctttaaaaa gtacaataaa cgattattac tctgttgtcg atatagaaga gttgaaatcc
      781 aaagacaaaa tagtaataac tactccttat tcatatatat catcaaatat agatagaagt
      841 atacaaatat gggtagatat aggaagtaat gcttggaata tgaaaataga aaaagatata
      901 agtaatttaa ttgtacttag aaaatctttt gaagaaaaaa agatttatac aaatgaaatg
      961 gaagaatatt ataaaaaata ctatctatac aatacagtat ataatttgtt attaaatgca
     1021 aagaaaatct atgcatttaa aagtgaatat gcgattaatg gttatatcca agagagtata
     1081 ttatatagta tacttttaaa aatctcacat aaaggagata aaaattatga ttaatataaa
     1141 ttatagagaa gaccagatac caataataaa ttacgataat ggtactatgg cagtaccagc
     1201 agtaccaggt gctggaaaaa catttataat gacaaattta gtagctaaac tattacttga
     1261 acagaaacat aagggtggta aaatattaat tcttacatat atgaatagtg ctgtaaataa
     1321 ttttaaaggt agaataagaa agattcttga agagaataaa atagatgata ctaattcgta
     1381 tgaagtaatg actatacaca gtttggctgt aaaaataata aaggaaaagc cagaagttgt
     1441 aatgttaagt gaggatttta atatagctga tgatttgcaa aaaagtatta tattaaatga
     1501 atgtataagt agatttagat ttgaaggtgg agaaagagct tttagatggt ttttaaagga
     1561 gcaaaaagat gaacgctgga aagaaataac tcttgaagcc tgggaaagag gtttttttga
     1621 atttgcagga aatgcaataa gtgaacttaa gtataaggga atttctccag ataaacttga
     1681 agaaatattg tcaagaggac acaaaggaat gcttaaaatt attttaccaa tatataaatt
     1741 gtatgataga aaattaaagc aaaatggatt gctagattat gatgatatat taattttagc
     1801 agaaaaaact ttaagcttag acgaagggct tagaaagaag tttcaaacta gatacaaata
     1861 tatatttgaa gatgagtgtc aggactcaaa tgaaattcaa ggtaatataa taaaaattat
     1921 atctagcgaa aataaaaacc tagttagagt tggagatata aatcagagta taacaggaac
     1981 attttcatca tcagacccta aattttttaa agaatttata aatagtgcag atttctgtta
     2041 taggatggat atgtcaaatc gaagttcaaa agatatactt gatttagcaa acactcttgt
     2101 caagtatgtt acaaaggaat ttagacaaga agaatgtaga catgctcttg aagatatgca
     2161 aataaaaact gtgccaaatg gaatgggata taaagaaaat cctaatccag aacattataa
     2221 tataaatgta aagtggtatg agagttggaa aattgaaata gagcaaactg ctagatatgt
     2281 taaggggata aaaaataaat atccagataa aagtataggt atattggttc catttaatga
     2341 acaagtgaca caagttgcta aagaacttag agagaataat ctaacctttg aagaattagg
     2401 accaaattct ttgaataaaa ggaaggttat aaacaacata gcatatatta ttgattttat
     2461 gttacattgt gatgatgtag aaaagttaat agttgtactt aataaagttt ttatagatac
     2521 tgataatgaa gaagaaataa agtatttttt agaagaatta aaaaactatt ctaccgaaga
     2581 aattatttat aattttgaat ttagtaagtt agataatgat ttaatagaat ttaaagaaga
     2641 aagtgatata tacttaagtt ttaaaagagg aatagatatt ataaaacaga tattagaata
     2701 tccaataata aggttagacc tcttagtttt gtttattggg aaaaaactta atttagaaaa
     2761 agaagataaa gctgtactgg attatatatc tttttatata aagtatctag tatcagaaaa
     2821 tataaatatg gatttagaag atgtatataa tttattgttt gatgtaaaga acagggtgtt
     2881 taatcatata atagatgtgg tatatgagat aaatggatat gaaccagagc ccggtagtat
     2941 aacactatgt aactatcata aatcaaaggg gatggaatgg gattgtgtgt ttttactagg
     3001 tcttgttgaa tataatttcc ctgacaatat taatcaaaag tttcagagtg ataaatggta
     3061 tctaaaagaa aaatataaaa accctatggc aattattaaa tcagaagttg aagctatatt
     3121 aaaaggaagt atttcaacag attatgctca taaaacgaag attgattcta taaatgaaaa
     3181 gattagactt ttatatgtag ggattactag agcaaaggaa atgttagtac tttcaggaag
     3241 tgcttataga gatgaaagtg atatagggaa taagagaaaa gagcaaaaac catgtatata
     3301 tttaagcaga cttaatcaac atattattga aaaaaggtca aattaatata taataagtta
     3361 ttaaaagatt gcttgaaata tatctaaaag gggataaaac aatatgtaca gtaagtaact
     3421 actaaaaaat aaagttaaga gagaagtgat atgagtgaac gataaattaa aatatttttc
     3481 ttatagtcaa aattcaatta acacatataa atcatgtcca ttgaaattta aatataaata
     3541 tattgataga ataaattgga aaaatgatga tgttggtagt agagagtatt atgaaacttt
     3601 aaaaacagga agagattttc atttgatttg tgaaagatat tttagcaata tacctcttgg
     3661 aatctatttt aatgaggatg ataaaaatag taaaaagttt ttaaagtgga tggaaaatat
     3721 aaaaaaagtt gttcctattg aaaaaggaaa aacgtatttg ccagaatatg aagtaaggat
     3781 gacattaaat ggggatataa tacaggcaaa gtatgattta gttgtaattg gaaataacaa
     3841 tatagaaata tgggattgga aaaccgagtc taaaaagatt gactataaac acgtagaaaa
     3901 tagaatacaa actattgttt atatgttttt agcaaaagaa gtaataccta aagttttaaa
     3961 aatggacata aatgttaaag atataaatat gagatattat caacctgaat ttgatgactt
     4021 accaattacc attttatatg atgaagaaaa acatgaagca aatagaaata aaattcaaaa
     4081 ttatataagt atgataaaaa acactaatta tgaagaacat acatacgaag acaatctata
     4141 caatgatata gaaaaggtat ataataaaga agaacgtatg tgctatagaa ataaaaagca
     4201 ttgcaagtat tgtgaattta ataagttatg taatggcaaa gaaatagatt atagtatttt
     4261 ggaggcggaa atttatggga cttaggtttg tattaggtag aagtggaagt ggaaaatcca
     4321 cctatatact agatgagatt aaaaaagaag ctcaaaaaaa tgaaacgaca tcaattatac
     4381 ttttagttcc agaacagtac acatttgagg ctgaaaatag agtaagtaag ctattcttag
     4441 gaaaagaaaa agataagtat ctaagagtta gggtacttag ctttaagaca ctaagtaata
     4501 tagttttttc tcaagtagga ggattgacag atgtaaatat aaattctagt ggaaaagcaa
     4561 tgatggtata tagagctatt gaagatgtaa gtgaagaatt aaatgtgttt tcaaaatcaa
     4621 aatcgcagtc tggttttgta agctcaatta cagatatgat atcagaaatg aaacaataca
     4681 atataagccc tgaaatgtta gaaaatatat ctggagaatt ggataatgaa actttaagtt
     4741 taaaattaaa agatataagt aaaatatata attcttttga agggaaattg catgaaaatt
     4801 atgtagatgc tcaagatatg ttaacttcat tggcaagtaa aattgaatta agcagttatt
     4861 tagatggagc ttgtgtttat atagatgaat ttacaggttt tacaccaaat cagtacaatg
     4921 tcataaagag tatattaaat aagtctaaaa gtgttaatat ctctttgact gtagatgata
     4981 tcaactatat tgggtacagt aaatctgata tgttttctag aacgaaattt acttattcaa
     5041 aattgactca attgtgcaac gaagaaggca taaaaatatt gccacaagta aatttaaaca
     5101 caggtgttat aaaaagattt gaaaaagtta aggaattaca gcacctagaa agattttata
     5161 atgcttatcc atataaaata tattcaaatc caactgaaaa tataaagata aaagagttta
     5221 acaatttata ttcagaggtg gaagagatag ccagagaaat tgtacattta gttagagata
     5281 agaatgtaag atatagagat ataaccattg ctacaagaga tttaaataga tatgattttt
     5341 tagtacactc tatatttaat gaatataata taccaaactt tatagataag aagagagagg
     5401 caaaaagtaa tccaatagtt attttaatta ttttagcttt agaaatgaag aatagacgat
     5461 atgggtatga aactatgttt aggtatttaa aaagtggact tataggaatt gataatgatg
     5521 atataaattt actagaaaat tatgtgttgg ctaatgggat taaaggtaaa aaatggtttg
     5581 atgaaaaatg ggattataga ataacccaaa gtctttcagg tcaagaaagt gaatttgaac
     5641 tggaattaaa agagaaaata aatgaaataa aaaatagagt attagagcct atagtaattt
     5701 tacaagaaaa attaagagga aaaaatagag taaaagaaat ctgtagatat atatatgaat
     5761 ttttattaga tataaatatg ccagaaacta tagaaagttt aatagtaaat tttaaggata
     5821 aaggggaact tgatgttgca aatcaatact cacaagtttg ggacatagta gtggacatat
     5881 tagaccaaat ggtggaactt atgggtgatg aaataatttc tttagaaaaa tttataaaat
     5941 taataacctt aggttttgat gaatatgaat taggactggt tcctcctagt atagaccagg
     6001 tacttgtaag tagtgtggat agaatgaaaa atccagacac gaaatattta tacctagttg
     6061 gaactactga tggtgtgttt ccgctgatta caaaggatag tggaatattg agtgataatg
     6121 atagggaaag tcttggaaac aaaggaatcg aagttgatat tgatagtaag actaggagtt
     6181 ttgaagaaca gtttcttgta tataaagctc ttacttcgac atctaagaat ttaaccataa
     6241 catatccaat atcagaccat gaaggtaaaa cgcttagacc atcaataatt atatcaagac
     6301 ttaagaagat atttccaaat atagaaaata aaagttattt agtggaagaa aataaaagca
     6361 ctgataaaga tatacttaaa aaaataacgg ttaaatcccc tacatttaat gaacttataa
     6421 acgtaataaa aaattatgac agtgatggtt acaatacaga agagattaat agtatttggt
     6481 tagatatata taggtactat ttaaaagatg aaatctatag ttctataaca aaaaaagtta
     6541 taaaaggtct tagttatact aatcaagttc ataagattga agagaaaaaa ataaggtcat
     6601 tgtataaaag taattctctt agcatatcta ggcttgaaaa atatgctgaa tgtccatttg
     6661 cttattttat acaatatggt ctaaaagcaa aaaagcgtaa agagtattca tttacaccac
     6721 cagatttagg aacttttatt cataatattt tagataggtt ttcaaaagaa cttttacaag
     6781 ataatttaac ttggagagat attgatgaaa aatatataga attaaagata ggtataatag
     6841 ttgatgaaat aattttaaag ataccaggat atatattaaa tagttctgag aggtataaat
     6901 accttgcata tagattaaag aatatgttga caactgctat aactataatt agtcagcaaa
     6961 taaaacaagg ttcatttgaa ccaatagatt atgaagttaa atttggcaac aatggaaaat
     7021 atccacctat aaaaatggtg ttagaaaatg gacaagaagt aagtttaatt ggacaaatag
     7081 atagagttga tgaatttgaa gaaggagaaa ataaatatat aagaataata gattataagt
     7141 caggcaataa gtccataagt ctaacagaaa tatattatgg tctacaatta caattacttg
     7201 tgtatttaga tgcaatcctt gaaagtgcaa aggatgaaga tataaatata aatccagctg
     7261 caatactata ttgtagaata aataatccaa tagcaaaatt taatgaagat aaagatgatg
     7321 aagaaataca agaagcaata ttaaaagaac ttagaatgaa ggggcttgta gtaaaagact
     7381 ctcacatagt taaagagatg gataaatcat taatagatgg agaaaggaaa aactctttag
     7441 taataccagt aggtcttact aaggatggaa atgtaggtaa aagtacttca gcaataagct
     7501 atgaggattt caaactgctt agaaaatatg taaggcatgc tataaaggat ttatgtgaag
     7561 aaatgttaag tggagaaata cgtatagctc catataaaca taaagatgga acatcatgtg
     7621 acttttgtga ttactctgct atatgtcagt ttgattcaac tataaaagat aacaagtata
     7681 aaaatttaaa taataagagt aatgaagaga taataaaaat gatgaaagga gatgttaatt
     7741 agtgagttct cctaaatgga caaaagagca attagaagtt atagaaagta gagaatgcaa
     7801 tttattggtt gctgctgctg ctggttcagg aaaaacagcc gttctagttg agtgtataat
     7861 tcaaatgata acaagtagag aaaatccaat tgatatagat aagttattag ttgttacatt
     7921 tactaatgct gctgcgtcag aaatgagaga gaggataggg gatgctatag gaaaagcctt
     7981 agatgaaaac ccagaaaata aacatttaca aaaccaactt gttttattaa ataaatctag
     8041 tattacaaca atacactcat tctgtttgga tgttataaaa tcaaactttc atagaataaa
     8101 cttagaccct aatttcagaa taggagacca aactgaatgt gctattttga aacaagaagc
     8161 aatagaggaa gtatttgaag atttatatga agaaagagat gaggggtttt taaatttagt
     8221 tgaaagttat gcagaaagag gaggagataa agaagtacaa gatattatct tgggaatata
     8281 ttcatttgca atggcttctc ctgaacctaa aaaatggttg attgactcag cagaacgatt
     8341 taatattgat gaaaattttg atttttcaca gtctatttgg gctagagcta tattagacac
     8401 tgtaaaaata gagattaatg gtctttgtct aaatatggaa agggcattaa aagaagtgga
     8461 atcaatagaa gaattagaaa cttttgcaga aaagttatcc gttgaatata aaaaaattgc
     8521 tgacatttca caagcatgta ataaatcttg ggatgaggca tataaaaaga tggcaagtat
     8581 gtcctttgaa aattatgtaa aaggagtaaa aagaatttca aaagatgctc cttcgtatat
     8641 aaaagaatca aaagaaaagg caaaaactat aagagataag actaagaagt ctttagaaag
     8701 tatagtaagt gctacattca ataaagataa tgattctatt agagaagaaa taaaatatct
     8761 ttataacata gttaaaccaa tatctagtgt tgtactgaga tttgaagagg aatatagcaa
     8821 taagaaaaga gaaaaaggaa taatagattt taacgatata gagcattttg cattaaatat
     8881 acttacagat gtagatgaaa aaggtaatat tgtaccttct gatatagcag ttggatatag
     8941 aaacaagttt tatgaaatat ttatagatga atatcaggat agtaatctag tgcaggaagt
     9001 actgcttaaa gctgttgcaa atacagaaac tccaaataga tttatggttg gtgatgtaaa
     9061 acagagtata tatagattta gacaagcaaa gccagaatta tttttgcaaa aatataataa
     9121 ctataatgat aaaaagggaa gttcccatag aaaaataatg ctttacaaaa attttagaag
     9181 tagagaagaa gttgtagatg ctgtaaatta tatatttgaa aatataatga atgagaatat
     9241 aggagaaatt gaatatacag aaaaagaaag actaaattta ggtgcaaatt ttaatgtgga
     9301 tacagacgag aaatctatta ttggtggagc aacggagata cacctaatac aaaaagataa
     9361 taaacttgat gatgatatta taaatgataa agacgataga attaataaca aagaagatga
     9421 aatagaggaa gaagaaaaat tagataatat acaacttgag gctagaatgg ttggaaacat
     9481 tatcaaagat ttaatgaaag tcaatgaaga tggaaaaatt caaaaggtct atgataaggg
     9541 aatagatgga tatagacctg tggaatttag agatatagtt attcttttaa gagctacatc
     9601 tgcatgggct ccagtgtttg cagatgaatt gatgaatatg gatataccaa cgtatgctga
     9661 tgttggagtg gggtattttg atacgattga aataaaaaca atattgtcat tacttcaaat
     9721 aatagataat ccaatgcaag atatacctct tatatctgta ttaaaatcac ctatatttgg
     9781 atttacacca gaggatttga ttgatattag agttcagagt aaagataaaa ttttttatga
     9841 ggttttaaaa agtacagcag agtatgatgg atttacagat tctcaaaatg aaaatgagtc
     9901 tgagtttata ccaagtgaag agtgcataaa caaaagtaaa gattttttaa ttaagctaaa
     9961 ggaatttaaa gaaaagtcaa tgtacatgag tacagatgaa tttatatggt acttgtacac
    10021 aaggactgga tactatgctt atgttggagc attgccaggt ggaagtcaaa gacaagctaa
    10081 tttaaaagta ttgtttgaga gagcaaaaca atttgaagaa accagtctta aaggaatatt
    10141 caactttgta aattttatag ataaattaaa aaaatctagt tctgatatgg gaagtgcaaa
    10201 aacacttggt gagaatgcaa atgttgttag aataatgagt attcataaaa gtaaaggtct
    10261 agaatttcca gtagtcatat gctctgcaat gggtaaaaat ttcaatactc aagattttaa
    10321 aaagagtatt ttatatcatc ataatttagg gtatggacct caatttgtag actatgagag
    10381 aaggatttct tttccaagta tagccaaaga agcattaaaa agcaaaataa atatagaaaa
    10441 tttatcagaa gagatgagag ttttatatgt tgcatttaca agagctaaag aaaaattgat
    10501 tataactggt tctactagaa atatacaaga tagtataaag agatggtcaa atggaattga
    10561 aagtttagat acaatatcac aatacgagat attaaaaggt aaaaattttt tagattggat
    10621 aatgccttgt gtattaagac atagggattt atctaatcta ctagaagaag taggattaga
    10681 cgcagttttt aatgttgaac ataattcaaa atggtatgga aagttgtgga ataagaatga
    10741 tattttagtg gaaaagaaga gtgatgaaga aaaagaaagt attgaagaaa ttcttgaaaa
    10801 aatagatgta gataatcctg acagtgatta ttacagtgaa atagaagaaa aattaaacta
    10861 tatatatcca tatgaattta gtacaagaaa acctgctact atatctgtta cagaaataaa
    10921 aaagatacaa aataattatg aagaagagtt aataaatact atatttgaac aaaaagtaat
    10981 attaaaaaag cctctattta ttcaaaatga agaagaaaga gaaaaaataa gtggaacaga
    11041 aagaggtaca atagtccatt tagttatgga ggctttagac ttaaaaaatg taagtagtgt
    11101 aaatgatata aaatcacaaa taagaggttt tgtttcaaaa ggtataataa cagaaaaaca
    11161 agctagtata gtaaatccat ataaaatata taaatttttt gcctctaaca taggaaaaag
    11221 aatgttgaat gctgaaatta taaatagaga aaaatcaatt tatgcacaag taaatatgaa
    11281 agacatatat atttatgaaa agcttataaa caatgatgat aaaaaacttt atgataatga
    11341 aagtgtcatg ttaagaggta tagttgatgc ttattttgaa gaagataatc aaatagtttt
    11401 ggtggattat aaaacagatt ttgtaaatga ggaaaatata aatcaaataa tagagaaata
    11461 taaaaaacag ttggatttat atgcagatat tattgaaact ttaactggta agtcagtaaa
    11521 agagaaatgt atatatttat ttggagttga tgaagctgtt tgttattagt ttttatatat
    11581 aaattaaacc taaaagatag aggaagggga aaaaatgaga tttattcata catcagattg
    11641 gcatctaggg aagtccctag aaggccattc tagaattgag gaacaagcaa agttttgtga
    11701 ggaatttata aaaatagtag aatcaaatga aatagatatg gttataattg ctggagatgt
    11761 atatgatact tctaatccac cagcacaagc agaaaaacta ttttatcaga ctgtatcaag
    11821 acttgccaat aatggtcaga ggtgtgtatt aataatttca ggaaatcatg ataatccaga
    11881 gagactctct gcaataacac ctctagcaca tgaacagggg atacttatat atggttatcc
    11941 attaagtgct acgatagaag ctaagtataa agggtttgaa ataacatatg caactcaggg
    12001 atgtacaaaa ctgaatataa atggagaaaa tattgtggtt gctacattac cttatccaag
    12061 tgaaaaaaga ttaaatgaag ttttttcaag tgaagatgag tttgagaaac aaaaaaatta
    12121 ttctgaaaag gtaggagata tatttagaag tttagaagaa aattttagaa gtgatactat
    12181 aaatatagcc gtatcacata tatttgtaat aggtggagag agtactgaat cagaaagacc
    12241 tatacaatta ggtggaagtt ttttagttga aagaaaagat ttgccagaaa aggctcaata
    12301 tactgcacta ggtcatttac ataaacaaca aaaagcttcc gaacgtttaa atgcatacta
    12361 ttctggttct ccacttcaat atagcaagga tgaaagagcg tataccaaag gggcatacat
    12421 agtggatata aaagctggtg aaaaaccaat aattgaagat gtatatttta ataattacaa
    12481 acctatagaa gtatttaaat gtaatggaat agaagaagca ctagatatat gtgagcaaaa
    12541 tcaagataga gagatttggt catattttga aataaatact gatgagataa tatcacagaa
    12601 tgaaattaaa aaaatgaagg aactattaaa agacataata gaaataaaac caataataac
    12661 atcttgttat gaacaagaaa gtgtagatat aaaagaaaag tctatggcag agttgtttag
    12721 agagttctat tcttttagta aaggtgtaga gccaaaagga gaacttatgg atttatttct
    12781 agatataata agtgaagaag gtgaatcagc agatgagacc aattagatta gaacttacag
    12841 ggcttaatag ctatattgat aaacaagtta tagactttga aaaattaatt gaaagaggtt
    12901 tgtttgggat atttggaact actggaagtg gaaaatctac aatcttagat gcaataacta
    12961 ttgctatgta tggaaatata tccagaaata caaaagaata tataaacagt gtgtgcgata
    13021 aggcaattat atcatacgag tttgagatag gaagcaaaaa cactagaaga agatacattg
    13081 tagatagaac cattgctaga agcaaaacag gaacaaagac ctctaatgct agattaatag
    13141 aagtattgaa tgacaatacc caaaatgttt tagctgataa agttgtggaa gtaaatgaaa
    13201 aagtagcaca ggttgtagga ttaacggcaa atgattttac taggtcagta gttttaccac
    13261 aagacaaatt taatgagttt ctaagattat cacgtgcaga tagaagagac atgttagaga
    13321 gaatttttaa tcttgagaaa tatggaagaa gtcttggaga aaaagtgaaa aagagaaaaa
    13381 atatacagtt acaaaattta aaagatttga aatcaaaatt aagtcaatat gatggaatta
    13441 cagaggaagt atataacaat ataaaccaag aattaataga gttaaaaaat ttggaacaag
    13501 ataagaataa tgctttagac ctagctcaaa aatcttatga agaaagtaaa acagtatatg
    13561 aagaacaatt aaagttagaa aaaaatgaac ttagaaaaaa tgagcttgat ttaaaaaata
    13621 gtgagataaa agaaaagatt gctcttgtag aaaatgatag taacgctaaa aaggtagacc
    13681 catacataag ttcagttcaa aatctagaaa aaaagataga tgaagatagc tttgcagtaa
    13741 gtaatttaga aaagaaatta gcaatattaa atcaggagct tgaagttaca aaaaatagat
    13801 atgaaaaaat aaacaaaatt aagaatgaag aagtccctaa attgagtgaa gaaaaaataa
    13861 gattgcaaca agcaataaaa ttagaagaag agttagtttt attagataga gagttaaaag
    13921 agctaaaaga aagtggaatt aatttaaata agactaaagt tgaattagaa aaagtaaagc
    13981 aggtgtcaga gtccagaaaa gatgcagtta ctaaatctat aaaagaagtt gaaggaaaga
    14041 ttgataaagt aaatataagt gcagaattaa aacaaaaaat atttctagca tatgaatacg
    14101 agaaagatta caacaacgta ttggaagaaa aaaatcaaaa gctaaataaa ctagaagaaa
    14161 tactcaaaga gacagaaaat ataaatttaa aagttagata tattgataaa gataagaatg
    14221 atgtaaatcg taatttagaa aatttatcac ttcacttgga tgttcttctt aaaaaatgtc
    14281 ctggtaagag tgctgattta cttttaaaat ctgaatatgt tactgaatta agaaataaag
    14341 caaataatac taaagaaaat gaaattaaga aaagcagtat acaagatgaa ttaaagataa
    14401 tacttgaaag taagtttaat actgaaaggg aagtaaattt actaaatgaa aaacttgaaa
    14461 ataatagaaa gaacagagat gacctagaaa aagaattaga agaactaaag tatttaaatt
    14521 tagcctctga acttagacgt gaattaaaag aaaatatgcc ttgtccagtt tgtgggtcaa
    14581 aacatcatga aaatcataat ataactaaat atgatgaaaa tatatctttt gtcaaagaaa
    14641 aactggagaa attagaaaaa gaaaaaataa gtataagaaa caatatagag gaattaaatg
    14701 ctaaagtaag tgggtattta tctatagaaa aaatgaagac taaagaactt gaagatgtaa
    14761 aaggaaaact aggggggatt ccatctagtc aattattaaa aaaacttgat gaagaacaaa
    14821 gaaaacttgc attgttaaaa tctaatattc aggagtggga gaaagaaaaa gagagtactg
    14881 aaaataaagt aacattagcg aaagaagaaa aaaataaaat tgaaaaagaa gaattaaaaa
    14941 taagagagag tctgaataat tataagaaat taacaagaga tttaaacata gaaatagaag
    15001 gtttagaaaa taaatgtaaa aaacttaaac aggaatacct aggattaaag acgattacta
    15061 aagtctctaa tctattgtca aaagtaaaag aaataaggga gaatgaaaaa gaattagaat
    15121 tactaagtac tgggcattca aatttattaa aaaatagaga ctcactagat attaaaataa
    15181 gagaaaatga gagtaaacta catgaaatag agatagaatt aataaaggct agagaattat
    15241 atgtagaaaa gaaattgagt agagataata agtataaaga agttattagc ataacgaaag
    15301 gtgatttagc taaaaatctt ttacataatg ttgaagaaaa tatatgtaag atattagaac
    15361 aagaagaatc aagtaagaaa aaattggaag agcaaagatt agaatatgaa aaaaacattg
    15421 ctgaaaagca caatatagat ggtagactta aaactgctaa ggaacaatat aaagaccaaa
    15481 aatcaacact aaataaacta ttggcagaaa ataaatttga aagtatatat gcagtaaaaa
    15541 gagctttatt agatactgat gcagtaaaaa aattgattga agaaatacta gagcatgaag
    15601 aagaacaaaa gttattatca tttaaaataa aatcatcaaa agaaaaatta aatggaagaa
    15661 gcataaagaa ggagtacttt gaacagttaa aagatgaaat ttataatttg aaagtagaaa
    15721 taggaaatat atccaaagat attggagcta atcaaaatca gttaataaca ttaaaagact
    15781 ctttagataa gataaatgat tttaataaac aacttaaggt agttgaacac aatgtggact
    15841 tattggaaga attagataaa tgtgtacaag gaaataaatt tgtagaatat gtttctacaa
    15901 accaattaaa atatatagcg ttggaagcat caaaacgatt ggatggaata acaaaaggaa
    15961 gatatgcact tgaaattgac tcaacactaa actttgtaat gagagataac tttaatggag
    16021 gagaaagaag aagcattgat actctatctg gtggagagac attcttgaca tctctatcat
    16081 tagcacttgc tctttcatct caaatacaat taaagggaag tgcaccatta gaatttttct
    16141 ttttagatga aggttttggt tcattagata gtgaactttt agaaattgtt atagaatcct
    16201 tggaaagatt gcatagtaat aatctaagtg tgggtataat aagtcatgtc gaggaactta
    16261 aaaatagagt tcctgttaag ctattagttt catctagtga ggctggaata ggttcaaaag
    16321 taaaaattga atatagttag aaaatatatg ttaattgaat atattatcta atatttccaa
    16381 ataatatgta attgtgatat aataaaaact aatattaaag ttatttataa ttaatattaa
    16441 tataaaaaca tgtaagtagg agtgtggtaa gtgttggaat cgcccaataa tttgattcag
    16501 attatttttt taatagtatt acttataggg tctgcatttt tctcagcatc tgaaacggcc
    16561 ttaatgtcgt tgagtaaaat cagaatcaga tatatgcaag atgaaggagt taaaggagct
    16621 aagttagtaa gttcattaat agaaaatcca aataagcttt taagttctat attggttgga
    16681 aataacgtcg taaatatagc agcaacatcc atatcaacat ctttgtttat aggattaatg
    16741 ggagaaaaag gagtggcact tgctacagct gttatgacag tattagtatt aatatttggt
    16801 gagattactc caaaaacaat agcagcaaat aattcagaaa aggtttctct attagtatct
    16861 aaacctataa aagcaattat atttatatta agaccaatag tatggatatt taatataatt
    16921 acaaatatta tatttaaatt atttggtata acaaataaag gagctaaatc attcataact
    16981 gaagaagaac taaaaactat ggtaaatgta agtcatgaag aaggcgtcct tgaaatggaa
    17041 gagagagaaa tcataaataa tgtttttgaa tttggagata tgcaagctaa aaatgcaatg
    17101 gtacaaagaa tagatatggt agctatagat atggaagata gttatgatga gataatacag
    17161 gtatttaaaa ctgaaaaatt aagtagaatg cctgtgtatg aagaaactat agatgatatt
    17221 gtaggaatac ttaatataaa agatattata tttttatctg atgaagaaat agagtcattt
    17281 gatattaaaa attatatgag agaacctttt ttcacgtatg agttcaaaaa gataacacaa
    17341 cttcttgaag agatgaaact tgaaaaaagt cagatggcta tagttgtaga tgagtatggt
    17401 ggaacatctg gacttttaac tatagaagat ttagtagaag tcattgttgg agatatagag
    17461 gatgaatatg atgaagaaga ggatgaaata caagttataa aagaagatga gtatattgta
    17521 gatggaagta ctaaaattgg tgatgttaat gaacttattg gtgtaaactt ggaatctgag
    17581 gagtttgatt ccataggagg ttttataatt ggacacctaa gcagattgcc agaagaaaat
    17641 gaagtaattg aagttgataa tataagattt tgtatagaaa gtatagaaaa aaatagaatt
    17701 aaaaaaataa gaatatatac ataatgtaat aatgttctta aatttattag aagtgtataa
    17761 atataaaaga tatcttttat atttatacac ttctttttat tttgtattca aataaaacta
    17821 gtatgaaatg gacaaaataa aaatataaat tagaagaaag gagattttta agatgagcat
    17881 actagaaaat aattttttat acaaattaaa aaacaacact atcttttttt gtatatatac
    17941 attgttattt atttttattt acaaagcttt tccatacata gctccatttt ttctaggtgc
    18001 tataatagca cttatgatta atcctatatc acaaaagtta gaaaataagt ttcgtataaa
    18061 taaaggtatc tctactcttg ttctaagttt tcttgcagtg gctatagtca gtacagttac
    18121 tacaataata gtaataaatt caatgaaaga attaatggga tttttaaata atatatctgc
    18181 taatcctgag gatataagta atactattat gtatttttta aataaaataa atgactttat
    18241 gaaatcattt caagaaatag ctaactttga tgtagaacaa ttagtaaaca aattcagtgg
    18301 tgaagtaatg caaattacaa aaaatcttct tactagtata cttggattgg ctacttctat
    18361 tccatatatt ataatattta taattaccct gtttattgct acctacttta tagcaaaaga
    18421 ccttgataag attgaaaata gtttttataa tatgtttact gttgatgtta gaaagaaggt
    18481 aaaaaacgta aagaaagagg cagggctttc acttgttgga tatataaggg cctatacaat
    18541 acttatggct ataacatttt ttgcaatctg gggaagtttt gctttatttg gattgaaata
    18601 tggtctaata gtagggtttg taggagcttt aatggattta attccattcc taggtataat
    18661 aacaatatat cttccagtaa ttgtttatta ttttcttata aaaaattatt ttatagctat
    18721 aagtatgact gtgatatttt ttgttttgtc tttgattaga gaaatacttg aacctaaact
    18781 agtatcagta aatgttggac taaatccatt agctacccta gcagcgattt ttattggaat
    18841 ccaagtaaaa ggaataattg gagtaatttt ttgtttgggt ttggtatgta tgcatgatat
    18901 acttaagaag gtggatattt tttaattata ataattgata gattttttta aaaataaaat
    18961 tttattcaca ggtaaataaa aaaatattaa tcttattaag aaagagggta tatacgtgaa
    19021 acaaaaagta gtggaaagat ttttaaaata tgtatcattt gatacaacat ctaattctca
    19081 gtgtgagaac tgtccatctt cagaaggtca aagagtttta gctaagtata tagttgaaga
    19141 gctaaaaaca atgggtgttg atgatgtgag tttagatgaa aatagttata taatggctac
    19201 tttaaaagga aatactgatg gtgtggatac tataggattc atatcacatt tagatacaat
    19261 tgaagatgtt agtggaaaag atattaaacc aagaataata gaaaattatg atggaaaaga
    19321 catagtttta aatgaagcgt taaatgttat tacgtatgta aaggattctc cagagttgga
    19381 agaatttaaa ggtgacgatt taatagtaac tgatggaaca actctacttg gctctgatga
    19441 taaagctggt atagctgaga tagtgactgc aatagagtat ctaataaatc atccagaaat
    19501 taagcatgga gatataaaaa taggatttac accagatgaa gaaattggta gaggtgctga
    19561 tttatttgat gttgaaaaat ttggagcaaa atatgcatat actttagatg gaggaatagt
    19621 tggagaactt gaatgtgaaa attttaatgc tgcaaatgca actattacta tacatggtag
    19681 aaatgttcat ccaggttcag ctaaaaataa aatggtaaat gctatacata tagctgctga
    19741 aatatcagaa atgttcccag cagatgaaag accagaaaca acagaaggat atgaaggatt
    19801 ttggcattta aattctatag gtggaaatgt cgagaatgta agtatggctt atattattag
    19861 agaccattgt aaagaaaaat ttgaaaatag aaaatctatt atgatagaga atatagaaaa
    19921 aataaataag aaatacgata atagagtgga attggattta aaagattctt attacaatat
    19981 gaaagaaaag attgagccag ttatgtttat agttgatata gctaaagaag ctatggagga
    20041 attaggtata aaaccacgtt tagttcctgt aagaggtggt actgatggag caagattatc
    20101 ttttaatgga ttaccttgtc caaatatatt tactggtgga ttaaacttcc atgggaaaaa
    20161 tgagtgtata ccagttagtt ctatggagaa agctactaaa ctaattgtaa gaatagctga
    20221 aaaatatgct gaaagagttt agttaaaagg tatttaaaaa ttaagcattt aaagttataa
    20281 tagctatatc attctttata agtcaagttg attataagct tgttttgtat ttaaaatggt
    20341 atagctattt ttgtaattat ttttataaaa gataattatt tttagtttag ggtatcaata
    20401 aaaagtatga atatcaatag gtattaacat ttaaaagggg gaattgtatt gaaaattaaa
    20461 tctaaaaaaa taatagcatt tgcactttca cttgggatta tgttgccaag ttgtgtatat
    20521 gcagacacta ttagtaaaaa tcaaaatgca caggtagata tgaacaatta tcttcaaggt
    20581 agaaaatctc caaaggagga aaaacttatt ggtgaagata gatttgatac agcgataaaa
    20641 atatctcaaa gtggttggaa taatggttca gaaagggtgt ttctagttaa ctcaaattca
    20701 ttgccagatg cattggcttc aactcctttg gcaagtaaat tggatgcacc aatactgctt
    20761 acaaataaaa atagtatacc atataatgta acagaggaaa taaaaaggct taatccagca
    20821 gaagttattt taattggaag tgaaggagct atttcatcaa gtgtaaaaga atctcttgaa
    20881 gatatggaag tattagtcag tagaatcggt ggagcagata gagaagaaac atctttactt
    20941 cttactagac agctagatga tactggtgat ttaggtgtct caaaagtggc agtggttaat
    21001 gggcataatg gactggcaga tgcaacttca atatcaagtc cagctgcaag tgataataca
    21061 gctataatat atacaggtaa ggatagcata agaagtgagg caaagaattt tattacacaa
    21121 aactcaacta agacatacat agtaggtggg gagtacagta taagtaaaaa acttgaagga
    21181 cagttagtaa actcagaaag actagcagga acagatagaa aagatactaa tgcgaaagtt
    21241 ttagaaaagt tttatggaaa atcaagtaag gttaataata tgtattttgc aaaagatgga
    21301 agtggtagag aagctgattt agttgatggg cttgctgctg gagtattagc atcaaaaaca
    21361 aagtcacctg tagtacttgc aagtggaagt ttaagtagtg cacagaaaag ttttataaaa
    21421 aaagtaaagg ctgataaatt tgtacaagtt ggtggaggta aaaatagtaa accatatgca
    21481 gaagcattag ccttacagta atattgtgta aatcaaaatt aatatataat aatagcaagt
    21541 aaaccaagtt gcacttcaat atatataata aaataagtaa actaacctat gtttaaatat
    21601 aatatataat aaaataagta aactaagtta tacttcagta taatatataa tgaaataagt
    21661 aaactaagtt atactccaat ataatatata ataaaaacaa gtaaactaag ttatatttaa
    21721 atatagcata taataaaaca agtaaaccag attctattta attagaagtt taaggtattg
    21781 tacttaaact tctaattgag atagaaataa aatggtttac ttgtttttat tatgaattta
    21841 ataaattaaa gcttatttta tttttattaa aaacttacta atcattgata tttattatat
    21901 ttccagtatt gatgtcaatt aagtttatat caagttcaag ctcatcctca tcatcagttt
    21961 ttatgatatc tataattgcg acagaatggc ttccatcttt tgggatagaa gtacttcctg
    22021 gatttattac tattaaatta tcatccatgt atagtttttt tacatgagta tgtcctaaaa
    22081 ctaatatgtc accacccatt ttctttgctt tgtcaataat ttcttcttct gattctatat
    22141 aaccgtgatt caatataatt ctaatttcac caaattggct catgacataa ggactctgaa
    22201 taggatgttc tataaccatc tggtcaacat ctgcatcaca gttccctttt acaatcacaa
    22261 tgttttctaa tttatttaat gtatcaataa attttttagg attatatcct tctggaatat
    22321 catttctagg tccatgatat aagacatctc caccatgtaa taaaacatca caatcagata
    22381 atacatttaa cgctttttca aagtaaagta agctaccatg agtatcactc attattccta
    22441 ttttcatatt aaaaatcctc ctaagtacag tctataaaaa tttttcagat actctatgcc
    22501 aaaactcata gtttgacatt ctaagtaatt ttatttttgc atcagatata ctaactttta
    22561 tattttcgat ttgtctaaat ctgtgctcaa ctccatcaac gacaatcaat acagaatctt
    22621 caaatctata ttctggtgca atctttataa cagattcatt tgaacatatt atacttgatg
    22681 taaagcacct atatgcattg gtatttatcg gatgtaaagg tgtaatctgc ataagttcaa
    22741 gatttatatc aacaatacta cctccagcag aatagttata ggctgttgaa cctgtggaag
    22801 tagaaataat cataccatct ccactaaaat tttgtatatg tttattatca aggcttaaat
    22861 ttaggtgtat agtcctagac ttgtctcctc ttataacaac ctcatttaca gcaagcatat
    22921 tagaaccact agtagtagta tatacttccg cattaagaag tgacatttct tgaataatat
    22981 aatctttttt tgtataagct tcaataaagc tatcaatctt atctggtaaa atatcaggaa
    23041 aaaaccccag atgacctgta tttattccca ttatgggaat ctcaggaaaa tcgaaatctc
    23101 taactgttct taaaaaagag ccatcaccac caattgatat aataagctct gtgtctggat
    23161 aaatatctga atacacttca aagccagcat tgataagttt tctagttaag agttctttag
    23221 tctcaaggga cttatttaat tggtttgtat ttatagttat aattcttttc atatattcac
    23281 ctcttcatac tttgtattat aacatagttt tataattctt aatttataaa tttataaatg
    23341 acctttgtag tgaatgtgtt aaaatatgta taggtgggta taaaaaaatg cataagaggt
    23401 gaagatgtgt ttagcataag gaatatcaca aaaaaattag gaaaattcaa gttaaataat
    23461 ataaacttag agttaaagga aggggatatt gttgggatta taggaccaaa tggttcggga
    23521 aaaacgactt taataaaaat tataatgggg attattgatg cagatgaagg tgaaattgaa
    23581 ctttgtaatg aaactattga aaattctcca ataagcttta aaaacaatat tggttttgta
    23641 tatgattctt tacaatttta tccgcattta aaggttaagg aatttagaaa aatagtttct
    23701 ttattttata agaattttga tagagaaagg tttgatgaat accttaataa gtttgatata
    23761 gaagaaaata tgcatattga aaatttatct aaaggtcaat ctgagaagct tatgttatca
    23821 agtgcactta gtcacaatgc taagttattg attttggatg aaccaactgc tgggatagac
    23881 ccaattgtaa gaactgaaat aatgcagtat ttacaagatt ttgtgaaaaa tggaagtagt
    23941 agtgtaataa tttctactca taatactgat aatttaatta aaattgccga ttatcttgtt
    24001 tttataaata gaggaaatca gatttttact gttaagaaag aattaataga acaagaatat
    24061 aagataatta gggcaaataa agcagagtta gaagcaatta aagaaagcat agttggtgtg
    24121 aaagagtata aatattataa tgaggcactt gttaaagttg gagatagctt gaaagtaaaa
    24181 agcctattga tagaaattga taagcataag gtcaaaaatc ctactataga agaactaatg
    24241 tattattatg taaatgaggt gagatgatgt tcaatttaat aagaaaagat ttaataatag
    24301 ggattagttc agatggtatc cgtaatttaa agtacatatt gctatttttt gtgttttatt
    24361 ttttccttaa ttcaatttca tattatacag taagtattgt tgttagttat ttgatattta
    24421 taaatacatt tgagtgtgat tatgaaaatg actcaaggat ttttataagg agtatgccag
    24481 ttagtataga agatatagtt tactcaaaat atttattagg tgtaggatta ataatttctg
    24541 taacaataat tgtgtcctta ctaagtaaat tgacatcact agtgttcttt agaaatatgg
    24601 ttttaaatga tgtatttttt tctgtaaata tatttttagc aattttgacg attttactgc
    24661 ctttaatttt taagtttgga tatggaaaaa tgaaagtttg tggttttata gtaagtattt
    24721 taatttactt tgtatatgga agttttttga agatgattag tatgattgta tatcaagtaa
    24781 agcattttaa ttatagtaaa gttggaggag tatatctttc aaattatgta actgatatag
    24841 ctaatactaa gtatataaat ttatatagta tgacattttt aacaataata atttttataa
    24901 tatcaatgta tttttctatt aagatttcaa aaaagaataa gtttaattat taaaatttta
    24961 attaatatta tagcacaata gcacaaaata taaggagact aaaaatgaag aagtttataa
    25021 aagtttcatt ttttattttg gggattttta cactattcat agctataaat aaattagtat
    25081 atagagatat ggtaagtgtc aaagattatg aaataaatca tttagaggaa attgtttttg
    25141 attttgatag caggagaagt agagatatcg gtactattgt aagatttaaa ataagcaata
    25201 atagtaaata tagttataaa ttaaatagtg ctaaaatgaa gtttgaaaat tgtgtggaaa
    25261 ataaaaaggg aatgaataag tgttctgcat atgtaaatgt agatttattt aatgaaaaaa
    25321 attctataac tgaaaggact ctgaaatatg gaataaaacc aaataatcaa ggttatgttg
    25381 attttgtaat tccaaaagga ctaaattttg attataggta ctttaatgaa tctggtatga
    25441 gtgtagaata taaaggagaa tatattgtca atattccaat ggcaaaagga ctttatctag
    25501 tagttggaaa aaataatgat acatggaatg ctaatattat ggaataattt gaaaaataaa
    25561 tcaattatag atataattaa gaataatatg aaatttataa aagttcagac taggaggggt
    25621 atttaatgtc aataagtaac gtaagagagt attttaaaca atttggcaag gaagacagta
    25681 tattagaatt tgaacaatct agtgctactg tggaacttgc agctgaagca gctggggtta
    25741 ttccagcaag aatagctaag acactctcat ttaaaatagg agatgatgca attttaattg
    25801 tgacagctgg tgatgctaag attgataata agaagtataa ggctgaattt aattgtaagg
    25861 ctaaaatgtt gacaccagaa gaagtcttgg agtttactgg acatgctatt ggtggagtct
    25921 gtccatttgg tttaaaaaat tctataaaag tctatttaga tgattcaatg aaaagatttg
    25981 atacagtatt tcctgcatgt ggcagtagta actctgcaat agaacttaca tgtgaagaaa
    26041 tggagaagtt ttctaagtgt gaaaaatggg tagatgtttg taagaattgg taaaaaaata
    26101 tagtaaacta tagtagaaat tagtaaaaaa ctacctctgt atacaatcta tacagaggta
    26161 gtttttttgt gtaaatattt ataaatctaa gaggaaaatc taatttaaac taataatcca
    26221 aaataaagtt aaaaaattct gatttttttt cgaaacaata aaaaaatgac aaaaaagtgg
    26281 aaaaaatgta ataaatattg ttaaaaaaat aattagaaat tattgaaaaa ttatcctttg
    26341 agtgatacta ttatagatgt aagatattta aaaataaaaa atagtcagat aaaaatacgc
    26401 ttatttagct ctattaaaat aatttttata cgataaaaat cttctgtaca tggctaaaaa
    26461 acagatgtac agacaatatt taatgtttaa tataccctaa tataaatatc gtaaacaaat
    26521 tattttatct gtaaccagtg tgaaagaaat cttattctaa cttgaaattt cacaaaattc
    26581 caaaaagcga gattttttta agtatagttg attttaaatt aacaatttat gattaaatta
    26641 atgtcataat tggttagtat aatcacatat aaatatagag cgtagtttga ttaaggttat
    26701 aaatttctcg acgtatattc aaatacctta ttatagatgt tgccgagatt ttaattaata
    26761 aatgaaaata aaatatttat aaaatactga ggaggctatt atggatttaa attctaaaaa
    26821 atatcagatg cttaaagagc tatatgtaag cttcgctgaa aatgaagtta aacctttagc
    26881 aacagaactt gatgaagaag aaagatttcc ttatgaaaca gtggaaaaga tggcaaaagc
    26941 aggaatgatg ggtataccgt atccaaaaga atatggtgga gaaggtggag acactgtagg
    27001 atatataatg gcagttgaag aattgtctag agtttgtggt actacaggag ttatattatc
    27061 agctcataca tctcttggct catggcctat atatcaatat ggtaatgaag aacaaaaaca
    27121 aaaattctta agaccactag caagtggaga aaaattagga gcatttggtc ttactgagcc
    27181 taatgctggt acagatgcgt ctggccaaca aacaactgct gttttagacg gggatgaata
    27241 catacttaat ggctcaaaaa tatttataac aaacgcaata gctggtgaca tatatgtagt
    27301 aatggcaatg actgataaat ctaaggggaa caaaggaata tcagcattta tagttgaaaa
    27361 aggaactcct gggtttagct ttggagttaa agaaaagaaa atgggtataa gaggttcagc
    27421 tacgagtgaa ttaatatttg aggattgcag aatacctaaa gaaaatttac ttggaaaaga
    27481 aggtcaagga tttaagatag caatgtctac tcttgatggt ggtagaattg gtatagctgc
    27541 acaagcttta ggtttagcac aaggtgctct tgatgaaact gttaaatatg taaaagaaag
    27601 agtacaattt ggtagaccat tatcaaaatt ccaaaataca caattccaat tagctgatat
    27661 ggaagttaag gtacaagcgg ctagacacct tgtatatcaa gcagctataa ataaagactt
    27721 aggaaaacct tatggagtag aagcagcaat ggcaaaatta tttgcagctg aaacagctat
    27781 ggaagttact acaaaagctg tacaacttca tggaggatat ggatacactc gtgactatcc
    27841 agtagaaaga atgatgagag atgctaagat aactgaaata tatgaaggaa ctagtgaagt
    27901 tcaaagaatg gttatttcag gaaaattatt aaaatagata aatattaaaa tatacacaat
    27961 aggtcgatat taaaatagat atggaggaag gatttatgaa tatagtcgtt tgtataaaac
    28021 aagttccaga tacaacagaa gttaaactag atcctaatac aggtacttta attagagatg
    28081 gagtaccaag tataataaac cctgatgata aagcaggttt agaagaagct ataaaattaa
    28141 aagaagaaat gggtgctcat gtaactgtta taacaatggg acctcctcaa gcagatatgg
    28201 ctttaaaaga agctttagca atgggtgcag atagaggtat attattaaca gatagagcat
    28261 ttgcaggtgc tgatacttgg gcaacttcat cagcattagc aggagcatta aaaaatatag
    28321 attttgatat tataatagct ggaagacagg cgatagatgg agatactgca caagttggac
    28381 ctcaaatagc tgaacattta aatcttccat caataacata tgctgaagaa ataaaaactg
    28441 aaggtgaata tgtattagta aaaagacaat ttgaagattg ttgccatgac ttaaaagtta
    28501 aaatgccatg ccttataaca actcttaaag atatgaacac accaagatac atgaaagttg
    28561 gaagaatata tgatgctttc gaaaatgatg tagtagaaac atggactgta aaagatatag
    28621 aagttgaccc ttctaattta ggtcttaaag gttctccaac tagtgtattt aaatcattta
    28681 caaaatcagt taaaccagct ggtacaatat acaatgaaga tgcgaaaaca tcagctggaa
    28741 ttatcataga taaattaaaa gagaagtata tcatataatt taagggggga taaagaatgg
    28801 gtaacgtttt agtagtaata gaacaaagag aaaatgtaat tcaaactgtt tctttagaat
    28861 tactaggaaa ggctacagaa atagcaaaag attatgatac aaaagtttct gcattacttt
    28921 taggtagtaa ggtagaaggt ttaatagata cattagcaca ctatggtgca gatgaggtaa
    28981 tagtagtaga tgatgaagct ttagcagtgt atacaactga accatataca aaagcagctt
    29041 atgaagcaat aaaagcagct gaccctatag ttgtattatt tggtgcaact tcaataggta
    29101 gagatttagc gcctagagtt tctgctagaa tacatacagg tcttactgct gactgtacag
    29161 gtcttgcagt agctgaagat acaaaattat tattaatgac aagacctgca tttggtggaa
    29221 atataatggc aacaatagtt tgtaaagatt tcagacctca aatgtctaca gttagaccag
    29281 gggttatgaa gaaaaatgaa cctgatgaaa ctaaagaagc tgtaattaac cgtttcaagg
    29341 tagaatttaa tgatgctgat aaattagttc aagttgtaca agtaataaaa gaagctaaaa
    29401 aacaagttaa aatagaagat gctaagatat tagtttctgc tggacgtgga atgggtggaa
    29461 aagaaaactt agacatactt tatgaattag ctgaaattat aggtggagaa gtttctggtt
    29521 ctcgtgccac tatagatgca ggttggttag ataaagcaag acaagttggt caaactggta
    29581 aaactgtaag accagacctt tatatagcat gtggtatatc tggagcaata caacatatag
    29641 ctggtatgga agatgctgag tttatagttg ctataaataa aaatccagaa gctccaatat
    29701 ttaaatatgc tgatgttggt atagttggag atgttcataa agtgcttcca gaacttatca
    29761 gtcagttaag tgttgcaaaa gaaaaaggtg aagttttagc taactaattt cttggggagg
    29821 gtattatgag tacaagtgat gttaaagttt atgagaatgt agctgttgaa gtagatggaa
    29881 atatatgtac agtgaaaatg aatagaccta aagcccttaa tgcaataaat tcaaagactt
    29941 tagaagaact ttatgaagta tttgtagata ttaataatga tgaaactatt gatgttgtaa
    30001 tattgacagg ggaaggaaag gcatttgtag ctggagcaga tattgcatac atgaaagatt
    30061 tagatgctgt agctgctaaa gattttagta tcttaggagc aaaagctttt ggagaaatag
    30121 aaaatagtaa aaaagtagtg atagctgctg taaacggatt tgctttaggt ggaggatgtg
    30181 aacttgcaat ggcatgtgat ataagaattg catctgctaa agctaaattt ggtcagccag
    30241 aagtaactct tggaataact ccaggatatg gaggaactca aagacttaca agattggttg
    30301 gaatggcaaa agcaaaagaa ttaatcttta caggtcaagt tataaaagct gatgaagctg
    30361 aaaaaatagg gctagtaaat agagtcgttg agccagacat tttaatagaa gaagttgaga
    30421 aattagctaa gataatagct aaaaatgctc agcttgcagt tagatactct aaagaagcaa
    30481 tacaacttgg tgctcaaact gatataaata ctggaataga tatagaatct aatttatttg
    30541 gtctttgttt ttcaactaaa gaccaaaaag aaggaatgtc agctttcgtt gaaaagagag
    30601 aagctaactt tataaaaggg taaaaatcta attaaaaata tatttgctgt taactagatt
    30661 aaatagtatt ttaatctagt taacagaata taaatactaa aataacagga ggttataact
    30721 atgaaattag ctgtaatagg tagtggaact atgggaagtg gtattgtaca aacttttgca
    30781 agttgtggac atgatgtatg tttaaagagt agaactcaag gtgctataga taaatgttta
    30841 gctttattag ataaaaattt aactaagtta gttactaagg gaaaaatgga tgaagctaca
    30901 aaagcagaaa tattaagtca tgttagttca actactaact atgaagattt aaaagatatg
    30961 gatttaataa tagaagcatc tgtagaagac atgaatataa agaaagatgt tttcaagtta
    31021 ctagatgaat tatgtaaaga agatactatc ttggcaacaa atacttcatc attatctata
    31081 acagaaatag cttcttctac taagagacca gataaagtta taggaatgca tttctttaat
    31141 ccagttccta tgatgaaatt agttgaagtt ataagtggtc agttaacatc aaaagttact
    31201 tttgatacag tatttgaatt atctaagagt atcaataaag taccagtaga tgtatctgaa
    31261 tctcctggat ttgtagtaaa tagaatactt atacctatga taaatgaagc tgttggtata
    31321 tatgcagatg gtgttgcaag taaagaagaa atagatgaag ctatgaaatt aggagcaaac
    31381 catccaatgg gaccactagc attaggtgat ttaatcggat tagatgttgt tttagctata
    31441 atgaacgttt tatatactga gtttggagat actaaatata gacctcatcc acttttagct
    31501 aaaatggtta gagctaatca attaggaaga aaaactaaga taggattcta tgattataat
    31561 aaataagaat ttgggaatta aaagtttaaa taaaattgtt taaaaaacaa tttcggatat
    31621 atgaaaaatc taatttaatg ggggtaatga atatgagaga agtagtaatt gccagtgcag
    31681 ctagaacagc agtaggaagt tttggaggag catttaaatc agtttcagcg gtagagttag
    31741 gggtaacagc agctaaagaa gctataaaaa gagctaacat aactccagat atgatagatg
    31801 aatctctttt agggggagta cttacagcag gtcttggaca aaatatagca agacaaatag
    31861 cattaggagc aggaatacca gtagaaaaac cagctatgac tataaatata gtttgtggtt
    31921 ctggattaag atctgtttca atggcatctc aacttatagc attaggtgat gctgatataa
    31981 tgttagttgg tggagctgaa aacatgagta tgtctcctta tttagtacca agtgcgagat
    32041 atggtgcaag aatgggtgat gctgcttttg ttgattcaat gataaaagat ggattatcag
    32101 acatatttaa taactatcac atgggtatta ctgctgaaaa catagcagag caatggaata
    32161 taactagaga agaacaagat gaattagctc ttgcaagtca aaataaagct gaaaaagctc
    32221 aagctgaagg aaaatttgat gaagaaatag ttcctgttgt tataaaagga agaaaaggtg
    32281 acactgtagt agataaagat gaatatatta agcctggcac tacaatggag aaacttgcta
    32341 agttaagacc tgcatttaaa aaagatggaa cagttactgc tggtaatgca tcaggaataa
    32401 atgatggtgc tgctatgcta gtagtaatgg ctaaagaaaa agctgaagaa ctaggaatag
    32461 agcctcttgc aactatagtt tcttatggaa cagctggtgt tgaccctaaa ataatgggat
    32521 atggaccagt tccagcaact aaaaaagctt tagaagctgc taatatgact attgaagata
    32581 tagatttagt tgaagctaat gaggcatttg ctgcccaatc tgtagctgta ataagagact
    32641 taaatataga tatgaataaa gttaatgtta atggtggagc aatagctata ggacatccaa
    32701 taggatgctc aggagcaaga atacttacta cacttttata tgaaatgaag agaagagatg
    32761 ctaaaactgg tcttgctaca ctttgtatag gtggaggaat gggaactact ttaatagtta
    32821 agagatagtt ttagattata atatttacaa attaaatatt taattatgaa tttaacctgt
    32881 aagaataact atttgttatt cttacaggtt tttatagtta aaaataatta ttatctatta
    32941 aacaaaaata gtgtaaaata tataatatca tgattttgaa gatataaaaa gtaggaagat
    33001 ataaaagaaa gtaggtaaaa gtttgtttaa aaaagagaat caaagatata atcttatatc
    33061 gtatacaaat gaagaagaaa tgactttaaa agaagtttta ttggacaagc taaatttctc
    33121 agttaggtca ttatcaaaaa tgaagaggga aaagagtgtt ttagtaaatg gagtatataa
    33181 aaaaccgagt ttaaaagtgt atagtggtga tttaatagaa gtaaaaatag acgaggaaaa
    33241 agcaaatttt gaacctcaag atttaaattt acagataatt tatgatgatt ttgatataat
    33301 tatggtgaat aaacctccgt ttatggtagt tcacccaaca aaaagccact atgataaaac
    33361 aatagcaaat ggtattagtt actatataga taatcaaaaa gaaaatgtaa aaataagatt
    33421 tgtaaatagg ttagatatga atacttcagg gttagttata gtagcaaaaa atgcttatgc
    33481 acatcatact ttatctacag caatgagtga aaataaagtt gaaaaaaagt atataacagt
    33541 agttgatgga attataaaag aaaatgaggg aactatagat gagccaattt atagacctac
    33601 agaagattca ataaagagga taatagatga aagaggacag tcatctgtta ctcactataa
    33661 agtaatagaa agactagaaa atgcaactgt attagaagta agcttagaaa ctggtagaac
    33721 acatcaaata agagtacata tggctcatat aggtcatgga ataataggtg atgagttgta
    33781 tggttatgta gatgaagagc taataaatag gcaagcactt catgcataca agctagagtt
    33841 tgaacaacca agaacaaaag aaaaattgaa atttaaagca gatataccag aggatatgaa
    33901 agagttaata tcgaaattaa gataatgggg tgatatatat gataatacat aaatttataa
    33961 tacatgtttt ggacaaaaat agtgatgttc caatattaaa tgactttgaa ggcaaagtca
    34021 atcaagaagt ggatggtttt ttccagaaag ctataaaaag aattgcaaaa gatgaggatt
    34081 taagaaaagg tgtttttaaa gattataatg ataatttaat taaaaactgt tgtgaacaaa
    34141 taatatatga tgaaagtact tttttgaaaa attctaaaga aattgcatct tacttgtttg
    34201 acgttatgaa aattaatgct gcactagaat cttgtgattt ggcaatttgc ttatacacta
    34261 ttaaagatga aaagagtgta gcaatactga aattagatta taaaaagttg tacactcatt
    34321 caattgaatt tgtggatgat aaatttaata ttcaaatggt ttcaaatgaa attggtatcc
    34381 cagagacttt aagacaaaag caaggagctt tgattagtct aagtggtata aatgatgagt
    34441 ttcatctaag gcttttagat aaggatgctg aaaaagaagg gtcagaatct aagtttgtaa
    34501 cagaattttt aaatgctaaa aaaatagatg atgataagta caagaccaag gtatttaaaa
    34561 atacagctga aaattggata accaatgctc ttagtaatga tataaagcaa gctgaagatg
    34621 taagaagtat attaaattat acactaaagg aaaaacatga agttgatata aatgactttg
    34681 ttgataattc aattaaagat gatgagttaa aggatagctt taaagagcat atggaagaaa
    34741 aaggtcttga tgaaagtttt agtatagata aaaaatgggt tgagaaaaag cttaaaaaaa
    34801 gaagtataaa aacggataat ggttttgaca taaaagggaa tctaactgat tttgaagacc
    34861 caatgaagta tactgtaaaa caaaatcaag atggaactat agatataata ataaagaatg
    34921 ttacgtttta tgaagaaaag taatttatat acttagtata taaattaaat ataagagcgt
    34981 gtattttata tattgtaaat aaatgtaatc tagtctgtgc gaactctaat ataatttatt
    35041 atgtagaagt ttacacagac tatttatatt tgtagacaaa acgatactat ttgttttctt
    35101 cgatatattt acatatatca ccaatacatt taaagttctc agcttcctcg tctggtattt
    35161 ctattccaaa ttcatcttca agggccatta taatttcaac agcatctaat gaatcagctt
    35221 ctaagtcgtc cattaaagat gcttccatag ttatctcatc taaattgtct acccctaatt
    35281 gttctgctat tatttctttt attttctcaa acattttaag cctcctaaag cagttttaat
    35341 taattttaat gttagtttaa tataatatca agaatttttc aatatggaat ctataaaaaa
    35401 taataaaatt ttataaatgt agtaataaag tattatttga tgaataaact ttattgataa
    35461 caaatataac ttaggaggaa atgctatgaa atatgtagga ctattactaa cttcagtagg
    35521 aatgttttta cttatagcag taaattttta ttataattca gtaactttag atatgcaacg
    35581 tatagaggat tatgtaatgg agaccaattt aatccttgaa gatgtagctg aaaaggagag
    35641 ttatgtatct aatgaaaaag aagattacat atcaaggtta atgcatgtaa agaaaggaat
    35701 agaaaattca aaaacatcct tcttgataga aagatataag gagtacaaaa taaaatcaat
    35761 agaaagttta atctatacta tatcagagga gaaaaaagat tatctggatg aagttgatag
    35821 atacaataaa cttggtgaaa aagaaataaa taaactaatc aataaaaact ttttagaagt
    35881 aacttatctg tctattacta catatattta attaaagaga caaaacttag tctcttaatg
    35941 attcaaggag gattagttat gacaaaatta gaagacaata tggaacgttt ctttggagga
    36001 tgcgaaggat tttttgggaa taaaatgttg ataataatag ttatagtatt cttactatta
    36061 tgcacagaca ttttagaaga cttattatgc gatgacaata tatggatatg ggttatatta
    36121 atagtattgt tattatttaa cttcgatgat ggttgttgtt gctaaaaaat attgattatt
    36181 aaataggtaa aaccagtaac ttatacaagt tatggtttta ccttttttag ttcaaattaa
    36241 aaaatttaaa aaaatagtaa aaatacatta gcaaaatgga aaccaaatgc atagtatatg
    36301 ttaggagcga aaagctcaac aaaatatcaa caggaggtaa cgtataatgt tagatagatt
    36361 ctttggagaa gaaggatgtt ttggtggagg aggatggtgg ataatagtac tattcttcct
    36421 attcttagct tttggagata gctgggcaga atttgacata atggcatgga taccattcct
    36481 aatagtttta ttaatacttt gctgctgtgg tggatgctta gaataagaac acagttatta
    36541 aaaaaaggag ggctttgtta tgttagatag attttttgga gaagaaggat gctttggtgg
    36601 aggaggatgg tggataatag tactattctt cctattctta gcttttgaag attgctggag
    36661 agacattgac ataatgtcat ggataccttt cttaatatta ttgctaataa cttgttcatg
    36721 tggtggattc tttgatggtg aagtaggttg cggctgctaa tacttaaatc attagaataa
    36781 aaaaatgctg gtattttata ccagcatttt tttattttat tttttaatat gatttaaatt
    36841 aagaatgaat attattaaat ttgttgaata tagggtataa tataaaatat aatgtattta
    36901 aatgtacttg cttttatggt aaaataaaaa cttatataaa tgtagttaaa ataggaacct
    36961 tcaaaaattt ggttcagttt tagataaatt tagtaaaata aggaggatga agatgaaagg
    37021 caatataacg ataaaagatg ttgctaaaca agcaggagtg tcaatatcta ctgtatctag
    37081 agttataaat gattcaaaac ctgtaactga tgaagtcaaa caaaaagttt tagaggttat
    37141 aaaagagact ggatatatac caaatccact tgctagaagc ttagtaacaa agaaaagtca
    37201 attaataggg gtaatagttc cagaagtttc agattctttt gttaatgagg tgttaaatgg
    37261 gatagaagag gttgctaaaa tgtatgacta tgatattctt ttagcgaata catactctga
    37321 taaggaacaa gaacttaaga gtataaatct attgagagca aaacaagtgg aaggtatagt
    37381 tatgatttca tggatagttg aacaagaaca tatcaactat atacaaaatt gtggaatacc
    37441 agcgacatat ataagtaaaa ctgctagaaa ttatgatata tatacagtaa gtactagcaa
    37501 cgaagaagct acttttgata tgacagagca tcttataaag aaaggtcatg aaaagatagc
    37561 ttttataatg acgagtaaag atgatactgt tttagaaatg gaaagacttg ctggttatga
    37621 gaaagcactt tcaaataaca atatagaatt agacaagagt ttgattaagt atggtggaac
    37681 tgattatgag agtggataca atagtatgaa agaactatta gatgatggaa taatacctca
    37741 tgcggctttt gtaacaggtg atgaggctgc cataggtgct ataaatgcta tatgtgatgc
    37801 tggatataag gttccagaag acatatcagt tgcaggattt aatgatgtta agatagctag
    37861 aatgtataga cctaaactta ctacagtata tcaacctcta tacgatatgg gagcagtagc
    37921 aataagaatg gttataaaat taataaataa ggaattaatt gaaaataaga aaatagaatt
    37981 accttataga attgttgata gagaaagtgt cacagaaaga aaaaaataaa actattaaag
    38041 tcaatcaggt agtgcctaag gcactacctg attttttatg tcaataatat caaatttaat
    38101 aatagattaa ctaaaaataa ttatctaatt acttaaatag tttattttta tctattcatg
    38161 agggttagac tttttagttt tcttaaataa acttttagat tttttttgtt gactacctgg
    38221 tgtatttttt ggtgcaggtg aatttgactt acctttagta ggtttagcct tcttagtttt
    38281 agctggttta gtatctcttt ggttttctgc atctgagatt ttttccatta tattatccag
    38341 ttttttgcgt tgattttcat ctaaaaatgc tttgaatttt gatactactt catctctgcc
    38401 atctaggttc ttacctattt ttataagttc ctccataagt tcattttctg attttccttt
    38461 gtaactatca gccatctttt ctattttatc cttgtcaata tttttttgtt tagccatttt
    38521 ttctaatgac tctgtatcta attttttcat aaaaaaaccc ctttctataa atatatattg
    38581 aattatattc atactattta tatatagaaa taatattgaa gtggtgaatt gtgagtgata
    38641 tataatataa ataaaaattc ttatagataa tatatatgca ctgagcttta aaaatggaac
    38701 attaaaatat gaaaaacata ctttaatata gggcaattat ataatataag ggggaattgt
    38761 cttgaataac ttactatttt tgattggagt gattgcattg agtaatggta atatctctat
    38821 atttgatgat aaaaataaat caaaaggaaa taatagagtt agaaaaaaca aaaaaaatgc
    38881 aaatgataaa ctttcacagt ctacacatgg caatttaagg tttgatgcaa atgatataaa
    38941 aagaggttta aggctcatgg atttaagtga agaagattta gaaatgggaa tggaaataat
    39001 aacaagaact aaaaaataca tgtcaagaga tgaaagaaaa atactaataa aaatagaaag
    39061 tgttttagat ttagttagag gaataaaaaa attaaataat atagatatgg tagatacaga
    39121 agatgaaacg gattttttca gaaaaatgga tagtgatgat aaaaagaata tgatgataaa
    39181 ggaaattata gatgtattcc cagaaaaaag aaaaaattca gttgaaaaag ctataggaat
    39241 gaaaaagaag atagatttat ttgcagaatt atttttacca gatgattttg gtgaaggtgg
    39301 atttagtttg agctctttgg caaatataaa caatcttggt agtatgaata atctcaaatt
    39361 gttaggaagt cttttaagag gtgatgatag taataatgat gatgaagaca ataatgatga
    39421 gttaaattat gaagacaaag aattggatta tgatgatgag ttaacagatg aaggtgaaat
    39481 ggaggataat gaagatgaat tagtagaaga taagaacaca ggctatgaaa atatagaaaa
    39541 tgatgaagat atagtatacg atgaggaact atacaacatt aatgagcaag aatccgaata
    39601 caattataat gaaaaaaata ctaaaactag agctaagaaa aaacgataat taactaaata
    39661 cataaagaaa aaaggaagaa aaagtaatga tagaataaga ttatctcaat tactatataa
    39721 aaccacttta aaaaattcaa aatttattat ataataataa tatgaaatcc tgaaacatac
    39781 gataaggctt ttttaaattc aacctacaaa tgaaatacgg gagaccgtgt ctggtaaatt
    39841 ataacatgcc tagttagttt ggacgttaaa ggttactggc aagtcaccca cctgggagtg
    39901 cctagggtgt gaatttactt agccaccggt gtttcgggac aaaaaaagtg ctactatttc
    39961 aaatagtagt acttttttta ttaatatgca tttttctact aaatacgcct aattattgat
    40021 aaattcaaaa agcaagtaac aattaagatt ataaaagcat agtattaatt aggcttcaaa
    40081 aagccaaaga cttaataaga atcgcttaca gggagggcaa tgaaaaatgc aagattataa
    40141 aaaaaataaa agaagaatga tgaatcagcc aatgtctaca atgaatgaag aagaagtgta
    40201 tacagatgaa ataaattcag aagacatgag aggttttaaa aaatcacacc atcataatgg
    40261 atgtaatact gataataagt gtgagtgcca tgatgattgc aatccatgca acccatgtaa
    40321 tccatgtaaa cctaacccat gcaatccatg caaacctaat ccatgtgatg acaattgtgg
    40381 atgccatgac aattgtaaat gtgattgtga accatgtgaa atggattcag atgaatgttt
    40441 tgaaaacaaa tgtggaccag aatgctgtaa tcctatatct ccaagaaact tctctgtatc
    40501 aaatgcagtg ccatttgcaa tagaggctaa tagaatattt gatactatgc aattccaaac
    40561 atttacagat gcaacaggac caaatggaga gccattaact tttgaaacag aagtagtaga
    40621 agtatttggt tcagttccaa gtgcaggtca agcaagtgta actatagaaa aaatatgctt
    40681 aagtaatgat ggaatagtta tagacacagg aatgacaact ttagaagatt tcgatttaga
    40741 cccattagga gatatagtag gaagaaactg tgaaacaact tttgaatttg cagtttgtgg
    40801 agaaagaaac gctgagtgct gtagacaagg aaaaggcaaa tcagtagctt ataaacaaag
    40861 aggattaact gtagcagttc gtaatttagt actagagcta agaggtagat gtggatgtac
    40921 agagttcgtt gcattagctt tcccagcagt tagagcagga ggtggatgta agagaagagt
    40981 tgattatgta gaatttactt ttaacacact ttcagcacca atatgcttgc cagctgacgg
    41041 aagagctgtt actttaagac aagaatatca aactaactta actgtagatt gtataggaaa
    41101 atctatatta aaattagaat gcaacgaatg ttgtgaacct ttctatgaat taattatacc
    41161 aaatgatata gatttagtac tttgcttaca agaaacagtt agcacattaa taagtgaaca
    41221 aatagtagtt ttagcatcac caaatccaat ccaaccaaga cttgttgata ctttctctaa
    41281 agtatgtgat ttttcgcaat gtggacctaa tcatggaagt ggaaagccaa gttgccacag
    41341 atagataggt aagatatata gacagtttaa attataaata aaaatcatct aaggaaggct
    41401 accaaagtgt ataatttaca ttggtagcct tcacgttttt ataactagcg tatagatttt
    41461 agtgatttaa aaagcactat agtatgtaat agacaagtga agattgatac tatcgtcatt
    41521 gtgtatatgt atgcataaat attaagtcct ggaattggaa gaaatacata taaagctatt
    41581 agttgaatta acatgccagt aatagtattt atacttgaag ctaattcttt tcttattgaa
    41641 tgtaaaatgc cagatagagt ttgatttagg gacataaata gtggaactaa aaacatagct
    41701 tttagatatt ctcctgcaag tgtattttta aatactaata tacataaatc ttttccaaag
    41761 aaataaaaaa atattgatga tagtatgcct acaaataaag taagaagtat ggagtatttg
    41821 atttttttaa ttacactctt acgctttctt agagccattt cctgtgaaat agttggtatt
    41881 aaatttacaa taagcgctga accaacagta aaaggtaaaa atataaaagg cattaccatc
    41941 ccactgacaa taccatacat acttaaagat tgttgataag tcattcctga taatgctaat
    42001 cttgatggta tcatcataga acttatagat tgcaaaagta tgtttgacat tctattacaa
    42061 gttatgggaa tagacatttt taatgtttcc atagaagaat tataaaaatc ttttatgctt
    42121 ataatatacc tattgtcaaa tgatgaatct cgccataagc aaataagcat aaatataata
    42181 ttactcaact caccaatcga tatacctaat agagctatat aacaattcat agatttatta
    42241 tttatgtaca taacaagtaa aaaaacaaat aatattttac caatttgttc aatgatttgt
    42301 ccaatagcag ggatttttac attctttatg ccatagtaat atcctcttag tacgtttgaa
    42361 agagttatta taactatagc aggacaaaca gctagtataa atagatttaa tttagcatct
    42421 tttaaaaatt taagtgataa aaaagaacta ttgaaagatg caactataga tataattagt
    42481 gctacgaaaa atgatatata taaagttgat ataaataaag cattagtatt gtgtctatcg
    42541 tttaaagctt ttctctttgc aactagacaa ctaagtgcag taggtatacc tgttgttgta
    42601 acagctaagc aaatcatcaa aaagttaaaa atcatatgat agatacctaa acctgtttca
    42661 cctagtgctc ttgataagaa tattttatat aaaaaaccaa atattcttac aataaaattg
    42721 gatataaata aaataactgt tgatagtact aaataaggta ctttcaaaaa aatcacctcc
    42781 ttatctttta aatatattca caaggaggtg taaaaattta taattttata aattctggtt
    42841 tcaatttatt aaatttagat acatatttaa atcccatatt ttttagtaat gaatatatgt
    42901 agtcaaactc acatgcaact tgacttatat gatgtgaatc tgaacctgtg gttatgattt
    42961 cccctcccaa gtctttatac atttgaagta tatacttaga tggggttagg tctggaagat
    43021 tatatctata acaagaggta tttatctcga tacctttgcc atcataaata gcttgtttaa
    43081 gagtttcttc aattatatca gaaaatagtc tatcatctaa aatagtgtca taaggtgcat
    43141 accttttaat caaatctaaa tgacctaaga cagagtaatc tttataattc ttaacaatat
    43201 tatataaata aagatagtaa ttttcataaa cttcatgttg agttttatct ttaaaataat
    43261 taccaaggta taagtccatt gtatctatag catggataga acatattata aagtccaatg
    43321 gatattgatg tgcttccttt ttgtatgtat ccattaattg agtttgaact ccaaattcaa
    43381 ttcctttttt tattgatatt ttatctttat atttgctttg aagagtctct aaactcttaa
    43441 aataatcttc gtagacaatt gaaaaagaat catcagcata tacatcatag tcaacatggt
    43501 ctgtgaaaca aatttcctcc aaaccgagtt caatagattt ttttaccatt tcttccattg
    43561 tagattttcc gtctgtagag aaacttgagt gcatgtgata gtcgtaaaac ataaatattt
    43621 ccccttttta taaaaatttt ggtattatat atacatacta aataatatca aatatacata
    43681 aaagcatcaa taaaatataa atacaatgag cggtgataaa tttggaaaaa tacagaatag
    43741 atttaaatga gaaaaaatca actaaatata tacagatatt taatcatata aaagaattga
    43801 taataaataa agagttagta gaacatgaaa aattgcctcc aataagaaaa ctatcaaatt
    43861 ttttacaagt aaataacact actatagtta aggtatacga gcttttagaa aatgaaggat
    43921 atgtatataa aattataggg agtggtactt ttgtatcaag tttaaattct tataaaagcg
    43981 gtatagataa caggaaaaat aagattattg tgcaaagaga agatttaatt cattttgaca
    44041 atgggaatcc atctaatgat atgtttccta tcgattcatt taaaaatgct gtaaatatgg
    44101 cattatctaa agatggtagt tcaatatttg aatatgatga aggtcttggc tctgaagaat
    44161 taagagaaaa aatggtagag tatctaagtg atggaaatat aaaaactact aaggaaaata
    44221 ttcaaataat atcaggtgct caacaaggta tagatatagt ttgtaaagga cttataagtt
    44281 attcagatgt tgtttttatg gaagaacctt cttacaatgg agcaattgaa gtgtttaaaa
    44341 gtagaggtgc aaaaataata tctataccta tgttagatga tggaattgat ataggtattt
    44401 taaaattaaa gttggaaaaa ataaaaccaa gacttatata cattatgcct aattttcaaa
    44461 atccaactgg aatatcttac tcaacatata aaaaaaagaa attgatagaa ttggcagaag
    44521 aacatgattt ttatataata gaagatgatt ttataagtga ttttgttttt gaatcaaagg
    44581 acaatagaac aattagaagt tatgatgata aaaatagagt agtatatata aaaagctttt
    44641 caaaaatact tatgccaggt cttaggatag gaattgtaga aatgccaaat gagttgttaa
    44701 aaaaagtttt atgggctaaa tattcatcag atatatcaac accaggttta attcaaaaat
    44761 caatgtatta ctatatgaat aattttgatt ggaagaatta tttgagtcat atagagaaga
    44821 tatatactga taagtataaa ttggcaaaaa acttgattaa tgacaaattg agtggtaaat
    44881 taaaagttag aaaatcttgt ggaggaataa actttttttt agaattacca agaggatata
    44941 catctcaagc atttacaaat tttatgttaa acaaaggagt gtcaatgctt ccagggactt
    45001 atttttttga caatctagtg gatgatagat tctttaggat aaatatagct cgtccaagca
    45061 tggaagaact agaaaaggga atatctataa taagtgataa tatagatgag tttttttgtg
    45121 aatataaaaa caagttagat ataaaaagca ataaattatt ttattaaata aatagaggtt
    45181 ttttaggagg aaaaaatgtt tgacaagaag aaattagata ggattaatga attagcaaag
    45241 aaaaataaag agggaatcct ttctgctgat gaaatcaaag aaagagaaat tttaaggaaa
    45301 gaatatttag aaaactttag agcccacttt agatcaagac tagatagtgt caaagtagtt
    45361 tcaccagaag aatatgagca atacatgaaa aacaataaaa attagaactt agtaggagga
    45421 aaatatgaac ttaaagtacg aatttaagaa tgcttgggat tttgaaagaa aagaaaatac
    45481 tattgaacaa attatgcaat attctcgcaa ttatatggag tttttaagta agtcaaagac
    45541 tgaaagactt tcagtaaaag aaattataaa attggctaaa gaaaataact atatttcaat
    45601 agatgaagct atggaaaaat gaagcataag ttgtggtgat aaaatttatg ttataaataa
    45661 agagaaggca gtagcacttt ttgtaatagg taaaaattat atagaaaaag gtatgaagat
    45721 tataggaagc catatagatt cgccaagatt agatttaaaa ccaaatccac tgtatcaaga
    45781 gagtaatcta ggttttttta agactcatta ttatggtgga ataaaaaaat accaatggac
    45841 ggctattcca cttgctttac atggtatagt tatattaaat gatggaacaa aagtggacat
    45901 atctattggg gaagaagata gtgacccagt attttgtgta actgatttat tgattcattt
    45961 ggctggagac caaatgcaaa agaaactatc agaaggaatt agtggagaag ctttaaatat
    46021 attaattggg aatatgcctt tagaagatga agaaaaagaa cctataacag caaatatatt
    46081 aaagatttta aatgagaaat acaatatagt tgaagaagat ttattaagtg ctgaaattga
    46141 agtagtccca gctggtaagg ctagagattt agggcttgat aggtctatgg tgttgggata
    46201 tgggcatgat gatagggtat gttcatatgc tgctgtaaaa gctatactag aaactgaaca
    46261 accagaattt acttcagttg cattatgtgt agataaagaa gaaattggtt ctaaagggaa
    46321 tacaggtatg cactcaaagt tttttgaaaa tactgtggct gaactgattg ctttagaagg
    46381 agattattgt gatattaaag taagaagagc tttagcaaat agtaaggtat tatcagctga
    46441 tgtttctgct ggatatgacc caaattttgg agaagcatat gaaaagagaa attcggcata
    46501 tatgggaaat ggagtagttt taactaagta tacaggttca agaggaaaga gtggatgtaa
    46561 tgatgctaat gctgaattta tgtcagaagt aaagagaatt tttaataaag gaaatgtagt
    46621 atggcaaact gctgaacttg gaaaagtaga ccaaggagga ggcggaacta tagcccatat
    46681 attggcaaat caaggtgcag aggttataga ctgtggtgtt ggagttttaa atatgcatgc
    46741 accacatgag attgtctcta aagtagacat atatgaaatg tataagggat ataaggcatt
    46801 ttttaatata aatttataag taaaaatata aatgtaataa gtgaataaag gctgttgtat
    46861 aaacaattta atctgttgta caacagtctt atttttatgt aaaaatagca atttgtcaca
    46921 ctagagaaaa taaaagaaaa tactgtcaaa ttaaaaatta tttaaaaaaa attgacaacg
    46981 atttcatctt gttataatat gtaataaaga acttgttata taatgttata aaaatgttac
    47041 atagggaggt gagcaaaatg gatgaaaaga gacaaaaaaa agaaatggat ttagaagtaa
    47101 attcctttgt tcctttatat caacaactat atgacaacat aaaaaaacaa atagcatctg
    47161 gcatatataa accaggagat aaacttccat ctgaaggaga cttatgtaaa gaatttaata
    47221 taagtcgtat aactgtgaga aatgcactta atgaacttgt aaaagaagat atattatgta
    47281 aaaaacgtgg taaagggact tatgtaacaa taccagagag aatagaagca acatgtgcag
    47341 gaaatagttt tactaattct tgtcatcgta ttaatgcaaa accaagtacc aaaataatct
    47401 ctgttttaat aaaaaaagca gataagcaag tagctgaagc tttaagtatt gaactagaag
    47461 agaaggtaat atgtattaaa agacttagat taatagatga agtacctgtt atttttgaag
    47521 ttgactattt tagaatagat tacatgtttt tattaaaaga agaattagaa gataaatctt
    47581 taatggaggt tatatctaat aatataagca cattacctaa acgagtagaa aatatatttg
    47641 aagtaaaaca ttcaaacaaa gaatattctg accatcttaa atgtgcaagt aatatgccac
    47701 tattgaaagt aagacaatcg gtgtacacag aaaataatga tgtcttatat tacaatgagc
    47761 aatttataag aagtgataag tacaaatatg cagtatcagc agaaatataa tctataattt
    47821 agtaggagga tgagtatgaa aaagataata ttagcatctc atggagactt ttcaaaaggg
    47881 cttttaaatg cagtaaaaat gatagtagga gatttagcag attgtgtcag ttcgtatggt
    47941 ttgtaccctg gacaaagtgc ttcagatttt gctttagaat tagaaaaaac aatatcagaa
    48001 gataaagaat gtgaatatat aattttatct gacttatatg gagcaagtgt atgtacagca
    48061 atgttaagat taactaatct acataatgtt aggttatttt ctggaatgaa tttgaatatg
    48121 gtattagaac ttcttacaag gttttctgat gaattaacag ttgaagatat agaccaatta
    48181 gtggaagaat ctagaagagg aatacaaagc gtgacattac aaattaaaga agaagaagag
    48241 gtattttagt ttatgaaaaa aaagggaata ttatcagtat ttaaaagtaa aaaaccaatt
    48301 atagcaatga tacacttaaa aggagatact ccagaagata tctttgaaag agctaaaaaa
    48361 gagattacaa tctttgaaga aaatggtgta gatggaataa tgttagaaaa ttactatggt
    48421 aattattatg atttagaaag aatacttgaa tatgtatcaa aagctaattt atcaatacca
    48481 tatggagtaa actgtttaaa tgttgatact atgggatttg aactggcaac aaaatataat
    48541 gctagttata tacaggttga ttctgtagta ggtcatgtta aacctagaga tgaagctact
    48601 ttagaagagt tttttaaatt acaacgttca aaatgcccag cgtatttaat tggaggcgta
    48661 cgttttaaat atcagccagt attatcagaa aatgatgttg aagaagattt aaaaatagga
    48721 atgacaagat gtgatgctat agcagttaca gaaaatgcta caggtcaaga aacatcaatg
    48781 gaaaaaatag aactgtttcg taaaaattta ggagacttcc cattggttat agctgctgga
    48841 gttacattag aaaatgcaaa aaaacagctt aaattaggag atatggcaat aataggaagt
    48901 tactttaaag ataattacaa agattttggg gatgttagtg tagaacacgt aaaaacattc
    48961 atggatgaaa tcaaaaaaat tagggaggaa ttataatgat taaattagta agagtagacc
    49021 acagacttat acatggacaa gtagcattta catggacaaa gtttttaagt acagactgta
    49081 tattgatagc aagtgatgac ttattaaaag atgaattaag aatggcagga cttaaaatgg
    49141 ctaaaccatc taatgttaag ttagtaatga aaagcatagc agattctata aaagcactta
    49201 actcaggtgt tactgataaa tacaacttat tgatactttg tgaatctgta gaagatgttt
    49261 acagacttgc taaagaggta aaagctatta aatctataaa ccttggtgga acaaaatcag
    49321 atgataatcg tgaaaatata tctaaagcag tgcatgtatc aaaagatgat ataaagatga
    49381 ttaaagagtt agattcagaa ggcgtaaatg tatttgtaca attagttcct gatgatgatg
    49441 ctacaaatgt aatgaaatta atataatttt agggggatga aaatggaatt tacacaagtt
    49501 attttgataa ctctaattgc attctttgca tatatgcata gttttgtagg ttctacaatg
    49561 cataatagac ctatagttgt tgcaccactt gttggtttag cactaggtaa tcttcataca
    49621 ggtatagtaa taggttcaac attagaatta gtatttatgg gtgcatttcc tgttggggca
    49681 agtaatcctc ctgattttgt gtctggttct attattgcca cagcgtttgt aatattaacg
    49741 ggtcaagatg tatcagcagc agttgtactt gcagttccta tagcaactct agtattgctt
    49801 atagataact tcttaatgac agtagtactt acatggggag ctcatattgc tgaccgttat
    49861 gcagaagagg ggaatattaa aggcgtagaa cgtgtgcaat tattatttgg tataggaaat
    49921 aaattaatat tagcaataat agtaggtata ggattttcat taggtgttcc agtaatagaa
    49981 aaaatacttt catttatacc atcatatgta actcatggaa tggatgttgc agcaggcgtg
    50041 attccagcaa taggttttgc catgttagct agaatgatgc ttaataagaa aacagtagca
    50101 tttttattat taggatttat attggttgct tacctaaaca taacagtaac aggagttgca
    50161 ttgtttggat tagcaatagc tttaatatat gttaactttg taggagagaa ggaggtaata
    50221 gtagatgaca acgagttcta agaagttaga gacaatttca ccagatagta aaataacgag
    50281 gaaagacttt tggaaatgtt ttagaaggtc attgacatta gattcatctt ggaactacga
    50341 acgtatgcaa aatatagcat atgcatacat gatggcacca ataatccgta gattatataa
    50401 agatgataaa gaaaagaaat ctaaggcttt aaaaagacat ttagaattta tgtcagttac
    50461 tcctcatata agtactcttt tggtaggtat atctggagct atggaagaag aaaatgcaaa
    50521 gaataaagaa tttgatgcta atagtataaa tgctgttaaa tcaagtttaa tgggtccagt
    50581 ttctgggata ggagattcat ttttctgggg aacattaaag cttatagcag ctggggttgg
    50641 tatagcttta gcttctcagg gtaacataat gggaccgatt ttattccttt taataataaa
    50701 tgttcctcat tttattatac gctatatatg tctagataaa ggatttaagt atggaactca
    50761 attctttaaa gatgtgagtg gttcaagtat agtttcaaaa gttatggaag cagcttcaat
    50821 gttagggctt atggtaattg gaggtatgac tgcatctaat gtaatgttaa aacttagtgt
    50881 taatgtaggt agtggagaat gggcagagcc tattcaaact tacttagacc aaataatgcc
    50941 atgtatgctt ccagctatga tattcggtat tatgtattgg ttattaggaa aaaaggtaaa
    51001 gacaactacg atattaattt cagttatgat tatttgtata gtattggctg ctattggagt
    51061 agtataaata aaaaaatccc aagttcattt gaacgaggga ttttttgact aaattaaata
    51121 aaaaagaaag aaggttcata ataatttata agcatttatg tgatagttat aattagctta
    51181 ttaattagtt ataaactcaa aattaagcac tactttatta aagttattat attataattt
    51241 cgaaatactc acataatctg tttagaaatt tatcttctaa tggggaaaaa gttttctttt
    51301 tagagtaaat gaagaaaaac tttctagtaa aatcaacatc ctttatttta taacatttta
    51361 ttttacctgc attgagataa tcaatagctg aaatgtacga tataaaggat actccaagac
    51421 ctaatctaac catttcctta atagcctcat ttgattctac atgagcgatt acatttaatt
    51481 tacttactgg aaagttattt ttactaagtg tgtttagtat taagtttctt gtacctgacc
    51541 cttcttttct cataataaaa tttagataag ccaattctcc tatatcgata caattatttt
    51601 tattatcaat tttaaaatct gaaggagtga taagaacaag ttcatcatct aataagtcta
    51661 ggtactctat ttgaggatta ttgattttag aaccaactaa tccaaagctt attcgttcat
    51721 ttaaaatttc tgatatagca tattgagaat cataatggct tatggaaaat tttacatctg
    51781 gataactcat agaaaaactc ttcataaaat caggtaaaat ataagtttca ggtatggaac
    51841 tacatgctat atctataata ccttctattt ttcctgaata ttcttttata tcataaattg
    51901 cacgtttgca attattcaat atatatatag catgttcata aagaatttca ccagattttg
    51961 tgagtgtaat aactttgttg cttctattta taagaacagt ttctagctct ctctctaaat
    52021 tctgtatgtg tgcgcttaca gtaggttgag ttaaaaaaag ttctcttgca gcctttgaaa
    52081 aactctgatg ttttgcaaca gcaacaaata cttcaagttg tttaaaatcc attatttaat
    52141 cctccttaaa ttatctaaaa ctattataac atatttgata aattaatagt caaaggtgat
    52201 ttttaaaata gaaaaccttt aaatattcat ggagtttttg attgtttagg aataatttgg
    52261 tataatgttg taaggtactt acaataaaaa tatcgtaaat tgaattttca aaattcaact
    52321 ttaaagtgtt ttttattaca gaaaaattac aattagcttc tt
//
LOCUS       DAEMHW010000005        47336 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEMHW010000005 DAEMHW010000000
VERSION     DAEMHW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.005"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1..1337
                     /locus_tag="KPK67_000215"
                     /pseudo
     CDS             1..1337
                     /locus_tag="KPK67_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            complement(1460..2614)
                     /locus_tag="KPK67_000216"
     CDS             complement(1460..2614)
                     /locus_tag="KPK67_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBG2457238.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVKNLLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(2874..5489)
                     /gene="mngB"
                     /locus_tag="KPK67_000217"
     CDS             complement(2874..5489)
                     /gene="mngB"
                     /locus_tag="KPK67_000217"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG2457239.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHHEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMSRRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKQEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESILIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(5633..7558)
                     /gene="mngA"
                     /locus_tag="KPK67_000218"
     CDS             complement(5633..7558)
                     /gene="mngA"
                     /locus_tag="KPK67_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBG2457240.1"
                     /translation="MEFKKITNENLVFLKQKIKTKKDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVEII
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(7688..8413)
                     /locus_tag="KPK67_000219"
     CDS             complement(7688..8413)
                     /locus_tag="KPK67_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2457241.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(9056..9826)
                     /locus_tag="KPK67_000220"
     CDS             complement(9056..9826)
                     /locus_tag="KPK67_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBG2457242.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKIWLRRNNIELVDFRDLLKNF"
     gene            complement(9819..11246)
                     /locus_tag="KPK67_000221"
     CDS             complement(9819..11246)
                     /locus_tag="KPK67_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2457243.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPIGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            11637..12359
                     /locus_tag="KPK67_000222"
     CDS             11637..12359
                     /locus_tag="KPK67_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2457244.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            complement(12507..13688)
                     /locus_tag="KPK67_000223"
     CDS             complement(12507..13688)
                     /locus_tag="KPK67_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2457245.1"
                     /translation="MKHNFDEVYNRKGTYCTQWDYIQDRFGKKDLIPFSISDTDFTVP
                     KPVYEKLQEILQHQIYGYSRWNHNDFKGSIAGYYNRRFEAKIESNWVLYSPSVMYSVA
                     LLLRLLCDNKKKVATFNPMYDAFFSVIEENGFELEKIQLYEKDGKFNIDFDEFEDSVK
                     NCDVLLLCSPHNPTGKVWKEVELEFLINTCKKYNIKIISDEIHADIIMPDNKHLPIVK
                     YLREYKELYLVSSGSKTFNYPGLIGSYAVIANKNIYDEFLSITRRRDFLNSVSLPGMY
                     ATMVSYNECDYYIEQLVEYIDNNLSYVSQFIKENFEDIKFTKPEGTYLAWIDCRDVPF
                     TCEEIQDSLINIGGVGIMRGEVYGSEKYLRLNCGCPREKLKKGLERFKQSIDYLYFNN
                     K"
     gene            complement(13909..15246)
                     /locus_tag="KPK67_000224"
     CDS             complement(13909..15246)
                     /locus_tag="KPK67_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2457246.1"
                     /translation="MFKKFEQFMNKHLTPIAQRMDKQHHLSAIKKSMVAMTPILIIGS
                     LSLMPEAIGNLTEKAIGANAITSFITNNMDSLQIPFYLTIGMMGFLVCMCITYFLSEH
                     YKLYVPGAMSLAAVGFLLMSFNFTEDGGMDIKYMGTKGLFIAMVAGVLAVELYRWCKK
                     KNFTIRMPESVPDFVSSSFELIPTTVIIVGVFIGIRIITLQVFGVLPPEILTQFLAPL
                     VGSLDNPFTFAFITFLGITLFFFGIHPSVLSPITAPITAQFLAENLEAMAVGQSLPHF
                     YTGGMVSAFANFTGTGVTFGLVFWYLMSKRVDFKRIGQVSLVPALFGINEPILFGGPI
                     VLNPTFFLPFVIGGTILGTFPAFLMNAGILAKPFFNPPYLGVFIEGFLVNGHWLTPIV
                     QAFQMVGSILIWYPFYKMYEKQCDERDTKQVQEIQEESVISPEDLGLLGDLDF"
     gene            complement(15285..15611)
                     /locus_tag="KPK67_000225"
     CDS             complement(15285..15611)
                     /locus_tag="KPK67_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2457247.1"
                     /translation="MRIILCCAGGFSTTMLMKNMENIVKNSKKLNDDDFEFKAIPVDL
                     LKNEVDNCDVLVIGPQIAHRLDYIKPILEPKNVPYVIIDQNTYGKMDGATALKMALIA
                     HKKANM"
     gene            complement(15614..15934)
                     /locus_tag="KPK67_000226"
     CDS             complement(15614..15934)
                     /locus_tag="KPK67_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2457248.1"
                     /translation="MENLEMKIMEIIVSSGDSKSKSFEALNKVKEGDYEGARACIEEA
                     RKIDIEAHNAQTALIQSELSGDCESKSMVSLLMVHAQDHYMTSQLSRDLIEKLIDIFE
                     SKEA"
     gene            complement(15952..16980)
                     /gene="ypdE"
                     /locus_tag="KPK67_000227"
     CDS             complement(15952..16980)
                     /gene="ypdE"
                     /locus_tag="KPK67_000227"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG2457249.1"
                     /translation="MSIDYLKKLCEADGIASNEQEIRDLLVENINGEISFDNLGSMIV
                     HHRGNGVKMMFCAHMDEVGFMVRHISDIGMLHLINVGGVKDSAKSFQKVNISKVDGTK
                     VKGLINCSYDENNKVKDLYADIGVSTAKEVEDLGINIGDMVCFASESEMLNENVLMAK
                     ALDDRIGCYILKEIDRRVNQIKKNDNDIYLVYTSSEEVGIRGGKTTSKVVNPDIVFAV
                     DVACAPDLIRNYTNQRQLSKGCMIVHYDKTMIPNKKFLNYMKDLATKYDIKFQCDMFS
                     GGGTDAGNAHLVNEGKLAIVLGVPLRYCHGNYSMVDMRDVEEVINLCTAVINDMNMEK
                     YNNILKFI"
     gene            complement(16992..18833)
                     /locus_tag="KPK67_000228"
     CDS             complement(16992..18833)
                     /locus_tag="KPK67_000228"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012579.1,HMM:NF013070.1,HMM:NF019884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2457250.1"
                     /translation="MRILKIINDLENTNKYLSIAYFIDKYSVSKRTLQNDFSYLSKVS
                     SNKGFKIIQKRGLGYLLEIEDNTLFKKFKVELFEYLDQPKLNVESIIACLILNEDYIT
                     IDRILEELKTSRSVVKGYKEDIDSYLKKYNLKLERKSHYGLKIINSMKERRKLIVELY
                     IKENEIIKKYIDSKLENDFKEVQEKFINKLKENNLVINYIELKEILSWLKVTMYIDRQ
                     SKLFELDDSTNEYELNLSSEDYKILKSLIKKKARKKFRRQKGADNLRDDLEDFLKQFD
                     EENNTYFNEDEEFKRSLLNHLTELLERSTFSISYQNPLIDEIQIKYPVAFNIAILLGK
                     IMYKKYNVEIQRDEIGFIATYFAMNMEKQAIYKFRKYNSIAIVCSSGGGSAQLIKFKI
                     SSLFENTNIKTFSLIQMKELEKFNPDIIFSICELDINLEVPIVYIKELLDDYDILRIK
                     ELIMFEKFKEYSLENKGQAFICKFLRNDYFNIDNDSIDYIKCLEDMSKNIELKGAGNY
                     GYSENVLKREYFATNIYINGVAIPHPIDIDGNENLISVKILQNPIDFKGKKVSLIFMI
                     CLKKENIGLYKKISSSLYEIMNDIETVQLLVNAKTFKEFVALINQRI"
     gene            19340..21007
                     /locus_tag="KPK67_000229"
     CDS             19340..21007
                     /locus_tag="KPK67_000229"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBG2457251.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(21103..22422)
                     /locus_tag="KPK67_000230"
     CDS             complement(21103..22422)
                     /locus_tag="KPK67_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2457252.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQVAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVDVK"
     gene            complement(22463..23209)
                     /locus_tag="KPK67_000231"
     CDS             complement(22463..23209)
                     /locus_tag="KPK67_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG2457253.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(23453..25462)
                     /locus_tag="KPK67_000232"
     CDS             complement(23453..25462)
                     /locus_tag="KPK67_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2457254.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(25934..27367)
                     /locus_tag="KPK67_000233"
     CDS             complement(25934..27367)
                     /locus_tag="KPK67_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG2457255.1"
                     /translation="MSFKKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(27383..28807)
                     /gene="ascB"
                     /locus_tag="KPK67_000234"
     CDS             complement(27383..28807)
                     /gene="ascB"
                     /locus_tag="KPK67_000234"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG2457256.1"
                     /translation="MALRKDFLWGGATAANQCEGGYKEDGRGLANVDVTPQGKDRFPV
                     LAGKMKMFECDDEHYYPAHEAIDMYHNYKEDIKLFAEMGFKCYRMSIAWTRIFPTGVE
                     DVPNEEGLKFYEDLFDECLKYGIEPVVTICHFDVPMYLVKKYGAWRNRKLVDLYVKYC
                     EVIFTRFKGKVKYWLTFNEINMILHLPFIGAGLYIEEGENEEQIKYQAAHHQLVASAL
                     VTKLAHNINQEFKIGCMLAAGNTYPNTCNPLDVWKSIEKDRENYFFIDVQSRGEYPTY
                     ALKELERKGINIVMEEDDKKILKENTVDFISFSYYSSRLTSADPKVNILTEGNVFASL
                     KNPYLDASEWGWQIDPLGLRITMNSLYDRYQKPLFIVENGLGAVDNPDENGNINDDYR
                     IDYLRAHIKAMIDAVELDGVDLWGYTPWGCIDLVSASTGEMKKRYGFIYVDKDNNGNG
                     TLKRSKKKSFAWYKKVIESNGEEL"
     gene            complement(28894..30810)
                     /locus_tag="KPK67_000235"
     CDS             complement(28894..30810)
                     /locus_tag="KPK67_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2457257.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDSIVEISKDDNRQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQELITVLA"
     gene            complement(30884..31744)
                     /locus_tag="KPK67_000236"
     CDS             complement(30884..31744)
                     /locus_tag="KPK67_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2457258.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKCIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(32095..33282)
                     /locus_tag="KPK67_000237"
     CDS             complement(32095..33282)
                     /locus_tag="KPK67_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2457259.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSRLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(33296..34687)
                     /locus_tag="KPK67_000238"
     CDS             complement(33296..34687)
                     /locus_tag="KPK67_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBG2457260.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(35282..36982)
                     /locus_tag="KPK67_000239"
     CDS             complement(35282..36982)
                     /locus_tag="KPK67_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2457261.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(36954..38357)
                     /locus_tag="KPK67_000240"
     CDS             complement(36954..38357)
                     /locus_tag="KPK67_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2457262.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNREKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(38621..39112)
                     /locus_tag="KPK67_000241"
     CDS             complement(38621..39112)
                     /locus_tag="KPK67_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2457263.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(39138..39950)
                     /locus_tag="KPK67_000242"
     CDS             complement(39138..39950)
                     /locus_tag="KPK67_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2457264.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSEEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(39963..41114)
                     /locus_tag="KPK67_000243"
     CDS             complement(39963..41114)
                     /locus_tag="KPK67_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG2457265.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKSVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            41384..42025
                     /locus_tag="KPK67_000244"
     CDS             41384..42025
                     /locus_tag="KPK67_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBG2457266.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTVDGANTFERDWSKVNK"
     gene            42051..43595
                     /locus_tag="KPK67_000245"
     CDS             42051..43595
                     /locus_tag="KPK67_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2457267.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(43795..44421)
                     /locus_tag="KPK67_000246"
     CDS             complement(43795..44421)
                     /locus_tag="KPK67_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2457268.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(44556..45272)
                     /locus_tag="KPK67_000247"
     CDS             complement(44556..45272)
                     /locus_tag="KPK67_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBG2457269.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            45709..46923
                     /locus_tag="KPK67_000248"
     CDS             45709..46923
                     /locus_tag="KPK67_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG2457270.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIISSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
BASE COUNT        15485 a         8326 c         5239 g        18286 t
ORIGIN      
        1 ttataaatga gtatgctata aacaacatac ttcttcattt aatgataact atagacagaa
       61 taaaaaagaa taattacata gactctgttg aagtagtaaa ctacatagac aataacgtag
      121 atataaatat tgcaaaagac atatcaaatt ttttagaatc tgaatacaat ataactttaa
      181 ctagtgcgga attatactat ttagtatttc agttaacaaa caaaactaca gtcttaaact
      241 acaatcaaat ggatactaaa tcattgtcaa attatataga tgaacacttt gttaaattaa
      301 ctaaaaaaat aattaaaaat gtatatgact tatatcttat agatttatct gatgaggaat
      361 ttgttgtaaa atttactctt catgtaaaaa atttaatcag tagagccaaa aataatcaag
      421 ttttaagaaa tcaaatacct caaaagttaa aagattccta ccctttgata tatgatatat
      481 ctgtttatat ttgcaatcag attcaaattt tagaaagcat agatatagac gaagatgaaa
      541 tatcgtacat atctcttcat gtaggttcat tctttgatag acaaaaatta ttagaagata
      601 aagttctatg tgcattaatt actccaaatt actatgattt acaattcaaa atagtaagag
      661 accttgagaa gagatttaat gaatccatag aaattattca gattttttca gatactcata
      721 atcttgattt tgacaataag gtagacatgg ttattactac tttaccaatt aacgatagat
      781 gccctatacc ttttgtttat gtaaatcctt acctaaatag aaaggattat gataatatcc
      841 aaagtaaatt cattcagatt aaagatagaa aaaacatact tactgtacaa aatcatttag
      901 aaatgtactt tagtgaatct ttgtttatga aaaatatata tttagatagt gctaaagatt
      961 atataaaatt tatgggaaat attctatacg aaaataaata tgtcaaaccc aattatatag
     1021 acgatgttct tataagagaa aaaatgtctt caactgcatt taataacaat gtagcaattc
     1081 ctcatagcat gaaaatggat gctttaaaaa ccggagtatg tttaattgtt aacgataaac
     1141 cagttaaatg gggtgaataa aaagttcaaa ttatagctat gatacctata aatgaaaaag
     1201 aaaaagaaaa attcaattac atatttgaaa gctttattga aatattatct gagtggaata
     1261 atgttaaaga acttactaag gcagataact atagtagttt tatgaataga atagcttatc
     1321 ttattcaaaa tatctaaatc cattaattta tttccaatag atgtttctta agttattaac
     1381 acaaaataga taagctgtca ccctaaaaag tctaaattaa tatgtgacag ctttttacct
     1441 aatatacctt gttttaatac tacatatttt ttgactgttg tatactagtt ataagattga
     1501 atatttgtgt tgttgtagct gtcatattct cactagcagt tgctttatcc attgcttctt
     1561 ctattgtcat agctctattt actatagaaa aataagcatc tataccctct tcattacatt
     1621 taactgcatc ttctgttgtg gctccagcca aacctattac ttttacccca tatttttttg
     1681 ccagttttgc aactcctatt ggagccttgc ccatagcagt ttgattgtcc aatctacctt
     1741 caccagttat gacaaaatct gcatctttta actcttcctc taatttaatt tcttctaata
     1801 ttatttttat tccagattct aattttgaat ttaaaaatgc taaaaaagca aaacctaatc
     1861 ctcctgctgc tcctgctcct tctacattag ctacatcttt acttaagtaa tttttaacta
     1921 ctttggaaaa atttctaagc ccattatcta actcctcaat tatttctgat gttgcacctt
     1981 tttgagaccc gtatatgtat gcagctccat ttttcccaaa cagcagattc tttacatcac
     2041 aggctatttt aaacttacat tcatctaatt cctttaatct attttctatt tttatatgtc
     2101 ttatttcatt taaaactttt ccacctaatc ctactaactt gttattttca tcataaaact
     2161 caaaccctaa ggcttgaagc atacctatcc cagcatcatt agtagcactt ccacctattc
     2221 caactataaa atttctatag cctttattta ttgcttcctt tattatttcc ccaacaccat
     2281 aagttgtagt gtataaagga tttctaagtt cagtcggaac caatgttaat cctgatgctt
     2341 gagctatttc tattattgct gtacttgtat tttttaataa tccatatgtt gcatccactt
     2401 ttttagctat tggtccagta acttctatag tctctttttt accattcatc ccttgaacta
     2461 aggcatctac tgtcccttca ccaccatcag caagtggcaa tatctttact tgcgcatctt
     2521 ctttaacctt caatatcccc ttttttattg catttcctgc ctctattgaa gataagcttc
     2581 ctttcaaaga atctatagaa attaatattt tcatatttat ctactccttt ttattactcc
     2641 ttattttaaa catataattt tataagctta atttatcttt tattttatac tctacatctc
     2701 tctttgtata caacttatac cattaatatt cttttatctt ataaaaaggg agtgtctcaa
     2761 aataaatttc ttagttcatg agacactctt tttgtataaa atcaaatata tgtttattat
     2821 ttcaacaatg ataaggagga ttatctctat tttaagacaa ttccttttta gcactattta
     2881 accaatatag ttttaacttg gttcattaag cactctacat ttacagagtt attatctatt
     2941 aatatagatt ctaattcttt ttcatttagg ttaatccatt tagctgtttt aatatttttg
     3001 ttaaatacta tgttagctga ttttgtttct tctttagaat tgtaaaatct aactactaat
     3061 gcctcttgtt tttcagcctt ttttacaaca cttaacacaa gatccttatt agtttcctta
     3121 agtaaactat atgaataatc tacttctatt tctgattgat tcaatttcat agcattatat
     3181 ggcattttat tataagtttg tatttttgtt aaatactctt tagccatatt tgcaacattt
     3241 gcttctagtg tagatttttc atgagtaact attgctaagt ctatttttat atctcctatc
     3301 atttgagaat ctggagtagg caactttatt cctgatggac gacctgggcg tcttgacatt
     3361 tcttctttac ctaggaatcc tacacttcta aataatgtta tagctattgt gtcaaatttt
     3421 tcccctacta tttcaaactc tcttgtactg tttgtaagaa cagctactcc atgctctttg
     3481 tcagacaatc caacgaatgt tagcattgga tatatagaat ctggtctttc actccattct
     3541 tcctgttgcc aaacttccat agcatcatca tacacacttc tctttatatg tccaaactga
     3601 ttatcagaca cagaaaactt tgaagctata tttgttggta tataagttct aaccctacaa
     3661 tctcttgctt ggttatttat gttgaatact acatctatta tagctttatc atttggaaca
     3721 tttataacta tatcaaagtc aataaatgaa tctgctctat tattttttct agcttgtata
     3781 tcttttggta cttttaatct ataatttatc tttatctcat tattaaatct atttttcttt
     3841 atagtggaat ttgctactat attatcgtta aatattaatt tctcatttgg aagtggtgaa
     3901 aaatcatact catctccttc atctcctcca ttttccatca ataatacatt ttcaaaagtt
     3961 ttatttaatt ttttatcaaa tatctttaat gtcccgtttt cattaacttg aatagtataa
     4021 aagtcattat ctaccatgtc acatacttca taagcttttt ctaacatatg ttcaacttct
     4081 tttatcatat acgccttata ccccattgca ggtattttat ctttaaagtt tattgtatat
     4141 accataaatg aatcatagtt tccataatga acaatttgac ggtctattaa tcctgcatct
     4201 attacttctt tatgtaccac ttcaaaatcc aacttattat tatctttatc aaatatttca
     4261 aatgctttca ttttagttat aacttgggcg cttactattt cattcctatc gtaaggcatt
     4321 aagttaaatg ctactaactt atctaaagcc atttcacaac tcattgcatc aactattctt
     4381 cttttgtaaa atgttattaa ttggtctact ttttcctcag ctaagaaaaa tctattcatt
     4441 atttcatggt gaactttatc tgaacaacaa catccaatgc tgtcatgagc atgatttttc
     4501 attatttctt tccaaattaa ctctattaat ccatgatgat attcaaatcc tagagaatat
     4561 gcaatagaag ctagtggctc caatatgttg gttatctttg attctattct agcatttgct
     4621 gattttatat ccattcttgt tgagaatata cttctatgaa ccctcatata tttcccatct
     4681 aaaaattccc cttttattgt atctatatcc tcattttttt ctagctcttt aaatatgttt
     4741 tcatatctac ttaagaaaaa ttttctctct ggataacatt cttttaattt atatataact
     4801 tcaaatatat tcttttgaat tggcatttgg tcatgtccat ttggaagtat tatatggtca
     4861 gtagttgcac ccttatcaag aaccggtaaa tatttatcca ttcttgttct tagctcatct
     4921 tcattcattg gtagatattt tcctattgca taacctaatg gaagtatttg aaccaaaaca
     4981 gaagacccat catcactctt ccaattaaac tctgttttat tagttccttt tctttcagaa
     5041 catcctctcc aaaacattga gtattttata tcaaacccat ttagtatttg tggcatttga
     5101 gcactttgac caaatgaatc tggtaaatat cctattttca tatattctcc aaattcatca
     5161 caatctttta ttccatatag taaatttcta agtatggatt ctccaccaac taccatttca
     5221 tcagtttgag tataccaggg acctattatt agccttcctt cttgaactaa tttttttatt
     5281 ctctccttac attcaggctt aactgctaaa taatcttcta atatagcagt ttgaccatcc
     5341 atcacataat atggatagtt atgattattt tctaacatat ccattatttc ttccatatta
     5401 tttacaagta atattctaga ttcctcagtt gagaagtacc attctctatc ccaatgcata
     5461 tgaggaacta tgtgtacatt ttttttcatt gcttatcctc caaaacttat ctgataatat
     5521 tttctgtcta attaaaaatc caataaaaat actactgagt ttacaaaatt ttttaatatt
     5581 tattttatca ctataattaa acctttgatt taaatatcta ataacgtatc ttttaatgta
     5641 tctttctttg atttttttgt ttcctagtta ttattaataa tattgctgat attatagctc
     5701 caagtactgc cgcaaagaac catattgatg ctataataaa tcctgacata cttccctcta
     5761 aaagcgccaa tgagaatatc cccgctccag gcacttgaag acctatattg aagaatgcta
     5821 ctatagaccc agttatggct gaaccagcta ccaatgatgg tataacacgg attgggtcct
     5881 ctatcataaa tggtattgca ccttcagtta ttccagccaa tcctaatatc caagtttgct
     5941 ttcctatttc cttttctgat tctgaaaata aatctctgcc taaaacagta gctattgtaa
     6001 ctccaaaagc tgaaaccatc ttaactgatg caaatattgc ataaggtata aaatttccac
     6061 ttgctattgc tcctattgca aatgtatagg ctgctttatt tattggtcca cctaagtcaa
     6121 atgaaaccat agctcctatt attgcaccta gtagtattgc atttactcca gtcataccat
     6181 ttaaccaatt tataagacca ttgttaagtg ctgctacagg ctttcctact acaaataaca
     6241 tcaataaacc tactaccaaa gaaccaacta ctggatatac ccaaaaactt acgaatccag
     6301 ctaatgtgcc tttggctggt atattcttct ttaaaaattt tactatataa ccagcaagta
     6361 aacctcctaa catacctcca agaaatccac tacctattaa atcagctgct aggcctgctg
     6421 caaatcctgg tccaagacct ggtttttcag ctagtgagta agacatatat gctgataata
     6481 ctggaaccat caatgtacct aacatacctc cagaaagctt tcttaattgc cataaccaag
     6541 aaccttcttg attgtatagt tcttgtagac caaatgcttg agatgctaat actgcaaatg
     6601 caagtatcat acctcctgcc acaataagtg gtattatatg agatactcct gtaagtattg
     6661 ctgattttac atcttttttc catgatgatt tttcacaata tgatatatca gatgattttt
     6721 ctatataatc acctttacct tgtttttgtg ctacttctaa agcttcgtat attatttctt
     6781 ttgctctttt tagtggagct gctactgacg tttttatgtt tggcaaatgt tcaaatcttt
     6841 ctgtattttt aggtgctaca tctgttgcat atattattgc atctgccctt tttattatat
     6901 ctggtgtatg agggtccacc ataccattag ctccttgctt ttctacatac acatccacac
     6961 cgatttcttt accagcttta attaatgctt ctgctgacat atatgtatgt gctatacctg
     7021 cagggcatgc tgttattgct aatataattt tttttgattc attttgaatc ctaatctctt
     7081 cttttatttg ttctttcttt tcttttatat ctaagttatt aaataactct ttcttagttt
     7141 ttgaatttaa caacatattc atataatctt catttgacag tctagtaact aactcagaaa
     7201 gtatttgtat gtgtgtattt ccaccttcat tttctggtac tgctattaaa aatattatct
     7261 ctactttatt atttgggtct acgctttccc aattttctat tggtgtctct aaggtcatag
     7321 ctgcaaatgc agcttcttta actgacttag attttccatg aggaattgca agccctcttt
     7381 caaaacctgt tggagataaa gtttctctgt acattacagc ttctaaatat tcctcataag
     7441 atgttaactt gccttcatca tatagctttc ttgctaagaa ctttattgcg tctttctttg
     7501 tttttatttt ttgttttaaa aaaacaaggt tttcatttgt gattttctta aattccaaaa
     7561 ttacgccctc cttaaatcat ttaatgctat gaatatagca taaaataaat catgaaaatg
     7621 tttttttgaa attatacaca caatttcttc ttaaaaaata aagatttgtg tatttataca
     7681 caaatctcta tcttaaccag tattgttcta ttattgctct cataatcatc ataattggaa
     7741 ccttatctga tatattgtac ccatttactt gtttaattgt tgaatagcaa tataaactta
     7801 tatctgcaat ttgttgcaat tgattttcac atatatgtgt tagagctact accttacaac
     7861 ccttattaac tgctgtcttt gctgcttcta taacttcttt cgtttctcca cttaaactaa
     7921 gtgcgaaaaa catatcttgt tcacctaatt ttcctatatc atgaagtact tcatgtcttt
     7981 gactatagta agttatatta tcatgcccac ctctaatttc ttttactaat atctcacata
     8041 aaggaatgtt ttgacctatt ccataaacta taatactcct agatttttga attatatctg
     8101 ctactttatt tattttttca taatctatta gctctaatgt cttttttata tctgattcaa
     8161 aaccattttt tggctcttta tcctcatttt ttaactcttc ttttaaatta ttttttagtt
     8221 gagagaaacc atcatactcc atttttttac atagcctaac tatggtattt gggacagtaa
     8281 aaaatttatc tgctaaagat tgtattgata attttactat ttcttctttg ttttttctca
     8341 tatattcaat tatcatgtct tctgtatctg taaatttata ctcatatttt tttgctcttt
     8401 cataaaattc catagtttct cctccatcta ttttattatt cataaatata atactctgta
     8461 ttatctaaat tttttagtaa tatttttaat tttttcacat atattatacc ttttattata
     8521 cataaaaaat aagtacttcc tatattgatg gagataattt aaaagtgtgt tgtttaatat
     8581 acttagttat cttttgaata taatcgattt gacattatca ttaacagata ttagaattta
     8641 agatagatta atttttatcg caacttgata aatcataact taattctata taagtgaggt
     8701 gaaaagagta tgtttacaga tataagaagc aaacgagtta tatttgttgc tcattgtgtg
     8761 ctaaatcaaa attctatttc agatggtact gctgattatc ctagcacaaa tacggatgtt
     8821 gttcgtattc taatatattt atgttattaa caaggatgta aagcctaatt ttacaaaggt
     8881 tctacatcct atattcattt acagaagatg catttatcaa aaaaatccta aaattttgtc
     8941 agcaaactaa ttatatatct tgctatttta ttttgattta cacaatattt agttaatata
     9001 aaaccttata tgttgataga aaatcaacat gacctttaga tttaattgtt agtttttaaa
     9061 agttctttaa aaggtctcta aaatcaacca actcaatatt atttcttctt aaccatattt
     9121 taacttcctt atcacatagc attgcatgtt catatactct ctcttgaata agagaagatg
     9181 attttataag actataatct ataaatcctg gatgagtaac catcaatgtt atatctttat
     9241 ctaaatttat atatttttct tcaaataatt taacaaaaga tttatttaac ttgtaaaaat
     9301 cattctgagg atatttactt ggcatgctaa tacatgaatc caaataatag ggacaacttg
     9361 gtatgccgta ttcttttgca gcatcacaca ttgctgatat tgatacatct tcttctatac
     9421 catgtatatc aatatactca ggcaatttac tcatatacaa cttgtaagtt tcaatttgag
     9481 cacaaacttc ttcatatact tcttcataat taaaaacctc tttacctgtc agtagttgcg
     9541 ttcttcttat tttgctagaa acaaattttc caccttcatc taacaaattc tttatcttaa
     9601 tatgttcttt gcaacaaggt tctcctatta ctaaattaac atgtagacca aaacatacat
     9661 catcattttt taccatttct atagcttgtc ttgaaaaagg catattcacc atgattccag
     9721 cacttgtaac aataccgttc ttataagaag atattatccc tagtgtaaca ccttctgaat
     9781 atcctatatc atctgcccta actactaatt tcttcatttt atgcatctgc ctgctttctc
     9841 aactcatcaa gttgcatttc catctcatcc tttactttaa caacatccgt tcctatgatg
     9901 atttgaactg atgtattact caatttaatt acccctttgg ctcctgtttt tttaatatta
     9961 tcatcattca aaatagtggc atccttcact tctaaacgta atctcgtcat acaattatta
    10021 agagtcacta tattttcata accaccacaa tttttaatta tctgtttagc catatatgaa
    10081 aaattaccat gagaaagttt taaattcttt tctttttcac taactttatc acctatctct
    10141 atctcaactt cacgtcctgg tgtttgtaca ttatctttta ttattatata tttaaatgtt
    10201 acataatata atacaaagaa tacaactcct ataggtataa ctaaccacgc tttatctcca
    10261 taactaaaac ttaagacata gtcaattata cttgctccat taaatgaacc caagcgagca
    10321 cccagtaaat atagtacaac tccacctaga ccagaataaa aagaatgaat tatatataac
    10381 tttggagatg caaataaaaa cgaaaattct attggttcag ttactgttgc cataaaagaa
    10441 gtcaatgctc cagcggtcat tgcacctttt acttcttgct tctttttatc ttgtgctgtt
    10501 ttatatatag cataagaaat agcaggtatt gaaaacatca taattatata aaatcctgtc
    10561 cagaagaatc ctgcatttgg gtctcctgcc ataaatctag gaatttctcc tcttataact
    10621 tctcctgttg atgttgtata ttcacccatt tcaaataata tgtaagcatt taaaacttga
    10681 tgtaacccag ttggtattag tgcacggtta cagaataaaa acaagaatat tcctattgca
    10741 ccagctcctc ccaaccaaaa accaaattga ttcattgcag cttgtggata tatccataat
    10801 aaactaaata tatatgataa aattaaagca accactggtg atagtgtaat tacacatttt
    10861 tctcctgcaa agaagcttaa aaatgctgga actttaactt tatatgtttt attgtaagtc
    10921 catgctatac aagctccaac aattatccca ccaaatacac ctgcatttaa tgtatcattc
    10981 aataactcta aatttgactt aaatatcata tatgcaacta cggctgcaac cattacactt
    11041 gtcttgtctt tagactttga gtaggcacta gctgctccta ataaaaataa gtagtccaca
    11101 tttgaaaata ctatattaga tatattagat atagctggca cattgagcat atttggacca
    11161 aataataatc ctaatatacc tgctgctggc attacagcta ttggcaccag catagtcttt
    11221 cctaaaagtg acagttgttg catcattttg tttaagaatc tcttcatatc caatcccctt
    11281 tctatttcgg tttaattttt tgtgaaaacg atttattcac attttatatt ttaaatatta
    11341 tatttgttta aacaaattgt caatattttt tattcatttt atttttatca tttagtttaa
    11401 catataaatc cttttaagtg tattgttata tctagtctat ttttttaatt catttttttt
    11461 agtttaaaaa tatgtttaac tatgtatttg aaacaaccca caaaataatt ctattttgtc
    11521 ctttttatgg ttgatatgat tctattttca ctatacatgt ctaaacacat ttgatataat
    11581 ttgttcatat cagttcaata atattactaa agacctttaa ggagggatag gggtatatgg
    11641 ctaaaaataa attttcagag atatatgaag tattgaaaga ggaaatactt gatggtaaat
    11701 atacaagtaa tatgatgctg cctactgaac ttcaacttat tgaaaggttt tcatgtagca
    11761 gaaacactgt acgtagagca atttctcaat taaacacaga aggatatgtt caaagtataa
    11821 aaggcaaggg tgttgtagtg ttagaaaata gttgttctaa tgattttttt ctaaatatgc
    11881 acaatttcaa aggtgtagaa tcgattgtag aagataaaaa ggttaatact gctactagtg
    11941 ttctccactt cagcaaaatc ttaattgata ataagttatc taaaaagaca ggttttaaag
    12001 ttggtagtga agtatattac cttcacagac taagatatat tgataacata cctaaaattc
    12061 tcgacattaa ctattttcta tgtagtatag ttaaagacct tgatgtttct atagcacaag
    12121 gctctattta taaatatata gaagagtgtc taggtacaaa aattgtttca tccagaaaga
    12181 tatttaaaat tgaaaaagct acagagcttg agttaaaaac gctacctcta aatgattata
    12241 attgtgttgg tgttattaaa aatagtgtat atactgatga tggaaagctt tttgaatata
    12301 ctgaatcaaa gcatacacct gaaacatttg tattcatgga tgttactcaa agatattaaa
    12361 agaggatgtc tcaaaataaa tatttgaatc atcttttttt attttaccaa attaaaatta
    12421 ttcattttag aaatcaattt tttatcaatt gaaaaataga taggaacaaa ttgtccctat
    12481 ctatttttaa tagtttattt tttaaactat ttgttgttaa agtataaata gtctatactc
    12541 tgtttaaatc tttcaagacc tttttttaat ttttctcttg gacagccaca atttaatctt
    12601 aaatatttct cacttccata tacttctccc cgcattattc ccactccccc aatattaatt
    12661 aaagaatctt gtatttcttc acaagtaaat ggaacgtctc tacaatctat ccatgccaaa
    12721 taagtacctt ctggctttgt aaatttaata tcttcaaaat tctcttttat aaactgtgat
    12781 acataactta agttattatc tatgtattcg acgagttgtt ctatataata atcacattca
    12841 ttataagaaa ccattgtcgc atacatacct ggtaaactta cagagtttag aaaatctctt
    12901 cttcttgtta ttgataaaaa ttcatcatat atatttttat ttgctataac agcatatgaa
    12961 cctattaacc ctggataatt aaatgtttta cttccagaag acactaaata tagttcttta
    13021 tattccctta aatatttaac tattggcaaa tgtttattat ctggcatgat tatatcagca
    13081 tgtatttcat cacttataat ttttatattg tattttttac aagtatttat taaaaactct
    13141 aattcaactt ctttccaaac cttacctgtt ggattatgag gtgagcataa taataataca
    13201 tcacaatttt ttacactatc ttcaaattcg tcaaaatcta tattaaattt accatccttt
    13261 tcatataatt gtattttttc taactcaaat ccattttctt ctattacact gaaaaatgca
    13321 tcatacatag ggttaaatgt agcaactttc tttttattat cacaaagtaa tctaagtagt
    13381 aatgctactg aatacataac tgatggacta tataataccc aattgctctc tatttttgct
    13441 tcaaatcgtc tattataata gcctgcaata cttcctttaa aatcattgtg attccatcta
    13501 gaatatccat atatctgatg ctgtaatatc tcctgtaact tttcatatac tggtttaggt
    13561 actgtaaaat ctgtatctga aatagaaaat ggaatcaaat cttttttacc aaatctatct
    13621 tgtatataat cccactgtgt acaataagta cctttgcggt tataaacttc atcaaaattg
    13681 tgcttcatat taatcctcca atataattct tctcacatac aatataaaaa tacgatttat
    13741 tagattttta tggagtattt tttgcttcat ttgcatgttt tttatctaaa atcaaatatt
    13801 aattatattt tgttactaaa atatctttct actctataaa ataaaagcta tgaatataga
    13861 tttgtaactc tatttccaca gcttttattt acatgcttga ttatatttct agaaatctaa
    13921 atcacctaat aagcctaaat cttctggaga tattacagat tcttcttgaa tttcttgaac
    13981 ctgcttagta tctctctcat cacactgttt ttcatacatt ttatagaatg gataccatat
    14041 taatatagaa cccaccattt gaaatgcttg aactattgga gttaaccaat gtccatttac
    14101 taagaatcct tctataaata ctcctaaata tggagggttg aaaaagggtt tggctaatat
    14161 tcctgcattc attaaaaatg cagggaatgt tcccagtata gttcctccaa ttacaaaagg
    14221 taaaaagaat gttgggttta aaactatcgg accaccaaat aatataggtt catttatacc
    14281 aaataatgct ggtactaaag aaacttgacc tatacgcttg aaatctactc tcttagacat
    14341 taagtaccaa aataccaagc caaatgttac tcctgtacct gtaaaatttg caaatgcact
    14401 taccattcct cctgtataaa agtgtggtaa tgattgacct actgccatag cctccaagtt
    14461 ttcagctaaa aattgagctg ttattggtgc tgttattggc gataatacag atggatgtat
    14521 tccaaaaaag aataatgtta ttcctaggaa tgttataaat gcaaatgtaa atggattatc
    14581 taaagaacca actaatggag ctaaaaattg ggtaagtatt tctggaggaa gtactccaaa
    14641 tacttgtaaa gttattattc ttatacctat aaatactcca actattatta ctgttgtagg
    14701 tattaattca aatgaacttg atacaaaatc gggtacactt tctggcatac gtatagtaaa
    14761 gtttttcttt ttacaccatc tatacaactc aacagctaat acacctgcca ccattgctat
    14821 aaataaccct ttagttccca tatacttaat atccattcca ccatcttcag tgaaattaaa
    14881 actcattaaa agaaaaccaa cagcagctaa ggacattgct cctggtacat aaagcttata
    14941 gtgttctgaa aggaaatacg ttatacacat acatactaaa aatcccatca ttcctatagt
    15001 taagtaaaat ggaatttgta atgaatccat attatttgtt ataaatgaag ttatagcatt
    15061 tgctcctata gctttttctg ttaagtttcc tatagcctct ggcataagac ttaaacttcc
    15121 tattataagg attggagtca tcgccaccat tgacttttta attgcactta agtgatgctg
    15181 tttgtccatt ctttgtgcaa taggtgtaag atgcttattc ataaattgtt cgaacttttt
    15241 aaacattatt tttcccccat catcatagta tactttaatg aatattacat attagctttt
    15301 ttgtgagcta ttaatgccat cttaagtgca gttgcaccat ccatttttcc atatgtattt
    15361 tgatctatta ttacatatgg tacattttta ggctctagta taggttttat gtaatcaagt
    15421 ctatgagcta tttgtggtcc tataactagt acatcacaat tatcaacctc attttttaat
    15481 aaatctactg gtattgcttt aaattcaaaa tcatcatcat ttaatttttt actatttttt
    15541 acaatattct ccatattttt cattagcatt gtagtcgaaa atccacctgc acaacataat
    15601 attattctca tttttacgcc tccttagatt caaatatatc tattaatttt tctattaaat
    15661 ctctagataa ctgtgaagtc atgtaatgat cttgggcatg taccattaat aaactcacca
    15721 ttgacttact ttcacaatct ccagataatt cactttgtat taatgcagtt tgtgcattat
    15781 gagcttctat atctattttt ctagcttctt ctatacaagc tctagctcct tcataatcgc
    15841 cttctttcac tttatttaaa gcttcaaatg actttgattt actatcccct gaagaaacta
    15901 ttatctccat tattttcatc tctaaatttt ccatgcttac ctcctatgtc gttaaataaa
    15961 ctttaatatg ttattgtact tctccatatt catatcatta ataacagcag tacataaatt
    16021 gataacctct tcaacatctc tcatatctac cattgaataa tttccatgac agtatcttaa
    16081 aggaaccccc aaaactatag ctaattttcc ttcatttact agatgtgcat ttccagcatc
    16141 tgttcctcca ccactaaaca tatcgcattg gaattttata tcatacttag ttgctaagtc
    16201 tttcatataa tttaaaaatt ttttattagg aatcattgtt ttatcataat gcactatcat
    16261 acaaccttta gataattggc gttgattggt ataatttcta attaaatctg gtgcacaagc
    16321 aacatctaca gcaaacacta tatcaggatt cactactttt gatgttgttt tacctcctct
    16381 tattccaact tcttcagaac ttgtatatac aagatatata tcattatcat tcttttttat
    16441 ttgattaact cttctatcta tctctttaag tatatagcat cctattctat catctaaagc
    16501 ctttgccata agtacatttt catttaacat ttcactctca ctagcaaaac ataccatatc
    16561 tcctatgttt atacctaaat cctcaacttc tttagctgta cttactccta tatctgcata
    16621 taaatctttt actttgttat tttcgtcata tgaacaattt ataagccctt ttaccttagt
    16681 accatcaact ttacttatat ttactttttg gaaagacttt gcactatctt taactccacc
    16741 aacattaatt aaatgtagca ttccaatatc acttatgtgc cttaccataa atccaacttc
    16801 atccatatga gcacaaaaca tcatttttac tccattacct ctatggtgaa ctatcatact
    16861 tcctaagtta tcaaaactaa tttctccatt tatattttca actaataggt ctcttatttc
    16921 ttgttcattt gaagctattc catctgcttc acatagtttt tttaaataat ctatactcat
    16981 acgattaccc tctatattct ttgatttatt aaagctacaa attctttgaa tgttttagca
    17041 tttacaagta gttgaacagt ttctatatca ttcattattt catataaact actactaatt
    17101 tttttataaa gtccaatatt ttcttttttt aaacaaatca taaatattaa agaaactttt
    17161 ttacctttaa agtctattgg attttgtaaa atttttactg aaattaaatt ttcatttcca
    17221 tcaatatcta taggatgagg tattgcaact ccatttatat atatatttgt tgcaaagtac
    17281 tctcttttca aaacattttc agagtaccca taatttcctg caccttttaa ttctatattt
    17341 ttagacatat cttctaaaca tttgatataa tcaattgaat cattgtctat attaaaatag
    17401 tcatttctta aaaatttgca gataaatgct tgacctttat tttctaaaga gtattcctta
    17461 aatttttcaa acataattaa ctctttgatt cttaaaatat cataatcatc taaaagttct
    17521 tttatatata caattggtac ttctagattt atatctagtt cacaaatcga aaagataata
    17581 tctggattaa atttttctaa ttctttcatc tgtataagag aaaaagtttt tatattagta
    17641 ttttcaaaca aactgcttat cttaaatttt attaactgag cacttccacc acctgatgaa
    17701 catactattg ctattgaatt atattttcta aatttataga tagcttgctt ttccatattc
    17761 atagcaaaat atgtagctat aaacccaatt tcatctcgtt ggatttcaac attatacttt
    17821 ttatacatta tttttcctag taatatagct atattaaaag caactggata tttgatttgt
    17881 atttcatcta taagcggatt ttgataagat atactaaaag tagacctttc taataattct
    17941 gtcaaatgat ttaataatga ccttttaaat tcttcatctt cattaaaata tgtattattt
    18001 tcttcatcaa attgcttcaa aaaatcttct aaatcatctc ttaagttatc tgcacctttt
    18061 tgtcttctaa atttttttct agcttttttc tttatcaaag atttcaatat cttataatct
    18121 tcactagata aatttaattc atactcattg gtactatcat ctaattcgaa taatttagat
    18181 tgcctatcta tatacatagt tacttttaac caagacagta tttctttcaa ttcaatataa
    18241 tttatgacta aattattttc ttttaattta ttgataaatt tctcctgaac ttctttaaaa
    18301 tcgttttcca gtttactatc aatatacttt tttataattt cattttcttt tatataaagc
    18361 tctactatca attttcttct ttctttcata ctatttataa ttttcaatcc atagtgagat
    18421 tttctttcta gcttaagatt gtattttttt aaataagaat caatatcttc tttatatcct
    18481 tttactactg acctacttgt ttttaattct tctaatatcc tatctatagt tatataatct
    18541 tcatttaata taagacatgc tattatactt tcaacattta acttaggttg gtctaaatac
    18601 tcaaataact caactttaaa ttttttaaat aaagtattat cttcaatctc taatagataa
    18661 cctaatcctc ttttttgtat tattttaaaa cctttattac tagatacctt agataaatac
    18721 gaaaagtcat tttgtagtgt tcttttagat acagaatact tatctataaa ataagctata
    18781 gatagatact tatttgtgtt ttctaaatca tttataattt ttaatattcg cattcttaca
    18841 cctcctatta tccataacag ttttattttt acattctaaa aaatattgta gaactatttt
    18901 attttaattg tacaatctag tatcgatttg tgtaagatgc ttatactgtt attatatttt
    18961 ctatacaata cctttaaaag gtttttaatt tcattataaa atgaaattaa aaacctaata
    19021 cattacctat tattgaagat attgtgtata tttactagaa tttaagtatc atctattatt
    19081 tgcatttaat aatacaacaa gatatttttt gaaataagag ttaaataata gtacaacctt
    19141 ttattgcatt taaatattat caaaacaaaa ataaatgtac tatgctattt atcccatact
    19201 aacctcacta ttctatatac aattatatgt ttatacaaat aattatttat tgtatatttt
    19261 agctttaata tcagtttaat acttgacaat ttgtttaaac atattataat taacttataa
    19321 attacaggag gtatcaatta tgaaaaattg gtggaaaaaa gcaactgtct atcaaatata
    19381 tccaaaaagt ttcaaagata gcaacaatga tggtataggt gatataaatg gtattataga
    19441 aaaattagat tatctgtatt ctttaggtgt tgatttatta tggttgactc ctatgtacgt
    19501 ttctcctcaa agagataatg gatatgatat agaagattat tataatatag acccaaaata
    19561 tgggacaatg agtgactttg aaaaactctt aaaagaagct cataaaagag acataaaaat
    19621 aatgatggat atggtcctaa accatacatc tactgaacat aaatggttta aagaatctaa
    19681 aaaaagcaaa gacaatccat atcgtgatta ttatttctgg aaagatgcta aacctgatgg
    19741 ttctgttcct aataactgga tatccagatt tagtggaact gcttggaaat atgatgaaac
    19801 cactaaccaa tattatttac atttatttga agaaacacaa gctgatttaa attgggaaaa
    19861 tgaaaaggtt cgtgaagaat gttacaaaat actagaattt tgggctgata aaggtattga
    19921 tggttttcgt ttagatgtag tcaacctact ctccaaaaca cccggattac ctgatgaccc
    19981 tataacagga ccaaaaggag atggaagaac tcactacgct gatggtcctc gtatacacga
    20041 atatttacat aatatgaatc aaaaagtttt caaacctaaa aatatagtta ctgtaggtga
    20101 aatgtcatct actacacctg aagagtgtat caactatact agagaaaatc gagaagaact
    20161 atctatggtt tttagcttcc atcatatgaa aacagattat aaaggaggcg caaaatggac
    20221 taatgaaatg ttctctctgg ataaattaaa aaaagcacaa tctgactttc aatataaaat
    20281 gtatgaagga aaaggatgga atgctctatt ttactctaat cacgaccaac ctagagcttt
    20341 atctagattt ggagatgata gattttttcg tcaagaaagt gctaagatgt tagccataac
    20401 actatttggt ctacagggta ctacatacat atatcaaggt gaagaaatag gtatgacaaa
    20461 tgcctacttt actaaaatag atgaatatga tgatgctgaa agtaagaatg catactatca
    20521 tatgattaaa tctggtattg atgaaaagga ggcattgctt atacttcaac aaaaatccag
    20581 agataatgcc cgtactccta tgcaatggga taatactatt tataaggggt tttctaatca
    20641 taaaccgtgg ttaaaagtta ataatgacaa tattagtgta gaaaatgagc ttaatgattc
    20701 tcgttcagta ttttatactt atcaaagatt aatcaaatat cgtaaacaat atgatatttt
    20761 tacagaaggt acttatagat tgttagatga aaatcataaa aatctatttg tttatgaaag
    20821 agagtataaa aaccaaaatc ttcttgttat aagtaatttt actcatgata atgtagtata
    20881 taaattacct gaaacttatt tgaataaatt aaactacaca atacttgaaa ccaattacac
    20941 tagagatata attgaaaata aaatggaaat aaaaccttac gaaagtataa tgatatatta
    21001 taaataaagt tatttatgta aaaatcggta taacaccagg gtgttatacc gattttacta
    21061 aattcaaagt aatatttata gaatatctat agattagaat aattatttga cgtctacatc
    21121 attatatttc tttgaatata ctttagtcat atagaaatat ataggaacta caattatcat
    21181 cccacctaat ccccaaacta tcttcatagg ttcatcaata cctgctttta tcagtaataa
    21241 tatacttata ataactgcaa atattggtat aattgctcct ccaggtattc taaaatttac
    21301 atctttacca gcatacttct ttctcaatat tagtacagct aaacatgtag gaatatattg
    21361 aataaatcta gatacaacac ttatttgaga taatgttgtg aatgaacccg accatgctat
    21421 tagtaaagtt cctatgacag atactataat tgctatataa ggagctccat ttttattagt
    21481 ttttcttata gacttaggca ttaatccttg ttcaaccaaa gcagcagcag accttggtgt
    21541 aaatatggat gatgcaatat ttatacctcc aacagatact atagttccta ttgtaattat
    21601 atacccacct ataggaccta acatcttaat ggcagcatct gcaacaggag tagctgaccc
    21661 tgctaagcca ttacccaaca cccctatact tattcccagt ataagcatat atattactga
    21721 tactacaaac attaccatta ccaatgcttt aggtacattt ttcttagggt tctccatatc
    21781 ttttgctgca acagctaaag attcaaatcc tgtaaaagca taaaataaag ttatagatac
    21841 tgctacaaaa gctgatgacg cactttgacc tttttgtatt attacaaatg gggtaaaatt
    21901 agaacccttt ataaagaata atcctacagc tataaataaa actattggta ataattttcc
    21961 tatagtcatg atattattta agtgcttaga ttgtttcatt cccataaaat ttataatccc
    22021 aagtcctaca actaatatac caattattat atttttagta agtggttctg ctacttgtgg
    22081 ccatacagat gatagtgtta cagcaaagaa atttgccata gtagcccaag caatcatact
    22141 aactatccac ttcataaatc caacttcaaa tccaacaaaa tctccaaatg cctcttttgc
    22201 ataaagatag ggccctccat cagtatcaaa ccaactacct gcttcagcaa aacataaagc
    22261 aatagaaaca gctaaaagca tacaaaaaac taatactaat atacttgctg gacctatatc
    22321 tgtataagca tcaccaggta acccgaatat acctgaccct attattgcat tgattcctaa
    22381 tagtacaata ctccaaaaac ctaacttaat gcctttttcc attgttattc cccccaaagc
    22441 caacttaata tatttgtatt ttttatttag atgcttcttt aactattgcc ataaatattt
    22501 ttttcatatt atcataatct ctagtcatca tttctggatg ccattgtatc cctattacaa
    22561 actcacttcc ttccttttct atagcttcaa ttaaaccgtc tttagatgta gctgaaacaa
    22621 tatatcccgg cgctactttg ttgacaacta agtgatgaaa actattaact aaaacttctt
    22681 tttctcctag tatttcatat aattttgtcc cctcctttat ctgtactgta tgtgtaggta
    22741 cattagatag atgttgctga ttgtgtttaa tataagcacc ttcaattaaa gataaatctt
    22801 gatacaaact acctccttca gttacattta taatttgttc tcctctacat atacctaaaa
    22861 ctggtttttt catatctaaa gcatgtttta ataatttcat atcaaaacta tctctctttg
    22921 gtgatattgc ccccaactta ttatgaggtt cttctttcca tatgagtgga tttacatctt
    22981 gtcccccaga tagtataagc gcatctatat tagatacctg ctcctttata atctcatcat
    23041 catatacaat cggtacaata tatggtatag ctttacacat aactactgat tgtatataat
    23101 cattatttac ataagccctt tcatagccag gaaacattcc tccttcatca attagtaaat
    23161 tacctgatat tcctattatt ggatatttct ttttagccaa tttcttcacc ccttaactta
    23221 tttgtgaaca ataaaaaatg ctacttctct taaagagcaa gtagcatacc aagattttat
    23281 ttatttgaac attataattt attttagaaa tacttttttt gaatacgagt aatataaata
    23341 actttattta cttatatttt attttaatcc tgtttagttt gactttttta aattgtttta
    23401 agtacaacta ataggtaaaa taggcattta gattttcctt tttatcaacc tattaaatac
    23461 tgtttaatat attttttccc cttctattta attcacttcc ctttctacca gattcacata
    23521 ttatcaaatc cagctctttt aacatattaa gtattcccct tacctcacct tctgttatat
    23581 tgtagcctaa ttcatttatt gaattaacaa taccatttct acctatattt ttattaagat
    23641 tattggcatt ttttaaacac tccattacta actttacttt ctctatagat gttctatcct
    23701 ttaatatact tatttcttta ctaaaatctt cagttatatt taataagtta tgaggtaaat
    23761 catttattaa aataacatcc tcagaactca taatgttaat atacatagca gtatttttta
    23821 attctcttat attacctggc cattcatatg cattaataat gtcttgaaca tcttgactaa
    23881 ttgctctctt ccttttcata aaatgcttta acataacttc tatatcttct tttcgctctc
    23941 ttaaaggtgg tatatttata ggcaatacat ttatcctata gtataaatct gctctaaatc
    24001 tatctccttt tatcatttct aataaatttt tatttgtagc cgcaattact ctaacatcta
    24061 tatctattac attttctcct ccaaccctca ttacttgatt ctcttgtaat actctaagta
    24121 atttgctttg taagtatata ggcatgtctc ctatttcatc caaaaatata gttccattgt
    24181 tagcctgttc aaataatcct tttttacctc cttttaaagc tcctgtaaaa gcaccttctt
    24241 catatccaaa aagttcactt tcaagtaaat tttctggcat agctgcacag tttattgcta
    24301 taaatggctg actatgtctt ggtgatgcat tatgaataga ctgagccatg agttcttttc
    24361 ccgttccact ttctcccgtt ataagaattg tataatcaga ttcagctatt ttcataccta
    24421 atttcttagt tctttccatc ttcgttgaaa ctgtttttat atcatcaaaa gtatatctag
    24481 caacttgtcc cttttctcta agcttctttg ataggttttg ctccaatttt cttatgtaag
    24541 ttatttcttg aatacaaaaa taatatccag taacccttcc aaaactctct atattatact
    24601 tatttacgtt taagtactta ttattaaatt ttataacttc atcatgtgcc ttactcaaac
    24661 taaaaatctt tttaatattc tcattctcta attcatctat atttttccct ataatattac
    24721 tttgaatatc tagtatatcc cctaaactat tattgcaaat aattatgttt cccttatctg
    24781 ataccattgc catacctaag tttgataaat ttattataga atttaattca tctgctttta
    24841 tagtaagttc tttgtattta gtatttattc ctgaatataa actaattatt tcatctgtat
    24901 acttaattag cctctcagta atttctttat catccagttt gagttttgct attatttgaa
    24961 taaacgttga actatcaata tacctatggc ctaaatcaat aacttcttct atatattttg
    25021 gaactaagct tgactctcct ggcgttatag ctatcttaat atttctatat tcttcattgg
    25081 gattgtatgg tataaaattc aaatgattta ttcctatatt gtataaatta gatattgttt
    25141 ccaatacagt atgcttatta tcgtttacta ctaatacatc tattccttct ggcaatgaaa
    25201 ataacttata tatatccttt tgctttatgc tcctatttat agttattatc tttgagacat
    25261 cttctacata tttttttatt tttaatactc tatcatctat cattaccaat attatgtcat
    25321 cttttattaa ttccttctct gttattttat ttaaataata attttttatc attactttat
    25381 ctttaaatat catttctaga tttttattta agaaacattc taatttagac aacccatcag
    25441 ttactactgc tattgttttc atttgattac tccttttaaa atatactcta ctcttttata
    25501 atatacatct gattttcact ttaatagcac aaaaaattgt acatttagta tactaacata
    25561 ttcctttatt agttttatgt ccctaaatat catagttgca atatctatca tatttcctaa
    25621 acttctatag agcaaaaatt gtgccaaatt aataattttc ttaattttat tctaaagttc
    25681 ctcaatagta tttacagact tagcacttat agcttataag tctttatatt attaatctat
    25741 ttttataggt taattgtaca aatcatatat cacttttacc cattaaatgc attataaaaa
    25801 agatggaata acgattcgtc actccacctt ttttataata aaaaattgta tatataaaca
    25861 ctatttacta ctcttctaag tacaaacagt atctacatac tataaccttt cacctacaaa
    25921 cagtatctac acactataac ctttcaccat tagattctat tacttgttta taccagtcaa
    25981 aactcttttt cttaatccta gacaagtctc cacttccatc atcatactta tccacgtata
    26041 taaacccata tctcttggcc atctcaccag tagatgcact tactaaatct atacatcccc
    26101 atggagtata tccaaataaa tcaacaccat cttctacagc ttccttcatt tgctctatat
    26161 gtgcttttaa atactctata cgatagtcat catttatcac tccattagat tcttttttat
    26221 cataagctcc taaaccattt tcaactacca tcataggtat tctatatcta tcataaattt
    26281 catttagtgc atatctaagc ccttcagggt ctatttgcca tccccattca ctagacttaa
    26341 gatatggatt tttcactcca cctaatatgt tgccacttgt attatcttct ttagaatcac
    26401 tacttacaca tgtagacata taataactaa atgtataaaa atctacacaa ccttctttta
    26461 aaatttctaa atctcctttt tccattttta tttcaatatt attttctcca aaatatctat
    26521 tcatataagt tggatattcg ccacgcactt gtacatctcc acagaaataa ttcattactc
    26581 tactatctct ttgagtttta actgcatcat ctggattaca agtataagga tacattgttg
    26641 caaatatttg catacatcca acttgataat ttgaatctat ttcatgagct aacttaacag
    26701 ccttagcact tgctacaaat tgatgatgta acccttgaaa acgaagttga ggtatatcta
    26761 cttggtttat aaaatctgta gtcccttcat ttaatattcc ttgtgataag tagcctccca
    26821 taggcattgt agctgaattt atttcattaa atgtcagcca atattttact tttcctttat
    26881 atcttttaaa tattgtttca cagtaattca tatacaagtc tattacatct cttgaaaccc
    26941 atccattgta tttctctgtt attgcaaatg gtatttcata gtgagatatt gttattaatg
    27001 gttctatatt atattttaaa cattcatcta tcacattttc ataaaattta agtcctgctt
    27061 catttggttc tttctccatt ccagttggaa atattctagt ccatgctatt gaaaatctaa
    27121 ataccttaaa tcccatttct gcaaataaag ctatatcttc tttgtatctg tgataaaaat
    27181 ctattgcttc atgacttgga taaaatgttc ctttttctat aactctcgtt attcttttac
    27241 ttactttttg agaacctcca gtacacatat ctgaacaaga aggacctttc ccatctaaat
    27301 tccatgctcc ttcacattga tttgctgctg ttgctccacc ccataaaaaa tcttttttaa
    27361 atgacatacc tatatcctcc tattataatt cttcaccatt tgattctatt acttttttat
    27421 accaggcaaa actcttcttt ttactgcgtt ttaaagttcc atttccattg ttatctttat
    27481 ccacatatat aaatccatag cgcttcttca tttctccagt acttgcactt actaagtcaa
    27541 tacatcccca tggtgtgtac ccccataaat caacaccatc taactcaaca gcatctatca
    27601 tggcttttat atgagcacgt aagtaatcaa ttctataatc atcatttata tttccatttt
    27661 catctggatt atctactgct cctagtccat tttcaactat aaataatggt ttttgataac
    27721 ggtcatataa agagttcata gttattctaa gccctagtgg gtctatttgc catccccact
    27781 cacttgcatc tagatatgga tttttaagac ttgcaaaaac atttccttct gttaaaatat
    27841 ttacttttgg gtctgcactt gtaagtcttg atgagtaata tgagaacgaa ataaaatcta
    27901 ctgtgttctc tttaagtatc tttttatcat cttcttccat cactatattt attccttttc
    27961 tctccaattc ttttaatgca tatgttggat attctccacg agattgtaca tctataaaaa
    28021 agtaattttc cctatctttt tctatagatt tccatacatc tagtgggtta caagtattag
    28081 gataagtatt tcctgctgct aacatacacc ctattttaaa ttcttgattt atgttatgtg
    28141 ctagtttagt aactaacgca ctcgcaacta attgatgatg cgcagcttgg tattttattt
    28201 gttcttcatt ttctccctct tcaatataga gacctgcacc tataaatggt aaatgaagta
    28261 tcatgtttat ttcattaaat gttagccaat actttacctt acctttaaac cttgtaaaaa
    28321 taacttcaca gtattttaca tataagtcta ctagcttacg atttctccat gctccatatt
    28381 tttttaccaa atacatggga acatcaaaat gacagattgt tactactggt tctattccat
    28441 actttaaaca ctcatcaaat aaatcttcat agaattttag tccctcttca tttggaacat
    28501 cctcaactcc tgttggaaat atcctagtcc atgctattga catacgatag catttaaatc
    28561 ccatctcagc aaataatttt atatcttctt tataattgtg atacatatct attgcttcat
    28621 gagctgggta atagtgttca tcatcacatt caaacatttt cattttccca gccaaaactg
    28681 gaaatctatc ttttccttgc ggtgtaacgt caacattagc tagacctcta ccatcttctt
    28741 tatatccacc ttcacattga tttgctgctg ttgctccacc ccataaaaaa tcttttctaa
    28801 gtgccataat tctacctcca ataatttatt tatagtaact ttattttaaa ataaaaatac
    28861 atatatataa ttcttaatta atagaactct cttttacgct aaaacagtta tcaattcctg
    28921 actattattt acatgctttt gtgtattttc tacaatatcc acataatcag aataattagt
    28981 aacaactact ggtgtttcta agcaatatcc ttcttttgaa atactttcta tgtcaaaagt
    29041 tattaatgtc tgaccttttt taatcttatc cccttgatta acaaaagatt tgaaatattt
    29101 accttccaac tgaatagtat ttaaccctat atgtatcaat agttcacaac cattatcaga
    29161 tataattcct attgcatgtt ttgttggaaa taaagtcatt acagtcccat caaatggtgc
    29221 cactactttt ccttctttag gtgttattgc tacacctttt cccaatatac ctgttgaaaa
    29281 cgctgcatct tgtatctgct ccaaagcctt tacttcacct tttattgggc tatatactac
    29341 ttctcttttg tttttttcta taacgccttg cctattatca tctttagaaa tttctactat
    29401 agaatcatct ttccaaaaaa ataatgttaa aacaaatcct actacaatag ctactattat
    29461 tgctattata gttgcataaa cacctttcat atctccagtt gttggattta tataattagg
    29521 tataccaaat attccaagtc cacccattac ataacttttt acattcatta tccccattac
    29581 agctcctgcc acagcaccac ctacacaact atatatgaat ggtgtttttc ttggtaaagt
    29641 tattccatat attgcaggct ctgttacacc acatatccct gaaataactg caggtataca
    29701 cagctctttt atcttcttat cttttaattt aaagtacatt gctgctacta ctgctgtttg
    29761 tgcaaaagat ggtacaaatg ctcccactaa tattgtgtca aaccccatag tcataaggtt
    29821 tgacatagct attggtacta atccccaatg tagaccaaat attacaagca cttgccacaa
    29881 taaaccagtt aatgcaccac ataaaattgg tgaaaagtta aataaagcta aaaatccatt
    29941 tccaactata ttagaagcat aggtagctat aggccctata accataaatc ccaatggtaa
    30001 agaaactaat aaaactgcca ttggtacaaa gaaattcttt actacatctg gtattatttt
    30061 agcaaacact ttctccaact tagctgctaa taatactatt aaaattactg gtactactgt
    30121 tgaagtgtag ttcattgcta ttactggtaa tcctaagaaa gttatatata ctggtgattc
    30181 aaatattgtt ccagtaaata gattgtataa aggttgacct gtagatgcta gagtatctaa
    30241 ttgaatacct gaatagcata aaactgcacc tataagcatt ccaataaatg gttttaattt
    30301 gaatttctta gcagctgtat atccaagaat tattggcata tactggaaca ttgcatctcc
    30361 aattgcattt aacataatat atgcaccatc agtttctcca tacaaaccca atgctgtaaa
    30421 tactgcatta aatcctttta tcataccagc agcacacaga actcctaata ttggttgaaa
    30481 aactcctgat attatgtcta taaacttatt aagtaatcct tttggcgcat ctgagtctga
    30541 attatctatt gtatctcctc ctaatccagc caaatcgcac acaacttcat aaacatctgg
    30601 aacatgatta ccaataacta cttggtattg acctgcactt ttcattacag taactatacc
    30661 atccattttt tttagcacat catcatttgc tattgactca tcttttaatt taaaacgtag
    30721 cctcgttata cagtgagata aactgctaat attatccttt cctcctacat tcgcaatgat
    30781 ttcttttgat agttgttcat actttctcat tttttctcct ccattttctt tttgaaatat
    30841 gaaggtttgg ccaacttaat gtcaccaccc aatatatata aacctaatta ttaggattta
    30901 tatctgactt aatactttta cttaccactc tttcaacgtg aatagttaag tataattttt
    30961 cctcatctgt taaatcgtag ttgtattttt tatgtataaa ttcatccaac ttttctatgc
    31021 atttataagc atttttatat ttaactttta ttacatttaa taaatcatct tcattatttt
    31081 catgtaaatt attactaaca attcgttctg caaaatactt taagtgagta ataaatctat
    31141 gataataaac tgaatcttca tcaaatacta ttctgtaatg gtatttaacc atatttgaaa
    31201 tttcttgtat tatttgtgta atctcatata tagtttttat actttgatcc atttgtgcat
    31261 taactatatg cagtgctata aatccagctt catcttctga tagtctaatt tcaaattttt
    31321 tttctatata atccaatgcc tctaacccta ttttaaattc actcttataa aatcttttta
    31381 tatcccaaag taatacattt tttacactta ctccttcacg agctctttct attgcagtat
    31441 acatatggtc tgttaaagaa atataaatac tatcatttaa tcttttccct agtttttctt
    31501 tagcatataa aattatttca tctgataatt ccatatatct tataggaata tctgctatta
    31561 gttctttaaa tttatcagat atatttgggt ctgaaagttt gaatatctta tcaattttat
    31621 cctttgatat atgctctcct gtacgttttt tataagctaa acctctcccc ataactacta
    31681 tttccttatg attttcatca gttgtaataa ttacattatt attgagtatt ttttcaataa
    31741 tcattgtgat gcccccttta ttagcattaa gttaaaataa atctttttta attatttaga
    31801 atatttaaaa tgtaatttat tacataaaaa aaaacctatt tattacacat taaatagact
    31861 cccatgagtc ctgagtaata aataggttta gcttgtattt aaacaatcac tatccgtttg
    31921 tattttattt taattattat ttattatatg ttagcaaatt tattttttag tgtcaacttt
    31981 ttctcaatat tttaatttta aataaattat ataaattgaa tcttcaaagt actattttta
    32041 tcaataaaca aaataaaaaa ggtattactt tctttaagta atacccattt atttttaatt
    32101 taagtggttt ttcttaatct catcaagtag tccatcttct aaataaattt ctaatgcaca
    32161 tcctaccatt gcctttactg ctttgtgaag tttatcataa gcttcctcag aatttgcata
    32221 atttagaaat tctttttcat gaacatatac cattggactt atatctaagg ctatttctgt
    32281 atacatagta gggcaaactt gacttacatt acctatatct gtagaaccta ctggaccatc
    32341 tttgccttct aatatatctg ttatacctgc ctcaatcaaa ttattctttg ttatcttttg
    32401 aagtactttg ttatttatta tgttgtcaaa agaattttca aagtatctat aagaaagctt
    32461 agctcctgtc attaactctg caccttttgc acagtttata acttttttag ttacttcatc
    32521 taaataactt ctttcagcag ctcttacgaa aaacttacag gctgcttttt ctggaactat
    32581 atttggagcc tctcctccat ttgaaactat accatgtatt ctaacatctg attttacatg
    32641 ttgtcttagt gcatttatcc cagcaaaagt aagttgtact gcatctagtg cattaactcc
    32701 catatgaggg tgactagctg catgtgctga taccccttca aaactaaact ctaatgaatc
    32761 tattgccaaa gccttacagt ttatattatt aaaagcttct atatgcatct gataacttac
    32821 atcaacatca tcaaaagctc ctgccttaac taaatcacat tttcctcctg ttgtttcttc
    32881 agctggtgtt cctattagta caatttttcc tttaaaattt tcttttaacc tacttaatac
    32941 tatacaagct cctagtgtag atgctgcaat ccagttatgt ccacaagcat gagctggttc
    33001 tttattttct ccatatccag gaagtgcatc atactcagct aaaaatgcta tggtaggtcc
    33061 atcattatct cctaattcag ctctaaaagc agtctccata ttacaatatg gataaactgt
    33121 atcaaatcca tattccttca ttttttctac taagtatttt gatgatatgt attcttcttc
    33181 ccctaattca ggattgttaa atataaaatt acaaacttct tcaatttctt ttccaagttc
    33241 attctctatc tcataagctc tcttctttat actctctatc ataatttacc tcctactaga
    33301 atcctagata taatcctatt gtcaaaaatg ccattacaag aattgttaat actaccatta
    33361 aaggaattac gaatctaacc cactttgacc aaggaatatt ggctactgct agtgctccca
    33421 ttaaaacacc tgatgtaggt gttatcaagt tggttacacc tataccaaat tgataagctg
    33481 taactatcat ttgtcttgat acatctacta agtcgccaag tggtgcaagt acaggtattg
    33541 taagtgatgc aagccctgat gctgatggta ttaaaaatgc tattaatcct tctattaaga
    33601 aagttaaatt tataaatata gtttttggta atccatttaa taaatttgct gcagaattta
    33661 aaattgtatc tattataaat ccattttctg ctattattac gattccacgt gcaaatgcta
    33721 tacacaatcc tgctgtaatt aaatctcctg ctccacttat aaatgaatta actatctcat
    33781 cttgtttaag ccctgatgct attcctgata ttatacctat agctgtaaat atcattgcaa
    33841 tttcagttat gtaccattct agtcttaaaa ctccatatat cataatcgcc atacctattg
    33901 tgaaaatggc tattaccatg gcttctcttt ttgtaaattc ctttatattt gtatcactat
    33961 taaccattaa ttcttgattt agtgatatat catgtactaa agatttttta gggtcttttt
    34021 taactttatt ggcatacatc ataacaaatc ctatagatat agccatatat attataaaca
    34081 taacaactct aaactctatc ccagaacctg gtactatttg tgataatgct tgtgctattc
    34141 ctacagagaa aggattcgta gtagctgctc caaatccagc tgttgctcct ataaatactg
    34201 tggccactgc tgtcaatgaa tcatatccca tactggtcat aagtggaata aatatcatat
    34261 aaaaaggtag tgtctcttca ctcattccaa acgtcgtccc acctagtcca aatagaatca
    34321 ttgaaatcgg tattattaaa aattgaaact tcttcatttt atttacagta tgagctataa
    34381 cagcttctat agctcctgtt ttattaacta tcccaaaagc tcctccaact attaaaacaa
    34441 atccaactac ttcagctgca tctataaatc cgtttatagg tgctgtaaat acatcagtaa
    34501 ttccttgtgg atttgatggt gctttttcat aagttccagc tacaacaact gaccttccgt
    34561 ctatatctac tctttcaaaa tttccacttg gcactatcca agtaagaatt gccattaaca
    34621 ctattagcaa aaatattatt gtaagtgtct gtggcatagt aaactttttc ttttttaaac
    34681 tgctcatttt taatctcccc actttttaaa ttaaatattt tattattgca tccctttatc
    34741 ctattttgta tagatgtaaa aaagctcaac atggatacat gttgagctaa atgtgctaaa
    34801 tacaacatac ctgcatcttc cctttatctt gtttaagata gctctccatc aaaattcggg
    34861 ataattttga tgacagtact gtacctatta aatacagtcc cagcactagc ttcggacact
    34921 agcacttcgg cacttactcc ttttatattg cctcattggt ttccaacctc ttcaatatta
    34981 atcttagtaa atgcaacctc tacctcaatc tgtttgttag atgaggtaat tcttattaat
    35041 ttttattaat tcaaagtaat tgtagcataa tatgtcgaga ttgtaaatat tttcagtata
    35101 ataattctta ctttatgatt tcattccatt tttcacaaat aattttttct ccatataatt
    35161 taaatactgt agtatatttt aaatacacac aaactttcca caaattttaa attattataa
    35221 tacctttata ataaaaaata aaagctatgt aaacttatca atttacatag ctttatatac
    35281 attaatctac tccaagtaca gctttaagct ttgatgctat tgtaataact cgtggaccaa
    35341 atataacttg aactgagttt ttctttttaa caactccact tgcacccaat tcatttaccc
    35401 agaagccatc ttcatagaat ccatcaattg aatttagctc taccctaagt ctagttgcac
    35461 agtttgtaac attttttata ttattacttc ctcctaaacc ttctattata gaatgtacaa
    35521 gctttgtatc tttatcatct acttcctctt ctactgcatt gttgtatgct gttttaccag
    35581 catctacaga ttgtctttct ctatattctt tcttactata aagcttaact tcttctgaac
    35641 ttcttcctgg tgtttttata tcaaacttct tgattgccca tgtaaatatt atataagtaa
    35701 ctataaaaaa tattggtatc aaaaataata atggtaacgc atccactttt tgtggttgta
    35761 ataaattagg aaacatattt ctaaatgatt ctcccattat agaaacatta aacatttctg
    35821 taaatacata cgtcaatcct gccagtggta catatgctac aaaatataat aatggtgcaa
    35881 caaataggaa tgtatactct attggttccg ttacccctac caacatggct gttactattg
    35941 ctggtatcaa tatacttatt gcttttttct tattagcatt atcagccaac ttatacatag
    36001 ctaaagctgc tgctggtaat attgcaaaat gtattaataa acgacctgtt gtgaagttac
    36061 gagttatata agatagtgta tctgggtctc ccatttgagc taatcttata ttattaacac
    36121 cctcgtaaat tgctccacct acttccattg ttccacctac tcttgtatat tctataggga
    36181 atgcaattaa gtgatgtata ccaaatggta ataatgcttt atccattgct ccaaaagtaa
    36241 atgttcctaa taatccacta gaacctatcc aattaccaag tttagataat gccaacccta
    36301 ctggtggcca tataaagtac attgctgtac ccaatactat tgatgctata aacatcataa
    36361 tcattgaaaa tcttggacct gagaaaaaag ataatgcact aggaagttct atgtcaacaa
    36421 atttgttatg tactgcacta gctacaaatc cagctattat agctccaaat atactacaat
    36481 catatgaaaa gattcctaag aaagaaccat ataaagaact tcgactatat gcttctgcct
    36541 ctcctaatcc agatgccata aacgcttctg ctgtaactgt atctgaattt ataccatttg
    36601 ctgctaacaa tgtacttata actgtgtgca ttcctataaa taaaacaatt cctcctaatg
    36661 caccccatcc tttttccttt ttagataatc caaaggctat acctgtaaca aagaatattg
    36721 gtaagtatct cattaccata aatcctaaat cgtttattac tttaaagaat acatttataa
    36781 atgacccttc ttttattagt ccgctaagcc ctaatgcaga aacgttttcc atatttgcaa
    36841 aagctgaccc taatcctata aatactcctg ctacaacaag taatataaca ggtatcatca
    36901 ttgctccaga aagtacttgt aatttatcct tcatagtatt atttcctcca tttttattga
    36961 aatgccaggc tatataatgc atatatatat acttctattg ctttgtataa tgtatctata
    37021 cttatatttt catttgcctg atgtgaattt ggattatccc cctctattgt gggtccaaat
    37081 gcaactccat tttttaacac tcttgcataa gatgcagctc cttttgtaaa aagttcggct
    37141 tcttttccaa aaccttgttt atatgcattt gataatttat ttattaattc actatctggt
    37201 tttacataca gtaattttaa atccttatat atatcaacta ttaaattttc tttttttcca
    37261 atttcattaa ttctattagt tataaactcc caacttatac ccttaggata tctaaagtct
    37321 attttcatat tcaattcatt tttctctaag aagatactca ttatacatat tgtagaaact
    37381 cccatatcta catcttcttt atatatccct aatttttttc catgattatc atctgtaaag
    37441 tatgaattta aaatatccct tattataacg cctttatcat ttagtttttt aattttctct
    37501 aaatcttttg ctaaactaaa tgcacagtta tatgacctgt gtggatttct cgataaagcc
    37561 ctttctcctg tatatcttac taatacttta ccttcttcta gttcttctat ctctgtataa
    37621 taagctaatg attctactaa atctttctta tcattagttt taaaaacagc ctcaattttg
    37681 tcacatacaa atccatcttc tttgtttgac tttatatcaa ctaaattgtg atttctaaat
    37741 ttatctttat ctattttctt tcttagattg aagtataaaa tacctttttc tctatttact
    37801 attgggaagt ttccatctgg agaaaatgca aattttggct gttcattgac actaaagtaa
    37861 tgctccatac attcccaagt cgtctcttca gctccaccca ctatcaatct aatctttctc
    37921 ttcggctttt cattaagttc tcttaagaaa agtaatgcat ataaaatccc tatcaaaggt
    37981 cctttgttgt cattaactcc tctaccataa agatagttat ctttttgaca tactttaaaa
    38041 ggtttactct tccaaagttc tttattatat attggcacca catctatatg attaagcacc
    38101 cctactactt cttctccttc tccatattct atatgaattg catatccatc aaagtctttt
    38161 acaacaaaat ctttgtcttt tgctatttga ataagcttat cgaaggcatt tcttatttct
    38221 atcccaaatg gttgatttat atctgttgta ctttcatctc taattgaagg tatttcaact
    38281 aaacttctaa tatcatttat gatattttct tgattggatt ttaaataatc aataattagt
    38341 tctttgttta ctatcatctt aattatttat caccccttta tattaatttt acaaagcatt
    38401 tcagttcata tctacattat aaattttgaa tattattttt tcaatatttc aaatacttct
    38461 ctaagttacc ataacgatac ggtaaacaat caacagatta tgacaaaatc atactatacc
    38521 tattactaat attttaactt atctattacg tcttttttaa tgtaagttta ttccaaaaat
    38581 acataaaaaa agatgtagcc ttaactacat cttcttatat ctattcaact tccattatta
    38641 tgctattaat atctacattt ccaggttcta tcctatcttt tactgttctc ttaacagaat
    38701 cagcatcatc cattataact acaggagtta ttatatttaa atcatttgac gttataaaat
    38761 ctaaatctac ttccattatt aaatctttag cttttacctt atctcctgcc tttaccttaa
    38821 catcaaacgg ctttccttct agtgacattg tctctaaacc tacatgtatt aatatatttg
    38881 tattatcatt actcttaatc attattgcat gctttgtatc aaatacattg actatttcac
    38941 catcaacagg agaatataac ttaccttcac ttggtattat tgctactcca tctcccatca
    39001 tcttttgggc aaaaactgca tctggaacat cttctaagtt cactaaattt cccttaaaag
    39061 ggctatatat tttctcaatc ttattttctt ttttcttaaa tatattaaac ataacacact
    39121 ctccacatta taaaatttta tgataatatt tttcttaaag cttttgctac accatcattt
    39181 acattagtat cagttacaaa atctgccata gattttactt catctgtagc atttcccata
    39241 gcaataccaa gacctgcaaa tttaatcatg gatatatcat tttcattatc acctatacaa
    39301 ataacttctt cgctatctat acctaatata tcacaaaatt ttttaactgc aatacctttt
    39361 gaagtacctt tgctcataac ttcaaaattt atctttccag aacttactac ttctaaatct
    39421 tcttgttttt taaattcttc ctttgcttct ctcaaagaat ctacattttc tgagaaaagt
    39481 atacatttta aaatttcatt atcagctttt tctaataaag tttcacaact tgcattttct
    39541 attatttcaa ttctgtcttc ttctttattt ttactattta ttaatctata tggatgttct
    39601 tcacctattt cattgttgga tataactatt ttacagcctt taaaatgttt gtttaaatta
    39661 tacttttcac ctattgtata gattttcttt agagcttctt tgctcaaaac cttcttatac
    39721 tcatatccca gtaatttaaa atatgtccca ttagttgata taatatatat atcatcccct
    39781 aaaacacttg taaaatattt catagcatta taaggtcttc ccgtaactaa ggctaccttg
    39841 ataccctttt ccatagcttc ttttagtgct ttaatatttt cttctgatac ttcaaatcct
    39901 ttacccatca aagtaccatc catatcagta caaattaatt tatatcgcat ttgtatcaca
    39961 tcctagtaag atttttctaa tatttcaaca aggtcttctt tctttccaaa aggtaaaaat
    40021 cctaaaactg gacagcttgt tatttgttct aattgttcct ttgttacatt aaactcagac
    40081 atttttgtct taagtcccac gctctttaaa aaactttcta tttcatttcc taaagtttta
    40141 gctgcttcat catcttccac tttttctaat tcttggttta gcattctcgc tactttggca
    40201 aatttctcta tattatcctt atagaacttt tctgcaaatt ttgggaacac tatagccaaa
    40261 gcctcaccat gaggaatatg tgtaactcct cctattattt cacttagcgg atgtggtgct
    40321 gctgctccag catttgccag tgaacttcct cctaatgtat ctgctactgc taactttgtt
    40381 ctgtattcta tattattttt atcttccatt actttaggta gatagttaat gactaattcc
    40441 atagattttt cagacattat ctctgtaaaa tcattagcat ttggatttat ataactttca
    40501 aatgcatgag taaaagcatc aaatgctgtt gatgcagtaa ttctctctgg cagagttaac
    40561 ataagctctg ggtcaactat acattctttt gagaaacaat tttgatgaaa tattgtattt
    40621 ttctcgcttc ctctcgatat tactgctgct tgtgtaactt ggcttcctgt tccagaagta
    40681 gttgatacag ctattaatgg taattctttt tcacttacct tatcgtatat tttaaatgga
    40741 tttgtatatg tactaaacat ataatcccaa tctatctttt ctaaaccaaa agtcaatgct
    40801 aatacttttg cagtatctat acttgaacct cctccaacag ctaaaacaac atctacagat
    40861 tcttttttag ctaattcaaa cccttcattt attaattcta cagttgggtt tggctctact
    40921 ctatcaaagt gtacaacttc tatacctttt tctattaaat tcaatgtaac tcttttatat
    40981 aacttgtcta atggctctac atttggagta gtaactaaca aacatttctt tccatatttt
    41041 cctattattc taggtaattc acttaaagtt ccttgtccaa aatttattct tgatggttga
    41101 taaaagttaa tcatattttt cctcccttaa attattgcct tcatattcaa attatacata
    41161 tcataggatt tttaaggcaa taccttcact gttaactctt atttactaaa gcttatggca
    41221 attattagac aataaatgtt ttattattac catatcatta tgtaaatttt attaagtacc
    41281 catgttataa ttttataatc aatcttattg tataattgac ttaataataa aaatagagta
    41341 tatacacttt tttatgacaa atagcttaaa ggagaattaa attatgaaat atttgataga
    41401 cagtgctaat cttgatgaaa ttcaaaagtg tttaaagtat ggatttaagg gaataaccgc
    41461 taatccatct atgtatctta aagagaaagt aaacttccat gaatttattg agacatgtgc
    41521 taatttaaat ccagaaatat taactgctga agtcattgga aataatttat acgaattaat
    41581 taattcatgt gataagatat taaatataaa taaaaacact ataataaaaa taaatttctc
    41641 tgaagttggc ttagaactta taaacatatt aaatagtaaa ggtgtaaaaa ctgcatgtac
    41701 cttgattttc aatgtaaatc aagcatcatt agctataaat gcaggtgctg attacctatt
    41761 cccttttata ggtagaaatg atgaaaatgg gaatgatggt ataaaaaacc ttacagaaat
    41821 atgtaacctt atatcaataa atggatataa tactaaagtt gttgcagctt ctattaaaaa
    41881 tgtattccac ttaactcaag catctctttg tggatgtgat tatgcagctg taacatatga
    41941 tttatttaaa aaagcttctt ttaaccagct tacagtagat ggtgccaata cttttgaaag
    42001 agattggtcc aaggttaata aatagtaact aagatttagg agggtttgca ttgaacaaaa
    42061 gattattaga tattgctgaa ttattactca acagctcaga ctatattact gtagatacta
    42121 ttgccgaaga attaaaagtt agtaataaaa ctattcgtaa tgaccttgtt attttagatg
    42181 aatggctttt agagtttaat ttatccctag ataaaaaaac tggttcaggg gttattattc
    42241 taggtaatga agatataaaa cttaaagtta ttagagatat caatgataaa tcaaattgta
    42301 tatatgcata ctctcctgaa gatagaaaaa ggtatattct aagtaaatta tttattaaaa
    42361 attctaagtt taggattcgt gatatttgca atgaactaca tgttagtaga gctactgtgc
    42421 ataaagactt ggtagttatt caaaattttt tgcaaaactt taaagtgact ctagttagaa
    42481 aaaccaataa tggagtatat ttagagggaa aagagaaaga tattcgaaaa gcaatttttg
    42541 aacttgctac tgttaataaa agttatactg aacttaaaga aatattattc tcgaaatcta
    42601 atgaatgcaa agatacccca tcaacaaaaa tatttaagga actatttaac tatgattttg
    42661 agaaattatc tcaaatcact ctacacacct tacaagtaga aaaaagtaac cttagtgatg
    42721 agtattatat aaacttctta atacatatag ctatatgtat taaaagaatt tcaattaaaa
    42781 gatatattaa cttatctgaa ggttttttta gagaattatt agaacatgaa tactttgaaa
    42841 aatcaaaaat tttatgtgct aacctaagtg aagcttttga cgtagaattt attgatgaag
    42901 aggtctgtta tatccttctt catatagaag gattacttaa atcaataaag aaaactacta
    42961 ctcttgaacc tcttaaaaat gaagaaatta cagagtataa tctatctgaa gcaatagctg
    43021 ttcattgggg aagtatacta aacttaaact taaaggatga tttaatatta ataaagtctc
    43081 ttgctaatca ccttaaatca gttctacaca gaataaacta tgggtttact attacaaatc
    43141 ccatattaaa tgatataaag agaacatttc cttatactta taaggctgct aaagagtcta
    43201 atactgttat caaacagttg atgaattatg aagtcaatga tgacgaaata ggatatcttg
    43261 ctttatattt aatatcagca atagatagaa gcaaatctcc cctaaatact attttaatat
    43321 gtcactgtag tgatggtgta tctaatttat tagtccaaaa gttaagtttt gaatttaatc
    43381 agattaatat agttaaatct atacctctat cttctatttc atttacgaac tttgatgatg
    43441 tggacttaat actaaccact gctcctgtag actttgaaca taatgctgaa ctgataaata
    43501 taaatgctct tttaagtaag aatgatattt ctagacttag tacaattata aaaaaactat
    43561 attctgaaaa aaataaaata ttatcttata aataaacaaa aataggcttg tctcaacata
    43621 gagataaacc tatttttcat tgtccaaatg ataaaaatat atttgatttc atagaaaaag
    43681 tgcctcatga actaaaaaat tcattttgag acaccttcat tttttatatt taatcttttt
    43741 atttaagctt ttatattttt taaactttta tatttaaagt aaattttatt atttttattg
    43801 agcacaagca ttatttatat tattaatttc ttcagtattc ttaccaaact ttgtagttac
    43861 aaatcctatt gtttttatta tgagtaataa ggatatcaat gaatatatag atatcataaa
    43921 attcttactt attattccag acgctactat tataaatcca tctagcataa ataatataac
    43981 tggcaacttc agaaacttaa ttaccttatt aataagcatt gcaactaaat cagttccacc
    44041 agtactacag ccccttatta ttgctatccc tatagcagtt cctaccaata acccacctat
    44101 cactcctgct actaatagat tgtcagttat gtgagtttgt ggtattactt ttaagaataa
    44161 tggacccata agagttatat ataatgtttt tattccaaaa cctttcccta aaaataataa
    44221 tcccattact aaaagtggca tagatattcc taatgatata ttctcaacag gcattgataa
    44281 gaatgtgcta agtacgactg ataatcctgt tatccctcct ggagctaatg tatgttctgc
    44341 aaagaaaaaa ttaactgcta atgccattaa aaatgctgat attgttatca gtaaataatc
    44401 ttttatcatt tcttttttca tttaataacc tcctatgatt atattgtttc agtataaaaa
    44461 atcaattaaa ttagtctagc ttcctgtagt ttttcataaa attaatgctt ccttaaaaca
    44521 ttaagaaagc atctctttta tcatagttta ttatcttatt ctaaaccaaa ctttgtagct
    44581 gcttcatcta tagcttttct acaattctta acacctgctg ttataccatc tttatccttg
    44641 aatattgctg aagataatat tacaacgtct ggattacatt ctattaatgg ctccatattg
    44701 tctgctctta gtccaccatc tattgctaat tcacacttag gattcttttc gtctatcatt
    44761 tttctagctc tttttactaa atcaacagca ctctttctcc atccccagtt atcttttcca
    44821 tcagtttcat caacaccatg aacgactatt tgcaatctat ctatatcata tatagcttca
    44881 tcaacaaaac ataatggtgt gtaacatcca attgttaatc ctactttcat accaaattct
    44941 ctacagtaat ttattatgta tgcaagagga gcacctaaaa aatgttctgc tggtattatc
    45001 aacaaattac atcctgctgc tgctattttt tctataaata atctatcaca atctcttgtg
    45061 tatatatggc attctattgg tttatctgtc accggtctta ttgcttctat tatttgatga
    45121 ccacccataa gttgcatatt ttttaagtca tgcatatctg ctgcatctga atgtacgtag
    45181 tctacacctg catccattgc ttctttaact acagctgata tatgaccgta atttacatgt
    45241 gctaatcctg ctgctatttt tatgtgtttc atatttgtat acctccatat tttactttaa
    45301 ttagtattaa atattagttt ttaatatttt tttaatattt ttttaatatt ttttaatcca
    45361 gtattacttt tatttagcat tacttttatt gctgtatata tttcttttat gaacttattc
    45421 tgcttttgtt aactgtgaac ttattttgct ttgtaaatta atattacact gatatatttt
    45481 ttttcacaac actttgacta ctccttaaaa gtacctttat attgtggtaa attatagaca
    45541 ttaattgtat ataaaaagtt agttttttac cacattctta tggcatttat tgtctatact
    45601 aactcaattg atatagctag ttcatttaat atataatcag actataaata taaatttact
    45661 cgataaaaag taccattcat aacaatatta atgaaaggga gatgtaaaat gagaaactgt
    45721 attattgccc aatctggagg ccctacagct gttatcaatg cttctgcagt tggtatttat
    45781 gaaaaaaatt tagaaactaa atactttgat aaagtatatt ttggtatcaa tgggattgaa
    45841 ggtatattaa acaaaaatat aatagattca gactccttaa acaaagataa tgtattaaat
    45901 ttaaagcaaa caccttcgtc tggtcttggt tcttgtagat acaaactggc tgattataaa
    45961 ataaattctt atgattatga tagagttata aatatactag atgaatatga aatagataca
    46021 ttattttata taggtggaaa tgattctatg gacacagtga tgaaattttc tcaatactgc
    46081 aaaaacacta cttgtaaaat aaaatttata gggattccta aaacaataga taatgattta
    46141 ttaggaactg acaactgtcc tggatttggt tctgccgcaa agcttatttc tacactaata
    46201 atggaaacat acttagattt ttctatatac aaaaataatg gtatatttat agtagaaact
    46261 atgggtagag atactggatg gttagcagct tcttcttcac tagctagact taatgatact
    46321 ccacttgttg atttaatata ccttccagaa attgatttct atgaagataa gtttatagaa
    46381 gatgttagaa aaatatttaa agaaaaaaat aaggcttata tagttgcatc agaaggtata
    46441 agggataaat acggcaactt tatatgcgaa caaaaaactt ctgctcatga taaatttggt
    46501 cattcccaat taggtggagt aagcaactat ctaaagaatc ttataatatc atcatcaatt
    46561 acaacaagag taaaaacctt agaacttggt gtaatacaaa gatgtgctat gcatttagct
    46621 tcagatactg atttaaaaca agcagctgaa gttggtgcat atgggataga actatctaaa
    46681 aaaggatata caggagttat ggctatcatt aatagaacat gcaataatcc ttataagtat
    46741 gaagttagtc atactgatat ttctaaaata gctaacaaga caaaatattt tcctaaagag
    46801 tggataaatg aagaatgtaa ttatataact gatgaagccc taaattatct atatccactt
    46861 attcaaggta aaccagatat caaatataaa aatggactac cttatttttc aattatttac
    46921 taggaaatta ttataatttg atatacactc aattctatcg ctcttaaata cctaagtggc
    46981 tgttaaataa aattataaat tgttatatga cagtcactta atcgtttaat tatttaactg
    47041 tttagttttt taatttttta atctttttaa tactaataca atttgttgta ataatatctc
    47101 acacccattt tcattttgga tagattataa ttagttagac aataaatatt ttgtcatgat
    47161 attattatat atagtgatac aaatagaaat acttttatca tggctagaaa aacaaagatt
    47221 acacaaagga tttaaaagtc aaacaacttc aattaaaata aatggttaaa ataacttcta
    47281 aatcaactac ataaacaata aaagaacata aagtagttaa atctacaatt ataaaa
//
LOCUS       DAEMHW010000006        47114 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEMHW010000006 DAEMHW010000000
VERSION     DAEMHW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47114
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.006"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            99..1391
                     /locus_tag="KPK67_000249"
     CDS             99..1391
                     /locus_tag="KPK67_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2457271.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            1496..2098
                     /gene="yedF"
                     /locus_tag="KPK67_000250"
     CDS             1496..2098
                     /gene="yedF"
                     /locus_tag="KPK67_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBG2457272.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            2171..2434
                     /locus_tag="KPK67_000251"
     CDS             2171..2434
                     /locus_tag="KPK67_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBG2457273.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            2449..2634
                     /locus_tag="KPK67_000252"
     CDS             2449..2634
                     /locus_tag="KPK67_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBG2457274.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKRLIKK"
     gene            2737..3933
                     /locus_tag="KPK67_000253"
     CDS             2737..3933
                     /locus_tag="KPK67_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2457275.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            4147..4425
                     /locus_tag="KPK67_000254"
     CDS             4147..4425
                     /locus_tag="KPK67_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBG2457276.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            4462..4896
                     /gene="ssb"
                     /locus_tag="KPK67_000255"
     CDS             4462..4896
                     /gene="ssb"
                     /locus_tag="KPK67_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2457277.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            4915..5142
                     /locus_tag="KPK67_000256"
     CDS             4915..5142
                     /locus_tag="KPK67_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBG2457278.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            5285..5605
                     /locus_tag="KPK67_000257"
     CDS             5285..5605
                     /locus_tag="KPK67_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG2457279.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            5613..6569
                     /locus_tag="KPK67_000258"
     CDS             5613..6569
                     /locus_tag="KPK67_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBG2457280.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            6580..8574
                     /locus_tag="KPK67_000259"
     CDS             6580..8574
                     /locus_tag="KPK67_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBG2457281.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            8575..9024
                     /locus_tag="KPK67_000260"
     CDS             8575..9024
                     /locus_tag="KPK67_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBG2457282.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            9043..10371
                     /gene="dnaB"
                     /locus_tag="KPK67_000261"
     CDS             9043..10371
                     /gene="dnaB"
                     /locus_tag="KPK67_000261"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBG2457283.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            10576..10884
                     /locus_tag="KPK67_000262"
     CDS             10576..10884
                     /locus_tag="KPK67_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457284.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            10966..12255
                     /locus_tag="KPK67_000263"
     CDS             10966..12255
                     /locus_tag="KPK67_000263"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBG2457285.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            12581..12656
                     /locus_tag="KPK67_000264"
     tRNA            12581..12656
                     /locus_tag="KPK67_000264"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12614..12616,aa:Lys,seq:ttt)
     gene            12663..12737
                     /locus_tag="KPK67_000265"
     tRNA            12663..12737
                     /locus_tag="KPK67_000265"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12696..12698,aa:Glu,seq:ttc)
     gene            12742..12817
                     /locus_tag="KPK67_000266"
     tRNA            12742..12817
                     /locus_tag="KPK67_000266"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12775..12777,aa:Val,seq:tac)
     gene            12851..12925
                     /locus_tag="KPK67_000267"
     tRNA            12851..12925
                     /locus_tag="KPK67_000267"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12883..12885,aa:Thr,seq:tgt)
     gene            complement(13308..14300)
                     /locus_tag="KPK67_000268"
     CDS             complement(13308..14300)
                     /locus_tag="KPK67_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2457286.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(14309..15367)
                     /locus_tag="KPK67_000269"
     CDS             complement(14309..15367)
                     /locus_tag="KPK67_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG2457287.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            15492..16955
                     /locus_tag="KPK67_000270"
     CDS             15492..16955
                     /locus_tag="KPK67_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBG2457288.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            17057..17854
                     /locus_tag="KPK67_000271"
     CDS             17057..17854
                     /locus_tag="KPK67_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2457289.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            17867..19969
                     /locus_tag="KPK67_000272"
     CDS             17867..19969
                     /locus_tag="KPK67_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBG2457290.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSEYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            20037..20516
                     /gene="rlmH"
                     /locus_tag="KPK67_000273"
     CDS             20037..20516
                     /gene="rlmH"
                     /locus_tag="KPK67_000273"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBG2457291.1"
                     /translation="MNISIVVVGKIKEKYLKLGINEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSRMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            20665..20976
                     /locus_tag="KPK67_000274"
     CDS             20665..20976
                     /locus_tag="KPK67_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2457292.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            21024..21341
                     /locus_tag="KPK67_000275"
     CDS             21024..21341
                     /locus_tag="KPK67_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2457293.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            21554..22255
                     /locus_tag="KPK67_000276"
     CDS             21554..22255
                     /locus_tag="KPK67_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2457294.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            22480..23781
                     /locus_tag="KPK67_000277"
     CDS             22480..23781
                     /locus_tag="KPK67_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2457295.1"
                     /translation="MEKVASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            23918..25012
                     /locus_tag="KPK67_000278"
     CDS             23918..25012
                     /locus_tag="KPK67_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG2457296.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIMLLDFIEPWSKFKLILEGE"
     gene            25014..26147
                     /locus_tag="KPK67_000279"
     CDS             25014..26147
                     /locus_tag="KPK67_000279"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBG2457297.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKRYKTNVYALINIAKQGITSQDELSNMMNIDEYELRRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIEENE"
     gene            26165..27274
                     /locus_tag="KPK67_000280"
     CDS             26165..27274
                     /locus_tag="KPK67_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBG2457298.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            27339..28079
                     /locus_tag="KPK67_000281"
     CDS             27339..28079
                     /locus_tag="KPK67_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBG2457299.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            28084..29076
                     /locus_tag="KPK67_000282"
     CDS             28084..29076
                     /locus_tag="KPK67_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBG2457300.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSIKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIGIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(29264..30145)
                     /locus_tag="KPK67_000283"
     CDS             complement(29264..30145)
                     /locus_tag="KPK67_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2457301.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            30418..31806
                     /locus_tag="KPK67_000284"
     CDS             30418..31806
                     /locus_tag="KPK67_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457302.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKENPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFTPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            31834..32787
                     /locus_tag="KPK67_000285"
     CDS             31834..32787
                     /locus_tag="KPK67_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG2457303.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIETLTKEKQAGVSLG"
     gene            32847..33071
                     /locus_tag="KPK67_000286"
     CDS             32847..33071
                     /locus_tag="KPK67_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457304.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIGQAREATNYNIGNG"
     gene            33064..33576
                     /locus_tag="KPK67_000287"
     CDS             33064..33576
                     /locus_tag="KPK67_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBG2457305.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTVMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            33608..34462
                     /locus_tag="KPK67_000288"
     CDS             33608..34462
                     /locus_tag="KPK67_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBG2457306.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            34996..35790
                     /locus_tag="KPK67_000289"
     CDS             34996..35790
                     /locus_tag="KPK67_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2457307.1"
                     /translation="MHTIGQVSKFLGISRDTLKFYEGKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            35894..36700
                     /locus_tag="KPK67_000290"
     CDS             35894..36700
                     /locus_tag="KPK67_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2457308.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVVSDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVFIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRYSPLKA
                     SIIVGFWWGIWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            36762..37283
                     /locus_tag="KPK67_000291"
     CDS             36762..37283
                     /locus_tag="KPK67_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG2457309.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            37698..39785
                     /locus_tag="KPK67_000292"
     CDS             37698..39785
                     /locus_tag="KPK67_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2457310.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEEFNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPKLEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            39800..40087
                     /locus_tag="KPK67_000293"
     CDS             39800..40087
                     /locus_tag="KPK67_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2457311.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTELEKFLASKEG"
     gene            40114..41385
                     /locus_tag="KPK67_000294"
     CDS             40114..41385
                     /locus_tag="KPK67_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBG2457312.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            41443..42042
                     /locus_tag="KPK67_000295"
     CDS             41443..42042
                     /locus_tag="KPK67_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2457313.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            42047..42952
                     /gene="dapA"
                     /locus_tag="KPK67_000296"
     CDS             42047..42952
                     /gene="dapA"
                     /locus_tag="KPK67_000296"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG2457314.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            43058..43690
                     /locus_tag="KPK67_000297"
     CDS             43058..43690
                     /locus_tag="KPK67_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBG2457315.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNANKIREIIKNIRKI"
     gene            44168..46675
                     /locus_tag="KPK67_000298"
     CDS             44168..46675
                     /locus_tag="KPK67_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2457316.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKLMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDTYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYITILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAECPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQEYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNDKLNQQIQQYQNILDNINQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
BASE COUNT        18363 a         5109 c         8462 g        15180 t
ORIGIN      
        1 tgtacagtga aaatattgta aaataaaagt agtgttttta aaataaatat aagttataat
       61 tatattataa aaaaatgtct tcaaaaggtg ggaatagtat ggaaattttg agcttaggag
      121 aaaagattaa aaaattaaga aaagaaaaaa acatgacatt aaaagaatta gctggagata
      181 ggataacagc agcacaaata agccatatag agagggataa atctcatact agttatgaac
      241 tgttggaata tctaggcgat aagctagatg taagtgtaga atacttatta gagaccaagg
      301 aaatgcaatc aaaaaagata actgataatt taatactaca gagcgaaatt tatataaaag
      361 aaaatgaatt aaacaaggct gaggaccaaa taaaagaagt tttagatata tgtaagagat
      421 acgatttaat ggagaactat ggcagatgta attttttaat ggctaatatt aatttgaaaa
      481 aatctaatta taatgatgct atagaaaact ttgaaaaggc attgtacttc tttataaaaa
      541 atagtgatag tgaaaatata cttaagtgtt atttaaacat agggaaaata tatatgcagg
      601 aagattttta taaaggtgct ataagccact ttaattttgc agaggaaatt ttgtcagaga
      661 ataaatttga ggatgttaac atacacaagg aactatatag taagatggca tattgttaca
      721 taaaactaga taatccaaat atgtcattga agtacataga aaagataaat gaaatagata
      781 atagaaatga tagaaaagaa gatgtcgata ttttggtgtt aaaagcaaat aatatgcttg
      841 agattggaaa atatgaagaa tcaagagatt actttaaaaa agcactaaaa atacttgaga
      901 gtgaggataa taaaactgga ttagcaaatg tgtatttaac aatttgtgat gtttacagaa
      961 aaataggtga aacagataga gtacttgaat attcacaaaa ggtatatgac ataaagaaaa
     1021 atgatgaaga tgaatacatg atgtcaggat tatttaagat aatagaagca tatatagata
     1081 aagaagatta tgatttagct agaaaatatt gtaagatagc cttagcttca tcaataaaaa
     1141 ataaaaataa attcaatgaa tataaagcat tgagattcta tgctaatatg tataaaaatc
     1201 agaatgaagt aactcttgca attgaatatc tgattaaatg cataaaaata gtatcagatt
     1261 taggcaacaa tagaatattg gccaatttat atatagactt aggtcaatta tattcaaata
     1321 tatcaaaaga aaaagagtta gaatattatc aaaaaggtgt atttatgtac aaaaacttag
     1381 aaataatgta gggtaaataa acctataagg gtatagaaac aatctataac aagcaagata
     1441 atatctatga taaaatataa attgtatgaa tatttttaag gagatgaatc agacaatgag
     1501 tataaaaata gatgcgagag ggcttgcttg tcctaaacca gttataaata caaaaagaga
     1561 attggataac ctagaagaag gtgttgtagt tacaatagtt gataatgaaa cagctaagga
     1621 gaatatatta aaacttgcta aatctttaag ttgtgaagca aatattgttg acgaaaaaga
     1681 gaattttata tctatagaaa ttagaaaagg taaaaatata ttagataaac aaaatgttgt
     1741 agataataaa gaaagtgaat tagacaatac atgtatattt atatcttcag ataagatggg
     1801 acttggaaat gacgaattag gaaaagtgtt aataaaagga tttatatata cattaacaga
     1861 atctaaacct tatcctaagt atgtactttt agttaatgga ggagtaaaat taagtgctga
     1921 aaatgaagaa accatagaaa atctaaaaat acttgagaat atgggtgtag aagttttatc
     1981 ttgtggtact tgtttagatt attataattt aaaagacaaa ttacaagtag gtacagttac
     2041 aaatatgtat actatagttg agacattaaa gaatgcatca aataccattt ctatataatg
     2101 tatgtttgtt taataatata aaactaataa taaagattaa ctcaaataga tagtcagaaa
     2161 ggattaagta atgaatcaaa tgtatatagt gtcatttaat tcgacacatc atgcgataag
     2221 atcagaaaag ttatttggtg aaaatagtct aaaagtaatg gctcttccaa caccaagaga
     2281 aataacagca agttgtggaa tatcaattaa attttctttt gaagatatgg aaaagataaa
     2341 aactattttg gttgaaaata atgtagatat aaaaggaatt tattgtataa gtaaattaga
     2401 aaatggttca aaagaagtag agaagttaga ttaggaggct aattatttat gccaatggat
     2461 ttaaaaatag gtgatattgt tgaattaaaa aagcaacatg catgtgggtg taaagagttt
     2521 gaaatcataa gaacgggaat ggatataaag ataaaatgta ctaaatgttc aaggctaata
     2581 atgcttgata gagagacctt agaaaagaga gtaaaaaggc taataaaaaa gtgataaaaa
     2641 tttaacactt aaaaaatgtc aaataccgta tattaatatc caaaattttg gtataataag
     2701 aattgaagaa aacattttca tgggggtgct ttaattatgg cagttaaata tgcaaaaaga
     2761 atgcaaggat tacaaggatc agaaatacgt gagcttttaa aacttactca acaaccacaa
     2821 ataatatctt ttgctggtgg aatgccggct ccagagttat ttccagttga ggaaatgaaa
     2881 aaagtatcag ttgcagtgct tgaagaaaat ggaagatcag ctatgcaata tactacaaca
     2941 gaaggatatg aaccattaag agaaaaaata gcagctagaa tgaatgataa aaacaaaact
     3001 aatgtaaaca aagatgatat attagttaca agtggttctc aacaaggtct agattttgca
     3061 ggaaaagtat ttattgatga gggtgatgta attttatgtg aaagtccatc ttacatagga
     3121 gctataaacg catttaaatc ttatcaacct aaattcatag atgttccaac agattctgat
     3181 ggaatgataa tggaagaatt agaaaagata ctagaaacaa ctgatagaat aaaaatgatt
     3241 tacgtaatac ctgacttcca aaatccaaca ggaagaactt ggccacttga aagacgtaaa
     3301 aaattcatgg aaatagtaaa taaatttgaa ataccagtaa tagaagataa tccatatgga
     3361 gatttaagat ttgaaggaga gactttacca tctttaaaat caatggatac aaaaggatta
     3421 gtaatattct taggaacttt ctctaaaata ttctgcccag gatacagatt aggatggact
     3481 tgtgcttctc cagaaatttt atctaagttt aactttgcaa aacaaggtgc agacttacaa
     3541 gcatcaacta tatctcaaat ggaagtaagc aaatttatgg atatgtatga cctagatgca
     3601 catgttgaca aaataaaagc agtttatgtt aaacgtagag atgtaatgct taagactatg
     3661 gaagaagagt tcccagaagg attagtattt actcatccag aaggtggatt atttacatgg
     3721 gtagagcttc caagtaattt aaatgctaaa gaattaatgc caaaatgttt agataagaat
     3781 gttgcttatg ttgctggagg aggattcttc cctaatggtg gtagagaaaa tactttcaga
     3841 cttaactatt caaatatgcc agaagaaaaa ataatagaag gtataaaaaa tatagcagct
     3901 gtattaaaag aagctatggg tgtagaagct taattaaaaa taataactta tatataatac
     3961 aagggatggt tgttgccatc ccttgtttgt ttgaaaagta tataattcac cttatactaa
     4021 actttgtcca aaatttaaat tgacagaaat aagaataaat gataaaatgt gtaggtatga
     4081 gattttagtc tcatttctct gctctaagag tgtagagccg ttaagtccaa agggaggtga
     4141 attatagtga gaaattatga attagtttat gtggtaaagc caaactctga tgaagaagta
     4201 agagaggcta tactaaacaa agttaaggaa gttgttgcaa ctgacggaga aatagttaaa
     4261 gttgatactt ggggaactaa aaaattagct tatccaatag ctaagtttac agaaggtttc
     4321 tatgtattag ttaacttcaa atcagcagta gacgttccta aagagataga cagaaactta
     4381 aagataaatg aaaacgtaat aagacatatg atagttgttg cttaataact ggttttatta
     4441 taaaaaatag gtggtgtttt tatgaatcaa gttgtattag tgggaaggtt gactagagac
     4501 cctgaattaa gatacatccc aggtacagga acaccagtgg catcctttac aatagccatt
     4561 gatagagact atgtaaaaaa agatggatct aaagaaactg attttatacc tgttgaagta
     4621 atgggtaaat ctgctgaatt ttgtgctaac tacataacta aaggtagatt agtagcactt
     4681 caaggatcta taagagtaga caattatcag acacaatcag gtgaaaagag aacttttaca
     4741 aaagtaagta ctagatcagt acaagctttg gatagtaaga ataaatcgga aaattcatat
     4801 agagagagtg ctccagcttt tgagccaagc tttgaacctc aaggtttaga cccacaaggt
     4861 ttccaagcta tagatgatga cgatatacca ttttaaaaag gagggaaaaa agtcatgatg
     4921 aataagaaaa gacgtaagaa aaaaagagtt tgtcaatttt gtgctgacaa aaacgctaaa
     4981 atagattaca aaagtactca aagattacaa aagtatataa ctgaaagagg aaagatatta
     5041 ccaagaagaa tatctggaac ttgtgctaag catcaaagag aattaacagt tgctataaaa
     5101 agagcgagaa atatagcact tttaccatat acattagact aaaaaaatga gccttgtgct
     5161 cattttttat tatatgtatt tatatatcta aatgaggcga gctataaagc tcgttttttt
     5221 gtttatatac ttaaatatat tattatttat gatataatat tatatattaa ggagggttgt
     5281 aaacgtgaaa atagataaag gttctgagat aacgagaaac ataaagataa tagaatggat
     5341 gaaaacagaa atattaatga gtgtaagtga tttgtttaat ttattgttta aaggggtaaa
     5401 accattagat gaagcaattc aagatacttt agcaaatata ataatgataa catatctctt
     5461 agcaaagaga ttaggaatta gttttagaga tgtggattat aaagtcaagg aaaaaataaa
     5521 aattggtata gatgaggacc atagcgttga aagatggtat ggagatttat ctaatttaaa
     5581 aaaacatatg gataataggg agtgatatta aattgaataa taaaattaga ctatctaagg
     5641 caatgtctat aatagtttta gctataataa tcgctttagt aatagcctat attccaatgc
     5701 taggaatatt tagtatgtta gcagctgttc catatgtaat tataggtgct attactgata
     5761 gaaagtattc tcttatatct attttggtta cattttgtgt actgattcta tttgcagaca
     5821 tttcttatgc attaaatata tgtataatgt attctttacc aggtatagta ataggaaaga
     5881 tgctaaagag aagctttgaa caagaggaca gtaataaatt cgaaccgata tatggtggaa
     5941 ccataatatt tgtactttca atgcttgtat atttttttgt attaaagaca tttttaaatg
     6001 tgaatctatt agatgaagtt tctaagatga tatcagaaat tgtaaatatt caaaaaaata
     6061 gtttttctgc aactgaactt aaagtgtttg ataaaatgaa gccagatgag attgtaagtt
     6121 attttacaaa tatgctacca atgatgttat ttttacaagg gttgttatca gcgtttgtca
     6181 catattattt gagtgtattt tttataaaaa ggattacaaa gatgaatata agatttccta
     6241 agtttgctga tttttattta ccgggaaatg ctatactcac aactttctta ctttatttat
     6301 tagtcttatt tattcaaatt attggttcaa aattatatac agaattaata atgactaatt
     6361 tacagctagt ttttaattta atgtttgtaa ttcaaggtat agcagtttgc atttatttct
     6421 taaagaaatg gataagacag ggaccaaata aaattatgtt tttttcaggg ataattttat
     6481 gtttatttgg atttatggga atatcttttg ttggtatggt agatagtata attgacttta
     6541 gaaaggtaag aagctataaa tccacttagg aggatagaaa tgagtaataa acaaaccttt
     6601 aaattaaata tgccagaaat aaatttatat ataattgtta ttggtatttc tagtataatt
     6661 ttgctttatt acaacttgta tgtagggtgt ttatttttct gtatttttgt atatatggtt
     6721 tttcacaatt ggagaactac taatattaga cgtcatgaat ggacagagta tattcaaaat
     6781 ttatctttag atatagatga aacaactaaa aaagccataa taaatcttcc aataccatta
     6841 tgtattttag aatttgatgg aaatatatcc tggtataatg gaaagtttta tgatatgatt
     6901 ggtcaaaaag atttacttga taaaaatata gaggatatag ttaaaaatct taatttaaga
     6961 aaagtattaa atgaaaataa agagatgtac acagaaataa actataaaga aaaagaatat
     7021 acaataattt ataatgttat aaaaaatgat caagaaaaaa atcctaaata tttgatgata
     7081 ttatactgga tagacaagac tgaatattta aaagtaaaac aaaattatga tgatgaaaaa
     7141 aatgcaatga tgctaataca agtagatgga tatgatgaag tattaaaaag tgcagcagaa
     7201 gataagagag ctttaattaa tgttgaagta gagaaaatac tttctgcttt agaattaaat
     7261 tcaaatggtg cattgagaag aacttcaaaa gacaaattct tcttggtaat gcataaaaaa
     7321 gagctaaaaa aattagaagc tgagaagttt tctattttag atacaataag acatattgat
     7381 tatggaaata atcttcctgt tactataagt ataggaattg gtatagatgg agatacatta
     7441 aatgagaacc taaaacttgc tacgggtgct ctcgatttag cacttggtag aggtggtgac
     7501 caggctgttg taaaaacaaa agataaattt gtgttttatg gaggaaaatc taaagctgta
     7561 gaaaagaaaa ccaaagtaaa atccagatta ataggtcatg ctttaagaga ggttatacaa
     7621 caaagtgacc aagtttatat aatgggacat aaatatcctg atatggatgc tatgggagca
     7681 gcagttggtg tttatgatat atgtaaatcc tgtaacaaaa ctgcaaacat agttttacaa
     7741 tctgtaaatg aatctataga aatatttata aataaaataa atgaaaataa ttactataaa
     7801 aagttgttta ttggaaaaga ggaagctatt gacaactgca ctaaaaatac attagttgta
     7861 gtagttgata ctcatagacc aaattatact gaatgtgaag aactgttaaa gctatcagag
     7921 aaggtagtag ttatagacca tcatagaaga ggtgtagagt ttataaatga tgcagtgctt
     7981 ttattccatg agatatatgt atcctcaacg tgtgaaatgg ttacagagtt agttcagtat
     8041 atggatgaag atgtaacaat aaataaactt acagctgaag ggcttttagc aggtataagt
     8101 ctagatacta aaaactttgc attcaaaaca ggtgttagaa cttttgaagc tgcttcttac
     8161 cttagaaaag ttggagcaga tactatagag gtaaagaaat tcttcaattc tgatgtaaaa
     8221 gatttcataa taaaagctga aataattcaa agtactaaaa taattaataa tagaatatgt
     8281 ttagcatatt caagtactga gatagatagt ataaatgtta taatagctca aactgctgat
     8341 gagttattaa atatcaaaga agtagaagct tcctttgtat taggtgaaaa agatgatact
     8401 atatttataa gtgcgaggtc tttaggacaa ataaatgtac atgtacttat ggaaaaatta
     8461 ggaggcgggg gacacataga tatagctgga gcacagttaa aaaatgtttc tctaaaagaa
     8521 gcttataaga tggtaaataa aataatagag gagtatttgg aggaggaaga atagatgaaa
     8581 gttatattat taaaagacgt aaaaggaact ggtaaaaagg gagaaatgaa agaagtaagt
     8641 gatggatatg caagaaattt cttatttcct aaaaagatgg ctgtgcaagc agatagtgta
     8701 gcaataaaag aattaaatga aaagaataaa tctaaagaaa taaaagcaca aaaagagtat
     8761 gaagaagcag ttttattagg taagcaaatg gaagaaatta atatagaaat atattcaaaa
     8821 gcaggagaag gtggaagatt atttggttca ataacttcta aagaaattgc tgagcaatta
     8881 aagaaacaaa aagacataga tgtagataaa agaaaaatat tattagatga accaataaga
     8941 tctttaggtt caacttttgt agaaataaaa atacatcaaa aagtaactac taaaataaga
     9001 gtggatgtaa aagaaaaaca gtaataacta gaggtgatta agatggaaga tatgacgaga
     9061 attcctcctc atagtgtaga atcagaacaa tcaatattgg gttctatact tctagataaa
     9121 gatgctataa taacggttac agaaactata aagcctgatg atttctataa agaggctcac
     9181 aaaataatat atgaatgtat gataacttta agtaataaag gtgagcctat agacttgata
     9241 acattaacag aagaactaag aaaacaaggg catcttaatg atattggagg aatcagttat
     9301 attacaagtc tttctactat agtaccgacg acttcaaatg taaaatacta tgctgatata
     9361 gtaaaagaaa agtctgtact tagaaagctt ataaaagctt ctaatgaaat aataaatcta
     9421 ggatatagtg gagcaactaa gattgaagat gttttagaac aagctgaaaa gagtatattt
     9481 gatatatcac aggagaaaac tagtgatgat tttaaatcta taaacttagt tttgatggat
     9541 gcctatgata tgatagaaaa attatacact aacaagagtg atgtcactgg tataactact
     9601 ggttttaaag atttaaacaa aaaaataaat ggtcttcaaa ggactgattt aatattaatt
     9661 gcagctagac cagcaatggg taaaactgcc ttttctttaa acttggttca aaatgcagcc
     9721 ttaaaaggtg atgcctctgt agctgtattt agtttagaga tgtctaagga acaattagta
     9781 caacgtatgt tatcttcaca atctagtgta gaattaaaaa aaataaagac tggaacactt
     9841 aatgataatg attggcctag aattatagat gctatggcag tattatcaga tgccaaaata
     9901 cacatagatg atactcctgg tataaaaata tcagaactaa ggtcaaagtg tagaaagtta
     9961 aaaatagaaa aaggactaga tttagtgctt attgattatc ttcaacttat ggaaggtgaa
    10021 ggtaataatg aaagcagaca acaagagata tctaagatat caaggtcact aaaaatatta
    10081 gcaaaagaat taaactgtcc tgtagtagca ctatctcagt tatcacgtgc accagaacaa
    10141 agagcagacc atagacctat gttatctgat ttaagagagt ctggagcaat agaacaggat
    10201 gcagatatag ttatgttctt atacagggac gaatattatc atgctgattc tgagagtaaa
    10261 aatataggag aagtaataat agcaaaaaat agacacggtg aaactggttc tgtggaactt
    10321 gtttggcttg gtgaagttca aagatttggg gataagttga gagacctata atctaacgaa
    10381 tatccacaga acgtcctttc tttctacagg aaaaatgtgg atacatgtca ctaaatatgt
    10441 ccaaacattg aaatttagct aaaaaactat ccacactatc cacagatagc ctgttgataa
    10501 gtgtgaataa gtagataaag caagcttttg gcttgcttta tctgtgtata aatattaatt
    10561 attaggagga ataatatgga aaaaataagc ttaaaatata tatatcccaa tataattaaa
    10621 gtacttgatg aaataaactt atttagagta attgacaata atttaagaga atctattgtt
    10681 gtttatgcta ataatgtgga taatcagtat catataaaca tgactaatac aaactttgga
    10741 aacataatta acatatgtaa attagaaaaa cttttagatg tggataaatt tatggaaaaa
    10801 gttataaaat atgaaaaaga aataatagag aaagaagagt tttcaaaaat agaagaatat
    10861 atgttaaata ttggagaata ctaaaaaagt tgaaaaaaat acgaatgttt gatagaataa
    10921 gtaagtaaag tgttcgagag tatataaaat atagaggtga attacatgaa aacagttgca
    10981 attgttggtt ctcaatgggg agatgaaggt aaaggtaaag ttatagatta tcttgctact
    11041 caagcagatg tagtagtaag agggcaaggt ggaaataatg caggacatac attagttgta
    11101 gaaggtaaaa aatacgcatt acatctaatt ccatctggag ttttaaatcc aaatacagtg
    11161 aatataatcg gaaatggaat agtgtttgac cctaaaggat ttttagaaga attggaaatg
    11221 tttaaaacgg ataatataag tacagaaaat ataaaaataa gtgatagagc acatgtcata
    11281 ttcccatatc ataaagaact agatgcattg tcagaagaag ccagaggcga tttaaagata
    11341 ggtacaacaa aaaaaggtat tggaccttgt tatatggata aaacagagag atctggtatt
    11401 agaatatgtg acttaatgga taaagataaa tttgctataa aattaaaagc tcaaatagat
    11461 gctaaaaatg aaatagttaa aaatatatat ggaaaagaag aattatttga ttttgaaact
    11521 atatataacg aatatctagg atatgcagaa caaataagaa aatatgtggc agatacgtct
    11581 gttattgtat atgatgcagt aagagcaggt aaaaaagtat tgtttgaagg agcacaaggg
    11641 actttattag atttagattt aggaacttat ccatttgtta catcttctca tccaacatct
    11701 ggtggatttg caattggtgc aggtatagga ccaaatatga taaaagacgt tgttggtata
    11761 gtaaaagcat atactacaag agttggagaa ggaccgtttg ttacagagca aataaatgaa
    11821 actggagata agataagaga acaaggacat gaatttgggg taactactgg tagaccaaga
    11881 agatgtgggt ggtttgatgc agttatagtt aagtatgcag ctagagttaa tggattgact
    11941 agtatttcat ttatgttact tgatgtatta actggatttg ataagataaa agtttgtacg
    12001 tcttataaaa tgggcgataa gataataacg gatttcccag cttcattaga tgatttagca
    12061 aaatgtgaac ctgtatatga agaattagat ggatggaatg aagatataac tcaaatagat
    12121 aactttgatg accttccaga aaatgcaaag aaatatgtag caaaaataga agaactagtt
    12181 ggagtaagtg tagatatggt ttctgttggt ccaaatagag ctcaaactat aataagaaga
    12241 aatatatttg cttaatattt tatcaattaa ataatttaaa atagttgaag aagagtatct
    12301 caagataatg tttatagtta ttagagatgc tcttttatat ttgacttaca ttgatttttt
    12361 attaagttaa agctacttat acatattcta gctgaaatta aagttgaatt tatgtagata
    12421 catataggtt ttttaaaaag aattttgttg aaaataaatg gctaaagatg attttaatta
    12481 tattctaaaa aaagtaaaaa aaactattga ctaaaaatta tatagacgct ataatataac
    12541 ttgtgagtga aagaaacgac gaaaaaagtc gaaaaaatgt ggtctattag ctcagtcggt
    12601 agagcacctg acttttaatc agggtgtccc gcgttcgagt cgcggataga tcaccaaaat
    12661 atggctcgtt ggtcaagagg ttaagacacc gccctttcac ggcggtaaca ggggttcaat
    12721 tcccctacga gtcaccagta tgggactata gctcagctgg gagagcacct gccttacaag
    12781 cagggggtca caggttcgag ccctgttagt cccaccaaat tttgaattag agataaatac
    12841 tagattatat gctggtgtgg ctcaacggta gagcagctga cttgtaatca gcaggttgta
    12901 ggttcgattc ctatcaccag ctccacaaag tacataaatt caaattaata taattttcta
    12961 tgttacagga tacttttaag tatctttttt taatttcatt tttattgtat taaaaatgaa
    13021 attaaacata caacaaaatt ttgaattact tgcaatgtga taattttaaa tataagattt
    13081 agaatcattc tagcatagta ttatgtaata tagcaaattt aatttatagt atcaataaat
    13141 atagactact aagaatgtaa atgatttgtt agattaagat acattagata tgtaaataga
    13201 aaaataaaaa gtatgtttta atttatgtgg cataagtttt aaacagagaa gcaagtttgc
    13261 tcttacctct ctatttaaaa ataaaaaaat gttttttaag ggctatttta tttaaatctt
    13321 tcccatctca aatcatctcc agtaaattct aaaatcctaa acttatctaa gattctagag
    13381 agagttcttt gagtgtaagt atttcctatt tgagatggtg ttaagttggt tgaaataatg
    13441 atttttttac cagcaacaag tcttgtattt acaatattga agatttctcc actagtaaaa
    13501 gaattattaa gttcagttcc aaggtcatct attattaata aatcacaatc aaacaagttt
    13561 ttataattat cttcacttat ttgattattt gtgtctcttc taaatttata atcttcaaga
    13621 atatctaaaa ttctaaaaga agtttgatag attacaacat tgcctttatc taacaattcc
    13681 tttgcaatac aattgcacat ataggtcttt cctagtccag tagaaccata aaacagtaga
    13741 ttttcactat tatccttctt aaaatcatga acaaaatttt cgcagatact taaattattt
    13801 agcatatttt ctctaggaga tatttcacca tcagagcctt tcttaggaga aaatatattc
    13861 aagttaaaat tcgaaaaatt ttcctgactt aaaatcctat ctaaattaga cattttataa
    13921 gcttcattta caatttcttg tttgaggcaa gaacattttt ctccattagg taaaaatcct
    13981 ttatctttac aagaaataca ttgaaacttt atttccaagt aatcaagagg tatgttactt
    14041 tcagctaaaa gtctttcttt ctttactttt aaagactcta tattctcttt agtcttcttt
    14101 acaatttcat ctttagactt gggatttaaa agtactatct tagctaaact gagacctatt
    14161 ttagaaattt catcatctat agattttatc tcagggattc tattatatac ctcatttttt
    14221 ctatgttcta ataagagttc attatcatct cttctcttag catatttagc aagtattttt
    14281 cttattttat cctcattcat ttaatcacct acttaaactt ttctttttga ctttttttaa
    14341 taatctcatc gagctcatca gaggtatact gagtgaatgt ttcgttgaaa ttatgaaact
    14401 tagttttttt gtaagtattg ttgttttgag tagtattaat ctgcttctta ttaatattct
    14461 ccttaactat acgttcttcc tctttttgct ttaaatcatt taaatttttt atattttttt
    14521 cattccaatt tttaattata ccatttatat aagatatact tgggtttgaa atattcttag
    14581 acttagaaca tgcatttatt atcaaatcta tatccatatt aaatttatca aaccaagtgt
    14641 ccataagttc tttttctgat tttgatggtt gtcttgaaaa tcccaattca ttgaaaatcg
    14701 ttttatataa aatatatcgt tcacttctta ccaaaaagct ttcttcaaca tcttttggcg
    14761 tatataaatt tgaatcatac caatttctaa taatgccttc aatgtattta actggctttg
    14821 atgtaccagt tttatctttg acgtattcat atgcgcaaag tatcatatcg gggctcatat
    14881 tgtatttatt cattatatcc ataatactca tcttttcact tgggtcgaga tatcgcccaa
    14941 ctattttatt tatagaatta aacatttttc taatacttgg attttcagaa gtagatacaa
    15001 ccttatctgt attagatttt atagatgaat tattgcttaa tatattctca atatagaaat
    15061 tttttaaatc taaaaattct attgaataat taaaattatc atattctcca ttgtcatgta
    15121 tttttataat cttcttttct tcccagaatt tccaagcaga taacacatca gacaatggta
    15181 tatttaggtt tttggctatt gaattattgt cgaacttagg attagaagta atatcacatg
    15241 cttgtctata tcctaaaaga taaactttta cgtataaccc gtccgccatt ggcataaata
    15301 tatctataaa gatattggga atagtgattt cacctaaatc tatttcattg ctttctttaa
    15361 aaaacatttt atcacctcag attaaattat atcatttttt atattatatt tgtctgtaaa
    15421 caaataaaag gtcacaagat tagcatgtaa atattttcta aaagtattca ttttgaaaag
    15481 gaggagatat tttggttata aaattcaacc aaaaagtaag aagaacatta tcagtagcga
    15541 ttctatttat ggtattaata ataggttgct ttatagaaaa ggatgacagt ttagaaacat
    15601 acaaagaaaa aactaattca aaaataaatc agtataatat ggatgttata tttgatgatg
    15661 agactaagag gttaatgtgc aatcagaatg ttgactacat aaataataca aaatcaaaca
    15721 tagataaaat atatttccat atatatccga atgctttttc caagaaagat tttgctcctt
    15781 ttgaaaaaag tgaaatgaca agggcttatc ctaatggatt taacgaagga tatatagata
    15841 taaagaatat attaaataat aatagtaaaa tggagtataa aattaaagga gacaaaaatg
    15901 acatattaga aatagaatta gggaaagagt taaaaccaaa tgaaagaatg tcattagata
    15961 taaaatataa cgtaaaaata ccaaattcaa ttggaagatt tggatatgga gaaaatacta
    16021 taaatgttac taattggttt cctatagctt gtgtaaatga tgaaagggga tggaatttaa
    16081 agagttatga aactattggc gacccatttt atagtgagac tagcgatttt catgtgaaac
    16141 tattgattcc aaataaatat aaaattgctc atacaggaaa acttattgat gataaacatg
    16201 ataataagaa aatgctatat gaaatagaag ctgaaaacgt tagagatttt gcatttatac
    16261 taagtagtaa atttgatgta aacaaagtag attcaaaggg agtagctatc aatacatata
    16321 atttaaataa aaagctttct aaaaaagcaa ctgaagtggc aaaagattca ataaatatat
    16381 ttagtgaatt atttggaaag tatccttaca aagaattttc tgttgtagca agtgactttt
    16441 ttataggggg gatggaatac cctatgcttg ttatgataga ccaaagttta tataatgaaa
    16501 aaaatgaatt tttactagaa tatgttattg cccatgagac agcccatcaa tggtggtatt
    16561 ctgctgttgg aaatgatgaa ataagtgaac cctggttaga tgaagcactt actgaatatt
    16621 ctacaatagt ttattttgaa caaaagtatg gtaaagatat ggcaaacaag cttataaaaa
    16681 ctatggagat acagacaaaa agttatttga gcgaaaatat atttaagcca gcaaatgagt
    16741 ataaaaattc tactgaatac agcttgaatg tttatacaaa aggcgcaata gcatttaatg
    16801 aaataagaaa agaagttgga gataaagtat tctttgagac tttaaatgag tactataata
    16861 agtataagca taaaaatgct aatggaagag cttttgtgga actatggaat agtaaaggtg
    16921 tagatataaa taagattata agtgaatgta agtaaatagt gaatgtaagt aaattttgtg
    16981 cagagtatta aattttattt aatactctgt ttttttggta taatataatt tataaggcag
    17041 aaaaagaggt gaaggaatgc taaagtattg ttcagtaggg agtggtagta gtggtaattg
    17101 ccactatata gggtataaaa atactaatat actagtagat gcaggattaa gtggcaagag
    17161 aataactaca ggtcttaaag acattggtgt agatgcagat aaattaaaag gaatatttat
    17221 aacacatgag catgttgacc atataaaagg agcaggtata ttatcaagaa agtttgatat
    17281 accagtattt gctaatataa aaacatggtg ttctatgaaa gacaaattag gtgacatcaa
    17341 agataagaat atgaaagtat ttgaaaatga taaaacctac tcattaggtg atatcatagt
    17401 aagacctttt tcaatagaac atgatgcgtc tgacccagtt ggatataatt tttatacaga
    17461 gagtgatgaa aaaatgtcta tagctactga tataggatgt ataactcaaa atataaaaaa
    17521 acatttatat aaatctaaat tagtagtatt ggaatcaaat tatgacccta atatgcttat
    17581 gatgggttca tatacatatg ctttaaagaa aagagtaatg tcaagtactg gtcatttatc
    17641 caatgaagat gcagctaatt tctgcgttga acttattaat gaaggtacag aatctatatt
    17701 attagcacat ttaagtaaag aaaataattt tcctgagtta gcgtatgaga cttcaaaagg
    17761 agtattagca tcaaatgata tagtagttgg acaagatgta aaactagatg tactatcacg
    17821 aaatgatgtt tctaatgttt atgagatgaa gtagaggtga ataactttgc taaagagaaa
    17881 ttttgaaaat gttgaggata ataaaatttt atactctata aagcagggta tgattttagc
    17941 gatacctgct attatgactg gttcaacggc tcttgtaatt ttgaatcttc caataaaggc
    18001 ttatcaagaa tatttatcag gcttgttcaa tggagaggtt acaaatattc ttaactttat
    18061 aaatgattct actttaggaa ttatttcatt aataatatta ttaacaataa gctatagtta
    18121 tggaaaaata tatgggagca agtatacagt acttgttcct atagttgcaa tgtgctcttt
    18181 tttagtattt tcgcatggta atgagcttaa ttcttatata gaaatattta agacaaagtg
    18241 gttatttaca tctataattg tttctatgac atcttctgta ttgtttgtaa aacttacaga
    18301 atcttttgtt acaagtgtaa aatttcatac agaaggggct gatgcagact ttaatatggt
    18361 agtttcggct atagttcctt tcataatagt agtatttttg ttttctattt ttagagtgat
    18421 aatgataagt ctaattggca gttcaaactt tcaagatata ttttataatt tattttcaaa
    18481 tgtttttaat aaaatgggaa caaaccttat gagtgctctg ttgtttattt ttttaatgca
    18541 ctttatgtgg ttttttggga ttcatggtag taatgtatta gataccgtcg caaaaaattt
    18601 atttgaaaat agtatggcaa ttaacataaa tttagttaat aataatcaat taccaacaga
    18661 aatttttact aagacctttt ttgatacaat ggttttattg ggaggatgtg gctctttact
    18721 atgtttagta attgcaattt ttcttagtga aaaacgtata aatgtaagaa agttagctaa
    18781 aattgcttca ataccagctc tttttaatat caatgagatg ctagttttcg ggataccaat
    18841 tgtttttaac tatattatgt ttgtaccttt tgtaattact cctataatct taaccatcac
    18901 aagttatgtg gctatgtcta caggtattgt accatgtaca gtttcagctg tagaatggac
    18961 ctcacctata tttttaagtg aatatatgtc tacaggttca ataagaggta gtttgttgca
    19021 agtatttaat ttatgtgttg gtgttatgat ttatattccg tttataaaaa tgtcgcaaaa
    19081 tagatataca tatatgttta aaaataatat agaaaattta acaaacttat taaaaaggta
    19141 tgaattaaca ggtgaacagc ctaatcttct aaaccataat ggtaaaatag gttcaatttc
    19201 aaaaatgttg gcatcagact tgaagtttgc tatgaaaaga ggagaaatag agctttttta
    19261 tcaaccacaa gtcaattata atggaaatgt tgtgggagca gagggattac ttagatggaa
    19321 acattcaata ggagggttca tttatccacc actagttata attttggcta aagaagaagg
    19381 gttcttagat gagcttggag agtatattat taataaagca tgtattgatt taaagaagag
    19441 tgaaaagcta ttgagaaatc ctgttaagtt ctctgtaaat atatctccag accaacttga
    19501 tgaccctaag ttaccagagc aaataaaaaa tatcattagt agtaatgata taaatcctaa
    19561 tatgttaggt atagaaataa cagagcaaat tgcattatca ggttcacaaa taattattga
    19621 acgtatcaat gcgatacata acttgggaat aaagttaatt atggatgatt ttgggatggg
    19681 gcatagctct cttatatatc ttcaaaataa taactttgat atagttaaat tggatggttc
    19741 attagttaaa gagatactta caaataaaag aagtagtgaa attataatga ctattgtaaa
    19801 tctttcaaaa aatttagatt ttgatatagt tgttgagtat gttgaaaata tagaccaaag
    19861 ggataagtta tatggtttag gatgtgaaat atatcaagga tattattata gcaaagcaat
    19921 accttttgaa gagtttgtag aatatgcaaa tcaagaaaga gagatataat tttataagag
    19981 cttgtaaatt ctagtaatgc atagatttgt aataaaatta agtttaggag aaatatatga
    20041 atataagtat agtagtagta ggtaaaatta aagaaaaata tttaaaatta ggtattaatg
    20101 agtttaagaa aaggctttcg aagtattgta agttagaaat tatagagttg gatgatgaaa
    20161 aagctccaga gaatttgagt gttaaggaaa tggaaattat aaaagacaaa gaaggaaaga
    20221 aaattttagg gaagataaag cataatagtt atgttatagc cttggctatt gatgggaagg
    20281 gtttatcctc tgaagaactt gctaagacta tgagtgattt agcagttaga ggtaatagta
    20341 gtctctgttt tataatagga ggttctctag ggctttctga tgaggtttta ggaagggctg
    20401 attataggtt atctttttct aggatgactt ttcctcatca gatgatgaga ttgatattgt
    20461 tagaacagat ttatagggct tataggataa ataatggaga accgtatcat aaataaatga
    20521 tacggttttt gttagtttgc aacttaataa tattaaaata acaaaaaagt attgaaagta
    20581 tattgcattt aaggtagaat taaataaaat attataacat tataatgttt atatagttgt
    20641 taataaaaat ttggaggaaa atatatgata aaaataatgt tggcctgttc agcaggaatg
    20701 tctacaagtt tattagttac taaaatggaa agtgcagcta aagaaaatgg tatagagtcg
    20761 caaatttggg caataccaga atcaactata caaaatgaaa tagaaaaatg tgatgtatta
    20821 ttactaggac cacaagttag atatgtatta cctaaggctc aagaaattgc taagccatat
    20881 aatataccag tagaagttat aaatatgatg cactatggaa cagttaatgg agaagctgtt
    20941 ttaaatagag cgatagaact taacaacact aagtaatata aattataatg tatttttaat
    21001 atataaattg ggagaatcaa aggatggatg aaaagattat agaaatatct tttaatataa
    21061 taggttatgc aggtgaaggt aaagggttag cttttgaagc aataaaagag gctaagaatg
    21121 gaaatataga aaaagcaaaa gaattattaa aagagtctag ggaagttata aataaagcac
    21181 atagatatca aacagaatta atacaaaacg aagctagtgg aaacaggact gaaataagtg
    21241 tcatacttat acatgcacag gaccacctaa tgaatggtat gaattttcaa cagttagctg
    21301 aagaaatcgt tgatttacat ttgaaattac aagataaata aaaattaaag agaataaatt
    21361 aagagagttc ttaaaagata cattatgtgt agcacactaa ttgttagatt attgattcaa
    21421 tatttagtga gtaatacatt aggattattt tgaggactct ttttatattt agtttaaagt
    21481 taataaatat ttattattaa taatataaga ttatatgtta taatattata acatataatt
    21541 taagaggtga atcatgttaa gaaatcaacc attatatata caagttgtag atatattaaa
    21601 gcaaaaaata ttaaataacg aatataaaat tgatgaaaat attccaacag aaagggagtt
    21661 agagcaagag tttggagtca gtaaaattac tattagaaaa gctattgaga tattagaatc
    21721 agaagggtat gtagagaaaa aaagtggaaa agggacaaaa gtaataagta atagcctttt
    21781 taataagcta tcaaaagcag agtctttttc aagtatactt ttatctaaag gtcataagtt
    21841 atctaaagag actatttcaa ttgaaaaaat aataaatgat acaaattcac aagtatttag
    21901 tatatttgga gaaaaatgta gcaagattac tagaatgtat tacttagata gtaagccata
    21961 tatatttttt actcattata ttccttttga tgaaaatcta actgttgaaa gttttaataa
    22021 tgaaagttct ttatatatgt acttatatag gagaaatata aatataacta gatttgaaga
    22081 tgaatttttt atagaagaat cagaactaga ggtatcaaag gctctaaatc tagctaaaga
    22141 catattatta ggaagaaaaa gaaagaccta tgatgagaat gataatatag ttgagctatc
    22201 atttgcaaga tataatacag aagtacaaaa ctatgtaatt aaatatgaaa tataataaga
    22261 atcctgagtt tattgaaatt ataatatgct caggattttt gtttagttgt aaaaacaaaa
    22321 aaatattata acattataat gtttacaagc aaacattata atgttataat atttttaata
    22381 aacaagtgat attatcaatt acataaaaat tagtttatgt tatgaaatcg ttattgcatt
    22441 ataaataaag gataatatca ataatacagg gggataacta tggaaaaagt cgcaagtttt
    22501 ttagagcgca agattatgcc tgtagcagcg aagataagta atcagagaca tatgagagct
    22561 gtaagagcag gtataatagc aacattacca ctaacaatag tggggtcatt tttcacaata
    22621 ttattaaaca tacctattga tgcttatatg gattttatag ctccatttaa agcaatatta
    22681 gatataccat ttagatatac tgtaggattt ttatctctat atgctacttt tgggatagca
    22741 tcatcacttg ctaaaagcta tgatttagat acaacaggtg ttggtatgct tgcagttatg
    22801 gcatttttag tttctacaat agttccaact caagtagttg aaccagtagg tggagttata
    22861 gaagctggta gatggatgga tatggcaaaa cttaattcgc catcattatt tggagctatc
    22921 gtaacatcat taattacagt tgaaatatat cgttttatga aggaaaagaa tattacaatt
    22981 aagatgccag caggagttcc agtagaggtt tcaaattcat ttactgcatt attaccgaca
    23041 gcagtaattt taatgttatt ctgggtaata agacatattt taggatttga tataagtgtt
    23101 gcactaagcg ctatgttatc acctttaaaa ggattcttat caggaaatag tttaggtggt
    23161 gggttactga cagtattcct aataacttta ttctgggtac ttggaataca tggtccagct
    23221 ataatgggac cagtaataag acctatatgg gacatgtcta tagcagaaaa tataactgca
    23281 ttcacagaag gagtatcagc tcatgcaatg ccaaacatat ttacagaaca atttttacag
    23341 tggtatgtat ggataggggg agcaggggct actcttgcat tagttgtaat gtttatgttt
    23401 tctaagtcac aatatctaaa aagtttaggt agattaggat ttttaccagg gttattcaac
    23461 ataaatgaac ctataatatt tggagcacca atagttatga atccaatact agggatacca
    23521 tttataattg gacctataat aatgacaatt ctatcatatg ttcttactat tacaggggtt
    23581 atacctatga tggtagcaag attaccattt gcaatgccag caccaatagc agcagtaatg
    23641 agtactaact ggagtatact agcgggtatc ttagtattaa tcaactttgt gatatcattt
    23701 gctgtttatt atccattctt caaagttttt gaaaaacaac aattacaaag agaacaagaa
    23761 gaaatgagtg aaaatttata aaaatacata gatatagagg ttttgtacta tataaaatat
    23821 agaaaaaact tataagttat taagggtgaa ggtaggagga atgacctttg cctttaattt
    23881 tatatatttt atataatgta atttgaggag gagaaagatg gaaagaagat taggaatatc
    23941 agtatatcca gagcatagta acatggaaga tgataaggca tatataaaaa tggcttcaaa
    24001 atacggattt aaaagaatat ttatgtgcat gctttcaatt acaagaccta aggaagaagt
    24061 taaaaaacat tttaaagaga ttataaccta tgcaagagaa ttaggatttg aagtaatcct
    24121 tgatatagct ccaaacatat ttgatatatt acaaatatct tatgatgatt taacattttt
    24181 tagtgagtta aatgctagtg gaataaggct tgactatgga tatgatggaa gtaaagaagc
    24241 tatgatttct ttcaatccat acgatttaaa agtagagcta aatatgagta atgatgtagc
    24301 atacttggat aatataatta cttacaagcc aaatgtaaat aatatatatg gttgtcacaa
    24361 tttttatcct caaaggggta caggcttacc atatgatttt tttgtaagat gctcaaatag
    24421 atttaaaaag tatggaataa gaactgctgc ttttgttaat tcaaaatacg caacaatagg
    24481 gccttggaat ataaatgatg ggctgtgtac gcttgaaatg catagaaact tagatattgc
    24541 tacagcaact aagcacttat tcgcaacagg attgattgac gatgtaattg tagggaatgc
    24601 atatgcaagt gaagatgaat taaaaagaat gtctgaagta aataaatatc aagttgtatt
    24661 taatgtagta actgaagaaa aaacttctga agtagaaaag acaataatgg caaatgagca
    24721 acactttaga agaggggata taaccaacca agtagcaagg tctacagaag taagaaaaaa
    24781 atatgctaaa gaaaatttcc ctaagcatga taatacgacc atctttaaaa gaggagatgt
    24841 agtgattggt aatgatgact ttggaaagta taaaggggaa ttacagttga tacttcaaga
    24901 ttgtgcagat gaaagaaaaa ataaggtagg tagaataaag gaagacgaaa taatgctttt
    24961 agactttatt gaaccttgga gtaaatttaa acttatttta gagggagagt aaagtgaaga
    25021 tagacatatt aataaaatgt ggaaaaacag tagataaaag tataattgat atagctatat
    25081 tagacgataa aataatagaa gtaaaaaatc agatagatga ttctaagtat caggcaaaac
    25141 aaattataaa cttagatgga gagaaattta ttagtgcggg ttggatagat atacatacac
    25201 attgctacga aagactagac ctttataagg attatccaga tgaggttgga ataaaaagtg
    25261 gagttactac tgtagtagat gcaggaacaa caggagcttt agatataggt aaattttatg
    25321 aagatactaa aagatataag acaaatgtat atgctttaat aaatattgct aagcagggaa
    25381 taacatctca agatgaactt tcaaatatga tgaatattga tgaatatgaa cttagaagag
    25441 cagttaaaaa atacaaagat tttgttgttg gaatcaaagc tagaatgagt aaaagtgttg
    25501 taatttcaaa tgatgttgaa cctttaaaag tagctaaaag aataaaaaat gaacttaatc
    25561 tacctatgat ggttcacttt gggagttctc ctccaacgat agaggatata tttgactata
    25621 tggaaaaagg agatatatta acccatatat ataatggaaa gccaaatgga atacttagag
    25681 gcaatgaggt taaaaaggaa atcatagaag ctagagaaag aggtataatt cttgatgtag
    25741 gtcatggaac cgaaagtttt agtatggata ttgcaatgaa atctaaggat gcaggaattt
    25801 ttccagacac tataagtaca gatatatata ttaaaaatag aataaatggt ccagtgtata
    25861 atctatctac aacaatggaa aagtttatat atatgggcta ttcacttgaa gatataatag
    25921 ataaagttac taaaaatgca gcagatgcaa taagtttaaa aaataaaggt ttaataaaag
    25981 aaggatatga tgctgattta actatatttg atgtggttaa tgaagaaaaa gaacttcaag
    26041 attctttgaa taagtcagta atcacaagta cgtctataaa gccaagagca gtagtagtca
    26101 atggagaata tttaaatata ggtaagtcta tagaagagaa tgagtagaag ggagaaacta
    26161 ttgtatgagt agtatatttg aaaaatactc tgtaagacgt gttataaatg cttcaggcaa
    26221 aatgactata ctaggagtat caacagtaga taaggaagtt attgaagcaa taggtgaagg
    26281 gtgtaaaaat ttctttataa tagaagaact tataaataaa acaggtgaat atatagcaaa
    26341 tttattaggg tgtgaaagtg cactaatagt ttctagtgca tctgctggga ttgcacagag
    26401 tgtgggagca atgatagcaa aagatgatat gagtctagtt tacaatgtaa ataatcctaa
    26461 gaaacaagtt aaaagggaaa taataattcc aaagggacat aatgttgatt atggagttcc
    26521 agtagaagta atgataagac ttggaggagg agaagttgtt gaggccggtt atgctaatat
    26581 gtgtaacaag gaacatataa taagagagat aaatgaaaat acagcagcaa ttatgtatat
    26641 taaatctcat cattgtgttc aaaagagtat gctaagtgta ttagatgcag tacaagttgc
    26701 taaagaatat aatttacctt tgatagtaga tgcagcagca gaagaagatt taaatgtata
    26761 ttatgaattg ggagcagacc tagtgatata cagtggtgct aaggctatag agggtcctag
    26821 ctcagggtta gtaataggta agaaaaaata catagataat gtaaagcttc aatcaaaggg
    26881 tataggaaga gctatgaaaa taggaaaaga aaatattgta ggtctaacat gtgcaattga
    26941 gagatatata agtaatgata aagtaacttt aaaagaaatg gaggacaagc taaatccatt
    27001 tatagacaag ataaatacta taaagggagt gagtgctagt ataactaggg attcagcagg
    27061 tagagaaata cttcgtggag aaattgactt taatgaagat ataataaata agtcaacaca
    27121 acaaattata aatgaactta gaagtggaga gatagctata tacactagag actataaggc
    27181 taatgaaggc aagattgaga ttgatataag aagtgtaact ggaagtgatt tagatactat
    27241 ttataatagg ataaaaaata tagtagaaag ttagagtgta gagtaagtga cattataatg
    27301 atttaataaa ttaaatttta ccaatggagg ggataaatat gaattgtata ccacaatatt
    27361 ataaaaatag agtttgttta aatgttcttg caggaagcat aaaaaatgct aaagaagtat
    27421 atgatgcttg tgacgggcat gtattgatag gagtactatc aaagaattac agcactgtag
    27481 aagcagctat tgaagatatg aaaaaatact caaaggaaat agataatgca atatctgtgg
    27541 ggcttggagc tggagaccca aatcagtcaa atatggtatc tcagatttct aaagaacttc
    27601 agccacaaca tataaatcaa gtgtttacag gtgtggctac gtctagagca ttacttggtc
    27661 aaaatgatag tataataaat ggattagttt ctccaactgg taaaccagga tttgtaaaaa
    27721 tatccacagg tccattaagt tcaaaagaaa aagatggtat agttcctata gaaacagcaa
    27781 tagctatgat gaaagatatg ggaggaagct caattaagtt ttttccaatg ggaggactta
    27841 aaacaaagga tgaatatata gaagttgcaa aagcttgtgc aaagcataat ttttatttag
    27901 aaccaacagg aggtatagac ttagataact tcaaagagat agttcaaatt gcacttgatg
    27961 ctggtgtaga aaaagttata cctcatgttt atacatcaat aattgataaa gaaacaggtg
    28021 aaacgaaagt ggaagatata ggagtacttt ataaaataat gaaagaatta ttgggataga
    28081 tttatgaata tattagcttt tggtgaaata atgatgagac tttcagtacc tgactacaag
    28141 ttcttaacac aaaccaatga actaaattat ataattacag gcacgggatt aaatatatta
    28201 tctggactta aaaactttgg atacaatacc tatatgttga caaagttgcc taataataat
    28261 gtaggtaaag catcaagtgc aaatataaga aagcttggag taaaagatga ctttatcaca
    28321 tatggaggga atcatatagg agtgtatttt cttgaaaatg ggtatggaga aagaccttct
    28381 gaagtgactt atttgaatag gttaaatagt tcattttgtg agagtaaatt acaagattat
    28441 gatattgata aatgtttaga agaaatggat atagtccata tttgtggtat tgctatgcaa
    28501 cttacagatt caattaaaga gattgcttta gaactagcta gaaaatctta tgaaaagggg
    28561 ataaaagtat tctttgattt taattttaga caatcattaa atccagaaag gacttatgat
    28621 gatttagtaa atgattataa gaaaatacta ccatattgtc atgggctgtt tgctagttat
    28681 agagatataa ataagatact taatatagat agtctaaata taaaatctag ttcagaattg
    28741 attaaaaaag agtatgatat aggtattgta gctggaacta tgagaagatt tgatgatatg
    28801 aataatagat ttataaaggg atatatatca aatgatgatg gttattttga aagtagggag
    28861 tataagttag aaatatatga cagggttggt gccggagatg gatatgtagc aggaattata
    28921 tattcatatt tggaaaatat gaagagtgaa gatatagttg agtttggggt tgctagttcg
    28981 gtgttagctc atacaactta tggggataat tctttagtaa gcacaggtca tgtacttaga
    29041 ctgatgaaca atgaaaaaat agatatattg agataataca tttgtatata ataaaatttt
    29101 tatctttttt agacacattt ttgacttgct tatatttgtt aaacagagtt atttttaacg
    29161 attataaata ttaaattaaa agttaaataa taaaatagta gaaaatagca ttaactatag
    29221 aaaaaatggt taatgctatt tttatcgtta atattgatta attttatatt atagaaatga
    29281 acttttctaa tgctggattt gtattttttt tattccatac aactacaact tcaattctat
    29341 tttctacttc ttttataggt actattgcaa tatcacgttc tatcattgta tctgtaacac
    29401 aaaatgaagg taaaatggat attcccatgt tagaagatac caatgattca atagtattaa
    29461 gattatttga acgttgtacc acattaggta caaaccctgc ttcacgatat ctattttgta
    29521 tcattgactt ttgtccatac tcatctcctg tttcctttac aaaaataaat ttatcatgtt
    29581 gtaattcaat taaatcgata gaatcctcat tgtaaagtgg atgaccagga ggtaaaagag
    29641 ctaccaaaga atatctttct acagttttat attcaaaatc ttttaatggt tgattttctg
    29701 gattaataac aaaagcgagg tccatagaat tattttttac taagtttaaa agttcggtta
    29761 tttcattagt acaaatatcc atagatatat tagggtatgt attagaaaat tcttttaagt
    29821 attgttgaag tttgtttttt tcatatccta ttaaggttcc aatgctcaaa gttccaaaaa
    29881 aaccatgtgc aaattgttgt gtttttgcaa tagcatcatt gatatttttt attatagtac
    29941 gtgcctcaga taaaaaaact tttccagctg gtgttagttc aacatgacgt ttacttcttg
    30001 taaatagagt tacatttatt tgttcttcta aagcttttat ttgctgtgta acagcagttt
    30061 gagaaatata aaatttttct gctgttttgg taaaacttag attttctgct acagatataa
    30121 aatattctaa ttgtttagta ttcaaataat cacccctatt taaacttgat tgtttgtaaa
    30181 ccttatttat ctataattaa agcttattat agtataagaa aatttacttt taaagaatat
    30241 tagaaaatat ttgtataaaa atatatgttt taagtatatc tttacattag attttataaa
    30301 tttattaata agaataactt atatattcca atgaaaacga tattggattt aagtaagttt
    30361 gtctcataaa atgaaagtat atcaattgat aatactttaa ataagaaggg agaaaaaatg
    30421 gaagataaat tttatgcaaa aggcaacgga aataacggat atattaaaaa tcttgaagtt
    30481 tgttccttta ataacttaga tggaacttgt ggaatgtttc aaatggctct gtacaaaaga
    30541 gatgaaaaat actatttata tggatgctgt tttggaggaa ataaaaaaaa tggagtaatg
    30601 attagcgata ttacagaccc ttataatcca caatttataa aacattttca aatgttagac
    30661 cctaaagagt atcctacaac aacaactccc aaaattcaaa tagcagatga tttaatgata
    30721 gtagcaatga gttgtggaag tggaccagga gcacttgttg accaagctaa attagcaaat
    30781 attaagtgtg aagcaggaat tagaatatac agtttaaaag aaaacccttt aaatcctaag
    30841 tttttaggat attgggattg tggcttaaag catgtaatgg gtgttcatag attcatgtac
    30901 aatggtggaa gatatgtaca tttatcaagt gattgtgttg gctttgaagg tctgatttat
    30961 agggttatag atataataaa tcctactaat ccagtggaaa taggtaaatg gtggagacca
    31021 gaccaatatg cagatggata tccaaataga acttttgatg caggagcacc tcattgccca
    31081 gaatttatgg ataaaggatg gcttcatgga cctccatttg taagagatgg aaaagcatat
    31141 tgtggttatg gaggagctgg tttagttgta ttagatgttg aagatttaac aagaccaaga
    31201 tgcttaggtg aattgccatt tacgcctgca ttttctagta gacttgcagg tgcaagaact
    31261 catacagcat taccattgcc aggaagagat ttagtcgttg ttcaaaatga gggagaaaga
    31321 ttccagttct ttaaaccaga taacattaca gatgttcaag ctatgaataa tatacatatg
    31381 gttgatgtta gtgacccaac aaaaccaaca ttaattgctc aatttccata tcctgaagtt
    31441 ccaaaagatt tcccttatcc taactttaat gttgcaggat taggaaaacc agggccattt
    31501 ggtccacata atcttcatga accaatggat aataagccat ggttagagca aagaggagat
    31561 agagtatatt gctgttattt ccatgcaggg ctaagggttt atgatgtatc agacccatat
    31621 tatatcaaag agctagcata ttttatacca ccaaatccaa ataaaacacc agaagaatct
    31681 tatttcccag gattcccagg accacgcttg gcagtaacag aagatcttat cgttgatgat
    31741 agaggctaca tcatcataga tgctttagat gatggattct atatattaaa aatgaaagaa
    31801 gattaaataa atactaaaga gggagagttg aaaatgaaaa tactaggtat ttcttttgga
    31861 acaaaaaatg gaaataatga cacaatgtgt aaagttgcac ttaaaggtgc caaagaagca
    31921 ggggctgaag tagaatttat tcaaatgtca tctattaaca ttaaacattg tactggatgt
    31981 tgtgcgtgtg ttaaaacttt attatctggg aaaggtagta tgtgtgtatt aaaagatgat
    32041 tttgaatggt tgcttgacaa aatgaaagat gcagatggta tagtagtttc agaccctatt
    32101 tttgaagaag gggcatcagg attattccat actataatgg accgttttgg acccagagca
    32161 gatagaggaa ataacattat aggaacaaaa gttgcagaag ctataggagg aaaaattcca
    32221 gattcaagaa tgcttaatga taaagtaata tcatttatgg gaataggagg ttctgactgg
    32281 ggtacaagag ttcaatgtga acatgctatg ctagctctca taccaatgtg gaaagttata
    32341 gataatgctt ggttcccatg ggcaaaggaa ttagtaatgg atgatacaag gcttgttcaa
    32401 gttcatcaaa ttggtttaaa tatagttgaa gcagcaaaag attttgaaaa agcttcatat
    32461 caaggagaag aaggtgtatg tccacattgc cacaatagat tattctacct tgagccaggt
    32521 actaaaaagg caatttgtgc tctatgtggt atagttggag agttagatac tataagtgga
    32581 aaaactatat ttagttttcc agaagagcaa ttaggtcatg ctcatgatac tttaccaggt
    32641 aagtttatac atggtgatga tattaagaaa atggaaggtc attatgcaga agttcgtaaa
    32701 acacaagaat ttaaagaaag aaaaacatct tataatttta tagagacatt aactaaagaa
    32761 aagcaagctg gagtttcatt aggataataa ttaaaatact aagactcttt tttaagggat
    32821 gttagatgtt ataggaggct tattgtatga atgaaaagcc aagttttaaa caaattgaag
    32881 ttattttagc agttattttt ttggtactta catattacaa ggttagatta actccagcat
    32941 ttattgttat atttatacca ctatatttaa gttattttgc agtatctatg attgtaaatc
    33001 aaatagcaat taagctatta gagatagggc aagctaggga agctactaat tacaatatag
    33061 gaaatggata gaaagaaaaa agaaatatta acactgtcta tggggattgc tttactacct
    33121 cctttatggg cagtattagc accttatata ggaataaaaa ctggagctgt agcactaatc
    33181 tgtgcaggat tatatgtaac taatggtaat aaacaaaaag atggattaaa aataatgttt
    33241 ggtttttggt gtggagattt atgggcagtg ttagccattt taattatggg atatatgaat
    33301 ttcaatcaaa acctagaatt atttttaaca cttagtatat taggtttttt tgcagttgta
    33361 attgcatcct tatttgaaaa aataattttc ctaccatcat ggctttgtgg ttgggctata
    33421 ggtctaactg ttatgacagg tgaaaatatt attaatttac aagatatttg tattcaaatt
    33481 gctgtagcta tggcagtagg agtatggtac gtgggtgcag gtgtagattt gtttacaata
    33541 tatattttga gtaaagatag gaaaaaggga aattgaaagc atgtgaatat actttgatta
    33601 taggagaatg gctatgggtt ttgaatttaa aataatgaga agtttaatat atgtaggact
    33661 tgccaaggaa gaatatagac ctaagctaat ggactggtta tatcgtcacc atattccaga
    33721 tagtattagc acttttggac catattgtac taaatatgcc ttttatcaag catatcctac
    33781 accaaatgaa ggtgagcgtt ttggtgcacg taagatgcaa ctaacagaac attattggct
    33841 tgtagatgaa catatgcctg agatggcaaa tagaattatg acagaatata tgcctatgga
    33901 tgttctacgt tggcaagggt gtataccaga tgtagaaaat aaaagggttc atgaaaatgc
    33961 agaaagtgga gatgcaggac gtgcagtagg tggagataat ggatgtccac catttatatt
    34021 tgcctttgtt ccaataaact gggaagaaga ctttagagga aaaggacgta ctgtacaaga
    34081 tggaccaaac tatcgttggc aatttatgat taagtatcca gatggtatct ctaaagaaga
    34141 aggagaaaaa tggttctatg atgaggtagt gccatacttt acaaactgtt gctatgttaa
    34201 tcgttttgtc agtagtaaaa taatgattaa ttatggagca actgcttttg accgtgtgtc
    34261 agaactatgg tttgaagggg aagaagaatg gtataaagct gtggttgaag agacaaagtc
    34321 gtttattaaa aaaccagaat gggcacaaga agaggagttt ccatatttaa aaccacaatt
    34381 caatatcgca tcagtattct taggtgatat agcaactatg gatgcatact cacagtatcg
    34441 tggatatata ccaatgagat aaaaattata gtttgtgata agtagaggag gcatttactc
    34501 ttactggtag aagaagactt attgtatcct taaaagtagg tgaatatata gttttatatt
    34561 taaatttata taaaactaag actaataaat atgaaacaat agaattagaa taatttgata
    34621 atatttgtta aaagagcatt tagaatgtgt ttaaagatta agatattcct tttatggtag
    34681 cagatagttg aaataataaa actgttatca tgaaaggaat atttttatgt ataggaaatt
    34741 tttaaaaata aaagttaaat atgtttaaga acatcttaaa gtgaaagaat cattgttaga
    34801 aatatgtaga caatatagtc tttataacgt atcatattaa caagtttaca cttggattat
    34861 ggaataaaaa gagtaattcc aaaggttgta aatactatgc tgactttgat acttcaaagt
    34921 ttaggtgatt ttgtaattgt taatagaaat acgtttaatg agcataaaga atttatctaa
    34981 taataggaga atataatgca tacaattgga caagtatcta aatttttagg aatatctaga
    35041 gatacactaa aattttatga gggcaaagga ttagtaaatc ctaagaaaaa tgatgaaaat
    35101 ggatatagaa tatataatca ggttgatatc tatgatattg caacaataaa cttttataga
    35161 gaaattgata tagaaattaa aaagattcaa gaaataagaa aaagtaagag tataaacaat
    35221 ttagaactct tactagaaga gaaggaacaa atcatactaa aagagataga atataaaaag
    35281 cttctcttaa aaaaggttca gttagtcaaa gaagattgtg acaaaatcaa aacatatttt
    35341 ggaaaataca caataaaaga aatgaagcct ttggaagtaa aaggtaaaat aaaacatgtt
    35401 actaactatg aggaatatga agccttaaaa gagaatacaa acaacttaaa aaaagcagtt
    35461 acattaactg atttaagaag agtaataaat tttaatgaag aaggtataat agaagaaaaa
    35521 tttataattg taagggaagt agatgactac aacaaaatca tagaaggtga agttctatct
    35581 catcccaatt gtatttatac tattattgaa gatggcagat ggtcaactgg aggagaaaat
    35641 attgataata aagtggaaga cagattaaga gaaatagcaa tagaaaatga atgtgaatta
    35701 cttggaatag tctatgttaa tatattgctt actacttatg aagaagggat tgagcgtata
    35761 tttttagaga tttatgcacc tataaaataa tataaaagca ttgacctagg acttactcat
    35821 aggtttaaac tcatgatata agcagttata acttatatag caattaaata ttaatttgaa
    35881 atgaggatta cttatgagaa cagagaaatc attaaaaagg cctatagcca gttttattat
    35941 tctaacaaat ataatttttt taccactttt tttattggta gtagtgacta taatgttaaa
    36001 acttccaaca gtggtatctg acattgcgtt gtgtatatca gcgtggtcat ccacttttgc
    36061 atttatgata ttatttaaaa aaatttatcc aggtaagaaa tttttagtac acgtaaaaga
    36121 tagatttaga aataaactta aattttcaac agtatttata ataattagta tacaagtcct
    36181 aatagctgta gtagttatat ttatattagc atctaaaaat catggaataa tgcctaactt
    36241 tacagtttca tcatggggag catttatata tttattttta aaaaatttat ttgcaggatc
    36301 attaggtgag gaattaggtt ggagaggatt tgttcaaaat catcttcaga aaagatattc
    36361 tcctttaaaa gcatccataa tagtaggttt ttggtggggg atatggcatt taccaatatg
    36421 gtttacaaca ggattcaaag ggcttgattt aataaaatat attatatttt ttatgattgc
    36481 aattatatca atcagtatta taatgacaac tttttataat ctaaataaaa ctttgataat
    36541 acctattgta atacatcaac tatttaattt tttaataggg ataattaatg gagatttaat
    36601 agagcttata aattactatg caatgttata tttaatattt gcaattgcat taatagttat
    36661 aaatccagga aatgtattat ataaaaatat tagagagtaa tatatatctt tatggtaaga
    36721 tataaagaaa ttattagaag aacatccaag gaggaataaa catggtttta ctgattgttg
    36781 atgcacaaaa gttaataaca aacgagaggt tatataaatt taatgaattt gtagtaaatg
    36841 tagaaaattt aattgataca gctagaaaaa ataatataga agtaatatat gtacgtcatg
    36901 atgatggagt tgaaaatgaa ttgactaaag gtaaaaatgg ctttgaaata tacgaaaaat
    36961 ttaaaccatg taatggagaa aaaatatttg ataaaaaagt aaatagtgct tttaaagaaa
    37021 ctggtctatt agaataccta acaaataatg gtgaaaaaga tattattatt gctggtctcc
    37081 aaacagatta ttgtattgat gccactataa aatgtggatt tgaacatgga tttaatataa
    37141 tagttccagc ttattcaaat acaactgttg acaatttttt tatgtcagca gaacaatcat
    37201 ataaatacta caatgaattt atatggaatg aaagatatgc gaagtgtatt tcattagagg
    37261 aaacacttag aaaaatgaaa taaaaataga gtatgaattg tatatgtaca taagttgatt
    37321 tcataaaaaa ggatgttaaa ttccaagtta aaaattagat taaatatatt atattataga
    37381 taaggcacat caattttatt ttaaattaat gtgcttttta attgtagtaa tttatttata
    37441 atcaatatga tttttattat ttaaatcaat ttttcttgag tttctttgtc taaagaaact
    37501 ttttttattt ataaaatcta cagttatatt atagtatcta ttttagagaa gtttaatttt
    37561 tgtatcaatc taaaacaaaa gttatactta aatgaaattg aaaacgataa aattctcaaa
    37621 taaaataagt attgtggagg tgatgaaaga atccaatgat aatatgaaga ctaatcttaa
    37681 taagaaagta ggggttaatg gtagtggaca aagaaattgt atcaattctc cagattttat
    37741 taaatagcac attgattaca actaatggat tacaagagga gtttaattca tcaaagagac
    37801 aaattactta tagaataaat aaaatcaatg acatgcttaa agtaaaaaat gtaccactta
    37861 tatgtttaag agctgataaa gatattatcg ttagaaaaga aacaaaaaaa gcaattaaag
    37921 aaattttaga aaaaaactat tctaagaata cttattattt tagtaagaca gaaagacttt
    37981 tatatatgta tttaatgctt tttattaaca tggaggatat ttcaattaat cattttataa
    38041 attctataaa agtaagtagg tctactgtaa atcttgattt caaggatttg ataccaaagc
    38101 ttgaaaagaa gaacattaaa gttaagaaca atcgtataaa tggatattac atagttggca
    38161 atgagatgga aattaggaga gtgcttatta aaaatattat agaaactttg tctaatgaga
    38221 aaagttcaag ggtatttgat atttttatta aagaatataa tttagatagt tttgaagagg
    38281 ctaaaaaaat tatattaaag ctaataaaaa aatatgaaat tacttttgtt gaagatagat
    38341 taatagaatt tatatatata tttatctttc taaaagcaag gatgtattca actagaatag
    38401 ttactcagga aaatgtagat attcctaata tttcagttat gagttcaatg aaggagtata
    38461 aatttgctaa ggaattatta gaagtatatg ggagtgaaga taagataaag cctcacaata
    38521 taatgtatat tagtgcttgg attttaggta tatcagtagg aaattatgaa gaagatacag
    38581 aagataagga tgtaatttca aaaattgtaa ataaaatgat gacaaagttt ggatatttaa
    38641 gcggagttta ctatataagc caagaaaaaa tatttaaaca attatattct cattttagac
    38701 ctgcctacta tagactttta tttaaattac ctatttataa tccgatttgt gaaaaagtaa
    38761 aagaagaata taaattagtt tatagaattg taagtaatgc aatgaaagag ttttgcggat
    38821 tatttggaga agaaattcag gaagaagagt tagcatatct tacaatgcac tttgcaacac
    38881 ttttttcaaa taaaaaagaa tttgatggtt ttagaaaaag aatagcatta atagtttgtt
    38941 caaatggcgt tggtagttca gctattttac atgcagaact tacaaactta tttcctgact
    39001 tacactttat accaatgtcg gaatcagttg gattagaaaa tataagtgaa cctgtagata
    39061 ttgtatttac tactaattat aacgcagagg gtttaaaaac agatgtacca gtaattaaag
    39121 taagccctgt tatgaccaca aaagaaaaat ataagattac acgtgaagtg tatattcaat
    39181 taggtgatat ttttttaaga caacctaaaa tagatgaggt tatggatata ataaaaaaat
    39241 atgcaaatgt aacctctgaa agtattcttt caagtgagct attagcttat tttacacaga
    39301 tagagaattt tacatctaag gaaggagagg ggcctatgct aagtgagatt actaatgaaa
    39361 cactagtaaa aataaaggta aaagctaaga attgggagga agctattaga cagtcagctt
    39421 ctgtattagt tgaaaataac aaagtaactg aagggtatgt agatgctatg attaactcag
    39481 caaaagcatc tggaccatac atagtaatta caaaacatgt tgcacttcca catgctagac
    39541 cagaagctgg gtcaaaagag attgcaatag gcattgctac tttagatagt cctattgagt
    39601 ttgggaataa cgaaaacgac cctgtaaaat atgtattttg tctaagtgca atagacaata
    39661 atagccattt acgtgctatg tctgagctag ttgaactatt ggaagaagat gagtttttta
    39721 aggttttaga tagtgctaaa gaagctaaag aaattattga ttttataaaa acacacgaat
    39781 tataaaggag gatttaatta tgtataaaat tttaatcgca tgtagagcag gagttggctc
    39841 aagtttaatg ttaaaaataa agactcaaca aattattaag gaaaataact ttccaattga
    39901 agtagaacat ggttcattgg attcactaaa tggattcaat ggagatgctg tagaaacatt
    39961 aattgatgtt gctgaagaat taaaaaataa aaatttgaag tttgatataa ttgggattca
    40021 caatattgtt gaccgtaatg aaataaagac agaactagaa aaattccttg cttcaaaaga
    40081 gggataaatt taagatgata aggaggaata tttatggagt ttattattag tttgctgagt
    40141 acacctgctg ttttgcttgg cgtagttgca tgtataggat tgattcttca aaagaaatca
    40201 gctgttgaag tatttacagg ctcttcaaaa acacttatcg gttttttgat atttggtatt
    40261 ggtgcctctg ctatgacagg agcacttcaa aatttcaata aactgttcca acatggcttt
    40321 ggaattactg gtgttatagc ttcaccagaa gcagctacag cacttgcaca aggtacatat
    40381 ggttttgcag tttcatgtac attaattctt ggattcatat taaatttagt atttgcacgt
    40441 attacaaagt ttaagaatat tttctttaca acaggtcata gtttgttctt ttcttgtgta
    40501 ttagttttaa ttatgaaagc acatggattt gataatacaa tcactatttt aataggtgga
    40561 actatcttag ggtttatgtc tgctgcacta cctcaattct gtcaaccatt tatgcgtgaa
    40621 ttaacaggtg gcgacgaaca agctattggt cactttaaca tgattggtta tggtttatca
    40681 ggttatattg ggcgtttatt ttcaaaacat aaagatgata caacagagac tatagaattt
    40741 cctaagtggt tatcaatatt cagagacttc ttaatgggtc tatccatagt tatgttaatc
    40801 ttattttata ttgcaacatt aaaagctggt caagcattta ctcaagaaat tgctggttca
    40861 acacattggt tggtttttcc gcttatagag gcatttacat ttgtggcagg tatgtcaata
    40921 ttgatgtcag gtgttcgtat gttcttagct gaaataactg cagcatttgt aatcatttca
    40981 gaaaagtata ttccaggttc acgtcctgcg ctagatgttc caaccgtatt tccatatgca
    41041 ccaaatgcag ttattattgg gtttatatca gcatatgctg ctggtctatt agcaattgct
    41101 attatggcat catttcctag tatatttcct gtagtaatta ttccagctgc tcacatatgt
    41161 ttcttctcag gaggtacagc tgcaatattt ggcaatactt caggaggatg gagaggggct
    41221 attgctgggt catttgttgt ggggttatta cttgcatttt taccagttat actatatcca
    41281 gtttatggga ctcttggtat agaaggagca actttcccta atattgatta caacgtagtt
    41341 ggttcgatat tacataatat tttacaattt ataaaaccta tttaaactaa attatataaa
    41401 agtatagtca tagttactat gatttcaagg aggatacatc ttatggaaaa tacattatct
    41461 gaatttttag ataatatatc taatgaattg tcagaattta ttgcctcaat agatgagaca
    41521 tctataaata aagcatgcga attaattctt gaagctgaaa aaaatcacgg acgtgtacat
    41581 gtgactggta ttggcaaacc aggacatgta tcgggatata tttcatcatt gctttcatct
    41641 actggaacgt cagcatatat attgcatggt acagaagcag tacatgggtc aagtggtcaa
    41701 gtagttgaag gagatgttgt tattgcaata tcaaatagtg gtgaaactca agagttaaaa
    41761 gcaactctta agactctaaa agtaaatgga gcaaagataa ttggtgtaag tggtaatgag
    41821 agttcatttt taaaaaatat ttctgatata ttcctgtttg caggtgttaa acaggaagga
    41881 gattgtctta ataaagctcc tcgtgcttca attttagctg aaacaataat attacaatca
    41941 ttaagtgttg tattacaata tgctaaaggt cttaatacac agcagtattt gaagtggcat
    42001 ccagcaggaa gtcttggaaa gtctattaga gagggaatat aattttatga aatataaagg
    42061 gattatatgt gcaatgataa caccatttga tgaaaatcaa aatattaatt cacaagcaac
    42121 atgtcaatta attgattatt tgattgaaaa gggaatttat ggattattta ttttgggcac
    42181 taatggtgaa tgtcatgtat taacagatga tgaaaaagtt gaatttgcaa agattgttat
    42241 taatcataca aataatcgtg tacctgtttt tgttggaaca gggggtaatt caactcgtga
    42301 agttgtagat ttatctaaga gaatggaagc gattggagca agtgcattat ctattattac
    42361 accatacttt gttacaccaa cacaacagga attaatctta cattacaaaa ctattgctaa
    42421 ttcagttaat ttacctatta taatgtataa tatgccaggt aagactggaa ttaatattga
    42481 accagagtca gtacgtgaat tatcaaagat aaagaatatt gttggaatta aggatagtag
    42541 tggtaaactt gacaacatga aagcttatat tgaagcaacg aagaatgaga attttagtgt
    42601 gttttcaggc tcagattcac ttattctgga tagtctaaaa gctggtagtc aaggagctgt
    42661 tgcagcaaca acaaacttcc tcacagagat tggtatctct atctatgaaa actttatgaa
    42721 aggggatttg gaagaagctc aaaaagctca aagtagtata gaagaacttc gacgtatact
    42781 gaaacttgga acaattccat ctgttatgaa gaaaactatt gtcttgaatg gaattaatgt
    42841 tggaactgca cgacttcctg taactgaacc aactggggaa gtacttgaag aaattaaacg
    42901 agtagttgaa aattatagga caatacttaa taaaagtagt gagaatagat aatataaata
    42961 gggaaattac actggtatat aattaatata tatattataa aacatgacta aaattaatga
    43021 ataagtaaag tggtttgatg agtatagagg tgataatatg aaaaagatga atataaccaa
    43081 aagaatgtca gaatgtgggg ctcttgcaat tgtaagagaa gaaaatctta accgtgcatg
    43141 tgaaattgct gaaggatgta taaaaggtgg tataacagtt atagaaatga gttataccct
    43201 aaataatgct ggagaaataa ttcaagggct taataaaaag tatggtgaaa cactttgtgt
    43261 gggagcagga acagttttgg atagtgaaac agctcgtcat gctatattac atggagctca
    43321 atttattatt gcaccaaatt acaatgaagg tgtagcgaag atttgcaata gatatcagat
    43381 tccttatgct ccaggatgta catcattgac tgaggcagta gatgcactaa gtttaggagc
    43441 aacttttata aaagctttcc caatatcaga tttctatgga cctaaactag taaaggtatt
    43501 taagacacca atacctgata tgccagtttt agcaagtgga gggattaata ttgaaaacct
    43561 acatatatgg ttagaaaatg gagttgatgt atgtggattt ggtggtttat taactaaagg
    43621 ttctgttgaa gatattgcta aaaatgctaa caagattcgt gaaatcatta aaaatataag
    43681 aaaaatttaa catagggctg tatcatagta tcttataatt actatgatac agccttttta
    43741 atattattca aatttaaaac agtttacatt tctattatat tacatataat ttcagtaatt
    43801 ttatatcaat ttttctatct ggaaaaaaat gatttaaaac ttaagttatg atataatttc
    43861 attaaaagaa ggcgattttt agtaaatatg agaaaataat gtgtttttag gggaaaagtt
    43921 aaatacagat ttaagtgaac aagaatcgat aattaagata ttaaatggtg aatagggcat
    43981 caaaaaaatt actaaattaa aagttttaga gggagaatct tagcaagtgt gtgtgaaaat
    44041 tcaatttcaa acaaaaaata attgtaaatg aaatcaattt atacacgttg aagattcact
    44101 tttatcatta tattaaaaag cagagtgaat taaaagtgat tctaaagata ggaggataat
    44161 taaaacaatg aaagaagaca ttaggttaca tcgaatgtgt aaaaaagaac aagcaacaaa
    44221 gaatgaagtt atatcagagg atattctaaa atctatgagt attggggtca gtaaacattt
    44281 tttagatgat tcatttacaa taatatgggg aaatacatca ttctatcaat tattaggata
    44341 tacagaagaa gaatttttag ttaaattttc tagtttaaaa gattattatg aacagaatct
    44401 ttatcatttt gaatctatga aaaatcattt tttagaggct tatcatcatg gcgaaaagag
    44461 tatagaatat caaatttgca tttcagcaaa taatggagac cctgtttggg taataatgac
    44521 tggtatatta attgaatcac aagataatga gaaattaatg gtctacatat tgtattcaaa
    44581 aattaatgag ttaaaaataa agcaagaaga actatctgat atggaaaaag agagtacaga
    44641 gaattttaaa tggatgatgt cagagtatgc tgggaatgtt tatattagcg atatggatac
    44701 atatgaatta ctatacttaa ataagcactc atgcaatact ttacaggcat cagcaaatga
    44761 acttattgga agaaaatgtt atgaggtaat tcaaggtaga acttcgccat gtccgttttg
    44821 tacaaattcg tatttaaaaa aagatgacac atatgaatgg gaattttata ataaaaaact
    44881 gaaacgaaca tttataatta aagaccgtat gttgaattgg caagggcgga gagttcgtat
    44941 tgaattatct tatgatatgt atagtgctga atataaatta gcaaaaaaag atcaggaaag
    45001 agaatcaatt ttgaaaacta ttccagcagg aatggttaga ttagatgcaa gagattatat
    45061 tactatactt tggtgtaatg atatattttt gaatatgatt gaatatacaa aggaacagtt
    45121 tgcagaggaa cttaataatc aatgtggata tttattttct gaagattata aacgtagtaa
    45181 gaaactggca caagatttga aggagtctgg ggataatgtt gtatttgaag caaaaatata
    45241 tacacgttca agggaagaac gtatatggac tgttacattg tgttatatca gtggggaaga
    45301 cagttgggat ggaataccat ctttttatag tataggtatt gacataacaa aggaaagaaa
    45361 acagattgag aaattacagc atatatcaga gaaagatgca ttaacaggaa tttataatcg
    45421 cgctgagact gaaaggcaga ttaagaaata ttttgaaaaa aatttaaatg ttatgggtgc
    45481 actgtttatg attgatacag ataattttaa acagattaat gatacagaag ggcatatgat
    45541 tggagatata gtacttactg agatggcatc aggtatgaaa aaaataatgc gtgacagtga
    45601 tgtggttgga agaataggtg gagatgagtt tacaattttt atgaagaata tttcctcagt
    45661 taaagatgca gaaaagaaag cagaagaact attgtatatg ttccgtcatc tatttcagaa
    45721 agaaaaaagt tttctaaaag taacttgtag cataggaata gctatatatc caaaggatgg
    45781 tactacattc aaggaaatat atgcaagagc tgataaagcc ctgtatcaag caaaaaatat
    45841 gggaaaaaat aattatgtaa tctacaacca agattatttt aaagaattag aagaatttga
    45901 ttgttcttct cttggtacag ttattgattc agaaaaacaa tatgctgaat gtcctgataa
    45961 tcttacacgt tatatattcc gtatgcttta tcagactgat gatatagacc aggcgattaa
    46021 tatgatactt gaggttgttg ggaaacagtt tgatgtaagt cgtgtttata tttttgaaaa
    46081 tacagaagat ggtagatata cttctaatac ttatgaatgg tgtaatgaag gaatctcttc
    46141 tcaaatggct tatttgcaaa acagcaatta tcaagaatat gaggattatg aaaagctttt
    46201 tgaggatgaa ttagtttttt attgtcgaga tatccataca ttaagtccta aactagaaga
    46261 attgttttct agtcaaggta tccactctac acttcaatgt gcatataaag aagatagagt
    46321 tttcagtgga tttgttggat ttgatgaatg cacaggacta cgtctatggt cacaagagga
    46381 aataagtaca ttatcattga tttctcaaat tatatcaatg tttttacaac gtaaaaaaaa
    46441 tgataaactt aatcaacaga ttcaacaata ccagaatatt ttagataata ttaaccagtg
    46501 tatttgtgtg attaataaag aaaataattt tttattatat gtaaataaaa aatttaaaaa
    46561 tacatattct aaattccaaa ttggacaacc ttattatctt gattctatag atattcataa
    46621 gatatccatt atatggaata aaaaagaagc atacctatgt actagcacga tttaatggta
    46681 aagtaaagtt tatattggac ttaaattaaa tttcgtttta aataagatat tattttagtt
    46741 gcttactgaa aagactagaa tctatattaa tgaattatac taattttata ccactattaa
    46801 aatattgttt gctaaaaata aaaaatagcc ttaaaacagt tgattataaa aaattaactg
    46861 ctttaaggct atttttatct ttgtgacgaa atagacactt gccatttaga atgtgacaat
    46921 atagtagttg tttagaaaag gcacaaagca ttatacttta actaaagtaa aatgtatttt
    46981 gcttttagaa taaaaacaat taggagggag ctataattga aaaagataat atatgtatta
    47041 tttacaattg aactattaat agtatcttta ttaggtctaa atatagttaa agataatgaa
    47101 attaataata tttt
//
LOCUS       DAEMHW010000007        46296 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEMHW010000007 DAEMHW010000000
VERSION     DAEMHW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.007"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            137..436
                     /locus_tag="KPK67_000299"
     CDS             137..436
                     /locus_tag="KPK67_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457317.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKTETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            760..2415
                     /gene="ilvD"
                     /locus_tag="KPK67_000300"
     CDS             760..2415
                     /gene="ilvD"
                     /locus_tag="KPK67_000300"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBG2457318.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRMAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2759..3391
                     /locus_tag="KPK67_000301"
     CDS             2759..3391
                     /locus_tag="KPK67_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2457319.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSVNDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFRQL"
     gene            3414..4226
                     /locus_tag="KPK67_000302"
     CDS             3414..4226
                     /locus_tag="KPK67_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2457320.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
     gene            5309..5668
                     /locus_tag="KPK67_000303"
     CDS             5309..5668
                     /locus_tag="KPK67_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2457321.1"
                     /translation="MRKKKDTHSFDFRPLGLAIREAREKAGLSRNDLGDKVFYGERHI
                     ADIENIGKHPSFHLFHDLVTMFNISVDEYFYPAKNVEKSTVRRQIDSSLDLLSDDELK
                     IIQGTIDGILNSRENKK"
     gene            complement(5766..5966)
                     /locus_tag="KPK67_000304"
                     /pseudo
     CDS             complement(5766..5966)
                     /locus_tag="KPK67_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6079..7401)
                     /locus_tag="KPK67_000305"
     CDS             complement(6079..7401)
                     /locus_tag="KPK67_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2457322.1"
                     /translation="MNQKNENYWLLDAPVTKAIWHMAIPMMLGMSINIIYNITDTFFI
                     GRLNDTAALAAISLLLPFTTILMAIGNLFGTGGSTLFSRLLGSENTDRTKQCSATTLW
                     LSFLFGLLTAIISIIFSNYIIRLLGADSNTFAYVKQYLIFYGMGAPFIIANFTLEQLI
                     RGDGKSVESMIGMMISVGANIILDPILMFGLQLGIRGAAIATVIGNAFAVIYYIVCIQ
                     RADNQLSALPKYFRLEKQMLKEIFLVGLSAMLLDILLIVSSLMFNYYALKYGDYVLAG
                     FGISQKLVQIVDLIGMGLYMGVIPLIAVAYGARNELRMKEIIKKTALYLALVITCLFA
                     ILFTCRNFIVHCFSNDSDVIRIGAYILTVQLCSSFFAAGAGLLTGIFQAKGEGTPAIV
                     MSVMRGLMLIPAIIFGNYLFKMNGVIFSLLVAEAISCITGLVLYKLKK"
     gene            complement(7490..8143)
                     /locus_tag="KPK67_000306"
     CDS             complement(7490..8143)
                     /locus_tag="KPK67_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2457323.1"
                     /translation="MNNIYSDIYEKLSTLQWLMKRHQMFCQAESGPFADTSRGQGRIL
                     AMLKIQPEIATKELAYLLGIRQQSLNELLNKMEKNGYVERKPSENDKRVMIVHLTEKG
                     KQVQQPKTDYQNIFNCLLPEELLQMSKYLDRIIEAFQLQNGNALEENNMIDWMAQARE
                     RMGDEHFEQLMSMRERAFGHMRPPKDIPGAERFSENYNGYVPDRDGFQPRNFRPDIK"
     gene            complement(8280..8691)
                     /locus_tag="KPK67_000307"
                     /pseudo
     CDS             complement(8280..8691)
                     /locus_tag="KPK67_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8690..9791)
                     /locus_tag="KPK67_000308"
                     /pseudo
     CDS             complement(8690..9791)
                     /locus_tag="KPK67_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
     gene            10324..11262
                     /locus_tag="KPK67_000309"
     CDS             10324..11262
                     /locus_tag="KPK67_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG2457324.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
     gene            11738..12166
                     /locus_tag="KPK67_000310"
     CDS             11738..12166
                     /locus_tag="KPK67_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG2457325.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECVELGKLLAE"
     gene            complement(12318..12516)
                     /locus_tag="KPK67_000311"
                     /pseudo
     CDS             complement(12318..12516)
                     /locus_tag="KPK67_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12863..15517
                     /locus_tag="KPK67_000312"
     CDS             12863..15517
                     /locus_tag="KPK67_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBG2457326.1"
                     /translation="MIKIMKRLLTGVLTLATVFTALPTTAVHAAQTQYWTESAERVGI
                     VEKVMNDGSIGSTFNEGHMTVEGEDAYCIDINTNFKNGYKTRADASTRMSADQIADVA
                     LSIEYVKQYTDSHKGISSQHAYLLRQLVVWQRLSVHLGWGCDNVRAAYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCYGYIYTGEGQDIGQFYAKLAVGNGKIQKSSSNKGITESND
                     CYSIAGATYGVYADKSCKEQLATLTTDNSGSTETVELKAATYYVKETKAPKGFQLDKN
                     VYTLTVKVGETAILKVSDTPKVTDTLIELFKIDMETSKADPQGNASLEGAEFIWKYYD
                     GYYTKDNLPAEPTRTWTTKTKAEKGSDNTIHYITRLADSYKVSGDSFYTQNGTVCLPL
                     GTITVEEKAAPNGYLLEGAYMQAAGSSEQIKGLYVAQITENGELAALSGSNQYSVSDK
                     VIRGKIQKRDLETKDTKAQGGATLKDTAFAIISLNDNAVLVDGKLYKKNETVKTIHTG
                     IDGIAATDTDTLPFGKYRLEETSAPEGYLTDSAKAVEFEITENGKIVDLTDEEHSIYN
                     QIKRGDIEGVKIAAGTHKRLANVPFRITSKTTGESHVIVTDANGQFSTSADWVSHTQN
                     TNEGKTSEDGIWFGTSAPDDSKGALPYDTYTIEELRCDSNKGMTLIPDFDVVVSRDKT
                     VIDLGTLTDEYEPEINIHTTAADKATGEKSIVAGKSVTIVDTVTLDGLKKGTKYQLKG
                     WQMIKSENAQLLVDGKPVENDYTFTATDSKMEVQIEFTFNASELAGKELVTFEELYDV
                     TNPDEPIKVAEHKDIEDEGQTVTVKEQPESPVTPEESDTPETPSRAGSSPKTGDNTPL
                     TALLAMLGISSAGLIFAAYKRFYKKKAD"
     gene            15753..16061
                     /locus_tag="KPK67_000313"
                     /pseudo
     CDS             15753..16061
                     /locus_tag="KPK67_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
     gene            16083..16448
                     /locus_tag="KPK67_000314"
     CDS             16083..16448
                     /locus_tag="KPK67_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005363242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG2457327.1"
                     /translation="MRLANGIIIDKEKTFGVLKFSALRREVHIQNEDGTVSEEIKERT
                     YDLKCKEQGRMIQVSIPATVPLKEFNYNAEVEIINPVADTVATATFQGADVDWYIKAD
                     DIVLKNKVTTLNGSQNKNK"
     gene            16522..17313
                     /locus_tag="KPK67_000315"
     CDS             16522..17313
                     /locus_tag="KPK67_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HBG2457328.1"
                     /translation="MINYSILKIKLLSIIESIDDYTSEPIISVPCTTLKNDLENDTMT
                     IDVLEYCCNIIEDWYKDNLSHICSNQYVIRPEKHKKNTQTIKEINEDIQVNREEYEKY
                     FSNPSISQANPSISGDKIFIVHGHDNGLKNEVARFLEKLKLKPIILHEQASEGETIIE
                     KIERYSNVGFGIVLYTPCDKGGLANDGAELRCRARQNVVFEHGYLIGKLERKNVCALV
                     KDDVEKPNDISGVVYVSYDESGAWKIEIAKELKAAGYNIDFNKLF"
     gene            17381..18178
                     /locus_tag="KPK67_000316"
                     /pseudo
     CDS             17381..18178
                     /locus_tag="KPK67_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203305.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            18523..18741
                     /locus_tag="KPK67_000317"
     CDS             18523..18741
                     /locus_tag="KPK67_000317"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457329.1"
                     /translation="MTQTVSLLWVSKTRFYENPYNVTGIIKVAGLEKKPTDICTDSVL
                     GTLKAGNMEILLSMKCSHHKVIVRLAMI"
     gene            18909..20729
                     /gene="ltrA"
                     /locus_tag="KPK67_000318"
     CDS             18909..20729
                     /gene="ltrA"
                     /locus_tag="KPK67_000318"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBG2457330.1"
                     /translation="MLKKDKLRYNEYYDMQHIYDELYARSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKTNSGSKTAGTDGLTIDDIKHLCDEDIIMKVRSSLDNYQPKSVRRVFIPK
                     SGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHALGRVTSL
                     INISKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKHRNKNGWFQYAKKHTRLKSGFI
                     VRYADDFKIMCSTYEEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGKTKHGYVAKTDMNQKALKKAKTNLKLKVKDIVRHTTTFQIARYNLAVMGMQN
                     YYCVATNIYNNLTEVSYALLPTTRVRFKKIAKLIPFETTSQDFQMKTTGIRPQTKIIM
                     IADTPLLPINGVKHKNPLNFSQDICNFTEHGRSRIHKEIALVTKDEIRILLEYKDNTK
                     SVEFNDNRISVFIAQQGNCYITNRRHSPTDMVCIYKNITETDRDKYQNLVFVEIPISK
                     AILTESVEQAKMWLMNYGLNSQQKKKLNKIRANYGYQTIK"
     gene            20845..21450
                     /locus_tag="KPK67_000319"
                     /pseudo
     CDS             20845..21450
                     /locus_tag="KPK67_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            21743..22942
                     /locus_tag="KPK67_000320"
     CDS             21743..22942
                     /locus_tag="KPK67_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG2457331.1"
                     /translation="MNETDFLTALKEKRCAYGVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNPNNPMFLLFDYVRIRFPTMDIKHIIKDILKLNIGYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLLAQERSWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIRKCENEECISKFRSFKSYGSGELVKHNETDKYG
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAELTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTQPEPY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHYQIIKQQTATVEE
                     MVANVKE"
     gene            22950..23084
                     /locus_tag="KPK67_000321"
     CDS             22950..23084
                     /locus_tag="KPK67_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG2457332.1"
                     /translation="MWELLKDLLLVSFGMGIGVVLMCILTVGKEADRCMEEIKEREDN
                     "
     gene            23085..23306
                     /locus_tag="KPK67_000322"
     CDS             23085..23306
                     /locus_tag="KPK67_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457333.1"
                     /translation="MNFGQNLYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALVAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            23390..23857
                     /locus_tag="KPK67_000323"
     CDS             23390..23857
                     /locus_tag="KPK67_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG2457334.1"
                     /translation="MRNLDINVLHLDELQIFGLWGKSNDKTISNDIKILSKKYHSMVS
                     LPEGKVFPYFVLARNYDEQSKDFEMFIGSTIDKNELESLILSAGEYAKITVKPKLGFL
                     WGVSVGEAKRYFYTKWLPESEFKALNMEYEYHTEKSIGKHPTIDIFFAIERKS"
     gene            23912..24361
                     /locus_tag="KPK67_000324"
     CDS             23912..24361
                     /locus_tag="KPK67_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457335.1"
                     /translation="MSYEAQIEMLTCSDNKQAYNALKELLLVCEHSNDLYIFFDKFVD
                     MMNNKTNSFIRTRGLRLIAYHAKWDNENKINSIIEQWLSHIEDEKPITARQCIQDVIV
                     IAKYKPELINVILDALGHFDQIYDDSMQKLVYKDRQKAIKIIRQYTW"
     gene            24458..24754
                     /locus_tag="KPK67_000325"
     CDS             24458..24754
                     /locus_tag="KPK67_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2457336.1"
                     /translation="MNDIFRDMQAKIGCENISDLPSYKRKVWHEMKQLNIADYEEKQL
                     EDFSKYVFGMSCQTLKDVIKQQKGREEQCRKQGCWWKRAEQLAKKQRHTGSTCR"
     gene            24673..25176
                     /locus_tag="KPK67_000326"
     CDS             24673..25176
                     /locus_tag="KPK67_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2457337.1"
                     /translation="MQETRVLVETSGTTGEETTSYWFDLPIEVAEFEEMLGVGAESGE
                     YRIIEKALPFADEVHEHTSVYQLNELDYMYRQLSSDMQEEYTMLHTVYENLEALYICR
                     NVITLYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFETYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            25194..25697
                     /locus_tag="KPK67_000327"
     CDS             25194..25697
                     /locus_tag="KPK67_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2457338.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIEIGEYVSIEELNEMYEMIEELPNYIVDCLDEFIGHYGTLEEVVEN
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIATSR
                     GMCEIPY"
     gene            25806..26195
                     /locus_tag="KPK67_000328"
     CDS             25806..26195
                     /locus_tag="KPK67_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2457339.1"
                     /translation="MKKIRSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPAFLTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLQKEIFNETITAVRSVNYVPD"
     gene            26182..28632
                     /locus_tag="KPK67_000329"
     CDS             26182..28632
                     /locus_tag="KPK67_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2457340.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKRLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNLKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLTEHLYGRDGIAYEDYEYQLPKKKLKKE
                     TLIKYYDLIRPTRCVVEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVIRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSETRGLLHVIDELRREDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            28632..30842
                     /locus_tag="KPK67_000330"
     CDS             28632..30842
                     /locus_tag="KPK67_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG2457341.1"
                     /translation="MKERIQSAFTKKKMFRFLKITVFVVALSLILLSLLGTVAHATGL
                     VDDTINTENLYSKYPLSNYQLDFYVDNSWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTSSGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETNKALHAVINFVVVFILSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIENNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVANKRAERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQHRKNIADKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATALTVSKTGAEKMQEIKRPATATTSKASEPVKTNVIKERPLSSGTSDRKATQSTQP
                     VHRQNVEKVVSQETRQNYKAVRTTKTQTFEQSKRTTEHTEKNRNLVTKKGQKKK"
     gene            30839..31789
                     /locus_tag="KPK67_000331"
     CDS             30839..31789
                     /locus_tag="KPK67_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG2457342.1"
                     /translation="MKLKHIALISSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSVKQGCKYFASLLSSCKNQGIDDLNVAIQSYNYGGGYVGYVAGKGKKHTYNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYQYGNQFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSSPTTSFDCSGLTQWCYGKAGISLPRTAQMQYDATQHLPLSQA
                     KAGDLIFFHSTYNAGSYVTHVGIYVGNNQMYHAGDRARRS"
     gene            32514..34328
                     /gene="ltrA"
                     /locus_tag="KPK67_000332"
     CDS             32514..34328
                     /gene="ltrA"
                     /locus_tag="KPK67_000332"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBG2457343.1"
                     /translation="MTTKTQKKKALRFNEYYDTQDIQDKLYQLGKENYKFTNLVELIT
                     DKRNVMLAYRTIKTNKGSKTAGTNSNTITELAETSPELLTEYVKIRLKNYIPHAVKRV
                     EIPKPNGKTRPLGIPTIEDRLIQQCIRQILEPILEAKFYEHSYGFRPDKSTSHAIARF
                     YHLAHSGFHYVVDIDIKGFFDNVNHGKLLKQLWTLGIRDKNLISIISRMLKAPVKNNG
                     VPNKGTPQGGILSPLLANVVLNELDWWIASQWDNHPTNYPYKHQRSKLLALKETQLKE
                     IKIVRYADDFKIMCKDCDTAQKIFMATKKWLNERLHLEISPEKSKITNIRKNYSEFLG
                     LKLKVKKGKAGKYTNRSHMRDKAKKLAVEKLKEQIKTIQKNPTREAVNKYNSTVLGLQ
                     NYYSMSTMVNYDFVEIAYIVNKSLLCRTKHIRSKNGTITQTYQKFYKEYGYKKIFIAG
                     TALFPIAGIKFREPKMISKECTRYTVEGRNAIHKRLQMNMRIIHYLMEQTSPLQGTTE
                     FNDNRISVYVAQRGKCKITGKELKIGNMDLHHIKPKFKGGSDEYKNLIYVTNEIHILI
                     HSSAKETTEKYLKIVKPDKAQLNKINQYRVMVGNNELD"
     gene            34521..35423
                     /locus_tag="KPK67_000333"
     CDS             34521..35423
                     /locus_tag="KPK67_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2457344.1"
                     /translation="MFKKNKKQTENIKEKKVRTVKIGTHKKTVIALWVVLIASVSFGI
                     YKNFTAIDMHTVHETETIQLRLTDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAISG
                     YLTKELQDLNVDTIRTDIPTSSTVTDVIVWNIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVYVDVDGDMVIIQNPTLAPTIEKSDYEPKTPEAGASVDADTINDATSFLE
                     TFFKLYPTATEKELAYYVSDNVLEPIGRDYLYSELINPVFTKDGGNVKVKVAVKFLDN
                     QTKATLVSQYELVLYKDSNWKIIM"
     gene            35636..36493
                     /locus_tag="KPK67_000334"
     CDS             35636..36493
                     /locus_tag="KPK67_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2457345.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGVHYLSHGKSPKIKKNYTMEDMLHNVYDATTYGIENFGENIAIMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETLSLTKYPAWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDKVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLTLELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            36571..37152
                     /locus_tag="KPK67_000335"
     CDS             36571..37152
                     /locus_tag="KPK67_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2457346.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            37256..38101
                     /locus_tag="KPK67_000336"
     CDS             37256..38101
                     /locus_tag="KPK67_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2457347.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            38098..38781
                     /locus_tag="KPK67_000337"
     CDS             38098..38781
                     /locus_tag="KPK67_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2457348.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKISEYILSKTVALIAISTV
                     IALILGVVSGSNHLLGITVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPYLFRFFPYTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVKHM
                     WKSLGGVKL"
     gene            38778..39476
                     /locus_tag="KPK67_000338"
     CDS             38778..39476
                     /locus_tag="KPK67_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2457349.1"
                     /translation="MKRILSSTIQVFRQIKSDPMMFAACFTPFVMGALIKFGIPFLER
                     ITDFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLEG"
     gene            39488..40399
                     /locus_tag="KPK67_000339"
     CDS             39488..40399
                     /locus_tag="KPK67_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG2457350.1"
                     /translation="MEKGRLLQNGKVNRAIQVSKITLFINLSLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDREHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSEQYNTLSAPSGIALTAAVLSIVIKEWMYWFTRNAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            40451..40591
                     /locus_tag="KPK67_000340"
     CDS             40451..40591
                     /locus_tag="KPK67_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457351.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNSPKDSEDDK
                     MY"
     gene            40881..41507
                     /locus_tag="KPK67_000341"
     CDS             40881..41507
                     /locus_tag="KPK67_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2457352.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKEYQVPESTIVVTPNAYRNTTIESLTTFTFAQYDFW
                     VTDEFASNFRKMLILEQYRNLKMNVLYQKCLVNGPISYIEDIFNEMIKKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKSQLTTHVNQFVKKYITNEKVK"
     gene            41509..41595
                     /locus_tag="KPK67_000342"
                     /pseudo
     CDS             41509..41595
                     /locus_tag="KPK67_000342"
                     /inference="COORDINATES: protein motif:HMM:NF025568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            41629..42345
                     /locus_tag="KPK67_000343"
     CDS             41629..42345
                     /locus_tag="KPK67_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2457353.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            42508..42699
                     /locus_tag="KPK67_000344"
     CDS             42508..42699
                     /locus_tag="KPK67_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBG2457354.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            42723..42953
                     /locus_tag="KPK67_000345"
     CDS             42723..42953
                     /locus_tag="KPK67_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457355.1"
                     /translation="MIGLKRRNMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            43220..43639
                     /locus_tag="KPK67_000346"
     CDS             43220..43639
                     /locus_tag="KPK67_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2457356.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            43642..43896
                     /locus_tag="KPK67_000347"
     CDS             43642..43896
                     /locus_tag="KPK67_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2457357.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLVFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIHIDIETEEE"
     gene            44269..44472
                     /locus_tag="KPK67_000348"
     CDS             44269..44472
                     /locus_tag="KPK67_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG2457358.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRRLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            44550..45740
                     /locus_tag="KPK67_000349"
     CDS             44550..45740
                     /locus_tag="KPK67_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2457359.1"
                     /translation="MSKVKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRN
                     TKIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFNFKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYHDEVI
                     LLLETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISTPKTKNGKRELPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     RLAS"
     gene            45891..46211
                     /locus_tag="KPK67_000350"
     CDS             45891..46211
                     /locus_tag="KPK67_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBG2457360.1"
                     /translation="MIRNLVSFFIKKLSETDEFFRVFGSLDDKTYSEGAISKKNKELM
                     GLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAIKILK
                     EFNI"
BASE COUNT        15792 a         7652 c         9538 g        13314 t
ORIGIN      
        1 tattaagatt aaaaatatat ataatcaaag tttctacaat tatatagtta ggtatataaa
       61 aattttatcg tatgcataaa tattataaat atatggaaat aataatagta tattttggta
      121 aagtaggagg aattttatga aatggattaa atggtatagt attacatgca tatgtatttt
      181 tatagtagta gctttttata tgtttatatt tcctaataaa acagaaacta ttgatacttc
      241 atctgcatat agctttgttg aaaagaaagt accaaatagt gctgtgtatc aaggatacaa
      301 aaagaatcca gttgatggta ctactacaat ttattacagc tatgataatt caactcatat
      361 agttagatta tcacatccag aagactattc aaggaagatt aattgggata aagtttcaaa
      421 catcagattc gattaatctt taatatttat aaaagtctat atatctaagg caaatatatt
      481 attaatctta ttcatagttg agcactttct attatagaag tgcctcttct gatttatatc
      541 ataaaatatt actcaacaat ttgtaaaata tattagttta taaaatatat tgaaaagata
      601 atattatata atacgattgt gtaagatatt gataaaaaat cgtaataaat tttcattagt
      661 atatgctaat atttttttac aattttcgta attaattaat agaaaaatat attataataa
      721 tattatgaaa atttaaaaaa gtaagaaaga ggtaatatta tgagaagtga tataaaaaaa
      781 ggaatagaag gagctccaaa gagagcgtta atgtatggaa tgggtcttac taaagaagaa
      841 attgaaagac cattaatagg tatagtaaat gcacaaaatg aggtgattcc tggtcatctg
      901 catcttgatg aaatagcaga agctgcaaaa aatggagtaa gaatgagtgg tggtttgcca
      961 ttagaatttc ctgctatagg ggtatgtgat ggtatagcta tggggcatgt cggaatgaat
     1021 tattccctgg cttcaagaga attaatagca gattcaatag aagctatggc tatggcacat
     1081 ggttttgatg ctttggtact tatacctaat tgtgataaga tagtgccagg tatgttaatg
     1141 gctgctgcaa gacttaatat accaagcatt gttgtgagtg gagggcctat gcttccaggt
     1201 aaaaaaaatg gaaaagtata cgattttaac tctgcaatgg agggtgttgg agcatgtaaa
     1261 gatggaacag taagtgaaga agaattagaa gcgttagcca tgaactcttg tcctggatgt
     1321 ggttcatgtt ctggtctttt cacagcaaat agcatgaatt gtttaacaga agctttaggc
     1381 atgggtatac catataatgg tactgctgcg tcacattctg gagaaagaaa aaggatggca
     1441 aaatatgcag gtatgtatgt tatggagtta cttaagaacg acataaaacc tagagatatt
     1501 ttaacaatag atgcttttaa aaatgctata gctgttgata tggcaatggc tggttctaca
     1561 aatacagtac ttcacttacc tgcaatagct tatgaatcag gaatagagct taacttagat
     1621 ttttttgatg aaataagtga aaaaactcct tgtttaacaa aattaagtcc aagtggaaaa
     1681 catcatattg aagatttaca tatggcagga ggaataccag ctataatgaa cgaactttca
     1741 aagataaatg gaataaattt agattgcaaa accgtaacag gcaagactat aggggaaaat
     1801 ataagaaatt gtgaaataga aaatgaagaa gtaatacata cattaaagaa tccatatagt
     1861 aaccaaggtg ggcttgcaat attgaaagga aatcttgctc taaatggagc tgttgtaaaa
     1921 aagtcagcag ttgcagaaga aatgttagtt catgaaggac cagcaagagt ttttaattca
     1981 gaagaagaag ctgtaaatgc tatttttggt aaaaaaataa acaaaggtga tgttatagtt
     2041 ataagatatg aaggtccaaa gggtggtcca ggaatgaaag aaatgctatc tcctacatca
     2101 gcagttgcag gaatgggact tgataagcat gtagcacttc ttactgatgg tcgtttttca
     2161 ggggcaacta gaggagcatc tataggccat atttctccag aagctatgga aggtggtttg
     2221 atcggactag ttgaagaagg agatataatt tctataaaca taccagacaa aaaattagag
     2281 ttaaaagtag atgaagttga aatagaaaat agaaaattaa aatttaaacc tttagaaccg
     2341 aaaataaagc atggatactt aagtagatat gctaaactgg taacatcagc aaatacagga
     2401 gcagttttaa aatagtagac tatatttatg taaattagaa aaagttaaga aaatattaat
     2461 tggatatctc aggtgttata gcactaatga ggtatccttt ttattttgta tgaaaaacac
     2521 agcaaaatct tgattatctt tgaaaacaat acatctctat tataaggaat acgagtacag
     2581 catagagatg tatcttttag accttgttga ggtaatacaa ctttttaaaa gtttgatgtg
     2641 gaatgctttt cgttccatct gaactatgaa gaaattgaaa gtaagttaat tcttgacata
     2701 ataaatatta aaatgtataa taaaaaacaa acagacagtc tgtttttagg agggaaatat
     2761 gaatagagaa gaaaaaagta aaaatagtaa agaaaaaatt atccaatcag cattttcact
     2821 attttcgtct aaaggatatg attcaacatc tacacaagat attatcaatt tatctggtct
     2881 atctagaggt gcaatgtatc atcactttaa aactaaagaa gatatactga gaagtgtcac
     2941 aaaagaactt tactcacaaa tgaataattt tttagagcat cttgttgctg atgacaccct
     3001 tacagcaaat gaaaaaataa tagaattggt tgttcatagt gtgaatgatt acacacgtag
     3061 aaaaatggta cattgtagct ggttagaaaa aatcccattc gctttaatag aggaagttcg
     3121 taatctcaac aatgtagttg cacccaatat tgctaagata attaaacaag gtgttgaaaa
     3181 taaagaattt tcttgtgaat atccagagga attagctgaa atgctcgttt ttagtattga
     3241 tattttactt gaccctgtat tatttaaacg tgaatacagt gaagtatgta ataggttgga
     3301 ttttttatta ttcatgttaa aaaaaatgga tattcctcta attgatgagt acggtattca
     3361 aaaatttaaa gatttattta gacaattata ataggaaaag cgaggaatag actatgtttt
     3421 ataatgcaaa aagttgtcaa atgatgatag gaagtaatac agtgaattat attgaatttg
     3481 gtagtggtaa gaagacacta attattttac caggattagg agatggttta tttcctttac
     3541 atggaaaaat acaggcgatt gcttttgctt ttagatataa gcaatttgca aaagattata
     3601 aggtttatgt atttagtaga aaaaatcaaa ttacagaaaa atattccaca agggatatgg
     3661 ctaaagacca agcagatata atgaaaaaac ttggaattat gaaagcagaa gtaatgggtg
     3721 tttctcaagg tggaatgata gctcaatatc tggcaattga ttatcctgag ttagttgaga
     3781 aacttgtgtt ggcagttact tcttcaaagc aaaatgatac tatccaaaat gttatttgta
     3841 gttggattga tatggcgaag aagcagaatt acaatgattt aatgattgac acagcaaaga
     3901 aatcctattc tgaaagatat ttaaaaaaat accaattatt tattcctttt ttaggaaaag
     3961 taggtaaacc aaaggatttt aaacgattta ttattcaagc gacctcttgc atagaacata
     4021 atgccttttc cgaattaaat aaaataacat gtccaacctt aattatagga ggtgctaatg
     4081 ataaaattgt tggaaataat gcttcttttc atttagcaga aaaaattaaa aaaagcgaaa
     4141 tatttatcta tgagggattg ggacacgcta catatgaaga agcacaggat tttaatgaaa
     4201 gagttttaga gtttttaaat aagtaaacac ctttgttaaa aatatacata tgaagattgg
     4261 aaatttaatg ttaaaaatag aaacatgaaa atatgctcaa ctggtatttt tactattatt
     4321 tttactattc ataaccaatt tttgatacat tatctactat aaatacaaat atagcttcaa
     4381 tgtgattata tattggtgta ttggtaaagc acttatacaa acagaggaat tttgtaaatt
     4441 cagattatat ccacatttgt taacttatga aaatataatc aaaattttta tgagattttt
     4501 atataaaaaa tacactaaat ttctgattat cttttcatag gttggtaaat gccgatatat
     4561 cctgtatttt tcaagttctt catcaagcac acggatttcc gtactgcaca catcgaatgt
     4621 cgtatagtca ctttcgtaat catcccagac aatcaatttc tcaataacaa tttctggaag
     4681 ctcacaaaag ggtacttcct ttgcctgtca tctggctaat tccttgcagt agttacagac
     4741 actgcctttc acaacctttt tgagcttaca gtccaactga cactgtatcg tcttttggaa
     4801 gtctgatggt ttcaaaatca cacccccttt cgctatgaag tgaaaagtat gtatccctct
     4861 ttccgcacca tatctgtaca gcagggtgtg atttgttgca gaattgaaaa actttttgaa
     4921 aatatgaaaa cagcctatct gcactaataa caaatagact gtatttaaaa catctgatat
     4981 atgagttttt aattcatgct caagggctga tgataaacta aaaaaaaggg aataaattcg
     5041 gttgtcggtg ccggctgcct gttttactta gaatcacaaa ttgctccttg agcagagcaa
     5101 acctgtgaaa atagaaaaag gacagctctg gttatcccaa aactgtccta atatttttgg
     5161 acactataaa agagattacc tacagaacgt tttttttatt taccgaacga ggtaatattt
     5221 tgcttgaatt tttagtgcat ataatataag tttttaacac attctaatca cataacatga
     5281 agtataaact catgctaggt gattaaaaat gagaaaaaag aaagatacgc acagctttga
     5341 ttttcgtcct ttaggactgg caatcagaga agccagagag aaagcagggc tttccagaaa
     5401 tgatttaggc gataaagtct tttacggaga acgtcatatc gcagatattg aaaatatcgg
     5461 gaaacaccct agctttcatt tattccatga tttagttacc atgttcaata tatctgttga
     5521 cgagtatttc tatccagcga agaatgtgga aaaaagcaca gttcgccgtc agatagattc
     5581 gtcacttgat ttattgagtg atgacgaatt aaaaatcatt caaggtacga ttgatggtat
     5641 cttgaacagc agagagaata agaagtgatt cgcttcttat tttctctgtt tttctgtgca
     5701 gattcttcaa cgcttatttg ttgatatact ccacaggtaa caaatagtcg taaatcaaaa
     5761 ttttgttttt ttgtaatctt gtaaatatat tcttctggtg taggacgttt attgccaaaa
     5821 tactttttga aatttgcctg tacctctggg gctggactgt tcatttcttc atcaatagtg
     5881 gcttctatat tagcccgaat atcagctaat gatacatcta ccagttttca acattttttt
     5941 atatttcttt ttttaaaccc aatcatattt ttccagctcc taccaatcta tactcattct
     6001 atagaaatct cttaatttat tgttgatgat ttttataacc atcactaact ttgttaacaa
     6061 gtctgatgga gttcagcatt actttttcaa tttatataat acaagccctg taatgcatga
     6121 aatagcttcg gctacaagca gagaaaaaat aactccattc attttaaaga gataattgcc
     6181 gaagataatt gctggtatga gcataagacc tctcataacg gacataacaa tagccggagt
     6241 tccttctcct tttgcctgaa aaattcccgt tagaagaccc gctcctgccg caaagaaaga
     6301 agaacagagt tgaacggtta agatgtacgc acctatacga attacatctg aatcatttga
     6361 aaaacaatga acaataaagt ttctgcatgt aaatagaata gcaaacaaac atgtaattac
     6421 tagtgctaaa tagagagcag tctttttaat gatttctttc atacgaagtt catttcttgc
     6481 accataggca acagcgataa gtggaattac tcccatgtaa agtcccatgc cgattaggtc
     6541 gacaatctgc acaagttttt gagaaattcc aaatccggca agcacataat ctccatattt
     6601 tagtgcatag taattaaaca taaggcttga aacaatcaaa agaatatcta acaacattgc
     6661 agataatcca actaaaaaaa tttcttttag catttgtttt tcaagcctga aatattttgg
     6721 aagagcagat aactgattgt ctgctcgttg tatacagaca atataataga taacagcgaa
     6781 agcatttccg attactgtag caatggctgc cccacgaata ccaagctgta atccaaacat
     6841 cagaattggg tcaagaatga tattagcacc aacgcttatc atcattccaa tcatagattc
     6901 tacagattta ccgtcacctc taattaactg ttctagcgta aaattggcaa taataaacgg
     6961 agcacccatt ccataaaaaa taagatactg tttgacatag gcaaaagtat tactatcagc
     7021 accgagaagt cggataatat agttactgaa aatgatggat attattgcag tcaatagtcc
     7081 aaacaggaaa gatagccata atgttgtagc tgaacattgt tttgtcctgt ccgtattttc
     7141 acttcctaac agtcgtgaaa acaaagtgct tccacctgtt ccaaacaaat tcccaattgc
     7201 cattaaaatg gtcgtgaaag gtaacagcag tgagattgcg gcaagagccg ctgtgtcatt
     7261 taatctgcct atgaagaatg tatctgtaat attgtaaata atgtttattg acattccaag
     7321 catcattgga atcgccatat gccatatagc ttttgtgaca ggtgcgtcta aaagccagta
     7381 attttcattt ttctgattca ttttgttcgt ctccttttca aaaaataagc cctatggagt
     7441 tcaaaaaatc tccatagggc ttattgctgt aattttacag tgccttttgt tatttgatat
     7501 ctggtctaaa gtttcttggc tgaaatccgt ctctatctgg aacatatccg ttatagtttt
     7561 cagaaaagcg ttcagctccc ggtatatctt tgggtggtct catatgtcca aaagctcttt
     7621 cacgcataga cattaactgt tcaaaatgct catcgcccat acgttctctt gcctgagcca
     7681 tccagtcaat catattgttt tcttctaaag catttccatt ttgcaattga aaagcttcaa
     7741 taatacgatc taaatatttc gacatttgta gcaattcttc tggaagtaga caattaaaga
     7801 tattttgata gtctgttttc ggttgctgaa cttgctttcc cttttctgtt aaatgaacaa
     7861 tcataacgcg tttatcattt tcagatggtt ttcgttctac atagccgttt ttctccattt
     7921 tattaagcaa ctcatttagg gattgttggc gtattcccaa taaatatgcc aactcttttg
     7981 ttgcaatttc tggctgaatt tttaacatag caagaattct tccttgtcct cgtgatgtat
     8041 cagcaaatgg accagactct gcctgacaaa acatctgatg gcgtttcata agccattgca
     8101 aggttgatag tttctcatag atatcagaat aaatattatt catagtatga cctccttttc
     8161 aacaggtacc tttaacaatt aatatacagg taccttccca aaaagtcaat agggtacatg
     8221 tatattttta aaacttagta tatgtatttg aaattgccat agctggtctg ctgtaatatt
     8281 tatcctacaa ttttccaatt atcgtccttg tgaagcacaa gctcatactg tgagatttgt
     8341 gtcattttcg acttgttatc cagatatttc acggacacac tgaccttgag attatcgcca
     8401 tctttggtaa agacaggatt taccagttcc gaaaatacgt agtcgccgga aacaggagca
     8461 agcacaccgt ctttgacata ataggcaagt tctttttctg tagctgtcgg atagagttta
     8521 aagaatgttt ccaagaaagt agtagcgtct ttgaccgtat cggagctgac gctgacatct
     8581 gctccctatg ctttcggttc atactttgat ttctgtacca ccggagcaag ggtaggattc
     8641 tgggtaatga cccattgcac catctgtata cacatgaacg gtcacggtgt aatcttctgt
     8701 gactgccagt ttccaagcca tcaagtgtga ctttgtccac gagcttgatg tcttttccgg
     8761 caacaatcat tttttcgcct gttttcttgt cggtagctgt ggtatggatt gtgatttctt
     8821 tttcatattc atcagtaagt gtccctaaat ctatggtcac tttttttctg gatacgacca
     8881 cctcaaaagc tggaatcagc ttaaatccgg cgttggaatc acactttaat tcctcaatca
     8941 cataggtatc ataaagtaat gcacctttac tgttgtctgg ttcagaagta ccaaaccata
     9001 caccgtcctc actggatttt ccagcatttg tatttacctt acggaaagcc caacttgaag
     9061 cagtagagaa ctgaccgttt ttgtctgtga ctacaatatg ggattcgcca aatgttgccc
     9121 cctcaaagga ataattgacg ttgccatctg tagagggtgg gattggaaga agtctttttg
     9181 accttttcat ttcccacatt caacttcacc cagaactgtc caatgtcctg tccttcgcca
     9241 gtgtagatgt aaccgccaca ttcatagcat cccttatttg ctttcacaaa tgctttcgca
     9301 ccagcgtaaa ctttattctg taccgcctgt gagatttcat tataggaagc tctgacgtta
     9361 tcacattgcc agccgagctg ttcactcaat ctctgccaga caacacactg ttccaataag
     9421 taaccctgct tgtaattcaa gtttgtatga gaagcagtat attgcttgac gtactctaag
     9481 gaaagagcaa tgtccgcaat ctgatcgcta ctcatatggg agcttgcgtc ataccttgtt
     9541 ttatatccat ttttgaaatt tgtattgatg tccacgcaat aggcagtttc gccttcaact
     9601 ttcatgtgtc cctcatttaa tgtggatttg atagaaccat cattcataac atgttcaatg
     9661 tagccgacac gttctgctga ttctgtccag tattgattgc cagaagcatg aaccaccgtc
     9721 acaggcaatg cggtaagaat ggttgcaatg gcgagcgtcc ctgtcagcaa tcgctttact
     9781 agcttattca tattttctct ttctcctttc gattttgggt atgaaaaaaa gacgctctga
     9841 aaagaacgtc tggaaataga aaaggaacgc caaaagtgta cgttccatag tctatataag
     9901 tattcaattt ttactaatgt gccatgccat atctttgcat atctgggaaa taagacgtat
     9961 caaggctcat tcattagtag taaattcgta gtaatatcac gatttatttc ctattgaata
    10021 tgcttgtaga tacagtgttt atggggataa ccagattttt agagcgtttt tttatataaa
    10081 aatataatgt ttgtatacaa aaatattaaa gacattttag taagttttgt atataagcaa
    10141 acatgtattt tttaatatag ttatctaaat attattttat atacaataaa atatgactcc
    10201 taaaaataaa aaatatatca taaatataaa taagtagata taggttttaa tttgatttat
    10261 aaacctatta taaaataaaa ttcatgattt ttataagcaa taaaattttt aggaggatat
    10321 atgatgaata aaagaatgaa actaattcca tatgaaataa atgaaaatct aagaggtgca
    10381 aaaaataaat tcccatatgg aataaaacaa atgaatgcta ggggaatgtg ggatgaaggt
    10441 tatactggta aaaatattgt agttggtata atagatacag gttgtgatat atctcatcct
    10501 cttttaaaag gaaaaataat tggtggtgca aattttagtg atgacagtaa tggaaataaa
    10561 aatatatatg aggattttaa tggtcatgga actcatgtgg cgggtattat agctgcatct
    10621 aactataata atgaagttat gggagtagct ccagattgta aattattaat agcaaaagca
    10681 ttaaataaag atggtaccgg aacatatcaa agtataatta atgctattaa ctttgctgta
    10741 aataacaagg ttgatattat atctatgtct cttgggggaa acaaagatga taagaattta
    10801 aaaaatgctg tcatgcaagc agtaaaaaat aatatttctg tagtgtgtgc agcaggtaat
    10861 aatggagatg gtgattctag tacaagtgag tatagttatc cagccagtta tgctgaggta
    10921 atagaagtag gtgcaataaa tgaaaactat ttggttgaaa agtttagtaa ttcaaatact
    10981 acaatagatt tggtggctcc aggaagaaat attatatcga cttatatgga taataaactt
    11041 gctattatga gtggtactag tatgagtgca ccatacgtat caggctcatt agcactaatt
    11101 aaagaatggg caagagagga gtttgaaaga gatttagatg aagctgaact gtatgcacaa
    11161 ttaataaaat gtacgagagc gcttggaata cctagaacgg aacaaggaaa tggatactta
    11221 tatttaaatc tttataaata caagaataat agcaaaagat aatttttgat ttgatgataa
    11281 aatagctata ttatatagag tcgagacaaa taataaaaat tacttaggtg taagatttta
    11341 catcaatgta taaaggtatt aagtgataaa atttataaac atattagcta gttagaattg
    11401 aaaaataaat atacgatatt atagatagca catctggaaa aggtgtgttt ttctatgtac
    11461 ttatacatat aaaagattta taagagatgc aaaagtacta taagttagaa atttttctat
    11521 gaaagattag aaatacgata cagtgttttg atattataaa atgaatataa aatataaatg
    11581 ataaatatta tcaataagta ttgaatttca aaatcaattg tgatattcta tatttagaaa
    11641 aataaagaga tttcagataa agaaatcttt tgttttttag atgttagtta gctataacta
    11701 actattgatt aattaaaaaa tactacagga ggtaaaaatg agtaaaatat atattgttta
    11761 ttggagtgga acaggaaata ctgaaaaaat ggcaaatttt gtggctgaag gtgtgaagtt
    11821 aaaaggtaag acaccagaag ttttagatgt gagcttactg aaaccaagtg atttaaaaga
    11881 agaagataaa tttgcattag gttgcccatc tatgggagca gagcaactag aagaggggta
    11941 tatggagcca tttgtttcag aattagaatc tatggtatca ggtaaacaga ttggattatt
    12001 tggttcatat ggatggggca attgtgaatg gatgagagac tgggaagaac gtatgcaaaa
    12061 tgctggtgct acaattattg gtggagaagg aattacagca atggaagacc caaatgaaga
    12121 agcaaaagat gaatgtgtag aattaggtaa attattagct gaataaagat atatactata
    12181 aaagttagta tatataataa ttaatggtag tagtatataa tactaaaata gactaatgct
    12241 tatgatacat aacaaaaata caattagtga agatagataa aatattattc gcttttagta
    12301 taagttagat ttataattta atgtcgtttt ttaacaaaaa caacttcatt ataaaaaaac
    12361 aataattgat tgataacaga agtgataaag gttgtatctt gtgtaaaaca aagcatattt
    12421 gaatcagtat taacctatgt ttttattttc cattgtagga aaagtaataa atgacatctt
    12481 atatttttta catgtcttag cagtatgaga aatcatatct ttaaaacgcc gccattggag
    12541 acaatgagcg gcgttttggt gtaacggctt aggagttccc tgtgaacttt ggtagctgat
    12601 ggttcgatta tcctttatgg ggcgcttaat ggcaaaaaat aaagacaaag cctttgttat
    12661 tgctagacgc tgatacccgt aggtgtactg ccatacaccg tagagtgtta gcaagctgtt
    12721 agcaaatgcg cttagttttt tgcaattttt actttttaaa tataaatcat ttttcactag
    12781 aaatcaagat actcgtaccc tttggacgtt cagaaatgag cgtccttttt atatttcaga
    12841 aagaaaggag aatcaaaaaa ttatgataaa gattatgaaa cgattgctta cgggtgtcct
    12901 tacccttgca accgtcttta ccgcattacc tactactgcg gttcatgcag cgcagacaca
    12961 atactggacg gaatctgcgg aacgtgtagg aatcgttgaa aaggtaatga atgatggttc
    13021 tatcggctca acattcaacg agggtcatat gaccgttgag ggcgaggacg cttactgcat
    13081 agatataaac acgaatttca aaaatggcta taagaccaga gctgacgcaa gcacacgcat
    13141 gagtgccgac cagatagctg acgttgcttt atccattgaa tacgtcaagc agtacacgga
    13201 cagtcataaa gggataagca gtcagcacgc ctatctgtta agacagttag tggtctggca
    13261 gagattgagc gttcatttag gctggggttg cgataacgtg cgagctgcct atgatgaaat
    13321 tcccaaagca gtacaagatg aagtgttcgc aggtgcaaaa gcctttgtca aagaaaacaa
    13381 aggacgctat gactgttacg gttatatcta cactggcgag ggacaggaca taggacagtt
    13441 ctatgcaaag ctggctgtgg gaaatggcaa gattcagaaa tcttccagta ataaggggat
    13501 tacagaatct aacgactgct attcaatcgc tggtgcgact tatggtgtct acgctgataa
    13561 aagctgcaaa gaacagcttg ccacacttac gacagataac agtggaagca ctgaaactgt
    13621 ggagctaaaa gctgcgacat attatgtgaa agaaaccaaa gctcccaaag gattccagtt
    13681 agataagaac gtgtatactc ttacggtcaa agttggcgaa acagctatct tgaaagtgag
    13741 cgatacaccg aaagtcacag ataccttgat tgagcttttc aagattgata tggaaacttc
    13801 caaagcagac ccacagggta atgcgagttt ggagggtgca gagtttatct ggaagtatta
    13861 cgacggatat tatacaaaag ataatcttcc agcagagccg acacgcacat ggacgacaaa
    13921 aaccaaagcg gaaaaaggca gcgacaatac aatccactat atcacccgtt tagctgattc
    13981 ctataaagtg tctggcgaca gtttctatac acaaaacggt acggtctgcc tgccgctggg
    14041 aactatcacg gtagaagaaa aggctgctcc aaacggttac ttattggagg gcgcatatat
    14101 gcaggctgct ggaagttcag aacaaataaa aggcttgtat gtggcgcaga ttacagaaaa
    14161 tggcgagctt gcagcgttaa gcggcagcaa ccagtattcc gtatctgaca aggttatccg
    14221 tggcaaaatc cagaaacgtg accttgaaac aaaagatacc aaagcacagg gcggagcgac
    14281 tttaaaagat accgcctttg caatcatttc cttaaatgat aatgctgtct tggttgatgg
    14341 caagctgtat aagaaaaacg aaaccgtaaa aacaatccat acagggattg acggtatcgc
    14401 tgcgactgat acagataccc taccttttgg aaaataccgt ttagaagaaa ccagcgcacc
    14461 agagggatat ttgacagaca gtgcaaaagc ggttgaattt gaaatcacag aaaatgggaa
    14521 aattgttgat ttaacagatg aagaacattc catttacaac cagattaagc gtggcgacat
    14581 tgagggcgtg aaaatcgctg ctggcacaca caaacgtctt gcgaatgttc cgtttaggat
    14641 cacaagcaag acgactggtg aaagtcatgt tatcgtgacg gacgcaaacg gtcagttctc
    14701 aacttctgct gactgggttt cccatacaca gaataccaac gagggcaaga ccagcgagga
    14761 cggtatctgg ttcggtactt ccgcaccaga cgacagcaaa ggtgctttgc cttacgatac
    14821 ctatacgatt gaggaattgc gctgtgattc caataaggga atgacgctga ttcctgattt
    14881 tgatgtggtg gtttcaagag ataagaccgt gattgactta ggtacgctga cagatgaata
    14941 cgaaccagaa atcaatatcc atacaactgc tgctgataag gcgacaggtg aaaaatcaat
    15001 cgtcgctggt aagagcgtga ctatcgttga taccgttacc ttagacggtc tgaaaaaagg
    15061 tacaaagtac cagcttaaag gctggcagat gataaaatct gaaaatgcac agctacttgt
    15121 tgatggaaaa cctgtggaaa atgattacac atttaccgca acagacagca agatggaggt
    15181 tcagattgaa tttacattca atgccagcga acttgcaggc aaagagcttg tcacttttga
    15241 agaattatac gacgtgacga atccagacga gcctatcaag gtagcggaac acaaagatat
    15301 tgaggacgag ggacagaccg tgaccgtcaa ggaacaacca gaatcccctg tcacaccaga
    15361 ggaatccgat acgccagaaa cacctagcag agctggcagc tctccaaaaa caggtgacaa
    15421 tacaccttta accgcactgt tggctatgct tggaatttcc agtgctggtc ttatcttcgc
    15481 agcctacaag agattttata aaaagaaagc tgactaatac gggaggtatc agctgatgaa
    15541 agtaacgtcc cgttcaccgc ccattataaa aaagtcagcg tatatctgac aatctgtcta
    15601 aattctgctt gatgatggct tatctttctg aatggggatt gtcaagggtg caaagcacaa
    15661 cgcctgcctt gataatccac aggcagacag atattatccc gacagcagaa tgacactatc
    15721 aataaaatat agaaagaaaa gaggtatatt atatggaatt aaaacacgtt atcccaaaca
    15781 tggaaaaaac attcgggcat ttagaatttg caggtgagaa taaggtggaa cagcgtagga
    15841 tcaacggacg catggcggtc gtttcccgta gctttaacct ttattccgac gtgcaaagag
    15901 cagatgatat tattgtggtg ctgcctgcgt ctgctggtga gaagaacttt gaatcagagg
    15961 aaagggtaaa acttatcaat cccaaaatca ccgcagaggg ttataaaatc ggcacaagag
    16021 gtttcacaaa ttatatcctg tcagcagacg acatggtgaa agcatcagga ggtatcgaat
    16081 ctatgagatt agcaaatgga atcatcattg ataaagaaaa aacattcggc gtattgaagt
    16141 tctcggctct gcgtcgtgag gtacatatcc agaatgagga cggtacggtt tctgaagaaa
    16201 tcaaggaacg tacctacgat ttgaagtgta aggaacaagg acgcatgata caggtaagta
    16261 ttcctgccac tgttccgtta aaggaattta actacaacgc agaggtggaa atcatcaatc
    16321 ctgtggctga tactgtggca accgctactt ttcaaggggc agatgttgac tggtacatca
    16381 aggcagacga tattgttttg aagaacaaag taactacttt aaatggttct caaaacaaaa
    16441 ataagtaatt ttatttattc ccatatgatg ttacataagg tataattatt ctataaatca
    16501 aaagaaaaga ggcaattatg tatgattaac tatagtattc ttaaaataaa gctgttaagc
    16561 attattgaaa gtattgatga ttatacctct gaaccaatca tatctgtacc ttgcacaact
    16621 ttaaaaaatg atttagaaaa cgataccatg actattgatg ttttagaata ctgctgtaat
    16681 attattgagg attggtacaa ggataactta tcacatattt gtagcaatca atatgtgata
    16741 agacctgaaa aacataaaaa gaatacacaa accattaaag agattaatga agatattcaa
    16801 gttaatcggg aagaatatga aaaatatttt tccaatcctt caattagtca agctaatcca
    16861 tcaatcagcg gtgataaaat atttattgtt catggacatg ataatggctt aaaaaatgaa
    16921 gttgcacgat ttttagaaaa attaaagcta aaacctataa tacttcatga gcaagctagt
    16981 gagggtgaaa caattattga gaaaattgaa cgatattcaa atgttggttt cggtatagta
    17041 ttgtatactc cgtgtgacaa aggaggatta gctaacgatg gtgcggaatt aaggtgtaga
    17101 gcacgccaaa acgttgtctt tgaacacggg tatcttatag ggaaattaga aagaaaaaat
    17161 gtttgcgccc ttgtaaaaga tgatgttgaa aaaccaaatg acatttctgg tgtagtatat
    17221 gtttcctatg atgaaagtgg tgcttggaag attgaaatcg ctaaggaact gaaagcggct
    17281 ggttataaca tcgattttaa taaactgttt taaaataatg tgtatgcagg cagtgaatct
    17341 acaaggtttg ctgctttttt cgtggaaagg aggattttat ttgccacatt ttcaaaagcg
    17401 tggaaaccgt atcagagcca gcgataaatc gctggtcttt cgtttttcgg ctggttcgct
    17461 gctcctgctt ttccttacgg tggtcttgct gctaaaccta aagacaatcg cagcaacgga
    17521 ctggaaatcg gttcatttcc tacaagatgg cagcgtccat ctgacggtca caccctacat
    17581 gatagtttct gtcattgcgg cacttcttat ctgtggggcg gctgccctct tataccgcag
    17641 attccagtat gacagggtaa agcagctctt tcaccgtcaa aagctggcaa agatgattct
    17701 ggaaaacggg tggtatgaat cggaaaccac acaggactgt ggatttttta aggatttggc
    17761 agcgaccagc aagaaataga aaatcaccta ctttcctaag atgtattacc gtatggaaca
    17821 cggactgttg catatccgca cagaaattac attggggaaa tatcaagacc agctcctgca
    17881 tttggaaaag aaactggaaa caggcttata ctgcgagctg gtgtccaaag aactaaagga
    17941 cagttatgta gaatacgtcc tgctctatga tacgattgcc aaccgtatca ccattaacga
    18001 gatacaggcg gaacatggca gcctgcgtct gatgaaaaat gtctggtggg aatataacaa
    18061 gctcccccat atgctgattg ctggcggcac tggcggcggt aagacatatt ttatccttac
    18121 cattattgag gctttgctaa aatgcaatgc gatcatgtat gtgctagacc ctaagaacgg
    18181 tgcgaaacgt ttctacttga aaagagtaga cacagcacaa tcaactaaat ttgtgctgga
    18241 gaactgaaca cacgaatagt cgaatgatgg ggtaacgccc tgaaaggcta ctttaatact
    18301 ccgactggca gaattatgag taaacataag actgtcagaa gctcggtgaa gtcggcaaag
    18361 agataccgta acagataatg ctgacgaaaa atacccagag gtgggtgtcg tatcccatat
    18421 gccggaagtc tataaaatat ctatggttag tatgtagatg agcggtctac tgacgaatct
    18481 ttgaatgtac aggtctatat cgggactgga tagaaatgtc cagtgacaca aacagtgtcg
    18541 ctactgtggg tgagtaagac tcgtttttat gaaaacccat ataatgttac aggcattatc
    18601 aaagtagcag gcttagagaa gaaacctacg gatatatgta cagatagtgt gttaggaacg
    18661 ttgaaagctg gcaacatgga gattttacta tctatgaagt gttcacatca taaggttatt
    18721 gtgagattag caatgatata aggtgtgttt atgccagtga tagtggtggc acagtaccta
    18781 tgaaaccatg ataacaaatg gtggagggat agccactagt cattgaagaa tagaattact
    18841 aagaatggta tgggttcgag taagactaag agatgtactt actcctgtca ttaaggaggg
    18901 tacagaccat gctaaagaaa gacaagctcc gctataacga atattatgat atgcaacata
    18961 tttatgatga gttatacgca cggagcaaaa atggtaataa cttttataag ttactggaaa
    19021 ttattggttc agaacaaaat atctgcttgg cataccgaaa tttaaaaaca aacagcggaa
    19081 gcaaaaccgc tggtacagat gggctaacga ttgatgatat taaacatctg tgtgatgaag
    19141 atattattat gaaagtccga agcagtctgg ataattacca gccaaaatca gtcagacggg
    19201 tttttattcc taaatctgga agcgacaaaa tgcgacctct cggaatcccc tgtatctggg
    19261 acagacttgt acaacaatgt atcttgcaag tcctagaacc gatatgcgaa cctaagttcc
    19321 ataatcattc ctatggattc cgtgcgaatc gaagtgctca ccatgcactt ggcagagtaa
    19381 ccagtttgat aaacatatca aagtatcatt actgcgttga tgtagatatt aaaggattct
    19441 ttgataatgt caatcacggg aaattgttaa aacagatttg gacactcggt attcgggata
    19501 aaagattgat ttgtattatc agtaaaatgc taaaagctga aattgacggt gagggcgttc
    19561 cagagaaagg aactccgcaa ggcggtctgt taagtcccct gctctcgctc atagtcctta
    19621 atgaattgga ttggtgggta agcagtcaat gggaaacttt ccagccaaag caccgtaata
    19681 aaaacggttg gtttcaatat gcaaagaaac atacgaggtt aaaaagtggc tttattgtgc
    19741 gttatgctga tgattttaaa attatgtgtt cgacctatga agaagcacaa cgattctatc
    19801 acagtaccgt agattttctt aacaaaaggc tgaaattaga aattagtcca gaaaaatcaa
    19861 aggtagtaaa tctgaagaag aattcatcag atttcctagg attcaaaatt aaagtgattc
    19921 cgaaagggaa aacaaaacat ggatatgtag ccaaaacaga tatgaaccag aaagcattaa
    19981 agaaagcaaa aacaaatcta aagttaaaag tcaaagatat agtcagacat acaactactt
    20041 tccagattgc acgttataat ctggcggtta tgggaatgca gaattattat tgtgtggcta
    20101 caaatatata caacaattta acagaagtaa gttacgctct cttaccgacc accagagttc
    20161 gttttaaaaa gattgcaaag ctaatacctt ttgaaactac aagtcaagat ttccagatga
    20221 aaacaactgg aatacgacca caaacaaaaa ttattatgat tgccgatact cctctgttgc
    20281 caatcaacgg agtaaagcac aaaaatccgc tgaatttctc acaggatata tgcaacttta
    20341 cagaacacgg aagaagcaga atacataagg aaatagcatt ggtaacgaaa gatgagatac
    20401 ggattctttt agagtataaa gacaacacaa agagtgttga gtttaatgat aatcgaatct
    20461 cggtatttat agcccaacaa ggcaattgct atatcactaa tcgaaggcat agcccgactg
    20521 atatggtttg tatctacaag aatattactg aaacagatag agataaatac caaaatcttg
    20581 tatttgttga aataccaata tcaaaagcga tactgactga atcagtagaa caggcaaaga
    20641 tgtggttgat gaactatggc ttgaacagtc aacaaaagaa aaagctgaac aaaatacgag
    20701 cgaactatgg ataccaaaca ataaaataac cagaacttat gaatctattt gaatatgatg
    20761 gaacgccgta tgaagcgaaa gtttcatgta cggtgtgaag cgggggaaaa cttggagata
    20821 acatcaaaga gttacctatc gctatctgac cttgcagatt taggtacggt catgggaaat
    20881 gtgtatcaca ccaaagaaga aatgattgat tgtgtcaatg ccttttatga gggcatggta
    20941 caaagaagcg aagaaatgaa gcgacaccca gactataaaa cgggcgaaaa ctatgcctat
    21001 cttggtttac cgccctgctt tcttatcttt gatgaatatg tggcattttt tgaaatgctg
    21061 gggacaaaag aaagtgtaag tttacttagc catttaaaga aaatcgtcat gctggggcga
    21121 caagctggat atttccttat cgttgcctgc cagcgtccag acgcaaaata tttctcagac
    21181 ggtatcagag ataatttcaa cttccgtgtg ggattggggc gtatcagcga attgggttac
    21241 ggtatgctgt tcggttctga tgtgaaaaag caatttttcc agaagcgtat caaagggcgt
    21301 ggttattgcg atgtgggaac aagtgtcata agtgagtttt acacaccctt agttccaaaa
    21361 ggacatgatt ttttgcagac tatcggcagg cttgctcaag atagacaatc catgccagaa
    21421 caacatgaaa aggagtgtga aaatatatga gatagtttcg gttttgtata tagatatggg
    21481 tgtatttgga taagcaatcg aaacggcagg aacggtcagc gaagcgaaca gaccgtgtat
    21541 gccgttgctg gtgtggcgtt agccacgcca gcagatacaa gcccctcatt atctaacaga
    21601 ggggcacaat tacggcggaa acttccacaa aaacctttgc aagaccgcat gaaataaggg
    21661 tttttcgtac atttcgcctt ttgtgataaa gccttgaaat tatcacaata tcacaaaatt
    21721 atcacgattg ggggtatgac aactgaatga aactgatttt ttaaccgcct tgaaagaaaa
    21781 acgctgtgca tacggcgtat cgcagacaag gcttgcaatc atggcaaata tcagccgtga
    21841 acatttgagc cgtatcgaag caggaaaggt aacgctgacc gaggacatga aacatacgct
    21901 tttagaagcg gtggaaaaat tcaatcccaa caatcccatg ttcctgctat ttgattatgt
    21961 gcgtatccgt tttcccacaa tggatataaa gcatatcatc aaggatatat tgaagctcaa
    22021 tatcggctat atgcttcacg aagattacgg acattacaaa tacaccgagc attaccatat
    22081 cggagatgtg tttgtgtatg tatcgcagga cgaagaaaaa ggtgtcctgc tggaactgaa
    22141 aggcaaaggc tgtcgccagt ttgaaagcta tctgctggca caagagcgaa gctggtacga
    22201 cttcttcacg gacgcattgg ttgagggcgg tgtaatgaag cgtcttgatt tggcaatcaa
    22261 tgaccgaacg ggcttacttg atattccaga gctgatacga aaatgtgaga atgaggaatg
    22321 tatttctaaa ttccgttctt tcaaaagcta tggttctgga gagctggtaa agcataatga
    22381 aacggataaa tacggtatgg gacacacgct gtatattggt tcattttcaa gtgaagtgta
    22441 cttctgctgt tatgaaaaga attacgaaca gtacgccaaa cttggcatac cgattgagga
    22501 agtccccata aaaaatagat ttgagatacg tttgaaaaat gaacgtgcct attatgctgt
    22561 tgtggaactg ctgacacatt atgacgcaga actgacagca ttttctatta tcaatcaata
    22621 tatccgcttc gctgataaag aaccagacaa gcgaaaaagc gactggaaaa ccaatgtacg
    22681 ctgggcttgg ttcattggcg aggggcgacc gcccgtaaag ctgacaacac agccagaacc
    22741 ttacacgctg gagcgtacac tcaactggct acaaaggcag gttgcaccga ccttgaagat
    22801 gttgaagcag atagacaagg acaatcagac ggagtatttg gaaacgattg aaaagcaggc
    22861 aaaactgacc gaacgacact accagataat caagcaacag acagccacgg ttgaagaaat
    22921 ggttgccaac gtaaaggagt gatgaaaaaa tgtgggaatt attgaaagac ttattgctgg
    22981 tatcatttgg aatgggtatc ggtgtagtct tgatgtgcat tttaactgtc ggcaaagaag
    23041 ctgacagatg tatggaagaa atcaaagaaa gagaggacaa ttaaatgaat ttcggacaaa
    23101 atttatacac atggttttta agcaacgccc agagcttagt gcttatggcg attgtggtta
    23161 ttggtatcta cttagggttc aaacgtgagt tttctaaact tatcggcttc ttggtagttg
    23221 cccttgttgc cgttggattg gtattcaatg ctggcggtgt gaaagacgta ctgttagagc
    23281 tgttcaataa gattatcggt gcgtaaaatt ttattgtggt gctgatatga gaatgatata
    23341 atgtggatat gaagaaagcg acaaattaga atttatggag gttatttcta tgagaaattt
    23401 ggatataaat gtattacatt tggatgaact acaaatattt ggattatggg gaaagtctaa
    23461 tgataaaaca atttctaatg atataaaaat tctctcaaaa aaatatcatt ccatggtttc
    23521 cttacctgaa ggtaaagtat ttccttattt tgtacttgct agaaattatg atgaacaaag
    23581 taaggatttt gaaatgttta taggtagtac gatagataaa aatgaattag aaagcttaat
    23641 cctctctgct ggcgaatacg ctaagataac agtaaaacca aaattaggat ttttatgggg
    23701 cgtttctgta ggggaagcaa aacgatactt ttatacaaaa tggcttcctg aaagtgaatt
    23761 taaagcatta aatatggaat atgaatatca cacagagaaa agtattggaa agcatccaac
    23821 gattgatata ttttttgcca ttgaaagaaa gtcataaaat taaggttatg cctttgtatc
    23881 agcattttta tggaaaggag ggttacgtta aatgagttat gaagcacaga ttgaaatgtt
    23941 gacttgttca gataataaac aagcatataa cgcattgaaa gaattgttgt tagtttgtga
    24001 gcacagcaat gatttatata ttttttttga taaatttgtt gatatgatga ataataaaac
    24061 aaattctttt attcgtacaa gaggattgcg attaattgcc tatcatgcga agtgggataa
    24121 tgaaaataag attaattcta ttattgaaca atggctaagc catatcgaag atgaaaaacc
    24181 aattacggca agacaatgta ttcaagatgt aattgttatt gctaaatata aaccagaact
    24241 tataaatgtt attcttgatg cattaggaca ttttgaccaa atttatgatg atagtatgca
    24301 gaaattagtt tataaagata gacaaaaagc aataaaaata attcgccaat atacttggta
    24361 aatttcagtt tgacaacttc atatccatac acacaaagca gttagtctta ggattgactg
    24421 cttttttctt acccaaattt cagattggag tgatgtaatg aacgatattt ttagagatat
    24481 gcaggcaaaa atcggctgtg aaaacatttc agacctgccc tcttacaagc gtaaggtatg
    24541 gcatgaaatg aaacaactga acattgccga ctatgaagaa aaacagttag aagatttttc
    24601 aaagtatgtg tttggtatgt catgccagac tttaaaagat gtgataaaac aacagaaagg
    24661 acgtgaggaa caatgcagga aacaagggtg ctggtggaaa cgagcggaac aactggcgaa
    24721 gaaacaacgt catactggtt cgacctgccg atagaggttg cagagtttga agaaatgcta
    24781 ggtgtcggtg cagaaagtgg ggagtaccgc attattgaaa aggcattgcc ctttgctgat
    24841 gaagtccatg agcatacaag cgtgtaccag ctcaatgaat tagattatat gtatcgccag
    24901 ctttcaagcg atatgcagga agaatataca atgcttcata cggtgtatga aaatttagaa
    24961 gcactttata tttgcaggaa tgtaattacg ctttatcccg actgtaaaag catgattgat
    25021 gttgcaaggc aaaagctgat gaatgaccct acattcaaac atttatccga ggactgtcaa
    25081 gaatactatt ttgactttga aacctatgct tcacacttgc aggaacacgg gaaattttta
    25141 gtaacggaac acggtatctt tgaattgcca gagtaagaaa agaggtggtg cttatgattg
    25201 atgatatggc agtttatatc gctaatcttg gcaagtataa tgagggctat ttggtcggtg
    25261 cttggtttac tttccctatt gacgaagaag atgtgaaaga aaaaattggt ttgaatgaac
    25321 aatacgaaga atacgcaatc catgataccg acaacttccc tattgagatt ggggagtatg
    25381 tttccattga agaactcaat gaaatgtatg aaatgataga ggaacttccc aactatatcg
    25441 tggattgtct ggacgaattt atcggtcatt acgggacgct ggaagaagtc gtagaaaata
    25501 aggacgatat ttactattat ccagactgcg aaaccatgac ggacgttgcc tactattaca
    25561 tagatgaatt gcaggcactt ggcgatattc cccctagctt gcagaactat attgactatg
    25621 aagcatacgg gcgagatttg gatatgggcg gttgcttcat tgcaacaagc cgaggaatgt
    25681 gcgagatacc atattaacgc tggaagatca gcgacaagca ttgttgctac tgacggcgta
    25741 tttttcttta agggacagtc tgaaacatgg ctgtcctttt ccattttgaa aggagttgaa
    25801 agaacttgaa aaagattaga agctacacgg gtatctggaa tgtagaaaaa gtcttatatg
    25861 caatcaatga ctttaacttg ccctttcccg ttacctttac acagattaca tggtttgtga
    25921 ttacggaatt tatcatcatt ctgtttgggg atatgccccc actttccatg attgagggag
    25981 catttttaaa atacttcggt attcctgctt tcctcacttg gttcatgtcg cagaaaactt
    26041 ttgacggaaa gaagccgtac agctttttga agtcacagat aacctatgcg ttgcgaccta
    26101 aaatcactta tgcaggaaaa gccgtaaaac tgcaaaagga aatattcaac gaaacaatca
    26161 cggcagtaag gagtgtgaac tatgttcccg attaaatata ttgataataa ccttgtctgg
    26221 aacaaggaca atgaggtgtt cgcttactac gagctgatac cctacaatta cagttttcta
    26281 tccgcagagc agaaatttat cgtgcatgac agtttccgac agcttattgc acaatcccgt
    26341 gagggtaaaa ttcatgcctt gcagattgcc acggaaagct caatccgaag tatgcaggaa
    26401 cagtcaaaaa gattggtaac tggaaaatta aaggaagtcg cttaccagaa gatagacgaa
    26461 cagaccgaag cgttagtatc aatgattggg gacaatcaag tagactaccg cttttttctt
    26521 ggcttcaagc tcatggttac ggaagaacag ctcaatctga agaacctaaa aaaatcggcg
    26581 tggctgacgt tcaaagaatt tctccatgaa gtgaaccaca cgctaatgaa cgactttgtt
    26641 tccatgccga atgatgaaat caaccgttac atgaaaatgg aaaagttact ggaaaataaa
    26701 atatcaaggc gtttcaaggt tcgccgtctg gaaatcaatg actttggcta tctcacggaa
    26761 catctttacg gcagggacgg tatcgcctat gaagattatg agtatcagct accaaagaag
    26821 aaattgaaaa aggaaacgct gataaaatac tacgacctta tccgcccgac aaggtgtgtg
    26881 gttgaggaaa gccagcgata tttacgattg gaacatgagg acaaggaaag ttatgtgtcc
    26941 tattttaccg tcaatgcgat tgtaggcgag cttgattttc cgtctagtga aatcttctat
    27001 ttccagcaac agcaatttac ctttcccgtt gatacgagta tgaatgtaga aatcgtggaa
    27061 aaccgaaaag cattaacgac cgtccgcaac aagaaaaagg aactgaaaga ccttgataat
    27121 cacgcttatc aagctggaag tgaaaccagc tcaaatgtag tagacgcttt agacagcgtg
    27181 gacgagctgg aaaccgattt agaccagaca aaagaaagta tgtataagct ctcttatgtt
    27241 atccgtgtgt ctgctgatga tttggacgaa ctgaaacgcc gttgtgatga agtcaaagat
    27301 ttttatgacg atttgaatgt gaagttagta cgtcctgctg gggatatgct ggggcttcat
    27361 tctgaatttc taccagccag caagcgatat atcaatgatt atgtgcagta tgtaaaatca
    27421 gacttcttag ctgggcttgg ttttggtgcg acacagcagt taggggaaaa cacgggtatc
    27481 tatatgggct attccgttga tacgggaaga aatgtgtacc tgcaaccgtc ccttgccagt
    27541 caaggtgtaa aaggtactgt aacaaatgcc cttgcttctg cttttgtcgg ttcacttggc
    27601 ggtgggaagt cgttctgtaa taacctgctt gtgtactatt ctgttttgtt tggcggtcaa
    27661 gcggttatct tagacccaaa atcagagcgt ggaaactgga aagaaacact tccagaaatc
    27721 gcccatgaaa tcaatatcgt aaacctaaca agcgacaagg acaacgcagg gcttcttgac
    27781 ccgtttgtga ttatgaagaa tgtgaaagac gctgaaagtc tggcaatcga catactaacc
    27841 tttttaactg gtatttcctc tagggacggc gaaaaattcc ccgtacttcg taaggcggtg
    27901 cgttctgtta cccagagtga aacaaggggc ttgcttcatg tgatagacga gctacgccgt
    27961 gaagataccg caatctcacg caatatcgca gaccatatcg acagctttac ggactatgat
    28021 tttgcacatt tattattttc ggacggaacg gtggaaaatg ctatcagttt agataatcag
    28081 ctcaatatca ttcaagtagc cgaccttgta ctgccagata aggacacgac ttttgaggaa
    28141 tacacgacca ttgaattact atcggtttcc atgctgattg tgattagtac ctttgcctta
    28201 gattttatcc acagcgaccg ctctatcttc aaaatcgtag accttgatga agcgtgggcg
    28261 ttcttaaatg tggcacaagg cgaaacttta tctaacaagc tggtgcgtgc tggacgagct
    28321 atgcaggcag gtgtgtattt cgttacgcaa tcttctgggg acgtgtcaaa ggaaagtctg
    28381 aaaaacaata tcggcttaaa attcgctttc cgttctactg acatcaacga gataaaacag
    28441 actttagaat ttttcggtat cgacaaagag gacgaaaaca atcagaagcg acttcgtgat
    28501 ttggagaacg gacaatgctt gttgcaggac ttatacgggc gtgtcggcgt agtacagata
    28561 cacccagtct ttgaagaact gctacacgcc tttgatacca gaccgcccgt acagagaaat
    28621 gaggtggagt gatgaaagaa aggatacaga gtgcgttcac aaaaaagaag atgttccgct
    28681 ttctcaagat aacggtgttt gtcgtggcac tttcccttat cctgctttca ctacttggaa
    28741 cggtggctca tgcaacgggg cttgtggacg atacgataaa tacagaaaat ttatattcta
    28801 aatatccgtt atccaactat cagcttgatt tttatgtgga taatagctgg gcttggttgc
    28861 cgtggaactg gctggacggt atcggaaaat cggtacagta cgggctttac tgtattacta
    28921 acttcgtttg gactatcagt ttatatttaa gcaatgccac gggctatgtg gtgcaggaag
    28981 cctataagct ggacttcatc aatgatatgg cagacagtat cggacagagc atacagaccc
    29041 ttgcaggcgt tacctcaagc ggtttttcaa cctctggctt ctatgtcggt ttcctgcttc
    29101 tcattatctt ggtggtggga atttatgtag catatacggg actgattaaa agagaaacca
    29161 acaaggcact tcacgccgtt atcaactttg ttgtggtgtt tattttgtcg gcttcattta
    29221 ttgcctatgc tcccgactac atcaaaaaga taaatgaatt ttcaagcgac atcagtaccg
    29281 cttctttgga cttgggaaca aaaatcatgc tcccaaactc tgacagcgag ggcaaggaca
    29341 gcgtggactt gataagggac agcctattct ctattcaagt ggaacagccg tggctactct
    29401 tacagtttgg gaacagcaac gcagaggaaa tcggaacaga ccgtgtagaa gctcttgtat
    29461 cggcaagccc agaggacgag gacggaaaga ccagagagga agttgtgaaa acagaaatcg
    29521 aaaacaacga caataataat ctgacaatac cgcaagtcgt aaaccgttta ggtatggtgt
    29581 tcttcctact gttctttaac ttagggataa caatatttgt attcttgctt acgggtatga
    29641 tgttgttcag ccagatactt tttatcatct ttgccatgtt tctgcctatc agctttttac
    29701 tatccatgat accgagctat gaaagcatgg cgaagcaggc aatcgtgagg gtatttaata
    29761 ccataatgac aagggcagga attacgctca ttgtaacggt ggcgttcagt atttccagta
    29821 tgttttataa catttccacg gactatccgt tttttatggt ggcgttcttg cagatagtat
    29881 gtttcgctgg tatctatatg aaactgggcg acttaatgag tatgttctct ctgaatgctg
    29941 gcgacagtca aagcatggga cgaagaattt tccgcagacc gtatctgttt atgcgacata
    30001 gagctaggcg tatggaacac cgtattgcaa gggcggtaag tgctggcggt atttcgggcg
    30061 gtgtggctgg tgcggtagct ggaagtgccg ttgctaacaa acgagcggaa agaaaaaata
    30121 cagcttctaa agaaaatcgg ggcaatacca cttccagcat gggacagcgt gcaggctcaa
    30181 aggtaggtgc tgtcttagat acgaaaaata aagtgaaaga caaggcaaat gctgtcaaag
    30241 aaaacatcaa ggatatgccg acacagaccg cctatgcggt gtattccgca aaggaaaagg
    30301 caaagtccag cgtgtccgac tttaagcgtg gcatggtgca ggaacagcag tccagacaga
    30361 cgggacgatt ggaaaagcag gaacagcata ggaaaaatat tgcggacaag cgtatggagc
    30421 ttcaaaaggc acaagaagca aggcaggcac agcgaaaggc tgacggatca gcgacaacgg
    30481 gagctacccg tccccatgag cgaccagcca cagctttaac tgtttcaaag acgggtgcgg
    30541 aaaaaatgca ggaaatcaaa cgccctgcca cagcgaccac ttcaaaagca agtgagccag
    30601 tcaagacaaa tgttatcaaa gagcgtccgt tatcttctgg tacttctgat aggaaagcga
    30661 cccagtctac acagccagta cataggcaga atgtagaaaa agtggtatcg caggaaacac
    30721 gtcaaaatta caaagcagtg cgtactacaa agacacagac ctttgaacaa tcaaagagaa
    30781 caacggaaca taccgaaaag aaccgtaacc ttgtaacaaa gaaaggacaa aagaaaaaat
    30841 gaaactgaaa catatcgctc tcattagcag tctgtttcct atcctctttt ctctggtgct
    30901 tttctttggt gtcctcatta gtgcggacag cgacgacgag aacagcaatt tttcttctgg
    30961 cattacgggt atgaacctat ccgcagaagt cttgaaacat cagcctatgg tggaaaaata
    31021 cgccagagaa aacggtatct ccgagtatgt caatgtgcta cttgctatca ttcaagtaga
    31081 aagtggcggt acggcagaag atgttatgca gagttcggaa agtcttggct taccgcctaa
    31141 ttccttagat acggaaagct cagtcaagca ggggtgtaag tattttgcgt ccctgctttc
    31201 ttcctgcaaa aatcaaggta tcgacgattt gaatgtggcg atacagtcct ataactatgg
    31261 cggtggctat gtgggctatg tggcaggaaa aggaaagaaa catacataca atcttgcaga
    31321 gagcttcgcc cgtgaaaaat cgggcggtaa aaaagtaacc tacaccaacc cgatagccgt
    31381 tgcgaagaac gggggctggc gttatcagta tggcaatcaa ttttacgtcg aattagtcaa
    31441 tcagtattta actgttccgc aggtgtcggg agaactggca caaaaggtaa tgaatgaagc
    31501 cttaaaatat caaggctgga agtatgtgta tggtggttct tctcctacga cttcctttga
    31561 ctgctcggga ctgacacaat ggtgctatgg aaaagctggt atctccttgc cgagaaccgc
    31621 acaaatgcag tatgacgcta cccaacattt gccactctcg caggcaaagg ctggcgattt
    31681 gatattcttc cattccacct ataacgctgg ttcgtatgta actcacgtcg gtatctatgt
    31741 aggaaataac cagatgtacc atgctggcga ccgtgcgagg cgttcctaac tgaaaaggtt
    31801 aggcatttat gaaagcacgt taaacaattt cataaagaga actgataatc tgaaaagtcg
    31861 aatgacggag taacgccctg aaaggcttta gctaagactg cgacgtgcga ttaagcagat
    31921 aagaacttga ttgtgcgaag ctcgcagaag tcggctgaga gtacacccta aagacgttaa
    31981 agcggtacag gaaatgcgaa ccagaggtgg tcgagtgtat gataggtcgg gcttccataa
    32041 aatactcatg gtaagtatgt gtttcagaaa gtgaaatact gacaaaattc tgaatgtacg
    32101 tgtctaaacc gcgggattga ctaacagcaa tcgcttctat tgaagagctt gtggggatta
    32161 gtaaaactgg tcactatgaa agtccctata ctgtcaaggg cagtatcaag caagcaggca
    32221 cagaggaatt acctaaaagt atatatgtaa ggataagatt atcggaacgt cgtaagctga
    32281 aaacatgtat acccttatcg gtaaatgaag tgttcacaag gaaaatttct aaagaaatat
    32341 aacttgggtt aatttcagtg aaagtggtgg cacagtacct atgaagcgat gataacaaat
    32401 cgtggaggga tagccactag tcaatggtag ttaaaaatca attcattaaa aacaattagg
    32461 ttcgagtacg actaaaatag gcagtcactc caaaggaggt acaacgccga ctaatgacaa
    32521 ccaaaacaca gaagaaaaag gctttaaggt tcaatgaata ctacgataca caagacattc
    32581 aagacaaatt gtatcaactg ggaaaagaaa actataagtt tactaacctt gttgaactaa
    32641 taacagataa acgcaatgta atgcttgcat atcgaacaat caaaacaaac aaaggaagca
    32701 aaacggctgg cacgaatagt aataccatta ctgaattagc tgaaaccagc ccagagttac
    32761 tgacagagta tgtaaaaata aggcttaaaa attatattcc ccatgcggtt aaacgagtag
    32821 aaattccaaa accaaatgga aaaacacgac cgctcggaat accaaccatt gaggatagac
    32881 taatacagca atgtatcaga caaatattag agccgatatt agaagctaaa ttctatgaac
    32941 actcatacgg atttcgccca gataaaagta catctcatgc tattgctcgc ttctatcatc
    33001 ttgcacatag tggatttcac tatgttgtgg atatagatat aaaaggattt ttcgataatg
    33061 ttaatcatgg aaaactgtta aagcaattat ggacgttagg cattagagat aaaaacttga
    33121 tttccataat atcaagaatg ttaaaagcac ccgtcaaaaa caatggagtt cccaacaaag
    33181 gtactccaca aggtggaata ttatccccgc tactggcaaa tgtagtatta aatgaacttg
    33241 actggtggat agcaagccaa tgggacaacc accctacaaa ctatccatac aaacatcaaa
    33301 gaagcaagtt acttgcgtta aaggaaacac agctaaagga aattaaaatt gttcgttacg
    33361 ctgatgattt caaaatcatg tgtaaagatt gtgatacagc acagaaaatc ttcatggcta
    33421 ccaaaaagtg gctaaatgaa cgattacatt tagaaatcag tccagaaaaa tcaaaaatca
    33481 caaatattcg taagaattat tcagaatttc taggattaaa gctaaaggtt aaaaaaggta
    33541 aagctggaaa atacacaaac agaagccaca tgagggacaa ggctaagaaa cttgcagtag
    33601 aaaaacttaa agagcaaatc aaaacaattc agaaaaaccc aactagagaa gctgtaaata
    33661 aatacaattc tacggtgtta ggattacaga actattacag tatgtcaact atggtaaatt
    33721 atgatttcgt agagattgcc tatatagtca acaagagcct gctttgtcga accaaacaca
    33781 ttcgcagtaa gaatggaact ataacgcaaa catatcaaaa gttttataag gaatatggat
    33841 ataaaaagat attcatagca ggtactgcct tgtttcctat tgcaggaatt aaattcagag
    33901 agccaaaaat gatttcaaaa gagtgtacca gatatacggt tgagggaaga aatgcaatac
    33961 ataaaagatt acaaatgaac atgagaatta ttcattatct catggaacag acatcacccc
    34021 tacaaggaac aactgaattt aatgataatc gaatttctgt atatgttgca caaagaggaa
    34081 aatgcaagat tacaggaaaa gaattaaaaa tcggaaatat ggacttgcac catataaaac
    34141 ctaaattcaa aggtggttca gacgagtata aaaacttgat atatgtaaca aatgaaatac
    34201 atattcttat acattcctcg gctaaggaaa ccacggaaaa gtatttgaaa atagtaaaac
    34261 cagacaaagc acaactaaat aaaatcaatc agtatagagt tatggttgga aataatgagt
    34321 tagattaaaa cgactaccat tgagggaacg ccgtgtgata ggaaactatc atgcacggtg
    34381 tgaagtgggg gaaaacttgg agataaaatc aaaaagttac ctatcactat cgataggata
    34441 tgcagacctc aatagtagct actggcaaca gcacttaatc ggtgcaggac gagtaaaaca
    34501 atagaaagga cttagaaaat atgtttaaga agaataagaa acagacagaa aatatcaaag
    34561 aaaaaaaggt gcgtacagtc aagataggca cacataagaa aaccgtgatt gcattatggg
    34621 tggtgcttat cgcaagcgtg agttttggga tatataagaa ttttaccgcc attgatatgc
    34681 acaccgtaca tgaaacagaa accatacaat taagacttac cgatactaac gggattgaaa
    34741 atttcgtgaa gaactttgcg aagtcctatt acacatggaa caacagcaaa gaagcgattg
    34801 aagcaaggac gcaggcaatc agcggttatc tgacaaagga attgcaggac ttgaatgttg
    34861 ataccattag aaccgatata ccgaccagtt ccacggttac agatgtgatt gtttggaata
    34921 tcgaacagtc aggaacggac actttttctg ctacctacga agtagatcag cagataaaag
    34981 agggagaaca gacaacagca gtcaaggcaa cctatacggt aaaagtctat gtggacgttg
    35041 acggggatat ggtaatcata cagaacccta cgcttgcacc aacaatcgaa aaatcggact
    35101 atgagccaaa gacaccagaa gcaggcgcaa gtgtggacgc tgataccata aatgacgcta
    35161 cttcattttt ggaaacattc tttaagctgt acccaacagc gacggaaaaa gagcttgctt
    35221 attatgtatc tgataatgta cttgaaccta tcggcaggga ctatctttat tctgaattga
    35281 taaacccagt ctttacaaaa gacgggggca atgtgaaagt caaagttgcc gtgaaattcc
    35341 ttgataatca gacaaaggca actttagttt cgcagtatga gcttgtacta tataaggata
    35401 gtaactggaa gattattatg taagacattt tgttggcagg ctttattttc ggatatagtc
    35461 tgccttttct gtagcaaatg tactgcactg acaagctatt tcattgtatt ttaagcaatg
    35521 aaatctttct tgacaatttt acccgaaatc atatatttat agctattgac tttctgcttg
    35581 caataagcta taatatgact ataaaatcaa attagtcata taggaggtat ggtgtatgta
    35641 taaagaagtt ggcatttctt caaataatgt tccgataaca ctttctattt ggtatacaga
    35701 aagaagtagt cctaccattg tttttttgcc gggtactatg tcacaccctc tcatgtatga
    35761 gaattttcta tgtggattga gcgaaagagg gtttaatata ataggtgttc actatctttc
    35821 acacggaaaa agccctaaaa tcaaaaaaaa ttatacaatg gaagatatgt tgcataatgt
    35881 atatgacgca acaacttacg gaatagaaaa tttcggtgaa aacatagcta tcatgggttc
    35941 cagtcaaggc gggattttag ctgcaatggc ggcaggtagg gacacaaggt taaaagccgt
    36001 ttttccacat aatatcatgc tgactacatt aaaagaaact ttgagcttaa caaaatatcc
    36061 cgcatggtcg caccgtttta tggggctgat acagtggggc tttcgtgtag gtggaaaatt
    36121 gctgcctaac tacaaggttc caggagacgc ttatttagac tatgacaaag tattttatag
    36181 tgaacaggca aaacaagatt gcttaaatga ccctatgctt ttaccctatt atcctttaag
    36241 atttgtcgcc agcttattta atgctgactt atcgtgcctg gagaatggag ctatacaatg
    36301 tcctgttatt ttaattactg ccaaaggcga tccgcttttt agctttgatt ataccaacaa
    36361 agtatttgat ttaattcatg taccgcaaaa ggaattactt actttagagc ttaatcgtca
    36421 catgattttt aatgaggaca cggacactac aataaacgca ttggaagcta ccttgcataa
    36481 atatcttgga tagcatattg attatataat cagtaaatgt tatactttta atgacgatat
    36541 aaatatttta gtcataagga ggttatgagt ttgccacgat ttacggaaca agaaaaagaa
    36601 atcataaaca gtaaactttt gatagaggga gaaaagctgt ttgcattaca cggcttaaaa
    36661 aaagtaacag ttgatgattt agtagctgct gtaaacatat caaaaggttc tttctatgct
    36721 ttttatccca gtaaagaaca tctatatgtt gaaatcaatt ttcgcttaca aaaagagtta
    36781 tttacaagta tagaaacaac cattaaaaag aaaaagtata agaaccatag agatttagca
    36841 aaggacgtta tcatgctaag tttaacgggg gtgattactt cgcctattct ttcacagatt
    36901 gatttatctt taatggacta tctgcaaaga aaaatatcgt cagatatttt tgaaaatcat
    36961 atgtacagtg atattcgtat attggagata ttggaagatt taggggttgt atttttggtt
    37021 ccgcatacgg ttattataaa atccctgtat tctgttttat cctgtttgga acaattcaag
    37081 gaggacgaag aactgaatat gatacaaaat cttttagtga atggtattat tcagcaagta
    37141 gtagcagagt aacaattttg tttggttatt tgacaggcag gtagccaaac aaatttttta
    37201 cagtttatat gactatatat ctataaacgt cataaagtta aaggaggaat gaataatgat
    37261 tgatgtaaaa aaactgtatt ttagctatac tgacaaaccg tttgtggaga atgtaagttt
    37321 ccatgttggg agaggagaaa tttttggttt tttaggaccc tctggtgctg gaaaatcaac
    37381 tattcaaaaa gttttaactg ggcttaatac aagatataaa ggcagcgtaa aagttgcagg
    37441 tacagaaata agggagcata caaatcagtt ttatgaaaat attggggtag actttgaatt
    37501 ttcaacctgc tacgagaaat tcacagcacg acaaaatctc gcctattttg cttctctata
    37561 tgaaaagcaa cctcggtcta ttgatgaatt attgcatatg gtaggcttgg aaaatgacgg
    37621 ggacaaaaaa gttgctgact tttccaaagg tatgcgttct cgcttgaatt tcattaaagc
    37681 gttagtccat gaccctgata tattattcct tgatgaacct acaagcggtc ttgaccctac
    37741 aaatagtcgt ttaatgaaag atattattct tgcggaaaag aaacgcggaa aaacgattat
    37801 cattactact cacaatatgt ttgacgcaac agagctatgt gaccaagtag cctttatcgt
    37861 tgccggaaaa gtcagtgcat tggatagccc gcataatttg attatgtcaa gaggagctgc
    37921 caaaatacaa tatacctact atgataaagg cgagaaaacc ggagaatgtc tgcttgatag
    37981 aaccacagaa gataagttgt taaaaagttt aatttccgaa aaccgtttgc tttctattca
    38041 tagtagtgag cctacattaa atgatatttt tgttgatatt acagggagga cacttcaatg
    38101 agattgggac gtttaatttg tggtgatata catttccaat ggaaatatgg tttctatttt
    38161 atctacttta tattaacagt tttatatgtg tgcgggattg ccgctttgcc ggaacattgg
    38221 aaaacggata ttgcttctat tatgatctac tctgacccgg cagcaatggg attgtttttc
    38281 atgggagcga ttgttctttt ggaaaaaagt caaaaggtat taaatgctat ggtggtatcc
    38341 cctgtaaaaa tatcggaata tatactttca aaaacagttg cgttgattgc tatttctacg
    38401 gtaatcgcat tgatattagg ggtagtttcc ggcagcaatc atttacttgg tatcacagtt
    38461 ggtacggctt tgacctcagc aatctttact atgcttggaa ttattgcagc tacaaaaatt
    38521 tcaaacttga accaatttct cattgtgatt atgccgatag aaattgtttg ctttgtacca
    38581 cctattgttg gtctgtttgt gaagttacca tatctgttcc gcttttttcc atacacagct
    38641 tgcatgaacc ttataactgg aaaaagcgtt ttactatcgt ttgatatggt acttgtaatt
    38701 gctacgttga ttatcctcta catagttgcg cgacatacag ttaagcatat gtggaaaagt
    38761 ttaggaggtg taaagctatg aaaaggatat tatccagtac catacaggta tttagacaaa
    38821 tcaagagcga ccctatgatg tttgcagctt gttttacgcc ttttgtcatg ggagctttga
    38881 ttaaattcgg tattcccttt cttgaaagaa taacagattt ttctttgcaa gcatactatc
    38941 cgatttttga tttactactt tctatcatgg ctcctgtatt actctgcttt gcatttgcaa
    39001 tgattacatt agaggaaatc gacgataaag tgtcacggta cttttcgatt acccctcttg
    39061 gtaagtcggg gtatctcttt acaaggttgg gagtaccctc aattatttcg gcggtcatcg
    39121 cttttattgt actgttactt ttttcattag aaaagttgtc cattggaatg acaatatgtt
    39181 tggcgcttct cggctcagta caggcaatta ttgtttcact tatgattatc acattatcaa
    39241 gcaataaatt agagggtatg gcagttacaa aactagctgc acttacattg ctcggaatac
    39301 cagccccctt ttttattgat agttactacc agttcgcggt tggcttcctt ccatcatttt
    39361 gggtagcgaa agccatgcag aatgaggcgg ttctttattt ctctatagga ttggtggtag
    39421 ctttagtctg gtactacttc cttacaaaac gtctgtttcg gaagctggaa ggataaggag
    39481 gcatattatg gaaaagggta gacttctgca aaacggaaaa gtaaatagag ctattcaggt
    39541 ttccaaaatc acactattta tcaacctaag tttatcgtta cttaaatttg ctgccggata
    39601 tataggaaag tcaagtgcca tgctgtcgga cgctgtacac tcggcttctg atgtattaag
    39661 cacaatcgtt gtgatggttg gaattaaaat atcagaaaaa cagcctgata gagaacatcc
    39721 ttatgggcat gaacgtatgg aatgtgttgc ttccattatt ctctctgttg cattggccat
    39781 aacaggtgct ggaattggat attcaggtat caagaaaatc ttttcagaac agtacaatac
    39841 gctttctgct cctagtggaa tcgcacttac ggcggcagtc ttatccattg ttataaaaga
    39901 gtggatgtac tggtttacaa gaaatgctgc aaagcataca aattctgacg cacttatggc
    39961 tgacgcatgg caccaccgct ctgatgcact ctcgtcggtt ggttccttaa ttggcatttt
    40021 aggagcgcgt ttaggctatg ctattctcga ccctattgca agcgttgtga tttgcggttg
    40081 tatcttaaaa gctgctttgg acattttcaa agaaagcatt aacaaaatgg tagaccatag
    40141 ttgtgacaat gctactgaaa caaagattag ggaagttgtg cttcagcagc aaggggttga
    40201 tggtattgat gaattaaaaa cccgcatgtt tggagcgaaa atgtatgtag atattgaaat
    40261 attagctgat ggtaatttag ctctttatga cgctcataga attgctgagg gagtccatca
    40321 aacgattgaa aacaactttc cacaatgtaa acattgtatg gtacatgtta atacgaatga
    40381 acttctctat tctgcatagg acagggaaga tgatttaaga ctattgaaag ggaaaggagg
    40441 gaagtaggtt atggaacaat actggtggat tatcattgtt gtggttttga ttgtcgttgg
    40501 tttacttaaa attgtaatta tgaagaagta taaccaaagg agaaattcta acaatagtcc
    40561 aaaagatagc gaggacgaca agatgtatta aggtttattg aaatctgtta tgttgtagaa
    40621 tgatgttttt agatactaga aagtgcttta agggggctaa cgttatggaa agctttgagt
    40681 acaatttttt ccaatcaaca gtattttgca tagagaaatg tatgtaggaa atatggtgca
    40741 aggcataaag caccagcgat cgagagatca acctatgaac caaaggcaca ctacatttta
    40801 tactacttga caagtaaacg agcgtttact ataatggttt acagaagtaa acgttcgttt
    40861 actaaagaag gaggttgtaa atgtctgaca caaaagaaaa tatattaaaa attgcactac
    40921 acttatttgc agtgaatggt tatgaggctg tttctgttag tatgattgcg gaagccttgg
    40981 gaatgacaaa aggagcatta tataaacatt ataaaaataa gcaagatatt tttgactgta
    41041 tcgtagaacg tatgtatcaa attgataata agcgagctaa ggaatatcaa gtaccagaaa
    41101 gcacaatagt ggtaacccct aatgcataca gaaacacaac tattgaaagt ttaacaacat
    41161 ttacttttgc acaatacgat ttttgggtga ctgatgaatt tgcgtctaat tttcgcaaaa
    41221 tgttaatact agagcaatat agaaatttaa aaatgaatgt gctttatcaa aaatgtcttg
    41281 taaatggtcc tatcagttat atagaagata tatttaatga aatgattaaa aaaggtgttc
    41341 ttcaagagtg tgatacaaaa cagcttgcta ttgaatatta tgcgccatta tttctattga
    41401 taagtgtagc agacgcagtt gaaaattgtt gggatttaaa atctcaatta accacacatg
    41461 ttaatcaatt tgtcaagaaa tatattacaa atgaaaaagt aaaataatcc tttattctgt
    41521 cctgaatgca aacataactt tttaatttca gcagaggaac tcaaaatgaa tattgttata
    41581 agtcacaaat attaactatt aaaatattac aataatggag atgagaaaat ggaatatatt
    41641 cgcagtaaaa aatattttac acccgactta cagacaaaaa ttatgggacc gaatcctata
    41701 aaattacaag aggaactatt acaaaatcat aaaatcccat ctggcagcta tgtctgtgac
    41761 cttggaagtg gacaaggttt aacttctgta tttcttgcaa aagaatatgg atttgttgtt
    41821 tttgcagttg acttatggag tgagccaaaa gaaaatgaag aatttttcaa aaaaatggga
    41881 ttatcttctg cacaaattaa atctgtaaaa gctgacgcca atgaacttaa atttgagaaa
    41941 gagttttttg atgctgtagt aagtacagat tcatataatt attttggacg tgaccctata
    42001 tatttagatg aaaaattgct tccatttgtt aagaaaggtg ggtttgttta tatcacagtt
    42061 cctggcatga agaaagattg ccatgagaaa cttccagaag agttattgtt atcatggaca
    42121 ccagaagaac ttgattatat acatgatata acatattggt ctaacataat tagtcattgt
    42181 aaaaaggcag atttaatttc aatatatgaa atggaaagta attcggaagt ttggtcggat
    42241 tggataaagc aaaataatga atatgcaata aatgaccgta aggcaataga agctggcggt
    42301 ggagattatc tgaattttat tgctataatt ctacaaaaaa aataaattct ttagaaccaa
    42361 acactataat ttgcgttcta atatgtttac cgattgacat caataatttt atctttgagg
    42421 gtaatgattt gaaaatagaa gaataattcc agtttgtaaa agtaataaat ccaaagataa
    42481 atttgtattt gaaaggagtt ttgaagaatg aaaaaatatg agtatgtaga aattaactat
    42541 aatgcgaaag gagttgtatt cctttgtact gataaacaca aagaaattat tgacagttac
    42601 gcaaaaacgg ggtatcacta cgcagggttt gtacccacag aaattgacgc aaaaggctgt
    42661 atgcgaaaga tagatttaat ttttgaaaaa gaagattaac ttattttagg gagttggttc
    42721 taatgatagg acttaaaaga cgaaatatga attatattat gaaagttggt gctaaagctc
    42781 aaggtgtttc tgtatctgaa tttagggcta tgctacaaga taccatagac aaaactatgg
    42841 aaagtacaga cccagacgtg caggcaaatt ttaaacgata ctttggaaat aagcgcccaa
    42901 caccagagga atatatttat acgattacca aaaagacaaa ggttaaggtt taaatgttgt
    42961 aaagggggct tgccgtatga aacgctttat gtacaattca gtatattcaa gcccaattta
    43021 acagcatact atttacaatt aaatacgcta taccagtagc tcgtacaaaa tttatatttt
    43081 gtgcgggctt ttttaatacc cgaaaagttt ttttaattat tttcattttt ttggaagcaa
    43141 atcacacctt gctgtacaga tatggtgcgg aaagagggat aaacactttt caattcatag
    43201 ctggaaagga ggtgagatta tgaaaccgtc tgacttccaa aaaacaattc agtgtcagtt
    43261 tgaatgtaag ctgaaaaaag ttgtcaaagg tattatctgg aactaccgta aagaattaaa
    43321 acgaagaaaa aacaaggaaa tatcttttag cgaattacca gacattacgg tagaaagtct
    43381 ggcaacatgg gacgactacg aaacaaacta cacaattttt aatgtgtgtg gcatagatat
    43441 taaagtcctt gatgatgaac ttgcagaagc tctcaaacag ctacccgaaa aaaaacgtaa
    43501 caatgtattg atgtattact tcttggaaat gagtgataca gagattgcag atattatgaa
    43561 ttataccaga ggggctgtgt tcaaacacag acaccgtgcc ctagaaacta tgaaagagat
    43621 actaaaggag aagaaataga aatgaagaaa aagtacaagc acccgtctta ttatttgatt
    43681 tcatcagcta tggacgggga tgaaactgcg attgagaaat tactggtatt ctatgacccg
    43741 tacatatcaa agtgttgttt acgtccttta tatgatgaat acggcaatgt ttatattgtt
    43801 gttgatatgg aattaaaggg acgtattcga gaagccttaa tcaaaatgat tttagacttt
    43861 gagattcata tagatataga aaccgaagaa gaataagcag ataccagcag ggcgtgattg
    43921 gttaaatccc cctctttcct gccctgctcc tgcttttata catggaagcc tacggctttc
    43981 gccgattgtt ctttgacaac tgaataaagc agacagatac gtttgtgata cagcgagcta
    44041 cggggactgt acgccatgac ctttccgaca agaaagtgag cgacccacgc agtgatccga
    44101 gagcaacttg tggaatggtc gctttgccat gacctgctat cacagaataa tgatacgtcc
    44161 gcatggtgcg gttctgccca caagaatggg aatagttgag atactaacgg agctttccga
    44221 agccgtctgt ctgcttatga taagacatac agaaaggagt gttgcaacat gaacaatact
    44281 gatgtgccta tctgggaaaa atataccctt agtatagaag aagcgtcccg atattttcgt
    44341 atcggggaaa agaagttgcg taggcttgcc gaggaaaatc tggactctgg ctgggttatc
    44401 gtcaatggaa accgtattca gataaaacga aaacagtttg aaaaaattat tgatacactg
    44461 gacgaaatct gatgtcttag agccgtagat atggtataat attagtagcg tatcacggct
    44521 cattccatga cggaaaggag ctttacacga tgtcaaaagt gaaaagacaa gacagtaaag
    44581 gacgcaattt gcgtcttgga gagagccaga gaaaagacgg aagatacgtt tataaatata
    44641 ccgatatatt cgggaagcca cagtttgtat atgcttggaa acttgtaccc acagacaaaa
    44701 cacctgctgg aaaacgtgat gatatatcgt tgagggaaaa agaaaagcag ataaaaaaag
    44761 accttgacga cggtatcgac acagtgggtg gtaaaatgac agtctgccag ctttacgaaa
    44821 agaagaacaa tcaaagaaag aacatcaaga gaaacacaaa aataggacga cagtatctta
    44881 tgaacgcact ggaaaatgac ccattgggta tgagggcgat tgatacggtc aagcagtccg
    44941 acgctaaaga atgggctatc agaatgaatg acaaaggtta ttcctacaaa acgatagata
    45001 actataagcg ttccttaaag gcttcattct atatggcgat acaagacgac tgtatcagaa
    45061 aaaatccttt caatttcaaa ttgagtgatg ttctggaaga tgatacggaa gcaaaggtta
    45121 tcctaacacc agagcaggaa gaaaaactcc ttgcctttat ggaaacagat aatgtctaca
    45181 gcaagtatca tgatgaagtt attcttctgc tggaaacggg acttcgtatc tctgaattat
    45241 gcggactgac aacacacatt gatatgctta accgtgtgat taacatagac caccagctat
    45301 tgcgtgatac agaggtgggt tactatattt ccacacctaa gacaaaaaac gggaagcgtg
    45361 agcttccatt gacagaaaga gcatatcagg cacttgaacg aattttaaag aaccgaggaa
    45421 aagcacaacc gcttgttgtt gacggttaca gcaacttctt gttcttgaac cgtgagggat
    45481 tgccaaaggt ggcaggaaat tatgaaagta tggtgcgtgg gctgattaag aagtacaaca
    45541 agacgcataa ggacaagttg ccaaacatca cgccccattc attcagacat acttactgta
    45601 cgaacatggc aaataaggga atgaacccta acaccctgca atacatcatg gggcacgcaa
    45661 acataaccat gacattaggg tattatgctc acggtacttt ccagtctgca aaggctgaat
    45721 tagaaaggct ggcttcttaa tatcgaaaat gtatcttact acttatttac tacttttggt
    45781 tgcattttca tgctggtaaa tgccatctta tgcgaggtat cttccaaaag gaaaatgcca
    45841 ataaagcccg aaaatatggg atatatcggc atttaccaac ttatgaaaag ataatcagaa
    45901 atttagtaag tttttttata aaaaaattaa gcgaaacaga tgagtttttt agagtatttg
    45961 gaagtcttga tgataagact tattcagaag gggctataag taaaaaaaat aaggaattaa
    46021 tgggactagc tatttcaatt ttaagccgat gtgatgagtg tatttgttat catattgagg
    46081 gttgtattaa ttctggagca agtaaagatg aaattataga agctataaaa ataggtgtaa
    46141 ttggtggcgg gtctattaca tatccaaacg caagatttgc aataaaaatt ttaaaagaat
    46201 ttaatatata attttgatag acgtagttta tttagactaa taataatagt ctatttaagt
    46261 ctttgaaatt aagctttatt ttctaagttc acttct
//
LOCUS       DAEMHW010000008        45757 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEMHW010000008 DAEMHW010000000
VERSION     DAEMHW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.008"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            196..1413
                     /locus_tag="KPK67_000351"
     CDS             196..1413
                     /locus_tag="KPK67_000351"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBG2457361.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKVNGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1508..1888
                     /locus_tag="KPK67_000352"
     CDS             1508..1888
                     /locus_tag="KPK67_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG2457362.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2172..2654
                     /locus_tag="KPK67_000353"
     CDS             2172..2654
                     /locus_tag="KPK67_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2457363.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2675..3490
                     /locus_tag="KPK67_000354"
     CDS             2675..3490
                     /locus_tag="KPK67_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2457364.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3542..5092
                     /locus_tag="KPK67_000355"
     CDS             3542..5092
                     /locus_tag="KPK67_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2457365.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5189..6541
                     /locus_tag="KPK67_000356"
     CDS             5189..6541
                     /locus_tag="KPK67_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG2457366.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWKSVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRYYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6672..7430
                     /locus_tag="KPK67_000357"
     CDS             6672..7430
                     /locus_tag="KPK67_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2457367.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIYNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7477..8196
                     /locus_tag="KPK67_000358"
     CDS             7477..8196
                     /locus_tag="KPK67_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG2457368.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GDTSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVYL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8320..9720)
                     /locus_tag="KPK67_000359"
     CDS             complement(8320..9720)
                     /locus_tag="KPK67_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBG2457369.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9724..10632)
                     /locus_tag="KPK67_000360"
     CDS             complement(9724..10632)
                     /locus_tag="KPK67_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2457370.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKEML"
     gene            10893..11540
                     /locus_tag="KPK67_000361"
     CDS             10893..11540
                     /locus_tag="KPK67_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2457371.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKSGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11865..14504
                     /locus_tag="KPK67_000362"
     CDS             11865..14504
                     /locus_tag="KPK67_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG2457372.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14562..15665)
                     /locus_tag="KPK67_000363"
     CDS             complement(14562..15665)
                     /locus_tag="KPK67_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBG2457373.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16026..16847
                     /locus_tag="KPK67_000364"
     CDS             16026..16847
                     /locus_tag="KPK67_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2457374.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16926..17137
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17224..18540
                     /gene="argH"
                     /locus_tag="KPK67_000365"
     CDS             17224..18540
                     /gene="argH"
                     /locus_tag="KPK67_000365"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBG2457375.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18789..19127
                     /locus_tag="KPK67_000366"
     CDS             18789..19127
                     /locus_tag="KPK67_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBG2457376.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19254..20543)
                     /locus_tag="KPK67_000367"
     CDS             complement(19254..20543)
                     /locus_tag="KPK67_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG2457377.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNNYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20623..21327
                     /locus_tag="KPK67_000368"
     CDS             20623..21327
                     /locus_tag="KPK67_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HBG2457378.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21419..22465
                     /gene="selD"
                     /locus_tag="KPK67_000369"
     CDS             21419..22465
                     /gene="selD"
                     /locus_tag="KPK67_000369"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:21467..21469,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBG2457379.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22483..23895
                     /locus_tag="KPK67_000370"
     CDS             22483..23895
                     /locus_tag="KPK67_000370"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBG2457380.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTNTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            24009..25892
                     /gene="selB"
                     /locus_tag="KPK67_000371"
     CDS             24009..25892
                     /gene="selB"
                     /locus_tag="KPK67_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBG2457381.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            25997..28735
                     /locus_tag="KPK67_000372"
     CDS             25997..28735
                     /locus_tag="KPK67_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBG2457382.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(28849..29793)
                     /gene="manA"
                     /locus_tag="KPK67_000373"
     CDS             complement(28849..29793)
                     /gene="manA"
                     /locus_tag="KPK67_000373"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBG2457383.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYILKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            29966..31327
                     /locus_tag="KPK67_000374"
     CDS             29966..31327
                     /locus_tag="KPK67_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2457384.1"
                     /translation="MELFKAESKKYEKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            31415..33304
                     /locus_tag="KPK67_000375"
     CDS             31415..33304
                     /locus_tag="KPK67_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2457385.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAIKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIYCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFSLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSVKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            33319..33774
                     /locus_tag="KPK67_000376"
     CDS             33319..33774
                     /locus_tag="KPK67_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2457386.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEGESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            33833..34150
                     /locus_tag="KPK67_000377"
     CDS             33833..34150
                     /locus_tag="KPK67_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG2457387.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGANMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            34175..35437
                     /locus_tag="KPK67_000378"
     CDS             34175..35437
                     /locus_tag="KPK67_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG2457388.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            35542..36606
                     /locus_tag="KPK67_000379"
     CDS             35542..36606
                     /locus_tag="KPK67_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2457389.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEDCIRVLRPGMTTGEIDKVARDIIGSYG
                     YAGNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            36660..37838
                     /locus_tag="KPK67_000380"
     CDS             36660..37838
                     /locus_tag="KPK67_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG2457390.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(38029..39087)
                     /locus_tag="KPK67_000381"
     CDS             complement(38029..39087)
                     /locus_tag="KPK67_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBG2457391.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKARVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKNGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            39154..39525
                     /locus_tag="KPK67_000382"
     CDS             39154..39525
                     /locus_tag="KPK67_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBG2457392.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKDAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            39562..40236
                     /locus_tag="KPK67_000383"
     CDS             39562..40236
                     /locus_tag="KPK67_000383"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBG2457393.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            40485..41324
                     /locus_tag="KPK67_000384"
     CDS             40485..41324
                     /locus_tag="KPK67_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG2457394.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            41568..41664
                     /locus_tag="KPK67_000385"
     tRNA            41568..41664
                     /locus_tag="KPK67_000385"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41602..41604,aa:Sec,seq:tca)
     gene            41815..42522
                     /locus_tag="KPK67_000386"
     CDS             41815..42522
                     /locus_tag="KPK67_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBG2457395.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(42664..43500)
                     /locus_tag="KPK67_000387"
     CDS             complement(42664..43500)
                     /locus_tag="KPK67_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2457396.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            43688..44593
                     /locus_tag="KPK67_000388"
     CDS             43688..44593
                     /locus_tag="KPK67_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBG2457397.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            44602..>45757
                     /locus_tag="KPK67_000389"
     CDS             44602..>45757
                     /locus_tag="KPK67_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2457398.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKYVSEETLETLNCIFSK"
BASE COUNT        17940 a         4977 c         7899 g        14941 t
ORIGIN      
        1 ttttaaatta tcatttaatg taatttaaat gaattgaaga tatatgttta ttgtatgcaa
       61 ttgttgtatt tttagggttt ttgtgattaa ttaaaaaatt ataaaaatcg tttacatgtt
      121 gatttttaaa aaaaaaagat atagaattat atagaaagta ataaacaaaa attggattta
      181 cgggagtgga atgaaatgat gaatgttacc ttaacagatg tgaaagaggc tagagagacc
      241 attaagaaca tagtaaaaag aactgactta cttgaaagtg ttaaacttag cgaaaaaaca
      301 ggagcaaatg tgttctataa atgtgagaat cttcaaaaaa caggttcttt taaattaaga
      361 ggagcttgta acaaaatagc gagtttaaca gatgaagaaa aggtaaatgg agttatagct
      421 tcaagtgcag ggaatcatgc tcaaggagtt gcattgggag caaaaatgac tggtataaaa
      481 tctacaatag taatgccagc aactgcgcct ttagcaaaag ttagtgcaac taaaggttat
      541 ggagcagaag tagttttaaa tggtgcggtt tatgatgatg catatgcaaa agctgttgaa
      601 atacaaaaag aaacaggagc aactttctta catccattca atgataagta tgttatagca
      661 ggacaaggaa ctatatctct tgaaattttc gaacaattag acaataaagt tgatactata
      721 ctatgcccag ttggtggagg aggaatcatc tctggtgttg ctgttgcagc aaaagcatta
      781 aatcctaatg taaaaatagt tggtgttcaa actgcaaata taccttcaat gaaagagtca
      841 ataaaaaatg gaaaagtaac aacagcattt aatgatacta caatagctga tggtatagct
      901 gttaaaactc caggagattt aacttttgaa ataataaatg aattggttga tgaaatagta
      961 gtggttgaag agacagaaat tgcagaatca atattattta tgatggaaag tcaaaaaata
     1021 gtttcagaag gtgctggtgc tgtatgtaca gctgctatat taagtggtaa gtatgtacca
     1081 gcaaaagatg aaaatgtagt ttgtataata tctggtggta atatagacat aaatacgtta
     1141 tatagaataa taggtgttgc tttagctaaa gaaggaagaa gatattcatt tagtacaatt
     1201 atggaagata agccaggaaa ctttgctgag ttaactagaa taataagtga gaatggtgga
     1261 aacattttaa gtgctaatca aggtaaatta tcagcagggg aagctctagg aaaacaaagt
     1321 gcggaattta tattagagac aatagattat gaccatatag ctagaataaa gaaagctata
     1381 gaagaaaaag gttttaaaat tatagaatta taataaaaag tgtatataat atacaagaaa
     1441 aatagtataa tagtgtatga agatacattt tatgtgtatt agattaacga attaggagga
     1501 ttttaaaatg aaacatgaag taatacatac taatgatgca ccagcagctt taggaccata
     1561 ttctcaagct ataaaagctg gaaacttatt atttgtatct ggacaagttc ctcttgtacc
     1621 agagactatg gaagtagttg aaggagatgt acaagctcaa actgctcaat ctttaaaaaa
     1681 tcttaaagct atattagctg aaagtggagc tgatttttca aatgtagtta agactacagt
     1741 atttataaaa gatatgaatg aatttggggc tataaacgaa gtttatgctg aatattttgg
     1801 agaaaataaa ccagcaagag cttgtgtaga agttgctaga ttaccaaaag acgttaaagt
     1861 tgaaatagaa gtaatagctg tattataaaa atacaatatt tttaatttga aaaggctgaa
     1921 aaactatatt ctagtgattc agccttttta taaaaaaata aatcacaata aagaacatga
     1981 taaatattgt cgaattaaaa aataaaatta aaataaatat tgcaatattt ttgagaaggt
     2041 tttataatga gtatataatt tgaaacgtgt aactattaaa ttaggcatga gttcttggat
     2101 atttttatcc tgaggattca tgcctttttt gtattgaaat tcaaaaatct atttaataag
     2161 gaggaagtac tgtgttaaat tttttcaaga aaaataaaag ctataagtta catgcagtag
     2221 tgagtggtaa cagcattaat attgagaaag taaacgattc tgtgttttca aaaaagttaa
     2281 tgggagatgg agttgcaatt attcctaata gtaatgttgt tgtagcccct tgtaatggta
     2341 aagtaactgt gcttacagaa tcaaagcatg cctttggtat ggtctcagat gagggtgttg
     2401 aaatattagt ccacatagga attgatacag ttagtttaca gggtgagggg tttaaaaatg
     2461 aagtgtctca aggagatact gttaaaaaag gtagtccaat aataagtttt gaaagagaaa
     2521 aaataaattc tcagggaatt gattgtacaa caataataat tgtattaaat catagcgaat
     2581 tttcagaaat aaattgcatg gtagaaaatg aagttgttgc agggcaagat actgttattg
     2641 aaattatgaa ataataggaa gggattaatt caatatgaga gtagtaaaaa tctttaataa
     2701 taatgcatta tctacagtaa ctaatgatag gaaagaagct attttactag gtcttggtat
     2761 aggttttaat aaacgtccag gagataaagt taatgaagat aaaatagaaa agatttatta
     2821 tgttcaagac cacatgcaaa caaaattttt agaattatta aaaaatgtta gtccagaagt
     2881 gatggatgct tcacaacaaa ttataagtct tataccagga aatgagggaa aatttaataa
     2941 taaagggata ttatctttag tggaacatat tagttttgca atagatagaa tgaaaaataa
     3001 tgttttttta cctaatttaa tgcttttgga tataaagatg atgtattcaa aagagtttga
     3061 attaggagta aaagctttag aaattatata tagagtatgt catatccatt tgcctgaaga
     3121 tgaagcaggt tacatagctt tacattttgt caatttacaa tcaaatgata atttggcata
     3181 tgatacttta aaatttgtaa aaggtagtat tgatttgatt aaagaatgtt atggattaga
     3241 attagacgaa tcgagtttaa gtacattacg ttttagaact catcttaaat ttcttgctca
     3301 aagaattttt aagaatgaaa tttgtcagga tgataaaatg attgaaatgt atgattattt
     3361 aattaacaat catcctaaaa ataaagaata tttagaaaag ttaaatgtat atattgaaaa
     3421 ggagtttagg tataaattag ataaacctga aaaaatttat ctactactac acctaacaaa
     3481 aatcttataa gaatgagatg aattagtaat agaaatatat ataattttag gggggataag
     3541 attgaaagaa aaactacttg gaggactgga aacatttgca aaggcaatgg ttcagccatt
     3601 aatgtattta tcgagtgcag gaatattaat ggtggtaggt gtactactta caaatgatat
     3661 gttattagga gtacttccat tttttaaatg ggagccaatt caaatatttg gaagcctaat
     3721 ttataactgt ataatggcta ttattaataa tcttgctgtt atatttgcta taggtattcc
     3781 tgctgcacta gcaaaaaaag agaagcacaa agcagcgtta ataggattta tgagttatat
     3841 aatatacctt acagcttcca acactatgtt aacatcacta ggtcaactag caaaagaaag
     3901 caagatgctt ggactgatag ggactggaca agctactatt ttaggtattc aagttgtaga
     3961 cacaagtgta tttggtggaa ttattttagg gtgtatagct ggatatgtat ttaatcgtac
     4021 aagcaacaaa acatttaaag gagctttaca gatatactcg ggagcaaatt ttagttttat
     4081 atgcatgttc tttgtatcca ttttaattgg agttgtgagt aactttatat ggccattagc
     4141 tcaacaagga atagcttctc ttgcaaatgt catcaaagat acaggtacat ttggtttatt
     4201 cttatatggt tttttagaga gaatattagt accaactgga cttcatcatt taatctatac
     4261 accatttcag ttttctgatt taggaggagt gcttaaagtt ggtgcagata cagtttcagg
     4321 agcctatgct atagttatga cagaaatgaa tttaccagtt gagcgtttct cagatagtat
     4381 atactacatg gcaactggat ttacaaagat gtttggttat ataggtatag gagctgcctt
     4441 tatttatact gcatataagc aaaataagaa gaaggttaag gcaacgatta ttccactaat
     4501 aattacggca gtattagcct ctgtaacaga accattagac tttatgtttg tattctgtgc
     4561 accactttta ttctttgtac atgcagttat agctggtctt tttatagctt tattaaaaat
     4621 atttgatgtt actgcattat gtggagggaa cttattagta tcaattataa tgaatactac
     4681 agtaggcgta gaaaaaacac actggccagt aatgatgata ttaggacttg cacaaattat
     4741 tgtatatttt gtagtattta gttttttaat taagaaattt aattataaaa ctccaggtcg
     4801 tgaagatgag agtacagcta caggcgaaga acaatcaaaa caacttaatt tagatgcagg
     4861 tatagaaaat ataattgatg ggttaggcgg aaaagaaaat attaatacag tagaaaattg
     4921 catcactaga ttacgtgtta atgtaaaaga tgaaagtaag attaatgaag atataattaa
     4981 tttaacgcca aatagcggga ttgtaaggaa aggaaaagat atacaaataa tttttggact
     5041 tcatgtgcat gaggtaagac gtgcagtaga agatttttta gaagcttatt aaagatatat
     5101 atttatatag caagggtaaa taacagcttt aagtgctgtt tactatttat taaagataca
     5161 tataatttat agaggaggaa taattattat gattattact attgttggtg gaggaagcac
     5221 atttactcca ggtattgtta agtcaattgc attaagagaa aaagagttag agcttgagga
     5281 aattcgttta tttgacattg ataaagaacg tcaagataaa gtagctgtag ttgtcaactg
     5341 gatactaaaa gaagatgtta aaacagatgt taaactaact gtaactaatt gtgaaaaaga
     5401 agcgtataca gatgctacat ttatatttgc acaaatgcgt gttggtaaat atgaaatgcg
     5461 tgagcaggat gaaaaaattc cattgaaata tggttgtgta ggtcaagaaa cttgtggttg
     5521 tggagggatg gcatacggac tgcgtacaat acaaccaatg ataaaggtta ttgatgatgt
     5581 agagaaatat gctaaaaaag attattggat attaaattac tctaatcctg ctgcaatcgt
     5641 ttctgaagct tgtcgtaagt taagaccaaa tgcaaggatt ataaatatct gtgatatgcc
     5701 aatagctatt atagatatga tagcggggtc attaaatatc aatgacgttc ataatatacg
     5761 ctatgattat tttggtctaa atcattttgg ctggtttact tcaatagatt ataaacatag
     5821 agacttaatg gaagagatta aggaatacat aaaagaaaat aaaatattat taccaaaatc
     5881 ttatatagaa aacttaaata aaagtcgtag tacaggaaat cgccatgctg taagtagctg
     5941 gatacatgtt tggaaatctg tttatacaat gttagaaagc ttcccaaaat atctgccaaa
     6001 cacttattta acatactatc ttttaagtaa agaaacagtg gaacattcaa acataaatcg
     6061 tactcgtgca aatgaggtta tggaaagccg tgaaaaagac ttatttgaag gtgtgagata
     6121 ttacttagaa actggagaaa tttctgaaaa agcattttat gcaggttcac atggagattg
     6181 gattagtgat ttggcagttt caattaaaaa tgatacacgt agtcgttttt tagttataac
     6241 tgaaaatcgt ggagctatac caaacatgcc atatgatgca atggtagaaa ttccagcata
     6301 tattggtaaa gatggtccag aagttattgc tcgaggtcca atacctttat tccaacaagg
     6361 attaatgatg caacaactta acagtgaaaa attgatagta gaagcttata tagaaaatag
     6421 ttatgaaaaa gctttacaag cattcacatt aaataaaact attccaagta tgaatgttgc
     6481 taaagaaatt ttggatgaaa tgatagaagt taataaggaa tactggccag aattaagata
     6541 gtaggataaa agaaagggtt tttatttaat aattagatat gttttcagag tcttttatca
     6601 aaatgtattc acaaacatat ggcaatatat taaaataaag agtgatattt ttagaagaat
     6661 ggggtgatgc aatgaacatt ataaataaaa ttttttggca agaagatagg attggcatga
     6721 ttacaaataa tttagaggca gatacacata gtcactgtat gttacaactg ttcttaggga
     6781 tagaagacag cattgaaata acagttaatg aaaaattagt taaatgtaat tgtatcatag
     6841 tagataaaaa tatctctcat tcattttcag caagaaaaaa agtttattac tctgctatta
     6901 ttgaacctac atctatatat gcagaacagc ttacctcaaa aatgaatgat tttggctact
     6961 ggatatgtga caatgatgga ttagagaaat tacgtcaaca aggaactttt ttgatatata
     7021 actctagtaa agaacaatat ttaagattta tggaaatgct taataactat ttgaatatac
     7081 ctataactct caaacattat gatgacagaa ttactgaatt gctcaattta ttacgtactt
     7141 gtaattgtga taatcatact atttcaagtt ttgcagataa agtgtcccta tcagcaagta
     7201 gattatcaca tctgtttaaa gaacaaatag ggataccttt aaaaagctat atcttatttc
     7261 atcaaatgga gtgtgctttt agagaattac tttctggaaa aaatgttacc gaagcttcta
     7321 tgactgctgg attcgatact ccatcacact ttgcagggac agttaagcga atgatgggaa
     7381 tgcctgtttc tttatctttg aaagatagcg agtttttgaa agtatattaa atattctcgt
     7441 tatataatga agttgtacta agaaaggggc tttgttatga ataaagaata tttagaagaa
     7501 acaaaaatgt taaattataa tgaaccacaa cttaagcttt tggtatcatc aaagaattgg
     7561 ttggaattag atgattttca taaaataaaa tctatttatg agtttgtaca gaatgatatt
     7621 ttatttggat ataatgcttc tgatatgtta agcgcaacac aggtattaaa tgatggtatg
     7681 ggacaatgca atacaaaagc tactctttta atggcattat tacgtgcagt gaatatccct
     7741 tgtaggcttc atgcttttga tgtaacaaaa gattttcaac gaggtgatac atccaaattg
     7801 atttcactat tagcaccaaa atatattctt catacttggg tagaggtatt ttatcaagat
     7861 aggtggattg ctctggaggg tgtcataact gataaaaaat atttagaagc aattcagaag
     7921 aaatttttta atcatggtgg aacgtttaaa aagtatgcaa tagctacaaa tgatttgaaa
     7981 aatacatcta ttgattggga tggtaaagat acttttatcc aaaaagaagc tatcgtgtat
     8041 gattatggta tttttccatc accagatgta tttttttcta ctcattcaca acatatgtca
     8101 aaattaaaaa atttcatata tgtatatctt atcagaaaaa taatgacaaa aaatgtatgt
     8161 aaggctagaa acaattatat tgataaaaac gaatgagtgt ttaagaggta actatatagg
     8221 aaatatatag aataattaaa actgtactgg ttaaaatgaa taaaaaggag ttatcacaga
     8281 gcaaacttaa attgttcata ggataactcc ttttctgtat taacttctaa taatttgatg
     8341 tctttttata aaacatatag atacaattaa accagtaaat gcaagagtag ctgctattga
     8401 aaatgcaaca tctattccat gaatgttaga cagcatgttt gaactcatat gagctttctt
     8461 agtagcactt gtcattatag taataagtat agcagaacct atagctccag atatctgacg
     8521 gagtgtgtta ttaattgctg tggcatgaga tataagttct ctatctaatg ttttaattcc
     8581 ccatgtagtt aatggcatta aaaccattga aataccaatc atacgaatac aatacatcaa
     8641 tgacacatga attaaagatg tatctctgcc taaaaaagcg aaggataaag ttcctaaaaa
     8701 caaacatcct gttcctaata ttgatagagc atgaggtcca tatttatcta aatgacgtcc
     8761 tgcaattgga ttgagtacaa ccatcaatat agcaccaggt aacattaatg aacctgaaga
     8821 taatgcagaa aatcctctta cagattgaac gtacagagga atcattaatg tagcagacat
     8881 cattgaggca tatacaatca taattaatat tgtagaaata agaaatggtt tatttttaaa
     8941 aaccctcaat tctaaaaaag gttctttaga ttttaattga cgcaatgtaa ataaaattaa
     9001 tgacataatt cctattaata ttggtaaata tgtggcaata ttagtccacc cataattacc
     9061 ttgattagta aatccaatta ataaaccacc aaaacctaga gatgatagta ctattgaagg
     9121 tatatctaat tttagttttt gagtttcccc aacatttctt aatagaataa atgatagtat
     9181 aacatctaat atagcaatag gggaaagaaa cagaaataaa ctatgccaac caaatgaatc
     9241 tactaaccat cctgataaag ttggacctat tgctggagca aatcctactg taataccaac
     9301 taaactcata gctgcaccac gtttttcctc agggcataaa tagagtataa taacttgtaa
     9361 aagtggcatt agagaaccag aacccattgc ctgcaacaca cgagcaatca acatagttga
     9421 aaaattatta gaaaaaagtg caataataca tcctatagaa aagatagaca ttgatgtaat
     9481 aaataacttt ctagtactaa atcgatttat taaataagct gttgtgggta caataatacc
     9541 tgcaataagt agataaattg ttgttaacca ctgaccaaca tcagcagtaa ttttaaaatc
     9601 tgacattata ttaggtaatg ctgttgacaa tagtgtttga ctgagagaac ttataaaagc
     9661 accagtcatt acaattgcta ttattaaatt tcctttttta ttgttacttt gggtttccat
     9721 aatttataac atctcctttt ttaattgatt tttaatgctg tttaataaaa gtacttttgg
     9781 tcttaaagtg tcttcttttt ctaaatatgc aatcattgca tccataatta aagaatcata
     9841 ttgttgattt tttccctcga cgtataattt ttttattgct tctagtaatt taggattttc
     9901 aaggagagaa gtatcagttt tgattagttc ttctatatct tgtatcgttt taaaaaatag
     9961 cgatttatta tgaagggaaa agtaaacttc atcttcttgt ttatgtaaaa acattgattt
    10021 ttcaattaaa ggatttagat tatccatatt ttctacaact aaatctagaa tttgtatagt
    10081 aaatttggca ataaagtata catttatttc tcttttttgt acatgaataa tgtatataaa
    10141 ctgatgaatt agagcatcca ataagaaaac taaatcaaat ataatttttt ctattttatc
    10201 accatagtta tcaaataagc ttaatttgta ttcattcaat aagttagctc gtgattttgt
    10261 ttgcaattta cttgtagcct tacgttgctc ttcagaaaaa ttttctctta tataggtatt
    10321 tatggtagtt ctagaaaaaa tatttttcca tacagcaatt atctttctgt ttaacttttc
    10381 tgatgatgga atgtcattat tttcgtttat ggaatgtata atttctaaaa acttttcatc
    10441 ataataattg attatttcgc agataaaact ttccttagtt tcaaaatgct tgtaaaaagt
    10501 agctttagac attttacact cttttacaac atccgatatt gaagtatttt gaaaaccctt
    10561 ttggtcaaaa attttttgtg caatagttat aatgagcctt tgtttttctg tcactttatt
    10621 atctcctttc acgcaaaaac gtttagtact atatagtacc tacaagtttt aataaatata
    10681 ttatacatca tttggaaaaa aaattaatat atagactgta atatgaaatt tttgaaaaat
    10741 ttgttgttat ttttagtcgt ataaaaaaga aacttaagag tttcttaaga attgtttaaa
    10801 gaagttgcaa taaattagtg gtatgattat agtatgaaag agaatgtctt gaaatttttc
    10861 tgtatcaaat tccaattatg gaggtgtagt atatgaaaaa gaaaatttgt gtagcaataa
    10921 tttttgctct atttatagtt tttgtagaaa tgtttattga tagctctagc gaaggaagta
    10981 aaattatgac agctgaatta agtaaataca ggaaaggcgt aaatgtatta tcaataaaca
    11041 atattatagt agcgaataaa aaatatagtt tgcccaaaga ttattctcct caggaaagca
    11101 gtgaagcgag agatgctttt tataaaatga ataaagatgc tcaaaagtca ggattaaatt
    11161 taaaagcttt tagtacatat agaagttatg aatatcaaga tagattattt aaatcatatg
    11221 taaaagaaca tggagaaaaa gaagcaaata gattttcagc taaatctgga gagagtgagc
    11281 atcaaactgg acttgctttt gatattggag gagatgacca gtcttgttgg gcaaataaaa
    11341 agttcaacaa tacaaaagaa gctaaatggt tgtatgaaaa tgcatataaa tatggtttta
    11401 tacttagata tccagaaggt aaagagaata taacaggata tatgtatgaa tcttggcact
    11461 atagatatgt tggaactgaa catagcaaaa actttgcaat gaataatttg actttagaag
    11521 aatatttaca tataaattaa gagatggttt gttaaaatta ggttaaaata aaaaataacc
    11581 tagactatta gctgtgtaaa gtgatataat gtaaatacaa tttaagataa ctactcgaag
    11641 gggagtagct tacacgaaag tggtaattaa tcgtcaagac agtagaaata ctcggttaat
    11701 tagcaaggga ttccttgata gcaagacctt caaaataagc attaggtatg tttgttttga
    11761 ggtctttttt tattcaagga atttttattt aaaaaaattt aaatttcaag tatacataag
    11821 agcacccaaa cataaaataa atttattgaa gaggagggca acaaatgtct tacaaaagta
    11881 cttatcaaga agtaattaaa aatttagact caagtttatc tggtttgagt aaagaaagag
    11941 cagaagagtt gttagaaaaa aatggagcta atgaattaaa agaagcagat aaggttccta
    12001 cttataagct tttcctagaa agttttaaag acccattagt aataattcta ttgatagctg
    12061 ctttagtaca aatattttta ggtgaaacag tggagtctat aataattttt gcagttatta
    12121 ttataaactc tgttctgggt gttgtacaaa caaaaaaagc agaaagttca ttagaaagtt
    12181 tgaaaaattt atctgcacca aacgcaaagg taataagaga caataaaaaa atgacaattc
    12241 cagcaaaaaa tctagtagtt ggagatattg tattcttaga agctggcgat tacataccag
    12301 cagatggaag gcttatagaa gcacaatctc tcaaggttgt agaagggatg ttaactggag
    12361 aatcagaacc agttttaaag catacagaaa aaataagtga agatgtaagt ttaggtgacc
    12421 agaaaaatat ggtatttagt ggtgctgtag ttgtttatgg tagaggttct tttgtagtga
    12481 ctggaacagg tatggatact gagatgggta aaatagctgg gcttttagaa agtgctgaaa
    12541 ataaacttac accattgcaa caaaaaatag aggattttag taaaaaactt ggtgtaggta
    12601 ttgtaatact tgctctttta atatttataa ttcaagtagc aagaaattac tttatggctg
    12661 gaaatggaga aatgacacaa attattttag attcattcat gttctctgta gcagtagcag
    12721 tagcagctat accagaagct ctatcatcaa tagtcactat agtattggct gttggaacaa
    12781 atagtatggc aaaaaaacaa gccataataa gaaaattacc agcagtagaa actttaggtt
    12841 caacaagtgt aatatgtact gataaaacag gtactcttac acaaaataaa atgacagtag
    12901 ttgattatta tatgtatgga actaataatg atgagttaga taaagaaaaa gtaaactatt
    12961 cttatcatgc taaattattg actatggttt ctacattgtg taatgattct catataacta
    13021 gtgatggtaa agaagttggt gacccaacag aagtggcttt aataaattat tcaagtaaaa
    13081 atgatttaga ttatgataaa ttaagaagta agtatatcag aataaatgaa ataccatttg
    13141 actcagacag aaagcttatg tctactgtaa atagtatata tggagattat ataatgttta
    13201 caaaaggtgc tccagatatt gtgtttagta gatgtaaata tgctttgaaa aatggttcaa
    13261 aagtagatat aacagatgag ataatagaag aatataaaca taaaaatgaa gagttttcaa
    13321 atagagcact tagagtatta gcttttgcaa taaaagatgt cccagaagaa gattttatac
    13381 cttcaattga tgacgaacat gacatgacat tggttggaat aatggcaatg atagacccac
    13441 caagagaaga agtaatggat gcagtaaaag aggcaaagag tgctggtata aaaactgtta
    13501 tgataactgg tgaccataaa acaacagcag cagcaattgc aaaagagatt ggtataatgg
    13561 aagaaggaga cttggctctt actggaaaag agcttgatgc tttgagtgaa gaacaactat
    13621 atgaaaaact agaaaatata agtgtatatg ccagagtctc gccagaaaat aaaattcgta
    13681 tagtaaaagc atggcaacat aagaataata taacagcaat gactggtgat ggtgttaatg
    13741 atgcacctgc attaaaacaa gcagatatag gaatcggtat gggaagtggt actgatgtag
    13801 caaaagatgc ttctgctatg gtattggttg atgataactt tgcaagtata gtaaatgctg
    13861 ttgaagttgg tagaacagta tacagtaata taataaaatc aattacttat ctatttgcag
    13921 gaaacttagg agctatagta tccatagtat ttgcagtgtt tgcaggttgg tcaaatccat
    13981 ttacagcttt acaacttttg tttataaact tagtaaatga ctctttacca gcaattgcat
    14041 tagggtttga aaagtcagaa aaaaatataa tgaataaacc tcctagacac ccagatgaaa
    14101 gtatactagc aggaggaaca ttaaaggcag ttgctataag aggttgtata ataggtgtag
    14161 ttacaataat tgcacaatat ataggattac aagtatctcc agaacttgga gtagcaatgg
    14221 cattttcaac attgatatta gctagaataa tgcaaacatt acctgcacgt tctaataatc
    14281 aaacagtaat aaaattaggt tttttcagca ataaatacgt tataggagca gttttagtct
    14341 gtcttgggct ttactcttta acactaatac cagtgctaag aggaatattc tctataccaa
    14401 caacgtttgg attgtaccag ttggggattt gcctagggtt agcattaatt tcatcaatat
    14461 taatggaact taacaaatta ataaatagaa gtgaagtaaa ataatcaaaa aattaaaggg
    14521 catattgatt ttaacaatat gccttttata aatagtctaa tttattctat attatcgatt
    14581 atttggaatg cttttataac ttcaggtaat tctttttctt caggcattct aaatcttatg
    14641 aaatttttat caagtccagt aaaatcactt ccagatataa ctcttatttt taatttagca
    14701 aactcttgtt gtaaatctat gtccttgttt ttatgttcaa ctgtcataat tgatacagta
    14761 tcactagttt cagctatatt aaggtttttc catggaatta ataattgatt ttttatatct
    14821 tttgtttttt cttttaattc ttctataaat tgaacatcat ctaataaatt tgctgcaata
    14881 ctacgagata tttcagacac ttcgtaagga gtagaaatct ttttaatagg gctaacaagt
    14941 tgttctggaa gaactgcata tcctgctctt agtcctgcta aaccgaatcc ttttgaaaat
    15001 gtttttaaag caattacatt atcataatta tttagaagct tgacagctga attttctttt
    15061 ggcatgtatt caccataagc ttcatctacc ataactgcta tatcatattt agctgcttcc
    15121 tttactatat tttcaattga tgataaaggt ataatttgtc ctgtaggatt gtttggattg
    15181 tctatgtaaa ttactttata ttctggattt attttttcaa taaattcttt ttcattaaac
    15241 ttgaaattat cctctttctt taaaagaaca taatcataag ttgcaccatg catttttata
    15301 tctgtctcat attctgagaa ctgaggtgaa tatcctaaaa ctttatctcc cttttcaata
    15361 aatagtctat ttaaaaggta aataacatgt atagaaccat ctgccaaaca aattctgtca
    15421 gtatctaatg ctataaaatc tttccaataa tcgactatat tatcttttaa agcaattgaa
    15481 tgaggatatc ctcttatcat atcaaatttt aaagcattaa aagcttctac agctttgttt
    15541 gagtaagaca cggtatttat accttcacca cagtctatct cgaatacatc ttctgaatgt
    15601 ccagattcta ttgcataact ttttttcaac tgcatttcaa tagctttgtt aatttttagt
    15661 ttcatacttc atcactcctt atttagtcaa taataaatgt tataaaagtt gctagattta
    15721 aaatattatg aaaatatata atagtaaaaa atgaatataa atattataat agctcacttt
    15781 atttaatatg attatatatc atatattgta aaatttctag cattataaga ctataaattg
    15841 aaaatattca gaaaaatact acaagaaacg atttattttg ctcttaaaat aagttaaatg
    15901 aatgttatta attttgtaaa ttgaaatagt ttaagtataa agttatgata taatctaata
    15961 agggtaattc tttagattat attaaggaaa tataggtata caataaaaag ttatggagtt
    16021 ggattatggg atataaatta attgtaacag atatggatgg aactttatta ggcaataatc
    16081 ataaagttac agatgaaaat aaaactgcac tacaaaaagt tataaaaagc ggtattaatg
    16141 taactttagc aacaggaaga gccttcgatt cagctaaatg caatgtagat tttcttaaag
    16201 aggacatgcc aataatagct tgtaatggtt cattaataag agagcaaaat ggaaatataa
    16261 tatattcaaa taagattgat acaagaacat gtttaaacat tttagatgta ttagataaat
    16321 acgatattta ctaccaatgt aatagtatag attctatgct ttcgaaaaaa atagaaggac
    16381 gtgaagatag attaagtgta tttttaggct ctgaaacaga agtaattgta aaagatgatt
    16441 tgagagaaga aatctttaaa aaagatatac taaaatttgt aatcatagaa gaaaaaaatc
    16501 catctatttt ggatgaaata agaaaagaat tgagaaaagt tcaaggaata aagataactt
    16561 cttcatggcc aaacaatatt gaagtcatga atgaaggtgt tgataaaggt aatgcagtta
    16621 aaattttagc tgaaaaaatg aatatagaca gagaagatat aattgcattt ggagacaatt
    16681 acaatgacat agaaatgata aagtttgctg gtcttggggt agctatggga aatgcagaag
    16741 aattgattaa acaagaagct gattatgtta cagatacaaa tcaagacagt ggagttgcaa
    16801 aggcaattta taaattccta gagttaaaag aatcacttag ctcttaaaac agtatataat
    16861 aggaaaaaat aggttgacta ttttgtagat agtaattata atacaaatag ataaaacata
    16921 aaaatttaga ttcagtgaaa aagagagtag atatagagaa aatctcagcg aattggggga
    16981 tggtgtgagc ctaatgataa cactttatat tgaaaagaac tttggagaat actacttgaa
    17041 attcagtatg gtagtaggta atctcgcctt aagagaatag agtgggtaaa cattgtttac
    17101 caactagagt ggtaacacgg aatatttcgt ctcttggtct ttttgaccaa gagatttttt
    17161 agtttcattg agttacaaga cttataatta aaatacaaaa aattaaaata tgaggtgaaa
    17221 gttatgaaat tatggggcgg acgctttaga aaagcggaaa atcaacttat ggaagaattt
    17281 aataaatctt ttggctatga ttgtgtactg tacaaaaaag atatcgaagg aagtgtagca
    17341 catgttcata tgcaagtaaa atgtggactt ttaacagaag aagaaggaaa gtctataaca
    17401 gaaggtttaa aagggatact tgaagatgta gaaaatggaa agttagtatt ggatggagag
    17461 tatgaagata tacatagttt tactgagata aatttaattc aaagaattgg agatgttggc
    17521 aagaagcttc atacagcacg tagtagaaat gaccaagttg cagttgatat gagattgtat
    17581 gctaaagaaa aagcaaatga tttggtgggt ttaatatctg aatttaaagc tactataaaa
    17641 gatgttgctg acaaaaaccc agtgatgatg ccgggctata ctcatcttca aagagctcaa
    17701 gtagtgacat ttaagcatca cttaatggca tattatagta tgtttgatag agatgagaaa
    17761 agaataaaaa atgctataga aatattagat gaatcacctt taggctgtgg agctttagca
    17821 ggaactacgc atgatataga tagaagtata acttgtgaac aattaggatt taaaaaagta
    17881 gtagataatt ttatggatgg agtaagtgat agagattatt tactagagct aatgagtgat
    17941 ttttcaatta taatgatgca cttaagtaga ttaagtgaag agttgatact ttggagttct
    18001 caagaatttg gatttgttga gatagatgat ttatatacaa ctggaagtag tataatgcct
    18061 cagaagaaaa atccagatgg tgcagagctt ataagaggta agactggcag agtatatgga
    18121 aacttatttg gtctttttac agtaatgaaa ggaataccat tagcatataa taaagacatg
    18181 caagaagata aagaaggttt tttcgatagt gtacatactc ttgaaatgtg tatacaaata
    18241 atggatagaa tgatagccac actaaaggta aatgaggata aaatgaaaca agcagtaaaa
    18301 aatggttttt taaatgcaac agaagttgcg gattatttgg ttaaaaataa tgttgctttt
    18361 agagatgcac atggtatagt aggaagtatc gttatatatt gtgaagataa taaaaaagca
    18421 atagaagatt taacattaga agaattacaa aagttttcag atgcttttaa ggaagatatt
    18481 tatgatttta tcgactatga gagtatatta aataagggaa taaaaaagaa tttaaaataa
    18541 tgtatttatt tttaaaagct gtaaatttga atgctatcat ttaaatttac agctttttat
    18601 tgtgtaagat tttattatat aaaagtaaga aacttaaaag gagtagtatt taaaataaat
    18661 acaaataaaa aattattatt aaaatgataa ttaatatcaa taagtattga caaaattatt
    18721 tctaaatact attatatata atataaataa taatgaaaat caatatcaaa taaatgggag
    18781 gaaatctaat gaacttatta gtgattggtg gtcatgaaag aatggaaaaa gattatatgg
    18841 ttatggccaa gaaaaaaggt tataagacaa aggtatatac taccatgtca tctaaattaa
    18901 ataattctat aggaagacca gatgcagttg tcatacttac atcaactgtt tcacataaga
    18961 tgtctagaac ggttgagtct caagctaaaa aacaagatat attaatcgta aggcataaaa
    19021 atagtagtaa ggtagcattt aatgaatgtc tagatatggt tgatgaatgt ctgggaaatt
    19081 gttcaaaatg taagcttaaa gacaaacaaa aacaaaaaaa tatataggta taaaaaatac
    19141 ataaacataa aattaaaagt actgagataa tagttataat tattatctca gtacttttag
    19201 atttataagg ataagtatat tataaatttt ttattataat ttccaactac ttgttacttt
    19261 ttcatatttt ttttacttct agcttttttt ctttttttct tctttatatt tgaagctatt
    19321 ataaataaaa ctaaaagcac tagaatacct gcaataatct taacaaaagt cattaaaata
    19381 ttatttccag taaaaaatgg aagactgcta tcaagattat tttttgcaat taagtctata
    19441 ctgttttcta gtttaccatt attatataca tttaaagtac caaccttagc tccttttttt
    19501 ataggcaaat caattttatt taactctgtt ttggtatcat aattgccttg agattcatct
    19561 ttttctaaga atatcctata attatttttt ggttcataaa tcaattcatg ttgttttgta
    19621 aacaatactt ttttagtatc tgtgtatttt tctttatcaa caatagtagc acacttaaag
    19681 ttatcaaatc cataatcaat cagaatacgt gagtctaaat ataaatcagc attagttgaa
    19741 ttaaatacag caacgataac tcttctgcca tcttttacag cactcgataa aagacattta
    19801 ccagcagcat cagtatatcc agtttttata ccatcaacta tatcatattt tatatttata
    19861 ggttgacctt tgtatgtaat cttatcatgt gatgtcaaaa acttattagt gtttacaaag
    19921 tatctctcat aaggatatgc ttttgtagct tcaaatttaa gtgacttagt ttttacaatt
    19981 tgtctaaaag tatcattatt catagcttct ctagctatga gagccatatc atgagctgtt
    20041 gtgtagtgat ttgggtctgg taatccatga ggattattaa aatgagtatt agtagctcca
    20101 attttttttg cctcagagtt catgaggtca ataaattttt gtacattgcc accaccaaca
    20161 tatctagcta atacaaaagc agcatcatta gcagaatgaa ccaaaagagc atctagtaac
    20221 tgctttacag taaaagattc cccatttttt agatacatac tagagccatc tatttgaggt
    20281 aaatctttaa tttctattac ttgatttaaa tctttaacct cctttagaac taaacaagct
    20341 gtccatactt tagttgttga agctggataa agcttagaat ttccatcctt attatatagt
    20401 attttgccag actcatagtc cattaaaact gcatgtttag caactatagc tggttcatta
    20461 tctgcaaaag agatattaga gcttcctaaa aatgaagtaa atatcaataa acagattaat
    20521 aaaagtgata aatttctttt cattgtgagc ctccaaattt taaattcatc ttttagtata
    20581 gcatatattt gtcaataatt aaaagaaagg agtgattaat atatgaaaaa aaaggtaaaa
    20641 tttatttcgt tttttgtttt atttatttta ataatatcat caataatcgt ttataaatcg
    20701 cttattttca aagatgatat ttatgttgta agtaaaaaaa atttacctaa tgaaaaaaca
    20761 catgttgtag agaatgaaat taaaggtgaa gataataatc acaataaagt aactaaagac
    20821 atagataaaa attttcaaaa taataaaata actatataca taagtggagc agtaaataga
    20881 cctggaattg taactataga gtcagacaag agactttatg atgctgtaga attactagga
    20941 ggaacgacaa aagaagcaga tttaaatgga gtcaatctat cagtaagatt agaagatgaa
    21001 caacattaca taatacctaa aataggtgaa gcaacatctg ttacaagtaa tgatgattcc
    21061 aataaaccaa atcagaagaa tgagtcaaga ggtgagccta aaaataaatc caataaaaat
    21121 atatctaacg aatcaaaagt aaatataaat gtagcgacga tagaagagtt agattctcta
    21181 cctggggttg gagaagctac agccaataaa attttacaac acagagaaga aaatggacag
    21241 ttcagttcaa ttgaggaaat aaaaaatgta aatggtattg gagataaaaa gtatgaaaat
    21301 ataaaagatt taatttgtgt agactaaaat ataatacaaa acttacatat agatatgtta
    21361 ttataaatat gtatgtatat gtgtgtacat gtggaaattt agtaaaggag gcacttttat
    21421 gaaaaaagaa caaaaaaaac ttacagagat gacaacagca ggtggttgag ccgctaaaat
    21481 agggccagag gttctggcat cagttttatc tcaattgcct aaaaatgata atatagaaaa
    21541 tttattagta ggactagata cagcagatga tgctgcagtt tataaattaa atgatgatat
    21601 ggcacttatc caaactttag acttctttac accaatggta gatgacccat acgtatttgg
    21661 tcaaattgct gcatcaaatt cactatcaga tgtatatgct atgggaggca aacctttggt
    21721 tgctatgaat attgtttgtt ttccatcatg ccatgatatg gatgtacttg cagaaatatt
    21781 aaaaggtggg tttgataaag tgaaagaaag tggagcactt ttggttggag ggcatactgt
    21841 agatgacaaa gagcctaaat atggtctttc agtttctggg attgtaagtc caaataaagt
    21901 tttatcaaat gctactgcaa aacctggtga taagttaatt ataacaaaac caattggagt
    21961 gggtgtatta aatacagcca tgaaagaagg tatggtagaa caacatatag cagataaagt
    22021 aatagaaata atgatacatt taaataaata tgcagcaatg agttttgaga aatttgatgt
    22081 aaattcggtt acagatataa ctggttttgg tctacttggt catactttag aaatggccaa
    22141 agcttctgaa gtaagtattg aaattgaatc aaaacatgta ccaattattg aaggtgcaat
    22201 agaaatggca caaatgggaa ttatacctgc tggtatgtat aaaaatatgc attatgtatc
    22261 taaagatgta gaagttgtag gaaatataga agttgcagtt caagatattt tatacgaccc
    22321 acaaacatca ggagggttgt taatttcagt aaaagaagaa cttgcagagg aacttgtaaa
    22381 agatatgaaa ttaaatggag caatagaagc taaaattata ggttctgtag ttccaaaagg
    22441 ggaaaaatat attacagttt tataaaatat tatagagaaa gagtgattgt tttgagcaaa
    22501 agagaattgt ttgcaatgtt gccttcagtt gatgaagtat taagtgataa tagaattgta
    22561 gaaataataa atgaatatcc tagaagttta gttttagaat ctgtgagaga agtaatagat
    22621 ttaaaaagac aatttatatt aagattaaaa gaagatgcat caaactctgt aacaatagag
    22681 tttggtgaaa taatagaatc tgcaatagag agagttaagt taaattactc attatcttta
    22741 aaaaaagtta taaatgcaac tggtacagtt attcatacaa atttaggaag gtctttactt
    22801 agtgaagata taaaagatga attatggtgt gctgcttcaa ggtattcaaa cttagaatac
    22861 gatttggata atggagaaag aggttcaaga tactctcatt taactaatac aataaaaaga
    22921 cttactggag cagaagatgt actagttgta aataataatg cagcggcagt acttcttgta
    22981 cttagcacaa tggctaaagg aggagaagca attgtatctc gtggagaatt agttgaagta
    23041 ggtggttcat ttaggattcc aagtataatg gctcttagtg gtgcagaatt agttgaagtt
    23101 ggttcaacta ataaaactca tttgaaagat tacaaggagg ccattacaga agatacaaat
    23161 gtattgatga aggttcatac aagtaattat agaataatgg gattcacaga gagtgtttct
    23221 atagaagaat tggttaattt aggtaaaaaa tataaactac ctgtaataga agacttagga
    23281 agtggagttt ttatagattt atcaaaatat ggtttatctt atgaaccaac tgtattggat
    23341 tctattcgac aaggtgcaga tgtagtaact tttagtggag ataagatgct tggaggtcca
    23401 caagcaggta ttatagttgg gaaaaaagag tatatagaaa aaatgaagaa aaatcaactt
    23461 actagagcac ttagagttga taagcttaca atatgtgctc ttgaagcaac attgagaatg
    23521 tatttagatg agacaaaagc tatagaaaat atacctactt taaaaatgtt gacatataaa
    23581 attgaagaat tagaagtaaa agcaaataaa ctatttgaaa aaataactgc tttaaactta
    23641 aatgcaaaca taaatataga agatggattt tcacaggtgg gaggagggtc tatgcctctt
    23701 gaaactataa gtacaaaggt aatatcaata actcctgaac atatgaatgt ttcatcttta
    23761 gaaaaaaaac taagacttag tgaagcacat ataatagcca gagtgtatga taataaatat
    23821 gtactagatg taagaactat atttgatgat gaatttgatg taatagttga ggaattgaga
    23881 aaagcattta attaaataaa tttgagttaa taaatattaa atttagaagt ataagtatga
    23941 agttttgatt aaaaaatagc tacacacact tgtgacttta gtcatagcag gtttagaggg
    24001 ggcaaaaaat gaaaaatgta gtaattggaa cagcaggtca tattgaccac ggaaaaacta
    24061 ctcttataaa agcattgact ggaagagaga ctgatacatt agatgaagaa aaaaagagag
    24121 gtatttcaat aaacttagga tttacttatt ttgatttacc aaacaataga agagctggaa
    24181 ttgttgatgt cccaggacac gaaaaattta taaaaaacat gttagctgga gcatcaggaa
    24241 ttgatatagt tttacttgtt gtagcagcag atgaaggggt tatgccacag acagttgagc
    24301 atttagatat tctaagcttt ttaaatatca aaaatggaat agtggttcta actaagtctg
    24361 atactgtaga tgatgaattt agagagctag taaaagaaga tatttgtgaa aaaataaaag
    24421 gaacttttct tgaagatgct gaaataattg aagttgattc tatatctaaa agaggtatag
    24481 acactcttat tgaaaaaata gataaaatga gtgatgatat agaagataaa aatgaaaact
    24541 ctccagcaag acttaatata gacagagtat tttcaataaa agggtttggt actgtaataa
    24601 caggtacttt aatagaagga aagataagta tagaagatga tttggttata tatccacagg
    24661 tattaaaaac aaaaataaga agtatacaag ttcatggaga aaataaagat acagcttatg
    24721 caggtcaaag aacagcaatc aatatatcaa atgtaaaagt ggatgatata aaaagaggag
    24781 atgttttagc tgcaccaaac tcattggagg aatcaatgat gttagatgta aaattatcat
    24841 tggtaaatca tttagataaa ggattaaagc actgggatag attaagattg tatcatggaa
    24901 ctagagagat actttgtaga gcagttccac tagataaaga attaattgaa agtggagaaa
    24961 gtggctatgt tcagttgaga cttgaagaaa gcatagtatc taagaaaggt gatacgtttg
    25021 tagtgagacg ttattctcca atggaaacta taggtggagg tgtaataatt gacccatctc
    25081 ctaaaaaaca taagaaattt gatgaaaaag ttatagaagc attaaaaata aaagaaaaag
    25141 gcgaactaaa agatataata gaagagtact taaaaaggaa tttaaaaaat tatccaaata
    25201 taaaagaaat aatgagctat agtggtgctc atgaagaaga tgtaaaaaga gcgcttgaaa
    25261 cacttatatc agaagataaa gtatttatta taggaaatat gtatatgcat ataaatcaat
    25321 acaataaatt aaaagaaaat acaataaagc ttttaagtga gtaccataag aagtataggc
    25381 ttagaaaagg gatactaaaa gaagaagtaa ggtctaagat agagagtaat tttaagacta
    25441 gggagatgga tattttatta gaaaaattgt ctacagaaaa tgctataaaa atagaaaata
    25501 atatagtatc tctattagat tttgaggtta ttttaaatga taagcaaaaa gaaatagcaa
    25561 aaaaaataga aaaaagatta aaatcatgtg gagtttcttc tatactgaca atagatgaag
    25621 tttctgaagg aaatcataac tatgctgaag tattggagtc aatgataggt aataaagtag
    25681 agaaattaga tgatttgtat ataatggaca aagatattta tgaaaatgca aagaacattt
    25741 tgataaatta tataaaagaa aataaagaaa taacattagg agaatacaga gatttgattg
    25801 attctagcag aaagaattgc atgattatat tggaaaactt cgatagaaac aaaataacta
    25861 aaagagtaga aaacaaaaga attttgtttt agcagaaaat gtctgttttt gctttaaggt
    25921 ataaagaagg aattataatg tcgagtatcg tataattaat tagtataaaa tattaattaa
    25981 gaggaagtgt aaagtattga agaatataat acctgatgta aaaccagata taaaagaaag
    26041 agaagacatg agccttttat ttataataat gggctcagta cttttattat ttattataaa
    26101 agatatagag aaatatgaaa ctttattaca tgccatatat acgatatttt tagtagctac
    26161 aggagtaatt atatttaata ctacaaaatt taaagtagat tcattttcta catttttagg
    26221 attaattttt atagctactg gagtattgga gtgtatatat ttatttaatt ccttggggat
    26281 taaaactaag tctataatgg agattaacat cacaatatca gcaataacag atttatttcc
    26341 tatactgggt gtatacttaa gttttaaatt tgttaaggat aacaaacaaa tttattcaag
    26401 tgtaatttta tttatagcaa ctgctacatt aacaataaca tctttattca taatatcagg
    26461 aatttgtgac tatctgggaa atggtaaatc acaatatgct ttaggagtta taatatctat
    26521 attcataata attatttcta tcattagtgg aattgaatta aattcaagtt caaataaagg
    26581 taaatgggaa tatgatgaaa aaaagatttt taacagaata atagtaataa taatcttatc
    26641 aagagttcca aatctattgc atatagttat tgataataga aatgtagaga ggatattatc
    26701 acagataata ataaatgtag ccttatatta tttatataat tacatagtat ctaagaatat
    26761 taagaaaaca gcactggaat tgcatgatac gaatgaagaa ttaacaaaaa aaacggaaag
    26821 tttaaaagaa aagaataaaa aattaatcta tgagacagaa aagatagagg aacttaaggg
    26881 aatacttatg aaaagagaat ctaggctaca atctacatta gatgtagcag taaatagcat
    26941 agtagtattt agcaaagatg gagaaattac atatgcaaat aagagcttta gaaatacttt
    27001 tggagactat aaagaacaag ataaattgac tgataaaata aaaaacttca atgatttgat
    27061 agaaagcatt cattatgtgt ttgttcatga gaagaatgca gagaaactta ttagtacaag
    27121 cgataataaa gtttatcaag ctatatttgc acctttaatt attgcttcac aaacagaagg
    27181 agtattgtgt gtactagaag ataagactaa gaaaaaggag tacgaacgca aacttattga
    27241 agcaaataag agaagtaaag attttttaga aagtattgga gatggcatag tagtgcttga
    27301 aggtaataac aaaatatatg ttaataatgc atgtagggaa atttttaaaa ataatttaga
    27361 gagtattgat ttttcattag tatgtaaaag tgaagaaagt atagaaaaaa gatatgtaat
    27421 agatggtgaa gtaaagtatg tagagatgag tttttctcag tatactaatg ctggtactaa
    27481 taaaactatt attgtaatac gtgatacaac ctctcgaaaa atatctcaaa taaaacttga
    27541 ggaaagtcaa tcatcatatt caagatttat agatatatta cctgatggca tatgcttatt
    27601 aaaagaagat ttaagtataa actatgcaaa taagtcgttt ttagatatgc tagcttttac
    27661 aaacatagat gatataaaag attctaatat taaactatta atgaatgcga atactgaaga
    27721 aaaaatgaaa tttacggata atatggctaa agttttaaat gaaaatagaa gtatactttt
    27781 actggaatat gaattgataa atagttatga tgatatagta gaagtagaat taagtgcatt
    27841 accttttgct atatacaata ctagatacat aatgcttatt ataaaagatt tagtacataa
    27901 gaaatattcc gagcaagcag aaaaagaact gcttgaaaga tttaaaacag ataaaatcaa
    27961 aacggagttt tttgcaaata tgtctcatga actaaaaact ccactaaatg ttatatctag
    28021 ttcaaatcaa ctggtagatt ctttctatag aaatgaaaag ataaaagatt acaataacaa
    28081 tattaaatct catgtggatt tagttagaca aagttcttat aggcttcaaa gattaataaa
    28141 taacattata gatttaacaa agatggagtc tgggttctat acactaaaac ttgcgaaata
    28201 caatattgta tctgtaatag aagacttatt tatgaatata gaagagtatg cacttagaaa
    28261 agacataaaa attctttttg atacagacct tgaagaaatt aatgtttata tagacaaggt
    28321 tgaaattgag agaattatgt tgaatctatt gtcaaactgt attaagttta ctgataatgg
    28381 aggctggata tatgtaagta ttcattataa aatagataaa gtaatcataa gtgtaaaaga
    28441 cactggagtt ggaataccac aagataaatt agaattgatt tttgaagaat tttcacaagt
    28501 tgataagact ttatccagaa atactgaagg aagtggaata gggcttgcta tagttaagaa
    28561 tctagtaagt cttcatggtg gagatataga agtagtaagt gaagtgaata aaggcacaga
    28621 gttcttaatt tcacttccaa tgaagggatt tagtagtgaa cattacacag aagataagag
    28681 aatatataat attcaagaaa aaataaaaat agaattttct gatatatact attaaaaatg
    28741 attatgagca aaaataaaaa tactatttgt ttaaatattt aagttgtaag aaataggcta
    28801 tcataaggta aattattatg atagcctatt tttagctagg ttttatatct atatgtgaga
    28861 acatataatc tctaaattac cttcaaaact aaaatctcca aagtcgtttg gcagtataaa
    28921 atgcataccc ttttttaaaa tatattctac agaattgtgt ataagttttc catttccatc
    28981 caacacactg tataaagaaa aaggtgtatt gtgtgtaaaa ttacattttc caaaaacatc
    29041 taatttataa acactaaaaa attcatttga cacaaatgtt gtgcatttaa agtctgatgt
    29101 tgatacgatt ttatagtcag tatcgaaatt tatatgagga acattcgtaa catctattga
    29161 tttttgaaca tgaagttcac gcttattacc tgaattgtca gttctatcat aatcatagac
    29221 tctataagta gtatctgagt tttgttgagt ttctaaaatc aatgttcctt tgcatatagc
    29281 atgtatagta ccacttggaa catagaaaaa gtctcccttt tttatattaa cctttctaag
    29341 tagtttgtcc cattcattat tatttaccat atttacaaat tctttatgag attttgcatt
    29401 atgacctatt ataatttctg catcatcaga acaatctatt acataccaac actctgtctt
    29461 acctaactct ccattttcat tttttttagc atattcatca tttggatgaa cttgaactga
    29521 taaattatca tttgcatcca atatttttgt aagtaaagga aacttatcac caggtgtgtt
    29581 tccaaataat tctctatttt tattccacaa ttcagatagt tttttacctt tatattttcc
    29641 attttctatt aagcaatctc catttttatg agagcttata gcccaacatt caccagttgt
    29701 tggcgaatct atttcataat taaatttatc tttaagtgca gtaccacccc atattctatc
    29761 cataaaaata ggttttaaaa ataatggttc cataatcctg ttcctcctat gatattaaat
    29821 ttatcctaca aataattata tataaataga ttataaaatc aatagtaaaa aagttttgtc
    29881 ccttgaaatg acataaagta taaggagtta ttaaataaga taaactatga tatataatat
    29941 aaagttctaa attggagtga taattatgga gttgtttaag gcagaaagca aaaaatatga
    30001 aaaagaattt ttaaaattat tggagcaatg ggttagtata ccttcatttt atgatagaaa
    30061 aactgtatct aaagatatgc cttttggaaa aggagtatat gatgcattaa actggtttga
    30121 aaatctaggt agagaaaaca atttcaaagt aaaaaatata gataatcatg ctgttcaaat
    30181 tgaatatgga aatggtaaag agtatgtaga tatatttgga cattgtgatg ttgtaaatcc
    30241 aggtgaagga tgggatagtg aaccatttaa actaaatatt ataggtgata aattagttgc
    30301 tagaggtgta tctgataata aagggcctat gatagtgaat tttttagcac taaaaatgat
    30361 aaaggattta gatattaatt taaaaagaaa ggttagatta atagctgggg gaaatgaaga
    30421 gagtggattt aaatgtataa agcattatta tagcaaagag ccatatggtg tatgtggatt
    30481 tactccagat gctaaatttc ctgtattaaa tggagaaaaa ggtggagcaa taataaagtt
    30541 aatttcaaat atagacgata aatctttata tataagtggt ggaatagagt ttaatacaat
    30601 accagataaa gtttatataa agaatgtaga aaaattgggt aaagataata tatgttttga
    30661 tattaacaat atatcgataa attatgataa tggcaattat attgtgtatg gaaaaggtgg
    30721 tcattcatct aaacccgaaa aatcaataaa tcctatatta gctacaatta aacttttgtc
    30781 agaaaatatt gatgaaaaat ggacaaaaga tttatataag ttgataaatc aagataatat
    30841 aaatggaaac ttatttggtt taaatattga ggggaaatgt gggattttat caatggtgcc
    30901 aacaataata aatatagtag atggaaaatt agaagtagtt ttaagcgtta gatatccaga
    30961 aatattaacc attgaagata taataaaaaa atttaatttg tatatggagc aaaataatat
    31021 taataaattt gagcttattg gggaaaattt aaagcaagca aattatatag atagaaattc
    31081 aaaacttgta agaagtttgc atgatatata tataaagtat tctggagatt taaaaaatga
    31141 tgttagagta actagtgcag gaagttatgc ttctgaaatg aataattcag taatatttgg
    31201 ttgtgaattt cctgatggta gctttggcaa tgtccatagt gcgaatgaat ttgctagctt
    31261 agatagattt ataactgcaa taggtatata tgcagaggca ataattacat tatgtaataa
    31321 aatataaata caaaatgtcc cttatataga catattatat atacaattaa aattttactt
    31381 gatatataat tgaaatcaaa gagaaggtga tacaatgata actaagaaac atgcaataat
    31441 agtaaagagt ttaagcaata aagatggata tatgacatca aacgaattag ctatcaaatt
    31501 ggatgtgtct actaaaacta ttaaaagata tatagcagat ttaaatagtg ttttaagcaa
    31561 gtatgatttg gtaatagctt cttctagagg tataggatac aagttaagcg gttctaaaaa
    31621 caatatagcc agagctatta aagaagcaaa taaatatatt gatggttttt tagatgactc
    31681 tgaagagtca agaatgtcaa atataatatg tatgcttatt aatagaaatt atatgagtat
    31741 agaagcaatg gcagaagaat taaatctaag tatagccgct ataaataaat tgtctagtaa
    31801 gcttaaaaga aaacttgaaa agtatgactt agttataaaa tctaaacctt actatggaag
    31861 ctatatacat ggtgaagaaa ttaatataag gcaattaatt actgactatg caataaaatt
    31921 agatgaaaaa agtaaagtga aggtgttttt agatgacatt agtgaaaacg atatatactg
    31981 tatagaaagt attttagaaa agcacttaag agaaaaagat acaataattt cagataaaga
    32041 ttttaatctt ttgctttcaa aaataatcgt ttctgtattt aggagtaaga gaggtcatag
    32101 taacaatata aacttaatgg atacaagtta tagatttcat aactactact ttatagaaaa
    32161 tctaatgaaa gaaatatctg ataaaattgg atttaaactt attgaagatg aagtaattta
    32221 tatttcaaat tattctggtg taatagccta taaaggtaca caaggaagaa aaaacacaag
    32281 tgaaattgag gaacgaataa gtagtgttat aagtagtgca cttcaagata tattccttat
    32341 ttcaggtagt gattatacaa aagatgatga gtttatggtt gcaatctctg accatataaa
    32401 gagattttta aatagagcta gagctaatgt aaaatcaaat aatcctttac ttcatcaaat
    32461 aaaagagaag tttcctatag cttttaattt agccgtgttt ttatcaaata aactagaaac
    32521 tgagtttagt ttaaaacttg atgaagatga gcttggatat atagctatcc actttgctgc
    32581 ttcaaatgaa agaatgaaaa aaaatactag taagaaaatt tgtatagttt gtcattatgg
    32641 gataggaaca ggacaactta tttctgaaaa gctaaaacag aatataagtg atttaagtgt
    32701 tgtaggtgtt tatccagtta gatatctcga tatggctata agtcaagatg ttgatttgat
    32761 agtatctacc gttgaattaa aaggatatga aaaacctgtc ttatatatag aaaatatatt
    32821 tgatgatagc ttaattgaaa acgtaaacaa agccttttat gaaaaggaag aaagaagaaa
    32881 gataataagt aacatgtttg atgaaaaagc ttttttcagt ataaaggcta gtactaaaga
    32941 agaggtcata atttcacttt caaataaact aaaagaaaga gattttatag aggaaagttc
    33001 tgtaaaaagt ataatagata gagaaaatat ttcatcaact gaaataggaa atctagtagc
    33061 aatacctcac acaatagtga agggagataa aaaatctata ataggagttg gaatacttga
    33121 aaatccaata atttgggata agcaagaggt tcagctagta tttatggtat tttttaatac
    33181 taaagaaaaa cataatttct cgatatttaa atacctttat aattttataa aagatgaagg
    33241 aggagtaaga ggaattatta aaatatgtga ctttaataaa ttaatggcac tgataggtaa
    33301 ttaaggagga aattaataat gggaacaaac atatttaaca aagaatatgt atttttaaat
    33361 gttgatgcta agtcaaaggt tgaagtttta aaatttatat caaaaaaagc taaagactta
    33421 aacttggcag aaggtgagag cttagtatat gaaggtttaa tggctagaga agaacaattt
    33481 actacaaatt taggagaatc tatagcaata cctcacacta aaaatgatgc aattgaaaat
    33541 ccagcagtag tagtattgaa gtttaatgaa gatgttgttt ggaatgaagg tgaggataag
    33601 gttaaattag caataagctt acttatgcca ggaaaaagta aagagaatat tcatcttaaa
    33661 ttattatcat ctctttcaag aaaattaata aataaagaat ttaaagattc tcttttaaaa
    33721 agtgataatg ttgaagaaat atctaactta ataaatgaag ctttaggttt ataattaaac
    33781 actaaggtta taaaataaaa aaataattaa ctaaaaatag gaggaatttg aaatgaataa
    33841 aaaattagta gcattatgtg cttgtccaat gggactagct cacactttca tggcagcaga
    33901 agcaatagag caagcagcaa aggcattagg ttatgaagca aaagttgaaa ctcaaggtgc
    33961 tgatggtgta caaaatgagt taacaagaga tgatatatta ggagcaaata tgataataca
    34021 tgcagtagca ataacaccag aaggtatgga aagatttgat ggttgtgaag tttatgaagt
    34081 tgaattacaa gaagctataa aaaatgcaga aggtgttata aaagaaatag aagaagattt
    34141 aggaatataa taaatttatt gtggaggaaa tagaatggca attaagaaaa aagttataaa
    34201 cagttcaggt gcttctacaa caggaggaaa taatgtagga aaatctactc catctagtcc
    34261 aactcaatca aagggaaatg gaaaatggaa agaaataagt aaacatataa tgacaggtat
    34321 atcatatatg ataccagttt tagtaatggg tggacttata ggggccttat ctcaattaat
    34381 accatatgct atattaggac ttgacccatc tgtaggtata gttgatgcta tgaactcagg
    34441 agaatttaca gggttcaagc ttagtctttt aaatatagca caattaatgt caaatttcgg
    34501 atttactttg tttggatttg cgataccact gtttgcagca ttttgtgcaa actctatagg
    34561 tggaaaaaca gctttaattg caggatttat aggtggatat atagctaata aacctgtagg
    34621 tgtaccacaa ttcgttgatg gacaatggac tgaagttgtt ccagttgcat caggattttt
    34681 aggtgcaata ctaatagcat ttataatagg atattttgta aaatggttaa ataaatctat
    34741 aaaagtttct cataactggt tagcttttaa aacaacattt ttaataccat taatagcgtc
    34801 attagcttgt atggtattga tgatatttat aataactcca tttggtggat taattaatga
    34861 aagtatgaaa aacttcttaa cagcagcagg agcagctgga gaatatgtat atgcaacagc
    34921 tttggcagca gctacagcat tcgacttagg aggaccaata aataaagcag caggattcgt
    34981 tgcacttggt ttaactacag agaatgtact tccaataaca gcaagaacaa tagctatagt
    35041 tgttcctcca attggattag gattaacaac attattagat aaaagattag taggaagaag
    35101 agtatatgat agacaattct atcaagcagg aaaaacttct atattcttat catttatggg
    35161 tatatctgaa ggagcaatac catttgcact tgagagacca ggttttgtta tacctttaaa
    35221 catagttgga agtgtaatag gagctattac aggtataatt ctaggagcaa ttcaatggtt
    35281 cccagagtca gctatatggg catggcctct agtagataac ttatttggat atataatagg
    35341 tatagcagtt ggtgctatat tcatagcagt aggaaatata ttctatagaa ataaattaat
    35401 aaaagatggt aaacttgttg tagattatat cgactagtat ttaattttta ctatctataa
    35461 ataactttgg ctggtaatat ggttttaatc aatattacca gccttaccta tatgattaat
    35521 taaaggcggt ggagataaga tatggataag atagaaaaag taagagagta ttttagagaa
    35581 tatgatatag atggatttct tataaatagt cctacaaaca agttttatgt tggaaattta
    35641 tttagctctt caggttatgt ttttataacg aaagaatcac aatatataat agtagatttt
    35701 agatattttg aagaaattaa aagaaaatct agtttgttta atgtagtatt aatggacaaa
    35761 actagaactc attttgatat aattaatgat atttgtagag aacagaatat aaaggaaatt
    35821 ggatttgagg gaaatgaagt atcttttgat ttatatagaa gtatgagtaa taagttaagt
    35881 gcaaccttaa aatcagttga tttatctaca ttaagggaaa caaaaaatga agacgaaata
    35941 aaatatataa aaaaagcttg tgaaatagtt gatgctacat tttatcatat agtagacttt
    36001 ataaaggttg gtatgactga aaagcaagtt gaaaatgaaa ttgttagaat tataaaagat
    36061 ttaggtggtc aaaaagaatc tttcgataca atagttgcat ctgggcttag aggagcatta
    36121 cctcatggaa aagcaagtga aaaagttata gagtatggag attttgttac ttttgacttt
    36181 ggtgctaagt ataataatta ttgttcagat attaccagaa ccatttgtat gggtactata
    36241 aataaagagt tagaagaaat atataatata gtaagaaaag ctaatgaaga ctgtataagg
    36301 gttttaagac cgggaatgac tactggagag atagataaag ttgcaagaga cataattggt
    36361 tcatatggat atgctggtaa ttttggtcat aatctgggtc atggtgtagg aattatggtt
    36421 catgaatatc ctgcattagc tcctgaatca aatgaagtat taaaagaagg aatgattgta
    36481 actatagagc ctggtatata tgttccaagt cttggaggcg taagaataga agatgatgta
    36541 cttataacac aagatggatg tatgagattg actacatcta ctaaggattt aatagttgta
    36601 agctaaaaat aaattagata aatattttat aacaataaga aaagaagagg taaatttaaa
    36661 tgtataattt tgatgataaa ccagatagag taagtgaaaa gtgtagaaaa tgggatttaa
    36721 atattataag agataaattt ggagatatta gagaagattt tgtaccaatg tggattgctg
    36781 atatggattt taaaatacca acagaaatag aaaacaaatt tattgaagct gtaagaagag
    36841 gtgtatttgg atatacatac tgttatgatg aattttatga ttcagttatt ggttggcaaa
    36901 aaaatatgca tgaggtaaat gtagaaaaag aatggattac attaacttat ggaactgtat
    36961 caacacttca ttatgtagta caagcttttt gtaagaaagg tgatagtata atattaaaca
    37021 caccagttta tgaccccttt gaatcatcag caaaaaaaca aggtgtaaat gtaatttgta
    37081 atacattaga tgttgtaaat gatagatatt atataaattt tgataaatta gaagttcaaa
    37141 ttaaagaaaa taagcctaaa ttaatgatgt tttgtacacc acataatcca tctggaagga
    37201 tatggacaat tgaagaaatg acaagggtag caactatatg taaagaaaat aatgttattt
    37261 tggttgcaga tgaagtacat gcagagcata ttcattatgg taaatttaat tcaatactta
    37321 agattggaaa agagctttta gaaaatgtta ttctattgac atcaccaaat aagggcttta
    37381 atcttggtgg attaaagact tcttattcaa tagttataaa taaggatata agggacaaat
    37441 ttagaaataa attaaagcaa aattctataa cttctccaaa tgtgtttgga ataataggat
    37501 tgataaccgc ttataatgaa tgtcatgagt ggttaagtgg tgtaaatgaa tatataaaat
    37561 caaattatga acttttagaa acttgggtaa ataaatataa taagataaaa ttgatgaaaa
    37621 tggaatcatc ttacttagct tggatggata taagtggatt aggtattagt gcaagtgagt
    37681 ttacagataa attggctata gatacaggag tattattaga agatgggagt cattttgtaa
    37741 aagatggtga gaagtatgtt agaataaatt taggaactca aaaggaaaat gtaattgaag
    37801 ctttaaatag aatggattta tttttaaagt ctttataaaa atttggttga attagtattt
    37861 tatataaatt taaactatta atatggtata tacataagta gaatttgttt tagattactt
    37921 aagtatatac catattttat ataaaaatta aatttgcatt agtcatgtga taactattta
    37981 aattcatatg tattttgtat gtataataga tataattatt atttaaactt attgtttatt
    38041 taatttttca atagaaaaag atttatctgt aagtttaaaa ttatctttta tacccttagt
    38101 taagtatact ttcttatcct tagttatgaa tatagcatct acattatcaa ttttattaat
    38161 taaatccata cctttttcaa gaccaagtcc aaatgtagaa gtggataagg catcacaatt
    38221 aatagattta tcagagatta ttgtaacact acttaaattg ttttcaaaag gataccctgt
    38281 aaatggattt aacatatgat gatatatttt tccgtttttt tcgataaatc tttcataaat
    38341 tccagaagta acaacagatt tatttgtggt ttctatgttt cctatggatt caccattttt
    38401 actagttggg tcttgaatac ctattttaaa aggtttatca tttttaccat tttctaaagt
    38461 aaatatattt cctcctaagt ttactaaacc tttttttaca tcctctgatt ttaaataggc
    38521 tacaatttta tcagcagcat atcctttagc tatggcacct aaatcaattt tcatattttc
    38581 tttggttaat tttatagatt tatttttttc atccaatatg atgttcttat aatcaataag
    38641 aggcagaagt ttttgtatct cagaatcact tggaactcta gctttatctg ttccaatagc
    38701 ccataaattt gataatggac caattgttat gtcaaaatat ccatctgata atttggaata
    38761 ttgtatagct tcttttacga cgaaaaatgt atcatcagaa actctgacaa attttttacc
    38821 agcattatca tttattttag aaacatcact tcctggtata tgagtgctca ttttgttgtc
    38881 aatatatctt aaaattgaat cacattcatt taagatttta tcacttttgg attcttttac
    38941 attaaataca gtaacttcgt ttactgtacc aaggtagtag ctagttttac tatatttagt
    39001 attctggctt gaactcctaa aaaatgaaaa taaaattaat gctaaaatca atatcacaaa
    39061 aataaatgtg atttttttct tattcatggt atattcctcc tatgaaattg tgttattatt
    39121 ttattataac ataatttcat aggaggacaa aaaatgaaga aaaaagacat tcttcttatt
    39181 ttaggcattt tggttgttat tacagcatgt tatgggataa taaatgttat aaattctaaa
    39241 gatgctggaa atattgagat ttatgtagat aataaattgt ataaaactgt ttcaataaat
    39301 gccaaagaag aatttaaaat agagaataga gggggttata acatagtaaa gattcatgat
    39361 aaaggggttg agatagtaga tgcttcatgc cctgataagg tttgcgtaca tactggattt
    39421 ataaacaagc caagtcaaag catagtttgt atacctaata gagtgagcat aaaaataaat
    39481 acaaatgaaa agaatgataa tcaagaagat ataatttcta aataaaataa taaaaataat
    39541 tgaaaggaat tggtgataat tatggttaac ttaggaaacg attgggatga attattgaaa
    39601 gaagaatttg aaaaagatta ctacttaaac ttaagaaaat ttcttataga agaatataaa
    39661 acacgccaaa ttttcccaaa tatgcacaat atatatgaag ctttaaaaca cacttcatac
    39721 aaagacacaa aagtcttaat attaggacaa gacccttatc atggagataa tcaagctcat
    39781 ggtttagctt tttcagttca accacaagtg aaaactccac catctctatt aaatatgtat
    39841 aaagaattaa aagatgattt aggttgtttt atacctaata atggatattt aatgccttgg
    39901 gcagaccaag gagtgttact tttaaatact gctcttactg ttagagcgca tgaagctaat
    39961 tctcataaga acaaaggatg ggaaattttc actgatagag taatatctat tttaagtgag
    40021 cgtgaagacc cagttatttt tgtactatgg ggaagtaatg cacgtaaaaa agtagagttg
    40081 attgatacaa gtaaacatta tattttggaa gcacctcatc caagcccatt gtcagcaagt
    40141 aaaggctttt ttggatgtaa acacttctct aagataaatg aaattttgaa aaagctagga
    40201 aaagaaccta ttaattggca gatagaaaat atataaaagt aaaaggtaac aaaatgataa
    40261 caaaacaaca ataaacaatg acattgttgt aactaataga aatatgctta aatagttgaa
    40321 aatactatgt atgcatattt ctattttttt gtcaagttga caactcttga aaaaatagtt
    40381 acaatttagt tacacaaaaa acacaaataa aaaaagaaac ttacaaagca gaattttatg
    40441 aagcaaaata tatagtgtaa aaaatcttag ggaggaaatt ttatatgaat tttaaaaaat
    40501 tgagtaatgc taaagtctcg gtaatggcag ttgttttatc agttggaata ttatcgggat
    40561 actctgcgct taataaacaa gtaacattag tagtaagggg agaagaaaga actatatcaa
    40621 catttagttc taatgtagga gaccttttag aatctcaaaa cattagctat gatgcaaatg
    40681 atatagtaac tttagatgaa aatacaaagt tatctaatgg agataaaata gaagtaattg
    40741 atgtaaagga aaaaacagtt gtagaaacta aagacattcc atttgaagtt acagttgttg
    40801 aagatggctc tttattaaag ggtgattcta aggttaaaga agaaggaaag tctggtaaaa
    40861 gtgaattaat atataaagaa acttatcaca atggaaagaa agtagaaaag gaatttgtga
    40921 aaaaggttgt taaagctgag ccagtaaata aggttgtaag caaagggaca aaggtagaaa
    40981 tgcaagtagc ctcatcaagg ggagaaagtt caagaagaat ggcttcatca tcatctagta
    41041 gcagtagtag taacaatggt aaaaatatga gagtggtagc aactgcatat actggtcaca
    41101 gtataactgc aacaggaaca aaacctaagt ggggaacaat agctgtagac cctaaggtta
    41161 taccatatgg aactaaagtt tatatacctc aatttgacat ggtgtttact gcagaagact
    41221 gtggtggagc aataaaagga aataaaattg atatctacat gaatgattca aaaacagttt
    41281 ataactgggg aagaaaaact atagatatac gtatattaaa atagttaaat aatttatatt
    41341 tttactggaa attatatttt gattatatag agaagaaact ctttatagat tctaaaaata
    41401 taatttccag ttttttattt tggatatgta ataaaacagg taaattttaa ccatgtatag
    41461 ataaaagcta tttaaattat aatttactat catataaatc tataaaaatt gttgcaaaga
    41521 gtcaaaatat atgatattat agatttcgtt gagaaggatt tcattatggg agtggatggg
    41581 tgccggtgtg ccctctagtc ttcaaaacta gtatgagggg ttaagagcct cttaggtggg
    41641 ttcgattccc acgcattccc gccaatatgt aaaaggtatc ttttaattaa aagatacctt
    41701 tttaaattta ttttaagata atttattttt aataatatat aatgttttgt attatataat
    41761 gtaaattttc taatactatt atatatttag ataaaaatct aggagtgtgg aaagatggaa
    41821 aataatttta caacgagaac tagttttcta gttggagatg atgggataaa aaaacttaat
    41881 aattcaaata taatagtatt tggagttgga ggagttggta gttttactgt tgaagcttta
    41941 gcaagagctg gagtaggcaa tattactatt gtagattttg atgatgtaga tataaccaat
    42001 ataaatagac aaataccagc cctacattca actgtaggaa ggtacaaagt tgatgtcatg
    42061 gaagaaagaa tattagatat aaatcctaat atcaatataa aaaaaataag aagtctttat
    42121 aataaagata ctagtgacga aatactaact gaaagatatg attatgttgt ggatgcgata
    42181 gacatggtaa gttccaaaat acatctaatt gaaacttgtg aaaaaaaagg attgaaaata
    42241 ataagttcta tgggtatggg aaataaatta gacccaacta aaatagtcgt tacagatata
    42301 cacaaaacaa gtacttgtcc tctagctaaa gttatgagga aagaacttag ggatagaggt
    42361 atcaaaaagt taaaagtagt atattctact gaacaaccaa tagaacttaa gaagaaagta
    42421 atgaatggta gaaaagtaac acctggtagt gtatcatttg tcccatctgt aggaggttta
    42481 ataatcgcat ctgtaatagt aaatgaacta ttaggacaat aagttttaaa tattttaatt
    42541 tataaatttt atatacaaat ttgaaggcta gaagcatgaa tataaagcaa ttttatattc
    42601 atacttttag cctagttatt ttatattacc ttattttatg tagttgtagt tttatttttt
    42661 agattatgta tctatttcat aattatattt tgtaaatata atttctccat cactataaaa
    42721 aacaatatct cctattctgt ctgttctata aattgataca tggttttgtt ttagattatt
    42781 aataacctcc ctatgtggat gaccatattg atttttatat ccacatgata ttgcagctat
    42841 atctggacta atttttttta taaattctaa agatgaagaa gaattacttc catgatgacc
    42901 tatttttata aaattaatat tatttaattt aaaagaatgt aaaatttctt tttcattttc
    42961 ttcttcagca tcacccataa ataaaaaaga attatcatta aaagttaatt tgaaaactat
    43021 agaatttaaa ttagattctt cttgtatata tgatggactt aatacatata tatttatatt
    43081 gttatctatg tttaaaacat catttttata gagatgctga atagataagt ttttatccgt
    43141 acaagaattt attagatttt gataagcctc actatcagtt gattgttcag gcatatatat
    43201 ggagtttaca ttaaattttt ttataatatt atcaagactt cctatgtggt ctgaatctgg
    43261 atgagttgct attataatat caatagtttt tattcttttt tgtctaagat aactaataat
    43321 tatattttcg ctatcttcat cacctccatc aattaatata ttcttatttg tgggtgtttg
    43381 aactaaaata ctatcacctt gtccaacatc aatcatatgt atagatagta gagatttttt
    43441 gtcacagccg attaaaagtg atattataat aagtattgat aaaaaaattt tatttctcaa
    43501 taaaaaatca ctccattcat tacatgttta tataaattta tgatgggtaa tataagatat
    43561 atatgactta ttatatttta tattttaaac aatataaatg aaattaatac cataataaaa
    43621 ttggagtttt ttatatatac aaaataaaat ttttaaaatt aagcaataaa aaaggtagga
    43681 ggattttatg tttttcaaag atattgaact aaattcaaaa aaagagttag acccatattt
    43741 tgatttagta gattatgaag cttgtgagta ctgttttagt actttatata tgtggcaaca
    43801 tgtttataag acaggatatt atataggtga agattttgcc gttttagttg gggaatacga
    43861 aggagatagc ttctcaatat taccacttgc taaaaaagat aaacttccag aagttgtaga
    43921 ttttgtcctt gaatattttt ctaaaaataa taaaaaaata tacttaagag gtattactac
    43981 tgaagtagta gagtttttaa aagaaaaata tccaggtaga tttgagtata tagaagaaag
    44041 agatttattt gactatattt atgatgcaga aagtttaaga actctagctg gtaaaaagaa
    44101 tcaaaagaag agaaaccata taaactattt cttaaaagag tatgcaggta gatatgaagc
    44161 taaattatta gataaagaaa actttgatga gtgtttagta ctaatgaagg aatgggaaag
    44221 taataaggaa gaaaataatg aatttgatga aagtatggat gatgaattaa taggtatcaa
    44281 aaagatattt aaccattatg acatattaaa agataaggta aaagtgtttg gtgtttatgt
    44341 agatggaaaa ttagaagctt ttagtatagg agagctttta aatccaaata tggcattgat
    44401 tcatatagaa aaagctaatc ccgatataag aggtctgtat ccatttataa atcaacaatt
    44461 tttggtaagt gaatttaagg acgttgagtt tgtaaataga gaagaagatt taggtattga
    44521 aggattaaga aaagctaaac tttcttatca tccttgcaga tttgttgaaa agtacagtgt
    44581 tagggaggct taatagacta tatggagatt aggtatgcta aagaagaaga gatagaaagt
    44641 ataaaagaga tttggagtta ttgttttaac gatacggaga gttttatgaa gtactatttt
    44701 aatgataaat ataaatctga aaatactgta gtagctttag atgaaggaaa aattatttct
    44761 tctcttcagc taaatcagta taagttatta ctaaatagca aggtatataa tacatcatat
    44821 gtagtaggag tttctacact tccagaggga agaggagctg gatatatgaa caaagtaatg
    44881 aaatttactt taaacgaatt gtataaaaaa gggcaattag tttctatatt gatgcctatt
    44941 gactatagac tttatagaag atttggatat gagcattgtt acgaccagat tgaatatact
    45001 attaacacag atgatttaaa aaattttaag tcaagtggaa aaatgataaa gtctaattta
    45061 tcacaaattg atgatttgat acaaatagat agaacttttt taaatgaggt taatggtaat
    45121 gtgttaaaag atgaacatta ttatgaaaac ttatttaaag agattcaaag tgaagatgga
    45181 tttttataca ttcatgaagg aaatgaaaaa gatggatata tagtttattt tcttcaggaa
    45241 gataaaatgt ttgtcagaga gttattttat aaaaatatag atgcccttaa atcaatgctt
    45301 aaatttatat ataatcacaa tactcaatgt aagatagtta ctatatctac tcctacaata
    45361 gataagataa ggtttatatt agacaaccct aaagatagtg atataaaaat aaaacctttt
    45421 atgatgggaa gagttataaa tgtaaagaag tttattgaag atatagatat tgagaatgat
    45481 attaatagct catttaattt attaatagaa gacaagttta tagatgaaaa taatggtcta
    45541 tttaaaattt ctatacagaa taagaaggta tctgtagagc agttagataa aaaaggtgca
    45601 gaaaagcctc aagaagattt tgatattaaa ttagatataa ataccttaac acagttatca
    45661 ttttcttata tagatgtaaa tgaagctatt ttcttaaatg atataaaata tgtttcagaa
    45721 gaaacactag aaacacttaa ttgtattttt tctaaaa
//
LOCUS       DAEMHW010000009        42799 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEMHW010000009 DAEMHW010000000
VERSION     DAEMHW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.009"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            106..780
                     /locus_tag="KPK67_000390"
     CDS             106..780
                     /locus_tag="KPK67_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2457399.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARISAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            768..1928
                     /locus_tag="KPK67_000391"
     CDS             768..1928
                     /locus_tag="KPK67_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2457400.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTDKLNDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            2143..2670
                     /locus_tag="KPK67_000392"
     CDS             2143..2670
                     /locus_tag="KPK67_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBG2457401.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LVARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            2956..3624
                     /locus_tag="KPK67_000393"
     CDS             2956..3624
                     /locus_tag="KPK67_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2457402.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKGYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3627..6095
                     /locus_tag="KPK67_000394"
     CDS             3627..6095
                     /locus_tag="KPK67_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2457403.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIRKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(6335..7759)
                     /locus_tag="KPK67_000395"
     CDS             complement(6335..7759)
                     /locus_tag="KPK67_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2457404.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIHGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7778..8470)
                     /locus_tag="KPK67_000396"
     CDS             complement(7778..8470)
                     /locus_tag="KPK67_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2457405.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELLKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8522..9301)
                     /locus_tag="KPK67_000397"
     CDS             complement(8522..9301)
                     /locus_tag="KPK67_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG2457406.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            complement(9298..10068)
                     /locus_tag="KPK67_000398"
     CDS             complement(9298..10068)
                     /locus_tag="KPK67_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG2457407.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIFVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTVLEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10065..10769)
                     /locus_tag="KPK67_000399"
     CDS             complement(10065..10769)
                     /locus_tag="KPK67_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG2457408.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(11024..11536)
                     /locus_tag="KPK67_000400"
     CDS             complement(11024..11536)
                     /locus_tag="KPK67_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457409.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            11862..12119
                     /locus_tag="KPK67_000401"
     CDS             11862..12119
                     /locus_tag="KPK67_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457410.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            12262..12888
                     /locus_tag="KPK67_000402"
     CDS             12262..12888
                     /locus_tag="KPK67_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457411.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIEVSSINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13138..13917)
                     /locus_tag="KPK67_000403"
     CDS             complement(13138..13917)
                     /locus_tag="KPK67_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG2457412.1"
                     /translation="MNSKIYYFSGTGNSLVVSKILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(13985..14446)
                     /locus_tag="KPK67_000404"
     CDS             complement(13985..14446)
                     /locus_tag="KPK67_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2457413.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(14422..15099)
                     /locus_tag="KPK67_000405"
     CDS             complement(14422..15099)
                     /locus_tag="KPK67_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2457414.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            15332..15718
                     /locus_tag="KPK67_000406"
     CDS             15332..15718
                     /locus_tag="KPK67_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2457415.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            15721..17520
                     /locus_tag="KPK67_000407"
     CDS             15721..17520
                     /locus_tag="KPK67_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBG2457416.1"
                     /translation="MYAPFFIRFLLSTLTLSILIAVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNNPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKNLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(17875..19749)
                     /locus_tag="KPK67_000408"
     CDS             complement(17875..19749)
                     /locus_tag="KPK67_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2457417.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEGENEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIISPISGKLETVFSSGHAFGIKGINGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(19754..21418)
                     /locus_tag="KPK67_000409"
     CDS             complement(19754..21418)
                     /locus_tag="KPK67_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBG2457418.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESEDVIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKAR"
     gene            complement(21432..22253)
                     /locus_tag="KPK67_000410"
     CDS             complement(21432..22253)
                     /locus_tag="KPK67_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2457419.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESTKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(22352..23116)
                     /locus_tag="KPK67_000411"
     CDS             complement(22352..23116)
                     /locus_tag="KPK67_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2457420.1"
                     /translation="MKIERFFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(23113..23862)
                     /locus_tag="KPK67_000412"
     CDS             complement(23113..23862)
                     /locus_tag="KPK67_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2457421.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(24144..25196)
                     /locus_tag="KPK67_000413"
     CDS             complement(24144..25196)
                     /locus_tag="KPK67_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2457422.1"
                     /translation="MNVINVFNSNLKRNINKRIVFLVTLLFPIIIVVLGVLANYISKP
                     SFNIGILDEYKNECILKESEKRTYENTIMALENTQGINTEIADFKTIKTDIITGKYSA
                     VICFNKNGFELYSIKDKNTNDTLKYLIETYKKDPVPVDISKLQENTLGVLQRIIAFIV
                     LFLMITSTVTASMIIKDKNSGTFTRVLYSPQNIRGYVLGNMLYNFTITYFQYIVSISI
                     IKLFNIDIGMDYIDLILMGVWISALATSFGTFVSSLFNKEMHANLFSTCISLILSLIG
                     GSFISVEKMPTMLQKISIISPTQWFISFVTYIEERDGSFFNMNYICILTFIIIILVLL
                     AMKFTQRNKYSSNRNY"
     gene            complement(25196..25936)
                     /locus_tag="KPK67_000414"
     CDS             complement(25196..25936)
                     /locus_tag="KPK67_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2457423.1"
                     /translation="MRCFLTLFKLGIKRRSKDFFILFYNIVFPVIIIVLLGYLTSKSY
                     GKEFTSYNYYTIVTIPFCVLMGIISVSYVAQDEKISNTSYRYMISPISIVDLVISKLF
                     SCTTVFSIYNLITLVVLRGIFHMSFGGKFLVVIFFLMCESIAVVSMGLFLGLVCKNLD
                     MLRNIINIPIVIFGFLGGVFFPVSSLNPVISLIINISPLTWINRGIVACIYDNNLQLL
                     FNISCIFVAVSIFMTILTIKFFKKEVFI"
     gene            complement(25941..26699)
                     /locus_tag="KPK67_000415"
     CDS             complement(25941..26699)
                     /locus_tag="KPK67_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2457424.1"
                     /translation="MSNVLLEIKNLSKKYNNKEVVSRESFKLLEGDILGFIGPNGAGK
                     STSINMLTTIVSPDSGSIYYKGKDIKNQKNIFKSSLGVVPQELAIYEDLSAYDNVKFF
                     CSLYGFKGNELKERTKYALEFVGLWESHKEFPSKFSGGMKRRLNIACAIAHSPQILIM
                     DEPTVGIDPQSRNKILEAIKGINNNGTTVIYTSHYMEEIETLCNRIVLIDKGVIIEDL
                     DKSLYKNKYLKFGCKTLEDIFLYLTGTDLRDLEE"
     gene            complement(26804..27526)
                     /locus_tag="KPK67_000416"
     CDS             complement(26804..27526)
                     /locus_tag="KPK67_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2457425.1"
                     /translation="MIRTLILYYLNIKPTHGYEIQKFLQVSGADRWTKIQSGSIYYAL
                     AKLEKDGGVRVLKEEKTGARIRKIYEITQSGKLELREEIQKELQMPIVPTGSNKFLLH
                     NILDVLPKDTLQKNLEKHIKYLIEQKKYWENWKEIKKIDKKSLATEKIAFDMTIDNLN
                     YQILWHEEILNNIDKYISVGCETQNIIKSIDFSNIEEDFLFTSDTTNELLEVQRLRDE
                     IINNPDKAIENIDKIILKLQNK"
     gene            complement(27627..28232)
                     /locus_tag="KPK67_000417"
     CDS             complement(27627..28232)
                     /locus_tag="KPK67_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2457426.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            28411..29049
                     /locus_tag="KPK67_000418"
     CDS             28411..29049
                     /locus_tag="KPK67_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2457427.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVK
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            29181..29843
                     /locus_tag="KPK67_000419"
     CDS             29181..29843
                     /locus_tag="KPK67_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2457428.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            29843..31024
                     /locus_tag="KPK67_000420"
     CDS             29843..31024
                     /locus_tag="KPK67_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2457429.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNLKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     ANQISRLERLVSSLLTLSKLDASTLIFESKPIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(31205..33865)
                     /locus_tag="KPK67_000421"
     CDS             complement(31205..33865)
                     /locus_tag="KPK67_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2457430.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIGNFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGCRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMMIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(33862..34545)
                     /locus_tag="KPK67_000422"
     CDS             complement(33862..34545)
                     /locus_tag="KPK67_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2457431.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(34889..35827)
                     /gene="blaCDD"
                     /locus_tag="KPK67_000423"
     CDS             complement(34889..35827)
                     /gene="blaCDD"
                     /locus_tag="KPK67_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG2457432.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(35855..36571)
                     /locus_tag="KPK67_000424"
     CDS             complement(35855..36571)
                     /locus_tag="KPK67_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457433.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSESVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGNRNLVYFFFGAELQSIDGVVIFKDLKHVKPKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            36903..39146
                     /locus_tag="KPK67_000425"
     CDS             36903..39146
                     /locus_tag="KPK67_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG2457434.1"
                     /translation="MSNIIIKGLTQNNLKNISFKIPKNKIVVFTGVSGSGKSSIVFDI
                     IAAESQRQMNETYSSFIRNRLPKYRKPQVELIDNLTASVIVDQTPLGSNARSTVGTIS
                     DLYSSLRLLFSRIGEPYIGTTSYFSFNDPNGMCKNCSGIGRVKVINIESIINPENSWN
                     TGCINDSLFKPNSWYWKQYAESGLFDLDKPIKDYSFEEYNLLLYGSKNKDGKQENPKI
                     EGIFNKYNQTYLNRDISNMSKHTKEKSERLITDKECPVCHGKRLNKETLNCNILGYSI
                     ADMCNMELTDLYDLLLQIKYKRTETVIKTLTDGLKRMIDIGLPYLHLNRESSSLSGGE
                     AQRLKLVRYMGSSLTGMTYIFDEPSAGMHPRDVHRMNQLLKKLRDRGNTVLVVEHDKD
                     VISIADEVIDIGPSAGTQGGHVVFQGSYDKLLLANTLTSNAMNLSLPIKTDSRHPKGT
                     LPIRDACINNLKHIDVDLPVGIMTVVTGVAGSGKSTLISRVFAQKYEKDVIKIDQSPV
                     TATNRSMPATFLGFFDEIRKVFAKENNVEEGLFSFNSKGACPVCDGKGVLVTELVFMD
                     PIINTCEACKGTRYSEEVLSKLYKGKCIVDVLSMTVDESLEFFTEKKLCKILQTMKDV
                     GLPYMTLGQPLSTLSGGERQRIKLAKNLNKKGTIYILDEPTTGLHGSDIEKLMLIFEH
                     IVDKGNTVIIIEHNLDVLKQADWVIDIGPDGGKNGGQFVFEGTPLEMFERGCTITSDF
                     LRKSINI"
     gene            complement(39408..41522)
                     /locus_tag="KPK67_000426"
     CDS             complement(39408..41522)
                     /locus_tag="KPK67_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2457435.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRDEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMVEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYVGTLTQLISM"
     gene            complement(41780..42640)
                     /locus_tag="KPK67_000427"
     CDS             complement(41780..42640)
                     /locus_tag="KPK67_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2457436.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
BASE COUNT        15103 a         6800 c         5244 g        15652 t
ORIGIN      
        1 ttaatgatta agttttgaaa tataactaag aatttgataa tttattgaga tttttatact
       61 aaaatataca tactatacaa acttaattta gaagggagca ttcttatgaa caataaaata
      121 ctcgtcgtag atgatgagga aggtataata acactcttaa aagattactt tgaaatgaat
      181 ggttatgagg tttacactgc tcaaagtgga aaagacgcat taaaaaaaat atccaaaaat
      241 ccagatatca ttctacttga tataaacatg cctgaaatag atggaattga agtttgtaat
      301 agaattagag attatgtatc atgccctatt ctatttctta cagctagaat tgaaaatact
      361 gacaagataa atggttttcg tgctggtgct gatgattata ttgtaaaacc ttttgattta
      421 gacgaacttg gagctagaat atctgcccat ttaagacgtg aacaaaggaa gtcaaatcaa
      481 actaaaatta aattttataa tgatttaaca atagattacg aaaaaaggtt aatttgtata
      541 tataataatc agatttcact ttcaaaaaag gaatttgata tagtagagct tctctctatg
      601 aatcctggac aggtatttga taaagaaaaa atatatgata gagtctgggg atacaatagc
      661 aatggaaata gtgatgttat aatggaacac attagaaaaa tacgaatcaa actatcaaaa
      721 catactcttg aaaattatat agaaacagtc tggggagttg gttataaatg gataaaataa
      781 ataatatgtc attaaaaaaa gctttctttg ttcttacttt atcttcacta attatagcat
      841 cagtacttac tactttcgca tacattttac ttaataatct atataattct atacaagata
      901 agtatttaaa aacacctata aatacaggta ttataaccat agttcaaaac aatgtgaacc
      961 aatttagtga ttatgataaa acattactta atataatcaa catacttcag cttgtacttc
     1021 cacttatatt ttttatagga cttttattat tagctgatat aattttttac aaggtaaaat
     1081 taaaaacacc catagaaata ctcaataaag gtgcaatgga aatatccaac aacaatttag
     1141 acttttgctt agagtataat aataatgatg aattaggtaa tctatgtaat gcttttgaaa
     1201 aaatgaggtc tgaactaaat aaaaacaaca taaagatgtg gactatgatt aatgatagaa
     1261 aacaacttaa tgctgctttt tctcatgatt taagaaatcc cctaacagtt ctaaaaggtt
     1321 actcaaacta tttaacaaaa tacataccaa cagataagct taatgatgag aagattttat
     1381 caaccacaca acttatgtct gagcatatta acagaattga atactatgta gaaaatatga
     1441 gtaatgctca acgattagaa gatttagttg tttcaaaatc tatgtcgaat ataaacaagt
     1501 ttatagaaaa cttagatgaa aatatatcta tcattgctaa acaagaagga aaatctttta
     1561 cacttaaaag tcaaataaat aatataaatc tattttttga tgaaaatatc attcttagag
     1621 tcgtcgaaaa tataatatcg aatgctttta gatatgctag aaataatgta tccatactta
     1681 tatatcttga acaagaactc cttacctttg taattgaaga tgatggtata ggatttagta
     1741 aggaatctct aaaattagct ttaagacctt tctatagaga taagacttta aatgacagca
     1801 atgcacactt tggtatgggt ctttatatct caaaaatatt atgtgaaaaa catggtggct
     1861 ctatttctat tgaaaataat tctactaaca gtgctaaaat tactgctaaa ttctcaacaa
     1921 gaaattaaaa tgattttgta agctttcact gttcttatat aagctttgtt aatttagaat
     1981 ttataaaatg tatttcaatt tacaaccaat atatactatt ttgtagaagt tgtaagagtt
     2041 gcttcaatta taatattttg gcaaaaggca tttgaaacaa taatagaaat atatatttaa
     2101 atatgtagaa tagtatgaaa ttagaatttt ggaggtgatt atattaaaag ttttgctaat
     2161 aatattacat tctctcgtat tgtgtttgca ttgctaatgc ttagttctac attctgttca
     2221 ctgcctttct ggatatgtta tttttggtgt ggaattagtg atatacttga tggtcttgtt
     2281 gcccgaaagt tgagacaaca aagtggaata ggtgcaaagt tagatagtat tgctgatttc
     2341 atttttgcgg ttacaatttt ggttactgtc ggaaaaaata ttgctcttcc catgtatcta
     2401 tggtggtgta ttgttgcaat agcattgtta cggttgatta gttacagtat tggttttttt
     2461 aagtatcgca cattttcatc gcttcatact ttgatgaata aaatcactgg aatttctatc
     2521 tttgcttttc cattactcta tatttgcttt ggtcttgatg ttgcaggaat tatgatatgt
     2581 atcgtggctt ttctttcttc ttttgaagaa atagcaatta tgataaaatc aaaagaatta
     2641 aatcgtgata taaaaagtat atttatgtga caacttagaa tttgtgacaa aaataattta
     2701 tataataatg aggaagtaaa acataaaaaa ttatccacaa gtttatgtgt aaaatagttt
     2761 taaaacaata gagttgtatt gtaaagcctt tagtgcataa attacatttt aaattaaaaa
     2821 tgatactgaa actgcttatc agcactgcga attatccgca aatagtgaag actttttaga
     2881 tttaagctaa aagttgggaa aaaattgagt tttaaaactt acactcttct tatatagaaa
     2941 aggagagtgt tttttatgat tataaaagca aaacaattat caaaaatata tggttctaat
     3001 aacaataaag ttatcgcact taataatgta aatttagaaa taaactcagg tgaatttgtt
     3061 tctgtaattg gaccttctgg aagtggaaaa agcacacttt tacatatttt aagtggatta
     3121 gataatccta cctctggaca agtgttactt gatgataaag atatatataa acacactgaa
     3181 aaagaactat ctgcacttag aagaaaatcc tttggattcg tctttcaaca atttaatctt
     3241 ttacctgttc ttacagcatc tgaaaatatt tcaatgcctg tacttctaga taaaaaacaa
     3301 cctgataaag gctatttaaa tgaaatttca tcattacttg gaattgcaga tagactcaat
     3361 catcttcccc atgaactttc aggcggtcag caacaaagag ttgcaattgc tagagcactt
     3421 attgcaaaac cagatataat atttgctgat gagcctacag gaaacttgga cagtaaaagt
     3481 ggcagtgaag ttatgaatct tcttataaaa acttcaaaac agtttggaaa gactttagtg
     3541 gtaattactc atgatgatag aattgcaaaa cttgctgata gaaaaatttc aattattgat
     3601 ggtgttctta tggaggtgaa atagaaatga agaattacct ttcactttca ataaaagaat
     3661 taaaaagcca gaaacttatg actacattca tcattatagc aatagtacta tcaactatta
     3721 tgacaactgt ggttggacag tcaattggta tcctacaaaa tttaagaatt gaacaagccc
     3781 gttcttttaa tggtgataga catgtatcct ttcaccaact cactaaaaat caagttgatg
     3841 acataaggaa agatgataga gtttatcaag ctggtacttc aataactatt ggcagtagta
     3901 aaataaagga ttctggtatc tctgtacttg taaaagagta tgataaaaca ggactttcta
     3961 actacccaaa attaatgaaa ttaaaatctg gacatttgcc taaagataaa aacgaaatag
     4021 ccttagatga aaacacacta aagcttatgg gtataaaacc aagactagga gtgactatac
     4081 ctatgaattt agatatttca cttttaaatg ataccatacc accatataat tacacagcta
     4141 actttaaatt gagtggcatt ttagaggatg attatacagg atatgtaagt ggtatagtaa
     4201 atggtattgt aggtaaaggt acatcagaaa atctattacc taagagatat attttatcct
     4261 ctttggattt taaaataaaa cagcaagaaa agtttcaaga aatagtaaat caattagcta
     4321 aaaaaataaa tctttctcac aattcaattc aatataattg gatttactta aatgctctta
     4381 agatacagtt tgaaaaagat gaaaatagtt ctaatagtga tggtatatct atgattatat
     4441 tggtatcttt atttgttgcc ttattggttt tactggcttc tggacttgta atttataata
     4501 ttttaaaaat ctctgttaca aagaaaatta aagagtatgg atgtttaaga gcaataggag
     4561 ctgaaccaaa ccagatatat aagatagtta ttttacaaat cttaatttta tgtactgttg
     4621 ccatacctat tggagctgta attggaatta tatcttctaa aggcataact ggaatggtca
     4681 ctaatatctt aaatccagat atacttctag caaatgataa taaagaaatt actgagctta
     4741 tacataaaaa tacaacagca tatatgttcc cattagtatt aagcactaat gtatctttga
     4801 ttttttcatt tatttcagca ttaccatcag caatatatgc ttcacatgtc tctcccaaaa
     4861 ttgctatggt tggtagtact accaaaatca aaagaaaaat taaaagagaa aaaacaataa
     4921 aaaactttga aagacatcta gcatggctaa acttaaaaag aaataaagga agaactataa
     4981 taactatact atcattattt atgagtataa cagtattcgt tgcactgagt gagttctcta
     5041 atgtattaga tgtatctagg tcagtaagta atttaaaaga aggagatttt tccttaacaa
     5101 atgaaatatc tggttttgat aaatcaacac tagataaaat agctaaaatg aaaaacgtca
     5161 atcgcacttc ctttataaag tactctgaat acaaacaggg ggaaatagat acagatataa
     5221 attttgaaaa tagtggtgag atgttaaaaa tcattggaat tgatgagcaa actttaaaag
     5281 acttaatgcc ttctattaca gattctatac tagaagactt taaaaatggt tctatctgtt
     5341 ttataaaaaa tccgattgct atttctacac caggtgtaaa aactagacat acaaatttaa
     5401 aacctaaaga tagtatcaca atcaacaaaa aacaactaga tatatatagt acagtagata
     5461 aaatgttctt tttacaaggt aatggctgga taaatggtgt tgatgttatt gtatatgatt
     5521 ctgtttataa caccttgaca aataaaaaca agttcaacca gataaatatt tatgctaaag
     5581 ataaatcaaa attagaacaa ataagattgt ctattgaaca gatttgtgaa aataatcctg
     5641 gctctcattg gatatcatat attgaatcag ataaacaact taaagagagt tttaaacaaa
     5701 tagaatttct agcatgggca gttatattat ttgtgggatt gattggtact ttaaacatca
     5761 ttaacacaac tcatacaaat ataaatacta ggactaatga gattggagtt aagagagcta
     5821 ttggtatgag taatagtagc ttatataaaa tgtttttatg ggaaggtgtt tattatggaa
     5881 tttttgctgc tatatttggt tcaattgctg ggtatgccag tgctattatt ataaacatgg
     5941 caactataga aaagcttgat ttcactaata taccaattac ttcaatttta caagctacta
     6001 ttatttctgt acttgcatgt attatagcta ctcttattcc gttaagaaaa gttaaaaaga
     6061 tgaatataat tgattgtata gatatcaatt tataactatt ttacagataa taatataaat
     6121 taaattatca atgactgtct ctattttctt tcaaatctaa ctaataaatc tttttaacta
     6181 taatataagc tttaaaaaag gcctgtctca aaatagagat aagccttttt tcattgtcga
     6241 aataatgaaa atatatttaa cttcacaaaa aaagagtgcc tcacgaatta aaaatttcgc
     6301 tttgagacac tcccctttaa tttaaaatat atagttaaat gctatgccaa aatttcacac
     6361 atgctccacc tgattttgaa ttttctattt taacagaacc tccaagctgt tccactaatt
     6421 gtttgacaat gtaaagccca agaccagaat gtccaccttt tgtctgccta gcttcatctc
     6481 ctcgataaaa cttatcaaga gcctttttta aatctttaga gctaaaccct ctaccagaat
     6541 cacaaatctc ataagaaata caattttttt catcctttac agtaatacta atattaccac
     6601 ctgatggagt gtattgcaga ctgttggata tgatattatc aaaaattcgt gtcaacctat
     6661 ccacgtcgat ttgaattggg gattgtatat tcccctgcat tttcaatata agttcaactt
     6721 ccttttgatg agcctgtaat tcatagtctt gcaccttttg cctcaaaaat tcaggtaaat
     6781 ttataggaac aagatttaat tgtatatttg acttttctaa gtcagacgta tattgcatct
     6841 tttggacaag agcagcactt ttttctgtat tttctcttat cactgtaaga tacctatgaa
     6901 gtttttcatt gtcatctgta ttattaccaa taagtgcatc tgtatatccc aaaataatag
     6961 aaagaggaga ttttaaatca tgtgctagag cttccaccat ttcaacacgt tcctgctcca
     7021 tcttccactg cgcagataaa gaacctttta attcgtcttt catttcagaa aatgcactac
     7081 aaagtttacc gagttcatta tctgaataat agtcgatttc aaaatcaagg tccttttctt
     7141 ttatcttctt ggacgcatca atcaatagat gtaatggttg attgatattc tttacaaagc
     7201 gcttagaaag ccatctagta aaacccacga tatatagaaa tggagaaaac agagccatta
     7261 taataactgc aaatacaaat ctccctctaa tatttgcaaa ggtgattttc acttggtaga
     7321 atagaataac agcaccctca atctttccat tatccccttt gattggaaca gtatggatat
     7381 aaccaccttt acgaacagtt ttattgataa aattattaaa taattcttct tttgttttaa
     7441 atagtttttt ggggttagta ccatatagaa tattgccatt attatccaca acttgatata
     7501 gcatatcatc cccatgaatc gttcttttta aaccttcttc attcgattgt gataacaaag
     7561 ttatgttttt ttcattgatg tatttttcaa ttctaggaac ttgtcgctca taatagtttg
     7621 gtggatagat atccttattt aaggaatgaa tatacaaata gagagcaaat acataagtaa
     7681 tcactgtggc aactatactg gctattataa taaaaataaa agtatgtcta aattgggtaa
     7741 taagggattg atttttcatt taataacctc tctctcttta cattttattc cacttatacc
     7801 caatacccca aactgtggaa atatattctt tttctggtgt cacagcagaa aacttagctc
     7861 gtattttttt tactcgttca acaacagttg aggaatcacc ttcagaatca cacccccata
     7921 ctttttcata gatttgttct cttgagaaaa cttgtcctgc atggagtgct agtaattcaa
     7981 caatatcata ctctattttt gttaaatcaa tattcttacc atcaatttta attgcacgtt
     8041 ccttcatata taagcataac ttcccaaaat acaaagtaga tctcttgttc tcttcattga
     8101 tatactgtga acgcttttcc ctacgcagat tagcttcaat ccttgccata agttcacgca
     8161 gtccaaatgg ctttgttaca taatcatccc ctccaaaggt caaccctttg attttgtcgg
     8221 tttctgattg ctttgcactt aaaaatataa ttgggcataa aacatcatct ctaattgcct
     8281 gacatacctc aaatccattc attttaggca tcataatatc cagtataata aggtcaggct
     8341 cctgttttaa taactctata cctgcctgac catcataagc tatcaacact tcatgtcctt
     8401 ttgccttcaa ttcatcctca agtagcatta ctaggtccat ttcatcatca ataatcaata
     8461 ttttactcaa tataacacca cctttctctt atgggaaggt cagtattaaa acaatattcg
     8521 tctactcata acttttcctt ccctcccaca ttttaaacca aataacgcct cctatcaaga
     8581 atataatcaa acctattgct actactataa gctctatact agctgtttgc atcactttag
     8641 agtaaaatgc ctcagttgaa atagttccag cttctttaat aaaataaatt cctggaagca
     8701 tacctagttt tactggccat gtccatggga caaatgccca tactttttca ccaacgctag
     8761 ttaatccaag tattgctgcc atcaaaattc caccgatact aataccaata gatgcaccca
     8821 taccccatgc aaaactcacc caaagatgaa tagctaatag tggaagtgtc ccaatcacca
     8881 caagtcctgc tgctgacagg aataaccata ttgttgctcc agatggcact gcaatactca
     8941 ttcctatgca aagtatcaac gtagcaataa atgtgcatat agttaggcaa aacagaagaa
     9001 aaagaaattt tcccagatat aatctaacac gagaaatacc tacacataaa aaaccattaa
     9061 aatttccagc aagctcttcc tcctgcacta taaatcctgc taaaatacct actccaatag
     9121 gaatgatgaa tacagtccat actgtaaaaa atccttcaaa agcaaattct tgtgtacttc
     9181 cagatttagt tgtgagataa aaaaccacac ataatgaaaa aataataggc atacaaagag
     9241 taagccactg gacagctgtc ctctttgctc gaagccattc tgatgataat agttttatca
     9301 tttgtcatcc ctcctattga accaagctgc agtaagtgct gttaatacta taaacacaac
     9361 caaagaaaca acaatcccaa taggaatcac tgaagtattc aaaagtgggt ctccagcttc
     9421 taatacagta ccattaggat gtatgttgat gattgggcat accattctaa ctgcccaact
     9481 ccaaggtact gcaatccaaa atggttttgt cgcagcaaga acaccaaata tcatacctaa
     9541 agctccaact cccatactta ggaacatacc tttccatgta gctagccata gctgaagtgg
     9601 aagtatagca agagaaacaa cccagcatac aatacttgcc cctataatct gtccaaaagg
     9661 aactggacca gctttcaata ttaaaccagt tataattgtt acgaatatta aaactagcgt
     9721 tgaaataaaa ctgtagactg ccatagctat aactttatta atccaaagtg tcataggaga
     9781 cacattgtgt gtacgtaaag cacgataatt tccagctttt ttttcctgga aagctaccaa
     9841 agtagcaaac aatgcaaatc caataggcag aaataaaaat gaccaccagt taaaaattaa
     9901 tgctaaaaga ttttgccacg cccaagaagt tgtttgagat aatggatttt gcatgagtgt
     9961 tgattgcatt ataaatgcat atgctgcaaa agacactggg aaaaaaacaa taagtttacc
    10021 cataaatgtt cttttatact ttaaaagttc tgattgtaaa gcattcatct gttttgtcct
    10081 cctttaagaa taacatctgt aaaaaattct tctagatttt cattagggtc aggcattcct
    10141 tgaaataata gtcgtcctcc atttataatt cctatattat ctacaacttg agcaacctct
    10201 gatagaatat ggctagaaag aattacagtt ataccttttt ttggaaaaga agcaatcaat
    10261 tcacgcaatt cttgaatgcc aaaggggtca agaccattgg ttggttcatc tagtattaac
    10321 aactctgggt cactcaaaag tgcaatagct attcctaacc tttgtttcat acccatagaa
    10381 aattgtgaag ctctcttttt acctgtattt ttcaaattta ctatttccaa cacttcataa
    10441 attttatctt taggtattcc caatattttt gtatgtacta acaaattttc ctctgctgta
    10501 aggtttccat acaatgcagg ggattctatt aaagaaccta tttttgcaag agattctcgt
    10561 ttccaaggct ttctatcaaa aataatacga ccatttgttg ggcgaagaag accaacaagc
    10621 attttaagtg ttgtggactt tcctgctcca tttggtccta aaagtccata tatactattt
    10681 cgttcaattt gtagagaaac attgttaact gccatttgtt ctccatatct ttttgataag
    10741 ttttttgttt caagaattaa atctttcata ttaatttctc ccttcttgta ataccttaaa
    10801 tctattacta agagaatact aacatgaaaa aataacaatt ctataacacc tactaatttt
    10861 aataaaaatt aaaataaaaa atagagttac caattttatt ttggcaactc tatttaagtt
    10921 tatgttttac agttatatta gatttaaact tagtttcatt atcacactaa cttcaaattt
    10981 actctataat ccttaaatca aacactaatt tattaatcaa acactaatct attaatttat
    11041 atttctttag acatctaata tagtttgcta ctaaataata attatttata tcatatttat
    11101 ctaatccttt aaaagaagtt atttcatcat taagctcatc actaccttct acaaaaacac
    11161 aacctgtttc aaattccata cctacaataa tcttacagcc actataaatt tcctcttgag
    11221 tatcgtacaa tttttttgca tttccttcat ttggaattgt aaatacaata ggttttaatt
    11281 ctttatcact ggaaatcttt tgtgaaaaag gcttgtttaa cttaatatta ttgataacta
    11341 catgtttaaa ttcctctgaa gtttcttcta ctattacata ttttcgcttt aaaaactcta
    11401 ttatttctaa aacagactta gaatttactt ttaaaatact tttatatttt tcatataaag
    11461 atttccactg ttcaattttt tcctgtggta cttcttggat atgtgtactt attttttctt
    11521 gagatatttt tttcaattaa actctccttt caatacttaa atattcttat aaatctcaca
    11581 tgactaaagt tacaaggttt ctacttaata gaattttgca tacttaaata tgtcttatta
    11641 attctctaca ggatatctcc gtagttccta cgatttttac ataagttact tattttaaat
    11701 tgttattagt tagattctaa aaattattta ttttatcact aatataatac tcttaaaaac
    11761 tttacaaatc aatttaaatt tgctaccttt tatttataac aacttatagt acatttctcc
    11821 tatgcttttc aatatattac ttttaaaagg aggttttcat tatgatttta aatagagctg
    11881 agcttttccg aggatattct ttaaatagat ttaacgagat atgcaatata ttatcagtaa
    11941 ataaaattaa atataagtat aaagtgaaaa ataatgctta ctctaaaaaa ccttttttta
    12001 atgaaataac acttggcaat ttaggtcaaa aggaagattt ctcttatgag tacttaattt
    12061 atgttcataa agacgattat gaacaggcag agtctctaat aaattctaag ttgcgataga
    12121 ttatttataa aacatgacat tctgttgtca tgttttatat gttattatac tttcaaatat
    12181 gctactatat tttcaaaatt gtgtgcttta aaactagctt tttatagtaa tttaaagctt
    12241 aaaactaagg agactcgtta tatgaaaata aatataaaat caaagttaag tgaatttatt
    12301 aaacagaaca atctttttga tgacagcaga ataatatcct accttcctaa tataacaata
    12361 gaaacagaca aaataaaaac tataatgatt aatgaggttg tcccaactaa caccaatgac
    12421 gatttttata gtgtagataa agatgctgat tatttaaaaa ctactattcc tttatttgac
    12481 agtgcaggaa tagaagtatc tagcataaat gatatcctag atatgggtat ctatataact
    12541 aatgcagtaa aacttcctaa atcagaatat acaataactc gtgatacaat taaattacat
    12601 atgcctattt tagaagaaga aattaaatta tttaaaaacc tagaggtagt aatgttaatg
    12661 ggagatgttg caaaaaaatc ttttaatatg attactaaaa agcatataaa aaagaatgtc
    12721 atcccttcca tttcaacata taaacttcgt aataatgaac tttattatga taatttaaga
    12781 gtatttccat catatattat gactggtgga aacatactga ttgaaaaatc aaaatttgaa
    12841 atggcatcag aagatataaa aaaaatgttc gaaattataa acggttgata ttacaaaata
    12901 ataaaaacta atttagccaa ataataagtg cctcaaaaac ttctatttat gaggcacttt
    12961 tattttatat tagtgtaact atatactatt agcataattt attatttata ttgttctttt
    13021 taatctctat tccatttagt ctaggtaaca ttactcttta caagattact ttatatttct
    13081 tttatatctc taacattttt atttaataaa gcaattatta atacaagtca attatatcta
    13141 tttatctaaa aatatatctt tcacagtaat atctggatgg tgatacttct tcctattcgt
    13201 taaaaaagat ttttttacat taatagcctc ttttggacac cagtgaaaac aagctaggca
    13261 tctttcacac tcattacccc actttggctt tttatcaact atacttatat tctctacagg
    13321 acaaaccctt ttacatgtcc cacatccatt acatttattt tctgcaaaaa atttagtagt
    13381 tgcaatttgc atattcttac ctactaaacg aagtacaaca ctatctatat gtgactttaa
    13441 attgtttttt ccttctatat ttccaatctc caagttattg atacattttg caatctcagc
    13501 taccctatta gtctgatttt tcaatctttc ttcttcaaca tctggaggtg ttatcaaagc
    13561 atttccaggc atattgatta aaaaaccagc agataatttt ttcccccttt ttttaagtaa
    13621 cttatctata gtaaataaac tatgtccagc taccccatta caagtggcaa tggcatatat
    13681 atgagtatta atattaaaat ttaatttctc tacaaaagac tttactatat caggaacatc
    13741 agcaaaataa acaggaaaaa caattaccaa tatatcttca tttatgtcta tattattttc
    13801 ttttctatgt atagctaaag gtataacttc tttacttcca tttaacgttt cttttaaaat
    13861 ctttgaaact actagcgaat ttcctgttcc agaaaaataa taaatcttac tattcattct
    13921 tatccctctt tctatatttc tacatcatca atagttaaaa agtctcttaa aataggattt
    13981 atatttactt tccaattcta tccaaaatat cagtaaacaa aggcaattct tcatcagaga
    14041 tagatttaaa tgtctctgtt gcatatatac ttgcaatttc atttaatggt tcttctaatt
    14101 ctctacctaa atctgttagg aatatttgaa acttacgtct atctttaaca tctactttac
    14161 ggacaattaa tcccttattt ttcataacat ctaagattct tgtcgtatta gatttatcct
    14221 tacatgattt ttcacataaa tctatctgag aaattccatc ttgctcccat agaaacctta
    14281 ataacgcata atggtcagtt gttatatcat atccattttg tttaaacatc ttttttaaat
    14341 catcttttac ttttaacgaa gtacttacta acttagttgt agcttcatat agtttctcaa
    14401 aatctttcat tttacctcca tttaaataat cccttgctcc tcccatctat caattattcc
    14461 cttatattgc tcaatatctt taaaagccat tggctttaaa acagcattta tagcaataaa
    14521 attatagatt tgagaaagca caggtggatt tttcttttcc tctatgcaat aaataaattt
    14581 tttagatacc ttttgtatct ttgattctac tttttcagtt ataggatttt gattaaatcc
    14641 agtttttata gataaactac cacaaacata acctcccgaa tctaagacca tggattttat
    14701 aaaagaaatt gcttttgaac cacgtgcatt ttcatatgta gtaactgcta tacatggctt
    14761 acgatacaat aatctttcaa gcatcatatg tgctctatct gtaaacacct tgaaaagtcc
    14821 agttacacta cttccataag ttgggctacc aaatataaca ccatcactct tagtaattat
    14881 atcatgaata tactctactt tatcattttg attgatacac ttgcccattt tataacaatt
    14941 taaacaacca atacaatatg atggatttac ctcacttaaa tcaaaataat taatttttac
    15001 atctggtatt aatctttcta tatctttata caagtaagtt aaaacttttg atgtagctcc
    15061 attttttcta gggcttccat ttataactgt aattatcatt tctatatctc ctttttagtt
    15121 gttatatcaa caataagtat atcaactata tttttagctg tcaatataaa ccatttattt
    15181 aaagctatac ttacacagct taaataaaag ttttatttaa ctacatgtgc ttatttaatc
    15241 aactataaat gaattatttt taataattga caaactaaat taatgataat atactttatg
    15301 tattacaaat gtaagtagaa ggaggtatag tatgaaaaag ttaccacata tttcagaagc
    15361 agaatatcaa gttatgaaaa ttatttggaa atatgctcct atcagcacta ctgaagtaat
    15421 tgaaaagttg gtagaaacaa gtacatggag tcctaaaaca attcaaacaa tgttattgag
    15481 attagttaaa aaaggagctt taacatatga aaaaaatagc agagtattcg tttatactcc
    15541 acttgttaaa gaagaagaat acgtagctac agaaagtagt tcttttttaa atcgttttta
    15601 taatggtact cttaattcta tggtactaaa ctttttagaa aatgataaac tatctgaaga
    15661 tgatattgag gaactgagag aaatcttaaa taaaaggacg actaaggggg ataaataaat
    15721 atgtatgccc cattctttat acgattttta ctcagtacat taaccttatc aatattaatt
    15781 gctgtaattt tacttgccaa aaaagtattt aaaaaacata tttctgcaaa gtaccaatac
    15841 aatattggat ttatattgct tataatgtta ataattccat ttgtccctct taaatatatt
    15901 aatcttggaa atgtatttaa ctatttatat acttttaatc aacttggaag cagtactaat
    15961 aggacttcta tgataaaaaa taatccaaat tctataatta ataactcaaa tgcattaaac
    16021 gatttttcag tatccctaaa ccaagttaat tttgatagtt taaatatttt attactaata
    16081 tcatggataa ttggaatttt aatcatgata gctattacta ttcattgcaa cattaaaata
    16141 aaaaatatga aaaaatcaat acaaatttta agagatgagg agatagtatt tatatttgaa
    16201 aagtgtaaaa aagatattgg tataaataag gattttatac tatgcaaatc acctattata
    16261 aatacaccta ttacatttgg atactttaaa ccacacatta tattaccaga taagtctata
    16321 tctaaattat ctttaaaaga tataaaatat atcttacttc atgaattaca acactttaaa
    16381 aataaagata tactgataaa ttatataatg tgcttccttc aaatattata ttggtttaat
    16441 cctcttgtat ggtatgcatt taaagaaatg cgtatagacc gtgaaattgc atgtgatatt
    16501 tcagttttaa aaaatcttga taaagatagt catatagaat atggacaaac tctaataaac
    16561 tttgtggata atatttcaag accatcaaat cttacactta catcagatat tggtgggtca
    16621 aaaaaacaaa taaaaaagcg actattgaag ataatggatt ttcataatga atcaaatacg
    16681 ttaaaattca aaagcatttt aatttttaca ttgatagctt taattgtatt tggaaatatt
    16741 cccaaactat ctattatggc agatatcaat gaaaaatata gacctacaaa tgaacaaact
    16801 gtgtacgagg acttaagtca gtattttaaa ggttttgatg gaagttttgt tatgtatgat
    16861 ttaaaatcca agcaatatca tatttacaat aaagaaaaaa gtaaaacaag aatttctcca
    16921 aattctactt ataagattta tagtgcctta ttgggattgg aaaatgggat aataacaaga
    16981 aataattcac atatcccttg ggatggaaag catcattcta atagttcttg gaatgaagac
    17041 caagacttat tttctgctat ggaaaactcc gtaaactggt actttcagac tctagataaa
    17101 aaaacagggc taaaaaacat acaaacttat cttaggcaaa ttggatatgg caattatgat
    17161 gtttcaggtg gtatctcaaa cttttggcta gaatcttcat taaaaatatc acctattgaa
    17221 caggtagaat tacttgaaag tttttataca aataagttta aatttgaaga taaaaatata
    17281 gaaacagtaa aagattcatt gttaatatca aaaaaggatg gggtatcact ttttggtaaa
    17341 acaggcactg ctaatataaa tggaaaagat gtaaatggtt ggtttatagg ttatgtggaa
    17401 aaaaatgaaa atacttattt ctttgctaca aatattcaaa gcaaagatta ttctaatggt
    17461 agttcagcat ctaagataac attatctata ttaaatgaca aaaaaatcta taatccttaa
    17521 tatatttact gtacattcta aaaaattcac ataattttca tattaattat cgattaaatt
    17581 catatacaag gcttattata taaacataga agatagtaaa actagcttaa aaacataata
    17641 taatacccta tacaaatagt ggcaactctc cctcacatcc tcaattgttg ccactattta
    17701 attttttgta tgaagttaaa tatattttca tcatttcaac aatttgggtg caatacaatc
    17761 tctattttga aacaacctct ttaattgttt tagagtttat gaattatttc tcatacttta
    17821 aaaagattga attactcttg ataatcttct tttgtagtca ttacatataa cctactactc
    17881 aacacatatt ctttgaccta tttttacatc cttgtttata tccaaaatat ttaccttctt
    17941 accatctgga aacaatacca ttgttaaagt agactttcct aattcgtgta ttctctttaa
    18001 atcaacatta acaagaacat ccccctgttt aaccatatct ccttgtttaa cagcaacatc
    18061 aaagccatct ccatttaatg caactgtatc aattccaaca tgaattaata cttctccatt
    18121 aattccttta atcccaaatg catgtccact tgaaaatact gtttctaatt tacctgaaat
    18181 aggagaaata atttttccat ctataatatc aatagcaaaa ccatcaccta acattttttt
    18241 agaaaatgtt tcatctggta cattttctaa tgcaacaatt ttccctttag ctggagacac
    18301 aaagttttca ttattttcat taaacactaa ttcttcgttt tcaccttcat ttatagcttc
    18361 tttaggcact ccaaataaac atgttacaat aaagcagaat gcaaccacaa atattgatac
    18421 taataagtat gtgattaaat tatatccatc atagatatac attaaataag aaggtaaaac
    18481 tgctaatcca tatgaattag attgaatatg taaaattgat aatattgctg ctcctaaacc
    18541 tgaagatgca cacataataa tcaaaggctt taaattatat cgaagctgaa tcccaaataa
    18601 aacaggctca cttataccaa aaagttgaga tagcattgct ccaactgcag tttgtttaac
    18661 ttgattattt ttagattttt tcataaatgc tagacatgtt ccaatattcg caaatccata
    18721 cattgcacaa agagtaataa gggcattaaa tcctgtattt gctaatagtg atgtttcaat
    18781 cattacataa gtatgatgta agccagtaat aacagctaat ggatatgtaa atccaataat
    18841 aaatcctcca attcctaaag gcaaatcaat caataagctg atgatatcaa ccatttttaa
    18901 ttctattgta tgcattatag ggcctacacc taacaaaata actaacattg ttattagcat
    18961 aacaaagaat ggtgtgaaaa taaggtctaa tacattaggc atatgtttac ggaaccattt
    19021 ttcaagatta gctccaataa atgctgtaac aatagctgtt aaaacacttc cttgacaacc
    19081 tacaatagga ataaatccaa atgccataat tgcttcaatt tcatttgatg ggtcagcaac
    19141 agaatatgca ttaggaagta ttggggaaac taacataagt ccaataacaa gtccaataac
    19201 aggtgttcct ccaaatactt taaatgctga ccatacaata attgctggta aaaaagcaaa
    19261 tgctgtttct gtaagaaccg aaacaagtgt ctgaatatac aatggaatat ttgcagaaga
    19321 cattccaaat aatccaagta cattatcatt aaacaaacaa ccctttaatc ccaagaataa
    19381 tcctgttgct gcaataactg gaataatagg aacaaatata tctgctagag ttctcataac
    19441 tttcttaaat ccagtttcat tatttttaac taattcatct tgttcttttt ttgataaagt
    19501 ttttaatccc attttttcaa cttctgcata aactttatta actattcctg ttcctaaaat
    19561 aatttgatac tgtccagaag taaagaaaac ccctttaacc tcatcaactt tttcaacttt
    19621 cttatcatta ataatttctc tatctttaac catcactctt aatcttgtgg cacaatgtgt
    19681 aatagaaata atattatctt caccaataat tttaataagc tcatttgcta ttttattata
    19741 tttattcatt tttctacctc gctttcaata atactgcatg aaatggtttc aaacgcactg
    19801 ttttatttaa tagatttata tcatctaaat tttggaatac aatatcatat ttttcttcta
    19861 ctcttatttc tacaggttta tcagaaaaat tataataaca atcaataact accaaatcta
    19921 attgccttct atacgcaata acatcttcac tttctatatc aataggttct attgaaccat
    19981 aaatcaaaca atcagaatac tcactatctt gtctcaactt tatcatcttt ttataaaatt
    20041 ctaaaatact ctctgaattg ttaatctgac tctgtgcatt aatatcttta taattttcaa
    20101 taacttttaa ccatggtgtt gtatctgaaa aacctgcatt ttctttatct gcccatggaa
    20161 atggcgttct tgcattatct cggcttctta aattcacaaa ttctaacgct tcttcttctg
    20221 agtaaccttc atctattgaa cgatagtatt ggtcaatgct agataaatca ttaaaatcat
    20281 ttatggtatc tcttttaaaa tttgtcattc ctaattcctg tccctgataa ataaatgggg
    20341 ttcctcttaa aaagaaatac attgctccca acatttttac tgcatcatca ttcctttcgt
    20401 attgcaaaag atactttgtt gtagctcttg gttggtcatg attttcaata aaattagctg
    20461 cccatccatg ttcctgaaca tacatttgac tatctaaaat gcattttctt aaatctggaa
    20521 ttgtccatga aattctttta aaccactcac taccagaagc aacatctaag tcagcatgtt
    20581 taaagtcaaa gaccattgaa aagaaaccat cttctccaat aaaatcacct agttggtcat
    20641 agctaatacc tgctgcttca gcaacagtca tacaatcata ttttttaaac gtattttctt
    20701 ttaattcata aagcattttt tctattccag gctgatttct aactgttttt gtacatttaa
    20761 caagaccatc aacaccatca ctttctaaac tttgaaaact taaattcttt ttgatgaatg
    20821 taatagcatc aactctaaat cctgccactc ccatttgaga ccaggtatta atcatttggt
    20881 aaagttcact tcttaatatt ggattttccc aattaaggtc aggttgtttt tttccaaaag
    20941 aatgaaaata atattcgtct ctattctcta ccttttccca aacacttccg ccaaaaacag
    21001 acctccagtt atttggcaaa tcgtttcctt ttttaaaaat ataataatca tgataaggac
    21061 tttctggatt ttttaatgct tcttgaaacc aaggatgttc atcacttgta tgattaagaa
    21121 ctaaatctaa tataattttg attcctctat ctttactttc tttaattaac tcttttaaat
    21181 cttccatact tccaaattca cttgcaatat ccaaataatc actaatatca tacccattat
    21241 cgtccatagg tgatttaaaa attggacata accacaatga tgtaatacct aaatctttta
    21301 aataatctaa acgagaaata attccctgta aatctccaat tccatcatta ttactatctt
    21361 gaaagcttct tggataaatt tgataaacga tttctttttg ccaccatttc ttttccataa
    21421 attactcctt tttatatgat atttcttctt tttaattctt tataaattcc attatcgaat
    21481 atgtcctcac atatagatgt cgctatatct ttaagttgtt gatgactatt tttcattgca
    21541 attgtaacgt ctgatgcttg aaacatgaca atgtcatttt gaccatcacc aaaacatatg
    21601 gtttcttttt gagttactcc taatctttct tgtaatctct taattgcttt gcccttatga
    21661 aagtcttttt gaataatttc ataatactga ctagaaatat ctctctgtgc taattccatt
    21721 ttatcttgta gtatatcttt cacttcgtca aatacctttt catttgacca taaacaaatt
    21781 ttatgtatat cttgtgactt atactcttca atgttatttt catatgttat tttttcttgg
    21841 atatgttgtt tatttataca agaattagtt cctttcaact gattcattgt ttcaaatatt
    21901 tctttggctt tttgattcat gaaaactttt tcctgactct caatcgaaaa agcaacttct
    21961 ctctttttta ataaacaaac aacttcctta atcaatcttt gattaaaaga ttgattataa
    22021 agtaattcac catgatattg aatgtaatta cctcctcctg caatatatcc atcaacccct
    22081 aatgataaaa catcatcttg tattgtaccc atacttcttc cagtacaaat aacaacactg
    22141 caatgattct tttgacataa acgtatagca tgctttgtag attctgggat tccatatacc
    22201 tcatctctaa gcgtaccatc tatatcaagt attaataatt tgtacaattt catcttcctt
    22261 tcttactatc aaaatcataa tagcattcaa aaaaaaagat atcaatattt tgatatcttt
    22321 taaaaaatat gttcattttt agcacaaatt ttcattttcg gggaatatgt tcactttcta
    22381 aatacatctt gtatttaata taaagcagtt caattaaaat aaaataaggt gttgttacat
    22441 cgtagtccag ttgtttatct acagaaatag atatagattg aatatacagg ttttcatcac
    22501 ataatcttgc taaaggatta cttatcattt ttgtaataga aaccatataa attcctttca
    22561 ttctcatttc tttagcaaaa tcaataattg tagatgcatc tccattcaat gaaataaaga
    22621 aaatcatatc atctgttttt ataaaatgat taagtgcctg aatcatatca taaccataaa
    22681 catcatatat aatttttcct gttggtaaga aaatcctttt catctcttta gcaactcttt
    22741 cctgagaata accttctcca aagacgatta tacgatttgc ctttttcatt ctttcaaaca
    22801 taatcgaaca atccttcttc tctatatcat ctatcacctt attataacaa tccattaatt
    22861 ggtgtttact ttgattttta tattctactt ctgtttcatt taagcgtata ataactctta
    22921 attcactata tccaggtaat tgtagttttt gagcaaaacg agaaagtgca cttgtagata
    22981 tatggtattt gtctgcaaat tcatctatag aaagtttaat acaatcttct ttatgattaa
    23041 gaatacactc acagatatac ttatcatttg ctgtaaatga attataatat ttattgaaaa
    23101 atctttcaat tttcattctt ttctccttct atactctatg taattcatat gaagcaaatc
    23161 taatagagca taaaatgctc ctacgacttc ataagatata gcttgtaatg tatcaagttt
    23221 ttgcgtttca acatacagat tatactcaca aagtgttgca attgtattat tatcaagcct
    23281 agttaaagaa atcgtatgta tatgattgtt gataaatttt gcaatttcaa ttcctgcttt
    23341 tgtttctcca gacaaagaaa tgataataaa taaatcactt ttttgaaatg attctctcat
    23401 aaattcaatt tcacctatat caaataagtc aataacacat ttacctactg aaagaaatat
    23461 tctctttatt tcttgagcta aacttttctg ttcattacct gtcccataaa tataaattgt
    23521 ttctgcatca tatattattt tacaaatatc ttgaaatgat attttttgta attcattcat
    23581 taatagtcga taattctcgc aaacatcatg aaaattatta tcattattaa caaattctag
    23641 ttgattattt ttaagtaaat actttaaatc agagaatgaa tctaatccaa tttttctaca
    23701 aagccttaat aaagtagctc tagaaacatt acattcattt gcaatttttt cacacgaaaa
    23761 attacggtat tctctttgac aaatcaatat ttgtttaata ataattttat cattttcatt
    23821 taaattatca tagttttggt taactaacga ttctaatctc attttttcca ctccctaaat
    23881 attattttaa tatacattag ttgctttttc tagggaagag tcttaaaaat atttctatta
    23941 agacaaataa aaaagagcta tctcagaaca aatttaattt gctcatacca cagttctatt
    24001 ataaataaaa aatttttcac attatttttt acactatata atttctattt ttataattaa
    24061 acccaattta taatatatag aaccatattt atatttttat ctcctgattt gttttaatta
    24121 catctattca ttttctatct tatttaataa ttcctattac ttgaatattt atttctttga
    24181 gtaaatttca tagctaacaa aactaaaatt attattataa atgtcaaaat acaaatataa
    24241 ttcatattaa aaaagcttcc atctctttct tctatataag tcacaaaact aataaaccat
    24301 tgtgttgggc ttataatact aattttttgt aacattgttg gcattttttc aactgaaata
    24361 aatgaaccac caataagtga taatataagt gatatacatg tagaaaacaa atttgcatgc
    24421 atttctttat tgaataaaga agatacaaat gttccaaaag aagttgccaa agctgatatc
    24481 catactccca ttaaaattaa atctatataa tccataccaa tatcaatatt aaataactta
    24541 attatagata ttgaaactat gtactgaaaa tacgttattg taaagttgta taacatatta
    24601 cctagcacat atcctcttat attttgtgga gaatatagca ctcttgtaaa tgtacctgag
    24661 tttttatctt taataatcat tgatgctgtt acagtagatg ttatcattaa aaataatacg
    24721 ataaatgcaa ttattctttg taaaactcct aaagtatttt cttgtaattt acttatatct
    24781 acaggaactg ggtctttttt atatgtttct attaaatact ttaatgtatc attcgtattt
    24841 ttatctttta tagaatataa ctcaaaacca ttcttattaa aacatatgac agctgaatac
    24901 ttgcctgtta taatatcagt tttaattgtt ttaaaatctg caatctctgt attaatccct
    24961 tgagtatttt ctaaagccat tattgtattt tcataggttc ttttttcaga ttcctttaga
    25021 atgcattcgt ttttgtactc atctaaaata ccaatattaa aagatggttt acttatgtaa
    25081 tttgccaata ctcctaaaac tactataatt ataggaaata aaagtgtaac taaaaacact
    25141 attctcttat ttatatttct ttttaagtta ctattaaaaa catttattac attcactata
    25201 taaagacctc ctttttaaaa aatttgattg taagtattgt cataaatata cttacagcta
    25261 caaatatgca tgatatatta aaaaggagtt gtaaattatt atcatagata caagcaacta
    25321 tgcctctatt aatccatgtt aatggtgata tattaatgat taatgaaata actggattca
    25381 atgaactaac tggaaagaac actcctccta agaagccaaa aataactatc ggtatattaa
    25441 tgatattacg taacatatct aaatttttac aaacaagacc caaaaacaac cccatagata
    25501 ctactgcaat agattcacac attaaaaaga aaataacaac taaaaatttc ccaccaaaac
    25561 tcatatgaaa aattcctctt aacaccacta aagttatcaa attgtatatc gaaaatactg
    25621 ttgtgcaaga aaaaagtttt gaaataacta aatctactat tgaaatagga gaaatcatat
    25681 aacgatatga tgtgttagaa attttttcat cttgtgctac atatgaaaca ctaataattc
    25741 ccataagaac acaaaatggt attgtaacaa tagtataata attatatgat gtaaattcct
    25801 taccataaga ctttgatgtc aaatatccta atagtactat gattattact ggaaaaacaa
    25861 tattgtaaaa cagaataaaa aaatctttac ttcttcgttt tattcctaat ttaaacaatg
    25921 ttaaaaaaca tctcatctat ttattcctcc aaatctctca aatctgtacc tgtaagatat
    25981 agaaaaatat cttctaatgt cttacaacca aattttaaat atttattttt atataaactc
    26041 ttgtctaaat cttctataat tactccttta tctattaaaa caattctatt acataaagtt
    26101 tcaatttcct ccatatagtg gctagtatat atgactgttg tgccattatt attaatgcct
    26161 ttaattgctt ccaaaatttt atttcttgat tgagggtcta tacctactgt tggctcatcc
    26221 atgatgagaa tctgtggact atgtgcaatt gcacaagcta tgtttagtct acgtttcatt
    26281 ccaccagaaa attttgatgg aaactcctta tggctttccc aaagaccaac aaattccaag
    26341 gcatatttag ttctctcttt aagttcattc cctttaaatc catataatga gcaaaaaaat
    26401 ttaacattat catatgcact taaatcttca tatattgcta attcttgtgg tacaactcca
    26461 agtgagcttt taaaaatatt tttttgattc ttaatatctt ttcctttata gtatatgctt
    26521 ccactgtctg gagacacaat cgtagtaagc atattgatag atgtgctttt ccctgctcca
    26581 tttggtccaa taaaacctaa aatatctcct tccaataact taaaactttc tcttgataca
    26641 acttccttat tgttatactt cttagataaa tttttaatct ctaacaatac attactcatt
    26701 tataaaatcc tcctaatatt taaccaacat tatatatcca atattgtata tatagttttt
    26761 ttaaaacacg aatgttttac tcgtgttatt ataataaaat ttattactta ttttgtaatt
    26821 tcagtattat cttatctata ttttctatag ctttatctgg attattaatt atttcatctc
    26881 taagtctttg aacttctaat aactcatttg tcgtatcaga tgtaaataaa aaatcttctt
    26941 ctatatttga aaagtctata gatttaatta tattctgtgt ctcgcaacca acagatatat
    27001 acttatcgat attatttaat atttcttcat gccacaaaat ttgataattc agattatcta
    27061 tagtcatatc aaatgcaatt ttttctgttg caagactttt tttatcaatt tttttaatct
    27121 ctttccaatt ttcccaatac ttcttctgct ctataagata ttttatatgt ttttcaagat
    27181 ttttttgcaa agtatcctta ggtaatacat ccaaaatatt atgtaatagg aatttatttg
    27241 aaccagttgg aactattggc atctgaagct ctttttgtat ttcttccctt aattctaatt
    27301 taccgctttg agtaatctca tagattttac gaatcctagc tcctgttttt tcttctttta
    27361 aaactctaac tcctccatcc ttttctaatt ttgccaatgc ataatatatt gacccagatt
    27421 gtattttagt ccatctatct gcaccagata cttgtaaaaa cttttgaatc tcatacccat
    27481 gtgttggctt aatatttaga taatataaaa ttaaagtacg tatcataaaa attcaccacc
    27541 tatactctat cttggatatt atattatcac caacattgga tatagtcaat agataacata
    27601 ggatatatta caacttatta tttttattat ttaatacaag gagttccatg agacatacat
    27661 atccattcat attttaaatt atccaattct cttatagatt tcatcatctt tttataatcc
    27721 caattccatg gattaggata aggaactata tttccattct tatttttgac taaatcacct
    27781 gcaaataata catcttcaaa aatcatgcag acatgccctg gagtatgacc aggagtatgg
    27841 ataattttta tattcccaat ctgcatattc tctccataag gttcaacatc cttaatgagc
    27901 cttctggaaa ttgcctttcc aataaacttc ttaaatccag gtctatcctt ctctcctata
    27961 ataaaaggta tatcctctac atgagcccat acttttgctc ctgttttttc tttcaataat
    28021 ttaatattag atatatggtc aacatcatga tgagttaata aaatatgctt aatatctgta
    28081 agtttaatgc caagtgtttt taattcttta attatatttt tccccataaa aggaagacct
    28141 gtgtctataa gaataatttc tttatcaaac acaataaaac aatttgagcc acgagtacta
    28201 tctaacatta atacattttc ttttatcttc ataaataaat tccccttatt tggtatttta
    28261 cctatataat taattaataa agttataaat agttataata cttgatatta attttatttt
    28321 ttattaaagt atactatagg tatacaagta ttatatttac acaagtatac catcagtata
    28381 cttttttatc aatagaaagg aataatttat atgtcagaat tatcaaaaag agataaagaa
    28441 aaaatacaaa gagaaaatga gattattgat aaggcagaaa aattattttg tcttaatgga
    28501 tttgataata caactatgaa tgaattagca aaagaagtta aatatactaa acgcaccata
    28561 tacaagtact tttcatgtaa agaagattta ttctttgctg ttgtcttaag aggatataaa
    28621 agactttggg ataatgttaa aatagaaagt gcaaaaggca aaacagggtt tgaaaaaata
    28681 aaactttcat actttgcttt tcataaattt tactctgtag aaccttctct attatgcctt
    28741 atgggaatga ttggtattgt aaactctgaa aaatcggata ctgatatgct gtttaaagaa
    28801 aagttttttt cttttaacaa atttatgttt gatgaaatac aaggaatgtt tgaaattgga
    28861 aaaaatgata gaagtattag gcatgatatt gaaataccta ctcttatgta ttcatcaata
    28921 tttacactta caggattttt taatttacta tctgtaacag gaaaatcata cctgaacaac
    28981 tttaatatag atgaggaaaa atttattgaa acaactcttg ctcttttaat agattcaatt
    29041 aaagcataaa cctaagttta taaattaaag tttatatact taaatgataa ttttttctat
    29101 ctctaatata aaaaatatta aacataaaac taataactta gttaatagaa taactaaatt
    29161 actgaaagga ttaaaagtaa atggctaaaa tacttctttt agaagatgat gatacaatta
    29221 catttggaat aaaaacttcc cttgaaaaga aaaatcacac tgtatgttgt tataatacga
    29281 ttgctgaagc taaaaaaaat tttgataaaa ctattgattt aattctgctt gatttaaatc
    29341 tacctgatgg tattggatac gagttttgcc aatatgttaa aagtatagaa gatactccag
    29401 tgatattttt gacagtccgt gatgatgaaa aagacattat acgtggatta gatatgggtg
    29461 cagatgacta tgtggttaaa ccgtttttgc tctcagtact tcaatccaga attaatgcag
    29521 ttcttagaag aactcattca aatactaaca atactattac atgtgaaaat ataagcttga
    29581 ataaggttga agtcaaagca tatttagata atgttgaagt acctttgaca gcaggagaat
    29641 ataaacttct tctaagtctt atggaaaata aaaatagagc tatgactcgt acaaagttac
    29701 tagaaatgct ctgggatgta gatggtaatt tcgtaaatga taacactctt actgttacaa
    29761 taaagcgtct tagagaaaaa ttaaaaaatc caccttgtat aaaaacatta agaggcatag
    29821 gatatagaat ggaggacagc tagtgaaaac tccaagaaat atcaacagta cagttgtttt
    29881 tataatattc tgcttaattg taactttttt attaagtgta attctaacaa ctataacttc
    29941 ttttaatgaa tacaatgtac tgtctaaggc tcttggtgca tcatatgagt acaatcttaa
    30001 gtctacagaa agtatactaa aatcactgaa aaattctagt gataaggatt ttaaaacagg
    30061 aaatgaaatt ttatcaaaac atggatattc taaaagtaca ttttggagta aaaacttttt
    30121 ttattttcta gtgatatcgt ttggtataat ttcaacaatg gctacagttg tatatataat
    30181 caaacataga acaaataagc aaaaactgag tagaataaac aatcttactc aatacttaga
    30241 agaagtgaat ttaggaaaag agtctgtact tttacgatgt gaagatgaat tttctcacct
    30301 tgaagatgag atatataaaa cagttgggga acttcgttgt tcaaaagaat ttgctttaaa
    30361 agaaagacaa actctttcag ataatcttgc agatattgct catcaactta agactccaat
    30421 tacttctatg tcacttatgg cacaattact ttcagaaaat tgtccagatg aagaaataga
    30481 ttatattaac agacttgcaa atcaaatctc aagacttgag agactagttt catctttact
    30541 tacattatca aaattggatg caagtacttt aatatttgaa agcaaaccta tagatgtaca
    30601 ttctcttctt acttatgcaa ttgagcctat agaaggttct ttaagagaaa aacatcagac
    30661 atttactatc gaggcttctc cttcggtaga atttgaaatc gatattaatt ggactttaga
    30721 agctctttta aatattctta aaaattgtag tgagcatata gaagatggtg gatatatagt
    30781 tgcttattat agtcaaaatc ctctatatgt tgagattatc atagaagata atggtaaggg
    30841 gtttgctcag gaagaacttc catatatttt taatagattt tatcgtggta aaaatgctag
    30901 taaggacagt attggtatag ggcttgctct atcaaagtct attatagaac gtcaaaatgg
    30961 tactctacat gcagaaaatt gccataatgg tggtgcaaga ttcataatta aattttacac
    31021 ataaaatgct ggattatagg taaaatacct ataatccagc tatatctata ttctatgtta
    31081 tctattgttt tagtaatata cttctattta catttaaggt tttattaaac aaacaaatta
    31141 actataccta tacttgtaag tagtcttaaa tcgaaacctt taaataagtt caaatagatt
    31201 ttgtttatat tgtttcattt tttatagatt ctactatatt ttcattcttc aactgcttgt
    31261 atcctgtcat atatgatgct attacaataa aaagtatcaa tatagtaaaa cctattattg
    31321 tagatatagg tgcaaatggt aaatactcta taaaatatat ttcatttata cgtagaaaag
    31381 catacactat tcctatttgc acaggaatac taagtagtaa tggtgtaatt cctaagaata
    31441 aaccttccaa aataagcatc ttttttatgc cttctggaga taatccaaca gaacgcaaca
    31501 tagcaaactc ttgtcgtcta cttctaatat ttcctgacac tgtagcaagt acatttgata
    31561 atccaatcat agccaataaa cctgtaataa agcctactat taaattcatg gtctttattc
    31621 cactagcctt ttgtttttca ctttctaaaa catcctcaat actataatct cctgaaccat
    31681 agtctttatt ataaatggac tctaattttt ctcttactct aggaatgtcc tcacgattat
    31741 ttactttaaa tactcctatc agcctatttg aacgtaacct acttacttca tcataatttt
    31801 tagatatttc ctgcaccata gacattggca ctatttgcat taatgtatat tttgaactag
    31861 tatctactat tttaggaagt gtgcttgtaa tagctcccac tttcatgttg taagtataat
    31921 cccctttatc ttcgtcatat actttttcat ttaatttaat gttatctcct attgaaagat
    31981 ttaaataatc tatatatact ttatttctac gtgtactacg attaatatct tcctccttat
    32041 tatatacaat tgaggttgga ttttttgtat cataaaataa ctttgggtct acagaaattt
    32101 gcctacaata ttctgtaaaa ctcttatcat caagcccaat tatatttgaa attatacgaa
    32161 acttgccatc acgttctata ggagagtatt ttttagatga aactatactt ttaaatcctc
    32221 catttttcgc aagttctttt gatgcatctt tttcagtcag ccaagtggaa gcagacattt
    32281 tttgataata tagtgaactt tttacacctt ctgtattatc taatttttca atgaaagcag
    32341 actctacagg ttcactattt tccaaataaa ctgctatatc tctttggtcc ttataattat
    32401 cacttccata aacagctttt gctccttctt gatttgcaat tatatgcaaa aaacatgtaa
    32461 gtaataaaaa agataatgtt aaagatattg ttgcagtacg ataagatttt ttacgagcac
    32521 gtagtgcgtt tgatgcaagt tctccttcta ttccaaatat ctttcctaat gaatatttat
    32581 gcgacttttt gattttaaca ctatctcctt gtcgtattgc ttctatagga gatatctttg
    32641 atacttttcg tgctggtata agtgcagaaa tccagactgt tactattgta agtaaaactg
    32701 ctggcagaaa agcaggaata ccataagtaa ataccacttc tggaacatca gtatgataat
    32761 taactgaatt aatataaact ataagcctat tacacaataa ccatcctaaa aataaaccta
    32821 ttggtaatgg tataattgtc agtaaaaagc cttcaaacac tacagacctt ttaatttgtt
    32881 ttggtgatgc accaacacta gataatatcc ctaactgagt aagtctagca tttgcagaca
    32941 gtgaaaacgc gttgtaaatc atcattacaa acacggctac tgtaaaaatt gctattgtaa
    33001 gatacataat aggagttgaa aattttgata tactactcat agatgctttc tgttctggtg
    33061 aaaagactaa catctttaaa agataattac tattgtattt aagattgtat tttccatatt
    33121 cattagtctt ccatcccaat gatttagcaa gttttggaag ttccttatac gtgtctctta
    33181 tatttttaaa tcgcaagtac acagttatac tatcattagg ttttatactt gttttatcaa
    33241 gatatccaag agctgtatat gcaggtatta ctgatgaagt agaaacatct acctttccta
    33301 ctattgtcaa tgtagcctct gaaatttttt taaaactttc ccctttcgat tttggggcta
    33361 tgggttcaag aggctttcca tttaggattc tatctcctat tgggagagta attttatccc
    33421 caatttttac acttggatta ttctcaaaat attgttttga tagtgcaatt tcattagcat
    33481 tatttggtcg acaaccctct attatagtat ccttttctgg catagaattc caatactctg
    33541 atgtagcatt tctataaatt atataatctc ttttctcatc attaattttg cctacatacc
    33601 attgtccttt taccattaca tcatcaactg aagaaaaatt tccaattaat ggtaagtcac
    33661 ttccataagt atagtcaaac aattcaccat gccagttacc ttctttctca attgaaagtc
    33721 ttataaaatc tgtccacatg gtatacaaca gtccacacaa agaagacatc atggttgtag
    33781 taagtaatat agctaacatt atagctatac tactacgttt attgcgtcgt acaaagtcta
    33841 aggtgtattc ttgaagaata ttcatttctt caccaccttt tcagagacaa tcactccatc
    33901 ttccatagtt acaatacgat ttgcctctag tgctattttc tcatcatgag taataagaag
    33961 tattgtctga ttaaatcgtt tatttgataa gtttaacaag tctacaattt cttctgaatt
    34021 ttttctatca agattaccag ttggctcatc tgctaataaa atagctggtc tatagattaa
    34081 acttctagct atagataccc tttgttgttg tccacctgaa agttgacttg gaagatggtt
    34141 taatctatca gataatccca atatagatac aatctcagag aatctatcct catccacctt
    34201 tcttttatca agtaacatgg gtaataatat attttttcta acatcaaggg taggtataag
    34261 attataaaat tgataaataa gacctacttt tctacgtcta aaaactgcaa gagcttcttc
    34321 ctttaaactt gatatttctg tatcttcaac ataaatcttt ccagatgtag gtctatctac
    34381 tcccccaagc agatgtaaca atgtagattt accagaacca gatgctccta caatggatac
    34441 aaaatcacct ttttgaactg ataaatttac attattaagg gctgtcactc tggtttgatt
    34501 tgaaccatat atttttgtga gattttcaca ttttaatatt tccatataca atcatctcct
    34561 ttatggttaa attttagcaa cctaaaatga cattatagtg acatgaatac aaatataaat
    34621 tactattaaa aatattgttt agtttttgca cattatttaa aattataata taaaacaaag
    34681 actatgcaat atattttatt tatactttta atatattaca tagtctttaa aaggtattaa
    34741 ctttaaatct ataggtattc aatttaataa tcaatagcaa gtattttatg tactaaagga
    34801 gttttgctct atatatactc aatttagtaa aattatttaa taaatattac agactatagg
    34861 taaataatat ttacaaataa aattaagttt attcccttac actataatat ttcttaatta
    34921 tattaattgc aatttccttt acctttggcc ctgtaatttc tttattggaa tcatctgatt
    34981 taatagcaaa ataataggtg tcaccttcac gctctagcat accaacaaac catgcatcca
    35041 cacgtttatt tttttcatca aatcctgttc cagtttttcc atatacattt atgtttttat
    35101 catttacatc tattttcata atatctctta aaatattaat atgctctttt ttaaagtttg
    35161 tatctccctc aaatattttt gccattgttt gcacttgttc ttttggagat atttgtaatg
    35221 aagattctaa ccaaaatcca tttaaatgtc cctttccatt ttttaaatct ccttcccatt
    35281 cgctaatatc gcaattccca tactttaaat catctaaaat gttttggaca gatttagcat
    35341 caactttatc tatcaatttt ttgtagtacc aaacacaaga ctccttaaat gcctcttcta
    35401 aactaagatt tttattccaa tttttatttg gatactctgt accatcatat cccataactg
    35461 attcttttga gtttattacc cctttttcta aaccaattaa agttgaaact attttaaaag
    35521 tagaacatgg tgaccttctt gtttcaatta attctttgtt atatatatta tattctttat
    35581 ttttagtatt ataaaaaata gctcctccac taataccttc aaaacaatca ctatagtcaa
    35641 ccatattaat tttttctttt ttgtttttat cattgttttc aactttggat ttatttaatt
    35701 cagagtttat atgattctta tcatgttttt gttttgtgtt gttatgcttt cttcctacat
    35761 aatatactcc catagctaca acaactagaa gaataactat ccaaataaaa ttattttttc
    35821 ttttcatact ttataccatc tcctctacat aagtttatag ctcaccttta aaataatata
    35881 cattatttgt aatatactcc aatctaggag cttgaatact tctatttata aaatctaact
    35941 ttggtttaac atgttttaaa tctttaaaaa tcacaacacc atcaatagat tgtaattcag
    36001 ctccaaagaa aaaatagact aaatttctat tcccagaaat tttaactaca tctaaatttc
    36061 tgtttagttt tttcattttt tttattactt ctttagaaaa tatcttctcc aattcatttt
    36121 tatcagattg aatataaaag acagattcag agcttaactt tttttcatca taaactttca
    36181 aaatagcttt agctactaca ctataatctt tttcataaag tttaaatcta aaactttcac
    36241 ttggactagg aataaatata gtacctatac ttataaagat actaaatata catccaatca
    36301 tttttctttt atttaaatct tttttatagt aaagcttaat catatgtaca caaatccata
    36361 taaatgcaat tagcatcaat ggtattgcaa ataaacctat aaatagtagc attactcctc
    36421 ccctacatat tatatttcca cacaaaaata aaaaatcact tacaataaat agagttaaag
    36481 ggataactat atctcttata ttttctttac ctaatataat tatgttgatt gaggctatga
    36541 tgaatatact caatataact attagtatca ttatgtcctc ctttcagttt gtgaggtaat
    36601 tatttattct ttatttaaaa cttactattg taatatttaa atattacaat agtaagtttt
    36661 aaatgtcaag tcgttttaat tgtataatta tctttgtact tatcaaatta cgagtatcta
    36721 ttatatactt ttttatctat tatcaactat catttattca gattgtatgg gattggttaa
    36781 atttagaaat accttctata acatagcaga atatatcaag aaagtagatt ttaattttat
    36841 tataatatgg ttacataata taattgcttt actaaaatgt actcagaaag gatttaacat
    36901 tcatgtcaaa tattataata aaaggactta cacaaaataa cctaaaaaat atatcattta
    36961 aaattcctaa aaataaaatt gttgttttta ctggagtttc tggttctgga aaatccagta
    37021 tcgtatttga tataattgca gcagaatcac aaaggcagat gaatgaaaca tattcttcat
    37081 ttatcagaaa taggctacct aaatatagaa aacctcaggt ggagctgatt gataatttaa
    37141 ctgcttctgt aatcgtagac caaactcctc ttggtagcaa tgcacgttct acagttggca
    37201 ctataagtga tttgtattca tcattaagat tattattttc tagaattgga gaaccctaca
    37261 taggaacaac ttcatatttt tcttttaatg acccaaatgg tatgtgtaaa aattgttctg
    37321 ggattggtag agttaaagta atcaatattg aatctattat aaatcctgaa aattcatgga
    37381 atacaggatg tataaatgac tctttattta aacctaattc atggtactgg aagcagtatg
    37441 cagagtctgg actttttgat ttagataaac ctataaaaga ttattctttt gaagaatata
    37501 atttattgct ttatggttca aaaaataaag atggaaaaca agaaaatcca aaaatagaag
    37561 gtatttttaa caaatacaat caaacatatc taaatcgaga tattagcaat atgagcaaac
    37621 acacaaaaga aaaatcagaa agacttataa cagataagga atgtcctgta tgtcatggaa
    37681 aaagattaaa taaagaaact cttaactgta atattttagg atattcgatt gcagatatgt
    37741 gcaatatgga acttactgac ctttatgatt tattgttaca aataaaatat aaaagaactg
    37801 aaactgtaat aaagactctt actgatggtt taaaaagaat gattgatatt ggtctccctt
    37861 acctacatct aaatagagaa tcatcatctc tatctggagg agaagctcaa agattaaaac
    37921 ttgtacgcta tatggggagc agtctcacag gtatgactta tattttcgat gaacctagtg
    37981 ctggtatgca tccacgagat gtacatcgta tgaatcagct attaaaaaaa cttagagata
    38041 gaggaaatac tgtccttgta gtagagcatg ataaagatgt tatctccata gcagatgaag
    38101 ttatagatat tggtccaagt gctggtaccc agggtggaca tgttgtattc caaggctctt
    38161 atgataaatt acttttagca aacactctta cttcaaatgc aatgaatcta tcattaccta
    38221 taaaaactga ctctcgtcat ccaaaaggta cccttccaat tagagatgct tgcataaata
    38281 atctaaaaca tattgatgta gatttaccag ttggaattat gactgtcgtt actggagtag
    38341 caggttctgg caagagtact ttaatctctc gtgtttttgc acaaaaatac gaaaaagatg
    38401 ttattaaaat cgaccaaagc ccagttacag ctacaaatcg ttctatgcct gctacatttc
    38461 ttggtttctt tgatgagata agaaaagtat ttgctaaaga aaataatgtt gaagaaggtt
    38521 tattcagctt taattcaaag ggagcttgtc ctgtttgtga tggtaaagga gtacttgtta
    38581 cagagcttgt attcatggac cctattatta atacatgtga agcttgcaaa ggtactagat
    38641 atagtgaaga agttttgtca aaattatata aaggtaaatg tatagttgat gtactatcta
    38701 tgacagtaga tgaatctctt gaatttttta cagaaaagaa gctatgtaaa atcttacaaa
    38761 caatgaaaga tgttggttta ccttatatga ccttaggtca accacttagc acactatctg
    38821 gaggagaacg tcaacgaatt aagttagcta aaaacctcaa caaaaaaggt actatatata
    38881 ttttagatga accgactaca gggcttcatg gttctgatat tgaaaagctt atgttgattt
    38941 ttgagcatat agttgataaa ggtaatactg taattataat tgaacataat ttggatgtat
    39001 tgaaacaagc agactgggta attgatattg gtcctgatgg tggcaaaaat ggtggtcagt
    39061 tcgtatttga aggtacacca ctagaaatgt ttgaaagagg ttgcactata acatcagatt
    39121 tccttagaaa atcaataaat atatagataa tataatacta atattaagaa attaagaact
    39181 attacatata tattactact ttgttttatt tcataaattt aaattttaac tttattttaa
    39241 aagaaattaa cattataaaa gctataaatc acaaaatttt ataaaatttc aaatgtaatt
    39301 tatagctttc attttttata aatttataag tattattttt tgtcgattta ttgatgaatc
    39361 tttgtcccag tttatattta cgataataaa tgcttataag atataatcta catgcttata
    39421 agttgagtta atgtaccaac atatgttaac ttaagtttgt gcatagctcg tgtacaagca
    39481 atatataaca aactgcggtc ataatcacta taatatgttt tattatttac tgaaggtaca
    39541 ataacttcat caaattctaa accttttgac attttaatag aagttatgga tactcctttt
    39601 gtaaagctag aactttcaga agatattaag tgaacactat attcttctct taacgcatca
    39661 tatacagctt tagcgtcatt atctgtcttt aatataatac caagagtcgc attatcactc
    39721 tttttaaatt cttcaatttc tatttttatc ttatttattt catcttgctc gttattacat
    39781 tttaataaag ctggttcttc accatgtcgt tcaataggct ctaatgaaga tatgtcttga
    39841 atctttttgg cgaaattaat aatctcaaaa gtagaacggt aactcttgtt caacataact
    39901 aattccccat cattatatag ctgtttcata tcatctaatg tatgagtatg attaggatta
    39961 accaattgtc caaaatctcc aagaatagtc tttttacact taaaaagtaa gtttattaca
    40021 gcatattgaa taggtgtata atcctgcatt tcatcaataa caatatgcct tataatctta
    40081 tcttcttgta taccttcata tgcagcatga atatatataa atgggtatac atctgaccat
    40141 tcaagtgttt tcttttctac catcacaaac atgtttgata tattcatttg tttaaagaaa
    40201 tccttatata gtgtaagagt attttttatt gtcaacattc cattcaaact tttaagtata
    40261 ctttttacct ttggtaaatc ttcttccata atattatctg attcaaattt ataatgaatg
    40321 tcttcagcta ccttctctaa tctcttctta actggatatc tgttatatgc ctcaaatcta
    40381 ctctgtatcc agtcactttt agctgtaaaa cttccaaaag tgtagtcttt tggaataaaa
    40441 atcttatttg gcatttgttt tatataatca tcaataagtt ttacaaaatc aagtgtagat
    40501 ttaaaacgca ctctttcaga ccatttagca tcatttattt ctaatgggtc tttctcactt
    40561 tcaaaattaa taactctatc taattgtacc tctgctatat tttcaaaact taattcacat
    40621 aatggctctt ctccaagctc aggaaggaca ttggaaatat agtctccaaa gactttatta
    40681 ggtgatagga taattacatt atttgctgat attttatctt taaatcgata aagaagaaaa
    40741 gcaattctgt gtaatgctat agatgttttc ccagaaccag ctaccccttg aataatcagt
    40801 gtatcagctt tatcatttct aataatttga ttttgttcct tttgtattgt tgaaatgatt
    40861 gacttcattt tttcatctga agtgtgacta agttcttttt gaagaatatc atcttgtata
    40921 ttaatggaac tttctagtgc atattctaat tttccatttt taattttaaa ttgtcgtttt
    40981 cttgttattt ctccatccac ccatcctatt ggagcatcat atccagcttt tccgatttca
    41041 taatcataaa acatactggc aattggagaa cgccagtcaa gtattataag ctcatcttca
    41101 taatcaaatg caaatctacc aatataatac ttagattcat catccatatc tttcagccta
    41161 aaatctattc tagcaaagta tggagaatct ttgattttat taatcttatc tcgaactttt
    41221 accataaaag ccccatagtt atctatttgt tttagtcctt gttcattttg aaacatttca
    41281 tgtgggtcta tttcatcacg attttttacc atatacattt tagcgtccat ataatcctta
    41341 tcaagcttgt caacaagatt ttcagctttc ttaaaagcat tgtctagctt gttatttatc
    41401 tctattagat gtgcaatctc atctggaaaa aaagttaata actctgcatc atattcacac
    41461 atattatgtt tagaattaga tgcaataata tcgcaattta ctacttgttt attttcagac
    41521 atttaaatcc tcctttgtta attaagtact tacactgaat cattatactt aattgtgttt
    41581 tggagttttt aatcattctt gctatttaac ttaaatgtta gctttttagc tttaaagttc
    41641 cttttttaaa aaactaaatc tttctatatt tattaccata agtacactta caagcaaaat
    41701 acattaaaag cagaaatgct cttatatata aaataattca aacaaaaaat atgtggtaaa
    41761 cttttcattt aatctacaat taattttgtc tattatctct ttcttctaca caaggaagaa
    41821 taaatttttg agtaggatat ccaaattcca caagcaattc tgcttctgca aagcccaact
    41881 tctttatcat atctctatgt cctgtatcag ctttgtcacc ctctcgaaag gtagtaattg
    41941 aaatatctgt atctatcaag aaatcttcaa taactttctt caaaaacact tgtgcaatgt
    42001 ctgaattacg atactgtgga tgtattccaa aaaaatcaat acttctagtt tctctattaa
    42061 acaccatatt tgcaatagcg acattattaa gttttaatat taaagctctt tcttctaaaa
    42121 tatactgttc taattgatat atatattctt cctcttgcaa atttggaaat ccatctatga
    42181 ctaatctcac taaattcatc cataatggta tgtcagacat acttgcaagt ttaatttctt
    42241 tttccaactc tagtacattc tttgaaaata ggttatcttt tttcaaaaca aatcttagct
    42301 gcaaaggata aaattcttgt tctttcctat atttatttgg agatttttta tacatagctt
    42361 taaatataga agtgaatgcc tgttgacttt catatccagc tatcaatgca atttcaataa
    42421 ttggtttctt tgaaaatacc aataattttg ctgcttctgt caactttctc cgctttatgt
    42481 aatcatgcat tgttaggcca acagaagttg tgaaagttct atgcaggtga tattttgagt
    42541 aatgaacagc attagcaact atatctaaat ccagcttttc tgataaatgc tcctctatat
    42601 agtcaatagc agttatgaca ttaattactt tgtaagacat tgcagcttcc ctccttttat
    42661 ccatgcaaca ttatatcaat tattacttag ttatatttaa tgaatcttgc tattttataa
    42721 acaaatttgt tgtttaagtt ttcaattcat aaatttaata tattactttt tactttctga
    42781 ttaaagactc taatccttt
//
LOCUS       DAEMHW010000010        42171 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEMHW010000010 DAEMHW010000000
VERSION     DAEMHW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42171
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.010"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..637)
                     /locus_tag="KPK67_000428"
     CDS             complement(<1..637)
                     /locus_tag="KPK67_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HBG2457437.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     GYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMF"
     gene            complement(667..1776)
                     /gene="ugpC"
                     /locus_tag="KPK67_000429"
     CDS             complement(667..1776)
                     /gene="ugpC"
                     /locus_tag="KPK67_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBG2457438.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIYVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            2035..2772
                     /gene="cas6"
                     /locus_tag="KPK67_000430"
     CDS             2035..2772
                     /gene="cas6"
                     /locus_tag="KPK67_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG2457439.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            2777..4252
                     /locus_tag="KPK67_000431"
     CDS             2777..4252
                     /locus_tag="KPK67_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457440.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELIKKIELIDIIDNYNFSREA
                     ANTKNLGKKMIDNGNLSNLKELLLDNISVLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            4272..5285
                     /locus_tag="KPK67_000432"
                     /pseudo
     CDS             4272..5285
                     /locus_tag="KPK67_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
     gene            5301..6107
                     /gene="cas5"
                     /locus_tag="KPK67_000433"
     CDS             5301..6107
                     /gene="cas5"
                     /locus_tag="KPK67_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBG2457441.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCNVCIDTKEVFTWL"
     gene            6134..8542
                     /locus_tag="KPK67_000434"
     CDS             6134..8542
                     /locus_tag="KPK67_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG2457442.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYVNNILNVDFKFGEYVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIVFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEGALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTKSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            8983..9930
                     /gene="prmA"
                     /locus_tag="KPK67_000435"
     CDS             8983..9930
                     /gene="prmA"
                     /locus_tag="KPK67_000435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBG2457443.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            9949..10707
                     /locus_tag="KPK67_000436"
     CDS             9949..10707
                     /locus_tag="KPK67_000436"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBG2457444.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            10709..12007
                     /gene="mtaB"
                     /locus_tag="KPK67_000437"
     CDS             10709..12007
                     /gene="mtaB"
                     /locus_tag="KPK67_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBG2457445.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKNINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMSDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            12050..12400
                     /locus_tag="KPK67_000438"
     CDS             12050..12400
                     /locus_tag="KPK67_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBG2457446.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            12563..12742
                     /locus_tag="KPK67_000439"
     CDS             12563..12742
                     /locus_tag="KPK67_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBG2457447.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            12772..13215
                     /locus_tag="KPK67_000440"
     CDS             12772..13215
                     /locus_tag="KPK67_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBG2457448.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            13326..13823
                     /locus_tag="KPK67_000441"
     CDS             13326..13823
                     /locus_tag="KPK67_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBG2457449.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            14016..14579
                     /gene="raiA"
                     /locus_tag="KPK67_000442"
     CDS             14016..14579
                     /gene="raiA"
                     /locus_tag="KPK67_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG2457450.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            14792..15028
                     /locus_tag="KPK67_000443"
     CDS             14792..15028
                     /locus_tag="KPK67_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBG2457451.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            15041..16384
                     /gene="yqfD"
                     /locus_tag="KPK67_000444"
     CDS             15041..16384
                     /gene="yqfD"
                     /locus_tag="KPK67_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBG2457452.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            16323..17438
                     /locus_tag="KPK67_000445"
     CDS             16323..17438
                     /locus_tag="KPK67_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBG2457453.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            17495..17956
                     /gene="ybeY"
                     /locus_tag="KPK67_000446"
     CDS             17495..17956
                     /gene="ybeY"
                     /locus_tag="KPK67_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBG2457454.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            17962..18681
                     /locus_tag="KPK67_000447"
     CDS             17962..18681
                     /locus_tag="KPK67_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBG2457455.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            18800..19201
                     /locus_tag="KPK67_000448"
     CDS             18800..19201
                     /locus_tag="KPK67_000448"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG2457456.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            19265..20158
                     /gene="era"
                     /locus_tag="KPK67_000449"
     CDS             19265..20158
                     /gene="era"
                     /locus_tag="KPK67_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBG2457457.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            20170..21549
                     /gene="mgtE"
                     /locus_tag="KPK67_000450"
     CDS             20170..21549
                     /gene="mgtE"
                     /locus_tag="KPK67_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBG2457458.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            21622..21777
                     /locus_tag="KPK67_000451"
     CDS             21622..21777
                     /locus_tag="KPK67_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBG2457459.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            21743..22516
                     /gene="recO"
                     /locus_tag="KPK67_000452"
     CDS             21743..22516
                     /gene="recO"
                     /locus_tag="KPK67_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBG2457460.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            22509..23108
                     /locus_tag="KPK67_000453"
     CDS             22509..23108
                     /locus_tag="KPK67_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBG2457461.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            23268..24146
                     /gene="glyQ"
                     /locus_tag="KPK67_000454"
     CDS             23268..24146
                     /gene="glyQ"
                     /locus_tag="KPK67_000454"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBG2457462.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            24146..26212
                     /locus_tag="KPK67_000455"
     CDS             24146..26212
                     /locus_tag="KPK67_000455"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBG2457463.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVTLLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKILIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRAVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(26378..27925)
                     /locus_tag="KPK67_000456"
     CDS             complement(26378..27925)
                     /locus_tag="KPK67_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBG2457464.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNLNDNILNK
                     ITDKYKI"
     gene            28205..28972
                     /locus_tag="KPK67_000457"
     CDS             28205..28972
                     /locus_tag="KPK67_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG2457465.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            29255..29452
                     /locus_tag="KPK67_000458"
     CDS             29255..29452
                     /locus_tag="KPK67_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2457466.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            29481..30557
                     /gene="vorB"
                     /locus_tag="KPK67_000459"
     CDS             29481..30557
                     /gene="vorB"
                     /locus_tag="KPK67_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG2457467.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            30560..31279
                     /locus_tag="KPK67_000460"
     CDS             30560..31279
                     /locus_tag="KPK67_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG2457468.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELIPKYPLGEFID"
     gene            31292..31831
                     /locus_tag="KPK67_000461"
     CDS             31292..31831
                     /locus_tag="KPK67_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG2457469.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNIEAFEAGYDYE"
     gene            31988..33046
                     /gene="buk"
                     /locus_tag="KPK67_000462"
     CDS             31988..33046
                     /gene="buk"
                     /locus_tag="KPK67_000462"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG2457470.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            33064..33957
                     /locus_tag="KPK67_000463"
     CDS             33064..33957
                     /locus_tag="KPK67_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG2457471.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            33987..35195
                     /locus_tag="KPK67_000464"
     CDS             33987..35195
                     /locus_tag="KPK67_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2457472.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            35219..36478
                     /locus_tag="KPK67_000465"
     CDS             35219..36478
                     /locus_tag="KPK67_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2457473.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYSNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
     gene            37123..38586
                     /locus_tag="KPK67_000466"
     CDS             37123..38586
                     /locus_tag="KPK67_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG2457474.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALSGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            38642..39778
                     /locus_tag="KPK67_000467"
     CDS             38642..39778
                     /locus_tag="KPK67_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2457475.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            40014..41129
                     /locus_tag="KPK67_000468"
     CDS             40014..41129
                     /locus_tag="KPK67_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBG2457476.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEILEGQINKSNVVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            41281..42150
                     /locus_tag="KPK67_000469"
     CDS             41281..42150
                     /locus_tag="KPK67_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2457477.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPAIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKIHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGNLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPN
                     NVNSQIDIYIPIK"
BASE COUNT        16800 a         4356 c         7670 g        13345 t
ORIGIN      
        1 aaaacatact agtagatgtc ttagaaatat ttaaatcatt atttataaat tttattcctg
       61 tatatagaag ttcttcatca atagatttaa tcttattaaa atctattaat ttattgagta
      121 tattatcctc attaattaaa tctaaagttt tatatccaat aaggtcaaac aaatttaaaa
      181 acttattttc agcgttgtct tttataatat gaggtagtaa atcagaacat aactgtaatc
      241 taaaatcaaa atcagcttta ctatctaatt caccaagata aaaaatataa gtactttgat
      301 aagtttcagt ttcaattata ttttgtgtag ttacattaat atctaagtta tatttagaat
      361 ttactataaa aaaaccattg tgttgtatta tatttatatc ttcaaataaa ttataaaaaa
      421 tattttttag atattcaaag tctatatgag attggtaaat taaataaaag ttgcctttta
      481 aattatcgag attattttta tctagaaaat caataacata aatagcatta ttgaaagtaa
      541 ctaaatggct actagcatct aacttttggt tttcatatgg atagatattt atttttttat
      601 taagctgtct ttctaaaaaa tcttttaact cttccataaa gtctccttaa aaataagatt
      661 atattcttat cttatagcta aagtagtttc tttgtcaaat atgtgtactc tactattgtc
      721 aactgaaagt gttaagtcat caccaacatt aattctgttt ttactgtcaa atctaattgt
      781 tatcgtattt tcgcctagtt ttaagtgagc ataagtttct gctcccataa gttcttttat
      841 ttcaattctt gatttaaaac aagtatctgg attagattta ataaaagtat cttctacatg
      901 aatatcttct ggtctgatac ctataacaac ttcattatta atataattgt cttttattaa
      961 atcatagtgt ttttctttat tcaatttaag cttaatatgt tcattttgtg cataaatttc
     1021 atcattttct tctattaata ttacataaat aaaattcatc tgtggtgctc caataaatcc
     1081 tgcaacaaat atattagctg gataatcgta tacatcttgt ggagttgcaa tctgctgaac
     1141 tactccatcc ttcattacta cgattctatc agccatagtc atagcttcaa cttggtcgtg
     1201 tgttacatag ataaatgtag ttccaagctg ttggtgtagc ttagttatct ctgttctcat
     1261 agcagttcta agttttgcat ctaagttgga tagaggttca tccattaaga ataccttagg
     1321 atttctaact attgcacgtc ctaaggcaac cctttgtctt tgtcctccag ataatgtttt
     1381 aggtttttta tttaaaagag gtagtaagtc taatatttta gcagcttctt taactttttt
     1441 gtctatttca tctttaggca attttctaag tttaagtgca aatgccatgt tttcgtaaac
     1501 agataagtgg ggatataaag catagttttg aaaaaccata gctatatctc tatcttttgg
     1561 ttctatatca ttgacaagtt tatctccaat gtatagttct ccttcagaga tttcttcaag
     1621 accagcaatc attctaagtg tagtcgattt accacaacca gatggtccta ctaaaacgat
     1681 aaattcttta tctttaatgt ctatatttat attttttact gcgttaaatc catttgagta
     1741 aagttttgat atatttttta aaattacttc tgacataata tcctccttga tatttaaatt
     1801 ttaattatat agtatcaaat ttttttatta tattaaatgt gtaaaatgac taaatgtatt
     1861 tgtttaatat gtatagttag tttttagcaa agaataaaac taacagtaaa atatatttaa
     1921 aaaattaaaa aacaaaatta aatttattat caaaaaatca ttttatgata tgttttattt
     1981 tttatattac aaatagagaa taatgaatta tactagaaag taggaggtga aaatatgagc
     2041 agaatttcaa tttttatgga gtcatgtaat ggcaaatttc caaatgaaaa ttcaatgttg
     2101 tcagtttcct ttataaaaaa tattttaaat gcagaaaaca aagattttgc taaaaacata
     2161 tttaactatg gggaagaaag aaaaaacaat aagcaaataa aggatataaa tacagcaatc
     2221 tacataccaa atcttataag aagcaaaaat gagttacttg ttgatggaga tataatattc
     2281 aatataagtt tttataatta ttctatgttc agtaaattat acaatggaat tttaaaagta
     2341 aaggagctag aatataaagg ttatagattt aaaataaaaa atataaaaat acataaagaa
     2401 cacgaaataa aagaaaatgg tgttatattt aaaactatgt ctcctataat agtaaagaat
     2461 aaagaaggta aatatttaga tgtagaagat tcaaattata tagaatgttt aaactatata
     2521 gctaatctaa ctctggagag tataagaggt agtgggctta gaaagccatt ggaatttata
     2581 cctcttaact tcaaaaaaag agtgttaaaa gaaaagattc gtggatttaa agtgagagaa
     2641 tattactata taaatgctta tgcaggaaca ttttttttaa aaggaaatat ggaagattta
     2701 aatgcactgt ataaaatggg aattggatat agaaggacgg aaaatgcagg aatggttgat
     2761 atattaaagt agcgaggtga gagaaataaa agctaaattt tatagatcag gtgatattgt
     2821 aagagatatt ggcattgttt acttttatga attactaatg gatttaaaaa acgaactaga
     2881 aaaaaatgat tataagctaa aatttacttg tgagttaaat agaaattatt tatctttaga
     2941 atctgcagaa tatatagacc ctaagtatat atctgattat atattagaaa atcaactttt
     3001 taaggtattt aaaaatggta aaaaggaaac cttagaaaaa atatttagta atatagataa
     3061 tttaacgtat gtaaatttta ttgaagaatt agataaatct tctgcaactg agaaacaaaa
     3121 ggaagggatt aaaaaagatt ttaaaaacag aagatttcca tatgttagaa atagtggtaa
     3181 gtatggatta aacacgagtt tagaaaatat ggaaacaaat gtaaaaagaa tagtagatac
     3241 tgtaattaaa tcaaatatag agatagatga aatagataaa cttgatttga cacaatatga
     3301 agaaaaagat agtgtatgta atgtttgtaa tataaataaa acaacaaaac tagatattga
     3361 tttaagagag cgaaaggact ctaagtataa ctttttattt agaggtgcag aaaaatcagg
     3421 ctttaaaaga agtggacaag ttgaaagtaa tatttgtttt gagtgtgaat ttttcaactt
     3481 aatgtgtctt ttatatataa atttaaaaag accaatggtt tttgcatata cgaatgattt
     3541 aagggaatta gcatttttaa atcataagat aatgcttaaa agaaagatgt attcagataa
     3601 atcattttat aaaaaacttt tgcatgaaaa aataagttca attagactat ataggtttga
     3661 tattgatacg aataaaggta taattctaaa atttgactct attatagagt ataaagaatt
     3721 aataaaaaaa attgaactta tagatattat agataattat aatttttcaa gagaagcagc
     3781 aaatactaaa aatttgggta aaaaaatgat tgataatggg aatttatcaa atttaaaaga
     3841 attattactt gataatataa gtgttttaaa gcaatcaact ggaacttctc gagaaatgga
     3901 ttttgtatct agctcatata atatcggttt atatataaaa ttgtgttgga ttgtagatgg
     3961 aggggaggag tataaaatga aaaactatat ggaaagtaat ttaatatatt caagagtagg
     4021 taaagactta tttaataaaa tgacagatga atccagaaga aaaaattttt ctatgaaagt
     4081 gattcaacta cttaagtcta atgatagaac tcagctattt caaactataa tgcatgtgtt
     4141 agtttctaat ggaatcttaa taggagaagg gtttgtagaa ggtctaatgc agagttctga
     4201 attagaatta aatacgaatg taggtctatt tattcaagaa atcatgaaat aaaaaagggg
     4261 gaaaatttaa aatgaatgaa aaaagagtaa aaggagtaac attaactata ctgacagaaa
     4321 gcccagttcc actttcatat gaccaagggt atggaaatta gacaccaata aaaaaagaac
     4381 aatatagaga aaaaatacat gcaaaaacta gtatagccac aataacatat gatttaagaa
     4441 gaatgctcca ccaagaatat ggttggaatt taagtaatat tatttttggg aaaaaaggaa
     4501 atatatatcc atctattaag caaaatgtag gatgtgtaga tgaaaatgga ttagagactg
     4561 atgtatttgg ttatttgata ccacttaaag ataaaggttc tataagtaag gcttctccgc
     4621 ttagaataat accttttagg tcattaaatt catatagagg ttcaacacag ttgataacaa
     4681 atagaggttt tttaagtagc gaatttggta gaaaatatta tgatgagaag gaagaaaatg
     4741 aagtacttag agatgaaaac tttccaacat cacaagcctt ggcaatagaa gagactttat
     4801 cagattacta cgtttataca ataacattgg agcttgatag aataggagta gtagaagtag
     4861 aagatggaaa gttgctttta ccagaagaaa gaaaatttat gagtaaggag ttaagagaaa
     4921 aagcagtaaa agatatatta gatgcaatta ctgtgtttac aagaaacata aaacattcat
     4981 cagtactgtt aaaaccactt gcagtaatgg gtggagcttt tgataaagtt gtaccgtttt
     5041 tttgggatga tgtagattat aatgctgata gtggtgagat aaatttagag ggagtcatag
     5101 aaactataga aagttatagc cttaaagaaa gtaacacaat acttgctatc aatgacagat
     5161 taaaaatttc taataaaaag gaattaaata agtttaattt aagtaaatac cctgtaaaag
     5221 agattaaaaa tctagcagat agattggaaa ttggagaaga taatatgtgg tatttaaaag
     5281 agtaggaggt tttttgaatt atgaaagtat tacaatgtaa acttaagcaa ccaactgctc
     5341 actatagaga cccaaaagta tttcaaaatg aatatataag tactttaaat ttaccatcta
     5401 agaccacaat aatgggaatg ataacgtatt tatgtgatag aagattaaac tctgatatag
     5461 atataggaat aattggaaca catcatcata gagaactaga attttcaaga ggagagaaca
     5521 ttgatttttg gaatgaatat acaaatatga gaaaaggcaa agataaagaa aagtttttgc
     5581 ttcaaggaaa ctactatgat tactataaag aacataaagc tcaaaatagc atattaaact
     5641 atgaagttct taaagaagta gaactgacta tatttatttc ttgcaaggat gatgaagaac
     5701 ttgaatttat tagaaaaaag cttgaaagtc catgtaaata tgcaaattta ggaagaaaag
     5761 aagattttgt aataccaagt gaaaaagggt gttttgtgaa agaagttgag ttaaaagagg
     5821 ttatacctat gaatactcgt gatgctataa aagaaaatat aaaattaaag aatacatatg
     5881 taagagttga cttaagagat aaagagaatg tcgagactat aataaatcaa ggtgtattga
     5941 ttgcattacc tcataaatat aaagatttag aagcaaatag agatgataga gtttatgagt
     6001 tttgtcatta tatatatgtt gataatgatg gtatatatcc taaaaatatt aaggtaaatg
     6061 tatgtaatgt atgtattgat acaaaagaag tttttacatg gctttaaatt attttgaaag
     6121 ataggtgttt atcatgaaaa atatcatgca aaaagaaata ttagctaagt catataattt
     6181 aagaactaat caacaagagc aaacattagt tgaacatatt aaggatttat ttgaagtatt
     6241 agaatctata ttagaactaa atctatattc agataaagat gtagagatac taaaaatatg
     6301 ttgtgctttg cacgatttag gaaaaattaa ttcaattatg caacaaaaaa tggagattaa
     6361 taataaaata tcgtatagtt gctcagaaga agagaggaaa aagttagagt ctgacaaaaa
     6421 atctttaaaa aagatagcaa gacataatat attttctgga gcatttttaa aagatatatt
     6481 agaaaaaatg aacttgtcag aagaagataa gttatatata tataaaagta taatgttaca
     6541 tcatggaaac tatgaagatt atatgaggtt aagtattagt aaagttcaag aagaaattta
     6601 tgagtatatt gaaaaaggca ttttagaaaa agaagagttt aatctgaaag atatagaaag
     6661 ttatgtaaat aacattttaa atgtagactt taaatttggt gaatatgtat tggattatga
     6721 ttacatagat aaattaagtg aaagtatgtt tatagacagt gattataaca atggtcaaat
     6781 agatgattca gttttaaatt atagaaagtt taagtatatt ttatataaag ggatgctgaa
     6841 cttaatagac catagtgcat caagtagaca gaaaggtata aaattttata acgattttac
     6901 tgatgaagaa attgataata tgattttaaa tgagatatat aagagtcagg gtaatttaga
     6961 aaaaaataat atagagttca atactataca aaaaaggttg agagaacttt ctggaagaaa
     7021 tgttctgact gttgcattta caggttcagg aaaaacagct gcagattata gaaagacttt
     7081 taaaagaaaa ttctttttag tgccaaataa aatatcagca gaaagttttt atagaaaaaa
     7141 tatttttcaa aataaaaatg atttagatac aagaagtagt aatgattaca ttggattact
     7201 tcatggagat ataaacttat attcagagaa tgaagataat ggagaacatg attttgtatt
     7261 gacattaagg gatatagatt tgagtataaa cttttgcaaa ccttgtgtaa ttgcaacagt
     7321 tgaccagtta ttacttagta tgtttaaatt tccgggatat gaaaaaatat tcgcagccgt
     7381 aaaaaatgca tctataactg ttgatgaagt acatttattg gaaccaaaga tgtttttgat
     7441 tttgatatat tttatgcagt ttgcatgcaa gtacttagat gttgattttc acttaatgac
     7501 agctacactt ccaaaatcat ataaagagca aatgataaat aagggaattg tttttcaaga
     7561 agaaagtaat gaaaatgtta ctgataaagg agaaatagtt tttattgaga gtaacaagga
     7621 agaagaaatt tgtgaaggta aagatgtaaa ggtttctttt ataaaggaaa aagaaatcaa
     7681 gtcaatagtt gaaggtgctt tagaaaataa acaaaaaatt cttataataa agaatactat
     7741 tgattcagtc aatagaacat atgagttttt aaaagaaaat ttatctgata agtatggtgg
     7801 tgtagatata gatgttttac atagtagatt taagttcaaa gataaaaaag agaaatatag
     7861 taaaatatta aatggagagg gtgatatttg gatatcaact caatcagtag aaatatcctt
     7921 ggatttggat ttcaatataa taataagtga tttagcaact atggatagct taatacaaag
     7981 aatgggtaga tgcaatagaa ataataaata tgaatatgga aacttttata ttctacctag
     8041 tgaagataaa atttatgata acaaacttaa gaacacaact aaaaacatct taaaggacat
     8101 attaaaaact aaatctattt ttacaatggg aattagaaaa actattttgg atgattacta
     8161 tgataatagt gtagttaaaa aatattttga agagaatttt ataagttgtg ataaagaaat
     8221 taagaatatt tatggtataa ataaagaaat ttttgatgga ttggatttaa tttttaattt
     8281 tgaaccttac aaaaatattg ttgatagtaa gaaggaagca gctaaaattt ttagagatgt
     8341 tgatgttagt tacaaaatta ttctggaaga agatttttat aaggaagata gggagttaca
     8401 acaagattcg attcaagtat cagggtttat atttaataga ttatattatt atggattaat
     8461 aagcaaagta gaaggataca tggtactaaa atctagtagt aagtttgagt ataatagtac
     8521 tgtaggctta attttgaagt gagcatatgt ttaaatataa tataccttta gacattgaaa
     8581 atacaaaaag tactactgta agtccactgt aaaaactatt ttttaaaagg aaaaataagt
     8641 tgtgagatag agtgtttaca agtgtatata gataataaat ttgaggttta agattaacta
     8701 agtgggtttc aggtacatag caaaaataat tataagttgg agattttgta tgtttaagat
     8761 taactaagtg gatttcaaat attgatatag tagatataat agaagtttta ttattaggtt
     8821 taaaattaac taatcggatt tttaaatatt ctgactatta tataaggata ttattagctt
     8881 gaatttaaaa ccaataatat aaaacaacaa aatattatat ttatgatata ataaaagtta
     8941 tggaaaaaca ggaatattac tatagagaag agaggtaaac ttatgaataa ttggatagaa
     9001 gtaactataa aaacaacaac agaagcagtt gaacctataa cgaatatact atatgaacaa
     9061 ggagctggag gagctgtaat agaagaccca aaagattttt tattccaaaa gaaaaatgaa
     9121 ctagactggg attatgttga agaagaagta tttaaaaaga atgaagaaga tgatgttctt
     9181 ataaaaactt atgtttctga agaaaaaaat gtgatggaat ttgtcgaaat aataaagcaa
     9241 aaagttttag gattaaaaga ttttggtata gatataggtg aaggaagtgt atcattggac
     9301 caagtaaatg aagctgattg ggcaaatgca tggaaagcat actacaaacc aacaaaagta
     9361 ggacaaaggg tagtagtaaa gcctacatgg gaagattatg ctatgcaaga gggggattta
     9421 atcatagagc ttgacccagg aatggcattt ggtacaggga cacatgagac tactagtatg
     9481 tgcattagag aattagaaaa gtatgtgaat aaagattcta aagtatttga tattggatgt
     9541 ggtagtggaa tccttgcaat agcagcagct aaattaggag caaaagaagt tgtagctgta
     9601 gatttagatg aagtggctgt aaaagttgca aaagaaaatg ttcttgaaaa taaagttgaa
     9661 aaaagtgtat ctgtaatgca tggtaattta actgatgtaa taaaagataa agcggatgta
     9721 atagttgcaa atataatagc tgatataata aagatacttg caaaagatgt tcaaaacttt
     9781 atgaaagaag atgctatatt catatcttca ggtataattt tggataaagt agaagaagtt
     9841 aaagaaagct taattgaaaa tggatttgaa atagttgaag ttcaaaaatt aggtgaatgg
     9901 tcagcaatag tttctaagct taaaaaataa gggatatggt gacgtataat ggatagattt
     9961 tttgttgaaa aaaataatat aaatctacaa gataaaactt gcactataga aggtgaagat
    10021 gtaaaacata tttcaaaagt tttaagatgc aaattaggtg agaaacttga aatatgtgac
    10081 aagaataata atgagtatat ttgtgagata atgaatatag ataagagtat agttaattta
    10141 gagattttag aaaaagttga tataaataga gaatcagaac ttaaagtaag gctataccag
    10201 ggacttccaa aagctccaaa aatggaaatg atacttcaaa aattaactga agtgggtgta
    10261 gaagaaataa tattagttca aactaagaga agtgttgtta aagttgatga caaaaaagaa
    10321 gataaaaaat ttgaacgttg ggaaagaata atttatgaag cagcaaagca aagcaaaaga
    10381 ggtaagatac caaaattaag aggtgtttta agttttaaag aagctttaga agatatgaaa
    10441 aaaaataatg taaatatttg cccttatgaa aatgaaagaa cagtttcaat aaaacatgca
    10501 ctaaaaaaat gtgattctaa tatagatagt gttggaattt ttataggtcc agaaggtgga
    10561 ttttctgaag aagaaataga acaaatacaa aaaaataatt gtaatgttgt atcattgggg
    10621 cctagaatat taagaactga gacagcttct gttgtagcat caacaatagc tttgtatgaa
    10681 ctaagtgatt taggaggaga aaaataaatt gaaaaaagta gctttttata cacttgggtg
    10741 taaagttaat caatatgaaa cagaagctat gctagaacta tttgagaaag atggttatga
    10801 acaagtcaat agtgaagagt atgctgatgt ttatgttata aatacatgta cagttactca
    10861 tatgagtgat agaaaatctc gtcaatatat aagacgagtt aaaaagaaga atccagatgc
    10921 gataatagcc gttgtaggat gttattctca ggtatctccg gaagaaattt tggatataga
    10981 agaagtaaat ttagtcatgg gtacaaatga tagaagaaag attgttgaag aaattaaaaa
    11041 tataaattca agtaagaaag ttagtacagt tgatgacata atgaaagtta aagcttttga
    11101 agaaatagaa atcagccaaa ctaatggaaa gactagagca tttatgaaaa tacaagatgg
    11161 atgtgacaga ttttgtactt attgtatcat cccttatgcc cgaggacgcg ttagaagtag
    11221 agatattgat agtatagtcg atgaagtgaa aaaattggct aataatggct ataaagaggt
    11281 tgtattaaca ggaatacatg ttgcctctta tggaaaagat ttaaaggata gggatattaa
    11341 acttcttgat gtaataaaac aaataaacca gatagaaaaa atagaaagaa ttaggcttag
    11401 ttctgttgaa ccaattttat ttacagatga atttgtaaat gaagttttaa agatggataa
    11461 agtttgtcct cattaccatt tatcacttca aagtggatgt gatgaaactc taaaaagaat
    11521 gaatagacga tacacaactt tagagtacaa aactattgta gatagattga gaagtaaaat
    11581 gtcagatgtg gcaataacta cagatgtaat agtaggtttt cctggagaga caaatgaaga
    11641 gtttaaaaaa acgtacgagt ttttaaaaga aatagagctt tcacaaatgc atatttttaa
    11701 atattcacca agaaaaggaa ctccagctgc aactatggaa aatcaagttg accctcaaat
    11761 gaaacacttt agaagtgaac aacttttaaa tttaagtaaa gtaaatttta ataaatttgc
    11821 aactaaattt atcggccgtg agttggatgt tttatttgaa caaaatatag aaggcaataa
    11881 atatgaggga ttaacttcaa attatataag agttgttgtt gaaagtgaca aaaatataca
    11941 aggtcagata ttaaaagtga aaataaatga tgtaaaagat gaatatgtag aaggaatttt
    12001 gctataacat gtagaaatta taatatttat aagataggag gtggaaagaa tggattgtat
    12061 tttttgtaaa atagcgaacg gagaaatacc ttcaactaaa gtttatgaag atgatagagt
    12121 cttagctttt aatgacttga acccagttgc tccatatcat atattagttg tacctaaaaa
    12181 acattatgat agtttaattg atattcctga taaagagatg gacattgtgt cacatattca
    12241 tgttgttatt aataaaatag ctaaagagaa aggttttgac caaactggat tcagagtcat
    12301 aaataattgt ggaagtgatg gagggcaaga agttaaacat ttgcactatc atattttagc
    12361 tggtaaaaag cttcctaatt acgaagcggg acaaaactaa actaaattat tgaaaaattg
    12421 agttccttct tgataaaaat acttgataaa agtaaaaatt tagtttataa taaacaaagt
    12481 gttaggttgt aatgcatatt atgcaaaaag aacatagtta cagtcccagc ataattgcgc
    12541 tattggaggg agggaaaaag aaatgtcaga agtaagagtt agagagaatg aaacattaga
    12601 cagcgcttta agaagattca agcgtcaatg tgcaatgtct ggcatcatgt cagaagtgag
    12661 aaaaagagag cactatgata agccaagcgt taagcgtaag aaaaaagctg aagcagcaag
    12721 aagaaaaaac gctaagaaat agtagtaaat aagaataaag aggtgaaggg gatgtccctt
    12781 aagcaaaagt tacaagagga tttgaaatct tcgatgaaaa acaaagatac agtgagaaaa
    12841 tctgtagtaa ctttaataag agcttctata aagcagtatg aggttgataa tagggtcgag
    12901 cttgatgaag atggaattat cgatgtaata gcaaaacaat taaagcaacg tagagatgca
    12961 ttagttgaat ttgaaaaagc tggaagagaa gacttaataa aagaaacaga aggtgaaatt
    13021 gaagttttaa aagagtacct acctcaacaa ttaagcgaag aagagttaga agaaattgta
    13081 aaatctacta tatctgaagt aggggcaact tctatgaagg atatgggaaa aataatgtca
    13141 gttatccaac caaaagttaa aggaagagct gacggaaaac ttataaataa gttggtaaaa
    13201 caaaacttac aatagattga tatagcctag agagaaatct ctaggctttt tatagttttt
    13261 gggaataacg agtcatattt gagcatatct ataatcaaag aaaattttga aagaagggga
    13321 aaaatttgat tatatcagta aaagagatta ggaatttttt gataaacata tttataccac
    13381 taataatagg ttacttaagt agcatcttaa caatgattat cagtggaaca gatatttcaa
    13441 cctattattt acaactcaca aagccaagct ttgcaccacc agcttttatc tttcctatag
    13501 tttggactat attgtacata ttgatgggta tatcttcata cctaatatta agaaagggtt
    13561 acaacttacc taaagtaaga gatgcaattt tttattatgg attacaactt gtattaaact
    13621 ttatttggag tatactattt tttggatttg gacttagatt tacagctttg atagatataa
    13681 taataatgat tttggtttct ataataatga tttcgaagtt ttctaaaata gataagagat
    13741 ctggatacat aaatttgatt tatttaatgt ggcttgttta tgcaggattt ttgaactatt
    13801 ttatatggtt tattaataaa tagaatgtaa agattttgta aattaccctg tacttttaat
    13861 aatatcataa caaacgaaaa ataaatagac tattaaaaat aatcattttc atatacaata
    13921 atattaattt aaatacaata aatttataaa aattgtattt aaagattaac atattaaagt
    13981 attagaattg gaaaggcttg aagggagagg tttttatgca aataatagta tctgggagac
    14041 aaatgaaatt aactgacgga ataaaaggat acgtagatgg aaaattaagt aggttagaaa
    14101 aataccttga tcctgaatca gaagtgaaag taacagtaag tgcgaaaaag gatagacaaa
    14161 aagtggaggt tacaataatt cctattaacg gtcaaataat aagagcagaa gatgtagaag
    14221 atgacttata tgcagcaatt gatattgttt gtgacaaatt aagtagacaa gttgtcaaat
    14281 ataaaactaa agttaaagac aaagttcaaa ataataagag cataagattt gaaaatcttg
    14341 actttataga taatagcagt gaatttgatg attatgatta tgatgatgaa gaagatgaaa
    14401 atatagtaat agaaagaaga aagaaattta atgtaaaacc tatgagttct gaggaggcga
    14461 tacttcaaat ggaactagta ggacacaatt tctatatgtt tagaaaccaa gataattttg
    14521 aaataaacat agtgtataag cgtaaagctg gtggatatgg tttaatagaa caagattaat
    14581 aaaaattgta tacgtttaga ttttatataa gaaaaaataa aaaggtaatc atgttgattt
    14641 tttatcaata tgattacctt tttattgttt aaataatata ttagatatta ggtatttatt
    14701 atataaaaat tttaaaacta atctgttaca acatcaataa agtgcataaa tacctatttc
    14761 aagcataaaa ttaattaagg gggtaatgct tatgttagaa atatccactg atttacaagt
    14821 aaatcaacca gttattactg ttacaagtaa tacgtttatt agtatagaaa attatctatc
    14881 aatattagaa tatgaagttg atttaataag aataaagact aaagttaaaa caattaaaat
    14941 atctggggat aaactttcac ttaagtatat tactgatagt gagataggta taaaagggat
    15001 tatctacaat gttgagtatg ttgattagga ggagatacac ttgataagtt ttataagggg
    15061 atactatgtt atagttgtgg aaggagtagg actcgaacaa tttttaaatc atcttataag
    15121 aaatgggata agtgtttaca atgtaactag aataaaaaat actaaaatgg agtttcatat
    15181 agatagacaa gatataaaag aatttaagaa tgtttataga ggaagtaagt ttgatataaa
    15241 ggtaaagcaa aaaactgggg tcccttttat tatcaaaaga gtttataagc acaaaggtat
    15301 gtggatttgt gctcttgttt ctttattttt attgatgtct acatcacagt ttgtgacaga
    15361 tgtatatata caatctcctg aaggtataaa aaaagaagct cttagaaatg agctttataa
    15421 ggttggagta agaccaggag tctataagaa gagtattgat agaaaagaag ttagagacca
    15481 tatgatgtca aaatttaatg atgtggcata tttatctatc aatgtaaaag gaacaaatat
    15541 atttgttact gttacaaaaa aggcagaatc cctaaaatct acagaccaat caaattattg
    15601 taatgtaatt gctttaaaaa atggtataat agaaaaggta atccctagaa gtggtaaatc
    15661 agttattaaa tctggagaca tagtccaaaa gggagatgtc ttattaaatg gagctaatac
    15721 caagtcaata ccagaagtat gggcaagtac tttttatgag tccacaaaaa aggcaagtta
    15781 tgtagatact gtaaataaaa aaactggaga aaagaaaaat atatatacac taagttttta
    15841 tgataaagag tttacaatga gaaaaaatat aaaatataaa gattatgttg ttgaaaataa
    15901 agaaaaaaaa ctatcaatag gaaactatac ttttccaata aagataaaaa cgagtacttt
    15961 ttatgaaaca aaaaaggttg aagtaaaaag gaataaagaa gagttaaaaa aagaattatc
    16021 tgaaaaagct ttaaaagagt tagaatacat aatcccagct tcggctagaa taatagatgt
    16081 aaaacataac tttaaggtta ataaaaatat gttagaatat ttaataacag ttcaaactag
    16141 tgaaaatatt gctaaaattt atcctcttag taagtcagaa gcagaaaggt ttataaagga
    16201 agaaagtaaa ccagatgaag gtgaagagga agttccttcc aatccagaaa aaagaccatt
    16261 aaatgatata agaaatgagt ttgatgagga taataaggat aataaagacc aaaacaattc
    16321 tgatgaaaat aatagtaatc aaaatagtaa taatagtcaa aataataata gcaataacaa
    16381 ctaataaaat taaacttaaa atcttaggag gactaattca attgataata caaaagaaat
    16441 ttacaatagc agatggtaac tttgaaagag agttatttgg caactttgat gaaaatgtaa
    16501 aattaataga gaagacttta aatatagatg taatacttag agaaggcaat ataattttaa
    16561 ttggagaaga aaaaaatgta gattctgcat taaaacttat gaatgaatta caccaaactg
    16621 tatcaaatgg taagcattta gataagcaga gtatttctta ttcattatct cttttgcttg
    16681 aaggaagtga acaaaaaata aaagaacttg aggggacaat cgtcataact caaaggggaa
    16741 aagctgttca acctaaaact ttaggtcaaa aagaatatat aaaacttata gaaaataatg
    16801 atataacttt tggtgttgga cctgctggaa caggaaagac ttatcttgcg gttgctatgg
    16861 cagtaaaagc ttttaaaaga gatgaagtta gtagaattat acttacaaga ccggctgtgg
    16921 aagcaggaga gagtctaggt tttttaccag gagatttaaa ggacaaagtt gacccatatt
    16981 taagaccact atatgatgcc ttatttgaaa tgttgggtgc tgataaattt aataagtatt
    17041 tagaaagagg aactattgag gttgcaccac ttgcatttat gagaggtaga acacttgata
    17101 attcatttat aatattggat gaagctcaaa atactacttc tgaacaaatg aaaatgtttt
    17161 taactagact tggatttggc tctaaagctg tagttacagg agatgtaact caaacagatt
    17221 taccacaaaa taagaagagt ggtctaatac aagctacaga gattcttaaa ggtgttccag
    17281 gtattgggtc tataatgcta acagatagag atgttgtaag acatgagttg gtacagagaa
    17341 taataagagc ttatgagaag cacgacaaaa gagaagaatt taaaaaagaa gaacgtaaaa
    17401 aagcgaaaat gcaggaaaag actttcaaaa gaaagtaaaa gtgtagtaat atagatatat
    17461 agcattatta atgaataaaa agtaggtgta gtgaatggat ttgatactag atgatagaca
    17521 agataaatta gaagtaagtg aggagttaat tgaaaagata aaggatataa tcatagaatg
    17581 tttagattat gaaggatatg atgataatta tgaagtgagt ctttcttttg tagataataa
    17641 agagattcat gaattaaata gagaatatag aggagttgat agagtgactg atgttttatc
    17701 atttcctctt ttaagtgatg attttgagga tgttgagtta gaagaagaat ccctaggtga
    17761 catagtagta tctcttgaaa gagccttaga acagagtata gagtataacc atagttttga
    17821 aagagaagta tgttttttaa tatgtcatag tatgtttcat cttttaggtt atgaccacga
    17881 tacagatgaa aatacaaaag aaatgagaga aaaagaagaa cacatattaa ataagttaaa
    17941 cataacaagg gagtagtttt tatgaaacca gaaaaaactc gtcaaggtat aattaaagca
    18001 tttaatgcag ctatagaagg gatattgtat acatttaaat ttgaaagaaa tatgaaaata
    18061 cattatttag gttcagttgc tgttcttata ataagtttgt tttttaactt ttcaaaatta
    18121 gaaatgataa tgttacttat gtccatatgt ttagttgtag ttgctgaaat gtttaataca
    18181 gctatagaaa aagctgttga tttagtaact gatgaatatc atgttcttgc taagatagca
    18241 aaagatgttg cagctggagg cgttctagtg gcagcactaa attcagtagt agttggatat
    18301 attttgtttt atgataaatt gacagatata tctggaatat taatttataa aataagagaa
    18361 tcagaattac atataacctt gatatgtata ttattggttc ttatagctgt tgtagttgta
    18421 aaagcattga cttctacagg tactcctcta aaaggaggaa tgccaagtgg tcatgctgcc
    18481 ttagcatttg ctatagcaac tgcaattaca cttatgactg aaagagttgt tgcctctact
    18541 cttgcatata taatggctgt attagttgcc caaagtagaa ttgaaggaaa aatacatacc
    18601 ttttgggaga ctattgctgg agctttgtta ggtgtgttga tagctatatt agtatttcag
    18661 cttggaatgt tctataatta aaataaatgt acataaaata tttaaaataa aacaaatctg
    18721 tgctaattaa atttttatga tataatgtag agtattaaat aaaaataact tttaattatt
    18781 gtatacagga ggagttgttg tggataacat agaattgtta aggttggcag aagatgccag
    18841 acaacattct tatgctcctt actcaggttt tagagtaggt gcagctcttt taactaagag
    18901 tggtaaagtt tatactggat gtaatataga atgtgcatca ttaggtggaa caaattgtgc
    18961 agaaagaaca gctattttta aagcaatatc agagggtgat aaggatatct ataaaatagc
    19021 tatagcaagt gataactcag aaaataatga gcaaacatat ccatgtggaa tatgcaggca
    19081 ggtaattata gagtttggtt ctgatataaa aataataacg ggatatacta aaggagaggt
    19141 gtttgagcat agtataaagg atttgttgcc aaactatttc tctggaaatg atttaaaata
    19201 aatttaaaag tataaataaa tttaagtaat taaaacaaga agataaaaag ttaggagaca
    19261 gttaatgttc aaatcaggtt ttgtcagtat agtgggtaga ccaaatgtag gaaaatctac
    19321 tttaatgaat aatgtagttg gagaaaaaat agctataatg agtgataaac ctcaaactac
    19381 aagaaataca atacaagcag tatatacaga tgaagaaatg cagatagtat tcttagatac
    19441 accaggtatt cacaaaccaa aaaataaatt aggtgaattt atggtaaagg cagcaacaga
    19501 agcttttaaa aatgtagatt taatattatt tgtagttgat gattctaaaa aaataggtcc
    19561 tggagataga aagataatag aagatttaag aagtgttaag acaccaataa ttttagttgt
    19621 aaacaaaata gaccaactag accaaaaaga tgagctattt gatataatca aaatgtatga
    19681 tagagaaggt atttttaaag aaatagtacc aatatcagca ttaaaaggaa agaatacgga
    19741 taccttaata aaagttatac aaaattattt agaagaaggg ccaaagtatt ttccagatta
    19801 tatgattaca gaccagccag aaagagtatt aatagcagaa cttataagag aaaaagttct
    19861 tcactatctt aatgatgaaa tacctcatgg tgtagctgtt gaaattgaaa aaatgaaagc
    19921 gagaaatgat aaggaaatag tagatgtatc tgctgtaata tactgtgaaa gagattcaca
    19981 taagggaata ataataggaa aaaatggtag gaaactaaaa ggaataggta agtcagcaag
    20041 acaagatata gaattgcttt taggttcaca aataaatctt caattatggg ttaaagtcaa
    20101 agaaaattgg agaaatttac aaaattatat aaataatttt ggatataatg ataaataaag
    20161 gtgggttata tggataggaa actggatgct tcccaagatt tattatatga agtaaaatca
    20221 ttaattgata ataataaagt attggagtta agagaattaa tagaggaata tcatataatt
    20281 gatatattcg atataatgga aaatttagag gaagacatga aaattcaatt atttgaagta
    20341 cttcctttag atatggcatc ttctatatta gaagaaggta gtgtagagtt ttttattagt
    20401 attttatcta aactggatgt agaacactcg aaaaatatac ttgaacttat gtctcttgga
    20461 gatatggcag acaagttgag tgaacttgaa gaagaagaaa gagaacacat aataaatctt
    20521 ttgaatcaag agaatgccga ttatgtaaaa gagttactat tctatgatga agattctgct
    20581 ggtggaacaa tgaccactgg atatatttct ataaataaag atatgacagc tcttgaggct
    20641 atagaccata tgagagaaga agctgaagaa gctgaaacta tttattatat atatgtggtt
    20701 gatgatgaag aaaaactagt tggagtattg tcattaagag aattaataat agctagagac
    20761 gcaaatatag tagaagattt gatgagtgaa aatataatct ctgtttatgt agatgaagat
    20821 agagaagaag cagtaagatt agtatctaaa tacaatttaa tagcaattcc agtagtagac
    20881 agacaagaaa aattaaaagg aataataaca gtagatgata taattgatgt aatggaagaa
    20941 gaagcaactg aagatatgta taaatttgca ggaagttcag aacatgaaag agaagttgct
    21001 gaaaaagaaa accctacatt gagagagcaa ataatatcag cacttagagg acgattacct
    21061 tggttaatta ttaccttagt tggagggttg ttggcttcgt taatactttc taacttagac
    21121 tatataatga atcctgtata tgctccatta gtatttttta tacctgtagt aattggtatg
    21181 ggtggaaata ttggaactca atcatcatct gttacagtaa taacattatc taataaggat
    21241 ttaaatttta gcaatgtggt tagagaaggt attgtaggta ttattacagg tcttttgtgt
    21301 agtataataa caggtattgt aatttacttt gttatgagaa agttagacat tgtattaatt
    21361 gtttcaatat cattatttat aaatatggtt ttaggtgcaa caataggtgc gtttatgcct
    21421 gttttattaa aaaaaatgga tgcagaccca tctacagttt catcaccaat tatatctaca
    21481 gctcttgata taactggtat agcagtttac tttataataa caacggcatt attgtcaaaa
    21541 attgtttaat aactttatgt atgatttatc ctcttcttac taaaaatata agtataaatg
    21601 taagaagggg gttttttaaa aatgaacaat atcagttggg aagtttttaa aaaaacaggt
    21661 agtatagatg catatttata tttctgtgat tgcaaaaaca tacatgaaga aattgaagaa
    21721 gtaagggaga aaaaagaaga cgatgataat cctgaacacc caggggatag tacttaaagc
    21781 tataaggtat aaggaaagtg acataatact tacactattt acaagaaagc ttggaaaagt
    21841 atctgcaata gcaaaaggag ctaaaaaaaa taagagctct ttgctttcat catcacagtt
    21901 attttcttat agcaatttta ccttaaaaaa acaaggtaat atgtacaagg ttactcaaag
    21961 tgaaataata aaaagttttt ataatatatc ctatgatata gaggcatttt cttatgctac
    22021 atatattaca aaattagttg aaaattctat acttgaaaat cagactaata ataggctatt
    22081 tatattactt gcgcagactc tttatttgta tacacaggat aatacagaca ataggtttat
    22141 aacagcagca ttcgagttaa aatttttaga ctatatagga tttaaaccta tagtaaataa
    22201 atgcattaat tgtgaatgta aaattttaca aaattctgtg tttaatatct acgaaggtgg
    22261 tatactatgt agtaagtgta gtaaactttt tgataataat ataaaattag acttaacaac
    22321 tataagtctt atggaatatg tattaagaaa tgatatttta acatgtagta aagctaaagt
    22381 atcaaaatat ataacacatg aacttgagaa tattctagac aagtatttga aagtttatgt
    22441 tgataatatt aattttaaat cattacacgt attgcaaagt gtaaaaaata ataagggagt
    22501 ggataatgat gagtagtaat ataactattg aaatggttga ttcagtcttt gaaagagttc
    22561 caggagcaac ttatgcagaa gcaaaagaag cattagtaat gtgcaatgga gatgtaattg
    22621 aggcagtaat atatttagaa aataaaaaaa atacttgtaa ttgtaaagca aaatctgcta
    22681 aagaaactat ggaagaagta tttggcaaag atggagaaaa tataaagttt caattaaaag
    22741 agcttcttag aaaaagtagt gtagtgagaa taatagttga aaaagatgga aaaactatga
    22801 tgaacattcc tttaacagtt ggagttgttg gattagcttt tgtaccatta gctactttag
    22861 ttggattatc agctgctgtt ataagtaaat atcgtataaa agttcaaaat gaagaagatg
    22921 gagaaattgt agatttaggt gaattgaatg aagaaaaact tcacgttttg aagaatatga
    22981 taactaatgc agcaaaagat gtgaaggatg tagtagttga caaaaaagaa gatgacaaag
    23041 atgtaactgc tgaccttatg aaagaagaat ctgaaaagaa taaagaagac gaagacgaaa
    23101 ataaatagat ataaagtgac ttttaaaaaa tgaagagaag ataattataa gaattatcac
    23161 taacattgac aaattttata gaatttgata ttctaaaagt tgtagaagaa ctattaaaat
    23221 agcctttcgt gtagacgaga ggctttattg ttataggagg gatttttatg aattttcaag
    23281 agatgatact ggcgttgcaa aaatactggt caaagcaagg ttgtatcatg atgcagcctt
    23341 atgatattga aaaaggtgca ggaactatga atcctaatac ttttttaaga tcattaggac
    23401 ctgagccttg gcaagtatgc tatgtagagc cttcaagaag accagcagat ggaagatatg
    23461 gtgaaaaccc aaacagacta tatcaacatc atcaattcca agttatacta aaaccttctc
    23521 cagataatat acaagaatta tatttggaaa gtttaaaaga gattggaatt gacccaagtg
    23581 agcatgacat aagatttgtt gaagataact gggaagctgc tacagttggt gcttggggac
    23641 ttggatggga agtttggcta gatggtatgg aaataactca atttacgtat tttcaacaag
    23701 taggtaatat tgaatgtgag cttgagacag gagaaattac atatggatta gaaagattag
    23761 ctatgtacat tcaagaagtt gatagtgttt atgatttaaa atggaatgat aaaataactt
    23821 atggtgaagt atttaataaa gctgagtatg aaaactcaat gtacgcattt gaattatgtg
    23881 atgcagatat gttgtttaat ctgtttgata tatatgaaaa agaagcatta agacttatgg
    23941 aaaatggact tgtaatacct tcatatgact atgtgttaaa atgctctcat gcatttaata
    24001 cattggatgc aagaggtgca ataggagtta gtcaaagagc ttcatttata ggtagagtta
    24061 gaaatatggc aaagactgtt gcagaaactt ttgtaaaaca aagagaagaa atgggattcc
    24121 ctcttttaaa ggacggtgac aaataatgaa aaattatctt ttatttgaaa tcggtgttga
    24181 agaaatgcca tcaagatttg taggcagtac actagaacaa ttaaaaaaca atcttagtaa
    24241 attatttgat gaaaatagaa tagcttttga taaaataaat gcgtatggga caccaagaag
    24301 acttacttta gtagtcgaag gattgagtga tagacaaaat gatttagaag aggaaataaa
    24361 gggtcctgct aagaaaattg ctgttgatgc agaaaataat ttaacaaaac cagcattagg
    24421 atttataaaa agcaaaggat taagcgaatc tgacatatat tttaaacaag ttggaaaaga
    24481 agaatatata tttgcgacta taaagcaaga tggaaaagaa acttcagaag tactaaaaga
    24541 catattacca gagactataa aatctgtagt attcccaaaa gctatgcgtt ggggaggaaa
    24601 aaatcttaaa tttgcaagac ctataagatg gatagttaca ttgttaaatg ataaagtatt
    24661 agagtttgat ttagaaggaa taatatcttc taatgtaaca aaaggtcata gatttttagg
    24721 tgaaagtaca atagaagttg attctataga agattatttt gaaaaattag agaaaaatta
    24781 tatagtttta gaccaaaaca agagaaaaga aatgataaga aaacaatgta tagaagtggc
    24841 aaattcttta ggtggagaag ttgaatttga tgaagattta cttgaagagg taacttactt
    24901 agttgaatat ccaactgctt tttatggaga atttgatgtg gattatataa aactaccaaa
    24961 agaagttgtt ataactccta tgaaacaaca tcaaagatat tttccagtac ttaaagaagg
    25021 aaaactgtta cctaacttta tagctgttag aaatggtgat tcttacagaa tagacaatgt
    25081 taaagctgga aatgaaaaag ttttagaagc aagacttgca gatgctttat ttttctatag
    25141 agaagatact aagagaaact tagaaagcta catagaaaaa ttaaaaactg ttgtattcca
    25201 agtaaaacta ggaactattt acgacaaaac tttaagactt gaaaaattaa gtgctgatat
    25261 acttgatagt ttaggtttag tagatgaaaa ggcagatact ataagggctg ctaaattatc
    25321 taaggcagac ttagttactg gaatggtagg agaatttgat gaattacaag gttttatggg
    25381 aaaagaatac gcaaaagttg gaggagagaa tgatgttgta agtgaagcta tttttgaaca
    25441 ttatcttcca agatttgcag gagatatact tcctaagact aatgcagggg ttgccttatc
    25501 aatagctgat aagttggatt caatagcagg tttctttgct ataggaatac aaccaacagg
    25561 ttctcaagac ccatatgctt taagaagaca agctttaggt atacttagta tacttatgga
    25621 cagaaaagtt gctgtggata taaaaatact tattgaacat gctttagaca attattctga
    25681 attagatttt aataaagaag aagttgttga gcaaataatg aatttcttca atgagagagt
    25741 aaaaaatcta tttaaagatt taggaataag atatgatgtt gttgatgctg ttttaagttc
    25801 tgatataaat gatgtttctg atatgcatat gagagcttta gagcttaata attggctaga
    25861 aaaagatgaa ttagtggaga tgcttactgc atttaataga gtttctactc tagctcaaaa
    25921 agcagaaagt gataaaattg atgaatcttt attaaaagaa gatgctgaaa ttaaacttta
    25981 caatgaattt aaacaagtaa aggtaaaagt taatgaatta ttaaaggata agaaatatgg
    26041 tattgcttta gatgaatttg catctttaag agcagtagtt gataatttat ttgatacagt
    26101 tatggttatg gataatgatg aagctattaa gaacaataga cttgctttac ttaaacaagt
    26161 atatgatact atgctagaaa tttgtgattt atctaaaatt gtttataaat aaaatttaaa
    26221 ctatttataa gaaataaggc tgtctcaaaa tagagatagt cttatttttc attgttgaga
    26281 tgatgaaaat atatttgatt tcatagaaaa agagtgtctc ataaactaaa aagttcattt
    26341 tgagacaccc atgctttttg taagtaaata taataaatta tattttatac ttatcagtta
    26401 ttttgtttaa aatattatca tttaaattta cccattcata aaaattgcct gatgatgcgt
    26461 ccgatatttt ataaagcatt tcaggaatat cactagcttt aaaatctcca agtgattttc
    26521 ccaatctagt cttacctatt tcaaaactgc ctccaacaaa taattcaaat gcatctgtag
    26581 atataccatc aacattcttc tttttaccac ataaacctat gctaccaact tgatgaacac
    26641 cacatgaatt taaacatcct gatatatata acttaggtgc tttatttaaa atttcttcat
    26701 tattttgtaa tctaaaataa tctatgattt catgtaacat tttctgacta ttttgtatgc
    26761 ccatttgaca aattggaaca cctatgcaag atatactttg ttctaattga gaattacaac
    26821 taatagtttt acttatttct aatattcttt ttgcttcatt tccatttaaa tttaaaatat
    26881 ataatccctc tgtcattcct agtctaatca taggattttc aacattatct aattctttaa
    26941 gtaaagtact taaatccttt gttaacaaaa tacctccaac tggatgtata tatacagtgt
    27001 ataaaccatc ctgtttttga ggtattagtc tagaatcctg tatatctatt ttaactccag
    27061 gtttagagta atctatttct tcaatattga gttcaagact accattttct ttttctaatt
    27121 tatagtattc tttaaatttt tctaagaata gttcttcacc aagtgattcg accatatatc
    27181 taaccctagc tctgttttta ttttcataat ttccataatc cataaagaat ttaatcatac
    27241 cttcaacaca atataaggca tctttcggct ttataagttc atcaagttct aaagcaactt
    27301 tgggttgttt acctagacct ccacctaaga aaactctgaa atatggttca tcatttttca
    27361 aagtagcaac aaatcctaag tcttgtatac tacaatgagc agtatctgtg taacaattag
    27421 aataagaaac ttttaatttt ctaggtaaat ggtatgtagt agcatttttg ataaaatatt
    27481 tatcagtagc aactgcatat ggagtaacat caaagacttc actagggtca actccagata
    27541 atggagataa tccgacattt ctaggataat taccaccacc acctctagtg aatatatcat
    27601 tttttattcc ttcttccatg atatcaacaa ttgaatttat agataaatcg tgtaattgta
    27661 tagcttgtct agttgttaag tgtatcttat ctaagttgtg tttaatagct atttcataga
    27721 ttttattaag ttgatactga gataaaactc cagcagatac tctaagtctt atcataaaag
    27781 attttttgtc tctttgagca taaacaccat atccaccaga aaatccctta tattgcattt
    27841 tatttatttc tccatttaaa aattttgaag aatattcttt aaaattaggt agttcattta
    27901 acaatatctt tttaaaatta tccattattt cacctcattg ttagtcttgt ataaaaatat
    27961 atctattgta taatagatat atatattatt atctagtcag gataaaaata taacagaata
    28021 attctattaa aagtaagaat aataaggaca tagtgagtat tttgttttta ttttacaaat
    28081 taagtataat atttttgttt tataaaatat aattaagata aatagataat tttgtaattt
    28141 taatatataa tatttagtag agtgtgggag tagcacttaa tatttgcagg aggagttttt
    28201 gtatatggga gaaataataa atgcattaga tagagcccta gacataattt tgctgttata
    28261 tcatgaaaag agagaaatgg gaataactga aataagcaag gctatgggag tatataaaag
    28321 tactgtacat agaactttgg ttactcttga aaacaaaggt tttgttatac aaaatgcaga
    28381 gaatggtaag tattggctag gaataaattt atatgcaata ggtatggttg tgggagaaaa
    28441 aatgtcattg actgaaatag taaaaccata tacgaaaaaa ttaaatcaag aatttaacga
    28501 agtagttaat gtatctatat tagaagaaag agctcaagat tctccaagaa gtatcataat
    28561 acataaagag tatggttcaa atcaattact ttcagtaaat ccatccgttg gttcaagtag
    28621 tgaatgttat tgttcagcag ttggtaaatg tcttatggca tttaatgatt ctatagattt
    28681 tgaaaaatat agaaaaacac caatacacaa gtatacagaa catacaattg ataattggga
    28741 tgatatgatg ctttttttag caaaaataaa agaacaagga tatgctatag atgatgaaga
    28801 actagaacat ggactaactt gtattggagc acctatctta gataaaaata ataaagctat
    28861 tgcagccata agtttgtcag gtccaactat aagaatgaga gaaggggact ttgagtataa
    28921 gattaaaaga gttatagaaa ctgcaaaaag tatatctgaa ctttttagat gatttttaat
    28981 ctcgataata aaaataggct atatttaaat tgttatttgt aaatatcagt ttaaatgtag
    29041 cctatttttc tttaatctaa aattaaatat tcttaaaatt taaaatatta ttgaaatata
    29101 taagcgaatg tgttatcttt taattaagca ataaaaatag aacaaggttc tatactatag
    29161 aacaaaattg atgcatatat agtatttata caaaatataa atattatata tttaaaatta
    29221 agtaaaaata ttacataaag gggagatttg agaaatggaa aaagtcaagt tgagtgttga
    29281 aagatgtaag ggatgctatc tttgtattga agcttgtaaa aaagatgcaa taagtctttc
    29341 tgactacaca aataaaaaag gctttgtaac tattaaagtc gatgaagaaa aatgtattca
    29401 atgtggcgct tgttatacaa tgtgccctga tttagtattt gagatattat aaattatttt
    29461 tattagaggg gagaaaaggt atgcaaagtt tattaattaa aggaaatgaa gctttagcag
    29521 aaggtgctat acgtggtggc tgcagatttt atgcaggtta tccaataaca ccacaatcag
    29581 aaatattaga aatgatggca tggagacaaa aagaagttgg aggagtattt atccaaggtg
    29641 aatcagaaat agccagtgta aatatggtta tgggagcaag tgcacttgga gcaagagcta
    29701 tgacttcttc atctggtcca ggattttcat taaaacaaga aggtattgca tattgggtat
    29761 cagctggttt gccagcagtt gtagcatgtg tgcaaagata tggtataggt gatggattta
    29821 taggagctgg tcaagataat tattggcaag cagtaaaagg cggtggaaat ggagattatc
    29881 atttaatcgt gttagcacct aatagtgttc aagaaaatat ggatatggca tatgaatctt
    29941 ttgaattggc tgaaaaatat atgcatccag tacttatact ttctgatgct acaataggtc
    30001 aaatggtaga accttgtata gcaccaccaa tgaaagaata cgatatggat aaagctccat
    30061 gggctgcaaa aggaacacct gtaggagaaa aaaataaagt aataactgat ataacatatt
    30121 ttgaagacca agatacatgg caaaaagggt atatagaaaa aatgagaaaa gttcaagaag
    30181 atgaacaaag atgggaagag atagaaatag aagatgctga agtggtattt gtggcatacg
    30241 gtatttcatc aagaatagca gaaggtgcag ttagagatgc tagagcaaaa ggaatgaaat
    30301 taggtctaat aagaccaaaa acattgtggc cttacccaag aaaagcattt aagaaattaa
    30361 gtgattcatt gaagggactt gtaacagttg aaatgaacat gatggcacag atgaaggaag
    30421 atgtaattat agcatgtgat aatagatttc cagtatattc agtagcaaca gctcaatata
    30481 tggcagaagt aaatcaatta atagattttg ctcaacagat tatagatgga aaagctaagc
    30541 aagaggaggt attttaatta tggctaaaaa agcaccagca ataatgatgt gtgaaaatat
    30601 gttctgtgga ggctgtggac actctatagt cgatagaatt attggagaag tattaacaga
    30661 aatggatata tgtcaagata caattatatg ttcagatata ggttgtaatc atatgtttca
    30721 atttagtatg aatgtagatg ttgtcgttcc acctcatggt aaaatgggag cagcaatggc
    30781 agctatgaaa agagttagac cagataaata tatatttaca atagctggag atggtggagc
    30841 ttatgcaata gggcttggag aaactatgtc agctgctact agaaatgata atgtaatatt
    30901 tcttgttatg aacaataata tgtttgcaat gacaggaggg caaatggctc caactactat
    30961 agaaggtcaa tatacagctt ctactccagc gggtagaaat tacgcagatc atggttcaac
    31021 atttgatgtt gtaaaagtta tgggaaattt agatatagca tatctagcaa gaggaacaat
    31081 aactaaccct aaagaaataa atacaacaaa aagatacatc aaaaaagctc tagaaaaaca
    31141 aaaagaaaag aaaggttttt gcttaataga agtgcttgta ccatgtccaa caaactgggg
    31201 attaaagcca gtagatgcta tgaagagaat tgaaaatgaa cttattccaa agtatccact
    31261 tggagaattt attgattagg gaggaaataa tatgttgaat gaaattatat gtgctggttt
    31321 tggaggacag ggagttctta cagcaggaaa aattataatg tatgttgcat ataaggcaga
    31381 aaagaaaatt acatggttcc catcttatgg aaatgagatg aggggaggaa gtgcaaattg
    31441 caatgttgtt gtaagtgatg aaaaaattgc aaatccttgt gtaaaaaatc cagatatact
    31501 attgggaatg aacagcgtat cagtagataa gtatgaaagt ttaatgaaaa aaaatggtta
    31561 tatgtttgtc aatgaatcac tagtaagtaa ggatagaaag tacagagaag acattaatgt
    31621 tataaaagta cctgttactg aaatagcaca aaatttaggc aatgaaaaag ctgctaacat
    31681 ttgcatgtta ggtgctatgg taaagagttt ggatttattt acaaaagaag aatttgaaag
    31741 tggtatgtgt gaatactttg aaaatcaagg caaaggtaaa ttcaattcta aaaatataga
    31801 agcatttgaa gctggatacg actacgaatg aatattttaa atatttaaaa taattattga
    31861 aatacttatt tttatatgtt agtatttaat taataaaata tagaacgtcg ttctggacaa
    31921 taggacgaag tctttaggtc ggaatacaag aacgacgttc tgtgtagtag aactaggggg
    31981 tgttaaattg aaagttttag taataaatcc tggtggtaca tccacaaaaa tttctgtttt
    32041 ccaagatgaa aatgaaattt tgaaaaaaaa tataacccat acaagagaag atttaaaaaa
    32101 tttttctaaa gtatttgatg aatatgatta tagaaaacaa ttgatagtag atattttaag
    32161 tagtgagaat catagtataa attcttttga cgcagtagtt ggtcgtggtg gacttatgaa
    32221 ggctataaaa ggtggtacct atactgtgag tgaagaaatg attgaggata tgagaaatga
    32281 aataaatggg gaacatgcat caaatttagg tgcattatta gctaaaacta tagctgatga
    32341 aataggagta caatcatttg tagtagaccc agtttctgta gatgaatttg atgatgtatc
    32401 gaggattaca ggaatatcag acatagaaaa agcaagttgg ttacatgcat taaaccataa
    32461 agctgtttca agaaaaattg cacaaaaact agggggaaaa tacgaggatt ataactttat
    32521 tgtagctcat ttaggttcag gaatatcgat tgtagcacat aaacaaggca aaatgattga
    32581 tggaagtggt ggtaggtctg atggtccatt ttctccagaa aggagtggtg gattacttgc
    32641 atatcctctt atagaattgt gttactccgg aaaatatact catgatgaaa tggtcgcaaa
    32701 agtaagttcg attgggggaa tgtacgatta tttaggaaca aaggacatga tagaaattga
    32761 aaacagaatc aaggagggtg atgaaaaggc aaaacttata atggatgcat ttatttatca
    32821 agtagcaaaa gaaatttcta tgtatggagc ttcactttca gggaatgtag atagagttat
    32881 cttgacagga gggatatctc actcagaaat ggtagtcaaa ggagtagcag aaaaagtttc
    32941 atatctagct ccaatagaga tagtaccagg agagatggaa atggaggcac ttgctctagg
    33001 ggcacttaga gtacttaatg gagaagaaat agcaaaaact tattaatatt taggagaaag
    33061 agtatgttaa aagattttaa taatttatta gaagaggcta aaaagcttga acctgttatt
    33121 gtaagtgttg ctgttgcaga agacaaagaa gtattaaagg ctgtgaaatc agcaacagat
    33181 ttaaacatta taaagccaat tcttgtagga gacaaggata aaatagaaag tatatcaaga
    33241 gaaattggat taaaaggtca tgaaataata ggttgtcaaa atcccgatga atcagttaaa
    33301 aaagctgtag agatagttaa aaataaggaa gctaaagttc ttatgaaggg attagtaaat
    33361 acaagtgtct atatgagagc tatattaaat aaagaaacgg gtcttagaac aggaagatta
    33421 ttaagtttgc ttgcagtata tgaattacct caatatcata aacttcttta ttgtactgat
    33481 agtggaataa atgtatcgcc aaacttaaat caaaaaaaag atataatgac aaatgcactt
    33541 cttactatga aaagtatagg catggaaaat cctaaaactg cgatacttac tgcaaatgaa
    33601 atggtagacc ctaagataca atctacagtg gatgccaacc tacttgtaga gatggaaaaa
    33661 tcgggagaaa taccaaagtg tatagtggaa ggtccaatag catttgatgt agcatttgat
    33721 gcacatgcag cagaacataa aggtattaaa agtaatattg ctggtgatgt tgatttatta
    33781 gtatttccaa atatagaagc agggaatata ttaggaaagt catggcttag atttaataaa
    33841 gctaagtggg caggtatagt tttaggagca acaaatccga taattttagg ttcacgttct
    33901 gacacagctg aaataaaaat aaattcaata gcattagcgt gcctagcatc acaataagaa
    33961 attattatta aggaggaaaa agtattatga aatatgcaaa aagaatggat atggtaaaag
    34021 cttcagctat aagagattct caaaagaaaa tagctcagaa ggtagcaagt ggtggaactg
    34081 ttatatcttt tgctgcaggg cttcctgacc caaatttatt tccaatagaa gagataagtg
    34141 aaataactca aaaagtactt aatgaaaggg gaaaatttgc cctagcatat ggacctacta
    34201 aaggtgaaga tgagttgtta gatctattag taaaaagaat gaaagaagaa gaaaatattg
    34261 atacaaaagc agaaaatatt ataataacta ctgggtcaca acaagggata gcattaagtg
    34321 ctatgatttt attagaaaag ggagatgtag tagttacaga aaatccaagt tacttaggtg
    34381 caataaatgc atttagacct tatgaatgtg attttttagg agtagataca gatgaagaag
    34441 gaatggtaac agatgattta gacaaattat tatctgaaaa ttctaacata aaggtgatat
    34501 atgtaatacc aacatttcaa aaccctacag gaaaaacatg gtctttacaa agaagaaaag
    34561 attttatgga agtcgtgaac aaatatgatg tcatagtaat agaggataat ccttatggag
    34621 aaataagatt tacaccagaa cctctaccta cacttaaatc tcttgatact aaagataaag
    34681 tattgtacct tgggtcattt tcaaaggtat tatgtccagg atttagagtt gcttggatgt
    34741 gtggtaatgt agacatcata gataaggcag aacttttaaa acaaggtata gatttacagt
    34801 caaatcaatt ttcgcaagta caagtaatag aatttttaaa gaaatacaac ttaaatgaac
    34861 atatagaaaa aataagagca gagtataaga aaagatgtct gctaatgtta gatgctatga
    34921 agaaacattt cccagaggaa gctaaatata cagaaccaga tggaggaatg tttatatggg
    34981 ttgaacttcc tgaaagtata aatgtagatg atttattaga taaagccatt gatgctggag
    35041 ttgcttatgt ttctggagag tccttctttg caaataatgg tccaaaaaat actatgagac
    35101 ttaactatac aacaatgtca gaagaacaaa tcgttaaagg tgttgagata cttgcagaag
    35161 ttataaaaaa agaactcaat tatgcttgtg aataaatata aaaagtgagg gtttaaatat
    35221 gggagaaaaa gaaaacggta taaagtttgg aaagttaatg tttcaacttg ttattatagc
    35281 aatatcttgg gaattagttt atattatacc atttatccaa tatacattat atgatccaat
    35341 cctaaaagca ttagaatgta caaacactca actaggtttt ctacttacaa tttatgggtt
    35401 aggtaatata tttggagctc ctataggagg ctggttagca gatagatttg attatagaaa
    35461 gataatttta ggttctgtat ttcttaatgg agtagtatca tttttatttg catttaatat
    35521 gaactatcca tttgcagttg ctacttgggt aggttgtgca atcacatcat tagttatgaa
    35581 ctatccttct atggtaaaaa tacttagagt tataggaaaa gataatcaag gtaaggttta
    35641 tggatttaat gaagctatgg ttggagtttc tggagtagta atgggagcaa tattcttata
    35701 tatttataca tgctttgcaa ctcctacatt gggtatgaga tgggtaatga tatctttagg
    35761 aatattatca atagcaatgt gtccagtatt atggtttgta attaaagatg tagatattaa
    35821 agaagaaaga gaagaagcag gtgaaaaaat gtctgctggt gattttataa cagtactaaa
    35881 atcaccaaat acttggctag taggaataag tatattctgt gtatattcat tcacagttac
    35941 aatgtcttat tttacaccat atataacatc tgtacttgga ggttctgtag cattatctgg
    36001 tgcattagca ataataagac agcatggtct aaaattattt ggagcaccat ttggaggata
    36061 ttgtgcagac aaattaaaat ctcctacaaa agtactttta ccaatatatg tatttggtat
    36121 agcagtaata gttttattcc tagtattacc tgcatcaact ccaatgacta tattcatagc
    36181 tttaacattt gtagtaggta tattaggtta catgggaaaa ggtatatatt atgcagtaca
    36241 agatgaagta aaagttccag ttaaatattc agcaactact ataggtatag ctgcagcttt
    36301 aggattttca ccagatgtat ttcaatttgc attaatagga cactggatag atacctatag
    36361 caataaaggt tacacatata catttatatt ccaaatagca attctagtta ttggaatact
    36421 atcttgttta tatatattaa aggttaaaaa gagaagagca agtaaattag aagcttaaat
    36481 attcaatttg gtaattaagt atatataaat acaatactta ttttgatgaa tgtgattgta
    36541 taaataacat aaaaaatatc aatagattaa gttttataat aatgtttaga gaataaagta
    36601 taaaaatcaa aatgttatca agttttctag gttttaacat aaagttaccc ctgaaggtgt
    36661 aaaattgagt ggcaattatt atctattttc ttacctacat aaacctattt aatttaaatt
    36721 ctaagagatt ttcaaaatag atggtagttg tactcaattt taaacctcat aataaatacg
    36781 aaaatttaaa atctaactag atactattta attatatatt aataaatata tattgtacta
    36841 tttatataat aataatttca ggcttttcat tatatgtatt ccaatttata ttgtttgatt
    36901 attattcaag cacacgtcgt taaaaatttt ataaatttat gtttagatat gattaatact
    36961 cttgatttta gaaataaata tttgtagatt gtagattaaa gataatataa ataaatagaa
    37021 ggtagatttt ttagatatat taatataaag attatataaa atattgaaat atagctgttt
    37081 aaattattgt ctataaatat aaaatgaatg gtggtgagtt aagtgaccga tttagttaac
    37141 accattaatg gaattgtttg gtcacccgta ttagttgcaa tgtgtttgtg tataggatta
    37201 tatttttcat tgaaattaaa attttttcaa ataagagatg taaaagaaat gtttactctc
    37261 ttgcttgaag gagatagttc tcatgttggt atatcatcat ttcaaggttt tgctacatct
    37321 atggctggaa gaatagggac tggaaatata gctggtgtag ctgtagcaat agcaatggga
    37381 ggtccaggag cattagtatg gatgtgtata atggctacat taggttcagc aactgcattt
    37441 gtagaatcta ctctagctca aatatataaa gatgagcatg atgggcagta tagaggagga
    37501 cctccatatt attttgaaaa aggtcttggg tggaaaaagt gttcagtttt attttcaata
    37561 gttgcaataa taagttatac attttttatg cctggtacac aatcaaacac tttatatcta
    37621 gcaatgaatc aagcatttgg attttcaaaa atgacaatag cagtagcatc ttctattgca
    37681 ttagcgataa taatttttgg aggaattaaa aggattggtt tatttgctga aaaagtagta
    37741 cctataatgg gtggagcata ccttattatg acattagtaa taattataac aaatattagt
    37801 ttagttccag aggctattgt aacaatgttt aaatctgcat ttggatttgg ttcagttttt
    37861 ggaggaatgc taggttcagc aatatcttgg ggagtaaaaa gaggtatata ctcaaatgaa
    37921 gcaggagaag gttctgggac ttatggtgcg gcagcagcag aagtaagtca tccagccaaa
    37981 caaggattag ttcaagcatt ttctgtttat gtagacacat tattaatatg tatggctaca
    38041 ggtattatga ttgtaataac aggaatgtat aatgttacag gtgtttgcac agcactgtca
    38101 ggtgtagaac cgggaccaca atatgtacag agtgcaataa atacattaca tccacaatta
    38161 ggtgatattt ttatagcaat agttttatta ttatttacat ttacaacttt attagccttc
    38221 tcatatatga tggagactaa tgtatcttac ttagtaacta gaattaaagc agaaaaacat
    38281 gaaaagtaca taattaatgt ctgtagattt atgcttatag catgtgcagc aatgtgttgt
    38341 tttatagaac cacttacaag ctgggcatta ggtgatatat gtataggtgt attaacatat
    38401 ataaatttaa ttgccatctt tacactaaaa aaaccagcta taaaggcata ttatgattat
    38461 ataagacaga agaagatggg aataaaaagg agtgatagaa cttttaaccc tatagagtta
    38521 gatataagaa atgctgagtt ttgggaaaat agatacttaa aagaggataa gcatgagtta
    38581 aattaaagtt ataataaaat ataaaaatta ctaaataagt ttagtgggag gtaagattgg
    38641 tatgaaagaa agaataatac aagtggctga ttcaaagaaa gaaaaaattc tagggctatg
    38701 tcaatcttta tatgatgaac cagaaattgc tttgcaagaa tataaatctg ctaaaaaaat
    38761 atcagaattt ctaagagaag agggttttga tgttgaggaa aacttggcag gtatggctac
    38821 agcttttaaa gcaaccaaga aaaatggaga tggtcctaaa atagcattta tagcagaata
    38881 tgatgctctt ccaggcaatg gacatgcatg tggacatcat ttaattgcat ctatgggagt
    38941 tggagctgga atagctcttt caagtatact ggatacttac aagggagaag taagtataat
    39001 tggaacacct gctgaagaaa ctggagatgg aaagccttac ttgatagaac atggtgtatt
    39061 tgatggctat gatgcagcaa tgatgataca tccaaattca aaaacttgtg taacacctga
    39121 aattatagca ataggaggtc tagatttcat atttactgga aaagcatctc atgcaggagc
    39181 taagccatat aacggaataa atgctttaga tgcagtagta ctactatata ataatataaa
    39241 tgcactaaga caacaattag tagatggaac gagaatacat ggtattatac tagaagcagg
    39301 aactgctgca aatgtaatac ctgatatggg aaaagtaaga ttagagataa gggcaaaaga
    39361 acaaaattac tttgatgaag tagtagaaaa agtgaaaaat tgtgctagag gtgctgcaat
    39421 agccactgga tgtgaacttg aattttatca ctttgaacca acttgccaag gattaaatga
    39481 aaataaagtt ttggtagaca tatttacaaa gattatggag gaatttggaa tatatgaaga
    39541 tgaacaagta atgttaggtt caacagatat gggaaattta agtcaaataa tgccatgtat
    39601 acatcctttg atgaagtttt ctgaaaatgg agaagaacta catacaaaag aatttttgga
    39661 ggcttctata aattcatatg caaaagatag agtaattgat ggtataaaaa tattagcttt
    39721 aacaggattt aacttatttg aaaatccaga gttattgaaa aaaatgaagg aagagtagaa
    39781 cttagactat ttttaattat actctttaaa ataagaagag tgtctcaaaa tgaacttttt
    39841 agtttatgag acactctttt ttgtatgaaa ttaaatatat ttttattatt ttaacaatga
    39901 aaaagaggct tatctgtttt tatataacct tttttaactt gtagcacaaa atatcatgaa
    39961 tgattacaat tcgagtgata aaatcgatta taagatatat aggaggggta catatggaaa
    40021 ctttagaagt aagaaatatt gaattgagtg gtacaaatta tgagatagga tacagactag
    40081 gagaattggt tgctaatatg ccagagatac tagaaggtca gattaataaa tctaatgtgg
    40141 taagcaaaaa agaagaaaaa gagatgatag agttgtttga taaatattgt ccaggtctta
    40201 atgaagaatt gcaaggtttt gctgatgcaa ttcaagtgaa ttgtaatcaa attttatatt
    40261 atactatgac ttatcttaaa ccaggttgta gtcaagttgc attagcacca gaattaactg
    40321 aaaatgggca tgtactattt gcacgtaatt tcgatttctc acacaatatg gaggattttg
    40381 ttttatgtaa aactaaggta aatggaaaat atgcacatat aggtactacc gttatgcaat
    40441 ttggaagagg tgaaggtatg aatgaatgtg gcttaggtgt aagtcagtca tcatgtggta
    40501 ttcctgtagg caattcagat ggtttaagaa aaccagcaat tgtaggttta cagttttggg
    40561 ctgttattag atatttgtta gaaaattgca aagatgtaga tgaagcatta gaatatttaa
    40621 aagatatgcc aattgcatac aatattaatt tattgcttgc agataagtct ggaaacattg
    40681 cacttgttga gactttagat gggaaaaaag aagtaaatat aatcaacagt ttagaaagta
    40741 aaagagaata ttttttacac tctactaatc atacacatat tgataaactt cataaattag
    40801 agccacaatc aatgaaaaat tctattcata gatataaatt gattaaagaa tatataaata
    40861 agtctaaaaa aattggagag aaagagttaa tgaatttatt gtcttcaaaa tatccaaatg
    40921 gcttatcttg taattactat aatgattttt ttggaacttt aaaaagtata gttatggatt
    40981 taaatatagg aaagtttaat attttatggg gtggactaga gaataaatgg gagagctact
    41041 atttaaaaaa tgacattaaa tctataacac agaggataaa cataaacatt gaaaaagcac
    41101 catctgactt ttttgatttc attgaataaa aaatgtattc aattattcta aataattagg
    41161 atatataaat caactaaaag tttttattta tcgtattgaa ttagttataa tattttagta
    41221 tagtaaaaaa tagtgctatt ttacatattt aaagagttta agtagaagag aggtgccata
    41281 ttggattact taaagtattt tgaaaaagct gtaatgtata ttgagaataa tttacatgaa
    41341 aatataactg taaatgatgt ggcaaaagaa actggatatt cttattatca tttaacacgc
    41401 ttatttaagt ctatgtttgg tgagagtgta ggaagttata taaaaaaaag acgattagta
    41461 agtagtacaa aagaactttt atatagtgat aaaaaagtga tagatatagc tattggtagt
    41521 ggatttgaat cttcagaagc atttagcaga gcatttaaat ctatatacaa ggttagccct
    41581 atagaatata gaaaaaatag aattgatgta tttgttggta aaaagaaaaa attagagtta
    41641 gattttatga gacatcttgt tggaaatata acgattaaac cagctataaa agaaatagaa
    41701 aaaataaagg taataggtat aaaggataaa gtaattttag aaaataattc attacctgac
    41761 ttgtgggaga agttcagaaa aatacatcat attgtcccaa atactttgcc atcaaagaga
    41821 gtttttggaa tctgtgaagc aatctctgaa atacacctag ttagtgaaag tatggagttt
    41881 aatgagataa taggacttga agttaatagt tatgatgtga ttccaaattc atttgtttca
    41941 aaaactatta aggaaggaaa gtatgctata tttactcata ctggtaactt agataattta
    42001 gacaaaactt atgaatatat atggggtact tggtttttaa gttcaaaaga agaacttgat
    42061 ataagagatg attttgaagt atatgatgaa cgatttttag gtcctaataa tgtgaactca
    42121 caaattgata tttatatacc tataaaataa tctataagaa aactaaaaaa t
//
LOCUS       DAEMHW010000011        40037 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEMHW010000011 DAEMHW010000000
VERSION     DAEMHW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40037
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.011"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..496)
                     /locus_tag="KPK67_000470"
     CDS             complement(<1..496)
                     /locus_tag="KPK67_000470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457478.1"
                     /translation="MTEKLTENASLGELMAALENVQTDFQTGKNNISSALGSPFIGTD
                     KFGTTKTKIETLKNVLVETINSKNVSATSSETFTNLIEKVNWIFQSIEIFSLKNRIQA
                     TTLNTPSIVYNEVSSIKGTLRFKGSLVASKWRADYATAKIEILCGSRKEYFYVTDNTP
                     SASSA"
     gene            complement(510..1334)
                     /locus_tag="KPK67_000471"
     CDS             complement(510..1334)
                     /locus_tag="KPK67_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG2457479.1"
                     /translation="MAQAQYYTLLTEIGKAAIANATALGTRVDFAKIKVGDGGGSSYI
                     PTETQTELKNVVWESTLEHVQADEKNPNWVVIQKTITGDTGSFTIREVGVFDSKDQLL
                     AISSYPETYKPAPDSGTVKEILIKIILAVSNTASINLKIDPTVVLATLKDIQDLDTKI
                     DTTKTELKSNIETAKTEIDEKIGDTTQLTTTDKTNIVSALNEVKTSVDSIETTAEKTS
                     IKDTDNLFESDNVEGALKELAENYNTLSEKQNNLETEVNGQRAKGITIANNLIDMI"
     gene            complement(1335..1952)
                     /locus_tag="KPK67_000472"
     CDS             complement(1335..1952)
                     /locus_tag="KPK67_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG2457480.1"
                     /translation="MKLIDKLPSFYNNSITRKIQEAYDIELETLRETYDDTFDQFFVD
                     TATWGLDYWENILSIKNRFDLNIEDRRSNIKAKMRGKGTTTIEVIKAIGEAYTKTNVD
                     VEVFSNLFSFTLSFITNNCSYNTILELDKKIEEIKPAHLEHKFEMILFNENGLYAGAM
                     TSTGETVTIYPYTPKNIECFGEIILASGNNKSAERVTLYPKQEVI"
     gene            complement(1952..3001)
                     /locus_tag="KPK67_000473"
     CDS             complement(1952..3001)
                     /locus_tag="KPK67_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG2457481.1"
                     /translation="MYSSQTYDVIKNRTLSNIDLDIYKGEGSFLSDMVSPISTELAKF
                     YIELSYLHKKAFIEDNFDDFLDKRVNEFGVYRKLGTEATGEVVFEGKAGTVVQNGTIV
                     SYNELLFVVIKDIVISSEIEQNTSPVQALEIGIRYNIPANTEFKLQDEINGITKIYNN
                     LNFKGGTEIETDEELKERFYKIQKNQATSGNKAHYEAWALEVEGVYNAKIYPRWDGPG
                     TVKVLIFGENNQAVDTEVIERCKEHIEIEMPIGPALTVATPSLLDVSISATIKLEAGY
                     TIDFVKESFLESINSYLINVNKEIIYTKVSAILASIEGLHDFSNLLLNNKAENIVFEE
                     DKVPSVTTLNFSEVV"
     gene            complement(2994..3422)
                     /locus_tag="KPK67_000474"
     CDS             complement(2994..3422)
                     /locus_tag="KPK67_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG2457482.1"
                     /translation="MSTIFPFIGVPADYEIPKTQELELFREVAWNFEKDEPILENGDF
                     KIVEGNEAIKVWVYKCIKTNRYEHEIYSWDYGTELSELIGQKYSKGLTESEASRYIKE
                     ALLVNPYILDVNIKNTKFTDDLLSVDIVINTIYGEVEVNV"
     gene            complement(3422..3748)
                     /locus_tag="KPK67_000475"
     CDS             complement(3422..3748)
                     /locus_tag="KPK67_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG2457483.1"
                     /translation="MANPINEFIGIIREEGKYYNEPSFFIGEIKSKLPDLKIEINNII
                     LEKEDILIDSWMLDRQIESFDTETNQEHQHEVKNPFIDKFESGDTVIMFKIGDKFAVV
                     SKLVSL"
     gene            complement(3764..5287)
                     /locus_tag="KPK67_000476"
     CDS             complement(3764..5287)
                     /locus_tag="KPK67_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG2457484.1"
                     /translation="MIINRSKDSSNNSISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFNDNKLAVGNIPKTGVKHTKMYIGATGYDTIMSAYTEASKTTKKKYMIEANLDKFN
                     VIEKGIVTLNVMFEEGSNLINTSFSESMENVKNKVLVVDQYGNKISEKVNDEIFKDVG
                     VIMQKVIQQQENQTADIDSEFKGIEQTCSLKGYGDVSCITGRGVKVKDSYTGLVGLFY
                     IDTDKHNWDSSGNYEIDLDLNFQNIMDEKTAGQDEQKEESSDFSSGEGTLNGREVKAE
                     FTAYYPSNNPMEGGYYQAMDGKRLVPSNNTCAAPSKLKFKTKIQAKCPGTKIDGKTYT
                     VTDRGGAIGLKNGVYRIDILMSSEKECNDFGRRKGTIIIGDGTGYTNATGKAKELISI
                     AKSKLGCKYVWGATGPNTFDCSGFTQWCYKKIGINIPRVSRDQGKTGKAVSKGSLQPG
                     DLVFFSSKGANGAIDHVGMFIGSGEFIHSPHTGDIVKVSKLSGSYYAKNYVTARRYL"
     gene            5588..5788
                     /locus_tag="KPK67_000477"
     CDS             5588..5788
                     /locus_tag="KPK67_000477"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457485.1"
                     /translation="MYDFKPVTGYCPFLEKDITVTCKFLLVHKPRELNPGKKFMGHNC
                     SQEKCTYDGKSCPLANLGRETY"
     gene            complement(5789..6097)
                     /locus_tag="KPK67_000478"
     CDS             complement(5789..6097)
                     /locus_tag="KPK67_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457486.1"
                     /translation="MDINNSLSEVIELQKVRIIPENYGLTKDDLTEKDLYCMAKHIQI
                     NVIKRCFREEHDILDPCQTCKYERECFKSGYGYAHWDTFIKLSKITGVRMCPGAGFID
                     "
     gene            complement(6076..6711)
                     /locus_tag="KPK67_000479"
     CDS             complement(6076..6711)
                     /locus_tag="KPK67_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG2457487.1"
                     /translation="MEMWIRQANDTFRFPVFPSSFEINSKAIVNTSNVLKAGEIAVFG
                     GVGLRTTEISSFFPKNDYTFCDYTGFPSPYDCVNKIEKWMNEGFILRFTVTETNINME
                     VIIDSFKYEEKDCTGDVYFTLNLKEYKRIQIPKVNTNNDEKLSSVKDVPLTKGFDTKQ
                     KTHKVGKGDSLWSLAKKYYGNGDLWKKIYDANKKIIKNPDLIKDGWILIIP"
     gene            complement(6729..6920)
                     /locus_tag="KPK67_000480"
     CDS             complement(6729..6920)
                     /locus_tag="KPK67_000480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457488.1"
                     /translation="MNKDIEFIACSMRLKILIQAKENLIEDIHEYSNNEEDIARYENL
                     DKAFEKTIIDEAKFLISLE"
     gene            complement(7413..7562)
                     /locus_tag="KPK67_000481"
     CDS             complement(7413..7562)
                     /locus_tag="KPK67_000481"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457489.1"
                     /translation="MGDNLLNNVCNDEKEYLISYLKTLKQEDEHDFYVFKQIVDIYCK
                     QAKYK"
     gene            complement(7562..8383)
                     /locus_tag="KPK67_000482"
     CDS             complement(7562..8383)
                     /locus_tag="KPK67_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HBG2457490.1"
                     /translation="MNEIMNFEGKEIEIFEYNGQVLFNPYDCGRCLELSDSAIRNHLS
                     KMNDTQAILLKNSAVLDTDIRKLNNAGEKFLTESGVYKLIFKSQKEEAEKFQDWISDE
                     VLPAIRQTGAYITNNANPDKLREKASEIEKLQLAYNSTSMLKELLDGAGFDNKSKLLT
                     AKTLYKKAGIDLPIEIDEEEHYFDTKQIASKLKIYSKSNKPAQLAVCEIIKKIDLEEN
                     EVKGVWETNGSWTGTVNKYTKSVIDKVRTWIEENNRPAKIQGEKKNFHVVYKEAM"
     gene            complement(8503..8670)
                     /locus_tag="KPK67_000483"
     CDS             complement(8503..8670)
                     /locus_tag="KPK67_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457491.1"
                     /translation="MPSKKIGRPTDNPKGSRITIRLDEESKNILEKYCEKEDIDKAEG
                     VRRGIKLLKDK"
     gene            complement(9199..9765)
                     /locus_tag="KPK67_000484"
     CDS             complement(9199..9765)
                     /locus_tag="KPK67_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBG2457492.1"
                     /translation="MENSKDEEGNFSNSEMVMLEYIGGHPLLNKEEFLFVVVKDNKIL
                     FKKSGKKSNDKMIDVFEVSYSEIKSVSIEKEEEVIRRYTATRIALFGPFALAMKKKTV
                     DKKEYLIVECKDFILSFKKNDNVCATIYKKLVEYRKNNKVEDANNIADKNNVIDPLEK
                     IKTLKELLDMGAITEEEFNTKKKELLNL"
     gene            complement(10022..12877)
                     /locus_tag="KPK67_000485"
     CDS             complement(10022..12877)
                     /locus_tag="KPK67_000485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBG2457493.1"
                     /translation="MSAGSRALEAVIRMRDEASRTLRQVRDATRALQNQTNSTSQAQE
                     RLQEQFRKVSNAAKIAGAGIVTGIGAGLVSASKAGAEFETAMTKTSTMFGDTKVDTEN
                     LNNKVLELSKNTGIAASSIGESLYNALSSGIPVTKDMGSAMDFMTKNAKLSKAGFTDI
                     DTALTATAKVLNAYKMDVSETDRVHKVMMQTQNKGITTVNELGSALAGVTTTSSAMGV
                     SFEQVGAALASMTAQGTPTSEAVTQLNGLIADLGRNGTLANKSLLSATKGTKYAGKSF
                     KELMQAGVPLNTVLDLMDASAKKSGKSLIDMFGGIDAGKAALALSGQNSEQFTNNLKA
                     MSTQADVVGSAYAKMSNTLESKIGILKESFKNLGIEIYSKLKEPLKNAAETGIKCLQD
                     LNNQFSNGSLKEGVSQIAQSFGDLTSTIIKIATKALPTMIKSLSWVLKNGPTIASILV
                     SIKVASIMTSVVKGIVTLVNAWKAAKRAAVAYSIALAFSNSAGTTAALGMTTFQIIVS
                     VLTKQMKVGAAAAALFGKASLLLGGPIGVVIVAVTTLVAGFLVLWNTNKGFRDFVINA
                     WNNIKETATKVWGGICNFFTQTIPQAWESLCTSFSNAGQWFGEMWNNIKQAFINGWNA
                     IVAFFTQTIPTWINNIGVWFGQLPAKIGYGLGFALGKIISWGISVWTYLVTNVPIWIN
                     NVVTFFAQLPNKIWVWLVSTVQKIGQWGIAMLTAAQIYSRMIINNIITIFTTLPNRIW
                     TWLTNTVQKVVTWGRQMATKGKEGAKKLINTVVDTLKSLPKKVMDIGKNIVKGLWNGI
                     AGAGGWLKGKVNDFAKGVIDGFKNGFGVHSPSVIMEKIIGINLPKGIWVGIKKELPNL
                     KNNINNVISNLTQRMYNPKEIKESNYASKYKEAITQRTQQNTINKTDSKTTNNKENNN
                     IAININWGGVIVKEEADMTKLTKMLVREIKLNLAGGV"
     gene            complement(12882..13028)
                     /locus_tag="KPK67_000486"
     CDS             complement(12882..13028)
                     /locus_tag="KPK67_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457494.1"
                     /translation="MYLMFRYHNMMPSDFFKLKYGEKHVIRAFMYQEMDEKIEEIKSL
                     GRGL"
     gene            complement(13055..13522)
                     /locus_tag="KPK67_000487"
     CDS             complement(13055..13522)
                     /locus_tag="KPK67_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457495.1"
                     /translation="MSENKLEKEMIDKKEVTEVKNVVDLLLSLDADKVKMPSITHTMF
                     CKKLGIDVSFECKAVEPEFFDELQTSGLKIEKGSLKDLDNFKMKSNVILASCNLFKDD
                     KLLKHFQSPTPKELLRRMLLAGEINELYDKICELNGYRDDNKKDKEIEEKIKN"
     gene            complement(13548..13979)
                     /locus_tag="KPK67_000488"
     CDS             complement(13548..13979)
                     /locus_tag="KPK67_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG2457496.1"
                     /translation="MATSYEPRNVINGTYGEVWINNEQIAECKAMKAEIKFDKAEIVR
                     PRKIIKGQKIIGASAEGSLTLYHVDSKILDYVTQIIKEGREPKFTITSKLSDPDSFGT
                     ERIAITGVSFDGLTIIDWENGKEGEKEVSFTFEDYNPIQTI"
     gene            complement(14001..15071)
                     /locus_tag="KPK67_000489"
     CDS             complement(14001..15071)
                     /locus_tag="KPK67_000489"
                     /inference="COORDINATES: protein
                     motif:HMM:NF016845.1,HMM:NF037565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG2457497.1"
                     /translation="MAGLVNINIEFKELATSFIQRSQTGIVVIILKDTTKMHKELTSE
                     DDIPTSLSDDNKKYVKYAFIGATDNEKILKPSKVIISTFTEDGKVEDILEELESVEFN
                     YLCMPEAIEAEKTKIVTWIKKIREEESTEAKVVLANIKADNEAVVNFTENVTIDGEEI
                     TAEKYTPRIASLIASTPNTQSVTYAPLNEVESITKIDKASADAKVQSGELILRRLSGA
                     IRIARGVNSLTTLTQEKGEIFQKIKLVDTKDLISKDIKDIYVKSYIGKVPNIYDNKCL
                     FIIAIQAYLDELAKQELIDSNFSVDIDIEKQKEYLEGKKVDTSKMKEDEIKEANTDSS
                     GFYLIKLKLVDAMEDINISVQM"
     gene            complement(15072..15524)
                     /locus_tag="KPK67_000490"
     CDS             complement(15072..15524)
                     /locus_tag="KPK67_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457498.1"
                     /translation="MLSYNEILDSFTKTIDNNFKETIVVGEYNIQDNKKSCFFVQILP
                     EMVQTATKKTDIKSFLVDIKYLPKWGKKKTDLLDILNKLENIFTRNIKVKDRYLTFSK
                     KSGSIEKDEIGNYVQFLISINYHEQIYFEEEKYELMEELNMRFEGRSD"
     gene            complement(15535..15945)
                     /locus_tag="KPK67_000491"
     CDS             complement(15535..15945)
                     /locus_tag="KPK67_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG2457499.1"
                     /translation="MIEFNSLDDLIRDFEREEKEMAKNLRRAKNNVGNQLLRKVKPKT
                     PVAEIDGGTARKSWKYKELSLFDSVVSNNVEYIYHLEYGHRTRQGTGTSENYRPKPDG
                     ISFVPGVFMLARSVDEMSSIIDNELNQIIIDFWN"
     gene            complement(15945..16295)
                     /locus_tag="KPK67_000492"
     CDS             complement(15945..16295)
                     /locus_tag="KPK67_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457500.1"
                     /translation="MTEADILALTYFCKMTIRRCVSIKNEETGVTDFNENVVITEDVP
                     CGLNGNIPNIIDTDMTSSISAFELYCRPEVDLRVGDILDITLESGNVETFIASKPFPH
                     LSHLQTNLTLKERY"
     gene            complement(16297..16644)
                     /locus_tag="KPK67_000493"
     CDS             complement(16297..16644)
                     /locus_tag="KPK67_000493"
                     /inference="COORDINATES: protein motif:HMM:NF016990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBG2457501.1"
                     /translation="MLDNIKLILNLKDDTHDSLIELYIKKYTTLVLAYCNIETLNDTL
                     ENIIEDKVIVKLKETLLSNNSADNGKISSVSRGGYSVTYNVATAKTTDELIEIKLSQK
                     DKNILNNFRKVRW"
     gene            complement(16673..16894)
                     /locus_tag="KPK67_000494"
     CDS             complement(16673..16894)
                     /locus_tag="KPK67_000494"
                     /inference="COORDINATES: protein motif:HMM:NF019139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HBG2457502.1"
                     /translation="MYRLIRDNIERIVNDTSEKEKLMYNGFKLLEDKKNVNIENLGIE
                     ELKNLAKENSIEGYSKLKKDELIEKLNNI"
     gene            complement(16908..17846)
                     /locus_tag="KPK67_000495"
     CDS             complement(16908..17846)
                     /locus_tag="KPK67_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457503.1"
                     /translation="MANVIQYASTIQKALDDSAIQTLLTGWMDANAGKAIYNGGKEIK
                     IPSLEVDGLGDYNRGSSDGYANGDIKFGYETKTMTQDRGRKFTIDPNDVDETNFTLTA
                     SEIMGKFQRTKVIPEVDAYRLSKLATVAMNVENDTNVEYGYTPHKDTVISKIKKGITT
                     IRETGYTGELVIHLTYDTLTAVEEKTLEKLMAIDFSKGGITTKVPTIDFCPLIATPQN
                     RMYSSITLYDGKSEGQKQGGYVKGSKALDCNFIIAGRDVPIAVTKQDQMRIFDPLTYQ
                     GGNSWAMDYRRYHELWVTNLNSNLVYANFKDAKPTV"
     gene            complement(17869..18468)
                     /locus_tag="KPK67_000496"
     CDS             complement(17869..18468)
                     /locus_tag="KPK67_000496"
                     /inference="COORDINATES: protein motif:HMM:NF018515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG2457504.1"
                     /translation="MEWLRKILEGIKIEENKLDIEEILKNVNTEFPKHAVPKETFNKV
                     NEQLKEADKTIKSFNSKMTQEDVEKLKTEHQTEIKKIEENHKLEVEKIQNESLKTRKL
                     SAVEKALLTNKAKHTDLLTNKFDLEKITIGEDGKIVGIEEQLKGLQESYKDLFESDTT
                     ETTKQTNAQSFYKYVPGGSGEASSEITAEQIKAAVNGQI"
     gene            complement(18590..18820)
                     /locus_tag="KPK67_000497"
     CDS             complement(18590..18820)
                     /locus_tag="KPK67_000497"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457505.1"
                     /translation="MKEYVIWFKSGNCVSGIVDEEVADKLMQDFMEADSDCRYLKGYL
                     DEDGTTIIDLSQIEAISINNCNENNNIGFSKS"
     gene            complement(18870..19142)
                     /locus_tag="KPK67_000498"
     CDS             complement(18870..19142)
                     /locus_tag="KPK67_000498"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457506.1"
                     /translation="MFKKRYIMKPSKISSRNIIAFVITVIGIVLGVFIGINIIMAHVL
                     GIANMVDNNTFTCVRLVYGLVGVISGYLIGKAIYLIAALISCIIYE"
     gene            complement(19162..19728)
                     /locus_tag="KPK67_000499"
     CDS             complement(19162..19728)
                     /locus_tag="KPK67_000499"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457507.1"
                     /translation="MARFVKKAVEVEAFKFDIDVEPPIWIRKEIERGNAWVEHSIDKE
                     HTKMFIRTLEGLIYQVNIEDYIIQGAKGEIYPCKADIFEMTYEKVEYTATIENLTNYA
                     ENLEQGHRYIDKEKVKKNSLEFSAKLKLDTKDFDKNIENTTKEIETLDEAVDRLEKKL
                     NRIFAKENKVDIDKIVKQLEERLRESIE"
     gene            complement(19796..20836)
                     /locus_tag="KPK67_000500"
     CDS             complement(19796..20836)
                     /locus_tag="KPK67_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBG2457508.1"
                     /translation="MNNNIEYWKEREKQRLNARLKDEKEVLKELDKQYKIAMKNIEKE
                     IANLFYKYAKQNKLTYAETQKNLTNNEFKTWRMDIKQYIKLIEQTGDERLLLELNTLA
                     MKSRINRLEELFYQISKEIYNTFDIQNNRVEKLLEESVKDSYYKSIYETQKHVGVGVS
                     FARLDREALKDIITYPWSGKNFSQRIWKNRDLLSEVIKEEITQMIIRGESLKKIANRV
                     SEKMDSSYENAIRLVQTEHSHFMSEADKKAYESQGVDKYQFLATLQDNTCKRCRNVDM
                     KVYLVKEAKEGENYPPLHPRCRCTTIPYFPHEEGEMRTARLPKGKTYEVPANLTYNEW
                     YKEYVIKNIEVV"
     gene            complement(20849..21199)
                     /locus_tag="KPK67_000501"
     CDS             complement(20849..21199)
                     /locus_tag="KPK67_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2457509.1"
                     /translation="MTIKVALKFFYRKLELKSGLLETEFRTSFDKLIKAILYFLGVTD
                     YKKIQQTYTRNMMSNDLEDADIATKSVGIIPTKIILRHHPWVDDPEEAERLYLEEKKI
                     QASKVSDDYNNFTE"
     gene            complement(21368..22045)
                     /locus_tag="KPK67_000502"
     CDS             complement(21368..22045)
                     /locus_tag="KPK67_000502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457510.1"
                     /translation="MKIEGTRFKLLKEYSDYPSENSKKRTRMLEVQCECGNIIKRPKS
                     QILSGHTKSCGCLVRDILLERNTTHGLGKEECRAIFYSMHRRCYNPNHKSYKNYGGRG
                     IKVCGEWHDIKNFVSWCKENGYKKGLQLDRIDNDGDYEPCNCRFVTVKQNLCNKRNTI
                     MKDGMYFDEWLECLSNKHGILKNTLRSRYYYMKKNGYSEEDICENSIVNYKRKKRQSI
                     TNPSEKS"
     gene            complement(22158..23210)
                     /locus_tag="KPK67_000503"
     CDS             complement(22158..23210)
                     /locus_tag="KPK67_000503"
                     /inference="COORDINATES: protein motif:HMM:NF016988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2457511.1"
                     /translation="MELEKIRAIISADAARRQEILQAKSYYYNKNDILKKGVVVQNRD
                     ENPLRNADNRISHNFHEILVDEKASYMFTYPVLFDIDNNKELNEKVTDVLGNEFTRKA
                     KNLAIEASNCGTAWLHYWIDEEYSGEQVTNQTFKYGVVNTEEIIPIYRNGIERELEAV
                     IRYYIQLEDVKGQIQKQAYTYVEFWTDKILDKYKFFGVSCYGSQIEHITILHRFNSVP
                     FIEFANNIKKQSDLSKYKSVLDLYDKIMSGFANDLEDIQQIIYILENYGGEDTAQFLN
                     ELKRYKAIKTETDSEGDSGGLKTMQIEIPTEARKIILEILKKQIYESGQGLQQDTENF
                     GNASGKNAMPPMLEIA"
     gene            complement(23225..25618)
                     /gene="terL"
                     /locus_tag="KPK67_000504"
     CDS             complement(23225..25618)
                     /gene="terL"
                     /locus_tag="KPK67_000504"
                     /inference="COORDINATES: protein motif:HMM:TIGR01443.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBG2457512.1"
                     /translation="MDKKLIQLEAKKELARREFFYFCNLLAPNFYKEDRAYLVETCNK
                     LQDFYYSDDEVLIINMPPRHGKSRSAGLFVEWILGKNKNEKIMTGSYNETLSTMFSKN
                     VRNAIQEEKADIDTIIYSDIFPNTKIKHGDGAMNLWSLEGGYNNYLATSPSGTATGFG
                     CSLMIVDDLIKNAEEAYNENVLEKHWDWFTNTMLSRLEEGGKIIIIMTRWSSKDLAGR
                     ALEHYKEEGKKVRHINMKALQEDGNMLCEEVLSLNSYKSKVRAMGEDIASANYQQEPI
                     DLKGCLYTKFKTYDNLPVDEKGNILFTSIKAYVDTADEGADYLCSIVYGVYNKEAYVL
                     DVLYTKESMETTEYKTAKMFYENEVNKADIESNSGGRAFARNVQRILKEKFKSNKTII
                     KWFHQSKNKNARILSNSSWVMEHIYFPVNWRDRWQDYYKAMVSYQREGKNKHDDACFE
                     EGTQISTLFGNKSIEKIKEGEYVFTPFGLRRVLWSGCTGEKETINKLGLKATRNHKVF
                     SYINGFISLDKLTGISETSIISLKELMLWKYKKLLYSMEKSIDLWDKRSIILVSQVKM
                     KKENMLKDFMWQFGSLIIKGKFQKAIIFIIKILILLITTLTTWNVFRINNTYRYMQRK
                     ILKMKNFTKQLLTTLIRQEKKLPNGIKVKKVENGTQSMQNRFQKILKYINVSVKNVGA
                     ILKAKYKHLNSVQINAEKDGEVKTDELNIHQNVLYVEQNLLEQNTRHLPKKDKHVQSH
                     VQTDSTTLIESKKVKVYNLTVEGNHVYYAHGFLVSNCDAITGVAEKALKGQGLSVFK"
     gene            complement(25611..26066)
                     /locus_tag="KPK67_000505"
     CDS             complement(25611..26066)
                     /locus_tag="KPK67_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2457513.1"
                     /translation="MAKYEYWITKEGLIKIEGWARDGLTDEQIAFNIGINVKTLYDWK
                     KKYSNICNALKKGKEVIDRQVENALLKRALGYEYDEITYEEGQETKRVTKHVVPDTTA
                     QIFWLKNRKPAEWRDKQIVESTNEITINNPFKELSTEELKRLAKLDDDG"
     gene            complement(26131..26352)
                     /locus_tag="KPK67_000506"
     CDS             complement(26131..26352)
                     /locus_tag="KPK67_000506"
                     /inference="COORDINATES: protein motif:HMM:NF025245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457514.1"
                     /translation="MFNQAEKIVEVYWGKCTVMTIQLQNGFTLVGHSACVDPANYDEQ
                     IGLNICEEQIKNELWKLEGYRLQCELGKL"
     gene            complement(26468..27078)
                     /locus_tag="KPK67_000507"
                     /pseudo
     CDS             complement(26468..27078)
                     /locus_tag="KPK67_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(27078..27251)
                     /locus_tag="KPK67_000508"
     CDS             complement(27078..27251)
                     /locus_tag="KPK67_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457515.1"
                     /translation="MATKSILKNVDVRKKAFGRNLVSALENAKNKQEKEVVLSKKCSE
                     VPKDKIKDIFGRF"
     gene            complement(27602..27784)
                     /locus_tag="KPK67_000509"
     CDS             complement(27602..27784)
                     /locus_tag="KPK67_000509"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457516.1"
                     /translation="MGILEGATKIRDIAKRIAIEKKITEQEAWDDAIKSLTMQVLVFN
                     LSSILRTCDIIYVSPY"
     gene            complement(27853..29001)
                     /locus_tag="KPK67_000510"
     CDS             complement(27853..29001)
                     /locus_tag="KPK67_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBG2457517.1"
                     /translation="MEINFNGERLKKARIYRGMTVAELAERIDCQRQTVSMYENNKSK
                     PNDNNVVKRIAKELGFPVKFFLETGNNIAIGSTYFRALLTTNKKYRAEQIQKMEFLAE
                     IYFFLQDYIEFPTLNLPDCSGKTPEEAALLLREAWGLGLKPIDNIIYEVEQHGILVTS
                     FSTSTDDIDAFSQMVDISGETVYLIGYSSNKTSASRIHFDIAHELGHICLHEWSEDVE
                     ALEKQEFKDRESEANRFASTFLLPEETFKIDAKRTPLRIPNYTELKRKWKVSIQAMIR
                     RSYSLGIISMDEYQSMIRTLQRRGLRKSEPLDDELLTSLPALLKTAVLMLLNEKVFTP
                     KEFMDELSFSYNFSLEPEEVEYLLNLPKNTLTSAKVIPFPDLQLKKDV"
     gene            complement(29035..29790)
                     /locus_tag="KPK67_000511"
     CDS             complement(29035..29790)
                     /locus_tag="KPK67_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457518.1"
                     /translation="MSNKIKLEVPNEIGYIIVQCFNKAKEEIREYIGKQNLITKNGVP
                     FQFWDLLNDAIDTAFNSSNFKAYKSKRGRWRMIFIYDKNSKYLYVLMREQRYKELHNN
                     VNKREKMHYVDILTKVFNGDLKAPIEQLSFFSLEFDNEDKIEIYLQKLIDSINKDGAI
                     IEHHVLLLFNQTAEYELTSIRAVMVDTNLNIVSEQNLSSYISIEESTVVEKVDKTNPV
                     ANNPTLGLKLTAKADARKKTHPLKKAKKEEDAK"
     gene            complement(30104..30586)
                     /locus_tag="KPK67_000512"
     CDS             complement(30104..30586)
                     /locus_tag="KPK67_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457519.1"
                     /translation="MNKKTLFQEVEGRLYNYKKLESQIRIKDIYIKKLENEFCGCKAQ
                     SYEEKTGPTYNISSSVENEVIKREEDLNRLKEDKKTLEIEKETIECALTSLNSFETEF
                     FNEMYMNNEKINMDYMSNAMHIDRSHCFRIRKRIVCKIMDMLYPKIKEFELPIFSWKV
                     "
     gene            complement(30747..31601)
                     /locus_tag="KPK67_000513"
     CDS             complement(30747..31601)
                     /locus_tag="KPK67_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HBG2457520.1"
                     /translation="MNENINKEITVLGTLEIEGMKFHNIEGGFGEHKKAMLVKDIAEI
                     HNRELKHINELINNNIKRFKTNIDIIDLKVSRSERLGDLKANPSKGLGCEDVGYSKQS
                     FNQSRNIYLLSERGYSKLLKILEDDIAWEQYEKIVDGYFSMRKELNNPLLSASKELQA
                     IFMLDKKQEVLETKIENVNEKLENFMDDAPLFNIECESIVKEVKRVATKSLGEHGSKA
                     YKNKSLRGKVYSDIYHQIKREFGVDSYKAIKRCQLDKVLEIVNNYKLPIVFEEEIRLL
                     NSQLSIVS"
     gene            complement(31703..32146)
                     /locus_tag="KPK67_000514"
     CDS             complement(31703..32146)
                     /locus_tag="KPK67_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG2457521.1"
                     /translation="MKINFVIDGEPKAKARPRMSTANGKAYTPDQTVLYENWIRLMYN
                     STVKHFFEGNVKMTVICYFDITKKDREALQKNKVNTKAYKDAIAKVEGLIRPSKKPDL
                     DNIIKSVADSLNGIAYKDDSQIVEVVSKKYYSDRPRVEVELEDII"
     gene            complement(32271..32612)
                     /locus_tag="KPK67_000515"
     CDS             complement(32271..32612)
                     /locus_tag="KPK67_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HBG2457522.1"
                     /translation="MANIYCENYNCKNYFEDMCMLERLEINNLKVCESYIEGKNELYK
                     LENGYTIHPKELKMVKNKDYSVEVTHIPTDITVKCRSTNSILKNKNKCLEVLEEELIK
                     INSHLELEDLR"
     gene            complement(32693..32875)
                     /locus_tag="KPK67_000516"
     CDS             complement(32693..32875)
                     /locus_tag="KPK67_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457523.1"
                     /translation="MNKRIKMEKGLIHKKCDERCVNYDFVISNNLITCNVCIGCKYKE
                     NMDKVCEENYKKLRSK"
     gene            complement(32878..33252)
                     /locus_tag="KPK67_000517"
     CDS             complement(32878..33252)
                     /locus_tag="KPK67_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457524.1"
                     /translation="MYSTYKVKLKTKKTLEQLRNQPDINFGSSEEGLKRALKYNNLID
                     GLEVTVIKFKDVYCLANCDEKDVEKIRDAYYILEQDEYFGCYINEYERFKKDWENGSC
                     DGEASMVFSDDEIEIIEKLREG"
     gene            complement(33245..33412)
                     /locus_tag="KPK67_000518"
     CDS             complement(33245..33412)
                     /locus_tag="KPK67_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457525.1"
                     /translation="MTNKEMCESKNIADTESYKRFSKEICGSCKNTKIDCESKDCDTA
                     YKNWLEDIYYV"
     gene            complement(33421..34419)
                     /locus_tag="KPK67_000519"
     CDS             complement(33421..34419)
                     /locus_tag="KPK67_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG2457526.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDMDLFFQKKISKVSR
                     DNDIRTAVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYTEKDEKKVAILKLDYNNSYTHSIGFEDDKFNIQMSKNEINIQETKAIKIGAIIG
                     LSGMNDEYHLSVLDKDAEKEETNSKFVTEFLNATKVKDDKYKTKVFKGLVDAFIANLY
                     SDMKQGEDVRSILLYMLREKQKLDINEFADKVIKDDLKDSFKDHAEEKGLDGSFNIDK
                     KWVEKNLKNRHIKTDTGIEIKGKMDDFEDFMKYGIRHNGNGTVDIIIKNVSFYNEK"
     gene            complement(34433..34624)
                     /locus_tag="KPK67_000520"
     CDS             complement(34433..34624)
                     /locus_tag="KPK67_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457527.1"
                     /translation="MLICVMVKTNKKGSYTHGEIKVDDDDLKGMSDEEKTEYMKKIAQ
                     EYICENLIDWYWFGIGENN"
     gene            complement(34791..34973)
                     /locus_tag="KPK67_000521"
     CDS             complement(34791..34973)
                     /locus_tag="KPK67_000521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457528.1"
                     /translation="MTNFEMIKNMSKKELAEFINEIGDNCTCIYCSCQYRYEICDVDE
                     KCVDGCKEWLDMEVEL"
     gene            complement(34966..35124)
                     /locus_tag="KPK67_000522"
     CDS             complement(34966..35124)
                     /locus_tag="KPK67_000522"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457529.1"
                     /translation="MDDRLEICKKMFPNVTKNTRLLCHYCDGRNICSYDEEKARKLLR
                     EKEEVKND"
     gene            complement(35243..35416)
                     /locus_tag="KPK67_000523"
     CDS             complement(35243..35416)
                     /locus_tag="KPK67_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HBG2457530.1"
                     /translation="MKLKDIIKLGEKYCYCPSCGNDKIGNNEGKLIVEEHTYYRECKC
                     GFNILIDDRKDEI"
     gene            complement(35413..35571)
                     /locus_tag="KPK67_000524"
     CDS             complement(35413..35571)
                     /locus_tag="KPK67_000524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457531.1"
                     /translation="MKIQMQLTKDKESFKVCVNSEKEELEKLFYEFVSQMLTRKRKSK
                     TVQGDIEK"
     gene            complement(35546..36004)
                     /locus_tag="KPK67_000525"
     CDS             complement(35546..36004)
                     /locus_tag="KPK67_000525"
                     /inference="COORDINATES: protein motif:HMM:NF021182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457532.1"
                     /translation="MSLIEYRGYDFENKKWIYSKTIMWSNLVDCLLMLKEDFKWRKVC
                     DVGICSEEWDRNNQEIYEGDILKEYYNPRDTSQYGVVKRDFRSIKLYLEWHYLKKFEG
                     EWIEIINKTEIYHSRDYKVVGNEYENLEEVRKEFLECKEKLENENTNAVN"
     gene            complement(36001..36138)
                     /locus_tag="KPK67_000526"
     CDS             complement(36001..36138)
                     /locus_tag="KPK67_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457533.1"
                     /translation="MVLANVEQVIKLAEKIINKKKCSVNKAIDIAIKILSRYEYEGMI
                     K"
     gene            complement(36107..36454)
                     /locus_tag="KPK67_000527"
     CDS             complement(36107..36454)
                     /locus_tag="KPK67_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457534.1"
                     /translation="MAKVWMDAGTFLETKDIGDIECNLREMINKNKQKNLIGRINSKG
                     QGRKGKKVECTNIITGERKVFDNAVQASKCLYFTELYVGYLARTGKTTKNGWKARYIK
                     EASNGTSKCGTSN"
     gene            complement(36469..36726)
                     /locus_tag="KPK67_000528"
     CDS             complement(36469..36726)
                     /locus_tag="KPK67_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457535.1"
                     /translation="MIIIRSQDKTDLVKADWINVDKEYVYAMFGEINNFKEIGKYEDE
                     KRAMQVLDNIQRFIEGGTRTDSIDSYKVRSYRNRIFQMPVK"
     gene            complement(36744..36965)
                     /locus_tag="KPK67_000529"
     CDS             complement(36744..36965)
                     /locus_tag="KPK67_000529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457536.1"
                     /translation="MRFEIGKTYKFDKEKFMEINGVEQYKEYKELWIDDIEGVEFTVE
                     KTFDDGYVCYPNEFRFNFGVISEWCVEVK"
     gene            complement(36955..37131)
                     /locus_tag="KPK67_000530"
     CDS             complement(36955..37131)
                     /locus_tag="KPK67_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457537.1"
                     /translation="MSRTAICSVCGEFLVDSYGDEFTNAKVVDLYGREFVIVICKGCL
                     EELLRKCEEQQYEI"
     gene            complement(37157..37573)
                     /gene="ssb"
                     /locus_tag="KPK67_000531"
     CDS             complement(37157..37573)
                     /gene="ssb"
                     /locus_tag="KPK67_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2457538.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSIAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLTKGKQIYAFGELNIDNYKDENGCWKSITKVNVNALEL
                     LSSKEDSNNHEKQQEYIPPGLDPQGFQAIDDDDIPF"
     gene            complement(37590..37763)
                     /locus_tag="KPK67_000532"
     CDS             complement(37590..37763)
                     /locus_tag="KPK67_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBG2457539.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKIAREKQNSKTTY
                     TYFVEYTEEHEEK"
     gene            complement(37823..38752)
                     /locus_tag="KPK67_000533"
     CDS             complement(37823..38752)
                     /locus_tag="KPK67_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2457540.1"
                     /translation="MKEDKSVLEKLNILSGGYGLMPRIIARDRWLTVGARMLYSYLTS
                     FAGNDGTCFPSRDLICYELDISKDTFTKYKKELEMSGYIRIHKNKSKQGKMQNNIYEI
                     VFDRTYIDECISRRGLKEEKKKKKPCPKKQDTEPYPKNVDMEPCPTFPDTTQPDTENM
                     DTNSNSINSNSINNMYIGKQPVDNSLKEFKKLYEENIGVIYPVTAEWLVEISNEVDIR
                     VFKRAIEICAEKMNMNLAYLKGILKKWKDANITTYEQLESYRLQQENKKTKKVVNNQL
                     SKNKFANFNQTFTQYDEKELDEIIKKSQKEKFK"
     gene            complement(38767..39669)
                     /gene="bet"
                     /locus_tag="KPK67_000534"
     CDS             complement(38767..39669)
                     /gene="bet"
                     /locus_tag="KPK67_000534"
                     /inference="COORDINATES: protein motif:HMM:NF015776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HBG2457541.1"
                     /translation="MNNKALELATCTLESGQILDYMTVKNYLVSGNGNVTDQEVLMFI
                     ELCKAQKLNPFIKEAYLIKFGNSPANIVVGKDVFVKRANKNPNFEGMKAGIVTVNKNG
                     EVFEREGSLKLPQEELIGGWCEVSVRGMKFPIKSVVSLEEYSKSQATWKQMPCVMIRK
                     CAIVTALREAFPEDLQGLYDSAEIKTVPDKLPQKPIEIGKASPSQKQGILKLASMKGL
                     YDYENKKDTSKLEEFCESNGFDLKELKFEEVDELIELLSEYEPKDDFIDAEFEELKED
                     EVEVKEEAENIDCQISMEEAINVD"
     gene            complement(39671..>40037)
                     /locus_tag="KPK67_000535"
     CDS             complement(39671..>40037)
                     /locus_tag="KPK67_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBG2457542.1"
                     /translation="IINIDSDINSIDSEIKRLQELKRVKKNTLDRLKSNIKDCMELLG
                     TKKVETVLGNISIRKSAGSLVIEDEEKIPSIYKTVEQIVKVDKNTIKDFIKKGHEVEG
                     CRIEYSTTLTTPKVKKEVN"
BASE COUNT        11543 a         7703 c         4198 g        16593 t
ORIGIN      
        1 tagcagaaga tgcactagga gtattgtcag taacataaaa gtattctttt cgacttccac
       61 ataatatctc tatttttgct gttgcataat cagcacgcca tttagacgct acaagagacc
      121 ctttgaatct taatgtacct tttatactag atacttcatt gtaaacaatg ctaggagtgt
      181 ttaaagttgt agcttgaatt ctatttttta aagaaaaaat ttctattgat tgaaaaatcc
      241 agttaacttt ttcaatcaaa ttagtaaatg tttcagatga tgttgctgaa acatttttag
      301 aattaattgt ctccactaat acatttttta atgtttctat ttttgtttta gttgtaccaa
      361 atttatctgt tccaataaaa ggactaccca atgcactaga tatattattt ttaccagttt
      421 gaaaatcagt ttgtacattt tctaatgctg ccataagttc ccctaaacta gcattttcag
      481 ttaatttttc tgtcatattc tcacctcgct tatatcatat ctatcaaatt atttgctatt
      541 gtaatgcctt tagccctctg cccatttact tctgtttcta aattattctg tttttctgat
      601 aatgtattat aattttcagc taactctttt aatgctcctt ctacattatc actttcaaat
      661 aagttgtctg tatcttttat acttgttttc tctgctgttg tttctatact atctacacta
      721 gtttttacct cattcaatgc actaactata tttgttttat ctgttgtagt aagttgtgtt
      781 gtatccccta ttttctcgtc aatttctgtt ttagcagttt ctatgttgct ttttaattct
      841 gttttagttg tatcaatttt agtgtctaag tcttgtatat cttttaaagt tgctaataca
      901 actgttgggt ctatttttaa atttatactt gctgtattag acacagctaa tatgatttta
      961 attaatattt cttttactgt tcccgaatct ggagcaggtt tataagtttc tggatagcta
     1021 gatattgcaa ggagttggtc tttagaatca aatactccta cctctctgat tgtaaagctt
     1081 ccagtatctc cagttatggt cttttgtata actacccaat taggattttt ttcatctgct
     1141 tgaacatgct ctaatgtact ttcccacacc acatttttaa gttctgtttg agtttctgtt
     1201 ggaatatatg aacttcctcc accatcccca acttttattt ttgcaaaatc aactctagtc
     1261 ccaagtgctg tagcatttgc tatagctgct ttgcctattt ctgtaagtaa tgtataatat
     1321 tgtgcttgtg ccaattatat cacctcctgt ttagggtata atgttaccct ttcagcactt
     1381 ttattgtttc cactagctag tattatttct ccaaaacatt ctatattttt aggtgtataa
     1441 gggtatatag taactgtctc ccccgtactg gtcattgctc ctgcataaag tccattttca
     1501 ttaaataaaa tcatttcaaa tttatgttca agatgtgcag gttttatttc ttctattttc
     1561 ttatctaatt ctaaaatagt gttataacta caattatttg ttataaaact aagtgtaaaa
     1621 ctaaatagat tactaaatac ttctacatca acattagtct ttgtataagc ctctcctata
     1681 gcttttataa cctctattgt agttgtaccc ttccccctca tttttgcttt tatattactt
     1741 cttctatctt ctatatttaa atcaaatcta tttttaatag ataaaatatt ttcccaataa
     1801 tctaagcccc atgtggctgt atctacaaag aactggtcaa aagtatcgtc ataagtttct
     1861 ctaagtgttt ctagttctat gtcataagct tcttgtattt ttctagtaat agaattgtta
     1921 taaaaacttg gtagcttatc aattaacttc attaaaccac ctcgctaaaa tttagagtag
     1981 ttacacttgg tactttgtct tcttcaaata ctatattttc agctttatta tttaataata
     2041 aattactaaa gtcatgtaaa ccttctatac ttgcaagtat tgcgcttact ttagtgtaaa
     2101 ttatttcttt attaacattt attaaataac tattaatact ttctaagaag ctttctttta
     2161 caaagtctat tgtgtatcct gcttctagct ttatagttgc acttatgctt acatcaagta
     2221 gtgaaggtgt tgcaactgtt aatgcaggac ctattggcat ttctatctca atatgttctt
     2281 tgcatctttc aattacttct gtgtcaacag cttgattatt ttctccaaag attaaaactt
     2341 ttaccgttcc tggaccatcc catctcggat agattttagc gttatatact ccttcaactt
     2401 ctaaagccca tgcttcatag tgtgccttat ttccacttgt agcttgattt ttttgtattt
     2461 tatagaatct ctcttttaat tcttcgtctg tttctatttc cgtcccacct ttaaaattta
     2521 aattattata aattttcgtt ataccgttta tttcgtcttg tagcttaaat tcagtgtttg
     2581 caggtatatt atatctaatt ccaatttcta aagcctgtac ggggcttgta ttttgttcga
     2641 tttctgaact aattactata tctttaatta ctacgaataa tagctcattg taagatacaa
     2701 ttgttccgtt ttggacaact gttccagctt ttccctcgaa taccacttct cctgtagctt
     2761 ctgttcctag ctttctatat actccaaatt catttacccg tttatcaaga aaatcatcaa
     2821 aattatcttc aataaaagct tttttatgaa gatatgaaag ttctatatag aattttgcga
     2881 gttctgtact gattggagat accatatcac ttaaaaaaga accctcaccc ttatagatat
     2941 ctaaatctat atttgataga gttctatttt taatgacatc ataagtttga ctactataca
     3001 ttaacttcca cctccccata aatcgtgttt atgactatat ccacgcttaa taaatcgtct
     3061 gtaaattttg tattcttgat attgacatct aatatatatg gattaactaa taaagcttct
     3121 tttatatacc tagatgcttc actttctgta agtcctttac tgtacttttg tcctattaat
     3181 tcagataatt ctgtcccata atcccaggaa taaatctcat gctcatatct atttgttttg
     3241 atgcatttat acacccaaac cttgatagct tcattgcctt caacaatctt aaaatctcca
     3301 ttttctaaaa taggttcatc tttttcaaaa ttccaagcca cttctctaaa taattctaat
     3361 tcttgtgttt tcggtatttc atagtctgct gggacaccta taaaaggaaa tattgtactc
     3421 attataagct caccagctta cttacaacag caaatttatc acctattttg aacattatta
     3481 ctgtgtctcc agattcaaat ttatctataa atggattttt tacttcatgt tgatgttctt
     3541 gatttgtttc tgtatcaaat gattctatct gtctatcaag catccaacta tctatcaaaa
     3601 tatcttcttt ttctaatatg atgttattta tctctatttt taaatctggt aatttacttt
     3661 taatttcccc aataaaaaaa gaaggttcat tgtaatactt tccttcttct cttattattc
     3721 ctataaattc atttatcgga ttagccatta tatcaccacc tttttataaa taccttctag
     3781 ctgttacata atttttagcg taataactac cacttaactt acttactttt acaatatcac
     3841 cagtatgtgg agaatgaata aattcgccac ttccaataaa catgccaacg tggtctattg
     3901 ctccatttgc tcctttacta gaaaagaaaa ctaaatctcc tggttgtaaa cttcctttac
     3961 ttactgcttt gcctgtcttt ccttggtctc ttgatactct aggaatgttt atacctatct
     4021 ttttgtaaca ccattgagtg aacccactgc aatcaaaagt atttggacca gtagctcccc
     4081 aaacatactt acagcctagc ttgctttttg ctatacttat caattcttta gcttttcctg
     4141 ttgcatttgt atagcctgtg ccatctccaa ttattattgt tccttttctt cttccaaaat
     4201 cattacattc tttttcacta gacattaata tatctattct atacactcca ttttttaagc
     4261 caatcgctcc tcctctgtct gttactgtat aagttttacc atcaatttta gttccaggac
     4321 attttgcttg aatttttgtt ttaaatttaa gtttactagg tgcagcacaa gtattgtttg
     4381 aaggtacaag tcttttacca tccatagctt gataataacc accctccata gggttattag
     4441 acggataata agctgtaaat tctgctttta cttctcttcc gtttagcgta ccttcaccac
     4501 tactaaaatc tgaactttcc tctttttgtt catcttgtcc tgctgtcttt tcatccatga
     4561 tattttgaaa attcaagtca aggtcaattt cataatttcc gctactgtcc cagttatgtt
     4621 tatctgtatc tatataaaat aacccaacta aaccagtata agagtcttta acttttacac
     4681 ctctaccagt tatacaactt acgtcaccat atccttttaa actgcaagtc tgctctattc
     4741 ccttaaactc gctatctata tctgctgttt gattttcttg ttgttgtata actttttgca
     4801 ttattactcc aacatcctta aaaatctcat cgttgacctt ttcactaatt ttattcccat
     4861 attggtctac aactaagacc ttgtttttta cattctccat actttccgaa aagcttgtat
     4921 ttataagatt ggaaccttct tcaaacataa catttagcgt aacaattcct ttttcaatga
     4981 cattaaactt gtctaaattg gcttctatca tatacttttt cttagttgtc ttactagctt
     5041 ctgtatatgc actcattata gtgtcataac cagttgcacc tatatacatt tttgtatgtt
     5101 ttactccagt cttaggtatg tttccgactg caagtttatt gtcattaaat acttgttttg
     5161 caatgtcttc aactaatttg tctttgaagt tatatgatac ctcgctttga gtaagcaaaa
     5221 aacccatatc tttagataca aaactaatac tattgttgct agaatcttta gacctattaa
     5281 ttatcattcc tctatagatt tctttatcat ctacataaaa acaaactgta ctagctatag
     5341 gtatgtctat ctgtctaaaa ttaatatcag aagcagattg tactatagaa aattctaatg
     5401 ttctggaagg tgacttataa tcaccactcc aagttacttt ctcaactata tcagttatat
     5461 catagatagc tccatttttt atatggacct gtaattttat attattaatc taaatcacct
     5521 cacttgctct acacatataa aaacaccacc aaaaatgata aaatatatca tgaaaggagg
     5581 tgtttttatg tatgacttca aacctgtaac aggttattgt ccttttttag aaaaggatat
     5641 tactgttact tgtaaatttc tcttagtaca taaacctaga gagcttaatc ctggtaaaaa
     5701 attcatggga cataattgtt cacaagaaaa atgtacttat gatggtaaat cctgtccttt
     5761 ggctaatcta ggaagagaaa cttattaatt agtctataaa acctgcacct ggacacattc
     5821 ttactcctgt aatttttgat aattttatga atgtgtccca gtgtgcataa ccatatccac
     5881 ttttaaaaca ttctctttca tacttgcaag tttggcaagg gtctaatata tcatgttctt
     5941 ctctaaaaca tctctttata acgtttattt gaatatgttt tgccatacaa tataagtctt
     6001 tttctgtcaa atcatctttc gttagaccat agttttctgg tattattctt actttctgca
     6061 actctatcac ctcacttaag gaattattaa tatccagcca tcttttatta ggtcaggatt
     6121 tttaattatt tttttattag catcataaat tttcttccac aaatctccat tgccataata
     6181 tttttttgct aaactccaaa ggctgtcacc tttgcctacc ttatgtgttt tttgcttagt
     6241 atcaaatcct tttgttaggg gaacatcttt tacagaagat aacttttcat cattattagt
     6301 atttacttta ggtatctgta ttcttttata ctcttttaaa tttaaggtga aatatacatc
     6361 tcctgtgcag tctttttctt catatttaaa actatctatt attacttcca tatttatatt
     6421 tgtttctgta actgtaaacc ttaatataaa gccttcattc atccattttt caattttatt
     6481 tacacagtca taaggcgagg gaaaacctgt ataatcacaa aaagtatagt catttttggg
     6541 aaagaaacta gatatttctg tagttcttaa gcctacacct ccaaatactg caatttcccc
     6601 tgctttcaat acatttgagg tatttactat tgctttactg tttatctcaa aagagcttgg
     6661 aaaaacagga aatctaaagg tgtcatttgc ttgtctaatc cacatttcca actacatcac
     6721 ctctatatct attctaaaga tattaaaaac ttagcttcat caattatagt tttttcaaaa
     6781 gctttatcca aattttcata cctagcaata tcttcttcat tgtttgaata ctcatgtata
     6841 tcttcaatta aattttcttt tgcttgtatc aaaattttta acctcattga acaagcaata
     6901 aattctatat ctttattcat tcaatcacct tcttttttca cacaaaaagt acttgctatt
     6961 aaaacaagta ctttgcatat tttttctaat ttgtggtata ataaaagcaa gaagaactac
     7021 aatctattta agactagagt ggagttcgta aattagtgtt tctttttgaa cttaatcttt
     7081 agttcaaaac taaagtcact ctggccggag tggcttttta cttttttgat acatatacaa
     7141 actatgtaac cgattagact agctgttaaa ctagctaata cgctaatcaa aaaattatcc
     7201 atacatatac acctcccttc tatacgttgg gaggataatc ttttgtatga actccactct
     7261 ataaattgta gattacatct tcttgctaca attattataa catataattc ttacatattt
     7321 tacctattta ttacttttta cattataacc accttctttc aattaaaaaa cacctaccta
     7381 agtaagtgtt tttcatttat ataattaatt tcttatttat actttgcttg tttacaatat
     7441 atatctacta tttgtttgaa aacataaaaa tcatgttcat cttcttgttt tagagttttt
     7501 aggtaagaaa taagatattc tttttcatca ttacatacat tatttagtaa attatctccc
     7561 attacattgc ctccttatat acaacatgga aattcttctt ctcaccttgt atcttagcag
     7621 gtctattatt ttcctctatc caagttctaa ccttatctat tacactcttt gtatatttat
     7681 ttacagtacc agtccaactt ccattagttt cccaaactcc ttttacttca ttttcttcta
     7741 aatcaatctt tttaataatc tcacaaacag caagttgtgc tggtttatta ctcttagaat
     7801 atattttcaa tttagacgct atttgttttg tatcaaaata atgttcttct tcatctatct
     7861 cgattggtaa atcaatgcct gcctttttat ataatgtttt agctgttaat agttttgatt
     7921 tattgtcaaa gcctgctcca tccaatagtt cttttaacat agacgtactg ttataagcca
     7981 gttgtaattt ttcaatctca cttgcttttt ctctcaactt atctgggtta gcattgttag
     8041 ttatgtatgc accagtttgt cgaatggctg gaagtacttc atcacttatc cagtcttgga
     8101 acttctctgc ttcttctttc tgagatttaa atattaactt gtatactcca ctctctgtta
     8161 agaacttttc acctgcatta tttagttttc ggatgtccgt atctaggaca gctgaatttt
     8221 ttaataatat agcttgagta tcattcattt tagacaaatg atttcttatt gcactatcac
     8281 ttagctctaa acatcttcca caatcgtatg gattaaataa aacttgccca ttatattcaa
     8341 atatttcaat ctcttttcct tcaaaattca ttatttcatt catagtaaaa tcctccttga
     8401 aattttattg taagaagtac cttactatga taaaatattt catagaaggt aacttctggt
     8461 gtaagtagtc cattttgctt tggtcggcgg ttgggctact ttttatttgt cttttagtag
     8521 ttttatacct cttctaactc cttcggcttt gtctatatct tctttttcac agtatttctc
     8581 tagaatattt ttagattctt catccaatct tatagttatt ctactgcctt taggattatc
     8641 tgtaggtcta cctatttttt tacttggcat atcttcacct cacttttgtc tgccttaatt
     8701 atattatata gtttcggcag acaaaagtca atcatatatc caattttttc taattatttt
     8761 actcaaccga ccaatttgag taaatttgag caaaataaaa acacctacat atttgtaagt
     8821 gctttctttt tctatttagt ttttctccac atagttaata taaaactgat gacacaagga
     8881 caacagaggg gattagatta ttcatttaca taccacatag ttaatataaa accccaaaat
     8941 aaacttagca ttttcaatac ttacatatat acagctctct taaatttgca gtgagcgacc
     9001 agtagtgcaa ttgataacac ttatcataca ttcttaacac cttatatttc aactattaag
     9061 tcctactttg tcgcaaatat cgctcactgc aaaacttcta tatttttatt atatcataaa
     9121 aataaagttt cttaaatata acaatgatac aattgactga aataaaagtg cttacttatt
     9181 tataatatat ttctaacatt atagattcaa taattctttt ttcttagtat taaattcttc
     9241 ttctgttatt gctcccatgt ctaataactc ttttaatgtt tttattttct ctagtgggtc
     9301 aataacatta tttttatcag ctatattatt agcatcttca actttattgt tttttctata
     9361 ttcaactaat tttttataaa tagttgcaca tacattgtca tttttcttaa aagataatat
     9421 aaaatcttta cattctacta ttaagtattc ctttttatct actgtctttt ttttcatagc
     9481 aagagcaaat ggcccaaaca aagctattct agttgctgta tatcttctaa taacttcctc
     9541 ttctttttca atagaaacac tttttatttc tgaataagaa acttcaaaaa catcaatcat
     9601 tttatcattt gattttttcc cagatttttt aaataatatt ttattatctt taactacaac
     9661 aaatagaaac tcttctttat ttaaaagtgg atgaccacct atatattcta acattaccat
     9721 ttcgctatta ctgaagtttc cttcttcatc tttactattt tccatacatt ctaaaatatc
     9781 atttttagtc aattttttta ttggcttacc tgatgcaact atctcaactc tacatttttc
     9841 gagacaattt ttgcacaagt acccatctaa aacttttaaa gcatctgtat ccgctccaca
     9901 aattatacat ggttctttta ctattttttc ttttctacta aataatccca tatttatccc
     9961 cctaaaacta atatatacta cgattatagc atatattgtt acattttagg gacgtatatt
    10021 tctatacacc acctgctaaa tttaatttta tttctctaac caacatctta gttaatttag
    10081 tcatatcagc ttcttcttta actataacac caccccaatt tatatttata gcaatattat
    10141 tattttcttt attgtttgta gttttactat cagttttatt aatagtattt tgctgagttc
    10201 tttgtgttat agcttctttg tacttacttg catagttact ttctttaatt tcttttggat
    10261 tatacattct ctgagttaaa ttactaatta cgttattaat attattcttt aagtttggta
    10321 actctttctt tatacctacc caaattcctt ttggtagatt tattcctatt attttttcca
    10381 ttataacaga tggagaatgt actccaaatc catttttaaa tccatctatt acgccttttg
    10441 caaagtcatt tactttccct tttaaccagc cacctgctcc tgcgatacca ttccatagtc
    10501 ctttgacaat gttttttcct atatccatca cctttttagg taaagatttt aatgtatcca
    10561 ctactgtatt tattaatttt ttagctcctt ctttaccctt tgttgccatt tgtcttcccc
    10621 aagtaacaac tttttgaact gtatttgtaa gccaagtcca aatacgatta ggtaaagtag
    10681 taaaaattgt tattatattg tttataatca ttctggagta aatttgagca gcagttaaca
    10741 ttgcgatacc ccattgacct attttttgaa ctgtacttac taaccaaacc caaattttat
    10801 taggaagctg cgcaaaaaat gtaacgacat tgtttatcca aatcggaaca tttgtaacta
    10861 agtaagtcca tacactaatg ccccaagata ttatttttcc taatgcaaaa cctagcccat
    10921 aaccaatttt tgcaggcaat tgtccaaacc atactccaat attatttatc catgttggaa
    10981 ttgtttgagt aaagaaagct acaatagcat tccagccatt tataaatgct tgttttatat
    11041 tattccacat ttctccaaac cattgtcctg catttgaaaa acttgtacat agactttccc
    11101 aagcttgagg aattgtttgt gtaaagaaat tacatatgcc accccaaacc tttgttgctg
    11161 tttcttttat attattccaa gcatttataa caaaatctct aaatccttta tttgtgttcc
    11221 ataggactaa aaaacctgct acgagagtag ttactgcaac tattacaact ccaatcgggc
    11281 cgcctagcaa caaggaggct ttaccaaaaa gtgctgccgc tgccgctcct actttcattt
    11341 gtttggttaa aacggatact ataatttgaa aagtcgtcat gcctagtgcg gctgtcgttc
    11401 ctgcactatt gctaaaagct aatgctatac tataagcaac agctgctcgt ttagccgcct
    11461 tccaagcatt gactaaagtt actattccct ttactacact agtcataata gaagcaactt
    11521 taatgctcac aagtatactt gcaatagtag gtccattttt cagaacccaa ctaagactct
    11581 taatcatagt tggcaatgcc tttgtcgcaa tttttataat tgtagatgtt aaatccccga
    11641 aagattgtgc tatttgagaa accccttctt ttaacgaacc atttgaaaat tgattattca
    11701 aatcttgtaa acattttatc cctgtttcag ctgcattttt caaaggttct tttagtttac
    11761 tatatatctc tatacctaga ttcttaaaac tttcttttaa tattcctatt tttgattcta
    11821 atgtattaga cattttagca tacgcacttc caacgacatc agcttgtgta gacatagctt
    11881 ttaaattatt agtaaattgc tctgaatttt gaccactaag tgcgagtgct gcttttcctg
    11941 catctattcc tccaaacata tcaatcaagc tttttccact ttttttagca ctagcatcca
    12001 ttaaatctag taccgtgtta agaggtactc ctgcttgcat aagttcttta aatgatttcc
    12061 cagcatattt agttcctttt gttgcactta ataaagattt attggcaagt gtaccattcc
    12121 ttccaaggtc agcaattaat ccgtttaatt gtgtaactgc ttctgatgtt ggtgttcctt
    12181 gtgctgtcat actagcgagt gctgctccta cttgctcaaa ggatactccc atagctgaag
    12241 aagttgtcgt tacgcccgca agtgcacttc ctagctcatt tacggtagta atacctttat
    12301 tttgagtctg catcataact ttgtgtactc tatctgtttc tgatacatcc atcttgtaag
    12361 catttaatac ttttgcagtt gctgttaaag ctgtgtctat atctgtaaat cctgctttag
    12421 ataatttagc gtttttagtc ataaaatcca ttgctgaccc catatctttt gtgacaggaa
    12481 taccagaaga tagagcgttg tataaacttt ctcctataga agatgctgca attcctgtat
    12541 tcttggataa ctctaatact ttattattca agttttctgt atctacttta gtgtctccaa
    12601 acatagtgct tgtttttgtc attgcagttt caaattctgc ccctgcctta gatgcagaaa
    12661 caagtcctgc acctatccca gtcacaatcc ctgctcctgc tattttagca gcattactga
    12721 cctttctaaa ttgttcttgt aatctttctt gtgcttgtga tgtagaattt gtttggtttt
    12781 gaagagctct agtagcatct ctaacttgtc ttaaagttct actagcttca tctcgcattc
    12841 ttataacagc ttctaaagct cgactccctg ctgacatact actacaaccc ccttcctaga
    12901 ctctttatct cttctatttt ttcatccatt tcttgataca taaaagctct aattacatgc
    12961 ttttcaccat atttaagctt aaaaaaatca gatggcatca tattatggta tctaaacatt
    13021 agatacatta aattgatttc tccatccgat ttgattagtt ttttattttt tcttctattt
    13081 ccttatcttt tttattatca tctctatacc cattcaattc acaaatttta tcatataatt
    13141 catttatttc ccctgcaagt aacattcttc ttaaaagttc ttttggtgtt ggagattgga
    13201 aatgttttaa taatttatca tctttaaata gattacatga tgctagtatt acattagatt
    13261 tcattttaaa attgtctaaa tcctttaaag aacctttttc tatttttaaa ccactagttt
    13321 gaagctcgtc aaaaaactct ggctctactg ctttacattc aaaacttaca tctattccta
    13381 atttcttgca aaacattgta tgtgttatgg aaggcatttt taccttatca gcatctaaac
    13441 ttaacaacaa atctacaaca ttttttacct ctgtcacttc ttttttatct atcatttctt
    13501 tttctaattt attttcactc atattttttc tccttattta attattttta tattgtttgt
    13561 attggattgt aatcctcaaa agtgaatgaa acttcttttt ctccttcttt tccgttttcc
    13621 cagtctataa tagtaagccc atcaaaacta acaccagtta ttgcaattct ctctgtccca
    13681 aaagaatcag ggtctgataa tttacttgtt attgtaaatt taggttccct accttcttta
    13741 ataatttgcg taacataatc taatattttt gaatctacat gatatagtgt tagagaacct
    13801 tctgcactag caccaattat cttttgacct tttattattt ttcgaggtct aactatttcg
    13861 gctttgtcaa attttatttc agctttcata gctttacatt cagctatttg ttcattatta
    13921 atccaaacct ctccatatgt tccatttata acatttctag gctcataact tgtcgccatt
    13981 ttttcaccta ctttctacat ctacatttga acgcttatat ttatatcttc catagcgtca
    14041 actagtttta attttattaa ataaaatcca cttgaatccg tattagcttc ttttatttca
    14101 tcttctttca tcttacttgt gtctaccttt ttaccttcta agtattcttt ttgcttttct
    14161 atatcaatat caacggaaaa atttgaatct attagttctt gtttagctaa ttcatctaaa
    14221 tatgcttgta ttgcaattat aaacaaacac ttattatcat aaatattcgg aacttttcct
    14281 atataacttt ttacataaat atcttttata tctttgctta ttaagtcttt agtatcaaca
    14341 agcttaatct tctgaaatat ctctcctttt tcttgtgtta gagttgtaag agaatttaca
    14401 cctctagcaa tccttatagc tcctgacaat cttcttagta ttaattctcc cgattgtact
    14461 ttagcgtctg cactagcttt gtctatcttt gtaatagatt caacttcatt aagaggagca
    14521 taagtaacag attgtgtatt tggtgtagat gctataagag aagcaatacg tggcgtgtat
    14581 ttttctgctg ttatttcttc accatcaatt gttacatttt cagtaaagtt aacaactgct
    14641 tcattatcag ccttaatatt tgctaatacc actttggctt ctgtgctttc ttcctctctt
    14701 atcttcttaa tccaagttac aatttttgtt ttttctgctt ctatagcctc tggcatacaa
    14761 agataattaa attctacaga ttctaattct tctagtatat cctcaacttt tccatcttct
    14821 gtgaaagtgc ttataataac tttacttggt tttagtatct tctcgttatc agtagcccct
    14881 ataaaagcgt atttaacata ttttttatta tcatcactta atgaagttgg tatatcatct
    14941 tcgcttgtaa gctctttatg catctttgtt gtatctttca atataattac tactattcca
    15001 gtttgggaac gctgtataaa acttgtagcc aactctttaa actctatatt tatgttaact
    15061 aatccagcca tttaatcact ccttccttca aatctcatat ttagttcttc catcaactca
    15121 tatttttctt cctcaaaata aatttgctca tgataattta tagatataag aaattgaaca
    15181 taatttccta tttcatcttt ttctatactt ccacttttct tactaaaagt taaatatctg
    15241 tcttttactt ttatatttct agtgaatatg ttctctaatt tatttagaat gtcgagtaaa
    15301 tctgttttct tttttcccca tttaggcaaa tattttatat caactaagaa gctttttata
    15361 tcagtttttt tagtcgctgt ctgtaccatt tctggcaata tctgcacaaa aaaacaagac
    15421 tttttattgt cttgtatgtt atattctcct acaactatag tttctttaaa attattatct
    15481 atagttttag taaatgaatc tagtatttca ttataactta gcaacacatc acctctaatt
    15541 ccaaaaatct attattattt gatttagttc attgtcgatt atgctactca tttcatcaac
    15601 actccttgcc aacataaaca cacctggcac aaaacttatt ccatcaggtt taggtctgta
    15661 gttttcgcta gttcctgttc cttgtctagt tctatgtcca tattctagat gataaatata
    15721 ctcaacattg tttgatacca cactatcaaa taaactaagc tctttatact tccagctctt
    15781 tctagctgtt cctccatcta tttcagcaac tggtgtctta ggctttactt ttctaagtag
    15841 ctggttgcct acattatttt tagctcttct tagattctta gccatttcct tttcttctct
    15901 ttcaaaatct cttattaaat catctagact attaaactct atcattagta tctttccttc
    15961 aaagttagat tagtttgtag gtgacttagg tgagggaaag gtttagatgc aataaatgtt
    16021 tctacatttc cgctttctaa agttatatca agtatatctc caactctcaa atccacttcg
    16081 ggtctgcaat ataactcaaa ggctgaaatg gaacttgtca tatctgtatc tatgatgtta
    16141 ggtatatttc cattcagacc acaaggcaca tcttctgtaa ttacaacatt ctcgttaaaa
    16201 tccgtaactc ctgtctcctc atttttaata cttacacatc ttcttattgt cattttacaa
    16261 aagtatgtca gtgctagtat atctgcttct gtcattttac cacctaactt ttctaaaatt
    16321 attcaaaata tttttatcct tttgagataa ttttatttct attaattcat ctgtcgtttt
    16381 agctgttgca acattgtaag taacagaata accaccccta gaaactgaac taattttgcc
    16441 gttatcagca ctattattac ttaataaggt ctcttttaat ttaacaataa ctttatcttc
    16501 tataatattt tcaagagtat cattaagcgt ttctatattg cagtatgcaa gaactagagt
    16561 agtgtatttt ttaatgtaca actctattaa actatcatga gtatcatctt ttagatttaa
    16621 aattaatttt atattatcta gcatttaatc acctcgaata aaaatagaga aactaaatat
    16681 tatttaattt ctctattaat tcatcttttt tcagctttga ataaccttct atgctatttt
    16741 cttttgctag gtttttcagt tcttcaatgc ctaaattttc tatattaaca tttttcttat
    16801 cttctaataa cttaaatcca ttatacatta acttttcttt ttcagatgta tcattaacta
    16861 ttctttctat gttatctctt attaatctat acataattta tctcctttta gactgttggt
    16921 ttagcatcct taaagttagc atatactaaa ttagaattta gattagtaac ccataactcg
    16981 tgatatcttc tataatccat agcccaagaa ttaccacctt gataagttaa agggtcaaat
    17041 attctcattt ggtcttgctt tgtaacggcg ataggtacat ctcttcctgc tattataaaa
    17101 ttacaatcta gtgctttgct accttttaca tatccacctt gcttttgacc ttctgatttt
    17161 ccatcatata atgttattga tgaatacatt ctattctgag gagtagctat aagtggacaa
    17221 aaatctattg ttggtacttt tgtagttatt ccacctttgc taaaatcaat agccattaat
    17281 ttttctaatg ttttttcctc aactgctgtt aatgtatcat aagttaaatg aattacaagt
    17341 tcaccagtat atcccgtttc ccttattgtt gttattcctt tttttatttt acttataaca
    17401 gtatctttgt gtggtgtata tccatattct acattagtat cattttctac attcatagct
    17461 acagtagcta gtttacttaa tcgataagca tcaacttctg gaattacttt tgtcctttga
    17521 aattttccca tgatttcact tgcagttaac gtgaaattag tttcatcaac gtcatttggg
    17581 tctattgtaa actttcttcc cctatcctgt gtcattgttt ttgtttcata accaaacttt
    17641 atatctccat ttgcatatcc atctgatgaa cctctgttat aatctcctaa tccatccact
    17701 tctaaagaag gtatttttat ttctttccct ccattataga ttgcttttcc tgcgttagca
    17761 tccatccatc ctgtcaaaag agtttgaatt gctgaatcat caagggcttt ttgtatagtt
    17821 gacgcatatt gtattacatt tgccatttta acatctcctt tattttaatt atatttgtcc
    17881 gtttactgct gcttttattt gttctgctgt aatttctgat gaagcttctc cactaccacc
    17941 tggtacatat ttgtaaaaag actgtgcatt tgtttgttta gtagtttcag tagtatcgga
    18001 ttcaaacaaa tctttgtagc tttcttgtaa cccttttaat tgttcctcta tccctactat
    18061 cttgccatct tcacctatag ttattttttc taagtcaaac ttatttgtta gtaagtcagt
    18121 gtgtttagct ttattagtta ataaagcttt ctcaacagca cttaacttcc ttgttttcaa
    18181 actttcgttt tgtattttct caacttctag tttatgattc tcttctatct ttttaatttc
    18241 tgtttgatgc tctgttttaa gcttttctac atcttcttga gtcattttac tattaaagct
    18301 ttttatagtc ttatcagctt cttttaattg ctcattcact ttgttgaaag tttctttagg
    18361 tactgcatgc ttgggaaatt cagtattaac attttttaat atttcttcaa tatcaagctt
    18421 attttcttcg attttaatac cttctaatat ttttcttaac cattccattt aatcatcatc
    18481 ccttcttatt ttctctatag atttttatag ttgccctcca actgcgagag ttactttgtt
    18541 ctttatgctc tacaactttt ttaaaaagag caaaataaaa aagccctatc taggacttac
    18601 taaaaccaat attattattc tcattacaat tatttattga tatagcttct atttgtgata
    18661 aatctattat tgttgttcca tcttcatcta aatacccttt caaataccta caatctgagt
    18721 cagcttccat aaaatcttgc attaacttat cagcaacctc ttcatctact attccagata
    18781 cacaatttcc acttttaaac caaattacat actctttcaa taaaataagc ctcctttcat
    18841 ttttgcataa taaaaaaact ttaaacaaat tattcataaa taatacagct tattagcgca
    18901 gctataagat atattgcttt tcctatcaaa tacccactta taactcctac taagccataa
    18961 actaatctaa cgcaagtaaa agtattatta tctaccatgt tggctatacc taaaacatga
    19021 gccataatta tgttgatgcc tataaaaaca cctaaaacta taccaataac tgtaataaca
    19081 aaagctataa tatttctaga acttatttta cttggcttca taatatatct ctttttaaac
    19141 atatttctat cccccaaaaa cttattcaat actttctctt agacgttcct ctaattgttt
    19201 tacaatctta tctatatcaa ctttattttc ttttgcaaat atcctattta attttttctc
    19261 tagtctgtct actgcttcat cgagtgtttc aatttctttt gtagtatttt ctatattttt
    19321 atcaaagtct tttgtatcta attttaactt agctgaaaat tctaagctat ttttcttaac
    19381 cttttcttta tcaatgtatc tatgcccttg ttctaaattt tcagcatagt ttgttaaatt
    19441 ttcaatagtt gcagtatatt caactttctc ataagtcatt tcaaatatat cagctttgca
    19501 aggatatatt tctccttttg caccttgtat aatgtaatcc tctatgttaa cttgatatat
    19561 tagcccttca agagttctta tgaacatttt agtatgttcc ttatcaatag agtgttcaac
    19621 ccaagcattg ccacgttcaa tctcttttcg tatccatatt ggtggttcta catctatatc
    19681 aaatttaaaa gcttccactt caactgcttt ctttacaaat ctagccatat tatttattcc
    19741 tccttaaatt tttacacaat aaaagcactt actatttaaa cttaacaagt gctccctata
    19801 caacttctat attttttatt acatattcct tgtaccattc attataagtc aagtttgctg
    19861 gaacttcata ggttttacct tttggcagtc gtgccgttct catttctcct tcttcatgtg
    19921 ggaaataagg tatagttgta cacctgcacc ttggatgaag tggaggataa ttctctcctt
    19981 cttttgcttc tttaactaaa tatactttca tatctacatt tctacatctt ttacaagtat
    20041 tatcctgcaa tgttgctaag aattgatatt tatctactcc ttgactttca tatgcttttt
    20101 tatcagcttc tgacataaaa tgagaatgtt ccgtttgtac taatcttatt gcattttcat
    20161 aactagagtc catcttttcc gatactctat tagcaatctt tttcaaactt tctcctctta
    20221 taatcatttg agtaatttct tccttgataa cttcacttaa taaatctctg tttttccata
    20281 tcctttgaga aaagttttta ccactccaag gatatgtaat tatgtccttt aaagcttctc
    20341 tatcaagcct agcgaagctt actccaacac caacatgttt ttgagtttca tatatactct
    20401 tatagtaact atctttcaca gattcctcta atagcttttc tactctatta ttttgaatgt
    20461 caaatgtatt atatatctct ttagatattt gataaaacaa ttcttctaat cgatttatcc
    20521 tactcttcat agctaatgta ttaagttcta atagtaatct ttcatcacca gtttgttcta
    20581 ttaatttaat atattgctta atgtccatac gccatgtttt aaactcattg ttagttaaat
    20641 ttttttgtgt ttctgcatat gttagtttat tctgtttagc gtatttataa aataaattag
    20701 caatttcttt ctctatattt ttcattgcga ttttatattg tttatctagt tcttttaata
    20761 cctctttttc atcttttaat cttgcattta atctttgttt ttctctttct ttccagtact
    20821 caatattatt attcaaataa cttcacctct attcagtaaa gttattataa tcatcagata
    20881 cttttgaagc ttgtattttc ttttcttcta aataaagtct ctcagcttcc tcggggtcgt
    20941 cgacccaagg atgatgcctt aaaataattt tagttggtat tatgccaact gacttggttg
    21001 ctatatctgc atcctccaaa tcatttgaca tcatattgcg tgtatatgtt tgttgtatct
    21061 ttttatagtc tgtaactcct aaaaaatata gtatagcctt aattaactta tcaaaagagg
    21121 ttctaaattc ggtttcaagg agtccagact ttaattctag ctttctataa aagaatttaa
    21181 gtgccacctt aattgttatc gtaaaggctc tttatcctct acctcttata gtttcctata
    21241 agttcagact atctcataac ctacaacttt acttgtttag gcttcggatt tcgtggatat
    21301 ttctgcatat aaaaaagacc tttcggtctt aattacttag cttactttat ctagtcgtta
    21361 aaccttccta tgatttctca ctaggattgg taattgattg tctttttttg cgtttgtaat
    21421 ttacaataga gttctcgcaa atatcttcct cactataacc atttttcttc atatagtagt
    21481 atctcgagcg aagtgtgttt tttagtatgc catgcttatt gcttaggcat tctaaccatt
    21541 catcaaagta catgccatct ttcattatag tatttctctt attacataaa ttttgcttaa
    21601 cggttacaaa tctgcaattg caaggctcgt aatctccatc attgtctatt ctatcaagtt
    21661 gtaagccttt tttatatcca ttttctttgc accacgaaac gaaatttttg atatcgtgcc
    21721 attctccaca cacttttatt cctctaccac cataattttt atagctttta tgattaggat
    21781 tgtaacatct tctgtgcata gaataaaata ttgccctgca ttcttctttc cctaatccat
    21841 gagtagtatt tctttccaaa agaatatctc ttactaagca accacaactt ttcgtgtgtc
    21901 cactcaatat ttgactttta ggtcttttaa ttatattccc acactcgcat tgaacttcca
    21961 acattctagt cctttttttg gaattttcag acggataatc cgagtattct tttagtaatt
    22021 taaatctagt tccctctatt ttcaatttca acacctcttt aatgaataat atatttatat
    22081 tatagaacta aaactggtgt ttttcaataa attctattaa cgcaaaatta agatttccaa
    22141 ttttaacccg attttcacta tgctatttct aacatagggg gcatggcatt tttacccgag
    22201 gcattcccaa aattttcagt atcttgttgc aacccttgac cactttcata tatttgtttt
    22261 ttcaagattt caagtattat ttttcgagct tctgtaggta tctctatttg catagtttta
    22321 agaccaccac tatctccttc actatctgtt tctgttttta ttgccttgta tctctttaat
    22381 tcatttaaaa attgtgctgt atcctctcca ccataatttt ctaagatgta tattatttgc
    22441 tgtatatcct ctaaatcatt tgcaaaccca ctcattattt tatcgtataa atccaacact
    22501 gatttatatt ttgataagtc actttgtttt tttatgttgt tagcaaattc aataaaagga
    22561 actgaattaa acctgtgtag tattgttata tgctcgattt gagaaccata gcatgatact
    22621 ccaaagaatt tatatttatc taatattttg tctgtccaaa attcaacata agtatatgcc
    22681 tgcttttgta tttgaccttt tacatcctct agctgaatat aatatcttat tacagcttct
    22741 agctctcttt caataccatt tcgatatata ggaataattt cttctgtatt gactacacca
    22801 tatttaaatg tttgattagt tacctgttcc ccactatatt cttcatctat ccagtagtga
    22861 agccatgcag taccacaatt cgacgcttct attgctaaat tcttagcttt tctagtgaac
    22921 tcattcccta gaacatctgt taccttctca ttcaattcct tgttattgtc aatgtcgaat
    22981 aaaactggat aagtaaacat ataagaagct ttttcatcta ctaatatctc atgaaagtta
    23041 tgactaattc tattgtcagc atttcttaat ggattttcat ctctattttg gacaactact
    23101 ccttttttta atatatcatt tttgttataa taatatgatt tagcttgtaa tatctcttgt
    23161 cttctagctg catcagcact tattattgct cttatttttt ctaactccac atcaccacca
    23221 cctattattt aaatactgat aatccttgac cttttaatgc cttttctgca actcctgtta
    23281 tagcatcaca attagacacc aaaaaaccat gagcatagta cacatggttt ccttcaacag
    23341 ttaaattata aacttttact tttttgcttt ctattaatgt ggttgagtct gtttgaacat
    23401 gactttgaac atgtttgtct ttctttggaa gatgccttgt attttgttcc aacaaattct
    23461 gttccacata taatacattt tgatgtatat ttaattcgtc tgtttttacc tctccatctt
    23521 tctccgcatt tatctgaaca gaattgagat gtttgtactt tgctttcaaa atagctccca
    23581 cattctttac acttacattt atatatttta agattttttg aaatctgttt tgcatgctct
    23641 gagtgccatt ttctaccttc ttcactttta tgccattcgg tagctttttc tcttgcctta
    23701 tcaaggttgt taatagttgc tttgtaaaat tcttcatctt caagattttt ctttgcatgt
    23761 agcgataagt gttgtttata cggaatacat tccaagttgt caatgttgtt attaagagga
    23821 ttaaaatctt tatgatgaat atgatagcct tctggaactt cccttttatt attaaactcc
    23881 caaattgcca catgaagtcc tttagcatgt tttctttttt catttttact tgactgacta
    23941 agataatact tcttttgtcc cataagtcta tactcttctc cattgaatat aacagtttct
    24001 tgtacttcca taacatcaac tcctttagtg atattatact agtttctgat atacctgtca
    24061 atttatcaag acttataaaa ccattgatat aactaaatac cttatgattt ctagtagctt
    24121 ttagccctaa tttatttata gtttcttttt ctcctgtaca tcctgaccat aatactcttc
    24181 taaggccaaa tggagtaaat acatattccc cttcttttat tttctctata gatttattac
    24241 caaacaatgt tgatatttgt gttccttctt caaaacacgc atcatcatgt ttatttttcc
    24301 cttctctttg ataactcacc attgccttat aataatcttg ccatctatct ctccaattaa
    24361 ctggaaaata tatgtgttcc attacccacg agctattaga taatattcta gcatttttat
    24421 ttttcgactg atgaaaccat ttaataatag ttttattact tttaaacttt tctttcaata
    24481 ttctttgtac attccttgca aaagctctac caccactatt actttctata tcagctttgt
    24541 taacttcatt ttcatagaac atcttagctg ttttatattc tgttgtttcc atactctctt
    24601 ttgtatataa aacgtctaat acatatgctt ctttgttata cactccataa actatagaac
    24661 ataagtaatc tgctccctca tctgctgtat ctacataagc tttaatagat gtaaatagta
    24721 tattcccttt ttcatcaaca ggaagattgt catatgtctt aaatttagta tatagacatc
    24781 ctttaaggtc gataggttct tgctgatagt tggcacttgc aatgtcttcg cccattgctc
    24841 ttacttttga tttataacta tttagagata agacttcttc acaaagcatg ttgccatctt
    24901 cctgtaatgc tttcatatta atatgtctta ctttcttgcc ttcttctttg taatgttcta
    24961 gtgccctacc tgctaaatct ttgctagacc atctagtcat tataattatt attttcccgc
    25021 cttcttcaag tcttgataac atagtattag taaaccaatc ccaatgcttt tcaagaacat
    25081 tttcgttata agcttcctct gcatttttaa ttaagtcatc tacaatcata agagaacacc
    25141 caaaacctgt tgctgttcca cttggagaag ttgccaaata attattataa ccaccttcta
    25201 atgaccataa attcatagct ccatcaccat gtttgatttt tgtgttagga aatatgtcac
    25261 tatagataat agtgtctata tcagcttttt cttcttgaat agcatttcta acattctttg
    25321 aaaacatagt tgaaagagtt tcattataac taccagtcat tattttttca tttttatttt
    25381 tacctaaaat ccattctacg aataaacctg cacttctact cttcccatgc ctaggtggca
    25441 tatttataat taaaacttca tcatctgaat aataaaaatc ttgaagctta ttacaggttt
    25501 caactaaata cgctctatct tctttataaa agtttggggc taataaattg caaaaataaa
    25561 agaactcacg tcttgcaagt tccttcttag cttctaactg tattaatttt ttatccatca
    25621 tcatctaatt ttgccaacct ttttaattct tctgtagata gttctttaaa tggattattt
    25681 attgtaattt cattagttga ttctactatt tgtttatccc tccattcagc tggttttcta
    25741 tttttcaacc agaatatctg tgctgtagta tctggtacta catgtttagt tactctttta
    25801 gtttcttgac cttcttcata tgttatctca tcatattcat aacctaatgc tctttttaat
    25861 aaagcatttt caacctgcct gtcaattact tcttttccct tttttaaggc attacaaata
    25921 ttactatact tctttttcca gtcatatagt gttttgacat ttattccaat attaaatgct
    25981 atctgctcat ctgttaaacc atctcttgcc catccttcaa tcttaattag tccttctttt
    26041 gttatccagt attcatattt tgccacatca ccacctcgtt tttgtcgttt tgggaataaa
    26101 aaaaagaacc tcataattga gattcttctt ttataatttt cctagttcac attgaagtct
    26161 ataaccttct aatttccaaa gctcattttt aatttgctct tcacaaatat ttagtcctat
    26221 ttgttcatca tagtttgcag ggtcaacaca ggcactatgt cctactaaag taaatccatt
    26281 ttgaagttgt attgtcatta ctgtacactt accccaatac acttccacta tcttttcagc
    26341 ttgattaaac aaatcatcta tttgtcgctg agttattgaa tttttattta taatttattc
    26401 tccttcttaa tttattgtat aaaaaaagac catctataaa gatagtcatt ttagatttta
    26461 tgtactttta tttttttaga tattttaaca tttgaactag atagtcccca tccaatgaat
    26521 ctgtttcgtc tttatcaagg cttctttttc caaagtctac aaatccatta tccttataaa
    26581 attcaattaa tttaggttta tcttcacatt caaggtacac tattttccca cccatatcta
    26641 actgtactgc ttttatcttg tcacatgcaa tttttagaag ttcatcaccc ttgattaatt
    26701 tattataatt atttgaataa ttctttccta tttgccctat caaaggtgct ccaataatat
    26761 atcttcttag ttcttcattg tattgtccaa acttcactat ttttttagct aaagagtttg
    26821 ataatgtttt tctttttatc gtaaaatact tattagctaa agaaaaatac ccaattataa
    26881 caggcttatc tttataagaa gttaatacta aatgtgtact agccaaaccc tgtttagaaa
    26941 attcaatagc tttgtttttt aaaaattctt ctacatcttt attaagggga caagaaaaac
    27001 tggagagaat tttttaactt cttcttcctc cagctctcct agcatattac ttaagtttac
    27061 aattaaataa ccactcatta aaatctccca aatatatcct ttattttatc ttttggtact
    27121 tctgaacatt ttttacttaa tacaacttct ttttcttgtt tatttttagc attttctaga
    27181 gctgatacta gattccttcc aaatgccttt tttcttacat ctacattttt taaaatactt
    27241 tttgtagcca taagtaccac cttccacttc actattataa tttaattata ctgacgtcac
    27301 gtcaaatatg caatacattt cgaggaatta ccagttggca ttttgtacat gattttatta
    27361 ttattattca caatatgtac attttatata ataaaattac tgttatttgt attaatttaa
    27421 ataacttaca ctataaaaaa ttatgtcata tattttatgt atattaattc aaatgcttgc
    27481 taagtattta attttaagta tgcacattat aatataccta aacaaatagt attatgacaa
    27541 taaaaatttc ttttttcttt ttaaaaatta aaaggctaag tttaccccaa acttagcctt
    27601 tttagtaggg agatacatat attatgtcgc aagttctaag aatcgaactt agattaaaca
    27661 ccagcacctg catggtgagt gatttaatag catcatccca agcctcttgc tctgttattt
    27721 tcttttctat agcaattctt tttgctatat ctcttatctt tgttgcacct tctaatatcc
    27781 ccatttcata tttcccccta ttctatataa tttaagcgag gtcataagtc ctcgctctat
    27841 ttaaaatata agttaaacat cttttttaag ttgtaaatca ggaaatggta taactttagc
    27901 agaagttaga gtatttttag gtaaatttaa taaatattct acttcttctg gttctagact
    27961 aaaattatat gaaaatgaaa gttcatccat aaactctttt ggagtaaata ccttctcatt
    28021 taataacatc aaaactgctg tttttaacaa tgctggtaga gaagtcaata attcatcatc
    28081 tagtggctct gattttctta gtcctctacg ttgtaaagta cgaatcatag actgatattc
    28141 atccatgctg ataattccta aagaatatga acgacgaatc attgcttgta tagaaacttt
    28201 ccatttacgt ttcaattctg tataatttgg aatacgtaaa ggagttctct tagcatcaat
    28261 tttaaaagtc tcttctggta ataagaatgt agatgcaaat ctattagctt ctgattctct
    28321 gtctttaaat tcttgttttt ctaaagcttc tacatcctcg ctccactcat gtaagcatat
    28381 atgccctaat tcatgagcta tatcaaaatg aattctagaa gcagaagtct tattactaga
    28441 atatccaatc agataaacag tctctcccga aatatctacc atctgactga atgcatctat
    28501 atcatctgta gaagtcgaga aacttgttac aagtattccg tgttgttcta cttcatatat
    28561 gatattatca ataggtttta accctaatcc ccaagcttct cttaaaagta aagcagcttc
    28621 ttctggtgtt ttcccagaac aatctggtaa atttaatgtt gggaattcaa tataatcttg
    28681 taagaagaaa tatatttctg ctagaaattc cattttttga atttgctctg ctctatattt
    28741 tttatttgta gtcaataatg ctctgaaata tgttgaacct attgcaatat tatttcctgt
    28801 ttctaaaaag aatttaactg ggaaacctaa ctcttttgct attcgtttca ctacattatt
    28861 atcatttggt ttcgatttat tattctcata catcgaaaca gtctgtcttt gacagtcaat
    28921 tctctcagct aattcagcta ctgtcattcc tctatatata cgagctttct ttaaacgctc
    28981 tccattaaaa ttaatttcca taattatttc ctccatctat tcactaaaaa gtgactactt
    29041 ggcatcttcc tcctttttcg ccttttttaa aggatgtgtt ttctttcttg catctgcttt
    29101 tgcagttaac ttcaatccaa gagtaggatt atttgcaaca ggattagtct tatcaacttt
    29161 ttctacaact gtactttctt ctatagatat ataagaactc aaattttgtt ctgatacaat
    29221 atttaaattt gtgtcaacca tcacagcacg aatagatgtc aattcatatt cagctgtttg
    29281 attaaataac aataatacat ggtgttcaat gatagcacca tctttattta tactatcaat
    29341 taatttctgt aaatatattt caattttatc ttcattatca aattctaatg aaaaaaatga
    29401 tagttgttca ataggagctt ttaaatctcc attaaatact ttagttaaaa tatctacata
    29461 atgcattttt tctcttttat tgacattatt atgaagttct ttatatcttt gctctctcat
    29521 taatacataa agatacttgc tatttttatc atatataaaa atcattctcc aacgtccacg
    29581 tttagattta tacgctttaa aattagaact attaaatgca gtatcaattg catcatttaa
    29641 caaatcccaa aactgaaaag gtactccatt tttagttata aggttttgtt ttcctatata
    29701 ttctctaatt tcttcttttg ccttattaaa gcattgaaca atgatataac ctatttcatt
    29761 tggaacttct aattttattt tatttgacac tcaggcatac acctcctaca tatttttata
    29821 tatttattgt aggcgtttta tgcaaaagtg tcaatattaa aagacaaata tatttatttt
    29881 ttatatttaa agcgtcattt gaaaattttt caacttttta tatgatgtcc ttaattataa
    29941 aaattattca ctttggctag aacaaagaca atcccttaac cctagcctaa tatatattta
    30001 gttttgagag ggaaatcttt atttccacaa tattattatc tcacataatt ggcaataaaa
    30061 agtctcacga tagtctccaa aaagtctcaa aatagtctca tttttaaact ttccatgaaa
    30121 aaatgggtaa ttcaaactct tttattttgg gatataacat atccataatt ttacaaacta
    30181 tcctttttct aattctaaag caatgacttc tatctatgtg catagcatta gacatataat
    30241 ccatgttgat tttctcattg ttcatataca tttcattgaa gaactctgtt tcaaagctgt
    30301 tcaaacttgt taatgcacat tctatagttt ccttttcaat ctctaatgtt ttcttatcct
    30361 ctttcaatct atttaaatct tcttctcttt taataacttc attttcaaca cttgaactta
    30421 tattataagt aggtcctgtt ttttcttcat aactttgagc cttacatcca caaaactcat
    30481 tttctaattt tttaatatat atatctttta ttcttatttg actttctagt tttttatagt
    30541 tatacaatct accttcaacc tcttgaaata gtgtcttttt attcatactt ccacactcct
    30601 atcaattatt tatgttttta tgttataata atatttgtat ataaaagttt tatatttttg
    30661 acaagtggag tgtgaaagca ctcctttttt cttttaatta actattgcag gttttcccct
    30721 ttaaagggga aaagtctatt cctgctttaa ctcactattg ataattgact attcaaaagt
    30781 cttatttctt cttcaaacac tataggtaac ttataattgt ttacaatctc taatacttta
    30841 tctaactgac aacgctttat ggccttataa ctatctactc caaactctcg tttaatctga
    30901 tggtatatat cactgtatac tttacctctt aaagatttat ttttataagc cttacttcca
    30961 tgctcaccaa gtgattttgt tgctaccctc ttaacctctt taacaatact ctcacactcg
    31021 atattaaaaa gtggcgcatc atccataaag ttctctaatt tctcattaac attctctatt
    31081 ttagtttcta agacttcttg tttcttatct agcataaata tagcctgcaa ctcctttgat
    31141 gcacttaaaa gaggattatt tagttctttt ctcatagaga aatatccatc aactattttc
    31201 tcgtattgtt cccaagctat atcatcctcc aatattttaa gtagttttga ataacctctt
    31261 tcagataaca agtaaatatt tcttgactga ttaaaagatt gtttactata cccaacatcc
    31321 tcgcaaccta atccttttga aggattagcc tttaaatcac ctagtcgctc agaacgactc
    31381 acttttaaat ctataatatc tatatttgtt ttaaatctct ttatattatt attaattagc
    31441 tcatttatat gtttaagttc tctattatgt atctcagcta tatcttttac taacattgct
    31501 ttcttatgtt ctccaaatcc accctcaatg ttatgaaatt tcattccctc gatttctaaa
    31561 gttccaagta ctgttatttc tttatttata ttttcattca taacctatct ctcctttatc
    31621 atttgatata ttctttattt aactttttca catttttatg aaaaactaag tctatactct
    31681 aacgaacgga tttttttgtt agttaaataa tatcctccaa ttcaacttca actcttggtc
    31741 tatcactgta atatttctta ctcactactt ctactatttg agaatcatct ttataagcta
    31801 taccatttaa actatcagct acagacttga ttatattatc taaatcaggc tttttacttg
    31861 gtcttattaa cccttctact ttagctatag catccttata cgctttagta tttaccttat
    31921 ttttctgtag tgcttctcta tcttttttag taatgtcaaa gtaacaaata actgtcattt
    31981 tcacattacc ctcaaagaaa tgctttactg tagagttgta catgagtcta atccaatttt
    32041 catagagtac agtttggtca ggtgtataag ccttaccatt tgctgtactc attctaggac
    32101 gtgccttcgc ctttggttct ccatctatta caaaattaat tttcattttt caccttctta
    32161 gcttttctct cacatttctt acaacaataa acatccttag atttttcctt aagataaaat
    32221 aacttgccac accaactgca tcttcttcgt ttcataaaat cacttcctat ttagcgtaaa
    32281 tcttctagct ctaagtgaga gtttattttt attaattctt cttctagaac ttccaaacac
    32341 ttatttttat tttttaaaat actatttgta gaacggcatt ttactgtaat atcagttgga
    32401 atatgagtaa cttcaacaga ataatcttta tttttcacca ttttcaattc tttaggatgt
    32461 atagtatatc cgttttctaa tttatataac tcatttttac cttcaatgta actttcacac
    32521 actttcaagt tattaatttc aagtctttca agcatacaca tatcttcaaa gtagttttta
    32581 caattataat tttcgcaata tatattagcc atttaataca ctccttttta taagtcaaag
    32641 taagcctata gacttctagt ttcattcaca aacttacctt gactatttta ttctatttac
    32701 ttcttaattt cttatagttc tcttcacata ctttatccat attttctttg tatttgcatc
    32761 ctatacacac attgcaagtt ataaggttat tgctaattac aaagtcatag ttgacacatc
    32821 tttcatcaca ctttttatga attaatcctt tttccatttt aattctttta ttcatattta
    32881 accctccctt agcttctcaa ttatctcaat ttcatcatct gaaaatacca tactagcttc
    32941 cccatcacaa ctaccatttt cccagtcttt tttaaatcgt tcatattcat ttatataaca
    33001 tccaaaatat tcatcttgtt ctaaaatata atatgcatct cttatttttt ctacgtcttt
    33061 ttcatcacag ttagctaaac aatatacatc tttaaactta attacagtta cttctaaacc
    33121 atcaattaaa ttattatatt ttaatgctct ttttaatcct tcttctgaac tcccaaagtt
    33181 tatatctggt tgatttctta attgttctaa tgttttttta gtctttaatt ttactttata
    33241 agtactatac atagtaaata tcctccaacc aatttttata tgctgtatca caatctttac
    33301 tttcacaatc tatcttagtg tttttgcaac taccacaaat ctctttacta aatcgcttat
    33361 aactttctgt atctgcaata ttctttgact cgcacatttc tttattagtc atatgagcac
    33421 ctacttttca ttataaaaac ttacattttt aataattata tctacagttc cattcccatt
    33481 atgtctaatg ccatacttca taaaatcctc aaaatcatcc attttgcctt ttatctcaat
    33541 ccctgtatct gtttttatat gtctattttt tagatttttc tcaacccatt ttttatcaat
    33601 attaaaactt ccatcaagac ctttttcttc tgcatggtcc ttaaaactat cttttaaatc
    33661 atcctttatc actttatcag caaactcatt tatatcaagt ttttgctttt ctcttagcat
    33721 gtaaagtaag atacttctta cgtcttctcc ctgcttcata tcactatata aattcgctat
    33781 aaaagcatct accaagcctt taaacacctt agttttatac ttatcatctt tcactttagt
    33841 agcatttaaa aactctgtaa caaacttaga attagtttct tccttttctg catctttatc
    33901 taaaacactt agatgatact cgtcattcat tccactcaaa ccaataattg cccctatttt
    33961 aattgcttta gtctcttgta tattaatttc atttttagac atctgtatgt taaatttatc
    34021 atcttcaaag ccaatagaat gagtatacga attattgtaa tcaagcttta atatagcaac
    34081 tttcttttca tctttttcag tatataaaca tattgctaag tcgcaagatt ctaatgtagc
    34141 attcaatttc ataacatcaa ataaataagc tgcaatctct ttagagttat ttaaaaatga
    34201 actttcatca taaataattt gttcgcaaca cttcttaatt agattgttac tatagtcatt
    34261 aaatactgct gttctgatat cattatctct tgatactttg cttattttct tttgaaaaaa
    34321 taggtccata tcttgactga ctctaccttc aaaatcgttc aatattggtg tatcgctatt
    34381 cttatctaaa acatgtatta taaatttgtg tattatcata attccacccc ttttaattat
    34441 tttctccaat tccaaaccag taccaatcta taagattttc acagatgtat tcttgagcta
    34501 tttttttcat atattctgtc ttttcttcat cactcatacc ttttaaatca tcatcatcta
    34561 ctttaatttc accatgagta tacgaacctt ttttattagt tttaaccatt acacatatca
    34621 gcataattcc accccttatt ttcatttttg agagccacaa aacacttcaa caattaattt
    34681 atattaaaag acattttgca actctctaaa ctgttttaat tagatatttt ccctatataa
    34741 aaatcctttt aatctttcta tttctttgct tatttcatca ttccaaattt ttatagctct
    34801 acctccatgt caagccactc tttgcatcca tcaacacatt tttcatctac atcacaaatt
    34861 tcatatcgat attgacaact gcagtaaata caagtacaat tatcaccaat ttcgtttata
    34921 aattcagcca actctttttt actcatattt tttatcattt caaaattagt cattcttaac
    34981 ctcctctttt tctctcaata atttacgagc tttttcttca tcataactgc aaatatttct
    35041 accatcgcaa taatgacaaa gtaatctagt atttttagta acgttaggaa acattttttt
    35101 gcaaatctcc aatctatcat ccattcttat acacctcttt tgttatcaca attttcacac
    35161 tctttcagat tcaatctata ctcataaacc ctaccagcta taaaactaat tcctatcaaa
    35221 agtaaactag ttaagatatt cattagattt catccttcct atcatcaatt aatatattaa
    35281 acccacattt acattctcta taatatgtgt gttcttcaac tattaatttg ccttcattgt
    35341 ttcctatttt gtcattacca caactaggac aatagcaata cttttctcca agttttataa
    35401 tatcttttaa tttcatttct caatatctcc ttgaactgtt ttactttttc ttttacgagt
    35461 taacatttgt gatacaaatt cataaaacaa tttctctaac tcttcttttt ctgaatttac
    35521 acaaactttg aaactctctt tatctttagt taactgcatt tgtattttca ttttcaagtt
    35581 tctccttaca ttccaagaac tcttttctga cttcctctaa gttctcatat tcattaccaa
    35641 ctactttgta atctctacta tgatatattt ctgttttatt tataatctct atccattctc
    35701 cttcaaactt ctttaaataa tgccactcta aatacaattt aatacttcta aaatctcttt
    35761 ttacaactcc atattggctt gtatcacgag gattataata ttctttcaat atatcccctt
    35821 catagatttc ttgattattt ctatcccatt cctcagaaca tattccaaca tcacaaactt
    35881 ttcgccattt aaaatcttct ttcagcatta ataaacagtc tacaagatta ctccacatta
    35941 tcgttttaga ataaatccac tttttatttt caaaatcata acctctgtat tcaatcaaac
    36001 tcatttaatc atcccctcat attcatatct acttaatatt tttatagcta tatcaatagc
    36061 tttatttaca gaacactttt tcttatttat tatcttttca gctaatttaa ttacttgttc
    36121 cacatttgct agtaccattt gacgcctctt taatatatct agctttccaa ccatttttag
    36181 tagtcttgcc tgttcttgct agatacccaa cataaagttc tgtaaaatat aaacatttac
    36241 ttgcttgaac agcattatca aacaccttac gctctccagt aatgatgtta gtacactcta
    36301 ccttcttacc ttttcttcct tgccctttgg aatttattct accaattaaa tttttttgtt
    36361 tatttttatt tatcatttct cttagattac attcaatatc tcctatatct ttagtttcca
    36421 aaaatgttcc tgcatccatc caaactttag ccatttcttc gtccccccct atttaactgg
    36481 catttgaaat attctatttc tataacttct aaccttataa ctgtctatag aatccgttct
    36541 agtcccacct tcaataaatc tttgtatatt atctaatacc tgcatagctc ttttttcatc
    36601 ctcatatttt cctatttcct taaagttgtt tatttctcca aacatggcat atacatattc
    36661 tttatcaaca tttatccaat cagctttcac taaatctgtt ttatcttgac ttctaattat
    36721 tatcatctct aataccccct accttattta acttcaacac accattccga aattacacca
    36781 aaattaaacc gaaattcatt tggatagcaa acatagccat catcaaaagt tttttcaaca
    36841 gtaaattcaa caccttcaat atcatcaatc caaagttctt tatattcttt atattgttct
    36901 acaccattaa tttccataaa tttctcttta tcaaacttat aagttttacc tatctcaaat
    36961 ctcatattgt tgttcctcac attttcttaa tagttcttct aagcaaccct tgcatataac
    37021 aattacaaat tctctgccat acaaatctac aactttagca tttgtaaatt catcaccata
    37081 actgtcaact aaaaactccc cacaaacact acatatagct gttctactca tttttatccc
    37141 tccattttta acttaattaa aaaggtatat cgtcatcatc tattgcttga aaaccttgtg
    37201 ggtctaatcc tggtggtata tattcctgtt gtttttcatg attattacta tcctctttac
    37261 ttgaaagcaa ttctaaagca tttacattaa ccttagtaat agatttccaa caaccatttt
    37321 catctttgta attatctata tttaattctc caaaagcata tatctgttta ccttttgtaa
    37381 gatactgtac aagattttct acatgttttc ctaattgctc acattgaata aaatcagtta
    37441 ttttatttcc attcttatct ttaaaccttc tatctaccgc tattgaaaat gttatttttg
    37501 gagtacctga attaggaagg tacttcaatt ctgcatctgc aactaatctc ccaactaaag
    37561 ttattgtatt catttaaata gccccctttt tatttttctt catgttcttc tgtatactca
    37621 acaaagtaag tgtaagttgt cttgctattt tgcttctctc tagcaatctt tactgtatat
    37681 ccagctttcc caagtaatct taataattcc aatctatctt gttcatttaa agaaccactt
    37741 ctttgtgcat atattctcgc cattttatac ctcccctttc taggaagcaa tatattgata
    37801 tttacttcct agaagtttaa ttttatttaa atttttcttt ctgactcttt ttaataatct
    37861 cgtctagctc tttttcatca tattgagtga aagtctgatt gaagttagca aacttatttt
    37921 tacttagttg gttattaact actttttttg ttttcttatt ttcttgttgt aatctatatg
    37981 actccagttg ttcatatgta gttatatttg catccttcca cttttttagg atacctttta
    38041 aatatgctag attcatattc atcttttcag cacatatctc tatagctctt ttaaatactc
    38101 ttatatctac ctcattagaa atctcaacta accattcagc tgtaacagga tatattactc
    38161 ctatattttc ttcatatagt ttcttaaatt cttttaaaga gttatccaca ggttgctttc
    38221 ctatatacat attattaata ctgttactat taatactgtt actattagtg tccatatttt
    38281 ccgtgtccgg ctgagtcgtg tccggaaaag taggacatgg ttccatgtct acatttttag
    38341 gatacggttc cgtgtcttgt tttttaggac atggcttttt cttctttttc tcttctttta
    38401 aacctcttct agatatacat tcatctatat aagttctatc aaatactatt tcatatatat
    38461 tattttgcat tttaccttgt ttggatttat tcttatgaat ccttatataa ccactcatct
    38521 ctagttcttt tttgtacttt gtaaatgtgt cttttgatat atctagttca taacaaatta
    38581 aatccctaga tggaaaacat gttccatcat tccctgcaaa actagttaga tatgaataca
    38641 gcattctagc accaactgtc aaccacctat ctcttgctat tattcttggc ataagaccat
    38701 atccaccact taatatgttt aatttctcta aaactgactt atcttctttc aaggtgtctc
    38761 acctacttaa tcaacattta tagcttcttc catacttatt tggcaatcta tattttcagc
    38821 ttcttctttt acttcaactt catcttcttt cagctcttca aactcagcat ctataaaatc
    38881 atcttttggt tcatactcgc ttagtaattc aattaattca tctacttcct caaattttag
    38941 ttcttttaaa tcgaatccat tactttcaca aaattcctct agctttgatg tatctttttt
    39001 attctcataa tcatacaaac ctttcattga tgctaacttg agtattcctt gtttttgtga
    39061 tggacttgcc ttacctattt caataggttt ctgaggtaat ttatcgggta cagtctttat
    39121 ttctgcacta tcatataatc cttgtaaatc ttctggaaat gcttctctta aagctgtaac
    39181 aatagcacat tttcttatca tcacacaagg catttgtttc caagtggctt ggctctttga
    39241 atactcttct aaagacacta cagactttat aggaaacttc attcctctaa ctgatacttc
    39301 acaccatcca ccgattagtt cttcttgtgg cagctttaaa ctgccctctc tttcaaatac
    39361 ttctccattt ttatttacag ttactattcc tgctttcata ccttcaaaat taggattttt
    39421 atttgctctt tttacaaata catctttacc tactactata tttgctggcg aattaccaaa
    39481 ctttattaaa tatgcttctt ttatgaatgg atttaatttt tgagccttac ataattctat
    39541 aaacattagc acttcttggt ctgttacatt tccatttcca cttactaagt aattctttac
    39601 tgtcatgtaa tcaagtattt gacccgattc taaggtgcat gttgctaatt ctaaagcctt
    39661 gttattcata ttaattcacc tcttttttaa cttttggagt tgttaatgta gttgaatatt
    39721 caatcctaca accttcaacc tcatgacctt ttttgataaa atctttaatg gtatttttat
    39781 ctactttcac aatttgctct actgttttat atatactagg tatcttttct tcatcttcta
    39841 taactaagct acctgctgac tttcttatac ttatatttcc taaaactgtt tctacttttt
    39901 tagtaccaag tagttccata cagtctttta tattgctttt taatctatca agagtattct
    39961 ttttgaccct ctttaactct tgtaatcttt taatctctga atctatagag tttatatcgc
    40021 tatcgatatt tattatt
//
LOCUS       DAEMHW010000012        39783 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEMHW010000012 DAEMHW010000000
VERSION     DAEMHW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39783
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.012"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            24..2672
                     /gene="polA"
                     /locus_tag="KPK67_000536"
     CDS             24..2672
                     /gene="polA"
                     /locus_tag="KPK67_000536"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBG2457543.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKDYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEVLKLKDTEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            2681..3283
                     /locus_tag="KPK67_000537"
     CDS             2681..3283
                     /locus_tag="KPK67_000537"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBG2457544.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNRNISIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            3276..3830
                     /locus_tag="KPK67_000538"
     CDS             3276..3830
                     /locus_tag="KPK67_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG2457545.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            3830..5545
                     /locus_tag="KPK67_000539"
     CDS             3830..5545
                     /locus_tag="KPK67_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2457546.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWQDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            5618..5830
                     /locus_tag="KPK67_000540"
     CDS             5618..5830
                     /locus_tag="KPK67_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBG2457547.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            6031..6630
                     /locus_tag="KPK67_000541"
     CDS             6031..6630
                     /locus_tag="KPK67_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457548.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            6858..7229
                     /locus_tag="KPK67_000542"
     CDS             6858..7229
                     /locus_tag="KPK67_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2457549.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            7361..8635
                     /locus_tag="KPK67_000543"
     CDS             7361..8635
                     /locus_tag="KPK67_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG2457550.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            8746..9690
                     /locus_tag="KPK67_000544"
     CDS             8746..9690
                     /locus_tag="KPK67_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457551.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSTKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            9906..11243
                     /gene="rsxC"
                     /locus_tag="KPK67_000545"
     CDS             9906..11243
                     /gene="rsxC"
                     /locus_tag="KPK67_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBG2457552.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            11266..12243
                     /locus_tag="KPK67_000546"
     CDS             11266..12243
                     /locus_tag="KPK67_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBG2457553.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            12243..12812
                     /locus_tag="KPK67_000547"
     CDS             12243..12812
                     /locus_tag="KPK67_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBG2457554.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            12825..13418
                     /locus_tag="KPK67_000548"
     CDS             12825..13418
                     /locus_tag="KPK67_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBG2457555.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            13433..14008
                     /gene="rsxA"
                     /locus_tag="KPK67_000549"
     CDS             13433..14008
                     /gene="rsxA"
                     /locus_tag="KPK67_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBG2457556.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            14024..14995
                     /locus_tag="KPK67_000550"
     CDS             14024..14995
                     /locus_tag="KPK67_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBG2457557.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            15215..15799
                     /gene="maf"
                     /locus_tag="KPK67_000551"
     CDS             15215..15799
                     /gene="maf"
                     /locus_tag="KPK67_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBG2457558.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            15844..16503
                     /gene="radC"
                     /locus_tag="KPK67_000552"
     CDS             15844..16503
                     /gene="radC"
                     /locus_tag="KPK67_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBG2457559.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            16624..17634
                     /locus_tag="KPK67_000553"
     CDS             16624..17634
                     /locus_tag="KPK67_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG2457560.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            17644..18537
                     /gene="mreC"
                     /locus_tag="KPK67_000554"
     CDS             17644..18537
                     /gene="mreC"
                     /locus_tag="KPK67_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBG2457561.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            18552..19040
                     /gene="mreD"
                     /locus_tag="KPK67_000555"
     CDS             18552..19040
                     /gene="mreD"
                     /locus_tag="KPK67_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBG2457562.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            19052..22030
                     /locus_tag="KPK67_000556"
     CDS             19052..22030
                     /locus_tag="KPK67_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBG2457563.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            22128..22811
                     /locus_tag="KPK67_000557"
     CDS             22128..22811
                     /locus_tag="KPK67_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBG2457564.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            22835..23632
                     /gene="minD"
                     /locus_tag="KPK67_000558"
     CDS             22835..23632
                     /gene="minD"
                     /locus_tag="KPK67_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBG2457565.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            23650..23934
                     /gene="minE"
                     /locus_tag="KPK67_000559"
     CDS             23650..23934
                     /gene="minE"
                     /locus_tag="KPK67_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBG2457566.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            24019..25149
                     /gene="rodA"
                     /locus_tag="KPK67_000560"
     CDS             24019..25149
                     /gene="rodA"
                     /locus_tag="KPK67_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBG2457567.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            25190..25603
                     /locus_tag="KPK67_000561"
     CDS             25190..25603
                     /locus_tag="KPK67_000561"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBG2457568.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            26088..26417
                     /locus_tag="KPK67_000562"
     CDS             26088..26417
                     /locus_tag="KPK67_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2457569.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            26421..27212
                     /locus_tag="KPK67_000563"
     CDS             26421..27212
                     /locus_tag="KPK67_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457570.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYESEADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFVFGLLCIIAYFILRFKISLIKNNN"
     gene            27776..28858
                     /locus_tag="KPK67_000564"
     CDS             27776..28858
                     /locus_tag="KPK67_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG2457571.1"
                     /translation="MKKIRKLATKLLIAMMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIAEQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVSVIGFMNTPYGMVALAPDGVPVEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETTSNSGIGLVAYARYMN"
     gene            complement(29062..30255)
                     /locus_tag="KPK67_000565"
     CDS             complement(29062..30255)
                     /locus_tag="KPK67_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG2457572.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDKFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            30574..32418
                     /locus_tag="KPK67_000566"
     CDS             30574..32418
                     /locus_tag="KPK67_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBG2457573.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLVDFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNRDGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            32420..33121
                     /locus_tag="KPK67_000567"
     CDS             32420..33121
                     /locus_tag="KPK67_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBG2457574.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            33139..34560
                     /locus_tag="KPK67_000568"
     CDS             33139..34560
                     /locus_tag="KPK67_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBG2457575.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            34661..34972
                     /gene="rplU"
                     /locus_tag="KPK67_000569"
     CDS             34661..34972
                     /gene="rplU"
                     /locus_tag="KPK67_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBG2457576.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            34987..35304
                     /locus_tag="KPK67_000570"
     CDS             34987..35304
                     /locus_tag="KPK67_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBG2457577.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            35318..35608
                     /gene="rpmA"
                     /locus_tag="KPK67_000571"
     CDS             35318..35608
                     /gene="rpmA"
                     /locus_tag="KPK67_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBG2457578.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            35719..36996
                     /gene="obgE"
                     /locus_tag="KPK67_000572"
     CDS             35719..36996
                     /gene="obgE"
                     /locus_tag="KPK67_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBG2457579.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            37048..37416
                     /locus_tag="KPK67_000573"
     CDS             37048..37416
                     /locus_tag="KPK67_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBG2457580.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     regulatory      37508..37614
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            37717..38922
                     /locus_tag="KPK67_000574"
     CDS             37717..38922
                     /locus_tag="KPK67_000574"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG2457581.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNEDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     gene            complement(39123..39689)
                     /locus_tag="KPK67_000575"
     CDS             complement(39123..39689)
                     /locus_tag="KPK67_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2457582.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
BASE COUNT        15623 a         4340 c         7579 g        12241 t
ORIGIN      
        1 ataattttta ggagtgatat aatttggaaa aaaaattaat tataatagat ggaaattcaa
       61 taataaatag agctttttat gctcttccag aaatgaataa taaagaaggg ttaaagacca
      121 atgctatata tgggtttact acaatgctat ttaaaatgat agatatatat aaaccaacac
      181 atataagtgt agcttttgac agaaaagcac ctacatttag gcatttagaa tataaagatt
      241 ataaggctgg aagaaagggt atgccagacg aattggcaga acaattacag ccattaaaag
      301 accttttaga taaatttaga ataaatagac tagaaataga tggttatgag gcagatgata
      361 ttataggtac tgtttctaaa aaagcagaag aaaatggata taaggtatac atagtaactg
      421 gagataaaga tgctattcaa cttgcttcag ataatacaac tacccttata acgaaaaaag
      481 gggttggaga agtagaagaa tacaacttta attctgtaat tgaaaaatat gagatgactc
      541 caacacaatt tatcgattta aaaggtctta tgggagacaa gtctgataat atacctggag
      601 tacctggaat aggtgaaaaa acaggtataa agcttataaa agagttttct agtatagaga
      661 atttaataga gaatacagat aagcttaaag gtagtgtaaa aaagaaaata gaggaaaaca
      721 aagaaatagc tattcaaagc aaaagacttg caacaattat aagagatgtg ccaatagaag
      781 tggatttaga tagtatgatt tttggcgatt atgataaaga tgaactttta gataaattta
      841 gatactttgg atttacaagt ctcttatcaa gattagttga tttagtggat tcagatgaca
      901 cagaacacgt agaagaaaaa atagaagtat taaaattaaa agatacagaa aaatttatag
      961 atgaagtaaa caaaaaagga caagttattt taaaaactgt tagaggacaa ggtaatatac
     1021 ttgaaaaaaa tataatatat atttttataa gtgtagatgg agaaaaaata tattatgtag
     1081 attcaaatga agtgtcaaag ttagatagta tattttcaaa taatgaaatt aaaaagtatg
     1141 gatataattt aaaagatgat tatatatctt taaaaccata taatataaac ttagaaaata
     1201 tgtattttga tataacaata gctgagtact taatagattc aacttcatct acctcatatg
     1261 attgtagtgc aatagctatg aagtatttat caaaaaaggt taaatcaaaa gaagaattat
     1321 taggaaaggg tgttaaatct aaaaactatg aggatttaga atttgaggat ttagcagagt
     1381 atatgggtca aataatatgc acagttaaag aaacagttcc tatgatggaa agaagtctaa
     1441 tggatatgag tatggatggt cttttttatc atgtagagat gcctttagta gaagtcttag
     1501 gtcatatgga gtatgaaggt ataaaagtag acaaaagtat attggatgaa ttgagtgttg
     1561 aatttaaaga aataatagct acattagaaa aggaaattta tgaattatca ggagagccat
     1621 tcaatataaa ttcaccaaaa caattgggtg ttatattatt tgaaaaatta gagcttccaa
     1681 taattaaaaa aactaaaact ggatattcaa ctaatgcaga tgttttagaa aaattaagag
     1741 ataagcatcc aataatagat aagataactg agtatagaca aatagtaaag ttaaattcta
     1801 cttacgttga aggactttta aatataataa atccagaaag taatagaata cattcatcat
     1861 ttaatcaaac tataacgact acaggaagaa tttcatctac agaaccaaat cttcaaaata
     1921 tacctataaa aatggaaatg ggtagaaaaa taagaaaagt gtttattgca gatgataact
     1981 gtaagttggt tgatgccgat tactcacaag tagagctaag agttcttgca catatgagtc
     2041 aggatgaaaa tatgatagaa gcatttaaac atcatgaaga tattcatacc aaaacagctt
     2101 cacaagtttt taatgttcct atggaagatg ttactagtga attgagaggt gcagctaaag
     2161 ctgtaaactt tggtattata tatggtaaaa gtgactttgg tctagctgat gatttaaata
     2221 tacctgttcc aaaggcgaaa gaatatatcg aaagttattt tgctaaatac cataaaataa
     2281 aagagtttat ggacacaaca gtagaaaagg ctagtgagga tggatatgct gtaacaattt
     2341 taaacagaag aagatatata ccagaaataa agtcaagcaa ttttatggaa agaaatagag
     2401 gaaagagatt tgcaatgaat gcaccaatac aaggaagtgc agcagatata ataaaaattg
     2461 ctatgataaa tgttcataaa aaattggaag agaacaattt gaaatcaaaa cttatactac
     2521 aagttcacga cgaattgata gttgaagcaa ttgatgatga aatagatata gttaagaaaa
     2581 tagtaaaaga agaaatggaa aatgctgtaa atatggatgt tcacttagat gtagatttaa
     2641 atgtaggtag ttcttggtat gagacaaagt aggtgatttt atgttgatat taggacttac
     2701 aggaggaata ggctgtggaa aaagcagcct atcaaatata tttagaaatc gtaacatttc
     2761 tatagtggat gctgatatta tatccaggaa gatatttgag gataagttgt tattggaaaa
     2821 agtatttgtc cattttgggc aaagtataaa aaatgatgat ggaactctaa atagaaaagc
     2881 tcttgggaaa atagttttta gtgatgaaga aaaacttaag gaattaaata acttgactca
     2941 tccacgaata agagaaaaaa taataagtga aatagaaaaa ttgagaaaaa aaggcgaaaa
     3001 tatagtagtg ttagatgcgg caatattggt agaaagtggt tttttagata tggttgacaa
     3061 gctattagta gttacttgta aacaagaagt acaaattagt agaatacaaa agagagataa
     3121 ttgcagtgag caggaagcac ttagtaggat aaattcacag atgagtcagg aagaaaaatc
     3181 caaatatggt gattatataa tagataattc gggaacaata actgaattag aaagtaaagc
     3241 acataaattt attgagtaca tgaaggagaa ttggcgtgaa tagtaagaaa gtattgattc
     3301 tatctatttt tataatctta tttggggcac tattaatgga aagcaaagta atacataaat
     3361 ttttatatcc taaaaaatat tcagagtatg tagaaaagta ttcgaaagaa tttaatttag
     3421 atgaaaatat agtttacagt gttattaaag ccgaaagtaa gtttaatagt tctgctgttt
     3481 caaaaaagga agcaaaagga ttaatgcaaa tattagacat aactagagat tggggagcag
     3541 aggaactaaa tttaaaaaat gtggatattt tcgacccaga gactaatata agacttggct
     3601 gttggtattt aagtaagtta tacaaagaat ttggtaaatt agatttagtg atagctgcat
     3661 ataatggtgg ttcaggtaat gtgaaaaaat ggttagaaaa taatgaatat agtaaagatg
     3721 gcgaaaatct acatgatata ccttttaagc aaacttcaaa atatgtagaa aaagtaaaaa
     3781 ataattacga acattataat aagatatatg gcaagaaagg aaaaaactaa tgaagagaat
     3841 aaaagtctta acagtcgttt tagctataac tcttatggta gctggttgta gtaatactaa
     3901 aacaaaacaa agtagtagtg attcaagttt acaaagtcaa agttctatag atgctaagta
     3961 tataaactta acaatggtaa ctccaaaaac tataaatcct ataactaata ctgataagtc
     4021 tgttggttat ataatgaatt tagtttatga tagtttattt actatagatg agaactacaa
     4081 tgtaattcct caattagtta aggagtataa tatagctcag gatggaatga gtatagatat
     4141 aaagctaaaa gatgcaaagt ggcaagatgg aaaaaatgtt acttcaaatg atgtaaacta
     4201 tactattggt ttaattcaaa aaagtgcaga tagtccatat aatgaattta ctaaaaatat
     4261 agcttctgtg aatattaaat cagataaaga ttttacaata aaatttaaag ctagatatgc
     4321 tttttcaatt gatagtttaa tttttccaat tgtatctcaa aatcatttag attcaaaaga
     4381 tgtaaatgac aagaataata atatgatagg aaatgggaaa tataaaatag aatcatatac
     4441 agaaagagaa ggaatggttt tatctgtaaa caaagattac tatgaagaag ttccaaagac
     4501 catgaagaac ataaaagtag gtatggtgcc taatgaagat gcaaggacat ctatggttat
     4561 ggctcttgat agtgatataa ccaatgttac attaaatgat ttaagtaagt ttcaagaaaa
     4621 agagtttaat ataacaaaat atcaaggaag agattatgag tgtgtattat ttaattataa
     4681 taatcctttt ttcaaagatg ttaattttag aaaagcaatt gcacactcta tagataaaga
     4741 tagaattatt agtgaaggtt atatggatga tgctactcca gttaattttc cacttaattc
     4801 aaaatcaaaa tactataata gtgaaatgaa ggatttagag tttaataaag ataaggcgat
     4861 agagtgtctt gctaaggtag aatatgcaaa tgtaaactca gtaaatcaaa atgacaataa
     4921 agtaaaaaat cagaaaaaat cagctaaaaa gaactcaaag aagcttacgc cagaagaaga
     4981 agcaaaagct aaaaaagcag aagaagatag aatcaaaaaa gaagctgaag agaaaaaaaa
     5041 tagagaaaaa gaggaagtaa agaaaagtct ttctgaaatg aacttaaaga taatagtaaa
     5101 cagagataat agtgagagaa taaaaactgc aaacataata aatgaaaact taaaagctat
     5161 aggaataaat agtacagtaa atcagctgtc agataaggat atggaaaatg ctcttaattc
     5221 taaaaattat gatttagcat tagttggatg gaaactatca attataccag atgcaagtag
     5281 tattatagcg agtagtggat atactgatga taagttaaat ggatatatgt ctgcacttac
     5341 aagttctacg tctgaaattg aaacaaaaaa agcatacaaa gatgtacaga catatataaa
     5401 agataatgca acatttataa gtttagctgt gagaaataac tatattgtaa gtaatagtag
     5461 attaaaaggt aaaattactc caaatgattt tgatgtatat gagggaatat ctaatttaga
     5521 tataaaatcg aatgaatcaa attaaatgat taatttctat ttaaaaagta taaactatat
     5581 gatagaatac ccttataggg tataaggagg taattaaatg aagaagaaac tattagtaga
     5641 aggtatgagt tgtggtcatt gtgtaaacca tcttaaaact gcattaacag aagatataga
     5701 tggtattgaa gttttagatg tagatttaga aaataagtgt gcatctgtag atatgaaaga
     5761 tgatgtatct attgaaaaat taaaagaagt gatagcagaa ttaggatttg aactaaaggg
     5821 aattgaatag aaattgataa actaaaaaaa gacctctatt gggggagtaa tagaggtcta
     5881 aaaatggggg agattgagta tgtttgattt tacattacta ttatctcccc ttttaaatta
     5941 atatatacat aaaaagtaaa aaaattttgt aatgactcac atttcttgag ctttttgaat
     6001 atactataaa tagctaaggg agggagtatc atggaatcta ggggatataa agaggattta
     6061 aaaaaaatga tacttgatat ggcccacaaa gaacttgaga gttatgtatc acaaaaagga
     6121 gcaaaaaaat actttgaagg taacttggat aagattatta ataataatat aaaaaaattc
     6181 aatcaaagta ttggtcttaa tagagtatat ttaaaaatgt taaaaaagtg tgcatatcaa
     6241 gaaagtgtaa cttcaatatc aaggactata aagaaagaag cattatttaa atcaaaaaaa
     6301 gagctatttg ctttttcaga gtacttaaaa cttgatatta acaagcgact ttcatataat
     6361 caaattttaa gaaaaatatc aagatatata tactacaata gaagttcata tgctcaaaaa
     6421 tacgttgttt tcaaaagagg agatgaagaa tatcttttag aaccagagaa aataaaagat
     6481 gaattaatat caagctatag gtcaaagact agagaagata tgaggtcaat agcaaaactt
     6541 ttggatatag aagtagaaga tgattataat gctgaagata ttagaaaaaa agtaataaat
     6601 tatattatca aagaaaaact taaaagttag agttttaaag aataagttct gccaatttgc
     6661 atgattaagt ataacttggg tataaatttt ttatgcattt gaaagagctt attctttttt
     6721 tgacatattc ttttaaataa tttaccatga tttggtaatt ggtcatcatc attcaaattt
     6781 ttaacttaat atgatttaaa taaatttata ataatgatat aatataaaaa aaataaaaat
     6841 tggagagtga tttcaaaatg aagtttaatt tttgtcataa caatttcaat gttactaatt
     6901 tagaaaaaag tttagatttt tataaaaaag ctttagggct taaagaggta aagcgcaagg
     6961 aagcagaaga tggaagtttt atattagttt atttaggaga tggcattact agtcatacat
     7021 tagaattaac ttggcttaga gattgggata gaccatataa tttaggagac aatgaatttc
     7081 atctggcttt agaagttgag gagtttgatg aggcaaaaaa acttcataaa gatttagatt
     7141 gcatatgttt tgaaaatgaa agtatgggaa tttattttat agcggaccca gataattatt
     7201 ggattgaaat acttcctaaa aatcactaat tttaaggata tgtttataat ttctttacaa
     7261 ttaataagaa aataagttat cattaaattg tagggactaa aaatggataa taatgttatg
     7321 aatttatgac tataaaaatt tgttgggggg agtgatagta gtgaaaaaag ctatagcagc
     7381 tttaggaatt ggggcagtag cagtatctgt tagctccata aatgcaagtg ctcttgaaaa
     7441 gggaacagta acagcaagtg ccttgaatat aagaagtgga ccaagttctg attgcgataa
     7501 ggtagcaaaa ttatacaagg gaaaaactgt agagatttta gaaaagtcta atggatggta
     7561 taaagtaagg gtatcgagtt ctgttgttgg atggggaagt gcaaaataca tatcaacaag
     7621 tggttcgtct gaaggtacat caagtcaaaa taatccaact tcaagtggga caactattag
     7681 tggaaatggt aaagtaaatg tgagttctag actgaatgta agaagtggtg caggtactaa
     7741 ctattcatta gttggtaaag caaataatgg agatgtagta aagttactag aacaaagcaa
     7801 tggatggtat aaaataaaac tatcaaatgg agttactgga tgggctagta gccaatatat
     7861 ctctaaaaca tcagaggatg ttggaacgaa taattctagt aattcaaata gtactaataa
     7921 ttctgataaa aaaccatcta gcgaagaatc aattgaaggc aagaatggta aggtaacatc
     7981 tgctgtgagt ttaaatgtaa gaagtggtcc tgggacaagc tattctataa taggaaaatt
     8041 aaatggtgga gatgttgttg aattaaaagc taaaagtaat ggttggtata aggtaaagtt
     8101 atcgagtgga acaattggat gggtgagtgc cagttatatt tctgagacta atgaggatac
     8161 aaaagaaaaa ccaaattcat catctaacca aaattctcaa tctaatagta actcaaagcc
     8221 atcatttact ggaaattcag ataaatctac agcaaaaggt tcaacaatcg tagattttgc
     8281 atatactctt attggaatac catatcaatg gggagcttca gggcctgata aatttgactg
     8341 ttctggattt acacagtatg tttttaaaca ttctgtaggc gtttctattc ctagagtttc
     8401 tagagaacag gctaattttg gaagtgcaat ttctatgggg aattatgcac caggagattt
     8461 agtttatttt gacactgatg gtgatggaac aactaatcat gtaggtatat atgtaggaaa
     8521 tagtaaattt atccattgta gtggtactca gactaatcct aataaggtta aagtggataa
     8581 tttaactagt tcatattggt ctaaagtatt acttggtgca agaagatttg tttagtaagt
     8641 acaatttctt attaaaaaat tagaagaaat aattatttat attcataaat tgtgactctt
     8701 aaaaatatac tttattaata atataatttt gggagggaca aactaatgaa tagaagaaaa
     8761 acgatataca gaggatttaa taataataga cataagattg atttacgtaa atatgttata
     8821 acgattgctt gcttgtgtct aattggctat tattcttata caaaaataaa agatagcaag
     8881 atattggagt atgtatcgac taaaatacca tttctaaata attcatcaga tataacttat
     8941 aaagatatat ctgatgaatt aaattcaata aaaaaaggga aaaaatctaa atcacaaacc
     9001 aattcagatg ataaacaaga aactaatcca gaaaaagcgg taaataacac taaagagcca
     9061 gaagaagtca aattagctac tatagaaggc tgggatatgt atactattca agttgctgca
     9121 atagataata atgatgattt gaaaaaaata cagacatcat tagttaataa tgacatacct
     9181 ttttcagtta tggagaagga tggagtaaaa aaaatacaga cctattcctc ttttgatgaa
     9241 aatgatgtta gaaagcagat aagcagtgta agaaaggtgt ttccagatgc atttttatca
     9301 cacttagatg cacctatgtt gtctttagag tatacaagta actacgctta tatagagagt
     9361 atatctaaag aattaaataa attaataaca aactttaaag aagaatcaag tttttggtcg
     9421 aatgctgaaa ataatgtaga tatggaaaaa tataatacta ttttgacaaa tagaaaggca
     9481 atatcacaaa atatatcaaa agaagctgaa aagattgatt attctgaaat gcgtttattt
     9541 aaagataatt taatagagta tgtaaagaat gtgaatgaaa aaattgatac agcttcaaaa
     9601 gcagcaaatg aagaaaaata tagtgtaagt aaaagtctgc ttttatcatc catgcaggag
     9661 tactctatgt ttataaattc tataaaataa aatacagaaa atttggactt tagaaagcta
     9721 tatgaaaagg attgtttaat taagcaatcc ttttcctcaa tttgacttaa tatagaggta
     9781 ttgatttata aaataaatta taaaaatacg gatatttttt taacaattgt taagattttt
     9841 taatatatat agtatactta tagaaggcag aaaaaatgtt tttttataga taagggggta
     9901 aaacaatgaa actcttaact tttaagggag ggatacatcc tccatataga aaagagtact
     9961 ctaacacaaa ggcacttgaa aaagctcaag ctccaaagat agtttacatt cctcttcagc
    10021 aacatatagg ggcaccagct aaacctattg ttgaagttgg tgatgaagta aagtttgggc
    10081 aaaaaatagg tgaacaacaa ggttttgttt catgtaacgt tcattcttca gtatcaggta
    10141 aagttattgc tattgaacaa catgaagtac caggaggttc agctcaatgt gtagttatcg
    10201 aaaatgactt taaggaagaa ttacatgaga gtgttcaacc aaaaggtcag ttagaggatt
    10261 tgagcaaaga agatatagta ggtatcataa aagaggcagg aatagtaggt atgggtggag
    10321 ctacattccc taaccatgta aaagtatctc ctccaccaga tagcaaggca gaagtagtta
    10381 tattaaatgg tgcagaatgt gaaccatact taactgcaga ccacagatta atggttgaaa
    10441 atccagaaga tgttgtgttt ggtttaagag cattaatgaa ggtattagat gtcaaaaaag
    10501 gatttatagg aattgaaacg aataaaccag atgctataga agcaattcaa aatgtagcta
    10561 aagactatag cgaaatcgaa gttgttggcc ttcaagtgaa atatccacaa ggagcagaga
    10621 aacaacttat ttatgcgtgt acaggcaaag aagttccatc tggtggatta ccaatagcag
    10681 caggtgcagt tgttgacaac gttgccacag cagcacagat agctaagtct attaaaacag
    10741 gtatgccttt ggttgaaaga atcactacaa taacaggtag ttgcatcaaa gagccaaaga
    10801 acctaataac aaaagttgga acattagttt cagaaataat agaacaatgt ggtggattta
    10861 aagaagataa gaagattggt aaggttataa tgggtggacc tatgatggga atagctcaat
    10921 atactactga aatagcgact aacaaaggtt cttcaggaat tttatgtttg gatgaggaag
    10981 aatcacgtac tccagatatt caaaactgtt taagatgtgg aagatgtaca gatgtatgtc
    11041 catcattctt acaaccactt ttcataagtg catattcttt aaaagatgac tatgacacag
    11101 ctgaatatca tagagcaatg gattgtatag aatgtgggtc ttgctcattt atttgtccag
    11161 caagaagacc attacttcaa tctataagat cagcaaagag agaaatagga gcaaagagaa
    11221 gaaagcaagc tgctcagaaa taaagaaagt taagggggaa aaacgatgga aaataagttg
    11281 atagtatcat cttctcctca tgtgagaagt aatgaagata cttcatatat aatgaaacaa
    11341 gttattatag cactccttcc agcagcagta gcaggagtat acttctttag acttaatgct
    11401 ttgagtgcta tgtttttttg tatacttggt acagtaggta ctgaattttt atatcaaaaa
    11461 tttacgaagc aaaaaagtac tataggagat ttttcagctg ttgtaacagg attattatta
    11521 gcatttaacg taccagcatc acttccttgg tggatgtgtc tagtaggagg aatatttgca
    11581 atattagtag ttaaaatggt atttggtgga attggatgta actttgtcaa tccagcactt
    11641 gctgcaagag catttttatt agcatcattc ccagtagcaa tgactgcttg gactcaacca
    11701 ggtgttaact ggataggtaa aaatttagat gcagttacta cagcaactcc attaagcttt
    11761 ttgaaaaatg gagcagcagg actggcagac ctttctagca atggaattag tcttgctgac
    11821 atgatgatag ggaatattgg tggatgtata ggtgagacat cagcaatatt attattatta
    11881 ggtggagtat acctaatgta taaaggcata ataaattatg ttataccagt attttacata
    11941 gcaactgtat ttatattaac attcctttta ggtggattta atataacttt cgcaatatat
    12001 caactgtttg ctggaggact tatgttaggt ggattcttca tgcttacgga ttatacaact
    12061 tcgcctatga ctaaaaaagg tcaaataata tacgctgtat tagctggtct tataacaact
    12121 gttataagaa tgtatggtgg atatccagaa ggtgtatctt actcaatact attagtgaac
    12181 tgtcttgctc cacttataga taagtttgtt agaaatagag tatttgggga ggtggctaaa
    12241 taatgaacag tatggtgaga ttaggtggta ctttacttgc tataagtgca atagctgcat
    12301 tagctcttgg agctactaat caggttacag ctccagtaat agaacaaaga aatatacaag
    12361 caaataatga acttagaaaa gcagtccttc cagaagcaaa agaatttaaa gaaatgaagg
    12421 aagatactta caaaggcctt ggagatggtc ttatagctga agtgtatgaa ggtttagatg
    12481 gttctgaagt ggttggatat actttaaaag ctaaaccaag tggatatggt ggagaaattg
    12541 aagttatggt aggtatatca tctgaaggtc aagttacagg tgttgacatt ggtaatatgt
    12601 cagaaacagc aggtcttgga gcaaaagcta aggatgatgc atttaagggt caatacaaag
    12661 gaaaaactgc tgagcctctt gaagtagcaa aaggaagtac aacagcagat aatcaaatat
    12721 tagcaatatc aggtgcaact attacatcta cagcagttac tacaggagta aatgctgcta
    12781 tagatgtttt caatagtgct cttaataaat aaggagggtt aattatgaat ttagctaaag
    12841 tatttaaaaa tgggctaata gatgagaacc caacatttgt tcaagttata ggtatgtgtc
    12901 caacactagc cgttacaact tcagcaataa atggaattgg tatgggtctt tcaacagcag
    12961 cagttttaat atgtgcaaat ttagttatat ctttaataag aaaaattaca ccagataaga
    13021 taagaatacc tatatttata gtagttatag ctacatttgt tacaatagta ggtatgcttt
    13081 taaaagcata tgttcctgct cttgataagg ctcttggtat atatatacca ttaatagttg
    13141 ttaactgttt aatattagct cgtgcagaaa gttttgcttt caaaacagga gctatgcctt
    13201 caatagttga tggtgtaggt caaggtcttg gatttactgt tgcacttaca ataataggtg
    13261 cagtaagaga attacttgga aatggtagtt tatttggaat gacattattt ggagcatctt
    13321 tccaaccagt attaatattt atattaccac caggtgcatt cttaacatta ggattcttat
    13381 ttgcaggatt taataaatta agaagtaaaa aagcatagtg gaggtgtaat aaatgaattt
    13441 aatactttta tttttaagta tcgttcttgt taataacgtt ataacatctc aattcttggg
    13501 tatatgtcct ttcttgggag tttctaagaa ggttgataca gcagtaggga tgggagttgc
    13561 agttacgttc gttttaactc tagcttcaat tataacttat tttatacaga ttttattagt
    13621 aaaaactgga actggattct tacaaacaat agcttttata ttggttatag cttctatagt
    13681 tcagttcgtt gaaatggtaa ttcaaaagat gagtccatct ttatatcaag cattaggagt
    13741 ttatttacct cttataacta caaactgtgc agttcttggt atagctctag ttaatgttca
    13801 aaaaagttat aatttagtag aaacaataat aaacggattt ggagccggag ctggatttac
    13861 tttagcaata gtaatattcg caggtataag agaaagacta gaattagctg atataccaga
    13921 agcatttaaa ggtttcccta taacgcttat atcagcaggt ttaatgtcaa tagctttctt
    13981 aggatttaca ggacttataa agctataaaa aagaggggtg aaaatggtga tacttacagc
    14041 tgtattagtt ttaggaatta tgggcttaat cttcgggata gtactagatt ttgcatctaa
    14101 aaagtttgct gtagaagtag acgagagagt ggaagctata ctaggagtac ttccaggagc
    14161 caactgtgga ggttgtggat ttccaggttg tggaggtctt gcaaatgcga tagttgaagg
    14221 caatgcacca gttaatggtt gccctgtagg tggagcagat gttggtgcaa aagttggaga
    14281 aataatggga ataagtgctg aagcaggtga gaaacaagta gcaaaagtta tttgtaaagg
    14341 aacttgttca agtgcaaaag ataaatatga gtatgaagga atatcagatt gtagagcagc
    14401 aaatgtttta aactcaggtg ctaagatgtg taaatttgga tgtttaggtc ttggaacatg
    14461 taaagatgct tgtaaatttg atgctatttc tatagttgat ggaatagcag taatagatga
    14521 agaaaagtgt gtaaattgtg gaaaatgtaa agaagtttgt cctaagggta taataatcac
    14581 aaaaccagaa agtcaagaag tagttgtaga gtgtaatagc aaagagtttg gtaaagctgt
    14641 taaggaaaaa tgtactgctg gatgtattgg ctgtggaatg tgtgtaaaag cttgtaaatt
    14701 tgatgcaata atatttgaag acaaaatagc taagatagac cctaataagt gtgtaggttg
    14761 tatgcaatgt gtagcaaaat gccctacaaa agtaatatca ggtgacataa ctaagaaaaa
    14821 gaaagttact attgaccaag aactttgtgt tggatgtaca gtgtgcaaaa aacaatgtaa
    14881 gtttgatgca atagagggag aattaaaaga aaaacataag gtagatgcag ataaatgtgt
    14941 aggatgccat ttatgtatgg aaaaatgtcc aaagaaagct attaaaattt tgtaatatgg
    15001 acaaaataag gatatggaat aatttccata tctctaatga gtacataaaa cagaggtatg
    15061 gaataatttc catacctcta ttttatgtat atttattcta atggtaataa atataacaat
    15121 agtgtaacaa aaaaggttcg acatttctgt tgaatttttt acaatatagt gatagactaa
    15181 ataggtacaa attacgtaca aatgaggtga ataaatgaat ataatcttgg catcagcctc
    15241 tccaagaagg aaagaaattc tagagaatac gaatgtaaga tttgatataa ttaaaaatga
    15301 aattgatgag attatattgg aaggtgaaac accaaaacat ttagttatga gattagcttt
    15361 tgaaaaaagt atgtctgtag catctgaaca taatgaagat gttgttattg gagctgatac
    15421 agttgttgtt ttagacaatg ctattttagg aaaaccaaaa gatgagtctt gtgcaagaga
    15481 tatgttaaaa agactatctg gaagagagca tcaagtcata acaggtatta gcttaataaa
    15541 cttgtgcgaa gataaaaagg taattgacta tgtaatcagc aatgtgaaat ttaaaacgtt
    15601 atcagagcag gatattgagg attatctgaa aactaatgag tcatttgaca aagcaggagc
    15661 ctatggtata cagggttatg gagctttgtt agttgaagaa attagaggag attattttaa
    15721 tatagttggt cttccaattt caaggttagg cgacctatta aaaaaatatt ttagtataaa
    15781 tcttttttat ggagtgtgat ttatctgaaa aagtcattta attcaaccat taaggtaaaa
    15841 gaaatggcac cagaggaaag acccagagaa aagatgcttg ccaaaggcgt aaaaagctta
    15901 tcaaatgctg agttgttggc tatactttta agaactggca acaagaataa aaatgctatt
    15961 gaattagcca actatataat aaatagagat attcagggta ttagacattt agaagatatg
    16021 acaatagagg agctgtgtaa tatagatgga attgggttat caaaatcaac ccaaattaaa
    16081 gcagctttag aactaggctc tagagtagct agttttaaac ctataaagta taaaataagg
    16141 aatccttggg atatacagag gtattatatg gatagcctaa ggtatttaaa aaaagaggtt
    16201 tttaaagcgg ttcttttaaa cacaaaaaat gagataatat ctgatgtaga tgtatctata
    16261 gggactttaa gctcgtcatt agtccatcca agagaggttt ttaaagaagc tataagaaga
    16321 agtgcaagta agataatagt gatgcataat cacccttcag gaagtgtaga accatcaaga
    16381 gaagataaaa atatcacatc aagacttatt aaatgtggag aaataattgg aatagaaata
    16441 atagaccata taattattgg agatggatta tactttagct ttaaagaaaa tatgataatt
    16501 tgactttata tgtgataaaa taataggaga atgttttaaa catgattctt aatagtagga
    16561 aggagtaaaa tcatggctaa ggaaaaaaag aaagaaaaaa aaggtttttt tagttttaat
    16621 aagatgacaa aggatatggg aatagattta gggactgcaa atacattagt ttacataaaa
    16681 ggacaaggta ttgttgttag agaaccatca gttgtcgcaa taagagatga tagtaaagag
    16741 gtattagcag taggtgaaga agccaaaaaa atgataggta gaacaccagg aaatatagta
    16801 gctataagac ctatgaaaga tggagtaata gctgattttg acataactca atcaatgatt
    16861 agctatttta tacaaaaagc ggctgataaa aaaggtgttg taagtccaag aatagctata
    16921 tgtgttcctt ttggagttac agaagtagaa aagagagcta tagaagaggc tgcaagacaa
    16981 gcaggagcta aagatgcatt ccttatagaa gagccaatgg cagcggctat aggggctgga
    17041 ttaaaagttg aagaaccaga aggtaatatg gtagttgata ttggtggagg tacttcagaa
    17101 atagctgtaa tatctttagg aggaatcgtt actgctaaat ctataagaat aggtggagac
    17161 gaatttgacg agtctatagt ggcttatgta aaaaaagaat ataatcttat gataggtgaa
    17221 agaactgctg aaaatgtaaa aattaacata ggttctacat ttaaagatga tgaagaaata
    17281 aatatgcaaa taagaggtag agatttaatc tcaggattac ctaaaacaat agaaatatgc
    17341 tctacagaag ttagagaagc attaaaagaa cctgtaagtt caatagtaga tgcaataaaa
    17401 tctacactag aaagaactcc accagaatta gcctcagata taatggaaaa cggaataatg
    17461 ctaacaggag gaggcgcttt gcttagaggt cttgataagc ttattacaca ggaaactggt
    17521 atgtctgtac aaatagcaga aacacctctt gattgtgttg cacttggaac aggtaaatct
    17581 gttgaagacc aagaaatctt tgaaaaagta ttaatgatga atactaagaa ttagaagttg
    17641 gtgatggcct tgagatttga caagaacaaa aatgagaaaa aaaggattaa tgttaaagtg
    17701 atagcaacgg gcgtagttgc tatcacttta attggaattg tgggtatatc aattggtaaa
    17761 ttttctagtg gtagtccagt aaatttagga gtagcttcag atgctattac aagtgtaggt
    17821 aagggaataa atgatggatt ttcttttatc aaaaatggtt ttaaagatgt agcaaatttt
    17881 aaagataatt ctaaaaaggt aaaaaaatta gaagaagaaa atgaaaaact taaaaaaaat
    17941 atgatagctt taaatgccaa actagataaa acagaatcgc tagaagagtt aaaaaagaca
    18001 ctgaactttg tacaagaaga gtataaggcg acaagtattt cagcaagtgt tgttggtaaa
    18061 aatgatggaa actggtatga gagttttgtc ataggtgcag gaaaaaattc aggtgtaaaa
    18121 aaagaaagta tagttatgaa tggtagtgga ttagttggca tagtttatga agtttccaac
    18181 aattatagta aggcaatttc actattggat tctaaggcat ctgtaagctt taaactagca
    18241 aaggatgcca atgctaaagg tacaattact caaaatacta ctttagataa taaagatagt
    18301 tataatagta aaggatattt gcaaggatat atgtttgact catcatataa tgtaattcaa
    18361 ggagatataa taacaacatc aggtcttgga ttcttcccag atggtatacc aattggcgaa
    18421 gttgaaaaag tggttgatga taaagataag tcattaaaat atgtagttgt aaaaccttat
    18481 gttgatttta agaatataaa tgacgttgta gtcatagaac caagaaatat aggataaagg
    18541 ggataaaaac aatgaaaaaa gttttacttt gtctgttggg gattgtgctt atgactatag
    18601 aaaatagtat aacaaattat atagatattt ttggcataag ctttaattta gttcttatat
    18661 atgtaaccat aatctctctt tatttagatg aattagaagg tgggattata gcagcaataa
    18721 tagggcttat taaagacata accgttggtg gaatttttgg tgtaaatgca ttaattttgt
    18781 ttattattgc atatgcaatt ggatacatga gagatcagct atataaaggt agttatatta
    18841 ttactttcgt tttaattttt ataggcagtt taattgattc aattgtcaat ataggtacat
    18901 ctagtataat ttatcaaagc tacaacatat caacactttt tgtaaaaggt ctactagtag
    18961 caccaatagt gaatagttta attggtttag taatatatcg cttatctaaa agagcagttt
    19021 taaaacttaa agaagattag ttggtgattt catggaagaa aataaacaag ttgatagact
    19081 aaaaataatt aagatagtca tgggcgtgat atttttagct attttagtaa aaataatcta
    19141 tatgactaca tttaaatatg aatattacaa tgagttagct gagaataaaa catataaaaa
    19201 gttggcaata gaagctccaa gaggagaaat aaaagataga tatggaagac tgttggcagg
    19261 aaataaaaac ttatttactg ttcaagtttc aggtaacgat attaataaga aagatgcaaa
    19321 taagcattca agagcaaatg aaatatcatt aaaacttata aatttgctgg agagaaatgg
    19381 tgaagaatat gtagatgagt ttcctatata tgtagaaaat ggaaagtact attatacata
    19441 tgacagagat attagggaat ataaatcaga aaatggtatt ccaaatgatt ataatgctaa
    19501 ggaaagtttt tattacttgg tggacaaact tatatcagct ggaatattat ctcaagaaga
    19561 taaaagattg gatgcgacaa gattacaggc aaaactaaat gaaaatggat attatccacc
    19621 tattttagtt agtaaatgga tgttcacagc agaaagagat aagagagatt ggttggctag
    19681 ttataagata aaagaaacta aactgagtgc caaagaagcg tttgaaaaag taagaaatag
    19741 tgatgcatta gaaattgata aaagtttgag tgatgaagat gcaagaaaaa taatggtcgt
    19801 aagggatttg ataaaatcaa aaggatattc tcaatataat ccagtaacta ttgctaagga
    19861 tgtaggagaa actacgattg ctcaaattga agaaagtgct atggaccttg ttggtgtttc
    19921 aatagcagta gaaccagtaa gatattatcc taatgggtca ttagcttctc atatgttagg
    19981 atatgtagga aaaatgccat ctactcaaat agaatcatac cttcaaaaag gttatgaaac
    20041 tggagatatg gtaggtcttg caggagtaga aaagagtaat gaaagtagat taagaggaac
    20101 tgatggttat aaaatggtta aagttgatgc attgggtaga attagtaaag aaattgaatc
    20161 taaaaaacct aaatcaggag atactgtgta tcttacacta gataaagatt tacaagaagt
    20221 ttcagataat gcattaaaac agataatcga agttgcttct aaaggaggta cttttaaaag
    20281 taaatttggg gacaaaccaa ttagtgcata tgcaggaaaa gcacagtcag cagctcttat
    20341 agctattgat gttaaaaatg gagaagtatt agcttcatca agttatccta attatgaccc
    20401 aaataaattt gcaaaaggta tttctacaga ggattataaa gcacttcaac caaaaaatcc
    20461 aaatgactta ctagcaggaa gtccactttt aaacttggtt acacaagggg aatttcagcc
    20521 aggttcttca tttaagatgg taacttcaat ggcagcgtta gaaaatggtc tggatccaaa
    20581 ctttactata aatgaccctg gggttataat gttaggtaaa aaatcttttg gtgactatgt
    20641 atggaaccat ggtagaggaa accatggtat gacaaactta tataaagcaa tacaagagtc
    20701 ttgtaatata tatatggcaa caattggtac aggtaaaaca tggccagatg gaaaaagcat
    20761 tggtatagat atgaatgcaa ataaaatact tgagtatgca aaattatttg ggcttgacca
    20821 aaatacaggg cttcaagatg aagtagaaga aagagcaggt aaagttccta gcacagaaga
    20881 taaattgaaa tcaacacaag ctcttctaaa gtcaaattta gaaagagaaa tggcaaatga
    20941 ttttgttgat ataactagag aaaaaaatcc taaagaatat gagaaaagaa tcaatgagat
    21001 tgtaagctgg gcagcagaaa agaaaactcc tggtagagtt gaaacaatga ataggctaaa
    21061 aaaattggat gttaaagaag atagaattga agaagtagca gatttagcag tatttagtta
    21121 ttttaacttt gcaaaatgga gtactgcaga tacatttaac ttagctatag gtcaaggtga
    21181 aaatgcttat acaccagctc aaatatcaag atatgtagct gctatagcaa atggaggtaa
    21241 tttagtagaa ttatctgtag ttgatagagc tgtttcaagt gattattcat cagttaaaat
    21301 taatgaccag aagaaagtcg aaaaaatacc ttttaaaaat ccagataact taaaagaatt
    21361 aacaaaaggt atgaaattgg ttgcaagaca aggtacagcg aagagtgctt ttgctgattt
    21421 tccaatagat gtagcagcaa aaactggtac agctgagaaa agtgggaaaa tacctacaga
    21481 taacgagtat gaatatttaa aaagtcatat gtcatcttat aatgtcaacc taaatgatgc
    21541 gattaaacta gctgataaga tgaaagcaga aaaagaaaaa gagctttcat tagctaagga
    21601 aaaggaaata aaaaagaaat tagaaaataa agatttgaaa gacgaagaaa gaaagaaatt
    21661 agaggaagaa ttagaagatg gagtaaaagt tagattagag gacactgata aggtgaattc
    21721 ttcatatcta agaaaagcta taaaagaatt aaatccaaaa ataacagatg accaaataga
    21781 tagatttaaa caagattatg gttcttttac gtggactgtt gcttttgctc cagcagatga
    21841 cccagaaata gctgttgtat gtgtaattcc tcaaggtgat tcaagtgtat tctcactttt
    21901 accaactaga gaagttattg gaacttatat gggattagag ccaactaata gtaaaaatga
    21961 caacaagacg aatgatgtta acaattcttc tgacgaaaat ataaatttcg agtcacaaat
    22021 taatagatag ctcaatctat gtataaactt tagctttata tagaaaaaat aaaagggttt
    22081 aaagcgtatt tgaagaatat aaatgatata tcactctgga ggttgttatg tctttaagag
    22141 aaatttgtag tcaagaacta gtagagttca agggtaataa aagagggata atagtaaata
    22201 taaaaagaga agctccattt gaggaaatac aggaaaaaat aataaacaaa ttggaagcat
    22261 atgttggctt ttttaatggg gctaaaatta gtaaaattaa tagtgattgt ttaactgaca
    22321 tggagattct agaacttaaa gaaggaatta cttctagatt tgatgtggaa tttgttgaag
    22381 atcaaaaaat tgaagaaaat agtaattttc caacaaaata tgtcaatacc ctaagatctg
    22441 gtgaaaatat tgaatttgaa ggtgatgtag ttatattgaa tgatatgaaa cctggttcaa
    22501 aagtcctttc aaaatcaaat acagttgtta tgggagatat aaatgctggt gctaaggttg
    22561 tagcaggagg taatgttttt gtaatgggta aaatagaagg ttttgtacat gcaggtgcag
    22621 aaggcaatga atttgcatat gttgttgctg gaaatcttaa tcctaaaata ttacaaatag
    22681 cagataatat tgcagaagct ccagatgatg aagaaaatta tgaaagtgaa tcagaaatta
    22741 gcccagaaat agcctttgta agtaatggaa gaattgtaat cgaaagctat ttgtcaaagt
    22801 tagacaaata gaaagaaaaa tagggggtag tattatgagc gaagttatag ttataacatc
    22861 tggtaaaggt ggggttggaa aaactactac cgcagcaaac ttaggaactg cgctaagcct
    22921 agaaaataaa aaaacagtag ttgtggatgc agatattgga cttagaaact tagatgtagt
    22981 aatgggtctt gaaaatcgaa tagtttatga catagtagat gtagttgaag gaacttgtag
    23041 gcttaagcaa gctctaataa aagacaaaag atttgataat ctatatttat taccagcagc
    23101 acaaactaga gataaaaatg ctgtctcagt tgagcagatg attgacctgt gtgagaaact
    23161 aaaagaatct ttcgagtata taataataga ttgtccagca ggtattgagc aaggctttaa
    23221 aaatgcagta gctggggcag atagagctat tgtagttact aatccagaaa tatcagcagt
    23281 aagagatgct gatagaataa taggtctatt agaagcaaat gaaataaaag agataagatt
    23341 agttataaat agaattagaa atgatatggt taagcgtggg gacatgatgg ataaacaaga
    23401 tataatagaa atattagcaa tagatttgtt aggtcttgtt cctgatgatg aaagcataat
    23461 tatatcaaca aataaaggag agccagctat acttgattct aagtcacttg ctggtcaagc
    23521 atacaaaaat atcgcaaaaa ggatactaaa tgaagaagtt cctctacttg atcttgaagt
    23581 tgaagatgga ttctttggta gacttaaaaa aatgtttagc atggctaagt agcaaaggag
    23641 gatacatctg tgttagattt atttagagtt ttttctaacg aggctaaaac tagtaaatct
    23701 gttgctaaag agaggttaaa gctagtttta gttcatgata gagtagattg ttcaccacaa
    23761 cttttagaga tgataaaaac agacattttg aaagtaatag ctaattatgc agaaatagaa
    23821 gatgatggtc ttgaaataaa aatgtctaaa tgcagaggtg agcatgatga caaacctgta
    23881 tctgcattag ttgctaatat accactaaag aatataaaag atagatgtat gtaattagtg
    23941 cttaaaaggg ggatatcctt attggtatat gcccctttta tttaattata tgtttaaaga
    24001 tagaaagacg gtgattcttt gaaaaaaatc aatattaatt ataagtcgac aattaagttg
    24061 ataaaacagt tagactggaa acttatagtt acagtattag ctatctttat ttttgggctt
    24121 gttatactta gtagtgcaac acatgctaat tcaacaggtt catataatca attgattaaa
    24181 caaggattag cttttgtact tggtatagga atgattatag tcatattatt ttttgactat
    24241 aaccttctag gcaggtatta taaagcgctt tatataataa gtcttatttt gttagccata
    24301 gttttattac ctggaattgg gacagtaaaa ggtggagcta ggtcgtggat taacttagga
    24361 ccacttgact tgcagacatc tgaaatagtc aaattaacat ttgtactaag ttatgcaaag
    24421 atacttgaat ccaaaaagga taaattaaat acgttaaagg aagttatgcc tgttgtagtt
    24481 tattctcttc catttatcgg attattgatt gcacaaccag acttgggaac aggaattgta
    24541 ttttgttgta tgatatttgc aatgctgttc acagcaggtc ttagctcaaa gttgataaaa
    24601 agaggaataa tcatactatt agtgtcaatg ccactgatgt atttaatgat ggcagaccat
    24661 caaaaggtaa gaatagaagc ttttctaaat ccagaagatg taactctaaa aggaaactat
    24721 caagttatgc aatctctgat tgcaattggt tctggaggcg taactggtaa aggactttac
    24781 aatggaagtc aaaatcaaga agatttttta cctgtacaag atagtgattt tatatttgct
    24841 gttgtaggtg aagaacttgg tgtaatagga atggcagtgt tgataatctt atttatgata
    24901 ttcctattaa ggcttttggc aattgcaaga gatgcaaaag atttttatgg aaccctcata
    24961 gtggttggtg ttatgggaat gtttggttac cagataatcc aaaatatagg tatgacggtt
    25021 gcgttaatac cagtaactgg agtaacattg ccttttgtaa gttatggagg aagttcgtta
    25081 ttgacatctt tagcaaactt aggattagta ctaaatgtgt gtatgagaag aaagaaaata
    25141 aatttctaaa tttaaataca tctaaacata aattgaggag attagatata tgaatatagc
    25201 attagtagca catgaccaaa tgaaaaacac tatggtcgga ttttgtatag gttatgaatc
    25261 aattttaaaa aagtatggac tatatgcaac tggaactaca ggaaaaagaa taatggatga
    25321 aactgaatta aatataaata gattagcatc tggtccttta ggtggagacc aacaaatagg
    25381 ttctttaata gtaacacaag aaatagattt agttattttt ttaagagacc cattgacatc
    25441 tcaggctcat gaaacagata tacaagcgtt aataagactt tgtgatgttt atcatgttcc
    25501 aatagctaca aatctagctt cagcagaaat atttataaaa gctcttgata gaggagaact
    25561 atcatggaga gaagttagaa aaagcaaaag tcaacgtatt taaaaaatat taatattaca
    25621 gtctaaagaa acagatatat aagtgtttaa tgccctagtc tttctgttgc aataaaccta
    25681 gtacctgtag aaatcttatg aaagatttaa atgatgaaga gctgaatttg agattaaata
    25741 attaggaaat agattataga tagagtagta gaatttaaac tattactcta ttttaataat
    25801 atgaaataga atcaagtcta ttattatata tatttaaact tataggtaat actatttcaa
    25861 tcaagtaaat gttgttatga caacattaat aaaataaaaa cttattttta aaatttgata
    25921 aatagctgta tattagtatt tatagattaa atattaatat acagctattt attattttta
    25981 agtctattgt agaatataaa ccttgcaata tagagtacta tgtaatatta tagtaaatat
    26041 aagtactata cagtatagag tagtaggtga aaaggcggtg gtacataatg cagttaaata
    26101 aagaagtgtt aaaaggttat atcgatatat taattgtttc tatattagaa aagaaagatt
    26161 gttatggata tgaaattgca aagcaagtaa gagaaagaag tgagtttgaa ttaaaagaag
    26221 gaacaatgta tcttgcactt aaaagaatgg aatctaaaaa tttaattaaa tcttattata
    26281 gcaatgaaca aagttcaggt ggaagaagaa aatattataa tttaactaat gaaggtaaag
    26341 attttctaga aataaagaaa caagaatgga gatttattaa aaaggttatg aatcaatttt
    26401 tgggagaggt ggattaattt atgaaactaa tagatgaata tttagataaa ctatataaaa
    26461 aatgtgacaa taaaagtact atagaattaa aacaggaaat gagatgccat cttatagaat
    26521 ctgcaaatga atttaaatta gaagggttag atgaagaaga agcatgtaaa aaagcaattg
    26581 aaagatttga tgatggtgat gaaatgcagt atgaattatg taatataata aaagaactat
    26641 ctttatcatt ggataggcat aaatccattg taatggggtt taaaaaagta ttaggttaca
    26701 taagtataat tgcattttta atttctggat ttatgtggta ttacaataat agtttacaac
    26761 ataatatgta taatttagga aaagagcttg atggagaaat aaagcaatta gcagaaagac
    26821 atgatatgac aaagattggt gaatataagt tagaacttga aaaaatactt gataaagata
    26881 agtatagtaa agtcaaagct cttagactat atgtaataga tatgaaagat ggaaacacta
    26941 atttaagctc ttctggattg aatgctaata tggtgtatga gagtgaagca gattataata
    27001 acataagtaa ttttatacag catttaggat ataatggaaa ggatttttta gacaagaatg
    27061 ggaatatagt aaatccagat attttcttag aatatttctt ttattttgaa tcggagatgt
    27121 taattccagt agcattcgta tttggattat tatgtataat agcgtatttt atattaagat
    27181 ttaaaatttc tttaataaaa aataataatt aaataattca ccaaataatt ttaatttgta
    27241 gagtaaataa gatgtttcaa aataaaattt gaatcatctt attttttatt gtttgttata
    27301 ctcttttctt ttatatttct ccatagtgga ttgtatctat acataatcac atattttgat
    27361 ataattatac tttccagaaa atgtattgaa tatacaaatt ataatctgta taatgttgtt
    27421 aggggagaat gtatgaagtg tatacaattt taattttaaa tgtattattg acaattctcc
    27481 tcactctcgc cattaccaat aattggaaaa ttgacatacc tgttacagac caacattttt
    27541 gaatatatta attattatca ttttattgtg atgatatcaa aaatgcaggt ttgtgagtta
    27601 gattgtatac gaactatata ggaaatatag aacagatttt ccaagaaatc taaatattct
    27661 atgacaacgt gaattgctat gcatacatgt tgataatcca tattttaaat ctaaaatgaa
    27721 cttaattaat ggtagggaat tgaaagtaaa atatattata aaggagaatg ctaatatgaa
    27781 aaaaatcagg aaattagcta ctaaactatt gattgcaatg atggttttaa cctcatgtat
    27841 gagtgtaaca gtttatggta tgacagctaa agatgttact gcaaagacac cgaaaagcta
    27901 tgtagctgga accaacagta tatatggtcc aaagttatca caagctcaac ttaattcggt
    27961 agcacaagca acagcagact ttatgaacaa gaaaattaca aagaacatga ccacagatgc
    28021 taagattagg gttgcttata atcacattaa aaacaacaca acatatgtta actggaacgc
    28081 agcagaggga gccaatactg cttatacatt ggttactaaa aaaggtgctt gttctggaat
    28141 gtgtaggagc ttgaaagctt tatgtgatgc tatgggaatt gaatgttatt atgtacatag
    28201 tacaacaaat gaccatcagt ggaatcttat tcgttttgag gatggacgtt tgtatcatgt
    28261 agatattgat gccaacaaat cagcaggagc agacatcata tacaagagtc ttactcaccc
    28321 attaccattt gacaagaaag catatccagc tgttggcgaa gtagtaaaga cacagaatcc
    28381 aacgccaact gttaagaaac ctacgccagc taagccttct gcgccagttg gacctgggaa
    28441 tcacactatg atagcagaac aacaaggggc aatacttgat gaagtttatg gaaataatct
    28501 acgtccaaaa attgaaatgt atgagggagt tccgtgtagt ttaataaact catcaataaa
    28561 tacaattaag tttacaagca aaaattcaaa tttagaccca tcaaagcata cagtttctgt
    28621 aatagggttt atgaatacac catatggtat ggttgcatta gctccagatg gtgtacctgt
    28681 tgagtatcct tttgaatatg ataagccatt ttctttagat tttactggta agaacctttc
    28741 tgcagcagaa ttatcaaaaa agtgtaaaga aaataatgta attattaaag tagaaattgg
    28801 tgaaacaaca tctaattctg gaatagggct agtagcatat gctagataca tgaattaata
    28861 caagtatata aatagaaaag attataaata atgtaccctc ttggtgaggg tgcatttatc
    28921 attatattag tcattttggg aaataaaaaa ggagttgaac ttaaaattag attagaaaaa
    28981 ttaattttga gacaactcca ttttaattta tttttaaata taaaatcatt tattttaaat
    29041 aatattatgt ttgattaatg gctatttaac taattttgca aattctgcac caaatgccat
    29101 acactctttt aatgtctctt cactagggaa gaattttttc ttaagagatt ctacaggcat
    29161 tttgaatgac atagatttaa gtaaagtctc agccatagtt ataccttcac cactccaacc
    29221 aaatgaacca aatactcctg ctatcttgcc ttggttagcc attgggtcta taacagagaa
    29281 taaatcccac ataggtttaa ccatagtttt gtttatagtt ggagaaccta gtaatattac
    29341 ttttgacact actaatgtat cgtgcatttc tgttaatgtc atatcttcta aatcatataa
    29401 ttcagccttt gcaccttctt tatcaagtcc ttcttttatc tttttagcca tctctaaagt
    29461 attactataa gctgataagt agaatataga aacttgattt tgattagttg tattaacagc
    29521 ttctgtagac cactcaacat atcttttaac agcagccatt gggtctttag taagcattgg
    29581 accatgagaa gtcaatatag tatcaaattc tatgtttagt cctaccacct tatctacagc
    29641 acttaaaaca tgtttagcaa atggcttaac tatacaatca tagtagtgtt tagctgattt
    29701 tagataatct tcactattca ctacctctgc atcaacactt gcaaagtgac aaccaaatgc
    29761 atcacaagtt aaaagagtct tgtcctcctc aatgtaagta aacattgtat caacccaatg
    29821 taagaatgga gcagttataa atctaagatt tcttttaccg atatttaaaa tttcaccatc
    29881 ttttattaca tgacagttaa aaggtctatt tatttgacca tctaagtata gtttagcagc
    29941 tttagtacaa taaacttcta tattaggatt tatatctaat aagtatttta aactacctgc
    30001 atggtctggt tctgtgtgat gaattacaac atagtcaatt ttagctatat cagtaacctc
    30061 tgataaatta cttaaaaact tatctttaaa atttgccttt acagtgtcaa ataaaaccgt
    30121 tttttcgtcc ttgattaaat aagagttgta agttgtccca aactcagttt ccattattat
    30181 gtcgaatact tttaaacctt catctactac gccagtaaag tagatatctt tttttacttc
    30241 aaaaacttta ctcattttta accctcctaa aatttatcat tatataagta acatacccca
    30301 ttttgaggtg aatgaaacaa aagatgtcat gataagtcat gtaaaacaat agttttatgt
    30361 ttataatata tcatactttg taaaaaaatt gtatttcaat aaaaatttaa tagaaaatat
    30421 tcttaaatat ttttagctat tctattttgt gaataatatg tattttaaat tcaatatttt
    30481 agttatattt ttgatagaaa atattttatc gtgtataata ttacttataa ataagaataa
    30541 aaataaagag cttgtttgga ggaattttaa taaatgaata aagtagactt gaaaaaaatc
    30601 ttaaaaaaag tagaaaagcc tgctagatat cttggaaatg aaataaattc aattcataaa
    30661 gatactagta atagtgaatt gataagatat gcacattgct ttccagattt atatgaagtt
    30721 ggtatgagtc acttagggag tcatatatta tatgatgtaa taaataagga tgaagatgta
    30781 ttttgtgaga gagtgtattc tcctgcagtt gatatggaaa atataatgag agaaaaaagt
    30841 ataccattat ttgcattaga gtcaagagag cctataacaa actttgattt tgtcactttt
    30901 acacttcaat atgaactttc atacactaat atcttaaata tattagattt agccaatata
    30961 ccaatattaa aagaagaaag aactttggag gacccattta taatggttgg tggtccttgt
    31021 gcttataatt ccgaaccatt agtagatttt gtagatgtag taatattagg tgaaggtgaa
    31081 gaggttaatt tagaagtagt aaatgagtat aaagaatgga agaaaaataa aacgactaga
    31141 gaagattttt tatataaaat atctagcata gaaggagtat acataccaag tttttatgat
    31201 gtcaagtaca atgaagatgg tacagttcaa agtgtaactc ctaatagaga cgggattact
    31261 aaaaatccta ctaaaaggat aataaaagat gttgagactg tagattatcc agaaaaacta
    31321 atagtacctt atatagatac tgttcatgat agaatagtac tagaattatt tagaggatgt
    31381 actagaggat gtagattctg tcaagcaggt atgatataca ggccaataag agaaaagagt
    31441 gttaaaagac tgaaagaaat attagataaa ttagttaaga atactggata tgatgaaata
    31501 tcattatcat cacttagtac gagtgattat agtaagttat cagagttgac tgattatttg
    31561 gtagatgaat atgcatctaa taatattggg atttcgcttc catcccttag attagataat
    31621 ttttcaatgg aaattgctga taaaatacaa caagtaagaa aatctggact tacttttgct
    31681 ccagaagcag gaactcaaag acttagagat gttataaata aaggtgttac agaagaagat
    31741 ttggaaaatg caacagaaag agcttttgag atggggtgga atagtgttaa gttgtatttt
    31801 atgattggtt taccaactga gacttatgaa gatttagatg gtatagctaa attagcttat
    31861 aaagttattg atatttatag aaaagtaaat ggaggaaagc taaagagaag ttttagtgtt
    31921 acagtgagta cttctacatt tgtaccaaag ccatttacac ctttccaatg gcatggtcaa
    31981 gatactacag aagaagttat taataagcag agacatttag taaataaatt aagaaataac
    32041 aatataaaat acaattatca tgattcaaag actagtttga tggaagcagt agtagctaga
    32101 ggcgatagaa gaataggaaa agttatatat gatgcattta gactaggtgc taaatttgat
    32161 ggatgggcag aacattttaa tttagatata tggaaagaag ctatggaaaa gaataatctt
    32221 tctatagatt tttatgcaca tagaaataga aactatgaag aggtatttcc ttgggaccat
    32281 atagatgtag gtattagtaa aaaattctta attagagaag acgaaaatgc taaaaaagaa
    32341 aaaatcactt ctgattgtag acataactgc aatggttgtg gaataaatat acatgatata
    32401 ggaagggggc tttgctaata tgagtaagat aataagagta aaatttaaaa aggaaggtga
    32461 catgatatat atatcacatc ttgaccttca gagattgtta caaagagcgt ttagaagagc
    32521 tgaaataaac ttatcacatt ctcaaggatt taatcctcac cctaaaatga gttatggcaa
    32581 tgctttggct ttagggacag aaagtcaagg tgagtatgta gatattgaaa tagaagaaga
    32641 tgatttatca gtagaggaat ttttaaataa agtatcaatt caacttccag atgggataga
    32701 ttttatcaaa gcaaaagaaa ttgatagaca aacaccttct ctttcttctg tgatagacta
    32761 tggagaatat ttgtttaata tagatttgaa aagacctatg acgaaagagt ttgtaaaaag
    32821 aaaagtaatt gactttatga ataataaaga aataatcata acaaaaaaga ataaaaaagg
    32881 taagatggtt gaagtagata taagacctat gataagaacc tttgatgttt taaatttaga
    32941 agatgaacat attactctta ctgctactat agctacaggc tcaaaaacta atttaaatac
    33001 taatatactt ataccaaaga tattagagat gtttgaactt gatattgacc cattagatgt
    33061 tgatatattg agaagagatt tatatgtatt agaagatggg gaattagtga ctcctatgta
    33121 agctcgagag gtgaagttgt gaaaaagata gttattgagt ccctaatagg gtcccagaaa
    33181 acagcagtac ttgaagatga aagattaaca gaactttttg ttgaagacaa tttaaataag
    33241 aagactgtat ccaatatata tcgtggaatc gtaaaaaaag ttataccagg aatagaagct
    33301 tgttttgtag atattggatt taagaaactt gcttatttac aattaaaaaa gggaagtact
    33361 ataaaatctg gtcaggatat tttggttcag ataaataaag aagaaatagg aactaaaggc
    33421 gcaaaattaa acactgagat tagtatttct ggaaggtata ttgtctatat accttccaat
    33481 gatagaacta caatatcaaa taaaattact gatgaaaaag aacgatttag attaaaaaaa
    33541 attactaagg cagtaaataa ggaaaatcta ggattaataa taaggactga ggctcaaggt
    33601 tgcactcatg atgaaataaa gaaagatata gaagagttga aattaaagta tgaaaacatt
    33661 ttaaaagaat ataaacttgg tataggtccg aaattattat ataagtcttt agattttgca
    33721 actaaatatg taaaagataa tgtgaatgat gatattgaat ctataattac caatagttat
    33781 gataagtact ctgaacttaa gtctatttta agaggtatag ataagactta tgttgataaa
    33841 ctgtgtcttg aggaaaatag agatgtattt gatttataca gaatagaatc taaaatagaa
    33901 aaattattaa ataaaaaagt atggttaaag agtggaggtt atttaataat tgaaaaaaca
    33961 gaagctctta cggttataga tgtaaatacg ggtaagttta ttggaactgg taaacttgat
    34021 gaaaccgttt ataaaactaa tcttgaagct gcaaaagaaa ttgtaagaca gcttagaatt
    34081 agagacatag ctgggataat aataatcgat tttattgata tgcataaaaa gaagcatcaa
    34141 aaggagattt tacacatcct agaagaagaa tttaataaag ataaaagaaa agctgaagta
    34201 ttaggtatga caaaacttgg tttagtagaa gttgctaggc gtagagaaaa agaatccatt
    34261 gataagtatt atttaatgtc ttgtccatgt tgtgatggtg aacaaacaat aaaatctgta
    34321 cattatatac tagatagtat tgaaaaagaa attatgagaa taagtgaaca tactgtttac
    34381 aaaaacataa tggtagaatt taatgatttt atttttgaac aaataaaaga atattatatg
    34441 gatataattg ataaaattgg tgaaaaatat aatataaaga tttctttaaa cgcaaatagt
    34501 acattaaagc acaataaaac caatgttatt ttcgataaaa tagttgacaa taaaatgtaa
    34561 atattttata attaataatt gtaatgccgc acagtaaagg tttaaatctt caaatgaagt
    34621 accttgtact ggcgagtttg aggccgagga ggtgtattta atgtacgcta tagtaaagac
    34681 tggtggaaag caatataaag tttctgaagg tgatgtatta ttcgttgaaa aattagaagc
    34741 taacgcaggt gatgttgtta ctttaaacga agtattagct tgctctaaag atggagaatt
    34801 aaaattaggt tctccagtag tagaaggagc atctgttcaa gctaaagttg ttgaacaagg
    34861 taaagctaag aaagttatag ttttcaagta taaagcaaaa aaagattaca gaagaaaaca
    34921 aggacatcgt caatcataca ctaagatagt tgtagaaaaa ataaacgctt agttattggt
    34981 taagttatga taaaagttaa atattattat aatgatgatt tcttgttgca aggtttttgt
    35041 ttaaagggac atgctgattt tgctgaaata ggctatgata tagtatgtgc tgcagttaca
    35101 tctaatgcta ttgcagttat aaattcttta gacaaattac agaagattga atttgaaaaa
    35161 gtagtaggag aagaaggtca tatagagtgt atagtcaaag atacacatgt aaaagacgca
    35221 caattattac taaatcactt tcaattagcg gttaaagaaa ttaaacggga atacccaaaa
    35281 aatataaaaa ttttaaaaaa gtagggggtg actccatatg ttaaacatga atttacaatt
    35341 attagcatct aaaaaagggg taggttcttc taaaaatggt agagattcta tatctaaaag
    35401 attaggtgtt aagagattcg atggtcaatt agtaactgct ggaagtataa tagttagaca
    35461 aagaggaact aaaatacatc caggaactaa tgttggaaaa ggtagtgatg atactttatt
    35521 tgcattagtt gatggtactg ttaagttcga aagaaaagac aagaagagaa aaaaagttag
    35581 tatatatcca gtagcaatag ctgagtaata tacataaaag gagtgttgag gcactccttt
    35641 ttcttataat gctgatgaat ttataatggt atttttggta atgctaatta aaagttttta
    35701 attaagtagg tgaagaattt gtttatagat aaggcaagaa tatttgttaa ggctggaaat
    35761 ggtggaaatg gttcggtagc atttagaaga gaaaaatacg ttccagctgg tggtccagac
    35821 ggtggagatg gtggtcgtgg agcaagcatc atatttgaag ttgatttggg gcttagaact
    35881 ctaatggact ttaagtacca gaaaaaatat caagctcaaa atggtggcga cggaagcaaa
    35941 ggaaaaagag ctggtaaaaa tggagaaaat ttagtattaa aagtaccagc aggtacagta
    36001 ataagagatg aggctacagg tcttgtatta gcagatttaa aaaaagaagg agatacagct
    36061 atagttgcaa aaggtggtat aggtggtaaa ggtaaccaac actttgcaaa tgcagtaaga
    36121 caagctccag cttttgcaaa atctggtaca gatggagaag aaagatggat aactcttgaa
    36181 cttaaaatga tagcagatgt tggtctttta ggatttccaa atgtaggtaa atcaacattt
    36241 ctatcagttg taactaaagc taaaccaaaa attgcaaact atcattttac aacacttaca
    36301 ccaaatctag gtgttgttca aactaagttt ggagatagtt ttgtacttgc agatatacct
    36361 ggaattatag agggtgcttc tgaaggcata ggattaggtc atgagttctt aagacatgtt
    36421 gagagaacaa aggtattgat acatattgta gatatatctg gattagaggg aagagaccct
    36481 attgaagatt ttgacaagat aaatgatgag cttaaattgt acaatgaaaa gctttcaaag
    36541 agaccacaag ttgtagttgc aaataaattt gatatattag aagatgagag taagtttgag
    36601 aagtttaaga gtgaattaga aggcagagga tatacagtat ttaaaatgtc agcagcaact
    36661 cgtcaaggca tagatgaagt aatagcctat gtttctaaaa tgctaaaaga agtagaagat
    36721 gtggagctag tttcagaaga agaaatgtat agaccagaac ttgatattgg tactgaagaa
    36781 gagttaagta tagatattga agatggtgtg tatgtagtta caggtaaagc acttagaaga
    36841 ataatgtatt ctgtaaattt tgatgatatg gaatcccttc aatatttcca aaaagctatg
    36901 gagagtcaag gtgtttttga taggcttaga gaaatgggaa tagaagatgg agatgtagtt
    36961 aaaatttatg aattagaatt tgagttttac aattaattta aatatagatt atagataatt
    37021 taagtataga attatagagg tgagaatatg ttaaaaggaa aacaaagggc gtatttaaga
    37081 tctttggcaa atactttaaa gcctactaca caaattggta aagaaggtgt tacagagagt
    37141 ttcttagagc aattagatgg tatgttaaga actagagaat tagtaaaagt tactatatta
    37201 gaaaatgcag gtcttgatac taaagaaact gctaatgcag tatgtgaagc tttaagagct
    37261 gaatttgtgc aggctattgg ttttaaattt actatttaca agaggaatat tgaagagcct
    37321 aaaatacttt ttccaggaca tgaacaggcg aaagcaaaga ctaaaaataa taatgttaca
    37381 aaaaagggta aaccaactaa aagagctgtt agataatagt ttatatcctt ttagggaaat
    37441 aagttaaaaa aatagtgaca attggaagaa attgtattat actattaaac ataaatttaa
    37501 attagcaatc ttatcaagag tggcagaggg actggccctg tgaagcccgg caaccagtat
    37561 atttaattat acattggtgc taaatcctgc agcatttatg ctgaaagatg agtataaacc
    37621 cgtcatatgg cttgagttta tactcaagct tttttaattg gtatatttat acatataaat
    37681 aatttaatat aatcaaaatg atgggggtat gtaacagtga agatagcaat attagggtat
    37741 ggaacagtag gaagtggatt aattgattta atagaaaata atacttcaaa aagagatata
    37801 gaagttgttg gaattttagt taaaaataaa gaaaagcaca agtataagaa gtactttgat
    37861 aagataacta ctgatataga agatatattt gacaaggata ttgatatact agttgaagta
    37921 attggaggat taaatccagc ttttgattat gtaacaagag cgttaaataa aaaaatacat
    37981 gtagtgacag ctaataaaga tttactggct gaaaaaggta gtgaccttat agaattagct
    38041 aacttaaata atgttagcat caagtttgaa gcatctgtgg caggtggtat tccagtttta
    38101 aaaccattga tagaatcact ggaaggcaac aatataaaga gcataaacgc tattttaaat
    38161 gggacttgta attttatatt atcaaagatg tatgatgaaa atttaccgta tgatgtcgct
    38221 ttaaaacagg cacaagagtt gggatttgca gaagttaatc cagatgcaga tgttttagga
    38281 tatgatagtg ctagaaagtt atctatattg tctactttat cttatggaaa aatagtatat
    38341 tggaaagatt tattattaga aggaataact tcaatagatg aaaaagatat agaatatgca
    38401 aaaaaattaa attgtaagat aaaattggtt gctagaagta agtatgaatc aggagaagta
    38461 agtggatttg ttagaccagc attagtggac aataataata tgctttcaaa gatagataat
    38521 gagttcaacg tagttatttt agtaggtgat tcagtaggtg aactttcttt tgtaggtaag
    38581 ggagctggaa gaggtgcaac aggaagtgct gtttatgcag atattataga tataatagat
    38641 aatagaagtt caaatataaa gtctttttca aaaggtaaat tggatttaag tggacttata
    38701 gaagatgagt gtagtgcagt agttagattt ggtgggtata ataatgaaga tggaatttta
    38761 gaatgtttag ataaatatgt agatagttat gatattatag atgatgaaga attggcgatt
    38821 tttgtaagtg caaagtcaga acatgagatt gatagagttt tggaaattgt aaaaagaaat
    38881 aattacagtg aatcagctaa aaaattattg aagatagatt aatatgttta tgcaagaatt
    38941 atgaaaattt aatagggtgt ctcaaaatag agagataagc cttttttcca gtgttgaaat
    39001 aataaaaata tatttgattt tataaaaaaa gagtgtctca tgaactaaaa agttcatttt
    39061 aagacactct tttttaatat ctggtgaatt aataagataa tagaaaaatg tatacgttat
    39121 aattaaccaa taaagaaatt ttcactagct tcattaatag cttgctgagt tacccttata
    39181 tatcgctgtg tagtggctac agagctgtga tttaaaagtt ctttaaccaa ctctaaatta
    39241 ttgttactct tttcaaattg agtagtagca tagagctttc taaagctatg tgtacttata
    39301 ttatacagat ttaagtgttt acaaattatc cttaactgtt tttgaatagc ctttgtcttt
    39361 atatttacta agtagtcatt tgatttaaga ttatactttc ttgcatgttc atttatggac
    39421 tcaattatat ttttattaac tggtctatac tgtaatttgc ccgtcttttt ttcatttatt
    39481 tccaacatat tacctttaaa attacctatc tttaatctta aaatatctga tattcttaga
    39541 cctaaattag cttctaacat tagtgccaat gcaactttag gattttttct aaatcttttt
    39601 tcaacgccat tttcactata agtaaagcca gtatgtaata gttccattat ctttttatat
    39661 tcgtctattt ctataggtct tgttgccatt ttcaaccact cctcttagtt tgatttggta
    39721 agtttaggtc ggtatttatt atataatata aatgaaatat tatataatgg aaaaatttcc
    39781 cag
//
LOCUS       DAEMHW010000013        39342 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEMHW010000013 DAEMHW010000000
VERSION     DAEMHW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39342
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.013"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..380
                     /locus_tag="KPK67_000576"
     CDS             <1..380
                     /locus_tag="KPK67_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG2457583.1"
                     /translation="RQFSGGYHADNYFRCLLTFIFIILSTILIIANINIDLFKNIIMI
                     IASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSLSLSIFVDYF
                     TYSAFAMFWIFVMLVLGKLKAKV"
     gene            410..556
                     /locus_tag="KPK67_000577"
     CDS             410..556
                     /locus_tag="KPK67_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG2457584.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            861..2240
                     /gene="scfB"
                     /locus_tag="KPK67_000578"
     CDS             861..2240
                     /gene="scfB"
                     /locus_tag="KPK67_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBG2457585.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPSGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            2269..2766
                     /locus_tag="KPK67_000579"
     CDS             2269..2766
                     /locus_tag="KPK67_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBG2457586.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            3064..4728
                     /locus_tag="KPK67_000580"
     CDS             3064..4728
                     /locus_tag="KPK67_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBG2457587.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            4741..7191
                     /gene="recJ"
                     /locus_tag="KPK67_000581"
     CDS             4741..7191
                     /gene="recJ"
                     /locus_tag="KPK67_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG2457588.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            7204..7716
                     /locus_tag="KPK67_000582"
     CDS             7204..7716
                     /locus_tag="KPK67_000582"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBG2457589.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            7790..9997
                     /locus_tag="KPK67_000583"
     CDS             7790..9997
                     /locus_tag="KPK67_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBG2457590.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            10022..10471
                     /locus_tag="KPK67_000584"
     CDS             10022..10471
                     /locus_tag="KPK67_000584"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBG2457591.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            10486..11100
                     /locus_tag="KPK67_000585"
     CDS             10486..11100
                     /locus_tag="KPK67_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2457592.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            11191..12747
                     /locus_tag="KPK67_000586"
     CDS             11191..12747
                     /locus_tag="KPK67_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBG2457593.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIKNMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            12737..13999
                     /locus_tag="KPK67_000587"
     CDS             12737..13999
                     /locus_tag="KPK67_000587"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBG2457594.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIAIAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            14073..15860
                     /gene="aspS"
                     /locus_tag="KPK67_000588"
     CDS             14073..15860
                     /gene="aspS"
                     /locus_tag="KPK67_000588"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBG2457595.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLKHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            16319..17551
                     /locus_tag="KPK67_000589"
     CDS             16319..17551
                     /locus_tag="KPK67_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG2457596.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            17565..18371
                     /locus_tag="KPK67_000590"
     CDS             17565..18371
                     /locus_tag="KPK67_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBG2457597.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEINLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(18483..19352)
                     /locus_tag="KPK67_000591"
     CDS             complement(18483..19352)
                     /locus_tag="KPK67_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2457598.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            19724..21151
                     /locus_tag="KPK67_000592"
     CDS             19724..21151
                     /locus_tag="KPK67_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBG2457599.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSISNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(21226..21495)
                     /locus_tag="KPK67_000593"
     CDS             complement(21226..21495)
                     /locus_tag="KPK67_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457600.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(21498..22940)
                     /gene="nhaC"
                     /locus_tag="KPK67_000594"
     CDS             complement(21498..22940)
                     /gene="nhaC"
                     /locus_tag="KPK67_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG2457601.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(22965..24131)
                     /locus_tag="KPK67_000595"
     CDS             complement(22965..24131)
                     /locus_tag="KPK67_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2457602.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(24356..26476)
                     /locus_tag="KPK67_000596"
     CDS             complement(24356..26476)
                     /locus_tag="KPK67_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2457603.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRFNKLNS"
     gene            26704..27105
                     /locus_tag="KPK67_000597"
     CDS             26704..27105
                     /locus_tag="KPK67_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457604.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            27148..27441
                     /locus_tag="KPK67_000598"
     CDS             27148..27441
                     /locus_tag="KPK67_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBG2457605.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            27456..27896
                     /locus_tag="KPK67_000599"
     CDS             27456..27896
                     /locus_tag="KPK67_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBG2457606.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(27988..28461)
                     /locus_tag="KPK67_000600"
     CDS             complement(27988..28461)
                     /locus_tag="KPK67_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG2457607.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(28461..29249)
                     /locus_tag="KPK67_000601"
     CDS             complement(28461..29249)
                     /locus_tag="KPK67_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG2457608.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            29577..30821
                     /locus_tag="KPK67_000602"
     CDS             29577..30821
                     /locus_tag="KPK67_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG2457609.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            31144..32241
                     /locus_tag="KPK67_000603"
     CDS             31144..32241
                     /locus_tag="KPK67_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG2457610.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIRDLIYDDATLKKMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            32232..32930
                     /locus_tag="KPK67_000604"
     CDS             32232..32930
                     /locus_tag="KPK67_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2457611.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            32933..33997
                     /locus_tag="KPK67_000605"
     CDS             32933..33997
                     /locus_tag="KPK67_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG2457612.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            34180..34884
                     /locus_tag="KPK67_000606"
     CDS             34180..34884
                     /locus_tag="KPK67_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2457613.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            34877..36199
                     /locus_tag="KPK67_000607"
     CDS             34877..36199
                     /locus_tag="KPK67_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2457614.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            36386..37759
                     /locus_tag="KPK67_000608"
     CDS             36386..37759
                     /locus_tag="KPK67_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457615.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(38131..39069)
                     /gene="pdaA"
                     /locus_tag="KPK67_000609"
     CDS             complement(38131..39069)
                     /gene="pdaA"
                     /locus_tag="KPK67_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG2457616.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT        15043 a         4535 c         6696 g        13068 t
ORIGIN      
        1 taagacaatt ttcaggaggg tatcatgcag acaattattt tagatgctta ttaaccttta
       61 tttttataat tttatctaca atcttaatta tagcaaatat aaatatagac ttatttaaaa
      121 atattattat gattattgca agtgttagtt gggttggaat ttgtgttctg tgcccaatag
      181 aacataggag taaccctata agtgataggg aaaagttggt ttataagaaa actgctatat
      241 ttatatctac agtagtgtta ttgataacaa ttttaagtct ttctttgagt atatttgtag
      301 attatttcac atatagcgca tttgccatgt tttggatttt tgtgatgtta gtattaggta
      361 agttaaaagc taaagtataa gtaaaatatg tatgagggag agttaagtta tgaaaaagtt
      421 tattgtaaga tttatgaagt ttgctagctc attggcactt agcactgcta ttttgtcagc
      481 taatagtacc tgtccttgga ttattcatca gcctaaagta cccaaagaaa tcagcaatct
      541 aaagaagaca aattaaatac aagcacttat ttatacaaaa ttatcaggaa aaaagaaatt
      601 attatatttt aataagatta aaacaatata aaatcagata caaaatttat tttatttatt
      661 ttataggagc ctaataccta tttggtaata tattagtgcg ccaatttagg gggatatagt
      721 gtatttaatg aaatgactta cggagagtaa gtcatttttg tattgtatgt attttatgta
      781 aacttatgat ataatgaagg gtaaagttta attaaaataa taaaaaactt tagtatagat
      841 agtaatttag gaggtagggt atgagtatga tacataaatt ttctatgaat ggatataata
      901 tcgttttaga tgtgaatggt ggagctgttc atgtgttaga tgatgttgca tacgatttgt
      961 tagattttta taaagaaaaa agtaaagaag aaatattaga gatattaaaa tcaaaatatc
     1021 aagaagaaaa aataaatgaa gcatatgaag aaatattaaa tttggaaaaa gagggtcttt
     1081 tatatacaga agatacatat caatatcatc caagctttgt acatagagag cctgtagtaa
     1141 aagctttatg tttaaatgta gctcatgatt gtaacttaaa atgtaaatac tgttttgcag
     1201 ctcaaggtga ttttggagga gaaaaagaac ttatgagttt tgaagttggt aaggcagcaa
     1261 ttgactattt gattgctaat tcaggaagta gaaagaacct agagatagat ttctttggtg
     1321 gagagccttt gatgaacttt gaagttgtaa aacaactggt tgactatgga agaagtgtag
     1381 aaaaagatta taataaaaat ataagattta caataactac aaatggtgta ctgctaaatg
     1441 atgagattat agattatata aatgaaaata tgcataatgt agttttaagt ttagatggaa
     1501 gaaaagaagt taatgataat atgagaccaa ctttaaatga taaaggaagt tatgatatta
     1561 cattaccacg atttaagaaa cttgtggaaa aaagagcaaa agataagtat tattatataa
     1621 gaggtacttt tacaagagat aatttagatt tttcaaaaga tgttatgcat tttgcagatt
     1681 taggatttaa attaacctct gtagagccag tcgttggaga tgagagcaat ccatatgcac
     1741 ttagagaaga agatttacct aagatatttg aagagtacga aaaatttgct gtagagtatg
     1801 cagatagaca attacaagga gatggattta aatttttcca ttttatgata gacctaaatc
     1861 aaggtccatg tgtaataaag agaataactg gatgtggagc tggaaatgag tatctttctg
     1921 taactccaag tggagatatc tatccatgtc atcaatttgt tggaaatgaa gaatttaaaa
     1981 tggcaaatat atttgatgaa gaaatagtac ttccagaaaa tttaaaaaat atgtttagag
     2041 aagctcatgt atatactaaa gaagagtgta aacaatgttg gaataagttt tactgttcag
     2101 gtggatgtca tgcaaatgcg ataaacttta ataatgacat atcaaaacca tatgaattag
     2161 gctgtgaaat gcaaagaaaa cgtacagagt gctctataat gatacaagct aagttaatgt
     2221 tagaaggagc aaccaactaa atttatattt taaaggagtt gtttatatat gattagagat
     2281 tatttagaag ataaaccttt aattgacgaa agtgtatttg tggcaaaatc agcagatgta
     2341 attggaaatg ttaaaatagg caaagattca agtatatggt acaatgcagt agttaggggt
     2401 gatgaaggac caataactat tggagaaaac actaatatac aagattgttc tatagtgcat
     2461 ggagatacag aaactataat tggtaacaat gtaacagttg ggcataggtc tatagttcat
     2521 ggttgtaaaa ttagtgataa tgtgttaatt ggtatgggtt caataatatt ggataatgct
     2581 gaaataggag aatatactct gataggagca ggtacactaa ttacatctaa taagaagttt
     2641 ccaccaggtg ttttgataat gggttctcct ggtaaggttg ttagagagct tacagaagaa
     2701 gataaaaaat atattgatga atcttatgaa tggtatttag aggcagcaca aaatcaaaaa
     2761 tattagttta aagagtggtt gtttcaaaac atatttaaat ctgttttgag gcaaccacta
     2821 atttatttta ttagattttg ttgatatttt agttttcaaa aagtatttga ttaaaccaga
     2881 tataaaatgt caaaaataaa aattaactga gttaaataat tttaataaaa ttggtagtat
     2941 atgtgataaa attaaatatt aaagaggata taggatatta gaatatcaat tggtgaaata
     3001 atgtcatata cgataaatta aggtgtaagt ttttgggtat acctatctta ggagtgaaaa
     3061 gatttgagaa taagttatag aaatttaaag cgatatagag aaattggata tgttttaata
     3121 aagtatggtt ttagttttat tgtagaaaga ctaaatatag aaggaattgc ctataaaata
     3181 ccactatttg acccaccaga agaaataaaa aatatgacaa ctggtgagag aatgaaaaga
     3241 gttttagaag aactaggacc tacatatata aaaataggac agatattgag cacaagaaaa
     3301 gacttgttag accaggatat aattgatgaa atatcaaaac tcagagatga tgtagagaaa
     3361 tttgattcaa atatagctat tgatatattt aaagaagaag tgggtttaag tatagaagaa
     3421 atattcttag aatttaaaga agaaccaata gcagcagctt caattggtca agtttatgaa
     3481 ggtgtactta aaacgggaga agaagtaatc gtaaaaattc aaagaccaaa tatagagaaa
     3541 ataataaaat ctgatttaga gattcttaga actattgcaa atactttaaa agatttaaaa
     3601 aaggatttta atttagattt agtacagatg attgaagaat ttcaaactca attaatgagg
     3661 gaacttgatt atacatttga agctataaat gcaactaaat tctctaggat atttaaaaat
     3721 agtgatgaag tatatatacc taaagtatac tcagaatata atactaagaa aattttagta
     3781 atggagaaag ttaatggtac aaagttaagt gatgtagaaa aaataaggag actaggttat
     3841 aacacaaaaa ctattgttga aataggcgtt aggtcatttt ttacacaggt attatctcat
     3901 ggattttttc atgcagaccc acatccaggt aatatattcg tagttgctaa aaataagata
     3961 gcatatatag attttggaat gattggaatt attgataata aaactttaaa ccaattaaat
     4021 gaaattgcat tagcaggtgt agaaaagaat gttgacaaga taatatatct attgatagaa
     4081 atggatgcat taaatggaga ggctgatatt aagggactta gacaggattt actttatcta
     4141 atacattatt actatgatat ttctatagaa aaaataaatg taacagatat attaaatgaa
     4201 ctatttaggt tttttagaca gtataaaata gtcatgcctg cacaatttgt aactcttgca
     4261 aagacagtta taacattaga aggaacaagt agaactttaa acacagattt ttcttttggt
     4321 tctatgggaa aagagtttat gaaacatcat tataagagta aatttaaccc taaaaatgtt
     4381 gttttaagtt caagacaaaa tgtagaagaa atattactgg acataaaaac tattccaaag
     4441 caattaaaag caattttaaa aaatatagaa agaaataata taaaaatgca aattgaagat
     4501 gttaaaatga cgaggttaga aaattgtata atagagctaa catctcaaat atctttgagt
     4561 ttagtgttgg catctattat agtaggttct tctcttataa ttgcatctcc aaatatagaa
     4621 aataatatat ggataaaatt tactgcaata gcgggtttct ttatatcctt tataataggc
     4681 ctttgcttag ttataagaag tattaggtca aaatacaaaa aagattaggg ggaattaaat
     4741 ttgaaaaaat ggatattaaa gtataaaggt aagatagagg aaagtgaatt tagcaaaaaa
     4801 cttaatattt ccccggaaat ctgccaaatt ttaaaaaata gagggatatg tactgaaaaa
     4861 gattcagaaa tatttatgaa tccatcttta gattatttaa gagacccttt tttaatgaaa
     4921 gatatgcaaa aagctgttga tagaataaag ttagcagttg agaagaacga aaaaatttgg
     4981 atttacggag attatgatgt tgatggtgta tcatctacat ctatcttgtg cttatatttt
     5041 aagagtatag gttacaatgt tgattactat attcctaata gattagaaga agggtatggt
     5101 ataaatgaag atgctattaa gcttataagc tctaggggat gtgacttgat tataagtgtt
     5161 gactgtggaa ttacatcagt gtctgaggtc aatacagcga atgatttggg catagatgtt
     5221 ataattacag accatcatga atgtcaaagt gaaattccat cagcctttgc agtaataaat
     5281 ccaaaacaag aagattgtaa ttatccattt gattcactat gtggatgtgg tgtagcattt
     5341 aaaatgattc aagccttgac tccaaaagaa gaatttaaaa ctagtatgta taattattta
     5401 gaaatagtaa cactagcaac tatttgtgat attgttccac ttatagatga aaatagaata
     5461 attgtaaaaa atggtttaaa atcaatgaaa gaaggcaaaa atattggatt aagggagctt
     5521 ataaaagttt gtggtgtaga aagtgataag ataggttctt ctcatatagg ttttgcaata
     5581 ggtcctagga taaatgcttc aggtcgattg ggatattctt acttaggagt agagctattt
     5641 actactcaat cacaagaaga ggcagtagaa atagctagta tattggaaga aaaaaataat
     5701 gaaagacaga tgatagaagc taagatgtat catgaagctg aagaaatgct aaagtctaat
     5761 agcagataca atgatgacaa ggtgttggtt ttagctaaag agggttggca acatggaata
     5821 ataggaattg tagcatcaaa gttaactgaa aaatactaca aaccaacaat tcttctaggt
     5881 atagaaaatg gggaggctac tggttcagcg aggtcaataa aaggatttaa tatatttgaa
     5941 gctctcataa agtgcaaaga cttgatgact aagtttggtg gtcatgaaca agcagcaggg
     6001 ttatccttgg attcagacaa tgtagaaatt ttagcaaatg aaataaataa atttgcagat
     6061 tataatttaa ctgaagatga tatgatagag aatgtaaatg ttgagtttga attacaagaa
     6121 aatgtaataa atttaaatct tgtagaagaa ttacataagt tagaaccctt tggattaaat
     6181 aatcctaatc ctagatttat agttagaaat tatattttaa aagatttgaa agttataggt
     6241 aagaatcaac aacatctgaa attaagtata gaaaaagaga aaagctatga atgtatagga
     6301 tttaatatga gtcatttaaa atcaatgtat aaagtaggag acaaagtaga tgtactattc
     6361 caattagatg aaaataacta tatgggaaat aggaaagtac aatttttact taaagatata
     6421 cgattggctc gacctaaaag tgcaagtaat gacaaacttt cattgaaatt aatgtcaaaa
     6481 ataataccaa aagatactca aagcctgtat aatatatctg tgtctgattt tgaattattt
     6541 gatggaaata cagatataaa tatatttgat tattttgaaa aagatacact aataatatct
     6601 aattctataa atggatttta tagagcaatg tcagatatat cacttataga tttggatttt
     6661 aacataaatt acaatattat tgaagatgtt agcaaaaata ctgataaatt agagcttata
     6721 ttttctccta atattgataa aatagactta aaaagatata ataatattat tctttatgat
     6781 tatttataca ataaaggcga atattcgtat atatatgaga ataaaagaga agaaagtgaa
     6841 attataaaat attataataa aacagaccta ttatatttaa agaatgtagt tagtaatata
     6901 gttccatcaa gagatgaatt tataacaatt tataaacaag cactcataaa aaaagaaatt
     6961 gatttggata tggtaaatat aagggaaact tttaatgtaa ttccattaaa gttttttacg
     7021 atattaaatg tatttagaga attaaattta cttgacttta atttaaatta tgaaaaaaat
     7081 tctgtactca taagaatatt gccaaaacca cagaaaaaat tagacttaaa tgagagttta
     7141 atactcaata atctaaaaaa tttagaaaaa caatacaata gcagttatta ggaggcgttt
     7201 cccatggatt taaaaaactt tataaggaat attgatgatt tcccaaagcc aggcatagat
     7261 tttaaagatg tgacaactct atttaaagat ggagatgcat ttaaatatgc agtggattct
     7321 atagttgaag aattgaaaga taaagatgta gatttagtaa tagggccaga agcaagagga
     7381 tttttgatgg gtacacctgt agcatatgct ttaggagtag gatttgttcc aattagaaaa
     7441 cctggaaagt taccaggtga agttgagagt tatgagtatg gattagagta tggtacagat
     7501 actttagaga tacataagga tgcaattaaa aagggtcaaa aagtagcaat agtagatgat
     7561 ttattggcta ctggtggaac tatggaagcc gctgccaagc ttgtagaaaa attaggtgga
     7621 gaagtagttt ctatgcaatt tttgattgag ctaaaattct taaatgggag agaaaagttg
     7681 tctaactatg atgttaattc tcttataaaa tactagtact tttataatag ttggtataac
     7741 accaactatt ttcatatata ttaaaatttt atacaaaata ggtggttata tgcatgataa
     7801 agaattacaa gaaataattg aaaaaataaa agtttatgca cctaatgcaa attttgaaat
     7861 ggttgaaaaa gcctataatc ttgcaaaaaa agcgcatgaa ggacaatata gaaaatctgg
     7921 ggaaccatac tttattcatc cattagcagt tgcaaatatt ttaatagaga tggagttaga
     7981 tattgaaact ataacagcag gtttacttca tgatgttgta gaagatacag aatataccta
     8041 tgaagacata aaaaaagaat ttggcaaaga agtagcggat ttggttgatg gagtaactaa
     8101 acttggtcaa ataaaatatc gttcgaaaga agaaactcaa tctgaaaatt taagaaaaat
     8161 gtttttggca atggcgaagg atataagggt tatacttata aaacttgctg atagattgca
     8221 caacatgaga acattaaaat acatgcctcc agaaaaagca aagtataaag caaaagagac
     8281 acttgaaatt tatggtggta tagctcatag attaggaatt tccaagataa agtgggaact
     8341 agaagacaga gcattaagat ttatggaccc agaagggtat tatgatttag ttagtagagt
     8401 atcaatgaag agaagtcaaa gagaggatta tattcaagga attgttgaac ttcttaaaga
     8461 taagtttaaa gatttaagta taccatgtga tgtatatggt agaccaaagc atttttacag
     8521 tatatataga aagatgcaaa aaaaacataa gacttttgaa gagatttttg atttaacagc
     8581 agtaaggata ttggttgata atgtaaagga ttgctatgca gtacttggta tagttcatac
     8641 attatggaga cctattccag gaagatttaa agattatata gcaatgccaa aacccaatat
     8701 gtatcaatca ttacacacca ctgttgtagg tcctgatgga gaacctttag agatacaaat
     8761 aagaactcat gagatgcata atatagctga aaatggtata gcagcccatt ggaaatataa
     8821 agaaggtatc tctagcaaag agtcaaaggt tgaagaaaaa cttcaatggt tgagacagat
     8881 gatggagtgg gaaaaagatt taaaagaccc acaagagttt atggatgcac ttaaagaaga
     8941 tgtatttaat agtcaagttt atgtatttac gccaaaagga gacgtaattg aattaccagc
     9001 agaatcaaca cctatagatt ttgcctatag ggttcataca aatgtaggta ataaatgtgt
     9061 aggagctaaa attgatggaa gaatagttcc tattgattat aagcttcaaa atggtaatat
     9121 tgtcgaggta cttacatcat ctaattctaa tggaccaagt agggattggc ttaatatagt
     9181 gaaaactcca aatgcaaaaa gtagaattag acaatttttc aagaaggaaa gaagagaaga
     9241 aaatgttgac cgtggaaata caatattaga aagagaattt aagaagcatg atataccaca
     9301 aaaagaccct cttgttgaaa aatatatggc attagttgct aagaaattta atcagccaag
     9361 tgttgaggac ttaattgcca ctgtaggtta tggtggtata atgtcatctc aagtggtatc
     9421 taaggtaaaa gacctatatt taaaagaggt taaaaaagct aaaaaggaac aacagataca
     9481 agaagaagat ttaaataaac acaatataag tgaaacagaa tacagtaaaa agagaaagaa
     9541 aaacgcatca caaggaatta tagtaaaagg gcttgacaat atcttagtaa gatttgctaa
     9601 gtgttgtaat cctcttccag gagatgaaat tgtaggatat attactaagg gtaggggagt
     9661 agccatacac agagctgatt gcccaaatgc aaacaataat ggtgatttct tccaaaatag
     9721 attggtggag gtttcatgga gttcagcagg aaaaggtaag tttgaagcgg aaattcaaat
     9781 aaaagctatt gacagaagag gaattgttaa tgaaataact catattgttg caatggataa
     9841 acttagttta aacggaataa atgcaagaaa aggtaaggat agtattgtaa gtataaactt
     9901 acttgtagag gttaatgaca cagaggaatt aaaaattcta atgaaaaaat taaaagcaat
     9961 tcctggtgta gaaagtattt atagaatgac aaattagagt taaaataata gggaggaaac
    10021 tatgagagca gtagtgcaaa gagtatcttc atctaaagtt acagtagatg aaaacactat
    10081 aggtcaaata aataaggggt tactagtatt gttaggagtg actcatgatg ataaatcaag
    10141 tgatgtagat tatatgattg ataaaatatt gaatctgaga atttttgaag atgaaaatga
    10201 caagatgaat ttatctctaa tggatatagg tggagagctt ttagtggtat ctcaatttac
    10261 tttatatgga gattgcagaa aaggaagaag acctggattt agtaatgcag ctagaccaga
    10321 acttgcaaat aacctttatg aagaatttgt aaaaaaagca aaagataaag gtgtgacagt
    10381 tggtacaggt caatttgcag ctcatatgat ggtagagtta actaatgatg gtccagtaac
    10441 aatactatta gactcaagta aatcttttta agggggaaaa ctttaatgat tataaaaaaa
    10501 tttgtagata gttattttgg agtaaatact tatgtattag gtgatgaaaa gactaaaaaa
    10561 tgtgcagtta tagacccagg tggaagttta gtagagacat taagttttgt aaaagaaaat
    10621 gaattaaata ttgaatatat tatccttact catgggcatg gagaccatat aggatatgtt
    10681 aaggatatta aagaaaaaac tggtgctaaa gtagttgcac atgttgatga aaaagaatta
    10741 ttaaatgata aaaataaaaa tctaagttat acaatgagat gtggagcgca agagtttgat
    10801 gcagatatct atgtgaatga taaagacaag cttgaacttg gtgaattaaa aatgactttc
    10861 ttgcatactc caggacatac tcaaggatgt atgtgtataa gagttgaaga tgaaatgttc
    10921 acaggagata ctttgtttgc tggaagtatt ggtagaactg atttatatag tggggatttt
    10981 aaccaaatgg aaaaatcact taagaaatta tgcaaatatg aagatgctgt tagagtatat
    11041 cctggacatg gaccaaccag tactctaggg gttgaaaaga agacaaatcc ttacatgtaa
    11101 ttttatatta gtatttaaaa aagtataaaa tatttaataa ctatattagt gagggggttc
    11161 ttctactgtt aaatagaagg agttaagttg atgttgtatg tttttttaaa aggacatgat
    11221 tataagtatg aagtagcaga acttataaaa ttgtttacca gtgaatttaa gtttaaagac
    11281 tctaatgcta acaataatat agagtcaaag catttaataa atagattgat ttatgagaat
    11341 ggtgtattat tctcaagtac tgaatactat gaaaatggaa acttaaagta tgagtcaata
    11401 cagaatataa aaaatatgaa tcttgaattt ttagatggat ttgatagtag caaaacgatt
    11461 gaagaatttc ttaatagcct agatagtgaa agtaagagga actttaaaaa gctgtgtaaa
    11521 gaaaatatca aaaaaagcat gtttttagtg cttaaacaag tatttaattc ttatgtacca
    11581 tggggaattt tgactggtat tagaccagtc aaaattgtcc acaatttaat ggataaaaag
    11641 ctagatgata atagcataag aaccattcta aaagataatt attttataat agatgagaaa
    11701 atagatttag ctcttgaaat agctaaaaga gagcgattat ttatgtatcc tatagataaa
    11761 aataaaattt ctctttatgt aagtattcca ttttgtccaa ctagatgtgt atattgttca
    11821 tttccttcaa attcattaaa acaatttgga catttaaaaa gagaatatgt ttataaattg
    11881 attgaagaaa ttaaggggtt tgccaaagtt attaaggata ctaagaaaga agttgaaact
    11941 ctatatattg gtggtggaac tccaactaca cttgatgagg aagaattgga tttgcttata
    12001 aattcattgt tcaatgaatt agatttaagt aaaattaaag aatttactgt agaagcaggt
    12061 agacctgaca ctattacaga acaaaaacta agagtgttaa aaaaacacaa tgttagtaga
    12121 attagtataa atcctcaaac tatgaatgat gatactttag taaagattgg aagagagcat
    12181 agagtatctg atttagtaga ttgctttaat atggctcgta gaatgggatt tgataatata
    12241 aatatggata taattttagg tcttatagat gaagatttaa atatggtaaa gaatacattg
    12301 gaggagataa aaaaactttc tcctgaaagc cttacagttc atacattagc cataaagaga
    12361 gcatccaact taaatataaa tatggatagg tataaagaac atctaacaca atatgaagaa
    12421 atggtaaaaa tgatagattt atcaatgaaa tatgcaaaag atatgggatt aaatccatat
    12481 tatatgtaca gacaaaaaca tatgttggga aatttagaaa atataggtta tgctaaagaa
    12541 ggatatgagt gcatttataa tatacagatt atggaagaaa aacaaagtaa ctatgcatta
    12601 ggtgcaggtg ctatatcgaa atttgtttat gtagatgaag atagaattga aagagtagaa
    12661 aatgtaaaaa atgttgaaca atacattgaa agagtagatg aaatgataaa aaggaagaaa
    12721 gaagaggtat ataaaaatgt tgactaaagc tcctagagga actaaagaca taactccaaa
    12781 agaggcttac aaatggcgtt atgtagaaaa taaatttaga gaaatatgtg ctttatatgg
    12841 atatgaagaa atggtaacac ctatatttga acatacagaa cttttcaaga gaagtgttgg
    12901 agatactaca gatatagttc aaaaagaaat gtattctttt aaagataaag gtgatagaga
    12961 aattacccta aagccagaag gtacagcagg tgtagtaaga gcatttatag aaaataaatt
    13021 atatgcagat actcaaccaa caaaattatt ttatgtgaca ccttgtttta gatatgaaag
    13081 accacaagct ggtagacaaa gacaatttca ccaatttgga atagaagcac ttggaagtga
    13141 tactccttca atggatgctg aaataatcgc aatagctgtt caatttttta atgaagttgg
    13201 attaaatgat ttggtagtga gtataaattc agttggctgt ccagtttgta gaaaagagta
    13261 caatgcttta ttaaaagagt atcttgactc aaaagctgat atattgtgtg atacttgcaa
    13321 tgagagaaga gaaaaaaatc caatgagggt tatagactgt aaaaatccta catgtaaaga
    13381 aaatatcaaa gatataccat ttattgcaga ccatttgtgt gatgattgta aatctcactt
    13441 tgataaactt caagaatact taaaggaaat gaatataaac tttgtaattg ataaaacaat
    13501 agttagaggc ttagattact atagaaaaac tgcttttgaa attatatcta atgatatagg
    13561 agctcaaagt actgtatgtg gtggaggaag atatgatggt cttgttgaac aattaggagg
    13621 accaaaaggt ataagtggaa ttgggtttgg actaggaatt gaaagacttt tacttacact
    13681 tgaaggtaat ggaattgaaa tagaaaaccc tcaatctacg gatatattta tagtaactat
    13741 aggtgaagaa gcaaatacaa gaagctttaa attgttaaaa gacttgagac aaaatcatat
    13801 aagtgcagat aaagatcata tagagagaag tgttaaagca caatttaaat attcagataa
    13861 aataaattca aaatttacaa tagtaattgg agatgatgaa cttaaaaatg acactgcgac
    13921 attaaaaaat atgaaaacat cagagcaaac tactgtaaaa ttaagtacat tagttgaaga
    13981 attaaagcaa aagctgtaaa ataagaaagt atgatgtttt gtgtattaga taaatatata
    14041 gcattaaaat taaataaaat ggaggtagta tcttggaaac tttaaagggt ttaaagagaa
    14101 ctcactactg tggagagttg agagaaaaaa acataaatga agaagttgta cttatgggat
    14161 gggtacagaa aaaaagaaat ttaggtggtc ttgtatttgt tgatttaaga gatacaagtg
    14221 gattatgtca aatagttttt gatacagatg tagataaaga agcttttgaa aaagctgaaa
    14281 aattaggggc tgaatttgta atagctgtaa aaggaaaagt atgcgaaaga caatctaaaa
    14341 accctaatat gccaactggt gatatagaaa tatttgcaac tgaacttaga cttttaaaca
    14401 agtcagaaac tccgcctatt tatataaaag atgatgatga tgtttctgaa gcactaagat
    14461 taaaatatag atacttagat ttaagaaaac catctatgca aagaaattta aaattaaaac
    14521 ataaagtaat gaatattaca agaaattact tatcaaacaa tagattttgc gaaatagaaa
    14581 caccattttt aatagctcca acaccagaag gagcaagaga ttatctagta ccaagtagag
    14641 ttaatccagg aaaattttac gcattaccac aatcaccaca attatataaa caattattga
    14701 tggtaagtgg tatggataga tatttccaaa tagttaaatg ctttagagat gaagacttaa
    14761 gagcagatag acaaccagag tttacacaaa tagactgcga aatgtctttt gttgagcaag
    14821 aagatgttat gagtatgatt gaaggtttgc ttgaagcgat atttaaagaa gttttagatg
    14881 tagaattggc acttccactt cctaagatga cttatgcaga agctatgtct aaatatggtt
    14941 cagataaacc agatactaga tttggatatg agttaacaga tatatctgat gttgtttgta
    15001 actgtggatt taaagtgttt gctgatgcta cacaaccagg aaaaagtgtt agaggtataa
    15061 acgtaaaagg caaagctgac gactttacta gaaaacaaat atcatcacta gaagaacatg
    15121 ctaagacata tagagcaaaa ggtcttgctt ggatgaaagt aggacaagaa ggagtaactt
    15181 ctccaatagc aaaattcttc aatgaagaag aaatgaacgc tatacttaca agaatgaatg
    15241 ctgaagtagg agatttactt ttatttgtag ctgataaaaa ctcaatagta tttgatgctt
    15301 taggacaagt tagacttgaa gtagcaaata ggcttaatct attggataaa aatgtatata
    15361 acttactatg ggtaactgag ttcccagttt ttgaagaaga tgaagaaaca ggtacattct
    15421 cagcaatgca tcacccattt acatcaccta tggatgaaga tttagacaag ttagaagaag
    15481 gtgataagtc atctttaaga gcgaaagctt atgatattgt attgaatggt tatgaaatag
    15541 gtggaggaag tgttagaata tcaaattctg atgttcaatc aagaatgttt aaagcacttg
    15601 gttttacaga ggaaagagct aatgaaaagt ttggttattt attagaagca tttaaatatg
    15661 gtactcctcc tcatgctggt ctagcttttg gtcttgatag acttgttatg ttacttgcag
    15721 gaactgataa tataagagaa gttattgcat tccctaaaaa tcaaaatgct gtttgcccta
    15781 tgacaaatgc tcctacattg gcggaagatg agcaattaga agaattaagt ataaaagttg
    15841 atataaaaga taatgaataa tatgtagttg caagaaacta aaatgtaaaa agtctggata
    15901 taaagtatat ttatattcag acttttttta ttagaattat tttatatttt ttctctatat
    15961 attttcataa attatcctta tattctcatc caatataatt aaaatactat gtttaaaaat
    16021 tttacataaa cttgattgtt ttagaaacta cttctgtaat gagtacacga ttttaattaa
    16081 agaatatata acaaatttga aataagtcta atatatgact taatagatta aattaaaata
    16141 ataaatttgg actatttatc tatttataac ttaaattata tcaaattgat aacaaactag
    16201 atattatact tttttgtaga atagaattac aattatataa agaaaaattc tattaatagt
    16261 gaaaaaaaag tagaaaaaag tagaaattat tcttttataa agaaagggag ggataatatt
    16321 gcaagagaga gaagaattag ttttaaatcc agttccaaaa gaggaaagag ttggatggtt
    16381 agctccattg tttaatatgc ttggttctaa catagctata tctgaactaa tggttggggg
    16441 aactttaatt ttaggaatga ctctttctaa tatgattatt acatcaatta tagggaactt
    16501 aatattagtt gcaatcatta tgatacaagg ttatataggt tacaaagaag ggttaaatac
    16561 atatgtatta gctaaggggg catttggaga gataggagga aagtatctta tatctctatt
    16621 acttggtata actagttttg gatggtttgg agtacaagca ggggttgcag gactatcagt
    16681 acaaaagata ttcccaagtg taaacctaac attagttaca gttatacttg gattatttat
    16741 ggttgttttt gcactatatg gctttaaagc tatggctaaa tttaattact tagttatacc
    16801 accacttatg atattaatgg tgtggggagt ttttaaagct ttttctactt atggagttga
    16861 agctatttat aattatactc ctcaaacaac tatgagtatg gttgaaggtc ttaatattgt
    16921 agtaggtctt gttatagttg gtgcaattat atcacctgac cagttaagat atacgagacg
    16981 agtaaaagac atttggatta tcagtttttt aggtctaggg attatttcat tattccaaca
    17041 agttgcagct ggtgtaatgt ctatgggagc acctacttgg gatataacag aagtattggc
    17101 taatttaggt tttggatggg tagcgtttgt tattttaata cttgcatctt ggtcaacaaa
    17161 cgtatcaaat gcttattctg gtggattagc attaaaaact atttttccaa atgtaaaaag
    17221 aaatgttttg actttagttg caggtctaat aggtacaata atagcagcta ctggtataat
    17281 atttaaattt cagtcatttt tatctttctt gggaatcgca gttcctgcaa tagcaggaat
    17341 tatgtggtgt gagtattatt ttatacaagg aagaacttat aagcatagag aaggaataaa
    17401 ttggattgca gtaataagct ggttaatagg ttttgcagcg tcgtattata gttcaaaaat
    17461 aaatttctta atacctccta taaatggaat tgtagtgagt atggttattt attatatatt
    17521 aatgaaatgt tttggaatca aagataaata aagggggcaa ttacatgggt cgtaaggtga
    17581 aaataggtat aattcaacaa cacagtgtac ttggagatgt taaaaaaaat atagaaaagg
    17641 cagtagagat gatagatgac ttaggaaaac aaggggcaga tataatttgt ttaccagaac
    17701 tatttgctac aggatataat ttagagtctt taggtggagt taaaacatta gaattaataa
    17761 gagaacataa taagtacata gaggaatcaa tgtcagaagc tgctaaaaga aataatgtat
    17821 acctgatatc tccatatgga accttagaaa aagggtctac acatgtttat aattcagcag
    17881 ttatatttga tagaaaaggt aagattatgg gagaatactg taaaaatcat ctgtggtcat
    17941 tagaagcagt atactttaaa ggaggggaaa aagtagaggt atatgatgct gattttggta
    18001 gatttggtgt aatgatatgt tatgatgcag gttttccaga agtatccaga gaattgacat
    18061 taaaaggttc tgaaataata tttataccat ctgcatggag aatacaagat gaagatatgt
    18121 gggatttaaa tgtatctcaa agagctttag agaacactgt atatacagta ggtgtaaatt
    18181 tagtaagtaa tgactctaat ttaatactat ttggaaagag taaaatatgc aatcctagag
    18241 ggactgtaat aactcaatta gatacataca gggaaaaata tgtattatca gagattaatt
    18301 tagatgaaat aaaaaagtat agggaagata tcccctattt gaaagacaga aaaagtagct
    18361 ataacatata attctatact ataatacaag ttcaatataa aatgataaaa agaggatgta
    18421 tcaatattaa gattaaattt attatacttt gatatatcct ctttaatatt atcaactttg
    18481 atttaaatat ttttaaaata gttgataaga aagtttttga acaacaacac agctttagat
    18541 tcatagtttt cgctaaacca tgaaagattt aaatatcttt taaattcaat atctttgata
    18601 tgtaacaact taatctcagg gtcttgagat aatccccatg attttccaca tataaatcca
    18661 actccttgaa gtgattttat aagagcataa attgtatatg gtgaatcaga ctcaaatgct
    18721 attttgggtt tgaaattagc tgattcgcac aggatatcaa ttacctctct ataattctca
    18781 cccttcgtta taactataaa gttttcatct gaaacttcgc ttagatatac ttcatccttt
    18841 aatgataaag gatggtttat tgaaactcct aatagtattt cttcgcatgt taaagttatg
    18901 ctattttctg agtttagctt tgtaaatgaa gaagatatgt acaaatcaaa atcacttttt
    18961 ttataagatg atggcaaagt atgtgacaca ttaaaagtaa tattgggata tagttttctg
    19021 aaattactca aaagtgatgg taacacatta gccgctgcag gagataatat ctttacttct
    19081 cctgaatttt ctaaagaagt gtcttgtaac tccttttttg aactttctac taaatctaaa
    19141 atgctatcta ctctttttag aaacaatctt ccatactcat ttaaaacaat atatcttcct
    19201 tttctgtcaa ataactgaac tccaagttct ttttctaagg aagatatagt tttgcttaaa
    19261 gctggctgtg ctatatttaa cttgtgtgat gcttttgtta tgtgctccat tctagcaaca
    19321 gtttgaaaat acttaagatg caataaatcc atagtataaa atacacctct tttaatatga
    19381 cttgagttat atcaatttta taatcaactc cacattatac attataatat aaagtttata
    19441 taataaacta caatatatta aaaagttatt taaattgtta tttatatttt gtacataatc
    19501 taattaaaaa aagtaacttt aaatgattaa ctaaggaaat attgttacaa actttagtta
    19561 tataaaaaat tcatttgatg agcaaagtat taatgtcaag taagaataaa agcttattag
    19621 ataagaaaaa taatatttta taataatgat gttacttaaa ttattatgtt atactatttt
    19681 aaataggaaa catttagata ctataatttt agggggagtc aagatgaaat taactaaatc
    19741 aatatcatca atgctgtgct tatctctagt tttaagctta tcaccagtag catatgcaaa
    19801 tgaaataagt agaacaacag ttgagaatac tttagtaggt caggataggt atcaaactgc
    19861 tgttgaagta agtaaaaaag gatggagtag tgcaaatgag gctgtagttg taaatagtga
    19921 atctattgta gatgcattag ctgtaacacc atttgctaaa ttaaaaaatg ctcctatatt
    19981 attgacagaa aaaaataatc taaattcaca aactgagaaa gaattaaaaa gattaggagt
    20041 taaaaaagtt tatataattg gtctatctag ttctatatca aatgatgtgc aaaaacagtt
    20101 agaatctaat aatttaacta ctgatagagt tggtggtagt gatagatacc aaacagcttt
    20161 gagtatagct aaaaaaatag aaggattaaa agatatatca gaaatagctg tagtaaatgg
    20221 gtacacaggt cttgcagatg cagttagtat agcttctgta gctgctacta atggaatggt
    20281 tatacttcca gtatctgatg catctggtat atctagcttt aaagatttta tagaatctaa
    20341 gaatataaat aaatcataca taataggctc tactaatgct gtatcagata aaataaaaca
    20401 aagtttacct aattcagaaa gaataggtgg agcagataga aatgaaacta atggtaaagt
    20461 aatagaaaag ttttatacat ctaataaatt aaacaatgta tttgtggcta aggatggaat
    20521 gaaaaatcaa aatcaactta tagacgctct agcagtaggt gtactagcat caaaagaaaa
    20581 ttctcctgta ctaatcgtag gagaacaatt aagtgcaaca caaaagagtg tattttcgaa
    20641 taaaaatgct acctctataa ctcaagttgg tagtggagga aatgaaaatg catttaaaga
    20701 attaaaagat ttacaggcaa atagtaacaa taagagagta atgtatgtaa ctaatacaga
    20761 taaggtaaat ataagaaatg atgctacaat agaagcttct gttataggat atctaaataa
    20821 tggtgatgaa gtagaagttt tagatgtgtt aaaaactgga tgggtaaaaa taaaatacaa
    20881 tgaaggtatt ggatatgtta gtgggtctta tttgacaaat aataagcctg ataatagtaa
    20941 tgaaaatata aaaataaagt atgtaaaaga aaaagatggt ctaaatgtta ggaaaggacc
    21001 aagtacagaa gatgaaaaaa taggtcacct ttcatatgga agcaaagtag aaacaataga
    21061 aatgtttgct actggatggg taaaaataaa gtataatggt ggatatggtt atgtaagtaa
    21121 tgattactta tctgatacac cagttattta attataagta acaataaaaa actgctttaa
    21181 tttgtaaagc agttttttat aaaaatttaa gtctaataac tatttctatt ttgatacctt
    21241 aatcctggga tttattatta tggatattat caacatacaa acactccaaa tccagtgtct
    21301 aattccaaac ttagatatta ataaattcag tcctgctatg gtaaataagc agtatttaat
    21361 tacaattaaa agtgtattta tagaaagccc cttcatagaa agtatcctaa atccaataat
    21421 agacattcta agtcctataa atgccaataa tatacctgca atatcctgaa ttaagtaata
    21481 gtattcaaat ttcatattca atcaactctt ctctgagcag tatcaaatat ttctaactct
    21541 tcaatttcat catctgtgta tgtaagcata gtaaaaccag ttacaccata aataaatgaa
    21601 attataggtg taatccaact taagaaacaa tatggaataa attctagtgg gcttacacca
    21661 agagtctgag cacagaaaac agcatttgaa ttccatggta ttaatggacc accaagtgta
    21721 gctgtatctt ctataattct agatagattt tgtggtttta atttaaattc tttgtatatt
    21781 ggagacatta gagttccgac aaatacagaa gtgaacatca ttgatgaact aaatgcattt
    21841 gcaaagtagc ttactataaa agtagaccca accaatttag ttcttgattt tcctatttta
    21901 gttataagag gttgaagcag tacttctaag aatccagttt tttggagaat accagcaata
    21961 acgaaaacta caaatactag taaaacggtt tcagccatac tcattattcc accacgattt
    22021 aatagtttgt cggcatagac aaatccagta tctatagtaa atcctgatag catgtaattt
    22081 aaaacatcac ctattttttc tccttcttga aatactgcta taataagtcc catgatagct
    22141 gaacttagta tagatataat gggtggcttt tgtagcaata aaagtaataa taaaaatatt
    22201 attggaatca ttgctactaa accaatatga aagtttgaat ttaatacatc cttgatttga
    22261 tttatttgaa catagtctat agtattattt gaatacttaa atccaattac tgtatacaga
    22321 actatacata ttacgtatgc aggtcctgtg gtataaaaca tatgcttcat atgtgtaatt
    22381 acatctgtct tacaaactgc tgcagctaag tttgtagaat ctgatagagg tgacatctta
    22441 tctccaaaga aagcaccaga aatgactgca ccagctgtta aaccaagagg aatacccata
    22501 ctagtaccaa tactcatcat tgctatacca gaagttccaa gagtccccca agatgtacca
    22561 gtagcaacag aagttaatga acatacgatt aatgttgtaa gcagaaaata tttaggggtt
    22621 attattgtta gtccactata tactactgtt ggtattgtac cagcagctat ccaagctgct
    22681 attagtacac caacagaaag tataattaag tttgattgaa aggcatcttt accaacttca
    22741 aatccaaaag cttctatttc atcattagta taaccaagtt tcatggctgc aggaactact
    22801 ataagccatg aaagtaaaaa cattgtagtt atagaagcat taaatacaac tataccaact
    22861 gatgttagag ctattattgc aagcattact aacaaggaat atctaaatga aggtattttt
    22921 ctttctgtat tcattttcat agtgcaaatt ctccttaaat atttttagtt aattgcattt
    22981 tttattctta taagagcttc ttcaagcata cttcttggac aagctaagtt tattctttga
    23041 tacccacttc ctccaatgcc aaagctgttt ccttgattta gagcaacttt acctttttgt
    23101 acaagtatac tttctagttc ctcatcactt aatccaagtg cactaaagtc tacccataat
    23161 aaataagtac cttctggttt tctaacttta agttttggca tattttcatt tatatatttt
    23221 atagcaaaat ctatattaga ttctaaatat tctaaaaatg attctagcca agattcacca
    23281 ttattatatg aagcttcagt tgcaactaag ctaaagcaat tatttctctt tatatcaatt
    23341 ctggtaaaag catcatctaa caatttatag tctttttcat caggtagtat tacatatgat
    23401 gattgaagac cagctatatt aaaagtttta gttggagcca tacaagtaat tgtatttttt
    23461 tcaaattctt tagaaataga tgccatagga atatgcttat gtttttttaa tataatgtca
    23521 ctatgtattt catcagatat gatttttaca ttatgcttaa gacaaatatc tcctagtttt
    23581 tttaattcat cttttgtcca tactctacca actggattat gaggattaca aagtatgaat
    23641 aattttacat ctttaatctt gttttcaatg tcttcataat ccattatgta gtttccattt
    23701 tctagttttt gtaatgggct tataattagt tctctattgt tattttttac aacactatta
    23761 aaagggctgt aaactggttc ttgtatcatt attttatcat ttgcttttgt gagttcattt
    23821 attaaaagac ttatagctgg gataacacct ggactataaa ttaaccactc actttttatt
    23881 ttccagttat gtctcctatc tagccagttt actatagatt cattgtaaga atctggtctt
    23941 gttgtgtagc cataaatctc ttgttctagt ctatttttta aggaatcaat tatacaaggt
    24001 gcagccttaa aatccatgtc agccacccac ataggaagta aatcattagt tccatacttt
    24061 ttttccattt ctgaccattt agatgaaaaa ttattgcttc tatcaacaat ctcattaaaa
    24121 ttataattca ttgtaaatac ctccagtaat tttaatttta tgttaataat attaagcaaa
    24181 caacgtgcca atattgataa taaaaaatat ttatccacaa gttaatattt aatattaagt
    24241 aatttcagag catagataaa ataaaaatat taattatact taaaactttg tggaaaaggt
    24301 tatttctaat aaatttttaa atatatgaaa atgtaattaa ataggttaaa taggtttaac
    24361 tgtttaactt gttaaaccta tttatatttt ttagaaattc tactcctttt gatgttataa
    24421 ttgaacctcc acgaccaaca agtatgtcta caagcccaaa gtcttgtagt tctaaaagca
    24481 tatttctaat ctgttgttct gtcaaaaatc tattttcctc taaagctatt ttacataagc
    24541 tttttctacc tattctttct tttaaatcat aagccttttt taaattatcc aatataaata
    24601 tgtattcatc tagtgacctt ttaaaattat agtcgtattt catgatgttt ttgtctttgc
    24661 caatgttgaa ttgatacttt tttatattat cacttacttt attacaaacg gttctactat
    24721 aatttgaatc tatagtatcc aatatatatt caggtaaatc acatatttct ataatatctt
    24781 tttccaagta acagaagtat tctcctaagt ttcttaattc tcttacattt ccttcccatc
    24841 tatacatttt aaagatttct tttacttcct cacttagaat aaaattacat ggaatatcat
    24901 attttaatga accaaaaatt tcaaatatat cagcaggtct ttccctaagt ggatgtattt
    24961 taaggggaag aacattcaac ctaaaatata aatctcttct aaacatattt ttagatacaa
    25021 gttcttttaa atttctatta gtagctgcaa ttattcttac atcaatgtca atgacattat
    25081 tagaaccaat acgtcgtact tgtttttctt gtatgactct aagtaattta gattgagaat
    25141 ttaaatccat ttctccaatt tcgtctaaaa atatagttcc attgttggct aattcaaata
    25201 aaccaatttt cccacctttt ttagcaccag taaaagcccc ttcatcatag ccaaataatt
    25261 cactttgcag tagattttct tgaaaagtac tacaatttac tgcgacaaaa ggaccatctt
    25321 ttcttctaga agcactatga atggattgag caaataattc tttaccagta ccactttcac
    25381 caattataag tatagatgaa tttgattgag ccattttatt agcaattttt tttgtattaa
    25441 tagtttgtat actagaacct aatatatcat caaatgtgta tttactgata ttaccagagt
    25501 ttactagttg tgctcttaat ttagcttgct ttttttctaa ttgagaaaac tttgtcatct
    25561 ttagtataaa tccatcaaat acatttaatt gtatatgttt aatctctaaa tttatatcaa
    25621 tatgatttat tttaatcaat ttctcaaaaa atggagtttt attggtcaat atgctttgaa
    25681 aattgatgtc ttttatgcaa tcaccaacaa atgagtcaat catttcattt gcatttatag
    25741 acaaaatttt tctagccata tgattataga attgaattat tccatcatta tttgtgcata
    25801 taattccatc atcaattata gatagtaaca aatcaaattg tctttcaaac ttatttgcat
    25861 ctaacaataa ctcacctgta ctataacttg taggaattat tttttctacg tattttttta
    25921 ttaggtcatc ttttattaga tgttttaatt tggtttttgt agcaatttca acaatacaac
    25981 ttaaatctat tattctgtat tttaaatcta taacttcaca atttttaaac ttaggcaaga
    26041 ttttttcgcc aggagttagg attacagcat ctgtgggagt aaactcaatc tctggatagc
    26101 agggcaggat atttatattg tctattccta atctatgtat taaagaaatt gtttctattg
    26161 ccatatcttt acttaagtta tatacataaa taattttatc tttttctaat ttggatatca
    26221 tatcaatact gttcttttca aatgtcaact tgggaacaat gatttgagca tcatagtgag
    26281 ataattttac tatttcatca tattttaaat aagcagatag gagtataagt ttttctttaa
    26341 aaaactttaa atttccctct tcaaatgagt ataaatttat ctttagatta tcacctaaga
    26401 attgccttat ttgattttca taaatttctc cagcgtcttt ttttaaactt acaatagcta
    26461 attccatttg tttcatttta acctctcata caaatttgac ttattttaga ttacataata
    26521 attaaattaa ataatattat ttaattatta ttataaattt attatagact acacattgaa
    26581 gtaaaagaag tagctaaaat tattcctttt atttttgttt tttgtatcta tttggtataa
    26641 tagtattaga gcgtataata atttaggcaa aattttttaa ggaagactag aggagaatat
    26701 aaaatgaaaa aatgtcttta ctgtaatagg gatataaagt caccaaataa taaattagct
    26761 ataaaatatg atgacaatat gaatatatat ccatgtcgtg cagaatgtaa ggaaaaaatt
    26821 gaagaatata tagcaaaaaa acctacatat aagtttataa atatattaga ggttctttta
    26881 ggtgtgggcg gaatattctg ctttttgagt aaatggacta ttgcagccca agttgtactt
    26941 ggaattgatg cactggttat atttttattc ccattggcag gatttaagat gaatggaaag
    27001 ttaagtatgg aacaaactaa gaatcaaagt aggtctattg caatatcaat tttggcattt
    27061 atagtaataa taactgtatt atattttaaa caagttggta agtagagcat aattgtataa
    27121 gacatcatta gagtatgagg tgagtttatg gaagaaagaa aattagcacc aaacaatgag
    27181 agggaagctc tcataaaaag attaaaaaga atagaaggtc aagttaaggg tatacaaaag
    27241 atggtggaac aagaacgtta ttgtgttgat atattggttc aaatatctgc tataaggtct
    27301 gctataaata gagtaggcac aattatctta gagaatcata taaaaggatg tgtagcagaa
    27361 agcattaaac atgatgatgc tgataaaact gaagagatga taaaagagct tatggatact
    27421 ataaataaat ttacaaaata aggaggcggt tagttatgaa tcaacaagga tttattatgg
    27481 aaattgttga tgatagaact gcaaagttaa aaatgcaaag acattcagct tgtgctgctt
    27541 gtggaaaatg tgctactaca aattcagcag agtcaaaaga aatattagta gaagtagaca
    27601 acacaattgg tgcaaaagtt ggtgaccatg tagaagtgag tatggataat atgaatgtat
    27661 taaaagcagc tgtaatggct tatataatac ctcttatagc acttcttgta ggaacaatag
    27721 ggacttatta tatattgggg gcaattggaa tgacaactgg agttgaagcc ataagtggat
    27781 tagttggaat ttgtcttaca ataatctgtt atttatactt aaagaagaat gacaaaaaat
    27841 ttagagacag tagagaattt ataccagtaa taacaagaat attaattgac ctttaagata
    27901 attataatta aaaaatagca atgtgctttt taaataaata ctcaatacgt atttgtagag
    27961 tttaagtaca ttgctatttt tatattttta ttttgatttt ctgtaaggaa taaagttttt
    28021 tctgattttc aaaagattca atgttcttac aaaagaagtt acaactaata caccaagtgc
    28081 tattgcagca cttagaaata gcgcatctgt accttttgac atagccaaag aataattttt
    28141 ctgaatcaaa gacaacattg tattatatag cccaattcct ggtattagag gaagaatacc
    28201 tggaattacg aacacaatag ctggttgctt tagttttctt gctaaagtct cactgcacgc
    28261 tgagaccaaa gcagcagcga taaaacctgc aaaaactgca tttgtagtaa aattaaataa
    28321 ataaacatat acaatccaac caattccgcc agttactcca gctggaagaa gtagatatag
    28381 tggagcatta aagaaaacag caaatccagc agttgcaaca cctgaaaata aaaaatgcaa
    28441 aatcatagac atatcagtca ttagaaagga cctcctattc tataccatgt atcaaggaca
    28501 aaaccaactc cagaagcaat tgcaataata atcatagcag catcaaaagc acgagaaatt
    28561 cctgatatta aattccctga tattaaatct ctaatagctt ttataaaagc aactcctggg
    28621 agtagtggca ttatagcgcc gactataagc attttagggt catctaaaat gcctatttca
    28681 actagtagaa ctcctgaaag tgcaattaaa aaagaagata ctaaacagga aaatgcagat
    28741 attgcactta tcttcatcgt tttatcataa gtaattgcag caaagattgc tattataaac
    28801 gtaagtataa aatttaatac ataattccct ccaagcaaac aagcaaaaca agcagatgca
    28861 attcctgtac aagagtaaaa taaccataaa ggataaggtt ttacatcttg gatttcataa
    28921 agacgtgcaa tggcattatc aatatcatac tctttgtcac taacaaattc tcgagaaaaa
    28981 ctgtttaata aagaaatctt atttaagttt atacctcttg attgaatcgt tttcataaac
    29041 gaaaagccat caaatttctc atctgaaata attattactg taggagttgt aaaaatattt
    29101 acatgataaa accctcttga cctgcatatt ctaaggacac tgtcttcaac cctagatgtt
    29161 tcagctccat tttttatcat cagttcccct acaaatagag ctagttttag aatatttttc
    29221 ttatattcaa gttcctcatt ttgattcata aatacacctc caaattaaaa aacgtaatat
    29281 tgaaattata tcaaattttt aagtaatatt ccttaatatc tatataaata tattatcttt
    29341 tttttgtttc aaaatagtca catttaaatt atatccaatt aaaagaatta gacaatatta
    29401 tttattatat tttaacttaa aaataaagaa agtaactatg ttaaatatgg aaaaatggca
    29461 aaacacttga ctattaattg gtaaaggact ataataataa attatcatat tgataaaaac
    29521 gaaaattatg aaatgtctgt aaaaataaaa tttaaataat aactaggagg agtaatatgt
    29581 ttgagaactt gaaaaaagcg gaccctgaga tttatgaaag tatgaaaaga gagttaaaaa
    29641 gacaacaaag aaatattgaa ttaatagcat ctgaaaacat agtatcagtt ccagtaatgg
    29701 aaactatggg aagtcatctt acaaataagt atgcagaagg ttactcaggc aaaagatatt
    29761 atggtggatg tgaatacata gatgaagttg aaactcttgc aatagataga atcaaaaaaa
    29821 tatttggagc agaacatgct aatgttcaac ctcactctgg agcaaatgca aatattggtg
    29881 tatattttgc tatgttaaaa ccaggtgatg ttgttatggg aatgaattta tctcaaggtg
    29941 gacatttaac tcatggtgct ccagttaata tttctggtca atattataaa ttttatgaat
    30001 atggtataga taaagacagt ggacttatag attttgatga agttagaaag atagcacatg
    30061 aagtaaaacc taaaatgata gttgcaggag caagtgcata tcctagagaa atagatttta
    30121 aaaagtttag agagatagct gatgaggttg gagcattgct tatggtagat atggctcata
    30181 tagcaggtct tgtggctgct ggtcttcacc aaaatccatg tgaagtggct gattttgtta
    30241 caacaacaac tcacaaaact ttaagaggac ctcgtggggg tgttattctt tgtaaagaaa
    30301 aatatgctaa agacatagac aaagcaatat tccctggtat tcaaggtgga cctcttgaac
    30361 atataattgc ttctaaggca gtttgcttta aagaagcttt aagtgatgag tttaaagaat
    30421 atcaggtaca agttgctaaa aatgcaaaag ccttagctga agaacttatt aaaagagatt
    30481 ttaaattgat ttctggaggt acagataatc atttaatact attagatttg actaataaaa
    30541 atataactgg taaagcagca gaaaagagat tggatgatgc ttatataaca gctaataaaa
    30601 atacaattcc atttgaccca aatggtgctc ttgttacaag tggaataaga cttggtactc
    30661 cagctgtaac tactagaggt atgaaagaag aggatatggc aataatcgct gaggctatag
    30721 acctatgttt aacttatgat gaagaatcta aggctagaac attggttgtt ggtttaactg
    30781 aaaaatatcc tttatatgaa gaatataata ttatggatta attataaaga acaactaaat
    30841 aaaatacatt taaataatta caatgattgt gtatgtattt gaatatagtt agatataaaa
    30901 aaggtgtctc aaaatgaact ttttagttta tgaggcactt ttttgtatgg aatcgaacat
    30961 atttgtgtta tttaaataat aaaaaatatg gttatctcta tttttagatg gtctctttgt
    31021 attttttaat taaaacttta agatttctta agaattatac taggtttttc taaagattta
    31081 aagtctatac ttataagtat agtaatcata tgaaaagaaa agctttaacg aggagggaat
    31141 cttatgaagg tattaattct tacaggaaaa tttggtatgg gtcattactc agcatcaaat
    31201 tctttaagtg aagatataaa agcaaaattt gataattcag aaataataat taaagatata
    31261 tttgagtaca ttatgcctaa ctattcagat aaaatgtaca agactttttc tattcttgta
    31321 aatcggggaa gtagtcttta taatttattt tataaatgtg ctgaaaatgg aaagaaagat
    31381 ataaaattta ctttttcaga ttatttttta aataaattag atacattact tcatgaagta
    31441 caaccaacag tagttatttc aacttttcca ttttgttctc aattagtatc aagatataag
    31501 gaaaaatata actcaaatct tcctttaata acatgcataa ctgatataag tagtcactca
    31561 gaatggatta gtaaaaatac agactgttat ttagtagcta gtaaatctac taaggaagaa
    31621 cttgttttta aaggaataga tgaatctaag attaaagtta atggtattcc agtaaaaaaa
    31681 gaatttaaaa gaatagaaca tgtaaatcat tcaactaaaa aaaatattct tataatgggt
    31741 ggaggattag gattactgcc taaatcagaa caattttaca aagaattaaa tagtttagaa
    31801 ggagtaaaaa ctactgtaat aactggtaat aataaaaaaa tgtactataa actatatggt
    31861 agatatgaaa atatagaggt agtaggttac accaatgaag tatataaata tatgaaagat
    31921 tctgacctta ttatttctaa accaggtgga ataacactat ttgaaacaat ttattcagaa
    31981 cttccaatat tggcatttaa tccattttta caacaagaga tagataatgc tagttttata
    32041 ttaaataatg aaattgggag aatacttggt aaaaataaaa agtactatgt agatgagata
    32101 agagatttaa tttatgatga tgctactctt aaaaaaatga gcagtaacat gaaagaatta
    32161 aaaaaacaat ttgataataa taccttagag aatattttat tttcacttga tgaacaagga
    32221 gcgtgtagag aatgtatgta gtaggtttaa ttatcgttgt tgcattgatt tttttagtcc
    32281 attcaattat acctacatac tataataaac tattaaataa agaggtatta aaaaatatgg
    32341 ctggagaaaa tgaaattgca cttacatttg atgatggtcc agataagaga tatacagaaa
    32401 aattactaga tgtgttaaaa gaaaatgata tacaagctat gttttttgta gtagcaaaga
    32461 atgctgaaaa agagcctgaa attataaaaa gaatgttgag agaaaatcat atagttggtt
    32521 tacattcatt ggaacacaga aatgcatggc tttatagtta tagttatgtt aaaaaagatt
    32581 ttattgaaag tacaaatata atgaaaaatt taggtgtaga tgtaaactac tatcgtccac
    32641 catggggtca tactaatata ttttcaaatt catttgttaa aaaatataat ctaaaaatga
    32701 ctttgtggga tgtaatggca gaagattggg agaaagatag tacagtagac attattataa
    32761 acaagttaat gagtagaaca aaagaaaatt ctataatttg tttacatgat gcaggtgaaa
    32821 attcaggtgg tgcagtaggt gcacctgagc gtactattga agctttaaag atagctattc
    32881 caaaacttaa agccagtggt ttaaagttcg taacaccaga aaggatgtag ttatgataag
    32941 taaaactgaa aagaaaaata aatgcctagg tagtgtagct tttttagttt tactaatggg
    33001 cataacagga tattttgttt ttagagggca gtcagttgaa tccttgataa aatcattaaa
    33061 aggtgcaagc cctatgttta tactaattgg atttgcaatg atgtttatat atgttgcctg
    33121 tgaaggtata aatatatact taggtatgaa agctttaaac caaaagacta ctcttttaaa
    33181 atgtatggga tatgctttta ttggatttta ttttagttct attacgcctt cagcatcagg
    33241 tggtcaacca gcacaagttt attatatgaa aaaagacgat ataaatatat catattcttc
    33301 acttatacta ttagtcattg ttgttattca ccaagtagtt atactggcat atagtggaat
    33361 aatgttcatt atggaacgtg agtttatatt aaataatgta agtggtatga acattttact
    33421 aatatatggt gtaataacaa atgttgctct tgtcattgga gtaattgcaa ttatattttc
    33481 aaaaaaactt gtaaacaatt ttataatatc aataacaaat ttattaggta agttaagaat
    33541 aataaaagat gttgaaagtt caagaaaagt tataatatct caaattgaag agtatgttaa
    33601 aggtgctcaa tatataaagc agaatccaaa acttgtagtc caaattttag tcataactat
    33661 tgtacaaata acagctatgt ttttagttcc tttctttgta tacaaagctt ttcatctatc
    33721 aacatatact gtatttgaga tattagcgat acaatccttg cttaatattg ctgtttcttc
    33781 attaccattg ccaggggctg ttggagcttc tgaaaatagt tttatgacat tatttaagat
    33841 atttttccct ggtcacttat tagttccggc aatgttactt agtaggggaa taagttttta
    33901 tgcatttgta gctataagtg gattgatatg tatagtagta catgtaaaat cttctgaaaa
    33961 agtaaaagca gtgaaaaaag ttgcttatgt aagataatat tatatagatt atttttatta
    34021 atatgtcaat ttgtgtgtat attttaatta agctttaaac atgtttacag gagtagaaac
    34081 tattaaaacg tgtgacattg ctaagctttt gctaataaag tataatattt tatatagttt
    34141 gaaatattta agaaatatag caaaaaaagg agcttggtga tgaataaaga gattccaaaa
    34201 aaaattttag tagctgatga taatcctgag attagggaaa tcgtagaaat acttttaact
    34261 ggtgaaggtt atgaagttat tatggcaacg aatggagaag aagctgtaaa cttagtagat
    34321 agtacgattg atttgattat tttggatgtt gtaatgcctg taaaaacagg atttgttgca
    34381 tgtaatgaaa ttagagaaaa aacgacagca ccaatattat ttttaactgc aaaaactcaa
    34441 gactctgata aagttattgg attttcagca ggaggagatg actatttatc aaagcctttt
    34501 tcatattctg aactaatttc aagggttaag tcgctactta gacgttatta tatataccag
    34561 ggaaaggaaa aggaaagaga gccaaatata ttaaaaacaa aagagttatg tgtcaattta
    34621 gatactcaag atgttacact aaatggagaa cctgtgtttt tgacaactat agaatatagt
    34681 atattaattc ttatgttgaa aaatagaaaa aaagtttttt cgtctgaaaa tttatatgaa
    34741 actatttggg atgagcagta tttttatacg gcaaataata caataatggt tcatattaga
    34801 aatttaagaa aaaagttaga aaaagaccaa aaaaatccac aatatataaa aaccgcatgg
    34861 ggaaaggggt attatattga ttaacaaaat taagccatct aagttgggag ttatactatt
    34921 gatatatgta gtaatctcag ctgcagtggc tttcatttta aatataccat tttactctgc
    34981 tgttgaatct atagtaaaag atgtatcaga agggaaagta tattttaaaa gagaagtaaa
    35041 tcaacttatg gataattttc aaacttatgt gaataacaat ggtataagag cagatgatgg
    35101 agatgatata gcaaaatgga atagtgataa ttggtttgtt tctatgaaga tatataaagg
    35161 aaataaggtt ttttatgata cattatacta tcctaatgaa gtacctgata agagtgagga
    35221 aaatgagttt tatagcagtc ccatggctag aaactatgag atacaatttt ctgatattaa
    35281 agctaatata gttgtagtag ctttatttag atttagattt caaaatttta tagaagcaat
    35341 ttctattaca tttatggcac ttacatttat tataacattt ttactgttgt ttagtagaaa
    35401 gataaagtac ctaaggaaaa tagaaatggg tataaagata gtagagagtg gcagtttaaa
    35461 gtatagaatc ccagtaaaag gtaatgatga attatcatca cttgcatcta gtattaatga
    35521 gatgagtcat acattggaga atcaacttat aaaagaaagg gagatgaaga ataaaaataa
    35581 agatatagta acttcaatat cacatgatgt tagaactcca ttaacgtcag taatttgtta
    35641 tttagatttg attaaagacc ataaatacaa tagtcctgag caattggaac aatacataaa
    35701 aaatgtaagg tctaaagcat atcaaataaa agatttaaca gatgaccttt ttacgcattt
    35761 tgttaattct ggagaggaag tttcaaaagg gtatgaaaaa ataaatgcta atgaatttat
    35821 ttttcaatta cttttagata gttcatatac attggaggag aaaggatttc atataacaat
    35881 agaaaatgaa ctttataacc aatatcatat ttatgtagat gttacacaat ttagaaggat
    35941 atttgataat ttatgttcta atataataaa atatgctagg aaagataagc ctatagtatt
    36001 tttaattaaa gtagaggatt ctaaattact aattatacaa aaaaatagag taagtaattt
    36061 ttctaagaat gtagaaagtt ttggaattgg tataaagaat tgtgagaaaa tagttgcata
    36121 tcataatgga agcataaatg taaattcaga tgataaagaa tttgctattg aaatatctat
    36181 tccagcaaaa gaatcataaa aatttaaatt atatatagtg taattgctat tatatatagt
    36241 gtaattgttt atgtaaaatt ctatgaagca ggaagcatgt gactttagtt atgggatgtt
    36301 caatattgtg tacctctagg caagtaacaa gtttacttac aagaccatat actattatta
    36361 tcttaaaaaa aggggacata ataatatggt tttgacagga gcacattata tttacattct
    36421 atttatggta attatattaa ttactatgat tatgaaaaag gatacaatag taccatgtat
    36481 attaggtgtt ttttttatgg gattgttctt tgaaaaaaac atttttggtg ctataacagc
    36541 agtgtttaac tcttttatta tatctttaaa tgagttgggt ccaattatct tgattatagc
    36601 tattatggtt gcattatcta aagccctaga agccaataat gctatacagt atatggtgag
    36661 accattttcg agagttataa aaaattctaa tacagcattt tttgtaactg gatttgttat
    36721 gttagttctt tcttggtttt tctggccaac tccagctgtt gccttagttg gagctgtatt
    36781 tttacctgtt gctatgagag ctgggctacc agcaattggt gttgcagtag cattaaattt
    36841 atttggtcat ggtcttgcct tgtctacaga ctttgttatt caaggagctc caagtataac
    36901 agctggtgca gcaggtgttg cagtatcata tgttataaat gatggtatga ttttgttttg
    36961 ggtgatggga atcgtaacca tatctatggc attttataca ttaaaaagag atattaacaa
    37021 aggaatgttt agagaagaac taaaaggttt tgaaagtgaa gaagtagaag agttcaatgg
    37081 aaaatctaaa atagcaacta ttttagtagc tctgggattt cttgctgata taattgctat
    37141 gtatatattt gacctaaaag gaggagatgc atcagctctt ttgggtggaa cagcagtatt
    37201 tttaataata attataaatg ttataaattt tggcaaagat agtcttgaaa atgtatgtga
    37261 gaatataatt gatggttttg tgtttgggat aaaaatattt ggagcaatta taccaatagc
    37321 agcatttttc tatatgggtg aagttgcacc attgacaggt gtatttggaa aagtattagc
    37381 accaggttca caaggtttat tatcagatat aggcatagct ctttctcaaa caataccact
    37441 taataaattt gccgtatcag gtatagaaac tgtagtggga gcaataactg ggcttgatgg
    37501 ttctggattt tctgggattt cattagttgg ttcacttgcc tctgtatttg gaactgctat
    37561 aaatgcaagt gttggtgcat tagctgcatt aggacaaatc agtggtatat gggtaggtgg
    37621 aggctgtcta gttccctggg gtcttatttc agcagcggca atttgtggag ttagtccaat
    37681 agaattggca aagcgaaact ttgtcccagt tataaccgga ttggttgtaa caaccatagt
    37741 agcaatgttt attatttaat aaatcgaata aatctttaat tgtataaatt aaaaatagat
    37801 gtgtagctat aatttattat aattgtttta ctaattctaa gccatacatc tatttttatt
    37861 gatattttta ttagtattct ttaataaaat aatatttttc attatactat acataacatc
    37921 taaatataac atctaaaata ctgttatcta tatttaatat cttgccaaat tttttgatgt
    37981 tttattgccc tgtcccacca acatatataa gctatcacaa gtttttacct atactatcac
    38041 caaacaaaaa ttctaatttt aatttattat tttataacct atattatatt aactatatta
    38101 gtttgtagac tttagatgta attataaata ttaatactcc aagagttcaa attcatatcc
    38161 tagttttctt gcatttgata taaaatcatt taaaatttct gtactagttt tggaaactgc
    38221 atgtagtaac aagatagaac catcatgtag attatccatt attttttgcg ttgcttcttc
    38281 atgtgatggt tgtttatcag tatcccagtc tctgtatgca aaactccaaa atacagtctt
    38341 gtatcctaaa tttttagtca ttgccaagct tttttctgag tattttccca taggaggtct
    38401 aaaaaattta accatatctt tcccagttac atctttatat aacttttcaa catcatataa
    38461 ttcgtcatta aagtttttta gattgcttgt ttttgttggc atagatggat gggttttact
    38521 atgatttcca actatatgtc cctcagaaac cattcttttt actaaatctt tattttcttt
    38581 tatatatgga gctgtaacaa aaaaaactgc tttaacctgg ttttgtttta gagtgtcgag
    38641 tatctttgta gtgtatccat tttcataacc ttcatcaaat gtaagatata gtgttttttg
    38701 accatctttt gtaggtccat tgtatagggc atcatagtca gaaaatttaa attcaatgtc
    38761 cgtatttact tctggtgttt tatgcttatt gtttggtata taaaaccaat caagagtagt
    38821 gttatctaaa gaagatacat ctaatttatt attatcatta ttagtagtgg cttttgatgg
    38881 tttatctgtt ccgtctgaaa ctattacatt ggaagtatcc ttaccactat ctattttttc
    38941 cttatcttct tgtaactgtg tttccttatt ttgattttcg ttttgattgt tttgtgaatt
    39001 tgaacatcca acaataaaaa aaatagaaat agaaagtagt gttaaataat gcaatacctt
    39061 tctaaacatt ttgaagctcc tttctaaatt aataataatt gcatataata ataattatat
    39121 gattaattac aattgcatat agtattatta taattggaaa ttaccattaa taatagttat
    39181 atctttaaaa ataaagtcac aatagtatga caaagggtga ataatcaata aatttaaaaa
    39241 aaaatgaaaa aaatagttga ccatattatg agaagatgtt attataataa atgtgctcaa
    39301 caagaagtaa caaaataaca agcacaaaga agttaattga at
//
LOCUS       DAEMHW010000014        38503 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEMHW010000014 DAEMHW010000000
VERSION     DAEMHW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.014"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..804
                     /locus_tag="KPK67_000610"
     CDS             <1..804
                     /locus_tag="KPK67_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457617.1"
                     /translation="PYLDPANYADYITNEEIPQDELRNDPQISNGYTKIPNNQNINNL
                     YPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNNNTGMPNNMSNNMNS
                     NMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNMGMPNNMSNNMNSNM
                     GMPNNMSGNMNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCNQNMPPNVLMMIPGVI
                     CHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEEFDEEEM"
     gene            complement(1241..2329)
                     /locus_tag="KPK67_000611"
     CDS             complement(1241..2329)
                     /locus_tag="KPK67_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457618.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            2454..3182
                     /locus_tag="KPK67_000612"
     CDS             2454..3182
                     /locus_tag="KPK67_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG2457619.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(3384..3875)
                     /locus_tag="KPK67_000613"
     CDS             complement(3384..3875)
                     /locus_tag="KPK67_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457620.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(3886..4329)
                     /locus_tag="KPK67_000614"
     CDS             complement(3886..4329)
                     /locus_tag="KPK67_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457621.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(4319..5158)
                     /locus_tag="KPK67_000615"
     CDS             complement(4319..5158)
                     /locus_tag="KPK67_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457622.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNISIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(5377..6762)
                     /locus_tag="KPK67_000616"
     CDS             complement(5377..6762)
                     /locus_tag="KPK67_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2457623.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELRD
                     TVGSFKYIPII"
     gene            complement(6935..7678)
                     /locus_tag="KPK67_000617"
     CDS             complement(6935..7678)
                     /locus_tag="KPK67_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBG2457624.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETAHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(7698..8960)
                     /locus_tag="KPK67_000618"
     CDS             complement(7698..8960)
                     /locus_tag="KPK67_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2457625.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGTGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPLPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(9144..10142)
                     /locus_tag="KPK67_000619"
     CDS             complement(9144..10142)
                     /locus_tag="KPK67_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBG2457626.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGEVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(10126..11637)
                     /locus_tag="KPK67_000620"
     CDS             complement(10126..11637)
                     /locus_tag="KPK67_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG2457627.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNILVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(11691..12653)
                     /locus_tag="KPK67_000621"
     CDS             complement(11691..12653)
                     /locus_tag="KPK67_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG2457628.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPNQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(12701..13591)
                     /gene="rbsK"
                     /locus_tag="KPK67_000622"
     CDS             complement(12701..13591)
                     /gene="rbsK"
                     /locus_tag="KPK67_000622"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBG2457629.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIKVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(13633..14646)
                     /locus_tag="KPK67_000623"
     CDS             complement(13633..14646)
                     /locus_tag="KPK67_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2457630.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(14875..15852)
                     /gene="bioB"
                     /locus_tag="KPK67_000624"
     CDS             complement(14875..15852)
                     /gene="bioB"
                     /locus_tag="KPK67_000624"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBG2457631.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFKNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(16058..16321)
                     /locus_tag="KPK67_000625"
     CDS             complement(16058..16321)
                     /locus_tag="KPK67_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457632.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(16391..17464)
                     /locus_tag="KPK67_000626"
     CDS             complement(16391..17464)
                     /locus_tag="KPK67_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG2457633.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDKAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(17723..18481)
                     /locus_tag="KPK67_000627"
     CDS             complement(17723..18481)
                     /locus_tag="KPK67_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2457634.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWSSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLFLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLAAVILFNKKPV"
     gene            complement(18478..19395)
                     /locus_tag="KPK67_000628"
     CDS             complement(18478..19395)
                     /locus_tag="KPK67_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2457635.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGERVTYGKTSSNHHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(19395..19595)
                     /locus_tag="KPK67_000629"
     CDS             complement(19395..19595)
                     /locus_tag="KPK67_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBG2457636.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(19600..20580)
                     /locus_tag="KPK67_000630"
                     /pseudo
     CDS             complement(19600..20580)
                     /locus_tag="KPK67_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(20609..22222)
                     /locus_tag="KPK67_000631"
     CDS             complement(20609..22222)
                     /locus_tag="KPK67_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2457637.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAYYGA
                     YAPLGYKKHPDIKGKLLVDEETKWIVEKIFSLAYQGYGSAKITKVLREEKVPTASWLN
                     FTRYGTFAHIFEGKPDSKRYEWTIAHVKAILKSEVYIGNSVHNRQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNTANKTPYSYYACSFYGQFGKGYCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASALKKAENRQNEIDRLFAKMYEDRACEKITERNFIMLS
                     GKYQKEQIELEQQITNLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGKVD"
     gene            complement(22334..22561)
                     /locus_tag="KPK67_000632"
     CDS             complement(22334..22561)
                     /locus_tag="KPK67_000632"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBG2457638.1"
                     /translation="MTKAVELLRKDFYRKGVCVIADIVKTRQEKPNNKCGIGNRGKTK
                     IVVKEHFSEKGKTMEELLTDVMLEKAKQTIA"
     gene            complement(23337..24551)
                     /locus_tag="KPK67_000633"
     CDS             complement(23337..24551)
                     /locus_tag="KPK67_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG2457639.1"
                     /translation="MNDKNFIETLRQKREEYGVTQTRIAVACGISREYYNRIEKGKQP
                     LNDELKEIIEKQIERFNPREPLFLLIDYFRVRFPTTDALKIIRDVLQLKADYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQLESYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDRAGILDIPKLKEKYMAGECVSYFRKQKNYGSTEKCGDDMPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKIGTEVEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWKMNEDWAWFVGDNREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMIQALDRENHTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTVVPQENDGIF"
     gene            complement(24683..25057)
                     /locus_tag="KPK67_000634"
     CDS             complement(24683..25057)
                     /locus_tag="KPK67_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457640.1"
                     /translation="MLILGIIVVIVSLYLLYLKYRVVFTGEKCKGKIIGIVDQNAGYV
                     VGGAFVKKHAYIIKIKNKKYYTAHGCILLSLGKRKIGKEIFVFKNEKYGKEVFKCFDF
                     RIEIVTLLTCLLGVFLIYESLQ"
     gene            complement(25154..25378)
                     /locus_tag="KPK67_000635"
     CDS             complement(25154..25378)
                     /locus_tag="KPK67_000635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457641.1"
                     /translation="MDCEMTDIIAIISSIIAVGYSIYSIVTKGYIRQYNLKKFEHFSL
                     NQKKVCIAFNFIMTLIIGISGVYILLLYFS"
     gene            complement(25742..26272)
                     /locus_tag="KPK67_000636"
     CDS             complement(25742..26272)
                     /locus_tag="KPK67_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457642.1"
                     /translation="MEKILENTLLLMILPWIFVWGILCILEKRKRKNEDGNKDEKRYV
                     IQNDNIFFPLFSLGMFLFSFCLFYTVFFTEFDISEVISVSAFVIFFAILTLDSVIWRV
                     IVDGDIIKYRSFYGITRTYNFNDITKGVYTKKGSLKIYIEGKRIFTFYDSLEFSLFEN
                     QMRKRKIPLEYKRKEK"
     gene            complement(26724..27032)
                     /locus_tag="KPK67_000637"
     CDS             complement(26724..27032)
                     /locus_tag="KPK67_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457643.1"
                     /translation="MKKLYCMNCGAEIKGGIPNECSCGAEFDLADTKNIKKIITSIIF
                     FVILMSPLIILIHFGRQYLQASIILYALLLILGIFGCRIVQTILVRLGLVTMTNIEIK
                     "
     gene            complement(27068..27802)
                     /locus_tag="KPK67_000638"
     CDS             complement(27068..27802)
                     /locus_tag="KPK67_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457644.1"
                     /translation="MSKPKKVIWVILGIIIFVFSVFLGLGYLGQITGGNSLIQRTEMN
                     DKYVPEEITKYYPIENLNSKENLLSDKNYANSIQDALLSASIEFEQGEEYKAHIDKII
                     KEFENENYKSVLYISEKNDIESSLTFSKFKIKEVDGKKRYAYITSVHEVIKKDRPYDK
                     DTMSLLKSQLALSDSLQDLNISPDNSRFLYGFVHDEDIYNTKIENKKPDEIIYFELCE
                     KPFYFWYYENFQSDKSGKSLSIEIER"
     gene            complement(28179..28307)
                     /locus_tag="KPK67_000639"
                     /pseudo
     CDS             complement(28179..28307)
                     /locus_tag="KPK67_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(28817..28957)
                     /locus_tag="KPK67_000640"
     CDS             complement(28817..28957)
                     /locus_tag="KPK67_000640"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBG2457645.1"
                     /translation="MNGEGCILSNKITKECYKIGYMIREKSIDTYPDSGRGFMTWDEN
                     GE"
     gene            complement(29266..30573)
                     /locus_tag="KPK67_000641"
     CDS             complement(29266..30573)
                     /locus_tag="KPK67_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2457646.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDI
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(30638..31117)
                     /locus_tag="KPK67_000642"
     CDS             complement(30638..31117)
                     /locus_tag="KPK67_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBG2457647.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(31164..32027)
                     /locus_tag="KPK67_000643"
     CDS             complement(31164..32027)
                     /locus_tag="KPK67_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2457648.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(32027..32782)
                     /locus_tag="KPK67_000644"
     CDS             complement(32027..32782)
                     /locus_tag="KPK67_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2457649.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     SDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(32809..33297)
                     /locus_tag="KPK67_000645"
     CDS             complement(32809..33297)
                     /locus_tag="KPK67_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2457650.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(33315..33716)
                     /locus_tag="KPK67_000646"
     CDS             complement(33315..33716)
                     /locus_tag="KPK67_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBG2457651.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(33719..34216)
                     /locus_tag="KPK67_000647"
     CDS             complement(33719..34216)
                     /locus_tag="KPK67_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2457652.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(34240..34644)
                     /locus_tag="KPK67_000648"
     CDS             complement(34240..34644)
                     /locus_tag="KPK67_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2457653.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(34874..37441)
                     /locus_tag="KPK67_000649"
     CDS             complement(34874..37441)
                     /locus_tag="KPK67_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2457654.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            37607..38350
                     /locus_tag="KPK67_000650"
     CDS             37607..38350
                     /locus_tag="KPK67_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG2457655.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKDKLLDVDYTNI"
BASE COUNT        12225 a         7245 c         4254 g        14779 t
ORIGIN      
        1 ccttacttag acccagcgaa ctatgcagat tatattacta atgaagaaat tccccaagat
       61 gaattgagaa atgaccctca aatttctaat ggttatacaa aaatacctaa taaccagaac
      121 ataaataacc tatatcctaa taatgtaaat cattatccta aaagtagtaa ttctccaaac
      181 aacttgccta acacaaataa tacatccgga aatatgaatt acaatacatc tgctacaaat
      241 aatatgccta ataacatgaa taataatacg ggtatgccta ataatatgtc taacaatatg
      301 aatagtaata tgggtatgcc taacaatatg cctaataaca tgaatagtaa tatgggtatg
      361 cctaacaata tgcctaataa tatgaatagt aacatgggca tgcctaacaa tgtgcctaat
      421 aacatgaata gtaatatggg tatgcctaac aatatgtcta ataatatgaa tagtaatatg
      481 ggcatgccta acaacatgtc tggtaatatg aataatatgt atggtaatac aggtatgcct
      541 aataacatga acagtaatat ggggtataat atgaataata ctatgggtat tcctaacttc
      601 tcatgtaatc aaaatatgcc tcctaatgta cttatgatga taccaggagt tatatgtcat
      661 aatacaatgc aaggtatgcc tgtagttatg cctagtacta tgcctcctaa tatatatcct
      721 actccttatg gttctagtaa tatgtctata caaggtatac cacaggctac taatattgaa
      781 gaatttgatg aggaagaaat gtaatatata tttttttaag taagttgtat aaaacattaa
      841 taattgacca taataataaa tgtattcctc ataatattcc cccaatatta ttataggtat
      901 agaaagttga ctgaatatgc gcttattcgg tcaacagata ttaacataat ctaataagat
      961 aaacatagaa aaagtcagat aaaatttaat tttatctgac tttttcattt aaaaatgctc
     1021 tcttgtgttg gtgggtcaga atagtatgat actcgtattt gagactaata ttggcaaaca
     1081 acataaagaa aagtataaat tttttataat aaactgtata aaattttaat aattgaccac
     1141 aataataaat gtattcctca taatattccc ccaatattat agaaataaat ttaagcgaga
     1201 ttaaattcag ttaatctcgc ttaaataatg tctaaattta ttattccaaa aaatcatcaa
     1261 aatctaatga acctgattct gcaaaatcat ataatgcatt ttgctcaaag aaaaatttct
     1321 cttcaaattt aatatagtcg ctataattag attgacttat ttcattgata taattgcaat
     1381 cattttcgtt ataagtacca ataaaaaact tatttcccat taagtaatat atagctttca
     1441 tatcattatt tattatatat acattttctt ttacatggtc attattaaaa ttaaatctag
     1501 taaagaatat accttcattc atattacttt ttaacagaaa tggattttct ctataaaata
     1561 ctcctgcaaa ttcttcaggt gaatttacac tgtaaacact tacaaattca cttttagaaa
     1621 tctcatcaaa tacctcaaaa tcatacatag gttctttatc tgtcactaat agagaattaa
     1681 ttctaaaacc agtttcataa cacttactta tgcaaaaagc aattttacat atataatatc
     1741 catcattgtc ttcatataaa gcttcactta tatatctgcc ttgaccttta tcacttacca
     1801 cgtttttaag taatgttcca tttatattag taatgtgcat atttgcaatc tcgtcacttc
     1861 cagaaaagta ctttaaactt tctctatccc atgctataaa tctcatggtc atatagttta
     1921 taaattcaac ttcctcgtca actcttttac atattttatt gtataatttt tctatagtta
     1981 aatctgtttt atagtttttt ataatatcaa tatactcaca tttatctcct ggcaatagct
     2041 tatcataata aagattctta ttaccaaaat aagatactaa aaataaagat tcatccttcg
     2101 ttaattcaac tctatctgaa ccaaatcctc ccatcaatct ttcttcttcc atataagctt
     2161 cttcttgtgt tgggttattt aaactcacat aatctgctaa tcctaatccc tctgcatcta
     2221 ataaaaaata ttgacaaaag tgattgtttt catcatctat ccaatttatt ataagaccta
     2281 aactgcccat aagtctagaa tttgtagcct ttgcaaaatt aaaattcata tagtcacatc
     2341 ctttaaactc taaaatactt gttttttaat atataaatta ttatacatat attttaacaa
     2401 cttagataca atttataata taatataagg aaatgattaa ttgaggtggt cttatgaaaa
     2461 atatagcagc tttttttgat atagatggta ctctttatag agactctctt atggttgaac
     2521 attttaaaaa attaattaaa tacgatataa ttgaccaaaa aaattggtat aaacatgcta
     2581 gagacacttt tatggattgg gataaaagac aaggtaatta tgatgattat ctattagaaa
     2641 tatgtgacct ttatgttgat tcactaaaag gattagataa aacctgtatc aactttacaa
     2701 gtgaccaagt aattaaatta aaagcagata gagtttataa atacacacgt tcaaggattg
     2761 agtggcatct aaatcaagga cacatagtta tatttatatc tggtagccct ggatttcttg
     2821 ttgaaaaaat ggcaaaaaaa tataatgtaa ccgatttttt aggaagtgat tatgtctttg
     2881 aaaataatat atttacaggc acagttattc caatgtggga ttctataagt aagaataatg
     2941 ctataaatga ttttgttgta aagtatgatt tagatttatc aaactcgtat gcctatggag
     3001 atactaatgg tgatataaat atgctaaaaa gagttggaaa tccaatagca ataaatccaa
     3061 caaaggaact tttatctcaa atatcacttg atgaggaaat ttctaaaaat gttgagataa
     3121 tagtagaaag aaaagatatt atttattctc tatctacaga tgtaaatata gtggatattt
     3181 aatttatact atctatttta aaaacttata ttatatctaa tatttatatc taacatataa
     3241 aagctttgac taaaaatcaa agcttttata tgttgcacta agaatatgtt aaatttagct
     3301 ttatgcttta atgtatattc agatatatca tttacactta tctaaactac aatttcatta
     3361 aaattaaata taaaaatcaa ctattatttt ttaaatagca tctttcttaa tctattattg
     3421 taataaccta taaaagaact gtaaacatag tcatatacta taaaggctat ctcaaatgaa
     3481 cctataataa atatgtttat tggaatataa actattgttc ttaataaaaa atacaatata
     3541 agtatcatta tatttgcaaa tactaatttc ataaaatact caatatatat agacctatct
     3601 ttttctataa gatattttac aataccatat attccaaagg taagtgaata tacaatccat
     3661 tgagacttac ttgccatcac tataaatcct aaaactatag aacctatata aaaagcaatt
     3721 ccactcttaa agccccattc cattattaca attgatacta aaagtgatgc taaccccata
     3781 ataaacaatg tatttacagg aataatattt gtaagagtca acaatatcac atttaatgct
     3841 aataaaactc ctccataagc aacttttata ctcatattta tcatcctaac aacaagttat
     3901 taaatctcca ccaaaacatt cacaacatga atctaaacac caaagcttag cacaattttc
     3961 acaacaatcg tcacctagac aacaacagca atcatcacag caacagcaac aacatccatc
     4021 taaatcatta cgtcttcttc tattataatt gtcagcccta ttgctataat catttctata
     4081 ttgtgtataa cttctaagtg catcactata ttcactattt tcaggctcca taaattttgc
     4141 tctttttaag aagtcttcac cttcttcata atatcctatg ttcattgcag aaaagccagt
     4201 taaataatac cactctgcac atttgtcagt caaagttttt aaaaaatcat atgctttttt
     4261 aaattcacta ttttctatga atgctcttgc tctcataaaa ttttcattat tataagtatt
     4321 actgtacatt ctttccacct ctactttcta atatattaat atatcttaaa taaacaccag
     4381 aataaattat attttctatt attccaacat ttttatttag atttagatta tcaattcccc
     4441 tagctaaaaa acctaatgat gttactatta ttttttttac tttttctttt aactcatcat
     4501 ttttatcaat atactctata aatggattat atcttccttt cttaaaatct tcatctaaat
     4561 cttcataggc atctagtaga tatatatact tacctatatt aaaacctatc attcttaagt
     4621 ttctctcatt ttcatctttt ttatagacaa atacttcacc cattatattt ccaaatatac
     4681 tagatacttc atctatactt atatttttat ctttttctaa cttatttaat tcatttagtt
     4741 gttgttttat atactctgct ttttcaggat acttttcata tgctaatttt aatttgtttt
     4801 tatatatata atatgccaat ttatctttta tgcgtttatc atccattaaa ttatcttcca
     4861 atttataata cgttaaaaga atattcatac ttgctgcata ttctgtaata tcatttgtaa
     4921 ttaccttttt ctttttaaat ggatttacta tacacacttc ctctgtactt atagactctg
     4981 ggttatatac tgctgttaat aaaacaatta aaaatgttat atcataattc aaagaaaacc
     5041 tagagatttc tccatgattt ctttttaagt atttacatag tccacaatag taagctttgt
     5101 aataatcata ttctctaaat gttaaatcca ttttgtttat tttaacatat ccaaacataa
     5161 atactccttt ttgtactatt ttttatttag attatatata gtataacact ttttatcaag
     5221 attatataat ttacagtatt ctaagaaact taaaaaattc cttctaatca ttgtatctta
     5281 aatttaaaat aattagaagg ttgggttaaa aataatatta aattatatac ttatattaac
     5341 tcatacaagt tagttgtgct tatatcaatt taagttttaa ataattggta tatatttaaa
     5401 actacctaca gtatccctca attctttttt tgctttattt ataaccaatt catcaagcat
     5461 tacatcattt attgaacatg atatagattt agatgccaat aacattgcct taaatcccat
     5521 atcagaacct gcactggcac aagattgcca tgtatgggca gaaataccaa taggccaaac
     5581 tgaacaagaa cactgcgctg taggaattat gtaactaaca tctccaatat ctaatgataa
     5641 tgatatacag ttattatgag taaatgtatc atccgtaact tcatcatgca gattcatctt
     5701 agtaatatcc ttatttccac ttaatacact agaaacacta cttcttttat ctatagacac
     5761 tgtctcagct aatttatctg caaaatagta gtcatcttca ttatagtttt gcacacctac
     5821 taatttcata ttttgtgata gaatatctgt taatattgta tttggaatat agtcatatac
     5881 tccacttata atgtcatatt ccataatagt accagtcatt aatgctgctc cttttgctat
     5941 atcaacaatc ctaccaaata catctcttac atgctccatt ttaactcctc ttatgtaata
     6001 ccacacttct gcaaaatcag gaactatatt tggcattttt ccaccattag tagttacata
     6061 gtgtactcta caattatcaa atatatgttc tctcaaataa ttacaaccta tattcataag
     6121 ttctactgca tcaagtgcac ttcttccgtt ataaggagct tgtgctgcat gagcactaat
     6181 cccattatat ctaaatttca cagagtaatt agcaagtgtt gggattctta tcggcgtatt
     6241 tatatcaaat ggatgccaac taaaagcaca atcaattcca tcaaaaaaac ctttttttat
     6301 catcaatact tttccagata aatcttcttc tgctggacat ccatagtatt ttatagtccc
     6361 tttcagcttt tttagtttga ttaattcctt tactgcaatt acagctccta ttgaacctgt
     6421 accaagtaag ttatgtccac atccatgacc tgcaccacca tctacaatag gttctttagt
     6481 agtggatact ttttgagaca aaccatctaa agcatcatat tctcccaaaa ctgctataac
     6541 tggtctacct tctccataac tagccacaaa tgctgtatcc ataccttcta atccctctat
     6601 actaaaacca tgttttttta aacattcttt ttgtaaattt gaagcatagt attctttata
     6661 ctgaagttct ggatttttcc atatcttatc tgatatatct cttaattctc cctgtatctc
     6721 atctatatat tccatcaaaa actttttatt taaattatcc atattaatta tataaacctc
     6781 cctgtttcta ataacttata gattatcagc caaaatgact aattttaact gatttaaaaa
     6841 tattttctac catacaacca ttttatacaa taaaacctct tgacttgtaa gtttcatttt
     6901 atcaagtaag aggttttcaa gttttataaa atttttagca tgttaaccaa tcaagcatat
     6961 taacccataa tttattatag tatttccaat cagtaaattc ttttggaccc cagtgaggtg
     7021 cacagtcaga actaaagata gcagattttc cttttccaaa ctcgccaaca gctacaaatg
     7081 ggtctcctcc aatagttgct aaaacaggac aattatctct agctactgtt ttgttatatc
     7141 ctaaaaactt tggccattct gttggtatac cttttaatac tggatgttca gagtcaataa
     7201 cttctggaat tattcctgct ggtttttcaa ctctatcatc tttatctatc attgttattg
     7261 gtaatacatc tttgatagca gtttctccga atctagcttt tgcatctatt cctgtaaatg
     7321 acatatatcc acctaccatg attaacgccc caccattatt tacatactct ttgattagtt
     7381 caagtctatc tggattacta ttgcaatcta taaaagtact attagataat aagaacgtat
     7441 tagaacctat atcagataat attatacagt catactcatt taactcttct aatttatacg
     7501 gaaaactatc tgctgctaca tgtgcaggca tatgagctgt ctcatatcct ccagattcta
     7561 ttgcttcttt caaccacttt acagcttctt cataatgagt tgtaacaaac gtatcaaatc
     7621 cttttatatg agttatattt tttacccaag attcccctac tattaatact tttttcattt
     7681 atatattcct cctactttta taagtttaat attagacttg catacataag tatactatct
     7741 aaatactcct taatttcaac atattcatca attgaatgac attgttctat tgaacctggc
     7801 cctaatataa gtgttggtat ttcagctaaa tttttcatta atctcgcatc attgccagct
     7861 gttgaacctt ttatctctaa ttttttccct agagtttctt ctagtatttt atgtgcattc
     7921 actacaaaat cagaatcctt gtccatctca aaacctaacc ctgattgata tatgtttata
     7981 tttggtttat tatctttaag ccataaatct ccattagccc ttgtcattat gacatttgtt
     8041 acatcattta caacttgttc ataactcatt atatttggca agaagtgtac acataaatta
     8101 aatacacact tatctggcac agtagaacct gctgttccac catttattac acctaaattt
     8161 attgtaggta aaggtaatag tggatgctta tatatcataa gccagttatg ttccaactct
     8221 tttatatctt gaagcaacaa catagccttc tcaattgcat ttacaccctc ccacttacta
     8281 ccacagtgaa gagacacacc tttaacctca acttcaaaga atacaaatcc catatgtgca
     8341 actattaagt tttgttctga tggttcacaa attatgcaac aatctccacc agtaccattc
     8401 ataactgcat ttatggtacc atttccgcca ccttcttcat ctactactga cattattttt
     8461 acattaccag gtacatttag accactatct tttataagct ttacagctaa tattgatgct
     8521 ataagtccag atttcatatc agcagttcca agaccataaa gttttccatt atcttctgta
     8581 gcacgatatg gattatattt ccacttactt atatcacctg gtggcatagt gtctacatga
     8641 ccattaaata caattgtctt accaggtaaa tcatcactat acttacaaat taaattatat
     8701 ctatcttgat aattgtgacc tagatttcct tctttatata tattttttgc ttttttaatt
     8761 aattcttcgc tcatttcttg tctatctaca ctaaagccta tgtaatttgc aagtttttca
     8821 atatattcct gtccttcttt ttcaaatcca cctagtattc catgaccaac atcttcagtc
     8881 tttatactta caagttcttt taaataatct atgtattctt gttttttact atttaatgta
     8941 tctaagagaa tttttttcat atttcctcct aagatttatt tttacattta aaatttcaag
     9001 tctaatctca tctatttaag ccatagtaaa ctttttcact tagcatagtc ccattcaatg
     9061 tataaagata tatgcaaatt aaagtataaa aatatataca aaaacttaat aatttttata
     9121 gtttataaac taatatttaa ttactattta gatttagctg atttttttaa ttttatatta
     9181 tttatccata tagcaaataa cattaccaaa ccttttataa tcatttgatt atatgaagat
     9241 acacttaata agtttaatcc atttcctaat attcctatta ttccgacacc tatacatgta
     9301 cctactatag aaccagaacc acccatcata ttagtaccac caagcacaac agctgctata
     9361 gcatccaatt ctgctccatc tccgacagtt ggtgaacctg atgccaaacg tgatgatagt
     9421 attatcccag ctaatgctgt tagtagtcca gctaatgcgt atacttttac tataacttta
     9481 tttactttta ctccacataa tctagcggcc tcttcatttc cacctactgc atgtacatga
     9541 cgtccaaaat tagttctttt taatagaaaa tgtcctgcta ttacaactat aatcataatt
     9601 ataactggta ttggtattgg tccaatatat cctctaccga gcatcttaaa tgcatctgta
     9661 tttactgata taggtcttcc tcctgtaaat acaaagcttg ccccacttat tatcgttaac
     9721 atggctaaag ttacaatgaa tggaggtagt ttttgtgttg aaataattgc accttttatg
     9781 actcctaata ttgtccctat tgctaatgca agtatcatac ctacccatat aggtgcatta
     9841 taattcacta ctgttgtagc ccaaaatgta cctactaaag caattacttc tcctacactt
     9901 aaatctattc ctcctgtaat tattacaaat gtcataccta ctgcaagtat tgcatttatt
     9961 gatatttgtc ttccaacatt aattaaatta tctaaagtta aaaagttaga tgaagctaca
    10021 ctcattatta taaaaattat tacaagtcct acagctgcac tcccttggtc tctaaataca
    10081 cttgttaatt ttgtcaataa tggagaagat ttttcttctt tatgcttagt tattgaactc
    10141 attaatttta cctccgaaag ccaattttaa tacattattt tcaaccattt cttcaccttc
    10201 taaaaatcca cttatatgac cttctctcat aaccattatt ctgtcactca ttgatattaa
    10261 ttctggtatt tcagaagata tcattattat acttacacca ctctccacta aatctcccat
    10321 tagattgtat atctctgcct tagcatttac atctatacct ctggttggtt catccataat
    10381 caatatatct ggttgtaata aaagccatct tgctattata gctttttgtt ggtttccacc
    10441 acttaaactt tgtataactt gattcatatg tggtgttttt atctttaaag cttctatgta
    10501 tttttctaaa atctcttttt ccttttttct atctataatt gatttaaact tagatatctt
    10561 ttttaaccca gccatagttg tgttttcttt gacactcatt tgtaaaaaca atccagtacc
    10621 ttttctatct tctgttatta atcctagtcc attttctatt gcatctgatg gagttttaaa
    10681 ctttacttct tcattgttta aatatatttt cccagaatca tatgttctaa gtccaaatat
    10741 agcttctact atttcacttc tcttacttcc aactagtcca cctattccca atatttcacc
    10801 ttttcttata ttaaagctaa tatcattaaa aacattgttc ttagttagat tttccactcg
    10861 cataatttct tcttttattt ctacttttct tttggggaaa aattggtcta gctttctatc
    10921 tatcattaaa ttaactaatt cttcctcaga cgatacatct ttggtattca aagtcgaaat
    10981 cgttttccca tctcgcatta tagttattct gtcacaaatc tcaaagagtt cttccatcct
    11041 atgggatata tatattatac taactccact ctcttttaaa tcatttataa gtctaaaaag
    11101 tatttcagtc tcttttttac ttattgaaga agtaggttca tccattatta atactttagt
    11161 ttctgatgat aaaactcttg ctatttctac catttgtttt tgagctatac taagctcttt
    11221 gactaaaata tttggattta tatctattcc catattatcg aaaatctttt tagcctcttc
    11281 attcattttt ttgtcattag ttaagacttt gccatttatt tcttttccca taaaaatatt
    11341 ttgtgctact gttaattcgt caaaaactgt tagttcttga tatatggcat ttatacctat
    11401 ctttttagag atatctgggg atatattctc tatttctttg ccttcaaaaa taaattttcc
    11461 agaagttttc gaatgtgcac cagttaaaat ctttatcatt gttgatttac cagcaccatt
    11521 ttcacccagt agtgcatgaa cttcgccatg aaataactca aaattaacat tatctaatgc
    11581 tctgacacca ggaaattctt tagcaatatt acttaacttc agaattatat tactcataat
    11641 gacactcctt tttaaatagg atttctttca aataatctca ttttatttta ctaattctta
    11701 aagtctttta tattttcctt atcataaagt aaagctggta ctggtatatt cttttctacc
    11761 ttttcccctt ttatagcttt tactactgct tctacagcta aacgccccat ctctttaggt
    11821 tgttgttgaa tcatagcaag aatagttcca tctgttattc cacttgatgc ttcttcagtt
    11881 aagtcaaatc ctacaatctt aacatcctta ttttttgcag attcaagtgc agctttagct
    11941 cctagtacac tattttcagc tgttgcatat attatatcta catctggatt agattgaatt
    12001 aagttttcta ctgtattaag agattcttct ctgtcatatc ctggttgtga atttaatatc
    12061 ttaacatttg ctgaaccttt taatgcatct ttaaatccat ctattctctg catttgtatt
    12121 tgagatttta aatctgtcac tattgctatt tctgactttc cacctaactt ttcatctatg
    12181 tattttttag tgtattcccc taatttttta ccagcatcat aactaactgt tcctacataa
    12241 cttgtaatat tttcagtatt taatacagca tctactgtaa ctactggcat tttttcaaat
    12301 gctccttcta ctgctggtac tatagccgaa ttatcagttc caagtactat cattccatct
    12361 acattctgtt gtataaaatc ttctattgct gatgtttgct tagctacatc ttggtttggg
    12421 tcatttacta taactttcac acctaattct ttagcttttt cttttattcc ctcatcaata
    12481 tctataaaaa attgatgttg aagagttatt gtagatactc caatagttaa atctttcttg
    12541 tctgaaccac ctttattatc tgatttactt ggtccttcca ttgaacaccc tgttaacatt
    12601 cctattgcta gtattccagc aattcctaaa gataaaattt ttctcactct cataattaac
    12661 ctccattttt aatagtttta aatttgaaca aattagattt ttatttatta tttaaatatt
    12721 tttcaacatc ttctttatag ggcataccag attgtgcacc tagtttagtt acagaaagac
    12781 ctgatactac tactgcatat tctactgctg tatataaatt tttcccttct gttatagcta
    12841 ctgccaatgc tccattaaat gtatctcctg ctcctgtggt atcttgaaca tcaacactta
    12901 tacaagatac atgcttgatt tcctcaccat cataaaatct agctccattt tttccttctg
    12961 ttattacaag tttatttgga tattttttta atacttcttc tatcccttca tttgtgtcaa
    13021 aaactatctt atattcatgt tcatttggtg ttaagtaagt tactttttct attatatctt
    13081 catttagttt tactgctggt gctggattta ataaaacctt aatcctattt tcaaaacaga
    13141 aattcactac ataatttatt gtttttaatg gaatctctaa ttgtagcaat actatatctg
    13201 cattctttat ctcttcttca ttctttttaa ttaattctat atctactagc tcattcgtcc
    13261 ctggtacaac aacaatacta ttatcatttt cacataatgt tataacagca acaccagaac
    13321 ttttgtgatt atttctggat actaaactat aatcaacttt attggttcta aaatttctta
    13381 ttaactcctc tcctaagcca tcttccccta tgcatgatat catttttaca tttgctccta
    13441 gtttagaagc tgagatagct tgattagccc cttttcctcc aggtgatgta aaaaaactat
    13501 tacctaatac tgtttctcct ttttctggtc ttttatctac agaacatacc atgtccatgt
    13561 ttacacttcc tatgacaact atgtttccca ttacattact cctttacata tctatttatg
    13621 tctctcgtgc tgttattcac tttcttttgt tgaatctctt atgaccaatt ctggtttgaa
    13681 aatcaattca tcaaacgatt cttttttatc tcctcttaaa caatttatca atatttcact
    13741 ggctttatat cctatatcat aagacggctg ttttactgtg gtaagagaag ggcttattag
    13801 acttgatgtt tgaatatcat caaatcccac gacacttaca tcatttggta tacttaatcc
    13861 tctttcttta agacctctta tgacacctat tgcaatcaaa tcatttgcac aaaatattgc
    13921 atcaaaatct atcttttcta aattttttat aaattcttgt ccccattcaa tcttatattt
    13981 tccttcaaat acataagcag gattgtagct taaattacac tcgattagag cctttttata
    14041 tccttctaat ctatctactg ctatttcatt ttgaagtgga ccacttaggt atattatttt
    14101 cttatttcca atatctaata aatgttttgt agctatatag gccccttcaa agttatttat
    14161 tttaacaatt ccttttaagg tttctttgtt catacttatc ttcttatcaa tcaagacagt
    14221 tggtattttt atatctttat aatttgcttc tttccagttt gtatttgaag atgctgcaaa
    14281 tataattcca tctatcatcc tctctgatag tgttcttatt ccttcatatt cttttttagc
    14341 atcttcatct gtattacata aaattatatt gtaaccttct tcattagctt tgtcttctac
    14401 acctcttgat acatctgtaa agaatggatt tcgtatatct ggtataagaa gccctatagt
    14461 attagttttt ttagttacta aactttgtgc taatttattt ggtacataat ttttttcctt
    14521 cattattttc aaaatttttt ctcttgttgc ctgacttata ttttcatctt tattattaat
    14581 gacttttgac acagttgttt tagaaacacc agccaattcg ccaatttctt ttatagttat
    14641 actcaagaag ttcaccccca aaatttagga aaccgtttta cttaaccggt tacctagata
    14701 ctatcacatc ttttttccat ttgcaaccat tttatataaa ttttgatatt tttccctttt
    14761 ttcaaaatat tgcaacttat aattcaattt taatatttaa attaatctgc aaaaaaaaat
    14821 taatatactt ttataattat ctaaaagtat attaattaat ttaagcagta tttattaaag
    14881 ttcaaatcct tgctctataa tcattttttt atcattttca atcccacttc caacagttgt
    14941 caaataatca ccaacaattg cagaatttac tcccccatta aatccaactt tgtcatatcc
    15001 ctttaatgat agccttccac cagcatacct tatgtacaca ttaggaatta taaacctaaa
    15061 tactgctgtt gtttttagta tttccattgg ttctaataca tcataatttt ccattggtgt
    15121 attcttaata ggatttaaaa tgtttattgg aaatgatttt acatttaatt ctcgtatttc
    15181 aaaagccatt tttagtctct gttcttcact ctcattcatt ccaattatac cgcctacaca
    15241 tacatctagc ccagcttttt tagcatcttt tatagttttt atcctatctt tatatgtgtg
    15301 tgttgtacat atattatgaa aattatctct acacgtctca acattgtggt gatatgtagt
    15361 cacacctgct gacttcaaca ttttagcttg ctcataatct ataataccta aagatgcaca
    15421 taattttaaa ttagtatttt ccctcaatcc ttcatagata tttaaaatat ttttaaactc
    15481 ctttcctgac atacctttac cacttgtaac caatgagaat ctatgcactc ctttactctc
    15541 catctcttta gctctattta agatttcatt gtaatctaat aatgaatatt cttctacacc
    15601 agttttataa taagaagact gagcacaaaa tttacagtct tcactacatt tgccagattt
    15661 agcattcatt atcgtacata aatctgcttt tcttcccata aaatattctc ttatttcatt
    15721 tgcagatttt aatagaatat caaaatcgtt tttattattt gtatcaaggc ttattaaatt
    15781 aagagcttct tcataactta tttctttttc tctcaatact ttatttttta attttattat
    15841 gtatttttcc atatatcctc ctatttgtca acttaatatt tttatgtagt ttacttatta
    15901 tatatagtat tgcatatata acatttattg gtcaacttta ttttagacac aagtttacta
    15961 tacacaaaga aatactgctt caaaataata ttagtatata gattatacat attattatta
    16021 aagcagtatt ttttattaga atataattct tactggttta taaaactatc cttgtcttaa
    16081 ctccatccaa gctttccatt gtaataacct ctaagccttc atattctcta aatagccatt
    16141 ttaaatcctt catggcttga ttaacctgac tttttgttag attatttata aaatattctt
    16201 ttctagtttc ctcatactct ttgctagtca aaaagtttct tagaaaatta aacgcaaata
    16261 cacttggaat attgtttaca ttagccttct tgctgttttc cttctcatag taaattttca
    16321 taaatgcata ctcctttgat acttttagat ttctcttaca ataattatat gcatttaagt
    16381 gctactattc ttataatttt attaattcat attctttact tcccataccc atttcacatg
    16441 catagtctat tattctagtt ccatttacat gaggatgttc atgtttgaaa acatcatgac
    16501 ccatttcctt acaaatcaaa tcatagcatg cttgttcaat agctacaggg tctgttgatg
    16561 ctaatattcc tatatcatgg gcaattggtc tacctgacca aggtacacaa tcacaaagtg
    16621 gagttacatt cataacaaaa gttatatacc ctaccttgtc ttttttattt gatactgctc
    16681 cataagcgta ttctgccatt ttttcgacaa acacatctga gtcactttcc cattgtatag
    16741 ttactgctct agttggacag acagttggac attctccaca tccataacat acatcagaat
    16801 ctataactgc ttttttatcc actatactta tagcttttgt agggcaagaa ttaacacact
    16861 ttccacaacc tacacacttc ttttccttta ctactggagt aacatcagaa tgttgcattt
    16921 gttttcctgc tgcacttgca catcccatag ctaagttttt taatgctcct ccaaaacctg
    16981 ctgcttcatg acctttaaaa tgactcatta ctatcattgc agaagaatta taaatttctc
    17041 cacctatttt tacactttca aaatgttttt tgtctatttt tacattctca taatttctac
    17101 tatataatcc atctgcaatt attactggtg catttacaac tgcataagca aatccatttt
    17161 ctatagcagt tgtaagatgg tctacagaat ttgttctact ccctgtatac agtgtatttg
    17221 tatctgttaa aaatggcttt ccttcgcagt ctttaacctt atcaaccact tgtctaactg
    17281 ccacaggact catatatgta gtatttccct tttctccaaa gtgaagttta attgcaactt
    17341 ggtcattctt ttctattaaa tctttgaatc ctgctttatc aaataatctt cttacattgt
    17401 ttggtacgtt tttattttgc gaatttgagt ataaatcaca aaaatatact tttgactttt
    17461 ccatatctac acccctttaa aatatttact tatactaatt ttatcacttt taaagtaatt
    17521 tttagtatat tttaccataa ttaaatattc ttatatttat ttgatacaaa taatattatt
    17581 tgtattattt tctacactta attctatata ttaaactaca cctaaaaatc aaaaagactt
    17641 gaaacttaag tccaagtctt tttgattttt aggtgtagtt tttaacaaca taagattact
    17701 ttaccttgtg ataaaggtat tattaaactg gttttttatt aaataaaata acagctgcta
    17761 gtaaacatac tatagaaata agaactgtta taataattgg aataataaat tctggcattg
    17821 ccactttccc tgaaatcaag tctatatttt ttgacatcaa gaaattagga gtataaggtg
    17881 caatctgttt aggaatagaa attaaactca agaaaagagt tgccaccaat acaagtaaaa
    17941 cactggcaaa tgctggtcta aatagtacac acccaagaat taatacacta atatacataa
    18001 ttcctataag ccataagtta aatgctgaaa atataatatg attaagattt gcagttggcc
    18061 aaaaatacat tgtatagcca taagcaaata aaaaactcga ccaaaaacca attgtcataa
    18121 taatcaccgt aacagagaat ttagacaata ctatagatga acgtgataaa cctttagtca
    18181 gcataataac aagtgtccct ttagaatatt catttgataa aatattacaa aaaataatta
    18241 gagttaaact cataccaaga ccagagatat tgctaaaaaa ttgtgtccaa gaatctaaag
    18301 cagttggtgc agcagtaact tttaaagtcg gtgcaaaatg agcaatcaag tctggcataa
    18361 attttgcaga aataggacta cttataccaa atattagaaa taaaataatt aaactaaata
    18421 atttataatt tcttaaattc tctttaaatt ctttttttgt aaatgctatg tattctctca
    18481 ctttactacc tccaaaaata aattttcaag tgataattct attagttcca tctttactgg
    18541 acaaagattc atttctgcga gtgtagatat aactgttttt tgtattgctt ttatatcttt
    18601 accatgcaaa acaatttcca tattagtttc ttctgaatct tttagtaagg atttaatgcc
    18661 atcactagac ttaaactttt gaatttcatc attacttgca aactcaagta aaagtctatc
    18721 ctttccgtgc atacttttta tttctgaaag tgtaccgcta agagcaatgc ttcctttgtt
    18781 taatatagca acatggtcac agattctttc aacatctgat agtatatgtg ttgaaaatat
    18841 tacagttgtt gagtctttga ttttaagcat aatatctaat atttcttttc gaccaacagg
    18901 gtcaagtgca cttgttggtt catcacaaat aagtaatttg ggacgtgaaa gcaaagcttg
    18961 agctatacca agtcgttgct tcattccacg agaaaaccca cctattcgtc tcttttcatt
    19021 tccaagaccc acaagtgata acaactcttc actccttatc tgtatttctt tttttgaaag
    19081 tcccgtaatt tcaccacaaa gagaaagata ttcaagtggt ctcatataat tgtaaaactc
    19141 tggaacatca ggcagataac ctacatgatg attagaactt gttttcccat aggtcactct
    19201 ctcaccacaa acatctatag ttccactatc tggtttaagt aacccaagta ccattttcat
    19261 tgttgtagtc ttacctgcac cattcttacc tacaaaccca aagactgacc cttctggtac
    19321 agtaaaattt aaattatcta ttatctttcg attaccaaaa ccttttgata atccatttat
    19381 tgagacaata ttcatcattc ttcacctctt cctattgtaa aatacaaaat tggtcctatt
    19441 agactaataa acagtactac aataatccat attgccttat ttccaatttt ataatttgga
    19501 tgtctcaata catgaacaag tgcagtaatt ataagtatta aatctaaaat aataacagga
    19561 accaaaagtg gtaagtattc catcaaattt tccataatct tattcctcca ataaagataa
    19621 tttttctata agaaatttgt attctttgtt ttcacttaat acagaaaaca ttggattatt
    19681 tttaatagca gcaacaatac ctgcttttac gattgtatca tcacgtgtaa tacctgttcc
    19741 aatttcagaa atccatctgt caagtttatt aaaaaagtca tccccatgta ttataattgg
    19801 gtatatatca cgtgttgcta aatctacata ttttcgtaat gatttaatag ctccttcatt
    19861 atcaccatgt attaaattaa tttgtgcctg tgttaaatat gaagaaagca taatagcagg
    19921 agataatgta tcaagctcaa atatatctga aattgctaga atacgtcttt ctatttcctt
    19981 catttgtaat ttatctgcat tttgcaaaat acccactaga ttttggacta atgatataag
    20041 gttctgatat gcaccaagtt gaaatgtttc tctcgcttca tccatttgac cattcatcat
    20101 ttgcccctgt gcaagaagta tagattcatt tatcatgggg aaattgctgt tttgcaataa
    20161 atcaatcact tgtatgggtt gatttaacaa tatataacaa gttgcctcca tactttttgc
    20221 ttgacggcaa atatctatgt cattacaagt ttcctgtata cgaataaata tttctaatgc
    20281 ttcactaatt aatagttcac gctttttttc atctacaata tcatagtgat taactattaa
    20341 aagacccatc cgaaaaatca aaggaaaaca tgaataatat ttttttatga tttctcttat
    20401 ttcatccatg acttcatcaa acggtttaac cgtaaagtct ttgcacagtt tattatacag
    20461 tttattaata tcttccttca tcatttgagg ttcataacat attaactcat caacggtaat
    20521 gttgaaatat gtcgcaagtt gcggtaaaaa ggttatgtca ggatagcttt gtccgctttc
    20581 ccttagttaa agatatgaac cctctttatc agtctacttt tccaataaat cggtagtata
    20641 tctctatttc ctgtattctt tcattgtcct cattcgttgt tgcttcgtga atgaggattt
    20701 tttctatcat tgcatttaat aatggtgctg tcagttcctt tggtacggaa tactccttga
    20761 ttaactcaat ccacttttca gcacctatca tatcctgttc cattttactt aattcttctc
    20821 ttaggttggt tatctgctgt tccagttcta tctgctcttt ttgatatttc cccgacagca
    20881 tgatgaagtt tcgttccgtt atcttctcac aggctctatc ttcatacatt ttcgcaaaca
    20941 agcggtcaat ctcattttgg cggttctcgg ctttcttcaa ggcacttgcc tttttcttct
    21001 tttcccgtat tctctctgca ttgccagcct tttgtatctt attcaacact ttttcttcgt
    21061 cctgctgtac tgccttagcc caatactgca atcgctccaa tacggcttga tacagcacat
    21121 catagcggat gtagtgcata gaacaataac ctttaccaaa ctgtccgtag aaactgcaag
    21181 cataataact gtaaggcgtt ttatttgccg tattcgttcc aaaccgcata gaccagccac
    21241 aatccgcaca cttgacaagc cctgcaaata tcggcgttgc tttttccttt gtctgtctac
    21301 gccttgattt tatctgttcc tgcacacgat aaaacacttc cttatcaata atcggctcat
    21361 gggtattttc tactcgaaac cattcacttt caggcttacg cactttcttc ttgctcttga
    21421 atgaaactgt tgattgtcga ttatgaacgc tgtttccgat atagacttca ctttttaaga
    21481 ttgccttgac atgagcaatc gtccactcat aacgcttgct gtcgggcttt ccctcaaaga
    21541 tatgtgcaaa agtaccgtac cttgtaaaat tcagccaaga cgctgtcgga actttttctt
    21601 ctcgcagtac ctttgtgatt ttggcactac cgtaaccttg ataggctagg gagaagattt
    21661 tttcaacaat ccattttgtt tcctcatcaa ccagcagttt tcctttgatg tcggggtgtt
    21721 tcttatatcc taacggagca taagccccat aatatgcacc ctctgcaaat tttgtcttaa
    21781 atgcagattt cactttacgg cttgtatctc ttgccaccca ttcattgatg atattcttaa
    21841 atggtgcaat ctcactttcg cctttttcgc tgtctacacc gtcgtcaatc gctatgtaac
    21901 ggacattatg gctgggaaaa tacaattctg tatattgtcc tgtcataata taatttctgc
    21961 caagtcggga aaggtctttc gttacaacac agttgatttt tcctgcctct atatcttcaa
    22021 tcattctctg gaaactcggt ctttcaaaat ttgttcccga ccagccgtca tcaatatatt
    22081 catcaatcac attcaaatga tgttcttttg catatagacg taacatactt ctttgtgtgg
    22141 taatgctgga actttcgccc tgtaattcat catctcggct caacctaaga taaagtgcag
    22201 tattgtaaat ctgttgtttc attgtcagtt atcctccttt ataactaacc cgtgtttaca
    22261 atacctgctt gcaagtaaag tataacacgg gtactttttg ccattcaatc tatttcttac
    22321 aactgcaccg attttatgcg attgtctgct ttgctttttc cagcataaca tccgtcagaa
    22381 gttcttccat tgtctttccc ttttcggaaa aatgttcttt tacaacaatc tttgtttttc
    22441 ctctgttgcc aatgccacac ttattatttg gcttttcctg ccttgttttt acaatatcag
    22501 caataacaca tacccccttt ctgtaaaaat cttttctaag caattcaacg gcttttgtca
    22561 tagcccacag gagttccacc tctgcatagt tcttatgtag ccgtacccat tgcgtgcgac
    22621 gctctgaacg ctcaagctgt ggctgtacgg gagtatcatt atcagacagg acaggtcatg
    22681 gcggtaggaa aggacaagtt tcttacagga acacagataa ggttcgctcg ctttagcagg
    22741 gaaaaggtca tggcgtatct gtttcaacgg ctcgctgtct gtcaaacgta ttgaactgct
    22801 ttattcagtt gtcaaagaac acgaaagaag aaaatcgtct ttcctatata ccgcgttgga
    22861 aaagagcaga aacgtaacca atcttcaaaa cttttttctt ttcctgtacc atatagggaa
    22921 agaaagtgtc attctgctaa gttgatgatg aaaaaatctg attttctttt cacaccctaa
    22981 ctacccatgc aacgccgtaa ctgccaactg tggaataaaa gattttcttt gctccatata
    23041 gggaacggaa actgccaaat gttaagttga aaatgaaaag attttcttct tttcacacca
    23101 atactcaact gttactatct gtttgaatac aaattgatga tagtttttct cttttcatag
    23161 aacactgtcc actcaactcg attttccgac attttttcaa aaatttttct tctttcactc
    23221 catataggga atggaaacct cgatttaatg agtaattttg aaaaaaagtt agattttctt
    23281 ttctcgcacc atatctgtac gcaagtagcc gttttgttgc agattttttg aagaaattaa
    23341 aaaataccgt cattctcttg tggaacaacg gtatctatac gttcttctat ggttgatttt
    23401 tctaactgta ataagtgttt atgcttgtct ttgagttctg cctgctcaat catatctttc
    23461 agtatcgttg tatgattttc tctgtctaat gcctgtatca tttttatggt cggtgcaacc
    23521 tgtctttgta accaatgcaa tgccttttgc aaggtgtaag gctctggctt tgtagttaag
    23581 cgtatcggct ctctgttatc ccctacaaac cacgcccaat cctcattcat tttccattga
    23641 cttttcggtt tatcgtcctc tctatctaca aaacggacat aatgattgat gatagaaaaa
    23701 gcagtacgtt cagcgtcccg ataagtcagt aggtctacaa cggcataata ggctcgttca
    23761 tttttcatgc gaatttcaaa gcggttctta atctcggtat cttcaacttc tgtgccaatt
    23821 ttgacatact gctcatagtt cttttgataa gcacacatat ataattcact acttgttgaa
    23881 ccaagatata aagtttcgcc tgtattcttt ggcatatcat caccacattt ttctgtactg
    23941 ccataatttt tctgtttacg gaaataggaa acacattccc cagccatata tttttccttt
    24001 aactttggaa tatctaatat tcctgctcgg tcattgatag ccaaatcaag ccgtttcatc
    24061 acaccgccag ccgtcatgca atcaagcata aaatcatacc atgagcgttc ctgtgccaac
    24121 agataacttt ccagttgccg acagcctttg cctttcagtt ccaatagaac tcctaaatgt
    24181 tcctgcatgg aacacatgat attgatgtcg ccaagcacat atttgctttc atatccgtat
    24241 tttccataat cttcatacag catataatcg gcttttaatt gcaatacatc acggataatc
    24301 tttaatgcgt ccgtagtagg aaagcggaca cgaaagtaat caatcagcag gaataacggc
    24361 tctcgtggat tgaaacgctc aatctgtttt tctatgattt cctttaattc atcattcaat
    24421 ggctgtttgc ctttttcaat gcggttgtaa tactcacggc tgataccaca ggcaacagca
    24481 attcttgttt gtgttactcc gtattcctca cgtttttgtc ttaatgtttc aataaagttt
    24541 ttatcattca ttctaaaatt cctccaatca aaaaaggcaa ctaccgtatc ttgataattg
    24601 cctgttaata aaaatctgta tagaaaaagc agtcaatcct aagaccaact gcttatgagt
    24661 atgaatatga aattatttac ctttattgta agctctcata aatcagaaat acccccaata
    24721 aacacgtcaa aagagttacg atttctatac gaaaatcaaa acacttgaaa acctcttttc
    24781 catacttttc attcttaaat acaaatattt ctttcccgat ttttcttttt cccagtgata
    24841 acaggataca accgtgtgcc gtataatatt ttttattctt aatctttata atgtaggcgt
    24901 gtttttttac aaaagctcca ccaacaacat acccagcgtt ttggtctaca atacctatta
    24961 tttttccttt gcatttttcg ccagtaaaaa caaccctata ttttagatac aacaggtata
    25021 agctgacaat aacaacaata attcctaaaa ttaacattca aattttcctt tcatgcaatc
    25081 tctatatttt ttcatatcag aacaacaata tttaagccag tgatttttaa ttacaataat
    25141 gttttcaatc ccattaagaa aagtataata gcaatatata aactccactt attccaatta
    25201 taagtgtcat aataaaattg aaagcaatac aaactttctt ctgatttaaa gaaaaatgct
    25261 caaatttttt aaggttgtat tgacgaatat aacctttcgt aactatggaa taaattgaat
    25321 acccaacggc aatgatactt gaaataatag caataatatc agtcatttca caatccatat
    25381 ctttcctcca taaaagctta cacattgtta ccacaaaaat taaattattg taatatttaa
    25441 ttagatagtg ttttcctttg tgaaataggt aatttttata tcacacctta taaaatgcac
    25501 ttttaccttg taaatacaac gttttccgtt tgtaaatgtc tcttgtctct tgttttttct
    25561 gccccccttg ttagataacg ggggcttaat cttcgctcgc cacggctcgc tcacagacga
    25621 agtgaaccag ccacttgcaa gacatggcag aaaaatctga gagattttcc tgccaccgtg
    25681 tctggatcac tccgtcttta aagtggtaat attattaaac taattctaaa aaatttcctt
    25741 attatttttc tttgcgttta tattctaaag gaatttttct ttttctcatt tgattttcaa
    25801 acaaagaaaa ttctaaacta tcataaaatg taaaaattct tttcccttca atatatattt
    25861 ttaaagaacc tttcttagta taaactcctt tggtaatatc attaaaatta tatgtcctag
    25921 taatcccata aaatgaacgg tatttgatta tatcaccatc tacaattact ctccagatta
    25981 cactatctaa tgttaaaata gcaaaaaata tgacaaaggc agaaactgat attacttctg
    26041 atatatcaaa ttcagtaaaa aacaccgtat aaaaaaggca aaatgaaaat aaaaacatac
    26101 ctaacgaaaa aaggggaaag aatatattat cattttgtat aacatatctt ttttcatctt
    26161 tatttccgtc ttcatttttc cgttttctct tttctaagat acatagtatt ccccagacaa
    26221 atatccatgg cagtatcatg agtaataatg tgttttctaa tatcttttcc atatatctct
    26281 ccacgttttc caaaattgat aaatatatta tagcacttac ctcatttcag ttcattactt
    26341 ttctgctatc tgtcagaata ttttttatcc catgccttat caagagtgca agcattgctt
    26401 acgcaacaag ctcttgacaa ttcatgctct aaaaaatgat agataatcaa gcagaaaaag
    26461 aacaaattat gctatgttac atcaacaaaa agacatctca tatcttgtat gaaatgcctt
    26521 ttttatgcaa tttttctttg tcgcatcaag gttctttcaa tcgtagtaaa ttagtgataa
    26581 atttcttttg tattttcatt cacattgctt aatgtccctg tgttcatgcg gataatcaga
    26641 tttttaatct ttttttccag ttaaaaaagc aggagacctt ttcagatttc ctgctcagta
    26701 gagttgtgaa gatgttattg ttgttattta atttcaatgt tcgtcatagt tactaaccca
    26761 agacgaacta aaatagtttg tacgatacga cacccaaaaa tacctagaat caggagtaaa
    26821 gcatataaaa taattgacgc ttgtaaatat tgtctgccaa agtgaataag aataatcagt
    26881 ggagacatta atattacaaa gaatataatg cttgtaatta ttttttttat gttttttgta
    26941 tctgccaaat caaattcagc tccacaagaa cattcgttag gtattcctcc ttttatttca
    27001 gcaccacagt tcatgcaata tagtttcttc attctaatca cctctacaac ctggaattgt
    27061 tctattatta tctttcaatt tctatactta aagattttcc agatttatcg ctttgaaaat
    27121 tttcataata ccaaaaatag aaaggctttt cacaaagttc aaaataaata atttcatcgg
    27181 gttttttatt ttctattttt gtattgtaaa tatcttcatc atgtacaaat ccatataaaa
    27241 aacgactatt gtctggactg atatttaagt cttgcaagct atcacttaat gctaactgtg
    27301 attttagtag cgacatcgta tctttgtcat acgggcggtc tttctttata acctcatgta
    27361 cacttgtaat ataagcatat ctctttttac catctacttc cttaatttta aattttgaaa
    27421 atgtcagact tgactctata tcgttttttt ctgatatata caaaacactt ttatagtttt
    27481 cgttttcaaa ctcctttata atcttatcaa tatgtgcttt atattcttcg ccttgttcaa
    27541 attcaataga ggcacttaac aaagcatcct gtatcgagtt tgcataattt ttatcagaaa
    27601 gtaaattttc ttttgagttt aaattttcta ttggataata ttttgttatt tcttccggaa
    27661 catatttatc gttcatttct gtcctttgga ttaaactatt tccacctgtt atttgaccta
    27721 aatatccaag ccctaaaaaa acactaaaca caaaaataat tattcccaat attacccaaa
    27781 ttaccttctt tggtttactc ataagattaa gccctttcaa tttattgatt gttaaataca
    27841 aactccaatt tatatgtttc tctgtattta cattatacca ttttccacta cctgctacaa
    27901 tgtttcaaaa tcttaagatt actatttttt ctgattttga aaagctactt atccgtccat
    27961 aatttgtaaa gggtgctaag cattgcttac gcaacaagcc cttgacaaat catatcccga
    28021 taagtttaga gtaatcaagc aaaaatgaaa tagtctgttg tttcaagcca atgaaaaaga
    28081 gataccagca catttatgca gtatcgctaa cttaatagca ttattctttt attcgcaagc
    28141 aggtaaacac aggttattcc ttaatatcct tttgtttggg aaactccagt ttcccattta
    28201 gatacagaag ctttagaaac gcctatataa tttgcaagtt catcttgtgt aattcctttt
    28261 tctttacgct ttaaaatcag cgtttttgcg atgtttaatt cattcatgtc aatccctcct
    28321 tatgtataat tatatcctta aacatgtaaa gctaacaagc gatttgaagt tgacttttga
    28381 cgaaaaaatc aaccacagag aaacaaataa acaagtatat aaaatatata tttaaattta
    28441 tttttaatat cttttgtaaa atatataaac tttgtaaata aaatccatat tatataaaac
    28501 taatagttaa tctatctaaa tacagttttt atttatcacg tttttaaata ttttgtataa
    28561 aacctctttt aatataaaca ccttattttc atttctcatt tcatattctt tttaaaatac
    28621 tgaatataac tcttcgattt tccatttaat tcttttataa agttattatt cgtatttatt
    28681 cctctaatta taacaggcaa ctatcatgta ttggtatttg tctattatat aaaaatctat
    28741 aaaagaaaag cagtcaatca tttagattaa ctgcttgtac atatatctta ttaaactgta
    28801 taaactataa tttttattat tctccatttt catcccaagt cataaatccc cgtccactat
    28861 ctggataagt atcaatagac ttttctctaa tcatataacc aattttataa cactcttttg
    28921 ttattttatt agatagtata cagccttctc catttatcta attccattta attaacttct
    28981 cttttcaacc ttaactttta taaaatcatc tttttaataa tttcatactt attttctctt
    29041 caacatcatc aaatgagggt gtctcaaaat taacttttta cttcataagg cactcttttt
    29101 tgtatgaagt aaaatatatt ttcatcattt caacaataag taagaggctt atctctattt
    29161 tgagacagcc ccatttgatg ttgttgaaca tttaaataag gttgaactcg ttttatctct
    29221 ctttttaaga tatctgtaaa tttgcacata aagtctcttc ttcttttaca attctaatag
    29281 ttttattaaa gccaaagtat atatctccat attctttcgt atatgattta ttggtactct
    29341 ttcatctact ccatgaccac cagttttata cgctaagaac ttaggtccaa acgccactat
    29401 atctttaaac caacttgcat atgtgccacc acccatagca attgtatatc cattttcatc
    29461 tgttacttct tcataggctt ctaataattt tttaagtact aaattactct catccacata
    29521 atgtaagtct ttatgagcaa taacctctat tttccaacca ttacaaaaaa cctttttgaa
    29581 tctatctatt atattgttta aaactatatt tttaccatac cttatatcta actcacatac
    29641 gattttttcc tcatttactt ttaatattcc taaatttaag gtagtgtttt tgaatacctc
    29701 atcatattga ttaatcccca atgcatctcc ataataatcg ctacaaaggt attttgaaac
    29761 taattctata tcttctctat gctgaaaatc catattatct tcaactatct ttattaccat
    29821 tttagatata gcattttctc ccttttcagg attacttgca tgtacagcta ctcctttagt
    29881 agttaactct gaaataattc cattaataat ttttgcagta caatgagatg gaacaacatt
    29941 ttctttagtt cctcccctta ctattatgta aggtttactt ttgtcaattt taatttcttt
    30001 ttctatcctt atatgaaccc ttcctttttc tccattaacc actggataca tagcatctgg
    30061 agtaaatccc atacttggaa tttcttcacc tttatctaag taatatttga tacatttcat
    30121 acctgtctcc tcatttgtac caaaaattaa tcttattctt ttattaattg taggatacat
    30181 atcctctatg tttttcaata catgtataag catagataca gcacccttat tatctatgac
    30241 acctcttcca taaataatat tgtcaattac tgctcccgaa aatgggtcta cactccaccc
    30301 ctctcctggt ggaactatat ctaaatgcat tggaatccct attaattcct ctccctctcc
    30361 aatttctgca tatccaatat aattatcaat atttttaaca ctaaaaccta aactagcaca
    30421 gtaatctaaa gttttatcta aagatttttt aggtcctata ccaaaaggac attgtgaaat
    30481 aggttcacct tgttgtgaat caatttttat caattctttt agaaattcta tggttctatt
    30541 taaactatta tctacacttc tgcttatatc cataaatcaa cctactttct atctttttaa
    30601 agcctgtcct taaagaacag gcttttagca ttaaaaatta tttttttgta aatactaatt
    30661 cacaaacttc atcatcatga gcacattttt gagcaaaatc taatttaaca ccttgtgcac
    30721 agtcaactct tccataatcc ccataacaag ctaatctaca aatttctttt ctttcttctt
    30781 tagttaatcc atattctttc caagcagcat ctaatggaca gtgatggaat tttaaaacgc
    30841 cattctcatc tgtattttct actaattcca tttcaaacac tttttgtcct ttactgttat
    30901 ataacatttc tgccattttt cctggagtat ctgctacgtt atatttttga cctctcattt
    30961 taccaaatcc atatattgct tctttacata tatcatctgg attagctcct aatttttcag
    31021 cttctttcat aagaagatag aaccatactg ctctttctcc tgttgctcct ctaattcctt
    31081 ctaaatattc cttttctgta tacatttttt ctgacatttc tttctcctcc taaaattaaa
    31141 ttctatattt taatcactgt gaattagaat attccaatga atgaccctac caatgctaat
    31201 acaactaaaa ttactaataa cttagttatt gtccacttct tgtatttcat tccccaatat
    31261 acgaagaata caacaccaag tggtaataaa cctggagcaa tcatatttaa tgtatcttga
    31321 acaacaattg ctttaccacc aatatcaaat gatagcggag ttgaaagctt aactgtagtt
    31381 gcagataatg ctcccatcat aaacattcct agaattgaag caccatcaat tatcttattg
    31441 acaataccac ctgatagaat tttcttaatt gaatctctac ctaaagaata tcctaaatga
    31501 aataagtaat aaccttcgca aaatagtaat atactaaaca ttattgggaa aaatgcacct
    31561 ataacacttc cttctgctcc aaatgaagca cctaaagcta agaatattgt ttgaaatgta
    31621 ccaaaatcta aagtatctcc aattccagcc aaaggtccca taagaccatt cttaacacct
    31681 gaaataactt ctcctgggat ttttccttca gtagctttct cttcttccat agctaaaact
    31741 gtaccgtgaa ttaatcctcc ccatatagct tgagtattaa aaaattgtaa gtgtctcttt
    31801 aacgcatcta ctaaatcttc ttttctctta tataactttt ctaaagccgg agtgaaactt
    31861 gcacaaactg ctaatgcttg cattctttca aaagagttgg ataattctgc tgtccaccac
    31921 catcttagat atgtttttgt tacatctttc tttgtaagta ctcttgtctt attaacttca
    31981 gttggacctt gtccattttt aacttcttta ttttttattt cttgcattat acttcaccta
    32041 cccttttgtc ttctttcata aaggtaatta aaagagctat acaaacacca aatattgcag
    32101 ctgcagtaat tgatatttca aaatattgaa ccataaaaaa tcctataata aacattggta
    32161 agaatttatt tttacctata gtaaatattg ttgttgcaaa acctaatgca ggtaatatac
    32221 cgcccattac tgttaatcca ttcattaacc attgtggtat aacatctaat aatgattgaa
    32281 ctaaatctgc tccaaaaaag ttacatacaa aaactattgg aaaccttaaa acaaacccaa
    32341 atattaaaga ataaagtgtt gcacatctcc agatgccttt tgtattacct tcttcagcat
    32401 acttatctgc cttatgaacc aatacagcat taccagttct tcttaagtta ttaactaata
    32461 ctcctataac acctaaaggt accgcaatag atactgcaac ttctgcatta acaccagttt
    32521 gaagtgctac tggtattgca ataagagcag ctagacattt atctgatgga atatttcccc
    32581 ctgctgcgac cattcctaga taaaccattt ctatacttgc accaacaatc ataccagttg
    32641 ttacatcact ccaaagtaat ccaaggctaa aacctataaa taaaggagac tttagtgttg
    32701 agaaaagggt ataccctgca agacctgctg caaaccaata ccataatcct gttacaattg
    32761 ccaacatcaa aacagattcc atttataatt cctcctaatt tatatttttt atttaaattt
    32821 ctttaacgca ttttcatatg tcatagatga ttcctctggt ataacttgta ggactatttc
    32881 aaccccattt tcattaagtt cattaagttg ttctacatcc tcatttccta atgaaacagc
    32941 tgtaaacacc attttctttc ctgcttcatt tggaatacct cctaattgaa tcttttcata
    33001 tttaagtcca gctttataag ccttatttgc catatcaaca tttttaaata gaataaatat
    33061 atttttatct cctaaagtgt tgttttggaa tgcttgtccc gcatcttctg ctgttaaaat
    33121 aactacttct actcctgatg gtgctgccat tttatatatg tcagccataa attcatcttg
    33181 acctaagaag tcatcaacta ttattatttt ttgagcttga gcgattttta cccattttgt
    33241 tataacttgc ccatgaatta atctgtggtc tactcttact aatgttattt ttgccattat
    33301 attcacctca taatttatat ttttttaaat tctttaatta aatccacaca aaggttatta
    33361 ttttttttga cgattctttc tggtaatgta tgtaatttat cttctttgct taattcttca
    33421 tctacgatta aaattgtctg taagctaagt ccatttattg catctattcc tcttcttata
    33481 gcaacagcta ttgcaacact tgatgtagat cctccatata aatctgatat tataattttc
    33541 acatcttctt tatctactac ttcttcaacc tctttcataa atacagaaac atctttttca
    33601 gaacttaatg ataaaacttc atattgattt gacaatccaa agtttttatt tgtttcttct
    33661 actaagactt ttccccaagt tccatggcac agtatgatta ccttatattt attcattgct
    33721 aaataaactc tcctttatag cgattaatat tttggtttaa ttctacttct tcttcatcag
    33781 gtaccatttg aattgataca ttaatattct ttttaataaa ttcatctatt aaaagaaact
    33841 cttttttatt aatgtataca ttttgagata ttttcttttt tcctattcct gtaggtattc
    33901 ttccaacaat aagttttttg atattaatcc cttcttctat cattctattt gcagtatcta
    33961 aatttttaat taatacaaaa acgttatcat cagcttgatt attataaaaa tatctccttg
    34021 attcacttac agttaatatt gataaatcaa tattctttgg tgctgccatt tttatcaaac
    34081 ctttagcaat aacatcattg gttgtttcat catctactac tactatcttt tttacaccta
    34141 gatgcttaac ccatttatat ataacttgac catgtattaa tctatcatca catctgcaat
    34201 attttactaa agacattttt aatcaaactc ctttttctat tacattgagc taagaacttt
    34261 attaatattg tttataccag tttgtcctgc ttcaatacat gcttctgcta actcttctaa
    34321 actttttccc tctctattaa ttaaagcttc aagtagcatt ggaagattta ctccagtaag
    34381 tgcatgaaac ttgccacctt ttttaaggtt taaacttgca acattacttg gcgaacctcc
    34441 taataggtca gctaatacta aaactccgtc accttcatct aattcattta acttttctac
    34501 aaaattttta tttaagtctt caatattatc attcctattt agtttaattg ctgctgttct
    34561 ttctgctggt cctgcaataa gttcacagct ttttataatt tcttcactaa aactcccatg
    34621 agtcataact attattccta tcaccaattt cacctcactt ttcttgtcac tttttatata
    34681 agcaattgcc gtgccaactt atgtgtatca ataacaaatg gctattttac aggatttaat
    34741 caaaactttt tactcaattt tttttaaaaa cttattattt cgatatgtat caaaagattt
    34801 tttcaacctg tatcaaatct gtatcaaaaa aagtaaaaaa aagatagcct tttggctatt
    34861 tttaaatttt gtatcaactt ttgaatttat ctgtatcata ttgtgattca aatatatcta
    34921 atatatttgc atattccata tctgaaataa ttatatcgaa tgtctcttct actagcttaa
    34981 aactttcttt tataactaaa tataattctt tatttctact aattctttct tcatatgatt
    35041 tataaactaa agactctttt attattactc tttcaatcat acatgaacaa tgaaatataa
    35101 actttactaa aagttcatct tgaattacta aagaatagtg ttcactaatt ctattaatta
    35161 cttcttttaa tataaaataa gccttttccc catttaaaaa agtcaaagtt ttactaatat
    35221 tatcaattag tagtctatca aaatatctac caccattatc tttttcagca tatgtaactt
    35281 ccatatctcc ataatgtttc tttatagatt ctataatatt aaaatataat ttctctaatg
    35341 tcatttctgg agttagagcc tttctagttg cttctattac aataggtgta cttaccatat
    35401 ctattgcttt tacttctttt cctgtagcat cacgaataac tttagcaaat gttaaaatag
    35461 accccatatc agttaaaatt aaaattcctt ttcctttatc aattttattg gctatttcta
    35521 cagcttgtac taaaatgtcc tgaactctat cttctaatcc catattaatt gccactgcat
    35581 ggttacaatc cagtaaagta ttggctacat tcaccatgct agatgctgta gattcaccat
    35641 gcattatgac taatattgct atactctcct catttaatcc cacatacgat aaataaagaa
    35701 atgttgctaa aaaaattgct tcatcttctg gaaactctac tccatacatt aatgacatgt
    35761 tagataatat ttcttttgct atgttatatt catatgctct ttccttcata atttcatctt
    35821 tattaggatg ctttactata tttccaatct ttattctttc aattaaattc tgaatatgta
    35881 aagctatagc ttttattaat ttatctaatc cttctctttt gctccattta tcatgctttt
    35941 gtaaaatata ttttacagta gttgcaattg aatcattaac aatgttgtta taagctgttt
    36001 gattactact attatatata aaagtattca ttaaattata ggaatacttc tgtatgtctc
    36061 catctataac acttctaatt tgtgactctt taataccttc ttgttgaagt ttattccaag
    36121 tttcttttat cattccataa aaatctaaat catatttttt attttctatt aataaatttt
    36181 cactttcaaa atccttttct tctggtaaaa aaataatatc atcttttcca ataaaactga
    36241 aattttgtac aaactcctct tttctattat ttccatataa tgattctcta atattatttg
    36301 gtagaagaga gagtcttaca tatacacatt cccttttata ggtcatataa tctaaaaaag
    36361 ctcttgcaca aattaattgt atatcgcttt ttaattggcc tatgtttccc ttacacttat
    36421 ataaaataaa tgccttaata acttcttttg aaacctttaa cttaacacca atcctaacac
    36481 tctcttcttt aaaaaaataa catataagct tcattctctc ttgaaagcct ctttcctcta
    36541 aagacggaat ctttatagtt acaggtattc ttcttaaaaa cgtttccaac attgctgttt
    36601 ctgggtcttc tgtagtagct gcaattatca taactctaca ctcgtgtacc ttattagcct
    36661 ctcctaacct tctatatatc cctttatcca taagcagaaa tagcatttct tgtccctctg
    36721 cagacaatct atgaacttca tctaaaaaca atactccatt attagcttca tgtacaatac
    36781 catacttatc tttatcagct ccagtaaatg ctccttttac atatccaaaa agctgcgcta
    36841 ataataattg ctgattatca gaataatcag cacagttaaa tatgataaat ggtgcattat
    36901 cactatcttc ttttatagtt aaactatact tatacatata ttctgcaaat atagatttac
    36961 caactcctgt aggtcctatt attaatgtac tcaacccctt aggtggatat attactgctg
    37021 ctttagcctg ttctatttgg gaccttaaac ttccatcaca tcctactaaa ttaaaaatat
    37081 tgttctcttt ttttatactc tctaaaatat actcatcttt gatagaatca ttttcactat
    37141 attcgtttat agagttaata ttatcaaata tccctttatt tagtttcttt cctaaaatac
    37201 tctcaatact ttctttatat aagtactgaa caggcttacc ctttatcttt ataataatac
    37261 tttcttttat taattgattt aattccttac tagcattact tctatttata cctacttcag
    37321 tagctatata ttctgcatca aatccaaatc taatgccatt attaaagttc aagtgttctt
    37381 ctgttaacct tttcaagcaa attaaaactt tttctttcct tgatagcttc ttttgtgtca
    37441 tagttaacct cccctataag cagtaatact gttaaacatt tattattaag cttaattcta
    37501 caatatttta atatttttgt ctaattatgt aactattgat tttctttcct attcgtttta
    37561 taatataaat tacgttaccc tcatctttta tagaggagga atttatatga attttttatc
    37621 agatttacac acccactcta tagtcagtgg tcatggctat agcactttac tagaaaatat
    37681 taattattgt aaagaaaatg gaattaagat actaggtaca tctgaacatg gtcctaagat
    37741 gcctggttct ccccacagat ggtactttca caacattcaa aatatcccaa gaatcataaa
    37801 taacattata attttaagag gctgtgaagc aaatatacta gatattaaag gaaacataga
    37861 cctagagcct tttgtaattc ctcggttaga ttatcttatc ttgtcatttc acgaagctgt
    37921 gttttctcca aatacattag agaataatac taaagcttta ataaatgcaa taaataagca
    37981 tgataatatt gaaatattag gacaccttgg aaacccaaac tacccaatcg actatgaatt
    38041 aatcattaaa ttagctatag aaaagaatat cttaatagaa ataaataatt gttctattaa
    38101 aggtgtgtca agaaatggta gctcggataa ctgtaagtat attgctacac tttgcaaaaa
    38161 atatggtgct aaaatcatac taacttcaga tgctcacatt tgttttgaca taggtaacta
    38221 tgagtattct gaaaatatct taaaggaaat taattttcca gatgaattaa taatgaatta
    38281 tcctaaaaaa cttattaatc attttcacca aaaagataaa ttactagatg ttgattatac
    38341 aaatatctaa gtaataatac atgcttactt taactcccat aaaaatttta atactattta
    38401 tattatatga aataaagaaa ttataataaa gaagctattt aataaagctt aattactttt
    38461 atgaacttga tggattgata tggtctatga tagcaataga aat
//
LOCUS       DAEMHW010000015        37271 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEMHW010000015 DAEMHW010000000
VERSION     DAEMHW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37271
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.015"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..165
                     /locus_tag="KPK67_000651"
     CDS             <1..165
                     /locus_tag="KPK67_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBG2457656.1"
                     /translation="IEDEEKIPGIYKTVEQIVKVDKNTIKDFIKKGHEVEGCRIEYGT
                     TLTIPKAKKE"
     gene            175..789
                     /locus_tag="KPK67_000652"
     CDS             175..789
                     /locus_tag="KPK67_000652"
                     /inference="COORDINATES: protein motif:HMM:NF016302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBG2457657.1"
                     /translation="MESNNIYIKLMNVRVKFSKLNLKKSGENKFANFKYFELADFLPQ
                     ATGLLEEAKLCPIVTFTNDYATLTLINGENPTEQIVFTSPMRDLQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITENDSFDAVSGKEESKSNSNNNVKQSNRILTDKQLTRLYAIAN
                     SVGVNKALLKEQVFKQLGKDIKDLNKSEYDAVCAAYEKQAFKGA"
     gene            1521..1640
                     /locus_tag="KPK67_000653"
                     /pseudo
     CDS             1521..1640
                     /locus_tag="KPK67_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            1689..1946
                     /locus_tag="KPK67_000654"
     CDS             1689..1946
                     /locus_tag="KPK67_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457658.1"
                     /translation="MIIIRSQDKTDLVKADWINVDKEYVYAMFGEINNFKEIGKYEDE
                     KRAIQVLNEIQKFIENGVRTDYIDSCRVRHNQEKVFEMPVE"
     gene            1963..2343
                     /locus_tag="KPK67_000655"
     CDS             1963..2343
                     /locus_tag="KPK67_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457659.1"
                     /translation="MAKVWVDAGELLEKTIDIEDMFELNLRAIRKRNEKIKNENGEQA
                     KNEESEMETTNRGGPGKPIKVFNINTGEVKILKNAQEASRYMKVSCNHASYLARENKS
                     TKDGWKAEYIQGVTDGISKCGTSN"
     gene            2312..2452
                     /locus_tag="KPK67_000656"
     CDS             2312..2452
                     /locus_tag="KPK67_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457660.1"
                     /translation="MVLANVEQVIKLAEKILNKKKCSVNKAIDIAIKILSKYECEGML
                     EK"
     gene            2449..2622
                     /locus_tag="KPK67_000657"
     CDS             2449..2622
                     /locus_tag="KPK67_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HBG2457661.1"
                     /translation="MKLKDIIKLGEKYCYCPNCGNDKIGNDEGKLIVEEHTYYRECSC
                     GFNILIDDRKDEI"
     gene            2622..3074
                     /locus_tag="KPK67_000658"
     CDS             2622..3074
                     /locus_tag="KPK67_000658"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457662.1"
                     /translation="MNILASLLLIGISFIAGRIYEYRLNLKECENCDNKGGSKMSKVI
                     QCDFCKEIFGENNLECIELYKKNVENEMINIDKHMCPACYEKFVGEKVEKKITNFEKI
                     TRDKESLINFIVKCDAECDCCIYANDDNCSPISCTKGCEKWLDMEVEL"
     gene            3240..3458
                     /locus_tag="KPK67_000659"
     CDS             3240..3458
                     /locus_tag="KPK67_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457663.1"
                     /translation="MSKYVLRWQMGLLLENIKIHYTYGSKEMLRQKAEVLAKDDKILF
                     ITIDKIDEVIKDTRSQKMAEYFEDEGVE"
     gene            3458..3649
                     /locus_tag="KPK67_000660"
     CDS             3458..3649
                     /locus_tag="KPK67_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457664.1"
                     /translation="MIICVMVKTNKKGSYVHGEIKVDDDDLKSMSDEEKSEHMRKIAQ
                     EYICKNLIDWYWFGIGENN"
     gene            3663..4664
                     /locus_tag="KPK67_000661"
     CDS             3663..4664
                     /locus_tag="KPK67_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG2457665.1"
                     /translation="MIIHKFIIHVLDKNSDMPILNDFEGRVSQDIEAFFNKKISKVSR
                     DNDIRTAVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYTEKDEKKVAILKLDYNKSYTHSISFEDDKFNIQMFKNEINIQETKAIKIGAIIG
                     LSGMNDEYHLRVLDKDAEKEEANSKFVTEFLNATKVKDDKYKTKKFKECIDSMIAHIF
                     NGDVKQAEEMRRTAIYMLREKQKLDINEFADKVIKDDLKESFKDHAEEKGLDESFNID
                     KKWVEKNLKNRHIKTDTGFEIKGKMDDFEDPIKYTVRQNQNGYIDIIIKNVTFYEEK"
     gene            4673..4855
                     /locus_tag="KPK67_000662"
     CDS             4673..4855
                     /locus_tag="KPK67_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457666.1"
                     /translation="MTNKEMCKSKNLDEREVYKEFGKEICGSCINDKRDCESKDCDTA
                     HKIWLEKEAISYVKHL"
     gene            4839..5210
                     /locus_tag="KPK67_000663"
     CDS             4839..5210
                     /locus_tag="KPK67_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457667.1"
                     /translation="MLSIYKVKLKTKRTLEQVRNQSVDFDYSEEGLKNVLRYYNLIDG
                     LEVIVLKFRDEYCLANFNEEDEKTIMEAHYLLEQDEYTGCYINEYERFKRDWENGDCD
                     GEGYMVFSDDEVEIIEELRSK"
     gene            5289..5630
                     /locus_tag="KPK67_000664"
     CDS             5289..5630
                     /locus_tag="KPK67_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HBG2457668.1"
                     /translation="MANIYCENYNCKNYFEDMCMLERIEINNLKVCESYIEGKNELYE
                     LENGYTMHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEVLEEKLIK
                     INSHLVLEDLR"
     gene            5646..5876
                     /locus_tag="KPK67_000665"
     CDS             5646..5876
                     /locus_tag="KPK67_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBG2457669.1"
                     /translation="MIHELKILPQYFKEVVNGNKNFEVRKNDRGFKKGDLLILQEFDG
                     EKYTGLETRKEITYVLDNSDYMQDGYVVLGIK"
     gene            6012..6419
                     /locus_tag="KPK67_000666"
     CDS             6012..6419
                     /locus_tag="KPK67_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG2457670.1"
                     /translation="MKINFVIDGEPVGKERPRMNSITKRTYTPNKTKNYEELIKWLYQ
                     SKAKHYFEGYIKMTLRCYYSIAKSNSKKIKEQKRNNVLRPSKKPDIDNVVKIIADSLN
                     EIAYKDDTQIVEVVASKYYSDNPRVEVILEDIS"
     gene            6482..7213
                     /locus_tag="KPK67_000667"
     CDS             6482..7213
                     /locus_tag="KPK67_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HBG2457671.1"
                     /translation="MNDLKLIENEGIIKVYTTDEDIKVVDGRELWEGLGVKQDFSDWI
                     KTNLKSVDAIENKDYFTIPFKRERQILIEYTLKLEIAKEICLVAGASPRANKELKRNS
                     KNYRKYLIAVEEKYKASNNLTKTQLNQINDIVSNALYEMQTKHDAQIEQFKRESSQYY
                     RPTSKTKYDISSYIKDRLDIPRANEEFDLVKKRTLLILGADKWEDIPKDVLLKSLNII
                     DESIRIIKSERKTNQISFFEKDNFC"
     gene            7309..7797
                     /locus_tag="KPK67_000668"
     CDS             7309..7797
                     /locus_tag="KPK67_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457672.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAVGYDEKVSPTYAFNSTVENEIIKKERDIARLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKTCRCVRERLVYKVMDMLYPKIKENELPLF
                     KN"
     gene            8116..8871
                     /locus_tag="KPK67_000669"
     CDS             8116..8871
                     /locus_tag="KPK67_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457673.1"
                     /translation="MPEDAKLEIPNEIISTIVQCVNGAVGDKIKEDIKKQNLVTKNSV
                     PTRIWDLLNRDLRQALDSPDCMAYEAKRGCWEMVIIYEKSSENIFTIMREQRFNEIYK
                     KQPERRNMHYLNDLTKNFNEDLKAPIGQISFFPKKFDDEDKVKEHVQKLLENLIKDNV
                     VVKHHALILFNASEYQLTSIRAVMIDTNLDIVTEKDWSSYISIEESTVAEKVDNTNPV
                     ANNPTLGLNLTSKAVARKKTHPLKKAKKEEDAK"
     gene            8905..10053
                     /locus_tag="KPK67_000670"
     CDS             8905..10053
                     /locus_tag="KPK67_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBG2457674.1"
                     /translation="MEINFNGERLKKARIYRGMTVAELAERIDCQRQTVSMYENNKSK
                     PNDNNVVKRIARVLGFPVKFFLETGTNIAIGSTYFRALLTTNKKYRAEQIQKMEFLAE
                     IYFFLQDYIEFPTLDLPDCSGKTPEEAALLLREAWGLGLKPIDNIIYEVEQHGIIVTS
                     FSTSTDDIDAFSQMIDISGETVYIIGYSSNKTSASRIHFDIAHELGHICLHEWSEDVE
                     ALEKQEFKERESEANRFASAFLLPEETFKIDAKRTPLRISNYTELKRKWKVSIQAMIR
                     RSYSLGIISMDEYQSMIRTLQRRGLRKSEPLDDELLTSLPALLKTAVLMLLNEKVFTP
                     KEFMDELSFSYNFSLEPEEVEYLLSLPKDTLTSAKVIPFPDLQLKKGI"
     gene            10754..11431
                     /locus_tag="KPK67_000671"
     CDS             10754..11431
                     /locus_tag="KPK67_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HBG2457675.1"
                     /translation="MLKILQDKNVKVMWSKNGEEVWFNANDVGEELGIVNIRDTLRNI
                     DREYKKKFNESTVGDSYTRNFKDKLPNFGTTFVTEEAVYNMSFRSNKPEAKLFTKWVT
                     QTLKQIRIHGYYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVYYAISQGSNKPQMYY
                     KHFTELVRRKLGIPKGVKRDELNQSELFDIQALERIISMKLPKLIDKDMNYKEVYKKI
                     KELIEMI"
     gene            11863..12429
                     /locus_tag="KPK67_000672"
     CDS             11863..12429
                     /locus_tag="KPK67_000672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457676.1"
                     /translation="MLNQFNEFLGNYINIISVIASFASIAALIISIIAFMESNKQAKL
                     ILKYRRNNEDNMAGDYYENGNGYIRLDLNKIDDKSNVYAEIRSNNLVDFVIENKSKIV
                     AKSPILSLKFINMEVDIEENNNFEQINSELRWHPKDNTTIHKGINFRIEQFNFKNCVM
                     LKKEAYIEVVLSADNMETKEFSIPIKAC"
     gene            12488..13180
                     /locus_tag="KPK67_000673"
     CDS             12488..13180
                     /locus_tag="KPK67_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2457677.1"
                     /translation="MQDVKEKVKKDYLKGMKHKEISAKYDISLNTLKSWIKRYNWSKE
                     KKKGAPINKRGAPFSNKNSVGYGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENSEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKSEVDNLSKDDTDKEIT
                     VRVMKASGENGK"
     gene            13170..14522
                     /locus_tag="KPK67_000674"
     CDS             13170..14522
                     /locus_tag="KPK67_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG2457678.1"
                     /translation="MESKFISNNHFYDFIFNWDYKFYFLVGGYGSSKSYHVASKLIIK
                     LLKEKRKALVVREVYDTMRDSCFSLLEEVAESMGLGDVLSFKTSPMRVIFPNGSKIIF
                     KGMDNPAKLKSINGVSIIWLEECSEVKYTGFKELLGRLRHPSLSNHIICSTNPIGEDN
                     WTYKHFFRDEEKDRFVLDDTVLYKNRIVTKNNTYYHHSVADDNFFLPKSYIEQLDEMK
                     EYDYDLYRIARKGRFGVNGTKVLPQFEVMEHQEVINKISNIPQRYYKVGFDFGFEKSY
                     NALLRLAIDHENKYLYIYWEYYKNQTTDDVTVKDIKEFKDTQELIKADGAEPKTIKYF
                     RQQGFNISGAKKYAGSRLQNTKKVKRFKKIYCSSECKHTIKELKNLTYATDKNGNLIY
                     DEFNIDPHTFSAIWYALDGYEVSDIKELKYNNDIYNKGLGLKKNNIPNQYNKKGGAVF
                     "
     gene            14522..16045
                     /locus_tag="KPK67_000675"
     CDS             14522..16045
                     /locus_tag="KPK67_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019278964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2457679.1"
                     /translation="MSIKNILLNLDEKELKDRKHAERDFLFYLGECRNKTMGLLDDDF
                     LGQSWITFDNLDYTPSQIIDNKVKPLINKQARFMFGKEPTIILKAYEKEHKESCEELR
                     QYIDSILNASKFWSNTLKAFKIATITKRVLLRLEAEPNQPIRLFYHSIDDFKYQVDSN
                     DITKLKSVIFVRFDASTIKEVTANQIWYRYTYYMKESNVNNQESCFLRVEKFKGDNLS
                     KPIEVKESDTKLSKIPCWVIVNEQSITNITGISDIEDLKPLQDTYNKRLSDFNDSLKF
                     LMFGQTVVIDATEETVNACKIAPNALMALKTLEEGSEKGKQAQAYRVESSFSNADPVN
                     SFFKRLEDSMYEKLAIPRPEQLQNIPSAKALKYLYTELIARCSEKWNDWEPAIRSMLR
                     LIVEACSKFNCYDDWNHDWDDLMFSIVLNKNYPIPEDEEDSKRLALEEVNNNVRSHRN
                     YIKEFGDDEDYEEAFNEVLEDNEKIQSVEQDQFRKDAEIEIDNIDEELNNKSDNSGK"
     gene            16058..17647
                     /locus_tag="KPK67_000676"
     CDS             16058..17647
                     /locus_tag="KPK67_000676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457680.1"
                     /translation="MKDNAYTKKVLEARKKLLLLDKKAQLEILNVYKDASKIILNDIV
                     KNKELNLSSKYLKKLNKSIEKYINELNQRLVPVAEKSITEASNIAKDLQMYYYQSIVP
                     NKSINLACDAMCIKTTTNVVEKIVTGNFYKDKRSLDSRIWGYSNKNRKDIDRLIKANA
                     ARGANAKTLAKSLDNYVNPVRKTEAKTLEVGMNKSISYQAQRLARTSITHAFVETSVQ
                     NAINNPFCVGLQWNLSSQHYIRQVKWRGEDECDEYAEQNRYGLGEGVFPSEKYPIPHP
                     NCLCYPTQVIVPINEASKFMNDWLNGMDNSNLDSWYDEINTETTNLYIPKIKYSSNDV
                     KSKYGKTLNKKINSFESKIENTSSKYKKYTTGVKYEVTNELKTAFAYSIKDDIIKINP
                     LSPIFKEYNFELSMMHELGHRVDLKELEIYKNIEFKDAILKSSNYVLNNLKEIQIEYD
                     YLINLNDSPFLSDILGALSFNKIEGLLATHNITYWQKQGNIEKEIFANLFALDYESDK
                     HILSFIKKNIPDIYKVFNKII"
     gene            17660..17830
                     /locus_tag="KPK67_000677"
     CDS             17660..17830
                     /locus_tag="KPK67_000677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457681.1"
                     /translation="MGEQMPIMKRLRNDKELLELRRLYKEKYNENAPGFNYDEYSSYD
                     EYKEKLKELIQK"
     gene            17898..18551
                     /locus_tag="KPK67_000678"
     CDS             17898..18551
                     /locus_tag="KPK67_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG2457682.1"
                     /translation="MLEYFKKLLGDEEGQKVYEKLSKDKENKLLLDNIKSPRYVEKTE
                     LENANKEIKEYKKQIGDRDKQLNDLQGKVKDNKELSDEIESLKNANKEIKENAEKEIE
                     VLKFNTAFERAIESYNPRNPKALSALINKENINFVDGKIIGLDEQIKAYQQSDSYLFN
                     NEKNKGDDENVGTGGLEGGTTSLLDNGSEISSIGELLANSKVKSENIEAQKKFFGEE"
     gene            18563..18919
                     /locus_tag="KPK67_000679"
     CDS             18563..18919
                     /locus_tag="KPK67_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457683.1"
                     /translation="MSIEKSEIYMGENKTILKFAGNLFQNVNIKVKKTDVAVVDGKRI
                     LKAGTLISKDGKLVDGTTVTNDKAFGLVYRNIDFTHSNGNESIPVLIFGFVDEKTLPS
                     VIPEEAKQAMKMIMFL"
     gene            18943..19971
                     /locus_tag="KPK67_000680"
     CDS             18943..19971
                     /locus_tag="KPK67_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBG2457684.1"
                     /translation="MDWKDFIDSKEIAKYIKKLPLEMLIGESLFPRKKQIGMDLKYIK
                     GAKKKPVVLKQSTFDVAVKIRALKAQIEVKSKRMPFFKESVLVNEEDRQQLLLASKAQ
                     NKELLLMIISQIYDNYLALVDGGDMQMERMRMQALADGVINIVSEDGDLVFDFEVPSN
                     HKEVLTGSATWDNPDADIIGDIQRWMRIMRDEGKPLPKRMVMTSKTFGYFAKNKAIKL
                     DIDKDGRVILTDEMIKNYLKNKVGLSVAIVSGTYKLEDESEESYFPDNKITFIPDGDL
                     GKTYYGTTPEEADKVYGSKLDCSVVRTGIAITTMRLIDPVTVQTKVSQLGMPSFERAD
                     ECFFATVA"
     gene            19988..20185
                     /locus_tag="KPK67_000681"
     CDS             19988..20185
                     /locus_tag="KPK67_000681"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457685.1"
                     /translation="MAKKKDNLQVKALVYLKYDNDCYKIDDVFEIRKIDQELMEEKGY
                     IEVIGEVEEKSNNEILNKDGE"
     gene            20188..20505
                     /locus_tag="KPK67_000682"
     CDS             20188..20505
                     /locus_tag="KPK67_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457686.1"
                     /translation="MAINNLDKLKLNLQEEEYPYFADEQLVVLLESNENNVLKASWRG
                     CLLKGATDDCIKIGPIETKSSNSSYWLSLAEIYKTDYLEEKSKNETTNTGYKTSMTRV
                     DGQ"
     gene            20502..20885
                     /locus_tag="KPK67_000683"
     CDS             20502..20885
                     /locus_tag="KPK67_000683"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457687.1"
                     /translation="MRKLRADKIIKTVNRGIALNPQTITIEQEIKQIVDGAIEITNEI
                     KELTVVIYPEKTNDTVINSETIGTAYKNKNFGMVADKEAGLRLNTESDITFKCIEGTM
                     KLNYVNPIVVEEQVCGYICGLEKLD"
     gene            20904..21308
                     /locus_tag="KPK67_000684"
     CDS             20904..21308
                     /locus_tag="KPK67_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457688.1"
                     /translation="MFTKAINEIDRKKATMPLLCMNIAFMLEGEAKNSAKWTDRTGNA
                     RQGITGTSLGGGNQYIVRLGHGVDYGTVLEEGSAPHVIKPRNAKALFWNGASHPVMKV
                     QHHGTKGTHSLKTTVSRNMPKIGKLIEGHWSK"
     gene            21310..22134
                     /locus_tag="KPK67_000685"
     CDS             21310..22134
                     /locus_tag="KPK67_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457689.1"
                     /translation="MRAGIRKTLIDNILRLKDCYEPTVPNKKTIKPYAVIVQGEDSDS
                     EGEVIGFRRTINIWIYEKRTTFNKLDELTKEVIEILDFKTITDDTTNEVFTCIYEGAV
                     GQDVIDEEWEAIIRCLRFSIIALDDKEDITNDRWVESLSKYTKDLLEIEVYKDNWKKN
                     FIAPCCLWRTANVESKRINYHLIEITKTMKCHVVSKNKDEIVKLLEELETKLIIDKRV
                     RLREDKNMYLTLVSVVEDRESDMFSNGQLTVVFKMIGKIKREGPIMNEIYNSGNLR"
     gene            22147..22350
                     /locus_tag="KPK67_000686"
     CDS             22147..22350
                     /locus_tag="KPK67_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457690.1"
                     /translation="MAETINKKTNISKQEEKYSKEDFLKNSEALGYKKEVVAGALFNC
                     KKKELTKVEFEKLIKDFLEREVK"
     gene            22354..23775
                     /locus_tag="KPK67_000687"
     CDS             22354..23775
                     /locus_tag="KPK67_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG2457691.1"
                     /translation="MATGTWNEKEKKEIPGFYNRFKTQAEKSTNTGLKGRLAMPVKAN
                     WGDVGKVITIKNDLKQLKALFGDDISYSAYKLGKLALLGNVKELLLYRLVDGNQKNGT
                     LILKDTTENTAKDVIKLETKYPTARNFNVTIKSNLVDSDKKDFLFFEGTKQLFSSSIK
                     GTIDEIVLEINSNLDNEYIVATKVADSDTTLANLVNVALEGGNDGCTSITNESYLKAL
                     EEFERYSFDGFSLDGVAEEALQETTKAWVAKNKELGKDILLFLGGKKEDNIKQINDKS
                     KSFNDENVINVGSSVYYEGIKYTPSEVAVYIGALAVSKGITGSICNAKTIFEEVEPRL
                     SQSEVKECLKSGTLILDFDDGDVIIVDDVNTFKKYTDDKNEAMGYISNIMFINTINKD
                     TSLKRKEFVGKIFNDATGQTTVICALKKYFEELMSQGIISEFNVDIDTELQATAKADE
                     FYWKWDAIKVDVMKKIYGTGYLG"
     gene            23790..24131
                     /locus_tag="KPK67_000688"
     CDS             23790..24131
                     /locus_tag="KPK67_000688"
                     /inference="COORDINATES: protein motif:HMM:NF020949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457692.1"
                     /translation="MSKYNDDYIEEANFLNGSNVRIIIDGEEEMYAEEIKADYEQDEQ
                     SVKLLGCEDEISRGGTKKGSFSLTRFKVNSNILKLGFKTFEIIYELRNAETFGYERVR
                     LKNCRLKKYLS"
     gene            24290..24787
                     /locus_tag="KPK67_000689"
     CDS             24290..24787
                     /locus_tag="KPK67_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457693.1"
                     /translation="MLDMDKRELENEEIEKEDYDLDEEIEETNEDRLMMKEDEIIARL
                     LEDTPIPKRTVFLERLGIPITLKALTEKEISKIRKDCTKISKVQGRREEKLNDDEFNL
                     ALIEKGTLVPNFSNKKLLDAMKVSDAKEYIKRKFLAGELSKISDQILELSGFYDEISD
                     DDIKN"
     gene            24988..25863
                     /locus_tag="KPK67_000690"
     CDS             24988..25863
                     /locus_tag="KPK67_000690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457694.1"
                     /translation="MYHIDVVISAKGDGETKSKLSAMEKYTKQTEKRMQALNRIKVNP
                     AIKATDKASSVVNRVNNNMNKAKKTVTARIKATDNASSVANRASNNVNKARKTVTARL
                     KATDNASSTVNKVNNKIKEVAKPVPPIIIRGQDESSSIIDKVKAKIQNLKADTIIKIK
                     SQADEAINTISRTKNKLQEFVSKRYEAAVKIRDEASSALGGITGKIDSFVSGAISKFA
                     QLATMAGTLIGGIGVGSAVKGFATFEQSMKNTQAVSGATGKEMEALTAKARQLGRETS
                     FTAKDAGDAMYYMGI"
     gene            26121..26360
                     /locus_tag="KPK67_000691"
     CDS             26121..26360
                     /locus_tag="KPK67_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBG2457695.1"
                     /translation="MAGWKSEQMIKAIPDVLNLAAAGGTDLALTSDIVTDGLTALGMT
                     ANDTTEFVDVMAATITNSNTSVELMGEWIAHAKAI"
     gene            26965..30039
                     /locus_tag="KPK67_000692"
                     /pseudo
     CDS             26965..30039
                     /locus_tag="KPK67_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            30121..30921
                     /locus_tag="KPK67_000693"
     CDS             30121..30921
                     /locus_tag="KPK67_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457696.1"
                     /translation="MWQKFKSLNTFLKVIIGIIAICFLPITLAVFSIEFLMKAIEAKN
                     KGKIVLGCFLALLTLSCARTVYLTKDLDDKNSTQVNQVSSNKDKEDAEKKAEEETKKQ
                     KEQKAQTEKQKKDEEAIKLVEQQKKNEDEQKSLENKKNNEPTKFFNDVYLKNKNCVNM
                     ASYEGIKSNIDIFGYKYKIIEPNETTLRQIDVYDNQSNDTVQFQFYENDTGEEVVTVM
                     QYNKKDEPDKSVAISDDFHIYPPKFNNHINNTNTEVNSIEEQINFMFN"
     gene            30990..31661
                     /locus_tag="KPK67_000694"
     CDS             30990..31661
                     /locus_tag="KPK67_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG2457697.1"
                     /translation="MVIDIYLKNEKEKIDFHFPVNPQDSLSIKKEKRFETVDIVNLGE
                     FDIKKEGEKIREISFKTFLPNLYDASYCRYSELKNPIEVVAMLEKWVDQAEPLRLIVT
                     GFGYNGLVTISSFSNTQTAGREEDRDIEITFRTYRELKIETLKKETKSNTKTDLKDNR
                     PNTQTKSKIYTVTSTDTLWSIAKKFLGKGSRWKEIYNIPENKKVIGKNPNVIKKGQKL
                     VIPSK"
     gene            31658..32620
                     /locus_tag="KPK67_000695"
     CDS             31658..32620
                     /locus_tag="KPK67_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ"
                     /protein_id="HBG2457698.1"
                     /translation="MKIILNGKYDIANFNEGITLSEAIDGVAYKMDVSLIETKALQDI
                     NIKKGDKIVLIDIAYESKKEETIFDGVIWETRRSEKSKKLTLSCRERTVYMEESEEQY
                     SFKENTATQRIEYYCKQWNIPYYNLANTGKKLAKVIHKTNILDMIKKDLKETATKGGD
                     LFRVRMDNKLKLFKLGTNANVYKLDSILEDANFTSSFNDAVTSVKVLGKSKDENTKAP
                     VIGTYKKDSDKYGTLQKIKQDEKIKNAKEAKKAAEAMFNSGEETISVDCAVDINRIRA
                     GDKVSLKGKEYYVIDVTHTLDSTPKMKLNVGTLDYIRRKFYTND"
     gene            32613..32978
                     /locus_tag="KPK67_000696"
     CDS             32613..32978
                     /locus_tag="KPK67_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457699.1"
                     /translation="MTDARFNGVARILKEKMNKSVANGTFGMGCELAEITAKGLKVNG
                     YKDEIQDYLVLESLILKEDYFTLSDEILGAEYRHKHKIETPKELKPLSIGDKVLVAIM
                     GAEFLVIGRVVNAKPISAE"
     gene            32953..33411
                     /locus_tag="KPK67_000697"
     CDS             32953..33411
                     /locus_tag="KPK67_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG2457700.1"
                     /translation="MPNLFPQNETFETVELKNNNKNELDLKGSFLFDFIKGEFVKNAD
                     GTLKRCNKVEAYKQWCQKAILTPRYKRSAYSSVYGSEIKDLIASNLSQSAKELEISRL
                     IKETILVHPYTKEVSNFIFNWLENSSLVNYEFDVLTIDDENIVIDGNIKR"
     gene            33420..34556
                     /locus_tag="KPK67_000698"
     CDS             33420..34556
                     /locus_tag="KPK67_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG2457701.1"
                     /translation="MERELPIPVFLTEDEEAIHERMLSNFQDVSILEGDFVYDATRPT
                     AEQIAELKQLGLQNNLKIAFPQTSYGEYLEWLGECKGVFKNQPTKSVGVITFIGIQGT
                     IITKGTIVTTIATDEKQSIEFELLETKTIGENETVDIKAECRETGTIGNVSQNTVTVL
                     LGSINGIKSVNNKEDFKGGTDIEDEEHFRERVLVAEQEDKLSGANSDYIRWAKEVDGV
                     GYAYVVPEWNGAGTVKVLILDKNRKAATQELIDKVQEYIYPLNVSEEENRDGKAPIGA
                     LVTVVTPETLLINVKASFIFSNSFSEETVLNNLKAKIDKYLDKIDLGGTVSYNAIQAI
                     VGSMMLTDEGIQDFSNLTINDVKENIKLQDQVVGVGEITNEVVG"
     gene            34553..35200
                     /locus_tag="KPK67_000699"
     CDS             34553..35200
                     /locus_tag="KPK67_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBG2457702.1"
                     /translation="MIASKKGREMLLTLSPIYEQSLIMNSIYEAIGSEFDNLELLNKE
                     IELQLFPQSATWGLEFWENRVGLSTNLDEAIETRRRKVIAKLQSKYIMTPKRMSMILQ
                     SYTGANIKINENIFPYTFSVELTSTQGFPRDLEDLYKRVNVIKPSHLSVSYKLVSLLK
                     SKTYFAQTTIMSEEITVYPYSSKEVKANVKAKFALAHNMSSEILTVYPKGGGING"
     gene            35193..36038
                     /locus_tag="KPK67_000700"
     CDS             35193..36038
                     /locus_tag="KPK67_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG2457703.1"
                     /translation="MADEQFYTILTNIGKAKIANAGMLGKSVVLEKIQAGDGGGSYYN
                     PTEDQTALKNKVWEGNINAFDKDENNPNWIIATACVPGSIGGFTVREMALIDNEGDMI
                     AVCKSPETYKPKVGNGAMKDLYLKFIIEVSNVEKVTLVVDPTAIFLTKKDEEKILTNI
                     NKLDTKIDTTKTELTSNIETTKTELNGKIGDTTLLETTDKTNLVSAINEVKTSVDSIE
                     TTAEKTTIKDTDNLFESDNVEEALNQLARNYNTLSEKKNNLETEVNGQRIKGISIANN
                     LIDMI"
     gene            36052..36690
                     /locus_tag="KPK67_000701"
     CDS             36052..36690
                     /locus_tag="KPK67_000701"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457704.1"
                     /translation="MTEKLTENSSLSELMIAFESIKNDLQIGKNNVASTLGSPFTGTD
                     KLGTTKTKIETLKNVLVETINSKNVSATSSETFTNLIEKVNWIFQSIEIFSLKNRIQA
                     TIVNTPSIVYNEVSSIKGTLRFTGKLQASKMRADYATAKIELVCGNRKEYFYVTDNTP
                     SASSTILTFTKDIIVEKGMDIKVQILLTSVGAGNLDGSYAARAEIEELKILR"
     gene            36700..36912
                     /locus_tag="KPK67_000702"
     CDS             36700..36912
                     /locus_tag="KPK67_000702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457705.1"
                     /translation="MKKQVYYNSLDEKEKIISENSNLYVIEIYETLNENYLVLSSSPI
                     EDEKLSYEELENELLIMTNELQGGLL"
     gene            36912..37094
                     /locus_tag="KPK67_000703"
     CDS             36912..37094
                     /locus_tag="KPK67_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457706.1"
                     /translation="MNINEVVVRILAERILNKGLNPLKNREFELDDVTNVEYRKAVED
                     YIIRESGVVEGAEPTA"
     gene            37135..>37271
                     /locus_tag="KPK67_000704"
     CDS             37135..>37271
                     /locus_tag="KPK67_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457707.1"
                     /translation="MNEELFKENLKRHEVTINKHNDEIDELKVANIESKAELKALCEN
                     L"
BASE COUNT        15569 a         3926 c         7074 g        10702 t
ORIGIN      
        1 atagaagatg aagaaaagat acctggtata tataaaacag tagagcaaat tgtaaaagta
       61 gataaaaata ccattaaaga ttttattaaa aaaggtcatg aggttgaagg ttgcaggatt
      121 gaatatggaa ctacactaac aattccaaaa gctaaaaaag agtaggtggt agatatggaa
      181 tctaataata tttacattaa attaatgaat gtaagagtta aatttagtaa gttgaatcta
      241 aagaaaagtg gagaaaataa gttcgctaac ttcaagtatt ttgagttagc agacttcttg
      301 ccacaagcaa ctggattact tgaagaagct aagctatgcc ctatagtgac ctttacaaat
      361 gactacgcaa cactaacttt gattaatgga gaaaacccca cagaacaaat tgtattcact
      421 tctccaatga gggatttaca gttaaaaggt tctaatgaat tacaagcact aggaggtata
      481 gaaacctatc aaactagata tttgtatatt cagttactaa atataactga aaatgatagt
      541 tttgacgcag taagtggaaa agaggaatct aaaagcaatt ctaataacaa tgttaaacaa
      601 agtaacagaa tattgactga taaacaacta actagattat atgcaatagc taatagtgtt
      661 ggagttaata aagcattatt aaaagaacag gtttttaaac agctaggaaa agatataaag
      721 gatttaaata agagtgaata tgatgctgtt tgtgcagcat atgagaaaca agcctttaag
      781 ggtgcataac aggaggtgat agagtgccaa tttttagaca gatatataca aatttttgga
      841 ctgacccaaa gatacaagaa gaattttcag tagaagataa gttattttat atgtatttat
      901 tgactaaccc tcatactact caaattggaa tatatacaat aactaaaaaa caaatagctt
      961 ttgaaatagg ttggacttta gaatctacaa atgcagttat ggacagattt attaatcacc
     1021 atcaacttat aagttataat tctgaaacta gagaaatagc aatcaagaat tgggctaaat
     1081 ataatctcaa tagagcagga acaccaatgg agaattgtgt cagaaaagaa ttaagagaga
     1141 ttaaagacaa atcattgcta ctactaattt atgagcatat agaaaataag aagttcaaac
     1201 agatatttga agaatatttt gacgaactcc gtaacggtgt acgtaacgaa gctcgtgacg
     1261 aggggaataa caataataat aacaataaca ataataataa caacaacaat aaagaggtta
     1321 cggtggttgt ggataaaatt aaaaaatatt ttgatttaga atctaaaaac attgaaaaaa
     1381 ttgttgatat attcatacat acagaaaaag gaattgacta cttagaggaa aaactgaggt
     1441 tggttaaaaa tacagataat gtaaaaagcg ttacgggcta tcttataaaa gcattagaag
     1501 aagattataa gcctatacca agtaagaaca ataaaaataa atttagaaat tttgatgaga
     1561 catttactaa gtatacgtct gatgaactag acgagattat taaaaagagt caaaaagaga
     1621 agtttggagt agggagttaa tataactatt aacttcctag aagttaaaaa aatattggag
     1681 gaatagaaat gataataatt agaagtcaag ataaaactga tttagtaaaa gctgattgga
     1741 taaatgttga taaggaatat gtatatgcaa tgtttggaga aataaataac tttaaggaaa
     1801 taggaaaata tgaggatgaa aaaagagcta tacaagtgtt aaatgaaata cagaagttta
     1861 ttgaaaatgg agtaagaacg gattatatag attcttgcag agttagacat aatcaagaga
     1921 aagtgtttga aatgccagtt gaataaggag ggggaactaa atatggctaa agtttgggta
     1981 gatgcaggag aattgttaga aaaaactatt gatatagaag atatgtttga gcttaattta
     2041 agagcaataa gaaagagaaa tgaaaaaata aaaaatgaaa atggggaaca agcaaaaaat
     2101 gaagagtcag aaatggaaac tacaaatcgt ggtggaccag ggaaacctat aaaagttttc
     2161 aatataaata ctggagaggt taagattctt aagaatgctc aagaagcaag tagatatatg
     2221 aaagttagtt gcaatcatgc tagttattta gctagagaaa ataaatcaac taaggatggt
     2281 tggaaagcag agtatattca aggagtgaca gatggtatta gcaaatgtgg aacaagtaat
     2341 taaattagct gaaaagatat taaataagaa aaagtgttct gttaataaag ctattgatat
     2401 agctataaaa atattgagta aatatgagtg cgaggggatg ttagaaaaat gaaattaaaa
     2461 gatattataa aacttggaga aaagtattgt tattgcccta actgtggtaa tgacaagatt
     2521 ggaaatgatg aaggcaagtt aatagttgaa gaacacacat attatagaga atgttcatgt
     2581 ggattcaata tattaattga tgataggaag gatgaaatat aatgaacatc ttggctagtt
     2641 tacttttgat aggaattagt tttatagctg gtaggattta tgagtataga ttgaatctga
     2701 aagagtgtga aaattgtgat aataaaggag ggtcaaaaat gagtaaggtt atacaatgtg
     2761 atttctgtaa ggaaatattt ggagaaaata atttagaatg tattgagcta tataaaaaga
     2821 atgttgaaaa tgaaatgatt aacatagata agcatatgtg tccagcttgt tatgaaaaat
     2881 tcgttggaga gaaagtagaa aagaaaataa ctaattttga aaaaataact agagataaag
     2941 aaagtttgat aaattttata gttaaatgtg atgcagaatg tgattgttgt atttatgcaa
     3001 atgatgataa ttgttctccg attagttgta ctaaaggatg tgaaaagtgg cttgacatgg
     3061 aggtagagct ataaaaattt ggaatgatga aataagcaaa gaaataaaaa gattaaaagg
     3121 attcttatat acagaaaata tctaattaaa acagtttaga gagttgcaaa atgtctttta
     3181 atataaatta ttgttgaagt gttttgtaac tctcaaaaac gaaaataagg ggtgaacaaa
     3241 tgtcaaagta tgtgcttaga tggcaaatgg gattgttgct agaaaacatt aaaatacatt
     3301 acacatatgg tagcaaagaa atgttaagac aaaaagcaga agtattagct aaagatgaca
     3361 agatattatt tataacaata gataagatag atgaagttat aaaggatact agaagtcaga
     3421 aaatggctga atattttgag gatgaagggg tggaataatg ataatatgtg taatggttaa
     3481 aactaataaa aaaggttcgt atgttcatgg tgaaattaaa gtagatgatg atgatttaaa
     3541 aagtatgagt gatgaagaaa agtcagaaca tatgagaaaa atagctcaag aatatatttg
     3601 taaaaatctt atagattggt actggtttgg aattggagaa aataattaaa aggggtgaaa
     3661 ttatgataat acacaaattt ataatacatg ttttagataa gaatagcgat atgccaatat
     3721 taaatgattt tgaaggtagg gttagtcaag atatagaagc gttcttcaat aagaaaataa
     3781 gtaaagtatc aagagataat gatattagaa cagcagtatt taatgactat agtaacaatt
     3841 taattaagaa gtgttgtgaa cagattattt atgatgaaag ttcattttta aataactcta
     3901 aagagattgc agcttattta tttgatgtta tgaagcttaa tgctacatta gaatcttgtg
     3961 acttagcaat atgtttatat actgaaaaag atgaaaagaa agttgctata ttaaaacttg
     4021 attacaacaa gtcgtatact cattctatta gctttgaaga tgataaattt aatatacaaa
     4081 tgtttaagaa tgaaattaat atacaagaga ctaaagcaat taaaataggt gcaattattg
     4141 gtttgagtgg aatgaatgat gaatatcatc ttagggtttt agacaaggat gcagaaaagg
     4201 aagaagctaa ttctaagttt gttacagagt ttctaaatgc tactaaagtg aaagatgata
     4261 agtataagac gaagaagttt aaagagtgta ttgatagcat gatagcacat atatttaatg
     4321 gagatgtgaa acaagcagag gaaatgagaa ggacagcaat ttacatgcta agagaaaaac
     4381 aaaaacttga tataaatgag tttgctgata aagtgataaa ggatgattta aaagaaagtt
     4441 ttaaggacca tgcagaagaa aagggacttg atgaaagttt taatattgat aaaaaatggg
     4501 ttgagaagaa tttaaaaaat agacatataa aaacagatac aggttttgag ataaaaggca
     4561 agatggatga ttttgaggac ccaatcaaat atacagtaag acaaaatcaa aatgggtata
     4621 tagatataat tattaagaat gtaacatttt atgaagaaaa gtaggtgagt acatgactaa
     4681 taaagaaatg tgcaagtcaa agaatcttga tgaaagagaa gtgtataagg aatttgggaa
     4741 agagatttgt ggtagctgta taaatgataa aagagattgt gagagtaaag attgtgatac
     4801 agcacataaa atatggttgg agaaggaggc tataagctat gttaagcatc tataaagtga
     4861 aattaaagac taaaagaaca ttggaacagg taagaaatca aagtgtagac tttgactatt
     4921 cagaagaagg attaaaaaat gtgcttagat actataactt aattgatggt ttagaagtaa
     4981 ttgtacttaa atttagagat gaatattgtc tagctaattt taatgaagaa gatgaaaaaa
     5041 caataatgga agcacattat cttttagaac aagacgaata tactggatgt tatataaatg
     5101 aatatgaacg atttaaaaga gattgggaaa atggcgactg tgatggggaa ggttatatgg
     5161 tattttcaga tgatgaagtt gagataattg aggaattaag aagtaaatag aataaatagt
     5221 caaggtaagt ttgtgaatga aactagaagg ctatagactt actttgactt ataaaaggag
     5281 tgtgttaaat ggctaatata tattgtgaaa attataattg taaaaattac tttgaagata
     5341 tgtgtatgct tgaaagaatt gaaattaata acttgaaagt gtgtgaaagt tacattgaag
     5401 gtaaaaatga gctatatgaa ttagaaaacg gatatactat gcatcctaaa gatttgaaaa
     5461 tggtgaaaag taaagattat tctgttgaag ttactcatat tccaactggt attacagtaa
     5521 aatgccgttc tacaaatagt attttaaaaa ataaaaataa gtgtttggaa gttctagaag
     5581 aaaaactaat aaaaataaac tctcacttag tgttagaaga tttacgctaa ataggaagtg
     5641 agcttatgat acatgaatta aaaatattac ctcaatattt taaagaagtt gtaaatggga
     5701 acaaaaattt tgaagttagg aagaacgata gaggttttaa aaaaggtgac ttgttgatat
     5761 tgcaagagtt tgatggagaa aaatatacag gtcttgagac acgaaaagaa attacttatg
     5821 tacttgataa cagcgattat atgcaagatg ggtatgttgt tttaggaata aaataaatgt
     5881 tttgtgacta ggaagtgatt ttatgaaacg aagaagatgc agttggtgtg gtaagctatt
     5941 ttatcttaag gaaaaatcta aaaatgttta ttgttgtaag aaatgtgaga gaaaagctaa
     6001 gaaggtgaaa aatgaaaatt aattttgtaa tagatggaga acccgttggc aaggaaagac
     6061 caagaatgaa ctctataact aaaaggacct atacacctaa taaaactaag aattatgagg
     6121 aattaataaa atggctatat caatctaaag ctaaacatta ctttgaaggt tatataaaaa
     6181 tgactttaag atgttactat tctatagcta aaagtaacag caaaaagatt aaagagcaga
     6241 aaagaaataa tgtgttaaga cctagtaaaa agcccgatat agacaatgtg gtcaagatta
     6301 tagctgattc acttaatgag atagcttata aggatgatac acagattgtt gaagttgtag
     6361 ctagtaaata ctatagtgat aatcccaggg ttgaggttat attggaagat attagttgaa
     6421 caacggaaaa atccgtttat caaatcatta gataaaaata atttggagga ggattataag
     6481 tatgaatgat ttaaaattaa tcgaaaatga agggattata aaagtatata caacagatga
     6541 agatataaag gttgtagatg gtagggaact ttgggaagga ttaggtgtta aacaggattt
     6601 ttcagattgg ataaaaacaa atctaaaaag tgttgatgcc attgaaaata aagattattt
     6661 cacaatccct tttaaaaggg agcgtcagat tctaatagag tatactttaa aactagaaat
     6721 agcaaaagaa atttgtttag ttgcaggagc aagcccaaga gcaaataaag aacttaaaag
     6781 aaattctaaa aattatagaa agtatctaat tgcagtagaa gaaaaataca aagcatcaaa
     6841 caatcttaca aaaacacagt taaatcaaat taatgatatt gtaagtaacg cattatatga
     6901 aatgcaaacc aaacatgatg cacaaataga acaatttaaa agggaatcct cacaatatta
     6961 tagacctaca agtaaaacta agtatgatat ttcaagctac ataaaagata gattagacat
     7021 tcctagagct aatgaagagt ttgatttggt taaaaagaga actttattaa ttcttggagc
     7081 tgataagtgg gaggatatac caaaggatgt gttattaaaa tctttaaata ttatagatga
     7141 atcaataaga attataaagt ctgaaagaaa aacaaatcag attagtttct ttgaaaaaga
     7201 taatttctgt taataaaaag aaaaaaggag tgctttcaca ctccacttgt caaaaatata
     7261 aaacttttat atgtaaaatt attataacat aaacaggagt gtggaagtat ggataataat
     7321 atcaataaaa aagaactatt taaaaaagta gaaggtagac tacatcatta taaattttta
     7381 agtgcagaaa ttaagaatct tgaattagat atagaaagta gagaaaatga gatatttggg
     7441 tgtaaggctg ttggatatga cgaaaaagta agtccaacat atgcttttaa ttcaactgtt
     7501 gagaatgaga ttataaaaaa agaaagagat attgctagac taaaaaaact gaaaaaagat
     7561 aaagaaatag aaaagaagaa aatagaaaat gcacttacat gcttagatat aagagaggaa
     7621 cattttttta aactgtttta taatagcaga atgaaaaata gtatggttta tatatcctta
     7681 gagatgaact cagatagaaa aacatgtaga tgtgtaaggg aaagattagt gtataaagtt
     7741 atggatatgc tttatccaaa aattaaggaa aatgagctac cattatttaa aaattagaaa
     7801 attccccagt ttttccccag agattcccac tttattccct actttctccc ctttttgatt
     7861 aaaaaagcat gagataataa tattgtggaa ataaagattt ccctctcaaa acttaataat
     7921 tggctagagt taaggggtta tttttgctct aggcaaagtg aataattttt ataattaagg
     7981 atattatata aacaattaaa aaatttaaaa tgacgtttta aatataaaaa atgcacgtat
     8041 ttgtctttta atattgacac ttttgcacaa aacgcctaca ataaatatat aaacatgtag
     8101 gaggtgcata attatatgcc agaagacgca aaactagaaa tacctaatga aataatctct
     8161 acaattgttc aatgtgttaa tggagctgtt ggagataaaa taaaagaaga tattaaaaag
     8221 caaaatcttg taactaaaaa cagtgtacct acccgtattt gggatttatt aaatagagac
     8281 cttcgtcaag cactcgatag tcctgattgt atggcatatg aggctaaacg aggatgttgg
     8341 gaaatggtca ttatatatga aaaaagtagt gaaaatattt ttactataat gagagaacaa
     8401 cgatttaatg aaatatataa aaaacaacct gaaagaagaa acatgcacta cttgaatgat
     8461 ttgaccaaaa actttaatga agatttaaaa gcacctattg ggcaaatatc ttttttccct
     8521 aaaaaatttg atgatgaaga caaagttaaa gaacatgtac aaaaattact tgagaatcta
     8581 ataaaagaca atgttgttgt taaacaccat gccttaatat tgtttaatgc atctgaatac
     8641 caacttacat ctattcgtgc tgtaatgatt gataccaact tagatattgt tacagaaaaa
     8701 gattggagtt cttatatatc tatagaagaa agtactgttg cagaaaaagt tgataatact
     8761 aatcctgttg caaataatcc tactcttgga ttaaacttaa cttcaaaagc agttgcaaga
     8821 aagaaaacac atcctttaaa aaaggcgaaa aaggaggaag atgccaagta gtcacttttt
     8881 agtgaataga tggaggaaat aattatggaa attaatttta atggagagcg tttaaagaaa
     8941 gctcgtatat atagaggaat gacagtagct gaattagctg agagaattga ctgtcaaaga
     9001 cagactgttt cgatgtatga gaataataaa tcgaaaccaa atgataataa tgtagtgaaa
     9061 cgaatagcaa gagtgttagg ttttccagtt aagttctttt tagaaacagg aactaatatt
     9121 gcaataggtt caacatattt cagagcatta ttgactacaa ataaaaaata tagagcagag
     9181 caaattcaaa aaatggaatt tctagctgaa atatatttct tcttacaaga ttatattgaa
     9241 tttccaacat tagatttacc agattgttct ggtaaaacac cagaagaagc tgcgttactt
     9301 ttaagagaag cttggggatt aggattaaaa cctattgata atatcatata tgaagtagaa
     9361 caacacggaa taattgtaac aagcttttca acttctacag atgatataga tgcatttagt
     9421 cagatgatag atatttcagg agaaactgtc tatattattg gatattctag caataagact
     9481 tctgcttcta gaattcattt tgatatagct catgagttag ggcatatatg tttacatgaa
     9541 tggagtgaag atgtagaagc tttagaaaaa caagagttta aagaaagaga atcagaagct
     9601 aatcgctttg catctgcatt tttattacca gaagaaactt ttaaaattga tgctaagaga
     9661 actcctttac gtatctcaaa ctatacagaa ttgaaacgta aatggaaagt ttctatacaa
     9721 gcaatgattc gtcgttcata ttctttagga atcatcagta tggatgaata tcagtctatg
     9781 attcgtactt tacaacgtag gggactaaga aaatcagaac cattagatga tgaattatta
     9841 acttctctac cagcattgtt aaaaacagca gttttgatgt tattaaatga gaaggtattt
     9901 actccaaaag agtttatgga tgaactttca ttttcctata actttagtct agagccagaa
     9961 gaagtagaat atttattaag tttacctaaa gatactttaa cttctgctaa agttatacca
    10021 tttcctgact tacaacttaa aaaaggtatt taacttatat tttaaataga gcgaggacat
    10081 aagtcttcac ttgaattata tagaataggg ggaaatatga aatgggaata ttagaagatg
    10141 caacaaaaat aagagagata gctaaaagaa ttgctagaga aaagggaata acagaacaag
    10201 aggcatggaa tgatgctatt gaagagtata agggaaaata tgaatttaat taagaccagt
    10261 atatggtctt ttttttatta ctatgaacag actaggcagg gcgtgaggac gctgttagtt
    10321 caattctaac tatgttcaat cttcaagact tttctcttga agttttaatc tacagataca
    10381 ctaaaaaata gtatttgaat tgagattaaa atctcataca gttttgtatc ttaattcaga
    10441 agtctaaaaa ccgagtgggg cttggtaacc tcactcacca tgcaagtgct ggtgtttaat
    10501 ctaagttcga ttcttagaac ttgcgacata atatatgcat ctccctgcta aaaaaggctt
    10561 agattaattt ctaagtcttt tttaatacaa aaaattaata agtttcagat gtcggtaact
    10621 ccgacagtag agaaggctta gattaatttc taggtctttt ttatacaatt ttaatccctc
    10681 atattgaggg ggcgaaaata agggtattca aattgaacac cttttattaa taactaattt
    10741 aggaggtagt agtatgttga aaattttaca agataaaaat gtaaaagtaa tgtggtccaa
    10801 aaatggagaa gaagtttggt ttaatgcaaa tgacgtagga gaggaactag gcatagtaaa
    10861 tattcgtgat acattaagaa atatagatag agaatataaa aagaaattta atgagtctac
    10921 tgtcggagat tcctacacta gaaactttaa agataaatta cctaacttcg gtactacttt
    10981 tgttacagaa gaagctgtgt acaatatgtc atttagaagt aataaaccag aagcaaagtt
    11041 atttacaaaa tgggttacac aaacacttaa acaaattaga atacatggtt actacattgc
    11101 tacagaaaaa gaccaagaat ggttggatat aagaacagaa ggtaaaaaag taagaaaaga
    11161 ttttacagat gaaatacaag agtttgttta ttatgcaatt agtcaaggaa gtaataaacc
    11221 tcaaatgtat tataaacatt ttaccgaact tgtaagaaga aaattaggta taccaaaagg
    11281 tgtgaaaagg gatgagttaa atcaaagtga actgtttgac atacaagcac ttgaaagaat
    11341 tatatctatg aaattgccta agttgataga caaagatatg aattacaaag aggtatataa
    11401 aaagattaag gaattaatag aaatgattta aatgactatc ttgagggatg gtcttttttt
    11461 atacaataaa ttaaaagaag aatggtaaat gtgttatggg attaaggttt tgaacttatg
    11521 aaacagagta acagaactta gtaggaatta gctttattct ttattatgtg gtataatata
    11581 aaatagagat aaggaagtgg ttagtatagg aatattaaaa agtgctgaat tagttagaaa
    11641 gatagcacaa aggattgcta gagaaaaagg aataagagag caagaagcat gaaatgatgc
    11701 tgttacagag tataaacaaa aacatagata cttagtctag agaatcatct ttatagatgg
    11761 tcttttttta tgtaagaagt ttgtgtaata aattaaaagt aaatgtaaaa aatattatac
    11821 aaatataata aaataaggta atgtttttaa ggagaaaaag atatgctcaa tcagtttaat
    11881 gaatttcttg gtaactatat aaatataatt tctgtaattg catcatttgc atcaatagca
    11941 gccttgatta tatcaattat agcttttatg gaatctaata aacaagctaa attgatatta
    12001 aaatatagaa gaaataatga ggataatatg gctggtgatt attatgaaaa tggcaatgga
    12061 tatattaggc ttgatttaaa taagatagat gataaatcta atgtttatgc tgaaattcgt
    12121 agtaataatt tagttgattt tgtaatagaa aacaaaagta agattgttgc aaaaagtcct
    12181 atattaagtt tgaaatttat taacatggaa gtagatattg aagaaaataa taactttgaa
    12241 caaataaata gtgaacttag atggcatcca aaagataata ctacaattca taaaggaata
    12301 aattttagaa tagagcaatt taattttaaa aattgtgtta tgcttaaaaa agaagcttat
    12361 atagaagtag tattatcagc tgataatatg gaaacaaaag aatttagtat accaataaaa
    12421 gcgtgttaaa gaactctaat cagagttctt tttttatccc caaaacaaac aaaaacgagg
    12481 tggtgatgtg caagatgtca aagaaaaggt aaaaaaggat tacctaaaag gtatgaaaca
    12541 taaggaaata tcagcaaagt atgatattag tttaaatact ttaaagtcat ggataaaaag
    12601 atacaactgg tcaaaggaaa aaaagaaggg tgcacctata aataaaaggg gtgcaccctt
    12661 ttctaataaa aattcagttg gatatggtgc tccaaaagag aataagaacg ctgaaaagtt
    12721 tggcttcttc tcaaaatatc tacctgaaga aactagggaa ttgatacaag aaatatctat
    12781 aaaagataaa ttcgatattc tttgggaaca gataacaatt caatatgcag caataataag
    12841 agcacaaaag ataatgtatg ttaaagataa agaggaaatg attaaggaat taaagaaaca
    12901 tgaaagcaca gaaaatagtg agaagataga gtatgaattt caatttgcat gggataggca
    12961 agcatctttt cttaatgcac agagtagggc tatgagtgaa cttagaagtt taattaaaca
    13021 gtatgatgaa atgattcata aggattggaa tttggctaca gaggagcaga aaacaagagt
    13081 tgagaagttg aaaagtgaag ttgataacct aagtaaagat gatacagata aagagataac
    13141 tgtaagagtt atgaaagcga gtggagaaaa tggaaagtaa atttatatca aataaccact
    13201 tttatgattt tatattcaat tgggattaca aattttattt tcttgttgga ggatatggaa
    13261 gctcaaagag ttatcatgtt gcatctaaat taattattaa gcttcttaaa gaaaaaagga
    13321 aagctttagt ggtaagagaa gtatatgaca caatgagaga tagttgtttt tctctattag
    13381 aagaagttgc agaaagtatg ggtttaggag atgtattatc atttaaaaca agtcctatga
    13441 gagttatatt tccaaatgga agtaagataa tttttaaagg tatggataat ccagccaaat
    13501 taaaatcaat aaatggtgta tctataatat ggttagaaga atgttcagaa gttaaataca
    13561 caggttttaa agaactctta ggacgtttga gacatcctag tctatctaat catataattt
    13621 gttcaactaa tccaattggc gaagataact ggacttataa acattttttt agagatgaag
    13681 aaaaagacag atttgtatta gatgatactg tgttatataa aaatagaata gtaacaaaga
    13741 ataataccta ttatcatcat tcagttgcag atgataattt ttttctgcca aaaagctata
    13801 ttgaacagct tgatgaaatg aaagaatatg actacgacct atacagaatc gcaagaaaag
    13861 gaagatttgg tgtaaatgga actaaagtat tgccacaatt tgaagttatg gaacatcaag
    13921 aagttataaa taaaatatct aatataccac aaaggtatta taaagttgga ttcgattttg
    13981 gatttgaaaa atcatacaat gcacttttaa gattagctat agaccatgaa aataaatatt
    14041 tatatattta ctgggaatat tataagaacc aaactacaga tgatgtgact gttaaagata
    14101 ttaaagaatt taaagacact caagaactaa taaaagctga tggcgctgaa cctaaaacta
    14161 taaaatattt tagacaacaa ggttttaata tatctggtgc taaaaaatat gctggaagta
    14221 gactacaaaa cactaagaag gttaagagat ttaaaaagat ttattgttca agtgagtgta
    14281 aacatacaat aaaagagtta aagaacctaa catacgctac agataaaaat ggaaacttaa
    14341 tatatgatga atttaatata gaccctcata catttagtgc tatatggtat gcacttgatg
    14401 gttatgaagt gtcagatatt aaggagttaa aatataataa tgatatttac aataagggct
    14461 taggattgaa gaaaaataat ataccaaatc aatataataa gaaaggaggg gctgtatttt
    14521 agtgagtata aaaaatatct tgcttaattt ggatgaaaaa gaattaaaag atagaaaaca
    14581 tgcagaaaga gatttcttgt tttatttagg tgaatgtaga aataaaacaa tgggattatt
    14641 agatgatgat tttctaggtc aaagttggat tacttttgat aatttagact atacaccttc
    14701 tcagattata gataataagg taaaaccact tataaataaa caagctcgtt ttatgtttgg
    14761 aaaagaacca actataatac ttaaagcata tgaaaaagaa cataaagaat catgtgagga
    14821 attaaggcaa tatattgatt caatattaaa tgccagcaag ttttggagca acacactaaa
    14881 agcttttaag atagcaacta taacaaaaag agttttatta aggttagaag ctgaaccaaa
    14941 tcaacctata aggctatttt atcattctat agatgatttc aaatatcaag ttgatagtaa
    15001 tgacattaca aaattaaaat ctgttatatt tgttagattt gatgcttcaa ccataaaaga
    15061 agtaacagcg aaccaaatat ggtatagata tacttattac atgaaggaaa gtaatgttaa
    15121 taaccaagaa agttgttttt taagagtaga aaaatttaaa ggtgataatt tatctaaacc
    15181 tattgaagta aaagaaagtg ataccaagct ttctaaaata ccatgttggg taattgttaa
    15241 tgaacaaagt attacaaata tcacaggtat tagtgatatt gaggatttaa agccattaca
    15301 agatacttat aataaaagat tatcagattt caatgactct ttaaaatttc tgatgtttgg
    15361 gcaaactgtt gtaatagatg cgacagaaga aacagttaat gcttgtaaga ttgctcctaa
    15421 tgctttaatg gcattaaaaa cacttgaaga aggttcagaa aaaggaaaac aagcacaagc
    15481 gtatagggtt gagagtagtt tttcaaatgc agaccctgta aactcttttt ttaaaagact
    15541 tgaagatagt atgtatgaaa aattagcgat acctagacct gaacaattac agaatatacc
    15601 tagtgctaaa gctttgaaat atttatatac agagcttatt gcaaggtgtt cagaaaagtg
    15661 gaatgattgg gaacctgcaa taagaagtat gttaagacta atcgttgaag catgtagtaa
    15721 atttaattgt tatgatgatt ggaatcatga ttgggatgat ttaatgtttt ctattgtatt
    15781 aaataaaaat tatcctattc cagaagatga agaagattca aagagattgg cgcttgaaga
    15841 agtgaacaat aatgttagaa gtcatagaaa ttatataaaa gaatttggtg atgatgaaga
    15901 ttatgaagaa gcatttaatg aagtattgga agataatgaa aagatacaat cagtagagca
    15961 agaccagttc agaaaagacg cagaaataga aattgataat attgatgaag aattaaacaa
    16021 taaatctgat aatagtggca agtaatgagg tgttgttatg aaggacaatg cttatacaaa
    16081 aaaggtttta gaggctagaa agaaactttt attattagat aaaaaagcgc aactagagat
    16141 attaaatgta tataaagatg ctagtaaaat cattttaaat gatattgtta agaataaaga
    16201 attaaatcta agtagcaaat acctaaaaaa gctaaataaa tcaatcgaga agtacattaa
    16261 cgagttaaat caaagattag tacctgttgc agaaaaaagt ataacagaag cttctaatat
    16321 agctaaagat ttacaaatgt attactatca atcaatagtt ccaaataaat ctataaatct
    16381 tgcatgtgat gctatgtgta taaagacaac tactaatgtt gtggaaaaaa tagttacagg
    16441 taacttttat aaagataaaa ggtcattaga tagtaggatt tggggatata gtaataaaaa
    16501 tagaaaagat attgatagat taataaaagc taatgctgct agaggtgcta atgcaaaaac
    16561 tttagcaaag agtttagata attatgttaa tccagttaga aaaacagagg ctaaaacatt
    16621 agaagttgga atgaataaaa gtatatctta tcaagctcaa agacttgcta gaacatctat
    16681 aacacatgct tttgtggaaa caagtgttca aaatgcaata aataatcctt tctgtgttgg
    16741 tctacaatgg aacctaagct ctcaacatta tattagacaa gtgaaatgga ggggagagga
    16801 tgaatgtgac gagtatgcag agcaaaatag atatggatta ggtgagggag ttttcccgtc
    16861 cgaaaaatat cctattccac accctaattg tctttgttat cccactcaag ttatagtgcc
    16921 aataaatgaa gcttcaaaat tcatgaatga ctggttaaat ggcatggata atagcaattt
    16981 agatagttgg tatgatgaaa ttaacacaga aactactaat ctatatatac ctaagataaa
    17041 atattcatct aatgatgtga aaagtaagta tggaaaaact ttaaataaaa agattaatag
    17101 ttttgaaagt aaaatagaaa atacatctag caaatataaa aaatatacaa ctggtgttaa
    17161 gtatgaggta acaaatgagt taaaaactgc ttttgcatac tcaataaaag atgatataat
    17221 taagataaat cctttaagcc ctatatttaa agagtataat tttgaattgt caatgatgca
    17281 cgaattaggc catagagttg atttaaaaga acttgaaata tataaaaata ttgaatttaa
    17341 agatgctatt ttaaaatcat ccaattatgt tttaaataat cttaaagaaa tacaaattga
    17401 atatgattat ttaataaatc taaatgatag ccctttttta agtgatatat taggagcatt
    17461 atcatttaat aaaatcgagg ggcttttagc aactcataat ataacctatt ggcaaaagca
    17521 aggtaatata gaaaaagaaa tatttgctaa tttatttgct ttagattatg aaagtgacaa
    17581 acatatatta agctttataa agaaaaatat acctgacata tataaggtat tcaataaaat
    17641 tatttaggag gtgtgcattg tgggcgaaca aatgccaata atgaaaaggc ttagaaatga
    17701 taaagaacta ttggaactaa gaagattata taaagaaaaa tacaatgaaa atgctcccgg
    17761 atttaattat gatgaatatt ctagttatga tgagtacaaa gaaaagctaa aagaattgat
    17821 acaaaagtag agtaagcact tattaattaa aggttaatga gtgcttttat tatgcttaat
    17881 tttaaggagg aaattgaatg ttagagtatt ttaaaaagtt acttggagat gaagaaggac
    17941 aaaaagttta tgaaaaatta tctaaagata aagaaaataa gcttctttta gataatatta
    18001 aaagtcctag atacgttgaa aagacagaac tagaaaatgc taataaagag attaaagagt
    18061 ataaaaagca aataggggat agagataagc aactaaatga tttacaaggc aaagttaaag
    18121 ataacaaaga gttatcagat gaaattgaaa gtcttaaaaa tgcaaataaa gaaattaaag
    18181 aaaatgcaga aaaagaaatt gaggttttaa agtttaatac agcttttgaa agagcgattg
    18241 aaagttataa ccctcgaaat ccaaaagctt tatcagcatt aataaataag gaaaatatca
    18301 actttgtaga tggcaaaatt attggattag atgaacagat aaaagcttat caacaaagtg
    18361 attcttattt atttaataat gaaaaaaata aaggagatga tgaaaatgta ggaactggag
    18421 gactagaagg aggaacaaca tcattacttg ataatggcag cgaaataagt agtataggtg
    18481 agttgctagc taactctaaa gtaaaaagtg aaaatataga agctcaaaag aaattctttg
    18541 gagaagaata ggaggaaaat atatgagtat agaaaaatct gagatttaca tgggggaaaa
    18601 taaaaccata ttaaaatttg caggaaattt atttcaaaat gtaaatatta aagtgaaaaa
    18661 aactgatgta gctgtagtag atggaaaaag aattttaaaa gcaggtacat taatatcaaa
    18721 agatggaaaa ttagtagatg gtacaactgt tacaaatgat aaagcttttg gcctagtata
    18781 tagaaatata gattttactc attcaaatgg taatgaaagt attccagtac taatatttgg
    18841 atttgtggat gaaaagacct taccatcagt tataccagaa gaagcaaaac aagcaatgaa
    18901 aatgattatg tttttataaa ataaatatta ggaggaatta aaatggattg gaaagacttt
    18961 atagactcta aggagatagc taaatatata aaaaagttac cactagaaat gttaataggc
    19021 gaatctttgt ttcctagaaa aaagcaaata ggtatggacc taaaatatat taagggagca
    19081 aagaaaaaac cagtagtgtt aaaacaaagt acttttgatg ttgctgtaaa aataagagca
    19141 ttaaaagctc aaatagaagt taaatcaaag agaatgccat tttttaaaga gagtgttctt
    19201 gtaaatgaag aagatagaca acagttactt ttagcttcta aagctcaaaa caaagaacta
    19261 ttattaatga taatttcaca aatatatgat aattatttag cccttgtaga tgggggcgat
    19321 atgcaaatgg aaagaatgag aatgcaagct ctagctgatg gagtaataaa tatagtatca
    19381 gaagatggag atttagtatt tgactttgaa gttccaagta atcataaaga agtgttaact
    19441 ggaagtgcaa catgggataa tccagatgca gatattatag gagatattca aagatggatg
    19501 agaataatga gagatgaagg gaagccgtta cctaagcgaa tggtaatgac aagtaaaacc
    19561 tttgggtatt ttgctaaaaa caaagctatt aaattggata tagacaaaga tggtagagtt
    19621 atcttaactg atgaaatgat taaaaattat cttaaaaata aagttggttt atctgttgcc
    19681 atagtaagtg gaacatataa attagaagat gaaagtgagg aatcttattt ccctgacaac
    19741 aaaataactt ttattccaga cggagattta ggaaaaactt attatggcac aacaccagaa
    19801 gaagctgaca aagtatatgg ttctaaatta gattgttctg ttgttagaac tggtattgct
    19861 ataacaacaa tgagattaat tgacccagta acagttcaaa ctaaggtatc gcaattaggt
    19921 atgccaagct ttgaacgtgc tgatgaatgt ttctttgcta cagtagcata attaaggggt
    19981 gataattatg gctaaaaaga aagataattt gcaagtaaaa gctttggttt acttaaaata
    20041 tgataatgac tgttataaaa tagatgatgt gtttgagatt agaaaaattg accaagaact
    20101 catggaagaa aagggctata tagaagtaat aggagaagtt gaggaaaaga gtaataatga
    20161 gattctcaac aaagatggtg agtagacatg gctattaaca atttagataa attaaaactc
    20221 aatctacaag aagaagagta tccttatttt gcagatgaac aacttgtggt gttattagaa
    20281 tctaatgaga ataatgtttt aaaagcttca tggagaggtt gtttactcaa aggtgctaca
    20341 gatgattgta ttaaaatagg tcctatagaa actaaaagta gtaatagttc gtactggttg
    20401 tcattagcag agatatataa aactgattat ttagaagaaa aatcaaagaa tgaaacaact
    20461 aacacaggat ataaaacatc tatgacaaga gttgatggtc aatgagaaaa ctaagagctg
    20521 ataagataat aaaaactgtt aatcgaggga tagctttaaa tcctcaaaca ataactatag
    20581 agcaggaaat taaacaaata gtggatggag ctattgagat tacaaatgaa ataaaagaac
    20641 taacagtagt tatatatccc gaaaaaacta atgatacagt aataaatagt gaaactattg
    20701 gtacagctta taaaaataaa aactttggta tggttgcaga taaagaagct ggtttaagat
    20761 taaatactga aagcgatata acatttaaat gtattgaagg tactatgaaa ctaaactatg
    20821 taaatcctat tgtagttgag gaacaggttt gtggttatat atgtggtctt gaaaagctag
    20881 attagaggtg atttattatg agtgtgttta ctaaggctat aaatgaaatt gataggaaaa
    20941 aggctacaat gccacttttg tgtatgaata tagcttttat gttagaagga gaagctaaaa
    21001 atagtgcaaa atggactgat aggacaggga atgcaagaca aggcataaca ggaactagtc
    21061 taggtggagg aaatcaatac attgttagat tagggcatgg agttgactat ggaactgttt
    21121 tagaggaagg ttcagcacct catgttataa aaccaagaaa tgctaaagct ttattttgga
    21181 atggagcttc acatcctgtt atgaaggtcc aacatcatgg aactaaaggc actcattctt
    21241 taaaaactac ggtcagcagg aatatgccta agataggtaa attaatagaa gggcattgga
    21301 gtaaataata tgagagcagg aataagaaaa accttaattg ataatatact aaggcttaaa
    21361 gattgttatg aaccaactgt acctaataaa aagactataa aaccatatgc tgtaattgtt
    21421 caaggcgaag attctgacag cgagggcgag gttataggct ttagaagaac cataaatatt
    21481 tggatatatg agaaaagaac cacatttaat aaattagatg aacttacaaa agaagttata
    21541 gaaatattag attttaaaac tataacagat gatactacta acgaggtatt tacatgtatt
    21601 tatgaaggtg cagttggtca agatgtcata gatgaagaat gggaagctat aataaggtgt
    21661 ctgaggttta gcataatagc tttagatgat aaagaagata taactaatga tagatgggtt
    21721 gaatctttgt caaagtacac aaaagatttg ttggaaattg aagtttataa ggataattgg
    21781 aagaaaaatt ttatagcacc atgttgcttg tggcgaactg cgaatgtcga aagtaaaagg
    21841 attaactatc atctaatcga gattactaaa actatgaagt gtcatgttgt aagtaaaaat
    21901 aaagatgaaa tagttaagct tcttgaagaa ttagagacta agttaataat agataaaaga
    21961 gtaagactca gagaagataa aaacatgtat ttaactcttg taagtgtagt tgaagataga
    22021 gagtcagaca tgttttctaa tggtcaatta actgtagtat ttaaaatgat aggtaagata
    22081 aaaagagaag gacctattat gaatgagatt tataatagcg gaaacttaag ataggaggtg
    22141 cgagagttgg ctgaaacaat taataaaaag actaatataa gtaagcagga agaaaaatat
    22201 tcaaaagaag atttcttaaa aaatagtgaa gcacttggct acaaaaaaga agtagtggca
    22261 ggtgctttat ttaattgtaa gaaaaaagaa cttacaaaag tagagtttga gaaattaata
    22321 aaagatttct tagaaagaga ggtgaagtag tatatggcaa ctggaacatg gaatgagaaa
    22381 gaaaaaaaag agatacctgg tttttataac agatttaaga ctcaagcaga aaaatctaca
    22441 aacacaggtt taaagggtag attagcaatg ccagttaaag ctaattgggg agatgtaggt
    22501 aaggtaataa caataaaaaa cgacttaaaa cagcttaaag ctctatttgg tgatgatata
    22561 agttattcag cttataaatt aggaaaacta gctttactag ggaatgtaaa ggaactattg
    22621 ttgtataggc tcgtagatgg aaatcaaaaa aatggtacat taatacttaa agatactaca
    22681 gaaaatactg caaaagatgt aattaagcta gaaactaagt atccaacagc tagaaacttt
    22741 aatgtaacaa taaaatctaa tttagtagat tcagataaaa aggattttct tttctttgaa
    22801 ggaactaaac agttatttag ttcaagtatt aaagggacta tagatgaaat agtactagaa
    22861 ataaactcta atttagataa tgagtatata gttgcaacta aagtagctga tagtgataca
    22921 acattagcaa acttagtaaa tgtagcactc gagggtggaa atgatggttg cacatctata
    22981 acaaatgaat cttatttaaa agctttggag gagtttgaaa gatatagttt tgacggtttc
    23041 tcattagatg gtgtagctga ggaagcttta caagaaacaa caaaagcttg ggtagctaag
    23101 aataaagaat taggaaaaga tatattatta tttttaggtg ggaaaaaaga ggataatata
    23161 aaacagatta atgataaatc aaaaagtttt aatgatgaaa atgtaattaa tgttggaagc
    23221 tcagtttatt atgagggaat aaaatataca cctagtgaag tagctgttta tataggagca
    23281 ttagcagtaa gcaaaggtat aacaggtagt atatgtaatg ctaagactat atttgaagaa
    23341 gtagaaccaa gactaagcca atcagaagtt aaagagtgtt taaagtcggg aacattaata
    23401 cttgattttg atgacggaga cgtgattata gttgatgatg taaacacatt taagaagtat
    23461 acagatgata agaatgaagc aatgggatat atatctaata ttatgtttat taacactata
    23521 aataaagata cttcattgaa aagaaaagag tttgtaggga aaatatttaa tgatgcgaca
    23581 gggcagacaa ctgttatatg tgcattaaag aaatattttg aagaattgat gtcacagggc
    23641 atcatatcag aatttaatgt tgatatagat acagaacttc aagcaactgc caaagcagat
    23701 gagttttatt ggaaatggga tgctattaag gttgatgtga tgaaaaaaat atatggtact
    23761 ggttatttag gataaaggag gtgtaaaaga tgagcaaata taatgatgat tatatagaag
    23821 aagcaaattt tttgaatggt tctaatgtaa gaataattat agatggcgaa gaagaaatgt
    23881 atgcagaaga aataaaagca gactatgaac aagatgagca aagtgtaaag ttattagggt
    23941 gtgaagacga gatttcaaga ggtggaacaa aaaaaggttc tttttcttta actagattta
    24001 aggttaattc aaatatatta aaacttggtt ttaaaacttt tgagataatt tacgaattaa
    24061 gaaatgctga aacttttggt tatgaaagag taaggttaaa aaactgtaga cttaaaaaat
    24121 acctgtcata aattcaaaag caggtgaaga tgtaaaagaa gagttagaag gttcattcag
    24181 aggatatgaa ttattagatg cactttaaaa taatactaca cataaagcta taaacaatta
    24241 atttgtttgt agctttttta ttaaaataaa aaatattgga ggaaataata tgttagatat
    24301 ggataaaaga gagttagaaa atgaggaaat agaaaaagaa gattatgatt tagatgaaga
    24361 aatagaagaa acaaacgaag ataggttgat gatgaaagag gatgaaataa ttgcaaggtt
    24421 attagaggat actccgatac ccaaaaggac tgtattttta gagagactag gtattccaat
    24481 tacattaaag gcattaacag aaaaagagat tagcaaaata agaaaagatt gtactaaaat
    24541 ttcaaaggtt caagggcgaa gggaagaaaa attaaatgat gatgaattta atcttgcatt
    24601 aatagaaaaa ggtactctag tacccaattt ctctaataaa aagttgcttg atgctatgaa
    24661 agtatcagat gcaaaagaat atataaaaag gaaattctta gcaggagaat taagtaaaat
    24721 aagtgaccaa atattagagt tatccggatt ttatgatgaa ataagtgacg atgatataaa
    24781 aaactaataa aaatgggggg acgactgact gttttaaata acatctttgt taagcatcat
    24841 gttcccccag atatttatgt caagaaaaat ttaatgtctc aacacttaat gaaagtgttt
    24901 actcaaaatg aaatagagca agagaataaa gctatgaaaa aataaatatc tgtagaaagg
    24961 taggtgaggg aaatagctaa aaaggaaatg tatcatattg atgttgtcat aagtgcaaaa
    25021 ggtgatggag aaacaaaaag caagctaagt gcaatggaaa aatacacgaa gcaaactgaa
    25081 aaaaggatgc aagcacttaa tagaataaaa gttaatcctg ctataaaagc tacagataaa
    25141 gcttcaagtg ttgtaaatag agttaataac aatatgaata aggcaaaaaa aactgttaca
    25201 gcaagaataa aggctacaga taatgcgagt tcagtggcta atagggctag caataatgtt
    25261 aataaagcta gaaagacagt aacagcaagg ttgaaagcca cagataatgc gagttctaca
    25321 gttaacaaag ttaataacaa aataaaagaa gttgctaagc ctgtacctcc aataatcata
    25381 cgaggacaag atgagtctag ctctataata gataaagtaa aagctaagat tcagaatcta
    25441 aaagctgata ctattataaa aataaagtct caagctgatg aagctataaa tactatttct
    25501 cgtactaaaa ataaattaca agaatttgtg agtaagagat atgaagcagc agtcaaaatt
    25561 cgagatgaag ctagttcagc acttggagga attacaggga aaatagactc ttttgtaagt
    25621 ggagctatta gtaaatttgc tcaattggct actatggcag gaactttaat aggtggaatt
    25681 ggggtaggtt ctgctgtaaa aggatttgct acttttgagc aaagtatgaa gaatacacaa
    25741 gctgtaagtg gagcaacagg aaaggaaatg gaagctttaa ctgcaaaagc tagacaactt
    25801 gggagagaaa ctagttttac agctaaagat gcaggggatg caatgtatta tatgggtata
    25861 taacatgctc ggctataaag aaatttatag taaaaaatag tgggttaaaa taggaaagct
    25921 aagtataaat ttatatatgc taatcaatta ccaattttat tagggataat aaaaaggttt
    25981 agagactaga taaagtaagc tagaacagtt gaaatatcca cgaaatccac taccctaacg
    26041 taccaggcga gggtaaagag atagtccaac tctaagggaa accttagttc taagataaag
    26101 agcttagata ataaagtaag atggcaggat ggaagtccga gcaaatgata aaagcaattc
    26161 ctgacgttct taatttggca gcagcaggag gaacagactt agcactaacg agtgacattg
    26221 tgactgatgg actaacagcg ttaggaatga ctgcaaatga cacaactgaa tttgttgatg
    26281 tgatggcagc aactataaca aattcaaata caagtgttga gttgatgggt gaatggattg
    26341 cccatgcaaa agctatttaa ttcggtgaac cctaagttga ttttttaata cgggaatacc
    26401 gagcgaagct agttcggaaa cgactagaac gtgtaacgac tagacaaagt atactaagta
    26461 aaagtatttt tatatgttga aatgtccacg aacagtagct atcgaattta aaaatttggt
    26521 aaagatatag tctgaactat atagaaatat atagaggtaa aggataaaga gcctttacga
    26581 taacaaaatt ggagacattc aaatatgtag gttctatggg aggagcatta ggagtatcca
    26641 tgaaagacct ttcattagct acagggctaa tggcgagtgc atctgttaaa ggtagataag
    26701 actgccgttc taaatagtga tgtttagaat tataatcggg taaaatcggt gaaggctaag
    26761 tttaatttaa ttaaatatgt taataccgag ataactataa acttaaaatt tttatagtat
    26821 tgtagagcat agagattgaa actagaaata gaatataata tctccaagag tatccgacta
    26881 cttttttaaa gtagaaaatg tatgccgaac ttataggaaa ctataagaac tagaggataa
    26941 aaagcttcta ggataacaaa tttgagtatg gcaggtactt cgttaagagg aggtctagtt
    27001 agattaataa agccaccaga agaagccgca tctgcaataa agaagtatgg aatagaatta
    27061 aagaaaaata aaaatggaag tttggattta gcaggaacaa tagtaagttt aagagaaaaa
    27121 cttggcggat tagaagatgt tgaaaaaggt gttgcaatat cttctatttt tggaagaaca
    27181 gccatggcag gttgggcggc tgtagtaaat gctagtgaaa gtgattttaa taagttaact
    27241 acggctattg cagaatcaga aggtgaagct aagagaattg ctgatatgaa actagatacc
    27301 ctgtcaggac aatttgaaat tttaaaaagt gctattgatg atgtaagaat atcggtagga
    27361 agtaaactag gtcctatgac tcgtcaattt gtagaaaaac ttataaaaga tatgcctaaa
    27421 ataggtgatg ctatagttgg agtagtagaa aagtttgtta ataatttcga taaaataaaa
    27481 gcaggatttc aagtattatt gcctgcaatt ggttccgtta tagtctctgt tatggtactt
    27541 aaagccacat ttgcttttgg aggagctatt aaaaatttaa gtttacttgc aagtaccttt
    27601 ggtacggcta aattagctgc ctttggatta acagttggca taggggctat tgttatagct
    27661 tttgcaggaa tgactgttgc tatttctaat aataaaactg ctatgatgga cttacaaaca
    27721 cgttttggtt cgtttggtga atatgtaact acaataatgg aaactgtagg tggagttata
    27781 aagcttacgc tagggaatct acttataatg cttggaggga taggcaaagg aataggtatt
    27841 cttttatcag ataagagctg ggatgaaaaa gcttcttcat taaaaaatct atttggtaaa
    27901 acaacagcag agattaaaac aaatacaaaa gaagctttga gtgatataaa tggagaaact
    27961 tctaatgcaa cagctttatt aaaaaaatct acttcaaaag agttacaagg tgttactaaa
    28021 gctttctcaa cagcttttga ccaatctaaa aatgtaacag agaagaaatc tagtgatata
    28081 gctagagcat taacgaatgc attaaaaggc ttagatgacc aatctcttgc tatgatgcga
    28141 ggattaaatg ataatatggc gataatttta tctggtgtga cagcagatat gaaacctagt
    28201 gacaaagtta acaaaatcac caaaaatctt gatgatgctt tcaaggcggg taaattaagc
    28261 gcccaagaat atagaaatag tatacaagaa actttaagtt ttataagtaa atatagcaca
    28321 gattcttcaa ataatttaaa acaaggcatg agtgacgctt tcaatgcatt taaagaaggg
    28381 actaatatag gtggtctaaa agacggagta acagggatgc taaattcgct gaaatcaaca
    28441 ggaccacaag ttctagaaac attaaaaagc ttgggaggaa aagcaagcga gatttttaag
    28501 ggagtggatt ttaattcttc tatagacacg caaaaaaata aagttttaga aaatctaaat
    28561 agtttaggat tagaaggtac acaagctata aatacattaa gaactatttt ctcacaagct
    28621 tctactgcac tagatactac aaacttaaaa aaaggcttga gtgatacatt taattcattt
    28681 aaaattggtt ttaaccaaga cggtatgaat ggagcaataa gcagtatgct tggtaccatt
    28741 aatcaaatag gaccacagat gcaagcggct ctagggaaaa tggatggaaa tatgggtcaa
    28801 gtatttgcta atgtagattt tagcactccg atagaaacac aagcttctaa agttcttgaa
    28861 aatttgaata atttaggaat agaaggacca aaagctttag aaactgtaag aagtatattt
    28921 actcaagcat cttcacaaat acaaggttca gcttctcaaa ctgcacagca agcgaatcaa
    28981 gaagtggcta atgctctaac acaagaaaac ccagctatac aacaggcagg gcaacaatta
    29041 ggtacagatt tgacaaatgg tgtagtaaat ggagtacagg caggagtgcc agttgtacag
    29101 caaaaaagta atgaactggc tactgctaca caaaatggag taacaaatgc tgtaaatagt
    29161 gcaactcctc aaattgataa tagcaatctt acaaatggta tagacacagc attcaaccaa
    29221 gctactgcaa ctgttcaaca aggagcaacc aacatgtata acggggctaa acaaagcttt
    29281 agccaattag ctcaagttgg aagagaagca ggaagtagtt tatataatgg tgctacaact
    29341 tcttttaata tgttagcaac aaatgttaga gtagcatgta gtagtatgta caatggtgct
    29401 agaacatctt ttacaagctt aagcagttca gcaataagtg ccatttctgc tatgtgcagt
    29461 tctgtagtaa gtcaagttag tgctatgtcg agtcaaatta ttagttactg gaatagtgta
    29521 agagcaacag tttcagcacc aatctctgca tcttttaatg ttaagactac tcaaacaact
    29581 gtcaaaagga cagtaaatga aagtagtggt ggaatactaa gtaatatatt tggtcgattt
    29641 gctgatggtg gagttgcaag taaaccaagt atttgtggag aagatggagc tgaaatggtc
    29701 attcctttat caaatgctag aagaaatcga gcgataggtt tgtatgaaca gacaggcaaa
    29761 atgttaggtt taagctcaac tccatcacaa aaaattggag gaagctattc caatgttgta
    29821 aataatgtta gacaatttcc tttcacaaat gttttagata cagataataa agaatataaa
    29881 gaagctacac caaatagtgt taattcttct agcaacacta taaatttagg tggaatatct
    29941 ataaacgttc aagagagtaa taacaaagaa gaaatgatac aagaaatatt atctcaagta
    30001 gaagatggac taagagaagc attacaagac attggataat gtcgaattgt tgttaaaaaa
    30061 atctccctca taaatgatat aatattgtta aaatatgcaa atttatgagg gggaaatttt
    30121 atgtggcaga aatttaaaag tctaaatact ttcttaaaag ttattatagg tattatagca
    30181 atatgtttct taccaatcac tttagctgtt ttttcaattg aatttttaat gaaagctatt
    30241 gaagctaaaa ataaaggaaa aatagtctta ggatgttttt tagcattatt aacattaagt
    30301 tgtgctagga ctgtttatct tacaaaagat ttagatgata aaaattctac tcaagtcaat
    30361 caagtttcta gtaataaaga taaagaagat gctgaaaaga aagcggaaga agaaactaaa
    30421 aaacaaaaag agcagaaggc tcaaacagag aaacaaaaaa aagatgaaga agctataaaa
    30481 ttagtagaac aacaaaaaaa gaatgaagat gagcaaaaat cattggaaaa taagaaaaac
    30541 aatgaaccaa caaagttttt taatgatgta tacttaaaaa ataaaaattg tgttaacatg
    30601 gcatcttatg aaggaataaa atcaaacata gacatatttg gctataaata taaaattata
    30661 gaaccaaacg aaactacatt aagacagatt gatgtatatg acaatcaaag caatgatact
    30721 gttcaatttc aattttatga aaatgatact ggagaagaag tcgtaacagt tatgcaatac
    30781 aataaaaaag atgaacctga caagtccgtt gctataagtg atgattttca tatatatcca
    30841 ccaaaattta ataatcatat taataataca aatacagaag ttaatagcat agaagaacaa
    30901 ataaatttta tgtttaattg atatatgtat aatactttaa agttcttaca acttgtagga
    30961 gctttttgtt gtaaaaaaga ggtgataact tggtaataga catataccta aaaaatgaaa
    31021 aagaaaaaat agatttccat tttccggtta atccacaaga ttctttatct attaaaaaag
    31081 aaaaaaggtt tgaaactgta gatatagtaa atttaggtga atttgacatt aaaaaagaag
    31141 gggagaagat aagagaaata tcattcaaga catttttacc caatttatat gatgcttctt
    31201 attgtagata cagtgagtta aaaaatccaa tcgaagtagt tgcaatgctt gaaaaatggg
    31261 tagaccaagc cgaaccatta cgcttaatag taactggttt tggttacaat gggttagtta
    31321 caatatctag ttttagcaat actcaaacag caggaagaga agaagataga gatattgaga
    31381 taacatttag aacttacaga gaactgaaga tagagacatt aaaaaaagaa actaaaagta
    31441 atactaaaac agatttaaaa gacaatagac ctaatactca aactaaatct aaaatatata
    31501 cagttacatc tacagacacg ttatggagta ttgcaaagaa atttttaggc aaaggttcaa
    31561 ggtggaaaga gatttacaat ataccagaaa ataaaaaagt tattggtaaa aatccaaatg
    31621 taataaaaaa agggcaaaag ttggtgatac cttctaaatg aaaataatat taaatggaaa
    31681 atatgacatt gcaaatttta atgaaggaat aacattaagt gaagctatag acggagttgc
    31741 atataagatg gatgtatcat taatagaaac taaagctttg caagatataa atattaaaaa
    31801 aggtgataaa atagttctaa ttgatattgc gtatgagagt aaaaaagaag aaacaatctt
    31861 tgatggagtc atatgggaaa ctagaagaag tgaaaagagt aagaaactaa cattgtcttg
    31921 tagagaaaga acagtctata tggaagaatc agaagaacaa tatagtttta aagaaaatac
    31981 agcaacacag agaattgaat attactgtaa acaatggaat ataccttact acaatttagc
    32041 caacacaggg aagaaacttg ctaaagtaat acataagaca aatatcttag atatgattaa
    32101 aaaagactta aaagaaacag caacaaaagg tggagattta tttagggtaa gaatggataa
    32161 taaattaaaa ttgtttaagc ttggaactaa tgcaaatgta tataaattag atagtatatt
    32221 agaagatgca aattttacaa gtagctttaa tgatgcagta acaagtgtaa aagttttagg
    32281 taagagtaaa gacgaaaata caaaagcacc tgtgattgga acttataaaa aagattctga
    32341 taaatatggt actctacaaa agattaaaca agatgaaaag ataaaaaatg ctaaagaagc
    32401 taagaaagca gcagaagcaa tgttcaatag tggagaggaa acaataagtg tagattgtgc
    32461 agtagatata aataggatac gtgcaggtga taaggtatct ctaaaaggta aggagtatta
    32521 tgttatagat gttactcata cactagattc tactccaaaa atgaaattaa atgttggtac
    32581 actagattat ataaggagga aattttatac taatgactga tgctagattt aatggagttg
    32641 ctagaatatt gaaagaaaaa atgaataaaa gtgtagcaaa tggcactttt ggaatgggtt
    32701 gtgaacttgc agaaataaca gcaaaagggc ttaaagttaa tggttataaa gatgaaatac
    32761 aagattattt agtgttagag agtttaatat taaaagagga ttattttacc ctttcagatg
    32821 aaatattagg tgcagaatat agacataaac ataaaataga aactccaaaa gaattaaaac
    32881 cactaagtat aggtgataag gtgttagtag ctattatggg agctgaattt ttagtaattg
    32941 ggagggttgt aaatgccaaa cctatttccg cagaatgaaa cttttgaaac tgtagaatta
    33001 aaaaataata ataaaaatga actggaccta aaggggtctt ttttatttga ctttataaaa
    33061 ggtgaatttg tcaaaaatgc agatggaaca ttaaaaagat gtaacaaggt agaggcatat
    33121 aaacaatggt gtcaaaaagc tatattaaca cctagataca aacggtcagc ttattctagt
    33181 gtatatggaa gtgaaataaa agacttaatt gctagtaatc tatctcaaag tgctaaagaa
    33241 cttgaaatat ctaggttaat aaaagaaact attttagttc atccatatac aaaagaagtg
    33301 agtaatttta tattcaattg gcttgaaaat agtagccttg ttaattatga atttgatgta
    33361 ctaacaatag atgatgaaaa tatagtaatt gatggcaata taaaaaggta ggtgattata
    33421 tggaaagaga actacctata ccagtatttt taacggaaga tgaagaagca atacacgaaa
    33481 gaatgttaag taactttcaa gatgtttcta tcttagaagg agattttgtt tatgatgcaa
    33541 caagacctac agcagaacag atagctgaat taaaacaact aggactacaa aataatttaa
    33601 agattgcatt tcctcaaact agctatggag aatacctaga atggttggga gaatgtaaag
    33661 gagtatttaa aaatcaacca actaaatcgg ttggagttat tacttttata ggcatacaag
    33721 gaactataat tacaaaaggg actattgtta caactatagc tacagatgaa aaacaaagta
    33781 tagagtttga gcttcttgaa actaaaacta taggagaaaa tgaaacagta gatattaaag
    33841 cagaatgtag agaaactgga actataggaa atgtatctca aaatactgta actgttctat
    33901 taggctctat taatgggatt aaatctgtta ataataaaga agatttcaaa ggtggaacag
    33961 atatagaaga cgaagaacat tttagggaaa gagttcttgt agcagaacaa gaagacaaac
    34021 ttagtggagc taattcagat tatattcgtt gggctaaaga agttgatgga gtaggatatg
    34081 cttatgtagt tcccgaatgg aatggagcag gcacagtaaa agtattaata ctagacaaaa
    34141 atagaaaagc agcaacacaa gaattgatag ataaagttca agaatatata tatccattaa
    34201 atgtatcaga agaagaaaat agagacggga aagctcctat tggggcttta gtaacagttg
    34261 taacacccga aacactactt attaatgtaa aagctagttt tatatttagt aacagtttta
    34321 gtgaagaaac agtattgaac aatctaaaag ctaagataga taaatattta gataagattg
    34381 atttaggtgg aacagtctca tacaatgcta tacaggcaat agtaggttct atgatgttga
    34441 cagacgaagg tatacaggac ttttctaatc ttactataaa tgatgtaaaa gaaaatataa
    34501 aattgcagga ccaagtggtc ggagtagggg aaataactaa cgaggtggtt ggatgatagc
    34561 ttctaaaaaa ggtagagaaa tgcttcttac attgtcaccg atatatgagc aaagtttaat
    34621 tatgaactct atatatgaag ctataggaag cgaatttgat aatttagagt tattaaataa
    34681 agaaatagag ttacaattat ttcctcaaag tgcaacatgg ggacttgaat tttgggaaaa
    34741 tagagtaggt ttatctacta atctagatga agctatagaa actagaagaa gaaaagttat
    34801 tgctaagctc caaagcaaat atattatgac acctaaaaga atgtctatga tacttcaatc
    34861 ttatacaggt gcaaatataa aaataaatga aaacatattt ccatatactt ttagtgttga
    34921 attaactagt actcaaggtt tccctagaga tttagaagat ttatataaga gagtaaatgt
    34981 tataaaacct tctcatctat ctgtaagtta taagttagta tctttattaa aaagtaaaac
    35041 ttattttgca caaacaacaa ttatgagtga agaaataaca gtatatcctt attcaagtaa
    35101 agaagtaaaa gctaatgtta aagctaagtt tgcattagct cataacatga gttcagaaat
    35161 attgacagta tatccaaaag gaggtggtat aaatggctga tgaacaattt tacactatac
    35221 ttacaaatat aggtaaagct aagattgcta atgcaggaat gctaggtaaa tcagtagtac
    35281 tagagaagat tcaagcaggt gatggaggag gaagctatta caatccaaca gaagaccaaa
    35341 ctgcattaaa aaataaagtt tgggaaggta atataaatgc gtttgacaag gatgaaaata
    35401 atcctaattg gattattgca acagcatgtg tacctggttc aataggtgga tttacagtta
    35461 gagaaatggc tctcatagat aatgaaggag atatgattgc agtttgtaaa agtcctgaaa
    35521 catataagcc aaaagttggc aatggagcta tgaaagattt gtatttaaaa tttattatag
    35581 aggtatctaa tgtagagaaa gtgaccttag ttgttgaccc aactgcgata tttttaacta
    35641 aaaaagatga agaaaaaata ttaacaaata tcaataagtt agacactaaa attgatacaa
    35701 cgaaaacaga gttaacaagc aacatagaaa ctactaagac agagttaaat ggaaaaatag
    35761 gtgatacaac actacttgaa acaacagata aaacaaattt agttagtgca attaatgagg
    35821 tgaaaactag tgtagatagt atagaaacaa cagcagagaa aacaactata aaagatacag
    35881 ataatttgtt tgaaagtgac aatgtagagg aagcattaaa tcaactagca agaaattata
    35941 atacattatc agaaaaaaag aataatttag aaacagaagt gaatggacag agaataaaag
    36001 gaatttcaat agcaaataat ttaatagata tgatataagt gaggtggaga tatgacagaa
    36061 aagttgactg aaaattctag tttaagtgaa cttatgatag catttgaaag tataaaaaat
    36121 gacttacaga taggaaaaaa taatgtggca agtacattag gaagcccttt tactgggaca
    36181 gataaattgg gaacaactaa aacaaaaata gaaacattaa aaaatgtatt agtggaaaca
    36241 attaattcta aaaatgtttc agcaacatca tctgaaacat ttactaattt gattgaaaaa
    36301 gttaactgga tttttcaatc aatagaaatc ttttctttaa aaaatagaat ccaagctaca
    36361 attgtaaaca ctcctagcat cgtttacaat gaagtatcta gtataaaagg cacattaaga
    36421 ttcacaggca aacttcaagc gtctaaaatg cgtgctgatt atgcaacagc aaaaatagag
    36481 ctagtatgtg gaaaccgaaa agaatacttt tatgttactg acaatactcc tagtgcatct
    36541 tccactattc taacatttac aaaagatatt attgttgaga agggcatgga tataaaagta
    36601 caaatattat taacatctgt aggagcagga aatttagatg gttcatatgc agctcgtgca
    36661 gaaatagaag aattaaaaat attaaggtag gaataaatta tgaaaaaaca agtatattat
    36721 aattctttag atgaaaaaga aaaaataatt agtgaaaact ctaatttata tgttatagaa
    36781 atatatgaaa ctttaaatga aaattattta gtgttatcaa gtagtccaat agaagatgaa
    36841 aaacttagtt atgaagaatt agaaaatgaa ttattaatta tgactaatga attacaagga
    36901 ggattgttat aatgaatata aatgaagttg ttgtaagaat attagcagag agaatcctaa
    36961 acaaaggatt gaaccctttg aaaaatcgag aatttgaatt agatgacgta actaatgtag
    37021 agtacagaaa agctgtagag gattatataa ttagagaaag tggagtagta gaaggagcag
    37081 aacctacagc ataggtaatg ttcctttttt aattcaatta attaggaggt tttcatgaat
    37141 gaagaacttt tcaaagagaa tttgaaacga cacgaggtaa caataaataa acataatgat
    37201 gaaatagacg aattaaaagt agcaaatata gagtctaaag cagagttaaa agcattatgt
    37261 gagaatctaa a
//
LOCUS       DAEMHW010000016        36270 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEMHW010000016 DAEMHW010000000
VERSION     DAEMHW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.016"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(145..954)
                     /locus_tag="KPK67_000705"
     CDS             complement(145..954)
                     /locus_tag="KPK67_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2457708.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(1038..1571)
                     /locus_tag="KPK67_000706"
     CDS             complement(1038..1571)
                     /locus_tag="KPK67_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG2457709.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(1721..3070)
                     /locus_tag="KPK67_000707"
     CDS             complement(1721..3070)
                     /locus_tag="KPK67_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG2457710.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINVYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGNSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGADLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(3082..4464)
                     /locus_tag="KPK67_000708"
     CDS             complement(3082..4464)
                     /locus_tag="KPK67_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG2457711.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            4708..5922
                     /locus_tag="KPK67_000709"
     CDS             4708..5922
                     /locus_tag="KPK67_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG2457712.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVNFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            6099..8012
                     /locus_tag="KPK67_000710"
     CDS             6099..8012
                     /locus_tag="KPK67_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2457713.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            8283..9257
                     /locus_tag="KPK67_000711"
     CDS             8283..9257
                     /locus_tag="KPK67_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG2457714.1"
                     /translation="MRILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            9278..9553
                     /locus_tag="KPK67_000712"
     CDS             9278..9553
                     /locus_tag="KPK67_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG2457715.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            9566..10729
                     /locus_tag="KPK67_000713"
     CDS             9566..10729
                     /locus_tag="KPK67_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG2457716.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKTGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            11066..13345
                     /locus_tag="KPK67_000714"
     CDS             11066..13345
                     /locus_tag="KPK67_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2457717.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQGISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSVVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKTLEETNMDSFS"
     gene            complement(13446..14780)
                     /locus_tag="KPK67_000715"
     CDS             complement(13446..14780)
                     /locus_tag="KPK67_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2457718.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGININLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTS"
     gene            complement(14792..15574)
                     /locus_tag="KPK67_000716"
     CDS             complement(14792..15574)
                     /locus_tag="KPK67_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2457719.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(15589..16611)
                     /locus_tag="KPK67_000717"
     CDS             complement(15589..16611)
                     /locus_tag="KPK67_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG2457720.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGAKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(16617..17630)
                     /locus_tag="KPK67_000718"
     CDS             complement(16617..17630)
                     /locus_tag="KPK67_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG2457721.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(17758..18696)
                     /locus_tag="KPK67_000719"
     CDS             complement(17758..18696)
                     /locus_tag="KPK67_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2457722.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            18966..19430
                     /locus_tag="KPK67_000720"
     CDS             18966..19430
                     /locus_tag="KPK67_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457723.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTGKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(19542..19859)
                     /locus_tag="KPK67_000721"
     CDS             complement(19542..19859)
                     /locus_tag="KPK67_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2457724.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(19872..20603)
                     /gene="chbG"
                     /locus_tag="KPK67_000722"
     CDS             complement(19872..20603)
                     /gene="chbG"
                     /locus_tag="KPK67_000722"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBG2457725.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(20629..21939)
                     /locus_tag="KPK67_000723"
     CDS             complement(20629..21939)
                     /locus_tag="KPK67_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG2457726.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(22016..23329)
                     /locus_tag="KPK67_000724"
     CDS             complement(22016..23329)
                     /locus_tag="KPK67_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2457727.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEVYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(23351..23662)
                     /locus_tag="KPK67_000725"
     CDS             complement(23351..23662)
                     /locus_tag="KPK67_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2457728.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(23862..24566)
                     /locus_tag="KPK67_000726"
     CDS             complement(23862..24566)
                     /locus_tag="KPK67_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2457729.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(24814..26472)
                     /gene="treC"
                     /locus_tag="KPK67_000727"
     CDS             complement(24814..26472)
                     /gene="treC"
                     /locus_tag="KPK67_000727"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBG2457730.1"
                     /translation="MLDFKKSVVYQIYLKSFNDTTNNGLGDLNGITEKLDYLEFLGVD
                     YVWITPFFVSPQNDNGYDVADYYNIDPIYGTMDDLENLIKEAKKRNMGIMLDIVFNHT
                     STEHEWFKKALKGDKYYKDFYIFKNPKEGKSAPTNWESKFGGNAWEFVEKFNQYYLHL
                     FDKTQADLNWENPNVRKELKNILKFWIDKGIKGFRFDVVNLISKPEKYEDDLQGDGRH
                     FYTDGRKVHQYLKEMCEESGVLENDMLTVGEMSSTTIENCIKYSNPNEKELSMCFNFH
                     HLKVDYKNQNKWMLQDCDFIKLKKLLNEWQIGMEKGNGWNALFWCNHDQPRIISRFGD
                     DKKYYKESAKMLATMIYLLRGTPYVYQGEEIGMTNAYFTNIKQYVDVESLNYFDILKS
                     QGMKDGEIYNILQERSRDNSRTPMQWNNSKNAGFSESKPWIEVINNYRNINVENSLKD
                     KDSIFYYYKKLIELRKKYDVVAYGDFKPLLEENDSIFAYKRCYEKESLVVLNNFYDKE
                     IDVILDNEELEKYKCIISNYGDRKVTSTITLRPYECMALFKNEK"
     gene            complement(26535..27968)
                     /gene="treB"
                     /locus_tag="KPK67_000728"
     CDS             complement(26535..27968)
                     /gene="treB"
                     /locus_tag="KPK67_000728"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBG2457731.1"
                     /translation="MGKNKILNSKESISQIIEYVGGKENIQSATHCVTRLRLILKDTN
                     KANINKLEGIDIVKGCFNAGGQFQIVIGVGVDKVYKDLIEQTGAEEVSKEEIKQQVNE
                     KSNKLQKFIRVLADIFIPILPAIVASGLLMGVNNMIANPGIFYEQSVIEVNTGIAGIS
                     GIINLIANTSFTFLPALIGWSAAKKFGGNPLLGVVLGLVLVHPALMSAYDFAANPSAA
                     PTWDIFGMKVAQVGYQGQVIPVLIATYILTKLELFLKKTVPDAVQLVLVSPIALLITG
                     MLTFVVIGPITMTGANMITNGVVYLFEVAPVFAGALYSFISPPLVITGMHHLFLGVNL
                     QMAGTLGYVTLWPISETVTLAQGAATLAMFFILKNNKNLRGVSITSTISAWLGITEPA
                     IYGINLRFRYPFIAVMVASAIGGAFISYHNVKATAVGVGGVFSFLSVMPQYWGVYFTG
                     MAITFVITIGLTYLFYKSGKFKTNDNI"
     gene            28215..29954
                     /locus_tag="KPK67_000729"
     CDS             28215..29954
                     /locus_tag="KPK67_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457732.1"
                     /translation="MNIKEIPFSYFGSYMSISYSDELGFTLKSLRGKSKQNMDVLRII
                     PTVNNIPVDYEFEADYFSILIKFNNGSISICFDGDSKILFYGKGKNVGLKLDTLPVYN
                     FEYNYLMGKKDSEYCVINSYKNLTKFLVFSVDGKVNLSQNIFIDDCASTNKANNTSII
                     DVNNNTDNEFTCIIEDIPTHMKVPDYKEYNFETSLNNTKKLFNEYYLAQPKVDEKYKN
                     SLLRASYINWSSFVKPQGYLKRYTILASNSTFLGAWSWDHCFNALALAGVNDKLAFDQ
                     MAVLFDYQDEYGQIPGPLSDSTIRWNFTKPPIQGLFFNKMMKKINFTKETLEEIYNYI
                     EKQVLFYIKFKDSNMDGIFEYHHGNDSGQDNSTIFKGVMVVDTPDLTAFIINAMDMLA
                     KVAEKLNKYSEKEYWANKADELTKLFLSYFIVDDLPVARETLTGKILPNNALLPLVSL
                     VLGNRLPKYTRDKMIDLIISERYLTKWGIATEAIDSDEYQDDAYWRGPIWAPTTLLFV
                     EALEECGENELAIDIAKRYCELVDKNGFAENFNAITGEGLRDKSFTWTASTFIYLAAK
                     VYEYNTQRINNIK"
     gene            complement(29970..30701)
                     /locus_tag="KPK67_000730"
     CDS             complement(29970..30701)
                     /locus_tag="KPK67_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBG2457733.1"
                     /translation="MGRAKYYDIYIDLKNRIENKEYLYQDLLPSEYKLIEEYSCSRNT
                     IRRAIQELANQGYVQSKHGKGVFIIYRANTQSEFLLGGTESFKESAIRNNKEYKTKVV
                     HFEEIIIDDKKSQITNFEVGTEVYYIQRVRILENKPSIIDHNYFMKDIVKDINEEIAS
                     NSIYEYMENELNETIVTTKRTMTVEKATKLDKKYLELGDYNCVAVVSNNTYNAQGIMF
                     EYTQSRHIPDGFIFFQQSQRINNIK"
     gene            complement(31143..32861)
                     /locus_tag="KPK67_000731"
     CDS             complement(31143..32861)
                     /locus_tag="KPK67_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG2457734.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(32866..33747)
                     /locus_tag="KPK67_000732"
                     /pseudo
     CDS             complement(32866..33747)
                     /locus_tag="KPK67_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     regulatory      33970..34065
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(34279..34455)
                     /locus_tag="KPK67_000733"
     CDS             complement(34279..34455)
                     /locus_tag="KPK67_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457735.1"
                     /translation="MKTLMFILVIAYYITIIIKSITTIVKSITRLLDSISKLKESLHK
                     LRNRPDCHQDDFNE"
     gene            34712..34900
                     /locus_tag="KPK67_000734"
     CDS             34712..34900
                     /locus_tag="KPK67_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457736.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHGYSKK"
     gene            35776..35958
                     /locus_tag="KPK67_000735"
     CDS             35776..35958
                     /locus_tag="KPK67_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457737.1"
                     /translation="MSKDMIDECVHIMTAYITSLKEYYSFIETQIDDFIKKYGEDIVE
                     SCLHRIMILLCECGLV"
     gene            complement(36040..>36270)
                     /locus_tag="KPK67_000736"
     CDS             complement(36040..>36270)
                     /locus_tag="KPK67_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2457738.1"
                     /translation="ADILGLYYKREKESYLVTDIKDYKAHRTQNLYVIGGVTCNKMKE
                     MSKTTGEKFTQLYGNDVWSTMDKAIEFVKEKL"
BASE COUNT        12654 a         5796 c         4551 g        13269 t
ORIGIN      
        1 attttaggaa tatataataa tagggtttca gaacttagtt acaagttcta aaaccctatt
       61 tgtttagtgt aaaaaacatc ataaaattta gcttaaatct tttcacataa tattactctt
      121 tttctaagtg ataataaaat atatctattc ctctaaaaac tgtaaaaatt cctgattaaa
      181 tagtttaagc tcatcataga atatagcatg accactatac tcaaaagtat ataaaattga
      241 atcttctatt ttttcattca taaaaactgc aaattcatac ggacatattt catctaattt
      301 cccatgaaat ataccagtag gcactcttac acattttaaa tcattaaata attcttcatc
      361 tcttagagat acagcagttc caatagtgcc aatacctgat gcactaaaac caatatcatt
      421 aaaccatctt ctaaagcttt ctggtggatt tgatgcaaat actttttcgc caaaatcggt
      481 taccatttct ggtctatcag tacatgcttg tgctatcaat ttatttacat cttcacgtgt
      541 cataccatat ggaaattctg gaggtctttg tacaaatgat ggtgcagcag ctgcagctaa
      601 taccaatttt gacactccat atccattaaa taaactcata tacctaagta cgattgctcc
      661 tcccattgaa aaaccaacaa gggtaaagtc ttttaatcca atagcatgta ccaccttata
      721 aatatcgtct gctaattggc tataagtata tcctcctgat gttgcatcag atttaccaaa
      781 acctcttaaa tcaattgaaa ctgttctata tccgagtttt ggcaatatat ttgtttgata
      841 ttcaaatatc ttatgtccta agggccaacc atgtataaaa agtacagttt ttttagcact
      901 cggattcaaa tcatatactg ctatgtttat atcattcaca tatacataat acatatcaac
      961 agcaactccc ttcaagaaaa tgtatgatgg ttattttata tgtatgaata aaatgatgaa
     1021 taactaaatt ttcaaattta tttacaatac ttctctattg ccttttcaaa taaacttaca
     1081 tgaagttttt catctaggat tattctattt aatatagctt ttatataagg gtcttcaata
     1141 agacttatat ctttataata attattaata gccacatgct ccaaatgcaa atctagtttt
     1201 aatctgttac aaatagaatt tccataacat acaaaaccac cattccaata tgcaccacaa
     1261 gaacttgtag aacctctata tacaggattt ccacccaata atcttatagt ctttgctaat
     1321 atctccatat gtaacatttc tgttatagat atatttatcc acatttcact taactctcta
     1381 tgcgtttcat ctaaatcaaa attatggtat agatattgat ttacactcgt aaattcactt
     1441 gatactccgg catagtcatc catcaataat tctgcatagt acttatttgg acctaaaact
     1501 tttatctctg gatatggctc atcacttgaa tatccatttc gcttgtgcat gtcataatgt
     1561 gcatcactca taatatctcc tcctttaaat ttaatgtatg atattaattg tatgcatcac
     1621 aactttttca tataacttag ataataaaat ctacttaaat aataaaaagc catattaatt
     1681 aaaattaaca tgacctttta tccacaaaat tactttttaa ttattcatct tttccagcta
     1741 ctgatagctc tttaataaca actgaaggcg aacctatact actcattgga aattctaaat
     1801 ctgctcctat agcttcaata tcttttaaca ggtcaaaata gtttcctgct actgtaattt
     1861 gctctactgg aaatactttt tttccttctt tgatataaaa gcctttggca gctaaagaaa
     1921 agtctcctgt tactgaattt gcacctgaat gaagtcctgc aaagctagta accatcaacc
     1981 cttcatctat atcctttata atttcatcta aagatttatc tcctttctct atataaaaat
     2041 tagttggata tatgcttata ggtgatgaat atgaagattt tattccattt cctgtagtct
     2101 ttacacctgc cttattagcc gtttttaagt tatgaagtaa agttataagc tttccattta
     2161 aaacaacttc ttttttaaat gtagctactc cttcatcatc aaaaggagta gatgccatac
     2221 cattttctaa aagtgggtca tcaactattg taactatagg agatgctatc atgtctcctt
     2281 ctctattttt caataaagat aatccttttt gagctgaatc tgcattaaat atcccagaaa
     2341 atgtgctaag caaagatacc atagcttcat taagcaatat agttttatat ttacctgatg
     2401 gaatactatt accacctact ttagataaag cattatcaac accaagtttt gctatctcac
     2461 aaggttttat ttcttcaata gaatttgcta tattatatcc tattccatca tatttttgtc
     2521 cattgtcttc tatgatagga actacaaacc ctataagcat attagtttta ttgcttaaat
     2581 taagaccttt agtattagat ataccattac ttgatacaga ataagatata gtacatttag
     2641 ataaatttac caccttatct gaataagctt tagtctctct ttccatttct aaagctaaat
     2701 caatcaactt atctgcctct aaattttcta gctctttaga ataagtcttt acatcattat
     2761 agtgcttatc tccttcatat ataaattgta catcttcatt ttcaatagtg tttactccat
     2821 cttttacatt ttttatcaac atatcaacag ctttatcatc taaaatttct gtataagaat
     2881 aacctatctt tccatttacc ttacctctaa aagataaacc aaaagattta tcaagattgt
     2941 atttctcaac ttctccttca tatacattta tacttaaatt ttctcctgta taataatata
     3001 tttcacactc ttcaaaacct tcacttagag cctttttaaa taatatatct ttaaaatttt
     3061 taagttccac aattatccct cctatcttcc acctacagta atttctttta cccttatcat
     3121 aggttggcct acattagttg gtatacttcc tgatgatgag ccacacatcc cctgagcttg
     3181 ttttacgtta ttcccaacca tatctatatt cataagtata tcgctacctt ttccaattaa
     3241 actagcacct ctaactggct cttgtatttc tccatttttt attaagtaac cttctgaaac
     3301 tgcaaaatta aactctcctg ttacaggatt tacagaacca ccacccattt ttttagcata
     3361 aagtccatct ggtattgact ttataatatc ttctggtcta tcttctccag cagcaatata
     3421 agtgttagtc atccttgaag ttggtgcaaa tttgtagttc tgtctacgag aacttcctgt
     3481 aggattcatt cccattcgtc taccattaag tttatctatc atataagact tcaaaattcc
     3541 attttcaatc agtatattct tttgagttgg atttccttca tcatctatat tcatagaacc
     3601 ccagtagttt ggtattgtac catcatctat agctgtaatc tttgtggaag caatctgttg
     3661 acctaattta tctgcaaata cagagttccc ctttgcaact gatgtagctt ccaaagaatg
     3721 tccacaagct tcatgaaata ttactccacc aaatccatta tctattgcta ccatcatatt
     3781 acctgcagga caatttttag catgcaacat agtatgcgct actctagctg attcttttgc
     3841 ataatattct gggtctattt cttcaaatat ttcaaaccct ttacaacctc cagggccttc
     3901 aaatccagtt tgattttcat tgcccttaga tgcaattgaa cttatcccca gtctggttct
     3961 tattcttcta tcctcaacat atataccttc agtgtttgca attaatattt tttggtcttt
     4021 atccaaatag ctaacactta cttgagatat atcattacta aattcttttg cactcttata
     4081 tgcaaccttc ataacactta ctttatctct atttcctatt gaatttggat aaatttttat
     4141 attatgtatg ttatttattt taatactgtc atttaatatt acactttttc catcttccaa
     4201 tttacctaaa gcaacagcag ctttgtatgc tacatccaac agactattta atgaattgtc
     4261 acttgtataa gcatatatac ttttcaatcc tttaaatatt cttattccta tgccataatt
     4321 tcttccacct attgcatttt ctactttatt atctattaaa ctaatagaat tatttatagt
     4381 gtcttcttca aaaatctcag caaaatctcc tccagtaatt agacactttt ctaatacctt
     4441 agaagctata ttttttgaaa gcataaattt acctcctact ttaaattttt aattaacttt
     4501 tattcaatct ttttacttgt taacaaacat tatatactat attacttaaa ataaggtaat
     4561 tatgaatata ttatacatat gttttatact aaaatgttat cacattttat ctatacatac
     4621 ccatataact agataattaa aatgtataat attatttgct atagaaaata atacactcat
     4681 acataataaa ttttaggagt gaagtatatg aatcaagaat atgtaaaagg aaatcctatt
     4741 tttactaata ttaataaagt tccaagacaa tttccatacc ttactgatga tattgatact
     4801 gatgttataa ttgttggtgg tggcgtaact ggatgtattt gtgcttacta tctagctaaa
     4861 aacaatataa agtctgtcat tcttgaaaaa ggaaggattg cacatggaag cactagcgtg
     4921 acaacttctc tattacagta tgaattagat gacaatttga tagatttaac agaggttatg
     4981 actctaaaag atgctctaaa agcatataac ctttgtactt cagcacttga agaattagat
     5041 acatttatag agctatatgg aaataagtgt gattatgcca aaagagatac tctactttat
     5101 actgccaaca aacttgaagt taaggctatc aaagaagaat ataacttaag aaaagaaaat
     5161 ggatttgatg ttgaatatat tgatgaatcc acaaaccctt ttagctttga tttaaaatca
     5221 ggattgatag caaagaatgg aggacgagaa ttagacccat ataaatatag tcaccatttg
     5281 attgatgtaa gtttaaaaaa tggtcttcaa gtatatgaaa atacagaagt aaaaaaagta
     5341 gatttatcaa atgataaggt tactgctgaa gtatcgtatg gatacaaagt tcatggtaaa
     5401 aaattaattg tagctacagg gtacaataca agtttattta caaaaagaaa ttttgcaact
     5461 aagtcaaata cctttaatat agcaactaaa ccacttaaaa acatagcttc ttggaaaaac
     5521 aacattttaa ttagagataa ctgtgaccca tataattatc taagaactac taaagacaat
     5581 cgattaatta taggtggtga agatgtaaat tttgatgata tagaaaatga aactttagcc
     5641 aatgaaaagt acgatatttt agaacaaaga ttgaaaagta tgtttaagga tataaaagac
     5701 attgaagttg aatataaata ttgtggttgt tttgcttcaa ctttagataa tttaggtttt
     5761 attggtccag ataataaaaa taacaatcta tggtattgct taggttatgg agcaaatggt
     5821 atactctttg caatattagg aggtataatg ttaagtgaat tatatcttgg caaacaaaat
     5881 aaaaatatga aattatttaa agtagataga tttgataaat agatattata tattaaaaga
     5941 ctgtaaaaag atgaaacttt ataattctaa aagttaactt tgatacagtc ttttataatg
     6001 ttccttttaa aaatacttta tttataatta aatataaagt aaaatgttaa aatataactg
     6061 ataaaattta gtcataagat aatatttaag tttatttgat gaaatatgga ggaattatta
     6121 tgaaggaaat actcgttatt gctcccacta aaggaaccta tgaaaaatca attcacatag
     6181 ttaaaaaaaa caaatataca aatattgatg ttgtatttgg taatctaaaa gaaggtatac
     6241 ctcttgctga aaaaagtatt aatcatggta ctagaattat cataagccga ggtggaactt
     6301 acaatatgtt aaaagctact tacaatatac ctatagttga aattaaagta gatgcttatg
     6361 atattataaa aagctacaaa gaggtaaaaa attccaacga accatttgga ataattggat
     6421 ttaataatgt catctacggt ttcgatatta tagaagaaat attgaataag aaaataacta
     6481 tgattgaaat tgaaaaagaa gaagaaattt atgatgctat agaaaagtac agaaagaaag
     6541 gtataaacac atatatagga gataccactg ttgcacatat tgttaagaga cttaattgta
     6601 agggaatatt aatcgaatct agagaagaaa acatacttag agccattcaa caagcagaac
     6661 aaatactcga agcaaccaag gatgaacaaa aaagaagatt acagatagaa gctatgacag
     6721 attttgtaca tgatggaata ataacagttg ataaaaattt taaaataaca ttatttaata
     6781 agagtgctga aaaaatattt ggtataaaaa aagaaaatgc actttatcat aatgttgtag
     6841 atgttatccc aaacacagtt cttccacaag tagttgagac tatggaacct caaattggtg
     6901 aaatacaaaa tatagggagt acaaaaatta ttacaaatag agttccaata attgttgatg
     6961 gtcagattca aggggctgtt tcaacatttc aagatagcaa ggagataagt tcttttgaac
     7021 atacaataag aaggagctta ttaagcaaag gtctatctgc taaatacact tttgatgata
     7081 taattcatgc tagtgaaaag acaaaagatt gtatagaact tgcaaaaaaa tatgcttgtt
     7141 atgatactcc tatgattatt actggagaat ctggagttgg taaagaacta ttttgtcaaa
     7201 gtatacataa ctatagtaag cgtaaaaatg caccttttgt tgctgtaaat tgtgctgcca
     7261 tacctccatc tctaatagag agtgagtttt ttggttatga ggaaggttca tttacaggtg
     7321 ctagaaaaaa aggtaaagca ggtgtattcg aactagcaca tgaaggcaca atcttcctag
     7381 acgaaataag tgaaatccct cttgaacttc aaggtcgatt attgagggta ttgcaggaaa
     7441 aacaagttat gagaattggt ggtgataaag ttattcctat agatgtaaaa attatctgtg
     7501 cttcaaataa agacttaaaa aatatgatgc gcaatggttt atttagacaa gacctttatt
     7561 ttagaataaa tatactaaca ttatatcttc cttcattaaa agaaagaaaa gaagatatta
     7621 taaaattatc agaatttttt atacataaat attcaagcaa atacaataaa acgcctttaa
     7681 ttatcactcc aaatattaaa aaggcacttc ttgaacgaga atatgaaggt aatataaggg
     7741 aattggaagg tatgatggaa agggctgtta ttgtggaatc atttgactcg attttattag
     7801 aaaaggttag caatgaatat acaaataata tagttgataa agataatagt gattttgaat
     7861 gcagtaattt agatttaaaa actttagaaa aaaaatatat ctataaaatt tataacgaaa
     7921 ataatagaaa tactacaaaa acctgtgaaa ttctaaagat aactcgctcc actctatggc
     7981 gtaagttaaa agaaatagaa gatgatgttt aaaaataata catacctata tatatcaaaa
     8041 cgttttatgt ttaattttaa tacataagac gtttatttat tttcaataaa aaatacaatt
     8101 atatgataaa aagaatcttt gtaagtgaaa ttctcgtcaa tcatactttt aattataagt
     8161 aatctacagc atttcaatcg attattaaaa ataatcatga taactaaaga tacaaactct
     8221 atatattggc atagtaattg cttttataat acctatataa aaatgaaagg gagtgatact
     8281 agatgcgcat cttaaaatca gtgaaaaaga taccaggagg acttatggtc tgtcccttgt
     8341 tattgggagc actatttaat acagtatgtc cacaagctct tgaaattgga ggattcacaa
     8401 catccttatt taaaactggt gcaatgagta tacttgctct attttgtcta tgcaatggag
     8461 ctcaaatcaa tatcagacaa gctggtaaac cattgactaa aggtatagta cttacagcaa
     8521 caaaatttat aattggtgca gtattaggta ttattgttag taaatttatt ggacctaaag
     8581 gtataattgg tattacacct cttgccatag ttgctaccat gactaattca aatggaggtc
     8641 tatttgctgc tcttgcaggt gaatatggag actccacaga tgttggagca atatctatct
     8701 tatctctaaa tgatggtcct ttttttacaa tgcttgcttt tggagtaact ggacttgcaa
     8761 atgtacctat agtagcacta tttgcagcac ttataccaat actacttgga tttatacttg
     8821 gtaaccttga tgaagatata cgagaatttc ttgcacctgg tacaacatta ttaattcctt
     8881 tcttcgcttt tcctttagga gcagcattaa actttaatca aattataact gctggactac
     8941 ctggagttgt acttggttta ggagttacta taataacagg tatgggtggg tactttgtaa
     9001 tgaaacttat acgtgcagaa catccagcag taggtgcagc tacaggtaca acagccggaa
     9061 atgcagctgg tactcctgaa gctttagcag ctatagaccc tactctagct acaatagcag
     9121 ctgtttcaac agtacaggtt gctgctgcta ttattgttac agcaatatgt tgtccaatgt
     9181 tagtttccta tctagataaa agagaaaaaa agaaaaaatc tatttttaaa acaaataaaa
     9241 atattgaagt aaattaatac ttaaggaggt aaaattaatg aatgcaatta ttcatgcaaa
     9301 tgaaaaagat aatctagtta catgtgtcag acctttagtt aaaggtgaaa aagtttcaat
     9361 tgatggaaag tcttacatag taaatgataa tatacctgtc tttcataaga tggctacaga
     9421 agatgtaaaa aaaggtgatg ttgtatacaa atacggtgaa atcataggaa ttgcaacagt
     9481 tgacataaaa actggtgact atgttcatgt acataacatt gaaagtacac gtggtcgtgg
     9541 agataaaaaa taggaggaag ttaaaatgaa attaatgggt tatttaagag aaaatggtca
     9601 gtttggtatt cgtaatcatg ttttaattat tccaacatct gtatgttcaa gtgagacagc
     9661 tacaagaata gcatcacttg tgcctggagc aatagcaatc cctcatcaac atggatgttg
     9721 tcaaattggt tctgatatag agcttacagc aaagacatta ataggatttg gaaaaaatcc
     9781 taatgttgcc gcagttttag tagttggtct tggatgtgat ggtattcaag caaaagaatt
     9841 agcatctgaa atagctacaa caggaaaaaa ggtagattac gttgttatac aggaatgtgg
     9901 cggaacatta aaaacagttt ctaagggagc tgaaatagta agtaaaatgg ctcgtgaagt
     9961 gtcaaaagaa gtaagagttg aatttggtat gagtgaaatt acattagcac ttgaatgtgg
    10021 tggttctgac ccaacaagtg gaatagcttc aaacccttca ataggcgttg catcaaacct
    10081 tcttgtagat gaaggtggaa gtagcatact aagtgaaact acagaagtaa ttggtgcaga
    10141 acatttgttg gctacccgtt ttgaagatga agaaatgaaa gataagtttc ttaaatttgt
    10201 aagtgatgta gaaaaaagag caattgctat gggagaagat ttacgtagtg gtcaacctac
    10261 tccaggcaat aaagctggtg gactttcaac aattgaagaa aagtcacttg ggtgtatgta
    10321 taagacaggc aataagccat tcaaaggtgc tcttgaatat gctgatattg tacctcctga
    10381 taaaaaagga ttatatttta tggatactcc aggtcaagat atagattcta tcactggtat
    10441 ggtagcaggt ggtgcacaaa ttgtaatctt ttcaactggt agaggtactc caacaggaag
    10501 ccctatttca ccagttatta aaatcactgg gaatagtgat acatacaaca aaatgccaga
    10561 caatatcgat ataaatgcag gtagaattat aacagatggt gctaaaatag cagatatagg
    10621 tcaagaaata tttaatgaaa taatagaagt ctgtaatggt aaacatacca aagctgaaag
    10681 tcttggtcat agagaatttg gcatttacag aatttcatct acattttaga ttgtaacaaa
    10741 atatctttta taaatataat acttatgtag atacataagc taaaagttct ttctaatact
    10801 ataaaaattt tctatactga aaaggaactg tatcaaaatt attttgaaaa cagttccttt
    10861 tttaacctct tttactcaaa atattataaa ataagattta aacaataaac gtatcttgat
    10921 taatctctaa gaagacataa ataaagctaa atgtaaagtg ggataaataa aatttaatat
    10981 ctttgtatga aaagatattt ggtataatat gtctgagaga attcttattt gtaaacaaat
    11041 aaaggatgga ggatgaaaca aaagaatgaa ctttgaaaac acaaaaaata aaaaaataat
    11101 agctacttta ctattgacac tccttttaat cgtgtcatta acatgtgtca cttttattat
    11161 gaatctaaat aaagtaacac ataatgaaac agaacgatat cttgtagaag tttcacaagg
    11221 tatttccact acaattaatg tgaaatgtaa aagtatattt aggacactat attctatggg
    11281 agatacttat tttgagatta atgatagtga aaaagaagtc aatagatact tatcaaaaaa
    11341 attaaaatta tttgaatttg attggttagg tattatagat tctaatggaa attttgtaag
    11401 tgcccttaaa gatacaggta atgttaaagg acaagacttt tatagaaaag cccttcaagg
    11461 caaagaaaca attactcctg ttaaatcaca atctggtaca tatggaattt tatattctgt
    11521 accactatac aatggcgata aaattgttgg agctgttgct gcttggaata ctattgatac
    11581 tatgagaaat ttattaagtg tagaaagctt tcatggtgaa ggattttccc aaattatcga
    11641 ccaagatgga aattttattg taagttcagc aaacaaaaat gctccaaaaa atgtggtaaa
    11701 tttttttgac atgattaaac accaaggaaa acaggttgat aaaaataaac ttgaagaaat
    11761 gaaaataaac ttaaaaaaga acaaaactgg ttctatgtat tatacattaa atgatggtat
    11821 ttctaaggca atgcattatc ttccattaca agaagaaaac ttatacctcc tttcagttgt
    11881 tccaacaaaa acagcaagtg cacagtttga tttacttcta aaaaaagcaa tcttcatcaa
    11941 tattttaatt gttatactat ttttattgct tattatattg attatgttta taaatcaaaa
    12001 gaacagggca caacttataa aaatcgccta tgtagactct gtaactaaag gttacagtaa
    12061 agcacgtttt gatattgaag ctaaaaaaat tatacagtca tcttcatcag aaagctattc
    12121 attggtatct ataaatattc agaaattcaa acttattaat gattcttttg gtagtgaagc
    12181 aggagataaa acattaaagt acatacatga tgtcattagt aattatctag aaccaggaga
    12241 attgctttgt cgagtttcta atgaccagtt taatgtactt attaaaacta ttcctcaaaa
    12301 agaaattctt aagtacatag aaaatatcac caatgatatt aatagtttca ataaaaatga
    12361 agaatgtaaa tattttatag ctctatctat aggtgtatat aaaattgatg acccaaatct
    12421 acctttagtt tcaattcaag accgtgcaaa tgttgcacga aaaaataata aaggaaacat
    12481 tggaaatcat ccactttata catgtgtttt ttatagtgat ttagagcgtt taaaaatgat
    12541 taaagaaaaa gaaatggaaa atagaatgga ggaagcatta aataataacg aatttatagt
    12601 atatcttcag cctaaaataa gcctcaaaga taatactatt gtaggtgcag aagcattagt
    12661 aagatggcaa gacccaaata aaggtcttat accaccagac gagtttattc ctttctttga
    12721 aaaaaatggt tttattacaa aactcgacca ctatgttttt gaaaaagtat gcagtatgat
    12781 aagaaaatgg atagataatg gagaaactcc aatacctgtt tcagttaatt tttcacgtgc
    12841 taatttaaat aatatagatt ttttagaaaa atacagaatg cttcgtaata aatacaacat
    12901 tcctgctaag ctcttagaaa ttgaattaac tgaaacactt gtatttgaaa atctccaaaa
    12961 attaattaat ataatagacc aaatccatga agaaggtttt caatgttcac tagatgactt
    13021 tggaagtgga tactcttcac tcaatatgtt aaaagaaata aaagttgaca cattaaaact
    13081 tgaccgtgct ttttttagct cacctaatgc agataatatt gctgaaaatc atgtaataga
    13141 atctatagtt aatttggcaa aaaaattaaa tatgaatagt atatcagaag gtgttgaaac
    13201 aatcttgcaa atggattatc ttaaaaaaat taattgtgat atgatacaag gatatgtttt
    13261 ttcaaaacct ctgcctcaag aaatatttga gaaaatgact tttggaaaaa cactagaaga
    13321 aaccaatatg gatagtttca gttaaacaag ataaaaaact tgtctctata aaataatttt
    13381 aatcatttta taaagacaag tctttttatt ttctaatttc attttatttt ctagatttct
    13441 tatactcagc ttgttttaaa ctcccttttt aagaataata atacgattat catagcaata
    13501 aaatctgcaa ctggctgtgc caaccaaaca ccatctaaat taaattgaat tggaagcaca
    13561 atgattaatg gtataaataa aataaattgt cttaaaagag ttaaaaacat tgaatgttta
    13621 actttactta tacactgaaa atacacaggc cctacaatag aaatccccat tataggcaat
    13681 gtaattagat ttatgtttat acctcttact gctatatcca tcaaatctga atctttatta
    13741 aatataccta caaatacttc tgggaaaact tgtactacta aaaatccaaa tgacaatatg
    13801 acaactgaca ctatgataga taatattaaa gttttctttg cacgctcata ttgttttgca
    13861 ccataattgt atgatattat agtttgcata ccttgattca atccaaacac aggcatcata
    13921 aacatcaata aaatagatgt aagtgctgtc atagctccaa tagctaaatc tccaccataa
    13981 acctttagag aattgtttgt aaacatgtga gttatactta tagctacttc catagcaaaa
    14041 ggtgtcattc ctattgcaaa taccttcttt actatatctt tattcaattt taagttacat
    14101 ttttttagtt ttaaattgga cttccctctt ataaaataat gtattaccca aaagaatact
    14161 actatttgac agagtactgt tgctatagct gctcctttta tacctaaatt aaatacaaat
    14221 ataaatatag ggtccaatat caggtttaat acacatccta caatcattgt ccttgctgct
    14281 aattttggat taccttctgc cctaattgca ttattaagag aaaatgcaac taaattaaat
    14341 atacttccaa ccaatatgac actcatatag tcttttgcat aaaatattga atctttactt
    14401 gcacccaaaa taaaaagtat atcttctaag aaaactaatc ctaatatagt tataattaaa
    14461 gagataatta tagataatgt caaagcatta cctaatatct tctctgcttc ttctctattt
    14521 ctttccccta actttataga caatcttgtg ctagcaccta ctgataccaa cattccaaaa
    14581 gctattatta gagtaaatac tggcatagtt acaccaagac ctgcaatagc tatagagcca
    14641 ataccttcca tagaaccaat aaaagctcta tctactacat tataaagtga agttaccatc
    14701 attgctatta ttgcaggtat cgaataccta aataataaaa tccatatttt ttcatgtctt
    14761 aatgcttctt gattctccat gttgtcacca tttacaccat gtcataagtt atacaaattg
    14821 gtttatttgt tcttgggtct gttgctattt ctgcatcaat attaaataat tcttttagat
    14881 tatccttcgt cataacatca tatgcatcac cttcacaaac tattttacct ttcttaatac
    14941 ctatcatgtg gtcagcaaat cttgctgcat tattaagttc atgtattacc atgacaatag
    15001 ttcttccttg ttttttattt agttcatcta ataattttag tatctccaat tgatgtgcca
    15061 aatctaaata tgttgtaggc tcatccagta ataatatatc agtttcttgt gccaaagcca
    15121 tagctatcca tgccctttgt ctttgtcctc ctgataaatc ttccatattt ctatccttaa
    15181 attctgcaat tccagtaact tctaaactcc aatttacaat ctctctgtct tctttactag
    15241 aattaccaaa tccactttta tgaggaaatc ttccataaga aattaattct gaaacggtaa
    15301 gaccactagg agcttgaggg ctttgtggaa gtacagccat atcttttgct atttctttag
    15361 acttcttttt gtagatgtcc tctccattta tatatatttt cccactatta gggctaataa
    15421 ttctacctat agttttaagt attgtcgatt ttccacaacc attagcccct atgatggtag
    15481 ttatttttcc ttttggtata tttaaattta aattttttat aatatctgtg tttccatatg
    15541 atacatttag attcttagta tttatacagt tcataacaac accctttcct aatcatttac
    15601 caacattaaa taaataaagt acggcactcc tattatagaa actactatac ctactggtat
    15661 ttctataggt gctagaagat ttcttgatat agtatctgct attaaaagta ataatgttcc
    15721 tatcaatgct gatactggta ttaatctttt atgctttgct cctattattt ttctacctat
    15781 atgtggagct attaaaccta aaaatgaaat actacctgct actgctgtcg atacaccaga
    15841 taaaattact gcatatatta taagtcttct tctttgtttt tctacatcta caccaagact
    15901 tatagatact tcatcaccca aatttaaaac atctatatat ttagatttat aaatagttat
    15961 acccataaaa atcactataa atggaagcac agctattaca tatttccaac tggttcccca
    16021 aatgcttcct gatgtccatg ccataaccct attaaattcc tgtgttgtaa atttcaattg
    16081 gaatattgtc aaaattgaag taaaagctat gtttacacct ataccaataa gtagtaatct
    16141 tgacgaattt ataccacctt tccatgcaag agaatatata agaaatgctc caaataaagc
    16201 tcctataagt gcaacaatag gcattgtaaa tacagtcaag ttactcatac cttcatatac
    16261 attaccattc ataaggtata tataaactac tacaaacaat gcagcacctg aatttattcc
    16321 caaaattcca gaatcagcta aatcattctt tgtaactcct tgaagtatta cacctgctgt
    16381 tgataatgca gaaccaacca aaagtgctat tactattctt ggtaatctta atttaaatat
    16441 tgcaatttca tgattacgct gaccctgccc taaaaaagtc tttataacat ccattggttc
    16501 tatagaaaat gagcccatat ttaaactcaa taaaaaagtg ataaaaataa gcaccattaa
    16561 aattgatata acaagtaatg ttttagaatt tatttttata atatttttca tagtacctat
    16621 ttctccccct ttctaacaag gtatataaat attggcactc ctataagtgc tgttatagaa
    16681 cctactggtg tctcatatgg aggatttatc atcctactta atatatctgt atatacaagc
    16741 aatacagctc ctaatactaa agagctcggt attatatact tataatctga acctactatt
    16801 cctttagcta tttgaggaac tataagacct ataaaaataa tattaccaga cactgacaca
    16861 gatgctccag tcattaatat tacaagtact atacatataa atctaacaaa gttagttttt
    16921 tgtcctaatc ctattgccac ttcttctcca agacttaata tagttattct tggagccata
    16981 ataatagcta aaataaatcc aataccacca gctacaaata gcaattttat accttcccac
    17041 ttcactccta caagccctcc tgatatccag aaacttaatt gttgagatag gttaaaatac
    17101 atagatattg ctgatgccaa agaaattagc aatgtaccaa gagcgactcc tgcaagagct
    17161 aattttacat tattaactct tcctcttgat ttagaactta taaaatatat aaataatcca
    17221 cttatacttg ctccaataaa tgcaaacatc attacactaa aacttccatg tataagttga
    17281 ggaagttttt tttgaagtac cagtgatatc gaaattgcga atgtagctcc ttgtgtaatt
    17341 cctataacag atggttcagc tataggattt ctcgttattc cttgcattat tgccccagaa
    17401 actgctaaaa accctccaac aagtgcagct gctattgccc ttggaagtct aacatccttt
    17461 actattctca tatttattcc atcttccatt tttatcaaac tatctataac agttgataga
    17521 ttcatatctt ttgccccaat agttatggat aatacaattc caaatacaag tacaactata
    17581 cttactaata tcaacaaata agttatattt tttttactca gcttagtcaa ttatcctacc
    17641 tccttttccc ttttaatcgt cagtataata tcatctatta agtattttaa ttttaagatt
    17701 tattaaaaat aggtgtgaaa aatgaatatc attatctcac acctactaag ttaagaatta
    17761 ctttgttaat tcatttttaa ctgattctaa taataactct tttccaattg gattgtatga
    17821 ttgactgaaa aatggagcag catcaagtat tgttacatta ccatctttta ctgctcttat
    17881 ttgagcccag atagcactat tttctaaatc ttttttatct gcttctgtag caattacaac
    17941 tatatggtct gcatctatat ctgttaaacc ttccatactt accattggta gtgttattcc
    18001 atcctgtttt ggcatattct taggtctagc taatttcata tcatcattga ttagagtacc
    18061 tattccacca tcactaaata ccataaattg acctgaactt gccaacactg gtaaataagt
    18121 tttttctcca ttcttttcta taacttcctt acctaacttt gtagcttttt catcatactt
    18181 ctgtagccaa cttttagctt cttcttcttt gtcaaataac tttgaaacat cagttaactt
    18241 gcttctccag tcatttgcat aatctttcat cataactaca ggagctattt cttttaattg
    18301 gtcatatatt ttttcttgtc tttgactcat tattattaaa tcaggattta cttctaatat
    18361 agcttccata tccatagtat ccatcattga gtgcccaact atctttacac ctttaagctc
    18421 ttctcttatg taacttggta atttatcagt ttcatatgag tctacatttg ctgttgcaac
    18481 aggcttataa cctgctaata ataattcttc actacttcct gatatatcaa ctattttttt
    18541 aggatttgat ggtatcttaa cctctccttt aacagattgt acaactcttg tctctgattt
    18601 atctttatcc tctgtattct tatctgaaca tgctgtaaca agtactaacg tagttataat
    18661 agatataaat attgcaagtg attttatttt tttcatgatt acttctccta cttgtttatt
    18721 atacttacat gatattgatt atcataagca catatttatt atagcatttt tctattttta
    18781 gtcaatatta ttttgtaaaa ggataaaaat tcctaattaa tcttaattat atatagctta
    18841 gataaggttt tcttatatta aacagtattt ttttaaagaa ttggacacaa atacaatgac
    18901 ttttcagtta aacaatgata aaatgcaatt tatagataat gaatttagta gggaggattc
    18961 aaaacatggt acatttagta tattgtgata atgcaggaaa aaaaggagaa aaggtcttag
    19021 ataaaatctt ggctggaaca aaaacaatgg ttattagagg tgctgctggt agaaaaatac
    19081 cacatagtag agtatttgaa gatgagttac tttattttat ggaaaaagga actggaaaaa
    19141 tatctgcaaa agccactgtt aaaaaggtag aaaactatgt taaattaaca gaagatgaaa
    19201 tttctaaaat tttaaatgaa aatcaaccaa aattaaattt atctgaaaag caaaaagttc
    19261 gttggcataa aaaatgttta tgcttagttg aatttgaaaa tgttgaagaa ataacacctc
    19321 ttgattttga ccatcaagga aatatggatg attggcttat tgttgaaaaa attgaagatg
    19381 tagttgttgg tactagtata ccttacaatt atgagaagtc taaattttaa agtatcaata
    19441 acataatact atattgctaa actctactta aataaaaaaa gagtgcattt aataattata
    19501 aaaattattt ttatacactc ttttttaata ataattttaa attacttact ttctaatctt
    19561 tcatatacat ctataaattc ttctgctaat tgttgaaaat tcatagctgt cattaaatgg
    19621 tcttgtgcat gaataagtat aacacttatt tcagttttat taccattagc ttcattttgt
    19681 atcaattctg tttggaattt atgagcttta tgaattgatt ctttcgactt ttcaatacat
    19741 tctctagctt tatctatatt cccgctcttt gcttctttta ttgcttcaaa tgctagccct
    19801 ttcgcatcac ctgcaaatcc aattattcca aaagatatct ctatcatctt ctcatccatt
    19861 tattcgacct cctaaatatc tctatagctt gatatgacta atccattctt ttctaaaaat
    19921 tctttgactt ttttagatgt aagtatcttg tgctctttcg ttctatcaat agcataagat
    19981 gtagaattta aaatataatc atctaaaaaa gctgggtgag acataatatc cactacatca
    20041 tacttcatta tttcgtctat attttttata aaatattctt cacttacatt ttccttataa
    20101 aaactatcta ttaaaggaac tactttagaa tactctaaat tatattcaaa tccacctctt
    20161 atcggaagat tatacttggt tacaatcttc tctattactg gctttaaact tactaaagta
    20221 tggatatgat ggtgactatc taaatgagat atatctattc caagcccttt taccctatct
    20281 atttgagcac aaagttctct ataaatctca tcacaatcca ttttttcaat aatttcgtct
    20341 gtaatcctcc taataaaaaa accgtcttta tcaacaatag tttttaagtt agataataat
    20401 ggtctaccac aacttaaagt catatgaact ccacaattta aagttttatt ttctttaagt
    20461 aagcctactc catgttctac cccaggcata tttgccatca tagaagtaga cctaactata
    20521 ccattattat gagctgaaat tattccatag ttcacagctt cacaatatcc aaaatcatct
    20581 gcatttatta taattttagt cataccataa cctactttct atagacaact atctattaaa
    20641 tctaggaaga taatctttat gagcttccaa catttcgtct aatatagttt ttgctaagtc
    20701 atcagatgga atcaatgggt ttatacaaag tgccaaaaga gcagattcat aatcgccata
    20761 tacagctgct ttagctgcca atacttcaaa tgatttgatt tgacttacta atccatgcac
    20821 agaatctggt aaatatccta tacttattgg ttttggacca ttttttgtta ttacactact
    20881 tacttctact gcttgttcat ctttaaaatc tcttatagaa cccttattta atgtattaac
    20941 tacttggata tcttttttgt cattgtatat agaacttatt aaattacaag cagcatcact
    21001 atagtaagcc cctcctcttt tttcaagttg aggtggtttt acatctaatt tttcatcctt
    21061 atataattca aataattgtt cttccaattc tttaactact tgacctctag cttttccctc
    21121 tttaaactct ctcaactcat cctcaagcat ttctttagtt ttatagtagt atctatgata
    21181 tggacaaggt attgcaccca aagattttac aaattcagca ttaaaatcaa ttgcctttat
    21241 attttgcata cttatatctg ctttgacaaa tttatcaatt gcttcttttg taacatcttc
    21301 tccatctaaa gatacattta gaccataaac catatgatta agacctgcaa aatccatact
    21361 tattctgtca ctttctacat taaatagttt ggcaacatca ttttttaaat gaattggcac
    21421 gttgcataga cctatatatc ttctaaaatt agtatatttg aatacagcct ctgttattat
    21481 tcctgttgga tttgtaaaat tcaccatcca tgcatttgga cataattctt ccacatcttt
    21541 tattatatca aatataactg gtattgtgcg aagggcttta aataaacctc cagcaccatt
    21601 agtttcctgc cccataactc catgagataa aggaatagtt tcatctttta ttctcgcatc
    21661 taaaagacct actctaagtt gtgttgtcac aaagtctgca tctcttagag cttctcttct
    21721 atctaaagta agatttattt tcatatctac tccagctttt tttaccatcc tcttagctag
    21781 atttccaact atctctaatt tttcttttcc agcttctata tctacaagcc ataattcctt
    21841 tacaggtaac tcttcatatc tatttataaa accctctaca agctcaggag tataacttga
    21901 acctcctcca attgtaacta tttttagtcc ctttttcatc tcattatacc atccttacct
    21961 ataatttaat atactctaca aaaccctata catttattta ccgaaataaa actacctagt
    22021 tgttaatttt caatttatta gcttccattt tttcagatgc tatgacaaat ggaatatata
    22081 ttaatattga taatattaaa ttaaatagtg ctaaagctcc accagtccag tcacctgtag
    22141 ccattgctcc acctaatata ggaggtgtta cccaaggtat tttaggtaaa attacaggtg
    22201 ctactaatcc aaaatctatt gctagatatg atacagcact acatatcact ggtgcaagta
    22261 caaatggaat cataaatata ggatttaaaa ctattggaag tccgaatatc aatggttctc
    22321 ctatgttaaa tattgcaggt ggaccaccta atactatcat ctctttacgt cttcttccag
    22381 ctattaataa agctactatt aatcccaata ccattccaga accacctaaa tatacaaatg
    22441 catcaaagaa tgcacctgca agtacatggt atccttccgt tgctcctgct tgagccaagg
    22501 cagcattttc tccacctaat ttcataagta tagtttctgt aaaaggaagt gctatatttg
    22561 caccatgaag tccaacagtc caaagtatat gaattataaa tgcaataagc attgtagaac
    22621 ctagactatc agctacattt gatagtggtg ctcctaaata agtatttaaa atatctgtta
    22681 agaaacttcc attagaaagc atttttataa atattcctat tacagtaaat atcattatag
    22741 ttaacattcc tggtattaac tttgcaaatg atcttgaaac tgctggtgga actccatctg
    22801 gcatttttat agttaatttc tttaccttag ataatcttac aaatatctca gttgataata
    22861 atcctattaa tatagaagag aataaagctg ctgtacctaa gtaggtttgt tgtatcattc
    22921 cccatccttc aactactgta gttcctccct caggaactat tttaccaatt tgtggagcca
    22981 ttataaaaaa gatagataaa gctataagcc cagattgtaa cccatcactc tcataagatt
    23041 ttgctagaaa ataagcaaca cctattacta agaatactgc cattgtacct attgctcccc
    23101 accaaaggtc gcctccaaat gtggtccaat tttctccaaa tatacttgcc atcaaatttt
    23161 tgtatacttc caatggtaag ttatttatca atgttcccaa tgcacctacc atagttatag
    23221 gtatcattac tataaatgca tctcttactg ctactaagtg cctttgagct cctattttag
    23281 cagctacagg cactatatat ttatccataa atgacatgaa tttttccatt ttacatcctc
    23341 cagtacaact ttattttgaa ctatttaact ctacagctct atctaacaca gcttttccat
    23401 tacatcttcc ataatccatc atatttataa cttctactgg aatattatat ggttttgcca
    23461 tttcagttgc ttttcctaat acatatctaa cttgaggccc taaaagtaag acatcacaat
    23521 tttctatttc attttttaag ttcacttctg atatagccca tatttttgct tctataccgt
    23581 tttcctttgc tgcatcctcc atttttgtaa ctaataaact agttgacatt cctgctgaac
    23641 aagctaacat tatttttatc attttttccc ctccaaatta gattcattaa atatttttta
    23701 agtcataaat tttttaaact atacatttaa aatatgcttt tctattaatt ttttcttaat
    23761 gtatatatct tatttttaaa tgtatataca tttaattctt gatattatta tataccaaat
    23821 gtatatacat ttcaagaaaa ctttttcatt ttatttaaat tttattcgac aacagcattc
    23881 aaagttaatg tatatccata gtgatatgtg tgtgagtact caaaaatctc actattttgt
    23941 aacttacaaa tttggcttat atataaaagt ggctctggtt cttctaattt tagattatcc
    24001 atacattctt cattcgatat tacagcttgt acttctctct ctgacatggc tatcttgtat
    24061 ccacacactt cttcaacaaa attataaaga gaattttcac aatgagtctt atttaaattt
    24121 ggaaataact taactggcat ataagtaatc atatatgaaa ctcttgcatc attggctaac
    24181 cttactcttt ttatttccca aacattttct tctaaactta tattcaactt acttgataaa
    24241 ttttcatctg cctgaactat ttcaagagat accaatatat tagatacttt atagccttta
    24301 ctttccatct ctttagtaaa tccacttaca gacgatatat tatcatatat attatttgca
    24361 ataactatac tacctacacc tctttgtcta gttatatagc cttctttaac tagattatta
    24421 agtgcagacc ttacagtcat tctagttaca ttaaattctt cactcagctg tttttcagaa
    24481 gggattaaat ccccttcttt caaactgtct gaatttatta actctctaac atgattttct
    24541 ataactttat aaattggctc tttcatttaa ttcactcctt attaaatctt ttatataata
    24601 agataatttt atatacaaac aaagaaaatg tctatacatt taagataatt taaattttct
    24661 taatatagat tataatttac atatgtggaa aaatattaaa ataaggctat aatttttaca
    24721 atcatagcct tattaataaa tattaaatta taacttcaaa aataaaattt atttaaagag
    24781 tacacataaa tttttcctaa ttttaatttt aaactatttt tcatttttaa ataaagccat
    24841 gcactcataa ggtcttaagg taattgttga agtaactttt ctgtctccat aattagatat
    24901 tatacacttg tatttttcaa gctcttcatt atctaaaata acatcaattt ctttatcata
    24961 aaagttattt aacactacta aagattcttt ttcgtaacat ctcttatatg caaatataga
    25021 atcattctct tctaataatg gcttaaaatc cccatatgct actacatcat acttttttct
    25081 taattctatt aacttcttat aataataaaa aatagaatcc ttatctttta aactattttc
    25141 tacatttata tttctataat tatttataac ttctatccat ggttttgatt cgctaaaacc
    25201 tgcattctta ctattattcc attgcatagg tgttcttgag ttatcacgag atctttcctg
    25261 taatatattg tatatctcgc catctttcat tccttgagat tttaatatat caaaataatt
    25321 taaactctca acatctacat attgttttat attagtaaag tatgcattag tcatgcctat
    25381 ttcttcacct tgatatacat atggtgttcc tcttaataaa taaatcatag tagctaacat
    25441 tttagccgat tctttataat actttttatc atctccaaac ctagatatta tacgtggttg
    25501 gtcatggtta caccaaaata aagcgttcca tccattacct ttttccattc ctatttgcca
    25561 ctcattaagt agttttttta acttaataaa atcacaatct tgcaacatcc atttattttg
    25621 gtttttataa tcaactttta aatggtgaaa attaaaacac atagacaatt ctttctcatt
    25681 tggatttgaa tattttatac agttttctat agtcgtagaa gacatttctc ctacggttaa
    25741 catatcattt tccaatactc cactttcttc acacatttct tttaagtatt gatgtacttt
    25801 tctaccatct gtataaaaat gtctaccatc accttgtaaa tcatcttcat atttctctgg
    25861 tttagatatt aaatttacta catcaaatct aaatcctttt atacctttat ctatccaaaa
    25921 ctttagtata ttttttaatt cttttcttac attaggattt tcccagttta agtctgcttg
    25981 agttttatca aataaatgta aataatattg attaaatttt tctacaaact cccaagcatt
    26041 gcctccaaat tttgattccc aatttgttgg ggcactctta ccttctttag gatttttaaa
    26101 tatataaaaa tctttataat acttatctcc tttaagtgct tttttgaacc actcatgctc
    26161 tgtagacgta tgattaaata cgatatcaag catgataccc atatttcttt ttttagcttc
    26221 ttttattaaa ttttccaaat catccatagt tccatatata gggtctatat tataataatc
    26281 agctacatca tatccattat cattttgtgg tgatacaaaa aatggagtaa tccacacata
    26341 atcaacacct aaaaattcta agtaatctaa tttttctgtt attccattta aatctcctaa
    26401 accattattt gttgtatcat taaacgactt taaatatatt tgatacacta cactcttttt
    26461 aaaatctaac atattgtctc ctatctatcc aatacatatt aataccccct gatataaaat
    26521 caagggaatt atatttatat gttgtcattt gttttaaatt taccactttt ataaaataag
    26581 tatgttaatc cgattgttat tacaaatgtt attgccattc ctgtaaaata tactccccaa
    26641 tattgaggca ttactgaaag gaatgaaaat actcctccaa ctccaacagc tgtagcttta
    26701 acattgtgat atgatataaa tgctcctcct attgcacttg ctaccataac ggctataaat
    26761 ggatatctaa atcttaagtt tataccgtat atagctggtt ctgttattcc taaccatgct
    26821 gatatagtag atgtaataga tacacctctt aagtttttat tatttttaag tatgaaaaac
    26881 attgctaatg tagcagctcc ttgagctaaa gtaacagtct ctgatattgg ccacaatgtt
    26941 acatatccta aagtccctgc catttgtaag tttactccta agaataaatg atgcattcct
    27001 gttattacta atggtggtga aataaatgaa tataatgctc ctgcaaaaac tggtgctact
    27061 tcaaataaat acactactcc attagttatc atatttgcac ctgtcatagt tataggacct
    27121 ataactacaa aagttaacat acctgtaatt aataaagcta ttggtgaaac taatactaat
    27181 tgaactgcat ctgggacagt cttttttaag aataattcaa gtttagttaa aatgtatgta
    27241 gctattaaaa ctggtattac ttgaccttga tatccaactt gagcaacctt cataccaaat
    27301 atatcccatg ttggtgctgc ggatggattt gctgcaaaat catatgcaga catcaaagct
    27361 ggatgaacta atacaagacc taaaactaca cctaacaaag gatttcctcc aaacttcttg
    27421 gcagctgacc aacctattaa tgctggtaaa aatgtaaatg atgtgtttgc tattaaattt
    27481 ataatacctg aaattccagc tataccagta tttacctcta tgacagactg ctcataaaaa
    27541 attcctggat tagctatcat gttattaaca cccataagta accctgatgc aactattgct
    27601 ggtaatatag gaatgaatat atcagcaaga actcttataa acttttgtaa tttatttgac
    27661 ttttcattga cttgttgttt aatttcttct ttagatactt cttctgctcc tgtttgttct
    27721 attaaatctt tatatacttt atctactccg acacctatta ctatttgaaa ctgtccacct
    27781 gcattgaaac atccttttac tatatcaatg ccttctagtt tattaatgtt tgccttgtta
    27841 gtatctttta atattaaacg taatcttgtt acacagtgtg ttgcactttg tatattttct
    27901 tttcccccaa catactcaat aatttgagaa atgctttctt ttgaatttaa tatcttattc
    27961 ttccccatat tcatttatcc tttctttttt tcatcattac caacaagttg tttagacaag
    28021 tacttgtttg aacaacttca ttgtaatatt ttgcattaat ttagtcaata gttttttgaa
    28081 aacagtttcc tatttatttt ttgataaatt gactcttgat aattatttta ttatttggta
    28141 agttaaatat atacttactt gtataaacaa gttagattct atataaaata aattatgaaa
    28201 ggaatatact acaaatgaat ataaaagaaa ttccatttag ttactttggg tcttatatgt
    28261 ctatttctta ttcagatgaa cttggattta cattaaaatc actaagggga aaatcaaaac
    28321 aaaatatgga tgtactaagg attataccta ctgtaaataa catacctgta gattatgaat
    28381 ttgaggctga ttatttcagt atattaatta aattcaacaa tggtagcata tctatatgtt
    28441 ttgatggaga ttctaaaatc ttattctacg gtaagggaaa aaatgtgggg cttaaattag
    28501 atacacttcc tgtatacaat tttgaatata actatcttat ggggaaaaaa gattcagaat
    28561 attgtgtgat aaatagctac aaaaatttaa ctaagttttt agtattctct gttgatggta
    28621 aggttaattt atcacaaaat atatttatag atgactgtgc ttctaccaat aaagctaaca
    28681 acacttcaat aatagatgtt aacaataata ctgataatga atttacatgt attatagagg
    28741 atattcccac tcatatgaag gttccagatt ataaggaata taattttgaa acttcattaa
    28801 ataacacaaa aaaattattt aatgaatatt atctagctca acctaaggta gatgaaaagt
    28861 ataaaaattc acttttaaga gcgagttata taaattggtc ttctttcgtt aaacctcaag
    28921 gatacctaaa gagatatact atacttgctt caaactcaac attcttaggg gcatggagtt
    28981 gggatcactg ttttaacgca ttggctttag ctggtgtaaa tgataaatta gcttttgacc
    29041 aaatggcagt acttttcgac taccaagatg aatatggtca aattccaggg cctttaagtg
    29101 attctactat tagatggaat tttacaaaac cacctattca gggattattc tttaataaaa
    29161 tgatgaaaaa aattaacttt actaaagaaa cattagagga aatttataat tacatagaaa
    29221 agcaagtatt gttttatata aagtttaaag attcaaacat ggatggtatt tttgaatatc
    29281 atcatggaaa tgattctggt caagataata gtacaatatt taaaggagtc atggttgttg
    29341 atacgcctga cttaacagca tttataataa atgctatgga catgttagca aaagtagctg
    29401 aaaaactaaa taaatatagt gaaaaagagt attgggctaa taaagcagat gagcttacaa
    29461 aattattctt aagttatttt atagttgatg atttacctgt agctcgtgag actttaactg
    29521 gtaaaatact tcctaataat gctttactcc ctcttgtttc tttagtgcta ggaaatagac
    29581 ttcctaaata tactagagac aaaatgatag atttaattat tagtgaaaga tatttaacta
    29641 aatggggaat cgcaacggaa gctatagata gtgatgaata tcaagatgat gcttattgga
    29701 gaggtcctat ttgggctcct actacgctat tatttgttga ggctttagaa gaatgcggtg
    29761 aaaatgaact tgcaatagat attgccaaaa gatactgtga actagttgat aaaaatggat
    29821 ttgctgaaaa tttcaatgct attacaggag aaggtcttag agataaatct tttacctgga
    29881 cagctagtac attcatatac ttagctgcta aagtttatga gtataacacc caaagaatta
    29941 ataatataaa atagagtaca aattgtactc tattttatat tattaattct ttgggattgt
    30001 tggaaaaata tgaatccatc aggtatatgt ctagactgtg tatattcaaa cattattcct
    30061 tgagcattat aagtattatt gctaacaact gcaacacaat tataatctcc taattccaaa
    30121 tattttttat ctagtttagt tgctttttca actgtcatgg ttctttttgt tgttactata
    30181 gtttcattca attcattttc catatattca tatatagaat tagatgctat ttcttcatta
    30241 atatctttta ctatatcctt cataaaataa ttatgatcta taatagatgg tttattttct
    30301 aatattctta ctctttgtat ataatacact tctgttccaa cttcaaagtt agttatttga
    30361 ctttttttat catctataat aatttcctca aaatgaacta ctttcgtttt atattcttta
    30421 ttgtttctaa tagcggattc tttaaatgat tctgttcctc ctaataaaaa ttctgattgt
    30481 gtattagccc tataaattat aaatactcct tttccatgct tactttgaac atatccttgg
    30541 tttgctaatt cctgtatagc ccttctaatt gtatttctgg aacaactata ctcttctatt
    30601 aatttgtatt ctgatggtaa taaatcttga tataaatatt ccttattttc tattctattt
    30661 tttaaatcta tatatatatc ataatatttg gctcttccca ttttaacctc ccaattttat
    30721 atataaatat ttaattgtgt cttatttcta aaaatcatgt attaatataa tatattataa
    30781 gttaagcaaa aaccaaaata aatataaatt taaaactgga actaaacttt ttataaaaaa
    30841 cttcaactgt aaaaactatt aaaacattat ttttatgcga aacttaataa tttgtcaaac
    30901 ttagcttgat aatattctaa atccaaccaa tcttccagta acaccaaaaa ctttagattt
    30961 agagaaagtt tccatagaca tctctttatt tgataaatat aattatgcat attttcaaat
    31021 gtataataaa aaaggaaaat taacactatt aatattaatt aaaatttaaa aaagtctatt
    31081 ctttaaataa taaatcacaa gaatagactt ttttaaattt gtgtttttat aagataaatt
    31141 atttaattaa agtttgttct tttttctaaa cgattcttta taaattcata tattactttc
    31201 actaatatat ttgtgactaa tattaacaca gctagtgctg ctgcctgtgc agtactcccc
    31261 ttatcatcta gttgtacaat tgatattgca gctacttttg aacttgcagt atatataaaa
    31321 actaatgctg atattgtcac catggagttt aggaaaaagt acataaagtt ctctaaaact
    31381 gctggcaaac aaagtggtat agttacgttc ttaagaattg atgcgctgtt tattcccatt
    31441 gatttagcta ccatatccaa ttctttatct actttcttaa tagcagtagt agctgttata
    31501 aatgctactg aaaagaaatg aactatatta catataacca ttataagtaa tgttccatat
    31561 aatccattaa acaaattctt aatgtatatt gtatccataa aattaaactc taatttatta
    31621 aaaaataata cataagctat acctaaaact aatcctggta atgccatagg caatgtactt
    31681 aaaaaatacc caaatgattt taaatatgga gctttttctg ttttttcatt caaatatgca
    31741 aataagaaaa caaagctcgt ccctagaata gctgtcaata gtgacatttt tattgaattt
    31801 ataaatggtt taatcccatc aatactgttt ttcatagaaa aatgttccag tgtcagtctt
    31861 aaatcatatg gccaaactga tattaatgaa gataagatta cagttaacat tacagctatt
    31921 ataccaaatg caataattcc tacaacccct cctaaaatac tatctcttaa cttattagtt
    31981 ttaaccttat atggaattga ctttgaagtt atatatgaat tttgattttt ttcaattttt
    32041 ttagttacta taaacgatat tattgcaggt attaaaagta atattgatac agttgctccc
    32101 attggcatat tctgttgccc tataatttgc ttatatacat ctattgataa tacattaaac
    32161 tctcctccta tcacctttgg agctccaaaa tctgtaaaac ataatgtaaa cgatacgaat
    32221 gcagaactta ttaaagagta ttttatactt ggtattgtta tgcttataaa ttctcttatc
    32281 ttgcttatcc ccatagtatt actagcttca tatagtctat aatcggttga acttagagat
    32341 atataaagta tttgaaatga ttgaggaaat acatatataa tttgtgccaa tattattccc
    32401 actgctccat atattggaac atgcactcca agtaaattag ttaccaaacc ttgatttcca
    32461 aataaatata ctagacctat tgcatgcatc attgttggag caaacatagg aaacatagct
    32521 aatgatttaa aaaaaccttt aaatttaatc tttgttctag ttaatgcata tgcatatact
    32581 aatgcaagtg ttagtgatat tacagttgat ataactgata caaatactgt attttttaga
    32641 gaatttaata gagattcaga agccaagtat tcttgaaagt tttgtaatcc tacaaagact
    32701 ccatctttat ctaaaaaagc ctgtacaaat agatatatta taggtgctaa taaaaatatt
    32761 actaaagaca taactaatac tgtaagcata cattttttta tatctaaatt catttttgat
    32821 ttaagactta tacttttaga acttatagca ttattattca tacacttatg ctcctttaac
    32881 ttttggatac tttaaatagt atttttcgtc tatacttatt tttacaaggt caccttctac
    32941 taaatttaaa ttaacccact cttttataga aacatcactt attatgaaat tttcatttaa
    33001 tttatctttt atttcaactc tcaataaatt ccctctaaac tctatatttg atattattcc
    33061 ttggtagttt tcttttgtcg atttctctat ttgaacatat tctggtctta cagttaatat
    33121 agaatctcca ttatcaaaac agtttgtatc tcctataaat tgagctgtaa ataggttttg
    33181 aggattttgg tatatttctt ctggagtccc tatttgcatt atatctcctc catttagaat
    33241 agctatttta tctgccatag ttatagcttc ttcttggtca tgtgttacca ttattgtcgt
    33301 tatattaagt tctttttgta atcttttaat gtccattcta agcttgtgtc taacctttgc
    33361 atctaatgcg gacattggtt catctaaaag taaaaacttt ggttcaagag ctaatgctct
    33421 tgctattgca atcctctgtt gttgcccacc tgataatgct ttaggatatt tatgagcttc
    33481 attagtcaat ccaactgttt caagtacttc cttaacttta ttatctattt ttccttttga
    33541 aacttttctc tcttttagtg gaaaagctat attgttataa gcagtcatat tcggaaataa
    33601 tgcatatgat tgaaatacta taccaaaacc tctctttgat ggctctaaat ttgtaatatc
    33661 tttatcctgg agaattatag ttccgctatt tacatcttca agacctgcta ttattctaag
    33721 taatgtagtt ttaccacatc cacttggata ttattagtta gtccaaataa ataaagtact
    33781 tataacaatt gaaataagcg atatacaatc aaatgttagt ccacaatact attctaactt
    33841 accttgacct ttaaatactc tgttcctttt taggaataaa atattattct tatttataaa
    33901 tttattcaat tttcaaacgt atatgctgag taataactag ttatattgca ttgctttagt
    33961 tgtagaataa aactaaatcg gcaaaactag agaaatttag tgacgcaaag ctatagggac
    34021 taagacttat aaaattctta taaattatgt cagccagttg ccaaaaagat attgttcttt
    34081 ttgtttttat gaaagttttt tagggggata atatatatac ttagagataa aataaaatat
    34141 atttctagca ttcacagttt tattttttct taaaatgcga tataataaag atgtaaattc
    34201 gtatcaaaaa taaaaaaaga aactacaatc tattagccta gagcgaagtt tcaaattcaa
    34261 aataatttaa gtaatgattt attcattgaa atcgtcttga tggcagtcag gacgatttct
    34321 tagtttatgt aaagattctt ttaatttact tatagaatct aataatcttg ttatagactt
    34381 cactattgtt gtaatagatt ttattataat agtaatataa tacgctataa caagtataaa
    34441 cataagtgtt ttcatatact cacccccttt catacgaagg tgatttctga aaactttcgc
    34501 ccttagattg tagtttctta caagatatat tcttgcattt taattataac ataattttac
    34561 aattatcaaa tatatattct tcatattttt tatttataca atatattcta tattgcaata
    34621 aaaaacattg ttttaaatat atatatacat aggtatatac taaatatgtt aaacttaatt
    34681 atggaaaata taaaatgaaa ggaataaaaa tatgactaag actatattat gtgattactg
    34741 taataaagga ataaataaag atgataataa gtatattact tttcataaga aaagtcatat
    34801 gaaaactaac atttgtatta attgtgcatt aaatttgata gataaagata aattaaatga
    34861 aaatattata aataatcaac atggttattc aaagaaatga aaaaagcact ctccataatg
    34921 aagaatgctc tatataataa tgtttgactc agtaaagatg cattggggtt acatatttac
    34981 ttttttatta tatcatataa tttactttat gaaaaagacc tatttaaatc aattttaagg
    35041 tgtgttgagt aatgttcttg atagtttata tgttgatgaa tttcaaaaaa ataagcactc
    35101 atataaaaga gtgctttaaa tgtatattta tgaaatatgc aatataatta tagcatgtct
    35161 tatgttttag tatgataatt ttcgttcgtt ttattattac aatatgtatt atagtttttt
    35221 aactaataaa aaattatgtt tggaaattag aaagatgcta gaattaacct accatctttt
    35281 tatctaatgt tgtattattt tattgtattt tgtaagctca tattttacca atctgagcat
    35341 aattttattt tcgagttgat atatatccaa tatttctata aaattatatt ctccatttaa
    35401 ataatcttgt aaatttaaaa tcaaattaac ataagtacaa ctaccttagt tatatcatat
    35461 atttaagttc tctttatatt aaaaatgtaa gtaaagtgta aataaacatc ttaattttag
    35521 attatgaacc tatttatcta taaaatccaa tgccttgtaa aaggtatcaa acctatcaat
    35581 attcttatta ttatgtactt ttctttagtt atgcaattaa taaatataac cagttttaca
    35641 attataatgc ctagttttgc ataactactt gtaaaatatt aaattttgaa gtattgttta
    35701 gataagattt tagataatgt aatatgcata atttactgaa tattctttgt ttttcgtttt
    35761 ttagggggat tgattgtgtc taaagatatg attgatgaat gtgttcatat aatgactgca
    35821 tatattacta gtttaaaaga atattattca tttatagaaa ctcaaattga tgattttatt
    35881 aaaaaatatg gagaagatat tgtagaatct tgccttcata gaataatgat tttattatgt
    35941 gaatgtggtt tagtataaaa ttcaatttat taaaaaagat aagaagaacc ttatataaac
    36001 tcttcttatc tttatatcta ttttattaaa ttatatgttt tataattttt cttttacaaa
    36061 ttctatagct ttatccattg ttgaccatac atcattacca tatagttgag taaatttttc
    36121 tcctgtagtc ttactcattt ccttcatttt attacaagtt actccaccaa ttacatacaa
    36181 attttgtgtt ctatgtgctt tatagtcttt tatatctgta actaaataac tttctttttc
    36241 tctcttataa tataatccta aaatgtctgc
//
LOCUS       DAEMHW010000017        34864 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEMHW010000017 DAEMHW010000000
VERSION     DAEMHW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34864
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.017"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            97..1425
                     /locus_tag="KPK67_000737"
     CDS             97..1425
                     /locus_tag="KPK67_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457739.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINPLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(1553..2743)
                     /locus_tag="KPK67_000738"
     CDS             complement(1553..2743)
                     /locus_tag="KPK67_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2457740.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNIGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEVLLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(2849..4228)
                     /locus_tag="KPK67_000739"
     CDS             complement(2849..4228)
                     /locus_tag="KPK67_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2457741.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMRYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(4259..5455)
                     /locus_tag="KPK67_000740"
     CDS             complement(4259..5455)
                     /locus_tag="KPK67_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457742.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQKFFG
                     IPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAKLSFV
                     AMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNGIIMF
                     GCMLMLPGGLAKVTPTALFGMIIPTIGILVVGSVFIILFTAIVGKLLGYSPRMSAAIG
                     VTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAFASII
                     GPIIFG"
     gene            complement(6480..7934)
                     /locus_tag="KPK67_000741"
     CDS             complement(6480..7934)
                     /locus_tag="KPK67_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG2457743.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIKNNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            complement(7954..9825)
                     /locus_tag="KPK67_000742"
     CDS             complement(7954..9825)
                     /locus_tag="KPK67_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2457744.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASMLALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            complement(9815..10682)
                     /locus_tag="KPK67_000743"
                     /pseudo
     CDS             complement(9815..10682)
                     /locus_tag="KPK67_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(11277..11681)
                     /locus_tag="KPK67_000744"
     CDS             complement(11277..11681)
                     /locus_tag="KPK67_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG2457745.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNYIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
     gene            complement(11873..13060)
                     /locus_tag="KPK67_000745"
     CDS             complement(11873..13060)
                     /locus_tag="KPK67_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBG2457746.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTNTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            complement(13092..13517)
                     /locus_tag="KPK67_000746"
     CDS             complement(13092..13517)
                     /locus_tag="KPK67_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2457747.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIC
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            13996..14745
                     /locus_tag="KPK67_000747"
     CDS             13996..14745
                     /locus_tag="KPK67_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG2457748.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLEGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(14911..15276)
                     /locus_tag="KPK67_000748"
     CDS             complement(14911..15276)
                     /locus_tag="KPK67_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2457749.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWRSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(15292..16068)
                     /locus_tag="KPK67_000749"
     CDS             complement(15292..16068)
                     /locus_tag="KPK67_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2457750.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            complement(16479..17906)
                     /gene="ascB"
                     /locus_tag="KPK67_000750"
     CDS             complement(16479..17906)
                     /gene="ascB"
                     /locus_tag="KPK67_000750"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG2457751.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            complement(17967..19895)
                     /locus_tag="KPK67_000751"
     CDS             complement(17967..19895)
                     /locus_tag="KPK67_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2457752.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            complement(20040..20882)
                     /locus_tag="KPK67_000752"
     CDS             complement(20040..20882)
                     /locus_tag="KPK67_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2457753.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFYEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            complement(21188..22588)
                     /locus_tag="KPK67_000753"
     CDS             complement(21188..22588)
                     /locus_tag="KPK67_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2457754.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKEINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFPAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKESTDNVVDRV"
     gene            complement(22627..23298)
                     /gene="pgmB"
                     /locus_tag="KPK67_000754"
     CDS             complement(22627..23298)
                     /gene="pgmB"
                     /locus_tag="KPK67_000754"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG2457755.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            complement(23300..25690)
                     /locus_tag="KPK67_000755"
     CDS             complement(23300..25690)
                     /locus_tag="KPK67_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG2457756.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDYFD
                     LSKGITSEHTRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMKKLNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETHNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNVGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKHNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NATQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            complement(25716..27164)
                     /locus_tag="KPK67_000756"
     CDS             complement(25716..27164)
                     /locus_tag="KPK67_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG2457757.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            complement(27569..28456)
                     /locus_tag="KPK67_000757"
     CDS             complement(27569..28456)
                     /locus_tag="KPK67_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2457758.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            complement(28556..29005)
                     /locus_tag="KPK67_000758"
     CDS             complement(28556..29005)
                     /locus_tag="KPK67_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2457759.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            complement(28995..30902)
                     /locus_tag="KPK67_000759"
     CDS             complement(28995..30902)
                     /locus_tag="KPK67_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2457760.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            complement(31170..33065)
                     /locus_tag="KPK67_000760"
     CDS             complement(31170..33065)
                     /locus_tag="KPK67_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2457761.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKKLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQYWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(33101..33979)
                     /locus_tag="KPK67_000761"
     CDS             complement(33101..33979)
                     /locus_tag="KPK67_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBG2457762.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILKAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(34522..34692)
                     /locus_tag="KPK67_000762"
     CDS             complement(34522..34692)
                     /locus_tag="KPK67_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457763.1"
                     /translation="MSALLYGVCNKPISVSIVINMQEFKIGVYMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
BASE COUNT        11389 a         6140 c         3717 g        13618 t
ORIGIN      
        1 taattgaatt taattgaaat acgattttaa ctagaacaat agattaatgt aatatttaat
       61 ataaaataat tataataatt gaaaaggaag taatctatga tgaaaatagc agtcataaca
      121 accgaatttt taaaagagtt tgtagataat tctataaaaa agttaaatat taatgcagaa
      181 attgaaatat atatctatag agatttttct catgttggag atttatattt ggaaatagaa
      241 gataggtttg atggatttgc agttagtggc cctatcccca aaaaagctat tacaaaaaag
      301 gctggaacta ttaaaaaacc tctagttgat tttggtactg acttacaaag ttactatgat
      361 atgtttctaa agcttatctt taaatataat acattagatt tttctagagc atactttgac
      421 ttaacagact gggcagataa tccaaaagat atatcatatt atttatcaaa tggcacattt
      481 ggagacttaa tggatagtat tgatgaaaat gcatctaaaa tgagtttatc tgacatagca
      541 aaacttgaaa aaaagattgc aaaaaaacat attaatctat ggaatgaagg gaaaatcgat
      601 ttttctacta ctcgttttag tagtattgtt cctatgttac aaaatgcagg agtaaacttt
      661 catttcatat accctgatat agatttatta aaaaatactt ttaataactt aataaaagat
      721 attcatttac atagaatgaa gggaaatcaa ccaagtgtaa tatatataac agttaaagat
      781 acaaattatg atgactctac tctagaccaa gtttataaaa tattaaatag tattaaaaaa
      841 gataaattaa ccgattttat aattgaaaaa aatagtaatt cttttaaaat tttcactaaa
      901 tgtgaaacga ttaaatcatt aactaaaaat tttactgcat gttatataaa agaatctatt
      961 gaaaatagta caaatattaa agtatgtgtt ggatatggaa ttggtaatga tataaagcaa
     1021 gctatgtcta gtgccataag tgctaataag gaatctaaaa taaatccttt aaattacagt
     1081 ttcttagtta acgaaaatta tgagacaata ggacctttac tgtgtgataa gttacttact
     1141 gtttcaaata aagtaactcc acatataaat acaattgcca aaagttctaa attgtctact
     1201 cttactattc aaaagattat atctgttact gaaattctac aaagagatga agttactcct
     1261 aaggaattat cttcatgttt aaatgtaaca gttagaactg ctaatagatt tctaagtagt
     1321 ctcttaaagt ctggaaacgc tactatatta tatgaaaaac aaaactcaac aaaaggtaga
     1381 cctgaaaggg tatacaaatt atttatatca gaaaataaat attaaaatcc aaaatgcata
     1441 aaaatatttt atcatctaat attaagtaaa attatatatg actttctaaa aggtatatct
     1501 ttgcatcttt ttaagatata ccttttatga tatttaataa tattttatgt tattatctac
     1561 taaaattatt ttctaaccat tttactgcac aatgagcaaa taaagctgaa ccataaacta
     1621 atccctcttc attaaaaatt gctttaggat tatgcatacc aaaattatat ccctgactaa
     1681 tacttcccat tccaatccat aaaaatactc ctggaaccct tagtaaaacc tctgaaaaat
     1741 cttctgaacc cattgcagat ggaaagtcta ttacattttc atttcctata acttctttaa
     1801 cataagatac aatctcatct cctacatcca aatcatttat aactgcagga gtccccattt
     1861 taaactccac tttagcattt cctccaaatg atttagctat tcctgtagat atttcaataa
     1921 ttctattttt taatttttct ctaacaaatt ggtcaaatgt tcttaaagtt cctgacatag
     1981 atgctttatt tggtatagca tttggtacgt ttcctgcatt aaacatacca atagttaaaa
     2041 ctacaggttc tttctgaggt ttttctcttg aaagtatagt gtttaatgct atttgtatat
     2101 gcgacattat gtttattggg tccactccaa attcagggct tgctccatga gttgcttttc
     2161 cttctactgt tatttcaaat atatccgcag atgccatagc tggaccacgt gaataagcta
     2221 cttgacctat gttaagaaat ttacccacca tactgtgaaa tgccatagct gcatcgacct
     2281 gaggattttc taatatacca tcatcaatca ttgattttgc acctctggta gtctcttcat
     2341 ctggttcaaa tacaaacttt actctacctt taagtaagtg cttattatcc ataagtattt
     2401 tagctgctcc taagagcatt gctgtgtgat tatcgtgtcc acaactatga gatgcatctt
     2461 tattcttact tttaaaaggt aaatccgtct cctccataat aggaagtgca tccatatctg
     2521 cccttaataa taaggtcttt ccaccatttt cactgtctaa aacagctact attccattat
     2581 tagacgtcaa tttcggagaa tatcccattt tttctaaagt actggtaaca aaggttactg
     2641 ttttcggtag attgcaacca atttcgggat tttcatgtaa ataccttctc catctaataa
     2701 tttccccttc tattgactgc gcttctttaa aaaaattttt catattcctt cctcctaatc
     2761 agatagtcag caaaatctca ctttctttta gtaatgaaat gatgctgata taaaaattat
     2821 aattttatat attaaatttc atgtcttttt aaggagttgg aatatcatct ggaataggac
     2881 atttatatgt tttaccattc atagatttat caaattcttc ttttgccttt ttagctatct
     2941 ctggattatt taatatttta gctccaaaca tagccatgat ttttgatgca tatctcatac
     3001 ctttttctcc tatagaactt cctgcacaag atgtaaactg ccagctatga cctggtgctc
     3061 ctagattcgt acaccctgta aaaaagtatg ctgtcggaac tatatgcatt acatctccaa
     3121 tatcagtaga gccataacta ttaaagtttg taacatttcc tccaccataa tatagatgag
     3181 ttccttctgg caaattatat ttacttatca tagccttata ttgttgtggg ctttgtctat
     3241 ctaattcagc tgcaaaatcc aattcctctc gagtccaagg ctcttgttta atctcattca
     3301 tacattctga tacaagattt gctaaaacat gattgtttaa agtattataa catcctccta
     3361 aaaactctac ttcaacttca gtttcagtca tcattgcagc tccttttgct acctttacaa
     3421 gtctttcata tgtactttct acagcttctc tacttaaagc tcttgtataa taccatactg
     3481 atgctttatc aggaactatg ttgggtgcta ctcctccatc tgtaattgta taatgaatcc
     3541 ttacatcaga agtaacatgt tctctcaaat aattagctcc cacattagta agttccacag
     3601 catctagtgc acttcttcca ttttgagggt ctgagcctgc atgtgcagtc tttcccttaa
     3661 agtgaaactt tacagagtta acagctgttg aagtaccaat tgtagcttca ctaattttat
     3721 ttggatgaaa atttatagct aagtctaacc cttcaaacgc tccacctcta gccataaatc
     3781 ctttacctgt taattgttct tctcctggac atgcataaaa tactatagtc ccacttaagt
     3841 tattttctat catctctctt ttaagcccta ctactgcccc tacatgtgct gtacataata
     3901 aattatgccc acatccatga ccatatgcac ccaattcaaa tgcttcttgc tttgttgaaa
     3961 ctttttgatt taaaccaggt aatgcatcaa attctcccat aaaacctata actggtttgc
     4021 ctgaaccaaa actagctcta atagccgtgg ctaaacctcc tacacctact tccacatcaa
     4081 atccttcact tcttagaaaa tttgctgtat tttcacatgc tttaaattct ttaaatgctt
     4141 tttctggatt ttcccatatt ttcttactta aatcatcaag tttgaatctt tttttatcta
     4201 tctcatttat agatatactt cctatcattt cctattcctc ctataagtat atttactatc
     4261 aaccaaatat aattggacct attatactag caaatgccac tgatgcaatg gttacagtaa
     4321 caaatcctcc tataatcatc tttggtaata aataatctgt tgccctttgt ttttcttctt
     4381 cagttccttc catagcatca ataccttcgt tggaaattat ctgtgttgca ggataagcta
     4441 gcatacaagt tacaccaatt gcagctgaca ttcttggaga atatcccaat agttttccta
     4501 caattgcagt aaatagtatt ataaatacag agcctacaac aagtattcct atagttggaa
     4561 taatcatccc aaacaatgct gttggagtta ccttagcaag tcctccaggt aacatcaaca
     4621 tacaaccaaa cattataatt ccattagatt gagatttatc taaaattcct ttttctaaaa
     4681 aacctattcg tgtaaaaatc aaccctacta acaaacatgc tatgttggga tttaaataat
     4741 agtttgcagg ggtaaatcct ggtattaaag tagccaatcc tacaaaatta ccaatcatag
     4801 caacaaatga aagctttgca agatacacta aagtagatga ttttttacct aaatcttctt
     4861 taaaaattct catacttgga agtttaaaat tactttttga ctcatctttc aaaaaatccc
     4921 cattatctct tttaaatttt aattctttat ttatacaata tgtagttatc ggaatgccaa
     4981 agaacttttg aaatgataat actagaatta caaatgcagc aagttcaggt ctatttgcag
     5041 cttctgcaac agttgttacg ataataccag caacagtaga acctgctatt ggaggtgctg
     5101 ctattaaagc atactctcta ccaaataaca tactaccgcc aacccctatt cctacaatac
     5161 ctactaatgc aattacaacc gttttccatt catgacataa gtcttcaaga tttataagtg
     5221 ttcctaagtt agttattaat aaagctatac caaagttgct cattgcagct aatatccctg
     5281 atgctcctgt aatgtctttt ggtaaaatat ttgtccaaaa tcctagtaaa aatataatac
     5341 atacaaataa caatgttgaa attgcacctt ttgttttctt agataaaaac tcactaaaag
     5401 cataaatcat tacacataca gtaaagcaag taagtggatt ccaaaaattt tccattgttg
     5461 tttcctccta aatataataa ttttttatca ataaaacaat aattataaaa tgtatattat
     5521 gcagttattc ttaactacat atggtatagc ttgatatttt ttgatgatat tatatacgtc
     5581 ctaaattatt cctaaaatat ctaaataaat ataactagag ttaactctct aaatattaac
     5641 tatagttatc aacttactag atttcttttt gatttttact ttctacataa ttttacgagt
     5701 tagaattgta cttcaactac tctctatatt agtatacatg ttggaagact tatatttatt
     5761 taaatattag atatagaata catttttata aacgtcttaa attattctta aaatatctaa
     5821 aacgaaataa taatataccc attaaaccaa tattatcaga atatacttca ctttaagtct
     5881 tctttatata gaaattttat ctattagtgc atcccctcct ttgtaaatta gactatttct
     5941 aaacgaattt agatatctta ttcctaggaa ctattactag aaacaaaaat tatttattat
     6001 attaaatcaa tgataagcca tactttaaaa ttctttctat acaagtatga tttatcatat
     6061 aatgacatct atacctttat ctttatagta gtcctaaatt attcttaaat tgacaataaa
     6121 aataatttca ctatttgtaa aaatacttgt ttttatattg tcaaactatt cttaatatag
     6181 attataattt taaatctttt tttcgtcaat atattctgac atttttccag aaaatttata
     6241 aagttatata gtgagtaaat catactattt ttatataatg agtaaattat actatgaata
     6301 tgtctaaata ttgacttaag tataaacata aaaaatgtgt attctccttt ttatagtaat
     6361 tttagttgcc attactttta ataagaatac acactttact ttaaagaccc tatactctat
     6421 tttaaatgct ttactagaca ctaattaaca ttagttccta acttctattt atcttattcc
     6481 taccctacta ctatatccgt agaaaaatct aaaacttctc cattagattt tattacttct
     6541 ttgtaccaat caaatgattt tttcttatat ctcttaaaac ttcctttgcc ttcatcatct
     6601 aagtcaacat agataaatcc atatctctta ctcatctgac ctgtagttgc actgactaaa
     6661 tcaattggtc cccacatcaa atatccaaaa cattcaacat tatcctcttc aattgcagac
     6721 ttcatttgag atagatgctt acttaaatat tcaattctat aatcatcatt tatagttcca
     6781 tcttcttcga ttttatcata tgcccctaaa ccattttctg taattaagat aggtaattga
     6841 tactttctgt aaatatagtt caaggtaaat cttaaaccta tagggtctat tgcccacccc
     6901 cacttacttt gttccaaata tggattttga atcccaccaa ataaagattt atcacctgat
     6961 aaattttctt ttggagtaga tacacttgaa aaataataat taagtcctat aaaatctaac
     7021 ttaccttctt taaatgcttt tttgtcttca tcagtcactt caatagttat attatttttt
     7081 tcatattctt ttaatttata ttgtggaaac ttcccattgc acattgcatc aatttgataa
     7141 taatcacggt cattatctaa aaatgcattt aacacattac taggattaca attgtatgga
     7201 taaattggat ttagtccaaa tacacatcca actttattgt tgggattgat ttctctagcc
     7261 aactttgcaa cctttacacc cgccaaagtc atattatacc ctacatttgc caatgtttga
     7321 accttatttt ctatttcact atacttaata cctgctatca tataagtaaa aatatccgag
     7381 tgctctgttt gagggtcaat atggttcatc tcattaaatg taacccagta ctttacctta
     7441 tcatcaaatc ttcttattac agtctcactg tactttaaat acaaatctat aagttttctg
     7501 ttattccatg aaccatattt atgtactaaa ttcataggta attcaaaatg atatagtgtt
     7561 acaataggtt ctatattatt ttttattaat gtatctatca gaccttcata gtatttaatc
     7621 cctaattcat taggattttc atcatctcca tttggaaaaa ttcttgacca atctatagat
     7681 attcttaatg cactaaatcc catttcttta aataaagcta tatcttcttt atatctatta
     7741 taaaaatcta ttccattatg agatggataa tccaattttt cttctatttt atcagtaatt
     7801 actcttggcg tttcataaga gcccttagtt acaaaatcca taattgcagg tcctttgtta
     7861 tcactatccc atgaaccttc acattgatgg gctgcaatac ttcctcccca gaaaaatgta
     7921 tctttaatat tcatgaattt tccctcctaa aatttatact attacagtta ataattcttc
     7981 tgattcatca tttgatgaga ttacatctaa ataatcgcta gtatttgtaa ctacagttat
     8041 tatagtagaa tcaaagcctt cctctataat tttatcagat tcaaatactg ctaaagcatc
     8101 tcctttttta accctttgat tttgtgttac aagactttca aagaattttc catctaagtt
     8161 tacagtatct atcccaatat gaattaatat ttctaatcct tcatcagtca ctataccata
     8221 tgcatgttta gttggaaata ctatagttat agttccatct acaggagata taacagttga
     8281 ttttgattca ctaggtataa ctccaacccc tttacctaat gctccagagg aaaatgcttt
     8341 gtcattaact tccccaagtg caattacctg accttttgca acatttccaa tactaatagt
     8401 tcttgaattg ttctcgttga ttattagttc taattctttt tcattatcac ccttaacttc
     8461 ttctttttca tctgaaggta ttcctagtaa ataggaagct gctgttgctg ctacaaatga
     8521 tactactaca cttattatca taaatgtaaa gttagatacc tctcctccaa caagatacgt
     8581 tggtaatgca gctaatcccc aaaccattcc atatgtttta acttgagcta aacctgctat
     8641 taaaccacct atacctccac ctatcattac tgctacaaat ggtcttctat atttcataaa
     8701 cacaccaaac actgaaggtt ctgtaactcc cataagtgca ctaaaactta ctgtaccaaa
     8761 tagttgtttt tgttctttat tcctagtttt taagaaatat ccaaacattg caccacaaac
     8821 tgcaatatca gatattgttg cagcgcccat aaaaattggg tcatacccca atgttgtcac
     8881 agaatttaaa gatataggca tagtaaagtt tgctgcacca aaaactatta agagtggttg
     8941 taaagctgca tacagtgcaa caacactcca attacccaat gtatcaatta aaaacataca
     9001 tgcatagttt aatccatcac caatccacat accaataggt ccaaatacag ttaatgtaac
     9061 tgatagagtt attattaaag ataacactgg aactaaaaaa tagtgtagtg ctttaggtaa
     9121 tatttttttc aaaccttcaa ttataattcc cattgcccat actcctaaaa gtattgggat
     9181 aaaactgttt gcataagttg tagcctgtaa atttatacca aataaactta atccttctac
     9241 accatctatt gatgaagata ataacgtaac tgctaacact actgctatat agggatttac
     9301 attaagtctt tttgctgatg ctattgcaat taatactggt aagaagtgaa atgtagcact
     9361 tcttaatgta tctaatattt gatatgtagg gctatcactc tgaagtatgc cagacatatt
     9421 taaaagtgct aacatacttg cgagtatacc agctgcaagt agtacttcaa ttactgaagt
     9481 catagtctct gagactacca caaagatgga attaaatata cctttgatac ctctaattcc
     9541 cttttttctt tcacttttta tacttgattc acttgaactt aaacctatca tagacataaa
     9601 ctcgttatat acatctacaa catgagtacc tattataatt tgataagcaa catcattaga
     9661 aactacatca attactcctt ttaatttttt aatttcttct gtttgagcga tactcctatc
     9721 atttaaagta aatcttaaac gagtaacaca atgcgctacc gactttatat tttcttttcc
     9781 ccctataagt tttattatat tttcatttaa atctttatat ttcatctgat tttgcctcca
     9841 gtctttgagt caccctattt atatgcaaca ttaaataagt ttcttcactt atagatatat
     9901 cgacattaaa ttgtgatttt tgattttata tattttctta tcttttgaac acttccaaat
     9961 gactttggat aaagttgact tacttgttta tataaatcta tagaattatc ttcatctatt
    10021 gaagcttcat ctgacataat tctttggata aagtactgta gatgagtagt aaatctcata
    10081 taatttgttg aagtttcatc tagctctaca ttaaaatgat attttattat agaaactata
    10141 tctgctaaag ttctcaactc cacaattgta ctatcatgag actctttatt tacctccata
    10201 tttacaaaat gaagtgctat tgatactgcc tcatcatcag gaagattaat tcccttacta
    10261 tcaccaatta attttagtgc atgcattcca atttcaaaat gctttggata aaatctttta
    10321 acattccata ccaaaggact ttttataaat tgattctttt cacatctttt aagtaaaaac
    10381 tctaaatggt ctagtaaggt taagcttaga taatcacttg actttatatt taaagctttc
    10441 tcaccatatt caattatttc attaataaga actatattat tggagtctga gttttgtagt
    10501 agataactaa agtgttctag catatcgtat gtatctagta tatatattct ctctatttca
    10561 ttttcattta ttacttgtcc cactcttttt ttaaagccaa ttcctttaac aaatacaata
    10621 atttcatttt tacctctttt tactaaggct acattattat ttaaaatctg aattattttc
    10681 atatattcct cctttcaata aaaagaactc aagcatatat gtatagagta tccctctatt
    10741 tttcacatat atacttgagt tctgcctgct aatcagtaac atactcaaaa ttatgactac
    10801 gtcgccataa ttaaaattct tttttgttta atttcatatt ctatatgtta tactatccat
    10861 ataaatctgt caacggtttc ctagatattt ttttattcta taactacaag ctctgttatg
    10921 cgattatctg acaaggttta tatactaaat tgatactatt ttaccgtcac taatatttat
    10981 aatattgtct gaaccattat ctattttact attatgaatt atcatagtaa tgcttttaaa
    11041 tataatgaac tgtatgttaa caaataaatt cttaagatat attaacatat aaagtcttaa
    11101 gtttagtttt atttaatcac tattaagata tattaatagg taaagcttca aatttagttt
    11161 catttaaatg ctagtaagat atactaatat ataaatactt aaatttagtt ttatttaaac
    11221 gctctagtga tatttttaaa taaattttaa taaaaatcat tctattttgt cataaatcat
    11281 ttatctggct tatatttaag atatattggt ggatacttta cactttttaa ttcctcatta
    11341 cttcttaaaa gtttattatt attttccata tcataaattt gtaacattac tttatttaca
    11401 tatagagcat aataattatc actataaaaa tattgatact catatcgagg gtcatctttg
    11461 taatacactt tgtatagtat tccttcgtaa cttgccttct tggctttttc ttttgtaata
    11521 ctttttatat tatcttttga aatatcttgt tttaccatat acttatcaat atatgtttca
    11581 gcatctttcc atggtttaat gtagttttta tatacaacaa accctgttac acttataata
    11641 cctataatta atactataaa aataacttta tattttttca tattaatcct ccttcattta
    11701 ttatttaaaa cttgatataa tgcactaaaa aaaggatgtt atctttttta ttaaaatata
    11761 acatccctca tatttagcat caatatatat gccccaactg gtaggttaaa agaccccact
    11821 gtcagtactt tgattaaatt acatgtatgt atatatattt ttaacaaatc tattatatgt
    11881 caacttgtct actattacat ataatctgta atattacatc tgctgctttt tttgagcctc
    11941 ctgcattata aaagcttttt gatagtttag ttgcattttc tttatattca ttgtcattta
    12001 taacttgaaa tactgctttt ttaatacttt ctgacttatc ctcttttaac ataattcctg
    12061 ctcccaaatg aacaactctc ttagctacca ttctctgctc cgaatgttga ggaaataata
    12121 ccataggtac tccataatat aaactttcat taacactatt catcccagaa tgagtaacaa
    12181 acacatcagt tttttgaagt atagcaatct gttcaacact atttttaacc tcaaaattat
    12241 ttggtatatt tcctaaactt ttaatatcag tacttctacc aacagacata atcacatcaa
    12301 catcagaatc tttaaatgca tttatacagt ttttataaaa gctaacattt ttattattaa
    12361 ttgtaccaag cgaaatatat attagctttc tttttctctc tttaggttca ataatcaact
    12421 ttgatactga tggacctaca aatgaatact tactagagaa agtttctacc attggttgaa
    12481 attcttttga tgtatataca atggtattcg tatcattatc attagaaact atagaaataa
    12541 aattttttac ttcataccct tttctttgta aaagctttat ttttttatta atgcgtctta
    12601 caccaaaaat catacgtata atttctataa agttttgttt catcaacttt gctgtatgtt
    12661 tattaaatgc aaaagttgtt gtagagcata catatggaat attaagtttt tgtgcaaata
    12721 actttcccca aaaacttaat gaatctgata caatgcaatc tggattaaaa tctttaagtt
    12781 ccctacaaac tttttcatct aaagaaatag tagtatctac tatcatctca attaatccag
    12841 gaaaatcctt accaattttt ttctcatctc cagggagtaa ttctggaaga tatttatcac
    12901 aacaaataaa ttttgctcca gcaccttcaa ttttatcttt aaattcatta aaagaataat
    12961 acaatacctc atttcctcta tccactaatt cacgaactac ttcaatagta ggattagtat
    13021 gaccatatgc aggaatgcta aaaaatacta ttttactcat atttcatacc tccttttata
    13081 attttttaaa atcattcttc tttacgtact actcccacat gacctataaa atttattaaa
    13141 tcacgttgat gaacaattcc taatcctctt tcctcatctc caagtaccaa taatttatct
    13201 cctgcattta tattaaatac ttctcttgct tcttttggaa tgacaatctg accacgttca
    13261 ccaactacaa ctgctccaaa aaaaaacttt cctttaggag gtactataat acctgttttt
    13321 tcttccgaat gattaactaa gtcatctagt ttgacattgt acaattttgc aagtttaatg
    13381 caactacata tatcaggaat agattctcca ctttcccatt ttgctattga ttgtctagaa
    13441 acattaagtt tttcagctaa ttcttcttgt gtatactggt tgatttttct tagacttttt
    13501 aaattctcat taatcatatt tttcatacct cctatgttta taatattgga tttattattt
    13561 aaattctatc aactaatctt aacattcata tatatattta tatactttta ttatataaat
    13621 ttattgttca ggtaacatat ttccttaagt tatcataaac aaaaaaaaca aacacaaatc
    13681 tactaactgt tgttaaagta tttaagttaa ggtcatttac attatcaaat atttctacac
    13741 ctttaaaata acctttatat ttataatctt tcataagcaa attattatcc cttctcatat
    13801 acaagtccag ttgatgtctt gttgctaaat ttaatggtaa tcaaattctt taccaataag
    13861 tataaaaatt actaatgtgt tataattttc ttaactacta aattttacat acatttgaat
    13921 agaatatatc aataaattac tataataaat aaaaaattaa gagttaaaat aaaaaatagt
    13981 tagggagata ttattatgat tttatatttt agcggaactg gtaatagccg atatgtggct
    14041 cgtaaaattg cacaagaatt aaatgatgaa ctgatatcac taaatcaact tataaaagag
    14101 gaaaaaacag atgaattaat atcagcagat aaaccattca tatttgtatg tcctacttat
    14161 gcatggagat tgccaatagt ggttacggat tttataaaag aaacaaaatt tctaggaagc
    14221 aacagagttt actttgtaat gacttgtggt ggagatacag caaaatccat aaactatatt
    14281 caaaaactat gtaaatataa ggaatggcaa ttaaaaggta tggcagaaat taaaatgcca
    14341 gagaattata tagctttatt ttctacaaca gacaaagaaa catcaaaaca aatgattaaa
    14401 gaagcagata aacaaatata taggattatt tctgatatac gcaatgaaaa tgaatttgaa
    14461 actattactc ctagtggctt agaaggtaca attaaaagtg gtataataaa tacagttttt
    14521 tataagacaa ttattagtgc caaaggattt cattatactg ataaatgcat tggctgtgga
    14581 aaatgtgtag agctatgtcc tttaaataat attaatttaa aaaacaaaaa accagtttgg
    14641 aaaaataact gtactcattg tatggcatgt atttgtggtt gccccactga agctattgag
    14701 tataaaaata aaactcaaaa cagagaaaga tactatttag aataatatat tcttaatatt
    14761 taaaccatta taaaatgaac tatacccatt gttttataca gttttacata ttttaactgt
    14821 ctcctaagat gggtatagtt caaaagatag tgtctcaaag tgaacttttt agttcctaaa
    14881 tcaatctttt tgtattaaat caagtataat ttaatcacct tttcttatat cattttcact
    14941 aatatgttgt attccaacaa atataagatt tattagctct gttagtgacc tccattgtga
    15001 tttttttgaa tcaagataat aataattttt tgttccttct ctacgcaaat taacaatctc
    15061 tgcttctttt aatatcttaa gatgatgaga tacagcagga cgagaaagat gtgttttttt
    15121 tgtgatttct cccactcgta taccattaaa atcactctct aagagagcaa ttaaaatcaa
    15181 ttgtctagtc tcatcaccca tagcagagat agctttgcga cattctttaa atttagacgt
    15241 tatttcttgc attctttgtt gacattcttt ttccataaaa tccaccctct cttataagta
    15301 tctaacagtt gctacttctt tctgtgcagt acgtctatat ttaacatctg ggtatccaat
    15361 tactaatgtt gtaagaacat gattactgcg ttttagacct aaaagctttt ttaattttgg
    15421 cgaattattt gctacatctg aaaaatatcc actaaataaa acacctagac cataagactc
    15481 tgccattaat gccatatttg atgctgcaag tgaaccacta atcttatctt ttgtaattat
    15541 cattattgca ataggagcat gcttaaagaa aaagttatcg tcaatctcaa cttcttttga
    15601 atatttaata gctatatttg caataggttt tatttttcta aaaaatttca ctgcttcgtt
    15661 ttcataaatt gactttttat tgtctaaaac aatatatgat acatcttgtg aatttttagc
    15721 actaggggta tacctacctg cctcaataat ttgctttata atttctgaag aaacttcttt
    15781 atctttaaat ttacgtatac ttcttctaga tttaattgcc attagtaatt catctgaatc
    15841 tagctttggc ttatttgtaa gttctatagg aggttcatca aatcctgtca aggtaatagc
    15901 ttttgtggga catattgctg cacaatgtcc acacattaga caatcttgtt ttttaattac
    15961 tgacttttta ttttctataa ttatattgtt tactggacaa tcatttttac aaagcccaca
    16021 tccaatgcaa agttctttgt caacttctat tatatgttgg tatttcatat tcattcatct
    16081 acctttcgtt tattagttta aactattata acacgtagac attttttata ttcattggta
    16141 aagaatttga ttaccattaa atttttaatt tctctattaa ataaattttt catagaattt
    16201 taaaatatca tagtttatac ataataaaaa aggcaagctt aaaacctaag cttgcctttt
    16261 tcatctttta ttacaaagta aattgcactt acttcccata cttcttaaca attttatttt
    16321 ctaaaatttt atttgtgaat catactattg tataaataca atgtaaaata attctgtatt
    16381 gattttctat agttaaaaaa gttaaaaatt atacttatta ttaattttta tactcttctt
    16441 atttaataca aagttcttat ttaacataga tttcttgtct aatataaatc ttcaccattt
    16501 gaagcaatta ctttcttata ccattcaaaa cttttctttt tcattcttct tagacttcca
    16561 ttaccactat tgtctctatc tacatatata aatccataac gcttttccat ttcacctgta
    16621 ccagcactta ctaaatcaat aggtgcccat gtagtataac ctaataaatc gaccccatct
    16681 atagaaactg catcatgcat agcttttata tgttctctta gataatcaat tctgtaatca
    16741 tcttctacat aaccattttc atcagctata tctttagcac ctaatccatt ttctacaata
    16801 aatagtggct tttcatatct atcatataca tgatttagtg taattctcaa acctaatggg
    16861 tcaattggcc atccccattg actgtatgaa agatatggat ttttagcaga tgtaaatatg
    16921 tttccttcag ccatatcatc tgcattttca tcggttctaa cacaacggct attgtaatat
    16981 gaaaatgaaa taaaatctac tgtatattta ttcaataatt caatatcacc aggttccatt
    17041 tctggtgtaa tacctttttg ctccatatac tttaacgcat agttaggata tttaccttta
    17101 gattgtacat ccacaaagaa gtaattttct ctatctaact ttgtagcttc ccatacatca
    17161 ttaggattac aactataagg atatacactt ccagctgcaa acatacatcc aattaagtta
    17221 tttgaatcaa cttcatgtgc tattttagtt gcaattgcac tagctacaag ctcatgatgg
    17281 gcagcttgat attttatctt ttcaacatct tcaccttctt caaagcaaat accagcaccc
    17341 ataaatggag catgtaaaat catatttatt tcattgaaag ttagccagta cttcactaat
    17401 cctttatatc ttctaaagat aacattgcat aatttctcat agaaacctat catttttcga
    17461 ttacgccatg caccatactt tttaactaaa tgcattggac aatcaaagtg tgtaatagtg
    17521 actaaaggct ctattttata ttttttacac tccttaaata tattttcata aaattgaagt
    17581 cccttttcat ttggtgtttc ttcatcacct tttggaaata ttcgactcca tgcaatgctc
    17641 attctatata ctttaaatcc catttcagca aataacttta tatcttcttt ataattatga
    17701 tacatatcaa taccagtttt agctggataa taatgctcat catcaaaatc aaacattttt
    17761 agtttccctg taataactgc tgttctatct tttcctgttg gacatacatc tacatttgcc
    17821 aatcctcttt cgtcttcatt gtatccacct tcacattgat tggccgcagt agctcctccc
    17881 cataaaaaat cattcttaaa actcatagtc aaagcctcct aaaattataa ccaaagcact
    17941 agattatgct aatacctcaa tctttactaa aataagactg taattagatt gtcttctaat
    18001 tcaatatttt tactttctgt ttctactaca tctaaaaatt gtgatgaatt tgtaataaca
    18061 attggagtta cagtactata ccccaactct tttattgcat caacatcaaa gtttaatata
    18121 gtttgacctt ttgtaattct atcaccttgt tttatacatg cctcaaaacc tcttccctct
    18181 aattgaatag tatttaatcc aatatgaata agcacttcaa cacctgtatc agaaagaatc
    18241 cctatagcat gtagactagg aaataaggtt gtaactaccc catctactgg agcaactact
    18301 tttccatcac taggtattat tgccacccct tgacctagta caccagatgc aaatgctgca
    18361 tcctctatat cacttaattt taatacttca ccttttatag gacttgttac tatttctctt
    18421 tctaatttag tagacttgac ttcttttatt tcttctttac ccttatttct tactttttta
    18481 gactcttcta ctttctcatc tttatataaa atcatagtta atacaaaagc tacgaccatt
    18541 gatatagcta taccagaaag agctactatt aagttaccca ttcccccatc tggttcaatc
    18601 attgctggaa gttcaaatat tcccattcct ccagttataa attttctaag attaaagaat
    18661 ccatagaaag cgcctccaat accactagca atacaactta ttataaatgg tttctttaaa
    18721 ggcaataata ttccataaat tgctggctct gtaactccaa atataccaga tataaagttt
    18781 ggtattgcca tctcttttag ttttttatct ttagtcttga aaaatattgc caacacaact
    18841 gccgaacttg caaatgtaca ggcaaagaat ggcatcataa cattatcata tcctaaagtc
    18901 ataacattgt ttatatatac aggaataaat ccccagtgca taccaaaaat aacaagaatt
    18961 tgccaagtaa accctactat agcacctgcc aatagtggac taaaatctct tattgaaatt
    19021 acaatttgtg aaataagtgt agaaccaaat gttgctatag gacctataac tataagtgca
    19081 acaggcactg ttattaataa tgtaagcatt ggcacaaaga aaaacttaac taaatctgga
    19141 acacacttat taaataattt ttcacaccta gaagcaaaat acactataaa tataattggt
    19201 acgactgttc cagtataatc aattgataca attggtagtc caaagaaatc aatatatact
    19261 ggtgatgcaa acattgttcc tttaaataaa gtgtacatca catctgctcc tgctgatata
    19321 ctgctacctt gtatagcagg ataacatact gcagctccta gtgctaatcc caacattggt
    19381 tttaatccaa acttcttagc tgaagtatat cctaagaaca aaggaaggaa tgtaaataat
    19441 gcatctcctg ctgcatttat taccatataa ccaccagata catcactata taatcccatt
    19501 gctacaaata atgcattaaa tcctttcaac ataccacaag ctgccattat tcctaacact
    19561 ggctgaaaaa ttcctgatat tatatctata cctttatcta gtatattacc tgatttttta
    19621 gtttctgaac ttgtacttga atcatctaaa ccaattattt caactacatc tttatagaca
    19681 gcttcaacat gatttcctat aactacttga tattgtcctc cacttttcat tacagtaaca
    19741 ataccttcca tgttttttaa tacattgtca tttgctatac tttcatcttt taaagtaaaa
    19801 cgtaatcttg taatacagtg tactaaccca ctaatatttt cttttccacc aacattgttt
    19861 actatctctt gtgctaattg tttgtatttt cccatttttc tcctccatat ttcgtttaaa
    19921 ttaatttttt gaaatttgaa ggtttggcca actcaatgtt accatccaac cataagtata
    19981 attttgttag tatcactcct ttcaatcatt cttaatatac tactgaaaaa ttatttcact
    20041 tacatatttg agtctttaac tactttagct aaatgtattg ttaaatatat aagctcatca
    20101 tctgttaata tgtagttata ttgtttttta atgaaatttt taattttaac tacacattca
    20161 aactctttat tatatttaga tttaataatg cttagtaatt ctttgtctgt ctcaccttca
    20221 tgtgcactat ctaaaagcaa tctttgcgca aaaaacttta aatgcattat aaatctataa
    20281 taaaacactg attcttcata aaattctatc ctaaaatgat acctaactat gctaagtact
    20341 tcttgtatta acttcgtaat atcagatgcc aaagtatgac cttcacttaa ctgagcattc
    20401 actatatgaa aggctataaa tccagcctca tcttctggta attttatacc tgttttgttt
    20461 tcaataatat ctagtgcttt cattccaatt tcaaactctt ctttataaaa tcttttaatt
    20521 tcccaaagga ttgcattttt tacatttatt ccttctttca ttctttgaat tgcagaatat
    20581 gtatggtcac ttaaggatat gtaaatactt tcatttagct tcttgtttaa ctttatttgt
    20641 gcgtattcta taatttcact tgataatttc atgtgttcta taggaatatt attgagtaat
    20701 tcttgtagtt tgtttgatat ttctttattt gatattctaa atactttatt tactttagtt
    20761 ttatctatta gattgccttt tgatttttga taaccaattc ctttacccat aacgataact
    20821 tcttcatcat tatcatctaa tgtgattaca acattattgt ttaatatctt attaattatc
    20881 atgattatcc tccttttaag cataaaaaaa gcttatccat aataatgggc atactccttc
    20941 ctaggatact gctattatta atgaataagc ttagcttgta cttaaacaat tactacctaa
    21001 ttccaattaa tatgataaaa catcagacat attttgtcaa ctttttattc catatatttc
    21061 atcaatcttt tttgttaatt ataataatta ctatcattaa cattaactta tttctttaca
    21121 tcaatataaa tataaaacaa attatactca atactaataa aaatactatt aaagtaaata
    21181 gttgatatta tactctatcc actacattat ctgtactttc cttaacattt ttttcaactc
    21241 caaaaacata tgtcataata aatggagcaa ccaatgctat aaatataact ataagtccat
    21301 ttactatatt tgaaatcccc ttttcccctg ataatccaag tactgccatt atactatttt
    21361 gtggaacata taaagtcaca tgagtaattc ctgcaaataa ccctgcgatt cctgctccta
    21421 tagatgctaa catagcaggt tttttaagtt tcaatgtaac tccataaaga gctggttcag
    21481 ttatacccat gatagctgat atccatgcag ctgatgctaa ctgtttcatc ttactatctt
    21541 tgcttcttaa tgctacagct aatgtcgcgc caccttgcgc taaattagca caaacttgag
    21601 atattaatat tagcgattct tgtcctgtag ttgcaaatgc tgatatgaat ataggagtca
    21661 atgcatgatg catccctgtc ataacgataa atggcattgc agctgctaat aaacaaacca
    21721 ttataaatcc taaccttcct tgtagtaaat ttattatact agctaatccc tctcctaaga
    21781 aatttcctat tggtccaatt actattaatg ctattggcgc acttattaat aatactaaag
    21841 ttggatttaa aattgttttt aacatgctag gtgttaattt atcaatgaat ttttctatat
    21901 atgacatcat ccatactgtt aaaagtgcag ggataacaga tgctgcatat ataacacctt
    21961 gaattggtac tcctataaat gatatagggt ccccacttga tacccatgca gggaaatttg
    22021 gatgtaaaaa cacacctact actataactg ctattgatgt attaacatta aattttttag
    22081 ctgcatttat tgcaagtatt ataggcataa agtaaaatgc tacatctcct attatatcta
    22141 atactctata tgtacttgaa tttgatgata gccaattaaa agtggtacaa agagataata
    22201 taacttttat catcccagat gctgtaaggg ctggtatcat aggtgtcata caaccagtta
    22261 ttgtatctat tatattattt aaaatactat tttttttatt tttatttttt atatcgcttt
    22321 taatatctcc tgtggtattt atctccttat ttattattgc attaaataca acacctacct
    22381 cattacctat tatgacctga tattgagttg aagttgagtt tactccaact accttatcta
    22441 tagattttaa tttatctgca ttgactttct tttcatcttt taagatgaac cttaatcgtg
    22501 tcatacaatg agttaagtca attatattat cctttcctcc aagtccatca attatcaatt
    22561 gagctacatc ttcatattta ttcttcatac cttatcctct cttttaaaat aatttttaat
    22621 ataattttaa tccaaattaa tctttttact ccaagcttgt aatagtgtat tgtaatttaa
    22681 taagctagta tcttctacaa tatggtcaca ttttcctagt acttcttttt tccccacacc
    22741 aattgatgtc atattggcag catttatagc tttaatacct gaataagaat cttctattcc
    22801 tacacaatca tattgtttta aatttagaat cttgctccct tcaataaaaa tatcaggata
    22861 tggttttccc ctttttaata aagatggatt tactatacaa tcaaattcat cttttattcc
    22921 caaagattct aatatcctag gtgcattctt actagcagat gctattccca atttcacatt
    22981 atttttttta agttctttta taagctcatt tatccctggt aatatatctt tatatgttaa
    23041 tgagtttatt aactttatat aaatgttatt tttttcttca cacaccttta acttttcctc
    23101 tttagtataa ctatctactt tattagcaga taacaatatt ttatctaatg attcttccct
    23161 gcttatacct tttaattttt cattaaaagt ttcatctatt tctatatcta gcgattttgc
    23221 tatctcatgc caagcttcaa aatgatactt agcagtatct gttataactc catcaagatc
    23281 aaataagact gctttcatat taattactcc ctttaaaaca attattaact tctattatgt
    23341 tctttgataa ttttatttct ttattatcat gtaatatggt tatatttcta cctttgatta
    23401 acttataata agatttatat tcttctatat ttacagaaac taaattatct ctaaataata
    23461 tattaaattt aatatttttc cagtgtttag gtatccttgg ttcaaaactt aatttatcat
    23521 tttttatttt cattcctgca aatccatata ctaaactcat ccatgttcca cccatattag
    23581 cagcatgtat tccatcactt gtattttttt gtaggttatt aatatccatt aatgctgtgt
    23641 ccataaaata gttataagct ttttcatgat ttccaatttc acttgccatc attccaaata
    23701 ttgaccttga caaagatgaa tcatgagtag ttattttttc atagtattca aaatctctct
    23761 tcttttgttc taagctaaac tcatcaggga aaagcaacgc tgaaagtaca gtatctgctt
    23821 gtttatttac ttgataccta tatattgtta atggatgata atggagaagt aatggatatt
    23881 tttcttttgg tgtattttta aaatcccata ctgatttttc taaaaatgaa tcgtcttgtt
    23941 tgcttatttt cttctcttca tcatatggta aatacatatt ttgagtggca tttatccagt
    24001 tttttacttc atattcttgt aatttaactt ttctaaatac ttcatttgaa aataaattat
    24061 gcttaaattt atctaccatc tgtactgcat atattaaatt atgtttagcc attaaattag
    24121 tgtagtaatt attgtttact attgctgtat attcatctgg tcctgttact ccatttatac
    24181 agaatttatt attttttgat tctatataat ctccaaattc aacccaaaat cttgctgttt
    24241 ctataagaat ttctaaacca ttttgcatca taaattcttc atcattagtt gcttcaaaat
    24301 acgttctaat accataagct atatctgcat ttatatgaaa ctgtgctgtt cctgctggat
    24361 aatatgcact gcattcatta ccatttatag ttctccatga atataagcaa cctttattta
    24421 ttccaagttc cctagctctt tctcttgatg catctaagat tgagtatcta taatctaata
    24481 aagctctagc aatggatggt tgagtatata taaaaaatgg taatatatac atttctgtat
    24541 cccagaaata atgcccctca tagccatcac ctgtaagacc tttggctgat atattagtct
    24601 ttccatctct tcctgctgat tgataaatat gaaataaatt ggtttttatc cccagttgaa
    24661 gagttttatc gccttcaatc tctacatcag aggttgtcca aaactcattc attttctttc
    24721 tctgaatatt tttgacttta tcaaatccaa cattacatgc ttctcttaaa accaattcta
    24781 atgattttaa atgtgcttct gattctaaac attttaacga ctccagacat tctaatgatt
    24841 ttaaacgttc ttcatttaga tttaaatcag aatatactct actatagcct acaacaattt
    24901 ctaaatttaa cctttcatct tttttggcat agacttctat ttctcgctca atacattcat
    24961 ctgaaacatt atgggtctca tttgtaatat ttccacaagt aatatatgaa tcaaacccac
    25021 accataaccc taatctactt ctttctgtaa ctaaattcat aaaatactta ttgttatatt
    25081 tttttacatt aattttaaat tgcttcttat ttatagatgc cactcttggg tcatctactt
    25141 tttcacaatt aagcttcttc atactatttt ccattttatt tttaaatttt atttttccat
    25201 taaaattcaa tggtgttatt gtatatgaaa tcatcattaa ttcttctata tcttgtgata
    25261 ctaatctttc tatagatact ttaacttttc taccacgaga gttttcccat ataaatgaac
    25321 gtttaagaat cccttcattt aaatctaata tcctagtatg ttcacttgta ataccttttg
    25381 ataaatcaaa ataatctcct tcaatagaga aatttactat atgagcatta ggtaacttac
    25441 agatagtctg attttgtttt gcatagccat aagcgctttc tccatatgta attggagatt
    25501 cttcataaaa tccatttaca tatgtaccca tattatctac atactcatta ttaaaatcag
    25561 actcttcaat attccctcta actcctaaat gtccattagc tagtgtaaac aacgtttcat
    25621 tggattttat tgtatcctta ctaaaccctt gctctataag ttggtcctta gtagtaaata
    25681 ttaaactcat aataacactc ctttcttaaa atgaattatg catgagtttc catatcatta
    25741 aataaaattt ctccatctgt ttctattact tttttatacc aaaaagcact ttgtttttta
    25801 gttcttttaa atgaacctct tccatagtca tcagaatcaa catgaattat gccatatcgt
    25861 tttgtcatct ttccttcact tacactggtt acatctatac atccccacat aagatatcct
    25921 ataagctcta ttccatcttc atttactcca agaatcatat ttttaatatg ttctttcatg
    25981 taatctatac gataatcatc aattattttt ccatcttcat ttacaatatc ttgagtaccc
    26041 agtccatttt ctactataaa taaaggtaac tgataccttt catataactg attcatagtt
    26101 attcttaacc ctacagggtc gttttgccat acactatttg attttggaag atatggattt
    26161 tttatggttt ctggtccgtt tgcctttgat ttttctaaac cttcagctct agctgaacta
    26221 caagcactag aataatagct aaaagataca aaatctacag tattatcctt aagtgttgat
    26281 aaatcttcct cttttattat taattttatt tgatttgatt ttaaataata attcataaag
    26341 cttggataca cacccctaac ttgaacatca gaaaaaaaca aattatgcct ttcatgctct
    26401 aaagttgcaa atacatcttc tggtttacat gtgtatggat atgtttttcc agcagctaac
    26461 atacacccaa acttaaattc tttatttata taccttccta tttctatagt ttttgcatta
    26521 gctaataatt gatggtgtgc tgcttgatat tttttctgaa gttgattttc atcagctata
    26581 aaagttaacc cagcacctat atatggaata tgcattatca tattcatttc gttaaaagga
    26641 atccagtatt tgacatcatc tttatatctt ttcatcaaaa tttcagcata ttttacatat
    26701 aaattaacca ctcttctatc agcccatcca gaatattttt caactagaga ataaggtata
    26761 tcaaagtggc ttatcgttat taatggctct atattatatt tttttaattc ttttattata
    26821 ttatcataaa gttgtaatcc cttttcattt ggttcacttt cttctcctgt tgggaatatt
    26881 cttgaccaag atatagaaaa tctaaaacat ttaaaaccta tttctgctaa cattgctata
    26941 tcttctttgt aattttcaca aaatttaatc cctatatgac ttggatagta ttgattattt
    27001 tcatttaaaa tattatcctc atctaaacat acatctttta atctcttatc atttaatggg
    27061 agtttatcaa actgagataa accttttcct ccatctaaat aaaatccttc actttgattg
    27121 gctgaaattg cccctcccca taaaaaatct ttccctaatt tcatgcatct acctctttca
    27181 cagtataaag ttttataatt tctatctcat atttagttat aagatttttt tcatactata
    27241 ccttttaaat aattcaattt aaaaatccaa ttatattttt tactcactat aattaatatt
    27301 gtctattttc aatactttca tattaaatat caatattaga tatctatgta tataattata
    27361 tatatagcaa atacattttt atatcctcat tattgttatc ttattctcat atattgctgt
    27421 atatttatat atataaaagg atttatttcg aaatagaggt tgtattgaga actaaaattt
    27481 agactttata aatgaatgga attttcattg ttgaaatact gaaaatatat ttgatttcat
    27541 ataaaagaaa gtgcctcatg aactaagttc attttgagac actccctttt ttctccaagc
    27601 tattatatct ttttttaaat tctaatggag agactccaat tttttcttta aatactctgt
    27661 aaaaagtttt tgtacttcca aaaccaactt cttcaaccaa atctacaatg gatatattgt
    27721 tattaagtaa tatccacttt gctttttcta atctatattc atttaaaaaa gttataaatg
    27781 tttttcctgt attttttttg aaaaatttag taaaataata tacactaaat cctacttcat
    27841 aagacacatc ttctaatttt ataggttgtt tataattttt ttctacatat ttgaatatat
    27901 tgttcaatct ctctaatatc tctttagagt ttatttcata ttctactgta ttttcttcaa
    27961 tttctctagg aatctgtcta tataatatag gaattaaatt atacatttta ccttttattg
    28021 cataactata tccttctatt ttgtataggt cttcattgta gatatctatc aatatatcga
    28081 aaactgactt tcttattttt gctgaccaat ctctactaca tttttttata ctactgaata
    28141 aattaattaa gctttctttt tcctcattta ataaaattaa atcattaaaa aaagatatat
    28201 caaattgaaa tactaatctt tcacttcccg gtgatggcag tatataatga atatctcctc
    28261 cttttattat cactatctcg ccttttttta attgcatagg cttgtcattt ataaatagat
    28321 ttactacacc ttctgttata agtaatattt ctatttcctt atgccagtgt ggtgttgtta
    28381 atacttctcc ttcatttgat agactacgaa aaggaaattc atgtattaat ttaggcattt
    28441 ctaaatatat tgccatatat attctctcct ctctagtgtc taaaaattaa tctatactat
    28501 ttaaaagaat acacatttta ttttaaaaac ctcaaattta taatttatac aatacctaca
    28561 cttcataaaa tatatctaat atttcttttt ttgtatgtgc atttaaaagt ctttcaccta
    28621 tatttttacc acctaaaatg taagctattt tagatattaa tatcatatct tcattgcctc
    28681 cagattgtgg catagcaaac ataattatta cctttacagg taagccatct atacttggcc
    28741 aatctacacc ttttttagat ttacctatta ctatggatgc cttctttaca tattttgttt
    28801 ttccatgtgg catggccatt tgataaccta tactagtaga gcctattttt tctctatgtt
    28861 ttaatgcctt tataaactta ctttttaaag ttatacttcc tgtatcgtat aatttatcta
    28921 ctaattcctg tattatgcct tcttttgtag ttgatttcaa atctaaaata atgctttctt
    28981 ctgttaatat ttctctaatt tccatttgtt ttctccaata gattattctt aaataatatt
    29041 ttaatatcct ctttgctctt gcaatctatt atatagttta tatagttctt ttgcctatat
    29101 aaatttgata tttccattaa aatattcttt aaaaaacttt gattactaat ttctaaataa
    29161 atatcaatcg ttctagtaac attatttata ttcattatcc ctttatttaa tttactttgc
    29221 ttatgtatat atatatttaa atcttttcct aatgaaatag attcatattt actccctaaa
    29281 ttcacaccta ttaaactttc catctgagct acatcaaaat taaaatatat attatcttct
    29341 aaatacattc ctaataagtt tgtatctaag ttcatacttt ttttaggttt agaaagtaaa
    29401 gcatttttta tattattaat atcactttcc attaatagta ctgatacaac tatatgtaat
    29461 ttatctttca aatctaagtt tacagtagat attacaaagt ctacatcatc taaatttcgc
    29521 ttactcaact gatgcatagg aactatatca actatagtcc attcaggaaa ttctcttttt
    29581 aatctggctg caaggagctg agatgttcca tatcctgtat gacaaactac tattactctt
    29641 ttactactca tatttctttc tattgccgct tgaaaatatg tggcaagata cgaaacttca
    29701 tctatactaa tattcttcag attatcatac tctgtcataa tatttatagt catcatacat
    29761 aatcccagca catctgaaaa tcgctcttgt atctctccta acaacgggtt ttttatttgg
    29821 atatcatatc ttaatctgtt taacataggc cttacatgag atgctagact tttctgtagt
    29881 aagtcatcat ttttgagatt tatgtttaaa aatcgtgaca tattctctat cattatagat
    29941 gaaacttttt cactttcact taatctatct gattcatcat tgtaactact attattttcg
    30001 ctactaaatc cagatgatac caaaaaaata tatatgtaca taatttcatc tttcgttatt
    30061 tttactctgt atctattttc tattttatct ataagtgtag tgacattttt atatactaac
    30121 tcgtccaaat tatctacaga aatttctctt tcttcctgag attctatttg attaccttct
    30181 tcgattctct ttaaacatat aagtatatgg gtaattaaat ttatatagta gggctgattt
    30241 atagtatatc ctaaattgta ttctaattct gtaattatag tttctacaaa tataatagaa
    30301 tcaatatcaa ataaatttat caatgcacta aatgtagcag aatctaatct tgttaactca
    30361 gatatatcat gttgttcata attttcatct aataaatcat ccatcatttt ggcaatcgat
    30421 ttccttatat ccacttcttt acctataatc ttagttcctt ctaaagtttt attaagtttt
    30481 aaattgtatt cttttatcca tgcctctata tatttaaaat catttactat agaagtttta
    30541 cttacataat atttatctgc taactgattt attgaagtta tattatttga atttagtaat
    30601 aaatacttta aaatctccat tcttctctgc tctactgata aagatttatt ttccttcgat
    30661 tgtatattta aactattaat taagtctatg ttcttctttg ctaaatcttc tattaatatt
    30721 ccatagcctc tttttatatc aatttttata ttaaaagttt ctaaatattt ctcaatcatc
    30781 ttcaaatctt tttgtaaagt tttatcagat acatttaatt tatctttaaa gtattttata
    30841 ggtctatatt tattttcgtt tagtatcaat ttaagaaatt caatttgcct cgacacaagc
    30901 atacttagcc ctccctacct atgtttatat atttctatgt ttatattaag tactatttta
    30961 acatatttac taactatgta aaattactaa gatacttctg tatcataaaa gaatttatga
    31021 tacaccttac taaattgata taatagataa tataatttca aaataaaaat taaggatatt
    31081 tagaagtagt taactgattc taaatatcct tattattatt ctatatactt gttgttattc
    31141 cattaattat ttttactatt atttataatt taagcaccaa gtaattcaac ttcactattt
    31201 tcactcctta tagtaggctt tgttacccct ataatcaatg ctgttattac tgtaccaatc
    31261 aatatacaag ctatatatgc aactggtgca ctacatgcaa atggtattac aaatattccc
    31321 ccatgtggtg ccattaaagt tatatctaaa ctcatacata ttgccccacc tatacaagaa
    31381 ccaattatat ttgctggaat cattcttaat gggtctgcag ctgcaaacgg tattgcgcct
    31441 tctgttatga aagatgcccc cattatagca gttggtagag atgactttct ttcttcttta
    31501 gtaaacttat ttttgaatat taacatagct aatgatatcg ctaatggagg taccattcct
    31561 cctaccatta atgctgctga tggttcaaat atattacttg aaaacatagc caatccaaaa
    31621 gctgaagctg ttttattaat aggtcctccc atatctgatg ccatcattcc tgctaatatt
    31681 gcacctaata atactttatt agttccagac atactttgta accaatattg taaagcttca
    31741 tttaaccatg atgctggcgt attaactaca aagaacatta tagctccact cgtaaatgca
    31801 atcaaaagtg gacatatcaa tacagcattt actcctttta atacatctgg taaattttct
    31861 gttatcttca ttactcctaa agctatatat cctgttgcaa aaccagctac cataccacct
    31921 ataaaccctg aacctccaat aactgccata aaaccagcta tcatagcagg agcaaatcct
    31981 tgtcttcctg ctattgattt agctataaaa ccagcaagta tcggtataaa taacttaaat
    32041 gctgaatcgc ctccaagttg tccaaaaaat ccagctatag ggttgtatgt ttcatgagtt
    32101 gggtctgatg caaatatacc aaacataaat gctatggcta taccaattcc acctatcact
    32161 acaaatggaa tcatttcaga tacaccactc attaagtgct tataaacacc ctttttctct
    32221 gacccttgaa gcatttcttt tgaatcttct aaagtctttc cacctggttt aatttcttta
    32281 gccattccat tttcagcgtt tataattaat tttttagcta atgaaacatt cttagcatct
    32341 actactttct tttgtagcac tttctttcct ataaatctac tcagttgaac ttctttatca
    32401 caagctatta ttacgtattc agctcttttt atatcttctt tagtcaactt attttctaca
    32461 cctatagctc catgagtttc tattttaact tcatatccaa gttcttgacc tgcttcctct
    32521 aatgcttttt ttgccataaa agtatgagct atacctgttg gacatcctgt tatcccaact
    32581 ataagacctt tattttgatt attactaaaa tctcctgatt ctgcagcttt attattaaat
    32641 atacttaata tttcgtatgg agtttctgca cttagtagat gcttaagaaa attttcttcc
    32701 attagatatg tagctagttt agatataatc ttaagatgtt catctgaacc tgattctgga
    32761 actgctatca taaaaaatga cctaagtttt tgaccatcat tatctgtaat ctctctatct
    32821 gttcttaaat acatcattac aggttgattt actacatttg acttagcatg aggtatagct
    32881 attaaattac ccatattagt tgatgattct ttttcacgtt tattaatatc agctataaac
    32941 ttttctttat tatttatata atttccatgg tacaaatgat ttgccatata attaattaca
    33001 tctttttttg tttgtaattg tttatctagt acaatacaat cgcttcgtag tattttacct
    33061 atcataaatt ctcccctcct tttaatttgt tacctcataa ttaattttta tttttataaa
    33121 agcactttaa cttatctata gcattgaata gattcatttt aaactctata acttctcttg
    33181 cagcttttat acatggtgga tatatagtat ctggctcata taagcttggg ttttgttcta
    33241 atagttctct gcacttctta taatatgccg acttaacatc actcgaaata tttattttac
    33301 aaactcctag tttaactgct tgtgctattt cttcatctgg atttgatgaa ccaccatgaa
    33361 gaactaaagg tatatctact acttctctta tttcttttaa taaatctagt ttaagctctg
    33421 gtttaaaacc ttttggatat ataccatgtg ctgtaccaat tgcaatagct agtgtgtcta
    33481 ctcctgtttt ttcaacaaaa tccttagcta attttgaatc tgtatatatt atttcatcag
    33541 taccaccttc acttgatgtt cctgttgttc ctattgttcc aagttctcct tctacggaca
    33601 catttaatcc ttttgctatt tctactattt ccctggatat agctacattc tcttcatatg
    33661 gtaaatgtga gccatcaatc attactgatg taaatccaca tcttattgcc tttaatattt
    33721 gttctttatt ttccccatga tctaagtgta ttaccatagg tactttactc ttatttgctt
    33781 cttctatgca actagctaca aagctatctc ctagaaaact tagctcattt gggtgtattg
    33841 caagtattac tggtgcattt ttttcgttag cactctgtac tactgcttta agtatttgcc
    33901 cacttcctat attaaatgct ggtacagcaa attgattttc ttgtgccact tttagcattt
    33961 ccttcatatt tattaacata ttgtacctcc cgatatttta aattttaggt tgaatttgct
    34021 tcttgttaat tttgttgttg tttatattta aataatatca gggtaatgaa ggtcagaaaa
    34081 taacaatcgt tttccttagt gaaaaggaac ttttattata cactctaagt tccttttcac
    34141 taaataattt ttagaagtct gcatcatagt taattatatt tctcatgtac atcttctctt
    34201 tttaagtgtt ttatgcttta gccttcctag attttataat aaacaattaa gatacattaa
    34261 ctataaattt aaatatactc atgcaatagt taatgctata ttagagtcaa aaaaattaaa
    34321 aagatattag ctatttggcc aatacctttt cttggattcc aatagtatat acgatttata
    34381 gaaatcatac tcacgattat ctaatgtttt acttccataa actttacata aaacaccaaa
    34441 gtgacaccct ccaaattcat ctctagctaa taaagtgatt tggttttcta agcaccaatt
    34501 tataaattag cctttgttat cttatgtaaa tctttttcta tctaatttaa aaattattcc
    34561 taatgctata tagcatatac caattactaa tacaccataa gccatatata ctcctatctt
    34621 aaattcttgc atattaatta caatagaaac tgaaataggt ttattacaaa caccataaag
    34681 caaggctgac attgtatatt atccaattgt atgaatgaat actaatatag ctccttttaa
    34741 ttaaatgatg ctatataaat catatttatc ccaattttta atctaaccta tttatatgta
    34801 tattcctttt taataaaaaa agatattgac taaatagtca atatctttta gttgatttaa
    34861 atac
//
LOCUS       DAEMHW010000018        34579 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEMHW010000018 DAEMHW010000000
VERSION     DAEMHW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34579
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.018"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            528..1550
                     /locus_tag="KPK67_000763"
     CDS             528..1550
                     /locus_tag="KPK67_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG2457764.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            1846..2766
                     /gene="pyrB"
                     /locus_tag="KPK67_000764"
     CDS             1846..2766
                     /gene="pyrB"
                     /locus_tag="KPK67_000764"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBG2457765.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            2805..3497
                     /locus_tag="KPK67_000765"
     CDS             2805..3497
                     /locus_tag="KPK67_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBG2457766.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            3490..4392
                     /locus_tag="KPK67_000766"
     CDS             3490..4392
                     /locus_tag="KPK67_000766"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG2457767.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            4477..5061
                     /locus_tag="KPK67_000767"
     CDS             4477..5061
                     /locus_tag="KPK67_000767"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBG2457768.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            5277..6203
                     /locus_tag="KPK67_000768"
     CDS             5277..6203
                     /locus_tag="KPK67_000768"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBG2457769.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQVKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(6255..7145)
                     /locus_tag="KPK67_000769"
     CDS             complement(6255..7145)
                     /locus_tag="KPK67_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2457770.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSV"
     gene            7333..8364
                     /locus_tag="KPK67_000770"
     CDS             7333..8364
                     /locus_tag="KPK67_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBG2457771.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            8658..9533
                     /locus_tag="KPK67_000771"
     CDS             8658..9533
                     /locus_tag="KPK67_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HBG2457772.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRIYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            9551..11041
                     /gene="cls"
                     /locus_tag="KPK67_000772"
     CDS             9551..11041
                     /gene="cls"
                     /locus_tag="KPK67_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG2457773.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            11234..11518
                     /locus_tag="KPK67_000773"
     CDS             11234..11518
                     /locus_tag="KPK67_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBG2457774.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            11551..13179
                     /gene="groL"
                     /locus_tag="KPK67_000774"
     CDS             11551..13179
                     /gene="groL"
                     /locus_tag="KPK67_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBG2457775.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            13441..14181
                     /gene="larB"
                     /locus_tag="KPK67_000775"
     CDS             13441..14181
                     /gene="larB"
                     /locus_tag="KPK67_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBG2457776.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            14265..14999
                     /gene="larC"
                     /locus_tag="KPK67_000776"
     CDS             14265..14999
                     /gene="larC"
                     /locus_tag="KPK67_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBG2457777.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            15029..15448
                     /locus_tag="KPK67_000777"
     CDS             15029..15448
                     /locus_tag="KPK67_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBG2457778.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      15508..15609
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15830..17365
                     /gene="guaA"
                     /locus_tag="KPK67_000778"
     CDS             15830..17365
                     /gene="guaA"
                     /locus_tag="KPK67_000778"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBG2457779.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            17904..19334
                     /locus_tag="KPK67_000779"
     CDS             17904..19334
                     /locus_tag="KPK67_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2457780.1"
                     /translation="MILNQIFIESGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVSGSSMDSYFSARYKDID
                     NNTERYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(19482..20357)
                     /locus_tag="KPK67_000780"
     CDS             complement(19482..20357)
                     /locus_tag="KPK67_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2457781.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETALFDRLPRGVCLTRCGISLLEEARSILTACRNLDKRISRLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACSPTFPLSKQALSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFALIKAAEAGLGITILPENLLLDSLFHKKLRLIKLDGIDMENKMLAILHKDKNI
                     TQPLKIILDNISNVL"
     gene            20477..21040
                     /locus_tag="KPK67_000781"
     CDS             20477..21040
                     /locus_tag="KPK67_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG2457782.1"
                     /translation="MNQSRKTYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCIAAIIPPLVILGLVSAF
                     YTTFISNAIVTAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSAMIPIAFFANFVM
                     SINVIFILSVCCFVCVMQAFYKKRENK"
     gene            21037..21603
                     /locus_tag="KPK67_000782"
     CDS             21037..21603
                     /locus_tag="KPK67_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG2457783.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     TLLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(21732..21968)
                     /locus_tag="KPK67_000783"
     CDS             complement(21732..21968)
                     /locus_tag="KPK67_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457784.1"
                     /translation="MLECFIAKDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(22278..25706)
                     /gene="uvrA"
                     /locus_tag="KPK67_000784"
     CDS             complement(22278..25706)
                     /gene="uvrA"
                     /locus_tag="KPK67_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG2457785.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIRKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKTGSQNKTIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VLHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            26025..28259
                     /locus_tag="KPK67_000785"
     CDS             26025..28259
                     /locus_tag="KPK67_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2457786.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLKGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSDKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            28515..30545
                     /locus_tag="KPK67_000786"
     CDS             28515..30545
                     /locus_tag="KPK67_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2457787.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKDWESIDFIITTSK
                     IAIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            30542..30994
                     /locus_tag="KPK67_000787"
     CDS             30542..30994
                     /locus_tag="KPK67_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2457788.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            30996..32051
                     /locus_tag="KPK67_000788"
     CDS             30996..32051
                     /locus_tag="KPK67_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBG2457789.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLSSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            32118..32426
                     /locus_tag="KPK67_000789"
     CDS             32118..32426
                     /locus_tag="KPK67_000789"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBG2457790.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            32530..33795
                     /locus_tag="KPK67_000790"
     CDS             32530..33795
                     /locus_tag="KPK67_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBG2457791.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            33883..>34579
                     /locus_tag="KPK67_000791"
     CDS             33883..>34579
                     /locus_tag="KPK67_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG2457792.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGVITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKI"
BASE COUNT        13203 a         3838 c         6123 g        11415 t
ORIGIN      
        1 gatatattat ctaaaaaaat taagtatgaa aagattactg ctaggacaat cttttcatac
       61 ttaattttta atatttatat tctccaaaat tttcatcttt ttaacttaga atttgagaat
      121 cactattagg aaataggctt tgatttatgt agggttaaaa atttcacaga atatctaact
      181 ttatactaaa tccatcaaaa aatatacgcc atataaaagc gcataaaaaa acacataaaa
      241 caatatttat gcgcacttta acctcgaatg gtacaaaatt ctttactttt acagacccat
      301 attatataat taagtaaata atgtatattt aggtgacata ttggataagc taatttattt
      361 attttattaa tataatgata ctataaaagt tatataaagt ggtttacaaa aaagttacag
      421 atagagcttt tattaatgta ttgtgtatta gtattttatc cttaggtaca cttcacctac
      481 aaaatagaat cataagataa ttatgggaaa attttaggag gtttatcgtg aagaaaaaga
      541 tattaatacc agtatttgca tcagttatgg cattatcagt aagttcaatt gtaaatgcag
      601 atgaagtaaa tgattcatct caaaataagg atgataaaac aaatactgaa ttaaatttag
      661 gtgaatataa agaagtaaaa tataaagttg caaaaataaa agatggagta gctataaaga
      721 taagagaaga aggtcaagtt caaaacatag cttattctgg agatgaattt acggttttag
      781 gaactcaagg agaatgggtt aaggttaaag ttgaagatgg cgaaggttgg ttagctacta
      841 gatatgtaga tatatctgaa ggtgtaggat atactaacgc tgataaagta aacttaagaa
      901 aagataagag tgaaagttca gaagtaattg aagagctgga aaaaggaagt tctttattgg
      961 ttttggaaga taatggagat tggttaaaag ttaaagatgg ggaaacagaa ggatatgtaa
     1021 aatcatctta tatatctgat aaagctcctg taatagaaga gccacaagta aacccaagta
     1081 ttgaccaaaa tgtagattct acaaataata atcaacaaaa caatgcaaat actaatcaac
     1141 aaaataataa ttcaaatgtg ccaacagcta actctaatgc agttcaagct gtacttaact
     1201 tggcatattc taagcaaggt tgtccatatg tttggggagc agaaggacca aacacttttg
     1261 actgttctgg atttacacaa tatgtgtata gaaatgcagt aggaaagaat ataccaagaa
     1321 catctaaagc acaatctaaa tatggacaaa ctgtaagtaa agcaaattta caaccaggag
     1381 acttagtatt cttcacaact aatggttctg gttctgttag tcatgttgga atatatgtag
     1441 gtgggggtaa tatgatacat tcaccatcaa caggaaaaac agttagtgta actagtataa
     1501 attcatcata ctatacagca agatttgtta cagcaaaaag aatattataa tcggtttgta
     1561 atatgacaaa ttgataaata cataaatata tataaacgct ctatattata tttaatttaa
     1621 tcatggaaac aaaaaatgat tatattttaa atgtactata gagcgttttt gtatcacttt
     1681 aaatctaatc aagattcaag ttaatatgac tgttaaatag gagccttttc atatgatgat
     1741 tatttgtgca atcccttgac aggatagggc aaaggtggta agctactaac agtacaaaaa
     1801 tacttttaat tttagttcag agaggctaaa aaaggaggac gaaagatgtt aaaatcaaga
     1861 aatttaatcc aaccagaaga cttttcaata gaagaaatag atgaaatatt agaattagct
     1921 caaaaaataa ttgacaatcc gtcaaaatat tctcgtatat gtgaaggaaa actattagct
     1981 acattatttt atgaaccatc aacaagaaca agattaagct ttgaatctgc tatgaacaga
     2041 ttaggaggac gtgtagtagg cttttcagag ccaaattcat cttctgcatc aaaaggtgaa
     2101 actctaggag atacaatgag gatagtatct ggttatgttg atataatagc tatgagacat
     2161 cctcaatcag gtgcagcttc agaagcagct agatatacgg aagtgccatt tataaatgca
     2221 ggagacggca aaaaccagca tccaactcaa acacttacag acttacttac tataaaatca
     2281 ttaaaaggaa ctttagaaag ccatacaata ggattatgtg gtgatttaaa atatggaaga
     2341 actgtgcatt ctttggttaa ggctatggca agatataaaa atacaaagtt tgtatttata
     2401 gcgcctgaag agttaaaaat gccagattat ataaaagaag ctataaaggg acatgcatat
     2461 tatgaaacta ataatttgga tgatgttata ggaagcttgg atgttttata tatgactaga
     2521 gttcaacaag aaagatttga agataaatca gagtatgaaa gacttaaaaa ctattatatt
     2581 ttaaataaag ctaaattaga aaaggcatct aaagatatgt tagtaatgca tccattacca
     2641 agagtaaatg aaatagacat agatgtagat agtgatgata gagctgtata ttttaaacaa
     2701 gctaaatatg gaatgtatgt tagaatggct ttaataataa aacttttagg tataaatgaa
     2761 gactaatagt tatcatataa tagtaaaaca aaggagattt tgggatgtat aaaattctgg
     2821 aaaatatata tataggcgaa gatatgtata gaatgaaagt aaaaggtaat tttgaaggta
     2881 aaatgggaca attctatatg ttaagagcat gggatactta tccagtactt tcaagaccta
     2941 taagtataca tgatatagat gaagaaggga taacttttct ttataaagta gtaggagaag
     3001 gaactcaaat tttgagtaat ctaaaggtaa atgataatat aaaattagaa ggcccttatg
     3061 gtaatggata tgctaaggtt gatggaaagg tagctctagt aggaggagga atcggtgtag
     3121 ctccactata tttggttgct aaaaatataa aaaattgtga tgcatatctt gggtttagag
     3181 aagatgttat attagaagat gaatataaac aagtatgtaa taaagtctat acaacagtag
     3241 gaaatacttt tgtaacagat ataattgatg ttgagaaata tgattatata ttaacatgtg
     3301 gtccaactcc aatgatggaa aaattagtta agatggtaga aggtactaaa acgagaatta
     3361 tggtatctct tgaaaaccat atggcttgtg gagtaggtgc ctgtttagta tgtacgtgta
     3421 aaactaatgg aggaaataaa aagacttgta aagatggtcc agtgttctgg ggagaggacg
     3481 tgatttttaa tgggtaattt aagtgtgaaa tttggaagtg tagaatttaa aaatccagta
     3541 ataatggcgt ctggaacttt tggatttgga aaagaatata atgagattta tgatatacaa
     3601 aaacttggtg gtataagtag caagggtctt actttaaata aaaaacctgg taacaatggt
     3661 atgagagttc atgagacacc atccggtatg atgaatagtg ttgggcttga aaaccctgga
     3721 gttcaaggat ttatagacta tgaattacct ttttttagta aactagactt agtcagaata
     3781 gctaatgttg gaggtgggac attagaagac tacttattag gggttcaaat gttaaatgac
     3841 aagcctatag acattattga attaaatata tcttgcccaa atgtcaaagc tggtggaatg
     3901 gcttttggta taaaaaatga agtggcaaga gaagttgttc gtgaggtaag aaacataacg
     3961 aaactgcctt tagtaataaa attatcaccc aatgcagaag atattgtagg aatggcaaaa
     4021 gtttgtgaag aagaaggtgc tgatggagtt tcattagtta atacttttaa agcaatggca
     4081 attgatataa aaaatagaag accagtattt gaaaatgtat atgctgggtt atctggacca
     4141 gctataaaac ctatagcttt gagaatggta catgaagtgt gtaaaaatgt aaatatacct
     4201 gtaatgggaa tgggcggaat aactaaagcc acagatgcta tagagtttat aatggcagga
     4261 gctacttgta tccaagttgg aactgcaaat tttatgaatc ctagaatagg tattgaaata
     4321 atagatggaa ttaatgaatt tatggataga gaaggaataa aaagtttaga cgaaataaga
     4381 ggaattatat aaggtagaat aaatagtatt ttaaagataa aaaatataga ttaaataaac
     4441 aataatttta taacattaac atggaggaaa aataatatga gtaaagtaga tgtagtggat
     4501 atattaaaga aaagtgatgc attgttggag ggacattttt tattatcttc aggaaaacac
     4561 agtaatagat acgtacaatg tgcaaaagta ttaagattcc cagaatatgc tgctgaggta
     4621 ttaagtacag ttgttgaaca aataaaagac ttagatatag acttagtagt aggaccagct
     4681 atgggtggag taatagtttc ttatgagtta ggaagacaat taggaaaaga agctgtattt
     4741 actgagagaa aagacaatac aatggagtta agaagaggat ttgaagttaa aaaaggagca
     4801 aagataataa ttgctgaaga cgttgtaact actggtaaat caactatgga gacaaaaaga
     4861 gtactagaag ccttaggtgg agaagttgta ggtgttgcat gtatagcaga tagaactaat
     4921 catgatatag gtatgcctat atatagtgcc ataaaacttg atattcaagt ttatgaatct
     4981 gatgagtgtc ctttatgtaa ggaaggaaaa ttaccagttg ttaagcctgg aagtagagag
     5041 ttcaaggaat tagggatgta ataaaaactt aattatttat agtgttactt aaaaaatgta
     5101 aattttttag agtaaaaatt tgtaatataa catataactt aaaaataaaa agcagttagc
     5161 cttataaatg aaggttagct gttttttttt gttaaaaata tatttacaaa ataagagata
     5221 gtagcagtac aatataagta taaggtaaaa gtattatata ttagggaggg gtaagtatga
     5281 aaaatcaatt aaatgaaaat cataaaaagg ttgtattttt tggagtaggt gctgtaggag
     5341 ctacttttgc agaacaattt tttaactcta aatatgattt taaaattctt tgtgataatg
     5401 aaagaaagaa aagatattta gaagaaggat ttataataaa tggaaaaagg tatgattttg
     5461 attatgtaac taaagatgag tataaacaag aggctgattt tataattata ggtctaaaat
     5521 ataataattt aaaagaaaat ataaaagaat tagatggatt agttggaaaa aatacagtta
     5581 taatgtctct gctaaatgga gttgatagcg aagagataat aggagaaaga tttggaattg
     5641 aaaaaatggt atattcatat gttaccaata tagatgcaaa gaaaatcaat aataatatta
     5701 tacacactac taatgggata attgtatttg gtaacaaaga taatagtgaa gatagaaaaa
     5761 ctaatataat aaccgaagtc tttgatgatg taaatataga atatacttta tcaaaagata
     5821 ttcaacgaga tatgtggtgg aagtacatgg ttaatattgg tgtaaatcaa acttcagcta
     5881 tacttggtgc accttatgga gtttttcaga gttctgagca tttaagagaa ttagcaaaat
     5941 ctgcaatgag ggaagttgtt gctatagcac aagtaaaaga catatctctt acagaagatg
     6001 atgtggaaca ttcattacat agaatactag aacattcaaa agaaggaaga acatcaatgc
     6061 ttcaagatgt ggaagctcat agacttacag aagtagatat gttttctaag aatatctgca
     6121 aacttggaaa aaaatataac atacctactc ctataaatca gactttcttt tatatgataa
     6181 aagtaattga aagtagattt taaagggtct ttgtaagaaa tattgataat tattgatttt
     6241 aaattttatt aatgttatac actatttaag ttactaaata tttttaactc ttcaatgaaa
     6301 atagttgctg cttttgataa agttatatct tttggaaata tatatgagaa atgtctgttg
     6361 tagctgcttc caagctctat gacagacagc tcattgttta gaacaggtaa gcttgtcact
     6421 agattagaga ctattgtaat tcctaaatta tttctaacag attctttgac agcataatta
     6481 cttccaaaaa ccatcatact ttttggaata atttctttaa cacttaaaaa catatctaaa
     6541 taatctctgg ttccagaacc atcctccctg actatccatt tttggttttg aagtttatca
     6601 aaactaaaat cttttaataa atgactcttg tatggaagtg ctaaaaccat cttatcttca
     6661 aaaaaatatt cttgaataaa tgaaggggaa gagcaagtgc cttcaataag accaatgtct
     6721 aaaatataat ccttaacatg agaagatacg attgatgtat tttttataaa aacttctaca
     6781 tctatgtcag gatacttttt agaaaataat gctagaaaat ttggtaaaat atactctcca
     6841 atagttaaac ttgcaccaat ctttaagtga ccagttatag catctgaaac ttggcttaca
     6901 tcatgataag taatatttaa aagatttaat atttctttag ctcttttgta caagatttgt
     6961 ccactttctg ttataaaaat ggttttttgt ttaatagacc tgtttataag cacaacttta
     7021 aaataatttt ctagattttt tatgtgttta cttacacttg gttgtgatag atttaaatat
     7081 tccccagctt tagtaaagtt tttgtattca acaacagcta caaatgtttt taattcttca
     7141 aacaattttt tgtctccttt acctaatgaa attttacatt ttttatatac tttctacaga
     7201 taattatact atatataata accattaaaa acattaatca ttacgatatt atatatttat
     7261 tttacttatg gttattacaa ttatatacta ttgctatgaa gaaaactata ttaattataa
     7321 ggtaggtata ttatgttaaa gaaaaaaagc agtttgacat taaaaactat aagggaaata
     7381 ttaccaggac tgtttgtatc agtattagta ggttatatta gtatatttat ttctatttta
     7441 attcctaagg taggggcagc atcaatttct atatttttag gtatgtttgt aggaaaccta
     7501 tttttaaatc aaaaggtatt tcaaaaaggt tataaatttt cagagacaga tttgttatca
     7561 tattcaatag tactattagg agcaacactc agtgtatcta ccttaataga cttaaaagtt
     7621 tcaggaattt tatttataat attgcaaatg acaataacaa taatagctgc actttatata
     7681 ggtaaaaaac taggatttga agaaaacttt agatttctaa tggcaagtgg aaatgcagta
     7741 tgtggttctt ctgcaatagg agcaacagtt ccagtggtta atgcgacaga taaagaaaag
     7801 ggaattgcag taactattgt aaatgttact ggtatatttc ttatgttttt acttccaata
     7861 atatctcaat ttttatatag ccatgaactt gtaaaaactt cagcaatgat aggtggaact
     7921 ttgcaatcaa ttggtcaggt tgtagcaagt ggagcaattg ttggagaaaa tgtaaaagac
     7981 ttagctacta tttttaagat agtaagagta atatttctag ttgttgttgt attagtattt
     8041 ggacatatga aaaatcaatc taatagggaa attcttgaag aagaaaagga tgagataaaa
     8101 aagaaaaaag tgactatacc atggtatgta attggattct ttataacttg tgcacttttt
     8161 tcaatgaaca ttataccaca agaagtatct gtgttgtgca aagaaataag taacaaacta
     8221 gaaataatag ctttagcagc aattggatta aaggttaatg taaaagattt agtgaaacaa
     8281 gggaaagaag tttctttata tggtttattt gtaggtacag tacaagttgt atcagctgta
     8341 gttttaataa aaatatttat atagcataaa aatagtattt gtaatattaa tttgattaat
     8401 taaaataata tgatactaat aatttataat ataaataaaa aatgaatttc gataagttta
     8461 agtgggtata aacttataga aattcatttt ttatttaagg taaaaattta aagtaaaata
     8521 ttattttaca taataataac cttataaatt ttgaaattac ttatctttgt gttaaaataa
     8581 gtaaaatgca catattagag ctaagttttt aagtaaataa acaaaatgaa aatataaata
     8641 ttaaggagat ttggtatatg aatgtttatg aaaaaggtaa tggagttata ctagaaggag
     8701 taacagattt tgacccagta catatatttg agtgtggtca atgttttaga tggcacaaac
     8761 aagaagatgg ctcatacact ggagttgcaa aggggagaat attaaatgtc aaaaaagaga
     8821 atgataaaat atatttaaat aatactaatt taaaggagtt taacagcatt tggtataatt
     8881 actttgattt aggaactgat tatacagaaa taaagaataa gctaaaaaat atggatgaat
     8941 atttaaataa ggctactgaa tttggatggg gaataagaat attaagacaa gatggatggg
     9001 aaatgcttat atcatttata atttcctcta ataataggat tcctatgata caaagagcga
     9061 tagaaaattt atcaagaaag tttggtaagt atataggtga atatgaaggt aatgaatact
     9121 atgcattccc aacaccagaa gaactaaata aggcctcaca agaggaaata agagcatgtc
     9181 aaacaggttt tagagataag tacataaaaa gtactacaca ggcagttata gagaataatg
     9241 atgaggtatc tgaatataca aatttaagta cagaagattg tagaaaagag cttttaaaat
     9301 ttaatggagt aggaccaaaa gtatgtgatt gtattgcatt atttggtatg caaaaatatg
     9361 attcttttcc agttgatgtt tgggttaaga gagttatgca agaattttat atagatgaag
     9421 acatgagttt acctaaaatg agaatttatg gaatcgataa atttaaagaa atgtctggat
     9481 ttgctcaaca gtacttattt tactatgcaa gagaactcgg tataggaaaa taaattaagg
     9541 gggagaaatt atgggagtaa taggaacaat atttctgttc tatttaatta tatcatatct
     9601 agctggagcc ataatttctg tgattatatt acttgaaaat agagaccctg caaaaacaat
     9661 gtcttggcta ttgatgttta ttatatttcc aggtgtaggt ttaatgatat atgctatttc
     9721 aggtagaaat attagaaaaa gaaaattatt taagactcaa aaactagcta ataatataaa
     9781 agaaaagaaa ctatttgata cattagaaaa gataacagaa atagttgaat tagaaaaaga
     9841 atctataaaa caaaataagc ttttaagaga tgaagaggat ggtagttata gaaaaagggt
     9901 aataaatatg ttgttaaaga ctggtatgtt tccctttact aaaaataata aagtggatgt
     9961 atttgtagat ggaaatgaaa agtttaaaag acttatagaa gatataagag aagctaaaga
    10021 ccatattcat ttagagtact ttatcatcaa agatagtgaa ataggtaggg tgttaaaaga
    10081 agaacttata aaaaaggcta aagaaggcat taagataaga attttatatg atgatgtagg
    10141 atgctggaga ttttggttta atagaaaatt tttccgagaa atgagggaag taggtataga
    10201 aattgcagca tttttaccta caaaatttcc tataatagga gggaaattaa actatagaaa
    10261 tcacagaaaa attgttgtta ttgatggaat aattggatat acaggtggaa taaatatagg
    10321 agatgaatat ttaggcaaaa atgataagtt tggatattgg agagatactc atattaggat
    10381 taagggtatt tctgtataca tgcttcaaat gacatttctg attgattggt attatacgac
    10441 aaaagaagta ttagtgacta aaaattattt cccaagtgta gggaatgttg gtgaaagtat
    10501 gatacaagtt gttgcgagtg gtccagacag tgactgggaa gatattcatt atgcttattt
    10561 ttctgcaata tgtcaggcta gaaaaaatgt ttatatagaa actccatatt ttattccaga
    10621 tgaaagccta ctaaaagcta taaagagtgc tgctcttagt ggagttgatg tgagaataat
    10681 atttcctaaa attgcagacc acaagatagt aaatatagca tcttattcat attttgaaga
    10741 aatattaaga gcaggtggta aagtttattt atataataaa ggatttatac attctaaagt
    10801 cgtaataata gatgataaaa ttgcttctgc aggtacagct aatatggatt taagaagttt
    10861 tatgcttaac tttgaggtaa atgcatttat ctatgatgaa gaagttatta gggtaatgac
    10921 agatgatttc tttgaggatt taagtcactg tgaagaactt aatttggagg tatttaagaa
    10981 tagaaatatc atacaaaaaa tcaaagaatc tgtagcaaga ttattttcac caatattata
    11041 aaaaattatt aaaaaaggta ttgaaaatta tgtgtgattg gtatattata ataattgtta
    11101 gcactctatt agatagagtg ataataaata aaaagtatat atataaatct acatatataa
    11161 gtagtattaa tttaaaaata actaaaaaca taggcgaact agtctatact taatatttta
    11221 ggaggcgtga ataatgaaaa taagaccatt agctgacaga gtagtaatta aaaaagtaga
    11281 agcagaagaa aaaactgcaa gtggaatagt tttaccagga gcagctaaag agcaacctca
    11341 aatagctgaa gttgtagaag ttggaccagg tggaatagtt gaagggaaag aaataaaaat
    11401 ggaattgaca gtgggagata aggttatatt ccaaaaatat tctggaacag aagttaagat
    11461 agaaggacaa gaatacacaa tactaagaca gagtgatgta ttagctgtta ttgaataaat
    11521 atagaataaa tttattagga ggggtttaaa atggctaaag aaattaaatt ttcagaggaa
    11581 acaagaagag ctttagaagc tggtgtaaat aaattagcag atacagtaaa agtaacatta
    11641 ggacctaaag gaagaaatgt tatattagat aaaaaatttg gttctccact tataactaat
    11701 gatggagtaa ctatagcaaa agagatagaa ctagaagata gatttgaaaa tatgggtgct
    11761 caattagtta aagaagtagc aactaaaact aatgatgtag ctggagatgg tactacaact
    11821 gctacagttt tagcacaagc tataataaga gaaggtttaa agaatgtaac agcaggggct
    11881 aacccaatac ttttaagaaa aggaatacaa aaagcagtaa cagtagcagt agaagaatta
    11941 aaaaatcaat caagaatagt agaaacacaa gaggctatat ctcaagttgc ttctatatct
    12001 gctggtgatg aagaagttgg aaaattaata gcagaagcta tggaaatagt aggtaaagat
    12061 ggagttataa ctgttgaaga gtctcaaact atgaatactg aattagatgc tgttgaaggt
    12121 atgcagtttg atagaggatt tgtttctgca tatatggtta cagatgtaga taaaatggaa
    12181 gcagttttaa atgacccata tatattaatt actgataaaa aaatatctaa catacaagaa
    12241 ttattaccag ttcttgaaca aatagttcaa caaggtaaaa agttattgat aatagctgaa
    12301 gatgtagaag gtgaagcatt atctacatta gtagtaaata aattaagagg aacatttgat
    12361 gtagtagcag ttaaagctcc aggctttgga gatagaagaa aagaaatgct tcaagatata
    12421 gcaatactta caggtgctca agtaatatca gaagaattag gttatgattt aaaagaagct
    12481 gatttatcta tgctaggtag agcttcatct gttaaggtaa ctaaagaaag tactacaata
    12541 gtagatggtt ctggagataa aaaagctata gaagatagag taactcaaat taaacatcaa
    12601 gtagagcaaa ctacttcaga ttttgataga gagaaattaa tggaaagatt agctaaactt
    12661 gctggaggag tagctgttgt aaaagttgga gctgctacag aagtagagtt aaaagaaaga
    12721 aagttaagaa tagaagatgc tcttaatgca actagagcag ctgtagaaga aggaatagta
    12781 gctggtggag gaactgcttt tgttagtgtt ataccagcaa taggaacact aattgagagt
    12841 ttagaaggag aagttaaact aggtgctcaa atagttaaaa aagcgttaga agagccatta
    12901 agacaaatag ctataaatgc tggtcttgaa ggtgctgtaa ttgtgcaaaa tgttgttaat
    12961 tctgaagcag aaactggatt tgacgcattg aatgaaaaat atgtaaatat gatagaagct
    13021 ggtatagttg acccaactaa agttagtaga agtgctttac aaaatgctgc atcaatagca
    13081 agtactttct taactactga agctgctgtt gctgaccttc ctgaaaaaga agatgcagga
    13141 atgccaggaa tgggtggcgg aatgcctggt atgatgtaat ttaaaaaatt taaaatataa
    13201 attttaatat aaagaactat ttcgacttag attttaaact ctaagttgaa atagttcttt
    13261 tgtatatatc taatttttgt atatctataa attcaataca catatttaga taatcagtat
    13321 ctagtttgta ataatatttt agagtagtat tgttattttt taggttatga ttttagatat
    13381 atattaatta aggtataatt gttgatagag aataaatcaa atttaaaagg atgcgataat
    13441 atggacttaa gaaaattgct tgaagaagtt aaaaatgaaa atattgatat agattttgcg
    13501 ttagaaaagt taaaagattt accttatgaa gaccttggat atgcaaatat agatcatcat
    13561 agagaactaa gaaatgggta tccggaagtt atttattgtg aaggtaagag tgatgagcat
    13621 attttaggga ttattgataa aatgaatcaa aaaagttcta acatattggg gacaaggtgc
    13681 agaaaagaaa cttttctgaa aattaaagaa atctataatc atgctgagta cgaagaggcg
    13741 tctaaaattc ttaagattca aaatcatgat atagagaata taggaaaagg taaaattgtg
    13801 attgctacag ggggaacttc tgatatacca gtagcagacg aagcttatca tacagctaag
    13861 tttttaggta atgatgttga tagaatatat gatgttggag tggcaggtat acataggctt
    13921 ttaaataaaa gacataaaat agatagtgct agagtaatag tagccgttgc aggtatggaa
    13981 ggtgctcttg caagtgtaat tggtggactg gttgatgttc cagtaatagc tgttccaact
    14041 tctgttggat atggagcaaa ttttggagga ttagcagcat tactagctat gttaaatagc
    14101 tgtgcatctg gaatatctgt tgtaaatatt gataatggat ttggagcagg atatttagca
    14161 tcaatgataa ataaactgta aatacattga tataaattaa acaaaactat ataataaaat
    14221 ttaatttcta ttatattttt actattaaaa ttgaggagac atatatgagt gaaaaaatac
    14281 tttattttga tataataagc ggtatatcag gggatatgac ccttgcaagt ttattaaatt
    14341 taggtgtgcc taaggaaatt tttttggagg aaattcataa actaagaatg agcgatgaat
    14401 ttaatataaa catcagctcg aaaactgaaa atggaatagt aggtacaaaa gtagaagtaa
    14461 ttacaaaaga aaaacataca catagaaatt tagtggatat atttgaggtt atagataaaa
    14521 gcactctaaa cgaaaatgta aaatctaaag caaaaaaaat atttatggta attggagaag
    14581 cagaagcaaa agttcatgga actacaatag acaagataca ttttcatgag gtaggggcta
    14641 tagattcaat tgtagatata gtaggagctt ctatattagt tgacttactt tgtgtagata
    14701 aagtttgtgc tagtacagtg tctgttggtt caggatttgt aaaatgtgaa catggaatta
    14761 tacctatacc tgcacctgca actatagaaa ttttaaaagg tgtgccaatt aaattaaata
    14821 atgtaaatgg agaatgtaca actccaacag gagctgcaat cataaaagtc ttgtgtgatg
    14881 agtttgtaga tgagcttgaa ttttctccaa agcaaattgg atatggtata ggtcataaga
    14941 agtttgaagt accaaatata ctcagaactg tattaggtga aaaaaaaaag aagaattaat
    15001 ttatgaaatt agtgcaaaca ttgatgatat gtcttcagaa atctattctt atttgtatga
    15061 aaaaatatta gatgagaaag cattagatat ttatacagag agcatttata tgaagaagaa
    15121 tagaccagca acaaaagtat caattttatg taatgaatca gatttagaaa aatttattaa
    15181 aatattactt ttagaaacta gtacttttgg agtgagatat aatgcatata atagagaaat
    15241 tctagagaga aaatttgtaa aattagatac aaaatatgga gtggttacag taaaactagg
    15301 gtattataaa ggaaaactag tcaaggctac tccagaatat aatgaatgta aaactatagc
    15361 aaaaaatgaa gatattccta taaaaaaagt gtttgctgat ataaatttta gaataaaaga
    15421 agaatttagt gaaaacttat tgacataagt aataaatttt gataaattaa tattcagcct
    15481 tattaaataa aagtaaacag aaaaaaatat ataaaatata aactcactcg tatatgctca
    15541 gaaatatggt ctgagagtct ctaccaagat accgtaaata tcttgactat gagtgaaatt
    15601 attataccaa aagacctagt taagtaaatg tgtaggctga gttaaactcc ctatgtgttg
    15661 gctataagat aaaggtattt tggaagcctg tatagtaatt tcacttttgc tagaaattat
    15721 ggaagttact atacaggttt tttgtgtaaa aagatttaat ataatttaga ctgtattgtg
    15781 ttaaaataaa tatataaaaa ctatattata caaggagaga tgtgaattta tgaaacatga
    15841 attagtactt gtaatagatt ttggaggaca atataatcag ttgatagcta gaagagttag
    15901 agaaaacaac gtatattgtg aaatacttcc atgtactgca agcatagaaa gaataaaaga
    15961 aaaaaatcct aaagggataa tatttacagg cggtccaaat agtgcgtatt tagaagattc
    16021 tccaacaata tcaaaagaaa tatttgaatt aggagtacca atacttggaa tatgctatgg
    16081 tattcaaata atgtctcatg tattgggtgg agttgtaaga aaaggtaata aacaagaaaa
    16141 agaatatgga aaaacagcta taacttatgg aaaatcatca ttatttgaag gaataactac
    16201 aaatagtgta tggatgagtc acacagattt aatagaaaaa gttccagaag gatttactat
    16261 agttgcaaat actaatgatt gtccagtagc agctatggaa aatgtagaac gaaacctata
    16321 tggagttcaa ttccatccag aggtggaaca ctgtttggaa ggggacaaga tacttacaaa
    16381 cttcttatat aatatttgta aagtaaaagg tgactggact acagattcat tcatagaaga
    16441 taaaataaaa gaattaaaag aaaaaatagg cgataaaaaa gctttatgtg ctttaagtgg
    16501 aggagttgat tcttctgttg ctgcagttct tattcataaa gcaattgggg acaacttaac
    16561 ttgtatattt gttgaccatg gtttacttag aaaaaatgaa ggaaatgatg tagaaagaat
    16621 atttagagag aaatttgata taaatttaat aagagttaat gcagaagata gattcttatc
    16681 taaattaaaa ggagtttctg aaccagaagc taagagaaaa ataataggtg aagaatttat
    16741 aagagtattt gaagaagaat caaataaatt aggaaaaatg gatttcttag tacaaggaac
    16801 aatctatcca gatgttatag aaagtggaca tggaaatgct gctactataa agtctcatca
    16861 taatgttggt ggaatcccag aagatgtaga cttccaagaa attgtagaac cattaagaga
    16921 gttatttaag gatgaagtta gaaaaatagg attagagctt ggtatagaag aaggattaat
    16981 atttagacat cctttcccag gaccaggtct aggaataaga gttataggtg atgtaactaa
    17041 agaaaaatgt gatatattaa gagaagctga tgctgtttat atggacgagc ttagaaaagc
    17101 aggtttatac agagaaatat ggcaagcttt tgcaacatta ccagatgtta agactgttgg
    17161 agttatgggt gatgaaagaa catatgctta tttagttggt ttaagagcag ttacttcttc
    17221 tgatggaatg acttctgact ggtataagat gccttatgat gtattagaaa gaatttctaa
    17281 tagaataata aatgaagttg atggagtaaa tagagtagtt tatgatataa cttctaagcc
    17341 tcctggaact attgaatggg aataaaatat agaatgccaa aaatccacat aaatacagga
    17401 tttaaggtct ctcttacttc tatgttatac tggtttgata ctatcaatat ttgccagtat
    17461 cttatggatt aataagcaaa aaacatcctg ctgaatgata aattcagcgg ggtgttttta
    17521 ttttgtttta gaactccttt gctctgaaac tcgaagtatt taacactgat gatgttgtta
    17581 tcttttagtt ccttttgtgt catgctctga aagacaaatt gatacaataa aaaagtaatt
    17641 tcttaatcat tgatggagat atgtatttat gttactcttg atggtaattt gagtaatatt
    17701 gttcatagct ttttatattc acttaattag aaaaccattt ggtataatca taaataaact
    17761 tgatgatata atagaaaaga tttataatta ttagaagtat ataaaagtat tttatgtcta
    17821 tgaaatgtat gtctataaaa catatagatg acttttgtat attaatgtat gaatattgat
    17881 attatagaag aattgagttg tcaatgatac taaatcaaat atttatagaa tctggaggga
    17941 ttatgagaaa taaaataatt ataggaatta ctttttcact tgtattacta atgtctggat
    18001 gtgcttcaaa acaaaccgca gaaataaacg tattagcaac tactgattta catggagtta
    18061 taccttatga gttaacttcc tatgtaaaag aagaaagaaa aaaagataaa aatctaactt
    18121 tagtagatgc aggagatttt tttgatagtg gcgaagtatc tggttctagc atggatagtt
    18181 attttagtgc taggtataag gatattgata ataacactga gcgttacatt gagacaccta
    18241 tttcaaaaga tatgaaagag gtaggttatg atgttgcagt tcttgggaat catgaattta
    18301 tatccaacaa taaattttat ctagataata tgatatctga ttttgagaaa caaaaaataa
    18361 atattttatc ggcaaatacg tataaaaaaa atggagatag ttatacaaaa ccatacataa
    18421 taaaaaatat taatacacct gaaggaaacg taaaactagg aattttaggg cttactataa
    18481 aagaagttgg tgagaggaaa cagtggaaag atggaaaact tgttgactct aagtctttag
    18541 aactaaaaga ccaagagggt tacaatggtg aattgtatat gaatgattta gtagaagatg
    18601 ctaaaaagtg ggttggtata atggaaaaag agaagcctga cattatagtt gctgtggcac
    18661 atacaggtga gaaacctaag aaacctaaaa acccaggaaa tagaatacaa gacttagctc
    18721 aacaagttga tggaattgat gctatagttg caggtcataa tcatgttcaa attgagcaac
    18781 atgattataa aaataaatca ggagaaaatg ttatagttac tgagccagga aagcatgggg
    18841 aatgtatttc taagattaat tttaaattag aaaaaaacaa tgataaatgg aatatagttg
    18901 acaaatctag taaattaatt aaatttgaaa aaaatcaaga aagtgataaa tttggagaat
    18961 ttatttcttc aattaataaa gtaagtaaag ataagaaaga aataagatta tcagaagtaa
    19021 agactcctga atgggataaa atatatgttt ttaaatctgg aacatctgca gatgatatat
    19081 ataaaacaat aggatataaa tggagaaata tatcatctcc aaagggtgat gacatggtgc
    19141 agattgttgt aatgaataat aataagcctg ttagctatgt atatggtgaa gctgaagtta
    19201 tgccaattaa tataaaatgt gatgaatcag agtataaaga taatgttgta actatttatc
    19261 ctaataaaaa tgataaattt aaaattgaaa aaggtaaaca agaatatagt attaatttaa
    19321 aacatataca atagaaactt ttatcttatt taagtttaga ttgtacttaa aataaagtgt
    19381 gtattatttt aaaaattgat gataattaaa atcacaacat taaggaatag tacacacttt
    19441 ttatgctaaa aaatagaatg ttaaaaaatt aaacctttaa attaaagtac attgcttata
    19501 ttatccaata ttatcttcaa aggttgtgtg atatttttat ccttatgtaa tattgctaac
    19561 attttatttt ccatatctat cccatctaat ttaatcaatc gcaatttttt atgaaagagt
    19621 gaatctaata ataaattttc tggcaaaatg gttataccta accctgcttc tgcagcttta
    19681 ataagtgcaa aagaattaac actttcccaa acaggataag ctttttggtt tgaaagagat
    19741 aatacactgt ccaaagtatc gcgaatggca cttccttgct cacgtaacaa taacggaaac
    19801 gtgcataact gtcgtagaga taaggcttgt ttagataaag gaaaagttgg agaacatgca
    19861 acacataact tataggaacc taataaaatt gtacggaatg ccccctgtgg ttctatacct
    19921 tcccaaaaac atatatcagc ttctccactt tttaaaatat ccatagctga attagcactt
    19981 tcgacacgta cattcacttt aagagatggg aatgaatttt ttagtcgact taaaattggt
    20041 ggaagtaaaa aagaggcaat tgtaatgcta gaaacaatgt ttataggtgt acattcctct
    20101 aaacgactga ttcttttgtc taaattacga caagcagtta agatactacg tgcttcttct
    20161 aaaagagaaa tgccacagcg agtaagacat acccctcgtg gtagtctgtc aaataatgct
    20221 gtttcagctt gttgttcaag ttcagctata gcatgagata cagcagattg tgtgatatgt
    20281 aatttttttg cagcacctgt aaaagtgcct gtttgttcaa ttgcttctaa aatctccaaa
    20341 tatcgtatgt ttatcataat aaatatcctc actagacatg aataaaattg attaattcca
    20401 ttaaattcta tcattttaca gattacaaag caacaggtat actgaataat atgaagtgaa
    20461 aagaaggagg aatattttga atcaaagcag aaaaacatat ttttggcttt taattattaa
    20521 cttgtttatt agcacattca cttttggtgg agggtatgta gttgtaccta tgattagacg
    20581 ttattttgta aatcaaaagc aattctttac agaagatgat ttgatgagca tggcagcagt
    20641 tgcacaatca actcctggag ctattgcaat taatttatct gctttggcag gttatcgagt
    20701 agctggtact ttaggggttg tcattagttg tatagcagct ataatacctc cactcgttat
    20761 tcttggatta gtatcagcat tttacaccac ttttatatct aatgccattg taactgcagt
    20821 attaaaagga atgcaagctg gtgttgctgc actaattgtg gatttgatta ttgacatgtg
    20881 ctctatgata ttaaaacaac gttctctatt tttatcagct atgataccta ttgctttctt
    20941 tgcaaacttt gtgatgagta taaatgtaat ttttatatta tctgtttgct gttttgtatg
    21001 tgtaatgcaa gctttctaca agaagaggga aaataaatga atacaatttg gaacttattt
    21061 tttatatttt taaaaatagg attgtttagt attggcggtg gttatgcaat cataccttta
    21121 atccaagaga aagtagtaaa tcaaactgga tggataagtg agaaaatgtt tacagatatt
    21181 ataactattt cacaaatgac accagggcca ctggctgtta atacttctac ttttgttgga
    21241 cttcaaattg gagggttttg gggagcagtt gcagccactt taggttgtgt attatgtggt
    21301 gtagtaatat ctctattttt atataatttt tttcaaaagc atcagaactc tacttatata
    21361 tttgaaatat taaatggtct aaaatcagct tcattaggtt taattatctc agctgcggct
    21421 acaattataa tccttgcatt atttgggacg aataaagtgt acttcaattc tatatttgag
    21481 atgctaaact ggactgcact aataatcttt tttgttacac tactgatagt acgtaagtgg
    21541 aaaattaatc ctattgtgat tataataata agtggtatac tgggaatatt tttttattta
    21601 taagtttttg ctaagtaaag gatatctatt tttttatatc ttaaatccaa atagccaata
    21661 aaataaggct atttggattt ttaataaatt ctgaaaagat aatcagaaac ccatataaca
    21721 tttaaattat atcactggtt ttctacaaac caatcataag cttcttggca aaatttctca
    21781 ctatctatat agtaactatt atgaacatta ttaatttcaa caagtttctt tttacatttt
    21841 agtgaattat aatagctatc aaaatattgg gttttaaaat atctatcatg aagtgtttta
    21901 aagacaagag taggtatgtt gactccatct tttatcttac tatcttttgc aataaagcat
    21961 tctaacattg tattaattat ctttgtacta tttttccaaa tatgaggatt tttcttttca
    22021 aatattttat aaaattcatt atcatagact tcattataac ctttatattt aggtggtttt
    22081 actgttatgt gaaaattagg aagaaactta aagaaaaaat ataataattt ttatgttctg
    22141 ttttatatat tattcatatt ttatcacaga taatatacta aagttaattt ttttattgta
    22201 caactattat aataattctt ctataatata agacattcta taattttata tttaagttca
    22261 tgtgtattta attgttttta tataaaatgt ccagtaatac tatttggatt tatttttatt
    22321 tcatcaggag ttccagaagc aacaatttcc cctccattta ttccactgtc tggacctata
    22381 tcgataatcc aatcagaagc acgtataacc tctgtattgt gttcaataat taaaagtgaa
    22441 tgctgttcat ttacaagttt tctaaaagta attaacaaat tttctatatc tttaaaatga
    22501 agtccagtag taggctcatc caatatgtac agcattcgtt ttttagtttt tgtccctaat
    22561 tcttttgcta attttatcct ttgagattct cctccactta atcctaatgt attttgtcca
    22621 agctttagat atccaagtcc cacttcatct aataaggaga gttttctata tatatcagag
    22681 caatcgctaa aaaatgtttt tgcttctgct acttccatgt caagtatatc tgcaattgtc
    22741 tttcccttgt attgtataga cagtatttct ttttgatatc tcttaccttg acattcacta
    22801 caagttaccc attggtctgc catatattgc agaataattt ttgttttacc taatccttga
    22861 cagtttgggc attgcccctt tttgctatta aagctaaaat agctttcatc caataatttt
    22921 tgttttgctt gtggagtttc agcaaataga gaacgaattt catcaaaaat attgatatat
    22981 gtagcaggtg tagaacggct agattttcca ataggtgttt ggtccattaa gataaaatca
    23041 tcaatagcat caaatccagt aaattcagta taatttttat ctgggataga tttttgtttc
    23101 aatttttctt ctagagcagg aagtaaactg tgaagtacaa gagagctctt accagaacca
    23161 ctaacacctg taatagagca catacagttt aagggaattt ctacatcaat attttttaag
    23221 ttatttgcat ggcatccttt taagcttaac catttacttg gtatagtttt attttgtgaa
    23281 cctgttttat tgtatgtact taaatattta cctgttatag agtttggatt ttttataatt
    23341 tcttgggttg taccttctgc aataatgaac cccccttttg taccagcact tgggcctaca
    23401 tcaataatat aatcagcaga cagtattgta tctttatcgt gttccacaac tataacagta
    23461 ttacccttat ctcttagctc ttctatcatt ttgatgatta aattatggtc tcttgggtgt
    23521 aaaccaatag atggttcatc taaaatgtag gttaagccta ctaattcact tcctagctga
    23581 cttgataatc gtactctctg cagttctcct ccagataaag ttggagctgt tctatctgtt
    23641 gatatatagc taagacctac ctttaataga taactaactc gaatttttaa ttctgttaag
    23701 atatcattta ccaaagagag ctcatttttt tgaagttgat ttggtaattc acacaaccaa
    23761 ttccataact gttctattgt catttttgtt agttcaggga agcgataacc tttgattgta
    23821 gtgaatctag cctcaatgca cagcaattcg ccaccacatg tattgcaagg aattttgttc
    23881 atatattgat gcaaggtatt attttcagaa ctagattctc taaaaagtct ttgaatgtgg
    23941 ttgattgctc ctgaagcagg tcttctaatc tttgattctc gtccctttga ttcaaagcta
    24001 aattcatata tcttatcgtc tgtaccatat aagattgcat ctataaaatc tcttggtaat
    24061 tcattccaag gtatatctag gtctatattt ttatcttttg ctattgcata taattcacct
    24121 accatccaat taccatttgg ctttttcccc ctcaattttc caaaatagta agtagctcca
    24181 tctaatattg aaatagttgg gtcttttaca attaattgag gattaacttg ataggtaaaa
    24241 cctaatccat tacaatcgtg acaagctcca gtatgggagt tagcattgaa ggttgaagta
    24301 tttattttag aaaaaatagt ttgacaatgt ggacaataat tttttgtatt attctttttt
    24361 tctaatggaa ttttgcaata tggacagtaa ctttttctga ttgatacaaa taacattctt
    24421 aaataatccc aaatgcttgt aagagtacca acagtagagc gaggattttg gcttcctttt
    24481 ttctgttcga tagctatagt aggggttaat ccctgaagta aatcaaattc cggtttttga
    24541 agctgttctg aaagttttgc tccattactt agattttcaa aataacgtct ttttccttct
    24601 tcataaatcg tatcgaaagc taaagaagat tttccactac cacttacacc tgtaataaca
    24661 gttatttgat tttttggaat tgtaacctca acatccttta aattgttttg ccttcctcct
    24721 tttaaataga tagagctttt attatgacaa agtgaatttt taattgattt atttattgct
    24781 tttgtaaatt tgaatttctt ttctagttca agaggattgc ctaattcttt tatttccgaa
    24841 atacctaata tttttaactg agaaaagatt gatttcaaat aatcttcagg caatttttct
    24901 attaacaaat taaggtcttc tatttttata tttccgcttg ataatatctc tgccatttca
    24961 tctaataaat ttttttctgc tcttgttaat tgattgtatt ttttggaact taattctgtc
    25021 cataaatccc atggaagcaa ttctaaacca atcatattat taaagtcatg tagaagatta
    25081 ccctttacag aatgccaacc tttccaattt ttattatatc caaagtcatc tggatgagca
    25141 tgtccttgcc gacatcttat ccaggcttct gaaccagtta aaaaaccatc tgtttttccg
    25201 acatcatgag tgtcaaaatt aatttgaaaa taattttttt gtatgtcatc tatttgtgca
    25261 tctattctta tccatctctt tgttagagta tcatagtatt ctacaagcca gtggtcttca
    25321 taagtaagct cactttcaaa ataattagca aaacctgctc tcattctagc ctcaaaacct
    25381 cgatatctta ggagcgatac aagaagtaaa gaataatctc tgcacattcc aacaagcctt
    25441 tgttttgggt ttcttggaat agttagtggg ttagaattta tttcaaatat tctttttaac
    25501 atttgaggaa ttgtacgaag caattcctca tcagattgtt gtttattaaa ggatatacca
    25561 tataattttc cttggtctcc atgaattact aggctttgaa ccacttttaa taattcaatt
    25621 ggagtctttg gtaaatcttg atatttattt tcaaattttt taggatttgt gatgatgctt
    25681 tgatttttat agttatttat tttcatttat atttcacctc tctaaatgga tgatataccc
    25741 tgacatggtg tcatatgcaa ggacaattta gattttttgg ccaaaattta aaaataaata
    25801 aaaaatgata cagtgataaa ttgtaataca aatatagttt caaattgcat ataatataat
    25861 tatttaataa aaccattatc tatggaatgt taatgaaatg ttaagatggc attcttgaat
    25921 attaagaaat agaatactat aatatagtgt gaaggattct tagtaaaaaa atttgtaagt
    25981 gttaatatac attaaaaaat aggtttataa tttggaggaa aaaaatgaaa ttatcaaggt
    26041 ataagaaaaa tagtttagtt ctgtctataa ttttatgttc acttattatt tcaatccttg
    26101 taattactgt ttttaatacc gtaaaactac acaatatatc taataaacaa tctaaaaact
    26161 atttaaacga tgtatctaca cagattgtaa tgaatgttga ttctaaaatc aaatttattt
    26221 tatccgactt aagaataatg gcagatttta ttaagcaata tgaaggtgat agtcgatatg
    26281 agtatttatc taaaagaaag actagttata agtattatga tgttggaata atagatttaa
    26341 aaggtaaagc tgagttttta agtggaaaaa agtttgactt aaagaatact gcttcctata
    26401 aaaaaggaat tgaaggtaaa gaatactcag aagtaataga gtcaatgggt tttgttcttt
    26461 atagtgtacc tatatttaat gataatcatg aagtatctag tattttaata ggtgtatctg
    26521 ataaagaaag tatgaatgag attcttaata tagataactt taatggaaaa ggaactattg
    26581 aaattattaa tagtaaggga aaacctcttt tcataggaaa aaatactgaa ttaataaagg
    26641 accttagcca taaatataag agttctggag atgaaccttg ggcacaaaag atgcttgagg
    26701 attttaaaaa tgataaatca gggaatatta caataacctc atcaaaaggt gtacaatgct
    26761 tattaactta tcatccaatt acaagaggtt taaatgattg gcattttctt ctcatagtac
    26821 ctgaagatgc tgtacttggt gaacttaata aattaaactc ttttacaata acaatgacat
    26881 ttataattac atgtattatt ggaattataa cattaatttt atatattata agaagaaaat
    26941 atgtaaatca aatagaaaat attgcatatt tagatagtat aacaaatggt ataaattcaa
    27001 ctaagtttag tatgctagtc aaacctttaa tttcagaatc acctgatagt acttatatga
    27061 tgatagcaat gaatataaaa gattttaagc taataaatga ttgtttcggt agtgaaaagg
    27121 gaaatatgac actaaaacat ctttacaata ttttgtataa aaacattgat gaaaatgaag
    27181 agtttgtgtg tcgtcatgat gcagaccttt tctatttgct tattaagaat agaccagtgt
    27241 tagaagccct ggagttatta tataaaatag aagctgacac aaattatttt aataaaaata
    27301 gggaaaatcc atacttttta agactatcag taggtattta cacaattgaa aatcataatg
    27361 aagatttaat tacaatacaa gaccatgcaa atacagcaag aaaaagcttt aataaaactc
    27421 atcaaagtga tttttctttt tatagtgata tagaaagaaa acgattaata tctgaaaagg
    27481 aaatttcaaa tttaatggaa aaagcccttg aaaataaaga attttttatg tgcttacaac
    27541 ctaaaattga tataaaaagt ggaaaaatta gtggagcaga gtctcttgtg cgttggaaaa
    27601 attcagaaaa aggcataatt tatccaagtg attttattcc tcttttcgaa aaaagtggat
    27661 ttatatgtaa gttagattta tatatactag aagaaacatg caaattgatt tctaagtgga
    27721 taaaagaagg taaagaagtg ttgagaattt ctgttaatgt atcacgtcaa catctgaatg
    27781 ataaattgtt tttagaaaaa tataagcata tatgcaataa atacaatgta ccaacatgtc
    27841 tgatagattt agaacttacc gaatctattt tcttagaaaa tccagaagct atagatataa
    27901 tagaggatat tcattctaat ggatttaagt gttctataga cgattttgga tttgggtact
    27961 catcattagg aatattaaag gattttaaag ttgatataat taagttagac cgttcattct
    28021 ttgttagcaa aaataatata gaccgtggta aagtagttat taagtctatt attgaactta
    28081 gcaaaaggct aggtatgaaa gtaactgctg aaggaattga agaattagaa caagttgagt
    28141 ttctaaaaaa tgtgggatgt gattatattc aaggttatgt tttttcaaaa ccattattaa
    28201 tttcagactt tgaaaatttt gcatatgaaa ataataaaat taaaaatatc cattattaaa
    28261 tgtacaagat aaaaaattta ggcaagttat ataattaaaa atataacttg tcttttttat
    28321 ttatatattt aataaaaata tatttgcaga ttttgtaaaa ataattttca aatgttgtaa
    28381 gttgtggcta aagctttatt gtattattct gaatatagaa agatagttac tgtcttagat
    28441 aaaaactgaa acatacataa taatataaaa tttgctatat taataactct aattattttg
    28501 gaaggaggga aaatatgata agtgggagaa tgttactcat catgaatttt ctaaaaggaa
    28561 aaaataagac aagttacaag gatattagca aacaacttag tatagaagaa agaaaagtta
    28621 gatatgatat aaataattta aatattgttt tgcgttcatt aaacaagtca gtaatacaaa
    28681 agatgaacaa aggtgtgtta attattcctg atgacttcca agtagtatgt ataaataata
    28741 acgaatatgt attttcctta actgagcgag tatctattat gaaaattata tcaatgttta
    28801 aaatagataa tctaaactta gaaaaattaa gtaaagaatt tcaggtttct agaagttcaa
    28861 ttaaaaatga ccttaatgtt ttgtcaagtg aattagaaag aaaccaaata actattactt
    28921 atgataaatc ttttaaaata agtggagaag aagacattat atataagcaa agattgaaca
    28981 tattaaaatc gtattcttat ctttttggta aggaaaaatc tgatttaagt gtatttgaga
    29041 agtatttagt gaatgtaatt gaaaaaatat tcaaagggat ttatttgatt gatatatata
    29101 attggtctat gaatttatta ttgcaaatgg gatggacttt aaatgatgaa tcttttacat
    29161 ggtatgtgtc gaacattttc ctttttacat ggtatataca ttcagatgaa aaacatcctt
    29221 tagaatctgc ttcattaagt gagccgtgtt ttgaaaataa gtttgaccaa gaatttgaaa
    29281 caattattca aaaaaagttg actaaggaac aaatttttct gttaaagagt tttgtatttt
    29341 ttacaaataa atatgcaagt ttaaacgagg atatggattt gattagtaca gaaattatag
    29401 ttcaagacct aatttacaat atgtcagaaa tattagagat tagctttaaa gaagatatga
    29461 ttctttataa aggattactc aatcatattg ccccattgat agagagaatc aagcagaata
    29521 tacagattta tgaggaatta cttcatgtaa taccagataa atatcaatat gtacttgaag
    29581 ctactaaggt ttctattcaa aaaaacagag ttttaagtca aataaagaat gaaaatgaaa
    29641 caatactttt ggcaatacat ttcttaggaa gtatacatag aaatgagagg aataactaca
    29701 aaaatatatt gttagtttgt ggatttggat atggtgtaat tgctatgtta aaggatagtt
    29761 tagtaaatga ttaccagata aatatagtag agagtattcc atcatataag ttacaagatt
    29821 ataaagactg ggaaagtatt gacttcatta taacaacatc aaagatagca atagacttgc
    29881 ctaaaacaat tattgagata aatccatttt tacaagaaga ggattatttg aagtttgaag
    29941 aaaaagggat aaaaagaaag aatgtcttaa ctaattatat gtcaataaaa aaaaggttgg
    30001 actttttaga agaagatgcc caaaaaagag tattaagtat tatatgtcaa gaacttggat
    30061 acagtgatga gagaatatta tttagacaac ttaatttaag tgatttaata gggattgata
    30121 ccataaggat aattgatgaa gatatgaaat gggaagatgc tgtaaaattt agttcaaaca
    30181 tacttgctaa ttgtgatttt gtatcagcac aatatgctga gaatattata gatattttaa
    30241 aaaatgtggg tttttatgct gtaaaagata acgaatttgc tttactacat ggcaatgata
    30301 gttcattagt caaagtaagt tcgataagtt tattaatatg taaagaagca gttagttttg
    30361 gagataaaaa ggttaaaatc atattttgcc tagcaagcag agataaaaaa gagcaaattc
    30421 ctgcaataat taatctaact aaaatggttt ataagactaa ttttattgct atgttagaaa
    30481 gtgcaaagac aaaagaagaa gcagaagttt taattcataa atatgagaag gaggtcactg
    30541 tatgaataaa ttagttaatg taaaatgttc atttaaaata catgttaatt caaaagaaga
    30601 agccattata tcgttagttg atgtagtgtc aaaagaagga tatttaatag ataaaaatca
    30661 atttttaaaa gatgttttaa agagagaaga aacattgtcg acttatattg gacatggtat
    30721 aggtcttcct catagtcaaa gtgtaggtgt taaaaactct tgtattacaa taggaaagct
    30781 tgatactcct attgaatgga cagaagaagg agaaaaagtg gatttaatat ttttgatatc
    30841 agtaacaaaa gacaatgaaa ataatcttca tttaaagatt ttatcaaaat tagcacgttt
    30901 attgatgcat gaaagtttta gaaatcaaat tagatgttca gatgaacaaa ctgtttataa
    30961 tcttataaaa gaaaaaatag aggaggaaga ttaaaatgag aattttaaag tttttgcaaa
    31021 aacatcttat gacagcaaca tcttatatga ttccatttgt ggttgcagga ggaatattat
    31081 ttgctttatc agtaacttta agtggacaag ctgctgttcc tgaaacaggc tggttggcta
    31141 aactgaatca aattggagct gctggcttgg ctttattcat tcctatatta ggtggatata
    31201 tagcatttag tatggcagat aaaccaggat tagctccagg tatgataggt gcatatttag
    31261 caaaagaagt aaatgcaggt tttataggtg gtatcatagc aggatttata gcaggatttg
    31321 ttgttttaca attaaagaaa attaaattag ctcctacaat gagaacttta agttctatat
    31381 ttatttatcc tttgattggt acattgatta caggtggtat aattgtattt ttaattggag
    31441 aacctatagc ttcttttatg acatggatga caaactggtt aaatggaatg tctggtgttt
    31501 ctaaaattcc tttaggtgga atcttaggag gaatgatagc ctcagatatg ggtggtccta
    31561 taaataaggt tgctgctact tttgcccaaa ctcaagttga cacacttcca tatttaatgg
    31621 gtggagttgg cgttgcaatt tgtatacctc caattggttt aggattagct acattattgt
    31681 ttccaaaaaa atttagcaag gaagaaagag attcaggtaa agcttcacta ttaatgggat
    31741 gtgttggaat tacagaagga gctattccat ttgcaacatc agaccctgtt agagtaatac
    31801 cttgtattat ggctgggtcg attataggaa atattatggc ttttttatta ggatgtttaa
    31861 atcatgcacc ctggggaggt ctgatagtgt tacctgtagt tgacaatagg ttggggtata
    31921 ttgcatcagt attaactgga gcagtagttg ttgcggtatt aatgaaatta gtaaaaaaag
    31981 acgtgaaaga agatgaagaa attgaagaag atttagatga atcaattgaa ttaatatttg
    32041 aagaattata aatataaatg gaaggaattt gtataaaaaa gtaaatataa aactataaat
    32101 ttaaaggaga taagaatatg aaaattgtag cagtaacatc ttgtcctagt ggagtagcac
    32161 atacttacat gtcggctgaa tcacttgaat tatcagctaa aaaatttggt atagaaataa
    32221 aggttgagac tcaaggaagc tctggaatag ataatgaatt gtcacttaaa gatattgatg
    32281 aagcaacttg tgtaatattg actaatgatg ttgagattag aaatatggaa cgttttaaag
    32341 gaaagaaggt agtaagaatg agtgtatctg agataattaa gaaatctgat gcattaatta
    32401 agaaaataaa agatactttt caataaaaat tgatttattt tacatataac tcaaagatgt
    32461 ttttaaagaa atttttatta aaagcatctt tgaaaatatc ttaaaaatag aataaaagga
    32521 gatatagtca tgaaaaagtt accaataaag actgtcgtgc aaagattact aaacttacaa
    32581 gaagagggga aaagtgcaac tcttttagga attggtccta tgtcaccaaa cttgttacaa
    32641 gcaagttttg aactagcaaa agatgatgat tttcctttaa tgtttatcgc aagtagaaat
    32701 caagtagatg cagatgaact aggtggcggt tatgtaaatg gttggaatca ggaaactttt
    32761 acaaaagata ttaagaaggt tgccgacaaa gttggatttg atggtttata ttatttatgt
    32821 agagaccatg gtgggccatg gcaaagagat aaagagagaa atgaccattt acctgttgat
    32881 gaagcaatgg agttaggaaa aaaatcttat ttagcggaca ttgaagcagg ttttgaccta
    32941 ttgatgattg accctacaaa agacccattt gaaattggaa aagtaattcc actagatgta
    33001 gtactagaaa gaactgtaga attaattgag tattgtgaaa acgagagaaa aagacttaat
    33061 ctaccagaca taggttatga ggttggtaca gaggaaacca atggaggtct gacttcaact
    33121 gagacgtatg aaacatttat aactagacta aaggttgaac tcgataatag aggtttgcca
    33181 atgccaacat ttatagttgg tcaaactggt actttaacta gaaagacaga acaagtagga
    33241 acttttaatt ttagaaatgc ttatgattta gctcaaatgg ctaagaaata tggagttggt
    33301 ttaaaagagc ataatggcga ttacttagat gatgtaactt tattagaaca tataccatct
    33361 caaattattg ctacaaatgt agcccctcaa tatggtactg aagaaactag ggcatattta
    33421 aaattagcag aagttgagtg taaattagaa aaagagggct tagttgagaa aacttctaat
    33481 atcagacatg ttcttcttgt taatgcaatt gaatgtggac gttggagaaa atgggtagta
    33541 ggagaacaaa aaaatctaac tactgaagag atatttaagg atgaagtttt atctaatgaa
    33601 atactagata ttgcaggaca ttatacattt aataatgatg atgttaaaaa agaaatagaa
    33661 gtgctttatg ataatttaag taagaataat attgatggtc aaagatttgt tgtagaccat
    33721 attaaacgtc ctttgcgtga ttatgcagaa tgctataact taaaaggtgt tactacgagg
    33781 attttaaata aataatagga cttatagtgg tgagtattgt tatactaggt atttatagtt
    33841 ataattatac ttaccactat taaatttagg aggactaata ttttgattaa tttaattttt
    33901 gatgtagatg atactttata taatcaatta acgccttttt acactgcata taataaagtg
    33961 ttttcttcaa taaaggatat ttctattgaa gatttgtata tgagtagtag aaagtatagt
    34021 gatgaggtat ttcatatgac tgaaaatgga gaaatgccaa tcaaagaaat gcatatttat
    34081 aggataatga aagcttttga ggagttggga aattctatta cagaaaaaga tgcacaaagt
    34141 tttcaagatg agtatatata tcaacaatca caaattacac taataccaga agttgagagg
    34201 attttaaatt tttctaaaga aagaaatatt aatctaggag ttataactaa tggcccatca
    34261 aaccatcaaa gaatgaagtt aaagcagtta aatattgaaa actgggttga taaaagtaat
    34321 atttttattt ctagtgaggt tggattttct aagccagata ctaatatttt tagagtagct
    34381 gagaatgtta tgaatttaga tagagaaaat acatattatg tgggtgattc ttatagaaat
    34441 gatgtattag gtgctaaaaa agctggttgg aagagtatat ggttgaacca tagaggacat
    34501 gaagtagaag agttgtttta taaacctgat tttgtaattt tggaacataa ggatttgata
    34561 tctttattta taaaaatat
//
LOCUS       DAEMHW010000019        33383 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEMHW010000019 DAEMHW010000000
VERSION     DAEMHW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.019"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            237..1211
                     /locus_tag="KPK67_000792"
     CDS             237..1211
                     /locus_tag="KPK67_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457793.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(1327..2388)
                     /locus_tag="KPK67_000793"
     CDS             complement(1327..2388)
                     /locus_tag="KPK67_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG2457794.1"
                     /translation="MKKIRKLTTKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTRKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(2999..3850)
                     /locus_tag="KPK67_000794"
     CDS             complement(2999..3850)
                     /locus_tag="KPK67_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2457795.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(3867..4787)
                     /locus_tag="KPK67_000795"
     CDS             complement(3867..4787)
                     /locus_tag="KPK67_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBG2457796.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFAMICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(4897..5811)
                     /gene="murQ"
                     /locus_tag="KPK67_000796"
     CDS             complement(4897..5811)
                     /gene="murQ"
                     /locus_tag="KPK67_000796"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBG2457797.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(5910..7007)
                     /locus_tag="KPK67_000797"
     CDS             complement(5910..7007)
                     /locus_tag="KPK67_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG2457798.1"
                     /translation="MGRLGISIYPEKTASKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEY
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYSGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(7240..8586)
                     /locus_tag="KPK67_000798"
     CDS             complement(7240..8586)
                     /locus_tag="KPK67_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2457799.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATLLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGIEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(8675..8983)
                     /locus_tag="KPK67_000799"
     CDS             complement(8675..8983)
                     /locus_tag="KPK67_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2457800.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(9245..9643)
                     /locus_tag="KPK67_000800"
     CDS             complement(9245..9643)
                     /locus_tag="KPK67_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457801.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(9645..10805)
                     /locus_tag="KPK67_000801"
     CDS             complement(9645..10805)
                     /locus_tag="KPK67_000801"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBG2457802.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQASPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKVIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(11236..12255)
                     /locus_tag="KPK67_000802"
     CDS             complement(11236..12255)
                     /locus_tag="KPK67_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2457803.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLTSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(12249..12929)
                     /locus_tag="KPK67_000803"
     CDS             complement(12249..12929)
                     /locus_tag="KPK67_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2457804.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(13081..14988)
                     /locus_tag="KPK67_000804"
     CDS             complement(13081..14988)
                     /locus_tag="KPK67_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2457805.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLIAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPIIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(14989..15741)
                     /locus_tag="KPK67_000805"
     CDS             complement(14989..15741)
                     /locus_tag="KPK67_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2457806.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(16059..17210)
                     /locus_tag="KPK67_000806"
     CDS             complement(16059..17210)
                     /locus_tag="KPK67_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2457807.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINLLLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYGLIVLLNYLQ"
     gene            complement(17213..17680)
                     /locus_tag="KPK67_000807"
     CDS             complement(17213..17680)
                     /locus_tag="KPK67_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2457808.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGKE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(17694..18182)
                     /locus_tag="KPK67_000808"
     CDS             complement(17694..18182)
                     /locus_tag="KPK67_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457809.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIRQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(18196..19527)
                     /locus_tag="KPK67_000809"
     CDS             complement(18196..19527)
                     /locus_tag="KPK67_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG2457810.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(19541..21106)
                     /locus_tag="KPK67_000810"
     CDS             complement(19541..21106)
                     /locus_tag="KPK67_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2457811.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALEKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFVAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(21275..22060)
                     /locus_tag="KPK67_000811"
     CDS             complement(21275..22060)
                     /locus_tag="KPK67_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2457812.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(22227..23426)
                     /locus_tag="KPK67_000812"
     CDS             complement(22227..23426)
                     /locus_tag="KPK67_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBG2457813.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYIDTLEKHKALIIDIRGNLGGSDSYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKQYTNARNIKLNPVENLPTSVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(23948..25285)
                     /gene="xylA"
                     /locus_tag="KPK67_000813"
     CDS             complement(23948..25285)
                     /gene="xylA"
                     /locus_tag="KPK67_000813"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBG2457814.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIRLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(25381..26931)
                     /gene="xylB"
                     /locus_tag="KPK67_000814"
     CDS             complement(25381..26931)
                     /gene="xylB"
                     /locus_tag="KPK67_000814"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBG2457815.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKNCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            27139..28299
                     /locus_tag="KPK67_000815"
     CDS             27139..28299
                     /locus_tag="KPK67_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG2457816.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(28455..28859)
                     /locus_tag="KPK67_000816"
     CDS             complement(28455..28859)
                     /locus_tag="KPK67_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2457817.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(28880..29362)
                     /locus_tag="KPK67_000817"
     CDS             complement(28880..29362)
                     /locus_tag="KPK67_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2457818.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(29382..30191)
                     /locus_tag="KPK67_000818"
     CDS             complement(29382..30191)
                     /locus_tag="KPK67_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2457819.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(30184..30963)
                     /locus_tag="KPK67_000819"
     CDS             complement(30184..30963)
                     /locus_tag="KPK67_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2457820.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(30963..33041)
                     /locus_tag="KPK67_000820"
     CDS             complement(30963..33041)
                     /locus_tag="KPK67_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HBG2457821.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKIYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
BASE COUNT        10955 a         5875 c         3596 g        12957 t
ORIGIN      
        1 tttcatttgc tgcttaaaat caattttaag ttttcttcct ataataaact tcctctttgt
       61 ggtctcatgt tagtctctaa atttaaaatt ttacaaaaaa ttttcataaa ataaaattat
      121 attgattgta tctaaacaaa tagggcaata aaataattaa gtatagttac ttacctttca
      181 agcaatatta gttttatgtt agaattttat tatattgatt tacaggaggt ttgcatttga
      241 ataatattaa ttcacttaaa gtaataaaat tatctcttgg atgttctctt gctatattta
      301 ttgcttggct attaaaatta gaatattcaa tggttgctgg agttatagta ctacttacag
      361 taaaggacac taaaagagaa actcttaaag gctccattgg aaagatttat ggttttcttt
      421 tatgtactat tttttcatat ttatgtttta atattttagg atataatttg acttcatttt
      481 caatatttat atttataatt ataccacttt gttttttgtt aaatattcaa gatgttattg
      541 ctatgtgtgt agtcatagca tcccattact accttcaagg agaaacttct atcaaatgga
      601 ttttaaatga agctggaata tttgcagtag gaactggaat aggtgtctta ataaatatgt
      661 atattccaac aaatatacat aaaatccatg aagttcaaaa aaagttgcag gaagaagtga
      721 gtattgtact tatagacatt gcagatatta ttgtaaatcc taaaaaagaa aatggatata
      781 gtagagattt atatacatta aattcactta ttgacagcag tatttctgaa acttacgata
      841 atattaataa tactttatta agtgatacta gattcttctt agagcatatg gatattataa
      901 aaagtcaacg ggatatctta gaaaatttgt atagttatgt atctcaacta aattctacac
      961 caccacaagc tcatattcta tctgctttta tacataagat aggttatact gaatttgaag
     1021 ctgaaacagg caatcttctt cttgaagaat taaagagact aatgcttagt atgaaaaacc
     1081 agcctctacc tgttgacaga acagagtttg aaaatagagc tattttattc ctatgcttga
     1141 ctgagttaaa acagttttta gttaatcgta aacatgctca gatgcttaga gataataatt
     1201 tttataaata aaaaggggag aaatctagca gttgacctga ctacaaaata tcagatataa
     1261 tattaaatgc gcccctagta agagggcgca atgtttataa cttattctac tgtcatgatt
     1321 aaatcattat tcatatcgaa catacattat ttgaccaagt ccaatattag ctttttcatc
     1381 agtgatttct attttaatag tcacatcatt ttttttacag tacttcgcta attctgcacc
     1441 agaacgattt ttaccactaa aatctaaaga aaatggctta tcatatttaa aaggatattc
     1501 aacaggttta ccctctggtg ctaatgcaaa catcccatat ggtgtgttca taaatcctat
     1561 tacagcaatc gtaagatttg atgggtctaa atttgattca ctacctgtaa atgtaattgt
     1621 atttactgat gaattaacta cactacatcg aacaccttca tacattacat gtttttcatt
     1681 tatatcttct cctttttttc caaaatttcc aacagccttt gccccttctt gatgtggtaa
     1741 catagtgtgc ttcccaggtg gtgctggttt ccgtgtctgt tctggtttta ccgtctgtac
     1801 tattgcacca acttttggat atgctgtctt atcaaatggt aatgggtggc taagactctt
     1861 gtatatgata tcctttcctg ctgatttatt agcatcaata tctacatgat acaaacgccc
     1921 atcaccaaaa cggataagat tccactggtg gtcatttgat gtgctgtgta cataataaga
     1981 ttcaattccc atagcatcac acaaagcttt catgctccta gccattcctg agcatgcacc
     2041 ttttttagta accaatgtat aggcagtatt tgctccctct actgcattcc agtcaacata
     2101 tgttgtgtta tttttaatgt gattgtaggc aactaaaatc ttagcatctg tggtcatatt
     2161 ctttgtgatt ttcttgttca taaaatctgc tgttgcttgt gctactgagt ttagttgggc
     2221 ttgtgacagc tttggaccat atacgctatt ggttccagtg acatagcttt ttggtgtttt
     2281 tgctgttacc tctttggctg tcataccata aactgtcata ctcatacctg agattaaaac
     2341 tattgttgta atcaataact tggtagttaa cttcctaatt ttcttcatac ttaatattct
     2401 cctttataaa acattgttac ttcctattct ctaccattaa taaaattaat ttaggattta
     2461 aaaaggtgga ttgtcaacat gtatatttct ccattcatac attcgtgaaa tatttaaatt
     2521 tcatggaaaa tctaatagat attttctaga taatttgtat acaatacaac tcgcaaatca
     2581 gggtgtttgc tgttagtttg agaaaatgat gatgattaat acattcggaa tctttgacct
     2641 ataataaata ccattgtctt caatcattaa taatggtaag agtaaagaga attatcaata
     2701 atacagttaa aatcaacttt gtatacattt catacattct ctcccaacaa cattatactt
     2761 cttataattt gtatattcaa tacgttttct gggaaaatat agatatatct ataaaaatat
     2821 aaaaaaggag ttgaacttaa aatttgatta aaaaaaatca attttaagac aactcatttg
     2881 tattataaaa atgctatata ttattattaa gtaagtcaat attttagata tttatctatt
     2941 tcttattgac ttacgcttat agtctaactt atttgtttag ttgatatata acctagtttt
     3001 aagcattatt aatatttaaa taatcaagta tcctttttgt ttcttgtagt ttctgttctg
     3061 cctcatttaa gttttcttga attatagaaa tatatattat atctatcaac gtaagcattg
     3121 ctattcttga agaaatagct ccttctctaa gccttttttc tatatttggt actactagct
     3181 ttaaatcact aatcttacta agatgattgt cactatattt agttatacaa atgaccttac
     3241 atccttttaa ttttgcattt tctatagact ttattacttc ttttgattct ccagagtaag
     3301 atattgctat agctatatct tctttatcta catttgaaga taccataagt tgtgtatgac
     3361 tatctaaaga ggtaaatgcc tgtttattta tccttaaaag tttcatctgt aaatctagtg
     3421 caactaaagc cgaaccacct actccaaata tatatacatt tttagccttt attatctgtt
     3481 caactgcttc ttttattata tctttttcat tcaacaacct tgtttggttg atagtctcta
     3541 tgtttttgtt tgtaattttg tttataacac tatctatatt acaatcatag ttttcatcta
     3601 catatgtgta ttcattttct ttgcccatca aatctttagc tatctcaatt tttaaatccc
     3661 caaaacccga atatcccatt tttctagaaa acctaactat acttgctgta cttgtttttg
     3721 agtttgacgc caattctgat acagaaagct tacttatagc ctctgtatta ttaagtatgt
     3781 aatcagctat ttttttatct acttccgtta aatcttcata tatctgtttt aattttaaaa
     3841 tgcaactcat actctaccac ctctacctat ttactataat atttcttctt agcaagtaaa
     3901 catgctccta aatctggagt caacactggt ctcactatac caaatctcgt tttattcaaa
     3961 tattttttaa gtggctctaa tattaaatct cctgacttaa acactccacc tgagtaagat
     4021 acctttatct ttgtaccttg ttcaaaatga tgctctaatc ctattataag cctacttaat
     4081 tcataagctg catcatcaaa tattttacta gcagtattat caccttcaat aactgctttt
     4141 aaacatatca tagcaaactt tgctatttct attctatctc cttgtatttc atcatttaca
     4201 tattttatta tgtcataatc attctctata ttaagatgtc tttttattaa atcatataat
     4261 tttgtttttg aacaccttcc atcagactgc attgtgaaca tttttaaggt agccattcct
     4321 atataatacg ctgaaccttc atctcctaat acatagcccc atcctccaca tctgtgaaga
     4381 ttcccatctt tatctaaagc ctgtgctatt gaaccagtac ctgcaattat atttatacct
     4441 ttttccccat ttaaagaacc tgctaatgct atttctacat cattacctag agtataattt
     4501 atatgactat atgcattttt tgttgctact tctaatgcat acaatacttc ttttattttt
     4561 ccatatccag caagtccaac aaatgcatac gttatttctt ctacatttat cttagcatct
     4621 ttacaaactt cttccaaccc agttattaat aatttcgtta gattatcaaa tcctatttga
     4681 ttataatgac aagttccttt ggttatggtt cctactatat ttaattcttc atctactaat
     4741 gtaaatgttg ttttcgttcc tccaccatct acacctaaaa aatacatact atcctcctac
     4801 aatatcttta ataattcttt caatacttaa tatctaaaaa taaccttcca atgctgtttt
     4861 tttaatattg aaaggttatc agtactatta tttattttac aatttttcta ttgattttaa
     4921 agcttcactt atataacctt cttgtctatc taggatactc tttgcactat ccttatctaa
     4981 accacttttt atcataaata tagccaattt aacgctatta tctgcttctt ctaagaattt
     5041 ttctgcaacg tcatatttta catttgtagc tatcattatt attctttttg ctctttcaac
     5101 caacttttca tttgatgtct taacatctac catcaagttt gagtatactt ttcccatctt
     5161 aaccatagaa gctgttgtta acatattaca taccattttt tgagctgtac cacttttcat
     5221 tctagttgaa cccattatta cttctgcccc tactataatg tttattggta aatccgcttt
     5281 tttactcatt tcactattcg gattcatagt tattgatagt actgcacacc ccaattcctt
     5341 agcatactcc attgcaccta taacataagg agtccttcct gatgcagcta taccacatat
     5401 aacatcctta gatgtaactc ctctatctat tatatcttgc tttccaagtt ctttatcatc
     5461 ttcagcacct tctattgctt taaatattgc ttgatttcca ccagctatta tagcttgaac
     5521 aagctcaaaa tccactccgt aagttggagg acattctgtg gcatctagta ttcctatcct
     5581 cccactggta cctgctccta tatagaataa tctacctccc tgtttcattc tatctactat
     5641 tatattaaca gcttttgcta tactctcctt ttgttcttca acagcatagg ctacagtttt
     5701 atcttcgtta tttattaatt ctattatgcc taatgtatct tctttatcta tttgcaatgt
     5761 attcttattt ctaccttctg taactaaatt ttctaaagtt tttattaaca tatcatttcc
     5821 tcattttctt aatattaaat ttttaaacac attttaattc caactttcat aattatattt
     5881 ttataataaa ttaatatttt cataataaat tattttgttg agggtttaaa attaaacttt
     5941 tgccatggtt taatataatc taatataaat aattcttctt ttcttatata tcctactaca
     6001 ttcgatttac cactgttttt catatcagtc aaagctattt gtaactcccc tgaataatga
     6061 ccatattcac tactttcaat tactatatct cctcttttta ttattttagg agtattaaat
     6121 agttcaaatt tgtatccttt atatttaaca cgagattgag tagaccttat cagactgtca
     6181 gatatgtccc ctctattaaa atgtatttct tcaaagagta tcttcttctc aacttctcgt
     6241 atattttcct ctattgctac atcaaatgta accatatctt ttctcataga acctaatacc
     6301 tttagctcct catatgttgg ataacaattt gatattatta tatcatttat attattcata
     6361 gctattatat gctttacttg aatatctata ggtagatttc tgtgcatctc aattgttgga
     6421 agcccatcag ttacaggcca tggtccaaat gtggtttcat tttgacttgt tacaaaaact
     6481 gctgttctta atccatactt attaaatctt tctgtacatt ttataaagta ttctaaattt
     6541 aatccactat atctatgtgg ataaaagtta tgacatcctg tcaagttttc tttatttggt
     6601 tggtagtcca ttattgtatc tatataatta gtattattac tcatattgat ttctatttta
     6661 agattttgag gattaaaagt cattaatgcc tcttcagaac ctgtaaatcc catatcaagc
     6721 cttattccgt ctgccttcgt ctttttaaaa aaagtcaaat ctttgtagct tatttctaat
     6781 tcaccaaata ctctgggatt tacatcaagt attacctcaa aacctttaaa cttagcgtat
     6841 tcatttatac ttttaaattt tttaattata ttttctttag tatctttaac tgataataaa
     6901 catgaaaata tacgtgtaaa tccattttca gcagctttat ctatataatc atatatttcc
     6961 ttagatgctg ttttttctgg ataaattgat attcctaatc ttcccatctg tgtcatctcc
     7021 ttatatccta tatgtgttta aatttatttc gaattacacg aaactttttt aaaaatttgg
     7081 tgaaaaaaag tttcatatat atttataaaa aaagaaaagt ttaccttttc tttttttata
     7141 aacaatcaac aaaatatctt cgttacttat ttcataactg ttatactttg aaatttatac
     7201 taatctaata tagtttttaa tttccaatat agtttctaat taataagctt cttgctcttc
     7261 aactttttct tcatgaagca acctcttatc gtacatcttg acaaatggat agtaaattat
     7321 tattgatata gctatacata ataagttaag aactgaagct ctccaatctc caccagtggc
     7381 taaatatgcc cctactggtc ctggtaatgt ccaaggagca gtaactacta ctctggatac
     7441 taaattagca ctcatagcaa accatgaaac tgttgccatt actattggtg ccacaacaaa
     7501 tggtattatt aatattgggt ttaatactat tggtgctcca aatattattg gttcatttat
     7561 attgaatatt gctggtatta cagttgcttt tcctaaagac tttccataag ctgattttgc
     7621 tctaaatgca agaagtaaag ctaagcctat tgtagctcca gaacctccaa tccatataaa
     7681 ccattgaaaa aagggttcag cagcaatacc tggaactgct tgacccgcag catatgaagc
     7741 tgtattagca tctaataatt gtatccataa aggtcttgcc aatgaaccta ttattgatac
     7801 accatgtatt ccaaatgacc aaaaaaacgt tattaaaaat attagtataa ttactgatgg
     7861 taatgtgtca gtggctgaaa ctaatggagt tactaattta gctatgaagc catgccaatc
     7921 aaaccctagg aaataagtaa caatagccat taaaattatt attaatgcgg ctggtgtcag
     7981 agcttcaaaa gaccttgtga ctgatgctgg aactgcttct ggcatggtta tcttgaattt
     8041 agatttgtca gtaaatctat aaatctcaat acctattata gatgttacaa tagcaacaaa
     8101 cattgaacct ccaccaagat tagccattgg catttgccat ccttgaggaa ttgaattata
     8161 ccaagctaaa agttctggac tcttttcaac tatctttaat aaatcagggg taagaccctc
     8221 tattgcagta ggaactattg tcaaaagtaa tgtagctgta gctaataaac ctcctgtcaa
     8281 tccatctaac ttatatgact ttgccaaact ataacctatg ccaaatgtag aatatactga
     8341 tataataaaa aatgtcattc tatatggtag taatattgta actgcatttt cctttagata
     8401 ctgagttacc ccccatgaat ctggtaatgg aggaaatgct aatatcaaga aaaatgaccc
     8461 aactattata agtggtaatg aagctacgat tccatctctt acagctctta agtgacgttg
     8521 ttctgccaat ctggccatag aagttgataa tttagtgtct aaaaattctg taaatttatc
     8581 tagcatcttt tctctcctta attaatattt atttttatta tcattaaaat ttttataaaa
     8641 ttaactttct tctaatctaa caacactgca actactattt actcaacagt tcttgaactt
     8701 gttttaataa ctttggtcct cctaatggac tataagcttg tgcttgtatt aatgcacatg
     8761 gtacactaac ctcatcagca aaagctttta aatagtcaaa tctatgtctg acttgtggag
     8821 ctaccatagc aatatcccat ccatttttaa cttcattctc aaactcttga gtaccaacag
     8881 ccaaaacatc tatatctatt ccattttttg ctccttcttt ttgtaatgca ttaactgcta
     8941 tagcacttga catccctgat gaacaaacaa ataatactct catattgatt ccccctgttt
     9001 ttttatttct tttcaatcat tatagcatgt atttttgatt acaggaatac gtttgtatat
     9061 attttttcat attttacatt ttaaattgta attttatttc atacatttat atcttgcttt
     9121 aattgcttta tttatctaat cattcttttg tctaaaatta cacactactt aaaatactca
     9181 tatcttttct taaaaaagaa aaaagactat cttattatta acataaaata gtctcaatac
     9241 ttttttaaat atgtattcct ttatatatcc agaagtaacc tattattgca aatgataaag
     9301 caaataaaat atacagtcta aaaaatccca aagaaaaacc tctcttagtt tcaacaaagt
     9361 atcttgtata tgcaactgca aaagatgtaa ttaatgaatt tcctataaca actccaaatc
     9421 tattatctat tccaaatata gataataaca aaggaaaaac tacagtactt actagagatg
     9481 aaactattaa tattaatcca cttttaaaac ttatagttgg aatattataa tctgttcttt
     9541 gatttttctc catttgtttc ttagtatttt tattactttt tgtacttttt gcatttttat
     9601 aattattatt tttctttctt ttcttttttt tattattaga catgtcattt acctccaaaa
     9661 ggtttaggtg ttatattccc taaaataact ttatttttag cccctgttgc agatattaca
     9721 ttagaaaaag aattatttaa tgtttcattg cccaagatta caaatgccaa agcctccttt
     9781 gcatcagaag aatacccata ttcatcttga gttaatactt caacctctaa taataactct
     9841 tttatatatc ctattaaagt tagattatga gctcctccac ctccaattaa taaagtatct
     9901 acattaacct ttggaagtat gaagttttta taattataat atataacttt tgccgtaaac
     9961 atagttactg ttgctatcaa atcatattta tctacatgtt tatatttgtt aagcatgtta
    10021 tccaaataaa cttgtccaaa tacctctctt ccagtgcttt taggtgacgc ttgactaaga
    10081 tacttatgac tcatcaaatc ttttaacatt tcttcattaa ttttaccttt tgatgcaaag
    10141 tatccatttt tatcatactt tctattaaat aatctttgac atactccatc tattatcata
    10201 tttcctggtc ctgtatcaaa tgcaaataca tcatctatgt tacatgattt aggtattata
    10261 gtaatatttc ctattccacc aatattttga agtgctaaat ttttattgtt atcactatat
    10321 aataaaaatt cactatatgg tactaatgga gcaccttctc cacctgctgc tatatccatt
    10381 actctaaaat tagatattac ctttgtattt gtttcatatg ctataactgc tggttcacct
    10441 atttgaagcg ttgaagatac aaaattatta taacttcttg gtatatgaaa gattgtttga
    10501 ccatgagatg cgataaaatc caaatttgct atatgaaagt ttgctttatg acacactgat
    10561 tttacagctt tactaaataa atatccaagc ttaaaattta agctacagat taaatcaaca
    10621 ctcgattcct cttctataca gcactttctt atttcgtctt ttatatcttt aggaatttct
    10681 agagttataa attctatttg cttaacttta gtatttctac cattcccact aatttcagct
    10741 agtactgcat caactccatc taaggaagtt cctgacatta aacctacact atacatagtt
    10801 atcatccttt taagtttata gttattcctc cccaaatcaa ggtattaagt ttaataattt
    10861 tagaattaat cttatttcat ctttaattac gataatttac tcttacgaaa ttaatcgagt
    10921 taatcttata cacgtttttt accttagcta aatcttacat taatttattt tcagtgtcaa
    10981 tgtaatacca tgtaattttt tttcacaaac aaaacatatt ctctctccaa aaaccatatt
    11041 aaaatagtta ctacaaataa aaaacatacc aggtacctat ctgatatgtt tttcatatat
    11101 aataattatt attttataat atagatagaa catcatttaa ggcactaatt aaatcttcac
    11161 tattttcaag aactaaattt attcttattg tatatgtcga tttgtatatg atgatagatt
    11221 ttatagtcta ataacctaac tatctaggct taaataatcc gatatatcat aaaagtagat
    11281 attaaaaatt gtatatgaac caattttcga acatacctca atctcatgcc ctaaactatc
    11341 tatcattttc tttacaaaat acatccccat tccagtagac ttatacaatt gatttcttcc
    11401 atttttcccc gtaaatccct tgtcaaaaac cctagatata tcttcctcat ttatacctat
    11461 accaaaatct tttatagata ataccactcc attttctaac ttttttgaat taaattcaat
    11521 cttaccgact tcctttgaat actttatagc attattaata atttggtcta aaacatatga
    11581 catgcattta gaatctgtgt aaacatcatt ttctaattca ttcaaactaa cttctacatt
    11641 attttttata agcaaaaatg aattattttt tatagacatt tttaccaatt tttctaaatt
    11701 aaatttattt ataaatacat cttcaaacat agtgttgctt ctactactat acaaaataga
    11761 gtttactaaa aaatttatct ttgcaacctg attcttaata tccaaactat cttctatgct
    11821 agttaaccta tctgtaatta tacttaaaga agatattgga atttttattt catgaaccca
    11881 tttagctatg tactcatcca attcttttaa tgattgttta taagtctcta tgtcactatt
    11941 attttttatt tttaagtttt ctataatatc ttttattaga ttttcttcaa aagattgtcc
    12001 atcaactaaa taactgtcta tttcaccact attttctata caattatata aatttgtgta
    12061 actatttcta aaatttatat aatctataac aaaaaatacc attattataa acagcactaa
    12121 aaaatctaaa tatattaact ctgaatattt atttttaaaa atatctatag caaataagta
    12181 gatatttatt atactaacta caactaaagg tagagcaata tacttagacc taccttttaa
    12241 atattttact agcattgtat tatatatcct atccccttct tagtttttat aaaatcatca
    12301 aaacctagtt ctttaacttt acttctcaat ctattcatat ttacagtaag tgcattgtct
    12361 gtcacaaact catcattatc ccatagagtc atcataagac tttcccttga tacaacccta
    12421 ttcttatttt ttaataataa tgttaaaatt tttatttcgt ttttagtcaa ctctatactt
    12481 ttatccttat acttatgttt tataatccct ttccccacat caaacatcaa atcttcacaa
    12541 tacaaaatac tattagaatc actataatta tagactcttc ttattatacc atttattttg
    12601 gtaaccaata tttctataga aaatggtttg gtaatgtaat catctgctcc attatttata
    12661 cccattatta aatccatatc agaatctctt gatgataaaa atattaatgg tacatttgaa
    12721 atagctctta ttttttcgca ccagtaaaat ccattatata atggtaaatt tacatctaaa
    12781 agtactacat ctggtttttt atctataaat tcatctaata ctacctcaaa atcatcaatt
    12841 aattctactt taaatcccca tttttcaagt gctaacgcaa tctcttttga taaatcaata
    12901 tcgtcctcta ctaataaaat tctatacatt tgtatattct ccattatcct aatatttcac
    12961 tatgctatga attattaata tcataacata ttttatatga atttattacc caaattattt
    13021 agttaatcaa caaggagact cttattacaa gaatctcctt tctctttatt aatatatcaa
    13081 ctaattttta tacactacac tcttaaactt attaacagat actgctccat atattatgaa
    13141 tattatcaaa catatcacta aacttagtat atatatacca aataaatcgg tcatcaataa
    13201 atctcctgct aatctcaatg caaaataact agatatagtt gcacttaaca ttggcaaagt
    13261 ataaaaaata gctacttctt taagtatagc tctatttata tctttttctg tagtacctat
    13321 ttttaacaaa atcttatatt tctctttatc ttcatatgca tcactatata ttttcatata
    13381 tattatactt ccagctacaa ttgcaaagac taagaataaa aacacaagca ctacatatgc
    13441 aaacttcatc cattgactct caccttgagc tttatatcca ttttgaatat acccattttt
    13501 taaacttaaa ttcttttcaa tattgttgaa cattacttga ctattttctt cttgaaatac
    13561 ctttgcacca taaaaataca cttgttgacc cttatctctt agtttaaaat atgtatcatc
    13621 atttaccact ataatacttt gattaagtcc tgtccctagt actggaactt ttacttctcc
    13681 ccttgaaata ttatataact gaccatcaag ttttatcttg gcttcttttg tagctattga
    13741 tgctatcgtt tgagggcttt ttaactctat tacattattt ccactaacta atctttcacc
    13801 atactctttt aattctttat cattgccatt cactttaaga gtattacaaa aatcactata
    13861 tttcattata gtatctgttg tatcataagc ttcttttata tctgttttct ttaaaattgt
    13921 tagttctgga tttactgagt actttatctt attagtatct tttattatat tctctatttg
    13981 ttttttattc acttcatctt tatccattga aactatgcta atagtgtaca catttctttg
    14041 ttcttgggca ttcttatgta tatgcttcat agcaactgaa gcaccaagag ttgatattgt
    14101 tgtagttata attatagcta ttatagcata agttttatag tttttattta atcggtagtt
    14161 tatattgttt attgcaatta tattatttcc attaaataat atttttttat ttttgattat
    14221 aatatcaaat ataacaggca taactgattt gaataagcca taagtaccta ctattattat
    14281 acctataata ggtagtacac tagttatatc tccagttctt atattggtag aaagatagta
    14341 gccatatcct attactataa ttgatactat agctaatata tatataccta ttcctacctt
    14401 tggtattttt tcttgttttt tagatatatt gattagattt attacactac ttctacatat
    14461 tgttataaaa ccttttacag ccattattat aaatatagaa ataaagatta aagtagcata
    14521 tagtatagct tctatactaa gtccattaga tacctttata ttaaatccag ataactttat
    14581 tatcactact tgaaaaaatc tagatgttat tatccctata attattcctg ttatacaaga
    14641 gcttattcct ataagcatat tttctacaaa atatattttt cctatagtat ataaatctaa
    14701 tcccataaaa gacaatattc caatttcttt agacctattc ttaaaaaata tatttgttgc
    14761 ataccatata aagaaaaata aaaatactga tagtatcatt attacagcta ttaacactga
    14821 tagtgtaaat tttttatttt cttcttgtaa cacatttata gcatcaccat atcttaataa
    14881 ttcaaaatta gttagcacaa ctactgaaaa aaccatagat ataaaaaatg ctagataagt
    14941 tcttatgtta ctcttaaaat tattataagc aattccaagt atactcactt acatctcacc
    15001 tcccatagag gctagcatat ccataatttt tccataaaat tcttttctac tatttccttt
    15061 gtttaattta cactttatat ttccatcatt tatcatatat acttcatttg aataacttgc
    15121 tgttagaggg tcatgtgtaa tcattataat agtagcgtta ttttctctgt ttattttttc
    15181 taatgattct aaaagttcat atgctgattt agagtctaag gcgccagttg gctcatcagc
    15241 aaatatgact gttggatttg taattaatga cctagctgct gcgcctcttt gtttttgtcc
    15301 tcctgataac tgatatggat atttatctaa atgattttct aatccaaatt ttttagctat
    15361 agaaaatacc ttagcttcta tctccttact tttctttttt cctaatgcta gaggaagtgc
    15421 tatattgtcc attagtttca tattgtctaa tagattatag tcttgaaata taaaccctat
    15481 tttttctctt cttaattttg atagttcatt attttttact tttgatatat taattccatc
    15541 tagtattatt tctcctgaag tttgtttgtc aagtgtagac attaaattta gcaatgttgt
    15601 ctttcctgca ccagatggac ccattattgc tataaaatca ccttcattga ctgtaagaga
    15661 tatatttttt aacacatttg ttttaaatcc ttttattcca taatcttttg atatattttt
    15721 tatttttaat atttctttca taataatagc ctccagttgc taatttttta atatatattt
    15781 attttaaagg ttttatataa ataaatcctt aagtaaatat tacattttaa ctgttagcat
    15841 tacacttttg taacatatgg aagactatta taattatagt ggaaatatct taaaataaaa
    15901 acttgcttca cttctttgta taaacaaaga aaagaagctc ctaagaagcc tctaatctat
    15961 acaataattc ttctctataa aaaaccaaaa tacacattgg ttgtggcaaa tacagttgct
    16021 ataaacagtg atattactgc tatatattaa aacaattatt actgaagata attcaataag
    16081 actatcaagc cataagctaa gcatccaaaa agacttaata ccattaatgt ataattatac
    16141 ccaatattaa tgctcaaaaa accagcaaca gttggaataa tcagagatga acctcccata
    16201 aatatactta aagctattaa ttggctacta cttctaagtt ttttaggaga aacttcaaat
    16261 attaatcttt ttgaagtaat taaaattaaa ggagtagaaa acacttgaaa aacacacagt
    16321 ataataatga tatttgaatc atttgttaaa gcaaataata gaaactggac tgttatcata
    16381 atagcactta actttaataa gtttaaactg ctatattttt ttaataaata agaaccagcc
    16441 agatatgttg gtatctctaa taatgactgt aaagaccact ttaaagatat ctgagaattt
    16501 gtagcattta aagctatcat tttatctatt acagtacagt tatttgctac taccattgag
    16561 tataataaga ataaaataca tactagtaag atatattttt tatcttttaa taattcaata
    16621 atatctttat tagttacatt tattttctta ttaatctttt caatatcagg taataatagt
    16681 agatttataa taagtaatac tacaattaaa ccaatcgcta cagataatcc aaaatatgag
    16741 aatctaagaa tcaagtaaga tgctaccata cttccaatag cccatccaat cgacccaaaa
    16801 gtcttaataa atgacaaata attattaata ttatctccac aacctattat ccatgtatca
    16861 tataaactac agcaagtatt taataatcct cctgataaag caacaagtaa taagtatatt
    16921 ataaaagttg cagattcagt agaaaacata ataactgatg caattccata aactgataaa
    16981 ctaattaaaa caattttttt cattgtttga tacttatcag ctaatactcc aaataataat
    17041 tgaaataata tattggttat tgcatagctc gataatataa ttcctctttc aaacgctgag
    17101 taacctaatt ttgataagaa aggtgtgaat tttgttgaag caaaggcaaa aataatatat
    17161 gaaaaaaaat agaatatgat aaatttaaat ttaatcgctt tattaatcat aatcaatcaa
    17221 aattgataac tttaatacca ctatttcctt cctgatttaa gtttgaaaga tgatatttta
    17281 ccgtattagg attctctaca ataactaaag gaatatataa agtattttca agtaacttat
    17341 tatcaatttc tactaacttt tcccccatat taacttcttt accttgttta acttctaatt
    17401 taaagttttc tttttttgtt gttgcactgt ccaatccaat atgaataaga atttgaacat
    17461 cattttgcaa tttaattcct atagcatggt tagtttgagc aataaaactt attactcctt
    17521 tagtcggtga tttaagaata ttgtcctttg ctatcatagc aaatccatct cccattacct
    17581 tagttgaaaa tactttatca ggaatttctc caagattaat tatttttcca tcaatgtatg
    17641 aattaattgt aattgcttct ttttttcttt taaagaacat tatagacact cctctacata
    17701 attaacttta ttctaattat cattacaaat aacgatatta agcatactgc aattgaggaa
    17761 aaaagagcct tatagctctt tccccattgt agttctttaa tactgcttat gatttgtcta
    17821 attccaacaa atttataaat ataggtaaac atgataacaa tagttattgt gtaaatttgg
    17881 aatatctgcc ctaataagac taaatagaaa gcaatcatgt tttcttttat ctctttattc
    17941 ttttttatat tgtaaataac atatgcactg tatactgcac taaagttaga gattaaattt
    18001 aaacttgcat taacatcatt gtttaaaagt tgtgaaaatg aaatgtttgt aaataatgat
    18061 gtcagttcta aatacactat atataaaaca ggtaatatta gaaaacaaac cataaccact
    18121 gaaagaaata tattttcatc ttttaatact gctgtgatat tttttatttt atttgttatc
    18181 atgtttttgt tctccttatt taaaagttgg ccagaaatcc ttattagcca caatcatttc
    18241 atctaagatt tcttttgcta cttttgcact tggaacagtt ttactcatag taaacgactg
    18301 ccataatttt tgataacttt tttctatcca tgcttctaca gctaattttt cagaagcaac
    18361 ttgttgttcc attaaacctt tttggaaagt tggaatttta cctatagaca taggttcaac
    18421 catatctttt ccaacaaggc aaggaatttc aaccattgca tcatctgcaa aattttcaat
    18481 agctccatta ttaggaacaa tcagtaaaaa tctttcctga gtattaaatg ctaatgcaca
    18541 agctaattca acaataaatt gagaatgttc acttgcatca aaccatgtat ctcttgctgt
    18601 gccatttttt ataattcttt cacattcttc gaatgtatcc ttttctcttc tatccataat
    18661 ttcatttgct cttgtataat ttggattagc atgttcaaca gtttcatcag ccattaaata
    18721 atattttaaa taagtattag gtaatgtagt tggttctatt tctaataagt cttttgctgc
    18781 tctcatagtt tctaaccaac tagattcttt gtgttgacct tgaggtgtaa attcctcata
    18841 tcctttttta gcacaatatt cttgtaattt aggcattaaa tcatttcctt ctttgtcttt
    18901 aatacttgtc caccacccga aatgattcaa tccataatat ctaacatcaa agtctttacg
    18961 agaattcaaa cctaaaactc tagcaaatat ttcctctaaa catactggca tatcacaaat
    19021 attaataatt cttgaatttg gtcttaatac acgacatgct tctgcaacta tagctgctgg
    19081 atttgaataa ttaagcatcc aagcatttgg agaatatttt tccatataat caatcatttc
    19141 gattattgga cctattgttc ttaatccata ggcaattcca cctgccccac aagtttcttg
    19201 accaacaacc ccatatttta gtggaatttt ttcatcttgt tcacgtaaag catataaacc
    19261 aacacgaatc tgtgccatta caaaatcaat atcagtaaat gcttcttctg gatttgttgt
    19321 ataactaaat ttaatttctg gatttttctc tctaacaatt attgcacatg cttcagcaac
    19381 ttttctttgt ctctcatcat tattatcata taattttatt gattcaatag gaaatttagc
    19441 aacactttca attaacattg ttacaattcc agcagtataa gtactacccc caccagcaat
    19501 tagtatcctc tgtctttttc tttccatctt tccttctcct ttataataaa ttttcaaatt
    19561 cttctcttac acttggtact gaaagcccaa taataatctg aatagctttt ccattatgca
    19621 ctaaaccatg tgcacctact gccttaaaat cttcttcttt agaaataatt gagtcatctt
    19681 tcaaggttaa tctcaacctc gtagcacagt ttgtaacatc tacaacattt tctgctccac
    19741 caactaattt tatgtattca gatgcttgtt gagaggcttt actcaattta tttccttttt
    19801 catctttagt ttctaaatat tcatttttag tatatagttt tgtttcttca ctatcttcct
    19861 ctctaccagg agttttcata tcaaacttct taattaagaa agtaaataca aagaaccaga
    19921 taaatgaaaa tataatccct acaacaactt gaacaacata taccttccag tgatacattc
    19981 caagaggaag ccaatttaat acaacccagt taataattcc tccaccaaaa tcaccaacaa
    20041 ctcctagggc aaacgaaatc gtttgcaagc atgcatctaa aaatgcatgt actaggaata
    20101 aaagtggtgc tacaaataaa aatgtaaatt ctaacggctc agtaattcca gttaatactg
    20161 cagtaagagt agcaggaatc attaaaccta atactttttt ctttttttct ggctttgcag
    20221 ttacatagaa agcaccaaat acacctaatg aaccaaacac cttcgataat ccagataatg
    20281 aaaatccgat tggatacatt tcctttagag tcttagctga agtttgaaag tcctgaatat
    20341 gagtagccca atatactgat gtaccacctg gaacaacaac actatcataa caaattggtg
    20401 catatacaaa atgatgtaat ccagttggta ttaaaatctt ttgtaagaat gcataacacc
    20461 aaactcctat atttccacta tttataaaaa acgtttgcaa atgtctaata acattttgaa
    20521 tatgaggcca aataaaacaa gttaataatg caacgggtat cataacaaag aaacaaattg
    20581 caactacaag tgatgagcct ctaaataatc caataaaatc tggtagttct ttatcataaa
    20641 atctattatg aatataaatg acaatacctg atattaacaa agcaccaacc ataccagtat
    20701 ctaatgtttt aataccagca atgcttacaa gtccacttcc agcacttatt tcagctacga
    20761 aatcaactcc aaagcttttt ccccaaaaac ttaacataga acctacaaaa taattaaaag
    20821 tcaaatacgt tgctaaagcc tccatacaag ctcttgcttg ttgctttttt tcaagtgcta
    20881 ttggtaaaga aatagcaaat aataatggta attgattaaa taccatccaa gctccactag
    20941 atattacacc ccaaaattgg taccaaatat tttctggtgt agcaagtgaa cccataattg
    21001 actgattttg aaatacaatt gagaatccaa ccattatccc agcaaaagca aataatagtg
    21061 ttggtgtaaa cattgcccct ccaaaacgct gtattttctt catcatataa attactcccc
    21121 tcctcatatt tttagtttat ctttggcctt agatatctat tattttaaca ggttaataaa
    21181 tatatacaat ctttagggta cattcggagc ttattcacag tcaaataagt ttttgcacat
    21241 ttttatgttc aaattctcat aaaaaaaaga atgactacaa tttgcaatca atcccttctg
    21301 taatcattct atgcctttta taaatactat atttaataaa taataactca atcaatataa
    21361 aatataacat tgtcaattta taactagggt gcatttgtaa aaatgagata ttagtcgctg
    21421 aaatataaat tgattcatca cttaaatcag tcaatgtatt atttttaaat tctgtaattg
    21481 aaattatttt agttcctatt aattttaatc ttctagcaat attaagtgta ttttcattct
    21541 caccacttag ggatataata atcataacat catttttact gacaatattg taaaaagaat
    21601 catcaaatgt tcttccctca aaatcatata ttatttcttg acatgataag aacattctct
    21661 ttatctgttt tgcaaccaca ttttgaatat cacctgaacc ataagcgtat attgttcttg
    21721 attcataaag aagtttacac aatgcatcat aatctttatt tttttgatct tcaacatact
    21781 taattattga attacatgca atatcaaatg actgattttc tatgacatga accttttctg
    21841 attcccagct taataacact ttaaactcac tgaaaccttt taaatctaat tttttaacaa
    21901 atcttgtaat agttgcacga gacacattac atttcatagc aagttcattt attgacatct
    21961 tactacaatg ttttttattt gcagttatat atttccatat taataaatct gtatcattca
    22021 atttattata atttaaaaaa atcatttctt ctaatctcat aaactattac cccctttaat
    22081 taaaaatcgt cataaatatt ataccaatat tcaacattaa attacagcgt attcttatat
    22141 ttactattta gcccataaaa ttgactataa aaaatactgc tataattgat gtttaaaaaa
    22201 atgtagtttt aattttattc ttttatctaa taattaatat tttcaagttc tagtgctttt
    22261 cttatacaat tatcatcttt aaaatcagca gtccttttac aatcttttac tttaaattct
    22321 ggaatagttt taaattcctc atcacatatt ccattcttat ttaaatacat acaacttgcc
    22381 attctaacta ttagaccact attttttaaa ttaaacaata cagggtctat acaccctcca
    22441 tctccgccag tagtttcacc aactaaagtc gcaaatttag tttctttaca aaacattgca
    22501 aatgactctg atgatgaata tatttttcta tcaacaagta agtatatatt acccttaaat
    22561 tttgaatcat ctgtagattt tatagtatag gaagtatctt caaaatcatt aaaatctttt
    22621 ataatttctt taggtgcatt ttcctttaca cttgttggta aattttctac aggatttaat
    22681 tttatatttc ttgcatttgt atattgtttt actatttcat tattatttct ataaattcta
    22741 taaccattta ttttgacatc attttttatg agtttagata cgattccctg ccaataacta
    22801 tcacttccac ctaaattacc tcttatatca ataattaaag ctttatgttt ttctaaagtg
    22861 tcaatataat ctccaattat ttttaaatca ttatttatag agccattctt tgatgacatt
    22921 gatggtaaat acatatatcc aatctgaccc ttaataacat cctttaaaat taattctttt
    22981 tttacaaaaa tatcatctgg catttttatt ttaagattca tagagtcata tctatctaca
    23041 acttttttat cattaagaaa atcataccaa tcattattag agtatgattt cttaaaaagt
    23101 tcatatcttt tcttattatc tattacttct gtgtgtttat tatttaaatc agataatata
    23161 gatgacagtt cttctataaa ttcttcatca ttttcagtgt tttttattcg ttttgtatat
    23221 tcatccttat ttgctaacca atcaatatta ttaagcctct tatttacacc tagatatgga
    23281 tatcctgtct ttacaacatt atacacataa ttaaagtcat ccaccttttc ctcttcagac
    23341 aactgattct tttgagaata agtagaatta cctaataaga ttataaatgc taaaataatt
    23401 actaaaaata atactttctt tttcatgtaa ttctcatcca ctttcagatt ttatatttta
    23461 atacattgta tcattatttt attatcacct aaacagttaa aagaaattct tgaagatata
    23521 ttccactatt attctcttta aaaattttta tttgtatttt caaaattatt tcatgaaagt
    23581 tataaaataa aaattatatt caaatatcta ttttactgct atatcctgaa agtaaattct
    23641 tatcagtgat atactaaaaa agatatgcta aaaaatttat ccattataat gacttaaatt
    23701 tagatacttt actgattttt atttattagt tgagaaagat attgataatt aaaaatattg
    23761 ataactatag aaatgattat tttttataga aattttaagg atataaacta attatactta
    23821 agtctatatc cttaatttat catgattatg agaattttta caaatactta tctttgtccg
    23881 ttttagaata aaaacaagaa catatcctgt tattttattc aaacatccaa gtttaattac
    23941 tacacaatta cttattagaa attgtatcta cttcatatat atatctgtta agtatagctt
    24001 ctagcatttc ttgtcttcca gacttattct ttataactgg catttgcagt gcataagatt
    24061 ctaactcttt aaatccaacc ttaccttcta ctatatcctt acctatacct tcactaaaac
    24121 ttgcatatct ttctttagta aagttctcaa atacctcatc ttctaataat ttatgagcta
    24181 ttaataatcc ctttgcaaat gtatccattc ctgcaatata agctaggaat aagtcttcta
    24241 cctcaaaaga tgctcttctt actttagagt caaagttaaa tcctcctggt gctattccac
    24301 cttgctttaa cacttcatac atagcaagtg ttgcatcata tatatttgtt gggaattggt
    24361 ctgtatccca acctaataat aggtctcctt gatttgcatc tatactacct aaaacattgt
    24421 ttattcttgc tatatttaat tcatgttgga aagtatgtcc tgcaagtgtt gcatggtttg
    24481 cttctatatt cattttgaag tatttatcca gattatactt tcttaaaaat cctaaaacag
    24541 tagcagtatc aaaatcgtat tgatgtttag taggctcttt tggcttaggc tctattaaga
    24601 attgtccagt gaatcctatt tccttagcat aatctacagc catttgtaag aatcttgcaa
    24661 agttatccat ttcaagttct gtattagtgt taagtaaagt ttcataacct tctcttccac
    24721 cccagaatac aaaattttct cctcctaagt acttagttat ttccatagct tttttaacct
    24781 gagctgctgc atatgcatat acatctgcat tacatgttgt agatgcacca tgaacaaatc
    24841 ttggatttcc aaacatattg gcagttcccc ataataacct tatacctgtc ttttccatat
    24901 taacttttat atactcaact atttcatcta atatcttatt tgtctcttct aaatctcttc
    24961 cttctggtgc tatatctctg tcatgaaaac agaaatactc aattcccatc ttagacataa
    25021 attcaaatcc tgcatcaact ttaatctttg ctatctcaat tgggtctgtt atattattcc
    25081 aaggtctctc tactgtccct actccaaatg ggtcattacc ctctccacat aaagtatgcc
    25141 aatagctcat tgcaaatctt aagtgttctt tcatagtctt attacctacc acttgttcag
    25201 gattgtagta tctaaatgct aattcattgt cagattttac tccttcaaat tttatctgtc
    25261 ctattccttt aaatatttca ctcatttcat gtcctcctaa tattttacac atataatata
    25321 tgtagtatgt tttcttaatt taaaactatt ttctatcttt atttttcttt ttaagctaat
    25381 ttaattattt atttttgcat taactacttc atctaataac ttaaagtcat ctttcaaaga
    25441 tttgtatatt cttttataca atctatgata tatattgtat gtatctacat tttcacttat
    25501 tggttctaat ttagttgaag tttttattat agcatcacaa gctttttgta tattttcata
    25561 tattccagct cctactaatg caagaattgc aactccaagt gctggtcctt cacttgaatt
    25621 tatagtatat acatcttgat taaacatatc tgcttgcatc tgtctccata ctttactttt
    25681 tccacctcca cctgaggctc ttacttcatt aacgtctata ttaagattct ctattatatc
    25741 catacagtct ttaagactat atgaaacacc ttccattatt gaccttgcga tttctttttt
    25801 agaatggatt ggagataatc caaagaacac acctcttgca taagggtcaa gatgaggggt
    25861 tctttctccc atcatatatg gtaagtaaag caatccatta catcctgttg gtacttgact
    25921 tacttgatta tttataatct catatacatc ttcacctaaa gaatttgaaa cttccacttc
    25981 actttgacag aaattatctt taaaccattt taatgacaag ccagctcctt gagtaactcc
    26041 cataacatgc catgtatttg gtattgcatg acagaaagta tgaactctac cttctttatc
    26101 aatttttggc tcttttgtat aagcaaatac tacacctgat gtacctatag tagatgaaac
    26161 tactccatct ttaactattc catttcctat agctcctgct gcttggtctc cagctcctcc
    26221 taccactatt gttccttctt taagccctgt caaacttgct acatctctag tcacataacc
    26281 tgtaatctct tgtgattcat aaactttacc taaaagattt ttatctatat ttaacttttc
    26341 tagtacttca tcgctccaat ctctttttct tatatcgagc aactgcatac cacttgcatc
    26401 tgatacctct gtagcatatt cacctgtcaa tttgtatctt atataatctt ttggaagaag
    26461 tattttatta gtatttttat atatatctgg ttcattgttt ctaacccata atatctttga
    26521 tgctgtaaat cctgtaagtg ctggattagc agttatttca atcagtcttt cttcacctac
    26581 taaatcagtt atctcctcac actcttttac tgttcgttgg tcacaccata ttatagagtt
    26641 tctcaatgtt ttaccttcat tgtccagcat aactagacca tgcatctgtc ctgataatcc
    26701 aattcctttt atacattcac tgcttatatt tgaactttga actacttctc ttatagattc
    26761 gactacagct ttccaccaat catttgcatc ctgctctgcc caacctgatt tctcttgtat
    26821 aagaggatat tcatatgtac aattttttat agtatttcct agtttatcaa ataaaactgt
    26881 tttcgtacct gatgtaccaa tatccacacc taatacatac tccaacttca tccctccttg
    26941 aatgccactt cataactatc gttttcctat cctaattttt ttatatttta ggtatatttt
    27001 acttgcttta tttttgttag tatattaaac taacttatga actaataata aaatatttaa
    27061 ttatctttgt caatgcaaaa taacctttta tttttgtgta aaaagtatta caatattatt
    27121 ttaaggagga atatcaaatt ggttactgat aaatacacta ttcgagaaat gaatgaaaga
    27181 ttggtacttg aacaaattat aaaaaatggt cctatttcta gggcaagtat agcttctact
    27241 ataggattga ataaagcaac tatatccgct attaccaaaa aacttattga tgaatctcta
    27301 gttcatgaaa tcggaattgg aaatagtact catagtggcg gaagaaaacc catattatta
    27361 gtatttaata aatgtgcagg gatttcttta agtatggaca ttggatacga ctatatattt
    27421 tcttctctat cttacttaga tggaactatt ataaattcta agaaattaac agatatacaa
    27481 gtaagtaaag acaatgttat acaattgata gatgaaatta taaatagtta taacatatcc
    27541 aaaatagaca ctccttataa ggtaattggt cttactttgg ctatacatgg gattacttgt
    27601 gaaaataaag tattgtttac tccatattac aatcttaatg agattgactt atatagcatc
    27661 cttagcaaaa agtatgactt tcctattcac atagaaaatg aagcaaactt aacagcttta
    27721 gctgagaata ctttttcaac tgtacataac tcactactta gtctcagtat tcacagtgga
    27781 tttggttcag gcattataat aaataataaa ttatatagtg gcagaaatgg tatgagtgga
    27841 gaaattggtc acactatcat catgcctaat ggtaaactat gtccttgtgg aaatcgtggc
    27901 tgtttagaac agtattgttc agaaaaaaag gtatttgaac agctttcttc tttagaaaat
    27961 attcctaaaa tagattctga tatagtaaag caactatatt atgaagataa ccagaatgcc
    28021 aaaaaagtta ttcatgagtt ttgtagttac ttaacaatag ctataaataa tgctattaca
    28081 acttatgctc ctgaaataat atatttaaat agccaaataa ttagtgatat ccctgagata
    28141 ttacagatta caaaagatat gttagttagc tcttttaaca aaggaataaa tatagaaatt
    28201 tcatcattag ggtctgaggc atctctatat ggtggctctg ccgtaaatat caagagcttt
    28261 ttgaatatac aaaatttaac tttaattaat gaaatataag ggttatgact atatacagcc
    28321 ataacccttt aaattataca ttctaaaatt agatttatta tatagtttat gcaagattag
    28381 ctatattttt taaaaattgt atatcaatta agtattaaca taagtatttt gttttaaata
    28441 ttaatataat cttcctactc atcatcagat aataaacttg cattttgttc aaccatagaa
    28501 atattatctt tacctatttt ctctaatttt gctgccaatt cctttgtatc taaaaaagct
    28561 gattcaataa ccatacctaa atttagccct gaaataattt taattcgact gtcattattg
    28621 gctactaata aagcagcatt gcatggagtt ccacctagta aatctgccat tataattatt
    28681 tctgaacttt ccttgtacac ttctaaagct ttttttaatt tttctattgt tccttctatt
    28741 ccatcatctt gtttcatatt tacagtagga taatctatag aatcccctat tatcattttt
    28801 gcagaattta agacttcttc agccatattg ccatgactca tcaaaataat actattcaat
    28861 ttaatacacc tctttaaatc tataagatgt ctacaagata agttttctta tcatcaggcg
    28921 tcattatagt tgtaatttca attccttcat ctaaaagtct tttaaatgct tctttctctt
    28981 cttcagtaac actgatatta ggtcttagaa ctgttgtatc ttttctactt gacatatttc
    29041 ctacatttat attatgtatt ttaaatcctg cctctacaaa tggaataagg tccacaggag
    29101 acttaacaat taataataca ttttgtcctt catactttcc tgcagtaata ttttttatag
    29161 cagtctcttc tgtaattata gatgacgcta tacctgcagg tgtagccatt cttaaaactt
    29221 gtttttgaac agaattgtta gctactgcat catttactac cattaatctt gtaacaccca
    29281 atttattact ccacatagtt gcaacttgac catggattaa acggtcatct atacgaatat
    29341 ttttaatacc tttcatttcc atatctaata tcatcctttc cttattttaa aatacctaat
    29401 gcatataaaa ttattgaaac aaccattact cctaaaatta agtatatagg ttttatattc
    29461 tttttaagag cccaataaac aatcacaact aaaattgcag gaactaaccc aggcataatt
    29521 tggtctaata tttcttgacc tttcattgta aaatcacctt gttgaaagtt caaagcaaca
    29581 ttagctttta caactgaagg tattaatgca cctacaacca ttaaacctaa aatagatgta
    29641 ctctcagtta aaatctttaa acttttttca attgaagata ttagcttagt acctgatgta
    29701 taaccagcat taaatagtgg tatacttaat aatttcaatg ctacacttac tacaatccac
    29761 agtaagattc caaatggact tccatctatc gccatattag ctgctatagc tccaaaaatt
    29821 gtccacggaa taacgacaaa taaactatct cctaaacctg ctactggtcc catcaagcct
    29881 gttttaattg aagcaacagc ctcttcagtc tcttcacttg ataactcctc cattgctaca
    29941 ttaataccca aaatataagg ggcaacccaa ggattagtgt taaaaaattg taaatgattt
    30001 aatacagcac tttgtaactt ttctggatta tctttgtata ctacttttaa aaaaggtagt
    30061 actgcataac agtatgccaa ggcctgcatc ttctcataat ttgaagaaac agaactagta
    30121 aaaaaccagc gccataagac actttttttc accttttcat ttgatttatt taatgatacc
    30181 ttatcactca tcttcgtcca tacctcctac tacagtatat tgttgttctt gattttccaa
    30241 attctttttg taaattataa gtgcaattgc tccaccaatt aaagctacac ctaataatgg
    30301 tactttcata tatactgcta aaacaaaacc aattactaag taactaaaat actccttagc
    30361 aggaagatat ctaagtaata atcctatccc tactgctgga agtagtcctc cagctactgt
    30421 taaaccacct gttaaccatt caggtgtata tttcaaaatg gtattaatta aagaaggtcc
    30481 aaatattaca gatagagcaa ctggaatacc tgttgtcgct gctgttaata gtactcctaa
    30541 catttgcata taggttatat ttttataatt tcctttttta gctccatctt ctgctttatg
    30601 aactagccat ataccaatag tatttctaag aacatctaat tgtaccatca ataaagcaac
    30661 tggaatacct atagagattc ctacagatgc ttcttgctta gtcgtaatag ttataaatgt
    30721 ggctactata cttgctgttt gataatcagg tattgatgct ccaccaaagt tactaatacc
    30781 taatgataat agctgtaatg tacctccaat atataaccct gtatttatat ctcctaagat
    30841 aagaccagta ataaaacctg ctgttactgg ttgatatgtt ccaaacattg ttgaattttt
    30901 ttcatagttt ataaaaaatc catatagtac gattaataat aattgccata attgtggctc
    30961 cattatgatt tcacctctcc caaaatattt tttagttctt taaaattata actggaatct
    31021 tgctttagga aaataggaat ctcttccagt gggcattcaa cttcataagt tttcccacct
    31081 tcataactaa ctccactaaa tatattttcc catgtatgtc cttttggtaa atatactttt
    31141 ctacttctat ctttataatt cattattgga gctaccaaaa tttcatctcc aagcatatat
    31201 gtattatcca cctcccaagt tttatcatct tcaggaaatt cataaaataa agttctcata
    31261 actggtgttc catcttcatg cgcttctttc atcagttttt ttaaataaat ttttaagctc
    31321 tctctaattt ttatgaattt tgtaagaatt acttcaactt cttcaccata agaccatatt
    31381 tcattaggag ctccagttac cattgagcct ccacctttat ttgataatgg tttactatga
    31441 ggcaatctat ctccatgcat tctcaaaatt ggtgaaaaag tagcatattg gaaccatctt
    31501 accataagtt ctctaaattc tgggtcttta ggatttcctc catggaaccc tccaatatca
    31561 gtagtccacc atggtatacc tgccagtccc atatttaacc ctgtattgac ttgattatta
    31621 aaagcctcaa aactagaatc aatgtcacca gaccatacta aagctccata cttttgagct
    31681 cctgcccaag ctcctcttac aagagttaca acagactcta tgtcattatg taaaccatca
    31741 taaatcattt ttaaataatc aataggataa atattaccac aacttaaaac atttcctttt
    31801 ttatatctat agttatcaaa atcatacaca gaatagccag gttcagctac atctaaccaa
    31861 taatagtcaa tcccaagttg tttataattt ttatctattc tatcccaaac atattctctt
    31921 gcattttcat ttgtcatatc tacaaagact gtatttcctt gaatttgcat tgtcattcta
    31981 acaccacgat taacattgac taaatagcca ttttctaaat aatcattgta gttttctgca
    32041 tcactttgaa ctgtaggcca tacagatact attggttcta ctgaatactc ttctttcaat
    32101 tttttaacta attctttagg atttttccaa taatctaagt caaatttata ttcaccttgc
    32161 ttaggccaat gaaaatagtc aattgcaatt gatgaaagct taattcctct tttagaatat
    32221 tccttcacaa catctaaaac ttcttctgaa gttctatatc ttagtttact ttgccatagt
    32281 cctaaaaggt tttcaggcat cataggaaca gttccagtaa cttgagaata atttttattt
    32341 aactcttttg ggctttctcc acaagtaatc caataatcta taaaatttgt agaaaacatc
    32401 ttccattctg tcatattttt agcaaaagat acttttccaa ttcccggatt attccataaa
    32461 agtccataac ctagactaga aatataaaaa ggaactgata tttgagaatt tctttgggct
    32521 aactccaaaa cagtattctt taaatctaaa aatttatgtt gatattgtcc cattccaaaa
    32581 atcttttcag aaggctcaga ttcaaatcta gtagttactt caaattctcc actatagctt
    32641 ggtcgatatt ctcttgattt taactttaga gtagagttaa agtctttggt aatctcaatt
    32701 gtccctacat cttctccacc atcatcatgt tttacagctc ttaatcgaat atattctttc
    32761 aataatattt catttttatc attataaaaa gtaattctat ctctatggtc taagacagct
    32821 tttatctttc catttttaat gattgcactt ccatcttcat ttttattaat tgtaatatca
    32881 tctttattta acttaggttt ttcgttaaga gcatagtttt catcaacaaa attttggtct
    32941 acaaacgacc taatacgtaa agagtttttt ccccatgctt ctattcgcaa tagttcattg
    33001 tcaaatcttt tttcaatata gtcttcagat atttttatca cggctacctc ctaaatcaat
    33061 ggtataatat tctatctttg tgtagcttaa gttaaataat gtcgtttagt atcgttattt
    33121 ataaattagg ttttatatat aattcatttg tacattaaac taacttacca ataatatact
    33181 ttatttgact ttgtttgtca atcgctttcc ggattttagt aaaattttct aatatattgc
    33241 tggaaagtcg ttcttaatat cttttattta tacttttaat atctgtgatg atacctatat
    33301 acatctagtt tttttaaaac acatacagga ataaagtttg cctattatgt agataatatc
    33361 ggacttgaaa atatgtatag tga
//
LOCUS       DAEMHW010000020        33341 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEMHW010000020 DAEMHW010000000
VERSION     DAEMHW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.020"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(139..1176)
                     /locus_tag="KPK67_000821"
     CDS             complement(139..1176)
                     /locus_tag="KPK67_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG2457822.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(1195..1980)
                     /locus_tag="KPK67_000822"
     CDS             complement(1195..1980)
                     /locus_tag="KPK67_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG2457823.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(2013..3146)
                     /locus_tag="KPK67_000823"
     CDS             complement(2013..3146)
                     /locus_tag="KPK67_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG2457824.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(3194..4321)
                     /gene="hadC"
                     /locus_tag="KPK67_000824"
     CDS             complement(3194..4321)
                     /gene="hadC"
                     /locus_tag="KPK67_000824"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBG2457825.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(4321..5547)
                     /gene="hadB"
                     /locus_tag="KPK67_000825"
     CDS             complement(4321..5547)
                     /gene="hadB"
                     /locus_tag="KPK67_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBG2457826.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(5562..6362)
                     /gene="hadI"
                     /locus_tag="KPK67_000826"
     CDS             complement(5562..6362)
                     /gene="hadI"
                     /locus_tag="KPK67_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBG2457827.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(6401..7600)
                     /gene="hadA"
                     /locus_tag="KPK67_000827"
     CDS             complement(6401..7600)
                     /gene="hadA"
                     /locus_tag="KPK67_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBG2457828.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            8277..9275
                     /locus_tag="KPK67_000828"
     CDS             8277..9275
                     /locus_tag="KPK67_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBG2457829.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            9637..10164
                     /locus_tag="KPK67_000829"
     CDS             9637..10164
                     /locus_tag="KPK67_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBG2457830.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            10191..10787
                     /locus_tag="KPK67_000830"
     CDS             10191..10787
                     /locus_tag="KPK67_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBG2457831.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(10852..12435)
                     /gene="asnB"
                     /locus_tag="KPK67_000831"
     CDS             complement(10852..12435)
                     /gene="asnB"
                     /locus_tag="KPK67_000831"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG2457832.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(12895..13731)
                     /locus_tag="KPK67_000832"
     CDS             complement(12895..13731)
                     /locus_tag="KPK67_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2457833.1"
                     /translation="MNYIIKKVLNSSVVLVHDTKDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKKFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAIASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(13755..15221)
                     /locus_tag="KPK67_000833"
     CDS             complement(13755..15221)
                     /locus_tag="KPK67_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG2457834.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIAKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIESNSIDFK"
     gene            complement(15224..17086)
                     /locus_tag="KPK67_000834"
     CDS             complement(15224..17086)
                     /locus_tag="KPK67_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2457835.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            complement(17333..18196)
                     /locus_tag="KPK67_000835"
     CDS             complement(17333..18196)
                     /locus_tag="KPK67_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG2457836.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(18251..19183)
                     /locus_tag="KPK67_000836"
     CDS             complement(18251..19183)
                     /locus_tag="KPK67_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBG2457837.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            complement(19269..19820)
                     /locus_tag="KPK67_000837"
     CDS             complement(19269..19820)
                     /locus_tag="KPK67_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2457838.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            complement(20049..20540)
                     /locus_tag="KPK67_000838"
     CDS             complement(20049..20540)
                     /locus_tag="KPK67_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457839.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(20568..21128)
                     /locus_tag="KPK67_000839"
     CDS             complement(20568..21128)
                     /locus_tag="KPK67_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG2457840.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            complement(21280..22437)
                     /locus_tag="KPK67_000840"
     CDS             complement(21280..22437)
                     /locus_tag="KPK67_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG2457841.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(22474..23415)
                     /locus_tag="KPK67_000841"
     CDS             complement(22474..23415)
                     /locus_tag="KPK67_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457842.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            complement(23502..24194)
                     /locus_tag="KPK67_000842"
     CDS             complement(23502..24194)
                     /locus_tag="KPK67_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2457843.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            complement(24302..25114)
                     /locus_tag="KPK67_000843"
     CDS             complement(24302..25114)
                     /locus_tag="KPK67_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBG2457844.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            complement(25165..26076)
                     /locus_tag="KPK67_000844"
     CDS             complement(25165..26076)
                     /locus_tag="KPK67_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2457845.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            complement(26135..26866)
                     /locus_tag="KPK67_000845"
     CDS             complement(26135..26866)
                     /locus_tag="KPK67_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBG2457846.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            26955..27206
                     /locus_tag="KPK67_000846"
     CDS             26955..27206
                     /locus_tag="KPK67_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457847.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            27439..27858
                     /locus_tag="KPK67_000847"
     CDS             27439..27858
                     /locus_tag="KPK67_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457848.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSPINLKSGDIFTFIRLTLLAGRMF"
     gene            27883..32940
                     /locus_tag="KPK67_000848"
     CDS             27883..32940
                     /locus_tag="KPK67_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBG2457849.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSFSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVESSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLVLDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPVHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            33082..>33341
                     /locus_tag="KPK67_000849"
     CDS             33082..>33341
                     /locus_tag="KPK67_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457850.1"
                     /translation="MLLILGNLNLDLDFDYRIIREENDDVDIFIDINYRSLDIDTYGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFAN"
BASE COUNT        11056 a         5682 c         4021 g        12582 t
ORIGIN      
        1 gtattataaa attttattca aacagacttt ttaataactg acaaatacac actagcaata
       61 acttatcaaa gggttaataa ttatgttact aactaataag acttattcaa tcagtaatat
      121 ttatatagtt tgtatctatt aagcttttat agcttctaat aaatcatcaa cgctatctaa
      181 tttttctata agcattggaa ctatttcatg taagtctcca actactccat agtcagcgat
      241 ttctaaaatt ggagctgctg ggtttttgtt tatagcaatt atgaaatctg atgattgcat
      301 acctgctaaa tgttgtattg ctcctgatat accacaagct atatataggt ttggtttaac
      361 agtagttcct gtttgaccta cttggtgaga atggtctatc catccagcat caacagccgc
      421 acgagaagaa ccaactactc cacctaattt gtcagctaat ttcttaagca tttcaaatcc
      481 ttctggtcca cctaatccta gaccacctga tactataaca tttgcatctg taagagatac
      541 taaatctttc ttagttttaa ctgtttctaa aacagtagtt cttatatcat cttgagttat
      601 tttgtagtct aatgctataa cttcaccagt tcttgtttcg tctttttcag ctttatccat
      661 aacaccaggt ctaactgtag acatttgagg tctatggttt ggacaaatga ttgtagccat
      721 tatgtttcca ccaaatgctg gacgagtttg ttttattttc ttatcttctg ggtctatttc
      781 taacttagta cagtcagctg ttaatccagt tccaactctt gaagctattc taggtgctaa
      841 gtctctacct atatgagttg ctccgaaaag cattatttct ggttttattt catctattgc
      901 atcttttatt acttttgtat atgcatcagt tgtatatttt tctaaaagag catcatctgc
      961 aacataaact ttgtcagctc caaaagtgat taattcttta gctaatccat caacatcttt
     1021 tcctaataat attgcacaaa gttctgcatc tacttcttta gctaattttc ttccttctcc
     1081 taataattct ataactactg gagctatttt tccttctctt tgttctgcaa atatccatac
     1141 gtttttataa gaacttaaat cttttatatc attcatagtt tgtactcctc caacttatat
     1201 aatgtgcttt tgttttagac cgattattat tttactagct atttcatcag ctttaccttc
     1261 taagatttct ccttttcctc ttggttcagg agtaaatgat ctaaatactt tagttggtga
     1321 tgcttttaaa ccaacttctt ctttatttac atctaaatca tcaattgtcc aaacttttac
     1381 atcatgttta tatgctttta ctattttatc aacagacatg tatctaggtg tatttaactc
     1441 ttttactgct gttaaaagta caggtgtact tacttttatt aattcgtatc catcttctaa
     1501 ttgtctttga actattatat cttttccttc tattttgaaa tcttgtacat atgtaacttg
     1561 aggaatgtct aatttctctg caatttgtgg tccaacttgt gcagtatctc catctattgc
     1621 ttgtcttcct gcaaatatta tgtcatagtc acctacttta gatattcccg ctgcaatagt
     1681 atttgatgta gcccaagtat cagctcctcc aaatgctcta tcacttaata atattgcatc
     1741 atcagcaccc atagctaaac actctcttag cataaagtct gcttgaggag gtcccattgt
     1801 tataactgtt atagttacat catcatgctc atctttaatt tttaaagctt cctctaatgc
     1861 atttgcatca tctgggttta atatacttgg aactccatct cttataagag tacctgtttc
     1921 tttatttatt cttacttcgt tagtatctgg aacttgtttt acgcaaacta atattttcaa
     1981 agtctatacc tccaatttca ttagattatt tattatttta atacgtttga tgaaattacc
     2041 attttttgaa cttctgatgt tccttcgtat attgatacga ttctagcatc tctgtacatt
     2101 ctttcgattt cataatcttt tataaatcca tatccaccgt gaagttgtaa tgctttataa
     2161 gcaacttcat ttgcaatttc tgatgcatag tatttagcca tagcagattc ttgagcaact
     2221 ctcattcctg catctctctt ttgtgctgca tcataaacta atcctctagc tgcacaaact
     2281 tttgtttcca tgtcagctat agtaaattga gtattttgga atttagctat aggcttacca
     2341 aattgaactc tttgttttgt atatttaaca gcttcatcta aagcaccttg agctattcct
     2401 agagctaagg cagcaacacc tattctacca acttctagag tacccattgc tatcttaaat
     2461 ccttgacctt cttttccaag taagttttct tttggaactt taacgttttc aaatattaag
     2521 tcagaagttt cagttcctct tatacccatc ttatcttcat gagctcctgt tgagaaacca
     2581 tcccatttac tttctactat aaacgctgat gttcctctaa gtcctttact tctatctgta
     2641 actgctatta ctatagctac atcacagaat ggtccattag ttatgaaagt ttttctacca
     2701 tttaatatat agtggtctcc aactaattct gctgttgttt gttgtccacc tgcatcagaa
     2761 ccagctcctg gctcagttaa accaaatgct cctattaatt cacctgttgc tatacctttt
     2821 aagtattttt gtttttgctc ttcagtacca ttgtaaagta ttggtaatga agcaagagaa
     2881 gaaccagctg taacaaaagt agctgttgaa gcacatcttc tagctatttc ttccattact
     2941 atagcgtaag aaacatatcc tgctccccca ccaccatatt cttctgggat attactgctt
     3001 ataaattttg catcagctaa ttttttgatt aattccttag gcattacacc tgttttatcc
     3061 atttctgctg gtaaagtatc taattcttta ctaacaaaat ctcttactgc ttttcttaaa
     3121 agttcttgtt ctttgttata taacatagct tcctccaatt ttcatatttt atagtaaatt
     3181 tattttaatg aacttacgct aaactcatca tctcagcaaa agtttgaata cgagttctgg
     3241 cttgttcatt attctgagtt tgttggtcga tttcaacata taaagtaggt attccactat
     3301 cttctatatc ttttctaact aaaggataat cgtattcttc tgggtcacag aatttcatca
     3361 tacagaagat aacaccatct atatctttct ttttaacttc atctactata agtgacccac
     3421 gttttttctt agggtcataa gctaatgaac atccttctat gtttgaccat tgtcttgcta
     3481 atctttctaa cgcatcatca cctgctggta catctgttct gaattgtctt gtttcttgtg
     3541 ctaagtcgtc agctacaact gatatattgt tgtcttctaa aatgtctaat atatctttag
     3601 aatcagctaa tatacctgtt aataaaactt tctttccaga acagacttct tctggcatag
     3661 catttaattt tgctattaaa tctttcacta gctcagtatg ttcttctttt ctcataaaga
     3721 acccactctt aattactaag cttcttattg atggttttat agtattagaa tgtttataag
     3781 ctacttcaac aaagtctctc atagtttttc tatgttcatt gtaaacttct atactttcat
     3841 gaatttttgc ttcttctatt tcatatccac aaatttcttc aagttctctt tttacgcctt
     3901 tgtactcact gattaagtat tttacaccag cttcaagttt tctattttgt gggtgtacta
     3961 atgatatata ttttatatga ggtactgctg atttccagtt ttgtcctaaa caaattagtg
     4021 tatcacacat acctggtata ataactccag ataattcatc ataagcacct tttaatccat
     4081 attctaaaca tgattgcatt attgaacatg caaatgcagg gaaatattgt ttagctaaat
     4141 ctaattctgt ttgtcctccc catataccaa ctggaagcat tccagctgca tgtataattt
     4201 cttctgggca ataaactggg aaacaaccta tagctttttt accagtttca cttttatatt
     4261 tttttacagc cgcatttgga ttttcaacta cttctttcat tttagataaa atagcttcca
     4321 ttatatctca cctctattaa gtttttttct ttcttccatt acttcaacta aaccttgaat
     4381 tcttgtttca aattgagcat tagtgaaagc tctagggtct gcttggtcac catcaaatcc
     4441 agcatatggt aatccagttt cttcagctgc tcttctttgc atttcatatt gtattaaact
     4501 cataagttta cagcttctgt tcatatgata gaatgctcca tcacatttac cctctactaa
     4561 agaatcaact ctatattttg tcatacggtc taggtttaca ttgttaaaca tagtactata
     4621 tgctactgcc attccatcta aatcattaac ttcatattgt aatgcccaag catgtgggta
     4681 aacactacct gtcatgttaa ctccaaattt agctaatgtt ttcatcttgt atcctatata
     4741 tggccaacaa ggtatacctt ccatcattat tctgtatttt tcttcccctc tgaaagaaga
     4801 tttaccagtt ttcatgttgt cctctaattc ttctataagt aacttaaatg cttctgtagt
     4861 ttctttttta cctctagcac aaactattac agccatgtaa gtaaataagt caaatccatt
     4921 cattggagaa ggagaagaat ctgctggtaa actcatagaa tacttccata gtcttccgtt
     4981 ttcagctgat attttcatta cttcttcaaa cttcttaggg tcaaatttct ttcctgatat
     5041 aatttctagt tgttttatag cttcttcaaa ttgagcttta atataatcta ttcttgattg
     5101 agtaacttcg tcttcattat tgaaagttgt atcaatcatt attaaaggta tatctaattc
     5161 tcttgaaata ttttcatacc attttataac ttggttacat atattgttac agcaaagtag
     5221 gaaatctgga gctggcatat ccaaagcttc acatccacca ttttctaaaa gaccaaaatt
     5281 tgttcttgca tatgcacata ggtcaataga atatccttta gattcagcta tttcacataa
     5341 ttctaaagaa ccttttttag ctgctactcc agctgcttgg ttttctggat ataatacgtt
     5401 taagtcaaat acttctgcta actcttgagg aaatactgat gttgaccaac ctacaggtct
     5461 tccttcttct ttagctttaa atgcatttgc atattgttca gctaataaat cattaattac
     5521 tactctagct tctttttttt cagacatttt catccctctt tttatatatt tttcacttct
     5581 ttttgtgatt cttttgcttc atcaaaagca tataacgctg ctcctaaagc accagttaat
     5641 tgaggtatat caggtacaat aatttctgtg ttgatttctc ttgccatagc tcttacaata
     5701 ccactatttc tagcaactcc accaaccata actacatttc tttgtactcc tattcttttt
     5761 actaggctag aaactctctt tgctactgaa gtatgaatac ctgccactat atcttcaatt
     5821 tttgcatttt cagataaatg tgatataacc tcagactctg caaatactgt acatgtactg
     5881 cttattgata cttcattttg agaattcata gatatacttc cgagttcaga tacatcaacc
     5941 tctattattt ttgccattac atctaaaaat cttcctgtac ctgcagcaca tttgtcattc
     6001 ataagaaagt ttaatagtct tccattatta tctaatttta atacctttgc atcttgacca
     6061 cctatgtcaa taatggttct tgtctctgga attatgaaat ttacccctct agcatgacag
     6121 cttaattcac ttatttgctt atcagcatct gaatagttca ttcttccata tcctgtaact
     6181 acaacttttt taatatcttc tcttgcaaga cctgtcttgc catataattt ttctaaaact
     6241 cttgatggtc cagtagtccc agtaccagag gatattgttt cagaagctac aatatcttcc
     6301 ccattcttta agattactcc ctttgatgca gttgaaccta tatctaatcc cattgtgtac
     6361 atctttagca ttctcctttt aaaaaatcta gaattactaa ttaatatctt acaactttac
     6421 tatctttaaa gttatttatt ttctcttcat cgtagcctaa agattttaat acttctacag
     6481 tatgttgacc tacttttggt gctggtgtat attctttaat tccttcattt ctaaacataa
     6541 ctggagtatt aactaagaca cctgtatttc cgctatcgta tgttttctta aataagaagt
     6601 catttgccca agcttgttcg tcatctaata aatcttcaca gctttgaatt ttttcaaatg
     6661 gtaagtctgc ttcttctaat aaagctgacc actcgtctaa tgttttttct aacatagctt
     6721 ctccaactat cttaactaaa tcttcaacat gattaaccat tgaatctatg ttattatatc
     6781 tatcatcttc taatatatat tctctattta taaccttaca gaatttgcct aaccacttgt
     6841 tgtattgtat taaagctaat tgaatccatc ttccatcttt acatttatat gtagtcatta
     6901 atgggctgtt tggattttct cttgataaag gcatttcatt tccgtattgt gctgttgtta
     6961 tcattgttcc cattccatat atagctgtat ggaaaagact tactgttact ctctcacctt
     7021 taccagtttg agctttttta tgtaatgctg ctaaacttcc tgctgctagt gctagacctg
     7081 catagtggtc accaaatcct gctgctgtat ttgctggaga tgttcctttt tccataacag
     7141 attggctaac tcctcctctt gcgaagtatg cagtatagtc aaatcctggt ttatctttta
     7201 aaggtccttt ttcaccatat cctaatattt gagagaatat taatcctgga tacttatctt
     7261 ttatttggtc ataagctata cccatttttt ctaatgcttg aactctaaca ttagttacaa
     7321 atatgtctgc ttctgataat aatttatgta atatttctac tccttctttt gattttacat
     7381 taatacttac acccttttta tttccatttt ctaattcaaa catagggttt tcatcatctg
     7441 atgctggaga tttaaatgtt ccacccataa ctcttattcc atcaccttct ataggttcaa
     7501 tcttaataac ctctgcaccc cagtcaccta acatttttgc acaacatggt gctgcgatga
     7561 aacttgaaag ttctactact ttaactcctt ctaaaagcat aacgttttcc tccttaatag
     7621 tatcaattat aaaatttcga ttaaatcact cataaaatac ttttttctga tattaactgt
     7681 ctgatgtcaa agttcctaat cttatctaaa ataacttaaa aattattaaa attatttttt
     7741 attgttttat tgtcttacac ctatattgtt aagcatttat cgtgccaact ttatatttaa
     7801 tcaaaatatc gcttgatttg aatgtttttt aacaatatgc aattgaataa tctttttcat
     7861 atcgtggcat cttgccacta tccattgcat tttgctattc tttctatgct tataaaagtt
     7921 ttttttatat atttttttat gaaaaagctt gacatttcaa aaaaagtttt cattgctaaa
     7981 ctcaaaaaca catgttatca ttgtaattaa tcagcaagtt agttaactaa tttcaaactt
     8041 agataatttt ttaataatat ttaatctaaa aaataacaat ttctttgata aattttagag
     8101 taattattac attaaataaa aaattactaa atatcaaaat aacacaggga aaacaatttc
     8161 aaaactaaat ttttaacctt aaaaattttg tataaaaaat ataaccttat atgctacata
     8221 ttatttctaa atgaaaaatt accataataa tttaataatt acaggagggt attgatatga
     8281 aaatactagt atttggagca cgcgattatg aagaaccagt aataaaaaaa tggtctgaag
     8341 aacataagga tgttcaagtg gatatttatc ctgaaaacat gactgaagaa aatgtagtta
     8401 aagctaaagg gtatgatggt atatctatac aacaaactaa ctatatagat aatccttata
     8461 tttatgaaac tttaaaagat gctggggtta aagttatagc ttcaagaact gcaggggttg
     8521 acatgataca ttttgattta gttaatgaaa atggacttat cgttacaaac gttccttctt
     8581 attcacctaa tgcaatagct gaattagctg ttactcaagc tatgaacctt ttaagaaaga
     8641 ctcctctagt aaagaaaaaa gtctgtgaag gtgattaccg ttggatagct gaacttcttg
     8701 gaacagaagt tagatctatt acagttggtg ttataggtac aggaaaaata ggtgctactt
     8761 ctgcaaaatt attcaaaggc ctaggagcta atgtaattgc atttgaccaa tatccaaata
     8821 gtgatttaaa cgatatatta acttacaaag attctttaga agaccttcta aaagaagctg
     8881 accttataac attacatact cctttacttg aaggaacaaa acatatgata aataaagata
     8941 ctctagctat aatgaaggat ggagcttaca tagtaaatac tggccgtggt ggtttaatta
     9001 atacagggga tttaatagaa gcactagagt caggaaaaat tagagctgct gcccttgata
     9061 catttgaaac tgaaggattg ttcttaaaca aaaaaatgaa tcctggagaa ttaactgacc
     9121 cagaaataaa taaacttctt tctatggaac aagttatatt cactcatcac cttggtttct
     9181 tcactagtac agcgattgaa aatatagttt attctagttt aagtagtgct gtagaagtta
     9241 taaaaacagg aactgctact aatagagtaa attagtattt tataaatata ttttttcatt
     9301 aattagctat tatctaacaa aatagccttt aatttcccaa atataagaga gtcagctcag
     9361 tatatattga gtagactctc ttatattatt ttttatatta aacttgtact cttcttattc
     9421 ataccaaatt aaatttatat taacttattc taactactcc tactctatac attttaccta
     9481 tttttacttt tctaattata tacatttgaa tgggtacaat ctgtatttta caaaaaaaac
     9541 acctaacaaa ctatgaaaat tgtatgatta atattaatat atttataaga aaacataaca
     9601 attatgttag ctctggattt ttagggaggg agtatattgg aaaaaatgaa taaagttact
     9661 tctaaaaaaa ctacctatat tgttacttca gcattatttg catccataat atgcctgaca
     9721 atagcatata tattacatat tccagttggt ggaaacaatg gatatgttca tataggagat
     9781 gcttttattt atctagctgc aactattttg ccaacaaact atgctatagc tgcttctgca
     9841 attggtgctg gtctagctga tttatctaca ggtgctgcta tctgggtaat accaactata
     9901 atcataaaac caatacttgt attatttttc acttctaaaa gtgacaagat aataaataag
     9961 agaaatatag ttgcttcggt tgtagcaggt atagtaggat tagttttata catgtttgct
    10021 gaaggtataa ttattggaag ttttacaagt gcatttgtaa tgagcttact tggcttatta
    10081 cagccaatag gaagttttat tgtttttata atcttaggta tggcacttga taaacttgat
    10141 tttaaaaaac gttattttaa ttaagaattt aaatttatgg agggaaaatt atgaatatct
    10201 tatatacaat tgaaaatagt atctatgtaa atataactaa tacatgtcca tgtagttgtg
    10261 ttttttgtat aagaaacgaa aaagatgaag ttgcaaatag tggtagttta tggttggaac
    10321 atgagccaag tgtagacgaa gtaaaagaag ccttcaataa atacaattta gacgactatg
    10381 atgaaatcgt attttgtgga tatggcgaac cattaatgag aataaatgaa ttgatagagg
    10441 tagcaaagtt tataaaagaa aaaagtagca taaagataag aataaataca aatggtctaa
    10501 gtgatttaat acataacaaa aaaacagcta ttctgttaaa aaatgttata gatgcagtat
    10561 caataagtct taatgcccca aataaagaag cttataatag agttactcaa ccaaagtttg
    10621 gagaaaaatc atttgattat atgttagatt ttgcaagaga ttgcaaaaaa tatataaaag
    10681 aagtagcttt ttcagtagta gatgaaatat ctccagaaga aatagaagaa tctaaacaac
    10741 tggcaaaaaa acttgatata ccattaagag ttagacataa gaattagcaa gtaaaattat
    10801 aaaacattaa gaatcacctg aatggtcttt caggtgattc ttcctcttgt attaacattt
    10861 tacaaaatag atttcatacc acactaggaa tgtgtaaatc atccaaatct ttctccaatt
    10921 atcagagttt ccactaatgt agtcattaaa aatctcatta atctcatcta tgttaaagaa
    10981 tttatctgct atctcactat tgaatttatc tcggatatcc ttattatatt ggtcatcagc
    11041 catccatata cgaattggca caataaatcc taatttattt ctaaaagcaa tctcttctgg
    11101 taatgttttt gatgcagctg tacgaaaggc aattttattc tcttttcctt tcactttata
    11161 ttctgatggt attctggatg caatatcaaa tattcttgta tctgtaagag gcattctgac
    11221 ttccaatcca actgcagtac tcattttatc tatattaaaa taaatatctc cttccaacca
    11281 tatttgaata tctacatttg acatgttagt aagtgtatct agttcttctg tttcttcata
    11341 aattcctttt accacttcaa taggaagcac atcaggattg tatttcttaa gaatttttct
    11401 tttctcaccc tcattcataa tgtttgtatt tccaatataa aatgtcttca aattctctcc
    11461 ataacactct gccttatggt atatattata tccaccaaaa aactcatctg ctccttctcc
    11521 agagtaacag atatttgtgt tttcagctgc tgccttgcat ccaatagcaa aaacgatagc
    11581 agatgcatct ccagttggtt gctccatatg gtacattaca taaggtactg acgcaaaata
    11641 ctcttcaggt gtaattatat atctcgaatt tttacaccca agaatattag ctgtctgttg
    11701 tgctaatttt gattcatcaa aacgctcttc ctcataacca catgaacctg ttttttctat
    11761 atctgacatt gccaatatat aagatgaatc aacacctcct gataggaata actctgcagt
    11821 ttcattctct gattttacct ctggcataat tttctgaagt gttgtatgaa tttcatccgt
    11881 ccattcctca atagacttgc tgtggtcagg atgaaattca ggtttccaat aacgaccaat
    11941 acatactgtt ttattctgat agacaaggta atgtcctggc ataagcttct ttactccacg
    12001 gaagaatgta ttttcacctg ctacatatgt gaaagacata taaatctgta acatgtcttc
    12061 atttaactct ttcacaaatc ctggttgctc cataatctta cgaatagatg aaccatagag
    12121 aagcttccca ttttcagtct cataataata gaaaggtttt actccaaact ggtctcttag
    12181 gcagaagagt ttcttttcca cagtatccca taatgcaaaa gcaaacattc catgcatgtg
    12241 atttgccata tcatatcccc atgtcttata tgcttccact aaaattgctc gttcacgctc
    12301 atctctacat aatgaagaat cgattccaag ttcttcacag agaactttcc agtttcgaat
    12361 atgtccattg tattctagaa tttttatcat atatgtgtct tttcgtgtta aatacttttc
    12421 ttctaaattt atcatatgtg tatcctttcg tattaaaaca tttaacttct aaatttatta
    12481 aatgtttata tatatttatt cacttataat cactgaaaaa tcgacttttt tcaaagtaaa
    12541 ttctttttga attagtacta atctgctctt tattaatggc ataaatatga ttatcaagta
    12601 ttatcagtgt tataacattg aattttttat tcttcataaa tattgccccc tttatattac
    12661 caactaaata taatatatct tatgttactt acatattctt taagaaattc tgaagatttt
    12721 ataaagtttt ctgtattata attaattaaa aataaaattc tagttataaa acatattttc
    12781 cattaaacaa aaaaatatgt ctcagtttct ttatatactt taccttcttt caattttcta
    12841 ccatgtagac gtataagata atattagtaa ttcactctat tgctgtaatt tactctattg
    12901 cttaactcgt gcgatatgga caaccaagaa agctatttcc tcatttgtta acttttttcc
    12961 atatttcttt tctaaaaaat ctctaacttt taatgcaatt aacatttctt ttggatttga
    13021 ccttttcatt tgattaaata ataaatcatc accactgctt aacatactat ctgaaaaaaa
    13081 tctttccaca aaaaatttta tatgtgttat aaatctcata taatgcatac tctttttatc
    13141 taaggttcta ttcaaagaaa aaacaaaaat attaattatt tctccaatta ctttggcata
    13201 cttaatcgta tcatatgaat ttccatctga agctatagca tttgcaaaat ggaaagcaat
    13261 gttagctgct tcttcctctg gtaattgaat gcccaatctc tcctctacca tataaattgc
    13321 aaccattcca accttaaact catttggata ataatttttt atttcccaaa atactcgatt
    13381 tgtaattttt attcctttac gcatcctttc aattgcaaag ttaaaatggt ctgctaatat
    13441 taagtataag tttttattaa aagatgagtt caataacttt tctgcctcta ctattatttc
    13501 ctgtgttatc tttaaaatgt cgacaggaat atcatccatt aattctaaaa atttttttga
    13561 ttttgtattt tcaacaggga caaacatttg attatcattg gaaccacaaa tatattctcc
    13621 tgttttttta ccatatccaa ttcctttccc tagcaaaata aattcattat cctttgtgtc
    13681 atgaactaaa acaacgctcg agttcaaaac tttctttatt atgtagttca ttttatttgc
    13741 tcaccttcta ttggtcattt aaaatctata ctattacttt caattacctt tttataccaa
    13801 taaaaactat ctttcttata tcgattatat gtcccctttc catagtcatc agcatccaca
    13861 taaatatatc cataacgttt tgacatttgt ttcgttgatt cactgaccaa atcaatacaa
    13921 ccccatgaag tatatcccca aagttcaaca ccatcttcaa taatcgcatc taacatacat
    13981 ttaaagtgtt cttttaaata attaattcta tattggtcat gaactttttt atcttcagtt
    14041 aaaatatctt tggctcccaa tccattttca actataaata aaggctttct gtatctatca
    14101 tataattcaa tcaaagatat acgtagacct attgaatcaa tttgccaacc ccattcactc
    14161 atttctaaat aaggattttt tacagctaat aacgtattcc caggtgtaat atctaatcca
    14221 gtcgtatcag ctgcctcaca agaagacata tagtaagaga atgaaataaa atctactgga
    14281 tactttttca taatttcatc atcatgttct tcttttgcta tttgaatgtt attattttta
    14341 aaatacgaca gtaaatatac tggatactca ccaaaaacct gtgtatcact atatgcataa
    14401 atacttctca ttttttgttg agttgccaaa acatcttctg gtctacatgt atatggataa
    14461 tacgttaatt ttgttaacat acaccctact tttgaatttg gaataatctc atggcaaatt
    14521 tttgttgcaa gggcactagc tacaaattga tgatgcatag cttgaaaaat aacttcctca
    14581 aagtttttat ccttaaaacg gtctcgaact aaaccaccag tagtgtacgg atgacgaatc
    14641 atagaatcaa cttcattaaa tgtaagccaa tatttcactt tgtttttata tctttcacag
    14701 atagtttcta catacctaac aaacatattg ataacttgtc gtgaatacca tccatcatag
    14761 tttaaaacga tatttaatgg tggttcataa tgtgacatag taacaagtgg ttcaattcca
    14821 taacttatca actcatcaaa tacatcatca taaaactgta aaccttcttc atttggctct
    14881 ttatcatctc catttggaaa aatacgagac caagcaatac tcattcgata tgtcttaaat
    14941 cccatttctg ccattagagc aatatcttct ttatagtgat gataaaaatc actaccatga
    15001 cgttttggat aatttacttc atcttcagga tgtgctaatc cttcttctat atcttttgtt
    15061 gtgatttcat tattagattt ataatctttc acatctgcat caaagtgtgc ctttgctaca
    15121 tctgatacag accacccttt accacctaaa ttccaaccac cttcaaattg attagcagct
    15181 gtagctcctc cccaaagaaa tccttctgga aactttctca tatttatatt cctcccttta
    15241 catcaaacaa gatacttcct ttttttacat tgccataaga ctggtttaac actttataag
    15301 cataatctgc tccattattg tttacaatca taacagttga atctaaatta gcatgtgaga
    15361 ttttgtctaa atcaaactga attaataaat ctccttcttt tacattatcg cctgtcttaa
    15421 caaatgtttt aaatccatct ccattcatac ttacagtatt tattccaata tgaatcaaaa
    15481 tctcagcccc attttttgtt cttaaaccaa ttgcatgttt tgtttcaaat aacatcacaa
    15541 cttctgaatc acaaggtgcg tataaatttc cattggtcgg tataattgca actccatctc
    15601 ccaaagtctt acttgcaaat agttcatcat taaccttact taaatctatt acttttcctt
    15661 ctactggagt tactaagata tttacttctt tttcttcttc tagctcttct tcaacaacat
    15721 ctccaattga tgactctact gtatttgtat cttccttcca aataatcagt gtaataataa
    15781 acgagcctaa tattgcaata acaaaaccaa tacttgcaaa aatcaaattg tttccattgt
    15841 ttgggtcaat aaacatactc atactggcaa gccctggtcc aacaattaca aatcctttta
    15901 ctgcaaataa tcctaaaaat gtaccagaaa ttgcacttga gaatacaaca cctaacattg
    15961 ctcttttatg ttgtaaagta ataccatata aagctggctc tgtaattccc atcaaggcag
    16021 aaattcctgc agataatgct gtttgtttta atgtttcatc ttttgttcgc aaggctactg
    16081 caaaacaagc accactttca gaaatattgt gggctaatga agatgtcata tataaagctt
    16141 cataacccaa ctttccaatt gtattaattg aataaggtat tagtgctttg tgcattcctg
    16201 ttgcaaccat aaatggaaga aaagcagcaa gtaatcctac aactaagaat cccattttgt
    16261 tatataagaa taaaataata gttgtaaaaa ttgtaccaac gttatatcct aaaggaccta
    16321 aaatagttag agtaattgga acagtaattg ccaaagacat cataggaaca aagaaagttc
    16381 gaattggttt tggtgaatac ttgttaaaaa ttttctccat tgttgccaag aaagacacac
    16441 ataagatagc cgggaaaacc tgtgcattat aggcaatgtt tggaattgta aatccaaata
    16501 gttcgacacc ttcacttaaa gcagttgttg ttgctggaag caataagtat cctacagcgg
    16561 caatagctac taaacgatta catttcaata cattggcagt agttacagct accataagag
    16621 gtaggaaata gaatgttgca tctgaaatag caactaataa cgtatatata gtagaatctg
    16681 attttaaaat attaaacatt gctaataaca tcaacagtga ttttaaaata ccagctcctg
    16741 caatagcaaa aatcagtggt tgaaaaatat taataataaa ttctataaat cttgttccta
    16801 agtttatttt ttcttcagtt tttacagtac tatcatttgt atctaaaggg caaactttta
    16861 aaatttcatc atacacatca acaacactat ttccaatgac aacttgaaat tgtgccccat
    16921 taactatact cataacacct tgtacttttt ttaaagcatc ctgattgact ttacttctat
    16981 caatcaactt aaagcgtagt ctagtcgaac agtgttctaa agctaaaata ttttttgaac
    17041 caccaacttg ttcaataatg tcaatggcag tttttttata attcatcttg taatctcctt
    17101 ctccataatt ttttatgaat ggccattcaa gatgcaaaaa aggcagaacc tatataaaca
    17161 ccttgaaaaa tcaaagcaat atattcgatc ctgcctgcat taccagtaac atgtcttcta
    17221 tttatactat tttgtattat tatattaaca tttgcctaaa agaattgtca atactttttt
    17281 aaattttctg atactaacta atagcacctt tataattcat tttttatatc aattaaagta
    17341 acagtagcca cccaccaatt agtatcaaaa ttgaacctgg tattttttct tttatatttt
    17401 ctttgaatat aaaatgagca agtattaaag ttattatcat agatacttga gaaaatggct
    17461 ctgctatact aagttctaga ttatttaaag caattaataa tgctaaataa gaatatccat
    17521 taatagcacc acttaaaatt gaaagcccct ttttattagt aaaaatctca caaattattt
    17581 tttgtttctt tttaaatgct aaaaagataa ataaatttaa acctacgaaa aaatacagta
    17641 tagttgaata taatatagga gatacttgta caaaaaaagc cttatccaat actcttccta
    17701 ctgactgcaa aaatatgtat aaaaacatta atctacatgg taaattgtta aaaattacag
    17761 agacattctt taaaaaagag cttacttttt ttaatataaa cactccacca atcataatta
    17821 tgattcctgc aacttttgct aaacttaaac cttcacctaa aaatataact gataaaagca
    17881 gtaaaaacaa tgaatttaaa ctatttattg gagtaacaag tgaaacttcc ccagttgcta
    17941 aagacgttac atacgctact gaaccaattg aatagactaa actacttata taacatggta
    18001 ttaaaaacga ccaatcacta actctatcca ttatgctaaa tggaagtaaa catactcccc
    18061 ctataaagaa aaatagaaat gatgaagcta tatttttata aatatctcct tcttcatcac
    18121 ctaataatct tacaactact ctttcaaaag ctaatagcac tactctaatt aataatgcaa
    18181 aataagataa tatcattttt gacctcgctt aaaaattaat ccgacttatt gttatattaa
    18241 ctgtataaga ctaccatata ggtaagtctt ttggattttt taatatttta tgtttaaata
    18301 caggtacatt aagtttttca cctagtttac ggcattgctc tttataggta ccttttaatt
    18361 tatcatgaaa aactatacaa tcaattcctc cttcaatact atttatacac ttttctagtt
    18421 tctcaaatcc agattcatca tcatacattc cacttacaat ccattgattt ggcaatacat
    18481 gtccaattga tatgttatat tctttatttt ttataatatc tgagaaagat ttctcaaaat
    18541 gtacccctcc aacacataac aaagtcttca aaggttcatc acaatcaaat actctaatta
    18601 atgattttgc caatacctgt attgctatac tattattaaa actatcacta ctacttccta
    18661 tctcaatatc caacataggt acagaatatt ctgtaattaa tttagattct tcaccatatg
    18721 ttgttccaga ccagtgagtt gcttctgtta atgtagtaaa ttcatcaagt agattttcat
    18781 tttttatatc ctcaatagca ttgattagat ttttaaagta tcttggattt gaatttccaa
    18841 attctcctgt tggaacatct ccaattgtat gagctgttag gatatgttct ggagcatttt
    18901 ctccctcatg ccaatttaca actcctgcaa aatcataatc tctgaaatgt ctatttaaaa
    18961 ttggaagata atatttataa ttatgactta ttacatcttt tgtttttaca aaatgaaata
    19021 agttattgtt tttatcctta aactctaata caaaattatc atcaataaca atatcagttt
    19081 gatttaaatc cattatttct tcacaacatt ttatcacatt actggctact gggtctttct
    19141 ttaaatcact acaaaagaaa aaaactgctt ttttattatt catccataaa tcacactcct
    19201 ataacttaaa tataatatat cttcatacct aaaatcatct aaatatcact ctaattccat
    19261 taaattattt atatataata tactaccata ctcattttag ctataccttt gcctgagttt
    19321 ctatatatat gtgatacatc agctttaaat cttatagaat ttccactaga tacaatgtaa
    19381 tcttctccat ttattttaat ggttacttcg ccactaaaaa cagttataaa ctcttgtgtt
    19441 cctggaagtt cagcatttga atctaaattt ccaccttctt ctatttcaat cataaatatc
    19501 tcaaatcttc ttttggagtc aaatggaaat attggataac ttatatactt tccattatct
    19561 tcaataaaat ggtctatatc acttttttga atcaattcta tgtcactttc ctgcctctcc
    19621 atcaatgatg taaatgagat ttttagtcca ttagttattt tcttcaaagt agatatagta
    19681 ggatttacct cacacttttc aatttgagat agcatactct tacttacacc actaagttgt
    19741 gcaatcttgt ctaaactaag atgtttttct ttccttatct tttttaaatt ttcagcaatt
    19801 atttcattta aactttgcat aaataccccc taatacatac taatataaga taaattattt
    19861 tgattattaa ataaacttag taatttaata gaaacttttt atccattata atgtacatta
    19921 tgtttaatat acattacttt ttttagaatg taaatagttt tctgaaattt cgtgaaaatg
    19981 aataaataaa aaactagtaa cctatatttt tacaaggtta ctagctttta ttataataat
    20041 atttcatttt aactataaaa cttagatgca cctataagct gtctcttaga aacaatctta
    20101 gctaggtttg ctaatgagga aatttttata tctttaaaag aaactaaaaa tacaaactta
    20161 aaatctgtaa attcttgatt tatattgtat atatcaaaat aacatctcaa attatcaaat
    20221 aagtaattat cttgtaagtc tgcttctatc tcatcagtag catcttttaa atacgctctt
    20281 acatcttcaa ataaaacact gaaataagac tcatctattt cttcacctaa ctcttccaat
    20341 atttcttcta tatctaaatc atccatatca aaagaatttc ctgtcaactg gcaatcaaat
    20401 accaatatat tttcttgatt tatagtattt gatatatctt gtacatattc gaagtctgga
    20461 cgtaattcta aaatgtcttt tatttcactg ctctttacat ggatttttcc ttttctattt
    20521 tcaattttta aatctatcat atgctccccc tttcaatcta caaatcttta aataccttgt
    20581 tttataatct tttcaatatc tatccaatta ctaactctaa ctatattttg atgtttaaca
    20641 tctttattat agttagtatc cagaagtatt actttaacac catcatcagc tagttgcact
    20701 gagtttaaag gattatcttc tataaatatg tcacaactca gttctcttgc cttttcaact
    20761 ttataatcac ttcctaataa gtaaacatct atatctgaaa atccttgtct cgatagccaa
    20821 tttttagtta catcagttaa acactcatgt ctagccgtta caaaagataa attatgtcct
    20881 ttagataatg tgctaattac ctctgtagca ccctctacaa tctttgcttc atcatatgag
    20941 tgtatatact tattattaaa ctccgcatac acatctctta ttcccgaacc atacaattct
    21001 tcccaattat gtgttataca ctctgcttca gttatatttt tattaaagat atcatttagg
    21061 tatggaagaa aatgataagg gcttgttatt gttccatcta tatcaataca tatattcaat
    21121 ttgttcattt ttacctccat gtaacattta ttgatttgat taatcaaata ttatagcaca
    21181 gctttaatta taaatcaaaa ctctggatat gactatacct atataatacc aaaaagcttg
    21241 catatttaag caagcttttc gtaaatttta catttttaac taatatataa gtttttctaa
    21301 ggttggttcc actccaaatt gtgatttcag ttctttatat tcttttaatg ctttgtcatt
    21361 attcgtttta gtttttatcg ctcttatcat taaatttttt ggtgtatcaa gtggagatat
    21421 gtattctact acagaagtat catatccatt gccctccaat aacaatgttc ttaaaccatc
    21481 agttatcaaa tctgcaaatc tagctttaaa tacaccatgt tttaatattg gttccatagc
    21541 ttcatatcta tattgcccta ataactcctt atgacaacat ggtactacta ctatagcttc
    21601 agattttgct ctaattccta gacctattgc catatctgta gctgtatcac aagcatgtag
    21661 acttattaca aggtcaacat ccctattagg tgtataatta gtcaaatcct cttttataaa
    21721 tgacatattt ttataaccca agtttttagc catatttttt gatgcttcta taactacatc
    21781 tgaataatct attcctataa aataacagtt tttcttcaat acttctttta tataaaaatt
    21841 taagacaaag cttaagtaag attttccaca agcacaatcc aagatagtta tacagtcttt
    21901 atcctttatt tcttttaaaa tactatctat caattcaaca aaatggtcta tctgattgta
    21961 ttttcttatt ttatcatttt ttatcttacc atcttttgta agtactccaa tttcttttaa
    22021 aagtgcattt gcttgtccta cttttacata ataatctctg ttttttattt gagatgccgt
    22081 aaattcatct attttaggta cttcttcctt attatctaaa tattttacat ttaccttctt
    22141 attgtctcct tcaattacaa caatagttcc tcgctcaaca tatttaaata ctaatccatc
    22201 ataattttct gcctctttag aaatattctt aagcaactca ttaaacgtat ataaatctga
    22261 ttttccttta aaattaaatt tatatttatt atcttcctct attgcaattc ctttaaactc
    22321 ttttcttcca gatttaaaaa tcacctctaa ctcaataaaa aaatccctat tatcaaggtt
    22381 tctttgagta atccccataa aaaacatatt tagttttcct atactctgtt tattcatcat
    22441 aatctccctt ttaacttata tccttaaatc ttattaatta atattatttg tttctgaatt
    22501 atttatttct tcttttaaat cgttcaatct atttttaaaa ctttctatct gcttatctga
    22561 taaatcaaga tattctgtta tcttccctat atatttaggg cttttagtat ttattaattt
    22621 caaagcactt tccatagatt tttctctaag ctcatatgct ttctttacgt catctctagc
    22681 attactcaat gtttctgtat acttctcctt agaaagttct ggaatctgca tattttcata
    22741 ttcttttaca acttctttac acttgtttct ggcatttgta acatcttttt ttatactaga
    22801 taatttagtt atattttttg ctatatcctt agtgtaaggt atacattctc tcattggaac
    22861 taaagctgct ctttctatat tataaatttc tttcttaaaa attcctatac tttccatatc
    22921 tatttttttt atatcttcac tatttttttc tttacttgta tatttattat tttcttttgt
    22981 attatcttct tgtttttctg atttattatt cactgaaaca ttactatcag aattattctt
    23041 ttcttttata tcattacttc cttcttcatt actttcttta ttttgtatat ttctatcttg
    23101 tttgttgttt aaatttttat tatttgatat ttcttcattc tttttattat cctgttttaa
    23161 atcagtactt tctttttcct taaaatttga tacatacttt tcatcatatg tctcatcaga
    23221 aatataattg tgataataca tagactcttt tattttatta agtccactca tataaatatt
    23281 acttatcatt attgttgtag ctaataccac ataaatacta ctgtagatac aagacctctt
    23341 aaaattcttg taatttctga atatcactac taaaggaata agatataaaa atataaaaac
    23401 taatatccac ctcataagtc gtgtctcctt tctcaaaacc gaaatccctc aaatatttat
    23461 ttttaatcat gtatcttata gtatttacca atttatgttt tttaatcgtg aacttttatt
    23521 aaattaaatt ctgtatagtc acaagatgct aatgtatatt ctactccatt tcgaatcccc
    23581 ttcaatccca ttttaaaaga ttctttacca tgcaagtgac cataaatcac cttttcaaca
    23641 ttatattctt caaataactt tgtaaataaa gattcttcca atttatcatt cgtaggagga
    23701 taatgagtaa tcacaattat ttttgagtgg ccacttttac gagcagattc taatgatagt
    23761 ctaagtctat gctcttctct tttataaact ttttcatcag tctcatcaaa tttaacatca
    23821 ttaggacata tccagcccct acctccacaa attgcataat ctttatattc ataaaaattt
    23881 gtctgaataa aacgcatgtc ttcatatagc ttatttaaac ttgtaacggt tgtccaccag
    23941 taatcatgat ttccttttat aagtattttc tgacctggaa gtttgcttat tatatctaaa
    24001 tcttttttag cttccgataa attaattccc caagatgtat ctcctagtac taagaccata
    24061 tcttcttcct taacaacttc tttccaatta tctataattt tcttttcatg accatcccaa
    24121 ttacttccaa agatattcat tggcttattg actgatgttg aaaaatgtaa atcacctata
    24181 gcatacaaac tcataatatc attcctttct taattacact aaataggctg tctcttttta
    24241 gaaattattt tccattaaga ggcagcccca ttaatattta gcaaattttc tacaagtagt
    24301 tttactgaac tactttatca tctaccttag tatccttata ttgctctagt aatatatcta
    24361 tttcttgttt taattccaaa atttcagtta tatttttatc tgtttcagtt ctatcaagtt
    24421 ttctttggta ctgcatagct ttatgtgtat cacccaagtc atataactct tgtattctat
    24481 ttagagtatc tctaatagct atagacttca tttggtacat agcctgagct ttcattatgt
    24541 cctctcttat ttcactcatt tcttggtcta aaagaaaggc agcatcagat gctcttctaa
    24601 gcctttcagc aagagtctca ggtatgtaat ggtcatattt atattttaat gaatgttcta
    24661 ttgtagccca gaaattcatt gcaagagttc ttatttgtat ttcacatata atttcctttg
    24721 aacccgcaat ggagtttata gggtatttta ttataacatg atagcttcta taaccactat
    24781 ctttgtaatt tgtaatataa tctttttctc ctattatagt catatcattt cttactttta
    24841 tcaaatcaac tatagcatat atatcctcaa caaactggca cattattctt ataccagcaa
    24901 tatcatcaat ttcagtttct atatcttttg catttatcct ttttaactta gatattatgg
    24961 aagatatttt ttttgttcta cctgtgacaa actctatggg agaatattca ccttttgcta
    25021 aatattcttt tcttatattt ttaaatttta ttttcaattc ctctactgca tgttcataag
    25081 gtgctagtat ctcattccat ttttcgtatt ccatattgct caccctttat ttgtttttta
    25141 tgaccttctt tattttatca taatttattt tgtttttgta tagtaaagca attctccatt
    25201 ttccaatata taccctattt cttctaaatc aactaagtaa cttatgacag atactaacga
    25261 agatttaaaa aaatgatact ctttataatt attgcttaaa ttattgttat ttataatgtt
    25321 ttttaataaa atttctaaag atttcggttc tacaagcaac tctcttattt gatataaata
    25381 tttttttaaa gcattcaaat gtttttctat taatttataa gaatcttctt tcgatataac
    25441 ttgcttgcca tgccctaaaa ctaagtactc aaaatctata ttttttaatt tttctaatga
    25501 atttatttgt tcttgtatat catatagaaa caagaaatcg tattttgata gaatttcatc
    25561 tcctatcaat aaatcgccaa caaaaatcac attatccttc gtcaaaacag ctatactacc
    25621 aggtgtatga ccctgaaatt caattatatc aaacatttca ttgtttaatt caatttttcc
    25681 agcttctata gatttatcta tttttatatc atccaacatt ttatttttta agatattatc
    25741 cataaataca ttgctctttc ccccaacaat atactttgaa aacaattctg gattttctat
    25801 atataattta tcgtattcag atgacattat acacacatct ttataatatt ctttaaattg
    25861 attacaagct ccatagtggt catcatgacc atgagtattt ataatatatc taacctgtat
    25921 attattttca tctaatactt tatttactct cctaggtctt gaaccacata accctgggtc
    25981 tatcataagt gctgtgttat cttcaaatac atatattcct gtattcgtgc caccttttat
    26041 aaaaaaggta tttcctttta cttttgtaag attcaccttc ttcacctcat tttgacaata
    26101 tttatgatac aatttattac ttactaatat aaaactatac aagaacaatc ctttttattc
    26161 tatctatagt tctttttctt attttgtatt ctttagatat ccttattttc acattatatg
    26221 ctaaatttct ataaagtaat gactctataa aatattcaac tatacctcta tccctaaagt
    26281 ctatatctat atttccactt gaataaatat ttgagtctaa tatcataagc tcatcaaaaa
    26341 agcttaatct cttagattta cttaaataat ctttatttgg aataaatcta tcacaataat
    26401 ctatcctgta atttactact tcatcattta tttcaaatcc atattgcttt ttcattaact
    26461 ctctatagtt attatttttc tcaataacat tatctaaatt tttattataa tcctcttttt
    26521 tccaatgtcc aaacatccta caatttaatg gtctaacttc atatattaaa catctattat
    26581 tagtatcttt aaatggacat gggttctttc ttgtatactc ctcaaaataa taatctaata
    26641 ttttacttat acagcttttt tttaaatcaa ctctatcttc aagataacaa aatatattca
    26701 aaaactcaat taaatttata cctactgact ccatacaaca atttccacat ccagaacaat
    26761 ttcctactgg caaggactta tatacatcat ttagtttgtc aaagagctta tttttaatgg
    26821 aataatctat acaatttaaa atatctttat tttttatact agtcattatt attactcctt
    26881 gttataatta ttaagttaat acaaacaatt attataacat attttttaaa tttaatcgca
    26941 tatggaggta ttttatgaat agatatacaa aaataattaa tatgatggga agttattaca
    27001 caaaagactt tgaaaaagaa aaaaagaatg taataaaagt tagagaagtt aaagaagata
    27061 cagttagaaa atttttctta caaggtgact gtgaagttct tgtagtattt gaagacactg
    27121 ggaaagaaat acttattgat gacttttctc ctgaagaaga tatcaaaaaa tatttaggag
    27181 caaaatttat aaataaaaaa agatagttat caaaaataat tctaaaatta aaatttaata
    27241 aatgtacagt tataattcta tataattata gttaaaagtt atcttttatt gcttttttag
    27301 actttaagat agcttttttt ctattttttg taaaataatt ccataattca cattaataat
    27361 tcacaaaatg atataatata acaaatagat aaattttaaa tttctaaata cataattaat
    27421 attttgcgag gtgattttat gcaaatttat tttagagtta aggctgttgg caaacgcaaa
    27481 cctatgctag aacttacccc ttttgatgta tctgacaaca tcaattgcct aaaagaagct
    27541 atctctgaaa ttgtatctaa aaatgttctt gaatataatg agaaaatccc agaaaaatca
    27601 ataataaact tcttaactaa tgaagaaatt gaatctcaag ctgatattgg aaaagttgga
    27661 tttggctcta tttacaatga gaataaacaa tctgtagaaa aggcaataga agtagcgtta
    27721 caggcatttg aagatggaat ctacaaagtg cttataaatg aagatgttat tgaaaaactc
    27781 gattccccta taaatctgaa aagtggagat atatttactt ttataagact taccttgctt
    27841 gctggaagaa tgttttaaaa taaataattt ggaggtacat aaatgttaga acgtactaga
    27901 gtagaaaaaa atcctaaaat aattgaaaat tttaaaaata attttactaa atcttcttca
    27961 aaacagtact acgacttagc aaagtctctt tgggactgca tagaaaatag ttcatactat
    28021 tatagagaac ctgaagacaa attcttaaat atgataaaga aatactacaa ttgccactac
    28081 tacgaaatta aaaatatgga cgagctcctt tctggacctt taaaaaatgc attagaatta
    28141 atatttactt ctgaacaagt agaagaactt tgccaattaa ttaaaagaaa gatggattat
    28201 acatattcag gaggatatta tcgtaaaggg tatcgctcaa ataaacttgt ggattatttt
    28261 agaggtatta taaggtttat ttcaaactat gttgatttaa tttattatga tttttcagtt
    28321 atagattatc ttactcttcc aaataaggtc ttgtactatg actctataat gagtgataaa
    28381 atagcactag aaatagacaa aggaaatcaa aaagtaataa gtgctttaga agaagtaata
    28441 tttggagaaa atcaaactgc tctacttaca agacaaatga taattggtat catgaagagt
    28501 catagtgata aatgctatga aatgctttct aaactccttt tggcagccaa acaacaagaa
    28561 ggacttagac agcaaattgt agagagttta gatgagggta caatttctgc atcaatatac
    28621 atgttaaaaa ctattttgga caacaattta gagcgattta gttctgttat aagagccttg
    28681 gatacatgga caggacttgg tttttcagac caaaaacctg gaataatacg taaatgttta
    28741 gaactttcat ataaatcatt gactgatgaa aagttccgta tagaatcaat taatagcaat
    28801 gataatcttg aaatttattt tgccttatgg gctactgcaa cttacaacat atatgactct
    28861 ttctctctta ttgaaaactt actttcttgt caggaaaaat ataaaaaact tgtagctctt
    28921 tactttttat cgcatacaga aagtacatat tttaaacatt ctatagcatc taaaaacatt
    28981 aagggtgatg atttagagat actagcatgg gtaattacaa atttctatgt agaaagttct
    29041 gttggctact actatagttg gcaaaacaga gaaactaatt ttgacttgtc aaacactcct
    29101 attgacaaag actttgatgc tagaaaagaa caatttgaaa gtttaaaaca tactttagaa
    29161 attttaaatt caaagacaaa atcattttca ggaagtgtat ttccatggac ttggattgaa
    29221 atttcttctc atgaaatact taaatgtatg atgactttaa cagcatacga tatgaatgct
    29281 caatttatag atgtgcttat ggaaaattgt catcttatga gttcagattt aagagcttca
    29341 cttatagtca attttgtaaa tgaaccaaag acagatagac aacgtgaatt tttgttggaa
    29401 tgtttaagtg ataagagtgt ttccaataga gaaacggtat tgagtctact tagaaagctt
    29461 gagctaacag atgtagaatt agataagata accaatctat tgtctttaaa gacaagctct
    29521 attagacaaa cagtaataaa aattttactt gaacaagatg attcaaattt ggaaaatatt
    29581 gcttcttcac tgataaaatc ttcaaattct caaaaatgcc ttggaggtct tgaaattgta
    29641 aacttattaa aagacaatac tgctagacaa gaactatata ataagcttat tttacttata
    29701 gaagatattg ataaatctaa aaagaataca tctcaagttc aaatactaat tgataatttg
    29761 ttaggtaaag caaaaaatga ttactgcaaa gaaaatggtt ttggaatata taaccctgaa
    29821 aacaaagttc aaattccaga acaatttgat tttaaagatg attctaatag ctcatcaaag
    29881 cttaataaaa ttatctcaaa tataagttta tctactcgtg aggattctgt ttctgtagca
    29941 acaaaaaaag aagcaaaaaa aattctcgat acggatataa ataaaataat aaaagtttat
    30001 gaaaaatttg atgagcttat tactgaaaat gcagattatg aatatgaagt tgaaagatgg
    30061 tcaggtaatg tagacaaagt acttttaggt tattctactt ataacttgac tcctattaaa
    30121 tataaagttt ctaatgatac tcttgataat tatccattag cagaactttg gagagaaact
    30181 agtaaaaagc ttgatttaga ttacaaatta gtacttgata tcttatttta tagtataagt
    30241 acatgtggat ataatgaaac agaaaaaagt tggttttcat ctatatattc aaatgtcctt
    30301 gactctaaaa aacaaaataa aatatataca aaacttgaaa aacttaaatt taatggactt
    30361 acaagaaaac ttttaagagt tttactaaat gagtttgacc ataaagaaat atttaactat
    30421 tgtttagaaa tatactataa acttagaaac ttattgacta tagaacaatg taaaaaacaa
    30481 agagttgaat ttactaaaaa ctattacaga caaaatgaat actgccctgc tttaaatgcc
    30541 cctattttaa actattggct tgataaagca cattcgttta tttatgatga tatttctttt
    30601 actaagtttt tctacactac gttttcaaat tatcagttgc tagaatataa gtttcctttc
    30661 tatactagca taaatgtgtt tgctcgtgca catgaattag gattaatgac tgaagagcaa
    30721 gttaatagag aaatgataag tggatacaac gcatcaaata taataagaac attaacaaat
    30781 cctagatggg ctggcgacac attagaaaag tacccttggc ttaaagataa tttaaaaact
    30841 gttacaaacc gaattataga aattgaatca cgtagaggtg atatgccaac agaagtatct
    30901 catctagcta ataatataca atactttgaa ggtataaatt attttgtaaa tattttggtc
    30961 gctcttggta aagaaacttt ccagagagga tattcatatg gtagcaatta ttcaaaaaaa
    31021 gatgttttaa gttctctact taaaaattgc tatcctacta aagaagatga ttcaaaaaaa
    31081 ttaaaaagtt tacttaaaaa tactgacatt actgagttaa gattgataga atctgctatg
    31141 tatgcccctc aatgggtaaa cataattgaa aaatatttaa actggaaggg tctgaaaagt
    31201 acaatttggt acttccatgc acatataaat gaaggcttct ctgcacaaaa agaatcagag
    31261 gttgctctat tctcccctat ttcaccacgt agtttcattg atggtgcatt tgatgtagat
    31321 tggtttttag attcatataa aactattggg gaagaaagat tcaatatact ttacaagtca
    31381 gctaaatata taactagtgg tggtaactct catagacgtg cacaactcta tgctgatgct
    31441 acacttggta agcttgataa agacatgttg atgaaagaaa ttgaaacaaa tagaaatcaa
    31501 gataaattga gaagtttctc tttacttcct atgaaaaaag gagacctaaa agaagcaact
    31561 ttccgttatg aatttattca aaagtttgct aaagaaagca agcaatttgg tgcacaacgt
    31621 cgtgaaagtg aatcaaaagc ttgtactata gcgctagaaa atctagctag aagtgctgga
    31681 tttggagatg taaatcgtat gatgtgggct cttgagactg aaaaactaaa tgaagttgct
    31741 catctattta aaccaaaaga tattgatggt acttcaattt ggatagatgt tgatgaaaac
    31801 ggaaaagcat ctactaaagt tcaaaaaggc aacaagtctt taaagagtat accaaaagct
    31861 attgctaaac atccatatgt acttgaactt aaatctgtcc aaaaagattt aaagagtcag
    31921 tattctcgtg caaaacagag tttagaacaa gctatgaaaa atgaaatagt atttacagtt
    31981 aaagaaattg ccaatatcta taaaaatcca gttatctgcc ctttgattaa aaatctagta
    32041 tgggtatgtg atgataatat tggatatgtt tataatgata agtctaagtt ctttatttgc
    32101 aatatagaag gtgaaactta taagttgacg gcaaaaaata aattaaaact tgcacatcca
    32161 gttcatttgt ttgaatctgg taagtggagt gagttccaac atgatttatt tagtaagcaa
    32221 attgttcaac catttaagca agtattccgt gagtattata gaataaatag tgatgaatta
    32281 tctgaaaaaa ctatatctcg cagatatgct ggtcaccaaa ttcagccaaa gaaaactgta
    32341 gcacttctta gaaatcagct ttggactgtc gattatgaag agggattaca gaaagtattt
    32401 tataaagaaa atatagtggc aaagatgttt gcacttgcag attggttttc accagcagat
    32461 atagagcctc ctactattga aacagtagaa ttttttgata gaaaaactta taaaagttta
    32521 gaattagctt ctatacctcc aattctattc tcagaagtta tgcgtgatat agaccttgtt
    32581 gtaagtgtcg ctcatgctgg tggtgttgac cctgaaacta gccattctac tgtagagatg
    32641 cgtatagcaa ttgctaaaga acttttatta ttattaaaaa ttaacaatgt caattttgaa
    32701 ggctctcatg ctcttatagc aggtaattta ggtgagtatt ctgtgcacat gggttctggt
    32761 attgtacata aaaaagggat gggtgctttg aatatacttc ctgtacattc tcaaagtaga
    32821 ggtaagatat tccttccatt cgctgatgaa gaccctaaaa ctgctgagat tatgtctaag
    32881 atactattac ttgctgaaga tagtaagatt aaggacccta gtattttaag taatttataa
    32941 gtataaaaac tatccctaaa atatattgga cttcttagtt tataataaaa atattgctag
    33001 aaaagcagat gtaattatat ctataataga tagagagctt aaattaacac tctaaaacta
    33061 tttatagaaa agaggattta aatgttattg attttaggta atttaaattt agacttggat
    33121 ttcgactaca gaataataag agaagaaaat gatgatgtag atatatttat agatattaat
    33181 tatcgtagct tagatataga tacttatggt tctaacttat ttaattctag aatacaattt
    33241 ccatttgtaa gagcattgat tctaaggcta aataagaaca atcaatgtat gacaattcat
    33301 ttgttaagag atatagattt attctctgct tttgccaact t
//
LOCUS       DAEMHW010000021        33299 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEMHW010000021 DAEMHW010000000
VERSION     DAEMHW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.021"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(71..835)
                     /locus_tag="KPK67_000850"
     CDS             complement(71..835)
                     /locus_tag="KPK67_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG2457851.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            1109..1783
                     /gene="fsa"
                     /locus_tag="KPK67_000851"
     CDS             1109..1783
                     /gene="fsa"
                     /locus_tag="KPK67_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG2457852.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1905..3299)
                     /locus_tag="KPK67_000852"
     CDS             complement(1905..3299)
                     /locus_tag="KPK67_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBG2457853.1"
                     /translation="MGIILKPKQFYMTVITTIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVRFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3377..4723)
                     /locus_tag="KPK67_000853"
     CDS             complement(3377..4723)
                     /locus_tag="KPK67_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2457854.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      4973..5075
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5169..6545
                     /locus_tag="KPK67_000854"
     CDS             5169..6545
                     /locus_tag="KPK67_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG2457855.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(6659..7804)
                     /locus_tag="KPK67_000855"
     CDS             complement(6659..7804)
                     /locus_tag="KPK67_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2457856.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            8057..8764
                     /locus_tag="KPK67_000856"
     CDS             8057..8764
                     /locus_tag="KPK67_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2457857.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            8811..10406
                     /locus_tag="KPK67_000857"
     CDS             8811..10406
                     /locus_tag="KPK67_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2457858.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            10509..11591
                     /locus_tag="KPK67_000858"
     CDS             10509..11591
                     /locus_tag="KPK67_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG2457859.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            11740..11901
                     /locus_tag="KPK67_000859"
     CDS             11740..11901
                     /locus_tag="KPK67_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBG2457860.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            12242..13510
                     /locus_tag="KPK67_000860"
     CDS             12242..13510
                     /locus_tag="KPK67_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG2457861.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            13705..14493
                     /locus_tag="KPK67_000861"
     CDS             13705..14493
                     /locus_tag="KPK67_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG2457862.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            14493..14942
                     /locus_tag="KPK67_000862"
     CDS             14493..14942
                     /locus_tag="KPK67_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG2457863.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            14946..17210
                     /locus_tag="KPK67_000863"
     CDS             14946..17210
                     /locus_tag="KPK67_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2457864.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMIDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            17350..17661
                     /locus_tag="KPK67_000864"
     CDS             17350..17661
                     /locus_tag="KPK67_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457865.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            17947..18447
                     /locus_tag="KPK67_000865"
     CDS             17947..18447
                     /locus_tag="KPK67_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBG2457866.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNIFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            18504..19490
                     /locus_tag="KPK67_000866"
     CDS             18504..19490
                     /locus_tag="KPK67_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG2457867.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGYFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            19540..20262
                     /locus_tag="KPK67_000867"
     CDS             19540..20262
                     /locus_tag="KPK67_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBG2457868.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            20291..22225
                     /locus_tag="KPK67_000868"
     CDS             20291..22225
                     /locus_tag="KPK67_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2457869.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNIEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            22218..23486
                     /locus_tag="KPK67_000869"
     CDS             22218..23486
                     /locus_tag="KPK67_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBG2457870.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIHIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMNNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKNDLKKIIFNYKF"
     gene            23715..24755
                     /locus_tag="KPK67_000870"
     CDS             23715..24755
                     /locus_tag="KPK67_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2457871.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            25111..26433
                     /locus_tag="KPK67_000871"
     CDS             25111..26433
                     /locus_tag="KPK67_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG2457872.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            26730..28091
                     /locus_tag="KPK67_000872"
     CDS             26730..28091
                     /locus_tag="KPK67_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG2457873.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            28164..29558
                     /locus_tag="KPK67_000873"
     CDS             28164..29558
                     /locus_tag="KPK67_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2457874.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            29620..30090
                     /locus_tag="KPK67_000874"
     CDS             29620..30090
                     /locus_tag="KPK67_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBG2457875.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            30093..32309
                     /locus_tag="KPK67_000875"
     CDS             30093..32309
                     /locus_tag="KPK67_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2457876.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            32392..33063
                     /locus_tag="KPK67_000876"
     CDS             32392..33063
                     /locus_tag="KPK67_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2457877.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
BASE COUNT        12711 a         3654 c         5760 g        11174 t
ORIGIN      
        1 atgtttttaa gaatagttat caaataaaaa tttatattta taagaaatcc tataaaacat
       61 ataattaaac ttataaaaca tttacattaa attctgataa aatatctact atatcaggag
      121 taggcgtttt ttctgtaatt atagaactaa tatcttctaa atgagcaaac ttgaaatttc
      181 catcaaattt aaacttttta tcttccataa ctataaatac ttttttactt gaatttatta
      241 tagccttctt cgtaagacca tcttcaattt caaaagttgt aagtgaagag ttgaatacat
      301 ctactccaca acttcccata aaagtcttat caaacttata tttcgatata aactctatag
      361 ttggagtacc gacaaaaccg tctaaactga gatttaataa acctccagtg gaaattatat
      421 ttaaattatt tggttttgaa gtaactacat ttagtatatc cagcatattt gtaacaagtg
      481 ttactttttt attactttca gctaacaact tcgcaagcaa gatgttaatt gtagagatat
      541 ctaaaaatat agtttctcta tcttcaatta aattaaatgc tttttctgct attattttct
      601 ttgttggaat attaatttct tttctgtctt ttgtatcctg attctctagt gtttctctag
      661 ctagtacagc tcctccataa attcttctta ttgaagaaat cttttctata ttttttaaat
      721 cttttcttat acaatcttct gtaacattaa atttagaact gagctctttg acagtaactc
      781 taccttcttt ttttaggata tttaatatag cttggattct ttcttctgca aacattttct
      841 acctcttttc gcaatatagt attatagtaa atattataag ataaaataca atatatcaac
      901 tattttaaca tgaaatacaa aattaattta atataaaaag tatgtttttt ctaactatat
      961 atttagaatt tagtaaataa aaaaattata aaactaagaa ggaaaagtaa aacaataata
     1021 aaaaataaaa aacaataagc aaaatgttga aaataatacc aaatcaatat ataataagaa
     1081 taaagaaaga ttaaaggggg atattttaat gcaactaatc ttagatacag gtaatgtgga
     1141 ggagattaag gagttatgta cttgtttacc aatagatggt gtaactacaa atccaagtat
     1201 tgtttcaaaa gaaaaaaaga attttaagca acttataaat gaaattggtg agattatagg
     1261 tgaggatatg cctatacatg ctcaagtgtt aagtacaaag tatgaagaaa tattagaaga
     1321 agcattatat ataagtagtt tgagaaaaaa tatatatgta aaaatacctg taacacaaga
     1381 tggtttaaga gctataaaag accttcacag aaaaggtgta aaaataactg ctactgcaat
     1441 attcactgct catcaaggtt ttttagctgc aaaggctgga gcaaattata ttgcacctta
     1501 tgtaaatagg cttgataata tatctggtga tggagtagct atggtttcag aacttataaa
     1561 aataatagat acttataaaa tggatacaaa agtacttgca gccagtttta aaaatgcaca
     1621 acaagttata gaattaatgc aacatggggt gcatagtgtt acagtaccat atgatatttg
     1681 taaatctatg atgaatcatc cattaactga ttggagtgta gataagttta tagaggattg
     1741 ggagaatact tttggaaaag gaagtaaaac aaataatatt tagtattatg gtaaaagagt
     1801 agttttgtcc aattttacaa gaacttatat aaaaagatga aaatatgaat tttcttttta
     1861 ataatttcgt tattatctta agttaattca tattttcatc ttttttagat ttctagtcct
     1921 ataaatttcc accaagggaa tgttatacat attattataa agtaggttaa aggaatcaaa
     1981 gttaaaccgt atttgaaaat aaccttgcta ttatacagat tttcaccatg accaaccatt
     2041 attgtagcat gatgaaatgg taatatgtac tgtatgctta ctataatgta gacaaataga
     2101 cataaagtaa cactattttc ctttagtata tgtaaacttc caagtaacgg tattactaca
     2161 gatgatgtag tgacagcact tccaagacat atattcaaga ttattgtgag agttatcaaa
     2221 aacaatattg aaataatttc tgaagagttt ggtataaact ttatcaaata atttcccatt
     2281 atatctacaa ctccagagta ctttaacact cctccaattg aaaatgctgc tataaaaaaa
     2341 ataaggacct tccaatttat ttcttttaaa gtactaaatc taactatttt tcttagtaat
     2401 aaaagaagta cacaaattga cattataatt aatgtgttta tgttatgtat gaactgagtt
     2461 aaaaacataa aaaacattat tcctaggcat aaaaatacgt gtttttgctc tttgtttagc
     2521 aaattaaatt ctttttttaa attagaatat gaaaaatgaa gtttattacc ctcaagattt
     2581 tcattagaaa taaattttat tgaatgcaat tcttttttaa agacaaagat aaataaaaaa
     2641 taagttataa aacaagtaat tatagttgga acagacatat aaaaagccca ttgaccccaa
     2701 tttatattac aatttccaag ttctagtaca acataattta gaagagtgtc tccatttaca
     2761 aaaaacattg atgtaaaggt agatgctgta aatatactaa ataaaagtat agattttgaa
     2821 tcttcagtta tctgttgttc ttttaaaaac tccttgtaaa aagcagatac aagaataacc
     2881 cttggaaatg gttgtgggat aaaaaatatt aaaagtatac ctaaaatata cgataaaaga
     2941 atcatttttt taggggtatt tacagtattt aaaattaact tctcagaaat aatttttgcg
     3001 acacctgttt ttgtaactgc attagtaatt atgtatgcaa gaattattat gtaaaaattt
     3061 tctgtaaata aaaactgtaa aataaccttt attggtgcaa tgttaaatat aaacgctaga
     3121 attacaaaaa acaatgcaac aaaagttttt tcaactgagt ctacagccca tatcaagata
     3181 gttaaaaaag ataagcatat agaaaatttt atgttgaaac tgtaattaag agagaagtag
     3241 agaattatgg tagttataac agtcatataa aattgttttg gcttaagaat aatacccatt
     3301 tttatattca cctccataat ttattatatc ataatggagg tgaatacaat gaatgtgaat
     3361 acattttata aaaagattat tcattgattc tatgatttac aatagcttta gttttccaat
     3421 gtcctttctt atagtagata actgtaacaa taagacctaa agtccaacca aaaggaatac
     3481 ccatccaaat tccgtctgtt ccaaaaaatt tagaaagcat ataagaagct ggaagtcttc
     3541 caagccataa agatactatt gagcttataa gtgcaaacat ggaatcacca gcacctttca
     3601 ttgcacttga aagtacaaaa gaagttccaa taaataaaaa gaatggtcct attataaata
     3661 agtaagaaga gccaatttta atgacatctt tatcagtatt gaatagagca attagatttt
     3721 ccctaaatag caaaattaaa gcaataacag taactgctaa aactaaggtc atttttatag
     3781 attctcgaat gcctttttta actctttcat tttctccagc acctatgttt tgagcaacaa
     3841 aagtagaaac agcagaacca agattcataa taggtaagct tataaaattt tctatcctaa
     3901 gaccagcacc aaaagctgcc atagcagatg taccataact attaactaaa aattgaagag
     3961 ccatatttcc aattgaaaac aacatttgtt gaactccact tggaagtcct aatctaagtc
     4021 catctttaaa tattttgtta tcatatacaa ttcctttgat tttaaattta agcacttcat
     4081 gttttttatt taaataaatt atacttatta taaatgatac tgcctgagcc attatagtgg
     4141 caagtgcaac gccctcaaca cccattctta gcactacaat aaaagttaag tctaaaacta
     4201 tatttagaat tgtggctatt atcagaaaat aaagtggagt tactgaatct ccaagtcctc
     4261 taagtattgc acttactgaa ttatatccag cagaaaataa tattccacta aatattatag
     4321 taagatacaa ttttgattga gcaaaaacgg attctggtgt attcataagc ttaagcatgg
     4381 ggccaccaaa tactattcca agaatactta ttaatataga accaataaat aaaagtatat
     4441 atgtagtatc aatagtagct tttacctttt ttaaatcccc tgagccataa aattgtgaca
     4501 ctaaaataga aaatccaagg ctaacaccca taagcgctgc caccaataaa aacattatag
     4561 ggttagctcc agagactgcc gccattgctt cttttccgat aaatctacca actattatac
     4621 tgtcagctgt attgtatagt tgttgaaata aactaccaat aagcataggc atggcaaaaa
     4681 agaatatgga ctttccctca tgacctgtag ttaaatcttt caccttattc ctccaaataa
     4741 aaaaattatt aatttattat agtataatct taacataaac tttatatcca aactataaat
     4801 aaattgtatc aaaatatcat atattttttt aaaatgtaaa gaatataatt ataatttgaa
     4861 tttaataaat caatatttaa tataatcgaa tattaataca aaatcaatac aatatatacg
     4921 tatatattga caaaattaat tatataaatt aaaataatat tcgtaaagaa aaattcatgt
     4981 tttgtactgt ataacatata attttgacaa tatgggtcat aagtttctac cggaataccg
     5041 taaatattct gactatgtat actattattt agttaattaa atatttaatt aactgaatta
     5101 tgaaggatac ggctcagctt atcaactact gtgtcgtttt tttgtaaaat ttaataggag
     5161 gtgttgttat gcaaacaaca aacagtagta ctatgcagaa actttttcca attttgacta
     5221 ataaaaatgt cgacatgaag aaggaaataa ttgcaggagt aactactttt ttaactatgg
     5281 cttatataat agctgtaaat ccaaatattt tatctgagac aggcatgcct gcaggtgcac
     5341 ttgttacagg tacttgttta gcagcagcat ttggatgtat tttaatgggt gtagttgcta
     5401 accttccttt tgctttagca tcaggtatgg gtcttaatgc attttttgca tatacagtag
     5461 ttttacaaat gggtgttcct tgggaagtag ctcttacagc agtatttgta gaaggtatta
     5521 tttttatagt attatcagta agtggagttc gtgaggctgt tgtaaatgcg ataccaaaaa
     5581 atatgaaatt agctgttaca ggtggtatag gaatatttat tgcacttata ggtctagtta
     5641 acagtggaat tgttattgga gaccaagcca ctcttatcaa aatgggtaga tttacaccag
     5701 cagtaataat aacttgtgta ggattaataa ttattgctgt attagataaa aaaagagtta
     5761 aaggttctat actttttgga atagtagtga gttcattact tgcttgggca tttgctttta
     5821 taaatccaga gtatgcacaa aaattaggta tatatttacc tggtggatta tttaaattcg
     5881 aaagtttagc accaatagca ggtaaaatag atttaggata tgtactacat ccaacaaata
     5941 taggtggttt tttagtagta gtttgtacat ttttatttgt tgattttttt gatacagtag
     6001 gtacattagt tggtgtttgt tctaaagcaa acatgttaga tgaaaaaggg aatgttccta
     6061 atgtaggtag agccttactt acagatgcgg ttgcaactac agttggtgca ggacttggag
     6121 tatctacagt tacaacttat gttgagagtt caacaggagt tatcgcaggt ggaagaactg
     6181 gatggacagc aataacagtt ggaattttat tcttagctgc aatgtttttc tctcctgtat
     6241 ttatagcaat tccatcatgt gctacagcac cagctttaat atatgttgga tatttaatgc
     6301 ttggtacagt taaggatata gagtttgata atataactga aggtgttcca gcttttgtta
     6361 ctattgcttg tatggcatta acatatagta taggtgatgg tttaacttta ggtatattaa
     6421 cttatgtttt cgtaaatata ttttataata tatttggagc taaaaaggca gaagacaaaa
     6481 aacatgtttc tgtagtaatg attgttctgg caatattatt tataataaaa ctttggctac
     6541 tataataggt attatttaaa attttaattt aatataaaat aaagggctgt aatttattat
     6601 caattgtgat gataaactac agctcttttg tatttgttgt attattgtat ttattgtatt
     6661 atttaataat gtctattagt tctccatttt gtaaagtttt aggtgtaatt actatattga
     6721 actctttttg caaatctttt ataatatcta attctttttt tgttattatt gagattgtgt
     6781 taccacttcg atttcctcta gcagttcgac cacatctatg caggtattca tttttacttt
     6841 ttggaaaatc caagttaaaa acatgactta catccacaat atctagtcca cgagcagata
     6901 aatcagttgt aattaaaatc tttgctttac caagtttaaa tttatttatt gcattctttc
     6961 tatcttcttt ttccatattt ccaaaaattc ctattgcttt gtaattatga taatttagtt
     7021 ttgatactag gacttctata tttttttcat tatttacaaa tacaatagct ttttttggat
     7081 ttgtagctgc taatgccttt ctaagaaaag taaatttatc ccttatctca cctaataaat
     7141 atgagtgatt gatgttagga tttatttgtg atttttcctt tgtttttatt atttcaaact
     7201 ccttcataag tttatcacaa atttttatag tgctatcagt aagacttgca gagcatccta
     7261 taatttgtct atctcttaac gttgttctta ttatgtcttc tatacaagtt atattttttc
     7321 cattcaatag attgtccact tcatctaaga caatagtttt tatattatgt gattttagct
     7381 tcttttgttt tatcaaatct aaaactcttc cacaagagcc aattactatg tgtggtttta
     7441 ctgcttttat attttttatt tgtttttgta tattgacctc cccaattaaa gctaaagaag
     7501 taacggagag ttctgcattt ttagccaata attcaacctg gtttgtaatc tgcattacta
     7561 attcatgcgt aggtgcaagt ataagagctt gtgtttctct ttttgatgta tcaatttttt
     7621 caaatatagg taaaaggtac gctagtgttt ttcctgttcc agtctgtgaa ttaatcaata
     7681 aatccttatt ttgaattata tttcctataa caagtgactg aacttctgtt ggagaagtta
     7741 tgtcttgttt tttgagtcca tctattagag tggaacttat ttttaattgt tcaaatgtat
     7801 tcatattttg ctccttatat tttaataatt atagtcaact attttttatt atacattgtt
     7861 tattcaaatc gaactataga ttcttttata attatgtgta atttgaaagt aaatatcaat
     7921 tatatttata cctttatatt tctgatacct ttgcatttta tagatttcta aaataaacaa
     7981 ataaaagttg taattgtttg atatttgtta tatattatat ataacaaata tcaaaactat
     8041 aatatggagg gaaattatgt taaaagtaat caatgtatct aaatcatata aaaataaaaa
     8101 agtagtaagt agtgtctcat ttgatgtaaa tgatggagag ctttttgggt ttataggaca
     8161 taatggagca ggtaaaacta ctactattaa agcaattgtt ggaatacacg attttgaaga
     8221 gggagaggta ctaataaatt ctaaatctat aaaaaaacag cctgttgaat gtaaaaagga
     8281 aatggcatat atacctgata atccagattt atacgagtca ttaactggta tacaatacct
     8341 taattttata gctgacatat ttgaagttgc caaagataaa agagaagagt taataaaata
     8401 ttattcaaat aagtttgaac tcaataaagc attaggagat ttaatatctt catattcgca
     8461 tggtatgaaa caaaaattag ctataatttc ggctttgata cacagcccca aaatcttaat
     8521 tcttgatgaa ccttttgtag gtcttgaccc aaaagcttcc tttatattga aggaaattat
     8581 gagggagttt tgtgacaaag gtggttgtat atttttctca acacacgttt tagaagttgc
     8641 tgaaaaaatt tgtgataaga ttgccataat aaaagatgga aatataattg cttatggaac
     8701 tacagaagaa gttaagggaa gtaattcact tgaaagtatc tttatggaat taatagagaa
     8761 ataaaatgtt ttttatacaa tatagtgtct aaagtgagga atgataaaat atgagcaatc
     8821 taagtatatt aataaaaact aatataataa atgaatttaa attaaatgcg ttaaaaaaag
     8881 ctgatggtag agaaaaacaa aaaataatgg gaatgatgct ttcagttgtt ataatagcgg
     8941 gtctattaat tttttatgta acgagcttaa gtctctcttt agttgatatc ttaaagcaaa
     9001 taaatcaact ggaaatgcta ctgatatttg gttttggatt atcaacatta acaactattg
     9061 ttacatcatt atataaaacg tcatcatatc tatttcaagc aaaagattta gaccttttga
     9121 ctagtttacc tataaaagaa tctactgtac tagcaagtaa gatattaatg ttggttggag
     9181 caaactattt attttcttca atatgtatga ttatacctgc agtggtttac tttttaaatt
     9241 ctgatgtaag tataacttat ttcccttatt tgatattgct atttttagct ttaccattat
     9301 taccgatagt tgcttcatca ataatttttc tatttatagg gcagatatca tcaagaataa
     9361 aatacaaaaa tttagtatta atcataggta gcattatatt gatgttgctt tataccctac
     9421 ttatgtcaag acttaattct atgtcagttg aaatgctaaa gaatagtgtg tctatttcag
     9481 agacgattag aaaagtatat cctcctatat attattttgt tgatgctctt aaaacaggaa
     9541 gtatagtttc atttttagca tttatagcca tctcaataat accatttttt atgtttgtaa
     9601 ctctgtttgc aaaaaaattt aaagatataa atgctaagat gagtgaagga tataagtcaa
     9661 aaaattatga atttaagggt caaaaaactt caagaccttc aaaagcttta ttaaaaaaag
     9721 aatttaagag atatacttct tcgtttatat actttttaaa ttcatctgca ggtttgttat
     9781 ttctaatagt aggtacaatt ttaatattat tatttggagc agataaagtg ggtgagttac
     9841 tgaaattgaa tatagatttt aatcttataa aaatacaatt attgggagta ttcttcttta
     9901 taatagtaat gaattgtaca acatactgtt caatttctct tgaaggaaaa aacttatgga
     9961 tactgaaatc ttctccaata gatgaaagag aaatattctg gtcaaaaata ttgatgaatt
    10021 ttttattgaa tgcaccacta tctattttat gtctaatatt agtatctata agattagact
    10081 ttgaaattca gtttattatt gtatcaatct gtttgattgt aagtttttca atgtttgtag
    10141 cattgatggg attattatca aatttattat acccaaacct tgattggaaa aatgaggtgg
    10201 ctgtagtaaa aagaagtgca agtatgatag ttacaatgtt agtatcatta gcttatgtaa
    10261 ttatacttgg aggtatatat gtattacttg gaatttcgtt tttaaatata tatattattt
    10321 tggcaagtat acttactttg atattagatt ttatgatatg gagaattatt tctactaaag
    10381 gagtaagaat atttaaaaat ttataggtgt atttatgtca aataaagact tatctttttt
    10441 agtcttgcaa ataataggtt aagtagtata ctatgtatag tgatatgaaa aaatttagga
    10501 gaaaataaat gagaaaatta tccaaaagag aaaaagattt tattcaatat ggatttttat
    10561 tatttttgat atgtattact acttatttag tattaactac tttagatata aaactaatac
    10621 ctaaaattat aaggctagta aataagactt atatatttct tggcatattg acaattatag
    10681 tatatatgat aattgaatct attgtaactc atttgataat aaatgcagtt caaaaaacaa
    10741 aagtaaaatt tattggtatt aaaatggcta caatgggatt ttactataac ttagttactc
    10801 catttgcatc tgggagtcaa ccaatgcaaa tatatgcact tacaaaatat gatgtaagcc
    10861 ttagtaaatc agtggctata gttactaata aaactgtagt atttcaaagt acagttactg
    10921 tattttgtgg aattttaatt ttgctaaata agtcattact gatggagcaa gtacattctg
    10981 tgaaagtctt gataactttt ggaatgatta tgaatgtaag tatgttgtta ggtggactac
    11041 taatagtatt tagtccaaaa caagtaaaaa tgatagcaga agttttgatt aattttttat
    11101 ctaaatttaa aacatttaaa tttttagatt caaaacgtga tagaataaat cattatattg
    11161 atgattataa ttattctata aaaatattta ttaaaaatgt aagaatctta gttttaagta
    11221 tattacttac tgtgttacaa ttaatcgcat atttcagtat agtttattgt gtgtataaag
    11281 cctcaaattt aagaggagtt tcatatgtac atataatgac attacaggtc tttttatata
    11341 tggctatatc accaattcca acaccaggaa atgttggagc taatgaggtt acatttttta
    11401 ctatgttctc caatataatt ccaaaagaat tattggggta ctctgtactc ttatatagtg
    11461 ggtttgtata ctattttata ttaatagttt caggtttatt tacatttgga acgcattata
    11521 cattaaattc ttggaaaaag gaagataaag taaattctaa tttagataag gttattgaaa
    11581 aattaaatta ataaaataat tagggaaatt ctatatataa atatggtgtt gagtaagcta
    11641 tattatattg agatatttcc cttttttgat taaaattata gaaatttgaa ataataagta
    11701 ataaacagaa aaagtgatat tgttttgaaa ggagaaacta tgaaacaaga taaacaaaat
    11761 gaaaaacaaa gtgagatttc taagataaaa caaagcaatg ataataaact tgaaatagct
    11821 caagaattac atgtaagtaa aaatataggg aatatgcttg gatatagaat agataagaaa
    11881 aaaatgaata gaccaagtta atgaaaatgt tgaaaatggt acattttaaa aaatatatat
    11941 aatgaaatgt tttattgtta aatttaaact tattttaatg gaggaaaaag tcgaaaaata
    12001 tctaaaaaga tatggatata aaaaatatta atatatatat actataaact tgacaaaatc
    12061 aaccttttaa taactagcaa aaaatagcaa aattatataa gcgttattta attgtcaaaa
    12121 atgaaagcta aattgcatgt cctaaaaaaa atgattgata acatttattt gtgtgctata
    12181 attatatagt aaaacaataa gtatattgca taatgtatgc aaatcgggag gagagacgga
    12241 catggaagca tctatacaca caattgccag caataattta ttgataattt ttgctattgt
    12301 aagtataact ggaataattt gtagtaagct tagtgaaata ataaaggttc cagatgtagt
    12361 gttatattta cttgtaggta ttttaatagg accatcattt ttaaaattta tagatattcg
    12421 tggatttcaa atagaaaatc aactaatact tacatttggt tctgcattta ttttatattt
    12481 aggtggaaaa gaaataagtt taaaagtatt gagaaatgtg aagatatctg tattcttatt
    12541 agctacttta ggagttgtga tatctgcttt tataatgcaa caagtaatag gatttacttt
    12601 tgggataagt gcaatgactg ctttactagc aggaactata atagcatcaa cagacccagc
    12661 cactcttgta cctatattta atcaggtaaa gataaaggat agggtaaagc aaactgtaat
    12721 aagtgaatca gcttttaatg atgcaacagg agctatacta acatcagcag tgttggctgt
    12781 aattttgtca aataaatttt cattaggtga aaatatttat gaactgggga caatggttat
    12841 tgtcggagtt ttagttggtt taattacagg aatactgctt ttgaaattag ttaatgataa
    12901 accttatgga atatttaaag attttgcacc aataatatct gttatttcag ttgtagttgc
    12961 atatgaatta tctacaaaat taggtggaag tggatatatg tcttgcttta ttgtaggtat
    13021 aataactggt aacaagaaaa actttaaaat ttggttaagt caaaaatctt atgatgcaga
    13081 tttttatgtt gctgaaactt taggtactat ttgtcgtatg gcaatattta taatattggg
    13141 tagccaagtt aatttggctc agttaagtaa atattttatg ccatctgtaa ttacagtatt
    13201 agtattaatg tttatagcaa gaccactttg cgttttagtt tgtacacttg tagacaggga
    13261 agcaaaatgg agtaaaagtg aaatattatt tatgatgtgg gtaagggaaa caggcgtaat
    13321 accagcagca ctttgtggta ttatttcagc aatgaaagtt cctggatacg aagtgatttc
    13381 atctgtagta ttcatgacta tactaattac attagtaata caaggaagta ctacaaaatt
    13441 agtagctaaa aaacttggat tattagaaga agaagtgata aatataagtg aaaaagtatc
    13501 aactttttaa agttttgctt taaaatagta cctgttaatg gctattttaa tattgtctaa
    13561 tgatgattat tgaaatagtc atttttttat gtataagtaa tttacagaat ttacatatta
    13621 gacatattta ttatcaaaat ttgttgaagc tatgatataa ttagttgtat ttaaaataag
    13681 aatagcaaat aagggaggaa atttatgttt acggttatgg atattgtaca gccagataca
    13741 gtagaagaag cgtactcaat tttaaataaa agaaaaacta accaagtaat tggtggaagt
    13801 gcttttttaa gaatggggaa aaaaagaata ggaacaggaa tagaattatc taaattaaat
    13861 ttagattata taaaagagta tgaagattac gttgagattg gttctatgac tacttttaga
    13921 acattagaaa caagttccat aataaaaaat aattttggaa gaataataga agattcagtt
    13981 aaaaatataa taggagttca gtttagaaat gtagtaacag ttggtgcaac agtattttct
    14041 aaatatggtt tttctgactt gatagtagca ttactttcat tagatacgga agtagaatta
    14101 tataatattg ggagaattag tcttgaagag tttctaaata gagattatga gaaagattta
    14161 cttattaaga tatacataaa gaagacaaat aagaatgctt cttataaatc acttagaaat
    14221 gcaaagagtg attatccaat tctaaatgta tctgtatcaa aacatatgga acaatttaag
    14281 ttatgtgtag gagcaagacc gcagaaagct acgattgcaa aacaagctag tgaatttcta
    14341 tctaacaatg aaatcaatga aattaatata gataaagcag tagaaatagc atcagaagaa
    14401 ctaacttttg gttctaacat gagagcatct agggagtata gaaaagctat gagtaaagta
    14461 cttttaaaaa gagctataat ggaggttatt taatgttagt aaagattaat ataaatggga
    14521 agaaaaaaag tgttgatata gaaccagaag aatatctggt tgatacttta agaaaagttg
    14581 gtaatttaag tgtgaaaaga ggttgtgaca caggctgttg tggtttatgt acagttttaa
    14641 ttgacaagaa acctacatta tcctgtgcaa cactgacatt gagagcttta gataaagaaa
    14701 taactacaat tgaaggttta gaaaatgaag ttaaagaatt tagtgaagtt ttggtaaaag
    14761 aaggagctga gcaatgtggt ttctgttctc ctggctttat acttactgta cttgcaatga
    14821 aggatgagct tgaaaatcct acagatgatg aaataaagca ttatcttaca ggaaatctat
    14881 gtagatgtac agggtatatg ggacagttaa gagctatcaa aacttatctg gaggtaaaat
    14941 agaacatgaa tatagttgga aaaggaatca agaaaataga tggaatgagt atcacaacag
    15001 gaaagcctgt atatacagat gatttatcag caaaagatgc tttggtggta aagatactta
    15061 gaagtcctca tgcttatgca aaaataaaag atatagatac atcaaaggca gaaatgattg
    15121 atggtgttga atgtgtttta acatataaag atgtaccaaa atctcgattt acacttgctg
    15181 gtcaaacata tccagagcca tctccatatg atagattaat tcttgaagat gttgtaagat
    15241 atgtaggtga cgaagtagct atagtggcag ctatagatga aaaaactgca attaaagcaa
    15301 tgaatcttat caaagtaaag tatgaagtac ttacacctgt tttggatttt gaagaggcaa
    15361 taggaaataa aactatggta catgatgaag atagacattg taactttgat ataggtatgc
    15421 aaagagaaaa aaatatagta tcccatcatg attatacaaa aggtgatgta gaaaaagtat
    15481 taagtaattg tgatgtagtg attgaagaaa cttattatac tcaagctcaa gcacatgcaa
    15541 tgatggagac gtatagggca tacaattatt tagaccatat gggaagattg gttgtagtaa
    15601 gttcaactca aataccattt catgtaaaaa gacatttatc tcgtgcacta gaaataccag
    15661 ctagcaaaat tagggttata aaacctagaa ttggtggcgg atttggtggt aaacaaactg
    15721 catgtgttga gattttttct gcaattgtta ctttaaaaac agggaaacca gctaaaataa
    15781 tttatgatag aaaagaaact tttaattgtt caacaagtag acatgcaatg agattaaaag
    15841 ttagactagg tgctacaaat gatggcataa taaaggcaat agatataaaa gcactttctg
    15901 atacaggagc atatggagag catgcttcta caactattgg tttggttgga gaaaaaactt
    15961 taccaatgta taataaagtt gaagcaacaa gatttattgc agatgtggta tatactaata
    16021 agatgccagc aggagcactt agagggtatg gagcatcaca aggatgtttt gctgttgaat
    16081 caactataaa tatgcttgct aataaattaa aaatggaccc aacagattta aggttaaaaa
    16141 atgtggtaac agaaggtgaa actacatttg catatggtaa aactttaaat agtgtcgatt
    16201 taataaaatg tataaataaa ggcaaagaac ttattggatg gaatgaaaaa tatccttgta
    16261 aacatcttga taatggaaaa attagaagtg tgggtatggc aattactatg caaggttctg
    16321 gtatacaagg tcttgataag tcttcagctg aaataaaatt gaatgactgt ggagattata
    16381 ctcttatgat aggttctact gatatgggta caggaagtga tacgatttta gctcaaatgg
    16441 ctgctgaggt tcttgataca agcattgaaa atataacagt tatatcagct gatactgact
    16501 tagttccata tgacccaggt tcatatgctt caagtactac ttatgtaaca ggtatggcag
    16561 tagttaaggc agctcaagac ttaagagaaa aaataatttc tgctggagca actaaactag
    16621 gtgttgagaa ggatgaagta gaatttgatg gtataaaatt aactagtaaa aaaggtagca
    16681 ctttattatt cgatatagca gttgattcta caattggtcc agatactaat caattaatag
    16741 gtcttgcttc tcatggtagc ccagtatctc ctccaccttt tatagcaggt tttgcagaaa
    16801 ctgaaattga cacagaaact ggaaaagttg atttagtaaa ttatgtagct gtagtagatt
    16861 gtggaacggt tataaataaa aatctagcta agatacaagt tgaaggtgga attgttcaag
    16921 ggattggaat ggcattattt gaagaagtta gatacacaga aaaaggaagc atggacacaa
    16981 atacatttat gcaatacaag attccagcta gatgtgatat tggaaatgtt aaagtagatt
    17041 ttgttgaaac ttatgaacca actggaccat ttggtgctaa atctgttgga gaagttgtta
    17101 taaatacacc tttaccagca atacaagaag ctgtgtttaa tggaattggt gtaagaataa
    17161 atacactccc tattacacct gaaaaagttt ttatggaaat gaataaataa ttatttatga
    17221 aaatttagac tagttttaca tgttaaacaa ccagttatgt catacctgtt gaaagatatt
    17281 taataagtag ttattataat attatgaatt ttacttataa aatacaaaat taaataaggg
    17341 gatgatgttt tggctggtaa tctaaataat atgagagcag taaataattt tagaggagat
    17401 aagaacattt tagaatgttt agtcagcttt gagggtcgtt caataagtca gagaaaagta
    17461 agggtatttt ttaaagaaaa acaaaatcaa atagaaattg attttgcaga agaggaaatt
    17521 tctaaattgg ttgaaaatgt tgttttaaat acatcatatc aagaaatgtt atatgatgaa
    17581 atagagaaac aactggaaat tgattgtata ggtacttgga tgatattatc taaattaaaa
    17641 gatggtagta gagttcactg attttaaaat aaattattag agcctaattt taaggctctt
    17701 ttttgctgtt tttaattaac aatagaaaac ttatctttag tttgtatata attttaatat
    17761 catatattct atatatataa aattgaattt gtatgtatta tattgtataa tgtaaaatga
    17821 attggaaaaa tagttcgata aaataatttg tttttataaa attactgttt aagtttatag
    17881 attttgtata aaataaagtt atgttattaa tttttttaga aataaaactt ttactaggag
    17941 acagatatga gaaaaaatag tattaatata gcactaactg cagcaattac agcaatctat
    18001 gcagttttaa cattatcatt gggatttata agttatggac caatacaatt tagggtagca
    18061 gagattatga tgcttttggc atttttagat aaaggatata tagtaggtct tacattaggt
    18121 tgttttttag caaatgtaat aggaccatat ggtgtacctg atataatttt tggaacatta
    18181 gccacattta taagtgcaag tatgatttat attactagaa agttaggtaa acaaaataaa
    18241 agtactttag ttatagcttc tttatggcca actgttataa attcgattgt ggttggtcta
    18301 atgttaaata tattctttgg gcttccatta attctatcta tgatacaggt aggttttgga
    18361 gaatttgtag tagtgacaat tgtaggagta ccatttttct gttttttaat gaaaaaatat
    18421 aaaaatgtca ttaatattaa attttaaata gtgtatagta atttaattaa ttgagagtat
    18481 tattagtgaa aagaggtgtt tatttgtcga aggtaaaaag attgactgat gtttggatat
    18541 atattaatga taagcaaaag tttacaataa aagagttgtc agaggaattt aatctttcag
    18601 ctaaaacaat acaacgatat ttaaaagaat taaataaaat gggacttcca attcaagcag
    18661 aacaaggtag aaatggaggt tatagggtgc taaataatag ctatatacct cctgttatat
    18721 ttacagaaaa agaagttatg attatactat ttgcacttaa gtcacttcag atttataatt
    18781 ataagttaat ggaaatagaa attaattcta taatgagaaa agtaagttat gaacgtaaaa
    18841 gcagtataaa agtaggtata gaaaatatta aaagatacat tggctttgta gctaatgata
    18901 ctaatgatgg atattttaag agtagtaatg taactgagtt ttttagagct tcttcagaat
    18961 ctctaatttt aaatattaag tataagtgtg gtaatcagat tttggaaaaa aacgtatgcc
    19021 ctataggtat atatttaaat aagggagcat ggttttcacc agcatatgat tgtaaaagtg
    19081 ataaaataat attagttcat atagataata taattgatat aaaaagaaca ggagaaagta
    19141 aaaaaataca tataaacttg gaagaatggt tacatgaagt atataacaat ttatatggaa
    19201 atagttcaac tactaaaaaa tttggtgaaa ttatttatct aaatgtatta ttaactaaag
    19261 aaggtgtttc taaaataaag gacagttctt ggaatttaga tatggagcta aatgaagatg
    19321 gtagtggaat aattagtaca ttcataaagg ttgaagatat agattgttat gcttcgattt
    19381 tattttttat tggaaataat gctaaggtta tagagccaaa atttttgaat aagctacttt
    19441 atgataaggc caatgagtta agatattttt atgaagaaaa tatggtataa tatttagaaa
    19501 tgttgaaatt aaataattat taaggagata tacgttataa tggaaaattt taataagaat
    19561 gctaaaataa ttggtattgg agctgaaggt attaatataa taaatgaagt agaagaaaaa
    19621 ataaaagcaa atatggatat agaaaaaata aacataaatc aagagattga aaaagaatat
    19681 gttagaagtc ttttagatgg tgtagatata ttatttttga tatattcatc tgaagataaa
    19741 catattagag atattataaa agctgtttca tatatgtcaa acgaaagaag agttctatct
    19801 ataggaatgg attgttcaga gaaagaaaat aaagaagatt tagagcttgg gagagagttt
    19861 aaaatcaata atgatagtat atttaaattt gttgatttaa tgaatatcat ggtagagtct
    19921 atttctgatt cttgcatgat aaatatagat attactgatt taaaagaagc tattgtagga
    19981 gataaaggaa ttaagtattc ttttgaagag tttgaagata caaagtcata tagcgagata
    20041 gctgacattt tatttgacag gatggaatat attggtgagg aatttataag taagaaagga
    20101 attgtatttg ttgaaggaag tccagaattt tctataatgg aattgaatga tttaataagt
    20161 aatattcaga gcaaggtaga agaaagctat gaagtaatat tttctttata tataaaggaa
    20221 aatttaaatg gaaatattag agtaggctta ttgtataatt agtatatttt agatattaag
    20281 gagagtgtat atgtctgaaa atataagagg tacagagtat tggttaatgg aagaattttt
    20341 ttatcaggaa acagacacac cacagatttt tagagaagaa agcataattg cacttggatt
    20401 tgatataaaa ataaatttaa aaaaagtatt cagtatgggg aaaaaatcag aaaagtatat
    20461 taatgagaat tgtaatgata aagattttat aaaaaagttt atgtcgatta aaaaaggaga
    20521 ttatttttta tataaagcat atcaaaatat agaagaaaag gttaaatgta atgtgtctat
    20581 tggcatagtt gaagaagatt ttgaaaatgg atatgaatta agtcatattt tagggcatac
    20641 actaccagta aaatggatgc ataattttga tacagtttca aattgtaaag tatatacaag
    20701 tgaattttat aaattaaata ataatgatat tagctcatat ttagaaatta ttaagtcaca
    20761 agataagaaa ttattgagaa agagaataga agaaagtgaa aataataaat ggtatttaaa
    20821 aaactattat tataaaaatg tagataagtg tgtagatgat gaagaattat ttattcagcc
    20881 cattaatcta gaacaattag aaagcctaaa aaatataaag acagatgatt atattttatt
    20941 gtataatagc gagaaagtaa tttgtgaaaa tgaccataca atgaagagtc tatttggcgt
    21001 atgtcaagtt atggaagggt tcgataaaga gaaagtaagt gctttggagg gtggatattt
    21061 ttttcctgtt agatggatag agtatgaaaa ggagaatata gacggaaatg catgggttat
    21121 atttgaaaat aatagtatag ttgaaaagta tgtaaaaaga tttactaata aaatgccatt
    21181 aaatactata ctttatggac caccaggaac tggtaaaaca tacaatacaa aacagatggt
    21241 ttacaatcta gtaagtgaaa ccagtctaag aaacagatac tatacaggtt acttagatta
    21301 taaaaacaat atagaatttt gtacatttca tcaatcatat ggatatgagg aatttataga
    21361 gggtcttaag tctgatggtg aaggcaattt taagccagaa gatgggatat taaaggatat
    21421 atctatagaa gcatgttata atgcattgaa atttgaaaaa aagatgagct ttgagttgga
    21481 aaaagatagc ttgactttag aaactataaa agacaggaaa aagaaattag tattagaaaa
    21541 tatagaatat agtgagagtt ttaattttga agaatcagaa aactatgttt taataataga
    21601 tgaaattaat agagggaatg tatctaaaat atttggtgag ctaataacat tattggaaga
    21661 tgataaaaga cttactaaat caaatcaaac tattgtcaaa ctaccatatt caaaggaaaa
    21721 attttctcta cctccaaatt tgtatatagt tggaactatg aacacatcag ataaatcaat
    21781 agcactttta gatatagctt taagaagaag gtttaatttt gaagaaatta tgccagatta
    21841 tgatttattg tctattgtag atggtataga tattagaaaa atgctatata caatcaataa
    21901 aaggatagag tttttatatg atagagacca tgttattgga caagcttatc tacttgctgt
    21961 agaaaatatt aatgatatag tagtagtatt tagaagtaaa ataataccac ttcttcaaga
    22021 gtatttttat tatgattcag aaaaagtagg tttgatttta ggaggaattg gaagaaatga
    22081 aaaagataaa tacattgttt ataaagaaaa acttatagct tctgagttat ttaaaaataa
    22141 taatgcctca ataatggaga gtaaagttaa ctattgtata aaacaaaaca tatctagtga
    22201 ggagctaaaa aatatatatg agtaaaaata tatatgttaa ggaaacctat gaatggataa
    22261 gggtaggtaa tggtgaaaat gagcttacag aaattgaata tgaaaaattg cttaaatatc
    22321 tagagaacaa taatgatgta ttaaaaagta atataataga tattaaatac aaaaaactta
    22381 gatttataaa ctatgtaggc atcatatgtt ttgaaaatgt aatacttgaa atacttccta
    22441 aattgtcatt aagtgataat cttgtaaaag atagagagat actattacag atgctttcta
    22501 tatgtaataa aattccaatt acaatgaatg aaaaaataag attgagtttg aaaaattata
    22561 atctcttaaa tttttttgtt atgtatttca ttgagagtat gcaaacacag atgaaaaaag
    22621 gtatttattt tgaatatata aataaaatag agaacttaaa tgttatgagg ggtaaaatat
    22681 tattaagtac atatgcaaaa gaaaaaggca tttctccaat gaaaataaga tgtgaatatg
    22741 atgagtatag tgaaaataat ttcttgaatc aagttcttaa aaaagcatgt atatctattt
    22801 tatgtagaat aaatgacaat tcaattcaag gcaagattaa aaaaatttta agctattttc
    22861 aaaatgtaga tttaattcat atagatagaa aaaaactgtt ggattataaa ttttataaaa
    22921 acaatgatag atttaaagat tgttatttac tagctagact tctactttta aatttgtcta
    22981 tgaataatag tcaaaataat caagaggcat tttctatatt atttgaaata aatactttgt
    23041 atgaagaata tataggaatt ttaatcaaaa gtatatggga caattcattt agagaaacat
    23101 atatacaaga taagagtaaa tttcttttga aaaatgaaca aacaggtaag aaaaacttta
    23161 atttaagacc ggatatagtt ttatatgact taaaaaatga atatgaaatt ataattgaca
    23221 ctaaatggaa agctattgaa gtagattcaa atgtttttta taggtcaagt gatatatacc
    23281 agatgtatgc atatataaca gcatatgaaa atgcaaaaag atgtatatta ttatatcctt
    23341 gtattcaaaa agataagaat tatagttcct ggaaattatc agaatcattt aagggtaagt
    23401 ttatagaagc aaaaactgtg aggttagatg atataaaaaa cacaaaaaat gatttaaaaa
    23461 agataatttt taattataaa ttttaaaaat tatttccata taaaatatgg cattgtaatt
    23521 gcttatttaa tagaggattt ttgtaaaatg aagaaagtat atctatcaaa ttaaaactaa
    23581 gtagatatac tttttattaa gttttgtttt aaaataatat tattaaatta taactaagct
    23641 tttctgttat gatataatta aatagataat gtgaatgatt attatataga atacttattt
    23701 ggagttgata ttttatggac aaacacagtg attcagtaat gtatggtgga atggaagtat
    23761 ataatatttg gggaacaaca acagtagact ccataattgg agagtctgat gtaatgcgtg
    23821 ctcttaagaa tagaatttgt aaaattggaa atagtgattc tactgtagct ataactggag
    23881 aaagtggcac tggaaaagaa ttaatagcaa gagccattca tctcgcagga aacagaggga
    23941 ataaaacatt cgttgctata aactgtgctg ctataccaga acctttacta gaaagtgaac
    24001 tttttggata tacaaaagga gcttttagtg gagcagattc aaaaggtaaa attggtaagt
    24061 ttgaattggc caatggaggg gttgtatttt tagatgagat aggagatatg ccaattcaac
    24121 tacaatcaaa gttattgagg gtattacagg aaaagaagtt tacgagaata ggttcaaatg
    24181 aactaattga tatagatgtt agaatcatat cagctactaa tagagattta tttgagttgg
    24241 ttaaggaaaa taaatttaga gaagacttat actatagatt gaatgtaata cctttagaag
    24301 tgcctccact tagagaaaga ggagacgata tacaaatatt aataaacaat tttatagaca
    24361 aatattcgag aaagatgaag aaaaacgtgt accatataga gccagatgtg gaacaaatgt
    24421 tgcttaagta tccttggcca ggaaatatta gagagttgga aaatactata gaactggcat
    24481 taaaccttct tgaggaagat ggaataatac ataaaggaat aataaataga aagatacttg
    24541 aatattttaa gttaaataat ataaatataa agttacttga ggaaagtgac tatgagataa
    24601 gtatggaaca agacatactt acacttgaag agtttgaaag agcctatata aagaaagtgt
    24661 taaagtttta cggaaatgat actaagtcta aaaaactggc tgccaaaaaa cttggaatat
    24721 cactagctac attgtacaga aaaatagatg tataataatt aggtttttta ttctcaattc
    24781 taaaatttga aattataaaa ttgataaaaa acacttggaa aactattttc taagtgtttt
    24841 tttgtgtaat agcattttat tatccaaaaa taaaaaaaat ttaaaagaat ttaaaaaaat
    24901 aaatgcttta ataaattatc aaatactaac aaatataata aaaattatat atgtaaaaca
    24961 attatgttaa aaaattggca ttataattgc ttcatattaa gttgagatga caaaataaaa
    25021 aaagaaagga tgttgtgtga gataataata actgtcatta aaaaatagtt gggagtgata
    25081 accttgagta aatatcaaaa gaatgtttct atgtttgata ttgatggaag gccaccaatt
    25141 ttaaagtgtt tgccactatc aatgcaacat atactagcaa tgatagttgg aactgttaca
    25201 gtacctatag tcgtaggagg agctgtgggt gttagcccta cacaaataac acttttagtg
    25261 caatatgcat taataatagc tggtatatca acacttatac aactatatcc aataggaaat
    25321 attggttcaa gattgcctat aatttttgga gttggtttta cttatgtacc aacacttgta
    25381 gcagtcggag gaaattatgg attagcaagc attttgggtg cacaattaat agggggagtg
    25441 gctacagtat taatagggat attcataaaa aagataagaa aattttttcc gaatatagtt
    25501 gcaggaacag tggtttttac aattggatta tctttgtatc ctatagcaat aaattatatg
    25561 gcaggtggtg taaatagtcc tacttatgcc tctatgcaaa actgggcagt agcggctata
    25621 acattagtgg tagttatagg tcttagtcaa tttgcaaaag gatatgcaaa attggcagca
    25681 atatttttgg gaattatagt tggatattgt gtatctttag ttctgggtat aataaatttt
    25741 gaccctatta gagaggcaac ttggtttgcg atacctaaac cgcttgaatt tgggatggaa
    25801 tttgatatgc ctgtaataat ctcagtagta attgtaagta taatcaatgc tgttcaagca
    25861 attggagatt tatcagctac tacaatggga ggcatggata ggaatatcac agataaggaa
    25921 ttatcaagtg gagtaatagg aagtggaatt tgtactatga taggagcttt gtttggaggt
    25981 ttaccaccat catcatatag ccaaaacgtg gggatggtag ctatgaacaa agtaataagt
    26041 agatttgtgc ttggaatagc tggtatattt atgttattgt cggggtttgt gcctaaattt
    26101 ggtgcattaa tgactactat accatattct gttttaggag gagctacaat atctatattt
    26161 agtatgataa caatgacagg tataaagtta attattacag aagagttgtc atcaagaaat
    26221 attaccattg ttggtttggc aattgcctta ggcatgggaa taacatcagt accagcagcc
    26281 caagaggcat ttccttcatt tgtaaagtta gtatttggag aatctccaat agtaattgca
    26341 acattggttg cattcgtttt aaatctaata ataccaaata aaagtctaga ggatgaggct
    26401 aaagaaagag ctgttatgga agagactaat taagatataa taatttaaaa gaattgttta
    26461 aattatttag catgagattg ttagattatt tttgatagct attcatttta ataaaaaatt
    26521 taacaaataa atatatacca ggcaattaat gttcatttaa gttgaaaatc ggggagggaa
    26581 tatttagaaa tttgttaatt ttttataaaa atagctcagt ttagctgtgt ttatcggttg
    26641 agcaataaat tcatggtcat aaaaatattt atagaaaata attataaaaa tatttagtca
    26701 agatatttat gtatgggagg atttatttta tgaaaacacg taatacttca aaatatgatg
    26761 tcgatgggat accaccacta aaggaatcaa tacctttggc cttacaacat ctgttagcaa
    26821 tgatagtagg aaatatggta cctgcgatat taatagcaaa cgtggtagga cttaaccagg
    26881 ggcaagcaac aatgttgatt cagggttcta tgttagcagc aggtttagca acgtttttac
    26941 agttatatcc aataccttta tttaaaggat ttaagttagg ttcaagatta cctgtaatga
    27001 tgggaatgag ttatgtattt ttaggagctt gtctttcagt agcagctaaa gatggtttag
    27061 cagctctatt tggagcacag attgctgctg gggtaatcgt attctttgta ggatttggag
    27121 ttaagaaaat tagacatata ttcactccaa tagtttctgg aactataata gcatgtatgg
    27181 gacttggact attcgcaaca gctataaaga atttagctgg aggagaaggc acacaaacat
    27241 ttggaagccc aataaacttc acagtaggag ttatagttgc ttttgtaata ataatgataa
    27301 ataaatacgg taaaggttta gtaaaaaatt cttcaatatt aataggaata ttagtaggtt
    27361 atgctatatc tttagtttta ggattggttg atttctcagc cgttcaagga gcagctatag
    27421 tatctttacc aacaccagca gcttttggat tagaatttag accagagtta atagtaatgt
    27481 ttactattat ttacataata ggtatagctg atatgatggg tgcctgtaca atagctacag
    27541 taggagcaat ggatagagag gtaactgacg aagaattagc atcagttgtt ttaggaaatt
    27601 ctataacttc tataatttca tcactatttg cagctcttcc aactggagta tttagccaaa
    27661 atacagtaat tgtatctatg aacaaagtta caagtagatt tgtaatagca ctaggggcac
    27721 ttgtattatt attagcaggg atatctccag cattgggtgc aataatgaca actataccat
    27781 cttgtgttgt tggtggagca actttagtag ttttctcatc aatagcaatg tctggtttct
    27841 ctattatgag tatggatgga ttcactgaag aaaacaatct tatagcaggt gtatctatag
    27901 caacaagtat gggtcttaca actgctccac aagttttaga ccaattccca gaaacaattg
    27961 gaactgtcct tggagggtca agtatagttt ctggagcaat aatagctata ttacttcaaa
    28021 cattatttaa attaaagtca cgtaaaagtg ctgaaaacgt tacttcaaat cttgaggaaa
    28081 atattggata aatgtattag atataatatg agaaaaccat atggtaaaat gttgtaatga
    28141 aagtattcaa agggggattt aagatgattg atatactaat aaaaaatgca ataattgtta
    28201 cagttaataa agacagagat gttatatttg atggagcaat agctattcaa ggtgacagaa
    28261 tcttggacat aggaaataca gttgatattg agcctaagta tcttgatgct aaaaaagtta
    28321 tagatgctaa tggaaaagta atattccctg gctttataaa tactcataat cacctgttcc
    28381 aagtgttatt aaaaggttta ggtgatgata tggcacttca tgagtggtta aatacaatga
    28441 tgtttccatc agcaaaattc ttaacagaac aagatactta tgatgctgca atgcttggat
    28501 gcatggaagg tttaaaaagt ggtataacta caatggttga ttatatgcat actcacaata
    28561 gaccaggact tacagatgga atagtaaagg catataaaga tttaggtata agaggggttg
    28621 taggtagagg ttgtatagat ttaggtattc acaaagagct tatagaagat gtggaaacgg
    28681 ttgaaaaaga tttgagaagg gtattcgaaa aatatcataa ttcagaaaat ggaagaataa
    28741 aaatctgtgt agctccttct tcaatgtggg ctatatctga agaaatgggt aagatgcttt
    28801 ggaatatagt taaagaatat gattcatatt ttacagttca tatatctgaa acagaatttg
    28861 caagaacagc aacaaaggat atacatggag aaatagatat aaaattactt gaaaaatggg
    28921 gtatagtagg tccagaagtt gtagcagtac attgtgtatg tataactgat gaagatatgg
    28981 aaatgcttaa aaagtatgac ataaaagttt ctcataatgt agcaagtaat atgtatcttg
    29041 cttctggtgt tgcacctgtg ccagaaatgt taaaaaaagg tataaatgtg agtttaggtc
    29101 ttgacggtgc agcaagtaat aacgcacaag atatggtaga gcttatgaaa ctaacagctt
    29161 tacaacacaa agtaaataat tgtgaccctc tggcaatttc tgcagagaaa gttcttgaaa
    29221 tggctacaat tgagggagca agaactctta gaatggaaga tgaaataggt tctttggaga
    29281 ttgggaaaaa agctgaccta gtaatattta actcaatgct ttctccaaaa tcaatacctc
    29341 ttcacaatcc tgtatcaact ttggtatact cagctagtat gcacaatgta gagagtgttt
    29401 tagttgatgg aaatgtaata cttgaaaatg gaaaagtaac tacaattgaa agtgaagaac
    29461 aagtgttgct taatgcacaa catgcagcag aagaattatg taagagagct aatgtaacta
    29521 atagaatgga aggacataag tggaatagct tatactaata ttaaaataag attgtaataa
    29581 tattctatta caacattagt ttatctgtag gaggagaaaa tggaaaaaat aaaattaaat
    29641 attaatgatt acatgtatga aatagaagtt gaaaagaatg aaactctttt gcatgtactt
    29701 cgtgaaaaat taaatctaac tggagcgaaa caaggttgta atagtggcag ttgtggagca
    29761 tgtaaagttt tgatagatgg agttgacatg aaatcttgta aacttcttgc aaaaaactgc
    29821 gaaggtaaag ttatatacac tatagaaagt tttgctaatg gagaaaatct tcatccaata
    29881 caagaagctt ttatagaagt tggagctgta caatgtggat attgtactcc tggtatgatt
    29941 ataacaacac aagctctttt aaatagaaat gcatctccta atgaagatga aattagagaa
    30001 gcttttaaag aaaatctctg tagatgcaca ggatatgtaa agattataga agctgtaaaa
    30061 ttatctgcag ctagattagg gggtagataa ttatggaaaa ggtaatagga aaatcatacc
    30121 ctctaaaaga tgcagcttta aaagttactg gtcaaatgaa atacgtggct gatatgaaac
    30181 cacaaaatgt actttacgct aagatgcttt taagccctgt agcacatgca aaaataaaaa
    30241 gtatagatac aagtgaagct gaaaaagtag taggtgttag agctatagct acttatctca
    30301 atacatcaca aagaaaatac aatggttcat taagatttta tgagcacaaa atacctgaaa
    30361 atgaagttat attttcagat acagttagat ttgttggtga ccgtgttgca gcagtagccg
    30421 cagagactcc agaaatagca gagaaagctg taaaacttat aaaagttgaa tatgaagaat
    30481 tgccagctgt atttactata caagatgcaa taaaagaaga tgctatctgc atacatggag
    30541 attcaaatat agtttctcaa aacaacataa atgggggaga tgtagaaaaa ggcttcaagg
    30601 aatgtgacta tatatttgaa gatgaatact ctactccagc aatacatcat agtgcaatag
    30661 agactcatag tgtaattgca cactatgatt ataatgataa attaactgta catacacctt
    30721 gccaaaatac atttggattt agaataatac tatctcaaat ttttgggatg tcattaaata
    30781 aagtaagagt tataagacct gctattggag gctcatttgg tggaaaatta gaacttacaa
    30841 ttgaaccagt agcagccgca ttatcaaaaa tgacaagaag accagttaaa atggttctta
    30901 caagaaaaga atctatgata tcgacaagaa ctcgtcatgg gtcttttgta aaattaaaaa
    30961 ctggtgtaaa taaagatggt gaagtaatag cacaagatat aaaaatatat acaaatacag
    31021 gagcatatgc atcaagtgct ttaaatgtta taggagcact tagtcataaa gtatttaaag
    31081 tttataaaat accaaatata aagtttactg ggatgcctgt atatacaaat acaccaatag
    31141 caggagctat gagaggctat gggtcacctc aaatatttat ggctcaacaa gctcaatttg
    31201 cgaaaatagc aaaggaaata ggtatggatt tagtcgattt tcaaaataaa aatgcagtag
    31261 agccagatga tgtcgatata atcttccacg gttctcttgg aaatcctaga gtattggatt
    31321 gtatagagca aggtaagaag atgtttaagt gggatgaaaa gaaaaaactt ccaaaagaag
    31381 aaggaaaata tttaagaggt ataggtatgg caataggtgc acatggtaat ggagtttttg
    31441 gtgcacatag agatgttgtt acattgactc ttaaattaaa tgaagatggt acgttgacac
    31501 tactaacagg agctcatgat atgggtaatg gtgttgtaac tatgcaaaca atgatggtag
    31561 ctgaagtttt aggaataaca cctgataaag ttgatacatt tgaaactgat acggatgcat
    31621 gtacattcaa ccttggtgac tatgcaagta gaggggtatt tgtagaaggt ggaggagcta
    31681 aaaagacagc agagaaattg aaaaatctta tattagaaga agcagaaaaa ttacttgaaa
    31741 catcaaaaga agatttatat ctagacaatg gatgtgtaat tagtaaactt gatgaaaatg
    31801 taaaagcatc actttctgat gtcgcagtat atagtcaaag taaatcatta agagagttaa
    31861 ctgttgttga agattattct tcaccagctg gacgtacttc ttatggtgta cattttgctg
    31921 aagttttagt agataaagaa actaaggata taaaagtagt tgattttgta gctgtacatg
    31981 atgttggtag agttataaat cctctaaatt tagaaggaca attagaaggt gggattcata
    32041 tgggtctagg atacgcattg agtgaagaat tggcatttga tgaacaaggt agacctaaag
    32101 ctactgattt taaaacatat aaattattta gaactgtaga tatgccaaaa tgtcaagttg
    32161 catttgtaga tggttatgaa aaagctgggc cttttggtgg taagagtatt ggagaatgtg
    32221 ccgttgtacc agttgcgcca gctgttgcaa atgcagtata taatgcaact gatgtagaga
    32281 tacattcatt accaataaaa ttaaaatagt agaaattaaa gcagaaataa caatttattg
    32341 tatttgtaaa tagataataa aaatagattt atttggtgag gagatattat tatggcaaag
    32401 atattaggta tttgtggaag tccacgtaaa ggagcaacag aatatgcagt gttagaagct
    32461 ttgaaagaag cagaaactat acctggtata gaaactgagt attggtcagt tagagggaaa
    32521 aaaataagtc cttgtgtaca ttgtgatgca tgtataagaa aagaaactat gtgtattata
    32581 aaagatgata tccaagaatt agagcaaaaa atattggagg cagatggaat tatagtaggt
    32641 tctccagttt atgatatgaa tataactgct caacttacag cagtatttaa tagacttcgt
    32701 ccaatgtatt tagttcatcc aggaaaactt caaaataagg ttggtgcagc tataactact
    32761 ggtggaacta gatatggtgg ccaagaattg acaaaacttc ctataataaa tttcttccta
    32821 atgcatgaaa tgttagtgtc tggaggttta ggtggatgct atataggtgg tacaatatgg
    32881 agtaaagatg gaaaagcaaa aggtgcccaa gaggatgaga caggtatgga cacagtaaaa
    32941 agacttggaa aaggtgcagc agaggcagtt atggtttcta agtttggtct tgaaaaatgg
    33001 aatgtcgtaa aagaagaatt agatgttaaa aaagatgaaa aatcacctct tagagaccac
    33061 taaagattta ttatattatt gaatatatac aaatagtgat gtaatagatt attgcatcac
    33121 tattttatgt tttaatttag attttataaa ttttatatta aaaaatatag aaacaaagtg
    33181 ttttgagatt tatttttaaa ttttaaaaat gagaattaaa aagtgagaag atagaaaagg
    33241 ttttttagtt attaaattct acatacattg tatgaactaa agatagaaaa gaaaaaata
//
LOCUS       DAEMHW010000022        33215 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEMHW010000022 DAEMHW010000000
VERSION     DAEMHW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33215
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.022"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            206..772
                     /locus_tag="KPK67_000877"
     CDS             206..772
                     /locus_tag="KPK67_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2457878.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(991..1977)
                     /locus_tag="KPK67_000878"
     CDS             complement(991..1977)
                     /locus_tag="KPK67_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBG2457879.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(2007..2702)
                     /gene="pxpB"
                     /locus_tag="KPK67_000879"
     CDS             complement(2007..2702)
                     /gene="pxpB"
                     /locus_tag="KPK67_000879"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBG2457880.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLKSIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(2722..3942)
                     /locus_tag="KPK67_000880"
     CDS             complement(2722..3942)
                     /locus_tag="KPK67_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBG2457881.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(3971..4762)
                     /locus_tag="KPK67_000881"
     CDS             complement(3971..4762)
                     /locus_tag="KPK67_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBG2457882.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKMGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            5350..5979
                     /locus_tag="KPK67_000882"
     CDS             5350..5979
                     /locus_tag="KPK67_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2457883.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(6093..7607)
                     /gene="glpK"
                     /locus_tag="KPK67_000883"
     CDS             complement(6093..7607)
                     /gene="glpK"
                     /locus_tag="KPK67_000883"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG2457884.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRTLSWEKEDELDI"
     gene            7851..8393
                     /locus_tag="KPK67_000884"
     CDS             7851..8393
                     /locus_tag="KPK67_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2457885.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            8407..10899
                     /locus_tag="KPK67_000885"
     CDS             8407..10899
                     /locus_tag="KPK67_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2457886.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKVVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLKKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSINQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            11320..11718
                     /locus_tag="KPK67_000886"
     CDS             11320..11718
                     /locus_tag="KPK67_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2457887.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(11935..12144)
                     /locus_tag="KPK67_000887"
     CDS             complement(11935..12144)
                     /locus_tag="KPK67_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457888.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(12176..12385)
                     /locus_tag="KPK67_000888"
     CDS             complement(12176..12385)
                     /locus_tag="KPK67_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2457889.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            12611..13030
                     /locus_tag="KPK67_000889"
     CDS             12611..13030
                     /locus_tag="KPK67_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2457890.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPFNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            13258..13713
                     /locus_tag="KPK67_000890"
     CDS             13258..13713
                     /locus_tag="KPK67_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457891.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(13942..14202)
                     /locus_tag="KPK67_000891"
     CDS             complement(13942..14202)
                     /locus_tag="KPK67_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457892.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(14465..14818)
                     /locus_tag="KPK67_000892"
     CDS             complement(14465..14818)
                     /locus_tag="KPK67_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457893.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLSQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(14835..20156)
                     /locus_tag="KPK67_000893"
     CDS             complement(14835..20156)
                     /locus_tag="KPK67_000893"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457894.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGHGNTMLIDNKGILKVAGNNDIYQLGIANYSENIDSSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSDYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTMVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNIFGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDVFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSGYVNGLSNKSASQYT
                     EIGVSNVTDAYSSYNATFIVADEKKVYATGINTNYLLGVNPNDGVNINLTLINNWFYV
                     NPSGASYSTNSCTNDITKINNIIIYEYMAILCTNYGSFLTGYHGTLWTKPSDSTYRVQ
                     QQGITHAGYISNYIYNYYPTRCTQSSYSTNFAYLYYGESTSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFAKINIDNIKSISVNRFTDNSKHAFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNVFTKINVENVKYVTVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYLCNDVSIIVKNDNTCYVCGFVNGYFGFTESSISTFTKINIENVKS
                     VVTAGSEATFFITNDNMIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSITNNNNTNSVEDVKDVFVTANNTLYIANNNNLISSGRDT
                     YGISDEYYMDIPVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTFT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIITTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPVPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(20173..21156)
                     /locus_tag="KPK67_000894"
     CDS             complement(20173..21156)
                     /locus_tag="KPK67_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG2457895.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(21157..21855)
                     /locus_tag="KPK67_000895"
     CDS             complement(21157..21855)
                     /locus_tag="KPK67_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG2457896.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMISREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(21852..22904)
                     /locus_tag="KPK67_000896"
     CDS             complement(21852..22904)
                     /locus_tag="KPK67_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG2457897.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTVI
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVNNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTNVNFSEVENQ"
     gene            complement(22897..23325)
                     /locus_tag="KPK67_000897"
     CDS             complement(22897..23325)
                     /locus_tag="KPK67_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG2457898.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(23325..23651)
                     /locus_tag="KPK67_000898"
     CDS             complement(23325..23651)
                     /locus_tag="KPK67_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG2457899.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(23667..25199)
                     /locus_tag="KPK67_000899"
     CDS             complement(23667..25199)
                     /locus_tag="KPK67_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG2457900.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISKKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGTEFSAIFTA
                     YYPGPGIEGGDTDCREKKLNPSKKTCAAPMVGAYEKPYYTKEFLSKHPLFKYGDEVSI
                     VTGVSGRDGLYKVNDNGSAIIIEKDGTYHIDVLVKNAEEMKRFGKRKGKIIIGGYSGN
                     ASNKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKG
                     KKVEQKNLQAGDLVFFHNPVSHVVLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARR
                     VL"
     gene            complement(25481..25903)
                     /locus_tag="KPK67_000900"
     CDS             complement(25481..25903)
                     /locus_tag="KPK67_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457901.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(25903..28356)
                     /locus_tag="KPK67_000901"
     CDS             complement(25903..28356)
                     /locus_tag="KPK67_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457902.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIIIKAKDMTKRVISSILEKLKRVEKRPYQGSINLKDMVSSAMARILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSSKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(28356..28523)
                     /locus_tag="KPK67_000902"
     CDS             complement(28356..28523)
                     /locus_tag="KPK67_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457903.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(28550..28996)
                     /locus_tag="KPK67_000903"
     CDS             complement(28550..28996)
                     /locus_tag="KPK67_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2457904.1"
                     /translation="MSENGLSKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(29122..29550)
                     /locus_tag="KPK67_000904"
     CDS             complement(29122..29550)
                     /locus_tag="KPK67_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG2457905.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(29564..30628)
                     /locus_tag="KPK67_000905"
     CDS             complement(29564..30628)
                     /locus_tag="KPK67_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG2457906.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(30633..31076)
                     /locus_tag="KPK67_000906"
     CDS             complement(30633..31076)
                     /locus_tag="KPK67_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457907.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTLTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(31325..31765)
                     /locus_tag="KPK67_000907"
     CDS             complement(31325..31765)
                     /locus_tag="KPK67_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457908.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(31870..32067)
                     /locus_tag="KPK67_000908"
     CDS             complement(31870..32067)
                     /locus_tag="KPK67_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2457909.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGIINDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            32236..32556
                     /locus_tag="KPK67_000909"
     CDS             32236..32556
                     /locus_tag="KPK67_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG2457910.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            32638..33144
                     /locus_tag="KPK67_000910"
     CDS             32638..33144
                     /locus_tag="KPK67_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457911.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
BASE COUNT        10898 a         5633 c         3714 g        12970 t
ORIGIN      
        1 atactcttct attaaatttt tttattatta ttttctttat attaatttaa caataattca
       61 ttttcataga cttttatttg gatttaatta aattttaatt gtcaaaatct actatgtatt
      121 cttattgcca ctacttctat gttgtgctat aattactttg ttttaagcga atattttata
      181 ttaattttat tatttggagg ttcttatgct aggtgaaaag atgagaaata taagaaaaag
      241 caagaaaaaa acattaagtg atgtttccaa attgacagat ttatctataa gttatatctc
      301 tcaaatagaa agagatgcta ttgaaccatc cttgtcttca ttgagaaaaa tcgctgaagt
      361 tttagacact cctttatata tgtttatgga tgacaataat actgatgatt tagtcattag
      421 aaaagaagat agagttatga tgaaatttcc aaagagtgaa atgttttacg agatagtatc
      481 tccaatgcct acaactgagt ttacaccttc aattttattt gtagagtttg aacttaaacc
      541 agaaagtgag gatgccaaag attatatata tcatgcatca gaagaaatta tggtggtaac
      601 gagtggtgtg gttgatattt gtatgggtga aaaggtagtt agattaaatc caggtgactc
      661 aacatttata aaagctaatg taccacataa aatcatcaat ccaagtaaag atacaatagc
      721 tcgtggatat ggagttatat cacctccaat ttggccaata aaatctaaat aaactcaata
      781 taacatacct actttttgta gataatacaa taaatttaaa tatcgatttt attttaaaat
      841 tgaagtattt atttaaattt aaaatattta ctcaaactaa aaaaatagct atttgactaa
      901 tctaaaccat cttaattatg gtaaatagta aatagctatt tgtaagttat attataaatt
      961 ataataaaaa ttgtatttgt taaaattaaa ttaaactgct atatcattta atacttttgc
     1021 ctttacaact cttatatttt tcatactctt gtagatatct tgaatcttat cttcttgctc
     1081 tctcaatagt ctatgtgctt catatatatc tacaagttca aattttacta catcatttgg
     1141 ctttgcttgt gctaacttat acaaatctac acttataacg ttccctatct tagtatagcc
     1201 accactagtc tgtctatctg ataacattat aatcggtttt ccatgaccag gtacttgtat
     1261 agctccataa gaaataccat cagatataat atctgctcca ttaaggtgct ttatactctc
     1321 tccatctagt ctacaaccca ttctatctat ttcattcgta actttgtatt tatttgataa
     1381 aaatttctct acttcaccgt tagaaaaagc atcaaattga ggacctttta caattcttag
     1441 tacgatttct gaactataag ttggaatata ttttgaactt aattttttat ttattgtaaa
     1501 atctttttcc tgaataaaag tatttatata atctcctttt ttaagtgctc taccttcaaa
     1561 tccaccaatt ttagctcttg tataagttga cttacttccc attgcaagtg gaacatctat
     1621 tccaccagct attgacacat aggctctagc acctgatttc acacctctaa atgataatac
     1681 atcacctgga tttatttgat atgacctcca gttttgaatt tcttttccat ttattttagc
     1741 ccctaaatct cccccagtta ctgctatttg cgtatattca tcaaaggtaa ttgtaggacc
     1801 taacattaaa acttctaaag aagcctcatc ttcagaattt cctactaata tatttcctac
     1861 tctatgtgca tactcatcca ttgctccaca tccagataca ccatattgac catagccaac
     1921 tctacctctg tcttgtatta atgtttggaa acctcctaat tcaactttga atcccatata
     1981 taccactccc tgtactataa cgatatttaa atacttaagt tatttttttc gatttctata
     2041 aattcatcta atgttattgg aacaaatttt ataaagtctc cagctctaag caaaatctgt
     2101 tcttctctat tttcatcata taattttaaa ggtgtcctac caattatttg ccatccacca
     2161 ggactatcaa taggatatac accagtctgg gaacctgcta ttccaacact cccaccataa
     2221 attttagtcc ttggcacttc taatctaggt gtagctattc tttcatccat accacctaga
     2281 tatggaaatc caggagtaaa ccctagcata tatatcaagt actctctact tgtgtgtatt
     2341 tttataactt catctacagt gagattattg tagcttgcaa cgtttttaat atctatacca
     2401 aaaacctcat cataacatac tggaatctca tgtattttag gttttggtaa tactatactt
     2461 ttcaaattat cttctatttt ttttaaattc tcaatcaaac tatcaaaatc tatttctaat
     2521 ggattataac ttatcataag tgacctatat gttggtacca tttctgttac aataccttta
     2581 agattcgata tttctattgc tctcataaat gaaattactt ttttgtttat atcttctgat
     2641 atctcatttc caaattcagc aaccactgcc ttatcccctg atatcaagta tctagtttcc
     2701 ataaatgtca cctcttctaa attaagcaaa taaagcacct aggctgctaa gagaagttat
     2761 tcctgtatat gcagatatta aaactactat taatccaaat attaaaagcc atgtaggatg
     2821 cttgtaatct ccaacaatat cttttctttt agatgctata agcataattc ctaatgtgat
     2881 tggaagtatc aatccattta aagctcctgc taaaattaat aacgtagctg gttttcctat
     2941 aaatgccatt ataagtgttg atattgctat aaatgctatt ataaaatatt tttcatattt
     3001 atctataaaa ggatttagtg tctttaagaa agaaactgat gtataagcag ctcctataac
     3061 tgatgtaatt gcagcagacc ataatacaag tccaaagaat ttatatccta ttgctcctgc
     3121 actaaattta aaagctgaag cagctggatt ttctgggtca agttgaagac cttttgaaac
     3181 cactgccagt attgctaaaa ataaaagaac tctcatcatt gtggcaacta atatacccaa
     3241 aagagaagat ttagttattt cttttatatt ttcttctcct gttattccac cgtctattaa
     3301 tctatgtcct ccagcaaaag ttatataacc tccaacactt cctccaagta aagttattat
     3361 tgggaatatc agtccttttg gattttctgg ttttaccatt cttgatactg cctctccaac
     3421 aggaggatga tttgatattg ctacatatcc aactataatt atcattccta atgctaaaaa
     3481 tttagttatc ttatcgacta gagaatttga attttttgac ataaatacaa atatagctat
     3541 taatccactt aatacagttc ctaatgtcat attcatatta aacattacat tcataccaag
     3601 tgcagctcca ccaacatttc ctatattaaa agctaatcct ccaagagcta ctaaaaaagc
     3661 tactaaatag cctaatccag gtattatttt atttgctata tcctgacctc taagtcctga
     3721 tacaccaatt actctccata catttacttg agcccctata aaaaggattg ttgtaattaa
     3781 aattacaaat gaaaaattcg gtccaaaatc ttgggtaaat tgtgcagtct gagttaaaaa
     3841 ccctggtcca attgctgatg ttgccattat aaatgcagcc cctataagtg ctccgacatc
     3901 ttttttattt ttagttttat ctgtaatatt ttttgtactc atattttatc tccccctcat
     3961 gttatcagtc ttatactatg ttctctgacg aacaaacttc tatgttttct aatggacaaa
     4021 cttctatacc ttctaattca aaacgctttc ttatttcttt gacaaactct attgcttttg
     4081 gattgtcccc atgtacacat atagaatctg ctttgatatg tataacttca cctgttaaag
     4141 tttctacagt tccttctttt accatcctaa caaccctatc tattgcatgt tttgtatcat
     4201 gtataactgc tccttctaca tttcttggaa ccaaaaaacc attattatca taagctctat
     4261 ctgcaaatac ttcatttgca aacctaagtc caatttcttt agcagaattt atcatttcac
     4321 tattatatag ccccaacagt ataatatctt tatcgacttc atatatagct tcacaaatcc
     4381 ccatagctaa ctctttatct ttagctgcca tattgtataa tgctccatgt ggttttacat
     4441 gttgtatatt acatccattt gaacttgcaa atgccattaa agcacccaat tgatattttg
     4501 tataatcttt agcttcctgc tttgttattt tcatctcacg tcttccaaat cctattaaat
     4561 ctaggaaacc tggatgtgcg cccattttca ctccattttt tttagctagc tgtactgttt
     4621 tttccatatg actagggtca cctgcatgaa acccacaagc tatattggct gatgaaatat
     4681 attttaaaac ttcttcatct aaaccaatct tatacgttcc aaaactttct cctaaatcac
     4741 tatttaaatc tactttatac aatttatgac ccctcctcgc taacattttg atagaataag
     4801 tagttttaat ttttaaattt tgtttatttt atatattgca tgaaatattt tatcacttgt
     4861 attcaatttt atttttgcaa ctattctatt gcatttaatt aatataattt taccatttaa
     4921 ggaatatttt tgcaattatt ttaattgaac attcatttgt atttcttttt caaaaaatct
     4981 ttttaccatt ttttagttaa taattaatta ttttcactta aaataagggc tttaaccttc
     5041 atttttaaag tatttttcat ttttaatcac tttatatgca agacagaatc atttttcttc
     5101 attcatttgt ttctttcatc tatataatta tcattctttg taagtaaaat atctttatat
     5161 ttttataatt aattctttat atagttttaa gtataataaa atcatgtata ctaaatgcat
     5221 gttaaaatat tattttaaaa catgcacaat atttgtacat attgtagaca tataaaccta
     5281 taaattatac aatagtattg ttaagattat atattttttt aacataaatt ttgtattggg
     5341 ggaaaaacaa tggataaaaa ctatacgatg ccaatatacc aaaaaatagc gttggatatt
     5401 gcaaataaaa tatacacggg tgaaatacaa gaggattcag tactatttgg acgctctgtt
     5461 ttggctggta aatacaatgt atctccagaa acgataagaa gagctgtcaa aattttagag
     5521 gacattggcg ttgttaaaag cataaagggt aaaggtgtta ttgtactatc tccagacaaa
     5581 gcttcttctt ttataaaaaa atatagagat attacaaata tatcatcata taaatcaaca
     5641 ctttataatt taatagatac taaatctaat ttagaaaatg aaatattaga tactataaat
     5701 aaaattttag actattctaa cagacttgaa ataatcaatc ctttagtgcc cgttcagttc
     5761 accataaatt ctaattgcaa atatattggt caaacagctg cacaaactaa attttggcaa
     5821 aatacaggtg ctacaatagt tgcaattaaa agaggtgaag aacttataat atctcctgga
     5881 ccatatattg aatttttaga aggagacatt ctcttagtag ttggtgacca acacatttac
     5941 aattctatac caatgttttt atatgaaaac gaaaaataaa ttatataatt tagtatgatt
     6001 atataaaaat gcaaaattaa caacaaaaaa agagcttcat attttacata aagctctttt
     6061 taaattcaaa taaatttaac tttgattaat ttttaaatat ccaattcatc ttctttttcc
     6121 cagcttaaag ttctcgatac agcttttttc catcctttat ataatttaca tctcttctct
     6181 tcactcatat ttggattaaa ctctttttct actgaccact ttgaagtaat ttcatttctt
     6241 ccaatataga atccaacaga caaccctgct aaataagcag ctcctaaagc tgtagtttct
     6301 actattttgg gtctatctat atctacattt aatatatctg attgaaattg cattaaaaaa
     6361 ttattattac ttgctccacc atcaactttt aatgatttta gttttaattt tgaatcgttt
     6421 tgcattgctt ctaaaacatc ctttgtctga taagcaattg attccaatgt ggctcttact
     6481 atatgttctt tttttgctcc tcttgtaagt cctaaaattg aacccctagc atacatatcc
     6541 caatatggag ctccaagacc tgtaaatgct ggcactacat atacaccatt agtatcctct
     6601 accctatttg catacatttc actatctttt gcagattcaa ttatctttaa ttcatctcta
     6661 agccattgaa cagatgctcc tccaataaat atacttcctt ctaatgcata tgttactttt
     6721 ccatcaactc cccaagcaat tgttgtaagt aacccatgct tagatttaac tatgttactg
     6781 cctgtattca taagcaagaa acaacccgtt ccataagtat tttttgcagt accttcttca
     6841 acacaatttt gaccaaataa tgctgcttgt tggtctccag cacatcccgc aataggtatt
     6901 ttagctcctg ataacatgcg ctcatctgta tgaccataaa catagctaga aggttttact
     6961 aatggcaaca tgcttattgg tatgtccaat tctcgtaata tatcctcatc ccattctaaa
     7021 tcatttatat tgtacatcat tgttctagat gcatttgagt aatctgttac atgtactttt
     7081 ccacgagtta aattccatat taaccatgta tctatagttc caaataataa ctctccttgt
     7141 tctgcttttt ccctagctcc ttctacatta tctaatatcc attttacttt tgttgctgaa
     7201 aagtatgcat ctattaaaag acctgttttt tcttttattg attcttccaa tcctttattt
     7261 ttaagttcat cacatatttc tgacgttctc ctacactgcc atactattgc gttataaata
     7321 ggtttaccag tatacctact ccaaacaacc gtagtctctc tttgattagt tatccctatt
     7381 gaagcaattt cttctggtct tatacctgct gtttctataa cttctctcat taccccactt
     7441 tgtgaccccc atatttccat agggtcatgc tctacccatc cagcttttgg ataaatttga
     7501 ttgaactctt tttgtgcaat tttcactatt tcgccttttt tattaaatag tatagctctt
     7561 gaactggttg ttccttggtc taatgccatt acatacttct tttccatcac catattctcc
     7621 ttatcatata atatttaatt tattaaaaag aaaaatttaa tatcacatat ttcaataaat
     7681 aatcatactt aaatacaaat ttaatataat agatatttag taagaagaca cttattttct
     7741 atcatatcta tataataata ttatacatta tagcttacat attgattaat aaattttaaa
     7801 ggagtataat aaacagtgtt cataataatt ttaataaaag aggtggtaat atgccaaaga
     7861 ttttagaaaa tgtaaaagaa gatatattaa aagtgtctcg ggatatgatt ttagaagaag
     7921 attactcgaa tattagcata aggaaaatag ctcaaaggtg tggtataagt gcaggtacag
     7981 tgtataatta ctttaattca aaacaagaaa taatagaata tatcacaaaa tctgaatggg
     8041 atttattgat aaggcgaatt gaatactcca ataaaaacac tgaagattac ataaaaaaac
     8101 taaacattat ttttactgaa ataagaaatt ttataaacaa agttcataat atccaataca
     8161 atgacttttt aaacactttt gaaacagaga gattttttga aatgaaaaaa cacaaagacg
     8221 actttcatga acagctttca aataaagtat atgaagcttt agaaaaagaa agttttttta
     8281 ataatgacaa attagtatgc aatatcataa taaaaatgtt tttttcatat tcaacatatc
     8341 ttcatataga atttgaagat ttaaaaccct atattgaaaa attaatacat taaggtggtg
     8401 tttaccttga atcataatac tagtattaag aacaaggaca gtaatccaaa aatattttta
     8461 gtacttacac tttatctatt aggtatattt atgggagcta ttgatactgg aatagtatca
     8521 ccagcaagaa cgattataca aaatagcctt ggagtaaatg aaaaaacggg aatttggatg
     8581 attaccatat acacattaac atacgcttct gtaattccta tctctggaaa attagcagat
     8641 aaaataggaa gaaaatatgt gtatctagtt agtatatttc tatttggtag tggttcatta
     8701 atatgtggat tatcatcact attttccaat ttctatattt tattaattgg tcgtgttatt
     8761 caagcaattg gtggtggtgg aatcatgcca attgctacag cagaatttgg aactaccttc
     8821 ccagaaaata aaagaggcat ggctttaggt ttagttgggg caacatatgg tattgccaat
     8881 attttaggtt caagcattgg tagcacaatt ctaagtatat ttggaactca aaattggaag
     8941 tggctatttt ttgtaaacct tccaatttgt ttaatcatac ttattggtgg agttttctgt
     9001 attaaaaaca ataagtctga atctaaagaa aaaatagata agcttggtac attgatgttg
     9061 atatgtataa tcgtatcact attatatggt cttatgaata tagacttttt taattttaaa
     9121 aattctatac aagatatatc agtttatcca tatttattaa catttattgt acttatacct
     9181 attttcatat ttattgaaaa tagagctaaa gaccctattt taagctttga atatttttta
     9241 aatccgagaa tattgatagt acttatttta tctttaattg ttggtattgg aatgatgggt
     9301 atggtattcg ttccacagta tgctgaaaat gcattaaaga taaatgctgg ttctggtgga
     9361 tattttgtag ctatattggg attatttgca ggtattgctg ctcctttaag cggaagactt
     9421 attgacaagt ttggggcaaa aaaaatacta ttactaggat tttctgtatc tatgataggc
     9481 tctctttacc tagttttaat tgctctaaaa accaatactg tatttagtgt atgtgtaagt
     9541 ttaatattta tgggattagg aatgggattt actatgggaa ctcctctaaa ctatatgatg
     9601 ttatcaaata caagattaga agaatctaat tcagcacttg caacattatc cttgatacgc
     9661 tcaattggta cttcaatttc tccagctata atgattggat ttatagcaca tgcaggatta
     9721 tctgtgcaag ataatattat ggacttagtt ggaaaaccct caactcctaa agtagttcaa
     9781 cttgaagaac ttgaaaacat gatagatgac ttaaaatcag accctgaaat ggcaaaaaat
     9841 ttaaaaaatg tctctatccc aaatatgaat gatacttcaa atataaatat ggacatgaca
     9901 ggtggcaaac ttccaaaatc cttacttaat aaagttcagt cagcagatac aaccaacatt
     9961 accaatataa caaaagaaat atcaacaaca atgtttgatg aaaaagttcc atctataatt
    10021 actaaaatac agaaaaatgt acaaaaagga atagatggaa cacaaagtgg aataaacggt
    10081 attgaagatg gtgaaaataa actaaatagt ggtattaaag gagttcaaac tggtattgac
    10141 aatatggtaa aagctagagc tggtattaat caaggaatag atggtgtaaa aaaaggtatt
    10201 gctggtgttg acaaaggtat aaaaggtatg gaacaaggtc ttaaaaaaca agatgaggcc
    10261 atcaaggaac ttacaactgc ttataataaa atacctacta cagaaaaaac tcctactgat
    10321 aacgaaaata actcaaatga acaaaaccag agtaatacca gtaattctga atcagacgac
    10381 actaagaatg attctacaga aaataagaat aacaattcca gcgagaataa accaaatgta
    10441 aatgaacaaa aagaaagttt aaatgctcaa atacagcaac taaaaaagac aagagatgac
    10501 ttaaatacaa agttacaaaa aaataaaact caaaagaacg aattaagtaa aaaactaaga
    10561 tcaatggaat ctcaaaagaa agagctacaa agcaagttaa ataattctat aaaccaaaag
    10621 aaagacatgg aaaaaagcct tgatacaatg caacagcaaa aaaaatcact ccaagcagtt
    10681 ttagcaaaga cacaagaggt aaaaactgaa ataccaaagg cttttgataa atcaaaattg
    10741 gattatataa attccataga aaataataga acaaaaatag aaaacacact acaatctact
    10801 ctaaactcag gatttaaaca gatgtatata acagtattct gtgtaaatct actagcattt
    10861 ataatattat tgttctataa agaaaataaa actagataaa agagtgttat agtattatat
    10921 tgtaaattta tcttattctt aaatatttta tcctaaagaa taaattcata aaatttttgt
    10981 aattgattaa tataaattac aatataatac tatttttctc ttaatctttt tctcttttac
    11041 agacaatcaa aaagcaatct tagtttatcg aaataaattt atacaatcta ttcatacacc
    11101 cctttacatt tttataacaa ttaaacctat tgttatatat acaaatcaaa ataaaccata
    11161 tattttcaac tataatatgt taaaatcata ttatgagcat attaatttaa gcaaaatgtc
    11221 atttagactt taatttagta gttttatatc ataaatttat atatttttaa ttttaaatgt
    11281 catttagaca ttactcataa aaagaaagga gtgtattttg tggatacttt aggaaaaaga
    11341 attgcttatt tgagaaactc caaaaaactt actcagcgca aacttatgga tattttaaaa
    11401 tttgaaaact taggcaagta tgaaacagga gatagaaaac ctaattgtga tatactaatg
    11461 tcaatagctg attattttaa tgttacgact gactggcttc tctatggtaa agagaaggta
    11521 aatgtcaata gtagtgtaaa agaagataaa gaagattact tacatgttac taatgatgaa
    11581 atgacgatat taaatctata cagacaacta aacgaacgtg ataaaataaa aatagaagga
    11641 atacttgaac ttaagatatc tgaatataaa gacttaaaaa agcattcctc taataataat
    11701 gaagataaaa tggtttaaac atacataatt tgatacgatt ttacatcatc tattaacctt
    11761 ctttttttgt agtatattac taaaatatgg atattggatt atttaaaaac aagtatagtc
    11821 taatttcaag cacatttata atatttagat tatcttatcc atattttagt ttaagtactt
    11881 tgaaaaatat aatacatctc tatgtataac ttatattcct aagaaatgca ttactcatgt
    11941 ttttaattct tccaacatct ttctatacac aattgtacat ccttcataat tcccctcttt
    12001 aagaaggatt gaacatgtac tagaatattt agttaaagta tttaatcttt ccatatttaa
    12061 gctttttata ttactgagtg ctgtaataaa atcatcaatt gctctacaac actctataac
    12121 cccatctgaa tccaatttac acacaagaat attcacctac tttatatact atttattaat
    12181 catcttctga atattctata ttaagtaatt gataaattga tgatttatat tttttacctg
    12241 ttcttcttcc atttaaaata gctgacaaat agttttcatt aacacctatt ttcttcgcta
    12301 attccctttg agtcatattt aattcaataa gtctttgctt gacttgcact cccaaaaaag
    12361 gtatccattt ttttcgagca tccatttatc aacttccttt ctaagtgatt tatatttact
    12421 tttatattat tttagcttac acaccccctc tatttagtat atatttttaa tatgtacaca
    12481 tattaagtat ataaatttat ttaatactaa ataagatttt ttgattactt tgtgctaaaa
    12541 ttaggatgta aattcgtatc attaagttaa ttataaagtc tttttgacat taattgaatg
    12601 gagagtgttt atggatactt taggagaaag aattgtttat ttgagaaaag caaaaaacct
    12661 taaacaatat gaactagaag aaatgttggg ctgtgacaac ttaagtaaat ttgagagaaa
    12721 cattagaaaa ccaaattatg aaatattaaa atctatagct gagatattta atgtttcagt
    12781 agactggcta ttaaatggag ataatctatc acataaatca gatttaattt gcgattcctc
    12841 ctcaaactat ccatttaata gcataaactc taatgaaata aagcttttaa acaactttag
    12901 aaagttaaat gattatgata aagccaaaat agaaggtatg attgaattaa aacttcatga
    12961 atatgaaaaa gagaaggatt taggaaaaat tgagtataac aaaaataaag atgagaaaat
    13021 agataaataa tttttatgtt gcttttgata cgattttaca tcatttcttt gttctaatat
    13081 ttttatttca tttattttct tttttataat tttatgataa aataattagt atattttgct
    13141 ttacttgaat gtaattattt atacaaaagt caaacataat ataaatgtag taaattttta
    13201 gtttcaagta atatctcgtt attataacta aatatttatt aaaattagga ggataaaatg
    13261 ggcttaagag acaaattcgc gcaatcattt gctagatcaa aaactatgag tggccctgag
    13321 aaaaaggcta atgaaataat gggtaaattg cttcttaaga aagctatatt acctatcgtt
    13381 ttaatgttcg tgataataat cgctggagct atgcttaaaa tcaattcttg ggttacactt
    13441 ggcataaatt tagttattgc tgttggtgct ttcttctaca taagaaattc tagtaaaaag
    13501 tatcagaact ttaaacctta tgttggcaat ttgattagtt tagagaaaaa aggtaaaaaa
    13561 gagtacgttg ctattataaa acaaggtaag ttaccagtta agttacaaat agcttatggt
    13621 ggtgaagacc ttgaacatgt taagaaaaat caaatggttc agataagtta taatccagat
    13681 gctaaaatag ctattttggt taatagacaa taatttaata aaaaaaggag ttgtcttaaa
    13741 attgattttt tctaatcaag tttaagctca actccttttt gaatttttac atgttatttt
    13801 tatatattat ttttattact tttttaatta atatttttaa atattaaatc cttctcaata
    13861 aaatagtgca ctatctacaa tactacttta tcttttccta ataatatata atctggtgct
    13921 ggtgatactg ctactttcat gttatacacc gttttgtaac atacctttta tatcagtaac
    13981 atcttcctta atttcctcta catctgtttt cattattccc atttgaacaa gtatattttt
    14041 gtttatttct tgttgttgtg tagataattc tataaaattt tctacagttt ttttatacat
    14101 gtctctatct tcttttttct cctgcatagt atttttaaat aatagagcac atagtatacc
    14161 tattgcccct agactactca attcggttat taatttatcc attatagcct cctattgtcc
    14221 aaatttttta taattatagc tttaattttt gttgtattct tacatcatgc aatattatat
    14281 tttcagaatt atcaatttaa tcttctgtac tttaaattgt aggtgtaatt tatagattga
    14341 ataaataata aatatatata caatttctaa tataacaaaa aataagtaca aggcttactt
    14401 attaaagtta agaccttgta ctttacataa aaaaaataac taatacatag ttattctaaa
    14461 tttactattc aactggtatt tctaattctt tacatctaat tctaacttgc tctctaatta
    14521 ctgctggtac ttcttctatt gtttttttgc ctcttaaaat taaagctaca tatacttcta
    14581 ccattccact ctcacctcca gattttctta cactaaaagt gcttatatta cttgcttcat
    14641 tgacagaact ttgacttaac aacatctcat atatttctgc atttgctata aaattatttg
    14701 ttatatcatc atcttgcttt acttgaccta tacttatgtc tgaaatgtct ttatactgca
    14761 tttctacttt tctaccttgt ttgatttgtt ctattttatt tttatcgaat ttaaacaatt
    14821 ttcacacctc cattttaagc ttgtggctcg ttgttttcag catcatatct aaaaatcata
    14881 gatggacgtt ttatagctgt ccatttatct cctttttcta ctatgatttt aggctgagca
    14941 ctttttatgt tggcttcttc taattcatag atttcttggt aacttgagtc tgattttttg
    15001 atttcaacta gtttcatgtc tttgaataag tcagatttat ttatttttac ttgaacttta
    15061 gttttattta ttaattgata tataggattg ttttccatca tatcatcctt taatccctca
    15121 tttaatgtta ctattaaatc agtgttattt tcagtaatgt ttatgattga atatacttta
    15181 ttgtttatca ttaaaataga atcctttttt actttaagcc ctgtttctga tttaaatata
    15241 aacatacttg aatataaatc gcctgtagga ctactggctt taatcagtat cttattttct
    15301 cctaatacta ttttattttg tggtatctca aatataatgc tatttatttc caaattagtc
    15361 ttagtagata ttaattcatc atttataaac atttcaagtt tttctataac attattaggg
    15421 tcttgtacta caactctcac ttgagatatt gatttttcaa ctatttctat agatgcttta
    15481 cttgtatcta cttttgttat catgtatatt gtatcatctg aaagctctat atctaaaata
    15541 tcatttagat gttcaggaac tggtatttta gtaaattcat ataaatcagt aattgatata
    15601 gatattttat acatagattt atttttacca gttatccata gactattatc atttttaatt
    15661 actagtatgt ggtcatcatt atgtgactta atttctttaa cattagttaa tactttagta
    15721 aaagttgtta ttattttatc tttttcactt tctcctagtc caaacaatcc atttgtatta
    15781 tacccttgag cgtacaactc tccctcatta gttaaaacaa gtgtcattga gttatatata
    15841 cttatatctt taacagaagg taatccagta accttttgta actctcttac atcattattg
    15901 tgaccaactc ccaattggta ctcagtattc tttccatatg accatagagt attatctgtc
    15961 ttaataatat aggagtagtt atctccacaa gcaacttttt tgacatctga tatatttact
    16021 tcagtaaaag ttaataattc tatatcttca agagtttgat taatttcttt atatgaatta
    16081 cttcctattc catataattt tccatcctta gcaacagcca atgaatgtgt ttttgatata
    16141 tcaatatcag atacctcaaa tggcaatatt attgaagtat acttaaaatt atctttaaat
    16201 atgtttttat aatttatata cttattattt gtttttatat acttatcatt aaacattact
    16261 agcttttcat ttttcttatt aaaaactatt ttatctgatg tgttatctgt tttaaaatat
    16321 gaatgtaggg aaccttcaag agcttcattt ttatcattat catatctatg gtgaatacct
    16381 aaatgattat atctgttatc tcctgttgaa gaataaaatt ttccatatat atctttaata
    16441 aatatagtat tgtaactaga aaatacagta tcaacatctt tctttataga tactttataa
    16501 tatggaactg gtatatccat ataatactca tcagatatgc cataagtatc tctacctgat
    16561 gatatcaaat tgttattatt agctatataa agtgtattat tagctgtcac aaaaacatct
    16621 ttaacatctt ccacactatt tgtattgtta ttatttgtta tacttccata tccactaaga
    16681 cctccaacaa atagtttatt atcatttgtt aaaatggctg aagtatatcc attaactact
    16741 attttttttg catttataat attattaatt actcgtatac cctttatatc attagtttct
    16801 gttgaaaaga ataccctctc ttttttccct gtagtataaa tcatattgtc atttgttata
    16861 aaaaatgtag cttcactgcc tgctgtcaca acagatttta cattctcaat atttattttt
    16921 gtaaatgtac ttatactact ttcagtgaat ccaaaatatc catttacaaa tccacataca
    16981 taacatgtat tatcattttt gacaatgatt gatacatcat tacatagata tacatctcta
    17041 acaccattta taggtatacg tgtaaatata ttcctacttg tagtatcacc caatcctaac
    17101 tgtccttttc cattattacc tgctccatat aaaagaccat cattagttaa taatagagat
    17161 gtgtttccat atacagttac atattttaca ttttcaacat ttattttagt aaatacattt
    17221 ctatttacat tatctcctat tcctaactga ccagaattat ttaaaccaac agcataacag
    17281 gtattatcat tttttatagc aaatgcatgt ttactgttgt cagtaaatct atttacactt
    17341 atagatttta tattatcaat atttatttta gcaaatacct ttctatttat ctcatctccc
    17401 actcctaatt gacctacatc atttaaacca acactgtaaa gcatattatt attagttaat
    17461 aaatatgtag actttgaatg gctatatatt tcttgtatat tagaagatat ttcactactg
    17521 ctattctttt tattgtaatt tatcttatta tttaaattta aactactatt aactgatata
    17581 ccatgcgtac ccttagagtt atatccacat ataaataagc tcttatcagt taatattgca
    17641 aatctagttt tatctctact taaatcataa ggaggtataa atatattttt tatatttttc
    17701 attacctttt tataattagt ggcattatat ccatcttgtg tatctgaaac atctagttct
    17761 tttgcacttt cagatatatt cccaaaacca tatagcgtgt tagaagataa atacaataga
    17821 gctttatata aaaatatggc tttatcagag gtaataggac ctgaatttat attagatgct
    17881 gcaatattat tagatgattg attgttaaga taattccttc catattgatg tatataagat
    17941 gaacctccag aaataagtaa attatctgga tttacatttt ttaaattact tgttgattct
    18001 ccataataca aataggcaaa atttgtagaa tatgatgatt gtgtacacct tgtgggataa
    18061 tagttatata tgtaattact aatatatcca gcatgtgtaa ttccttgttg ttgaactcta
    18121 taagtagaat cacttggctt tgtccataaa gtaccatggt atccagttaa aaaagaacca
    18181 taatttgtac ataatatagc catatattca tatataataa tattattaat tttagtaata
    18241 tcatttgtac aagaatttgt actataactt gctcctgaag gatttacata aaaccagtta
    18301 tttattaatg ttaaatttat attgactcca tcattaggat taacacccaa taaatagttt
    18361 gtatttatac cagttgcata tacctttttt tcgtcagcca caataaatgt tgcattatat
    18421 gaactatatg catctgttac attgctaaca ccaatctcag tatattgact tgctgattta
    18481 ttactaagtc cattaacata acctgaactc tttccagaag tgtatatttg tttattatta
    18541 gttacaatta atgaaaacca gtttgataaa tcagtattta taacaacttt ttttacatta
    18601 tctatagata ttttcttaaa tacatctgaa ttagaaggaa gaacacctgc tattgagtta
    18661 ctatttggtc cagaataata acaagtatca tcatttttaa tatagaatat tcctccaaaa
    18721 atattttttg atgctactat agactttaca ttagagatac ttaattttgt caattcattt
    18781 aattgacttg gtaagtcaga acccattcct ttgtatctat tatttcctag accatataca
    18841 tctccattcc ctaaaataac catagtacaa taaggactaa aaacgacatc tcttacagaa
    18901 tgtatatcta gtggaagttc aatatccttt atttccattt cttcttttaa taaagttaat
    18961 ttatcctcag atttaattaa atttatataa tcactataac tatatttttc actatccatt
    19021 ttcttaaatt ctacaatgtg catatcatca aaaaacgaac ctaattgtcc ataagtatta
    19081 tctcctgtac aatatgaatc attatttgaa tcaattagta tgctatgtga taaaccaatg
    19141 aaaatattct tagctacaat attttttaaa tctataaatg aactatctat attctcagag
    19201 taatttgcta tacccaactg gtatatatca ttatttccag caaccttcaa aataccttta
    19261 ttatctataa gcatagtatt accatgacca catgcaatag tttttatatt tacttcttct
    19321 atcttagtaa atagtgatga ttgaagtgca tttccaaaac atttttcctt tccacaacca
    19381 tatatatcat tattatacgt cattaacaca gtgtgttcag aaccacaagc tatagtctta
    19441 acatcattaa tagaaactaa actaaataaa tctctattat tcttatctcc taaacctagt
    19501 tgaccttgat tattaaaacc agtagaatag catttattgt cattctttat tataaaagct
    19561 gatttattat atgaaaatac agcctttaca ttgtcaacat ctactttcgt aaatgtattt
    19621 acatttgcag atgctccatt agttcctaga cctaattgac catacaaatt atttccagca
    19681 acatataaag ttccatcatt gtataaaaga tatgttgatt gaagtccaca tgcaatatcc
    19741 tttacgcctt ttatttctgg aaccttctca aatgtagaaa ctgttttccc tactttttct
    19801 aatcccaatc ccatttgacc atctgtattt atacctgcga caagtaattc tccatctgaa
    19861 gtaagtataa ctgcatgaga ttctccacat gcaacttttt taatattggt agatatattt
    19921 ggtattttcg taaataattt tcttgttgta ttgtcttctc ccaatccaag ttgaaagtta
    19981 ctattatctc cacatccata caagatatta ttatttttta gaccaaatgt atagttacaa
    20041 catccagtaa aaaaattaat aaagtcataa ttatcatacc cttcatcaaa ttcatcaact
    20101 accatatttt tatcgccaaa gaaattacct ctttgaagta gtttagttct tttcattata
    20161 aaaacctcct aattatctta tttcatacga atatttattt aaagttatat ctgtctgacc
    20221 aattgaatat gccctattag attttgtttc aacaaagtta ttatccattt catgtatata
    20281 tctaactcta tttgcattaa atggtagcac cacattctta aatgtaacac taccaagttc
    20341 tccagttata cactttctat ttaaatctaa cttaaggcct tctatagcta ttatgttttt
    20401 agcattagtt aaggtatcat accaatatcc agcttcatct acatctattt ctgactctaa
    20461 ttctaagtat attaagatat ctaatacttt ttcagataat ttattgtaac tattttttaa
    20521 tacagatagt tcagtttgta tttgacctcc tattacatca tcaatagata tatttttaat
    20581 tttttctact acctcattta tagctcctga taagttttta gcttctgtag ataagagttc
    20641 ttcgccaatt tttactttta tttcttttat atttttatca aacttatcat ctaaatcttg
    20701 aatatccttt tgagttacaa aaaccaacgt cgggtctact tctaatgtaa catttgaagt
    20761 attggacaat tgtaaaatca tttttacaac caaatcttta atactgccat cttcagcacg
    20821 tggcttatac gtctctggat acttacctat agctattata ttattctcat tatccagaac
    20881 agcagcttct cttatcataa atccaccaac tgttcctggt aaaatagtct ctataactat
    20941 ccaattagga tttttttcat caattcttag agaatttata tttccttccc aaactacatt
    21001 ctttaaagca gtttgttcct ctgttggctc ataatattct ccattactat ctccaacttg
    21061 aaacttggtt aatccaacaa gctctcctag tgcagttgca tttgctattt tttctctacc
    21121 aattttggtt aatattatat aaaatttttc ttccaattat accacctccg atttaggata
    21181 aactactaca ttttctacac ctgtatcatt tgcaatagct atattaatct tacaattatt
    21241 ttcaatgcat ttaatgctta aaggataaac ctttatttct tctctactta tcattccagc
    21301 tccacagttt acaacagcac tacattttat aattggttca aagtatggat gaacttttac
    21361 ttcttcacta cataccatac ctccaccaca atagaccata cttttatcta gtattattgg
    21421 ttctaaatag tgtaataagt gagcaggttt tactctttca atcatatcgc tacaatctaa
    21481 aagagtttta tagtcacagt tatttgctat aaaactcaat acgaatgtaa attcatcact
    21541 ataaactttt atatctgttt ctgattttgt atatgcctca catatacttt ttataacttc
    21601 aatagtacta gtacctctgc ttctcatttt agcttttata ttgctacgtc ttgtgtcaaa
    21661 atcaagttct tttttttcaa tgcagagtat gtcttcccac atatctaatc cccatgttgc
    21721 agtgtcaaca aataattggt taaaggtatc atcaatatct tctttaagaa tatttaattc
    21781 tgtatcgtat gcaccttgta tctcctctac aatgtaattt ctatcaaatg atggtagttt
    21841 atcaattagc ttcattgatt ttccacctca ctaaaattaa catttgttac actaggtatt
    21901 ttatcttcat taatagttat attatctgta cttccattta taagtaggtt acttaaatcg
    21961 tgtacaccag tagtatttat aagtattccc attacttttg tatagattat ctctcctcta
    22021 atatctctaa agtatgtatt tatactttct aggaaagatt cttttacatt atctaatgta
    22081 tatccatctt ctagtttcat tactgcactt atacttattt ctattggtaa tggtgtcaca
    22141 actgttatag ttggtccaat aggcttctct tcatctatat gttgctgaca tctttcaatc
    22201 gtttctgtat caacagcttg attatttttc ccaaatatca agaccttaac tgttcccgga
    22261 ccatcccatc ttggataaac cttaacatta tagactccat ctacttccaa agcccattct
    22321 tcatagtgag ctttatttcc acttgtagct tgatttcttt ggattttata aaatctttct
    22381 tttagttctt catctgtctc tatatctgta ccaccttcaa aacttcttgt gttagtaatc
    22441 tttgttactc cagatatatt attaactaat ttaaattcac aatttgtagg taaattatat
    22501 ttcttaccaa cttccagagc ttgaactggg ctattgtcac cttcttcact accaatagtt
    22561 acatctttta ttactacaaa tagtaaatct ctatatgata ttactgtgcc attatttatt
    22621 acagttcctt tctctccaat aaattcaact tctccatttg actctgtacc taactttcta
    22681 tatacaccaa attcattgac tcttttatca agaaactggt tatatgtgtc ttgtataaaa
    22741 gccattttat gtatctttga aagttctaga tatatcttcg aaagttctag attatttcca
    22801 gataccatgt tgtttagaaa agaaccttct cctttataaa tatcaagatt aatattttca
    22861 agagttctat tttttattac ttcatatgtc tggtcactat acatttattt ccacctcccc
    22921 atagatagtg gatactttta catttgcact caatatgtct ctgttaaagt tagcactttt
    22981 tacgtttact tctaatatat atggatttat tagaagggcc tcttttatga atctactagc
    23041 ttcactttct gtaagacctt ttgtatattt ttgtcctatt agttctgaaa gctctgtccc
    23101 atattctaaa gagtatatct catgttcata tctatttgtc tttatacact tgtatatcca
    23161 aacttttaag gcttcttttt tttcaattat tttaaagtca cctttttcta aaataggttc
    23221 atctttttca aaatcccatg ccacttcacg aaagattggc aattcttctg ttttaggtaa
    23281 gatataatcc tctgggacac ctataaaagg aaatattgta ctcattataa gctcaccaac
    23341 ttacttacaa cagcaaattt ttcgcctatt ctaaacatta ttaccatatc cccagattca
    23401 aagttatcta taaaaggatt ttttacttca tgctggtgtt cttgatttgt ttctgtgtca
    23461 aatgtttcta gctgtctatc aatcatccaa ctatctatca aaatatcttc tttttctaat
    23521 atgatgttat ttgtctctat ttttaaatct ggtaatttac ttttaatttt tccaataaaa
    23581 aaagaaggtt gattatgata ctttccttct tctcttatta ttcctataaa ttcatttatt
    23641 ggattagcca ttatatcacc atccttttat aatactctcc tagctgtatt aaaatctctt
    23701 ctactactta acttacttat tttaactaca tcaccttttt gtggagcatg taaaaattct
    23761 ccattaccta tatataatac aacatggctg actggattat gaaaaaatac taaatctcct
    23821 gcttgaagat ttttttgttc tactttcttg ccttttttag attgttgatt tgacgttctt
    23881 ggcaaactaa cattaacttt tttaaaacag tagaccatta aaccagaaca gtcaaaacta
    23941 cttggtccat ttccacccca tttataaggt ttacctagat gtttttttgc ttctgatatt
    24001 actattttag ctttattaga tgcattacca gaataaccac cgataataat ttttcctttt
    24061 ctttttccaa atcttttcat ttcttctgca ttttttacta aaacatcgat atgataagtt
    24121 ccatcttttt ctataattat agcagaacca ttatcattta ctttatacag tccatcacgt
    24181 ccagaaactc ctgttacaat acttacttca tcaccatatt taaataatgg gtgttttgat
    24241 aagaactctt ttgtataata aggtttttca taagctccaa ccataggtgc agcacatgtc
    24301 tttttagaag gattaagctt tttctctcta cagtctgtat ctccaccttc tataccaggt
    24361 ccaggataat atgctgtaaa tatagcagaa aattctgtcc ctcctgcata atcttctccc
    24421 cctaaattac tttcttcctt aggttcatcc tgtcctgctg acttttcatc cataagattt
    24481 tgaaaattaa gttcaagctc aatttgatac tctccatttt gccaagtatg tttgtctgta
    24541 tctatataaa atagtcctac aagctttgta taagaatctt taacttttac tcctctacca
    24601 gttatacaac ttacatctcc ataaccttta agagaacagc ttttttctat cccattaaac
    24661 tcgctatcaa tatctacatc ttgattttct tgttgctgaa ttactttttg cattattaca
    24721 tttacttcct taaaaatttc attatctatt tttttgctaa tcttgcttcc atactggtct
    24781 accactatta ctttattttt tacattttcc atactctccg aaaaggtggt atttataata
    24841 ttaaatccct cttcaaacat aacacttaat gtaacagttc ctttttcaat aacattaaac
    24901 ttatctaaat tggcctctat catatacttt ttctttgtct ttttacttgc ttctgtatat
    24961 gcactcatta ttgtgtcata accatttact cctataaaca tctttgtata cttgacattg
    25021 gtctttgcta ttgttccaac tgaaagccta ttttcagcaa atacttgctt tgctatgtct
    25081 tcaactaact tatctttaaa attgtatgac acttcacttt gtgtaagtaa aaatcccata
    25141 tctttagata caaaactaat ttcattactg cttgaatctt tagacctatt aattatcatt
    25201 cctctaaaga gttctttctc atctacatag aaacagactg tactagctat tggtatatcg
    25261 atttgttgga aatttacatc aaatgatgat tgtattattg aaaactctag tgtccttgat
    25321 ggagatttat aatcacctga ccatgatacc ttgtctacta tgtcagttat gtcatatata
    25381 cttccgtttt ttatgtgtac ccaaatctta ataataaaaa atcacctgcc ttttcattta
    25441 attctatcag cagttattta taacttatta tctataatag ttattgtttt ggagttactt
    25501 ttggtatctt tattctttta tactctttta aatctaatgt aaaatataca tctcgagtcc
    25561 catctctttc ttcataacta aacccttcaa ttatgacttc catatttata tttgtttccg
    25621 taattgtaaa tcttaatata aaaccttcct tcatccattt ttctatctta tttacacaat
    25681 catatggtga tggaaaacct gtatagtcac agaaagtata gtctttattt ggaaaaaaac
    25741 ttgatattga aatgctttta agagctacac caccaaaagt tgctatttca cctattttga
    25801 gtatactaga agagtttaca gcagcttttc cattaatact aaaggaagat ggaaatactg
    25861 gaaatctaaa tctatcttca gcttgtctaa gccacatttc cattataaga caccccctaa
    25921 ctgtgccaat tggatacttg caactaattt tgatgttatc ttttctatat cagcttcttc
    25981 tctaatcact attgtatcag ctaacttagc tatgtttata tttccaccat tttttctgct
    26041 tctatattga tttgcttctt gtttagttaa aaccatttca ccttcatgga gtctggtttg
    26101 atagtcatta taaggaacat agttcaatcc aaatgcatta ttacctttct tacttgaata
    26161 agctttacca gaatcaccag cctttgttcc agcatatgga ttatctttgt aatttttttt
    26221 actcttttcc actagattta aaactgttcc tgtgatgctt ccatttttta tcttattaat
    26281 catagtttgc caccatttgc atatatcttt aactatttta catactttat ctaatatgtt
    26341 aaaaaaagct cccaatattg gctctataat tttccatcca gtttccaaca gaggtcctaa
    26401 ggtctcccat acagttttcc aaataacacc cagtgtctct ataaaattgt ttatttgagg
    26461 tgcatattca cctaaaaaag taaaaattct tgtgataata ggactaacag cttcaactac
    26521 aggtccgatt agttggaatg ctgtagttac tattgtacca aatatactta ttaatccttg
    26581 tgcaattggt gatttattgt ttatactatc tagtaaatta tttactaagt ttactactgg
    26641 aagtaatgaa tctattaatg ctgtaaaatc gatattttgt aatagtgtac ttcctgcgtt
    26701 aacaattgct tttggtgcat tccctatcat tgttgctgca tgattttcaa aacgttcttg
    26761 cttattttca accatcattt tacgcttttc agcctcatta gaaattcgat attgagtaga
    26821 atcagaaaac tcttttccat tttttacact tctagattgc atctggtcat atttgttgcc
    26881 aataattatc ttaccattca ttacattgcc tattttacca gccatttttt cagataagcc
    26941 agctaaattg cctaatccac tttctataaa agtttttatt ttctttagtc cttgacctaa
    27001 aatatcatca aaaggtttaa caatactctt caagccattg ttaatgcttg tttgagctcc
    27061 tgatattata cctggtattg tattactcat ttctttagct aaccccatct gctttacact
    27121 tatgccttcc aatgcttcat caaaagtttt ataattttct cctaccagag tattaagaga
    27181 ttccatgttt ccttttgctg cacttaagaa agctgctgat acatcttgtt cactacttgt
    27241 attcatattt aaagctctaa catctaccat agcttgagtt atatcttttt cttttttgcc
    27301 accagtaata ttactaatca ttttagcttt agttcccatt gcaacaacat cttcagggtc
    27361 aaaaggcgta tcatttgcca ttcttactaa actgccataa taatcatcaa catctatttg
    27421 attaactttt ccagtatttc ttttcattac actttctaga acaactttct gattttgttc
    27481 ctgaccaagt aaatcaacac cctttttcac agcgacacct atagtagcac catttgtcaa
    27541 atcagatatc ttccctttta tcgaaccaag tattccactt gccatatcct ttacagctat
    27601 tgcaccactc cataccttac ctgcaaataa tctcaatttg ggaaatactt tacttataat
    27661 accacttgcc atatctttta tagctattac accactccaa aaagtatttt taaacaacat
    27721 taacttaggc aaaattctag ccatagcact acttaccata tctttaagat taatacttcc
    27781 ttgataagga cgtttctcca ctcgttttaa tttttccaag atactactta tcactctctt
    27841 agtcatatct tttgctttta ttatgatatt ccaaatcttg ccaccaatct tttttaaatt
    27901 tccaagtaat ttatttttag ttttttctac ttcatcactt ttagctttaa atattaatat
    27961 aatcataata tttgaattaa actcattttt cttttgaatc aacttattaa aataatcaat
    28021 aatcatagct atagttattg ctagtctcat aatacttcca tttaaacttc caataactcc
    28081 tgaaacagca tttatgctag aacttaattt tgtcatagtt gaacttacac tactagcagt
    28141 agtttgaact tttcttaacg aggaatttaa actgtttact ctactacttg cacttccaat
    28201 agaatttttt atgctactag ccattacatt agtggaaagt gaaacagcca cagttgctct
    28261 cctaaaatta tcagttactg atataatttg attcattttt ggtgtgtaat tatctgtaag
    28321 gtacatattt ataccttctt ctctcacatt tcccattaca taccacctcc tgtcaaagga
    28381 cacactccat atccatgttt cattttttca acttcttcct caatttctaa ggcgaaaaaa
    28441 gcttcaataa gctttaattc gcctttatcc attgcataaa aaagggacgg tcttatacct
    28501 tttttcttcc aataatagta cattattgta gtcctggtat ccgtccctat tagtttttta
    28561 cctcttctat cactgcattt ttaccataac ccataacatc ttgaagtatt ctatataagt
    28621 tggtaatttc acctggtaat aataattttc ttgctaattc ttttggtgtt ggagccttaa
    28681 atttatccat tagctcctta tttttaaata aaagctttcc atccaaatca caaacacctt
    28741 ctataattgt aagcatctgc atctccggta agtctatatc cgttttcata ttttcatcaa
    28801 ttttaagaca agtattttgt atctcttcat ctttacttat tgttaaagct ctacacatta
    28861 ctttaaattc ctgcccaaat atagttgata atctcttaag ttcaactata gtacttggtc
    28921 tttctaagtt ttctgtatct gcatttaaaa gtaaatctac tatgtttata ttttttgata
    28981 atccattttc actcattatt atttgctcct ttatcaatca aattcaatca actaactttt
    29041 ttacttatta actatctgaa tttattaagt atatatttac taaatatcag tataaaacca
    29101 aatataaaac taaatataaa actaaattat atcaagaaaa tcatactcag taaatgtgaa
    29161 aggtgcttct atctctcctt ttacgccaac ctcccaatca gctaaagtta aatcatcaaa
    29221 tgctatattc tttactgcta ttctttctgc tccataagaa tctgggtcat ttaatttaga
    29281 tatagcaaca aatctaggtt cagaacctct ctttatcttt tctccaatta acttgtgcat
    29341 tcttgaacta acatggtata gagttattga gccttttcct ttatatccca tatactttgt
    29401 atcagtaccc atttgacctg ctattataat atcttctttt gtaaattcca tctttgcttg
    29461 aaacttcttt acttctgcta ctttgtttcc atcaagccaa agttctcccc aagtaccact
    29521 cattacattt ctagcttcca tatttgccat attaataatc ctcctatatt tctattgata
    29581 aatctatatc ttccatagca tcaagtactt ttatttttgc ttttaaaaat actttagaac
    29641 ctgtattagc ttcttttatt tcttgtaatg tcatataaga taaatctact ccttttgatt
    29701 ttaaatacga tttttgtgct tcaaaatcta tttcaacagt agaatcagat tctataagtg
    29761 ctgatttttc taattcttct aaataacttt ttatagctac tatcaataaa catttgttgt
    29821 cataactgtt agtaaccttt cctatatagt catctattat cacctttctt atgtcactat
    29881 gtataatatc taaagtgtca actattttta ttttctggaa catttctcct ttttctgctg
    29941 ttaattcagt taaagaattt actcctctag caattcttat agctcctgct tctttaataa
    30001 gtataagctc tcctttatta acctttgatt ctgcatcaac tttcgtcatc ttaggtatat
    30061 cgactacatc actaagttta gtataagtta ctgattgact taaaggtgta cctgctataa
    30121 gtccggccac cctacttgta aactcatcaa cactgtattt cttttctcca actaacacat
    30181 cttctgtagt aaaattaatt atcccttcat gatttcctac aacttttcct aatacagctt
    30241 taaccttaac cttatctata tctctaagtt taattatcca attttttata gcagtcttat
    30301 cagcttctac tgcttttggc atacatagat aattaaattc cttagtctct aaaaaatcta
    30361 atgcagtttg aatatctgct tctccttcta ttacataaac taataattta tttggagtgt
    30421 taacatttcc catcaaagct aaatttatat attctttatt ttcagcagat aaatcaactg
    30481 gtatatcctc tttttcatgt atttcattaa gacctagtgc cttagcatct ttaagcacca
    30541 ttgcaattat tcctctagct gaacgttctt taacagttgt agctagctcc ttaaatgata
    30601 tgttgatact tggtaatcct atagccatac tatcactctc ctaaatttaa attgatttct
    30661 tccatatttt catatactac atcttcttca tatacactat ccagatagtt tagtgtaagt
    30721 gtaaagttca atacataaat tgactcttct tttttaatac tttgttctag ctttgttata
    30781 tttaattttc tgtctgttac ctttatattt ctgttgaata acttatttaa ttcatctgaa
    30841 atttcataca ttctaatact ctttgactgt ggttttggta agtattttat agagataaca
    30901 atatctttgt tattcgtatt taacattaca ggtgtacaaa taactgatag tatacttaca
    30961 aaaaaacaag acccttcaga gccttgcaca ctttcatcat caataaaaat actttcagta
    31021 aagttttttt tgagaatctc aataattgtt tctaatattt ccttgtattt aagcaaataa
    31081 tcaccacact atcactaaat attcttgctt aatctgataa tcttactata aatgatataa
    31141 gtcattttta aatatatatc tcatttattc ctgtcaagta tcttctatat ttttaggtaa
    31201 tattattctt ttcaatctat cacctccttc aatatggatt tctttcacaa taacattatc
    31261 tcacatattt tattgcctat tgttgcaatg gtgtatataa agtgttgtaa aattgtccta
    31321 ttttttataa tatctcactc aatttaaata tcatcttttt ctttaaattg tagcaagatg
    31381 ttctatctaa gtgtagtttc atagaaatat atgtcatatt gttttttgtc ttactattat
    31441 aaaaaagatt aaaaaattcc atttctatat catttagaca cgttagagca ttttctatct
    31501 tctctttttc tatttctata tcttcttttt ccatttttaa ttttcttaat ctttcctctc
    31561 tttttaacac ctcattttcc acagaagagc ttatattata tgtgttacta gtcttttctg
    31621 tatataccat tgctccacat ccagagtatt cattctcaca tctttttatg tccaagttaa
    31681 tattctttat ctttatatcc aaatacttat aattatgtaa tcttttatct gtttctttaa
    31741 ataatatatc ttttttgttt tccatagtac ttctcctttt ttatatactt attattagct
    31801 tcaaatttac aataatctct ttagtgcaca gttttttact aatctcatag ctttcaagaa
    31861 catattgttc tataactgta taaaaaaaac ttcttctata ttttgttcta aaatactaga
    31921 aatcttctta gccataagaa gtccaggatt ttttatgcct ctttctattt gagatatata
    31981 atcatttata attccagcct ttctggcaag ttgaatttgt gtcatacctt tgcattttct
    32041 ataatatttc aagttatttt taaacattgt tttatacctc cattactatt aagaatattt
    32101 tgttcttcct cacttagatt atatagaatt atttgttctt tatcaataga ttttagaaaa
    32161 aaatattctt ttatatttta ttagttttat tttttagaac aatttgttat aatctataca
    32221 taaggtggtg ttataatgtt tgccaaaaga ttaagagaac ttagaaagga atttggattg
    32281 actcaaagag aacttggaga aaaagtaggc gtttcccaaa gagtcttagg gtactatgaa
    32341 acagaaaaca gatttcctga tgaacacatt ttaaataaat tggctgatgt atttaatgta
    32401 tcagttgatt accttcttgg aagaacctta gttaaggaaa atattgatac agtagctgca
    32461 catagaaaaa atccacatga agagttacct gaagaagcac aagaacaact taatgattat
    32521 atagaatttt tactaaataa atataaaaaa aaataaacca tatgatgagc agtctatttt
    32581 tctgctcttt taatataatt caaaggcaaa catacattct attttagggg gatttttatg
    32641 aataatttgg ataagctttt tgaattagct tctcaagaag agataataat tcattatacc
    32701 acttatattg caggtgattt agaaggacta tatataaaca aacatggtat taaaattata
    32761 tcattactca gtaatttaaa acaaaactca aaaaaattga catccatctt agctgaagaa
    32821 cttggacatc atttcactag ccttggatac tatgtatcct cttataacga ttattataca
    32881 aaaattatta tagataagtg cgaaaataaa gccttaaaat gggcttgtga gttcttaatt
    32941 actgaagaag atataataaa cataattaac tctggcatca cttgcgttta tgaaatggca
    33001 gacatactta atgtggatat tactttcttt caaaaaagat tagaattttt atcattaaaa
    33061 aaacagtctt tacaacttgg aaataataaa tatttaatat taactaattt gccgtatttc
    33121 tacatatttg atccaatttc ataataaaaa tagtagtaat tttaactggc ttaacagtaa
    33181 aatctatata tttattttaa caaattttgg tttct
//
LOCUS       DAEMHW010000023        33059 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEMHW010000023 DAEMHW010000000
VERSION     DAEMHW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33059
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.023"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            87..1256
                     /locus_tag="KPK67_000911"
     CDS             87..1256
                     /locus_tag="KPK67_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2457912.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            1305..2156
                     /gene="gatY"
                     /locus_tag="KPK67_000912"
     CDS             1305..2156
                     /gene="gatY"
                     /locus_tag="KPK67_000912"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG2457913.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            2482..4383
                     /locus_tag="KPK67_000913"
     CDS             2482..4383
                     /locus_tag="KPK67_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2457914.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVAGNVSDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            4386..5429
                     /gene="ypdE"
                     /locus_tag="KPK67_000914"
     CDS             4386..5429
                     /gene="ypdE"
                     /locus_tag="KPK67_000914"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG2457915.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRYVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            5444..5749
                     /locus_tag="KPK67_000915"
     CDS             5444..5749
                     /locus_tag="KPK67_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2457916.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            5846..6181
                     /locus_tag="KPK67_000916"
     CDS             5846..6181
                     /locus_tag="KPK67_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2457917.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            6213..7535
                     /locus_tag="KPK67_000917"
     CDS             6213..7535
                     /locus_tag="KPK67_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2457918.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFRPITSPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            7545..8615
                     /locus_tag="KPK67_000918"
     CDS             7545..8615
                     /locus_tag="KPK67_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2457919.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            8620..9807
                     /locus_tag="KPK67_000919"
     CDS             8620..9807
                     /locus_tag="KPK67_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2457920.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(10004..10876)
                     /locus_tag="KPK67_000920"
     CDS             complement(10004..10876)
                     /locus_tag="KPK67_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBG2457921.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYKQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(11270..11461)
                     /locus_tag="KPK67_000921"
     CDS             complement(11270..11461)
                     /locus_tag="KPK67_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457922.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            11681..12727
                     /gene="cobT"
                     /locus_tag="KPK67_000922"
     CDS             11681..12727
                     /gene="cobT"
                     /locus_tag="KPK67_000922"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBG2457923.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            12759..13319
                     /gene="cobU"
                     /locus_tag="KPK67_000923"
     CDS             12759..13319
                     /gene="cobU"
                     /locus_tag="KPK67_000923"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBG2457924.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            13329..14102
                     /gene="cobS"
                     /locus_tag="KPK67_000924"
     CDS             13329..14102
                     /gene="cobS"
                     /locus_tag="KPK67_000924"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBG2457925.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAVYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            14122..14736
                     /locus_tag="KPK67_000925"
     CDS             14122..14736
                     /locus_tag="KPK67_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG2457926.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENNSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      14803..14982
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            15097..16611
                     /locus_tag="KPK67_000926"
     CDS             15097..16611
                     /locus_tag="KPK67_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBG2457927.1"
                     /translation="MGKKIMLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMAL
                     NSFITKEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSS
                     KYHDYKLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPV
                     IIVGDIDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKV
                     PVIGVVPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVS
                     IRYVDYGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQ
                     MLGKKLKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGK
                     KVKGYEIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEIS
                     FTRAILNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            16650..18026
                     /locus_tag="KPK67_000927"
     CDS             16650..18026
                     /locus_tag="KPK67_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBG2457928.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            18027..18998
                     /locus_tag="KPK67_000928"
     CDS             18027..18998
                     /locus_tag="KPK67_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG2457929.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFIIVGITYLTTYVIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            19030..20100
                     /locus_tag="KPK67_000929"
     CDS             19030..20100
                     /locus_tag="KPK67_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2457930.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTSLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIKIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            20102..20989
                     /locus_tag="KPK67_000930"
     CDS             20102..20989
                     /locus_tag="KPK67_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG2457931.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPIGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            20977..21609
                     /locus_tag="KPK67_000931"
     CDS             20977..21609
                     /locus_tag="KPK67_000931"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBG2457932.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            21618..22844
                     /locus_tag="KPK67_000932"
     CDS             21618..22844
                     /locus_tag="KPK67_000932"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBG2457933.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            22841..23449
                     /locus_tag="KPK67_000933"
     CDS             22841..23449
                     /locus_tag="KPK67_000933"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBG2457934.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRIDNFDMNVVVILDEE"
     gene            23439..24014
                     /locus_tag="KPK67_000934"
     CDS             23439..24014
                     /locus_tag="KPK67_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBG2457935.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGCAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            24007..24759
                     /locus_tag="KPK67_000935"
     CDS             24007..24759
                     /locus_tag="KPK67_000935"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBG2457936.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAYMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            24743..25864
                     /gene="cbiG"
                     /locus_tag="KPK67_000936"
     CDS             24743..25864
                     /gene="cbiG"
                     /locus_tag="KPK67_000936"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBG2457937.1"
                     /translation="MNIEGNIAIICITENGKKLAVKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDKLRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            25861..26586
                     /gene="cobJ"
                     /locus_tag="KPK67_000937"
     CDS             25861..26586
                     /gene="cobJ"
                     /locus_tag="KPK67_000937"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBG2457938.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            26583..27335
                     /gene="cobK"
                     /locus_tag="KPK67_000938"
     CDS             26583..27335
                     /gene="cobK"
                     /locus_tag="KPK67_000938"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBG2457939.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            27354..28832
                     /locus_tag="KPK67_000939"
     CDS             27354..28832
                     /locus_tag="KPK67_000939"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBG2457940.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKYQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFDVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            28862..29767
                     /gene="hemC"
                     /locus_tag="KPK67_000940"
     CDS             28862..29767
                     /gene="hemC"
                     /locus_tag="KPK67_000940"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBG2457941.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            29757..31256
                     /gene="cobA"
                     /locus_tag="KPK67_000941"
     CDS             29757..31256
                     /gene="cobA"
                     /locus_tag="KPK67_000941"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBG2457942.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNIKLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            31273..32238
                     /gene="hemB"
                     /locus_tag="KPK67_000942"
     CDS             31273..32238
                     /gene="hemB"
                     /locus_tag="KPK67_000942"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBG2457943.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            32391..32969
                     /locus_tag="KPK67_000943"
     CDS             32391..32969
                     /locus_tag="KPK67_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBG2457944.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKIYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        13378 a         3298 c         6112 g        10271 t
ORIGIN      
        1 aaaattataa aagagtatat ttttttatag tttaaagtgg ttataacctc attaccaaat
       61 gtaaatgtta caagtatgaa aggagaatga aaatgtttaa attagaagaa caaaaactaa
      121 aagatttagg tgcaataata acaactaatg aaataaaaca acagcctgaa ttatggttag
      181 agacttatga aatatataaa tctaacaaag aaaaattaag tagattcata gatacaataa
      241 gtaataatca tgggcagttt agagttatat ttacaggtgc tggaacttca gcttacatag
      301 gaaattcaat acttccttac cttaaaaata aaaatgacat aagaaaatac atatttgaag
      361 caatacctac tacggatata gtttcaaacc catatgatta cttaaaaaaa gatataccaa
      421 cattattaat ttcatttgca agaagtggaa atagtcctga aagtttggct gctttaaatt
      481 tagggaataa gattgtagat aatttttatc atttagctat aacttgtaat ccagaaggag
      541 aactagctaa gatgactaaa aacgacgaaa ataattactt gttattaatg ccatcaaaat
      601 caaatgatga aggctttgct atgacaggaa gtttttcgtg tatgatgtta tctgctatgt
      661 taatattcga tagtttagaa gatgatgtag aaaagtcata tataaatgca ataattgaaa
      721 tgggaagaaa tgttatagat agaaaagatg aaatacatga gttaataaat aaagattttg
      781 atagagttgt atatttaggt tcaggaggat taggtggttt gacacaagaa gcacaactaa
      841 aacttttaga acttacagca ggaaaaatat ctacagtgta tgattctcct atggggttta
      901 gacacggacc aaaatcattt attgatgaaa atacacttgt atttgaattt gtatctaact
      961 gtttatatac aagaaaatat gatttagatg ttttagagga gataaaaagg gataaaattg
     1021 ctaaattcac ttgtgcagtg tcagtagaaa atgaaaataa cttctcagga acaaagtttg
     1081 agtttaaaga aaagtataat aaattaccag atgtgtatct tgcaatgcca tacatattgt
     1141 ttgcacaaac tatagcattg tttgtatcgg taaaggttgg aaataagcca gatactccat
     1201 cagctacagg aacagtaaat cgagtagtaa aaggtgtaac aatatatgaa tattaaaaga
     1261 taagattaaa atattataac taaataattt gggagggtat caatgtggca ttagtaacaa
     1321 caacatcaat gcttaaaaag gcacaagatg aagggtatgc agtaggagca tttaatgtag
     1381 aaaatatgga aatggtaatt gctgttatag aagctgctga ggagttaaat tctccagtaa
     1441 tattacaaac tactccatct actgtaaaat atgcaggtct tgattattat ctagcaaatg
     1501 caaaggttgc tgcagaaaga gtaaagatac ctgtagcaat gcatctggac catggttcaa
     1561 gttttgacct tgcaatgaaa gctttaagaa gtggatatac atcaataatg atagatggtt
     1621 ctcatgaaag ctttgaaaat aatatatcta tatctaaatc agtagtaaaa gcatgtagtc
     1681 cttctcttat accagtggaa gcagaacttg ggaaagttgg tggaaaagaa gatgatttag
     1741 atggcgggaa tagtaatgat tatacaaatc caaaagaggc aaaagagttt gtcgatagaa
     1801 caggaatatc ttcattggct attgctatag gtactgcaca tggactgtat aagggtgaac
     1861 ctaaaataga tttagatagg ttatcagaaa ttaaaaatgt agtttcaata cctttagtat
     1921 tgcatggagg ttcaggaata catgatgata ttataaaaga agctataaaa agaggtattg
     1981 caaaagttaa ctatgcaact gaattaagaa ttgcatatag taaaggggtt aaaaaagttt
     2041 tagatgaaga ttctgaggtt atagacccta aaaagtatgg attagcaggg cttcaatcag
     2101 taaaggattt tgtaaaagaa aagattaaag tgtgtgggtc tgtaaataga gtataaaaat
     2161 aaaatatcct aaatttattt tagataagtt taggatattt gttatatgaa aaatttatat
     2221 taattatatg taaatggtgt atttttttca tttaattgat gaaaaatatc agcttttatt
     2281 ggtaaagtat tacatatata attaaaatat aataaaaatt gaactatata ttttattatt
     2341 ataaaatatc tatattaaat tttggaatta aaatatacta ggatataaag ttcttaaata
     2401 aaaagaaaag gttgttttta taaaaaatat tttttatacc actttaaaaa acacaataat
     2461 aacactgagg gaggtgggga tatgagagaa cgcattattc ttgaaacatt aaaaaatagt
     2521 agtggttatc taaacataga ttattttgca gataagttgg gggtatctac tcgaactatc
     2581 agaaatgaaa taaaaaagat agaaactata aaagaatgta atggttttaa attagaatat
     2641 aagtctaaat taggatatat tctaaatatt aaagaccaag ataaattcga gcattatcta
     2701 agaaacttac cctcagattt agttgaaaat ccagaacaac ggttggagtc tataatagta
     2761 gagcttttag taaatgaagg ttataaaact atagaacagt taagtaaaaa atttctagta
     2821 agttcatccc aaataaaaaa tgatttaaaa aagatagatg aaaagataaa ggatacagaa
     2881 ttaaaactag agcgaaaggc tcattatgga attaaaatag aaggttctat aaaagaaatt
     2941 caaagtatat tggtggatag ttattttaga gggaatagaa atataacaga gtacaggaat
     3001 aagtttatag ataatttaaa attagctaat attagaagta caataaaaaa tgtacttaat
     3061 gaacatgatt tagaagcaaa tttaacagaa cttgaggaaa tattagcaca aattattata
     3121 ttgtatataa gagttagtat gagagtagcg ggaaatgtta gcgatttaaa attatataca
     3181 gaagatttaa ttatagataa attattagat aaaattttta gagataaaaa atataacctt
     3241 aattatgatg aaaagtatta tttaaaggag cttataaaac taaagactaa agataagaaa
     3301 gccactataa agaatattga taagaataaa cttcaagata ttatgttcga attttttaga
     3361 gagatagata aaaaatataa ctctaatttt ttagaagata aagagttttt taacttattt
     3421 tacttacata ttgcatgttt aatagaacgt ataaagaaga accacaagat aataaatcct
     3481 ttttcagtta aaataagtca acagtatcca actgttttta atttatctat tcaattttca
     3541 aaaatcatag aaaatgagta tcatataaaa ataagtcaag atgaaatagg gtttatagct
     3601 actcacattg ctgttccttt tgaaaagaga gaagaagcta attttaataa aaaatataaa
     3661 atagcaataa tatgctcttc tggtggaggt agtgcatttt tgataaattt aagacttaaa
     3721 gaaatttttc caaatgctga gataagaaat ttttcattac tagaagaaaa agctgtaata
     3781 gaatttgcac cagatttgat tttttcaata acaaatcttt tatttgaaat aaatgctcca
     3841 gttatactta taaatgaaat tttagacgaa ttggattatt tagatattaa agaaagtgtt
     3901 agatttgcag accatatagg aaatatatca agcccaaaac aatatatatt aggtctattt
     3961 gataagaatc actttaggtg tataaaagat aaagctaagt ataaggatat ccttgatagt
     4021 atgtcaaaat gtatagttga cgagggagct tgttctccaa cctatcccaa agatgtatgg
     4081 gaaagagaaa gctatttgag tactatttat acaaatgggg tggctatacc tcatccaatc
     4141 gagatgacag gaaacaaaaa tataatatca gtagctttga tacaaactga tattatttat
     4201 gaaaataggg tgcccaaaat tattttcatg atttcattaa taaagggaaa tttagaactt
     4261 cataagcaga tatctaaata tttaagtaag attatggcaa ataaggatat ggtagacatg
     4321 ctaaataagt cacaatctta tgaagagttt atgtataagt taaaaatcta tctgggaggt
     4381 tagacatgaa tatagagttt tttaagaggt tgtcagaagc agatggtata gcatctaatg
     4441 aagaagaggt tagatatgtt ttattggaag aactaaaagc ttatagtgat aaaattatct
     4501 gtgatgggct agggagtatc atcttttcta aaataaaaga tgagagtgca ccaaacgtaa
     4561 tgatttgtgc tcatatggat gaagttggat ttatggtgag aagtatagac aaacttggta
     4621 tgattcactt aataacaata ggaggagtaa aacctcttgc acagtttgta caaaaagtta
     4681 gaattacaac aaaagaaggg aagaaaatcc ccgctgtcat aaatgctaca tataataatg
     4741 gtaaggctga aaatatatat gcagatattg gagcatatac agaagaagat gtatataacc
     4801 tagggattaa tgttggggat atggtaacat atacaacaag ctttgaggaa tttactttac
     4861 cagaccgatt agtgggaaag gcttttgatg ataggatagg atgttttgtg atgggagaag
     4921 ttttaaaaga acttaggaaa gaaaatctaa attgtaatat tcactttgcg gctacaagta
     4981 gtgaagaggt tggaataagg ggagcaaaga catcaacaca gctaataaat ccagatatag
     5041 tatttgtaat agatgtagca tgtgctaaaa atgagtttgt gagagactat acaaatcaga
     5101 aacaaattga taaaggtgta atgctaatgc atagagatag gactctagtc cctagtagaa
     5161 aaatgataga ttatgtaatg gaaattgctg ataaaaataa tataccactt caacatgata
     5221 tgtttgaatc aggtgggact gatggaggag aagcacatct cgtaaatgaa gggaaacctt
     5281 gtgtagtaac ttgtgtacca gttagatatg gtcattgtgc attttcaata gtaagtaata
     5341 aagatttgga gaacattata agattgtata cacagttaat tttaaatttt gatgaagaac
     5401 aatataaaca tataagaaat tttatctaaa ggagaataag aaaatgtcaa cagaagtaca
     5461 aataatggaa cttatatcaa ctgcaggaga gagtaaatcg aaagcttttg aggctttaaa
     5521 aaaagtgaag tcaaaagatt ttaaaggagc aaaactgtta atagaggaag ctcgtaaaat
     5581 agatattgaa gctcacagga tacaaacaga attaataact caagatttaa atgaagatgt
     5641 tgaaagtcca gcaatgagtc ttttaatggt acatgctcaa gaccactata tgacttctca
     5701 gctagcaaaa gacttgatag aggctttgat atattgtctc gataagtaat tgtaatgtag
     5761 aaatagatta tttatgtaaa aattctttta aaagtttaga tgcttttaag agataataaa
     5821 aatacaaact ttaggaggaa ttattatgaa aatattatta tgttgtgcag gtggattttc
     5881 tactaatatg ttaatgcaaa atatgaaaaa ggtggtaaaa gagagtgcta aactcaatga
     5941 aaaagatttt gagtttacag ctataccagc agattcaata gaaagtgaag ttgatgattg
     6001 ggatatagta cttataggtc ctcaaatagc tcacaaaata gacttcttaa aaggaatatt
     6061 agagccaaaa aataagcctt ttgcagtaat agataaagat gtatatggaa aaatggatgg
     6121 agctacagtt atgaagttgg cattaataac ttataagaaa catcaattag ctcaaggata
     6181 ataaaaataa tgagggaaaa gggagattag atatgttttc aaaatttgaa gcatttatga
     6241 atagagtatt tatgccttta gctcataagg ttgacaatca acgtcacctt ggttctataa
     6301 aggctggtat ggttgctatg acaccattca caatattagg aagtatattt gcaatacttc
     6361 cagcacttcc aaatatgctt ggagaaaata atccaataag caaattcata cttaacaatt
     6421 cagcaatatt ggatttacca gtaagtcttt ccataggttt aataggatta tatgcttgta
     6481 tgtgtattgc ttttaatctg gggaactact ataaattata tataccaggt tgtgttgcac
     6541 tatcaacttt ttctttttta ttcctagtag cctcatttac accagatgga gctttagaaa
     6601 taggaaattt aggagctaaa gggctattta caggtatgat agcagggctt ggttctgtgg
     6661 agttatataa tttttgtaat aagaagaata tcacaataaa aatgccagaa aatgtacctg
     6721 attttgtatc taggtctttt gaattaatac ctactactat aatagtttgt ggaactttta
     6781 tagttgctcg ttttatttca ttaaaagtat ttggagaatt acctccacag atacttactc
     6841 aatttttatc tccattagta ggaagtatgg ataacccatg gtcagttctt gcacttaact
     6901 ttgctatatg tactatattc ttttttggaa tacattcatc agtatttaga cctataacta
     6961 gccctataat gattacattt atagctgaaa acatagcagc tatgcaggct ggagaaacaa
     7021 tacctcattt ctttacagca ggagcttcaa gtgcattctt tggatttaca ggatgtggaa
     7081 ttagtatagg ttgtgtaatt gcatgtatgt tatcaaaaaa tgcttcctat aagaagattg
     7141 gacgtgtatc attattccct gcattatttg ggataaatga accaatatta tttggagcac
     7201 cgataatatt aaatccaata atgtttatac cacatgtatt tggtggagct ataataggta
     7261 cactaccaat gttttttatg cactggggat ggttagcaaa gccagtattt gaccctccat
     7321 atgtaggggt attcttagaa ggatttttaa ctaatggaga ttggcattct atattagcaa
     7381 atgcattgca attagtatta gcagttttat tatattggcc attctttaaa ataatggaac
     7441 gtcaacaaaa taataaaaaa caaaaagaag aaactaagaa tatattctct aaagaagaag
     7501 aagacttatt ggatgattta ggattggatt tctaatattt tgttatgaat aatacaatgg
     7561 agagattagt atccttaatc tataaaagag gtgttgacgc tcttttgatt aaagcaaaaa
     7621 atactaaaag atatatagga gctttatcag gtagtggtgt atatgtgctt gttactaagg
     7681 ataaaaatta tcaaattcta gatggcagat atattgatga agcagataaa aaaacgactg
     7741 gtttcataaa aaggattgta tctcaaggtt cttatatacc aactataatt gagttattaa
     7801 aagaattaaa tataaaaaag ttagcaatag aaaaccaagc tatgagtata caagagtatc
     7861 tttcactaaa aaatgaaggt tttgaaatca atttaataac aaatgaatta tggaagctaa
     7921 gggctataaa atctaaagaa gaaattgaac ttattaaaaa ggcttgtgag attacagatg
     7981 aagtatttga tgaggttatt tctgaaatta aaaaagatat gactgagcta gaagtatcag
     8041 ctctaattca gtatcatgca ttaaaaaaag gtgcaagtgg aatgtctttt gaaacaatag
     8101 tggtatcagg agaaagaggt gcaatgccac atggaagacc tacaaacaaa aaattgaaag
     8161 tgaatgaagc tataacaata gattttggtg ttgtttatca agggtatcaa tcagacatga
     8221 ctcgtacaat atctataggt aaacctccta aaattattaa agagatatat gatgtagtat
     8281 tggaagcaca attatctgca atagagtcaa taaaggaagg aactagggca agtgatgttg
     8341 acaaagtggc tagaaaaatt atagataaac atggatttgg cgaatatttt aatcatggac
     8401 tgggtcatgg tataggtctt ggagatggtg aagttcccac tttaaatcca aatagtgaag
     8461 atatattagt ggaaggaatg gtaatgtctt gtgagccagg tatttatatt ccaaatgtag
     8521 ggggagttag aatagaagat gacattgtta taatagatgg aaaaggtgtt ccactcaata
     8581 agacatccaa agagtttata atattaggag agtaaaatta tgaaatataa ttttaataaa
     8641 gtattaaatc gtaaagaagg aaattgtaga aaatggagca accatgtgat taaagaaaaa
     8701 tttggcttaa atgaagatgc aattcctatg gatttagctg atatcgattt tgagtgtgct
     8761 cctgctataa aagaatctat cttaaaaaga gcatcagtag gagattatag ttatactttt
     8821 ataggagatg acttctatga tgcgattata aattggaata aaagaagatt tgatgtatat
     8881 atagaaaagg attggataaa attaactttt ggtactgttt caacccttca ttatatagtc
     8941 caagcatata ctaaagaagg agagggtgta ttgataaata cacctgctta tgaccctttt
     9001 gcagaggctg tagtaaataa taatagaaag ttgtgttgta gctcattaaa attagataac
     9061 aatagatatt atttagattt agaggatatg gaaaatcaaa tgaaatacga gaatataaga
     9121 ttgcttatat tctgtagtcc acaaaatcca tcaggtcgta tatggacaaa agaagaacta
     9181 tatcaagtgt cagagttatg tttaaagtat aatgtaattt tggtaagtga tgaaattcat
     9241 agagatataa tatttaaaga ttatagattt gtatctttat ggaatgctca tccagaaatt
     9301 tataaaaata gtataatctg tgtatctcct aataaaggct tcaacttagg tggattaaaa
     9361 acatcatatg tattaataag agatgtaaaa attagggaga ctttattaga aaggctaaaa
     9421 agtaattcaa taacttctcc aaatgtattt gcaataccag caatagttgc agcatataat
     9481 gaaagcgaag aatggctaga tgctatgact agttatgtag aggagaattt tgaaatagtt
     9541 tatgatttct ttgaaactaa tataccaaaa gctaaagtga tgaagtcaga ctcttctttt
     9601 ttagcctgga tagatgtaag agaagtattt agaaatgaag aagaatctaa agaatttttt
     9661 agacatgcaa acttaactat ggtagttgga agttattttg tgaaagatgg agatggattt
     9721 attcgtatta atgtggggtg tcctagagaa acattaaatg aggctctaaa tagaatcaaa
     9781 aaaacgtata taagtatgta ttgttaaatc ataaaaaaag tatctatggt ttatataaaa
     9841 acacattatt tagttatcaa atattactgg aattttgtat tccagtaata ttttttgatt
     9901 aaggaatatt attgaggaat attaaataat attatttcat acccttatat taacttatat
     9961 aaaaatattt tattattttg ggaacgggtt agctaacctt tatctagtgt atatttatat
    10021 tgtaagaagc agtaaaaact tttcctttac taagtttatt gataaacttc ttatctttat
    10081 aaattttgtt agaatttata ctatcagcaa gaccatacca aggttctata cataaaaaag
    10141 gagcagtaga attagtttca ctataataag gagtccaaat accaactaga gggaaatctt
    10201 ccatagatat agttacatat ttactaccat cattattaca tatagatact tcatctatgt
    10261 tggtgtatat caaagcatca ttcttgaata cttctggatt taactgtaag ttttttaatt
    10321 ttccaatagg tattatatca tctgtaaaag aaccatttaa attgattttt tctacatcat
    10381 ttttactttt aaactcaagg tagtgtttag aaaaatcatt ttgtttgtaa aaaggaaggt
    10441 tgaatgcagg atgtccacct atagaaaaga atatatcttt agaatcagta ttttttacaa
    10501 tccattctac ctcaatacta ttatcattta atgtatagtt tatgattagc tcaaaagaat
    10561 atggatactt ctttaaagta gattcattat ctgttaattt ataagttaca aatgtatctc
    10621 ctttatctac aatctcaaag tccatatctc tagcaaaccc atgttgagtc atgctatata
    10681 aattttcttc tattattgtt tcattatcaa aaagtttacc tactataggg aacaatatag
    10741 gtgattgtct accccaatat tttttattac catcccaaag aatgtttttg tcgtattttt
    10801 tagaaaatat tttagttagt tcagcaccaa aactgcttga ttcgataatt aaattttcat
    10861 tttgtaatat attcatttaa aatctccttt attatatttt atgagtatat tatactataa
    10921 agtataaaga ttattaaatt ttatttgata ttatatttga aaggtagatt ttagtatatt
    10981 ttgaaaattt tgttttgaaa atttgggatt atttaagaaa ttttgtatta taaatagaag
    11041 gaaatagata aattgtatag aataaaatat attaagataa gttaataatg ttaaatggga
    11101 agtgagttta tggaaaaacc aaatacaaaa gttgttaact ttcaagagta taaagacaga
    11161 aaaaaccaaa gagaagtaaa tagagacgaa cttttaaagc ttataaaaaa aattgtgact
    11221 tcaaaatagg gagttgttaa actccctatt ttaatgggtt tcataataat taagacattc
    11281 tgagtccatc tatataatct ttgaattgtg agaaatctga ttcactactt aattctatgg
    11341 tttctgaacc ttccgtataa aaattcttaa gatagatagt ataattatta tcaacacaat
    11401 catgataaat acatctatat ccatcttcat acaaactttt taacttagaa aaatcatcca
    11461 caatatcatc tccttaaagt tcttttaaat atatttttac aaaaagcatc taattgtatg
    11521 caaattgaca atgtaaaata aaggaaatga acataaaatg tagaataatt tttatatgta
    11581 tgtaaaaata aaaagaaaaa gttgaaattt ttagaaaata agagtatttt ttatctaaaa
    11641 agagagttta aaaaataaaa aaatgttggg aggataaaaa atgagtttat tagaaagtat
    11701 atcaaaaaac atatattccc tagataactc ttctattgaa aaaacaaagc agagattgga
    11761 taggcttata catccaactg gaagcttagg aaagatagaa gatatttgca tgcaattagc
    11821 aggaatattt ggcaatgaaa attttgacac aagtaaaaag gttataattg cttttgcggg
    11881 agaccatggt gtatatgaag aaggcgtagc acctgaccct caaaatataa caaaattaca
    11941 gtttccaaat ttttcgaaag gtctatgtgg agttggagta ataagtaaat ttgtaggggc
    12001 agatgttgta gctgtagatg taggaataaa ctgtgatgaa aaactggatg gggtattaga
    12061 ctacaagata agaaaaggga catcaaatat ggcaaaaggt ccagctatga gtaaacaaga
    12121 agcaattaga tgtttagaga taggtattga aatagcagag cagtgtatag aaaaagatta
    12181 taaggttatt ggtattgggg agatgggtat agcaaatact actccaagta cagcaataat
    12241 atctgtaatt tcagggtgtg acccgttaga agttactggt ataggtgcag gtcttaagaa
    12301 agaaagacta aagcataagg ctgaagtaat tagaaaggct attgaaatta ataatccaaa
    12361 tccaactgat ggagtagata ttttatctaa agtaggaggg tttgaaatag gctcaatggc
    12421 aggtgttata ttaggttgta gtgctaatag gattccagta gtaattgatg ggtttatttc
    12481 ttatgcagca gcattaattg catataagat aaatccaaaa actagagaat atatgatagc
    12541 atctcattta tcagcagaat caggaactaa aagagcatta gacatattaa aattagaccc
    12601 tctattaaat atggatatga gattaggtga gggaagtgga gctgctttag catttgatat
    12661 aatagaagct tcaaactata cttacaaaaa tatggcaact tttgatgaaa ttgacatggg
    12721 aagataaata taaaaattta atatgtagga gtgttttgat gagtaaaatt attctagtaa
    12781 caggaggagc taggtcaggg aagagtaact ttgcagaaaa actatgtttt gacagaaata
    12841 ataatacagc ttatatagca acctctattc catttgatga tgaaatgaaa gatagagtaa
    12901 aaaaacatca agagaataga cctagaaact ggaatactta tgaaatatat gaagatatat
    12961 attccataat taaggagata tataaagaac atgaaactgt tatactagat tgtgtaacat
    13021 tattagtaaa taatctaatg tttgaacaca atctaaatat agaagaatct agtcaagaag
    13081 aaataaacaa attagaacaa tacataaaag aacaagttgt caaacttatt gaagaaatag
    13141 aaaaaacaaa tctatacttt gtatttgtaa cgaatgagtt aggtatggga attgttcctg
    13201 ggaataaact tagtagaatt tatgctgata ttgtaggtcg tataaatcaa tatatagctt
    13261 cgaaaagtaa tgaagtttat tttgtagtta gtggcatagc tatgaagata aaggagtagt
    13321 atgcaattat gaaaagattt attttaatac tacagttttt gacaaggata cctataaaat
    13381 taaatgtagg atttgatgat gaattttata agtcaatagt atattttcca cttgtaggtt
    13441 ttgtcatagg tatattatcc tatttaatag gatggatttc tatgctttta tttgaaccat
    13501 ttatagcttc tataataata actctagctg gtgtcttaat tactggagga cttcatatag
    13561 atggattagg agatacattt gatgcagtat atagttatag ggataaagaa aagatgcttg
    13621 aaataatgaa agattcaaga ttaggtacaa attcacttct agcaattatg tttgtacttt
    13681 tattaaaggt tggattcgtt tacgatataa taagtaataa ttcattgtgg gttataattt
    13741 ttatgccaat gattgcaagg ttaggagtaa tgttattgac atataaaact gtgacaccaa
    13801 gagaaaaagg aatgggaaat ttatttatag gaaaattaac tacaagtatg ttaataactg
    13861 ctataatata cacattattg atagttgctc ttattactaa atttatattt ttattaccta
    13921 atatagtgtt gattaaagta ttaggttcga ttatagtggt ttttgtattt ataatattat
    13981 tcaaaaaaca tatttataaa aaaattgacg gagtaacagg agatatatta ggctgtggaa
    14041 tagaactttc agagcttgta tatttaattt acatatattt gttaattttt atgttttttt
    14101 aaagtaaaaa gtggtgataa tatgataaga ttaatattaa taagacatgc actgacaaat
    14161 gataataaaa aaggaagatt atcaggccat ataaatagtt gtattagtga agagggcaaa
    14221 ctacaaataa ataaaattac cagatatctg agtaatgaaa acatagataa ggtttataca
    14281 acaccatcta cacgtactaa agatacagta gaaaaaatat caaaattgaa attattagaa
    14341 atagaagaaa aagaagcttt aagagaaatt agttttggag actttgaagg aagaactttt
    14401 gaagaaatta aaatcaaata tccaaatgag tttgaaaaaa tgataaaaga aggtaacaac
    14461 tatagatatc caaatggaga aagtttgata gactcttata aaagagtggc aaaagaaatt
    14521 gataatataa ttttagaaaa taatagtaat ttagatacaa agacaatact tatatgttct
    14581 catgcaggaa caataagaaa tataataaca catttgattt ctggtagtta taagtatcac
    14641 tggaacttta aaattgataa tgcatctatt acagttttag aaattgatgg tggatttgct
    14701 gtaatagata aaatgaattt tacagacttc atataattta atcaaaacca aacattacaa
    14761 tctgtgtttg taatatatct gtaaaattag caatgaaaat agaatattag attaaaatta
    14821 ggttcttaat tagatgttaa aagggaaaaa ggttagatgc ctttgcagcc cccgctactg
    14881 tgaaaccaac gaaactataa ataccactgt cagtttgatg ggaaggttat agaagtagga
    14941 tgaagttaag ccaggatacc tgcctaattt aatttaaaac aagaggtctt cggggtgaaa
    15001 gatttttaat taatatatat aaaagtccta tctctagaag tattaggact ttttttaatt
    15061 tgttttttag taaattttaa caaggaggga tggaaaatgg ggaaaaaaat aatgctacaa
    15121 ggaacagctt caaatgtagg taaaagtgtc ttagtagcag gtttgtgtag aatatttaag
    15181 caagatggtt atactgttgc tccttttaag tcacaaaata tggctttaaa ttcttttata
    15241 actaaagaag gattagaaat gggaagagca caagtatttc aagcagaggc tgcgaaaata
    15301 gagccaatag ctgatatgaa tccagtttta ttaaaacctt ctggaaataa taaaagtcaa
    15361 gttattgtaa gaggtaaagt tgtaggagaa atgccttctt ctaagtatca tgattataaa
    15421 ttggagttag tagatgtgtt gaaggaaaca tttaatagct taaattcaag gtatgatatt
    15481 gtagttatgg aaggggcagg aagtaatgca gagataaact taaaagatag agatatctct
    15541 aatatgggta tggcagaaat agctgatgca cctgtaataa tagtcggaga tattgataga
    15601 ggaggcgtat ttgcctcatt agcaggaaca atgcttttat taaatgaaga tgagaaaaga
    15661 agagtaaaag gtgtaataat caataaattc agaggaaaaa aagagctttt acaagatggt
    15721 gtaaagatgc tagaagacat cataaaagta cctgttatag gagtagtacc ttatacagat
    15781 ataaaaatag aagatgaaga cagtgtaact acgagattta agaaaaaaat gaataaaagt
    15841 gatatacaca tagaaataat tagaactcca catatgtcaa attttacaga ttttaatata
    15901 tttgagacac aagaagatgt aagtattaga tatgtagact atggagaatc tttaggaaat
    15961 ccagatatat taataatacc tggaactaaa agtactatag acgatttaac atatataaga
    16021 aaaagtggtc ttgaatcgca aatcaaaaat ttacatagtc aaggtaaatt gatatttggg
    16081 atatgtggtg gataccaaat gttaggtaaa aaattaaaag acccttatca tgttgaaagt
    16141 gaaattgaag aagttgatgg aataggtctt ttagatacag aaacaacgtt tgagaaagaa
    16201 aagactacaa cacaagtaga agctactatt gttgaatgta tcagtgaata catgaataac
    16261 cttaagggaa aaaaagtaaa aggttatgaa attcatatgg gaattacaaa gcgaggtaat
    16321 gatgtaagaa gtttagattt aataactaaa aaacttgata aagaagtgaa ttatactgag
    16381 ggaagtgtaa ataaagaggg gaatgtaatt gggacttatc ttcatggaat atttgatgaa
    16441 ataagcttta caagagcaat tttaaacaat ataagaaaga aaaaaggttt aggagaatta
    16501 gaaagcaaag taaactcttt taatgaattt aaggataaag agtatgataa gttggctgat
    16561 tttttaagag aacatttaga tatagagaaa atatatgaga ttatgaaata atgatgactt
    16621 ttatttataa tttaggtagg aggtgtatta tgaaaaaaat tttaattgcg ggtactaata
    16681 gtggagtagg taaaacaaca atatcattag gaataatgca agctttggtt aagagaaata
    16741 tgaaagtaca gccatataaa gtagggccag actatataga cccttcatat cacactttta
    16801 taactggaag acattctaga aatttagatt cttatatgtt agatgatgaa aaaataaagt
    16861 atatagtaaa taagtcctca aaagatgcag atatttcagt aatagaaggt gttatgggct
    16921 tatatgatgg ttttggaata gatttagata attgtacaag ttcacatact tcaaaggtat
    16981 taaagtcacc tgttatatta gttataaatg gtaaatctat ggcagcatcc agtgctgcta
    17041 tggtacttgg atataaggaa ctagataaag atgtaaatat aaagggtgtt atagtaaata
    17101 atgtaaagac taatagccat tatcaaataa ttaagagttc tatagaaaaa tattgtaatg
    17161 ttgaagtatt ggggtatttt cctccaaata acaaattctc tttggaatct cgacatctag
    17221 gattagttcc tagtgtagaa atgaaatcac taagggaaaa attttatacc ttagcagatg
    17281 aaattgaaca ctatatagat attgacagaa taattgaaat atcagaaagc gatgaatttg
    17341 agagtgattt tgattttcaa actttgatag aaaataataa aataaaagaa catgttaata
    17401 ataaatcaat agctatagca tatgataaag cattcaattt ttattataga gaaaatatag
    17461 agcttttaga ggaacttggt ttagaaataa actattttag ccctcttgaa gatgcatcag
    17521 tgccaaatgc agattatatt tatattggag gaggttttcc agaaatattt ggaaaagaat
    17581 tagaagcaaa tgagtcaatg agaacttcca tattagaagc acataacaat aatataccta
    17641 tttatgcaga atgtggagga ctgatgtacc taggtgagaa actattgaat caagaagaac
    17701 aagaatttaa tatggttgga atatttagtg gaattagtaa gatgacatct tctttaaaga
    17761 gatttgggta ctgtattggt gtagccaagg aaaatactct tttatctaaa aaaggggaat
    17821 ctattaaagg gcatgagttt catcattcaa tatttgaaag tgatgaaaaa tgtgtatatt
    17881 caatgagaaa agaaagagat ggaaatatag tagaagagtg ggatggtggt tatagtaaag
    17941 gaaacacttt ggctacatat cttcatactc atttttataa taacttaaat tgtatagaaa
    18001 attttttgaa aaaaggaaat gaataaatga gtattttatc tatttatata ggatatgtga
    18061 cagacttaat tgtaggagac ccatattctt ttcctcatcc agtgagattt attggtaaac
    18121 ttataaattt tactcaaggt ataataagaa aggtctctaa aaatgatagg cagttaaaat
    18181 ttgggggatt tatattatgg tttataatcg ttggaataac atatttaaca acttatgtta
    18241 tagtgaagtt atttagtttt aatatattat tgagtattgt agtgaatagt tttataattt
    18301 atacaacatt agccacaaaa tgtttaaaag acgaagcatt aaaaatttat aatgtattaa
    18361 aaactgaaga tatagaaaaa tctaggattc agctatctta tatagtaggt agggatacta
    18421 ctaatttaag tgaacctgaa ataataagag caactgtaga aactgttgca gaaaatactg
    18481 tagatggtat aattgcgcct atgttttatg catttattgg aggagcacca cttgctatgg
    18541 cctataaagc tataaataca ttggactcaa cagttggata taaaaataaa aagtataaag
    18601 atattggttt tgcatctgct aagattgatg atatagccaa ttatatacca gctagaatat
    18661 ctgtcattct tatgacaatt ggaagtttct ttttaaatta taactatagg aactgtttta
    18721 aaatctctat tagagataga aagaatcata aaagtccaaa ttgtgctttt tcagaaggtg
    18781 cagtatctgg tgctctagga atacaactag gaggtaccaa cgtttatttt ggggaaaaag
    18841 tatataagcc aacaataggg gataagctta gggagataga taaagaagat ataattaaaa
    18901 ctaataaaat tatgtatgca tcttcatttg tatctatgtt ggtatttaca ctaatatatg
    18961 tgttatgtag gatgatttta attaacttaa ttatataaat tattgtttgt taacttaagg
    19021 gggacataga tgaaggattt agggcatggt gccaatgtag atgaaatggc aagattatat
    19081 gggaaaaacc caaaagaaat aattgatttt agttcaaaca taaatccaaa tgtattacca
    19141 aatttagaga gatacatttt aaaaggctta gaagagtgta ggaattatcc agatataaat
    19201 tatacaagct tgagagaaaa tatatcaaaa tatatagata taaatccaga ctttataata
    19261 cctggcaatg gtgcaacaga agtgatttat ttgcttatga agagtattaa aaaaaaattg
    19321 gctataataa atccgacttt ttcagagtat agaagaagtg ctgagttaaa taatttagat
    19381 attatagatt tagagttaga tttagaaaat aactttaaac ttaatataga tataataaaa
    19441 gaaaatataa aaagatttga tagtttattt atatgtaatc ctaataatcc gagtggtaat
    19501 gtccaagatt taaaggaact tgttcattta ctagataaac ataataaggt actaataatt
    19561 gatgaaactt tcatggaatt tgttgaagat gaaagtaaat atagtttggt taaatatata
    19621 gaatcaaata aaaatatatt tataattaaa gctgtgacta agttttttgg tatgcctggt
    19681 ttaaggctag gatatggtct tacaagcaac actgaaatta tgaacaaaat ttatgaacac
    19741 aaagagccat ggactatcaa ttcttttgca gatatactat ccaattttat ttttgaagat
    19801 aaagagtata ttaaaaatag taaagaatat tacatagaag aaagaaaata tatgttacaa
    19861 gagttgagaa acataagaaa tataaaggtc tatgatacag atgctaattt tatcttgata
    19921 aaaatatata aaaaaacaac taaagaattg aaaaaagatt tgtttaagca aggaaacata
    19981 ttagttagag atgcgtctaa ttttatagga ttagatgata gttttataag agtggctata
    20041 aaatctcatg aagataacaa aattcttata gaaaatatga aaaatttatt gggtgattaa
    20101 aatgaaatct tatggaatat gcccagcatc ttgtggagaa tttgtacaag gtataattga
    20161 tgatgaagaa tatttatgtt catatgcaat agatatgtat tctaaagtgt atattgaaga
    20221 aaaattagta gatataaatt tagggagata taagtcaagg ttagcaattg aaaaggtatt
    20281 tgaaaaattt aatttaccta aaaaatatac taaaaatatt tctttaaata taaatagtaa
    20341 aatacctatt ggaaaaggaa tggcaagttc aactgctgat ataggagcta ctataaaggc
    20401 aacattatca ttgatagata aagatttaag tagtgaggag atttctaaat tagcagcaga
    20461 aatagaacca acagattcta tatttataga taaaaatagt atatttaatc ctctaaatgg
    20521 aactgttata aaatacctag gaaatctgac taatgcgaaa gttgtaatac ttgaaccaaa
    20581 caaagtacta gatactatga aaattagatt gaggcaagat tataataaat taaaagtaga
    20641 aaataaagaa gtaataaaaa aatcatttgc tcttttagaa gaaggattga aaaaaaataa
    20701 tttatctcta gttggagagg cttgtacttt gagtagctta gcaaatgaga atatagaaaa
    20761 aaaagagtat ttaaatgaaa taataaaaat atcaaaaaaa tatggagcat atggagtaaa
    20821 tattgctcac agtggaactg tagtaggtat tttaattgat aagtttatga atgataaaaa
    20881 gatgatagat gccttatgtg aatctaacat aaattctgtc tataataaaa tttatacaca
    20941 aaacataatt aatggaggaa ttaaggggga aatagaatgg aatacataaa gaatcctatg
    21001 aaaatagaag aaaaaagttt tgaattgata caagaaataa tagacgaaat tagaccagat
    21061 tataaattta agaataatat cgaagaaaaa ataataaaaa gagctataca tactactgct
    21121 gattttgatt acttagatat attaaaaata tcagaagaag cagttgatag tataatagat
    21181 gcattaaaaa ataatgcaag tatttatact gatacaaata tggctcttag tggtataaat
    21241 aaaagaaagt tagaatcatt aggatgtaag tataaatgtt tagtggcaga tgatgaaact
    21301 gtaaaattag ctaaagaaaa aggaattact cgttctatgg cagcagtaga aattgcagct
    21361 aaagaagaag gaagaaaaat attcgtttta ggtaatgctc caacagcttt atataaagtt
    21421 atggagatga aatcagaagg tagattagat ttagatgcag ttataggagt tcctgtaggt
    21481 tttgtaggag cacaagaatc aaaagatgaa gttgaaaaaa cagatatacc atatataatt
    21541 tctaaaggta gaaaaggtgg cagtaattta gcagcagcaa tagtaaatgc tatattatat
    21601 actatgtagg tatctatatg gaagaatatg tatatataga tggaaaaaaa tatagaagag
    21661 gatatactac aggttcatgt gcaacaggtg catctaaggc tgctgtatat atgttgatta
    21721 caaaaaatag aatcaatact ataaatatag atactcctaa ggggatacca ttactactaa
    21781 aagttgataa tataaatatt tctgatacat ttgtggaatg ttccataaaa aaagatggtg
    21841 gagatgatat agatgcaact cataccatgg atatttatgc aagagctgaa attgtagcta
    21901 aaaatgataa gaacaaaggg tatcttactc taaaggatat agatagttta agcaccaata
    21961 gtgagtgtaa aagtgagctc tataagttta ttagggttta tggcggaact ggtattggtg
    22021 tggttacgaa aaaaggtctt agcgtggatg ttggtaaacc tgctataaat ccgactccac
    22081 ttaaaatgat aaatcatgaa ataagaaagc ttataggaga taattttgaa tctatacttg
    22141 gaaatgataa agttttaaaa ataacaatct ttgctccaca aggagaaact gtagcaaaaa
    22201 aaacatttaa tccaagatta ggtatagttg gaggaatatc tataataggt acaactggga
    22261 ttgttgaacc tatgagtgat gagggttgga agaagtctct atctatagaa cttcagatga
    22321 aaaaagaaca aggcttagat aaaataatat tggttccagg aaatcatgga gaacagttta
    22381 taagagaaaa attaaactta gatattaagt atgtagttag ggtaagtaat tttataggct
    22441 atatgattaa agaagctcaa agaataggat ataaaaaaat cttaatggca ggtcatatag
    22501 gtaaatttat aaaagtatct gctggtattt ttaatactca tagtaaggta gcagatgcta
    22561 gaagtgagat attagtggca aacttagctt taatgggagc tagatacgaa tttttaaata
    22621 aaataaatca atgcgtaact actgaagaag ctgtagagct aataaataat agtgaatata
    22681 gagaagtata taatatatta agtaataaat gtagggagag agtaaagcaa tacttgaatg
    22741 aagattcaga tgatattgat gtggaagtaa tcatattttc aatggataag tctttattag
    22801 gtaaatcgga taatacagat gatttagtgg aggtctttat atgataaaca taataggtct
    22861 aggacctggt aacttagact atataacaaa aaaaggtgaa aatttaattt ccacttctga
    22921 tgtattaatt ggtggtaaaa gaaatttaga gtctataaaa aattttgaag gagaaaaaat
    22981 agttctggat tctaatttaa gagaaattat agagtacata aataacaata aagaaaagca
    23041 aatatcaata atagcatctg gggacccatt aatatatggg ataggtagat atctatctaa
    23101 aaatattgat aataagatgt taaacatggt ttctgggatt agttcattac agtacatatt
    23161 ttcaaaaata tatgtagata tgaatgatgt atatattaca agtagtcatg gcaaagtacc
    23221 agattttgat tacatattat cacataaaaa agtatgtatg gttacagatt caaagatagg
    23281 accaaaacaa ataagtagag aaataataga tagaaatcta aataaaatta ttgttgtagg
    23341 agaaaatctg tcatatgaca atgaaaaaat aacaatagca aaaccagaag agataataag
    23401 aatagacaat tttgatatga atgttgtggt gattttagat gaggaatagt gaatttataa
    23461 caggtaaagt tccaattact aaggaagagg ttagagcaat atcaataagt aagttaaatt
    23521 tagtaaatgc taaaaacctc atagacatag gggctggtac aggaagtgta agtgttgaag
    23581 ctgcatataa ttatcctaat ttaaaggtta tatctataga gaaaaatgat gatgctatag
    23641 agcttataga gaaaaatata aaaaaattca acttaaaaaa tatagaagtg ataaaaggat
    23701 gtgctccaat taatctaggg ttaaatgtta aagttgagtc tatttttctt gggggaacag
    23761 gaaataatct ggaagaaata atagaatggt ctaaaaaaaa tcttgttaca ggaggaagac
    23821 ttgtagctaa ttttataata atagatacat ttaatcaaac tttaaaactg cttaaaaagc
    23881 atggatttaa agaaatagat gtatgtgttt tgaatgtttc taaactggag aaactaggaa
    23941 aaggtgaata ttttaaacca ctaaatccta tatatataat atcttgtgaa aaaggggaaa
    24001 atgatgatga ataaagtaca ttttgtagga gcaggacctg gagataaaga gctgataaca
    24061 ttaaaaggat acaaattatt aagtaatgca gatgtagtga tatatgcagg ttcacttgtt
    24121 aatcctgaac ttttggaata ctgtaaagaa gattgtcaaa tacataatag tgcatatatg
    24181 gatttacaag agataataga tgttatgaga gaaggtatag aaaataataa atctgttgta
    24241 agactgcaaa caggtgattt ttctatatat ggttcaatta gagaacaagt ggaagattta
    24301 aataaattga atatagatta tgactgtact ccaggagtaa gttctttttt aggggcagca
    24361 tcatctttag gggttgaata tacagttcca gaaatttctc aaagtgtaat tataacaaga
    24421 atggaaggca gaactccggt tccagagaaa gaatcaatac aatcatatgc taagcatcag
    24481 acatctatgg taatattttt atctgttcaa gaaattgaga aagttgtgtc taaattactg
    24541 gaaggtggat atccaaaaga tactcctata gcagttatat acaaagcaac ttgggcagat
    24601 gaaaaaatag ttaaaggaac tttaagtgat atagctgtaa aagttaaaga aaacaacata
    24661 aataagactg ctttaataat ggttgggaga tttttaggag aagagtataa taattcaaag
    24721 ttatatgata aggatttcaa acatgaatat agagggtaat attgctataa tctgtattac
    24781 agaaaatgga aaaaaattag ctgtaaagat aaattctcta gtaaataact gtgttatata
    24841 tcaagtaaaa aataaaaaaa gtaatttagt tgttgaagaa acatgtgtac atacagttca
    24901 aaagaagtta agtgattttg taggagaaat atttagcaaa tttgattaca tagtatttat
    24961 aatggctaca ggtatagttg tacgaagtat agcaccatac ataataagta agtttagtga
    25021 ccctgcaatt ttggtaatgg atgaaaaagg caaaaacgta ataagtttat tgagtggtca
    25081 tattggaggg gcaaacagct tgacacagta cataagtagt cttattgatg ctaatccagt
    25141 aataacaaca gctactgata taaatcaaaa agcttcctta gatatgatag caaaaaagct
    25201 aaatgcacat attgataatt ttagagaaag tgtaaaagaa gtaaattcta tgctagtgaa
    25261 taataaatgt gttggattat atgttgatga aggatatcaa gttgatacta ggggatttaa
    25321 acttatagaa agtaaggata agttaagaga tagtttttca aacacatata tagaaaaatt
    25381 agttgtagta agcaataaaa taaattgtat tgaagatatt gagaatttaa acctagataa
    25441 taaatttgaa cattgctata gtaccaaaaa aataataaag gtaattccaa gagatatagt
    25501 gataggaatt ggctgtagaa gaaacacaga tagtgagctg atgtataatt cacttgaaga
    25561 gtttcttaat aaacaaaata tagatattag agcaataaac acagttggaa gtatagaact
    25621 gaaaaaagac gaaaaagcaa taattgaatt atcaaaaata ttgaaggtgc catttaaaac
    25681 attttcagta gatgaaatat ctaaggttga taatcttttt gataaatctg attttgttaa
    25741 aaaaaatgtg ggcgtgtatt cagtagcaga acctgtagca tatcttttga gcaaaggtaa
    25801 tttgttagtt gaaaaacata aatataaagg aataactttt tcagtgggga gaataaaaat
    25861 atgatatacg ttgtaggaat tggaccagga agtaaagata caatgacttt agaagcaata
    25921 aaagcaatag aagattctga ggctattgtt ggatataaga cttatataaa actaatagaa
    25981 gaatttatac aaggtaaaga aataatccaa aatggtatga ggcaagaagt tgatagatgt
    26041 aaacaagcag tagaggaagc aaaaaaagga aaaaaagtag cagttgtaag cagtggtgat
    26101 gctggaatat atgggatggc aggtcttatt ttagaattaa tatctaaaga agatgaagat
    26161 ataaaagtaa aggtaattcc aggtgttact gcaagtattg gtgcagcagc tatacttgga
    26221 gctcctatta tgcatgattt ctgtcatata agtttgagtg atttaatgac tccgtgggag
    26281 gtaattgaaa aaagattgag attggctgct gaagccgatt ttgtaatttg tttgtataat
    26341 ccaagaagta agggaagaag tgaacatcta agcaaagcat ttaaaataat gggtgagttt
    26401 aaaggtggag atacacctgt tggagtggta aaagatgtag gtagagaaaa agaagaaaag
    26461 tttgtatgta cttttgaaaa tatggacttt gaaaaagtcg atatgactac aatggttata
    26521 ataggaaata agtctaccta tattaacggt gaattgatga taactccaag aggttataca
    26581 gtatgatttt aattttagga ggaacttcag atagtttatc tatatgtgat aagataaatg
    26641 aactgaaaaa tcaaccatat atcctttcag ttactacaga ttatggaagg gaacttgcac
    26701 aacaacatgc agagaatgta attttaggta agcttggaaa agaagatatg ctaaaattta
    26761 taaaagataa taatatagta aaaataatag atgccactca tccttatgca gttgaagtat
    26821 ccaaaactgc tattacttgt gcaaaattat tgaatataga ttatataagg tttgagagaa
    26881 aatctttaat tgaagaaata gattatgata ataaatttat agtagataca attgaagaag
    26941 cctgtgaaat agcaaataaa atagggacaa atatattcat aggaacagga agtaaaaatt
    27001 tgaacagatt tatagaggaa ataagtgata aaaatttagt tgttagagtt cttccaacga
    27061 gtgaagtcat attaagttgt gaaaaattag gtttaagtgc agataatata gtagctatga
    27121 aaggtccatt tagccagtat ataaatgaag aaacatataa acattacaat atagatttag
    27181 tcataacaaa agaaagtgga atagctggtg ggttcttaga gaaggtaaat gcatgtaaaa
    27241 ctttcaatat accagtaata attataaaaa gagaatcgat taactacgaa aatgtattta
    27301 atgacataaa taaaattaaa gacatacttg tgtaattttt aggaggagaa tatatgaaga
    27361 aagccttatt agtagtgagt tttggtacaa gctatcatga aacgagagaa aagacaatag
    27421 atatatgtga agataaaata aaaaattcgt taaaaagcca tgattttttt agagcttata
    27481 catcaaatat gataataaat aaaataaaaa aaagggatgg aatagaaata gataatccaa
    27541 tacaggcttt agataaaata tatgaacaag gatatgatga agtaataatc caaacacttc
    27601 atattatatg tggcgaagaa tttaacaaat taaaagaaca agtagatgga tattcttcta
    27661 aattcaaaaa gattgtatta ggaagacctt tgcttacaca tattgaagat tatcaggaag
    27721 ctgtagaggc aattaagtat caaattcctc atatggaaag taatgaagca gtagtattta
    27781 tgggacatgg aacacttcat gaatcacatt cagcgtatcc agcattggaa tacatgctta
    27841 gggataatgg aataaatgct tatgttggta cagttgaagg gtatccagaa atagaacatg
    27901 taataagaag attaaaagaa ggaaatataa aaactgtaaa tttgatgcca tttatgttag
    27961 ttgcaggaga ccatgctata aatgatatgg caggagatga agaagattct tggaaaacaa
    28021 tattagaaga gaatggattt gacgtaagag ttcatttaaa aggactagga gaaaattctt
    28081 atatacaaga taaatttgtt cgtcatgctg taaaatgtgc agagaatgcg aagttctatg
    28141 gtataggagc agggccagga gatggttctt tacttacgat aaaagcggtt aatactttaa
    28201 agaaactgga tattctatac actccagaat caaaaaaggg tggagatagt ctagctttat
    28261 caatagttag tgaatattta cctgaatcag tggaaataaa gtctaggcat ttcccaatga
    28321 gttttgatgg gaatgaaaaa agtttagctt gggacaatgt ggcacaagaa attatatcag
    28381 atgttaatga tggtaaaaat gtaggatttg ttacattagg agatcctatg atttatagta
    28441 catatgtata tataatgaaa agaatattag gagaggtaga agtcgaaact ataccaggaa
    28501 tttcatcatt ttccaatata gcatctaatc agaattttcc attagtcatg gataaagaag
    28561 ctttagtggt aattccttgt actatggaag atgataagat agagtatgct ttgcaaaatt
    28621 ataattcaat agtattgatg aaggtatata aaaattttaa agaaataata aaaaaattag
    28681 aaaagtatga tttaatagaa catgctattt tagtaagtaa ttcttcccaa gaaagcgaaa
    28741 atgtgtttac agatttaaaa gaagcgcacc ttgaagataa gatatcttat ttttctacta
    28801 tactagtaaa taaagataat aaaataaaat aggtgaaagc aatatttaaa ggagacttaa
    28861 tatgaatata gtggttggaa ctaggggtag taatctagcg cttatacaaa cagaatgggt
    28921 aataaatgaa ttaaaaaaga agtatccaga aattagtttt gagattaaaa tcataaagac
    28981 aaaaggagat ttaattcaaa atgtttcact ggataaaata ggtgataaag gattgtttgt
    29041 caaagaaata gagcaacaat tattagatgg aaaaatagac atagcagtac atagtatgaa
    29101 agatatgcct tcttatttag cagatggact aaaatttgct catacgccta agagagaaga
    29161 cccaagagat gttctaatac ttagagaagg atataaaaat ttagatgatt tgcctcatgg
    29221 agcagtaata ggaacaggaa gtaaaagaag gaagtttcaa ttattgaaac aaagacctga
    29281 tttaaatata gtacaagtta gaggtaatgt ggaaactagg atacgaaaaa taaaagatga
    29341 aaacatgcat ggtattgtgt tggcagcatc aggaataatt agagctaatt tacaagataa
    29401 aataagttct tatttacctg ttgatgttgt tattccagca cctgcccaag gagctttagc
    29461 tatagaaatt agaagcaatg atagtacgat agagggaata gtaaattcac tgaaggatga
    29521 gaatacagaa atccaaatat tggctgaaag aggtttttta gatggtgtaa atggaagttg
    29581 tcatattcca atggcagctt attgtgaaat aatccaagat aaaatacatc ttactggctt
    29641 atatggagat tcagaaggaa aaaaagtagt aatcaagtct atagatggag atatttcttc
    29701 tcctagagag ttaggtttaa agttagctaa acttgtattg aaggagtatg agaattatga
    29761 agggtaaggt ttacttagta ggggcaggac caggtgatta taaactcatg actttaaaag
    29821 gtttagagtg tataagaaaa tcggatgtaa tagtatatga tagattagca aatagtagtt
    29881 atttaagaga agctaagcct gattgtgaac ttatatatgt aggcaaggca tcaagtaatc
    29941 atattttgcc acaagaagat ataaatagaa ttatagcaga aaaagctaaa gaaggaaaaa
    30001 ttgtaactcg tcttaaagga ggagaccctt atgtatttgg cagaggggga gaagaagcag
    30061 agactttagt agatgaaggt atagaatttg aagtagttcc aggtataact tctgctatag
    30121 gaggtttatg ttatgcgggt attcctataa cacatagaga ccatgcttcg tcatttcatg
    30181 taataacagg tcatttgaag ggtgatgata gtggagaatt aaactggaat gctcttgcaa
    30241 ataacaaagg tactcttgtg tttttgatgg gaatttcaaa cctagagaaa ataagtgaaa
    30301 acttaatgaa agaaggaaaa gataaggata cacctgttgc tcttataagt tgggcaacta
    30361 gatataatca aagagttgtt acatctacac ttgaaaatgt ttatgaaact gctattaaag
    30421 aagaagtaaa accgccaact cttatagtag ttgggagtgt tgtaggactt agagaaaagt
    30481 tgaacttctt tgaaagtaag cctttatttg gaaaaagtat tgctgtaact aggtctagaa
    30541 atcaaaatag tgtattggta gaaaaaataa tggatttagg tggaaatcct atagaaatac
    30601 ctactattaa ggtagaaaaa atacaaaata atattaaatt agaaaatgaa ataaaaaata
    30661 taaataagta taattatcta atactaacta gtaaaaatgc cgtagaaatc ttctttgaaa
    30721 agatatatga aatgaatttt gatttaagga tattatctaa tttaaaggta tgtgctatag
    30781 gttctgctac atcaaatgag ttgaaaaaaa gaggaattat agcggatata gtaccaaaaa
    30841 aatttgtagc agagtcactg tatgaagagt tagcgcctat tttaaatgat gacgataata
    30901 tacttatacc tagagcagaa aatgctagag attatctggt tgacaaactt aaggctattt
    30961 gtgaagtaac agaagtacat atatatagaa cagtaataga tgaggagaaa aaaggtgaga
    31021 tattagatat tttaaattct aatgacatag attatataac atttactagt tcctcaacag
    31081 taaataatct tgttgaaatt ataggaaaag aaaatattaa aatattagaa aaaataaaaa
    31141 cactatctat aggtcctata actacagaga caataaatag tttgggaata aaattgtaca
    31201 aagaatctgc tacatctact atagattcgt tagtagattt aattataaaa aattaatttt
    31261 ggaggcttga atatgttaag aagaccaaga agactaagag caagtaaagc aatcagaaat
    31321 ttggtaagag aaactaaggt gaatgtagaa gacttaatat atccactgtt tgtagtagag
    31381 ggggaaaata taaaagaaga aattaaatct ttaccagatg tctatcattt atcaatagac
    31441 atgctagaag acgaaataaa agaaataagt gagttgggaa ttgaacatat aatgttattt
    31501 ggtgttccag atgaaaaaga tgagtgtgct tcacaagctt ttaatgataa tggaatagtt
    31561 caaaaggcaa ttagaaaggt aaaagaaata aattcagata taaatgtaat aacagatgtc
    31621 tgtatgtgcc aatatacaag tcatggacat tgtgggattt taactgatac tggttatgtt
    31681 gataatgata aatcattgga gtatttggct aaaatatctg taagccatgc taaagcagga
    31741 gctgatatgg ttgctccatc tgatatgatg gatggaagaa tacaagcaat aagagaagct
    31801 ttagatgaaa atggattaga aaatgttgga atcatggctt atagtgttaa gtatgcttca
    31861 aattattatg gaccttttag agaggcagct cattcagcac catcttttgg agatagaaaa
    31921 acatatcaaa tggacccagc aaatagcaat gaagctataa tagagactga acttgatata
    31981 ttggaaggtg cagatatact tatggttaaa cctgcattat catatttaga tattataaga
    32041 agagttaaag ataattttga cttacctcta gcagcttata atgtaagtgg agaatactct
    32101 atgcttaagt ttgctgtgaa ggaaggttta ttaaatgaag atgtaattct tgaatcaatt
    32161 acatctataa aaagagcagg agcagatata ataataactt attttgctaa agacattgct
    32221 aaactgttga aaaaataaat tctagtagta taaagaatat caaaaagtat aggaattgta
    32281 attaactatt atacagtagt tttgtttaga catttattga agaataatag aaactaattt
    32341 gcaatttcta tactttattt atgtaactaa ataaaatttg gaggtagaag atgctatatc
    32401 ctattaattt aaaattagat gagttagatg tagttataat tggtggtgga gaagttgcat
    32461 ataggaaatg taaaaatttt ttagagttta acaaaaatgt tactatagtt tctaaacaaa
    32521 ttttaaataa aatttatgat ttaaaaggaa atataaaaat aataaaagat gattataaag
    32581 aaatatatat aaaagatgct tctgtaataa ttgctgccac aaataataga gagttaaata
    32641 tggaaatagg attatattgt agagcaaaaa ataagctagt gaatgtagtt gataatatag
    32701 aaatatcgaa ctttactgtt ccatcatata taaaaagagg agatttactt ataagtgtgt
    32761 ctactggggg caagagtcct tcattatcat caaagataaa aaaagaaatt gaagagagat
    32821 atactgaaga ttatgaggag tatttaaatg tattaggaga tataagaaaa aaggttataa
    32881 aaaaatacga agataaaagt aaaagaaaag atgtgcttaa tatgttggta acacttgacc
    32941 tagaagaact taagaagttt gatatataaa aaataaaata aaaaatgtta aaaaggtgtt
    33001 gacggattat ttttaagatg atatagtatt acttgtcctt aagaaaaggg caaacataa
//
LOCUS       DAEMHW010000024        33026 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEMHW010000024 DAEMHW010000000
VERSION     DAEMHW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.024"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            116..256
                     /locus_tag="KPK67_000944"
     CDS             116..256
                     /locus_tag="KPK67_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457945.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(431..751)
                     /locus_tag="KPK67_000945"
     CDS             complement(431..751)
                     /locus_tag="KPK67_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG2457946.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(871..1608)
                     /locus_tag="KPK67_000946"
     CDS             complement(871..1608)
                     /locus_tag="KPK67_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBG2457947.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1823..3646
                     /gene="acd"
                     /locus_tag="KPK67_000947"
     CDS             1823..3646
                     /gene="acd"
                     /locus_tag="KPK67_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBG2457948.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3861..8159
                     /locus_tag="KPK67_000948"
     CDS             3861..8159
                     /locus_tag="KPK67_000948"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBG2457949.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8363..8824
                     /locus_tag="KPK67_000949"
     CDS             8363..8824
                     /locus_tag="KPK67_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBG2457950.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8854..10008
                     /gene="nusA"
                     /locus_tag="KPK67_000950"
     CDS             8854..10008
                     /gene="nusA"
                     /locus_tag="KPK67_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBG2457951.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10015..10296
                     /locus_tag="KPK67_000951"
     CDS             10015..10296
                     /locus_tag="KPK67_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBG2457952.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10286..10597
                     /locus_tag="KPK67_000952"
     CDS             10286..10597
                     /locus_tag="KPK67_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBG2457953.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10619..12559
                     /gene="infB"
                     /locus_tag="KPK67_000953"
     CDS             10619..12559
                     /gene="infB"
                     /locus_tag="KPK67_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBG2457954.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGAKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12602..12982
                     /gene="rbfA"
                     /locus_tag="KPK67_000954"
     CDS             12602..12982
                     /gene="rbfA"
                     /locus_tag="KPK67_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBG2457955.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            12963..13946
                     /locus_tag="KPK67_000955"
     CDS             12963..13946
                     /locus_tag="KPK67_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBG2457956.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEGVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSDYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            13959..14864
                     /gene="truB"
                     /locus_tag="KPK67_000956"
     CDS             13959..14864
                     /gene="truB"
                     /locus_tag="KPK67_000956"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBG2457957.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            14995..15924
                     /locus_tag="KPK67_000957"
     CDS             14995..15924
                     /locus_tag="KPK67_000957"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBG2457958.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16028..16285
                     /gene="rpsO"
                     /locus_tag="KPK67_000958"
     CDS             16028..16285
                     /gene="rpsO"
                     /locus_tag="KPK67_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBG2457959.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16525..17370
                     /locus_tag="KPK67_000959"
     CDS             16525..17370
                     /locus_tag="KPK67_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG2457960.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDVIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17495..19606
                     /gene="pnp"
                     /locus_tag="KPK67_000960"
     CDS             17495..19606
                     /gene="pnp"
                     /locus_tag="KPK67_000960"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBG2457961.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19872..20615
                     /locus_tag="KPK67_000961"
     CDS             19872..20615
                     /locus_tag="KPK67_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2457962.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            20773..22020
                     /locus_tag="KPK67_000962"
     CDS             20773..22020
                     /locus_tag="KPK67_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG2457963.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            22055..22300
                     /locus_tag="KPK67_000963"
     CDS             22055..22300
                     /locus_tag="KPK67_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG2457964.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22310..23533
                     /gene="dapG"
                     /locus_tag="KPK67_000964"
     CDS             22310..23533
                     /gene="dapG"
                     /locus_tag="KPK67_000964"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG2457965.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23646..24380
                     /locus_tag="KPK67_000965"
     CDS             23646..24380
                     /locus_tag="KPK67_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBG2457966.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24503..26914
                     /locus_tag="KPK67_000966"
     CDS             24503..26914
                     /locus_tag="KPK67_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBG2457967.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDDVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            26917..27975
                     /gene="mnmH"
                     /locus_tag="KPK67_000967"
     CDS             26917..27975
                     /gene="mnmH"
                     /locus_tag="KPK67_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBG2457968.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            27969..29303
                     /gene="rimO"
                     /locus_tag="KPK67_000968"
     CDS             27969..29303
                     /gene="rimO"
                     /locus_tag="KPK67_000968"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBG2457969.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29290..29832
                     /gene="pgsA"
                     /locus_tag="KPK67_000969"
     CDS             29290..29832
                     /gene="pgsA"
                     /locus_tag="KPK67_000969"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBG2457970.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            30120..31166
                     /gene="recA"
                     /locus_tag="KPK67_000970"
     CDS             30120..31166
                     /gene="recA"
                     /locus_tag="KPK67_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBG2457971.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            31342..32883
                     /gene="rny"
                     /locus_tag="KPK67_000971"
     CDS             31342..32883
                     /gene="rny"
                     /locus_tag="KPK67_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBG2457972.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT        13365 a         3504 c         6332 g         9825 t
ORIGIN      
        1 tatgtcaaaa aataaaaaaa tttttattta ttagagtttt tggaaataat aataacagat
       61 attataatta attaacagtt tttgtaagta tttgacaata gagagggtgt aacaaatggg
      121 aataatagag tcagcatcaa aattggctga gatggttcat ttattggctg ttgaaaaggg
      181 gataactgat atagaagcat gggatgaagc tgtgaaagaa tacagcaaaa tttatgaaga
      241 aagaagaaat gaatagaatg ataaaaaatg aaaacttgat gataaaaaat aactttattt
      301 aaagaaattc aatttttata gaagtattaa aaataatata aataaaaaga tggtatttca
      361 aatgtaagta aaaaatcatt ttgggatacc atccttatta tttattttaa taatatataa
      421 gtaataaatc ttacttatcc atatttttta tgtaacctct tttttctagc ttttttatat
      481 gctccttacg tatttcatca agattaatac catattgttc ttctagctta aacatcatag
      541 taacacatgt ctgcgctaca tccattagtt ccttagcagt tttctttata atatcaactt
      601 catctaaatc attattttct ccacttagat ttctgaattt acctatacat tcagctaatt
      661 caccttgttc ttcacccatt ttgataaatg tagattctag tgttgggtct aaattattaa
      721 gctctggtaa agatatagtt tttagtttca taaaagctcc tttcaagtta ttcgtattta
      781 tactaaaaca tcataacaaa aataatacta aaaaaaaact ttaaattcaa cataaaaact
      841 tacatatata ataaaaatcg tgaaataaat ctatatttgt cttaatacag cctcatataa
      901 aacttctcca ataggtttat ttattacaag agaagctgac ttatcataga ctgtatttgc
      961 tttattaata agagcaatat aatcaccttt gtaataatct ataaaacttg ctgctgggta
     1021 cacagctaat gatgtacctc ctataatcaa taaatcagca ttacttatag cagaaattgt
     1081 tttagtaatg acatcactgt caagagcttc ctcataaagt actacatctg gttttactat
     1141 agaaccacag ttgtcacagt aaggaatatt acctcccaga tttaacatag attctaagtc
     1201 aaaaaactta ccacatttag tacaataatt tctatgtaca gaaccatgaa gttctagcac
     1261 atttttactt ccagccattt gatgaagtcc atcaatattt tgagttatga cagctttaag
     1321 cttacccatt tcttcgagtt ttgctaaagc tatatgagca ttattgggtt ttgcattagg
     1381 atatattaat ttatccttat aaaattcaaa aaattcttct ggatatctaa caaaaaaagt
     1441 atgtgaaact aattgttcag cagtaaattg tttattgagt ttttggctaa ataatccagt
     1501 agaacttcta aagtctggaa tattagattc agttgaaaca cctgctccac caaaaaatac
     1561 aatattattg tgattagcta ttaagtcttt taagctattt gcatccatag ttacacctcc
     1621 aaattattat atataaattg tactacatat taggtctttt atgttttatt aatttaaata
     1681 aatatgtagt ttaataagtt aaataaaggg aatataaata aaatgtatac agagtataaa
     1741 tagacaatat ttactaaaat aacaagttgt cgtataatta tattaaaatt gttttagtgt
     1801 ataagaaggg cgggggaagt acgtgaagaa aaaagcagct ttagcaacat tggctatgtt
     1861 acccttaggt gtggtaaatg cacatgctga tggagatata ggtatagtga ctataaatta
     1921 tttaaatgta agaaatgaac caactgccga aagtagcata gcctttgttg ctaaaaaaga
     1981 tgataaagtt ttgattaaag actcttctaa tggatggtat aaaataaaag ctgaatctgg
     2041 acaagaaggt tgggcttcat caaaatatat agcaaaatca aatagtgact ctttaagaac
     2101 gtctacaaat aaagaaaaac aagttatttc aaatagttta aatatgagaa atggagcagg
     2161 tacaagttac agagtaataa ctgtactaaa aaaaggtcaa aaagtagaag taatatcaga
     2221 gagtaatgga tggtctaaaa tcaaatatga tggaagacta ggatatgtat ctagctctta
     2281 tttaggagac gtttcaaact caactaataa atcaaaaact aaacaagtga atacaacttc
     2341 actaaatgta aggagtggac caaatacaag ttatggtttg ttaggcaagt tgccaaaagg
     2401 aagtaaagta gaagtaatat cagaaagtaa tggatggtcc aaaataaagt ataatggaaa
     2461 agatgcctat gtatctagta tgtacttatc ggatgtaagt caaagtaatt cagacaattc
     2521 tagtcaaagc aatgataaaa agaatactga taaggttgta aatacagctt ctttaaatgt
     2581 aagaagtgga ccaggttcta catatagtaa gttgggaaaa gtttataaag gaagtaaagt
     2641 aactgtacta tcagaaagta gtggatgggc taagattaat tttaacaata aagaagcatt
     2701 tgtagtaggc aattatttat ctacttcagc agatacttca aataataact caaatagtaa
     2761 ctctgataac agttctaata gtaatggcaa taattcttca tcatcaggtc aagtaaatgg
     2821 tatgtcaggt ataagtgggg ctaaaattga ttataaatct ttgagttata ctttagaatc
     2881 tcatataagt aaacaagttg agaaagcagc atcaggagga aatgtgatag ctccaagtaa
     2941 taggaaaagt actccaagtc ctgaatttag tactttttca gctcaaagaa caagctcatt
     3001 tgtaaatgca agttcaagtg atatagaata ttatttaaat cctaagaatt ttacaaatac
     3061 tactaaaggt atgatgcagt ttttaaagat taacagttat agagatggta tttcagaatc
     3121 aagtcttaac tcgtatctta atggtttatc ttctagtgta tttaaaaacc agggagcagc
     3181 ctttataaat gctgccaaga agtataatat agatgttgta tatctagtat ctcatgctat
     3241 gtgggaaact gcttatggta agtctacact tgcccaaggt caaacattaa catcttataa
     3301 gggacaacct cttagtaagc cagttaaagt atacaatttt tttggtatag gagcaataga
     3361 taaaagtgct aatgtttcag gtgcagaagc agcgtattcc aatggctgga ctagtgtaga
     3421 agctacaata gatggctctg caaagtggat atcacaaaat tacgtaaata gttctaagta
     3481 caatcagaat actatctaca agatgaagtg gaattatgat tatacatggc atcagtatgc
     3541 aactgatgta aactgggcta atggaatttc tggaattatg gaaaatttaa ttggtcttta
     3601 tggaggagga agtagtttgg tttttgaagt tcctcaatat aagtaataca taaataaaaa
     3661 atatagacta tgggtagttt tcattttatt taaattatct atagtctttt tgtatataat
     3721 ttataatctg taaatttaat gttaacaaaa cttttatgtt ataatattaa ttctaagtaa
     3781 atataagtga gtaataggta gaaatagata aaaagtggta ataatatata taaagataat
     3841 tttgagaaag gaggatatat atggagagta ttaaggaata tttggataag ttagaaatta
     3901 ataatagtgg tcttggaaag caacttaaag aagtttatat aaatagagta gtttatttta
     3961 aggaagataa gatagtttat ttttatctaa cttctaaaga tattgtttct catgaacttt
     4021 tagataaatt taaagaagaa ttgatgtata aactcgatta ttttaaagat atgaggatga
     4081 agataagatt tactggtctt gaaagaaaat ccaataaaga tgttataaaa aaatattgga
     4141 ataatatatt gtatatatta aagtatcttt gtccatctat tgcagggtgg tataaacaag
     4201 tagaatttct ttgtttagaa gaagaattaa aaataaaact tccaaaaggt atattttatg
     4261 agcgattaat gaaaaaaaat gtaacttatg tacttaaaac cgtgcttagc gaagagttgg
     4321 gattagattt aaatataaca atagaaaaag ctgttgatga aaaggtaaat aaagaaagac
     4381 tcataagaat caatgataga gaaatggaag aaaaaattag ggctttagaa attggaaaag
     4441 ttaataactg tgaagaaaat gaagaggaaa gctatgtcat taagtctgat gtagatgaaa
     4501 atttaatcta tggagataat gcaaatgcta tgattgaaaa tatagttgaa ttaaatgcat
     4561 cttctggaac agttgcagtt gttggagata tatttgatgt tgacactaaa gaacttaaaa
     4621 acggaaaaat acttatgata gcttcaatta cagattatac tagctcaata agctgtaaac
     4681 tttttttaac agatacaaat aaggatggag tacttgaaaa tgttaaaaaa ggagcatact
     4741 taaaaatcaa gggtgacata gtttatgata catatcaaag agaaattagt atgatgatta
     4801 gtgggataag aaaagaaact agaatagaga gagaagataa atcagaggaa aaaagagtag
     4861 aactccatgc acatacacaa atgtcttcta tggatgctat ttgttctgtt aaaaagcttg
     4921 tagagcgtgc agccaaatgg ggtcattctg ctattgctat aacagaccat ggtgttgtac
     4981 aaggatttcc agatgctatg aatgcaggta agtcaaataa tataaaaata ctttatggag
     5041 tagaagggta cttggttgaa gatgattctc tcattataga ggatgcaaat gataaagaac
     5101 tttcgcagac atttgttgta tttgacatag agacaactgg cttttctaat acaaatgata
     5161 aaataactga gattggtgct gtaaaaatag aaaactttaa gattgtagat agatttagtg
     5221 agttaataaa tcctgaaaaa gatatttctt ataagataca ggaattgaca ggaataacta
     5281 atgaattaat taaagacaag ccaaccattg aagaagttct tcctaagttt atggaatttg
     5341 ttggagatag tgtattagta gcacataatg cagagtttga tactggattt atatcgcaaa
     5401 aatgtaggga acaaggcttg ttatataata acaaaaaagt agatacactt atgttagcaa
     5461 gagtaatgtt gccaaactta aaaagacata gattaaatgt cgttgcaaaa gaacttggta
     5521 ttcctctttt aaatcaccat agagcagtgg atgatgctga agctacagca cttatattta
     5581 ataagttttt gcagatgctt acagaaaaag gtgcaaaaac tctaagtgat gtaaacaata
     5641 tacttggaaa gattgattac acaaagctta gcacaaatca tataaccttg atagcaaaaa
     5701 attctatagg tataaaaaat ttatataaaa taatttcaga tgcacatgta aatcattttt
     5761 ttagagcacc aagaatcctt agaagtgtat taaatgaata taaagaagga ttaataattg
     5821 gttcggcttg tgaagctggg gtggtttttc aagctgtaaa gaaaaatgtg tctgatgagg
     5881 aaatgaaaaa aataatagat ttatacgatt atattgaagt aatgcctata gataataata
     5941 gatttatgat tgataaagga gaagtaaagg atgaagaaga gttaagggag ttgaatagga
     6001 aattaattga tacagccaaa aaatttgaca agattccagt agctactggg gatgttcatt
     6061 ttttagacaa acatgaagct gtcttaagaa aagttcttaa atattctcaa ggttttaaag
     6121 tagatgaaga agaaacttat ttacatttta gaacaacaga tgaaatgttg gaagagttta
     6181 gttatcttgg agaagaattg gcatatgaag ttgtagttga aaatagtaat ctgattgccg
     6241 atatggttga agatataaaa cctataccag atgatactta tcctccaatt atagagggct
     6301 cagatgtgga gttaagagaa atgtgttaca aaaaagccaa acgaatctat ggagacccaa
     6361 taccagaaat agttcaaaaa agactggata gagaattaaa ttcaataata agcaatgggt
     6421 atgcagttat gtatattata gctcaaaaac tggttgcaaa atctctttca gatggatatt
     6481 tagttggttc aagaggttca gttggctcat cttttgcagc tactatgagt gatataacag
     6541 aagttaatcc tcttccagca cattatattt gtgaaaacga agactgtaaa tattcattct
     6601 tttatgaaat aggtgaatgg ggctcaggtg ttgatttacc agacaaggat tgtccaaagt
     6661 gtggaagaaa acttgctaaa aacggtcatg atattccatt tgaggttttc ttaggttttg
     6721 aaggtgataa ggaaccagat atagacttaa atttctcagg aaattatcag ccaacaattc
     6781 ataaatacac agaagaacta tttggagagg ggtatgtata tagggctggg actataggta
     6841 ctgtagcaga aaaaacagct tttggatacg caagaaaata tgtggaagaa aataatataa
     6901 gtgtaccaaa tgcagaagta cttaggcttt caaatggatg tactggagta aaaagaacct
     6961 caggacaaca tcctggtgga gttatggtta taccagacta taaagatgta tacgatttta
     7021 cacctataca gtatccagca aatgatactt cttgtggtgt tataactact cactttgatt
     7081 atcattcaat aagtggtaga atacttaaac ttgatatact tggacacgac ggtcctacca
     7141 ttataaggat gcttgaagat atgactggaa taaatataac agaaattcct ttagatgata
     7201 aagagactat gtctctattt acatctacag aagcattagg tgttacacca gaagaaataa
     7261 actgtcctat aggttgcctt gctgtaccag agtttggaac taagtttgta cgacaaatgt
     7321 tacttgacac aaaacctact acatttgcag aacttgtacg tatatctggt ctctctcatg
     7381 ggacagatgt ttgggtaaat aatgcacaag acttagttag agaagatata gttggattaa
     7441 aagatgttat atctacacga gatgacataa tgaactattt aatatttaaa ggattacctc
     7501 ccaaaatgag ttttacaata atggagagtg taagaaaagg taaaggttta aaaccagaac
     7561 acattgaaga aatgaaaaaa aataatgttc cagaatggta tataggttca tgtaaaaaga
     7621 ttaagtacat gttcccaaaa gcacatgctg ttgcttatgt aatgacatca tttagaattg
     7681 catattgtaa agttcactat ccagaagcat tttatgcgac ttattttacg acaaaagttg
     7741 aggattttga tgcagattta attgttaaag gattagatgc tataaaatca aaaataaatg
     7801 aaattgagtc actgggaaat gatgcaacca caaaagaaaa aggtatgttg accgttttag
     7861 aagttgctct tgaaatgtat gctagaggaa taaaattatt accagttgat atatataaat
     7921 cagatgcaac tgagtttata gttgtaggag aaaaaacatt acttccacca atggcagcaa
     7981 tacaaggtct tggaggaaat gcggctataa acatccaaaa tgaaagaaaa aatggtgaat
     8041 ttatatcaaa agaagatttg agaaagagaa caaaaatatc aaaaacggta atagaaactc
     8101 ttactaatca tggttcactt gagaatatga gtgagagaaa tcaattgtca ttgttttaag
     8161 ttagcccaaa aagttgcaaa taaaagaatt ttatgatatc atagtataga aaatagtata
     8221 ctttgcagaa actaagagtg agacagctgt tctcactctt ttattttaaa tatattatta
     8281 aaaagctggt ttatttttat taaatatatt ttatttaaga ttatataaac tggtcattta
     8341 caattgaata ggaggaactt taatgaaaaa aaatttagaa gctacaattg aggagattgt
     8401 aactaaaata acggatgaac atggatttga aatggttgat gttgaatatg taaaagaagc
     8461 aggagaatac tacttaagag tatacataga taaagaagaa ggaatatctt taaatgaatg
     8521 tgaattagtt agtagagaat taagtccaat actagatgaa aaagacccta taaaagaaaa
     8581 ttacttttta gaggtatctt ctccagggct tgatagagca ttaaaaaaag acagagattt
     8641 tgttagatat caaggtagag atgtagattt aaaattatat aaaccattaa atggatgtaa
     8701 gcaatttgaa ggtgaacttg taggattaac agaagataat aatataaaaa tcattgcaaa
     8761 tggtaaggaa atggaattta atagaaaaga tgttgctatt gtcagattag caataaagtt
     8821 ttagtggtat attaaatagg gaggatataa aaaatgaatc atgaatttat ggaagcatta
     8881 gatgaattag tagttgacag aggtatagat aaagaaatac ttatagatac tatagaacaa
     8941 gctcttttaa ctgcatacaa gaaaaacttt ggttcagcac aaaatgttag agttgaattt
     9001 gatagagaca agggagatat aaaagtattt tctcaaagag ttgttgttga tgaatcagat
     9061 ttatatgata ctttcttaga aatagagtta gcagatgcta gagagataaa ccctaattat
     9121 gaattaggtg atattataga acatgaagtt acacctaaaa actttggaag aattgcagct
     9181 caaacagcaa agcaagtagt tgttcaaaga ataagggaag ctgagagaga aatagtgtat
     9241 aatgaattta tagaaaaaga aaatgaaata gttacaggag aggttgctag agcaaataaa
     9301 aatgttgttt atgtaaatct tggaagaata gaaggtataa tgactcaaag tgaacaaata
     9361 ccatttgaga attataaagc aggtcaaaaa ataaaattct atatacttga agttaaaaag
     9421 actaacaaag gacctcaaat agttgtatca agaagtcatc cgggtctagt aaagagattg
     9481 tttgaagaag aagttcctga aatatatgaa ggaatagttc aaataaaatc tgtagcaaga
     9541 gaagcagggt caagaactaa aatggcagta agatctatag atgaaaaaat agacccaatt
     9601 ggagcttgtg ttggaccaaa aggacttaga gtaaaaaata tagtggatga acttggcgat
     9661 gagaaaatag atataataaa atatagtgaa gacccagcag agtttatatc agcagcactt
     9721 agcccatcaa aggttgttag agtagatata gatgaagatg aaaagtctgc cttagtaatc
     9781 gttccagatt atcagttatc gcttgctata ggtaaagaag gtcaaaatgc aagactagca
     9841 gcaaaattga ctaactggaa aatagatatt aagagcgaat ctcaagcaga acaagaaatg
     9901 ttaaatcttg aaaattccaa agaaatagaa gctgaggatg ttgaagctga atttatcgac
     9961 caagaagagt tagatacttc agaggataat tttagtgaag aagagtaagg agggatgatt
    10021 ttgaaaaaag taaaaaaagt gcctcaaaga aaatgcatag catgtcaaga tagagactct
    10081 aaaaaagagc ttataagaat agttaaaaat aaagaaggaa aaatattttt agacccaaca
    10141 ggaagagcca atggaagagg agcatatgta tgtaaaagct ctgaatgtct aaaaaaagct
    10201 ataaagagta aagcattaaa taaagctttt aaaatagatg ttcctgatga agtctatgat
    10261 aatctactta tggagttaga agaatatgaa aaataataaa gaaaaagtac tttcgttctt
    10321 agggctcgca actcgtgcag ggaaaatagt gtctggtgat gattcaactt tgcttgacct
    10381 taaaaaagga aaagtaaatt tgattttaat cgcagaagat gcatcaaata atactaaaaa
    10441 actttttaaa gataaatcaa cttttagaaa tattccatat ctattttttt caacaaaaga
    10501 ggaaatagga tttgcaatag gaaaatctcc aagagcagtt gtaggaataa aagatgaaaa
    10561 tttttctaaa aagataattg aactaataga aatttaaaat aaaatagggg gtgatcttgt
    10621 gtcaaaaaca agagtatacc aaatagcaga agaattaaat atatcaaatg aagagcttat
    10681 aaataagtta gctgaattag atataaatgt aactgacaaa gattcagtat tagaaggtga
    10741 agagttagaa ttagcacttg aaatgttagg tgaagattta tctcaagaaa atggaaatgt
    10801 tatagaaata gatggaaagt tgacagtaca agtattagca acgaaattag ataaatctcc
    10861 atcagaaata attatgaagc ttatgaaaat gggaactatg gcaacaataa atcaagagat
    10921 aagttttgag attgctgctt tggctgccaa agactatgga tttgaattaa cagtagctga
    10981 aagtgatgat acagaagctt tagaaataga agctttaatg gaaattgaag aagataaaga
    11041 agaggattta aaacctagac caccagttgt tacagttatg ggacatgttg accatggtaa
    11101 gacatcttta ctagatgcaa taagaaaaac tgatgtgatt tcaggtgaag ctggaggaat
    11161 aactcagcat ataggtgctt cagaagttaa aatcaatgga cacaaaatag tatttttaga
    11221 tactccagga catgaggctt tcacatcaat gagagcaaga ggagcacaag ttacagatat
    11281 agcaatatta gtagttgcag cagatgatgg tataatgcct caaacagttg aagctataaa
    11341 ccatgctaaa gcagcaggtg tacctttaat agttgctatt aataaaatag ataaaccagg
    11401 tgcaaaccca gataaggtta aacaggaatt agcagaccaa ggattattag ttgaagactg
    11461 gggtggagaa gtaatagcag ttccagtatc agctaagaaa aaagaaggaa tagatacact
    11521 tttagaaatg gtattattgg ttgctgaaat ggaagagtta agagctaatc caaataaaag
    11581 agcagtagga actgttatag aagctgagct tgataaagga agagggccag ttgcaacagt
    11641 acttgttcaa ggtggtactt taacagtagg tgacccaatc gttgcaggtg ttgcatgtgg
    11701 taaagttaga gcaatgataa atgcaaaagg taagagagta aaaacagcag gaccatctac
    11761 agcagtcgag atattaggtc tatcagaagt tccacaagga ggagaccaat ttgtcgaagt
    11821 tccaactgat aagattgcta gaagtgtagc agcaagaaga caacaaatag ttagagatga
    11881 aatgttaaaa tcaactcaaa gattatcact agatgctcta tttagtcaaa tgagtgaagg
    11941 ttctataaaa gaccttaata tagttataaa agcagatgta caaggttcag tacaggcagt
    12001 taaacaatct ttagaaaaat tatcaaatga agaagttcaa gttaaagtaa tacatggtgg
    12061 tgttggagct gtaacagaat cagacatatt acttgcagca gcttctaacg caataataat
    12121 aggattcaat gtaagaccag ttccaggtgc agaatctcta ggagcaaaag aaaatgttga
    12181 tataagaaca tatactataa tatacaaggc aatagaagat atacaagcgg ctatgactgg
    12241 tatgttagac cctgaatatg ttgatgaaga aacaggaaaa gctgaaataa gagaaatata
    12301 taaaatatct ggtgttggaa cagtagctgg atgttatgtg actaatggta agatatttag
    12361 aaattgtaaa gtaagattag ttagagatag cataataata catgaaggtg agcttgcagc
    12421 acttaagaga tttaaagatg atgtaaaaga agttaattct ggatacgaat gtggtatgag
    12481 ttttgtaaat tataacgaca taaaagaagg cgatatagta gaagcttata taactaaaga
    12541 agtagaaaga aaattataaa ttcgtatcaa agaaaatcag ttagtaaaga aggtcgatgg
    12601 tatggcatca tataatagaa caagaaggat agcagaagaa ataagaaaag tcgtaagtac
    12661 tatgcttata aatggagtaa aagaccctag aataacatct atggtgagtg taactgatgt
    12721 agaagttaca aatgacctaa gatatgctta tgtatatgta agtattctag gcggagatga
    12781 agaatccaca ctaacaggtc ttaaaagtgc tggaggatat ataagaagag aagtaggaaa
    12841 aaatataaaa cttagatata ttcctgagat agtatttaaa cttgatgatt caatagaaaa
    12901 aggaatgtat atggatagcc ttataaaaag agttaatgaa aaaaatgctc aacaaaatga
    12961 ggatgaaaat tatgatgaat aatatggata gtattataaa ctatatttat gagggtgact
    13021 attttatagt tacctctcat atcagtccag atggagacaa tattggttct acactttcaa
    13081 tgtattatac attgaagaat ttaggaaaaa atgtatatta tgtacttgat gatgaagctc
    13141 cattaaattt aaggtttttg gttgaaggtg taaaaatcta taagtctaat gaatttaata
    13201 tgaagaacta ttctctaatt gcacttgatt gtggggataa gatgagaata tgtgtgagtg
    13261 atgaaatcaa aaataatgca tcaaaattaa tctgtattga ccatcatgct agtaatgatt
    13321 catatggaga tttaaattat gtagatattg atgcatcttc tacatgtgaa ttagtttata
    13381 atttattagt tagatttcag caaataaaag atattaatat tataaatgaa gatatagcaa
    13441 catgtttata tacaggtctt gtaactgata ctggaaactt ttcgtattca aatgtacatg
    13501 caagtagctt tgaaatggca aaaaatttac ttgttttagg tgcacaaaaa aatactataa
    13561 tacaaaatat atatcaaagc aattcctctg attattataa attattagga gaagctctaa
    13621 agggtttgga gatatttgat tcaaaggtat ctagtatagt tttaactcaa gacatgatga
    13681 atagaaataa tattagcttt aatgatgttg atggaataac aacatataca agagatataa
    13741 aaggtataga agttggaatt ttatttaaag agaaaaaaca aaatgaaatt aaagtgagtt
    13801 ttaggtcaaa aaattatgtt gatgtaagtg aaatagccaa gttatttggc ggtggtggtc
    13861 atgtaagggc tgctggatgt actattagag atagtattga taatgcaaag aaaatggtac
    13921 tagaggcagt gttaaaatca atttaaggtg acagtaaaat gaataagatt ataagtatat
    13981 tgaaacccac aggtatgaca tctcatgatg ttgtaagtag agtgagaaag attttaaata
    14041 taaaaaaagt tggtcataca ggtactcttg acccagatgc ttcaggtgtt ttaccaatct
    14101 gtatagggaa agctacaaaa gtatgtgaag ttattttaaa caaagataaa tcgtatatat
    14161 gtgagcttac tttaggtata agtacagata cttatgatgc ttcaggtgaa attttaaaaa
    14221 aagttgatga ttttaaattt agtaatgaag acatagaaag agcttttgat acacaaagag
    14281 gagagataaa tcaacttcct ccaatttatt cagctttaaa ggtaaatgga aagagaatgt
    14341 gtgatttagt aagaagtggt agacaatctg aaattacttt aaaaacaaga agagtaaata
    14401 taaaagacat aaaaatacta agtataaaag gtaataaagt aatgttttat gttgagtgtt
    14461 ctaaagggac ttatgtgaga agtatttgcc atgatatagg agaatatcta ggttgtggag
    14521 ctcatatgtc gtttttgaat agaacatctt ctggtaaatt tgacttagat aattcaataa
    14581 cattagaaga attagaatta ttttatgaaa ataaaacttt agacaaatac ttatatgata
    14641 tagattatgt ccttgattcg tttaattatg ttgtattaaa ccctaatgct ataaaatatt
    14701 atagtaatgg tggctcaata gatgataaaa ggtttttaaa aaataatttc gataaagatg
    14761 atgaatttgt aagagtatac agtacagata atttcttagg attaggaaag ttatcaaaac
    14821 acaataatac aataagtgtt aaaagtgata agatgttcat ataaccaatg tatttaaaaa
    14881 aacaacaaaa gagatagtac tataataact tctatagtct atctctttta cttatgctat
    14941 aatagtttgt gaataagtta agagtaaatt atattttagc ggggaacatt tattatggtg
    15001 attataaaat ctataaaaga aatagcaaac ataaaagaaa gtgtcattac cataggaaat
    15061 tttgatggtg tacacaaagg acatcaagtt cttataggaa aaacagttga acatgcaaaa
    15121 aaagaaaata tacagagtat tgtatttaca tttgcaaatc atcctgtaaa ttactttaga
    15181 ccaaactcaa caaaaaatat tataagcaat gatgataaaa taaaattatt agaagatttg
    15241 ggaatagata tagttgtaat cataccattt gatgagtata tgactaaaat acctgcaaag
    15301 gatttcgtag atgagatact agtaaaaaaa ttaaaagcta aaaagatagt aattggacat
    15361 gactttacat ttgcaagggc aaaagaaggc aatgttaatc tattaaaaag cttagaacat
    15421 gaatttggtt ttgaggtaga agtaataaaa cccatcaaaa tcaatgatgt aagagtgagt
    15481 agtacttata ttagaagttt agtatcccag ggtgatatgg ctaatgtcaa ggaatattta
    15541 ggtagaaatt acaagttaga aggttgtgtt atacattcta aacaccttgg aagaactata
    15601 ggattcccaa cggcaaatat tgaccttaag aataatatgc tcgttccaaa gagaggtatt
    15661 tatgcttcta ttgtacatat tggagatgag acttattttg gtgctacaaa tgtaggctat
    15721 aacccaacag ttaatggaaa aactttgtct attgaaacaa atattttaga gtttgataga
    15781 gatatttatg gggaaaatat aattgttgaa tttttggaaa gaataagaga tgaaagaaag
    15841 tttaattcaa tagatgactt aaaaaatcaa ttgtataatg atacaaatta tgtgtatgaa
    15901 aactatgttt gcaaaaacaa gtaatgtatg ataaaatgat taaggaaaat aaccattact
    15961 cggcatttga atactccaat caatgcttag taataggcgt ttctaaaaaa atacggaggt
    16021 aatctaaatg ataaacaaat cagaaataat aaaagaatat ggaagagctg aaggggatac
    16081 tggttctcca gaagtacaaa tagctttatt aacagctaga ataaacgagt taaatggaca
    16141 tttaaaaact cataagaaag accaccattc aagaagagga ctattaaaaa tggttggtag
    16201 aagaagaaac ttattagctt acttaaagga aaaagatctt gaaggatata gagcattaat
    16261 agctaaatta ggattaagaa agtaatttca ttctttataa aaaagcaggt atagtgtata
    16321 cctgcttttt tatagttaat tcattataaa gtaaaatagt taataataaa agtagtttat
    16381 atgttataaa aaattataaa atgaataata ttgggtaaga atattaaaat agtatacaaa
    16441 tacataatca atttaaaagg aaagacagga taaatataga aattttataa caggataatt
    16501 agttggaata aggaggatat tattttgaaa ataaagttag tatgcgatag tttgtgcgat
    16561 attccagatg aaatatcgga aaaagattat atagagatag ttccacttac agttattttt
    16621 aatgataggg aatatataga aggagtagat ataaagaaag aagagtttta taaaaaagta
    16681 aaagaaatca agcaaatacc taaaacttca caagctacat atatggagtt taaagaagtg
    16741 tttgataagt tcataacaga aggttatcat ataatctgta tgacaggggc tgcaaacgca
    16801 tcaggaactt ttcaaagtgc tatgatagct aaaaatgatg taaatgaaaa tgaaaaaata
    16861 catatatttg atactagaaa tttatctcta ggtagtggac aatatgttat aaaggcatgt
    16921 gaacttcttg aagaaggact gggatttgaa gaaattatag atgaattaga aaatactcgc
    16981 agtagtgtaa gattgctttt tgcaccatat acacttgatt ttttaaagca gagtggaaga
    17041 gttccagttg caactgcttt gataggaaat atgttaaata taaagcctat atttttcttt
    17101 gataatgggg aagctaagtt ggtaaataag gttagaggaa taaagaatat agcaagtaaa
    17161 ttggtcgatg taattttaga aatgaatgaa gggtctttag aaggaaaaat cgtcacaata
    17221 ggttgtggag ataatttaca cgattgtgaa atattaaaag atgaagtaaa taaaagaata
    17281 aaagcaagaa gagtattatt tactcgagga ggagtttcta tttgctctca tacagggcca
    17341 gatatattgg ctataagttg ttctaattag tagtaatata aggtttttgc atatatatgt
    17401 gaagataatt ttatattgtt aatttatagt atttttgata atatagtaaa tagagtgata
    17461 tacgtgtttt ttaataagag gaggacaagc attaatgttt gaacataaaa tatttaaaat
    17521 ggattttgca ggaagagaac tttctgtaga aataggaaag atttgtgaaa tggctagtgg
    17581 tagttgtata gttcgataca gtgatagtat ggtaatggta aatacaacta aatcagccaa
    17641 acctagagac ggaatagact tttttccttt aagtgtggat tatgaagaaa aattatactc
    17701 tgtaggtaag atacctggag gttttttaaa gagagaaggc aagccttcag aaaaagctat
    17761 acttacatca agattgatag atagaccaat aagaccttta ttcccaaaag gatttagaaa
    17821 tgatgttcag gtagttgcaa cagttctatc agtagaccaa gattgtacac cagatatagt
    17881 agcaatgata ggttcttcaa tagccctttc tatatcagac ataccattta atggaccaac
    17941 aggttcagta tgtgttggcc ttgtagatgg tgcatttgtt gtaaatccaa acgcagagca
    18001 aagagaaaaa agttcaatgc atctagttgt ttcaggtaca aaggaagcaa taatgatggt
    18061 tgaagccgga gctgatgaag ttccagatga ggtaatgctt gatgctattt tatttgctca
    18121 ccaagaaata aaaaaaatag ttgagtttat agaaggtata gttgcagaag ttggaaaaga
    18181 aaaaatgcct gttgaattat accatgcagg agaagaaata actcaattgg ttcgcgaatt
    18241 tgcaactgat aagatgaaaa aagcagttca gacttttgaa aagttagaga gaatggaaaa
    18301 tatggataga gttaaagaag aaactttagc tcactttgaa gaaactttag aagattttga
    18361 agattttgtt ggagatatag aagaagtatt acaagatata ataaaagaag aagtaagaaa
    18421 acttatagta catgaaaatg taagaccaga taatagaaaa cttgaagaaa taagacctat
    18481 atggtgtgaa actggcatga tacctagagc tcatggttca gccatattta ctagaggaca
    18541 aactcaagta cttaatgttg ctactttagg tgctttagga gatgttcaaa aactagatgg
    18601 gttagatgaa gaagaaaaca agagatatat gcatcattat aatttccctg catatagtgt
    18661 tggagaagct agaccatcaa gaggaccagg aagaagagaa ataggacatg gtgctttagc
    18721 agaaagagct ttattaccag taataccatc tcaagaagaa tttccttatg ctattagatt
    18781 agtttctgaa gtattaagtt caaatggttc tacatctcaa gcaagtgtat gtggctcaac
    18841 attatcttta cttgatgcag gtgtacctat aaaagatatg gttgcaggta tagcaatggg
    18901 acttataaag catgatggta aggttgcagt gctttcagat atacaaggta tggaagacca
    18961 tttaggcgat atggacttta aagtagctgg aacagagtat ggtataacag caatacaaat
    19021 ggatataaaa attgatggta tagataaaga aattctacaa cgtgctttaa aacaagctaa
    19081 agagggtaga atccatatac taggagagat gagaaaaact atatctcagc caaaaccaga
    19141 actttcacca tatgcaccta agattgttaa aatgcaaata aatcctgaca agataaaaga
    19201 tgtaatagga cctggtggaa agataataac taagataata gacgaaactg gagttaagat
    19261 agatattgaa caaactgggg aagtatttat atcaggtatt gagattgata tgattaagaa
    19321 agctcaagaa cttataaata atatagtagt tgaaccagaa gtaggaaaaa cttataaagg
    19381 taaggtaagt agaataatga actttggagc atttgttgag atacttccag gaaaagaagg
    19441 attacttcat atatctcata tagctcatga aagagttgcc aaagttgaag atgtattgaa
    19501 tataggggat gaagttgaag ttaaagttac agagattgat gaaaaaggaa gagttaattt
    19561 atcaagaaaa gttcttttac caaaaccaga acataaaaat aaataactta ttatataatt
    19621 aaaagcaaag tcttaggatt ttgctttttt aaatacaaaa aagtagataa ttttaaaatg
    19681 atagttatgg atggcttata gttctttttc tgtcaggctt tactcttata aagaagctat
    19741 ttaaaagctg attaatagag agctttattg atatatatag aatcacttaa gttatattta
    19801 gtgcataaac agagcttatt tttaatagta ttaaataaga aacaatttgt acataattag
    19861 ggggagtttt aatgataatg atggtactta ataagagtaa gctcaaaaaa atcataatac
    19921 taattttagt agtgttatta gtattatcag gaggggtaat ggtattaaag ttatttaaca
    19981 acaagccagt atttaattta tttaatggtg tagatttacc atatgaaaga ggtacaaagg
    20041 ataaaagtgg atatgttgca ttaacctgta atatagacct tggatgggaa acagagtacg
    20101 tagaatccat tttagaaaca cttaaaaaag aaaatgtaaa gattacattt aatgtaactg
    20161 gaaaatgggc agaaaagaat aaggatgaat tattaaagat aaaaaaacaa ggacatgaaa
    20221 taggtaatca tggatacaaa catttagatt attctacatt atcatatgaa gacaattatg
    20281 agcaaataga gacctctaaa aagataattg aagaaattat aggtgaaaaa actaaatttt
    20341 ttcaagcacc agcaggttcc tttggtccag aaacagtaaa ggctgctaaa gcattagggt
    20401 atacttctat caaatgggat gccgatacta tagattggaa atacaaagac caaccagagg
    20461 taatcattga tagaatgaag aaaaaagaca ttaaagatag tagtataatc ttaatgcatc
    20521 caaccaacgc aacgacaaaa tgtatagatg atattatagc tattgtaaga gaaaaaggac
    20581 taaaaccagg aaaacttagt gatgtattta agtagacaaa gtataaatat ttggttaaga
    20641 ttgtataagt tgtagttgaa ttaattaaaa gactgactta taatgtaata catataaaaa
    20701 ataaatataa ttaaaacata gtgttttcaa taatccaata tgaaacaaag aataaattgt
    20761 taggagaatg taatgtataa gacaaaaata ctggaaaatg ggcttacaat aattggtgaa
    20821 gaaataccat atctaaagtc aataacatta ggtatttgga taaatgcagg ctctaggata
    20881 gaagaagcac aggtgagtgg aacttctcat tttatagagc atatgatgtt taaaggaacc
    20941 aaaaacagaa catcaaaaga gattgctagt tcaatagata atttgggtgg tcaaataaat
    21001 gcatttacaa gtaaagaatg tacatgttat tatgtaaaac taatagatga gcatatagat
    21061 acaggaattg atgtattgag tgatatgata ctaaattcta aatttgataa aaatgatata
    21121 gataaggaaa gacttataat attggaagaa cttaagatgt atgaagactc tccagatgac
    21181 ttatcttatg atttattagt ggaaaatata tatgctaatg atggattagg aatgaatata
    21241 attgggacaa aagaatctct atacaatata actagagaat ccatgttaga atacttaaat
    21301 aagtattata ttccaaataa cgctgttatt tctatagctg gaaactttaa ctttgatgat
    21361 atggtggaga aaatcaaatc aaaatttgga cactgggaaa agaaaaattt gagtatagac
    21421 ataagtgagg ctaagtttaa tccttgcttt atatcaaaaa ataaagatac tgaacaggtg
    21481 aacttagcta tgtgcctaaa aggtattcct tttgaaaatg atgaagaagt ctactcaatg
    21541 gcagtggtaa ataatatttt cggtggaagt ataagctcaa ggctttttca gaaaataagg
    21601 gaagaaaaag gtttagtata ttcaatatac tcatcacaga cattatatag gaaatgtggt
    21661 gaattaggta tatttgcaag catgagtaca gaaaatttac aagatgtata taatttaata
    21721 aagaaagaaa ttgaaaatat aagagaaaac tatcttacag aaaaagaaat atctgaaagc
    21781 aaggaacagt taaaaggaaa ttatatattg gatttagaaa gtactagcag tagaatgatg
    21841 tctactggta aatctatgtt acttagtaaa aaagtaaaaa ctacagatga aatacttgaa
    21901 tgtataaata atgttaatat taattcaata aagaaggttg ttgataaggt attcaatatc
    21961 gaaaatattg gtacatgtat agttggaaga gatgtagaaa agattctaca cttggattaa
    22021 tattgaaaaa agtaaatctc tggaggtata aaatatgaat ttatctgaaa tagcaggtaa
    22081 ggaaatagtt aacttggtca caggagaaag attgggggtc attggagaat gtgaccttat
    22141 actagatgaa actacaggcg gtatattagc gcttctaatt ccaaaagaaa aaggattttt
    22201 tgccagaaga aaagataagt catttttaga agtaccatgg agaaatgtaa aaaaaatagg
    22261 taatgacatg ataattatag aatatgaggg atatatttag gaggaaaaaa tgagtatttt
    22321 agtgcaaaag tttggtggga catcagtaga atcatatgaa aaaatgaatg aagtatgtaa
    22381 gatagtaaaa gcatacaaaa aaaatgatga agaattacaa ttagtattag tagtttcagc
    22441 aatgggaaga aaaggagctc catatgctac agatacactt ataaatttgt gtagtgcagt
    22501 aaatgatgag ccaagtaaaa gagaacttga tttaataatg tcttgtggag aaataatatc
    22561 aggaacaatt cttgctaatt tattgaatgc acaggggata gattctgttt tctttactgg
    22621 tcagcaagca ggtataatta caagtgatga atattctaat gcaaagataa agtatataaa
    22681 tcctaaaaag gtaaaaagag ctttagatga tggtaaggtt gtagttatag ctgggttcca
    22741 aggtgtaaca gatgatggag aaataactac tcttggaaga ggcggaagcg atacatcagc
    22801 agttgctatt ggtaaagctt tagaatgtga aacagttgaa atttacactg atgtggatgg
    22861 tataatgact gcagacccta gagtagaacc aaacgctaaa gtattgagtt ttatagacta
    22921 tgaggaagta ttccaaatgg ctgacaaagg tgcaaaagta atacatccaa gagcagtaca
    22981 gttggctaaa agtggaaata taactttagc cataaagaat acaatgaatc caacttttga
    23041 aggtacaaaa ataggttctt tatgtagaca cttagaagac aatatagaat atgaacaaga
    23101 aagagatttt aaggttgctg ttgcaaataa agatagcgta gcacaagtta agattaagtc
    23161 agcagaagaa gtgtttacag aagtattaaa tgagatagaa aagaaactta taactataga
    23221 tatgataaat ttctttatat cagaaaaagc atttgttgta gaagatgctg atataaaatc
    23281 attaaaggaa atactagata aatttgaatt agattatgaa gtaaatagag actgtgctaa
    23341 ggtaacattg atatgttcaa gaattgctga tgaaatgtct ggtatcatgt caaaggtagt
    23401 tagaggatta tcaaaagcag gggtatctct tttacaaaca tcagactcaa atatgactat
    23461 atcttgttta gttagtgaag aagatatgca tacagcagta catgctatac atcaacaatt
    23521 ttatttaaaa taaactgtat aatacaatta ttttttaatc aaatttaaga atttcatata
    23581 tatcttaata tatttgattt atttggtaaa actaaattaa atataatttt gataggagtt
    23641 tttttatgaa agattataga gaagataatg aaagatttga taaagataat tgtagtgaag
    23701 aatctagttg cgaagagaga gaatgtataa agcaatttgg taccaatgaa atgccacctc
    23761 aacctcctaa agatattcaa tgtataacta taattggaga aattgaggga cattttatag
    23821 gaaatcctca aaaaaaagct acaaagtacg agcatataat tccaatgcta tactcaattg
    23881 aggaaagcaa tgatgttaaa ggagtgttag ttgttttaaa cactgttgga ggagatattg
    23941 aagcaggttt agctattgca gaacttttaa acagtacatc taaaaaagta gttactcttg
    24001 ttctaggagg aagtcatagt ataggtgttc ctcttgctac agcaggagat tactcattta
    24061 tagcaccaac agcaaccatg attatacatc cagtgcgaac tactggtctt gtgattggta
    24121 taaatgagac ttttgaatat tttaaaaaga tgcaagatag aataattcaa tttataataa
    24181 gaacatcaaa tatcaaaaaa gatgtacttg aaaaacttat gcatgaaaaa gatgaacttg
    24241 taagtgatgt tggtagtgta ttaataggaa aagaagctgt ggattatggt cttatagatg
    24301 aagttggagg actaaaagaa gcacttaaaa aattaagaga gcttataaaa gaatctgagg
    24361 aagaaaaaaa taatgattaa aatttgttta aataataaaa tttatgatat aatgatgtaa
    24421 taaagtagga gtgttagtgt taaggcatta gcactttttg ctgtgaaggg gtaaaacttg
    24481 ctaaaaataa ggagtgatat taatggccac gaaaaagaag aaaaaaaaga aggtaaatac
    24541 cttaagtttt aatgcagaat atcataattt aataacaata tttataggtg tatttttatt
    24601 atatagttta aattctaatt ctatgggatg gatacctgtg ttaatgcaaa atctttttaa
    24661 gggactattt ggagggcttt caatagcaat accatttata gtaattataa caggactttt
    24721 aggatttttt gatggaaatg aatatatata taggctaaga aagactaaac tatactacat
    24781 aattatttta tttatatttg tattttatgg attattaaat gcaggaactc ttcctgtaga
    24841 tagcccacta aaaggtaaca tgtttgatga tgttatgaaa ttgggtgtaa gtggacaagg
    24901 tagtggtttg atagcaacaa ctattgcgta ttatatgagt aaaatatttg gtatagcagg
    24961 tggatggtta ataagtatct tcgcacttat actttctgta atgtttatat ttaatatttc
    25021 tataaaagat ttggtgtcaa atgcaaaatc aaaatctaaa gcatctaaag acagcaattt
    25081 aacgtttaaa gataaaattg caaatatgaa aaaatctgca atagatatga tgactgatga
    25141 agtagatgat actactataa ataaaaaacc aggtttcttt aaagggttaa tgtcaaaagg
    25201 tcttaataat gatgatgaag aagatgaata ttttgaagaa gaaaatagtg atgatgtaga
    25261 tgataaaact ataaaaatag ttggatttaa taaggcagaa gatgagtatt tagaaatatt
    25321 agaaggaact caaagtatgc cagagttaga tgttttaaaa gaattacaaa aagctaccaa
    25381 tgaaaatcca gttgtagata ctaaaccaga aaaaaaagtt gatatagcta aacctaattt
    25441 aaatatagaa aaaactcaac ctatgagtat tgtagcagag ccagttaatg aggattattc
    25501 aaactataaa aagcctagca ttgagctttt aaataaagta aataagaagt ctgatgaaaa
    25561 tggtaaaaag aaagtgttga aaaatgcttc tcttttagaa aagacactat cagattttgg
    25621 agtggaagca aaaattaatc aagttacagt tggacctact ataacaagat atgaaataca
    25681 accaagtcca ggagttaagg taagtaaaat tgtaaattta actgatgata tagctttaag
    25741 tttagcagct aaaagtataa gaattgaagc cccaattcct ggtaaaagtg caattggaat
    25801 agaagttcca aatgaagaag ctcaaatggt tggtgtgaga gaagttctag aaagtgaaga
    25861 atttaataat tttaactctc ctcttgcaat gggacttgga aaagacgtag caggaaaaat
    25921 tataattgga gatataggta agatgccaca cttactaata gctggttcta caggttcagg
    25981 aaagagtgtt tgtgtaaata cattaataag tagtatttta tataaggcaa atcctgatga
    26041 agtaaaatta ctattaatag acccaaaagt agttgaactt gcaaattata atggaatacc
    26101 acatctattg ataccagtag ttactgaccc taaaaaagct gcaaatgctt taaattgggc
    26161 agttacagag atgaatagaa ggtacaaatt atttgcagat gctcaagtaa aagatgtaac
    26221 aagttataat gagaaagcgg aagaaaaact tcctaaaata gtaataataa tagatgagtt
    26281 agcagattta atgatggcaa gtgcaaatga tgtagaagat tatatttgta gattagctca
    26341 aatggcaaga gctgctggaa tgcacttgat tgtagcaact caaagaccat ctgttgacgt
    26401 tataacaggg gtaataaagg ctaatatacc atctagaata gcatttgcag tatcatctca
    26461 aactgattca agaacaatcc ttgatatggg tggtgcagag aagcttctag gtaaaggtga
    26521 tatgttattc tatccacttg gtgcagcaaa accagttaga cttcaagggg cattcatatc
    26581 agagagtgag tcggaaaaag taatcgattt tgtaaaaagt caggttaaag atggaataaa
    26641 atatgaagaa gatataattg aaactatatc taaggttaat acttcaaagg gtagtgatga
    26701 ggatgagttt ttgagtgaag cgatagaatt tgtcgtggaa agtggtcaag catctgcctc
    26761 tatgctacag agaagattta aaataggatt caatagagca gctagactta tagattctat
    26821 ggaagaaaga ggaataattg gagcaagtga gggaagtaaa ccgagaaagg tattaataag
    26881 caaacaggat ttgcagaatt tagaaggtga atagaattgt cagttataaa aatagaaaaa
    26941 gcacttaagc tagataaagt aatatttgta gatgtgagaa ctgaaggaga atatgaagaa
    27001 gaccatatat tgaatgcaat caatatgcct ctttttaaaa ataatgaaca taatgaagtt
    27061 ggaacaattt ataagatgca gggaaaacat gaagcaatac aaaaagggtt tgactatgta
    27121 tcgtataaat taaaggatat atatttacaa gcagcagaac ttgcattaaa ttatgataat
    27181 attgtcatat attgtgcaag aggcggaatg agaagtggca gtatagtaaa tttattatca
    27241 tcattaggtg taaatgtata tcaacttgaa ggtggatata aggcttatag aaattttgtt
    27301 ttagagtatt taaggcatgt aatggataca aaaaactttg tatctgttca tggattgact
    27361 ggtacaggaa agactgattt gttacattta ttagatgaaa aaggaataga tgtactggat
    27421 ttagaaggca tagctaaaaa cagtggttct acctttggtt ttataacttt tgataaaaag
    27481 ccaccatctc aaaagaactt tgaaacaaaa ctatttgaaa gtatattttt ttcgaaagag
    27541 aattatatat ttgttgagag tgaaagcaaa cgagttggaa gtgttacagt tccaaatgaa
    27601 atttttgagg caatgacacg agaaggacat catatattat tagaatgtga tattgaaaat
    27661 agagttgata gactttgtag agattatatt tatgataaag atgaaggtaa tatattgatt
    27721 ttaaaagaat gtataaacaa gtttagaaaa agattgggta ataaagtagt agatgattat
    27781 attgatttat tagaatcagg tgaatataaa gaacttgtaa aaagatatct tttagaatac
    27841 tacgacccac tttatatgca ttcagtagag aaatataagt atagtaagat tataaatttt
    27901 aatgatacac aaaaagcttt agaagatgtt atagacttat atgagagtat attggaagga
    27961 gaaagtgaat gttaaaaata gcattggaat cattaggatg ctcaaaaaat ttagtagatg
    28021 cagaaataat gatgggaatt ttaaataaca aaggctataa attaattgga gattttgaag
    28081 aagctgatgt aattatagta aatacatgtg ggtttataga gtcagctaaa caagaatcta
    28141 tagatacgat aataaacttt gcagagttaa aaaaaactgg caatttaaag ttgttgatag
    28201 taacagggtg tctagcccaa agatattctg aagaattaaa aactgaaatt cctgaaatag
    28261 atgctatagt aggaacagga agctatcaaa atatagataa aatacttaaa gaattaagtg
    28321 aaatgcatca aattgtaagc cttaatgata tagagtttgt atttaatgaa gatttaccaa
    28381 gatatatatc tacacctagc tatatggcct atttaaaaat tggagaagga tgttccaata
    28441 attgtactta ttgtataata ccaaaactaa gaggtaaata tagaagtaga aaatttgaag
    28501 atataattaa agaagctaag aaattagctg aatctggagt aaaagaactt gtggttatag
    28561 cacaggatac gacaaagtat ggttttgatt tatatggaaa agaaagatta tcagaacttt
    28621 tagaagaact tgcaaaaata gatggattta agtggataag ggttatgtat tcatatccag
    28681 aatctataac agaggaattg atacaagtta taaagaaata tgataatata tgtagttact
    28741 ttgatatgcc aatacagcat gcaagtaaca atattttaaa attaatgaat agaaaaacta
    28801 ctaaagaaga tatattaaat aaaatcaatc tgataagaag taatatacca gatgctatac
    28861 ttagaactac aataatagta gggtttcctg gtgaaacaga agatgacttc aaacaacttg
    28921 tggactttgt tgaagaggtt aaatttgata gacttggtgc atttgcatat tcaagagaag
    28981 aagatacacc agcagataga ttgccaaacc atatagatga agaagtaaaa atacaaagaa
    29041 gagatacttt aatgatgatt caacaaaaaa tatctgaaga acttaatgac aaaaagatag
    29101 gaaaaactta tgaggtgtta atagaagaac agatagaaga taatgtatat acaggaagaa
    29161 cacaaggtga tgcagaagaa attgacagta tagtgtatgt aaaaagtgta gacaatcttg
    29221 aagtaggaga atttgtaagt gttcaaataa atgatgcaat ggaatacgat ttaatgggag
    29281 atgttttata tgaacttgcc taacaagtta actttgttta gaatattttt aataccagta
    29341 tttgttttaa ttatgttgct caatgtacca aataaatttt taatagcttg tattattttt
    29401 ataatagctt caattactga tgctttagat ggaaaaatag ctagaaaata caatctagtc
    29461 actgactttg gaaaatttat ggacccatta gcagataagt tattggttat ttcagcactt
    29521 acatgtatga tagaagacca tcttgtaagt agttggatgg taattataat agtggctaga
    29581 gagcttacag ttagcatatt aagagctata gctgctgctg atggaaaagt aattgctgct
    29641 ggaaacagtg gaaaacttaa aacgataact caaatggtat ctatagtgtt tttattgtta
    29701 ggtgctcagt ttgaaaatgt attgatatta aatattggtg aaattctcat tcttatagct
    29761 acactactta cattatattc tggatgggaa tatctatata aaaataaaga gctttttatg
    29821 tcttctaagt aaaaaattat gctttaataa ataaaaacta atgattaata aataaaaaat
    29881 aacaacttaa aattttaaaa atgtaaaaaa gggtatacta ttatagtata cctcttttca
    29941 ttttaagaat attttctttt gtgtatataa ttattaataa tggtttataa ataaaatgta
    30001 atgaataaaa atataatcat tagtagcgtt tttaaattga ctaggtttga tttatgttat
    30061 ataattaata tataacaaga gaacaaatgt ttgtagataa atgaagggat ggtatagaga
    30121 tgagtgtaga tcaagaaaaa ttaaaagcgt tgaatgaagc tttaggtaaa attgaaaaag
    30181 attttggtaa aggttcagta atgaaattgg gagaagcaac atctatgtct atagatgtta
    30241 tatcaacagg agcgattggt ttagacatag ctattggtat aggaggtcta cctagaggga
    30301 gaatagttga agtatatggt ccagaatctt ctggtaagac tactgttgcg cttagttgtg
    30361 tagcatcagc tcaaaaagat ggaggaatag ctgcatttat agatgcagaa catgcacttg
    30421 acccagtata tgcaaaagct ttgggtgtgg atgttgataa cctaataata tctcaaccag
    30481 atacaggtga acaggcttta gagatagcag aggcattgat aagaagtgga gcgatagata
    30541 taatagtaat agactcagta gcagcattag ttccaaaggc tgaaatagat ggagatatgg
    30601 gtgattctca tgtaggttta caagctagac ttatgtcaca agcacttaga aagttaactg
    30661 gttcaattaa aaaatcaaat tgtgttgcta tatttataaa ccagttaaga gagaaagtag
    30721 gaataatgtt tggaaaccca gaaactacta ctggaggacg tgcactaaaa ttctattcat
    30781 cagttagatt ggatgttaga aaaatagata caataaaaca aggtgataaa gttataggta
    30841 gtagaactag agttaaagtt gttaaaaaca aagtagcacc accatttaag caagctgaat
    30901 ttgatataat gtatggagaa gggatttcaa aaattggaga ccttttagat atagctgctg
    30961 atgtagatat agttaaaaaa tcaggttcat ggtatagtta caatgatact aaacttggac
    31021 aaggaagaga aaatgttaaa aaattcttgg aagataattt agatttaact actgaaatag
    31081 atgagaaagt tagagcattt tacaatttaa atgaagaaca tgaagaatca ggtacttcag
    31141 tatcaaaaga aattgtagaa gaataagtaa aaatacaaaa aaataactag taattaaaga
    31201 catgccacgc tccctattag cttgacacaa tgttgaaaaa attataaaat taagtgagga
    31261 taaaatatct aaatttaatt ttaattatta ggaatatttt ttaatcatag tgatgacatt
    31321 ttgggattat taacgaagga ggtggatgtc atagatagtg tagtaatgat tgtaataggt
    31381 gcagctgttg gtattatagc tggatatttt gttagaaaaa atatatctga agcaaaaatt
    31441 ggacaagctg aaaacttggc caaagaaata atagataaag cacaccacga ctcagaaaca
    31501 gtacaaaaag aaaaattact agaagctaaa gaagagatcc ataagtggag aacagaagct
    31561 gaaagagaaa acagagaaag aagaacagaa gtacaaaagt atgaaaaaag agtagtacaa
    31621 aaagaagaag tcttagatag aaaattacaa aatttagaat ctaaagaagt aaacctaagt
    31681 gaaaaattaa aggtagtgga aaagaaagaa gaagaagttg aagtaataaa aactcaacag
    31741 ttagaaaaat tagaaagtat atcaggaatt acttctgata aggccaaaga aatcatctta
    31801 accaatgcgg agagagatgt aagacgtgaa atgtctataa tgataaaaga gatagaaagt
    31861 caagctaaag aagaagctga taagaaatca agagaaatta taggatatgc aattcaaaaa
    31921 tgtgcagcag atcacgtagc cgaaactact gtaacagtag taagcttgcc taatgatgaa
    31981 atgaaaggta gaataatagg tagggaaggt agaaatataa ggactttaga aaccttaaca
    32041 ggtatagacc ttataataga tgacactcca gaagccgtaa ttttatcagg atttgatcca
    32101 ataagaagag aaattgcaag aattgctcta gaaaaattaa tagcagatgg aagaatacat
    32161 ccagctagaa ttgaagaaat ggttgaaaag gcgagaaaag aagttgacaa tattataaaa
    32221 gaatatggtg aacaagctgc atttgaaact agtgtacatg ggattcatcc ggaattaata
    32281 agattattag gtagacttaa ctatagaact agttatggac aaaatgtact taaacattct
    32341 atagaagttg ctcatatagc tggtataatg gctgctgaaa taggagcaga tattagattg
    32401 gctaagaggg caggtttatt acatgatata ggtaaggctg tagaccatga aatggaaggt
    32461 acacatgttg aaataggtat ggatttactt agacgttata aagagtctaa agaagttata
    32521 catgccatga gtactcatca tggagattac gagcctcaaa cagtagaggc tgtattagtt
    32581 acagcggcag atgcaatatc agctgctaga cctggtgcga gaagagaaac tttagaagca
    32641 tatataaaga gacttgaaaa attagaagaa atagctaatt catatgaagg tgtagaaaaa
    32701 tcatttgcta tacaagctgg tagagaaata agaataatgg taaaaccaga agctataaat
    32761 gatgaagaaa tacatctatt ggctagagat atgactaaga aaatagagga tgaacttgaa
    32821 tatcctggac aaataaaagt aagcataata agagaaacaa gagctataga gtatgcgaag
    32881 taaaagaaat aatatctaaa taataagaaa atatttttag aaatttaaat ttgccacaag
    32941 atatgataat atatcttgtg gcttttaaat tgtttaagtt taattttata taatgcaaaa
    33001 ataatctaag tgctaaaagt gctact
//
LOCUS       DAEMHW010000025        32391 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEMHW010000025 DAEMHW010000000
VERSION     DAEMHW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.025"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(254..3715)
                     /gene="cbpA"
                     /locus_tag="KPK67_000972"
     CDS             complement(254..3715)
                     /gene="cbpA"
                     /locus_tag="KPK67_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HBG2457973.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAEGDTNAGNAGGDANAGNAGGDANAGNAEEDSNAGNAEGDANAGNAEED
                     MNALVVEEATAEDPTSVGDFKISGGEINVDFKFASGVLIILSGKQMTIQNNNPNTPTT
                     HRIVIPKDIKAELIFDGVNIKTASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVY
                     YPGMRAGKGTTLIIDDSVVNRDIEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDS
                     NNRGSLKVVGNYYSAGIGGGNTESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNG
                     SGGDITINGGTITAKGAYHGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGL
                     SYSYGGAHGSAFGDGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVT
                     GGSLKATTFKSLGRAVAYGDLEKKTKVFMNKISLKYLGQDKVSTTLVDDFKMLIDGKI
                     YPYGAPSYTDDQGVLYLWFSDQFKGSEVSVNLVVIDKITGGNIKPDDFYVKDIGSGSQ
                     FLKQYEEFTIDDTPPYLIIKRYDGLPFEEDVEKSFLDWILKDGGIPIITPKGEKLTDK
                     KFMTISSQRLNEETMEPDTNLGSDDLTKQVDAGKYQLSITSTQFSNVYPFNEAYWGHR
                     AYYKAEIIPADTETSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQ
                     FYINGKEYKDPVELKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDI
                     NYIEGSEDEKKLKLDVTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTD
                     NTGEWKPSTEGGNGDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNV
                     DTGSGGYDTGNPNLNKPDPDNGGNSKPDPDNGGNGKPDSGNGGNSKPDSGNGGNSKPD
                     SGNGGNSKPDSGNGGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSK
                     PDPDNGGNSKPDINIDTDGDGKPDINIDTDGDGKPDVNIDIDGDGKLDVNIDIDGDGK
                     PDINIDTDGDGKPDINIDTDESLDSNVGQNVQTGDQSNIMLDLALMFISLFFLIKNLT
                     NKYLRRK"
     gene            3985..4557
                     /locus_tag="KPK67_000973"
     CDS             3985..4557
                     /locus_tag="KPK67_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HBG2457974.1"
                     /translation="MKKLVKILINIICIIALTFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNSNYEFKDYLNYIT"
     gene            complement(5571..6233)
                     /locus_tag="KPK67_000974"
     CDS             complement(5571..6233)
                     /locus_tag="KPK67_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2457975.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(6250..8805)
                     /locus_tag="KPK67_000975"
     CDS             complement(6250..8805)
                     /locus_tag="KPK67_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2457976.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNLVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNRLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPNDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLFRLMGAKKRNVRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGIISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDETLNDFVEEGRLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLIAVVVSNRDFESMNIIEGIKENE"
     gene            complement(9039..9233)
                     /locus_tag="KPK67_000976"
     CDS             complement(9039..9233)
                     /locus_tag="KPK67_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG2457977.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTKEN"
     gene            complement(9245..9424)
                     /locus_tag="KPK67_000977"
     CDS             complement(9245..9424)
                     /locus_tag="KPK67_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457978.1"
                     /translation="MIHVEISEKEFFRKIEEVFDKGEGNIEVDNLGDIKIQVKKFDDK
                     TRSLELKLIKEFKLK"
     gene            complement(9429..9686)
                     /locus_tag="KPK67_000978"
     CDS             complement(9429..9686)
                     /locus_tag="KPK67_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457979.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSITKEKKDTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(9659..9799)
                     /locus_tag="KPK67_000979"
     CDS             complement(9659..9799)
                     /locus_tag="KPK67_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457980.1"
                     /translation="MVIAISLTLGLITGCTNKVSDTNEKIYTIEQISSIKKTCQRKYQ
                     NQ"
     gene            complement(9946..11338)
                     /locus_tag="KPK67_000980"
                     /pseudo
     CDS             complement(9946..11338)
                     /locus_tag="KPK67_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(11332..11573)
                     /locus_tag="KPK67_000981"
                     /pseudo
     CDS             complement(11332..11573)
                     /locus_tag="KPK67_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
     gene            complement(11890..12042)
                     /locus_tag="KPK67_000982"
     CDS             complement(11890..12042)
                     /locus_tag="KPK67_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457981.1"
                     /translation="MKNLNKNTILGEVIITIAACDDEKEIQIVIKNQVDNILKNKNKE
                     YRIKKI"
     repeat_region   12375..12865
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=12441..12469
                     /rpt_unit_seq="atttataactaacttagtgtaatttaaac"
     gene            complement(13144..15126)
                     /locus_tag="KPK67_000983"
     CDS             complement(13144..15126)
                     /locus_tag="KPK67_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG2457982.1"
                     /translation="MNNLYKYKAKSDGTSIDKHNEDMNYIMNQLVSIYNISNDMKNDL
                     YEAIVCHDVGKVVKSFQDNIESSTRRVRHELLSASIKYLNRGQKLAILTHHKDIKFLE
                     GQITNSIYEIELKELKELLDIEVEDIRPFIKEISRARNDDTRKLENILLKGYLQYCDH
                     IASAGIKSIDKGFNSIKDFSLPNGVSYNSIQEKVLNMDKNEDIMIMAMTGLGKTATSL
                     YWSNLVQNEYKSKRVYYILPYTASINSLYKNLYNRNISVSMLHSKAPYFLNRISDEED
                     IKQLYQIYKKSVKQINVCTIFQLVKSVFSCKRFEMLLSQLKDSIFIVDEIHCFDTRVL
                     CYTLEFLKYLKENLNVQICIMSASIPTCLKKLIQERLQISTIIEPDSKDLIFRHRLNR
                     LNEGILDSIHKIENDLMQGKRVLICVNSVDLSQELYGRLQKYNPKLIHGRFNTRDREQ
                     AEKGIEESFLLIGTQSIEVSLDISYDVLYTELAPLDSLLQRFGRVNRRGDNAELSNIY
                     IYDYDRKLNIYDEDIINRTDIVISDIIHNDNSIVDESKVPFYLDNVYLDIDMDKYNKH
                     KVALEIIMKNLRLGVWNKSANNDMLNNDTISVVPECLLEEYKLYINKKMYLEASSLFV
                     NVSVKRYIYNKKMFYECGDIVVTSYSYNDKLGLIFD"
     gene            complement(15137..16207)
                     /locus_tag="KPK67_000984"
     CDS             complement(15137..16207)
                     /locus_tag="KPK67_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HBG2457983.1"
                     /translation="MENKNSNRMLNIGYAIEVLCGRLNGSSSEVEKKNNSYKNSTYTK
                     KIGSRSRISSQCQKYNQKLFMEQVCGYEQNVKTKDGKQVKLVPNPFKYATDDIFGFMM
                     AEKLSIEEDVYKELSDEEKEIYKKNKKKYEANITKKRKSRWQMNSLINVSNRKVEWEW
                     NVCGTTGDSMPYQVEVYSGVHTNIANLNIGKIGEYDVSDVASEHRDYTTLEAKLIGVE
                     KLDKEEKFKRIEALLKSLEYLSVEGNKTNHLTDTKPKFVILGEYSWGNNVFQGIIKEN
                     GLDVEMLKESIEQNEEFRKSDIWIGINKFYDDTFNEIEVNNEKEQIKKYLEEEFEEYD
                     FMKISNVHQAFESYLKYLKETL"
     gene            complement(16228..17376)
                     /locus_tag="KPK67_000985"
     CDS             complement(16228..17376)
                     /locus_tag="KPK67_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457984.1"
                     /translation="MDRSYNVFVDNGLYVLAYYLNKDINDITYQDIENSIDLMSDKIE
                     EFVSCEKYSNLKSMCFSNSMLTQPKGKVTLNEKLKGFVKNQGDEYCSLCGQYKAKVKI
                     EDKEYNIGRSYMPNLVANTFYNFSNNLQGLNVCPYCLVLTMYSILNCRVSRYVFLYNS
                     TSDEFMKDYTCHIQDENLTDVELGAKKEKEKYSIVKLLESLVCKYNLFDGDIEQYMFN
                     NSGQSQDIQVNNIKNSDVNLLMKLQKEALLREFKDLHLEIYILRGTLESNYLRKIYNL
                     DKRERVASDELIVFLNREVNVLKKVKIDIIKKLTDKLKTSDLDIDKIYKELKMLGKFY
                     AYEDWLVNLNEMYYEKTGEKLFEAEDYIELDDIRSFRQIKNLILVSLI"
     gene            complement(17378..18142)
                     /gene="cas6"
                     /locus_tag="KPK67_000986"
     CDS             complement(17378..18142)
                     /gene="cas6"
                     /locus_tag="KPK67_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG2457985.1"
                     /translation="MHLVRRHLILTVDNRVVLDYNYQYELMKRIYEAIEVNDKRKALS
                     LHNEGYKVDKKVFKLFNYTIMFENAKYSKEGIHLNSQTKIKLILSGYDDILNNIIKGF
                     IKCKVFKLYNIEFKVSDIEEDSKKNFNNITLYKVRTPIVASLYDLKSRKQVYLNPMQE
                     EFYKALHDNLRNKYKLIYNKEYTGELYFDIEDVLAVKKKYITNIKGKGFVIGYTDFEI
                     FVQANKDMQEVIYYCGLGEKNSIGMGLLTYITSRRA"
     gene            complement(18133..18780)
                     /gene="cas5"
                     /locus_tag="KPK67_000987"
     CDS             complement(18133..18780)
                     /gene="cas5"
                     /locus_tag="KPK67_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBG2457986.1"
                     /translation="MILNKIEIIGDMAHFKIPMHSKIQRTYEIPPISTIVGILKNIYG
                     EEIDDFILGYVIYHNGTFKDLQTIYKEINPNVKTLTDNDRFQTDVCPIEYLINPKLII
                     YTNIDKNIEFNEPLCLGKTNCLAKVLSLSEIEVNLIDKKAIGYNQYTDINIGDGMIKR
                     INTLTEYNEKKGYYDIYSSMVRENDEFEYGKYYDEDEQQNIFLWNWKKGGVIKCI"
     gene            complement(19264..20391)
                     /locus_tag="KPK67_000988"
     CDS             complement(19264..20391)
                     /locus_tag="KPK67_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBG2457987.1"
                     /translation="MIIPKLEPNDRKREFNKYTSEQINKVVYNYLFKNNMSNRKIDEE
                     ILDLSSKQSKGYQSMGILHHLGINKNHKGIFRGYSTDKAIEFLKEDEIHFKQIINILL
                     ERKNINEYPIIVDSWELLDEKIALKTIDKSTLKHNGTGVPIEIRHFFEINNLGKNDKK
                     NVNFRYDDNYYSAVIEMDKLENPRSRIIWKSKFRDLLRNIFPKYYEKFLEGKIEENTN
                     LPKIRFVKFDSNNFDIEFIFTSEIEDDVLEEISQDNPLDIDTINIELETKIRKEGKVK
                     YVYGKQYERNPKNRIEAIKYHGTKCVVCGFDFEKIYGNRGKGYIEIHHIRPLSFVREE
                     SNINPKTDLVPICSNCHRMIHRKKDSVLSIDELKKIIKDSK"
     gene            complement(20665..20937)
                     /locus_tag="KPK67_000989"
     CDS             complement(20665..20937)
                     /locus_tag="KPK67_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457988.1"
                     /translation="MYDEIIEIYFDYWINEHMYRDILEKDEYYIEGWKEVEKINELLE
                     KSLEDDKKCVLVELNDLFNSLIEFERELSYKLGFKAGAKFIIELLK"
     gene            complement(21185..22519)
                     /locus_tag="KPK67_000990"
     CDS             complement(21185..22519)
                     /locus_tag="KPK67_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2457989.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVQELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFMSY
                     VSIVIEKYKNRNIIDKFNKFSFGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEVNEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLDDEEIVKYIQALSIINQENNLY
                     INDSVNTISGIKREIKSIKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQFAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            complement(22532..23275)
                     /locus_tag="KPK67_000991"
     CDS             complement(22532..23275)
                     /locus_tag="KPK67_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457990.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITILLMASHKENHV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLFFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSRIREYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMSSSNFTK
                     ILEGNYHSKEIVINKETGTYNPDDSQLKW"
     gene            complement(23297..23611)
                     /locus_tag="KPK67_000992"
     CDS             complement(23297..23611)
                     /locus_tag="KPK67_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2457991.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASRLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            complement(23615..23815)
                     /locus_tag="KPK67_000993"
     CDS             complement(23615..23815)
                     /locus_tag="KPK67_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2457992.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKED"
     gene            23962..24726
                     /locus_tag="KPK67_000994"
     CDS             23962..24726
                     /locus_tag="KPK67_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2457993.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYFLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDI
                     KEIIKNDVLTKQSIDELLDIGLSEENIETIYDYFSDSTDNGSNIRLKKLISQKENITI
                     KNNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            24783..25535
                     /locus_tag="KPK67_000995"
     CDS             24783..25535
                     /locus_tag="KPK67_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG2457994.1"
                     /translation="MNKSYIPKYTYKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKEKLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     GDEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            25525..26691
                     /locus_tag="KPK67_000996"
     CDS             25525..26691
                     /locus_tag="KPK67_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2457995.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIEGHKLEVLFLVALGTGLRLGELLGLKW
                     FDIDFKNSNLTVKRTLQRTYFIDKNGDRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNTDILKAGELYRNDNYVFCNELGVPVDDKRPLRNLKSILNSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(27649..28563)
                     /locus_tag="KPK67_000997"
     CDS             complement(27649..28563)
                     /locus_tag="KPK67_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HBG2457996.1"
                     /translation="MELVKNIHKNKSKDEIFKELLENLKGTIASWDYYVNWNKALENT
                     RKIEVQLNILNYLIGKEDIENETKNLLKEYPEVLSVIPIVIASRDNEFKILDPSDEDM
                     FKYKLFNFKKRKSISDEESELATEFLKKIKFLDLLKQKNIKNLVDYVFGVEVGLDTNA
                     RKNRTGSSMETLVERYVKNMCEKNNWSYLTQATVKKISERWSIEVPTDKSKRKYDFAI
                     NNNGKIVLVEVNFYNGGGSKLKSVANEFTKLDKYIKDGGFTFCWITDGLGWLESSIPL
                     RDAFDKMDYIFNLDMVDKGSLEYIIKNL"
     gene            complement(28582..29496)
                     /locus_tag="KPK67_000998"
                     /pseudo
     CDS             complement(28582..29496)
                     /locus_tag="KPK67_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
     gene            complement(29923..30272)
                     /gene="ssrA"
                     /locus_tag="KPK67_000999"
     tmRNA           complement(29923..30272)
                     /gene="ssrA"
                     /locus_tag="KPK67_000999"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(30430..30885)
                     /gene="smpB"
                     /locus_tag="KPK67_001000"
     CDS             complement(30430..30885)
                     /gene="smpB"
                     /locus_tag="KPK67_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBG2457997.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            31449..32276
                     /locus_tag="KPK67_001001"
     CDS             31449..32276
                     /locus_tag="KPK67_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG2457998.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
BASE COUNT        10765 a         5261 c         3260 g        13105 t
ORIGIN      
        1 ttattattct aataaaaaca actatacata gattaaaatc accttctaaa cataattatt
       61 aaaatttaat taatgatatc aattccatta atctctttta ataaaaaatt ctcttttcta
      121 tatgaaaatt atttcaaaac tttaaagtta aatacaaaaa tgactataaa tagattttta
      181 tttacacaaa tctatctata gtcactatag tttatccaat aaaataataa aataaatatg
      241 tacacaataa atgttattta cgtcttaagt atttatttgt tagattttta attaaaaaaa
      301 acaagctaat gaacattaat gctaaatcta gcattatatt gctttgatct cctgtttgta
      361 cattttgacc tacattacta tctaaacttt catctgtatc tatatttata tccggctttc
      421 catccccatc tgtatctata tttatatccg gttttccatc tccatctata tctatattta
      481 catccagctt tccatctcca tctatatcta tatttacatc cggctttcca tctccatctg
      541 tatctatatt tatatctggt tttccatctc catctgtatc tatatttata tctggtttgc
      601 tatttcctcc attatctggg tctggtttac tatttccacc gttatctgga tccggtttgc
      661 tatttcctcc attatctgga tctggtttac cattgccacc gttatctgga tctggtttgc
      721 tatttcctcc gttatctgga tccggtttgc tatttcctcc attacctgaa tccggtttgc
      781 tatttcctcc attacctgaa tctggtttgc tatttcctcc attacctgaa tctggtttgc
      841 tatttcctcc attacctgaa tctggtttgc catttcctcc attatctgga tccggcttgc
      901 tatttcctcc attatctgga tctggcttat ttagatttgg attacctgta tcataacctc
      961 ctgagccagt atctacattt ttatcaggct tccaataatc atctactcca tcattatctg
     1021 tatctattgc tggacgatta taatctgtat cagggtttcc atctccattg gtatctatat
     1081 ttttatcagg tttccaaata ccatctccat ttccaccttc tgtactaggc ttccattcac
     1141 ctgtattatc agtatctatg ttaatatctg gcttccaatc accactcgta tctatattga
     1201 tatctggcct tccatcacca tcagtgtcta tatttatatc cggtattcca tccccatcta
     1261 catctacatt tgtcacatct aactttaact tcttttcatc ctcactacct tcaatgtaat
     1321 ttatatcatc tccaatatat tttacagtaa tagtttgttc agtagtaaaa atatgatgat
     1381 tgctatctaa aggtctccat tcaatagatg cagtactata attatgactc tcactaccct
     1441 tattatgagt ttttaattct actggatctt tatattcttt tccattaata tagaattgta
     1501 cgtaacctct tggagaggca caatctggtc cttctttctt atctggtgaa atagtaacat
     1561 ttaatgtaaa cgtatctgtt ggtttaaaaa catcagtttt agctttagac tcttctacaa
     1621 ctaatgaagt ttctgtatca gcaggaataa tttctgcctt ataatatgct ctatgccccc
     1681 aataagcttc attaaaagga tatacatttg aaaactgtgt tgatgtaatg cttaattgat
     1741 attttccagc atcaacttgt tttgtaaggt catcacttcc taaatttgta tctggttcca
     1801 ttgtttcctc atttaaacgt tgagaactaa tagtcataaa ttttttatct gtgagttttt
     1861 cacctttagg agtaattatt ggaatacccc catcttttaa tatccaatct aaaaatgatt
     1921 tttctacatc ttcttcaaat ggtaatccat catatctttt aataatcaaa tatggtggag
     1981 tatcatcaat agtaaattct tcatattgct ttaaaaattg agaacctgaa cctatatctt
     2041 taacataaaa atcatctggt tttatatttc ctccagtaat tttatctata acaactaaat
     2101 taacagatac ttcagatccc ttaaattgat cagaaaacca taagtaaaga accccttgat
     2161 catctgtata actaggtgct ccataaggat aaatttttcc atcaattagc attttaaaat
     2221 catcaactaa tgtagtacta actttatctt gtcctaaata ctttaaagat attttattca
     2281 taaaaacttt tgttttcttt tctaaatcac cgtatgcaac tgctctacct aaacttttga
     2341 atgttgttgc ttttaatgaa ccacctgtta ctataacatc tcccgttgta gcacttaggt
     2401 caaaaatcca atctcccact cctgtcatat aaggtaaaat cgttcctcca gttacaagta
     2461 tacgatatcc tctatttgtt cctggattaa cacatccatc cccaaatgca ctaccatgtg
     2521 cacctccata tgaataggaa agtccaccat taattgtaat atttccacta taaccagaac
     2581 ctgtgctagg atctactact gagggactta atgcattctt tgttgttgta tagtatcctc
     2641 cacctattcc agctccatgg tatgcacctt tagcagttat agtaccacca ttgattgtga
     2701 tatctccacc actaccatta ccgcctccac ctattccagc tccatatcct gtattacgac
     2761 tcgattcttg acctatagca gttatagcac caccattaat aataatattt cctccacttt
     2821 cagtatttcc accgccaata cctgcagaat aataatttcc aacaactttt aaacttcctc
     2881 tattattact atctagtagt gtaaggcgat ctcctttttt taacttgtct ccattttcta
     2941 gagttacatc tctaggaatt tttccacctt caggaactat aaactctcct tcaatatctc
     3001 tatttacaac agaatcatca ataattagag tagtaccttt tcctgctctc atacctgggt
     3061 aatatacatc acctgactct agcttgtttt cactaccatc ttgtaatata acatgtgcat
     3121 atgcaccacc atctttatct ggtgttaaag taaatgggct attagatgca gttttaatat
     3181 ttactccatc aaatattaat tcagctttta tatctttagg tataacaatc ctatgtgttg
     3241 ttggagtatt tggattatta ttttgaattg tcatttgttt tccactaaga ataattaaaa
     3301 cgccacttgc aaatttaaaa tcgacattta tttctccacc actaatttta aagtctccta
     3361 cagatgtagg atcttctgca gtggcttctt ccacaactaa tgcattcata tcttcttctg
     3421 catttcctgc attagcatct ccttctgcat ttcctgcatt agaatcttct tctgcatttc
     3481 ctgcattagc atctcctcct gcatttcctg cattagcatc ccctcctgca tttcctgcat
     3541 tagtatctcc ttctgcattt cctgcattag tatctccttc tgcatttcct gcattagtac
     3601 tttcttctat agtatctgcg aaagtatttg acgcattaaa tgatgctact gagccaagtc
     3661 ccattgagat acttaaaatc attgatagtg ctttcttttt acatttttgt tgcataaatt
     3721 ccccctcccc ttaataattc ttaatataca ctgtatacat ccttatatac actctaataa
     3781 ttcttaatat acactgtata catccttata tacactctaa taatttttaa tatatactta
     3841 aaacttatat ttgtcaatga atatactgtt tttttcaacc ttatatatta taagtattca
     3901 ttatttgcta aataactaca ataaaaattt taattatata aaatttattt aattttttat
     3961 ataataatac taaggggtaa ttaattgaaa aaactagtaa aaatattaat aaatattatt
     4021 tgcattattg cgctaacttt ctctagcctt tcgatttaca taaaattatc tgaatataaa
     4081 aaagctgatg aagtatatac ggaactcaga gaaaacacta tcaataatag caaacatcaa
     4141 gaactttatg ataaaaataa tgattataga ttctggctta aaataaacaa cgtaaatata
     4201 gactatcctg tagtacaagg atataacaat gatttctacc taactcatga tttttataaa
     4261 aattatcttc ctttaggtag tattttcatg gattatagaa ataattttga aaatgataag
     4321 agtttaatag tctatggtca ttatatgaaa aataaaacta tgtttggtca gctagaaaac
     4381 tatactgatg aagtattttt caaagaaaat aacttagtag aaataaatta taaagtacaa
     4441 acttatactt atgaaatttt ttcagtatat actgctgact taattaacag agattattta
     4501 agtatacact ttaatagtaa ttatgagttt aaagattatt tgaattatat aacctaaaaa
     4561 tcagtacaca aatctgatat aaaggtagat atttcagata aaattataac tttgtatact
     4621 tgtaactatg aatttaaaga tgctcgtact atagttcatg ctaaattaat ttcaaaaaaa
     4681 gagactgata agtaggtttg tgtaaatgca gacttaccta cagtcttttt tgaacgtaaa
     4741 attggtaagt atggaataat atttatatta aacttttgta ggcaaagaaa tgaaaaatca
     4801 aagagaaata attattacag atttagatta ccaagctgaa ttaagaaagt gtaaaactat
     4861 ggaggatgta gttggtgaag ataatatata atagtttaac ttataaatta tcttggtgat
     4921 attaatgaag tttcaaagta tatattattt attgatataa ataaaattaa atctgtttct
     4981 aatagatgta cctttgtttg gagacaaact attgaagaaa tataactaaa atgttgaagt
     5041 agctacctga ctttattttg aaatgcgaac aagattttgg cattaagata atttagaaag
     5101 aatcactggt acacaaggat gtgaacttag aatgaataga agtattcagg cataaggagc
     5161 tttcacccaa ataaaacata gtatgaactt taaaagaatt ctaatcagag gcaatcaaaa
     5221 tgttctttca tagtttatac ctgtagtgtt agcccatagt atttctaagt ttcacaaata
     5281 agataatcat taataaatgt agtatgtata ttcatcctct caaaaatatt ttttaatctt
     5341 acactttgta aattattcta ttttaaaatg gtatttttat acctttattt cttatgaccg
     5401 ttttcaagct caaattttgc ataaatataa attaatgaaa agcaagtaaa aaaagaactg
     5461 tcgcacagca atttaaaatt actcattcga cagtccttat ttcgtggatt taaatagtgg
     5521 agaagaatca caagaataat acccacgatt aaacctttat acaatacatt ttatatgact
     5581 ttaccatcac taatagttat tatacaatca gccatcttag ctatttcatc attatgagtt
     5641 atcataacca acgtctgctt gtattcattt acacaatact ttagtaaatt catcacctca
     5701 ttagttgttt tactgtctaa atttccagta ggttcatctg caaaaattat tgatggctta
     5761 ttggcaagtg atcttgctat tgcaactctt tgttgttgac cacctgataa ttcattagga
     5821 aatttattaa tctgactttc taatcctaac ttagttatta aatcattaat ataattatta
     5881 tctatctttc ctttagcaat taaaatagga agaactatat tatcataaac attgataaca
     5941 ggtattagat tatagttttg gaatataaac ccaaattctt tacttctaag attagatagc
     6001 ttttcatcac ttaaagtata taaatctaaa ttatccataa atacattacc taaagttggt
     6061 ttatcaagcc ctgccataca gtgaagtaat gtgctttttc cagaaccact ttgtccaatt
     6121 attgcactaa atttatgggg ttctatctct aaagtaattc catcaacagc ctttatttca
     6181 ttttctcctt ttccatatat ttttttaata tctataactt ttaacatctt catacattta
     6241 cctcccaaat tattcattct cttttatacc ttcaataata ttcatacttt caaaatctct
     6301 attacttaca actactgcta ttaaacatac aaatacagat atagcaaaga atagtaaaga
     6361 ttccttaatt gggaatgtaa aagacataaa attctctatt ccatagttta tatacgcatc
     6421 attattccaa tttgtaaatt taactagatt tataaatgat ggaatcatag ctattataaa
     6481 gctaaaaact gcatatgcta atgcttcata tataagcatt tttttcatat ttttaagact
     6541 aacacctaat gctctaagcg ttgaaatttc tttttttcta attagtagat ttgttcttat
     6601 agtacagaaa atattaattg atgctattat taaaactagt gcaattattg ctatttgtga
     6661 tttaatgtaa cttttatata tactttctga catttcatga taacttaatc tactattaaa
     6721 tagagaccct ggtatacttc ttgaaatttt ctgtatctca ttattaacaa tctcatcata
     6781 tggattttct aaatttatgc ctaattgagt gtatttttgt tctcctgtta tgtttttaga
     6841 gtgaatatta cttgttataa tttctagatc tggcataaaa tcgccatctc ccatagcaat
     6901 ccattcatcc tttaaaatta cagccactct taccttcatc tctttataaa caattgtatt
     6961 attttcttca acaggaattt ttacagttat tatgtctcct atatttaatt ctttgaatat
     7021 aggctttata gtatgatcat caactatatc ataaaaataa ttatatactg ctacatttgg
     7081 atatccatca gtatcctttt taatatcatc taatctacct tcttcaacaa agtcatttaa
     7141 cgtttcatct atataatcat catcataaag ctttaaaact aatttatctt ccattatctc
     7201 ttttttttct actccattat aggttgtaaa ttctttgttt atatccttat tattaaaagt
     7261 aaacattcca tttgttattt gaattttatg tatactttta acacccttaa ttttttctat
     7321 attctctaca tcactatccg tgtattttga tatttgcggg tctgcattta ctccaaattg
     7381 cattgttata ttatatctat tttgcatatc ttcaatttta ctgttgacct cttcttgcat
     7441 acttgagaat gtagtaatga atatatatcc tcctaaagca atagacaaca tagatatagc
     7501 agctctaacc ttatttcttc cgatattttt aagtcccata aatccataaa atccaaatat
     7561 tttgcttact agtctgtcta ttccaagtct acttctgtga tttttaattt tgtctgtatt
     7621 tattattgat tcaataggtg atatcttgcc gattttaaaa gttggtataa tacaagatat
     7681 aaaaacagaa attattccaa ccaatatagc ttttataact atttcattat ttatatataa
     7741 tttagggtta agaccagctt cactgtataa aacttcgtta taattactaa ttcctatata
     7801 tgaatatgta accccagcta ctaacccaag tagtatacct attattgtta taattaatgc
     7861 ttgatagatt atcatacacc ttacgtttct tttctttgca cccatcagtc taaacagtcc
     7921 catttctttt actgattgat ttagtgtaat attgaaaata ttaaatatga aaattataga
     7981 tactgtagtt attaaaacat ttcttttaaa aacattcgga tcattcttat catcttctac
     8041 ataacgaagt tcccttacaa gaggtacatt tggtagaaag cttttttgtc ctaacttata
     8101 ttttttcata agataatcac attcaccttc aatatttctc catccacttt taaaacttaa
     8161 tatactatta taactaatcg catcattagg tataatgttg tttgtttcat cattgccata
     8221 agttactgct tcgtattgcc aaaccacatc atagtaccct tttggttttt ccataactcc
     8281 tactatttta aattctttat ttatagaaaa taatcgatta cttccgttag tatctttata
     8341 gttctttttt attgttaaat ttaatgtaga tcctactcct tcacttaaat tcattgcttc
     8401 cagtgctttt ttctctaata ctatttcatt gttgttttta ggtagtctac cttctattat
     8461 tttgttgtat ctacttttta gatactcttc gtttgatgaa gttaatataa atgagtttcc
     8521 ttttttatct gaaatacgcc ctagattttg taccaaatta ccctttgata ctttttcatc
     8581 agtttctaat tttttagcat catcaagtgt caaatcacta aactctacat ggtatccact
     8641 gtaaagttca tgtattgcta ctacattgtt ttcattttgt gaatctttta taacactagg
     8701 tccaaatatc aacataacgc ctaacataac tcctattatt aaaagtagtg ttctaagttt
     8761 atttttcttt aaatatgcta gtgacatttt taagtaaatc cccatataat cctcctattt
     8821 tactataaat tatttatcca actcatctgt taaatagatt ggtggatatt ttaaatcttt
     8881 taattcttca ttattttttg aagcttacta ccatcttcct catgataaac ttgtagtatc
     8941 accttattta catgtagtgc ataataatca ccactataaa aatattagta tttataacct
     9001 ggatagtttt tgtaaaatga tttataaagt atcccatcct aattctcttt tgtatacttt
     9061 tcttttgtaa tacgctctat atcatcacct gaaactcctt gatatgtcat atatttatct
     9121 atatattttt cagcttcatt ccattttcta aaatagttgt tgtatataat aaatcctatt
     9181 atggctataa tgcttataat taaagttaat aaaatagctt tgtattttct catagtgaat
     9241 cttcctactt caatttaaat tctttaataa gttttaattc taaagaccta gttttatcat
     9301 caaatttctt aacttgaatt tttatatctc ccaaattatc aacctctata tttccttcac
     9361 ctttatcaaa tacttcttca atttttctaa aaaattcttt ctcacttatt tctacatgaa
     9421 tcaatgatct atatgttata aatggccatt atgaatttat catcttctga cagcttttca
     9481 ttataagaaa aatctttaac acattgaatt gataatattt ccttcgtatc cttcttttcc
     9541 ttagttattg aataatgtaa aacttggcta ggttgttctt ttgtatactc caagtttccc
     9601 aaagacaaaa caaaacttcc atctgattta ttttcagcta ccctaagacc actattttct
     9661 attgattctg atattttctt tgacaagtct tcttgatact agaaatctgt tcaatagtat
     9721 atattttttc atttgtatct gaaaccttat ttgtgcatcc tgttattaaa cctagtgtta
     9781 agcttatagc tataaccata gttattgtaa tttttttatc ttcatataat cctccttata
     9841 tattgaaaac tcgttatttc tcataaaaaa aagacgctat ctttttctat taaaagataa
     9901 catccctata tgtttagtat caatatatgt tccccaactg gtaggttaat cgacccaact
     9961 atcaaaattt tgatttaatg gtatataaat attcaatata aatttgtctt ttttatcttc
    10021 aaataataaa tctcctctat atttttttat agaagatttg atgctttaaa tccctattcc
    10081 atgtacaagt ttatcttttt atctgttaat aataaacttt tattctttct aattgaattt
    10141 attttactat tctcacattt aattacgaaa taggactttg agatagttcc ttttatttgt
    10201 atatatttat caatactttt atcatgtatc ttatcgcaag cttctattgc attatctaag
    10261 tatttgcaaa tatattagat acatctattg gttttataaa attcactttg gaaaaattga
    10321 tatcacaaat gaatttaatt tttctttttt cacttacatc acttttttca tttattatta
    10381 tatccaatat aacattacct gtattgtaaa ttgatttaaa ttctttaatt taatctttta
    10441 aactatttac atacccatca attttccttt tttatatttg agattttcta tacaatatat
    10501 atgattattt atatcatgat aaagcttctt aactttagcg tgtgattctt gaatacttag
    10561 ataatgagaa tactgcatat ctagcttatt ttttattact tcattttgca attttaactt
    10621 attttcctta attattttta taaacactat aataatgact aagctactag ctagtattaa
    10681 cctaggtatc acaatatacc caactatata tatttcctaa tacccaccaa atcgatacat
    10741 ttttctattt atataaatta ttaatatagt aaataaattt gttattaaag caacagataa
    10801 gtatatatag tttattttat tatctttatc tagtcccaat cttttattaa taaatttaaa
    10861 tatagcacaa gctactatat aactattatt ataagataat agtatatatc tactttagca
    10921 ttatcataac taaacactaa tagagcttcc tctgatgata ttacattact tgtaactaca
    10981 aaaaataaat ttatagatat aacatctttt attaaagggt gatatacgtc agataagaaa
    11041 aatgaaacta tatatttaac aattcctatc ttataaaaat aattatagta agttcccaag
    11101 taataaatat caaataatag attatatcat atagattttt cttcatatta taatcatggg
    11161 tcttatcaac gttaaataat ataatgattt tagttataag ataagctaat aatacagtaa
    11221 aaaaagatat tttcttagtc ttatctgtat ctttatcgta agttaatata ctttttagta
    11281 tcattattgg tgtaaacaat gataatatta ataatatttt atttatatgg tttaacatat
    11341 tctatcacca ataaatttta gaaacccctc ttttatatct ttatatctat atctactaat
    11401 aggtacttct tcttgactgt ctaatattgc agtatttgac tttatatttt ctacatagtt
    11461 taagttaatt atataacttt tatgacatct tacaagttcc tctatattta aatctttttc
    11521 tattttctct tacatatagc atcaaaagtt ttattttttg tatgtattag catgttcttt
    11581 ttttgaactt ctatatattt ttttgctaga gtagattttg tatgtgttag acttattttt
    11641 atttttttca atttttttga tatatgttaa tatgtgtttt tctagttctt gaaaatttat
    11701 tggctttaat aagtatcaat aagctcttac ttcatatctt tcctgtacat aatttattag
    11761 agatgttata aatattatta tctacttttc ttatgtttct tgcgatatct atactattta
    11821 gttctggcat ttttatatct aagagtagtg tatcctctca attaactttg taatggttct
    11881 tcttttgagc tatatttttt taattcgata ttctttgttt ttatttttta atatattatc
    11941 aacttgattt tttattacga tttgtatttc tttttcatca tcacaagctg ctattgtaat
    12001 gataacctct cctaatattg tatttttatt tagattcttc actttaggtt aacacttttt
    12061 aaatgtattt atcaataata tttaaattaa aaatgtataa aaatacctat atccaattgg
    12121 atataggtat tcgtggattt caatggtgga tacgagggga gtctataagt ggttatttga
    12181 ttgttttgtt gatttttgta aatataggta tatagctatt tggttatgat gttgaagcaa
    12241 tttttttagt tattttttat tgtttatttt tgtagttggg gtattattgg ggtataattt
    12301 tctactttcc aaaattattg atacttcaat aatataaatc aaattcttag ttaagttttt
    12361 taatgaatag gtaaatttac gactaattta gtgtaattta aacaagacga tgatttagaa
    12421 aatagtttga aaaattctaa atttataact aacttagtgt aatttaaacg agcttaatac
    12481 attagctatg aaaagcagaa taaacattta taactaactt agtgtaattt aaacattcca
    12541 agacttgaca tagagtcagt tactaaatct gatttataac taacttagtg taatttaaac
    12601 aagtaaattt tacgagtgga taaagttgct tgaaagaaaa tttataacta acttagtgta
    12661 atttaaacct taaaccttgc tttaaatttg tagtatccaa agcagattta taactaactt
    12721 agtgtaattt aaacattaga aaaaataata aatagaggag gcatatgatt atttataact
    12781 aacttagtgt aatttaaact tataacctgc gacaaaagtt ttgtgttgac ctttttattt
    12841 ataactaact tagtgtaatt taaaccccaa ataaaactat agtaattcca acacttgtaa
    12901 ggttattaaa ataagtaatt tgcagtgagc caccagtagt gtttttagct aaaaataaaa
    12961 atacagttat aaaccttgcc atcactagct cacagacatt cacacatatt tttcgctcac
    13021 tgcaaaatta ttctactata acatataaaa cttgtagtag atataaatta taacaaattc
    13081 tcaataaaat attttaatta tttatattat aattctctta acaatcaaga tttaattaaa
    13141 aatctaatca aatatcagac ctagtttatc attataacta taacttgtaa cgactatatc
    13201 tccacattca taaaacatct ttttattata aatgtatctt tttacagata catttacaaa
    13261 taatgaactc gcttctaaat acattttttt attaatatac aacttgtact cttctaaaag
    13321 acattcaggc acaacactaa ttgtatcatt atttaacata tcattattag cagatttatt
    13381 ccagacccct aatctcagat ttttcattat aatttctaaa gcaactttat gtttattata
    13441 tttatccata tctatatcta aatagacatt atctaaataa aatggaactt tactctcatc
    13501 aactatagaa ttatcattat gtattatatc tgatattact atatcagtcc tatttattat
    13561 atcttcatca tatatattaa gctttctatc ataatcatat atatatatat tgctcaactc
    13621 agcattatct ccccttctat ttactctacc aaacctttgt aacaatgaat ctaaaggtgc
    13681 taactctgta taaaggacat cataacttat atccaaactt acctctatac tttgagtacc
    13741 tatcaataaa aagctttctt ctatcccttt ttctgcctgt tctctatctc tagtattaaa
    13801 tcgaccatgt attaatttag gattatattt ttgaagcctt ccatataact cttgagataa
    13861 atctactgaa ttaacacata ttaatactct ttttccttgc attaaatcat tttctatttt
    13921 atgtatgcta tcaagtattc cttcatttag tctattcaat ctatgtctaa atattaaatc
    13981 tttactatct ggttcaataa ttgtacttat ttgcaatctt tcctgtatta acttttttaa
    14041 gcaagttggt atacttgcac tcattataca tatttgtaca tttaagtttt cttttaaata
    14101 ttttaaaaac tccaatgtat aacacagtac tcttgtatca aaacaatgta tttcatcaac
    14161 tataaatata ctatctttta attgtgataa tagcatttca aatcttttgc aggaaaatac
    14221 tgactttact aattgaaata tagtacatac attaatttgt tttacactct ttttgtatat
    14281 ttggtaaagt tgttttatat cttcttcatc actaattcta ttcaaaaaat atggagcttt
    14341 actgtgtaac atacttacgc ttatatttct attatataaa tttttatata aagaatttat
    14401 acttgctgta taaggaagta tataatatac tctcttactt ttatattcat tttgaactag
    14461 gttactccaa tacaatgatg ttgctgtttt tccaagacct gtcatagcca ttatcattat
    14521 atcttcattt ttatccatat ttaagacttt ttcttgtatt gaattataac ttacaccatt
    14581 tggcagacta aaatctttaa tagaattaaa tcctttatct atacttttaa ttccagcaga
    14641 agctatatgg tcacaatatt gcaaataacc ttttaataat atattttcaa gttttctagt
    14701 atcatcattt cttgctcttg atatttcctt tataaatggt cttatatctt ctacttcaat
    14761 atctaatagt tcctttaatt cctttaattc aatttcataa atagaatttg ttatttgacc
    14821 ctctagaaac tttatatctt tatggtgagt taatatagct aacttctgac ctctatttaa
    14881 atacttaata cttgctgata ataactcatg tctaactctt cttgtgctac tttcaatatt
    14941 atcttgaaag ctttttacta ctttccctac atcatgacat acaattgcct catataaatc
    15001 atttttcata tcattagata tattatatat actaactaat tgattcatta tgtaattcat
    15061 atcttcatta tgcttatcta tacttgtccc atctgattta gctttatatt tatataaatt
    15121 attcatgata tatcctttat aaagtctcct ttaaatattt taagtaactt tcaaaagctt
    15181 gatgtacatt tgatattttc ataaaatcat actcttcaaa ctcttcttct aaatacttct
    15241 ttatttgttc cttttcatta ttaacttcta tctcattaaa tgtatcatca taaaatttat
    15301 ttatcccaat ccatatatcg ctttttctaa attcttcatt ttgttctata gattctttta
    15361 acatctctac atctaagcca ttttctttga taataccctg aaatacatta tttccccatg
    15421 aatactctcc caatataaca aattttggtt ttgtatcagt taaatgattt gtcttattac
    15481 cttctacact taagtattct agtgatttta ggagagcttc tattctttta aacttttctt
    15541 ctttatctaa cttttcaaca ccaattaatt tagcttctaa agttgtataa tctctatgct
    15601 cacttgctac atcagataca tcatactctc ctatctttcc tatattaaga tttgctatat
    15661 ttgtatgtac tcctgaatat acctcaactt gatatggcat actatctcct gtcgttccac
    15721 aaacattcca ctcccattca acttttctat ttgatacatt tattaaagag ttcatttgcc
    15781 atcttgactt tcttttttta gttatatttg cttcatattt ctttttattt tttttatata
    15841 tttctttttc ttcatctgat aattctttgt atacatcttc ttctatagat aatttttccg
    15901 ccatcataaa cccaaatata tcatctgtcg catatttaaa tgggtttgga accaatttta
    15961 cttgtttacc atctttagtt ttaacatttt gttcataacc acaaacttgt tccataaata
    16021 gcttttgatt gtatttttga cattgacttg agattctact tctgctccct atttttttag
    16081 tataagtaga attcttatat gaattatttt tcttttctac ttcactacta cttccattta
    16141 atcgcccaca taaaacttct attgcatacc ctatatttaa cattctattt gaatttttat
    16201 tttccatttt aaccctccat aatattatta aattaaactt actaatatta aatttttaat
    16261 ctgtctaaag cttcttatat catccaactc aatataatcc tctgcttcaa atagtttttc
    16321 tccagttttt tcataataca tttcatttaa gttaactaac cagtcctcat atgcataaaa
    16381 ttttcctaac attttaagct ctttatatat tttatctata tctaagtcac tagtttttaa
    16441 tttatccgtt aattttttta ttatatctat tttgactttt ttcaatacat ttacctccct
    16501 gtttaaaaat acaataagtt catcagaagc tactctctct ctcttgtcca aattatagat
    16561 ttttctaaga tagttacttt ctaaagtacc ccttaatata tatatttcta gatgtaaatc
    16621 tttaaactct cttagtaatg cttctttttg taatttcatt aacaaattta catcactatt
    16681 ttttatgtta ttcacctgaa tatcctgaga ttgaccagag ttattaaaca tatattgctc
    16741 tatatcacca tcaaataagt tatatttaca tactagactt tctaataatt ttacaattga
    16801 atacttttct ttttctttct ttgcccctaa ctctacatcg gttaaatttt cgtcttgaat
    16861 atggcaagta taatctttca taaattcatc tgatgtagaa ttgtataaaa aaacatacct
    16921 actaacccta caattcaaaa ttgaatacat agtcaatact aaacaatatg gacacacatt
    16981 caaaccttgc aaattattgg agaaattata aaatgtattt gcaactaaat taggcatata
    17041 acttctccct atattgtatt ctttatcttc aattttaact ttggccttat attgaccaca
    17101 taaactacaa tattcatctc cttgattttt aacaaaacct tttaacttct catttaaagt
    17161 aacttttcct tttggttgag ttagcataga attgctaaag cacatacttt ttaaattaga
    17221 gtatttttcg caacttacaa attcttcaat tttatcactc attaaatcta tactattctc
    17281 tatatcttga taagttatat cattaatatc tttattcaga taataagcta atacatatag
    17341 accattatca acaaatacat tgtaacttct atccatatta agccctccta gaagttatat
    17401 aagttagtaa ccccattcct atactatttt tttcacctaa tccacaataa taaattactt
    17461 cttgcatatc tttattagct tgtacaaata tttcaaaatc tgtatatcca attacaaatc
    17521 cttttccttt tatattagtt atatatttct tttttactgc taatacatcc tcaatatcaa
    17581 agtatagttc accagtatat tctttattat atataagctt atacttattt cttaaattat
    17641 catgtaatgc tttataaaac tcttcttgca ttggatttaa gtatacttgt ttcctacttt
    17701 ttaaatcata taaactcgct acaataggtg ttcttacttt ataaagtgtt atattattaa
    17761 aatttttctt tgaatcttct tctatatctg aaactttaaa ctctatatta tataatttaa
    17821 atactttaca tttaataaaa ccttttataa tattattcaa tatatcatca tatccactta
    17881 aaattaattt gatttttgtt tgggaattta aatgtattcc ctcttttgaa tacttagcat
    17941 tttcaaacat aattgtataa ttaaataatt taaatacttt cttatccacc ttatagcctt
    18001 cattgtgaag gcttagtgct tttcttttgt cattaacttc tatagcctca tatatccttt
    18061 tcattagctc atactggtaa ttgtaatcta atacaactct attatccact gtgagtatca
    18121 aatgtcttct cactaaatgc atttaattac tccccctttt ttccagttcc ataaaaaaat
    18181 attctgttgc tcatcttcat cataatattt accatattca aactcgtcat tttctcttac
    18241 cattgaagaa tatatatcat aataaccttt tttttcatta tattcagtca atgtatttat
    18301 tctttttatc attccatctc caatgttaat atctgtatat tggttatatc caatagcttt
    18361 tttatctatt agatttactt caatttcact taaagataat accttcgcta aacaattagt
    18421 cttacctaag catagtggct cattaaattc tatattttta tctatatttg tatatatgat
    18481 aagtttagga ttaattaggt attcaatcgg acatacatca gtttgaaatc tatcattatc
    18541 tgtcaatgtt tttacatttg ggttgatttc tttatatata gtttgcaaat ctttaaatgt
    18601 accattatga tatattacat atcctaatat aaaatcatct atctcctcac catatatatt
    18661 ttttaatatt ccgacaattg ttgatattgg tggaatttca tatgttcttt gtatcttgct
    18721 atgcatagga attttaaagt gagccatatc tccaattatt tcaattttat ttaatatcat
    18781 tttatctcct caaatattta gttaaaataa tccattttat ttataaaatt agacattcat
    18841 ttataaaaac ctttaatttt taccattatt ttcctaaaag taacaaatac ataacttaat
    18901 tagattattc tagtgaattt tctaaaaact ttgcacctac cttaaatcca agttatcctt
    18961 caaactttca atataatact tgtaattttc taaaatattt ctgtatatgt ttcatttatc
    19021 taacaatcaa aatatatttc tatggattca tcatacatgt tagtacttaa ttgattttca
    19081 tcaggaactc aagatatgcc taattcatta tactaatata ccttaaatat ttaatttata
    19141 aaggtagctg tatacttata tagcaactac cttttcctaa taaacttttc aattttttat
    19201 attagtattt attgttactt aatttagagt atatacttta tgcatctcta cctcacataa
    19261 gtattactta ctatctttta taattttctt caactcgtct atacttaaaa cactatcttt
    19321 tttcctatga atcattctat gacaatttga acatattggc accaaatctg ttttgggatt
    19381 tatattgctt tcttctctga caaaacttaa tggtctaata tgatgaattt ctatgtatcc
    19441 cttacctctg tttccatata ttttttcaaa atcaaaccca catacaacac actttgtacc
    19501 atgatatttt attgcctcta ttctattctt aggattacgc tcatattgtt ttccataaac
    19561 atatttaact tttccttctt ttctaatttt agtttctaat tctatattaa tagtatcaat
    19621 gtctaaagga ttatcttgac ttatttcctc taatacatca tcttctattt cagaagtaaa
    19681 aataaattct atatcaaaat tattactatc aaattttaca aatcttattt taggtaaatt
    19741 cgtattttct tctattttac cttctaagaa tttctcataa tatttaggga atatatttct
    19801 tagtaaatct ctaaacttag atttccaaat aattctactt cttggattct ctaatttatc
    19861 catctctata actgcactat aataattatc atcatatcta aaattcacat ttttcttgtc
    19921 attcttccct aaattattta tctcaaagaa atgtcttatt tctattggaa cccctgtacc
    19981 attatgtttt aatgtagatt tatcaattgt ttttaaagct attttctcat ctaataattc
    20041 ccaagaatca actattatag gatactcatt tatatttttc ctttctaaaa gtatattaat
    20101 aatttgcttg aaatgtatct catcttcttt aaggaattca atggctttat ctgtcgagta
    20161 tcctctaaaa atacctttat gatttttatt tattcctaaa tgatgtaata tacccattga
    20221 ttgataacct ttactttgtt ttgatgataa atctagtatc tcttcatcta tctttctatt
    20281 actcatatta tttttaaata aataattata aactactttg tttatttgct ctgatgtgta
    20341 tttattaaac tctctttttc tatcattagg ctctaactta ggtattatca tattctgctc
    20401 ctcaattgat tgttttagat tttagtaaaa ttattgtatc atatatttat tattaaaata
    20461 tattgtgtta tttatcaaaa tacgcaaatt atacaatgtc attgattgca taaaaattaa
    20521 agagggcatt attataccat ctttatgaaa tatattttat tttaaacaat ataattaaaa
    20581 tatactccac ttaaagtatt ctagtacaac ttattaataa tctcaattta ccatttaatt
    20641 attctattga aatacacaaa ttaattactt caacaactct attataaatt ttgctcctgc
    20701 tttaaatcct aatttataac taagttccct ttcaaattct attaatgaat taaacaaatc
    20761 atttaattca accaacacac acttcttatc atcttctaaa cttttttcca ataactcatt
    20821 aattttttca acctctttcc aaccttcaat ataatactca tctttttcta aaatatctct
    20881 atacatgtgt tcatttatcc aataatcaaa atatatttct ataatttcat catacatttt
    20941 agtacctcct tgactttcct caggcactta atatatactt aagttgctag gttagtgtac
    21001 atcaagtacc taatttataa aggtagttgt tatatttata tggcaactac ctttttctaa
    21061 taagtgttta aatctaaact aaatatattt taaatcttta ttacatctaa ttaaaattta
    21121 tatttatact tcaacacttc ttgaaacata atttatctat tacttaataa atcatgcttc
    21181 ttttttatat ctcttgtatc ttcttacatt cttcaaaaga aaacaaattt cctatatacc
    21241 aatatggtct aaatcctgac tcaccatccc tatttttagc aacaataatc tcacttaaat
    21301 tagcaccaaa acctttttct cttcgtctca gttctcttat atcctcaatt cccattgtct
    21361 tacaataatt aagagcttct ttttctgtag gtctatgaat atatataact gaattagaat
    21421 ccatatatat ctgctttgag cttctcatca ctctatcacc tttaggtctt tcatctttca
    21481 attcatcatt taattgagca aactgaataa ctggtatatt aaaatctaaa gtaatctttt
    21541 ttatttgcct tgataagtca gcaaatttag cttctcctac ttctttacct tcataatcca
    21601 gaatttgcaa ataatcaatt atgactaagc taggcttaat actctttatt tctctcttta
    21661 ttcctgatat tgtatttaca gagtcattta tataaagatt attttcctga tttattatac
    21721 ttaaagcttg tatatatttg actatttctt catcatctaa gtttctattt ttcattttta
    21781 caactgatat tccagttagt gaggctatat ttctaaatag aatctgtata tcgctcattt
    21841 ctccacttat aaaaagtact ttcttacctt gaaatattac atttctaagt atttgtaacg
    21901 ccaatgctgt tttacctctt ccacttttag ctgctattgt agttagttcc cctccatata
    21961 aacctcctat agtatcatct aagaacttta ttccagtctt aataccttca tttacttcat
    22021 catttatata ttcaaaggct ctctctgata tggctgttat acttgaatcc ttttttatta
    22081 atttctgatt agctttattt atatcatttt ctaactgata tataagagta ttatcatcta
    22141 tttctccaaa gctgaattta ttaaatttat caattatatt tctatttttg tatttttcaa
    22201 taactatact gacataactc atgaaatttg atactgttgg aactatggtt gacaaactag
    22261 tgatatagct tatacctcca atattattca aatgaccttt tctctttaat tcttctgtaa
    22321 gagttattat atctattact tcatttctct tatttatttc ttttatagca ttaaatatgt
    22381 atttatgttc ctcatgataa aacatgtctt catttaacat ttgctctaac tcttgtacta
    22441 ttgttgaatc taataaaata cttcctaata ttgattgctc tgcttctgta tttttaatta
    22501 agttgtttga attatttata atataacctc cttaccactt taattgtgag tcatctggat
    22561 tatatgttcc tgtctcttta tttatgacaa tttccttgct atgataattg ccctctagta
    22621 tcttggtaaa attacttgaa ctcattatcc aatcaaaatt tgctttccat cccgtattat
    22681 tatttccatt cagaaaactt gttttactaa ctatatttat aacctctaat acagtttctt
    22741 tatcatattc tcttattctt gaatttattc tcctctttct tgtgtctgtt agtttcttga
    22801 cctttgaaag ttcaatacaa gttgagttaa atttgcttat aatttcacta tatataatat
    22861 tattttcatt ctttatattc ttgattgttg ttgattgatt gttaatcgtc tgttgattag
    22921 gttgttgatt ttctatgttc tcctggtaaa gctcccagtt ttcaatagtt atgactatat
    22981 atttgtttgt tgtttcgtat gttagaaaac cagcattttt aaaaaacaac aaacaacttc
    23041 taaccatttg ataactaact cctttaccac atctttcagc tattttgcta taggaagaaa
    23101 taaattgtcc agattttatt tttatccttt tattatctat aaaaacatgg ttttctttat
    23161 gactagccat aagtaatata gttattaata ttacttttct ttctggttta atgtctttcc
    23221 aaattgattt atccaacaac tccctataaa gctttatcca ccctttattg ctcaattaca
    23281 aacacctact ttaaaactat aacttgatac catttgcatt ttcaataaac caatcatcca
    23341 atttattttt tattatgtat actcgattat ttatccatat acaagggaat cctttatttt
    23401 tagaatgaca taactgccta aattttgatt ctcctatagg ataaagtctc gatgcttcct
    23461 tacatgttat aagttcagct ttatttcttt tcgcattttc aataatttta tatccttgct
    23521 ttttttcttg tgcttcttct tcattcttta caataatgtt taaagtagct tgtaatgtct
    23581 ttaaaacctc taaattatta ttctctttca tattttaatc ctccttacta aaaaataatt
    23641 cttctactgt tgtatctaaa gctctagata gtttaatcat caaatcattg cttggattat
    23701 tagccttacc tgtttccaag tttgataaat aatatcttga tataccaact ctatctgcca
    23761 gttcttgttg ctttaaacct tttttaatcc taaatatttt taaattgatt cccatacttt
    23821 ttcctccttt tttctagttt acattcgtaa actttacatt ttaattataa tgtcgacata
    23881 tgcgtactgt caataagaat atattatttt tctgttctca tttgtgtact tttgggatat
    23941 actttatacg aggtgataat attgcaaatt ggaaaaaact taaaaaagat aagaaaacaa
    24001 aaagaactaa cacagattca acttgctgaa attagtggca tctcaagaaa tgctctaata
    24061 aattatgaaa atgataaaag aatacctagt atagatacct tatcaaaact cgctaaagct
    24121 ttgaaaattg aaaagactat cttttatttt gatattgaaa atgaagtaat gactaatgaa
    24181 gatgttttat actttttaca gtctttattc ccagaagaat actattcttt tgatgaattt
    24241 ttaaatgcta tttcaaatct tatggaagat gatgatatta gaattacctc aagtttactg
    24301 ttaggtgctt ggtctgataa agaccaatat tataaacttg gtgaaaaatt agaactaaat
    24361 gaaaatcaag tttataattg gatactttca gattgtatat ctaataccga attagatttt
    24421 tcattacatc ctgatgacat aaaagaaatt attaaaaatg atgttcttac aaaacaatca
    24481 attgatgaat tattagatat tggtctatca gaagaaaata tagaaacaat ctatgattac
    24541 tttagtgact caacagataa tggaagtaat attcgactaa aaaaattaat atctcaaaaa
    24601 gaaaatatta ctattaaaaa taatgattac tttaaattat tacaagaaaa taacgaatta
    24661 aaagaaaaaa atgaaaagct tgaaaataca cttaaagaaa taaaaaaact actaactaaa
    24721 atataaaata atatcaatac tatctaaagc aatgataaaa aactatgaaa ggagctacaa
    24781 tcatgaacaa atcatatata ccaaaataca catataaatt tcctactaat ctacctcatg
    24841 gagataaaaa atatacttta acatacatta gaagaatgat aagtgaattt gtatatgata
    24901 tgggtaacct agaaaacaat ccctttacat tcccagaagt acaaacatta cttgatggta
    24961 taaccgtagg tggacataaa ttaagtgacc aaaatcaaat actaaatata aaatcatcat
    25021 gggattatgt aataaaatta agtaacaaag aaaaattatc tttaaataaa gatacaattt
    25081 gctctataca taaaatagtt gcaaaagatg aagctctaat cgttggagat ttcagaaatg
    25141 gtaatatagg tatagctggt actacacaat atgagtgcat agaagccact ctattaaatg
    25201 atttatttgt aaatgacata aatatcataa ataaaataac taacccctta gaaaaggcta
    25261 taataataaa tttatggttg tcctattgtc aattcttcta tgatggcaac aaaagaactg
    25321 ctagacttac ctcaaaccta atacttataa gtaatgatat aggagttcta tcaataccag
    25381 ctaaacataa acaagaatac aacacattaa tgctaaactt ctatgaaaca ctagaaggtg
    25441 atgaagttat aaaattctta ttagagaaat gtattacatt ctttgatgga tataactata
    25501 aatcatataa ggagctgttt aaaaatggca actaaaagag ctaatggaga aggctctatc
    25561 ggaaaatata aagatggttg gcgttcccgt ataatgattg gttacaatga agatgggaaa
    25621 ccaataagaa aagaatttta tggtaagact caaaaagaag ttaaagataa attagatgct
    25681 tataaaaaac aatattattt aagttctgat atttcagatg ataaaataac tttagaacat
    25741 tggttttaca attggttgtt tgaatataga ataaaagatt taaaacctaa gtcatttgaa
    25801 agatatgaaa gcatctatcg taattatatt aaagatactg atttaggcaa tgttaagtta
    25861 aaggacttaa gagtatctca catacaaaaa tattacaata gattgcttga ttccaataaa
    25921 agtataccta ctataaggca aataaacact aaactgaaaa cctgcttgtc agaagctgaa
    25981 aaacaaggtc ttatacaacg taattattgt actatggtaa atataccagt tgagaaaaaa
    26041 gaaaataagc ttgaaatatt gactcttgaa caacaaaaga gttttataaa agctatagag
    26101 gggcataaat tagaagtatt gtttttggtt gctcttggta ctggacttag attaggagaa
    26161 ttattaggac ttaaatggtt cgatattgat tttaagaatt ctaatttaac agttaaaagg
    26221 acgcttcaaa gaacatattt tatagataaa aatggagata gagagcttaa agtattagag
    26281 caagagccaa agacatcaaa ttcttacaga actgtaccta ttccaaagga tgtcttaaat
    26341 aaattgaaag aacataaagt aaatcaaaat acagatatat taaaggctgg agaattatat
    26401 agaaatgata attatgtatt ttgcaatgaa ttaggtgtcc ctgtagatga taaaagacct
    26461 ttaaggaatc taaaatctat attaaacagt ttagatatag agcctataaa gtttcatggt
    26521 cttaggaaaa cttatgcaac tagacttttt gagaatgatg tccctcctaa aactgtacaa
    26581 gttttaatgg gtcattatga tatatcaata actttaaata tatatactca agttatggaa
    26641 gataagaagg ttgaagctgt agaaaagttg gataaaatat tttcattgta aaaatcaaat
    26701 atataaaaac gccatgatta catataatta attataaaat caaaataagg atacaaatta
    26761 attttatatt atcttgtatc cttattctta tctgattttt taactattta tcttgaatag
    26821 cctttatgtc ttgagctaag tatatatcaa tccttacagt gcatatctga tacaccttca
    26881 cttataagtt tatatgtttc agaagtatat aaataatcca tactttcttc caagctcttt
    26941 cctgtttgtt tagaaaatta taatatgatt cttttatata gtgattgtaa caatgtctta
    27001 ttagcttcca taaaaactat acctcctctt tcaactattt atttatccta ttttgactaa
    27061 ctatatattt attagttatt taattagcca taataattcg gctataagat atttataatc
    27121 taatctcaat tatataaatc tttttactaa tatttaataa tcaaaatatt gcacttaatt
    27181 aactatctta taaatattga taataaaatg tgtcttctta aatatactta aataaaattt
    27241 ctataattat atcaaggtat taatattcac tctctattgt ctctcctaca agtctatttt
    27301 ctccatttag ggtatatact cctgatatag ttgattgtat gtattttcta tccatatatg
    27361 gttctaaaac tttttttatg ctagcattgt ccaaaagaca ctatatcctt gttcattaag
    27421 tagttcacta ctttcaaaac aaacaacttc accataatca ttatatagaa tcgaatcttc
    27481 cttaccatat catttatgct tggtaaatca ctaaaatttt ataactactc atattagtta
    27541 ttggagaata taattatact tatgtctcat actcaaatat tttacataat atataagtct
    27601 aaattaagtt gcaatataaa agcacctctt aatataggcg ctataatgtt ataaattttt
    27661 aattatatat tccaatgaac ctttatcaac catatccaaa ttaaatatat aatccatctt
    27721 atcaaatgca tctcttaatg gaatacttga ttcaagccat cctaacccat ctgtaatcca
    27781 acaaaatgta aaacctccat cttttatata cttatctaac ttagtaaatt catttgcaac
    27841 agattttaac ttagaacctc caccattata aaaattaact tctactaaaa caatttttcc
    27901 attattattt atagcaaagt catactttct tttactctta tctgttggaa cttctataga
    27961 ccatctctca cttatttttt tcactgtagc ttgtgttaag taactccaat tattcttttc
    28021 acacatattc tttacatatc tttctacaag agtttccata ctacttccag ttctattttt
    28081 tctagcattc gtatcaagtc caacctctac tccaaacaca taatctacta aattttttat
    28141 atttttttgt tttaataagt ctaaaaactt tattttttta agaaattctg tagctaattc
    28201 gctttcttca tcacttatac tctttctctt cttaaaatta aacagtttat atttaaacat
    28261 atcctcatct gatggatcta gtattttgaa ttcattatct ctactcgcta ttactattgg
    28321 tatcacacta agaacttctg gatactcttt taataaattt ttggtttcgt tttctatatc
    28381 ttcttttcct ataagatagt ttaagatatt taactgaacc tctatctttc tagtattttc
    28441 caaagcttta ttccagttaa cataataatc ccagctagct atagtacctt ttaaattttc
    28501 aagtaattcc ttaaatattt catccttact cttattttta tgaatattct taaccaattc
    28561 catttgattc cccacctctt cataattagt aattagcaat tcagttataa aacctcttcc
    28621 attcccctta gaatttatga atcttttagc atgtactctt tttatattaa aatctgaata
    28681 caaatcataa aagaaagtat cttcttcatc taaaactttt ggatcagaat tacttaacat
    28741 caattttgta tctattttat ctaaaacttt ataaaattta cttaactcaa tttgactatc
    28801 atcattaaaa ttacctttgt tataggaatt aaatcctcct atagttactg gtctatatgg
    28861 tggatctaga tacacaaaac aatttttatc tattatattt tctagattta aataattctt
    28921 atttaatatt tttactttta gctctcctcg agaatctctt ttatttaaaa gcatggataa
    28981 tttttcaagt tcatttttat caaaaaaact aggattttta tatcttccaa aaggtacatt
    29041 aaattcacct cgagaatttt ctctatataa tccattaaaa catgttttat ttaaaaatat
    29101 aaaatatgag gcttgcttta tacaatcttc attttctata tttttttcat ttgtaagtat
    29161 attaaatcta tctctaatat catagtacat cttttctcta tctgaagaac cttcttcgta
    29221 agatatgtat tgttctttta aattagttag ttctgttatt aactcttcca aatcatctct
    29281 gactttaata tataaattaa ttaacttact atttatatca ttaattacat acttgttgaa
    29341 aatgtcctct tctaacaaat atagaaatag tgcacctgca cctacaaaag gttctatata
    29401 agtcatggaa ctaaaatcac aacctgtagg catttgtttt cttagaatat caagtagctg
    29461 tgttttacct ccagcccatt ttaaaattgg tttcatgcaa cacctctctc agttatattg
    29521 ttatataatt atataaaata agattctaaa atacaagtac tcatcagaac actatattta
    29581 tatcaaatct ttccaaaatc taagtattat tttatcctaa cacttttcaa aacctctaaa
    29641 taatatacaa tattctctaa tatttactta ctaaaaattt aaatctatct ttaagtataa
    29701 cctaaaacag attataagtt acgtagtttt taatataaaa tatacttcta caagtaaagc
    29761 tcaaatatca attacttatt tcataattaa tgactatgta cccccaaaaa aacctctttt
    29821 tctgtggttg gggtacggtt ggtggatttc atcaccaaaa caaaaaatac tagtcaccaa
    29881 agactagtat ttctaagtat ttcatcattc ttttttcaaa attggtggag acgaggggag
    29941 tcgaacccct gtccgcaaat cttttccttg ctgcatctac atgcttagcc aacttcacta
    30001 atatctcacc tcacatagcg ccaagtggca ggctctatgc ttagccagtc ccaattaatc
    30061 tacttcaggt cgagacaccc ctaaagtagt tccctgcata atgacgtcca gtcttagtcg
    30121 gcaggaggac taagagggac gagcagcaac taggctgcta tcgcgaaatt atcgtctgcg
    30181 tttattttaa agttccccag tttttacgtg acccagagta acacgacatg cagcctcaag
    30241 ttccaaaccc acgtcgaaac ctttacgccc ccttgagtag tttatagtcc agtagacttt
    30301 tcgcaaactt tatcaccatt tttattaaat ttttaatgta tatataatat aatactttta
    30361 agaataaaaa tcaatgttat gttctagata ataaagaatt ttattctaaa aaatacagtt
    30421 ttatctagtt taatacttac ctgtcatttc tctttctata cgtctttgtg catctttctt
    30481 agccatatct tgacgtttat catatagttt tttacctttt gctaaggcaa tctctacttt
    30541 aactttacca ttttttagat acaaactaag tggtataagt gaatacccct ttatagtagt
    30601 tgctccaata agttttctta tttcatgctt atgaagtaat aattttctaa ctcttaaaga
    30661 atcaacattg aatatatttc cttgctcata tggacttata tgaacttgtt ttaaaaatac
    30721 ttcgctatta tctacagatg caaatccatc acttaggtta actttgcctt gtcttattga
    30781 ttttacttct gttcctttta attcaattcc acattcataa gtttcttcta taaaatattc
    30841 atgtcttgct cttttgtttg ttgctaatat ttttttacca gccatataaa tctcaccttc
    30901 tttgctagaa atttcttcca taaatattag aaatttacta aatatttaat gttgccaaat
    30961 tatttgacta acttgtatga taacatattt aggcatttta gtcaatattt cactgatata
    31021 ttaagttagt taggctatat ataattaagt aatataaatg tatgaaaata gctaaaaaac
    31081 tacttcgaat atacttaata ctaaactaac tctaaccagt aatactaatt tataaatttc
    31141 aaaacaaaaa tattacatat tataaatctt ctatcttcta tcttctatct tctatcttct
    31201 atcttctaaa taattttaac atgcccataa aatataatac ccacaaatga taaataatac
    31261 tcaaaatatc tattgaaata actatacatc cttaaataac ttaacttaaa tctaaacatt
    31321 tcaaaaactt cttaaaaata tgtaaaaatt tgatgataaa gtagcgattt tttcaaaaaa
    31381 aatgtaatat agagttatat cttataatat tttagagata aattttatca tagtggggag
    31441 aaatttatat ggatttaagc aaaaaagtaa aaattttaga aaatgcatca gatggcaaaa
    31501 gcgaatttga gatattagaa tttgaaaaac ttgagggagc ttctagccca ttaatggcaa
    31561 tgggattata ttttgctaga gaagctggtt tgaaagctag acaaattaag ataaaattaa
    31621 ataattcatc aataaaaaca gaagctggtg cattatacta ttataaagga aatatatctt
    31681 ctgaaactaa gattggtggt gttggaggtc tttttaaaaa aggaatatca ggagcactta
    31741 ctaatgaatc aatgatgaaa cctgtataca aaggtactgg agaagtttat ttggaacctt
    31801 cttttagaca ttatttattt atgagtcttg ataatgattc tataattgtt gataaaggat
    31861 tattctattg ttgttctgat acaatagaag taaaagcatt cacacaaaag aacttctcat
    31921 ctgctttact tggtggtgaa ggcttcttcc aaatagaatt aagtggtact ggtgtaatcg
    31981 ttctggaatg tgttgtacct caaagtgaaa ttgtagagta tgaattgaaa aatggtgaag
    32041 aattaaaggt agatggtaac tttgcaatag caagaacatc tggtgttaat ttttctgtta
    32101 caaaatcaga taaatcttta attggttctg ctttaaatgg tgaaggtttc ttaaatacat
    32161 ttactggtaa tggtacagtt tggctagctc caactcaagt aatgtatgaa aaactaagtt
    32221 ttggagcaat gcctactaat gataatatga ataataaagt ttctaagcaa aaatagttgt
    32281 tttataaata tttattaaat cattattgat tttataaagt cactataaat tttattagat
    32341 tattattata aaatcagagt gtggaaatgt ttatgtgtat tctttaaata a
//
LOCUS       DAEMHW010000026        32370 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEMHW010000026 DAEMHW010000000
VERSION     DAEMHW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.026"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      121..211
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            617..934
                     /gene="flgB"
                     /locus_tag="KPK67_001002"
     CDS             617..934
                     /gene="flgB"
                     /locus_tag="KPK67_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBG2457999.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            942..1346
                     /gene="flgC"
                     /locus_tag="KPK67_001003"
     CDS             942..1346
                     /gene="flgC"
                     /locus_tag="KPK67_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBG2458000.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            1356..1673
                     /gene="fliE"
                     /locus_tag="KPK67_001004"
     CDS             1356..1673
                     /gene="fliE"
                     /locus_tag="KPK67_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBG2458001.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1698..3248
                     /gene="fliF"
                     /locus_tag="KPK67_001005"
     CDS             1698..3248
                     /gene="fliF"
                     /locus_tag="KPK67_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBG2458002.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEQEDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            3261..4331
                     /gene="fliG"
                     /locus_tag="KPK67_001006"
     CDS             3261..4331
                     /gene="fliG"
                     /locus_tag="KPK67_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBG2458003.1"
                     /translation="MSMEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            4324..5055
                     /locus_tag="KPK67_001007"
     CDS             4324..5055
                     /locus_tag="KPK67_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBG2458004.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKINAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            5059..6378
                     /gene="fliI"
                     /locus_tag="KPK67_001008"
     CDS             5059..6378
                     /gene="fliI"
                     /locus_tag="KPK67_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBG2458005.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            6409..6855
                     /gene="fliJ"
                     /locus_tag="KPK67_001009"
     CDS             6409..6855
                     /gene="fliJ"
                     /locus_tag="KPK67_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBG2458006.1"
                     /translation="MELDKNFKFRLQKVLDLKVKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            6884..8089
                     /locus_tag="KPK67_001010"
     CDS             6884..8089
                     /locus_tag="KPK67_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBG2458007.1"
                     /translation="MKIQADSYNIIKDIGDRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KNRREVSKQNISSDKPENSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     DNYKKDKDINTILNNIEKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVISKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDKVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENKSKKNTNDKHNNVVIEDDIKENII
                     DDDRVDLLA"
     gene            8107..8715
                     /locus_tag="KPK67_001011"
     CDS             8107..8715
                     /locus_tag="KPK67_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBG2458008.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            8742..9725
                     /locus_tag="KPK67_001012"
     CDS             8742..9725
                     /locus_tag="KPK67_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBG2458009.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9738..9941
                     /locus_tag="KPK67_001013"
     CDS             9738..9941
                     /locus_tag="KPK67_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBG2458010.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9941..10759
                     /locus_tag="KPK67_001014"
     CDS             9941..10759
                     /locus_tag="KPK67_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBG2458011.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKKMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10749..11444
                     /locus_tag="KPK67_001015"
     CDS             10749..11444
                     /locus_tag="KPK67_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG2458012.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKEDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPKLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11456..11899
                     /locus_tag="KPK67_001016"
     CDS             11456..11899
                     /locus_tag="KPK67_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBG2458013.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11914..12288
                     /locus_tag="KPK67_001017"
     CDS             11914..12288
                     /locus_tag="KPK67_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliO/MopB family protein"
                     /protein_id="HBG2458014.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMNKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            12289..12954
                     /gene="fliP"
                     /locus_tag="KPK67_001018"
     CDS             12289..12954
                     /gene="fliP"
                     /locus_tag="KPK67_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBG2458015.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12967..13236
                     /gene="fliQ"
                     /locus_tag="KPK67_001019"
     CDS             12967..13236
                     /gene="fliQ"
                     /locus_tag="KPK67_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBG2458016.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            13248..15062
                     /locus_tag="KPK67_001020"
     CDS             13248..15062
                     /locus_tag="KPK67_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBG2458017.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            15071..17146
                     /gene="flhA"
                     /locus_tag="KPK67_001021"
     CDS             15071..17146
                     /gene="flhA"
                     /locus_tag="KPK67_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBG2458018.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            17121..18137
                     /gene="flhF"
                     /locus_tag="KPK67_001022"
     CDS             17121..18137
                     /gene="flhF"
                     /locus_tag="KPK67_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBG2458019.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFS
                     KKDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLDLN
                     QEIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            18134..19012
                     /locus_tag="KPK67_001023"
     CDS             18134..19012
                     /locus_tag="KPK67_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBG2458020.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENDNVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            19026..19727
                     /locus_tag="KPK67_001024"
     CDS             19026..19727
                     /locus_tag="KPK67_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBG2458021.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19743..20165
                     /locus_tag="KPK67_001025"
     CDS             19743..20165
                     /locus_tag="KPK67_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG2458022.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRSNNHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            20150..20335
                     /locus_tag="KPK67_001026"
     CDS             20150..20335
                     /locus_tag="KPK67_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG2458023.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            20376..21149
                     /gene="flgG"
                     /locus_tag="KPK67_001027"
     CDS             20376..21149
                     /gene="flgG"
                     /locus_tag="KPK67_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG2458024.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            21170..21931
                     /gene="flgG"
                     /locus_tag="KPK67_001028"
     CDS             21170..21931
                     /gene="flgG"
                     /locus_tag="KPK67_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG2458025.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRIYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFGAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21954..22835
                     /locus_tag="KPK67_001029"
     CDS             21954..22835
                     /locus_tag="KPK67_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG2458026.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFEKIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22838..23212
                     /locus_tag="KPK67_001030"
     CDS             22838..23212
                     /locus_tag="KPK67_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG2458027.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            23244..24647
                     /locus_tag="KPK67_001031"
     CDS             23244..24647
                     /locus_tag="KPK67_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2458028.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGIYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNILDMYNSANIQTKSNIKVDDYKNEFLHKSSNNHKIND
                     YNNVTKEKSLSKKQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNKTKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKESDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKSSKLTLRKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24763..26700
                     /gene="htpG"
                     /locus_tag="KPK67_001032"
     CDS             24763..26700
                     /gene="htpG"
                     /locus_tag="KPK67_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBG2458029.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDTRNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEVVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFHVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDDEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            27087..28217
                     /locus_tag="KPK67_001033"
     CDS             27087..28217
                     /locus_tag="KPK67_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG2458030.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKVSIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            28444..29466
                     /gene="splB"
                     /locus_tag="KPK67_001034"
     CDS             28444..29466
                     /gene="splB"
                     /locus_tag="KPK67_001034"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBG2458031.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKNIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLASAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKGFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29648..30559
                     /locus_tag="KPK67_001035"
     CDS             29648..30559
                     /locus_tag="KPK67_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2458032.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30574..30990
                     /locus_tag="KPK67_001036"
     CDS             30574..30990
                     /locus_tag="KPK67_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG2458033.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(31090..31482)
                     /locus_tag="KPK67_001037"
     CDS             complement(31090..31482)
                     /locus_tag="KPK67_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2458034.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31623..32018
                     /locus_tag="KPK67_001038"
     CDS             31623..32018
                     /locus_tag="KPK67_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG2458035.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT        13280 a         3416 c         5915 g         9759 t
ORIGIN      
        1 aaatataaat atattaaaaa tattataaaa tttaatatta gaaattaaat taaaaaatta
       61 tataaaaaaa tacttaaaaa aatatttaaa aaatttgtta tttattgtat acttaagtta
      121 aaactaaata ggcaaatcta gagaaatcta gtgacgcaaa gctataggga ctaaggttta
      181 tatacataaa ctatgtcagc cagttgccaa aaagagtcct aggtgtattg tatacctaag
      241 aaaagtctat aatgactgcc tttttggcag tcattttgtt atggaaaaat cactttattt
      301 cagtagaaaa tacatataat aacatatttt accaattata acataaaata aagaaaaatt
      361 atctcaaaaa gttactattt acaaaaatga cttctttatt tatttaaatt aatcgcattt
      421 ccattacatt aatacaataa tgtatcaatt tagtaagaaa actaatattt ttttaatgaa
      481 gtcccatgaa aatataaaaa aattttaata tgtatttaaa tatttcttcg aaaagttgct
      541 cttttttgta aatatagcaa cttcattttt tattaataaa caaaatagat aaaatatatt
      601 ttgaggaggg gtgagtgtga atacatatga cattataaaa atgggattag atgcaactaa
      661 tctaagaagt cagacaatag caaataatat agcaaatata aatactgaag gatataaaag
      721 acattatata acttttgaag aaacactaaa tgatgctatg cctaaaatag aagaaaaaac
      781 agacaattct aaaggtttaa gagtagatgg taataatgta gacatagaag aagaaaaagt
      841 aaatcaagca atggcaacat tacaatataa tgctttgata agttttgcaa atggaaaaat
      901 agcaatgacc aaatcaataa taagtgggag gtaattagaa gatgagtgtg ttcagtggaa
      961 tgagaatatc tgctagtgga ttaacagcag aaagtttgaa aatggatatc atatcatcca
     1021 atgtagctaa tataaatgct acaagaggtc caaatggaga agcatatagg agaaaagttg
     1081 tttcatttga agaaaactat gataataaat taggtatgct aggagtaaaa gttacatctg
     1141 ttgaagatga taaatcacct cttaaagaag tatatgaccc atctcatcca gatgcaaagc
     1201 caaatggata tgtagaatat ccaaatgtaa atatattggt agaaatgaca gatttaataa
     1261 cagcttcacg agcatatgat tctaacgtag atacattgaa tgctcagaag tctatgatat
     1321 caaaagcttt ggaaatagga aagtagggat aaattatgcc aattacacaa attaacaatt
     1381 taatgcaagg tgcaaagtta gataatttga ttggagaagc aaagccatta actcttaata
     1441 ataaagaaga aaaaaagaat tttgaagatg taataataga tactatagat aaagttaatt
     1501 ttcaacaagt aacagcagat gatatgaaag aaaaatttat aaaaggcgaa gatgtttcta
     1561 tgcatgaagt tatgttaata ggtcaagaag cacaactatc tatgcaatat ttaatagaag
     1621 ttagaaataa gattcatgat gcatatcaag aaatgaataa ggtgcaaatt tagccttttg
     1681 aacaggagta ataatcaatg ataaccagtt ttaaaaataa gattaaggag caagttgcta
     1741 aatctaggac aaagataagt tctttaaaaa agggaactaa aatagcatta ggtatagggg
     1801 ttattgctat atgtttagcg ctagtattta cagcaatatc tgcgagcaaa aataagtatg
     1861 cagtcttatt ttcagaacta gatgctcaag atgctgcaga tatatcagca gagctagagt
     1921 ctaaaaagat agatactaag gtaaaaggaa gtacgattta tgtacctaga acagaagttg
     1981 ataagttaag gctggagtta gcgccaagtc ttacaggtgg ttctaaagga tttgcactaa
     2041 tggatgatag ttcatcaata gggcttactg atgaagaatt tcagattaag aaacaaagaa
     2101 tgatacaagg tgaaatagaa aaaacaataa agagttttcc acaagtggaa gacactagag
     2161 tacatataac atttggagag tcatcagtat ttgagaagga ggctgttcca gggagtgcag
     2221 cagtttattt gatgctaaaa ccagggacga ctttagaaaa aaaccaaata aagtctgtta
     2281 tggcacttat atcaggaagc gttacaaata taccagagaa aaatgttcag gttattgata
     2341 acaaaatgaa tttattatct gaaggtattt tggaggatga aaatgttgat gaaaataatc
     2401 aaccaataaa tagtggcatt agtgttgata aaaataaggc atctgaacaa gcattgaata
     2461 aacaactaga aaagtctatt ttagaaatgc tagaaccgat atttggtaag ggaaaggtga
     2521 aggctacagt aaatgctgat ttaagttttg atacagctga aaaaactgag ataaaaattg
     2581 accctgataa agttgcaata aaggagacaa ggtcgaaaaa ttcatctaaa tctgctgcaa
     2641 ataatgttca aggtgtagat gctaacatga ataaccaagg aaataacaat ggtactaata
     2701 cagaagatag tttagatgag agttttgagt atgaaacagg taagattgaa actcacacta
     2761 tagttgcgcc tggagaactt aatagattga cagcttctgt tgcaatagat ggaaaaatag
     2821 ctaaaggtgt tcaagctgat gtagaggata ttgttaataa tgctataggt atggatggag
     2881 cgaggggaga tactcttagc gttgtttcaa tggtatttga cccagaaggt aagaaagagg
     2941 ctaaacaaga gctggaagag attaaaaaag aaggatttaa aaagaacata ataaaagcag
     3001 ttattggagt agtagcagta atagcagttg gagctattgg atacttaata tataagaaaa
     3061 gaagtgaaca agaagatatg gattatgaag atgaagatta tatttctaaa tctgattctg
     3121 ttgataggaa agataatcct ctattaggag atgacccaga actgacatta gaggaagctg
     3181 ttaaaatgta tgcagaagaa aagccagacc aagtaacaga gataataaag acttggttaa
     3241 atgagtaagg gggatagaaa gtgtccatgg aaattacaaa tgttaatgcg tttgacttaa
     3301 gtgatataaa tgaaagtgat aagaatgaga aggtagaagg tgagggttta aaaaaagctg
     3361 ctgtattatt aatgacatta ggcccaaatg tgtctagtga tataataaaa agcttgcctg
     3421 ataaacaagt tcaaaaaata ggcttggaaa ttgcaaatat ttcttcaata gcttcaaaag
     3481 aaagaagaga aatcttaaaa gagtttgtag aaataaatag ggcaaaagat tatgttatag
     3541 aaggtgggct tgaatatgca agagagttat taagtaatgc tttaggaaat gttaaagcaa
     3601 acaaattact tgaaggtata tcaataaata caagtacaaa gccttttaaa catattataa
     3661 aactagatgt aaagaagata ttagaaatac ttaggaatga aagtgcacaa actatagccg
     3721 ttttacttgc acatatggaa ccagaaaatg gagcagaagt tttatcatac ttagattttg
     3781 atttacaaaa tgaagtagct atgagaataa gctcaatggg ttcaatatct ccagaagtaa
     3841 taaaggtaat tgaaagttca ttagaagata aattacattc aataagtggt gatgatttta
     3901 atgattatgg aggggtgtct accttagttc aaatattgat taattctgat agaaaaacag
     3961 aaaagaatat tttgggaact attgaagaac aaaacccgga tttagcagct caaattaaat
     4021 ctcatatatt tgtctttgaa gatattgtca agttagatga tataagcata caaaaagtgt
     4081 taaaagaagt gactatgaaa gatttagtat atgccttaaa aggtgtagat gatgagattt
     4141 cagatgctat atatagaaat caatcttcta gagcagcaga tgcacttaaa gaagaaatgg
     4201 aaatggttgg tgttataaag gttacacaag ttgaggcttc tcaacaaaag atagctaata
     4261 tcgtaagaaa acttgaaaga gaaggtgcca taactattgt aaggaatttt ggtggtgact
     4321 ttgttgaata ataagattat taaaagtaaa aaagcattat ataaaggagt gctagattta
     4381 accataatag ataaagttag agtgggagaa tcaaatgtaa ataaagaaaa tgattgtaga
     4441 tttagggatg aggtaataaa agaagctata gaatctgctg agataaaaaa gaatgagata
     4501 ttagaagaag caaaaataaa tgcacaaaat atagaaaagc aagcatatga aaaagggtat
     4561 agtcaaggtc aaaaaaatgg atatgaggat ggatataaag agtcatatga tgaatatacc
     4621 caaaaagcta aagatgaagc agattttata aaaaatcagg cagacttaat actattacaa
     4681 gctaatgaga agatgactga gtacataaaa gaaaaaaata aagagattgt aagacttgct
     4741 attaatatgg ccagttctgt tttaaaaaag catttcgaag atgaagaatc tatgagtgat
     4801 ttgttaaagc aaattattca agattatgaa ggaagtagtt catttatagt aagatgtaat
     4861 tctttctata aagataatat agagaaagag tttgaattat tcaaagagga aacatcacta
     4921 aaatcaagtg tatttgttat tgcagatgat agtatagata aaggcaatgc tattatccaa
     4981 aaggaaaatg gaagaataat agttggtatt gattgtgcta tggaaaaact aaaagaagaa
     5041 ttgcttggtg aataaagtat gtataaaact gatatatcta tagattttga caaaataaat
     5101 aagaaaatag aaaatttaga actcactata tcagaaggta gagtaaagaa gataatagga
     5161 cttactgtag aagttgaagg aataaaagct tttgttgggg agttatgcgt tatatacaac
     5221 caagttaata aaccagtgaa ttgtgaagta gtaggtttta aagataaaga agtcatatta
     5281 atggcgttgg gagaattaac attgatagca cctggttgta aagttatttc taaggggata
     5341 cctttaagtg ttatgtgtag tgataattta ctaggtaaag tacttgatgg tcttggaaat
     5401 cctatagaca actcagatgc tgttttagga gatagataca acctgaacaa tgaacctcct
     5461 gacccaatga agagaaaaaa gatacgaaat ataatggaaa caggtgtaag agcaatagat
     5521 gcatttacta cttgtggtga aggacagaga atcggcatat ttgcaggaag tggagtaggt
     5581 aagagtacga ctctaggtat gattgctagg aatgcaaaag cagatgtaaa tgtaattgct
     5641 cttattggag aaagaggaag agaagtacta gattttattg ataaagattt aggtgaagag
     5701 ggaatgaaaa aatctgttgt tgtgtgtgca acatcagata aagccccttt agttaggtta
     5761 aaaggtgctc ttactgctac tgcaatagca gaatatttta gagaccaagg caaaaaagta
     5821 atacttatga tggattcagt aactagattt gctatggcac aaagagaagt tggtgtagca
     5881 ataggagagc caccagctca aaaaggatat acaccatctg tatttgcaat gttgccaaaa
     5941 cttatggaga gaacagggac atcagataaa ggttctataa ctgcatttta tacagtatta
     6001 gttgatggag atgactttaa tgagccaata gcagatacaa caagagggat acttgatggt
     6061 catatagttt tatctagaga cttggcaaat aagaatcact atccttctat agatgtatta
     6121 aacagtttaa gtaggcttat gaatgagatt gcttcaaaag aagacataaa aatagcgtct
     6181 tttgcaagag acatgcttgc tgagtataga gaagcagaag atttgataaa tataggtgca
     6241 tatgcttcag gtacaaataa aaaaatagat gaagcaatct attatcatga gcatattatt
     6301 aattttttaa aacaagggat aaatgaaaag agttctttta atgaaactat aagtagtttg
     6361 agaagagttt ttgaataaac aattaaaaaa atatttgaat aggttgtgat ggaattggat
     6421 aaaaacttta agtttagatt acagaaagtt ttagacttaa aagtgaaaga tgaagaagag
     6481 ataaaaatgg aatttgctaa gattcaacaa aaaaaaatag atatagagac taatttagaa
     6541 aacttagaaa gtaattatag caaatattct ataagtaaaa ataacgatag tattcaaaat
     6601 caaaaaataa ctattaacta tttactagct ttaaacaatt ctataatgga tttatcagaa
     6661 gagttggata agtcaacaaa tgaacttgaa aaagctagaa agcagcttat aagcaaacag
     6721 atagagagaa aatcattaga aaaattaaaa gaaaaaaagt atggacagta ttataaagaa
     6781 gaaaatttaa aagaacaaaa cactaatgat gagtttgcaa gtatgagtta tttaaggaat
     6841 agacaagttc tataagaata gtaggaagtg atgatattat tgtatgaaga tacaagcaga
     6901 ttcttataat attattaaag atattgggga tagaaaaagt tcatctaaga ataaaaatat
     6961 aaaaaataat aaatttgaag ataatctata taaggcaaca gaccataaaa aggttaaaaa
     7021 tagaagggaa gtaagtaagc aaaatattag ctcagataaa ccggaaaata gtgataatga
     7081 gtgtcattta gatgaagata aaagtaatca cttagaagat gaaaatacat atgaaaaaga
     7141 ttgtagtaag aataatgaag ctttgcagca attactgaat cttttaaagg ataattataa
     7201 aaaagataaa gatataaaca ctatattgaa taatatagaa aaactaagta tatcagatga
     7261 attaaaacaa gaacttaagc ttaataaaaa tatattaaat aagattgcaa atcaaaattc
     7321 agagctaaat aaagctaata taaataactc taatgatata gaaaatttag acatagttaa
     7381 attaagagaa cttttggaag ataatgctaa taaagatatt gaagtaatta gtaaaactac
     7441 agaaaatgat actaaaaata tagatttgta tagttttgat agcaatagaa tggataatct
     7501 taactcaaat aaacagaaag atgatagctc tgatattttg gaaaaactag caggggtaaa
     7561 tggaaataag agtgggaatt ttggacaagt aataaataaa aatgttgata tgaataaaat
     7621 taaaaatgat aaagtcaatt taaaacagat agaatcaaat gtaatggatg actctataaa
     7681 agctattaag catatgaaaa caaatgatat acaagaattg acaattaaat taagaccaaa
     7741 agaattagga gatatgaata tacaattgtt aaaagatggt gaaagtatga gagctgttgt
     7801 gactgttttc aataaagatg tctttgactc tataaataaa aacatagcag atttaaagca
     7861 acatcttgaa cttactaatg taaatataaa agatgtatca gtgcaaatgc acagcgataa
     7921 tacaaataca tctgatacct ttgataaagc atttgaacaa cagaatagac aaaataacca
     7981 agaaaacaag agtaagaaga atacaaatga taagcataat aatgtagtca tagaagatga
     8041 cattaaagaa aatattattg atgatgatag agtagattta ctagcttagt aagggggaag
     8101 tattgcatgt acggaatagc aacggctttg gcaggaactc agggaaacta taggtcaaat
     8161 aacttaagcc aacttgaaag aaatattaat gatattgcca ataaacaagt tagaagcaaa
     8221 ggaaatcaga cagtaacaga caatgggact ccaattatag gacaaggtga tgatgctgat
     8281 aaagacttgt ttatgaagat acttgtggca cagatgggta atcaagaccc aatgaatccg
     8341 caagacccaa cagaatatgt gtcacaatta gcacagtttg caactttaga aaagatgtca
     8401 gctatgaatg agaagctaga tgtactttta gccttaagta attctactct aataaactct
     8461 gctttgagta tggcaacatc attaattggt aaagaagtag aagtatctgg ggaagagaaa
     8521 gaagatgaaa acattgtagg agtagtaaag tccacatata taaaagatgg ggaagtacac
     8581 ttagaattag aagtagatgg aagtgacaaa actgtaggtg ttaaatatga aaaattactt
     8641 aaaataggag aggtagatga aaattcagga gaagaaaatg actcttcaga acacgaatct
     8701 ccagaagaag cataatatta aatgagaaaa gaggtaaatt catgataaaa gcaatgtatt
     8761 caggtgtaag tggaatgaaa gcaaatcaaa caaaattaga tgtaataggt aacaatgtag
     8821 ccaacgctgg aactacttct tttaagaaaa gttcagctag aataacagat tcattttatc
     8881 aaactgtact atatgcaagt gctccaacag ctgcattagg aggaacaaac ttaggacaag
     8941 taggtgtagg tagtaagata tcaagtataa ataaagatat ggtacaagga aatgtacaac
     9001 caactaatag accatctgat ttaatgatag atggagatgg atatcttcca gtagtaagac
     9061 aaggaacagt tatgtatacg agagatggtt cttttaattt agatacagga tttaaagcag
     9121 ctgatggtgc tttgaagaat attaaaggtg gaagacttgt aaatggtgat ggatatatgt
     9181 tacaaggtat gatttatgat ggggaatata aagctgatac tggagaattt gttaaaaagg
     9241 gtgatgctaa aggaaaagag cctattgtaa taccacttca aagtataagt cctaaggatg
     9301 gaacagttca aaatgtacta gagtataata tatcaaagga tggaacagta gaatttttat
     9361 tgagtgatgg tcaaagaact caatttgtct atgatatacc tgatggtgct aaggtaggag
     9421 atacacctgc tggaatagat aaattaaaag gtacaataca aaaagtgcaa acatatgcat
     9481 tccaaaatcc agcaggacta gatgcagaag gaggaaactt gtttaaacca tcagcaaact
     9541 caggagaccc acaaatagca ggagctacag gaacaataat acaaggggca atagaagctt
     9601 ccaatgtaga tatagcagaa gaatttacag aaatgataat agcaagtagg tctttccaag
     9661 caaacagtaa aataatatcg acatcagacg agatacttca agaaataata aatcttaaga
     9721 gatagtaggt acatattatg ataagagtaa cggatttaaa cggaagaaaa tacatattaa
     9781 attcagacct tatagtgagg attgattctg taccagaaag taggattctc cttacaacag
     9841 gagaaaaaaa ccttgtaaaa gagtcggtag atgagatagt agataaagta ataaagtata
     9901 aaagaaaaat aaatgtaggt tatattagga gcgaaaaata atgaaaaaaa cagattcact
     9961 gacatttatt ggactaatag tatctacagt attagtttta gttggtgccg caaaaggaag
    10021 tagttcaggg ttaaagaatt tttttgatgt ttcatcaata ttaatcacag tgcttggttc
    10081 atttggagct ctaatgatta catttactat agatgatata aaacttataa aaaatgcatt
    10141 acaatactca tttaagacaa tgagtgtatc aaagttagat ttactagaac aatttaaaac
    10201 actttctaaa aaagctagaa aagaaggtct tttatcaata gagagtgatg taatgggaat
    10261 agaagatcca tttatgaaga aagggttaga gctgtgtatt gatggattgg aaatcaatga
    10321 gataaggtcc atcttagaac tagagataga ttctgttgaa gatacaaata acagatactc
    10381 taaactattt aaaacttggg gaacctttgc acctgctttt gggatgttgg gaacactaat
    10441 aggtttaata caaatgttag ctgaccttac atctccagat ttaatagcat ctggcatggg
    10501 aaaggcatta attacaacgt tttatggttc tttacttgcc aatatcgctt tgaacccaat
    10561 tgcatataat atagatgaga agactgaaaa agaaatttat gtaaagaaaa tgatgttgga
    10621 aggtataata agcatacaat ctggggagag cagtatagta gttgaggaac gtttagccac
    10681 atacctttca aacaatgaga agttagaaat tatgaaatcc aataaaaata cagaaagagc
    10741 aatgagcaat ggcgcgtaaa aaaaataaga aaaaggatga gataaatcca gatgcatggt
    10801 tggctactta tgcagatacg ataacactga ttcttacatt ttttgtgtta ttgtatgcta
    10861 catcagttgt agatatggaa aaatttaaaa atatagcaga tgcgttaaaa ggtcagtttt
    10921 ctgggacaag tatgtttagt acaggttcaa gtacaagtga gaaatctcca atagagagtg
    10981 ttttagtgga taaaaatgat aagtcaaatt tagaacagga tttaaaagat aaagttaata
    11041 attctaactt acaaaatagt gtaacagtca aagatgatag tagagggatt ttactagaac
    11101 tagatgattc aattttattt gactctggga tttctgaatt aaaagaagat agtaaagcca
    11161 ctcttaataa agtatacgat atgataaaga tgatgaaaaa taagatagtt atagaaggcc
    11221 atactgataa tgtacctgta aaatcaagta cacatgaatc caattgggat ttatcttcta
    11281 gtagagcagt aagtgttgta agatactttg tagaagaaaa gggcatggac cctaaactat
    11341 tttcggcaac aggatgtgga gagtaccaac ctttggtatc aaatgaaaca gcaaagggaa
    11401 gagctgagaa tagaagagtt aatatactta taactattga ctaggaggaa gaggtttgtt
    11461 caaaaataaa aaaattatta taattatttt gatactgatt attttctcag caggagcagg
    11521 tttttttata ttaggagaag gtggaaataa acaaattgac aagattgcag gtttgtttat
    11581 tccaacaggg aaaggcaaag atgaagtaaa gaccgaaaat cttgacttag aagagatggt
    11641 attgaagcta gctgatgaag gtgtaagata tttaaaagta tcaataactg tatcttatga
    11701 ttcttcatat aaagatatag aaaaaaatac gcctttaatt agggataata ttataaattg
    11761 ttttatggca aaaagggcag atgattttac tgctgataat ttaaagaaca taaaagaaga
    11821 aattaaaaat acattaaata tacaactagg tgaaaatgta attcaaaata tatatttcac
    11881 aaatatagta gtgcaataaa aggatggtaa gacatgatac tttatttact aaaaatatta
    11941 atatgtatcc caataatttt aatattgata gtcttatctt taaaaattag ccaaggaact
    12001 ttattaaaaa aatataatga taaatatata aaaatactag atattgtaaa tataagtaaa
    12061 aacaatagca taataatttt aaaaataggt gaagaaggat gcattatggc atcttcttcc
    12121 tctagcatga ataaaataaa agatttgacc aaagaagaaa tattaagaat agaaaacgac
    12181 attaaagaaa gaaattttaa aattcaagat tttaaaatgg aaagaattaa aaagaaatat
    12241 gatttcagta gatatattgg aaaatttaca aaaaaggaag ataagtaaat ggaactatta
    12301 tcaaaattac ctttggctga tgtaccatat acaagttcaa tgcaattgtt tttatttcta
    12361 actgcattaa tgtttttacc atttgtaatg ttcatgatga caagctttgt cagaatagtt
    12421 ataagtttat catttttgaa atcagcatta ggagcacagc aagcaattcc aagccaaatt
    12481 ttggtagggc ttgcaatagt attgacaata tttataatga gaccagtatt aaatgagata
    12541 aacgaaaagg cattacaacc atatatgaaa gaagaagtta ctatggaaga agctatgaag
    12601 gaagcagaag gacctattaa agaattttta ttaaagcaaa ccagacagac agatttagat
    12661 ttatttgtag agcaggctgg tttaaaagaa aagaaattaa ctcgtgaaaa tataccacta
    12721 tctgttgtag ttccagcatt tgctataagt gaattaaaaa ctgcttttca aataggtttt
    12781 ttaatatata taccattttt aataatagac cttgttgtgg caagtgtttt gatgtctatg
    12841 ggtatgttta tgttacctcc agtcatgata tcattaccat ttaagttatt gttatttgta
    12901 atggtggatg gatggaactt gatagtaaaa acattgatat tgggatttgg ataggagaaa
    12961 aatagtatga gtgaatttat gataatgagt gttcttaaaa atggcatgtt tacaggagct
    13021 atgattgcag ctccagtact cattgtttct cttgtagttg ggcttttaat aagtgtgttt
    13081 caagctacaa ctcaaatcca ggagcaaact ttgacttttg taccaaaact aatagctatt
    13141 cttttagtag tagctattgg tggaagctgg atgttacata ctcttattag ctttacttct
    13201 gagatattca atatgatatc tcttataact aaataagtag gatacatatg atttttgtat
    13261 ttattagaat agtagcattc tttggaacac taagaatact atttccagct ggtacacctg
    13321 ctttatttaa gtcactcttt gcaatcatta tatcaatatt gatttcaagt actatgcaaa
    13381 ttgaatatgc aacaaatata gacaacattg tattgtttac cttatatggt ataaatgaga
    13441 ccataactgg gattatgctt ggatatataa ccaacctatg tttttatagt atcagaatgg
    13501 cgggttctat gatggaccaa cagatgggat tatcaatgat taatatgttt gacccaaatt
    13561 caatgactca gacaacttta attgataatc ttatgaactg gacagcactt atgatatttt
    13621 ttagcatgga tggtcatcat gttttaatta gaggtataag gtatagtttt gagttaattc
    13681 caataggaaa accatttgta gataataaca tagattatat aataaacata tttgtgcagt
    13741 gctttttaac tggattcaaa atagcaatac ctattgtact ttgtctgctt atggcagatt
    13801 ttattttagg gcttatatca agaagtgtac ctcaattaaa tgtaatgata gttggtatgc
    13861 cattaaaaat attggttggt attgcattat ttatcatatc aataccttta attgtcaatc
    13921 aaataagtca tttattaagt caaataccaa agatgtatga gggaactttt gcactagctc
    13981 ctatgttttt tatgggttca acagataaaa cagaagaagc tactccaaag aaaaagggag
    14041 aacaaagaaa gaaaggtaat atagcaaaga gtagagaatt acccgttgca atgactctac
    14101 ttgcattcac cttattagta cctacattat tttcatatgt agtagataca cttaaatctt
    14161 cgcttaatta ttttttgagt ctagattttt atatgaacat aaattattca aacctagaga
    14221 aattagttat agcaggatta atggattttt ttaagatatt tttaccaata gctattccct
    14281 ttttagtttt aggcataata gcaaacctat ttcaggtggg aatactgttt actggtgaaa
    14341 ctttaaaacc caatttatca aaattaaatc caattagtgg attcaaaaac atgttttcta
    14401 tgagaagttt gtcaacctta ataaaagata cagcgattat atctattttg gcttacatag
    14461 gatatacatt cttccaagat aattatttag atatattaaa gctaggtaac atatacctac
    14521 caactttaat gtacacagtt aaagatttag tatattcaat attaagtaag atttgtgtag
    14581 ctatgatagt tatagcagtt gcagattatg tttatcaaag atacagtcat aaaaagcaac
    14641 ttagaatgac aaaacaagaa gttaaagatg aatataaaaa ctcagaagga gacccagaaa
    14701 taaaagctaa gataaaacaa aaacaaagac aaatttcgtc tcaaagaacc atgcaagcag
    14761 taccaagtgc tactgtaata gtaaccaatc caacccactt atccatagca gtgagatatg
    14821 aaaaagggaa agaccaagct ccagtagtgg tggcaaaagg tgcagattat ttagctttta
    14881 aaataaggga aattgcaaag ggaaatgaca tccctataat agaaaacaaa cctattgcta
    14941 ggttgttata taagcaagta gaaatagacc aagaaattcc agaggacatg tatcaagctt
    15001 ttgcagagat attagtggca gtatataaaa tcaaaaatag atacaaagtc cccaaaaggt
    15061 aggataagta atgaataagt ttaatttaaa ggaaaatatg gatgtaatag tggcatttgg
    15121 tgttgtaggg attgttctta tgatgatagt gccattgcct acatttatat tagatatttt
    15181 actggcaatt aacataagtt tatctgtgct aattttacta atgacattat ttacaacaaa
    15241 tgtacttgat ttatctatat ttccaacagt gttacttgtg acaacactat ttagattggg
    15301 attaaatctt tcatcaacta gattgatttt aacacaggga tatgcaggtg aagttataga
    15361 ggcatttgga agttttgttg taggtggaaa ttacgtagta ggaataatta tattcttaat
    15421 tatattaatt gttcaatttg ttgttataac caatggttct ggaagagtat ctgaagtaac
    15481 agctagattt acgttagatg ccatgccagg taagcagatg agtatagatg cagatttaaa
    15541 tgcaggagtt atagatgaca aaactgctag aaaaagaaga aaagagcttc aacaagaagc
    15601 agatttttat ggttctatgg atggagcaaa taagtttgta aagggagatg ccatagcagg
    15661 tcttatagta acagcaataa acataatagg tggaattgta ataggagctg taatgctgaa
    15721 tatgactctt atgggtgcag cacagacata tgtaagactt actatagggg atggtcttgt
    15781 aagtcagata cctgctctat taatatctac atcagcaggt ataatcgtaa caagagcttc
    15841 tactgatgaa aactttggtg gattagtagg taaacagtta acggcaatac caaaagcagt
    15901 aatgatgact ggttgtgttc taatcgtact tggtatgatg cctggattac ctaaaccagc
    15961 atttttttca ctaggtatag gagctatagc agcaggttac tttttaggaa gagaaaagaa
    16021 agatacttta gaagacttgg agtttgaaaa acctgatatg gtaaatccag ataaaaatga
    16081 agtagaagat gtaacaaatt taataaatgt agaatcaata gaagtagaaa ttggatatgg
    16141 tttaatatct ttagctgatg aatcagcagg aggagattta cttcagagaa tagcatcaat
    16201 aagaagacaa tgtgcaatag acatgggtat agtcgtacaa cccataagaa taagagataa
    16261 cttacagtta aatccaaacc aatacaatat aaaaataaaa ggaaatgtag ttggtgggta
    16321 tgagataatg cctaatatgg ttttatgtat ggatcctatg aatcaaggat ttagtatatt
    16381 tggaataaag acaattgaac ctacttttgg aatagaggca ctttggatag aaagtgacaa
    16441 ggtagaagat gcagagttaa agggttatac agttgtagat tcatccacaa tacttataac
    16501 tcacttacta gatataataa aatcaaatgc acacgaatta ctgggaagac aagaagttaa
    16561 gactattata gatgcagcaa gagaaaatta tagtgctgta gtagatgaat tgataccaga
    16621 tttgatgaca cttggtgaaa ttcaaaaagt acttaaaaat ctgttaagag aaaaagtaaa
    16681 tataaaggat agagttacaa tacttgaaac gcttgcagac cagtctagaa atacaaaaga
    16741 tatagagctt cttactgaat atgtaagaat agccttggct agaagtattt gtaataattt
    16801 agttgatgat gataagacaa ttgtagtggc tacattatct ccagaaactg aagagttggt
    16861 atctaataat ttacagaggt ctgtaaatgg aacttatcca gcagttgacc cagaaaatac
    16921 aaataaaata tttgaaagta tacaagaagt aatgagcaat gtatatttca atcataatat
    16981 accagtaata gtagtatcac caaagataag agctccattt agaaaattag ttgaaattgt
    17041 atatcctaat ttgactgtat tatcattaaa tgaaatacct aacgatatta aaatcaaagc
    17101 agaaggtgtg gtgagcatat atgatgacga aaaagtatac agctaacaca attcaggatg
    17161 ctatgaatct agccaaaatg gaattgggtg ataatattac attaatagat aaaaaagaag
    17221 taagaaaatc tggaataaga ggcatttttt caaagaaaga tattgaattg actattggat
    17281 gggaaaagaa agataattta gaacaaaaag atttaaaacg agagatagaa caactaaagt
    17341 caattataaa taatatgggg tttgataata aaaatgataa tgatatagat aaaatatgca
    17401 agaatttact aaacttagat ttaaatcaag aaattgtaga atttataaaa gctgatttac
    17461 aagaaatgaa gttcaatgga attgatacaa gtaaaaattt agtagaaagt ctaaagaaaa
    17521 agattaaaat agaaaatcaa gcaataaatg gtaaaattgc tttagttgga ccaccaggag
    17581 taggtaaaac gactacaata gcaaagttgg ctgccaaatt agtttttgag gaaaataaaa
    17641 aagtaggagt aataactata gatacataca ggattggtgc tgtagaacag ttgaagatat
    17701 atactgatat aatgaatatt ccctttaaag gggtaataag tcctgatgaa atggagttgg
    17761 cacttgacga gatgaaagat tgtgatgttg tattaataga tacaacaggt agaggctaca
    17821 aaaactctat gcaaatacta gaaatcaaaa atcttataga taaagctgaa actgataata
    17881 tacatttagt tttgaattgt acaacaagag aaagtgatac aaaagcaatt atagattcat
    17941 atagaaatgt gaattttaaa agcttaataa ttacaaagct agatgaaaca ataacatatg
    18001 gctcaatatt taacataatg aattatgcac aaaaaccaat atcatatatt acaacaggac
    18061 aaaatgttcc tgatgatatt ataaaaccta atgaagataa gattataagg ttactattag
    18121 gagttgaaag tatatgatag accaagctca aattttgaga aaaatggcaa tggaaaaaag
    18181 aggacttgat gagtttatag tagagaatga taatgtacct aagataatta caatagcttc
    18241 tggtaaaggc ggtgttggaa agagtaatct cgctacaaat ttgtctatat gcttgaccaa
    18301 acttaataaa aaagttctta tattagatgc agacatagga atgtctaata tagatataat
    18361 aatgggggta aatgtaaaag ggactataat tgatgtaatt aatggcgaaa aaaatataga
    18421 agatataatt tcacagacta agtatggtgt aaatataata tctggaggtt ctgcccttaa
    18481 tcatatagaa gactttacag aagctcaaag aaataagttt atacatagca tagaacagat
    18541 tcacgatgtg gattttataa taattgatac aggagctggt atgagcaaaa gcctattgtc
    18601 atttatatac tgtagtactg aatttttctt aataacaact ccagaaccga catcattgac
    18661 tgacgcatat agtttattaa aagctataag taattttggc ataaagagaa gtgcaaatat
    18721 aataatcaat agggtaattg atttagaaga agcaaaatct acatacaaaa gaataaaaat
    18781 ggttgtggac aaatttctaa accttaatct agaattgtat ggatatataa tagatgacaa
    18841 aaaagtaagt ttatgtgtaa aaaaacagat accattcatt ttagaatatc ctacaagtat
    18901 tgcatcaaaa tgtattatag ctatagcaaa gagaatggta agtcagtcaa gagaagatat
    18961 tgttgataat aaaagttttc taagaaagat gtttagtata tttaaaagct aaggaggcgt
    19021 agttaatgaa tagagaagaa ttaataaaag agaatatgcc tcttgtaaag agtatagcaa
    19081 gaaaattttt tataccaggg aaaggttttg aatatcaaga cttagtaaat agtggagtca
    19141 ttggattgat tgatgcaata aataaatttg atgctgaaaa aggggcaaag ttttctagtt
    19201 actcttatat aaaaataaaa tctgctatat tagatgaaat tagaaatcaa agtccaattt
    19261 ctaaacataa tctaactaaa gtaaataagt ataatagagt ggtagaaaag ttgcaatcta
    19321 aattattaag agagccaact tcatatgaaa ttgcaaaaga attaaaggtt tcagaaaaag
    19381 aacttcatga tatcgaaagt aatatagata tgttaaatat tgtatcatta aactatgtaa
    19441 tctttgaaga tacaaatgaa actgtacaag atgtaataag tgatagagaa gaggaagctc
    19501 cagaaaatat aatagaagaa gaagaaaagt tagagattct atctaaagca attagtaatt
    19561 taaatgaaag agaaaaatta atactttcac tgtactatta tgaagattta aatctaaagg
    19621 agatagggaa agtgctaggt gtttcagaat caagagtttc acaactccac agaaaatcaa
    19681 taagaaactt aagaaacaag ataaaagaac ttaaatattc tatatagatg gtgattgata
    19741 agatgataca aattatatta atagtagtgt ctattttaat aatagtaact atagttataa
    19801 atttacataa ggaatcaaaa aataaagata acagatactt tgataaaata tttgagcaag
    19861 aatatgaagc aaatgataga ttaataattg cagaaaaaag aagattcttt ttagacaaag
    19921 tacttgcaga aaaaagaagt aataatcata taaaagttta tgaaaattat aatctaataa
    19981 aggctaatga agaaagaaag gaaaacatat ttatagaatc aaaaaaggaa gtaagacaag
    20041 aaaatgagtt atctcttaaa gtgaaacatc ttataagttc aggttatgat gatgtggaaa
    20101 tatgtaagat gctagatatt ggaaaggggg aactgttatt aataaggagt tgttacaaaa
    20161 tataaggata ttattatgtg ataaaaaagt attcttagga ataggtattg gtatattaat
    20221 atcaacttta tgtataatgc tttttagtaa taatgatatg agtaaggcag agatagaaaa
    20281 aaaggccaga gcgttaggca tgaaatatac aagtgagatg aaagttttag aatagatgat
    20341 actgtttaga catttacaaa aaaggaggtc ataatgtgat aagaggatta tatacagcag
    20401 tttctgctat gataactaat caacaaaagc aaaatgttgt tgtcaataac ctagctaata
    20461 tggatacaaa tggatataaa agtaaacaac ttttgactaa aagttttgat gaattaaagt
    20521 tggagggtta caataactac gcaaatggaa ctaaacaaaa gcagattata ggcgatataa
    20581 gtccaggtat ttctatggat gaaactatca caaattttaa tcaaggacct ataaaaacta
    20641 ctgataataa aatggatgta gctattcaag gtaagggatt ttttctagtt agtgatgcag
    20701 ctggaaatca attttacact agaaatggga acttcagaac agacaatcaa ggtgacctta
    20761 taacaagtga agggtattat gtacttggga cgaatactgc tactggtgct gtaggtcata
    20821 taaatgtagg aaatcagaaa tttgaagtgg caaaagataa tacaatatca ctagacggaa
    20881 atgctatgta taaatttaat attgtagatt ttaataacta caataactta aaaaaagaag
    20941 gagataactt atattctgga ggaggagcta tgaaagtaaa taatgcactt acaatacaaa
    21001 gtgcgataga gtcctctaat gtagatatgg tatctgaaat taataatatg atgacaatat
    21061 caagagaata tgaggcaaat caaaagataa ttcaggctat ggattctaaa ttagcaaaaa
    21121 tagcaagtga gattggttca gtaagatagg taataggagg tgaattttta tgtacacaat
    21181 gttttatact agtaaaacag ctttatcagc aaatcaaagt aaactagata taatatctaa
    21241 caatatagca aattctgatt caacaggata taaaaaaata aatatggaat attctgattt
    21301 agtggatgaa gtactaaata gaccatcata tcctacaaat ggcaatgata tatcgactgg
    21361 aacaggagct aaggcaacag ctcccatgag aatttatgaa caaggtgcat tggctcctac
    21421 agatagtaag agtaatttgg cgatagatgg agaaggattc tttagagtga taagaaaaga
    21481 tggtacatat gcatatacaa gaaatggtgg atttaatgta gatgctttag gtaagattgt
    21541 agatgataat ggaaatatat tagatgttca atttggtgca ggatataact ataataatac
    21601 gaatatagat agtacaaacc tgacagtttc aagaaatgga gagctattcg tagataataa
    21661 aaaaatagga aaaatcaact tatatcaacc tataggaact caaaatttta tatctgaagg
    21721 agatagttta ttcgtcgcaa ctgatgcagc acagattaaa caagttgaga aagtagatat
    21781 agttcaaggc tatagagaga ggtcaaatgt ttctttacaa gaagaatttg ttgacttaat
    21841 agccactcaa agagcatttc aaatgaactc aaagggaata aagacagcag atgaaatgtg
    21901 gcaaatagcg aataatttaa gagctaaata attatgtaag gtgaggaata ttcatgaatg
    21961 atatgaaaga gtacgatttt ggcaaacctc agagttattc aagagaaaat ttaacttgtt
    22021 taaatttttt attagctgaa ttttgtaaaa aatataaaaa ctatataatt tatgaactta
    22081 agtgtaattc tagtatggtt gttgatagta tagaccaagt aaattatcag acttttttag
    22141 atagagtaac ttcttcatgc gtagtagtac aaaatgcaat ggaacctgtt atgaaaaact
    22201 tttcttttag aatagataga agtgtagctg atatgtggat tgatataact tctggaggaa
    22261 ctggagttgt aaaggataat gatagagagc ttacagaact tgataaaaaa gtgcttttac
    22321 accttatgga tgatttagta agaaatatgc acttatttga gggttttgaa aagatacaag
    22381 tattggatac acatacacat attaatttgc cacaattatg ttctccaact gcaccagtat
    22441 gcgttgtaga tttaaaagtg ctaaatgata atgagtatat tggaagtgtt tctctttgtt
    22501 ttccatacaa tggtttagag gctctgttag aaagtatatc tgttatagaa ttttttgata
    22561 atgatgtagg agatgattca gaggaattta cccaaaaaat ctataatagt gtatctaata
    22621 tagaattatc agttattgca gagataggaa aagtatcaat taatgttgag gatttactta
    22681 aactagaagt tggagatgta atagtaacaa ataaaaaaat aaatgattat atagatatat
    22741 ttgtagaaaa ttcaaaatca tacacagcaa caccaggatt tatatcaagc aaaaaaggtg
    22801 taaaaataaa tgatgctgtt ggaaaagagg tgtagttatg gacaatagta atcttatttc
    22861 agatgaagaa gcaaagatgt tattaaatga taaagttgaa agtgaagttg atggagatga
    22921 tgagttagcc ttagatgtct ctgaagttaa tcgaaataat atacaaagga tacttgacct
    22981 taagttagaa ttatctgtag ttataggaag aactaagatg actctaagag atattttaaa
    23041 tttacataaa ggttctctta ttgaactgga tactttagca gaccaagatg tggaaatact
    23101 aatagataat aaggttttgg cttatggaaa agtagtggtg gtagatttaa attttggtgt
    23161 aaagataaca agtatagtaa gtgaagaaga catggtaaaa tctcttgttt agagataaca
    23221 tcaattaata cttaaggcat gaggtggtac ttattttaaa cttaaatgat ataaaaaaag
    23281 atatactaaa actcaataaa aaagactcat gttctctata taatgaatct attaataata
    23341 taaataataa ttgtatatca actggattaa aatttttaaa acaagcaaga gatttaaacc
    23401 cagatgatgt tgatatatta aatctcacag gtcttgtaaa cttactaaaa tgtaattttg
    23461 atgatgctgt agaaagtttc tataagagtt cactttgtga aaaaactgct ctgtgtgaaa
    23521 agtatataaa catgcttaca tctgaggagt ttttaatttt ctttgaaaag tacaatcaac
    23581 ttataaggtt aataaataaa ggaatatata aagaagctat acaagggttt aaacttataa
    23641 ctgaccaata ctgtgattta atagaacctt atgagttgct tgccttactg tatgacaagg
    23701 aagaagaata tataaaattt gatgaatgtt tagaagtatt gaagattatt gataaggaaa
    23761 attatttatt aaatcaagaa aataacatat tagatatgta taatagtgct aatatacaaa
    23821 ctaaaagtaa tataaaagta gatgattata agaatgagtt tttacataaa agtagtaata
    23881 accataagat taatgattat aataatgtaa ctaaagaaaa gagtttaagc aaaaaacaga
    23941 aaagttcaga ttctaataag aaaaaaagta ttaaaaaaaa taagtaccta tatatagcca
    24001 taatattagg aatattatta gtaggacaaa gtatatatag ttcttataag atgaataatt
    24061 taactgataa aatatctaaa aataataaaa ctaaaaatga agagtcaata gattctaact
    24121 tgactaagaa agaaaataat atagaagaaa atgaagatat gagtaaggct tctaatataa
    24181 ataagagtaa taaaaacatt aataaagagg atgatgtaaa atctaaaaaa aatactgtag
    24241 aaaatgaaga tgataaagaa tctgacttat ttacagagga tgaattgatg tctaaggcaa
    24301 atactttaaa atcagaaaag aaaactaaat cttctataca actatataag aaagttgcag
    24361 atataggtga aaataaaggg aatacatctg aagcaactta tcaagttgct attctcagtg
    24421 aaaagttaaa agactataaa actgctgaag aatattataa aatgtatgta gaaaattact
    24481 cagaaaaaga tgcttatttt gatgaatctt attataattt aggtatgatg tactataaca
    24541 atggtgattt aaaaagttct aaattgacat taaggaagtt ggttaataag gtaccaaata
    24601 gtatgtataa taattcaaaa gtaaaagaaa ttttaaaaga agaatagtta aattagataa
    24661 gaaaaataaa ttattttatt ttaaaataat atgattataa tatattgaca taccatttta
    24721 aaaaatttaa aatattaatt ataaaatgag gagggataaa aaatggaatt tgaaaaaggg
    24781 agtatttcaa ttcatacaga aaatattttt cctataatta aaaaatggtt atactctgat
    24841 aaagacatat ttataagaga gcttataagt aatggatgtg atgcagtaag taaacataaa
    24901 agacttgtat cactaggaga aatatcagaa aataagtcat cagattataa aattacagta
    24961 tctgtaaata aaggagaagg tacacttaaa tttattgaca atggaattgg tatgactgaa
    25021 gaagaaataa aaaaatacat aaatcaagtt gctttttcag gtgctgaaga tttctttaat
    25081 aaatataagg ataagatgga agagtcaaat gacataatag gtcattttgg attaggattt
    25141 tattctgcat ttatggtgtc taaaaaggta caaatagata cactttcata tacagaagga
    25201 gcgactcctg taagatggat atctgaaggt ggtacagaat acgaaatatc tgaatcagat
    25261 actagaaatg atagaggaac tactataaca ctatttattg atgatgatag taaagaattt
    25321 ttagatgaat ttactgtaag aggaataata aataaatact gttctttctt accagtagaa
    25381 atatatttag aagatgttga aagattagaa agagaagcta aagaagcaga agaaaaagct
    25441 aaaaaacaaa aggaagatgg aaaagaagag gttgtagatg caaaagtaat tgagccttta
    25501 aacgacacta atcctctatg gttaaaatct ccaaaagact gtactgatga ggagtataaa
    25561 gagttttata gaaaggtatt ccatgtattt gatgaacctt tattctggat acatttaaat
    25621 gtagattatc ctttcaactt aaagggtata ctttacttcc ctaaattaaa aaatgagttt
    25681 gaattaactg aaggaaaagt aaaactatac aacaatcaag tgtttgtggc tgacaatata
    25741 aaagaagtta taccagaatt tttactttta ttaaaaggtg ttatagactg cccagactta
    25801 ccattaaatg tatcaagaag tttcttacaa aatgacagag acgtaagcaa aatttctaag
    25861 catataatta aaaaagtagc tgataagtta aaatctcttt gtaaaaatga aagagaagaa
    25921 tacaataagt tttgggatga tatacaaata tttattaaat atggttgctt aaaagatgaa
    25981 agtttctatg agaaggtaaa agaatgtata ttattcaaaa ctattgatga tgagtatata
    26041 acattacaag attatttaga aaaatgtaag gacaaacatg aaaataaggt gttttatgta
    26101 agtgataagg aacagcaatc tcaatacatt aaactattta aagaatacga cttaagtgct
    26161 gttgttttaa actcatcaat agatactcat tttatttcat ttatggaata taaagagaat
    26221 ggagttaagt ttaatagaat agatgcagac ctttctgatg tattaaaaga taaaaatgaa
    26281 aataaagata gtgaagaaaa caaagaagag atagctaaga tagaaggtct gtttaaagaa
    26341 gctgtcggag aaagagtgaa gaattattct gtagaaggat tgaaaaatga agacactcca
    26401 gctatggtgt tagtatcaga acaatctatt agaatggcag aaatgcagtc aagatttgct
    26461 ggaatggatt taggaatgaa ttttgaggaa gaaaagactc ttgtgataaa tgaaaacagt
    26521 ccaataatca aaaaattagt ttctcttaaa gatgatgatg aaaagaaaga taagattacg
    26581 cttatttgta accagatagc agatttagct ttactttcta ataaagaatt aaagccagat
    26641 gagttagatt catttgtaca aagaagtaat aagcttatga gtcttttgat agaattataa
    26701 ggttgaattt gaatacattg aagcaaattt ataaaaagta ttcaatatca atgataataa
    26761 agaagtgtct caaaatgaac ttttcagttc atgaggcgct tttttatgtg aaattaaatt
    26821 tattttaatc atttcaacaa tgaaaaatat atttatctct attttgagac agaccattct
    26881 ttattttttt atctttggtt aatttttaaa tatttaagta ggttctaata aaaaactcta
    26941 tttagttaaa tatagttaac ttgatatata ataagataat acatataatt aagtaaaaga
    27001 ttgtttttta gtttatgttt aaatacataa ttacactttg ttaaaaatat aaaattatta
    27061 ctaaagtaga aataggaggt tttagcatga attttaatta taatttgcca gtaaatatct
    27121 tatttgggag aggcagaatt aatgatgtgg gaaaagaagt atctaaatat ggcaaaaaag
    27181 tattgatagt aacaggtaaa aatagtacaa agaaaactgg attacttgat aaaacaatag
    27241 atttactaaa agactctaaa atagaatatg aagtttttga tagagtagaa caaaatcctc
    27301 ttataactac tatatattca ggagtagaaa ttataaaatc aactggatgt gattgtgttt
    27361 taggtcttgg tggtggaagc attatggatg ctgccaaaag tatagctttt tcttataaaa
    27421 acccaggaga tttaaatgat tatatatttg gaattagaca aggtgaagaa gctttaccta
    27481 taatcttagt tccaacaaca tcaggtacag gtagtgaagg aaattgtttt gcagtactta
    27541 caaattcaga aactaaagat aaaaaatcta taaagaagaa tagtatgtat gcaaaagtat
    27601 caataattga cccagaactt atggttacaa tgccaaaaca tataattgca tcagttggat
    27661 ttgatgcact tgcccataat atggaagcat atctttctaa tggaagaaac cctcttgcag
    27721 atgtacaagc tatttatggg attgaactta tatcagaaaa cctaataaag gtatataatg
    27781 atgttaatga tttagaagca tgggaaaaga taacacttgc aagtactatt ggaggaatga
    27841 cgattggtac agcgggtaca gccttgccac atggaatgga gcatcctgta agtggtctta
    27901 gagatgtaat gcatggaaaa ggacttgcag ctttgactcc tactattata gaaagttcat
    27961 gggatagtga tattgaaaga tttggtaata tttcaaaggt attaggagga acttgtgcag
    28021 aagattgttc agatgcaatt agaaatttcc ttaaaaaaat agatttagat ataaaactaa
    28081 gtgatttagg tattgaaaaa tcagatatag attggttaac tgataattgt atgaaaatat
    28141 cagttgcaaa tattaagaga catccaaagt attttaataa ggaacaaata aaggaaatgt
    28201 accataagtc tttataattt gactttacat atttagtttt atataaaaat tataaattaa
    28261 taataaaaaa ttataagtaa ttaatagtaa aaaaattgtg tatagaagtt atatatataa
    28321 taaacttgtc ttaagctttt attttacttt ctaataaata aaggagttta aaaaagggaa
    28381 gattaattaa gtaatatttt ataaaattct tcgatttaaa aactttaagt agaggtgaaa
    28441 taaatgttta atcccaaaag aataatattc gaaaaaggaa ctttagacac tgatgtgggt
    28501 aaaaatatat acaataaatt tagagataat aaagatgtag agataattaa tatatcatca
    28561 aatagaatta aagaacatat tccaggtgaa aatttatatg aacaatatag aagtggtaag
    28621 cagactatgg tagttgggat tagaaaaagt ctgaagtttc aaacatgtaa accttctgct
    28681 aattatcaac taccacttgt cagtggatgt atgggaagat gtgagtattg ttatttgaat
    28741 acacaattag gtgataaacc atttataagg gtatttgtaa atgtagatga aatacttgaa
    28801 cagacaaaaa aatatattga agatagaaag ccacaagtaa ccatatttga aggggcggct
    28861 acatcagacc cagttccagt agagccatat actaattctt tgaagaaaac aatagagttt
    28921 tttggaagag aggaaaaagg aagatttaga tttgttacta aatacagtga tgtggatagc
    28981 ttattggatg ctgaacataa taatcataca gaaatacgat ttagtatcaa tactcctaga
    29041 attataaatg aacatgagca ttatacagca tctgcaaata aaagaataga gtcagctgta
    29101 aaacttgcta gcgctggcta tcagattggt ttcataatag cacctgtgtt tttatatgaa
    29161 ggttggaaag aagagtataa aaatctgatt ataagtataa aagaagcttt accaaagggt
    29221 tttgataaaa aaattatatt tgaagttata tctcatagat atacaactaa agcaaaaaat
    29281 agaatactag agatttatcc agatacacta ttgccaatgg aaaatgagga tagaaagttt
    29341 aaatacggtc aatttggata tggtaagtac gtgtatgata aagaacaatt agatgatatg
    29401 aaagagttct ttcttaagga aataaatgga gtatttaaaa atagtttaat aaaatatata
    29461 atatagcaaa tcttacctaa agatttgcta tgttttattt ttatatattt tatgtttata
    29521 ataaagctat gagatttagt attatcatat aagaatatta aaaagggcac tatctatcaa
    29581 gaacattttt tctagttttg atactataaa ggataagagt aaccaaaata tcaggaggag
    29641 ttataatatg catgcatggg agtctattca aattacattg gatttaatag aagcaaattt
    29701 gtcagaagaa atatcaatta atgaattggc aaataaagca aatttatcac cattttacta
    29761 tcaaagacta tttaaaagat tggtaaataa gactgtaatg gagtatataa agcttcgtcg
    29821 tcttgcaaga gcttctgaat acttagcaga acataagaat cgtatacttg atgtagcttt
    29881 aaattttgga tttgctagcc atgagacatt tacacgttcc tttaaaaaag catatggatt
    29941 aactccagaa aaatatagag caaatccagt aaaactcaat cactttatta agccagaatt
    30001 aattttaaat tatgctatgg ttgatgaagg tgttccactg attgcagatg atatagtgat
    30061 tgaagtcaat cgtaagacat tgagtaatcc aaggacgttt gttggaatag aaatagaagt
    30121 acctatttgc cagttggtgg gtggtgaaac aactggtatt gcaatagtag aggaattatg
    30181 gatgaaactt ggaagtcaaa gacataatat accaaatcaa attccaggtg gaaatgaatt
    30241 tgctgctctt tatatgggaa atgcaaaaga aggaaattgt atgtatatgg caggtgtaga
    30301 ggtagagtca ggtacatcag tagaaggata tagtactttt gagttaccag ctaaagaata
    30361 tttagtatgt ggatttgagg cagaaaactt taatgagtta gttaattcag ctgtttttaa
    30421 agcggataaa tttatggaaa gatggatgaa gaaacataat ttaacaacta ctgattttgc
    30481 aattgagatg tattacccca ctacacctga agcagcatat ctggaacatt ggattgttcc
    30541 ggttcctatt gaacaataga aaggatgatg aatatgaatt ggcaagatgc gttttttcaa
    30601 gatactaaac ctacatttga gcaaattaat gaatttatta ataatccatt atgggacaag
    30661 ttaaataaaa ctttggtttc tacttataat gtagaaccta agcttgaata cagtaaatgt
    30721 tcaatgcaaa gaggttggaa tgtaaaatat aaaaaaagag gtaaatcact atgtacttta
    30781 tatccacaag aaggttattt taaagctttg gttattgtaa gtgaaagcaa tagagtagag
    30841 gttgatttat ttattaatac ttgttgtgat tatatcaaaa aaatatataa tgaggtaaac
    30901 ttttttaatg gaagtaaatg gctaatgatt caagtagata gtttattggt attaaacgat
    30961 atgcttgaac taattaaatt tagagcttaa catatagaac aggatgaata tataagtcat
    31021 cctgttttta tattcaacca gataaattaa tatatagtct ataatatgaa taatttagta
    31081 aatttaaatt tactttatct ttctacattc tttgcaacta agtccacatg tgtttttttc
    31141 ttgcatatta gtagcacccc aattagcaaa ttgttgtaaa ataggcacaa tacttttacc
    31201 tttgcttgtt aatgtatatt caacttttgg aggaatttca ttatattgtt tacgatgaac
    31261 aataccagta gactctaaat cttttaatga ttgagctagc atagtatttg taatgccatc
    31321 caattttctt tttatgctac tatatctaat tggattttca actgctaaca tacatataat
    31381 aggtaatttc cacttaccac cgacaatgtc taatgcataa cggattggac aggttaattc
    31441 ttcaacgttt ttagttatat ttttttcagt atttatactc attttaatta ctccttagtt
    31501 atagtagtat cttttttata ctttatcata aaaaactgca tacttgtttt ttctgtattt
    31561 tatattaaaa ttatatcaaa ggataatcct taatacaagt atttaaaagg ggtgtaagaa
    31621 ttatgaaagc attagaattt ttgaacaagg cacgagttta ttatttagca acagttgatg
    31681 agaacaatca agcacatgtg cgtcctttag gatttgttat ggaatacaat ggtaaactta
    31741 cattttgcac atctaatcaa aaggatatgt ttaagcaact tatttttaat ccaaaggttg
    31801 aactttgttg tatagaccaa aaccttaata ctctacgtat tttaggtgaa gcagtatttg
    31861 ttacatcaaa agaaacacaa gctaaggcac ttgaaataat gccaagttta ggtcagatgt
    31921 actctgtagg agatggtaag tttgaaatat tctctattga taaggcaaaa gttagttgtt
    31981 gttcaatgag tggtaaaaag attgatgtag agctttaatt ataattgaat cgacatccat
    32041 agtgaaaatt aagaaaacgt tattactgaa atattgatag atttaattta aatgcaataa
    32101 aaagattttt aaataatgaa tatgtaaaaa tttttagtta ttagatgtgt atttcaataa
    32161 tgatatacta ttttgaataa ttacatacta gaaataagat aattaaaatt tactttataa
    32221 tgggatagat ttcttttaaa aaatatttta actataattg atttttattt aatctgaaaa
    32281 aaatattatg actataaaaa attgctactt attatttgga tatttttgta tataaataat
    32341 aagtagcatg actttataca ttaataatga
//
LOCUS       DAEMHW010000027        31937 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEMHW010000027 DAEMHW010000000
VERSION     DAEMHW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.027"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            333..446
                     /locus_tag="KPK67_001039"
     CDS             333..446
                     /locus_tag="KPK67_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2458036.1"
                     /translation="MIMGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            448..1566
                     /locus_tag="KPK67_001040"
     CDS             448..1566
                     /locus_tag="KPK67_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2458037.1"
                     /translation="MVVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKVRDKQVPKGRILNATISKEPSGR
                     YYVSLCCTDVDIEALENTNNQIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IKNHKLSRSISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKN
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            1717..2934
                     /locus_tag="KPK67_001041"
     CDS             1717..2934
                     /locus_tag="KPK67_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBG2458038.1"
                     /translation="MLIGLIAAFGLVLQKKKASDVIKGSLLAAFGIIIMETGTGMLVS
                     SIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAIHLMIARFTPIKTIF
                     LTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPWLLRKYVFDVIGDDS
                     FTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREISISGGMAVFLMFIVV
                     GIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQIMPAFQGISEKVVPN
                     ALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNLLLIPLVITSFFECG
                     AAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNWILIFGGNDLSLFGI
                     ISKFIGSLFGGIL"
     gene            2937..3677
                     /locus_tag="KPK67_001042"
     CDS             2937..3677
                     /locus_tag="KPK67_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2458039.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            4000..5310
                     /locus_tag="KPK67_001043"
     CDS             4000..5310
                     /locus_tag="KPK67_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG2458040.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            5408..6325
                     /locus_tag="KPK67_001044"
     CDS             5408..6325
                     /locus_tag="KPK67_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG2458041.1"
                     /translation="MENLVREFEENSKRKKTKINIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            6341..7150
                     /locus_tag="KPK67_001045"
     CDS             6341..7150
                     /locus_tag="KPK67_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG2458042.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            7208..8956
                     /locus_tag="KPK67_001046"
     CDS             7208..8956
                     /locus_tag="KPK67_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBG2458043.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNIPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            9218..10240
                     /locus_tag="KPK67_001047"
     CDS             9218..10240
                     /locus_tag="KPK67_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBG2458044.1"
                     /translation="MFGGIKIKRIGIIIMFFLSIAFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSNNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            10245..12550
                     /locus_tag="KPK67_001048"
                     /pseudo
     CDS             10245..12550
                     /locus_tag="KPK67_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            12522..13637
                     /locus_tag="KPK67_001049"
     CDS             12522..13637
                     /locus_tag="KPK67_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458045.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLNSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEDKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSSMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSINKKDKDGVPYTLVSPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            13655..15073
                     /locus_tag="KPK67_001050"
     CDS             13655..15073
                     /locus_tag="KPK67_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG2458046.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            15088..16296
                     /locus_tag="KPK67_001051"
     CDS             15088..16296
                     /locus_tag="KPK67_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458047.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(16370..17758)
                     /locus_tag="KPK67_001052"
     CDS             complement(16370..17758)
                     /locus_tag="KPK67_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG2458048.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            18098..19432
                     /locus_tag="KPK67_001053"
     CDS             18098..19432
                     /locus_tag="KPK67_001053"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBG2458049.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(19561..20478)
                     /locus_tag="KPK67_001054"
     CDS             complement(19561..20478)
                     /locus_tag="KPK67_001054"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBG2458050.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            20686..21570
                     /locus_tag="KPK67_001055"
     CDS             20686..21570
                     /locus_tag="KPK67_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBG2458051.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQT
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            21802..23643
                     /locus_tag="KPK67_001056"
     CDS             21802..23643
                     /locus_tag="KPK67_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HBG2458052.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFNNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPILKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIFSSTLICLLNFNQKELKDKKSK"
     gene            23948..24655
                     /locus_tag="KPK67_001057"
     CDS             23948..24655
                     /locus_tag="KPK67_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2458053.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            24649..25959
                     /locus_tag="KPK67_001058"
     CDS             24649..25959
                     /locus_tag="KPK67_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2458054.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNPIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMAQE
                     NYLKDTTESEETGYLVEYNEYFKEGTFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKSEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            26076..26759
                     /locus_tag="KPK67_001059"
     CDS             26076..26759
                     /locus_tag="KPK67_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2458055.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDEPFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            26752..29139
                     /locus_tag="KPK67_001060"
     CDS             26752..29139
                     /locus_tag="KPK67_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2458056.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILARIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            29419..30561
                     /locus_tag="KPK67_001061"
     CDS             29419..30561
                     /locus_tag="KPK67_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG2458057.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKVPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            30580..31104
                     /locus_tag="KPK67_001062"
     CDS             30580..31104
                     /locus_tag="KPK67_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2458058.1"
                     /translation="MEVTVKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGGKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            31204..>31937
                     /locus_tag="KPK67_001063"
     CDS             31204..>31937
                     /locus_tag="KPK67_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2458059.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSQLFAKKLEESLNPTQSVEKIVLYLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKE"
BASE COUNT        12545 a         3320 c         5607 g        10465 t
ORIGIN      
        1 tgagggaagt acactacaag ctattggtag tggaagtggg tagaccttag cggataatgc
       61 cgaaggataa gatgtagtct gtgcttacat gaaagtgtaa gaagttcata agagaactgc
      121 ataggtagta gcgaacttat gtgaacgaca acctcaaaaa tgataaaagt taagacttac
      181 ggttcttata tatgttattt aaaaataaat ataaaaaacc ttcaaaatac ttgttttaag
      241 taattgcata ttttaatatt atgataagtg atgaaaaatg gaagttactc atggtagagg
      301 atatgcatat agtatgtcaa atatagacat ccattattat ggggagacat ctttggaatc
      361 ctagttattt cgttgctact gtatcagaaa atacagagga gcagataaga aaatatatta
      421 ataatcagaa aaaacaagta aagtgaggtg gtagtagtgg aaaaagctta taagtttaga
      481 atgtatccaa ataaaaaaca acaagagtta attaacaaaa cttttggatg ttgtagattt
      541 gtatataata agtatcttgc taaaagaata gatgtttata aaaacgataa agaaactttt
      601 acttataaac agtgtagttc tgatttgacc aatttaaaaa aagaattaaa ttggcttaaa
      661 gaacctgata aattttcact acaaaacgcc ttaaaagatt tagataatgc atataaaaaa
      721 ttctttaaag aaaaggcagg atttcctaaa tttaaatcaa agaaaattaa tagattttca
      781 tataaaacta actttacaaa tggaaacatt atgtattgtg gtcaacatat aaaactacct
      841 aaacttggta tggttaaggt aagggataaa caagtaccta aagggagaat acttaatgct
      901 actatatcta aagaaccaag tggtagatat tatgtatcat tatgctgtac tgatgtagat
      961 attgaagcat tagaaaatac taataatcag ataggtttag atttaggaat taaagaattt
     1021 tgtatctcaa gttgtggaga atttatagaa aatcctaagt atcttaagaa gtcattaaac
     1081 aaacttgcta aattacaaag agaattatcg agaaaaacaa ttggtagttt aaatagaaat
     1141 aaagcaagat taaaagttgc aagacttcaa gaacatatag ctaatcaaag aaatgatttt
     1201 ctacaaaaac tatctactaa attaataaaa gagaatgata ttatttgcat agaagattta
     1261 caagtaaaga atatgattaa aaatcataaa ctatctcgct caatttctga tgtatcatgg
     1321 tcagagttta ttcgtcaatt agaatacaag gctaattggc atggtagaca aattgtaaag
     1381 gtaggtaaat tctttgcaag ttcacaaata tgcaataaat gtgggtataa aaacgaggaa
     1441 gtgaagaact taaatataag agaatggatt tgtcctagtt gtaatgaaac tcacgataga
     1501 gatataaatg caagtataaa tatattaaaa gaaggattaa gactaataac gattcaaaat
     1561 aaataacgta tgtaagaacc gtaggaacta cggggatagc ctgtggagaa ttagtaagac
     1621 atatgttagt atgcaaaatt ctatgaagca ggaaccctgt gactttagtc atgggaggtt
     1681 cagtttattt caaataatat tttacgtaat ccatctatgt taattggatt aatagcagca
     1741 tttggtcttg tactacagaa gaagaaagca tctgatgtta taaaggggtc actacttgca
     1801 gctttcggaa ttataattat ggagactgga acaggtatgt tagttagttc aatagcacca
     1861 attaatggag ctttccaaag tataagtggt ggtgcagtaa caaaaggttt aagtgatgtt
     1921 acttttactg caagttatgg tggaattata ggtttagcaa tgtttgcggg tttagctatt
     1981 cacctaatga tagcaagatt tactcctatt aagacaatct tcttaacagg tcacatgctt
     2041 tattggttcc cattcatatt tgtagcttca ggagttgaag ctggattaaa tggtggagta
     2101 ttaatagctt ttgcagcagt attatcagca ctctattggt caataatgcc ttggttatta
     2161 agaaaatatg tgtttgatgt tataggagat gattctttta cattaggtca tccatctggt
     2221 tgtttagcac ttatatcagg atttgttgct aaattagtag gaaataaaga aaaatctaca
     2281 gaagatataa acatacctca aagtttatca ttttttagag aaatatctat aagtggtgga
     2341 atggcagtgt ttttaatgtt tatagttgtt gggatagtaa taccaggtgc gtttaaagat
     2401 agtaatgagt ttattggaac gtatgcaata aagcaaggtc tttcttttgg agcaggtctt
     2461 ataataatgc ttcatggggt aagaatgcta ataaatcaaa taatgccagc atttcaaggt
     2521 atatctgaaa aagttgtgcc aaatgcacta cctgcttttg actgtccaat attatttaat
     2581 tataaaccaa atgctgtatt aataggattc ttagttgcta tgctaacatc tacagctatg
     2641 attttaatat gtaactactt taatgtattt aatttgctac ttataccact tgttataact
     2701 tcattctttg agtgtggagc agcagcagta ataggagaat cacaaggtgg tatacgtgga
     2761 gctataatag gtacagctat agctgcaata tttatggtag ttttagtagg aatatctgct
     2821 gtaatttact caggaactat tcaaaattgg atactaatat ttggaggtaa cgacctttca
     2881 ttatttggta taattagtaa gtttataggt tcactatttg gaggaatatt atagatatga
     2941 aatttaaata catagatgag ataagaagat taattgatat aatagaaaac gaagaaaaag
     3001 tcaatatgga aaaagcagta caaatccttg ttgaagcaat agaaagtaaa aatgctatat
     3061 tttcatttgg agcttcacat gcaggaatat taacagaaga attattctat agagcaggtg
     3121 gctgtgttct tataaatcca gtatttgcaa aatctctaat gttagataca gcgcctataa
     3181 ctcacacaag taagatggaa agattagaag gatatggtac agctctagct gataaaactc
     3241 caattaaaaa aggagatgta gttatagttc attcagtatc tggtagaaat ccagtaagta
     3301 tagagtttgc tatgaaagct aaggaaaata aggcaatagt aatatgtata actaatttaa
     3361 gttactcaaa aagtgtatct tcaagacatt cttcaggaaa aaaactttat gaagtatgcg
     3421 atttagtgat agataatcat ggtcaaaagg gagatgcctg tgtagagata gatggattaa
     3481 accaaaaagt tagtccaact tctactgtaa tagcaactac tgtgatgaac tctattatag
     3541 ctcaagctac tcaggaatta gttaataaag ggctaaagaa tcctccgatt ttctatagtg
     3601 ccaatattga tggtggagat gagttaaata agaagatttt tgatgaatat aaaagtgtaa
     3661 ttcattatga atattaaggt taaaaaaata tgttaatgaa ctttgaagtt tatcaacaaa
     3721 gaatattaat aatatataat aaatgctatc atttaaaagc tgtaggtaaa aatctacagc
     3781 tttttattat tgtagattaa aaagatgcgt aattaaattt taaattggag aaaataaatt
     3841 ggaaggtatc aattataaat attagaaacg ttgtcataga aaaaaactat attaaaaaaa
     3901 agtacataga aaaaactgat tagacaataa atacatgctt tgataatatt ttaaatggag
     3961 tgaaaatgtg tagaattata atgaaaaaag aaaataaaaa tgaaaaaaga aaataaaatg
     4021 gagggaataa aagtgggtat gttaaaaaaa ttatgttcac tagctatggt agcaattatg
     4081 actacaacac ttataacagg gtgttcaagt ggaaatggaa aagattcaaa aaaggatggc
     4141 gaaaaagaaa aactagttct ttggatgcca ccagtagaaa ataatatgaa ggaagtttgg
     4201 gaccctattt tagataaatt tgaggaaaaa aataactgtg aagtagattt acagataatt
     4261 ccttgggata attattctga aaaatttgca acagctataa gtgctggcga aggtccagat
     4321 gtaggatata tgtatgcaga aatgtatcca cagtttatag aaagtggagc agtagaagat
     4381 ttgactaatt atgcaactaa agaggataaa gagcaatcta tttatataaa aacatctgaa
     4441 atgatgggtg gaatgtatgg tatgccattc caagcagcaa atccaggggt attatactat
     4501 aacaaggata ttttagagaa attaggagaa aaacctccta aaacatggga agattttaaa
     4561 agaatttgta aaaaagcaac acaagatact gatggtgatg gaaagataga ccaatgggga
     4621 ttagctcagg gatggggagc taaaactttt ggtaacttaa actggaactg gtatccttat
     4681 ttatggcaag ctggtggaga tatttttaat gatgatttaa aatcggttaa atttaatgat
     4741 aagagtggtt tagaagcagc taatttctta aaagaattac aagcatatgt gcctgaagat
     4801 tctttatcta aagatagtaa tgaaatgata gaaagtgtat ttggaccagg gaaagcagca
     4861 tttacaataa tgttatcttc tgctgcaaca agtgtatttg atgaatcatt cccggattta
     4921 aattggggat ttgtaactgg actagaagat aagaaagcag caacttttgg agcagtagac
     4981 catttatctt taatgtcatc tgctaaaaat aaagaacttg catacaagtt aatacaacat
     5041 atgttaagtg ttgaatcaat gacagaattt cataaagcaa taccaagagc accaataaca
     5101 aaaggtgaac catatcaagg tgatgaaaga ttcaaagaaa tggtggaaaa tgacaaaaat
     5161 gtatatagac cattagtagt aggacctcat ggagttgaaa tatatgaata tttatggaaa
     5221 gaacttcaaa ctatgatttc aggagataaa actccaaaac aagcgttaga tgatgctgca
     5281 aaatattcaa atgatttatt agcacaataa aagtttaaag ctgtaatatg ttgtaaagta
     5341 ctttacagat taaaattagc attgtattta taaatacaat gctaatattt atattggaga
     5401 ggataaaatg gaaaacttag ttagagagtt tgaagaaaat tcaaaaagaa aaaaaacaaa
     5461 aataaatatt aaaccatata tgtatatatt gccattggga ataattttag tggcgtttta
     5521 tgtgatacca ataataatgt caatatactt tagttttaca aagtataata ttattagtcc
     5581 agctactttt ataggtttag aaaattataa aaaattattt acagatgaaa cattaaaagt
     5641 atctattata aatactatta aatttactgt tgttgtagta ccttgccaaa ctatattatc
     5701 attgattttg gcagtttgga ttacaggaaa aggaaatagt aaaatagctt catttgctaa
     5761 aggtgctata tttataccag tattatcatc tatggttttg ataggtatgg tatggagggc
     5821 tttattgaat ggagaaggtt ctattatata tcaggtatta ggtgcctttg gaatagaatc
     5881 atctaaactt ttaggagata gtaagacagc attacctaca cttatgttta tatctatgtg
     5941 gaaaaatata gggtatttta tggtaattta tatttcagct ataatgaatt taccaaaaca
     6001 ctgctatgaa gtagcgaagg ttgatggggc tacaaaactt caagaattta taaaaataac
     6061 tgtaccttta ttgaaaccaa ctacaataat ggttgtattt ttagggtcga tatggtcatt
     6121 acaagtattt gacttaattt atactgtgac aggtggaggt ccaggtatat ctactatgag
     6181 tatagttatg catgcattta acttaaactt taaaaacttt aattctggat atgcaatgac
     6241 tgtagcaaac gtattatttt tgttaatagc agtagtttcg attttacaaa ataagcttgt
     6301 aagacgagac aattcagatt tttagaaagg taggtataat atgaaaacag caagaagatt
     6361 attaagtgat ggtttacttt tatttatagc gtgtgcaagt ttggtacctt ttatatacat
     6421 gttgataata tcattgaaga taacatataa ctcatatagt ttggatatat ctttttcaac
     6481 agtaaccctt caaaactata tagatatttt tactaaaaaa ggatttgcac aatatttttt
     6541 taatacagca atagtatctt tttcaggtgt attattaaat ttagtattta gtacattagc
     6601 aggatattct tttgcgaaga tggattttaa aggtagtgat aagttatttt tatttatgat
     6661 aatgacttta attatacctt cccaagttac aatgatacca ctatatataa taatgaaaca
     6721 cttaggatgg ataaatagtt atcttgcact tattatgcca ataccaactg catttggcgt
     6781 atttataatg agacaagcta ttttaggagt accaaaagag ttattagaat cagcaaagat
     6841 agatggttgc tcggatttta gaattttaat acaaatagtg ttgcctttaa ttaaaccagc
     6901 attaattaca ctagcaatat ttacttttat gggagcatgg aacgagttta tgtggcctct
     6961 tatagctaca actaaagatg caatgagaac attaacagtt ggattatcta ctcttaagac
     7021 attccagata actaattatg gacagatgat ggcaggggct acaataacat ttttacctcc
     7081 atttattttc tatttgattt tacagagtaa atttgttgaa ggtgtgtctt tgtctggtat
     7141 aaaaggttaa atagtctaat ttaataatat ataaagaaac ttatataaat atggaggaat
     7201 ttaaactatg gaaatttcat atgtactgaa agattgcaca ttcaaacaca ataaatactc
     7261 agttgtagaa gctgaaactt ggaaagataa ggatttggat attgtagtag ctaaaggtga
     7321 aaaatttgca tttcaaatta tgttaaaagc aacagaagaa tttaattgta caattgataa
     7381 aagcagtgct atatcatgga aaggcctggg aaatagagta aggttagcat taaatatacc
     7441 taatagttta gaaaataatt ttagtataaa tatattagga tatgttcaag atgatacaaa
     7501 ggcatttgtg aatgatgcta ttttaaggga taaagatgtg ttagtagagc aatatttgcc
     7561 tcaaacattt tggattgaag gtcaagtacc agaagatttt gaagaaaata accttgatat
     7621 aggtattgat atatttaaaa gctttggata tgaagatgaa gaaaaagttt gtacaattga
     7681 tgttcctgta aaagtaagaa atgttgtgct aaagccttta gatgaaagca agttcttttt
     7741 agatttatgg caacatccaa gctgtttagc tagaatgtat aaagttgagt tatggtctga
     7801 tatacatttt gaaatagtcg ataactattt aaaagaatta gcatctttag gagaaaaagt
     7861 tgcaacagtt atagtttcag attatccatg ggcagggcaa agttgttata aagtttataa
     7921 aaatccatca aacttatatg aatataatat ggtaagtgta tcaaaaggat tggatggaaa
     7981 gattaaatgt aactttgaga gtatggatag gtatataagc atagctgata agtataaaat
     8041 ggctaaggag atagatttat ttggtctatt aggtaattgg tgtgctggtg agtttggaaa
     8101 tcctgtagaa ggttataagg accctataag agtaagatat tttgatgaaa aggataaagt
     8161 atttaaattt ataaacaata caaatgattt aaaagaatat ataggattag tattaaatca
     8221 tttaatagag tgtggattat gggacagagt aaggattata gctgatgaac caaataatcc
     8281 agaagtagtc aaagagtgta tagaatttat aaattctact gtaggtactc atcaagttaa
     8341 atataaatca gcaactcacg accaaaactt tttagataga gctaaagatg aaattgatga
     8401 tatgtcaata aacttaaaac ttactataca aaattataaa gatatagaaa gtcttaagaa
     8461 aaaaatcaat gacaagggtg ggatattgac ttggtttgta tgctgttttc cagaaaaacc
     8521 aaatagtttt ttaagttctc cttttgtaga aaatagaata ataggatggt atacttatta
     8581 ctttggtctt gatggatttt taagatggga ctataattta tggacagaag acccttggaa
     8641 agattcaagt tacaaattcc ctatttggaa agctggagat atgttctttg tttatcctgg
     8701 aaaagattta aagccagtta gaagtgttag aatggagaat ttaagatttg gaatacagga
     8761 ctttgagtta tttacaatgt tagaaaaaga aaagggaaga gagtatatag aagttgagct
     8821 tatgcaagaa ttattaaata aaaaagaaaa tgctgaaatt aaaggttttg gtgacataga
     8881 actaggatat tcacttgata attggggata tgataaagtt aaaaaatatg tattagagtt
     8941 gttagacaga gtataaaaaa tataagagtg tctcaaaatg aactttttag ttcatgaggc
     9001 actctttttg tatgaaatca atatagtttc attgtttcaa caatgaaaaa taggattatt
     9061 tctattctaa gacagccctt tagtttttta gttgtattca atgttttaaa actaactata
     9121 aacgctataa agtttataaa tgacaataat tatatgtaac atgatgtact ttaatttgct
     9181 ataataggga ttgaataata ttatatggaa gataacattg tttgggggga ttaagataaa
     9241 aagaataggt attatcataa tgtttttttt aagtatagct tttattggat tttatcttgt
     9301 aataagagat aacagtgata cagttaagat gcaacgaggg ataaatatag gtaatgcact
     9361 agaatcacct aaagattttc cgtgggatgt aaaaatgtca aataagtttt ttgatgatat
     9421 aaaagatgca ggttttgaca cagtacgtat accagtaagg ttttctgatt atacgtcaga
     9481 ttctaataat tttaaaattg atgaagaatt ttttaaaaag attgataaat atgtagatta
     9541 tgcattggat aaagacttaa tagttgtgtt ggatttacac cattttgaag aaattatgaa
     9601 agaaccaaga gttcataaag aaaagttttt aaaaatatgg caacagatag ctaataggta
     9661 tcaaaaatat gataaaaaat tggtatttga attattaaat gaacctaaag aaaatttatc
     9721 ttcacaatta ctaaacgaat acattgaaga agcaataaag ataattagaa aaactaaccc
     9781 caaaagaact attatagtag gaccatataa tttctatcaa attgattatt tgaatgaatt
     9841 aaatattcct aaagattcta atatagtagt ttcttttcac tattacgagc caaatgattt
     9901 tgcatttcaa ggaaatatct atcataaggg atttgagcac ttaagcaata ttacttggga
     9961 aggaacaaat gaacaaatgg attatttaaa aaaaaggttt gatactgtgg aaaattgggc
    10021 aaataaaaat aaggtaaaaa tcttcttggg tgaatttgga ataacaaaag aggcaccaga
    10081 agcttcaaga agagcatgga taaaagctgt aagagaagaa gctgaaaaac gaaacttttc
    10141 atgggcttat tgggagcttg catctggatt tgggatatac aatcaaattg aagggacttg
    10201 ggatagagat atattaagtg cattaataga aaagaggtga ttttatgaag aaaaattcaa
    10261 aaaagatact tataacttta actattatta ctaatgtcat atatatattg tggagaattt
    10321 tttacacagt acctaaagaa gaagggaagt ttgccttaat atgtgccata atacttcttt
    10381 ttgtagagat aatgggtatg atggaaatgt ttgtgcatta ttatggtatg tcaaatattg
    10441 agtatcctga aaaaccaatc ataagtgaag aactttatcc acatgtagat gtatttattg
    10501 caacatataa tgaatctgtt gatttggtta gaaaaactgt aaatggatgt attcatatgc
    10561 agtatccaga taagaaaaag gtacatatat acatatgtga tgatggaaat cgtaaagaaa
    10621 tgcgtattct agcagaaaaa atgggtgtaa attacataac tagaactgaa agagaaggag
    10681 caaaagctgg aatttaaata atgcaatgca acatacaaat tcccctttga ttgctacatt
    10741 tgatgcagat atgataccaa tgcatgactt tttaattgct acagtaccat actttttgaa
    10801 aaatgagcaa gctaaaaaag atggagaaaa agaggagtat gaaaaggttg gttttgtgca
    10861 aactccacag agtttttata atccagattt gttccaattt aatctgcact ctgaaggaag
    10921 gattccaaat gagcaagatt atttttacag agatattcaa ttggctagaa ataggacaaa
    10981 ttctgtaatt tatggtggta gtaatacagt tatttcaagg gaagcacttg aagaagttga
    11041 tggattttat acttattcta ttacagaaga ttttgcaaca ggtatcttaa ttcaaagtaa
    11101 ggggtataga tgttatgcta ttccagaagt acatgcatca ggtttatctc ctacagattt
    11161 aaaaagtctt attaagcaac gtgaaagatg ggcacgtgga tgtatacaaa ctggtagacg
    11221 tctaaatatt ttgtttagga gaggtttagg tttttggcag aaaataagtt atatttcttc
    11281 aattacttat tggtatgcaa gtataaaacg ctttgtatat ataatggcac ctattttatt
    11341 ttccgtgttt aatgtgattg tagtaaaatg tacattactt caagtattgg tattttggtt
    11401 gccaatgtat attttaagta gtttatctct taaaatattt tctcaaaata taagaaatac
    11461 tagatggacc aatatatatg aaactataat gtttcagtct ttaatgccag cagtaatact
    11521 tgaaacgttt gcaatatcta aaaataaatt ttcagtaaca aataagagta aattagaaga
    11581 aaatagaatg tataagtttt tacagggaat accttatttt atttacatgg tcttatctat
    11641 cataggaatt ttaaaaatgt ttgtagcaat atttaaaatg agttcaatga catattcagt
    11701 tgtattattt tggttgattg gaaacctttt taaccttgtt atggctacat tatttatatc
    11761 agggagacag cagttaagaa agtctgagcg ttatattgct gaaattgatt ttaagttaaa
    11821 gcaaaattct tatgttcttt cttcaaaaac aattgatata tcagaaaatg ggtttgcatt
    11881 tttgctagaa aatccagaat atatttcccc agaagaagag tttgaggtag agtttagaga
    11941 aaaatctgga aatgaaatgt atatagctaa tatgaaagca aagattgtta atgttgtgga
    12001 agtaaattca aagtggaaat atgcagctta tattacacat attgaagata gtgaaataga
    12061 taattggatg tgtattgttc acgatagaat acctacttta ccaatgacaa tatctaatca
    12121 actaggtttc tttgatgatt tgcaaattaa tgtaaaaaaa cgtattgaaa aaactagaac
    12181 attatcacga agaagtccaa gaatcaatat gaattttcag atggatataa aaaatacagg
    12241 taaattaaga atagtgaatt ttaattatca atatgtatta ttaaattttg agaataaaag
    12301 tatttatcct aaagaaatag cattggaaat taatgaaaat attgtgttag aatgtgattt
    12361 gtgtgagggt aaaattgatg aaagaggtat cttatataaa gtaaataaca ttgacagcat
    12421 aatgcaaaat ttctttttaa gagatgaaat gatggattgg atattacaaa ataagacaat
    12481 tcttgtttca aagcctagtg aaaagaagga aaaaagtata gatgagtttg aacccatgga
    12541 atatatttag gagagatgaa atgaagactt taaaagtgtt tttttgttct acaaaatatc
    12601 ttattttggt gtttattttt tttataaata ctacttttat aagttcagca acaagtgata
    12661 aaattttaga tttatccttt aaaaagatag agactgactt aaattctaag attacatatg
    12721 aagatacagg tgtaaaaata gaaacggata gttctaaaag cgataaggaa cgttatttat
    12781 atatttacca aaatataaaa gaaaattgga gcatgtataa taatttttat attgaaattc
    12841 aaaataaaaa taaaagttct cagagaataa atttaagcat acaaagtaaa aatatgcttg
    12901 aatttagatt aaaagaaggt agtgaagtat ttttagaaga taaaaatata atatattcag
    12961 ataaaataaa agaaggatgc atagaagtac caggtgaatt tgaaggcaaa atttatgtga
    13021 actttaatag ccttataaat gaggaaagta atgttgtgct ggactcaagt atgctttcaa
    13081 acattgttag ttggggaatt acgtttattc catctgatga agaacataat attgttatta
    13141 taaaaaagat atctttattg tctgaagaga aattaagatt tttaaataat attaaaataa
    13201 ttggcgatga agaagtacaa ataccagtat tgggtcaaag tataagtcag tatgaggtat
    13261 taggtcttaa aagtgattct aaaattaagt actctcttat gggaaaacaa gataatgtga
    13321 gtatatctca aaacggtaaa ttaacattaa gtaataaagc caaacctgga caaattattt
    13381 tacaagttaa tgttgatgat aagtttaaaa ttggaaagaa aataacatta actgaatctt
    13441 ggagtattaa taaaaaagat aaggatggtg ttccatatac attagtatct cctgagcaat
    13501 caccaacagt acaagatatg aaaaaaataa attttatgaa taatatcata acgtttgttc
    13561 gaattttatt tgtatctcta gtaataatat gctttggaat atatttatat tggaaaaaat
    13621 gttcaaaaac taagtaaaga gaggaggtta gattgtgtta ttttcaagtt taattttttt
    13681 attttatttt ttacctataa ctttggtttt atattatgta tttaggttta accgaacgat
    13741 acagaacatg atattgcttg ctgttagttt atttttttat gcttggggag agccaaaatt
    13801 tgttgttatc atgatagttt ccataataat gaactatata tttggtttac tggtagatag
    13861 atatagggaa agtaaaataa aagtaaaagt ttttttagtt ttgatgtgtg catacaatat
    13921 aggtgtttta tttatattta aatatctagc ttttgcgcta agaaatatta gtgaacttat
    13981 tagtactgaa ttgactatac ctagtattgt tttacccata ggaatttcat tttttacatt
    14041 tcagggaatg tcttatgtta tagatgtata tcgtagacat ggagaagtcc aaaagaaccc
    14101 tttttatgtg ggattgtata tagcattttt cccacaatta atagcaggtc ctatagttag
    14161 atatgagagt gtggcagaac aaattctcaa tagaaaagaa acttggaata aattttctat
    14221 tgggacttgt aggtttataa caggtctagg taaaaaagta ttaatttcaa ataacatggc
    14281 tatagtagca gactatattt acactatgaa tagtcaagga gaaatagctg catcacttgc
    14341 atggcttggt tcaatagcct atactctgca aatattcttt gatttttctg cttattcaga
    14401 tatggctata ggcttaggtc ttatgtttgg ttttaaattt gaagagaact ttaattatcc
    14461 atatatatca aaatcaatta gtgagttttg gagaagatgg cacatttctt tgggaatgtg
    14521 gtttaagagc tatatatact ttccgcttgg aggttctaga gtagcaaata aagatattat
    14581 gattagaaat atgtttatag tgtggttatt cactggaata tggcatggag cagaatggac
    14641 atttgtaatt tgggggattt taaactttgt tttcctaatt atagagcgat ttataatgtt
    14701 tgaaaagatt gaaaatcaca attttataaa acatatttat gctttattag tagttaattt
    14761 tggatgggta ttgtttaggt cacctaattt aaaagaagca tataattatt ttaaagctat
    14821 gtttggaggc aatggagcta tatggagtga ttatacatat atgtttttaa aagaatactg
    14881 ggttttcttt atatttgcat ttatttttag tgtacctatt gcaaagaaaa tcaataagtt
    14941 tgttgttgaa caagctaaat atagtatgat atttaacgta ttttatccaa tctcagctat
    15001 tttattgttt tttatttctg tgacatattt agtaactgga agctataatc catttattta
    15061 ttttaatttt tagttgggag gaaaagtatg aatagatttt ttaaagtaag attaataact
    15121 gcaattatgt tcttaattat tttattcgct ttctctatct ttaatgtttg ttattctgga
    15181 aaagatacga tacaggaaat aaaaaattca atagctgaaa aaagtagttt aaaagataca
    15241 atatcatcta ttgataatac agtgaatgac aaagtaatat acaaaaatac gttcattgaa
    15301 acttatggct atttacaaaa attaatgggt aaaaatgagt tttcaaaatt tagtgttgta
    15361 aaagatactt ctggaaagat gcattataca tattttgcta aagctccaaa tccagttggt
    15421 gtgatagcag atagggtaag aaaattttct gatgaaatgg aaaagcaaaa cactaaattg
    15481 gtttacctaa tgactccaga taagtatata aaaggagtga caaaatttcc aaaaggaata
    15541 ccttattctt atagtaatga gacagcagat aattttttaa atcaactaaa aaagtataat
    15601 gtggactata ttgattttag agaaaatatt ttaaagagtg gtattcccaa agatgattta
    15661 ttttttaaaa cagaccatca ctggaaagta gaaacttctt tttgggcatt tggagaatta
    15721 gttgagcaac ttaataaaaa atacaatatg aatttagatg aaaatcatta ttatagagat
    15781 aaggacaatt ataattcaat tgtatatcct aagtcatttc ttggttctat gggaagaaag
    15841 actggtatct tatatggtgg tatagatgat tttacattaa tttatcctaa atttaagact
    15901 aattatactt attatacaga ttctaaatct caaaaatttg aattaaaagg aaggtttgaa
    15961 gagagtttaa tattatcata tccttttaat gcagaccttg atttaatgga tggtcaatca
    16021 gataagtatt ttacatattt attggggaat agaccattag ttaaaataaa aaatatagaa
    16081 aatccagatg gattaaaagt gctgtttgtt aaagattctc taattgtacc aacagcagca
    16141 tttttttcct ctgtttgttc atctatagat atgatagacc ctagatatta tgatggagat
    16201 atactagaat atgcaaaatc ccatgactat gactttgttt ttatatctgt atatccccaa
    16261 aatcttacaa aagaattttt tccgttttgt gagtaaattt ctaaaaaagt tttctgataa
    16321 aataaatggc taactggtaa aaagttagcc atttattttg agtgttattc taagaaatat
    16381 tttttttact aaatacttct ttatctattt ttccaagtga ttttcctaca gtaattgatg
    16441 ataccatagc accattaaca tttatcatag tacgtcccat atctagtata gggtctattg
    16501 caagtatacc accaactaat ggaaaataag aaccaagacc aactccagag ataacaactg
    16561 aaacggccat tgtagcagtt cctggaagac cagctattcc aaacgaacta attgttataa
    16621 caattaacaa cataacataa aagctcatat ccatgtttac tccagccata ttggcaagtg
    16681 ttacagccat taaagcagga tatatacctg cacaaccatt catacccata tttgcaccta
    16741 aacttgctgt aaagcttgat acaccctcat caactccgtg attttgtact agactttcta
    16801 tcgttacagg caatgttcct aaactagaac gtgaagtaaa tgccagaagt aaagcttcag
    16861 ttgaattttt tatataagta atgggattta gaccatttaa agttataaaa attaaatgta
    16921 taataaataa tattgcaact gatatataaa gagctaatat aaactgtaaa acagatacta
    16981 ttgaactaat accttgtgaa gttattgtat ttgcaagcag agcaaccaca gcataaggca
    17041 taaatttaat aacagtcata gacacactta caattatttt gtaagatgct tctataaaat
    17101 ctataaatgg ttttattgtt tcaaaatgtt ttttacttaa tctctttatt gaggtagcta
    17161 taaatgtagc aaatattatt gtagctacaa tatttccatt tgccatagcc attacaggat
    17221 tagatggtag taatcctctt aaagtatcca caagagagtt tatttcttta agttcttgat
    17281 tttcaactaa agtaggcata ttagctccta cacctaactt cattatattt gcaacgataa
    17341 ttccaacaat tgcagctata gcagtagtag caagaagcat acctaaagat ttaaatgtca
    17401 gagttccaag attgttatct ttcatattta taattacttt tataatggat aagaaaacta
    17461 aaggtactac taacattttt aataagtcca taaatccaga accaaatagg ctataccact
    17521 tacttacttc ttgtagccaa gtgacttgag ttgtatcatt aggaaaacca gcaaaagctt
    17581 gtattatgat tcccaatatg agtcctagta ctgtagcata tattgttctc aatgaaaatt
    17641 ttattttttt cttttcaaat tgtctaacaa taaagaatgt acctagaagt aatattatga
    17701 aaaaaattgt tttaaaatct gtaatcataa gaaattgtga taaaaatgaa ctttccaata
    17761 tttttctcct tttatttaaa aataaatttt tctaagttta aatgatatca atatattaaa
    17821 aaaatgtcaa tattatatca caatttactt aaatagagta aaacaagttg attttgaaaa
    17881 tgtgattaag tatagaaaaa tagggtataa taaatgttta aagaatttga attgtggtaa
    17941 gaaaataaaa aatagctttt ataagatttg atttcaaatt aaaatatgtt aaataaataa
    18001 ctaaaaatta ttataaaaaa tgttttcata gaatatatat caaatgaaaa gcttaaaata
    18061 agtgaagaaa ttaactagac atcaaaggag agataaaatg agagtaataa taattggtgg
    18121 tgttgcagct ggtatgagtg ctgcagcaaa attaaaaaga ataaaacctg agtatgaagt
    18181 tgttgtttat gaaaaaactg aaatagtatc ttttggtgct tgtggattac catactttgt
    18241 tggtggattt tttaatgatg ctgatgaatt attagcaaga acaccagaga aacttagaga
    18301 agctggaatt gatttaaata tatttagaga agtcgttgag gttgatagtg aatctaagaa
    18361 aataaaagta aaaaacataa aaacagatga aatatatgaa gattactatg ataaattgat
    18421 gatagcaact ggagcaagaa gtattatgcc tcctataaaa aacataaaat tgaagaatgt
    18481 ttctacatta aaaagtttat atgatggaga atatctaaaa aaacttttaa gtaatgaaga
    18541 taataaaaga gtgacaataa taggagctgg ttttataggg ttagaagcag ttgaggcatg
    18601 taagaaatta ggtaaagatg tagatgtaat tcagttagaa gatagaatat tgccacaagt
    18661 atttgataag gagatgacgg atgtattaga agaagaaata aatagacata atgttaattt
    18721 gcacttagat gaaatggttg tagaactttg tggagaagat aaagtagaaa aagttataac
    18781 aaacaaaggg gaaatagata cagatgtagt aataattgct actggcgtga gacctaatac
    18841 cgagtttttg tcaaatacta atataaaaat gctaaaaaat ggagctatag tagttgatga
    18901 gtatggaaga acttctgtag aagatatata ctctgctgga gattgtgcaa ctataaagaa
    18961 tatagtaagt aatgaaaatg tatatgttcc tttagctact ggagctaata aattgggtag
    19021 gatagtagga gaaaacctag caggaagaga agtatcttat caaggttcac tatcttcaag
    19081 ctgtataaaa attatggata tggaggcagc atcaactgga attacagaaa agcaagctaa
    19141 agatttagga ataaatgtca aatctaaatt tatttctgat tataatcaaa ctcattatta
    19201 tccaggaaga aataaaatat atgtaaagct tatttatgat gcagatacaa aagttatttt
    19261 aggcggacaa gtggctgggt ttaaggatgc tgtccaaaga gcaaatgtat tggcagctgc
    19321 tatctttgga aaaatgacca caagtcaact tggaatgtta gatttatgtt atgctccacc
    19381 atttgcaaga acatgggatg ttttaaatgt agctggaaat gtatctaaat aatatttaga
    19441 ttatagctaa tatagttgac aagcaattaa gggaatgaat ttacctaaac tatttttatc
    19501 atggtaaaat tcattcctaa atttttattc caaattttta gaaataagga ttccattaaa
    19561 ttattcatca aaatttaaac gcaagcttaa tctatcttct ccaagaatag tattttcaaa
    19621 aacattagta gcattttcta aatacatact aggtatgtct aaagaaggac taaaatgagt
    19681 cagtaaaagt tgtttgacat ttccaagttt tgcaagatta gcagcttctc taaaagtcat
    19741 atgtttgttt ctaacagctt ttgatatatc taaatcatca ccatacatgg cttcacatat
    19801 aaataaatca ctatctttta tgaaatctgg tattgattct ataggtcttg tatcagttgt
    19861 aagacttatc ttaactccct ttctcaattc tcctaagacc atatctggag tgtattgttt
    19921 tgaatctaaa actatatttt gaccttcttg gagtttgttc cataatattt taggaacttc
    19981 gtttttagtt gccttatcta tatcaaattt agggtttctt ctaaaattaa aactgtatcc
    20041 taaacactct atagagtgtt ctaaactgat tgttgaaatt tctaaatttc ttaaaacttt
    20101 attatctaaa gaaaaattgc cttgagggtt ttctataatt tttaaagtgt atggtacata
    20161 ttctactaaa tttctcatag aacgtataca atcgactatt ccaactggac caactatagt
    20221 taaatcactt gtacgaccac tattcccaat tgttgataaa agtcctaaaa gtccaaatat
    20281 atggtcacca tgtaaatggg ttatacaaat caagtctata tctttaaagc cacatttttt
    20341 tagcttcata gatacctgag taccttcacc acagtcaatt agtatttttc tacctttata
    20401 gtttataaat acagaggata gaaatctgtt tggcataggt acatttccac cacatccaag
    20461 taaagttaaa tctatcattt tttccccttt ctaaaattaa cacaaatctg ttgtttgttt
    20521 ttattatgat atcctattat aattatttta acattaaata taacaataaa tgtgtatttg
    20581 agtcaagaaa tattgtcaaa taaagttttt atatagcaat gtataaaaat ttggaaaacg
    20641 aaaagtgaaa attatattta ggagtattta aaggaggaaa atttaatgaa agtattaaag
    20701 aaaattacta ttttgtcatt gattacatta actttagttg gttgtagtaa cggagaagag
    20761 aaaaaagtgg ttgaaggttc taagaaaact caaaattcta aaaaagatga acagactata
    20821 atagtgcata aagatgcaaa tatattgaaa gataggtatg agccaccaaa aggatttaaa
    20881 agagttgatg tagaaaaagg cagttttgaa tcatttctta ggaatactaa gctaaaaaat
    20941 tatggagaga aagtaaagta ttatgatgga agaactaagg aatcaaaagg tatttatgat
    21001 agtgtatttg atgttgatat tggcaataga gatttacatc aatgtgcaga tgcaattatg
    21061 cttatgagag cagaatactt gtatcagaat aaactatatg atgaaataag ttttgatttt
    21121 gttgatggtt ttaaggcgaa gtattcaaaa tgggcacaag gatatagaat ttcagtaaaa
    21181 gatagtggtt caagctggta taaagcaact gaatattcaa catcttatga gagttttaga
    21241 aagtttatgg atatagtgtt tgcatattca ggaacgttat ctcttgaaaa agaattggag
    21301 cctgtaaaag ttgaggatat gaaaatagga gatgtgttta tagttggtgg aagtccagga
    21361 catgcaagta taattatgga tatggcagaa aatgaaaaga ctggtgaaaa ggtatttatg
    21421 atagcccaaa gctatatgcc tgcacaacaa acgcaacttc ttattaatcg taaaaatcca
    21481 aatttgagtc cttggtatag tttagatttt gaaggtgatt tgacaatacc tcaatggact
    21541 tttaaaagag accagttgaa aagattttaa aataataatt ttatataata aaagaaaaat
    21601 atagatttga aaaaatgtaa ttaatttgaa aaaatgtaat tagattaaaa ctaattggat
    21661 atggacaaaa ttaaattgat agataaatat taaaaattac actaattaat cttactatag
    21721 tttaatattt atctaaaaag ttttatgtaa aatataaatt ttatattaaa gctaaaacta
    21781 atttgaggag gctaatttat attgaagatt agaaggtatt taattttggt aattataatt
    21841 ctaattacaa caagtcagag ttttatttct tatgggctag agcgatttga agaaataacc
    21901 atttttcaca ctaatgatat acatggaaga tatgctgaag gtgatgacca tatacaaata
    21961 ggaaatctag ccacattgaa aaaagaaact ccaaactcaa ttttagtaga tgcaggagat
    22021 tgtttgcatg gacttccaat agtaaatatg gatagaggga aaagtgcaat tgaattaatt
    22081 aaatgtgctg gatatgacta tattactcct ggaaatcatg actttaatta tggaaaagat
    22141 agactgtttg agttatctaa aagtgctaat ttaggagaga atactttaaa atttttatca
    22201 tcaaatattt ttgaaaatgg aaaaaaagtt tttgatagta atgatataaa agaaatagat
    22261 ggaataaaaa taggattttt tggactctca acccaggaaa caaagagcaa aactggattt
    22321 aaaaatgtgg aaggattaga gtttaaaaat cctatagaaa gtgcaacaga acaagtaaat
    22381 gaattaaaag gaaagggtgc caatgtaata gttgcaatat gtcatattgg tacaaatagt
    22441 ttaagtaagc caaatagtat agatatagca aatgaggttc aaggaattga tttgattatt
    22501 gatggacaca gccacactaa gtttgaaaat ggtaaacaag tgggtgatac attgattgta
    22561 agtgcaggac aatatttaga aaatattggc gagataaaac taactttgga tactaatgat
    22621 gtagaaaata taaaaataga aaataaatca gttagattaa ttggaaaaga agaagcatta
    22681 aagtataagc ttgactccgc tgtaagtaaa aaggttgatg aaataaaaga acaacaagaa
    22741 aaaatattag ataaagttat aggggtaact acaaatactc tagatggttc atatgaaaat
    22801 gtaaggacaa aagaaactaa tttgggtaat ttaataagtg acattttact agataaaact
    22861 aaggctgata tttctttatt taatggaggg aatattcgag atacgataga aaaaggcaat
    22921 ataacaagaa gagatattgt agatgtattt ccttttaaca atactatagt taccaaagaa
    22981 cttacaggag cacagattaa agatgtattg gagcatggag taaaacttta cccagaaaaa
    23041 agttcagcct ttttgcaagt gggaggaatt tcatattact ttaatcctaa acaaaaagaa
    23101 ggcgaaaaaa taacaaatat tcaaaaagaa ggaaagccat tagatttaaa taaaaagtat
    23161 gtagttgcaa cgaatgatta tatagcatca ggtggagatg aatttccttg tttttcaaaa
    23221 gagccaatac taaaggaatt tggaaattta gagagtgcag tcattgaata tatagagtac
    23281 aaaaaagaaa tatcaaaaaa tgtagatgga agaattggta ttaaatctaa agatgagtta
    23341 ataagcaaga atcatgagag tgatttgata ttaagtgaaa atgaaaaaaa tatcaatgat
    23401 aatgaagaga atctatctaa taatataaaa agctctgata gtgataaaaa taaaagctta
    23461 gataaaacaa gtgctagaga aagttctaat ctaggaatat ctaaaagaga agctaaagaa
    23521 aaatctccta aaacaggaga tttaggtttt tcaaatagta taattatatt tatattttca
    23581 agtacattga tatgtttatt gaattttaat caaaaagagt taaaggataa aaagtcaaaa
    23641 taaagattaa tgagcaaaaa ttagtagata gtaatttaat tttagataat agtaaacagc
    23701 aaaggaaatt agttatataa atactaattt cctttgtttt atgttttaac atgattactt
    23761 ttaaataaaa ttgtatttta tgaaaaataa ttacactaaa tggcatagta ttatggggat
    23821 ttttagtttt tattcaagta aatgaaatca tattaacaac ttctgatata tttacaaatt
    23881 gataaaaaaa tgattttatt agtatgatat aatttagcta gtatgtatga taatggagga
    23941 tatagggatg aataatatat tacttttgga agatgacaaa tctttaaata gaggaattag
    24001 ttttaaatta aaaaaagaag gatacaatgt ttttagtgca tttggtatag aagaagcaaa
    24061 aaatattttc gcaaaagaag agatatgctt aattatttcc gatataggtc tacctgatgg
    24121 tagtggtttt gacttttgtg aggagattag aaagaaaagt gatgtttaca taataatgtt
    24181 gacagctctt gatgaagagg tggatattgt cacaggatat gatttaggtg cagatgatta
    24241 tatcacaaag ccttttagct tgatggtatt aatatcaaaa gtgaatgcgc ttatgaaaag
    24301 agtaaataca gtaaaaaatt atactctatt agtttgtgat gatttattct tttattatat
    24361 agaaaataaa ttaattataa gaacagatga taaggaagaa gagataattt taagtaagac
    24421 agagacaaaa ctattaaaat atttgatgga aaattctatg cagacattga ctaaagagca
    24481 actgttagaa tctttatggg atagtagtgg taattttgta gatgataata cgattgcagt
    24541 aaatataaga agacttagac aaaaggttga aaaaaatcca tctgaaccta aatatataaa
    24601 aacagtaaga ggggttggat atatgtgggg agaaaggagt ataaaaaaat gctaaaaaat
    24661 atgaacctca aagatggttt aagtgtgaaa gatgattata taatgagaag agttattgta
    24721 tattctgttt tatctgtact aatagtaacc cttgtattta tactttttga atatagcaga
    24781 ttacataaat tgtatgaaca tcaaataaaa aatactcaaa atattgctgg aatattgatt
    24841 gaaaaatatc ctaatgatga aatagatata gtaaatccta tttataattc tgattatagt
    24901 aaggtgtcta ctggagaaaa ggcatttaag aaatttggct atggattgga aaataaaatg
    24961 tcaaatgata aaaattttag cttatactta aaatcttttt ttaaggaatc attttttatt
    25021 tttttaatga tgtttatagt agttataggt ataatatatt attttataag atacataaat
    25081 aaaagattga gtaaaattta ttttatagta gataagatgg cacaagaaaa ctacttgaag
    25141 gacactactg agtcagaaga aactggttat ctagtagaat ataatgagta ttttaaagaa
    25201 ggtacattta gtaagataaa taactgtcta tatgagctta atagaagttt aaagataaag
    25261 tttattaaat tagaaaaaga aaaagaaagt gtaaaatcat tagtaacaga tatttcccat
    25321 caattaaaaa ctcctttagc atccttaaaa ttatacaata cattacttat agaagaagaa
    25381 ttagaagaag atgaagccaa cgagttctta ttgaccaata aggcttctat aaataaatta
    25441 gaaaatttaa taaattcttt agtgaacata tcaaggttag aaattagcat gataaacata
    25501 aaaaaagaag ataatgatat aaaaaatact attctaaatg cagtaaaaag tgttacccct
    25561 aaggctaaga gcaaaagtat aacattaaat ttaaatgaat ttaacagtag aattatacca
    25621 catgataaaa agtggactga ggagagtatt tttaatgtat tagacaatgc tgtaaagtat
    25681 acacaggtaa atggagaaat aaatatatct gtagaagaaa ctgctaattt ctttaaaatt
    25741 ataatagagg ataatggcat agggattgaa aaaagtgagt ttaataatat atttaaacgt
    25801 ttttatagag gttctgctga tgaaattgaa tctgtagaag gtagtggggt aggattgtat
    25861 ttaagtagaa agattttaga agaacaaggt ggaaatatta tagtttcttc aaaagtaggg
    25921 atagggagca agttttcttt gtttcttaca actatgtaat attaaatgaa aggaaaatgt
    25981 aagcctctac atttattata taagtataaa cagctccaga aataaaaaat taataaaata
    26041 tggggtaata taacttagaa tgagaggata attttatgaa aatactatat actgaaaatt
    26101 tatcaaagca ttatggaaaa ggagaaagct tagtaagagc tttggacaat gttgacttag
    26161 aaataaatga aggtgaattt gttgcaataa taggaaaatc aggttctgga aaaagtacac
    26221 ttttgcatat gattggaggt cttgatatac ctacatctgg gaaggtgtat atagataata
    26281 aaaatatatt tacattaaaa gaagaagaac ttgctgtatt tagaagaaga aaaatagggt
    26341 ttatatttca aagctataat ttaattccat ctcttaatgt atgggaaaat gtagtgttgc
    26401 caatcggttt agatggtaga gaggtagatg aaccttttat aaaagaactt ttaaaatcat
    26461 taggacttga aaataaacac gacgttttac caaatacatt atcaggtggt caacaacaaa
    26521 gggtggcaat agcaagagca ttagctacaa gaccagctat aatacttgct gatgaaccaa
    26581 ctggaaacct agattctaaa acgtctgatg aggttatgag tatattaaaa tctatgtcta
    26641 agaagtacag tcaaactcta gttatgataa ctcatgatga cagtatagcc caaatggctg
    26701 atagagttat attcatagaa gatggcaggg tgtcaaaggt tggtgataaa aatgattaat
    26761 actactaaaa tagcaaaaag taatttaaag caaaataaat caaaaagtat tctaataata
    26821 attactataa tactttcaac cttaatgctt tcttccattg gaatttatat tgttgatgca
    26881 ggagcatatc aaaaagaaaa tactataaag tattcaggta attatcaagg tatattagca
    26941 aatgtagatg aaaaacaggc tgatatattg agtaatcatg cagatgttga attaactggt
    27001 gaaatgaatg gagtaggtgt tgaaaaactg gaagatgatt caaatatttc tctagcatac
    27061 atgaatgaag atgctttgaa attaaatagt tttgaattta tcaaaggaaa attgccatca
    27121 aaagagaatg aaattgtgtt agatagtgga gctttaaagg cacttggtta taaaaataaa
    27181 gatttaggtg aaaagataaa aatatcttat gatgactata aaaatgataa aaaaattgaa
    27241 aaagagttca ttataagtgg aatcttaaaa actagcgaaa tatctgaagc aggtaaatat
    27301 tattatgcta ttatatcaga atcatatatg agaaatacta gagatatgtc acaagaagat
    27361 tttaatattt atgtgaaatt taatgataaa agtaatttat caatagagca agcaaaagaa
    27421 aaattagata aaattgctaa tgatatagga ctggatacta ttaatactgc tgtaaatgaa
    27481 aattatataa atgctttaaa accagatatg gaaacgatta tgggtggagt ttttgtaggg
    27541 ttagtaattg tattatctag tattttagta atatacaata tattttatat ttcaatagtt
    27601 acaaaggttc aagaatttgg aaaacttaga gcaattggtg caactaagaa acaaataaaa
    27661 aatatagtgt ttaaagaagg gttcatatta gccagaatag caataccaat aggaataatt
    27721 ttaggctatg tacttgcaaa tattataata aaatcattta tggatataga tgtaaaatca
    27781 tcacgattac cagttatatt atcggttgtg gtaataagct ttatatctgt tgttttatca
    27841 cttttaaaac caatgaaagt agcatcaaaa gtatccattg ttgacgcagt aagatataca
    27901 ggaaataaaa taagtaataa gagtaagaga aaaggatata aaaatataaa tctaaagaga
    27961 ttatcacatg ccaatttaga aagaaataaa aaacgtactt acatgacatt ggcatcactt
    28021 atacttagtg gaactatatt tataacagta tcaactgcct tagaaagttt tgatgctgag
    28081 aaaatggcaa gagaacactt tccttatgat atagaagtta gactatctgg ttatgaaatg
    28141 aacagtgata aaaatcctaa agataatttg aatattttac aaatggataa tcctctaagt
    28201 aaagattttt ttaatcaaat aaaaaacatg gaaggaatta aaagaataga atctgcaaga
    28261 agtgtaaaaa ttggtatgga ggactatgat gttgaattta agtatgacct tttacaaagt
    28321 ataaatgaaa atgatgtaaa atcattaaac aagaatataa tagatggaaa aataaatctt
    28381 gaaagactgc aaactggaga tgaaatagta attactcatg ttgatacagc aaaagaaatg
    28441 ggagtaaaag caggagataa aataagatta actttatatg atggagataa aaagataaaa
    28501 aaagaattta aagttcaagc aatagctatg ggggttccaa gttttggtat agggaaggat
    28561 tttattgata gaactttgaa atacgattca actagtagct tgggaatata tacagaagaa
    28621 ggaaagtatc aagaaatcaa agatagtatc aagaagattg ctaaaaataa tggattttta
    28681 gagactgatt ttatagatag tagaattgag tcaaataagg ctactatttc atttataaag
    28741 ataatgggat acacgctaac aggcataata ggtgtgattg gatttatgaa tcttgtcaac
    28801 acaatgataa ctagcatagt tactagaaaa aaagaacttg gtatgcttca agctattggt
    28861 cttactaata aacaattagt gaaaatgctt aattcagagg caatgtatta cacttctgga
    28921 atgatgatag gaagtattct atttggaggt atattagggt atattgctgt tatatttcta
    28981 aaaaagactg gattatctta tgcaacatat tctttgccaa tagtaccaat cttgttgatg
    29041 attgtgtgta ttcttatagc tcaatttata acaacttatt tgataggacg aagttttaat
    29101 aaagaatctc ttattgatag agtaagatac agtgaataaa atgcaaatat ttatggttag
    29161 tttaactagc aaaaaacata tgaaaaaata tagttaaaga gattcataat taaataggtt
    29221 aaagcatagt acagagcaaa caatttaagc agatgtttgc tctgtatttt attatgtaat
    29281 caataatata tctaatatac tgatattatt catgttagaa aatgtaccgg tacaattcta
    29341 aaaaagtata aaattgtacc atataaaatg aaaattttgt ataataatcg tatagaaaat
    29401 aaataggagc tgatatatat gaataagaag attaattatg caccaggacc aactgaaact
    29461 agggaaaatg taagattagc aagagcagaa aaaacaacaa atccagatat agatattgat
    29521 tttgttgaat tttataaaaa gacatgcgag aagtttggta atataattgg aacaaaaaac
    29581 gatgtttaca tattgagtgg tgaaggaata ttaggtcttg aagcagcatg tgcatcactt
    29641 acagaaaaag gagatagagt acttgtaatt gacaatggta tatatggaga aggttttaaa
    29701 gattttgtaa caatgtatgg tggggagtat gtgctttttt caagtgaata tactaaaagt
    29761 atagatattg atgaattgaa agcattttta gatgaggata gtaattttaa atatgcaaca
    29821 gtagtgcatt gtgatactcc aactggcgta ttaaatgatg taagcaaaat atgtccatta
    29881 cttaaagaat atggaatatt aactgtagtt gattctgtag caggtatggt aggagaaaga
    29941 ttaagtgtag atgaatctaa aatagatata atattaggtg gttcacaaaa agctatttct
    30001 gcaccagcag gacttactat agtcggaata agtcaagatg caaaaaattg tataaaaaat
    30061 agaaaaactg atgtaatagg tttttattgt aatttaaata tatgggaagg ctattatgaa
    30121 aaaaaatact tcccttatac aatgccaata agtgacataa tggggcttga taaagcactt
    30181 gataatatat tagaagaagg aatagaaaat gtattaaata gacatgaaaa gatagcttat
    30241 agtgttagaa aatctattga ggaatatggt ctagaactat ttttagaaga aggatattct
    30301 aatacagtca cagctataaa agttcctgaa agtataggag ctttagaact tactgattat
    30361 atgcttaaaa attataatac acttgttgca acatcactaa atcaatatat ggatactatt
    30421 ttaagaatag gtcatatggg agagaatgca aatctaaata aaatagaaca tgtactaaat
    30481 gttttggata aaagtttaag tgcattagga ttcaaaggaa atggaaattt gttaaattta
    30541 tttaataagt attattttta atagataggg ggatatagta tggaagttac agttaagcaa
    30601 aaatttacaa cgaaagacat ggtggaaacc tcactattaa tagcattagt atttattgca
    30661 actaaattta taaacataag attaccaata tctataaatg gtgggcttgt gcatttagga
    30721 actgcaatgt tgttcatatc agcaatagta tttggaggta aaaaaggtgc tttatcagga
    30781 gctattggta tgtcactttt tgatttgata tctgggtgga ctttatgggc accgtttacc
    30841 tttatagtta gaggagttat gggttattta cttggaaaga ttgcatgggc aaatggtaat
    30901 aatgggaata attttttaat caatgtaata ggaatatgtg tctcatcaat ttggatgcta
    30961 tttggatatt atgtaacaga agttatactt tatggtaatt ttatagtacc tcttacttca
    31021 ataccaggta atttaatgca agtattgata ggccttataa tagcgcttcc tatttctaag
    31081 gtattgaaaa aatgtataaa ataaatataa agtgaactaa aatttatgat agcataaatt
    31141 atactgtata atattaggat ggagtacata attaaaaaat tttagtatag aggttagttc
    31201 acaatgttat cagttacaag atattcaatc aaaaatgtcc gtttaaagcc gtgggtaaag
    31261 tttatttggt attttgagac aaaatcaaat atactgttaa ataataagtt acttccgaca
    31321 gacagtatag atattattct caatctttct gatgttatgg aatataaaat tgaaaatcaa
    31381 gactatactg ctagtaatat gcattttaat ggaataaggg ataaacatgg atttattatt
    31441 caacatggaa atatacgtgt tatagggatt tctttttatc catttggtct ctatccattt
    31501 ttaaagatac caatttcaga gtttaacaga caaattgttg acctagaagc agtatcacag
    31561 ctatttgcaa aaaaattgga agagtcatta aatccaacac aatcagtaga aaaaattgtt
    31621 ttatatttag aagaagtatt attatccata ttagaagaag atttaatttc aaataaatat
    31681 gtaaagcttc taaattcatt tatatatgtt aatagatata gcaatataaa agcattttgt
    31741 gatgatacaa atataaatat taaaacattg gaaagaatct gtttaaaata tacagggtat
    31801 acacctaaga ttttaaagag aatttacaga tttaaaatgg caagcaatca acttatttat
    31861 aattataaag atgatgaact ttttgatttt atttatgaaa atgagtacta tgaccaagca
    31921 cactttataa aagaatt
//
LOCUS       DAEMHW010000028        31519 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEMHW010000028 DAEMHW010000000
VERSION     DAEMHW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.028"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            136..327
                     /locus_tag="KPK67_001064"
     CDS             136..327
                     /locus_tag="KPK67_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBG2458060.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            482..1891
                     /locus_tag="KPK67_001065"
     CDS             482..1891
                     /locus_tag="KPK67_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2458061.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNILKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNVLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            2022..2711
                     /locus_tag="KPK67_001066"
     CDS             2022..2711
                     /locus_tag="KPK67_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBG2458062.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2714..3649
                     /locus_tag="KPK67_001067"
     CDS             2714..3649
                     /locus_tag="KPK67_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBG2458063.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENSLNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3646..4539
                     /locus_tag="KPK67_001068"
     CDS             3646..4539
                     /locus_tag="KPK67_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBG2458064.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4617..5363
                     /locus_tag="KPK67_001069"
     CDS             4617..5363
                     /locus_tag="KPK67_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBG2458065.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVSCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5364..6197
                     /gene="rsmI"
                     /locus_tag="KPK67_001070"
     CDS             5364..6197
                     /gene="rsmI"
                     /locus_tag="KPK67_001070"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBG2458066.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6220..6924
                     /locus_tag="KPK67_001071"
     CDS             6220..6924
                     /locus_tag="KPK67_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG2458067.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEDGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7112..7540)
                     /locus_tag="KPK67_001072"
     CDS             complement(7112..7540)
                     /locus_tag="KPK67_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG2458068.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7577..9007)
                     /locus_tag="KPK67_001073"
     CDS             complement(7577..9007)
                     /locus_tag="KPK67_001073"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBG2458069.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9139..9723
                     /locus_tag="KPK67_001074"
     CDS             9139..9723
                     /locus_tag="KPK67_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBG2458070.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9739..10260
                     /locus_tag="KPK67_001075"
     CDS             9739..10260
                     /locus_tag="KPK67_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HBG2458071.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10326..10572
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10656..12593
                     /gene="metG"
                     /locus_tag="KPK67_001076"
     CDS             10656..12593
                     /gene="metG"
                     /locus_tag="KPK67_001076"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBG2458072.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12623..13393
                     /locus_tag="KPK67_001077"
     CDS             12623..13393
                     /locus_tag="KPK67_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBG2458073.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13730..14161
                     /gene="phnG"
                     /locus_tag="KPK67_001078"
     CDS             13730..14161
                     /gene="phnG"
                     /locus_tag="KPK67_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBG2458074.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14176..14751
                     /gene="phnH"
                     /locus_tag="KPK67_001079"
     CDS             14176..14751
                     /gene="phnH"
                     /locus_tag="KPK67_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBG2458075.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSTINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14754..15839
                     /locus_tag="KPK67_001080"
     CDS             14754..15839
                     /locus_tag="KPK67_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBG2458076.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFISELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQTLTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15859..16728
                     /locus_tag="KPK67_001081"
     CDS             15859..16728
                     /locus_tag="KPK67_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBG2458077.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16721..17587
                     /locus_tag="KPK67_001082"
     CDS             16721..17587
                     /locus_tag="KPK67_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2458078.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17628..18341
                     /locus_tag="KPK67_001083"
     CDS             17628..18341
                     /locus_tag="KPK67_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2458079.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFIMAKGTISESGVA"
     gene            18342..19511
                     /locus_tag="KPK67_001084"
     CDS             18342..19511
                     /locus_tag="KPK67_001084"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBG2458080.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPSNGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVLNTIVNGKTVYKREV"
     gene            19515..20351
                     /locus_tag="KPK67_001085"
     CDS             19515..20351
                     /locus_tag="KPK67_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG2458081.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKDDLFKFRLALGG
                     VL"
     gene            20352..21347
                     /locus_tag="KPK67_001086"
     CDS             20352..21347
                     /locus_tag="KPK67_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2458082.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     PLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLDVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21365..23053
                     /locus_tag="KPK67_001087"
     CDS             21365..23053
                     /locus_tag="KPK67_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG2458083.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTNGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVVKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23065..24132
                     /locus_tag="KPK67_001088"
     CDS             23065..24132
                     /locus_tag="KPK67_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2458084.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKDGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24569..25564
                     /locus_tag="KPK67_001089"
     CDS             24569..25564
                     /locus_tag="KPK67_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2458085.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHDGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSDIGKKVRDILEVVELSGYDKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIKSLHRKLGITTIMVTHDQEEALCLADKMVVMNNGEVIQIGTP
                     KEVYKSPKTPFVADFIGTINFIDDDVNMIAIRPEDIKVEFNRDFKEIDVKVGEILDIE
                     FRGFNYRITVEYCSKQIKLDVVSKVVEQMKLCVGSKINFKIPKERTIQYKSEGCA"
     gene            25582..27270
                     /locus_tag="KPK67_001090"
     CDS             25582..27270
                     /locus_tag="KPK67_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG2458086.1"
                     /translation="MITIKNKRRISDSKIQKCILVFLVIALVVFILFPLISLLKNAFM
                     DTSGSFVGISNFSKYIENPTLIASFKNSIFVSTISSLISLCLAFIYSYAITRTKIRFK
                     NFFKTMGIFPLFAPAMLYGISLIYLFGNKGIFTIIGIEVPLYGPLGIIICQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFFKITIPGIKYSLVSSFFVSFILAFTDF
                     GAAKIVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTVDKIASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIACSFITLLIFILIFVSVIGSVIKLWPYDLSFTMEHY
                     TFKGISGNGIKTYLSSLKISILTAIFGTIFVFFSAYMIEKVDKFPRLRQISYFLSMIP
                     MALPGLALGISYITFFNSASNPLNFLYGSTTIMVIANIVHFYSVCFITSNSSLKMLDR
                     EYELVAKSINIPFYKVFFYVTLPMSITSILEVAVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLEEIGYIGPSAAMAVLVFLTNIIVKLLYELTTKKLKTKTQKWQQKEVA"
     gene            27282..28349
                     /locus_tag="KPK67_001091"
     CDS             27282..28349
                     /locus_tag="KPK67_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2458087.1"
                     /translation="MKFRKLVSLGILSTLLLSVITGCNNASSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLTTFKEKYPDIKLNVLRLSVGDITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPVFKDDKKVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIETGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            28541..29071
                     /gene="rnmV"
                     /locus_tag="KPK67_001092"
     CDS             28541..29071
                     /gene="rnmV"
                     /locus_tag="KPK67_001092"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBG2458088.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            29242..30111
                     /gene="rsmA"
                     /locus_tag="KPK67_001093"
     CDS             29242..30111
                     /gene="rsmA"
                     /locus_tag="KPK67_001093"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBG2458089.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKNYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            30177..31442
                     /locus_tag="KPK67_001094"
     CDS             30177..31442
                     /locus_tag="KPK67_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458090.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDSDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQ
                     GESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYL
                     GYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGF
                     LRYQACDNRTNSLGYWLCFIDSRQRNIVR"
BASE COUNT        12625 a         3468 c         5665 g         9761 t
ORIGIN      
        1 taggacgtag ccacgtaatt ttttttattt tttgtcaaaa aaaacaaaaa atacgtattt
       61 cttttttcat ttcgggaaaa ctaaacacag gaattatgga ctttataaaa atttaattct
      121 tgaggagtga tataagtgaa aaaagttgct ttaattttag ctataatagg tgcgcttaac
      181 tggggtgcaa ttggtattct aggtacagac ttaataggta gtatctttgg tggaacatat
      241 gaaatggtaa gtagaataat atacttcatt gtaggtttag ctggattata cttaatacca
      301 agtttaatgt ctgatgatag agaataattt atgcttttat gttaaatacc tcattctaaa
      361 tgtttagaat gaggtattta attttataaa aatattaatt tgtttctttt ttaatggtaa
      421 aatagataag aattgaagtt ttaaaaagaa gtattatgta aaaaaggagt actattaata
      481 tatgtttatt attaatgaat taaataagat tgtaaatgaa gaagtaatat cttttcatat
      541 gcctggtcat aaaaagggac aggtttataa aaagttagga tataaagata tactaaaaaa
      601 tttgtataaa atggatacaa ctgagatatt aggaacagat aatttacact ttccggaagg
      661 aattataaaa aattcacaaa aaaaagcatc aagagtattt aaaagtgatt atacttgcta
      721 tttagtaaat ggtagtagct gtggaataca atctgcaata atgtcagtat gtagtcctaa
      781 aagtaaaata ataacaaata gagactgtca tcagtctgtt ataaatggat gtattttagg
      841 agatatagat atagagtatg tgccttgtga gataagtgaa gagacaaata tcttaaaagg
      901 gatatctata agtaatgtga ttgatataat agataaaaac ttggatgcaa aagcagtgct
      961 gcttacttat cctacatatt atggaatgac atatgattta gaatacatat gtaattatgc
     1021 acatagcaaa aagatggtag tgatagtaga tgaagcacat ggtgcgcatt tagggcttag
     1081 tgagaggctt cctaaaacag ctcttgagca aggtgcagat atagttgtac agagtacaca
     1141 taaaactctt ccagcattta cacaatcgtc aatgatacat atacagggag accgagttga
     1201 tatagataaa atattgaacg tgttaagaat tacagaatct tcaagccctt catatttatt
     1261 attatcttct ctggaattag cagtagatat atatgaaaat aaaggaaaag aattaatgga
     1321 agagctttta aataatatta taactttcaa aaataaagtt gattcaaata ttaatatata
     1381 cgacacaaat gataaaacaa aaatatttat atcatctaaa aacatgggtt taacaggtta
     1441 tgaactagaa aatattttga gaaacaaata taacatacaa gtggagttat ctaattatta
     1501 tggagttctt ttaatatgta caataggaaa tactgctgag gatttctcta gtttagaaac
     1561 agcattaaac aatgttgtaa aggaagactt taaatttgaa aagttagaaa ataaaaaata
     1621 ccctgttgtt attccaaaga aaatattaac acctagagaa gcattttata aaactaaaaa
     1681 aagtgttaaa atatatgaca gcataggtaa agtttgtggt gaatgtatag ttccataccc
     1741 acctggaata agtgtaatat ctcctggtga agtaataagt aaagaaataa tagactatct
     1801 aatgttttgt cattctaaag gcatgattgt gagtggattg aaagatgtag atttaaattt
     1861 tatacaagta atagatttag aagacattta aaaattatga tataaaagta gatagaagta
     1921 gatagtttaa aattgtgaag agaaataaat agactgctta tatagagcta tctaataaaa
     1981 gtactaaaaa agtaaaaagt gtttgtatgg aggatatgaa aatgaaaggt aagttaataa
     2041 ttatagagag tggttcagat gcaagtggaa aggctactca gacagaaata ttatatgaaa
     2101 gattaaaaaa agaaggctat atgattaaaa aggtagaata tccaaattat gaatctccat
     2161 catctgcctt ggtgaaaatg tacctaaaag gagactttgg taaaaatgca tcagatgtag
     2221 acccatatat agcatcaact ttttttacag ctgataggta tgcatcattt aaaaccgatt
     2281 gggaggagtt ttacaatgaa ggtggaataa ttatcgcaga tagatataca acctctaata
     2341 tggtacatca ggcctctaaa atggatattt gtgatagaga agagtattta aattggttag
     2401 ctgattatga atttaattta tttaagatac cacaacctga ttgtgttata tttttagatg
     2461 tgcctattga atttagtaaa aagcttatgg agaatagaaa aaataaaatt acaggggaag
     2521 aaaaaaagga tattcatgaa agtgacatag agtatttaga gaaatcttac aacaatgctt
     2581 tatatatagc agataaatat gattggaaaa aaataaattg tgttttagat aacaaattaa
     2641 gaagtattga aagtatacat aatgaaattt ataaaatagt tcttgattca ataaaaccta
     2701 tggagagata aatatgtatt ttgaaaatat tatagggcaa gattttgcaa agaaatattt
     2761 aacaaattct ataaagaaaa ataaacttaa taatgcgtat atgtttgaag gaatggatgg
     2821 aataggaaaa aagaaatttg cagatgaatt atcaaaacta ttacttgatt atgaaaatct
     2881 cgaaaatagt ccagactatg tactcataaa accagatgga aatagtataa aaatagctca
     2941 aatcagaaat ttacaatcag atatagtaat aagacctcat aaagactata aaatatatat
     3001 aatcaataat gctgaaaaaa tgactgtgga agctcaaaat gcacttttaa agacgctaga
     3061 ggaacctcca aactatgcta taataatctt agtcacaaac aacaaggaat ctttattaga
     3121 aactataaaa tctagatgtg atataataaa gttctcacct ataccaattg aagacttaaa
     3181 acgttatttg ataaatacag gtatagaaga agaaagagct caattattgg ctattttttc
     3241 gagaggaagt attgagaatt ctctaaatct atctcagtct tctgagtttt caatgatgag
     3301 agaagatata caacagtata ttcagataat gttagataaa aacatagtgg agattttaaa
     3361 tataccaaat aatatggaaa aatatagagg taagattata gctttattag atatgatgat
     3421 aaactatttt cgagatatca tcctattaaa agaaaatgta aacaagaaca tgttaataaa
     3481 tgtagataag ctagttttca ttcaaaatat gagtggaaaa attagttatt ctcaattgtc
     3541 caagattatt gatataatag aagatgcaaa gagtaaaata aaaagcaatt gcaatttcaa
     3601 tataagcata caagttatgt ctttaaatat atacgaggtg attaaatgat aaagatagta
     3661 ggtataaggt ttaaaagtgc tgggaaaata tattattttg accctgttga ttttaatata
     3721 gagcaagata tggatgtagt tgtagaaact gcaagaggct tggagtatgg gaaagtagtc
     3781 gttggtccaa aagatatgga tgaaagcgaa ttagtttctc ctttgaagcc aattataaga
     3841 atagctacag aagaagataa aaaagtatat ctagaaaata aggaaaaggc aaaagaaact
     3901 tttgagttgt gccaacaaaa aataaaagaa catgagttaa caatgttttt aatagattgt
     3961 gagtatacgt ttgataggaa taagttgatt ttttacttta cagcagaagg aagaatagat
     4021 tttagagaat tggtaaaaga cttagctgct atatttaaaa ctagaataga acttagacaa
     4081 ataggtgtca gagatgaagc taaatctata ggaggtctag ggccttgtgg tagaaaactt
     4141 tgttgttcat cttggttagg tgattttcaa ccagtttcaa taaaaatggc aaaagaccaa
     4201 agtctatctc taaatccaac aaaaatatct ggaatatgtg ggagattatt ctgttgctta
     4261 aaatatgagc atgatgtata tagtgaagca atagaaaaaa tgcctgttgt aggtgcattg
     4321 gtgcaatcag atgatggaaa aggaaaagtt gtagaagtga atcctttatt agaacaaatt
     4381 aaagtggaat ttcaagacaa gacagtaaag atatacacaa gagaagatat aaaaatactt
     4441 agagagccta aaaaatgtga tggctgtggt ggtaaatgta aggataatga tggattagat
     4501 gaagccacac taagagagct aaagaaatta gaagattaaa tttaaggtag ggcttttcct
     4561 acctttttta aagcaattaa tgcttaaagt tattaatatt tgaggagaat ttatatatgc
     4621 aattaaaaga ttcagaaaga atagatgatt tacaattaaa aggccttaaa attatacaag
     4681 atactaatgg tttttgtttt ggtatagatg cagttttatt agccaatttt actaaaataa
     4741 aaaaagatgc caaagtagta gatttgggta ctggaactgg cattatacct attttgatag
     4801 ctggaaaaag tgaagctaaa aaaattattg gcgtggaaat acaagaagat gtatatgaaa
     4861 tggcaactcg ttctataaaa ttaaatggat tagaagaaag agttgagatt ataaatgaag
     4921 atataaaata catagacaaa gtcttagaca taaatggtta ccatgtagtc acatcaaatc
     4981 ctccatatat gcatattgat ggaataaaaa atccaaatga taaaaaagca atatcaagac
     5041 atgaagtaag ttgtaatttg gaagatgtaa ttagagctgc atccagattg gttatgccta
     5101 gaggaaaatt cttcatgata catagaccaa caagattggt tgatataata actttaggga
     5161 gaaaatataa attagaacca aaggttatcc aatttgtgca tccaagacct caaaaagcac
     5221 cgaacttagt tttggttcaa tttgtaaaag atggaaggcc agagcttaaa atattggacc
     5281 cattgtatgt ttatggagaa gatgggaatt atacaaaaga attaaaagca atatataata
     5341 ataaagatat aggagagttg taaatgagcg ggaaattgta catatgtcca acaccaatag
     5401 gaaacctaga agatataaca tatagaactt taagaacatt aaaagaagta gacttgattg
     5461 cagcagaaga tacaagacat agtataaaac ttttaaatca ttttgaaatc tcaaaaccac
     5521 taactagtta tcatgaacat aataaggatt caaagggaga ttatttgata aataaactaa
     5581 ttgatggtga aaatatagct ctgataagtg atgcaggtat gcctggtata tctgacccag
     5641 gagaagagat aataaaacaa gctatacaaa ataacataga gatagaagta ttgccaggtg
     5701 caacagcctt tgtaacggct ttagttggct ctggtatgga cacacataga tttgtttttg
     5761 agggcttttt agatagagat aaaaaagtta ggaaagcaca attggaagaa ttaaaggaag
     5821 aaagtagaac tataattttc tatgaatcac cacatagatt gaaggataca ctaaaagata
     5881 tgcttaaaat tcttggtaat aggaatattt ctataaatag agaacttaca aaaaaatatc
     5941 aagaaataat aagagaaaat atagaaacta ccataaaaat atttgaggaa aaagaagtaa
     6001 gaggagaatt tgtattaata gtagagggtt ttcatggtga gaagtcaaag aaagaaaatt
     6061 atgatgggct tacagatagg gaatatgttt taaaactaat tgaaagtggt atgaacaaaa
     6121 aagatgcaat aaaaatagtt tgtaaagata gaaagttaaa gaaagatgtg gtttataaac
     6181 aagttttaga tttataaaac ctgaatctga ggtgatttta tggaaatagg gaaaattata
     6241 atagagagtt taaagaggga agatacacat aaaatattta tagaattaga taataagcat
     6301 gaattagaaa aaataattcc taaaataagc tgtatgaagt ctgtaggtga atgtaaatat
     6361 catgtagtaa attgttttga acattctata aatgcattaa aagaattaga aatagtatta
     6421 aatgataagg atttttttcc aatacatcta agaaaacatg tgtcaaacta tttaaacaca
     6481 tatattgaag acggaataaa taagttacat gtattaaagc tgggaacatt tttacatgat
     6541 ataggtaagc ctgacagtat gaccttagat gaaacaggaa gagttcattt cacaaatcat
     6601 gaaaaaatag gtgctcaaat aattgacaat atgggaataa agttagagtt atcaacagag
     6661 acatatagac ttataagtaa atatgtaaga tatcatatga tacttttgtc tctatataag
     6721 aaaaatgatt tgagtaggaa agaattaatt aatgtattta acttagtaga tgaggacact
     6781 ataggtgtaa taatgcttgg gtatgcagat atagtgtcta cagtaaaatt attgggcaag
     6841 agtggagaag tcagtgtgtt aaagacatat atggaatata ttttaacaaa ttacttgtat
     6901 aagagtaatt atacacatgt ataaatttag ttaatataat tgagttttag atgattaatt
     6961 gaagtttgaa atatacggat ttcaaaaaca taaaagaagt tgtctttgtt gtaaaattat
     7021 gaaagacaac tttttttata tcaaaaattg aaattttatt aaggatactt aatggtctat
     7081 taattaataa accattagta tttaatttat attacaatat tgattttgct tgtattggcg
     7141 ttgatagtat aacagaggtc ttagtagaac caattttctt tatagtatca atcacatttt
     7201 ctagctcgta catatctttt acaattactt ttaaaagtaa gcaatcatca cctgttatat
     7261 ggtgacattc tacaatccta gggtcctttg cagctgactc aataaattct gtatatccat
     7321 tgcttggaag agaaatatga ataaatgcct ttataactct gcctaatgaa tctgggttga
     7381 caatagcttt atatccttct ataacaccag actcttctaa tcttttgact ctttctgaaa
     7441 ctgcaggaga agttaaacca actatttttc ctaagtcctt catagaaatt ctcccatcat
     7501 cctgtaaaat ttctatgatt ctgtaatctg taacatccat aaataaacag tcccccttaa
     7561 ataaaaataa ttaatgctat aaaacttttt tagaatctat catttgtgtt tttaaatacc
     7621 aaagatttct agataaatca accttataaa tgtgtggttt tgaaagtctt ttgtattgtt
     7681 cccagatagt acttaattca tattgtacat gattttttat ttcaagaaca gcttttttat
     7741 tatatttgca tctgcctttt atatacaatg gtttaagaag ctctttaact ttgtaatttg
     7801 taaatacctt cgttttccat gtataagtag gatggaagat ttctaaaggt ttagactcat
     7861 ctataacttc atcatgtaac ataatcaaat ctgcctctgc cttattgtct tcgttatata
     7921 ttcttataac tttcttgtaa ccaggattat ttattttttc tggattctca gaaatcttta
     7981 tcttaggttc aagaactcct ttttcacaag atgcagctaa tttataaact cctccaagag
     8041 aaggtgaatc gaaagatgta atgagctttg tacctactcc ccatgaattt atagtagctc
     8101 cttctgcttt aagtgatgtt atggtatatt cgtctaggtc attagatgca gtgatgctga
     8161 tatctgaaaa gccagcgtta tctaattgtt ttttagcttc tttagataaa taagctaaat
     8221 ctccagagtc aagtcttata cccataggtt tgtatccttt ttcagaaata tttttgaata
     8281 cttttattgc attaggaact ccactgttta atacatcata ggtatctaca agtagtaggc
     8341 atttatctgg ataaacatca gcatatgctt ggaatgcttc aagttcatta tcaaattttt
     8401 gtacccaact gtgagcttgt gtaccaatta taggtatatc aaacattttc cctgctagta
     8461 cgtttgcagt tccagcacaa ccaccaacta cagcagccct agctccatat atccctgcat
     8521 ctggaccttg tgcacgacgt aatccaaatt caaatacagg gtctccttga gctgcaaagc
     8581 aaactctaga ggctttagta gcaataagag actgaaaatt aactattgtt aacaatgctg
     8641 tttcaataag ttgagcttga tataaaggtg ccttaacagt aattaatggc tcattaggga
     8701 acatgatagt tccttcctca acagcatata tatctcctgt gaatttaaat tcttttaaaa
     8761 attttaagaa cttgtcagaa aataaattta agttttttaa gtactctaaa tcttcatctg
     8821 aaaaatgaag attatctatg tattcaacaa cctgatcaat accacaaact atagtatacc
     8881 ctccatcaca agcgtttttt ctaaaaaaca tatcaaatac aacaatgtct tcatgaatat
     8941 tcttttcaaa atagccattt aacatagtaa gctgatataa atcagtaaga agtgttagat
     9001 ttctcattat ttctctcctt aattattatt aaatattaaa aattgtaaaa actcccacac
     9061 ttatactaaa acataatata gtacaacttt caagtataaa gtattaccta actatcataa
     9121 aattatttaa agaggaggat gattataata ggaaaaatat ggttttatat ggtatcgata
     9181 ggaataatag gaagcttgat ttctaaaaat atgggtgaat taaataaagt tatattaagt
     9241 gaaacatcaa aaggtataga gtttgcaata agtctagctg gtatcatggc actctggatg
     9301 gggataatga atatcgcaaa agaatcaggt ttaattgaaa agataggtca aaaactaaat
     9361 cctataatga gaagattatt tccttctatt ccaaagggtc ataaagcaat gtcttatata
     9421 gttatgaata ttgctttaaa tatgttgggt gctggaaatg gtgcaacagc atttggttta
     9481 aaagcaatga gtgagcttca aactttgaat aacaagaaag atacagccac taatgatatg
     9541 attatgttta tggtaatcaa tatatcatct atacaaataa ttccatttac tataatcaaa
     9601 ttaaggatgg atatgggggc tcaaaatcca agtgaaataa tttttacgac tttatttgca
     9661 accataatat ctacagcagt agcaataata acatgtaaat tttttcaaag aagatataga
     9721 taaaggggat gtagatacat ggagatattt tcaaacttac ttataccaat aataattctg
     9781 tacatagtaa tttatggtaa atataaaaaa atagatgtgt atgatagttt tgtaaaagga
     9841 gcaattgatg gattaaaagc tgcatgggat atcctaccat atataatagg tatatttttg
     9901 gcaataggaa tattcaaaac tggtaaaggt cttgatatgt tagaatggct atttacacca
     9961 atagcaaata tgatgagtat accaaaagaa ttaataggtc taataagtgt aaaaccttta
    10021 tctggaagtg gtgctttagg gatgtacagt gaacttgcaa atagagttgg aataggaagt
    10081 ttagtagaaa aaatgggtgc tacgattgta ggttcttctg agactatttt ttacacaatg
    10141 gcaatatact atggaagtct gaagataaaa aatactagac atacattatc atgtgctatg
    10201 attagccatg ttgcaggtgt catagcagct gtttttattt gctatgtaat atttgtataa
    10261 tttgttgaca atatgggaaa aattttataa tatagtgatt atatagaaac aaatagtaat
    10321 acaaattaca agctatgaaa agaagagtaa ataaaggttt tttctatcag agagctaggt
    10381 taggtgaaag ctagtgtcaa aagctttatt gaagatggtc ttagagcttt ttatctgagc
    10441 aagtgtgcta gggtaaaacg gttggacacg ttatagtcta ctaaggtatc tataaaatgt
    10501 ttctacattt catgtgaaac ataactatta tagatgaatt agggtggtac cgtgaataat
    10561 ctcgccccta tgttaactca taggaggcga ttttttatta tttaaatgaa agaatcatat
    10621 gattacgaac gataaaaaat taggaggtaa ataacatgag caaaccgagt ttttatgtaa
    10681 caacaccaat atactatcca agtggaggat tacacatagg acatacttat tcaacagtag
    10741 ctgcagatac aatagctaga ttcaagcgtt tctgtggata tgatgtaaag ttcttaacag
    10801 gaacagatga gcatggtgaa aaaattcaaa agaaagccat agaacaagga atgtcagaga
    10861 tagagtatct tgatggaatg ataaaagata taaaggcctt atggaatact atggatatat
    10921 cttatgatga ttttataaga actacagaaa aaagacatac agacataata caaaagatat
    10981 ttactaaact atatgagcaa ggtgatatat ataaaggtga atatgaaggt agatactgta
    11041 ctccttgtga gagtttctgg acagaatctc aattactaga aggaaataaa tgccctgact
    11101 gtggaagaga aacatattta gtaaaagaag aatcttattt ctttagatta tctaaatatg
    11161 aagacagatt aaaagagtta tttaaagatg atagtttctg ttttcctgct gctagaaaaa
    11221 atgaaatggt ggcaaacttt ttagacaaag gattagaaga tttaagcgta actagaacga
    11281 cttttgactg gggtataaaa gttccttttg atgaaaaaca tgtaatttat gtgtgggtag
    11341 atgctttatg taactatata acagcattag gttatatgac tgataatgat gaagaattta
    11401 aaaaatattg gcctgccaat gttcaaatag taggtaaaga aatagtgaga ttccatacaa
    11461 taatatggcc agcgctttta atggctttag gtctagaagt tccaaaacaa gtatttggac
    11521 atggatggat actatttgct gatgataaaa tgagtaaatc aaagggtaat gtggtttatc
    11581 cagaacctat aatagaaaga tatggaatag acacacttaa gtacttctta ttaagagagt
    11641 ttgcatttgg gcaagatgga agttatactc atagaaattt tgtaacgaga ataaattatg
    11701 atttagcaaa tgatttagga aacttaataa gtagaactgt agctatggtt gaaaaataca
    11761 ataatggtat aataccaaca gcaaaagttt caactaattt tgatgctgac ttaaaagaac
    11821 aagctgtttc aactagagaa aactttgaag ctgaaatgga taaaatgcaa ttccatgaag
    11881 cattagagag tgtatggaaa ttagttagaa gaactaacaa atatatagat gaaactatgc
    11941 cttgggcact agctaaagat gaaacgaaga aagatgaatt agacacagtt ttatataatt
    12001 tatgtgaatc tataagaata atcgcaacac taataaatcc tataatgaat gagactgcta
    12061 ataaaatata tgaacatata ggaataaaag gtcaagatga tataactaca tgggaaagta
    12121 caaaaacatt tggtcttatt ggagaaaatg taaaagtatt taaaggtgaa cctttattcc
    12181 caagattaga tgtagaaaaa gagatagaag aattaactaa aatgttctct gggaaacctc
    12241 cagtagaaga aaaaccttta gagcataaag aagaaattac tattgatgat ttagacaaaa
    12301 tagagcttag agtagggaaa ataataagct gtgaaaaaca tccaaaagca aataaattat
    12361 tagtatcaca agttaagatt ggaccagaaa caagacaaat agtatcagga atagctgaat
    12421 actataagcc agaggattta gtaggaaaag aggttacagt ggtatgtaac ttaaaaccag
    12481 ttaaattaag aggtgtagaa tctcaaggta tgatattagc agcaggtgat gatggagacc
    12541 catatgtttt accatttaca caaggggcta aagatggatg cgaggttcgt tagtaggtaa
    12601 ttaaatagta aggagattaa agatgttatt tgactcacat gcacatttaa atgatgaaag
    12661 ttttgatgaa gatagagatg aacttattgg ttcattaaaa gataaaggtg tagatttggt
    12721 tgtaaatcca ggagcagata tagagacgtc gataacagca attgaacttg cgaaaaaata
    12781 cgactttata tattcagcag ttggggtaca tccacatgat gtatcaaaac tagatgatac
    12841 agcaatagaa actctaagaa aacttgcaac tgagaatgaa aaagttgtag ctattggaga
    12901 gattggatta gattattatt atgattattc tccaagagaa gaacaaaaag agtggtttaa
    12961 gaagcaaatt gaattagcta atgagctaaa acttccaata ataatacatg atagagatgc
    13021 acatggagat acttttgaga taataaagaa tactaaaaat cctgaaatag gatgtgtact
    13081 tcattgttat agtgggaatg tagagttagc tagagaatat gttaagatgg gttgttatat
    13141 atcaattcca ggcacggtta cttttaagaa taataaaaaa actagagaag tagtaagaga
    13201 aatacctcta gaaagattat tcatagagac tgattcacca tatatgtcac cagagccaca
    13261 tagaggaaag agaaacaatc cttcccaagt atcttttgtt gctgataaga tagcccagga
    13321 aaaagggata tcttatgaag aagtatgtag agttacaaaa gaaaatgcaa agaaattttt
    13381 taatataaaa tagacaaatt aaatcttata atgatatata taataagtca ttataagtat
    13441 aggatgagag tgcctcaaaa tgagtttttt agtttatgag gcactctttt tttatgaaat
    13501 caaatatatt ttcatcattt caacaatgaa aaagaagttt atcatatttt gagatagttt
    13561 gtttttattt atttaattca tattttaaaa tatattttct taacattgtt aaaaaagaat
    13621 ttacaagata ttcattagaa ttaactcaac tctaatattc acttaactta agttgtttat
    13681 tatattatta aagagaagta aacgaaaggt agattaagga ggttgaattt tgtatacagt
    13741 tgatagtact aagatattga taaatatgaa cttagagaaa ctagaaaaga tatactcaat
    13801 gataaaagat acatcaagta tttctataat taaagaacct aacttggcta cagtaatggt
    13861 aagagctaat gaaagtgtaa aaaacacaac atttaattta ggagagatat tagttactga
    13921 atgtagtgta aaggtagatg aaagcttggg ttacggaata gtttcagaaa acaataataa
    13981 gaaagccatt tacttagcag ttatagatgc agttttacat agcaataaca gtaaatttga
    14041 tgagttaaag aattacataa ataaaagtgt atatgaagag aacttaaggt atgaacaaga
    14101 gattattgat gaatttagct tgattaataa aacgaaagtt caattcaatg ctatggatta
    14161 aataggagga gaatcatggt aaattataca caagaaatat atagaaagat gctagatagt
    14221 gcttcatttc ctgggaaatt aaataatata gataatataa atatagaaaa taatacaaaa
    14281 ctattgaatg gagcaattgg tatagcaatc actttattag accaagaagt taccttctat
    14341 atagaaaatt atactcaaaa agatataaaa aatataagag cgcttacaat gtcaaatcaa
    14401 atagattata gaaaatcaga ttatatattt ttagatatta attcagaagt ggatatatta
    14461 cagattaaaa taggttcttt tgaatatcct gatgaaagtg caactatagt acatcaagta
    14521 aatgatttat caacgtactc tcaaactaag tatataaagc ttgatttgag tggtcctggt
    14581 ataaaatcta aaaatagtat atatataaat ggaattagag aagaatttat agaaagatta
    14641 tctaccataa ataaagatta tccaatagga atagatttga ttttagtaga taaaaaagga
    14701 gaaattgcat ttataccaag gtcatcaaaa ttaagttggg aggtattgta gaaatggctt
    14761 atgtagcagt taaaggtgga gaagaagcca tattacaggc taaaaatata cttgagtttt
    14821 atagggtaaa aggagatagc ttgcctatag atgtaaagca gattgaggaa caaatgtctt
    14881 atgctgtaga caaagtaatg tctgaaggaa gtttatacaa caaggaacta tcagcattgg
    14941 ctataaaaca agctcaaggt gatatattag aagcctcttt ctatgtaaga gctttaaggt
    15001 caacaatgcc tagaattaca tattcagaac ccataaatac agaagaaatg agaataaaaa
    15061 gaagaatatc agctactttt aaagatatac caggaggtca gtatcttggg gcaactagag
    15121 actatgaaaa tagaatatta aatgtagaat tattagaaga agatgaaaaa aagaatagag
    15181 attttagaga tagttttata agtgaattag aaacagtaga tgaaatccct gaatttacaa
    15241 aaatatctaa tatatttaga aaagaaaatt tattacaaga agaagtttgt gaaaacaaat
    15301 tggaagaagt tattgatgat attacaagaa atagtttaag atttccagtt tctaggtcag
    15361 caaagttaca gacattaact agaggtgaaa gtggagtagt gtcaggtttt gcttatgcta
    15421 ttgtaagagg ttttggtgat gaacatccat atataagtga acttagaact ggtaatgtat
    15481 ttataaaagt aaaacatccc atctatgaag atgaagaaat aataataggt gatatgctgg
    15541 ttactgaatg tgaaatagtt tcaaaagcat cacaaaataa gactgaaaat tctaaaacta
    15601 attcggaaga taaaaataag atgtctttag gatatggtct ttgtatgggg aataatgaga
    15661 ataaggctat atctatggcc atactagaca aatctttgga gccaaatagt gaaaaactag
    15721 cacaagatga agaatttgta ttgcttcata tagatggaat agattcatta ggatttatat
    15781 ctcactttaa gcttcctcac tatgtagatt ttaaagcgga tgtagaaaga ataaaatagt
    15841 ttgaggaggt aagtttaggt gatatataac tatggttttt tagacgaaaa cacaaaaaaa
    15901 gaaataagaa gaaagatatt aaaagcaata tcaatacctg gttatcaagt tccctttggt
    15961 tcaagagagt tgcctatagc taagggatgg ggtactggag gtttacaatt aacactatca
    16021 ttaattggta aaaatgatgt agtaaaagta attgaccaag gaagtgatga ttctacaaat
    16081 gcttgtaata taaggagctt tattgcaagt gtaagtaatg tagaaaccac aaaagataca
    16141 ttagaagcta cattgattca aacgagacat agaataccag aagaaaaact taaatctaat
    16201 caaatactaa tatttcaagt accaatacca gaaaccctta gaatagtaga acctagtgaa
    16261 gtagaaacaa gaagaatgca ttcagaagaa gattatagta ggatgtgggt atatctttat
    16321 gaagatatag ttagatttaa tgatatatct atagcagttg aatatccatg taaagtaaat
    16381 gatagatatt taatgaatcc atcacctata cctagatttg atataaaaaa attaaatatg
    16441 tcagacaatt tattcttgtt tggtgcaggt agagaaaaaa gaatttatgc tattcctcca
    16501 tatacaaagg tagaaccttt agagtttgag gattataagt ttgaagagga aaagtttgaa
    16561 ggaaaatact gtagtttatg taacagtaca aatacatttt tagatgaagt atatgacagt
    16621 gacactaatg aaaagtacta tagttgctca gatacaagtt attgtgaaaa agttagatta
    16681 aaaaataaca gtatagatgt gacaatagga ggtacttgga atgagtaaaa taaatgactc
    16741 taaaagatta gaaaatatag acaagcaagt attaatagga agaaatatca atcttattta
    16801 tggagatggg tgtgaaaagt gttttgaaag cacaggatat gaaagcaata atatatgtaa
    16861 gtattgtggt tctatagttg catgtaataa tgtaaattta gaactttatg aaggagaagt
    16921 ccttggaatt gtaggtgaat ctggttcagg aaaaagcact ttattaaaaa tactgttttt
    16981 ccaagaacat gcaaatagtg gagaagtata tatatcccat tacaataaag acacaaatat
    17041 actagagctt tcagaccaaa aaaagagata cataaagaat cattttatgg gtattgtgta
    17101 tcaaaatccc caccttggtt taaatttaga ctttagtagt ggtggtaata tagcagaaaa
    17161 attattgatg gcaaatttat ataatgttga aaaaataaga gatagaggca aagaactttt
    17221 aataaaaact aatatacctg tagaaagaat agaccataaa ccaaaatact ttagtggggg
    17281 aatgcaacaa cgagttcaaa tagcaaaagc tctttcaaat aaccctccaa tattgctatt
    17341 agatgaggtt acaacaggtt tagatgtttc tgtgcaagcg gaagtattgg atttaataag
    17401 agaaatccaa agagaactta agatttctat gatagttgta tcccatgact ttgatgttat
    17461 aaagatgctt gctgatagga caattgtaat gaaaaatgga agtgtaattg agtctggtct
    17521 tacagaccaa ataatggagg acccacaaca cccatatact cagcaactta taaattcatt
    17581 gctataaata tcgtttgtaa ataagcaaaa tgggggtttt taataaaatg aaagaaacta
    17641 tacttgaagt aaataatttt agtaaagact ttgagttaca tgcattgaac aagactataa
    17701 cggcatgtag taatataagt tttgaagttt caaaaggtga gtttttagga ataataggta
    17761 aatctggagc aggtaagtct accatattaa aaagtatcta taaaacatat ataccaacaa
    17821 ctggagaaat aatttttaat tcagaaatat atggtaatgt agacctggct aaaattggag
    17881 accgagaaat gataaatctt agaaagaaag aaataggata tgtatcacag tttttaaaga
    17941 cacttccacg aataactgca attgaactgg tagtacattc tttaattgag agtgggtttg
    18001 aaaaagattg ttcgtatgat atggcaaaag atatacttaa ccaatttgaa ataaaggaaa
    18061 atttatggga tgcgtatcca aataattttt ctggtggaga aaaattaaga ttaaatttag
    18121 ctcaagctat ggttaaaaag cctagacttt tgttacttga tgaaccaact gcatcacttg
    18181 ataatcaatc taaaatatat gtaaaagaaa aaatgttgga acttaaaagt caaggaacta
    18241 caatgatagg aatttttcat gatatagagt ttatggaaac tgttatagat aagaccttta
    18301 ttatggcaaa agggactata agtgaaagtg gggtagccta gatgataaaa ctaattcata
    18361 atgcaaatat tgtattagaa gatatgattt tagaaaatgg tcatttaatc atagagggag
    18421 atacaataaa aaaagtatca aatgataaga ttgattacgc atttgacttt aatgagatag
    18481 atgaaataat agatgcaaaa aacttatatg taatacctgg tattatagat attcacagtg
    18541 atgccattga aaaggaaata gagccgagac caagtacatt attaccattt aatatggcat
    18601 tttatgaact agataaaaaa ctaccttcaa atggaataac tacagtttat cattctatat
    18661 ctttagggga tggagtagga gttagaagta ttgataactc tttaaagatg attaaaaata
    18721 ttgattcata taaaaatata gatagtaaga gcataaatca taaagttcat ttaagatatg
    18781 aagtgttgta ttatgaaggt ttagaaaagg ttttagaatt attagatgaa aataaaatag
    18841 actacttatc aataatggac cattccccag gacaaggtca gtatacaaat ccaacatttt
    18901 ataaagagta tgctactaaa gtgtggggag ttacagaaaa ttatgtagat acatggttag
    18961 atgatttagt aaatctgcat gataatttag attggaataa aatagctaat attataggta
    19021 tagccaaaac taaaaatata aatgttgcat cacatgatga tgatacatta gaaaaaatgg
    19081 atttcataaa aaatataggc ataaatgtat ctgaatttcc tataactctt gaagttgcaa
    19141 aacattcaaa gaaattgggt gttctaactt gtttaggtgc accgaatatt gtaagaggta
    19201 aatctcataa taacaattta aaagcaatgg atgctatatt ggaagattgt tgtgacattg
    19261 tgtgttcgga ttatcttcca tcagctatga taaagtctat gtgtatagtt gcagaaagaa
    19321 taaataattt aaataaagca gtcagtttat ttacctcaaa tcctgctaaa gcagttggta
    19381 tatatgatga aagaggtagt ataaaagaaa ataaaaaagc agatttagtt ttagtagata
    19441 ttgatagtga atatccaaag gttttaaata ctattgtaaa tggaaaaaca gtttataaaa
    19501 gggaggtata aagattgcta acagagttac attgtcatac aagtatatca gataataatt
    19561 ttactactga agaaataata aaaaaagcag cagaaaagaa tataaatatc ttagcaataa
    19621 caaatcatga cacattatct ggattggatg aagctcttgt gcttggagag cattatggaa
    19681 taacaataat accaggtata gaaatttcag cctatgatta taaaaataga aaaagagttc
    19741 atattcttgg atataacata gatttaaaca gtcaagaaat aaaaaatttg tgcaatccaa
    19801 tggtatgtga cagacataaa gcatctgtta agatggtaaa taaaattata gatttaggat
    19861 acaaaatatc tctggaagat gtaaagaaat attcatcaaa gactggaata tttaaacaac
    19921 atataatgaa agctcttata gataaagggt atacagataa tatatattca agtctatata
    19981 aagaattgtt tcatagagga aacggcaagg tatatgtatc tttaaaatat gtggattaca
    20041 gagatgcaat aaaagctata aaaaattctg gtggaatttg tgtgttagct cacccaggtc
    20101 aaatggataa ttttagcgct attgaagaga tggtaaaagt aggattagat ggaatagaag
    20161 tatatcatcc atctcacaac aaagaactag aaaaagagtc gctttcttat gctaagaagt
    20221 ataatctagt ggtcacagga ggttcagatt accacggatt ttattctcaa cataattatg
    20281 aacttggaag caagagctta aataaagacg atttatttaa gtttaggtta gctttaggag
    20341 gggtattata aatgtctttt ttacaagtaa aaaatgtagg taaaagttat ggacaagtta
    20401 aggtgttaaa agacatatct attgatattg aaaaaggaga gtttatttgt ttactaggtc
    20461 caagtggttg tggaaagagt acaccactta gaataattgc agggcttgag gataaacatg
    20521 gtggaaaaat aataataaat gataaggata tgactaattc accacctgaa agtagaaact
    20581 ttggtattgt ttttcaatca tatgcattat ttccaaatat gaatgtttac aaaaacatag
    20641 cctttggact agaaaataaa aacataagta aatctaatat tgataaaaaa gttaaagaag
    20701 ttctagacgt tgtagaatta agtggatatg agaaaaaata tccatcacaa ttatcaggtg
    20761 gacaacaaca aagggttgca ttagctcgtg cgattgcttt agagccagac tttttacttc
    20821 ttgatgaacc tttatctgca ttagatgcaa aagtaagact taaacttagg gagcagatta
    20881 gaagtttaca tagaaaacta ggcattacaa ctataatggt aacgcatgac caagaagaag
    20941 ctctttgttt agctgataag atggttgtta tgaatagagg agaaataata caagttggaa
    21001 cgccaaagga ggtatataaa aatccagaaa caccttttgt agctgatttt ataggaacaa
    21061 taaattttat agatgatggt attaataaga tagctattag accagaagat ataaaagtag
    21121 agtctaatag agattctaaa gataaggata taaaagtggg agaaatttta gatatcgaat
    21181 ttagaggatt taactataga attacagttg agtatcgttc taagcaaatg aaattagatg
    21241 tagtttccaa agttgcagaa caaatgaaac tatgtatagg ttctaaaata aactttaaga
    21301 tacctaaaga aggaattgta caatataaat cagagggatg tgcttaatag ctaggggtga
    21361 ataaatgata acatcaaaaa ataaaagaag aataagtgat agcacaatac aaaaatattt
    21421 cttgattttt tctgtaattg tactaatagt atttatatta tttccattag tatcattatt
    21481 aaaaaatgca tttatggata caaatggaaa ttttatagga atttcaaact ttagtaaata
    21541 tgttgaaaat ccaagtctta tagcatcatt taaaaattca atttttgtat ctacagtctc
    21601 atctttaata tcactatgtt tagcatttat atattcatac gcaataacaa gaacaaacat
    21661 aaagtttaaa gattttttta aaactatggg aatgtttcca ctttttgcac caactatgtt
    21721 atatggtatt tcacttatat acctttttgg aaataaggga atctttacaa ccatgggtct
    21781 ggaagtacca ctatatggtc cattaggaat aattatttct cagattctat ttacatttcc
    21841 acaagctttt ttaatattat ctgtagcact ttcgatggct gattatagac tttatgaagc
    21901 agcagatagc ttgggtgcta gtgaatttaa gaaattttgc aaaataacaa ttcctggtat
    21961 aaagtactca ttacttagtt cattctttgt atcctttata cttgcattta cagattttgg
    22021 agcagctaag gtagtcggag gaaactacaa tgtactggct actgacatat ataaacaagt
    22081 agttggacaa tttaacatac caatgggagc tactgtttct atggtaatgt taattcctgt
    22141 actaatagca tttacattag ataagatggc aagtaagaaa caaggtatga ccataactgc
    22201 aaagtctatg ccatataaga ttaaggaaaa taagtttaga gatagagtat ttttaatagt
    22261 atgtagtttt ataacattgc taatttttat tttaatattc gtttcagtta taggttcagt
    22321 tgtaaagtta tggccatata atttaagctt tacaatggaa cactatacat ttaaaggtat
    22381 tttgggaagt ggaataaaaa catacttaag cagtttgaaa atatcaactt taacagcaat
    22441 atttggaact atatttgtat tttttagtgc ttatatgata gaaaaggtgg ataagtttcc
    22501 taaattaaga caaacagctt accttttatc aatgatacca atggccttac ctggcttagt
    22561 tttaggtata tcatatataa ctttttttaa ctcagcaagt aatccattaa attttttata
    22621 tggaagtaca gcaatattag ttatagtaaa tgtagttcat ttttattcag tttgttttat
    22681 aacctcaaat tcatctttaa aaatgttgga taaagagtat gaattagttg caaaatccat
    22741 aaatattcca ttctataaag tattttttaa tataacagtt ccaatgtcta taacatcaat
    22801 acttgaaata gtagtatatt attttgtaaa ttcaatggtt actgtttcag cattaatatt
    22861 tttatataca cctcaaactc agactgcatc aatatcaata ctaaagttag atgagatagg
    22921 atatatagga ccatcagctg ctatggcagt attagtgctt ttaacaaata taatagtaag
    22981 acttttgtac gaatttgtaa caaagaaatt aaaaaataac acacaaaaat ggcagcaaaa
    23041 agatgttgca taggggaggt aaaaatgaaa tttaagaagc tagtttcttt aggagtatta
    23101 tcaacacttt tattaagtgt aataacagga tgtagtactg attcatcagg ttcagattca
    23161 aagtctgata gtaagacaaa aggggattta acagtttaca cagcaattga agaagatagt
    23221 atagagccat atttggctac atttaaagag aaatatccag atattaaatt aaatattgtt
    23281 agggcatcaa caggagatat aacagcaaga ttattagcag aaaaggataa tcctcaagca
    23341 gatgttgtat ggggagttgc agccacaagc ttattggttg cagatgacca ggggatgtta
    23401 gaaccatatg ctccaaaagg ttctgaagaa atagagtctg catttaaaga tgataaagaa
    23461 gttccttctt gggttgggat agatgcttgg atgacaggta taacagtaaa cactaaagaa
    23521 ttaagtgata agaacatccc agttcctagt tcttacgaag atttaataaa gccagaatat
    23581 aaaggtctta tatctatgcc taatccatct tcttctggta cagggtactt aacagtatca
    23641 gcccttatac aaattatggg ggaagaaaaa gcatggcagt atatggataa gttacatgag
    23701 aatattggtg tatacactca atctggttca gctccagcta catcagcagc ttcaggagaa
    23761 tatcctattg gtatatcttt tggatataga ggtataaagt taaaagagga aggctatcca
    23821 gtagaagtag tatttccaaa agacggttct ggatgggata tagaagcaaa tgctcttgtg
    23881 aaaaaagata atataaaaga agcatcaaaa gtatttttag attgggctat aagtaaagat
    23941 gcaatgaacg aatactctaa gaactatgca gttactacta tttctacagg aaatccaata
    24001 ccagaaggat ttcctaaaaa gccattagag caaatgatag ataatgattt aaaatcagca
    24061 gcaaaaaata gagaagatat actaaataaa tggatatcaa aatatgatgg taagacagaa
    24121 aaagagagtt aagtaaaact atacaaatgg agtaaaaaga aagaattttt ttaagtagat
    24181 tttattataa taatataagt tgagaataat ataggtcaag aaaagtgaat attctataaa
    24241 aatgtataga gtaagtatac cattcagttt gaatggtata ttttttattt ttagagatta
    24301 atttagcaat agatatacta cagtttaaaa tagtatattt taatttcatt attaaagtat
    24361 agatattata tataaagtaa ataaattatt gagcgattat taataagata tgtataaatt
    24421 ttaatttggg aacttgtgtt atatatcatg aaaaaagaga aaaaataaaa ggtaaattgt
    24481 ttcttgataa cttgaaattg gaagtgagaa tatttaacta gagaagaatt atttaagttt
    24541 aagtcagctt taggaggagg aattataaat gtctttttta caagtaaaaa atgtaggtaa
    24601 aagttatgga caagttaagg tgctaaaaga catatctatt gatattgaaa aaggggagtt
    24661 tatttgctta ttaggtccaa gtggttgtgg taaaagtaca ttgcttagaa taattgcagg
    24721 acttgaggat aaacatgatg gaaaaataat aataaatgat aaggatatga ctaactcacc
    24781 acctgaaagt agaaactttg gtattgtttt ccaatcatat gcactatttc caaatatgaa
    24841 tgtatataaa aatatagcct ttggattaga aaataaaaat ataagtaaat ctgatatagg
    24901 taagaaggtt agagatattt tagaagttgt agaattaagt ggatatgata aaaaatatcc
    24961 atcacaatta tcaggtggac aacaacaaag ggttgcacta gctcgtgcga ttgctttaga
    25021 gccagacttt ttgcttcttg atgaaccttt atctgcatta gatgcaaaag taagacttaa
    25081 acttagggaa cagattaaaa gtttacatag aaaactaggc atcacaacta taatggtaac
    25141 acatgaccaa gaggaagctc tttgtttagc tgataaaatg gttgttatga ataatggaga
    25201 ggtaatacag attggaactc ctaaagaagt ctataaaagt ccaaaaacac cttttgtagc
    25261 tgattttata ggaacaataa attttataga tgatgatgtg aatatgatag caattagacc
    25321 agaagatata aaggtagaat ttaatagaga ctttaaagaa attgatgtaa aagtaggaga
    25381 gattttggat atagaattta gaggattcaa ctatagaatt acagttgaat attgttctaa
    25441 gcaaataaag ttggacgtag tttcaaaggt agtggaacaa atgaaactat gtgtaggttc
    25501 taaaataaac ttcaagatac ctaaagaaag aaccatacaa tataaatcag agggatgtgc
    25561 ttaatagcta gaggtggata aatgataaca ataaaaaata aaagaagaat aagtgatagt
    25621 aagatacaaa aatgtatttt agttttttta gtgattgcat tagtagtatt tatattattt
    25681 ccattaatat cactattaaa aaatgcattt atggatacaa gtggaagttt tgtaggaatt
    25741 tcaaacttta gtaaatatat tgaaaatccc actcttatag catcatttaa aaactcaatt
    25801 tttgtatcta caatttcatc cttaatatca ctatgtttag cgtttatata ttcatatgca
    25861 ataacaagaa cgaagataag gtttaaaaac ttttttaaaa ctatgggcat atttccgctt
    25921 ttcgcaccag ctatgctata tggtatttca cttatatacc tttttggaaa caagggaatc
    25981 tttacaatta taggcataga agtaccacta tatggtccat taggaataat tatttgtcaa
    26041 atactgttta catttccaca ggccttttta atattatctg tagcactttc gatggctgat
    26101 tatagacttt atgaagcagc agatagctta ggggctagtg aatttaagaa gttcttcaaa
    26161 ataacaattc ctggtataaa gtattcatta gttagttcgt tctttgtatc ttttatactt
    26221 gcatttacag attttggagc agcaaagata gttggaggaa actacaatgt attggctact
    26281 gacatatata aacaagtggt aggtcaattt aacataccaa tgggagctac tgtctctatg
    26341 gtaatgttaa ttcctgtact aatagcattt acagtagata agatagcaag taaaaagcaa
    26401 gggatgacta taactgcaaa gtctatgcca tataagatta aggaaaataa gtttagagat
    26461 agagtatttt taatagcatg tagttttata actttattaa tctttatttt gatatttgtt
    26521 tcagttatag gctcagttat aaaattatgg ccatatgatt taagctttac aatggaacac
    26581 tatacgttta aaggtatatc aggaaatgga atcaaaacat atttaagcag tttaaaaata
    26641 tcaattttaa cagcaatatt tggaactata tttgtatttt ttagtgctta tatgatagaa
    26701 aaagtggata agtttcctag actgagacaa atatcttatt ttttatcaat gataccaatg
    26761 gcattacctg gtttggcttt aggtatatcg tatataactt ttttcaattc agcaagtaat
    26821 ccattaaatt ttttatatgg aagtacaaca ataatggtta tagccaatat agttcatttt
    26881 tattcagttt gctttataac ttcaaattca tctttaaaaa tgttggatag agagtatgaa
    26941 ttagttgcaa aatctataaa tattccattt tataaggtgt ttttttatgt aacacttcca
    27001 atgtccataa catcaatact tgaagtggct gtatattact tcgtaaattc aatggttact
    27061 gtttcagcat taatattttt atatatacct caaactcaaa ctgcatcaat atcgatacta
    27121 aagttagaag agatagggta tatagggcca tcagctgcta tggcagtatt agtgttttta
    27181 acaaatataa tagtaaaact tttgtatgaa ttgacaacaa agaaattaaa aactaagaca
    27241 caaaaatggc aacaaaaaga agttgcatag aggaggtaaa aatgaaattt aggaaattag
    27301 tctctttagg aatattatca acacttttat tgagtgtaat aactggatgt aataatgctt
    27361 catcaggttc aaatttaaag tctaataata agacaaaagg agagttaaca gtttatacag
    27421 caattgaaga agatagtata gatacatatt taactacatt taaagagaaa tatccagata
    27481 ttaaattgaa tgtacttaga ttatcagtag gagatataac agcaagatta ttagcagaaa
    27541 aagataatcc taaagcagat gtgttatggg gggttgcagc tacaagtttg ataattttag
    27601 atgacaaaga aatgctagag ccatacgctc cgaaaggatt tgataaggta aatcctgtat
    27661 ttaaagatga taagaaagtt ccttcttggg ttggaataga tgcttggatg actggtataa
    27721 cagtaaatac taaagaatta agtgataaga acataccagt tcctagttct tatgaagatt
    27781 taataaagcc agaatataaa ggtcttatat ctatgcctaa tccagcttct tctggaacag
    27841 gatatttaac agtatcagaa cttatacaaa ttatgggtga agaaaaagca tggcagtaca
    27901 tggataaatt acataaaaat attggtgtat acactcagtc tggttcagcg ccagctatat
    27961 tagcagcttc aggagagtat cccattggta tatcttttgg aaatagaggt ataaagttaa
    28021 aaggtgaagg ttatccagta gaagtagtat ttccaaaaga aggttctgga tgggagatag
    28081 aatcaaatgc acttgtgaaa aaagataata taaaagaagc atcaaaagta tttttagatt
    28141 gggctataag taaagatgca atgaatgagt attctaagaa ttttgcagtt actacaattg
    28201 aaacgggaaa tccggtacca gaaggatttc caaagaatcc attagaacag atgatagata
    28261 atgatttaaa atcagcagca agaaatagag aggctatact aaataaatgg atagaaaaat
    28321 atgatggaaa gacagaaaaa gagagctaag ccttacaata aaatttagta caaaaagcgc
    28381 ctagaatata agttttatat ctaggctctt ttgatatatt tatttaaaat gcatatttct
    28441 atataaaaat aaatagttta gtaccttgat taaattaaat aaaaacctta aaataaagag
    28501 atgtaaagaa aaactaaaaa cgaataaaag gtgaatttat atgataaaag aaataatagt
    28561 agtagagggt agagatgacg ttacagcagt taagagagca atagatgcag aacttataac
    28621 tacaggtgga tttggatttc caaaaggtgt aatggagcgt ataaaagctg ctaatgaaag
    28681 acgtggtgtt ataatattta cagacccaga ttttgcaggt gaaaaaataa gaaaaaaaat
    28741 agcatcagaa gttccaggat gtaaacatgc atttctaccg agagaggaag ctaaaaagga
    28801 tggagatata ggtattgaga atgctactcc tcaaagtata atagcagctc tgaataaagt
    28861 tagaactgaa aatacagaaa aaagagatga atttaagcaa gttgacttaa ttagaaatgg
    28921 tcttataggt aatgaagaag catcacttag aagagatgca cttggaaaaa tacttggaat
    28981 tggatatgga aatgcaaaac aatttttaaa tagattgaat aattatggag ttgaaagaga
    29041 agaatttata gatgcattaa aacaaatcta aacgtaatat ttaatgtatg aaaaatatgt
    29101 atataaaata aattgtggaa ttacggttaa tttaagataa attatttata atatatacct
    29161 tattttaaat caatgtaaat acatataaat aagtataatt aataaaatta aatatcaaaa
    29221 taagttaaaa aggagtaaat aatggataga ctatcttcac ataatgcaac aaaggaagta
    29281 gtacaaaaat ataattttaa attttcaaag tctttaggtc aaaatttctt gatagatagt
    29341 aatataattg ataaaatctt atctggagct agaatcacaa gaggagataa tatcatagaa
    29401 gtaggacctg gtataggaac tcttactcgt gagatgggta aaatagcaga aaaagtagtg
    29461 gctatagaaa tcgatagaaa tttaattcct atattgaaag ataccttgtc agatttagat
    29521 aatacagagg ttgtaaatca agatatacta aaagtagaca tacaggaatt agtaaaagat
    29581 aaattaaatg gtgggccagt taagcttgta gcaaatcttc catattatat aactactcca
    29641 atagttatga aatttttaga ggaagacata cctgtaacag acatagtagt catggttcaa
    29701 aaagaagttg cagatagaat gaatgctatt ccgggtacta aaaattatgg ggcattatct
    29761 atagctgttc aatactattg tgatacagag attgtggcaa aagctccaag acatatgttt
    29821 ataccacaac caaatgtaga ttccacagtt ataggtttac atgtaagaga taaaagaaaa
    29881 tatgatgttc ataatgaaga catatttttt aaaacagtaa aagcatcctt tggacaaaga
    29941 agaaaaactc ttcttaattc gttgggaggt cttggttttc ttaataaaga tgaaataaga
    30001 gaagtattaa aagaagctaa tatagacgaa aaaagaaggg gtgaaactct tagtatagaa
    30061 gagttttcag ttctttcaaa tataataaat acaaaagtat cttctaaata gaaggtactt
    30121 ttttttattt tgttaacata tattgtttag agaaactgtg ttaagggggg attgcagtgg
    30181 gttctaaaag aaagtttaaa agagactata taatactaga agctaaagac ataaacttta
    30241 gatacaaaga acgagtactg ccaaaagctt ttgcaaaagt tgaagtaaat gatgaaaaag
    30301 caattattgc attatatgta gaaaatctaa aatatgttaa agatgggtat aaagcagtag
    30361 caataaaaga tgaccatgga ttaatagact taggaaatat aattttaaat gaacaaggaa
    30421 gaggtgaatt tgttttagat ttagaagatt ctgatgatga gataaaaggt attgcattat
    30481 tgtatgaaag aaatatacct ttaattggat ttaagggaaa taagcttgaa aattatgagg
    30541 acatattatt tcaagaggta gaggaagaag aatatgaaga atatgaagag ttggaagaag
    30601 aagacgaaga gatagaggag aatgaggaaa gtgatagaga cgaagatagt gaagaagaat
    30661 atattataga agaatatgat gaagatgagt atgaagacta ttgggaagat gatgaagata
    30721 ttgaagaagg agagttaaaa gcaagaacgg aagtagaaga ttctggtgat gaggaaatag
    30781 aagagttcaa taattataag gaaatagaaa attataatag ggttaaagta caggaagaat
    30841 atatagacat agaatatgat gaggaagaag aggaagaaga agaagttttt gaagtaaata
    30901 gagaagaaga taggtttgtt tcctcaaaac ctactaaatc aaagaaagtt caaaaagatc
    30961 agaataatat tgaaaaaata aaaagagcta atggagtaag tcaaggagag agtggaaata
    31021 aacaaaccag tacattactt atgccaagac aaataaaaaa aggtttgaaa ttatttaaag
    31081 aagtaaaacc ttttgtaaca gattatatag ataagacaag atggtggaaa atagagataa
    31141 atcctacaac actgtgtgga tatacaatgc cttatttagg atatttaaac gtattgaatt
    31201 acactatgta tagtgatgca gttttacatt catataaata cagacactat ttatttggtg
    31261 ttcagtatga tgaatataat aagagaaaac attatattta tgctatccca ggaaatagaa
    31321 atgaacagcc agacaatgga agcacaggat ttttaagata tcaagcttgt gataatagaa
    31381 caaatagttt aggatattgg ctatgtttta tagattctag acaaagaaat atagttaggt
    31441 aaaaataata aaaatgaaaa aatttagact caattcttat tttactgaaa aaattaatga
    31501 aatgtagtac aaaacttta
//
LOCUS       DAEMHW010000029        30606 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEMHW010000029 DAEMHW010000000
VERSION     DAEMHW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.029"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..685
                     /locus_tag="KPK67_001095"
     CDS             <1..685
                     /locus_tag="KPK67_001095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458091.1"
                     /translation="LKHSYYKGTIYFGDIPTKVEAIQVDKSDEINKKGIKVESNENLK
                     EKIAIKQDEIILYKANFDKNINFYNYINIKFGDLRATGLDSQIQVNDNKNLYKNDTKF
                     KWHEGINRVKLSAKQINNKDIKINIQIKLSRNQYYYKELITENEHEFNVIKFINKNYD
                     LSSNINIREIVLNEFILNQNIEYSSGTEIWNLYSNKNIKHFSMRFDIVIPKYLNCNNK
                     NNFIILFGN"
     gene            769..1413
                     /locus_tag="KPK67_001096"
     CDS             769..1413
                     /locus_tag="KPK67_001096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458092.1"
                     /translation="MALINNLAVSNTTSEIDKQTCQVSVEGQVKIFSYKCCDAEDNIL
                     SKGKDYAFTLTYGNMGECPGTTQVTDCLSVTDIGGVQFKIMDVCGPCDNHKVYVGGCF
                     YYPGADLYFCSYPLVYSIGFIKLFNLEPVVCPESEICFYVFDGSSKDLSIAPHSINTF
                     GAILQAEDIFCEDCPCKACAPDAFNNSETIKPLDLDSSLSKEELENEFKKLLKL"
     gene            complement(1571..2386)
                     /locus_tag="KPK67_001097"
     CDS             complement(1571..2386)
                     /locus_tag="KPK67_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2458093.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYKYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASNGQGKGSEVLYYSN
                     KGLEYATRICDKLGTVFKNRGAKLDKSLYILNSSKPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKVLAQIISWNYKENECRVCN
                     IKDYIPGRTENLYIVGGGACNKISSITKEKYTMINGSDRFDTLYKALDFIDRL"
     gene            complement(2386..2817)
                     /locus_tag="KPK67_001098"
     CDS             complement(2386..2817)
                     /locus_tag="KPK67_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG2458094.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGGSK"
     gene            complement(2878..3015)
                     /locus_tag="KPK67_001099"
     CDS             complement(2878..3015)
                     /locus_tag="KPK67_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBG2458095.1"
                     /translation="MNWYKIITDFYKNNNWTKEQVKTAVEKKKITSTEYKEIVGEDYI
                     A"
     gene            complement(3017..3340)
                     /locus_tag="KPK67_001100"
     CDS             complement(3017..3340)
                     /locus_tag="KPK67_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458096.1"
                     /translation="MIFLGNLSNTDDINIKKVGLINYMPSDLSSKELEQGILVDNIMQ
                     EELREGYYSTLYVNELTKETYYKYKLIAKSGEELEKEILINKVNSTEQTIADLTFQLM
                     SNGVI"
     gene            complement(3341..4381)
                     /locus_tag="KPK67_001101"
     CDS             complement(3341..4381)
                     /locus_tag="KPK67_001101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458097.1"
                     /translation="MAKLNENSSLKEIMDTLENTTKEIEDNKVIYDNAITIVDKKANF
                     NDSTNVLNILYDDSYIYVLKASKLVKTDLNFNVIFSISYSNFKCFCISEDYIYASTLE
                     NIYQINKTTGTINKTIANNFVNDMCFYNNILYCYKSSTATMILINASSNLFSINLTKD
                     CASLSIKSFSSSGNIKVNSTGIYVLVQDSSDIASIYLLNHNLTQKIASATLRDSNAGR
                     KIMLLNNGVYVSCNRGVMGYPGEGYFNKYTSNLSLQIKLSDSKYGNLVGLDNNSNYIY
                     ATKGRREYEQAKIVKFSSNLNELNSYEFEGYGSPNVIYKNYNIYVNGNIDDKTIARLA
                     LTKKFYVKREMF"
     gene            complement(4394..4609)
                     /locus_tag="KPK67_001102"
     CDS             complement(4394..4609)
                     /locus_tag="KPK67_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458098.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDIENNFTSDNVEDALKELAIK
                     QNLLDTELNGQKTRGISIANTLTDMFL"
     gene            complement(4643..4825)
                     /locus_tag="KPK67_001103"
     CDS             complement(4643..4825)
                     /locus_tag="KPK67_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458099.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGAEPTV"
     gene            complement(4825..5118)
                     /locus_tag="KPK67_001104"
     CDS             complement(4825..5118)
                     /locus_tag="KPK67_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458100.1"
                     /translation="MNRANRIICDQTGKILLQTGEATGDILEHDEITELHFVDIPYGS
                     VDYTKSRIVGINIETKEPILEEIPVFVSEEEKRIQELENQLLIAENEKVGGIL"
     gene            complement(5132..6817)
                     /locus_tag="KPK67_001105"
     CDS             complement(5132..6817)
                     /locus_tag="KPK67_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458101.1"
                     /translation="MAKIYDFNRRYAEQTVTLPRGKYLLECWGAAGGGGSSIADSNLR
                     SKGGYSKGELTLKKETTLHVYVGYSGRDSLHSTPFNGGGKALIHESDSNKNTVYCHGG
                     GATDIRLVGGQWDNEQSLLSRIIVAGGGGRNNFGIKGGDGGGSNGGSTEYSEGGTNTG
                     GGKGINGTDGSFGKGGSGISSGEYSKQGGGGGGWYGGGSGGAAGGGSGYVLTENSYKP
                     AGYIPTKEYWLENTSMTVGGGIAGGDGKAKITLLQGLPFLVISSYTSEQAIFTAEHTE
                     ENKLSKIECFIDDVLKETFTTDLYLEKTINYTLEDNALHTLKIIVTDIDNMTYEQVVT
                     ISKSIMPLQTNANLQDISTKLVEIGESFKSGKTSIINTLALKNIEASLNNTLVELSEK
                     IKTSFDSSDASAQDLMNQLTQANNTISQLNSKYKYASGTIDVVKNSSLMANLYGESFG
                     RQPGTWLKIDNLGFIPNIFVAECQYVTSNNYFFKHIVIATCNINWFWDKKDFSARIVF
                     TKEKNSNQDFSGSGIIYSNNERDVYINNKGINVPASSPNVSSYLHSWHAIKFI"
     gene            complement(6831..7613)
                     /locus_tag="KPK67_001106"
     CDS             complement(6831..7613)
                     /locus_tag="KPK67_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG2458102.1"
                     /translation="MATDKSYYTILTDIGKAKIANASIIGEKVDFARIQLGDGGGNEY
                     NPTEEQTALKNVVWEGKVGNVKTDESMTNCLILESLIPASAGGFVVREIGYLDTEGNL
                     LAISKYRSAYKPKVEDGAVIDMKVKTIFVVSNVNNIELKIDPTIIFATLKDLQDLDTK
                     INTTKTELISNIETIKTELNNKIGDTTQLTTTDKTNIVSALNEVKTSVDSIETTAEKT
                     SYNNATSNLTATNVQEAIDEVVRKIENFNEININTINSILPI"
     gene            complement(7629..8246)
                     /locus_tag="KPK67_001107"
     CDS             complement(7629..8246)
                     /locus_tag="KPK67_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBG2458103.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSSIKPCNMTLDYTRVLVSKDNS
                     IYFGATTITGHEITIYPWSPSDIETQGEINILSGNGFGYQEITIY"
     gene            complement(8239..9288)
                     /locus_tag="KPK67_001108"
     CDS             complement(8239..9288)
                     /locus_tag="KPK67_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG2458104.1"
                     /translation="MFELMTFENIIKRMLDNVPDTFDKREGSIIYNALAPVAVELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATHTIAKGVFNIDIPLDSRFNLGE
                     YNYIATERVSEGIYKMKCETAGPIFELGQLIPIEYIEGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKLPDVGGVKVYPVWNGGGTVKLVIINSNFKV
                     PSSDLVNLVQEEIDPIGHQGQGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNL
                     TINSNSIVVRGEVVG"
     gene            complement(9289..9708)
                     /locus_tag="KPK67_001109"
     CDS             complement(9289..9708)
                     /locus_tag="KPK67_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG2458105.1"
                     /translation="MLPSDNLEYDIEDVSIINFDVRQEPSKTFKLNMERNKVDGICDD
                     VEALKQAIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENVDNFEFQNVKGKVHCKFTVYSKYGNIKAEKVVSV"
     gene            complement(9713..9973)
                     /locus_tag="KPK67_001110"
     CDS             complement(9713..9973)
                     /locus_tag="KPK67_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBG2458106.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESTSPLKVKIEQKL
                     SIGEFFLIQTDTFKKYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            complement(9987..11942)
                     /locus_tag="KPK67_001111"
     CDS             complement(9987..11942)
                     /locus_tag="KPK67_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG2458107.1"
                     /translation="MSDDLVLANDRDIRLVIAHWEDFYEPVVLDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRVEENKTLFDMVLTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSTTSKEDNDMINKLNKVFKNKLSNTGSIFVKYSNAYK
                     VNAALMAAISIHETGNGSSSLCKNKNNFFGMKGMSFGSVDEGIKRGISNLSRNYIHTG
                     RKTLESIRDKYAPLYDSPLNKDWVPGVGKFYKQITGSTYSSNNAGTGVGSNEEAEKNL
                     KDVTYQIQGNNQSSSTNNNSKADKLISVAKSKLGCNYVYGAEGPNTFDCSGFTQWCYK
                     QIGISIPRTVATQSKAGSAVDLKDKTKWKAGDLLCRVGGGSSNHVMMYIGNGQMIHSP
                     QTGDVVKIQSVDSYRKGKAYTHVRRFI"
     gene            complement(11935..12627)
                     /locus_tag="KPK67_001112"
     CDS             complement(11935..12627)
                     /locus_tag="KPK67_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG2458108.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTIDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFYQGTFKDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNMKVSLEEYNIVEDAENGSDTKVAIKLKQYRDYSTKKLVPAPPKTTETGRPSVKIEP
                     KRVDSINAPNTKTYTVKSGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKKLSK
                     YTIYKGQVLRLV"
     gene            complement(12644..14938)
                     /locus_tag="KPK67_001113"
     CDS             complement(12644..14938)
                     /locus_tag="KPK67_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBG2458109.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     AGNIQASQQSLNNIESILTRIEQKIGKADEEQRKFNEDINKGASSTNNLLGSVKKLAG
                     AYLGLKSIGGLVNLSDQMTSANARLNMINDGQLSDGGLNKMIFQSAERSRASYLDTAQ
                     IVSRIGMNAGSAFSSTREIVGFAEQLNKKFVIAGASTEEMNSALLQLTQGLGSGVLRG
                     EELNAVFESAPNIIHSIADYLDVDIGKIRGMASEGMLTADIVKNSLLAASAETNKQFE
                     QMPYTLGQIFTSVKNNAVMIFGAIQKKIEDTVSSGGFRTFIVNITDSLYILGSVGYKV
                     FNGIIDLLSSPAFSGFFNLMIVGVSLITQGLGWLLTTIGSVINFIAQGWSIIQPLLIT
                     SIVLWGLYKAAILAGVAITAIQVSWTALQAFWTNLLNGSLLTNIMMNIAAKVSTDALS
                     GSMLLLITTIVMVVATIALVIAAVFVGVAVFNHFAGTSVSAIGIVAGAISVAAAFIGN
                     LFVATGNLIIDIVALIYNSLANFAEFFANFLDNPIGSVIRAVSRMADAVLGILRSIAS
                     AIDTIFGSNLANAVSGWQDKLQGWTDKVAGEAKIKVERMDPNKLHFERFNYGKAWDAG
                     YKWGDKLETNIKDKFDISKIAEKAKKDLGLDDLWDKKYGLGDGFGSAGLNSPLGDAAK
                     GAKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQETINRFTGVNIKIDMNNTNNISKDAD
                     VDGIVNVLTEKLNDAMVVSAEGIV"
     gene            complement(15033..15602)
                     /locus_tag="KPK67_001114"
     CDS             complement(15033..15602)
                     /locus_tag="KPK67_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2458110.1"
                     /translation="MNDTVEYIFCSECGARCLKGSKFCNECGTKIDDVKIQKESITKD
                     KEYDLKGVDLQHIMQETSFIKASSVRRLKELTGIELDDCRKILEEPYQKYYNENADML
                     EEKRKKEDDISKIRKAKEEKKEKEKIACCPACGSTSLTAHKKDFGIGKAITGATIAGG
                     IGLVAGNIGAKKVRVTCLSCGKQFWAGKK"
     gene            complement(15645..16205)
                     /locus_tag="KPK67_001115"
     CDS             complement(15645..16205)
                     /locus_tag="KPK67_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458111.1"
                     /translation="MKKKVCFLFSILIVICLAIVGCSNSESPEESSKNNEPKKEEKKD
                     KEVVIGEKIISDKMEITINNIEFSYDVLPKVKESLYTHYPAESGKVYIDIAADIKNTQ
                     KQELNCSDLLTIEANYNDGYKYSSQTIVEDETTGFTYDNITSIDPLETKGVRFIIDCP
                     DEVKTSDKPLVLSFTFDGNKYVYKMK"
     repeat_region   16313..17131
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=16503..16531
                     /rpt_unit_seq="atttacattccatatagttaatctaaaac"
     gene            complement(17854..18015)
                     /locus_tag="KPK67_001116"
     CDS             complement(17854..18015)
                     /locus_tag="KPK67_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458112.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(18356..18796)
                     /locus_tag="KPK67_001117"
     CDS             complement(18356..18796)
                     /locus_tag="KPK67_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2458113.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(18868..19338)
                     /locus_tag="KPK67_001118"
     CDS             complement(18868..19338)
                     /locus_tag="KPK67_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG2458114.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            complement(19355..20665)
                     /locus_tag="KPK67_001119"
     CDS             complement(19355..20665)
                     /locus_tag="KPK67_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBG2458115.1"
                     /translation="MALGGGTFVTQNKILPGSYINFISAKRATSSLSDRGIVAIPLEL
                     DWGIDEDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIAIAKYSGIRGNDLKIIVTTNIDDNTKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGFVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSIENKIKDKDLVESSLIYWATGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVN"
     gene            complement(20667..20843)
                     /locus_tag="KPK67_001120"
     CDS             complement(20667..20843)
                     /locus_tag="KPK67_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458116.1"
                     /translation="MSKTLSKGTDYKFTKEQIVNSKKYINRKDLLNAILKENELYSFS
                     EVEEIINSFMKGVS"
     gene            complement(20836..21273)
                     /locus_tag="KPK67_001121"
     CDS             complement(20836..21273)
                     /locus_tag="KPK67_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458117.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYPSGRTLKKNSFDVHYFPKSNDKSFEINEVAEMLLEELEYIEIDGDLVRGTNMN
                     FEIVDNVLHFFVDYNYFTIKNNDINKMDTVELFGGLKRGDNFE"
     gene            complement(21266..21691)
                     /locus_tag="KPK67_001122"
     CDS             complement(21266..21691)
                     /locus_tag="KPK67_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG2458118.1"
                     /translation="MAIWGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRIPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQIDKIIEKKLLILLKGVFDA"
     gene            complement(21691..22038)
                     /locus_tag="KPK67_001123"
     CDS             complement(21691..22038)
                     /locus_tag="KPK67_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458119.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(22032..22433)
                     /locus_tag="KPK67_001124"
     CDS             complement(22032..22433)
                     /locus_tag="KPK67_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458120.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            complement(22443..22676)
                     /locus_tag="KPK67_001125"
     CDS             complement(22443..22676)
                     /locus_tag="KPK67_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458121.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(22695..23585)
                     /locus_tag="KPK67_001126"
     CDS             complement(22695..23585)
                     /locus_tag="KPK67_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBG2458122.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEA"
     gene            complement(23597..24160)
                     /locus_tag="KPK67_001127"
     CDS             complement(23597..24160)
                     /locus_tag="KPK67_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG2458123.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(24226..24426)
                     /locus_tag="KPK67_001128"
     CDS             complement(24226..24426)
                     /locus_tag="KPK67_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458124.1"
                     /translation="MKLWEYVGKNVQITCVDKQIIRGKCDGYTQALDNEPEIASISIA
                     RDGYGIEVYENEIESIENINKE"
     gene            complement(24441..25838)
                     /locus_tag="KPK67_001129"
     CDS             complement(24441..25838)
                     /locus_tag="KPK67_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBG2458125.1"
                     /translation="MKYKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAINSIEKDI
                     LSWYNRFTESEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKYRPTLVNELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLDVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKYVKHNSILEIKNSAIIDSIKEDIKNGKYNLNIHDGKQGKHLKEHNNYIEGRSY
                     LTITKEEAQELVNKHAGNGIIKFNRSGEWDKKELIEVDKNIGVNVNNITGEKTLTNKF
                     KIHYSKTGTHIVPAL"
     gene            complement(25828..27267)
                     /locus_tag="KPK67_001130"
     CDS             complement(25828..27267)
                     /locus_tag="KPK67_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG2458126.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEIQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDE
                     LERLKKQEDTQKEYDDLIPNNQDGVIDEI"
     gene            complement(27273..28526)
                     /locus_tag="KPK67_001131"
                     /pseudo
     CDS             complement(27273..28526)
                     /locus_tag="KPK67_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
     gene            complement(28504..29196)
                     /locus_tag="KPK67_001132"
     CDS             complement(28504..29196)
                     /locus_tag="KPK67_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458127.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKGKEEMVKELKKYESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKNRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
     gene            complement(29266..29775)
                     /locus_tag="KPK67_001133"
     CDS             complement(29266..29775)
                     /locus_tag="KPK67_001133"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458128.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            complement(29796..>30606)
                     /locus_tag="KPK67_001134"
     CDS             complement(29796..>30606)
                     /locus_tag="KPK67_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458129.1"
                     /translation="KDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEG
                     NKKMDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKF
                     DNNMSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRH
                     NDLIYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIK
                     LCREIEVSLDIDNHIDSEDIFDKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
BASE COUNT         9305 a         5701 c         3286 g        12314 t
ORIGIN      
        1 tttaaaacat agctattata aaggaacaat ttatttcggt gatatcccaa ctaaagttga
       61 agcaatccaa gtagataagt ctgatgaaat aaataaaaaa ggcataaaag tagaatccaa
      121 cgaaaactta aaagaaaaaa tagctataaa acaggatgaa attattctat ataaagcaaa
      181 ttttgataag aatataaatt tttataatta tataaatatt aaatttggag atttgagagc
      241 cacaggatta gattcgcaaa tacaagttaa tgataataaa aatttgtata aaaatgacac
      301 taaatttaag tggcatgaag gtataaacag agttaaactt tcagctaaac agataaataa
      361 taaagatata aaaattaata tacagataaa actatcaaga aatcaatatt attataaaga
      421 attgattaca gaaaatgaac atgaatttaa tgtaattaaa tttattaata aaaattatga
      481 tttaagtagt aatataaata taagagaaat agtattaaat gaatttattt taaatcaaaa
      541 tatagaatat tcttctggaa ctgaaatatg gaatttatac agtaataaaa atattaagca
      601 tttttcaatg aggtttgata tagtaattcc aaaatattta aattgtaata ataaaaataa
      661 ttttataata ttatttggaa attagggtca aaacacttta aatttcatat tatatattaa
      721 gcttatctta acaatagcta agctaatata aatataggag tgtttaaatt ggcattaata
      781 aataatttag cagtatctaa tactacaagc gaaatagata aacaaacttg tcaagttagt
      841 gtagaaggac aagttaaaat attttcttac aaatgctgtg atgcagaaga taatatatta
      901 tcaaaaggta aagattatgc atttacatta acgtatggta atatgggaga atgtccagga
      961 acaacacaag taactgattg cttatcagta acagacatag gtggagttca atttaaaata
     1021 atggatgttt gtggaccttg tgataatcat aaggtttatg taggcggatg tttctattat
     1081 cctggagcag atttatattt ctgttcatat ccattggttt attcaatagg attcattaaa
     1141 ttatttaacc ttgaacctgt agtttgtcca gaatcagaaa tatgtttcta tgtttttgat
     1201 ggttcaagca aggacttaag tattgcacca catagtataa atacttttgg tgcaatatta
     1261 caagcagaag acatattctg tgaagattgc ccatgtaaag catgtgcacc tgatgcattt
     1321 aataattcag aaacaattaa acctttagat ttagatagtt cattatcaaa agaagaatta
     1381 gaaaatgaat ttaagaaatt actaaaatta taatactcag taaagtgaat aacatcaaat
     1441 aaaattttca taaaattatt tactattaga taagaatata gctactttaa attttatgta
     1501 aacaaaatta gtataaagtt taaattttaa aaggcgttaa ccatactagg ttttcgcctt
     1561 tatatttagt ttacaaccta tcaataaaat ctaatgcttt ataaagtgta tcaaatctat
     1621 cactaccatt tatcatagta tatttttctt tagtaataga acttatctta ttacatgctc
     1681 ctccacctac gatatataga ttttctgttc ttcccggaat gtaatccttt atattgcata
     1741 ctctacattc attttcttta taattccaac taattatttg tgctagtact ttgtcaactt
     1801 ctccatcata aacaattgta tgtttgtaca tctgtttaac tccctcatta tttatatttt
     1861 tatttaatac accttctaca attaacttag caataccttc atgacctagt ttcttagctt
     1921 tctcataatc ttctttgtta tcgcaaaaga aactttcaat taatactgct gtaggttttg
     1981 agctatttaa gatatataaa cttttatcta atttagcacc tctattttta aatactgtac
     2041 ctagtttatc acatattcta gttgcatact ctaaaccttt attactataa tacagtactt
     2101 ctgagccttt accttgaccg ttacttgcat ttaaatgtag ctctataagt aagtcatatc
     2161 ctccactatt aactctaggt attttataag tcttttcttc tgctttagtt ttaaactgct
     2221 tttcagggca tattattaca tctgccttat gaccttcttt tctaaatgta tctgctaata
     2281 ctggggcaag agatttattg tacttatact cgttaactac tccatccgca gaagtacatg
     2341 ctccactttt taaaatactg tgtcctactg taatacatat tttcattatt tactacctcc
     2401 tttaacattt aattcatctg tcatagtatc taataaacta cctattttct cttttaatcg
     2461 tttaggaact ggtaatccac ataagtacat attttttaat atacttacac tttcatatag
     2521 aataaataaa atagagaaaa attcagatat tccaagatga tttaatcgca aaaaatcaac
     2581 ccaatcttgt ggcaacataa ataaaaagtt aaactttgta agaatgtcaa ctactgctag
     2641 aaaaaatata catgctatca ttgctacttt tcttattcct ccatttattc caaaagagct
     2701 attaaactga tgtgttttta ttgctcttaa gcaacctaac agtgtatcaa atgctattgc
     2761 taatattact aattttataa atatatttgt tgctaaaaaa actattgtta cgttcatatt
     2821 tcctccttat tctgcattaa aataagactt agaaattatc taagtctatt aaaaaaacta
     2881 cgctatatag tcctctccta caatttcttt atattctgtt gatgttatct tcttcttttc
     2941 tactgccgtt ttaacttgct ctttagtcca gttattattc ttatagaaat ctgttattat
     3001 cttataccaa ttcatactat atcaccccat tactcattaa ttgaaatgtt aaatctgcta
     3061 ttgtttgctc tgtagaattt actttattaa ttaagatttc tttttcaagt tcttccccac
     3121 tttttgctat taatttatat ttgtagtatg tttcttttgt taattcattt acatataaag
     3181 tagagtaata tccttctcta agttcttctt gcataatatt atctaccagt attccttgtt
     3241 ctaattcctt actagacaaa tcagaaggca tgtagtttat caagcctact ttttttatat
     3301 ttatatcatc tgtattactt aaattaccta aaaaaatcaa ttaaaacatc tcccttttca
     3361 catagaattt ttttgttaat gccaaccttg ctatagtttt atcatcaata ttaccattca
     3421 cataaatatt ataattttta taaattacat ttggagaacc atatccctca aattcatatg
     3481 agtttagttc atttaaattg cttgaaaact ttactatttt tgcttgttca tattcacgtc
     3541 gaccctttgt tgcataaata taattactat tattatctaa tcccactaag tttccatatt
     3601 tgctgtcaga caattttatt tgtaaactta agtttgaagt atatttatta aaataacctt
     3661 ctcctggata acccataact cccctattac atgatacata aactccatta tttaacaaca
     3721 taattttacg tcctgcatta ctatcacgta aagttgctga tgcaattttc tgagttagat
     3781 tatgatttaa caaatatatg gatgctatgt cagaactatc ttgtactaat acatatatac
     3841 ctgtactatt tacttttata tttccacttg atgaaaaact ttttatagaa agagaagcac
     3901 aatctttagt taagtttata ctaaataaat ttgaagaagc attaattagt atcatagttg
     3961 ctgtactact cttataacaa tataaaatat tattataaaa acacatatca ttaacaaaat
     4021 tattagctat agttttatta atagttccag tagttttatt aatttgatat atattttcta
     4081 atgtagaagc ataaatataa tcttcactaa tacagaaaca tttaaaatta gaataactta
     4141 ttgagaaaat aacattaaaa tttaaatctg tttttactaa ttttgatgct tttaatacat
     4201 agatataact atcatcataa agaatgttta aaacatttgt tgagtcatta aaatttgctt
     4261 ttttatcaac tattgtaatt gcattatcat atatgacttt attatcctct atttcttttg
     4321 tagtattttc aagagtatcc attatttctt tcaaactact attttcattt aactttgcca
     4381 ttattaattc tccttataaa aacatatctg ttaatgtatt tgctattgat attcctcttg
     4441 tcttttgacc atttaattct gtatctaata aattttgctt tattgctaat tcttttaaag
     4501 catcttctac attgtcactt gtaaaattat tttctatatc ttctatagtt acattctttg
     4561 cttctaatac aagatttcta actttattaa ctaactcttt aaaagtcatt tagtcacctt
     4621 ctttcaataa aaaaagaacc ttctatacag ttggttctgc tccttctact actccactat
     4681 tttttataat ataatcctct acagcttttc tgtactctgt gttagtcacg tcatctaact
     4741 gaaattctct atttttcaat gggtttaacc ctccgtttaa tattctctct gctaatattc
     4801 ttactacaac attatttata ttcattataa aattcctcct actttttcat tttctgcaat
     4861 tagtaattga ttttctaact cttgtattct cttttcttct tctgaaacaa atactggtat
     4921 ttcttctaag ataggttctt ttgtttctat atttatgcct acaattctgc ttttagtata
     4981 atctacactt ccatacggaa tatcaacaaa atgtaattcc gttatttcat catgttctaa
     5041 tatatcccct gttgcttctc cagtttggag taatattttc ccggtttggt cacatataat
     5101 tctatttgct ctattcattt tatcacctca tttatataaa ttttatagca tgccaagaat
     5161 gtaggtaact agaaacatta ggactacttg caggaacatt aatgccttta ttatttatat
     5221 atacatcacg ctcattattt gaataaataa ttccacttcc actaaaatct tgattagaat
     5281 ttttttcttt agtaaataca attctagctg aaaaatcttt tttatcccaa aaccaattta
     5341 tattacaagt agcaataaca atatgtttaa aaaaataatt attagaagta acatattggc
     5401 attcagcaac aaaaatatta ggaataaaac ctaaattatc aatcttaagc caagtaccag
     5461 gttgtctacc aaatgactct ccatacaaat tagccattaa agaactattt ttaacaacat
     5521 caatagtacc actggcatat ttatacttag aatttaactg tgatatagta ttattagctt
     5581 gtgttaactg attcatcaaa tcctgcgcac tagcgtctga actatcaaaa cttgttttta
     5641 ttttctcaga taattccacc agtgtgttat tcaaacttgc ctctatattc tttaatgcta
     5701 aagtatttat aatacttgtt ttaccacttt taaaactttc tcctatctct actaacttag
     5761 ttgatatatc ttgtaaatta gcattagtct gtagtggcat tatactttta cttatagtaa
     5821 caacttgctc ataagtcatg ttatcaatat ctgtaacaat tattttaagt gtgtgtagtg
     5881 cattatcttc taatgtatag ttgattgttt tttctaagta taaatctgta gtaaaagttt
     5941 cttttaatac atcatctata aaacattcaa ttttagatag tttattttct tctgtatgtt
     6001 ctgctgtaaa aatagcctgt tctgatgtat aagaactaat aacgagaaaa ggcaaacctt
     6061 gcaatagtgt tattttagct tttccatctc ctcctgcaat acctccacca acagtcatag
     6121 aagtattttc tagccaatat tctttggttg gtatatatcc agctggttta tagctatttt
     6181 ctgttaaaac atagccactt ccaccaccag cagcaccacc acttcctcca ccataccatc
     6241 ctcctccacc tcctccttgt tttgaatatt caccactgga tattccgctt cctcctttcc
     6301 caaatgaacc atcagttccg tttatgcctt tccctccacc tgtatttgtt cctccctcgc
     6361 tgtattcggt agaaccaccg ttgctaccac caccatctcc accttttatt ccaaaattat
     6421 ttctaccacc tccacctgct acaattattc tagataaaag actttgctca ttatcccatt
     6481 gccctccaac gagtctaata tcagtcgctc ctcctccatg gcaatagacg gtatttttat
     6541 tactgtcact ttcgtgtatt agcgcctttc ctcctccatt aaacggggtt gaatgcagac
     6601 tgtctcgacc actgtatcca acatatacat gcaaggtagt ttcttttttt aatgtaagtt
     6661 cgcctttact ataccctcct ttagaacgta aattagagtc agctatgcta ctgcctccac
     6721 cacctgctgc tccccaacat tcaagtaagt acttgccacg tggtaaagta acagtttgtt
     6781 cagcatatct acggttaaag tcataaatct tagccatttc attctccttt ctatataggt
     6841 aatatactat ttattgtatt tatattaatc tcattaaaat tttctatctt tctaacaact
     6901 tcatcaatcg cctcttgcac attcgtagca gtaaggttgc ttgttgcatt attataactt
     6961 gttttctctg ctgttgtttc tatactatct acactagttt ttacctcatt taatgcacta
     7021 actatatttg ttttatctgt tgtagtaagt tgtgttgtat ctcctatttt gttgtttaac
     7081 tctgttttaa tagtttctat attacttatt aattctgttt tagttgtatt tattttagtg
     7141 tctaggtcct gtaaatcttt taatgtggca aaaattatag ttgggtcaat tttaagttct
     7201 atgttattta cattagatac aacaaagata gtttttacct tcatgtcaat tactgctcca
     7261 tcttccactt taggtttata tgctgaccta tactttgata tagctagtaa attaccttcg
     7321 gtatctaaat aacctatctc tctaactaca aacccaccag cactagcagg tattaaactt
     7381 tctaaaatta agcaatttgt catgctttca tcagtcttga cattccctac tttaccttcc
     7441 caaactacat tttttaatgc tgtttgttcc tctgtaggat tatactcatt tcctccacca
     7501 tcacctaatt gtatcctagc aaagtcaact ttttccccta ttatacttgc atttgctatc
     7561 ttagccttac ctatatctgt taatatcgtg tagtaacttt tatctgtagc caaactacca
     7621 cctcctatct aataaattgt aatctcttgg tatccaaatc cattaccact aagtatatta
     7681 atttctcctt gcgtttctat atcactaggt gaccaagggt aaattgtaat ttcatgtcct
     7741 gttattgtag ttgcaccaaa atatatacta ttatctttac ttacaagcac tctagtgtaa
     7801 tctaaagtca tgttgcaagg tttaatacta cttacaaaag aatgaacctc ctcaaaccaa
     7861 tcttgatttc tagcatcact ttcaagatgt atattataag tagcattatt tatagttaac
     7921 tcataattac cttctccaac tatactatct agccaattcc ttaaaaatct ctctgagtaa
     7981 ggtaatttac ttatatattt actaaaaatc ctaaaccttc tatcttctaa actctcatta
     8041 cttttaggag ttatagacat tatcttttcc catcttttta taccacttat agttaggtcc
     8101 tctaaaaact ggtcatttga taggtccttt aatttatcat gtagtgtttt tatttcttta
     8161 ttttctacat taaatacttt tatatattct tctttatctt gtagaatttg tggcaagtaa
     8221 tttattagat taatctcttt atccaactac ctcacctctc actactatac tgttactatt
     8281 tattgttaga ttagatttaa cctcatttat cattgtattt gcaatgtcta atactccatc
     8341 aatactaagt aatctagttt caatttgaga tatacggact attaagtttt cttcatcttc
     8401 ccaactcatg ttaagttcat ttaaatagtc gtctattgct tcttctgcaa ttgattttat
     8461 attctcccaa gtgtagccat ttttgtatgt tatctctgct gatatattta tagttgtact
     8521 tgtaactcct tcaactgtga ctcggtgtcc aattggtgct aatcctaagc cttgtccttg
     8581 atgtccaatt gggtcaattt cttcttgaac taaattaact aaatcactag atggaacttt
     8641 gaagttagaa ttaattatta ctaacttaac agtacctcca ccgttccaaa ctggatatac
     8701 tttaactccc ccaacgtcag gcaatttatt aacttcatcc ttataattct gtatattacc
     8761 accgaaactt tgtgagttta aactatcata gtatctttgt cttaaactgt cctcagattc
     8821 ttcatcttct ccatttatta gtatttctgt taactctgct gtttcaagac cctctatata
     8881 ttcaattgga atcaactgac caagttcaaa aataggtcca gcagtctcac atttcatctt
     8941 atatatacct tcgctaaccc tttcagttgc tatataattg tactctccta gattaaacct
     9001 agaatcaagt ggaatatcta tattgaaaac gcctttagca atcgtatgag tggcttcaag
     9061 tggtgttatt cccctttctt tacatctctt ctctaaataa taataactag cagtatctac
     9121 aaaagtttgg tctagtaatt catccattgc aatgtatgtt tctgtaagct ctactgcaac
     9181 aggagcaaga gcattgtata ttatagaacc ttctctttta tcaaaagtat ctggtacatt
     9241 atctaacatt cttttaatta tattttcaaa tgtcattaac tcaaacaatt atacactcac
     9301 caccttctct gcttttatat ttccatattt actgtaaact gtgaatttac aatgtacttt
     9361 accctttaca ttttgaaact caaaattatc tacattttca atcctatcat cttgaattag
     9421 tgcttctgtt atccttcttt caagttcggg tattacatat gaaataggtt ctccaattaa
     9481 atcatttaat tcaacaccat aatttctaga ataaataagg tgctcatacc tttcagtatt
     9541 taaaattaaa aagatggctt gttttaatgc ttccacatca tcacaaatac catctacttt
     9601 atttctctcc atattcaatt taaacgtctt acttggttct tgccttacgt caaaattaat
     9661 tattgataca tcttcaatat catactctaa attatcgctt ggtaacacct catcacatcc
     9721 tatctaaaat caagtattgt tgccctcctt gcatcctaat taagactaat ttatctccta
     9781 tttttttatc tgtatatttt ttgaatgtat ctgtttgtat tagaaaaaat tcaccaatag
     9841 atagtttttg ctctatttta acttttagag ggctagtact ttctattgtt ccaaatacaa
     9901 ccctcattgg gttgcttgtt tctactgcat ccattgcagc tttttttatt atctgtaata
     9961 aatcttggct catattatca cctcacttat ataaatcttc tcacatgtgt atatgctttt
    10021 ccttttctat atgaatcaac tgattgtatt tttactacat caccagtctg tggtgaatga
    10081 atcatttgac catttccaat atacatcatt acgtgattac tgcttcctcc accaactcta
    10141 cacaataggt ctcctgcttt ccatttggtc ttatctttta aatctactgc actacctgcc
    10201 ttgctttgtg ttgcaactgt tctagggata cttatgccta tttgtttata acaccactgt
    10261 gtaaatccag aacaatcaaa tgtattgggt ccttctgctc catacacata attacaaccc
    10321 agtttacttt ttgctacact aattagttta tctgctttag aattgttatt tgtactactg
    10381 ctttggttgt taccttgaat ttgataagtt acatctttta aattcttttc tgcttcctcg
    10441 ttgcttccaa ctcctgtacc tgcattatta gaactatagg tacttcctgt tatttgttta
    10501 taaaatttgc ccactcctgg tacccaatct ttattaagag ggctatcata aagaggtgca
    10561 tatttatctc ttatactttc taatgttttc cttcctgtat ggatataatt tcttgataaa
    10621 ttacttatac ctctttttat tccttcatct acagagccaa aagacattcc tttcattcca
    10681 aagaaattat ttttattttt acaaagtgaa gaacttccat taccagtttc atgtatagaa
    10741 atagcagcca ttaaagctgc attgactttg taagcattag aatatttaac aaatatactt
    10801 cctgtatttg ataacttatt tttaaatact ttatttaatt tatttatcat atcattgtct
    10861 tctttacttg tagtactttg tgcaggacca tttttctttt catctttatt attagtattt
    10921 ccactactat atgagcttga agaataagaa gcaaattcat ctccatcaac aagggtcaaa
    10981 tccataaagt gcgaattatt ttcaaatgta tgttttactt tctcaactaa catataattt
    11041 tgtaattcaa tatctcctag atttaaaaaa acaggtacta aacaacctgc tcttactcta
    11101 atatcaccaa gtgcattttt taaacttaat gacttagttt tcttattata tagttttaga
    11161 agtatatcac acttttgttt tatctctgct tcactcatgt ttttgtctac tgtatcaaac
    11221 atttgaagta ttccccaact cctcatatgt gtagagtctt gtgcaatata tacatctctt
    11281 ttccctgttt cttcattatc tctcacaagt ttgatttttg tataagtatc actatcaata
    11341 gaagaattgt agtcaaagtc ctctatgaca tcattattca taacagtatc taatttcatt
    11401 gatgcaacat tctttaatgt tattcttcca aaatcatcat ataaaacata catttctttt
    11461 ttctctctta gagtatcatc tagtgcagtt aaaaccatat caaagagtgt tttattttct
    11521 tcaactctag atattttata ttttgtatct tctatgacat tgtattttaa attaaaatcc
    11581 ttagccaaca tctttacaag ttcacttgct gttttattac tatatacata agtatcttta
    11641 ttcttaaaat atcttagctg gtcataagca actatcttga tatgattttc tttatctctc
    11701 tttttctgaa atatatatcc atagaatata cctacacctt tgtaatatag tcttacagag
    11761 ttaccttcac aaaactctaa tatatcatcc atgactattg taaattcaag tttagatggt
    11821 gttcctcgtc tctctatttc ccatgtgata ccatccaaaa ctacaggttc gtagaaatct
    11881 tcccaatgtg caataactag ccttatatct ctatcattcg ccagaactaa atcatcagac
    11941 aagcctcaac acctgccctt tatagatggt gtatttactt aatttttttc ccttgttagc
    12001 tttatccatc atcgatttat ttaattcata tactttctta tataatgaac catttcctaa
    12061 ctgtttctga caaattgacc aaagagaatc acccgatttg actgtataag tcttggtgtt
    12121 tggtgcatta attgaatcga ccctcttggg ttctatcttt acgcttggtc taccagtctc
    12181 agttgtttta ggcggagcag gaactaattt tttagttgag taatctctat attgctttaa
    12241 ctttattgca acttttgtat ctgagccatt ttctgcatct tctactatgt tatattcctc
    12301 aagagatact ttcatattag tgttaaatag tactttatta cctaattccc tcgatacaat
    12361 aaattgaaat ggcttacaat cagtttttag taattctagt ttacttaaaa agaattgaac
    12421 atctttaaaa gtcccttgat aaaatggtag cttattgtgt gtaaattctg cttcaaaact
    12481 tatctcagat agcccttctt tttttaatat atttacttct ccaacattta ttaaatcaat
    12541 tgtcttattt ttatttgtca ctttgacttc aagcttgggt ggtgcgattg gtagttgtac
    12601 tccatctagg taaaaatcat aagccatttt ctcactctcc tttctaaact attccttccg
    12661 ctgatacaac catagcatcg ttcagttttt cagttaagac attaactatt ccatcaacat
    12721 ctgcatcttt acttatgttg tttgtattgt tcatgtcaat tttaatgttg actcctgtaa
    12781 aacggtttat tgtttcctgt tctgcaatat ctctaagata ttttaagtct tcttggcttt
    12841 tatccattgt tttagccatt ttagctgtat ttcccgcagt gtcttttgct ccttttgctg
    12901 catcgcccaa aggtgagttt aatccagctg aaccgaatcc gtcaccaagt ccatattttt
    12961 tatcccataa atcgtctaat cctaaatctt tttttgcttt ttcggctatt ttgctaatat
    13021 caaatttatc ttttatgtta gtttctagct tatcgcccca cttatatcct gcatcccatg
    13081 cttttccata attaaatcgt tcgaaatgta gtttattagg gtccattcgt tcaactttta
    13141 tcttagcttc tcctgctact ttgtcagtcc acccttgtaa tttatcttgc caaccactca
    13201 ctgcatttgc tagatttgag ccaaatatgg tatctattgc agatgctatg cttcttaata
    13261 tacctaatac cgcatcagcc attctagata ccgcccttat gacagagcca atcgggttgt
    13321 ctaaaaagtt agcaaagaac tctgcaaagt ttgctaaact attataaatt aaagctacaa
    13381 tatctataat taagttccct gttgcaacga ataagtttcc tataaaagca gctgcaactg
    13441 atattgcacc tgcaactata cctatagcag atacactagt ccctgcgaaa tgattaaaaa
    13501 ctgctactcc tacgaaaact gctgctatta ctagagctat tgtagctaca accattacta
    13561 tagtagtaat tagaagtaac atagaaccac ttaaagcatc tgtagatact tttgctgcta
    13621 tattcatcat tatatttgtt aataatgaac catttaataa attagtccaa aatgcttgca
    13681 gagctgtcca actaacttga atagcagtaa ttgcaacacc tgctaaaata gcagctttgt
    13741 ataaccccca caatacaata ctagtgatta ataaaggctg tattatgctc catccctgtg
    13801 caataaaatt aatgacactt cctatagttg ttaatagcca tcctagtcct tgcgtaatta
    13861 agctaactcc aacaatcatc agattaaaaa atccactaaa tgccggacta cttaataaat
    13921 cgattattcc attgaacact ttataaccaa ctgagcctaa tatatatagc gaatctgtta
    13981 tattaactat gaaggttcgg aatcctccac ttgagactgt atcctcaatc tttttttgta
    14041 tcgctccaaa aatcataact gcattatttt ttacactagt gaaaatttga ccaagtgtat
    14101 aaggcatctg ttcaaattgc ttatttgttt cagctgatgc agcaagcaag gaatttttta
    14161 caatatctgc tgttaacatt ccttcacttg ccatacctcg aatttttcct atgtcgacat
    14221 cgagataatc ggcaatcgag tgaataatgt taggtgctga ctcaaataca gcatttaatt
    14281 cttcacctct cagtacccct gaacccaaac cttgggttag ctgtaacaac gccgagttca
    14341 tttcctcagt acttgcgcct gctattacaa attttttgtt taattgctct gcaaaaccta
    14401 caatttctct agtactagaa aatgcacttc ctgcattcat acctatcctt gatactatct
    14461 gtgctgtgtc taggtaagat gcacgagacc tctcagctga ttgaaaaatc attttattta
    14521 atcctccatc agataattga ccatcattta tcatgttcaa tctcgcattt gcgctggtca
    14581 tttggtcact taaatttact aaccctccaa tactttttaa tcctaaataa gctcctgcta
    14641 gtttcttaac acttccaagt aaattatttg tagaacttgc tcctttattg atatcctcat
    14701 taaactttct ttgttcttca tctgcttttc caattttctg ttctatcctt gtgagaatac
    14761 tttctatgtt attcaaactt tgttgagaag cttgtatatt acctgcattg agtggattat
    14821 tcaatctgcc ttgcaatctc tctaaactat taattgttgt attaatagaa ttagtcatat
    14881 tacgaaaagc gggtgtcatt ccgtcaaaaa tttttattga tgtctgtatc gtagccattt
    14941 cctcactctc ctttcttatt attttaaaca taataaaaag cactaactaa tttagtaagc
    15001 gcttcctttt ctttattata atcttaatat atctattttt tacctgccca aaattgcttg
    15061 ccacagctca agcatgtaac tctaactttc tttgctccta tgtttcctgc tacgagacca
    15121 atgcctcctg ctatagtagc tcctgttata gcttttccta tgccaaaatc ttttttatgt
    15181 gctgtcaaag aagttgagcc acatgcaggg caacaagcta ttttttcttt ttctttcttt
    15241 tcctctttgg cttttctaat ttttgatata tcatcttctt tttttctttt ttcttcaagc
    15301 atatcagcat tttcattata atatttttga tatggttctt ctaatatttt tctacaatca
    15361 tccaactcta ttccagttaa ttctttcaat cttcttacag atgaagcttt tataaaactt
    15421 gtttcttgca ttatatgttg taaatcaaca ccttttaaat catattcctt atctttagta
    15481 atgctttctt tttgtatttt tacatcatct atttttgttc cacattcatt gcaaaatttg
    15541 cttcctttta aacaccttgc tccacattcg ctacaaaata tatattctac tgtatcattc
    15601 ataaaatatc tccttagcac tataatatat tcattttaaa taagttactt cattttataa
    15661 acatacttat tcccatcaaa tgtaaaactt aataccagcg gcttatcact tgtttttact
    15721 tcatccgggc aatcaattat aaatctaact cccttcgttt ctaaagggtc gatactggta
    15781 atattatcat aagtaaaacc tgttgtttca tcttctacta ttgtttgtga tgaatattta
    15841 tacccatcat tataatttgc ttcaatagtt agtaaatctg aacaatttaa ctcttgtttt
    15901 tgagtatttt ttatatcagc agcaatatca atatatactt tgcctgactc agcaggataa
    15961 tgtgtatata aactttcctt aacttttggt aaaacatcat aagagaattc aatattatta
    16021 atagtgattt ccattttatc tgaaataatt ttctctccta tgactacttc tttatccttt
    16081 ttttcttctt tttttggttc attattttta ctactctcct ctggactttc tgaattagaa
    16141 catcctacaa tagctaaaca gataactata agaatagaaa ataaaaaaca tacttttttc
    16201 ttcataatat aatatccccc taaattatat tctttaacaa tattatacta tattagtaaa
    16261 atttttacat tataatcacc tactttctaa gtaagtgttt ttagcgtttt taattttaag
    16321 tccacatagt taatataaaa tgtacttatt taattgcatt aggaagtata ttgcttattt
    16381 atataccaca tagttaatat aaaatttttt tgaaattctt gcatttgagg taattccatt
    16441 ccatttacat accacatagt taatctaaaa caagtactta aaaatgttac agcatcagga
    16501 ccatttacat tccatatagt taatctaaaa cccaagccaa accacaaaag tattaactat
    16561 ttctcgaaca tttacattcc atatagttaa tctaaaacaa ctccgcattc taaaattaat
    16621 gtctcatttt ctagtattta cataccactt agttaatata aaacactgat gattttctaa
    16681 agaaacatcc agtttttgaa taatttacat tccatatagt taatctaaaa cttggagaaa
    16741 ataatcctcc ttatgattta ccaagcggat ttacattcca tatagttaat ctaaaacaag
    16801 aagattattt tactttttca gatgaagctt taggatttac attccatata gttaatctaa
    16861 aacatttttt tacaggagta ttataatatg aatgatttaa gatttacatt ccatatagtt
    16921 aatctaaaac agtgcactaa gtatgacagc ttttggtact gctgtattat ttacattcca
    16981 tatagttaat ctaaaacaag tagaaagaca gattatttaa cttattcatt aattgaattt
    17041 acattccata tagttaatct aaaacctata acaggagttg cagaaaaggc attaaaaggt
    17101 caatttacat tccatatagt taatctaaaa ccccaaaata aacttagcat ttccaatacc
    17161 tacatatata caatactctt aaaattgcag tgagcgggca atagtgcaat tgataacact
    17221 tatcacgcac cctcaacatc ttagatttca agtgttaagc catattttgt aacaaatatc
    17281 gctcactgca aaatctctac atttttatta tatcataaaa atattatttt tgaatatctg
    17341 taccaatttg tggtataata aaagcaagga aataatttac tcctacaaag agtgattatt
    17401 ttcagttgtt aagtgaaaat attatttttt taaatcaccc ttattggcgt ctgggtgatt
    17461 ttttattttg tcataaatat aagctgatat aacaccagct agtatgctta ataaaaaacc
    17521 tatcatataa tttcacctcc ttccttattt ggaatttggc gtttaatatg aaaataatca
    17581 cccttcgcac tttcgattat tatccttgct acaattatta ttacatattt ttccattttt
    17641 tttatataaa caatgaaatt caagtaaata aatacctact tatttatata tattttataa
    17701 attaattgct ttataatcaa gttttcaatt ttttaataaa aatttttatt ttttatttta
    17761 tataaacaat attttttcta atttgtggta taataaaagc aagaagaact acaatctatt
    17821 ttgcggtaga gtgaagttca taattaaatg aatctatttg aacttatgga acttgatttt
    17881 aaaatcaaat tcccagccac ttttactctt gccacgagtt gagtggcttt ttacttttag
    17941 aaatacttta caaattaagc aaaatattaa actagcaata acgccagcta ttacattaag
    18001 taaaaagtta ttcatacttc ccacctcctt tcattaggaa gtaggtttta tcccagtatg
    18061 aactccactc tataaattgt agattacatc ttcttgctaa aaatattata acatataatt
    18121 attacatatt ttacctattc tatatttatt tttttatttt gctatcttct tcgtcccctc
    18181 tttctctctc tttcagcttc tttcattgct tcctcttcat cctctatctt tataagtatt
    18241 gaggcggctg ctaacgctct ctcattaact tctaaattca tatattcact tggcttccac
    18301 tttaattttt gaatacaata atgagtgatg ctagcatcaa aatcgccacc tctgattagt
    18361 tttttgcttc ttctacttta tcctcaaaag atgtatcaaa tccattgact tcattcactt
    18421 ttactgtata attgacatac tcacctgctg taagcattgt ctttaataac tgggcttctc
    18481 ccattactcc ataactattt tggagttcgg catcctttaa atctggaaat actgtagatg
    18541 ctacacataa ttcagctaca taactattgt agtcaatttc acttgtatat tgtccagtat
    18601 gcttaccatt gttaccaatc acttttactc ttttagtaca ctttcttctt agtgcttcgt
    18661 cttcttcaga tgataaaact tttaattccc attcaactgg tttcccttct ttatctaaaa
    18721 atctgttact cgctacatat tttacattat caacctttat tgcattttga cttaaaaaag
    18781 cacttaaatt actcatatta ttctaatctc cttttatttt aatttttcat ataaaaaata
    18841 cacatatata atttataaat gtgtctttta ctccattcct gccaataaat taaatttttc
    18901 tactaattcc caatcctcaa aagtgaaatc catatcttca tctaaatact caccatcagc
    18961 atcaaattta gtaattattc cactgtccat attacaatct ttaagtacta ctgtctgtct
    19021 tcctacagca gatgtagggt cttcatttgt aacttgtatg tcaaaataaa tatcctcacc
    19081 agtttcttta tatctgtaaa gtaattctct aaaaatagaa gtattataat gaaatgttgc
    19141 acttccagta tttgtactcc cagttgtttt atttcccttt gttgttcttc ctagaattgg
    19201 aacttcactt ttattttttt ccattttagc ctctaaatct atagcttgca taaaattata
    19261 tcttttacct tctatagtta taaaacattc agctttcttt gcactaactg tatctttagc
    19321 atttattgtt tgagccatat tatcactctc ctctctaatt aactgaaaca gtcatataaa
    19381 gcttactcat agcatttatt accttaacag catcagatac tatgacagtt ttcttatcat
    19441 ttccaagctc tacactaaca tcatcagttt taaaatcttc tattgccctt atattctcta
    19501 attctttatg gtgtttaaca acatcattcc agaaacttat tcttcctgcc ttatcattcg
    19561 gaactttacc taaatacttt tcattaaata aagttgcaat atcattagca atttggtcaa
    19621 gtactctaac actttggtta cttgaaaaat cgtcattttt atcatctgta aatgatacaa
    19681 aagtatttat atcctctaac acatgaactt catctcccac cttgtggaat ataaatttac
    19741 cagtttttaa agcttcttca agttgtattt gtgtataatt aacatcaaca tcaaactcac
    19801 catcatactt tttattagta ttagatttat ttatatcgca tcctgctata gctccagtag
    19861 cccaataaat caagctagat tcaactaaat ctttatcttt aatcttattt tctatagaca
    19921 ctacaccttc ataatctgca tcacttttct tatatagtac tgtttgaaac ttagctccta
    19981 ccttatctct cattctcttt gtaaattcta caaataaact tttaatttct gttgttgtag
    20041 ccaaacatcc taaagcatta aagctatagc tttctatttt atccaagaaa gcttggtact
    20101 ctgctcctgt cacagcttcg ccattagttc caccagtaaa tacaaatcct gcacttgctt
    20161 ctagtgttgc atccttcttc caagtgatat agtcattgtc ttgtaagtct gtaataacct
    20221 ttgctatttg agtatctacc ttcttattat ctaaaagtgt tacaacatca aatttagtgt
    20281 tatcatctat atttgttgtt actattatct ttaaatcatt acctcttatt ccactatact
    20341 tagctatagc tatagtacaa ctggctttaa cgcctttatt taatttataa aaatatccca
    20401 accttatatt tttgaataaa tctctcaagc ctttcagctt ctcatgagta taatcatatc
    20461 caaaatactt cactgaatac ttctcaaaat catcactggt tacttgaaat acgtcttcat
    20521 ctatgcccca atctaactct aaaggtattg caacaatacc tctatccgat aatgaactgg
    20581 ttgccctctt agctgagata aaatttatat agctacctgg taatatttta ttctgtgtta
    20641 caaatgttcc tccacctaac gccaaattaa ctcactcctt tcataaagct atttattatt
    20701 tcctctacct ctgagaagga atataactca ttttctttta aaattgcatt taataagtct
    20761 tttctattta tatacttctt agaattaact atctgctcct tagtaaactt gtaatcggtt
    20821 cctttgctta atgtcttact caaaattatc acctctcttc aaaccaccga ataactctac
    20881 tgtatccatc ttattgatat cattattttt tatagtaaaa tagttataat caacaaagaa
    20941 atgaagtaca ttatctacaa tttcaaagtt catatttgta cctctgacta aatctccatc
    21001 aatttctata tactctaatt cctccagtag catctcagct acctcattta tttcaaatga
    21061 tttatcatta cttttgggaa aataatgtac atcaaaagag ttctttttta atgtcctgcc
    21121 acttggatat gatactttgc ttggatttaa aggaacaata aaaaagcaag gttcatttat
    21181 accttgctcc acatcttcac tataaattgt ataactctct ccaaatgttt tatctaattt
    21241 aatagatatt ccatcaatta tattattaag catcaaatac tcctttaagt aatattaata
    21301 actttttctc tataatctta tcaatttggc tttgtagttc catctctgaa attgttaaga
    21361 aatgttgtcc tttaacccat cccttaccat ctttagttct atgcccgaaa tttacataac
    21421 ttgcatattc agtcggatta acaacttcta taatataatt atttccttgt ttatacacag
    21481 gaagcgacct agcataagcc actccattcc atccttgtct taagaatcct gtatcaactg
    21541 gtattcttct aattactttc ccaagtaatc gtgctgctaa ttctcttgct gcatccttgc
    21601 aaaacttatc taaatcaatc tttgtaagct tctccatctt tttacaaact cttttaaact
    21661 ctctaaaatc aacactgccc catatagcca ttatgcttta tccttaaata actcaagtat
    21721 tatttcttga tgatttggat atatagctga ttctccactt cttacatact ctttatcatt
    21781 tataataagt tttgaacctg ctttaatttc tatatctgga gatataaaga gtttaatagt
    21841 ttgctctagc ttagctactt tcccttctgt agcagaaact atatttttat atgaaagttt
    21901 acatggttga ttttctaata caatcacttc tttattgtta gttcgttttg ttacagggtc
    21961 tttgattggc tgatactcaa ctatagtaca tttatctcta tataacattt ctattgcttt
    22021 tctagtttta cttaccatct taagcaccta aaggttaata tcttattctt gccataagta
    22081 gtaagataag ctattaagct atcaaagcgt tgttctggtg tttgagaacc acttcctata
    22141 gcaaaatcta cctttgtatc accttctgat atagactttt ctacagcttc aaagttaatg
    22201 ctttctatat ctaattgacc catatttttc ttggtaaata agaactctcc aactatcata
    22261 tcaacttcaa tttctttcaa ttcaattggc atagttttta tattacaatc tagtttaata
    22321 atattttcta ttttttctct tacaaaacct attaaccact tatctccatc ttttaatata
    22381 tatccaaaac tttcaagtct tttttctatt tcatcaatta tattattttc cataattttc
    22441 acctactttt tagtaagttt atttttctct ttaagctgct tattttcttc ttctaaagac
    22501 tcaacttttg accttaaaat attattttca gctattaaat cttttacact taatgacttg
    22561 ccatacttta ctgccttacc agtttcatct atcaaatcat atcccatctc taagaaatca
    22621 tctattttac attcttctat ggttagtatt ctatttagtt tttttacttg tggcactata
    22681 tatcactcct ttttctatgc ttcaacaaca aattgtattg catcagcttt tttatttaat
    22741 ataaatacat cctcaaaact ttcttcaaag tagaagtatt ttccctctgt aactgctgtt
    22801 ggttcgtcta acttagagaa ctgataagaa acaggtgtaa ttattgcact tgggtgaact
    22861 aaggacataa agatttgttt agctcctgct cctactttcc atccagttgt aaaatcatat
    22921 gcagttttca ttagattaga tggtacttta attattttaa ctgtgtcaat atcagttgtt
    22981 tgacgattaa gagaagttcc tgcatccttt atatttactg ttctttgtat ctcttttgca
    23041 tttttgataa gtgtatttac tactggagta acatacaata ttcttccatt ttcaggtact
    23101 ctagcttctg tcattttttc cattaactta tcaaatactt ctaatacgtt tgttgttgta
    23161 agaacagttg tatctgctgt gttacctaat gcggtccaat cagcatatat tttagatata
    23221 cagtaagcat ccatctctgg aaacttttgt tcctcattat atacttttgt tatattgcct
    23281 attgaagcca cataattagt ttggtttata tctgctggat gaaccaatgt tgaccatttc
    23341 ctttgattag ttaatacctt aggttcccaa gcattatcat agtttctttg agctactgct
    23401 attgtatctc tgtttgaatc tactcttcca gttgtagata tagttggtat ttctattgtt
    23461 ttagaaccag tccatctata tcttccatta tttggtgttg catacaaatc cccgaagttt
    23521 aaagtataag gatatgcttg tgctaaaaca tttgaatatt cttttgcata atttagtgct
    23581 gccattttat ttcctcctat ttattattat tttcatgagg tcttacccca gtaaaattaa
    23641 aaccaaaatc atttatctta ggctcttgcc ctggtgttat agtatctatt ttaggttctt
    23701 caccttctag tgttgcatta aacaaataat ctttatcctg tttcaaagga tttatttgct
    23761 cttcaaaagc tttttgtctg tctttactat ttcttagtgc ttctatatct aaatgagctt
    23821 ttaatgctat ttcatctcta catttaacag atttaaaagc atcacctaac cagtaattaa
    23881 aatctttttc ttcaatttct tttttgtagg tttcttccaa agttttctta tcagtttcat
    23941 aagttgtttt tagattctct acatcttctt ttgtcatacc tccttcaaac tttttaatag
    24001 tttcattagc tgtattaagc tgtgtttcaa gatttgtata atcttcttga gtaattgtag
    24061 tctcttttat tttcttttct atagattttt gaagagaagc tacatcaatt ttgttatcct
    24121 ctacttttat tccttctagc aattctttta accaatccat tttaaatttc tcctttcatt
    24181 ttttacaaaa taaaagcatc tacttatttt taagtacatg cttagtcatt ccttatttat
    24241 attttcgata gattctattt cattttcata aacttcaatt ccataaccat ccctagctat
    24301 tgatatactt gctatttctg gttcattatc taaagcttgt gtatatccat cacactttcc
    24361 tcttattatt tgcttatcta cacaagttat ttgaacattt ttccctacat attcccataa
    24421 tttcatttta tttttcctcc ttataaagct ggtactatat gtgttccagt tttggaataa
    24481 tgtatcttaa acttatttgt aagagttttt tcacccgtaa tattattaac attgacccct
    24541 atattcttat caacttctat aagttctttt ttatcccatt ctccacttcg attaaatttt
    24601 atgattccat tgccagcatg cttattcaca agttcttgag cttcttcttt tgttatagtt
    24661 aaatagcttc ttccttctat ataattatta tgctctttta aatgttttcc ttgtttccca
    24721 tcatgaatat ttaaattata tttaccattt ttaatatctt cttttatgct atctattata
    24781 gcactatttt ttatttctaa gatactatta tgtttaacat acttctcata ccactcatta
    24841 tacttcatac tagatggtac ataatatgtt tttccatctt ctcctcttgc tgctctatag
    24901 ccttcttcat cctcgaacca aggagctgtt gttgtcctac aacgacaatg aaatggtgga
    24961 gctgttattc caacttgata atctttcata tcaaatattt ttccatccag ctctctacat
    25021 atatttgaag ttcttaaatc taatgtagca ataatctcat atttctctac atctaaatca
    25081 ctaaaacaat cttttctact tgctgatgca aagaaagctg attcagtcat tatcaaattc
    25141 ttagcttgtg atttagatac attaaatctc ttagcaaagt catttactaa attttttgga
    25201 ttttcacctc taataattga ttgagttagc ttagtatgta gttcatttac taaagtaggt
    25261 ctatacttac cccaaattct ttcactaaag tttaatccat ctgttgccca tggtttagag
    25321 ataattttat ttattctatt agtatcaaga ctcattaaac tccaaccaac gtttactcct
    25381 tgttgaacat taaaagctgt atgatagtat ccacttgtat aaatatctct cattagttta
    25441 tcaataccat caagttcatt tccatataaa acttctactt gttgctgtat ttgtaacttt
    25501 aaagcttcaa gtcttgttat atgaactctt gcactagcat tttctaactc tttcatccac
    25561 ttttgattta tagcattttc tttaccatat ttaatatatt cttctacact ccatttaaac
    25621 tcttctagtt ctcttgtatt tagtagtttc ttagcttcca ataaagatat gccttcactt
    25681 tcagtaaatc tgttgtacca tgataatata tctttttcta tactatttat agctagttta
    25741 tattgctttt ctaattcaag ataatatttt acacttttgt tattttgagc ttcttctaat
    25801 tgttcaaatc tctttctcca ataatcttta tatttcatct ataacaccat cttgattatt
    25861 aggaattaaa tcatcatact ctttttgagt atcttcctgt tttttaagtc tctcaagttc
    25921 gtcattaaca tcctcaaccc aaggatgatt ggaaacaata gtttcatctg atacaattcc
    25981 agttgattta gctgccatat ctatcttttc agcttcattt attatcatag agtgattaaa
    26041 agtaatttga actgttttat aatcatagct cttactacca cttatcttta aatactcaca
    26101 cacaaaccat aaaagctctc taattgcttt tttaaacttc ttttcagtct tagaacattt
    26161 taagtcaagt agtgaatata aaaatttaag tgctacaccc gatttgtcac ctgtgttttg
    26221 agattctgga ttaactcctt gaccaaagat aattatattc ttttctaatc tatcaagaag
    26281 ctccttttta gcttcaactg gtatatttat ctctagttta tcaactccac ctccaccatc
    26341 tactttaatt gatttatagt atcttatatt atctataaac tcttgtagac ttgttcctgg
    26401 atattctttt aatacataaa taacctcttg tatttcatct aaattatctg ctagtgtaga
    26461 aatattattg tcatatatat ctattaatga cttatagaaa gttaaatctg agacacactt
    26521 ttcattattt ttaaaaggta taaatggaac tttaccccat ccctgttctt tgttatttat
    26581 tctaaaatga ccttcttgta tatcagtcat ttttccgtat tcatcatata aaaattcttg
    26641 aataaaacta ttacctcttt caataaaata agttacgtca ttttctgtgt agtactcaac
    26701 tctttttatt ttatttccat ctatatcttc aatataataa aacctaataa atgcaactaa
    26761 ttccctctgt cttttactat cccaaatagg aattgcttct tcggctggta ttattacata
    26821 tttaaactca ccttttctat taatatatgg atgtaaccat tcaacccctt tattactagc
    26881 attgagatat agttctgtta ttgtatcgtc aaactcttct cctagtaagt catttaaaag
    26941 cttagtgaga ttatcatcat ctgcattaaa tactatggga tttccgacac tatagcctac
    27001 cttttggtca actaaaagct tatggtagtt gttaattgct ttattattaa ctttagtaaa
    27061 atcatcaacc ttagctccat ctaagagata atatcttctc ttattgttta catcagtatt
    27121 accataatag tattcttctc cttgtttata tttttctggt ctatgcttta agatgtagtg
    27181 ttctatgact tttactaggt taaaagtgct ctcttttttt aactgaactt ttattaaatc
    27241 tgtttcactt atataaatat ttaacacctc ctttacttta agaagcttat tccattattt
    27301 ttaagcttat tatctataga atatctaagc gcagccattg catcatccat aaactcaact
    27361 ggttcatcaa gatataatcc agttctttca tcttgtttcc atttccattg ttgtatttct
    27421 tttatggtat tagtgcaact aggatgtaca tgtattctta attgtttcaa ataatctatt
    27481 tgagctttaa cacttcctgg tcctttttta actcctttag ctttatatcc tgcactcttc
    27541 cacatcttaa ttctatctgg ttcagcacta tcacattaca taaatagagt cttttctaaa
    27601 cctatactat ttgcaatctt tatgatttct gaggtatcca tttcatgtac atatatttcg
    27661 ttacatatat ataactctcc atccttaaag ccaattctaa gtactacatt tgcatggtta
    27721 aatccaaagt cttgtgataa cctcatattg tcaaaatact caaattctgt aggaaattca
    27781 tgtataacat aatttttaag tattgctcca ccagtttctc cccattctcc aagaccatag
    27841 actttgtacc cttctgggtc ttgctctttt ctcatttgca ttcttctgta gtaagcctca
    27901 tctatgaatc tattttgtag ataagtacta tgatgagtaa atatatcatc atttttatag
    27961 tcaaaatact ttctttttat ccaatgagta gatgagactg gattaaatgt aaatgtcatt
    28021 tgatagtata ggttaggatt agttaaaata cctcttaaac ggtcatctag tatgtctatg
    28081 tcactttcca taagttctgt agcttcttca caccaaaccc atgttaattt tcctttcgag
    28141 aagttaattg attttaattt ttctctttgt tttgcatcat taactcctct gaaaattata
    28201 gagttaccag taactttact cttaatttct aaaggattta aagtagtttt ccaatactta
    28261 tcagcttgtt taccataaat acgatttata gctcctgtaa gctctgcata cgttgaatac
    28321 ttatgtgtag cttctgactt tctaactact aatagattag ctccttgata cttcttatct
    28381 cctaacttta gtatatagtc ttgtgctaca ttaacagatt ttccactccc tgctgaacct
    28441 ttcattgctc tgtatctttt tttagtaaaa ttggcttcct tgaaatctgg attaaaattt
    28501 actctaacta tcatttctat caccataatc tacacttatt ttcaactcat catctccaat
    28561 atcatcttta cttaggttat caacttcaca tttcaacttc tcaactctat ttttctgctc
    28621 ctctgtagcc aaattccaat ccttatgaat catttcatca tactgtttaa ttaaacttct
    28681 aagttcactc atagccctac tctgtgcatt aagaaaagat gcttgcctat cccatgcaaa
    28741 ttgaaattca tactctatct tctcaccatt ttctgtgctt tcatatttct ttaattcttt
    28801 aaccatttct tccttgcctt taacatacat tatcttctgt gctcttatta ttgctgcgta
    28861 ttgaattgtt atctgttccc aaagaatatc aaatttatct tttatagata tttcttgtat
    28921 caattcccta gtttcttcgg gtagatattt tgagaagaaa ccaaactttt cagcgttctt
    28981 attctctttt ggagcaccat gaccaactga atttttatta gaaaagggtg cacctctttt
    29041 atttataggt gcacccttct ttttttcact agcccagttg tatcttttta tccatgactt
    29101 taaagtgttt aagctaatgt catactttga tgatatttcc ttttgtttca ttccttttaa
    29161 gtaatcttgt tttacctttt ctttgacatc ttgcacatca ccacctcttt atttgtttgt
    29221 tttgggaata aaaaaacaat cctatttaat aggattgttt caaatctagt ctcctctatc
    29281 tttccaggat aaatgaaaca tacttattat taatgtagtt agagataaaa ttatatgaac
    29341 tgtaaaaaat attaaaacat agaaaattat attagaataa tatcgagtaa gcattattgt
    29401 attgataaaa catattacaa caaccaaaat ctccattatt acagtataat atgcttttaa
    29461 ctgtatgaat tgaaagtatg atatattaaa tccatttacc tttctttctg tttttatatc
    29521 ttttgcttct gatattgatt tgttatatgt agtaaacaat aatgtaataa tgcaaactcc
    29581 aaaagctact aatagtcctg acaaagttag tacatctttg tttaaagaaa ttacaacttc
    29641 tttattaatt gacgaattaa aaaacatgta tgaaataata catattaata ctggtgttaa
    29701 aactaactct actacttctt tagaatttat agccttgtaa taattgagta agatatttaa
    29761 taccttttta tacattttaa cacctccttc gcaatctatg cttcactcat agcaacttct
    29821 tgatacatta aattaaaaaa aactgaatta ttctcaatta aatcatctat caaattattc
    29881 atcttatcaa aaatgtcttc actatctata tgattatcta tatcaagaga aacctctatt
    29941 tctctacaca actttatacc ctcagtatcc aactttaatt ttctcttgtt tttctctccc
    30001 tcaattatta tcctatttat ttttttattt ctaaattcct ctcctttaat atatttttcg
    30061 caacatcttt taacttcact aggtaaatat ctagttctaa atgtgggctt atatatcaaa
    30121 tcgttatgtc gtcttgaatt atttaaatta gaaaacaaat tatcttcatc agttccaaac
    30181 ttctctctat caacatttaa agttattttt gaaacaccat ctaaatctaa tatttgttct
    30241 ataaaatctt tacttgctat ttgtgtaata ttaattgatg tttttgacat attattatca
    30301 aatttattat tttcaatcat atcctcataa tatagatgta agtttttact tagttcaatc
    30361 atgggaaccc ccacaaaaac tctttcaaat aatactactg ctatattatt ttccttcata
    30421 aatttaataa ataggtgctg cttattgcta tctccttgat ccattttttt attaccctct
    30481 acttctttag tatgagcatt tataatattt gtattctgat tgtatttagt atattcaagt
    30541 ttcaccttga ttattaaatc atcctcgcta cattttactt ctgtaatcca aaacttttta
    30601 tcttta
//
LOCUS       DAEMHW010000030        30056 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEMHW010000030 DAEMHW010000000
VERSION     DAEMHW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30056
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.030"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            192..1673
                     /locus_tag="KPK67_001135"
     CDS             192..1673
                     /locus_tag="KPK67_001135"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBG2458130.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            1894..2049
                     /locus_tag="KPK67_001136"
     CDS             1894..2049
                     /locus_tag="KPK67_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458131.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            2194..3591
                     /locus_tag="KPK67_001137"
     CDS             2194..3591
                     /locus_tag="KPK67_001137"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBG2458132.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKNDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            3576..3989
                     /locus_tag="KPK67_001138"
     CDS             3576..3989
                     /locus_tag="KPK67_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBG2458133.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            4095..4853
                     /locus_tag="KPK67_001139"
     CDS             4095..4853
                     /locus_tag="KPK67_001139"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBG2458134.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLKEVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            4866..5603
                     /gene="rlmB"
                     /locus_tag="KPK67_001140"
     CDS             4866..5603
                     /gene="rlmB"
                     /locus_tag="KPK67_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBG2458135.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            5606..6133
                     /locus_tag="KPK67_001141"
     CDS             5606..6133
                     /locus_tag="KPK67_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBG2458136.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            6198..6845
                     /gene="sigH"
                     /locus_tag="KPK67_001142"
     CDS             6198..6845
                     /gene="sigH"
                     /locus_tag="KPK67_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBG2458137.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            6919..8112
                     /gene="tuf"
                     /locus_tag="KPK67_001143"
     CDS             6919..8112
                     /gene="tuf"
                     /locus_tag="KPK67_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG2458138.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVE
                     DVFSITGRGTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLDQAQAGD
                     NIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFDGYRPQF
                     YFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRTVASGVV
                     ATIIE"
     gene            8484..8795
                     /gene="rpsJ"
                     /locus_tag="KPK67_001144"
     CDS             8484..8795
                     /gene="rpsJ"
                     /locus_tag="KPK67_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBG2458139.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            8887..9516
                     /gene="rplC"
                     /locus_tag="KPK67_001145"
     CDS             8887..9516
                     /gene="rplC"
                     /locus_tag="KPK67_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBG2458140.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            9546..10169
                     /gene="rplD"
                     /locus_tag="KPK67_001146"
     CDS             9546..10169
                     /gene="rplD"
                     /locus_tag="KPK67_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBG2458141.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            10169..10459
                     /gene="rplW"
                     /locus_tag="KPK67_001147"
     CDS             10169..10459
                     /gene="rplW"
                     /locus_tag="KPK67_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBG2458142.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            10489..11319
                     /gene="rplB"
                     /locus_tag="KPK67_001148"
     CDS             10489..11319
                     /gene="rplB"
                     /locus_tag="KPK67_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBG2458143.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            11354..11635
                     /gene="rpsS"
                     /locus_tag="KPK67_001149"
     CDS             11354..11635
                     /gene="rpsS"
                     /locus_tag="KPK67_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBG2458144.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            11667..12002
                     /gene="rplV"
                     /locus_tag="KPK67_001150"
     CDS             11667..12002
                     /gene="rplV"
                     /locus_tag="KPK67_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBG2458145.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            12025..12840
                     /gene="rpsC"
                     /locus_tag="KPK67_001151"
     CDS             12025..12840
                     /gene="rpsC"
                     /locus_tag="KPK67_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBG2458146.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            12876..13307
                     /gene="rplP"
                     /locus_tag="KPK67_001152"
     CDS             12876..13307
                     /gene="rplP"
                     /locus_tag="KPK67_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBG2458147.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            13309..13512
                     /gene="rpmC"
                     /locus_tag="KPK67_001153"
     CDS             13309..13512
                     /gene="rpmC"
                     /locus_tag="KPK67_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBG2458148.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            13536..13790
                     /gene="rpsQ"
                     /locus_tag="KPK67_001154"
     CDS             13536..13790
                     /gene="rpsQ"
                     /locus_tag="KPK67_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBG2458149.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            13816..14184
                     /gene="rplN"
                     /locus_tag="KPK67_001155"
     CDS             13816..14184
                     /gene="rplN"
                     /locus_tag="KPK67_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBG2458150.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            14208..14513
                     /gene="rplX"
                     /locus_tag="KPK67_001156"
     CDS             14208..14513
                     /gene="rplX"
                     /locus_tag="KPK67_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBG2458151.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            14543..15085
                     /gene="rplE"
                     /locus_tag="KPK67_001157"
     CDS             14543..15085
                     /gene="rplE"
                     /locus_tag="KPK67_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBG2458152.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGIKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            15103..15288
                     /locus_tag="KPK67_001158"
     CDS             15103..15288
                     /locus_tag="KPK67_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBG2458153.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            15321..15719
                     /gene="rpsH"
                     /locus_tag="KPK67_001159"
     CDS             15321..15719
                     /gene="rpsH"
                     /locus_tag="KPK67_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBG2458154.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            15750..16292
                     /gene="rplF"
                     /locus_tag="KPK67_001160"
     CDS             15750..16292
                     /gene="rplF"
                     /locus_tag="KPK67_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBG2458155.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            16328..16696
                     /gene="rplR"
                     /locus_tag="KPK67_001161"
     CDS             16328..16696
                     /gene="rplR"
                     /locus_tag="KPK67_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBG2458156.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            16717..17226
                     /gene="rpsE"
                     /locus_tag="KPK67_001162"
     CDS             16717..17226
                     /gene="rpsE"
                     /locus_tag="KPK67_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBG2458157.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            17241..17426
                     /gene="rpmD"
                     /locus_tag="KPK67_001163"
     CDS             17241..17426
                     /gene="rpmD"
                     /locus_tag="KPK67_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBG2458158.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            17459..17902
                     /gene="rplO"
                     /locus_tag="KPK67_001164"
     CDS             17459..17902
                     /gene="rplO"
                     /locus_tag="KPK67_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBG2458159.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            17946..19214
                     /gene="secY"
                     /locus_tag="KPK67_001165"
     CDS             17946..19214
                     /gene="secY"
                     /locus_tag="KPK67_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBG2458160.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            19231..19881
                     /locus_tag="KPK67_001166"
     CDS             19231..19881
                     /locus_tag="KPK67_001166"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBG2458161.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            19882..20628
                     /gene="map"
                     /locus_tag="KPK67_001167"
     CDS             19882..20628
                     /gene="map"
                     /locus_tag="KPK67_001167"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBG2458162.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSNISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            20650..20919
                     /locus_tag="KPK67_001168"
     CDS             20650..20919
                     /locus_tag="KPK67_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBG2458163.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            20957..21175
                     /gene="infA"
                     /locus_tag="KPK67_001169"
     CDS             20957..21175
                     /gene="infA"
                     /locus_tag="KPK67_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBG2458164.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            21211..21324
                     /gene="rpmJ"
                     /locus_tag="KPK67_001170"
     CDS             21211..21324
                     /gene="rpmJ"
                     /locus_tag="KPK67_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBG2458165.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            21477..21848
                     /gene="rpsM"
                     /locus_tag="KPK67_001171"
     CDS             21477..21848
                     /gene="rpsM"
                     /locus_tag="KPK67_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBG2458166.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            21884..22282
                     /gene="rpsK"
                     /locus_tag="KPK67_001172"
     CDS             21884..22282
                     /gene="rpsK"
                     /locus_tag="KPK67_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBG2458167.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            22314..22937
                     /gene="rpsD"
                     /locus_tag="KPK67_001173"
     CDS             22314..22937
                     /gene="rpsD"
                     /locus_tag="KPK67_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBG2458168.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            23015..23962
                     /locus_tag="KPK67_001174"
     CDS             23015..23962
                     /locus_tag="KPK67_001174"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBG2458169.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            23983..24324
                     /gene="rplQ"
                     /locus_tag="KPK67_001175"
     CDS             23983..24324
                     /gene="rplQ"
                     /locus_tag="KPK67_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBG2458170.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            24444..25277
                     /locus_tag="KPK67_001176"
     CDS             24444..25277
                     /locus_tag="KPK67_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG2458171.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            25265..26131
                     /locus_tag="KPK67_001177"
     CDS             25265..26131
                     /locus_tag="KPK67_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG2458172.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEVFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            26125..26928
                     /locus_tag="KPK67_001178"
     CDS             26125..26928
                     /locus_tag="KPK67_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBG2458173.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            26960..27691
                     /gene="truA"
                     /locus_tag="KPK67_001179"
     CDS             26960..27691
                     /gene="truA"
                     /locus_tag="KPK67_001179"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG2458174.1"
                     /translation="MRNIKIKIQYSGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            27811..28242
                     /gene="rplM"
                     /locus_tag="KPK67_001180"
     CDS             27811..28242
                     /gene="rplM"
                     /locus_tag="KPK67_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBG2458175.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            28271..28663
                     /gene="rpsI"
                     /locus_tag="KPK67_001181"
     CDS             28271..28663
                     /gene="rpsI"
                     /locus_tag="KPK67_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBG2458176.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            29122..29874
                     /gene="cwlD"
                     /locus_tag="KPK67_001182"
     CDS             29122..29874
                     /gene="cwlD"
                     /locus_tag="KPK67_001182"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBG2458177.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT        12227 a         3403 c         6100 g         8326 t
ORIGIN      
        1 aatttttaga aaataaatga cttaaaagaa agatttatga aattaaaact aggattttct
       61 gatataataa acatgtgtat ttttaaataa aaaggatatt attattagat ttgattaaaa
      121 ataaacttgc aaaacaatgt actagtttaa tattatagtt atattgaata aaatatcagg
      181 aggaagtaaa aatgaatgta agagtaaggt ttgcaccaag tccaacagga tttgtacata
      241 taggtagttt gagaactgct ttatataact atttatttgc aaaaaaaatg ggcggagaat
      301 atatattaag agtggaagat acagaccaaa gtagattagt agaaggtgct atagagaata
      361 tgcttaatgc tatgaaatgg gctggggtca accatagtga aggtgttata ttagatgata
      421 gtggaaaagt agtacaaaaa ggagaatatg gtccatatat tcaatctcaa agattagata
      481 tataccaaga atatataaag caacttttag atagtggaaa agcttattac tgtttctgta
      541 caaaagaaag actggatgaa gtaagagatg ctcaaagagc agcaggagaa acagctaaat
      601 atgatggtca ttgtaagaat ctttctaaag aagaagtaga agcgaatata aaagcaggaa
      661 taccatatgt tataagatta agattgccag agaatcatac aattaagttt acagacttag
      721 ttagaggaga tatggaattt aatactaatg acttagatga ccaagtatta atgaaaacag
      781 atggatttcc aacttatcat tttgcagtag ttgttgatga ctatttgatg aaaattacac
      841 atgttataag aggagaagaa tgggtatcat ctactccaaa acatgtttat ttatatgaag
      901 cttttggatg ggaagcacct gtatttgtgc atttaccaaa tatacttaat aaagaaaaga
      961 aaaaattaag taaaagacaa ggtgatgtag ctgttgaaga ctttaagaaa aaaggatatt
     1021 taccagaggg attagtaaac tatgtagcat tagttggttg gtctcctgaa gataatcaag
     1081 aattgtttac aatggaggaa ttagaaaaag ccttctctgt agaaagagta tctaaaagtg
     1141 gtggagtatt tgatactgaa aaattaaatt gggttaatca gcactatata aaagatggtg
     1201 atgatgctta tctaacagac ttagcaatac catttttaat agaagatgga tttataacag
     1261 aagaagaagc tactaataaa tatgatttct taaagagtat gatatcagta cttaaagaga
     1321 aattacaata tgtaaaagaa gttactgaac atgcaagtat attttttgga gataaaatag
     1381 aggtagaaac tgaagaggga agagagttct taagattaga acatatacct actttaatag
     1441 atgctttgag agaaaaaata gaaaaaacag aggtattaaa tgctgagttt gttcaagcta
     1501 tgcttaaaga aatacaaaaa gagtacaaaa taaaagggaa aaatttattt atgggctcaa
     1561 gaataatttt gacagggcaa atgcatggtc cagatttacc aaaagtaatg gaagttttag
     1621 gaaaagaaac ttgcttaaat agaatagctt atgtaaaaaa taatatatta tagtcataaa
     1681 agtccgctaa tttagaagcg gacttttttt gtgttagtat ttggtgaaaa tatctgctta
     1741 agaaaatgaa ggtatttaag gtaataaatt taatatataa aaagcaaagc aaaaatataa
     1801 ataaaatatg aataataaac taataaaata ttaagcatag aagaagggaa tctattattt
     1861 tagtttgatt taattattaa gggggtaatt actatgtata gtaatattga tgatgttaaa
     1921 aaggaattaa aggagctatg cttagaatat gttacaattt tagaaaaatt aaaagatgaa
     1981 aaaatgatta cagaagaaac ttttgaaaag tgctcttctc aaaaaaaaat atttttagaa
     2041 gaacaataga ggatggtaat agtattatta ttatgtagtt tgaaagatag tacgaaaaat
     2101 gatagtaata ctattattta ttgcaatttt caactataat ataatatata atgttaagaa
     2161 gataatttat aaaaatacag tgaggtgatg agtgtgaaag tttataatac attgactaga
     2221 acaaaagaag aatttgttcc attagaagaa ggaaaggtaa aaatgtatgt gtgtggtcca
     2281 acagtttata actatataca cataggtaat gcaagaccat ttataatatt tgatactcta
     2341 agaaggtatt tagagtatag aggatatgat gtaacttatg tacaaaactt tacagatgtt
     2401 gatgataaga ttataaatag aagtcatgag gagggaatat ctcctgaaga agttgcggct
     2461 aagtatataa aagaatattt tgtagactgt gatgggctag gaataaaaag agctactgtt
     2521 catcctcagg ttacagataa tatacaacag attatagagt ttataaaaga gttggaagat
     2581 aagggatatg catatgcagt aaatggtgat gtatattttg atacaaataa gtttgaaggt
     2641 tatggaaaac tttcaggtca aaaacaagaa gatttagagg caggggctag aattgaggtt
     2701 aatgaccaaa aaagacatcc tatggacttt gttctttgga aggcaaaaaa agaaggtgag
     2761 ccaggatggg atagcccttg gggagaaggt agaccaggat ggcatataga atgttctgtt
     2821 atgtctaaga gatatttagg tgaaacaatt gatatacatg caggtgggca agatttaaca
     2881 ttcccacatc atgaaaatga aatagctcaa agtgaagcta gaagtggaaa aacattctca
     2941 aaatactgga tgcataatgg atatataaat ataaatgatg aaaagatgag taaatcaaaa
     3001 ggtaatttct ttacagtaag agatatatca aaattatatg atttagagat agtaagattt
     3061 ttcatgttat cagcacatta tagaaatcca gtaaacttta gtgatgagat gttaaatcaa
     3121 gctaaagcag gtcttgaaag actatataat actaaagaaa aattagaatt tacgctaagt
     3181 aatttagtag aatctccttt aacagaaaaa gaagtagagc ttgttaaaga attagatgac
     3241 tttagacaaa aattcataga tgctatggat gatgatgtta atacagcaga cgcagtaagt
     3301 gtaatatttg aattagctaa gttaataaat tctaatgtag atgaaaattc ttctttggag
     3361 tttgctaaaa aatgcttaga tgaatttaat gaacttacag gagtattaaa tattgtaaat
     3421 aagaaaaatg atactgtatt agataaagac attgaagaat tgatacaaaa aagaactgat
     3481 gcaaagaaaa ataaagagtt tcaattagct gatgatatta gacaacaatt actagatatg
     3541 ggaattgtat tagaagacac aagacaaggt gttaagtgga aaaggatata aaaatggaaa
     3601 aaactgaatt agttactatg tcaccattag ttttagcata cttaggtgat acagtgtatg
     3661 agacatatat aagagaatat ttaattagac agaatacaca aagaaaagtt aatgatttgc
     3721 ataaactagc tataaagtat gtaaaagcta aagctcaggc aactataatt catgaaattg
     3781 aaattgagtt aactgaagaa gagagtaaaa tttataaaag gggaagaaat caaaagtcaa
     3841 atacttctcc taagaatgca gatattatag actacaaaca tgcaactggg tttgaggcat
     3901 tagtgggata tttgtattta aataatgaaa tagagagact tcaatatatt atcaataaag
     3961 gaatcaaaat tattgaaaga gatatgtaaa tattaaagag tgttgtctta ataataagta
     4021 ataaggcaac acttttttta taactaatgt ataataaata tatagaaatt agtaatgaga
     4081 aaggataaaa agttatgaaa gtaaaattaa tatcacatac acctgaacca gaaaaagtaa
     4141 tagctatggc agctaagcta tgttattcac cagttgggac agacgaaata gaaaaagact
     4201 taacagatga aagtatagag aaatttttaa atatgttatt aagtataggt catgggtcaa
     4261 tattagaaca tgcttcattt acattttcaa tagaaggtat ttcaagggct tgttcacatc
     4321 aaattgttag acatcgtata gctagttttt cacaacaaag tcaaagatat gttaaattag
     4381 aacaatttga gtacataatt cctccagaaa tagaaaaaat agaaaaagca aaagagttat
     4441 ttatagactc catgaaaaaa gaccaagaga attatgataa gttagttgaa atattgtttg
     4501 aaaatcatta taatgaccta ataaaaaacg gtaaaaatga gaaaacagct aaaagacaag
     4561 ctgaaaagaa agcaatagaa gatgctagat acgtatttcc aaatgcatgt gaaacaaaaa
     4621 tggtatttac tataaatgct agaagtttgt ttaatttctt tgaacataga tgctgtgaaa
     4681 gagcacagtg ggaaataaga aatttggctg tggagatgtt aaaagaggtt aaaaaggttg
     4741 ctcctatatt atttaaaaaa actggtccaa gttgtgtaaa tggaagttgt ccagaaggaa
     4801 ctatgacttg tggagatata gttcaagtta gagaaaaatt taaggccttg taggaggtga
     4861 ataatttggc gagtatagaa ggaagaaatc cggtaataga agcaataaaa agcgacagag
     4921 aaatagataa aatattaatt gcaaattcag ctaaagaagg ttcaattaag aaaataatag
     4981 gaatggctaa agataagaat ataattattc agtatgttga taaacataag ctagatgaag
     5041 taagtacaag tcattcacat caaggtgtaa tagcttatgc aagtgagtat aagtattatg
     5101 aattagatga gctaatagat ttagcaaaga ataaagatga agacccattt ttcattatat
     5161 tagatgagat aacagaccca cataatttgg gttcaataat aaggacagca gatgcagtag
     5221 gagcacatgg agttatcatt ccaaaaagaa ggtctgtaca cataactcct gtagttgcca
     5281 aagcatctgc tggagcagta gaatatatgc cagtttgcaa ggtaaccaat atagttaata
     5341 caataaaaag actcaaagaa gaagggttgt ggattgctgc agctgatatg gatggagaaa
     5401 ctttttataa acaaaatctt actggtcctc ttggtgtagt aattggaagt gaaggttttg
     5461 gaatatctag attggttaaa caaaactgtg actttatagt aaaaatgcca atgataggta
     5521 atgttacatc tttaaatgca tctgtagcag gtggaatact tttatatgag atattcagac
     5581 aaagactgga taaaagtaaa taggcatgaa aaaaaatata aatcattact taataataga
     5641 tggctacaat attataaatg cgtgggacaa tttgaaagaa ctggctaaag aggacttaga
     5701 agattctcga gaaaagctta ttgatgacat tatagaattt tctgagttta tgggttataa
     5761 gactataata gtttttgatg cttataatgt taaaaattct cgagaaaaag ttgaaaaaag
     5821 aaaacacata acaatagttt atacaagaga acaccaaaca gcagatagtt atatagagaa
     5881 atttataact tctttatcaa agtatgatga tgtaaaagta gccactaatg actatgcaga
     5941 acagcaaatg attttaggaa aaggtgctac tagaatgtct gcaagagaat taaagttaga
     6001 gttagatcgt tccaagaata aaatgaagga aaaaaatatc ggtttgagga aaaaaattca
     6061 gcgaaattgg ttggaggaaa ggttagataa agaaacattg tcgaaacttg agaacattcg
     6121 tagaaagcgt tgaaattact aggttcttta gactataata tacctataaa tttgttttat
     6181 atatggggga gatttacatg ttggtagcaa aagaaaaaag ttatgagtta gtggacaatt
     6241 gtcagcagga tgagtataac attgtattaa gagcaagtga aggggataaa atagcactag
     6301 agtacattat tactaaatac agaaattttg taaaggcaaa agcaaagtca tattttttaa
     6361 ttggagcaga taaagaagat ataatacaag aaggaatgat aggcctttat aaggcagtta
     6421 gagattttga tggaagtaag accaattcat ttaaatgttt tgcggagata tgtataacaa
     6481 gacaaataat aactgctata aaaactgcaa caagacaaaa acatatacct ttaaattcat
     6541 atgtttcttt gaataaacca atatatgatg aagagtcaga cagaacactt ttagatatta
     6601 tagctacaag tatagtaaca gatccagaag agcttataat tagtaaagaa gagttaaaaa
     6661 atatagaatc aaaaatgaac gaattgttaa gtgacttgga attagaagta ctagaattat
     6721 atttaaatgg aaagtcatac cagtttatag cggacaagct taaaagagat gtaaagtcaa
     6781 tagataacgc tttacaaagg gttaagagaa aattagaaaa acatcttgaa aacagaaatg
     6841 attaatagta aaatacttac cataagctta ttgtgagcaa aattaattta tatcgaaaga
     6901 taagggagga aattcaaaat ggctaaagct aaatacgaaa gaacaaaacc tcatgttaat
     6961 atagggacaa taggacacgt agaccacggt aaaactacat taacagcagc aataacaaaa
     7021 acattatatg acagatatca attaggagaa gcagtagatt tcgcaaacat agataaagct
     7081 ccagaagaaa gagaaagagg aatcacaata tcaacagcac acgttgagta tgaaacacca
     7141 aatagacact atgcacacgt tgactgccca ggacatgctg actacgttaa gaacatgata
     7201 acaggagcag cacaaatgga cggagcaata ttagtttgtt cagcaacaga tggaccaatg
     7261 ccacaaacaa gagagcatat actattatca agacaagttg gagtaccata tatagtagta
     7321 ttcttaaaca aatgtgacat ggtagatgat gaagagttat tagagttagt agagatggaa
     7381 gtaagagatt tattaacaga atatgatttc ccaggagatg acactccaat agtaagaggt
     7441 tcagcattaa tggcattaga agatccaaag agtgagtggg gagataagat agtagaatta
     7501 tttgagcaaa tagatgagta tataccagct ccagagagag atacagataa accattctta
     7561 atgccagtag aggacgtatt ctcaatcaca ggaagaggaa cagttgcaac aggaagagtg
     7621 gaaagaggag tactaaaagt acaagacgaa gtagaattag taggattaac agaagcacca
     7681 agaaaagtag tagtaacagg agtagagatg ttcagaaaat tattagacca agcacaagca
     7741 ggggataata taggagcatt attaagagga gtacaaagaa acgagataga aagaggacaa
     7801 gtactagcaa agactggatc agtaaaggca cacacaaagt ttacagcaga agtatatgta
     7861 cttaaaaaag aagagggtgg aagacataca ccattctttg atggatatag accacaattc
     7921 tatttcagaa caacagacgt aacaggagct tgtaagttac cagaaggaat agagatggta
     7981 atgcctggag ataacgtaac aatggaagta gacttaataa actcaatagt tgtagaagag
     8041 ggattaagat tctcaataag agaaggtgga agaacagtag cttcaggagt tgttgctaca
     8101 ataatagagt aatcctttac taaactaatg gatttaatat aaaaaaaaga gggggtaact
     8161 cctctctttt ttgttttttg ttgacaagta gcgctaaagc atatatacta aactagtgcc
     8221 ttgagacaga ttaacaatca agtttaaggt tatgatgttg taatagaaaa taaaattgaa
     8281 aaaaacagaa aaaaaacttg aaaaaatgta acagatgata taaaatataa tagtgtgctt
     8341 gagagattaa gaaaataata attaaatcct agtgaaatac gaactgatat cgatttaaaa
     8401 gttacaacac acccggagca gtgtatcggt attggttgta cgcaacttga aaattaaaca
     8461 tgtgataaag gagggaaaac aaaatggcta agaatgaaaa aataagaata agattaaaat
     8521 catatgatca caaattatta gatttttcag caggtaaaat agttgaaact gctaaaaagg
     8581 ctggatcaca agtttcagga cctgtgcctc taccaacaga aaaacaagtt gtaactatat
     8641 taagagctgt acataagtac aaagactcta gagaacaatt tgaaataaga actcataaaa
     8701 gactaatcga catagctaat ccaacaccta agacagttga ctcattaatg agattagact
     8761 taccagcagg tgtagatata gaaataaaat tataagaacc cctaagatat tatcctatag
     8821 atgattatct tagaatgatt actctaaaag atgtaatccg ctgtaagaat tataggaggt
     8881 gctaatatga aaggaatatt aggcaaaaaa gttggtatga ctcaaatatt cactgataaa
     8941 ggtgtagtta tacctgtaac agctgttgaa gcagggccta tggtcgttac tcaaataaaa
     9001 acagttgata aagatggata caatgctata caaataggtt ttgaagatgc taaagaaaaa
     9061 gctttaaaca aacctaaaaa aggacattta gctgctgcta atgttttaaa gaaacattta
     9121 aaagaattta gagtagattc tgtagaagga tacacagtag gacaagaaat aaaagctgat
     9181 gtatttgaag caggtgcaaa aatagatgtt actggaataa gtaaaggtaa gggattccaa
     9241 ggtccaataa aaagacatgg acaaagtaga ggtcctgaaa ctcacggttc tagataccac
     9301 agaagaccag gttcaatggg agcttgttct tacccaggta gagtatttaa aaacaaaaaa
     9361 ttagctggac atatgggaag cgtaaaggta acagttcaaa acttagaggt tgttaaagta
     9421 gatgctgaca agaaccttat attagttaaa ggagctatac caggagctaa aggttcagta
     9481 gtaactataa aagaagctat aaaggtttct aaataatgac taacctagag aaaggaggaa
     9541 taacaatgcc aaaattaaat gtactaaatg ttagtggaca aaacgttgga gaaatagaat
     9601 tatcagattc tatatttggc gtagaagtta atggacatgt tttatacgaa gttgtaaaaa
     9661 atcaattagc aaataagaga caaggaactc aatctgctaa aactagagca gaagttagag
     9721 gtggcggaag aaaaccttgg aaacaaaaag gaacaggtag agcaagacaa ggttctacaa
     9781 gatctgtaca atgggtaggt ggaggagttg cttttgcacc taaaccaaga agctacaaat
     9841 atacattacc taagaaggta agaagattag ctatgaagag tgctttatct tcaaaagttc
     9901 aaaatagtga agttatagta ttagatgcat taaacatgga tgctcctaag actaaagaat
     9961 ttgctcaaat attaaacaat ataaatgctg ctaaaaaagc tttagtagtt atagctgaca
    10021 aaaacgataa cgtaataaaa tcagctagaa atatagaagg tgttcaaact gcattagtta
    10081 atactatgaa tgtatatgat atattaaaat acgattcatt tataataact acagatgctg
    10141 ttaaaaaagt ggaggaggtg tacgcataat gactaatcca catgatgtaa taataagacc
    10201 agttgtaact gagcacagta tggctgaaat gggtgaaaaa aaatacactt ttgttgtggc
    10261 taaagatgcg aacaaaactg aaataaaaaa agcagtagaa aaagtattcg gagttagtgt
    10321 tgataaagta aatactttaa attacgatgg aaaagttaaa agaatgggta gaactcaagg
    10381 aagaacatca agctttaaga aagcagtagt taaattaact gctgatagta aagaaataga
    10441 attcttccaa ggaatgtagt attataacaa acgaaggagg gaaaacagat ggctataaaa
    10501 aagtttagac caacttctcc tgccttaaga caaatgacag ttttagtttc tgatgaaata
    10561 acatgtaatc aaccagaaaa atctctttta gttaacttaa agaaaaatgc tggtagaaac
    10621 gtacatggta gaataactgt tcgtcataga ggtggaggac aaaaaagaaa atatagaata
    10681 atagatttta aaagagataa agatggtata cctgctaagg ttgctactat agaatatgat
    10741 ccaaacagaa ctgcaaatat cgcattatta aactatgctg atggagaaaa aagatatata
    10801 ttagctccag ttggaattaa cgttggagat actatattat caggattagg tgctgatata
    10861 aaaccaggaa actgtttagc attaaaagat atgccagttg gtactatcat tcataatata
    10921 gaattaaagc caggaaaagg agctcaatta gttagatctg ctggagtttc tgcacagtta
    10981 atggctaaag aaggaaaaaa tgctttatta agattaccat caggtgagat gagattagta
    11041 agcataaact gtaaagctac tataggtcaa gttggaaata tagagcatgg taacgtagtt
    11101 atcggtaaag caggtagaaa aagacatatg ggaataagac ctactgtaag aggttctgta
    11161 atgaacccta atgaccatcc acacggtgga ggggaaggta gatctccaat aggtagacca
    11221 tctcctgtta ctccatgggg taaaccagct cttggataca aaactagaaa gaaaaataaa
    11281 gcttcaaata aactaatagt atcaagaaga actaagtagt aaatttattt ttgctcggaa
    11341 aggaggaata taaatgtcaa gatcaactaa aaaagggcct tttgtccatg caagactttt
    11401 aaagaagata gaagctatga atgctagtgg aaataaagaa gttataaaaa cttggtcaag
    11461 atcttcaact gtatttccac aaatggtaga aaacactata gctgttcatg atggaagaaa
    11521 acatgttcca gtatatataa cagaagatat ggttggacat aaattaggtg agttcgttcc
    11581 tactagaact ttcaaaggac ataaggatga cgaaaaatct aataagagaa aataattaaa
    11641 ttatgactaa gaaaggagga ataacaatgg aagcaaaagc tactgctaaa tacgtacgtg
    11701 tatcaccaag aaaagcaggc caaatatgtg accttgttag aggaaagaat gttgatgaag
    11761 cattagcaat attaaagttt actccaagag gagcagcttc aataatagct aaggttgtaa
    11821 aatcagctaa agctaacgca gaaaacaatc acgaaatgga tactgaaaaa ttatatatag
    11881 catcaatagt tgctaatcaa ggaccaacaa tgaagagatt catgcctaga gctatgggtc
    11941 gtgcaactac aataagaaaa agaacttctc atatagaggt tgttgttaaa gaaaaaaaat
    12001 aattaataga taggagggaa agtaatgggt caaaaggtta atccacacgg cttaagggtc
    12061 ggtgttataa aagattggga ctcaagatgg ttcgctactg ataaaaaaga gttcggaaac
    12121 ttattattag aagaccataa tatacgtaaa ttcttaaaga aaagattata ctcagctgga
    12181 gttgctaaga tagaaataga aagatcagct aacaaaataa aaatggactt acacgttgct
    12241 aagcctggtg tagttatagg aagagctggt gcaggaatcg aagcattaaa agctgaatta
    12301 gaaaaaatga caaagaaaac tataatagtt aatatagtag aagtaagaag tacagataaa
    12361 aatgctcaat tagtagctga gaatatagct ttagctatag agagaagggt tgctttcaga
    12421 agagcaatga aacaagctat acaaagagca atgaaatctg gtgctaaagg gataaaagtt
    12481 tctgcttcag gaagattagg tggagctgaa atggctagaa ctgaaggtta cagtgaagga
    12541 aatgtgccac tacaaacttt aagagcagat atagattatg gtttcgctga agctgataca
    12601 acttatggaa aaatcggtat aaaagtttgg atatgtaatg gtgaagtttt accaactaga
    12661 gacggtgtaa atccaagaga agaaagcaga aagagcgata gaagagacaa caagagagat
    12721 aatagaagaa acgatagaag aggaaatgac agaagaggaa atgataacag aggaaactac
    12781 agaggacaaa gacctcaagg tggatcaaga cctcaaagaa ctgaaaacaa aggaaattaa
    12841 taatagttag gttcaaggaa ggaggaaaaa tactcatgtt aatgccaaaa agagtaaagc
    12901 gtcgtagagt tcatagagga agcatggctg ggcaagctca taagggtaat aaagttactt
    12961 atggtgagtt tggattagta gcattagaag cttcttggat aacttctaat caaatagaag
    13021 ctgccagaat cgcgatgact agatatataa aaagaggggg aaaggtttgg ataaaaatat
    13081 tccctcataa accagtaaca agaaaaccag cagagactcg tatgggtgca gggaaaggtt
    13141 ctccagaata ttgggtagct gtagttaagc caggaagagt tatgtttgaa ttagcaggtg
    13201 tttctgaaga taaagcaaga gaagctatga gacttgctgc acataaatta ccaatcaaat
    13261 gtaaatttgt taaaaaagaa gatttagaag taaagggtgg tgaataggat gaaagctaaa
    13321 gaattaagag atttaacaag cgaagagcta atgaacaaat taaacgactt caaaagtgaa
    13381 ttatttagct taagattcca attagctact ggtcaattag aaaatacagc tagaataaag
    13441 tttgttaaga aggatatagc aaaagttaaa actgttcttg ctgaaagaaa gttatacgaa
    13501 actagagctt aatttggaaa ggaggctttt taaacatgga aagaggaaga agaaaagtta
    13561 gaataggccg tgttgttagt gataagatgg ataaaacaat agtagtagct gttgaagaat
    13621 tcgtacgtca tccactatat aataaacgtg ttaagagaac taagaaattc aaggctcatg
    13681 atgaaaagaa tatatgtaac atcggagata gagttaaaat aatggaaact agaccattat
    13741 ctaaagataa gagattcaga ctagttgaag ttgttgagaa agttaagtag tttttgaaga
    13801 aggagggatt acgatatgat acaacaagaa tcacgtctaa gagttgctga taactcagga
    13861 gctaaagaac ttttatgtat acgtgttcta ggcggaagta aaagaagata tggtaacata
    13921 ggcgacgtta tagttgctac tgttaaaagt gcaacacctg gtggagttgt aaaaaaaggt
    13981 aaggttgtta aagctgttat agtaagaagc aaacaaggcg taagacgtaa tgacggaagc
    14041 tatatatctt ttgatgaaaa tgctgctgtt ataataaagg acgataaaac tccagtagga
    14101 actcgtatat tcgggcctgt tgctagagag ttaagagaca atgaattcat gaaaatagtt
    14161 tctcttgctc cagaagtact ataattaagg aggtgcaata ggacatgatg cgtgttaaaa
    14221 aaggtgacac tgttgtagtt atagcaggta aagataaagg taaaaaaggt tcagttttaa
    14281 aggtatatcc taaaactagc aaagtgttag ttgaaggtgt aaatgtaata actaaacatc
    14341 aaaaaccaag tgctatgaac caacaaggtg gaataataaa taaagaagcc ccaatacaca
    14401 tatctaatgt aatgccattt gatcctgaaa caggaaaagg cgttagagta agatatgaag
    14461 taaaagatgg gaacaaagta agagtatcag caaagagcgg aaaagaatta taataaatat
    14521 tgcttgaaag gagggacctt aaatggcttc tagattacaa gaaaaataca tgaaagaagt
    14581 tgctcctgct ttaatggaga aatttggata caaaaacgta atggagatac ctaagttaaa
    14641 taaaatagtt attaacatgg gtataggtga cgctagagaa aatccaaaag gattagaaaa
    14701 aggcgttgaa gaattagaaa tgatatcagg acaaaagcct gttataacta aagctagaaa
    14761 atctgttgct aacttcaaat taagagaagg aatgccaata ggaataaaag ttacattaag
    14821 agctgacaaa atgttctact tcatggacaa attagtttca gtttctttac caagagttag
    14881 agactttaga ggagttaacc ctaatgcttt tgatggtaga ggaaattatg ctttaggagt
    14941 taaagaacaa ttaatattcc ctgaaataga atatgataaa atagataaag taagaggaat
    15001 ggatataata tttgttacta cagcaaaaac tgacgaagaa gctcgtgaat tattaaaatt
    15061 attaggaatg ccattttcta agtaagcaaa ggagggattc ccgtggctag aaaagcgatg
    15121 gttgttaagc aacaaagaaa gcaaaagtac gctactagag aatacactag atgtactata
    15181 tgcggaagac cgcattcagt tttgaaaaaa tttggtatat gccgtatatg ctttagagaa
    15241 ttagcttata aaggtcaaat acctggtgtt agaaaagcaa gctggtagaa gcttgattag
    15301 aatggaagga gggcaacaaa atgacaatga cagatccaat tgcagatatg cttactcgta
    15361 taagaaatgc taatgttgtt aagcatgaaa ctgtagatgt tccagcttct aatatgaaga
    15421 aagaattagc tagaatctta ttagaagaag gtttcataag aggttatgat gttatagaag
    15481 atggaaaaca aggtatcata agaatacaat taaagtacgg acaagaaggc gaaagagtta
    15541 taacaggtct taagaaaata tctaagcctg gaatgagagt ttacgctgct aaccatgaga
    15601 taccaaaagt attaaacgga ttaggaatat cagttatatc tacttcaaaa ggaatattaa
    15661 ctgacaagca agctagaaaa gaaaatgttg gtggagaagt aatctgctac gtttggtaat
    15721 taatatgaac aatgtaagga ggtgcgacca tgtcaagaat aggtgttaag ccaataataa
    15781 taccagcagg tgttgaagtt actatagctg aaggaaattt agttacagta aaaggtccaa
    15841 aaggaacttt aactaaacaa ttaagtgctg agttaaacat aaaaaaagaa gaaaatacaa
    15901 taatggttga aagaccaact gataataaaa aacatagatc tttacacgga ctaactagaa
    15961 ctttattaga taatatggtt gtaggtgtaa atactggttt cgaaaagaaa ttagaattaa
    16021 aaggtgttgg atatagagct caaaaacaag gaaagaaatt agttatgaac ttaggtttct
    16081 ctcatccagt tgagatggaa gatccagaag gaataactgt tgaagctcca aaccaaacag
    16141 agttaatagt aaaaggtata gacaagcaat tagtagggaa ttatgctgct aagataagag
    16201 cttggagaaa gccagagcca tacaaaggta aaggtataaa atacgttgat gaagtaatca
    16261 gacgtaaaga aggaaaaact ggtaaaaaat aataccaaaa cttaaacgct agaaaggagt
    16321 gagctaggtg ttaaaaaagg ctgataaaaa tgctaataga cttcaaagac ataagagagt
    16381 tcgtagaaaa ataagcggta cttctcaaag accaagatta tgtgtattta gaagtgctaa
    16441 caatatatat gctcaaataa tagatgacac aaaaagagta actctagttg ctgcatcttc
    16501 tttagaagca gaagttaaga gtgctgtcaa tcatacagga aacaaggaag cagctaagaa
    16561 agttggagaa ttagttgcta aaaaagctgt tgaaaaagga atcactgaag ttgtatttga
    16621 cagaggtgga tacttatatc atggaagaat tcaagaatta gctgaaggtg ctagagaagc
    16681 tggtcttaag ttctaatacg aaggagggaa aaatacatgc tacgtcgtaa gccgatagat
    16741 gcaggacaac ttgacttaca agaaaaagtt gttgaagtaa gacgtgttac taaggttgta
    16801 aaaggtggta gaaatttcag atttgcagca ttagtagttg ttggagatga aaacggacac
    16861 gttggtatag gtgctgggaa agcaatggaa gtaccagacg ctataaaaaa agcagttgaa
    16921 gatgctaaga aaaatctaat agtagtgcca atagttggga ctacaattcc tcacgaagta
    16981 cgcggacact ttggtgctgg aaatatatta ataatgcctg ctgtagaagg tacaggagta
    17041 atagctgggg gacctgctag agccgtactt gagctagctg gactaaaaga cgttagagct
    17101 aaatctttag ggtctaacaa tccaagaaat atggtaaatg ctacaataga aggacttaat
    17161 tctctaaaaa cagttgaaga tatagctaaa cttagaggta aaaaagttga agaacttcta
    17221 gggtaaggag gtagtgtaaa atggctaaat tacaaataaa gttagttaga agtgttatag
    17281 gaactactcc taaccaaaaa aagaatgttg aagcgttagg attaagaaaa agagaacaag
    17341 ttgttgtaaa agaagacaat gctcaaacaa gaggtatgat aaataaagta agtcacttat
    17401 tagaagtaac tgaaatagct gaataatttt tataaagtag tgcaaggagg tgcaatccat
    17461 gaagttacat gagttaaaac ctgctgaagg tgcagtaaga gctaagagaa gattaggtag
    17521 aggtactgca actggacaag gtaaaactgc aggacgtgga caaaaaggtc aatggtctcg
    17581 ttctggtggt ggagtaagag taggatttga aggtggacaa atgccactag ctagaagact
    17641 tcctaagaga ggtttcaata acatatttaa gaaagtttac acagaagtta atgttgaagt
    17701 tttaaacaga tttgaaaatg gaacagaaat aactgcagaa ttattaaaat ctactaaaac
    17761 tataagcaaa ataggtaaag acggaattaa aatcttaggt gaaggtaatt tagagaaagc
    17821 tttaactgtt aaagctgcta aatttacagc ttcagctcaa gaaaaaatag aaaaagctgg
    17881 tggaaaagca gagttagtat aataacctgc taatctccta gccagtacag gcggggtgaa
    17941 ttagcgtgct gtcaaaatta aaacaagctt ggaagattaa agcagtaaga aaaaaagtaa
    18001 tgtatacact tatgatgatc gtgatattca gaataggtac tactatacca gttccaggca
    18061 tagacactag tattatacaa aagatggtag gcggcaatag tctactttct ctgtataata
    18121 tgtttactgg tggagcattt agtaactttt cactatttgc tttaggtata agtccatata
    18181 tcactgcatc aatcataata caacttctaa cagtaggttt tgaaagttta gcagaacttc
    18241 aaaaatctgg tgaagaagga aagaaaaaaa tcaataagta cactaaatat acagcactag
    18301 ctctagcagt tgtacaagct ttgggaatta cattaggaat tgtaagaagt gctttaatat
    18361 caaatagtgt gttttttatc acaactgttg taataacatt agtttcagca agtatgttag
    18421 taatgtggat tggggacaaa attactgaaa aaggaatcgg taatggtagt tcagtaataa
    18481 tatttgcagg tataatatca agaataccta cagatgttat taaaatatcg caacaagtta
    18541 aatctggcga agttgcacct tgggttatag tgattctagc tgtagttata ttacttacag
    18601 taacaggagt tacatttata caagaagcaa ctagaaagat acctgtacaa tatgctaaaa
    18661 gagttgttgg aagaaaaatg tatggaggac aaagttctca tataccaatg aaagttaacc
    18721 agtcaggagt tatgcctata atttttgcta gttcactttt agctttccct caaacaatag
    18781 ctatgtttat gggtccaaat gctcaggctt ttgttcaaaa atacttaagt atggcaacag
    18841 aacaaggatt ttggacatat agatcaattg agattctact tataattttc ttctcatatt
    18901 tttatacaac tgtatcattt aatacagaag acatatctaa aaatatgaaa aataatggtg
    18961 gatttatacc aggtataaga ccaggtgagc ctactatgga ttatttaaat agaattttaa
    19021 ctagattaac tttagctgga gctactttct tagcaataat tgctatggtg ccagctctta
    19081 caacacatta tatgaaagta aatatgagtc tagctgggac ttcattgcta atcgttgttg
    19141 gagttgcttt ggagcttaag agacagttag agtcaaactt agttatgaga agctatcaag
    19201 gtttcttaaa ataaaatgga gatggataat atgagaataa tattacttgg acctccaggt
    19261 gctggtaaag gtactcaggc ggcaggaata gtagaaaaat acaatatacc tcatatatca
    19321 acaggagata tattcagaaa gaatataaaa gagggaacag aacttggaaa aaaagctaaa
    19381 gaatacatgg accaaggttt attagtacca gatgagttaa ctgtaggttt agttactgat
    19441 agaatatctc aagaagattg taaaaatgga tttatgttag atggatttcc aagaaatgta
    19501 gcacaaggag aacatttaga tatcttctta aaaaatgctg gtatatcact agataaagtt
    19561 gtcaatattg aagttgataa gagtatatta gtgtctagag cagttggtag aagaatatgt
    19621 aagtcttgtg gagctactta ccatgttgag tttaatcctc ctaaagtaga aggtgtatgt
    19681 gatgtatgcc aaggagaatt atatcaaaga gctgatgata atgaagaaac tgtatctaag
    19741 agaatacaag tttatctaga tgaaactaag cctttagtag attattatag caaacaaggt
    19801 ataatagcag atataaaagg tgatcaagca atagataaag tatttgaaga tattgtcgca
    19861 gccttaggaa gtggaaaata aatgattatc ttgaaatcaa aaaaagaaat agaactttta
    19921 agagaagcag gtaaaatagt tgctgacact catgaagttt taagaaaagc tatttctccg
    19981 ggaatatcta ctttagaatt agataaaata gctgaagaga atataagaaa atacaatgca
    20041 gaaccttctt ttaaaggata tggaggtttt ccaggaagta tatgtgcttc tatcaataga
    20101 gaggtagtac atggtattcc aggagaaact atattacaag aaggtgacat tgtaagtttg
    20161 gatataggag cctattataa aggatatcat ggtgattctg cgaaaactca cggagtaggc
    20221 atgatatctg aagaagatag gaaattgata gaagtaacta gagaaagctt ctatgaagga
    20281 ataaaatttg ctaaattagg atatagactt tcaaatatct cacacgcagt acaaacacac
    20341 gttgagaaac atggtttctc ggtagttaga gatttagtgg gtcatggagt tggagcaaat
    20401 cttcatgaag acccacaagt acctaattat ggactcccag gaaaaggacc aagacttaga
    20461 gaaggaatgg ttattgctat agagccaatg gttaatgcag gtcgttatca tgtaaagact
    20521 ctatcagatg ggtggacgac tgttacaata gatggaaaaa aatcagctca ttatgagcat
    20581 acgatagcaa ttactgaaca tgaacctttg atattgacaa agttgtaatt taaaaagtag
    20641 gtgaaaaaag tgctatcaga taatttaagt ataggtcaag ttgttaaagt ttcattagga
    20701 cgagataaag gaaatttatt ctttgtagtc aaaataatta ataatgaata tgtattaata
    20761 gctgatggaa aaaagagaaa acttgacaaa cctaagttaa agaaagtgaa gcacttaaaa
    20821 aaatataatt ttattaatga tgaagttaga aaaagagtag tatctggaca agagattaca
    20881 gattcttttt taagagctga acttactaag ttaaattaga taatttaact tattgaaatg
    20941 gagggctggt tagttaatgg ccaaaaaaga tgttatagaa ttagaaggta cagtttcaga
    21001 agctttacct aatgctatgt tcaaagttaa actagaaaat ggacatgaga tattatgcca
    21061 catttctgga aagctaagaa tgaacttcat aagaattctt gaaggtgata aggtgaatgt
    21121 tgaactttct ccatatgacc ttacaagagg aagaattact tggcgtaaga agtagactat
    21181 attaatattt agtctaagga gggattaata atgaaggtta gaccatcagt aaaaccaata
    21241 tgtgaaaaat gtaaagtaat aaaaagaaaa ggaaaagtaa tggttatctg tgaaaatcca
    21301 aagcataagc aaaagcaagg ataagaaatt attactagca ttttgttaaa ttttatagta
    21361 taatactata ttgtgatgtt aacaaaagaa cgttaaaatc gttgaacaat ctggcgtagg
    21421 attggcatat gatgtgaagt tttaaacact aaaaactatg tgtaggaggt gcgatcatgg
    21481 caagaatagc tggggtagat ttacctagag aaaaaagagc ggagataggc ttaacttata
    21541 tatatggtat aggtaaagca actgctaacg aaatattagc taaggctgag ataaatccag
    21601 atactagaat aaaagattta tcagaagatc aagttaatga gttaagaaaa gttatagatg
    21661 acgatttctt agttgaaggt gacttaagaa gagaaattgc tttaaacata aagagattaa
    21721 gagatataaa atgttataga ggaataagac acgctaaagg tcttccatta agaggacaaa
    21781 gaactaagac taatgctaga actagaaaag gtcctagaaa aacagtatct cgtaaaaaga
    21841 aaaaataaat aaataataac tagatgaagg agggaaaagg aaaatggcta aaccaaaaaa
    21901 gaaagttaca cgtattagaa gaagagaacg taaaaatata gaacgtggtc atgctcatat
    21961 acaatcaact ttcaataata caataataac tttaactgac gttcacggaa atgctatatc
    22021 ttgggcaagt tctggacaat taggattcaa aggatcaaga aaatcaactc catttgcatc
    22081 tcaaatggct gctgaaacag ctgcaaaagc tgcgatggaa cacggactaa aaagtgttga
    22141 ggtattcgta aaggggccag gttcaggaag agaagctgca ataagagctt tacaagcaac
    22201 tggactagaa gtaactatga taaaagacgt tactccaatc ccacataacg gatgcagacc
    22261 accaaagaga agaagagtgt aattaataag agtacaatag gaggtgtaga taaatggcaa
    22321 gatatacagg tgcatcatgt agacaatgcc gtagagaggg aatgaaatta ttccttaaag
    22381 gtgatagatg ttatacagac aaatgtgcta tagtaaaaag aaactatgct ccaggtcaac
    22441 atggccaagg aagaaagaaa gtttctaact atggattaca attaagagaa aaacaaaaag
    22501 ttaaaagaat atatggagtt ttagagactc aatttagaaa tttatatgaa cgtgctgaaa
    22561 atatgcctgg taaagcaggt gagaacttat taagtttatt agagagaaga ttagataatg
    22621 tagtttacag aatgggacta gcatcttcta gaaaagaagc tagacaatta gtgactcatg
    22681 gtcatttcac tttaaatgga aataaagtag acataccttc attaatagtt aaggttggag
    22741 atgttataga agttaaagag aaatcaagat cttctgcaaa attcaaaaat ttagtagaag
    22801 ttaactcaag aatagctcct aagtggttag aagctaatgt agaaggaatg acagcaaaag
    22861 tagttggtgt tccaacaaga gaagatatag atcttgagat agcagagcac ttaatcatag
    22921 aactttactc taaataataa gcacactttt aaaaattatt ttaaaatttt gtttaccctc
    22981 agtggattac tgaattttaa ggagggtttt gtccatgata gaaatagaaa agccaaaagt
    23041 agatatagtt gagcttagcg aagactatag atatggtaag tttgttatag aacctctaga
    23101 aagaggatat ggaataacta taggtaacgc attaagaaga atattattat catcattacc
    23161 aggtgtagca gtgaatgcta taaaaataga tggagttctt catgaatttt caacaatacc
    23221 tggtgttaaa gaagatgtta ctgagataat attaacttta aaagagcttt cagcaactat
    23281 agatggtgaa ggaagtagaa cacttaaaat agaagctcaa ggaccatgct ctatcacagg
    23341 tgcagatata atctgtcctc cagatgttga aatattaagt aaagatttag caatagctac
    23401 attagatgat aatgctaaac ttaatatgga gatatttgta gataaaggta gaggttatgt
    23461 ttctgctgaa gaaaataaaa cagagaatgt tccaataggt gttttacctg tggattcaat
    23521 atatactcct gttgagaagg ttagttatca tgtggaaaac acaagagttg gtcaaaaaac
    23581 agattatgat aaattagtac tagaagtttg gactaatggt agtataaatc ctcaagaggg
    23641 aatatcatta gctgcaaagg tattagttga gcatttaaac ttatttatag acttaactga
    23701 acatgtaagt agtgttgaaa tcatggtaga aaaagaagaa gatcaaaaag aaaaagttct
    23761 tgaaatgact atagaagaat tagatttatc agttagatca tataactgtt taaagagagc
    23821 gggaattaat acagttgaag aattagctaa taaatctgag gatgatatga tgaaggttag
    23881 aaacctaggt aagaagtcat tagaagaggt aatacaaaaa ctagaagaac ttggattggg
    23941 attaaaacca agtgaagaat agcgagcaaa ggagggataa gaatggctaa gtaccgtaaa
    24001 ttaggacgtg aaacagctca cagaaacctt atgttaagaa acttagtaac ttgcttacta
    24061 agaagtggaa gaatagaaac tacagtgact agagcaaaag aaactcgtag aatggctgaa
    24121 aagatgataa ctcttgctaa gagaggagat cttcatgcta gaagacaagt tttagcttat
    24181 gttatggatg aaacagtagt taataactta ttcacagatt tagctccaaa atatgctgag
    24241 agaaatggtg gatacactag aataataaaa atagggccaa gaaaaggcga tgctgctgaa
    24301 atggctttta tagaattagt atagtaagga aaggattagg cttgatggct taatcttttt
    24361 tttatttttt tgactaaggt atgatataat aatgtgtaaa atgattttta taagttattg
    24421 cttactaaaa ggagtatgag ttaatggata atatagtaaa ggtaaataat atttcgtttg
    24481 agtatattac agatgaagca aaacttaaag caatagataa tttaagccta gatgttaaaa
    24541 aaggagaatt tgttgcgata ataggacata atggttctgg taagtctact ttatctaaaa
    24601 acttaaatgc tattcttatg cccactgaag gaaatattct tattgatgat atggatacta
    24661 aagaagaaga aagattatgg gatataagac aaactgcagg catggtattt caaaatccag
    24721 ataatcaaat agttgctact atagtagaag aagacgttgc atttggacct gagaacttag
    24781 gtatagaacc aaaagaaata agacgaattg tagaggagtc tttaaaaagt gtaggtatgt
    24841 atgatttaag agatagacag cctcatttgt tatcaggagg tcaaaagcaa agagttgcaa
    24901 tagcaggaat tattgctatg aggcctaaat gtataatctt tgatgaagca acagcaatgt
    24961 tagatccatc tggaagaaaa gaagttatga aaacaataaa aagacttaat aaggaagaaa
    25021 atataactgt tatacatata acgcacttta tggaagaagc agtagaagct gatagagttg
    25081 tagttatgga aaaaggaaag aaaatcttag agggaactcc tagagaagta ttcagtaaaa
    25141 taaagatgtt aaaggaaatc ggtttagatg taccttgtat gacagagtta tctagtttat
    25201 taatagaaga aggtataaat attagtagtg atatattaac tgtggatgag atggtgatgg
    25261 aattatgtca attatagtaa agaatttaac acatatatat aatgaaggga tgccttttgc
    25321 aagtaaggca cttgatgatg tttcttttga gattaaagat agagattttg ttggtcttat
    25381 aggtcataca ggctcaggaa aatctacatt aatacaacat ttaaatggat tactaaagcc
    25441 ttcctcagga gaaatattta taaatgattt taatataaca gataaaaact tgaatttaac
    25501 tgagattaga aaaagagtag gggttgtatt tcaatatcca gaataccaat tgtttgaaga
    25561 aacgattgat aaagatatag cttttggacc ctctaattta ggcttagagg agtctgagat
    25621 acataacagg gtaaaagcat caatggaagc tgtaggactt gattatgagg tatttaaaga
    25681 caaatctcct tttgaattat ctgggggaca aaagcgtaga gtagcaattg ctggagttat
    25741 agccatgaac ccagaagttc tcatattgga tgaaccaact gctggtcttg accctggtgg
    25801 aagagatgaa atttttaact taataaaaga cttacatgaa aaaaagaata tgactataat
    25861 actatcatca catagtatgg atgatatggc aaagttagca aaaactttaa ttgttatgaa
    25921 tcatggtagc gtagaattta tgggaactcc tagagaagtg tttaaatcaa atgctagtaa
    25981 attaaaagat ataggattag atattccaca agttttagag cttgcattga aattgagaga
    26041 aaaaggcttt gacatcagtg aggatatatt gactttagaa gaagcaaaac aggaaatatt
    26101 aaaagttgtg agaggacgag gattatgtta aaagatataa ctatagggca atactaccca
    26161 acaagttctg ctattcataa attagatcca aggataaagc ttgtagcgac aattgtattt
    26221 atggtatcta tatttgtagt aaataaattt tggccttata tagtagtttt attatgttta
    26281 ttagctatga taaaattggc taatatacca gtcaaatata ttgttaaggg tgttaaacct
    26341 cttaaatgga ttattttgtt tacatttctt ataaatattt ttttcttacc aggtgatgaa
    26401 atatggtcat ttggattttt agctattaca aaacagggtc taagacaagc aatttttatg
    26461 gcaataagat taatattttt agtagttggt acatcattgc ttactttaac aacttcacca
    26521 atagaattaa ctgatggaat agagagattg ttgaatccat ttaagaaaat tggtttgcca
    26581 gttcatgaac ttgcaatgat gatgacaatt gctttacgtt ttattcctac attgttagat
    26641 gagacagaca aaataatgaa ggctcagatg tctagaggtg cagattttga aagtaagaat
    26701 cttataaata gagcaaagaa tttagtacca cttttggtac ctttatttgt aagtgcattt
    26761 agaagagctg atgaattggc tatggccatg gaggccagat gttacagagg tggacataat
    26821 aggactaaga tgagagagtc tgttatatca agaagggatt atatggcatg tgtatttcag
    26881 gttgtttatc tgggagcaat tatagctact agatttatag caatttaatt aataagttat
    26941 agatataatg aggtaaagta tgagaaatat aaaaattaaa atccaataca gtggtaagaa
    27001 ttattgtgga tggcaaaagc aacctgattc tttagggata caaggtacaa ttgaaagggc
    27061 tatatatgat ataacaaaag aagaaaccag tcttataggg tctggtagaa ctgattcagg
    27121 agttcatgct attggtcaga ttgcaaattt taaaattaat tcaggtataa gtatagaaag
    27181 tataccaatg gcactaaatg ccaagttgcc aaaagacata tcagttatag aagcatgtga
    27241 agtaaatgat gattttcact ccagatacag tgcaaaaggt aaaacatata aatatttagt
    27301 atataatagc aagtttagaa atccaatact tagtgagata tcatatcaag taaaatatga
    27361 acttgatttt gataaaatgt gttcagaagc aaaaagttta ttaggaacac atgattttaa
    27421 aggttttatg agttctggtt catctgtaaa agatacagtt agaacgatat atgatattga
    27481 tataagtaaa aaagatgact tgattacttt tgagattagt ggtaatggtt ttttatataa
    27541 catggtgagg attattgttg gaacacttgt tgatatgggt cgtggcagaa ttaacgaacc
    27601 atttttagat ataatacaat caaaaactag gtcaagatgt ggacatacag ctccagcaca
    27661 aggattattt ctcaaaaaag ttcattatta acttgacaca ccaggctaca tgtattaaaa
    27721 tatatgagaa tgtgttaata agtccacttg ccccggactt gacataaacg gtaaaaaaat
    27781 tttatgaaaa gtagttaagg agggacagtt atgaaaagtt atatagctaa gccagctgat
    27841 gtacaaagaa aatggtactt agttgatgct gaaggaaaaa cattaggtcg tttagcaaca
    27901 gaaattgcga cagtattgag aggtaaacat aaaccaacat ttactcctca cgttgatggt
    27961 ggagattttg ttgttgtagt aaatgcagaa aaaatagttt taagtggtaa gaaattagat
    28021 caaaaatatt acagatacca tactggttat gtaggtggat taaaagaaat atcttacaga
    28081 gatatgatgg ataagaaacc agaagaagtt atatcacatg ctgtaagcgg tatgttacct
    28141 aaaaataaat taagaagcag aatgatgact agattaagag tatttgcagg tgctgagcac
    28201 actcatgcag ctcaaaatcc agaagtttta aactttaaat aagattaata ggtgaaagga
    28261 ggagtttata atggctaacg ttcaatatta tggaactgga agaagaaaaa gttctgttgc
    28321 tagagtaaga ctagttgcag gtgaaggaaa tatattagta aatggaagag cattagaaaa
    28381 ttattttaat tatgagacat taataagaga tgttaaacaa ccattagttt taactggtaa
    28441 tgaaaataaa tatgatgtta tagtaaaagt tgaaggtgga ggattcactg gacaagctgg
    28501 agctataaga catggaatat ctagagcttt attaaaagct gatttagatt taagacctgc
    28561 tttaaagaaa gaaggtttct taactagaga tgcaagaatg aaggaaagaa agaaatacgg
    28621 attaaaagca gcaagaagag ctccacaatt ctcaaaaaga taattactta tttttttgga
    28681 aaatcaaaat cctttattta ctgttattca gaaagtattg gtattttaaa aatgttgggt
    28741 taagttaatt attgatatat aataaaataa taagatatct tattaggatg tttttcatta
    28801 agaagaacat cctttttatt ttgactattt aataaaaaac atatgctaat aacatttaaa
    28861 ttttattttt ataatattgt aatttaaaag tttatataag gttatatatt ttaagatatt
    28921 atttaacaat ataaattctg aatatacgtt atcaaagttt ataatataaa ctaagtataa
    28981 tttattgtat agtattgaat gttagataaa catagtgaat taaatagggg cagaaatata
    29041 atatcattac ataataatat attaatacca ataaataaag atatatcaat attaaaaaaa
    29101 tttaaatgtt tgtactaata tatggaatat tatagtgttt ttataagtat acatatacag
    29161 ggtgatgttg tgagaaagta cataaaacat ataattttta gttttgcaat gatatgtcta
    29221 gtagtagtat ccatatttga aattaaaaat atttctgaag atgttatcaa gtatatgcca
    29281 gtaacaaaca aaactataat tttagatgct ggtcatggag gcattgatcc aggtgcatta
    29341 aataaggata agagtacatc tgaaaaggat attaacctag caataacact taagcttaga
    29401 gagcttatag aatcaagtgg tggccttgta atattaactc gagaagatga cagtagcctt
    29461 tataaagaag aaaataataa aactacaaga caaaaatata atgagaattt gaaaaataga
    29521 aaagaaataa tatctaattc taatgccaat atgtttgtct ctatacatct taatgcattt
    29581 gaacaatcaa aatactatgg agctcaaact ttttatccta aagacaaaca agacagtaaa
    29641 gaattatcga agtgtattca agaagaactt aaaagagtag tagataagac aaacaacaga
    29701 gaagtcaaac caagggatga tatatatctt ttgaaagaaa ataatattcc atcagtactg
    29761 atagaatgtg gttttttatc aaatgaaaaa gagtgtaaac tcttaactga tgaaacatat
    29821 caagaaaaaa tagcatgggc aatctacata ggaatacaaa aatatttaag ttgaaataac
    29881 tataaaaata ggtctaaata tgtaaatagc ttgtattaaa gcagttaaaa tgcattaata
    29941 taggctattt ttattttgac aaaaaaatat ttaaaataaa agttaaaaag ttgttgactt
    30001 agaataatat agatgatatt atatatgagt gcccaaaagg agcaccaaaa taagac
//
LOCUS       DAEMHW010000031        29642 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEMHW010000031 DAEMHW010000000
VERSION     DAEMHW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.031"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            37..1488
                     /locus_tag="KPK67_001183"
     CDS             37..1488
                     /locus_tag="KPK67_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG2458178.1"
                     /translation="MDFFNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1708..2169)
                     /locus_tag="KPK67_001184"
     CDS             complement(1708..2169)
                     /locus_tag="KPK67_001184"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBG2458179.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(2274..3467)
                     /locus_tag="KPK67_001185"
     CDS             complement(2274..3467)
                     /locus_tag="KPK67_001185"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBG2458180.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDAYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(3490..4155)
                     /locus_tag="KPK67_001186"
     CDS             complement(3490..4155)
                     /locus_tag="KPK67_001186"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBG2458181.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(4189..5301)
                     /gene="ribD"
                     /locus_tag="KPK67_001187"
     CDS             complement(4189..5301)
                     /gene="ribD"
                     /locus_tag="KPK67_001187"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBG2458182.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(5433..5554)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5743..8226
                     /gene="recQ"
                     /locus_tag="KPK67_001188"
     CDS             5743..8226
                     /gene="recQ"
                     /locus_tag="KPK67_001188"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBG2458183.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      8315..8426
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8568..9869
                     /gene="thiC"
                     /locus_tag="KPK67_001189"
     CDS             8568..9869
                     /gene="thiC"
                     /locus_tag="KPK67_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBG2458184.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            9923..10117
                     /gene="thiS"
                     /locus_tag="KPK67_001190"
     CDS             9923..10117
                     /gene="thiS"
                     /locus_tag="KPK67_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBG2458185.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            10110..10913
                     /gene="thiF"
                     /locus_tag="KPK67_001191"
     CDS             10110..10913
                     /gene="thiF"
                     /locus_tag="KPK67_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBG2458186.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDALESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDIIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            10976..11746
                     /locus_tag="KPK67_001192"
     CDS             10976..11746
                     /locus_tag="KPK67_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBG2458187.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMKILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAASVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            11763..12866
                     /gene="thiH"
                     /locus_tag="KPK67_001193"
     CDS             11763..12866
                     /gene="thiH"
                     /locus_tag="KPK67_001193"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBG2458188.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRETLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            12867..13556
                     /locus_tag="KPK67_001194"
     CDS             12867..13556
                     /locus_tag="KPK67_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG2458189.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELKK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            13744..15138
                     /gene="yfcC"
                     /locus_tag="KPK67_001195"
     CDS             13744..15138
                     /gene="yfcC"
                     /locus_tag="KPK67_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBG2458190.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            15654..17021
                     /locus_tag="KPK67_001196"
     CDS             15654..17021
                     /locus_tag="KPK67_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBG2458191.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKIDYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            17046..17654
                     /locus_tag="KPK67_001197"
     CDS             17046..17654
                     /locus_tag="KPK67_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBG2458192.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            17702..18073
                     /locus_tag="KPK67_001198"
     CDS             17702..18073
                     /locus_tag="KPK67_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBG2458193.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            18252..18590
                     /locus_tag="KPK67_001199"
     CDS             18252..18590
                     /locus_tag="KPK67_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBG2458194.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            19057..19560
                     /locus_tag="KPK67_001200"
     CDS             19057..19560
                     /locus_tag="KPK67_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBG2458195.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            19607..20566
                     /gene="moaA"
                     /locus_tag="KPK67_001201"
     CDS             19607..20566
                     /gene="moaA"
                     /locus_tag="KPK67_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBG2458196.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            20570..21040
                     /gene="moaC"
                     /locus_tag="KPK67_001202"
     CDS             20570..21040
                     /gene="moaC"
                     /locus_tag="KPK67_001202"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBG2458197.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            21076..21507
                     /locus_tag="KPK67_001203"
     CDS             21076..21507
                     /locus_tag="KPK67_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBG2458198.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
     misc_binding    21937..22148
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            22311..23600
                     /locus_tag="KPK67_001204"
     CDS             22311..23600
                     /locus_tag="KPK67_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG2458199.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            23654..24733
                     /locus_tag="KPK67_001205"
     CDS             23654..24733
                     /locus_tag="KPK67_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBG2458200.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNKKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAIENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            24754..26298
                     /locus_tag="KPK67_001206"
     CDS             24754..26298
                     /locus_tag="KPK67_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBG2458201.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFVDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(26461..27003)
                     /locus_tag="KPK67_001207"
     CDS             complement(26461..27003)
                     /locus_tag="KPK67_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBG2458202.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(27151..27435)
                     /locus_tag="KPK67_001208"
     CDS             complement(27151..27435)
                     /locus_tag="KPK67_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2458203.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(27498..28262)
                     /locus_tag="KPK67_001209"
     CDS             complement(27498..28262)
                     /locus_tag="KPK67_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG2458204.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(28544..28810)
                     /locus_tag="KPK67_001210"
     CDS             complement(28544..28810)
                     /locus_tag="KPK67_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG2458205.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(28852..29606)
                     /locus_tag="KPK67_001211"
                     /pseudo
     CDS             complement(28852..29606)
                     /locus_tag="KPK67_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT        11149 a         3338 c         5042 g        10113 t
ORIGIN      
        1 gagaaaacaa aactaaatag gggggtaatt aatataatgg attttttcaa tggtatttta
       61 acgagtatta acaatgcagt agtatttgta aacaattact tatggtcgta tatcttaatt
      121 ataatgttag ttgtaattgg tatttacttt actattaaaa caaattttgt acaattcaga
      181 tattttatag aaatgtttag acttctgggt gatggtacag cgaataaaaa tgcaaaaaag
      241 gaaggaaaaa tctcttcatt tcaagcattt tgtataagta catcttcaag agttggaact
      301 ggaaatatag ctggaatagc aattgcagtg gtagcaggag gacctggtgc tgtattttgg
      361 atgtggctta tagcattaat tggttctgca tcaagctttg ttgagagtac attagctcag
      421 atttacaaag tgaaagatgg tcaagctttt agaggtggac cagcgtatta tatggaacaa
      481 ggtctaaata aaaaatggat gggtatttta ttctcaatac ttataactat atgttatggt
      541 tttgtattta atgcagttca agcaaatact gtttcacttg catttaataa tgcatttgga
      601 ataagtaaaa tggctatggg aataggtctt ggaattttaa ctgcaatagt tatttttggt
      661 ggagtgcata gagttgcaaa agtttctgaa ataatagttc ctgtatttgc aggattatat
      721 atattagttg ctttggctat agttgtaatg aatataacag agataccaag tgttatagca
      781 ttgatagttg aaagtgcatt tgattttaaa ggaatggcga ttggtacatt tatgggagtt
      841 gtaatgactg gagtgaagag aggtctattt tctaatgaag caggtatggg tagtgctcct
      901 aatgctgctg caacagcaca tgtaactcat cctgtaaaac aaggtttaat tcaatcttta
      961 ggtgttttta cagatactat aataatatgt agttgtacag cattcatggt attattatat
     1021 tcaggttata gtagtagtgg agcaactgga attgaattag ctcaagaagc tttaactcat
     1081 catataggac cagttggaaa tatatttata gcagtttgta tattcttgtt tgcatttagt
     1141 tctatagttg gtaattacta ttatggtgaa tcaaatatgg agtttatgag tggaagtaag
     1201 actaaactta atgtatttag agtttttgtt gtaggtatgg ttttatttgg ttcacttact
     1261 caagtagatg tagtttggaa tcttgcagac ttatttatgg cattgatggc gataataaac
     1321 ttaatagcaa tagcactttt aggaaaatat gcatttattg ctttaaaaga ttatacaagt
     1381 caaaaaaaat caggaataaa agaccctgtg tttattgcag atgaaataga aggcctagaa
     1441 aaggtatcag agtggcatag ggaattatct gaggaacatt taggataaaa aatagtataa
     1501 aattataaaa ccattttgaa agactagagt atgtatatac tttatatgaa tataatattg
     1561 aattaattta tattactgga atttttaatt ccagtaatat ttttttatta tataatttag
     1621 ctttattaaa agtttttatt attaaaaata gcttatttat cgtaaagtga atgatattgg
     1681 gtttttaatt attatgaatg ttagtattta atttaaagac ttaaataaat ttgccatttc
     1741 tagcgcattc atagcacaat cataaccttt atttccagct tttgtacctg ctctttcaat
     1801 agcttgttca atattttctg tagttacgat tccaaatata acaggtaatt cactgtctaa
     1861 agatactttg gctatccctt tagagacttc actacataca taatcataat gagaagtagc
     1921 ccctcttata acacagccta aacagattat agcatcatac ttaccacttt tagccatttt
     1981 ttgagcaaca agaggaatct caaaagctcc aggtacccaa actatttcta tattttctgg
     2041 agaaacgtca tgtcttaaaa gacagtcttc agctcctgat aaaagttttg atgttataaa
     2101 ttcattaaaa cgagaattta ttatacctat ttttaaatct tttccaataa gttttccttc
     2161 atatatcatt tttatttcct ccaattttat ttatgttata gatttatatt tataaatatc
     2221 tttaatttaa ttataaaaaa ctacttagta gaaaaatatc atcatataat catttatata
     2281 atatctaaaa gatgtcccat tttatctttt ttagttttta aatatagctt attaaagtca
     2341 ttaatttctt cattaatagg gattctattt tcgattttta ttccatattt ctctaattga
     2401 tttattttat ctgggttatt acttaaaaga tttatacttt ttatgtttaa attttttaat
     2461 atttgagcag ccatatagaa atctctcata tcttcttcaa atccaagcat taaatttgct
     2521 tcaacagtat cgtaaccttc ttcttgaagt ttataagcct taattttatt taaaagacca
     2581 atacctctac cttcttgctt catataaatt aaaactccac ttccattttc cacaattttc
     2641 ttcatagaag attctagctg taatccacaa tcacatttta gagagtggaa cacatctcct
     2701 gttaaacatt cagaatgaag tctaactaga gtattttcta tatttatatt tccataagta
     2761 agggcaatgt gttcttcatt tgaataagca tctctatatc ctatgatttc aaaatttcca
     2821 tattttgtcg gaaggaaagc actagatact ttttctatag tagtttcatt tattttgcgg
     2881 tattctataa ggtcttttat agtgatgatt tttaaattat gcttttcttt gaaaatcatt
     2941 aaatcatcaa ctcttgccat tgtaccatca tctttcatta tttcacagat aagaccaatc
     3001 tctttaaaac ctgcaagtct agcaaggtct actgaggctt ctgtatgtcc atccctgaca
     3061 agaactccac cagtttttgc tattaaagga aagatgtgac caggcgtttt gaaatcttta
     3121 cttgtagaat tttcatcaca gaacaatttt acagtttcag ctctttcata tgcagatata
     3181 ccagttagag tatctgctcc atctatagta acagtaaagg ctgtttgaaa tgtatctgta
     3241 ttttctctaa ccataagagg tatattaaga cttttaaatt ttttttcagt ggcaggtaga
     3301 caaattagac ctctaccata tgtagccata aaatttattg attcaggagt agccatttca
     3361 gctgccatca ataaatctcc ttcattctct ctactttcat catcaactac gattaccatt
     3421 ttcccatttt ttatatcttc aatagcttct tcaatagtat taaacatgta attcactcct
     3481 ttattatttt taaaaaccat ttttaaataa aaaatcttta tctatatttg atttatggtt
     3541 actactatta gaatttaatt ccttgtagtt attagccata aaattattaa catactttcc
     3601 aattacatcg ctttctaaat taacgaaatc tccaacattc tttgtcaaga gaatagtgtt
     3661 aattttagta tgaggtataa tagaaacctt aaatatttca tcatctaaat atgatatagt
     3721 taaacttaca ccatcaatag caacagaacc tttgagtatc attgaggata aaagtttttt
     3781 atcaggtcgt atacttacta aaactgcatt ttcatctttt tcaaatttgc agatagttcc
     3841 tttaccatca acatgacctg ttactatatg accaccaaat cttgaagaaa gactcatagc
     3901 tctttcaaga tttaccttgt catttgcatt taataaacct aaatttgtgg aacgaattgt
     3961 ctccatcata acatcggctg taaattcatt ttttccgagg tttgaaacag ttagacaaac
     4021 tccatttacg gctatactat ctccaagatt tgtgccatct aatattttat ttgatagtat
     4081 ggttactttt ccactcttac cattagcagt aatttttcta agtattccaa cttcttctac
     4141 tatgccagta aacatcctaa cctcctatat gaatgtatta acaaaaattt aatttaaaac
     4201 atctgattct ataagaatat cgccatctat caaagttaca ttattgattg ttaactgtgt
     4261 tgaatctgac aaatgattta ttccttctcc agaaacgaat gtaggtgcat ctttaccacc
     4321 tataatcttg ggtgatagaa ataatttgac tttatcaact aacttatttc taaagagact
     4381 gtcattgaga gtagcaccac cttcaactag aacagaatca atgtttaact ctcctaattt
     4441 atacattaca aactccaaat ctactaagtt gttttttgaa ggagaaatta ttgtttcaac
     4501 attattttct tttaacatag ataattttat actttctttg caacatgtga aaactatagt
     4561 tttactggtt ttatctttaa ccaaattact attaagtggc aactttaggt gtgtatctat
     4621 tactattctt gtaggatgag acactttttc ttgattgagc ctacaagtta gtaaaggatt
     4681 atcgttcaaa actgtattta tgccaaccat tatactttgg tatttatttc tgtatttatg
     4741 agttagatac cttgatgatt cattagatat ccattttgat tctagtgatt ttgtggcaat
     4801 tttaccatct agacttactg ctgattttac aatacaaaga ggtcttttgt tttttatatg
     4861 ataaaaaaat gcctcattta aatctcttgc ttcatcttct aatattccca cagtaacatc
     4921 aatattgtta tcttttaatt tttttactcc attaccacaa acaagtggat ttgggtctaa
     4981 tgtagatatt acaactctct taatcttatt ttgtataatt ttatctacac aaggaggcgt
     5041 tttcccataa tgagaacatg gttctaagtt tacataaaga gtagagtctt ttagactttg
     5101 tttagcactg tttatagcat ttacttcagc atgattagag ccaaatttct catgatatcc
     5161 tttgcctatt atattactat ctttaacaat tacacaccca actaatggat ttggatttac
     5221 aaagccttct ccatttttag ccaattcaat ggcttttttc atataataaa tatctttttc
     5281 tttttgattc acaaaattca caatatgtac acctcctatt taattggata atttttaaac
     5341 aaaaaaccct gagaattaac tcagggttta gaataatcta caataaatgt ttaataaatg
     5401 tagaacaaaa ttgctctaca aaaacactca ttaaatatct tcttccatcc agactttact
     5461 gtcggctttg gaatttaacc aaatcaacca agttttacct tggctcgcgg gctataccgc
     5521 cgattgggaa tttcacccta ccccgaagat taagtattaa atttatattt agaatgtatc
     5581 ataaattatt ttactttgca aactattttt ttaaaaaata tatgttaata tataatatag
     5641 aatgtctata aactttgaaa taccacgatt gtgaatataa aaataaaaat ttgaacataa
     5701 tcaggaaaga agtctaatta atttaaatta ggagagtaat atatgaataa aaaactttta
     5761 gaaatactat caaaatacta tggatataca agttttagaa aaggtcagga atctataata
     5821 aattctatct taagcgaaaa agatgtgtta gctattatgc caacaggagg gggaaaatca
     5881 atatgttatc aaattccagc tttgatttta gatggaatga caatagtaat ttctccactt
     5941 atatcactta tgaaagacca agtagatgcc ttaaagacta tgggaattag tgctgctttt
     6001 ataaatagtt cattaagcag caaagaattt aatgaaatat taaataatat tagaaaaaat
     6061 aattataaaa tactctatat tgctccagag agattagatg cacaagagtt tattgagcta
     6121 ataaataata acaatgtaag tcaagttgcg atagatgaag cacattgtgt atctcaatgg
     6181 gggcatgatt ttaggttgag ttatagaagg atttctgatt ttataaaaaa tttgcctaaa
     6241 agacctatag ttacagcatt tactgcaaca gcttcagaag aggtaagaac tgatataata
     6301 aatttactgt gtttagaaaa tccagattat tatataactg gatttgatag agagaattta
     6361 tcaataaata tagtaaaatc atctagtaaa aataagtata ttttagatta tattcaaaat
     6421 cataaaaatg agtctggaat tatatatgtt gcaactagaa aggaagttga aaatatatat
     6481 aatggtttat ctaagaggaa tatatctgta tcaaaatatc atgcaggttt atctaaaaat
     6541 gaaagaagta aaaatcaaga agattttata aatgataatg tggatataat ggtagcaaca
     6601 aatgcatttg gaatgggtat tgataaacct aatataagat gggttataca ttacaatatg
     6661 cctcaaagta tagaaaacta ctatcaagaa ataggtagag ctggtaggga tggagaaaaa
     6721 agtgagtgtg tacttttatt ttctccagga gatgtacata tacaaaaata tttaattgat
     6781 attggagttg aaaatcctga aagaaaatta tttcagcata agaagcttca atacatgatt
     6841 gacttagttt acagtaattc atgctataga aagacaatat tgagttattt tggagaaaat
     6901 tatatagaca attgcaataa ttgtagcaat tgtttagtag agggtgaaat agtagataaa
     6961 actatagatg cacaaaaagt catttcatgt gtagctagaa tgaaaagaag ttatggtgtt
     7021 actactattg tagatgtgct tagaggttct aagaataaaa agattttaca gcttggatta
     7081 aatactttgt ctacatataa cattatgaga gattattcta gtgaagactt aaagaatttt
     7141 gtaaatacat tggtttctca tggtttttta gatttagttg agactctagg caatggaaat
     7201 agaggtagtt ttcctactat aagattaaat gagatgtcta tgaaagtttt aaaaggtgaa
     7261 gtgaaagtag agtttaaaga aattgttatg acaaaatcat tggaagtaga ggatgagtta
     7321 tataatgctt taagagaact tagacattca attgcaagtg aagaaaggat agctccatat
     7381 atggtatttg gagatggtac acttagagcc atgtctagta gttatccaat taataaagaa
     7441 gaaatgctag atatctctgg agtaggagaa attaaatatc aaaaatatgg tagagatttt
     7501 gaagcagtaa ttaaagaata tgtcgaaaaa aataatataa ataaaaaaat agatgaaaaa
     7561 aatgtcgatt caaagaacgt taatagtgat ttcttagagg ttaatacaga taaagtttta
     7621 tatgaaaaac tgttagtcat tagagaagaa tatgcaacaa aggaaaaatt acctgcatat
     7681 atgattttag ccaataaggc tttgaaagaa ataagtggaa gatatccact tgatgaagaa
     7741 caattaaaag atatttctgg cataggtgga gtaaaatttg aaaaatatgg agctagtatt
     7801 ttggcagagg ttaataaata tgtaaaagaa aataatatta atgttatttg ggagaaaaag
     7861 ggcaaaagga agttaatatt agatggagaa agtagaaaaa ataatgaaat agcattagat
     7921 ttgctaaatc aaggaaaaga tatacaaagt atcagtcaag aaatagaagt ttcactgtca
     7981 accattattg gatatgtgca tgactatgta aaggatggac atagtatatc atttgaatta
     8041 ggactagaaa atttttataa tgaagataaa aagaagatga ttttagaagt ttgcgaaaaa
     8101 gttggctatg ataatttaaa taacataaaa agaaaacttc cagattcaat aaagtatgaa
     8161 aatataagag ctgtgatact tgactcttat cttgaaaact taaaaaataa tattatagca
     8221 gagtaatttt taacttaata aaaaataaaa tttgtattat tgtgttgaac taataatttt
     8281 gctgtgttat actgaatttg taaaaataat gaaaaattag agctaggggc gctagaattg
     8341 gctagctgag agataaagcc gtaacttaca aaccctaata acctgatcaa gataatgctt
     8401 gcgaagggaa gctttttaat ataattttat tatataaata ggcaaattat tcaatgttag
     8461 gagaaatttg tctaagtgta tatacgatta taagagtgct ttccaaacga ggaaaagcac
     8521 tttttttatt ttcaaaaact ataactttaa tcttggagga ttgtaatatg aactatacaa
     8581 cacaaatgga tgctgctaga aaaggcataa taactaaaga aatggaaatc gtttctcaaa
     8641 aagaacaggt agatgtaaat gaattaagag aattaatagc aaatggacaa gttgttatac
     8701 cagctaataa aaatcataaa tctttaagtg cagaaggtgt aggtaaaaat ttaagaacaa
     8761 aaataaatgt aaatctagga atttcaagag attgtaagga tatagaaaaa gaacttgaaa
     8821 aagttagagt agcaatagac atgaaagctg aagctataat ggatttaagt aattatggca
     8881 aaactagaga atttagagaa aaggttgttg aaatgtcacc tgcaatgata ggttcagtac
     8941 ctatgtatga tgcagtagga tatttggaaa aagaattaaa agatataaca gaagaagagt
     9001 ttttaaatgt aataagacaa catgctattg atggagtaga ctttattact atacatgcag
     9061 gtcttacaag aagtgtttgc caaaaaataa aaaatcatga gagattgact catatagtat
     9121 ctcgtggagg ttctttattg tttgcatgga tggaattaaa taataaggaa aatcctattt
     9181 atactaattt tgataaaata ttggatatat gtgaagaata tgatgttaca ttgagtttag
     9241 gagatgcttg tagaccaggt tgtataaaag attctactga tggtgttcaa attcaagaac
     9301 ttgtagtatt aggtgaactt actaaaagag catgggaaag aaatgtacaa gtaatgatag
     9361 aaggtcctgg acacatggct attgatgaaa tagaagcaaa tgtagtatta gaaaagagat
     9421 tatgccatgg agcacctttt tatgttcttg gaccattggt tacagatata gcaccaggtt
     9481 atgaccatat aacaagtgca ataggaggag ctttagcatg tgctaaagga gtagattttt
     9541 tatgttatgt aactccagca gaacatttaa gattgcctaa tttagatgat atgaaagagg
     9601 gtataatagc tgcaaagata gctgctcatg caggagatat agctaaaaat gttaaaggtg
     9661 ctcgtgaatg ggataataag atgagtaaag ctagagctga tttagactgg tgtgagatgt
     9721 ttagacttgc aatagaccct gaaaaagcta agagatacag agatgaatca actcctactc
     9781 atgaggatag ttgtactatg tgtggaaaaa tgtgctcaat gagaacagtt aagaaaatat
     9841 taaacaatga ggaacttaat ctaatataat ttttggctag tatgactgga ataaaaagaa
     9901 gctgaaaaga ggaggtgatt tagtgaaggt taatgggaag gaaattgaat ttgaaaaaga
     9961 tttgactgtt atagatttat taaataaata taatttaaaa agtgatagag ttgtagttga
    10021 agtgaattta gagataattg aagaatctaa ctacaataca tatgtattaa aagacgagga
    10081 cattgtagag cttattagct ttattggagg tggttaaaat aaaaatattt gtaaatgaat
    10141 tattaacaga tgttgaggaa aatacaactg cttataaagt tagagatagc tataacaata
    10201 aatgtgatgt attggtgtta aatggatatc caattaaaac agatatgccc ctttgtgaaa
    10261 atgataaatt gacacttata caaaaaggag ttaagccatc acttgatgca ttggaaagct
    10321 tgttgattgc tagacatact ccaaatatac ataataaatt aaaaaaaggg aaagttgcta
    10381 tattgggatt ggggggactt ggttccaata tagctatctc tttagctaga ataggagtgg
    10441 gagaactttt acttatagat tatgatgttg tagaaccatc taatttaaat agacagcagt
    10501 attttataga tgatatagga aaactaaaaa ctgatgccat gatagaaaat ataaaaaaaa
    10561 taaatccatt tataaaatta aataaacgag atattttttt aaacaagaac aatatggata
    10621 ttattaaaga ttcagattta ataattgagg cctttgatga tgcttcatgt aaagcacagg
    10681 tatgtaatta tgtattaata aatttaaaag ataaatattt aatagcatca tctggtatgg
    10741 caggttacta tgattcaaat ataataacta ctaagaagat aaaagacagg ttttatatat
    10801 gtggggattt tgtgaatgaa gctaaatttg gtgaagggct tatggctcca agggttgcca
    10861 tctgcgcaaa tcatatggca aatttggcaa cgcaaatact tatagatatg taaatattaa
    10921 aattagatat aaaagagatt gaatattaaa aaaatttaac gtggagggaa ataatatgga
    10981 taaattggta cttggtggac atgaatttaa tagcagatta ttggttggaa caggtaaata
    11041 tggttcaaat aatatattgc ctgaggtaat aaaagaaagt ggaagtgaaa taataacaat
    11101 ggcacttaga agagtggatt tagacaataa acaagaaaac atattaacgt atattccaaa
    11161 agagatgaaa atacttccaa atacatcagg agctactaat gcagaagaag ctgtaagaat
    11221 agctagaata tctagaaaaa tgggatgtgg agattttata aaaatagaag taatatcaga
    11281 tactagatat ttacttcctg ataatgaaga aactataaaa gctacaaaga tacttgctga
    11341 tgaggggttt atagttcttc catatatgac accagacctt tatgctggaa gaagacttat
    11401 agaagctaat gcagcatcag ttatgccact tggagctcca attggttcaa atagaggtct
    11461 tcaaatgaaa gaaatgataa gaataatgat agatgagttg gatataccaa taatagtaga
    11521 tgcaggtatt ggaaaaccat ctcaagctat ggaggctatg gaaatgggag ctgatgcagt
    11581 acttgtcaac actgctatag cttcagcagg agaccctgta caaatggcta gagcatttaa
    11641 attagctgta gaaggtggaa gggaagcata tattgcaaaa acaggaaatg tgtcagaatt
    11701 tgcaaatgca tcttctcctt taacaggttt tttaggcaat ttatagaaag tggtgattgg
    11761 tgatgagttt ttatgatgta atagaaaaat atagggattt tgattttgat ggatatttaa
    11821 ataatgttac tgataatgat gttttaagaa gtttatcaaa agacaagctt gaagattttg
    11881 atatattaaa tttgctttct aaaactgcag taaaacattt agaagatatg gcacaaaaag
    11941 cacataaatt aagtgttcaa tactttggta aaacagtatg tttatataca ccaatgtata
    12001 tagcaaatta ttgtgttaat caatgtgttt attgtagcta taatataaag agtggaataa
    12061 aaagaaaaaa attaactatg gatgaaatta gagaagaggg agaagcaatt tcaaaagaag
    12121 gtttcaaaca tctacttgtt ttgactggag aaagtagttt tcactcaagt gtagaatata
    12181 taggtgaggc tatagaaata ttaagagaga aatttccctc tataggtata gaagtttatc
    12241 caatggaagt agaagaatat aagtacattg tagataaagg tgttgaagga ttaacagtat
    12301 atcaagaaac ttatgacgag gaaatatata agagagtaca tataaaaggc ccaaaatcaa
    12361 attataaatt tagactagat gctcctgaaa gaggggcaaa agcaggtatg aggactttat
    12421 caataggtgc acttttaggg ctaaatgatt ttagaaaaga aacttttttt acaatattgc
    12481 atgggaaata cttaaaaacg aaatatcctc atattgagct ttcatattca acaccaagaa
    12541 tgagaccttt taaagggtgt tttgaggaat tggtagatat aagtgatact gatttagttc
    12601 aagctatggt atgtatgaga ttatttgacc ctcatgcagc tataaatata tccactagag
    12661 aaactttaga aatgagaagt catataatac ctttaggagt aactaaattg agtgcaggtg
    12721 tatctactga tgtaggaggt cattctcaag atgaacatga tactgctcaa tttaagataa
    12781 atgatgaaag tactgttaaa gatgtggaga aaatgctaaa ctcaattggt tatcagcatg
    12841 tgtttaaaga ttgggaaaga ttttaaatgt atctgataac aaacagaaaa ttatgtagtg
    12901 aggaacgata tttagaagtt attaaagagt ctatattgag tggtgtagaa aatataataa
    12961 taagagaaaa ggatttggaa tatcaagagc taaaaaaatt atatatgaaa ataaaaacta
    13021 aaatcaattg tatagatttt caagaacaaa tatcagatga aagtttgaaa actaatataa
    13081 atcaaaaaga gtgtagaaat aaattcaagg ttaattttat aataaatagt aacattgaat
    13141 tttttgaaaa agtggattgt caaggtatcc atcttccttt taaattattc ttgaatttaa
    13201 ttgaaaataa gtataatttt aatgaaaata aaatactagg attatcttta cataaagtag
    13261 aggaagtaga ttatttagaa aaattaataa gaaatcaaaa tataaaaata gattacataa
    13321 cattatcaca tatatatgaa actaaatgta aagaaggatt aaatccaaaa ggaattgagc
    13381 ttttaaaaga agctaaaaag attactgata ttaaaataat tgcacttggg ggaattttac
    13441 catctaatgt caaggaaaca ttaaaatact gtgatgattt tgctataatg tcaactataa
    13501 tgaggtctaa agatattaaa aaaaccatat caaattataa tgaaaagttg aattagtaaa
    13561 tatagtatat tgaattttta acttaaataa aaataaaaaa gttatctttt aaattttaaa
    13621 aggtagcttt tttattttat atctttgcta ataaaaaatt gtaaaaaggg aaaactttag
    13681 tcagagagat taaacaatat cctagaaaga tttaaaattt agtaaagtat ggaggacatt
    13741 ataatgactg acagtaaaaa gaaacgattt attataccac acacttttac aataatattt
    13801 ttactaatag tcttgatggc aatattgact tggatagtac caagtggtga atttagtaga
    13861 gaagatgtag aaggtagaat ggtagttgta ccagggactt atcaaagtgt agaaagtaat
    13921 cctcaaggat ttgcagatgt ttttaaagca ccaatagaag gctttatcga ttcagctgaa
    13981 gttgtaggtt ttgttttaat agttggaggt gcttttggta ttgtaaatag gactggagct
    14041 atagaagcag gaatagcttc ggttatctct aagtttaagg gaaaagaact tcttataatt
    14101 ccaatatcaa tgattttatt tggacttgga ggtacaacat ttgggatggc agaagagaca
    14161 ttaccatttt atatgatatt tgttccactc atgacatcct tgggatatga ttctctaaca
    14221 gcaatagcaa ttgtatttat aggagcagca gcaggagccg ccgcttcaac aataaatcca
    14281 ttttcagttg gtattgcaca ggcactagct caacttgctc ctggttcagg agtaacttac
    14341 agagtagtta tttttatagc atatatacta ataagtatag catttgttat gtactatgct
    14401 aagaaggtaa aggaaaatcc tgaaagttca atagtatatg aaatagataa aaataataaa
    14461 tcaatactta atcaaacttc aacagaaata aaagaattta cttttaaaga agtatctgtt
    14521 cttgctatat ttggaatagg gatggcaata atgatttggg gtgttttaag tcaaggatgg
    14581 tacattccag aaatatcaat gtgttttgct gcaataggtg tgctttctgg attggttggt
    14641 agattatctc aaattgaaat ctcagaaagt tttatagatg gagcaaaaga tttagcatct
    14701 gcagctcttg ctatagcttt ggctcgtgga atcgtaataa ttgctcaaaa tggatttata
    14761 atagatacta tattaaattc cgcagcagcc tttctaggtg ggcttccaaa ggttatattt
    14821 gtatatttat catttttctt ggaagctgca ttggcatttt taattccatc atcttcaggt
    14881 cttgcttcac ttacagtacc tgtacttgct cctcttggtg atttggttaa tatatcggct
    14941 caagtgatag taaccgctta tcaatttggt gtgggtctta caaactttat aactccaact
    15001 tcaggagtac tgatgggagc acttgcagta gcccatattc cattttcaaa atgggtaaga
    15061 tttataatgc cgatcttgtt gattttgaca gctgtatctc tagtattttt aacaataggt
    15121 atttatatag ggttttaatt aagtgtagtt ttatttaaga tatttagtaa tggtataaaa
    15181 tgataatatg ttttcaataa attgccacta atttaattag tggtaatttg ttttttgtat
    15241 ttaaaaataa atgtatttac ttgtattttg aattaatatc tacttagaca ttattttaat
    15301 tgttatattt tcatttaatt aagcattgat ttatcatttt ttatatttca tataacaatg
    15361 caaaaaataa tttttatata gtaaatgaca aaaatttaca ataaatgtat attttcttac
    15421 tcattcctta aaattactca tttagtttaa taactctcat ttcatatgta aagttggtaa
    15481 aaaattaaaa ttaaaagtaa aaaactaaat aaattaatgt taatttgaaa tatatatgaa
    15541 ggtttttgga gaatacttac ttatgatagg aaaaaatgtt aaaataatag tgtaatcaaa
    15601 tataaaggac tgaaataatt aacagttatt aaatattagg agtgtaaaaa tttatgggat
    15661 taaaagaatt tgattttatt gaagagtcta ttgatatgtt gaggacaatg tcaccaacat
    15721 tagaaactat atcagatgaa atagaagagt attttgaaaa tatcttggat gaaaagaatc
    15781 aagaatatat aaatgtaacc tctagaataa aatcagaatc aagtttaaga gaaaagataa
    15841 ttagaaatag atatttaaaa aaatatggag aagcaagtaa tcttattcat aatgtttctg
    15901 acttaatagg cctaagaatt gagtgtagat ttattgagga tgaaaacaaa atatatagac
    15961 ttttgagaag atattttaat aaaacagatg acaaaataga ctattataat aaagagaata
    16021 agaatattaa attaaaacta tctgagagac aacccaataa gcaaaaaaat ggatttgaaa
    16081 tatataaaat agatggagtt tttgcgtatt tagatagaga agtaaaattt gaacttcaaa
    16141 ttaaatctct tgtaaatgtg ttttggagtg aaatagaaca taaaataatt tataaaaaca
    16201 atacatattt acttgctgat aaatttataa aggatatgat ggactctata aagaataatc
    16261 ttactatgat agataatcag cttttaagta tttataaaaa ctttcattca ggaaagtctt
    16321 ttaatatgaa agtaagtaaa aaagaaattg aaaaattatt tgcgaaatta gtctatgacg
    16381 ctttttctga aaagatgaat aaaagtatag gttttgttgt agattttaaa aaaccatgtg
    16441 agactatatt gaactattct tttaataaac aagatataag tgatgaagtt ttaggtaatt
    16501 ttatgcttga tgagtttgca agattaaatg aaattgtaaa caaggatata gattttaatg
    16561 agcaaataga atttgaaaga gaaccaacat ttgatgataa gttttgtaaa gacctaggaa
    16621 atcattttag aagtagatta aacacagaat ttccatggaa cctatttttt agaatattat
    16681 ttgaaataga gccatataac aataccaaag actttgaaaa ctttgtagag tttataaaag
    16741 aaaatgtgct actagaagaa aatagggagc aattattatg ccaatttgaa gaagatagca
    16801 agcttataat tgaagatata tatgattgta tatttaatag tataagtgag attgatagtg
    16861 ttgaaatttt atataactat aatcttgaaa aaataaactt tacatcaagt gaaatcataa
    16921 attgcatatg tagagaatat gagtgttatg gtgaatactt agaagaaaaa gaaaagctta
    16981 tgaatacatt taaagaaaag attattcaaa tctttgagta ataggtcaat gctgaagtgg
    17041 cgctaatgcg ccatttttca aatatatatg aattaagtaa aattattttg gaggaggcga
    17101 tttgtataga gaaaacaggt gtaattttgg cagggggaaa aaattctaga atgggtagag
    17161 ataaagcctt tttagagtta catgataagc tttttattga aattgcaata gaagcattta
    17221 aaaactttga tgagttgata ataatatcca ataatgaaga attatattct aaatacgata
    17281 ttaaagttta taatgatata gtaaaagatg ttggtccaat tggaggaata tatactgctc
    17341 ttgaatatgc aaagtatgat atagttacaa ttgcatgtga tatgccatat ctaaataatc
    17401 aaatagtcga aagaatagct aataaaatga atgataaaag tgtgatttca gtgacaaatg
    17461 gaaagttaca accattgtgt tctggatata aaaagagtat aataaataaa gtatctttat
    17521 gtataaggga aaatgattta aagctcagaa gctttataga taaaatagat aaaagttata
    17581 tatactttga tgaagaagat ttatttttaa atgtaaacac tgttgatgag tataaaaaat
    17641 taactaaagt ttaattagct atggttgtaa aatagagtag gatttatatg gaggagagtt
    17701 tatgaattta cttaaatgcg attcttgggc agtaatatta aataatagcg ataaagaaag
    17761 taaagcatat aaaatattag atgaacttaa aaggaatatg tacaaagttg ttgctataga
    17821 tgaagagaaa aaacctattg aaggaataga tgtgtatgaa tgtttaaaag atgttccaca
    17881 caatattgat gttgtagcta ttattgataa acaatctaag atggatgtga ttttagaaga
    17941 agtagagctt ttggatatac aaaatatgtg gtttgaaaaa ggaagcttta gtgaaatgat
    18001 gattaaaaag actaaagatt taaaattaaa tatagaatat aatttaagtt tatatgatga
    18061 gttgactaga taaaattata tatcttaata ataaaaatat aattagttaa taaaacatat
    18121 tattttatag tatagattgg ttaataaaag tacagattgg ttaatgaaac attaaattac
    18181 aatatttgtt aaaaaaattg tgagttacat gatataatgt attaaatagg atactaagga
    18241 gttgatatat tatgttaata gtaactacag aaaaagtaga aggtaaaaag atatcaaagg
    18301 ttttaggatt agtgagagga agtacaataa gagcaaaaca tgttggaaaa gatataggag
    18361 caagttttaa gaatcttgtt ggaggagaac ttactggata taatgaaatg ctcactgaag
    18421 caagacaaat tgccataggt agaatggttg aagatgcaga agctaaaggt gcaaacgcag
    18481 taatagcatt tagattgtct tcagcttcag ttatgcaagg ggcagcagaa atgcttgctt
    18541 atggaacagc agttgtttta gaagatgata atagtattct tgaaaaataa aatataaata
    18601 gttaaaaaga ggtatctttt aaaaatttta tggatgcctt tttttatttg aaaaaactaa
    18661 tttgagaaga aattttaact attatgacaa taaaattagg gtgatattat ataaataagt
    18721 aaaattatat tatataatgt atggtataga ctttaaaaat tttatttgta gagaaaaata
    18781 tggacaatgt tataataatt atagaaatta aaagtactta cattcattat gtatgtgaca
    18841 tgtataatta gttttatatt taatacagtt ttgaagtaat atcttcattt tgagaacgtt
    18901 ggaataataa aaaatgtatg tattataggt gatataaata aaaaataaat ataatatgtt
    18961 atattgctga tagaaaagag aataaaagaa aataggtttt attaaaagag atttttattt
    19021 ataggactta atcaatgtat agaggggtga gaacatatgt ttaatgttgc aattataaca
    19081 cttagtgata aaggatatga aggaaaaaga gaagatataa caggaaaaaa gttaactgaa
    19141 tttgttgaaa atacaggagc ttataaagtt actgaatatg tacttataaa agatgataag
    19201 gaaatgctaa aggaaaatat tataagactt tgtaatagta ataaaataga tttaattctt
    19261 acaaatggag gtactggttt ttcaaaaaga gatataaccc cagaagcaac taaggaagtg
    19321 ttggaaaaag aaataccagg tctgagcgaa tatatgagaa tgaaatccac agaaattact
    19381 aaaaaggcca tactaagtag gggtgtaagt ggaataagaa ataattctat aataataaat
    19441 ttacctggaa gtcctaaagg tgcagttgaa aatctaagtt ttataattga tgttttagac
    19501 catggcatag aagttttaag aggagaagct acagaatgtg ccactaaaaa aagtgaatag
    19561 tatataatct agatagataa aaaatagaca aagaggtaga taaagtatga ttgataagta
    19621 tggaagaaaa atagactacc ttcgtatttc acttacagat aagtgtaatt taaggtgtgt
    19681 ctattgtatg ccaccagatg taaaatttga taaaaattat ataaatgaaa atctaagctt
    19741 tgaagactac aagtttatta taaaggctat ggctgaacaa ggtataacaa aaattagatt
    19801 tactggagga gaacctcttt tatatcctaa attaaatgag cttataaaat ttacaaaaga
    19861 agaatgtgga ataaataaca taggaatgac cactaatgct attgggcttt ctgatagaat
    19921 ttttgagctt gaaaaatgtg gcttaaataa agttaatata agtttagatt ctttgaagga
    19981 atataagtat aaatccgtaa caagaggagg aaacttaaaa gatgtcttaa aatcgattga
    20041 atcttgttta aatttaggta taaaagtaaa gttaaattgc gttgctataa gtgggtttaa
    20101 tgatgatgag cttaaagatt ttatgttttt aacagttaaa tcaccaattg atgtaagatt
    20161 tatagagctt atgccattag gtgaggctaa tagagtttat aagaagggtt attttaatgt
    20221 aaaagaaatg attgaaaaaa tatatggttt atataaagtc aaaaatgatg aaaaaagtac
    20281 agcagaatat tatatgttta aaggagctaa aggaagaata ggaattataa ctccattaag
    20341 ctgttcattt tgtaacactt gtaatcgtat tagacttact tctagtggtg caataaaatt
    20401 atgtctacat tcaaaagaag aaatagatat aaaaccattt ttacataaac cattgttatt
    20461 tagagaatct atgaaagata taataaaaca aaaaccagaa aaacatcatt taatagaaaa
    20521 taaatgtagt gatacaaata ggcgtatgta tgaaattgga ggataagtaa tgttaacaca
    20581 tataaatgaa aaagggtatg ccaagatggt ggacataagt gaaaaagatg atactaaaag
    20641 aacggccgta gctacagcta caattactct tagtaaggaa gcattagaaa aagtaaaaaa
    20701 tggtcagata aaaaaaggag acgtattgtc tgtagctcaa gttgcaggta taatgggagc
    20761 taaaaataca tcttcaaaca taccaatgtg ccatcagatt aaattagttg gatgtgattt
    20821 agaatttgat gtagatgatt ctatgtctgc tattaagata tacgcaacct gtaaatctct
    20881 tgggaaaact ggagtagaaa tggaagcttt aaatgcatgt tctttagcag cacttacaat
    20941 ttatgatatg tgtaaagcag ttgataaaaa aatggttata agtaatatcc atcttgtgga
    21001 gaagactggt ggaaaatcag gagattttaa gttcgagtaa attaataata tattgggtta
    21061 aaatttggag gaaatatggc gaaggtagtg tcaataaata taagtgagaa aaagggtgta
    21121 attaaagttc ctgtacaatg tgcagagcta aaaataaatc atgggatagt tggagatgca
    21181 catgcaggaa actggcatcg tcagataagc ttacttggaa aagaaagcat agataaaatg
    21241 aaaatgaaag gctttgacca attaaaatat ggagattttg ctgaaaatgt aactacagaa
    21301 gggatagaag ttttttccct gcctgttggt acaaagttaa gaatagggca atgtgaagtt
    21361 gaagtaactc aaatagggaa aaaatgtcac aatggatgtg aaattaagaa attgacaggt
    21421 gactgtgtga tgccacgaga aggaattttt gtaaaggttc taaaagaagg aattataaat
    21481 gtgaatgatt caatagatgt aatctaatga agtcactatt aaaatataat tataagtaaa
    21541 tactattaag atttaaatat atattttaag agagaattaa ttttaataaa atattttagt
    21601 tataaattgg aatattggtt atataaagct atgaatgtaa aatcatagct ttttgttttt
    21661 gaattttaaa atataatatt ttatttatat atattaaatt atattttata taaaatcaat
    21721 tgtttaaata tatattattt atactgtaaa tttatagata ataaagtata aaaaaaatat
    21781 attttgatga aatttaaaaa atacattata taaatatagt aatagtatga aaatttacat
    21841 ataaaactaa aaaatgcaag taattgactt tttaagttga caaaatattg catatatatt
    21901 atgatataaa tcataaatta actacaaatc aatattatat attcgtagaa gaagaaagta
    21961 gcataaagga attttacaga gagttagatt tggctgagaa ctaatattgg aatttatgtg
    22021 aaaaggactt tggagaatac tattgtaatt atgttagaat agtggtttaa ttcgccttaa
    22081 gaaaatgagt gagtaaaatt aatttttact aataagagtg gtaacacgga tttatattcg
    22141 tctcttggat ttttccaaga ggcgtttttt attataaact tatttcagaa aatttaaaac
    22201 tatatattaa atgaaaatta taaaaatatg atagttaata aatttataaa ttagaaaatt
    22261 gtaaaatatg ctaaggtcag tagttttacg atttaaatgg agggatgaaa atggaagaag
    22321 aaaaaattaa ctatgctctg gaacagattc cagagaatca aaaacgtggt tgggtagcta
    22381 tgttttcagt tttagtagca ataggtgtgg atttatcatc tgttatttta ggcgctgagt
    22441 tggcacagag tatgccaatg aaacaagcaa tattgtcagt aattgttggt tcattttttt
    22501 ctgcaatttt gtatactaca tgctctttgg ttggttcatc tacaagttta tctacatcta
    22561 tgataacaaa gtatgtattt ggagaagctg gagcaaaaat attttctcta gttatagggg
    22621 tttctctatt aggttggttt ggagttcaag taggtttctt tgcacaaaat gcacagataa
    22681 taataaaaga tatatttaat ttagatgtca gtatgcagat tttaagccta ataggtggtc
    22741 tcttgatgat gagtactgca atttatggat ataaggcaat ggagaagttg agtgtgtatt
    22801 cagtaccatt tttattagtt cttatgatgc taactatatt cttagctttt agagcaaatg
    22861 gaatttcagt tgatgataat atgaaatcta ctatgacatt tgcaggtgga gtttctctgt
    22921 caatgagtat aataatagtt ggtgcaattg tatctcctga tataagtcgt tgggctaaaa
    22981 gtagaagaga ctgtgctctt tcaagctttt tgggaataca atttggaaat gcttttatga
    23041 taatagtttc gatagtatta gtaaaatgta tgggaacttc agatattatg agaattttta
    23101 ttacattagg aattgctata ccaggtataa tagtacttac attagcacaa tggactacaa
    23161 atacaagtaa tgtatattca gcatcattaa gtatagctct tgtacttaag aaggctcctg
    23221 agaaagtatt aactatagtc cttggtatta tagcaacatt attggctgta tttggaatat
    23281 atgaaggttt tataggattt ttaaatttac ttggaattgt aattgcacct gttggtggtg
    23341 tatatacagc agaatattac atagtaaagc aagaacttaa aggctttgat aaaggagtat
    23401 tatataaacc aatagttaaa cgttcaatag tgtcttggat aattggtata ttaataactt
    23461 acctttcaac atatggattt ataactctta ctactatagc acctttagat ggttttatag
    23521 cagggtttgt agtacaatct attattggaa aagtgctttg ttcaacaaaa aataagcaag
    23581 aaatagacaa agcagtataa agaaatttgt ttataaagta taaaataaat tagaaatatg
    23641 aggagaaagt aacatgagat ttttaacaga agaaagtgta gataaaatag cagttggtgc
    23701 agcagtgctt ggaactggag gtggaggtga cccatatgta ggtaaattgg ttgcaaaaca
    23761 agccattaaa aagtatggtc ctgtaaaagt aataagttta gatgaacttg atgatgatgc
    23821 tttggtagtt ccagtatctg gtatgggttc tcctgtaatt acaatagaaa aattactttc
    23881 agaagtagag ttaactaccc ctttagaaat aatggagaaa ttattaaata aaaaagtgga
    23941 tgttataatt cctatagaaa taggagggat aaactcactt atgcctatag cagttgctgc
    24001 taaaaaagga cttcctatct tagatgcaga ttctatgggt agagctttcc ctgaagctca
    24061 aatggtaact ttttatcttg aaggatatga ggcatctcct gccgtaatgt ctgatgaaaa
    24121 aggtaattct gccatccttt atcctgtgga tggaatttgg tcagaaagat tagctagaac
    24181 tttaactgta gaaatgggag gaagttcttc tattagtgac tataatttaa gtggagctca
    24241 agttaaaaaa gctgctatag gtaatactct tacaatagct gaaactatag gagggttttt
    24301 acttgaaaat aaatctgatt caaaaggtgc tattgagaat atactaaaag aattgcgtgg
    24361 atacaagtta tttgaaggaa aagtaattga tattaagaga gagttgaaag gtggatttac
    24421 tagaggacat gccttttttg ctgggattaa tgagtatgat ggagagtatt ctatattatt
    24481 ccaaaatgag aatttaattg ccaaaaaagg tgatacacca ctttgtatta caccagattt
    24541 aatagcggta ttagatttag aaactggatt tccaataact acagagagaa taaaatatgg
    24601 ctctcgtgtt atggtagtag cttttccttg taatgaaaag tggagaacag aaaaaggaat
    24661 tgagacagtt ggacctggat actttggata tgatgtggaa tataaaacag tagaagaatt
    24721 acaaggaaaa taaaagttta ggaggaaatt ttgatgtatc gtgtaggaat tgacgttgga
    24781 ggaacaaata cagatgcttg tatactggat ggggaattaa aagtaattca ttcagttaag
    24841 gtggcaacta caaaagatgt tgaaactggt gtttacaatg ccttaaaaaa agttatagat
    24901 gaaagtaaga ttgaccattc attaataaaa tatgctatgc ttggtacaac tcattgtaca
    24961 aatgctattg tagaaagaaa aagacttaat aatgtggcta tgataagaat aggaaagcca
    25021 gccactactg ccattcttcc atttgtagat tggccagaag acttaagaga aaaaatagaa
    25081 ttagattcaa tattggtatc tggtggatat gagtttgatg gtagaaagat aaatgaactt
    25141 tgtaaagatg aagttgtaga gttttgcaat agaataaaag gcaaagtgga aagtgttgca
    25201 ataagtggag tatttgctcc agtaaataaa caacaagaag aagaagttgc aaaatgggtt
    25261 agagaaattt taggggatat tcctatatct ttatcaaatg aaataggtaa tataggtata
    25321 ttagaaagag aaaatggagc tattttaaat tctgcattgt ctcaagtagg taaagctgta
    25381 tctttaggat ttgaaaaagc acttaaagac ttaggtatag atgctcaaat atatttcagt
    25441 caaaatgatg gaacattaat gaatttagag tatacaatga aatacccaat ttttacaatt
    25501 ggatgtggaa taacaaattc tataagagga gcttccttct tatcaaaagt aaaaaatgca
    25561 atagtattag atgttggtgg aactacaagc gacttgggtg tactatataa tggattccct
    25621 agagaatcat ctattccagt tacaattgga ggagtacata caaactttag aatgccagat
    25681 gttttaacta ttggtcttgc tggtggtaca tgtgtaaaag gcgaaaaaga taatataaag
    25741 ataggtcctg aaagtgtggg atatagaata actgaagatg caataatatt tggtggagac
    25801 actctaactt tatcagatgt tgttacaaaa ttagatatgg cgtttgaaga tagaaaaagc
    25861 aatgttgaaa acttagataa agagtattgt aatgaggtat ataagaaggt tattttaaaa
    25921 ttagaagatg ctgtagacca aatgaagact agtcaagatg atgttgaatt ggttttaact
    25981 ggtggaggaa gtataattgt tcctgaaaaa ctaaaaggca taagcaatgt tataagacct
    26041 cctcattata ctgctgcaaa tgctattggt gcagctttag gacaaataag tggagatgta
    26101 gaaaaagtat acagtttaga taaaatgtcg agagaagatg ccatagaaga tgcaaaaaaa
    26161 gaggctgtaa ataaagctct aaaagctggt gctaaagaag atactataga agtattaaat
    26221 atagaagatg ttccacttgc ataccttcct ggtaatgcac ttcttataaa agtaaaagtt
    26281 gttggtgatt taatttaaag aataaatata agtatttcca gaattatata aatatttctg
    26341 gactcatttt taaacttatt ttatcaataa aaacagcata acttttgact atattcttat
    26401 aagtttccga acagccgata tatacctatt ttttgaggtt tatcggctgt ttgttatctt
    26461 ttatcgcatt ccaactattt ttcttaccgt atttgctgta cgaattgtaa tcttatcact
    26521 tacatttgaa ctggcagtct ttgaccacca atttgtcttt ttataatctt tacgactaaa
    26581 agaccaaaag atagcatttt tataatgata tactttttca tactcttctt taggatttcc
    26641 aacctcatca taaaactcgt cataagataa gtcaggaaac ataaaaataa gattatcata
    26701 tatttctttg ttgttatctc cccaccaatt aggagcatta tttagtatat cttttaactc
    26761 ctcttgtgaa ataataaaaa ctggtatttc caattcaaac tgttttttta tcattaatct
    26821 tattgtattt gagataacct tgctgtcatc tacatcactt aaaaagacaa cattaccact
    26881 attaaggtat gtagaaactt ctgaataacc aagttctaca aagcttgctt ttaattcaga
    26941 cataattatc ttgttcttac cacttatatt gatacctctt aataatgcga tataactttt
    27001 catataataa tttcacctct aaacattcac atttttctat gtagtacaaa cttgaatttg
    27061 catcttttat aattctttca aaacttcttc atatgtaatt tttttaatat aaaaaaatcg
    27121 gtgcttaatt tgtaccgatt tttgtttttc ttagtaaaga actcgttttt ttgcttctgc
    27181 tatagctcta gcagcacttt ttgcttgcat tcttttttct tcttcttttt taaattcatt
    27241 tattgtttca tttagattct tatcatgttc atttttttct ttatcttcgt tttctctgta
    27301 tactaatata taatctatta ttgattcgtt tattatttga gatatacttt tattttcatt
    27361 aattcctatt ctcttaactt ccttaagtag agtctcatca atatgaaccg ttgtttgctt
    27421 tgtatttttt gccaccttaa tcacctctta aaaaaaattc taacattaaa atttttttta
    27481 gtcaataaat tttaatatta aaattcttta aatgttttat ttgattttct aaaattttct
    27541 attgtattta tcatcttatt ttttatattt tcacctagta cagaagactt tagtctagga
    27601 ttttcgatat ggtcataaat tggttctaaa aattcgactt cgacaggaac agagttaatt
    27661 ttaccaatag gttggtatcc ttcataagta tcttttgaat tttttattac tagtggaaca
    27721 atagggcatt ttgctttata tgctatcttt aaagctccac ttctaaactc cgaaaccaat
    27781 gaatttggtt ctttaatcca tgttaaatct ccttctggaa acactgccat actttgacct
    27841 gtaagtatat tttgagaggc ttgggctata ctctttatac cttcacgatt gttttttctg
    27901 tttacatata cacatcttag taattttgcc cattctttaa atataggtat atttctccaa
    27961 actggttcat ctgcaattac acatccaatt ggtctttcta cacttgctgt tagtatgaag
    28021 ctgtcaagca tactagaatg atttacaaca aatagaagtg gttcttttgg cagtgtttcc
    28081 ttaccaatta tattaagctc tatgttcact atatctaaag attttttagc ttgtttttgc
    28141 ataaattcaa atttttcctt taagctaaat ttatcaggat tctttttaat tttaataagc
    28201 ctaggtatag aagatgtaaa acctataagt tgatatgtag caaattttaa gtaattattc
    28261 ataaaattat ctccttaaaa taaaattatt tattactgta aagattttaa cataaaataa
    28321 aatttaagtc tattttaatt caattttgta acatatattt taaatagaca gagatttaga
    28381 ttttatattt ccaattttag taatcagatt ttatcaataa attactattc tattaattta
    28441 attaaataac ctttaaattt aattataaaa actgttgcag aaatttctac aacagtttaa
    28501 aaaatttacc ttacaaaaac ttattttata acaagagagt atattataag gctagtacag
    28561 cccatttttt gcactgagaa attcttttgc catcatcacc ttgttctttg tcatatgcaa
    28621 attgattaag catttcacat ggaaatgtat cacatagacc acaatgtgta aggtttttat
    28681 cttcacaaca agattttaca ggacattgtt caccccaaaa tggtttctca atctctgtac
    28741 aacctttaca attgactttt tccttgtatc cacattcata acataaaatt ccacatcttg
    28801 attctatcat tttaagtcac tccttaaata taaattttat atgattgtat atcaaaattt
    28861 atacttttca ttgtaaaaat cggacaaatt ttttatatag gttgttggat taacaccaca
    28921 aatagattta aaatcattta taaaatgaga ttggtcgtaa taaccaatat cttgtgctaa
    28981 agttgcaaat ggtattttat tattttgtat ttcttgtaat acaaggttta tacgaagtaa
    29041 tctaaggtat gatttaacac tcataccaag agaattatta aaaattctat ttaaatgtcg
    29101 ttcactataa caactaattt gtgaaatgtt tttaacagac ataatagagt tctgttttct
    29161 agcattttca agaattggta tagtcatatc tactatattt gatttcaata agtatgataa
    29221 gaataacata tctaattgtt ctactaattc ctttatagta cttgttcttt caaaaatgga
    29281 attaacctct aagctaaata atgtgtaaaa tcttcaagtg aaatttttaa atctgtagat
    29341 tctctttgag aaagacctgt caaataatat actccacctg ggcgaaactc aacgaatact
    29401 ctaaaaagta cattttctat atcatttttt acaatagtag tttttgttgt ggctccccag
    29461 aatgcgcttt ctataccttt tttgtcaaat ttaaatatca tacatccact agcatcaggt
    29521 attaatgtaa gattttcttt aatacttgta tctggaatag atattgtata gtgtgctata
    29581 tactgtctta acaatttatg tggcaaaata tatgtatact gtttgcaggt atttaaaatg
    29641 tt
//
LOCUS       DAEMHW010000032        28967 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEMHW010000032 DAEMHW010000000
VERSION     DAEMHW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.032"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..308
                     /locus_tag="KPK67_001212"
     CDS             <1..308
                     /locus_tag="KPK67_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2458206.1"
                     /translation="KNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSR
                     AFQLRVTDKGEEVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            310..1404
                     /locus_tag="KPK67_001213"
     CDS             310..1404
                     /locus_tag="KPK67_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBG2458207.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIILNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1492..2022
                     /locus_tag="KPK67_001214"
     CDS             1492..2022
                     /locus_tag="KPK67_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2458208.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYGIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            2350..3183
                     /locus_tag="KPK67_001215"
     CDS             2350..3183
                     /locus_tag="KPK67_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2458209.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     EDIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDILVFIALLIIGMYKLFKKY
                     N"
     gene            complement(3434..4810)
                     /locus_tag="KPK67_001216"
     CDS             complement(3434..4810)
                     /locus_tag="KPK67_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458210.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            5078..6250
                     /locus_tag="KPK67_001217"
     CDS             5078..6250
                     /locus_tag="KPK67_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2458211.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNINKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKEGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            6264..6911
                     /locus_tag="KPK67_001218"
     CDS             6264..6911
                     /locus_tag="KPK67_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458212.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            6913..7422
                     /locus_tag="KPK67_001219"
     CDS             6913..7422
                     /locus_tag="KPK67_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG2458213.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(7786..8898)
                     /locus_tag="KPK67_001220"
     CDS             complement(7786..8898)
                     /locus_tag="KPK67_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG2458214.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            9196..9462
                     /locus_tag="KPK67_001221"
                     /pseudo
     CDS             9196..9462
                     /locus_tag="KPK67_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
     gene            9707..11434
                     /locus_tag="KPK67_001222"
     CDS             9707..11434
                     /locus_tag="KPK67_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2458215.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGGVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            11434..13224
                     /locus_tag="KPK67_001223"
     CDS             11434..13224
                     /locus_tag="KPK67_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2458216.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFFFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(13386..13694)
                     /locus_tag="KPK67_001224"
     CDS             complement(13386..13694)
                     /locus_tag="KPK67_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458217.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(13956..14504)
                     /locus_tag="KPK67_001225"
     CDS             complement(13956..14504)
                     /locus_tag="KPK67_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458218.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(14607..15278)
                     /locus_tag="KPK67_001226"
     CDS             complement(14607..15278)
                     /locus_tag="KPK67_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2458219.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEP
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYKTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            15690..16580
                     /gene="garR"
                     /locus_tag="KPK67_001227"
     CDS             15690..16580
                     /gene="garR"
                     /locus_tag="KPK67_001227"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBG2458220.1"
                     /translation="MKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDELISCGA
                     KYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTPTESIL
                     CANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSSSILIG
                     DNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTILDAKI
                     PMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGHLEDDH
                     SGIAQYFEKLAGVEIKKHNN"
     gene            16716..18098
                     /locus_tag="KPK67_001228"
     CDS             16716..18098
                     /locus_tag="KPK67_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG2458221.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            18170..19606
                     /locus_tag="KPK67_001229"
     CDS             18170..19606
                     /locus_tag="KPK67_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBG2458222.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPVETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEVLVPAGMTEFAKDKSFEYKSSDIGEWCEEKTKGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKILGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(19792..20646)
                     /locus_tag="KPK67_001230"
     CDS             complement(19792..20646)
                     /locus_tag="KPK67_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458223.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(20914..21237)
                     /locus_tag="KPK67_001231"
     CDS             complement(20914..21237)
                     /locus_tag="KPK67_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG2458224.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            21440..22069
                     /locus_tag="KPK67_001232"
     CDS             21440..22069
                     /locus_tag="KPK67_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458225.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            22381..22890
                     /gene="ruvC"
                     /locus_tag="KPK67_001233"
     CDS             22381..22890
                     /gene="ruvC"
                     /locus_tag="KPK67_001233"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBG2458226.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKAIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            22883..23485
                     /gene="ruvA"
                     /locus_tag="KPK67_001234"
     CDS             22883..23485
                     /gene="ruvA"
                     /locus_tag="KPK67_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBG2458227.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            23592..24611
                     /gene="ruvB"
                     /locus_tag="KPK67_001235"
     CDS             23592..24611
                     /gene="ruvB"
                     /locus_tag="KPK67_001235"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBG2458228.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            24929..25954
                     /gene="queA"
                     /locus_tag="KPK67_001236"
     CDS             24929..25954
                     /gene="queA"
                     /locus_tag="KPK67_001236"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBG2458229.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            25966..26451
                     /locus_tag="KPK67_001237"
     CDS             25966..26451
                     /locus_tag="KPK67_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458230.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            26530..27651
                     /gene="tgt"
                     /locus_tag="KPK67_001238"
     CDS             26530..27651
                     /gene="tgt"
                     /locus_tag="KPK67_001238"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBG2458231.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            27785..28078
                     /gene="yajC"
                     /locus_tag="KPK67_001239"
     CDS             27785..28078
                     /gene="yajC"
                     /locus_tag="KPK67_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBG2458232.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            28136..28498
                     /locus_tag="KPK67_001240"
     CDS             28136..28498
                     /locus_tag="KPK67_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBG2458233.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            28564..28707
                     /gene="scfA"
                     /locus_tag="KPK67_001241"
     CDS             28564..28707
                     /gene="scfA"
                     /locus_tag="KPK67_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBG2458234.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
BASE COUNT        10975 a         3337 c         4936 g         9719 t
ORIGIN      
        1 ataaaaatag gaattgttct ttgaatgatg tttctacaga atttgaagtt gataaagctc
       61 atacaacaag aactatatct aggttagaac aagatggata catagaaaaa atccaaaacc
      121 ctaatgatag tagagctttt cagttgagag taacagataa gggtgaggaa gtattagggg
      181 atataaaaaa catattttca aagtgggata accacataaa aaaagagttc tcggatacag
      241 aatataaaga actggtaaag aatttacatg tggtaaagga tataaaaaca gctgtggagg
      301 aagaatagga tgtattgtaa tttattaaaa ccaattaaaa ttggaaattt ggaattaaaa
      361 aatagagtat cttttgcacc aacaagcatg ggtcttaaat tggaagaaaa aattaaaaag
      421 ttttctgata ttgctaagtc aggagtagct ctgattactc taggagatgt atctattaga
      481 cctagttttc ataaggtagc tataagccta tcggatgaag atggagtcat taaatacaag
      541 aaaattgtag atgaaataca caattctggt gcaaaggtat cagcacaact attttgttca
      601 gattatgatg ttaatttaat taaagatact atgaatatgg gaattacttc acatgatgag
      661 ataaaaaaaa taatgaatga cggagtaaaa gattacataa caaatatgcc caaagaagaa
      721 attaaaaata ttataaattt gtttaaagta acagcattga atgcaaaaaa agcaggtttt
      781 gacatgattc aaattcatgg tgataggctt gtaggaagtt tttcatcaag tatatttaat
      841 aatagaaatg atgaatatgg tggaacgtgt gataatagaa gtagatttgc aagcgaaata
      901 attagtagta tccgagatga agttaaggac attccaatag actataaatt tgcaataaga
      961 caagaaaatc ctcattatgg aaatgcagga gttttacttt cggaagttga gtattttgtt
     1021 aaaaaatttg aatccctagg tgtaaacagt tttcatgtaa cacttgcaaa tcattcaaaa
     1081 ttagaagata ctataccaac caataatcat ccttacttta aagatgaagg ctgtttttta
     1141 tatttagctg atgaagtaaa gaaacacaca aatcttccag tatgtggggt tggaaaattg
     1201 tctagcccag attttataga gagtattatt ttaaataata gagtagatat ggtttctatg
     1261 tcaagacaat tactagcaga tagtaattgg ttacagaaag taaaagatgg aagagtagat
     1321 gagattaaaa agtgctgtta ttgtaataag aagtgtgcag atgctttaca aacacgctct
     1381 cagtttggat gtatactaga ttagaatatc cttataaaaa taaataattt gttaaggtta
     1441 atatattttt ggtgatttag ataagtattt agataaagag gaggagacat catgaaaagt
     1501 ttagttttat attcaagttt aactggaaat actaaaaaga ttgcttatac tatatatgat
     1561 gaaattcaag aagaaaaaga cattaaagat gtaaatgaat tagttgatta tggtattgac
     1621 tatgaaaatt atgatatagt attcttaggc tattgggtgg acaagggaat ttgtgataaa
     1681 aattcaaaac aagtgcttga aaatattcac aataaaaaga tagcactatt tggaacaatg
     1741 ggagcaagta aaaaaggaag ttatggagct agtataatag aaaagataga atcaattatc
     1801 ccaaaagata atgaaatatt aggttcattc atatgtcaag gtaagatagc agaaggattg
     1861 aaagcaaaat ataaagagat gttaaaactt tctcctgaca acgaacatat tcgccaacaa
     1921 ttaaataacc atgaggaatc tcaaagtcat ccagatgaac aagaaatata tgaagccagt
     1981 atgtttgcaa aaaatatgat gattaaagct agtattgtgt agtagttaaa caataagttc
     2041 atataagata tagaataaat aatttagtaa aaaagtatag caaataattt gctatgcttt
     2101 ttttatgaaa tcaaaaaaat aataaaaata cttgactgta aaccttgttt atacattatt
     2161 ttacagaaaa taagatagtt ttaaatataa aaaaagatat attattgaaa ggagatatta
     2221 ttgtatgaaa acttataaaa gataattagt ttgattttaa gatatactaa atatacaatt
     2281 ttatagagtg aaaatattta agactatatt atagttgtaa attaatagaa tagagaggta
     2341 gtgtttaaaa tgacaattaa agaagttgaa gaaaaaacag gattgacacg ctctaatatt
     2401 cgtttttatg agaacgagaa tttaattaaa ccccttcgta atgagcgtaa tggctataga
     2461 gaatattcag atgaaaatgt agaggacatc aaaaaaattg catatttacg tacattagga
     2521 attactattg atgacatccg taatattata ttacagaaag tatctttagt tgaagttgta
     2581 gagaaacagg agaaagttct cgaaactaaa atatcagact tagaaaatgc aaaaactatg
     2641 tgtaataaaa tgttagaatc tgatgagtta agttatttga atttgaaagt agagcaatac
     2701 ataactgagt tgccagaata ctggaataaa aacaaacctg tttttaaact tgactctgta
     2761 agtttcttat atatatgggg agggaaaatt acctggggaa ttattactat tgcttgtttg
     2821 ttgattgcaa taacagtatt tccaaagttg ccacctcaaa ttcctatcca atggaataat
     2881 ggtgtagcta attatgtaga taaaaaattt atttatgcct accctgtcat ttgtgttgta
     2941 ttcagaatac ttttacgtcc atttatatgg agatggttac aaatacatgc tttttatagt
     3001 gatactattg cagattattt aacgaatttt atgtgttttg tagcgttgtc tatagaaata
     3061 ttctctatac tttttattta caatattgcc agacatgtta caataattat ttttatagat
     3121 atacttgtat ttattgcttt actgataata ggtatgtata aattatttaa aaaatacaat
     3181 taatttttaa aaattaagta ataagtgata gtgtaaaaag tagccactgc aaattatgga
     3241 ttgtagtggc tattttatta tttgtatatg aaattatttt gtttttctaa taagttacga
     3301 tatattatgg tttatttcac tgataaaagg tttaggtatt ttaaagtaaa aagttgtata
     3361 ttctaataaa atttaaacat tttgattctt taaaattaga gaaaatacat tgcttttatt
     3421 aaaaaattaa atattaagaa tttaatttct gcatcttatt tagaaaagat aagaaatcaa
     3481 ctttatatat ttttttgggc cttcctcttt gattatcgag ttttgtattt acaatagttg
     3541 caattcctgc tttatataag atactcaata ttctattagc acttctaaga gttatgttca
     3601 tatagtctgc taatatttct gctgacacat tatttgaatt tctatcatta atcatacaca
     3661 tgattttaga tacatttgta ttagatagag gtataaaaga agcaacttta ttggctattg
     3721 agctatcttc aatattatta gaatttttat ttgaatataa agggcctatt gtatcattag
     3781 tatcatttac tataaaagta cagtttccat tcaaagaagc agatttacca ttagcttgac
     3841 gagcattttg ctctgcatga atatttgtat ttccaatgcc ccaacctata tttaagctat
     3901 atttaatttc ttttaaatct tctgaaataa aaaaatctgt atagttattg gtcatattgt
     3961 ttaacatttc tttagtagtg tgtatctcta ttttcatatc attttttttg acctgaactc
     4021 catagaatcc atgtcttgat agagaattta atatagtgtt atatatttga ttttgtatct
     4081 catcattttt aaatatcaaa tcttcattta tgtactctga actatttact gtaactatac
     4141 aagttgcaga tttatttttg tctaagtttt gtaatgttat ttcattcacc aaagatttaa
     4201 atgtattcag gattgattct gtagatggaa ccatgtaaat acaatcatat ccaagcttat
     4261 taagttcaca actaatgatg taatttctag taaaagaaag gttaatctta ttatctcgca
     4321 ataagtctaa atgtatttcc aataatttat catttaattt tttaaagtca tatacaggtt
     4381 gagggatatt aaaatcttta attgtatatg gacatttatt agagtctatt aatgaatata
     4441 tatcataata attgttgtcc tctcttaaaa aatccataaa tgattttgta aagtcaatat
     4501 ctttttggct atttaataat ttgaataagt atttataaaa atctctttca tcaagcttta
     4561 aaaaatttaa tggaactttt atgtctttta tatttattat aagttcacta tatggaacta
     4621 taccactgca tacaaatcca tcaaaattat ctacttgctc atagtatata ttaggtaaat
     4681 tattaaaagg attttcccct ttaagctcaa taaatgtaat ttcacaagta tcatttaatt
     4741 ctttaagagc ttgtttaaac ttatctgaca taaaataaga tgttgtcaaa aaaccaattt
     4801 tatatttcat aaaacctcct ttaaaatttc gagagtattg taactaatca atcaatttat
     4861 aacctcattt tataatagta gataaaacgc gtcaatttat tttacaaaag gataattgaa
     4921 agaaaaatca gaaaatataa aaaagactga aaaatatgtt gactggcaag aataattgtg
     4981 ctattttgta attaaggact agtcttttaa gatttcctaa aataaactta ggttttagga
     5041 aagaaataca ttttatttga aaataaagga gaagattatg aatacatatt taaatgaaat
     5101 ttctaagttt gttgatgaga atagagaaga aatagtttca ttatggaaag agattgttaa
     5161 tatagaaagc tatacacatt gtaaagaaag cgtaaataaa ttggctaaga gattaaagtt
     5221 tgagtttgaa aaagaaggtt tagattgcga attagtagat gttggagata atggtagtac
     5281 attgataggt acacttggtt ctaatataaa taaaaagcct ataatttttt caggccacat
     5341 ggatactgtc tttgaaacag gtacatttgg agagaatcca tttaaaataa tagaaggaaa
     5401 agcatatggc ccaggtgttt tagatatgaa gggtggtata ataatttcct tatatgttat
     5461 taaagcatta aataaaatag gatacaaaga aagaccaata aaaatagtat tttctggaga
     5521 tgaagagata ggacataagg attcaacagg tgcggatgtt atattaagag aagcaaaaga
     5581 aggtctttgt gcttttaata tggaaactgg attagtagat aatagtttgt gtgtaggtag
     5641 aaaaggaaga ataggatgta atatacatgt aaaaggtgta gaaacacacg caggtaatga
     5701 ttttgaaggt ggaagaaatg ctatagaaga aatggctaat aagatactac gcatacaaaa
     5761 attgactaat ttagatgttg gaactacagt aagtgtaagt gtgattaagg gtggaagagt
     5821 ggaaaattct attccagaag attgtagtat aaaagtagac cttagatttg aaaaagtaga
     5881 agaaatggag aatgttaaag aacaagttag agagatatgt aaagaaactt acatagaagg
     5941 aacttctaca gaagttaatt ttataagtga aatgatgcca tttgaaacta cagaagatgt
     6001 tatgagattt catacatttg taaatgaagt gtcaaaagaa aatggatttg gagaattaaa
     6061 tgctaagaga cttggaggta gttcagatgc aagttattta actattgcaa acgttcctac
     6121 aatctgttca tttggtgtaa gaggggaatg gaaccacaca tcaagagaat atgcagtagt
     6181 agattctatg tttgagaggg tgaaattaat ttctacagtt attttaaact tagataaatt
     6241 tgaggtataa agggggattt tatatggatg taacagctgg agataataag gtaacatgga
     6301 aagggtattt atctttattt gtattgatag tattattttc aggagtgttt aaagatagtc
     6361 aaggcttcct taaagcatta gattttgcta atctgacagg ttcatttggt actattgcag
     6421 atggtgtaga ctttatgggt aaaggaggaa cgggagcaaa agatggtttc ttatttgctc
     6481 tgactttgat tccaactgtc tgttttgctg taggtcttat agatgtagtt gaatctatgg
     6541 gaggaatgaa agcggcatca aagatattta atccattgct tagacctctg cttggtatac
     6601 ctggaatagc aggaatagca tttgtatctt catttactag ttcagatgta gcctcaataa
     6661 tgacgaaaga attatttgaa agtggggaaa taacagatga tgaaagaact atatttgtag
     6721 catatcaata tgctggttca gctgttatac tgaacacaat aaatactcaa gctccacttt
     6781 taccaatagc attattggca ttaggtccaa taatccttat agagatattc tgtaaaatac
     6841 ttggtgctaa tctagttaga ttaatcatag gtgtaagaaa taaaaagaat aaggctaagg
     6901 aggctgtgta gtatgagtca acaaaatact ggaaatgcaa ttgtaaatag taaacctaag
     6961 aaaactgctg ttgagatatt tatgggggga gcaaaaaaag gtttttacat aggtgttgaa
     7021 cagatattac cagccatgat aatgggttat gtaatagtgc agtttttaca attaactggt
     7081 ttagttgata ttttaggaaa tatttttgga ccagtaatgg gtatttttgg actacctggg
     7141 gaatctgttg tagttttgat agcagcattc tttgctaagg cagcaggagc agctacagct
     7201 gcaaacttat ttgcacaagg tctaatcact gcaccacaag caaccatatt aattatgcca
     7261 tgtatgttaa tgggtactct agttggtcat tatgccagaa tagtgcttgt agcaaatgcc
     7321 agtactaagt atagaatgtt actgttggca gttcctatat ttgatgcaat agtagggatg
     7381 ttgatagtaa gagttttact cactgttatg ggattaatgt aaataacata aaattataac
     7441 ctaactataa tgatatataa gaattaatat atacaaggat taatgaatag taattgatat
     7501 atagtaataa actaagatag taattaatgt gtatattaat taaaaaaaag agagtatctc
     7561 aaagtgaact ttttagttca tgagatactc tttttgtatg aaatcaagta tattttcata
     7621 atttcaacaa tgaaagagag gcttatcttt attttgagac agcctcttct tttaaattta
     7681 tatttctaga atttatgttt tttatttata caacattaac attagttatt agcaataatt
     7741 ttaagaaaaa tatactttca tgttaatatc agaataaatg tgtttttaaa ggatgatttg
     7801 ttttgaattt tcacgaatat ctacttctat gactttttct attgtatctc ctctaaaacc
     7861 taataaatta cttgtcaagt ttgcagtgga acaagaatga ttaggtataa ttcttattct
     7921 atcaccaatt tttatatcag tttcaccatt tactttaatt tttccaactt cttctgaaag
     7981 tgaatcaata gtaagttctg gatgtccaca taccctacca taaccaacaa tactactatt
     8041 tccatgtgca cctttatcaa gtccaaggca cttacttcca caatcaatta aatagtatcc
     8101 actgctattt tttgagatta cagtgcacac aacagtaaga gcacacatat cttcagtagc
     8161 acaacctaaa cctatctgta tagcatcata gtatacatag tttccaggtc ttacagcagt
     8221 tatagtttta gaagcagctt cagattcaac tgttggagta ctaccagaag cgacaatttc
     8281 tatattaaat ccattatcag ttagagcttt gtatgctttc tccatagaag aatcttcttg
     8341 aacacaaaca tctggaacac cttctggaga actacaacca tacacttgac ctggatgcga
     8401 acttatacca acaatcttga tgttttctag cttagaaatt tcagtagcaa gttcaaccac
     8461 tttttctggt tcaaccccaa aacggtgtaa gccacaatct acaataataa gtagcttgca
     8521 ctctattttt gatttagaaa aagcttcact ataagcctta gcacattcta tattatctgt
     8581 agcacagatt aaatttattt ttgatgccat agtcacaatt ctatttatat ttacttctcc
     8641 catgtaagga taagccaaca taatatcttc aaaacctgct tttatcagag cttcaccttc
     8701 atcaagtgtt ccaactagaa aaccactagc acctgcattt ttttgaagtc tcgctatgta
     8761 agtactttta tgtgtcttaa gcataggcca aagttgttta ttattatttg tacaaatttg
     8821 ttgaatttta tctatattct tttctaactg gtctaaatct agcaaaaaat ttggagtagc
     8881 gacttctgaa attttcatgt tatttaccac ctttcaacaa aagtaaatta actataagta
     8941 ttataattat tatattagta atatcggctt tataatatta atagcttata attatattat
     9001 agaactagcg ttaacagaga gcaatattta tttgtaataa ttagaatttt caataagtta
     9061 gtgtagtttt aaattattta tataatatat ttttaatttt atacaaagtt tatttaaatt
     9121 agtttgagga tttattaata gggtattatt gaatgagtaa aaaagtgatg tttacataga
     9181 ataatcacac tataaattaa tttagaacct aaaaaattaa tttagcaaat tggaaaagag
     9241 aaggataaaa aagatatttg gatagttggt ggaggcgata tagtaaaatt atttatgaaa
     9301 gataacttaa ttgatgatta ttatatatat atattgccaa taatattggg gagagggatt
     9361 tcactattta agtatggtat agataagact aatttgaatt ttaagaaagc tagtaatatt
     9421 ggagaacttg taaaattgga atacagtaaa aaacaaaaat aaattacatg ccaatttgtt
     9481 acaatgttat gaaatgaagc atttaaaaga cttttggcta tatgaattga cattacttgt
     9541 caaatatgtt aacatataag cgacttgtcg gagggtgtat taaaaagaat atgctggaga
     9601 agagagtgcg ttaactcttt tctccttttt ttataaaatt aagcatttat aaatttagta
     9661 ttattacatg attacactat ctcaaaaatt aagaaaggag aaaaatatga aattaatttt
     9721 aagttattta aagaattata agttattaat agtattaaac atactggcta tatttagttt
     9781 tgcattagtc gagttgggga ttccaactat aatagctaaa ataattgaca atggtattgc
     9841 aaatcagaat atagcttaca taaaacaaat gggaatagtg attgttatta tttctattat
     9901 agggggtgta ggtagtatat tgttgggtta ttgttctgct aaggtatcaa caagtgtaac
     9961 cagagatata agaaatgaca tatttgagaa atcacaagaa ttttcacata ctgaatacaa
    10021 taaatttgga atttcttcaa tgattacaag aacaacgaat gatgcatttc aaattcaaca
    10081 atttgttaat atactactaa ggactgcctt attaactcca gttatgttca ttataagtat
    10141 aataatgaca attagaacaa gtgtggaact ttctatagta ttggctatat cagtaccttt
    10201 tataattatt ggagtagcaa taatagcaaa agtatctcag gcaatatctt caaaacaaca
    10261 aaaaggacta gataaattaa accttatatc gagagaaaat ttaactggaa taagagtaat
    10321 tagagctttt ggaaatgatg attatgaaac agagcgattt gaaaaaacaa atacatatta
    10381 tgctaatgtt tctaagaaac tatttaagct tatgtcaatt actcaaccag cattcttttt
    10441 actattaaat atagctgttt tggctgtatt ttggatttca agtgagaaga taaatattgg
    10501 agagcttcaa gttggacagt tggttgcatt tttggaatat cttttccatg ccatgttttc
    10561 aattatgctt ttttcaatgg tatttattat gtatccaaga gcagaggtat ctgcaaatag
    10621 aataaaagaa ctattaaatg aagaaccatt gataaaaaat cctgaaaatg gaattaagga
    10681 tactgaaaat aagggtatca tagaatttga taatgtaact tttacctatc cagatggaga
    10741 agcttctgta cttaaggata tttcatttac tgcaaaaaca ggtgaaacag ttgcttttat
    10801 aggaagtact ggtagtggga aaagtactct aattaacttg atacctagat tttatgatgt
    10861 aacagaaggt agcattaaga taaatggagt agatataaga gaatatgatt taaaagcttt
    10921 aagaaaaaag ataggattca tacctcaaaa atcactgctt tttactggta gcatagcaaa
    10981 taatattaga tttgggaaac ataaggcagg agagtcagaa ttagaatatt ccgcaaaagt
    11041 tgcacaagca tatgagttta tatcaaaaaa acctagaaag tttgatgaat tgattagtga
    11101 aggtggagca aatgtatcag gaggtcaaaa acaaagactt tctattgcaa gggctattat
    11161 aagaagacct gaaatataca tttttgatga cagtttttct gcacttgact ttaaaactga
    11221 tgcaatactt cgtgctaaat tgaagaagga aacaaaagat gcaattgtat tgattgttgc
    11281 ccagagaatt agttctatta ttgatgctga taagattata gtactcaatg aaggacaagt
    11341 tgtaggaatg ggaactcata aagaattatt aaaaaactgt gaaatatact atgaaattgc
    11401 aacatctcag ttaaaaaagg aggaattaga ataatgaaaa agtctaaagg ttttaagaaa
    11461 acaattggta gacttcttcc atttgtaaag aaatataaat ttttctttat tatagctatt
    11521 atatgtataa tttcagcagc tactatgaat gcacttgcac ctaaaactga aggattgata
    11581 ataacacaat tgactaaaga tgtaattagt atcgcaaaag gagtaccagg ggcttccgta
    11641 aattttgatt atgtaacaaa aatattggtg attttagcat gtatatattt tgccaatgca
    11701 atttttacgt atacttcaag ttttctactt acgaatgcta ttcaaaacac aatgagagat
    11761 ttaagaaatg aagtcgaaaa taaaataaga agattgccta ttagttattt tgatagtaat
    11821 agttttggag atgtattaag tcgtatatca aatgatgtag atacaatatc aaatgcattg
    11881 caacagagtt ttatgcaaat tgtaaactca atcttagtca taattttggc tctttcaatg
    11941 atgtttacaa ttaatatata tatggctctt atagctcttt ttataattcc aattagttat
    12001 tttgtatcta aatttgtagt aaaaaaatct caaagtagat tttcattgca acaaaatgct
    12061 cttggaaaat taaatggaaa agtacaagaa atgtatactg gatttaatga aataaaacta
    12121 tatggtaaag aagaagattc tataaaagaa tttaaaaaag taaatcaaga actttgtgaa
    12181 aatggattta aggcacaatt tatatcaagt atgatgaatc ctatggtttc attagtaact
    12241 tactttggaa ttgcagctgt ggcagttgtc ggttctatat atgcagtaag tggagggata
    12301 acagtaggaa atttacaagc atttgtgcgt tatatatggc agataaatca gccactttca
    12361 caaatgactc agttatctac agtaatacaa tcttcttttg cagctataga acgtgtattt
    12421 gaaattttag acgaagaaga ggaaatacct gatgttgaaa atccagttaa gatagaaaat
    12481 gtaaaaggaa atgttacatt tgagcatgtt aattttggat atggagaaaa ttcaacttta
    12541 atagaagatt taaatgcaga agtaaaaagt ggtcagatgg tagctattgt tggacctact
    12601 ggagcaggta aaacaacatt aataaactta ttgatgaggt tttatgatgt aaagaaaggt
    12661 gctattaaaa ttgatggagt agatataaga gatatgaaac gtaaagattt gcgttcaatg
    12721 tttggtatgg tgcttcaaga tacatggtta tttaatggaa ccatttttga aaatatagaa
    12781 tatggtagat ttggggctac taaagaagaa attatacaag cagcaaaagt agcaaatgtt
    12841 catcacttta taaccactct tccagatgga tacaatatgt ttttaaatga agaagcatct
    12901 aatatatctc taggggaaaa acagctttta accatagcta gagcttttat atcagaccct
    12961 tcaatattga tacttgatga agctacgagt tctgttgata ctaggctgga acttatgctt
    13021 caaaaagcaa tgagaaatct tatgaatggt agaactagtt ttgttatagc acatagactt
    13081 tctacaattc gcaatgctga cttgatttta gttatgaata atggaagtat tatagagcag
    13141 gggaatcatg atgaattaat ggaaaagggt ggattttatg agaagcttta taatagccaa
    13201 tttgcagaca aagaatcaga atagataaca taaacttttg tataaaattt tgtatcaaat
    13261 tcaaatatta atcatataat taaaaagagt atcagtaagt tgttttttta tagatttact
    13321 gatactcttt gattttaaga ctaagggtat ttatgattgg tacttataat tcacaactag
    13381 tcttgttaca tctgataacc agaattacag tacttttctt ttttaccttt ttgttctcta
    13441 acatataaca attcgcttaa cttatcaatg taataaccat catcacttat gcgaattaaa
    13501 ctagtcagat aatatccatc tttgcttcta ggaatctttt tatttattgc taaatcaata
    13561 gctgtctttg ctgcttcaaa atgctttaaa tgtgtatgac cttcttcaaa actctttcta
    13621 gtattataaa ctacataacc ttttttaact tgaaaaacta taaattcttt cttttcatat
    13681 attttattaa acattcaatc agcccttctc aagatagttt tatagcataa cattagaagc
    13741 ttctaaaaaa ttttacaaag aacataaata ttttataaat tataaatcta aaactataat
    13801 ttataaagat aactttctga ttatttatat gataattcat atagattaaa agtaaattat
    13861 ctttaggtta aacaaaagta aaataaaaga ctagttataa tatttatatg aaaaagtaca
    13921 aaatatagta tatgaatata gaaaatttaa attatttatt tacagttttt atttccataa
    13981 gtatatttac cttcagatac cttgtcatca tggaaatata ttctttcaca acttttatct
    14041 ccccatagaa tgttttttgt tattttatgg gacgtatctc ctgtaaatac ttcagctaca
    14101 gtagtatctg taacatcaca gtaaagagaa gcgaattttt taaaccaagg attttcctca
    14161 aatcttaaaa gccaggaagc tccatagggg caatgtaact cttcaaattc aggaacccat
    14221 cctagaaatg gaacatcagt tgtgctaata atattgtctg caattaattc atctccattt
    14281 tcatgagctc tttctctcat agcaatagct ctttcttcag acctttcaaa taaaactttt
    14341 cttaatattt cgctagtttt atcttcacca aactcttcaa ataacacttt ggaaaaataa
    14401 aaatataaat caccaaaatg tttgcatgcc atacgaactt cttcaatagc ataatctttg
    14461 tcaatagttt cattagttac aacatcttcg ttcaaaaaaa tcactcctta gaataagtat
    14521 tagttacaat ataattatag aatatttttt ataaattagg aaattaattt gagatagata
    14581 tatttttaat aaatgaaagt ataatgctaa aataacttat tgttacaaaa atcttctaaa
    14641 ttaatgtatt taggagtttc catatgaatg agagtagttt tgtaaatatc ttcaatacat
    14701 ccatttgcaa tattattaaa aatatctgtg tgctctttta atgttgtttc aagccttcct
    14761 tcatgtgaca atgaatcgag agctaatttt tttattctgt acatataatt gttaaacatt
    14821 ttgtcaaatt cagtattttg agcatatgaa actatagttg tgtgaaataa tgtatcgtat
    14881 tctgcaaatg agtttaaatt tttatcacct aataaaatat ctttttgttt atctaaaaga
    14941 tgttttaatt tacttatagt ttctactgct ctaggttctt tgtattgact tgtaattaaa
    15001 aaagtacaaa acccttctat tgcagagcgt atttggaata tctctacaat ttcatttgct
    15061 gtaatttgat ggattctaaa tcctttgctt gggattatat ctataaatcc atcttgacta
    15121 agtctttgaa gagcatctct tataggtgtt ctagagatac ctatttcact agcaagtttt
    15181 gtttcagaat atatttcatt ttctatcatt tctccagata gtatcaattc ttttaagtag
    15241 tcatatgctt gttcttgtaa tcttttaaat gtagccatac ttaataatac ccccctaaac
    15301 aatataaaaa tacaaaaact tatataattt taagcataca acatagtgaa aataaggtca
    15361 atcttttgca atttaaattg cagataagat taaaaaatat aaaaaattat tgaagtgtga
    15421 agcatgatta acattaaagg cagattaatt aaaactaatt gaaattagtt aaaatacaaa
    15481 actgtaaaag tagtatatta tatttgaaaa caatagtatg tattaaaact tatataggat
    15541 gactaaatat aataaatgac tttaaaaatt actatttatt gttaaaataa atacttttat
    15601 attttatatt gaccggtata ctggtatgta gtatactcta aaacataaaa aaacgttttc
    15661 aaaaatgtga agatatggag ggttataaaa tgaaattggg atttattgga ttaggaataa
    15721 tgggaaagcc aatggcaaaa aatttattaa aagatggatg taatcttttg gtgtatgaca
    15781 ttaataaatc tgctgtggat gaacttatat catgtggagc aaaatacgca agtgttttag
    15841 aaatgggtca agaatgtgat attgtattta caatccttcc aaatggaact attgtgcagg
    15901 atatattgtt tgggatggat ggattggcaa aaactttaaa agaaggaagt atagtagtcg
    15961 atatgagttc agttactcct acagaatcaa tattgtgtgc aaataaatta aaagatatgg
    16021 gcttagaatt tatagactca ccagtaagtg gaggggagcc aaaagcgatt gatggtacac
    16081 ttgcttttat ggctggtggt aaggaagaaa tttataagaa agtagagcca ttttttaata
    16141 taatgggaag ctcatctatt ttaattggag ataatggtag tggaagtgtt acaaaactta
    16201 caaaccaagt aatagttaat ttgacgatag cagcagtttc tgaggcattt gtacttgcag
    16261 caaaagcagg tgcagaccca gaaaaagtat ataaggcaat aagaggaggc ctagctggta
    16321 gtacaatatt ggatgcgaag ataccaatga ttatgaatcg tgattttaaa ccaggtggaa
    16381 aaatatctat taatttgaaa gatattaaga atgtaatgca aacagcacac aatttagatg
    16441 taccgctacc tatgacaagt caattattag aaattatgca gacattaaaa gtacatggac
    16501 atttggaaga tgaccatagt ggaatagcac agtattttga aaagttggca ggtgttgaaa
    16561 taaaaaaaca caataattaa tatttaaata tatcaatata attaaataca tattattagg
    16621 attagattat ataaatacag cgaggttaag ttatcatagt aaaagaaaaa taaaattgat
    16681 aaataataaa gtagaatgat aaaggggtaa agattatgat tacaggatat ggacttttga
    16741 tagcatttgt tatatctata ggaattttac ttgtatcgat tattaaattt aaagtaaatc
    16801 cgtttttggc attactgata acatctataa ttacaggctt tatggttaga atgcctataa
    16861 atgaaatatc aaccacaata tcgactggat ttggaaatac attaggtagt ataggtattg
    16921 taattggact tggaataata tttggaaata ttctttcaga atcaagagca acagaatcta
    16981 ttgcaaaggg gttgttagct aagactggag aaaaaaacag tgctttagca gtaactacag
    17041 caggtttttt aatttcaatt ccagttttta tggatgcagc ttttgttata atgatgccta
    17101 taattaagta tgtttcgaga gtaactaaga aatctctaat ggtttttgta tgtgcattag
    17161 gagttgggac tatagtaggt catgcacttg taatacctac accaggacct ttagcagttg
    17221 cagcaaatgt aaatgcaaat gtaggtagtt ttatacttta ttctataatt gttgcttttc
    17281 cagcagctct tataggtgga tggatttatg gaaaacgttt tgaaaaatat ccagcttatg
    17341 ctattgatga gaatgataga gaaaaaaatt tagagcaggg aaaagatagc ataaaaattg
    17401 aagatgatga ttcttctaaa gtaccaggtt ttggaatttc tatgttttct ttattgttcc
    17461 caatacttct tatacttata tcaaatgtat ttagtatgtt cttagaaaaa ggttctacaa
    17521 tgagtggagt acttgcattt ataggagata aaaacatagc aatacttctt ggaatattgg
    17581 tggcaatagg atttttaaag aaatacataa ataaacctat gggagatgta gttattgaag
    17641 ctgctgattc agcagggcta atattattga taacagggtc aggtggtgcg tttggcagtg
    17701 ttataaatgc aagtggtata ggtaacttct tagttgatac tatgtctggt cttagtatat
    17761 cagttgtagt gcttggattc ttattaagtg ccttgcttag aatttctcaa ggttctgcta
    17821 ctgtagcctt agtaacaaca tcatcaattt taggacctac aattttggca actggaatgt
    17881 caccagtttt agtagggctt gctatatgtg ctggaggagt tggtttctcc cttccaaatg
    17941 attctggatt ctgggtactt tcaagattta gtggattatc agttaaagat actttaaact
    18001 cttggacaat tggaggaacc attgctggag ttgtagcatt tataatggtt ttattgctta
    18061 gtgttataaa tggaattata ccacttccag gtctataaat aacaaactat tgtttatatt
    18121 atataaaatt acatttagac tatacaaatt aaataaaggg gcgttttaaa tgagagaagt
    18181 aaaaccaatt agtatagata tacttaatac ttttaaacaa gtagatgagg acagattaaa
    18241 taaacttctt gcagatgagc ttaagcattt agatagaaaa attgttgtat tagatgatga
    18301 cccaacaggt gtacaaacag ttcatgatat ttctgtttat actgattggg ataaggatag
    18361 tatggaacaa ggttttaatg agaaaaatag tatgtttttt atacttacta attcaagggg
    18421 atttactgtt gctcaaacta caaaagcaca taaagaaatt tcaaagaata ttgtggatgt
    18481 atcaaagaaa gttaataagg attttataat cataagtcgt agtgattcta ctatgagagg
    18541 acattatcct gttgaaacta atttattaaa aagcgaagta gagagattat cggggaagtt
    18601 atttcagggt gaaataataa tgccattttt taaagaaggt ggtaggttta ctatagataa
    18661 tgtacattat gttaaagaag gagaagtact tgtaccagca ggaatgactg aatttgcaaa
    18721 agataaatct tttgaatata aatcatctga tataggtgag tggtgtgaag aaaagacaaa
    18781 aggaaagtac aaatctagtg atatgatata tatttcgtca aaagaactta gagagctaaa
    18841 tattgaagct ataacagaaa aacttaaaaa agctgaagga ttcaataaaa ttattgtaaa
    18901 tgcaaccgac tatgtagatg taaaggtatt tacaattgcg tttatacgag cagtaaatag
    18961 tggtaaggaa tttatgttta gaagcgctgc tgccataaca aaaatacttg gcggagtgag
    19021 tgacaaggaa ctacttacta aggatgaatt agtatcaaaa ggtaatacca atggaggaat
    19081 tatacttgtt ggttcacatg tgaataagac tacccaacag ttagaagaac ttaaaaattg
    19141 taaatatcca atagaattta tagaatttaa tcaacattta gttcttcagg aaaatggtct
    19201 aaaaaatgag gtaaaaagag ttataaaagt tgtagaggaa aaaataagca gtggtaaaac
    19261 tgttgctgtt tatactagac gagagcgttt tgacttggat acgaatgata aagataaaca
    19321 gcttatgatt tctgtagaaa tatcagatgc agttaccagt ataattggta tgcttaatgt
    19381 aagaccaaat tttattatag ctaagggtgg aattacttct agtgatgttg gtactaaagc
    19441 actaagagta aaaaaagcta cagtaatggg acagataaaa cctggtatac cagtatggat
    19501 gacaggcaaa gaaagcaaat ttcctaatat gccatatata attttccctg gaaatgtggg
    19561 agagattagc acacttagag aatcagttga gatattgatg gggtagtata ttagtgtatt
    19621 aaagtttaaa cgcttaaatt tactgtgtta aaagtaaaat aaactaaaat tataaattaa
    19681 gattaaaaat aatagtatat taattagggt ttagagtatt ttacaaaaaa tattctaaac
    19741 cttaatctta tgtattagta tttttattat ttaacttatg tgtttatttt attatttatc
    19801 ttttttaatt gatttaatat tttctttgat tttatttaaa atatctgtat gcttattaga
    19861 gcttatgttg gggaatgctt tgattatacg tctatgaagt aatttgtggt tagacagcaa
    19921 agtattaagt ttatttttta gctcatcaat acgattttta gcatagcgat aattttcttt
    19981 ttctttaagt atttcaaacc tatcttccaa agtatccagt ctattttcaa ctaaagattc
    20041 aagtttgttt tctatagttt cttgtgtaat aagtttgtag tattctttct tatcttttaa
    20101 ttccttaatt ataagattga tttgttttaa cttaccattt aattgtaaaa ttgtatggac
    20161 tgtaataact aaatctaaaa caaaataaat ttctatagat atagcaaata taaatagcac
    20221 atttttagag atagagttaa taaaatcaac cacaatagga tgtactataa gcattacaat
    20281 caatgataat acaccaaaca taagtgaatt ttccagacaa actcttccat gaatattaaa
    20341 tttatgttta gaataatccc accaagtcat attaaatatt ttttctaaaa taaagctcgt
    20401 acagtactcc attatgctag ttataattat actaaacaaa aataacaaag gaatattgtg
    20461 tacaaaagga gtcaaggtag caactattgc taatgctcca aatccatata tagggcaaaa
    20521 tggaccattt aaaaaacctc tatttacaaa ttgtttttgt aaaactgaac aatatgtagt
    20581 ttcacaacac catcctataa agctataaat tacaaaataa agaaattgaa tataaaatgt
    20641 actcataagc acctcatttc tagttagtat aacactatat actcgatatt ttatttttat
    20701 gattaattta ctaaattcaa tttacatatt gattatacca atttatagaa atttatcaaa
    20761 tttaattaca gttagaacat tataaacatg gttataataa tgaaatttta tttaaaataa
    20821 ataagggtgt caagtataat caaactaata gttgtagata ctctacacta tgttagttta
    20881 attacattta acacccttat atttatacta tgactattta cattcgaaac tagagcaaca
    20941 tgtttcaata taaaggttta ctctatcagt ttcaggagca accaatatat tatcaagtgt
    21001 acataagcta ccatctttgt tgtatttaca attttttaca gtacaaacta attcctcaca
    21061 atttctatta ttaacgttgc tagataaatt actataaata gattcatcta aaaatgaact
    21121 acaacaagta tgtctgtcat tgtgagcttt ttcaccattt ataaaaattt tatctgcata
    21181 acatatatgg ctgttgttat gtgaacagtt aattacatta cagtttattc tttccaaagt
    21241 tattacctcc taaacgatta tttattataa attatgtcca tataattaca agaatataac
    21301 cattaagaga aaactacctt ttagaaaata tctattctta gagtaatttg aaatataaat
    21361 atattttctt ctaaaattta cctaaattgt ataaaaactt acagcttgga gaaaataata
    21421 gaaaaaggag gcgtaatcaa tgtttgaaaa gaagaaaaaa gaattatttc cttggaaaat
    21481 atctgcctta atactggttg gagtatttgt attaagtagc ggagtttata tagggacaag
    21541 attaaaacac aatggagttc aaactggtga agttgtaagt actagtgcga atatgaatga
    21601 aaagtcaaaa gaagcttttg gattaaataa aaattgtgaa gtttggcttc aaagtgaagg
    21661 cgaaaatgaa actaactata agagaacact tacaatggca ggaatagttc cagagtcatt
    21721 attagataag acgaagtcag aaatagtatc atattttaaa aaagaatatc caactaaaaa
    21781 aataaagagt atggataaaa atgaaatcat attggtagaa tctaaatata gtgataataa
    21841 agatgtacaa gcaatggatg ttactataaa aggtaaatat actattgaaa atgataatgg
    21901 aaatatagca ctatataaat atgatgatag tggaagcaaa acattagtag aaaaaacaaa
    21961 aataagagta gattcacttc ctaaaacagt tcaagatgaa atcaaaaaag gtgtagttat
    22021 ggatactgaa gaagaggcct attcaagatt agaagacttt gcaagttaat agtaaattaa
    22081 tattatttta agttaaatac taagtaaatt aataataaat aatttaatat taaatagatt
    22141 agtattaaaa ttgtagtcac atttaattcc tataaaaata caataattta tttgtaataa
    22201 atataaaagt tatattgagg taaaatcttg ctttgatata acttttttat ttgattttat
    22261 attttacata gagcatttta tgatttgtta atattattta tgatataatg atttagaaca
    22321 taagttttta tgagaaatac attttggtat ttcttttatt tttaggggga attttaaaaa
    22381 atgataatat tggggattga cccaggtata gccatagttg gatatggtat aattgaatac
    22441 aaaaatagca agtttaaagc aatcgattat ggagcagtta caacacctgc ccatatgaat
    22501 atatcgagaa gattggaact tgtgtataaa ggaattgata caatagtaaa gaattacaat
    22561 atagatgaag ttggaatgga agaattattc tttaacaaga atgtaaaaac agctataaca
    22621 gttgcacaag ctagaggtgt tactatgctt gcatgtgctc ataatgggaa gcctgtatat
    22681 gaatacactc cacttcaagt aaaacaaggt gtagttggat atgggagagc agataaggca
    22741 caagttcaac agatggtaac ttcattttta agtctaaaaa aagttccaaa accagatgat
    22801 gttgcagatg ctttagctgt ggctatttgc catgctcatt caaacaaact tgaaaaaact
    22861 ctaaagaata taggtggtaa gtatgtatag ttatataaaa ggtacagtag aagaaattta
    22921 tatagacagt atagttgttg aaaataatgg gataggatat aaaataaatg tatcatccaa
    22981 tacaataatg aatttacaag tgggagaagc cacaaaaata tatactaaat taattgtaag
    23041 agaagatgat atgagtcttt gtggttttgt aagtagggaa gaacttaaga tgtttgaatt
    23101 actaacatct gtatctaaaa taggacctaa agtggcgctt tcaatactat catttgcatc
    23161 accagcacag ttaggagcat atattttaag tgaagacatt ggaaaattat ccaaagctcc
    23221 tggtgttggt aaaaagacag cagaaagaat cgtcttagag cttaaggata aggtagacaa
    23281 aaataacata gagtttgaac caacgttatt atcacaaaaa cctaccttga tatcacaaga
    23341 tgagtctgta gatgcacttg tagctttagg ctatactttg tcggaatcaa aagaggcagt
    23401 tcaaaaatgt aaaaaagatg gtatgaatac agaagctatt ataaagaaag cacttacata
    23461 tataatgagt aaatccttga aatagtattt ggattttata atatttggat tttaaaacaa
    23521 tatttaagga gtttatgtaa atttgaaaat atataaagta gattttcaaa agtacgagag
    23581 gaggggtata aatgcaaggt tttgaagatg aaaataggat aataacttct acaatgaaga
    23641 tggaagatat agatatagaa aacagtttaa gaccaaaaac tttagaagat tatttgggtc
    23701 aagaaaaatc caaagaacaa cttagtatat ttatagaagc agctaaatcg agaaatgagc
    23761 agttagacca tgtgctttta tatggaccac caggtcttgg aaaaactaca ctagcaagta
    23821 taatagcaaa tgagatggga gtaaatttaa gaattacgtc aggacctgca atagaaagag
    23881 ctggagattt ggcagccata cttacgaatt taaatgaaaa tgatgtttta tttatagatg
    23941 agattcatag aatcaataga agtgttgaag aagttttata tccagcaatg gaggattttt
    24001 gcctagatat aataataggg aaagggccat ctgctagaag cataagactt gaccttccta
    24061 aatttacact tattggagct actacaagag ctggtatgct tacaaatcca cttagagata
    24121 gatttggtgt tatttgtaaa ttggactatt atacagttga tgaactttca aaaatagtac
    24181 ttaggtcttc aagcatctta gatgcagaga tacagtctaa tggagcatta gaacttgcaa
    24241 aacgttcaag agggactcca aggatagcaa atagattact aaaaagagtt agagattttg
    24301 cacaagtcag agctgatgga aaaataactg ataaggtggc aaaagatgct ctggaacttt
    24361 taggagtcga tagtttgggt ctcgattttg tagatgagaa gcttcttatg actataatag
    24421 aaaaatttag aggaggacca gtgggtttag atacattggc agcttctatc ggagaagata
    24481 gaaatacaat agaagatgta tatgagccat atttgcttca attaggattt ataaatagag
    24541 gtcctagagg aagagtagcc atgccattag catatgaaca cttaaaaatt ccatatccta
    24601 atgaaaaata aaaatagatt atacgagtat ttataaaata aaagagtcat taaattaaaa
    24661 tatttctaaa atggctaaaa attcaaacat atccacaaaa tggtaattat atctgtagat
    24721 actgtggata aacacgataa aaattgtgtg tattgtatct atatattgtg gataagacat
    24781 gtatatagtg gaaaaatatc tatatattgt ggatatggtg tctgttaaaa atagcaattt
    24841 cttgatagat tatggtaata ttgtggaaaa taagttgttt ttttgatatt attgtagctg
    24901 gagaaatttt aagagaggaa gtttaacatt gaaaacaagt gattttaaat ttgacttacc
    24961 acaggaactt atagctcaag ttcctataga agatagagct agctcaagat tgatggtatt
    25021 agataaagag acaggtaata ttgaacataa agtatttaga gatataatag aatatttaaa
    25081 tccaggagat tgcttagtat taaataatac tagagttata ccagctagat taataggaga
    25141 gaagcttgaa actggaggta aaatagagtt tttattatta aaaagaacag aggaagatac
    25201 ttggcaggct ttagtaaaac caggaaaaag agcaaaagta gggactaaat tttcttttgg
    25261 aaatggaaag ctaattggag aagttgtaga tttatcagat gaaggttcaa gaattataaa
    25321 attccattat gatggaattt ttgaagaaat attggatgaa cttggaaata tgccactacc
    25381 tccatatatc actgctagac tagatgaaaa agaaagatac cagactgttt attcaaagca
    25441 taatggttca gcagcagcac ctacagcagg attacatttt acagaagaat tgttaaataa
    25501 aataaaagaa aaaggtgtag atatagcttt tgtaactcta catgttggct taggaacttt
    25561 tagaccagta aaagtagagg atgttttaaa tcacaagatg cattcagaat attatatggt
    25621 tagccaagaa gcagctgaca aaataaatag agcaaaggaa aatggaaaaa atgtaatatg
    25681 tgttggtact accagttgta gaactataga atcagcttgt aatgaggatg gaaagatgaa
    25741 agaaactagt ggatggacag aaatatttat atatccaggt tataaattta aagttttaga
    25801 taaacttatt actaattttc acttaccaga atctacacta ataatgttag taagtgctat
    25861 ttgtggaaag gataatgttt taaatgcata taatgaggca gtaaaagaaa gatatagatt
    25921 cttcagtttt ggagatgcta tgattataaa gtagggaggt aatggatgca tagtttacaa
    25981 aaacaaaaaa agaataaccc tatattaaag attgctttta tatttgaacg tgttttagca
    26041 gtagtagttc taatagcagt atttttagga actatagatg ttctaagact tatgtgggga
    26101 gcatatatag ttgattttca aaatccagta caatacagtc agcttaatga ttttttagca
    26161 caaatattac ttttagtaat aggtgttgaa cttgtagtaa tgctatctct tcatatacca
    26221 ggagcattta ttgaggcatt actttacgca atagctagaa aaatgttgct actgcctaaa
    26281 aatgaaggta tgatagatgt tttaatagga gttatagcta tagcaggact atttgctata
    26341 aaaaagtttt tagtaacaaa agacaagagt gctcttaata taatgagtat tcatgatgaa
    26401 gaagcaatta aggaacaaca gaacaaagaa gacgaagata aatctattta gacaataaat
    26461 tatatgaata gcttattaca aatatatctt aagaaataat gagaaataaa tatgattaag
    26521 gagaacttta tgtacgcagt aagatatgaa ttgataaaga cttgtaagca gagtggtgca
    26581 aggttaggaa gattacacac tccacatggg attattgaaa caccaatatt catgccagtt
    26641 ggaactcaag caactgtaaa atctatgaca ccagaagaat taaaagagat tggttcacaa
    26701 ataatactta gtaacacata ccatttatat atgagaccag gtcatgaact tataaaaaga
    26761 gctggtggtc ttcataagtt tatgaattgg gataaaccaa tacttacaga tagtggaggg
    26821 ttccaggtat ttagtctagg gcctttaaga aagataaaag aagaaggtgt agaatttagg
    26881 tcacacttag atggttcaaa acattttcta actccagaaa aagctatgga aatacaaaat
    26941 gcattgggtt cagacataat gatggcattt gatgagtgtg caccataccc atcagataga
    27001 gaatatgtaa aaaattcttt agaaagaaca acaagatggt taaaaagatg taaagatgcc
    27061 cataataata ccgacaagca agctttattt ggtataatac aaggtgggat gtataaagac
    27121 ttaagggaac aatcagcgaa agaaataact aatattgatt taccaggata tgctattggt
    27181 ggacttagtg ttggagaacc aaaaccactt atgtatgacg tgttagagca tacaactcca
    27241 cttatgccaa aggataaacc aagataccta atgggagttg gtagtccaga tgacttagta
    27301 gaaggtgtta taagaggagt agacatgttt gattgtgttc tacctactcg tatcgctaga
    27361 aatggaactg caatgactag ccaaggtaaa gtagtagtta gaaatgcaac ttatgctgaa
    27421 gactttactc cacttgaccc tgaatgtgat tgttatgctt gtaaaaacta ttcaagagct
    27481 tatataagac accttataaa agcaaatgaa atattgggtg caagattaat aactactcat
    27541 aatcttcact ttttattaaa tttaatgaaa caaataagac aagcgataat ggaagataga
    27601 ttacttgatt ttagaaatga attttttgca aaatatggat atgaaatata ggttttgcac
    27661 tattaattat aatcatttta atatataata ttcaatagta ctcaaagata gagaaagtaa
    27721 attgttatat aaacatttat tatgatttac aaaagagtat aatttacaaa ggagtgaaaa
    27781 attaatgcca cagcaacaaa taattatgag tataggtcta tgggtagtag taatcgcaat
    27841 cttttatttc ctaatgatta gacctcaaaa gaaaaaagac aaacaattaa aagaaatgag
    27901 aagtagccta agtgtaggag acaaggtcat tacaataggt ggaatagtag ctaatgtagc
    27961 taaagttgaa gatgatgttg taatattaga attaggacca aatagaacta aagttccatt
    28021 tgaaaaatgg gctataggaa ctgttaaatc taaaaaagaa gaaatagaag aagactaaat
    28081 tagtaattta aaagcctttt ctcgaatata tattaatttg agaggaggtt tttttatggg
    28141 aaaatctaat tatattttta aaggtctagg atatgcatat ataataactt tggctgtttt
    28201 attagtgtat aacttatttt tgacatttac agatattggt ggagataata taactatggt
    28261 ctcatcattt ataactacta tttctgcagc tataggaggg ttttacactt caaaacacat
    28321 gaaagaaaaa ggacttatgt atgggctttt agtaggactt ttgtacatag tatgtatttt
    28381 tttgactgtg tttttagcac aagaaaagtt tgtatttgaa gtagggatga tttataagtt
    28441 attgttaata tcagcagcag gaggcattgg aggcgtattg ggtgtcaact ttaaatagag
    28501 tttatattaa agtagtttta tgatataatc aataggaaac aattagaaat ggaggcgttg
    28561 aaaatggaaa ataaacatgt aaaaacttta tctcaagcta ctttaaagca aagtgctgca
    28621 aaaggtggat gtggagagtg tcaaacttct tgtcaatcag cttgtaaaac ttcttgtaca
    28681 gttgcaaatc aagagtgtga aagatagaaa attattgatt tagttgtttg taagagggta
    28741 tctcaaagtg attcttttat cattatgagg tatcctcttt tattttcaat tattatataa
    28801 aaatatattc tatttatgta aaattaaatt tgatttctta tcgtttaatt atgcaaaaat
    28861 atctaatcat ataagagcat tcattttaaa gtagctactc ttatatttta ttttttatca
    28921 attacatgta ttagaataat ttttaatttt aaactctaat ttatgat
//
LOCUS       DAEMHW010000033        28908 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEMHW010000033 DAEMHW010000000
VERSION     DAEMHW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.033"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(319..1074)
                     /locus_tag="KPK67_001242"
     CDS             complement(319..1074)
                     /locus_tag="KPK67_001242"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG2458235.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1514..2503
                     /locus_tag="KPK67_001243"
     CDS             1514..2503
                     /locus_tag="KPK67_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBG2458236.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(2923..3639)
                     /gene="dapD"
                     /locus_tag="KPK67_001244"
     CDS             complement(2923..3639)
                     /gene="dapD"
                     /locus_tag="KPK67_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBG2458237.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(3904..4653)
                     /locus_tag="KPK67_001245"
     CDS             complement(3904..4653)
                     /locus_tag="KPK67_001245"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG2458238.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(4720..5607)
                     /locus_tag="KPK67_001246"
     CDS             complement(4720..5607)
                     /locus_tag="KPK67_001246"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG2458239.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(5724..6725)
                     /locus_tag="KPK67_001247"
     CDS             complement(5724..6725)
                     /locus_tag="KPK67_001247"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBG2458240.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(7150..8031)
                     /locus_tag="KPK67_001248"
     CDS             complement(7150..8031)
                     /locus_tag="KPK67_001248"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG2458241.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            8354..9547
                     /locus_tag="KPK67_001249"
     CDS             8354..9547
                     /locus_tag="KPK67_001249"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBG2458242.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            9753..10871
                     /locus_tag="KPK67_001250"
     CDS             9753..10871
                     /locus_tag="KPK67_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBG2458243.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            11002..11247
                     /locus_tag="KPK67_001251"
     CDS             11002..11247
                     /locus_tag="KPK67_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBG2458244.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            11234..11980
                     /locus_tag="KPK67_001252"
     CDS             11234..11980
                     /locus_tag="KPK67_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2458245.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENIFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            12263..13138
                     /gene="hslO"
                     /locus_tag="KPK67_001253"
     CDS             12263..13138
                     /gene="hslO"
                     /locus_tag="KPK67_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBG2458246.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            13529..14329
                     /locus_tag="KPK67_001254"
     CDS             13529..14329
                     /locus_tag="KPK67_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG2458247.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            14856..17096
                     /locus_tag="KPK67_001255"
     CDS             14856..17096
                     /locus_tag="KPK67_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG2458248.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNICEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGIKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            17510..19045
                     /locus_tag="KPK67_001256"
     CDS             17510..19045
                     /locus_tag="KPK67_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG2458249.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVTIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            19061..19822
                     /locus_tag="KPK67_001257"
     CDS             19061..19822
                     /locus_tag="KPK67_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2458250.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDNPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            20165..20221
                     /locus_tag="KPK67_001258"
     CDS             20165..20221
                     /locus_tag="KPK67_001258"
                     /inference="COORDINATES: protein motif:HMM:NF036872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458251.1"
                     /translation="MLMEALANGINKTKEAEE"
     gene            complement(20271..20795)
                     /locus_tag="KPK67_001259"
     CDS             complement(20271..20795)
                     /locus_tag="KPK67_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HBG2458252.1"
                     /translation="MIAMQYKILLPDDYDMDNIRQRVKNNGYKTDGFDDLMFKAYLIK
                     EKNGISESHNEYSPLYLWKNSGGMNKFIFDGFYNNILQSFGWQKINIGIPLLCDLGKE
                     FNKSQYAMEIENDILPTNQMSPLSFLKTSDRCLGKVLIYNPDKWKYVEYHFYEREPEK
                     SLKNKIYQILHLSV"
     gene            20896..21774
                     /locus_tag="KPK67_001260"
     CDS             20896..21774
                     /locus_tag="KPK67_001260"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012354.1,HMM:NF015431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2458253.1"
                     /translation="MVDIIFSDGGYVMESLELRIFREVARTKNISRAAKNMGYVQSNI
                     TAHIKKLENELNTTLFIRYNKGVTLTEDGEKLLQYAEQIISLLDKALYTFHRDIRQLK
                     IGATQTIAGYLLPQCLTEYQNRFPNINISIKTSNQQDMDKQLRQKLLDCVITNDPHVF
                     TDAQCIFKTREFLSIIAPKFCESISQIWQLPIVVNNLKTCPYRKIVTDLFLRESASFK
                     IIELDTVESIINTVAAGGGISLLPKNILRNKLTINEFDIPELEPTSINIWIHKDSFPS
                     DFITFKEIIERLTCYK"
     gene            22146..22397
                     /locus_tag="KPK67_001261"
     CDS             22146..22397
                     /locus_tag="KPK67_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBG2458254.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            22509..23288
                     /locus_tag="KPK67_001262"
     CDS             22509..23288
                     /locus_tag="KPK67_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2458255.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(23423..24073)
                     /locus_tag="KPK67_001263"
     CDS             complement(23423..24073)
                     /locus_tag="KPK67_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458256.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDILIIAFPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(24070..24573)
                     /locus_tag="KPK67_001264"
     CDS             complement(24070..24573)
                     /locus_tag="KPK67_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2458257.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(24622..25137)
                     /locus_tag="KPK67_001265"
     CDS             complement(24622..25137)
                     /locus_tag="KPK67_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG2458258.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            25417..25941
                     /locus_tag="KPK67_001266"
     CDS             25417..25941
                     /locus_tag="KPK67_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2458259.1"
                     /translation="MHEQIDCCERKKRTIDIKGENMRELLKWVSIINRYNTNYIDRKL
                     ADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVK
                     DKRTWHLYPTEKSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMN
                     EEAKLEAKENGRKI"
     gene            25925..27328
                     /locus_tag="KPK67_001267"
     CDS             25925..27328
                     /locus_tag="KPK67_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2458260.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHSKIAEV"
     gene            complement(27801..28112)
                     /locus_tag="KPK67_001268"
     CDS             complement(27801..28112)
                     /locus_tag="KPK67_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458261.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIFISKIGNKT
                     K"
     gene            complement(28168..28761)
                     /locus_tag="KPK67_001269"
     CDS             complement(28168..28761)
                     /locus_tag="KPK67_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2458262.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT        10647 a         3658 c         4683 g         9920 t
ORIGIN      
        1 atgatttcat ataccaataa attaggataa tatttaatag gtttatatga tgaaaagatt
       61 ataaaataat aaacaatatg attccatata ccaataaata ggataatatt taataggttt
      121 atatgatgaa gagattataa aataataaac aatataattc catataccaa taaataagat
      181 aatctttaag aaaaatgatt atatttaaaa ggtaagcata gaatttataa acttttagaa
      241 tagaaatctc aatcaattag atattcaaat tgatgaatat gaatacctaa atagattgag
      301 atttattatg tacaaaactt atcctagtat atctgacatt ttataaagcc cagattgctt
      361 accaattaaa aatttagctg caactataga accgtttgca aatacttcat cagattgaga
      421 atgatggctt aatgtaagaa cttcattatc acctgcaaat acaacatcat ggtcgctgat
      481 tatattgcca cctcttatag agtgtattcc aatttcattt ggttttcttt tacaatctct
      541 accatatcta ccatgtgtat tttcactttc aggcaagact tctctaattg cattagctat
      601 catagtagca gttccactag gggcatcttc ttttctattg tgatgttttt ctatgatttc
      661 aatatcaaaa ccttctaaca tctttgaagc atccttaact aattttatta atatattcac
      721 tccaagagat gtatttgatg ataataatat aggaatattt tttgatgctt cttctatttt
      781 atttaagtca ttttcatcat atccagtagt agctaatact acaggtgtat tattttttag
      841 agcatatgac aatattggct ctaaagtctc atgatgtgaa aagtctatta ttacatcagt
      901 tttttcagtt acatcagtca ttgttttgta aagctttgta tcactatgat gacctttttc
      961 atcagcagac ctagctacac cacaaaccaa ctctaattca ttatcttttt ttacacattc
     1021 tgctaaaact cttcccattg ttccattata gccactaatt ataactttca acataaattc
     1081 ctcctatgaa attaataaaa atttgttata tttctataag tttattttat cactaatata
     1141 atttatataa aataatatta gtgataataa gcttacaatt tatcttacat taaaaaattt
     1201 aagtggattt ttcaaaatat agataaattt ctttttcatt gtataaatga taaaaatata
     1261 tttgacttta taccaaaaag agtgccccat gaactaaaaa gttaattttg aaataccata
     1321 ttcatatatc atatgattta tttggaaatc aatattaata cattaaaata actaaataca
     1381 tgtatgcata aagatgtata aaaaatatat aaacatatag tattaatttt aaattcaaca
     1441 atttaattgt taaaagtgtt attattaaat tagagatatc ttaacttaat atactgagat
     1501 gggagtgata gatatgttaa aagaagaaaa aacttatcaa gagaaagacg caaaagtatt
     1561 caatggagtg gcagctttga taatgatttt attattttta gtagtttcaa taggtatcat
     1621 ggtttttggg atagtaaaac ttgatagtgg atttactgga ataggttcac ttatgttaat
     1681 tttgggactt gtatttatag tagtagattt tatcctattt tttgggctaa aaatgattaa
     1741 tcctaaagaa gctatagttc tagttttgtt tggtaactat tatggaacta taaaaaaaga
     1801 agggtactat tgggtgaatc cattttgttc agcaataaac ccagcagtat caagaattaa
     1861 tgtatcgaaa tcttcatctg atgaaaaggt agatatatca actaattcaa ggggcaaaaa
     1921 agtatcttta aaaacaatga ctcttaataa tgaaaagcag aaagtcaatg atttattggg
     1981 aaatccaatt ataataggag tggttgtaat ttggaaggtt atagatgcaa caaaggcagt
     2041 atttaatgtg gataattata atacattctt atctattcag tgtgattcta ctattcgtaa
     2101 tgtttcaaga ctttatccat atgatgtatc tgaagatgga gatgaaaaat ctcttcgtgg
     2161 tagtagtcag gaaatagcag atagattaaa agatgaatta caatcaaggg ttgacattgc
     2221 aggaattgaa gtgtgtgaag tgagaattac tcatctttct tatgcaccag agattgcagc
     2281 agcaatgtta caacgtcaac aagcagaggc tataatagca gcacgtaaaa agattgttga
     2341 gggggctgta agtatggtag agatggcatt gactaattta agtgaaaata atgttgtgac
     2401 tttggacgaa gaacgtaaag cagctatggt aagtaatctg ttagttgtgc tttgtggcaa
     2461 taaagatgca caaccagtag taaacagcag ttctatttat taaattataa aaagtttaat
     2521 agtttatttt attgatatat agaaaattta gtagttcatt ttattaaatt atagaaagat
     2581 taatggttcg ctttattgaa ttataaaaaa tttagtagtt cattttatta aattatagaa
     2641 agattaatgg ttttcacttt attaaattat aaaaagttta atagtttatt acattgatat
     2701 attgaaaaat tgaaggaaat aaaaatactt gtcagcaaga aataaaaatg atataagaat
     2761 gaataagaga attgtaaaaa aataaaatga ttataaataa aagataaaaa tattgtaaaa
     2821 aagagggtgc ctctggtgaa ttaaattcac acagagacac ctttttaatt aattttttaa
     2881 attagtatat tattgtttaa ttaatagcat agtactattt aattaatcta aatttctaag
     2941 gtcttccata agcttagttt tgtcagccgt tttttcatct ttcattttta taactttagc
     3001 aggagaacca gcaacaacgg caccagcttc tacatcagta gttacaacag caccagctgc
     3061 aacaacagca cctttaccga ttctaacacc ttcaagaata actgcattag ctcctattaa
     3121 aacatcatct tcaactataa caggagtagc agaaggtggc tctaaaacac cagcaacaac
     3181 agcaccagca cctaagtgaa catttttacc aagagttcct ctagctccaa taacagcatt
     3241 catatctacc atagaacctt caccaataac agctcctata tttataacag ctcccatcat
     3301 tacaacagca tttttttcta tagtaaccat gtctcttatt attgcacctg gctcaattct
     3361 tgcatgttca cttaaagtat tttttagagg aatagcagaa tttcttctat catattctac
     3421 atgagtatct gttatatcat ttccatgttt atctaatact tccattatat cctcatagtc
     3481 acctacaaat atataagaac cattacttcc aaatacctta aatttatcag ttgattggaa
     3541 gtgtaaagag ttagtattta catatacctt aacaggtgtt ttctttttaa cttcttttat
     3601 ataccttgct atttcataag catcgtttaa atttaccatt tgttaattct ccttgtattt
     3661 cattatattt cttttattat atacttattt gtcaatttta gtatatactt actttttatt
     3721 atttatattt tattttagtt agtttttatt gtatagacat agtattaaat aaatttatta
     3781 tatatgtttg atttgtattg cgtattatta tatctatatt gcatatgttt gatttgtatt
     3841 gcatattatt atatctatat tgtatatgtt taatttatat tgtgtattat tatatttata
     3901 ctattatccc aacatgctgt ccatattata aaatcctggt ttttgcttaa ctaagtattt
     3961 ggcagcagct atagaaccac ttgcaaagat accacgagat tgagcttgat ggcttattgt
     4021 aagaacctca ttgtctccag caaatagagc atcatgttcg ccaacaatat ttccacctct
     4081 tatagcatga ataccaactt catttggttg tctttttgca ctacgaccat atctaccata
     4141 attattttca acgtttggca aaacctcttt tatagcgtta gcaatcataa cagctgtgcc
     4201 acttggggca tctacttttc tgttgtgatg tttttctatt atttctatat caaatccttc
     4261 tagtaattta gctgcatctt taaccaattt tactaataca tttacaccaa gagacatatt
     4321 atatgattgg aatacaggaa taactttagc cgcttcacgt attttgttca tttcgtcatc
     4381 attatatcca gtagtagcaa ttactaaagg tgttttagtc tttaaaacgt aagataagat
     4441 attatctaaa ttagagtgat gagaaaaatc tataactaca tcagcttcct caactatagt
     4501 actcatatct tcataaattt taaggtcgcc ttcatagcta tccagacctc tagctgcaac
     4561 acaaaccaat tctaattcat tatcttctct tatagtatcg gctaaaatct tacccatttt
     4621 tccatcataa ccagttgcta taacttttaa cataatttcc tcctatgatt aagggctaac
     4681 ttttgaccaa acttactttt taccatgcat taatctttac tatgcattaa cttttagtcc
     4741 aaagttaact aattctttct ttaatacttc taaatttgct gggtccattt ctgctagagg
     4801 taatcttaaa tctccaacat tgaatcccaa taaattcatt gcagtcttaa ctggtatagg
     4861 atttacttct ataaataaag ctgcgattag agcatccata ccaagttgta attttctaga
     4921 accttcaata tcgccttcaa agaatttagc taccaaatcg tgagtttctt gagggcaaac
     4981 attagcaagt actgatataa ctccttgacc tcctaaagat aataaaggaa gtatagtatc
     5041 atcattacca gagtagatag caaagtcatc tggaactaaa ctagctattt ctgcaacctg
     5101 tcctaggtct ccactagctt cttttacagc aactatgtta tcaattttag caagttcaac
     5161 tataaggcta ggacttatat ttacacaagt tcttccaggg acattgtaaa gtataattgg
     5221 aagtttaaca ctgttagcta ttgtttcaaa atgacgctta agtccagctt tattagcttt
     5281 attgtaataa ggtgttatta ttaatagagc atctactcct aatttttcag cttcttgact
     5341 taagttaact gagtgcatag tatcattaga accacttcca gctataacag gaactctttt
     5401 attaactttt tcaacaacat acttaatagt agcaagttgt tcttcatcag tcatagtatt
     5461 agcttcacca gtagtaccac aagatactat agcatcagta ccattagcta tttggtattc
     5521 aactaattca cctaatttgt caaaatcaac tttgttatcc tttgtaaatg gtgtaactag
     5581 agcaacagca gaacctttaa atatcatata aaatcaatcc cctctttttt atttttgtgt
     5641 tttatattac agttacttct aatgcttgtt tttgagttac tattaatatt ttacaaaatt
     5701 atatatttat acaagtttgt atattatact aaatcgtatg ataaagccat ttcagctatt
     5761 tgaattgcgt tagttgcagc accttttctt atattatcag caacaaccca aagatttaca
     5821 ccattatcaa cactaaaatc tcttcttatt cttccaacaa atacttcatc tctatcatca
     5881 aattctacag cagttggata ttcgttttta gcaggattat caactactac aacacctgga
     5941 gcttcttcaa gtgtttttac taaatcatct aactcaaatg gattgttaaa ctcaacattt
     6001 atagattcac tatgaccatt ttttactgga actcttacag tagttgcagt tatttttaaa
     6061 tcgtagttat caagtatttt catagtttca tttaccattt tcatttcttc tttagtgtat
     6121 ccattttcag tgaatgaatc tatatgagga agacagttgt aagagatttg atgagggtag
     6181 aatccagtag gattaccgtt cattcctttc tctaaatctt caacaccttt aacaccagaa
     6241 ccagatacag cttggtaagt agagtaaaca actctcttaa ttttgtattt atcatcaaga
     6301 gctttaaggg gaaccatagc ttgtatagta gagcagttag gattagctat tataccctta
     6361 tgatttttta tggcttctgg attaacctca ggaactacta aaggcacatc tggattcatt
     6421 ctccattgac tagagttatc tactactaag attccatttg aagcagctat tggagcatac
     6481 ttttcactaa tacttccacc agcagagaat aatgctattt gtatgcctct atcagtaaat
     6541 gaagtttcct ttaattcttc tactatatat tctttaccac aaaacattac tttagaacca
     6601 gctgatttag aagaagagaa taaaaataga ttttctattg gaaaatttct ttcttctaat
     6661 acttttaaaa atgttctacc taccattcca gtagcaccaa ctatcgctac atttactttt
     6721 ttcatataaa ttcaccccaa ttttaaatta ttaaaacatt attttatttt agttatacaa
     6781 gtaagatatg ttaatatttt tatatatcca aaaactttaa tattatatta acaactttaa
     6841 ttatatacaa aaaacgtaca aaggtatact ttgtacgtct aatttatcac tcaaagtaaa
     6901 ccctgtagct catcacctac aaaaataagt gacagtatta cacatgttat gtgcaatccc
     6961 agaaaatatt attgcaagaa atattttctt cggctgtaaa tcctttttcc aatcattaac
     7021 acctgccagc tactattgga tactcttagt tacagctacc tctaccctag gttaatagga
     7081 tattaaaatt tgattattct aataataata catatttaag aaaaaatcaa cttatttata
     7141 taaatattgc taaaatttaa atccaaaatt tgtcaattct tgttttaaca cattaagatt
     7201 agtttcttcc atttcagcta gaggaagtct taaatcacca acattaaaac ctaaaatgtt
     7261 catggcagtt ttaacaggaa ctggatttac ttctataaat aaggcagcaa ttaaagcatc
     7321 catatcaagt tgtaattttt ttgagccttc aatatcacct tcaaagaatt tagttactaa
     7381 atcatgtgtt tctttaggac atatgttagc aagtacagag ataacaccac ttccacctaa
     7441 tgataatagt ggtagtatag tatcatcatt accggagtat atagcaaagt ctttaggtac
     7501 taatttagct atttctgcaa cttgagctaa gtctccactt gcttccttca cagcaactat
     7561 attatctatt ttagcaagtt cggccacaac agatggcttt atattcactt tagttcttcc
     7621 tggtacgttg tataaaataa ttggcaactt aacgctattt gctattgttt caaagtgaag
     7681 tttaagacct ttctcattag tcttattata gtaaggagtt attattaaaa gaccatccac
     7741 ccctaatttt tcagcttctt gactcatatg aacagacaac atagtattgt ttgaaccagt
     7801 acctgctata acaggtattc tcttattaac tttttcaact gtatatttta ttacagcaaa
     7861 aatttcagat tcactcatag tagtcgcctc acctgttgtt ccacaaacaa ctagtgcatc
     7921 tgtgccattc tcaatgtgat attcgattaa ttcacctaat ttttcaaagt tgacattatt
     7981 atcctctgta aatggggtaa ctaaagcaac agcagaacct ttaaataaca tataatcaaa
     8041 tctccttttt tatctaaatt taatttttta agtacaataa taattatagt acaaattaat
     8101 taattttaat aggtatagtt aatatcttaa ctgtttaaat aagatattta tattatatta
     8161 atattagaaa aattttaaat aaatgtttta ttctcaaaat atgcatatta atttaataaa
     8221 atataccaaa atggaaataa aaaataaatt ttatgagaat ttaataacta ataattaata
     8281 tatttaggtt ataataaata tgtgcgtaaa atctaacaat aagcaataat accgtaaatt
     8341 taggaggaat aatatggata ctttaaagga actttttaaa ataggaagtg gaccttcaag
     8401 ttctcatact atgggaccac aaagagcagc agagagattt aagaatgaaa acccagatgc
     8461 agaaagtttt agagcaatat tatatggtag tttggcagct acagggaaag gtcacttaac
     8521 tgactatata attgaaaaaa ctatagcacc taaaaaagtc gaaattgttt gggaagaaga
     8581 tattataaaa gattttcacc caaatgggat gaagtttgaa gctttagata aagataaaaa
     8641 tgtaacagct gaatggactg tatattctgt aggtggagga actatagcag aagaaggtca
     8701 aagaaacagt aaaagcaata gtatatatca cttagatact atggatgaga tagtaaaatg
     8761 gtgcaaagaa aataataaga ctttggtaga ttttgtgttg gaatgtgaac caaaagacat
     8821 aaaagattat ataaaaacta taaaagatgc tatgagaaaa tctattgatg atggactaag
     8881 tacagatgaa ataataccag gcaagttatt attaaaaaga agagcaagta cattttataa
     8941 tgcatataaa aaagataaga gcttctcaac acttgtatat gcatatgcac tagcagcttc
     9001 agagcaaaac gcttctggta atataatagt tacagcacca acttgtggtt ctgctggagt
     9061 aataccagga atattctttg caatgcagga tttctataat tatgatgacg aaaaaataat
     9121 tgaagcacta cttgtagctg gaataatagg taatataata aaaactaacg cttcaatatc
     9181 tggagcagag gtagggtgtc aaggtgaagt tggagccgct tgttctatgg cagcagcagc
     9241 agtagcatac ttaaaaggtg gaactataga ccatattgaa tatgcggcag aaatagcact
     9301 tgaacatcat ttaggaatga cttgtgaccc tgtatatgga tatgttcaaa ttccatgtat
     9361 agaaagaaat gcaatggcag ctcaaagagc ttatgatgca gctaattatg cattattaac
     9421 tgatggctca cattctgtct ccttagacca agttgttgaa acaatgaaag aaactggtat
     9481 agatatgatg gataagtaca aagaaacagc taagggtgga cttgctaaac atttcttctc
     9541 ttgctaaaaa taaaattttc caataataat tcctctgttt tgatttatga aacaggggaa
     9601 ttattattaa tggtgaatta tttaataatg caaaatattt ttatattcaa tttagctttt
     9661 tatttttaat tagtataaca tgctatataa tattttaatt ataaagaaat atagaaatat
     9721 tttttaataa attagaaatt ggaggagatt acatgagaga ggcatcatat ttacaagaac
     9781 aactttataa acataggaag tcattaaatc ctattgcaga gattgggaga aaagagtata
     9841 agacttcaaa atatataaga gattatcttg ataaattagg aattgagtat gaagtttatt
     9901 tagaaacagg tatagtaggt gtaatagaag gtaaaagacc taaaaaagat attgctttta
     9961 gagctgacat agatggtctt catagtgaag aaggggtaaa acatttatgt ggtcatgatg
    10021 gacatgctac tatattactt ggattaattg agtatttaaa tgataataaa gaagacttaa
    10081 atgataatat agtatttata ttccaaccag cagaagaagg tcctggggga gcaaaagctt
    10141 taatagatga aggagtgctt agaaaataca atattgatga aatatatggg cttcatatat
    10201 atccagagtt accagaaggt tatgtaggta caagagaggg ctattttatg gctcaaatag
    10261 gagatattga catagaaata gtgtctaaaa gtggacatgg agctatgcca caaaatggta
    10321 ttgatggtat agtgatagct tcaaactttg taagctcact tcaaactata gtgtctagaa
    10381 atataagccc aatagacaat gcagtgttga ctattggtag aattgaaggg ggagctagaa
    10441 gaaatataat agctgaaaat ataaagatgg aaggtactat gagatgtttc agcccagaag
    10501 tgtatgagaa aatgaagctt agagttagag aacttgctag agggtttgaa ttggcttata
    10561 actgtaaggt taatataaat attatagatg attatattgc tgttaaaaat gacaaggatt
    10621 tgtatcaaga gtttgtagag gctattggaa gtgatactgt tgtagagtta gagccactta
    10681 tgatatcaga agatttttct tattatcaaa aagaagttcc aggattattt tttatgttgg
    10741 gttcaagaaa cgaagaaaaa ggatttgtaa atggtcttca taatataaac tttaattttg
    10801 atgaaaagat atgtgtaaat gcattgaatg tttattcaaa attattaaaa tacaaagaag
    10861 caatagatta ggaaaataaa ttataaagat taaaaagcat aaatacctaa atttaaaaaa
    10921 agtgtattaa taaatacccc ttacatattt gtataaattt attttaattg acaaaaatac
    10981 aaataagaag gggtgatttt tttgaaagaa aaagtacaaa gagaaaagaa gataactaat
    11041 ggcaagaata ctaaaatatt gacaaatgat gatataatga aatatgagat agcctcagaa
    11101 ctaggtctta tggataaagt tggtgaacgt ggatgggcag gacttacagc taaggaagca
    11161 ggaagaattg gaggtatgct tacatcacgc aaaaagaggc ttaaaaagat tgtggaggag
    11221 cagaataaaa aggatgagtc aatatagtga ttttgcgttt gtatatgatg aacttatgaa
    11281 tgaagttgat tataatggtt gggttaaata tatagaagat ataatagata gtgaaggtgt
    11341 aaaagtaaaa aatatattgg aattggcatg tggcacgggt aatcttacaa ttccattgac
    11401 taaaaaaaat tacgatatag ctggtataga tatatctgat gagatgctta gtgtagcaag
    11461 agaaaaggca gaaaaagaag gagtagaatt agttttactt caacaagata tatcagagct
    11521 tgattttgaa atatctgatt tggactgtgt actttgtgct tgtgatggat ttaattatat
    11581 aacttatgat gatgatttgg aaaatatatt ttcaaagaca tatgaattat taaagaaaga
    11641 tggaatattc atatttgata taagttcttt ttataaatta gccaacatat tgggaaataa
    11701 catgtatgga gaaaatagag aagatatagc gtatatgtgg caaaattact ttgatgaaga
    11761 agagaatcta attgaaatgg aacttacatt ctttattaaa gatgaatgtg gaaagtttga
    11821 aagatttgaa gagatacacc aacaaagagc atatactgaa gatgagatag ttgatatgct
    11881 tgaaaaatct ggatttaagg atataaaata ttatggggat tttacatttg acaaacctag
    11941 tgaagaaagt caaagaatct tctttgtatg taaaaaataa gatattcttg actatataag
    12001 ataattatta aaaataacca tgatataaaa atatagaaaa tgcagtttta tagattaaaa
    12061 gtaaagtgat gatactttat gacttttatt ttttaataat tataaagttc tatagataaa
    12121 aattaaaata tagtaactat tacttagtaa aattaaaata aaatgggttt tgcccaaggt
    12181 taagatagat aagtatttat agataaatta aggtagtaat ataataaaaa taagacaatt
    12241 aacgggacat ggaggaatat taatgagaga ttatgtacta agagcgacat caggaaatgg
    12301 tcaagttaga gcatttgtcg caactactag aaatacagta gaagaagcta gaagattaca
    12361 tgaaactaca aaagtagcaa cagcagcact tggaagaaca ttaacagcca catctataat
    12421 gggattaatg atgaaaaatg atagtgataa attgactgta ataataaaag gtggaggtcc
    12481 aattggaact attatcgcca catcagattc taagggaatg gtaaaaggat atgtaggaaa
    12541 tccacaagta gaggttgaag actatccaaa tggaaagtta aatgtagcag cagcagtagg
    12601 tacagaaggt gttgtaaaag ttataaaaga tttaggtctt agagaaccat ataatgggac
    12661 ttatccattg gtaagtggag aaattgcaga agactttact tattattttg ctgtatcaga
    12721 gcagactcca tcagtagtag ctttaggtgt tcttacaaaa gaagatgaag tagagtttgc
    12781 tggaggattt atagtgcaat taatgccaga tgcagaagaa gagactattg ctaagttaga
    12841 ggaaaatgta gctaaattac cttctataac taacatgtta aaagaaggaa aatcaccaga
    12901 ggatatatta aatattgttt tggatggttt ggaacctaaa atacttgata cctgtgaagt
    12961 tgggtttatg tgtgaatgtt caaaagaaag agtaaaaact gctttggttg caattggtaa
    13021 aaaatcattg gctcaaataa tagaagaaga taaaaaagca gaagttggtt gccagttctg
    13081 taataagaag tatatgtata gtgaagaaga gttgcttgaa attttaaagg aaatgtagac
    13141 tataaaaata attgtattga tataagtaat gttatagtga tatagtaaag tcgtattgat
    13201 atagtaatta aagtataatt tattaataat agacatattt atattaaaaa ataagtttaa
    13261 taaattttta agcttaataa attttaagaa ttaagaagct ttctggctgt aatgtttgca
    13321 atgttgcagt tagaaggctt ttttatttta ttatagttcg tgtcaaagat tgtatgaaac
    13381 aataaaatat tgtaaaataa tttgtatatt tagacctaat tgacacaaag gtttataaga
    13441 tatttgagtt gattatacga aattctatga agtagtaacc ctgtgacttt aatcatggga
    13501 ggttcagaag aaggagaaaa ttttaaatat ggaaagtaaa acgaagtaca taggaataag
    13561 tatattaata ttattgatga tatttttaat tgcaaagaat tttaacggaa aacttgaaat
    13621 acctattcct gagagtaata gtagaaaatt taaatcagca gcagtgtttt atcctcaaca
    13681 tcaagatgat gaggttttat ggggaggaag tgccattgta gatgctataa aacagtgtgg
    13741 tgttgataat gtatatgtgg tattagtatc agatggttct ggagttaacg ttttcaaagc
    13801 taacacaaaa tttagaaact taactaggaa gcaaaaggaa gagcttagaa ataacgagtt
    13861 taaatcagct ttgagagagc ttggagtgaa acctcaaaat gtaattatac ttgcagatat
    13921 agataaaaag gaaggcacac attatgaatt aatggaaaag actatattgg attttgaaca
    13981 taagtttaaa ggcaatgtga cccatatagc ccatcactat aagtatgatg accatataat
    14041 gcatagaaag aatggggaag ttttaaagaa acttaaacgt gaaggtaaga taaaagatga
    14101 cttgtatttt gttaagccaa aatatgtaaa gtatatacca agaaatgaaa gagctatcta
    14161 taaagcaatg gattttgatg actacaataa ggttaagagt gcttgttatg aatataaggt
    14221 tgtaaattca aagcgtaaaa tgtatggtat aggatatatt tcggcacata gttattttga
    14281 acatcttcta aatgaccctg aacttacgtc tgtattaagt gaagattaat ttcttataag
    14341 tagtaagtta tgtttgataa gttgattgga attttctata gttttgaagg tattggaaat
    14401 ttagttttat ttaaagattt aaagatagag gtttttaatg tttaaaattt taaagttaag
    14461 ttgagtaatt gtatagaatt atttgtgaaa gaagaaacag tgtaaaacat agtatcttat
    14521 ttatggtttt aaaatcatag gcaagatact ttttttatgt aactaatacc attttgagat
    14581 tattaactta agaattttat ataatttaac actcatatat aatgatgata tttaggtaga
    14641 atagatgtaa ttacgcttaa ataaaataac tgtatttttt ataaaaatca aaccctacac
    14701 tatactacca gataaaatct acatgctata atttgacctg tgtttataaa aaaaggagta
    14761 atctaataaa atagtatttc acttatgtat aactaacaac tattattacg attttctcta
    14821 aataatttaa agaaaaataa tgcaggaggt aaaatatgaa caatgaattt ataattttaa
    14881 aaggttgcaa agagaataat ttgaacaata tttccttaaa aataccaaaa agaaaaataa
    14941 ccatattcac aggggtgtcg ggttctggaa agtcatctat tgtatttgag acaattgcaa
    15001 aagaatcaca acgccaacta aatgaaaggt ttagtacatt tgtaagaagt tttttaccta
    15061 agtatggaga ggttaaagct gattgtatag aaaatctgtc aacaccaatt atcatagacc
    15121 aaagtagact tggtggaaat tcaaggtcaa ccctaggtac tattacagat gtaaattcct
    15181 ttttaagagc tttatattca aggtttggta gtgagtatat aggtaatgct aatatgtttt
    15241 cttttaataa tattgatggt atgtgcccac actgtcatgg gcttggaaaa aagctagttc
    15301 ctaatatgaa ccaaattctt gatatgaata aatcattaaa tgaaggtgct atattgcttt
    15361 cagggtttgg agtgggttct tggcattgga atatattcgc tgaatcagga ttttttgata
    15421 ttaataagag gatatgtgat tattcagaag tagaattgga aaagtttttg catggtaaag
    15481 cagaaaagat taagatagaa aatgctggtc aaactaacat gacatatgag ggattaatgg
    15541 ttaaatttaa tcgcctatac ttaagtagag agggagaaat atctgaagct actaagaaaa
    15601 aattgagtaa acttcttata gaagatagat gtcctatatg tgatggaaga agacttaatg
    15661 aaagagtata taggtcttta attaatggtt ataatatagc agatttaact agtatgcaaa
    15721 ttgatgaatt ggctgaagtt attaaaagta tagatgagcc agaagcacaa cctttaatta
    15781 aaggaataat agaaaagtta aacagtatca tagatatagg tcttggatat ttaacactag
    15841 atagagagac atcttctcta tcaggagggg aatctcagcg tataaagatg gttaagtatt
    15901 taaatagtaa tcttgttgat ttaatgtata tttttgatga accaagtgta ggacttcatc
    15961 caaaagatgt gtataaatta aataatcttt taaaaaaatt gcgtgataaa gggaatacaa
    16021 ttatagttgt tgaacatgac ccagatgtta ttaagattgc agaccatatt gtagatgtag
    16081 gaccaaaggc aggtaagtat ggtggagaag ttgtgtatga aggcagttat gaaaatctac
    16141 ttacaagtgg tactttgaca gggaatgctt taagtaaatt tcttcctatt aaagaaagtg
    16201 taagagaaca tagtggatat ttagaagtta aaaattgtaa taaaaacaat ttaaaaaatg
    16261 tatcgattaa gatacctaaa ggtgtattga ctctagttac tggagtagca ggagcaggta
    16321 aaagtacgtt gattaaggat gaatttttaa agcaaaatcc tagtgctgta cttatagacc
    16381 aaagccctgt ttcggcaaat tcaaggtcga gtttggcaac atatagtgga attatgaaca
    16441 acataagaag catattttca aaaacaaatg gcgtaaatgc ttctttattc agttcaaatt
    16501 cagaaggagc ttgtgataac tgtaagggtt ctggaatagt agaaatgaat cttgctttta
    16561 tggagagtat aaagagtacc tgcaatattt gtgaaggtaa aaaatttaaa aaagaagttt
    16621 tagaatatag gtttcaaggg aagaatataa ttgaagttct tgaaatgtct gttttagagg
    16681 caattgagtt ttttaattta aaacagatta aaacgaaatt acaatgtatt gaaaaaatgg
    16741 gtattggata ccttacttta ggacaaactc ttgatacttt gtctggagga gagtgtcaaa
    16801 gattaaagct tgctagtgaa ttaaataagg aaagttcagt gtatatatta gatgaaccaa
    16861 caacaggtct tcatatggct gatattgaga attttataag tataattgaa gagattgtag
    16921 ataatggaaa tacggtcata ataattgaac acaatataga tgtaattaaa agagctgatt
    16981 ggattattga gctgggacct gaaggaggaa taaaaggcgg gagagtaata tttgaaggta
    17041 ttccaaaaca attatgtaac aataaaatat cattaacagc aaaatatata gtataataaa
    17101 taaaatataa taaaattata atgatatttt aataaatata atgggtatat aaaatgataa
    17161 ttaaacttaa cagtaataaa taatgtgaga aatattcaaa tttaattaaa taaagtgtca
    17221 gaaatgtgtt taaattttag tattttaacg gttctgacct tttttcttat ttttatggag
    17281 aatatcgagg atggagtctc taatagttac ttttttgtgt ctacttgcaa gtttgattaa
    17341 aaaatggcta tacacacttg tgacttcagt catgggaggt tcagattata atcttaatat
    17401 ataatttgtg aaataatttg atgacgattt gctgatgtat aaacaataaa gattaactac
    17461 attaaaattt aaatcttgtt aagtaaagct tataaaagga gagagatgga tgataaatga
    17521 tattctaacc ttattatctg aaagaagtga tttttttata aagctatcca tagaacattt
    17581 gatgatttct attatttcta taataattgc gattgttttg ggtggtataa ttggtattat
    17641 tgtaagtgaa tttcataatt cttctaaacc tatattggga actatcaatt ttctatacac
    17701 cattccatct atttctatgc taggatttct gataccattt tccggaattg gaaatactac
    17761 tgctgttatt gcacttacca tttatgcatt attgccaatg gttagaaata catatactgg
    17821 aattactaat gtggataaaa atattcttga agcagctagg ggaatgggaa gtacagattt
    17881 tcagatattg tataaaataa aacttccgtt agccctacct gttattatgt ctggtattag
    17941 aaatatggtg attatgacaa ttgcattagc tggtatcgct tcatttatag gtgctggagg
    18001 cctaggtgta gccatatata gaggaatcac gacaaataac acaataatga caattactgg
    18061 aagtatactt attgctctat tagctttaat atttgatttt attttagggc tggtagaaaa
    18121 attttttcaa cgaaaaagaa aaaagaaaaa taggaaaaaa gtttttgcca ttctaggaag
    18181 ccttattctt atagtaacta tctttacgtc ttatttattt aatccttcaa agactcaaac
    18241 tattcatatt gctactaaac ctatgactga acaatatatt cttggtgaga tgttggatat
    18301 tttaattgaa aaaaacacag gtctaaacgt ggaaattaca caaggtgtag gagggggaac
    18361 atctaatatc cagccagcta tggagaatgg agaatttgac atttatccag agtatacagg
    18421 tacaggctgg aatatggtac ttaagaaaaa tgaattatat actgaaagta tgtttagtaa
    18481 actacagtcg gaatacaaaa ctaaacttaa aatgcaatgg actggtatgt atggtttttc
    18541 taatagctat gcattagcag ttagaaaaga agttgcagat aaatacaatt taaaaaatat
    18601 aacggactta aaagatgtat cttctaaatt gacatttgga gctgaatatg acttttatga
    18661 gagagaggat ggatatactc cattgtgtaa tacatatgga ctgaacttta aagatacagt
    18721 tgatttagat attggattaa aataccaagc tatcaatgaa ggtaagattg acgtaatgcc
    18781 tatatttaca actgatggtc agtatagtgt ttcagatgtt gtagttctaa aagatgataa
    18841 aaatttctac ccatcttatc aatgtggaaa tgttgttcgt ttggaagtat tagaggaaaa
    18901 tccagaacta tcaaaggtat ttaagatggt tggagatatt ttgactgata aagaaatggc
    18961 taaaatgaat tatgacgtag aaaacaacaa tttagaacct agagaagttg ctcgtgagtt
    19021 tctgaataag aaaggtcttt tataaattgg aggaaatata atgacaccta ttatacaatt
    19081 taaaaatatt aaaaaacaat ataatgataa aactataata gataatctta atttagatat
    19141 agaaaaagga gagtttttga ctgtaattgg ttcgtctggt tctggtaaga caacactctt
    19201 aaaaatgatt aatggtctga ttttgccaga tggaggaaat attttgataa accaaacaga
    19261 tatcaaaaac gaggacttaa ttaaattaag aagaagaata ggatattgtg tacagggaag
    19321 tgttcttttt cctcatatga ctgttgaaga aaatatttca tatgtaccaa atctcctaag
    19381 taaaaagaat aaattagaag taaaaagtgc tgtaaacaag tggatggaaa tagtgggatt
    19441 gccaaatgat atgaaagtgc gttacccatc agaattgtca ggtggtcagc aacaacgtgt
    19501 agggattgca agagctttag cttcttctcc agaaatttta ttaatggatg aaccttttgg
    19561 tgcagttgat gagattacac gaaagcaatt acaaaaagag ataaaagaaa ttcataaaaa
    19621 aacaggagtt acgattatct ttattacaca tgatatttat gaagctttga ttttgggaac
    19681 aaagactttg gtattaaatc atggagttgt tcaacagtat gataatcctg agaatatttt
    19741 aaatactcct gctaaccaat ttgtagacca gttattgaat ataagaaaat ctataataga
    19801 tgatgataat ttaaaagaat aaatttaaat ttaaaatcaa gctgattttc aatagttgtc
    19861 tgaaaagaag atggctttat agtatctcac cccctttctt gccatgaaag agattggatt
    19921 gaagttattt gctccctttc gtactaagtc tcgacactaa gggcctacca gttgtattga
    19981 tggcaaaaaa ctatgagaaa aatatccaac aacttaaaag tcacacaaac agactttgca
    20041 atctgtttat gtgctttatt aaatgtgtct gttgatgagt ttttcttacc tgtcgctata
    20101 tggtaaagag cattagacgc aggcaggtat aaaacatagg gaaagtttta cggagaaaga
    20161 gttaatgctt atggaagctc tggcaaatgg tataaataaa acgaaagaag cagaggaata
    20221 accaaatgtt ccctgctttt cttactttga ctcctaatta caaacttgtt tcatactgac
    20281 aaatgaagta tttgatatat tttatttttt aatgattttt ctggttctct ctcataaaag
    20341 tgatactcta catatttcca tttatcggga ttatatatta ggactttgcc taaacatcta
    20401 tcagaagttt tcaagaaact taatggtgac atttgattcg ttggtaaaat atcattttca
    20461 atttccatag catattgaga tttattaaat tccttaccta aatcacataa aagcgggata
    20521 ccaatattta ttttctgcca accaaaggat tgcaaaatat tgttatagaa tccatcaaaa
    20581 ataaatttat tcatcccgcc actatttttc cataggtaca aaggtgaata ttcgttatgg
    20641 ctttcagata taccattctt ttccttaatt aaatatgctt taaacattaa atcatcaaag
    20701 ccgtctgtct tatatccatt attttttaca cgttgtctta tgttatccat atcataatca
    20761 tctggtaaaa gaattttata ttgcattgca atcattttta ttcctcctca attcggttaa
    20821 caccctaata agtatctttt tacttacact tttattatac atttgttctt gtatcatgta
    20881 taatagttgt taatgatggt tgatatcatt tttagtgatg gaggatatgt tatggaaagt
    20941 ttagaattaa gaattttcag agaagttgca cgtactaaaa atatatcgag agctgctaaa
    21001 aatatgggtt atgtgcaatc caacatcaca gctcacataa aaaagttgga aaatgaatta
    21061 aataccactt tatttatacg gtataataaa ggtgtaacgc tgacagaaga tggagaaaag
    21121 ttattgcaat acgctgaaca gattatatca ttactggata aagctttata tacatttcat
    21181 agagatatta gacaattaaa aatcggagcg actcaaacaa tagctggata tttattacct
    21241 caatgtttga ctgaatacca aaacagattt cctaatatca atatttcgat aaagacctct
    21301 aaccagcaag atatggataa acagttaaga cagaaattac tagactgtgt tatcacgaat
    21361 gaccctcatg ttttcactga tgctcaatgt atttttaaga ctagagaatt tctttctatt
    21421 atagcgccta aattttgtga atctatttcc caaatatggc agttaccaat agttgttaac
    21481 aacttaaaga catgcccata tcgcaagata gtaacggatt tgtttttacg tgaatctgca
    21541 tcattcaaaa taatagagct tgatacagtg gaatctatta tcaacacggt tgctgctggt
    21601 gggggaattt cattacttcc caaaaatata ttacgaaata aattaactat aaatgagttc
    21661 gatattccag agctagagcc tacctcaatc aatatatgga ttcataaaga ttctttcccg
    21721 tcagatttta taacatttaa agaaattata gaaagactaa cttgttataa ataaatgatt
    21781 ttttgacacc cagtaagccg ataaaagcag cagcgagggc gtttgtcaca aagtattttc
    21841 atttaattaa agagggcagg tttcagataa actatatgaa tttgtaaaat acggagaaaa
    21901 aacggatgag aatagtttga gtgaagttta gtttttggtc ccaaattttt aaaagaaggt
    21961 atcatgtata aagtagatgt atttagatag gaagagtatt ataatggtat gtatttataa
    22021 ataatttata gtctgtagga aagaaatagt tttactataa gattatgata aaatacacaa
    22081 tactatgata aaatagtact aaataataga tttaattttg caaaggataa ggtgttttaa
    22141 taaaaatgaa tgtagaaaac gaattactta aaaacctaga tagattgcat acaacagagt
    22201 taggcgttgt aagaattaag aaaaatcttt ctttggagac aaatgatgta gtgaactggt
    22261 gtaaaactaa aatagaatct ccaaatgcca ttattaatag aaagggaaaa aactggtata
    22321 taagtgtata tgattgtatt ataacagtaa acgctcacag ttatacaatt attacagctc
    22381 ataaagaaaa gaaatgaatt gcaaaataca aaggtcaaaa gcattttcca ctgtttgtag
    22441 tacctatgaa gaagtatata aaataatatt acttttgaat aataatttaa attttggagg
    22501 ttgaaaaaat gaatgatact gtaaaagaaa aattggcaga gtcactaact tctgatacta
    22561 ctgatatttt accttatttg ccttatttat tacaagattt ttgggaatta ggaagttcac
    22621 cagaacaaat gttacaatta tttagaaagc atataccttt taatgaagat acaaaagttt
    22681 tggatttagc ttgtggaaaa ggtgctgtat ctgtgaagct tgcaaaagag ttgaatatac
    22741 atatcaaagg tattgatttg atacctgaat ttattgatta tgcgagaaga aaggctattg
    22801 aatataaggt tgatgctttg tgtgattttg aaatgaatga tgctaatagt tctgtaaaaa
    22861 ttgagcatga ttatgattgt gtgatttttg gagctgtagg ggatatttta ggtaattata
    22921 aagaaactat aagtaagcta ttacctacaa taaaaagtaa ggggttcatt ttaattgatg
    22981 atgcatatgt agtagataaa tctacaaatg aacagcttaa ttttgagagg gtgtatccca
    23041 cttacaatga gtggtgtata ttgttcaaag aattaggtct gaagctaatt gaatgtatac
    23101 catgtttaga acttgaaaat gatgttgaag aggaaatgaa ttggattata actcgtgcaa
    23161 atgagctaat taaaaaatat ccttctcaaa aagatatgtt tgaacaatat gttattagcc
    23221 aaaaaaatga atatactgat ttacagcagg accttgttgg agcaacttgg attttacaaa
    23281 aagaataaga taaatattag actagataat caatgttata gtgaattata ccatgtctag
    23341 tgaatataaa taacataaaa aaccgtcaag atgaatagtt tattcgtgct tgacggtttt
    23401 tagtatttgt tgaagtaaaa ttttatttct ttagtatatc acttttagtc aatccatttg
    23461 atttagcctg agtattccat tgatgattcc ccataagttt atataggaat ctagggaagt
    23521 taggtgaaat aaaaatagta cttttgttgg aatcatctat atgtatatta tcactaagta
    23581 tattttttga aagttctttt aatgcaattc ctaaatcttt tttaggtcct ttacccaaag
    23641 gtacggattc tagtgaacca aacatctccc cagctccaat tccaatacct tgaacccatg
    23701 taaagttgat ttttttacac caatgctcta tcatttgaat tgccaaatga ttatgttcac
    23761 cttcattgaa tccacagtta actattgcat acactggaat aggtttttta ttttgtgttt
    23821 ttaaaaattt ttcacatttc tctaaaaaat gaagtaaatg agatggaata ccatctacat
    23881 aaagaggaaa tgctattatt aatatatcac aattaagtat ggcattaaga gcgtcaggat
    23941 acttagatgt gctatctact ttaaatgtaa aaactttatt atcattttga ataagttgtt
    24001 ttaattcaga taaaaaatac tgtgaattat ttttatttac ttttggacta ccatttatca
    24061 ttactatatt catatggatt cacctcttat atcctgtata ttttcataga aacaaacaga
    24121 gtgttctttt atattgaaat ttatagcatt tccatttact aatttcgtag cagtttcttt
    24181 ttcagcttct gtaatacaat ctccatagaa tatagctttt aaatttatct gtttatcata
    24241 tctgctttta tgacgcatca tattatcttt tattacaaag tatggaagaa tataattaat
    24301 gcttttatca agtacgtttt tgacaaaagg gctaaaccct ccgtaacagc atctgcttat
    24361 tataataatt ccatcagagt catttataag tttacccatg ttgctgtaat aatcttttac
    24421 aacacattca gaaggagttt taatccagca accaaaacaa ccaatacatt tgtggatttc
    24481 ttttgtgttt ggaagtattt ttatattgtc atcaagtttg ggtaataact tttctaattc
    24541 tttgtcagtt aaatcatgta ttaaaagcct cataaagtcc atctcctttt attatgtatg
    24601 tttacttcta aaaattatat cttatagatt tttcagtaaa tctttacacc aattaataac
    24661 aaaattataa tagtcaactc cataaatcat tgttgcatac atatatgtat attggtcaac
    24721 acaatgattt tcttctgcat catttttttg tttacttaat ttatttagaa gtaattctac
    24781 ttcagttatt aacattttta agtttgtttc aattatttct ttgggtagat acttgtaaaa
    24841 gaatatatta acaaggtggt cttgctttgt tttagaaaaa tttataggtt tctttaacca
    24901 ttcaagaaat acttctttac cagtattagt tatggaatat agttttttta atttgctacc
    24961 atctataact tcgtgatagt gtatatatcc ttttgtttct aatcttttaa gtgcaggata
    25021 gatacttcca aagctggcgt caaaaaagta cgaggtactt tctgacatta tttgttttaa
    25081 gtcgtatcca cttaattctt tttccattaa aaatcctaaa ataatgtatt ctaacatatt
    25141 acctcctggt atcaattaga tatatatcga ttcgatatat ttattattac agataataaa
    25201 tcttacgtca agatgctttt taaaataagc taaaaagaat taggagttat tgataaatat
    25261 gtaaaaaaat taatataaaa ggtaattaaa tatcaatgaa gatttaaaaa tagttaaatc
    25321 aaagtaagag tctaatatat aaaatttata gtaactttat ggtttaaata taaacttgtg
    25381 tgctattgac aaagtataaa attagcagta gaataattgc atgagcaaat agattgttgc
    25441 gaacgcaaaa aaaggacaat agatattaaa ggagagaata tgagagagct attaaaatgg
    25501 gtttccatta taaatagata caatacaaat tatattgaca ggaaattggc ggatattgga
    25561 atcaataata gccaatattt ttatgtatta cacatatgta ataatccagg tattacacag
    25621 gacactatat ttgagaaaat tcttgtgaat cctagtaata taacaagagc tttggcaaat
    25681 ttagaaaaag aaggctttat aacaagagaa ccaggtgtaa aggataaaag aacatggcat
    25741 ttatatccta cagaaaaatc tttagaatgt tatgatgaaa tactaaaaat aacaaatggg
    25801 tatgttgagg agttgttgag ttcatttgag aaagaagagc aagagttgtt tatgtcaatg
    25861 ttaaagaaag ttgcattttt ggctattgat atgaatgaag aagcaaaatt ggaggcgaag
    25921 gaaaatggaa gaaaaatata aggagccatt ggaatatgaa aaggtttcaa agttattaac
    25981 taagtacgca attcctagta ttatagcaat gttagtaagt gctttatata acattgttga
    26041 ccaaatattt attgggcaag gtgttggtgt attgggtaat gctgctacaa atgtcacttt
    26101 tccactaaca acaatatgta ctgcaattgc attattgtta ggaattggag gagcgtctaa
    26161 gtgttcattg gaattaggtg ctaaaaattc agaaaaagct acaaaagcag caggaaattc
    26221 tatatgcttg atggctgttt ttggaataag tttatttata gtagtatcaa tatttttaac
    26281 accactatta aaattctttg gctctacatc agaaatacta ccttatgcac acacatatac
    26341 aaaaattaca tcaattggat taccatttct tataatgtcc actggaatga gtaagttgat
    26401 acttgcagat gggagaccaa aagcttctat gttttgtatg ttaataggag cagttattaa
    26461 tacaattttg aatccattat tcatttttgt ttttaatatg ggaattgctg ggtctgcttt
    26521 agcaactgtt attgggcaac ttatatcttt tggaataagt gttcattata taaagaattt
    26581 tcaacatatt aaattacata agaaaagttt taaattagat agtataattt ataaacatat
    26641 atttgcgcta ggttcaagct catgttttaa tcaattagca atgactgtaa cacagatagt
    26701 tatgaataat acattggcat attatggggc taggtcagtt tatggaagtg agattccact
    26761 tgcttgtgtt ggtatcatta ttaaagtaaa tatgattttt atatctataa ttattggtat
    26821 atcacaaggt atgcaaccaa ttatagggta taattatggg gctgaaaagt ataatcgtgt
    26881 aaaagaagca tataagtttg cagtgggaag tgcaactatt atatctgtta ttgcattcct
    26941 atgtttccaa ttcttcccaa gacaaattac gtctatattt ggtaacggaa atgagactta
    27001 ttttatattt gcagaacatt attttagaac tttcttgttc ttaacattta taaatggtgt
    27061 tcaaatagta tcatcaactt tctttacatc aattgggaaa tcagtgttgg gactttttac
    27121 ttctttgact cgtcaaattt tatttttaat tccattgata gtaattttac caatgtttat
    27181 gggaattgat ggagtaatgt ttgcaggacc aatagcagat gcagcagcgg caatggtatg
    27241 tatatacttt gcagttcatg aaatgaaatc attgactata aagcaaaata atttgatgag
    27301 taaccatagt aagattgcag aggtataaga caatatttaa gaagtataga aaattaattt
    27361 aaaaaatata ataaattaat ttaggaagtg tagaaaatta atctaaagaa gtataataaa
    27421 ctaatttaga aagtatagaa aattaattta aagaagtgta ataaactaat ttaggaagta
    27481 tagaaagcta atctaaagaa gtataataaa ctaatttagg aagtatagaa aattaattta
    27541 aagaagtata ataaactaat ttaggaagta tagaaaatta atttagagaa gtataataaa
    27601 ctaatttaag aaatatagaa aattaattta aagaaataca atacattaat ttttaagtaa
    27661 cattaaatta atgtaagtaa aataatgttc atatgaagat ttaaaggtgc attttctgaa
    27721 gctaagtata tgttgtagag tgttacgtct ctctatcctg tgaagtttaa tagctcaaga
    27781 aaatgcacta tttatgatga ttattttgtt ttatttccaa ttttggaaat aaatattcta
    27841 attaaatcta aaattatacc ataaataaga aaagaaagta tagatgctat gtaccaataa
    27901 atcgaaattg accctgtaat tgttctacat gggatttgca tacctgaatc cataaaaaca
    27961 aaaaaactat caactctgga aataatagac aaaataggat ttaaacctat tagaacaata
    28021 ctttttgagt cttgaccaat ttgatgcata taaatctcaa ataatgcaat taaaaaaaac
    28081 caaaatgtaa aagatttaat aatttttttc atcaaaatac ctcacataat ataaattctg
    28141 tttgcactat ttgcttacta caactattta tctccattca gtcacatcac ttaattgtaa
    28201 tctaggtctt cttgatggtt cttcatcagg ataacccaaa gatattgctg ccactaattg
    28261 actatctgta tttaaccatt cacataattc acgatatgca aagtatacat cacaaatcca
    28321 aagactacct agacctaatt ccacagctgt aagcgacata ttctgaattg cagctccaat
    28381 tgattgcatg tttgccattt catagaatcg ttcttcaggg gatagctttt ctaaaggtga
    28441 tttacctaat atatttaaaa taaaaattgt tactggagct tgtttcatga tttcaactgt
    28501 gtaatttgca ccagcgatat gttgacgact tcctggaaga agcccattat cattaatttc
    28561 attttgaata cctttgctca tggctttaag catagattct ttttcctttt cagtgatgac
    28621 aacaaaacgc catggttgcc tgtttttaga tgaaggtgca tttattccag cttcaattat
    28681 tttctctata gtttcatgtg aaatgctttg atttttatat tttctgatac ttcgtctttt
    28741 tgatatagaa tcacttatca ttttaagtaa cctccgttta ttttttgaaa aaatctattt
    28801 aatttttatt ttctatttta attattttag cattaatcag aaaaaaagtc attgatttta
    28861 gtatacaatt attaatgatt atattaagaa ttgtatcaaa aagtatgt
//
LOCUS       DAEMHW010000034        28274 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEMHW010000034 DAEMHW010000000
VERSION     DAEMHW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.034"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..162)
                     /locus_tag="KPK67_001270"
     CDS             complement(<1..162)
                     /locus_tag="KPK67_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458263.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVH"
     gene            complement(164..1096)
                     /locus_tag="KPK67_001271"
     CDS             complement(164..1096)
                     /locus_tag="KPK67_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG2458264.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(1141..2073)
                     /locus_tag="KPK67_001272"
     CDS             complement(1141..2073)
                     /locus_tag="KPK67_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG2458265.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(2128..3417)
                     /locus_tag="KPK67_001273"
     CDS             complement(2128..3417)
                     /locus_tag="KPK67_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2458266.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(3446..4138)
                     /locus_tag="KPK67_001274"
     CDS             complement(3446..4138)
                     /locus_tag="KPK67_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2458267.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(4153..4914)
                     /gene="pgeF"
                     /locus_tag="KPK67_001275"
     CDS             complement(4153..4914)
                     /gene="pgeF"
                     /locus_tag="KPK67_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBG2458268.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDAIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(4911..5372)
                     /gene="nrdR"
                     /locus_tag="KPK67_001276"
     CDS             complement(4911..5372)
                     /gene="nrdR"
                     /locus_tag="KPK67_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBG2458269.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(5394..5657)
                     /locus_tag="KPK67_001277"
     CDS             complement(5394..5657)
                     /locus_tag="KPK67_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG2458270.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(5761..6534)
                     /gene="sigG"
                     /locus_tag="KPK67_001278"
     CDS             complement(5761..6534)
                     /gene="sigG"
                     /locus_tag="KPK67_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBG2458271.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(6626..7348)
                     /gene="sigE"
                     /locus_tag="KPK67_001279"
     CDS             complement(6626..7348)
                     /gene="sigE"
                     /locus_tag="KPK67_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBG2458272.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(7373..8245)
                     /locus_tag="KPK67_001280"
     CDS             complement(7373..8245)
                     /locus_tag="KPK67_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBG2458273.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYIHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(8535..9809)
                     /locus_tag="KPK67_001281"
     CDS             complement(8535..9809)
                     /locus_tag="KPK67_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG2458274.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKNSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(10093..11250)
                     /gene="ftsZ"
                     /locus_tag="KPK67_001282"
     CDS             complement(10093..11250)
                     /gene="ftsZ"
                     /locus_tag="KPK67_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBG2458275.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(11440..11793)
                     /locus_tag="KPK67_001283"
     CDS             complement(11440..11793)
                     /locus_tag="KPK67_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBG2458276.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(11892..12566)
                     /locus_tag="KPK67_001284"
     CDS             complement(11892..12566)
                     /locus_tag="KPK67_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG2458277.1"
                     /translation="MQKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(12571..13269)
                     /locus_tag="KPK67_001285"
     CDS             complement(12571..13269)
                     /locus_tag="KPK67_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG2458278.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(13292..14032)
                     /locus_tag="KPK67_001286"
     CDS             complement(13292..14032)
                     /locus_tag="KPK67_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBG2458279.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(14302..15528)
                     /gene="murG"
                     /locus_tag="KPK67_001287"
     CDS             complement(14302..15528)
                     /gene="murG"
                     /locus_tag="KPK67_001287"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG2458280.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(15546..16676)
                     /gene="spoVE"
                     /locus_tag="KPK67_001288"
     CDS             complement(15546..16676)
                     /gene="spoVE"
                     /locus_tag="KPK67_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBG2458281.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(16728..18083)
                     /locus_tag="KPK67_001289"
     CDS             complement(16728..18083)
                     /locus_tag="KPK67_001289"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBG2458282.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(18102..19070)
                     /locus_tag="KPK67_001290"
     CDS             complement(18102..19070)
                     /locus_tag="KPK67_001290"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBG2458283.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(19111..20484)
                     /locus_tag="KPK67_001291"
     CDS             complement(19111..20484)
                     /locus_tag="KPK67_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBG2458284.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(20651..22630)
                     /locus_tag="KPK67_001292"
     CDS             complement(20651..22630)
                     /locus_tag="KPK67_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBG2458285.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILGE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(22642..22989)
                     /locus_tag="KPK67_001293"
     CDS             complement(22642..22989)
                     /locus_tag="KPK67_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458286.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(23470..24405)
                     /gene="rsmH"
                     /locus_tag="KPK67_001294"
     CDS             complement(23470..24405)
                     /gene="rsmH"
                     /locus_tag="KPK67_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBG2458287.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(24435..25181)
                     /locus_tag="KPK67_001295"
     CDS             complement(24435..25181)
                     /locus_tag="KPK67_001295"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBG2458288.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(25575..26861)
                     /locus_tag="KPK67_001296"
     CDS             complement(25575..26861)
                     /locus_tag="KPK67_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG2458289.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(26876..28141)
                     /locus_tag="KPK67_001297"
     CDS             complement(26876..28141)
                     /locus_tag="KPK67_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG2458290.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
BASE COUNT         8646 a         5322 c         2817 g        11489 t
ORIGIN      
        1 atgaaccatt ccacctacac actttgacat caacaatgcc ttattttgta agtctttctc
       61 atcatttgca taaaatattg gacaacctcc accatttttc atattcttat caagtgtaac
      121 tatagctagg gtatattcat ttaaacctat atccattccc atcttatgca cttcctttct
      181 ttcctatttt ataatttttt agtgataagt tttttccttt agagcttgaa agtattgggc
      241 atgactttac agcttccatt actttttcca cattcttatc tactggtaaa taagcaatca
      301 ttatactttc atcctcttta tttcttctag gtaatggtac gaatgaaggt tctccataat
      361 ctccatataa acctaacctt gaatatatat tgtatactat tgcttgtctt tgaccggagt
      421 caaataatat tcctgcatta gcataatcct tatttttagg aattatttgt attcctacac
      481 ctctattaat atatacttct ctatcgcttt ttagacctat atttgttata ccttttaaat
      541 ctcccacctt tagtatagat tcgtccacaa aactaatttc agcaggcatt acatcagcta
      601 tgtctccaat actttttcta gtaagaagtt tttttaatat aaaggctaac actaatccac
      661 aaattgaact tattaccaaa ctcatccctt cacttatttt cgtttctgca agtattacat
      721 aatacaatcc tattgttaaa aaagatgtaa ctatacacat ataatttctt acttcatagg
      781 ttctagctat ctcttctatg aatgcagctc ctctctttac aagttgaact tcctctaagt
      841 tttgcaatgt atttcttcta tttgctctca cttgtctaaa ttgctctgct gctagggata
      901 aaaaagttat agatgtataa gaacgattta taagagctgg aaccaataat gctcctaatg
      961 aagaagcgac gaatgctaat ataagttgag atagtaaaac atttggttgc gttggatact
     1021 gtttttggtc taaattcaac attataattc tagatgttat accaataata acagcaacaa
     1081 aaaatactct atccattaaa tcactcctaa aatatttaat ttgaaatttt acatgtaaat
     1141 ctacaaagtc tttctagaaa aatcactctg ctttcctttt gatgtttcta tgataggtgt
     1201 acttttcact acatctatca tgacatctat atctttgagt attggcatat atggtataac
     1261 cacagtactt ttttctaaat tggtcttaga tatagcaaca atatcaacct catctacatc
     1321 tctatctatc cccatatgaa taaatagatt atgtattata gcttgtcttt gccctatatc
     1381 atttactatt ccaaatgcac ctaaatctct tggtataact tctatggcca gacattcatt
     1441 tctatacttt tctcttgtac tttctagacc tatattagtt attattacac catttaccat
     1501 taaaataggc ccttcaaaac ttatttttgc aggaactata tctgctatat ctgctatact
     1561 gttccttctt aaaaatcttc taaaaatcaa acctacaata gctcctgaca caatagctaa
     1621 gattgtgcaa taaaaaaagc taaggccata ctttcttgca aaaacgatat aaactatcga
     1681 agataccagt gcagaaaaaa gacttatata acttctagat tcatacgtag atgctatttc
     1741 ttcaacgtaa gcatctcctt ttggtactaa ttcttcatta tcgatattct ttagtgttat
     1801 tctttcttgt tctgcaagac cttgaaactg ttgtattgct actgcaaaaa atgtcaaggc
     1861 tgcaaactcc ttatctataa gtgcaggtaa tgctatagca cctaacgaag ctgaaagccc
     1921 agatataatt atctgttcta aataatcttg tggtcttgat gggtattgtt tgtctgtaac
     1981 tcttaaaact aatcctctac ataatattcc tacaactaat gcaactataa aagagtgtct
     2041 aaataaattt gcattcaata aactttcttc cattttctca gctccttctc ttatatagtt
     2101 tgtgcaaaaa acaaaaaaaa ttcccttcta tttaaggaaa tttttttgta atattgtaac
     2161 tataaattcg ctaccaatgc caagttcact ttttactttt atacatccat ttagtgattt
     2221 gactatatgc tttgttatag ccaatcctaa acccgttcct cctacatccc tacttcttgc
     2281 tttatctacc ctataaaatc gttcaaatat gcgttccaca tcttcttttg gtatccctac
     2341 accattgtct ataactttaa ttacgatttt ttcttcatct tttaacacta caacttgtac
     2401 aatcccatta tcttgggtat actttatagc attatcaact aggtttaaga acacttgctt
     2461 tatatagtct ttattggata gtatacttat tgatttatca tcacatttat ataaaacttc
     2521 tatatttttt tcactagctg aatgttgagt tatttcatat acctctttaa atacatcata
     2581 tactacaaca ttttccataa gaagagtatc tttattttct acaaatgata acagcaatat
     2641 atcatcaatc aaccttttta atctatctga ttcgctttct attatttcta agaatctatt
     2701 tctagtattc acatctatat tttcatttga ctttagtgtt tcaacaaacc cacttataga
     2761 tgtaagtggt gttttaagtt catgacttac atttgcaaca aaatcacttc tcatattttc
     2821 aagtttaact tttttagtaa tatcttctat atttactatc gaacctatta ttacgttgtt
     2881 agtattctga agataaactg gatctacttt tatcttatat acgatatcat ctttagtagt
     2941 aatattagca gtttcattat ctttagaacc aataaattga agtatttgtt ttaatatttc
     3001 ttcttctttt attacaacat taatattatg accttcaact aattgatttg gttcactctt
     3061 tatcatttct tttgcttcat catttatcag aagtatattt ccatttacat ctatagccaa
     3121 aattccatga gatatactct ttaatataga ccttaactgt aaatgtttat actgtacttc
     3181 ttctaaggtt ttatccatat tctttatcat atgattaaaa tttctagcta attcgccaag
     3241 ttctcctttg gctgatatgt taagcctagc atgatattca ttattactta ctttttttga
     3301 gacgtatata aactctttta aatatttcct taatgttatt gtgtatctta cagctaatat
     3361 aacaatggct atggacaaca taacaataaa aaaatatata ctatttaaat cttccacaag
     3421 ataacactcc taaaatataa cctttttaat ctattttgta tcctacacct cttatagtct
     3481 ctatatattt ttccgattta tcttcatctt ctattttttt tcttaaatat cttatatgaa
     3541 catctactgt tctagtttct ccatagtatt cataccccca tattttatcc aaaagataat
     3601 ttcttgaaag tacttttcct ttattttgaa ttaataattt taacaattca aattccttaa
     3661 gagttaactc tatcttttca gagcctttgg taacttcatg ttttgataaa tctagtttta
     3721 aattacctgt agtaagtata ttgttttctt cacctaagct agtcaaatta tatctcctta
     3781 aaacagaact aatcctagct aacaactctt ttatactaaa tggcttagtt atatagtcat
     3841 cagcacctat ttcaagccct tcaaccttgt cattttccat atttttagca gtaagcatta
     3901 taacaggaat attttttaag tccttatcac ttcttatttt tctcaaaact tctattccac
     3961 ttatatttgg taacatccaa tctaaaagta ttaaatctgg tttaatttcc ttagccttta
     4021 taaatccgtc aaatccatca tatgaataac taacttcgta atttgacacc tccaaattga
     4081 attttaatag ctcaactata tgcatttcat cgtctataac aagcacttta gtattcatta
     4141 aaaagctcct ctctatttta tctgtagtac cgttcctatt cgttctgatg ccttattatg
     4201 ttttctataa gagtggaatt tatctgctct acaactagtg cataaattta aatttattat
     4261 attttctttt ttaactccac tacatctaag catatactca ttaattttcc ataaatctaa
     4321 atagtaactt ccctctttta ttatatagaa cttttcatca ttatttgtaa gaattgtgtt
     4381 aaatttttct actaaatctt cagacacttc ataacaacat ggtccaattg atggcccaat
     4441 tatacatact aaatcttcag gaactgtttt atagcattca gacattaatt ttattgtttt
     4501 atgacctata ttactaaaag tccctctcca acctgcatga gccagtccta tggctttatt
     4561 tttagcatct ataattgata taggtacaca gtccgctgta aacagtaaaa gtggtacttt
     4621 ttccaagttt gttataagtg catctccatc tcttcttttc cctatagcat ctttattgac
     4681 tatattaact atatctgaat gtatttgaga attactagtt agattttcaa atgtcaaatc
     4741 acattcttta catactttta acatatcttc tctatttttt gcatctatat tcttacatgt
     4801 tataactaga tttacagaat ttaatttttc atcaagttct tcacaattac cacttaaatt
     4861 aacatactct ttaatatctt gtacattttt tatatttata taatctttca tcatttgtct
     4921 cctttttcta tcagtatact tttaagttca tttacaaaag tatttatatc tttaaattgt
     4981 ctatacacag aagcaaatct tacataggca acctcatcta actctttcaa tttatccatt
     5041 accatctcac cgattttttt ggtttcaatt tcttctataa aacatttatt tatttcattt
     5101 tcaatatgta caacaatact ttcaatctcg tcaatagaaa caggtctttt ttcacatgct
     5161 tttagcaatc catattttat cttttctcta tcaaaatatt ctctactatt atccttttta
     5221 atgaccatta aaggagttgt ttctattttc tcatacgtag taaacctctt tttacatgat
     5281 tcacactcac gtcttcttct gatagatttt aaatctgtat gtctagaatc tattaccttt
     5341 gattctttat aattacaata aggacattgc atctatattc ctcctttgtt taactatatc
     5401 tcaaactcat ctaagcttaa ttctgaacct atatttacta ttattgtatc acaacctatt
     5461 ttaagtatat cattccaaaa tataacttct tcatcccctc ctcttgaaaa aaaacttcta
     5521 cttccatctc caaaaatggt aaatgagact accttacctt cgtgaatatc catgtctatg
     5581 tctattataa accccatcct ttttccattt tttacattta taatttcttt ttccattata
     5641 tcagaaactc taatcatatt gtactcaccc tttctttaaa aataaatatg tatcgaagta
     5701 aaaaaaaatg acctatatag gtcatttaaa ttaaaataat gtttacagtc catggattac
     5761 ttatacatat tttctcatat tctttaaagc atttttttca attcttgata cttgtgcttg
     5821 agatataccg atttcatcag caacttctat ctgagttctt cctttgtaaa accttaaatc
     5881 taataccaac ttttctctac tatttaattt cttaatagct tctttaagtg atatttcttg
     5941 caaccaattt tcatcagtat cttttttatc ttgaacctga tccataacga atattgcatc
     6001 tccattgtct tgatatactg ggtcaaacag agatattggg tcttgtatgg catcaagtgc
     6061 cattacaact gattctactt ctaattctaa ttcctttgct atttctgata ctgtaggttc
     6121 tttagaattt gttctaatta atctttctct aacttgtagt gccttatatg ctatatcttt
     6181 taatgaccta cttactctaa ttgggttatt gtctcttaga tatcttctaa tttctcctat
     6241 aatcatagga acagcataag ttgaaaacct tacattttgg cttaaatcaa agttatctat
     6301 agcttttata agaccaatac aacctatttg aaataaatcg tctatatttt ctcctctgtt
     6361 gttaaacttt tttattacac tcaagactaa tcttaaattc cctcttacaa actgttgtct
     6421 ggcttcttca tcaccatttt ttatttgtaa taaaagttcc ttcatttgct tatttttaag
     6481 aactggaagt tctgatgtat taacaccaca tatttcaacc ttattaactt gcatatattt
     6541 tcagtcctct ctcaaaagta ttcatctatt gaaattattt acatttccaa tttttttata
     6601 ctttttaaga agaattaatt tagcattata caaatttttt catttctttt tgcaaccttg
     6661 agattatttt cttttcaagt ctagatatgt aggactgtga tattccaagc attccagcaa
     6721 cttctttttg tgttttttca atacctttat caataagacc aaatcttaat tccataattt
     6781 gcttttctct gtcacttaat ctgtcaagtg ccataaccaa caaatctcta tctatttctt
     6841 cttcaattat tttgtatatt tcatcatttt ctgtacccaa tacatcagat aataaaagtt
     6901 cgtttccatc taaatctata tttaaaggtt catcaaatga tacttctgtt ttctttttat
     6961 tattttttct taagtacatt aatatttcat tttctataca tctcgaagca taagttgcca
     7021 attttatatt tttattcagt ttaaaagtat taactgcttt tatcaaacct atagttccta
     7081 ttgaaatcaa atcttctaca tctatacctg tattttcaaa ttttctcgat atgtagacta
     7141 ctaatctaag atttctttct attaaaattg actttacact ttcatctgtt tctagctttt
     7201 gtaaaagctc catttcttct tctggtttca atggaggagg aagtatattt gcccctccca
     7261 tatagtatat tccctttggc tttattattt ttaatttaat acctaacata gtaataaagc
     7321 ttattattct ttctttcaat cgtaacattc cttgtccccc ttaaattttc acctaaaatc
     7381 caggcaatat atttggattc attattgctc catattctct atcattaaaa tttgaaatac
     7441 caagtattat atttcctatc ttttgctcat ctacttctat atagtcagct tttaagccta
     7501 ttattatgct tgtcttatta cttccagcat gtttataagg tattattcta actctacctg
     7561 aagttttatc atctaaactt cctataattt cttgtgcttt cttcatatct atatcttcat
     7621 aattaatatt taatagtttt tctggaataa atccttctaa cacactagac ttaactatta
     7681 ttacatcact ttgacttatt gggtctttta acaaattacc actatctact aaagctttac
     7741 agcaaaaact tttttctagt aagtttatat ttatagtctt tgtaagctcc ttaatgtact
     7801 taagcatttt caaatcttta taaatgtatt ttaataattc acaactcaca taagtacatg
     7861 caattaagaa agatattttt aagtgactta ttcctgtaaa ataaattata aaataagtgc
     7921 ttccagaaat aaaaatgttt actagataaa aaactaacgc tacatgaata taatcttttt
     7981 tgctcttact tctaaatgaa attaaagtta ttatgctcat gattattatt ttaaatggta
     8041 cagtaaatag tatattcatt gatggataca aataagcaac agaataggca cctcctaaac
     8101 tagcccccaa taacttattt tttttaggat tatatctttt agtaagaata gaagtacaac
     8161 ttataataat ataatttatt actaggtttt ctataacata gtactcaata tacaccatgt
     8221 atcccccctt aaaaccttta aacatattat aagatagtat gtacaaaatt ttatgtcact
     8281 tttgatggtc gaatccaaaa caaattctat ttaaaaagaa caatgatata tgtcttatac
     8341 tttatgtcgt caaaagatat ttttttaatg ccaaacaaat agactttttt gtagaatcca
     8401 atatatctag gtagaagtta ttcgctctac ttttggtata tttacaaaaa aagactacat
     8461 aatttaataa tatatcaagt aaatctttta cttatataaa atcaaattat atagtctgta
     8521 aattcaaata taaattattg tgctagtaat ccatttgcat attttgcaga ttcatttagt
     8581 gcttgttctg gagttatgtc tccagctatc atcatttgaa gttgtttcca taaatattca
     8641 tatatctcta ctccatgagg tccaactgtc aatggtctat acatatgttt atctcttgtc
     8701 accatttctt caaatcttgg gtctccttgg tatttttcac ctttagcaac tggagctcta
     8761 ggtatttctt tgtggaaagc ttccattgat tctttgctta gcatatactc aagaagttta
     8821 aatccaagtt ctttattttt acatgctgac attaatgtta aatcatctac tgaagcaaaa
     8881 cttccaactg tcttatcttc taatgatgtt acatatcccc agtttaaatc cgggaatgat
     8941 ttatcaaaag tacttgtagc agcagaggat agcattatag taaatgcagc ttttcctggt
     9001 ccaaatacac tttcaatcat ttcattacta ttttttgata aagcatcttc tggaatatac
     9061 tttttcatat catttataaa gtttgcagct ttcaagcctt ctgcattatt aaatttaact
     9121 gattttaaat catcattata tatatctcca ccagcttgcc ataaatacgg ataccagttc
     9181 cagtttaagc ttccaaatac tttagaaccc caaccttgtg caagacccca ctggtcgatt
     9241 tttccatctc catcagtatc ttttgttgct ttttcacaga ttcttttaaa atcatcccat
     9301 gtttttggag gtttttctcc taatttttct agtatatctt tattgtagta caatacgcct
     9361 ggattagcag cttctatagg taaaccataa ataccaccca tcatttttgc atcatctaag
     9421 tatagataat tatctgaagt tcctgatttt tctaaataag gtgttaaatc ttctaccgca
     9481 cccatttcta taaattgagg aaacatctct gcatacatgt atcctatatc tggaccttca
     9541 cctgcactta ttgctgttgc atatttttct gcgtagttat cccatggtac tatctgaaat
     9601 tcaatttcac agttattttc tttttcaaat ttttcaaata tcggcttcca tgcatctgca
     9661 tcatggtctg ttaatggtgc agaccataca actaattttt ctttaccctc tttgccttta
     9721 gaatcttctt tttttgaaca tcctgcaaga attgatactg atagaatccc taccataact
     9781 aaagaaagaa ccttttttag tttaatcatg cttaatcccc tttcagactt taatttgtct
     9841 ttttcctcaa atgtttacat ttaattcaga tttaatatta acaaataaga ttactcttgt
     9901 ctaatcataa aaaactatac taactcctat aactatattt tttttctata cctattagat
     9961 ttaattcata ctaaaaactt actttttaaa ttttatcaat atttaaaaat atttttaagt
    10021 ctagccacaa actaactatc taaattgaac aaaataaaaa gaagtctatt gacttcttta
    10081 taagcttaca tattatcttc ttcttcttag gaatgtaggt atctccatgc tctcatcatc
    10141 atcttcttca attatacttg cctttttagg aggctctatt ttttcctcag caattacttc
    10201 ttcttcaatt tcttttacag cttgttttac tgttgtattt aatgaagaac gtatgcttga
    10261 tttaggttta gtatctaaat ctaaggcacc attttgtaat ccttcaaatc ctgtcgcgat
    10321 tactgtaatc attatttcat cacctaaatc ttcttttata gaagctccaa atataacatt
    10381 tgcttctggg tcacaggatt ccataactaa agttgatgcc tcattaactt caaataatcc
    10441 taagttagga ccacctgtaa cattaagaag tactccttta gctccttgta ttgatgtttc
    10501 tagaagtgga gattgaatag cttgtcttgc agcatcaatt gctctagttt caccactagc
    10561 actacctata cccatatgag caagtccctt gtctttcatt atagtagtaa catctgcaaa
    10621 gtccaagttt attaaccctt caactgctat aaggtctgaa attgattgta taccttgttt
    10681 taatacatca tctgcaactg caaaagcatc taacattgat gtattttttt gtactatctg
    10741 taaaagtcta tcatttggta tcgttataag agtatctact tttgatttaa gttctgctat
    10801 tccaccttca gcgttcttca ttcttatctt accttcaaat gcaaaaggtt tggtaactac
    10861 acctactgta agtatcccca tttcttttgc caagcctgca accactggag cagctcctgt
    10921 accagtacca ccgcccattc cggctgttac aaagaccata tcagctcctt gaagtaactt
    10981 tactatttca tctttactct cctcagcagc tcttttccct acttctggat ttgctcctgc
    11041 tccaagtcct ctagttaatt tttctcctat ttgaacttta tattcagctt ttgatgtata
    11101 taaagcttgt ttatctgtat ttactgatat aaattcaacg ccttttagtt gagcttctac
    11161 cattctattg acagcattgt ttccaccgcc acctacacct attactttaa tttgtgcaca
    11221 ttcttctaat tctacgtcaa agtttagcat cataaacccc cttctctcat gattcaaagt
    11281 tatcgtatgt attaattata tcattttctt cttttatttt gttaaccttt aagtaacata
    11341 ttccacaaaa acgtaaattt ccctttttaa atttcttact tatattgtca aatattactc
    11401 tttaagtata aaataattta cgtatatatt aaatgctctt taatttttac gttttttgtt
    11461 cataatatta ctaataatat atcgtctaat tatagataag ttattaaaca atctcatacc
    11521 aaaaactaat acagcaacat agtaaagaga aactcctaac ttttctccta tataagtcaa
    11581 taatatagcc aatatagcat tacctatgaa accagtaata aaaattacat tatcatagtt
    11641 atcttcaaaa ttggatttta tcgctccaaa aatagaatcc attattgcaa gaatagctac
    11701 tgacatatat atggaataat ccatagaata tgtaaatgga atatagtaac ctactactac
    11761 accaaatgct aatcctccta aaacccataa cataaatctt cacctcttta ttttactttg
    11821 aaacacaact acattacaat cctttttatt aatttttttt actttttctt tatttttttg
    11881 tatttttcgt tttattttgc aaataaattc actttatttc cattattttc ctgaaccttt
    11941 atattaattc catatatatc cttaagagat ttagcatatg attctggaga gtttatagct
    12001 gcactcaata aatctatatc tcctatagct tttattataa atggttgtcc atatgtagta
    12061 tcatttactg taatagtagc tcctgaacat tttatcttac ttgttgctag tactctttcc
    12121 ccatttatag aaattgctct tgctccagcc ttttttaaat catttaaaac tctcaaaata
    12181 tctatatcat gtataatcaa atcgtttgga ttctgtccgt cttttaactc tctctcacta
    12241 tccctcatag taatagttat gcctggccct gtaacagttg aataaccact taattcttta
    12301 acttcttcaa gctctttttg cataagtgtt tggatagatt catttccatt cttttttgct
    12361 tctttatatt cattcaaagt ttttttgttc tttttaagag tttcttttaa attttttatt
    12421 tctttttttg tattttctac ctcactttca atactttcag tttgacctaa tgttatgtat
    12481 actctatttg ggtctagtga ttttatataa gttgatatga aaacgcctaa tatgaatgct
    12541 cctaatataa ctattttctt ttgcatacta ttatttcccc tctgctttgt ttatatcact
    12601 atgaccattt atttctatat tgtcttcttt ggtcactttt actttggcat tataatatgt
    12661 ttctatttcc cagacaatac catactttat attaagtgcc gaagccaatg tatcagaatc
    12721 accaattgct tttattataa ttggttcttt tatgacagtg tcattcatat aaagcttgtc
    12781 ttcttttaaa tacatatatg tattagaaac tattctctgg tcgtttatag atatggcact
    12841 tgcttgtgct gaatttaact tatttatcat tgacaataat aaatcataat tatatagtat
    12901 actatcattt ttatcagagt ttcctaagtc ttttgtagaa tttatctcaa tttttatgcc
    12961 tgttccttca acatctgtat atccagctaa tgtttcatat ttttctattt ctgacttaag
    13021 tatgtcttgt tctccttcat tgtttttgcc tctataagtc tctatatctt tcttcacact
    13081 attaacttct gattgtagat tctctttttc tttttttaaa gatttaagtt cttctgtaag
    13141 ttgctctcct cttttaaaag ttgtcatccc tctattttct atattaataa ttttaagttg
    13201 taaactaaca agaacaccca atacaatact tcctatgatt atacttttat ttataaatct
    13261 ttttttcaat atatcacttc cttacaattg tctatccttc tggactatac actgccaact
    13321 ttccatagtt aagatttata gtccctccat tagtattttt attttgcagg tcaatcagaa
    13381 ttttacttac cctagatata ttataatcta agttatcatc attagaaagt aagattttta
    13441 tattacttct tgtaagcatt tctatattgc tttctttttt taagtttaca taatttattt
    13501 ttctataaat tccttcttct tttaaataat ttaaagtttt aaatacattc tccttagttt
    13561 tataattttt aaattttata gatttattat catcaatgct ataatctaca tctactatca
    13621 agtctttttt actttcatta gaacctctta gttcttccaa taaatttcct tttttatcta
    13681 tataacaata attatcttca tcttttaata ctgcaaatat ttctttttct tttaaactta
    13741 taattatctt attgggaagt tttcttttta tttccacatt ttctatatat ggatttttta
    13801 taagtttatt tttcatatct ttaaaattgt atgcaaaaat attttcattt agatttacat
    13861 ttagttcttt cattatattt gatttggtca ccctcttatt tccaattaca tctattttct
    13921 ttacatcaaa taaatcactt tttacaatta tacatataca aaaagataac ataagtataa
    13981 aaactaaaac tatcatgaca ttattcgtat ttaactttct tctttttttc aaatataatc
    14041 aacccctttg gttaataaat acacctttag agcttgtttt tcctacaaaa aaattataat
    14101 atttaaagtc aaatttttgc attttattac ataacatcca aataaggtat taaattttat
    14161 aaataaatca atatttcatc aaaataaaca tagcatacta catactattt atatatttaa
    14221 atctgtatac atacaaataa acccctaatt tataataatt tagaaaatct aatatattat
    14281 aaattagggg taaatattta cttatctttt ctttattcct attaccttag cttgaccttc
    14341 tatacttgga gtcgtttctt tttttatttc ttttacttcc ttaataactt tttctttttt
    14401 tgttttttta gaagttgact tttgtgttga attgtatact ttcattatct catcatatat
    14461 aaggtctatg gcttcaggct tacctatagt tttacttgca tttgccatat caaccaaaag
    14521 ttctctatct cctaaaagtt taaatactgc tgtatttagg ctctctggag ttagattttt
    14581 ttctaaaata gcaattcctg ccccttgttt ttctatactc ttggcattat actcttgatg
    14641 attttcagca gtataagctt taggtattat tatagatggc tttccaagag ccgttatctc
    14701 agctaatgaa attgctcctg cgctacctat aaccaaatca cttgctgcaa gtgcatttgc
    14761 catatcttct aagtaaggca caactttttg gtatggttta agatttatat cgctaatact
    14821 tcccataaat tcatcataat agcttttgcc tgtagcaaat ataaaagcta tatcttcatt
    14881 taccatattc tttattacaa gtctcattgc atcatttatt tttcttgaac ctccactacc
    14941 accatagcaa agtaccattc ttttttcgtc acttattgat agattctttc ttgcaatatt
    15001 ttttcttgat aataaaattt cttttctaac tggatttcct gtaaaaacta atttatcttc
    15061 tgctgcctct ggaaatcttt tatgcgaatc ttcaaaactt gtaagtacct ttgtaactgt
    15121 tttagataaa atcttattag ttacaccagg aaaagaattt tgctcatgta ttatggctgg
    15181 tattttcccc atagatgcat taaataaaac aggtccacta acatatccac cagttcctat
    15241 aactatatct ggtttaaatt tcttaacaat ctttcttgat tgctccaaac ctttaaaaag
    15301 cttaaaaact ctttttacat tatcgaaatc tatttttcgc ttaaaacctt gaactgttac
    15361 agtctttaat tcaaatccat acttaggaac tatttctgac tctattccct tttccgtccc
    15421 aacaaaaatt atctcagcgt ctggatgctc atctcttatt ttgtttgcaa tagctatagc
    15481 tggatacacg tgccctccag ttccaccgcc tgataataaa actttcatca aatcatcatc
    15541 tcctgttaat taattttgac atgctttgag atgtttaaca ctattcctac agctcccata
    15601 aagatggtga gtgaagttcc tccataactt ataaatggta gtgctacacc tgtagctggc
    15661 attgatgatg tcgcaactgc tatatttaaa gctgcttgta ttccaatttg tgcacctata
    15721 cctataacaa ccatacaagc aaatacatta gaagtcttca aagctattct tacacatctg
    15781 tagactaaaa ctacaaatag cataataact attatacaac caatcaatcc taactcttct
    15841 cctattatag caaatataaa gtcattttgt ggctctggta tataaaaata cttttgttta
    15901 ctctttccta agcctagacc aaacaatcca cctgaaccta gtgcatatag accttgtatt
    15961 acctgatacc ccttacccag agggtcttga aatgggtcta aaaatgatgt aacacgacta
    16021 agtctatagg gttcagcaat tactaatgca gcaaataaag cagccccact ccctatcata
    16081 gcaaatacaa ttttcatatc cattccagca acaaatatca ttacaaaagt aactagaatt
    16141 acagtacctg ctgttgacaa atttggctga agtattatca acgcaaaaaa tatccctgga
    16201 actacgagaa gtggtacaac accttttgtc aaagatttta ttttatcata atttttttca
    16261 attaattttg cagttaatat tattgtagca aactttgcga tttctgcagg ttgaaatgtt
    16321 aaagctccta ttcccaacca tctttttgct ccgtttgctt ctatccctaa aggggtcaaa
    16381 actaacaata ataatactac tgctatagct ccaattgctg tagcattttt cttccaaaaa
    16441 ctataatcta ttcttgatgt aataatcata actataaatc caagtacagc atatattaca
    16501 ttctttttta gaaaatagta cgcatcattg tgtttaaatg aagattgtat aaaacttgca
    16561 ctaaacacca taactattcc tacaaatact aaaagcatag tagtgtaaaa cactactcca
    16621 tcaaattcag tcttcatcct tatgtcaatt tgttttttca aattttcttt aggcatccaa
    16681 ctaatctacc tccattcttc catttaagta agtttagagg taagagatta ttttaaattg
    16741 tttacattat ctttgaagtc ttttcctctt acttcaaaac tttcatacat atcccaactt
    16801 gcacaagctg gtgaaagaag cactatatct ccactttttg aaatttcata agaagtctta
    16861 actgcttctt ccatatcttt tactatatgt atgtcattaa atcctttatt tttagcacaa
    16921 ttttctatat ttgatgcagt ttctcctaat aagaccaatg atttaacata tgactttgca
    16981 gtttcaagaa gttcatcaaa attacttccc ttatccatcc cccctgctat aagtattata
    17041 ggtctatcat aagactgtat agcttttata gtggaatctg gattagttcc ctttgaatca
    17101 tttacatata ttacattgtc taaatttctt acaaactctt gtctgtgttc cactccttta
    17161 aatgtcataa gtacatcctt tataacttct aaatcaattt tgcagacata tgctattgct
    17221 atagctgcca tacaattttc taagttatga cctccaggta aacttacatc atctttattt
    17281 agaaatttga ttttatcatc tatatctatt attatgtcat tattttctaa atatactcct
    17341 ccatttactt ttttagttct tgaaaagtaa attactttgg cattgcattt atcagctaaa
    17401 gattttacta tctcatcatc atagtttaat acacagaaat cctcaacagt ttggttcata
    17461 aatatatttg cctttgcttc tatgtatttt tccatagtat ggtgtctatt taagtggtcc
    17521 tctgtaatat ttaatattgc gcttacttta ggtctaaatt catctatact ctcaagctga
    17581 aaactgctta gttcagttac taacacagac tcttcacttg aagtttctat agtatctatt
    17641 acaggatttc ctatattacc aactacatat gtatctcgtt ttgctcttga aaaaatttct
    17701 cctaccaaac tagttgtagt tgtctttcca tttgtacctg taatccctat aaaaattgga
    17761 ttattcgata gtttaaaagc taattctacc tcaccaatta tatacttgcc tgaatttttt
    17821 aatttcaata tgaatggtag gtctagtggt acacctggag acactacaac catatctata
    17881 tcatctacat cttctgggtg atgtcctaat atgtatttta tgtcatttat agatttcaat
    17941 tcatcaagta tatttctcaa tttattttca tcttttatat catttacgat tatacttgca
    18001 cccaatttat ctaaatgttt aattgtagat ataccagttt ttgcaagtcc tactaataaa
    18061 actttttttc cttttaagtc catttttcat tccccctagc cctaaaatac tgctattata
    18121 cttatccaag ctaaaacaac tgttataatc caaaaaataa atacaactct tgtttctggc
    18181 caaccacact gttcaaagtg gtggtgtatt ggagccatct taaagaatct ctttcttgtt
    18241 gccttaaaat atccaacttg aatcaaaact gatagagcct ccgcaaaata aattcctcca
    18301 attataggta ttataagcac tgagttagtt agaactgaaa atgctacaac tgcacctcct
    18361 aatgccattg aacctgtgtc gcccataaac actctagccg gatatgaatt aaatcctaag
    18421 aaacctaaac atgctccaac agttgctgct gctaaaactg cgacttctgt atttccagca
    18481 atagaacttg caaatagcat aaagaataca gaaactatta atgttactcc tgatgctaat
    18541 ccatctaatc catcagtcaa gtttacagca tttactattg ctacgattat aaacatcatt
    18601 attggtatat ataatattcc aacatttatc acaaaatctg taaatggtat cataagttgt
    18661 gaagcacttg atgatgatgt atactgataa aaagcaacat aaaaagataa tgcaacttga
    18721 aggattattt tttgatatgc ttttagtcca agtgatcttt ttagctttat ttttataaaa
    18781 tcatctaaaa agccaataaa accaaaacct gcaatacata taagacctac tgccatatca
    18841 tgactaactt ttactcttgt aagtccagtt attagaattg caacaatcat tataattcca
    18901 cccatagtag gtgttccgtt tttagctaga tgtgtttggg gtccatcatc tctaactgtc
    18961 tgaccaaatt taaattttct aagcattggt ataaatattg gtcctattat aattactatt
    19021 aaaaacgcaa tcaatgcggt gtacgtaagc tctgttattc ctaacatcat aaaactcctc
    19081 tcccttgtca attaactcat tagtttaagc ttatttattt agatattcga ctattttttc
    19141 taatctcatg cctcttgagc cttttactaa aatagtgtct cctgttttta ctaattcatt
    19201 taacttgtta aacacatctt ctctattctc aaagtgatat atattggctg aattgaatcc
    19261 taattgtttt gcttcttctc caataaacac tgaattttca cctatagtta ttataatatc
    19321 agtattattc atagatgatt ttccaactaa tctatgacca tactctgaaa tttcacccat
    19381 ttctagtata tctccaagta tcgctaccct tctaccttta tatcttccaa gtatctttaa
    19441 agcagcatcc atagaatctg gactagcatt atatgaatca ttaattattg ttaatttttc
    19501 attttttatt atgtctagtc tcatttttgt ccctttaaaa ttttttaaac ctttttttat
    19561 ataatctaaa gaaatattta gacacaaacc aactaatata gcagacattg cattatatat
    19621 attatgttct cctattgttg gtataaatat ttcctctttt ttcccattta ttacacatat
    19681 aaatgttaaa ctatcctctt ccatagtata actttcacaa taaacatcat tgtttttatt
    19741 aaagccaaat gtttttaact tatatacatg ttctttttct tttaatgtag ataaaaactt
    19801 atcatctcca tttacaatta aagtatttgt ttcatcaaag tttgaagtaa tctccatctt
    19861 tgctttcaaa ataccctctt gtgaacctag tttttctaca tgtgataatc ctatattcga
    19921 tatgactgca attttaggat ttactatatc cactaaatat tttatttcat tgaatccaga
    19981 catacccatc tctattacag cacactcatg ttctttattt agattaaata aagtcaatgg
    20041 aactccaaaa tgattattta agtttttttc attttttagt atatttaact ttgcagatat
    20101 ggcagagtaa atcatatctc tagtagtagt ttttcccaca ctacctgtaa cacctataaa
    20161 tggaatatca aatttttctt tatagtattt tgaaatatct cctagagcca aagatgtatc
    20221 ttttacctct attaaattta tatccgaact ttctaatttt attccattac tttcattttt
    20281 tataaaagtt ttacatccac tatcatatgc agacttcata aatgtatgcc catctaaatt
    20341 ttctccaact atggctacaa atgcattttg ggcatttgcc tccctgctat ctataactat
    20401 atcacttaca cagtcattat aatctccata tattaacttc ccattagttg caagtactaa
    20461 ctcttttatt gttaagcact ccattattaa actcctcctt taaaacctta gctatattct
    20521 cttagcaaca aaaggcaatg tataccgtta tatatacatt gcctacgata actcttaact
    20581 ttataattat atactatatt tctgtttttt tgaagatgtt tatgggtata tgttaattat
    20641 ctgttacttt ttagttttca aaatataggg ttatacttga gtcttcatta acttttactc
    20701 caggttttgg gaatatatcc tttacctttg tatcttctgg aagttctata tctgcatcta
    20761 agtctggttt taacttcgca tcctccaatg cctttacagc atctcctatt tttagatttc
    20821 ttacatctgg cactttaact tgcttctttt catactcagc tttttcttct tctttatata
    20881 ctggtttaac tcccaaatat tttaaagagt cattcattat ttctttcact attggtcctg
    20941 ctgtagtact accaaatgca cttactcctg ttggttcgtc aacaatagca agaactacta
    21001 tttgcgggtc atcacaaggt gctattccaa caaatgaaca tatatacttt cctggagcat
    21061 acttaccatc tataactttt tgagctgtac ctgttttacc accaagtcta taacctggta
    21121 tatatgctat ctttcctcca ccttcagtta caactgattc tgcaatttct aacatttttt
    21181 tagatgtttc tttagatata acacttctta ctttttttgg ttttactgtt tcagttatat
    21241 ttcctttatt gtcagtataa gattttacca ctcttggttg cattaaatct ccaccattag
    21301 ctattgatga tatagctgtt ataagttgta ttggagttac tgatatagat tgaccaaatg
    21361 atattgtagc caattcaact ggtcctacat ttttttcgtt atacagtatt cccttagctt
    21421 ctcctggtaa atctatacct gtcttatcca taagaccaaa tgattctata taatcataca
    21481 tttttccaac acctaatctg cttcctaact caacaaatac tgggttgcaa gagttttgta
    21541 ctgcttgttt aaattcttgt gttccatgag gtctgtaatg tctccaacat ttaatctttc
    21601 ttcctccaac agttacacta cctgtacatg tgaatttttc tccatcttta attactcctt
    21661 cttcaagagc actggaagat gtaataagct taaatgtaga acctggttca tatgtatcac
    21721 ttactgctgg atttctccac atttgataat agccttttat tttatcttta tcattatatt
    21781 tttcaagttc ttcttgataa tatggatata ttggcgttct tgagtcatta gggtcataat
    21841 ctggttttga tgctaaagca agtatatcac ctgttttagg gttcatagct attgcagtaa
    21901 ccttttttgc attatttaac tcataagctt tctgtactgc tttttcagta tagtgttgaa
    21961 ttacttcatc tatagacaac acaagaccat taccttgtac aggctcataa tatttttcca
    22021 ttccttgtgg tatttctctt cctgaagcat cagtacttac tataagtttt ccaggctttc
    22081 cttttaactt tttatcatat tgcatttcta caccagatat acctgttgca tctgaagaag
    22141 tatgtcctaa cacatatggt gcaaaattcc catatgggta atatctttga ttatcttctg
    22201 ccacccatat tccacttaat ttagcatctc ttatttgact tgccttatca tcatctatcc
    22261 atctttttac ttttacaaga gccatatttt ttttgcttat taaagcgtaa atatctttat
    22321 aatcttcacc caaaatctca gctacctttt cggctgcttc ttttttatct tctacttcca
    22381 caggtttaca ccaaacagta tacttagtta cacttactgc taactctttc atatttctat
    22441 catatatagt tcctcttttg ggttctattg gtatatctct tgtttgttgt tctagtgctt
    22501 ttgtactcaa ccaatttccc ttcataagtt gtagataccc agtcctaata acaaggcaaa
    22561 aaaacaatgc acatgctaga ataagaacga gtaccagcct tttcttactt atcctcttta
    22621 cttttctcaa atctattccc cctagttatc cacatctata tactttattt gtccatcttt
    22681 tggatagtcc atattgagca attccttagc ctttagctca atttctttta ttgatgtttt
    22741 ttcagtttct tttgccttta gttcctctaa tactatctct ttttttctta aatctttttg
    22801 taaataatag atatcgtact tcatttccga gatttttgaa taaccacata gattcaatat
    22861 tattactgaa aaagttgcaa tcagtaaaat gcatttagct cctatttttt tcttatttct
    22921 ctttttataa ttattctttt ttttattttt atattcattt agattatgta ttttataatt
    22981 ttttttcaaa ttctcacccc gatagtatat agtttaaatt ttcctctata ctatatttgt
    23041 tattaatcat atttcttcct ctaaaatttc atcgctaaat taatacacta ttacacaatc
    23101 taattgtatt tttttctata cacaacaaaa acctataaat tcacttatac ttttatatga
    23161 atctataggt tagatattaa tttctattta tttttatcac ttctagtatt tttagataat
    23221 ttcttccata aatataatat gtaactaact atctactctc tgattcctaa gttctccatt
    23281 atattactta ataccatcta tatctatatt tttatatcta aattttcatt atatatctca
    23341 ataacaaaga acatttctta tagtgttaat tgttttttaa ttgctttacc agtgcaacaa
    23401 aatatataat actttttaat taattataat aatcttatat ataagattat tctttagcct
    23461 tataactact tatatttttt ctgctactct tagcttagca cttcttgaac gtggatttac
    23521 ttctatttct tcctcactag gtaatatagg ttttctagtt attattttca cctcagattc
    23581 tttatcacat tggcatatag gtaaatgctg tggacatata caatctgatg ctaaatgctt
    23641 aaatgcattt ttcactattc tatcttctaa tgaatggaat gttattatac atattcttcc
    23701 accttcattc attatagaag aagcatcatt tatcatcttt gtaattactc caagttcatt
    23761 atttacttct attcgtattg cctgaaaagt tcttttagct gggtgaggtc catctattct
    23821 agctttctta gggatagctt ttttaattac atctactaat tctcctgtag tttctattgg
    23881 tttgtttgct ctttcttcaa ctataaattt agctatacgc ttagcccagt tatcttctcc
    23941 ataatctttt attatcttag ttaattcatc ttctgtataa gtatttacaa catcatatgc
    24001 agaaaactca cacctaacat ccattctcat atcaagtggt gcatcttgca tataagaaaa
    24061 tcctctatca gcttcatcaa gttgatgaga tgaaactcct aaatcagcta atacaccatc
    24121 aattttgtaa actcctattt tttctaattc ctcttttata ttttcaaaat tgctgtgaac
    24181 aaacttcact ctactttcat attcactcaa tctctctttt gctgtgctta tagcattttt
    24241 atcttggtca aaccctataa ataatccctt atctgacagt ttttttacta tttctttaga
    24301 atgtcctgct cctcccatag tacagtcaac atacacgcca tctggcttta tattcaaatt
    24361 ctctatacac tcatttaaaa gaactgatac atgatgaaat tccatagtta cctcccttaa
    24421 attcactttt taaactattc ttcactaata ttattcttat ttttctttga taaataaaca
    24481 tgtgcaatta tgccaccaat aactccaatt agacttataa cttgtgccat tctaagtggt
    24541 cctaacatta aactatctgt tcttaatccc tctataaaaa atcttcctat tgaatataaa
    24601 gttatataag taactataac ttgaccttca tattttttat tttttctaaa tagtaataaa
    24661 actataaata ttccaaaatc ccatatggac tcatataaaa aagttggatg tacctttact
    24721 ccatcaacca ttattcccca aggtaaattg gtagcacctc catgtgcttc tccattaata
    24781 aaatttcccc atcttccaat agcttgtgct aaaggcattc caagtataac tgtatcagcc
    24841 atttttaaga agttaatatt tttaatctta gtgtatatat aaccagctaa tattccacca
    24901 attaaccctc catgtatagc aagtccacca cctctaaaat taaaaatttg agacatattt
    24961 tgagcatagt aactccaatt aaatacaaca taatagattc ttgcacagat tagtccaact
    25021 ggaattgcta ttatagctat atttaataca tcatcttctt taattcccac tctttttgct
    25081 tcttttattg ctattaaagt tcctaaaatc attccacatg ccataagtat tccataccac
    25141 atgatgtcta tgccaaacag tgtaaatgct actctatcca taacgtcacc tctttgtatt
    25201 aattgaaaat atttccaaag caatatgcta taattacaat taattgtaat tatactaaat
    25261 tatgataaca cattaatgtt aattctacaa aaaatattaa aatttattct acaactccct
    25321 acactatatt atatctaatt actataaaat tttttaaact gaatctctca tgactaaagt
    25381 tacaagtgta catagatatt ttttaatcaa aaagattgat gcatatatac atcaacctct
    25441 tctatttctt atctataact cttaaatttt tacctcaatt tatataaata tattttataa
    25501 atttgtttta ccaaaataaa catttaaaaa cataaactaa tacttaatta gttattctta
    25561 ttatgattaa gtttctattt aggctctaca atagaaataa cacaatactc ttctttaaaa
    25621 tgctctttta atctttcttc aacttcctca aaagtaatat ctttaataac atctaaataa
    25681 tccaatagat ttatatcctt aaaaacataa gatataaaac tattacctat aaagtttata
    25741 gaatcaaagc acttaataaa tgaccctatt tttttctttt tagttctctc aaactcttct
    25801 tttgataatc cttctttttt tgatttttct atataatcaa gaattatttc ttttactttt
    25861 ttaggctctt tagaatctcc agctattatg ctaaaagcat aatccacttg agacgaaaat
    25921 ccagctccaa aattttcatt tattaaacct tgcatataca aatcttcata aagcttactt
    25981 ccttttttaa ataacatccc tacaagtata tcagttacaa tctccttacg taaaagttct
    26041 ttccctttta aaccaacatt actatcttta aaacctatgt tgaacatagg catagatata
    26101 gggaattttt ctataacctc tttttctttt acaccttctg gttcttctgg ataaaatctt
    26161 tctatactct tagagagttt gtctactttg taattgtttg actttttagt tacatctata
    26221 actttttcta catctaagtc accaacaaca aataaagcca tatttcctgg attatagaaa
    26281 gtattataac atttatatag ctcttcctta gttatcttat atatactatc aacagtacct
    26341 gctatatcaa ttctagcagg ataatttaca tacattgcct tcaagcaatt aaagtataca
    26401 ttccaatcag ggtcatcatt gtacattttt atctcttgtg ctattatacc tttttccttt
    26461 tcaacatttt catctgtaaa ataaggtgtt tgaacatagt caattagatg ttctaaactc
    26521 tcataaaaat tttcagtagc agaaaataag taggctgtca ttgtaaaatt agtaaatgca
    26581 tttgcattta ctcctaattt tgaaaattta tcaaatgcat ctcctccatc aggttgctca
    26641 aacatcttat gctctagaaa atgtgcaata ccttcattca ctctaatttt cttatcttct
    26701 ccaattggta caaattctaa gtcatttgaa ccataattag tagccaaaat agcatacttt
    26761 ttcataaaac cccttttagg cataaaatat acatctaatc cattttgcaa tttctcataa
    26821 taaacttctt ctttcaatat atcattaact attttttcca tagacatctc ctccattaat
    26881 ttctcaagaa gtatacagta tcaagctcta tattttttat agcttcaact atatcttttt
    26941 ttgtaacctt ttctatacta gatattatat cttgtacttc agaatttgtc tttgccatag
    27001 attgagagaa aacaaaatca gacataccac ctatattatc agttatagat ttcatagaat
    27061 taactaaagc aagtttactg ttttctaatt cttcatcaga tatatcacct gcttttacac
    27121 tttctacttg tttttttatc aattctacag ctttatcata atctttagtt tctataccag
    27181 atgatataaa catgatactt ttatacttct ctattgaaga gaatatatag taacataaac
    27241 tttctttttc tcttacatta acaaataatt tagagtgtgg accaccacct aagacattgc
    27301 tcccaactac taaagcataa tatttatcta tgtctgcata atccacattt gctctataac
    27361 ccataactaa cttaccttga gttatttcca tttgttcatc tataactttt acctcatcca
    27421 cattttttat aaaatctgct cttggtatat ttataatttc ttctctttca aattttagat
    27481 ttttacttat tatatctaca actttatctt catcaaaatt accttctaca actatatcta
    27541 taggtgatgt tttaagtatg tttttataat gttcatataa ctctcttgaa gttatactat
    27601 ctatttcatc ttcataacca aattcactta cactatatct ctctccttta cacatagcct
    27661 caatacattt atcttgcgca tattcctttt tatcatttat tattgattgt atcttttctc
    27721 ttaaattctc tttttcaata tttaaatatt cttctttaaa cccaccatct acaacaagtg
    27781 tattatttat tacttcatta aaaaattcta ctacttcttc aaatatactc tcatctaaat
    27841 acttttcact tatgttaatt actttaaaac tcaatacttg acgttctcca cgttttacag
    27901 catcagctcc catagatgaa ccatataaat catctaattt attggatatt gctctagcac
    27961 taggatattt agcgcttcca cttgctatta ttgatggtat tagagcattt tttgtagttt
    28021 ctttttcatc caatagtctc tgaacatata aacttataag attcgattta aacttttctg
    28081 tttttatcaa agtaagatta atattattac ctaaatttat tattttttta gtattaacca
    28141 tatgtacctc ctatttttat ttaaacatca attttaaaat tgaaatttat ttatttttta
    28201 attacatata ctcacatata aaaaccttct atttaattat atccttttta ttattttcta
    28261 aaagcaatat tttt
//
LOCUS       DAEMHW010000035        28083 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEMHW010000035 DAEMHW010000000
VERSION     DAEMHW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.035"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(79..1158)
                     /locus_tag="KPK67_001298"
     CDS             complement(79..1158)
                     /locus_tag="KPK67_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2458291.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      1446..1561
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1736..3106
                     /locus_tag="KPK67_001299"
     CDS             1736..3106
                     /locus_tag="KPK67_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2458292.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(3371..3982)
                     /locus_tag="KPK67_001300"
     CDS             complement(3371..3982)
                     /locus_tag="KPK67_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBG2458293.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(3972..6335)
                     /gene="lon"
                     /locus_tag="KPK67_001301"
     CDS             complement(3972..6335)
                     /gene="lon"
                     /locus_tag="KPK67_001301"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBG2458294.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKESFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            6515..7027
                     /locus_tag="KPK67_001302"
     CDS             6515..7027
                     /locus_tag="KPK67_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG2458295.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            7015..7542
                     /locus_tag="KPK67_001303"
     CDS             7015..7542
                     /locus_tag="KPK67_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG2458296.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(7613..8863)
                     /gene="clpX"
                     /locus_tag="KPK67_001304"
     CDS             complement(7613..8863)
                     /gene="clpX"
                     /locus_tag="KPK67_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBG2458297.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(8882..9466)
                     /gene="clpP"
                     /locus_tag="KPK67_001305"
     CDS             complement(8882..9466)
                     /gene="clpP"
                     /locus_tag="KPK67_001305"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG2458298.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(9616..10902)
                     /locus_tag="KPK67_001306"
     CDS             complement(9616..10902)
                     /locus_tag="KPK67_001306"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBG2458299.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(11096..11566)
                     /locus_tag="KPK67_001307"
     CDS             complement(11096..11566)
                     /locus_tag="KPK67_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2458300.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTHMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(11566..12915)
                     /gene="rph"
                     /locus_tag="KPK67_001308"
     CDS             complement(11566..12915)
                     /gene="rph"
                     /locus_tag="KPK67_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBG2458301.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNIPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(13075..15099)
                     /gene="ligA"
                     /locus_tag="KPK67_001309"
     CDS             complement(13075..15099)
                     /gene="ligA"
                     /locus_tag="KPK67_001309"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBG2458302.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGEE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(15285..16223)
                     /locus_tag="KPK67_001310"
     CDS             complement(15285..16223)
                     /locus_tag="KPK67_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBG2458303.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(16325..16747)
                     /locus_tag="KPK67_001311"
     CDS             complement(16325..16747)
                     /locus_tag="KPK67_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBG2458304.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            16935..18218
                     /locus_tag="KPK67_001312"
     CDS             16935..18218
                     /locus_tag="KPK67_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBG2458305.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(18296..18811)
                     /locus_tag="KPK67_001313"
     CDS             complement(18296..18811)
                     /locus_tag="KPK67_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2458306.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINQENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(18811..20187)
                     /locus_tag="KPK67_001314"
     CDS             complement(18811..20187)
                     /locus_tag="KPK67_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG2458307.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(20212..20763)
                     /locus_tag="KPK67_001315"
     CDS             complement(20212..20763)
                     /locus_tag="KPK67_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2458308.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(20821..20994)
                     /locus_tag="KPK67_001316"
     CDS             complement(20821..20994)
                     /locus_tag="KPK67_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458309.1"
                     /translation="MCTKKEEQKCSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            complement(21005..21838)
                     /gene="fdhD"
                     /locus_tag="KPK67_001317"
     CDS             complement(21005..21838)
                     /gene="fdhD"
                     /locus_tag="KPK67_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBG2458310.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYINTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(21887..24031)
                     /gene="fdhF"
                     /locus_tag="KPK67_001318"
     CDS             complement(21887..24031)
                     /gene="fdhF"
                     /locus_tag="KPK67_001318"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(23615..23617),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBG2458311.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDVSEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(24282..25340)
                     /locus_tag="KPK67_001319"
     CDS             complement(24282..25340)
                     /locus_tag="KPK67_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG2458312.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            complement(25551..26210)
                     /gene="cprR"
                     /locus_tag="KPK67_001320"
     CDS             complement(25551..26210)
                     /gene="cprR"
                     /locus_tag="KPK67_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBG2458313.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            26521..27150
                     /locus_tag="KPK67_001321"
     CDS             26521..27150
                     /locus_tag="KPK67_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBG2458314.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            27218..28051
                     /locus_tag="KPK67_001322"
     CDS             27218..28051
                     /locus_tag="KPK67_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBG2458315.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
BASE COUNT         8543 a         5070 c         3166 g        11304 t
ORIGIN      
        1 taaaatggca tagcctcaaa gctacaccaa tttactaaaa attatattat tattaacatt
       61 aattttaaac atatacgttt ataagttttc ctcatatttt aaaattcttt taattaactt
      121 atctcttaat ttccctactt catcaaatct catattagga ctatagtaac tttctataat
      181 atcatgttta gacttggctc tcttaagatt tagtactgcc caacaaatta aatcatctaa
      241 aactttttca tcaaatttaa tatcttcttc atatttaagt agtttttctc tatttaaaaa
      301 ctgatttaaa tcaattgtat cttcttcttt aaaagtcgta cttatagtta tgcctatatc
      361 taaatcagtt aatagtacag tttcaatttt ctctggaata agtggataat gataaacttc
      421 tacatttaaa cctttttcaa tagctttctt atatactttg tttaataatg tagttttccc
      481 agttcctatt tctccatcta aataatagac tttctttaca tcttttaaaa tgttttctgt
      541 ataatctaaa caccctacag gagttatagc tgaaccaaat aaatgctttt ctctttttag
      601 atttcctgtg cttttaactc catcaaataa cctttttata aattcttctg tgaccaaatt
      661 tacttttcca taatccatac aatcagaata tttttcttct atgtctctaa agattggttc
      721 tgctgatttt aaaaacttaa atgctctttt aaatgaatta ctaatatctt tatcataaac
      781 tgatatttct tgtttattct tttctaattc ttctgtattc cagtaatcac ctaaattaat
      841 tatctcatcc aaagctccag gtagctttgg gtccatagta tgtggggcag ttgcatccat
      901 aagtaccaca cctaatttct ttataactaa accatccaac gaacttgggt cagaaggaca
      961 atgatataaa tcaatatcat atcctctatg caaaaactct ttataaattt ttttcataag
     1021 tgaggatttt cctactccag gtccaccctt taaacaaaaa actcttttta cattttcagg
     1081 aattatatag tcaaaaaaag aatatgagcc ttgtgatgta tttccacccg ggaatgcctt
     1141 ctttaccttt ccttccatat tagcgcctcc tcataattta caatatctct taattaagat
     1201 attcatatta taagaatttt attacagctt tctgattttg ataaatttaa aacttaccat
     1261 ctaatactaa taacaggtta attttatcta attattatag attctcatac tgtgccttat
     1321 tctatctata aataaaattt aagtgtccat attgaaatat ttgtattgta atacagctgg
     1381 atattactta aatccaattg tttccattat aattttatgt taaaataata ttacaaaata
     1441 catctgtttt tcttcataaa cgggtgaaat tccctatcgg cggtaaaagc ccgcgagcct
     1501 tatggcataa tttggtcata ttccaaagcc gacagtaaaa tctggatggt agaagaaaat
     1561 agtatatgag tacctttatg taattttaca tgagtaatct atacaaatcc ttcaactacc
     1621 gtatttattc atgaaattag acacattcaa ggtacctaat atacaggtgc tttttttgtt
     1681 gtttatttta caattatatc gtacttataa aatctattaa gattggagtg ttatcatgaa
     1741 acaaaaatgg atagtattga ttatcatctg tattggtgta tttatgtcta ctcttgatgg
     1801 aagtatacta aatatcgcaa atcctacaat tgctgctgac tttaaaatta acatgtcaca
     1861 aattcagtgg gttgtaacag cttatatgct tgttgtaaca gctactatgt tatttttcgg
     1921 aaaacttggc gataaagtag gttcaaatcg tctatacaca ttaggatttt ttatatttac
     1981 aataggttct tttctgtgta gtatgtcaaa caatttatca acattaattt cttcaagaat
     2041 ctttcaggca gttggagcaa gtattttaat ggctactggg cttggtatag tttccaatgc
     2101 atttccagca aatgaaaaag gaaaagctat aggaatcact ggagctgtag ttggtatagg
     2161 aaatatgagt ggtcctgtaa ttggaggtat aattttagaa cattttggat ggccttctat
     2221 ttttattatc aatataccta ttggtattat agcagtcttt cttggcatta aatttctacc
     2281 taaacctgtt ttggatgaac aaaacaagag ctttgatatt ccaggtcttc tacttttcgc
     2341 atcttgcact acgcttatat tacttgcaat gaatgagaag ggtaatacaa gattgtattt
     2401 aggtataaca gcacttataa tcttcttatt gttggcatta agagaagtaa aatttgaaca
     2461 aagctttatt gacctacctc tatttaaaaa tagaaacttt actgtaggca atatcattgg
     2521 agtcgcttgt tattttcctc aaatggcagt atcttttcta cttccatttt atttagaaca
     2581 attaaaaaat ttatctccta tgatggctgg ttatgtaatg actgtacatc cacttattat
     2641 ggtacttata gcacctattg caggttcact ttctgataaa cacggtgcaa aaaacattct
     2701 aacagcatct ttttcattta tgaccatctc actagttgga atggcacttt taaaagcaga
     2761 ttctccattg tatcttttaa ttgtatgttt agttattttt ggacttggtc taggagcatt
     2821 tagttcacca aacaatagta gtattttagc agatgttcct cctcaaaaac aggggtacgg
     2881 aggcagtttt cttgcaacta ttcgtaattt atcatttgct cttggaacag cattttttag
     2941 tagttttttt gcacaaagtc ttacatacaa tcaaaagttt aaaagtcaca catcagccta
     3001 tgttatagct agcaatcagt cttattggat tgcagcatca gtatgtttta tagggcttat
     3061 actaactgtt ttctttatga gaaaaacaga taaatctatt tcttaaatgc tatttattgc
     3121 tgactacata agtcaataat tttttagttg aactaattta tactaatttt cccatacctt
     3181 actaaaattt attagtattt agcaaaatta ctgatattta ttgtttataa acaataagga
     3241 tgccaaaaaa agttcattaa aactatttta aggcatccta tattttgctt taatattaat
     3301 ataattaaaa ctaactatta ttacatttat tattcaaaca aatcttatgt caatatttaa
     3361 cattttaaat ttaatctact gttatttcat atccatgttg attgtattga tttacaatct
     3421 cttcccataa ttcttcaaca ccagtctttt ttagtgaaga aacaggaatt actttttcac
     3481 tactttccat ttgcaatttt tttcttatta tacttatgtg tttttgatat tgtcctctag
     3541 atattttatc tgccttagtt gcaataacta cacagttata tccaaagtgc tttatccatt
     3601 catacataag cttatcatct gctgtaggct catgtctaat atctacaagt aggaatatag
     3661 aacataactc ttgtctactt tctagatatc gttccattat acctccccac ttttcttttt
     3721 cagacttagc tatctttgca tatccatagc ctggaaggtc tacaaaataa aattcattat
     3781 ttattagata aaaatttata gttctagttt ttcctggtgt ttgactggtt ctagcaaagt
     3841 ttcttctgtt aagcaatgta tttattattg atgattttcc aacatttgac ctaccagcca
     3901 atgctatctc tgggattcct tctgctggat actgtgattt attcacagca ctcatagtta
     3961 tctctgaact tctaattttc attattttca ccactcttta ataaagcttg ttctaatact
     4021 tcatccatat gttctacaag cacaaactcc attttttctt ttacattttc tggtatttca
     4081 tctaaatcag cttcacattc tttaggaatt aatacttttg ttataccagc tctatgagct
     4141 gctaataact tttctttaac tcctcctact gctaagactc tacctcttaa agttatctct
     4201 cctgtcattg ctatatttcc aggaacaggt cttttagtaa gtgctgagat tactgcaaga
     4261 gccatagtta taccagctga tggtccatct tttggaacag caccttctgg aatgtgtata
     4321 tgaatatcat tcgtcttata aaattcaggg tctatatcaa acttgtccac tattgacctt
     4381 atataagaga tacctgtctt cgcagattcc ttcataacat ctcctaattt tccagttaat
     4441 acaatttctc ctttaccttt aagtacgttt acttctactt caagtgtaac tccaccaact
     4501 tctgtccacg caagaccatt tactaatcct atttgagggt taacttctgc aagttgatac
     4561 ttaaacatgt cttttccaag atatgtctct aggtcagact tatttataac aacctcttca
     4621 agagttgggt cttcaacgta ttttttggca acctttctac aaatcttacc aatagttctt
     4681 tctagtgttc ttactccagc ttctcttgta taatggttga ttatacttct taaagtttca
     4741 tcatctattt ttataaagct ttcttttaaa gcatgttctt taatctgttt tggaagtagg
     4801 tatttttttg ctatatttaa tttttcttct tctatatacc cagatacttc tattacttcc
     4861 attctatcta ataatggtct tggtatatta cctagactat ttgctgttgt aacaaatagt
     4921 atttttgata aatcaaaagg tatttctaag taatggtcca caaaatcctt attttgctct
     4981 gggtctaata cttctagcat tgctgaagct gggtcacctt tataatctgc tgccatctta
     5041 tctatctcat caaataagaa tacaggattt tttgtttggg cttcttttac cccattaatt
     5101 attcttcctg gaattgaacc tacataagtt cttctatgac ctcttatttc tgcttcatct
     5161 ctaactccac ccaaagatat tcttacaaat tttctattta atgctctggc gattgatttt
     5221 actatagatg tctttcctgt tcctggagga ccaactagac atattatagg ccctttaagt
     5281 gacttagcaa gagttcttat agctagatac tctagtattc tctccttaac tttttctaat
     5341 ccataatggt cttcatctaa tatatctttt gccttagtta tatctaattt atcttttgtc
     5401 tctttattcc atggcaatga gaatatagta tctaaatagt ttctacttac agatacatct
     5461 ggagacatag aagatatttt tgaaaattta tctatctctt tttctatttt ctctttagtt
     5521 gtcttaggtg cttttatttt tttaagcttt tctctatact catctgcttc agagtttata
     5581 tcttcttctt cacctaattc tttctgtata gcttttaatt gttctctaag ataatattct
     5641 ttttgaacct tattcatctg tttttttact cttaaagtta ttttcttttc tatttttaat
     5701 atatctattt cctctaataa tatagaataa ataagttcaa gtctttttct aatatcaaat
     5761 tcttctaata tttcttgttt ttggcttgat tttagatata tgtttgcagc tatcgtatct
     5821 ataaatctat ctacatcttc tatatcagct aaagatatta atatttctgg tgatacccta
     5881 tttccaatat ttatatactc ttcaaaggca tcaaatacat ttcttacaaa tgcttcaatt
     5941 tcaacttctg tttcactatc caaattatca ctatcaaata ctatttcttc tattactgct
     6001 ctgaaatacc catcttcttg ttctattttc tttactctac cccttgatac accttcaacc
     6061 aaaacacgaa ctgtatctcc tggtaatttt atcatttgct taactttaca gattgttccc
     6121 acatgataaa aatcctcttc tcctggctca tctacctctg cttctttctg agaagttaaa
     6181 aatataagtt cttcatccat catagcttgg tctaaagctt taagagatat ctctctacct
     6241 atatcaaaat ttaatatcat atatggaaat atagctaatc ctcttagagg aattaatggt
     6301 aattcatgat ctattttagt ataattttgt tccatcaaca ttactccttt tctagtctaa
     6361 ttgctttgct tttaatatta tatatttgaa caagttaatt ttcaacatta atgataaatt
     6421 cttagaatac tatcatacta tattatcttt ttattatgat ataatattaa tattagtata
     6481 cttagatgtt atttatcttt tggaggtttc tattatgttg aaattgtttt taaccttttt
     6541 aaaaattggt gcgtttacat ttggtggtgg atacgctatg gttcccctta tacaagatga
     6601 acttgtaaac aaacaaaaac ttttagagca ggaagaattt attgattatt tatccatagc
     6661 tcaaagttat cctggagtac tggcagttaa tatatctgta ttattaggat ataaacttta
     6721 tggtatacca ggagttctta tatgtacctt aggctctgct ttaccatcat tttgtatagt
     6781 cttagtctta tcgtatctat actttaaaaa ttcatcatct aaaattttag atggattttt
     6841 taaaggagtt gcgcctgttg taatagctct tatattgtat tcttttacga gcatgttcaa
     6901 gaaactacca aagtctaaaa ctaacatact actattaatt atagcagttt tatctgttgg
     6961 tgtattcaat ataagcccta tttatttaat aattggagga ggtgtatata gtctatgtca
     7021 gaaataatac tactcttctt aatattcctt aaaatagggg catttagttt tggtggcggt
     7081 tatgccatgc taccatttat ccagcaagaa tttatatcta aatatcactt tataactaat
     7141 caagagtttt tggatttact tgctatatca caatctacac caggtcctgt agcaataaat
     7201 agtgctacat ttgtaggatt taagactgct ggcttctttg gttctctagc tgcaactatt
     7261 ggtgttacaa tttttgcatt aataggtcta actattatat caaagttatt ttctaaattt
     7321 aaaaataata aaaaggtaga aaacttattg acagttctta gaccaattac tctaggattc
     7381 atattatcag cagctttttc atctgccaaa gaagttaatt gggacttata tgggataatt
     7441 gcatttatag catcatttta tcttttatat aaaggaaaaa ttggttccat tgcaattgta
     7501 ttcgtctttg gtattctagg tatattactc acatttttat aataatgata cctattttat
     7561 atttagcata ttaacataaa aaaaggagct tttaaagctc cttttcatca tcttatgcac
     7621 tttcctcttg atccttaagt actatgatag gatttactga gtcttcgttc acagatttct
     7681 ctgttactat aaccttcttt atattatctc tagatggtac ttcaaacata gtttccatca
     7741 ttacactttc aactatactt ctaagtcctc tagctcctgt atttctttct atggcctttt
     7801 tagcgattgc acgaagagca ccttcttcaa attctagctc aacatcatct aactccaata
     7861 actttttata ctgttttact agagcatttt taggttcctg taatatctgc attaatgcat
     7921 cttcatccaa caactctaaa gtagctaaaa ctggtattct tcctataaac tctggtatta
     7981 taccatattt taataaatcc tctggaagta ctttttcata cagcttccct aaatctaatt
     8041 cttttttact ttctatttta gctccaaaac ctagtgtttt atctccacca cgtttttgaa
     8101 ttatcttctc taatccatca aaagctccac ctaatataaa taatacatta gtagtatcta
     8161 tctttaaaaa ttcttgatga ggatgttttc ttcctccttg tggtggaaca tttgcaacag
     8221 taccttctaa tatttttaga agtgcttgtt gaacaccttc accactaaca tctctagtta
     8281 tagatgggtt ttcagatttt cttgcaatct tatctatctc atctatatat attatacctc
     8341 tttcagcttt ttctatatca aaatcagcag cttgaataag ttttaaaagt atattttcaa
     8401 cgtcttctcc aacatatcca gcttcagtca atgatgtagc atctgccatt gcaaaaggta
     8461 catttagagt tcttgctaga gtttgagcaa gtaatgtctt tccagaacct gtaggtccta
     8521 ataaaagtat attactcttt tgaatttcta tatccttact actagatttt ttactgtata
     8581 ttctcttata atgattataa accgcaactg ataaagcttt tttagctttt tcttgaccta
     8641 ttacataatc atttagaatc tccatcattt cctttggttt tggtaaagat gtagtatctt
     8701 catctatagt atcttctatt tcttcctgaa taatttcgtc acaaagttca acacactcgt
     8761 cacaaatata tacatttgga cctgcaatta atcttcttac ttgatcttga tttttaccgc
     8821 agaatgaaca ctttaattgt cgtttttctt catatttaga catattaaca cctctctcta
     8881 tctacggtct ctttgtaaat acttcatcta ttaaaccata ttctttggct tctagtgcac
     8941 tcatgaagtt atcacgttca gtatccattt taattttttc taatggctga ccagttcttt
     9001 cagataatat ctcatttaaa gtttctttta tttttaaaat tctctttgca tgaatttcta
     9061 tatctgttgc ttgaccttga gctccaccta atggttgatg tatcattatt tcactatttg
     9121 gtagtgctag tctttttcct tttgctcctg ctgccaacaa gaatgctccc atagaagcag
     9181 ccatacctat acatatagta cttacatctg gtttgatgta ttgcatagta tcatatatag
     9241 ccattccaga agttatactt cctcctggag aatttatata taaatgtata tctttgtctg
     9301 ggtcttcagc ttctaaaaat aatagctgtg ctactataag ccctgctgtg gcatcgttaa
     9361 cttgatctcc taagaagatt attctgtctt ttaatagtct agaaaaaata tcataagatc
     9421 tttctcctct tcctgtttgt tcaacaacta caggtactaa tgccatggtt tcataccccc
     9481 tttattctat ataatttaca gtagcttttt ttaagctact gtaaattatt atacgcattt
     9541 aactaaatta tatttgcaca tcattatata ctaaacattt cataacgtca actatattag
     9601 ttagcgttta ttttattaag ctattgttgc attatcaact aataaatcta ttgtttttct
     9661 tatttttatt tgacctttta tatcttcttt gtcagcatct cttaaagaag cttctatttt
     9721 ttctacttcc atattgtaag cagaagccat tttttctaac tcagccttaa attcttcttc
     9781 tgttgcttca attccttcag ctttagttat tgcttctaat actaaagaag acttaactaa
     9841 ttttttagca tcttcttttc tctcttctct taattcttcc atagttctac cagtcatctc
     9901 tagtaattgt tgtaatccaa atccttgata ttgtaattgg tagttaagtt catttaacat
     9961 attatctatt tgatgttgta tcataacttc tggtatttct atttctgtat tttcagcaac
    10021 tttttctact actgaatttc tagtttctgc atctgctcta tttttagctt cttcttctaa
    10081 cttagctctt acatctgctt ttaattcatc taaagaatca aattcactag tatctttagc
    10141 aaattcatca tcaagtgcag ataattcttt tacttttaca tcatttattt ttacattaaa
    10201 tacaactggt tttccagcta aatcttgtga gtgatattct tctgggaaag taacattaac
    10261 ttctacttct tcaccagctt ttttacctac taattgttct tcaaatcctg gtataaatgt
    10321 gtttgaacct ataactaagt tataattttc acctttacca ccatcaaaag ctacaccatt
    10381 ttcaaaacct tcgaagtcta ttatagctgt atctccatct tctaaagctt tgtcttctac
    10441 acttactaat ctggcatttt tttcaaccat ttcttgtagt ttagcatcaa cattttcgtc
    10501 acttacagta ttctcaacct tagctatttc tatacccttg tagtttccta attcaaactc
    10561 tggtttaact tctacattaa gaaccataac caacccattg tctttactta tttcttctat
    10621 atctaaatct ggattgtcta ttgggtctat gtctaattcc ttgatagctt ctggataaac
    10681 ttctgggaat aatatttcta tagcatcatt gtagaatata ccttttccgt attgagtctc
    10741 tataacaatt cttggagctt taccttttct aaatccaggt atattgtatt tatttctgct
    10801 tttattataa gccttattta ctgctgcttc aaatttattg ttatctactg ttatttttaa
    10861 agtaacttta ttaccctctt ttttaattag ttctgctttc atccttctaa tcctcctagt
    10921 gatatttatg taatacccga taacggtatt ataattcatt ctatttataa ttcaaccact
    10981 taattatatc acataagttt ttttatttac aagtgttcca ttaatatcgt tgtacttatt
    11041 tgataaaatt taattttttg agcgtttttt gccatttttt tagataaata cacctttata
    11101 tgcgtatttc ttcaattttt atgtcatctt cttctatatc cataatgaca aaacttttat
    11161 acgatactcc tcttggaagt gaagtgcttc caggatttat gtaaagtaca ccatctttaa
    11221 tttctctaaa tggggtatgg gtatggccaa atataactat atcagcgtca acttcagttg
    11281 cctttttttc taacatatga gttccatatt ttacgccata cttatctcca tggcataaga
    11341 atatggtttt atttgccact tcaaagacaa cttcttcctc aacagcatca aagtcacaat
    11401 tacctttaac tgcaataata cctacattag tcatgctatg aatatatctt gaatcagtaa
    11461 agttatctcc tgcatgaatt attaggtcac attctttcaa atatggtatt gttttttcca
    11521 tatttttaat catcatatgt gtgtcgctta ctatgcctat tttcattact tcactaccct
    11581 ttttataaac tcttgtttca taagttttag tgcgtttgct ctatgactga ttgaattttt
    11641 tataacacta ggtatttcac caaaagtctt atcatatcct tttactataa ataagctatc
    11701 atatccaaaa ccatttttac ctttttcttc aaatcctatc attccttcac aaattcctct
    11761 tactacaaat tcttttccat caggaaaaac tactgctatt gcagagacga atcttgcatt
    11821 tcttttgctc attggtatat tttgcatagc ttttaacagt ttttttctat tttcttcatc
    11881 tgttgcatgt tctcctgcat atctagctga ataaacacca ggttttttgc ctaaagcatc
    11941 tacttctaag ccagaatcat ctgatattgc tataagttta gtcttttttg ctattgttct
    12001 agcttttatc aacgcattat gctcaaatgt ttttccatct tctactatct ctatacctgc
    12061 taaatcaaca tcttttaaag agtacacttt atattcaaaa tcttttaaga tttcacctat
    12121 ttcttctagc ttatgagcat tattagtagc aatcactact tcattaccat aatccatacc
    12181 caatatctca tctgatattt cacctaaagc ttctctttga agccttatta attctttatt
    12241 ccctttttca cctaactcta acaataaatt taaatctgct ctcgaaaaag gtttctcttc
    12301 tcctgtcccc tgtacttcta caaactcaca cttatcagtc attattatat tcatatctac
    12361 ctgtgcttta gaatcttctt cataacataa atctagcatg tgtattccat ttacaatacc
    12421 aacactaatt gcagatacaa aatttgttat tggcatctct tttattgtct ttgctttata
    12481 taatttatat attgcttctg caactgctac aaatgcccct gttatagatg cagttctagt
    12541 tccaccatct gcctgaatca catcacaatc tacccaaata gttctttctc ctaatttatt
    12601 caagtctaca acagacctta ttgctcttcc tattaacctc tgtatttctt gtgttcttcc
    12661 atcaattttt cccctagatg catctcttat ctttctttgt tgtgttgctc ttggtagcat
    12721 agaatattca gcatttatcc atccagtacc actatttctt aaaaaaggag gaactttttc
    12781 ttccactgat gcagtgcata taacctttgt ctctcccatt tctataagaa cagaaccttc
    12841 tgcatacttt gtataatctc tagttatctt tacaggtctt atctgttcgt tacttctatc
    12901 ataacttctg gtcatttttt atatcctttc tcatattttt tattttttat aaatttattt
    12961 ttataatttt taattttgtc aaatattaat tattgtcatt tttcgtaatc ttattaactt
    13021 actgaactat tctatcatta attatagtag ttttctacta aattaagcga ctttttaact
    13081 ctctaataca gcttttactt catctttcga tgataacttt aataattcta aaaatctctc
    13141 ttcatctata actttaattc ctaaatcatt tgctttagta agtttagaac cagcttcttc
    13201 acctgccaaa acaaatgttg tcgatttact aacactagat gtagcttttc cacctctttt
    13261 ttctatcata tcttttgcat catttctttt taatgtagga agtgtgccag tcaatacgat
    13321 tttcatccct tcaaatattt tttctacttt ttcacctgta ttatccaatg attttgtatt
    13381 tactcctaca ttttttaact tgtttataac gttcatattt ttgtcttcct taaaaaactc
    13441 gacaacgctg tttgccatta tacttccaaa ttcctctaat tcttctagtt gctcactagt
    13501 tgcagaaatt acttcatcta aatccttaaa gttatatgca agtatcttag ctgctttaac
    13561 ccctataaat ttaataccta atccatttat aagtctccat aaatcatttt cttttgattt
    13621 ttcaattgaa tttattaaat tgtcagcaga tttttcgccc attctctcta ggtcgactac
    13681 atcttccttt tttatataat ataaatcaga tacatcttga ataatttttt catttaatag
    13741 cagtgtaatt atagattctc ccaatccatc tatattcatt gcatcccttg acacaaagtg
    13801 tataattccc cttcttattt gggctgggca agacatattg atacatttta cagcagcctc
    13861 tccttctaat cttattgttg gctctgaaca aactggacac ttttctggca ttttaaaatc
    13921 tatttcttct ccagtacgtt cctcttttat aacttctaaa acttgaggta ttatgtcccc
    13981 tgctttttga actaatactg tatcatttat ttttatatct ttttctctta tataatcttc
    14041 attgtgaagt gtagctctac ttacagtagt acctgcaagt ctgactggtt caagaattgc
    14101 tgaaggtgtt attgtaccag ttctcccaac ttctactatt atatctacta ttttagtttt
    14161 cttcttttct gctgggaatt tatatgcaat tgcccatcta ggacttttgg ctgtatatcc
    14221 catttcgtct ctttgtttta tattgtttac ttttattacc ataccatcta tttcgtagtc
    14281 taaattaccc ctgttatctg tccagtattc aatatgctca ataacttcat ctatactttt
    14341 acaaacttta taactctgac ttactgaaaa tcctagtttt tctaagtact ctagtgattc
    14401 actgtgactt tctaaatcgt attcttctat attttccata ttaaatacaa atatatctaa
    14461 tggtctcttt gctgttagtt ttgggtctaa ttgtctcaat gaaccagctg caagatttct
    14521 tggattagca aataatggct gttcttgttc ttcttgttgt tcatttactt ttctaaaatt
    14581 atcttttgat atatatactt ctcctctaac tattagttct tttttatcat ttatcttaag
    14641 aggtatgctt tttactgtca ttaagttttg agaaatatct tctcctacaa ctccatctcc
    14701 tctagttgct cctttttcaa actctccatt gttataagtt agacctacag aaagaccatc
    14761 tattttaaat tcaacaacat attcaacatt ttctcccaca tattcttgaa ctctcttatc
    14821 aaaatctctc aaatcttgct ctgaaaaagc atttgataaa ctcaacatag gagttttatg
    14881 tacaatttga ttgaacttat ctaatggctt acctcctacc ctatttgttg gagaatcaat
    14941 tctctttagt tctggatgtt cttcttctaa agaaattaac tccttcatta acttatcgta
    15001 ttcataatct gatatttctg ggctatcttg attataatac ctttcattat ggtagtttat
    15061 tgtctctata agtttattaa tttcatcttt tacgctcact ttttgcacct cttcatattt
    15121 ttaaaattaa attattttga aatttcccta taattaatta tctcctattt atatattttt
    15181 tagtatatca cttttttggc tttaaattta ttacaaaaaa aactcttatt agtatcttta
    15241 atacgactaa taagagtttt tatttttatc aaaatttttt atttttaata gcctcttgat
    15301 atgtctacaa cattatctac aggtttattc tccattatat agtgtttcaa attattataa
    15361 accatattaa atgttctctc tttgttttta tctgacaccc aagaattatg aggagttact
    15421 atgacatttt cgcactccca caacttattg tccttgttta aaggttcact ttcaaatacg
    15481 tctaatgcta cccctctaaa tttctctaca cattcttcta aatctttctg attaactgta
    15541 tttcctctac ctacatttat aaatacagaa ccatctttca taagtttaaa catatctttg
    15601 tttactattc catctgtact ttttgtagaa ggcattgcta caactactat atcacattct
    15661 tcaaatacaa tattcatatc ctcagtagca aaacacttat caaagtattt tttactactt
    15721 ccatcagtat tgacacccca tatttctaca ccaaaagctt ttaatctttt tgctgcttct
    15781 gaagatatag tacctgttcc taaaaatcca actctttgtc catacaattc tgatacagaa
    15841 aaatccatct tccattcttt attttgttgt tgtttaaaaa attttgcact atttttatat
    15901 atttctaaaa tatacaatac aatactttca ccaataggta cactatatcc gcctctatta
    15961 ttagctatct taatatttct cttcaatacc tcttcttttg gaagttggtc tatacctaca
    16021 ctagttgtct gtatatattg taaatttttc atcttagaaa tatctaatct ctcaaatgga
    16081 ttgtatgtaa ctagtacatc tgctgtattt acatcctcat tattttcaat actatcttca
    16141 gagtaatgta tgacatcata gcctaattct cttattttat caaatttttc ttttccatag
    16201 tttattgtaa ataaaacttt catctcaaat ctcctccatc tacccacact taaaatttta
    16261 tattctaaaa tataatatga tactcaatgt acattagtta aaattactat aagatatctt
    16321 aaatttactt atgttcaatt gtttttccta aagacatagc attacgttct tgaactaaat
    16381 tttctattaa attattcaat gtccaatcaa tagatgtctc tgttaccata gctgtaaaag
    16441 gtctatttat tctcactttt tttagccctt ctaaaaatgc atttacttgt gtgcttggag
    16501 tgttattaaa tattacagac ttactcttaa tcccattttc acattcattt gatatatttc
    16561 catctaaaat gtctcttatt atattctctc catttttatc acttaagatg atatggtctc
    16621 ttattccagc aaagttactt acatttttaa ttaatgataa ttctttttta ttaaagttta
    16681 caagcataat acacttcctg ttgtgtattt tatctaggtt attattattt attttttcaa
    16741 atgacatttt atccatcctt tcatttattt atatacttta atttacccca tacatttaaa
    16801 ataatcaatt tttatagtta atttttatat taatacccaa attatattta aatagttagg
    16861 ctatccaaaa ctaatatata aaattagaat aattagatta atatctacaa atcataacct
    16921 aaaggagaga taccatgaat acattactaa acaataaatt tagcattaaa aacttttcaa
    16981 atgcttgggt tatagtactt ggttgtatgt taatacaagc tgttccattt agtattgtag
    17041 caagtataca gcctcaattt ataagttatg ttgtagaagg agaaggcttt agtttagctt
    17101 ctttttctct aatattcact attggtacta ttgcctcagc aattgcatca ccttttatag
    17161 gtgtattatt ttctaaagtc aatattaaga tactatattt attaggttgt ataatttcta
    17221 gtattgcttt tgctactttt tctatatgta ctgaactatg gcaattttac actgtatcat
    17281 gtattgttca aattggcgct acaattatat ctgctatagg aatacctgtt ttaataagtg
    17341 cttggtttga tgaagaaagc aaaggaaaag ctttgggctt tgcttttgct ggtggttcta
    17401 ttggtagtat atttctacaa ccacttgcag ttcgcttatt agcaactcaa ggatacagtc
    17461 gttcttatct aatatttgga attatagcat taatagttgg tatacctgta atattattca
    17521 tactcaggat gcctaaagat attaatgaaa aggtaaaatc taaaggtaaa aaagagaaag
    17581 catctgatac aatttctcct tcagtatggg gttatacatt aaaagaagct aaaaaaatta
    17641 aatatttttg gattatgact attggattta tatttgttgg tatatatgtt tcatcattat
    17701 ccgtacaata tccaacttat cttagaaatt atctaaatcc tattctaata ggaacagtag
    17761 gctccacatt tgctttattt gctctcattg gtaatttatt tggaggatta ttgtttgata
    17821 aattcggtca aacaaagcca cttattatat cttttgtatt ggttcttata gcaactttat
    17881 cattactatt ggtagataaa tcttctactt ttgcatttgt atttgccatt ttgaaaggtc
    17941 tttctgtatt tgcttatatg attggtcctg cttatttaac tggctctttc tttggtcaaa
    18001 aggagtttgg tagcatatat ggaatcgtac aattaatttt tgcagtggga tttgcattag
    18061 gttcaacatt atttggattg ttagttgata attttggata cagattttct tggtctatta
    18121 taattttatc agttataatt gcctatactt tacttattat aacttctata ggaatgaaaa
    18181 aattgaataa agaaagatta gataaactaa tgcaataatt gatttttaga tatataaata
    18241 ttataataaa aaagatggag cttttaatca taaataaaac tccatctttt ttatattaca
    18301 atatagctaa ttttctctta tttttaactt tactatctat atcctcatcc ttaactatag
    18361 taaatgcctt agttggacaa acatttacac atgcaggccc ttctggcgat tctatacaaa
    18421 aatcacactt atttgctgtc attctttctc taaagcaaag ctttccttca tctacatctt
    18481 ttaatgtaac tatgtctatt gcacctatag gacatgctaa catacaagtc ttacatccta
    18541 tacaattttc ttgatttatt tttattgtgt taccaaccct aactatacca ccatttggac
    18601 atgcctttgc acaaggagca tcttcacaat gtctacactg tattggagca cttacttctt
    18661 ttgtctttat gacagaaagt tttggattaa aatttatttc atctggattt tggtaaaaaa
    18721 tatcttcatc tgaatgtgca actacacatc ctatcataca agttctacat cctatgcatt
    18781 tatcagggtc tgctataaca aaatagttca tcacttagcc acttcctttc ctatacaata
    18841 tgtagtgtgt aatagatgat gagatttttc tcctaatggt tctaccagat attcatcata
    18901 cagtttttta atatcaggat tctcatgaga ttttctaatt gctaaatgtt catcatgcac
    18961 gtatgttgct tttgttctat tttcataagc caattcctta tattcttcta gtaaaatttt
    19021 tggttgacct ccaccactta cacacccaac aggacaagtc ataacttcaa taaaatctac
    19081 atctaatttt cctttttcca agtcttcaag cactggtatt acatttttta atccacttac
    19141 tactccaact cttaagtcta agtctcctat tttaatagtt gactttctaa aaccattttc
    19201 tcctcttact tgttttactt ctacatcagg aatactttct ccagttataa gctcatatcc
    19261 tgttcttata gcagcttcca taaccccacc agtaacacca aagatagttc cagctccact
    19321 ataactacct aaaggactat caaatttttc ttctcttaaa tttttaaaat caattcccat
    19381 gtcttttatt aaataagcta gttctctagt agttaatact acatctacat ctctatatcc
    19441 actagaatcc atttcttctc tatcacattc atatttttta catgtacatg gcattataga
    19501 aacactaaat atctttgatg catctacatt atttatctct gcaccataag ttttaaatat
    19561 tgcaccaccc atctgctgag gtgatttaca agaagataaa tgattagtta atttaggata
    19621 atttttttcc atgaatttta cccaagctgg acaacaagat gtaaacattg gaagagttcc
    19681 accttctgtc actcttttaa ttaattcact accttcttcc attatagtta aatctgctgc
    19741 aaaattagta tcgtagactc tatcaaaatt taaagctcta agtgctgctg ccattttccc
    19801 agcacctaag tatcctaaat aatatccaaa atcttctcct aaagcaactc taactgctgg
    19861 tgcacattgg accatagtga atacatttgg gtcacttaaa gctttcttca ccttatctat
    19921 atcacataca ttgtatgctg caaatatagg ttcatttata gactctggta tctctctttc
    19981 ttgttttttc tcttgtaaaa actcaaatcc ttcatctata actgaggcat aagatttaca
    20041 agtttgaaca cattgaccgc acataacaca tctatctaaa tttatctctt gtggctttcc
    20101 ctcttcacct tcaatagcat ttactggaca gacctttgaa cattctctac atccagtaca
    20161 taaatcctta tctatattta ttatcataaa cgtcacctac ccctgtatcc tttatttatt
    20221 tgttaacaat aaactaagtg cagcttgtct attcttagct tttttgtcat ctattggagt
    20281 aacaagtttt aatgcttcct gaggacatgc acttatacaa gctatttttt cattgtcttt
    20341 acataagtca catttataag ctatcttctt attttcatct aaaattactt gttctacttc
    20401 tttaccatct tcatattgtg gaagtaagtc tatagctcca aaaggacatg ctagcagaca
    20461 agttttacat cctatacact tttcttcatt tacagacata atattgtttt ctttaactat
    20521 agctttttgt ggacatgagt ttaaacaagg agcatcttca caatgtctac actgtattgg
    20581 catacagaaa ttgtcacctt ttactaagta aagtctaggt ataacaggta tatcaactgt
    20641 tccaactgta taacccacat tattatgctg attatgcatt gtaaaacaag ctacttcaca
    20701 agctctacaa cctgtacatt tgtttgggtc tgctattaca aaagagccta ttttattttt
    20761 catattatcc cacctttcaa tttgtatatt tccaaccctc tagttatttt catcatatgt
    20821 ctacttactt tcatcatata tatttatata tttcactatt ttctcagtac ttatatttcg
    20881 tttacatttt ttacacaaat tgtatgtgtt attatacacc ttatcagtat catcaattaa
    20941 tactccacag atttcacact ttgaacattt ttgttcttct tttttagtac acatatactt
    21001 attctcactt tttgataact ctctgtggat ttgcataaat attcatccgt cttcctctta
    21061 caaatccaat aagcgttata tttaattttt tagaaagctc tattgataaa ttagttggtg
    21121 ctgattttga tactacaata ggaatttgag tcatagctgc ttttaatatc atttccaatg
    21181 atattcttcc acttacagcc acaattttat cttttaatgt tatgtcattt aaaacacaaa
    21241 atccaatagc cttatccatt gcattatgcc ttgctacatc ttcacaagtg actatttttt
    21301 tatcaaaatt aaatacagat acgctatgta ccccaccagt atttttaaat aattctgatg
    21361 aatttaaatt tttatccatt atactgtaaa tcgtatcata atttattttt atattgctct
    21421 ttacaggagc acatgttata tagtctagtg aatttaaaaa tataatttgc tcatcttgat
    21481 ttttttgttt ctcttgaata gttacttttg caaaacgttc actctcattt atctcaaatt
    21541 ttaataagct ttttttgttt tctatgtatc cttttgtttt taagaatcct actataagtt
    21601 cctctaaatt atatggggta cataaaaatg tatttatata tttatcattt aaaataaaac
    21661 taagtggata ttctgcaatt atttcttcat tatctattga tacttcatgt tcatttattt
    21721 tgcttacttc tacattaaaa gaatttaaat atttgtagtc cactagacta taactatttt
    21781 caaaaacttt atccttacta ttttcttgta atttagtttt tgagtatata ttgttcataa
    21841 aatattagcc tttctgcctt ttataaatct taaatctaat aaatcattag atatttgtag
    21901 ctgtcatttt gtctttcaaa ctttgatact cttctcttac acatttttct gctaattctt
    21961 ggtcttcaag tttttcaagt ttaacagcac aatatttata ctctggtgtt ttagatatag
    22021 gatctaaatt tgctattgta agttcattgc aagctccaac ccaccactga taagtcatat
    22081 atgttgcacc ttccttaact ctatcagtaa ctgttgctct tgttattaca cttcctcttc
    22141 ttgaactaat tctaactaat tcatcatctt caattcctaa tttttcagca tcattagaat
    22201 ttatttgaac acgtcctggt tcatcttcta atgagctaag tgttctacaa ttaccagtca
    22261 ttgttcttac tgaatagtgt ccaacttctc ttacagtaca taaactaaat gggtattcat
    22321 catcttcaac ttccataggg ggtctccatt cagcagcgaa caaattacct ttaccattag
    22381 gagtagaaaa cttttgacct tcatacagat acatagttcc tttatcttcc atactttcac
    22441 tcttacaagg ccattgaacg catccttgag tttctatctt ttcataagta gcacctaaga
    22501 aacttggaca taattgtcta agttcgtccc atatttcctt agtattttta taattcatag
    22561 gatatcccat tgctgtagaa atttcgctta taatttgcca gtctggtttt acatctcctt
    22621 gtggctctat agcttttctc atcaattgga atcctctatc tgcacatgta tatactccct
    22681 catgttctcc ccaagaagta gctggaagta taacatcagc gtgaagtgct gtcttattca
    22741 taaatatatc ttgaacaatt acaaattcca attcatctaa tgcttctcta acttctgata
    22801 catctgggtc actttgcact gggtcttctc caaatatata ataagcttta agctttttct
    22861 cttttagtac taagtctgga acttgagtta aactataacc attattagga gataatttta
    22921 ccccccaagc tttttcaaac ttttctctta tcttatcatc agttacattt tgatagccag
    22981 gatatacatt aggaagagct cccatatcac aagctccttg aacattattt tgtccacgta
    23041 caggtcctat tccaacgctt tctctaccaa aattacctgt aagtagagca attgatgcaa
    23101 gtcccttaac tacatcaaca gcttgaccaa attgacagac acccattcca taaagtatca
    23161 tagctttttt accttttgca tattctctca tagcttttct tataagctct tctgggattc
    23221 ctgtaatctt ttcagcatat ttagctgtat atggtttaac aatttcttta tactcatcaa
    23281 atccttgtgt atgattttga acaaattctt tattgtaaag accttcttct attaatacat
    23341 ttccaaaagc atttactaaa accatgtttg ttccaccttt tataggtaga tgtatatcag
    23401 caattcttgc agattcagtt actctagggt cagtaacaat taatttagca ccattttttt
    23461 tagcttttac tattctattt gccactattg gatgtgaatc tgctccatta tatccaaata
    23521 taaataaaac atctgcattt tctatctctg gtatagaatt agacattgca ccactaccta
    23581 atgagtaagc cagaccggct acagatggtg catgtcagac acgagcacaa tggtcaacat
    23641 tattagttcc tattgtagct ctcataaatt tttgcattat atagtttgct tcattaccag
    23701 gtcctcttgc agaacctgtt cccataatag aatcaggtcc atatttttct ttaatttcat
    23761 ttagtcttga agctgtatag tttatagcct catcccattc aacctcttct agtacacctt
    23821 gttttcttat cattggcttt tttagcctag gtgttagaat tttagggtca tttagaaaat
    23881 cccatccata acgacctttt aaacacaaac tttcttgatt tgttctacca ttagccggtt
    23941 cagctcttaa tatcttatta tcttttacta ctagatataa ttggcatcca gctccacagt
    24001 atggacaaac tgttaaaatt tttttctcca tacaattccc cccttagatt tatcccctga
    24061 taagtaaaat ttatcttaaa gataatgagt gtttttataa tatgcaaaat cataaatatt
    24121 ttgcaatact atctattaaa ttcaacaatt ttctcacaat tcctcttaaa tttcaaaaaa
    24181 aatatgtcat aataatcttt atatttcaac catttaatta aaatattcta gttcttatga
    24241 catatttttt aatatactca atatatattc tttaaactta tttagttttc acttaaaaca
    24301 atatttctgc caattctatt tttcattcta tagtgagtaa ttacagtaaa tattccactt
    24361 ccaatcagaa tcaaatagta cataaatcca ccatataaaa atactgcata tcccatcaaa
    24421 ggttgaggga atactgattt gaatatagtg aaaaaggcta attcattagc tcctacattc
    24481 ccaggtgttg gtattggaga tattgccata tataaaaatg cttgtaatgt cagtatataa
    24541 atatagctat aaccttgtaa attaaatgct ttatatatcc aaaatgaaac actaaagtaa
    24601 gcacttaatt gaataaaagt tactattata gttgaaatta ataccttttt attctttgca
    24661 aaaaaattta ttgctttact ataatcatct ataaaacttt ctatggattc aatcttatta
    24721 tctaaaaatt ttaaaaactt aatttttgat aaatgcttta tagtaattct cataaaatat
    24781 tttatttttt taggatttaa aataactaat attatcataa ttaagacaaa tgaattaatg
    24841 gctatcccta gaaacactag aggcataatc attttcattt gtttttctaa catagcaaaa
    24901 ttcattaaaa caagtattgt acaataaaaa gtaactacta tttggaagat aatagactta
    24961 ttggtaacaa ctgcacttgc cttactaagt ggcattttac attttttaag tacatatatc
    25021 tgtactggtt ggcttcctga agcaaaagga gttattaaat tataataaaa tcccataatg
    25081 gctagcttaa aaccaataaa tctaatatta actttgtgag tgctttctat aattatcttc
    25141 attactactc cttcaagcat tatgtgacac attattgcca aagcacctat gaataaaaac
    25201 ttcttatcta ccataaccat aacatttgat agcattttaa tatccaatgt tttaaacact
    25261 aggtaaattg ttatacctat taacaaaaat aaaaatgtat actgtagtaa actctttcta
    25321 atattcttta gtctatccat tagaaataac catcctttgc tcaagattta tactaaatac
    25381 tatattatta gtatacacta tgaatcttaa tattttataa caaaatacct tacaattttg
    25441 tcatttacaa aaaatattaa tctataaatt aaattttaat atatagatta atattttaca
    25501 aacattttgt agatatatat aatattttca ctaaacaaca cttatactta ttacacccat
    25561 ttatacccta tcccccatac agtttctatt gtataaatat catattgttt taatttgctt
    25621 cgtatatttt ttatatgctc tgtgatagta cttatatcac tttctccatc aaaaccatat
    25681 accgactcat atattctttc ttttgaaaat acttgtccct tattttttgc tagaaattca
    25741 catattagat attcactttt agtaaaatta atttttttac catcaattga aacttctttg
    25801 cctaaaaagt taaattgaac atttgagata gttaataaat tctttttttc tctactttct
    25861 cgccttaaat gagcagttat cctagccctt aattctccta caccaaaagg tttggttata
    25921 tagtcatctg cacctatacc aagaccgtac ataatatcac tttccatatt ttttgctgtc
    25981 aaaaacagta ttggacaatc aaccttattt cttatagttt tacatacttc aaaaccatcc
    26041 atcttaggca tcataacatc taatattatt agattaaaat aattaaaatc catgttcata
    26101 gccttttcta catcactaat tacctttact tcatgtccat ctcttttcaa cacattttca
    26161 atcaacttta acatatctaa ctcatcatct actactaata tcttagacat aataactcct
    26221 ccatttatcc atctaatagc attctaattt atatatttaa taatatcttt aaaattcaca
    26281 ttaaccataa tactctcata ttatagctta caataattaa ttactatttt acaaataaaa
    26341 tattttagat acaaaaactt tatatttcaa aaactttata ttcattttac acgtatttac
    26401 ttaaatataa aaatttattt aacaatttat tattgtaata ttttgaatat ataataaaat
    26461 taataataag aaaacttttt ataaatatac ttttaattgt ttaaattgga ggaaaaaatt
    26521 atggatgaaa agataacaaa aaataattat aatgttccat ttgactatgt tcgaaaaaaa
    26581 ctcaaagaat tagaccctca tacaattagt aatttaagta atataccttt caataatgat
    26641 ttaaatagat tcacactcaa tttttttgat agacaatata ttatagacta tccaagtgga
    26701 gaagtttgga atagtaatgg tagcctttgc aataactttg agcttaaaat cttgatagtt
    26761 agatatttaa tcaatgctaa aggtatccct agaactaata aatatgtaac ttttaaagaa
    26821 ataccaggag gcaatgttta ttatccaaat tttttaaaca ggactctata taggctttca
    26881 gaagttttta catctcaaat agacaaatat aagttagtta tggagaaaat tggagctgaa
    26941 aaagtaaaca tgggagattt agcttatagg tttacatata tgggtaatac atctatggta
    27001 tttatacttt ggtttggaga tgatgaattt cctccaaatt caaatatatt atttgattct
    27061 aatatagttt attacttcaa tgcagaagat ttagcagttg ttcctgatac agctataaat
    27121 gcaattttaa ataaaataaa tcaattataa atataaataa tattataata aagtttttga
    27181 tagatttaat agaatataat atgaaatgga gtaaaaaatg aatgtagaaa atcaatcttt
    27241 atcagatatt gaaataatat tatcacacag gtatgataat ggtgcagact attggactac
    27301 acctgataaa cgtttaataa aaggttctcc tttctctact cttgatagtg tactttatct
    27361 attagaatta ggtgtgaacc ctgatgatac tttacttaaa gaatgtgctg acctgatatt
    27421 tagcacatgg cgagaagatg gacgtttcaa actatatcca aatggaggta tttacccttg
    27481 tcatactgca aatgctgcta acgtactctg tcatatggga tatgcatctg acattagaat
    27541 gcaaaaaacg tttcaacatt ttttagatac acagtatact gatggtgggt ggcgttgcaa
    27601 taaatttagt tttggtcgag gtccagaaac tgaatactca aatccactac caactcttaa
    27661 tatattaaat gcattccgtt ttagtaatta tctaaataaa gaatcaaaac ttgacaaagc
    27721 tgtagatttt ctacttgacc attggactat taaaaaacca atcggccctt gtcactatgg
    27781 aattggcaca ttatttatgc aagttgaata tcctttccgt aattataacc tattccttta
    27841 tgtctacgtt ttatcatttt ataatcgtgc aaaaaaggat aatagatttt tagaagcatt
    27901 gaagatactt gaatctaaaa tgataggtaa taaaattgtc gttgaacgtg tagttccaaa
    27961 actatccaaa ttatcttttt gtaaaaaagg cgagtcaagt gaaatagcca caacacacta
    28021 ttacgaaata ttaaaaaacc tagaaaaata atagttatat agataaaaca tattaaaaaa
    28081 tag
//
LOCUS       DAEMHW010000036        27427 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEMHW010000036 DAEMHW010000000
VERSION     DAEMHW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.036"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..769
                     /locus_tag="KPK67_001323"
     CDS             <1..769
                     /locus_tag="KPK67_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBG2458316.1"
                     /translation="DISIVAYIFYKIFMFIKDTRAEQVFKGIIFLLLATQLSNTFKLH
                     TVYWISLKALDYGVIAALIIFQPEFRAGLEHIGRAKFNLFGKNVNTSEETLNRNIEEI
                     VEALYSLSRQKIGALIIMERETRISDIINTGTIIDAEISRQLLINIFIPNTPLHDGAV
                     VIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGIGVSEVSDCITLIVSEETGGVSIAK
                     AGKLYRDISRERMMNILRSNLKTNTETRSFFKGGIFK"
     gene            766..1947
                     /locus_tag="KPK67_001324"
     CDS             766..1947
                     /locus_tag="KPK67_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458317.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDGLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            2251..3153
                     /gene="ptb"
                     /locus_tag="KPK67_001325"
     CDS             2251..3153
                     /gene="ptb"
                     /locus_tag="KPK67_001325"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBG2458318.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            3175..4254
                     /gene="buk"
                     /locus_tag="KPK67_001326"
     CDS             3175..4254
                     /gene="buk"
                     /locus_tag="KPK67_001326"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG2458319.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVRVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            4244..4921
                     /locus_tag="KPK67_001327"
     CDS             4244..4921
                     /locus_tag="KPK67_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2458320.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVVCLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            4964..5179
                     /locus_tag="KPK67_001328"
     CDS             4964..5179
                     /locus_tag="KPK67_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2458321.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            5208..6287
                     /locus_tag="KPK67_001329"
     CDS             5208..6287
                     /locus_tag="KPK67_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG2458322.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            6287..7039
                     /locus_tag="KPK67_001330"
     CDS             6287..7039
                     /locus_tag="KPK67_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG2458323.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            7040..7597
                     /locus_tag="KPK67_001331"
     CDS             7040..7597
                     /locus_tag="KPK67_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG2458324.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            7767..9113
                     /locus_tag="KPK67_001332"
     CDS             7767..9113
                     /locus_tag="KPK67_001332"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBG2458325.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            9380..11212
                     /gene="glmS"
                     /locus_tag="KPK67_001333"
     CDS             9380..11212
                     /gene="glmS"
                     /locus_tag="KPK67_001333"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBG2458326.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            11664..12959
                     /locus_tag="KPK67_001334"
     CDS             11664..12959
                     /locus_tag="KPK67_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBG2458327.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            13415..14152
                     /locus_tag="KPK67_001335"
     CDS             13415..14152
                     /locus_tag="KPK67_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBG2458328.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            14173..15426
                     /gene="murA"
                     /locus_tag="KPK67_001336"
     CDS             14173..15426
                     /gene="murA"
                     /locus_tag="KPK67_001336"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBG2458329.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            15513..16577
                     /gene="spoIID"
                     /locus_tag="KPK67_001337"
     CDS             15513..16577
                     /gene="spoIID"
                     /locus_tag="KPK67_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBG2458330.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            16643..17311
                     /locus_tag="KPK67_001338"
     CDS             16643..17311
                     /locus_tag="KPK67_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBG2458331.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            17409..17669
                     /gene="spoIIID"
                     /locus_tag="KPK67_001339"
     CDS             17409..17669
                     /gene="spoIIID"
                     /locus_tag="KPK67_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBG2458332.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            17866..18885
                     /locus_tag="KPK67_001340"
     CDS             17866..18885
                     /locus_tag="KPK67_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG2458333.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            18901..19329
                     /gene="fabZ"
                     /locus_tag="KPK67_001341"
     CDS             18901..19329
                     /gene="fabZ"
                     /locus_tag="KPK67_001341"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBG2458334.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(19440..19949)
                     /gene="yyaC"
                     /locus_tag="KPK67_001342"
     CDS             complement(19440..19949)
                     /gene="yyaC"
                     /locus_tag="KPK67_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBG2458335.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      20138..20241
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            20378..21571
                     /locus_tag="KPK67_001343"
     CDS             20378..21571
                     /locus_tag="KPK67_001343"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBG2458336.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            21887..22984
                     /locus_tag="KPK67_001344"
     CDS             21887..22984
                     /locus_tag="KPK67_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458337.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            23248..25467
                     /locus_tag="KPK67_001345"
     CDS             23248..25467
                     /locus_tag="KPK67_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBG2458338.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFENLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            25470..26270
                     /locus_tag="KPK67_001346"
     CDS             25470..26270
                     /locus_tag="KPK67_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBG2458339.1"
                     /translation="MKICNDNFIKNFNFIKNFKNMINKCLDFIYPENISCIICDKSIK
                     KTNTYSICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISC
                     IEYNDVTKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRG
                     FNQAQKIAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLIN
                     NKNILLIDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            26602..>27427
                     /locus_tag="KPK67_001347"
     CDS             26602..>27427
                     /locus_tag="KPK67_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2458340.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVDKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLI"
BASE COUNT        10996 a         2982 c         5265 g         8184 t
ORIGIN      
        1 tgacatatct atagtagcgt atatttttta caaaatattt atgtttataa aagatacaag
       61 agcagaacaa gtgtttaaag gtataatatt tcttttatta gcaactcaac ttagtaacac
      121 ctttaaattg cataccgttt attggatatc tcttaaagct ttagattatg gagttatagc
      181 tgctcttata atttttcaac cagaatttag agcaggactt gaacatatag gaagagcaaa
      241 gtttaattta tttggaaaga atgtaaatac ttctgaagaa actctcaata gaaatataga
      301 agaaattgta gaagctttat attcactttc tagacaaaaa ataggagctc ttatcataat
      361 ggagagagaa actagaataa gtgatataat aaatacagga actattattg atgctgaaat
      421 ttctaggcaa ctattaataa atatatttat accaaataca ccattacacg atggagctgt
      481 tgtaattaga gattctaagg taaaagcagc tgcatgtttt ttgccattga cagaaagtaa
      541 ggatttaagt aaagatttgg gcactagaca tagagcagga ataggggtaa gtgaggtgtc
      601 tgactgtata actttgattg tatcagagga aacaggtggc gtatcaattg ctaaagcagg
      661 taagttatat agagatattt ctagagaaag aatgatgaat atattacgta gtaatttaaa
      721 aacaaataca gaaacaagaa gttttttcaa aggtggtata tttaaatgat aaatagacta
      781 aaaaataata ccaaaataaa aataatatca tttttaagtg caattgcatt atggatgtat
      841 gttatggctg tagttgaccc tgaagaaact aaagggtttg aagatatacc tgtatctata
      901 agtaatatga gtgatttaaa ggataaaaat cttgttatat atccagacga aaaattaaca
      961 gcagatgtat atataaaagg tagtttatca aaggtacaaa gtgttaaaaa agaagatata
     1021 catgtatatg gaaccattga agacccaata caaggacaga aggaagtata tttaaaagca
     1081 aatattcctc aaggtgttac tctagaattt aagcatgatg gtttagtagt aaatcttgaa
     1141 aaaaacataa aagaaaaaag agatataaag gtagaagtaa gtggaaattc aaaaagaaat
     1201 ataaataaaa ttgaacttag taaggaattt gtcgatgtat caggtccaag aactctagtt
     1261 aaagaagtca aaagtgtaag agctaatcta gatgttggta atgagacaga tgatttttct
     1321 aaaaaattgc aattaatacc attaaatagt gatggaacta aggttgaagg agtaacatta
     1381 agttcatcct ttgtgactgc aaagatagaa ctattgaagg aaaaaattgt cccaataaga
     1441 gtaagtctaa atgataataa agataatgat gtactgaaaa actataaatt aagtaaggaa
     1501 caagttacaa taaaaggaaa gaaagaagat atagacaaaa taagttatat taagacaaaa
     1561 cctattgact tagctgattt ggctaatggt gaagaaaaag aagttagctt agaaattcca
     1621 gagggtataa ctcttgagga tagctctata acaattaaat taaatgatac aaaacaattg
     1681 agtgcagaat ttttatataa tgcgggagat attgaattaa gaaatatccc aacaaatatg
     1741 gataatccaa atgtaatttc tgatgtagat ataaaagttg ttgtagagtc aacggaggat
     1801 ttgagtaagt taaataaaaa agatatttca ttatatattg atttatcaca aggccctgag
     1861 gaagatagta aatataaaat aaaatatgaa acaacatatc aatttaaaaa aataaatata
     1921 gaacctaata ttgttgaagt ggaatgaaca aatatttcga atatttagtt gttacaagat
     1981 gcgacaaaaa aacctataaa attaagtttg ttaattttat gtttttgttg catcttgttt
     2041 attttagtta tattataaaa taaggacaat gagatatttg ttaaatctaa aaagcgctag
     2101 atatatgaaa aaagactgat aaatagattt aaataaatat tttgaattaa atatttcaat
     2161 tatctctagg aaaacgttgc tgaacgagtt ataatttaat tgagatataa aaagttctat
     2221 ttcaaaaaat tcaagatgag gtggaaatgc atgagaagtt ttgaagaagt aattaagttt
     2281 gcaaaagaaa gaggacctaa aactatatca gtagcatgtt gccaagataa agaagtttta
     2341 atggcagttg aaatggctag aaaagaaaaa atagcaaatg ccattttagt aggagatata
     2401 gaaaagacta aagaaattgc aaaaagcata gacatggata tcgaaaatta tgaactgata
     2461 gatataaaag atttagcaga agcatctcta aaatctgttg aattagtttc acaaggaaaa
     2521 gccgacatgg taatgaaagg cttagtagac acatcaataa tactaaaagc agttttaaat
     2581 aaagaagtag gtcttagaac tggaaatgta ttaagtcacg tagcagtatt tgatgtagag
     2641 ggatatgata gattattttt cgtaactgac gcagctatga acttagctcc tgatacaaat
     2701 actaaaaagc aaatcataga aaatgcttgc acagtagcac attcattaga tataagtgaa
     2761 ccaaaagttg ctgcaatatg cgcaaaagaa aaagtaaatc caaaaatgaa agatacagtt
     2821 gaagctaaag aactagaaga aatgtatgaa agaggagaaa tcaaaggttg tatggttggt
     2881 ggaccttttg caattgataa tgcagtatct ttagaagcag ctaaacataa aggtataaat
     2941 catcctgtag caggaagagc tgatatatta ttagccccag atattgaagg tggtaacata
     3001 ttatataaag ctttggtatt cttctcaaaa tcaaaaaatg caggagttat agttggggct
     3061 aaagcaccaa taatattaac ttctagagca gacagtgaag aaactaaact aaactcaata
     3121 gctttaggtg ttttaatggc agcaaaggca taattttaag ggggaaatag gcaaatgagc
     3181 aaaatattta aaatcttaac aataaatcct ggttcgacat caactaaaat agctgtattt
     3241 gataatgagg atttagtatt tgaaaaaact ttaagacatt cttcagaaga aataggaaaa
     3301 tatgagaagg tgtctgacca atttgaattt cgtaaacaag taatagaaga agctctaaaa
     3361 gaaggtggag taaaaacatc tgaattagat gctgtagtag gtagaggagg acttcttaaa
     3421 cctataaaag gtggtactta ttcagtaagt gctgctatga ttgaagattt aaaagtggga
     3481 gttttaggag aacacgcttc aaacctaggt ggaataatag caaaacaaat aggtgaagaa
     3541 gtaaatgttc cttcatacat agtagaccct gttgttgtag atgaattaga agatgttgct
     3601 agaatttctg gtatgcctga aataagtaga gcaagtgtag tacatgcttt aaatcaaaag
     3661 gcaatagcaa gaagatatgc tagagaaata aacaagaaat atgaagatat aaatcttata
     3721 gttgcacaca tgggtggagg agtttctgtt ggagctcata aaaatggtaa aatagtagat
     3781 gttgcaaacg cattagatgg agaaggacct ttctctccag aaagaagtgg tggactacca
     3841 gtaggtgcat tagtaaaaat gtgctttagt ggaaaatata ctcaagatga aattaaaaag
     3901 aaaataaaag gtaatggcgg attagttgca tacttaaaca ctaatgatgc tagagaagtt
     3961 gaagaaagaa ttgaagctgg tgatgaaaaa gctaaattag tatatgaagc tatggcatat
     4021 caaatctcta aagaaatagg agctagtgct gcagttctta agggagatgt aaaagcaata
     4081 ttattaactg gtggaatcgc atattcaaaa atgtttacag aaatgattgc agatagagtt
     4141 aaatttatag cagatgtaag agtttatcca ggtgaagatg aaatgattgc attagctcaa
     4201 ggtggactta gagttttaac tggtgaagaa gaggctcaag tttatgataa ctaatgataa
     4261 aagaataaga atcataactg gtcactatgg aagtggtaaa agtgaatttg cgatgaacta
     4321 tgttgttaag ttaagagata tggtttctgg caaagttgct atagccgatt tagatgtggt
     4381 aaatgtatac tttagaacta gagaaaagaa agagttaatg aaatccttag gtatacaacc
     4441 aatagatagc tcaattaatg ctccaacatt agatttacca gcagtatcag cagaggtaat
     4501 gtctcctatg gtagaccatt catacaattc tgttatagat ttaggtggag ataatgtagg
     4561 tgctagggtt ataggaagat ttagtcattt gttaaaagaa ggcgattatg atatgctttt
     4621 tgtaataaat gcaaataggg agaaaactca aacttctgaa gaagttatcc aatatataaa
     4681 agaaatagaa aaatcttcta agttaaaggt aacaggtcta attaataata ctcatcttat
     4741 taggtttact actattgatg atgttttaag aggtcagaaa gttgcaaaag aagtttctga
     4801 aaaatgtaat ataccgataa gatatgttgt atgtttagaa aatcttgttg aacagttacc
     4861 aaaagattta gaaggagaaa tttttcctat aaaattatat atgagagaag actggatgta
     4921 gtttgagctc aaatatatag aaagtttaag gaggcttagg taaatggcta aaggaaaagt
     4981 atcttttaat caagaacgtt gtaaaggttg tggattgtgt gttgaagcat gtccagtaaa
     5041 aatcatacaa ttagactcaa atgtaataaa caaaaaaggg tataacccag caactgtttt
     5101 tgaaatggaa aaatgcatag gatgtgcaag ttgtgcaact atgtgtccag atgtagttat
     5161 aacagtagaa agagattaat ttaattaata tttaaggggg aattttaatg gctaagatac
     5221 ttatgaaagg taacgaagca tttggaaaag cagctataga agctggttgc aaatatttct
     5281 tcggttaccc aataactcca caaagtgaat taccagaata tttatcaaga gagttaccta
     5341 aaataggtgg agcgttcgtt caagctgaat cagaagtttc tgcgataaac atggtttatg
     5401 gtggagcagg tgcaggagca agagttatga cttcttcatc ttcaccagga gttgctttaa
     5461 agcaagaagg tataacatat gcagtaggag cagaagttcc ttgtgttgta cttaatgtaa
     5521 tgagaggtgg tccaggactt ggaagtatac aaccttcaca agctgactac tttatgtcta
     5581 caagaggtgg aggaaatgga gactacagaa ctccagtatt cgcaccagct acagttcaag
     5641 aagctgttga tatgataatg gaagcttttg acgttgcaga ttattacaga tcaccagtta
     5701 tggtagttgc agatggtatg ataggtcaaa tgatggagcc agttgaattt agagctcctg
     5761 agaagaaaag agaattgcct cctaaagatt gggcaactgt tggaactaaa ggaaaaagaa
     5821 aacctaatgt aataaactct ctatacttag agccagaagt actagaagac cattgttggc
     5881 atttacaaga aaaatttgat gcaatggaaa agaatgaagt acaatacgaa atgtacaaaa
     5941 cagaagatgc tgaatttgta tttgcagctt atggaactac ttcaagagta gtaaaaagcg
     6001 caattgatat attaagagaa gaaggaataa aagcaggtct tataagacct aaagtattat
     6061 ggccattccc attcgaagca ttcaaccaaa ttcctaatgc tagaaacata ttgactgttg
     6121 aaatgagtat gggacaaatg gtggaagatg ttaaaatggc agtagaaggt aaattaccag
     6181 tttacttcca tggaagacca ggaggaatga ctccaacacc agcagaaata gttgaaaaag
     6241 ctaaaaaaat tattgccgga gaattggttg caggaggtgc tagataatgg ctgttgtatt
     6301 taagaaaact gaaggattac aagatactca aactcactac tgtccaggat gtacacatgg
     6361 tataatccat agattagttg gtgaagtatt agaagaacta ggagtattag gtgatgctgt
     6421 aggtgttgtt ccagttggat gttcagtttt aggatataaa tattttaact gtgacactca
     6481 agaagctgct catggtagag cgccagctgc tgctacagga ataaaaagag ttcatcctga
     6541 aaatacagta ttcacttatc aaggtgatgg agacttagct tcaattggta ctgctgaaat
     6601 agttcatgca gctgctagag gagaaaaaat aactactata tttgtaaaca atacaactta
     6661 tggtatgact ggtggacaaa tggctccaac tacattagtt ggacaaagag ctactactgc
     6721 tcaatcagga agaaatgctg aaactcaagg ttatccaata agagtaagtg aaatgttagc
     6781 tacattaaca ggagctgtat ttgtagaaag agtagcagtt gatactccag ctcatgttag
     6841 acaagctaaa aaagctataa agaaagcatt ccaagttcaa caagcaggat taggatttgg
     6901 aatagtagaa gtattatcta cttgtcctac aaactgggga ctagctccaa acgatgcttt
     6961 acaatggtta agagacaaca tgataccata ctatccatta ggaaatttca aaaatgttga
     7021 agttgaggag gtgaagtaaa tgtctacagc tagagtaata tgtgcaggct tcggaggcca
     7081 aggtgttatg tctatgggac aattacttac ttatgcagga atgctagaag gaaaagaagt
     7141 ttcttggtta ccatcttatg gaccagaaat gcgtggaggt acagcaaact gtgcagtaac
     7201 tgtatctaat gagccagttg gttctccact tataacagat gatgcaactg cagctatatt
     7261 attaaatata ccagcatttg aaaaattcaa agatgatgtt attcctggtg gaaaaataat
     7321 agtaaatagt tctttaataa aagaaaaagt tgatagaaca gatgtagatg tttattatat
     7381 accagcaaat gaattagcag ctgaattagg aaatgataaa gttgctaaca tgataatgtt
     7441 aggtgcttac ttaaaagtat ctgatactgt agatatagaa tctgtactag aagcattcaa
     7501 aaaagttttc ggacctagaa aagaaaaatt cgttccatta aacagagaag ctttacaaaa
     7561 aggtatggat gctacatgtt ctaaagcaac taactaataa agaaaaagat gtcaaaaaaa
     7621 agacaataga attatttcta ttgtcttttt tgctgataag tattaaaata tttttatgac
     7681 tgaaattaag cctataatga tattttatga actataaaaa tataatacaa attacaagct
     7741 tttcaattat gaaaggagaa tctttagtga gaaagtattt tggaacagat ggagttagag
     7801 gagtagcaaa tacagaacta acatgtgatt tagcatataa acttggtaga gctggtggat
     7861 ttgtcttagc tcaaggagac cacagagtaa aagtagtagt aggtaaggat actagaatat
     7921 caggtgatat gttggaagca tctttaatag caggacttat gtctgttgga tgtgatgtta
     7981 ttactgttgg aataatacca acacctgcag tggcatattt aactagaaaa tatggtgctg
     8041 attgtggtgt ggttatatct gcatctcaca atcctgttga atataatgga atcaaattct
     8101 ttaataagaa tggatataaa ttagatgatg aaattgaatt gaaaatagaa gaatatatag
     8161 acgatataga caaaattgat tgtcttccaa ttggtgaaaa tgtaggaaga aaattacatg
     8221 aacattgtgc acaaagagac tatgtagact acctaaaatc aataataagc actgacttta
     8281 agggattaaa agtagttttg gactgtgcta atggtgcgtc atataaagtg gcaccaatag
     8341 tgtttgatga actaggagca agtgtaattt caataaatag ttcaccagat ggaaataata
     8401 taaactataa atgtggctct acacatccag aacaacttca aagggctgtt ttggaacata
     8461 atgctgactt aggtcttgca tatgatggag atgctgatag attaattgca gtaaatgaaa
     8521 agggtcaaat tgttgatgga gaccatataa tgatattgag tgccttaaat ttaaagaaaa
     8581 ataataaatt ggcacaagat actttggtag ttacagttat gagtaatata gggttgacaa
     8641 tagctgctaa ggaaaatgga ataaatttat ctactacagc agttggagac agatatgttt
     8701 tagaagatat ggttaaaaat ggatataatc ttggtggaga gcaatctgga catatgatat
     8761 ttttagatta caacactact ggagatggag tgcttagttc tctaatactt gcaaatataa
     8821 tcttgcaaga aaagaaacct ttatcagaaa ttgcatcaat aatgagccaa tacccacaag
     8881 tattagtaaa tgcaacaata aaaaatgaaa ataagaacaa atatatggaa tatccagaaa
     8941 taaaaactga gatagaaaga atagaatcta ttttagacgg aaatggtaga gttttgataa
     9001 gaccatcagg aactgagcca ttagttagag ttatgttaga gggtaaagag gaaggtcaaa
     9061 taaaagaatt agcaacaaac ttagctaatc ttatacaaga aaaattatcg taatttaaag
     9121 ggtattctca gatataaata ctgaggacac ctttctaagc gccagaactt agttatagac
     9181 ataattattg agtaatatag gaactaagtt gacgaggtca gagttaatcg aattgtcggc
     9241 ggatgctctg cggtgtgttt accatcgaaa gacttcttta aataatagga gcaatcctat
     9301 ggacaaaggg aagccaaagt aaacttatat gacctaactc ttaaaatata taaataatat
     9361 ttttaggagg acacaaataa tgtgtggaat agttggatat ttaggaagta gaaaggcagc
     9421 agaagttata gtagaagggc tttctaagct agagtacaga ggttatgact cagcaggagt
     9481 agcagttaat agttctaatg aaaaagaact taatattaga aagtttaaag gtagactttc
     9541 tgtacttgca gaagacttag aaaagaatcc tattgatgga aatttaggta tagggcatac
     9601 gagatgggca actcatggag aaccatctga tgtaaactca catcctcatt ttaatcaagc
     9661 caaaacaata gcagttgttc ataatgggat aatagaaaac tatatggaaa taaaagaaga
     9721 attaatttct gaaggtgtta agtttgaatc tcaaacagat acagaggtaa tagcacattt
     9781 agtagataaa tactatgagg gtaatttatt agatgctgta tacaaaacta tatcaaaatt
     9841 aagaggagct tatgcattag gtgttatatg taaagaacat ggaaatgaat tagttgcagt
     9901 aagaaaagat agtcctcttg tagttggtgt tggtgaaggt gaaaacttta tagcatcaga
     9961 tatacctgca ttattaaaat atactagaga tgtttatttc ttagaaaatg gagaagtagt
    10021 tcatctaaaa gacgaaaatg taacagttta tgactctaat agaaatcttg ttgaaaaaga
    10081 agtatttcat gtaacttggg atgtagaagc tgcgtctaaa ggtggatatg actactttat
    10141 gtcaaaagaa attcatgagc aaccaacagg agttagagaa actttagaaa gaagacttga
    10201 tgataatgga aatataattc tagacagtat aaatatttct aaagaagact tagaaaaaat
    10261 aaataaagtt tatatagtgg cttgtggaac agcatataat gctggattac ttggtaagta
    10321 tgccatagaa aaatttgtaa atataccagt aataactgat atagcatcag aatttagata
    10381 tagcgaccca tttgtagatg aaaattcatt agtaatatta gttagtcaat ctggtgagac
    10441 agcagatact ctagcagtat taagagattc aaaggctaaa ggagcaagaa tactttcaat
    10501 aactaacgtt gtaggttcat ctatagcaag agaatcagat gatgtattct atacttgggc
    10561 aggacctgaa gtagcagttg catcaactaa ggcatatact actcaaataa cttctttata
    10621 catgatagct ttagactttg ctatcaaaaa aggaacaata actagagaat tttatgatag
    10681 tatgatttct aagatgaaag aaataccatc aaaaatacaa gaaatactag ataatgaaga
    10741 atatataaaa gaagttgcta aaacagttgt tagttctgaa catgcattct atttaggaag
    10801 aggtatagac tatagcttag caatggaagg ttcattgaaa ttaaaagaga tttcatatat
    10861 acatgcagaa gcttttgcag caggagagct aaagcatgga acaatagctt taatagaaaa
    10921 aggaactcct gtaattgcca tagctactca agaaaagtta tttgaaaaaa tggtttctaa
    10981 tatggaagaa gtaagagcaa gaggagctta tgtagtagca atagctcaaa gccataataa
    11041 ggatgttgag aaagctgctg ataaaataat ctatatacca aattcagatg atattttatc
    11101 accaatatta gcagttgtac caatgcaatt acttgcttat catgtctctg tactaagagg
    11161 atgcgatgta gataagccaa gaaatctagc taaatcagta acagttgaat aaaaacagat
    11221 taaaaattaa atagataata tttattgaaa agaactcatc ctgttatttg tattttatag
    11281 gatgagtttt ttagataaat attaaatttg ttttgttttt gctataataa attttataaa
    11341 aagattgata ttaaatcatc tgtaaaatga taaatatata gaccatacca ggtataggga
    11401 acgataaata tagttcatat aaaatatagt gagtacttaa taaacatata gtcaatatca
    11461 gataaaaaaa ataatagctt atgagaaaaa ttaaaataca aggtattagt aaccttgtga
    11521 taagataaag tgagtagttt aaaataagaa tataaggaac tatttataaa agaatataat
    11581 gaagctaata gagtataaga aaattacttt attttagtaa aataatttca aataaaaatt
    11641 tttttaaatt gggagtataa attatgacaa atgattttaa aaaagaaaag aaagaagatt
    11701 attttgttaa tcttaaggca atatctactg aagttttaca agaaaaagtg caagataacg
    11761 cttgggttat agttgatact cgcttaaatg atgcttataa tggctggaag ttagatggag
    11821 taaaaagagg tggacatata aaaggagcag tagatttttc tgctaattgg ttaagtgtat
    11881 actcagatag aaaagatgaa gtgttagaac aagcattgaa aactaaaaga attgatttag
    11941 ataaaaatat agtattgtat gatgcaaatg gaaaagatgc tttggttgta gcagattatt
    12001 tatctaaaaa aggatataaa tatctatata aatatgatat taaacaatgg gcagacgacg
    12061 aaaatcttcc aatggaaaga tataaaaatt atcaaatgat agttcctgct tttataataa
    12121 aagatatatt agatggaaaa attcctgaga catttgaaga ttcaaagaat ataaaaatga
    12181 ttgaagcgag ttggggagaa gaatcgtata caaaagggca tatacctaca agtgttcatg
    12241 taaatactga tattatagaa cctcctccaa catggatgct tgataatgat gataacttaa
    12301 ctaagtttgc tttagactat ggattaacaa aggatgatac tgtaatagtt tcaagtagta
    12361 cacctatggc atcttataga ctagctgtta tattgaggta tataggagta aaagatgtaa
    12421 gagttttgaa tggtggaaca aactcatggt tatcagcagg atatgaatta gaatttatta
    12481 gtaatccaaa acattcatgt actaattttg gagcagatat accagttaac tcacaattaa
    12541 ttgttactac atctgaatta agacaaaaac taaaagaaaa aaataagttt atactagtag
    12601 ataaccgtac ttgggatgaa catataggaa aagtatctgg atatacatat tatgataaaa
    12661 aagggcgtat accaggagct ttatatggac attcaggtag tgatagtgtt tcactagaag
    12721 aatatcgtaa tattgataat acaatgagaa ataaatatga gattttagaa atgtgggaca
    12781 aggaaaatat agatgtgaat aaacagctta tatttatgtg tggaagtgga tggcgtgctg
    12841 ctgaagtatt aacatatgca aatgtcatag gagtggaaaa tacctccctt tatagtgatg
    12901 gatggatggg ctggagtctt gataattcaa atttaattga agtgggagaa cacaaataaa
    12961 gtttttgaaa gtgcaataga gttgtttaaa acaaaggtgt ttttgattta ttaggaagag
    13021 tgtgtaaatt gtgtattggg aagtgtactt aggtatgttc atttggagct atggaaactg
    13081 ataaaaaagt attatctgtt tattttgttg gtaaaatatg aatagattat agattataga
    13141 ttatagatta tagattatag atttaagtat agactaagta atttatatta cagaagagaa
    13201 tatataagaa ttaatgcaag atgtatttga tacatataag aaattggcaa gtccaggaga
    13261 aggatagaga aaattgagat tgaagagttt gaaagagatt attagatgaa aaatacaaaa
    13321 atgtataaaa atacatattg agaaagtata tctctcttaa atttgttaat aattctatta
    13381 tacagaaaaa catatttaag ggggattttt tactatgaaa aaagtaaaat tagctataag
    13441 cttttttata ttaatgatta taggaatgac aatatcttat gcagatacaa gtataaatac
    13501 tagatacaat agattaagta aagctcttga aatatcagaa gcaaatttta aattttataa
    13561 tgttaaaatt aattctgtta taaatcataa tttatcaaat aaagaatttc ataatatttt
    13621 tatagaaata attaacaatc taaagttgga taaaaaagga ataaattgga atcaaaaagg
    13681 gtctggcgat aaaatacaat tatgtgctac tataaaaggt agcgataaag aaatttctat
    13741 aaaaggaata aataaaaata acaaagaaag ttacatagta gttgacatat tggataacaa
    13801 agtatataaa cataaagagg ttatttatag taacgtagaa aatgtgttaa ataaatattc
    13861 atctcaagta gatgtgtctg cttgtattgt tggagaatac acaaaaaggt tacaaaacaa
    13921 caaatatgat gatattttac aaaaaatatt atataatatg agtgctaagc aaatagatga
    13981 agttaaagac gtaaactttt tatctataac agcatatagt aaagtattaa atgattatga
    14041 attagaatat ttaggggaaa aaattaattt aaatatagga attaaatata atgaggatga
    14101 cggaaaaaca ctcatatata ttgccacacc aattataaaa ttagattatt aattatgagg
    14161 agatcaaaaa atatggctaa gataatagta aaaaagagta atccacttaa aggtagtgtt
    14221 aaaatagatg gtgcaaaaaa tgcagtttta ccaataatag cagcgacttt attagcaaac
    14281 ggaaaatcaa cattaaatgg agtaccaaat ttaagagatg ttcatgttat atcagattta
    14341 ttaagacatg taggagcaga agttgaatat aaagaaaata cacttactgt tgatgcaagc
    14401 aatataaaaa cttgtgaagc tccatatgag cttgtaagaa agatgagagc ttcttttttg
    14461 gttatgggac cattgcttgc tagatttaat agtacgaaaa tttctatgcc aggaggatgt
    14521 gctataggaa caagacctat agatttacac ttaaaaggat ttaaagcttt aggtgcaaag
    14581 atagaaatgg accatggttt tgttgaagca gcaacagaaa agttagtagg aaataaatta
    14641 tatttagatt tcccatctgt tggagcaact gaaaacataa tgatggcagc ttctttagca
    14701 gaagggacta caataataga gaatgcagca gaagagccag aaatagtaga tttagcaaac
    14761 ttcttgaacg aaatgggagc agatgttaaa ggtgcaggta ctaatactat aaaaataaaa
    14821 ggtgttaaag agttaaaagg agcagaacat aatgtaattc cagacagaat agaagcagca
    14881 acttatatgg ttgcagcagc tatgacaaaa ggtgatataa ctgttgaaaa tgttttaatg
    14941 gagcatttaa aaccagttgt agctaaatta agagaagctg gttgtgaaat aacagaaatg
    15001 gacaattctg taagagttgt aggtccaaaa gtattaaaac caatagatat aaaaacttta
    15061 ccacatccag gatttcctac agatgttcaa gcacaattta tggctatgct tactgtagcc
    15121 aatggaactg gtgtagttat agaaactgtt tttgaaaaca gatttatgca tgttgctgaa
    15181 tttaatagaa tgggtgctaa tataaaaata gatggaagaa gtgcggttgt taatggtgta
    15241 gatgagctac acggtgcagc agtaaacgct actgatttaa gagcaggggc agcccttatt
    15301 ttatgcggtc ttatagcaga aggagaaact caaataggag aaatatacca tatccaaaga
    15361 ggttatgttg atatagataa gaagataaca gcactaggtg gtcaaatcga aatagtagaa
    15421 gactaataat tggtttatag aagaaagtta taaattaata gggttgttca tatacttaaa
    15481 tacaaggttt taaaagctaa ggaggagagt atatgaagaa cccattggtt gttttgttag
    15541 gatttgtgac ttgctctgtg ttagttccat cacttataac tttagtttct tataaaaatg
    15601 tagaattaac tgaaaaacca gagagcccag tatctataaa caaaactatt aaaaaaagtg
    15661 atattgaaga caaaggtaat aaagaagaaa aaaatacagt gaactatgaa actgtaaata
    15721 aaaaagcacc aattatcaat gtatataatc atataacagg gaagaccgaa aagatggaca
    15781 tggagaacta tttatgtgga gtactagctg gtgagatgtc ttcagagttt gatatagaag
    15841 ctttaaaagc tcaatctgta gcagctagaa catatgtagt atataaacag gaacatggta
    15901 aatctagtaa acataaaaat gcagtagtat gtactgatta taaacattgt caagaatata
    15961 agagttatga tactcttaaa aaattaaatg gtgaagaatg gattaagaat aaatattcca
    16021 aaatccaaga agcagttaga ggaactaaag gtcaaataat aacttataat gataaggcga
    16081 tacttcctct ttatttttct acatcttcag gaaaaacaga aaatagtgaa gaagtatttt
    16141 ctgcaaaata tccttattta aagtctgtgg aaagtcctta tgataagtat tcccctaaat
    16201 ttgcgtcaac acttaaaata agtaacacag attttgttaa aagtcttaga agggcatata
    16261 gcactattgt gatagatgtg aataatttaa gtaaacaagt ttcaataact aaaagaagtg
    16321 atgccggaac tgtagaaaaa ataaaattag gaaataaaga attgactgga aaagacatta
    16381 gaactgtttt taaattgaac tctgcaaact ttgacataaa atttggagaa ggatacatag
    16441 atttcgtggt aaaaggatat ggtcatggtg ttggtatgag ccagtgggga gcagaaggaa
    16501 tggcagaaga gggctataaa tattatgata tattatcaca ttactacaca gatacaaaaa
    16561 taaaagatat atactaataa ttgtataaaa tgccaaaaac acgtcaataa tccaaatgta
    16621 gaaatatatt tggaggtgca aaatgaagaa aaagctgtta gaaaaagatg gtttttattt
    16681 atctttgttt gtatgtgttt gtttattagc agtaggtgga gtttggttta caaataataa
    16741 tgtagataaa ttggcttcta ataaaggaat tatggaaaat gctaataaag atagtgaaga
    16801 ggaaatacat ttaattgaaa aagacaagaa agatgctatc cctactgcaa cggattcaaa
    16861 acaaaactta gaaaaagcta agtcaaaaga ggaaaataaa tcgagtacaa caaaattaaa
    16921 ttacattgga gataaagtaa taagaggata ttcagaaaaa gagcctagtt attctaaaac
    16981 acttgatgtt tgggaaactc ataaaggtgt agatattagt tgtactaaag gcaaagaagt
    17041 aaaatctcta ttaaacggaa tagtagtaga tgtatttgat gatgaagaat atggacaatc
    17101 agtcaaaata aaaagtgaca ataacattgt agtagtatat tcaaatctag ataaaaatgt
    17161 tagtgttaag aaagaacaga aggtaacaga aggacaatct ctaggtactg taggtagtac
    17221 ttctcaaata gaaagtgaag aaggtataca tgtgcattta gaggcgtata gtggagaaaa
    17281 atctatagac ccaatgagtc taattaagta atagtataac aagtttaatc atatttggtt
    17341 aagcttgtta tattttttgg tgtttttaca tatatatcat taaagctatt atgtgagggg
    17401 ggtaatcttt gagatctcat atagaggaaa gggccatagt ggtagcaaaa tatatacttg
    17461 aaaaaaacac tacagtaaga caaaccgcca agacatttgg agtaagtaaa agtacaattc
    17521 ataaggatgt tacagaaaga ttgaaggaaa taaatccatc ccttgccaaa gaagttaaaa
    17581 atgtgctgga caaaaataaa tcagagagac atattagagg gggattagca acgaaattaa
    17641 aatatgagaa ggaacataaa aaaatgtagg ttaaaaacct acttttttta ttgaattaaa
    17701 ttataaaaag aaatatatta agtaattata aatagcaaaa taataatact ctcaaaagat
    17761 ttattttctc ttgattaaag cgagattttt gtatatgctt tattatagga gtattaaaat
    17821 aggcttataa tataatctaa tattataaat aaaggagttg aaaaaatggc tggagctgat
    17881 ataggcatag atttaggtac tgcaaatgtg cttgtttatg ttagtggtaa gggaatcgtt
    17941 ttagaagaac catctgttgt ggcgatagac aaaagaacag attctgtttt agctgtggga
    18001 gaagaagcta aaaagatgat agggagaaca ccaggtaata tagtcgctat aagaccttta
    18061 agagatggtg taatttcaga ttatgatgta actgagaaga tgttaaaatc ttttatagat
    18121 aagatagttg ataaaaaggg atttggaaga ttttttatgc caagaattat ggtatgtgta
    18181 ccaacaggtg tcacagaagt ggaaaaaaga gcagtagaag atgctacgag acaagctgga
    18241 gctagagaag tatatatcat agaagaacca atagcagctg ccataggagc tggtgtagac
    18301 atatcacagc ctaatggtaa tatgattata gatattggtg gaggtactgt agatatagct
    18361 gtaatatctc taggaggagc tgttgtaagt gaatctataa aggttggtgg agatagattt
    18421 gatgatgcta tagttaaata tatgaaaaaa caacacaatc ttcttatagg agaaagaaca
    18481 gcagagaaaa tcaagtttga aataggttct gcatttaaga gagaagaaga aaaatatatg
    18541 aagataacag gaagaaattt aattacagga cttcctagtt ctataactat aaattcaaca
    18601 gaaatgttgg aagcacttag agaatgtgtt gagcaaatag tagttgctac tcatgctgtt
    18661 ttggaaaaaa cgcctcctga gttagcagca gatataggag atgtcggtat aataatgact
    18721 ggaggaggtt cattattata tggattagat aaaataatag aacagaggac aggaataact
    18781 gttacaatag cagatgaacc attatcttgt gttgcaaaag gtacaggaat agctttaggt
    18841 tcaatagact tacttgaaac tggtgggtct ttcaaaagaa aataattagg agagtttaga
    18901 atgttaaata tagatgaaat aaaaaaatta ataccacata gatatccatt tttattagta
    18961 gacaagatta cagagctaga agttgggaaa agagctgttg gtataaagaa tgtaacagtt
    19021 aatgaaccat ttttccaagg tcactttcca gaatatcctt tgatgccagg agtactaata
    19081 gtagaagctc tagcacaagt atgtggagtg gcaatgatgt ctgtagagga aaacaaagga
    19141 aaactaggtg tatttgcagg tatagataaa gttagaatca agagagaagt aagaccagga
    19201 gatacattaa ctatggaagt tgaaatgaca actcttagaa aaaatatagc taaggcagat
    19261 gctaaggcat atgtaggtga agaacttgtc tgtaaaggtg aattaatgtt tgctttagta
    19321 gaaaaataaa aacttgaaat ataaaagtaa aaaggtagat aatttggagt atttaatcag
    19381 atactctaaa atatctacct ttttatataa agaattttat catttagttt atatttagat
    19441 taggtggata ctaataaagc caaggctatt gtttcagcta aatctaatat aaagctaagg
    19501 cgtatattat taaatgaaag tttattattt tcatcaattt tatctacaat tcctactata
    19561 gaataatttc ctatttgagg tagtttttta ccaacacctt ttccgggcag gataggtcct
    19621 tctctaatct gtatattacc tatgttactc tgcgacccca agcaagcatc tatagccaag
    19681 aagttacctt gtatatgtgt attttttata gtatctaaag actcatatat atttaaggcg
    19741 tgaatagggt tatctagtgt accgtaaacg gggtatttaa agttactgtt cttaagtatt
    19801 gttccaacta aagggcctaa agtatcacct atagccctgt ctgttcctat gcaaacaaca
    19861 atcgtatttt cattaattat agtttttaga actgaactta gcatctgtat aacatcatca
    19921 tcgctgtagt ttactttagc taaatacaat aggaacaccc ccttcaaaaa aatatatgaa
    19981 taaaaagcat taaaaagacc taactttaaa tttcagatag atagaaaatt ttatttgtct
    20041 tacaaaaaaa ggaatagaaa aaatatgact aaaaatattg acaaaatttt aaaaaaatag
    20101 taagctagta aagtaaaaca aaacttaaat cttgattacc ttatcaagag aggctgaggg
    20161 ataggccctg tgaagcccag caaccaccca aatgggaaaa ggtgctaatt cctacaagac
    20221 aaaagtcttg aaagatgagg tcagttaatt agtatgtaat tacgattatt aacctcttct
    20281 tataaaggag aggttttttt attttgagta aatttctaaa attaaaaata aggtaaaacc
    20341 tataataaaa attaagaata aataaggggg aataaaaatg gcaagacatt tatttacgtc
    20401 agaatcagtt acagaaggac atcctgataa gatatgtgac caaatatcag attcaatatt
    20461 agatgcatta ttagaaaaag acccacaatc tagagtagct tgtgaaacta cagttactac
    20521 tggtttagtt ttagtagcag gagaaataag tacatctgct tatgtagaca ttcctaaatt
    20581 agtaagagaa acagtaagag aaattggata tacaagagca aaatatggat ttgattgtga
    20641 tacttgcgca gttataactt ctattgatga acaatctgga gatatagcta tgggagttga
    20701 tgaaggtcta gaaagtaaga ctggagaaga gatagaagaa gaaatagaaa aagttggagc
    20761 tggagaccaa ggtataatgt ttggatttgc atgtaatgag actccagaat taatgccact
    20821 tccgatatct ttggcacata aattatcaag aagacttaca gaagttagaa aaacaggttt
    20881 agttgattat ttaagaccag atggaaaaac tcaagttact gttgaatatg aaggaagtaa
    20941 agctgtaaga gtacatacag ttcttatatc agctcaacat tgtgaaacag tatcaaatga
    21001 taaaataaga gaagatttaa ttaatcatgt tattaaggaa gttataccag cagaattact
    21061 tgatgaagag actaaaattt atataaatcc aaccggaaga ttcgttatag gaggaccaca
    21121 aggagataca ggtcttacag gaagaaaaat aataatagat acttatggtg gatattctag
    21181 acatggtgga ggagctttct caggaaaaga ccctacaaaa gttgatagat ctgctgctta
    21241 cgcagctaga tatgttgcaa aaaatattgt tgcggctggt cttgcagaca aatgtgagat
    21301 agaacttgca tatgctatag gtatagctag accattatct atatttatag atacttttgg
    21361 gacaggcaag gtttcagaag aaaaacttgt tgaattagta aataaacact ttgatttaag
    21421 accgggagcg ataataagag acttagattt aagaaagcct ctttataaaa aggttgctgc
    21481 ttatggtcac tttggtagaa ctgatataga tttaccttgg gaaagaactg acaaggttga
    21541 gcagttaaga aaagatgctt taggtgaata atttttaact aaaaagctgt tagaatgtaa
    21601 ttttacgatt actttctaac agctttttta ttatgagtta aatattttta ataaatgtta
    21661 atttatcatc atataatgaa taaacagtat ttaatctggt caatacattt tatataatac
    21721 tctactaaat ttatacatat tatgggatat atgttaaagg atggttaaga atgtgttaac
    21781 taatgttaaa taattaaaaa tatattgaaa tatcacaata atgagtctaa tataatctta
    21841 ataaacttac actttaaaaa taattttaat tgggggggag tagtgtatgt caggcaaagt
    21901 aaatattaaa acggttataa gttttgctgg agcttatgtt gctactgtaa taggttctgg
    21961 atttgcaact ggtcaagaaa tactgcaatt ttttacgttt tatggatatg cgggtattat
    22021 aggtggaata atctctatgg tactattttc atggtttgga gcagaagtta tagataaggg
    22081 aagagaattg aaacttaagg agccaataaa aatttatcaa gtatattgtg gaaaatatct
    22141 tggaacattt tttgaatggt ttggtccatt gtttttattt ggaacattag taataatgat
    22201 tgcaggagca ggagctacat tatcagagta ctatggatta aatccatatg taggtaggat
    22261 tggtatggca atcgtttccc taataactgt ttcattaggt ttgacaaggc tttctaaaat
    22321 actaggcaat ataggaccta taataataat atttacactt ttagtaggag caattagttt
    22381 atttagcaat attgatgctt tatcaaatgc tggaaatatg gttgatagtt taaatattaa
    22441 aactgctaca tcaaatggat atttctcggg agtactttat acttgttaca atgtaataat
    22501 agtaataaca ttcttgacag gaatgggagc ttctgcagta aataagaaag atgctgtatg
    22561 gggtggtata gtaggtggag tagcacttat ggcagcagct ataatgatga atttggcatt
    22621 actttcagac ataggaaata tatacacaaa agagatacca gcactttact tagctgataa
    22681 gatatctcca ataataggta ttttattctc agtagtactt ttactaggaa tatatactac
    22741 agctgtacca ttgttatggt cagtaacaaa ccgatttgta gaagatgacc accctaagtt
    22801 taaaattata acaatagtgg taagtatatt agcttgtata ggaggtttac tgccatttga
    22861 taagttagta ggtacactat atccatatac aggttacatg ggaattttaa ttttactatg
    22921 tatgctttat agaagaatta ctaaaactga ggggtacaag gagaataaat ctgaaataag
    22981 ttagtactgt aatatactta tgaaatttaa tttatactct aaatatagag taattaaaat
    23041 aaaatagcta tttaaatatg gtacctgtat ttcatggtac catattttat ggatgcagat
    23101 tttttttatt tctgataaaa tgtataatag ttggaaatta cgttaagtat aaaaatataa
    23161 aacttttcta cgattatcat agttttaata tataaaatta tactaacatt taaatggagc
    23221 gattattagt atttttagga gataaaaatg gaaaaattag aaggaatgat aagtgaaata
    23281 gtctttaaaa acgaagataa tggttataca atagctcacc tggtaaatga aaatgatgaa
    23341 attgtggttg taggatgtat gcctactttg gcaattggag aaagcataga agttgaagga
    23401 aagtgggtaa atcataagat ttatgggact caatttgaag tgaatagttt tatgccagta
    23461 acaccatcat ccttagaagg aatttatgtt tacttatctt ctggaatgat acatggtatt
    23521 ggtgaaaaaa tggcaaaaag aataattgat aagtttggtg tagatacgtt agaggtgata
    23581 caaaattcac cagaaaaact acaagaagta gaaggtatag ggagtaaaaa agtaaaacaa
    23641 atagtaaaaa gttatgaaga agacagagaa ttgagaaata taataataca gctatctcct
    23701 tttggaataa caccaaatta ctgcctaaaa atatataaaa aatataaaag cagtgcaata
    23761 gaagttataa ataaaaaccc ataccaactt gctgaagata taagaggaat tggatttaaa
    23821 gtagctgata gtatagcaag taaaattggt atagataaaa actctaaaga tagaatttgt
    23881 cagggaatct tatacacttt aaataaatct ttaagtaatg ggcatacata tttaccagag
    23941 catgttctaa tacaagattc ggaaaaatta ttggagctaa atggagaaat aataaaagag
    24001 tgtgtaatga tgctagttta taatcaaaaa atacatatag agaaagtaaa caatgaaaat
    24061 cttatatacc ttatgccata ctatttagct gaaaatggag tatgtagtca aatagtaaaa
    24121 ttatcacagt atgaatttga aaacttaaaa atagatatag ataacgagat taatgtttta
    24181 gaagaagata agaaaattaa gttagcagaa aaacaaattt tagcagtcaa agaatctgtt
    24241 aatagtggag tattaattat aactggtgga ccaggaactg gtaaaacaac taccattaat
    24301 gctatcatag atatatttga aaataatggt aaaagtgtga ctttggcagc tccaacggga
    24361 agagcagcaa agagaatgag tgaaacttct aataaagaag caaaaactat acataggttg
    24421 ctagaaatgg gattttcaac agatgatgat ttaacttttt ttaaggatga agaagaccca
    24481 ataaattcag atgtaataat tgttgatgag gtatcaatgg tagacattat cttaatgtac
    24541 aatttactta gagctataaa gttaggaaca agagtaattt tagttggaga tagtgaccaa
    24601 ttaccttctg taggtgctgg aaatgtactt aaggatatga ttaattctaa tattataaat
    24661 gtagttaaat taaatgaaat atttagacaa gcgcaagaaa gtatgataat tgtaaatgcc
    24721 cataagataa acaatggaga accactttat ctaaatacaa aaggtaaaga tttctttttt
    24781 ataagaaaat caactaatga agaaatttta aatgaaataa taggattggt taatgaaaga
    24841 ttacctaaat tttataaagt tgataaatta aaagatatac aagttttatc ttcaatgaga
    24901 aaaggtgaat tgggagttac taatctcaat atagagttac agaagtatct taacaaaaaa
    24961 gaaaaattta aagttgaaga aagtttttct aaaagattat ttagagttgg cgataaggta
    25021 atgcaagtaa aaaataatta tacaaaaaaa tgggaaacag aagaccaaaa agaaagtgga
    25081 gaaggtatct ataatggaga tataggatat gtttatcaca tagataaaga taagaaaact
    25141 atttatatat tatttgacca gactaaaatt gtatcatacc tatatgatga attagatgaa
    25201 attgaccata gtttttgtac aacaatacat aaaagccagg gaagtgaatt tccagttgta
    25261 gtattgccaa tagcgtgggc tcctcctatg ttacttagcc gtaatttatt atatacagca
    25321 gtaacaagag caaaaaaatt agttgtatta gttggagatg tgaaatatct tgaatatatg
    25381 ataaaaaata atagagttaa tcaaaggtat tcaaatttag gatataagct aaataaattt
    25441 aaacaagaag ggttgttgat agaataaata tgaaaatatg caatgataat tttattaaga
    25501 attttaattt tattaagaat tttaaaaata tgattaataa atgtttggat tttatatatc
    25561 ctgagaatat aagttgcata atttgtgata aatccataaa gaaaacaaat acttattcaa
    25621 tatgtaaaag ttgttttaaa gaaatgaatt ttatacaaga tggttgcatg aagtgtggaa
    25681 aaccaataat acgtcattca attgaaaaag aatttattga agagtgtagt tattgtttta
    25741 ataaagactt ttattttgat aaatcaatat cttgtattga atacaatgat gtgacaaaaa
    25801 agatggtgtt agggctgaag tataaccaaa aaacctttat ggctaagtat atagctcaaa
    25861 taatgaaaga aaaattatat ttagaaaaca taaaatttga ttatatatta tttgttccac
    25921 ttcataaaaa aaggctaaat aaaagaggtt ttaatcaggc acaaaaaata gcatttaatt
    25981 taagtaaaat agtaaatatt ccattattag attgcataag tagaaaaaaa tatacaagaa
    26041 tgctatataa attgaataag aaagaacgaa aagaagaatt aaaaaatgta tttgttgtta
    26101 aagaaaatgt taaattaata aataataaaa atatactttt aatagatgat atatttacta
    26161 ctggtctaac aacaaacgaa atttctaaac ttttaaagtt atcaggtgct aataaggttt
    26221 ttgtattgac tctacttaca aaagcaaatg ataattatat tatggaataa tcttatatat
    26281 tgacaatttt agtttaaaat attaaaatga ataagtgata tttaggtctg tggttgaaag
    26341 tccaagccag tcgcaggcaa agggatccac gtaagttaac actaaaaatt gggttaatga
    26401 tcatggtgcg gcttagaagt aagacctacc gataataaaa tcgagagaga gtagtagtgc
    26461 ggcgaaccca agcgaaccct ccagtaggcg agtgtggggt caaaaaccag gtcagctaaa
    26521 tatcatatta aaagagagct agcatacctt tatgtatagc tcttttttat atataataaa
    26581 tttaggttga gtcataaaga ggtgatatgt ttgaataaaa gacaagaaaa tttattaaca
    26641 acattgctat taaaagaaga gtttataaat gttgataaga ttgccaatga ttttgagtgt
    26701 tcagaaagga caataagaaa tgattgcaaa tatattgatg aatggttatc tactttttgt
    26761 aatgcttata tcaaaagaaa acctaatatt gggattaaat ttagtggaga tggaacagac
    26821 aaaatactgg taaatcagaa gttgcgaggt gaagttaaaa gagtaaatag tgagcttcat
    26881 aagcaagttg agatattaaa tttactactt tgtaataata aaaaagttac tttaaatgat
    26941 ttatctaata agttgtacat aaataaaaat attgtcagag aagaaataag taaattatca
    27001 gtagcattaa ataattataa tttaaatatc tcgactaaaa aaggcagtgg tatatatatt
    27061 gaaggggatg agaaagacat aagaataatg ttagtatcat tcttatttaa atatatagat
    27121 aaatacagat taaacataga tgaaatagag tacttccata tagtagatat aataattgta
    27181 aaaaacataa tgcttactat agaggaggat atggatataa gacttacaga tatatcattc
    27241 aagcaattga tgctattttt gcttataaat ataatacgaa taaggctggg aaattcttta
    27301 aaagtatcaa atactaagaa agagcttaag ctaaaaatgc ttatattgga tatagaagat
    27361 gaattgaaat ctaatttatc catagtatta aattatgaag aaaaattgtt tatagaatct
    27421 ttaatat
//
LOCUS       DAEMHW010000037        26762 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEMHW010000037 DAEMHW010000000
VERSION     DAEMHW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.037"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            182..1138
                     /locus_tag="KPK67_001348"
     CDS             182..1138
                     /locus_tag="KPK67_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG2458341.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVDIP
                     GHGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            1423..2388
                     /locus_tag="KPK67_001349"
     CDS             1423..2388
                     /locus_tag="KPK67_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG2458342.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(2586..4697)
                     /locus_tag="KPK67_001350"
     CDS             complement(2586..4697)
                     /locus_tag="KPK67_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBG2458343.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(4999..5706)
                     /locus_tag="KPK67_001351"
     CDS             complement(4999..5706)
                     /locus_tag="KPK67_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG2458344.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYAKRLITATRVLQ"
     gene            complement(5905..7818)
                     /locus_tag="KPK67_001352"
     CDS             complement(5905..7818)
                     /locus_tag="KPK67_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG2458345.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDVCKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEEKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            complement(7902..8993)
                     /locus_tag="KPK67_001353"
     CDS             complement(7902..8993)
                     /locus_tag="KPK67_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG2458346.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            complement(8999..10342)
                     /locus_tag="KPK67_001354"
     CDS             complement(8999..10342)
                     /locus_tag="KPK67_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBG2458347.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNDVK
                     VELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEKIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            complement(10320..11072)
                     /locus_tag="KPK67_001355"
     CDS             complement(10320..11072)
                     /locus_tag="KPK67_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2458348.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            complement(11120..12028)
                     /locus_tag="KPK67_001356"
     CDS             complement(11120..12028)
                     /locus_tag="KPK67_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2458349.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            complement(12050..12799)
                     /locus_tag="KPK67_001357"
     CDS             complement(12050..12799)
                     /locus_tag="KPK67_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2458350.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            complement(12828..14003)
                     /locus_tag="KPK67_001358"
     CDS             complement(12828..14003)
                     /locus_tag="KPK67_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBG2458351.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            complement(14018..14857)
                     /locus_tag="KPK67_001359"
     CDS             complement(14018..14857)
                     /locus_tag="KPK67_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2458352.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKNYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            complement(14860..16017)
                     /locus_tag="KPK67_001360"
     CDS             complement(14860..16017)
                     /locus_tag="KPK67_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBG2458353.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            complement(16033..16755)
                     /locus_tag="KPK67_001361"
     CDS             complement(16033..16755)
                     /locus_tag="KPK67_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG2458354.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTYLMEKNYK
                     KIDPNNYEEGIHVYDKEYFYPFGFKEDFTLECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            complement(16767..17840)
                     /locus_tag="KPK67_001362"
     CDS             complement(16767..17840)
                     /locus_tag="KPK67_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBG2458355.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            complement(17862..19568)
                     /locus_tag="KPK67_001363"
     CDS             complement(17862..19568)
                     /locus_tag="KPK67_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG2458356.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEENNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            complement(19614..21170)
                     /gene="murJ"
                     /locus_tag="KPK67_001364"
     CDS             complement(19614..21170)
                     /gene="murJ"
                     /locus_tag="KPK67_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG2458357.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            complement(21285..22340)
                     /locus_tag="KPK67_001365"
     CDS             complement(21285..22340)
                     /locus_tag="KPK67_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBG2458358.1"
                     /translation="MIKKNSTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            complement(22435..23121)
                     /locus_tag="KPK67_001366"
     CDS             complement(22435..23121)
                     /locus_tag="KPK67_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBG2458359.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            complement(23341..25365)
                     /locus_tag="KPK67_001367"
     CDS             complement(23341..25365)
                     /locus_tag="KPK67_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2458360.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            complement(25619..26677)
                     /locus_tag="KPK67_001368"
     CDS             complement(25619..26677)
                     /locus_tag="KPK67_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBG2458361.1"
                     /translation="MIVCNNPGTWMRMYPQLRHAVWHGVTLADFAFPFFVISLGVTIP
                     ISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNPDLDTVRILGVLQRMGLVY
                     FVTSLVYLLLKKLNVGSTATIITFLCISTFIIVGYYILAKPYGFELEGSLAQLVDLHF
                     FKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENLGEYKKFFKILVMSIILLI
                     CAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICDIKNKSKIFTPIIALGSSP
                     IFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELITPWAGTTWDSLIFSLLYVL
                     FWVIVMSIMYKKKIFIKI"
BASE COUNT         8460 a         4782 c         2948 g        10572 t
ORIGIN      
        1 catacaatta tattccaata aaatcaataa taactacaca ctttcttcta taaaatgcta
       61 taaaaataat tatattatga caaacttatg ctaatttatt gtgtttttta ttgcttatgt
      121 aacaaatttt taatataatt aactatgtgt aattaaaaaa tttagaaagt aggggatatt
      181 attgtcagga ctcaaaaagt tcgttatatt tctagccctt ttagttatag catttcctgt
      241 tagtatatat ggatatgttt ttactaaatt aaaaactata caagatgata gtataaatac
      301 agaggcgctt agtagtaatg actataaaaa cgaagaaaat atcacaaaca tattattgtt
      361 aggtacagat gcaagaccag gagaagaagt ttctcgttct gatgccatga tgatacttac
      421 tattgataat aaaaataaaa gtcttaagct aacttcatta aatagagata cttatgttga
      481 tataccagga catggagaac aaaaattaac acatgcctac gtatatggac aagctaactt
      541 attaatagaa actatagaag aaaacttcca acttgatata caaaactacg ccgtagttga
      601 ctttttctct tttatggata tagttgatgc tttaggtgga gttactgtta ctgttgataa
      661 gagtgaaata agagaattaa ataaatttat tcctgaaact tataaatgga ataaaaatga
      721 caataaaggt tctcttaaat atataagaaa ttctggtgag caaaaattaa atggatatca
      781 gacacttgca ttttctcgta taagaaaaaa tgatagtaca gatgaaagag atagaagaca
      841 aagaagtgtt atacagagtt taataaatgg tgttaaggat ttaccagtca ctaaatatcc
      901 taatctagtt aacacaatac ttccgtatgt aaaaactaat atgaatccta atgaaataat
      961 atctttagga aaagaacttt taagtatagg aaatttaaat ttgaaatcta tggagtttcc
     1021 tctttctact gaaaatggta gaaaaatagg taatgcaggc tatgtaatac cttttgagga
     1081 atatgaacta gatgcaatgc atgatttcat atttaaagat attatggttg aagattagaa
     1141 attccaaaac aaaccaataa tttgatgata aatacatacc tcttaaatgc aaattgcttt
     1201 gtatttatgg ggtgtgtttt tattttaaaa tataaattgc ttttaaaaaa tatattatat
     1261 atacttttaa ctatatatat aggtcgataa attttcttat aaattatatc attgtctttt
     1321 ttttgtaact gaaacaagta ttgaatatat ctcaatactt taatataatt aagtgtatgc
     1381 tattaaaaaa tatacaggaa aaagaagaaa gaaggggtac ttttgtcaaa attaaagaaa
     1441 tttgttatac tactggcttt cttggtagta atattcccta taagtgtcta tggatatttt
     1501 tattataaat tatctgctat tcatgactcg agcataagta gtgatttatt ggataacaat
     1561 gaccataaaa atgaagatgg gataattaac atactactta tgggaacaga tgctagacca
     1621 aatgaagatt cttcaaggtc agatgctatg atgatactta ctattgataa caaacacaat
     1681 gatatcaaac ttacatcatt agctcgtgat agctatgtag acatccctgg tcatggtaaa
     1741 caaaaactta ctcatgctta tgcttatgga caagctgatt tattaataca aacaattgaa
     1801 gagaatttta atattgatat tcaaaattat gcttgtgtta atttcgaatc atttatgtat
     1861 ataattgatg ctattggtgg cgttgaggtt actgttgaaa aaggagaaat acgagaacta
     1921 aataaattta ttcctgaaac ttataaatgg aataaaagtg atgataaagg tagcatacaa
     1981 tacattagaa actctggaaa gcagactcta aatggatatc aggcactatc ttttgcacgt
     2041 atcagacaca acgatacagc ctttgctaga gatggcagac agagacagat tatacaagct
     2101 ataatcaaga aaacggaaac tttacctgtt actaaatatc caggtttatt agatgcagtt
     2161 cttccatatg ttaaaactaa catgaagcct aatgcaatac tttctttagg agctcaagtt
     2221 ttaaaaatgg gcgacttaaa tattaagcaa tttgaatttc ctattgatga tgaaattcat
     2281 agtacaggtg gtatatatgg aaaagctgga tgggttctta gatttgaccc agatacttta
     2341 gacatattac acgattttat atttaatgac atagagttta aacaataaaa aattcaacat
     2401 aaagtttatt aaaaagtata agattaatta ctaaaaaaga actatatcgc ataattattt
     2461 tcaagttgct gtgtacactt gattacaaaa tgcatatagt tcttttttat tttctaattt
     2521 atcatgataa ttttcatttt aatctacatt tataaaatac tctgtatttt aaattaactt
     2581 ttttattact taactaagtt taagaaagtg tttaatgtgt tttgatttat accatctcca
     2641 gcttcgtata ctagagtagt tttcttaggc tctaaagcag ttttctgagc agatgttaca
     2701 ctatttccaa gcataactac tggtgatttt aatttagctg ctaaaggacc tgatgttaat
     2761 gcatctacca attgattact tttgcttaca aacattgatg aatattcagc atttggatag
     2821 aatttttgta ttacttttcc atttgtttct tctctatttt ctccagctat tctattttta
     2881 gatacatcat ttgtaatgac cttatccagc ttatttatta ctgaatcaga tatacttaga
     2941 ttaccaccta taaaataagc atcttttact tttaaatctg atatccaatt gtaaacttca
     3001 tcacttacat tatcttttga tactaatact attggttgct tttcttctcc tgcttttgaa
     3061 gctatagaaa gagaatctgc ttcacctgta ccacttgtta tatatagttt ttctactgga
     3121 ttagttttta ctaattcttt tgctatcatt aaagatgtac tatatctatc tgaaccacca
     3181 actctattta ttgatacatt tgacaatgta tcttttatct cactctcaac tttatctcca
     3241 atagagttat ttcctccaat taaaataact ttacttggat taagtctttt taattcattc
     3301 ttagtggatg taggtatatt atccttattt accaatagta taggagcatc atttgatgta
     3361 gcaagtggtg ttgatgttat accatcaact atagagtacc catttactaa tactactgta
     3421 tctgaatttg tagcccaacc ttttttactt attgctactg ctgtgtcata tctttcgtcg
     3481 cctcttaatt tctccacttt aacattagat ggtggagtaa ctggttcttc actactagaa
     3541 tataaactct ttaaatcctt ttgtatagaa gtactatttg ctatatcttt agctgagaaa
     3601 tacatactac cttttatttc actatacttt ctatttaatg ttacttgttg tactatttct
     3661 tttgctatat tttgtccacc ataactcgat tgaccttgct tatatatacc ttgtcctatg
     3721 tataagtcta catttgttcc ttttacttca ttagcccacc atgccactaa tttagaataa
     3781 tctgctgctt taagaccaat tggccagtaa agttgtggaa ctacataatc aatcaatcct
     3841 tgttttatcc atgctcttgt atctgcatat gtacctacat aactttcatt acctgatgta
     3901 tctgaacctg ttgggtcact actcttattt ctccatatac ctgctgggct tacaccaaat
     3961 acaacattag gctttatact ctttatagac gcttttacat ctctaagtag agtatttaca
     4021 ttttctcttc tccaatcatc cttattttgt ccttttccat atttttggta tgttgcagta
     4081 tcattgaaac ttactcctgg atagaagtaa tcatcaaaat gaactccatc tatatcgtaa
     4141 ttttgaacta cttctgctat actatccact atgtactttc taacttctgg taaacctggg
     4201 tcgtagtaat acttatttcc atgtttaact acccagcttg gatttttctt tgctggatga
     4261 tttgctggca atttatctat gctctcatct gccatagtta ttctatatgg attaaaccat
     4321 gcatgaaatt ccattcctct tttatgtgct tcttctatta aaaatggtaa tgggtcatat
     4381 ccagggtctt taccttgtgt accggtaaga tattcagacc atgggtttat atttgattta
     4441 tataaagcat ctgattttgg tcttacttga accactgctg tatttattcc tacacttttt
     4501 aatttgtcca ataaatctgt gtattccttt ttttgcttgg cttcattatt cttagtctta
     4561 ggccagtcta aattataaac tgtagatatc caagcagctc tcatttcctt atcatttgat
     4621 gaatcagcaa atgaacttac actacacata gtaagtgtca ttatcaatga taatactaat
     4681 atacttattt ttttcatttc atccccctta attttttaaa tttatttttt ctacaatata
     4741 cttcttatta agtatatcta aaattcatat tatctgtcaa atattgaata atatagttaa
     4801 aatttatatt tgatatttga ttataatatg aaataattta ttataaaaaa aacagcaaag
     4861 aaatttatct tctttgctgt ttttataacg ttcatcacta ttttaatatt actaaactct
     4921 taaatctcta tgtaaaacat taaatgtaat tatatattta atgttaatat tcaatcctat
     4981 gtaaatcaat atctattatt attgtaaaac acgtgtagca gttataagtc tttttgcata
     5041 attagttcct ggagccatgc tagtaacttt tactacatct cctgtttgtg gtgaatgtat
     5101 gaatttagaa tctcctacat acaatccaac gtgatttatt gaaccacctg aaccaaagaa
     5161 aactaaatct cctggtttta attctgctct tgatactttc tttcctactg ttgcttgtga
     5221 tcttgatact cttggtaaat taactcctgc tccattttta tagacataag aagtgaaccc
     5281 agaacaatca aaagcatttg gtccagttgc tccccattta tatggcttac cttgttgctt
     5341 aaatgctaag tttaaaacat tttgtacctt tgcatctaca tttccacctt gaatagttaa
     5401 ataatctcca tttacccatc ctgtaacatt gtcaacactt tttattttaa tccatccatt
     5461 tgaatgtaat tctaaaactt caactttgtc tcctgtgttt atctttccta ctactgcatc
     5521 atttgcacta gctccacttc ttacatttaa aaaatcacac tggtctactg ttgcactcat
     5581 aggtgaatta gttggctctg ctgcactagc cgttattgct cctgcttcta ctgtgacaac
     5641 tcctgctatt aacaccatag ctataaattt tttcaatata tgttttttat ttttaatatt
     5701 catcataatc tcaaatacct aatcctttcg aaaatttaac attttgttcc ttcgtttcca
     5761 attcctaatt aacattctat cttacatcaa aataaattgc aacctttttt gtaatctttt
     5821 tgtaatcttt ttgtaatctt ttgtttcttt ttagtatata tttgtttctt tttgctcccc
     5881 ctggttacaa attagaaata tattctactt ttctctagtt gttgcaattt ctttgatatt
     5941 agcatcttct ggtttaatat aagttggcac taattccctc ataataccat ctattttttc
     6001 aacctcttca ttgttagtaa tttctcttaa aagttttaag tattttgtta ttttttctct
     6061 atcaacatct attggtctac caataaaaat ctttttatgc tctgtatcca acagaccttc
     6121 ttctgacata agtagttctt catacaactt ttctcctggt ctaagaccag aaaattcaat
     6181 ttttatatca acatctggct caaatccact gaatttaatt aagttttttg ctaaatccaa
     6241 tattcttaca ggttctccca tatcaagtac aaatatttct ccacctttag ccatagcccc
     6301 agcttgtata actaagccta ctgcttctgg tattgtcata aaatatctaa taatatctgg
     6361 atgagtaact gtcactggtc ctccatcttc aatttgcttc ttaaaaagtg gaattacact
     6421 accattacta ccaagcacat ttccaaatct aacagcaaca aattcagtct ggctttcagc
     6481 attcatagtc tgtataatca tttcagcagc tcttttagta gctcccatta tgttagtagg
     6541 gtttacagcc ttatctgtag atataaggac aaatttctta gctctatatt ttgaagacaa
     6601 gccagctatg tttaaagttc caaatatatt attctttata gcttcgccag ggctcgattc
     6661 cataagagga acatgtttat gtgctgctgc atgaaatacc acttctggtt tatatttatt
     6721 aaatatttca tccattcttt tttcttctct tattgatgct attactgttt ttaaatctaa
     6781 tttattttta tattttctaa gtaattcttg ttgtattgag taagcattat tttcataatt
     6841 atcaactata ataagctctt taggattaaa tcctgcaatc tgtctgcaaa gctctgaacc
     6901 aattgagcct cctcctccag ttattaggat tactttttcc tcaatgtaat tacttatctc
     6961 tcttaaattt gtttttatag gctctctacc caacaagtct tctatctcta cttctctaat
     7021 ctgttttata tcaacttttc catctataat ttcgtaaatt cctggtattg tctttatttt
     7081 acagttagtg tttttacata catcaataat ctcttttttt ctacgtttct ctatgtttgc
     7141 tattgaaaag attatctcat ctacattgta cttttctacc acatctttta tatctgaagt
     7201 tgttcctaca attttcacat tatggattat tcttccaatt ttatttttat catcatcaat
     7261 tatagccact gctactttct taagttctgg atttcttttt agttcctgta taaccatttc
     7321 tcctgcatct cctgcaccaa ttataaggat atttgaaacc tttcctctag aatatgtctt
     7381 gttcatgact attcttatag ctctataggc tattcttgcg ccacttgtaa acattataat
     7441 aaatattgta tttaatacat aaaacattat aggataatca aaccctaata atttatttat
     7501 tatataaatc ggaattaaat aagctaaagt tgccacaaaa atagatataa tctcttcttc
     7561 tcctgcatat ctccataatg tgttgtagca cttaaacaat ctaaatgata ctatatgaag
     7621 tactatatat atacatactg aaattattat atcattagtg gtaaatgcat ttaaattacc
     7681 tgtcaagctt aaagacattc gaaacgctac tagtacgcta caaatatcta gaattaatag
     7741 tgttaaatgt ctaaatttat attttttaaa aagcctatta aaaaagcttt ctttctctaa
     7801 atctatactt tctcgcataa ctagaagccc tcctttaaac agcaaaagct acgcacctaa
     7861 gcatagccaa caacatctat cttttgctgt attttttcat tttatttttt atatttatca
     7921 aatatagagt cataaatttt ttctatatca ttaaagttta aattaacctc ataattttct
     7981 ctaacaaatt gacgcccttt cttacccata ttaagtctaa gttcatcatt ttccattagt
     8041 tttattatag cattttttat ttcttctggt tgttttggtt ctacaattaa gccagtttca
     8101 ttttcccata cagattctgg atgtcctcct acatttgtaa ctataacagg tacttcacat
     8161 gcttctgatt ctatagctgc cactccaaat ccctctctta aagaaggaaa tacagttaca
     8221 tcaaaagaat taaaagtctt gcttacattt tctggagata ttcttcctaa aaactgaaca
     8281 tcattttcta tacccaattc tttagctaaa tttattaggt tgtccatctg acttccagag
     8341 ccacctattt taagaattat tttcttatct ttatattcat ctttaagcat cttaaatgct
     8401 tgtattaaat attctattcc atattttttt tctaaactct ttataattcc tattacaaaa
     8461 gcgtcttctt tttctacttc cataggctta aacctatcca tatctactcc aaaaaaggtt
     8521 acatctacat gtttagctgt atatttatta gcctctttag ccatatctct actatttgaa
     8581 aagatatagt ctgcttttct aaagttatgc tttataattt gtttttgtat aaaaccatta
     8641 ttagggaaat cataaatatc agtaccccaa acagaaataa tatatggatg atacccaagt
     8701 agacttccta taaatccata actacttgca tactgtgcat gcaatatatc tggctttatt
     8761 tcatttacta gcttctttat ctgctttatt gaacctaagt atcccgtttt tttagaaata
     8821 ttttcatttt ttagttcttc tacattagaa ccaaaattat aaatttttat tccttccatg
     8881 tgacctccat ttaaagagat tacatgaatg tcatatcctt tatttttaaa aaaactacac
     8941 cattttaaag tatgaggatt tgacccatca gctaaataac aaacagtctt cataatattt
     9001 accttcctat cgaataatac tcaattccta actcttccat atctttaatt ttataacaat
     9061 ttcttccatc aaggactaaa ggatttttca tcttttcctt atacttatgt atatcataat
     9121 ttaatacatc tttccattct gtcattataa aacatatctc tgcatcttct atcgcatcat
     9181 ctacagaatc agcataaagt atagagtcac caaatatttt cttagtatta tttacaccaa
     9241 ttgggtcgta tacagtaact cttgcccctc tttcgagcaa atattttata tttggtatag
     9301 atggtgcttc tcttatatca tcagtgtctg gcttaaaagt caaacctaaa acagcaacct
     9361 tcatatcttg aaagctctct actaacttag atgcctttct aactaattta agtttttgac
     9421 tttcattaac ttctattgca gcttttactg tttttaattc ataaccatta tgttcagata
     9481 accagtgaag agcttttgtg tcttttggaa agcaagaacc accatatcct attcccgcct
     9541 ttaaaaactt acttcctatt ctatcatcat aactcattcc taaagctaca tcttctatgt
     9601 tagctcctgc tatttcacaa agatttgcaa tatcattcat aaatgatatt ttaagagcta
     9661 agaagttatt tgatgcgtat tttatcatct cagcactatt tctatttgta actattatag
     9721 gttgattaaa aggcttatag attttctcta atatttctct agcccattca ctttctacac
     9781 ctataacgat tcttgaagca tgtagagtat cttttactgc tgttccttgt gataaaaact
     9841 ctggattaga tgcaagttct acttttacat cattttttag gtttctattt atataatctt
     9901 ctatctcttc atttgtacca attggaactg tagattttat tactactaga gtatcttttg
     9961 tcacagtttc agctatttgc tttgcaactg cctttatata atctaggttg gctgagccat
    10021 cacttctctc aggtgtacct acagctatta atattacatc tacatcttca taagcttttt
    10081 tatagtctgt agtgtaatct attcttcctc tgttgtaatt tttaaccata agttcctcta
    10141 ggccttcttc atatataggt gctattccaa gtttcattga tttaactttt tgttcatcta
    10201 catcaacaca gactacattt tcattgccta cctctgctag acaaacacct gtaaccaaac
    10261 ctacatatcc cgtcccagct atagctattt tcattttttt acctccaagt atataaaact
    10321 tatactcttt ttttaatggc attaaaaaca tagaaacaaa atacatatag actttttggt
    10381 aaacttaaat tttcaacatt tctataagta ttccaagttc gttttaatgc ttttatcttg
    10441 ttacttgata tagaatttcc aactaatctg taattcacta aatcttcttg aatcccatat
    10501 gcatattttt cctttttaag taactgtaac caggttgcag tatcttgtcc tcttcttacc
    10561 aaaggcatgc tgaactctcc tacaatctcc ttatcaatga ccactgtaga acatcctatt
    10621 atagtatttc taagtaatcc ttcatagtca attttcttag gagctcttgc aaccttattc
    10681 gtttttgacc catcttgtct catatatcta taagatgtaa aagaaaaacc tacattttct
    10741 tttaacatat attctatctg aatctctaat tttctactat cccataaatc atcactatct
    10801 acaaatgcaa taaatcgacc ttccgcattc ttaataccaa catttctaga tactgcagct
    10861 ccagaatttt tttcaagctt tatatatttt attctagcat cttcttttcg aaaacttttt
    10921 ataatttcac ttgaattgtc cttagaacaa tcatccacta ataaaagctg ccaattttta
    10981 taagtttgat tttggattga ctttatcgtc tctgataaaa actcttctga attataaaca
    11041 ggagtaatta tcgatactaa aggctcattc atatttttac tctccttata tagcaactag
    11101 cagtactata ccaacaagtt tacttagata ctaattttct ttgtccaata tatttgtaat
    11161 atcctaatgg gaataatgct aaatacaaca atttatcaga tacttttctt aattcaccaa
    11221 aataatgact atcagacaac aatgagtatc tggctaaatt tatgtaattt tttatccaca
    11281 tatatggata tctacttaat ggatatgtat tttgatttat taaatataaa aactctaatg
    11341 ctctacctct taatgcattt ttacttaata tattcttagt caaaccatca tccaaataat
    11401 ctcttattat cataggttta tttacacata taactttata gtatttagac atttcattcc
    11461 atactacact ttctggtaaa aatctcactt cttttctttc tggaaaagga aattttataa
    11521 gctcatcagt tacaaatact atgcttttat ctcccttaac gccatactta aaatataggt
    11581 ctgcaaagtg acatattttc ttatcttcag gcatttctgt ccctatcaaa tctccacttg
    11641 gatatacaca aagtccaaca actccactaa atccttcttt attctctaaa gaatcccact
    11701 catcattagc aatttcaaga gaatctttaa ttaaaccatc atcactatct acaatgaaaa
    11761 atagctctcc atctgcattt tttacaccta gatttattgc agtatgtttt cctccatttt
    11821 ctttgtatat atatcttata tttaatttat tttctgatat aaatttttca actaactctt
    11881 tagtactgtc gctacttcca tcatcaacaa tcaaccattc aaaatcattg aaatcataag
    11941 tttgagcttt taaatcattg tacaaatttt caagtaaatg agctctgtta tacgtcggag
    12001 taaatacagt aaatttatat ttatacatta attaaatctt cctctctgtc taactttttt
    12061 tgattccgtt tattccataa ttaataaagt agtatatagc tttgtataaa ggtatttttt
    12121 ctatatttct atatatattc caagtccaca tagcagcttt tattttatta ccacttaggg
    12181 aattactaga ttttctatat aatgctagaa cttcatttat tccatgagca atatgccctt
    12241 ttttcaatat cgataaccat aaaacataat cttcatgtct tacaccactc attcttattt
    12301 caaaatccaa tttagactta tcaatcacaa cagtaagaca tcctaagata tttcctttta
    12361 ataatcttct ataatctaca ttaggtggaa cttttattac tttgtttaat tttttatcat
    12421 tttcatccat cagttcatat gaagtaaatg aaattacata gtcattttct aacataaaat
    12481 ttacttgttt ttctaactta ctactattcc attggtcatc actatctaaa aaagctataa
    12541 attgtcctgt tgccttactt agtgctaaat ttctagcatt agagacaccc ttattagtct
    12601 cagtctttat acattttatt ctactatcct gttgcatata agatttgact atattaggac
    12661 tattatctgt tgagcaatca tcaataatta acatttccca ttcttgataa gtttggttta
    12721 atactgattt tatggttgct tcaataaatt tttcagaatt atacatggga gtaattatag
    12781 atactaaatt cttcttcata ttaccttcct catcatttat aaaatattta tttaatttta
    12841 cttaatacac aatcatatat cctcttgcag ttcttattat cattatatct gaaaaactta
    12901 tttctttcat ttttttggtt atccataacc tcaaaattat tttcaataat atcaataagt
    12961 ttattcacgg cttcatcatt attataagcc aaagacccaa acatttcact ttttaaatct
    13021 atatatgaat caactttttc catatattca tccaaatcga attggtaaaa caagataggt
    13081 ctattcatat acaaaaagtc tatggctaca cttgaataat cagttatatt tgcagctgat
    13141 tttctgattt catttgctag agttacccct ttatctaaaa acacaattcg ttttcctgtt
    13201 atattttctt ttatatcatc tataaactca tgcataagat gatgcatata aatttttaca
    13261 tttatatctt tctcatctaa tattttattt aatttgtcat catttaaaaa attcattata
    13321 tttttaaaat aatctgtatc cgtaaatttt cctgaactaa attttatcca atctctccat
    13381 gtaggagtat ataatatatc tcgagtttgt gcaacttcat ttgttggtaa attgtcatat
    13441 cttgcatttc caattatata aactgagtca tctaacattc cccaatcatt taccataaca
    13501 tttctttcaa attcagaact tgctgtaact atattatagc ttttatatag gttatctata
    13561 atatttttga actttttgtt cataggtttt ctttttttaa aacttatagt cccatgattt
    13621 aaaaacactt ttatcgtttt tttatgaaaa taattaagaa caggtacagc aaaattgtat
    13681 ggtgctatat ctgctgatgg tgcatgtgaa aatactatag cttttgaatt gtaataatac
    13741 agatagtttt ctacacttcc tcttattaat tttttacctg gaattttatc aaatacttta
    13801 ctgtctttat ttactaccca ataagtttct acatcattat gttcttttag catatactca
    13861 taaaaaaact ttgaattatc attgtatata tcccctgaat gtccacctat aagccagata
    13921 tctctaccat taaacttatg cttattaaat ggatatataa aaaaagccaa aagcatattt
    13981 attaaaaatt taaccttgtc cataaacacc atcctcatta taacttgtac tgtctaaatc
    14041 cattaattct tccttttata gtggcactat gataatctct attggaactc aatataagac
    14101 ctcctatatt tcttacaagt tttgatacca taacttttgc aaacttataa ttttttctat
    14161 agactatttc ctttttgcaa agcgctccaa agcctcttcc atagttataa tctttttgat
    14221 acttttcttt agatttagaa tgtttttttg caggatggta aattatatca tttccaaagt
    14281 attttccttt aaaacctaaa ctcaataagt ttaaaacata atctatctct tcaccagcac
    14341 caaactctcc accaacaccc aatttttcat caaatcttgt atagttttta ccttcaaaat
    14401 caataaaaaa agttatggat gttatagtat ccaaaacgtt ggaacttgtg atatcacaag
    14461 ttccatcata cattttctta aatgcatcaa ctttatttga atccatagtt ttgcaactgt
    14521 atattttata atctttattt ttattaaaga aatttataac tttttctaat gtatcatttt
    14581 cgtagacaca atcatcatca gggaaaccta taatttgccc tacagcattg tctatgccta
    14641 catttctgtt ataactcaat ccttttttag agctttttat atactttata tctaacttat
    14701 caatgtattt atcaactatt tctttaactt tgctattatc attttggtct actactataa
    14761 gttcaaagtt tttgtaagtt tgattttcta agctgtccat aagtaagtat atatcatcat
    14821 atctatttaa tgttggcatt attaatgata ttaacattac tacctcctac ttttcatctt
    14881 ccacaaatct atttcccttt tatcatttaa tatcacttct tttattagta atactactgc
    14941 aaaactaaat gaagcttcta ataataaatt ttctgcaaaa gagtatagta aaaatataaa
    15001 taaagctact aaatatgctt ttttatcttt ttctataaag ctttctattc ctttatacat
    15061 caaatacata aataatatac aagcaactac tcctagtaca ctaattatat atacataaga
    15121 actatctaat ggattagttg cttttatagc aggactatat gaatttccaa aaggttttag
    15181 aaagttcccc ttttcagcca agtatacatg ccagaactta ggtctacttg ctaataatct
    15241 atttaataaa gtgcttttat caaatactgt accaataatt acactaaata aagtaagtat
    15301 tattggtaat gtctttagga gcattggtag tccttttatt ttttttatat ctatccatct
    15361 tattatttct acaaatatta atcctgctaa tatagtgaaa aatccagttc tactatatgt
    15421 ggtttgataa acaaaaaatg cagaaccaaa taaaattatt ctatcccatt tattataatc
    15481 tttatatctt aagaatatat atgctgcata tatagtaacc atatgataaa atacactatt
    15541 tggattacca agtccaaaat ccattcttgt aacaagcata tctatttttt ctatatatct
    15601 atagtggact tcctgattaa atattaatga aggaaataga ttaaataata atatagctac
    15661 cattaaaaaa catgccgata ttattaaata atttttaact ataaatttat catctatttg
    15721 cttagctgat acagctaata aaacgattaa taatatagct ccttccctat ttaataaata
    15781 aagtaagaca caaactccaa ctagtaccat ttctgttata ctttttctct tatcaaataa
    15841 aaaaagtagc attagtattc ctattgaaga ataaaataac gctgatttaa tcatccctat
    15901 atccatattt gggtcaataa atgatatcat atactttcta agtaatatga taaacaaaac
    15961 aaatatataa agatattttc taatcataat atttctatca tcatttaaag tcttcatttt
    16021 actttctccc ttttagcttc gtaaagtatt acttgcaaat attttacaac acttccatat
    16081 cttatttaca cctgttaagt gtttgctttc taagaaataa tttctctttt tagcccaact
    16141 ttttccccac caatgtatcc caaaagtgtt ttctgttata cattctagtg taaagtcttc
    16201 tttaaatcca aaaggataaa aatattcttt gtcgtaaaca tgtattcctt cttcataatt
    16261 atttgggtca atcttcttat aattcttttc cataagataa gtcattatct ttggtattgt
    16321 aaacaatgaa gaagtaagta cttctttttc atagtactcc aataagtctt ttaaaaaagg
    16381 atgtcctttt tctacaccta ttatggcacc atttatatac tctctatcat cttcataccc
    16441 acagattagc ctattatttt ctaaaagagg agttatatcc tttaatattt gcatatctgt
    16501 atccatataa actccacctt gttcatagag tacttttatt cttgtataat cagatatgaa
    16561 tgcccaaagt tttcttttat aacattcttc caaaaactta tttcctttta tttctttctc
    16621 tatatcaaag tttttttcat tccactctac tatctcatat tctggaagct tttctttcca
    16681 agaatttata catatatttt ctatatttcc ttttggacca ccaaaccata cataatgtat
    16741 ttttttagga atcaaatgaa cacctcctac atatattctt gtctatcttc ttcttttaac
    16801 tgctctacta tttttttgac atcttgagct ttatcttttc tacaaaccat aactgcatca
    16861 tttgtagata ctactatcaa atcactaata cctattgtcg atattaattt gtcatctgaa
    16921 tatataattg aatttttagt atctatagta atattttcac ctcttttgat attaccttca
    16981 tcatcagttg gatatattgc acctagagaa tcccaagaac caacatcatt ccatccaaaa
    17041 tcaccaggaa ctactataac atcattactt ctttccataa taccataatc tatagagatt
    17101 gattgtatag ttgggtatat ttcttttatt ttttccattt cttcttttgt tcctaagtat
    17161 ttacttatat cttctaattt ttcatatact tttggcaagt atctcttaaa atcttctagt
    17221 attttagata ccttccatac gaacattcca ctattccata catacttttt agagtttaca
    17281 tattctttag caatttcata gtttggtttt tctacaaact ccactacctc atatgcaaca
    17341 tcttctatcg tattttctct attaaaattt atgtatccat atcctgttga tggaaatgta
    17401 ggagttatcc caatagttac tagtttatca ttattttctg ctatatagat agctttttcc
    17461 aaaatagatt taaattcttt ttcatctttt atataatggt cagctggata tacacacata
    17521 acaccatcac catatttttt cattatgtta aatgctgcaa agcctatagc tgctgctgta
    17581 tttctggcac atggctctgg caatatatta ctatccaaac atttatcttt tactatatcc
    17641 tttaaagctt ccaattgctt ctcattggtt actataaata aatcatcttt ttttgctaga
    17701 gaatctattc tgtttatagt ttcgtttatt aaagcatctt ctccacttaa atttataagc
    17761 tgtttaggta cttcttgtct actaagtggc cagaaacgag ttcctcctcc tccagccatt
    17821 atgacattgt atactttcat tgtttatctc cccttagctt tttatataga atccactaca
    17881 tttaatatat aagatattaa attgttcaac ttaaattcta catcttcttg attgtctgaa
    17941 tttgctccaa agtaaaattt aattttaggt tctgtacctg aaggtcttat agctatccaa
    18001 gagccatctt caagtaaaaa tttaagtaca tctgacttag gtaaatcatc aacaccatct
    18061 ttatagtcta aaattttatc tacttttaca tcagccacat tatctatttt aacacttcta
    18121 aagtaaagca tcatctcttt tatttttttt attccatcta tgccttttaa agttactgac
    18181 tttaagtcct ctttgtagta tccaaattta ttatatgtat ctatcaaagc ttcatacaag
    18241 ttcatacctt ttgaagaata gtatgctgcc atttcacata tcataagtga agatacaaca
    18301 ccatctttat ctctagcatg agttccaatt aagtaaccat agctttcctc atatcccatt
    18361 ataaaacttc tatcattgtt ttcttcaaac gcttttattt tttctcctat aaatttaaat
    18421 cctgttaaag tatttaaaca atctacatta taagcctttg ctatttttgc acctagttct
    18481 gatgtaacta tagtttttat tattgtagga ttgtttttag gaagtttatt ttcttcaact
    18541 aaactctcta atatatatct aactaaaagt gaaccgactt gatttccatt taagactaca
    18601 tattcaccac ttgaatcttt tacaacgata cctactctat cacaatcagg gtctgttcct
    18661 attatcaaat cagtgccatt attctttgcc atttcaatag ctatattaaa cactgacttt
    18721 tcttcaggat taggatactt tactgttgaa aaattagaat ctggcaattc ttgctcctta
    18781 actaccatta cattttcaaa gccacattca tttaagactt ttcttactgg cttatttcct
    18841 gtaccatgaa taggtgtata tattattttt aattttttcc cgtattcatc tattatattt
    18901 tgtcttaaaa cttgcttctt aacagctttt ataaactcat catctacttc attatctaat
    18961 atagttatta aatttttaga taaagcttct tcaaaatcta tacttttaat agtactgtaa
    19021 tcatcaatct tattaacttc tgctattata tcatttgcca tatctataca gatttgacca
    19081 ccatctgagt catatacttt atatccattg tattctttag gattatgact tgctgttatt
    19141 actattccca tagcacactt taaatatcta acagcaaaag aaagttctgg tgtaggcttt
    19201 aattcttcaa aaatataagc ctttacacca taagcactta aagtctttgc tacttctata
    19261 caaaactccc tagacatgta tctactatca tgtgctataa ctattccctt ttgcttacct
    19321 tcttctcctt gagttttcat gatataattt aaaacgccca gtgtcgcacg tctaacagta
    19381 taaatattga ttctattagt tcccgctcct attatacctc ttaaaccacc tgttccaaac
    19441 tctaaatttt tatagaatct atcttgtatt tctttttcat catcttttat acttaatagc
    19501 tcatttttcg tttgttcatc aaaataagga gaatttaacc acatctcata attattctta
    19561 taatccatat tttcatcctt tctgactcta atttaaaatt tcaaggtaat tatctatctt
    19621 ctaagtagtt tttgttttcc ttttcttaaa aattcaaaag ctaaatccat ctcttctacc
    19681 ttaaataaaa ctatcaatac agtatataca gaagctccac ctaatactcc agctccaact
    19741 gatataattt ctttaattgt tcctgaaccc ataaatgcat atagattatt atatatcaat
    19801 aaagtagcta ctgccatcac accagatgct accaaacttt ttagaccagt ctttataatt
    19861 ttatcccctc caaaatatcc atttttcttt ttaagagata taaataataa tataactgta
    19921 attatgtttg atgtactagt tgatatagca atacctgcat gacccaatgg tcttatcaat
    19981 attaaattaa gaactatatt tattattaaa gctatacttc catttatcat tggcgttttt
    20041 gtatctgaaa gagagtaaaa tgccctatat aagacatctc ttactccaca agccaataaa
    20101 cctagtgaat ataatctaag cgctatagat gtaaggtctg ttgataacgc atcaaattta
    20161 cccctttgga ataatattct aacaagaggc tctgctaata taatagctcc tatagatata
    20221 ggaagcacca ccaatataat acaatttgaa gattttacaa tagtgcttat aaatccttct
    20281 ttattatctt taccagacat ttttgctagt ttaggatata taactgttat tatagatgta
    20341 acaaataatg ccataacaaa ctcatttagc ctatttgcat agttaagagc agacaacttt
    20401 ccatcaccca aagtggttgc caataatcgg tcaacaaata tatttaactg tccaacagct
    20461 actcctacca acataggcaa tactaggttg actaactctt ttatagactc atcctttaaa
    20521 tttatatatg gcttatactt gtaggacttc ttaaacgcaa atggcagttg aaacaatagc
    20581 tgactcgcca tagccaataa tgctcctgct ggcagtatcc atacattttt acctgcactt
    20641 agtgctattg ctgctattat tataatatta tatgggattc ctataagccc aggaattaca
    20701 aaactgtcat tcacctgtaa aaacgaacta aatatcttac ttccactcag gaataaaact
    20761 cctgaaatca ttattttagt aaataatatt gctgcttgga acttagcagg gtcgtttcta
    20821 aatcctgctg caaatactgt aacaaattgc tctgtaaata aaagcccaat aatagctatt
    20881 actatagcca ttatatagca tatatttaaa acattattag taaattttaa ggcaccttca
    20941 tctccgagtc ttttctttgt ctcaaagtac ataggtatat aagttgtagc aactgcagag
    21001 cctatgactg ctatgattaa accaggtata tttaatgtta atacaaatac gtctgcatac
    21061 atacctgtcc catagaaatt tgcaagtaca agttctctaa ggaaacctaa tacttttgaa
    21121 aacatagtgg ctgccataat ccacaatgct gctttagctg tttttgacat cttctttctc
    21181 cttatctaat ttaaactcta aaagtagaac ttttaaaact gcttataaac attattctaa
    21241 ttaagtaaat ttaacctatg aaaagcttcc ttataaatca aaaactattt cgactcatat
    21301 ctaagtgccc taaatgtaat caatagtaat aatagtatta atactataca agataagaat
    21361 gttagattaa atatgctctt aaatagacta taatcaccta ctgtataccc aacttcaact
    21421 acatcattta tattgctatc tttcaagaat ttatcttgtg cacgacttaa gttatcacta
    21481 caaatcagta ttggcatatt ttcttttgct gatactactc ccaaagaaat atattcgttt
    21541 acatcagatc tactaccaaa gtctaatcta gcagtataga ttttctttat atctttttca
    21601 tcataaaact tctttattaa atcaacattt gtacttgact tatttatttg ctttattcct
    21661 tctatatttt ttaagttaga atcaatctct gcaataaact cttttgaatc acctaatgca
    21721 tatatttttt taagattctt gccttttaag aaatcaattt gttcctcaaa cccttcatct
    21781 ttagcccata ttattggcat tccactttta gcggcataag aatatacaga tatggcgttc
    21841 tctaatcctg ttgttgagct aagtaaaaat gcttcatcaa atcctactgt tctatacatt
    21901 tctctagcta tctttaaaga tgtatcatat ctgtctttcc ctcttattct ttctactttc
    21961 attcccattt tttctatatt acgttcagca tctttagata ctgcctttaa accacctact
    22021 atcgtaacct tctctggacc tagttctttt agataatttc ttgttactct accaagattt
    22081 ttactcttag ttacaactat tggagcattt tttgcatatg ctaaaggtgt tgcacttata
    22141 ctatctacaa ttgactcttc atttacaaga attacttctt tagaatcctt aaatgctctt
    22201 ttatttaatt ctgtcgaaat ctctggtata ctcccttcta tcaactctct tttgggattt
    22261 gcatctgcaa atactcctat cgtactagaa atagagatta acaaaactaa cgataatata
    22321 gtagaatttt ttttaatcat aagttctcat tccttttctt ttttgtattt tttgacaata
    22381 aaaaagctct ttttaaagag ctttttcatt gattagtaat ctaatatttt aaaattatct
    22441 agctccgtct cctgtcaata ctactttaac agttttaaag agtatcttaa agtcaagtaa
    22501 tatactccta ttttttatat agtaaatatc ttctttaagt ttttcttcag gagtgatttc
    22561 gtatccacca tttacttgtg cccaacctgt aagcccaggt tttatagcga gcctgttgat
    22621 aaatccaggt atttcctcat taaattgaac agtaaagttc ggtctctctg gtctaggacc
    22681 tattaagccc atgtccccct ttaatatatt aaacagctgt ggaatctcat caattctagt
    22741 tttcctaata aactttccaa ccttagttat tctaggatca tctttttcag cccattgtgc
    22801 gccaaatttt tctgcgtctg ttctcataga tctcaattta tatatataga atcttctccc
    22861 gcattttcct agtctttctt gcttataagt tattggtcct ttgtcctcta ttttaataag
    22921 aattccaaat attgctatta aaggtaaacc tatcacaagt cctattatag ataaaactaa
    22981 atccataatt ctttggtaca agtcgaataa aactgttcct tttactatgc tatagtcaat
    23041 ctcacttgca atttctttat ctgcccctgg atagctttta attttaggta tttcacactc
    23101 atcattagta tatccagaca tagttacctc catattggaa taaaaatttt tttaattctt
    23161 aatttcaact tatttctatc ttatttttga cttatttcta tattatactc aataatgagc
    23221 agattatcaa tacaagaaac atcattgtaa acaatttgat tctttacaaa aatatatcat
    23281 ctcttgttat attttacatt ataacaggag atgatatgtt ttgctttaat attttctatt
    23341 ttattttact gtatcttcta ttcctattcc aatttgagtt cccattgttt ttactttttc
    23401 tgcagtattt aacttatcca tatcgctctt attgtcaata aatgcacatt caacaagtat
    23461 tgctggataa ttattatttc ttaacacata taggtaatct tttccattat caagtgttct
    23521 tgtcttgatt cctctatttt tcatgttaaa tttttctagt attcttttta agatatttga
    23581 tgctgcagtc ttagttgttc caccattttt atcttttact ttgtaataaa tttccacacc
    23641 attaccagct ccattagacg cattgtaatg tatacttgta aataaatctg gctttatagt
    23701 atttgataat gctgttcttt ctcctaaagc cattgtttta tctgtatctc tagtcataac
    23761 aacatttata ccttttgaac gtaaatattc agtagttgct aatgctgtat taagagtata
    23821 tttcttttct tgtgctccac catttaatcc acttgttgcc cctgaatcag aacccccatg
    23881 acctgggtct atcactacag tttttcctgc tgctgaacct gaattatctg gttctgttgg
    23941 tgcattgtgt tttgataaac tagatgctat agaatttata acactatctt tcattccatc
    24001 accaatttga tatactgttt ctgctgtttt ttctgataag tttgtactat ggtatgcaga
    24061 aacataagtt tttggagtta taagtattgg agcttttaat ttagctgcta aaggtcctgc
    24121 tgttattgaa tcaactatta tgtcagattt tgcaactaac attgcactca actctttatc
    24181 tggatagaaa gtcttaatta catttgcatt tgtttcatgt ctatctgctc cagaaactct
    24241 attttttgaa gtaccatttt ttgctatttt agatatttgg tctataattt ttgatgataa
    24301 actttgacta ccacctatgt aataagcttc ttctaaacct tgtgaagata accaattata
    24361 tgtaccttgt ggtacagaat ctttattagc taatattata ggttgttggt cttcaccagc
    24421 ttttgatgaa atattaagag catcatattc accagcatat ccatttgcta tgtatatttt
    24481 attaacatca tgatacttat ctatttcttt tgctataagt aaagatgttt catgtctatc
    24541 aactccacca atacgagtca catttacatt tacagcagat ttaatttgag acactgcctt
    24601 ttgtgaaact gaaccatcat cacctataat tattacatca cttggattta gacgttttaa
    24661 ctctgctttt gtactttctg gtatatcatc tttatttgca agtaatattg gtgcgtcata
    24721 tgtagatgca agtggagtag ctgtaattcc atctgctgct aattctccat taactattac
    24781 aaccttacta gacccatctt tccaaccttg tttacttatc tcaacagcag tttcaaatct
    24841 agtagctcca gcaagttttt ttactggtgc agaagttttt ctcatatatt catttactgc
    24901 ttttgttgaa tttgcatcac ttattataaa ctcttgtgta acatcataat ccttaagatt
    24961 taatcccata gactctaagt cttctttgtt taaaactttc tgttcatctt taccatttat
    25021 ttttatgctt tgaagttccc atacatttaa gtattctggg tctttgttat agtttattac
    25081 accttcatat gaatctgtct tttcattgta atttaaagta gcttcaagtg gcatatcata
    25141 acacataaaa tttaatgtta ttgaatcagc atttaatttt tctttaaatt ttacacttgc
    25201 attagattta cttcctaaca ctacactttt tctttgtaaa tttatattaa tatctgaatt
    25261 agattctgta tttgcagtag tattttcagc cgctaaagct acatttggtg ccaccattgc
    25321 tacagacaac attcctgttg ccaataattt tgttgttttt ttcatcatat cccccctaat
    25381 tttttggttc tttaactctt taaatctttt ttaatacatc ttaagcttgt tttatattac
    25441 ataaattcaa tattatgtaa atcttttttc attatagcac aaaaaattac aaattgtaaa
    25501 ctttttgtaa tcttttatgg catggttttt aaattttatc gaggagaaat tttgattata
    25561 tttgatagtt tccgtatatt tgaacaaatc aaagacttta tcatctaaaa atattgcact
    25621 aaattttaat aaatattttt ttcttgtaca ttatagacat tactataacc cagaatagga
    25681 catacaataa agaaaatata agagaatccc aagttgtacc tgcccaaggt gttataagtt
    25741 catatgtagt ccactctacc aaggtagttg gataatcgac tttgttcgta agttttggaa
    25801 cattccaaaa aacatgactc aatatttcaa ggcacatata tgtaaaaatg gggctacttc
    25861 caagtgctat tattggggta aatattttac ttttattttt gatgtcgcat ataaaataaa
    25921 atattgacag taatatacca taactgccag ccattaaaag tacaaatgag ctagaccata
    25981 atcttttatt aaatggaaaa tactgattaa agataaaagc acatattagc aggattatac
    26041 tcataaccag tattttaaag aattttttat attctcctag attttctttt aaaagtacac
    26101 atcccatagt gcatcccaac atacctgaag aaattgctac tatactagtt aaaaaaccat
    26161 ctggttcaaa ttctggcttg tacaaatgtc ccttgaagaa atgtaaatcc actaactgtg
    26221 caagagaacc ttctagctca aatccataag gttttgctaa tatgtagtac ccaactatta
    26281 taaatgtaga tatacataga aaagtaatta ttgtagcagt acttccaaca tttaattttt
    26341 ttagtagtaa atatactaag ctagtcacaa aatagactaa tcccatacgt tgaagtactc
    26401 caagtattct tacagtatct aaatcaggat ttcctaggta atttaagaaa aatccaaata
    26461 atatcaataa gatacttctc ttgaatatac taagtattat actcagtgtg gatttattat
    26521 tttttagttt actatttatt gatattggaa ttgtaacacc taaagatata acaaaaaaag
    26581 gaaatgcaaa atctgcaagt gttacaccat gccatacagc atgtcttaat tgaggataca
    26641 ttctcatcca agtcccagga ttattgcaga ctatcatgag ggctattgat agccctctta
    26701 ttatatctat tgatttaatc ctactattcg taagtttaga atctacagca ttatttgaaa
    26761 tt
//
LOCUS       DAEMHW010000038        26664 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEMHW010000038 DAEMHW010000000
VERSION     DAEMHW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.038"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..657
                     /gene="yunB"
                     /locus_tag="KPK67_001369"
     CDS             <1..657
                     /gene="yunB"
                     /locus_tag="KPK67_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBG2458362.1"
                     /translation="KIIAIFLVILFLSVFIGSFIYIDKTLRPTITVLAETKALELANR
                     SINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIASAIALEIQDELKKD
                     KTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFESSGINQTRHRIYLE
                     AQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKNLGNVLPNTGKNTNK
                     "
     gene            complement(869..1876)
                     /locus_tag="KPK67_001370"
     CDS             complement(869..1876)
                     /locus_tag="KPK67_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG2458363.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            2104..2988
                     /locus_tag="KPK67_001371"
     CDS             2104..2988
                     /locus_tag="KPK67_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2458364.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            3190..4740
                     /locus_tag="KPK67_001372"
     CDS             3190..4740
                     /locus_tag="KPK67_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2458365.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            4769..5416
                     /locus_tag="KPK67_001373"
     CDS             4769..5416
                     /locus_tag="KPK67_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBG2458366.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSILLFVMYKLCKSLSIKIFQC"
     gene            complement(5558..5818)
                     /locus_tag="KPK67_001374"
     CDS             complement(5558..5818)
                     /locus_tag="KPK67_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBG2458367.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            6035..7318
                     /gene="hflX"
                     /locus_tag="KPK67_001375"
     CDS             6035..7318
                     /gene="hflX"
                     /locus_tag="KPK67_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG2458368.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNISYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIETALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            7326..8003
                     /locus_tag="KPK67_001376"
     CDS             7326..8003
                     /locus_tag="KPK67_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBG2458369.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            8089..8532
                     /locus_tag="KPK67_001377"
     CDS             8089..8532
                     /locus_tag="KPK67_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG2458370.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            8837..9481
                     /locus_tag="KPK67_001378"
     CDS             8837..9481
                     /locus_tag="KPK67_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBG2458371.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(9567..9944)
                     /locus_tag="KPK67_001379"
     CDS             complement(9567..9944)
                     /locus_tag="KPK67_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG2458372.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(9944..10330)
                     /locus_tag="KPK67_001380"
     CDS             complement(9944..10330)
                     /locus_tag="KPK67_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG2458373.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            10509..11264
                     /gene="nadE"
                     /locus_tag="KPK67_001381"
     CDS             10509..11264
                     /gene="nadE"
                     /locus_tag="KPK67_001381"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBG2458374.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            11347..12066
                     /locus_tag="KPK67_001382"
     CDS             11347..12066
                     /locus_tag="KPK67_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2458375.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(12233..13237)
                     /locus_tag="KPK67_001383"
     CDS             complement(12233..13237)
                     /locus_tag="KPK67_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG2458376.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQIIYPSIIYRQSSPAQNLL
                     "
     gene            13503..14399
                     /locus_tag="KPK67_001384"
     CDS             13503..14399
                     /locus_tag="KPK67_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBG2458377.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNSYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            14537..15799
                     /locus_tag="KPK67_001385"
     CDS             14537..15799
                     /locus_tag="KPK67_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458378.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPAFAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            15875..17068
                     /locus_tag="KPK67_001386"
     CDS             15875..17068
                     /locus_tag="KPK67_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG2458379.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKAIKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            17697..18491
                     /locus_tag="KPK67_001387"
     CDS             17697..18491
                     /locus_tag="KPK67_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG2458380.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            18586..19926
                     /locus_tag="KPK67_001388"
     CDS             18586..19926
                     /locus_tag="KPK67_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG2458381.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            20063..21295
                     /locus_tag="KPK67_001389"
     CDS             20063..21295
                     /locus_tag="KPK67_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG2458382.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            21335..22477
                     /locus_tag="KPK67_001390"
     CDS             21335..22477
                     /locus_tag="KPK67_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG2458383.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            22488..23282
                     /locus_tag="KPK67_001391"
     CDS             22488..23282
                     /locus_tag="KPK67_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG2458384.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            23293..24315
                     /locus_tag="KPK67_001392"
     CDS             23293..24315
                     /locus_tag="KPK67_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG2458385.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            24414..25790
                     /locus_tag="KPK67_001393"
     CDS             24414..25790
                     /locus_tag="KPK67_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG2458386.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(26122..26466)
                     /locus_tag="KPK67_001394"
     CDS             complement(26122..26466)
                     /locus_tag="KPK67_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458387.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
BASE COUNT        10111 a         3123 c         4941 g         8489 t
ORIGIN      
        1 aaaatcatag ctattttttt agtaattctt ttcttgtctg tatttatagg cagttttata
       61 tatatagata aaactttaag accgacaata acagttttag cagagactaa agctcttgaa
      121 ctagcaaata ggtcgataaa taaagctgtt gctgagatgg tagaaggaaa aataaattat
      181 gaagatttaa tggatataca acttgataat aatggtaaaa taactatgat acaagcaaac
      241 actataatga tgaatgaaat tgcatctgca atagctttag aaatacaaga tgaattaaaa
      301 aaggataaga cagcaagttc ttacatacct ataggaacag cattaggaag tcctatatta
      361 gctaaatacg gtcctaaact tgaagtatct attgaaccta taggaacagt atcagtaaac
      421 tttaaaacgg aatttgagtc ttcaggtata aatcaaacta gacatagaat atatttagaa
      481 gctcaaacac aagttaaagt agtaatacca cttataactt caacaaaaca aattaaagct
      541 caaataccta tttgtgaaac tataatagta ggtgatgttc cagaaagtta tgtaaatatt
      601 ccagaaaaaa atctaggaaa tgttttacct aacactggaa agaataccaa caagtaatat
      661 aaagttgtat atttttaaat aaattcaaaa taatagttta taaataaaat ttttggaaat
      721 aatattaata taattttaat aaaaatttat attttatact atgtaaaata tctaatatat
      781 ttggtctgta ggttaaataa aagtaccttt agaccaaata tattattaat aacttaataa
      841 catcttaaaa cttatttatt tatattattt acaacatcca tttaataaat acaccataac
      901 ctacagctgg gtcgttttca attgcatgtg acactgctcc gattatttta ttatcttgaa
      961 ctattggagt accactcata ccttgtacaa taccaccagt ttgagctaaa agctttttat
     1021 ctgtaatttt aattttaaaa cttttacttt ctggatgttt ttggttttct atatctataa
     1081 tttcaatatt atatttttta cacttattaa agttatcttg taaaattatt tgagcttttc
     1141 ctaatttaac ttcatctaaa gatgctactt tatatttttt taacttggaa atatcaaagt
     1201 tattaatttg acctcttatt ccaaaatcag tattttctgt aaagctacca ataatatttt
     1261 ctcgttttgc atttatacat ccaacactac ctttataaga tttttctata ttaaggtgct
     1321 tagttgtaga aattgaacct gttttaatag gtatttttct tgcattacca acacttattg
     1381 gatgacctac agctccaaac tctgatgtat ctggattgat atacgttaaa gtagcaaatc
     1441 cagaaagtaa attgttaaaa ctaatttttt ctaatatgtg tttggtagtt ttcattgtta
     1501 taagttgtcc acctctattt aataaaatag atacttgttt ttcttctggt aaagcattta
     1561 aggtattggc aaagtcacta tatccatcaa cttcagcatt atttaccttt actatagcat
     1621 cacctaatct aaatggagat gcttctccag gatttctaac tatgatattt tttaattctg
     1681 cgtcaatttg aagtacgttt ccaattggta ttaaatattc aggttcattt ttttgtaaat
     1741 tttgtgcata aattaaatta tttgagaaaa aatataataa aaataaaaat gttaaaacaa
     1801 ttgtaaattt tgacttagct aagttggttt ttttacttaa agtattattt ttaaaatgaa
     1861 aatttttaag ttgcataaaa cacctctttc ttattaaatt attttaataa tagaattgaa
     1921 gttttcttgc tgcttctata ttatattgtg caagaatcat gttttgatgc ttggaaaact
     1981 agctcaataa caccttagtt ttcataaaaa taaataaaat aaatagtgta tatgataaaa
     2041 taaatacata ttcatataaa taaaaataaa caataactgt ctagcataag aaaggagcaa
     2101 aaaatgaaaa aatatagatt gttagttact tgtataattg ccatgggaat cataattata
     2161 ggaacatcaa atccagttag caatttttac aagactacaa atttgattag ttcaggaaat
     2221 atcacaaatg aaaaagcaga taaagatgaa aattcaaaca aaaataataa agataaaaat
     2281 gaagacaaat ctaattctaa aagtaataaa gataagtcat cagaggacaa atcgtcagat
     2341 aataaaaatg aaattcaaaa aaataaaaaa cttttaattt gtatagaccc agggcatcaa
     2401 ggaaaaggag atagcaattt agaaccagta gctccaggtt cttcctctaa aaaagcgaga
     2461 gtatcttcag gaacagaggg tattgctaca aaaaagccag aatatgtttt aaacttagaa
     2521 gcatctcttg ttttaaagag tatattagaa tctaaaggtt ataatgtaat aatgactaga
     2581 gagacacatg atgtaaatat aagtaattca gaaagagcaa tacttgctaa tgacaaaaaa
     2641 gcagatatgg ttgtaagaat acatgcagat agtttaaata attcttcaaa aactggagca
     2701 tctatattga ttcctgaaaa agatggaaaa tatacagctc ctatatatga agaaagtaat
     2761 aaatgtgctg aatttatcaa acagaatatg gaacaatcag gtatacaaat caatggaatc
     2821 gttcaaagag gtgatttgac tggattcaac tggtcaaaag taccagctgt attagtagaa
     2881 atgggattta tgagtaacta taatgaagac caaatgatgt caaatccaga ctaccaaaga
     2941 aaaatgatgc agtgtatagc tgatggattg gatgcgtatt ttaaataaga tatgaataga
     3001 gaaaaagtta ggttggtatt attttcctaa tttttatctc tattttttat tgtacataac
     3061 acaaagaaga agtttatttt aggttaaatt gtcgtaaata taactaaaaa tattgggaat
     3121 acttgattta agtttaaaaa gaatatatta tagtatagaa tgtgtaaaat ttttttgaga
     3181 ggtgttgaaa tgctagtagt agaaaatgtg agtcatggat ttggtgcgag aacaatatta
     3241 gaaaatgtat cctttagact tagaaaaggt gaacatattg cactagttgg tgctaatgga
     3301 gaaggaaaat cgagtttttt aaatataata acaaagaagc ttatgccaga tgctggaaat
     3361 ataaaatggt cttcaagagc tacagtaggt tacttagacc aacatacggt attaagcaag
     3421 ggaaaaacta taagagaagt gcttagagag gcttttaagc atatgtttga cttagagcaa
     3481 gaaatgattg ctatgtatga caaaatggga gaagctagtg atgatgaaat gagtaagctt
     3541 cttgaagaaa cagcagagat acaaactata cttgaaaata gtggttttta tatgatagat
     3601 gcaaagatac aagaagttgc aaatggtctt ggtcttggag aaattggtct tgataaagat
     3661 gtaactgact taagtggagg gcaaagaaca aaagtacttc ttactaaact tctacttgaa
     3721 aatccaacta ttcttatatt ggatgagcct acaaactatt tagacgaaga gcatataaca
     3781 tggcttacta agtaccttca agagtatgag aatagttttg tcttagtttc tcatgatatt
     3841 gaatttataa ataatacatg taatgtaata tatcatatgg aaaatggaga gttaaataga
     3901 tataaaggaa attatgatga atttgtaaga cttaatgata taaagaaacg tcaagaagaa
     3961 caagcttatg ataaacaagt tgaagaaaga aaaagactag aggattttgt agctagaaat
     4021 aaagctagag tagcaactag aggaatggct aacagtagac aaaagcaact tgataagatg
     4081 gaaatattag aaagaccaaa agaaaaaata aaacctactt ttgcttttaa agatgctaga
     4141 gcagctagta aaataatttt tgaaacagaa aatttagttt taggatatga tgaagcattg
     4201 acaaaaccac ttaatttcca tttagaaaga ggaaaaaaga ttgccttaaa aggaatgaat
     4261 ggtattggaa aatctactct attaaaaact ttacttggaa taataaaacc atttgaagga
     4321 aatgttaaat taggtgacta tttggaagtt ggatattttg aacaagagag ttcaagagaa
     4381 aatagtaata cacctatgga tgaagtttgg agtgaatttc cagggcttac aaactttgaa
     4441 gttagacagg ctcttgcaaa atgtggactt acaaatgaac atataacgag tcaaatgaga
     4501 gtattaagtg gtggagaagc tgctaaggtt agactttgca aagtaatgtt aaaaaatata
     4561 aatttcttag tattagatga gcctacaaat catttagatg ttgaggcaaa agatgaattg
     4621 aagaaggcta tcaaagagtt taagggaaca gttcttttag tatgtcatga gccagaattt
     4681 tattcagaaa ttgttgatga tgtatggaat atagaagatt ttacaactaa aatcgtgtag
     4741 tgatttaact tattgctggg gggatttcat gaaaaagttt ctaaatgtac ttttttcaat
     4801 ttgtattagt attgttataa tagtaggtgt tattaatttt actgttggat ttaaacaact
     4861 ttattatttt gacattgatt acttaaatat atcagagtta tctggtttat ctaaagatga
     4921 tataaaattg aactacgatt atctaattga ttataactta aataagaatg tgtctgagtt
     4981 caaattacca acattaaaat cttctccaca agggaagata cattttgaag aagtcaggaa
     5041 tatatttcaa aatataaata aattagctaa attattattg gtagtttctt tagtaggaat
     5101 tatattgagt gtaaaaaata aaaatataaa aatcttgaaa actacatcaa taacacttat
     5161 aattatgcca ttattattaa cagttccaat tttattaaat tttgaaaagt catttattat
     5221 ttttcataaa ttattattta gaaatgatta ttggatattt aatcccgatt tagacccagt
     5281 tataaatata ttgccagagg aatttttttt tcactctgga atgatgattt taatattaat
     5341 tttgttagta agtatattac tgtttgtaat gtataaatta tgtaaatctt tatcaataaa
     5401 aatatttcaa tgttaaatag agggatattt ttatttccaa aataagataa taaaaaagga
     5461 ttagattaaa agttatacaa taaaaatagc tgtgcaataa tatttagact atgaaagtag
     5521 ttaaaaaata tttatgcaca gctatttata atagaattta ctttttacta gtgattttaa
     5581 ctaaatcttg aaatttactc ataatgtaat tgtaattatc ttttttatga ggtaatatac
     5641 aactactgca atctttgata cctttatctg tatacttaaa gtttccacca cagttttctc
     5701 ctaaagcata caaaggacaa tagcaaaata gacagttaaa ctcttctggt ttattagttt
     5761 tatgacaagg gaaaaattcg caatctttgt gattaaaaaa cttataattt tcgcccatat
     5821 tttcctccta attaaataaa atttaaattt tttatactat ataagatttt ggtgataata
     5881 taattatact aaataatttt atgaatagtc gataatatag tattattaat catagtatta
     5941 atggtataat tagtcaacag aaaaataatg taaaaataat gtaagtgtat tttgtaatta
     6001 gaatatgttt tataacaaaa aatgaggtga ttaagtgaaa gaaattcaag aaaaagcact
     6061 tcttgtaggt ctgaatttga ctacaatagt gaaaaaaaat gatgatatag acactaatga
     6121 atctatggaa gaattaaaag aactaacaaa agctgctggg gcagaagttg taggtagctt
     6181 aattcaaaat aaacactctg tagatgcagc ttattatata ggaaaaggta aagtagaaga
     6241 aataagagcc tatagtgact ctttagatgc tactttagta atttttaatg atgaattatc
     6301 tggtgctcaa atcagaaata tagagaatgt tgttggaaga aaagtaatag atagaactac
     6361 tttaatttta gatatatttg ctcaaagggc acttagcaaa gaaggtaaat tacaagttga
     6421 attagcacaa ttaaagtata gattgccacg actttatgga atgggtggtg agatgagtag
     6481 aacaggtgca gggattggta ctagaggtcc tggagaacaa aaactagaaa tagataagag
     6541 acatatactt aataaagcag cagatataag acgtgaactt aaagaagtaa aaaagaatag
     6601 agagacacag agagttaagc gtttaaagtc aaatataccg attgtagcac ttgttgggta
     6661 tacaaatgca ggaaaatcta ccctgttgaa cgaattaata aagacacaca aggattatga
     6721 acaagaaaaa gaagtttttg taaaagatat gctttttgca actttagatg taaccttgag
     6781 aaaggctctt ttgccgaata aaaaagaatt tttagtagta gatactgttg gatttgtaag
     6841 taaacttccc catgatttag tagaagcgtt taaggcaaca ttagaagaag tacagtatgc
     6901 agatttaatc ttacatgtaa ttgatgcaac aaatattagt tatgaattgc aaaaaagtac
     6961 gactgaaggt gtattaaaag aacttggtgt aaatgataaa aaacatatac ttgtatataa
     7021 taaagtggac aagctagaat tggatatata tcctaaaagt caagaagata ttgtgtatat
     7081 ttctgcaaaa caaggcataa atatggataa gttattaaat atgatagaaa ctgctttaat
     7141 ggaaaatact tattcagtaa gtttgatgtt accttatgag agaggtgata tatttagtag
     7201 aataaaagat aaatataatg tggagaattt tgagtatggg gaaagtggta taacgctaga
     7261 tgtagattta gatgaagaag attttaatat ctatagagag tacatactag aaaaataggt
     7321 tgaatatgga tataaactgg gaaaatatag ataatcttga agattatttt ataacctatc
     7381 ttctttataa agaatctaaa acagtatctc aaattagtaa aattagaaat atttcaagta
     7441 ctgaagcaag tgagcaactt atacaagcaa agttaaaaat aaaggaaatg caaaaagatg
     7501 attttgaggc atctaaggat attttggata aatttctaga actagataaa attaagagac
     7561 ttgattttat ggatagctta gatgacgaaa aaatggtata ctttaagaga aaggttttca
     7621 aaagaatcct tgtagaaaaa aatgctgaag atttaattgt tttaatttgg gcgacaggtg
     7681 agctaaaaga tgataggttt ttgaaattgc ttcatcagct cacaaaccac agacactcag
     7741 atattagaag aataacttat tcagcaatca gaaagattga atcaccaagt agtagagaag
     7801 tactgcaaaa aggtctctac gacaaaaatg cacagactag gcagtactgt gcaaaagctc
     7861 tttcaaaatt gggagatgaa aacagtttaa agattttaca gcagttgaaa gacaagaata
     7921 agaattttga aaaagaatat gtgcttagag cttatgacga agccattaag tcattggaaa
     7981 atgcaaaaaa tttgaaaaag taatttttta ttaaagcaat ttgtttactt aatgatgtat
     8041 ttctaaagtt taacaaggta ataaataaat taatgggagg ggtttcaaat gatagtaaga
     8101 cgttgtgcag gtggagtagt gttttatgct aataaagtac ttattgtaaa aaatgatagg
     8161 ggtgaatgga cacttccaaa aggaaagata ctaggagggg gacttccata tgagagtgct
     8221 gttcaaagag ttaaagtaga aactggcata gatgctaaaa tgatagatgt ggctggcgat
     8281 actatgtatg aatttttctc aagaagtaga caacaagaag tttgtaatgc aataatgtgg
     8341 tatgttatgg aagcttgtaa cactgattgt gtacttgctc ctgaatttca agaaggtgga
     8401 ttttataaag taaaggatgc tttagaaatg ttatcacatc ataaagagca agctttagta
     8461 gaagtatctt ataaaaagtt taaggaatta aagaaattag tttcagaggg cgatgatgtt
     8521 ccagcaaatt aattgtgaat ttagaggatt ctgaggaagg gatagcccct tcctttttta
     8581 attgaagcag ttttaagcgt aaattatata gtctctttaa actatgattt atcaatttag
     8641 agatttagta gtttatattt aaaatcagta agatatattt aaaataaaaa ttaaaaagta
     8701 tacaatttta ttatataatt gtcttatgat gttgattaaa gaagtattaa aagtccattt
     8761 atttttaaat agaataattt tataaatata tatttgatgt gtatattaga aaatgattaa
     8821 aataggagga aatacaatga gcaattacag gacattacat gaatttggaa cggatgaaat
     8881 aacgattgaa aagtctgttt ttattggata tgctaagcct ataaagagtg aagaagaagc
     8941 attagagttt ataaatgaaa taaagaaaaa gcataaagat gcaactcaca atgtgtgggc
     9001 ttatactgta ggtaaaaata tgaatataca aagatatagt gatgatggag aaccacaagg
     9061 aacagctggt atacctacat tagaggttat aaaaaaagaa gatttgagag atgtagcggt
     9121 tgttgttact aggtattttg gaggaacaaa attaggtgca ggtggtctag ttagagctta
     9181 tacaaaaggt gctaaactgg gacttgaagc aggtaagata atttataagg taatgtatca
     9241 agaagttaag gttaagatag attatactca acttggaaaa gtgcaaaatg agttaatgaa
     9301 tttggggtat tttataaaag atactgtata tgaggataat gttgaaatag tagtatattc
     9361 aagattagaa gatgtagaga aattatcaga aaaaatgatt gatataacaa gtggaacagg
     9421 aaaaatagtt ttaggagagg aattttattt gtctgaacaa aatggagaaa ttttaatata
     9481 atctaaaagg ttattagaat agttaatacc tcaaatagcc aattagtatt tttaagtgaa
     9541 aagccattaa attgatttaa tctaaatcaa tttaatggct ttttatcaga ttttgcacaa
     9601 aatccttttt ttacttcaaa taaaccgact aaaagtaaaa atatcccaaa tatttttttt
     9661 aatatatctg aagacagata attagctaat agtgaaccaa atacagctcc taaaacccca
     9721 aacacagcta taggtataac caatttaaaa attacatttt tattttttat atgtatagca
     9781 agtgctataa gtgtcatagg gatagatgaa agtaaattta cgctttgtgc tattttagtg
     9841 tctatgccag caagtaaaac gagtgcaggt ataaggattg ttcctccacc cattcccatt
     9901 cctccaataa ttcctgcaaa aaatccaatt agagcaaata acattaaaac accatcctta
     9961 atgctgcaat tatcataatt attccaaaag aaattctaag aaactttcca gtaaccttat
    10021 ttagtaattt agctcctata ataccaccaa gaatacttcc aactgcaact ttaattgtta
    10081 agttaacatc ataagtacct ttagatacat ataaaacaga acttgctgtc gttaagaata
    10141 ttattataga aagagcagtt gcatgagatt tatgttcttc taccttaacg atgtcattta
    10201 atattggaac taaaagagta cctcctccag aaccaaaaac tccatttata aatcctgtaa
    10261 aaactccaat tatcgtattt tttaagttaa aatcaaagta ttttttcttt aaatcatcag
    10321 tttttttcat aaaaatcacc ttctgaaata gttttaccaa aataggcaaa ttatatttat
    10381 ataaaataag attaaatata atctttcatg tttgtttaat tttgcataaa aaaaaacata
    10441 ttaaaacaac atattaatat atggtataat atattaaaac aaagtagcaa atttgagagg
    10501 agataacaat gtcaaatata aaaatacaaa tagacaaaac tgtagaatgg cttataaata
    10561 aggttaatga ggcaaatgca aaaggattaa ttgttggtgt atctggagga attgattcgg
    10621 cagtagttgc taatttaatt aaaaaggcat ttccagaaaa ctctatggga gttataatga
    10681 gcataaaaag taatcctcaa gatagagaag atgcacttaa ggttatagag ggatgtgata
    10741 tagaatacct tgatttagac cttatagaac cacaaagtgc tatattagac atggtagtag
    10801 gaaatttaaa agataaacat ttatatagag aagaatattt aaagatgaca gatgcaaact
    10861 taagagcaag agtaagaatg agtacaattt atacaattgc taacaatcta ggatatctgg
    10921 ttgttggtac agacaatgct gcagaaatac acacaggata ttttactaaa tttggtgatg
    10981 gtggagttga tattttacct attgcaaatt taactaaagg agaggtgtat gaatgggcaa
    11041 aagagcttgg tgttcatgaa gatttaataa acaaagctcc ttcagcaggt ctttgggaag
    11101 gtcaaacaga tgaagatgaa atgggaacta cttacaatat gatagatgct gtgttagaag
    11161 gtagattgga tgaagttcct aaaagagacc aagagataat tgaaagactt catagattaa
    11221 gtgaacacaa gagaaaaacg cctgcacaac ctcctaagtt ctaatttact atttataaga
    11281 tttgtgataa aatttacaag tgatgaaaat tagaataatt aattgtacgt attaaggagt
    11341 ggaaatatgg gacgtatagg taatataatt aatagaaaag gtaaacaaga tgctcaaaga
    11401 gcaaagatat ttactaaaca tgctagagca atagcagttg cagctaaaga aggtggagca
    11461 gacccagagt acaatgcagc gttaaaaaca gctatagaaa aagctaaggc agataatatg
    11521 ccaaatgata atatagatag agctatcgct aaaggtgctg gagcaggtgc tggagaagac
    11581 tatgaaacta tagtttatga aggatatgga cctggtggag ttgctgttat agtagaaact
    11641 ttaactgaca acaaaaacag aactgctggt aacgtaagat attattttga caaaaacgga
    11701 ggaaacttag gaactagtgg atgtgtgtct tttatgtttg ataaaaaagg acaaatatta
    11761 gttggtttag gagatggagt ttcagaagaa gaattgatgg atgttgcatt agaagcagga
    11821 gctgaagatt tcataacaga agaagatggt tatgaaataa taactacacc agaagatttt
    11881 tctagtgttc gtgatgagtt aaaagctaaa ggatatgagt ttatatctgc tgatgtgaag
    11941 atgatacctc aaactacaac tgtattgact gaagaatctc acttaaaaat gatgaataaa
    12001 ttagttgata tgcttgaaga agatgatgat gttcaagata tatatcataa ctgggaagta
    12061 gaataaatag tataagcata ttgaaattta taagtattta atcaatttta acatgtgata
    12121 aattttatag aataagaaac actaaaatag acatacgcac ctctaaccta tatttgcata
    12181 ataatatgta agcataggta aggggtgttt ttatatttat aggttaagaa atttaaagta
    12241 aattttgagc tggactcgat tgtctataaa taattgatgg gtatataatt tgttgctgaa
    12301 taccatttat acgcccagtt actgtatcaa taatcatcct aagactacgt tctgctgtct
    12361 cttccatttt taaatctata actgtcaaag gaggaatact tcttgccatt agtcgtgaag
    12421 gtccattact tgctgcaaat atttgaatat cctctccaat tacaattccc gattcatgaa
    12481 atgcattcat cataccaaga gctaccacat catgattaca aaaaattact ttttttagtg
    12541 aattttttgc aagcattttt tgagcaatat catatccact atctatttta ttagaacaat
    12601 aatatataga atctgataga tgtattccat gttctttaca tgtttttaaa aaagatttac
    12661 cccttaaagt catacatgta aaggaagttg ggttgaatac aacagcaata tcatttttac
    12721 cattttgaat agcatgtaaa gctgctatac gaccagtttc ctcattatca atagatacac
    12781 ttgaaaaacc aggtatttca cgatttacaa caactacagg aattttaggt ttatttttta
    12841 ctagatattc tatatcatca gcattgatac ctgttaaaac aattccatca taataattat
    12901 ttgcaaaaaa gtattcatca tcacatagat gaccaacttt gtaaggtcgt atgatgatat
    12961 ttatgtttaa atgttctaaa gaaattatgt ggtttattgc ttgaagtata ttggttgatg
    13021 ccaactcaaa tccctcatcc tgccaataaa aagctatttt aatattatta tgtttaatag
    13081 gagtagagcg tttatatcca agcttatcag ctgaatttaa tataagctct tgtgtatgta
    13141 gcggaatcct gcgttcattt cctttaccac ttaagacgta cgagacagtt gctgaagaaa
    13201 gaccacagtc ttttgcaata tctttgattg ttgccatgat aaaatcctcc ttaaatagtt
    13261 gcttaaattt gcttatacca ataatcttaa catatattca actaaagaac aatattctac
    13321 aaggtgcata ttttacagga ttagaataaa taaattaaaa aaaacttctt acttatgttg
    13381 acataattta gtttatagga ttataatatg cttaaataag cttataatta acttaaatta
    13441 agcaaagtta agcaggaaca tgcaatgcta gcttgcaatg tgactgtaaa gggggaaaag
    13501 gtatgagtaa ggtaaatata tttgaagttg gtcctagaga tggatttcaa aacctgaaag
    13561 agtttttaga aactgataaa aagcttaaga ttattgatgg tcttattgct tcagggatta
    13621 aacaacttga aattagttca tttgtaagcc caaaagcaat acctcagatg aaagatgcaa
    13681 aagaagtagc aacttattgt gtagaaaaat atcctgatgt gaaactttat gcattagttc
    13741 caaatttata cggtgctaaa gttgcatggg agtgtggtat aagagatata tcctatgtta
    13801 tatctttgag tgaaactcat aataaaaaaa atataaatcg tacccatgaa gagtctattg
    13861 atgagcttaa aaaaataatg aatagttatc cagacatgaa tatatgtatt ggaatggcta
    13921 ctgtatttgg atgtcctttt gaaggtttac caaaaattga agatgccata agatttgcta
    13981 aacgcctttg ggaaaacggt atcagagaga taaatctagc agatacaata gggattgctg
    14041 accctaagca agtaagagaa actattagtg cattagttga agctttacca gagtgcagat
    14101 ggcaagtaca tattcatgat actagaaata tgggaatggt taatactctt gtggctattg
    14161 aatctggaat tactactgtt cagtctacac ttggaggatt aggaggatgc ccatttgctc
    14221 ctggagcatc tggcaatact gcaactgaag atttagtata tatgcttgaa cgtatggggt
    14281 atgatacagg agttgatttt gttaaacttc ttgaattatc aaagtatcag aagagttttg
    14341 taaatggagt atatagtggt catcatgtaa atataaagtc tgatagttgt aattgttagt
    14401 tctattgatt aatcgattaa aaaaatatgt tgattaaaaa aatatgctga ttggtaaaat
    14461 agatttattg ataaaaaata taaattataa aatacattat ctaataaaac aaataaaaaa
    14521 attgaggggg attagtatgg atattatagc tttactttta tttttaggag ttattattat
    14581 tgcatttgta cgtaagaata atgcaggtat tttggctcta gctattggtg tcatagctgt
    14641 tagaattttt ggaatggatg ataaagaact ttatggagct ataagcaact caatgtttac
    14701 tacacttgtt ggtataacac tactctttgc aatagtgaac tcaacaggag cattaaagct
    14761 tgttgcagaa aaaattgtag ctgcttctgg taaacgtgtt tggcttatac ctatagcagt
    14821 ttttatagca ggatttgttg ttgctggtgt tggacctggg gcaattccag catttgcaat
    14881 tatcccagca atggctgttc ctgtagccat aacggttggt tataatcctg taatgcttgc
    14941 acttataggt gaatgtggat taatggcagg tcgtatgact cctattactc cagaaggtca
    15001 aattataaca accgttgcag aaggggttgg aattgaaaat gttatgccta ctatcttagc
    15061 ctgtcaaact ctttctacaa ttgtattttc aatagtattg tttataattt tcaaaggata
    15121 taaacttaaa aaacctataa atgttataga tagtaagcaa atagaaaaat tcaatataaa
    15181 gcagataatt tctctaataa gcatagtagt tatgattgct tttatcataa tttttgatat
    15241 gaatgttggg cttgcagctt ttgcagtttc agctgtactt atttttttca atattgctga
    15301 tgaaggagaa tgtatcaaat caattccatg gagcacgata gtaatggttc ttggagtagg
    15361 tgcaatgatg acaatcgtag atgcagcagg aggtattgac ttgatgagta atgcattatc
    15421 atcactgatg aatagtaaaa cagctactcc aattatgagt atttcagcag gacttatgtc
    15481 tatggttagt tctgctttag cagtagttta tccaactatg atgcctatgt gtgttgatat
    15541 agctaaggca gttggaggag tagacccact agcacttatt gctgcagttg gtgttggagg
    15601 ttctcttgct ggtgtaagcc cactttctac tggtggggca ttgattttag cagctatggg
    15661 ttcaagtaag aaagacttta ataaggaaga acaaaataag gtttttgtac agcttttcat
    15721 tatgagtgca gttggcttat tggttattgc agtagtatca atattatcat ttaatgcaat
    15781 aacacattta atgaactaat tagaataact gaaaataaat cattaaattt aacttttaat
    15841 attaatcaat ataatataat ttggagggga agttatgaaa aaaggagcat taagcaatat
    15901 aaaggtactt gaccttacaa gagttcttgc aggtccatat tgtacaatga tgttggcgga
    15961 ttttggagct gatgttataa aaatagagat gccaagaaaa ggtgatgata caagaggttt
    16021 tggacctttt gtaaatggag agagtttata ctatgcaaat ataaatagaa ataaaaaagg
    16081 tatcactcta aaccttaaat cagaagaagg aaaaaatata tttttagaaa tggtaaaaca
    16141 tgctgatgtt gtagtagaaa attacagacc aggtgtaatg gaaaagttag gtctgggtta
    16201 tgatgagttg gctaaagtta acaatcaaat aatttatgga gctgtatcag gatttggatg
    16261 ttatggacca tattcagaac gtccaggtta tgatattatt gcacaggcaa tgggtggtct
    16321 tatgagtata acagggcaag aaggaggaga accaacgaga gttggtaatg ctatgggtga
    16381 tgtactaggt ggaatgaatt taactattgg tatactcaca gcactcaacg ctagaaaaat
    16441 aataggaaaa ggacaaagag tagatgtttc cctcgttgat tctgttgtat cgtctctcga
    16501 aactggtata cagagatact ttgattcagg acaacctgaa cttatgggta atagatatgc
    16561 ttcagcctat ccttatgact cttttaaggc aagtgatgga agttttgtaa taggctgtgg
    16621 taatgacaaa ctttttgaaa agttatgtaa aaaagttctt tttagagaag accttttaat
    16681 agatgagaga tttaaaacaa atcttaatcg ttgtgaacat tacaaggcta ttaaaccaga
    16741 aatagaaaaa tggtctgtta agcatacaat aaaagaagca gttgacttaa tatcagaggc
    16801 tggagttcct gctgctccta tctataatct tgattgtgtt acaacagatg aacatatagc
    16861 aaaagcaaga gaaatgattg tagatattag tcatcctatt ataggaaata tgaaagtcaa
    16921 tggaaatcca gttaagttga tgacaacaaa agctaaaatt gatatgccag caccatctct
    16981 aggggaaagt aacgaggaga tttataatgg ttggcttggt tttgatagtg ataaaataaa
    17041 agatttaaaa gaaaatggag ttatttgatt aaaaaatagc taaacacatt tgtgacttta
    17101 gtcatgggag gttcagttag aataatatca aaaaatatta aatcaatact gtaaataaaa
    17161 tatagaattg tcagaagcat ttaagatggg aaaagctact tagacattga gtctttgtgg
    17221 cttttcctat tttcattaag aattttatat tatcagaata gatatgtact aaactaaagt
    17281 attaacaatt taaattaata gaaaataaac tacctaatta tgttgaataa agactacttt
    17341 agtgaaaata aacttataga gatgctattt aaattatttt aatggaaata tcaaaaatga
    17401 gactgcattc tcaaaaatga gactgcgttt tttaagtatt ttctcgaaaa tgagacaaaa
    17461 atattgtttt atgggtttta ttgaagaaaa attcttctgt gaattacaat ttaatcaatt
    17521 ttaaagttga aaattattaa aaatttagtg aaatttatgt atatttcgtt tattttatta
    17581 aaaataacaa ttttttacta aaaataaaga acttcaatgg tgattggcat agtacttgct
    17641 attttatatt aacaagaaat gtagaaaaaa taacaatgta taatgggggg aatatgatgg
    17701 aatctgtaat ggaaaatttg aacaatctta aggtggaggt taaggacaaa gtctgtgtta
    17761 ttactataaa tagacctaaa gctttaaatg ctcttaatag tgacactctt agggaactat
    17821 cacaagttat tgatgtagta tcagaaaatg aggcaatatt aggagttatt attactggag
    17881 aaggtaaagt ttttgtagcc ggtgctgata tacgccagat gcaaaactat aaaagtgagg
    17941 aaggcagaaa atatgcaggt tatgctcagg gaatttttga taaaattgaa gctcttgaga
    18001 aaacagtaat agcagcagtt aatggatatg cattaggtgg tggatgtgaa cttgctatga
    18061 gttgtgatat tagaattgca tcagaaaagg ctatatttgg acaacctgaa gttaatttag
    18121 gagttattcc atgttttggt ggaactcaaa gattatcaag acttgttgga actggaatag
    18181 ctaaagagtt aatttttacg gggagacaag taaatgcaga agaagctaaa tctattgggc
    18241 ttattaataa agtggttcca agtgatttac ttttagaaga gtctatgaag atgatgaacc
    18301 aaatagttga aaaagcgcca atagcaataa gatatgcaaa agttgttatt aacaaaggga
    18361 tagatatgga tttaaagaat gctcttgaac tagagaaaga tattgcaggt ttaacttttg
    18421 ctacaagaga taagcaagaa ggaatgaatg catttataga aaagagaaag ccagtttttg
    18481 aaaataagta gctattataa ataaagttgg aacaatgtag aatatattca aaatctaaat
    18541 ttcgtgaaaa aatacatata gtttaatttt aaaaggagtt aatttatgaa tacgggagta
    18601 attataagtt tagtagggat tatcatagct ctatctcttc tagttgtaat ggttatgaag
    18661 ggaataaata tttttattat agcaattgta tgttcatcat tagttgctat aacaggaagt
    18721 ctaaatgtgt atgatgcact taaagtggat tatatgacag ggtttgttgg atttcttcag
    18781 gcaaatttct ttattttcct aacagggaca ttaatgggaa aaataatgga aattactggt
    18841 ggagcaaaat ctattgctaa gatgattgta agatggattg ggaaggataa agcactactt
    18901 tctattccaa tagcttgtgg aatattagcc tatggtggag taagtgtttt cgttgtaagt
    18961 tttgcagtgt ttccgatagc tttagaaatt tttaaagaag ctgatttgcc acgaagattt
    19021 ataccagcag ctttgacttt tggatgctca acttttgcta tggttgcacc aggggcacca
    19081 caaattcaga atattgttcc agcatcaaca ttagggacag atattatggc tggagcagta
    19141 aatggtttta tatcatgtgc agtaatgttt atagttggaa gtacaatttt gtacaaaatg
    19201 gtttcaaaag aaaaagccaa tggtggtcat tttatagcac atgaatcaga tatatttgat
    19261 gaagcagaag caactaattc taagagagaa aatggtccca atggtgtaat tgccttaatt
    19321 ccattgatag tgtcaatttt aataattaat gtaaaagtta atggaaaagc gatagtacca
    19381 attgaggtgg gagtatttat tggagcttta ttagtatatt tattgttaaa taaatatcaa
    19441 gataatacta aaatagttgg acatgttggt gatgcatgta aaactacagt agtagcaata
    19501 tgtaatactt gtgctgtagt tgcatttggt tcagtagtaa aatcagcagt tggttttgac
    19561 ttcatagtta atgccatgac taatattcct ggtcctccta ttgcaggggc tgcagttgga
    19621 actacagtta ttgcaggtat ttgtgggtca gcttcaggtg gactaggaat tgcagctcca
    19681 cttcttggac cagtattttt agccaagggt gtttccgctg cagcattaac taggactatg
    19741 gcaatttcat cagcagcatt agattcactt ccacacaatg gatatattgt tactgtaaca
    19801 aatggtcttt gtaaagaaac acataaggat gcctatatgc caatattctg ggtaactgtt
    19861 ttaactccgt ttattggaac catagtagca gtcctactgt ttacaatgtt cccgggattg
    19921 ccataagaga aaagataaaa ggtataaatt atattttgat attataaaat caattttcaa
    19981 aatatataca ataaatttca tgttttaata ctgtaaatat gtatttaaaa taacaaattg
    20041 accatataag gaggaattga aagtgttaaa taaaccatta gagggagtta aggttattga
    20101 cctaacgtat tttgtagcag gtccagggac ttctaaaata ttagctgact ggggtgctga
    20161 cgttattaag gtagagccaa gctttggaga cccaggaaga aagacagggg caacaatgac
    20221 aatgccaata gatgattata ataatccttt ttacagcaca tataattcaa ataaaagagg
    20281 tttgtctata aatcttaaga gtgaaactgg aattgaaatt atggataagc ttttaagcga
    20341 agcaaacgta tttgtttcaa gttatagaac aggagcatta aaaagactag gtcttgatta
    20401 tgaatcatta agtaaaaagc atccacacct tatctggggt cagataaatg gttttggcga
    20461 ttttggacct gctaaggata atgcaggatt tgatacagtt gccttttggg caagaagtgg
    20521 ggctatgctt gatatagcag aaaaagatac aagtccaata aatccaccta tagcctttgg
    20581 agatgcaaca acttcttgta gtttatctgg aggaatatgt gcagctttgt accagcaagc
    20641 taagactgga aaaggacaaa aagtaatggc atctttattt gggcaagcaa tatggaatgc
    20701 atcatcttta ttagcatcta cacagtttag tgatgagtat ccaaaaacta gaaaaaatgc
    20761 tatttcacca gtaataaatt cttatcgttg taaggatgga aagtggatat tcttaagtat
    20821 tttagagcat gaaagatatt ttgaggcact ttgtagatta tttggaagag aagacttaat
    20881 aaaagaagag aaatttgcat cttcagtaga aagtaagaaa aatgctacag aacttattaa
    20941 tattttggat ggtgaatttg caaagtttac tcaaaatgag atggttgaaa aacttactgc
    21001 tgctgatata gctcatgaaa aaattcaaca tgttaatgat gtagtgactg atgaacaggc
    21061 tattgctaat aactatatat atgaacatac aagtaaaaat ggaaataaga caatgcttgc
    21121 atctactcca gttaaatttg gaaatattga agttaacatg acttgtgatg ctccacttat
    21181 aggagaacat tcagatgaga tattgagaga acttggatac agtgattctg atatagaggc
    21241 tctaattgaa tctggaattg taacaatgaa taaagaagca tgtgaaatat gttaataaac
    21301 atgtgaagta tgttaataaa taggagggaa ttttatggat tttagattaa cagaagcaca
    21361 attaatgctt caaagagtag caaaagaatt tgcagagaat gaaatagcac caatagctgc
    21421 tgaaacagac aaaacaggta tttttccaag agagcttttt agcaaaatgg ctaaaatagg
    21481 atttaatggc attggaacac ctgtagagta tagtggttct ggtggggcag atatagaaaa
    21541 ggtaattgtt gttactgaga ttgccaaaaa atgtgcagca tctgcagcaa tattatcaat
    21601 acacacaata tatgctcaag ctattttgaa atttggtact gaggaacaaa agaaaaaata
    21661 ccttccaatg atggctgaag gtgggtgtgt aggtgctttt gccttaaccg aacctaatgc
    21721 tggttcagat gctgcaagag ctgcaacaac tgcaattatt gatgaagaaa ctgatgaata
    21781 tgttttaaat ggaactaagt gttttatttc tggtggaggt caagcagaat cattaataat
    21841 atttgcactg acagacccat caaagggaat aaagggaatg tctgctatca ttgtcgacaa
    21901 aggtactcca ggtttttcaa taggaaaaat tgaagaaaaa atgggtattc atggttcaga
    21961 aactgcagag ttaatatttg ataactgtag agttccaaag tctaacttat taggaaaaga
    22021 aggcaaagga tttaatatag ctatgacttg tttagatgga gcaagaattg gagttggagc
    22081 tcaagctgta ggaattgctg aaggggcttt agaagaaagt atcaaatatt ctaaagaaag
    22141 agtccagttt ggcaagccaa tttcagcttt gcaaggtatt caatggtata tagcagatat
    22201 ggcgacaatg gtggaatcag caaaattatt agtatattat gctgcagatt tgaaagctag
    22261 aggagaaaaa cacactaaag aagctgctat ggcaaaatac aatgcgtcac gtactgcaag
    22321 agaggttaca aatttagccc ttcaaatcca tggtggatat ggatatatga aggattatcc
    22381 attagagaga atgtacagag atgccaaaat aacagaaatt tacgaaggta cttcagaaat
    22441 acacaaagtt gttatttcaa gagcagtact aggataggag gaaaagaatg aatatagttg
    22501 tttgcttaaa gcaagttcca gatactaatg aagttaaaat aaataaagag acaggtactc
    22561 taataagaga tggtgtacca agtataatca atccagatga tagaaatgct ttagaagaag
    22621 ctctaaaaat gaaagatgaa ctaggagcag tgattaaggt tataagcatg ggacctcctc
    22681 aagctaaaag tgctttaaaa gaagctttgg caatgggagc tgatgaagct tatttaatct
    22741 cagatagagc ttttggtggt tcagatactt gggctacatc tacaattatt gctgctgcta
    22801 tagagaaggt tggaaagtat gatgtaatat tctgcggaag acaagctata gatggagata
    22861 cagctcaggt tggaccagag gttgcagaat ttttagggat accacaagtt acttatgcga
    22921 aagaagtaaa ggttcaagat gacaaattac tagtaactcg ttatacagag acaggggatt
    22981 atttaataga agcaaagatg ccagttttat taactgctat aaaagagtta aacaatccaa
    23041 gatatccaag tgtcaaagga atcttagaag cttataataa tggagatgca aagattactg
    23101 tattaacact tgcagattta gatgttgata caactcaaat tggattaaag ggttcaccta
    23161 caaacgttta taagtctttt gtaccagtta aagacaagca taatgaaata atagaaggta
    23221 taaataaaaa agaaaaagct gagaaactaa tagaaatact attcgatttg aaactagtat
    23281 aggagtgtta agatgatgag agcaaaagtt aatcaaggta taaatttaaa tgattataat
    23341 ggtgtttggg taataggaga gcaaagggaa ggtaaaatta atcctgttac gatagaactt
    23401 attggtgaag gaagaaagct tgctgaccaa ttaggaaaag agcttgcagt tgtgatagct
    23461 ggatatgaag tagagaaaga agtgaaggaa ttacttcact atagtgttga taaagtatat
    23521 tacatcaatg accctcttct taaagatttc actactgatg gatatgcaat ttcaattgca
    23581 aatttaatcg aaagaaaaaa gccagaggtt gtattggttg gtgctacttc tataggaaga
    23641 gatatagcgc ctagaattgc aggtaaggtt ggaacaggtc taactgctga ctgtacaaaa
    23701 cttgaaatag attcaacaga taataagtta ttacaaacaa gaccagcatt tggtggaaat
    23761 ttaatggcca ctattgtttg tccaaaaaat agaccacaga tgtcaacagt tcgtccagga
    23821 gttatggcaa aagctgtaag aaacgaatca gagactggaa ttttagaagt tgttactcca
    23881 gaacttactg aaaaaatgat tagaactaga ttagtagaga tacttccaca agaaaagaag
    23941 tctgtaaatc taactgatgc tagaataata gtgtcaggag gaagaggatt aaaaagagca
    24001 gaaggatttg agcttataaa agaattggct gataaattgg gggcagagat tggtgcttca
    24061 agagcagcag tagattcagg gtggatagaa cattctcatc aagtgggaca aacaggaact
    24121 actgttagac cagaactata cattgcatgt ggaatatctg gagcaataca acatttagca
    24181 ggtatgagtg attctaaata tatagttgct ataaataaag atgcaaaagc ccctattttt
    24241 agtatatgtg actatggaat agtaggagat ttatatgaaa ttatacctga aatgatagaa
    24301 tcattaaata ggtaactttt agaaaacatt gaatatgtag aatgataagt cttataatat
    24361 tgtattgtaa gacttattat ttttgctgaa ggttatagtg gggagatgaa acaatgaaag
    24421 agcagtggta taaggatata tttgcaagag tattatctat gactgatgat ggttttattg
    24481 ttgtaaatac aagtggagta ataatagata ttaatgataa atattgtgac tttttaggaa
    24541 aagaaagaaa agatataata ggacaaaata tacaaagtat tataccaaat acaaaaatgc
    24601 tagatgttat gaaaaataag tattgtgaag aaggagctat acatcattat tcaggtggaa
    24661 atacaaaaga aaaaagtgta atagtaagta ggtcttatgt ggaaaatgac aatggagaag
    24721 tagttgcagg agtagctcaa gttaaattta gattgcaatc atttgatgta gctaaaaagc
    24781 taatgtcaga atatatggag ctccaatatt ataaagagca gtttaaggat aactgtggtt
    24841 ttgacaagct cataggtgaa aatagagact tcatagaact taagaaaaca ggtgtgaaag
    24901 cttctaaaac aaactttcca gtacttttaa cgggtgaaac aggtacagga aaagaagtct
    24961 ttgctagagc aattcacaat aatagcagta ggtcagataa acctatggtt agtataaact
    25021 gcgctgcaat accagaagaa ttattagagt cagagctatt tggctatgac gaaggagctt
    25081 ttacaggagc aaaaaagggt ggtaaaaaag gtaaattttt agttgcgaat aatggtacaa
    25141 tctttttaga cgagataggt gacatgcctt tgaccatgca agcaaaactt ttaagagttc
    25201 ttcaggaaag tgagatagaa ccagtgggag gtcttaaaac tataaagata gatgtaagag
    25261 taatctctgc cacaagaaag aatttaagta aaatggttga agagggatta tttagagaag
    25321 acctatacta tagattaaat gtaataaaca ttcatatgat ggaattgaag gacaggcaag
    25381 atgatatttt attgctagca aattatatat taaacaaatt aaatgttgaa tacaaggaat
    25441 tgaaggtttt aagtgataag gttaaaaatt gttttattaa ctatacttgg ccaggaaata
    25501 taagagaact acaaaatgta ataaaaagtg cctatgctgt aagtgatgat atggtcatta
    25561 tgatgtgtga ccttccatct aagatggata atatcagcag agtagctcaa tgtaatgtgg
    25621 atagcaattg ttcaattcat gaaatggttg agaattatga aaaaagctta attattgatg
    25681 tgttaaggaa gtataattgg aaatgttcta aggcagcaga ggttatggga atacacaaga
    25741 gtcttttata taagaaaata aaaaagtatg aaattgaatt aaataattaa gataacttga
    25801 attaaggcaa tgctaaaaaa acgcccttat tcaaagcgaa ctgtataaac tgttttatgc
    25861 agttaaaacg tgtaaaagta gatgtgaagt actagtgtca atcattagta cttttttatt
    25921 gtatttataa ttactatcta tatttcagta tcttttgata tctaagatta ttatgtttaa
    25981 tttaggatat aacaaaaccc tcttagttat ggacagtttt ttatatttta actgtctact
    26041 aagaggggat agtttagttt aaatttagag taaattttat ctagcattta taaaaaatat
    26101 tttgaatagc ttttttgtta tttagtcata attttctact tctttatcaa gttctttgcc
    26161 acaagaatca cacttatata tagcattgta tccgtaatca tattcatcaa ctattttctt
    26221 ttgtctaaat acaccaccac actcacatag gaaaccaatt tttataagtg taacgataaa
    26281 ttgtgatttt ataaatttta tatcattaca tttatcgtta gtgtaaaatg tataaatata
    26341 agagttatcg cactcatctc ttaattcaaa tctttcaata tatgtgttat tttttaattc
    26401 ctcaaattct tctttagtta aatatgattt ttgttgcaat tcatgtaaac gcttaatttg
    26461 gtccattatt tatatcctcc caataatttt agtattttaa gcttagataa ggaatctaag
    26521 ttttattaaa tttatcccaa ggaattattt aaatcatatt tagttttatt aattacaaca
    26581 tatataaatt acacgaaaat aaaactaatt gtaaaaataa tatcctgtga aaacctaaac
    26641 tactaacccc ttactaaccc gaaa
//
LOCUS       DAEMHW010000039        26219 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEMHW010000039 DAEMHW010000000
VERSION     DAEMHW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26219
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.039"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(338..1117)
                     /gene="hisJ"
                     /locus_tag="KPK67_001395"
     CDS             complement(338..1117)
                     /gene="hisJ"
                     /locus_tag="KPK67_001395"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBG2458388.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGAEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFSYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKESYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(1663..2013)
                     /locus_tag="KPK67_001396"
     CDS             complement(1663..2013)
                     /locus_tag="KPK67_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBG2458389.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(2029..2445)
                     /locus_tag="KPK67_001397"
     CDS             complement(2029..2445)
                     /locus_tag="KPK67_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBG2458390.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(2464..2655)
                     /locus_tag="KPK67_001398"
     CDS             complement(2464..2655)
                     /locus_tag="KPK67_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBG2458391.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(2806..3621)
                     /locus_tag="KPK67_001399"
     CDS             complement(2806..3621)
                     /locus_tag="KPK67_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBG2458392.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(3668..4606)
                     /locus_tag="KPK67_001400"
     CDS             complement(3668..4606)
                     /locus_tag="KPK67_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2458393.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(4603..5475)
                     /locus_tag="KPK67_001401"
     CDS             complement(4603..5475)
                     /locus_tag="KPK67_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2458394.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYVEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(5500..7428)
                     /locus_tag="KPK67_001402"
     CDS             complement(5500..7428)
                     /locus_tag="KPK67_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBG2458395.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(7540..7917)
                     /gene="gcvH"
                     /locus_tag="KPK67_001403"
     CDS             complement(7540..7917)
                     /gene="gcvH"
                     /locus_tag="KPK67_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBG2458396.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(8004..10130)
                     /gene="cdhC"
                     /locus_tag="KPK67_001404"
     CDS             complement(8004..10130)
                     /gene="cdhC"
                     /locus_tag="KPK67_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBG2458397.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(10187..10993)
                     /locus_tag="KPK67_001405"
     CDS             complement(10187..10993)
                     /locus_tag="KPK67_001405"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG2458398.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(11043..12410)
                     /locus_tag="KPK67_001406"
     CDS             complement(11043..12410)
                     /locus_tag="KPK67_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBG2458399.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(12441..13385)
                     /locus_tag="KPK67_001407"
     CDS             complement(12441..13385)
                     /locus_tag="KPK67_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBG2458400.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(13405..14175)
                     /locus_tag="KPK67_001408"
     CDS             complement(13405..14175)
                     /locus_tag="KPK67_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2458401.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(14231..15616)
                     /gene="lpdA"
                     /locus_tag="KPK67_001409"
     CDS             complement(14231..15616)
                     /gene="lpdA"
                     /locus_tag="KPK67_001409"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG2458402.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(15660..16541)
                     /locus_tag="KPK67_001410"
     CDS             complement(15660..16541)
                     /locus_tag="KPK67_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBG2458403.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(16577..17251)
                     /locus_tag="KPK67_001411"
     CDS             complement(16577..17251)
                     /locus_tag="KPK67_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBG2458404.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(17288..18160)
                     /locus_tag="KPK67_001412"
     CDS             complement(17288..18160)
                     /locus_tag="KPK67_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBG2458405.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKEGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(18252..18884)
                     /locus_tag="KPK67_001413"
     CDS             complement(18252..18884)
                     /locus_tag="KPK67_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBG2458406.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(18989..20665)
                     /locus_tag="KPK67_001414"
     CDS             complement(18989..20665)
                     /locus_tag="KPK67_001414"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBG2458407.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(20914..21693)
                     /locus_tag="KPK67_001415"
     CDS             complement(20914..21693)
                     /locus_tag="KPK67_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2458408.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(22003..23922)
                     /gene="cooS"
                     /locus_tag="KPK67_001416"
     CDS             complement(22003..23922)
                     /gene="cooS"
                     /locus_tag="KPK67_001416"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG2458409.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(24529..25440)
                     /locus_tag="KPK67_001417"
     CDS             complement(24529..25440)
                     /locus_tag="KPK67_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG2458410.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFAYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(25556..26134)
                     /locus_tag="KPK67_001418"
     CDS             complement(25556..26134)
                     /locus_tag="KPK67_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG2458411.1"
                     /translation="MNINEFLTHVNQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRAIDEIPE"
BASE COUNT         7696 a         5364 c         2963 g        10196 t
ORIGIN      
        1 ggaaaacgat aaacttatga aataataact ttaaacgcaa aatagtattt ataattacta
       61 agtcaatata atagagaatg tctgtagatt tgtgtaaata caagcttatc tacaatctta
      121 tataagaatg tttatataaa taagatagat taatcctaag tcctattatt ctattgaatt
      181 ttattattat tttatttaga ttctattgtt attttactta gaacttatta ttattttatt
      241 taagttctat tactattttc aatcatataa atttacaaaa gactgtacat aaatttattt
      301 tatacaaact tatctacagt cttttgtgtg aatatatcta aataatatcg tatttattta
      361 tttcctctgc acagtgagaa tcagaaccta aaactaatgg aatatcgtat ttttttacca
      421 actctaataa gtatccatct atataagact ctttgcagta ctcttttcta tttccagaaa
      481 cattataatc aagttcataa cctttttctt taattaactt tattaactct tcaagaattg
      541 tatttccttt ataatcgtat gaaaatacct gattaaactt tctaactaaa tttaaatgac
      601 ctattctttt tggtttatgt atacctaaat ctgaatttac agcttttatc aatgtttcat
      661 aatacttatt ataaacagct tcaatacttc ctaatttgtc gattaaattt tgaaactctt
      721 ctacactgta atctacacaa taataatcat catcaatttt aattatatgt actgataaaa
      781 gagagtcttc taattccttt ccatatttat ttaaattctc ctttatccct tcttcgtatc
      841 cttctataaa atccacttcc gccccaacat ttatcttgat tttaccctca tacttttctt
      901 tcaacttctt gacatcatta aaatactttg gtaaatcttc aatactcata caactatcat
      961 tttcaggagc agggtctcta aatccatttg catacggaaa atgttctgta aatgtcattt
     1021 ctgttattcc aacatccatt gctctttgta catatttttc aaaatcatcc ttacttccat
     1081 gaggacaata aggtgaatgt atatgaccat ctttcataaa taattcactc cttttttaaa
     1141 gaaaaaatct aatataaatg cataactctt ttaacttcaa aataatattt ttaatcatgc
     1201 taatttttat tatatctaat attagagtaa ttttaaactg ccttttaagt atcttatcat
     1261 tagtactatt ttcaacttaa cattaatatt attttttaac ttaacattaa tactatcttt
     1321 aaatagtaat actcaatata taattactta acataaaata actcaaattg cttgatgaag
     1381 cttttgcagt atttaaagaa tatataatta tttaacatta aattatccaa tcttgtaata
     1441 aaatttactc attaaagtat attacagaaa atacctcaaa ataaactttt tagttcatga
     1501 gatactcttt tttacatgaa attagatata tttttatcat ttcaacaatg aaagggaggc
     1561 ttatatccat ctttagacaa ccccttagat aaaattttta ataattaaac atcacaaaat
     1621 ataaaaatcg cttatatcaa acgtataagc gattaattat atttacatat gttttttagc
     1681 ctctaacaac attttattag cttcttccat atactctata gccttttcaa tactctttgc
     1741 tgtttcttct tttccaatag ctctagcctt ttcaacccac tctctaaatc cttcttcatg
     1801 agtttcattg tgatttatcc aatgtactaa aagtattttt aaagtttttt catccttaga
     1861 ctctgatgat tcctcatgac tatgttcgtg gtcgtggtcg tgagcatggt catgtggatg
     1921 ttcatgacat atatctccat gagtatgttc gtggcagtgg tcatggtcat gatgtccatg
     1981 atgatatctt acaatctctt tatcaaaaat cattagttat tccccctgtt attttatatt
     2041 aaactctttt tttataaggt ttacagaatc ttttacaagt agtgctaatg gtttaccttc
     2101 taatccaact aactccacat tttctggcat aataggtaaa agtatcttaa gtgcctcact
     2161 gtctgcaata gcttcactta tattacaagt aacttctccc atcatagaat taggtattat
     2221 tacagaaata ggtgcaacta ttatatttgc ttttttagct gatactacta tagcattttc
     2281 ccctgtagct cctttattag catgagcttt catcatagaa ctagttgcta tagaattagt
     2341 ccctagtgca taaatttcta catatgtagg taactcttct ctcaaagaag aaactatctg
     2401 ggctccaata cctccaccca ttccatctat cactgctatt atcatatgaa attcccctat
     2461 taattaattt tcttgtatta ttatcttatg gtccatcaat cttatttctt taattatccc
     2521 atctattatc ttttgctctc ctaataaatc cgttaaataa atctttccat cacaagggtc
     2581 tatattaacc acattttcca taaccttttc taactcatta ttacttttac aaatgaaagc
     2641 tgatgattca cacatataaa tctcctccta atatgatttt tattaaaaat caaaaaaaga
     2701 ttcccttagg tatttcaata caccaaagaa accttcatga tttcagtttt tgtatattat
     2761 tataccttaa gtatatcact ttaatttatg taaagtcaat taaagttaac gctttttatt
     2821 atactcttca ttcttgtatt tttcatatag ttctttaact aattgcttct cataatcagt
     2881 caaatcgccc tccacaaact caacattaaa ttgttcagct aatcccttta ctatgttttt
     2941 ctgtagaata tctatgtcta tttttctgcc aatttcattt tctattccac ttgctttttt
     3001 agctgtaaat ttcatagctc tttctttcaa ttctggtgtc ttagttttta ttaatttaaa
     3061 caatttttct acatcaaaat ccagtattat tgaaccatgt tgtaataaaa caccatcttt
     3121 tctactttga gcacttccaa ttaccttctt attattaata gtaacttcat atgatgcatg
     3181 tgcattaaaa catgcagctg ataaattttc cctacttatt ctctcgcctc tatttaaatt
     3241 atctgtttct ataccactta gattcaaccc tttaactaga ccttcactta tatatctata
     3301 agataggttg atgctcttat cgattaaagg attatcttct cctataatta tactataggt
     3361 tagctcatta tcatgtaata cagctcttcc acctgtaatt cttctagtat agtctatatt
     3421 catacatcta cacttatcta aatctatctc atcttccaat ttctgaaaat atcctatact
     3481 caaacaagct ggttcccatg tataaaatct aagagttggc ttattatgtc ccttcttata
     3541 tgcagtaaat atagcttcgt caattgccat attcattgct ccttcatatg atttattatg
     3601 tataactctc cactgattca tgattattac gcccccattt ttagtataaa tatatcctag
     3661 aaatcctcta tagatttatt tctcttattg ctaaatctat ctcactttga tttaaaggtc
     3721 ttgaatagtt gtatatagta cttcctggtc tttcattata gtatggtcta ttacaatcct
     3781 tacaacctct tatttcaaaa ggatagcctt tgtttatgtc ttttaaaata tcattgtcaa
     3841 gctttatact atttaagtat ccatttttaa acttaaaata ctcttttgga taacctttat
     3901 ttatcatata agacattaac tgtacccttc tataactttc tatgcttggt tgatttatct
     3961 tttccatttt agttcctcta actggagtaa atgcaaataa actaatcgtt acatcatttt
     4021 cctttaaata agtatataaa ttgtatatat cttcatgact ttcacccata ccaactatta
     4081 tatgtgtact tattttattg ggatatgatt ttgacatttc agttataaat tttaactttt
     4141 catcatagtt atttcctttt attttttcat atagttcttt atttgctgca tctattgcta
     4201 ttcctatttt atcaactcct gctgaaaaaa acttctttat ctgttcatca ttttctaatt
     4261 ttgcagatag tgaaattggc atgtctatct taccacttat atagtttata aagtctaaaa
     4321 cagagttatg tgcttcttca cttgccattg attgaataca tattctcttt atattttttc
     4381 ctttataagc ttttaaagca tctaatattt cttcttttga aaactctggc caaattactc
     4441 ttgataattt atcttttcta gtactacttt ctattgattg agaacaaaaa ctgcatttat
     4501 ttatgcattt ttctccaatc attatatatg ctgtggtagg aggtatatcg ctttttttgt
     4561 ttaatatccc aagttctatt gctgtcccaa ctgatagtct aatcataaaa cgcaacactc
     4621 cttacactca tttattgtca tattcatttc tatagcctta ttctttgcag accttgatgg
     4681 taatactatt ctattgactc cacacattat agataattgg tctagttctt ttctatacac
     4741 tcctcttggt ctcatacacc ctagattaat ttcagtatct ggtaaattaa ttctagcttc
     4801 acataaaata tctgctaccc cttctaactc aggaggttct acattttcat actcagttcc
     4861 ttttgttgga ataaggacta taaatgtaag cttattagga ggatttttct gtaggtattc
     4921 tataatttca tattcaccct ttatttgtcc tccttttaaa ccaatacata tatgtggtgc
     4981 tacttctgta tgttcttgta ttgtattata gacttcaaca taatcttctt tactcttttt
     5041 catcttataa acttctctta ttgtctcatc atcaaacact aaatcaaaag aaactatatc
     5101 taaatactta cacaactcta caatgctatc tttatccata agacctaaat gagcattcaa
     5161 tctatatcct tgctctttta agtttttaat ggtattaata tgagtattta tagggacatc
     5221 tccctcataa ctacaaccgc cactcaatag aaagctagtt atattccttg attgtacttt
     5281 ttcctcatat tcttctattg gcaccatatt gtttaagtag tgaccgttgc aatgggcaca
     5341 tttcaaacta cattgattgc ttgtagtact aagagctagt gtctggttag gataagcaaa
     5401 ctctatttta tttccaaaat tctttaattt aatatcaaat gctattttca ttttttcaga
     5461 tagattctta ttcatagttt attccttctt aagtacattt tactcttcta gtaatttttt
     5521 tgctgttggg aactgctcta tattcgtatg tggtaagaaa catgcagaaa taaattcatc
     5581 catataactt ggatatacac taagttcaac ataagttatt tgattaccta tttcttcaag
     5641 tttatttttt gcatctttac ttataagagc taagtaagaa ccaacaagtg aactattccc
     5701 tatataagta aatttttctc tatcaatatc aggaagtaac cctataatta ttgagttttc
     5761 tatgtctagg ttgtttccta taccacctgc tatataaacc ctatctaata tactaaaatc
     5821 catacctaaa ctatctacaa gagtgtatgc gcctgaatat atagctcctt tagctctgat
     5881 gaaattatct atatcaactt cattgactac tatatcattt tctaaatcaa attcttcttt
     5941 aaatgctaat acatactctc ctatctcatg ctcattaaat ctaactctct tattatctaa
     6001 atccctgtat attttacctc ttctatctat tacaccttta gtaatcattt gacagattaa
     6061 atcaattata ccagaaccac atataccaac tggtgcacat tcatcaataa tctttaatgt
     6121 tggctctaaa gtttctttat caataattac tttctctatt gctcctgctg atgctctcat
     6181 tccacagctt atccctccac cttcaaaagc aggtcctgct gaacatgcac agctcatcat
     6241 ataatcttta tttccaaata caatctctcc attagttcct aaatctataa ataaaacatt
     6301 ttcttcactt gaccatatcc ctgctgataa tactcctgct gttatatctc ctcctacata
     6361 acttgcaaca gatggagcta agtacacata tgcactgtca tttacaaaaa gaccaacatt
     6421 ttcacccata agttttggag attttaaaaa tggtggtatg taaggttctt gtcttaagaa
     6481 gtctgtatac acacccaaga ataaacttgt catagttgta ttcccagcag ccactaatgt
     6541 aactacatct tccttatcta ttccatttct ttcatatatt gtttttaata gtggatttat
     6601 agtttcctca actatagctt tatgaagtgt ctctaatcca tttttcttag ttgaatatat
     6661 aattctattt ataacatctg ctccatattt tatctgtgca tttccagaag atgctttatc
     6721 tactacctct tttgagtata ggtctactaa acaaacaact actgaagttg taccaatatc
     6781 tattgcaact ccgtataagg aattttcttt atttccttgt tctatattta tgattgtaag
     6841 tttcttttgt ttttgaacat atgttattgt aaccttaaaa tcactctttc taaatacagt
     6901 tggcattttt ctaagtatat ccaatctaaa atctatttca ttatatccta ggttatttct
     6961 aacatatctt tccaatctat ctacatcact tatattatca tctaaatttg gttcttccat
     7021 ttctatgtat ttctttttta tatttgtttt aaattgaagg ttatgttctt caattatttt
     7081 tttagctctt tcaaaaattt ccctatcctc ttttttatta ctaccttcta tcttcatacc
     7141 atgcatagaa gatgatactt tagaaggaac ttcaatttct atatctgata ttacctttgt
     7201 acaacaagct agtatataac cttgctccca ctcatcatct gttatatgtc ttgttttttc
     7261 tgtatcaact tttccattca ataattttac tttacacttc ccacaagaca cgtttccatt
     7321 acaaggagca tctatgaata tatctgcatt tcttgcaacc tctagtaata tatccccctc
     7381 attacaataa acctctttat tgtttggtgt aaaacttact tttatcatgt tatccctccc
     7441 aatttgttct atatatgata aaaggaaagt atagctaaat ttgcgtgcac tcaaatttta
     7501 ctatactttc cccaattata ataatatctt aatctttaat tattctaatc cagcctcgta
     7561 ttctttatca cttaataacg tatcaagttc agccacatca gctagtttta cttttaatat
     7621 ccagttttca tatgggtctt catttataca ttctggctca tcttctaatt tttcattaac
     7681 ttccaatacc tcaccattaa caggagatat taaatcagaa gctactttag aagactcaac
     7741 tactccaaaa tctactccct tagttacagt atcttctact tctggtgttt caacaaacaa
     7801 tatttctcct aattgatctt gagcatagtc tgttatacct atatatacaa catctccatc
     7861 aattactttt acccactcat gatctttaga gtattttaat tctggtaata atttcatttc
     7921 gatagacccc caatattata ttattgatta aattacttaa atcatattaa tattatatca
     7981 tattatttta aattttccat caattacatt acactttcca tagctaatgc tggatgacct
     8041 ttttcttcta agaaagctaa tactccttca gaatcgtcag ctatagtttc atcacaaacc
     8101 atatcacaga agttttctat tccagtcatt tctttaactg tagcatttaa tttatcagct
     8161 acataatctt ttaattcttt tggcatccaa actattcttt ctggtccacc ttctgcataa
     8221 gcgaattttt tagaagatat gaaatgtctt ccgtgtccca taaatcctgg agtttgaact
     8281 cctccacctg tcatagaagc aagttctccg aaagtcatac caactggagt aacacttgca
     8341 aattctctgt taactacaac aaatccattt gcttctggca ttataccaca gatacattcg
     8401 aagcatccac aagaagtcat tgggtcttct agtatagagt ataatgtaac agattcaact
     8461 gcaccttgag atatttggtt tacagtttca tttactgaat tccatacacc tgttctatca
     8521 tctaagcact cgcctttttc tattggttga caaggacctg ttgggtctaa ttctttagta
     8581 gccttagcat ctaaccagct aactgctcca caaagaccaa gtctttcagg agtaacaaca
     8641 caaacgtgag ctggtgcaaa tgattgacat aagttacaag aatagaaagt atcaacactt
     8701 tcatcaacta atgaagctaa tctttcatct ctagcattgt acttagctat tgcttcacct
     8761 tttaattcag aaactttttc agcatctgta attatagtta tttcacactt atcaactact
     8821 gattcaaatt catctaacat cttagcatat aaaacttctc ctatatgctc aagtctaaat
     8881 cctttatcaa atgcatcttt agagattctt acccaagcca tatctctttg tccaacgtgc
     8941 attacacctt ctatatagtt taagaagtag tggaaacgtc tttcaagaac tggctcgaaa
     9001 tcttcttgca tatttttacc agctatttta actattacag caagtggtaa tcttaataca
     9061 ccatctgcat caacttcatc tatatttggt cctattattt ctatcttatg gtcttcaact
     9121 tctgttgctt ctttcttaac aacaagctcc caagcttcag ttctgtttcc accaaactca
     9181 gcaaacatat cgctctttct aattctctca ccttcaaatg ctgctgcaaa tgaaactggg
     9241 ataggtattt cagtaacttt tatttttatt cctctagctt ctaatgaagt agctactatc
     9301 ttatcgtaat ctttttgagt taataggttc attggaactt caagaactgt ttggtcagtt
     9361 ataactggga atcctaaagc tattgctcca gcacctgcag atacaactaa ttcacttaat
     9421 ggtccaaatg cattaacaaa tgcaggaact ctattagctg tgtactctaa taatccgttt
     9481 aaatcaccag gagttaatcc accgaatatt aatgctgctc ttactgcaac agatacaacg
     9541 tgtataacag aagttacatc atatcctaga ggtataactc ttagctcaag acccattttt
     9601 actccagctt ctatagcttg gtctataact ttaccaacta agaaagttag taaaccttta
     9661 gattggtaat cttttataac ttttgctgct gattctgcat ctggacactc accaagaaca
     9721 actgcaacac caggtatatc tcctgtaact aatggtacac caagtgatct gattataggg
     9781 tcagttatat gacctgcaca tggctcacta tatggagcat ccatagttga atattttaat
     9841 gcttcaatta cttctgctgc taaagctgta gctaaaccag cattaaaagc atgttctagt
     9901 aaatgagttc tatttattaa agattttact acttctaaag caccttctaa gtcccctaaa
     9961 gtgttcatct tttgtcctgt tgcagcatat atacaaggta atgaatatgc tgtatcaggg
    10021 aaagcaactt tatgctcttt tccattcttt tctatagctt cagctaacat agcttgagcc
    10081 gcacctaaag cttgttctga ccctgtaaag attatattat atagattcat tctatttccc
    10141 cctttaatat ttacacctat tttttattaa ctatacccta aaaaccctag aaagcaaatc
    10201 caccttcatt taactctaag aaagagtctg catataaaga tgccccgttg attatgtcac
    10261 aagattttac agttttcatt aattctgggt cacatggatt cattatagct gaactaaatc
    10321 cattagccat tgccattgct aagaatgcac tatctaaaac aggtctaaca tgcttaggac
    10381 atccattaga tacgttagat aatcctcctg tagttaaaag tcccatctca gtcatcattt
    10441 ttatagcttc taaaacttct acttgttttt cttgcatacc ttttataact aagcataatg
    10501 ggtcaaatag tatatcagtt ggctccattc ctaacattaa gcctttttct aatatttctt
    10561 ggcagtaagc catacgctca tcattatctc ttgggatacc ttcttttgca cataaaccta
    10621 taaccattgc ttcgtattca gctgctatat ctattaaatc aaatcttgag ccagcatcag
    10681 cagagtttat tattggtttt gcattagttc tatcataaac tttaagtcca gcttcgatag
    10741 cttttttatt agctgtatct aatgctatag gaacattgtt gaactcagat tgaagtaatt
    10801 tgattcccca tgtcattatt tcttcgccat ctctttcggc aggtcctata ttaaaatcta
    10861 tatagtgtgc tcctgcttct aattgctctt ttgctctttt taagattgga gctgggtctc
    10921 tttctgctaa tgcttttctt attgaaggtg atatacaatg tattctctca cctataatca
    10981 taaatttttc catttataat gtcccccttt aaatttaata ttaatagtta gttaatattt
    11041 tattagtttg tttggcatgc tttttcttgg tatcctttta agaacttagg taattccata
    11101 gattcctctg gtcctataac tacattccat cctggtaagt tatcttctat atcaccttta
    11161 agtactgcaa cttttcctgg aatgataagg tctttacaat tagttacttc ttctacctta
    11221 ctttctttaa tgaaagcaga gattgagttt ccaccaaatt taccagcagc ccaagatgta
    11281 agaactgaat atccacctgc atctggtatt actaaccata ctggaacttt tgatctttct
    11341 atatcaccag caactatgaa gtaagttaat gcaaagtcaa cagtaactaa tactggagaa
    11401 ttttcatctg ggttgttgat tgggtaaatc tttggttcaa ctctcattgg tctttgtggg
    11461 tcagtgtata tgttttgtct tagtgcaaat aatggtaatg ccttagcata actaatatca
    11521 tctattacta ttatagaacc gtattttata gtgaatattg atgataaagc aacttccatc
    11581 atagggtttg aattagataa tctatttgca aatactattg aaggatatcc aaatgttcta
    11641 tcttgctctt ttaaagctgt tcttctaact tgtatagcat tagtgtaagt atcttttatg
    11701 ttttctcctg ttacatctaa tactaattct ttatatccag cggcttgaat taattcaact
    11761 gtttcatata attcttctaa gctaccagct tttacaccta atggtaaact tgctccttta
    11821 actacttcta tcatatcttt gtagttctct ttagttgctc catataccat tggtctagca
    11881 tcttttaata cttcaacagc ttctttaact acttgtgcat catcacaagc taaaatataa
    11941 gctacttcta atccactgcc ttttatttta tctattaatt ttaaatacgc atctttatct
    12001 ccacaatact ctaaaacagc catttcaact ttcatttgtt caccaattct tacatagtca
    12061 acttttttca tgttagctat tttagcatct actgcttcat cactcataca agtacagaat
    12121 gaaactgcat atctatttct acttactaat gttttttcat gtctaaataa tactgtttct
    12181 ccacctagtt catactcaga tgcaccagca ccaactttta aagctttcat taaaggtgca
    12241 gtagcctctg ataattttgc tatagcgtca tcagacatat gtgggcattt acctacttct
    12301 acagctcctg aagcaacctt catagagaaa gccatacaag ttgggaatcc acaatcctta
    12361 cagtttttct ttggtgttaa tttaaatata tctaaagctt ttaatgccat tattttttcc
    12421 ccctaacctt aacaatttaa ttaagctaat gcgttaacca attcttttat agtttctatt
    12481 gattttggat gacgaagtat aactgcgttt gaaccaccta ctaaaacact tgctgcagtt
    12541 gaaacttcca ttgctatagt tctttcttct ggacaacccc aatctggctc atcttctatt
    12601 ggagctatag cttctttaac atgacctact tcaaaagcta ctggtgttat gataggcatt
    12661 tgtaatgtct tatcattttg accaaatgct gcaagtctaa ttctatccat agtagatgca
    12721 acgtactcat atccataacc aactgctgaa catccaacat tcataactat attttcacct
    12781 ttaacgccta gttgagttaa caatacgttt aattgtttag ctaggtttat atcaacagaa
    12841 gattcagctc ctactttatg tgcataagcc ataccagctg atgctcctac tcctttgtag
    12901 ttatcttcta ctgctgacat aaataagcag ttatgtccat ccaatgtttg agctaatttt
    12961 tcaaataatt taccatcttt ttcatgattt cctgaaccag ctacaactaa aggtaatttt
    13021 attgcttcaa ttactgcctt agctacatca gcacattcat caactgactt atctaatcca
    13081 tttgggtcag aaccatcaaa ttttaaacaa ataaaatctg cttgtgtatt agcttcaaca
    13141 tatttagccc actctactgg acaattagct acatctttgt ataattcctt atatgaatca
    13201 gtccagcttt caggataaac gtctgatatt tgtataccta tttttggaga attacctact
    13261 tctccatcaa aactataaaa aggtaataca ttttccccac ctaatttaat tgctttttcc
    13321 cctattccta cttcaacttc tgatattttt ccagaatatt tttgagtaga cattttaaat
    13381 gccatatttt tttccccctt aagcctattt taattctaat ttagcaaaaa tttctttcat
    13441 agccacttta gattttgaat cttcaggtaa attaactaaa ggaatacctg tagaatcata
    13501 ttcatatatc aattcatcca taggtacaac accgattagc tctaggttat gcttttcaat
    13561 ttcttcttta acaccatcat tcataacccc atttggaact ttatttacaa tcaaaagtat
    13621 tcttccaaca cttaacttta gttcctcagc taaatctctt attctagcaa cagcttgtat
    13681 gctacgtctt gagcaatcac taaccaataa aagtgtatca acatgtctag tgacttttct
    13741 acttagatgt tccataccag cttcgttgtc catgactaag tatttgtaat gaccagatat
    13801 tttgtttact tgttctctaa gtatcccatt tacaaaacag taacatcctt caccttctga
    13861 tctacccatt actaataagt catatccttc gccttcttcg attatagaat ttaatctaaa
    13921 ttgaagatat tgtgcctttg tcataccacc agggaatgca ttgcctaact tttctctttg
    13981 atttacttct tctcttattt ctcctattgt tgcttcaact tcgataccta acacttcatt
    14041 tatgttagca ttggcatcag catctacaac taatactggt ccctttttgt ccttaaccaa
    14101 ataatcaatc aaaagccctg tgagacttgt tttaccagtt ccaccttttc ctgcaacagc
    14161 tatattgtat cccattttaa gacttccccc ttaaatctag ccacagagcc ttatgtactc
    14221 tgtctgtcta ttataattta ggtacagaat gaacacattc tccatgtaca tcatgaagtg
    14281 cttccattag cccttcagat aaacttggat gtggatgtat tgcatcccct acttgttcta
    14341 ctgttaatcc tagatgaaca gcaagtgaca actctgtcaa taaatctgta gcatgaggtc
    14401 ctactactgc tgcacctatt atcttatcag tttctttgtc agctataact tttacaaatc
    14461 cttggaaatg tcctatagct tgtgcttttc caagacctct aaaatcaaat tgacctacat
    14521 tgtattctac accttcagct tcaagttgct tctcagtctt accaacacca gcaacttcag
    14581 gttcagtata aacacatctt ggaattgctc tataatctac taccttagtc ttacctaaag
    14641 cattttcaac tgctacgata ccttctttag aagcaacatg tgctaggaaa ggagtatcta
    14701 ttatatcgcc tattgcgtat ataccttcta cattagtttc aagatgttca ttaactacta
    14761 cttttcctct ttccatttca attcctatat cttctactcc agaattgtca aggttaggtc
    14821 ttctaccaac acatactaat gcatattgtg cttctataac ctttccatta gaaagagttg
    14881 caacagcctt accatcaact acttcacaag tttgtactcc aatacctgta ataaccttaa
    14941 ttttatcttt cttaaattgt ctaagtagtt gtttagctac atctttgtct tcatttaata
    15001 gtatttggtc aaccatctca actatagtta cttctgttcc taaggctcta aagaactgcc
    15061 ctatctcaca tcctataact ccaccaccta ctattaacat agactcaggt atctcttcaa
    15121 gtccaagaac ttcatcacta gttattacaa cttttccatc atatgggaac attcttggta
    15181 caactggaac agaaccattt gctagtatta tcttatctgc ttttatagtt tctactgttc
    15241 catcatcttt agttacttct atagtgtttt tatcaattaa ttttccaaaa ccattaacta
    15301 agtttactcc tcttttttca aataagaact ctataccact tattaattgg tttacaactt
    15361 tattttttct ttccattatg gcagtgaagt ttggcttaac tgtaccatct atttctatac
    15421 caaaatcctt tgcttctttt acagtattca atactcctga agaagcaaga agtgctttag
    15481 ttggtataca tcctgcattt aagcaagttc ctccaactct tcttttttca acaactgtta
    15541 catctgcacc aagcatagaa gcttttatag ctgctacata tcctcctggt ccaccaccta
    15601 ctactactat cttcatcttt aattccccca aacctaatat aatctacaat cttgcaatac
    15661 tataaacctg cggcatccag tatagcaggt atattctctt ctgccccaat tgccattata
    15721 tgaacaccat cacataaatc ttcttctttt aattgtttga taagttcccc tgccattttt
    15781 attccttcac ttacccagtt ttcttttcca gctgctctta atctttcgat ttgttcatct
    15841 ggaacaaata ttcctggtac atttgcagtc ataaatttag ccattcctgg tgattttaaa
    15901 ggtactattc ctgctaatac cttgcaatcc atatcccttg ttaattctct aaactttctc
    15961 attgtattta tgtcataaag tgcttgtgtt tggaaaaatt tagcccctgc ttttattttc
    16021 tttttcattt ttaataattg aacttctatt ggagaatatt ctggagttac tgatgctcct
    16081 agataaaaat ctggagaacc ttttaactta ttaccagcca tgtccgtacc tgccattaaa
    16141 gtctcagcag tttgaagtat ccccacagaa tctaaatcaa acactccctt agcttgtgga
    16201 tggtctccta cgctagtatg gtctcctgtt agtgccaaca gattgtttat cccaaataca
    16261 cctgctgaca acatttctcc ttgtatagct attctatttc tatctctccc tgttatttga
    16321 ataactggct ctaatccagc atcttttaat aatttacatg tagcaagaga agttgctctc
    16381 ataactgccg attgaaagtc tgttacattt gctgcatgaa ctcttccaac taacggcttt
    16441 gcacattcta ttaaatgtga aaggtcagtc ccttttggag gagccatttc agtagtaact
    16501 gcaaattttc cactttctaa tgtttctctt aataagctca tctctaatcc cccttttaac
    16561 cttctaaaaa aaacttttat gaatttgctg ctgcttcttt ctttttcttt gcacttaaac
    16621 ttcttggatt attttgctta gaatagtctt ttggtggtct tatttctgct aagttatcta
    16681 attgtcctat agcttctagt ctttcatata tctttatcca agcacaatca ttctctgagt
    16741 ttacttcaca cttaccattt ttagctccac cacaagcccc attcataaga cctttagcac
    16801 acatagttac tggacatatc ccacctgtcc aaccaagttc acagtctcca caagccttac
    16861 aggcttcttc atattctcca actctttgta cttcccctat aaacaaagta ttgttggctg
    16921 gataaactgg cttatctttt aaattcttaa ctattgtctg tgtaccatct ccacatgcta
    16981 aagataaaac tgcatcagct tccttagtct cttcttttag agcctttaaa tcctttttag
    17041 actttaaaag gttacaagca gggtcaagca ttacatatcc taagattttt ttgccttcac
    17101 cttcaagagt ttccttcatt tttaaaactt cttcttcccc accactttta caagttgctg
    17161 cacattgatt acaaccaact aaaaccagct tgtcaaaatc ctttaagtac cctagcactt
    17221 cttctaatgg tttattttca gaaattatca tatctacttc ccccctaaac cttccacata
    17281 tatatatcta tttattgttt tgtaatttac aagccttaac tacatgtttt gcaagtatag
    17341 atgttgtaac tgaaccaacc cccgctggaa ctggagttat cattgatact ttgtctaaaa
    17401 ctgcatttgt atcaacatct ccacataaat ttccttcttc atcaacattt ataccaacgt
    17461 ctataactac cgctccctct ttaacaaaac tttcatcaac catcttagct ctaccaatag
    17521 ctgctattag cacgtctgcc tctgctgcta cacctgatag atttttagtt tttgaatgac
    17581 atattgtaac agttgcatgt tcacttaata aaagcattga tactggcttt ccaactacca
    17641 ttgatcttcc aagaactgtt accttacttc cttttaaatc aacattatag tgttttaata
    17701 tctcaactac tgctgtaggt gtacatggtg gaaatccact tttatcccct tccattacct
    17761 tagcagagtt tattggacta aaacaatcta cgtctttttc cggtgcaata acatatttta
    17821 taacttcttc atttaagtgt tttggaagag gtctaaaaca taatatacca ttaacatttt
    17881 tgtcatcatt aactctcttt aatacatcaa tatattcctc ttgtgttata tcctcagcta
    17941 actctaacac ttctgttgtt atacctatgt tttgacatct ctttagtgct cctctttcat
    18001 aagataaatc atcacttctt gccccaactc taactattgt tagtttagga tttatacctt
    18061 ctttaattag taaatcgact tcttttatta attcttcact aattttatca gctacaggtt
    18121 ttccttttat aatttgtcct ttagttgaca ttccttccat acattcacaa ctcccctaca
    18181 agcaaactta aattaattta ttctatatac cacatattaa taacatagta tctactttca
    18241 tcatttaaaa attattttcc aagagctttt tcaactttgg cataaacttc atcacaaatt
    18301 tttacaccat cttcaactaa cttatctact tctgttttta ccttattaac atattcttta
    18361 tcttgtatag aatttatatt tataactaca tttaattgtc caccaagtat agcagctctt
    18421 aaacactgaa ctcctacacc aacatcactt atagcaagtc ttgaaccttt atcaactaaa
    18481 tcttcatgta acttaatagc ttcatagcaa actcttacta tgtttaatgg tacttcacaa
    18541 gcaactttta atgccttttc cattgtttct gcttttattc ttttttcttc ttctgtttct
    18601 ttaggaagcc cgtaagcttt tgataatggt aagaaacatt ctgcatcatc atctatcatt
    18661 tttaataaat ctttttctaa ctttcctgcc ttacttaata tctcttttac actttcttca
    18721 tactcagcat acttcttctt ccctattgta aggttacata ccatactacc taaagccata
    18781 cctattgcac ctactagtgc agcagctccc ccaccacctg gtactgcttc tttggaagat
    18841 agtacttcta caaaatcaac acaagtttta tctgctattt tcatgataat cctccttgaa
    18901 aggatgtagg aaaatatctc aaattgttcg agatatttta cgaactattt taagatatct
    18961 cctactcttt atttataaat taatttcctt agaataatcc agctattact ccattttcat
    19021 caacatctat tttttctgct gctggaactt ttggtaatcc tggcatcttc attatttctc
    19081 cagtaagtgc aactataaat ccagctcctg ctgaaattgt tgcttgtctt acagttattc
    19141 taaatcctgt tggtctacct aatttagttt ggtcatcagt taaagaatat tgagtcttag
    19201 ccatacatac tggaacttta gtaaatccaa gtttttctaa gttagctatt tccttatcag
    19261 cttcaggagt ataatctaca ccatctgctc cataaatctt agtagctata gcatttatct
    19321 tgtcttttat agataaatca tcttcatagc agaatctaaa gttattttct tcttcatcca
    19381 ataatcttaa aacttcttta gctactgcaa ttccaccttc tccacctttt gcccatactt
    19441 cagaaagagc aacattaaca cctaattcct tacacttgtc ttcaacaagt ttaagttcag
    19501 cttcagtatc aagtgggaat ctattgattg caacaactgc tggtaatcca tatacttgag
    19561 ttatgttttc aacatgtttt aataagtttg gaagtccttt ttcaagagct tctaggtttt
    19621 cgttatttaa gtctgcttta gcaactccac cattgtattt taatgctctt acagtagcaa
    19681 ctattataac agcatctggt tttaagtttg ccattctaca ttttatatct aagaattttt
    19741 ctgctccaag gtcagcaccg aaaccagcct cagttattac ataatctgca aagtgcatag
    19801 ccattcttgt agctataaca gagttacatc catgagctat atttgcgaat ggtccaccgt
    19861 gtacaaatga tggtgttcct tcaagagttt gaactaggtt tggttttaaa gcatctttta
    19921 ataatgcagc catagcacca ttagctttta attgtctagc tgttacaggt tcaccttcgt
    19981 agctgtatcc aactattatg tttcctaatc tttcttttaa atcagatata tcacttgcca
    20041 aacagaacgc tgccattatt tcagaagcaa cagttatatc gaatccatct tgtctaactg
    20101 ctccatcacc ttttttaccc ataccatcaa cgatatttct aagttgtcta tcgttcatgt
    20161 ctacacatct tctccaagtt attttcttag ggtctattct aagtgcattt ccttggtgaa
    20221 tatggttatc aagcatagca gctaaaagat tgttagcagc tcctatagcg tgaaaatctc
    20281 ctgtgaagtg taagtttata tcttccatag gaacaacttg tgcatatcct ccaccagctg
    20341 ctccaccttt aacaccaaat actggtccta gagatggttc tcttaaagca actaatacat
    20401 ttttgtctag tttagcaaat gcatcagcaa cacctatagt agtagtagtt tttccttcac
    20461 ctgctggagt tgggtttata gctgtagtta gtattaactt agctttttta ccagtctctc
    20521 tttttagtag attgtagtca acttttgctt tgtatttacc ataaagttct acatcatctt
    20581 cacctagtcc taattttttt gcaacctctc taatatcttg aggcttagct tcttgagcta
    20641 tttcaatgtc agatttaaat cccataactg aatctcctcc cacaacctaa atagatttaa
    20701 aatataattt ttgtaaaaat tgtattaata tgtaaaaatt atattttata agataattat
    20761 acatgtaaaa ttacttaaaa actagctact aagctattaa aatattcaaa cttaatagct
    20821 agttatttat ctaattactt acttattacc tatctattgc ctatgtctaa cctttaacca
    20881 acttaaaatc ccaaatcaaa atttctatct attttactta tccttaagtg acattaattt
    20941 atcttttatt gctttaatct gttctttcgt tgtttcacta gaatcatatg gagattgatt
    21001 agctctatca gaatctatga catcttgatt ataataaata aatcctaaag attctccatc
    21061 ttctaagttt tggattataa attcttcatc ttccttattt ctaattttat ttcctactac
    21121 aaatacttta tttacaccta tatcatgtcc aagtttctta acttttctat aagtctgtag
    21181 acttctctct cctggttcta caactactat aaaagcatct acaccttggg ctgttcctct
    21241 tcctaagtgt tcaattcccg cttccatatc cataaccact acatctttat tttgtagtat
    21301 taaatgtgag cagagtctct tcaaaagaac atgctctgga catacacatc cagtaccacc
    21361 agaatctacg gttcccaatg taagtaatct tactccattg tattctttac agaaattttc
    21421 agggatatcg tctactttag gattcatctt aaacattttt ccaaatgaac ctacagaagc
    21481 agctgttcta tctgaaacta attttttcat ttctgatata ggtactattg attcatatac
    21541 ttcttttgga aaacctagtg ctaaagctaa attagcatca gggtcggcat caacagcaac
    21601 aactctgtat ccgtcttcag caaacatcct agagagcatt gaagaaaaag tcgttttacc
    21661 tactccacct ttacctgtta ttgctatctt cataatccct accacctttt attactattt
    21721 gctttttagt agttaatttt acagttaaat attttttata ctctatattt ttatacacta
    21781 aaaataaact acagctagtc cttacaggaa ccaattagat ttaccctact agcttttcaa
    21841 agcagcatgt atagttttat tattctttaa tattgtttat attttattta ttcagtatat
    21901 tcattatatt ttgatgttct ttactttaat atttgacttt attcaacttt attcaaccag
    21961 aaacttctta taaaaacgtt tattctataa cttcactatt ttttaagctt cagccttttc
    22021 tgccattttt tcttcaacta atgcttcaaa gtgaacacgt tttttctcta tatcttctat
    22081 tattgtagct gctaactctt ttggatttgt atttacagta aatgtagctc caactttatc
    22141 ttttaattca ctagttaata tatccactgc tcttgaagaa ccagctattg gaggcattat
    22201 tcctaaataa gtgtctatac cagaagctac aacatagcat cctatagcaa ctgctttttc
    22261 tgacatccac tctggagcta tacctacaac tggaaggtca cacatatcca tatctaagta
    22321 attagctgct gctccaacta aatctagtat acgacttata tcaacacaag aacccatatg
    22381 tagtactggt gggattccta ctaatttaca gacagttgca agacctttac ctgcatattt
    22441 ttcagccgca tcagattcca ttaaaccaaa cttagctgct gctgaagctc cacaacctgt
    22501 agtaactact attatgtcgt ttttgattaa ttctttcatt attgttacgt gagcctcatt
    22561 tgaaactcct ttagcattgt tacatccaac tacaccagct gctcctctta atactccagc
    22621 ttttagacag tctgttaatg gcttaactgt tcctgctggg tctatatgag agtttacaac
    22681 tctgtctaat tgatttacta ctgcttctac actataccca actgttgcgc ctgattttaa
    22741 ttcaggtata agtacttttg atttatctct atttttaaag tttaaaatag cttctttaac
    22801 tattgcttta gcatcatcaa gtgcttgttc ttctctaaat tcaatataag ttgaacctgt
    22861 tatcttagct tttggagaag ttgttacaaa tttagtatgg taacaatctg caactctagc
    22921 taatgcaggg aatatacatt gaacgtcaac tattacagct tcaactgctc cagttattat
    22981 tgcaagttct tgttgatgga agtctcctgc tatttttaca ccatgtctca ttgtaatctc
    23041 attacctgta caacacatac ctgctaagtt tattccatca gcaccaactt ctttagctaa
    23101 agctactaag tctggctctt ctgatgctaa aactatcatt tctgataatg ctggctcatg
    23161 tccatgtaat attatgttaa ctttattttc ttctaatact gctaaatttg cctctgttct
    23221 tcttggcact ggagttccaa ataagatatc gcttagacga gtacctatca tagaaccacc
    23281 ccaaccatca gccatagaag ttctaagact catgtgtatt aaactatcta tatcaccagt
    23341 acatcctata tgagtagagt gcattattgt tgcaatttct ctatctatag ctcttggctc
    23401 tatagcgtat tctttccata ttttttgtct tggttctggt gcatttttta taaatcttaa
    23461 tgtcccaaaa ggttttccaa attccattaa tgcaatttct gccacttcat gtgctacatc
    23521 atagatatct ctaccttctg tctctacatc ccattcttta gctaaagtaa ttaacttagc
    23581 ttcgtctctt attttatagt ttccatttgg atcagctaat gctaatgtat gtgctatatc
    23641 tctagcatgg tctgaatggg cagctgtccc accagcaacc attcttgcaa agtttctacc
    23701 tactatagta tgctcatctg ctccacataa cccccttgga gtcttagggc ttattctaca
    23761 tggtcccatt ccacatattc tacagcaaac accttgaaga ccaaaaccac attgtgcctt
    23821 aaagccttct tttctatcat acattgtttc tacgccttct tctctagctt tagctatcat
    23881 cttctggctg tttaaatcaa ttgttaacat tttttcatcc atattcctat cccctttttt
    23941 tgatttttaa aatcccccag gtgactgact tcatgtcata aaataataaa ataaatgctt
    24001 tctaagttga ttatatacta attttcagtg ctttgcttta gatttaaata tttttttaac
    24061 aaaattagat aatacataaa aattttattt tctacactta atttaagttg attttatcgc
    24121 ttgtttttgg ctatttgtta atatctaaac ctactttttt gatacttcag tttaattaaa
    24181 ttatgttttg atttttttaa atcaaatttg aaatttttct agtcattttt atattattgc
    24241 agattgtttg tgtatttatt atctttcact actgtttgga cttattacag atttttactc
    24301 cctaaactta attagtaaaa atttcttaga atagcatttt ttggactaat ttttttgaat
    24361 ttttttattt ttcctacttt aatattaatt atagcttcaa attgaacatt tgtatatatt
    24421 catttgaagc tataattatt ttttatataa aattttttta atacagcatt aatttagaca
    24481 ttttgactat tttttctact ttaattcatt tatttcaagc gatttatcct atttcatatt
    24541 tttagctaca atagccccat atgcaataga atataattta gattcatgtg aatcagctct
    24601 tgacactaaa actataggag cttttgcacc cataattata cctgctgatt tagagtctgc
    24661 catatatgca aaggctttcc ctattccatt accaacctct attgttggaa ccaatatgat
    24721 atctacatct ccagatactt cacttttaaa cccttttatc tcactagctt cttttgaaac
    24781 agctaaatca aaagccagag gaccttctac aattacattt tcaccaaatt ctccttcttt
    24841 acaagctttt gctaacatat ctgcatctac agttgcttgc atcttaggat ttactttctc
    24901 tttagctgcc aaacatgcaa cttttacagt ttccattcct aaagcttttg cagcttttat
    24961 agaatttttt aatatctttg ccttttgttc ataatcagga gctatattca tacctccatc
    25021 tgtagttata agtaatttat ggtatttttc taactcatat accataacat gacttaataa
    25081 actatcagtt ctaagaccat agtctttatt taacacttct tttaatatga cagatgtatc
    25141 taataatcct ttcattacaa agtcagcttc tttacttgat actaatttaa ctgctatttc
    25201 tgcacactct ttgatatcat ccttatccac tattttgata ccacttaaat caaatcctat
    25261 ttcttttgat atttctctta ttttgccttc atttccaacc aatataggag taattatatt
    25321 tctttctaca gcatccttta ttgccattaa aacttcttta tcatgagctg ctgctacaga
    25381 gagtacaatt ttctctcctc ccttaagttg ttctaaaata tcatctaatt tttttatcaa
    25441 aagtaacccc tcccttatat atatttaact aaataatacc atatttgcta taccttttct
    25501 caatttattt tttatgttat aaagaattta tataactaaa agacagttta atattttatt
    25561 ctgggatttc atcaatagct cttaaaagct ttgcaggaac accacctaca atagtatttt
    25621 ctgcaacatt ttttgttaca actgctccag cagcgataat tgcattatct ccaatagtaa
    25681 ctcctggaac tacagttaca ttagcaccaa tccatacatt ttttcctatt gtgattggag
    25741 aaggataagt tgtaccacgt ttctctggtg caataccatg gttaagtgta gccaatacca
    25801 cattatgccc aatttgcgta ttatctccaa tctcaatccc cccctggtct tgaaaatggc
    25861 aactgctatt tataaaaaca ttttttccta ttgtgatgtt ttttccacaa tctgtataaa
    25921 atggtggaaa cataaaaaat gattcatcta ccttttttcc aattaactca gaaaataaac
    25981 tacggacttc ttctggttca tgataagaag aatttaactt acttgtgatt ttcattgctt
    26041 cattactcaa cttaaccata aattcatgta tttctgattt actagaaaca ggttttcttt
    26101 gattgacatg tgttaaaaat tcattgatat tcattttata catcctttca atatgtgctt
    26161 taatttattt gtacattcta aataatattt taataaaaat taatttaatt tatttttat
//
LOCUS       DAEMHW010000040        25466 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEMHW010000040 DAEMHW010000000
VERSION     DAEMHW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.040"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(351..2993)
                     /gene="adhE"
                     /locus_tag="KPK67_001419"
                     /gene_synonym="adhC"
     CDS             complement(351..2993)
                     /gene="adhE"
                     /locus_tag="KPK67_001419"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG2458412.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            3871..5469
                     /locus_tag="KPK67_001420"
     CDS             3871..5469
                     /locus_tag="KPK67_001420"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBG2458413.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(5704..7704)
                     /locus_tag="KPK67_001421"
     CDS             complement(5704..7704)
                     /locus_tag="KPK67_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HBG2458414.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNVDYCLIGSKCSNSFIKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYRILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGVTGPTGNTGVTGEIGPTGATGPTGSTGATGSIGPTGVTGPTGNTGVTGS
                     IGPTGVTGPTGNTGEIGPTGATGPTGVTGSIGSTGATGPTGATGVTGEIGPTGVTGPT
                     GATGPTGVTGSIGPTGVTGPTGATGVTGEIGPTGEIGPTGPTGVTGSIGPTGATGPTG
                     NTGVTGEIGPTGVTGPTGVTGEIGPTGATGPTGNTGVTGEIGPTGATGPTGVTGVTGE
                     IGPTGVTGPTGVTGEIGPTGATGPTGNTGATGSIGPTGVTGPTGATGSIGPTGATGVT
                     GPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIGNSSSITVDANGTFTV
                     QENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTTEPGGGGVLSGYFAGF
                     LFGGTTFTINNFSSTTVGIRNGQSAETAATLTIFRIADTVMT"
     gene            complement(8382..8906)
                     /locus_tag="KPK67_001422"
     CDS             complement(8382..8906)
                     /locus_tag="KPK67_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2458415.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(8971..9723)
                     /locus_tag="KPK67_001423"
     CDS             complement(8971..9723)
                     /locus_tag="KPK67_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG2458416.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNLNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILVSTKYNYELPEDIKKIYDEDKIIVVRPNTQ
                     FAPKDTLNFDDKFCKTIYQEGYEIGKNILDRL"
     gene            complement(9754..11052)
                     /locus_tag="KPK67_001424"
     CDS             complement(9754..11052)
                     /locus_tag="KPK67_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBG2458417.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(11069..13333)
                     /locus_tag="KPK67_001425"
     CDS             complement(11069..13333)
                     /locus_tag="KPK67_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBG2458418.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKSNDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(13588..14406)
                     /locus_tag="KPK67_001426"
     CDS             complement(13588..14406)
                     /locus_tag="KPK67_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG2458419.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(14399..15097)
                     /gene="cbiQ"
                     /locus_tag="KPK67_001427"
     CDS             complement(14399..15097)
                     /gene="cbiQ"
                     /locus_tag="KPK67_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBG2458420.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGCMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(15100..15384)
                     /locus_tag="KPK67_001428"
     CDS             complement(15100..15384)
                     /locus_tag="KPK67_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2458421.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(15374..16126)
                     /locus_tag="KPK67_001429"
     CDS             complement(15374..16126)
                     /locus_tag="KPK67_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBG2458422.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAMPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(16308..16500)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(16632..18404)
                     /locus_tag="KPK67_001430"
     CDS             complement(16632..18404)
                     /locus_tag="KPK67_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBG2458423.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTY
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(18418..19347)
                     /locus_tag="KPK67_001431"
     CDS             complement(18418..19347)
                     /locus_tag="KPK67_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2458424.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            19575..19763
                     /locus_tag="KPK67_001432"
     CDS             19575..19763
                     /locus_tag="KPK67_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2458425.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            19756..20457
                     /locus_tag="KPK67_001433"
     CDS             19756..20457
                     /locus_tag="KPK67_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2458426.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            20459..21382
                     /locus_tag="KPK67_001434"
     CDS             20459..21382
                     /locus_tag="KPK67_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2458427.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            21539..22462
                     /locus_tag="KPK67_001435"
     CDS             21539..22462
                     /locus_tag="KPK67_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2458428.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLHLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            22455..23192
                     /locus_tag="KPK67_001436"
     CDS             22455..23192
                     /locus_tag="KPK67_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2458429.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGAIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            23203..23979
                     /locus_tag="KPK67_001437"
     CDS             23203..23979
                     /locus_tag="KPK67_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2458430.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNTGWYMDAVQPWSTFFVLPAVIALLGGYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKLK"
     gene            complement(24515..25123)
                     /locus_tag="KPK67_001438"
     CDS             complement(24515..25123)
                     /locus_tag="KPK67_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBG2458431.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(25301..25417)
                     /gene="rrf"
                     /locus_tag="KPK67_001439"
     rRNA            complement(25301..25417)
                     /gene="rrf"
                     /locus_tag="KPK67_001439"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         8063 a         4423 c         3289 g         9691 t
ORIGIN      
        1 cttaataggc cccaattttt ttcttttcta aacttattat atcatgataa ataaattagt
       61 caatagaatt ttctgaatat ttttataatt ttttcataaa gttttttata tattatgctt
      121 taaaattata tgtaataaaa aaattttccg actaatttta aagttaattg attaaataca
      181 catattcgaa attaccatta aattactgac tttttatcat ttccatttag aatttaagtt
      241 ataatctatt atttatagtt acttctacgt gtctctaata aaactctatt tatattcaaa
      301 tctaataatt tatatacaaa cccaatagaa cctatgttgc acaataaata ttatttacca
      361 taataaactt ttagatatat ctcttttaat tcactcatca atgggtatct aggattagca
      421 cctgtacatt ggtcatcaaa agcttgttca accatagcat ccaatctttc taagaattta
      481 gattcttcta ctccagcctc ttttatagtc ttaggaagac ctacttttgc ctttagttca
      541 tctattgctt ttataagatt atctactttt tcttggtctg tacttccttt aatacctgca
      601 aaagaagcaa attcagcata tctttgtatg caatcaggat atctgtattg tgagaatgca
      661 cccattttat ttggagcttc tgcacaattg aacttgatta cttcatttat tagtaatgca
      721 tttgcaacac cgtgttgaac atggtggaat gcacctaatt tatgtgctaa agagtgacat
      781 attcctaaga aggcatttgc aaaagacatt cctgccatag tagatgccat tgccattttt
      841 tctcttgctt ctttatcatt ctttccattt ttatatgcac gaggtaaata ttcaaatatt
      901 attttacctg cttgtagtgc tagaccgtca gctggttcag ttcttaacat agatacatat
      961 gcttctaatg catgtgttaa agcatcaaca cctgatgctg ctgttaatct tggtggcatc
     1021 tccatcatca tatcagcatc aattatagcc atgtttggca ttaattcata atctgctaat
     1081 gggtatttta cacctgaatc ttggtcagtt ataactgcaa atggagttac ttctgaacca
     1141 gttcctgctg aagttgggat agcagcaaaa tacgcttttt cacccatctt agggaataca
     1201 tatactcttt ttcttatatc cataaatctc atagctaagt cttggaaatc tacttctgga
     1261 tgctcataca ttacccacat tatcttacca gcatccattg cacttcctcc acctacagct
     1321 attattaaat ctggttggaa atcagccata gctttagctc cttctcttgc acaagctaat
     1381 gttgggtctg gagctacatc aaagaatgta gtatgcttaa ttcccatctc atctaataaa
     1441 tcagttaatt tcttagtata tccattatta tataggaaag aatctgttac tataaatgct
     1501 ttctttttat ccataacatc cttaaattct cttgctgcta cacctaaaca tccttttttg
     1561 aaataaacct tttctggtgc tctgaaccaa agcatattct ctctcctttc agctactgtc
     1621 ttgatattaa gcaaatgttt tactccaacg ttttcagata ctgagtttcc accccaagaa
     1681 ccacaaccaa gagtaagaga cggtgccaat ttaaagttat ataaatcacc tataccacct
     1741 tgtgatgatg gagtattaac taatactcta caagtcttca tagcatttac aaacttttct
     1801 actctctctg gatgatttat agagtctgca tataatgaag atgtatgacc atagcctcca
     1861 tctgcaacta atttttctgc tttagcaact gcatcttcaa aacttttaga cttgtacatt
     1921 gcaagtacag gagaaagttt ttcatgtgca aaaggttcat ttaattcaac agattctact
     1981 tcaccaatta ttattctagc tgtttttgga acttctacat ccgccatttt ggctattaca
     2041 tgtgctggtt gtccaactat tctggcattt aatgaaccat caattattac tacttttcct
     2101 actttttcag tttcttcttt atttaggaaa tgtgctccac gatatttaaa ttctttttta
     2161 acttcttcat atattttttc tggtactatt acagcttgct cggaagcaca tatcatacca
     2221 ttatcgaatg attttgatac taagattgaa cttacagcca tttttatatc tgcactctca
     2281 tctattattg caggtacatt tccaggccca actccgacag ctggtttacc tgaagagtat
     2341 gcagatttaa ccattccagg tccaccagta gctaatataa ggtctgcctc tgccatgaca
     2401 acattagtta attcaatcga aggaacatct acccaaccta taagtccttt tggtgctcct
     2461 gccttaactg ctgcatcatg tacaactcta gctgcttcta tagtacagtt tttagctctt
     2521 ggatgaggag agaaaattat accatttcta gtttttagtg ctattaggga tttgaatatt
     2581 gcagttgatg taggattagt tgttggtata actgctgata aaacacctac tggctcagca
     2641 attcttgtaa aaccaaatgc ttcatctttt tctatgacac cacaagtttt agtatcttta
     2701 taagcattat agatatattc tgaggcaaaa tggtttttta taaccttatc ttcaacaata
     2761 cccataccag tctcttccac tgccattttt gctagaggta ttctttgttg attagctgca
     2821 gttgaagcag ctaagaaaat cttatctacc tgttcttgcg taaatgttgc aaattctttt
     2881 tgtgccaatc taatttcttc taatctcatt cttaatgttt caacactgtt gacaatttca
     2941 ggactacata cttcaacatt tgatttttct actacctttt cttttttctc catactaaaa
     3001 acctccctaa aataattatg catctctaat ttttataaat cttaaatgca tcatctatat
     3061 ttttaactta tacttaaaat aattttatag atttcatctc tatatcttag attttattta
     3121 ttttactctc ttaggtcaag aagttttttt ctgaatattt tgtcaattta ttttttaaca
     3181 aatatattat tttttttact tttactaaaa ttttttatct aaatgagata tatttcattt
     3241 gaataattta cttcgactaa ataaaatact aaaatgataa tttatatctt tcattttttt
     3301 tacttattaa attttatatg ttatttgcat gttatttgta taagtagtaa ataaaatttt
     3361 tgtcgtattt ttataaaata acacttattt ttgacttgta tgcaattttt ctttttataa
     3421 caactgtata gttttaaaaa atatataaac taaaattatt ctaatctcta taagttattt
     3481 ttaagtcaat atgtaataat tatgcttttc atatagataa ataattttta tattaagacc
     3541 gttaattttt taatgataat atatatacaa aattttttac agtataaaat atttataaat
     3601 ttttagttta ttgacgtatt aattaatttt ttcccaggta gatttaatca atttatttta
     3661 aataaaagta aatttaattc gatatttgat tgggtaaaag tatatataat aataattttt
     3721 atattaaatt tgtagtattt atgtaagaaa taaattttat ataatatttt ttcactaaat
     3781 ttttttcttt ttacaacaaa tactcactac cttttgatat aatattttta tcaattatta
     3841 ttatattcaa gttttgaaag gagtattaaa atgagtttat tagttttcgg acataaaaat
     3901 ccagatacag attcaatatg ttctgcaatt tcattagcac atttaaagaa caaactagga
     3961 ataaaagcaa cagcttatgc tttaggagat atcagaaaag aagctaaata tgccttggat
     4021 tactttaagg tagatgcacc tgaaatttta gataatgtta gaatacaagt tagagattta
     4081 aattatgata aagtagtacc tttaacacca acttcatcta tattagaggc ttataacctt
     4141 atggatgaaa aaaatgttaa aacactacct gtaacttatg atgatggaac ttttgctggt
     4201 atcattacta tgaaagaaat agctaaaaat ctacttcatc aacactttac aactatacac
     4261 acatcactat caaacatttg taaaaacctt aatggtacta ttcttgtgga ctgtggtgaa
     4321 gatataaagg gtagagtagt cactttatca tttggaatgg agactctgat agagacttta
     4381 aaagaaggag acatagctat tgttggagat agatatgata gtattgaata tgctattgat
     4441 acaaaagtta aacttttaat acttactaat catacagaga tttcaaaaga tttgctagct
     4501 cttgctaaga taaatggagt gtctgttgtt tctgttgaaa gtgacaatta taaaacttct
     4561 aatgtaataa atcaatgtag tttcttagac tctatagaag ctacagaaaa tttaattaca
     4621 ttcaaagaag atgattatat ggatgaaata aaagagatta tgctagaaac aaacttcaga
     4681 tcttatccaa tattagatga tgataataag tttttaggtt tagtttcaaa aggtcatcta
     4741 ttaaacccat ctaaaaagaa tgttgtttta gttgaccata acgaatatgc tcaaagtgct
     4801 gatggaattg aacaagcaaa catagttgaa ataattgacc atcataaact tggcggtatt
     4861 tctactgatg ttcctatgtc atttagagtt atgcctgttg gatgcaatag tactataatt
     4921 tatcaaatgt acaaagaaaa taatgttgag ataccttatg aaatagctgg gcttctttta
     4981 tctgctatat tatcagatac attattattt aagtctccaa caacaactga tatggataag
     5041 aaagcttgtg aagaattaag taaaatagca aaagtagaca tggaaaaata cgctatggat
     5101 atgtttaaat gtggtacttc tttagatgaa tatactatag aagaaatagt aaatatggat
     5161 tttaaagaat ttaatatgag tgggcataga gttggtattg gacaagtatt taccttagac
     5221 atagactcta tatttgctaa aaaagatgag tttttatctt atataaattc gactgattat
     5281 gacaaactaa ttcttgcggt tacagatata atcaaagaag gttcttattt aatctataaa
     5341 gcagaagata gcttaattgc taacgcattt ggtgttgaag gtgttcaagg tacatttgca
     5401 ccaggtgttg tatcaagaaa aaaacaatta gtccctaatt taactactgc aatcaaaaat
     5461 tttaaataat ataaaatttt taaataacga aaactgtctc aaatctatat ttgagacagt
     5521 tttttattat atatatattg ataataaagt taaataaaaa tccataaatt taaaattttt
     5581 atgaatatat tttattatta ttgttatttt attcattcta aaataggtaa aactcaatat
     5641 ataaaaatac ttttgtcatt taaataaaac ctaaatagtt tttaagatta ttttagacac
     5701 gttttaagtc ataacagtat cagctattct aaatatcgtc aaagtagccg cagtttctgc
     5761 tgattgccca tttcgtatcc ctactgttgt agatgaaaaa ttatttattg taaaagtagt
     5821 cccaccaaat aaaaaaccag caaaatatcc agaaagtact cctccgcctc ctggctcagt
     5881 agtaaggcct gccaaatctt ttcctcctaa aattgggaat aggatagcga aagaatattg
     5941 atttatactt gatgaacctg gttgtaatgc tactgaaact gatatataat acacaccatt
     6001 ttcttgtact gtaaacgtac catttgcatc tactgttata cttgaactat ttcctattat
     6061 tgtttgccaa cctgttatag catctccatt atttagtgtt tgtggagatg gtgcatttac
     6121 gaggaagtta gcaactggct gagaggaatt gcctgttgct cctgttggac ctgttgggcc
     6181 tgttactcct gttgctcccg ttggtcctat acttcctgtc gctcctgttg gacctgttac
     6241 tcccgttggc cctatacttc ctgtcgctcc tgtatttcct gttggtcctg ttgctcccgt
     6301 tggtcctatt tctcctgtca ctcctgttgg gcctgttact cccgttggtc ctatttctcc
     6361 tgttactcct gttactcctg ttggtcctgt tgctcccgtt ggtcctattt ctcctgttac
     6421 tcctgtattt cctgttggtc ctgttgctcc cgttggtcct atttctcctg tcactcctgt
     6481 tgggcctgtt actcccgttg gtcctatttc tcctgtcact cctgtatttc ctgtcggtcc
     6541 tgttgctccc gttggtccta tacttcctgt cactcctgtt ggacctgttg gtcctatttc
     6601 tcctgttggc cctatttctc ctgtcactcc tgtcgctcct gttgggcctg ttactcccgt
     6661 tggtcctata cttcctgtca ctcctgttgg acctgttgct cctgttgggc ctgttactcc
     6721 tgttggccct atttctcctg tcactcctgt cgctcctgtt ggacctgttg ctcccgttga
     6781 tcctatactt cctgtcactc ctgttggacc tgttgctccc gttggtccta tttctcctgt
     6841 atttcctgtt gggcctgtta ctcccgttgg tcctatactt cctgtcactc ccgtatttcc
     6901 tgtcggacct gttactcctg ttggtcctat acttcctgtc gctcctgtac ttccggttgg
     6961 gcctgttgct cctgttggcc ctatttctcc tgtcactcct gtatttccgg ttgggcctgt
     7021 tactcctgtt ggtcctatgc ttcctgtcgc tcctgtactt ccggttgggc ctgttactcc
     7081 cgttggtcct gtacttcctg tcgctcctct tggtcccatt ggacctgtaa ttctaattgg
     7141 aagacactca catttaggat aacaattatc tataattcta tcttcaaatg ttacaagtat
     7201 atacggtttg ttacaacctc tactagatgt aaattcaaga atccgataag ggtaatttaa
     7261 agataaagct aacccataat taggatattt attcattgcc catgctttta tagttcccgt
     7321 aatatttaaa caaatatagt tggatgtatc acttatccca attggtaaat atcctctgat
     7381 attttccata gacactctat cattccatgt tatagttcgt aaattataat attcacttat
     7441 tcttttaatt tcaatatcta ctttttcttt aaaaatatga ttcttatttg aatcaatatg
     7501 aataaataac tctgctttta atatactttt gtcttttaat atatctggta ttctcacttt
     7561 aaaaaaagta atcaactttt cttttataaa actatttgaa catttacttc ctattaagca
     7621 ataatctaca ttactaaagt ttttatctgg atatttttta gatataaaag tatcatcatt
     7681 taaataaagt ataatatttc tcatttataa tccctccatt ttgggtctat attatactat
     7741 gtttaaaata catacttagt tacaacacaa ttaaaattta cagtcttaaa tttttatata
     7801 ataatactga tttctacaaa aaaagaacat gtcattagaa acttctaata gcatgttctt
     7861 tttcattatt atatattaca ctttaaatta ttccttgact ggcttagctg gtgtaaacta
     7921 aaccagtctt tctaaaatat taagcacttt tttcttatta tgttttttag gatttattgt
     7981 ctctattatt gtaaaccaaa gttataaaat tgtaaataga atatattacc ataacaatat
     8041 ttttttataa gtaaaaatta taaaaaacct tatttttatt aaatagaata aataaaataa
     8101 gtttaaaaaa tactgaaata agtgttttcc acatatctta tatatttatt taatacatta
     8161 ttatttaaaa ataaatatat aaacaatcat aataaaattt ttttaataaa actttatttt
     8221 ttagttaaaa tcatacaata tatagttttc cacaatacaa ataaattaaa aagtgaaatg
     8281 tatttgtcta aaaacatact aacatttcac tttatcttca ttattaagta atatatatct
     8341 ttaattttta tttataactc aacctagttt ttatataagt tttagttttt ttctacatct
     8401 gagtaatcta ttccaaaagt ttctacagta acagacttca taatttgtgg agtttgtggc
     8461 ttatcagctg aatctgttct aactgatgct atttcatcaa ctgtatccat accttctatt
     8521 actctaccaa aacctgcata ctgaccatct aagtgtggtg aatttttatg cattatgaaa
     8581 aattgagaac cagctgaatt tggtgccata gttctagcca ttgatatgac tcctctttca
     8641 tgttttaaat tgttagtaaa tccattacca ctaaattcac cttttattga gtgacctgga
     8701 ccacccattc ctgtaccatt tgggcatcct ccttgtatca taaatcctgg tattactcta
     8761 tgaaatatta ttccattgta atatccttca tttactaatg atacaaagtt tttaactgta
     8821 tttggagcta tatttggata taactcaatt ttgatttctt ttccattttc catttctata
     8881 gttactatag gatttttatt ttccataatt tattaccttc cttttcatta gttaaattac
     8941 ataattacat ttattatatt atactctaaa ttaaagtcta tcaagtatat tttttcctat
     9001 ttcgtatcct tcttgataaa tagttttaca aaacttatca tcaaagttaa gcgtatcttt
     9061 aggagcaaat tgagtattag gtctaactac tattatttta tcttcatcat atattttttt
     9121 aatatcctct ggcaactcat aattatattt tgtggatact aaaataattt tatccacatt
     9181 atccccaatt aatggttcaa ttaatgcact cctaattaaa cctccatcta atttgaaacc
     9241 atcataaatt ccttcttgca catctttcac aaattgttct ttaaatccaa gagttgcgtt
     9301 tagatttgat ggtaacaacc cactgtatga aatgctgata atagcttcat tacgaggcat
     9361 ttttgatacg tcaacaatct tttctgatac tactttatta tcaacttcaa cataattaac
     9421 ataaaagttc atttcttcta tattactatc atctagttcc cttaggttgt ctatagcagg
     9481 agaattatcc acagtattat ttactatttt tacatttcca ttctggatat cttttatatt
     9541 tgtccacatt ttctccaagt tatcaacatt ccctgtgtac aaataatatc catttattgc
     9601 tcctattgat gtccctgaaa tcgaataaaa tttatttatt cctccatcat acaaggcttt
     9661 aactactcct gcttgataag ccccttttgc tcctccacct tctaaacata atcctaattt
     9721 catagtctca tctccaaatt gtttatctta tatttatatt tcaaaaaact tactaaatgt
     9781 tttatatata ctatagtggt cctcaacatt tcctaattct cttatagagt gcattgctaa
     9841 aattggactt cccacatcta cagatggtat atctatatga gtagaagata ttggacctat
     9901 agtacttcct cctctttcat ctgacctatt aacaaattct tgatactcaa cattagcttc
     9961 tctacatata tttttataaa tagctataga gtatccatca ctagtatatg cctgactagc
    10021 atttatcttt ataaccggac cttttcccat tattggttta ttagttgggt catgcttttc
    10081 tggaatgttt ggatgaatag catgagctaa atctgatgat atcataaaag atgaataaat
    10141 agactcaaag aatccctctc tatccttacc cattgatata caaattctct ccaatatatt
    10201 taacaacatg tttgaatctg caccttgttt agtagagcta ccaacttctt cattgtcaaa
    10261 tacagccatt acatttacac catgttgacc tttagtatta acaagagcat tcaaactagc
    10321 atgtaccatt gcaagattat cttgtctccc aatagatata aattcatcat tagcccctat
    10381 taaacttcct ttttcaaatt catataaaaa taaatcaaaa tctataatat cttccatatt
    10441 tacatctaag ttagatgcta tttgctttat aagaaaatta tctttttcta atgtatcatt
    10501 taacatccca actaaaggta acatatcttt ttgcttatta tatgagtgac catcatttat
    10561 gcttctattt aaatgtattg ctagatttgg tattatacaa attggctttt taaagtctat
    10621 aagcctttca tttggtttta atatactatc accttttaaa actactctac cagctatccc
    10681 aagaggtcta tccaaccatg tacttaatat tgctcctcca tagcattctg tatttaactt
    10741 caaataagat ttttcagctt ctatttctgc atttggttta atactaaatg taggagaatc
    10801 tgaatgagag cctattattc taaatccatc tctttctata tcttctgaat ttactacaaa
    10861 agctactata gctgataaat tcttagtaac ataatacttt ccaccaactc ttagattcca
    10921 tctttgattc aaaactaatt cttcaaatcc atttttatct aagatttcct tagtactctc
    10981 gactgcatga aaagacgacg gactatcata tatgaagtct attaagtttt tagcaaagtt
    11041 tatactatcc attcctcatt tacctccatt ataaaatatt tagtgttgta tattctttat
    11101 ttattttttt aattccctgt tggtcaaaag ctatagtaac atctgtacca attatagaaa
    11161 ccactgtccc aaccccaaat ttaggatgat gtaccttaga accaagtttt atatcatctg
    11221 gattagtttc tttattagaa tcttttattg tagcattaac tttatctgca actttagatt
    11281 tgcttatagt actcatatac tttttagtat atttatctaa catattataa cttgctttag
    11341 aatagcttag ttcttttttt actttgtaca atctttctat acattcttct ggcagttctt
    11401 ccataaatct tgaagctatt gcaacatttg ttttaccata taatgttctc ttctctgtca
    11461 atgacatata tagctcttct ttagctctag ttataccaac ataacaaagt cttctctctt
    11521 cttctatttg tgaatcactc atagatttta tagctcttga tataggaaat aacccttctt
    11581 ccatacctgt taaaaacaca acagggaact ctaaaccttt ggatgtatga attgtcataa
    11641 gcgaaaccct atcattgtct tcttcatcat ttgattcaga tgtaagtgct atacttgtta
    11701 ggaatgtctc taagtcttta ttttcataag tatcatcatt actactttca aactccaaag
    11761 ctatagaaat aaattctctc aaattatcaa ttctgtcttg agcttcttca cctttgcctg
    11821 ttaaatcttt ttcattttta tttcttatct ctacaagctc gtccatatat cctgtagtat
    11881 ccaagacttt ttctataagt ttgctaacag gataaacttc ttttatagtt ctaagagtcc
    11941 ctataatatc aacaaatcca cttatacttg cccttgcctt agtagatata tctgaatttg
    12001 tttctatatc aattaatact gagtatatac tttcctgttt taagttagct ctatcttcaa
    12061 ttttttctat agttctaagc cctatacctc ttctaggaac atttataatc ctttttatag
    12121 atatatcatc ttgtggattt tgaataactc ttaaatacgc caacaaatcc tttatttctt
    12181 ttctctcata gaattttgta ccaccatata tattgtatgg aatttgactt ctatttaaag
    12241 cgtcctctac aggtcttgcc tgtgcatttg ctctataaag tactgcaaaa tccttataag
    12301 gtctattttc ttttctagct atttgtgcaa ttgaatcagc aatgaaatca gcctcttcta
    12361 tttcgctacc agttaattgt atctttataa gttctccttc ttttttttca ctccaaagct
    12421 tttttctttt tctttctata ttattagata taactttatt agctgcatct aatataactt
    12481 gtgtagacct ataattttgt tctaacttta caacatgtac attgtcataa tctttctcaa
    12541 attcaagtat gtttcttata tctgcacctc tccatccata aatactttgg tcatcatctc
    12601 caacaacaca aatattttga tgttctcttg ctaataattt tatcaattca tattgagctt
    12661 tacttgtatc ttggtactca tctaccatta tgtatctaaa tctacttctg taatatgcta
    12721 acacttcatc attagacttg aaaagttcta cagttttaag tattaaatca tcaaagtcaa
    12781 gtgcactatt tctttttaaa cggtcttgat atagcgcata aatatctgca atcttaacca
    12841 ttctattatc tgccatattg atatctttaa actgctttgg agtataaagt ttgtcttttg
    12901 ctcctgaaat tgcacttata actgcttttg gttcaaacac tttatcactt aaatttaatt
    12961 cttttagaca gtctttaacc aatgtaactt ggtctgctga atcataaatt acaaaacttc
    13021 tattgtaacc aatcttattt atatcttttc ttagtatacg aacacagcaa gaatggaatg
    13081 tacttatcca catatcctta gtttcaggac ctatattttg ttcaactctc tctctcattt
    13141 catttgcagc tttgtttgta aatgttatag ccaatatatt tggtgcttgt acacctttat
    13201 cttctataag atatgctatt cttgttgtta ataccttagt tttacctgaa ccagcaccag
    13261 ccagtataag tactggacct tcagtctttt ctactgcttc tctttgagca ggatttaatg
    13321 tatctaaatt catatttctc ctcctgttaa cagccataaa tatattttaa tctaaaatat
    13381 gaattacttc aaattataat tatgtattat attaatagat tatttccaat atattacaat
    13441 taaataacat ttttccatat aatttatttc attgtttaat aaaaaaaata accttatgat
    13501 ttggggaatc ataaggcacc tttaaggggt ctaggttaag ggttattata tataaatcta
    13561 tttttttaaa ataggtttag ggcttaatta tttatttgta ttaagttctc tttccttcca
    13621 atacttatac aaatcttctt ctttcttaaa taagggtaaa ttcatatttc tatgtacttt
    13681 tactaaccat ggggattcta gaccagcttg tataatatta ttttcattta taaacacatt
    13741 ttctgctttt ccttcatcaa tcaaaatccc cttatccaat acatatatgt aatcacatat
    13801 ttcatacatt aaattcatat catgactgga tattactatt tttatattat ttttatttaa
    13861 tccctttatt atatctacaa tagacctagt acttactggg tctaaaccag ctgtaggttc
    13921 atcaagtaat acaatttcat tttccattgc tataactgat gctattgcca ctcttttttt
    13981 ctgtccatag ctaagaaaat gtactggcct gtctataaaa tctattccat taacagctac
    14041 tagagctttg tttattctct ctttaattgt tttttcatcc ataccaatgt ttctcattgc
    14101 aaaggctata tcatcatata cttttgaata aaaaatttgt ttttctgggt cttggaagac
    14161 aatacctaca ttttttctaa ggttgtataa acttctctta tcatatttta acttttcttc
    14221 tttaaaaaga atttctccag atgtaggctt taatataccc attaaattca taaataatgt
    14281 tgattttcct gaaccatttg aacctattat acctataaca tttccctttg aaaaatccat
    14341 attaatgttt aatagtgcat tggtattttt ttcatattga tacgttaaat tatttatttt
    14401 aaacatcatt atcccctact atgtgaaatt taccatcata caattttatg tctaatgata
    14461 tacacatatc atcatacctt gtcataagtc ttttgtacag catacatcca agaataccta
    14521 aggatttata tgaagttttt aaatttatat atccaaatct tagctcttgt gacttgtata
    14581 tttctgaaaa ttcttctaaa aatatgaata taaacctata tattaacatt gataactcga
    14641 caaaagtgtc aggtaaatga agttttttaa aaaagaatat taattgatta aaaggtgttg
    14701 taagtataca aaaatacaca caagtcaaac aagacatcgc tctaaataac acatgaatag
    14761 acatgtcaat agacgccttt gatattccta cattaaaact aaaaaattca aaactatata
    14821 ataaatctgc tttattaaaa gatatattta caagagttat tcctatacta agaaataaaa
    14881 agtacattgg tattctaagt aattttaaat aattttttaa atctattcca gccacacaaa
    14941 caaccaaaat actcattaaa atcataattc cacttaaaat aaatatattt tgtattacca
    15001 tagaagcaat cagaaaaatt actcctatgg ctactttttt gtttggattt actttactca
    15061 atctatttgt atatgcatat ttatcaatta taagcatgat taacttctct ttcctttttg
    15121 aagacctaat atgtatccta ttacaccagc tcctaaagct gcttgtgcag agaataataa
    15181 actttctatt tctccacttg gtggctcata tggactgcta aaccatggtt tgtaatttgg
    15241 atttatctta gtaatttcac tttcagcttg accatctgct cctccatatt cagcacctga
    15301 gttgacaagt aatgggaata taataagagc tactactaat aaaagtaata atatgttagt
    15361 ttttgttttt gttttagcac tcattagctt taactccttt atcattattt tctagtatgc
    15421 ttttttctcc gttttctgta attaaattat atacaattac agtaagaata ccctctgcta
    15481 ttgctattgg tatttgagtc ataaagaaaa ttcctaaaaa tttaattgct gaagccatta
    15541 ccccacctga tgggtctggg aaagccagtg ctaattgtat tgaagttatt gtatatgtgg
    15601 ctaagtctcc tattgctgct gcaagaaata ctggcattgc attatttcca tcttttttct
    15661 ttaaagcctt aaatattagg aatgatacga ttggcccgat tatcgccatt gaaaaagcat
    15721 tagctcctaa agtagttatt cctccatgtg ctaataacaa tgcttggaat attaaaacta
    15781 tagttccaag aacaaacatt acgcttggtc caaacattat tgctccaaga cctacaccag
    15841 taggatgtga acaacttcct gttactgaag gtatttttag tgctgataat acaaacacaa
    15901 atgctccaca taaagctaat aatactttct ttttagggtc ttgctttact attttaccta
    15961 ttgattttaa tccgaataag aaaaatggta taaatataac tccccatgct atagaccatt
    16021 ttactggtaa gtaaccttcc attatatgca ttgcatttga tgtaactgga gttaaaatga
    16081 ttaacataag tgcagctata acccctaatt taatattctg tttcatctta gtatccctct
    16141 ctccctttct aataattaaa acataataat acatgtctga tttatttaat aaaataaaaa
    16201 agcctaactc gatggttagg cttctaattt tccacaatct aaatacaaac acataaaaca
    16261 aacttatctg tataattatt ataagaaact ttctccatcc ccgaagaaat tactttaata
    16321 ttttaggcag gtctcctgac ttagcttcat cctactcctc ttccttccca tgagatataa
    16381 aaaatatcat catagtggct aacaaaagtt tcgtctgcat cacagtagcg ggggctgtaa
    16441 aggaatttaa cctttttccc tattaatctt gcttaaagaa cctataatat tatatattta
    16501 atttttaaat tattcttaaa tcattttcta tataaatcat tgtatacctt ttctgtgaaa
    16561 atttctacat tttttataaa ttttattcat ttttatattt ttatgtatat aaattctttt
    16621 ttgtaagttt attaatttct aattatcttc tttaaataca ttgaaactac atcagaaaat
    16681 ctagttatat ctcttgaata tataaaatca tttccatata ttaatctttc agcttcaagt
    16741 tctgattctt ttcctgtaaa tactcctaat actaatatat tttgctttct agcttttctc
    16801 acttcattgg ctgtatctct caaaccaact atacccctat aagccttttc tcctcttaat
    16861 gttctctcac tactttttcc tatctttaca tcatttggtc ttccatcgct gagaactatc
    16921 aggattttat tttcttcatc tctatttatt agaccactac atatagattt tattgccaaa
    16981 ccatctctat tattaccagc acaaaaatat tcaaatatat tttcactatt ttttatacta
    17041 tcatcataat ctttaaatct ttttaatatt gtatagtcta aaaaactaga aaatcccatc
    17101 actctatttg gtatgccaac ttctgttaga gcatttgcaa ttatgtaagc ttgaattgcc
    17161 acatttccct gatttctact ttgagagcca cttgcatcca ataatatatc aataacgtat
    17221 ttcccttttt cattttctat atctttataa aatattttat tattattact tctaccaact
    17281 ctccaagctc tatttgctaa tatagtacca caatcagaat atactctact tttagcattt
    17341 tcttctatta aaattcttga cagactttct tttaatctct ttatttgctg tctatacatt
    17401 ctagcatgtt ctctaaattt tcctaaatta ttttcttttt gtcttgttac atattttagt
    17461 tgaaatacat tattacactc acttctaaga actccatctg taaagtgtat tctacaacct
    17521 tcatgaacat ttctacaatt ttgtgtctca atttttttaa tttcaccttt actcaaataa
    17581 gtttttccat aataatgttc tatttttgaa tatatcttat ttgcagtgtc ttcatcaaca
    17641 tatataactc ggtctgctga attgctacat ttagaatcat caaaatcccc tacaccttct
    17701 acaagcatgg atgaagatat tgaatctata tcactttcaa taatattttc ttcttcatca
    17761 ctataaagtt cttcatacat gaaatcagca aatgtatcaa aatttacttc tacactttct
    17821 acattatttt catcaataag actattttct tctccattta atatgtattc aaaataagtt
    17881 aaatatataa catctaatgc ttttaaaatc tccataatat cttgataggt atctatattt
    17941 cttatatcat taactattct tctagtcata ctatctaatg ctgggtgctt gtccatacta
    18001 taaagtacaa atgtgtattt aattttatcc ataatattat cattgtgtat tttggtaaaa
    18061 cgtgaaacaa tatctttata agcatttctt cttatgtctt ctatccctgg cctatcttta
    18121 atgacctttt cttcaactat tgtatttaat ataacttgaa ttagacccag tataatatct
    18181 ttgttgtatc cttttcttat tctatttgta agatattttt taactatatc ccaatctaca
    18241 tactttcttc tagcacctgc aattattgca aaatatagac ctatttcttt tgaagagtaa
    18301 tctttttctg atatatctat actttcattg taatcaccag ctattgtcca tgataggtta
    18361 ttagctctat tttcaaattc ataatcatca tatatccatt tcataatctc acctatatta
    18421 aatatgactt attggaaaca catctatact ctcccattta ttaggtattc tagttttaat
    18481 tacatctcca accatttctt tctcatatac atcaaaagtc tttcctgtaa gtcccatatt
    18541 aattgcaaga gttggtttta atcctcttct tatagtctta agagatgcca taagaccacg
    18601 taaatcaata gctttactag atatctctcc attttgagac ttcaattgta aatctaaaaa
    18661 tattcctgca aagtgtatta atttttcttc atcagcatct gaaaattcat tttttaaaat
    18721 catcattaac ttatcttctt ctataggagg aatatctatt accataaatc tagataccag
    18781 tgcttcatta agttcttttg ttcctgcata ttcataattc atagtcccaa taaatcttgt
    18841 tgctggatgt agattgactc tctcataccc aggtacatca attattcttc tatagtctaa
    18901 agctgaatgt accacaacta tagcatcatt ttttgccata ttaatttcat caaatacccc
    18961 aaaacctcca ttaatagcac actcataaac tgagcctctt ctaagtttaa cttcattgtc
    19021 tataaatgta tctgtaccta taagtgttga actgtctgta tttatatgaa atgatgtatt
    19081 ccactgaggt ctattaaata tttcccctag attatcagca agtaagtttt ttcctgttgc
    19141 ctttggtccc gataataata tattttcttc ttctaaaata gctgctatac acatagacaa
    19201 gatatcttta ccataaaaat aagttttaga ctttgttact ctgtattcta aatctttttc
    19261 taatccataa taatttctaa aatacagtac atcttcaata agtttttcat ctaccccttg
    19321 cacccttaga ttatctatta tatccatatt tttacccctt ttatataaat agtatttgtt
    19381 tcatttctta tcaccatttt agcataaaag gatataagct aaaattgatt ttttaatctc
    19441 cccattttaa gtaaaaccat gcaccatttt atcactacaa aattaagagt actacttact
    19501 gatatttctt gattttgttc gtctatacgt atataatata tgctgataaa ttatgaggaa
    19561 aggatgattg aaatatggaa tatatgtctt gcccagaagt agcaaaaaaa tggggtattt
    19621 ctgaaagaag agtacaaaaa ctatgtgaag aaagtcgcat accaggagtt tcaaaactcg
    19681 ggtatatgtg gctgatacca aagaacgcag aaaagcctgt tgatggtaga acaaaaagaa
    19741 gaaaggaagt acaatatgaa tagtgttttg attatagatg atgacaaaga actttgtgct
    19801 cttatgaaaa aatgtgttga gcaagaaaat ttatctgcta ttactgcaca ttgtggtgca
    19861 gaaggtctac atttggtcga taaaaataga cacacactct ctcttgtaat tttggacata
    19921 atgatgccag atatagacgg ttttcaagtt ctgaaaaaaa ttagagaaac aagtaatatt
    19981 ccagtaatta tgcttactgc taaaaatgac gaggatgata aagtttccgg actacgttta
    20041 ggtgctgacg attacctaac aaaaccgttc agcataaacg aacttatggc tcgtgtcaat
    20101 tctcttattc gtcggtatac tatgttaaac cctgctttta taacagacac agattgcata
    20161 ataataaatg gcatgattat tgacaagaca aaccgaattg tttctgtcaa taatattcct
    20221 gttcaactta caagcaaaga atttgattta cttgcttttc tggcttctaa taagggacgg
    20281 gtgtttacca aaaaacagat ttatacacaa gtatgggaag aagaatatgc ttttgacgat
    20341 agtaacatca tgtctttcat cagtaaattg agaaaaaaga ttgaaccaga ccccaaccat
    20401 ccattttata ttttgacagt tcgtggtgtc ggttatcgtt ttaataagga ggcttaacat
    20461 gaatacaagt gtctatctgc ttttgatatt ggctcttagc ctgattatta tagcgtacct
    20521 tgtttctgtc ctgctacgtg tccgaataca acttacactt ataaaagatg ctttagagga
    20581 tataaaaaat ggaaacctta atcgccgtat tttaacagat gaaaacgaca ttacaaaaca
    20641 aatttgctat gatattaacg aaatagcaat caatagccaa tttcaactta ttcagcaaaa
    20701 acaatcagaa caagcctaca aacagctaat gacaagtctc tcccatgatg taaaaactcc
    20761 acttgcttct ttggtcggtt atttggaagc agtagaaagt aaaattgttg taggagagga
    20821 aaaagacgag tatatccatg ttgcgtctaa taaggctcat tacttaaaga attttgtaga
    20881 aaatctattt gaatgggtaa aattggattc taaggaacaa gtttttcatt ttgatatttt
    20941 tgacttgaat gaattatccc gtaatattat atctgattgg atacctgttt tagaaagcag
    21001 tcattttgaa tatgaatttg atataccaga aatagagtac ttcttacgta tagacgcaaa
    21061 tgcatatacc cgtatcatta ataacctatt acaaaatatt attacccata gtagtggcaa
    21121 caaaatgaca ttgagaattt ttgaaaacaa agagcaagct caaattataa taacagataa
    21181 tgggaaaggt atatcttcag acaatctccc acatattttt gaaaggttgt accaatgcga
    21241 ccattcacga gcttcaaagg gaaatggttt aggcttggcg attgcaaagg aacttattaa
    21301 cgctcataag ggaaatatta ccgccaacag cactcccggt atgggaacag aatttacaat
    21361 attgcttcct aaagccctgt aaaagtacga ggctttctta ctctaaaaca agataaaagc
    21421 aagattatgg caaggttttg gcaaggttca tgttttataa ttctaattgt agttgatttt
    21481 gacgttgtat caactatctt ttgtagaaac aaatttatta aaagcgagga aattatatat
    21541 gaatgatttt gtgattgaaa caaagcaact tacaaaaatt tatgggaatc aaacagtagt
    21601 tgacaaagtc aatctccatg taaaaaaagg ccgaatatat ggtctgttag gacgcaacgg
    21661 agctgggaaa accacaatta tgaaaatgat tttaggattg acttcaatca cgtatggcga
    21721 ggtagatgta tttggagaga atattaaggg acgtgaaaaa cgagtatatc cccgtatcgg
    21781 tgcaattatc gaaacgccag gattttatcc taatctgaca ggaacggaga acttggagat
    21841 ttttgcaaaa cttcgtggta cagccgcccc taattctgta aaaagagcat tggagattgt
    21901 aggcttacct tataaaaata aaaagctgtt tagtaaatat tcacttggca tgaaacagcg
    21961 acttggtatt gccaacgcta tcctgcatga cccggaactg ttagttttag atgagccaac
    22021 aaatggtctt gaccctattg gtattgcgga gatgagaaag tttattaggg acttgagcgt
    22081 tgagcatgga aagacaattc ttatttccag tcatattctt tcagaaatct cattgttggc
    22141 tgatgatatt ggaattatcg accacggtgt tcttttagaa gaaagtagta tgagtgaact
    22201 agaaaagaaa aacagtaaat atatcctgct acaagtttca gatattccga agacatcact
    22261 tattttagag cgtcaattta acacgaagga ttattccgtt caagatgacc acactttaca
    22321 cctttataac actactctgg atatgggtga aatcaataaa gcacttgtaa tgcagaatgt
    22381 aactgttatt agttctcaac tttgtaatga tactttagag gactacttta aaaaaatcac
    22441 aggaggagag ggtattgctt aaacttattc aaacagaatt tcttaaactg cgtcgtagga
    22501 aacttatttg gctgatgttg ttgtctgctc tcattatgcc gtttttcgca ctactatact
    22561 tcaattattt tggtaaaaca ggtgttgagc caacaaagtt ttataaatgg tctgcatttg
    22621 gttatacact ttggattatt ttgcctgttg ttttgggtat attttgcacc atgcttatac
    22681 acgaagaaaa ccagtatgat atgctcaaac agctttggat tgtacctatt agtaaaatgg
    22741 gatacttttt tagtaaattt ttcgttgttt tgatttactc tatttgtttt atgctaatta
    22801 cagctgttgc ttctgtgtta tttagtgtac tttctggata tgtcgtattt agctgggcaa
    22861 gcattttata tttgttaaaa aaatgtttag aaattggtgt aatcactgct tttgtaatgt
    22921 tgccgatttt ggcaatcgct acatctcaaa aaggatacat tctcccaatt tgtatcacat
    22981 tggtatatgc cttttccggt tttgttataa tgacaataaa tatgtattta cacccattat
    23041 ccagtatggg agctattatc atgagaaata aagacattcc aggtcttata ttttcacagg
    23101 aaattaatgt cccattagca tttctttgta tttgtatttg gagtattgta tcagtattgt
    23161 ttgcaaacat tgcactagga agaagaaagt gaggtgtaga taatgtggaa attactttgg
    23221 gctgaatgtc aaaaaattcg tcgttcaaaa attgtttgga ttacagtttt tgcatccatt
    23281 atgatagcag tcatagtttt tgcaggaggg caggatgtat ataatgaccc agatttacat
    23341 tatggcttaa agagcgttta tgacggttca cggtatatag acaatacagg ttggtatatg
    23401 gatgcggtac aaccttggtc aacttttttt gttcttcctg ctgtcattgc cctattagga
    23461 ggctatatta tttgtcgtga agaagaggag gacactatta aatccctgcg tcttattccc
    23521 ataaatgaaa caaaattaac catagcaaaa atgattatta cttttatatt cagtatactt
    23581 ctttatttgt tactctttac tataacattt ttgactgaat ccgttttaca tttttccgat
    23641 ttatcgacaa aactggtttt aagttgcatg aaagagtatt ttcttgatgg cataggtgta
    23701 ttctttgcaa tttctcccat tattgccttg gtatcgcgca tgaaaaaagg atattggctt
    23761 gcattagtat tcacagaagt ttattctatt gctggactat ttgcgggtat gtcaaatgtc
    23821 ttacaaacct tttttcctat tgcggcaata tttaatattt ctggatacta tataactact
    23881 ggagaaaaaa ttatggggag tattataagt ttattacttt gtgcctgcct ttctgcattt
    23941 atactgaaag ggctaaaaca caatgaaaaa ttgaaatagg tatatcaaaa tgaaaaagat
    24001 aaaaaaactg attattctaa ttgtattctt gtttcttttt ggcatgttac actgttatac
    24061 tgaatggaaa ttcaacaatg acccacctaa aggggtaata gaaagaataa atgagttgac
    24121 tgataagatt ggactggatt agtaacttat gttaattata gtgaatgtaa tattagcaag
    24181 ttattgtttc gtattttaat actataactt taaatatttg gttttaatct tccaaacgaa
    24241 tataaaaaaa ctgtcacaga ggcaacatat ttttgaatat ttataaagtg gtagttttga
    24301 ttttcaaagc tgccgccttt tcttctatat aagcaaactt atcctcaaca aagtgtaaaa
    24361 catggtacat tttaagaaaa aagtaccaca gacacaagca ataaaagaaa cgccgagaac
    24421 ctacaaaaac gtagtgctta tgcagattta cggcgccttc tattttctca accgtcttaa
    24481 tgaccttgta caaaagaaat ttttctaata ttttttataa tcttatcttg tgatgttttt
    24541 tatgtataaa tgacttaata acaaatccaa taaaaattat ggatattggt ataattagaa
    24601 cgaggaattt ataatcatta taatgtgaca ttattcttac ccaactctca gaaaaaatat
    24661 atcctatact gattaatgaa gtattccata tggttattcc cacgatagat gatattacaa
    24721 aagttataat attttgacct gctatacctg ctataaaaga tatgtatgtt ctacataaag
    24781 ggattaatct acatacacaa acagaaatag aagagtattt attaaaataa tcttgagatg
    24841 caaatattcc tttctttgcc tttggacata tctctattat tttatttatg atagatttac
    24901 ctccccaatt tcccactgta taacaaaata tcgaaccaag tatacctgca attatactta
    24961 aaattaaaat tgtcagaaat ccaaaatgat ttcttgcagc aattgctcca cataaaggta
    25021 aaacaacttc gctaggtaat ggaaaacaag catactcaat cataattaat acaaatacag
    25081 agattaatcc atattgtaaa ataaaactat tgattacaga cacttactta tctatctcct
    25141 ttcttattta aaggttttaa gggtacatgt tctaaggatt ctcaccctat atttaaagaa
    25201 tcaatctctt taaactatta tatgtattta cacaaagcat ttataacata ctttaaaaat
    25261 agtaccttaa aataagaaac ttaataaata aaaaaagatt acgtggctac gtcctactct
    25321 cccaggcagt ttcccaccaa gtaccatcag cgctagagag cttaacttct gtgttcggaa
    25381 tgggaacagg tgtatcctct ctgctattgt aaccacataa tctttatgct taatatttct
    25441 attaagtttt taataagttt atagaa
//
LOCUS       DAEMHW010000041        25403 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEMHW010000041 DAEMHW010000000
VERSION     DAEMHW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25403
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.041"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            475..852
                     /locus_tag="KPK67_001440"
     CDS             475..852
                     /locus_tag="KPK67_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2458432.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            845..1867
                     /locus_tag="KPK67_001441"
     CDS             845..1867
                     /locus_tag="KPK67_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG2458433.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYSYSKSELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNRVNLDCELCCDESVIYNCSFNDKKEYALALIKSIKLSKECNRGILLTDFNRTNLE
                     KRLESIISKKFKNSVFVVIILCLLSSITYFKFDIFISKPVIYSLPKPSVLNDEYIYNT
                     DIRVER"
     gene            1929..2522
                     /locus_tag="KPK67_001442"
     CDS             1929..2522
                     /locus_tag="KPK67_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG2458434.1"
                     /translation="MLKKIKKVLSIACCLFILGGSISFADLENNNEINDKSVENPIYE
                     FNIQGYTFTYENAPQRIKDEYNKTCRELNITPSPSTEIFIPIDEISKYTDTVPLFEID
                     EFNVKYFGTRFEVTGAKTYTVGVSTYVGYGHTTSGNPVHLAQLLLKKSGYSIAIDSLF
                     GSSTYSKVRSFQSKYGLSVDGVVGLGTWNKLAQVTNA"
     gene            complement(3317..4333)
                     /locus_tag="KPK67_001443"
     CDS             complement(3317..4333)
                     /locus_tag="KPK67_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG2458435.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWN"
     gene            complement(4662..4844)
                     /locus_tag="KPK67_001444"
     CDS             complement(4662..4844)
                     /locus_tag="KPK67_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458436.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            5157..5576
                     /locus_tag="KPK67_001445"
     CDS             5157..5576
                     /locus_tag="KPK67_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBG2458437.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRTNKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            5707..7548
                     /locus_tag="KPK67_001446"
     CDS             5707..7548
                     /locus_tag="KPK67_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2458438.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLCGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYKNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFTSLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            7575..9461
                     /locus_tag="KPK67_001447"
     CDS             7575..9461
                     /locus_tag="KPK67_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2458439.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKANNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            9469..11469
                     /locus_tag="KPK67_001448"
     CDS             9469..11469
                     /locus_tag="KPK67_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2458440.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            11731..12990
                     /locus_tag="KPK67_001449"
     CDS             11731..12990
                     /locus_tag="KPK67_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2458441.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            13051..14838
                     /gene="iorA"
                     /locus_tag="KPK67_001450"
     CDS             13051..14838
                     /gene="iorA"
                     /locus_tag="KPK67_001450"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBG2458442.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDIPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            14840..15415
                     /locus_tag="KPK67_001451"
     CDS             14840..15415
                     /locus_tag="KPK67_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBG2458443.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            15468..16538
                     /gene="buk"
                     /locus_tag="KPK67_001452"
     CDS             15468..16538
                     /gene="buk"
                     /locus_tag="KPK67_001452"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG2458444.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            16756..17457
                     /locus_tag="KPK67_001453"
     CDS             16756..17457
                     /locus_tag="KPK67_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458445.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHANN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            17517..18023
                     /locus_tag="KPK67_001454"
     CDS             17517..18023
                     /locus_tag="KPK67_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBG2458446.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            18198..19220
                     /locus_tag="KPK67_001455"
     CDS             18198..19220
                     /locus_tag="KPK67_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG2458447.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(19367..19675)
                     /locus_tag="KPK67_001456"
     CDS             complement(19367..19675)
                     /locus_tag="KPK67_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG2458448.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(19735..20163)
                     /locus_tag="KPK67_001457"
     CDS             complement(19735..20163)
                     /locus_tag="KPK67_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458449.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(20386..21843)
                     /locus_tag="KPK67_001458"
     CDS             complement(20386..21843)
                     /locus_tag="KPK67_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG2458450.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTIRSGRKLKSKADTKLAD"
     gene            22261..23175
                     /gene="nadA"
                     /locus_tag="KPK67_001459"
     CDS             22261..23175
                     /gene="nadA"
                     /locus_tag="KPK67_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBG2458451.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            23188..24489
                     /locus_tag="KPK67_001460"
     CDS             23188..24489
                     /locus_tag="KPK67_001460"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBG2458452.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            24470..25306
                     /gene="nadC"
                     /locus_tag="KPK67_001461"
     CDS             24470..25306
                     /gene="nadC"
                     /locus_tag="KPK67_001461"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBG2458453.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
BASE COUNT        10013 a         2683 c         4235 g         8472 t
ORIGIN      
        1 ttagtagata ttaaaagcac attttttata aaagtgcttt tttataattt atgaaaggta
       61 taattataaa agtcttattc aataaaattt ataattaatt tagatatatt tgtaaataat
      121 cagtataaaa tagatgtttt aatatcataa aggcataatt agaaggttat taaaatttat
      181 tagatattac tctagtttat tttaaaaaaa agtattataa tattccttgt gtgaaaatat
      241 aggaattttt attaaatagt tttaatgtgt aaataacatt ctacactatt gtaattaaat
      301 atgtaaaaag taaaatattt aaatagatat attttaaaat ctaactttta atattgtaaa
      361 aaaattagaa atttaacaaa aagtacaaaa attcacattt ttttcataaa gtgtttgact
      421 aaaaaattca catgttgtag aattaaggta gcaggagttt tgagggaggt gtaaataatg
      481 ataagaaaaa taccacaagc agaactaaaa gtaatgaaat tcatttggaa agtagatgtt
      541 acagtgacat caaaagatgt tattgaagct atggaacaaa aatatggctg gaaacaaacc
      601 accacactga cacttttatc taggctggta aaaagaggat tcttagatgc tcaaaaaata
      661 gacagatata cacattacac aataagagta aaacacaaag attatttaag ttttgagaca
      721 aaggatttcc taagtaatat acatgatgac tctttaagaa gcttaatttc agcattacat
      781 gatgatgaaa atataggtaa agataagcta gattttctag aaagttattt tgataattta
      841 aaggatgaat aattatatgc tatatgtttt tgaaaatctt ataaagacaa ctattgtatg
      901 tagtcttggt atttgtctat tattattttt gaaaagatat ttatttaaga agtttagtaa
      961 gagattcaat tattatattt ggttgataat agtctttaga atgttacttt ttctatttaa
     1021 ttatacaata gtgtatgaag taaaagaacc caaagaaaat gcattaggaa ataatattac
     1081 acaaattgat atttctacag ataataatct tatgctgtat gtagcttatt tatggttatt
     1141 tgtaactata gtcattgcag tttatacatt tataaagtat acaagattta aaaatctagt
     1201 tgttgatgta tcttatgata ttgaagataa tgatataaac tgtatctaca aaaacttgtt
     1261 aaaagaatta aatataaaaa agaaaattga attaagaggt tctgatgaat tgataagtcc
     1321 agcaggcatg ggattattta aatcttacat attcttacca gactattctt atagtaagag
     1381 tgagttaact tggattttga aacatgaatt aatgcatttt aaaaataaag atattttaat
     1441 aaagttttta gttttgtctg ttagaataat atattggttt aatccacttg tttatgtaat
     1501 gagcaataga gtaaatctag attgtgaatt atgttgtgat gaaagtgtta tttataattg
     1561 ttcatttaat gacaaaaaag aatatgcatt ggcacttatt aaatcaatta aactaagtaa
     1621 agaatgcaat agaggtattc tattaacaga ttttaataga accaatttag aaaagagact
     1681 tgaaagtatt attagtaaaa agtttaaaaa tagtgttttt gtagttataa ttttatgttt
     1741 actatcttca ataacatatt ttaaatttga tatatttatt tcaaaaccag tgatttattc
     1801 attaccgaag ccaagtgtgt taaatgatga gtatatttac aacactgata tccgagttga
     1861 aagatgatgt taaattgtat taaaaaatgt aataatttaa ataaaagaaa atattttagg
     1921 aggattttat gttaaaaaaa attaaaaaag tactatcaat agcgtgctgt ttattcatat
     1981 taggagggag tatatctttt gcagatttag aaaataataa tgaaataaat gataaatctg
     2041 ttgagaatcc aatttatgaa tttaatattc aaggttatac atttacatat gaaaatgcac
     2101 cacaaagaat taaagatgaa tataacaaaa catgtagaga attaaatata acaccttctc
     2161 catctactga aatatttatt ccaatagatg aaatctctaa gtatacagat actgttcctc
     2221 tttttgaaat agatgaattc aatgttaaat attttggaac acgttttgaa gttactggtg
     2281 caaaaacata tacagttgga gttagtacat atgtgggata cggacataca actagtggaa
     2341 atccagtaca tttagcacag cttttactaa aaaaatcagg atattctata gcaatagaca
     2401 gtttgtttgg tagttctaca tatagtaaag ttagaagttt tcaaagtaaa tatggactaa
     2461 gtgttgatgg tgtggttgga ttaggaacat ggaataagtt ggcacaagtt actaatgctt
     2521 agataattta ttataaagct aaaaagaaac atgggagtct ggagtctgga aatattcatg
     2581 catattcttt taatgaaatt ttatttttaa aatgacaatt aaatatttat aaataagact
     2641 atgtttataa aaattatagt cttatttata tattttttca aaagttatta ctcctttaag
     2701 gaaaatccat ttagactaga tacataagtt attaatgtat ctttaactcc tattctgcaa
     2761 gtaattttag gtaattcttt gaacttttat tttctctaat taatattact aatgtattct
     2821 ttatatctta taagttgaca tcaatagcta tacactgtgt tatttattat tatactttta
     2881 cttctaaatt tataatatgt ttaattcata aatataattg gataaataat atcaaatgtt
     2941 ctattctatc atccatgaat gaaaggtata cctatatcag taattttagc aattagtgaa
     3001 tgagatatat caattctgca aatactttta aggtaacttg aaatatttaa ggttattttt
     3061 tcaaactctg cttctaacaa attaatgaat tgtacttatg aataaatatt ttaacatatg
     3121 ttgtatattt gtatctattt taatattata ttattaaata aatttagcta atattttgtt
     3181 tttgttaaat ataaaaagtg tctaccataa tttgtatagt aatatttgag attttaatta
     3241 tcactatttt ttgtaagaat acacaattta tatttaaata cctgacagaa tatttaaata
     3301 taaatttgtt ttaaaactaa ttccatataa ctctaccatc tatatcctta acattttgac
     3361 aaccagtcaa aatcatagtc tcacataact cattcctaac cttctctata aaaaactcca
     3421 caccatcaag accaccgcca aaagaagcag taacaaaagg tcttcccatc aaaacagcat
     3481 cagcacccaa tccaagcatc ttaactacat caacaccagt cctaactcca ccatcaacaa
     3541 gtattgttat ctttcccttt acagacttag ctatatctgg aagtacctca caagtaccag
     3601 gagtataatc aagtactctt ccaccatgat ttgaaacaat aatggcacta gcaccagact
     3661 ctacagccat caaagcatca tctacagtca taattccttt tataataaaa ggtaatctag
     3721 tggattcaac caattctcta atctcatcaa ttgttttagg agaaaaagga ttttcttgaa
     3781 attgattatt aataagtcca caagcatcaa ggtctacacc aactgcaaaa gcacctgctt
     3841 cttcagacaa tctaattttt tctattatct ttgaattatt ccaaggcttt ataaatacaa
     3901 ttccatttcc tctattatcc ttcaacacat ctaaattatc cagtaaaaaa gtatctacat
     3961 tagtatcgcc aaccatagca taaattccag aattagaaca acctctaaca acaggctcta
     4021 tgtattcttt ttcggaaact ttaccaccca tgttcaatat agtacctgat acaggtgcag
     4081 cgaatatagg agaactcatt tttctaccaa ataattctat ggatgtatct ggctttgaaa
     4141 cattatgtat gactctcata tttatcttta ttttttctag actttttcta ttttcaatga
     4201 aagacgaacc acttccttta ccacccatac caggaacatc tccagcacaa gctaatccat
     4261 tacaaatttt acatacttta catgttccat taaagttctc tctagctgat tttagtaagt
     4321 cattgtaatt catacaaaat ctctcctttt tataatatta aaattgagta ttactatatt
     4381 gaaaatatct taattgtaaa tatagggatg ttattatgat gagctaaact agataataca
     4441 tattagatat tttttattgt tgtagatagt taaattatat catattagat agtcaaattt
     4501 tgaaaaaaac taacttattt aagtatacat tttgaaaaaa ttaaaaaagc tatatataga
     4561 tttttaaaga aatctatata tagcttttaa gtttatgtca atcttttttg ttaatgaata
     4621 aataatatag aatacaatat acaaaaaagt ttcttgaaat gctaagataa tccccattta
     4681 caaagcaaca tcattatact gtgtaaattt gaatcaacaa tgttttcacc atatttcatc
     4741 ataaaatcct ctaattgtgt gtcaataaat gaataatatc cacttatact atcgatgtat
     4801 gcagtcatta agtgtataca ttcatcactt ttatctttat acatatatat ctccccctat
     4861 atacaatata taaatttaga taccaaatcg tatcatatta aatagttaag tgtgtaacat
     4921 ctatgcagtt atatatttat atcattatat taataactaa agtgagtttt gacaatagat
     4981 tttgggaaac tggatgttca aagtgcaaac ttggatttta aaatcattaa ttaagatagc
     5041 gcataggaat atatgtatac ataacttaat tataaagatt ataaacaatt acttttatta
     5101 actttattta aatggtagaa taacttggag attaataatt ataaggagga tataatatga
     5161 gtttatataa gtttatatat gatgataagg aatatttatt aaaagaggag aactgtagtg
     5221 ctttaataaa tgatgaagaa aatccagttc aaggagtaag tatttcaaaa ataattgata
     5281 tacttaatga agcagaagaa gttgattttg atgtagaata ttatcaagaa gcatgtccat
     5341 tatgtttaga aggagtaaaa gaaaagaaaa aattctttcc ttttttagaa tatcactttt
     5401 atattttcag taaagatgga gaatatgtaa taagcaatat atctgttgat tataaaggac
     5461 tatcttttaa taaactatca aggacaaata aagttgataa tagttatatt gtaagtgtga
     5521 taatttgcga aaattgtcag gattatatag ttcagataga aaattgtata gtttagtgta
     5581 aagtatgtct taatttaaga gaaatttcta aattgagaca tcaacttttt tcaaaaatag
     5641 tatggaaaat tactaaattt atgagataat gtaagtcgga ggtgaattac aatatatagt
     5701 aaatatttgg aagagataga tgatacaatt ttagattcaa tgtttaaact aaccttgaat
     5761 gaaagattat tgactaataa cgattttatt gaaaataaaa ttgatgtagt aaagaattta
     5821 attgattgct ataaaactga acaaaatagt ttaatgttat taaaatcaaa ttttttgcta
     5881 gctttattat gtggcattca agggtttagt gaaaaaatga aagagtatat tttgatttcc
     5941 tgtaaatata ttgataaatg cttacccaaa gattataaat ttttagcacg attttattca
     6001 caaatagcta ttttaagttt aaaaaatgaa gatgtaaata aggcaaattt atatatggat
     6061 aaatttaatc ttatttgtga tgaaaataat tttctaattg aagaaattat ttttaaatca
     6121 caacttattt acattaaggc aagtaagtgt atagaatctt ctgtaataat taaagaaata
     6181 gaaacgttgt atataaagat aaaagaaatt aacgatttta attgtaaaaa aatatatttt
     6241 tttattattg gtaaaatata ttttttgttt ttggatgatg ctctaattgc taaggtaaat
     6301 tttttaaaag caagaaaata tgcagattta ctaaaggata tgcaagtatc ttcactatgt
     6361 aatataaaga taggtgagtg tgaatgttcg tttgaaaatt atgaatgttc catggaatat
     6421 ttcaatgaag taataagcaa taaaaagtat aaaaatgtaa atataattca aaaatataga
     6481 gcatcaaaca acataactaa aatattaata aagactagaa attatcctgt cgcaataaat
     6541 aatttagcaa aatctgaagt atatctagaa aaaattaaaa atcatagctt aaaagaagta
     6601 gagaagtttg atttatttac aagtttagcc atgtattatg caaaaagtaa tgaaaaatct
     6661 tttgaacaat caatatgcta tttagataga gctaaaaaca tattaaatat gataaaaaat
     6721 atagaagaat atataatata tgatttagaa ataatttatc accaggcaaa gatatattat
     6781 atctttggat attatgacaa tgccttaaac acaaataaaa aactccttga aaaatctaaa
     6841 aatgctaaaa attataatta tgtgaagtta gcatacaaga gtatatattt gtgttttgaa
     6901 aagattcaaa attatgaaat gagtatgaaa tactttaaaa tgtattatga gttaaaaatg
     6961 atgtatataa aagctcgaaa tagaaattat atagattcct taaattatag acatgagtat
     7021 atagaaaaag aaataaataa catgagaaaa gtaaaacttg atttagataa aaaaaagtat
     7081 gttgaccatt tgacaagtgc atataataga acttttttga acgatttttt agaaaaacaa
     7141 gaagttagtg aatattgcac agcatttatg atagatgtag attactttaa agaatacaat
     7201 gatacttatg gacactataa tggagatatt gctcttaaaa atataactat aataataaaa
     7261 aaatatctaa aaaaagacat gcttatgata aggtatggtg gagaagaatt tctgatatta
     7321 tctgtatgta aagattataa aaaaagtaaa atatttggaa gaaaattatg taggattgta
     7381 aagaaatttt taaacaataa tttgacagtt agtattggca ttgatacatg taaaaatagt
     7441 agtatagata taaaagaaat aattgaaaat gctgataaag ctttatataa agcaaaggaa
     7501 agtggtagaa atagatgttt gcattatcat gattttaaga atttttaact gttgaaatag
     7561 gattggtgat ttacttgaaa aggagtttaa tagaagattt aaagagaaat ataaacaaga
     7621 tagaatacga tatgttgact aaattctatc taaatttgac agatttaaac tacatcttgg
     7681 attatcacaa agacctttta aatacaaaat tagaagataa tagtagagtc tatactttat
     7741 atttaattag tcttataaac tatataaaaa aagatagaga agcatcatac aactatttga
     7801 tagagtcatt aaagtatatt aaaaaattgt cattaaaaga tgattcagat ataatatcta
     7861 aaatttatat ttatcttact ttatcagcta tttctataag aaaatatgat aaggcaaata
     7921 atttttattt ctcagcaaaa aaaattataa gacaaaatca tttaaatgag ttgttggtat
     7981 tgttaaatat gagtttatgt gtagaaataa gtgctttata tcagaccaaa acggatgtaa
     8041 tagctttagt agaagaagtg tcttttttta aaactttaaa aaataaagct cttgtaagta
     8101 gggcaaatta tatatttggg agagtttatc tatatttatt taataacttt ataaaaagta
     8161 tggaatactt aattgaatgt ctaaaattgg ctcaagaaaa taatttatac tctatagagg
     8221 ctttttgtaa atgtataata agtttgtgtt atttagagag tggaaataat gtagaagcta
     8281 taaaatacat aaataatgta ttagatgttg cacacagtaa taactcaata tcagtaactg
     8341 aaagagtgtc tataaaaata gaccttatat gggcatattt aaaagaagat atgaataaag
     8401 aagcagaagt tattttatta aagactataa aattaatgaa agttgttgaa ggtgtatata
     8461 aggattattt atattcattt atatttttat attcagctga aattgagtta aagaaagata
     8521 attgtagttt tgaaaaggta aacacctatt taattttatc aaaaaatata tataaaagtt
     8581 ttggagatgt ttttatatat aattctaaac tagattacat atataagttg tatggagatt
     8641 tatatataaa atttggtaat atagatgaag gtataagttg ttatataaaa ggtttaaatt
     8701 tagttaaaaa gagttcattg aatcttagaa aaatatctat attttatggg ctaatagcaa
     8761 atggatatga gctaaaaaaa gattttaaac tggcaataga atattataaa agtatggatt
     8821 ggtattctga taggtgggaa aaaaataatt cctataaaac atctcaagca attcacaaac
     8881 aatatgaact cagacaaaaa caagagagtt taagattact agtggatgat aataagaaat
     8941 tagaaaatga tattttaaaa gatggactta ctaaactata taatagaaga tatttggaac
     9001 agcagttgga tttatttaat ggaaccagag acaaaaatat ggtcgcaata ctggtcgata
     9061 tagattgttt taaaagttat aatgataatt atggtcatct agcaggagat aaagttatag
     9121 ttaaggtaac tgatattata agaagtgtat ttatagatat taccgaacac ataatcagat
     9181 atgggggaga agaaatttta atattagtag attctataga atcaatcggt gttgaagaga
     9241 ttgataatat agaattatat attaaaagag ttttcaaact tcttgaattt gagggtattg
     9301 aacatatata ttctaaaata aaaaactata taactataag cgctggagta agtataaaaa
     9361 gatgtcgtag tagaaaagat gttgaaatat taattgaaga tgcagataag aacctatata
     9421 aatcaaaaaa agcaggtaga aatcaataca ttataattta aaattgaggt gattttgttg
     9481 cgaaaaaaaa taggaattat agcctctgat attgaattaa aagaaagaat agaagaatta
     9541 tatagagaag atgtagaaaa tggaactata ataatcgaca tacttaacct agacttaatg
     9601 gaaaatcaag gtcgtatact tgttgaaaaa ggtgcccaag ctataattgg tagaggtgga
     9661 ggttatagtt tagtaataga tacagtaaat gtacctgtta taccaatgaa tatgaagtca
     9721 actgaccttt taagagcaat agaaatagca aagaaatatt ctaaaaaagt agtattgatt
     9781 cttggagaca atgaagtttc ttttgactat gttggctgga gaaatgtcat aagtactgaa
     9841 ataactgaag aatggtttga atctaagtat gagataagaa gtaaagttgt aaaatatata
     9901 gaccaaaaag atgaagttgt aatcgtagga ggaggtttgg catgttcttt tgcaaggcaa
     9961 tatggaattg atagtgtatt tgcaactgct agtgatgaat ctataagaga agctgtagaa
    10021 tattgtaaaa aattattaga tacattaggt gaagaaaagt ttaataatga agtgttacga
    10081 aatatacttg atggtataaa agatggagta attgctatag atagtaatgg cagtataatt
    10141 ttgtacaatg aaagtgcaaa aaacatgctt aaagtagaga gaaaatgtgc acttaataaa
    10201 tacatcttag atgtatttcc taaaatggag tggatgttag attgtttaca tgaaaaagag
    10261 gacgtagaag atagaaagat aagaaatata aataatctaa ttgttaatac taggacaaca
    10321 ttgataaaag ttgataatag tacttatgga gttttaggaa taatacaaga tataaccaag
    10381 ctacaaaact tagaaagaaa aataagattt gacttgaatc aaaaaggtct ctatgctaga
    10441 tatacttttg atgatttttt atttaaagat aaattaacta aagaatttat tgaagaagct
    10501 aaaaaaattg gaaaaagtga ttatacaact ttactttatg gagaaagtgg gtctggtaaa
    10561 gaaattatag ctcatagtat acacaacata agtaaaagaa aagataggcc ttttgttgct
    10621 ataaattgtg ctactatagc agaaaattta cttgaaagtg aactttttgg ctatgaagaa
    10681 ggtgcattta ctggtgctag aaagggtgga aaaagagggc tatttgaact tgctcatgga
    10741 gggactcttt ttttagatga aataaatagt ctatccttta atattcagac aaaactatta
    10801 agagtaatag aggaacgtca aataatgaga attggttctg attatataat acctctggat
    10861 ataaggatta tagcagcaac gaacgaatct ttaaccgaaa aaatagttat gggtacattt
    10921 agagcagact tattttatag attgagtagt ttagagataa atatacctcc tttaagagac
    10981 agaagagaag atataatacc tttgtttaac aattttgtaa atgaagtatt aaaggatgat
    11041 ggtctcaatg gaataaacag tatagatgaa aattttgttt tgactaaaga tgaaatggat
    11101 aagctatata attatagctg gcctggaaat gtaagagagt taaagacaat agctcaaaaa
    11161 tatgttgtga caggtaaaat taaattgaga caagatagag attttaaaac taaaaaatta
    11221 cttcctaact ctgaagtaga caaatttaat tctgaaacga cagcaagtgt agaagtccaa
    11281 gatgaatcta taaatataag taagataaat gatggtaaga taagtataga tataaaagaa
    11341 gtaaataagt atgttgaaga aaaaataata agtatgcttt ttgctcaagg actttctaaa
    11401 aatgaagttg cacaagttct tggaataagc aggacatctt tatggaagaa atacaataaa
    11461 aatatttaaa tataattaca tataaattat tttgaagtaa tgttaatttt ttaaaacaag
    11521 tgttaagaaa aattaacatt acttttttgc aaatttttat ttttttagac aggcatatat
    11581 atttataaga aaaatagtta aaacacttaa tttaataatg aaaaaaataa taattctaaa
    11641 caaaatcaat tttacataaa gtgggattta ttggcatagt aattgcttaa tatataatgt
    11701 aagagtaaat acaattttag gggtgatggt atgagtcaaa gtatggttgg taagcatgca
    11761 atgtggccaa aagaaaatga tgttatattt agcatatctg gcagagctca agcagcagag
    11821 aaggcttttg gtatggataa tgtaataaat gcaacaatag gcgctctaat ggatgattca
    11881 ggaaaattaa taacaatgaa aactgtatat gaagaatata aagcattaga taactgtgag
    11941 ataggtgcat atgcagctct tgaaggtcaa ccagattact tagaagcagt taaaaaagta
    12001 tttttcagag attacttacc agaaggacat ataagagtat tagcttcacc aggaggaagt
    12061 ggtgcaataa aattagctgt atggaactat actaatgaag gagatgaggt attaacatct
    12121 gattggttct ggagtccata tgtaagtata gctgaagagg ctaatagaaa agtagttaat
    12181 taccaacttt ttgatgaaaa tagaaggttt aattttgagt cgtttaaaga aaaatttgta
    12241 aatatagcag aaaaacaagg tagagtcttt acaattataa acacaccagc gcataatcca
    12301 actggatata gtgttgcaga tgatgagtgg gataaaatat tagacttatc aaaagaagtc
    12361 gcaaaagata aagacaaaaa gataatattt ttcgtagatt cagcatatat tgactttgca
    12421 ggtgatgatg atgtatgtag aaagtttttt aagaaatttt ctaatttgcc tgaaaacgtt
    12481 ctcgtattgg ttggattcag tatgtcaaaa ggatttacag cttatggtat gagaatgggg
    12541 gctataattt gtatatcttc aagtgaagat gtagctgaag agttccatta ctcatgtgtt
    12601 cattcatgta gagcaaattg gtcaaattgt aatagaagtg ccatggcagt attatctaac
    12661 attgtaaatg accctaaaaa attcaaagaa tatgaggatg aaaaagaaat atacaaaaac
    12721 atgcttacta gaagagcaga tgtatttgta aaagaagctg aaagagtagg attagaaatt
    12781 cttccatata tagcaggttt ctttgtaagt ataccatgtg ataatccaaa agaagtatgt
    12841 gaagaattga ctaaacataa tttatttgca gttccattaa aaatgggctt aagatttgca
    12901 gtttgtgcag tttctgaaga taaatgtaag aaagcaccaa gtattataaa agaagcactt
    12961 gaaagtttag aagtaaaaat aaataattaa tttcattgaa caaattgggt ggactatgaa
    13021 tttaataatt gtagatggag ggaattttat atgaaacaat tgatgacagg taatgaagca
    13081 attgcacgtg gagcctatga ggctggtgtt aaatatgcat ctgcatatcc agggactcca
    13141 agtacagaaa tattagaaaa tatagcaaca tataaagatg caatagttgc agagtgggct
    13201 ccaaacgaaa aggtagcact tgaggctgct atagggggtt caattgcagg agcaagaaca
    13261 atggcttcta tgaaacatgt tggtttgaat gttgcagctg atccaatatt tacttatgca
    13321 tatacagggg taaatggtgg tatggtactt attacagcag atgagccggg aatgcattca
    13381 tctcaaaatg agcaagacaa taggatgtat gcaaaatttg caaagatacc tttatttgaa
    13441 ccatcaacta gtcaagaagc taaagatatg ataaaagaag cttttgaagt tagtgaaaaa
    13501 tacgatatac cagttttata tagagttaca acaagacttt gtcattcaaa aggattagtg
    13561 gagtgctatg atagagaaga agttgaaatt aaagagtatg ttaaaaatgc taagaaaatg
    13621 gtaactgttc cagcaaatgc tcaaataaga aggggcgttg ttgaagaaag aatggaaatc
    13681 cttaaaaaat tctcaaatga aactgattta aactactatg aaataaatga tacaaaaata
    13741 ggtgttatag cctctggtat gtgttgtaat tttgccaaag aagtttttgg aaaaaatgca
    13801 tcatatatga aactaggatt tacaaatcca ctaccatatg aaaaaataaa agaatttgca
    13861 gaaaaagttg ataaaatata tgttatagaa gaaaatgacc catttataga agaacaaata
    13921 aaagcatatg gaatagattg tattggaaaa gatgtaatac caccatatgg agaaatgaca
    13981 ccagatgttt taagaaaagc tatatttgga aaaacaaatg atactataga atacaaatct
    14041 gaattagtaa caccaagacc accaagtttc tgtgcaggat gtccacatag aggattcttc
    14101 tatgaacttg gaaagagaaa aaatttaata gttggtggag atataggttg ttatactctt
    14161 ggatttgctc ctccatataa tggaatagat tatgttgttt gtatgggttc tgcttttgga
    14221 actgcacatg gagctcaaaa agttcttaat atgaaggatg ataatgaaaa aagattggta
    14281 ggtgtacttg gagactcaac attcttccat acaggaataa atggactttt agatgttgtt
    14341 tacaatagag gtaattctat ttcggttata ttagataata gaataactgg aatgacaggt
    14401 caccaagaaa acccaggttc aggatatact ttacaaggtg ctaaaactaa agaagttgat
    14461 atagaaggat tagttaaagc ttgtggaata gaacatgtaa gagttataaa ccctaacaat
    14521 ttaaaagaag taaatgaagc actagattgg gctttagcta tagaagatga accatcagta
    14581 ataataacaa gatggccatg tgtacttaaa aaattctcta aagaagatat agaagaattt
    14641 aataatccat ttaagactaa gtgtaaagtt gaccatgata aatgtatagg atgtaaatta
    14701 tgtttgaaaa ctggatgtcc agctctatca tttgataaag agaataagtt atcaaatata
    14761 gatagaaatc agtgtgtagg atgtggagta tgtgcacaag tttgtccaaa acaagcaata
    14821 gttaaggagg aaaaataata tgactaagag tatacttttg gtaggtgtag gaggtcaagg
    14881 aactatactt gctagtaaat tattaactat gggattgatg gaagctggat atgatgtaaa
    14941 gatgagtgaa atacatggaa tgagtcaaag aggaggttca gtttcctcac aagttagata
    15001 tggagattgt gttcattctc cagttataga aattggtgga gcagatatat tagtatcttt
    15061 tgaaaaaatg gaagctttaa gatggttcaa ttatttaaaa ccagaaggaa aagctttagt
    15121 aaataatcat aggatagatt ctatgactgt tttaatcgga ggagctgaat atcaagaaag
    15181 tgatatagat gcagagttaa gtagattaaa tgcaaaagtt ataaatgcag ctgataaagc
    15241 tgaagaacta ggaaattcaa aaataatgaa tgttatttta ttaggatgtc tagttaaatc
    15301 tatgcaatta gagtctattg attgggaaaa aattataagt gaaaatgtta aaccaaaatt
    15361 tgttgaatta aacataaaag ctttccatga aggtatggaa atggttagca aatagtttat
    15421 aatagatata aatagactat agaaattaag gtgaaaggaa gaaaattatg acttacagaa
    15481 tattagccat aaatccaggt tctacttcta caaaaatagc agtatatgat ggagaagaac
    15541 aaattcttgt gaagacgata gaccatccag ctgaagagat tgcaaaatat aatactatac
    15601 aagaccagtt tgaaatgcgt aaggaagcag ttttgaatat tcttaaagaa aatagtatag
    15661 acttaaaatc tcttagtgca atagtaggaa gaggtggagt tttaccacca gtaaaatcag
    15721 gagcatattt agtaaatgaa gaaatgattg atgtactaag acatagacca gtacttgaac
    15781 acgcttccaa tttaggtgct gttgtagcac atgcaatatc agaacctctt ggaatcaact
    15841 catatattta tgattctgtt gcagtagatg agcttataga tgtagcgaga atatctggac
    15901 tttgtggaat ggatagatca agtgcagggc atgcattaaa tactagagca atggctttaa
    15961 aatatgctaa ggataaagga aaagattata agagcttaaa cttaatagta gctcacattg
    16021 gtggaggagt aagtatttat cttcatgaaa aaggaagaat ggttgatatg ctatctgatg
    16081 atgaaggacc attttctcca gaaaggtcag gaagagtacc tgctacaaaa ttagtggctg
    16141 cctgttattc aggtcaatat tcagaaagag aaatgactaa aaagataaga ggtaaaggtg
    16201 gtatagtttc atacctaaat actgtagatg ctagagaagt tgaaaaaatg atagcagaag
    16261 gaaatgaaga agcaaaaatt atttatgaag caatggctta tcagttagca aaaggtattg
    16321 gagagttagc aactgtagta gatggaaagg tagatgctat aattataaca ggtggaattg
    16381 catattctga aatgtttact tcaatggtta aaaagaaagt tgagtttata gcaccagtag
    16441 aaattatggc aggagaaaat gagttggaat cacttgcttt tggaacttta agagtactaa
    16501 atggagaaga agaagctaga atttatagtg aaaattaata agaaaaacta aaaatgttta
    16561 tatactaaaa aagtggtata tccgtttttt atacacgaaa gcttagagta taggtgaaaa
    16621 tgtataaaat ttggattgcc actttttata attaaaattt tatcttaacc taataaataa
    16681 ttctgtaaaa tttcgtattt atatatatat tttattggta tttattgtta ttattattat
    16741 gaggagtgat ttttgatggg aaaaataagt ttaaaaagta tgataagatt attttcaggt
    16801 ttttttatat ttgcaatttc ttctgtgctt atgattaatg cacatgtagg tcttatgcct
    16861 tgggatgtac ttcaccaagg tttatctata aagctaggta taactatagg tcaggcttct
    16921 ataatggtag gagttgtaat tgttatattg gatgcagttt ttggtgaaaa tataggatgg
    16981 ggtactttac tcaacatgac atttatagga atttttattg atttagtaat attttctgga
    17041 gtgatacctc atgcaaacaa cacttatata ggtgtgttta tggttgttat aggaattatt
    17101 ttggcagcta tagcaagttt tctatactta ggtgtttgtt taggaagtgg accgagagat
    17161 gggcttatga ttgctcttca gaaaaaaact aataaatctg tacgtcttgt tagaacaatt
    17221 cttgaaatac tagcattagt tgttgggtgg ttattaggag gttctgtagg tattggtacg
    17281 cttgtaagtg ctttaggttt aggatatgta ttacaaatag tgtttagaat atttaaattt
    17341 gacacaaaat tgctgaaaca tagatttatt atagatgata taagagaatg gaaggaaaag
    17401 aaaagcaatg aacataaatg taaatcaagt attgtaataa aaaatgagca gaattaatta
    17461 tgctaaaata tatttgtagt ttatctaatt tatagtaaag tcaaggagat tttgaaatgg
    17521 aattatggga tttatataat gctgatggaa ttaaaacagg aaatgttata aaaagaggta
    17581 attctattga agaaggttat taccatttgg cagttgaagt gtggatttta aatagtaact
    17641 ctcaaatttt aattcagaaa aggtctaaaa gtaaaaagac attgccaaat atgtggggaa
    17701 tgacaactgg atgtattgtt tctggggaag acagtttaga aggagcaatt cgcgaagcaa
    17761 aggaagaaat agggatagat ataactaaag atgagatgaa agtttttaga agtatgattc
    17821 atgaagatac tctttgggat gtatacttag ttaaaaaaga atatgatata tctaaagcta
    17881 ttttacagga ggaagaagta agtgatataa agtgggtaag cacagatgaa ataagacaat
    17941 tactaaaaga gggtttgttt tttgaatatc cagagatata cgaattgcta tatgagatag
    18001 ataataataa attaaatata tgatgtagag cagatttcag tataagtaag attatttaaa
    18061 atgtatcttc tctaaatttt aatattgttt tatgaaaatt aataagttat ttttagtatt
    18121 tttctacttg ttttttgaga ttaaaataaa aagggcagtc attataaagt cataaaaatt
    18181 tggggaggat ttaaactatg gaagataaca aaggattggc acgtgacagt tcaaaatata
    18241 aaattgtttc tatatctgtc gtagtaatta ttctaattac attttggtat atgttaaata
    18301 tggtgctttt aacatttatt atgactttta ttttctataa cttgcttgta gccacaagaa
    18361 aacgaattaa gaagttttct tctttaaata ttccagattc attgataatt atagtgttat
    18421 atgcactatt tgctatattg ttagtattga ttagctatgc agttgtacca ataattatag
    18481 ttcaacttac agagttgagt agggttttta gtgattttga tgtaaatcaa tttgctcaat
    18541 ctttaggacc aaaattgtat cctattgtct caaagctgga ttttaataaa tatatttcac
    18601 aagcagggct acttatagca tcaacagcaa caaaagttgg aagttttgga gtaaatatat
    18661 tattggcatt tttactgagt ttactccttt tacttgaaaa aaatgaaatt aaaaattttg
    18721 gagataaact ttctgatagt aagatttcct ttatttataa ttcgcttgta ttttttggaa
    18781 agagctttgt taaaaatttt ggtgaagtaa tgaaagtaca agttatgatt gcctttataa
    18841 attctgttgt ttctatgatt tttttaggtt tcatgggatt ccctcaaata tgggctttag
    18901 gatttatgat ttttgtgttg ggtcttattc cagtagcagg agttatagtg tctttgattc
    18961 cattaacggt aatagcattt aatacaggag gaataacaaa ggtttttgga gttttactta
    19021 tgatttgtat agtgcatgca gtagaaacat acatattaaa ccctaagttg atgtcgaata
    19081 gaactaaact tccagtttgt tttgttttta ttattttact tgtaggagaa cattatttag
    19141 gtgtatgggg attactaatt ggagttccaa tatttatgtt ccttatggat attctgggtg
    19201 ttaaatttac ttctaggtaa tatattaaaa taataattta tttttatata taaattatga
    19261 attgttttaa aaaagaacct ataaatagac atcattaata aagacatcta tttgataggt
    19321 tcaattttgc tttgtataaa tactataaaa ttaaataaaa agaaatttat ttcatttcaa
    19381 aagtttcaca acttgcattt ctttcattta tatgaacttt ttcagctttg caaagttcat
    19441 ttttattgta tttacatttt acagcttcac aatgaatgtt atctgtatca acttggttag
    19501 tttcattaga acgactagtt ataccagatg tatatttgtc ttcaaaagaa gcacaataag
    19561 tattttctgt agtagttgct tgcattccat ctacttttat actaccagca taacaatatc
    19621 cactagtatt gtaagcacaa ttagtagcag cacaatttaa attattattt ttcataaata
    19681 acctcctaag ttttatttaa acttattatg tgaagttatc taaattttat acacttattt
    19741 acaattacat ttatcacatt taacaactac atctttggaa gtttcccttc ctaaattttt
    19801 attaacatga tacacatcaa cattttcagt tgatccacat atagaacatt taaaattgtt
    19861 ttctagtagg gttctttctt taaataactt ccaatacata gaattgatgt aatcattata
    19921 atttaccttg aggcatgtct caagaaagtt tatgtataga ttgtctaaat ctcctgtatt
    19981 taattctgta tattctggtt caaaatattc acaacatcct ctaaaatcag gctttgtatc
    20041 tgaaataaca catctaatat catatctata gtctaggcta ggccccttaa atgaaagatg
    20101 tgtgcaaaaa gaacaagatg tttctggtag atttttttca agttcatgta atttattatt
    20161 cataagtatt cctccaaaaa atttatttta agtatataaa tgaatagatt attttaaaac
    20221 ttttatttat ttatattaga gatttaggat aaatattcca ataaggagta aatacttaag
    20281 tatttattac tgtattaaat aaaaaagagg caaatctaac ttgagattgc ctactttttt
    20341 atcatttttt aataaaattt attctaagta ttattttatt tagatttagt cagccaactt
    20401 agtatctgct ttgcttttta actttctacc agagcgtata gttaaaatag catataatac
    20461 agtaaatatg gcagctaaaa catatgatat atttatgttt aagttaaatc caatttgagc
    20521 attaaatata taactgaata taacaaagga ataaaacata ccaggaatta agcttataat
    20581 ataattcttt tcttttatta taaggtaaac tgtaatcatt gcaaatgcaa atatggcaat
    20641 agtttgatta ctccaagaga agtatctcca caatatatta aatccagaag cactaagctt
    20701 tgcaaatata aggatggcta tgacaggaat aaatatacag attgacaata taacacgatg
    20761 tttcttttcc ttttggtcat aatgaaagta atctgcaagc attaatctta aagatcttaa
    20821 agctgtatct ccagaagtta ttggtaaaac aattacacct attatagcta tgataccacc
    20881 aatagaacca agtaaatctc ttgcaaccaa acctattaca tcaggagaac ctactaattc
    20941 tttaggtatt cctttattgt aaatacccat agcagctgca gcccaaatca ttgcaattaa
    21001 accttctaaa atcatcatat tataaaaagt agtttttcct tctttttcat ttgaaactga
    21061 tcttgctata agagtagctt gagttgagtg gaatccagaa acaataccac aagcaactgt
    21121 aacaaagaat gtaggaataa gaggaatacc atcaggatga atacctttcc aatttgcaag
    21181 acttaaatta gctaaatcat aaccttgtgt aaagattcca attccaacac cagcagcaga
    21241 taaaagtaga atagctccaa atattggata tacctttcct atgattttat caattggaaa
    21301 tagtgttgca cacaagtaat atagaaagat aagtccataa actatccaga caactggatt
    21361 atttatagtc gatttcatat ttaaaatttg agtaacaact aaatccccag gtgtataaat
    21421 aaaaactgca ccaactaaaa gcattaaaag acaaaggaat atattataga cttgaaatac
    21481 tttattacct aaaaatctac tgataagaga aggcatttgt gctccttttt ctcttatact
    21541 aatcattcca ctcatatagt catgcaaagc tcctccaaat acacagccaa taggtattag
    21601 tatgaatgct attggaccaa acaatatacc ttggataggt cctagaattg gtcctgtacc
    21661 agctatatta agcaactcaa ttagactatt tttccatttt ttcattggga catagtctac
    21721 tccatcactt tgagcaagtg caggtgtttt tctgtcatct gggccaaata ctttttcaca
    21781 ataagcaccg tataaagcac ctcctattag taatatagcc aagccgatta agaatgttgt
    21841 cataatatca ccccatttat aaaatttttt ggtctttgta tacatattat ctaaatgtgt
    21901 agatttatat tgaatttaat tctggacaac ctacaaattc tataaagtat tacaactaat
    21961 ataatttcag aaataaatac atatacataa ccatgtactt agtatacagg tatttggtaa
    22021 tattttcaat gttatagcaa taaagaatat aaacaaaatt ctgataatat tttacaaaaa
    22081 aattaaaaaa tataaaaatt agtttaaata gatggagaaa aaaggaaaat caattgagaa
    22141 tataaatgtc tatatcttaa caatattaag aggtttaaaa ttcttgaaga aagaatagtc
    22201 aagtgatata atgagtttac atgtgtatat acactagtac atatagaaag cggtgcaatt
    22261 atggataaag atttaacata tcaaatcaaa gagctaaaga aagaaaaaaa tgcaataata
    22321 cttgcccatt tttatcaacc accagaaata caagaattag cagatgcagt tggtgactca
    22381 tattatttaa gtgagattgc tagagattgt aaagaagaag tggttgtatt ttgtggagta
    22441 aggtttatgg gagaaagtgc taagatatta tcacctgaaa aaactgtatt gatgccagta
    22501 agtaacgctg ggtgtgctat ggcagatatg gtagatgaag aaggcgtgat aaaattaaaa
    22561 caacagtatc caaatgcttt ggttgtatgt tatataaact ctacagctaa agttaaggca
    22621 cattgtgatg tttctgttac atcttctagt gcaattaaaa tactagaaaa catagataat
    22681 aaagaaatta tattccttcc agataaaaat ttaggaggat atattgcaga acaatttcca
    22741 gataaaaact ttatattttg ggacggatat tgtaaatacc ataacaatat aagagctgaa
    22801 gaaataattg agttaaaaga taagtataaa aatgcagaag tattagtaca tccagaatgt
    22861 aaaaaagaaa taagagattt aggagattat gtaggtagta catcaggaat aataaaatat
    22921 gctactaata gtaaaaacaa agactttata atagcaactg aagaaggtat attacatgaa
    22981 ttaaagaaaa ataatcctaa taaaaacttt tatattcctg gtggaaaaat actatgtaca
    23041 gatatgaaaa agacaacatt agaaaattta tatagtacac ttaaaaatat ggaaaatgaa
    23101 gttattgtag aagatgaaat catggaaaag gctttaaatt cactgcttaa catgcacaaa
    23161 ttagcagaag gatagaggga tagacttatg aatttagaac aagatgtttt aatagttgga
    23221 agtggagttt caggattata ctgtgcatta aatttagata aaagtttaaa tgtgctagta
    23281 gtatcgaaat caacaattga aaacaacaat acatatcttg cacaaggtgg aatttcaact
    23341 gctagaaata ttgatgatat agaatccttt gtagaagata caatgaaggc tgggcagtat
    23401 aaaaatagag ttgaagcagt tcaagtactt gcagatgaat ctatagaaaa tgtagggcag
    23461 atagtagaat atggtatgcc tttagacaaa gaaaatggag agatagatta cacaagagaa
    23521 ggagcacata gtgtaaatcg aatagttcat agtaaggata atactggaga agttgttttt
    23581 aaaactttat taaaagaagc taaaactaga gaaaatataa ctcttataga agatgcctat
    23641 cttttagata tattaaaaga tggcaataaa tgtataggag ctagaatttt taaatctaaa
    23701 aaagagattc atgttttttc aaaaatagta gttttagcta caggtggaat tggtgggctt
    23761 tttaaaaatt ctactaatca aagacatctt acaggtgatg gaattgcaat agctttaaga
    23821 aataatataa aaatagaaaa tttagaatat attcaaatac atcctacagc cttttatgaa
    23881 gaaaataatg aaggtaggag aatgcttata tctgaatcat taagaggaga gggagctaaa
    23941 cttttaaata aaaataaaga aagatttgtg gatgaacttc ttcctagaga tgtagtttca
    24001 aaagctattt ttgagcaaat ggaaaaagat aaattacctt atgtgtattt agatgcaaca
    24061 catttagata gtgaatattt aattaataga ttttctttta tttataatga atgtttagct
    24121 agaggaacag acataacgaa agaatgtata aaggtatctc ctgcacaaca ttattttatg
    24181 ggcgggatac atgtagattt ggattcaaaa acatctatgg aaaatttata tgcagttggt
    24241 gaaattagtt gtacaggtgt tcatggagcc aatagattgg caagtaattc attacttgaa
    24301 gggcttgtat tttcaaaaag agcagctaaa aatataaact cagttataga taatgtaaaa
    24361 gttaagttta tagatgtacc agatatggat atagatatag aacaggtaaa aaaagaaaat
    24421 aaaatattag tcataaaaga aattgaaaga acaagtgagg attttggcga tgaattattt
    24481 gattattgat agaatgatac aggatgcttt gatagaagat gttccaagtg aagatataac
    24541 aactaattct attgtggatg aaaattctaa atctactgta gatttaattt gcaaacaaga
    24601 tggtataata gctggcttag gtgtatttaa gagagttttt gagatacttg gtgatgtaga
    24661 tgtaaaatta tataaaaatg atggtgatag agtaaagaat cgtgaaaaga tagctgtatt
    24721 aacaggtagt actagaaact tacttgtagg agagcgtgta gcattgaatt atttacagag
    24781 aatgagtggt atagctacta ttacaagtag gtatgttgaa aagcttgaag gtacaaatac
    24841 aaaattatta gatagtagaa aaacgatacc taatcttaga atattagata agtattctgt
    24901 caaagtaggt ggaggatgca atcatagatt taatttatct gatggaatat tgcttaaaga
    24961 caaccatatt ggggctgctg gtagtgttaa gaaggcagtt gaattagcta gaaaaaatac
    25021 ttcttttgtt agaaagatag aagtggaagt ggaaacttta gacatggtaa aagaagcaat
    25081 agaagctaat gcagatataa taatgttgga taatatgagt ttggaaatgg caaaacaagc
    25141 agtagatatt ataaatggaa gagccattgt agaattttcc ggaaatgtaa atttagatac
    25201 tatagaagat ataggaaaaa ttggtgtgga tgttgtttct gtaggagcgc ttacacattc
    25261 tgttaaagct ctcgatataa gtatgaagaa tttgagaaat atataaattt tatattgaaa
    25321 taaacttaat taaaatagac gccttaaatt tgttttataa atttaaggag tctatttttt
    25381 attcattaaa attaataaaa ata
//
LOCUS       DAEMHW010000042        25389 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEMHW010000042 DAEMHW010000000
VERSION     DAEMHW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.042"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            341..2197
                     /locus_tag="KPK67_001462"
     CDS             341..2197
                     /locus_tag="KPK67_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG2458454.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGIGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    2280..2520
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2586..2821
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2906..4123
                     /locus_tag="KPK67_001463"
     CDS             2906..4123
                     /locus_tag="KPK67_001463"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG2458455.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            4240..4722
                     /gene="cdeM"
                     /locus_tag="KPK67_001464"
     CDS             4240..4722
                     /gene="cdeM"
                     /locus_tag="KPK67_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBG2458456.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            4987..6252
                     /gene="hisD"
                     /locus_tag="KPK67_001465"
     CDS             4987..6252
                     /gene="hisD"
                     /locus_tag="KPK67_001465"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBG2458457.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6455..7207)
                     /locus_tag="KPK67_001466"
     CDS             complement(6455..7207)
                     /locus_tag="KPK67_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2458458.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCQGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFNY"
     gene            complement(7325..7594)
                     /locus_tag="KPK67_001467"
     CDS             complement(7325..7594)
                     /locus_tag="KPK67_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458459.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            8069..9163
                     /locus_tag="KPK67_001468"
     CDS             8069..9163
                     /locus_tag="KPK67_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG2458460.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVIKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            9165..10115
                     /locus_tag="KPK67_001469"
     CDS             9165..10115
                     /locus_tag="KPK67_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBG2458461.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIIDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            10108..11282
                     /locus_tag="KPK67_001470"
                     /pseudo
     CDS             10108..11282
                     /locus_tag="KPK67_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11295..12791
                     /locus_tag="KPK67_001471"
     CDS             11295..12791
                     /locus_tag="KPK67_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG2458462.1"
                     /translation="MGKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEETPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12814..13764
                     /locus_tag="KPK67_001472"
     CDS             12814..13764
                     /locus_tag="KPK67_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2458463.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13785..14747
                     /locus_tag="KPK67_001473"
     CDS             13785..14747
                     /locus_tag="KPK67_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG2458464.1"
                     /translation="MKKLMKHLALLLSLVLIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGFKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            15119..15751
                     /locus_tag="KPK67_001474"
     CDS             15119..15751
                     /locus_tag="KPK67_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2458465.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            16162..16254
                     /gene="kdpF"
                     /locus_tag="KPK67_001475"
     CDS             16162..16254
                     /gene="kdpF"
                     /locus_tag="KPK67_001475"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBG2458466.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            16295..17977
                     /gene="kdpA"
                     /locus_tag="KPK67_001476"
     CDS             16295..17977
                     /gene="kdpA"
                     /locus_tag="KPK67_001476"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBG2458467.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            18002..20059
                     /gene="kdpB"
                     /locus_tag="KPK67_001477"
     CDS             18002..20059
                     /gene="kdpB"
                     /locus_tag="KPK67_001477"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBG2458468.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            20078..20704
                     /locus_tag="KPK67_001478"
     CDS             20078..20704
                     /locus_tag="KPK67_001478"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBG2458469.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEQFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            20941..21849
                     /gene="cysK"
                     /locus_tag="KPK67_001479"
     CDS             20941..21849
                     /gene="cysK"
                     /locus_tag="KPK67_001479"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBG2458470.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21876..22466
                     /gene="cysE"
                     /locus_tag="KPK67_001480"
     CDS             21876..22466
                     /gene="cysE"
                     /locus_tag="KPK67_001480"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBG2458471.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22626..22814
                     /locus_tag="KPK67_001481"
     CDS             22626..22814
                     /locus_tag="KPK67_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG2458472.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22868..23737)
                     /locus_tag="KPK67_001482"
     CDS             complement(22868..23737)
                     /locus_tag="KPK67_001482"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBG2458473.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23934..25178)
                     /locus_tag="KPK67_001483"
     CDS             complement(23934..25178)
                     /locus_tag="KPK67_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458474.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNVLDLEDIHSYSIENNTNITIYENNTLQYKFNEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT         9793 a         2700 c         4545 g         8351 t
ORIGIN      
        1 actattgata agtaagagat aaaattggta agtatcaaaa aatatactaa ataaaaatta
       61 tagtcctaat gagttaattt ctgtattttc aaataatgca gtatttagtc atgtgttttt
      121 atatgcgcct gtatttctaa ttaccaatat attagaataa gctactgtaa ttagatttag
      181 aaaaaagcga cataaataat tataaacaaa taataaatta cataaaatta aatttattca
      241 caagtatatc ttataaaata tgttataatt aatagaaaat tgtcatataa ttaattaagt
      301 agtattatta taaataacat tgaaaggtag tggttagaat atggataccc ataataaata
      361 tgtaaatttt ataaaaaata tacctgtacc ttttttatat tgtagaattg taaagagaca
      421 agaagatata gaatatcgag tagaatatat aagtaagggt ataggcaaag tattacaatt
      481 agaagaaggt atttgtgata aaaatatact agatgtattg cctgtattta agtctaaaaa
      541 atatttcaaa gagttgttct caaatgaagt ggattgtata aaaaggtaca ttccgacact
      601 taaaaactgg ataaatataa aaaaacagat aataggggat tcatacataa ttttgtattt
      661 tggtaaaatt gtatttgatt atagacagat aatcgattcc tttgataaaa aggaaaaagt
      721 agcttatatt aaagacgaag aaggcatata tatagattgc agtgaaaatt taataccaat
      781 attaaataac aacattaaaa caacgaaaga tatctttggt aaaaatgata tagaagtatg
      841 gggagagaat acaggaaaat tatttagaga tgattataga gaaggagtat ctagtaaaaa
      901 aagattttta caaaatcttt ttgaatatga agaaacattt tttatggttg aaaaatattt
      961 tttgtatgat gaagacgaac ttttaggtac tatagggata gtagacaata taatatatag
     1021 tggttacagt aatagaaatt ataactcaaa agatttaatg aagatgatag aacactcaat
     1081 tccagagaat atgttttata aagatgtgta tggaaattat attggattta attcaggttt
     1141 tttgaattta gcttgtatga ataaagaaga attattaggg aagaatagtt ataaaatatc
     1201 agaagaagaa gctttaatag ataagatatt tgaaagtgat aagggtgtag ttgaaaataa
     1261 aaaagtagtt acatttgagt taaatataag tatgaatgat gaaaacaagt gtattgagat
     1321 tacaaaaagg ccgttttttg atagctatgg aagtgttatt ggaataattg gaacagcaag
     1381 ggatataagt agaagaaaaa gattagaaga ggaaatggat aaaactagga tggaattttt
     1441 tgcaaattta tcccatgaac ttagaacgcc tataaattta atatcatctt cattacaagt
     1501 gatagaaaaa aaagaagcag atttaataga atcaaatgat actttaaaaa gaaatttagg
     1561 tataataaaa caaaatggaa atagaatact gaggttagta aataatgtta tagattttac
     1621 aaaaatgcaa tcaggatatt tagattttaa accagaagaa tctgatataa ttgcatttat
     1681 agaggagata tgtatgtctg tagctgattt tgcaagccaa aataatatac aacttacatt
     1741 tgatacagaa atagaagaat tttcaatgtt atttgattca gaaaaattgg aaagaataat
     1801 attaaattta ctttctaatg gaattaagta caataagaaa gatggaaaaa taaatatatt
     1861 tttatatgtg aaagataatg tatttaatat gaaaatatct gatagtggaa taggaatacc
     1921 aaaagaaaaa atagataaaa tatttaatag gtttgaacag attgacaatg aactttctta
     1981 cagagttaaa ggaagtggta ttggactttc attagtgaaa tctttggttg agttgcatga
     2041 gggaagtata tctttaaaaa gtcaacttgg tattggaagt gaatttatag tttcattgcc
     2101 tgttagaagt aaaaataata ttgaaaaata taatcataaa agagaaatca gcaatgaact
     2161 aagtaaaaaa ttagaaatag agttttcgga tttataagtt taaatataaa aaataaaatt
     2221 ttaaaaaagt attgacaatt gatttagaag ggtttaaaat aaattacaac ataataaata
     2281 aaaaggcgat gaataggaag agtaaatcta attcgattac cagagagaag agtccattgg
     2341 ctggaagact ttttaaatta gagggattga aaactaccta ggagctactt tctgaagtca
     2401 tctgcattag tagattttag taagaatagt cgttagctgc gttaaagtgt aaagtgagtt
     2461 ttcatgacat atgaaaacta aattaggtgg aaccgcggga ataatactct cgtccttatg
     2521 ttataaggat gagagttttt tgttatttaa aaatataaac agtaatcata ataattatag
     2581 aaaatataaa agcaatgaat aggaagagta aatttaattc gactatcaga gaggaaagtt
     2641 cacaggctga aagactttct aagtttgaga aattgaaaac tacctaggag ctgtggtctg
     2701 aagttacttt aaaaagtttt agtaagacta accgttaact gcgttaaagt gtaaagtgga
     2761 ttttatcaat aaatgataaa atcaaattgg gtggaaccgc gagtatagac tcgtcccttt
     2821 atatagggat gagtctattt ttattgcaat aaaagattga aaataaaaaa aaatcggaaa
     2881 attataaatt taggaggaaa ataaaatgga gaataaagtg aaaattggag tactaggata
     2941 tggagttgta ggaagtggac ttatagatat aatagataat aataaagaaa aaagaaatat
     3001 agaaatagtt ggaattttag ttaataattt ggaaaaacat aaggacaaaa aatattctaa
     3061 tataataact aataatatag atgatatatt taataaagat atagatatac ttgttgaagt
     3121 catgggtggt ttagaaccat cattatctta tataaaaaaa gcattaaaca ataaaattca
     3181 tgttgttaca gcaaataaag atttgttagc tgaatgtgga gatgagcttg caaaattagc
     3241 aagtgaaaat aaagtaagta ttaaatttga agcttcagtt gcaggaggta taccagtatt
     3301 aaaaccaata atagaatcac tagaaggaaa taatatagat agtataaatg caattttaaa
     3361 tggaactact aattttatat tatctaaaat gtatgatgag aatttatctt atgatatggc
     3421 attaagacaa gcacaagaat taggttttgc tgaagcaaat ccagagtcag atgtattagg
     3481 gtatgatgct gctagaaaac tatcaatatt atcaactcta gcttatgaca atagagtata
     3541 ttggaaagat gtgtatttag aaggaattac agatatagat gaaaaagata ttgaatatgc
     3601 aaaaaaatta aattgtaaaa taaaattgat tggtcaaagt aagtatgaaa atgataaagt
     3661 aagtgctttt gttagacctg ttttagtaga aaaagataat atccttgcta gaatagacaa
     3721 tgagttcaat gctgtcatag tgaatggaga ttcagtagga gaagtttctt ttgtaggtaa
     3781 aggagctgga agtttagcaa caggaagtgc ggtttattca gatgttatag atataataga
     3841 taatagagtt tcaagtatag attcttttac taaagataaa atacaagtta ataaaatagt
     3901 tagagaaaag tgtggagcac ttcttagatt taaaaaatgt aataaagatg aaattctaaa
     3961 tatagttgag aattgtttag taaaattcga tattttaaat gatgatgatg aacttgctat
     4021 tatggtttat gctgattctg aatatgaaat aaacaacagt ttatgtctaa taaaagataa
     4081 aggatactgc gaaaaaatga ataaaatgtt gaaaataagt taaaaaagaa aaaatataag
     4141 tttatatgta acatattttg acgattttaa tactatatag tatgagatga aacaaaacat
     4201 ctcataaaaa aattaaaatt aaagggagag atatgttata tggaaaataa aaaatgttat
     4261 tcagaagatt ggtatgaaag aggagaatct acagctaaat ggttccaaaa tgatagagaa
     4321 gaatatgaaa gagaagcata tgatgaagat agagaaagaa gaggttcaaa ctgtggatgt
     4381 tcagattcag gagaaaatag acctagaaac tgtgaaagat ttagaagaga agctgagata
     4441 agagaaagag aagcaagaga agcattctgt gaatcttcag agaaaaagaa agaggcatta
     4501 gcatatgaat gtgaagctag aaaattatgg gaagaagcag aaaaatactg ggatgaatat
     4561 tcaaaataca actataaagg aatcgaatat ttagcagaag ctgctagatt atttgatgaa
     4621 ggtatggaat gtgaagctag aagaaatgga aataatggag gaaacaataa taattgttgc
     4681 cataaatgcc ataaatgtaa ttgtaactgc tgtagaaaat aaaaaaatta attgacaaat
     4741 caatagttaa aatatatcat attatgtata ataaagagaa aattcagaaa aaaataatga
     4801 ttagaagaaa tataagtata tttattagac aaatagtgct cagttagtga ggtgagtaca
     4861 atatgattaa actcccatcc tctagctttt gatagagggt gggagttttt atttatttat
     4921 atatcttaaa tgaggataat attgtaaaat aaatgagtat gaaattgtaa aattaggagg
     4981 taaaaaatga aaagtattaa aacatcaagt atgaaagaaa aggtagatga aaagatatta
     5041 agagagcaag taagttctat aatagaagat ataagaaata ataaggatgt tgcacttaaa
     5101 aaatataatg agaagtttga tagaaataca agagatgaat ttagaattac taaggaagaa
     5161 ataaaggaag cttataagca tgtagatgac gaatttataa ataacttaaa gattgctgcc
     5221 aaaaatataa gagaatttgc taaagctcaa aaactaagtt ttgaaaaccc ttttgaaaaa
     5281 gaaatttatc caggtgtaat cttaggtcaa actaatattc caatagagtc atgtcttgca
     5341 tatgttccag gaggtcagta tccattgttt tcaacagctc ttatgcttat aatacctgca
     5401 aaagtagcag gagtaaaaag aatagtggca tgttcaccaa ctatgaaaaa tactgaaaag
     5461 ataaatccta agacattggt agctatggat atagcaggtg ctgatgaaat atatgcaact
     5521 ggaggagttc aagctatagc agcatttaca tatggaacag aaaagattaa tccagttgac
     5581 ataattgtag gaccaggaaa taagtttgta actgaagcta aaagacaatg ttatggacaa
     5641 gtagggattg attttgtcgc tgggccaagt gaagttctta taatagcgga tgaaacttca
     5701 aatccagttt atattgcagc tgatttatta gcacaatgtg agcatgattt aaatgcaaga
     5761 ggaatattat tgacaaatag cttggaagta gcacaaaaag ttgaaaaaaa tatagaaagt
     5821 atgctaaaag atttgccaac aaaggacata gcatatagtt catgggaaaa taatggagag
     5881 ataatattgg ttgatgatat ggaagaagcc attaaaataa gtaattttta tgcaccagaa
     5941 catttagaag ttgcagtaaa tgagtataat gatatttgtg atagattaac taactatggt
     6001 tctttattta ttggaaatct ttctgctgag gtgtttggtg actatgtttc aggtaccaat
     6061 catacattac caacacttaa agcatcaaga tatactggtg gtgtttgggt tggaactttt
     6121 atcaaaactt gtacaaagca gatatttaat gaaaaagcta tacaatcatt agcacctgta
     6181 gctgaaaagc tggcaaaaga agagggatta tatgcacatg caaaggctgc tgaggttagg
     6241 tttaaaaagt agtaaaaaat tttattttgg cagtcgagat ttagtattag actgagataa
     6301 ggaaaacgct gagaacccga ataaatacta tattttgtgg gcctcggcgt ttcttttata
     6361 tctgtatata ataggataat actacctgtg tgtttaaata ctaacttaat atttcttttc
     6421 taaataatgt ttatgaaata gttaatattt ttagttaata gttgaaaaaa acttttgcta
     6481 actgtgcaaa ttcatctgca tttgtcaata tagcagggtg tcctccctga ctaaaaatgt
     6541 gaatacttgc ttttgaaata aaagttgcca tttgtttgta ctcttcttca agattatgac
     6601 gacacatttc gtcttcctta cttcctgtaa ataaaattgg catttctaat ttacacaatt
     6661 ctttatgaaa caaagggcgt ttctcgtttg cacattgcaa tagagctttt gtatcaagat
     6721 taacaacatt ctcccaatct tttccctgac accattcata gaattttcgt gcttgaatat
     6781 cttgctttga tttttccctt ccagtgatta gattatctat gaagttatca ttaagagtac
     6841 gtccatcaaa gctatctgct ataattgctt caactaaatc gggacgttct aatccagtat
     6901 taacagctgc ccatgctcct ccacttgttc caattaagcc aactttacca caattcaaat
     6961 gttcaatcag tgctattgtc tgtaaggctt catcatgcca tatgtcagga gaaaattgag
     7021 aaactctatc cgactgtcca ttacctaaaa aatcaattaa gatacatcta aaattttcta
     7081 catataatgg cacaagtgct ttaaacatat tagaagaacc agtattacca tgcaggaaaa
     7141 ttatcggttt accttgtcca tattctttat aaaaacaaga attaccttga tactgaaaat
     7201 atgacatata ttttcctcct ttttatttgt taagttaatt ataacaatat aggtatgaat
     7261 gaaaaacctc atgttctttc ttagaataaa aatgtgccac gcataaaacg tgacacattt
     7321 ctaattataa attttgctgt ttaagtattc tttgtatgct tttctctgtc aaaaagtatt
     7381 tctctgataa ctgcttgata ctcattccat tttggaaatt tatacagatt tgagaattac
     7441 gagaagctaa gacttgtttg gtgtttgtgt tttcacccca atgctttttg tgctcttgct
     7501 ttttgggaat ataaatcact ttaccatcta tatattcttg cacttgctca atcagttctt
     7561 taggcagtaa gtcaatcgaa ttttgatagc tcattttgca cctcctaaat aattattctt
     7621 taggattagc aaaggctact gccactcaca attaaaaata aaatttttta ttatgtacaa
     7681 tagcctttgc tatgcacaag ttccaattcc cataaactaa atactccttt ctggtttgaa
     7741 aaattcatct gaacattgat ttcgctttta agatagagtt caatgacaaa tttacatttt
     7801 tattatatat attcagatag ataattgcaa ctgtctttgt atgaggatat tttattatag
     7861 tcaatactat aatatgtatt tatatcatca aactaatatg aacagttgaa aggaatatga
     7921 acaataaaac ttagtcagga agaataatta tgaagcagac aattgagaaa tttgatttta
     7981 aagttttatt gaaaatttat tgacaaattt aacttgtaaa tattatacta aacttaataa
     8041 ataaagttta taaagtaagg tgttgtctat gaaaaatata gttggaaaac cgcaaacgat
     8101 aaaaaaagta aatgaagatt taataaaaaa cattataaaa aatgaaggtc ctataacaaa
     8161 accagaaatt tcaagcatta ctgaattgag tttagctaca gttaataaga ttgtagagca
     8221 attagctttg aaaaatgaag ttaaagtaag tggactaagt gaatctactg gaggaagaag
     8281 agctcaatta tttgaaataa atgcaaactt agaacacata atagctttat attattatag
     8341 aaattgttgt ataggggtag ttgccaattt attaggtgaa ataatttatc aagaagaatt
     8401 taatataaga atggatacat atgaaaacgt taaccaagat actttttttg ttatagatac
     8461 tctaataaaa aaggaaaaag gaaacaatat aagggctatt ggaattggtg tcccaggagt
     8521 tgtaaaaaaa ggtgtaatta aaaatatacc aactataaaa agttgggaag gtataaattt
     8581 agaaaattta ataaagagta aatacaatac taaagtgttt atagaaaatg atacaaatat
     8641 tactactatt gggatttatc aaaaacagta taaagacaaa tataaaaata tggctcttat
     8701 gtatttagaa gatggtattg gttcaggtat agttataaat ggtgaattgt atttaggttc
     8761 tacaaatttt gctggagaat taagttatct aaatatagaa tttggtaagt ataactctaa
     8821 caacttaaat cttgaagatt atattatgag tttaagaaaa agatatttag aaagtaaaga
     8881 taataaattt aagaaagaaa ttttatctat aatatcaaac tttataagga ctcttgtatg
     8941 tgtcctaaat ccacaagcta tgattataca gtgtagtata ttaaccaaga gtgacatgga
     9001 gtttatagaa aaagatgtaa gcaataatgt tggagaagaa aattttccta aattaataaa
     9061 agtagagtca ttgagagaag gaagtataaa tggagtaatt agtatgtgtt taaaagaaac
     9121 tgactatcaa tattccttat ctaataaaaa aggaggatat taatgtgaaa tatttggttt
     9181 caatagatgg agggggaaca aaaactaaat tttgtgtaag tgacttagat ggaaacatac
     9241 ttaaagaaca taccactggt tcaactaatt ataagtcagt tggaattaaa aaaacatatg
     9301 aaaatattaa taatggattt aaaaaaatat taaaagattt atatatcgat tatgacgata
     9361 ttgagtacac tgtatttggt atatctggtt gtgattctcc taatgattat aagattataa
     9421 tagatgaaat attaaaaatt gggattaaca aagaaaaaat atatctggca aatgatgcag
     9481 ttttagcttt ttatgcacaa gctgattccc ctggtttagt aattgtggct gggacaggtt
     9541 caattatact gggaattaaa gaagatggag aaatatatag agttggtgga tgggggtata
     9601 attttagtga tttgggttct ggatatgata tagggagaaa attattaaag aaagtattgc
     9661 tttattgtga tgaatgtcat gagtatagtg atttatttag ttgtgtcttg gatttctttg
     9721 gagcaaatag ttttgaacaa cttgcatata tgattactga tataaacgat aatgttgaaa
     9781 tagcaaactt agcttcatta gtgattgatt gtgccgaaca aggtgataaa ttggctattg
     9841 aaatattaag agaatcatca actgaactat ctaaattagc tcaagtaata ttgagaaaaa
     9901 tatctaatcc aatgaaaatg gataaaagcg aaataaatat agttttgtct ggtggtacat
     9961 taaataaaac tgtatatgct caaatgttaa tgaataattt aaaaacagaa aatacagata
    10021 aaaatttaaa atttatatct caagaaaatc agccagtata tggggggata agacttgcta
    10081 tggcattagc taataggagg gtcaagcatg gataaaaaaa aggtaatagt acaaattttt
    10141 actggaggtt ttaaggctaa agaaataaag tttaatgata taaaatgtaa actactagaa
    10201 ttattggact gtattgatat agaaaaggtt atcatgggat ggagtgtaga tgaagaatta
    10261 tatgtagaaa ctaaaaaact attaaataaa tataatgttg aactatatct ttggttgcca
    10321 tgtttttctg aaataggatt acttgaaaaa agtaatttac taattgatta tatggggaag
    10381 gaagtcaagg gctatgcttt acaagaggat gaaaattttg agttttattg cccaaataat
    10441 aaggaaaata taaaaaatat aaagaaaata tatagtactt attttagtga tattaaattt
    10501 gatggaattt ttttagataa aataaggtat ggttcttttt cgaataaact aaatggtgtt
    10561 tttaattgtt tttgtaaaga ttgtttaaat atatatgaaa aaaataatat tgataaagag
    10621 tcgttaatga gagaaatgaa taatgtgtct aagggagtta tagataatga agatactcca
    10681 tttggtataa cagaatacat taatggaaaa tacaaattta aaaataaaat atgggaagat
    10741 ttttttgaaa caaaaaatca aaatatatac aaggcattat tagagataag taattatttt
    10801 aaaagtaagg gattgaaaat aggtatagat actttttctc cttttatttc ttactttaca
    10861 ggtcaagatt tagaaaaatt acaagaagta gcagatttta taaaaccaat gatgtataga
    10921 actacaaaag ctccagctgg tttaccattt gaatttgaaa ggtttattga tgaatccata
    10981 cataagaata aagagattgg aaaaagaaat ttcttagata ttttaggaat gaaggagatg
    11041 tatatcctat agaatttgta ctagaggaaa tcgattttat gacaagaaat cttaataaag
    11101 atatatattt aggagttgaa ataaataaaa aggaaattat agctcctgtt acacctaagt
    11161 acataagtga aaatatttca aatataaata agacaacagt aaaaggatat gttttatctt
    11221 gggatttgtt gtcagcacca aaagaaaata ttgatgaaat tataaaatgg tttcggaatt
    11281 agtgaggtga taaagtgggt aagatattgg aaatgcaaca aatatataaa agttttggaa
    11341 aggtagatgt tttaaaaggg ataaattttg agttagaaaa gggagaagta agagctcttt
    11401 tgggagctaa tggagcagga aaatctacac ttattaaaat tttaggagga gttcattctc
    11461 aaactaaagg taaagtaatt ttgactggta atgaaataat gtttaaaaat ccacatcatt
    11521 ctaaagaaaa tggtattagt ataatttatc aagaattaag cttgatacca acgcttacag
    11581 tacttgaaaa tatttttctt ggtagagaaa ttttaaaagg aagtgtgttt ttaaacaaga
    11641 aagacatgct tgaggaatat atgaaaattt gtgaggattt tgattttaat atagacccac
    11701 atatagtagt ttctaattta agtgtagcaa accaacaaat ggttgagata atgaaagctg
    11761 tttcatgtaa ttctgaaatt atcataatgg atgaacctac aacatcactt actaataatg
    11821 aaaaagatgg gctatttaaa ataataagta gactaaagtc taaaggtaaa acaatagtct
    11881 atatttctca catattagag gaagttttta aagtatgtga tagtgcaagt ataatgaaaa
    11941 atggaattat gattggaact tatgatgtag aggatttaac taaattaaag ataactcaac
    12001 ttatgacagg aaatgataat cacaaactta ttgagaagaa ggattataca tttgcaaagt
    12061 atgaagagac accagtacta gaagtaaaga atataagttg gtctaacaaa ataaaaaatg
    12121 tttcatttaa agtttataga ggagaagttg taggtcttgc aggtcttgta ggttcaagaa
    12181 gaactgaaat tataaatttg atatttggta tagataataa agatagtgga gacattttta
    12241 tcaatggtaa aaaagtagac ataacttctc ctaaggttgc tataaaaaac aaaataggat
    12301 tgattcctga agatagaaaa aatctaggat taattttaga acaagctata tacacaaact
    12361 catcatcagt acaaattgat aaattcaaga aattcttgtt tatagataga aataaagaaa
    12421 ttgagttttc taaaaaaggt gttaaggaat taggaataaa agttagtgat gtaaagcaag
    12481 aagtaaaaga gcttagtggt ggaaatcaac aaaaagtagt agtttctaag tggttagaga
    12541 aagatttaga tttaataata tatgatgagc cgacaaaggg aatagatatt gctgcaaaag
    12601 aagatatttt caatacaatc aaagaatttt caagtaaagg aataggagta atatttatat
    12661 cttctgattt agaagaagta ataagagtaa gtgatagaat tttagtaact agagaaggtt
    12721 ctattgtaga tgatttaaaa aatgagaata tacaggttca agatataatg aacagtatat
    12781 ttaatgtata aaaaatgaaa atggaggact gagatgaaga aagcttttga gaagttaaat
    12841 ttaagagatt atggagtatt attaggtttt ttagctttat gtatagctat aagtattgca
    12901 actccatcat ttttaggtaa acaaaatata cttaatcttt tacgacaatc ttctataatt
    12961 ggaataattt cagcaggtat gacttttgtt ataatatctg gaaactttga tatatcagtt
    13021 ggagcagtag cagctcttgc aggtgctata acaatgaaat ttgcgactgg tggaactaat
    13081 ttgtttgcat ccatgctctt aggtgtagcg atttgtgcag taataggatt aatcaatggt
    13141 atagtagttg caaagataaa tgtaccatca ttaatagcca ctatggctat ggtcactatt
    13201 gtaagaggat tgctgttgat gcttacagga ggatatccaa ttactgaaaa tataccaata
    13261 cttgattaca ttggaaatgg atatttgttt aatataccaa taccagtagt aattttcttt
    13321 atagtggtgt tagtttcgtt tattgtactt aataaaacta agtttggtag atatgtttat
    13381 tctgtaggag ggaatcaaga agcaagtaaa cttaatggta taaatgtaga ttctcacaaa
    13441 gtgaaagtgt ttattattaa tgctgtactt gcaggaatag ctggaatcgt actaacagga
    13501 agacttggga ctgcaactgc gattgcaggt gaaggatatg atatggatgc aattgcatct
    13561 gtagtaatag gaggtacatc agtggcagga ggttctggtt ctgtacttaa aactgttata
    13621 ggagtattac ttatgagtgt tataaacaac agttttaatc ttcttggaat agatgtatat
    13681 ttccagtaca tatttaaagg attaataatt ttagcagcag ttggatttga ttcttatagc
    13741 aagaaaaaac ttgcttcaag ataaaaaata agggggattt ttttatgaaa aaattaatga
    13801 aacatttagc attgttgcta tcacttgttt tgatatttgg tttagttggt tgttcaaatg
    13861 gtggtgacaa agaaaaaaat ggagaaaaga aaatagcagt attactacca ggttcaacag
    13921 gttactttgt agcaactaaa caaggtatag atgcaaaatc taaagaatta ggtgtaagcg
    13981 ttgaatatgc agatgctcaa tgggatgcta gtaaacaatt atcacaagct gaagatttta
    14041 tggctaaagg ggtagacatg attgcattgt gtggagttga ttctgctgta agtgagagaa
    14101 tagttaaagc tgctaatgat tcagacgttc ctatagtagc atttacaaat tctataggaa
    14161 gtaacccaac tggagaattt aaaggtttag ttacatatat aggacaaaat gaggaagaaa
    14221 caggagcatt aactggaaaa attgctaaaa acttattagg agaaactggt gggaaagctg
    14281 tattaataga aggagtacca ggaactactc cacaagtatt aagaaaaaaa ggtcttgaaa
    14341 aagaattaaa agatagtaat atagaaatag tttataatca aacttcaaat tgggagaaag
    14401 agcaagcttt aaaagtaaca gaagatttaa ttcaaaagaa aactgatttt aatgttataa
    14461 tatgtcaaga tgataattct gctacaggtg caggtcaagc tttaaaagat gctggtttta
    14521 aggataaagt taaagtcata ggtttgggtg gaagtaaaga tggattaaaa gctataactg
    14581 atgggttaat agatggtaca acttatatgt ctgctgtaga agaaggtgga ttagtcatag
    14641 aaaaggcatc taaattcctt aaaggtgaaa aaatagaacc tgttacacaa attaaacaag
    14701 ttgaagttaa caaagataat atttcagagt tcaagggtga atggtaaatt gtcatagata
    14761 ataaataaga tttttagagt tactttagaa actatataaa gtaactcttt ttttaattta
    14821 atttagtgat aaattattga atagtatttc taaacaaata aaaaattaat ataaacaaag
    14881 acttaattat actaaattat aagtaaaact aatataaact tatggaaaaa aggaaaacgt
    14941 ctaaagtatt ggaattacta tagtgtaggt gtggtattaa aaaaacataa aaaaacataa
    15001 aaaaattgtt gaaaaaaata tagaaatttt gtataattaa agatttgaaa agtgcggtat
    15061 acaaagccaa ttcttttgta tataatatat ataaattcag aaaaaggaag ggatatgtat
    15121 ggccaggcgg ttatttttag aaaggattgg acttatattt ttaggttcag caatattagc
    15181 atttggagtt tataattttt attatttaaa caacataaca gaaggtggag tgttaggtat
    15241 tttactactg ttaaaaaatt tattcaatat acaaccagca atagcaaatg ttgtaataga
    15301 tggtttactc cttttagtag gatataaatt tttcggtaaa aaattcctta tatattcaat
    15361 agtagcctct ataacttttt cagtgttata tgacttattt gaagctatag gacctctagt
    15421 accacaatca caaaatatgc ttttatcaac tattttggca ggtgtaactg ttggaactgg
    15481 tgtaggtata gtagtaaaag caggttgtgc atcaggagga gacgatgctt tagcattagt
    15541 aatttctaag actacatctt tgaacatagg gcaagtttat ttagcaacag atgttattgt
    15601 attgttgtta tcattatttt atctatcagc gtttgatatc ttttattctt tgattgctgt
    15661 tacaattagt ggcaaggtaa ttgattttat ttattatcat ggtaaaagtt tggatatgga
    15721 tataagtaat gatatagttc cagaatgtta atataaatta aataacttta gattttatta
    15781 ggggtggcag atttgccacc ttttttgtgt ctaaatcttt atagaaagta taatcccatc
    15841 taggtttttg caaatctcaa atatagaatt atgttggaat gactgggaat acgattgcta
    15901 gatgtgcatt ttaaaaaccc aattttatat ttatgaaata ctaatcatta atggatgttt
    15961 cttaatccta tctttatacc taaaatgata agataaatga agtagcaata caaaactaaa
    16021 atttgtaaaa gagattaaat taaatggagg agctaaaatg ttggatatag ttatgatagc
    16081 attgttattc ataggatttg tttccatgaa gttacttgct gattggtgtg ggaaacaaac
    16141 tgaaaaaaaa taatgtaatg tatggaggga ttacctatgt ggtttctggc agttgtaatt
    16201 ataatgttaa taatatattt ggtatatgcg cttattaatc ctgaaaaatt ttaaacagta
    16261 taaggctaat aagtaagttg aattgggggg aacaattttt atggtgcaga tagtaatagt
    16321 gttagcgatt tttatgattt tagtaatacc tatgggcaag tatttatatc atatagctac
    16381 taatcaaaaa acatttggag atagactatt tgataaagtg gacaatttta tatataaggt
    16441 atgtagtata gataagaaaa aagaaatgaa ctggaaacag tatgcattag ctttattatt
    16501 tacaaatgca gttatggtat ttataggata tatcatactt agaactcaaa gtatgcatat
    16561 ttttaatcca agtggaatta aaagtatgga acaaggacta tcatttaata caataataag
    16621 ttttatgaca aatacaaatc ttcaacatta ttcaggtgaa tcaggacttt catactttag
    16681 tcagatgaca gtgataatat atatgatgtt tacatcagct gcaacaggat atgcagctgc
    16741 tatggcattt gtaagaggtc tagtcggtaa aaagaaaaca ttgggaaatt tttatgtaga
    16801 tttaattagg ataacaacaa gagtactttt accaggagct ttaattattg ggcttatatt
    16861 agttacacaa ggagtaccac aaacttttgc tgggacagaa acagtgacta ctatagaagg
    16921 aaagcttcaa gatatagcta gaggtcctgt ggcagctctt gaatcaataa aacatttagg
    16981 aacaaatggt ggtggattct ttggttcaaa ttcatcacac ccatttgaaa atccaacaat
    17041 aatatcaaat atagtagaaa tattatctat gatgatatta ccaggagctt gtgttgttgc
    17101 attcggtcac atgataaaga ataaaaaaca aggttgggtt gtgtttggcg caatgtcaat
    17161 catattttta ataggtctag tagtgtgctt taaagcagaa agtgcaggaa accctatttt
    17221 atcacaatta gggcttaatc aaagtatggg aagtatggaa ggtaaggaag ttagatttgg
    17281 tatagcacaa tcatcattat tcacaacagt gactacttcc tttacgacag gtacagttaa
    17341 taatatgcat gatacactga ctcctttagg aggattagta ccacttttaa atatgatgct
    17401 aaatgtagta tttggtggta aaggcgtagg tctgatgaat atgttgatgt acgctataat
    17461 agcagtattc ttatgtggat taatggtagg aagaacacca gagtttttaa ctaagaaaat
    17521 cgaaggaaaa gaaatgaagc ttattgcttt acttattata ttacatccac ttttaatact
    17581 aatgttctct ggtctttcag tagcaatacc agcagggctt gaaggaattt caaatcctgg
    17641 attccatgga ttatcacagg tattgtatga gtttgcatct tcagcagcta ataatggttc
    17701 aggatttgaa ggtctaggag ataacactat gttttggaat ataacaacag gtatagttat
    17761 gttctttgga agatatgtgt ctattatagt actactagca atatcaagtt tgttagcatc
    17821 taaaaaagct gtaaatgaaa gtataggaac acttagaacg gataatttta catttacaat
    17881 agttttagta cttgttgttt taattgttgg tgctttaaca tttttcccag ccttagcgct
    17941 tggacctatt tcggagcatc tagtgttgtg gcactaaaaa attgggaggg agtaaagttt
    18001 tatggaaact aaaaaaacaa agtttattac aaaagatatt ttcaaaactt ccattataga
    18061 agcttttaaa aaattaaatc ctaaatatat gatgaaaaat ccagttatgt ttgttgtaga
    18121 aattggattt atagtaacat tgatacttac aatagtacct agtttgtttg gtgatgaagg
    18181 caataactta agagcttata atgggattgt aacaataatc ttatttataa cagtcctttt
    18241 tgcaaatttt gctgaagctg tagctgaggg aagaggtaaa gctcaagcag atacacttaa
    18301 gaaaactaga aaagatacag tagcaagggt tatagatacg ttaggaaatg aaaaaatgat
    18361 aagtgcaagt gagctaaaac ttggtgatat tgttttagta aatgctggag aagttattcc
    18421 aaatgatgga gaagttatag agggtatagc ttctgtggat gagtcagcaa taactggaga
    18481 atctgcacct gtcatgagag aatcaggtgg agatttcgca tctgttacag gtggtactac
    18541 agttgtaagt gactggttaa agataagaat aacaacaaaa ccaggagaat cgttcttaga
    18601 taaaatgata gctcttgtag aaggtgcttc aagacaaaaa acgccaaatg agattgcttt
    18661 aaatactttg ttagtagggt taacacttat atttatggtt gttttaatat ctctttatcc
    18721 aatggcaaac tatgcaggtg taaagattcc aatttctact atgatagctc ttttggtatg
    18781 tttaatacca actactatag gtggattgtt gtcagctatt ggtattgcag gtatggatag
    18841 agtaactaga tttaatgtaa ttgcaatgtc aggaaaagca gtagaagctt gtggagacgt
    18901 tgatactatg attttggata agacaggtac gattacttat gggaatagac ttgcatcaga
    18961 atttatagca gttggaaatg cagattcaaa agatttaata aattattctg ttatgtgttc
    19021 attaaaagat gatacaccag aaggaaaatc tatagttgat ttaggtatga aattaggttc
    19081 aacaggtaag acaaaagagg cagaggaagc tgagtttgta gaattttctg cacaaactag
    19141 aatgagtggt attaacttag ctgatggtag agctataaga aaaggtgctt atgatagcat
    19201 aataaaaaga gtaaaagaat ctggtggtac agtgccagaa gatttagaag aaaatgtaaa
    19261 tcgagtagct aaactaggag gaacaccttt agtagtgtgt gtagataatg agatatatgg
    19321 aattatctac ttaaaagata cagtaaaatc agggcttgta gaaagatttg ctagacttcg
    19381 agagatagga ataaagacaa taatgtgtac aggggataat cccttgacag cagctacaat
    19441 tgcaagagaa gctggagtag atggatttat tgcagaatgt aaaccagaag ataaaataga
    19501 tgcaataaaa aaagaacaat tagagggtaa aatagttgca atgacaggtg atggaacaaa
    19561 tgatgctcct gcacttgctc aggcagatgt aggtcttgca atgaatagtg gtacaacatc
    19621 tgcaaaagaa gcagcaaata tggtagattt agattcagac ccaactaaga tattggaagt
    19681 tgttgaaata ggaaaacagc ttttaataac tcgtggggct ttgacaacat ttagtatagc
    19741 aaatgatgtt gcaaaatatt ttgcgattat accagctata tttactcttg caatacctca
    19801 aatggagata ttaaatgtaa tgaaactttc tacgtcattt agtgcgatat tatcagcttt
    19861 aatatttaat gctataatta tcccatgttt aatacctctt gcaatgaagg gagtaaaata
    19921 tagacctatg aaatcacaag ctatgttaat gagaaatatg cttatatttg gtcttggtgg
    19981 agttatagta ccatttttag gaataaaatt aattgattta ttgattacac ctttagtaat
    20041 ggttcttaat ttaggttaat aaatggagga tataaaaatg aaaacattaa agccagcatt
    20101 acttgtaagt attgtattgt tagttgtttg tggacttgta tatccacttg ttttgacagg
    20161 tgtaagtcaa gttgcattta aagataaagc taatggaagt atgatagaag taaatggtgt
    20221 aaaagtaggt tcagaactta ttggtcaaag ctttacagat gcgagattct ttaagggaag
    20281 agtatcatca gtaaactata atacttatac aaaagaagat ttagtaccag ataaagatgg
    20341 aaatacatct tatggaggtg tatcatcagg gtcttttaat tatggagcta caaatccaga
    20401 acttcatgat agagtacaaa aagatataga acagttttta aaggataatc caactgtcaa
    20461 aagagaggat ataccaacag atttacttac agcatcaggt tcaggacttg accctaatat
    20521 aagtccagcc tctgcaaaaa ttcagatacc agctatagca aaagcgtctg gaatatcaga
    20581 aagtaaatta caaaaaattg tagatgataa tacttccaaa aaactttttg gagttttagg
    20641 tgaagataga gttaatgtac taaaggttaa tgtagaggtc gcaaaaatat taggtcttat
    20701 atagtatttt gtagaatttt agtaacaaaa tattatgcaa gatttaattt aaaggttaaa
    20761 ttttaaagag tgaaaatcaa taatgatttt cactttttta tttgaatatt actaatattt
    20821 ggaataaagt gattgtaagt ataaaatcca aaaaaaggtt gcattatcaa aatgataata
    20881 ctataatata tttatcgtta tcaatttgat aatagatttg gacatatagg aggaaatagt
    20941 atgttatata ataacgcatt agagttgata ggaaacacac ctgtagtaag gctgaataat
    21001 ttaggctgta ctaatgggtg ttctaacata tatgtaaaat tagaaaaagt aaatccagca
    21061 ggaagtataa aagatagagc tgtttatgaa atgttagagg gcttagagca aagaggagaa
    21121 ttaaaaaagg gagatgtact agttgaagca actagtggaa atactggtat tgcattatct
    21181 atgataggaa aattaaaagg atatgaagta gtaatcgtaa tgcccgaaac aatgagcgtt
    21241 gagagaagag atttaatgaa ggcttatgga gcaaatttgg tgcttacaga tggtaaagct
    21301 ggaatggcag gttccataga aaaagcaaat gaacttttaa aagaaaatcc aaactataaa
    21361 agcttaaaac aatttgaaaa taaagataat ccaaatgcac attataaaac aacagcagtt
    21421 gaaattatga atgatgtcaa agatttagat atattcgtat gtggagttgg aacaggtggt
    21481 actttaatag gaactgcaag atatttaaaa gaacaaaatc caaatataag agtaattgca
    21541 cttgaaccaa aaaaatctcc agctatatca gaaaacaaga ctggacctca taaaatacaa
    21601 ggaataggtg ctaactttgt tcctgaaaat tatgattcta gtgtagttga tgaagtaata
    21661 ttagtagatg atgaagatgc atttgataca gttaaattac ttgcagcaaa agagggaatt
    21721 ttagtaggga tatcttctgg agcaaatata tttggtgcta tagagatggc aaaaaagtat
    21781 ccagataaaa agatagttac agtagctcca gatggagttg ataagtatat gtctatggga
    21841 atattttaat aattttattt gagggagtga atactttgtt taagaaaata aataaagata
    21901 ttgaatatat aatgaagaat gatccagcag ctaggtctaa gatagaagtt tttttacttt
    21961 atccatcagt tcatgcaatg ataatgcata ggatggctca tgctctttat aagaagaaga
    22021 agctttttac agcaagatta atatcccaga tatccagatt tatgactggc atagaaatac
    22081 atcctggtgc taaaatgggg gaaggtatat tgatagacca tggaatgggt gttgtaattg
    22141 gagagacagc ggaagtaggt aatagggtga caatatatca aggagctact ttaggagcta
    22201 caggaaaaga tactggaaag agacatccaa ctgttggtga tgatgtatta attggtgctg
    22261 gaacaaaaat actaggacct ttaaatatag gttcaaattc aaaaattggt gcaaattctg
    22321 ttgtcgttaa ggatgtacct aatggtgcaa cagttgttgg tataccagca aaaatagtaa
    22381 aaataagaaa cctagaacct gtaaaaaaga ataaaaaaga agtgtcctat gagtatgatg
    22441 aattagataa tgtatattat atataggatt tatataacta taaaataaac tttataaaaa
    22501 ttattttaaa aggtattttt ttcaaatttg aggacataat aaaaaagaac tgaatgttac
    22561 agttttggtt catcaatctt tgataaacaa gattttaaat ttgtagcgtc taggaggatt
    22621 ttattatgaa agcaaatgtt aatcaagata cttgtatagg ttgtggatta tgtccatcaa
    22681 tatgtccaga agtatttgat atgaaggatg atgggaaatc acatgttata gttgatgaag
    22741 taccaagtga tgctgaagaa tcagcagcag aagctagaga aagctgtcca gttgatgcaa
    22801 tagatgtgca ttaataaaat aaatgcctat aaaaattgaa ttttcaattt ttataggcat
    22861 ttgtctttta ttcagttagt atttcaacac ctgtttctgt aactaaaacc atacattccc
    22921 attgagcaga taatttgcca tcttttgtat aaatagtcca attattttct tcatcaacaa
    22981 aaatttcgtg agttccttca tttaccatag gttctattgt gaatatcatc ccaggaacca
    23041 acagcatctc agtccctttt ttagatacat aacttacaaa agggtcttca tgaaagctta
    23101 atccaactcc atgaccacct atttctctaa caacagagta tccatttttc tttgcataag
    23161 agttgatagc atcacctata tctcctaaaa atccccatgg tttggcaact tcaagacctt
    23221 tttggacaca ttcctttgta acttgaacta atttttttgc attttcatta acattaccta
    23281 tcataaacat tcttgaagca tctgaatagt atccattaaa aatagtagaa acatctacat
    23341 ttataatatc tccatctttt aagataatgt ctttacttgg aataccatga caaacttcat
    23401 tatttactga tgtacataca cttttaggaa atccattata gtctagtgga gcagggatag
    23461 ctccattttg tagtgtaaag tcatgaacca acttatttat atcttctgta gacattccaa
    23521 tttcgatttt atctgaaata taatccaaaa tttttgtgtt tattacagca ctttttttta
    23581 tttcctcaat ttgactttca ttttttataa tatcatgagt tggtacaata tgacctaact
    23641 tagcaaatga atctatttta ttatccatat ccatatgaca ttttttatat tttttagtac
    23701 ttccacacca acagctatca tttctattta acttcataac ttttcatcct tcttttatta
    23761 tttatttaaa ttatgggtta cataaactat tttaaaacat aaccattata aatattataa
    23821 cataaatact aagaaatcct ttatagatag tgatttattc aaagtataaa ttattatacg
    23881 gttattgttg atgaaaagag gatagttaaa tgtattattg gatattagcg cacttaatag
    23941 tttagttcaa gtttttcaag aaaatcatat gttagaggac aattttcctt taaagtattt
    24001 tttgaatctt catcaagata ataatgcaca aatgattctg caaaaaattc ttctataaac
    24061 ttgcttatat atgcatgtgc aacttgctta gggtcataaa gcttatatct ttcctgagca
    24121 aatacttcca taaattcttc agaatttgat tttttattta aaacattttt atctatagca
    24181 tgtgcagttt catgtaattc taaatttttt gatgaatgta catttgcata gctagggtta
    24241 ctataaccta ttttagccac tatagaatta tttctatata taccaggtac atctttccaa
    24301 gtcttgcctg atcttaccca acatggaggt aattgatatt ttaaatcact aaattctggt
    24361 tcatcagtta gatttgaatt tattaatttt atttttatat ccttttcttt aagtgtctgt
    24421 aaatatttaa tatttatttt tgcaagtctg tttttcatat tttcaacttc atctttatca
    24481 taattcaagt catcaggaag tataagcatt tgttcaatta tatctatact actgtctttt
    24541 atattttcag attcttcatt aaatttatat tgaagagtat tattttcata gatagttata
    24601 tttgtattat tttctataga ataagagtga atatcctcta aatctaaaac attcatttgt
    24661 tgtaaaatat gttttgtttc gccactccat actactaaag aaagtgaatt aaaattaatt
    24721 agtggatttt cttttgcttt tatatcattg cttatatcta catcaaatac atattcaatt
    24781 gagtcttcta tgttaagact atctgtattc tttttaatat ataaatacgc ttgttttaag
    24841 tctatataaa tctcatctaa agattcgtca taaaattctg ataaattatt agtaaagagt
    24901 tcttcactta aaagttcatt tttatcagtt atactttcaa ttaaaatatt aatttctttt
    24961 atcatatcat aaatacttat ttttctattt gctttttcac tagatacatt gcatggcgca
    25021 agagcaatgc aataaaaatc tttttctttt ttataaaagg ctttaagtga ttttgataaa
    25081 agtgtaaagt ctacacctat ctgtaagttg accttaagtg agtcattttg taataattca
    25141 gtattatttc ttttagctgt atttaaattg ttttccataa tcattgcccc ctttttatta
    25201 gggtatcata atttttttat atgtgtcaat ttattttttt gaaaattata atttttttga
    25261 aaattttatt ttttataatt tgtgaatttt ttattataga aataaatatg ttattattat
    25321 gtcaaaattt agcattagat tcatgtagag tatgttttat acatatatta taattaattc
    25381 tttgaatat
//
LOCUS       DAEMHW010000043        24361 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEMHW010000043 DAEMHW010000000
VERSION     DAEMHW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.043"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            23..769
                     /locus_tag="KPK67_001484"
     CDS             23..769
                     /locus_tag="KPK67_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBG2458475.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHVELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            830..1504
                     /locus_tag="KPK67_001485"
     CDS             830..1504
                     /locus_tag="KPK67_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG2458476.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISEYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTDEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            1872..3150
                     /locus_tag="KPK67_001486"
                     /pseudo
     CDS             1872..3150
                     /locus_tag="KPK67_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
     gene            3177..4577
                     /locus_tag="KPK67_001487"
     CDS             3177..4577
                     /locus_tag="KPK67_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG2458477.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALKTKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSNINGIGIS
                     DEDYGDKVKIKKGNIYINSNDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            4776..6212
                     /locus_tag="KPK67_001488"
     CDS             4776..6212
                     /locus_tag="KPK67_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG2458478.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGKETVYYYKHHEPDKTNQIQ"
     gene            6556..7500
                     /locus_tag="KPK67_001489"
     CDS             6556..7500
                     /locus_tag="KPK67_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBG2458479.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7669..8601
                     /locus_tag="KPK67_001490"
     CDS             7669..8601
                     /locus_tag="KPK67_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBG2458480.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8706..9173
                     /locus_tag="KPK67_001491"
     CDS             8706..9173
                     /locus_tag="KPK67_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBG2458481.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9417..10271)
                     /locus_tag="KPK67_001492"
     CDS             complement(9417..10271)
                     /locus_tag="KPK67_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBG2458482.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10437..12401
                     /locus_tag="KPK67_001493"
     CDS             10437..12401
                     /locus_tag="KPK67_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2458483.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSCCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAINHITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVAKLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            12401..12862
                     /locus_tag="KPK67_001494"
     CDS             12401..12862
                     /locus_tag="KPK67_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2458484.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            12865..13185
                     /locus_tag="KPK67_001495"
     CDS             12865..13185
                     /locus_tag="KPK67_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG2458485.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13201..14301
                     /locus_tag="KPK67_001496"
     CDS             13201..14301
                     /locus_tag="KPK67_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG2458486.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14421..17021
                     /locus_tag="KPK67_001497"
     CDS             14421..17021
                     /locus_tag="KPK67_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBG2458487.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INIQLPALGYKVVSFESSDKEMVSLDKSNDTFISNHNYKIIYEDGSLNLQLKDGTYIS
                     NFLILKDSGNAGDTYDFSPLKNDEDIKLSFDRVYTEKALGYEKLVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTIFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
     gene            17491..17826
                     /locus_tag="KPK67_001498"
     CDS             17491..17826
                     /locus_tag="KPK67_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2458488.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            17963..19240
                     /locus_tag="KPK67_001499"
     CDS             17963..19240
                     /locus_tag="KPK67_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBG2458489.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            19361..20749
                     /locus_tag="KPK67_001500"
     CDS             19361..20749
                     /locus_tag="KPK67_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2458490.1"
                     /translation="MKKTFDAIIIGFGKGGKTLSGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTETEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQTKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            21120..21833
                     /locus_tag="KPK67_001501"
     CDS             21120..21833
                     /locus_tag="KPK67_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBG2458491.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHSKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            21951..22820
                     /locus_tag="KPK67_001502"
     CDS             21951..22820
                     /locus_tag="KPK67_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG2458492.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(22975..24120)
                     /locus_tag="KPK67_001503"
     CDS             complement(22975..24120)
                     /locus_tag="KPK67_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG2458493.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
BASE COUNT         9659 a         2549 c         4171 g         7982 t
ORIGIN      
        1 actgtgaaat ggaggtagta acatgaaaat tgaaaatctt tctattgatg atattaaacg
       61 aggctatata tttaatgatg atttaaattg ttatagttgc attaaatgta caaaaacata
      121 tgaggtagga gaaatatata aaataggtaa tagattcttt gaagcttcaa aagctattga
      181 acttcatgtg gaattagaac atagtgatta tttagagcaa ttgcttaata gtgattcaaa
      241 atacaacaca ttgactgaaa atcaaaaaca attatttcaa ttatttatta gtgatttacc
      301 agataaggag atagcaaaac aggttggagt atccacatct actatacgcc atcaaaaatt
      361 tattttcaga gaaaaagcaa agcaggctaa gctatattta gcactatatg aatctgtatt
      421 tgaagataaa tctaataaaa gcagtactat aataccaatc catgaacatg ctcgaatggt
      481 agatgaaaga tatgtagtaa ctgaagaaga gagaaaacac attttggata catcatttga
      541 aagtatgaat cctttaaaat taaaagtatt ttcttcaaaa gaaaagaaaa aggtcgtaat
      601 tttatctaag attttggaac aatttgaacg tgggaagaaa tattcagaaa aggaagttaa
      661 tcagatttta gaatctatat atgatgattt tgcaacgatt agaagatatc taattgagta
      721 tggttttatg agtagaaata aagaatgtac aaaatattgg ttaacataat taaaataata
      781 aattttacta atgtgtaatg ctatttttat ttagggggag gagtcaaata tgaatgatga
      841 attaaaatct attttataca ataaaggtgt agatattgtt cgctttgttg atatatctga
      901 atttccaata aatcaaacac aaggtttttc taaagcaatt ttattttgta tagggttatc
      961 aaaaaaattt attacagata tatacaataa cttacctaca gatagtgatg agtttttgga
     1021 aaaagaggag aaagtagaag aattagctga ctggatttca gaatatattc aaaataaagg
     1081 atatagagcg tattctcaat cggagaaaaa caatctagaa catggatatt ttgaaaaagc
     1141 ctatattaat ccagaaatgc aatcagggat tagtccttta ccacataaga caatagcaaa
     1201 tattagtggc attggattta tgggaaaaaa taatttgttt gtaacagacg aatatggatg
     1261 tgcttttagc atgtgtacag tacttacaga tgctccgatt tctgttgaaa gatatccttt
     1321 gattgattca aaatgtatgg attgcaatgt ttgtgtagag aactgtcctg caaaagctat
     1381 acatggaaat gaatggactt tacctggaaa aagagaatct attattgatg tttcaaagtg
     1441 tttttgcgta ttgaggtgta tgatgagttg cccatggtca ttaagatatg caaatcaaaa
     1501 ataactttac tagagaaggt attaaattga atattataaa atttttaaaa tgtaagatta
     1561 tataagattt ttcaaaataa aaatttgtat agaacactca gaaatgagtg tttttttatt
     1621 ggaattaaat tatattgata caaagcttaa ctattttaat cattatataa actttgtatt
     1681 aaaaaattaa gaataacttg ctaatagtaa aaatataatg agattataat tgatttataa
     1741 tataactgta atgatgttgt aaaatgaaaa ttgtcatctc aaaattccca aaaagagtaa
     1801 aagtcacttc ggttatagtc aaaaaatttt ttcagtgaaa agaatttaga tttaaaaggg
     1861 aggaattaat catggaaatt gaaactaaaa ctccagattg gattaatgta ctagaattaa
     1921 acagtaaaat agatattact gggaaatatg atgtaagtaa tatgatacaa gatattgtct
     1981 ctgataagtc actagaaggt tccattatat attttccaaa aggtaattat ttatttgaga
     2041 aaggtattaa aattagtcaa caaataacac ttcaaggaga ttcttattat ggaggagata
     2101 atcaagtatc aaatttagat aaaaaacaat caatcatagg aacaaccaat tttatatttt
     2161 tattatgata gtcataagat agaggaaata ttacctgaaa atgtatctgc tattacagaa
     2221 tatggagaaa cacaaggact atctcatttt gaacacctgt ttatttctgg attttcaggg
     2281 ataggaattg aaattccata ctattcaact ggcaatgata ttactgttag tttatgtggt
     2341 cttggtatga ggattggaga gaagtcggtg ctttctagta gcaagattta cgaatgtcaa
     2401 aatggaatgg agattactac tggtgttagt ttaaacaatg taagaatcga agaaataaga
     2461 gaaatcggta ttaataataa gggggttggt tttaacctga ttatgaatct tatggtcgac
     2521 caatgtggat attgtggatt tatgtttgaa aaaatgtact atagtcagat aactactaga
     2581 attactagat gcggtcagta ttataaaagt gctgattata atgcttatga gaatatggaa
     2641 gatagaaaag aagaagctta ttctattttt tatggagata ttttagaaaa ttgtaatatt
     2701 gtattgatga atagtaatat ggatacctta gaaagtgatt tacttaatga acataagata
     2761 tatgtaatca aggcaaatga aacaaacaat gtaatgctaa catgttatgc tgaagcagat
     2821 aactttatac aatgtgcaaa gggcaacttg ttgctagaaa atggtgaaaa tacttataaa
     2881 ttttataatg gtgagatttg ttctgtagga ggtgttcaaa tttctgatat agatgaaaaa
     2941 gatttaatca aaataatcga gaacactctg tatatagatg ataaaggtaa gtatttaatg
     3001 gttcctagat taaaaaagaa tactgtaata tattcagtac tagattctac agaagaaata
     3061 aataaattac atggtggaat atgggaacag attgatgtaa aagtattagc tggggaatcc
     3121 atgtactatt ataaaaagat aaaagattaa attatcaaat taggaggtag tgattaatgg
     3181 aagagtctat aataattaaa gatataagtg gctggattaa tgtccttgat tatggaattt
     3241 gtaatgatga aacaaaagat gtaagtttta agttgcaaga gattatggat gctgtaccag
     3301 aagattctat tatatatttt ccaaaaggaa aatacttatt tgaaagtggt gttaagatta
     3361 agaaacgatt aaccttatgt ggagattctt atattttagc gaatgttcct aaaaatactg
     3421 ctggagtaac acagtttact tttaaaggaa agaaaaatcc taaaagtgaa aatatagcaa
     3481 tcataacagc aaaaagtgta agacactgta ttaagaacat ttgcttttat tcagatagtt
     3541 gtgatagaga tatgacatct aatcatgaac ctccaacaga tggaaaacct agatttcatc
     3601 ataatgtaat tataaattat agaaatatat ctgctgttat atgtgaagat aaacagggag
     3661 aaccaggtca ttatgaaaat ctatctttta gtggtttttc tggaattgca cttgatatgc
     3721 ctaataattc tacagcaaat gatattacaa tttttacatc cggacttgga attagtacag
     3781 gggaaaattc aacaattgct aacagtaagg tttggggctg tgttgatgga atgataattt
     3841 ctactggaac ttttataaat ggtatgaggg tagaagaaat aaaaaaagtt ggaattaaaa
     3901 atattggtag aggtttaaat tttattacaa atatcacaat tgaccaatgt ggatattgtg
     3961 gagtttggtt tgattctata tccaatgttc agatatcagg aaatatcaca aggtgtgggc
     4021 agtactatta tgatgtaagc tatgataatt atttaatgtt ggataataga gttaaagaag
     4081 catattcttt attctatggt aatttattgg aatcaagtaa tatagtttta tcaaatacca
     4141 atgaagacaa ttgggaagat ttcaaagagg ataagcataa ggtgtatctt atagaagcac
     4201 ttaaaaccaa gaatgtacta ttaacatgta atatagatgc tactgactgg attgttaagt
     4261 cagaaaaagg aaatttaacg tacaataata tgcgacaaac tgttatattt tacgatggaa
     4321 aactaagtaa tatcaatgga attggtatat ctgatgaaga ttatggggat aaagtcaaga
     4381 taaaaaaagg aaatatatat ataaatagta atgataagga tattaagatg cataaaccag
     4441 aggtaggtga cattatgtca acaattatta aagataaaag tattctttct gctcattatg
     4501 ggggaacatg ggagaaaatt ggtgaaaaaa tagagtttgg aataactgta cactattata
     4561 aattgataga gaaataagta aatacgtagt atttgaatag atgtatttta ttggagagct
     4621 aggactatag ttttaatgta aatggtttgg taatagcatg gattgaataa tataaaaaat
     4681 taatttgatt atgtaaattt ctatgaagta gtaaaactgt gactttagtc ataggaggtt
     4741 caaactaagt aaaagggaaa ggaatatata atattatgga aggaagaact gggattaaag
     4801 atggaagatg gattaatgta gtagaaagag gtattaaaaa tgatggttcg tacaacgtag
     4861 gtcctgcttt gcaggatatt attgatgaaa tagaaacaga tttttgtgtc ttatattttc
     4921 caaaaggtaa atacctattt acaagtggtg ttaaaattaa taaagaaatt gtattgcaag
     4981 gtgattctaa ttcatctaca ggtactacac aatttataac tcgtggagta gaaaatatga
     5041 gtattattac attgacaggg aagaaacaat gtattaaaaa tataaatttc tattctgata
     5101 gttgtgagat acaggtaaat aaagaaccac cgacaaaagg taatcctaaa tatcattatg
     5161 agatgattat taataagaat gaaaagaatg ttgagatgaa taatgtatca gcaatcctgt
     5221 atgataacag taagaatatg attaaaggtc taggtcatta tgaaaatatt tatataagtg
     5281 gtttttcggg tacaggaatt aggataccat attactcaat tgtaaatgat attacagttt
     5341 catcttgtgg gctgggtatt gatacaggaa tagacacaat tatttctaat agtagaatat
     5401 ctaagtgtca aaatggaatg aggattagaa ctggaactag tataaataat gtaaggattg
     5461 aaaaaataca gaaagttggt cttataagta gtcaagttaa ggggtatgag gggtatggaa
     5521 gttataaaat taataatatt acgattgacc aatgtgggta ttgtggcttt tcatttgatt
     5581 caatgaaaga tagttatttt tctggtatta taagaaggtg tggtcagtat tattataata
     5641 cagattatga cacatattta agcattaaag atagagtaga agaagcatat tctttatttt
     5701 atggaaatta ttttacagaa tgtaatattg atttagttag tgataataag gataactggg
     5761 atgatggctt agaatataaa cataaagttt atgtatttaa aacccttcaa ataccaaaat
     5821 taacattgag atgcaatata gatgcctatg attatatcgt taaatcggta catggaggag
     5881 gtaatttaat gttaaaaaat aactttaaca attatatatt tattgattca tctagtccag
     5941 aagttattaa tggaattaat tttaattatc aaaagaatgg aaacttgtta aggttaaata
     6001 atggtaaatt aagttataaa aatggaaagg aagaaacagc tatattcaga cctaattatg
     6061 gtcttattgt agcatcaaca gattcagaat gtgaacaaat agagactagc tatggaggaa
     6121 aatgggagaa gcttggttct gaagtaattg gaaaagaaac ggtatattat tataaacatc
     6181 atgaaccaga taaaacaaac caaatacaat aaaatttgta ttatataatg atataaagtt
     6241 ttaatatata gtgtttacct ttaatgtaat attggtatat ttgtatagta ttaaatttgt
     6301 taaaaagtaa aattgtaggg tgcttgaaaa tagcaccttt tttagtttca ttataaaatt
     6361 agttttgaaa gtttaggaat aatgtatatt tatttttaaa gaattataac cttattaagg
     6421 atattttaaa tgctagaaat gaatcagtaa atcatactgg aattatctta aatgttttga
     6481 ttaaattaag tatttagagc tataattata ttaaaaatat attagaacat aattatatta
     6541 ctatggaggt taaatatgat agtaagtgtg agtagaagaa ctgatatacc tgctttctat
     6601 agtaagtggt tttttaacag acttaaagaa ggttttgttt atgtaattaa tccaatgaat
     6661 ccaaaacagg ttagtaagat agagttaaat ccacatactg ttgattgttt tgtattttgg
     6721 acaaaggatg taactccaat gttatatgat ttaaataaat taaaagacta taaatattat
     6781 tttcactata ctataacatc atatggaaaa gaagtagaga catgtatttt agataaaaga
     6841 aaggtaatag attcatttaa ggaattgtct agaaaaatag gcaaagaaag agtaatttta
     6901 agatacgacc cgattttctt aagtgaaaag tacacagtag attatcatat caaagcattt
     6961 gcgagtttat gtaatcagct tgatggattt acagaaaaat gtataataag ttttatcgat
     7021 ttatataaga aaacaaaatt taatactaaa gcattacata taaagccaat agaaattaca
     7081 gaaatagaaa cattatcaaa agagatttct aagattgctc ataagcatgg gataatactt
     7141 gaaatgtgtt ctgaagaata taatttaagt aaatttggga ttggaaaaag taagtgtatt
     7201 gatgatagat taatatcaaa aattatagga tgtgaagtag aggtaaaaaa agatagtaca
     7261 caaagagata tttgtggatg tgttaaaagt gtcgatatag gacaatacaa tacttgcaga
     7321 cattattgtc tgtattgtta tgctaacttt aattatgcaa aagtagaaga aaactgtagg
     7381 ctttataaag aagataataa attactcata gatactttaa gagatgatgc aaaaataagc
     7441 attagagata tgaagactat taaagtagat aagaataaac aaattagtat atttgattaa
     7501 aataaatata ccaattacct taatatatta agaatataga tacttgtaat aataaaatta
     7561 aacttaatag atattttcta tataagaaag atggtcataa tatgtcaatt tgaccatatt
     7621 tttgatataa ttaaaacaca aataatttag taacaaagga ggcagaactt gaatttatta
     7681 aaaaaatatc ctatacctat tgcaagtttg attttgtcaa tctttacttt aggtaactta
     7741 ttacaatctt atagtgaatc attacgtaat gtaattggat ttataggatt tattttgtat
     7801 gcaatttata tagtcaaaat aatattactt agaaagggtg ttaaagagca attagagaac
     7861 cctttaatat caagtacatt tccaactata acaatggcaa caatgatatt tgctacatat
     7921 ataaaaccat tatctttaga attaagtatt atgatttgga gcataggaat tgtgggtcat
     7981 cttgtgttaa tagtcttatt tagtaagaag ttcttagtta aattctcaat aaaaacggta
     8041 tttccatctt ggtatatagt atatgttggg atagttgcag ctagtgttac ggcaccagca
     8101 tttaatcaac ttttgattgg caaaattgct ttttggatag gatttgtatc ttatatagtt
     8161 cttataccag taattttaaa gagagtatgg ataatcaagg aaatgcctga accatcttta
     8221 ccaagtatag ctatattagc tgcaccaggg tcactattat tgtctggata tattaattct
     8281 ttttcagata aaaataatgt agtattatac ttgttattca tattatcaat tgtattttat
     8341 attatagttt tgggatattt accaaaatta ttaagattgc ctttttatcc gagttttgct
     8401 gcatttacat tccctatggc aataagtgca atgggtatga aactaatgaa tggttattta
     8461 actaaatcag gtcattttat aagttggatt tcatatgctt ctaagttaga agagataatt
     8521 gctacagtga ttattttata tgttttagtt ttgtttatta tatatcttag taaatctgaa
     8581 actacatcta aagctaaata agaattatta aatatgtgta tttaatagga atattgatat
     8641 aaatatatga agataagata aaccatagaa acttaattta gcttaatttt attggaggaa
     8701 aacaaatgaa aacaaaagaa gaatatttaa aagaagttag aaacatatca gaagaatact
     8761 ttagaaaagg agagtttttt tgctctgaag cagttctcca gacaataaat gatgctttag
     8821 gccaaccctt aagtccagag ataactaagt tagcatcagg atttccgata ggacttggaa
     8881 aggcacaatg tctttgtgga gcaatatctg ggggagagat ggctttaggt atagtatatg
     8941 gaagagttca tggtgaagct atgaatccaa agatgtttga gcatgctaaa ggtttgcatg
     9001 attatattaa aaaagagtat ggtgcaacat gttgtagagc tattacaaag aaatgggatg
     9061 gtgataactt tatgtctccc gaaagaaaag agcactgtat taaaataaca ggtcaagtta
     9121 cagagtgggt tgcagataag cttatagaag atgaaaaact taatatcaaa tagtgtaata
     9181 ttagttggtg aatttataaa gttaaattta cttatagtat aagatacttg atattctcgt
     9241 attgatagaa aaatttatag catatgtatt taatgaaata tacttatatt tagagtgtgg
     9301 tttaatttta taaatacata tgctattatt ttaagtatat aagcaaattt atttaaataa
     9361 atagaaacag ttaatttata tgtacaaaat tatatatgat tagtgtaaat aaatacctat
     9421 tttataatac tcaacataaa gacaccagac atgattagta ttataccaat aaaaatagca
     9481 agccattctt ttttgttctt cttttcttta aaaaatataa tccctcctaa agtggctact
     9541 atcacacaac tctgagaaat agaaaaactt attgtaactc ccaatgtatt agtcgttatg
     9601 aacataccta aatttgctat tgaccacaat ataccagtaa ttaaattgaa taaaacattt
     9661 tccctatata caatctcttt agaaaaatac ttgacaataa tagatgaagt tatcaacata
     9721 ccaacagatt gaggtaatat aatcctatat ccttcaatta aaaatatttg attagttaca
     9781 acatataatg ataaaaatag agatgataat aatacaactc caacacattt taacatattt
     9841 ttatctgtag attttttagt ttgataattt gtgagaagta taccaagtat tatacataat
     9901 attgataaag aaccaatcag taacatttta gaactggtcc attcattaaa caagattaca
     9961 gctaataatg tcgtaaaagt taattgtgaa ccatttgaaa ttggcattac tttagataca
    10021 ctagcaattt gaattgcttt aaactgcatt aattgtccca aactccagaa tatgccagaa
    10081 atcatactta tacaaaatga aaaaaatgta atttcaggat gtataaccag agttattata
    10141 gtggaaaata taaacgcact tatggatgta cctaaaagct gttcatgagg agaacttttc
    10201 tttagatttg caactatggg catcaatccc catcccatta caggaagtaa agcaataatt
    10261 attgtcagca ttaaactatt tcctttcata ttaaatttag acagctttta atttatcatc
    10321 taaaattaaa gtgttcaata cataaaaatt accgtattta atcaggaaat attagtagtt
    10381 ttttagaaaa acattttcca ttaaaataaa gacaaaggga aggagtaaaa ttgaaaatgt
    10441 taactgatat gattcaaaat cgtgaaatta aaataataaa gtttttatta aaagaaggtc
    10501 atacaactgt aaaagcaata gcagatgatt tagatgtttc agaaaaaaca gtttcaaaat
    10561 ctcttaaaga agtagaagca gtgcttaaaa agataggtct ttctttaata agaaaaccta
    10621 agataggtgt ttatattgat ggagatttaa aaaaaatact tccttaccta gataataaag
    10681 gaagtcaaat tcctacaacc agagaggaga gagttatcta tatatattca gtacttttaa
    10741 agagtaatga ttatattact atacaacaac tttctgatga actatatata agtcgtggaa
    10801 ctattgaaag ggatttagca gaagttgaaa aactgctaaa aaacgaaggg atttccatat
    10861 ataagaaacc tagtaaggga ataaagttaa atataagtga aagagataaa agacaactta
    10921 ctgctaaatt tatacataaa ttttggagta gaaactggta tattaagcaa gagcaagaaa
    10981 gcttttatca agcatttgaa aagatacagg cagatgtaaa tggtattttt tcggaagatg
    11041 atttatcaat aattattgat attcttagga atttcagcag agaaagaaaa tttgagttct
    11101 ctgattatga atttcagtca ttagttattc atttggcaat agctgtagag agaattagaa
    11161 aaggcgaata tatagaagaa agtttgtata ttggtaatat agaaaataat caaatgtcaa
    11221 ataccaatta tttagcaaaa caattagaag aaaaattttc aatattatta ccaaaatctg
    11281 aaattggata tataaattta catttagtag cggctgacca acatagagat actaatgaac
    11341 taaaaaatac ttcaactaac ccttgtgaaa caagtacaaa taatatatca aatcttcggg
    11401 aaattataaa aaatacactg tatgagacag cttatgacaa ggatttaatt gatggattaa
    11461 cttcacatgt acattcatca ataaatagac tgaaatatgg acttagtatt aaaaatccat
    11521 atgtaaatac aatcaaacaa aacttttctt tatcatttga gcaagcttta gatttaaaga
    11581 aagtaattga atttatatat gatattagga taaatgatga tgaatgtgct tatatagcat
    11641 tacattttga agcttttaga gaaagaatga aagaacttcc acaaaatatt agagtaattc
    11701 tagtatgcag tacaggatta ggttcatcta ggttattgtc tgcaagaata aaaaaatact
    11761 ttccaatgat aaagattgaa aaagtacttt ccgtacaaga attagtatcc agtaaaattg
    11821 atgcagattt agtaataagt actatttatt tagagttaga aacaattcca accatagtag
    11881 ttagtcctat tttaaatgaa agtgatataa gaaccttgga aagaaatata caaaatgttt
    11941 catgttgtac ttctaagaag tataaatctt tcagaaactt aatctttgaa gaaaatatat
    12001 tgttgaatat tgaagcttca aattatgaag atgcaattaa tcatataaca gataacctaa
    12061 taaaaaaagg ttttgcaaaa gaaggtatag ctaaaagtgc aattgagaga gaaaatttat
    12121 cttacacttc tttccaatca atagctacac ctcatgctaa tcctagttat attaaaaaat
    12181 ctaatatatc tgttgcaaag ttaaaaaatc ctattacatg gggaaatgaa aaggtggaag
    12241 ttattttctt tatttcatta gaaaatgaca aaaacctaga gcttgatgaa atatatgaag
    12301 ttttttatga ctttattgac aataaaaaaa atataaatga catattaagt gcaacttcca
    12361 caaaggaagt gtacgatttg ctagtggagg agagtatata gtgaaaatat cagatgtatt
    12421 gaaaaaagag caaattgttt ttaatcttga tacaaataca aaggaagaaa caataaaaaa
    12481 attaagtagt atatttgttg aaaatgggat tgtggacaat gaagaagaat atgttaagtc
    12541 tgttttggaa agagaagaac attcaactac aggtattggt aatggtattg ctattcctca
    12601 tggaaaaagt aatgctgtca aaaaatcttc tataatattt gcaaagacta agcataagat
    12661 tgagtgggat gctttagatg aaaaaccagt agatagtatt tttttactgg ctataagcaa
    12721 tattgataag gatagcaatc atcttgtact attatctaaa ttagcaacta aacttatgga
    12781 tgatgacaat gtagatgcac taaaattagc caattcagaa caagaaataa tcaatatatt
    12841 tagtgaagaa ggagagatgt agaaatgaaa agaaaaatta tagcagttac agcatgtgca
    12901 acaggagttg cacatacata tatggcagca caagcattaa agaaagcttc aaaaaagatg
    12961 ggtcacctta taaaagttga aacacaagga gctacaggaa tcgataatga gttaactaca
    13021 aaggatgcac aaattgcaga agtagtaata tttgcagtag acactaaagt cagaaatgct
    13081 gaacgttttg agggaaaaga aatactagaa gtaccagtta atgcacctat aaaggatgca
    13141 gagaaagtaa taaatgatgc attaaagtta attgacacta aataaatgga gggaaatgta
    13201 atgttgaaaa agatagtctc tgagttaaaa aagcatgtct taactggaat ctcatatatg
    13261 attccattag taattgcagg tgctatgata atggcgatat ctcgtgtagg tggttctttc
    13321 tataatatac cagatatatg ggatgctaaa tatgcagaaa gtgctagtag tattgttaga
    13381 ttattacatg atttagatgg gtttggaggt actgcattag gattaatgtt tcctgtaatt
    13441 gctgcattta taggattttc tatagctgat aaattagcta ttgtaccagg tcttgttgga
    13501 ggtatgattg ctaaagatat aggtgcagga tttttaggag ctttggcagt tggtcttata
    13561 gcaggatata catgtttgtt tattaaaaat catgttaaat taccaaaatc agcagcttca
    13621 atagtaccaa tatttatagt accagtattt gggacactaa tcactgttgt attaatcaat
    13681 tatgtgatag gtataccttt tgccacatta aatactggat tagaaaattg gttaaatgga
    13741 ttatctggta caaatcagat actaatggca gctattatag gggctatgat ggcagttgac
    13801 ctaggtggac cagttaataa agcagcttta acaacatcac ttgcattatt aacaagtgga
    13861 atttatgcgc ctaacactgc tgcaatggta ggaatagtta taccaccact aggattggga
    13921 cttgctacta tactagcaaa acaaaaatat aataaacagt tacgtgaagc tgggaaatca
    13981 tctcttataa tgggtttaat tggagtaagt gaaggagcaa ttccttttgc ggtagaatct
    14041 ccattaaaag taataccttc atgtgtaatt ggaacagcaa ttgcttcagc tatggcagtt
    14101 ggtttaggtt cagtaaatgc aactccaatt agtggatttt atggatggtt tacagtagag
    14161 aattggccaa tgtatgtgtt atcaatagca gttggaacag taattgtagc tggattagtt
    14221 atagtattgc gtggaaatcc agtgattgag gatgatgaat ttgaagatga tgattttgat
    14281 gaagcagagt gggaatctta aaatttaata actatttaag acgtgttata agctatattc
    14341 aatatgaatc ttatactaaa taaagtgtta aatattcagt ttagacttaa atataaaaga
    14401 taaagaacag aggagaataa atgataaaag cacatattgt taatcataca cactgggata
    14461 gagaatggta ttttacttct ggagatgcat tagtacttag tgaacaacta tttacagatg
    14521 taattgatga attagaacgt aatccagatg taagttttgt acttgatgga caactttcta
    14581 ttttggatga ttatgtacaa ttacacaaag aaaaaataga aatcattaaa aagttaataa
    14641 aagaagatag actacatatt ggaccatggt ttactcaaac tgatgcattc tttgctagag
    14701 gagagtctat tttaagaaac ttaatgattg gaatttttga aagtaaaaag tatggtaagt
    14761 atatgaaaat tgggtatttg ccagatacat ttggatttaa tgcacagatg cctatactat
    14821 taaagcatgt aggtcttgat aatatattct tttggagagg tatacatctt ggtaaacagg
    14881 ttcaatcacc atattttaag tggaaaagct taaatggaga aagttatata tatgcagtaa
    14941 atatgccaca tggatatgga actggtatgc ttttagagcc ttcattaaag tatgttaatg
    15001 gacgcttaga cccagctatt gattttatta aatcatatac tgatgtagat gaggtgttaa
    15061 taccttctgg gaatgaccag ttaaatataa taggtgattt caaaaataaa attaaagcga
    15121 ttaatgatat cggaaagtat tcatatataa cgagttcata tcaagagttt ttaaaatata
    15181 taaagtccat agaatcacta gaaagctata gaggtgaatt tagagaacct gtattagcaa
    15241 gaatacataa atcaattggt tctagtagaa tggatataaa attagcatgt gataaactag
    15301 aaaacaagct gattaaaaga atagagccat tattagtaat tgctaaaaag agtaaaattg
    15361 aaataagcaa tcagttactt ataaatacat ggaaaaaact tttggaagga caagcacacg
    15421 atagtttagc tggatgtgta actgatactg taacagatga tattcttcac cgtataagag
    15481 aagcaaatga aatatgtgac agtattgaaa atactattgt aaaaaagatt tcagaagggc
    15541 ttaaattatc taaaaatgat attttagtat ttaatacaga agctaagcgt tttaatggat
    15601 ataaagagat acaagttgta agtgatagta aaaatattta ttttaaagat aatttagatg
    15661 ctactataat tgaagaaact tatgtaaaac cgagagaaaa tgtattagaa gaaacacctg
    15721 ctggtaatat atttattgaa gaaccaggat attatatatt aaaagtaaga ataaatatac
    15781 agcttcctgc acttggttat aaagttgtta gttttgaatc aagtgataaa gaaatggtat
    15841 cacttgataa atctaatgac acttttatat ctaatcataa ttataagata atctatgaag
    15901 atggaagctt aaatttacaa cttaaagatg gaacttacat atcaaacttt ttaatattaa
    15961 aagatagtgg taatgcaggc gatacttatg acttttctcc attaaaaaat gacgaggata
    16021 taaaactttc atttgataga gtgtatacag aaaaagcttt aggatatgaa aagttggtgg
    16081 ttaaaggtac tactttacta ccacttacat tagaagatag aaaaaacaaa tatcttaatg
    16141 ggaaattaaa tgtaaaagta agtattgtct tatccaaaga aaatccattg atggatgtaa
    16201 aactttgtgt tgataacacg atatataatc atagactacg tgttcatata aaaactgata
    16261 taaaagataa taaaaacata gcatcattgc catttggata tataacaaga gaaaatggag
    16321 ttttagacaa ctgggaagat atttacagtg aaatgcctat agatttagaa cctttggaaa
    16381 gtaatattac tctaacaaac caaaatagaa gctgtactat atttactaga ggaataaaag
    16441 agtatcaaca tattgaagat gaaattgcat tgaccttact tgctactaca gatgaactag
    16501 gaaagcctga tttattatat agaccaggac gtgcttctgg tgatacaact aaaaaaggtc
    16561 acataagaat taaaacagat aaagctcaaa tgcttaagaa attagaattt tcatttgcaa
    16621 tatatatgga ttctaaatca tttgatgaat tggaaattgc gaatttaacc cacaattatc
    16681 taagggaatg tgtaaattat caattacagg attacaattt ctttttatat cgtatagata
    16741 ataagattca aaaatcaatt gtcaataaaa ctgtaagtag agaattagaa ataatatctt
    16801 taccagaaaa ctatcttata tcagcatgtt atccttctta ttacagtaaa gataaattta
    16861 taataagagt ggaaaatcca agtagtaata gaatgatatt ggatgagcaa atatttaaaa
    16921 atagaaatgg gaaaattgta aatgcaatag aagatgtaga agaagaacag gtatatgaaa
    16981 tagcaccttt tgacgttatt tcaatattat tggatttata agtgtaaaag taagtttatt
    17041 gtttaaagtc tgacagatat taatctgcca gactttttaa cttgtataaa tttgggtaaa
    17101 tattaatgaa tggttttagt atttaagtat gaatttagta tttaatatag aatttattga
    17161 agtagaataa ttagtttaac aggataagca gtttaaataa agaattatta tattagagtg
    17221 attttattat ataatttaaa ataataaaat tttaataaaa tgagttaaag attgtatatt
    17281 aatagagttg atgtagttac aaaaaagtta ccaattgtta tgtatttaca aaactgatat
    17341 attaattaca taaaatctaa aatagtttgt aataggagta tatataataa aaattttaat
    17401 taaataaagc taggtatatt tataatatta ataaatatat tgctataaaa gtttgtttaa
    17461 taaattaaat aaagaaaaga ggagaataaa atgtacaaca tagatgatat gatatataat
    17521 tgtccagtag aagctttatc aaatatttta ggtaaaaaat gggtggctat aattatatgg
    17581 aaaatacaag aagataaaaa acgatttgga gagctacaaa gagctatacc agactgtagt
    17641 aagaaaatgt tagttcaaca actagatttt ttaattgaac aggggattat agtcaaccaa
    17701 aaaaagttgg tcaataatgt tgtagagtca acatattttt taagtgaatc aggattacga
    17761 ttgcttcctg tcatggaaaa aatgattatg tggagcaata aaaatttaaa ttgtgataaa
    17821 tactaaaatt tactctgtaa gtaatgaaaa tttaattaag ctatgagtag aaaaatgtat
    17881 taaaattaca taatttttta gttagatttt aatttagaaa tgggattgta atattggaaa
    17941 taaaataagg aggaatttta atttgcaaaa tgtaaattta tttttagtat ttatagaagg
    18001 tatagtatca ttcttttcac catgtatact gccaattttg cctatttatt tgagtatatt
    18061 gtcaaatagt agtgtagaaa atttaaaaga aggaaagacg agttttatag gaagttcttt
    18121 atttaaaaat actatatttt ttgctcttgg aatctcaaca acattcttta tattgggttc
    18181 atcagtaaaa gtattgagta tgttttttaa tgaaaataaa gatttaataa tgtttattgg
    18241 tgggattata attattatca tgggattatt ttacatggga ataattaaat cttcaatatt
    18301 gaatagagaa aaaagattta atgttaagtt taaagaaatg aaagcaataa cagcttttat
    18361 attaggtttt acgtttagtt ttggatggac accttgtatt ggtccaatat tggcatctgt
    18421 attagtcatg gtttcaagtt caagtaatca tcttagtgcc aatcttttaa tagcagtata
    18481 tactattggt tttattttgc cattcataat tacagctatg ttttacagta aactatttaa
    18541 aactatagat aagataaaat ctaacatgga aattattaaa aaaataggtg ggattatact
    18601 tatagtatca ggaatattaa tgatggtaaa tggatttgga agtataagca aacactttaa
    18661 tacgtcacaa aatagtaaaa ttgaaagcaa gcaagaagaa aataagcgag aaaattctac
    18721 tgataaagaa gaaaattctg atggtaatga tagtcaaaaa gactctaata atgacaataa
    18781 tgataaaggg tctaatgatg aagatagaat aaagtctatt gattttactt taacagacca
    18841 gtatggtaaa acacataaat taagtgatta tgaaggcaaa gttgtattct taaatttctg
    18901 ggctacatgg tgtcctccat gtaaagaaga aatgccttat atagaacaac tgtataaaga
    18961 ctataataaa aataatgatg atgtagtaat tttaggagta gcttcaccta atttaggtag
    19021 agaaggttca agggaacatg tagttaactt cttaaaagac caaggttata cattccctgt
    19081 tgtacttgat gaagatggag cattagcata tcaatatgga attaatgctt ttccaactac
    19141 atttataatt gacaaggaag gatatgtaac tcagtatatt ccaggagcta tggataaggc
    19201 aactatggca tcatttattg aaaatcagag aaataaataa aagtatttaa gtataagttt
    19261 ataaatcaat ttaaggttac aaaaaagtgc ctaattgtac cacctaaata atcttgttat
    19321 agtaaaagta taaacaatac aagaaagggt tgaattttat atgaaaaaaa catttgatgc
    19381 tataataata ggatttggaa aaggtggaaa aacattatca ggggatttgg caaacagggg
    19441 attaaaagta gctttaatag aaaagtcaaa caagatgtat ggtggaacat gtgtaaatgt
    19501 ggcatgtata ccaacaaaaa gcttagaaaa ttcagcaaac agcgtaaaaa caaagaatat
    19561 aaattcttgg gatgaagtac aagctgaata tgagaaagct atagataaaa aagaaacttt
    19621 aatcacaaaa ctcagagaag ctaactataa taaattaaat tcaaatgaga atgtaactat
    19681 attcacagga atgggaactt tcattgatga aaaaacagtt caagtcaaaa ctgaaactga
    19741 aatttatgag ttagtagcag ataatatatt tattaatact ggttcaagac catttatacc
    19801 taatattaag ggaattgaaa ataagaatat agtatatgat agtgagtctc ttatgaatct
    19861 aagaacttta cctaaaaaaa tgacaattat aggagctgga tttataggtt tagaatttgc
    19921 tggtatttat agttcatttg gagcagaagt tacaatatta aactctaata atggcatttt
    19981 accaaatgaa gatgttgagg attcagaaga aataataaaa ttacttgcaa agagaaatgt
    20041 aaaaattgtt aataatgcaa atataaaaga gattaaagaa gtttctgaat tagcaatagt
    20101 agaatatgaa gtagatggta aatctaaaga attaacaagt aatatgatac ttgtagctac
    20161 aggaagaaaa gcaaacactg aagggctagg gctagaaaat gcaggtatag aattaaatga
    20221 aagaggattt attaaagtct cagaaactct taagactaat aaagagcata tatgggctat
    20281 aggagatata aatggaggtc cacaatttac ttatatttca ttggatgatt atcgcatagt
    20341 tattaatcag ttgtttggag ataagactag aacaactaat gatagaaaga atatcccaaa
    20401 ttctatattt ataagtccag cattttcaag agttggtctt aatgttaagc aaactaaaga
    20461 aaagggatat gaagtattag tggcaaagat gccagtagaa gcaattccaa gagctaaaca
    20521 gattggcaag gcagatgggt ttataaaaat agtgatagat aaaaaatcca ataagatact
    20581 tggagctagt atgatatgtg aaaattcaag tgagattatc catcttattc aattagctgt
    20641 agatttagaa gtagagtata catatttaag agaccgtgtt tatgcacatc caactatgac
    20701 agaagctctt aatgatattc tatctcctaa tatgataaaa gaagtataac ataagtttaa
    20761 tatataaatc taaaattatc attataagta gattttagaa agaggctatc tcaaaaaata
    20821 gagataagtc tcttttttac tgtttaaata ataaaaatat atttgatttt ataaaagaag
    20881 attattttat gagctaaaaa gtttatttag aaacactacc tatttatgtt ataaagttat
    20941 aaatataata tagaatctat tattctgtat tattaagtgg ggttaagcgt tataattaat
    21001 tataaatatt gaataatcta aatggataag ataatggtat taaaaattat cttaaaaggt
    21061 gaaataatct atatttaatg tagtacagtt atatctttaa agcaaggtgg tgaaatgata
    21121 taaataagat atctaaaaga gggtttgatg atagtgataa aagatggttt tcaagtaaag
    21181 aattaacaag acttataaag gctaaagaag aaatagaatg gttattaaat agggattaca
    21241 aaatagaccc agtcgttact tttgttggtg atagatatca gttttctata agacaaagag
    21301 atgcattaaa aagggcagtc tgtactgatg aaaaaaatat tataagacaa tcaaaaaggc
    21361 tttctctaga taaaatcaaa gaagataccg taaatataga tggctttaat ttgataatat
    21421 ccatagaggt agcattatct ggtggtactc tagtaatagg aagtgatggc aatataagag
    21481 atttagcagg tcttagggga acctataaga ttatagataa aacagaagaa gctttgaatc
    21541 tgataggtaa attttttaat aaatacaatg cacagaatct taaattttat ttagatgctc
    21601 cagtttctaa ttcaggaaat ttaaagtata ggatattaga gcattccaaa acttggggaa
    21661 ttgaaactga agtagaatta gtaaaaaatg cagatgttgt attggaaaaa ttagatagag
    21721 tagttagtag tgatgcagtc atagttgaca agtgtataag ttattttaat gtggctagag
    21781 gtataattga agagtatatt aaagaatgta atatcataaa tttaaataaa tagataaata
    21841 taggtgagaa tatataagta tatgtagcaa tattattagt aatacaagta aagtttgaaa
    21901 ataggagaac gaatacatat cgaatttcat aaaatgttgt aaaaagacat atgagaggat
    21961 ggatatgtgt gaaattaact aaactaaata ttaaaaaata tagagcacct atatataaat
    22021 atgctttagc aaatgtaaat ttgaggtctg ctaaatctac caattcaagt atcataacag
    22081 taatacctca gggagccaaa atggaagtat tggatgaaga agatgactgg attaaggtga
    22141 tgtacaattc tcaagaagga tatgtgtata aggatttggt atcagtaagc gaatatgctt
    22201 ggagtaactt aaacttaaga gaagataaat ctacaacttc caatataatt acagtaattc
    22261 ctgaaaaatc tagggttgaa gtccttcaag tagatggaga ttggagtaag gttgtatatg
    22321 atgataaaat aggatatgtt tttaattatt ttttatcaat tgatgggaat aaaccaaatg
    22381 aattagatta taagtatttt tatactgaca tgattaaatt tgttaacgaa aataatatta
    22441 aaagtactag cgattatcta attgtaacag accttagaaa taaatatact tatattttta
    22501 agaaagataa tggaggctgg ggacaacttt acaagtggca atgtactata ggtaagcctg
    22561 agactccaac tataacaggt atattttata taagtggaag aaaaccttct tttggaacgg
    22621 atgagtattc agttaagtat gctactagaa ttaaaggtgg atattactat cattctgtac
    22681 tatatgattc aacaggaagt tatataatag atggcagact tggtgaagct cttagtcatg
    22741 gatgtataag attaagtacg gaaaatgcaa agtggatata tgataatata ccagatacaa
    22801 caacagttat aattcattaa gaaaaatttt gagtttttta ttgggataat gattcttaaa
    22861 ttaataggat tatatattaa cttctcataa agagtactat ttaaaaagtg taataagaga
    22921 gtgctattaa caaaacaaag atattttgag gcactctctt ataaatatac ataattaaga
    22981 tattttcata aacaattcta taatcttatc tttatcaaag aaaacaggat tagcaccagc
    23041 acaagcatct ttcatagagt tttctgcaag caattcaaaa ttaggattat caacacctac
    23101 atcttttaaa gattttggga ttccaacctc ttcagaaaga gcttttatct tatctattac
    23161 aaagtcaaca cactctttat ctgatttatt atttacatcc attccaataa cttcagctat
    23221 tggtcggaat ttggcagggg ctgatttagc attttcctcc tcaactattg gaagtagcat
    23281 ggcattacat actccatgag gtaaatcata taaacctcct aattgatgag ccatagcatg
    23341 tacatttcca agacctgcat tactaaaggc aattccattt aaaaaacaag cataacacat
    23401 ttgttctcta gcttctatgt cattaccatt tttaacagct cttggaaggt atttaaatat
    23461 ctgctttata gcatataagg cagttgaatc tgtaacatca taagcaccat tagctactac
    23521 agcttctata gcatgagtta gtgcatccat accagttgct gctgttaaat tagatggttt
    23581 gctaatcatt aattctgggt catttactgt cattgtggct agtgaattgg tatcaaccat
    23641 tatcatcttt acatgtctgt cttcatcagt tataacatag tttattgtaa cttcagcaga
    23701 tgtaccagca gtagtagtta tagctatgat aggaagacat tttttagaag ttttgttgat
    23761 accttcatag tctctaatat ctcctccatt agtggctaat atagagatag ctttaccaca
    23821 gtcttgtgga gaaccacctc ctatagttat aacgaagtcg caattttctg attttaatat
    23881 tttaagtcct ttattcacat tagttacagt tggattttgt ttaacttcat cataaacaac
    23941 aaagtctata tctatctgag ataaaatatc tgttactttt tttacagtac catttgaaac
    24001 tagaaattta tcactaacaa taaatgcttt tttagttcct aatgatttaa ttgaatcttt
    24061 agcttcatta agacatcctt tcccaagtaa atttattggt ggtacataat aaatgctcat
    24121 aactaaaatc tccttttatt ttcattgatt tttatgtaaa aatatatatc taacaatttt
    24181 cactaattta ctcataatat atcgtatctt agattgagat agggttgctg gttcttatat
    24241 aagaacttaa ttcgtaatta cgattatgat tagaatttaa catagtattt ttatcatgtc
    24301 aataaaaata taaaaaatat tataaaaaaa acaaagttac tgtagttaaa tcagcaactt
    24361 t
//
LOCUS       DAEMHW010000044        23951 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEMHW010000044 DAEMHW010000000
VERSION     DAEMHW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.044"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..574
                     /locus_tag="KPK67_001504"
     CDS             <1..574
                     /locus_tag="KPK67_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2458494.1"
                     /translation="LFEKTKKEIKYYKILFTVILIAILGSTFLYNRLKYFKAQNLHDG
                     LTNVYNRKSFDIIYEKYREKDDNFALVMIDIDNFKLINDNYGHKFGDTVIKGITNTIC
                     VMLEQDDKVFRYGGEEFSVLIRNKSGEEVADIIDSIRVAIANKKWNDDVTVTISAGVA
                     HVSDSKDVLEEADKNLYKAKQLGRNKVVYK"
     gene            605..2399
                     /locus_tag="KPK67_001505"
                     /pseudo
     CDS             605..2399
                     /locus_tag="KPK67_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            2750..2995
                     /locus_tag="KPK67_001506"
     CDS             2750..2995
                     /locus_tag="KPK67_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG2458495.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            3030..3756
                     /locus_tag="KPK67_001507"
                     /pseudo
     CDS             3030..3756
                     /locus_tag="KPK67_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
     gene            3778..5091
                     /locus_tag="KPK67_001508"
     CDS             3778..5091
                     /locus_tag="KPK67_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2458496.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYGENLKKIV"
     gene            5297..5836
                     /locus_tag="KPK67_001509"
     CDS             5297..5836
                     /locus_tag="KPK67_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2458497.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            5925..7910
                     /locus_tag="KPK67_001510"
     CDS             5925..7910
                     /locus_tag="KPK67_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBG2458498.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            8134..9102
                     /gene="spoIIIAA"
                     /locus_tag="KPK67_001511"
     CDS             8134..9102
                     /gene="spoIIIAA"
                     /locus_tag="KPK67_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBG2458499.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            9092..9613
                     /locus_tag="KPK67_001512"
     CDS             9092..9613
                     /locus_tag="KPK67_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBG2458500.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            9630..9827
                     /gene="spoIIIAC"
                     /locus_tag="KPK67_001513"
     CDS             9630..9827
                     /gene="spoIIIAC"
                     /locus_tag="KPK67_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBG2458501.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            9843..10229
                     /gene="spoIIIAD"
                     /locus_tag="KPK67_001514"
     CDS             9843..10229
                     /gene="spoIIIAD"
                     /locus_tag="KPK67_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBG2458502.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            10314..11519
                     /gene="spoIIIAE"
                     /locus_tag="KPK67_001515"
     CDS             10314..11519
                     /gene="spoIIIAE"
                     /locus_tag="KPK67_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBG2458503.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            11579..12196
                     /locus_tag="KPK67_001516"
     CDS             11579..12196
                     /locus_tag="KPK67_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBG2458504.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            12208..12762
                     /locus_tag="KPK67_001517"
     CDS             12208..12762
                     /locus_tag="KPK67_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBG2458505.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            12789..13478
                     /locus_tag="KPK67_001518"
     CDS             12789..13478
                     /locus_tag="KPK67_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBG2458506.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNIDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            13588..13950
                     /locus_tag="KPK67_001519"
     CDS             13588..13950
                     /locus_tag="KPK67_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG2458507.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            14035..14541
                     /gene="nusB"
                     /locus_tag="KPK67_001520"
     CDS             14035..14541
                     /gene="nusB"
                     /locus_tag="KPK67_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBG2458508.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            14525..15595
                     /locus_tag="KPK67_001521"
     CDS             14525..15595
                     /locus_tag="KPK67_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBG2458509.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            15592..16797
                     /locus_tag="KPK67_001522"
     CDS             15592..16797
                     /locus_tag="KPK67_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBG2458510.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            16799..17014
                     /gene="xseB"
                     /locus_tag="KPK67_001523"
     CDS             16799..17014
                     /gene="xseB"
                     /locus_tag="KPK67_001523"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBG2458511.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            17017..17904
                     /locus_tag="KPK67_001524"
     CDS             17017..17904
                     /locus_tag="KPK67_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBG2458512.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            17934..18371
                     /locus_tag="KPK67_001525"
     CDS             17934..18371
                     /locus_tag="KPK67_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBG2458513.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            18405..20270
                     /locus_tag="KPK67_001526"
     CDS             18405..20270
                     /locus_tag="KPK67_001526"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBG2458514.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANNNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            20403..21215
                     /locus_tag="KPK67_001527"
     CDS             20403..21215
                     /locus_tag="KPK67_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBG2458515.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDSSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            21235..22914
                     /gene="recN"
                     /locus_tag="KPK67_001528"
     CDS             21235..22914
                     /gene="recN"
                     /locus_tag="KPK67_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBG2458516.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGSFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            23128..>23951
                     /locus_tag="KPK67_001529"
     CDS             23128..>23951
                     /locus_tag="KPK67_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458517.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKFTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKD"
BASE COUNT         9779 a         2331 c         4452 g         7389 t
ORIGIN      
        1 gctatttgaa aaaactaaaa aagaaataaa atattataaa atattattta cagtaatttt
       61 aattgctata ttagggtcta cttttttata caaccgattg aagtatttta aagcacaaaa
      121 tttacatgat ggtcttacaa atgtttataa tagaaaaagt tttgatatta tttatgaaaa
      181 gtacagagag aaagatgata attttgcact tgtgatgata gatattgata atttcaagct
      241 cataaatgat aattatggac ataaatttgg tgacactgtt ataaagggaa taacaaatac
      301 catttgtgtt atgttagaac aagatgataa agtctttagg tacggaggag aagaattttc
      361 tgtattaata agaaataaat ctggtgagga agtagcagat ataattgaca gtataagggt
      421 tgcaattgca aataaaaagt ggaatgatga tgtgactgtg actataagtg caggtgtagc
      481 tcatgtttca gatagcaaag atgtgctaga agaagctgat aaaaatttat ataaagcaaa
      541 acaacttggt agaaataaag tagtatataa gtaattatat actatataaa taagaggtat
      601 aaatatgaaa caatcaatag ctatagtaac tgataaaata actaaactat catctttctt
      661 agaagaaaat atacgtttgg ttttaggtaa ttatattgat ataaaccact attatctgga
      721 agttttaaaa gagaatgata aaatacaagg tgattttgta ctagtaatga atgatgatag
      781 attaaacagt atgagaaagc atttagtaca agatagtaag attatagtga taaggagaac
      841 acttaaagaa aatgaaatat atggattatt ttcaatacca aatggaactg aagtattggt
      901 tgtaaatgat acaaaagaaa ctaccttaga aacgataagc ttattttaca aaataggagt
      961 aaaaaattta aagtttactc catatattga aggaaaaatt tataaaaata tagacatagc
     1021 tataacacct ggagtaaagg gaaaagtacc aagttatata aataaagtaa tcgaccttgg
     1081 aaatagatat attgatatat ctacctttat agaaataata aatggtctta gaatagatgt
     1141 taaagaaata cgtaaaaatt taatggcata ttctgagagt ttaataagtt tagataatgg
     1201 agttaaagat aattataggc aactgttttt aaaaatagaa gaacaagatg ttatattgaa
     1261 tctgtcaaaa gatggaataa tatttacttc agcagatggt atattataac ttacaagaaa
     1321 taacgtatat aaaaaaatta gaacaaaatc ttactaataa attaagagaa caaggtcaga
     1381 tagcaagata tacatttaaa gatataaaaa ctaaaaataa aaatatggta aagtgtgtag
     1441 aattggctaa aaaaatatct aaatcagacc tttctgtatt aataatagga gaaagtggta
     1501 ctggtaaaga attattatca caatcaattc ataatgcttc caatagaagc aatcaaccgt
     1561 ttatagcagt taattgtgca gcagttccag atagtctatt agaaagtcaa ctgtttggat
     1621 atgaaaaagg ctcttttaca ggagctttaa gagaaggaaa aaagggtcta tttgaacttg
     1681 ctaataatgg aactatattt ctggatgaaa ttggagatat gcctccattg ctacagacaa
     1741 agttactaag ggtattacaa gaaaagcagg ttatgcctat aggttcccat aatgttataa
     1801 acatagatgt tagggtcata gcagcaacaa acaaaaactt actaaagatg attagagaag
     1861 gaaaatttag agaggacctg tattatagac taaatgtatt gccaatagat gtaccatcat
     1921 tgaggaatag aaaagaagat ataataacac ttatgaattt ctttttgaaa aacaatataa
     1981 gactttcgcc agaagttata actattttag aaaaatataa ttggccagga aatataagag
     2041 agttacaaaa tgttgcttct tatgtaagtc taatgtgtga tagtatggtt tttagagatg
     2101 acttaccacc atatataagg gtagaatgtg ctaacaatta tgaagatgga tttggttata
     2161 caagaaaaga atttgaggaa atactgagga ttctatatga aagaagagag tcatcctctg
     2221 gaattggaag agggtttata atcgaaaagc ttatacagga aggtattgag atttctgaaa
     2281 gcaaagttag aaaggtgcta tcttatttaa gtgaatgtgg atacatatca tctaaatctg
     2341 gtagaggagg aagtcaaata acctcagagg ggataaaact ttataataat ataaattaag
     2401 ctaagtattt gatattgaaa tcacatattt tgaatttaga tatttaaata ataataaact
     2461 tttatattta tagagattaa tttatattat gtcagaaaga atattgtaat ttataattaa
     2521 atattaatag gtttttaaaa taggattaga taggtaatag gttaagttag aataacccat
     2581 tacctatttt tatttgtaag aaaagtgtac atagaattag aagtaaaaaa tacaactaat
     2641 aaaactattg aaaatactag ataaataata aataaaaata attatagatt ttggcataca
     2701 tattgctaat tatagagata agacgacaaa ataattggga ggtttaatga tgaaatacaa
     2761 aacaacaggg acttgtgcta cagaaattga atttgaagta aaagaaaata aagttacaaa
     2821 tgtaaacttt ataggtggat gtgatggaaa tttaaaaggg ctaaaagtat tagttgaagg
     2881 tatgaatata gaagatgtaa tacaaaagtt aaaaggaata gagtgtaaaa caaagccaac
     2941 atcttgccca gaccaactgt ctctagcatt ggaaaattat atgaatataa agtaatacat
     3001 aaaacaatcg attagttagg agtttaatta tgaaaccaat aatagggatt ttaggaaatt
     3061 taataataat ggaaaatggt atgtttccag gtcttgaaag gtcatatgta aataatgatt
     3121 atataaatgc agtgctaaaa ggtggcggta gtccagtaat aattcctgta aatacagata
     3181 aagaagttat aaaaaaacag attgaaatgg ttgatggagt cttaatatct gggggatggg
     3241 atatcaatcc tcaactgtat ggagaagaaa caagagaaga aacgacattt atatatccag
     3301 aagtggatga atttgattta attgcaataa gtatagcatt agaacttaaa aagcctatat
     3361 taggtgtatg tagggggact acaaatttta aatgtaagtt taggaggaac tttatatcaa
     3421 gataataatc ttatagaagg aagttatata aaacatacac aatcttctaa aagacatgta
     3481 gctactcata aagttgatgt taaagaagga agtattttag agggaatttt aggaaaacaa
     3541 ttacttacaa atagttatca tcatcaaagt gtcaatcagc ttggaaaagg attaaaagcc
     3601 atagcatact caaaagatgg tataatagaa gctattgaga aagaggatga aaattttgta
     3661 gtaggagtac agtggcatcc agaaatgatg gtagattact gtgataaaat ggaaaaacta
     3721 tttaaatatt ttataaatat ttgttcaaat ttataatata ccttgggggg attgaacatg
     3781 aacgaaaaga acaaaatggg attaataagt attatacttt taggtattaa tgcagtagta
     3841 ggagctggag tttttctatt accaggagat gcaatgaaat catttggtgt agcaagtata
     3901 tttgtatata tatttgatat gttgttagtt ttatctatgg cattctgttt tgctgaggtt
     3961 gcaggtaaat tcaataaaaa tggtgctgcc tatgtatata caaaagaagc ttttggagat
     4021 ttttgtggtt ttgaagtagg tcttatgaaa tgggtaatag gatgtatttc ttggggagct
     4081 ttaatagtag gttttcctac atcattatca gcagtatggg ctccagcagg tgaaccacat
     4141 atacaaaaaa ttataattgt agccatgata gttgggctta caattataaa cttattaggt
     4201 gtatctctat caaaaattgt acaaaatgtt ataactgtag gtaaactaat tccattaata
     4261 ctatttatag gaataggtat attttttata aaaggagtta attttacttc ttcaacaatg
     4321 gtacctccag gagctggagc aacagagttt ggagcagcag cacttttaat gttttattca
     4381 tttacaggat ttgaatctat agcagttgca gcagaagata tggaaaatcc acaaaaaaat
     4441 ataccaatag caattataag tgtaatagta attgcatcaa taatatatat attaaatcaa
     4501 gttgtatgtg ttggtatact aggagattcg ttatctagta catctacacc agtggcagat
     4561 gctgcaagaa tatgctttgg aaatatggga gctggattgg ttacctttgg aacattggtt
     4621 tctgttggtg gaatttgtat gtgtggtgct tttgtaaatc caagaagttg tgtagcttta
     4681 gctgatgata aaatgttacc gagaatattt gcaagaaaag ataaaaaagg tacaccatat
     4741 gtagcgataa tagctactat gttaatcact ataccaatag ctctatctgg aagttttgct
     4801 gaattagcag caataagtgc agttgctaga ttcatacaat acataccaac atctttatcc
     4861 gtattagtgt ttagaaagaa aagaccagaa ttagtaggta cttttaaaac tccttttgga
     4921 gcagtgattc ctctaattgc tgtatgtgtt ggaacatggt tattattcca agcatctatg
     4981 catcaattaa ttatgggatt aggagctttg gcaattggag taccattata ctttataatg
     5041 aagtcttata atagaaaagt ctatggtgaa aatttaaaga aaatagttta attatgttat
     5101 aacagtttat tgagttaaaa ttagacattg gcagaaaatt aaatacaaat aagtatctat
     5161 aaggctatat taaaatatta ttaatttttt attttaatat agccttaaat tgttatcaat
     5221 aagtgaaaaa tattgtaaca atagaagaac aatagtatta tgtttataga caaaaaactt
     5281 attaggaggt aaatttatgc caacaataac attaaaaaat ggtgtagacg tacttattag
     5341 agaaggagta agggaagatg ctcaaagcat tatagatttt tataatgaag tgggtggaga
     5401 aactcatttt ctgtcatttg gaaaggatga atataaaata tctttggagg aacaagaaaa
     5461 tgctatagaa tcagcaaaag catctgataa ttctgttaaa ctgattgcat ttatagatgg
     5521 tgaaatagtt gggattgcaa cgattgattc taatcaaaaa gcaaaaggaa agcatgttgg
     5581 agtattagga attgttgtaa aagaaaaata ttggggaatt ggattgggta aaaggcttat
     5641 gttagacctt atagaatggt gtaaatcaaa tggaataacc aagaagataa ctcttgtcac
     5701 taatgaagaa aattataatg ctataggatt atacaaaaaa gttggatttg aagtagaaag
     5761 catactaaag aaagaatgtt attataatgg tgtttatact gatttaatag gaatgtcact
     5821 tttattggga atataattag tgtacatttt atctcgaaaa aagtatatta agtatggtat
     5881 aattaaaaaa gaatataact taatataatt ttggaggacg aattatgaaa aatctgtgta
     5941 aaatatcaga tgattattta aacagcaagc ttaagtattt aaagttatta tcaaaacaat
     6001 atccaagtat atcaaaagca agtacagaaa taataaattt agaggctata ttaaatcttc
     6061 caaagggaac agagcatttt ataacagatg tacatggtga atatgaacca tttgtacatg
     6121 tattaaaaaa tggttctggt gtaataaaga gaaaaataga agaattgttt tcaaatacaa
     6181 taagagacag cgaaaaaaaa atgttagcaa ctcttgtata ttatcctgaa caaaagttag
     6241 acctaataat taagcaagag gaaaatatag atgattttta tagaataaat atttatagac
     6301 ttatagaact ttgtaaatat gcttctagca aatatactag gtctaaagtt agaaaactgt
     6361 taccagaaaa ttttaaatac ataatagaag aacttttgca tgagcatgtt aaaagtgaac
     6421 ataaagaaga gtattataaa agtatagttg aaacaatagt tgacatagga atagctaagg
     6481 agtttataat tgctatttct acagttatac aaaaattggt tgtagataga cttcatgtaa
     6541 taggagatat ttatgataga ggtccaagac cagatataat tgtcgataag cttattgaac
     6601 atcattgtgt agatattcaa tggggaaatc atgatatatt gtggatgggt gcagcctcag
     6661 gtgaaaaaac ttgtatagca aatgcactga ggatatcagc tagatatgcg aatttagaca
     6721 ttgtagaaga catatatgga ataaatctat tgcctttagc cacttttgct atagaaatgt
     6781 ataaagatga cccttgtaaa gaatttattc caaaggttaa tgaccaaagt gtaacaacaa
     6841 cagaaaagtc tttaatggca aaaatgcaca aagcaataag tataattcaa tttaagcttg
     6901 aaggtgaggt tataagacga agacctgaat ttgaaatgga acatagactc ttgcttaata
     6961 tgataaacta tgatgaaggt acgattaatt taaaaggtaa aacatataaa ttaaaagata
     7021 cttatttacc tacaatagac aaaaaagacc catataaatt aactatggaa gaaagaaatg
     7081 ttattgataa attagtatct tcatttagag gtagtgaaaa attacaaaaa catgtttcat
     7141 ttttgttttc taaaggaagt atatatttaa aggctaactc aaatttactg attcatggat
     7201 gtgtaccgtt aaatgaagat ggaagcttta tgtctatgaa tataatggga aaagagtata
     7261 aaggtaaggc tcttatggat aagatggagt ctctagcaag agaaggattt ttctttaaag
     7321 ataaggcaga agaaaaactg tatggcatgg atattatgtg gtatttatgg actgggaaat
     7381 gttcatcatt atttgggaaa gatgatatga ctacatttga aagatacttt atagctgaaa
     7441 aagagactca taaagaaaat aagaacccat attttaaact tagagaaaat gagatggctt
     7501 gtaaaagatt atttgaagaa tttgatttag aacttgatga atctcatata ataaatggtc
     7561 atgttcctgt ggagagtaaa aatggagaga gcccaataaa agctaatggt aaaattctcg
     7621 ttatagatgg tggtttttct agggcttacc aaaaaactac tggtatagct ggatacactt
     7681 tgatatacaa ttctagaact ttgcaattag tatcgcatga accatttaat tctgctgaag
     7741 aagccattgc aaatgaaagt gatatcttat cgacaactgt tgtagtagag cataaggcaa
     7801 agagaaaaat ggtgagagat actgatgaag gagtaaagat acaagaggaa atagaagatt
     7861 taaaactact tttaatggct tataagaaag ggctaataaa agaaatgtag atgttataga
     7921 ggcacttcat gattgaagtg tctttttgtt aatacttatt aaatatatta taaagattaa
     7981 taatagtagt ttatattata cttactaagt gaatccaaga taaatgggtt cacttttttt
     8041 atttataaac attattaaaa aaatatagat gttttggtta tattccttgt cctttataaa
     8101 tagaattata taaacaaagg aggaggacaa gagatgaata aactttctga tgaaataata
     8161 aactctcttt ctacaactat aagagaaaag gtagaaaaag tctcaaacaa taatctgaac
     8221 atagaagaaa ttaggttacg ctcacaaaaa ccattgatac taaatgcaaa ctctaaagat
     8281 tatttttaca atcaaaaaac tatgacactt gatttaaatc aacaaaactc atatgtagta
     8341 acaagagaag atgtagagca aacttttcaa ataatatgta aatattcaat acattcattt
     8401 atggatgaca taaaaaaagg attcataact ttgagaggag gtcatagagt agggttagtt
     8461 ggaaaagcaa tagtagaaga tggacaagtc aaaaatataa agcatatatc atctttaaat
     8521 ataagggtat caagggaaat tattggatgc tcggataaga ttttaagtca tatcataaag
     8581 ggaaaaaatc agattaataa tactttgata atatcaccac ctcaatgtgg aaaaacaact
     8641 ttaataagag atatagtaag aaatttaagt aatggtaatg aagattatgg atttaaaggt
     8701 ttaaaagtgg ctttagtaga tgagcgtaat gagatagcag gagcttatct aggtgttcca
     8761 caaatggatg tgggaataag gactgatata atagaaactt gcccaaaaga cttgggtatt
     8821 acaatgcttt taaggtctat gtcaccaaat gttattgtga cagatgaaat tggaagtgaa
     8881 aaagaaataa aggcattata cacagcatta aatggaggta taggattaat aactactgtg
     8941 catggagatt caatagagga tatacaaaat agaaaagaat taaatagact tctggataaa
     9001 gagttattta aaaaggttat aattctttct gcaaaaagag gagcaggaac aattgaaaaa
     9061 atttatgatt tagaagaaaa gagatggtat tttgcaaatt aaaataatta ttatagcttt
     9121 cttgatagga agtagttatt tgataggtga acaaatatac aaaacttata ctcgtagaca
     9181 taaacaatta aatgatttaa taagagtttt agaaattttg aggatggatt tatcttttgg
     9241 actttataca ttagaagaaa tctttaatag gataggtgga aataaagagt tttgtttttg
     9301 gaaatttttc tatcaaatat cagaaggact tcacaatgaa caaagtaaaa ctttggaaat
     9361 aataatatca gaaaatattg atgtcttaag caaagaaact tatctaggca ataaagaaat
     9421 tgaggaactg aaaaatctaa tacttacttt agggaaaagc gatatagagt cacaacaaag
     9481 aatgatagat ttatctatag aaaatttgaa aaaacaaacc tatgagacaa aggaagatat
     9541 aaacaaaaaa ggcgtattgt ataaaaaatt agtgacattc ataggtatag gtatatgtat
     9601 tatattgatt taatatgggg aggtttatta tggaaatatc attgatatta aaggttgcag
     9661 gtgtcggtat tctaatatca gtattgaata tgatattgga aaaaacagat agaaaagatt
     9721 gggcaggtct tactacatta gcaggtgtaa taatcgtatt gggtatggta ataacagaaa
     9781 taagtgactt gtttaatact gtaagaacaa tgtttcaact ttactaaaga ggagggagta
     9841 atttggaaat aatgcaatta ataggaattg caatcatttc aactacactc tgtttagtaa
     9901 ttaaaaaaga tagaccagag atagcaaatt tcatagctat aataactgga gtaataatac
     9961 tattgtcagt tatgtttaag ctaaatttta tagtagatag tatacaagat ttggctaaca
    10021 aggctaacat cccgactatg tatatatcat taataataaa attaattggg atagcatact
    10081 taatggaatt tgcgatacaa ctttgtaaag attgtgggga aggaaacatt gcttcaaaac
    10141 tagaatttgg aggtaaaata atagttatgt caatgtcttt ccctatactt ttgtccattg
    10201 tagaaatggt ggtgaatata attccataga tgtgtatctt ggaatgaata taagtatatt
    10261 agagagcttt cgttgtattg aggtttatag agtgaagaga ggcatggttg cttatgaaaa
    10321 aaagattttt accaatgatt ataggttttt tatttacttt cttttttgta aatatctttg
    10381 ctattatgat atttgctaat gaagtaccaa gtagtgaaga taaagaatat ggtgaaacaa
    10441 aaaatagcat agataagtat atagatggtc aattagacaa gctagatata aatgagattc
    10501 aagactatat aaataaagaa atagttatta atgatgttaa tttaaaatca tttgtaaaag
    10561 atttaattag tggagaaaaa aatatcttag atttattcaa taaagatgga ttgaagatat
    10621 tgatgtttga tgaatttaaa gcaagtttaa aggtagtagc agtaattttg gtactggctc
    10681 tgttatcatc tattttaaag agtctggaaa actctttttc atcaggtgca gtaagtcaaa
    10741 tagcaacata tataatattt atcacaatgg tatcacttac acttattggt tttaaagatg
    10801 tattacagat ttgctatgat gcaatagacc atactgtagg tcttatgcaa gttataatgc
    10861 caatattaat aacattttta cttttaattg gattcccaat aacgtcgact actttgaatc
    10921 ctatattcat aggaggagta acatttataa atgtattttt taaaaatttt ctatttgtat
    10981 ctattactgt tgcatttgga attttaataa taaacaattt atcaaaaaat ataaggctaa
    11041 agagattttt ctcctttgta aaacaaataa attatgtttc tattggagct atgtttactg
    11101 tttacttagg tcttgtatcc atacaaggat tgtatgtaac cagttttgat aaattcagtg
    11161 taaagactgc taaatttgct ataggtaatt ttattcctgt tgtaggtgga tttgtatctg
    11221 actctgtgga tattttatta tcttcatctc agcttataaa aaatatattt ggaggaatag
    11281 gacttatctt acttgttgga atttgcttac ttccagtaat aaaaatttta tcagtaatag
    11341 tcgtgtacaa attagcagca ataattgtag agcctgttgg agaggatgga atatctaatt
    11401 ttttaaatga agtggcaaat ttaatgataa taatgctagc atcagtaatt gctataacag
    11461 taatgttctt tgtaactgta gctatcttga cctcgataag tgtcgtaagt caagggtaat
    11521 ttatatatca gtgaaaaata attacattta atctaatgtt tttagagagg aggatgctat
    11581 gttagagggc ataaaggcat ggatagttag tgttttgata ggagcattta tagtaaatat
    11641 agtagatatg attttaccaa gttctaagat aaaaccttat gtcaacctag ttttaaactt
    11701 tatgtttgta tttattgtaa taactccagt agtaggattt ttttcaaaag atatgagttt
    11761 agaagataga atattaaaat ctatgggaaa ctacaacaag cagtatgtag atagtacaaa
    11821 tgctttggca aaagagactg gaaataatag tctatccaag ggatatgagg atgggttaaa
    11881 agaagtactt aaattaaaac ttgatgaata tggttatgat ttagaagata tagagcttaa
    11941 tggggcaaat ataaataata taaaaataaa agaaaagaat aatagtgcaa aaagtaataa
    12001 cagttccaat gaagaaaata aaaataatag tactaaaaga gaggaagaaa acataaactc
    12061 taatgacaaa gaaaattcta aacaagtctt caaaaaaggt acagagtatg ggttgaacct
    12121 aaatgaagaa aaattaaaaa acgacttaat aaaggtgctg gatgtttcta tagaagatat
    12181 acaaattgat aaataggagt tgggagaatg ttgaaaaact taaatgacaa agataagaga
    12241 aaggtatatt cactaataac tatagcagga atatgtgtgg tttcattagt attattatct
    12301 tgttttccta gtagcaaaag cgataaacaa gttggaaaaa cagagactaa taaaaatgct
    12361 gaaaagcaag tcactaaaga acaagaaaaa gatgatttag agtcaaaatt aacagcaata
    12421 ctatctaaaa ttgatggtgc aggagacgtt gatgtaatgg ttacatttga atcaagtgaa
    12481 gaaatacaac ctgcttttaa ttcaaataat acaacagaaa caacagagga aaaagatgca
    12541 caaggtggag aaagaacagt tacgacttct agtgaaaata aaacaatgat aacttcaaat
    12601 tcaagtgacc ctgtagttat aaaaactaca gagccaaaga taaagggagt gatagtggta
    12661 gcaagtggag caagtgaccc aagtgttaaa gagacacttt atagtgcagt acaaacatca
    12721 ttacaagtag ctggtcatca agtagaaata tattctaaat aaatacatat aaccgggggg
    12781 ataaatgaat gaagtttaat tataagggaa gaggatttgt aataataact ttaactgcaa
    12841 tgttagtggt agtaggaaca gtgaattatc aactaagtaa gaaatcttta ttagaaacat
    12901 caaaagagtt taaggcatat gaagaagcac agttgcaaaa aaatactgat gagagtgata
    12961 gtagtaagac agaggattct agtaatatag ataaacaaga tggtaaggaa agtgctgata
    13021 ttgatatagt cgatagcaaa gctagtaaag taaaagaaaa ggcaactgag acaagtaagg
    13081 aaataaaagc tcaattatca tcagagaaaa atatgaaaaa ggcatcttat attttagata
    13141 tgaaaatgaa tagagaaaaa caaagaaatg agttagttca agatttaaat gaaatgataa
    13201 ataatccatc tacaacagaa gaatctagaa aagaagcttc taatatgaaa ttaaatatag
    13261 ttaaaattca agaaaaagaa cttcaaatag aaaacctttt gagtacaaaa ggatatgaag
    13321 aagcgttagt ttatataagc gataacaaag ttaatgtagt tgtaaatgaa gctaaattag
    13381 aaaagaaaga tgcagcaaaa atatttgatt tagttgcaga acaagccaat gtgaagtatg
    13441 aaaatattaa acttacaaat aataatagta ataagtaaag tataaacctc agaattaact
    13501 gaggttattt tcttgcaaag tagacttgta tttggtatta ttatacttat aaaaatagtt
    13561 ccttaaaatc aggaggtaag ttttaatatg gaagataata aatttggaca agtaaaaata
    13621 tctaatgatg taatagctac aatagctggt ttagcagcat tagaagttga aggcatagaa
    13681 actacagcaa cattaacaga taagttgtta aaaaataatg gagtaaaaat acaaatcgaa
    13741 gaagaggatg ttaatttaga tgtaatggtt acgataaagt atggaatgtc aataccagat
    13801 acagctttta aagttcaaga aaatgtaaaa aatactgttg agacaatgac aggactgaag
    13861 gtttctcaag taaatataca cattcaagga atcagcttta agaaggataa agttgataaa
    13921 gaagaagcaa aagcaactaa gaaaaattaa tattagccct ctgaaaagtc agagggtttt
    13981 ttgctggtaa ttgattttat atggataatt aaacttaaag gagaattttg attgatgaaa
    14041 aaagataggg cacaaaagag tacaacaaga gaatatataa tgaaacttat ataccaaata
    14101 aatattaata aagaagattt tgaaacttta gaagataaag tagataattt tttaaaagat
    14161 aattcagaac atattataaa tagatataaa gaacttgcac ttcaatactc aaaaaataca
    14221 aatttaaagt tagaagatac tgaaattgaa gatgttatag acaaaaaata tataaataca
    14281 gtgtgcaaag cattgaaaga aaatcatgac aaaatagatg agttaataaa taagcatgct
    14341 aaaaactgga cagtagatag aatgccaaag gtagatgttt ctatacttag attgtctgtg
    14401 tgtgaaatac tttatttaga cactcctaat aaagtatcaa taaatgaagc agtagaactt
    14461 gcaaaaatat attgtgatga taagtctcct aaatttataa atggtatatt gggaagtgtt
    14521 gttgatgaaa ttgggaaata atataataat tggaattgat actagctgct atactacttc
    14581 tatagcagct atctctttag ataagaaagt tatattcaat gaaaaaataa tgttagaagt
    14641 aagggataat tcaaaaggtt taaggcaaag tgaagctgta tttcaacata tcaataattt
    14701 agggattctt agtgatagga taaaatcttt taaagataag tttaatgtag aaggcgtttg
    14761 ctcatcaaaa aaaccgagac ctgttgaaaa ctcatatatg cctgtattca atgtaggtca
    14821 taattttgga aaattattgt ctagtatata tggttgtagg ttttatgaga ctactcatca
    14881 agagaatcat attgaagcaa gtttattaaa tagtaaatta aaaaataata ataaatttat
    14941 ttctgttcat atgtctggtg gtactacaga gatactttta acaagcaaac aagatagtca
    15001 tcacaatgta tgtgatacta atttaggtaa aattgccaaa ataagtatta aaaaagatga
    15061 taaaagtaag ttatacaata attttggata taatatagat ataattggag gaagtaaaga
    15121 tattagcttt ggtcaactta tagatagagt aggaattaag cttggatata aatttccttc
    15181 aggaaagtat ttggatgaaa atgctttaaa ttgtaatctt aaaatagaaa gtggtctgaa
    15241 aacttctgta agagatgggt atatgaattt atcaggatta gaaaatcagg taaataagat
    15301 tataaatgat aatggagata atactaatca aaaagaatat atatctaaat tggtattaga
    15361 ttcagtagtt agaaatatgt ttaaatcttt agtgtattta tgtgaaactt ataatgtaaa
    15421 tgaagtagta tttgctggtg gagtttctgc aagtaagtat attcttagag aattaagcat
    15481 gaagttaaga aaaaagcata tagaagctta ttttacagaa ccacaatatt caactgataa
    15541 tgctgtcggg tgtgctataa taggattgaa taacttttta ggagaaagag tatgaaacta
    15601 agagctttag atataagtga agcaaactca tatattaaaa gaattttaat aaacgaccca
    15661 atactttcta atcttaaagt taaaggagaa atttctaatt ttaaggttca tagtagtggg
    15721 aatgtatatt tatcacttaa agatgaaaca tcaaagttaa actgtgtaat ttttaaaagt
    15781 aattttaatc gcaatttaaa attagataat ggtgtaaaaa ttatagctaa tggatatata
    15841 tcagtctatg aaagagatgg tgcgtatcaa ttatacatta atgaaattga aatagaggga
    15901 ataggaaatt tacacataga gtttaataga ttaaaggaaa aacttaataa agaaggatta
    15961 tttgacccta aatacaagat acctatacct aaaatgccaa actcaattgg cgttataaca
    16021 tctccaacag gtgctgttat aagagatata ataaatgtaa taaaaagaag atatccgaaa
    16081 gttaatataa agctttatcc agtaacggtt caaggagata aatcagctga agaaatctgt
    16141 gaagcaatta gatttttcaa tcatatgaag aatgttgata cacttatagt tggtagaggt
    16201 gggggttcga tagaagaact ttggtctttt aatgatgaaa tggtagcaag agaggttttt
    16261 aattctcaaa ttccgataat atcagcagtt gggcatgaaa cggattttac tatttgtgac
    16321 tttgtgtctg atatgagagc cccaacgcca tcagcagcag ctgagattgc tactccatca
    16381 ttggatgata taaactataa acttggaaac ataaaaagta ggatgagtaa gtctcttaca
    16441 aatcaaatag agctagacca atataggtta gaaacagtat ttaataagat aaataactat
    16501 ttagattcgt acacaataaa agataaagtt atacaattag acaaaatata tgataaaata
    16561 atttttggaa tagaaaataa tttaaaatta gaagatgaaa aattagtaaa aattggagca
    16621 ttactccata atttaagtcc attagctact atggatagag gttatagtat aactcaaaaa
    16681 aatggaaaag taataaatag tataaaagga ttaaaaataa aggatagtat agatatagta
    16741 ttaaaagatg gaaatttaga atgtatgata gataaaattg aaaataaaga gggatagtat
    16801 gaatttaacc tacgaagaag cttataaaag attagaaagt attttaagtg aattagaatc
    16861 taaaaatgct agcttggatg aatctttaag cctatatgaa gaaggtataa gcctttataa
    16921 acattgtaat aagcttttag atgatgctaa attgaaaata agtaaattta atcaattggg
    16981 aatagaagaa gactttaaaa tagaggagga ataaaattgg aatttaaaca atgtctaaaa
    17041 gaaaaagcaa gttttgtaga aaaagtactt aaagaatata tgcctaaaga agaagggtat
    17101 caaaaaactg ttattgaagc tatgaactat agcttaagtg ctggaggtaa aagattaaga
    17161 ccaatactta cattggaggc ttgtaaaata gttggaggta atgaagatga agctattcct
    17221 tttgctattg caattgaaat gatacataca tattcactaa tacatgatga tttgccagct
    17281 cttgacaatg atgatttaag aagaggaaga ccaacaaatc ataaagtata tggagaagct
    17341 atggggattt tagcaggaga tgctttgtta aactatgctt ttgaagtaat gcttgcaggc
    17401 tcaatcaata aagaaaatcc agaaaagtat ctaaaagcta taaatgaaat tgccaaaggt
    17461 gctggtatat atggaatgat aggtggtcaa gtagtagatg tagaaagcga aaataaacaa
    17521 attgaaaaag aaaaattaga ttatatacat atgaataaaa ctgctgcaat gatggtaggc
    17581 tgtatgagag caggagctac tataggtgga gcaaattctg aacaaatgga agaaataact
    17641 aaatatgcaa aaaatatcgg attatcattc cagatagtag atgatatact agacatagta
    17701 ggagatgaag ctaagttagg taaaaaggtt ggaagtgata tagaaaatca taaatcaaca
    17761 tacccatctc ttttaggttt agataaatct aaagaaattg cacataattt gattgatgaa
    17821 gcaaagaaaa gtatagaaaa attatctgat gatgtagatt ttttaaaggg attggctgaa
    17881 tatataatag atagagaata ctaaaatata cagctaaaat cgaggaggga aaaatggatt
    17941 ttttttcgga gatttttaac aatggggctt tgggaataag tctgattgct tgtttcttag
    18001 cacaatttat aaaaatattt actggtaagg aaaaaagaat tgagttatca agaatactta
    18061 tttcaggagg tatgcctagt tcccatagtt cttttgttac aagtttagct acagtggtag
    18121 gtattgaaaa agggtttaat tctactgatt ttgctataat taccgtgctt gccttaatta
    18181 taatgtatga tgcagcaggt gttagacggg ctgttggtaa acaagctact atattaaatc
    18241 aaatggtggc agatatacag catggaaagc atatagagca gaagaaatta aaagaattaa
    18301 taggtcatac accactagaa gtttggtttg gagctttact tggaatagta acagctttaa
    18361 tattgatgta aattgaaaat ttacagaata aaggtgagga taatatgtat aaatatttag
    18421 ataaagtaaa ttctccaaag gatataaaaa atatgagtat agaagaaatg gatttacttg
    18481 caaaggatat aagaaagttc ttagtaaaat ctgtttcaaa gacaggagga cacttagctt
    18541 ctaatctagg agtggttgag ttaacattag cacttcataa agtatttgat agccctaaag
    18601 ataagattgt atgggatgta gggcatcaat cttatgttca taaaatagtt actggaagaa
    18661 aagattgttt tgtatctttg agacagttta atggactaag tggttttcca aaggagaatg
    18721 aaagtcctca tgatattttt gatacaggac atagcagtac atctatttct atagctacag
    18781 gtattgcctg tgctagggat ataaaaaaag aaaactatag tgttatatct gtgataggag
    18841 atggttctat aacaggaggt atggcacttg aagctttaaa tcaattagga tatatagata
    18901 ccaatatgat agtaatcctt aatgataatg agatgtccat agataaaaat gttggaggaa
    18961 tgtctaaata tctgtcaagt attataagaa actcaacagt tgagaaaatg acagatgaag
    19021 ttgataaaat tttaaatgtt acacaaacag gtgaaatttt atctaagact gcacatagat
    19081 ttaaagataa actaatgtat agcttttctc ctcaggactg ttcatttttt gattcattag
    19141 gtataagata ttatggacct atagatggcc ataatactaa agaactaata gatatattga
    19201 gaaaagctaa acacaaaaaa gggcctgttc ttttacatgt aattacgaaa aaaggaaaag
    19261 ggtacagatt tgcagaagaa caacctgata aatatcatgg agtatcaaag tttgatataa
    19321 agacaggtgt tacatctgca aaagttaagt ctatgtcaat tagtgttgga gaaaaacttg
    19381 ttgatatggc taataataat gaaaatatag tagcaataac agctgcaatg ccatctggta
    19441 cagggttaaa cctgtttgaa agtgcatatc caaaaagata ttatgatgtt ggtatagctg
    19501 aacaacatgc aacaggattt gcagcaggtc ttgcaaaaaa tggtatgaaa ccttactttg
    19561 ctgtgtactc atcatttttg caaagggcat atgaccaagt tattcatgat gtatgcataa
    19621 caaaaaagcc agttactttt cttatagata gagctggatt agttggaaat gatggagaaa
    19681 ctcatcatgg tatgtttgat ttaagttact taaattctat tccaaatatt gtagtaatgg
    19741 ctccaaaaga cacaagagag atggagctta tgatggattt gtcattaaaa ttagattgtc
    19801 cactagcaat tagataccca agaggaagta gttattacct agataaggga gagtatggag
    19861 agattgtatt gggtaaatat gaggtattag atgacggtca agatacagtc atactttgta
    19921 ttggaagtat ggtaaaacat gctttagagg ctaaagaaat attatcaaga gaaggaataa
    19981 atccgacaat agttaatgct agattcttaa agccaattga tgaaggtatg ttaaaagcat
    20041 tattaaaaaa tcataaaaat gtagttacta tagaggataa tattgtaact ggaggatttg
    20101 gaagtagaat aaataagttt attatagata atgaatataa cgtaaatata cttaatatag
    20161 caataccaga agaatttgta aaacatggca atatagatga attatatgat tttgtaggat
    20221 tatcgccaaa aagtattgct gataagataa ggaaattagt tattgagtaa aaatctaatg
    20281 taaatattgt aaattaaaat ttatataaaa tatagaattt atcaattaga ataaatatca
    20341 agtgtgttac ttcaagctac tttagttatc attaaagtag cttttaacag aaaggtaatt
    20401 gtatgaaaaa aagaatagat ttattattag tagaacaagg gtactttgaa agtagagaaa
    20461 gagccaaaaa agcaataatg gctggattgg tttttgttga taatcaaagg tgtgataagg
    20521 ctggtacaga agtaaaagaa gatagtagta tagaagtaaa aggaaatcca ataccatatg
    20581 tcagtagagg tggtttaaaa cttgaaaaag caatgaaaaa ctttgatttg actattgatg
    20641 gaaaggtatg tatggatata ggggcatcca caggtggttt tacagattgt atgctcaaaa
    20701 atggagcaat aaaagtattt tctatagatg ttggttatgg tcaacttgca tggaaattga
    20761 gacaagatga tagggttgtg tgtatggaaa gaaccaatat aagaaatgtg acaatagaag
    20821 atacaaagca gtttgctgat tttgcgtcaa ttgatgtgtc ttttatatcg ttaaagttag
    20881 tacttccaaa ggcaaaggag ttagtacgtc atgatgggga agttgtcgct ttaattaaac
    20941 ctcaatttga agcaggaaga gaaaaagtag gtaaaaaagg agtagttaga gaaaaatcta
    21001 cacatataga agttataaaa atgatttctg atttctcagt cgaaaatgga tttgagattt
    21061 tagggttaga tttttctccc attaaaggtc ctgaaggtaa tatagaatat ctaattcatt
    21121 tgagaaatgg aaatgaagga tatgaatttg atggtgagac atacaataac aaaatagtag
    21181 aagttgttga agcttctcat aatttagaca aataggttaa aagggggctt agatttgatc
    21241 ctcgaattat atatgaaaaa ttgtgcctta gttgaagaac taaggttgaa tattgataaa
    21301 aacttaaata tacttacagg tgaaactggt tcaggaaaat ctataataat agatgcactt
    21361 ggactttgtt taggtgataa atatgatagg tcttttttaa gaaaaggaac agataaggga
    21421 cttgttgagg ctgtattttt ttcagacaat agatatctta aaaaaatatt agaagaaaat
    21481 gatataagta tggaagatga taatttattg gtaataacta ggttgatata ctcagatggt
    21541 aagagtactg ctagagtaaa tggaaggact gttaaagtgt ctcttcttaa agaaattgca
    21601 tctacactta tagatataca tggtcaacat caaaatcaag ctttgtttaa taaagatact
    21661 catttaaaat ttctagacct ttttggagaa aatgaattgg aagagtttaa aattgcttat
    21721 aaaaatgtgt accataagta tagtgaagta aaaaaagctt taaattgctt gacagaaaat
    21781 aaagatgaga tgcaaattca acgagaaata gatttattaa gatttcaaat aaatgaaatt
    21841 gaagctgcaa atctaaataa gaatgaatat gaagatttat taaaacaaag agaagtttat
    21901 agaaatagtg aaaagatata taataatcta aattcgagtt atagcaagtt acataatggt
    21961 gagtataatg ttattgacct aataggtctt gcatctaagg aacttaatga catatctaaa
    22021 tatgacagtg ttttaagtga atatagtgat actgtagaaa gaatcatgta tgagttacaa
    22081 gacatatctg gtgagataag aaattacaaa gataatatag actttgaacc ttatgagcta
    22141 gaacagattg agcttagaat cgacgagatt aataacttaa gaagaaaata tggagacagt
    22201 atagatgata tatttgagta ttatggaaaa attaaagata gattagatga aatattaaac
    22261 agagatgaaa gagttgaaca gttaagaagc aaactgatga atatagaaga agatttaaaa
    22321 ataaaagcca gtaaattgac taaagcaaga atagaagtag caactacttt agaaaaaata
    22381 cttcttgatg aattaaaaag cttaaatatg aaaaatgtaa tgttcaaggt taattttgaa
    22441 aaaggttcat ttactttaga tggagtagat gatattgaat ttatgatatc ttttaactta
    22501 ggagaagata ttaagcctat atataaagtt gcatcaggtg gtgagatgtc cagatttatg
    22561 ttagcgttta aaaccatatt ggctgatata gatgatattg atacattagt atttgatgag
    22621 atagatactg gaataagtgg gatagcagca cagatagttg gtgagaaatt gagtgatata
    22681 gcaaagaaaa aacagataat atgtataact catttacctc aaattgctgc aaatgcagac
    22741 actcattatt gtatagagaa agatacttca aataatagga cgtttaccaa tgttagtaag
    22801 ttaaatgaat ctcaacgaaa aaatgaaatt gccagactta tagcaggtaa caatataaca
    22861 gaaaaaacta ttgaacatgc aagtgaaatt attgagttgg ctaaaaaatg ttagtgaaaa
    22921 acaagcattt taataggtgt aaagaatttt tatagaaatg ctttacatct attttttatg
    22981 atgaaattat tttaaaatat ctagtgccta tattgaagaa aagtagattt aaaaatagaa
    23041 gaaaatttgt ataaatttgt aactttgaaa tttctaaatg cttaagtata atattattta
    23101 ttaaggtagt agtgaggggg acataaaatg aataaaaaag atactatgca cttaattttt
    23161 acaattgtaa tttttatttt attatggtat attgtggtag cggtaagtga gattaataaa
    23221 aatattgaaa gttgtagtaa taaattagat tatttgtcac aagagattat gagtactaaa
    23281 tcggatatta gtaatactat aaatgaagaa atgtcaaaaa gttatataac aaagagtatt
    23341 gacttaaaag taaaaaaaat cgagaaaaaa gagtgtgtta ttgatgtaga tgttcaatta
    23401 agtaaacttg gaaaaaatgg aaaagtattt tttatgtata aagaagatag tgataaatgg
    23461 aatgaaactg agatgacaaa acatggagaa ttatcatatg catgtgaaat aaagataaat
    23521 acaggaagtg aatatgacta taaagtagtt acaagtggag ctatatctga aagttctgat
    23581 gtgcaaaaat ttactaaatc tgattatatg cctgaacaac ccatatttaa ttctgggata
    23641 agagataata gagaatactt tattgaaata gtagaaaata gtaatctttt caatcatgag
    23701 agtgaaaagt caaaaaataa agcttacgat aatattagat tagaaaaagt agatattatt
    23761 gtaaatgaag gaaaaaaaga cactatatac aaagccaaat tagctgaagg tttagatgat
    23821 ggtaaaacta ataattcaaa tagaaatcaa gtgaattatg aagtaagttt tcctaaaagg
    23881 gatattgatg acataattta tattaaagct aaacttacat acaataatgg agtagaatat
    23941 acaaaggata t
//
LOCUS       DAEMHW010000045        23420 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEMHW010000045 DAEMHW010000000
VERSION     DAEMHW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.045"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(122..1078)
                     /locus_tag="KPK67_001530"
     CDS             complement(122..1078)
                     /locus_tag="KPK67_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBG2458518.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
     gene            complement(1435..2133)
                     /locus_tag="KPK67_001531"
     CDS             complement(1435..2133)
                     /locus_tag="KPK67_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2458519.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            complement(2167..4869)
                     /locus_tag="KPK67_001532"
     CDS             complement(2167..4869)
                     /locus_tag="KPK67_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBG2458520.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            complement(5047..6864)
                     /gene="ftsH"
                     /locus_tag="KPK67_001533"
     CDS             complement(5047..6864)
                     /gene="ftsH"
                     /locus_tag="KPK67_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG2458521.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            complement(6895..7338)
                     /locus_tag="KPK67_001534"
     CDS             complement(6895..7338)
                     /locus_tag="KPK67_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2458522.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            complement(7831..8721)
                     /gene="metA"
                     /locus_tag="KPK67_001535"
     CDS             complement(7831..8721)
                     /gene="metA"
                     /locus_tag="KPK67_001535"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBG2458523.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(8751..10016)
                     /locus_tag="KPK67_001536"
     CDS             complement(8751..10016)
                     /locus_tag="KPK67_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBG2458524.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(10126..10228)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(10282..12939)
                     /locus_tag="KPK67_001537"
     CDS             complement(10282..12939)
                     /locus_tag="KPK67_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG2458525.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTILQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQCSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     gene            complement(13067..13810)
                     /gene="yaaA"
                     /locus_tag="KPK67_001538"
     CDS             complement(13067..13810)
                     /gene="yaaA"
                     /locus_tag="KPK67_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBG2458526.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMAKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            complement(13820..14284)
                     /gene="bcp"
                     /locus_tag="KPK67_001539"
     CDS             complement(13820..14284)
                     /gene="bcp"
                     /locus_tag="KPK67_001539"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBG2458527.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVARATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            complement(14636..16333)
                     /gene="ade"
                     /locus_tag="KPK67_001540"
     CDS             complement(14636..16333)
                     /gene="ade"
                     /locus_tag="KPK67_001540"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBG2458528.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKIATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIASLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            complement(16639..17793)
                     /locus_tag="KPK67_001541"
     CDS             complement(16639..17793)
                     /locus_tag="KPK67_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBG2458529.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            complement(18038..18877)
                     /locus_tag="KPK67_001542"
     CDS             complement(18038..18877)
                     /locus_tag="KPK67_001542"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBG2458530.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            complement(18890..19486)
                     /locus_tag="KPK67_001543"
     CDS             complement(18890..19486)
                     /locus_tag="KPK67_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG2458531.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            complement(19537..20187)
                     /locus_tag="KPK67_001544"
     CDS             complement(19537..20187)
                     /locus_tag="KPK67_001544"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBG2458532.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            complement(20233..21486)
                     /locus_tag="KPK67_001545"
     CDS             complement(20233..21486)
                     /locus_tag="KPK67_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2458533.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            complement(21510..22151)
                     /locus_tag="KPK67_001546"
     CDS             complement(21510..22151)
                     /locus_tag="KPK67_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG2458534.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLTKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            complement(22353..22766)
                     /locus_tag="KPK67_001547"
     CDS             complement(22353..22766)
                     /locus_tag="KPK67_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBG2458535.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            complement(22917..23258)
                     /locus_tag="KPK67_001548"
     CDS             complement(22917..23258)
                     /locus_tag="KPK67_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBG2458536.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
BASE COUNT         7406 a         4156 c         2472 g         9386 t
ORIGIN      
        1 aagaaaatat aattgtcaat attttctata aatattttca atttaaaata tttttttatt
       61 tacttaaatc agcttctcaa ttgatgtatt tttataaaat caattaaaca tcatatgtaa
      121 tttacaataa tttttttatt ttaaatatta taattccaac aaaaattata catagcataa
      181 aaaataaagt tgctggatat ccataaatcc agtttaattc tggcatatat ctaaagttca
      241 ttccatatat accagttata attgtaagtg gtgctgatat ggctgttaac aaggttaatt
      301 ttgtaattaa gttatttgtt ttctcaccta ctctggaaga ataaatatct aataatttat
      361 ctgctaactc tcgtgttgac aatgcgaaat tgtataattt gtcaattcca aaatctatac
      421 cttgaaaatt ttctaaatta tattttttta catttgaata ttttaaatat cttatatttt
      481 ccttaactat tctatcccca atatacaaaa gaggtctagt attcttaact ataattcttg
      541 ttaatcctct tacatcactt attctagata catgattctt attaatgttg tccatcattt
      601 catcttctat ttgaagaatt aactcttcaa ctttttccaa agactcaaaa ccatttttta
      661 taacttctct aagaatcaag tagtttatct taaatagatt aataactgga tttttttcaa
      721 gttgagagtt cttcaaaata atatttttta caaattcaaa taaaaagtgt tcttcattca
      781 ctacaaccaa tataaaatta tctgataaat acatatttac ctcttctata acagcttctc
      841 catctcttag ttcaaatgta tttaggctta aaaaatcgta attatcaaat aaatcaagtt
      901 tggttatttc atcaaattta agacaatcat taaaagttat ttcatctata tccaaagttt
      961 ccttaagcaa ttttaaattt tttggagcac ttaatataag ataagaattt tctgccacat
     1021 aaaatttatc tctaaaatct tcaatagtct ctcttgtatt taaattcagt atatacatac
     1081 atctactcct aaatttcaat ttacattcta aaaatttttt ataatcctta ctgtaattat
     1141 tttacactat tttaataatt attatttaat atttatatta aatttattat aaggtaagtg
     1201 ttaaatacat ttattattta accaaagttt ttattacaga ttacactata tacttatttt
     1261 aaaacatatt tcatattatt gatactaaaa aattattgat actaaaaaag ctaaaaaata
     1321 aaatgcatgt gctaaactga caacaagcac atacacttca ttcttatttt aactaaacta
     1381 tataatttta atatatatta ataagcacat aatctaataa ttaatatatt acaactattc
     1441 atcatttaat ctgtatccta caccaacttc tgtaattata tatcttgggt ttgcaggttg
     1501 tttttctatt tttcgtctta acgttgccat aaagaccctt aaagacttag tctcattccc
     1561 tataacacta ccccatattt cttttattat aaaattatga gtaagcactt ttccatgatg
     1621 ctttgcaagt aatgaaagaa tattatattc aataggtgtc aaatggactt caacatcatc
     1681 aacaataact tttcttttat caaaatcaat caataattta tctacttcaa acgtagattt
     1741 aacatcatct ttttgttgag cttctacttg tgaatgtcta aatgctactc ttactcttgc
     1801 taataactct actatggaga atggttttgt taagtaatca tctgcccctg catcaaatac
     1861 ttctaccttg tctctatctt gctctctagc tgaaactaca attattggta tatttgaaaa
     1921 ttgtctgata gcttttatta cctctattcc atccatatct tctaagccta aatctaaaag
     1981 aacaacatct ggagaatatg agacagcaat agatatacct tcttttccag ttatagcctc
     2041 cttgacatca taatcctgtg ttaaaagtgc agtagatata aattttctta ttgtcttatc
     2101 atcctctact aacaatataa gttttttact catttcttcc tccaaaacta attttaaaat
     2161 ttaaaactat tcattttcct taggcaaact aaacttaaaa gttgaaccct tatctttatt
     2221 gttttctacc ataatttcac ctttatgagc ttgaattata gatttacaaa ttgataatcc
     2281 aagtccaaca ccctttctgg aatctttagc tccttcttct ccagtaaaaa atctgtcaaa
     2341 gatatgcttt ttaagttcct ttgatatacc aggaccatta tcagatactt caaaccaaac
     2401 ataatcatct ttctcatata catttatctc tatcttacaa tcttcctttg aatatttcaa
     2461 agaattatct attaaattaa ttaaaacttg ttctattaat tttgcatcca taggaacaaa
     2521 taatatctgt tctggtatat taatatctat aagtgcattt tcaattcttt tttttatttt
     2581 ttgtaacgct tctgatataa tttcctcaac cagttcagaa tgttttttaa cttccaattt
     2641 cccttcatct atcttggtca tactaagtaa attttcaaca agtcttatta accactgagt
     2701 atcttcatat acaccactaa gtaattcatc tattatatct tgacctatag tacccttgtt
     2761 ttttataatt gtacttactg caccagatat tcctgctagt ggagttctaa ggtcatgcga
     2821 aactgctctt aaaagattac ttcttaatct ttccctttca atctcaagat tggtattttc
     2881 tttagcttct gacaagattt ctctatcaag cgctattgcc atttgagcaa ttattgtttc
     2941 aattaagctt atatcttctg tatccaattt tgaatcaata catgatattc ctactactcc
     3001 taaagttaaa ttcattccta caattggtat atagtatccc tttgaaccag gaagtgtatc
     3061 cgtgtcattt cctgctactg ttgaattgga taaaacccaa cttgcaacag ctttttcatc
     3121 ttcactatcc aaatcatttt tatccttatt attagtattt tttttgtaaa gctttgattt
     3181 attatgctta tctacattta aataacaaat tacatctcta cttaagctaa gagacaaaag
     3241 ttctatacct ttgtttacta tatcatcttt atttgataat cttaaaaatc ccttaattat
     3301 ttgatatatc atttttgtat tttcttctct tttagcagca gtttctgctt catgctgtat
     3361 cttagacgta agagtgctag ttattatacc tactatcgag aatacaacca gtgttactat
     3421 atagtttgaa tctgctatag ataatgtata taatggtgct gtgaaaaaat aatttaaaag
     3481 aactatatta aatacagaac ttattatacc tgtactatac cctttagtcc acatagaaac
     3541 taaaactaca cccaacatat atattaacaa tatattttct cttataaatc ccatgctttg
     3601 tactgcatat gctaaaatac ttatcatcaa agtaataaat ataagtttta taaaatcaac
     3661 tttgctaatt ttaaatttag attttattga tgatttatcc tttttaggtt tgtatttaac
     3721 atctgaggca aatttatatg gaattatatg tatatctata taatcaactt catcaattaa
     3781 tttgtcaact atatcttttt taaatctttt actaaatctt ttattacttg aatgatttct
     3841 acctattaca atttttgtta catttctcag ttttgcaatt ctaagtattt gctctattat
     3901 attttcacca tgtaaaacaa ttatttctcc acctaatttc ttagctaatt ttaggttatt
     3961 ttgtagttgt ttttgttcat caaatggaag tcttgctgtg tttggagttt caacatataa
     4021 agctatccat tttgcaaatg tactgtctga tattctggat gcagttctaa taacttttgc
     4081 tgaagatgga gatggactta tacatgcaag aagtgtatct gatgtaggta ttacagttac
     4141 atcacccttt gatagtctgg tcatttgaac ttctttatta actctctctg ctgtttttct
     4201 aagtgcgatt tctctaagtg caaccaggtt atcttttata aaaaaattat tttttgctct
     4261 agtagcttgt tctttcttat atatcttacc ttcattgaac ctttcaagaa gtacatcagg
     4321 ttcaacatct ataagttcta actgtgtatt tgtatccaaa aacttatctg gtatagtctc
     4381 tttaactgat acgtttgtaa ttatctcaac aatgtcattt agactttcta aatgttgtac
     4441 atttacagta gtgtagacat ttatgcctgc taacagcaat tcttctatat cttgccatct
     4501 ttttgtatgc cttaacccag caacattaga atgtgcaaat tcatctacta atataacttc
     4561 tggttttctt gctaaagcag catctaaatc aaattctttt agttttatag atttatattc
     4621 tatctccttt acaggaagaa tttccaagcc atcaagtaaa gccaatgttt ctggtctagt
     4681 gtgtggttca atatatccaa ctactacatc tacacccact ttttttaaat tttgtgcaga
     4741 ttccaacata gagtaggttt ttcctactcc agctgcataa ccaaagaata tttttaattg
     4801 acctctactt agtttttctt cttcattttt aattttttct aatagaatat caggattagg
     4861 tctatccatt aaaaaacctc cttacaccat tcattactat tataatatca attccgatat
     4921 aaaattgtag ttaagatgta tttttaatga ttaatattat gtaaagattt aaatatgaat
     4981 ttaaaaaatt tagttgtaaa ataaaaatgc ctaacaatag gcatttttat cttgtgtata
     5041 atcattttag tttaagttaa gtatttccat aaactcatca cctgtaatag tttctttttc
     5101 taataaataa ttagcaagtt catgcaattt atcaatatta ttttctaaga tttcaattgc
     5161 atttttgtga caagccttta ctattttaaa tacctcttca tctattttag cagcagtgtc
     5221 ggaagaacaa gcaagagaag tatctcctcc taaatattga ttgcttacag tttcaagagc
     5281 gaccatatca aactcttcac tcataccaag gcgagtaacc atagctcttg caattttagt
     5341 agcttgttct atatcatttg atgctcctga tgtacaagtg ttaaagataa cctcttcagc
     5401 agcacgacca cctgtaaaag tagttatttt atcaattgct tcttctttag acataagtac
     5461 actctcatct tcagcaactt gcatagtata tcccaatgct cctgaagttc taggtattat
     5521 agtaatctta tggacaggtg cagaatgttt ttgcatagca gcaacaagtg catgacctat
     5581 ttcatgatat gctataattt tcttttcctt atctgaaatt acagctcctt tacgttgata
     5641 cccagcaatt acagtttcaa cagattcatc aaaatcctgt tgttctacta tatctctgcc
     5701 agacttaact gctaaaagtg ctgcttcgtt tacaatattt gcaagttctg cacctgatgc
     5761 tcctgatgta gaccttgcaa ttgcattata atctatatct gagcttacat ttacattttg
     5821 agcatgtacc tttaatatag cttcacgtcc tccaaggtct ggaagttcaa ctggtatacg
     5881 tctatcaaat cttcctggac gaagtaatgc tttgtcaaga ctgtcaggtc tatttgtagc
     5941 agcaagtatt actactccaa ttccaccatc aaaaccatcc atctcactca gcaactgatt
     6001 taaagtttgt tcacgttcat catttccacc taatccactt ccattatcac gctttttacc
     6061 aatagtatca atctcatcta taaacactat acatggagct ttttcttttg cttgtttaaa
     6121 taaatcacga atcctagaag ctcccattcc tacaaacatc tcaacaaact cagaaccaga
     6181 tattgagaaa aatggtacat ttgcttcacc tgctacagct tttgcaagga gtgtttttcc
     6241 tgtacctgga ggtcctacta aaagtgctcc tttaggcatt tgtgctccta tctttttata
     6301 cttttctgga ttgtgtaaga aatctactat ttcagttaaa gcttcttttg cctcatcttg
     6361 accagctaca tcattaaatc tttttccact ttgagcagat acatacattt tggcgttgct
     6421 ttttccaaat tgcatagcat taccacccat tcgcttttgc atagacttca tcattaagtt
     6481 accaaatagc ataagaatac ctataggtat tatccatgta agtaaaatac ttaaaagagg
     6541 tgaattttcc ttaggaataa ccttagtaaa ttctactttt gcatttttta gtctatctac
     6601 caattctggg tcatccattc taccagttat gtatatattc ttatccttgc tactagatgg
     6661 atttattgct attctattat tttctatctc aacagattta acctttcctg tatccaacat
     6721 tgtgagaaac tttccatagt caatctcaac cattttttta tccattaaac ttggaaatat
     6781 taaagcattt aaaaacatta ctactattat tataactgta taataataga gtgctgacct
     6841 tttttggggt ttatgttcat ccataatttt attctccttt aaattatttt tatgttattt
     6901 gcaatcaatg taagttaaaa gtttatttaa tttctctaaa ccttctatta tatcttccat
     6961 atcttgcaat ttttcactgg tcatattttc aaaataacta tcaaaaatac tacttgaaat
     7021 atgttcttgt atatccaatg atttatttgt aacttttact tttacaattc gttggtcttc
     7081 tatatctctt atgcgattta aaaagccatt tttttcaagc ctttgacaga ttactgatac
     7141 attactattt gtcatgttta agttcttccc taaatcgctt atttttttct ctccatgttc
     7201 acataactca gataaaatca aagcttgcac tgctgtaata ccaagtggct gataaaaatc
     7261 acttaataaa gttgtaaatt tcatatttat atctctagtc aactctagta gctctttctt
     7321 aaaactcatc tcatccatac aaacgctccc tacgaaaata ctttatttca aatttaggct
     7381 gaagtgcata ctataaataa atacttaaca ctataatgtt taatgtatta aatattatag
     7441 tattaagtat ttattgtcaa ttacaatatt gtaacttcaa ttgaaatttt gttatcttat
     7501 tgatatttaa ttttatacag gatattttta ataaaattta ataaaaaatc tatatatata
     7561 tattttacac tgatttctcc ctttttaatc tcttttaaaa gttaaaatac aaatttattg
     7621 atactataaa ggtttagtct cccattatcg caccatatat aaaattaaag ccacctctat
     7681 ctacatacac ctaaagtgat tttcttaaaa tataaaacta ctattataag gtcaaattaa
     7741 aaaaaaataa ggtataaagt atcattaaga ttaactttat acctataaat ttctatattt
     7801 ttttagttaa catttatata ctcttaaaat ttattgtatc aaatagtttt ttatccaatt
     7861 tctaaacaac aattctgaat gttttttcca ttttacagtt ggctcttctg aagggtcatc
     7921 attaatatag taattttgag gtatagatat tttatctcct ttatttatat ccctcttata
     7981 ttctttatca agagtgtatt tgtcatactc gctatgaccc attacgaaca catctcttga
     8041 atttgtaatt atatagggac cagcttcttt agaatgactc acaatgctca actctttatt
     8101 ctctttcaaa gcttcaatat ttacagtagt atgtcttgaa tgtggagcaa aaaattcatt
     8161 ttcaaatcca tctactattt tactatcttt atctacctta tgcttaaata ctccaaaaca
     8221 tttttgactt agtggaagtt tttttacatt gtagtatttg taaagacttg cttgagctgc
     8281 ccaacatata tataatgtag atttagtttt tctcttagaa taatccataa tttcttctag
     8341 ttcattccaa taatcaactt cttcaaactc catttgttca actggagctc ctgttattat
     8401 aaagccatca aaatactctc ctttggcttc atcaaatgtt ttataaaatt ctttaacata
     8461 ttcattacaa cttcttttac tttctcttgt tgaaatttta ataaattcca cagacacgtt
     8521 gaatcctgtt ttatctatcc ttcttagtaa atctaattct gtatctatct ttataggcat
     8581 tagatttagt atagctatct tagtgtctat gttttcttca tactttaatt cctttttaaa
     8641 atcttcttta taaattacat ctattccttc atctaataat ttattaataa caggtaatcc
     8701 ctttggtagt attaaagcca tatcttttca cttctctttc tcttagaata ttagaaattt
     8761 aaagcttgat ttaaatcagc tattaaatcc tctacatttt ctattccaac agataatctt
     8821 attagtgaag gcaatactcc agatgcaatt aattgttctt ctgttaattg tctatgagta
     8881 gttgaagctg gatgtataac acaagttctt acatctgaaa catgtgtaac caaagatgct
     8941 atctgtaatt tttccacaaa ttttttggca ttttctaacc cacctcttac tccaaatgat
     9001 aaaacaccac tacatcctct tgataaatac tttttggcat tctcataata cttgttatct
     9061 tcaagtcctg ggtaattaat ccaatctaca ttttcatgtt tttctaaaaa tctagctatt
     9121 ttaagtgcat tttcactatg tctctccatt ctaagatgta gtgtttcaac atttaaatta
     9181 gtaagatatg cattgaatgg gcttaaacaa tttccatagt ctctaagcaa ctgaactctt
     9241 gctttagttg catatgcggc atttccaaat ttttgagtat agcttatacc atgatatgtt
     9301 gggtctggct caactaattc tggaaatttt ccattatccc agttaaagtt tccaccatcc
     9361 actataattc ctccaacact tgaagcatgc ccatctaaat attttgtggt agaatgagta
     9421 actatatttg ctccatactt taaagggtta caaagcactg gagatgctaa tgaattatca
     9481 acaataaatg gaacattaat tctcttagct acatttgcta ttttttcaaa atctatgact
     9541 tcaagagttg gatttgcaag tgtttctgca aacacaacct tagtattttc ctttgcaagt
     9601 tctactatct catcttcact tgaatctaag tcaaacgata ttaaatctat accaaatttt
     9661 ttaagactag gtcctagtaa attgaatgtt cctccatata cttttgaaga acaaagtata
     9721 ctatctcctg atttacatat atttaaaact gccaacatat ttgcagattg ccctgatgat
     9781 acagctacag aagatactcc accctctagt aaacttattt tttcttcaaa agcttgaata
     9841 gtaggattgc ttattcttga atatatatgt ccatcaactt ttaaatcaaa taattcagca
     9901 agttggtcta tactgctata tttaaaagtt gtactttgat ataaaggtag tactcttggt
     9961 tctccattac ctggtttata atttccttgc acacatattg tttctttatt atacattctt
    10021 gtatccccct tagattttag taaaaaaaat taaactattt atcagatgag agttaaaata
    10081 aataaagcct gagtatataa ctcaggctct gtataagctt aattatactc atctctcagc
    10141 tttcgccgca ggatttagca ccaatgtata taaatatact ggttgccggg tttcataggg
    10201 ccagtccctc cacctctctg gataagattt atttaatttt tctatgtttt atatcttata
    10261 tatttttcct aattttgtca actatttttt taattttttt cttttaataa ttttcccaac
    10321 ctctacttga attacagaca ttatggctaa aacaatcgtt ataagccact ctaagttatt
    10381 taaaactgtt aagttaaaag catttctaaa aacaggtaca aacagtatta ctgctattaa
    10441 aaatgcagaa gttaaaaatg agattaaaag aaatggattt ttaccattat ttctattaaa
    10501 cattgaatct gtatttgaac actgactaaa tgctcgtaac atctgtgaaa atgctaatac
    10561 acaaaatgcc attgtttgac ctacagaatg attttctata ttaagaccaa ttttatatgc
    10621 taaaatagtc attattgcca caaatatacc ttgggtaaca actctttgta taagattttt
    10681 ttcaaacaaa gtggttgatt tcactggctg gtgcttcatt atatttctgc ttggtgggtc
    10741 aactccaaga gcaagggctg gaagcgtagc agttgcaagg tttacccata gaatatgaac
    10801 agcaagaagc ggagtatccc aatttaataa tattgctaca aacaacgtaa gtatctctgc
    10861 tatattacca acaagcaaaa attgtataac tttttgtatg tttctatata cacgtctacc
    10921 ttcttttatt gcataagcaa ttgttgtaaa actatcgtcc attaaaatca tgtcagaaga
    10981 atcttttgca acatctgtac cacttatacc catggctata ccaatatcag cagactttaa
    11041 ggcaggagag tcatttacac catccccagt cattgctggt acttctccaa ttctttttag
    11101 agattgtata attctaagtt tatcaaatgg ggatacacga gcaaataccg tagtattttt
    11161 aacagcttca tctagttcat catcactcaa attatcaagt tcatcaccag atatcacagt
    11221 attttcttca ctccatattc ctaactcatg agcgattgtt gtggcagtaa ctttatggtc
    11281 acctgttatc attatggttc ttatacctgc ttctttacaa gtacttactg catcaattac
    11341 ctcagttctt ggtgggtcaa tcataccaac catacctata aatgttaaat catattctat
    11401 attttcacta tcttcttttg gtatagatga aatctcacgc ttagcaaaac caagtacacg
    11461 caatgcatct ttagacatgt taagacataa ttcgtgtata ttctttttat ccacttcggt
    11521 aatgtttctt tcaccttttg aagttaatat tttagtacat aggtcaagaa tttcgtctac
    11581 tgctccttta gtataagcaa taatcttttc atctatatta tgaacagttg tcatacattt
    11641 tctatctgaa tcaaaaggct gttcaaatac acgtggatat tcttcttcaa gagcttcttg
    11701 atctattcca aattttttgg ccataaaaat taatgcgccc tctgttgggt cacctaatat
    11761 ttcatctgtt ttatctggat taatacatgc atcattacaa agtgcaccag cataaacaat
    11821 ctctttatac actaatggat gtttttcaga tgcagtatct attgatgtca cttttccaga
    11881 ttcaaaatct ccattgacag caatatgtgt aacagtcatt ttatttaagg ttaacgtacc
    11941 agttttatca caacatatta ctgtagctcc tcctaatgtt tcaacagcag gtaattttct
    12001 aattaaagca tttcttttag ccatacgctg tacgcctaaa gccattacaa tagtagctgt
    12061 tgcaggtaat ccctctggaa taattgatat ggcaagggaa attgcaatca taaacattgg
    12121 caagagaggt ctctgataaa atgcacctat tgcaaatata acaacgcata caataatacc
    12181 tatgacactt aatgtctttc caacagtatt aagtttacgt tttataggag tatcaaaatc
    12241 atcttgtgaa tctaaaagat gtgctatgtt acccacttct gtatccatgc cagttgctac
    12301 tacaacacct acacctctac catatgtgac tattgaagat gtatacgcca tattgctacg
    12361 gtctcctaat acacattctt taggtaagat tgtatctgca tctttttctg atggaacaga
    12421 ttctcctgtt aaagaagcct cttgaatttt taaacttgat gtttctatta aacgaaggtc
    12481 tgctggaacc atagctccat cttctaaaaa aacaatatct cctactacaa gattacttgc
    12541 tggaataaca ttttcttcac catcacgcat aacacgagct gtaggtgagt tcatattttt
    12601 taatgcttct aatgatgatt ctgctttttt ctcttgaaca attccaataa ctgcatttac
    12661 tgttacaatt attagtatta caaaagcttc tgtaaattcg ccaaatatca aagataatat
    12721 tgctgctcct attagtatca taaccatagc atctgtaatt tgactccaaa gcatttgtaa
    12781 aattgttcta ggtggttttt ttctaagttc atttaagcca ttctctttaa gccgtttttc
    12841 cgcctcggta tcggtgagcc cctcctctga tgaatctaga ctttctaaaa tttcatgcgt
    12901 tgctcttgaa tgccaaggca tctgtttgtg attttccatt ttggaagacc actcctttct
    12961 ttttttaatt taagaataat tatattttaa aatcaagttc aattcaatac acactaagta
    13021 tattttacct aaatttaaca atgccattta atatttaaca aaacttctac tcttttacaa
    13081 atatgtattc actattgtta gataagtttt ctttatactt ataaccttct aaattaaact
    13141 ttttcagttc ttcaatatct cttatcctat tttttacaat gtattttgcc atcatacctc
    13201 tagctttctt tgcttgtgta gattccactt tcaatatgcc atttttattt actttaaaag
    13261 tacatgtaat atatgtatca ctatctttaa taaatttttc tatggatttg ctgtattcat
    13321 ttgatgaaag attaacgata gctgtttttt ctttgattag ttctttatat atacgatttc
    13381 cccaatattc gtataaattc ttaaactcac ctactcgtaa ttttgtctgc atttctaatc
    13441 tatattcata tatactatca tatggtttta caacaccata caatccactt atgattcgaa
    13501 tacagctatt tgcaaactct tcatctttcc ttgtaaaatt ttctgcttct atatttttat
    13561 attgtattcc atcataagtt aagattgcag ggtttcctaa tctatcaaat ttaatgcttt
    13621 caaatctatt tttatttaat acagctaatt tttcattaat tttcatttta tttttaaaat
    13681 cttctatacc aaatgtcttt atattttcca ctatttcttt tgtgttatct ataaaacaag
    13741 gcaaactcaa atctctatca aatacttcta tattcttcat atttttagct ggtgataaca
    13801 ttataatcat aaattcttat tactcctgtt tttccaaata ttcaagaatc tcttgtgcat
    13861 ttgtgtctgg ttttgctttt tcaaagactt tttcaataat tccattttca tctataatat
    13921 atgtcgcacg tgcaactccc aatgcagttt ttccatacat ttttttctct ttccatacat
    13981 caaaagcttt tatagcaact aagtcagggt cagaaagtaa aataaaagga agttcatgct
    14041 tttctgcaaa tttttgatgt gattttatac tatctttact tatcccaata acttgtatat
    14101 cttccttttt aaatccatca tatgcatttc taaaagcaca agcttgacgt gtacatcctg
    14161 gtgtattatc tttaggataa aaatatacta ctactttttt acctttaaaa tctgacatac
    14221 ttaccttatt tccatcttta tcttctagtg taaattctgg tgcttttgtt cctatactca
    14281 acataaatat ctctcctttt gtttttatta ataattttat actatgctta tattattacc
    14341 ctataaatct atatataaat aaaatatttt attttttatg tataaagttt cataaatata
    14401 taattttcat gtatttatat gttaaaatag actgaaaata gttttattaa gatatctatg
    14461 cttaaaataa ggttaaatga tagattaatt aaaataagga tatctaaaaa taaaattttt
    14521 agttcatgag atgctctttt ttgtatgaaa tcaaatatat tttcatcatt tcaacaataa
    14581 aaagagactt atccctattt tgagaaagcc tcatttatta ttacttttat attatctata
    14641 taaatttaaa ttcctcaaca tcaaatagac ctaaatctgt aagtcttatt tgtggaacaa
    14701 ctggaagtgc cataaaagaa agtgtaataa aagggtctac atactctgga actcccattt
    14761 ttcttgctat ctccaacata ttatcagtaa tttcttgtac ttcttcagct gtaaaagtac
    14821 taattaatcc agctacttga agacttaggt gtgcaagaac ttttccattt gatacaatta
    14881 cataaccacc ttgtatttcc tttaagtaat ttatagctac tagaatatca tcatcattat
    14941 cccctgcaac tataatatta tgagaatcat gggccacagt agtagcaatt gctccatttt
    15001 ttatgccaaa tccttttagt gaagcaattg ctacatttcc agtcattcta tgtctctcaa
    15061 caacacaaag ttttgaatac tctttatttg gaacaaaata tccatctgca catggtaaag
    15121 attcatatac cttatttgtc aaaatttgat aaggtacaat ctccattaca taattttttt
    15181 cacttacttt taactgtatt ttttctttat taatatattt aaacttgact gtattaagca
    15241 attctttatc tttaatctca taattgtaat tagaaaacat ctcttcgttt accaaattac
    15301 catctttata tacactatcc acttccattt tgtctaaatc attcaataca acaatatctg
    15361 ccttataacc tgcacctatt gctccaattt tccttaaccc atatgctttt gcagaattat
    15421 aagttgcaat ttttatagcc ctaacaggtt ccatacctaa atcaatcgca cattttatat
    15481 tccatcttat gtgaccttgt ttttctatat catctaagtg cttatcatct gtgcaaaaca
    15541 taaattcttc aattggcaag ttatgcttta gcataccact tactatagac tttaaatttt
    15601 ttgctgcgct tccttctctt attaaaattt ttaatcctgc tctcaatttt tcatatactt
    15661 catcaaaagt tatacattca tggtcatttt ctattcctgc acatacatat gtctgaagtg
    15721 atttaccatt tatattaggt gcatgtccat ctacaacttt atttttaaaa agctctagtt
    15781 tatccaagat ttcattttca gaatttataa catcattaaa acacattacc tctcctaaac
    15841 ctaaaattct aggattattt acataactct ccatgtcttt tgcactaaat ttaccaactc
    15901 cattagtttc aaaacttgtt gcaggtacgg atgaaggcac cataatatat acattaagtg
    15961 gtatatcttt tgtcgattct aataaaaaat ctattgcact agctcctttt acattgacca
    16021 actcatgtgg gtcggctatg atagttgtcg tacctgattt taataatttt ttcgataatt
    16081 caattggcat taccattgta gattcaatat gcatatgtgc atcaataaag ccaggtgata
    16141 tatatttgtt gctacaatca atttctacat tgcccttgta atcgccaata ccaacgatag
    16201 tgtctcctac tattgctaca tctcctgtta ttaactcttc tgtaaataca tttatgattg
    16261 tcccattttt tagtacaatg tcagcttttt ctcttccact tgaaacatca cactgtcttt
    16321 ttaaattacc catttttaca actcctccta agttttttat atcaatatac tgactgtatt
    16381 acgtattata gtttaattta ttaatatata ttatatatga gttttgatag aaagtatcac
    16441 ttgaaatttg ttccataatt cctttaatta aatagagaga acatgaggaa taatattatc
    16501 tcccatgttc tcattaatat ttaagcaata atttaattca tattgaattt tttattttta
    16561 tatttaatct tcactattct tataaataaa tttatgtgct ttattatttt tttaatactt
    16621 ctttattcat aattatattt aacctaaaaa tgcataaact atcataccta caagtattgc
    16681 tggtatagta gtaataatca tagcccataa agaagatttt ttatccattg ctaaaagtgg
    16741 gaaaagtgca tctccatctt gagaaataga gttggctatc actgcaccaa gtggtatagt
    16801 tcccttcaaa taaaatgaca taagcacaat ttgtatccca caacctggaa taagtccaag
    16861 acctgcacct ataaatacac ttattacacc tgttgataat actaatttaa ctaagtattc
    16921 atctccacct aaaattaata ttatcaaatc gtaaataaga tatgcaataa aaatccatgt
    16981 aattacaaat gatatttctc caacagaatg gataagcatt tctcgcaaag aaatcttttt
    17041 attttcttcc tctttttgat tagaattttt aaataccttt ttaaaacacc acatataaat
    17101 tattgaaaat aatattccaa tcactgctat tacctcttcc aatgaatgta acttctcaat
    17161 tataggaaga ttaagtccag agtgagcaag tgacataaat ataaaaccga ttactataat
    17221 tcctatataa attttatatc cctttccatg tgttattata aatgcaagtc tatttgtatt
    17281 tccatgagat ttagctagag tgtctaaaat tatttcatca tgattatgtt gttctttatt
    17341 ttctttaggg ttatttttac ttatatcata tttatctgat tttctacggt tttttaaccc
    17401 taatttttct tctagtttaa agtaatccac taattgtcca gttattattc ctgtaattgt
    17461 actaactata gtgacaaaaa aatacatttt aatatttgat gaaataagta caaatgctgc
    17521 atctcccata ctagctatta aacttgcaat tattgctcca aagcttaatt ttccatttat
    17581 atacaatggc attattgcca aacttccgcc acaacctggt atagctccaa ttaaagcacc
    17641 tattaagggt tggaattttc tgttttttga tatgatatta gtaaaatttc cactagtttt
    17701 ataattaata tatccaaata acaaaataaa aaatccaatc atagaaccta catgtaaaaa
    17761 cgcctcttca gaagcggata tcaacatttc cattaattta cctcctagac taattttgca
    17821 acaattatat tatattctat tttctattga taatcaatat caatatttct tttgaatttc
    17881 taatattaaa aagtgcgcat cttatactct tagtttttca tcttaagata cacacttttt
    17941 gatattaggt tatctattta ccacatgtaa tatgaaatca aaggtggata ttatgataaa
    18001 ttataacaat gtccacctat ttttattacc tatcaaatta taaattttca aggaatgtta
    18061 caacttcttc tattatccaa ttaggtgtag atgcccctgc tgtaataccc aaatttttat
    18121 attttttaag cgtatttaca tccaattcat ctttagtctc tattgcaaaa gttggtacta
    18181 agtcttcaca aacctttact aacttttgag tatttgaact gtgtttacct cctataacta
    18241 tcatacaatc aacttcttta gctaactctt tagctgattc ttgtcttgtt tttgtagctg
    18301 aacaaattgt atcattaaat actatttctt ctaatttatc tgatagttta ttaactatac
    18361 ttatatacaa ctctggattt attgtagtct gtgcaacaag acaatatttt ttggagttat
    18421 caaattccat attttccatt tgttctaagg tctttattat tatagctgaa ttttcacacc
    18481 atccatttat acctataact tctggatgct tcatatctcc aataactatt atttcatagc
    18541 ctttattatg atactcactt actactgtat gtattttctt tacaaatgga catgtagtat
    18601 ccacaatttt taatttttta tctttggctt ccttatatat attttcagga actccatgag
    18661 accttattat catattttca tgatttggta ttgtatcaat ttcattcaca gtcttaagac
    18721 ctttttcttc atattttgct actgcttgct tgttatgaat aagaggacct aaagcatata
    18781 tgccagaatc atttttttca acttcatccc atgccatttt cattgctctt tttactccaa
    18841 aacaaaagcc agcatttttt gctattttta tgttcataac tttaccccct tattttgaat
    18901 atttaaagta attttgtttt atcacttcta aaatctcttg tgatatcctt tcatggtcat
    18961 cattactcaa tttttcctta aaatgttctt caaatgatat tggttctcca atatatacaa
    19021 ttactttact aaaaagttta tatttagata ttattgatac tggaacaact ttagatttac
    19081 tttttattgt aaatacagaa agacctgcct tagccttacc aaaagaatct ccttttattc
    19141 tagtaccctc tggaaatatt cctaatacat aaccatctct aactgacctt aaaatagttt
    19201 ttattgtaga tacatctggt ttttctctat ttataggcat aacatgcaat ttttttaata
    19261 taaatcccaa tggctttact ttaaacaatt ctttctttgc aattgcagct atttccctat
    19321 tttctatggc agcagccaag aatatagggt caagattcga tttatgattt gatgctatta
    19381 ctatatttcc tctatctgga atattctctg cacctataac ttcatattta aaaaatattt
    19441 tagaaaaacc cttaaaaata tttattacaa atctataaaa attcacgttt atctcccttc
    19501 aacactacat ttattttcat tatgatgcat aaaaaactaa agatttattt tagatacaac
    19561 ttcttctaca acttcttcta tagtttttcc tgtagtatct atttctagtg catcattagc
    19621 ttttaccaat ggtgcaaatt ctctatttga atcaatttca tctcgtctta ttatatcctc
    19681 aattacttct tctaaagtag tattatatcc tttattacaa agttctttat agcgtctatt
    19741 tcctctttct tctggagtag ctactaaaaa gaatttgtaa tctgcatctg ggaaaacata
    19801 tgaacctata tctctaccat ctagtattac tgaatttttc attcctattt ttcgttgaac
    19861 ttcaaccatc aactgtctta cttcttttat ttttgctaca ttagaaacat tattgcttac
    19921 ttctattgtt cttatttctt catttataac ttctttgtcc aaatatatat tgttatcttt
    19981 aaaatctata tccgaatttt cagctatttg aataacttct ttttcattat tgacatcaat
    20041 accacttttt aaacacttat aagtaactgc tctgtacata gcacctgtat ctatataatt
    20101 tatatttagc ttttttgcta ctatcttggc tattgtactt tttccagctc ctgctggacc
    20161 atctacagct ataactaaat tacccatata tacacctcta tttattcata tttaattatt
    20221 aaaaaattat atttatacac ttatacccgc aagatatcca gttgaaaacg ctatttgtaa
    20281 attatatcca ccagtttctg catctatatc aattacttca cctgtaaaaa ataagttctt
    20341 aactaattta gattccatag tagaagaatt aatttcttta actgaaatac caccagaagt
    20401 aaccatacca gtgtttatac ctgtagtccc attacaagtt agcttcatat tttttatata
    20461 ctcaataatt ctaatttcat ctgccttaga caaatcactt gcctttttat caactaaaga
    20521 taatagagaa aaaatctctt ttaagaaatt ttgaggtagt atttgcttaa gattatttag
    20581 aacattttta tttggaaatg ctctgattat actagagatt tcatcagtac ttaaatttgg
    20641 taagaaatct atatttaatt ctaccttttc cttttcaata atcttgttaa tataagaaga
    20701 cattataaga acacaaggtc ctgttatccc aaaatgggca aatagcatgt ctccagattt
    20761 tgatattttc cttttcttaa tcttacaaga tatttccaca ttcttcatgg atattccttg
    20821 taaatcttta atccattttt cctctattgt aagtggaact aaagcaggat atagtttgtt
    20881 taatgtatgt ccatgctttt taagaatctt atacattgaa ccatctgaac ccgtctttga
    20941 ataactcact cctccagttg atataataac cttgtctcct agtattttat ctccattatc
    21001 taaaataatc ccttttatta gatactttga cttgtcctta ttagaatcat cttttagagc
    21061 aatttcttca gttataatat ctataacttt cttattatac attatcttta cattgtgatt
    21121 gagtaaatct ttatttaata cttctattac ttctatagat ttatcatttt tagtgtatac
    21181 tttgtggtca ttttcttctt caatcttata ttccaaattt ctactttcaa aatacgaaat
    21241 aaggtcttta tttgtaaatg tatagaaact actatagaga aattttttat tactgacaac
    21301 tttatcaaaa aaatcttcta tctctctatt attggtaaaa ttgcatctac cgccaccagt
    21361 tgccctaagt tttcttccta attctccatt tctctcaact aatattactt cattattgtt
    21421 ttttgaagct gacaaagctg ccatcattcc agaagctcct ccaccaacta ctataacttt
    21481 actcataaat ttctccttat aattgattcc tactccagtg cagaattatt tatctttaca
    21541 tgatttttta tatgacttgt atcattcatt tttactataa caggtccata atctttaaat
    21601 tctactatat ttaaagcagt attatcttgc ttaattctat atatattttc catacctgat
    21661 tctaaaaatg acaacaacat aaccctcaat gtaattgaat gtgtaacaag aattatattt
    21721 ttattatcat atttttcatt tagttccttt ataaaagcat ctactctttc ctttataatc
    21781 ttaagagttt cgccttctgg tatatttact aagtgtggtt catttctcca tgttgcatat
    21841 atatctgaat aatctttttg aatttctttt gttaaaaggc cttcccaaac tccgaatccc
    21901 atttctctaa gtgcctcagt tttttgaact tcaatattga gcttatctcc taaaatttga
    21961 gctgtttgca ttgctctccc aaggtcgcta ctaaaaatat aatcaattga atacttgcca
    22021 atatcttcag aaagttcttt tgcctgttct ataccttgag gcgtaaggtc agaattacca
    22081 tgaccttgtg tttttcccaa tatattccaa tccgtttgtc catgtcttac tatataaaaa
    22141 gtatttccca tatgtcctcc taatatatgc ctatttttta ttaaatataa attatcactt
    22201 ctcaaaactt aattatatca tataatactt tactttgttt ctattttata atctaatcta
    22261 acatattatt tacaataaat ctcatttgta tttctgcaaa ttattgtact gttaaagaat
    22321 ttttctaaat ttataaacat atttctctta tattatttat atacttcttc aaatctgtat
    22381 tcttgaattg aattgacata cttatctgta tctacttttt ctaaaaacat atccaaaggt
    22441 cttgcaaata ttcctgtatc atcatataaa gtcttgtata aaactaaatc attagtatct
    22501 tcttcttttt tatgataaaa gtttccatct aaatccctat aaacatagat atcttcttct
    22561 ctttcagtat gtctaataac taatttatat ttatttctgt tttggattaa attttcttta
    22621 ccatatattt cacatatgtt ttctatatca tcaatagcct tgctaagacc cattgtagca
    22681 taatatttcc ctttgaaatg tttatagata caaggatatt ttaactttct ttcactgata
    22741 cctaagttat tctttaattc aagcatttta ttataatctc ccatatctcg tccctttctt
    22801 ttaaaaagta ttttattctg aaataagtta cttgttttat attttatcat aatttaaaat
    22861 ttattaataa tacaatcaat aaataaagag gctgtataaa atacagcctc tcaatattaa
    22921 tcttcataat catataattt ttctaatttc ttttttaatt tcttttttct ttctgaacct
    22981 aaaattctta taaaattatt attcacaaaa atacaattat tgctgagata aaattcacac
    23041 ttgtctagat ttacaatata actattactg catttgaaaa aattttcttc tttcaatagt
    23101 tttcttatac aaggagcatt ttctccaact tcataattac cactttcagt ataaatcttt
    23161 acttcatact cttgcttttc tatgtacaaa acttctttac tattaattga aaaacgttta
    23221 tcctcgtatt caacattaat aaaattttcc tctttcattt ccatccctcc aagtactttt
    23281 attaatttaa ttttagtgtt ttttatcaat ataatctatc ggaaatatat gtaagactga
    23341 tttacttaag atatttaaat aattattatt aatattaatg tataagttta ttttttaaca
    23401 ttaattttta aagttaaaaa
//
LOCUS       DAEMHW010000046        23203 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEMHW010000046 DAEMHW010000000
VERSION     DAEMHW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23203
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.046"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            101..1387
                     /locus_tag="KPK67_001549"
     CDS             101..1387
                     /locus_tag="KPK67_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG2458537.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1499..1972
                     /locus_tag="KPK67_001550"
     CDS             1499..1972
                     /locus_tag="KPK67_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1631..1633,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBG2458538.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            2004..3314
                     /gene="grdB"
                     /locus_tag="KPK67_001551"
     CDS             2004..3314
                     /gene="grdB"
                     /locus_tag="KPK67_001551"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3051..3053,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG2458539.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3570..5102
                     /locus_tag="KPK67_001552"
     CDS             3570..5102
                     /locus_tag="KPK67_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBG2458540.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVRPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5113..6258
                     /locus_tag="KPK67_001553"
     CDS             5113..6258
                     /locus_tag="KPK67_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBG2458541.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGAENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6568..7647
                     /locus_tag="KPK67_001554"
     CDS             6568..7647
                     /locus_tag="KPK67_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2458542.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEVQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7760..8359
                     /locus_tag="KPK67_001555"
     CDS             7760..8359
                     /locus_tag="KPK67_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458543.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SSQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(8546..9418)
                     /locus_tag="KPK67_001556"
     CDS             complement(8546..9418)
                     /locus_tag="KPK67_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2458544.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            9835..11238
                     /locus_tag="KPK67_001557"
     CDS             9835..11238
                     /locus_tag="KPK67_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458545.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            11265..12809
                     /locus_tag="KPK67_001558"
     CDS             11265..12809
                     /locus_tag="KPK67_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG2458546.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDSMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            12840..14231
                     /locus_tag="KPK67_001559"
     CDS             12840..14231
                     /locus_tag="KPK67_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBG2458547.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            14424..15920
                     /gene="abfD"
                     /locus_tag="KPK67_001560"
     CDS             14424..15920
                     /gene="abfD"
                     /locus_tag="KPK67_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBG2458548.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            16055..16330
                     /locus_tag="KPK67_001561"
     CDS             16055..16330
                     /locus_tag="KPK67_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458549.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            16354..17661
                     /locus_tag="KPK67_001562"
     CDS             16354..17661
                     /locus_tag="KPK67_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG2458550.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            17681..18799
                     /locus_tag="KPK67_001563"
     CDS             17681..18799
                     /locus_tag="KPK67_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG2458551.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVLPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLGDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            19086..20006
                     /locus_tag="KPK67_001564"
     CDS             19086..20006
                     /locus_tag="KPK67_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBG2458552.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            20121..21278
                     /locus_tag="KPK67_001565"
     CDS             20121..21278
                     /locus_tag="KPK67_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG2458553.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKEITMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(21363..22862)
                     /locus_tag="KPK67_001566"
     CDS             complement(21363..22862)
                     /locus_tag="KPK67_001566"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBG2458554.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         8945 a         2739 c         4378 g         7141 t
ORIGIN      
        1 aataatatat ctttaaaaaa ataagataaa ttgtaaaatg atataatttt gagaaaaact
       61 caaatgcata tacacaaatt aaataacagg aggtgcaagt atgcgtcttg aattagggaa
      121 aatctttata aaagatattc aatttggtga tgtaacagaa gtaaaagatg gagtattatt
      181 catcaataag caagagatgc tacaagaaat tggtggagat gagcatatta aatctataga
      241 tatagaatta gctcgtccag gtgaaagcgt aagaataaca cctgtaaaag atgttattga
      301 gccaagagta aaagttgaag gtaatggggg aatcttccct ggtatcatgt ctaaagttga
      361 cactgtagga gaaggaaaaa ctcatgcttt aaaaggtgta gctgttgtaa ctactggaaa
      421 aatagtaggt ttccaagaag gtataataga catgactgga gaaggagcta agtatactcc
      481 attttctaaa ttaaataatg tagtagtaat agctgaacct attgatggtt taaaacaata
      541 cgctcatgaa aaagctgtta gaatgatagg attcaaagca gcaatgtact taggagaagt
      601 agcaagaaat cttactccag atgaagtttc agtatatgaa actaaacctc ttcttgaatc
      661 aataaaagaa tatccagagc ttccaaaagt aggatatgtt tacatgcttc agactcaagg
      721 tttattacat gatacttatg tatatggagt agatgcaaaa caaatagtac caactttatt
      781 atatccaaca gaattaatgg acggagcaat tgtaagtggt aactgtgttt ctgcatgtga
      841 caaaaaccca agttatgttc atataaacaa tggggttgtt gaagacttat atgcaagaca
      901 tggtaaagat ataaactttg taggtgtaat tataactaat gaaaatgttt acctagcaga
      961 taaagaaaga tcttcaaact ggacttctaa attatgtaaa tatttaggat tagatgcagt
     1021 tatagtttca caagaaggtt ttggaaatcc tgatactgac cttataatga actgtaagaa
     1081 gatagaaatg caaggtgtta aaacagttat agttacagat gaatatgctg gacgtgatgg
     1141 aggttctcaa tcacttgcag atgcagatgt aagagctgat gctgttgtaa caggtggaaa
     1201 tgccaaccaa gttgtagttc ttcctaagtt agataaagtt attggacatt tagaagtagt
     1261 agatgtaata gctggtggtt ctgatggaag tttaagagct gatggaacta ttgaagttga
     1321 aatccaagct ataacaggtg caactaatga aacaggattt ggtcatttaa ctgctaaagg
     1381 atattaagaa gtattttatt aaacgattga gtaatgttaa taaaacatat tatagataat
     1441 tataataagt gttatagata tataaaaata tattagaaat ataataagga ggattattat
     1501 gagtttactt agtaataaaa aggttcttat aataggtgac cgtgatggta taccaggacc
     1561 tgcgatagaa gaatgtgtaa aaacagtaga aggagcagag gttgttttct catctacaga
     1621 atgctttgtc tgaacagctg ctggggctat ggacttagaa aatcaaaaca gagttaaaga
     1681 tgctgctgat aaatttggag ctgaaaatgt tgtgatttta ctaggtgctg ctgaagccga
     1741 agctgcaggt cttgcagccg aaacagtaac tgctggagat ccaactttcg ctggaccact
     1801 tgctggagtt gccttaggat taagtgttta ccacgttgtt gaggaaccaa taaaatcatt
     1861 atttgatgaa agtgtatatg aagaccaaat aagtatgatg gaaatggttt tagaagttga
     1921 agaaatagaa gaagaaatgt ctggtataag agaagaattt tgtaaatttt aatgcctaaa
     1981 agcaaaaggg gtgaaactaa gagatgggaa aactaaaagc agtacattat attaatcagt
     2041 tctttgctgg tataggtgga gaagaaaaag ctgatactaa gccacatgtg gcagaaactt
     2101 tacctccaat aagtttacaa cttgataaat tattaggtga agatattgaa atagttggaa
     2161 cagtagtttg tggagatagc tatttcaatg agaatataga tagtgcaagt gaagaagttt
     2221 tatcaatggt aaaaggtttt gaaccacaac ttttcatagc tggtcctgca tttaatgctg
     2281 gtagatatgg ggttgctgct ggaacaataa ctaaagttgt aaaagatgct ttgaatatac
     2341 cagctttaac aggtatgtat gttgaaaatc caggagcaga tatgttcaaa aaggatgttt
     2401 atgtagtaga aacttctgat tctgcagcag gtatgagaaa ggcacttcct aagatagcta
     2461 aacttgcagt aaaacttgct aatggagaag aaattggaac tcctaaagat gaaggataca
     2521 tagctagagg aataagagtt aactacttcc atgaagacag aggttctaag agagctgttg
     2581 acatgttagt taagaaaata aaaggtgaac catttgaaac tgaataccca atgccaaact
     2641 ttgatagagt tgacccaagt aaagctgtaa aagatttatc aaaatgtaaa atagctttag
     2701 ttacttctgg tggtatagtt ccaaaaggaa atccagatag aatagagtct tcttcagctt
     2761 ctaaatatgg aacttattct atagcaggag ttatggattt aacagaagag acttatgaaa
     2821 ctgcacatgg tggatatgac ccagtgtatg caaatttaga tgcagataga gtattacctg
     2881 ttgatgttct tagagattta gaaaaagaag gtgtaattgg aaaattacat gagactttct
     2941 atacaactgt aggtaatggt acatctgttg caaactctaa aaaatatgct tctgaaattg
     3001 gagcagcatt agtggctgat ggtgtagatg cagtaatatt aacttctaca tgaggtactt
     3061 gtactcgttg cggtgcaacg atggtaaaag aaatagaaaa aactggactt ccagtagttc
     3121 atatgtgtac agtagtacct atttcattaa cagtaggggc taatagaata gttccaacta
     3181 tagcaatacc tcatccactt ggaaatccag cgcttgaccc tacagaagaa aaagccctaa
     3241 gaagaggcct tgtagaaaaa gcattaaatg ctttaactac agaagtagat ggtcaaacag
     3301 tatttgaaaa ataaaaataa cacacacata tattatatag ataaaaatat aaacacacac
     3361 taaaaataaa aaataactat acacaaaaaa ataatataga tttcatgaat ggataaattg
     3421 gagttattca ttcatgaaat cttaaaaaat gccaaaatac ataaacaata atagaatcaa
     3481 atatacaata aaatacaatg attagagctt ttaataagtt taataagatt aaaaaataag
     3541 tgttcataaa atttatggag gtgcacaaaa tgacttatcc agttttaaaa ggggcaggct
     3601 atgtacttat tcatactcca gatatgatag tgcaaaatgg aagtacttgt acagttgaaa
     3661 gagcaacaaa tccagattca gaatttttaa aagaagtaag taatcatata agaagttatg
     3721 aagatgtagt taactacatg ccaaatcaag tttatatagg aaatagaaga ccagaagaat
     3781 taagagattt accaatgcca tggtgtgaac aaaagattga gggaactaga aatggtaagt
     3841 ttggagaaat aatgcctcaa gatgagttca tagctttaat gcaaataagt gatgcatttg
     3901 atttagtaaa attatcacaa gaatttattg atgaagtaag gccaaaaatc gaaaataact
     3961 accctgaaat tgcaccattt gtaggtaaat taaagggtga tgatatagaa gaaggtaaag
     4021 aattagtagc aacacacata gctgaaggat tataccatga tggaaaattt gttggttatg
     4081 taaaaagagc acatgatgta gatgttaact taaatgccca tacaatgttt gaaaatctag
     4141 tagtaaaagc atctggagtt ttatctgcta ttcaaatgct aagacatagc aaaatagacc
     4201 ctgctgaaat agattatgtt attgagtgtt cagaagaagc ttgtggagat ataaaccaaa
     4261 gaggtggagg aaactttgct aaatcaatag ctgagatagc aggacttcaa aatgcaactg
     4321 gttctgacac aagaggattt tgtgcagcac caactcatgc attaattcaa gcagcagctt
     4381 tagtaaaagc tggtatacat aaaaatgtaa tggtagtagc aggtggagca agtgctaaac
     4441 ttggaatgaa tgctaaagac catgttaaaa aaggtcttcc tgtacttgaa gatgttgttg
     4501 gaggatttgc agtacttgta tctgaaaatg atggtgtaaa tccagttata agaactgatt
     4561 taacaggtaa gcatactgtt ggaactggtt cttcaccaca agcagttatg acagcactta
     4621 taacttcagg tcttgataga gctaatttaa aaataacaga tgttgatgtg tattcagttg
     4681 aaatgcaaaa cccagatata actaagccag caggagcagg agatgttcca gaagcaaact
     4741 acaaaatgat aggggcactt gctgttaaac gtggagactt agaaaagaaa gaattgaaag
     4801 attttgtttc taacaaagga ttaccaggat gggcaccaac tcaaggacat ataccatcag
     4861 gagctcctta tataggattt ttaatagatg atttaactac aggaaataga aatagagcta
     4921 tgatagttgg aaaaggtagt ttattcttag gaagaatgac taacttattt gatggagtat
     4981 cttttatagc tgaaagaaat acaggtgtta ctgaagagac ttcaggaata tctaaggatg
     5041 aaattaagaa gataatagct gaatctatga aaaaactagc attagatatg ttagaagaat
     5101 aggggtgagg atatgtcaaa aaaagtaatc gcagatgtat tcttagaagt agctaatgca
     5161 atagaaagcg gcgaatttgg caaaaaagta aaaattggtg ttactacgct tggaagtgaa
     5221 catggtgcag aaaacatggt taatggtgca caattagcaa aatctaattt atttgatatt
     5281 gtgcttattg gaccaaaagt ggaaactgac cttgaagtag ttgaagttaa tgatgaaaaa
     5341 gaaatgcatg caaaaatgga agaattatta gattcaggat atattgatgc ttgtgttact
     5401 atgcattata attttccaat aggtgtatct acagtaggta gagtaataac tccagcaaaa
     5461 ggaaaagaaa tgatacttgc aacaacaaca ggaacaagtg ctactaatag aatagaggca
     5521 atggtaagaa atgccattta tggtattgct acagctaaat caatgggtaa caaatgccca
     5581 aaagttggaa tacttaatgt tgatggagca agacaagttg agaagtgctt aaaagaatta
     5641 aaagataatg gatatgacat ggagtttgcg gattcaatta gagcagatgg tggatgtgta
     5701 atgagaggta atgatttact tgtaggtgca cctgatgtta tggttactga tacattatct
     5761 ggaaatatat ttatgaaagt attttcttca tatactacag gtggagatta tgaagctcaa
     5821 ggctttggat acggacctgg agttggagag gactatgata gaaaggtact tatagtatca
     5881 agagcatcag gttctccggt agtagcaaat gctttaaaat acgcttatga tgttgtaaag
     5941 ggagatataa gtaatgttgc aagaaatgaa tttgcaaagg ttaaaaaagc aaaatttgat
     6001 gacattatat cctctcttac taaaaaagaa gttaaagctg aaaaagtaga agttaaaatg
     6061 ccagataaag aaattgttac tcgtcaaata gctggtgttg atataatgga tttagaagat
     6121 gcagtatctg agttatggaa aaacggaatc tatgctgaaa gtggtatggg ttgtacaggt
     6181 ccaatagttt tagtaaatga agctaaagga gacttagcag ttgaaacttt agttaaagct
     6241 ggatatactg caaaataagt acatttttta tatgtaaata agaacatata gttataattt
     6301 tattgattat aattatatgt tctttttatt ttatatcttg tcaatattta tatattaaca
     6361 caatccattt atttgattct ctagctaatt tattatattt attcagttaa aattaagctt
     6421 tctaactaaa taaaaactaa tgaagtatta aaatgaaatt gataaaatat gaaaaaattt
     6481 taggaataaa gtatctgtta gataaaaata agattaatta gtgatataat ataattaaga
     6541 taaaatttat tatttggagg taaaatcatg agcagagtaa aaaatgtagt agaattgttg
     6601 gaaactaaag gtgttgatgc actatatcta acaaagaaaa ctaatgtaaa ctatataagt
     6661 ggttttcctg atgaagaagc atatgcagta atttgcaaag atggaaactt tttagttact
     6721 gatagtagat atatggagct tgcagaaaag gtttgcaaag attttgaaat tataaattgg
     6781 cataattttg atagaagtgt ggcaaaggca gttaaaagtg tatgtgataa agtgggtata
     6841 aagaaacttg ggtttgaaag aactaatata gtgtttgata aatatgaaga acttaaaaat
     6901 ctgattgaga aggataatgg agaattaata cctacagaaa atattgtaga gactttgaga
     6961 tatgtaaaag acaaagatga aatcaaaaat acaagaaaag cttgtgaaat agcggataag
     7021 gcattagaag aattgattcc acatataaaa gctggagtta gcgaaattga actggctaca
     7081 aagttagagt actttatgaa aatgaacgga gcacaaaata tagggtttga aactatactg
     7141 atatcaggag caaagacttc attgcttcat ggtaagccta gtgataagat tatagaaaaa
     7201 ggtgattttg tacttataga ctatggtgct atgtataatg gctatatatc tgatacaact
     7261 agaactttta tagttggagg agcttctgaa aagcaattag aaatatataa tttagttaaa
     7321 gaagttcaaa atgtaggggt tgagaacatg aaagctggtg tacatgcgac aattccagat
     7381 gctgagataa gaaaagttgt caaaaaatat gaagactatt actatcaagg tataggacat
     7441 ggagtcggta gagatgttca tgaagaacct ttcattggta attatggtga taaaatcata
     7501 gaagagggat gtataataac aatggaacca ggtatttatt tccctggatg gggtggagtt
     7561 agaatagaag atactgtttt gattactaaa aatggtccag aaagacttac taaattccct
     7621 aaagatttga tgattttaga caaataattt aatttatata tattttttta cataaaatat
     7681 aacataatct cactattaag ctctatttac atattatagt aaagagggct taattttatt
     7741 gtgagaggtg atagtatata tgtataaaaa acataaattt ataaaaaata taaaaaaagc
     7801 ctggtataaa atcaaattaa ttgatagatg ccttataatt gtaatggcaa tattaatgtt
     7861 tcaatctata tataatttat ttgttaatga agtaaattca caagatacta caactattga
     7921 tgtagtagta agaaccactt cagcagctat atttgggtat tttttaagtg ctaattttat
     7981 aaagagacct ttaaggaagt atacaaatac tgatgtatct tcaaatcttt ttatagataa
     8041 taatttatcc ccaaaaggtc aagagtctag tcaaaataat ataatgaatg tcaaaaatac
     8101 cattggattt acttcagaag ctgataatta tgaaaagaaa atacctatta ataataatga
     8161 aggttttgaa gaaggagaaa ctagtgaatt gcaaattatt atagctactg taatttgtat
     8221 agtagcatta acagtacttt ttgttgtaag aaattttaca actacaacat cagcttcttt
     8281 aggtactatt tcacagatga gagattttgt ctcaggatgc gtgggttttt tgctaggttg
     8341 tccaagtaaa cctgattaaa aaagtattat caataaaagg tattttaaaa agtatcagta
     8401 taataatatt tagatttaaa tgtatacaat tataattcta taacattcta tattgtattt
     8461 ttatactgat actctaaact atactattta ttaaaatatt ttaacattgt atttaaacaa
     8521 aatattatat ctgtataata taaatttagt ttatatgaat agatgccata tactcaataa
     8581 aaagtttcat actatgagat atgtattgat ttcttctcca aagaacccca atatttgcag
     8641 taattgcacg ttctaatggt attgaacaga gattttctgt attttgaaat atttctttat
     8701 acaatattgt tgaagccaag tcattttcta gtaaataacg tatggtagaa agttgatttg
     8761 agtgtaaaat tatatttggc ttagcttttt caagtctttt tgtaataatc ttatagtgat
     8821 aagaaccttc agataagatt acaaaaggta cattttttaa catataattc gatataattg
     8881 gatgttttgc caattcattt ttgggatttg taactaagta taattctgtt ttatagattg
     8941 tctttgaatc tatatcatca taaatatcac tgtcaattat accaatacaa aaatcaagtg
     9001 tagattcttc aatcatttta gctccaacaa atgttggaac ttcaaaaatt ttaagctcta
     9061 tatctggata aattacatta aatctaggaa gtatttttga aagcaaaaac gtacctaaaa
     9121 ctgtaggaat acctattttt agagaagctt ttctcttttt tcctaaatca agtgcgtcac
     9181 attgaaaatc ctcaaattgt tttataaaat gttttgactt gtctaaaaaa atttttcctt
     9241 caggcgttaa ctctatttta ttacctattc tgtaaaaaag ttcaaagcca agttcttttt
     9301 ctaattcttt aattgctaat gttatggatg gttgagaaat atgaatactt tccgcagcct
     9361 tagtcatgtt tttatattta cagacagttt gaaaatagta tagatagttt atattcattt
     9421 ggtattagtt cctttcattt acatattagt gacaagtata taattaacaa tagcataagt
     9481 tttgtatatt gttgtaaaaa aatagaatag ttttaaacta tcatagcata agtttttgat
     9541 attttcaatc tattaagaat agtgctatta ttaaattaac aaaagagaat agctggtatg
     9601 gtttagagta aatcaatcta tattacagtt atatcaacga atatcaaaaa attatctatt
     9661 agaaaataaa aattattttt acaattaaat tgttaattaa atcataaaga aaaaagtgat
     9721 attaacaaga aaaaattttt ttaatcagaa atgttaattt aataacaaaa tagagtattg
     9781 gggagaaagg agaaatatat tttttaataa taattgttaa tttaataacg aaaaatgaaa
     9841 gggagtgatt tagtgaagtt tgaattgatg gattttttaa tgaatccttt tgtccttatg
     9901 tttgctgcag taattacagg aattttattt ggaaaaatta aatttgggaa gtttaatttt
     9961 ggggtatcag gagcattatt tacaggatta tttattggat ggcttgcata tagcttgggg
    10021 aatctaataa tagcaaaagg ggaaacagca gcaggatata aggcagcaac tgtaatgatg
    10081 gggaatggaa ttatttcttc tgacttcttt gatttctttt taataatatt tgttgcagca
    10141 gttggtctat tggcagcaaa agatatgaaa gctgtattaa aaaagtatgg agcaagattt
    10201 gtaattttag gagttcttat aacttttata ggtggctcta tgacttatgc aatgacttta
    10261 ctatcaagtg ataaaggaag tagtgcttat gaagtttctg gcgtttacac aggagcatta
    10321 acaagttcgc ctggattagc tgctgcttta gaaacagctg gtaaacatgc agaagatgta
    10381 tcaaaagaat ttgaaaaagc ttcaataaaa gataaaaaag aaattttaaa agtagtcgac
    10441 ccagaaggca aattggatgt gaatacaact acatcactga ctcaagagca aatagataag
    10501 tatattgcat atgctgaagc tggtgttggt ataggtcatg cagtcgcata tccttttgga
    10561 gttttaatag taatcttagg tgtaaatttc cttccaaaat tatttaggat ggacctaaaa
    10621 gaagaaagaa gaaaatatga gaaggaaatg aaggaagcaa gagatagtgt ttctggtaaa
    10681 acgataccag aagttccatt taatataata acatttttct taacatgttt agctggatac
    10741 ttagttggag gaatacatgt atttatgggg ccactagggt acttcacact aggtgcaaca
    10801 ggaggttcac taattgtatc tctagttctt ggatatatag gtaagatagg ggtagtaaat
    10861 ttcagaatgg aagaaaaggt tttaaatatt ttaaaacaaa tagggctagt atttttctta
    10921 gcaatagttg gattaagata tggtggaaaa gtagttgatt caattatgac atctggtatg
    10981 cacctagcct tagtagcaat agcagtaggt gttacagcta tgatgatagg attcttagtt
    11041 ggcaaatacg tatttaaatt aaattggatt ttattatctg gagctgtctg tggtggtatg
    11101 acatcaacac caggtcttgg agctgctgtt gatgctcttg atagtgatga cccagcagca
    11161 ggttatggtg ctacgtatcc atttgcatta ttaactaaag ttatattggt tatagtttta
    11221 cacaaacttc caatgtaaat aagattttat atggggggat atttatgaat aatgaaggag
    11281 catttgaaga attaagtgga agattaagaa ataaagaatt gtgtgattta ataatgaatg
    11341 cagatgaagc tgctaaaatt atagaggata gcatggttat tggagttagt ggatttacac
    11401 cttcaggcta tccaaaagca gtaccattag cagtttcaga aagagctaaa agtggagaaa
    11461 agataaaatt aaccgtatat tcaggagcat ctctaggtcc agaagttgat ggagcatggt
    11521 cagaagcagg tataattgaa agaagacttc cttatcaaac taattcaata cttagaaata
    11581 atattaataa aggtgttgtt gattatatag atatgcacct aagtcattct actcaatttt
    11641 taaattatgg aactatacct aaagtagatg tagctatagt agaagcactt gcaataactg
    11701 aagaagggaa tattatacca actagtggga taggtaattc tccatctttt ataaaaagtg
    11761 cagataaagt aatagtagag ataaatcttg caaaaccaat ggaaatggaa ggtatggcag
    11821 atatatatat tacggaaaat ccaccaaata gaaaaccaat agaaataaat catcctaagg
    11881 atagaattgg aacaacatat ataccttgtg gtttggataa aattgcagga gtagtaatta
    11941 ccaatatgca agataagacc cgtccattgg gagttgtaga tgaagcttcg aaaaaaatat
    12001 caaataatat tattgctttt ttaagagaag aagtaaaggc aggcagatta tcaaagaatt
    12061 tacttcctct tcaatctggt gtaggaagtg tagcaaatgc agtattatat ggattatgtg
    12121 aatctgaatt tgaaaattta acttgttata cagaggttgt acaggactct atgttggatt
    12181 taataagaat gggtaaagtt acaatggcat ctactacatc tgtaagtcca tctccagaag
    12241 gattaataaa atttgaaaaa gatattgatt tttttaagga taagattata ttaagacctc
    12301 aagaaattag taacaatcca gaaatagcca gaagaattgg tgtaattgca atgaacactg
    12361 caattgaagt tgatatttat ggaaatgtaa attctactca cataatgggg tcaaagatga
    12421 tgaatggaat tggaggttct ggtgattttg ctagaaatgg agcaataact atatttagta
    12481 ctgagtcgat tgcaaaaaat ggtgatatat catcaatagt acctatggta tctcacgtag
    12541 accacactga acatgatgta atggtaattg taacagaaca aggatatgct gatttaagag
    12601 gtcttgcacc tagagaaaga gctataaaaa taatagaaaa ttgtgcccat ccagattata
    12661 aagaacaact tagggattat ttaaatagag cttgtaaaag tggagcaaaa caaacacctc
    12721 acatattgga cgaagcttta tcatggcata gtaaatttat gagtacaggc acaatgaaaa
    12781 aggctgaaac ttttaaaagt gcattataaa atatttaata aattggaggt agattttaaa
    12841 tggaaaaagc agtggaaaac tttgaagatt taagtaagga atacattaat ggttatattg
    12901 agagagcaag aaaagctcaa agagagtttg aatgttatac tcaagaacaa gtagataaaa
    12961 tagtaaaaat agttggaaaa gtagtgtatt ataatgctga atatttagct aaacttgctg
    13021 tagaagagac tggaatggga gtatatgaag ataaggtagc taaaaataaa agcaaagcaa
    13081 aagttatata taacaattta aaagataaaa aatcagttgg tataattgat atagatagag
    13141 aaactggaat aacaaaggta gctaaaccag ttggagtagt agcggcaata actccttgta
    13201 caaatccaat cgttacacct atgagcaatg caatgtttgc acttaaggga agaaatgcaa
    13261 ttattatcac acctcatcat aaagcaattg gatgtagtac aaaaactgta gagatgatta
    13321 atgaagaatt agaaaaaata ggagctccag aaaatttaat acaaatactt gaccaacaat
    13381 caagagaaaa tacaagaaat ttaatttcat cagcagatgt agtaattgcc actggaggaa
    13441 tgggaatggt aaaagctgcg tactcaagtg gaaaaccagc acttggtgta ggggcaggaa
    13501 atgttcaatg tatcatagat agagatgtag acattaaaga agcagtacct aaaatcatag
    13561 caggaagaat ttttgataat ggtatcattt gttcaggtga acagtcagta atagtagcag
    13621 aagaaatgtt tgataaaata atggatgaat ttaaaaataa caaaggtttt atagttagag
    13681 ataaagtaca aaaagaagct ttcagaaatg ctatgtttgt aaataaatct atgaataaag
    13741 atgcagtagg tcaatcagtt catacaattg caaaaattgc aggtgttgag ataccagaag
    13801 atacaaaaat aatagtaata gaagctgatg gaccaggaga agaggatatt attgcaaaag
    13861 aaaaaatgtg tccagttata tctgcataca agtataaaag ttttgaagaa ggtgtagcta
    13921 ttgcaaaagc aaatttaaat gtagaaggaa aaggacatag tgtatctata cattcaaata
    13981 cagttaaaaa tatagaatat gcaggagaaa atatagaagt atcaagattt gttattaatc
    14041 aatgttgtgc tactagtgct ggaggaagtt tctttaatgg tcttgcacct actaatactc
    14101 taggttgtgg ttcttgggga aataacagca tctctgaaaa tcttgattat aagcatctaa
    14161 ttaatatttc tagaattgct tattatatgc cagaaaatga agtccctaca gatgaagaac
    14221 tttggggata aaatttagac aaaaaatatt cattctaaat taaagtagga atatatttta
    14281 atttagaatg aatttaagac ataaattaaa tttaaattag tttattggac taaatttaaa
    14341 atattaagag acatatagaa gaaggaagta ttataattta aaataaataa aaaaatctat
    14401 aaaagagaag gagtggtttt attatggcat taatgacagg agctcaatat attgaaagtt
    14461 tgagaaaatt aaatactaag gtttatatgt ttggtgaaga ggttaagaat tgggttgacc
    14521 atcctatgat tagaccatct ataaattgtg tagcagcaac ttatgactta gctcatgacc
    14581 cagaatatgc agatttaatg acagttactt caaatatcac aggtgaaaaa ataaatagat
    14641 ttggtcatct tcatcaaagt gttgatgatt tgataaaaaa agtaaaaatg caaagactat
    14701 gtggtcaaaa aacagcttct tgtttccaaa gatgtgttgg tatggatgcg tttaatgcag
    14761 tgtattcaac aacatttgag tgtgataaag cccatggaac taattatcat gataattttg
    14821 taaaatactt aacttatata caagaaaacg atttagtagt tgatggagct atgactgacc
    14881 ctaagggaga tagaagtcta tcacctagtg ctcaacctga cccagatatg ttccttcata
    14941 tagtagaaag aagagaagat gggataattg taagaggagc aaaggcacat caaactggtt
    15001 caataaattc acatgagcac cttataatgc ctactataag tatgactgaa gcagataaag
    15061 actacgctgt atcatttgca gttccttcag atgctgaagg agtgtttatg atttatggaa
    15121 gacaatcttg tgacactaga aaattagaag aaggtgcaga tgttgattta ggaaataagg
    15181 agtttggagg tcaagaagct ttagtagtat ttgataatgt atttgttcca aatgatagaa
    15241 ttttcttatg tggagaatgg gatttctctg gtatgttagt agaaagattt gcaggatacc
    15301 atagacaaag ttatggtgga tgtaaagtag gagtaggtga tgtaataata ggagcagcag
    15361 ctttagcagc tgattataat ggagcaaaca aagcttcaca tataaaagat aaactaatag
    15421 aaatgactca ccttaatgaa agcttatatt gctgtggtat agcatgttca tcagaaggtc
    15481 acaaaacaga agctggaaac tatcaaatag atttacttct tgcaaatgtg tgtaaacaaa
    15541 atgtaactag atttccttat gaaatagtaa gacttgcaga agatatagct ggagggctta
    15601 tggttactat gccttcagaa aaagacttta aatcagacct taaagttgga acaagtggaa
    15661 tgactattgg cgaagtatgt aacaagtatt ttaaagcttc ttcagtggct tcaacagaag
    15721 aaagaatgag aatattaaga ttcttagaaa atatatgcct tggttcatct gctgttggtt
    15781 atagaactga gtcaatgcat ggggcaggtt caccacaagc tcaaagaata atgatttcaa
    15841 gacaaggaaa cataaatcaa aagaaagaat tagctaagaa aattgcggga ataaaaaaag
    15901 aggaagcttt aaatttataa aaagttgata tataaaggga aattatatat taatgtgtat
    15961 taattaatga aaaaatatgt agtataagag atagttataa aattatggtg gtttttagct
    16021 aagtaaagaa atttttaata aggagaattt acacatgacc tgtggagtta gattttgtgg
    16081 gggctgtaac cctagatacg aaagagggaa ggctcttaca ataatcaaag agcacttcaa
    16141 tggtagggta gattttagta tagcacaaga gaacgtcgag tatgatttgc tacttataat
    16201 tggaggatgc gcaactctat gcgcatccta tcaacaatat aaagtgaaaa atgaatgtgt
    16261 taagatgtgg gattttagcc atatagaaag agttataaat gtgattgaat cagcaatttg
    16321 tgaaacttaa aaaagctaaa tggggggatt tcattgagtt ggcaagaatt gtatcaaagt
    16381 aaattatgta gtgctacaga agctgtaaaa cagattaaaa acggtgatac tgtagtattt
    16441 gcacattgtg taggtgaacc tcctgcacta gtagaggcta tgattgaaaa tgctgaacaa
    16501 tataaagatg ttgagattaa acatatggta agcttgggta gtggtggata tactgctaag
    16561 ggaatggaag ctcattttag agtaaatcca atgtttgtaa gtggaaatgt aagaaaggca
    16621 attgaaaatg gagatggtga ttttacacct gcattcttcc atgaagtacc aaagttgctt
    16681 agagaaaaaa gattaaaatg tgatgttgtt ttagcacagg taactccacc agatgaacat
    16741 gggtattgta gcttaggaac atcagttgat tatacttatg aagccattaa aactgctaga
    16801 acagtaattg ttcaagtgaa tgaccagttt cctagaacct atggtgaggt agtccatgta
    16861 tcagagtttg actatatagt ggaaaaatca caacctttat ttgaactaca acctgcaaag
    16921 attggagaag tagaagaagc aattggtaaa aattgtgctt cattaattga agatggttct
    16981 actttacagc tagggattgg tgggatacca gatgcagtta tgttattttt aactgataaa
    17041 aaggatttag ggattcattc agaaatgatt tcagatggga ctttggccct ttatgaaaaa
    17101 ggtgttataa atggtaaata taaaaatttt gataaagaaa aaatgacagt taccttctta
    17161 atgggtacta aaaaattata tgactttgca aataataacc ctgcagtaga ggtaaaacca
    17221 gtagactatg tgaatcatcc agcaattata atgaaacaac ataagatggt ttctataaat
    17281 tctgctatac aagttgattt aatgggacag gtagttgcag aggctatggg acttagacaa
    17341 tttagtggtg ttggaggtca agttgacttt atacgtggag tgtctatggg tgaagatgga
    17401 aaggcaatta tagctatgcc ttcaataact acaaaaaaag atggtacagt aatttcaaaa
    17461 atcgtatcta ttgtggatga aggtgcacct ataacgacat caagaaatga tgttgactat
    17521 attgtaacag aatacggtat agcagaatta aaaggaaaat ctcttagaga aagagcaaga
    17581 aatttaataa atatagctca tccaagtgta agagaaagtc tagcagtaga atttgaaaag
    17641 agatttaagg agaaatatta ataatagagg ggtgttaata atggaagcac ttagagtagt
    17701 tccaaagata ttttactttg atacatttaa agaatttaat gaagaattta aaataggaaa
    17761 aaatgattta gtaattacta atgagtttat atatgagcca tatatgaaac ctcttggaat
    17821 agatacaaat ttaatttttc aagaaaagtt tggaacaggt gaaccatctg atgaaatgat
    17881 agactccatg acaaaggaaa tgaagaaata caattttgat agaattattg catttggagg
    17941 aggaacaata gttgatatat gtaagatatt agcacttgat gttccagaaa agtctattga
    18001 tttatttgaa ggagaagtat tacctaaaaa agtaaaagag ttagtagtag ttcctacaac
    18061 atgtggtaca ggtagtgaag taacaaatgt agctattgca gagttaaaat ctaaacatac
    18121 taaaaagggg cttgcagtag aagaaacata tgcagactat gcagtgttaa ttccagagac
    18181 agtaaaagga ttaccttaca aattctttgt tacaagttcg gtagatgctc ttatacatgc
    18241 tattgagtct tatctttctc caaaagcaag tccatttaca gagatgtatt ctctacaagc
    18301 aataaaaatg attatggatg ggtataagaa gatagttgat aaaggagaag aagagagatt
    18361 taatcatctt ggagactttg tacttgcatc aaattatgca ggaattgctt ttggaaatgc
    18421 tggttgtgca gcagttcatg cactatctta ttcaataggt ggagctttcc atgttgcaca
    18481 tggtgaagca aattatcagt tctttactga agtatttaag atgtattcta gaaaaaaacc
    18541 taatggaaaa attataaaat gtacaaaaat tttagcagat gcccttgaat gtgaccctaa
    18601 ctgtgatgtt tatggagaat tagaaaagtt tttaaataaa ttaatagcta aaaaagcttt
    18661 gagagaatat ggaatggtgg aaagtcagat agatgaattt acagatagta caatagcaaa
    18721 tcaacaacga ctacttgcta ataattatgt agagcttagt agagaggaaa taagagagat
    18781 atttgcaaat ctttattaat acaatttttt atttatatgt atagtcaaca taaacaatgc
    18841 taattatatt tatgtacaaa taaaaataag ggaacttaaa ttttaattaa agttaagttc
    18901 tcttattttt atatggagtt aaatataagt atatttttaa tttaaaatat gcttatattt
    18961 aagtctatta ctttatttga aacaatttgt agaatttcaa attccttgac agtaataagt
    19021 aagtaattgt ataatcaaac tgcaatatat taataatatt agtgaaaatg aaaggattat
    19081 aaaatatgta taaatcagaa aatttttcaa aaaaatctgt attaataaat tcatttttaa
    19141 gtaaattttt aaaaataatt ggatggggtt tggtaagctt atttacatta tcagccttag
    19201 tactttcaac tatttccatt ataattggat gggctgtaat acttcctatt caagtatgtg
    19261 cagtatttct acttatagga ggagtttgta tttttaaagg taagagatta ggagaccaaa
    19321 tttctcgtta taaaaaatat tgtacaatta taaacaatag aaatattatt ccagtagaac
    19381 ttattgcaga aaacacatct aaatcattaa attttataat aaaagatgta caaaagatga
    19441 tagacaaaaa ctactttata aatacttata ttgataaaag aaataatcag attgtgttaa
    19501 caaatgaaga ttttattcca ccaagatatg aagatgtagc atatgaagtc aaagaagaag
    19561 aaaagactga cgaatccaat gaagttgata caattataaa taaaggaatg aattatctaa
    19621 cacaaataaa agaagctaat aaaaatatta aatcagaatc aatgggcgat aaaattgtac
    19681 aagtagaaga tgttacaagt aaaatatttg atgttgtaaa acatgaccca tcaaaactta
    19741 ctcaaataca aaaatttatg gattattatc ttccaaccac tttaaaacta ttaaattcat
    19801 atcatacatt ggaagaacaa ggaattgata gagaaaatat aacaacaact atggaaagta
    19861 ttgaaaatac aatgaatacg attgtagtag cttttgaaaa tcaattggac tatctgtttg
    19921 aagatgaagc cattgatatt tctacagata ttactgtttt agagaatatg ttagtccaag
    19981 aaggattaac gagtggtaaa ttttaacttc aatagaaaaa atattctgtt atttttaaac
    20041 aattaattag tcataaagta taaaatgtgt attatataag ttaatcagta ctttatgaag
    20101 gaagaggaga gaaagataca atgaataatt tagatgatat accagttatg ccaactttga
    20161 cacttgaccc atttggagaa gaatcaaaca ctaatgatat agataattct gacttattaa
    20221 tgaaaaagga tgaaaaagac ccagaagaag aaaaactgtc tgaatctgaa cgcaagatgg
    20281 taaaggaatt tgcggaaaaa atagatatta caaatacaaa tatgattttg caatatggag
    20341 ctggtgctca aaaaaaggta gcaagttttt ctgaaacagc tttaaagagt gtaaaaacaa
    20401 gagatttagg agaagttgga gatatgctta caaatctagt tactgattta aaagcatttt
    20461 cagcagatga aaaagaacaa agtggttttt taggaatttt taaaaaggca aataataaaa
    20521 tttcaaattt aaaaactaag tatgatagtg cagaggtaaa tgtggataaa gtaagcaaag
    20581 agcttcaaaa gcatcaggtt aaaatattga aagatatagc aatgcttgat aagatgtatg
    20641 aattgaactt agcttactca aaagagatta ctatgtatat tatagcagga aaacaaaaat
    20701 taaaggatat gaaagaatat gagatgccaa agttgagaga aaaagctcgt ttatctggtt
    20761 caacagaaga tgctcaatct gtaaatgata tggtttcact ttgtgacaga tttgaaaaga
    20821 aaattcatga cttagaatta actagaatgg tatcgcttca aatggctcct cagataagat
    20881 tggttcaaaa tagtaataat ctgatggcag aaaagattca atcaactata gtaaatacca
    20941 ttccattatg gaaaaatcaa atagtgcttg ctcttggaat ttctcactta aatcaagcta
    21001 tgaaagcaca gcgtgaagtt agtaatatga caaatgagct tttattaaag aatgcagaaa
    21061 ctttgaagat gggaacaata gaaacagcaa aagaatcaga aagaggaatt gtagatatag
    21121 acacaattaa gaaaacgaat caatctctaa tatctactat tgatgaggta gttaaaatac
    21181 aacatgatgg tagaataaag agacaagaag cagaagttga attatcaaaa atagaaaatg
    21241 aacttaaaag taagcttctt gaatttacag ttaaataaaa attatactaa tataaaaaga
    21301 gactgtctca aaatagagat aagtctcttt ttcattgttt aaatgattaa agtatattta
    21361 tattataaat ttctattatc ttttaacata acatcatgag ctccaccttc aactatacta
    21421 gtagaagata caagtgtaag tttagcattt ttttgaagtt caggaatgct aagtgctcca
    21481 cagttacaca tagtagaacg aactttactt aaagaaagag ttacattgtc tttcaaagaa
    21541 ccagcataag gcacataaga gtcaacacct tcttcaaaag aaagtttctt atctccacca
    21601 aggtcatatc tttgccagtt tctagcacga gcagaacctt ctccccaata ctctttcata
    21661 taactaccat ttatattaac tttgttagtt ggactctcat caaaacgaga gaagtatcta
    21721 ccaagcatta taaaatctgc acccatagct aaagctaaag ttatatgatg gtcataaacg
    21781 ataccaccat cagagcaaat tggaatataa atacctgttt tttcaaagta ttcgtctcta
    21841 gcttgagcaa cttcaattat agaagtagct tgtccacgtc ctataccttt ttgttcacga
    21901 gttatacata tcgaaccacc accaatacca actttaacga agtcagcacc agcttcagct
    21961 aggtaaagga atccttcgcg gtcaactaca tttccagctc ctaccttaac tgtatcacca
    22021 tatttttcac gaatgaaatc aagtgttatt ttttgccact cagaaaaacc ttctgaagaa
    22081 tctatacata aaacatcagc accagcttca actaaagcag gaacacgttc agcaaagtct
    22141 ctagtattaa taccagctcc aactacatat ctcttagaag aatctaaaag ttcaagtgga
    22201 ttttctttat gagaagagta atctttacgg aataccatat acataagatt accattatca
    22261 tcaagtacag gaagagaatt aagtttgtgg tcccatataa tattgtttgc ttcctttaaa
    22321 gtaacatctt tgttagcata aactatagat gacataggag tcataaattc actaacttta
    22381 gtatctaaat ccatacgact tatacgataa tcacgacttg caacaatacc caataattta
    22441 ccagttgaag ttccatcttc agtaacagct actgtagaat gtccagtttt ttcttttaag
    22501 tcaagtatgt cttttaatgt gttatcaggt ttaatatttg aatcacttac aacaaaacca
    22561 gctttgtggg atttaacacg tgctaccata gcagcttcat tttcaatagt ttgagaaccg
    22621 tatataaatg aaataccacc ttctttagca agtgcaatag ccattttgtc atcagaaaca
    22681 gattgcataa tagcagaagt caaaggtata ttcatataaa tatctgcttc ctcgcccttt
    22741 ttaaatttag taacaggtgt ttttaaactt acattagcag gtatacaatc agtagaagag
    22801 aaacctggta caagaaggta ttcactgaaa gtatgtgatg gtgtatcaaa ataaaacgcc
    22861 attttttaat cactccttat atttttatac tttttataat tgatagtatt aattataact
    22921 tattctttaa ttttattaaa gtattttgtc aaatattgag atttttgaaa aatttaaact
    22981 attttaatta aatttgttta atatatattg ccctataggg taaagttgta tattaaataa
    23041 tgtttagtgt ttaatagtag ctaatttaaa attaatcttt agtgattcat ataattacat
    23101 ttgtatagtt tacattgata atattactag agagtgcata aacctcaaaa atacagataa
    23161 attaaatcta tattagtata tccaaaagta catttaataa att
//
LOCUS       DAEMHW010000047        22402 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEMHW010000047 DAEMHW010000000
VERSION     DAEMHW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.047"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(121..894)
                     /locus_tag="KPK67_001567"
     CDS             complement(121..894)
                     /locus_tag="KPK67_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBG2458555.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="KPK67_001568"
     CDS             complement(894..1280)
                     /locus_tag="KPK67_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2458556.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="KPK67_001569"
     CDS             complement(1305..1622)
                     /locus_tag="KPK67_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBG2458557.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="KPK67_001570"
     CDS             complement(1634..1894)
                     /locus_tag="KPK67_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBG2458558.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="KPK67_001571"
     CDS             complement(1894..2190)
                     /locus_tag="KPK67_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG2458559.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KPK67_001572"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KPK67_001572"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBG2458560.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KPK67_001573"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KPK67_001573"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBG2458561.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KPK67_001574"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KPK67_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBG2458562.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="KPK67_001575"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="KPK67_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBG2458563.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="KPK67_001576"
     CDS             complement(8083..9189)
                     /locus_tag="KPK67_001576"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBG2458564.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="KPK67_001577"
     CDS             complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="KPK67_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBG2458565.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="KPK67_001578"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="KPK67_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBG2458566.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="KPK67_001579"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="KPK67_001579"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBG2458567.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="KPK67_001580"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="KPK67_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBG2458568.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="KPK67_001581"
     CDS             12283..12990
                     /locus_tag="KPK67_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG2458569.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="KPK67_001582"
     CDS             12959..13588
                     /locus_tag="KPK67_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBG2458570.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="KPK67_001583"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="KPK67_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBG2458571.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKEIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15177..17069
                     /gene="mnmG"
                     /locus_tag="KPK67_001584"
     CDS             15177..17069
                     /gene="mnmG"
                     /locus_tag="KPK67_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBG2458572.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKSAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="KPK67_001585"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="KPK67_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBG2458573.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17944..18729
                     /gene="noc"
                     /locus_tag="KPK67_001586"
     CDS             17944..18729
                     /gene="noc"
                     /locus_tag="KPK67_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBG2458574.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18850..19623
                     /locus_tag="KPK67_001587"
     CDS             18850..19623
                     /locus_tag="KPK67_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG2458575.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAIIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19625..20488
                     /locus_tag="KPK67_001588"
     CDS             19625..20488
                     /locus_tag="KPK67_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBG2458576.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20521..21663
                     /locus_tag="KPK67_001589"
     CDS             20521..21663
                     /locus_tag="KPK67_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG2458577.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21797..22372)
                     /locus_tag="KPK67_001590"
     CDS             complement(21797..22372)
                     /locus_tag="KPK67_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBG2458578.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDTSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
BASE COUNT         8047 a         3231 c         3227 g         7897 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaaa
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaagtg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgacgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcagtt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctatagcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctagtaagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcat gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tgtattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt tcaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtatt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tctttagtat ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcgt taaatccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aaccgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag gatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta tagttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctgagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatgg cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctattg ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttata ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttactag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaagta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaagaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga attttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac caaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatggttgg ttaaatgtaa atctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa aaagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt ctggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atagtggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaat tcctatacag gagaagatgt tatagaaatt aattgccatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 gggttcttta tcaaagaaga taagagaatt aagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga aattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataagag atactgatga tattgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaagaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaatttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggc ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gtcttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggcg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt acgatttagt gataaaacat cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 ttgagaactt atttagtgca gggcaattta atggaacttc tggatacgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccatttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagagaa agaatttgaa agactaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttgga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt aggaaaaagc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaagc tagacagaaa ttagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatctgg tgtttctcca gctgatatat cagttttact tatataccta gaacaaatca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgaatgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aaaaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaaaatgaag actataggga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaagtgat ggaaacacca gttaagtacc ctagaaaagc tggtaaacca
    17761 gccaaaaatc ctattaagta atgaaaaatt tattattggt aagcttaatt atttctcagg
    17821 aaataattaa gcttactgta aaaaaacaaa aataaaggaa ttattaaagt agatgcagaa
    17881 ataaatttgt atgtacatat agagaacaaa aataatttat atctaaaata agagaggtat
    17941 gttatggaag ataaaagagt tatggagata cctatagaag atgtagttcc aaatccatat
    18001 caaccaagaa aaatattttc acaagtttca ttggaggaat tgagcaattc tataaaggta
    18061 tatggtataa ttcaacctat aacagttaga gcaaaagatg gcaaatatga actaattgca
    18121 ggagaaagaa gattaagagc agctaaatta gcagagctta aaaccatacc agcaattata
    18181 aataatatga acgatgaatc ttctgctgtt ctagcgttat tagaaaattt gcaacgagaa
    18241 gacttaaatt tcattgaaga agcaattggt tatgaaaact taataaaaga acatgctttt
    18301 actcaacagc aattagcaga aaaattgggc aaaaatcaat ctactgtagc aaataagctt
    18361 agaatattaa agcttccaaa cgatataaaa atgaagctaa tagagaataa tttaacagaa
    18421 agacatgcta gagcatttct taaactacca agtgaagatt taatgcaaag tgtgttagac
    18481 aaagtcataa aaaatgaatt gactgttaaa aaaacagaaa aattgataca agatgtatta
    18541 gaagaaacta aagttcaaga agagcctgat aaaaaacaga atataaaggg tgctatgagt
    18601 ataagaattt acataaatac tataaagcag gcctttgatg ctatatcaaa tacaggaatt
    18661 gatgctaagt ataatgaaat agataaggga gattatatgg aagttgttgt aaaaatccct
    18721 aaaaaataga gattactgct taccagtaat ctttttttgt aatataaatt gatttataga
    18781 cattatttat acttaaaaaa cacctattca acatttaaaa ttaatttaat tcttaaaggg
    18841 gggaacatca tgggcaaagt tatagctgta ttcaatcaaa agggtggagt cgggaaaact
    18901 acaacaaatg taaatttaag tgctagctta ggaactttgg gaaagaaaat attagtatta
    18961 gatttagacc cacaaggaaa tacaactagt ggatatggta ttaataaaaa cgaagtagaa
    19021 aatacaatct atgaaattat gttagatggg ttacatatta aagaagcaat aataagtact
    19081 gaatttgaga atgtagatgt agttccatca gctactgaac tatcaggtgc agagatagag
    19141 cttacatcta aaactaatag agagtatatt ttaaaaaatt ccataaaagc gataatagat
    19201 gaatatgatt atatattttt agattgtcca ccctcactgg ggatgcttac aataaattgt
    19261 ttaactgctg tagatagtgt tttaattcca atacaatgtg aatattatgc tttagaaggt
    19321 gttagtcaat taatggagac tataaaatta gtaaaatcaa gattaaatgc tgatatagaa
    19381 attcaaggtg ttgttttaag tatgtttgat ggaagagcta atttatcaat acaagtagtt
    19441 gaagaagtta agaaatattt taaaggcagt gtatatacaa ctttaattcc aagaaatgta
    19501 agacttgcag aagctccaag tcatggaaaa cctgtaattt attatgataa aagatgtaga
    19561 gggtctgtag cttatttgga gctagcagaa gaatttatag atttagaaga ggaggaatgg
    19621 tagtatggag aataagtcta aaagaagtaa tagattaggt agaggtctta gtgcattgat
    19681 accagaaatt aaaggagaaa cttcagaaaa agaaatagtc aacatagata tagacaaaat
    19741 atatccaaac gaagttcaac ctagaaagca atttgatgaa gaaaagataa aagtattgtc
    19801 ggactctata aaaaattatg gtgttttaca gccaatagtg gttaaaatgg atgaaaataa
    19861 taaatatatg ataatagctg gagaaagaag atttagagca tcaaaacttg caaataaaaa
    19921 tcaaatacct gctataatta aagatataga tatgaaagat ataatggaga tagccttaat
    19981 tgaaaactta cagagggaag atttaaattc aatagaagag gctctagcat ataagagttt
    20041 aatagagcat tacaatgtaa ctcaggaaga aatatcagaa gctgttggga agagtagacc
    20101 tcatataact aatacactaa gacttttaaa tcttggacaa gatgttatag aaatgataga
    20161 cagtggtaga atcacagcag gccatggtaa ggctttattg agaatagcag ataaagattt
    20221 gcaacttcaa atagcgaaaa aaatagaaga agaagaactt tctgtaagag aagtcgaaaa
    20281 tatagctaaa aaaatatctg aaaataaaca agaagaacct aaaaaatcta aacctaagga
    20341 tgtatttatt ttagatgttg aagataaact tagaaatata tttggaacaa aggtgaatat
    20401 ttctaaagga aagaaaaaag gtaaaataga gatagaatac tataatgatg atgatttaaa
    20461 tagtattgta tcaatgcttc ttgaatagaa caaaaatata aaacagagag gaagattgtt
    20521 atgatttatt tagataatgc tgcaactaca tatccaaaac cagaaagagt gtataatgct
    20581 gttttagatt gtatgaaaaa ttattgtgca aatccaggta gagcaggcca taaacttgct
    20641 atgagagctg ctagggagat atatgataca agagagaata tagctaagtt gttcaatgta
    20701 agtaatccta tgaatatagt atttacatct aatgcaacag attctttaaa tttagcgata
    20761 aaaggtgtat tacaagaagg agaccatgta ataacaacgt ctatggagca taattcagta
    20821 ataagaccta taaaagcatt ggaaaaaagg ggtattgaaa atactgtagt aaaatgcgat
    20881 tatgagggat ttttagatta tgaagattta gaaaaatcaa taaagaataa tactaaattg
    20941 attgtaacaa cacatgcgtc taatgtatgt ggaactttaa tagatataaa aaaagttgga
    21001 gaaatagcta aaaaacataa tatactattt ttagtagatg catcacaaac agcaggtgtt
    21061 tatgatatag acgtaaatga atgtaatata gatatgttgg caatgccagg tcataaatgc
    21121 ttatttggac ctcaaggaac aggtatatta tatgtaagag aagggttgaa cttaaatata
    21181 ttaaaagaag gtggaacagg tagtaaatca gaagaaatag ttcaaccaga gttattccca
    21241 gataaatatg aatcaggaac tcataatact ccaggtatag caggtcttaa ccaaggtata
    21301 ctatttatat ttgaaagagg aatcaacaat ataagacaac atgaagaaga attatgtcaa
    21361 tatatgatag acaaattaga ggaagtacca gatataaaga tatatggacc aaaggacagt
    21421 aaaaaaagag catcagtaat agctctaaat ataggagata tggattctgg agaggttact
    21481 ttcttattag atagtgatta taatatagcg actagatcag gaatacattg ttcaccacta
    21541 gcacatacca ctttaggtac tttaaaacaa ggtgcagtaa ggttcagtat aggatatttc
    21601 aacacaaaag atgagataga taaagcagtg gaagctttga aaaaaatttc aaaaaataaa
    21661 tagcatgaga tttaataata tagaaataaa aaaaggggat atttcaaaat aaaaatttga
    21721 atcatctcct tttttgtaca aaaaagaaaa ggggttccta agaacctcta atctatttga
    21781 taatagtttt aagaaattac tttaagtaat ctccttcttc aagaactata tgaccacttt
    21841 cgtaattaga accatcaaca gtaattttta tttgttcaac attcatattt tttatgtaag
    21901 tacgagcaac tgaatcaagc attaaagttt ctgcatcact acctaagttt gactttgtaa
    21961 agtcagagct aacatctaat ataccagttt ttacaccatc aatgtctttt gtagtaaatg
    22021 aatttaattt tgcagattct ggaacaactt taagtttttg aagttcttca aataaagtgt
    22081 tctcatcaat tttatttaaa tcaactttat tttcaattaa atctgtctta tctacatcaa
    22141 aagagtaaat agtagctgat tgagttttag tttcttcttt tggagtaggt ttagctttgt
    22201 tattaggaac ctctttttca gtattgtttt cttcttttgg ttgctcaaca tttgtattgc
    22261 tcttagtttc ttttttagat gtatcacttc cattttgagc attagtacaa ccaactgcga
    22321 atatagatat agataatata cttaacacta acattatttt cttatttttc ataaaaattt
    22381 ctcccctctt aaaaactatt ct
//
LOCUS       DAEMHW010000048        22260 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEMHW010000048 DAEMHW010000000
VERSION     DAEMHW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.048"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..171)
                     /locus_tag="KPK67_001591"
     CDS             complement(<1..171)
                     /locus_tag="KPK67_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2458579.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVESTLYPVITMIILFLIII
                     NNQVRFFSLSNEN"
     gene            355..699
                     /locus_tag="KPK67_001592"
     CDS             355..699
                     /locus_tag="KPK67_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBG2458580.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            730..2331
                     /locus_tag="KPK67_001593"
     CDS             730..2331
                     /locus_tag="KPK67_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBG2458581.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFSKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2350..3450
                     /gene="dprA"
                     /locus_tag="KPK67_001594"
     CDS             2350..3450
                     /gene="dprA"
                     /locus_tag="KPK67_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBG2458582.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3483..5570
                     /gene="topA"
                     /locus_tag="KPK67_001595"
     CDS             3483..5570
                     /gene="topA"
                     /locus_tag="KPK67_001595"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBG2458583.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5735..6520
                     /gene="codY"
                     /locus_tag="KPK67_001596"
     CDS             5735..6520
                     /gene="codY"
                     /locus_tag="KPK67_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBG2458584.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6627..7892
                     /locus_tag="KPK67_001597"
     CDS             6627..7892
                     /locus_tag="KPK67_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBG2458585.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7923..8693
                     /locus_tag="KPK67_001598"
     CDS             7923..8693
                     /locus_tag="KPK67_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2458586.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8802..9248
                     /locus_tag="KPK67_001599"
     CDS             8802..9248
                     /locus_tag="KPK67_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG2458587.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9266..10459
                     /gene="nifS"
                     /locus_tag="KPK67_001600"
     CDS             9266..10459
                     /gene="nifS"
                     /locus_tag="KPK67_001600"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBG2458588.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKGNMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10513..10953
                     /gene="nifU"
                     /locus_tag="KPK67_001601"
     CDS             10513..10953
                     /gene="nifU"
                     /locus_tag="KPK67_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBG2458589.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11242..12321
                     /gene="mnmA"
                     /locus_tag="KPK67_001602"
     CDS             11242..12321
                     /gene="mnmA"
                     /locus_tag="KPK67_001602"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBG2458590.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQVDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12464..12702
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12770..15409
                     /gene="alaS"
                     /locus_tag="KPK67_001603"
     CDS             12770..15409
                     /gene="alaS"
                     /locus_tag="KPK67_001603"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBG2458591.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15434..15691
                     /locus_tag="KPK67_001604"
     CDS             15434..15691
                     /locus_tag="KPK67_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBG2458592.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15701..17872
                     /locus_tag="KPK67_001605"
     CDS             15701..17872
                     /locus_tag="KPK67_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458593.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            18078..18503
                     /gene="ruvX"
                     /locus_tag="KPK67_001606"
     CDS             18078..18503
                     /gene="ruvX"
                     /locus_tag="KPK67_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBG2458594.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18581..18868
                     /locus_tag="KPK67_001607"
     CDS             18581..18868
                     /locus_tag="KPK67_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBG2458595.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19150..19608
                     /locus_tag="KPK67_001608"
     CDS             19150..19608
                     /locus_tag="KPK67_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG2458596.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19725..20462)
                     /locus_tag="KPK67_001609"
     CDS             complement(19725..20462)
                     /locus_tag="KPK67_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG2458597.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20679..>22260
                     /locus_tag="KPK67_001610"
     CDS             20679..>22260
                     /locus_tag="KPK67_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBG2458598.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWA"
BASE COUNT         9098 a         2287 c         4153 g         6722 t
ORIGIN      
        1 attctcattt gataaagaaa aaaatctaac ttgattattt attataatta gaaataatat
       61 tatcatagtt atgaccggat acaatgtact ttcaacattt ttcaaatagc ttacaactaa
      121 tataaataaa gataaatatc gtataaaagc aactaatttt tctctgttca ttaaaggcac
      181 tcctccaaaa aaataaactt ttcctatgaa aactagaata atatcataaa agaataaagt
      241 caatagaagt atttattaat attgatggaa ataaaatcaa tccaaaaact tgaatagtaa
      301 aattgtatac tgaccataat ttaaaagtga aaatttaatg tattggagtg aataatgaat
      361 aataaagaaa aaggtgattt tggagaaaaa gtagcagtta attatttgct atcaaaagga
      421 gcaaagatac tagaaaaaaa ttacagatta aagatagggg aaatagatat aatagcaaaa
      481 atggaagatg aaattatatt tgtggaggtg aaatcaaggt ctaacataaa gtttggctac
      541 ccttgtgaat ctgtaagctt caaaaaaaga aaaaagataa ttggagttgc aaattattat
      601 attataaaaa ataacttaaa taacactccc ataagatttg atgttataga ggtatattta
      661 ttagaaaaaa gaataaatca cataatgaat gctttttaaa aaagataatg tttttggaaa
      721 ggattaaata tgttaagtat tattaattca agcaacttag taggaatcga cagcttttta
      781 gttaaggttg aagtagatgt aagcaatgga atacctagtt ttaatattgt tggtcttcca
      841 ggcaaagaaa ttaaagaagc aagagaaaga gtaaagtcag caattcttaa tagtggatat
      901 aaatttccaa gcacgagaat agttgtaaat ttatcaccag cagatataaa aaaagaagga
      961 gcttttttag atttatctat aagtataggc cttttaagag agttaataaa aaaagatgaa
     1021 aattacataa gagaaagtat gtttatagga gaattatcat tagatgggaa aataagaaaa
     1081 gtaagaggta tattgcccat aattatgggt gcaaaaactc agaacataaa acgtattttt
     1141 ataccaattg agaacataaa agaaagtcta tttgtagatg aaattgatat aattcctatt
     1201 aaaagtttaa aagaatgtgt cgatttttta aacgaagaga taaaagtcga taaagtaagg
     1261 ataatgtctt ttttggatga taagtcaaga aaagagaacg aagaattaga aaaagataat
     1321 agttatatag attgcaaata tactaagatt aataatgaag aaagtaaata tgatgaagac
     1381 tttaaagatg taaaaggaaa ttattttgta aagaggagtg cagaaattgc tgctgctgga
     1441 aatcataata tgtttatgat tggacctcca ggctcaggaa aaactatgat tgcaaaaaga
     1501 gtaaggacaa ttttaccaga tataagtgta gaagaaatga tagaagtaag taaggtctat
     1561 agtatattgg gtatgataaa tgaaactaaa ggaataattg ataaaagacc ttttagagca
     1621 cctcatcata caactacaaa gcaatcttta attggaggag gaatggatgc aagacctgga
     1681 gaaattgctt tagcacatag aggtatctta tttttagatg agatagccga atttgataga
     1741 aaaatacttg agactttaag acaacctata gaagatggat atgtaaatat atccagagta
     1801 aagtacagtg caaaatatcc ttgtagagta ctgttagttg cagctatgaa tccatgtcca
     1861 tgtggttatt atatgtcaga gacagagtgt agatgtagaa gtaacgaaat tgatagatac
     1921 ataaataaaa tatcaggtcc attgttagat aggtttgata tttttgtaga agttaattct
     1981 ataaaatatt cagattttaa ttctttaaaa caagaagaaa gctctcaaaa aataaagaga
     2041 agagtagaaa atgctagaaa aatacagata aatagattta agaaggataa tataaaaaat
     2101 aatagtgaaa ttaaagctta caatttattt aaatattgta aactggaaaa agaagcttca
     2161 aaaacggctg agatgatatt tagtaaatac aacctaagca gtagaagtta tacaaaactt
     2221 ttaaaaatgg caagaacaat agcagattta gaagaaagag acttgataaa ttcacagtgt
     2281 attatagaag ctttttcatt tagaaaggct tattatagtt attttaaata gttttttgag
     2341 gtggatttta tggaaaaaag agatacttat ttatggttaa aatcaattgg tggaataaca
     2401 acaaaaacaa ttgaaataat agaaaatgaa atagtaaata ttgaagatat atttgatttt
     2461 tctgaaaaag aaatatataa tttaaaaaat ataagcttga atattagaaa aaatatagta
     2521 aaatatagag gtcacgctta tttagaaaat ataaaagaac tactttacaa aaaagcgatt
     2581 aaatatattt gtaaatatga taaagaatat cctgaaaatt taaagaatat atacaacgct
     2641 ccaaagttat tattctacaa aggtgacata ggtttagtta ataataattt taatatagct
     2701 atagtaggtt caagaaaacc tacagcctat ggaattaatt gtgcaaaaac aataagttgt
     2761 caactatccc aatatggagt taatatagta agtggattag ctataggaat agatgcatac
     2821 tcgcacatag gttgtatgag tggaaaatct aaaactatag cagtacttgg ttcaggagta
     2881 gacaatcctc ttccaaaaca aaatttacat ttatcaaata aaatattaga aaatggggga
     2941 ttattattat cagagtataa tattaactcg acagtagccc cgtaccattt ttctaataga
     3001 aatagaatta tcagtggttt aagtgatgga gtagttgttg tagaagcagc aataaaaagt
     3061 ggtgcactaa tcacagtaga ttttgctctt gaacatggaa aaaatgtttt tgcaatacct
     3121 ggtaatataa attctcaaat gagtagaggt tgtcataaaa ttataaaaga aggtgcaaaa
     3181 ttaattgaaa atatagatga tattttaaat gaatataaca tatttaatat tattgataaa
     3241 aaaataaatc aaaaatatga taatataagt ctaaatgcca agtctaagca aataattgaa
     3301 gctataaaaa gagaaggaaa cttgcatata gatagtattt gtgattatac tggtatagaa
     3361 ataaagtatg taaattcaat aatcaatgaa ttagtactaa atgagttagt agtagaaatg
     3421 aataataaaa cgtacagttt aaatgtataa attaagatta ggtagtgggg gtgtatggtt
     3481 ttatggccaa aacattagtc atcgtagaat cgcctgcaaa agctaaaact atagaaaaat
     3541 ttttagggaa aagtcattac acagttaaag catcagttgg tcatgtcaga gatttaccta
     3601 aaagtaaatt aggtgtagat atagaaaata attttgaacc tcaatatata aatataagag
     3661 gaaaaggcga tgtaataaaa gaattaaaaa aagaagctaa aaaatctaaa caagtatatc
     3721 tagcaactga ccctgataga gaaggtgaag ctatatcatg gcatttagct catatactaa
     3781 atttagatga atctgaggat tgtagaatag agtttaatga gataactaag gatgcaataa
     3841 aaaaagcaat taaacatcct agaaatattg atataaattt ggttgatgca caacaggcaa
     3901 gaagagtgct agacagactt ttaggttatc aaataagtcc tatattatgg caaaaggtta
     3961 gaaaaggtct tagtgcaggt agagtgcaat ctgttacaac aaagttaatt tgtgatagag
     4021 aaaaagaaat aaaggcattt attccaaaag aatactggac aatagatgtt gaggctaaga
     4081 cagtaaaaga caaagatata actttgaaat tttatggtaa aaatgatgaa aagattgaat
     4141 tagaaaatga agaagctgta aatgaaatac taaaagaaat ggaaggtaaa gatttaaagg
     4201 ttgagaagat agaatctaaa tcaagacgta aatcagctcc aaaaccattt acaacaagta
     4261 tgcttcaaca agaaggagca aataagttat tttttactac taaaaaaaca atgatgatag
     4321 cacaagaatt atatgaaggt atcgacatag aaggtgaagg tactgttggt cttatatcat
     4381 atataagaac tgactctaaa agaatatctg atgaagcaaa agaaaaagct aagggctata
     4441 tcgtaaacaa tttaggagaa aactactata aaaacactgg tgataaaaaa gaaaaaaagg
     4501 atactaaaaa ggttcaagat gcccatgagg caataagacc aacctcagtt gatagaactc
     4561 ctgagagtat aaaagcctca ttgagcaaag accagtacaa gttatacaat ttaatttgga
     4621 gaagatttgt agctagtcaa atggaagatt cagtgtttga tatactaaat gtagaatgta
     4681 aaataggcga tattgtattt aaggcaactg gttcaaaaat gaaatttgat ggttatacca
     4741 aggtatataa tttttcagaa agagaagaca aaattttacc aagtatagaa aaaggagata
     4801 tattaaatat agaaaatata ttgccagacc aacattttac acaaccacca gcaagatata
     4861 cagaagctag tttagtaaaa acactagaag aactaggtat aggtagacca agtacgtatg
     4921 ctccaacaat agctactata ctgaatagag agtatgttga aaagcaagga acaagtctat
     4981 gtccaacaga acttggaata atagttactg atattttgga aaataatttc cataaattta
     5041 tagatgtaga ttttactgct gatatggaaa gtaaacttga tgttatagaa gaaggcaata
     5101 cagattggaa aaagattgta tcagaatcat atgcaccact taaagaggct attgaagaag
     5161 ctcttgaaaa tatagaaaaa gtaaatatgg atgaagaaac tgatgagatt tgcgagaact
     5221 gtgggtctaa tatggttatc aaatatggta gatttggaaa gtttatggct tgtaaaaact
     5281 atcccgattg taaaacaact agaccaatta tgaataaaat tggtgttaaa tgtccaaaat
     5341 gtgaagaagg agatattata cttagaaaat ctaaaagagg taaagctttt tatgggtgtt
     5401 ctaattatcc aaactgtgac tttgtagctt ggaataagcc aacaggagag ttttgtgaga
     5461 agtgtggttc atatatggtt gaaaaagtga ctaaatctga aacaaaaaca gtgtgttcta
     5521 ataaagaatg taataaagag acaaaagagc ataatgacgg aaaagagtga aaattgaaaa
     5581 aaatcacata attctcttga aaaaggttcc aatctatgat aatctttcaa gtgtaaacaa
     5641 ctaatagaaa aataaatttt cattaaatgt gaaaataata ttagaaaata taaaaggtta
     5701 ttaaaaggcc taaatttcat gaggagatga ttaaatggca agtgaagtgt tacaaaaaac
     5761 aaggaaaata aataaaacat tacaaacaag tggtggaagc agtgtctctt ttgatttact
     5821 ggccggagca ttgggcgacg ttttaagttc taatgtttat gtagtaagtg caaaaggtaa
     5881 agtactaggt cttcatttaa atgatgttca agacagttca gttatagaag atgagtatac
     5941 taagcaaaag aaattttcag atgaatatac tcaaaatgtg ttaaaaattg atgaaacatt
     6001 agaaaattta aatggtgaga agatattaga aatctttcct gaagaacatg gaagattaca
     6061 aaaatatact acagtagttc caatattagg aagcggtcaa agattaggaa cattggtact
     6121 ttcaagatat tcaaattcat tcaatgatga tgatttagta atagctgaat acagtgcaac
     6181 tgttgttggt cttgaaatat taagagcaat aggtgaagaa ttggaagaag aaatgagaaa
     6241 gaaagctgta gttcaaatgg caataggcac tctgtcctac tccgagcttg aagcagttga
     6301 acatattttt gctgaattgg atggaaaaga aggtctactt gtagcaagta agatagctga
     6361 tagagttggt ataactaggt ctgtaatagt aaatgcactt agaaaatttg agagtgcagg
     6421 tgtgatagaa tcaagatcat taggtatgaa aggtactcat ataagaatac ttaatgacaa
     6481 acttacagat gaattaaaaa aattaaaaaa caatcaataa tctatatttt ataggtttag
     6541 attagaaaag gtatctattt caaatagata ccttttttag acgaattatt aaggtaatga
     6601 taagtatatt aaaaaggagt gagaaaatgc ctttagcaga taaaataaga ccaaaaacaa
     6661 tgaatgatgt aataggtcaa agtcatataa taggtaatgg aaaaatattg tctaagatat
     6721 tacaatctaa ttttttacca aatatgatat tttttggtcc tcctggtgtt ggtaaaacta
     6781 cagtagcgga aataatagca gaaagaagca ataaaagctt ctataaaata aatgctacta
     6841 attcatcttt agaagatata aaaaaggtga tatcagagct tggaagtata aataatgtaa
     6901 atggagtcct attatatata gatgaaatac aaagttttaa taaaaaacag caacaatcaa
     6961 tattagaatt tatggaaaat gggagtataa ctttgattgc tagcactaca gaaaatccat
     7021 atcattatgt atataaagct cttttaagta ggtctactgt atttgaattt aaacctttag
     7081 aaaaacttga tatagaaaaa ggactgaaaa gagctgttga agtattaaat gaagatagct
     7141 atatggatat tgagtgtaat aataatgcta ttgaagatat agcaattctt tctgatggag
     7201 atatgagaag agccttaaat attttagaag tggtagtcta ttcaactaaa ccaaataagg
     7261 ataatataac ttatatagat tcagatgtaa taagggcatc tacatttaat aaaattataa
     7321 actatgacaa aaatggtgat agtcattatg atattttaag tgcatttcaa aaatctataa
     7381 gaggaagtga cccacaggca tctatacact atctagcaag acttataaaa ggtggggatt
     7441 tgataagtat atgtaggaga cttttagtaa tagctagtga agatataggt ttagcttatc
     7501 caaatgctat tgtaattgta aaggcatgtg tagactcagc tatgcaactt ggatttccag
     7561 aagcgaaaat accattagca gaagctacta ttcttttggc tacatctcca aagtctaatt
     7621 ctgcatgtat agcaataatg aaagctatgg atgaactgga taaagagttt gttaaggata
     7681 taccaaatag tataaaggat gctcactatt ctggttctaa agaaataggt aggggttgtg
     7741 gatacaaata tcctcataat tttaaaaatc attatgtaaa gcaacagtat ttaccagata
     7801 gcataaaaga tagtatttat tacataccac aagaaaataa aactgagaaa aatataaaaa
     7861 aatatttaga atatctaaat agtgattttt agactgaact ttatgtatcg ggggtagata
     7921 ttatgattat aaaacttaac tcaacttatc acagtagagt tatgaaatac ttaaaaaaag
     7981 aacctgagta caatttgttt ataatagggg atattgaaag atatggatat ggaaataatt
     8041 ttttaaatat atgggcagat gtgggtgaac atggtgaaat aaaggcaata ttacttaaat
     8101 attttgaatt tatgatgttt tattctgatg gagagtatga tgttgaaggt ttttataatc
     8161 tacttcgtaa tactaattac gaagaaatat ctgggaaaat atgtgctgta gatgctttag
     8221 caaagagatt aggacttaat aatttaaagg tagttgattt ttgcaaactt caaactaaaa
     8281 agtttttgat agataataat tgtaatgcaa aggttaagag aattagactt ggaaatctaa
     8341 aaaagacagt aaaactttat gacttaatag atgaatttca cagtacaact ttagagaatt
     8401 taaaaaatgg actaaggaca ggtagaggat attgtataga gattaacaaa caggtggtgt
     8461 caatggcaaa gagtacttca gaaaatagga ctcatgcaat gataattgga gtaggaacac
     8521 atcctaaata tagagctaaa ggacttgcta caaaatgcct aattaaacta tgttcagagt
     8581 tactgagaga aaacaaaatt ccttgtttgt tttatgataa tgaagaggct ggaaagatat
     8641 ataaaaaact aggatttgaa aacattggaa aatggggaat ttattcaaaa taaaaaatta
     8701 aaattaatct tgacaaaatt agtagggtat aataaaatta tcttgaaagt aattcctact
     8761 aaaacagtat gaattagatt tctatataga ggtgataata tatgaagttg tctactaaag
     8821 gtagatatgg actaaaagca atgtttgaat tagcattaaa tcaagacaat ggaccagtat
     8881 cattaaaatt tattgctaag aagcaaaaaa tatcagatca gtacttagaa caaatatttt
     8941 catctttaaa aaaatcgggt ctagtaaaaa gtgtgagagg tgctcaagga ggttatttgc
     9001 tttcaaagaa tgctgaagac ataacagtag gagatatact tgtagtatta gaaggaccag
     9061 ttgcattatc tgactgtgta ctagatgaag atgtatgtga aaactcaaac atgtgtgtaa
     9121 caaaaatagt ttgggaaaag atgaaaaagg gtatagaaga tgttatagat tcaattactc
     9181 ttaaagatat gataaatgac tataacaaaa ataaattaga aaatgatata acaaatatta
     9241 aaaaatagat ttggagatga atgtaatgga aaaaagaaga ttgtatatgg attattctgc
     9301 aacaacacct attaaaaaag aagtattgga tgcaatgatg ccatacctaa cagattattt
     9361 tggtaatgca tctagttttc acacattcgg aagagaagca aaagatgctt tggataaagc
     9421 aagagaacaa gtagcagccc ttataaatgc tgaacctagt gaaatatatt ttacagcagg
     9481 tggttcagaa agtgataact ggacactaga gggtgtagct tatgctaata aaaacaaagg
     9541 taatcatata ataacttcaa aaatagaaca ccatgcaata cttcatacat gtgaatactt
     9601 agcaaaacat catggatttg aaataactta tttagatgtt gatagcgaag gaaaagtaga
     9661 cttaaagcaa ctagaagatt caataaaaga tactactata cttataagta taatgtttgc
     9721 aaataatgaa ataggaacta tacagcctat taaagaaata tccgaaatag ctaaaaaaca
     9781 taagatatta ttccacactg atgcagtaca agctacaggt aatattccag tagatgttaa
     9841 agaattagga atagatttga tgagtatgtc ttcacataaa atatatggac caaaaggtgt
     9901 aggagcttta tacataagaa aaggtgtaag acttcataac tttgtccatg gtggagcaca
     9961 agaaaaaagc aaaagagctg gtacagaaaa tatacctgca atagttggat atggaaaagc
    10021 agcagaatta gctaagggaa atatgcaaaa tcatgtagaa actttaactc gtcttagaaa
    10081 taagttaata gatggtgtgt tagaaagaat accttacaca agagtaaatg gtagcttaga
    10141 aaatagattg ccaggaaatg caaactttgc attccaattt atagaaggtg aaggaatact
    10201 tttattatta gatatgttag gaattgctgg ttcaagtggt tctgcatgta cttctggttc
    10261 attagaccct tcacatgtac ttcttgcaat aggtttacca catgaaatag cacatggttc
    10321 attaagactt acagttggag attttacaac agatgatgat atagattata tactagagaa
    10381 cttaccaaaa gtaatagaaa gacttagaag catgtcacca ctttatgatg atgcgaagaa
    10441 acaaggttta gtaaaataga cataaagata aagcataaaa aatagacata aattgagtag
    10501 gaggaaaaga taatgcaata tagtgataaa gttatggaac attttatgaa tccaagaaat
    10561 atgggagaaa ttgataatgc aagtggtgta ggtgaagttg gaaaccctac atgtggagat
    10621 ataatgaaga tatttttaga catagatgga gatgtaataa aggatgttaa gtttaaaaca
    10681 tttggctgtg gttcagctat agcaagttct tctatggcaa ctgaaatgat aaaaggaaaa
    10741 actataaaag atgcattaga acttacaaat aaagctgttg cagaagcttt ggatggttta
    10801 ccaccagtaa agatgcattg ttcagtttta gctgaacaag ctgtaaaagc agctcttata
    10861 gattatgctc aaaagaacaa tatccatata ccagaattgg atggatatgt tatagatgat
    10921 gctcatgacc atgatgttga agaagaagaa taaaaataag tgattgtgtt atgagaagtt
    10981 tacaatgatt aaaaagaagt actaatgata taattaggac ataaaaaata gctgttttta
    11041 aaatggctat tttttattgc ctaaattaag tattttaact tgatttagag gttaattagt
    11101 taacaaaata atccataaga ttcaaataat aaagatgatt atttttttag aaaatagaac
    11161 aagctatatt ttaaataagt acaatacttg ctctatatgg tataataata agtaatgcaa
    11221 aagatgaggt gaaatttgtt tatgaataaa aaagtaatga taggtatgag tggaggagtt
    11281 gatagctctg tagctgcata tcttttaaaa caacaaggat atgatgtcat tggtgttact
    11341 atgaagttgt ggcaagttga tgatgttgtt gaaattgaag gtggttgttg ttctttatct
    11401 gctgttgaag atgctagaag ggtagcaaat aaaataggta taccatttta tgttttaaat
    11461 tttagagaag tgtttaaaga aaaagtaata gattatttta tagatgaata tttggaagga
    11521 aaaactccta atccctgtat agcttgtaat aaacatataa aatttgatga tttttataag
    11581 aaggctagac aaataggttg tgattatgta gctacaggtc actatgcaaa gatagaaaag
    11641 gatgaaagta caggaagata tttgctaaaa aaatctgtta cagataaaaa agaccaaact
    11701 tatgcacttt ataatcttac acaagagcaa ctagagcata ctttattgcc aataggtgac
    11761 tatgaaaaag atagagtaag agaaattgct aaggaaatgg gtatggcagt acacaataag
    11821 cctgatagtc aagaaatttg ttttgttaaa gataatgact atgcaaatta tgtaaaaaaa
    11881 cattctaaaa agcgtattga agaaggtttt tttgtagata ctaaaggcaa tatacttgga
    11941 aaacataagg gtatattata ttatactata ggccaaagaa aagggcttgg aattactttt
    12001 ggaaaaccaa tgtttgtaat agacataaat ccaataaata ataccatagt tcttggagac
    12061 aatgaagatt tatttaaaaa ggaacttatt gctaaagatg ttaactttat atcaatagat
    12121 acgttagaag aacccttaag agttcaagcc aaaataagat attcagcaaa accatcacca
    12181 gctactattc atagagtagg agaagatact attaaaatag tttttgatga agctcaaaga
    12241 gctattacta aaggtcaatc tgtagtaatg tatgatggag atattgtagt aggtggaggt
    12301 ataatcgaga aaagtttata aaaacatcta aaaaattaaa aaaataagca ataaagtata
    12361 ttaagagtaa ataaagttta taaaataaat ttattgtata tgaaaatata gtattgatta
    12421 attttgtaaa atttagtaat ataattagta tattaattaa aaaacaaata cagtgataag
    12481 gaatagtaaa atatttgatg tcttacagag agagggaaat gctggaagcc cttagaatat
    12541 gatatttgaa gcagcctttt agtagcaatc ttgaacaatt tatcttttat aaagaagatt
    12601 taagtagaga ttgacggtag ttacgttata gctaaggttt tccgaagata tcatactttg
    12661 tatgataatt agggtggtac cgcgattgtc ctcgccccta taaatttatt tatagggacg
    12721 aggtttttta tttaaacgaa aaaataaaat ttattaggag gaacatgata tggaaaagat
    12781 gggattaaat gaaataagaa gtaagttttt agaatttttt gagtcaaaag gtcattatgt
    12841 agcaaatagt tattctttag taccaaacaa tgataagagt ttgttactta taaactcagg
    12901 tatggcacct ttgaaaaact acttttcagg tgtagaagta cctccaagtg tgagaatgtg
    12961 tacatctcaa aaatgtataa gaacaggaga tatagaaaat gtaggtataa ctgcaagaca
    13021 tgctacattc tttgaaatga tgggtaactt ctcatttgga gattacttta aaagagagtc
    13081 aatcaaatgg ggatgggaat ttgttacaga atggctaaat ataccagaag ataagatatg
    13141 ggtgactgta tatgaagaag atgatgattc ttatgatata tgggcaaaag aaatgaattt
    13201 cccagaagaa agaatggtta gacttggaaa agatgataat ttctgggaaa taggtacagg
    13261 tccttgtggt ccttgttcag aaatatattt tgatagagga gaagaatatg gatgtgacaa
    13321 tccagactgt aaaccaggct gtgattgtga taggtattta gaattttgga accatgtatt
    13381 tactcaattt gatagagatg aagaaggaaa ttatagtcta ttagaaaaca agaatataga
    13441 tacaggtatg ggtcttgaaa gaatgggatg tattatgcaa ggcgtagaca ctatctttga
    13501 agttgataca attaaatcta tactagaagc agttgagaag ttaacaggag taaaatatgg
    13561 agagaatcca aaaaatgata tatcaataag aataataact gaccacataa gagctgtaac
    13621 attcttagtt agtgatggag tgcttccatc aaatgaagga agaggatatg ttcttagaag
    13681 actccttaga cgtgctgctc gtcatggtaa gttattagga gtaaaagaat tatttttaca
    13741 gaaactaata gatgaagtta taaaagtaaa tgataaagca tatccagttt tagttgaaaa
    13801 agaaagttat attaaaaaag tagttggaat agaagaagaa aaattcaatg aaactataga
    13861 ccaaggaaca gaaatattga actcttatat agaagtatta aaaaatgaag gaaagactgt
    13921 cctaagtggt caagaagctt ttaaactata tgatacttat ggcttcccta tagacttaac
    13981 taaagaaata ttagaagaag aacatctatc tgtagatgaa gaagctttta atgaagaaat
    14041 ggaaaagcaa aaagaaagag ccagaaatgc tagaggaaat atggatggag aaagttggaa
    14101 ggaagaccct ctatcaaaat tagaatctac agttgacagt acatttaatg ggtatagtga
    14161 aatttatggt gaaggaacta ttgaagctat agttaaagat gatgagctag ttcaaagtgc
    14221 agaagaaggc gataaggtat ctatagtact agataacact actttctatc ctgaaggtgg
    14281 aggacaagtt ggagattgtg gattgataac taatgaaaac ttagttttag aagtattaaa
    14341 tactaaaaaa ggtgctaata atagtataaa acatataggt ataataaaat caggaagaat
    14401 aagtaatggg gacaaagtaa agacactcgt agatagagaa actagaatgt cagctgcaag
    14461 aaatcacagt gctacacatt tacttcataa ggcattaaga gaagtattag gagaacatgt
    14521 taatcaagct ggttctttag taactccaga aagacttaga tttgatatta ctcactttga
    14581 agcgatatca aatgaagaat taaaagtaat agaagaaaaa gtaaataatg ttattttatc
    14641 atctttagac ataaaatgtg atattatgaa tataaaggaa gctaaagaaa aaggagctac
    14701 agcactattt ggtgaaaaat atggtgatga ggtaagagta gtttctatgg gagattattc
    14761 tacagaactt tgtggaggaa ctcacttgac aaatacttct caagtaggaa tgttcaaaat
    14821 attatctgaa ggtggagtag cagcaggtgt aagaagaata gaagcaataa caggaaaagc
    14881 tgtttatgaa tacctaaaag aaagagatgg aattatctct gaagtttgtg taaacttaaa
    14941 atctaaagaa gataatttaa ttcaaagaat aagttcttta ttagaagaaa ataaaaactt
    15001 atctaaagaa ttgcatgata tgaaagctaa gatgagttta caatcagttg attctatttt
    15061 tgattctaaa gttgaagtaa atggagttaa tttgataact aataaatttg aaggtatgga
    15121 tatggataca ctaagagaaa cagcagataa tctaagagat aagcttggtt ctggtgtagt
    15181 agtacttgca aatgtagtag atgataaagt taactttgta gttactgcca ctaaagatgt
    15241 attagataag ggaatacatt caggtaatat tgttagagaa gttgcaaaaa tagctggagg
    15301 aaaaggtgga ggaagaccta atatggcaca agcaggagct agtgatgtat ctaaggttga
    15361 ccaagcttta agctatgcaa gtgaggttat taagacacaa gttaaataac attcactcaa
    15421 ggaggttttt agtttggaaa aagatttaga ctacactatg aaatttgaag gaataccaga
    15481 agataggatg agtgtaggag atactattga ttttgtatat aaagcattag ttgaaaaagg
    15541 atataaccct ataaatcaaa ttatagggta tctcctttct ggtgattcta gctatataac
    15601 tagtcataaa aatgcaagag ctataattaa aaaatttgaa agagatgaga tacttgaaga
    15661 agtaatcacg cactatctga atagaaagta ggtcaaataa atgagaaaaa agattatgtc
    15721 gttgtttgca atagtcatta tgatttttgt atcaactgct agctatggtt ttgctgaaga
    15781 aaaaggcaag gtcatattta ttgatatgaa taggaccaat cttggaagta tgatgaatat
    15841 tccaatcttg aaaaatgaga tagaaaagag aggctatgta gctctcatga atataagagg
    15901 ggatcaaggt actgatgata gaaggtctta tgcaagtatg ggtgcaggaa caagagctag
    15961 tgtaactact gaagactata tcaattttca aaaagtagac caagatactg ctaaagcttt
    16021 tgaagtcgca acaggtcaaa aagctaaaga aataaatgat ttaggcatta atagatctat
    16081 aaatgaaaat cttgagtatg gagaatatgg agcaaattta gggtcattag gaaaaacttt
    16141 atcagaaaat ggtttaaaga catctgtaat tggaaatgct gatataattg aaaataatca
    16201 attagttaaa aacagaaact tatgtttagt agctatggac caatatggaa ggattgattc
    16261 aggtaatatt gataatatta ataagaaaga ttctaaaatg ccatttggaa tatctacaga
    16321 ttatgataaa ttactagatg aaactaaaaa atcctataaa aatagtgatg ctatatttgt
    16381 agaacttgga gatacatata gattagatat gtatagatta aatctaaatg ataatactta
    16441 tgatacaatg aagaaaaata ttgtaaaaaa tataaataaa tatcttgaac aagtattttc
    16501 tatggttgga gaaaatgaca ctgtttatat agcaagtgca tttccaagtg acctagatta
    16561 taagaataag agaagacttt ctccaataat aaaatttagt ggagaaggga aaggtctact
    16621 ggaatctgcc acaacaagaa aggctggaat tgttgccaat ttagatgttg gagtagatat
    16681 actaggtaca tttggtcttg aaaataaaga gatggtaggt agaagttata ctttagtaaa
    16741 taaagaggac aatattagct atctaaaatc actttatgaa aggatagtgt ctatatcttc
    16801 aataaggtca actattataa atggctttgt aggcgtaata tctgcttctt gggtaattgc
    16861 catgtttgcg attttcttta gaaattatat gccacataaa gagcgtgtat tcaaagtact
    16921 taaggagttt ataaaattag gcataataat gccactatca tttatgttag ctcctttgat
    16981 gaattttaaa actccaattt caatatcttt gggaatattt gcgactacgc taatattata
    17041 cttgttaggt agaaaattat ttaaggacaa tgatataaag cacatgggat tttttgcaac
    17101 acttactatt ttagtcatag ttatagattc tgtatttgga acatacttaa tgcaaaataa
    17161 cataatgagt tatgatgctg tcataggagc taggtactat ggtgtaggaa atgaatatga
    17221 aggtgtaact atagcaagtg cagtatttgg cttagcagta ttattacatt ataagaaggt
    17281 atctaaatta cttgttgtaa ttttttcatt aataatactt ataactagtg catatccttc
    17341 aatgggtgca aatgttggtg gagctatatc agaatgtgta gcatatttgt tgtttataat
    17401 gcttatatat gacattaaaa tagattttaa gaaagctgta ttattagctg tttcagctgt
    17461 agttgtcgtt tttgcatttg cagctttaga tatactttca ggtagtgaat ctcatttagg
    17521 tatgtttgta cagagaattt tcctaaatgg accaggagag atattacaaa catttggaag
    17581 aaaaattcaa atgaatatgc aactagcaca gacaagtgta tgggtaaata ttttacttgt
    17641 aggaattgga gtaattgcag tgcttatatt taaaccatct agacacttta gaaagataat
    17701 gaataatttt ccaatcttgt ttaaagggtt tattgcatct atggttggtt gtataattac
    17761 tcttttagtt aatgattctg gaatagttgc tgcatctaca gcatctatat atatacttat
    17821 accactaata ataataagta taaatatgat aatatttgaa aaaaataact aaaaaaattt
    17881 ttactgttca tataggattt aaataaaaaa tgcaatatga ttattaatgt aaactggatg
    17941 cttacataaa aaatcatatt gcaattttta tgattgatta tgtaaaattc tatgaagcag
    18001 taagcctgtg accttagtta tgagaggttc agaatatgta tttaatctat agaatataaa
    18061 tagataaagg agaaactatg ttagatggaa gaataatggg tcttgatgtt ggtgataaaa
    18121 ctattggagt tgctgtgagt gatttgatgg gactaactgc acaaggtgta aaaacaataa
    18181 aaagagttgg caagaaaaaa gatattgaag agctaaaagc aataataaaa gaaaaacaag
    18241 taaataaaat tgtatcagga ttaccaaaaa atatgaatgg aactttaggt ccacaaggag
    18301 aaaaagtaat caagttttgt gaattggtaa aagcagaaac aggtattgat gtagagtttt
    18361 gggatgagag gctttctaca gtagcggcag agaggtcttt attagaagca gatgttagta
    18421 gacaaaaaag aaaaaaggtt atagatatgt tggcagcagt aataattttg caaggatatt
    18481 tagattttaa aataaattca taaaagaact ataaaattga atacttatga tatataatat
    18541 aaataataaa aataaaaata aatttgaggt gaatgacatt atggaagaaa atataataaa
    18601 tttaatagac gaaaatggcg ttgaaagcca atttgaaata atattaacat tagaagcaga
    18661 aggaaaagag tatgcaatat taatgccttt agatgatgaa gaagctgaag aagctttaat
    18721 ctttagaatt gatgaggatg aagaaggtga aatcttaata cctttggaaa gtgatgaaga
    18781 gtatgaaact gttgtagctg tgtatactgc tataatggaa gaagaaggtt taaattacga
    18841 cgaagatgaa tcaaatggac ttaactagat aagtaattaa gtttgtatat aaaaatcaga
    18901 tatttattat atttgcttta attaaacctt attaattgta aaagcattta tagtatatat
    18961 ctgttttttt gttttttatt aaaaatacca aaaaatttat gaattttggt gaataatata
    19021 tcttttatgg gtatatattt atattatgag aataaattaa atttatttaa atgtcttgac
    19081 aaaaaatgaa ttatcaatta caatattatt taataaagat tatcaattga aaatagtaag
    19141 ggtgatgtta tggcaaatac aatggattta ttaaaagaca agttaaaaga aactggtttt
    19201 aagattacgc cacaaagaag agcaatagtt gaaatattgc ttaaacatga ccattcacat
    19261 ctaagtagtg aggaaatcta tgatttagtt agggttgact gtcctgaaat aggcttagct
    19321 actgtatata gaacaatgca gctattggat gaaataggat taatatcaaa actaaactta
    19381 gacgatggat gtataagata tgagattagt ctacataaag aagactgtca taatcatcat
    19441 catcttattt gtaaaaattg tggaaagata atggaagcta aagaagacct tttagacaat
    19501 atagaaaaag agatacaatc attgtacaaa tttaaaatac ttgatcatga tgttaaattt
    19561 tatggactat gtgacgagtg taatggagta agcgattcag aagaataaac ttctattgct
    19621 taatgtcgat ggaagaatag tatagaactt ttatattcta tgctattctt ccatttttta
    19681 ttataaacca tctacaaaat gcaaatatac agttttcaag atgcttatag ttttaatatt
    19741 gaaaatataa ttaatcctat aagtacaaaa atgaatgatt tttctatgaa attactaaat
    19801 ggtcctccaa cagttaaaaa acctaaagaa attttctttt cgcttagcgg ataaaaaatt
    19861 ggtattcctt gctttgtaaa taaatctcct aaaaacatat gacttgcata accaatcgta
    19921 ccataaaaag ataaattatt aatattagtg attaattcta tctgttttag taaaaaatat
    19981 actattatag ttgcaaatat gctatgagaa aaacttctgt gctttaacca gggaaaaatt
    20041 gacaacatta gacaaaaaat tgtaaaatat atttcaactt ctatgatgta taagcatata
    20101 gctaatagta taaatattaa tgatagaaaa acttttctta gaaatgtgtg ggttagtttt
    20161 gcttcaataa ttataattgc aacaaatgta attattgcgc ttaaataaaa attatcattt
    20221 atatttattg aaataaagaa aataataata ttaacagcat atataaaaat tctatatatg
    20281 tatttggata cattgttttt tagaatgaaa tttgagacca tagaattaga tttatccaaa
    20341 tcgggtagta cagcaaaagt agctgaaact aaaatatcaa ctaaagatat aggaatttta
    20401 aatattaaag atgtttggat agctgttaag attcctattg cacaatgagt tttaccacgc
    20461 ataaatcctc ctaaattatc cgatattaaa tcatataata tttagaggat atcaaaataa
    20521 aataggtttg tcaaaataat acgttaaatg tattttaaat atagcttaaa atatgatacg
    20581 aattatggta caataagaat aactggtagc gagtgcgtga tttgatatta tttatgattg
    20641 tagaacgtgt taaaaaatta aatagacaga aggagatgat gcaattgttc aaaaagaaca
    20701 ccaacaagat aaaggtaatg gccttgggtg gacttaacga agttggaaaa aatatgactg
    20761 ttgttgagta taaagatgaa ataattgtta tagatgctgg acttagtttt ccagaggatg
    20821 aaatgttagg agtagatata gttataccag atataactta tttagtaaaa aatagggata
    20881 aaataaaggg tatatttatt acacatggac atgaagacca tataggagct cttccatata
    20941 tattaaagaa aataaatgta cctgtatatg gggcaagact tagtataggt cttatacaag
    21001 taaaacttaa agaacataaa atgaataatg ttaagttaaa tgttataggt cctagacaag
    21061 ttataaagct tgataatatg gaagtagagt ttttgaaaaa taaccatagt ataccagatg
    21121 cttactctat tgcaatacac acagaccaag gtataatata ccacacagga gattttaaga
    21181 ttgacttgac tcctattgat ggagatgtta tggatatgca tagaatctgt gagttgagca
    21241 aaaaaggagt tcttttaatg ctggcagata gtactaatgc agaaaaacca ggatttacta
    21301 tgtctgaaaa gactgttggg gtaggtcttg acgaattatt tgcaaaagga aatggcagaa
    21361 gaattatagt tgctaccttt gcatccaaca tacacagatt acaacaaata ataaatactg
    21421 ctgaaaagtt taatagaaaa gttgctatat caggtcgctc aatggtcaat gtagttggag
    21481 ttgcaaaaga attaggatac ctagatattt ctgatgacat gcttatagat ttaaatgata
    21541 tatgtaagta tgaagatagt gaattagtga taataacaac tggttcacaa ggtgaaccta
    21601 tgtcagctct tgctagaatg gctttttcag agcacaaaaa agtagaaata aagagtggcg
    21661 atttagttat aatatctgct catccaatac caggaaatga aaaattaata tcaagagtaa
    21721 tcaatttctt atttgaaaaa ggagcagaag tagtatacag tgatatagct gatatacacg
    21781 tttctggaca tgcctgtcaa gaagaattaa aacttataca tgcattagtt agaccaaagt
    21841 tttttatgcc agctcatggc gaatatagaa tgttaaaaag acatgctgaa atagctgaac
    21901 agcttggtat ggataaagaa aatatttttg tcatgcaaac aggagatgtt ttagaattag
    21961 ataaaaactc tgctaaggta gcaaaccgta tacaaacagg aaacatatta gttgatggtc
    22021 taggagtagg cgatgtaggt aatatagttt taagggatag aaagcattta tcagaagatg
    22081 gtcttatgat agttgtagtt actatttcca aggatgaggg taaggtctta gcaggtccag
    22141 atataatttc cagaggattc gtatatgtaa gagagtcaga agatttaatg gatggagcaa
    22201 aggatataat taagaatgtt ctaaatgaat gtgaagagaa aaatattaaa gaatgggctt
//
LOCUS       DAEMHW010000049        22176 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEMHW010000049 DAEMHW010000000
VERSION     DAEMHW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.049"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            180..1013
                     /locus_tag="KPK67_001611"
     CDS             180..1013
                     /locus_tag="KPK67_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG2458599.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDQMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1106..2038
                     /locus_tag="KPK67_001612"
     CDS             1106..2038
                     /locus_tag="KPK67_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2458600.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2090..2572
                     /locus_tag="KPK67_001613"
     CDS             2090..2572
                     /locus_tag="KPK67_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2458601.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2584..3453
                     /locus_tag="KPK67_001614"
     CDS             2584..3453
                     /locus_tag="KPK67_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG2458602.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3496..4251
                     /locus_tag="KPK67_001615"
                     /pseudo
     CDS             3496..4251
                     /locus_tag="KPK67_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
     gene            4293..5114
                     /locus_tag="KPK67_001616"
     CDS             4293..5114
                     /locus_tag="KPK67_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HBG2458603.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5119..5880
                     /locus_tag="KPK67_001617"
     CDS             5119..5880
                     /locus_tag="KPK67_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBG2458604.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6041..6298
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6411..6650
                     /locus_tag="KPK67_001618"
     CDS             6411..6650
                     /locus_tag="KPK67_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG2458605.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            6954..7949
                     /gene="ilvC"
                     /locus_tag="KPK67_001619"
     CDS             6954..7949
                     /gene="ilvC"
                     /locus_tag="KPK67_001619"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBG2458606.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VMIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8002..9690
                     /gene="ilvB"
                     /locus_tag="KPK67_001620"
     CDS             8002..9690
                     /gene="ilvB"
                     /locus_tag="KPK67_001620"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBG2458607.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            9901..10578
                     /locus_tag="KPK67_001621"
     CDS             9901..10578
                     /locus_tag="KPK67_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG2458608.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            10747..12783
                     /locus_tag="KPK67_001622"
     CDS             10747..12783
                     /locus_tag="KPK67_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBG2458609.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTSDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFDMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNVT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(12974..13540)
                     /locus_tag="KPK67_001623"
     CDS             complement(12974..13540)
                     /locus_tag="KPK67_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2458610.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKND"
     gene            13828..14763
                     /locus_tag="KPK67_001624"
     CDS             13828..14763
                     /locus_tag="KPK67_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2458611.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            15228..16442
                     /locus_tag="KPK67_001625"
     CDS             15228..16442
                     /locus_tag="KPK67_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2458612.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(16862..17384)
                     /locus_tag="KPK67_001626"
                     /pseudo
     CDS             complement(16862..17384)
                     /locus_tag="KPK67_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            17740..18537
                     /locus_tag="KPK67_001627"
     CDS             17740..18537
                     /locus_tag="KPK67_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG2458613.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGIKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEAISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            18647..19237
                     /locus_tag="KPK67_001628"
     CDS             18647..19237
                     /locus_tag="KPK67_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBG2458614.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            19303..21000
                     /locus_tag="KPK67_001629"
     CDS             19303..21000
                     /locus_tag="KPK67_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG2458615.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            21214..22053
                     /locus_tag="KPK67_001630"
     CDS             21214..22053
                     /locus_tag="KPK67_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBG2458616.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         9052 a         2181 c         3823 g         7120 t
ORIGIN      
        1 acattctata caattataca gaaattatac agcataataa tcaccgaaat aacaaatttt
       61 aaaaattaag caaaactttt tgtcgaagtt caacgtctat atatatgtag tgtaaatttt
      121 tgggataaaa tactacaaaa atataaatgt aaaaatatag taatattgga ggtcgaacga
      181 tgagaaataa aaagaaaaaa ataaatagaa agaagttgta tttgctttta agtgctattg
      241 tagtgtgtct ggcttttata gtagttggtg ttcgcgtcag ctctttaaat atgaaggaaa
      301 atgaaattag tagaaatgca aaactgtcaa gttctactat tacagatata gtatctaata
      361 catcaataga agttagtaaa gggccaagta aaagttctgg aaaaatagca tacataacta
      421 tagatgatgg tccatctaaa tttactgacc agatgataaa aactttaaat aaatacaatg
      481 ttaaagcaac tttttttatg atagatggga atatgaagga atatccacag caagttaaaa
      541 atataattaa aaatgggaac acagcaggtt ttcatagtgt atcacatgat attcacaagc
      601 tttatgttac tagtacatca gcaaaagagg aatttgatac aaatgatcaa actttttata
      661 agataactgg taaacattca aaagtaataa gaatacctta tggaagcaaa ccatatacac
      721 cacaagcctc ttatcaggct ttagttgatg ctggatataa aatttgggac tgggacttag
      781 atacagaaga ctggagatct aattcttctc aaattgtaca aaatgtaaaa aatcatatta
      841 agaatagaaa aggtgaagat aaagaccaac ttgtagtttt aatgcatgag aaaaaacaga
      901 gtgcagaagc tttagattca gttttaaaat tcttatcaga tgaaggttat gaatttgctc
      961 cagtagatca aaaccaaata ccaaaaaatt attggttacg taatttagag tagttaaaat
     1021 ataagaatat tgattgatat ttttagaaat tgagaataaa aatgagtgag gtgtaggtat
     1081 attgaataaa aaactttata taggaatggt cggaatattg tctttgatga tggttggttg
     1141 taataaaagt ttagcaaaag taaatgatgt ggaaatcaca aaagaacagt acaaaaaaac
     1201 aaaagcagtt ttatctgcta ctaataatta tataaatggg cagtctttag atgaattaga
     1261 aaaaactttg gataaaaaag gtagaaataa attagaaaat gttataatat cgtttatggt
     1321 ggataatgaa cttttatatc aagaggctaa ggataaagga ctaactccaa gtaaaagtga
     1381 agtagactca aaatatcaag aactagaaga taagatgaat ttgaatacaa gctataagga
     1441 aaaaatggac aaagcaggtg ttgataaaga ttatttaaaa caagaaatat ctagagattt
     1501 agcaatagat aaaaataaaa aggcctttga agatagaata aacataagtg ataatgatat
     1561 ggaagcctac tatacaagtc ataaaaaaga ttttaatgta gaagaagtaa gtgcttctca
     1621 gatacttata tcgactttag ataaaaataa aaaagaagta agcaaagata aaaaagaagc
     1681 tttgaagaag aaggcagata atatattgac taaaatcaaa aatggagaaa gttttgaaag
     1741 tttggctaaa aaatattcgg atgataaagc aactggaaaa aatggaggac agctaggata
     1801 ttttacaaag gatgataaaa atgctgagtt tacaaaggaa gtgttcaaat taaagaaaaa
     1861 tgaagtttca aatgtgtttg aaactagtta tggatatcat atagtcaaag ttactgataa
     1921 aagagaaaga caaaaaagtt ttaatgagtg tcagagttta ataagagaat ctattttaaa
     1981 tgaaaagtat attgagcata taaaaaaact gaatgaagat gctaaaatag acagatagaa
     2041 tttctaacag ctcaatccta gcctgtaatc ggagggagat tttacaggaa tgtttaagag
     2101 tttaaagaga acgaaagttg agaaatacat tatcgataat aaagactctt tttatagaat
     2161 agcatatagt tatacgaaga atgaagaaga tgccttagat gtggttcaag aagccatgta
     2221 taaggcacta tactcagtgg aaaatattaa agaagtaaat tatataaaga catggtttta
     2281 taaaatttta gttagaacga gtatagattt tattagaaaa aaccgtaaat ataataatat
     2341 gacagatatt gaccttatag atgagactgg agagtatgat aaatatactg atttggattt
     2401 aagaagagct ttggaagaat tacctattga atacaaatct attataatat taagattttt
     2461 tgaagattta aagattgaag aagtagcaat aatattggat gaaaatgtga atactgttaa
     2521 aactaggtta tatactgctt taaaaaaact aaaattaaaa attgaagagt agggataaaa
     2581 agaatgacaa gcaaagaaag gttaaataag ttaaaagatg agtatcataa aattccaata
     2641 cccaagaaat tagatactat aataaatcat gaaaaaatag aaaacatata tcgagagaaa
     2701 aataaaaaag taaatagatt aagatttaaa gttgcaatag cctttgcatg tatatttaca
     2761 gtattagtaa atataagtcc tgtatttgca gacaattttt ctaagatacc tgtaattgga
     2821 gcaatagtag aagtaataac tataaaaaat tatagtttaa aaagtgaaaa ttatgaagct
     2881 gagattgata ttcctaaaat acgaggttta aaagataaaa acttagaaca aagattaaac
     2941 agtagtttta tggaagatgg aaaaaggctt tatcatcaat tccaagagag aatggagaag
     3001 atacaatcat caaagaataa aggatataaa tctttaagtt tgagttatag tgtaaaaaat
     3061 aattctaaaa aatttctatc aatagagatg acaaaaaatg agatagaggc ttcatcatat
     3121 gtgagtaagg tacattatac tattgataaa aaaagacaaa tagttttgac tttgccaatg
     3181 ctttttaaag atgataagta tataaaagtt ataagtgata atataaaaga acaaatgaga
     3241 gaacaaatga aaaaggatag tacaaaatcc tattttatag atcaaaaaaa agatttgcct
     3301 gttgaagatt ttaaaacaat taataagtat caggattttt attttaataa aaatgaagac
     3361 cttgtaattt gttttgatga atatgaagta gcacctggtt atatgggtgc agttgagttt
     3421 gtaataccat ataaagtaat aaaagatttg taaattgaat agtagtttat atataaatat
     3481 tttaagaagg acaatatgac tacatataaa aagcttaaaa aatctatatt taaaattgct
     3541 atattttcaa tatgcttatt agtttcatta gtagatacaa accctatatt tgcaggtagt
     3601 tcctttaatg aaacttcaaa tagtgatacg ataaaggtgt taaatgctaa gagtatcaac
     3661 tttaaacaag acaattatga agtgaatgtg agtattccta aaataaaagg tttaaaagat
     3721 aaaactttag agaataaatt aaacaatgag tttttgcaag atgggaaaaa aatttacagt
     3781 gaatttcaaa ataaaatgaa aaagtaagat aataagggtc atagatacct aaatgtaatc
     3841 cataatgtca aaaataattc taaggacttt ttattaatag aaatgacaaa agaagagata
     3901 gaagcttcct catctataac taagaagtat tacactgttg ataaaaatag aggaatacta
     3961 ttaaatttac caatattttt taaagatagt agatatatta gagctataag tgataatata
     4021 aaagagcaga tgagaaatca aattaagaaa gatgatacaa aatcttattt tatagaccaa
     4081 gataaagact cgccagtgaa agattttgaa tctataaata agtatcaaga tttttatttt
     4141 aataaaaatg gaaatcttgt gattagtttt gatgaatatg aagtagctcc tggttatatg
     4201 ggagcagtag aatttgtgat accaaataaa gtaataaaaa gactaagata gatatataga
     4261 agatttttag atagactggt aaggagaatt atatgaatat atatgaacaa gcaataagat
     4321 taatagaaaa taatgaggat ttcgcatttg ctacaataac gtctcattca ggttcaacac
     4381 cacgtgaaac tggagcaatg atgattgtga aaaatgattc tactatattt ggctcagtag
     4441 gaggaggtag tgtagaagca gaatgtataa aacatgcaat taatgttata aaaaacagag
     4501 aatcaatgtt gtataaattt actctaaaca aatctgatgt ggcaaaacta ggaatgatat
     4561 gtggaggtac tggagaaata caaatagatt ttatagatag taaattaaaa agtaatatag
     4621 aaaaatttaa taagagacta aaggaaaata caagtaaagc atatatattt ggtgcaggtc
     4681 atatttcaag agatgttgct gtgatactat ctcttttaga atttagaaca gtagtaatag
     4741 atgatagaga agaatttgct aatcatgaaa ggtttccaga atcagaagta gtagttttag
     4801 attcttttga aaacatacca gattttccaa ctgacgaaaa tagttatata attattttaa
     4861 caagaggtca tctatatgat tcaagtgctt tagaatgggc tcttaaaaga gaggctggtt
     4921 atataggtat gattggaagt agaacaaaaa ttggattgac ttacgaaaaa ctcatgaaaa
     4981 aaggttttaa gaaggaagaa ttatcaaaag tacatgcacc aataggcata aaattgaatg
     5041 ctcaaacacc agctgaaata gctgtatgta tagctgctga acttataaat tgtagagcta
     5101 ataaggaaaa ataaactaat gaacacaggg agtaaagata ttgatacttt aaaggttaat
     5161 tttagaggga ttgaaataaa agcagtttta gtatataaaa atagaaaaaa tatttctata
     5221 aaaatagatc catttggcaa aataactgta atgagtcctc ctaaaatatc caaaaaaata
     5281 ataaaagata taattattga aaaaggagac tggatactta aaaagtcaga aaaatgtaaa
     5341 ggaagagagg aagtatataa acaaagaatg tttataactg gcgaaaaatt tctttattta
     5401 ggtgaagagt accatctagt tataaaagaa ctgttgagag attctgtcaa aaaaagtaac
     5461 tgtagaataa ctattaacga atatcaaata gttgttcaga caaatgatac aagtacagat
     5521 tttattaaaa aatctttaaa atcttggtat aagatggaaa gtgaaagaat agtattagag
     5581 agaatagatt ttcttaaatt aaattgtgaa ataatgaagc aattagttcc agcgagtgtt
     5641 aaggtaaaag aacaaaataa gagatgggga agttgtacag ctcaaaaaaa tatttatata
     5701 aactcaaaaa tatctatggc aagattagat gtaattgatt atgtaataat acatgagttt
     5761 agccatttag tacatatgaa tcactctaaa aaattctatg atttagtaaa atccattatg
     5821 cccgattata aagataaaga aaattggcta aaaataaatg gatataaaat aataatctga
     5881 aaacgattat tccaaaatat aggcgtaaag tataaaagaa gtattattgt gaaaaaagta
     5941 aataatctga aaattttaaa aaaagtcatt gacattgtaa atgataggga atataatcta
     6001 tattaacaac aaaacagaat taaaatataa aattaaaaaa ttaaaagcaa tgacagagac
     6061 aacaatacta atagtgttat ccacagagaa ccaatgttgc tgagaattgg tgttaacagt
     6121 agtataatga tcactctgga gcttttgtac tgaatatttt tagtaagtac aaacgtatgt
     6181 caacgttaca tgactaaggt taaggtaatt aattacccga cctgaaaaaa gaggggtaga
     6241 tgtatttttt atatctacaa ataaggtggt accgcggaat ataactttcg tccttataag
     6301 taatatttat attgcttata aagatgaaag tttttttatt ttaagcgata taaataaatt
     6361 taatccatat aaaattaata aaaaaataat aaaaataagg agggattttt atggaaaata
     6421 ctgtatatgc taaattaaac caaggaatag attctttttt aagagtggct atgactctaa
     6481 gaagaagaga ggttgatata caatcaataa gtatgactgt agataatatg aataattcag
     6541 gtataaaatt aatagtaaat gaagaaaaga catctttaga ttctgtctta aaccatatga
     6601 aaaaactaca tgatataaga gagataactg ttgaaagaat gagacattaa ttaaatatat
     6661 tttataaaac taaagataaa tatgcttatt ttaaaataaa aaactaaaga taaatattaa
     6721 ataagaaaaa tcttagatta aaatataagt gtcattgaat ttattagttt atcacagtta
     6781 ataaatttat tagtttgttg tagttaataa aaactgaaaa ttctaaaatt ataattgtaa
     6841 gattatataa atttagaatt taaatctgtt gaataatatt aaaacaatga taatgagtat
     6901 gaatttctaa taaaaataaa tatcataaaa ataagaaaaa gtattgggag gaaatgaaaa
     6961 tggcaagaat gtattatgaa aaagatgtgg atttagaggt tttaaaaaat aaaaaggttg
     7021 cagttttagg ttatggaagt caaggacatg cacatgcaca aaatcttaga gataacggtg
     7081 tacatgtaat gataggtctt tatgacggaa gtaaatcagc acaaaaagca aaagaagatg
     7141 gatttgaagt taagagtgta gcagaagcta caaaagaaag tgatttgaca atgatgttaa
     7201 tgcctgatga aaaacaaaag aaagtttatg aagagagtgt aaaggataac ttaaaagaag
     7261 gtcagacatt agcttttgct catggattca atatacatta taatcaagta caaccaccag
     7321 aatttgttga tgttgtaatg gtagctccta aaggacctgg tcacttagtt agaaatgtat
     7381 ttacaaaggg aagtggagtt cctgcactat ttgcagtata tcaagactac actaaaaaag
     7441 ctacagagac agttttagca tatgcaaaag ggataggagc aacaagagca ggtgttttag
     7501 aaactacttt taaagaagaa acagaaacag atttatttgg agaacaatca gtactttgtg
     7561 gtggaataag tgaacttata aaattgggat ataaaacgct tgtagatgca ggttatcaaa
     7621 aagaagtagc atattttgaa tgtttacatg aaatgaaact aatagtcgac cttatatatg
     7681 aaggtggatt tgaaagaatg agatatagta taagtgacac agcagaatat ggagattatg
     7741 tatctggtaa aagggtaata actgatgctg caaaacaagg tatgcaaaat gttttagagg
     7801 atatacaaaa tggaaaattt gcaaaagcgt ggattaaaga aaatgaagaa ggacgtgaaa
     7861 acttcctaaa aactagagaa gaagaatata atacagaaat tgccgaagtt ggtagaaatc
     7921 taagaagtat gatgtcattc ttaaaataaa tatatattaa atggattagt ttgagggaaa
     7981 gagtggtgta tagtagtgcg tatgaatgga gcaaaggtaa ttttagaatg tttaaagaaa
     8041 gagggaattg acacaatatt tggatatcct ggtggagccg tgatacctct ttatgatgct
     8101 ctatacgatt attcagatga ttttaaacat ataaggacat cccatgaaca aggtttagtt
     8161 catgctgcag atgggtacgc aagaagtact aatacagtag gagtttgttt tacaacatct
     8221 ggtccaggag caacaaatgc cataactgga atagcaacag cttttatgga ttcttcacct
     8281 atggttgtaa tttcagggca ggtgccaact agtttattag gaaaagattc ttttcaagaa
     8341 attgacataa ctggagcaac attatctatg acaaaacata attatttagt tagaaacact
     8401 aaagaattag ttcctacaat aaaagaagct tttagagttg caaattcagg tagaaaagga
     8461 cctgtacttg tagatgttcc aaaagattta tttttagcag aaatggattt tagtggtgag
     8521 gactatgatt tatgtcaaat agatgattat atggattata agagtgattt tgatctagat
     8581 gatgaaacta atattaagct tttaaatgaa gcaatagata tcataaaaga atctaaaaag
     8641 cctataatat atgcaggtgg aggtgttaaa tcttcagaca gtgaggaaat tcttgaaaaa
     8701 tttgcaacta agatagatac acccgtacta aatacactta tggggcttgg aaatatagat
     8761 agaaagaatg aactatctct tggaatggtt ggaatgcatg gaagtagaga aagtaattta
     8821 gcactttcta attcagattt agtaatagcg ataggtgcga gatttagtga tagagtgata
     8881 agtaaaagtt ctgaatttgc taaaaatgca aaaataatac acatagacat agacccatct
     8941 gaaataagta aaaatataga atctaatgta tctttggttg gagatgtaaa gctagtcctt
     9001 agtttattaa ttgaaagggt tgaaagtaag aataatagca attggaaaga agagataaag
     9061 agatttagaa agagtgaggg tgtacaaaca tatgaatttc atccacaaaa tatacttaaa
     9121 aaaataaatg aaaaatatga aacattaaag aaacccactg tggtagtaac tgatgtcggt
     9181 caacatcaaa tgtgggctgc taagtactgg aattttaaag gtaataaaag ttttataaca
     9241 tcagcagggt taggaactat gggatttggt ttaggtgcag ctattggaac taaagttggt
     9301 aatgtagata aaaatgtagt tttagtcact ggtgatggaa gttttagaat gaactgtaat
     9361 gaactagcta cagttgcaaa ttataatgta cctatgctta ttttactact caataataga
     9421 acattaggaa tggtaaggca atggcagaag ttattttcca atcaaagata ctctcaaact
     9481 gacattaatg aaaatgtgga ttatgttaaa ctcgtaaatg catataatat tgatggatat
     9541 aaggtatcca gtatggaaga attaggaaag gcgctagata tgatagattt taataaacct
     9601 gtatttttgc aatgtgatat agataaggat tatgatgttt atcctattgt agcacctaat
     9661 gatgcattgg aaaatttgat atgtaactaa aacttatact gaatattgat aaaatttttt
     9721 attaaatttt tattagtatt cagtttttgt tgtttagaga tagattggca ttttttattt
     9781 ttgcaaataa atcaatgact atataattaa gaacttatta aattttaatt atacaattta
     9841 gaatagtatt acaacttaag taaataataa ttttgaataa taatttacgg aggtatttat
     9901 atgtttaaac acgataaagc attattaaaa gaagtaaaag tagaaagacc aaatcctcaa
     9961 tatgctgttt taatgcaaga acagttgggt ggtgcaaatg gagagttaaa agcagctatg
    10021 caatacttgt cccaaagttt tagaataaaa gaccctcaaa taaaagattt atttcttgat
    10081 atagcagcag aagagcttag ccatatggaa atggtagcac aaactataaa tttattaaat
    10141 ggacatgatg ttgattataa tgcagttaat acaggcgaaa tagaaacaca tgtattaact
    10201 ggtctatcac cagtcttaat aaactcatca ggagctcctt ggactgctaa ttacgttaca
    10261 gttacagggg atttagtagc tgacttactt tcaaatatag cttctgaaca aagagctaag
    10321 gtagtttatg agtacttgta tagacaaata gatgataaat atgtaaaaga aacaatagat
    10381 ttcttactta atagagaaga agcacataat gctctattta gagatgcttt aaataaagtt
    10441 aaagatacag gttcaaatag agattttgga gttacagaag attctaaatt atatttcgat
    10501 ttgtctactc cagggccaaa tcacgatact aagatagata ttaatcctcc ttcttttgaa
    10561 aaacctttaa aaaaataatt tgatattttt tattttgttt tagtagttat aaaaattatt
    10621 tactagaaga attaattata tgaaaaatta aatatgtata gttaaaaatg ggtatacact
    10681 tattataagt gtatatctat ttgtttattg caaaacatat aataaaataa caaatgaggt
    10741 ggtattttgg acagtaatag aaaacctaat aacctaataa atgaaaaatc tccatatctt
    10801 ttacagcatg cttataatcc tataaactgg tatagttgga atgatgaggc ttttaaaaaa
    10861 gcaaaagaag aggataaacc aatattttta agtgtaggat attcaacatg ccattggtgc
    10921 catgttatgg aaaaagaatc ttttgaagat gaagaagttg ctgaaataat gaatagaaac
    10981 tttgtggcaa taaaagtaga taaagaggaa agacctgatg tagatagcgt gtatatgaca
    11041 gtttgccaag ctatgacagg tagtggtgga tggccaatga ccataataat gacatcagat
    11101 aaaaaacctt tttttgcggg aacatatttc ccaaaatatt caaggtataa tagacctgga
    11161 gttatagatt tattagagaa tgtatctgaa aagtggaata caagcaggga tatattaata
    11221 aaatctggag atgaaataat agaagctctt aaggatgatt ttggtgtaaa aaatactgag
    11281 ggtgatttat caaaagagat gttgagttca tcagttagag tatttaaggc tatttatgat
    11341 gaaaagtatg gtggttttgg taatgctcct aaatttccaa gtcctcagaa tcttatgttt
    11401 ttaatgaaat attatagtat agaaaaagac aaagatgtgt taaaaatggt ggaaaaaact
    11461 ttagatggaa tgtatagagg aggcttgttt gatcatattg gatttgggtt ttcaagatac
    11521 tctactgata aaaagtggtt ggctcctcat tttgaaaaaa tgctatatga taatgctatg
    11581 cttactatag cttttttaga tgcatataaa ataacaaaga aagagttgta taaagaaatt
    11641 gctataaaaa ctatagatta tgtagttaga gagatgaagg ataaagaagg tgggttttat
    11701 tcagcacagg atgctgatag tgaaggtgaa gaaggtaaat tttatacatt taatcctctt
    11761 gagataattg aagttttggg tgaagaagat ggaactttct ttaataatta ttttgatata
    11821 actagtagtg gaaattttga aggtaaaagt attcctaatc ttataaaaaa taaagaatat
    11881 gaaagacata atgaaaagat agctgacctt agtaaaaagg tatttgaata tagaaaagaa
    11941 agaacatctt tgcataaaga tgacaagata cttacatcat ggaatgcatt gatgatagtt
    12001 gctttaacaa aggcatatag tactttaaaa aatgacatat atttagagta ttcaaataag
    12061 tgtcttaact ttataaataa taatcttgta aatgagtcag gtagattatt agctagatat
    12121 agagatggaa gttccgacta tttagcatat ttagatgatt atgctttttt aatctgggcg
    12181 tatatagagc tttatgagtc aacatttgat atgaaatatt tagaaaaggc tttaaatttg
    12241 aatgaaagct gtatcaacct tttttgggat tatgaaaaaa gtggatttta tatatatggt
    12301 aaagatagtg aaaatttaat agcaagacca aaagatttgt atgatggagc tattccatca
    12361 ggaaattctg ttcaattata caatcttata agacttgcga aaattactgg agataataga
    12421 cttgaagaaa tgtcatataa acaattaaaa ctgtatgtgg ataatgtaaa gagttctcca
    12481 actggctaca gcttttatat gctttcattg atgtttgaac tttattctac taaggaaatt
    12541 atatgtattt ttaaggaaga ttctgattta attgccttta aagaattgat aagtgaaaat
    12601 tttataccaa atgtaacttt tttagcaaag aaatacaatg aagaaaacac tattataggt
    12661 tttttaaata actatagatt gaaggatgat aagattagtt attacgtgtg tcaaagtaat
    12721 agttgtagtc aaccaattaa tgatttacaa aaattaaaag atatgatttt aggagtagaa
    12781 taatcataat agcgaaaaag gagatgcttt aaaataaaag tctaatcatc tccttttttt
    12841 gtacaaaaaa cagaaaaaga gttcctaaga acacctaatc tatgtaatga ttgttttgtt
    12901 aaaaaataaa aatgacatta tagttatata aaatattgta ctataaaaaa gtagtattat
    12961 agatgcgata gtattaatcg tttttattta ctctatttct tctttttgca aattcttcaa
    13021 gtgcttcaga tttaatttta tctatttctt catattccat aaaattagca tcttcttcaa
    13081 atccatcttg ttcttcacca ttaaaacgat acattttttt tagtaaactc cataaggtct
    13141 ctaattcatc tttggtaaat tcgtgaaata catctgccat aaagtttatt ccagttctac
    13201 tacatgtcac cataaccttt tttcctaagt ctgtaacttt aacattaatt gctctcttat
    13261 catgaggact tggaattaca tcaacatatc catttttttc aagattagca actagtctat
    13321 ttatattttg tttacttgta cccatttttc ttgcaatatt atttaatgtt gtttcttcct
    13381 ctggtaaatg aagaattgat aaaatagtca tatactgtct tgaagtaagt ataccaaaat
    13441 atttatctcc ttctatttgt attttgtttg taagggtaaa aagagttgca tatgtttgat
    13501 tcattaaaaa gagctctttt agttcatttg aataatccat gaaatatatc tcctttattt
    13561 ttagatgttg ttttgtcgaa aacagaatat catgaattaa ttctaatgtc aatatggtgt
    13621 caaaatatat taaagtatta cttttttatg taactattaa ttaattaaat tattgtataa
    13681 ttttatgtaa aaacttctat gtaccaatga taacttctat gaggaattaa taattcttaa
    13741 attttttaga aagattttga cttgaaaata cttgtaatat aatatataaa ttaatatact
    13801 gaataaaatc aatctggagg gataattatg aagtgtacaa actttacttt caaaggagag
    13861 gaaggtttag atatatatac atataaatgg gaagatgaaa atataaaaaa accaaaggca
    13921 gttatccaaa ttgcacatgg aatggctgaa actgcacaaa ggtatgagac ttttgccaag
    13981 gtacttacta aaaatggtta tatagtatat atcaatgacc atagaggtca tggaaaaact
    14041 gcaaagataa tagaaaatgt tggacattta gctgaaaaag aaggatttag atgtcttgta
    14101 gaggacatgt atactttaac aaatattata aagaaagaaa atgaagattt gccaatttat
    14161 ctatttggac acagcatggg ttcatttgca agtcaaagat atataatgga ttacagtaat
    14221 aatttatctg gtcttatact atgtggttca aatggaaagc aaggaatcat tctaaatctt
    14281 gctcatctga taatcaatcg tgagattaaa aagtatggaa gacgttctaa aagtaataaa
    14341 ataaataatt tgatatttgg tggcgaaata ataagaagaa atgaaaagac taagtttgac
    14401 tggttaagta gagataaaga acaagttgaa aaatatataa atgacccatt ttgtggagta
    14461 gtgtgtagtt gtggcttttt ttatgattta gttcaagggt taaaagaaat tgaggataaa
    14521 gaaaacttaa aaaaagtgcc acttgatata cctatttaca taatatctgg agataaagac
    14581 cccataggaa aaaatggtaa aggagtctta agactaaggg atagatatat aaaattagga
    14641 gtaaaagatg taacttgcaa actttacaaa gatggaaggc atgaattgtt gaatgaaata
    14701 aatagagaag aagtttttga ggatataatt tgttggttaa ataataaaat agaaatattg
    14761 taataaaaaa atataatgat atatttgctt taaatttatt tatatataat atttgaaaag
    14821 tggattatgt atatgcaaag tagaaaagag agagcccaga agtgattttt tgctataatg
    14881 ccatttattg aataggtggg tgttattata aataaattat taacactcag tattattatc
    14941 attgtatatg gaatttattt tatttaaaat tccttgaaag tttaaatata gtaagttgta
    15001 gtaaagagaa aatataaata tttaaatgct atttaatgtg aatctattaa ttaggttata
    15061 cttaaactta attaatagat tttatatttc aagacaatga gatagggttt tagattaaaa
    15121 tataaccaat ggttaaatta cagacatttt gttaactttc cctcgattta ctagtaaata
    15181 ttttttttat atacttaaat tacaacagat gtactaggga ggtatatatg caaattaaaa
    15241 taggtaaagt catacagcgt cttagaaaag aacgaaattt aactcaagaa caattagcaa
    15301 agtttatagg tgtgtcaaca ccagcagtat ctaagtggga gagtggaaat tcttacccag
    15361 atatagaatt attgccttta ttagcagact tttttaatgt gtctattgat aagcttttaa
    15421 attacaagat tgatttaagt gaagaagaag taatgaaaat ttataaagaa ttagaatcag
    15481 gctttgctag aattgaaatt gatttatcaa cagaagaacc aaaagaagaa tttagacaag
    15541 acttagaatc tgtaaaaaag ctttccaata tgtatataga gaagtatcct aaaagctatt
    15601 tattgaagct aaggatatgt tcgctgtatc agatgtattc atataaattt ggtaagagtg
    15661 aacttaatga cagagtaaaa gaaacaacga atattcttga agatatagtg agaaatacag
    15721 atgatatcca aataaaagaa acagctctta ttatattatc aaacgcatat tgtatgttgg
    15781 aggactatga aaaggcagag ttgtatttaa atatgataca taagtcaata ggggatacaa
    15841 gtgttaattt agctatgata tatttaaagc aaaatagact tgaagaagca gaaatattgc
    15901 ttcaaaataa attgtttagt aatgtattta atataagcat ggattgtaag ggaataatta
    15961 atgtatacaa aaatcaatac aaggaattaa agaaaaaatt ggagaataga aattttaata
    16021 aaaatgcaat tgagagagaa atggagtata taaaaaataa gttgctgggg tatgcaaatc
    16081 tttctttaga aataaaaaaa atgctcagtg aagataaagg tgcatttttt agtatgtata
    16141 tggattatat ggaattatct ctaatttttt tattttttaa catgaaagaa gaagcaaaaa
    16201 aagccttata tagtctaaaa gaaatattag aaaaatatcc tatacatgaa agtttggatg
    16261 taagtcaaat gagatttttt gataaggtag agtctaaaaa tttatatact tttaatatat
    16321 atacaaactt gttaattgta cttaatgatg atagttataa tgagcttaga gaagaaccaa
    16381 tttttaagga tgtaattgaa aagattttag atatggaaaa aatgttgaaa aataaagaat
    16441 aagtaaaaat ttaatatagg atttaaagat taatcaaatt aaaagaaagt gtttcaaaat
    16501 aaaaatttga attacctttt ttgtcggtct taaacagtta tttagaaaaa tagaggtttc
    16561 aaaatagaaa tctgaattat ctgtattatt acacaaaaat agaaacctaa atttataaaa
    16621 atagaataaa gtttgttata taaaatgtaa ttttgtctat aaaatctcac ctgttttctt
    16681 aactttaagc gtgaaaaaga ggatgtatca tgaatttttt agttcatgat gcatccttct
    16741 ttgtatgaaa tccaatatat ctttattatt taaacaatgg aaaagagtct tatttctatt
    16801 ttgagacatc tcttttattt aattatgaag ttttatacag ttcaataaat aggaattata
    16861 tttagaaact catgacatta tttcttagat agtgattaaa tttatcatat tccattttag
    16921 ataacttttc tgggtaagac attacaatca ggttatgccc attaaatttt tcatggtatg
    16981 gtggatgaat gtgttcataa ttatttgtct tatattttat tctttcatag aacgccttgc
    17041 gataaattga aatctcatca attggaaggt caatttctaa aagaatagtt tttcctttct
    17101 taatttaaaa gctctaacgc tctttggcca tatctattat tgcgcatttc tggaaaaatg
    17161 cagaaatgtt ctacatagat aaagtcatct gtttcccaac ataaaattat gccgacaaac
    17221 tggtttttat cataaattaa attgaactga tattctttat ttttcaaaat ttcttcttgc
    17281 aaagaagatt ttctttgttc atgaaatggg aaactaattt tatataattc catagccttg
    17341 taatataatt tattttcact ggttcttaac cgttcaaatt tcactttact cctcctaatc
    17401 actaaaattc caatttattt atgagttcat tttaccatag tcagtatttg catacaatgt
    17461 gttttaagtt gagtgtcatt tctttctttt atttatgttt atatctcata gactaagaga
    17521 taattgctta agacataact aaaaactatt atattaagct ttgataatct aaaaacagta
    17581 tatttatgga cttttctagg tctaaaagta atcttaataa ttttagaagt tacaaaaaag
    17641 ttacataaat ttggaaagct ataatataca tatataatat aaggttaaaa ctgtaatgaa
    17701 taatattaat ttaaaatttg aacatggaag gagattatta tggagaaaag agaaaagagg
    17761 ataataatat catctttatt aagtgtatca attttaatgg gtttggtaag tatatattct
    17821 atattaaata aagaagacat aattttaact gttaagggtc aagagcaaaa agtatcttcc
    17881 tttaagaaaa cagttgagga acttttggat gaacagggta taaagtataa ttctgaagat
    17941 aagattaatc caagtttaga tacagaacta aaagatgata tgaaaataaa agttgttaag
    18001 gtaactaaaa gtaaaaaaga agagattgaa aaaattccat ttgatacaaa gcatgtaaat
    18061 gatagtaatt tgttaaaagg aaaatctaaa gtttatcaag aaggtcaaga aggagaaaaa
    18121 aaactagtct ataatttaac ttaccatgat ggaaagttag tcaaaaaagt cttatcaaaa
    18181 gaagcaatat ctaaggagcc aactacaaaa attataaaat atggaactaa agaaaaagta
    18241 ctaatagcat caagaggagc aaatataaga ggaggcaaac atatgaaagt ggttgccact
    18301 gcatatgcag gagatacaat aacatctact ggtacaactc caagatgggg tgttattgca
    18361 gttgacccac gtgtaatacc atatggaaca aaagtatata tacctaaact gggtatgact
    18421 tttgtagcag aagattgtgg aggtgcaatt aaaggcaata gaatagacat ttttatgaac
    18481 agtgaaggaa aagcttcaaa ttggggaagg aaaagtatag atatatatct acattaaatt
    18541 agtgaataga ataactttac ttgaaggaca aaaaagtata aaatatgaat taactaaact
    18601 gttagattat tcttaaaaca taaaaatata tctaaggggg gataagatga aaatagtctg
    18661 cttaggagac agtttaacgt atggatttgg agtatcacga agtaattcat ggacaaacat
    18721 tgtaaataaa gagactcgat tagaaatagt aaataagggt ataaatggtg atacaacaag
    18781 tggtatgtta gctaggttta atgaagatgt tgtaaaaaat tctcctgata tagtttttat
    18841 aatgggtggc accaatgatt ttattgctgg tgcgggaaat gaaattatta attctaatat
    18901 aatggctatg gtgcatcagt catattcaaa aaatattata cctataatag gtataccatt
    18961 aaaaccagat attccaaatg ttagggaaga ttggagttgt tttactgatt ttaatatagt
    19021 atctcaaaac ttagaatcat atagttattg gataaaaaaa ttttgtatga catttaatac
    19081 taatttcgta gatttttatt cagaatataa taaaaatatg gaactagaag gatataaaaa
    19141 attatatttt gatgggttac atccaaccaa agagggtcat agaataatgg cagacatttt
    19201 tattaattca attaataagt atatagaaga gttatagatt acattaaaat gcttgtttat
    19261 gcgtcagttg caataaatac atacagtaga gaggggttta ttatggatta tactaaaact
    19321 tatgaagagt ggataaaggg ttcatatttt gatgaagata caaagctaga gttggagaat
    19381 ataaaaaata atgaaaaaga aatagaggat agattctata aggatttaga atttgggact
    19441 gcgggtctta gaggaataat tgaagcagga acgaatagaa taaataagta cacagttaga
    19501 agagctactt ttggattggc aaactatata ttagaaaaca caacaaaaga agaaacaagt
    19561 agaggagtag taatagctca tgataataga cacaagtcta gacaattctg tatagaatct
    19621 gcaaatactt tggcagcatg tggtataaaa gcatatatat ttgatagttt gagaactact
    19681 ccagaattat cttttgctgt tagaagctta aatgcaatcg ctggtatagt cataacagca
    19741 agccataatc ctccagaata taatggatac aaggtatact gggaagatgg agcacaagtt
    19801 atgccagaga ttgcaaatgc aataacagaa aaagtaaata gtatacatga ttatagtaca
    19861 ataccaacct taacggaagc taataaaaat ttggtagttt tgttggacga atcacaagat
    19921 actaaattta tagaagcagt aaaaagccaa attataagaa aagatttagt taaaaatgta
    19981 ggtaaaagct ttaagatagt ttatacacct ctttgtggaa cagggaatgt tccaataaga
    20041 agagcattga aagaagttgg ttttgaaaat ataattgtag ttccagaaga agaaaatcca
    20101 gacccaaatt ttgcagggct tgattatcca aatccagaag agaaaaaagc tttaaataga
    20161 ggtatattac tcgcaaaaga aaaaggagca gaccttgtaa tagcaactga cccagattgt
    20221 gatagagtag gtgtagcagt aaaaactact acaggtgagt atgctttact tacaggaaat
    20281 caaattggtg gtatgctgac acattatata atagaaggat taaaagaaaa taataaatta
    20341 aaagaaaatc ctacaataat aaaaaccata gttacatcag aatttggagc agatatagct
    20401 aaagcaaata atgttgatgt attaaatgtt cttacaggtt ttaaatttat tggtgaaaaa
    20461 ataaaattat ttgaacaaaa taaaaataga agttatgtat ttggatatga agaaagttat
    20521 ggttacttag ttgggactca tgctagagat aaagatggtg tagtttcttc attgttaata
    20581 tctgaaatgg cagcatttta ctattcaaaa ggtatgagtt tatatgatgg tttaatagaa
    20641 ctttataaaa agtatggatt tttcaaagaa cagactatat ctttgacttt aaaaggtata
    20701 gaaggtgtag aaaagataaa agaaataata tcttacttta gagaaaatca aattgattat
    20761 ataaatagta ttaaagtagt tgataaaaaa gattataaaa atggtataga taatcttcct
    20821 aaatctgatg ttttaaaata ctttttggaa gatgaatctt gggtagctat aagaccatct
    20881 ggaacagaac caaaacttaa attttacata gctgtaaaag gagctagtga tattgaagca
    20941 gataaaaaga ttcaaggttt gaaaaagtat atagatgaca tggtcgaaaa attaaaataa
    21001 aatattatta ttttataaga taattttgta ataatattta tttagctgaa aattataatt
    21061 tactttcatt gagtagaata tggaaatgtt agtagaattg ttattttcag cttttatttg
    21121 tgttattttt acttaaataa ttatattata gaatgaatct gagtataatt aatattgtat
    21181 aatgaaatta atttgataga aaaggggata tttatgtttg gtatagataa gaaattgatt
    21241 gaatatataa ttgcaaaatc caattacagt gaattaagtt caaagtcagc agaagtatct
    21301 ttcttcttaa tgttgtccat atttcctttt ttaatattta caataagttc tatagcttat
    21361 ataccgattc ttcatttaaa taagtatata gctttgttta gaaatatgat gccagaaggt
    21421 gcttttgctg tgttatcttc aatcatagtt tctgcaatag acaatagaaa cctgaaattt
    21481 ttagcagtca gttttgtact tactatgtgg acattttcaa gagcagtaaa agctcttatt
    21541 aaaggaatga atagagccta taaagttaaa gaaactaggt ctttttttaa aattttatct
    21601 atatcatttt tatttacaat aatgcttttg gtccttattt ttttatctat gatattttta
    21661 gtttacgggg aaaaaatagg ttattttatt tttaatcttg taggattgga tgagatattt
    21721 ataaaaatat gggatattct aagatacact gtaggtataa taacaataat agtgatattt
    21781 acacttttat ataaatacac tccaaataaa aagttaacta ttaaagaatc tgcgccaggt
    21841 gctatttttg ctacttttgc ttggttttta gtatcatttt tatattctta ttatacaaat
    21901 tactatgcta actatgaagt aatctatgga agtatagctg aaataattgt acttatgacc
    21961 tggatgtatt ttagtagttg gtctatcgtt attggctatg aggtaaattc aagattgtat
    22021 tttagaaaga taagacatga aatgttaaag taaagtattt aaaataaaag agagtattta
    22081 gtattataaa tgtaattgaa ttaataaaga aatattgaca tttaagttaa aaagtctaga
    22141 atttttctgg gcttttttta tttttagtat aacttg
//
LOCUS       DAEMHW010000050        21782 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEMHW010000050 DAEMHW010000000
VERSION     DAEMHW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.050"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..996
                     /locus_tag="KPK67_001631"
     CDS             <1..996
                     /locus_tag="KPK67_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2458617.1"
                     /translation="RLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPEDRDINSIIDR
                     ANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIYLQPKIELNT
                     GEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIYLRKWIDAGI
                     NPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDNFDILIDIMN
                     NLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSKIIVTKVIEM
                     AKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIVSNLVRG"
     gene            1172..1540
                     /locus_tag="KPK67_001632"
     CDS             1172..1540
                     /locus_tag="KPK67_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2458618.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            1542..2267
                     /locus_tag="KPK67_001633"
     CDS             1542..2267
                     /locus_tag="KPK67_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2458619.1"
                     /translation="MDKNINNVSQGGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            2261..3025
                     /locus_tag="KPK67_001634"
     CDS             2261..3025
                     /locus_tag="KPK67_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2458620.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLTLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            3247..4497
                     /locus_tag="KPK67_001635"
     CDS             3247..4497
                     /locus_tag="KPK67_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2458621.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYEFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            4688..5572
                     /locus_tag="KPK67_001636"
     CDS             4688..5572
                     /locus_tag="KPK67_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG2458622.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLVLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            5934..6551
                     /locus_tag="KPK67_001637"
     CDS             5934..6551
                     /locus_tag="KPK67_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG2458623.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTTDSGQAQDNTKKEANAPN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIIKPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            6659..9190
                     /locus_tag="KPK67_001638"
     CDS             6659..9190
                     /locus_tag="KPK67_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2458624.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGNKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            9624..10325
                     /locus_tag="KPK67_001639"
     CDS             9624..10325
                     /locus_tag="KPK67_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG2458625.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            10375..11457
                     /locus_tag="KPK67_001640"
     CDS             10375..11457
                     /locus_tag="KPK67_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2458626.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLVIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            11718..12818
                     /gene="vanG"
                     /locus_tag="KPK67_001641"
     CDS             11718..12818
                     /gene="vanG"
                     /locus_tag="KPK67_001641"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG2458627.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            12815..13621
                     /locus_tag="KPK67_001642"
     CDS             12815..13621
                     /locus_tag="KPK67_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2458628.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVYTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            13639..15777
                     /gene="vanT"
                     /locus_tag="KPK67_001643"
     CDS             13639..15777
                     /gene="vanT"
                     /locus_tag="KPK67_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG2458629.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLIITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGIFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKDSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            16091..16711
                     /locus_tag="KPK67_001644"
     CDS             16091..16711
                     /locus_tag="KPK67_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG2458630.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(16877..17413)
                     /locus_tag="KPK67_001645"
     CDS             complement(16877..17413)
                     /locus_tag="KPK67_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG2458631.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(17526..18200)
                     /locus_tag="KPK67_001646"
     CDS             complement(17526..18200)
                     /locus_tag="KPK67_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG2458632.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            18403..20277
                     /gene="asnB"
                     /locus_tag="KPK67_001647"
     CDS             18403..20277
                     /gene="asnB"
                     /locus_tag="KPK67_001647"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG2458633.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(20369..>21782)
                     /locus_tag="KPK67_001648"
     CDS             complement(20369..>21782)
                     /locus_tag="KPK67_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG2458634.1"
                     /translation="KISIKKSTEPLIEFISDTTYKNKHSSYIKEVYFTRYIGVDDSID
                     KYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSKFNSWDLYN
                     IDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWMDYFLPKLF
                     YNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYPVCSKFSTL
                     FELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLTSKKIAQDE
                     EVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVVNDGYSFLV
                     RFENDNKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIVAIGSPLGL
                     FNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAGYDDGQNLN
                     LAVESSLVKIFANNFIEIVN"
BASE COUNT         8295 a         2287 c         3700 g         7500 t
ORIGIN      
        1 aggttaaaaa ctatatttga agaattagac ttgtttaata ataatcaaga agaaaaatat
       61 aagttagttt taagttgtgg aatctatttt atacttccag aagatagaga tatcaactca
      121 atcatagata gggctaatat acctcataaa atggcaaagg gaggacataa aagttcatat
      181 gcattttatg ataataagat tcatgaccaa gagataaaag aaaaagagat ggaaaataca
      241 atgttttcaa gtctagaaaa taaagaattt attatatact tacaacctaa gatagaactt
      301 aatacaggag agatacaagg ttcagaagca cttgttaggt ggaaacgtcc tgataaaggg
      361 ctaatacctc caaatgaatt tatcccattt tttgaaagaa acggttttgt tataaattta
      421 gatttatatg tgcttgaaga agtatgtata taccttagaa aatggattga tgcaggaata
      481 aatccagtta ctgtttcagt taatgtttca aggatacatt tatattgcaa taattttata
      541 gaaacataca aaaatataat tgataaatac aatatacctg caaagtatat agaacttgaa
      601 ttaacggaga gtatcatatt tgataatttt gatattttaa ttgatataat gaataattta
      661 aaaaaaatag gatttttaat atctatggat gattttggtt cagggtactc atcattaaat
      721 atgttaaaag aaattcctat ggatatctta aaacttgacc aaaaatttat tatggaaact
      781 tataatagta aaagaagcaa aataattgtt actaaagtaa tagaaatggc aaaagagttg
      841 ggtatgaaag tcatatcaga aggagttgag acagaagaac agtttaaact tttaaaagaa
      901 gtaaagtgtg atatggcaca aggatatttg tttggaaagc caatgccaat tgaagaattt
      961 gaacatttaa ttgtatccaa cttggtaaga gggtaattaa tttgtaatat aaagaattta
     1021 ataaattgtg ttaaaaatgg actctaatgt agtgttcatt ttttttgtaa aattttttat
     1081 acttttacta aaaagtaatt gacatatact ttgtattatt atattattgt attaacaact
     1141 taatacaata atataataag gaggcatgaa catggaatgg gaacttgata ataacaaacc
     1201 tatatatata caattagtag aacatttaaa gttaaaaata atttcagggg aaattaaaat
     1261 aggatctaaa ttagaaacag tgagagcact agcagaagat gcggaagtta atccaaatac
     1321 aatgcaaaag gctctaacag aacttgaaag acaaggttta gtttatagtc agagaactaa
     1381 aggaagattt gtaacagatg ataaggaaaa aataaaagct atgaaagagg aaatagctaa
     1441 tgtggagata aatacattaa aagtaacctt agaaaagctt ggatatgata gagatgaaat
     1501 gctaaaactt attacagaaa atctgaaagg agaattataa aatggataaa aatataaaca
     1561 atgtttctca aggtggaata gttgaattta aaggtgtaaa caagagttat ggaactaaga
     1621 atgttttaaa aaatattgat ttaaatatac caaagggaaa aatagtcgga ctgttaggac
     1681 ctaatggaag tggtaaaagt acaatgatta agctaatgaa tggattatta cagcctgata
     1741 atggtgaaat tatgataaat ggaatgaaac catccataga tactaaaaaa atagtttcat
     1801 atttaccaga aagaacttat ttaaatgatt ggatgaaggt atcagattta cttaagtttt
     1861 tctatgattt ttatagtgat tttgatgtta gaagagcaaa tgagatgatt aaaagcttag
     1921 atattgatgt aaatgaaaag ctaaagacaa tgtctaaagg aactaaagaa aaagttcaac
     1981 ttattttagt aatgtcaaga aatgcgagca tatatatatt ggatgaacct ataggtggtg
     2041 ttgacccagc tgcaaggtca tatatactta agactatatt aaaaaattat tctgaagata
     2101 gtactttact gattgctacc cacttaataa gtgaaataga aaacatatgt gatgagataa
     2161 tattcatatc aaaaggagaa atagtgttac aaggagacgt tgaagcaata agagaagaaa
     2221 aaggaaaatc tatagatgca ttatttaggg aggaatttaa atgttaggta aattattaaa
     2281 atatgagtta aaagcaagtg gaagaatatt tataccgtta tatatagcca tattgatagt
     2341 agctgtattt aatggaatat ttatgaatac aaatatactt caggtacagg gaataggaat
     2401 actagtatta acaagtcttt ttatggcatt aggagtttta actattgtag ttacaattca
     2461 aaggtttaga aaaaatcttt tgggggatga aggttattta atgtttactc ttcctgttag
     2521 cactagttca cttatattat ctaaatgtat aacagcactt atatacgctg tacttagttt
     2581 tatagttgca gtattcactt ttggagttct tacgcttttt ggtacttctg gaattttact
     2641 acctgaaatt ttagatttgt ttaacactag ttttaaatgg atttctgaaa actttttaga
     2701 tattttatta ttagtagtag taatgtttat ttcatattca tcatttattt tgttattata
     2761 tacatcaatc tcaatgggac aattaccaaa attcaataaa catagaaaca tagtggcatt
     2821 tgcttctttt atagctataa atatagtaat atcaattgtt ggagatgcag ttggaagcat
     2881 cttaccaaat gaagatacaa atatggtata tcatctttat cagagtccta gttttatgct
     2941 tgcaatactt ggtagtcttg tagttgcaat agcgttattt ttcgctacaa aatttatatt
     3001 agataaaaaa ttaaatttag aataaatgtt tttatatgga gctatctttt gagtaatgtt
     3061 aaaagatagc tcttattatt tatgtagtta attcatgtat ataatatgaa tagtgtttta
     3121 tttctagtta aatatataca tatgttgtat aatgatattg cgtgtaaaaa tttgtaagaa
     3181 taactataca ctttatataa gttatatcaa gaaaatttaa aaatatatgg atttgaggga
     3241 gaagatatga aaagttatac aaaggctaag tggagtgttt ggggtataat tacattttca
     3301 tttgttttag tattattttt aaggatgtca actgctgttg tttcagacaa tttagcaaat
     3361 gagttaggtt ttaattctat acaaatttca aatatagcat ccttttgcct atatgcatat
     3421 gcatttatgc aaatacctgc aggaattttg attgataaat atggagcaag aaaaattagc
     3481 agtcttggaa taataatggc aagtttaggg tcaattttat ttggtttgat tcaaagtata
     3541 gaattagcat atatttctcg tgtaattgta ggggcaggaa catcagtaat attactttgt
     3601 attttaaaaa ttcaagggag atggtttaat aagtcagaat ttgcttctgc aacagctaaa
     3661 ttttcatttg taggaaattt aggtggagtt cttgctactt ttccacttgt gtttttgtct
     3721 gaacttgttg gttggagaaa ttcattttta ttaatcggta taattggtgt tgtaattgga
     3781 tgttttatgt atattattgt tagagataca ccaaaagagt atggattcaa tgtagatact
     3841 gaaccctatg aaaagtcaga gaaggttaat attgttgatg gtattaagtc tgttataaag
     3901 aacaaatcaa catggtataa ttcaatgata atgttctctt ttgttggtct tacaagtgca
     3961 tttataagct tgtggggagt aagatacata atggacgttt atggtgtgag taagagtttt
     4021 tctgcgttta tagtatcatt ttttacatat gaatttatat ttggttcaat aattatggat
     4081 tttgtatttg ctaaaataag gtctagcaaa tttaatataa taaaatttgg agcaatgata
     4141 gatttgttta tatggattgt aattgtagtt gtataccagg taaaaccacc aattatagtt
     4201 ttaccaatat cattctttat tatgggatgt atagttatgt cacatctaca ggtttttaat
     4261 gatgccaagt ataaaaataa agaaatttat tctggcctgg caactagtgt tattaatact
     4321 tttgaattta taggaagtgg aattattaat ttgattatag ctatctcttt acaagtaaat
     4381 tcatataata tagttgatgg atataaaaaa ggttttgtag tatttatagt actaagtatt
     4441 ataactatag tatcatctca tataggggta aaaaatgatg attttaaggc tatatagtgt
     4501 tttattaata gtttaattta gtattatatt ttgcatatat ataacaaata gtttgcattt
     4561 tcctaagatg tataattgga atataaatct tgtttactat gtacaaatgg tattgtatta
     4621 tattaaatag aaaataagaa ctttaaacta taatattggt tctaaaatat aaggtaagga
     4681 gttttttatg gatacgacga aaatagatga tataagcaag ttagatttaa ataatttaga
     4741 cattaataag atggatatgg gaggtataat ggaaagtctg ctagaatggg ctactacaag
     4801 cggagtaaaa cttataatag gtatacttat actttctatt ggatttaaaa taataaaaaa
     4861 atttgtaaac catgttatgt tagtacttag taaaagagat attgatttaa ctttaagaag
     4921 gtttttgaag tctcttcttt tgagtgtact taaagttgcc gttataatca ttgttctgga
     4981 atattgggga atgagtttgt ccagttttgc tgctgtgata gcatcagcag gggtagcaat
     5041 aggtttggct ttacaaggga gtctatcaaa ttttgctgga ggatttatta tactattgat
     5101 tagacctttt aaagtaggag attacgtaga agcagcaggc catggaggaa cggttgaaca
     5161 aataggactt ttttatacac aattggttac tccagataat aaacagatac ttatacctaa
     5221 tggttctgta tcaaatgata gtctaataaa ctattcagca aagaatacta gacgagttga
     5281 tttgactttt agtgtagggt atgaagatga tatactacat gttagaagag tacttaaaga
     5341 tattgttaat aatcataaat taataataaa tgaaccagaa ccatttatag gagtagtaga
     5401 acatggagat aatgctatta aatttgcaac tagagtttgg tgcaagacag aagattattg
     5461 gacaatttat cttgatttac tagaagaggt taaagttagg tttgacgaag aaggaattac
     5521 gattccatat cctaaaatgg atttaacggt aaaagaatta aacaaaattt aattttttaa
     5581 tatagtgttt aaatttaaga atatcatata gatatactta taaggtttgt aaaaaattaa
     5641 taagtactgt tattttgtaa gtaaaaatat aaaaaaatgg gagtgtctca aaatgaactt
     5701 tttagttcat gaggcactct ttttgtacga aataaaatat atttttatta tttcaacaat
     5761 gaaaaagatg cttgtttcca ttttgagaca gtataatttt tatgtaactt taattttagt
     5821 aaatataaag ttttaaaatt gataaaatag gaggaatttt taatataaaa gggtaaataa
     5881 agggtttaga gggcataata tagaataaat taatatattt aaggagggtt tttatgtgta
     5941 aaaaaacgta tttattagtg gtaagtatgc ttatagcggg tttattaact gcatgtagtg
     6001 ggagtccaac tacggattca ggacaagcac aagataatac aaaaaaggag gcaaatgcac
     6061 caaataacgc tttaaaagat gaaaaaaata atgaaaattt aatggaacaa gattttaaag
     6121 tgccatacac agatgcaata aacatattta aagataaata taaggatgct gatatagtag
     6181 atttaagtct tgagagagat ttaaataaat ttgtttatac tgtggaaggt gtagatgata
     6241 ataatgaata taagatgaaa attgatgcta atacaaaaga tgtattagaa gataaaacag
     6301 aaaagttgga ttcagaagat ttaaatggag ttgctagaaa agaaaaatta gatttaaatg
     6361 acataattaa acctcaacaa gctatggaaa ttgcattaaa agaacaaaat ggaatggtta
     6421 aagaatggag tttagacaaa gatttagatg ttacttttta taaaataaga atagataaag
     6481 ataaaaatga atatgacata aaagtagatt ctaaaaaggg aactgtattg aaggtagaaa
     6541 aagaagatta gaagatttaa actatcttta aaataatttc ttttttgaat aaattttgtt
     6601 taaaatataa caagcagggt ataaagagac taaataacta ttttaggagg agtacggtat
     6661 gtcattgaaa tcactaaaaa taaaagaaaa tttatattgg gtaggttcac ttgaccctga
     6721 tttaagagta tttgacataa ttatgtatac accttatgga actacttata attcatatgt
     6781 attaaaagga acagaaaaaa cagttttatt tgagacagtt aaagataagc attttgacaa
     6841 ttatattgaa agattaaatg atttaaatat agattttgaa aaaatagact atattgttgt
     6901 aagtcatact gaaccagacc atgcaggaag tgttgaaaag cttttggatt tagctaaaaa
     6961 tgcaaaagta gtagcttcag aaacagcaat taaatatctt aaggaaatcg taaacaaaga
     7021 ctttgaatat gttgcagtta cagatggaga tacattatca attgggaaca aaactttaga
     7081 atttttctct gtacctatgc ttcattggcc agatactata tacacatata taaaagagga
     7141 taaaactctt gtaacatgtg attcatttgg tagtcactat agtaatgata agatagtaaa
     7201 tacacttgat gaaaatgaag aaaaggatta cttggatgct ttaagatact attatgattg
     7261 tataatgggt ccatttaaac catctatggt aactgctata gaaaagataa aagatttaga
     7321 tatagatact gtatgtccag gtcatggtcc agtattaact gaaaatccta gaaaaattat
     7381 agacctttat tacaactgga gtgtaaatga acaaataaag ttagaaaaag aagttacaat
     7441 ttgttatgtt tctgcacatg gatatacaaa gattatggca gaagctataa aagcatatat
     7501 tgaaaaaaat agcaactata aagtaaattt atttgatgta atagagcata agcaagaaga
     7561 tattttggca aaaatagcag tatcacaagg tgtattattt ggaacaccaa caatattagg
     7621 agatgcgtta aagcctatat gggatatatt aatatcgtta aacccagttc ttcatggtgg
     7681 aaaagttgct tcagtatttg gttcatatgg atggagtgga gaaggtatag aaaatgctat
     7741 ggagagaata agtcagctta gaatgacagc agtaaaacct tttgctgtta actttaaacc
     7801 atcaaatgaa gagattgata aattacattc ttatacaggt aaattcctag ataagttaaa
     7861 ttctactttt ggaagtaaaa agaaaactaa gaaatttaaa tgtgtaattt gtaatgaagt
     7921 ttttgaaggt gatagtgctc ctagtgtatg ccctgtatgt ggagctaagg aagaccaatt
     7981 tatagaagtt gaagaagatg aagttacttt tagaaaagat actgatgaat atttcgtaat
     8041 agtaggaaat ggagcagctg gtttttatgc agcagatgct ataagaaaaa gaaataaaac
     8101 ttgtaagata actatgattt ctaatgaaga tgagttgact tactatagac ctgcactttc
     8161 tgatggtata aatgaagaac ttggttcaga tttctacatg gaagataaag attggtatga
     8221 taaaaataat atagtagtaa tattaggaac gaatgtagat aaattagatg aagtgaataa
     8281 aactattata gtgaatgatg gagctattaa atttgataag cttgtaatag ctacaggaag
     8341 tagaaacttt ataccaccaa ttaagggtca tgatttagaa aatgtgttta ctcttagaaa
     8401 tataaaagat ttatatagtg taaaagaagc actagaaaaa tctaagaaag ttgtagtgat
     8461 tggtggcggt ttattgggac ttgaagctgc atgggaattt agactaaaag gattagaagt
     8521 agtagtagtt gaagccatgg atagcatact atcaaaacaa cttgataaag aaggtagtaa
     8581 aatattagaa caatgtgtaa gagatacagg aatagatgta agattgggtg tagcagttga
     8641 tggtattgaa ggtgatggaa aagctcaaaa agttgtattt aaagatggag atagtgttga
     8701 ctgtgatatg gtagtatttt caattggtgt aagagcaaac actcaaatgg ttcaggatac
     8761 atctgtaaaa attgatagag gtattgttgt agataagaca ttacagacaa atgttaaaga
     8821 tatatatgct tgtggagatg ttgcacaagt gggaaatatt agtttagcaa tatggccatc
     8881 ttcagtagaa atgggaaaaa tagcaggggc aaatgcaagt ggagataatt tgacatttga
     8941 aagtgaagta tatccagttt cattagatgc aatgaatgta aaagtattta gtattggtaa
     9001 tatacaaaat tttgataaag aaatttcatc aaaagatgaa ggtcaaagaa tatataaaaa
     9061 attatttatg aaagatggtt cattagttgg agctatactt ataaatgatt taagctgtac
     9121 tgttaaattg ataagattaa tatctgaaaa aggtgatttt gaggatatta tgaaggctga
     9181 tatactataa tttaaataca ttaaaataaa aaggagttgt ctcaaaattg atttttttct
     9241 aatcaaattt taaattcaac tccttttttg aatttttata taaaataaca tggaaatttt
     9301 agtaatgtct gtggttacat acataggttt aattgttttt tatatggatt gtctctggta
     9361 taataaattg aattgtgttt gactgataga tatttttatt gaaagttaat acctgttatg
     9421 cgataataag gacgtgttag gggagttttt ataataacaa ttaatctcag tttttgtgtt
     9481 ccaatagtag catcaacaca ttctgatgta gtagttcttt tcttataaaa attagttaca
     9541 aatatgcttg taattatttg atatgtaaat ttaccaatat tataaatgat gacctcgata
     9601 ataaggagag tgaaaaataa tctatgaacg aaaatatttt gattgtggat gatgaaaaag
     9661 aaatagcaga tttaattgaa gtatatttaa aaaatgaagg ttttacagta tacaagtttt
     9721 atacaggtaa ggaagctcta gaatgtattg aatctaaaaa tttggatatg gctattttag
     9781 atattatgct tcctgatatt gatggatttc atatttgcca gaaaattcga gaaaaatact
     9841 attatcctat cattatgtta acagctaaaa ttgaagatat agacaagata atgggtttaa
     9901 ccataggtgc tgatgattat ataacaaagc catttaaccc tttggaagtt gttgcaagag
     9961 taaagacaca attaagaaga tatatgcgat ataataattc ttatgaacaa cagtcgataa
    10021 ttgttaatga gtatgatatt aaaggattga ttatcaataa agaaacacat aaatgtagtt
    10081 tatttgggaa agaagtagca ttaacaccga ttgaattttc tattctttgg tatttatgtg
    10141 aacatcaagg aaaagtggtt ccttctgaag aattgtttga agctgtatgg ggagaaaaat
    10201 atcttgataa taataacaca gttatggctc atatagggcg attacgtgaa aaaattcatg
    10261 aaccttctaa aaacccgaaa tttataaaaa ctgtatgggg agtaggttat acaattgaaa
    10321 agtaaaaata ttaaagtcaa tcctgacttt actcaagtga gaaataaaat ttttatgaga
    10381 acagctttaa tggcatttat agccattatt ttcatatgga ttttatattc ttttatattg
    10441 tatggtaagt tttctatctg gatagtatca ttctttgaaa atgcttttcg tatggaatat
    10501 aaagctgcat tgaatttata tcagcagatt ttccgtaata gcatggagct cttcatgata
    10561 ctagctatgg tgattgtgtt ttttataata ttccgtgttt atttaaattg gtttacaaaa
    10621 tattttagtg aaataaatca aggtattgac tctcttatca aagaagatgt tggagaagtg
    10681 gctttatctc cagagttatt ggcaatagaa aagaaaataa actcaattaa acatatactt
    10741 gaacaacgta aatttgagac acaaatggca gaacaacgta agaatgaact tattgtttat
    10801 cttgcacatg atttaaaaac accacttaca tcagttatag ggtatctgac actacttcga
    10861 gatgaaagtc agatttctga agaactgcga aaaaaatatt tatctatttc gttggataag
    10921 gcagagcgtc ttgaagattt aattaatgaa ttctttgaaa ttacaaggtt taatttatca
    10981 aatatatcac ttgaatacag tacagtaagc ttaactcgta tgcttgaaca actaacatat
    11041 gagtttaaac ctactttgat gagtaagaat ttaaaatata cactgaatgt tacaccaaat
    11101 atgatgataa ggtgtgatgt taataaaatg caacgtgtat ttgataactt attacgaaat
    11161 gctgtttatt atagttttga agatactact attgaaataa cagctgtaca ggaggatgat
    11221 tatataaagc ttaaatttat aaatcatagc aatacgattc cagaggaaaa acttgagcgt
    11281 atttttgaac aattttacag gttggataca tctcgtaatt caaacagtgg tagagctggg
    11341 ttgggacttg ttatagctaa agaaatagtg gagcttcata acgggacaat aacagctcat
    11401 agcgagaaca atataattac ctttaaggtt attattccga ttgtaggaaa tttgtaagaa
    11461 tttaagtata aaaaaatagg aaattcttta ggataaataa ataaagagca ctcttgttat
    11521 tgatataatt actgtatcaa aacaagagtg cttttatttt gatacagtaa ttatatcaat
    11581 agaaaaatgt gaaaatatat tatgtggaac agatgcaatt gtaagtgaag gttgattgga
    11641 tttgaaatag aaaaagtatt ttaacatgtt tctgtagatt gtataaaaat aaataaatca
    11701 acaaaggaga atcaaagatg aataataaaa aaattgcagt tatatttggt ggaaactcaa
    11761 cagaatatga tgtatcatta caatcagctt ttgcagtact tgagaatttg aatacggaaa
    11821 aatatgatat tataccaatt ggaattacaa gggagggtaa ttggtatcat tatattggag
    11881 attataataa tatccagaat aacacttggt ttgaaaggga tactgatata attccagtta
    11941 ttgtttcgca gaaccgtgtc aaaaatggta ttattgaatt tttaattcct gatgctgttt
    12001 ctcagaatca tattccaaat agtgctattg aatcatgtga aaatgatatt aaatacacta
    12061 aacttgattt tgtatttcct gtattgcatg gaaaaaatgg agaagatgga acagtgcagg
    12121 ggttatttga attggcaggt atacaggtta ttggatgtgg tactctttct tcagcattat
    12181 gtatggacaa agacaaatca cataaacttg ttcattcaga aggtattaaa gtaccaaaat
    12241 caatattact aaaatactca gataaattta atgtggctga actgtcaaaa gacttaactt
    12301 atccattatt tataaaacct gtgcgtgcag gttcatcatt tggtattagt aaaatttata
    12361 aaaaagaaga gttacaagta gcagtagact tggcttttga acatgataat gaagtaataa
    12421 ttgaggaaaa tattgatggt tttgaggtag ggtgtgcagt tttaggaaac aatgacttac
    12481 ttgttggaag agtagatgaa atagaattgt caaaaggatt ttttgattat acagaaaaat
    12541 atacattgaa aagttcaaaa attcatatgc cagctagaat tgatttggat tgtgagaaaa
    12601 gaatacaaga gacagcaaaa attatttata gaactcttgg atgttctgga tttgctagag
    12661 tagatatgtt tttaactcca tctggtgaaa ttatttttaa tgaagtcaat acaattcctg
    12721 gctttacttc tcatagtcgt tatccaaata tgatgaaagg tattggactg tcttttgaag
    12781 aaatattgga caagataatt gaggtgtaca taaaatgatg aataatattt tattagataa
    12841 aaataaagaa gataaaatga caaatactat cttattgact aaagataaag tttatgatgg
    12901 gaatctaatt ctagtaaatg cttttttacc agttaaaaca tctgaagata tagatttaat
    12961 tcctgtatat acaaggtttc caagtattct tatgaaacgt gaagctacaa acattttaca
    13021 aaatatttta aaaagtatat gtggaataaa tgaaatagta cctgtaagtg gatatcgtac
    13081 tgcagaggaa caacaagata tatattcaag ttctctgcgt gacaatggaa aagactttac
    13141 taaaaagttt gtagcacttc caaatcacag tgaacatcag acagggcttg ctattgattt
    13201 agggttaaag aaagaagtta tagattttat ctgccctgaa tttccttata atggtatttg
    13261 taatgagttt cgtaaggcag cactacatta tggttttata gagcgttatc agtgtaaaaa
    13321 agaagaaata acaggaatat ctcaagaacc atggcatttt cgttatgtgg gatatccgca
    13381 ttctgagatt atcaataaaa tgaatctcgc tcttgaagaa tatatagaat atattaagag
    13441 attttcgtat acaaattgtt taaaaataga agataaagaa aaaattatag aaatctttta
    13501 tgtgcctatt catactaaag aagaagtgag aatctctata cctgaacaat gtgtatatca
    13561 aatatcgggt aacaataagg atggttttat tgtgacattt tggagaaata gaaaggacta
    13621 agaaatggag gaacaagaat gattaggaat aaatcttatg cagggcttga ttattttagg
    13681 attatagctg cattacttat aattgcaatt catacatctc cattattatc atatagtaaa
    13741 atggctgact ttgtgttaac tagtataatt gctcgtattg ctgtgccatt cttttttatg
    13801 ctatcaggct tttttctaat ttcaacatat aattataatt gtaataaact gaaatctttt
    13861 ctaaaaaatg tattatttat ttacgggata gctattgtaa tttatattcc cttaaatatt
    13921 tacactggat atttttccat gaataattta gtgcccaata ttattaagga tattgtattt
    13981 gatgggactt tatatcattt atggtatctt cctgcatcaa ttattggtgc aagtattgca
    14041 tactttcttg taaaaaaact aggtttaaag tactcactaa taatcactat tgttatttat
    14101 gtaattggta tgtttggtga tagttattat ggttttttag aaaaaatacc actattaaag
    14161 aatttatatg ggtatatttt tgaagtatca gattatacac gaaatggtat attttttgca
    14221 cctgtatttt tcatacttgg tggaataata tctaataaat ctaattatat gtcaactaaa
    14281 aatagcttga ttggatttct tatatcattc ttacttatgt tatgtgaggg aatattttta
    14341 cataaactag gggtacagcg tcatgatagc atgtatatta tgttattgcc atgtatgtat
    14401 ttcttattta cagccttaac atttttgaaa gattctcgag ctaggttatt gcgaacatca
    14461 tcattaatta tttatattat acatcctatg atgattgttg ttattcgtat gttttctaaa
    14521 atattcggaa tacaaactgt cttggttgac aatagcctta ttcattatat agtggtaagt
    14581 atagcatctt ttatagcttc tataattatt atattgattc ttaaacctct aaaaaaagac
    14641 ttgaaaggag aacataaggt aaataccaat agagcttgga ttgaaattga tttaaataat
    14701 ttggaacata atgtaaagat gatacaaaaa gctatgccag aagattgtga attgatggca
    14761 gttgtaaaag ctaatgccta tggtcatagt gcttttgaag tagcgacttg tgcaaaccaa
    14821 ataggtgtaa aagcatttgc agtagcaaca atagatgagg ggattgattt acgtcgatat
    14881 ggaatactag gagaaatatt gattcttgga tatactaacc caataagggc aaaggaactt
    14941 cataaataca atcttacaca gagtgttatt gaccttaatc atgctatctc tttaaacaaa
    15001 cagaaataca acataaaatc acacattaaa attgatacag gtatgcacag actgggtttt
    15061 gatgttaatg atgtgaggtc aattttaaag gcatttaaat taaaatactt agatatatgt
    15121 ggcatattta cacacttgtg tgtttcagat agtctgaaag atgaagatgt agactttaca
    15181 aataaacaaa ttgaatgttt ttataagtta attgattttc tgttaaaaaa aggtataaaa
    15241 attcctaaag tacatattca aagtagttat ggttttttta attatcccaa tcttaagtgt
    15301 aattatgtaa gaattggaat tgcattatac gggatattaa gttctccaaa tgatacgact
    15361 aaattgcaac ttgatttacg acctgtttta tccttaaaat cacaagtaat tcttattaga
    15421 aaaatacaaa aagatgatag ttttggatat ggaagagtct ttattgcaag cagagatagc
    15481 gtaattgcta tattatctat tggctatgcc gatggtatac ctagaaattt atcctgtgga
    15541 aaaagttacg taataataaa tggttgtaga gcagcaatag tgggtcgaat ttgcatggac
    15601 caacttgctg ttgatattac agatatacct aatgtagaag ttggtaatac ggcaataata
    15661 ataggaagag ataatttatc tgaattatct gcttctgaag ttgcaaataa ctcatgtagt
    15721 atatcgaatg aattattgag cagagtagga agaaggttaa atgttataaa gaagtaactc
    15781 aattaaatag taatatttgt tgtactgtgt aatttatttt actctgacta gacattttta
    15841 gtaatattat gtctaatata tattttttat aacttacatg acaatttaag ttataaaaag
    15901 gtgatgtttc agatttttat tttgaaacat cacctttttt gttataatat ataataaaat
    15961 aattatacct taactgtaaa cttatataga ttatttactt tttaatatta gattcatttt
    16021 ttgagattgg tatataataa acatatagag tatataataa tagttaaagt taaaaaaggg
    16081 gaaaagcaat atgcagtata tacaagaact acttgatttg actcaaaatt attgggtgtt
    16141 agccacaata atcggcttat taagtgcatt tatagaaagc tttataccag ccttaccact
    16201 tgttgctata gttacagcaa atgctgcaat acaaggatta tttattggat gtttgttatc
    16261 atggattggc tcaggacttg ggacaacttc attattctta ttaataagca gatttaatga
    16321 tagtaagctt tttaataagt tgagaaatag taaaactgaa aaagcaataa gttggatgga
    16381 taaacaggga tttaagttat tatttatagc gtatgcctgt ccatttatgc ccggttgttt
    16441 agttactatc gcatcagctt ttggtaaaaa agatttaaaa gattttgtgc cagcaatgtt
    16501 ggcaggaaaa tttgtaatgt ttatagttat aagttacgtg gcaagtgata tagaaggttt
    16561 tataacaagt cctttaaaga tagcatcatt tatattattg gtgtttttgt catggaagat
    16621 tggaaataga gtaaataaaa atcttgaaaa tcataattat gatttccatc ataaaaaaca
    16681 tcacaatgat aaagatgata aaatgattta gcttgtacga aataaaacgt atcttaaaat
    16741 gatttagtac taaattattt taagatacgt tttatttttt gtattaaggt acaaaatttt
    16801 tagttctttt gtaaactaaa atatataaaa cttatttata cttagttcaa aatattgtta
    16861 tttatttttt ctacaactat aataaaatat aaaattgata tccaatcaaa aaaataacta
    16921 tgaaatttaa tagcttagat atgaattttt ctgtttttga gttagttttt ataggtagta
    16981 aagaaatgtt tatggtaatg ggataaaaca attcaatacc ttctttagta ataaggtcaa
    17041 gacacaagtg agatagatat cctattataa acccactgga caaacatgag aaaactatat
    17101 tattgttagt gaagattgtc agagatttaa aaaaactata aataagtaat aaggcaatta
    17161 gactgtgagt aaaccctcta tgtctaaacc tacttccaaa taatttgtat attaacataa
    17221 attttttact tatgtaagaa cctctcatat ctatatctgg aaataaagaa cctaaatatg
    17281 caaaataaat ataaattata attaatacta ttttatatgc aatatcgtat ttaactaggt
    17341 acagattgtt tataaagggg agtatcaaaa gtgcgaatat ataacctccc ctttcatgtg
    17401 tttcttttat catataaaaa tcctcctgaa tcgtcactat aaacattata ttggtaaatt
    17461 tagactatct tttattatat tatatagcaa acataagttt ttttcaaaag gattttaata
    17521 tttaattaat cttgtgattt taaatttttg taattttcta aagcaccatt ccaatttact
    17581 acattaaacc agttgtcaat gtattcattt cttctatttt gatattttaa atagtaagca
    17641 tgctcccaaa catcaagtcc tattatagga gttaggtttt tacttacagg gctatcctga
    17701 tttggagtag tcataataga taatttccca tctttagtag ctacaagcca agcccaacca
    17761 gaaccaaaga catctaaagc agatttttgg aactcttgct taaatttttc aaaagaacca
    17821 aagtctctat caatagcttc ttttaaagat tcagaaggta tggttttttc tggtgtcatt
    17881 atatcaaaaa agaatttatg attataagct ccacctgcat tatttcttac agttgtagca
    17941 atatctttag gtaaagaatc taaattttgc aataattcac ataaagaata attataaagc
    18001 tcaggatatt tttcaagagc agcatttaat ttatcaacat aagcttgata atgcttatca
    18061 tgatgcagtt tcattgtttc tttatctata taaggttcaa gtgcatcata tgcataaggt
    18121 aatggtttaa ccttaaattt gttattttca ggtgtaaaag caaatgaagt tatgcactgt
    18181 gagattataa ataaagacat aataactggt attaatattt ttttcttcat aaaaatcacc
    18241 tcctaatata gtatttctag aaataaaaaa aatatgaaat attaacagat aatttataag
    18301 aatttaatag caatttcatt ttaaattgaa taagttttaa agccaaagac aaaactatga
    18361 aatatagaat aagactaaag gacagaaagg aattttgatt atatggacgg caatatttca
    18421 atttattata aaggtgatat atctaaaaaa gaagatatta aatttaaaat aaaagaatct
    18481 gtaaataata caagactttc tagtagaata gagtataagg atgaggaaaa aacttttgat
    18541 tttgaaagat ttaatgtaat tgatgctatt tatggaaata aaccatttga aaaagatgga
    18601 aaagttattg tttttaatgg aaatatatat aactatcatg aaataaaaga agaattaatt
    18661 aaaaaaggac acaattttac tacaaatgat gatattgaag tcttattggc atcttatata
    18721 gaatttggta agaattgtgt acataagatt aaaggaatgt tcaactttat aatctatgat
    18781 agagaaaatg aaagtatatt tggagcaaga gatttatttg ggataaaacc actttattat
    18841 ataaataagg aaaatgcaat aattttttcc tcagagtata agtttttact tgaatatatg
    18901 aaaaatttaa atataaatga aagaagctta caaagttatt tttcatttca atatgtactt
    18961 cctgaagata ctatgataca aggaataagg ctgataccag caggtcacta ttttagggta
    19021 gaaaatggta tactatcttt aaagagatat aataaacttg agtttagaag tagtacaaaa
    19081 tttttttata ctaaaaatca cttaggcaat agagatgtga atgaggatga tataagaaat
    19141 atagtagtag attcaatagt aactcatatg gaagaggaga aagaaattgg aacatttctg
    19201 tctggtggaa tagattcatc tattataact actgtggctt cacaaattaa tccaaatata
    19261 aaatcttttt ctgcaggctt tagtgtcaaa ggatatagtg aattagaagt agcaaagaaa
    19321 acagctgata agttaggtat agaaaatatc cagattaata taacacaaga tgaatatata
    19381 aaatcattac cgaatgtaat ttattctttg gatgacccta tagcagaccc ttcagaagta
    19441 ggtctttatt tcttaattaa agaggcaggt aaacatgtta aagtagcatt gtctggagaa
    19501 ggagcagatg aattatttgg aggatataat atatataaag agtatagtac tatgaaatca
    19561 gttgtcaatt cacctactta tattaaaagt atattaggaa gagtttcaga acttatgcca
    19621 aacataaaag gaagaaatta tctttataga gctacaacac cattagaaaa aagatatata
    19681 ggaaatgcaa aagtttttga aaacagcgaa gtaaaaagat tcttttttaa gtataaggaa
    19741 aagaatatat atgaatacct gttgtctaat ttgtatagag atgcacaaaa aaataattac
    19801 gattatataa gtaaaatgca acatatagat gtgaatacat ggcttcaagg cgatatactt
    19861 caaaaaatta gcaaactttc tacagctgag caggttgaac ttagagtacc atttttatat
    19921 aaagatgtat ttgatgtagc aaaaaattta agaatggagc aaaaaataaa taagaataat
    19981 acaaaagttt tacttagaga ggcgtttaga gaaatagtcc cagaacatgt tgttcaaagg
    20041 aaaaaattag gatttccaac tccaataaga gtttggttaa aagattctct aggggacatt
    20101 gtaaaggaaa ctatttataa ttcaaatgta gatgaattta tagataaaaa atatgcaata
    20161 aaacttttag atacgcatct taaaggtcat agagataatt cgagaaaaat atggactata
    20221 tttacatttt gtctatggca tcaattattt attgagcata aaaatgtcga gtattaatac
    20281 ttttttcaat attaatcaga gggcaaaagc atgcaatgtt ttcaccctcc tgattattat
    20341 ggaataggat aagtagaatt ttgattgatt agtttactat ctcaataaaa ttattggcaa
    20401 atattttgac aagactactt tcaacagcta aatttaagtt ttgtccatca tcatatccag
    20461 cggaaatcaa tcctagtaag tttccaaaca tatctaaaag agctcctcct gaactaccac
    20521 tagagattgg agctgtaaat tgtatcattt gaaccgtttc aaaatcacga aaagctgaaa
    20581 taattccatc tgaaactgta ttaaaaagac ctaaagggct tccaattgca actatttttt
    20641 gacctctcac aggtttttta ctgactttaa caggtattgg cttgcatttt ctatcaacct
    20701 ttatcacagc taaatcataa tctgaatggt attttataat ttggtaagat gtataaactt
    20761 tattatcatt ttcaaatcta actaaaaatg aatacccatc attgactaca tgaagattcg
    20821 taagtatata accttcatta tttattacta ctccagaacc gctcttaaac ggtttatttt
    20881 cgttattgca cacaacaatc ataacaacag aagctgataa ctttgcaagg tcttcataag
    20941 aaagttctac ttcctcatct tgtgcaattt ttttagatgt tagatttgtg ttattaagtg
    21001 tatttttaga gttaggttta ttcgtagtag gagaagtctt attggtattt tcactgtaat
    21061 ttatatttgt aatatgcctt tcaaaaggta tttctaatat atttggagtc ttcctagaaa
    21121 gttcaaatag agtagaaaat tttgaacata caggatataa atcaagaata tctttatttg
    21181 gatttatata taaaatatca cacccaagtt gtgataaaaa atataagaaa aaaagttctt
    21241 gccttttaat atttccaaag tatactatct ttggtgaaat agtagtcttc accttgttat
    21301 aaaataattt aggtagaaaa tagtccatcc aacacaaaag tttaactgca aagtttttga
    21361 tgatagagga tgtacaattt ggcatacttg tggaaaataa atctaaagta tttttaaatg
    21421 caatcttttt agtccaattt aaagtgtcat tttctatttt acatggaaag tcgatattat
    21481 ataaatccca actgttaaac ttagaactat cataaatatt aaaaatttta agcatcttat
    21541 ctgtttgttc tttgtcaaaa tttattttta gattgtttaa tctaatatag ggaattgtag
    21601 gattattaaa aaaataagta tctatatttt taattttttc tatgtattta tcaattgaat
    21661 catcaacacc tatatatctt gtaaagtaga cttctttaat atatgaagaa tgcttatttt
    21721 tataggtagt atcagatata aattcaatta gaggttcagt tgactttttt attgaaatct
    21781 tt
//
LOCUS       DAEMHW010000051        21175 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEMHW010000051 DAEMHW010000000
VERSION     DAEMHW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.051"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            209..1633
                     /locus_tag="KPK67_001649"
     CDS             209..1633
                     /locus_tag="KPK67_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBG2458635.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDALFKRLESK"
     gene            1668..3797
                     /locus_tag="KPK67_001650"
     CDS             1668..3797
                     /locus_tag="KPK67_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG2458636.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDK"
     gene            3827..4249
                     /locus_tag="KPK67_001651"
     CDS             3827..4249
                     /locus_tag="KPK67_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2458637.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4268..5419
                     /locus_tag="KPK67_001652"
     CDS             4268..5419
                     /locus_tag="KPK67_001652"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG2458638.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEDILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5525..5626
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5809..6381
                     /locus_tag="KPK67_001653"
     CDS             5809..6381
                     /locus_tag="KPK67_001653"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBG2458639.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6445..7095
                     /gene="fsa"
                     /locus_tag="KPK67_001654"
     CDS             6445..7095
                     /gene="fsa"
                     /locus_tag="KPK67_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG2458640.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLYLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7247..10171
                     /locus_tag="KPK67_001655"
     CDS             7247..10171
                     /locus_tag="KPK67_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2458641.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKFERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIESEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            10760..11218
                     /locus_tag="KPK67_001656"
     CDS             10760..11218
                     /locus_tag="KPK67_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2458642.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            11279..11554
                     /locus_tag="KPK67_001657"
     CDS             11279..11554
                     /locus_tag="KPK67_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2458643.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            11613..12887
                     /locus_tag="KPK67_001658"
     CDS             11613..12887
                     /locus_tag="KPK67_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG2458644.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPK
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            13017..14069
                     /locus_tag="KPK67_001659"
     CDS             13017..14069
                     /locus_tag="KPK67_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG2458645.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            14069..15100
                     /locus_tag="KPK67_001660"
     CDS             14069..15100
                     /locus_tag="KPK67_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBG2458646.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKIGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVSKGFDCSGFMPAVNEALRMLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            15192..16004
                     /locus_tag="KPK67_001661"
     CDS             15192..16004
                     /locus_tag="KPK67_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG2458647.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            16007..16927
                     /locus_tag="KPK67_001662"
     CDS             16007..16927
                     /locus_tag="KPK67_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG2458648.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            17102..17548
                     /gene="rpiB"
                     /locus_tag="KPK67_001663"
     CDS             17102..17548
                     /gene="rpiB"
                     /locus_tag="KPK67_001663"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG2458649.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            17578..18243
                     /locus_tag="KPK67_001664"
     CDS             17578..18243
                     /locus_tag="KPK67_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG2458650.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            18353..19855
                     /locus_tag="KPK67_001665"
     CDS             18353..19855
                     /locus_tag="KPK67_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBG2458651.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRTYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(20167..>21175)
                     /locus_tag="KPK67_001666"
     CDS             complement(20167..>21175)
                     /locus_tag="KPK67_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2458652.1"
                     /translation="LAIVFLISIVVQAIIQYLVLIFFMYIYYAEAIAIVILFTLVNTV
                     FHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEVIGDDEISTLAKSINNITGGLE
                     TAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDMLKNNDFDRETINDYINILDKK
                     SQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQSIVEFEDKIQNSSLDFIIETP
                     EYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDMFIKEENIILTFKNISNDKLN
                     LKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENAHMEIIIDADLFKVMLTFRRV
                     N"
BASE COUNT         8582 a         2225 c         3917 g         6451 t
ORIGIN      
        1 tataaaaata gtattattta caaaattata tactccaaaa tactacataa ataaaattaa
       61 acaaaaacta aaatgacaaa tgtcattatc taaagtgaca taaaaacggt ttcttaaagt
      121 tgttactata aaatataaac tctataataa aagtataaca aaagggaatc atatcgatat
      181 agaatataat tcaagaggag gtaaaattat gagtagttct aatcaatcga caataaagac
      241 atctatacag cgagtaggga aatttttaag tggtatggta atgccaaata taggagcttt
      301 tatagcatgg ggtcttataa cagcattatt tataccaaca ggatggatgc ctaatgaaaa
      361 attgagtact ataggtgacc caatgataaa atacctttta ccactattga tagcttatac
      421 tggtggtaaa gcaatagcag gacaaagagg tggcgtaata ggtgcagcag cagctatggg
      481 agtaattgtt ggtgcagata taccaatgtt tatcggagca atgataatgg gaccatttgc
      541 aggatgggta attaagaagt ttgataaatt tgtagatgga aagataccaa caggatttga
      601 aatgttagta aataactttt caataggtat aataggtatg cttcttgcaa tactaggatt
      661 ttatgcaatt ggaccagcaa tagtagcagg gacagctctt atagaatctg gagttcaatt
      721 tatagttagc aaaagtttat tgcctctagt ttcagtattt atagaacctg gaaaagtatt
      781 attcttaaac aatgcaataa atcatggtat attagggcct ataggtatag ctgaggcaaa
      841 agaagcaggt aaatctataa tgttcttact agaaagtaac cctggaccag gtttaggagt
      901 tctattagca tattggatgt ttagtagagg aagtgtaaaa caatcagcac caggagctgt
      961 aataatacat ttctttggtg gtatccatga aatttacttc ccatatattt taatgaatcc
     1021 agttttaata ttggctacaa tggcaggtgg agcagcagga atattgactt tttcaatatt
     1081 aggagctggg cttgtagcag caccatcacc aggaagtata tttgcactta tggcactagc
     1141 tccaaaggga ggattacttc cagtattagc aggagttgca gtggcaacag ttgtatcatt
     1201 cttagtggca gcaccatttg ttaagagagc ttcagcaaat caaagtgaag aagacagtac
     1261 ttctttagaa gaagctaaag caaaaatgag tgatatgaaa tctgcaagta agaactctga
     1321 gaaaaatatt gaggaaaaac agcttgaagt aaatgagatt aagaagattg tatttgcatg
     1381 tgatgcagga atgggttcga gtgcaatggg agcttccaga tttaaaaata gaataaaaaa
     1441 cttagactta aatatagaaa ttacaaattc atctgttgat aatcttccag atgatacaca
     1501 aatagtagta actcacaata cattagttga aagagttgct aagaataatt caagtgttga
     1561 gatagtttca ataaacaatt ttttaaatga cccaaattta gatgcattat ttaaaagatt
     1621 agaatctaaa taaaaataaa aagtaccact aattaggagt ggataatttg aaaaaaaaga
     1681 agattacatc taggcaaaaa aagataatac taatgatagt agaaaattca aagaaaaaca
     1741 ttccaataac tatttctgag attgcaggga ctttagagtt aagttcaaga actgtactta
     1801 gagatatgtc tggaattgaa aaatggtttg atgaaaatga ttttaacttt gtaaaaaaac
     1861 caggagtggg attgatttta gaggaaaata tagaaaatca aaactttata attgagttgt
     1921 tagaagaaga aaaaatagaa aaagaataca gcaaggaaga aagaaattta attatattat
     1981 caaagctact agtttctaac gagccagtaa aatcctatta ttttgcaaaa atattaaagg
     2041 tatctgaagg ggtactgaat aatgattttg ccttagcaag taaatggttg gaaagatttg
     2101 atattgagct tgttagaaaa ccagggcttg gtgtctactt aaaaggtcaa gaaaaaaact
     2161 ttagagaggc atatgtaaat cttatatacg attcattcaa tgaaaaagaa atactagata
     2221 tggtcagaaa tataagtgaa aatatacaaa cagataaagc aatagaaatt ttaagtgaaa
     2281 atagactttt aaatttaatg gacaggtgca taataagaaa agttgaagct acattaacaa
     2341 agaagctttc agatttagat gtaaatttag ctgatagtgc atatataggg cttgtagttc
     2401 atatttcatt agccttacag aggataaaaa atggtgaaaa tataactatg gataaagagt
     2461 ttctaaaaga attatctata acagaagagt ttaaattagc taaagaaatc gtaaaaggta
     2521 tggaaacaga ttttaacatg gatataccag tagatgaggt tggatatata accatgcata
     2581 taagaggtgc aaagcaaagg tcatcttcaa accataaggc tttaaactta gatgatatag
     2641 aaataatgga aattacaaat aagatgatag atttggcgga agatgaattt aaaataagtt
     2701 taaaaaatga cgagaggtta tttaaagacc tagcaaatca cttagggcct tctataaata
     2761 gattgaatat gggattagaa ataagaaatc ctcttttaga tgaaataaaa agcaagtatt
     2821 catatgccta tgatggagtt gaaaagattt ctagaattat aaaagataaa ctaaatataa
     2881 attcaatacc tgaatcagaa ataggatata ttgcaatgca ctttgcatca gccatagaaa
     2941 aaaatcttat gatgaatact aatataaata ttgttgtggc atgtcccact ggaataggta
     3001 cttcaagatt cttatcaact aagattgaaa ataaatttcc taatttaaat atacttgaaa
     3061 ccatatcagc tataaacata gatgaagagt atttaaagga aaaagatgtt gatttaatag
     3121 tgtctacagt tgagttaaat acaagtttaa attatatatg tgtaggtcca tttatgagtt
     3181 tagatgatga acagataatc aaggaaaaaa taaaatctat agctcaaaat aaattaatca
     3241 atttaaatgc gaaaaatgat actaaaagta agaataaagt atatgaacag ataacagaaa
     3301 gtatgaatat aggaaaagat attcttcaat ttttagagga aattagattt gaaaaatttg
     3361 aaagtaaaga tttaagtgag cttattgaag actcatcaaa aatatttgca aaatctagtg
     3421 aagatataat ctctataaaa gaatcgttaa aagaaagatt aaaaatctca attccatata
     3481 tagaagaatc aaaaattcta ttgttacatt gtatgagtga aagaatagat attatgaagt
     3541 tatcaataat aaaactagag aatagaatcg tgctagattc aaatgaagaa atcgacaatg
     3601 tagtttttat gttacttcct aaaaattcac caagttatca aagacaaata atgagtgaaa
     3661 taagtggttc attaatagac aatataattt ttactaataa gataaataaa ttctctatag
     3721 aagagatgac attagaggta aaagatatag tttttaattt ttatacaaac agattaaaag
     3781 catttataga taaataaaat taatattaaa aaggaaggtt gatactatga gtaaagtatt
     3841 gaatgagaat aatatatttt tagggttaga tagtgtttca aaggaagaag ctataacttt
     3901 agcaggaaga aaattagttg aaaatggata tgtaaaagaa gaatatatac cagcaatgtt
     3961 agaaagagaa aaagtaatga ctacatatat gggaatggga gttgctattc cacatggtgt
     4021 aaatgaagca aaaaaagaaa tattatcttc tggaatagtt atattacaat tccctaatgg
     4081 tatagatttt gatggagaaa aagcatattt attaatagga atagctggtg ttggagatga
     4141 acatttagaa atactatcta atatagcagt agttttagat gatgacttaa cagaaagact
     4201 aaaaaattca aatgacaaac aagcttttat ggaagctttt gccaactaat cttaggaggg
     4261 atttaaaatg aaaaaggcaa ttcagtttgg agcaggaaat attggaagag gttttattgg
     4321 aggcttgtta gttaagtcgg gttatcatgt agtatttgct gatgtaaatg aggatatatt
     4381 aaactcaata aacaaagata aaaagtatac tatacacata agagatgtag aatgtataga
     4441 tgaagtaatt gataatataa gtgcggtttc atctataaaa gaagaaataa tagatgagat
     4501 tgtacaggct gaaataataa ctacagcagt tggaccttta gttttgacta aaatagcatc
     4561 tacaattgca aaaggtataa aggctagaaa agaaaaggga ttaacaagta atctaaatat
     4621 aatagcatgt gaaaatgcaa tatacgcaag ttcttcttta aaagaagagg tacttaagta
     4681 tttaaataag gaagaagtag aatatttaga gatgtatgtt ggttttccaa actgttcagt
     4741 tgatagaata gttcctcctg gtaaaaacga aaatcctctt gatgtaacag ttgaaaattt
     4801 ttatgagtgg aatgttgaaa aacaaggatt taaaggggaa ataccaacta tagttggaat
     4861 gaatttagct gataatctta tggcttatat agaacgtaaa ttgtttacac tgaatacagg
     4921 tcatgctata acagcttata taggatattt aaaaggatat aagactatag aagagagtat
     4981 aaaagataaa tttatctgtg atatagtcaa aagtgcaatg gtagaaagtg gagaaggatt
     5041 gattaaaaaa tacaattttg actcagaagt gcactataaa tatattgata aaattttaaa
     5101 tagatttaaa aatccatatc ttaatgatga tgtattaaga gtcggaaggg aaccattaag
     5161 aaaactaagt gataaagata gattgattaa acctttaatg acagcaaaat catatggatt
     5221 atcagttgat aatttgattc ttggaatagg tgcagcgctt cattataata atagtgaaga
     5281 tactcaaagt gtagaattac aagagttgat aaagtctatt ggggtaaaaa aagccgttgc
     5341 taaaatagct aatataagca atgacgaaga acttttaaat aatatcgaaa aaagttatat
     5401 atttatgaaa aatttataaa tttacttgaa aaaaagtagt tctgataaag taactataat
     5461 atattattct acacagtttg aattatttgt tgaataatag agaattataa tatataatta
     5521 aaaggaataa attaatatat ttacctgtat atgtttgata atatggttca aatgtttcta
     5581 ctaagctacc attaatagct tgactacagg agtgaagaag atatagttta tacttatttt
     5641 aaattatata ttaagttaat tactttgaat tttaagctcc tgtctatgat atagacagga
     5701 gcttttttag tattttaata taaaatactt acaagaatag atagcaaaat tagaacaaat
     5761 ataatttttt tattaatata attttagaaa gggctaggtg gaagttcaat gaaggcttta
     5821 caagaaaaga tattgagaga aggcagtgtt tcaggaaatg atatactaaa agttgatagt
     5881 tttttaaacc atcaaattga tgtagctttt ttaaatgaaa taggaagaga atttaaagag
     5941 agatttaaag gagaaaaggt ggataagata tttacaatag aggcttcagg aatagctata
     6001 gcatcaattg tttcacagta ttttgataat gcacctgtag tatttgcaaa aaaatcagag
     6061 tctaaaaatt tggataaaga tgtatatgaa actaatgtat attcatttac taaggcaaga
     6121 gagtattcag ttaaagtttc taaaaagtac ataaacaaag gcgaaaatat attgatagtg
     6181 gatgattttt tggcaaatgg aagagctgct cttggactta aagatttaat tgagcaagca
     6241 gaagctaact tagttggagt aggaattgtc attgaaaaag gatttcaagc tggaggagca
     6301 cttttaaagg ctaatgatgt aagattagag tctttggcag tagttgaatc tatagataat
     6361 ggaactgtaa aatttaggta atttatgata ttatatactt gtgtttagca catattttaa
     6421 ctatgtaatt atggaggatt ttttatgaga ttttttatag acacagccaa tattgaagaa
     6481 ataaaagaag caaatgattt aggtgtaatc tgtggtgtaa ctactaatcc gtctctaata
     6541 gctaaagaag gtagagattt tatagaggta gttaaagaaa taagtgaaat agtagatgga
     6601 ccaataagtg cagaagtaat aagtttagaa cataaaggta tgatagagga agcagaaaaa
     6661 ttatctaaga tacataaaaa tatagttata aagataccta tgacagcaga aggtctaaaa
     6721 gcggtgaaag tactatcaag taaaggtata aagactaatg taacgcttat attctctgct
     6781 ggacaagctc ttttagctgc tagagctgga gctacatatg taagtccttt tgttggtaga
     6841 ttggatgata tatcacaaaa tggtctttat ttaattgaag aaatcgttga tatatttagt
     6901 gtaaatagta ttgaggctca aataatagtt gctagtgtta gaaatccaat acatgtttta
     6961 caagctgcta gaatgggagc agatatagca acagttcctc ttaaagttat aaaccaaatg
     7021 attaagcatc ctttgacaga caaaggaata gatagcttta tgaaggattg ggaaggtgct
     7081 tctttaaaat tataattata aactacgctt tataagtata ttaaagaggt tattatggaa
     7141 attctaagta ataaggctag gctattttgc ctagtcttat tttggtgtat aatatattga
     7201 aacatattat tgaaatagat taatttttta agtgaggagg cgaaaaatgg ataaaatatt
     7261 aagagagata caaagagaag ataaaaaaaa tccatatact gaccaagaac ttgctgaaat
     7321 gttaaatgta gctaggtctg aagtgatatc agttaggaaa aaaaataata ttttagattc
     7381 gagagaaaga agaaaaaaaa tattaataaa agatatatca aaaattgtaa gtgaaaatcc
     7441 tcaaatgtct gaaagaaaaa taactgaaat attaatagaa aatggatata atatatcgag
     7501 aagtgcagtt tcaaaattat taaaagagga aaatttaaat cagtcaaaga ttgggattaa
     7561 ggataaagac ccagcatcat caggaaagta tgtgaaggtg agtaaagttg tagaagaaga
     7621 tggatttgaa gaattgatag gtataaaggg aagtttgaaa gaaaaggtaa atctagctaa
     7681 gtcagcaata atgtatcctc ctaatggcct tcatactata atatatggag aaacaggagt
     7741 aggtaaaagt gaattagcta catgtatgta taaatatgct ataaaaaaca acattaaaga
     7801 agagaacagc ccatttatag tgtttaactg tgcagactat gcagaaaatc ctaatctatt
     7861 gatagctcaa ctctttgggg tagtaaaagg tgcatataca ggagcagatg caaatagaga
     7921 aggtcttgta gaacaagcta ataatggaat attgttttta gatgaaattc atagattgcc
     7981 tgctgctggg caagaaatct tattttcgtt aatagataga ggagaattta gaaggttagg
     8041 agaaagtagt tctaatagaa aggcaaatgt tctcataata agtgctacaa cagaaaatcc
     8101 agattctaat ttacttcaaa catttagaag acgtatacca atggtaataa atttacctaa
     8161 tatgagtgaa agaccaaaat ttgaaagata tgaaataata ataaagtttt ttgaaagaga
     8221 agcaaagcga gtaaacaaga attttataat aactaaagaa gttatgtgtg cacttatgaa
     8281 ttataagtgt actggaaata taggtcagtt aaagagtgat atacaagtaa cttgtgctag
     8341 agcttttagt aaatcgatat tttctagcga taaggtaatt atagatttag atagtttaag
     8401 ggattatata aaaagtggat attatgattg taattgtaaa aatgaaaagt attctgagtt
     8461 ttcagttgag gatatatata tagatattag tgaattggtg agtggaaaaa atagaatttt
     8521 atctgataat gagataagcg agatatataa ttatgcagaa aaagagctta aggttttgga
     8581 aagtaagtgc ttttcagaag aagagttaaa aaatgcattt atagaaaagt tagataagaa
     8641 gtttaatgct attaaaaaca ataaaaattt atctgataga agaagtcgaa tagactgggg
     8701 aggacaatta aaagagtcta cactggagat aatggataag gtaattgtat ttattaagga
     8761 gcagtttaag catgtaaatc aaggtctgta cttggctcta gcaattcata ttgagcatgc
     8821 tataggtagg ataaaagatg gaaaaactat aataaatccg agtttagata aaataaaagc
     8881 atcaatgcca atggaatatg agttgtcaag atatataatg ggtatagtag aagatttgac
     8941 acaagtaaca tttccagaag atgagcttgg atatttggct tattatataa ataaattttg
     9001 ttataatgag gaaagtataa aagataaagt taaagtagta atagttacgc atggtaaagt
     9061 tggaattgaa atgtctaaag ttgtaaatca tattttaggt atagaatgta cactaggtat
     9121 agaaatagca cttacagatt caccatcaga ggggatagag catgtattag aagagcttca
     9181 aaagatagaa gctagaaaag gtattctagt tctaattgat atgggttctt tggtaattct
     9241 tggtgatgag gttgaaaaaa ggcttggaat tagatgtaaa actgtaaata gagtagatac
     9301 attattggca atggaagcag gaaaacttgc aaccattgaa gggaaaagtt tagatggtat
     9361 aatagctgat ttaaagaaaa ataagaatta tgctatgata aatacaaata aatttagtta
     9421 cagaaaaaac gagtatggaa agaaaaatgt aatcattact ttatgtttga gtggtgtagg
     9481 tacagcttta aatttaaaag agcatataga aaaacaaatt aaggaatatg acactttaat
     9541 tgaagtaaag ccagttgctt ttttaaataa taatttagaa gaagagttaa atgatattga
     9601 atctgaatat aatattattg ctatctgtgg aactattgat atagattata aaaacgtgcc
     9661 ttttatttct tataatgagg tacttggtaa aaatggtatt aataaagtat tagagcattt
     9721 aaatagtaaa caaccagctt taaatattga aaaccagttg aataatttaa tacatgaaga
     9781 tttgatttta tacaattttg aaggaatttc aaaggagtat ataattgata ctcttgtttc
     9841 aaaacttgaa gaaggtggat atgttgactc aaagtatata ttaagtgtat ataaaagaga
     9901 agcaatggga agtgttgtaa tggcctctaa ggtagccgtg ccacatggac ttccagaaaa
     9961 cgttataaag cctgcaatag caatcgcgag gttgaataaa cctatagtat gggacaataa
    10021 atttatggtg gatttggtgg tactactggc acttaaagaa aataataaga aagaaatacg
    10081 aagcttattt tcaaagatta atgaccagca tactttagaa gttttattaa atactgaaga
    10141 taaaaatgaa ataaaagaac ttttaagcta attattagta ccgagaagaa agacaaaaaa
    10201 atttgcttta gtataaaatt taaaactact caattgctaa gtagttaatt actctatata
    10261 aggctagttc tatgaaattt atttatatga ttttatagaa ttagcttttt tcttggtaaa
    10321 taaaatatat gaatttattt atgtaaaaag taaattttat tggacaattc tatgagaatt
    10381 tttcctatat ttcttataaa atatagaata tataaaaaat atataaaaat aaattaaatt
    10441 cataataaat aattttataa aatagttgtt aaacataaaa aaccatttgt aaaaaacaaa
    10501 aaaatcattt ctctaacaaa aaaaaccaca aaaaatataa aaaagtatgg ctagttaagt
    10561 gattaacaag taaaaattaa aataaaaatc aaaaaaaatt tatcaatgaa agtcagctaa
    10621 aatcctggaa gagagcattg tttttgggaa aacattttct tttgtttcac aaaaaaagcc
    10681 actttttggc acacaatatg ctttacataa agtcaagaaa aactaaggta taagacaaag
    10741 gcaattagga ggtgtaaata tggagtttat aaattttgta agtgataaag ttatagacct
    10801 taaaatggat tgtaaaacga aagaagattt ctttaaagaa atacataata aagtttttga
    10861 tttaggcttt gtaaaagaag aatttggaga aaaaatatta gcaagagaaa atgtatttcc
    10921 aacaggttta aatttaggtg attatggagt agcaatacca catacagatg cagaatatat
    10981 taaagagcaa tttatatcag tctgtacttt taatgaacca gtagtattta gttctatgga
    11041 agaccaagat gaaaaagtac ctgtaaattt ggctttcgta ttaggactta atcaaccaca
    11101 cagtcaatta tctgtattaa cagaattaat gggaattatg caaaataaag agttagttga
    11161 taagttgatg agttctactg ataaagaaga agttttaaag acactaaagt ctttataaaa
    11221 ataaaaaagt actaaaaata aaaaatataa aaataaaaat tattaggagg aaataaaaat
    11281 gaaaaaaata ttagtagcat gtggagcagg aatagcaaca tcaacagtag tttgtgacaa
    11341 ggtagagaga ttagttaagg aaaataatat acaagcagaa gttatacaat gcaaaatagc
    11401 tgaatgtgca acaaagcaag aaggagcaga tttaatagta tcaacaacta tactaccaac
    11461 aacttataat ataccagcaa taaaagcaac agcatatata actggagtaa atacagcagc
    11521 attagataaa aaaatattag atgctttaaa ataatcctat catagataca ttgaaataat
    11581 attttataaa acagaaaaag gaggatttat aaatggataa attattagca gtagtacaat
    11641 acattttaaa tatgggaccg acagtaatac taccaataac aattgtagta ctaggaatga
    11701 tatttagaat taagtttaca aaagctttaa agtcaggtct tacaattggt ataggatttg
    11761 tgggtataaa cttagttgta aacctattaa catcaacact agggccagcg gctcaaggaa
    11821 tggttgaaag attcggactt aatttaacag taatagatgt tggttggcca acaacagcat
    11881 cagcaacatg ggcttcacca gttgcaccaa tattaatacc agtttgttta ttagtaaact
    11941 tactattatt atacttcaat aaaacaaaga tattaaacgt tgatatatgg aattattggc
    12001 actttatagt agcaggtgca gttggatata tagtaacagg aagttatgtt tgggctatat
    12061 tctgtgcaat actaatggag ttattagtac tgtttattgc agaaaagaca gcaaaaaatg
    12121 ttcaggagtt ttatgattta gaaggtatag cattaccaac tggttcaaca gcttcatttg
    12181 ctccacttgg atatttagta ggtaaatttg ttgaaaaaat accaggaata aataaaatac
    12241 aagcagaccc agactctata caaaaaagat ttggtatatt tggagaacca atgatgatgg
    12301 ggattatact aggtataata cttggagcac ttgcgggata tgatgtagca ggaatattca
    12361 atgtaggtat atcaatgggt gcagtaatgt tacttatgcc tagaatggtt agaatattaa
    12421 tggaaggttt aattccaatt tcagaatcaa taagagattt cttacaagca aaatatggag
    12481 acagagaact ttatataggt ctggatgcag cagtagcaac tggacatcca gcagttatat
    12541 caacagctct tatacttgtt cctataactt tgttcttagc agttatacta ccaggaaaca
    12601 aagttttacc atttggagac ttagcgacaa taccatttta tatggcattt atagtgtgct
    12661 ttagaaaagg taatatagtt caatcagtta taacaggaac aatagtaata gctatatcac
    12721 tttatatggc tacaaacttt gcagatgttc atacacaatt attacaacaa gcaaatatga
    12781 tgccaaaagg aacaacaatg gttacaagta tagatacagg tggaaacttg ctaaaatggg
    12841 gaatattgaa attatctcaa ttagtttctt catttggaat attctaatat gtagttaata
    12901 gagattactc ttttttagta aagtatgcga aaatttaaaa tttagcagct actaaaaaag
    12961 agtaatattt tttataaatt tttaaaaata caaaatacaa gaggaggaga tttatcgtga
    13021 aagcagcagt attacatggc acaaatgata tgagatttga agatatagaa ataaaaccat
    13081 gtgaaagtga tgaagtaaaa ataaaagtta tggcagcagg aatatgtggg tcagatcctc
    13141 caagagtatt aaaacattgg aaatatccag ttccagctat accaggacat gagttttctg
    13201 gagtaattgc tgaagttggt aaggatgtaa aaaatgtaaa agttggagat agagtagttg
    13261 caattccttt tataccttgt aatgaatgtg aatattgtaa aagaggatta ttttcattgt
    13321 gtgatgacca tggaatgtta ggagctaaaa gttttggagc ttttgcagag tatgtaaata
    13381 taaaagctac aaatgtactt ccaataggag atatggactt tgaagatgct gcaatgatag
    13441 agccactagc agttgctatg catggagtac ttaatatagg tgttcaagtt ggagacactg
    13501 tagccgtaat gggtagtgga acaatggggc agttggtaat acaaggactt aaaatagcag
    13561 gggcaggaac aattatagca gttgatatat ctgataataa attaagagaa tcaaaggaat
    13621 taggggctga tataataatt aatgctaaag atattaaccc agtagaaaaa ataaaagaac
    13681 ttactggtgg aaaaggcgtt gacatagctc ttgaatgtgc aggttcaaaa ataactcaag
    13741 aacaatgttt acttataact aaaaagaaaa gtaagatagg atttttaggg atagcatatt
    13801 cagatataac tttatcagaa gaagcatttg aaaatatatt tagaaaagaa ctagaattaa
    13861 aaggtttttg gaactcttat tctgcaccat ttccaggaca agaatggact aagggaatca
    13921 atctagttaa tgaaggaaag ataaaattaa aagaaatggt atctcataga ttctcacttg
    13981 aagatactta caaagcattt gaaatgataa gagatagaaa agaagaattt aacaaaatat
    14041 taattttacc acaaggggtt gaaaaataat gaaagcagta gttaaaacta aaccaggcta
    14101 tgataatgta gaagttttag aagtagaaga accaaaagct acaggagaca aggttaaaat
    14161 aaaagtagaa tatagtggta tatgtggttc agatatacat tcatttaaag gtgaatatgc
    14221 aaatataaaa gccccagtaa cattaggtca tgaattttct ggaattgttg tagaagtagg
    14281 agaagatgtt aaaaatataa aaataggaga tagagttaca tcagagacta cttttgcaac
    14341 ttgtgagaaa tgtatatatt gtgaaactaa agattataac ttatgcccaa ctagaaaagg
    14401 tattggtact caagtaaatg gaagttttgc tgagtatgta ttatcaagag aagaaagcat
    14461 acatgtactt cctgaaaatg tatctttatt atcagcagcc cttacagagc cactagcttg
    14521 ttgtgttcat gcagctcttg aaaagactac tatagaaagt tctgatacag ttttaataat
    14581 aggaccagga ccaattggtt tattattagc tcaagttgtt aaatctcaag gtgctacagt
    14641 tataatgtct ggtgttacaa aagataagga aagattagaa atagctaaaa atcttggagt
    14701 agatagaact gtaaatgtaa tggaagaaag tttggcagat gtagtgaatg aaatgactga
    14761 tggaaatgga gtaagcaaag gatttgattg ttctggtttt atgccagcag ttaacgaagc
    14821 cttaagaatg cttagaaaaa aaggtacttt cgtacaagtt ggaatctttg ctaaaaaatt
    14881 aaatgaaatg gaccaagaag caataatcca aagagagtta caatatatag gaagtagatc
    14941 tcaaaagcca agttcttgga ttacagcatt agatttaatg gcaagtggga aagtaaatac
    15001 agaagcttta gttactagaa ctgttggttt agacgaattt aaagaaggta ttacagcttt
    15061 aatgaacggg gaagaaataa aagtagcagt aaaatcttaa aaaaacattt gtattatttt
    15121 aaaaaaaatt gtaaaatcta ctgtaggata aaaaaatgta aggtcttaaa aaataacagg
    15181 aggaagaaag tatgagcact cttagagatc attctaataa tattagaaaa aacataataa
    15241 aaatggttgc agaagctaaa agcggacatc caggtggttc attatcaata gctgatatgt
    15301 taacagtatt atattttgat aagatgaacg tatgtgcaga aaatccaaaa atggctgata
    15361 gagatagatt tgttctatca aaaggtcatg cagcaccagc tttatatgct acattagcag
    15421 aaaaaggatt tttcccagaa gaagaattaa taacactaag aaaatttggt tctaagttac
    15481 aaggtcaccc agacatgaaa aaagtagcag gaatcgatat gtctactggt tctcttggtc
    15541 aagggctatc tgcagcaaat ggtatggcac ttgcaggaaa attagataac aaagactata
    15601 cagtatatac tatattaggt gatggagaaa tccaagaagg tcaaatatgg gaagctgcaa
    15661 tgacagcatc acattacaaa ttagataatt taattgcatt tgttgactta aatggattac
    15721 aaatagatgg ttctaatgaa gaagtaatga atgtaaatcc agtagatgac aaattcaaat
    15781 cttttggatg gaatgtaata gtaataaatg gacattcttt tgaagaaata ggaaatgcag
    15841 ttgatgaagc taaaaaagtt acaggaaaac caactgtaat aatagctaaa acagtaaaag
    15901 gtaaaggtgt ttcatttatg gaaaataatg ctgcatggca tggaacagct ccaaatgcag
    15961 aacaagcaga gcaagcattg aaagatttag aaggaggtct ataggaatgg gaatagcaac
    16021 tagagaagct tacggacaag tgttaaaaga acttgcagaa aataaagata tagtagtatt
    16081 agatgcagat ttaggaaaag ctacaaaaag tatatcattt aaagaagttg ccccagatag
    16141 attttttgac atggggatag cagaaggtga catgattggt acagcagcag gtcttgctac
    16201 ttgtggaaaa ataccttttg cgagtacttt tgcaatattt gcagcaggaa gaggctatga
    16261 gcaaataaga aactctgtag catatccaaa tttaaatgta aaaatagcag ctactcatgc
    16321 aggtgtaaca gtaggtgaag atggtggtag ccatcaagct atagaggata tatctctaat
    16381 gagaggtata ccaaatatgg ttgtattaaa tccagcagat gctttagaag caagacaagc
    16441 tattcttgca tctattgatt ataatggacc tgtttatata agattaggta gagctgcaac
    16501 tcctgatgta aacagtgaaa attataaatt tgaaatagga aaaggaactg ttttaagaga
    16561 aggttctgac ataacagtaa tagctacagg aataatggta gcaaaagcct tagaagcagc
    16621 agaagaactt gctaaagaag gtataaatgt agaagttgtt aatatatcta caataaaacc
    16681 attagatgaa gcattaataa aagaaactgc taagaaaact ggaaaagtag ttacagcaga
    16741 ggagcacagt ataataggtg gtttaggttc agctgtatgt gaagctttag cagaaactaa
    16801 agatgtagta gttagaagaa ttggtgttaa agatgtattt ggtcaatcag gtacaccagc
    16861 tgatttatta aaacactatg gtttaactac agaagatata gtaaaaaata taaaagagtt
    16921 gttatagata taaatatatc taaataataa attgatttga cataagtcaa ttaaagctgt
    16981 cttaaaaata gaaagaaatt tctacaatgg gacagcttta tttttacaat actttatttt
    17041 tatgataatt ataaaatagt ctgaaagtaa tcaaaaaaat tataaaaaga ggtgctattt
    17101 aatgaaaata tctattggat gtgaccatgg tggttatgaa ttaaaggaat atataaagga
    17161 aaatttaata aataaaggta tagaagttga agatgtgggt acaaattcaa aagaaagtgt
    17221 agattttcca atatatgcaa aaaaggtaag tcaaaatgtt caaagtaaaa aatctgattt
    17281 aggcatactt tgttgtggaa caggaatagg tatgtctatt gcagcaaata agtttaaagg
    17341 aatcagagca gctgttgttt ctgactgttt ttcagctaag gcaactagag aacacaataa
    17401 tacaaatgta ctttgtttag gagaaagagt tattgggaaa ggattagcaa tgagaattgt
    17461 tgaagaatgg ttaaattcaa gttataatgg agaaagacat gcaagaagat tacaaatgat
    17521 tgatgatata gaaaaagaaa atttataaat atataattta taacaagggg gagcgttatg
    17581 tctattattt gtgcttcaat aatgtgtgca gaccaaatga agttaaaaga ggaattagaa
    17641 gctcttgaag aagcaggtgt aaaattactt cattgtgatg ttatggatgg aatatttgtt
    17701 aaaaacttgg ctatgggacc agagttatta aaatcaataa gtgaaaatac aacaatacct
    17761 ttagacatac acttagccac agaaactcca gataaatata ttgatatgat gtcttatata
    17821 aagccaaaat atatttcttt tcatgtagaa tcaagtacaa atgtaaaagc tgatatacaa
    17881 aagttaagaa attatggtat agggccagtg ttagcaataa gccctcaaac aagtgtggat
    17941 aaaatagaag aatatataag tttagttgag ggtatcttag taatgacagt aaatccaggg
    18001 tttgcaggtc aaaagtttaa tctttctgta ttggataagt tggataaact tacagagata
    18061 ctaaaagatt atgataatcc tccatttata gaggttgatg gaaatataaa taaggatact
    18121 attaaactta tgaatggtaa aaaagtagat atatatgttg ttggtacttc agctttattt
    18181 aatgataagc ctccaatttc atataaagat aaaatagaag aactaaaaga aagtataaag
    18241 taaccatgta ataaaattat aatatgtttc tgtttaatag taaaaattta ggtggaaaca
    18301 agaaatttta taatatttaa taaaaatttt ggttgagtaa ggagaatttt ttatgggaga
    18361 cctatataaa ctacaaaatg gtactgatat tagaggaata gcttatgaaa atgattctaa
    18421 agaagttaac ctaactgtag aagaagtaaa aaaaatagct aaagcgtttc atatttggtt
    18481 aaaagaaaag actaagaaag ataaagtaac tgttgccata ggaactgatt ctaggataac
    18541 aggaagccaa tttagaagta ctgttattga aactttaact aatgatgatt gtaatgtaat
    18601 tgattgtgag attgccacaa ctccagctat gtttatgaca actattatgg atggatacaa
    18661 ttctgatggg gcgattatga ttacagcaag tcacttacct tattactata atggtttaaa
    18721 gtttttcaca gaaagtggag gtttagaaaa aactgatata aaagagatgt tggatatagc
    18781 tgttaaaaat gatagtgtag actatgagga agtaaataaa aaaggtgaag ttatagtaaa
    18841 aaatttaata gaagattatt ctaatttatt gatagataaa attagaaaag gtgtaaattc
    18901 aagtaaaaat tatgaaaagc ctttttctgg gcttaagata ttggttgatg caggaaatgg
    18961 ggctggtgga ttctttgctg aaaaagtttt acacatactg ggagcagata cgacaggtag
    19021 tcagtttttg aacccagatg gtatgttccc aaatcatata cctaatcccg aaaacaaaga
    19081 agcaatggaa agcatatgta aagctgtgtt ggataataag agcgacctag gaataatctt
    19141 tgatacagat gtagacagag ctgctattgt aggtaaaaat ggcaaaccaa taaataaaaa
    19201 tgctcttata gctgttatat catcaatagt attagaagaa catccaaaaa cagctatagt
    19261 aacagattcg attacttctg aaggacttgc taaatttata aatgaattac aaggaagaca
    19321 tcatagattt aaaagaggat ataaaaatgt aataaatgaa gctataagat taaatagtga
    19381 aggtgaagag tgccatttag ctatagaaac gtctggacat gctgctttaa aagaaaatta
    19441 tttcttagat gatggggcat atttaatagc taaaatttta ataaaagtag caaagctatc
    19501 cttggaagga aagactatag aagaattgat agaaaacctt gaagaagctc aagaagagaa
    19561 agaagttaga ataggcatta ataaagaaga ttttagaaca tatgcagagg atatattaaa
    19621 agatttagat tctcatgtta agagtattga tggatggtct gtagcaccaa ataattttga
    19681 aggcataaga gttaattgtg atgaatcaaa tggagatgga tggtttttac ttagaatatc
    19741 actacatgaa ccattattag ctttaaatat tgaatcaaat aaaattggcg gagcagaaca
    19801 aatttattct aagttaaaac ttttcttaga aaaatatgat ttaaaaggtc tttagggtaa
    19861 atagaaagaa tatatttata ttgaagaata aatgtaaatt tgtacctaag attacaaata
    19921 ttacaaaatt gtaatcttag gtaacagaga tgtaactgat tatggaaaca ctatgtgata
    19981 ttatagatac agagagatga aatatgtgat gaattataga gaaatataat ttaaaatgta
    20041 ttaatatcac accaaaaagg tataatcaaa aaatatgtaa atataaaccc caacatttag
    20101 aaaaagacct ctaaggaggt ttttttcttg tttaaataac ttaaaatata tttttatatt
    20161 aatatatcaa ttaactcttc taaaagtaag cattacctta aataaatcag catctataat
    20221 tatttccatg tgtgcatttt ctaattcaat tatattttga gcaattgata aaccaagacc
    20281 agagccatct gtttttctag atatatcacc ccttctaaat ctttctatta actcttctgg
    20341 ttttagattt agtttatcat ttgaaatatt tttaaacgtt aatattatat tttcttcctt
    20401 tataaacata tctatataga ccctagtgtt aggcatagag tattttagta tattgcaaat
    20461 tagattctca aaaactctcc aagttttttt accatctatc atggtaaaaa cagcatattc
    20521 aggtgtttct attataaaat ctaaactgct attttgaatt ttgtcttcaa actctacaat
    20581 actttgaatt aaaagttctc ttatatcggt ttttgttata ttaaattcta catctccact
    20641 acttagctta gatgcttcaa atatgtcatc tattaataat tttaatcttt gtgatttttt
    20701 atctaaaata tttatatagt catttatagt ctctctgtca aagtcattat tttttaacat
    20761 atcaacatag cttaatatag atgtaagtgg agtttttaag tcatgagata cgtttgtgat
    20821 aagctctgcc ttcatccttt cattttttat attagcatct acagcagttt caagaccacc
    20881 tgttatatta tttatacttt tggctaaagt agatatttca tcatctccta taacttctaa
    20941 tttatacttt aaattgccat tttctatatt ttttatattt tttgatatgt tatttatgta
    21001 attaaactta acagataaaa agttgtatat ataaaaatga aaaactgtgt ttacaagtgt
    21061 aaataaaatt actatagcta ttgcctcagc ataatatata tacataaaga atatcaaaac
    21121 aagatattgg ataatagctt gtacaactat agatattaaa aatactatag ctaat
//
LOCUS       DAEMHW010000052        21163 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEMHW010000052 DAEMHW010000000
VERSION     DAEMHW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.052"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..965
                     /locus_tag="KPK67_001667"
     CDS             <1..965
                     /locus_tag="KPK67_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG2458653.1"
                     /translation="FDNKTEEECLELVRKNGLNLIYIKNQTEEICLEAVRQNCNAIKC
                     VQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTALKYIENQTEELCLRAVE
                     KNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEAVKHCSYNLKWVENQTE
                     EICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQTVGICLKAVRQYGMAL
                     KFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPFAKRYVRNVIEKENRAL
                     IKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLGVF"
     gene            1101..1589
                     /locus_tag="KPK67_001668"
     CDS             1101..1589
                     /locus_tag="KPK67_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2458654.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            1765..3693
                     /locus_tag="KPK67_001669"
     CDS             1765..3693
                     /locus_tag="KPK67_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2458655.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            3870..4280
                     /locus_tag="KPK67_001670"
     CDS             3870..4280
                     /locus_tag="KPK67_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBG2458656.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            4302..4604
                     /locus_tag="KPK67_001671"
     CDS             4302..4604
                     /locus_tag="KPK67_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBG2458657.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(4821..5513)
                     /locus_tag="KPK67_001672"
     CDS             complement(4821..5513)
                     /locus_tag="KPK67_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG2458658.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(5539..6726)
                     /locus_tag="KPK67_001673"
     CDS             complement(5539..6726)
                     /locus_tag="KPK67_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2458659.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            7062..8939
                     /locus_tag="KPK67_001674"
     CDS             7062..8939
                     /locus_tag="KPK67_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2458660.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNVGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVFAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGWLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            9251..10657
                     /locus_tag="KPK67_001675"
     CDS             9251..10657
                     /locus_tag="KPK67_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG2458661.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     VAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            10685..11533
                     /locus_tag="KPK67_001676"
     CDS             10685..11533
                     /locus_tag="KPK67_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2458662.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            12194..12886
                     /locus_tag="KPK67_001677"
     CDS             12194..12886
                     /locus_tag="KPK67_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2458663.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            12883..13818
                     /locus_tag="KPK67_001678"
     CDS             12883..13818
                     /locus_tag="KPK67_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2458664.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIIYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREECLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            13966..14877
                     /locus_tag="KPK67_001679"
     CDS             13966..14877
                     /locus_tag="KPK67_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2458665.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRNLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGG"
     gene            14874..15644
                     /locus_tag="KPK67_001680"
     CDS             14874..15644
                     /locus_tag="KPK67_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2458666.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILFFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            15654..16436
                     /locus_tag="KPK67_001681"
     CDS             15654..16436
                     /locus_tag="KPK67_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2458667.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            16813..17349
                     /locus_tag="KPK67_001682"
     CDS             16813..17349
                     /locus_tag="KPK67_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG2458668.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            17381..17770
                     /locus_tag="KPK67_001683"
     CDS             17381..17770
                     /locus_tag="KPK67_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG2458669.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            17807..18193
                     /locus_tag="KPK67_001684"
     CDS             17807..18193
                     /locus_tag="KPK67_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBG2458670.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            18320..19762
                     /locus_tag="KPK67_001685"
     CDS             18320..19762
                     /locus_tag="KPK67_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG2458671.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKIRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            20059..20874
                     /locus_tag="KPK67_001686"
     CDS             20059..20874
                     /locus_tag="KPK67_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2458672.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         7807 a         2482 c         3877 g         6997 t
ORIGIN      
        1 attttgataa taaaacagaa gaagaatgtt tagagctagt aagaaaaaat ggattgaatt
       61 taatatacat aaaaaatcaa acagaagaga tatgtctaga ggctgttaga caaaactgta
      121 atgcaataaa atgtgttcag aatcagacag aaaaaatatg tatagaagca gttaaacaag
      181 attggagaat gttagaattt gtggaagagc caacagaaga aatttgcatg ttggctatta
      241 accaagatgg aacagcctta aaatatatag aaaatcagac agaggagtta tgcttaagag
      301 ccgttgaaaa aaatggtgcg gctttggagt atgtaaaaga acaaacagaa gaaatatgta
      361 tagaagcagt tagaaacagc gaatttggat tggcaagagt gaaaaatcaa acagaaaaaa
      421 tatgtatgga agcagttaag cattgtagct ataatctaaa atgggtagaa aatcaaacag
      481 aagaaatatg tatggaagca gtgagacaaa atgggcttga tttgaaattt gtaaaaaatc
      541 aaacagaaac aatctgttta agagcagtga gacaaaatgg aatggcatta gaatttgtaa
      601 aggaacagac tgttggtata tgcttaaagg cagtaagaca atatggaatg gcattaaaat
      661 ttgtaaaaga acaaacagaa gaaatttgta cagaagctat aaaacaagat aaaagagcac
      721 ttagttttgt aaaaggagat aaggaaaaat acaaggctct atatgataat aatgaaccat
      781 ttgcaaagag atatgttaga aatgtaatag aaaaagaaaa cagagcattg attaaaaaag
      841 gagaagaaaa tgcaaaaatc aaaattgcag gaaaacttta tgatagaggc atgtcttttg
      901 aagatatatc agatatagtt gagattgaaa taagcaagtt aaaagtgagt ttaggtgttt
      961 tttaaagtaa taaatgtagg tgttttttat gggaaatagt taataaaaaa ataatcgact
     1021 agctctttgt aggacagcaa aattagtgat ataatttgat gtataataaa ttatttttat
     1081 tagataaggg ggaattaaag ttgaagatta cagtattata ccaaacaaga agtggaaaca
     1141 cagaaagggt cgctaagctg atagaagatg gagctaaaaa ggtggatgga gtagatgtaa
     1201 agcttatgag acttgataat attgatttag attatttaaa tgaaagtaaa gcagtaatat
     1261 ttggaacacc tacttattta gcaaatacaa cttgggaagt aaaaaaatgg tttgatgaag
     1321 attcaaaaaa agttaattta gctggaaaat taggagctgt ttttgctaca tgtgattata
     1381 tatgtggagg tccagatgtt gcgatactta ctatagtagg acatttgatg gttaaaggga
     1441 tgcttgtgta ttctggaggt ggttctttag gaaagccatt tatacattta ggacatgtac
     1501 attcaattga aggtccagag ttacaagatg agaaggctat aatatttggt gaaagaattg
     1561 ctactaaggc aaaagaacta tttgcttaat ttctatatgc ataataatga ttagagtaaa
     1621 tttagggttt taatggagat ttgaagtaaa agttataaaa taaaaaattt tccataaaat
     1681 tactcaagat gaattataat ataattataa ataaattata ttattaaaat atttaaaatc
     1741 aaacgtcaat agcgagggaa gtttatggta tggcatatag aatatgagat tttttctgca
     1801 attatagtta tttttttaat ggtatacttt tttagaagta aatttatacc tactttacaa
     1861 aacaaaatat attgtgcact tttaatattc tcatttttat ttataatttc aaatatttta
     1921 ggttcatttt gtctaaataa tatagacaaa attccaattt ttattacttt tttattgaat
     1981 cagatatact tattgttatt gccaatacca gcagcacttg tatcatttta tgttatggct
     2041 attatttatc aagatataag gtatatgaaa aaaagattat tatttttatc tataccactt
     2101 ataatttgta taatcttatc tattacaaat ccttttacgc atattctatt tggattgtca
     2161 aaagaaacag gttatatacg aggttgtgga tatgttgcaa cttttgcatc ttttttcttt
     2221 tatgcaatat attcagcatt tttatctttt aaatatagaa aagcaatgca tgaatcaaaa
     2281 atatgggcta ttagaggttt tcttatagtt tcaattgtgg caattatctt acaggcaatt
     2341 tttttacagt atcttcttac aggaactgca tgtgcatgta gtttactttt gacatatctt
     2401 tctatgcaga atcgaggtct tattattgat gaccttactg gtgtactcaa tagacaatct
     2461 tttattcaag agctagattt gaatatcaat actgaacaat caggatttat tattacaatt
     2521 gcgcttgatg attttaagtt tatgaatgaa acttttggaa caaaaaatgg agatattgca
     2581 ctaaaagaag ttggaaaata tttgattcag atacatgatg taaaccatgt atatagaata
     2641 gggggagata tattttctat tgttgtcaat agtcaattag gtatagaacc agatgatatt
     2701 atcagaaaga tagaagaaag atttaaaaag ccttggctag tagaagagat aagttttaat
     2761 ttagcaacta gtattgctgt tgtttattac ccagaaaatg cagaaacgac agaagatgta
     2821 attgttgcta tagatttctc tatacatgaa gcaaaacgtt ctgagtcaag gcgagttgtt
     2881 tatgcagatg cgtctataag tgaaaagata aagaagaaac atgttattaa aaattgttta
     2941 aaagaggcat taaaaaatga tgggtttgaa gtgtactacc aacctatatt ttctaatagc
     3001 aggggcaggt ttacttcagc agaggcatta cttaggctag aacacaaaga gttaggtttt
     3061 atacctcctg atgaatttat tccaattgca gaacaaacag gattgattaa ctcaattgga
     3121 ttagtagtct ttgagaaggt atgtagattt atagctagtg atgagtttga agatttaaaa
     3181 ttggataata tagctgtgaa tctttctgta gtacaatgta tgcaaaaaaa cttagctgat
     3241 gatttacttt atcttatgaa taaatataat gtatcaccaa ctaaatttaa acttgaaatt
     3301 actgagactg tggctatggg ctcttttaat ataattaaag aaacaatgga aagattaatt
     3361 gatttaggtg ttaagtttgc acttgatgat tttggaattg cttattctgg agttactaat
     3421 atgctttctt taccattttc agtaattaag ttagataaga gtttgatttg gtctatgaat
     3481 gaagatagta ggcataaact tacagttgag acgattatag cacttataaa taaattgaat
     3541 atgaaggctg tagcagaagg tgttgaaacc attaagtgtg ctgaagattt aatagctatg
     3601 gagtgtgaat atttgcaagg gtattatttt tctaaaccag ttccagaaga tgtttttaaa
     3661 aatttgttga aagaaaatac ttttacaagt taacaccctt tattttttta attacaactt
     3721 tgttcattga cactgccagc gatatattat aaaattaaca caataataaa tattaaattt
     3781 attatacata ttattaatat taattaatgg agtaaagatt tattatatat tttatttaat
     3841 tatcctaaag tgagaaagga agatgtgaca tgaataaaaa gaaaatatta gtgccaattg
     3901 atgggacaga aagaagtatg cattctttgg aatttataaa aggaatattt aaaaaagatg
     3961 aagttgaagt agaaataatg aatgtaaaag aactagtatt tattgatggc atatccctgg
     4021 ctgaagagat aaaaaattct gaaaatttag gaagaagaat cttggataaa gcagcagaga
     4081 taatgggaga gtatgatgtt aaagtacatt ttacttttgg atatccagga gatgagataa
     4141 ttagaaaagc aaaagaagat aatgtagatt ttatagttat gacaaaatct actaaaaaag
     4201 gtcttacaag aatgataggt tcagtaacag caagtgtagt taaacaagca aaatgtatag
     4261 tgatgattgt accggagtag aattagaaag gtatttgaaa tatgaataaa aagatagtag
     4321 cagtttgtga aagtgaaaga agtttaaatt ctcttaagac agctgcaaat gatttaggat
     4381 ttgatttagt ttatgaaatt caagaaaatg ataatataaa aaatgaactt tctatttatg
     4441 atatagaaga tgcatccata gtgttatttg ttacaagtga ttctatagaa agcatagaga
     4501 agattgatag atttatagat agagaatttt atgaagttga tccaaagtat ataatagagg
     4561 atgcaaaatc tatagtaagt gaaattttaa tagacttaaa ttaatcgtgg gtgtcgcact
     4621 aagaatagag cgacgccttt ttatatattt tacacaagaa ataaagaggt tgtatcaaaa
     4681 tatagataag actctttttc attgttgaaa tgatgaaaat atatttgatt tcatacaaaa
     4741 aagagtacct cataactaaa aagttcactt tgagacactc catttttata tgttagttgc
     4801 atttaaggta aaatagaagt ttaatcatgt tgaaataact gaattacatc ttctaaatga
     4861 ttgtacattg atgtataacc taagtaagag tctttactat caaatgcaat cattatcttg
     4921 ttatggtggc taatcatatc cttgagagaa tttctatatt taaaaccgaa gtattggtta
     4981 gaagaaactg ttttaggtat taactcataa tattttataa aaattggatt atgagaagct
     5041 aaaattatct ctttgtgaaa ttgcaagtca gcatcataac attcttctat caaaccatga
     5101 gaaaccttat ccaattcctc tattgacttt aggtaagtat attttatttt ttgaagttct
     5161 ttagatgtag catttaaaat tgctagttta gtagcttcgc tttcgataag ttgtcttgct
     5221 cttaaagatt ctacataaaa ttcttgtatg ttatgatttt tatagatttc accatcatca
     5281 aataggtcaa cttttttaaa tccttcaact ataggacttg aataaggcac agctctaagt
     5341 attccttcct tacagagtat atttatggca tttcttacag gtattctact tatgttaaat
     5401 aggtcagcaa tctttctctc acttggtaat ttactattaa ttttaaaagt tccatttaga
     5461 atgtttaatt taatataatc aataattttt tcagtggcat ttaagctttt cataaaaact
     5521 cctaaacata taattttttt atgattgtat tatatcatag ttaagagtta aatctacttt
     5581 tttataatat gtataagaca ggatagaggc caaaatagct ataatagcca agataaagta
     5641 tccatattgt attgaagtat attccgatat tttaccaact atcattggaa atacagacat
     5701 tcctatagca taaattgctt gaaaaaaacc cattgcagta gatttctgat gttttggtac
     5761 atttttcata gactcgctag taaggtaaga aaggagtata cctgtagata tacctggtag
     5821 tacttgtagt acaaatatta tattaatatt ttttattgtt ggaactaaaa agcaataggc
     5881 tgaaactaaa ataaaaacta aaggaatcca aaattttggc ccatacttag tacaaaattt
     5941 agtagaagca aagccagaag aagcaacagc acacaccatg taaaatatag aagatagacc
     6001 tatgagggta gaactagaac ctaagttttt aagaatttga gtagtaaatg acattgttgt
     6061 agacatttga ataccctgtt gaattaatgc cataaaggaa aaaaatacaa gtcttttatc
     6121 cttgcagata gataaatatt ttttaattga tattttttca ggtatttttt ttgaattatg
     6181 attttttata tttaaggcta gaatcgttcc aataactgaa gacaatacac tcatgataca
     6241 taaggctgtc attcctaatt tttcataaaa taaagtactc ataacaaatc caatcaacat
     6301 tcctagattg tttaccaata ttatcgtact tgtagctttt tgttgatgtt ctgtagaaaa
     6361 aaagttggta tagtgaacca tgtaagaaat ccaagttgat gatgatattc cagataaaat
     6421 gttagctata agaaaaccat tgcctccagg agaaaagact cttataagag atgctaaacc
     6481 agcaaagaaa attccagaca gtataaatat tttatgttta tttatggaat cagcaaatac
     6541 tccaacagga agccttaata aacattgtga gattccatat gcacctatta ttataccaat
     6601 catatttgta gacacattac atgcacttaa atatggattt tggaaaggta tatatacata
     6661 ttgtgaaaac cagaaaaatg taacaataga aagtaaaagt aatttttgat tagatatttt
     6721 tttcatatta tcctccgtat ggtattttta taatgcaaat aatataccat ttggattttt
     6781 taatcaagta ctttctctat taaaaaaaat ttaaatatat aattattatt tacaaaaaaa
     6841 ttatacttta tagttgaatt aagaaaacgc ttatgatact ataaagttaa caaatttgaa
     6901 tatataacag tagacgtgtt actggtaatg caggcaagat cgaatcaatt tttatagatt
     6961 atctatagag tactatgggt ataatctatt tatttggttc gatttttttg tttaattggc
     7021 cataaaacaa aaagcctata gttgaaaatg gaggattttt tatggattat aaaaatatag
     7081 ctcaagaaat tctactaaat gtaggaggaa aagagaatgt aaatgaagtg actcattgta
     7141 tgacaagact tagatttaaa gtcaaaagcg cttctaaagt gaataaggat aaattatcta
     7201 aaactgaagg agtaataact gttgttgaga gtatgggtca aatacaagtt gtaattggaa
     7261 ataaagtaaa aaaagtatat gatgaggtta taaaaattgt acctcaatca aataacgtag
     7321 gtaaaaagaa tgaaagtcaa gaaaagcaag gtataattaa ttctatatta tcagcagtag
     7381 ctggtatatt tacacctaca atacctgcta tagcaggggt tggtatgatt aaaggtattt
     7441 tatcagtttt tgctatgtat tatatgaata aaaatggagt agatataaaa gaaacgcaaa
     7501 gctatattat cttaaatgct atggctgatt ctatattcta ttttatgcct attatactag
     7561 gatatactgc ggctaaagta tttaatgcaa ataaaataat atctatggta ctaggagcta
     7621 cactttgtta tccaactttt actgctttga tggcaggtga agaatctgtt agatttttag
     7681 gattggcagt aacaaaagct acgtatacat catctgttat tccaataatt attgctatat
     7741 gggctctttc atacgttgaa aaagtattag aaaagtatat acctgaggta attaaaataa
     7801 taatggttcc aactttatca ttggttgtaa tgttacctgc aacattattt ttatttggac
     7861 caatcggaat ttatatagga aatgtcataa acttttctta taagtatatt tatgaattaa
     7921 gcccagcatt gtgtggagca tttgtaggtt ggttatggtg tgtgttggtt atatttggtg
     7981 ctcatagagc attattacca ataggtataa gtgatgttgc tcaaacagga agacaaaatt
     8041 tattggcatt tgcaggagct gcaaactttt cacaagcagg ggctgctcta ggagtattct
     8101 ttaaaactaa aaatcaagga ctaaaaacaa tttctatgtc tgcaactata acagctttat
     8161 ttggtataac tgaacctgca atatacggtg ccaatcttag attaaaaaaa cctatggtat
     8221 gtgccgtaat atgtggtgca tttggaggtg cgataatggg aatgggtgga gcttatggta
     8281 atgcatttgc aaaccaaggt gttttaacta tacctgtata tgctgaagca ggtgcattag
     8341 gtttcttaag ttatctaggt ggttgtgcta tagcattttt tggttcagct atttcaactt
     8401 atctagttgg atttgaagat ttggaagagt cttctaataa ggaaaattca tctataaaag
     8461 tagaaacgaa ggatggtgtt attgatataa cttcacctgt agagggcgaa tgtatagaat
     8521 tgtcagaagt aaaggatgat gtatttgctt caaaagctat gggagaaggt atagctgtgt
     8581 tacctacaaa aggagtaata acagccccta ctgattgtga agtagcttct ttatttccaa
     8641 cattacatgc aataggatta aaattagata atggagcaga aatgttaata catgttggaa
     8701 tcaatacagt agagttaaat ggtaaatact ttacaaaaca tgttaatcaa ggagatttag
     8761 tgaaaaaagg tgataaactt atatcctttg atatagataa aataaaaaag gctggttatg
     8821 atgtaactac gccagttata gtaaacaata catttgattt tgggcaagta gtttcttgta
     8881 aatctagtta tgtttcaacc aatgataata taatttcatt agtattagct ggtaattaag
     8941 ttaatttgac tagagataag taaattgatt taaaaggaaa taagtaaatc aacaattaaa
     9001 tatatctaaa agtaagttta ttgcaatgaa ttgttaaaga aaataattaa tatggagtaa
     9061 agctattgca atgaagtaac aaagaaaata attaatatag agtaagttta ttgtaatgaa
     9121 ttgttaaaga aaataattaa tatagggtaa atctatctca atgaaataac aaagaaaaga
     9181 gttaatatag agtgaactta ttgcaatgaa tcaacaaaga aaataatcaa tataaaaagg
     9241 agaaatattt atgaatacag gatttccaaa agacttttta tggggagcat catcttctgc
     9301 atttcaagtg gaaggagctt gggataaaga caataaagga aaaaccgtag ccgactataa
     9361 ttcttttaaa aagtctcatt tacaggcaga tactaaagtc gcatcagact tttaccataa
     9421 ttatgaagaa gatattgaat taatgaaaga attagggatg aaaacatatc gtttttcaat
     9481 ttcatgggca agaataatac cagatggtga aggagagata aaccagaaag gtttagaatt
     9541 ttataataag ataatagaca aactaataga gtgtgatatt gagccattcg taactttata
     9601 ccattttgac cttccattta aattagtaga gaaatataat ggttgggaaa gtagagaaac
     9661 tgtctatgct tttgaaagat ttgctaaaat atgctttaag cattttggag atagggtaaa
     9721 gtattggcaa cctcataatg aacagaattt aattgttaga gtggaggaga gaattaatat
     9781 atatgatgaa acagattcat ggaagataga taaaatgcgt gctcagatgg attataatct
     9841 ttgcttagca catgcattag cagtcaatgc atgtcatgag atgataaaag aaagtaaaat
     9901 tggagtagca gtatcatcat ctgtaacata tcctcttact tctaagccag aagatgtata
     9961 tgcagctaga atgaatgata attttaaagt gtactatatg cttgatatgc atcattatgg
    10021 agaatatcca gggtattata tgaagtatct tgaaaaaaga aatatagttc ctcacatgga
    10081 agatggggat aaagagatac ttaaaaaagc taaaatggat tttatagctg taaattatta
    10141 taggacaaat tgtgcagaag ctttacctga agattcacaa catccatttg gattaagaga
    10201 aggaactgtt gattttagta tgtatggact atttaaaatg tcaatgaatc ctaacttaga
    10261 ggcttctgag tatggagcag caatagaccc atcaggtctt agagttgcct taaatgaata
    10321 ttggcaaaga tatcacttac ctgttataat aacagaaaat ggtctaggag ctaaagatat
    10381 attagaagat ggaaaaattc atgatgacta tcgtatagac tatttaagaa gtcatataaa
    10441 tgcatgtaaa ctagctattg aagatggtgt agagatgatt ggatattgtc catggtcatt
    10501 tacagacctt ttaagttctt ctcaaggttt taataagcgt tatggacttg tttatataaa
    10561 tagaactgac catgaagttt tagatttaaa aagaatcaag aaagatagtt tttactggta
    10621 taaagaagtt atagagaaca atggaatagt aaaataaaca taaatatata ttgagggttt
    10681 tagtatgtac attacaaagg tattaaataa ttcgttgcta cttgcaaagg atgataatga
    10741 tgaagaaata atattgatgg ggaaaggcat tggccataat tataaggccg ggtctgagtt
    10801 aaataaaaaa gatatagaaa agatttttgt tcttcatgat gaaaatataa aaaagagtat
    10861 tatacaattg gcaacagaaa tagatgcaga atattttggt atagctcaaa tgataattgc
    10921 atatggaata gaaaaatata acttaaaact tatgaaccat atatatttgg ccttaactga
    10981 ccacattgcc tttgcagtga gacgttttaa agaaggtata atgattgaaa atcactattt
    11041 gttcgaaatc aaggaattta atccaaaaga gtatgatata ggaaaatatg ctattactgt
    11101 ttttaaggag gttcttggtt tagacctacc agaagaagag attggtaata tagcaggcca
    11161 ttttataaat gcacaacaag ataatcctta tagtgataga aataagagaa gtgctaaaat
    11221 tgtaaatgct attttgcaaa tagttcatta tcacttttct attgtatata ataaagagtc
    11281 attttattat aggagatttg taatgcatct aaaggccttt tcacaaagat ttctggctaa
    11341 tgaaccatca agagaaaaaa tagattttat atatgagcaa gttcaaaaaa attgtaagga
    11401 agagtatgaa tgtgtgaaaa aggtaggagc ttatatgtat aaggaatact ccagagaatt
    11461 accaagacaa gaggagttat atttgatgat tcatattcat aagattttag gagaattatg
    11521 cgaaaatagt tagaatatct taatatgcta gtaatattat aaaaaattaa aaattaacta
    11581 cactttaaat atgaataatt gcattactta agggggttgt cttaaaattg atttttttaa
    11641 ttaaatttga agctcaactc cttttttaaa cctttatatc attacaactt taatgtttat
    11701 acgcctgtac ttcagatata tatctacttt ttacatttct caacatgcaa atctaccatt
    11761 ttttgcagta tgttaattga aataaacctt tgagttaacc ctaaatttat tctggactta
    11821 taggaatagt tccaaagata ctttaaaata ttttctggaa tgattgctta tttcacatat
    11881 gttttattgt gatgttttga tttaataaat tcttcaactc tgtatatagt aaatgtatta
    11941 tcatgtttgt tttattcaag cacacttata atacgcttga ttttttcaca atgatgaaat
    12001 atactaatgg gtgttatagg tataagaata gcattacact tgaagaagca agtgataaat
    12061 gaggtataaa tcctcatatg ataaactact atttcattga ggagcatatt ctaaatgatg
    12121 taaagatact acaatatgac taaattccta gtgataaaaa agtctacgaa tgagtgtaga
    12181 aaggggaact aagatgaata aagttttaat tatagatgat gataaagatc tttgtatttt
    12241 gttaaaaaag agtatctcaa ttgaacagat tgaagcaaac tattgccatt ctgggagaga
    12301 tggactggtt gaactttcaa aacaagatta tcagttagta gttctagacg tgatgatgcc
    12361 aggtatggat ggttttgaaa ctatggaaca aatacgaaaa tatagcagta ttcccatctt
    12421 aatgctgaca tcaaaaagcg ataattacag caaagtgcat ggattgcgag ctggtgcaga
    12481 tgactatcta accaaaccat ttgaaattac agaatttact gctcgtgtac tgtctttaat
    12541 ccgacgttac acccgtttta atccaaaaaa tgaggaacca cagcaacttt ctttcaaagg
    12601 tttgttgatt gaccttgacc agcatacagt gactaccaat ggcgaaccta tgttgcttcc
    12661 acccaaggaa tttggtgtac ttctttactg tgcaaggcat cagggtaaga ttttgacaaa
    12721 acagcagatt tatgaagccg tgtggggtga gccatatgta tatgatgaca gcaatatcat
    12781 ggctattatc agtcgcctaa gaaaaaaaat tgaactagac ccatcatctc ccatctattt
    12841 gcaaacaata aaaggaattg ggtatcaatt caacacggag gtatgaaaat ggatgaacaa
    12901 acgataattc ttttcttact cttgctgtcg attggtctac tttttctttc catatttgct
    12961 ttttccaagc ttgggcatat ttacaagcgc ctaaaagata tagaggagat actggctgat
    13021 gtacaaattg gagacgaaaa tcgtaaaata ctaattaagc catgtgatgt tatggctcca
    13081 ttggtttatc agcttaatga gattatctac gattatgaaa ataagttact ttctctgaaa
    13141 aagtcggata aagctagcaa acaacttatg acaagtttat ctcatgatgt tcgtacacca
    13201 ttaacaactc tgattggtta tctggatgct gtacacagtg gtatagttat cgaacaagag
    13261 cgggaggaat gccttgaaat cgctaggcgt agagcctacg acctcaagga ttacattgat
    13321 gttctttttg actggtttcg gttgaactct gatgaattta cgctgtcaat agaatctgtg
    13381 gaaattgcag agttgtcacg taatattttg aaagactgga ttcctatatt ccatgaaaag
    13441 aaactgaatt ttgaaattga tattccagaa aatcgactaa tggtaaacct cgacccagat
    13501 ggatattccc gtgtggtcaa taatctggtt caaaatgttc ttgctcacag taaggctcgg
    13561 caaatcaaga ttaccatgag tgaggattcc agaatggtat tactccgtgt tgaggatgat
    13621 ggtgttggga ttgccagaga aaatttacca catatttttg agcgactgta taagtgtgat
    13681 aaaggacgtt cagaaaaagg aagtggtctt ggtctatcta ttgtcagtca aatggtggag
    13741 cgaatgggtg gtcaggtttc cgtagaaagt gagattggaa agtataccgt atttacagtg
    13801 agcttaccat taatataaaa ttgtttttcc cccatgtgac atgtatacac agggggattt
    13861 tttgtaagtt ataaagttgc aaggttaata caaggttgtt gcaaggttaa tgtaagattg
    13921 aggtggtatg ataactttaa taaacaaagg aggcttttat tatttatgaa taatttaatt
    13981 attgaaacaa aaaatctcac taagcaatat ggtgctcaaa agagcgtttc taatttgaat
    14041 attcacgttc agaaaggtcg catttacgga ttgctaggaa gaaacggtgc gggtaagacg
    14101 acaactatga aaatgttatt gggtctaaca aaaccaacta ctggagaaat taaaatattt
    14161 ggtcaggaca ttcacaataa cgagaagaag cttcttcctc gaattggatg tttgattgaa
    14221 tcaccagggt tttatcctaa tctgacagga acagaaaatc tcaaaatctt tgcaaggctt
    14281 agaggaatac caaaaaaaaa tgcagtagaa tcagcactgg atgtagtcgg tttgccaaac
    14341 aatgacaaaa agttattttc tcaatactct cttggtatga aacagaggtt ggcgattgct
    14401 cttgccatta tgcatgaccc acaactgttg attttggacg agcctatcaa tggacttgat
    14461 cctattggta ttgcagaagt aagaacgttt attcgtaatc tctgcacaga acgaggtaaa
    14521 actattttga tttctagtca cattctttct gaaattgcct tgttggcaga tgacattggc
    14581 attattgacc atggtatttt gttggaggaa gaaagtcttg cccaattaga agaaaaaaac
    14641 agtaaataca tacattttgc tgtatctgat gcagcacagg cagcaaggct tctggaaacc
    14701 caacttaacc tgaaaaattt caaagtagca gataattaca atattatgct atatgataca
    14761 gatgtttctg ttgcagaaat aaatcgtgct ttcattctaa acaacattga ggtatcagaa
    14821 tctcatttat gcgaagatac tcttgaagat tacttcaaga aagtaacagg ggggtgaagg
    14881 aattgcttga tataatttca tgtgaatttt caaaactcaa gcggttaaaa tttatactga
    14941 tttctatttt aggtgcttgc ttatttccaa ttcccgcaac aattttgatt gcaaaggaca
    15001 acttgccctt tgagcaattg ttcaaattag ttgtcaattt tggatatttt ttgctattac
    15061 caattgtatt aagtattgtt gctagccaac tattttttat agaaagagat aatgatgtat
    15121 taaaaaatct tgcaacagtg ccagtgccaa aaggaaaact ggctctggca aaattggctg
    15181 tgttgctgtt tatatcattg ttctactccg ttgcagggtt aggagctact atcattggag
    15241 gtttcattgt tggaatagta gagggggttg ctgtaaaatt aggaatgagc attgcttttg
    15301 gaattatgtt ttttgttact gtacttcctg ttgtggtact cattgtatat ttcaatcgca
    15361 gctatatttt ttctattata cttttttttg tatatgctat tttcaatttt agtatatcct
    15421 taaatattat caattttgaa ccaaataacc caatattaag cgtattacct gctcctgtta
    15481 taatgcgttg gtggatggca ttttggggag atcccactgg cgaatacacg gcacttagac
    15541 agccatattt gctctcaact cctgcttgtg taggtatttt atgtttgata accattatag
    15601 cagtcctgct gatttgtatg atatacaaaa agcaggagaa ttaggaggta tctatgcttg
    15661 atataattac cacagaattt caaaaaataa aaagatataa tattctatgg attggtgttg
    15721 ttgctgttct gttttcagca ctattggcag tttttcaggt tgtaagttca cacggcagtg
    15781 accctctaac gtatgagagc tttgcaaacg gagttatctg gaataatttc agtcttagtt
    15841 ttccatttgg gattacactt attggaggat atcttattaa ccgtgaatat acagaccaaa
    15901 ccttaaaaaa catactaact gttcctattt cattacgcaa attgctagtg ggaaaattaa
    15961 ttgcggttgg aggaattact gtattgttaa gtcttttcag cttcttatgc acatttattt
    16021 tggggttgtt attttgcaat attaatatat ctgttacact cgttgtaaaa tccttagttc
    16081 agattttgtc tgttaatctg tattgttatg ttgctgtttt gccgattatt gcttattttg
    16141 gtcgtaaaca aaatgcattt ctgacgggtg tagggattgc atttgtatac ggtttttgtg
    16201 gtgtgtttgt tgctggaaga aatctgacag acttttatcc aatcacagca ggtcttggtc
    16261 tagttggata caacaatggt gctggtactg tttatcagcc acttattggt gctatgactt
    16321 tgattgtaat tctaatcttg acaactattc tacttgtatt tacacctaat tatgataaag
    16381 ttatgaccat atctaagaaa aaagggataa agcataaaaa gtattctcat aaataacata
    16441 aatattgttt ctattattta agttgctaga cagtgaaaag aaaaaatctg tcgtacagtg
    16501 gctatatgct catgtattca agaagctaca gttttgattt ttcataactg tagcttcttt
    16561 ttgtatttat taaatcacgg ctatcataat acttgcaata ggtatagcga caagagagag
    16621 gactgtcata tgtgttttat atattacatt aagtatattc taaaatatat gttttaaata
    16681 atatatttga aaataggaat atttaaataa aaaaaataat attatatata gttgacaaaa
    16741 attggcaaat gagataatat ataaatatag aaatagtaat cattaccatt attaaaaagg
    16801 gaggaattaa ttatgaactt aaaaggaact aaaacagaaa agaatctaat ggcaggattt
    16861 gcaggagagt cagaagcaag aaataaatat acatactacg cttcaaaagc aagaaaagaa
    16921 ggttataatc aaatagctgc tatatttgaa gaaactgcta acaatgaaaa ggaacatgct
    16981 aaattatggt ttaaactttt acatgatggt atgccttcaa cagaggaaaa tttaaaggat
    17041 gcagcagcag gtgaaaacta tgaatggact gatatgtatg caaaatttgc taaagaagct
    17101 agagaagaag gatttgacaa gatagcttat ttatttgaag ctgttggaaa aatagagaaa
    17161 gagcatgaag aaagatactt aaagctttta gaaaacttaa atgaaggaaa aatatttaaa
    17221 agagatgaag aagttgtttg gcaatgtcaa aactgtggtc atgtgtatgt aggtactgaa
    17281 gcaccagaaa aatgcccagt atgtgaccat ccaaaagcgt atttcaatat aaaagctgaa
    17341 aattattaaa aattatcatt attaaaaagg aatgttaact atgaaatttt ctaaacaacg
    17401 agaactgatt ttaaatgaaa tattaaataa tccggttcat cctactgcgg attacctata
    17461 tgaaaactta aaaaaagata atccaaattt aagcttagga actgtgtata gaaatttagc
    17521 tcagctaacg gaacatggct ttataagaaa ggttagtatt ccaggatatc cagatagatt
    17581 tgatggtcga atagataatc actatcatat catatgtgaa gtatgtggag aggtatacga
    17641 tttagaatcc gaagttctta ataatttaca agaattaata tctgaggaga cagacataaa
    17701 aataacatct tataacataa gttttaaagg gatttgtaat aattgtaaaa ggtgtagcca
    17761 agtaggttag ttttcaaatt ctaaaaataa ttaatgaggt gagtttatgt gtagtgaaca
    17821 aaaatttttt atatgtgaga cttgtggaaa ccttgtagga atgatacaaa gtggtggtgt
    17881 acctattttc tgttgtggaa aacctatgaa ggaacttgtt cctaatacaa cagatgcagc
    17941 agttgaaaaa catgtacctg taattgaagt ggatggaaat aatgttactg ttaaagtaag
    18001 tagcacaact catccaatga ctaaagaaca tcatatagca tgggtgtatc ttatgactga
    18061 acaaggtggt caacgcaaat gtctagcagt agatggagaa cctgtagtta aatttgcttt
    18121 gaatgatgat gataaagtga tttctgccta tgcttactgt aatttacatg gattatggaa
    18181 ggctgaactg taatatttaa ataaattgag tcaaaaagga ggctataaat gtattttcat
    18241 acaaatagcc tcctataaat ttatataaat tcaagtttta attttgttta atgtcaatat
    18301 aatataagga gaaaataata tgtcaattta taaatgtagt gtttgtggat atatttatga
    18361 tgaatctaag aatgataaaa catgggatga attaagtgaa gattgggaat gccctgtatg
    18421 tacaaaaggt cgtagttatt ttggaaagat aagtactgtt tactatgaag aagatgaaaa
    18481 gatagcagaa gacatagttg aagatgaatc taaattaaat actgaaaaag agggagattt
    18541 aaattactta agtacatatt taagaagaga tgatgaagtt gagaaacata tggatataat
    18601 tcacgaaatg gctgttacag gaaaatctat aatagaacct atgagaacta aactaccagt
    18661 aatttcttgg gatgatattt taattatggg tgctcaatta aatccattac ctttaaatga
    18721 gcatgatgaa gtaaatacaa caacaataat aggaaaaaaa gcaaagaaac ctatgattat
    18781 agaaaatcca gtttatattt cccacatgtc atttggagct ttgtctaaag aactaaaaat
    18841 agctttagca aagggtgcag cccaaaataa gacagctatg tgtagtggtg aaggaggtat
    18901 attaccagaa gagaaggaag cttcatacaa gtatattttt gagtatgtac caaataaata
    18961 tagtgtaact gaagaaaatc ttaaaaattc tgatgctata gagataaaaa ttggtcaagg
    19021 aacaaaacct ggtatgggag gtcatcttcc tggcgaaaaa gtaactgaag aaatagcaaa
    19081 aataagaaat aagcctgtag gtcaagatgt aataagtcca tcttgttttg aggaaataca
    19141 atccaaagaa gatttaaaga aacttgtaga tgaacttaga gaagtttcag aagggcgtcc
    19201 aataggtgtt aaaatttcag caggtcatat tgaaaaagat atggaattta tagcttatgc
    19261 taaacctgat tttgtaacta ttgatggtcg tggtggagct actggagcaa gtccaaagtt
    19321 gcttaaagat gcaacttcaa ttccaacaat atttgctctt tatcgtgcta gaaaatacat
    19381 agatacacat ggattagata tagatttggt tataacaggt gggcttcgta tatctactga
    19441 ttttgctaag gcaattgcta tgggggctga tgcagttgct attgcaagtt ctgctcttat
    19501 ggcagcggcg tgtcaacaat atcgtatatg tggttctggt aagtgtcctg taggagtagc
    19561 tactcaggat gaggaattga gaaaaagatt acatatagaa aatagtgcta atagagtggc
    19621 aaacttttta aatgtaagtt tagaagaact taagactttt gctagaattt ctggtcataa
    19681 agatatacat gatttaagtg ttgatgattt atatactgtt aactctgaaa tatcaaatta
    19741 tacaaacata cagcatgtat agattgaagt ttgaaactat tagattttag ataactataa
    19801 aagtaaatga taaatatatt ttataaaaga gagtgtctta aaatgaactt tttagttcat
    19861 aagtcactct tttttgtatg aagccaaata tatttatcat tttagcaatg aaaaagaatc
    19921 ttatctcttt attttgagat agactccttc tatttatgaa aattaaagat attaaattgt
    19981 aaaatattct gtgatgtcgt actattaaac taaagattat gtactttaaa gtaaataaaa
    20041 atttaaggag tgattattat ggaaagattg aagatacaag ttttagtcga caataatact
    20101 tatattgata gatactttgt tggagaacca gcagtaagct attatattga gattgatgga
    20161 aataggattt tgtttgactc agggtattca gatgtattca tatcaaacgc tgaaaaatta
    20221 aatatagatt taggaaattt aactcatgtt gttttttctc atgggcataa tgaccataca
    20281 agaggtatcc agtttctaga aaataggtat gatttatcta cagttgaact tatttcacat
    20341 cctaactgtt ttatacctag aaaacatgga gagaaaagca taggagctcc attttctgct
    20401 gaggaaatta aaaatatttt tatgtataat cctaaggata agccatttaa tctaagcaag
    20461 aattgtgtgt ttttaggtga aataccttct ataaatgatt ttgaaaaaag agctaaaatt
    20521 ggtaaatgta aaataggaga tttgtgggaa gacgattatg ttttagatga ttctgctata
    20581 gtttgtaaaa ctgataaagg tatttttatt gttacaggat gttctcatag tggaatttgt
    20641 aatatagtag agtatgctaa aaaggtatgt ggagatgata gagttattgg tatactaggt
    20701 ggttttcatc tttttgaatt gaataatagg ttagatagta caatacaata tttggataaa
    20761 gaagaaataa gaatgttgta cccttgtcac tgtgtttctt ttaaggtaaa agcgaagatg
    20821 aatgaaatat tgaatataaa tgaagttgga gtagggctga ccataagtat atagtatttg
    20881 aaagtatata agatttaagt tgaaggttaa aaagatattt tctatagaaa atatcttttt
    20941 tgttacgttt attttaagag aaatcaatat acttttatat gaaatattgt aaaaaaattt
    21001 atttttaatg agaatatatt ttcattaata aacaatttta ttatgtatta ataatgttag
    21061 aataaatata aataaatcaa agttacttac attacagtat attttttcac aaaatatatt
    21121 gatttattct tttaaagttt gataattcca atgaatgtaa taa
//
LOCUS       DAEMHW010000053        20751 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEMHW010000053 DAEMHW010000000
VERSION     DAEMHW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20751
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.053"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            307..3846
                     /gene="nifJ"
                     /locus_tag="KPK67_001687"
     CDS             307..3846
                     /gene="nifJ"
                     /locus_tag="KPK67_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBG2458673.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            complement(4239..5180)
                     /locus_tag="KPK67_001688"
     CDS             complement(4239..5180)
                     /locus_tag="KPK67_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBG2458674.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPDNNKPESNKPESN
                     KPEDNNNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISN
                     VATKKSQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWM
                     NSEGHRKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(5676..7010)
                     /locus_tag="KPK67_001689"
     CDS             complement(5676..7010)
                     /locus_tag="KPK67_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG2458675.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            complement(7072..7848)
                     /locus_tag="KPK67_001690"
     CDS             complement(7072..7848)
                     /locus_tag="KPK67_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG2458676.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMNVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            complement(7953..8618)
                     /locus_tag="KPK67_001691"
     CDS             complement(7953..8618)
                     /locus_tag="KPK67_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBG2458677.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            complement(8618..9274)
                     /locus_tag="KPK67_001692"
     CDS             complement(8618..9274)
                     /locus_tag="KPK67_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBG2458678.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            complement(9283..10461)
                     /locus_tag="KPK67_001693"
     CDS             complement(9283..10461)
                     /locus_tag="KPK67_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG2458679.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            10816..11718
                     /locus_tag="KPK67_001694"
     CDS             10816..11718
                     /locus_tag="KPK67_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2458680.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            complement(11934..12746)
                     /locus_tag="KPK67_001695"
     CDS             complement(11934..12746)
                     /locus_tag="KPK67_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2458681.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(12749..13708)
                     /locus_tag="KPK67_001696"
     CDS             complement(12749..13708)
                     /locus_tag="KPK67_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2458682.1"
                     /translation="MLKLILNRIKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMGKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            complement(13814..15373)
                     /locus_tag="KPK67_001697"
     CDS             complement(13814..15373)
                     /locus_tag="KPK67_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2458683.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSVLVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            15592..16569
                     /locus_tag="KPK67_001698"
     CDS             15592..16569
                     /locus_tag="KPK67_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2458684.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            16570..17541
                     /locus_tag="KPK67_001699"
     CDS             16570..17541
                     /locus_tag="KPK67_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBG2458685.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKKIKSVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            17693..19735
                     /locus_tag="KPK67_001700"
     CDS             17693..19735
                     /locus_tag="KPK67_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBG2458686.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            19996..>20751
                     /locus_tag="KPK67_001701"
     CDS             19996..>20751
                     /locus_tag="KPK67_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2458687.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVE"
BASE COUNT         7410 a         2914 c         3255 g         7172 t
ORIGIN      
        1 tttttgtact aatataaaca aatagataaa aaagtttgaa aagtctaact taatttatta
       61 tatattgtat acaaaattat gaaaaaaact taacatattt aattttaaaa aaaatctact
      121 taaatatgta attaatttac aattttcaaa ggaattttat ctaatctgta gtattatatt
      181 agaagataga gtatttgcgc gactatatgt gtgtttatat ttttgttgtg taaattatat
      241 gtaaatataa aaaaatagac ttgttataaa caagtacaat tgtagctatc aaggaggata
      301 catattatgg ctaagtttat gaaaacactt gatggaaata cagctgcagc tcatgttgcc
      361 tatgcgttta cagatgtagc agctatatat ccaatcacac catcttcaac tatggctgaa
      421 gtagttgatg agtgggcatc acaaggaaga aaaaatatat ttggacaaac agttaatgtt
      481 gttgaaatgc agtcagaagc aggtgcatca ggaacattcc acggttcttt acaaggagga
      541 gctttaactt ctacttttac agcatctcaa ggtttattat taatgatacc taacatgtat
      601 aaagttgcag gagagttatt accaggagta ttccatgtaa gtgctcgtgc tttagcatct
      661 caagcgttat caatatttgg ggatcaccaa gacgttatgg ctgctagaca aactggatgt
      721 gtattattag cttctggttc agttcaagaa gtagctgata tagcacctgt tgcacattta
      781 gctgcaatag aaggtagatt accatttata catttctttg atggattcag aacttcacat
      841 gaaatccaaa aagttgaatt actagaaaat gaagattatg caagtttatt aaactttgaa
      901 gcagttcaag catttagaga taatgcttta tctccaaatc atccagttgc tcgtggtact
      961 gctcaaaacc cagatatata tttccaaact agagaagctt ctaataaata ctatcaaaat
     1021 atagtaggta tagttgaaaa atacatggaa aaaatgagta atttaacagg tagaaaacat
     1081 agtttatttg attactatgg agcagaagat gctaaatacg tattaatagc tatgggttca
     1141 gtaacagaaa caatagaaga aactatagat tacttaaatg ctaagggaga aaaatatggt
     1201 ttagttaaag ttcatttata tagaccattc tcaatgaaac atttcttaga tgctatgcca
     1261 tctactgttg aaagaatatg tgtacttgat agaactaaag agccaggttc aactggtgaa
     1321 ccattatact tagatgtacg tgatgtattc tatggaaaag aaaatgctcc tatgataata
     1381 ggtggaagat acggattagg ttcaaaagat actactcctt cagatataaa aactgttttt
     1441 gataacttag tttcagaaca acctaaaaat ggatttacag taggtatagt tgatgatgta
     1501 actaatactt ctttaactcc atctgagcca ataaaaatag cttctaaagg aactataaga
     1561 tgtaagttct ggggattagg ttctgacggt actgttggag ctaataaaca agctatcaaa
     1621 ataattggtg accatactga aaaatatgct caagcttact ttgattatga ctctaaaaaa
     1681 tctggtggta taactatgtc tcacttaaga tttggtgata ctccaataag agctacttat
     1741 ttaatagatg aagctgatta tattgcctgt cataaacaat catatgttta tcaatatgat
     1801 ttattaaaag gtcttaaaaa aggtggtaca tttgtactta acactatatg ggaccaagca
     1861 ggacttgaag aacatttacc agctcatatg aaacaatata tagctaaaaa tgatataaaa
     1921 ttctatacag ttaacgctgt taaactagga caagaaatag gtcttggaaa cagaataaac
     1981 atgataatgc aatctgcatt ctttaaatta gctgaaataa taccagaaga agatgctgtt
     2041 aaatacttaa aagattctat agttaaagct tatggtaaaa aaggtgaaaa aatagttaat
     2101 atgaactatg ctgctgttga tgcaggaata aatgctttag ttaaagttga agttccagct
     2161 tcttgggaaa atgctgttga tgcagataaa ggagaagtta aagaaccaga atttataaaa
     2221 aatatactaa gaccaatgac tgcacaagaa ggaaacaact taccagtaag tacattcaat
     2281 ggtatagaag atggaacatt cccttgtggt actgcagctt atgaaaaacg tggtatagct
     2341 gttgatgttc ctgaatggat aatagataac tgtattcaat gtaaccaatg ttcttttata
     2401 tgtcctcatg catgtataag accagtatta gttactgaag aagaattagc taatgctcca
     2461 gaaggatttg aagctaaaaa agcattaggt aaaggattag aaggattaaa atatagaatt
     2521 caagttagtc cacttgactg tacaggatgt ggaaattgtg ctgacatatg tccagctaaa
     2581 gaaaaagctt tagttatgaa gccaatagat actcaacttg atacagagtt agataattgg
     2641 gcatttgcag taaatcctga tgaagtagct ccaaaagttg atgttatgcc tgcaaatact
     2701 gttaaaggaa gtcaatttag acaaccatta atggagtttt caggagcatg tgctggatgt
     2761 ggagaaactg cgtacattaa agtagttaca caattatatg gagatagaat gatgatagct
     2821 aatgctactg gatgttcttc tatatgggga gcttctgcac catcaattcc ttatacttgt
     2881 aaccatgaag gtaaaggtcc atcttgggca aattctttat tcgaggataa tgctgaatat
     2941 ggatttggta tgtacacagc tgttaagcaa ataagaaata aaatagtaga tgctatgaca
     3001 gagttagttt ctatggatat ctgtgaagat gctaaagcag tatttacaga gtggttagat
     3061 tctagaaatg atggagaagc ttctaaagta gcaagtgcta aggttgttga attattagaa
     3121 aaaccagctt gtgattgtac agatgaaaaa gctaaagaat tagtaaaagc tataaaagat
     3181 agaaaagatt atctagttaa gagatctcaa tggatactag gtggagacgg ttgggcatat
     3241 gatatcggtt atggtggatt agaccatgtt cttgcatctg gtgaagatgt aaacgtactt
     3301 gtatttgata cagaagtata ttcaaataca ggaggtcaag cttctaaagc tactccagct
     3361 gctgctatgg ctaagtttgc tgcttcaggt aaaaaatcta gaaagaaaga tttaggaatg
     3421 atggctatga catatggaaa cgtatatgta gctcaagttg ctatgggtgc tgataaaaat
     3481 caatttatca aagccgtttt agaagctgaa aaacatgatg ggccatctct aataatagct
     3541 tatgctccat gtataaacca tggattaaaa gaaggtatgg gaagaactgt agctaacgaa
     3601 gcacaagctg ttgcttgcgg atactggcat ttatacagat tcaatccaga agtaaaagct
     3661 gaaggtaaaa acccattcac tttagattca aaagaaccaa ctgcaagctt caaagatttc
     3721 atacttgcac aagtaagata ttctgctata gctaaacaat tcccagagga agctgatgga
     3781 ttatttgcta aagctgaagc tgatgctaaa gaaagatatg aaggatacaa aaaattagct
     3841 gaataatatt aaagtattat aatgcttata tagaattgat aaaagagagt gtctcaaagt
     3901 gagcttttta gttcatgagg cactcttttt gtatgaaatc aaatatattt taatcatttc
     3961 aacaatgaaa aaggccttat ctctattttg agacagcctt tttttatgaa gatattaaga
     4021 ttttgttttt acctattaaa tatctttttt aagttttcca ttttcatctt gccaatactt
     4081 tttatatatt gttttacaca ctaaaattaa gagaacaaat aatatttata gtatgtatat
     4141 ttggatttga taattttaaa atagatacaa aagagtatct catttgtgtt ttttatcact
     4201 tagagatact ctcttgatat gtatggaaaa agagtatttt atcctacaaa gatttgtgtc
     4261 caataaattg aaccaccact tttttgtgct actccaacac ctattttact gaaatttgga
     4321 ttcattatat tttttctgtg accttcagaa ttcatccatg cgtttacaac ttctttagga
     4381 gttttttgac ccatagctat attttcacct gctgtcttat agcttattcc aaattttttc
     4441 aacatgtcaa atggagaacc ataagttgga gaattgtgtg aaaaatagtt gttatcaatc
     4501 atatcttgag atttttttgt agcaacattt gatatagaag aatcaagagt aagaggattt
     4561 aatccagctt tagctctttc tatatttact aagtcaacaa cttctttttg gtaagctgaa
     4621 aaactttcac ttgtagagcc tgagttgtta ttatcatcag gtttgttagt agagtctgaa
     4681 ttattgttgt cttcaggttt attagactca ggtttattag actcaggttt attgttatca
     4741 ggtttatcag tagagcctga attattgttg tcttcaggtt tattagactc aggtttatta
     4801 ttatcaggtt tattagtaga gcctgaatta ttgttgtctt caggtttatt agactcaggt
     4861 ttattgttat caggcttatt agtagagtct gaattattgt tgtcctcagg tttattagtg
     4921 ttgttgcaat tcttgtcatt atgattctta tccttacaag agttatcttt atcaggctta
     4981 agttctggtt ttacagaaca atttggtttt gcagaaaact tgtatttagt attactatat
     5041 actaaacttt tgttacttat atttacatta ttttcaagtg atgaagcact tgcagtaaca
     5101 gcacttgaag ctgttgctaa agttaacact cctaaagcta atatagtttt taattttgtt
     5161 ggtatatttc ttctcatcat taaacacctc ttaatctaaa aattctaaaa atgttactga
     5221 tttgtaactt ttagtgtagg gggttacccg tgaatctata ctaacataag attaagactt
     5281 tttttactgt aaagatatgt aaaaaagata tatatggaat aaataggtat atttcagtgt
     5341 gtttgacaat ttgtattaaa agcctgtata gtatggaatt acttagttag agaaaataat
     5401 atttttataa taattccata acgtaaattt atagttatga taaatttttt ttatttggtt
     5461 aaatttattc agtagatgct ttcgtattag caacaaatta taattattta aatctttaag
     5521 aaataaaaag ggtctgtctc aaaatagaga taaggttctt ttttattgtt gaaatgatag
     5581 gcatatgtta tttcatataa aaagagtgtc tcattaagta aaaagttcac tttgagacac
     5641 cacctttcat attatgaatt ttaatttgtt ttatattaat acattagtaa tcctaatgta
     5701 attacaataa tttctgctat tagtgaacca actgcaacta caatgaaagt ttctttaaat
     5761 ccagttttat gtgttaactt agataagctt aagaatgtaa gaaaaacacc actttgtgga
     5821 actatagtta gtatatttga ggctattgta gcaacacggt gaattaattc tggatttaga
     5881 ccagtactta aataataatc tgcaaagttt ggcattacta ttcctaatgc accagaagag
     5941 gaccctgtta ttgctgacat tgttgaagtc aaaacagtaa gacttataag tggactacct
     6001 ggtattgaca ttataagttt gctaaataca tcaaaaccag gtgcagccat tattactgct
     6061 ccaaatgcaa ctgcagaaga tgtagcaaat ataggaccta tagaaccact agcacctatg
     6121 ctcaatgtct gtatcttaga tgatagaaat ttatggaata aaaaagcaga taaaagaatt
     6181 gcaactatta aagctatgta aattacattt tttttcataa attctgaacc aaaagtactt
     6241 ccagttatag ctataattac gagtgctaat agaggaaaaa tacttgcaaa aaatcctggt
     6301 aattctctat ctaaagattc ttcttctaat tctgaagtat aagttgcata agtttcacct
     6361 tttctaatac ttttgtttag agcatatttc atatagacaa gtccaaacgt agcacaacct
     6421 atactaccta caatacttgg tatagtagct gcagtaagag acgtatttag atattgaata
     6481 ggtataacat tttgaatggc tggtgttcca ggaattatag tcatagtaat tgttgcaata
     6541 ccaagccaaa gtggtatctg aataaggttc caagaaatat caagtttttt aaatagagtt
     6601 cttgcaagtg gaatgaccgc aaacataact acaaataagc ttataccacc atacgttaat
     6661 ataaaactta ctataaatat agcaactaaa actctataag gatgattaga gccaaattta
     6721 tttaaaataa aatcagctat tgatacagta gctccacttt cttccataaa tttagcaagt
     6781 attgagccta ataagaatat tgcaaaataa ctcattatat agtttccaag tgcacccata
     6841 taattgtttt tgtcagcacc tagcattgat gttacaacat ccattttatt taaaataaca
     6901 actattaaag ttgctattgg agcagctata gttatatgaa tttctttaac tgaaaaatat
     6961 ataatagcta tgataccaat aattatacca attggtgcga taatactcat aaatatccct
     7021 ccttagtaga aggacctgaa gaatactttc ttcaagccct aaatataata actattgtgc
     7081 agtatatcct ccatctagta agcaagcttg tcctgtaata cccttagcat tatcacttgc
     7141 taagaacata gcatagtcgg caatttcttg aacatctaat aggcgttttt gtggtacaag
     7201 tgggtataaa acttcctcta agacattttc caatggtata ttacgagttt ttgaaaggtc
     7261 attgaactga cctctgacaa gtggagtatc tacatatcca gggcataaag cattaactgt
     7321 tataccagaa tcggcagctt caagagcagc aacttttgtt agtccaataa gtccatgttt
     7381 tgctgagtta taagcagcct ttccagcaaa tcctattaca ccattaattg aggccatatt
     7441 tattatacga ccaaagcctt gttttttcat tataggaaat gcttttttag tagctataaa
     7501 aggtgctgtc agcattattt ttatcataaa ttcaaattta tcagttggga aatcttctat
     7561 catagaaaca tgttgaagtc cagcattgtt tattaatata tctaatctgc catacttttc
     7621 aacagttttg tctatagcgt ggttaatttc ttcttcctta gttacatcac actttacatt
     7681 catgcaatca taaccttttt tttgtaactc tgttatggta ttatttaaag cttcttgatt
     7741 tatatcagag aacattactt tagcaccatt ttttaagaac gattcagcta tttgtttacc
     7801 tattccactt gcagctcctg ttacgaatac aactttatct ttaaccattt tcatttcctc
     7861 caattttatt ttatttttat attaatattt taagcaatac aaatttatat gtaatacgtc
     7921 taagagattt ttattttcta gttttaatta ttttaaacaa cagatatttt tccatttaat
     7981 gaatctgaca atattaaagg agcctcagta gcagaaataa catcatctaa tgtgaaagct
     8041 ggatttattt ctttaagtaa aattccatct gatgttactt ccataacacc catttcagta
     8101 actatcaaat ctacagcatt tgttgcagta agtggaagag tacaattatt taatattttt
     8161 gcagaacctt ttgaagtatg ttccattgct acaataactt ttttagctcc agttactagg
     8221 tccatagctc cacccatacc tggaaccatc ttacctggaa tcatatagtt tgctatatta
     8281 ccatatttgt caacctgaag tgcgccaaga acagttatat caacatgacc tcctctgatt
     8341 attccaaaag aagtagcact atcaaagaaa catcctccag gaagtatagt aactggttga
     8401 ccacctgcat ttacaatagt ttcatcactt gaatttccat cttcggctga acctaaacct
     8461 aaaaagccat tttcagattg taagataacg tttacattat ccggaatata atttgctact
     8521 ttggttggta gacctattcc aaggtttaca acggctccgt cttttaattc tttagatact
     8581 ctatttgcaa tatattcttg catttctaat ttatccatta taaattacct ccttcaacga
     8641 tgtaattcac aaaaatacct ggtgtattaa cttcatttgg gtcaatatca ccaatttcaa
     8701 cgatttcatc tgcttcaact attgtaattt tggcagcaga tgccattaaa ttattaaaat
     8761 tgttcataga gcctttataa actagattac ctgctttatc gacctttgat gcaaatagaa
     8821 gagcaacatc tgcataaatt ggtttttcaa gaaggtattc tttgctatcg acaacaattt
     8881 tttgctttcc ctcctcaaca agagtaccaa taccagtcgg tgtgagtata cctcctagac
     8941 cataaccagc tgcacgtatt tgttcagcaa gagttccctg aggaactaac tcaacatctg
     9001 tttcattgtt gttcatttgt cttccagttt ctttatttaa tcctacatga gaagcaatta
     9061 tctttttaaa ttgcttattt acaaccattt tgccaactcc tttgtcaata aagccagtat
     9121 cattgcaaat tagtgtcaaa tcttttatat ttttactaca taaataatca attaaatttt
     9181 ctggtgaacc attggccatg aatccaccaa ccattattgt atctccatct tttacatgag
     9241 atagagcttc atcaatgcta actattttat tcatatttcc tcctatcgtg aaaccaccat
     9301 tgatattcct tgaccaccac caatacaaag tgttgcaagt ccagtttcaa ctttacgttt
     9361 ttgcatttca taaattaagg taacaagtat tctagctcca cttgctccta ttggatgtcc
     9421 taatgctatt gcaccaccat taacatttaa ctttgatgaa tctatttgaa gttcattttt
     9481 aacagctaat gcttgggcag caaacgcttc atttgcttct attaagtcta tatcattgat
     9541 gctaagtcca gcttttttca gtgcttttct agttgcaggg attggaccag tacccatgac
     9601 ttcaggttca acaccagctg aagcatagga ttttatttta gcaagtggtc taatacctag
     9661 ttcgtctgct ttttgttggc tcattagtat taacatagcc gcaccatcat taatacctga
     9721 ggcatttcca gctgtcacag taccatcttt tttgaatgca ggtttaagtt tagctaaacc
     9781 ttcaaatgtc atacctaatt taggatattc atctttatca atagtttcta tttttcctcg
     9841 acgtattaaa acatctactg gaacgatttc ttctttaaag cggttgtttt ttatagcatt
     9901 ctcagcctta ttttgacttt ctaatgccaa tttatcttgc atttctctag taaattcaaa
     9961 tttagttgca atattttctg cagttattcc catatgatac tgattgaaag catcaataag
    10021 gccatctgtt agcatactat ctaccatggt tatgtcaccc attttactac caaagcgagc
    10081 agttggaacg atataaggag cttggctcat attttcagta ccacctgcaa ttaccacatc
    10141 attttcacca gatgtgattg attgggcagc tagctggaca cttttaagcc ctgaaccaca
    10201 taatttattt actgtgtagc ttggaacaga atttggtata cctgcattta tagcaatttg
    10261 tctagcaaca ttctgtccaa gccctgtttg aagaacattt ccaattatta cttcatctat
    10321 ttcagataaa ttaagtccta cacgtgatat cgcttcttta acagcgatag ttccaagttg
    10381 aacagctgaa gtatttttaa atacacctcc aaaacttcct ataggtgttc tgactgctga
    10441 tacgattact acatctttca tatctaatag ttacctttct tgaatgataa ttaaaaattt
    10501 tctgaataaa gaaattaggg aacactataa taaagtactt ttttaataca agcttgtttt
    10561 taagtatttc aattactttt attcctaatg ctttaggttt tcctgtttct aattatagat
    10621 tacggcgaaa aaagcgtttt gtctaagtct aattttttat atagtgtata agaaaaattt
    10681 ataggataaa aatttatagt agatttagaa aaatattttg ttaaaatatt aatgaaatta
    10741 tgttgatttt cctataaaat atcattagaa gaaaaaatat aatctaaatt atacttttgg
    10801 ggaagggggt ttaaaatgga tataaaacag ttgaaatatt ttgttgaaat agtgaaatct
    10861 ggttttaatt tatcaatagc atctaagaca cttcacatat ctcaaccagc attaagtcaa
    10921 attataaaaa attttgaaga aagtgaagat gtatatttat ttgaaaggta taaaggcagg
    10981 ttaaatgggt taacaccagc aggagaaaga ttttatgtaa atgcagaaca tatcataaat
    11041 gaatacaaaa atatgatgga agatttaaga gaagattcag ttcaatttaa aggaaaagtt
    11101 agaataggaa tacctccact tatacttgga atagcatttt cagatgtagt tgcacagtta
    11161 gttgcaaata atccagatat agaatttgat atagtagaga agggtgcata tgacttaaat
    11221 agaatgctta tcttacagga attagattat gcagtattgt tacatccaca taaaattgat
    11281 aaaagtgtag taactgaaca tgtgcttcaa gaagatgaac ttacagcatt tttaaatata
    11341 aatcatccac ttgctaagaa ggataagata gattggaacg atttgaatga gcagttactt
    11401 gcaatattta atcctacttt tatgatacat cataagctaa tgcaaaaatt tactgatgaa
    11461 aatgttaagt taaaaagata tataatgtct ggttcatggg attttttgtt gctatcaacc
    11521 acaaattctg attttataac aattctacca tcaccagttc atgatttttt tgaaaatagc
    11581 aaaattatag aaaagccttt taatcatcct attacatgga aagttatatt atgtagacct
    11641 aaaaaagaac gttatagcca tgtagaccaa catgtattta aatttattat agatttcttt
    11701 agtaaaaaga actcttaaaa atgttattaa aataacaaat aaataaatca atttttatta
    11761 taataaacta atagtttttc atctatatgt ttgagaacaa taatacgctt tacataacat
    11821 ggtagagtag tatttaaagt tataatatac aaaagatgta tttttttaaa cgaaaaataa
    11881 aaacagtgta tatctttaga taatcactgt ttttattttc tatataaaat ttgttaagta
    11941 ttactagaaa attttttgct tattatagtc aaactcatga ctgttaaaaa tataagtaat
    12001 cctggaaata cagctaaata taacttgtca aaaatatgtc tttgagcatc ttgaagcata
    12061 ttaccccatg atgcaagagg caattggatt ccaagaccta aaaaactaag agcagattca
    12121 gttaagatag cattagcaat atttaacgaa ccagcaacta caatagtctc ttttaagttt
    12181 aaaactatat gtcttaatag tatatttcta gtcttgatac cagtagcttt agcagctaga
    12241 cagtattcat tttcctttat ttttaatgtt tcgccacgta caattcttga aacttccatc
    12301 caacttaaga atccaataat tagtataata gtggaaactt taggtgttag ataagtattt
    12361 aatacaatca aaactaagaa acttggtata ctcataagca tatctactaa tctcatcaag
    12421 aattggtcta gcttacctcc aatatatcca ctaataacac ctacagcagt accaaatact
    12481 acagaaataa gcattgataa aataccaaca ctaagtgaaa tccttccacc ataaagagct
    12541 ctagagaagt aatctctacc aagttcgtct gtaccaaaga tatgctttaa gctaggttct
    12601 tgaagtttag aagtagtatc aattgcatta gagtctatat ttaatataaa agaagctata
    12661 attaatatga ttattatgct aagagtaata catgcaaaag caaaggattt atcttttttt
    12721 aattttttta agatacttgt aaacacaact agaatcctcc tttgctagat tttattttag
    12781 ggtcaattaa taagtatagt atatcagcaa ctaaattacc taaaattaaa aatagtgctg
    12841 ataacatagt tattgccata attacagtat aatctcttga aaaaattgaa tcaatcatta
    12901 atcttcccat acctggccaa gagaaaacta cttctgtaac aaatgcacca gtgactaatt
    12961 tttgtatact catgcctagg attgtaacca atgatgtcag tgaatttttc aagacatgtt
    13021 taaatagtat gactttttct gataatcctt ttgctcttgc tgtaataaca tattgttttt
    13081 ttaattgttc atttacacta gcttctacat aatttgtaaa aatagcagta ttatatagac
    13141 ctaaagttat tacaggcaaa actaaatgtt ttactaaatc tccaatacta tcatttccaa
    13201 tagtatgcat acctcctgat gggaaaatgt taagtttaac tgaaaaaatt gaaatgagta
    13261 tcatacctat ccaaaaagaa ggtatagaaa cacctatata attaaaaaca gatgatactt
    13321 tactagttat tgaattttta tttacagcta aaaatattcc aagaggtata gctaccaata
    13381 ttgaaaatat caaagaagta cccataagaa taaatgtatt tcccattttt cccataattt
    13441 gttctgtaac gggcatatgg tttatataag aaatacctaa gtttccactt aaagtgtttt
    13501 tcaaccagtt taaatatctt atatatactg gtttatcaag ccccatatta acccttatat
    13561 tttcaatatc ctgtgaagac attaaaggat ttatatatgc atctgctggg tcaccaggag
    13621 ctaattctaa aagtccaaat gataatatag atattaagat taacataggt attaaaactt
    13681 ttattctatt caatattaat tttaacataa gtaaattctc ctaattaaaa gagatgtctc
    13741 gttttaaaat aataaaacaa tattattttt agacatctcc ttttctaatt aataatataa
    13801 aattttacat caattattct acatacagtt tagaccaatc ttcaaataat attgcaggag
    13861 atggtttagc atcttttatt ccttttagat ttttttgagc tgccattaaa ttttgttcat
    13921 aatctatagt gtaaataggc gcatccttag atatatcctg ttgtatttgc ttatatattt
    13981 cttcacgttt ttccttgttt aactcagtaa atcctttttc ccataaagca tctatttcag
    14041 cattagaata tttagtttgg ttgcaatatt gaccagtttc aaacatacct ctatatgggt
    14101 ttggttctaa tccccaagca taaccattta catacatatc acattcagtg ctattactaa
    14161 atagtatatt gaagaatgcc ttactttcat aaggaactat ttcagcttct ataccaattt
    14221 ttttcaactc ttgttgagct actaaggcat agtttttatg cccaaatcta cctgtgttat
    14281 aacctatttt taatttatca attttaacac cacttttatc caataagtct ttagccttat
    14341 ttgtatcttg gtcatatcct tcaacacctt cttctgtata gaagtctgct tctggaacta
    14401 atacagattt agcaggtaca gaaccttctt ttccatatgc tgattctatc atttcatttc
    14461 tatttaaggc atatgataag gcttgtctta cttctttgtt agccatgttt gatatatttt
    14521 ggttaaatac aatgtagttt aatctttctt cactataagt ataagtttgt aagtttgagt
    14581 ttgattttgc cttttcatat ccttcagcag atgtcttcat aagtgaaatt tcgccattat
    14641 ttaaagctgc ttcttgagat gcttcattag gaattatttt tagcgctata gaatctgcct
    14701 taggttctcc accaaaataa tctgcattct tttcaaatac tatgctttca ccttttttcc
    14761 attccttaaa cttgaatgca ccagaaccaa ctggattatt atttttttct gacttagcta
    14821 tatttgattc tccttcaaac acatgttttg gtattggaga aattttagat ataccatata
    14881 agaaagattc acttgcagtt ggtaaagtaa tcttaactgt taaatcatca attttttcta
    14941 ctttaactgg tttctcaccc accaataagt attgtcttga tggtgaattt tgtttttcgt
    15001 ctaaaactgt attaaatgta aataagacat cttcagcagt tattggtttt ccatcatgcc
    15061 attttaaatt ggattttaat tttatagtta attccttact atctttaaaa tctacttttt
    15121 ctgctaaata atttactaaa tttccttttt cataagtata aagaggagca taaactatat
    15181 ttgatgcaag agagtcttct ttaacgtttg caaaagctcc attgattata tctgggtcac
    15241 ttgccatagg tattacaaca gttccaccag atttgcttcc actagacttg tctttatcat
    15301 tggaagcaca ggctgtaaat gtaagcatta atgatgaaac taagattaat gatgccaatt
    15361 ttttaaattt catattctta taaaacctcc ataaaataat atttaacaca tattttttat
    15421 tgtataatag taatcattat taaatcaata tatatagaaa ttaactatta tattattata
    15481 attaatagaa aaaaataata aaatgtaaga aaatgttgta atttaaattt ttataaaata
    15541 caatcaaaag gtgtatgtta tcttaagtaa ttttgctgag aggagaaaat aatgagtaaa
    15601 gcactaattg aggttaaaga tttaaaagtt tattttcata cagataaagg gattgtaaag
    15661 agtgtaaatg aagtaagttt taatataaat gaaggtgaaa ctattggaat agttggagaa
    15721 tcaggatgtg gtaagtctgt aacagccatg tctttgatga agttacttcc aacatcaaaa
    15781 atagaaggtg gagaaataat ctttcgaggc aaagacattt taaagatgaa tgaagatgaa
    15841 cttatgggaa taagagggaa tgagatatca atgatttttc aagaaccaat gacatcttta
    15901 aacccagcgt ttactgttgg aagtcaaatc attgaaggta ttatgataca tcaagattta
    15961 tcaaaggaag aagctaagaa aaaggtaatc gatatgataa aactggtaga aataccaaga
    16021 gctgaagaaa tatataattc atatccacat gaattatcag gtggtatgag acaacgaata
    16081 atgatagcta tggctttatc atgtaatcca aaattactta tagctgatga accaacaaca
    16141 gctttagatg ttactattca agctcagatt ttagatataa tgaaaaatat aaaagaaaaa
    16201 ttaaacacat ctataatgat gattacacat gatttgggag tagttgcaga aatgtgtgac
    16261 aaagtattag tgatgtatgc tggcaaaatt atagaagtgg cagaggttgt tgaattattt
    16321 aaaaatccaa aacatcctta tactattgga cttctaaaat caaagcctgc tcttggaaaa
    16381 aacaaagata aaagattgta ttcgattcca ggacaagtac caaatccaat tggaatgcca
    16441 gattcatgtt attttagtga tagatgtgaa aaagtatgtg ataaatgtag aactcagata
    16501 cctcctttaa tagaattgaa tagtggtcat agtattgcat gttggttata taaaaaggag
    16561 gagatataat tgaaaaaaga agagagttta ttaaaagtag aaggtcttaa aaagtacttt
    16621 gaagttaaat ctggattatt tgataagaaa attaaatctg taaaggcagt agatggaatt
    16681 tcttttgatt taaaaaaagg agaaactttg gctatagttg gagaatcggg atgtggaaaa
    16741 tcaactgcag gaagaaccat tcttagatta attgaaaaaa ctgaaggtaa agttgaattt
    16801 aatggtgtag atatatataa gttagagaag gaagaactta gaaagttaag acccaaaatg
    16861 caaatgatat ttcaagaccc atatagttca ttaaatccaa gattaaatgt ggaacaaata
    16921 atatccgagg cagttataga acataattta gtgccaaaga atgaaataaa agataatata
    16981 ttaaatgttg tagaaagttg tggtctttct aagtaccatt taaatagaaa accaggtgag
    17041 ttttctggag gtcaaagaca gagaataggt atagcaagag cattagcatt aaatccagaa
    17101 tttatagttt gtgatgaacc tgtttctgca ctagatgttt ctattcaatc tcaaattata
    17161 aatttactta tggatttaca ggataaatat aaactttcat atttatttat atctcatgat
    17221 ttgagtgtag taaatcatat tgcacataga gtttgtgtta tgtatctagg ttctataata
    17281 gaaattgcaa ctaaaggaga actttactta cattctcaac atccatatac aaaagcgctt
    17341 atgagcgcag taccaataca agaccctaca attaagaaaa atagaattat attaaaagga
    17401 gacataccat ctccaacaaa tccaccaagt ggatgtaagt ttcatacaag atgcccttat
    17461 tgtaaggaaa tatgcaaaaa agagattcca gttttaaaag aaatatcaag aaatcatttt
    17521 gttgcatgtc atctagtata gatatttcaa aaattaaatt tgatgtatat aataagaata
    17581 atagaataaa ctgaaaattt ttttgattaa gatgggattt catgctgtta atattaagtt
    17641 agttgatata taagtttaat ctataatatt attattgaaa agagaggtag ttatgtcaat
    17701 tttagtaaca ttgttgggat tattggtttg tacaacgatt tcaactgtat ttagcaaaaa
    17761 gtggagtaat attcctcttg caatatatca aattgtacta gggattatat tgtctatttt
    17821 gccatttaaa ttctcatttt catttaatcc agaaatattt gtaatatgta tcatagctcc
    17881 attactattc agtgaaggac aaaatgtatc gagaaaagag ctattagagc tcagaaaacc
    17941 aattttgctt cttgcatttg gtttagtttt aataaccgtg tttgcaggtg gaatctttat
    18001 acattttctt attcctggga tgccattatc tgtttcattt gctttggcag cagtaatatc
    18061 tccaactgat ttagtagcag taaaatcaat tacacaagga ttaaattttc ctaaaaatat
    18121 gatgagtata cttgaaggtg aatctttatt aaatgatgct gctggagtag tagcatttaa
    18181 ggttgcagta cttgccacag ttacaggagt tttttcaata gaagaggcag ggattcaatt
    18241 tatgattaca gcttttggag gaataatagt aggaagtatt ttaggataca taattataaa
    18301 aataagactt agtttacaca agtggaattt ggaagaaatt ccaatggtaa tagttataca
    18361 aataatgact ccactttttg tatattttgt agcagaagaa ataggagtat ctggaatcct
    18421 agctgttgta atggcaggga tagctcatgg aattgaaaaa gagcatttac aaaatacaac
    18481 tactaaatta aggataatat cagacaatac ttggtatgtt ttggagtatg tattgaatgg
    18541 atttgtcttt acactgcttg gttttttgtt accaagtata tatacaggac ttagtagtaa
    18601 aaatgaaaat atggcattgg aattgacttt tatttctact ttaattgtac ttattttatt
    18661 tgttataaga tttatatggg tatatttatg gcataattct tttataaaac ctaaaaagaa
    18721 tcctttaaat aatttttttg taggtttttt aggttttaag gatgaagaag ttgtaaaaga
    18781 gagtatttct aaatgtaaat actcactaat agttgctact tgtggtgttc atggtacatt
    18841 tacactagca actgccttat ctataccttt ttatttggct gataaagatg tatttccaat
    18901 gagagatact gttttattta tatcatcaga agttattctt ataagtttag tattggcaac
    18961 agttttactg ccaagattat taaaaaataa cgtacaagag tcagaacctt tattatctga
    19021 taaagaggca tataaactaa ttttgaaaga agccatttta aaacttagta atgaaaaagc
    19081 acaagaagta caaccagtta tacatgattt aaatgaacag ttggtagact cagaaaaagg
    19141 aatactaaat actccagata ataaacttat aagtatgatg attgcagaag cagaccaaca
    19201 gggattatta gctgttttga aactattaga agatggtaaa atttctgata gagcctttaa
    19261 attgtataga ttttatatta caaaatcaag aaagtctgtt caaaaatcta tatttaaaat
    19321 tgtaaaatta aaaataagca tgtggattat aaatagaaaa gtaaagaaag aagtaaaaga
    19381 aaaaattaca aaaatagtat ctgagaataa agaaataatc aaagagtttc accatgcata
    19441 tgcactttct actaaaactg ctatatcatt tataaataaa aatactgatg aagataacag
    19501 acatgaggcc ttaatagcaa tcagattcta taatcgttat ttgaattgga ttactagaaa
    19561 gattcaaaat gatgataagt ttgaggcaag agtagaatac tacagagtaa tggcgataca
    19621 aagtcaaaga gacagtgttc aacaattagt tgaaactaag caaatatcaa gagaagtggc
    19681 taaagagtta ttggaaaata ttctctatga tgaaatgatg atgatagaat cataaatata
    19741 atggaataaa atttgatttt tgatgttaaa attaatacta aaaaatagga aatagttttc
    19801 aataatttac ttgacatata tatttattta taatagaata aaaccataaa ttgaatacaa
    19861 tatataagcg ttaacaagct ggattgttac tggtaagcag gcaataccta ggttaaggta
    19921 tacaggtgaa ttgtatctta atttaggtgt ttttttatga ataagggaaa tagtattttg
    19981 ggagagatat tgattatgat tattcaacaa atttataata ataatgttgt attagtttta
    20041 gatgaaaata taaaaaaaga attaatatta acaggatgtg gaattgggtt tcaaaaaaag
    20101 aaaggtcaag aaatagataa aagtaaaatt gaaagaactt ttgtaataca agatgaaagc
    20161 tttttagata aaattagtaa actagcaagc caggttgatg agaaattttt tgaggttagt
    20221 actgaaataa ttgcttatgc agaggagaat ttgaatacta agctttatga atatatttat
    20281 gtagcactta ctgaccatat tgcatttgca attaaaagat atcatgaaaa cataactata
    20341 aagaatgatt tactacatga gataaaaaga atacataaaa aagaatatga gattgggaaa
    20401 tgggctgtag attatataaa taaagaattt gatgtgaaat ttccagtaga tgaagctggt
    20461 tttatagcaa tgcatatagt aaattcaaac tataagggaa gttcaaagga atcactgtta
    20521 attactaaaa ttgtaaaaga catactaaat attataagat attattatag agttgaattt
    20581 aaagaagatg atataaatta tgataggctt ctgactcatt taaaattctt tgcaaaacga
    20641 ttagtaaaaa aagaaaaaat caatgataca aataatgaaa ttatagatat aattaaagta
    20701 aagtatgaaa aggattatga ttgtgcatat aaaataaaaa ctcatgtaga a
//
LOCUS       DAEMHW010000054        20603 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEMHW010000054 DAEMHW010000000
VERSION     DAEMHW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.054"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            445..1515
                     /locus_tag="KPK67_001702"
     CDS             445..1515
                     /locus_tag="KPK67_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2458688.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1499..2368
                     /locus_tag="KPK67_001703"
     CDS             1499..2368
                     /locus_tag="KPK67_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG2458689.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2412..3272
                     /locus_tag="KPK67_001704"
     CDS             2412..3272
                     /locus_tag="KPK67_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG2458690.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3303..4607
                     /locus_tag="KPK67_001705"
     CDS             3303..4607
                     /locus_tag="KPK67_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2458691.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSKHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4670..5932
                     /locus_tag="KPK67_001706"
     CDS             4670..5932
                     /locus_tag="KPK67_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBG2458692.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6119..8089
                     /gene="uvrB"
                     /locus_tag="KPK67_001707"
     CDS             6119..8089
                     /gene="uvrB"
                     /locus_tag="KPK67_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBG2458693.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8108..10933
                     /gene="uvrA"
                     /locus_tag="KPK67_001708"
     CDS             8108..10933
                     /gene="uvrA"
                     /locus_tag="KPK67_001708"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG2458694.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11192..13009
                     /gene="uvrC"
                     /locus_tag="KPK67_001709"
     CDS             11192..13009
                     /gene="uvrC"
                     /locus_tag="KPK67_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBG2458695.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13009..13938
                     /locus_tag="KPK67_001710"
     CDS             13009..13938
                     /locus_tag="KPK67_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBG2458696.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEVWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14152..15948
                     /locus_tag="KPK67_001711"
     CDS             14152..15948
                     /locus_tag="KPK67_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBG2458697.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVITGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWSLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16088..17869)
                     /locus_tag="KPK67_001712"
     CDS             complement(16088..17869)
                     /locus_tag="KPK67_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG2458698.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAVLKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17892..19778)
                     /locus_tag="KPK67_001713"
     CDS             complement(17892..19778)
                     /locus_tag="KPK67_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBG2458699.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19795..20292)
                     /locus_tag="KPK67_001714"
     CDS             complement(19795..20292)
                     /locus_tag="KPK67_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBG2458700.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
BASE COUNT         7990 a         2725 c         3511 g         6377 t
ORIGIN      
        1 gggtaaattt ataaaaatta aaaaagtttt attttttatg tttaaacctt gtaaatttgg
       61 gacataatat aattgtaaaa cttaatatag caaaaataaa ttactcaatc tcccccactt
      121 aaagacctct atcccccata gaggtctttt ttactttcaa atcaaattat ataaaaaata
      181 aatagtttta tatgactctt cataaattaa aattacattg tgatagcaaa ccatgaatat
      241 gaaaaatata gaaaattata gctaacataa gctgtatatc atcttaactt cagtatattt
      301 aacaaaaaat gtttataatt taacctttat tttaataagt ttaccatata ttcactttgt
      361 cttaatatat cctttacaaa ttaagagtat tattaatcta taagtaaatc gtagaattaa
      421 attaaatatt ttgggaggat aaaaatggca gatataagac tagaaaatgt aacaaaatct
      481 tataataaaa aaaatacagt tatagaaaat ttaaatctta ctatagaaga tggttctttt
      541 acagtattgg taggcccaag tggatgtggt aaatctacta cgttaagaat gattgcaggt
      601 cttgaagata taacttcagg taggttgaag attgaagaaa atgatgttac aaaaacggat
      661 gctagtaaaa gggatatagc tatggtattt caaaattatg cattatatcc acatatgact
      721 gttagagaaa acatagaatt tggtcttaaa aacaaaaaag tagaaaaaga aaaaagagaa
      781 aaacttataa cagaagttat tgatgtagta ggattgagag aatatttaaa tgtgaaacca
      841 ggcaacttat ctggaggtca aagacaaaga gttgcacttg ctagagctat ggttaaaaat
      901 ccaaaggtat ttttaatgga tgaaccattg tcaaatttag atgctaaatt aagaaatcaa
      961 atgagaacag aattaataca attacatcaa aaactaaagt ctacatttgt ttatgtaact
     1021 catgatcagg ttgaggcaat gtcgatggca gataagatag ttataatgaa caaaggaaag
     1081 ataatgcaaa taggttctcc aaagaagata tatcaaaatc ctaaaaattt atttgtagct
     1141 caatttatag ggagtccttg tatgaacata ctagatataa aaaatgatat taaatttgga
     1201 ttcagaccag aaaaagcaac actagagagt ataaacatag atggaaaaaa tgatgatgta
     1261 tttagtctat ttggaaaagt tgtaactaaa gaagtgttgg gaaatgaaac tatatattgt
     1321 ttatctgttc ttaatagaga gataagagtc aaaacggaaa atgatatatt tgatattgga
     1381 agaactttaa gaataagtgt attagaaaaa gatttatatt attttgataa aaatggagaa
     1441 agaatagaag atgtaaaagt atgtaaaagt atgtattcta aaattaaagg agatatttat
     1501 gaaaaagcaa tttaataaag catatttata tataacacct gctattatag gaactttatt
     1561 gtttatagtc tatccaataa ttaagacgat atctctaagc ttcaaatctg gtagtcttct
     1621 tagttcaacc tttgaaaatg taggcttaaa caattataca gaacttttaa agaatccaga
     1681 gtttatacaa actataacga acactgcaat ttatactttt tttatggtga ctatatctat
     1741 atcactagcc atagtacttg ctgtttggct aaataaaaat tctataatac acaatttaac
     1801 gcaaagtata atctttacac cacatgtaat ttcactagta tctgtagctg tattgtggct
     1861 ttggattatg gagccacaat atggcttttt aaactggata ctatcgacat tacatttacc
     1921 aacatcaaag tggctttcaa gtgaaagttc agctttaatg tcactaattc taataggaat
     1981 atggaagact cttggataca atatcttaat agtgttatca ggtcttaaat ccataccaaa
     2041 ctatatatat gaagcatcta aattagataa tgcaaacaaa ataacgaatt tctttaaagt
     2101 gacattgccg ctattatcac caacaatatt ttttcttatg ataacaacaa caactgcatc
     2161 ttttcaagta tttgatgaga taagtataat gacacaaggt gggccagtgg gttcaactaa
     2221 catgttatct tattatatat atgagtctgg atttatatac tatgacatag ggcaagcgag
     2281 tgcagcgagt atgtttttgt tagctatagt tatgattgct acatatgcta actttagact
     2341 cttatcaaag aaagttcatt atcaataaag aaaaagtaga tattataagg tttatccaaa
     2401 aggaggagac aatggatatt atacaaccta tcaaaaaaga caaagttgat ataaaaaaaa
     2461 tagcatcaaa atcaatagaa atattaattc tattactttt atctgcaata tttatattcc
     2521 cttttatatg gatgctatct acatccttta aaaatccaca agaaatgatg caattaccgc
     2581 ccactattat accaaatgat atagtgttag aaaattatag tacagcatgg cattcaggtc
     2641 cattttttag atatttgcta aacagtgtat ttgtaacagg aagtgtttta attgtacaat
     2701 ttttagttat ggttccagca gcatatgcat tttctaagat taaatttaaa ggtgaaaaaa
     2761 tgctatttgg gcttttgctt gtaggactta tgattccacc acaagtaact tttctaccag
     2821 tctatatgat gatgagtaag tttggattta taaatactta tgttccactt ataattccat
     2881 ttatgacatc ttcatttggg atatttctgc ttagacagaa ttttatgcag atttcagatg
     2941 aaattattga agcagctaaa ctagataatg cttctgactt aaaaataatg tttagaataa
     3001 tggttcctat ggcaaaacca gcagtaataa catttatatt atttaatttt atataccatt
     3061 ggaataatta tttctggccc ttagtaatga caaatacaga tgcaataaga actttaccaa
     3121 taggagttgc gctccttaaa tcttcagaag gaattactgc atggaatgta ataatggcag
     3181 gaaatataat tctaatttta ccaataattc ttgtttatat atttgcgaat aaaaaagtaa
     3241 aagaagcttt tatgtattca ggaataaaat aaaaaattaa agactaaatt tggaggatga
     3301 aaatgaagct taaaagaaaa ttagcaacag gattagcaat aacattatta ttaggttctt
     3361 tagttgggtg ttcaaattct aatgatgcaa atgcatctaa aggagaaaag actgatagca
     3421 atattacgaa gatagatttc tgggctcctt taggtggaac taatggagaa actgctcaaa
     3481 aaatggttga ccaatttaat agtaaacata aggatgtaca agtaaatatg ttaaaacaga
     3541 aagattacta tgaaaatgca acaaaactac aggcagcctt aacatctaaa gaccaaccag
     3601 atgtaacatt attagaaata actcaaacag gtacattcgc atcagcaggt gctttagtag
     3661 atatgagtaa atattttgat aaaacatatc aagaaagatt cttttctggt cttttaacaa
     3721 attcatatta tgaggataag tttgttggta tgccatttaa tagaagtaca ccaatattat
     3781 acataaataa agatatggca actaaagcag gtcttgaccc ttcaggtcca aaatcatggg
     3841 aagagcttaa ggattatgct agtaaaatga caaataaatc agaagatact tatggttttg
     3901 aaacaccaat agatatttgg ttctatgaag ctatggtaat gcaaagtgga ggagaaataa
     3961 ctaatggtaa aaaagtagca tttaataatg aggcaggaca agcaccagtt aagttctggc
     4021 aagatatgat gaaagctgga ataatgaaaa tgcctcctgg agaagattat aatgcctggg
     4081 acgtagcaaa acaagatttt gtaaatggta aagtaggtat gatatttaca tcaacagcag
     4141 atttagcagg acttatgcaa caaacagaag gtaagtttga aatatcaaca gcattcttac
     4201 caaaaaatca aaaatacgca actcctacag gtggagctaa tctagttatg cttgaaggtg
     4261 gaacagataa agaaaaagaa gcaagtgcag aatttataga atggatgaca caaacagata
     4321 aaatcgttca gtttagttca tcaacaggat acttaccaac aactgaagat gcaacaaact
     4381 cagaaaagtt acaaacatta tataaagaaa aacctcaata taaggttgca actgaccaat
     4441 tacaatatgc tgttgcactt ccaatgttaa atggatacaa ggaagcaact gataagctaa
     4501 tggatgagat taaaaagggg cttactgatt taaatgcaaa tccaaaagat gtagtaagca
     4561 aaggaacttc tgcaatgcaa gcagtaatag ataaaacaaa taaataatta tatagacttg
     4621 taagttgatt aaaagtattc aataatatat aaatgaagag gtaatgtaaa tgacaaaggt
     4681 tatagtatta tcagtagatt ctttatttga aaaagacttg gaatttgtaa aaaatctacc
     4741 aaactttaaa tctatactag aaaattgctc catagtaaaa aatataaatt gtgtttatcc
     4801 cactctgaca tatccgtgtc atactagtat ggttacagga gtatatccta aaaaacatgg
     4861 tatatatcat aatgaaaaat ttgacccaaa taaagaaaat aaagactggt actggtattc
     4921 aaaggacata aaagcaaaaa ctattataga tgtagcaaaa gaaaataatc taactacctc
     4981 tagtgtatta tggcctgtaa tgggagcaaa tccaaatata gattataata ttgcagaaat
     5041 atgggctcct agtagagaag atgacccaag agaaactttt gaacgaagtt ccagtaaggt
     5101 tataatggac ggtatttaca atagacattg tcattatatc gattggaaat tagaacccaa
     5161 catggattta tttggagtaa actgttcagt agatataata aaggaatata aaccagattt
     5221 aatgctaata catttagcta cattggacca tacacgacat aattatggat tatttaatga
     5281 cgaagtagat aaagcattaa aaatgaatga taaatggcta ggagatatta ttcaggctac
     5341 aaaggatgca ggtacataca aagatacaaa ttttataata cttggggacc atggtcatct
     5401 tagagttgat aaagttataa atccaaatgt cttgcttaaa tcaaatggat ttataaaagt
     5461 tgaaaatgga gaagttaagg attttgatgc ctatgtaaat tcttctggaa tatcagctca
     5521 aataatagta aataacttag ataggttaac agaattaaga gaactgcttg aagaatttaa
     5581 agaagaatta ttcatagaga atatttttac caaagaggaa gcatctaacc ttggtcttga
     5641 aggtgatttt gaaatggtaa ttgaaggtct tgaaggaatt tcatttggaa atgatttcga
     5701 aggaagtatt ataaaagatt cagatataaa agattacaag tttgctgttg cgacacatgg
     5761 tcatttacct actaaaggta atagacctcc atttatagct tttggaccaa atattaaagg
     5821 tggtgtggtt atagaagaag gagacctgag agcacatgca gcaactatat taaaaatgtt
     5881 taatttaaaa ttagatggag ttgaaaaaga agcatttgat tttattatgt aagttgaata
     5941 aagtatttat tttccatgaa ttatagactt ctattgcaaa gatagaagtc tttttttatg
     6001 ttttagattt aagtaaaatt taaatgggta aaatatttaa aataacttaa acaattttat
     6061 aaaggtatga tataataata catgtcagga actaatgttc gaaaaaagga gtgatactat
     6121 ggattttaaa ataaaatcag acttcaaacc tacaggtgac caaccagaag ctattaaatc
     6181 aatagtggat tctataaata gaaatgagaa attttcgaca ttattaggtg taactggttc
     6241 aggaaaaaca ttcacaatgg caaatatcat tcaacaagtt aagaaaccta ccctaataat
     6301 ggctcataat aaaactctag cagcacaact ttacagtgag tttaaagaat ttttcccaga
     6361 caatgcagtt gaatactttg taagttacta tgattattat caaccagaag catatgtagc
     6421 tcacagtgac acttatatag aaaaagatgc aagcataaat gatgaaatag ataaacttcg
     6481 acattctgct acagcatcta ttttagagag aagagatact ataataatat catctgtatc
     6541 ttgtatatat ggacttggtg acccaaaaga ctataaagaa cttatgttat ctataagacc
     6601 aggtatgcaa agagacagag atgatgtaat aaaaaggtta atagagatac aatacgaaag
     6661 aaatgacata aactttacac gaggtacctt tagagttaga ggagatatac tagaaatatt
     6721 cccagctagt aatgatgaaa aagcaataag aatagaattt tttggtgatg aggtagatag
     6781 aataacagaa attgattatg taacagggaa gattgttggt actagaaatc atgttgttat
     6841 attcccagca tctcactatg taacaacgcc agaaagaatt gaaaaggcaa ttgtagaaat
     6901 agaaaacgag cttcaagaac aaattaaatt tttcaaggaa aatgataggc tattagaagc
     6961 acaaagaatt gaacaaagaa ctaagtatga tatagaaatg ttaaaggaaa taggattttg
     7021 tcaggggata gagaactatt ctagacatat tacaggaagg tcagaaggag aaagaccata
     7081 tactttaatg gatttcttcc cagatgatta tttgataatt gtagatgagg cacatgtcac
     7141 tataccacaa gtaagaggta tgtatgcagg agacaggtct agaaaaacat cacttataga
     7201 gaatggtttt agattacctt ctgcacttga caatagacca ttaaattttc aagaatttga
     7261 aggaaatata aatcaaatgt tgtttgtaag tgctacacca ggaccttatg aaattcaaca
     7321 ctcagagaca atagcagaac agataataag accaactggc cttttagacc caattgtaga
     7381 agtaagacct ataaataatc aaatagatga tttggtagga gaaataacta aaactataga
     7441 aaaaaatgaa agagtgctta taaccacttt aactaaaaaa atgagtgagg acttgactaa
     7501 ctatcttaaa gaaataggga taaaagttaa atatttacac tcagatatag ttacattaga
     7561 aagaacagaa ataataagag atttaagatt agggaagttt gatgtccttg taggaataaa
     7621 cttacttaga gaaggattgg atataccaga agtttcactt atagcaattt tagatgcaga
     7681 caaagaaggt ttcttacgtt ccgaaacagc tttaatacag actattggac gtgcagctag
     7741 aaatgaaaat ggaagagtaa ttatgtatgc agacaggata actgactcaa tgcaaaatgc
     7801 tattgatgaa acaaagagaa gaagagatat acaaaattta tataatgaag agcacaatat
     7861 cattcctaaa actatacaga aaaatataag agatagtata gaagctacta aggttgcaga
     7921 ggaagaagtt gtatatggaa tatctgatac agatgataaa gacgaaataa gagctaatat
     7981 agataaatta aaatcagaga tgatggaagc tgctcaaaac ttacaatttg aaagagcagc
     8041 agaacttaga gataaagtga aacaattaga agaaaaattg gaaaaataga aaggtaaaaa
     8101 caacattatg gaagataaaa ttataataag aggagcaaaa gaacataatt taaaaaatat
     8161 agatttagaa ttaccaagag ataaatttat tgtttttaca ggtttgtcag ggtctgggaa
     8221 gtcttctttg gcatttgata caatatatgc agaagggcag agaagatatg tagaaagtct
     8281 atctgcttat gcaagacaat ttttaggtca gatggaaaag ccaaatgtag aatatataga
     8341 gggtttatca ccagctatct caattgacca gaagactact tcaaaaaacc cacgttctac
     8401 ggttggaaca gtaacagaaa tatatgatta tttgagacta ttatttgcaa gagtaggaga
     8461 tgtgtattgc cctacatgtg gtgaaaaaat aagtcagatg actattcaag aaatagttga
     8521 taaaatgcta gattttccag atagaacgaa attacaaatt ctttcaccaa ttgttagagg
     8581 acaaaaaggt acacataaaa aagtctttga taatatcaaa aaagaaggat ttgtaagagt
     8641 tagagttaat ggtgaaaact atgaagttag tgatgatata atcttaaata aaaatcaaaa
     8701 acataatata gaagttgtag tggacagaat agtaattaaa gatggaatag aatcaagact
     8761 agcagattca attgaaactg cagttaagct atctgatggt cttgtaatag cccaaataat
     8821 agatggagaa gaaattcttt tttcaactaa atttgcatgc ccagaacatg gaattggcat
     8881 agaagaatta tctccaagaa tgttttcatt taatgctccc tttggtgctt gtgatgtttg
     8941 taatggtctt ggggaaagta aagaagttga cccagaatta gtagtaccaa acaaagattt
     9001 atcaataaaa caaggtgcag tggcagcatg gggttcaaca ggtgttaatg atgacactta
     9061 ttacagtaaa atgataaaaa gtttagcaga gcattttaat gtatctttga caacaccgtt
     9121 taaagattta ccagatgatt ttgtggagga attactttat gggaaagata atataattgt
     9181 agagtttact tatgagtcaa aatttggagg aactagaaat tataaatcat actttgaagg
     9241 tgtaatagta aacttagaga gaagatatag agaaactaat tcagattaca tgagagataa
     9301 gattgaagaa tacatggcag agagaccatg tccaaagtgt aaagggatga gacttaaaaa
     9361 ggaagtttta tctgttttag ttgatggaaa aaatataatg gaagttacaa acttatctgt
     9421 aaatgaattg attgacttta tggaaaatat aaatcttaca gaaaaacaga gatttatagc
     9481 tcatgagatt ataaaagaga taaaaggaag agctatgttc ttaagagatg taggtcttga
     9541 ttatttaaat ttatcaagaa aagctggaac tttatcagga ggagaagcgc aacgtataag
     9601 attagctact caaataggtt ctgcattggt tggagtttta tatgtacttg atgagccaag
     9661 tatagggctt caccaaagag ataatgatag gcttataact actcttagac atctaacaga
     9721 tttaggaaat accttaatcg ttgtagagca tgatgaagac acaatgagag aagctgatta
     9781 tgttatagac attggtccag gtgcaggtat acatggtggt gaaatagttg cacagggtac
     9841 tttagatgaa ataattgaaa atgagaattc aataacgggt caatatttaa gtggtaaaaa
     9901 acaaatattg cttcctgaga ctactagaga aggaaatgga agttttatag aaatagttaa
     9961 agcatcagaa aataatctga aaaatattga tgtaaagttc ccattaggta agtttacatg
    10021 cataacaggt gtttcaggtt ctggaaaaag tacattaata aacgatatac tttacaaagg
    10081 tgttgcaagt aaagtgaata gacttaagca aagagctggt aagcataagg agataaaagg
    10141 aattgaaagt atagataaag ttataaatat agaccaaagt cctattggaa gaacacctcg
    10201 ttcaaatcct gctacttata caggtgtatt tgattttata agagatattt ttgcatctac
    10261 taatgaagct aaagcaagag gttataaaaa aggtagattt agttttaatg taaaaggtgg
    10321 tagatgtgaa gcttgtaagg gtgatggaat aataaaaata gaaatgcatt tcttaccaga
    10381 tgtatatgtt ccttgtgaaa tttgtaaagg tgaaagatac aatagagaaa cattgcaagt
    10441 aaaatacaaa gataaaacta tatctgatat attagatatg aatgtggaag aagctgttga
    10501 gtttttcgaa aatataccaa atataaaaag aaaattagaa actttaatgg atgtaggact
    10561 ttcatatatt aaattaggac agccttcaac tcaattatca ggaggagaag cacaacgtat
    10621 aaaattagct gctgaactta gtaaaagacc aacaggaaag actttgtata tattagatga
    10681 acctactact ggacttcata tggcagatgt agacaagcta atccaagtat tacaaagact
    10741 agcagataca ggaaatacaa ttatagtcat agaacataat ttagatgtta taaagacttg
    10801 tgattatgta atagacttag gaccagaagg cggagataag ggtggaacta tagttgcaac
    10861 tggaacacca aaagaagtat ctaaagttga tggttcttat acaggtcaat tcttaaagaa
    10921 atatttttct taagttatat aaattgtgaa aaattttaga gcagatatac gataaactgt
    10981 tttgttataa gtaaattagt caaatataag ttgtaaatag taatacaaag taaagtccat
    11041 attactcttc atggttaaaa ttgttttcaa atcaattata gaagggaata tgggctttaa
    11101 cttgtctaaa gcactggaaa ttgtataact ttttaaaatt catattttag ggtataattt
    11161 tagtataaaa tttaatgttt tggagggcga gatgtttgat atacaagagc atttaaaaaa
    11221 attaccttct gagcctggag tatatttaat gaaagacaaa tacgaccata taatatatgt
    11281 gggaaaagcc atatctctaa aaaatagggt aaggcagtat tttcaatctt caaaaaacca
    11341 tacatctaaa gtaaaatcta tggttaagaa tatatataag tttgagtata ttataacgga
    11401 ttcagagtta gaggcattaa tacttgaatg taatttaata aaaagataca gaccaaagta
    11461 taatgtagtg cttagagatg ataaaactta tccatatata aaagtaacta cgaatgaaga
    11521 ttatccaaga atcttaaaag ttagaagagt tcttaaagat aaggcaaagt attttgggcc
    11581 ctataccaat ataactgctg tgaatgatac cttggagctt ataagtagta cgtatccaat
    11641 aagaagctgt aaaatagata tagataaagc aataaagaat aagacaagac cttgtttaaa
    11701 tctacacata aataaatgtt taggaccttg tacaggaaat gtttctaaag aagagtatgg
    11761 aaaaatgata gaagagatta taatgtgttt atctggtaaa gaagaaaaat tgatggaact
    11821 tttaaaggaa aaaatgaatg agagttctat gaactttaga tttgaagagg ctgctgtata
    11881 tagggataag ataaagagcc ttgaagagat gatacagaaa caaaaaatag atgctactgt
    11941 aagtgactta aaccaagatg tggttgcaat ggcaagagct cataatgaag cttgtgtcca
    12001 ggtatttttt ataagaaatg gtaagattgt aggtagggaa cattttatat tagaaggtgt
    12061 aatggatagt ccaagagctt ctatattaag ttcatttgta aaacaatttt ataatgaaca
    12121 agagtatata cctaaagaat taattataga agatgagata gaagatagtt ctatattaga
    12181 agaatggctg tcatctaaaa aggggcaaaa agtaacaata agagttcctc aaaagggaga
    12241 gaagaaaagc ttagttgaaa tggttaggaa aaatgctgta gaatatcttg aaaaattttc
    12301 tgatatgaat aaaagaaaat atgaaaaaag tattggagca ttggaagaat taaagcaaat
    12361 attaaatcta gaaaagttgc caataagaat agaggcttat gatatatcaa atatacaagg
    12421 tgtagattcg attggttcta tggtagtgta tacgaatgct aaaaaagata agaaggaata
    12481 taggagatat aaaataaaga cagttattgg acctaatgat tatgattcta tggctgaaat
    12541 tgtagataga agattaaaac atggaaattt acctgacttg atattgttgg atggtggaaa
    12601 aggacaagtt agtgcagtaa aaaaagtact agaactcaat gatgtagata tacctctttg
    12661 gggaatgtac aaagatgata agcatagaac taaaggattg atatgcaaag aaaaagaaat
    12721 agaactagat aaaacaacta atttatatag atttattgct agtatacaag aagaagttca
    12781 taattatgca ataacatatc atagaagtct aagaaataaa gctttgacaa aatctatctt
    12841 agatgatata caaggaatag gtgaaaaaag aaagaaatca ttattaaatc actttaaaga
    12901 tgtagatgct ataaaaaaag ctactatgga agaactttta gaagtggatg gaatgaataa
    12961 aagcattgca gataatgttt ataatttttt tagaaaggaa gaaaattaat ggaaactagc
    13021 aataaagtat caataagaga gataataaaa gagttgaagt taaaagtagt atacatgcct
    13081 gatgatgtag attattatgt ctactctcaa gatataaata gaccaggatt acagtttgca
    13141 ggatattttg attattttgc atatgaaaga atacaaatag ttggaaagac agaatataat
    13201 ttttttggat taatggatag ccatattcga gaagaagtac tagatagatt tttctcatat
    13261 gaaataccag cgttaataat ttctagagac ttggaaatca agccagatgt aattgaaaag
    13321 gctaaaaaat ataaaagaat acttttgagc acaaatagaa ataccactag acttataaat
    13381 agactgtcaa actatttgga taataagtta gcaccacaca caacaattca cggggtgcta
    13441 gtagatattt atggtatagg tgtactgata aaaggtgaaa gtagtatagg aaagtcggaa
    13501 acagcattag aactgattca aaggggtaat agattagttg cagatgatgc tgtagaaata
    13561 agaaaattag atgaaagtat gcttgtgggt cagtcacctg aactaataag acattttctt
    13621 gagataagag gaataggaat aatagatgtt agaagtttat atggagtagg ggcaatcaaa
    13681 aattctaaaa tgatagattt agtagttcat ttagaagttt gggatgaaac taagtattat
    13741 gatagactgg gactggataa agaatatgaa gaaatacttg gtgttaatgt agagaaatta
    13801 gttgtacctg ttaaacctgg aagaaatact gctatgatat tagaagtagc agcaatgaat
    13861 tttagacaaa gaggaatggg atacgatgca gctcaagaat ttactaaaaa attatcaaaa
    13921 ttaatcgata ctaagtagta ctgaataata gtggactaca catcatttaa ttaaatattg
    13981 attaggatta tgtcctacac cttataaatt aaaatcaagt tgtatatata tgatgtttat
    14041 taataagata tattatataa ggataattta aagtacagtg ttagattata ataatactag
    14101 attaatttta tatttgagtt tcattatata acaagaaagt tggaattttt tatgggaata
    14161 gaatatttta gtaaactttt agaaagtagt actgaaaaag aattgctctt aagtaaaaag
    14221 tcaaataaca taagtacagc aagattaata tcttttttga tagtgataac agggcttgct
    14281 attggatttt acaataaaaa tatagtaggt atttttgttg gagttttatc aataataata
    14341 tttatagcac tcttagttat tcatagtaaa gtaaaagaag aagaaacata ttttaaaagt
    14401 aagagcgaag tattaaataa gtatattaag agatttggag atggttggaa ggaatttaaa
    14461 atagatggaa aagaatactt aaaagatgaa aactcgcaag caaaagattt agacttattt
    14521 ggaagagcat cattgtatca atatatctgt gtagctaata catcttatgg gaaaaatttt
    14581 ttagccaaat atttgtggaa tgaaaatcca gatgagaatg taatcttaga aagacagagg
    14641 gctataaaag aacttttatc aaaagatgat ttttctattc atattcaaac actcagtaat
    14701 ataattggta aagagcaaaa aaacaattca gatagtagta tagaatcgtt tatagagtat
    14761 ggagaaaata agaatgtgta tataccaaaa tggatgcata tttttacttg gagtttacca
    14821 actgcaacaa tcttatcttt tatattttgt atgttaggat ttttacctgt tttacctgtt
    14881 tttttacttt ttataatcca gttagggttt agtggttttg gatatccaag acttatgcaa
    14941 actcttgctc cattattttc atttagtaga agtatacaag tatatgaaaa aatgtttgag
    15001 gttttagaaa aagagacgtt tgaaagtgca tatttgaaag aattacaaga taaactttct
    15061 aagggaagtg gagtttcaag aggtgttaaa caattaaatt caataggaaa tgctgtaaac
    15121 ctaaggtata atcagatact atatataatt gcctgtggtg ttttgatgtg gaactatcat
    15181 tgtgcagaag ctttggagcg atggaaaggt atatatggaa atcaaattag agattggttt
    15241 gaatctattg gagaatttga agctctaata agtttaacag ttgtttctca tgtaaaggaa
    15301 aatacatgtt ttccagtaat aaaatatgaa gatactccaa gattgaaagt tgaagaggta
    15361 taccacccat taatagcaga aaaaagtgtc atagcaaatt cgatacaatt aaattctcag
    15421 acttgtatta tcacaggttc aaatatgtct ggaaagacaa catttttaag aagtatcggt
    15481 gtaaacttag tgctggcata tgcaggtgct ccagtatgcg ctaagagttt tgatgcgacc
    15541 tgtatggcta tatttacttc tatgagaata caagatgatg taagtcaagg aatatcaact
    15601 ttctatgctg agattcttag aataaaatca atgattcaat atagtttaaa agaactgcca
    15661 atgttggttc tagtagatga aatttttaag ggaactaact ctgctgatag aattattgga
    15721 gcaagtgagg cagtaaagaa attatcaaaa ccctgggtta taagtatggt gacaacacat
    15781 gactttgagc tatgtgacct ttcaggtagc ggagatgttg aagttgtaaa ctatcatttc
    15841 tcagagtact atgtagacga taaaattcat tttgactata agataaaaga tggaagatgt
    15901 aaaactacaa atgcaaaaca acttatgaga atggcaggta ttctttaatt aatagatagg
    15961 ttaattaata aataagtcta tttagaaata taatttaatt tgtgaaatta ataaggattg
    16021 tcttaaatta cgtaaaagta ttttaagaca atcctttttt aagaataaat tttatacttt
    16081 gttatgacta cttagcttct tcacaagcac attcttgtgg acactctcca tagacacaac
    16141 tcttatcaaa gtaatgagta tgaaggtatt tatgagctac atcactattt ggttctccaa
    16201 agaagtcttt ataaatagct tgtaaatcag gattctcatg agatttacga atagccttat
    16261 ttctatctat ttcatataac gcatttgcac gtctatcaac tatttcagta ttaccatggt
    16321 gataaggttg accagcacct ccaacacatc caccaggaca tgccataact tctatgatat
    16381 ggtatttaca ttctccactt ctgaccttat ccataatctt tctagcgttt ttaagtgtat
    16441 ttacaataca cacattaact gtagttccat caacatctat acttgcttcc ctaattcctt
    16501 taagacctct aacattagtg aagtttacat tatctaaagt cttattagtt atcttctcat
    16561 atgaagtacg tagggcagct tctaaaactc caccacttgc tccaaatata gatgcagcac
    16621 ctgtagattt tcctaaagga ttatcaaaat cttggtcttg aagattaggt aaatcgatag
    16681 cagcttcttt tatcatctta gctaactctc tagtagttat agaaaggtca acatctagta
    16741 ttccagattc acttaattct tctcttgaag cttcatattt tttagctaca caaggcatta
    16801 cagatacaac ataaagttct tcaggattga ttcctaatat tttaggtgca tagtagtttt
    16861 tagctataga accaaacata ccttgaggag acttacagct agatgctaag tttaacttgt
    16921 ctggataatt gtgctctaag aagtttaccc atgcaggaca acaacttgtt aatataggaa
    16981 gattttcacc tttttggatt ctttctataa attcagtagc ttcttccatt atagtaaaat
    17041 ctgcaccaaa gtttgtatca aatacatgtt caaatccaag tgcttttaaa acagctacca
    17101 tttttcctgt agagattgac cctggttcta atccaaactc ttcaccaata gcaactctta
    17161 cggcaggagc aacttgcact actatagtct tttctttttt atttaaaaca tcccaaagtt
    17221 taggtacatt atctacttct gttaatgctc cagttggaca gacagatata cattgaccac
    17281 agaatgtaca ctctgtttca accatatcag agttaaagaa tgtactaact aaagtattaa
    17341 atccacgatt aactccagat aatgctccta ctgtttgtat gtcattacat acagtctcac
    17401 atcttctaca taatatacac ttagaatggt ctttaactaa agatttagta gaagtatctt
    17461 tacctgcaaa agatttggaa ccttgatatc ttatttttct tactcctaaa tcagcagcta
    17521 tagtctgtaa ttcacaatct ccattttttt cacagacaaa acaatcttgt ggatggtctg
    17581 ataacaataa ttcaacaata gttctacgag catttaatgc tttagcacta ttagtctgaa
    17641 ctttcatacc ttcctttata actgttccac aagctggaac taatcctctc tctgtctcaa
    17701 ccatacaaac acgacaagaa gcacaagtgt caagcttatc tatttcattc atatgtaaat
    17761 gacaaaggtt gtgtattttt atatttatta atttcgcagc atctaagata gaagttccac
    17821 taggtgctga aacgtgtttg ccattaatag ttaaattaac taaactcatt gttattcctc
    17881 ctccaaataa gttatttctt gattatagcg ttaaatgtac aattatctat acaagcacca
    17941 cattttagac acttagaagt atctattgta tgttgttcct taacagaacc tgttatacaa
    18001 ccagcaggac acactctaga acattttgta caacccttac agtcatctgt tataaagtaa
    18061 gaaagtaagt tctgacattt accagcagga catttcttat ctacaacatg agctatatac
    18121 tcatctttga aatattgaag agtacttaaa acagggtttg gagcagcttt accaagacca
    18181 cataatgaag cagtttgtat agtttcagca aggttttcta ggtcatctaa atcttgtaga
    18241 gtaccattac cttctgtaat tttagttaat atttctaata atcttgtagt acctatacga
    18301 caaggtgtac atttaccaca agattcttca acagtaaact caaggaagaa cttggctata
    18361 tctaccatac agtcagattc atcaagaaca agcataccac cagagcccat cattgaaccg
    18421 atagaactta atgaaccaaa gtctatagga gtgtctaaat gttcagttgg tatacatcca
    18481 ccagatggtc cacctgtttg aacagcctta aatttcttac cttcagggat tcccccacca
    18541 atttcaaata caatatctct taaagtagta cccattggaa cttcaacaag tcctacattt
    18601 tctacttttc caccaagagc aaagacttta gtaccactag aatcttcagt acctaagttg
    18661 gcaaaccaat ctccaccctt aagtataatt gcaggtatgt tagcaaaagt ctcaacgtta
    18721 tttaagcaag taggagattt ccatagacca ctcttagaag aactataagt tttcattcta
    18781 ggttcaccac gtctaccttc aattgaatgc attagcgctg taccttcacc acaaacgaaa
    18841 gcacctgcac catattttaa ttctaattta aagttaaatc cagtgcctaa gatattttcg
    18901 ccaagtaaac cgtatttttc agcttgagct atggctactt ttaatctttc tatagatttt
    18961 ggatattctg ctcttatgta tatgtatcct gtatcagaac ctatagcgta tccacatatt
    19021 gccatagctt ctagtacact atgagggtca ccttcaagta cagatctatc cataaatgca
    19081 cctgggtcac cttcatcagc attacataca acaaactttt tatctgttgg accagctgga
    19141 ttttttgaag cagcttccca tttagagcca gtagggaaac ctgcaccacc tctaccacga
    19201 aggccagaag tttttacctc tgcaataact tcttgaggag taagagaagt taaacatttg
    19261 cctagagcta aataaccatc attggcaata tattcttcta tactatctgg gtctataaga
    19321 ccacaatttt ttaaagctat acgtaactgt tttttgtaga aagacatttc ttcttgttct
    19381 tcaacttttt tacttaaaga ttcttcctca tataataatt cctcgacaac tgtatttcta
    19441 attaagtggc tttgaactat cttttcagca tcagatggct caacttttac gtagaaaacg
    19501 ttatcaggat aaacttttac aataggacct tgagcacaga aaccaaaaca acctgtcaaa
    19561 cgaacactaa ctttatcttg gattcctgat ttctttattt cattttctaa ttcactaact
    19621 atttctagac tgtttgaaga tgtacatcca gtatcacaac aaacaagtaa ttctcttctt
    19681 aatacatttt ccttagaata attttttctt aagctaagat taggtttcag ctcatcagct
    19741 aaaactttta attcatcaaa tgaatttact ttacgcatta tttattcacc cctcttaaca
    19801 attcaattct aattctctat aagtatctaa tatttcgctt acttggtcag gtttcacttt
    19861 accataaact ttaccattta cagtaacaac tggagctaaa ccacatgccc ccaaacatct
    19921 taaaccttct aaagaaaatt taaaatcact agtagtttct ccaactttta tgccaagctg
    19981 tttttcaaaa gcgcttaaga ttttgtcagc accttttaca aaacatacag ttcctagaca
    20041 gacactgatt ttatacttac ctacaggttc tgtagtaaaa taagaataga atgtaacaac
    20101 cccatatacc tttgcaggag ctactccaag ttttctagca acatgaagtt gaacttcttt
    20161 tggaagatat ccaaatatac cttgggcttc atgtagaact tgtattaacg caccttcctt
    20221 tgttgcaaga gagtcaataa atatatctag ttcgtcaaat aattgcttgt tgtgtgaaat
    20281 aaaatcgcac ataacaaatc ccccttttct ttttaaagtt attatttttc ataaaataat
    20341 taaccatgag ttgaccccaa cattatgtgg gtgaacaaaa ccaaaacaat aaagaaagaa
    20401 taatatacaa caatttcttt atttataaaa acaatgcaaa aaagttacct attaaaaata
    20461 aaaactatac tagtctatta taatataaaa taattcagaa tagttaaaaa aaataaaaaa
    20521 aatgagctaa attcaataaa aaaaaaataa tacatataaa aaactttaaa aaataaacca
    20581 aaatgtaaaa tttttataaa ata
//
LOCUS       DAEMHW010000055        20532 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEMHW010000055 DAEMHW010000000
VERSION     DAEMHW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.055"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            78..1001
                     /locus_tag="KPK67_001715"
     CDS             78..1001
                     /locus_tag="KPK67_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2458701.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(1173..2315)
                     /locus_tag="KPK67_001716"
     CDS             complement(1173..2315)
                     /locus_tag="KPK67_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2458702.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(2333..4087)
                     /locus_tag="KPK67_001717"
     CDS             complement(2333..4087)
                     /locus_tag="KPK67_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBG2458703.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDCAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFARYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            4223..4429
                     /locus_tag="KPK67_001718"
     CDS             4223..4429
                     /locus_tag="KPK67_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458704.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(4434..5357)
                     /locus_tag="KPK67_001719"
     CDS             complement(4434..5357)
                     /locus_tag="KPK67_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458705.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRIVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(5611..7311)
                     /locus_tag="KPK67_001720"
     CDS             complement(5611..7311)
                     /locus_tag="KPK67_001720"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBG2458706.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIMLAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(7570..7983)
                     /locus_tag="KPK67_001721"
     CDS             complement(7570..7983)
                     /locus_tag="KPK67_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG2458707.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITAKLLEEEGIKVKSEKDL"
     gene            complement(8051..10429)
                     /locus_tag="KPK67_001722"
     CDS             complement(8051..10429)
                     /locus_tag="KPK67_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG2458708.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKSGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSFMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTHKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            10747..12198
                     /locus_tag="KPK67_001723"
     CDS             10747..12198
                     /locus_tag="KPK67_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBG2458709.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKKVEGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(12402..14915)
                     /locus_tag="KPK67_001724"
     CDS             complement(12402..14915)
                     /locus_tag="KPK67_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG2458710.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPLEGVRTV
                     AKFSSKPFYFITPKGSTDLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIVMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEDCIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(15138..15959)
                     /locus_tag="KPK67_001725"
     CDS             complement(15138..15959)
                     /locus_tag="KPK67_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2458711.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(16477..16953)
                     /locus_tag="KPK67_001726"
     CDS             complement(16477..16953)
                     /locus_tag="KPK67_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBG2458712.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(16987..17565)
                     /locus_tag="KPK67_001727"
     CDS             complement(16987..17565)
                     /locus_tag="KPK67_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG2458713.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(17669..20269)
                     /locus_tag="KPK67_001728"
     CDS             complement(17669..20269)
                     /locus_tag="KPK67_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG2458714.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
BASE COUNT         6483 a         3674 c         2288 g         8087 t
ORIGIN      
        1 atacttaact atacttaaaa agcatataaa ttgatataat tatataataa ataagttaat
       61 attgaggagg gataattatg gaaataagag tattaagata ttttttagca attgcaagag
      121 aagaaagtat atctggtgcg gcaacctttt tacatataac acaaccaaca ctttctagac
      181 aaattatgga acttgaagaa gagttaggag ttaaattatt ggttcgagga aatagaaaaa
      241 tcaccttaac aaatgaaggt aggcttttac ataaaagagc agaagaaatt atatcattag
      301 ttgataaggt tgaattagaa tttgacaatc ccaatgagat tattagtggt gatatatata
      361 taggaagtgg tgaaactgaa gctatgcgac ttatagctag aactgcccat cattttcatc
      421 aaaaataccc caatgtaaga tatcaccttt ttagtgggaa tgctgatgat gtgactgaaa
      481 aattagacaa gggactgctt gattttggta ttttaattga acctgcaaat atacagaagt
      541 atgattatgc acaaattcct gctactgatg tatggggttt actaatgcgt aaagatagtg
      601 cccttgcatc acaaagcaca ataaatccaa aagatttatg caatatacct cttattattt
      661 caagacagac acttgttgat aaagcaattt ctaaatggct tcaagacagt aatgaaaaac
      721 taaacattat tgcaacatac aatttagttt ataacgcttc tcttttggta gaagaaggtc
      781 tgggctacgc tctgtgttta gatagactcg tcaatactac agaaaacagt aatctttgtt
      841 ttagacctct atctcctcct ttagaagcta aattaaatat tatatggaaa aaacatcaaa
      901 tattttcaaa ggcagcagaa aaatttttac ttcaaataca aaaagatttt tcttctatca
      961 tgttggatac atctaagttg gatacaccta aagaaaaata aatcataata aaaagtgaga
     1021 gtgtctcaaa atgaactttg cactgtataa ggcactcttt tttatacaaa gtaaaatata
     1081 tgttcatcat ttcatcacta aaaaggaggc ttatctctat tttgagatag ccccacttaa
     1141 ccaataaaat taaatacaac ttaacaatat ccttacttat ttattccaag ttctcttaga
     1201 caagcaatag taatttcatc ttgtggattt aattcataag ctctttctat atagtaagtt
     1261 gcatcatcaa tatcaccatt atttaaataa gccattccta aattgcaaag tatttctggg
     1321 tcttccttta tcttagccgc ttgttcaaaa tactctattg ctttatctaa attattctta
     1381 taagcctcac aaagccccaa ttccactata gtatccacct gattaggctt tattatcaat
     1441 atcttctcaa gatacatctt agcctgttct atttcgccca tacccttata tccaagtgct
     1501 atcataaaca gcaagttcca ccaatccata tgttcttctt ctaatggaag taacttctct
     1561 aatccttcct taaacttacc ttggaataca agtgtatatc cttcttcata ttgaacttta
     1621 aagtccatct tgcccagatt ctcttgtacc tcagctacta aatcagcatc taaccctagc
     1681 cttaatgctt cttcccaagt cagcttagtc tttaaatatt gaccttgatt ataataatgg
     1741 tagcctaagt gataataacc taatgcaaaa ttctcatcaa catcaattac cttttcaagc
     1801 ttagccatgg cttcaagtaa gaatgcattc attgccttat catccatatc tttttgatac
     1861 tgtttagcta tctcttggca aacaatcgca tagttataca ttgcatctaa atcatctgga
     1921 tacattgtta taaatgcttt tatatatatt aaagaatctg tatgttcacc aatctcaaag
     1981 tattttctcg acatataccc catatacgat ttcaaatcca aatcaatatc ttttgataaa
     2041 gcatttaaaa acttctcata ctctgcattg tttttaaact tactatctat tccaattata
     2101 tatatcattg catctgctat agacattgaa tttaacttat cttgagcagt cttttctttt
     2161 atccctttta ctaatacctc attttttata ggtacatcta atccaccttt tggcacttca
     2221 tagcttttta atttaaactc tccaccatgt tttatagtta taaaagctaa ttcttctgct
     2281 ttttttagca aatatttttc tatcttaaaa tccattttat cctcctaaat acttaaatct
     2341 ctactttatg agttttagat ttctcaaata tttttttatc atcatactca aataagacta
     2401 cattttctac ttctcttatt tctgtattta tatcattttt taaatccaca acattaaatc
     2461 taaatggctc aaaatgaaca tattttatta tatgtttagc tggtttatca acaaaactag
     2521 gtaaatatgt tgataagtta tcattatctt gtaaaaatac atggcatcta ttcttaaaat
     2581 catccacatc tagcttatta aaaaactgtg gtatatttga tgttttaccc agtgatatat
     2641 agtcatattt taatatatca ttgaatacat cattatctat gttgattttt tcattataga
     2701 aatccatcaa tattttatat aactgattct ttccttgtgc caaatcaaag tagccatttt
     2761 catcaaaata cctggcaaaa acctcaaaga atttaaaagg gcttttttta taaaatctgc
     2821 ctataatata tctcatagat aacacaaagt ttttagaatt atagtatctc tccagtatat
     2881 cttctatatc ctttagcttc aaagtttctt catagcttat ataattattg tataaaaact
     2941 catatggagc acaatcctta tatttaaaac catgctcatc tgcaactttt ctcatacctg
     3001 tccctttaat catctttaaa aagcctaatt gcaaatgctc tatacctaga ttaaatacat
     3061 cattaaaaga attttcaaag ctcttataat cttcataagg cagtcctgca attaagtcta
     3121 agtgctgatg tatatttcta taagatgcta cagtttgtac aacatgagac aactttttaa
     3181 aatcatccct tctacctact gcatcaagtg ttttttcatt cgttgactga acccctattt
     3241 caaattgaaa aagaccttct ttacaatcag ccaaaaactc aagcatctta tcatctatca
     3301 aatacgctgt aacttcaaaa tgataagttg tatatccatt atcatttttc ataaggaaat
     3361 ccatgatttc catagccact tttctatttg cattgaatgt cctatctata aactttatct
     3421 gtgatactct tgcatctatt aaagctttta aatcactctt tatcctgtct aaactaaaat
     3481 accttagacc tggaatagta gatgataaac agtattggca gttaaaaggg caacctcttg
     3541 atgattcata atacactatt ctattttcat attcttttgg attcaagttt tcataaggac
     3601 taggaatctc atccaaatct tgaagtaatt ccataggctt attcactatt atctcattgc
     3661 cttgtctata tacaagtcca tttacatcat ttatttccat ctttccttgc aagtgaagta
     3721 ctaagtctct aaaaattaat tctccctctc cataaagtat ataatccaca aaatcatact
     3781 ttttcattgc ctcataacta tcataactca cttccggtcc gcccaaagct attttagtat
     3841 taggtctaat tttttttaga ttatcacaaa gtttaactat atctcctaca ttccagatat
     3901 atgttgagaa taatattata tcatattcat ttttatatat atcctttaaa atatagtcca
     3961 aatcgttgtt tatcgtgtat tctttcatat taacttctat caaatctctt acaaattctt
     4021 ttagatatct tatagccaaa tttgtatgaa taaacttgga atttaatgtt gttaataaaa
     4081 ttttcaaata tttctcacct ctttttaaga ttatactgtc tttttgtacc ccttataggt
     4141 taaaatagta tatgtaaagt ctaaataata tctatataat taatttatta atttcaagta
     4201 atgtgaggaa aggaagtttt ttatggaatt aattgaaaaa gtatctatca actctttatc
     4261 aaaaagagat ttactactta ttataaagtc tcttgaatac actaatgaaa atactcaaat
     4321 tagtgatttc attgacctta ggaataatat agtaaaggaa ttatgttttt taactgacac
     4381 taaggaaaaa gattttttag attatctaga aaaaaatagc acactctaat aacctaaacc
     4441 tttttaaact ttaatatata aatacctgat ttagaattta tttttatgct taatatatta
     4501 tcattaaagg ttttcgacat aactgttatt tgattaaata tagggtctat tatagtatta
     4561 ccttcttcaa gtcttataag acttcttggg tctatgcttt tattatatgc cttaactcca
     4621 attttatatt ttctcaattt aacaacatat tcggacatag aaaaaataaa atctatatta
     4681 tcttcattta atttactatc ttcacccaaa atggataata tagatttttt tatgctatta
     4741 attccttcat tttctacaat tctatcttcc atatatacat catatagaac atttaaaaca
     4801 tttaataaaa tactttcttc atcataatct tcagatttca aatcaatgta cttatcaatt
     4861 aattgtattg ctttaatcct tgccatctga aactttacaa catctgattt gtctaattct
     4921 atagaaaatc ctattatctt gtctttaata ctctgtagta attcgacata ctctatttta
     4981 ctattattta taagattgtg cataatggaa ttatacacca cagaacctaa taaataatca
     5041 aataaaagtt cttctttttt aaaataaata acatactttt ttattagttt aataacctca
     5101 tcttcacttt tttgaatatt agtatttgat aaaattagtg gtaataaaat atttaaaaaa
     5161 ttgatatttc ctattttttc tccataaaat ctatagtaaa taagttcatc tctcagtcta
     5221 ttttcgtcta tatacacaca tctttgtgtt ttatcatcta ttaaggattt tgtatttatt
     5281 atagttaaat ctttaagagc cctacttgca ctactaaaag aagatgtgta gacttttttt
     5341 agtaattcgt cattcataaa tatatccttt ctgaaaaatt gaatgtaaat aaattgttat
     5401 taaaaaaaaa gagacagccc gtttaagaag ctgtcatata tagggggata gtagtgtaca
     5461 aaaatcattg taatctacta tctatatatt attatacgat actacttttt tatatgtaaa
     5521 gcatcagttt tcgacatccc tatattttac agttatacaa attgcgtact aagttcaatt
     5581 ttattctttt tatttcaaca ttttatcatg ctacattctt tcaggagcgt gcattcccaa
     5641 caatttcaga gaattttcta aagtttgttt tgttgctaaa actaatgcaa gtcttgcttt
     5701 tcttatttcc aaattttcta ctaaaattgg attgtcatga taaaatttat taaacgcttg
     5761 agctaaatct agcataaatc tagtcactat atgtggctca tttttcttta gagcaagtaa
     5821 aatattctta ttgaatgatt ctataactct aagaacttct gcactatctt catcagctaa
     5881 tagactataa tctatatcag aagtcacttc tacttctgct tttcttaaaa ctgcacagca
     5941 tctagcatgt gtatactgaa catatggtcc agtctcacca tcaaaactaa gcgttctttc
     6001 ccaagagaag gtatagtctt taattctact attagataac tcttggaata ctactgcccc
     6061 aacacctact tgcttagata tttcatccac atttttagca tttgggtttt tagctagcat
     6121 aatttcctta gttttatcta tagcttgatt taaagcatct tctaaaaata ctactcttcc
     6181 ttttctagta gacatagtac cttcttcaag tgcaaccata ccaaatccaa catgaattaa
     6241 atcttttgcc caatcatatc ccataagctc aattacctta aaccattgtt ggaaatgtaa
     6301 gttttgttga gaaccaacta catatataca cttgtcaaaa tcataagttt cttttctata
     6361 tattgcagca gctaaatctc ttgtcatata taacgttgaa ccatcattct tagtaataag
     6421 tgctggtggc attttatact cttctaaatc aacaattctt gctcctttag actcttttaa
     6481 aagatttttc tcttctaata gttcgataac tctgttcatt ttatcagaat aaaaactctc
     6541 tcctgctaaa gaatcaaatt ctatatctaa aagtttatac actctgttaa actcttttaa
     6601 actctcattt ctaaaccatt gccataattc ttgtgcttct tcgtcaccat tttctaattt
     6661 attaaaccat gctcttgctt catcttccat ttctgggtgt tgttcagctt catcatggaa
     6721 tcttacgtat aattttagta attctggaat tggattagac tctactactt ctttttctcc
     6781 ccacttttta aaggccacta ttaacttacc aaactgagtt ccatagtcac ctaaatgatt
     6841 tattcttata gtcttgtatc cttgcgaatc atatatctta tataaagcat tacctataac
     6901 tgtagttctt atgtgcccta tatggaaagg ttttgctata tttggagaag aatattcaac
     6961 tattacagtt ttatcttttc caaaatcact atgtccgtaa ttttcttttt catctaaaac
     7021 ttttttaatt acagtttctg ctaattgaga tttatttaca aagaaattta catatccgcc
     7081 taattgtatt acttttgtta tctctccact tggttctata ctttccgcca attcacttgc
     7141 tatcatgttt ggagcttttc taaacacttt ggctaattta aagcatggaa aagcatagtc
     7201 ccccatatcc ttatttggtg gaacttctat aagtgcctct atctcttctt tcgataaatc
     7261 ttctattttt tctttaaggc aatttgatat tgccacctta aaatcttgca ttctgttgct
     7321 cctctctaca ttgttcctgg tttatattgt gcttatctct ttatgttaaa tcaaatttcg
     7381 tgttttttca agtatttcat tacagtgtaa aacactttaa taattatgtt ttctgtatat
     7441 attaaaaata ctataattgc agtttttaat cacactatta ttgatttttt ataagacttt
     7501 tttctatttt attgtttctt gtactatcca gttgacttta tatacttttt ttaatctttt
     7561 atctattttc tataaatctt tttcactctt aacttttatt ccttcttctt ctaatagctt
     7621 tgctgttata cctttccctg gtatcttttt acctgaaaaa ctaccatcat atattagatt
     7681 acatccacaa gaggggctac cctcttttag tattgcttcg ctacatacat ataaatttgc
     7741 tatttttaga gtttcattag cccctcttac aaagttttct gttacatcct tattatcttt
     7801 agtaattact tttccatcaa ctatctcagc aggtggtctt ggtgtagata gaccacctaa
     7861 ctgctctggg catacaagtg taaattcttt gccttttaaa tattccttga ctttttcatt
     7921 atcattatta tcgccactat atttacaatt tattcctatt aaacatgcag ataccaatat
     7981 cataaaaatg tgctcctata caaattctta atatattctc atacaaatta ctaatgtatt
     8041 cttatacaaa ttattttaat ttaacaattg ttactcctgc tccaccttct ccatattgac
     8101 cacctctttg tgattttaca tgtctatttc ttcttaaaat gtcttgtagc cctgctttta
     8161 atactcctgt acctattcca tgtattactg ttacagactc taaacctgcc acatatgcat
     8221 catcaagata cttttctact tccattatag cttcttctag gtttaaccct ctaaggtcaa
     8281 cttcattttt tacactacca gattttgacc ttataatgtt tctcgttgat ttagttacat
     8341 ttgttgaaac atctttgtgt gttttttgta aagatttaaa aggtagagtc atcttcatta
     8401 taccaatttg cactactgct tcttttctct ttttatctac tgaaactaca ctaccatttt
     8461 gatttaaagt tattacctta acttcttcac ctggctttaa gtcttttatc tccttatttg
     8521 atacctttgg aactatcata ctttttactg ttggctgaag acttcccata aagctggtca
     8581 attcttttct aagttcttct atcttacgat tcttttcttt tgaagctctt tcttgttcaa
     8641 gacttctaag ttcttttatt ataatatcta cttcttcttt agcttgtctt ataattgaaa
     8701 atgcttctga tttagctttt tcaatcatct tatctctttg actgaccaat ttttcaagct
     8761 tttcgtcata ctctactttc agtttttcaa tctcttcttt taatctttca gcttcttctc
     8821 tatcttctac tgctttgatt ctattctttt caacattctg taacacatct tctaaagcta
     8881 tgttttctgt atttatatat tctttagcac gtgaaatcac ataatcactt agcccaagtt
     8941 ttctagaaat ctcaaacgca tttgattttc cgggaacacc tataagtaac ttatatgttg
     9001 ggcttaaagt ttctatatca aattcaactg ctgcgttttc tacacctgat ttagtaagag
     9061 cataattttt aagctcacta tagtgagtgg tagctataca tttagctcct acagaattta
     9121 tatcttctaa tactgctatt gcaagggcag ctccttcaac agggtctgtt cccgctccta
     9181 actcgtcaaa tataactaaa gagtctgctg ttacgttttg taatatagat actatattcg
     9241 tcatatgaga tgaaaatgtt gatagactct gttcaatact ctgttcatct cctatatctg
     9301 caaatacatt atcatataca cacatactac ttccataatc agcaggtata tgaagtccac
     9361 tttgtgtcat aagtgcaaat agacctacag tttttattgt aacagtctta ccacctgtgt
     9421 ttggacctgt tatcaccaat gtatggaagt ctcttcctaa ataaattgta tttgctacta
     9481 ccttcttttt atccagcagt ggatgtcttc cattttttat gtttaaatat ttatcctcat
     9541 ttaaagttgg ttctattcct ctcatctgta tagaaagttt tccctttgaa aatgcaaagt
     9601 ccaatcttcc aagtatctct tgattagaga ttaaatcttc gctcacttct ccaaccatag
     9661 cagaaagctc tgataaaatt ctttctattt cctcttgctc tcctagtttc aactgcctca
     9721 attcattgtt catctctact atagtcattg gctctataaa aagtgtcgca ccagaagatg
     9781 actggtcatg cactatacca gctacttgcg acctatattc agattttaca ggtactacaa
     9841 atctatcacc tcttagagaa attattgcat cttgtaagta cttttgataa gtagttgaac
     9901 ttattattga atttaatttt gacctaatgg attgattttt ttgggctatt cttcttctga
     9961 tatctctaag tgtacttgaa gcattgtcag atatttctac ttcactaatt atagcattgt
    10021 atatctggtc ttctatgtcc ttgtatacat atagagaatt tgatagagat tgtattattg
    10081 gataattaaa atcttcttca tcactactag ataagctatt tcttaatctt ctagccactc
    10141 ttaaggtatc tgctatcata atcagacttc ctgggtcaag agaagcacct atataagctc
    10201 tctttacctt atcttctatg tcatgaatac cttccaaccc tactgaacct ctctttataa
    10261 taattgattg agcttcacta gtttcttcta gcatagattt cacttctaca aaatcagtat
    10321 ttggtactaa attttctata tattttagac ctaaagaaga tgaggctttc ttctttaaca
    10381 aatctattat tttattgtat tctagaactc ttaacgattt ctcattcatt ttctatattc
    10441 accatcctcg atattttaac ttttttattt aattttaaaa atgtatgttt tcaagcattt
    10501 ccaataaaaa ctgcaagttt tatttctttt tcatttctaa aattattata ttttttctct
    10561 tttagatata ttttacacat attttgtatt atactatgta tgtagctatc tatatattat
    10621 acataaacat gctttatttg taatataatc tgttaaaaaa tcttctttaa tatttttaaa
    10681 ggagtttatt ttagcttata aaaaaaatag ctacttatat agataaatta aggaggaaca
    10741 tgagtcatgg gaaacgtatt agaaggatta aaaccagaaa gtgtttttaa aaactttgag
    10801 aaaatatctc aaattcctag aggttctgga aatgaaaaag gcataagtga ttttttactt
    10861 agctttggta aaaatttagg tcttgaaact attcaggatg aaagcttaaa cataataata
    10921 agaaagcctg ctacaaaagg atatgaaaat tgtccaggtg tagttcttca aggacatatg
    10981 gatatggttt gcgaaaaaga aaagaatgtt gaacatgatt ttcttaaaga ccctataaaa
    11041 ttaagaattg atggtgacat gatatatgct actggaacta ctctaggagc tgacaatggt
    11101 atagctgttg ctatgggtat ggctatatta gaagataata ctttagagca tcctgcacta
    11161 gaagttttag ttactgttaa tgaagaagat ggtatgaatg gtgcagatgc actagaccct
    11221 agcttaatta aaggtcaata tatcctaaat atggactccg aggaggaagg atacttatta
    11281 gttagctgtg ctggtggtaa aacttgtgtt gttagtttac ctgttgaata caaaaaagtt
    11341 gaaggtgaca aacaaggatt attagttgag gttactggac tattaggtgg acactctggt
    11401 atggaaattg ttttacaaag agcaaatgct aacaaagcaa tagctagagt attaagtgta
    11461 ttgaatgttg attatgaatt agcttctgtt gatggtggta ctaagcacaa tgctatacct
    11521 cgtgaagcta aatgtgtaat agctgttaat aaagctgatg ttgaatctgc taaaaaacaa
    11581 ataaatgaca ttttaactgc atttaaacat gagtttacta cttctgaccc tggcatgact
    11641 tactcagtag ctgaaacttc tgtagacaaa gttcttacta aagattgtaa agaaaaagtt
    11701 gttcaaatgt cttgcttaac tcctcatgga gttcaatcag taagtcttga tatagaagga
    11761 ttagttgaaa gttctactaa cttcgctata attgaaacta aagaatctac tattgaattt
    11821 ttaacttctg ttagaagttc tgttatgtct attagagatg aaatcgctga tagaataaga
    11881 cttttggctc aagctttagg tgctaattac gaccttatag ctcaataccc agcttgggaa
    11941 tttaaaaaag gctcaaaatt agaaaaaata tgcagtgaaa cttatgagaa attaactggt
    12001 aaagttccta ctgttatggc tctacatgct ggtcttgagt gtggattatt acttgataaa
    12061 cttcctcatg ctgaagctat ctctatagga ccagatatgt ttgatgttca tactccaaat
    12121 gaacatgtta gtatcccttc tgtagctaat gtttgggatt atgttattga aatattaaaa
    12181 tctatgaacc aatattaaat ttaagttttg attctaagta gattttaatt ttaagtaatc
    12241 tatataaaat taactctgta aattaatact actgttagta taatatttac tttcaatatt
    12301 aacttataga aataaagcct aatgatagat aataacatgt atcattaggc tttattgtaa
    12361 tttaaatatt attaagcttt attctgtttt aaaattaatt cttatactat tttaaatcta
    12421 tttcttcctt gtctcttaga atcataaaga gcattatctg ctatttgata cagttcatca
    12481 tactcttgac caggctttgc tataactacc cccatactaa tagataaact ttctccattt
    12541 ggcaacttaa tgagatgaat atgattgtat acatcattac atttattaat aagttcttcc
    12601 ttatttatta tatcattgat ataaacacta aattcatctc cacccaaacg acctactata
    12661 tcattttttg caaaactttc atcaagtacc tttgcaacat caataagtac tttatctcct
    12721 aacaagtgac cataagtatc attaatcgac ttaaaataat caatatctat aatcaataaa
    12781 gcaccatttt tttcagttga taaattcaac aaattttgtg cagttaattt tctaatatat
    12841 gctgcattat aaatattggt taaactatcc ctttgagcct tatctaacaa tctattcttt
    12901 tcctcttttt cagaatcaat attacatatt ttcccgatta catacattgg tatttttcta
    12961 tcattataaa ttatttttga agtaactcta atccaatgaa aagtatcatc aagcataata
    13021 gaatacattt cagttgttcc attttcttgt gattttatta catcaaaaaa ctttttatta
    13081 tattctttcc ttattttatc tctttcattt tctatattgc cattttcact ttccttatca
    13141 tttttatcca aaccataata agtctcaata cccttttctg gcatagaaat tatcaattta
    13201 tcttgcttat aatcatactc aaaaaagtat tcacttgtca tttcatacaa ctgagtatgt
    13261 tttttgagtt ctaaagatat cttctcgttc atacttgtac gagtatgcaa accaaatgcc
    13321 ataaatccaa taatacaaat taataaactg gctacaataa aaatagtctc aattggattc
    13381 tgttgcataa tataaagcaa cgagaagtct tgtctatatg tagtattttg gtagacaatt
    13441 gattgcattt tttctgatga aatacttata atagctttat taaatatatt caataattct
    13501 tgtttatctt ttttaattat accaaaacaa atactctttg ctttataagt ttgaggaact
    13561 agacgcacat ttttatactt aggttgattc atatagtact gcatagaata accttcgcca
    13621 taagtgtaat ctgcatagcc tttattaaca gcttctatac aatcttctaa attatcatac
    13681 caaactatat tctcttcaaa ctgaccataa taattaatac ctttggctag agctacacgt
    13741 tttccatcta aactattctc atttaattta ttattaatca tcatagtata ttgagtagaa
    13801 agaaaaggcc gagacataac tatattatgt tcacgagagt attcataatc ataaatcatt
    13861 cctgctacaa tatcaatttc acgcttttta gtcatgtttt ctaaatcttc ttgagatttt
    13921 acaggcacaa gttcaaattc taagcctgtt ttatcacata tatgatttaa caaatcaatt
    13981 gatatacctc ttaattcacc tgtatcttta tctatatttt gaaatggtgg tttgtttaaa
    14041 agtactccta cttttaattt ttctgtctct ttaatgtaat cagtttcttc atcagataaa
    14101 aacatttgat tattctctgg tgtaaagtat ttttcatata aagtagttga aaaatatggg
    14161 tctttctgtt caatattatt aattgctgag ttaagctttt gagttaagtc tgtactgcct
    14221 tttggggtaa tgaaataaaa tggttttgat gagaattttg ctacagtacg tacaccttct
    14281 agtgggttca tactcacatt aagaagtaca tctgctttac cactctttag tgcttctagt
    14341 tgctcatcat ttgttttaca atataccaat tcaggtgaca tcagattcat cttacaatac
    14401 tcatctaact ctttaagtct agtatcagaa ttttctatta cagcaatacg gatttttttc
    14461 acatcttgtg aactcattat gttatcaata tctgtatttt catataatgc ttgtagtact
    14521 gtatttgcta ctccataact atttcctgca taattatata ttttttgcat tggctcattg
    14581 tataacatac ctcccattat atcaatttca ccattttcta acatcttcat taattttgta
    14641 atagattcat ttatctctcc aggaacttga acaaattcat aattccatcc agtatactgt
    14701 gcaatttcta gtaaatattc atatgtatac ccaaaatagt taccttgctc atctatttca
    14761 gttaaacctt tttgaatagg ataagcaaca cgaatggtac gattatcatt acctatagcg
    14821 ttgataccaa aagaaaatat gcaagaagtg aatatagatg taattacaat aattaataaa
    14881 agtaattttt ttaaattcac tacacaatcc tccatatagt taagatttac agtataaatt
    14941 actgcataaa cttgtagaca tgtaaaaata gattagtata taaaaggata tataaatcta
    15001 cttagaaatt taaatatcct taatatatac aatatattta aattataact catttatatg
    15061 tctattacaa caaatttacc ctgctaaata cctactctat atcccataat tttacaagtt
    15121 gtagtgaagt ttaagttcta tctcacctca aatttagcag tgtttctatt tacagttctt
    15181 ttatatttca catctggata acccaatata aatgaagtga taacagcttc gttctcgctc
    15241 attcctaaca gttcttttat ctttggctct acatttgatg ccataacaaa aaatccatta
    15301 taacaaattc ctaatccttg agaataggcc ataagttcca tattagatgc agccagtcct
    15361 ccatctacat aagcacttcc acctagagac atatcaccta caactaccat aagtagtggg
    15421 gcatcataaa ataaagaatc atttccgttc tctttatatc ttttgtacat tttcttaaat
    15481 gtatttttat agatagaacg tacagggtca ttgtcatcag tatctaatgc caaattataa
    15541 agaccttgta ttgccagttc tttcacttct tttagctttt cttttactaa tatgtatctt
    15601 ataggctgtc tattaccacc tgttggtgtg tatctaccag cttccactat gttttttatt
    15661 ttttcttctt ctacctctat atctttatat tgtcttatac ttcttctaaa tttaataaaa
    15721 ttcaataact tttcgggttc taattcaaaa ctttcacgat tgtattctat tacttcattt
    15781 ttatcaaatc cttgcatttc tattgcatct gttgggcaaa ttgccataca gtgaccacag
    15841 ttaatacaga gaatattttt aacctttgct ttatcatcct taacttttat tgcatttaca
    15901 agacagtctg attcacacat cccacagcct acacattttt ctaaatttat gtctatcatt
    15961 ccaaattatt cctcctttta aaaaatttac tttagtataa aacaaatagt tgtctaagtc
    16021 aatttttatt tttttaacaa tatactagcc ttaactacac tttaatggag aaaaatatac
    16081 ataaattgga acttaaatta gaacttattc aaagtaaaag ataaaagaag tagatgtcta
    16141 aaagtgaact ttttagttaa ttagatattc tttattgtat gaaatcaagt ggatatttat
    16201 catttcaaca ataaaaaagg cttacctcta ttttggatat gtccctgtca agtagacata
    16261 ctaaaaaaag gctatcttta tgcactgaga tagccccttt tcccaactac tattgtttat
    16321 cttaatatta gtcaataaat ttaatttgct ataacctgta agttacattt taatttgatg
    16381 taactttaaa ttacattttt aagtaatatc tttaaattat attcttaaaa tatatcatta
    16441 aactacattt taattaatct aaaaactata tcttaattaa tctaagaaac ttacattata
    16501 atcttttagt ttcccattat catatacaat ctttgcacta aagatatttc cagtcttagc
    16561 ccaatttaag aacagcctgt caccttccca catatttaaa tcgaatattt tatcattatc
    16621 aacccaaatt aaatttcctt ctttacaatc atttaaatct ttttcagata attcaccttc
    16681 aaactcatct gagaaataca aaaacatata ccaatcttct tcatctttaa attttggaaa
    16741 agttatcaac cctttatatg acaatgattt aagagtaaga ccagtctctt cttttacttc
    16801 tctaattaca cattcttctg gagtctctcc ttgctcaaac tttcctccaa ctcctacata
    16861 cttaccttca tgtatatcat ctttcttttt atttctgtac aacatcaaag tcttgtcatc
    16921 tttttctata taacaaatag ttgtaagttt catgtatctc gccttgcttt ctctttatat
    16981 tctcaactaa ttattttcag cactttcaag cacatcactt atattcttca ttgcattaag
    17041 agaaagctct gcaaattcat caaatggaat ccctatatct tctatacttc tcattgcatc
    17101 tctatttaca tttgaagcaa atctcgtttc tttcattctt ttaataacag acttagtctt
    17161 tacactggta agttttttat ctggataaac taaagctata gcagttataa acccagttat
    17221 agggtctgct gcatatattg ctttttccat taggctagtt cttgtaactc ccattccatc
    17281 atgatgacca agtattgctc tatacatttc ttcgtcaccg aagttttctt ttttaagcag
    17341 ttctactgtt gttgtagcat gtccttcaca tatttctcct ggctttcttc caactatatc
    17401 tgaatccaaa tcatgtaaaa gacctgccac tgcccactct tcttcttttt ctggctctag
    17461 tcttttggct aattctctca taacagcttc aacagcataa caatgttttc tcattctatc
    17521 agtttctaaa tacttaaata aaatttctaa agcttcttct ctcattttca ccaatccccc
    17581 tgttttttca catatatgat atttttatat attaatacac ataaattcaa gcttttatat
    17641 atattcatgt atatttttca tgttatatct attctacacc tttatagtag tgtcctgcaa
    17701 ctgttccaat ttcatctaat ttcttataca agtctgtagc cttttttcca agtttataat
    17761 tattactcag tacctcaatg tgagcttcaa taaccttttt aaccatctca ctatcttcaa
    17821 ctgtaaaatc tatcctaaat acatctattc caatttcctc taactcatat agattgtcca
    17881 acatacaagt aaccttcgaa ttgtaaattg tacttctaca gaatatatct tgtgttatcc
    17941 tataacttgc atccgtaaag tctcttaaag cataactact ttccttacat ttagcaactc
    18001 tcttgtcctt tttacaatcc ctaacaacta ctcccatagg acagtattct gtaaccatca
    18061 taggagcata tccatatatt actgcttcaa tttctacatc tgtatatttt aaagtttgtt
    18121 taatttcatt taagtttagt tcttgtgaca aacaaactgt acttgctccc tcttctttga
    18181 agtgatttat actttcacta ttgaatgcat ttaagtagca atctatattt aagtcttttt
    18241 tcttaaagta ttccatagag ccccagttac caatttgaac cttattaatt ccttttatat
    18301 tatccaatat ctcaaattgc ttgtactctt tatttctaac aatccttggc gctgaataga
    18361 ttaatttttt gtcagtgact gctgtattat agtggttctt attcaaagta tctattttac
    18421 ttgtatttga caaagtatct atgttatttg tatttgaacc cattagttct ttaggagtac
    18481 ctaaagacga agaagtatct aaatccatag aaatacttga atctatagaa atatttgaat
    18541 ctatagaaat acttgagacc atttgtaaag cttttgataa tgtatttata tcttcataat
    18601 agattaaatc tacattataa tttaatactg cttctaattg ctccaagttc ttaactttaa
    18661 ctctaatctt aggagctgta tcaagttttt tctgtttatt tactctattt ggctggtatt
    18721 ttaatctatt cttcttaaat tttctatctt ttatctttat tctttcttca tctaaaagct
    18781 caatacaatc ccttctcatc tgatttaaca cacttatggg catacttaca ccttcatcca
    18841 ggtcaatttc tatctctttt aacttatatg gtgtatttcc aagcttttga atttgtgttt
    18901 ctattttttc tttacttaaa gccaccttta tagctttttc aactattttg tctccttcta
    18961 ccaccgattt atttccacaa aaatcactta taaaaagctc tggcttttta cctagtttaa
    19021 tactcacttt tgcacgtata ttagttttta tgaactcttt atcttgctga aatgtagctt
    19081 gtactctatc cattaattcg ctgtcagaag tcttatatat aagctgattt ctctttgctt
    19141 ctccaacaaa atctaattct atcaaatccc ctttatgtgc tattgtagtt atctctccat
    19201 cctttattat tcttccaata gtacctccac ctaagttaat tccatcacct ttcttaagcg
    19261 tattttctag cattactcta agtcttttgt tctttctatt ataatctaaa acttttccaa
    19321 tatatagccc ttgattatta ggtctattag agttcattat atccttacca acttcaccta
    19381 atatatatcc ttttgtaaac tttctattaa atatggtata caaattattt attatttcct
    19441 cagaaatact tatttttttc ttatctacaa attcattaat cgcttctcta tatcctgaaa
    19501 ccacagtagc tacatactct ggctttttca ttctaccttc aatttttaaa gataatacac
    19561 ctgcttcaat tattttatcc aaatcttcta tagtacttaa atctctagga ctcaacaaat
    19621 aatctccatt actttttact acttctcctg tatttatatc tatcaactca tacttttgtc
    19681 tacatggctg tgcacaccta cctctatttc cagacctatt gcctatcata ctactcataa
    19741 ggcattgacc cgagtaacat acacataaag ctccatgtac aaaaacttct atatctacag
    19801 ttgtattttc acaaatccat tttatctcat caacatttaa ttctcttgct aaaacaactc
    19861 tatcaaaccc tacactctga aggtatttta catcttctaa ggaatgtgct accatttgag
    19921 tgcttgcatg tagttcaaag tcaggtaata gtttttttat aagtcttgcc attcctatat
    19981 cctgtactat taaagcatca atatctatat cgtataaaaa ttttacatac tctacaaaat
    20041 cttctatttc atactgtttt atcaatgtat ttacagtaac aaagacttgc actcccctta
    20101 tatgagagta tttaacagcc tcaatcagct catttctatc aaagttattt gcagatgctc
    20161 tagcactaaa gtctttacca cctaaataca cagcatttgc tccgttttga actgctgctt
    20221 ttaatgaatc aaaagagcct actggtgcaa gtaattctat ttttttcata tttttctcct
    20281 ctatttatac ttgttaggtt aatataatta tatctataac ttaaatattt gtattaaatt
    20341 tatatatttc ttactatatt ttaaattctt caatcaattt atatattagt tatttatatt
    20401 tggttcattt gagttaacat atacattatt tactatattt agtatatttc tattaactta
    20461 tatattgatt actgtatttt aaattcttca attaatttat atactgctta ctaaatttaa
    20521 aacattttaa tt
//
LOCUS       DAEMHW010000056        20483 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEMHW010000056 DAEMHW010000000
VERSION     DAEMHW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20483
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.056"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(551..2155)
                     /locus_tag="KPK67_001729"
     CDS             complement(551..2155)
                     /locus_tag="KPK67_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2458715.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVVDNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDNGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDKNPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            2541..3467
                     /locus_tag="KPK67_001730"
     CDS             2541..3467
                     /locus_tag="KPK67_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG2458716.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(3587..4585)
                     /locus_tag="KPK67_001731"
     CDS             complement(3587..4585)
                     /locus_tag="KPK67_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBG2458717.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            4689..5126
                     /locus_tag="KPK67_001732"
     CDS             4689..5126
                     /locus_tag="KPK67_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2458718.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            5212..5799
                     /locus_tag="KPK67_001733"
     CDS             5212..5799
                     /locus_tag="KPK67_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG2458719.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(6099..6719)
                     /locus_tag="KPK67_001734"
     CDS             complement(6099..6719)
                     /locus_tag="KPK67_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBG2458720.1"
                     /translation="MEELISKKDLLNKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(6727..7494)
                     /locus_tag="KPK67_001735"
     CDS             complement(6727..7494)
                     /locus_tag="KPK67_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBG2458721.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(7535..8356)
                     /locus_tag="KPK67_001736"
     CDS             complement(7535..8356)
                     /locus_tag="KPK67_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458722.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(8713..10623)
                     /gene="phnW"
                     /locus_tag="KPK67_001737"
     CDS             complement(8713..10623)
                     /gene="phnW"
                     /locus_tag="KPK67_001737"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBG2458723.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPNFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEELSKCKGIAKSLSLDVYAQWDTMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(10902..11687)
                     /locus_tag="KPK67_001738"
     CDS             complement(10902..11687)
                     /locus_tag="KPK67_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG2458724.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(11904..12134)
                     /gene="dltC"
                     /locus_tag="KPK67_001739"
     CDS             complement(11904..12134)
                     /gene="dltC"
                     /locus_tag="KPK67_001739"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBG2458725.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(12153..13301)
                     /gene="dltB"
                     /locus_tag="KPK67_001740"
     CDS             complement(12153..13301)
                     /gene="dltB"
                     /locus_tag="KPK67_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBG2458726.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(13301..14815)
                     /locus_tag="KPK67_001741"
     CDS             complement(13301..14815)
                     /locus_tag="KPK67_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBG2458727.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDEKDFGDINVTDMDKLNDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(14936..16126)
                     /gene="dltD"
                     /locus_tag="KPK67_001742"
     CDS             complement(14936..16126)
                     /gene="dltD"
                     /locus_tag="KPK67_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBG2458728.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(16414..17433)
                     /locus_tag="KPK67_001743"
     CDS             complement(16414..17433)
                     /locus_tag="KPK67_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG2458729.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(17609..18148)
                     /locus_tag="KPK67_001744"
     CDS             complement(17609..18148)
                     /locus_tag="KPK67_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG2458730.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(18249..18929)
                     /locus_tag="KPK67_001745"
     CDS             complement(18249..18929)
                     /locus_tag="KPK67_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBG2458731.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(18958..20247)
                     /locus_tag="KPK67_001746"
     CDS             complement(18958..20247)
                     /locus_tag="KPK67_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG2458732.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         6761 a         3506 c         2097 g         8119 t
ORIGIN      
        1 ttatcaatct tgtatagtta ttaactttta tataaatttt tttatattat aacatagtcc
       61 tatatatcta ttatttggat agaattattt tttaaattag aattatttta atgaatgatt
      121 caagtaatag gtatgaaatt gaatctttta gaactgctta caaaaaatac tcaaagaaaa
      181 atagtaattt tctcagattc acaatatatt ttatatgaaa tgttataagg taatctattt
      241 aataaattta aatttgctat tatagattta gttccaaagt cttactgcaa agtaatatac
      301 atagtttttt aataaactaa taagtgcaat taataactac ttaattgttt aatatagtac
      361 atagataact ttaataaatc gatagttatt taaaaaggta gaatcaataa aattatactt
      421 ataaatcttc aatacaataa ttaaaattta ataaaaatat aattgttgat ttaactgtaa
      481 tcttatttaa ttctacctaa cttattatat aaaactattt tttatagatt ataaaatatc
      541 taactttcat ctacttcaaa gaatacaata cttccccttc tcttatagtc tgcaatactt
      601 taatatcttt tattttcata gggtcgattg taagtgggtt tttatctaat attatcaaat
      661 tagctaattt accttcttta attgaacctt ttttatcttc ttcaaaatat tgatatgctg
      721 catttatagt aacacccttt aaagcatcta atgggcttat cttttcattt tctcctattt
      781 gtataccttt ttttgtaatt ctattgacag cacaccatac tgtttccagc atattaggtg
      841 ctattactgg tgtatcctga tgaagagtgt aaattaatcc cttttctata gttgttttaa
      901 gtgggctaat cttaaacgct ctatcttctc caaggttttt gatatgaata tcaccccaat
      961 aatatgtgtg agctacaaag tatgaaggta tcatatttat tactttcata tcatcaatct
     1021 ggtctgctct tactgtttga gcatgtatca taactggtct tatattattt tcactacttt
     1081 gctcttttaa gtttaaaact ttctcaaacg catctatcaa ctggtctgct gctgcatctc
     1141 cattacaatg agttaatagt tgcatctttt cttttagtga aatatctata aacttttcta
     1201 cttcttcatc tttgtatata ggatatccac aataaccatt atctgaattt tcatatggtc
     1261 tactcaacca tgctgtctta ccttgtggag aaccatctaa gaaaatctta taacctccta
     1321 ttttaaatct atttatatat ttttttataa actccctatt attatctact acagacttac
     1381 tttttttcaa atttacatat cctacaacat ccatttttaa tttattttga tttgccattg
     1441 cttttaaaat gttaaattga ttttcttcca ttaaaccttc ttgagctgtt gtaataccat
     1501 agcttagata tatgttctgt gctttttcaa ttgaattaaa aatctcattt gatgatggtt
     1561 gttttatttt agatgctaca ttaaaaaatg cattttcttc taaatatcca tttggttctt
     1621 cactgccttc tactctacct atatgtccac cttctgggtc acgtgtttca ttggtaacac
     1681 caagctgttt taaccccaat gtatttgcaa ctcccatatg accagaagca tgtgaaatta
     1741 gtattggatt ttctgaagat gcactatcga gtacactctt atctggatgc ttattttctt
     1801 ctaaaaaatt attatcatat gaaaatccaa ttatccagtc tccttttttt atattattcg
     1861 actctttgaa atcctgtatt ttttttacta tgtctttaaa actggttgca tcttctaaag
     1921 gaactagctt tagagtagta gcaaaagcta tcaagtgact atgtgaatct ataaaagatg
     1981 gcatcaatgt cttaccttgt aaatctataa tttctgtatc tttatttttc aaagcaaata
     2041 cttcttcttt tgttcctatc ttttttatta ttttgtcttt tataagaata gcatctccac
     2101 atattgaatc ttccattgtg atgatatttc cattgtaata aattttttct ttcataaaat
     2161 tctccttacg ttgtctactt taataaattt ttttcattat tttctgctta attatattat
     2221 cttctttata aactttttct ttagagttta atcatataaa agttttattt ttaattattg
     2281 tttgccaaga agtaaatact attcattgta aataagacaa attaataggc tttatactaa
     2341 ttgaaaaaat attataagtt gttaaattta aatatctaaa ctcaattata tctgtacaat
     2401 tgtaattttt taaatcatga tgtattattt taaatataca gttacattat tattcacaat
     2461 ataagtatct tagttctcaa ataacatctc atgtaatcta ttcaatgcaa aaaatttaaa
     2521 tattttttag gaggtttttt atgagcaagg aaaataaaaa tgtaagaatt gctgttgtcc
     2581 aggcgtcacc agtcataatg gatttagaaa aaactgttga aaaagcatta ggattaatta
     2641 aagaagctgg tagaaaaggg gcaaatatcg tagttttccc tgaagcattc atacctgcat
     2701 atccaagagg tctttcattt ggatttgttg ttggaagtag aacaatggaa ggtagagaag
     2761 attggaaaag atactatgat aattctgtac cagttcctag tgctacaaca gatttattag
     2821 gaaaagcagc acaagaagca ggagtatatc tatcaatggg tataacagaa agagatggta
     2881 atgatataaa ttgtaccctt tattgcacaa acttattttt ctctccagaa ggtaagctaa
     2941 ttggtaaaca cagaaaactt aaacctacag gaacagaaag atgtatttgg ggagaaggag
     3001 atggaagtac tcttacagta gttgatactc catatggaaa aatgggttcg ttgatttgtt
     3061 gggaaaatta tatgccatta gctagaacag ctctttatgc taagggtgta aaactttaca
     3121 tagcccctac tgcagattca agagaagagt ggcaagcaac tatgaagcat attgctttag
     3181 aaggaagatg ttttgtaatt ggatgcaatc aatatgttga aaaaaatatg tatccaactg
     3241 accttaacta ctataaggag ctagatgcag aaccagaaat tatgtgtcca ggaggaagtt
     3301 gtatagttga cccttttgga aaatatgtag caggtcctat tttcaataaa gaagaaatgc
     3361 taatagctga tttagaccta gaaaaaatag tacttagtag actagatttt gattcagaag
     3421 gtcactattc aagaccagat gtatttgaat taattgttca tgaataatct ttttacacaa
     3481 acatctacat aaatcttttt acataaaaaa atgagatact cataagtaaa tttcaagtat
     3541 ctcatttttg cttaaaacct ataatcctaa ttataaattt attcacctat attttttcta
     3601 aatcaactac tgttcttcca acatgttttc ctgccattat tgaatctata ctatcattta
     3661 actcttccaa tgatactaca tttaccatat catctaaatc atctaatttc cattcatttg
     3721 ataataaatc ccatattttt aatcttaagt tcattggaca atccacagaa tctataccat
     3781 ataaagtaat tccctttagg ataaatggat atactgaact tgtaaaatca actcctcctg
     3841 cattaccaca agttgttact gctccacaat aattagttgt ctttattgct gttgctagag
     3901 tatttcctcc tactgtatct attactccag cccatcttgg tctaagcaga ggtcttcctg
     3961 agttatcatc cacttctgac ctatgtataa catctttaac acctaagttt agtaatactt
     4021 ctttttgttc ttctaatttt cctgttgaaa ctacaactga ataacctaat ttaatcaata
     4081 ttttagttgc aattacagca actcctccag taccaccagt gactaataca tctcccatat
     4141 tcttgtctac agaatctata aatttataca cagataaagc tgctgtaaac cctgctgtac
     4201 cataatccat tgcttctttt agtgaaagat ttttaggaag tttaactacc caactagaag
     4261 gaactcttat gtattctgaa aaaccaccag aagtattcat tcccaagtca taacctgtta
     4321 ctaaaacttc ttctccaatt ttaaaatcat cacaactact agattctact ataccagatg
     4381 catcaattcc tggtgtatga ggatactttc tcgtaacacc cttattacca tttgcagaaa
     4441 gtgcatcctt gtaatttaaa gaagaatatt ttacttttat taaaacttcg ccttctggca
     4501 atgtatccac ttctctttca actatatttc ttctaaaatt tccttcttct tctgtaatat
     4561 atagtgattt aaatttttta ttcataaatt tcctcccaat acatttagtt ttatgttaaa
     4621 ctaattatag tttataaata gttttatgtc aaactaattt aaatagatta aagattggag
     4681 aataatttat ggaagataaa tatattgtac atttcataag taaaacaaag gctagtatga
     4741 ttaaattcat tgaaaataaa ctcagtaaaa atggtttagg tgagttaata cctactcatg
     4801 gaaatatttt aactgcactt tatgaaaata atggaatact tacaatgaaa gaaattgcag
     4861 ctaaaatagg aaaagataaa tccactgtta cagtgttggt taataaactt ataaacctag
     4921 gatatttaga aagacaaaaa tgtacaaatg acaaacgtat cacgtatata aagcttacaa
     4981 aaaaggcact gcttatagaa gatactttta atagcatctc aacacaagtt aaagagacag
     5041 cataccataa tattacagaa aatgaaaaac aagaatttct tagaatttta aaaaaaatta
     5101 atgataattt taaaaatgct gactaaattg agcataatta tatctcctat tggaataata
     5161 aataaaaaga atcaaatatt gtaaaaattt attatgatag agaggtagaa tatgaattta
     5221 aaagatagta aaactaaaga aaatttatta agagcttttg caggtgagag tcaagctaga
     5281 aatagataca atatttctgc atctgtagca aaaaaagaag gattatatat aatagagtct
     5341 ttatttaatt acactgctga ccaagaaaaa tcacatgcaa aacaattctt ttctaaatta
     5401 aaagaatttg ctggtgaaaa tataagtata tcaggagcat accctgtaga tttatatgac
     5461 aagacagcac aacaccttaa agctgcacaa cataatgagc ttcaagagtg ggatgatgta
     5521 tataaaaact ttggaaaaac agcacgtgaa gaaggatttg aatctatagc taagttattt
     5581 gaaaatatag ctgaaataga aaaagttcat ggtgatagat ttggtagata tgctactgat
     5641 ttagaagaag gtactttatt cagaagaaat gaagatatac aatggatatg tacacattgt
     5701 ggccatatac atataggtaa agaagctcca aaagcttgtc ctgtatgtct tcatccacaa
     5761 ggatatttct tagattttaa aaactcatta tttgaataaa ctaaggcaat aattctattt
     5821 aaacaattta ctatatttat atcaagtatt tttataaata ctgtattatc ttaataacaa
     5881 taaaatgaac cattgatatt actataagta gaaaatgtag tttaaataca atttatagta
     5941 attcatcatg gttcatttta ttatttacta atccctataa cctacttaaa tctatcttta
     6001 acttttacat actcacataa aatcttatct ttaaactaaa tatttaaata aagtgtattt
     6061 taagtagatt attataatac attatataag ataatacact atgataactt tatacttata
     6121 tcatttattt cttttgtcaa gtcaatctca ataatcttaa ctacatcttt ttcaaacttt
     6181 acttttttat aatccagaca tcctactacg aatggaactc caaactttct aaacaaataa
     6241 atctttccac tttcattgta tattgatgta aaattttctt ctattatatt cactatcaat
     6301 tttaactctt caaaagttat tatagattta actaaaaact tctctattat gctcaatata
     6361 agcagttctt ttttacctat gctttgctct tttttatata aattttgaaa tatttctttg
     6421 gtataattac atataacttc tttactgaga ataaaatcaa tgtcaaattc tttattaata
     6481 tcctttttca taaacatatt tgcaatatca tcaagagaag tctcttcttt catagacaat
     6541 attaactcta ttctttctag tattttatct cttggaaaga aagtctcttg acctgtaaaa
     6601 gctgattttt ttataaacca ttcctctggt attatgtttt ttcttttcca tctatataat
     6661 tgaccataag atatattagt tttgttcaat aaatctttct tagatattaa ttcttccata
     6721 acttttctag cccaccttta tactgttttt tacagtagaa ttatctgaaa ctgataaatt
     6781 atcactgtat tcaatatttc caacgtcaca attatctact agagatatgc ttttgcctcg
     6841 tatatctttt attgatgcat cttctaattc aatagtatct ccctctatca aattacattt
     6901 aaactttcct gaaaaaatat taatgatttt aattaatgaa aaagcatctt tgcttcttcc
     6961 tatttttata ttagttgccc ctatctcttt acaataagaa cctcctcctg aataaacata
     7021 tatattttct ccatttaaaa gaccatcaca ttttattact ccatatacct ttatatcttc
     7081 acattctata tttgatttat ttctcatttc gccatagatt tttatatctt gtgcatacat
     7141 attttcattt acacttaatt gtcctcttat tgtaacatta ttacatttaa gtttgccatc
     7201 cactttctgt tgagcagtta taactaagtc atctatttct gcatctccct ctatattaag
     7261 ttgaccattc actcttacac gctctgcttt aagattgcct cttataatag tttttccgtt
     7321 aacaacaaaa ttttttatat tgatgtcacc ttcaaatttt gcatctccaa ggacttttac
     7381 ttcgttactt ttaacatttc cttttgattt agaacttccc acgattttta tattttcata
     7441 ctcaccacta cttataaagc cttctccaag tatttttata gacccttttt ccatattatt
     7501 acctcatttt aaattttatt tttaaaccta tttattactt atccttcttt aattttattt
     7561 tttcacttat attctttatt tcaccaacta ttgaatcttc atcaatctct atactttcac
     7621 tgtactccaa actttctaca taacatccct tgcctatttt aatatttttt cctcttacaa
     7681 ctttgacttc actattttct aagcttattt catctgcttc tataatattt gcaaacaatt
     7741 tactttctgt atactttcta attgataata gacctaaact tattaaattt gaagacactt
     7801 tttcaaagtt gatatgcttt ttttctagca tacgaaattt tttgcaataa tctccatgcc
     7861 ccttttttat atttatacta cttgctccaa cttcattaaa attagaagtt ccatttaaac
     7921 taatattaac ttcttcacaa ttcaaaaatc cattacactc caagattcca cttaaattga
     7981 tattttcaca atctatattc tcatttgctt ttaaatatcc ttctccatga ataatatcag
     8041 attttatact tccttctaca ttaataattc cactagtatc aatttcaaca gaattaatat
     8101 ttcctgtaca tctaagaagt cccgatatct caacttttga tgccagtatg tttccttcta
     8161 ctttacttaa accagatgta aaaaattcat tacattctgt tgtcccaaaa acttttgctg
     8221 aaccagaaga atcaaattta ctacatttta cacttccttc tattttgcct gttcctgata
     8281 tattaacatt atcaaaaatt ccacctttac attttccaac accatcaaat cttagtgaac
     8341 ttaattcttc attcataatg atatcctttc tttaccgtat tttatcttta aacctaatat
     8401 aacaaaacat tgttacgttg tcaagtatga taataatact taataaagca actatcattt
     8461 attttctata aatgggcata aaaataatag ggaaatgctt tatttaaact agaaatctaa
     8521 tttcttagat tgtggtttaa ataaaatata tccctattac tcaatgttta tatattcatt
     8581 gtataaactt ctaaaatata agtatttaaa ttctactgtc aatacttttt atacactgtt
     8641 tgtgctatca tttcacacct aaaggagtag gctttgcgca ctattctgta acctacaaga
     8701 cactatcttt ttctatctat tgataaattt ttctattaca tcagctagtc tctccatatc
     8761 agttggataa acttcaccta tgctacctat tctaaatgta tctatatcag tcaatttacc
     8821 tggatagata acaaatccat tatcttttaa ataagtataa aattccataa attcaaactt
     8881 tgcattttta ggatataaga aagttgttat tacaggtgat tgagcattat cattcaccaa
     8941 agtatcaaat cctaaagatt ttaatcttga agctattgta aactgatttt ctttatatct
     9001 agcatatctt ttttcaacag aaccttcttc ttccaattct aataaagctt gataaaaagc
     9061 tcttacaaca tgtgtaggtg aagtaaatct ccatttacca ttatttttct ccatagtatc
     9121 ccattgcgca tacacatcaa gagataatga tttagctata cctttacatt tagataattc
     9181 ctctttatta gctattataa aaccaaatcc aggtactcct tgtatacatt tgttagaact
     9241 actaactaaa aaatctatat taaaatcttc aacatcaatc tcaattcccc caaaactact
     9301 catagcatca actatataaa ttttgttata ttcttttgca agtttcccta cctcttgtat
     9361 aggatttaat ctacctgtag tagtttcaca gtgaaccatt gatatatgtg ttatatcctt
     9421 gttttctttt aataaatttt ctactacatt taaatccact gcttctatat ctttaaatgt
     9481 gcagtcaaca aattctatgt taagataatt acatatatct ttcattctct ttccgtatgc
     9541 tccatttgca attacaagaa gtttaccatc ttttgaaata gtacttccta ttatagcttc
     9601 tactgaaaaa gtcccgcttc cttgcattaa tacacttgtg tatttatctg tattttgggt
     9661 tgctagagat actaatcttc ttctaacatc ttgtactagg ttattatatt caacatccca
     9721 agtacaccaa tcttttaaca tagatgctct tactgattta gtagtagata gtggtcctgg
     9781 tgtaagtaat atgtaatcat tttctggtac aaaatctgat ttaatagttt cattctctat
     9841 ttttattagt atatcttcta actcagccat agtttctatt acaaaatgtg cacctgcctc
     9901 tttaaatttc tttgaaacta tactcatctt agcttttaat tcttccttat ccatgttctc
     9961 tacttcttct tgtgtaagcc ctaactcact tgaacctttt attacagcaa cactccacat
    10021 tcctgcattt acgccttctt ttacatcact tattgtatct cctactttta ccatagaact
    10081 cataggagac acccctaaag cttcagcatt tttatagcac atccaaggat atggtcttcc
    10141 ttgagatact tctgatggtg ttacaagaaa attgggtgag tatcctttct ttgctgcatt
    10201 tggttcaact atgttcatca tctctcttgt ataacccgtt gtagaaccta tttttaatcc
    10261 attttttctc aacttttcaa tagtttcaac aacatgagga attggagtcg tatactcttc
    10321 taatgtctca aataacattg gctcaaattc agcataaagt tcatttacat catcttcagt
    10381 tggcacttta ccaaacttat ctgaccaaat attctttatt ctatccattt cacacatttc
    10441 tcttatatgg tctattttta attttcccat aggttttctt gcttcttcca ttgttacatc
    10501 tatacctctt cttttaaata tctctataaa tacattaaga ggtgcaaaac atccataatc
    10561 tactgttgtt ccagcccaat cgaatactac tgcttttatt ttttctccat atatcttttt
    10621 catatttata gcctccatag attcttttta gaatttttgt gtttttaatt gtttttactt
    10681 ttaaagtata actttcatat gttaattata catcatattt aggttaaata tatattaaat
    10741 ttctgttttt agttgtattt agttgttttt attttgtatc acattcccat aaaatcctaa
    10801 gttacaatct aaaatttact ttctaatctc aatctatgaa ctgactatat tttatgaatt
    10861 tagttgtatt gagcatagtt gtattatata aaactaaggg tttattttgc ttctatccat
    10921 gttatattta tctcatttaa cttatccttc cattcattcg gaggttctac atcacaaata
    10981 acaaaatcta attcctctaa atctgctatc ttatacatac tagcaattcc tattttagaa
    11041 ctatcacata atacaaaact ttcctttgaa ttttttatat attcttttga caacaatgcc
    11101 ttattttggt ctatactact aattccaaaa ctatcaagta taccttcact agatataaaa
    11161 gctttatcta cgtataagtt tttcatagta tctattgaca ttggacatgc tgtcctttgg
    11221 tgttttaaat ttacttttcc accaataaat ataacttctc catcaaacaa accttgattc
    11281 tcataagaaa tcaattttga tagaactgga taagagtttg taataatttt taaattgttt
    11341 acagatatta tataatctac aatctgcaaa ttggtagtac cttcatctat gataattgac
    11401 tcattgtcat ttacaaattt agctgcatat ttagcaatac tttgcttttt atctaggtta
    11461 gtattgtttc tctttatatg ttctggttca atcactccat aatttatttt tatagcacca
    11521 ccatatacct tttttaattt attttcattt tctaattctt ccaaatatct tcttattgtt
    11581 tctgatgata cttctaatct ttttactaat tctttagtgt ttaccttccc ttttgaatct
    11641 aataatttaa gtatttcttt ttttctatct atcgaattta aagacattca ttttcctcct
    11701 aaatataata tatttttctc ctataatatt acaatgatac ctaagattta tgaaatcata
    11761 tttatgtatt aaatttttta cttaattaaa gttattttca aaagactttg tattatcatt
    11821 aaaaaagtcc atcaaatatt agtatcagat ggactttgta aaattttatt ttataaatta
    11881 atatttaaag tctaactact aatttatttt cttgtctcca agaattttac taaattattt
    11941 acagtagaca tatcttttct ctctaaatca gttggttgaa gctctatccc taatctattt
    12001 tcaatttcaa gtaaaacttc aattatagct aatgagtcta aaagttccgt ctcaaataaa
    12061 ttaaggtcta aatcttctct tatctcatca gtccctaata catcttcaaa tatttctata
    12121 actgtttctt gcataaattt tcctccttaa aattaagcta aataacctga gaatatcagc
    12181 aatccaaaac acacaatatg gaatgtaatg aaaatctgca ctctctcgaa ccatttatct
    12241 tttttatgtt ttttataaaa tttagatttt cgctgatata tatcagttaa cacaagtgcc
    12301 aatccttggt atacaccata agcaacataa taccaagtta gaccatgcca aaatcccatt
    12361 gttatcatgg ttatcatctg agccacatga gcagcagtag tccttttctt aaatcttttc
    12421 tttctcattg atgacataac aaatctagaa aaaatataat ctccaaacca tcttgaaaga
    12481 cttatatgcc atcttgtcca gaactctttc atatctttac tgataaatgg cttgtcaaag
    12541 tttataggta cttgtattcc aaatatgtat cctgtaccta tagcaaataa actatatcct
    12601 gcaaaatcaa agaacaggta taaactatat gcgtacatat aacttaaaat atttataaaa
    12661 ctcatatctt taggtattct tgatacccaa tatgtattta tcaaaaatgc tataacaaac
    12721 ttataaccta ctcccatgac aatatttttt attcctggca gtaagtattc ttctatatac
    12781 tcttttctag agatttgttt ttccaagtcc tgttcaaatc ttcttgacct atcaataggt
    12841 ccagaactta aagttggaaa aaataacata aaataaagca tctttgaaat tttaacttct
    12901 tttatagctc catcataaat ttcaataacc atttgtatag ttctgaaatt taagtatgat
    12961 attccaataa atccaattat tccaaaagaa gtaactggag atattttatt tattattatt
    13021 gggagcattg atgcaaacaa gaatccccaa tatatgtatt tatttttagt ttttcttctt
    13081 acatattcat atcctttaac tattattact tcaagaataa taaatattac caaatacttt
    13141 agttggacat caattcctac tattaagaat atcataaaca aacttgccaa cataccataa
    13201 tactttatat tcttgcccat tagacctaaa attactgctg gtattgatgt taataataat
    13261 atataaagat aaaaataatc tccatactgt gaaaaaatca ttaaatttcc tccataagtt
    13321 tctttctgtc tattttacca ttgatatttg ttggaaactc agagattatt gaaatatttc
    13381 ttggaatcat atatgaaggt atatactttc caagttcctt ttttattatc atcccatttt
    13441 taatattact taaatcattc ttttcattta actcaacaat accttttaag taagcaattt
    13501 tttcgtcctt atatactggt aaaactactg cattttttac attatgtact tttctcaaat
    13561 tattttctat atcttctatt tcaattctaa atccatttaa tttaatctga aaatcttttc
    13621 ttccacaata atatatattt ccatctaaaa gatatcccat atctccagtt ttataagctc
    13681 tccactttac accatctatt tcatcataga aaaatacttc atcagtcttt tctttattat
    13741 tgaagtaacc tttagaaact gatggtccaa ttattataat ttcacccttt tcattttctt
    13801 taagctcatt tccatcttca tcaagtattt ttattttaca atttgacata gggtatccaa
    13861 ctggaagact tttttcatca tctatagcct tttgagtcat atcatttaca cttacaccaa
    13921 cagtagcctc ggttggtcca tatccattga taattcttgt gtttggaaat ctactcataa
    13981 gttctttagt aagatttttt gaaagtgctt ctcctatgaa tatcattgat tcaaggttag
    14041 gaagcatttt actattaaac tctttttcag ttatacatac tcctgcaaaa gaaggagttg
    14101 acacccatac agctatatct gagatactaa attgtctaaa tagttcttta tagtctgcta
    14161 atacatcttt tgatattgaa aataatgtag ctccatggat aagaccaggg tatattgttg
    14221 taactgaaag gtcaaaagaa tatgctggtt ggttcattat tactttttca cttccatcta
    14281 tgtttagata tgggcttatc caatctgaaa agctatctaa attatttgaa cttatttgaa
    14341 ccccttttgg ctttcctgta cttcctgaag taaatagtat atatgcattt tcatcatctt
    14401 ttacccagtt ttctttatct aatgattttc cctgatactc atttattatg tcatttaatt
    14461 tatccatatc agttacattg atatctccaa aatctttttc atcactaaag ttaaataata
    14521 cctttggttt tatctcttta gtgacttcaa aaactctatc aattggaaaa ctaatatcaa
    14581 gaggcacata cgctctacct gattttaaag ctcctatcat acatgccatt atcatgtttt
    14641 ctttatttcc ataaattact ataggagtat cttcttcttt gtatacatcc tttagaaata
    14701 ctgatatagc atctgaatat tcatttaaat ctttataaga aagtttatct ccattacaca
    14761 ttagtgctgt tctatctgta tttgaatatt tttttattcc ttcaataatt ttcatataag
    14821 aatgctcctt taaaatccgt tataaataaa tggcaattct ttaaatggag taaatgttag
    14881 aactcctatc gccaatatta taaccatagt taaacaaata ctagttaaca aatctttatt
    14941 gttctttaaa tattttaaat aatctctcac agacatctac ccatcctttc gtaccaagat
    15001 gcattacatc ccttaaataa tacttatctg accctttatc tttaaggttt agaacttcaa
    15061 agcctttgct ttctgctatt ttttgtgctt tatcgtagta ctgatttctt tctttttcag
    15121 aaataccagt caaattatag aatttatcca tacttgggat taatacaaca actggcttaa
    15181 tacctaagtc agtacataca tttagcatca atttataatt ttcaaactcc ttagatgtta
    15241 ataaatttac atctttgtct cttcctttgt attgctctat accatcttta aaatgctctt
    15301 tataatagta cttatcaata tttagtggat ttttaccaac tcttttttta gcatcttcaa
    15361 tggctttctt tctctcatat gaccaattta ttggtctttt tctttttgtt gctctctttt
    15421 tatctagctt aattaatcgt ttataaagaa tacccttttc tttaagagct atacaatact
    15481 ttcttccttg aaaataaggc tctaaaagca ccttttcagc tttttcagga aatgtcttag
    15541 gctcatatag cttagcatat aatgcttctg ctttatactc gtcacttccc cataataact
    15601 tacttgattt ttttgcatat tcaatcttat tttgtttaga tatttttgga ttatctaaaa
    15661 atcgataaaa ttgtattggt gaaaatctag actgataatg gtgacttgtg actccgtctt
    15721 tttccataaa ccattgcata gaaattaaca agacaacttt cttattatca atattaggat
    15781 tcatacttcc taatattgca gcatcttgta atgtctgagt ataggctctc ccaattgcaa
    15841 acaccttatt tttactccta tttgtattaa aatagtactt tggatgttgt tttgttgaat
    15901 tactaagttc tgatgacccc aaaatcataa tatccttttc tcttaaaaaa tgattatttg
    15961 ctgtaacccc tttatctttt atatcactta aagtatcatc cataattgga agtagatttt
    16021 tctccctcaa aagttcatca gttttagaat caagaaattt gtctaaccca aataaaaata
    16081 ctacgcctat tataaaaggt gtaataaagt atatcaattt tctcatattt tctctcctct
    16141 aaaaatattc aaatttatgt tattattaat catattagtt ttgatatgta tattcaacta
    16201 caaaattgta ataaaatcgt caaaactttt tttgatactg ttacaaaatt gtaaaaaaaa
    16261 agaaaaaaac gccaaaaata aatttctttt tagcactttt tcatacttat aatttgacat
    16321 tttaacactt ggttaagtat acaccttacc ttaacttaaa attaaattta tttaagaata
    16381 tatattataa attcttatat tttctaatat aaactatata ctatcagctg gataaacaat
    16441 actactttgt cttcttatct catccataat ttccattact attattgaat tttcatgaga
    16501 atttatattt gattctattt tattgttatt tataagactt ataaactcta caagttcata
    16561 atacattcct tctatttctc taggcttttc ctctgctttt tcttttggaa tagatatatc
    16621 ttcttctctt ccatctctgt atttaataat agctttttca aatagattaa gcttttcaat
    16681 tattatactt ccctcttctc cttgtatttc agatggtata taagaatcag ctactttaga
    16741 atactgaatt atcgcatcca tatcatcata ttgaaataca gctgaacctt ctccatccac
    16801 tccagtttga agtatatgag aaactgcctt tacagttttt ggagcaccaa ataaattcac
    16861 cataggatgt atacaataca ctcctatatc cattaaagct ccatttgaca actctttttt
    16921 aaaggcattt tctataattc catttttata tttatcatat cttgaagaat actgacaata
    16981 gcttccaaaa aatcttctta ttttccctat tttatataag ttttctttaa ctgctttaaa
    17041 gtttggaaca catgtaattc tcatagcttc cataagcaat acattattat ctttggctgt
    17101 ttttatcatt gatttgactt cttttaaatt agatgctaac gacttttcac aaagcacatg
    17161 ttttttgtgt tttaaacaag taatggtttg ctttgaatgt aatgcatttg gagaagctat
    17221 gtatacagca tctatatcct tcgatttagc catttgttct aaatcatcaa aaaaaatact
    17281 tgctccatat agagttccaa attctctagc tttttctaaa tttctagaat atacagcaac
    17341 caattcaaag tctttaaccc tagatgcagc tcttagaaat atatttgcta tattgcttgt
    17401 cccaacaata ccaaatctta ttttacccat cataaatcac tctccatttt ctacacttta
    17461 ttttaatttt aataaaaaac tttacaactt atagatatat tataaaataa ttttatcaaa
    17521 taaaaaagaa agctaatttt aaatgatttc accattttaa catagctttc ttttaactac
    17581 aatctaaatt caacttatat ttttaaagtt atctttctac tatatcttta tttcccctca
    17641 aaaattttat agtaagaatt tcatccacaa acaatgttac taaaagtata gttacaacaa
    17701 ttggccaata aggtattata agactatata aactatcaac tatatacact tgaaaatatc
    17761 taactccaat aaatgtcaat atagtccaat atacagaaaa ctttagacat actattcctc
    17821 tactattgta cttataatct gaataatccc aatagacctt attaaaatat actttcataa
    17881 gtattcctgt aatatattct actactgtcg gcactactag acataataaa agcaatgcaa
    17941 atatattatt ggggtcaata aatcttgaga cctctattat aatcgacatt gcaattgcat
    18001 acattggttt aaatggacca tttaaaaatc catcttcttg aaaatgccct tgtttataat
    18061 aactatataa gttttcaatt atccaaccta taaaaccata caagaaaaaa ttaaataaaa
    18121 aatataatag agaacctaca ttatccatga atattcctcc tagctgttta tacttatttt
    18181 ttctcatatt catggattta ttctaaatca aagaaattat atttaattta tttaattaat
    18241 actttatatt atagattgtg acattcttta gagtttaaac tttttaggta ttctctaact
    18301 tccagtaaat tttcaagtat cttatgtgat tgtgatttaa tttgctcatc tggctctttt
    18361 aaatctggta catttataca tcttatacca ccattgtatg cagcttcaac tcccatagga
    18421 gaatcctcta tgacaataca atttttggga ttaacattta tctttttagc tgcttttaag
    18481 aatatctcag ggtttggttt agagtttaca acatcatctc cacaaactat agcatcaaag
    18541 taatctttta aatttgcctt agctaatctc tttactgctc tttctctctt tgttgatgtt
    18601 gcaacagcca ttttgtatcc attttccttt aaaaaagaga ttagttcatt gactcctaat
    18661 ttgataggag ctcccttcct ctccataaat tcaatcatgt ttttagtttt ttcgtcatat
    18721 aaatctatta ttggtacaga actatcatat atatctgtaa gaccttctat tattccttta
    18781 cgatttcttc ccataactga tgtataaatt tccttagtca tagtatatcc atacttttca
    18841 aacgtctcca tccaaaattc aagagaaatt ctctctgagt caaacaaaac tccatccatg
    18901 tcaaaaataa ttccttctac tttttgcata caattcctcc aatatttttt gatataatta
    18961 tattacactt tttatgatta attttacaac cctcaattta tccaattcta atattgtaaa
    19021 attgtaattt tcaaaagtta cactttggtt tactttaata tttgaaccaa gcatatagga
    19081 tatccaccca cctatactgt caaatccatc atgctctata tcaagtccaa aatacttatt
    19141 aatatagttg atagattcag ttccatcaac taagtaagaa cctgctccta ttttcttaat
    19201 atgagtctct tctttatcaa attcatcttg aatttctcca actatctctt ctagtatatc
    19261 ttctattgtt accacaccag atgtaccacc atattcatcc acaactatag caagttgaag
    19321 tttttccttt cttattctct caagagcttt atctatagtt aaattttcag atatatatat
    19381 aatatctctc aatatttctt ctagttcaat tttattttca tttatctttt gattgtataa
    19441 atctcttata tgtacaaaac ctaatatatc atctttattt tttctgcata ctggatatct
    19501 tgtaacacct tcctctttta aaatagctaa tatcttttct tcagaatcac tttcatatat
    19561 acataccata tcagttctag gaaccattat ctctctaatt ttcttttctt caaattcaaa
    19621 aatattatca actaatcttt gctctgattc atctataagt ccacttttat aactttcttc
    19681 tactaatagt cttatttcgt cacctgtatg gggttcatca acctcatcag cttgtgaata
    19741 tccaaatggt ttaagaaata aatcagtact taagttgaaa aaatatatta ttggatatgt
    19801 taacttataa aatcctacta ataaaaaaga tgtactcaac attatttttt ctgtattata
    19861 aagtgctaaa gctttaggaa ccaactctcc tattacaacc tccatcattg ttataattaa
    19921 aaaagaaatg gaaattgata tagtatatat aatactttca ctaagattaa atacacttac
    19981 tattggtaaa attaactctt taactgtaga ttctcccatc caacctaatg caagtgaaca
    20041 aagtgttata ccaagttgac atgcagaaag ataggaattt aagttgtctt ttattatttt
    20101 acatcttttt gcattattat taccttcagt tatcatggta tcaatcctag aactacgaag
    20161 ttttaccata gcaaattcac aagctacaaa aaaagcattt atcaaaacaa gcattactat
    20221 aagtgttact tttaataact cccgcataaa gtattggttc ttataatgta agaaatctat
    20281 aagaactcaa agttcccccc ttatatttat aaatttaatt tataatatat attctatcat
    20341 atttattaat atatcctact ttattacaaa taatttttat aaactaaata acccctttta
    20401 atatttatga ctggctattt gataaatatt aaaggggtta tttagttatc attttttatt
    20461 tctaaaaatc ttttctcaaa tga
//
LOCUS       DAEMHW010000057        20105 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEMHW010000057 DAEMHW010000000
VERSION     DAEMHW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.057"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(889..3996)
                     /locus_tag="KPK67_001747"
     CDS             complement(889..3996)
                     /locus_tag="KPK67_001747"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBG2458733.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCDEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(4089..4321)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4496..5023)
                     /locus_tag="KPK67_001748"
     CDS             complement(4496..5023)
                     /locus_tag="KPK67_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBG2458734.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(5058..5843)
                     /locus_tag="KPK67_001749"
     CDS             complement(5058..5843)
                     /locus_tag="KPK67_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBG2458735.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIINLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(5853..6110)
                     /locus_tag="KPK67_001750"
     CDS             complement(5853..6110)
                     /locus_tag="KPK67_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBG2458736.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(6129..6587)
                     /locus_tag="KPK67_001751"
     CDS             complement(6129..6587)
                     /locus_tag="KPK67_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBG2458737.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(6627..7331)
                     /locus_tag="KPK67_001752"
     CDS             complement(6627..7331)
                     /locus_tag="KPK67_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBG2458738.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(7341..7976)
                     /locus_tag="KPK67_001753"
     CDS             complement(7341..7976)
                     /locus_tag="KPK67_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458739.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            8357..9397
                     /locus_tag="KPK67_001754"
     CDS             8357..9397
                     /locus_tag="KPK67_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2458740.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(9860..11215)
                     /locus_tag="KPK67_001755"
     CDS             complement(9860..11215)
                     /locus_tag="KPK67_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBG2458741.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(11241..11510)
                     /locus_tag="KPK67_001756"
     CDS             complement(11241..11510)
                     /locus_tag="KPK67_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG2458742.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(11799..13739)
                     /locus_tag="KPK67_001757"
     CDS             complement(11799..13739)
                     /locus_tag="KPK67_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458743.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PSLNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNSDEKQPNTPKDD
                     VESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVFGTNEH
                     SFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCILTPIMI
                     GSILIVNPDTRKSIIKFIKLKK"
     gene            complement(13922..15397)
                     /gene="spoIVA"
                     /locus_tag="KPK67_001758"
     CDS             complement(13922..15397)
                     /gene="spoIVA"
                     /locus_tag="KPK67_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBG2458744.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(15569..16864)
                     /locus_tag="KPK67_001759"
     CDS             complement(15569..16864)
                     /locus_tag="KPK67_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2458745.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYNTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENATKVS"
     gene            complement(17048..18457)
                     /locus_tag="KPK67_001760"
     CDS             complement(17048..18457)
                     /locus_tag="KPK67_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2458746.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(18509..19648)
                     /locus_tag="KPK67_001761"
     CDS             complement(18509..19648)
                     /locus_tag="KPK67_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2458747.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            19967..>20105
                     /locus_tag="KPK67_001762"
     CDS             19967..>20105
                     /locus_tag="KPK67_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2458748.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     ST"
BASE COUNT         6225 a         3647 c         2229 g         8004 t
ORIGIN      
        1 acctatcata atattaacaa atgtaattat ttaaaacaat tatttagaag gttttatatt
       61 taattttaaa taacatattt aagaaccgta agtcttaact tttatcattt ttgaggttgt
      121 cgttcacata agttcgctac tacctatgca gttctcttat gaacttctta cactttcacg
      181 taagcacaga ctatatctta tccttcggca ttatccgtta aggtctaccc acttccacta
      241 ccaatagctt gtagtgtact tccctcaaga ggaatagtcg ttgaagtttc tcctattcgg
      301 agtttacctg ctgattgccc attttaaagt acttagggtt taaccttata ccatctaacc
      361 aattttttct actttcgtaa ctttcacact taggtttatt tcatccttat gttttagttt
      421 ggttagcttt aggggtttcc agcaattcaa gtagtattgg ataggttcta tcctatctct
      481 acatataggt ttccctatat gctgactatt ttagctacta actcatgact aaagtcacga
      541 gtgtgcgtag ctatttttta atcaatactt aactttttgt tttcaccata catcaaaacg
      601 cacccctttg cataataaat aaaccaccct gcataacaca cgataaattt acaaataaat
      661 ttatcagaag tagtttccta aaaatattat tcatcctcac ttaaacgttt attcattaat
      721 attctatcac atgacataaa taatttatat gatttatata gatttattta ttgtaaattt
      781 tggaatatta aaacatttta ttgaatagat aattatatta cataaaaaat ccctatagaa
      841 atctataaac taatttctat agggattttg gttaactaac tattcttttt atttttttat
      901 aacttttata cctgtcatat ggtcatttaa gttttgttcc tctaaagatt tatcatctac
      961 tctgtttatt tctaaagcta aagtttcaga cttgatatac tcattaaaat ggtcaacagc
     1021 cttagcaatt tcatcatcac cacagtattc tattactata ttatctaata cttcaaagtt
     1081 attagatttt cttaattgct gtactttaga tataaattct ctagcataac cttcattaat
     1141 caactcttca gttagcttag tatctagtat aacaaaaaga ttgttttcaa cagatacatt
     1201 aaatccttcc ttagcagtta tacctattaa tactaaatcc ttattaaatt caaaagcttc
     1261 accatctaaa tcaactgtta aagtctcacc tttttcaagt ttattagcag tttcatgagc
     1321 atttaattta cttaatgctc caacgaataa attcatctta gaacctaata atggccctgc
     1381 ttccttaaag tttggtttta aactaaagtt catatactca tctaaatcct tagcaaatat
     1441 tacctcttta acattaagct cttcttttat taaatcaact acatcactta ttgtatcttc
     1501 aaacttacca tctactaaca ctttttgtaa tggttgacga acttttattc tttctacttc
     1561 tcttgaagct ctacctaaag taactaagtt tttagctaaa tccatttttt cttctaattt
     1621 agtatctact aactcttcat cacatttagg atattcagct aaatgaacag atacttctcc
     1681 tgttaagttt ctatacattt cttctgacat atatggtgca aatggagcta tagctctaca
     1741 aagaccatgt aacaattcat aagttgtatt gtacactgct tttttatctt ctgttaattc
     1801 agttgcccag aatcttcttc ttgaacgtct tatataccag ttagataaat catcatttat
     1861 aaaatcttgt atcattctta aagttttatt taactcaaat atagctaaat tttcttctac
     1921 atctttcatt aaattattga acttagataa tatccatcta tcaagttctg gtctatcttt
     1981 atattctaca aaaaattcag ttggatttat atcatctgtg tttgcatata aagtaaagaa
     2041 gttatataca ttcttcatag tacctataaa tttactttgg atttctttta atccatccat
     2101 atcaaattta gttggagtcc atggtggaga aacatatagt aaataccatc ttaatgcatc
     2161 tgctccatat tggtcaaata attccattgg atttactgta tttcctcttg atttactcat
     2221 tttcttacca tctttatcaa gtacaaggtc tggaactaaa acactcttat atggtgcttt
     2281 tcctaataca aatgttgata ctgcaagtaa tgagtagaac caacctctag tctggtctat
     2341 accttctgat atatagtcag ctgggaacaa ttggtcaaag ttttccttat tttcaaatgg
     2401 atagtggtgt tgagcaaatg gcatagcccc actatcaaac caacagtcta taacctctgt
     2461 tactcttgtc attggttttc cacatttttc acattttaag tgtatatcat ctacatatgg
     2521 tctatgaagt tcaactgttt ctggatttac atcttctata gctttttcag ctaattcagc
     2581 tcttgaacca actgaatctg tatgaccaca ttcacatctc catataggaa gtggagttcc
     2641 ccaatatcta gttcttgata ttgcccagtc atttagattt tctaaccagt ttccaaatct
     2701 cttctctcct acaaaacttg ggaaccattt tacaccatta ttattttcta ttaatttatc
     2761 tttaagttta gtcatctcta tataccagct tggttttgca tagtatacta atggagtttg
     2821 acatctccag cagtgtggat aattatgaac tactttttct ttgctatata atttattttc
     2881 agaagctagc cattttatta tttcaacatc tagcccatct tccattacaa atctaccttc
     2941 ccaaggagta gatgtaaatt taccagtttc atctactggt tgaataactg gtaaatcata
     3001 tcttttacct gtgttgtagt catcttcacc aaatgctggt gctgtatgaa ctataccagt
     3061 tccatcttca gtagttacat aatctcctac tgtaataaaa aatgcttttt tgtcaacttc
     3121 tacaaaaggc attaattgtt catattctaa accttctagg tctttacctt tcatttcttc
     3181 taagacttca taatcttcac ctagtacttt atcagctaaa ggtttagata cataatatac
     3241 ctcatcacct tttttaactt ttaaataatc tacatcagca tttactgtta atgaaacatt
     3301 tgatggtaaa gtccatggag ttgttgtcca tgctaagaag tattcatcag catctttttt
     3361 cttaaattta gctataactg tattagtttt tacttcttta tatccttgtg caacctcatg
     3421 actagcaaga ccagttccac atcttgggca ataaggaagt attttatgcc cttcatatat
     3481 atatccttct ttgttgaatt tgtttagtat ccaccataca gactctatat agttattatc
     3541 taaagttata tatggattat ctaaatcaac ttcataagcc attctctcag tcattattct
     3601 ccattgtttt tcaaatgaga aaactgactc tctacatttt tggttaaatt cagctattcc
     3661 atattcttct atttgttgct tactagtaag tcctaactct ttctctactt gtatttcaac
     3721 tggtaatcca tgtgtatccc aaccagcttt tctctttact tgataaccag acattgtttt
     3781 atatctacaa actgaatctt taagcgttct agataaaaca tggtgtactc caggatttcc
     3841 attagctgtt ggaggtcctt catagaatac gaaacttggg tcatctttcc ctttttctaa
     3901 agttttttca agtatattta tgtctttcca ataatcaaaa acttgtgctt ctgcttgttt
     3961 tacagaactg tctaccaatg gcttaaattt cgccatgtaa atcacccttt cctactaaat
     4021 attataattc caaaattttt tattctttca cataaattta aatactaaaa aactcgcccc
     4081 ctttaaacca aagggacgag ttataaatcg cgttaccacc ctaattctat atgtaaacta
     4141 aatactagct acatataaca ctctaatagt ataacggtac taaccggcac agcttagtag
     4201 aatttaatcg ttcagcctgc agctcaagag tgatttttat ctttataaat tcattggttc
     4261 tcagcatacc caattctctg taaaattatc taaagataaa tgtctctatc aacgctttta
     4321 aaaatttatc tgtgtttata tgaaattcta taacaaataa tttgaatagt caatatattt
     4381 ttgaaaaata taataatttc tatttttctt acatttttat cctattttta atctttgtta
     4441 tagatttctc aaactcacac aattattcta cataaaaaat aaacaaatta caagcttatt
     4501 ctaaagttgt agcagcttca tcattactat aattatacac tgttgttcct tcaaatgcgt
     4561 ttacacattt ttcatctaca tcataaaaaa tctcatcaac acttcttatt tcatcttcta
     4621 acagtgattt aaatttattt ctaaatatct taaattcttt gactaaagca tcatactctt
     4681 ttcttatctc tataacacgg ttattagctt gttctataat ttgtctagct tttaaatcag
     4741 cttcttcaac aattatctta gcctttttat tagccgcgct acaagtgtct tctgctgcac
     4801 tttgagcagt tattaaagta gcttttagtg tttcttcaat attttcatac ttgtttactt
     4861 ggtcttgata taaccctaat ttttctttaa gagctgtatt ttccctacat agagattcat
     4921 aatcttcctt gatgatatct aaaaattcat caacctcttc atctctatac cctcttaaac
     4981 cttttttaaa ctctttattt tctatctcaa ttggagttag cattacattt cctcccagct
     5041 ataatttaaa ttagtcctta tacaattaat cttgcttgaa cttttatttt tcctttttta
     5101 gtaatatctc ctatttgtgt taccttagct cttcctttgc ctctaataga aatcaaatca
     5161 tcatttttta ctattttaga agttgaagtt atcttttcat aattgacatg gactttttct
     5221 ccatttatgt attttgcact atcttgtcta gatatattgt aaataccact tatcacacaa
     5281 tctaacctat ctgaagatac tgtaaatgat acttctttat attttgaaga actattaaca
     5341 atattttctc tggaaatttc ctttacaatt acattatttc ttgaaacctt ttctaagttt
     5401 ataattataa aatcgcaaat atcactactt acaactactt gacaaaagct atcatgtata
     5461 attatatctc caattttttc tctttttata cctagaccca gtattgaccc taagtaatct
     5521 ttatgagata tatttttaaa tttgaaattc ccttctattt gtaaaaatct aagagtatct
     5581 tctatatctt catattccat gtaaaatgga taaataaata cagtgcttct ttcagcttgt
     5641 tcataacctc catccacact gtattttatt tcatttgtag aatttaatat tgctacagca
     5701 tttttcactt catatggatt aagaaactcc gtagacttaa catcatagtt ttttatacaa
     5761 ctattagcct tgtctatcac tttaaacatt ttatttttca agtctatatc ctttatgtga
     5821 tttgttagtt ttaatttatc catcctatca ccttagaata taaccaatac taattgtctt
     5881 aggaacatca ataatactat tgcaattaaa ggagaaaaat ctataggtcc atttgtatat
     5941 ttatacatta tagaccttat tggcccttct attggctctg taagattttc caaaatatca
     6001 aaaattttac tttgtcttcc atttggaaac caagtcatca agctttttat aactatcatc
     6061 catgctatta tatctaaaag ataataaagt gcaattctta ttgttcccat atattaaccg
     6121 cctttttact atttattttg ccaagggaag aatgctttac tttctaattc ttttttcata
     6181 tttgcatcta tatccacatt attaggagct agtataaata ttgatttaga aaccttttgt
     6241 atacttccat ctaagacata aactgcccca ttcataaagt taaatataga tttacgaact
     6301 tcagaattag tcaatccttc caaattcact ataactgccc ttctttgttt tagatggtct
     6361 gctatagttg cagcttcatc ataaacctta ggttcaacta ttacaacttt catttggcta
     6421 gttgtatgta aatttacaat tttatttgct tttgcagtac taaagccact gttcatttct
     6481 tcttcttgaa ctatatcgtc catgccactt tcatattcat cttcaatgta atcttcttct
     6541 tcatcaacta tccaattctt aaatttagat attattccat ttgacattat taacttcctc
     6601 cttaaaattt attcaaattt gagtaattat tttttattgt agtttctttc tccaaagatt
     6661 gatgttccaa ctctaacaat ggtagcccct tcctctatag caatttcata atcgtgactc
     6721 attcccattg acaaaacatc catccctaca ttagggatgt ttagttcttt tatttgtttt
     6781 gataaatctt taagaccttt aaatacacct cttatctcat cttcatcttc tataaaaggt
     6841 gccattgtca ttagaccctt tatttttata tttttatatt tttctgacac ttctttgaca
     6901 aagtctacaa cttcttctct atcaagtcca tgcttagact cttctttaga tatattcact
     6961 tgtactaagc aatctatttc tctgtttatt tgttctgcac gtttttgaat ttcttcgcat
     7021 aatgcaattc tatctaaaga gtgtatcata caaactttat cgattatgta cttaacttta
     7081 ttggtttgca aagttcctat caggtgccag tttaatttgt cacctataat atcatacttt
     7141 ctagctaatt cttggggttt attttcacca acagaagtga ttccttccct cattgcctca
     7201 ttaactaagt ctacatcaac agtctttgtg actgccaaaa gggtaatctc tttgctgtct
     7261 ctatctactt tttttgcagc tttatcaatt ttgtttctta tctcagacaa attatcttta
     7321 atagaaccca ttattatcac ctactttatc cttatatttg tgtttatact gttaggtctt
     7381 aaaattattt tgtcatatag attaacactt gtaactccat ttatgtcatt tttataataa
     7441 tccacaaata cagaagattc attctcataa gttattcctt ctaatatgac aaattttaca
     7501 ctttgtgatt gttcatcaat tacatataca ccacgtttat tgtcttttgt ttttatagaa
     7561 gttttaggta tttttaaaca ttccatctga ttatatatta tatcaaattc ttcgacactt
     7621 gtatcctgat ttgcaacaat ttggttactt atttttaatt ttaccattat tttattatta
     7681 ttttcatata ttttgtaaac ttttgcatct aatttttgct catgatattc aattattaca
     7741 ttttgatttt tttcaaagtt attagaatct tctttactga tgtatgtaga tagataatta
     7801 ctctggtcat tggcatcttt ttctatagtt ccttttacat gaatctttgc atcaactttt
     7861 tcatttttta acaccaacgt atctacattt ttgctaatta tcattactaa tatattaaat
     7921 actaaatata taagaatacc aagtatcact aatctggtat attttacctt tttcaaagac
     7981 tctcccccta taaaattact ttcattataa gtacttcttc taaaaaatgt ttattccttt
     8041 tttataagct aatattttac gttctaataa ttattttttc catattaaat ttatttttta
     8101 tcaatcttcc tatacaagac tgcattattt atatataatt gtattataaa gtataagttt
     8161 aatttacatt ttatacgtta agcttacttt atatgttttt atagttattt ttatttttag
     8221 atacaatata aaaccaatta agatttaaaa taacttttgt tatgttgaca tttatttagg
     8281 taatcattta tgaattagga taacgttatc ataacaaaag tttatgtcct aaaaaaaata
     8341 caaggaggat tttcttatgt cagaaatttt attaggaaca ggtttacttt tattggttct
     8401 atgttgtttc acaattttta gttacaaggc cccatatggt atgaaggcta tgggagctct
     8461 agcaaacgct gcatgtgcta ctttcttagt agaggcattc aacctttctt tacttggtga
     8521 tgttttaggt atggaatttt ttagaagtgt tggagctgct aatggtagtc ttggtggagt
     8581 tgctgctgct atacttgtgc ctttagcctt aggtgtatct ccagtttatg cagttttaat
     8641 aggattatct tgtttagatt tttctatatt atctggattc gttgctggat actttgtttc
     8701 tttcttaatt aagtttatag agaaaaaagt gccacctgga gtggatttag tggttgtaat
     8761 tcttctatgt gcaccactat ctcgtcttat agcatcaaat gtagacccat tagttaatag
     8821 tacactacta caaattggac aagttttgat agaagctacc aacattagcc ctataataat
     8881 gggaattata ctaggaggtc taataacagt agttggaacg tctccactta gttcaatggc
     8941 tcttacatct ataattggtc ttactggtct tcctatggca ataggagcat taggtgcttt
     9001 tggttcagct tttatgaact caacactatt tgcaaaattg aaattaggtt ctaaaaaaga
     9061 tattatatct gttgctattg aaccattaac ccaatcagac ataatttcag ctaatccaat
     9121 acctatatat tctactaatt tctttggagg agccttttct ggtatcattg tagccttgat
     9181 gggtcttgta aacatgacac caggtacagc tacacctgtg gctggttttg ctataatgtt
     9241 tgcttataat ccagttcaac aagttcttat aacagctggt ttatgtgctt tattcaatgt
     9301 aattgcagga tttttaggtt caaaaatatt cagtaccttt aaagttctta ctgctggtga
     9361 aatacgtggt actgatgtag aagaagaaat tgcttaaaaa acaataagat ttataaattt
     9421 atatctcaag tagtagagtt ttatattgta ggcaatttta ttaattgcaa acgttttatt
     9481 ttttatttat gtttatgatt attatgtgtg ttgaatattt agataaattt ttatttatta
     9541 tttttattta gatttttgtt tagtttttta tttggttgat atattaggaa taaaaattag
     9601 tatagtttat tactagttag atagcataac tttttagata cataaaaaat tttatattaa
     9661 ttagatatta ttaaaataaa agcgatacat gaatttttaa atgtccatgt atcgctttat
     9721 ttttgccata atcttaacaa ctgattttta aagtttatta tcttaacaaa gttcattatc
     9781 ttatataata tttaaatgca taggcttctt taatttaacc atctaaatat ttaattaaat
     9841 attattatat gtaatataac taatttttaa atgaatgtat aggagctgga atccttcctc
     9901 ctctttgagt aaataattca gatgaatttg tatttatagg cattactggt gctcgtccta
     9961 aaagtcctcc aaattctacc atatcaccta catcacatcc tggggctggt ataattctaa
    10021 cggctgtagt cttattatta atcataccta ttgctgcctc atcagctatc atggctccaa
    10081 gcgtacctgc tgtagtattt cctggcacag ctatcatatc aagacctaca gaacaaatag
    10141 cagtcattgc ttcaagctta tctatactta aagcaccttt aataacagca tcaatcatac
    10201 ctgcatcttc agatactggt ataaacgctc cactaagtcc tccaacatgt gaacatgcca
    10261 ttactccacc ttttttaaca gcatcattta aaagtgctaa tgctgctgta gtcccatgag
    10321 taccaacaac ttcaagaccc atttcctcta atatatttgc taccgaatca ccaatagcag
    10381 gtgtaggagc tagtgataag tctattatcc caaatggaac atccaatcta ctagaagctt
    10441 cctttgccac aagttgtcca actcttgtaa ttttaaatgc agtcttttta actgtttctg
    10501 aaacaacatc aaaaggctct ccctttactt tctctaaagc tctcttaaca acaccagggc
    10561 cacttacacc aacatttata attctatcag cctcaccaac accatggaaa gctcctgcca
    10621 taaatggatt atcttctact gcattacaaa atactactaa ttttgcacaa ccaaaacctt
    10681 ttgcatcttt tgttaattct gcagtttctt ttatgatttc tcccatttct ttcactgcat
    10741 ccatatttat accacatctt gtagaaccta catttactga agcacatact ttattagtac
    10801 ttgctagagc ttttggaatt gacttaatta gtatcttatc accttttgta tatccctttt
    10861 gaactaatgc tgagaatcca ccaataaaat ctatacctaa atcctctgca gccttatcca
    10921 atgtctgtgc aaactttaca tagtcatttt catctgttgc acctgcaata atggaaattg
    10981 gagttactga taccctttta tttacaattg ggataccaaa ttcacgctct atatcttttc
    11041 caaccttcac caaatcttta gcagaactca taatcttatt gtatattttt tctctagctt
    11101 tatctccatc agggtctata caatctaata aagaaatccc catagttata gttcttatat
    11161 ccaactgttc ttcatctatc atctttattg tttcaagtat atttcctaca ttcactatta
    11221 tcacctcccc ttactgtcta ctacagatta tgcattgaat taaatatttc ttctcgctga
    11281 atttttacat ccactgataa tttcttacct ctttcaacca agtcctcaac cgttttctca
    11341 aaagaattta atgatttctc caaatcaact atcataatca tagtaaaata atcctgtaat
    11401 attttttgtg atatatctac tatgttgata ttttgcttgt ataattcatc actaattcct
    11461 gccactatac ctggtttatc ctttccaatt actgttaaaa ttgctttcat aataatatcc
    11521 tcccttattt ttttataaat cttatatttt tacaaattga actatgtgtt aacatatatt
    11581 tcatttattt gttagtaaac cactttttat gtatatattt aatatatttt aatacctata
    11641 cattcatttg tcaatttttt tcaatattct attattttta tactatttag aaaattcata
    11701 tattatatat gttttctcaa caataaatac agttttcata ataatgaaag gtattactta
    11761 gcaatctcat gattttctaa ataatacctt tctaaaaatt atttttttaa ttttatgaat
    11821 ttaattatac tttttctagt atcaggattt acaattaata tagaaccaat cataattggt
    11881 gttagtatac aaaataaaaa aataatatct ttttttagat tatattcttt agattctaat
    11941 ttataattat tatttattat tccatcataa tcacttatat ttgacttatt tttatttgta
    12001 tcttgatttt tgttagaaat actttcccta taaggaaaag aatgttcatt ggtaccaaat
    12061 actgattttg agcttaaatc ataattatca tcattttctg taattgaatt tttattgtaa
    12121 aatccttttt ctttatcaga attattttct ttattaccgt ctttaggtat atcatgagga
    12181 tttttatttt cattgctatc agtacttgat tctacatcat ctttaggagt atttggttgt
    12241 ttctcatcag aattattatt ttcattatta ttgttttgat tgttattact atcattattt
    12301 tcatttccat tattcccatc ttgattgtta tcatcctcat ctctatccgg gtcgtctcta
    12361 cctcctcctc cacggtcacc atcaatatca tcattttccc gttcatcatc tggcgtctct
    12421 tggttgtctc cgcctcccat tataaatcct ggtttaaaaa ctatatttga atacccttct
    12481 tttaatcctc ctacaacttt tactctaaaa caacgcaact taaaatcttc aaaaaaaagt
    12541 atagcttgat ctctaatatc ctcctgctct tcagatatcc aattgattcc atcgcttgaa
    12601 tattcaacaa aaaccttact tgcactatac ggcatatcat aaaataaaac agctacatat
    12661 ccattttttg tattttgaaa ttccagttcc aaattgtcta ttggtttttt ctctataaca
    12721 gatacattca aactaggaac tacatcatca tttattataa ttttttcttt atttaacatt
    12781 tctgttttaa actttcctgt tatattaaaa ttttctccct tttccaactt ttcataataa
    12841 tcagtaaaat cccattctac atctatatca agtcgattac cactagatgt ttctaatacc
    12901 atatatttgg gaaattttat attttctatt ttatcatctt tacttacttt tatttcatca
    12961 ctaaatacac caattcccct atgacaacat ttcacaatat tgtagccaga atcttcagct
    13021 atagaaggta taagctcatt ataatcatca attatataaa ctttcttact ttctgatata
    13081 attgattgtg ccaacttatt attatctttt agtaacccac tttcattgtt gctactatat
    13141 gcttttattg aagtttcttc tatctcaaca tcacctttag aataaacacc tatagcatgt
    13201 tctccattca cttcaatatc cttatttagt attctaatat cattttcagt ataaattcct
    13261 attgcatctt tcccatttgc ctttattttt tcaacagatt cgtaggatga tttaatatgt
    13321 aagtctcctc cttcttcaac gtataaggct gtcccatcag tagcaattat gttgtttata
    13381 ccaatactta tacttccacc attttctata tgtattactc cttcttttcc tccttcacct
    13441 attatattca attcattatc atcaataata aatctcccat gattttttat caatatttta
    13501 tatttgttgg tatctattgt aaaattactg tcataaggaa tataaaatct aaactcttca
    13561 tctattacca tatcacaaga aagagtataa actatttctt ctttattatt tttattttct
    13621 tgaacccacc tttcaaaatc ttcaagacaa ttttcctctt gtccaaaaac tttatgtgga
    13681 aaacttataa aacacaaaac tattatactc ataaaaataa atatctcata ctttttcatc
    13741 ttacactcct agaaattttt agctttgaaa caatcctgtc gaaacatata cttcacccac
    13801 tataattttt ccacaattat caattattgt caatttatat aagtatcttt attaattgtt
    13861 ataaataaaa agctacttac taaagcaaac agacgtttca ctttaataag tagctatatt
    13921 tttataacaa aatagttata atattagaac ttccttcatt tacaattttt tgtaaagtct
    13981 tttgcatttt aactcttatc tcttctggca tagtatataa tttactttgt aattgctctt
    14041 ttactaagtc atgtagtgac ttaccaaaca tatttgattc ccataaatct gctggttgct
    14101 cttcaaatac ttccataaga tattttatca tctcttcacc ttgattttga tttccaacta
    14161 ttggagaaac ctctgtagat atatctgctt ttataatatg aagtgatggt gcattggctt
    14221 ttaacttgat accatattgt tttccttgtt tcattatttc tggttcttcc agactaagtt
    14281 cttctaaaga aggagctact actccatatc cttttatctt ggcatctatt aaagcacttt
    14341 ctattttatc atactcattt ttaactttag atagtctagt tatcagacta agcagttgat
    14401 aatctccttc aattttaaat ccactttttt cttcaaggac attatagaat aaatcttgct
    14461 ttgtctgtaa atcaatattt ataacacctt ctcctagctc tacattatca acacctgtat
    14521 cttccaaaaa ctctaactca gaaaatcctt gtataatacc ttctatatcc cttattttac
    14581 ctatatctat aatactttgt tttaatgttg ttattatgct actttttatc caatgatttc
    14641 tttcaagacc ttcaacccat tttggtaagt tgattcttat ttctgtaagt ggaaaatcat
    14701 ataatacagc ttccataacc tcctctatat cttcttcttc catttcaacc acattcattg
    14761 gaagaactgg aacttcatac ttttcttcca attcacttct aagcatactc gtttcctcac
    14821 tttttggact taatgtattt aaaaccactg caaatggttt tttaagattt ttcaattctt
    14881 gtataactct ctcttctggt tctacatagc tttttctgtc aattccagta actgaaccat
    14941 ctgtcaagac aactatccct attgtagaat ggtcttttat aactttttta gttcctatct
    15001 ctgctgcctt ttcaaatgtc atagcttctt gtgaccaggg agtggatact aatctttgtt
    15061 ttccaccttc ctcatgccct aaagctcctt caactatgta tccaacacaa tctaccattc
    15121 ttacttttaa agatactgtg tcttttattt ttatttctac accatctgct ggtacaaatt
    15181 ttggttctac tgtcattatc gttttacctg aaccactttg gggtatctca tctcttgttc
    15241 tatccttttt aaattcattg tctatattag gtataaccaa cttttccata aattttctta
    15301 taaaagttga ttttcctgtt cttacaggtc caactactcc aatatatata tccccctgag
    15361 tccttttgga tatatcttcg tatatgttat tattcatatt attcctccta tacatctaat
    15421 cttattaaat tatatttaga gttaagggtt aatattctta aaatagaaaa ataaagacca
    15481 gcaatattaa tttaatttac atatagaaat ttgtaaattt caatgtaaat taaattaata
    15541 ttgctggtcc aaatagtagt gtattatatt aactgacttt agtagcattt tcatttgaat
    15601 tattattcat ctcaataagt tctttacgtc tttcttttgt catgttcatc catataaatg
    15661 taaacaccaa tccaattatg gctactatag taagaactat gaagtagtac ctatatccag
    15721 ttacacccgg aaatgcatcc aatatcttcc ctgctgctaa aggacaaagt acttctggaa
    15781 gatatcctaa tgtagcaact attcctatag cagttcctga tattgataat gaataattac
    15841 cttcttcaag taacgaatag tgtaaacctt gaattgcata catagataca tatattgcaa
    15901 cacatccaat caatagtata taaatcatag atgattttgt tggtgtaaat attacaccta
    15961 aaagacctgc aatcataagt ataaatccat aagatataat ctttgatgaa ccaactttat
    16021 ctccaagtaa tccagaagca gctgatgcta ctggtctaca atactgagct aatattgaga
    16081 ttgcagatgc aaacaccaca gtagctccaa atgcttcagt tgaataaggc gtaaaataat
    16141 aaaatgacat attagctgaa tatgaacaaa acattattat tgttattatc catgtatgag
    16201 gcatttttat tactgcaaaa aaggcttctt tatcaaattt tgctgatacc tctttttcat
    16261 tatcctttaa tttgaaaaat actaatactc caactattac atttattgcc gagtataata
    16321 ctataatagc tgtaagacca gatttatcac ttacttttga tgaaatatac ccaaatagta
    16381 tcaatactag agatagatga attgcattgg taattccacg accactttcc ataaatccaa
    16441 aagcttttcc ttgttcattt tcagatgcta acattctaac agcttttaca agagctggcc
    16501 aaaaagtaag tattgttgtt attccccata aagcatggat taaaaacaca acaatatatg
    16561 gaggataaag tgttaataca agaccagata ttcctgtaat aattaaagaa cctgaaagca
    16621 attttctggc tgaaatacgg tctgctaaaa aaccaccaaa aaggtatgat accattgcaa
    16681 gtagaccata aatgcttcct aagcttccca tttgtgtatt tgtaagatta aatgcttgtg
    16741 caaaagtatt ataataataa ttctttaaat atggtaaagc atatataaat gctcctgcta
    16801 caaagagtat tatcaagact ttgaaatttt tccaaatgct tggtttattt acttcaactg
    16861 acatacttcc tccttatgta aatacctgta aattttcatg ataattaatt attgcttatt
    16921 gtattgttta ttcaatattt cggaatattt attaatatat taggttaatc tatcaataat
    16981 ttatggtgag ttatgagcta aaagtaaact cttttagctc accataataa ttatattttt
    17041 atttatacta acgtccagag ttattagata tctttttttc atagttcttc catagcatta
    17101 aacctgttat tataacaaaa agacctcctc ctatcatttc aatctcatag attatattat
    17161 cttttaaagt ctccataact ggtgccgttg agaaaagtat gccaactaca gttactacaa
    17221 gtaccattct ggcaatattg atacaaactg aatcactttt tgcaattgaa tatggtcttt
    17281 cttcatttgg tcttgtcttt ctaagtctaa tataagatgt aaaaagcaag acatatggaa
    17341 ataatgctgt taatgctgtc atagtaacaa gaacattgta tatattatct actgatggta
    17401 ataagtttgt tcctacaaga agtagactca ctattatagc ctgaactatt actgcatttg
    17461 atggtatatt gtatttattt gtaacagtca attgcttagg aaatacacct tctcttacat
    17521 ttccaaaaag catctttact ggacctgcta tataaagtat taatgctcct agtactgata
    17581 aagttattcc aaatgctact attcttataa accaacttcc tatacctaaa tcttgtgcaa
    17641 ctttagctaa tgcatctagt atccctgtag atgctgcaat tttatctgga ggaagtatca
    17701 ttgttattgc tactgaacct aagatataga ttcctcctat taaaacagcc acagttaata
    17761 tagctttagg aaagtttttc ttagcattat ccatttcggt tatgaaatta gcagtcgttt
    17821 cagctcctga taatgcaaat attatagcag atatagaaac taatgaattg gcatctattt
    17881 ttggtattat attttggatt gtatatatcg atgctgatgg agcttttttg agaataacta
    17941 cagacataaa tgccatcact ataagtaata tagcaggaat tgtagaacct aatgctcctg
    18001 tattcgtaaa aaattttcca aatgccatcc cacgagtact tactaaggac aacagccaaa
    18061 atattatgag tgaaagtgct aaaataaaca tcttattgtt tgataaatct ggatttccaa
    18121 gcatatatgc aatatttata gctaaaaatg tcaagaaaga agaataccaa aatatctttg
    18181 atgtccaatt tagccatgac accataaatc cccacttctc tccataagct tgtttaaccc
    18241 attctccaag tccaccatct tttggatatg ttgcagccaa ctctgcacat ataagtgatg
    18301 caggaacaaa gaatataaaa gcaaacaata accaaactgg tatagaacca agacccaatc
    18361 caaaactaga ggcagtagat ttagctatcc atctaatccc aaatagtgct gatatattca
    18421 taaatactaa atcccaaaat ccaattttct ttttcactgt aattcctcca atttcaaatt
    18481 tattcaaact catacactat gtattgattt aaatatcaag tataagttct tcaacacttc
    18541 ttggataatt agttataatt gtagcaccat tcttacctac tattgctgta tctgagtgtc
    18601 taaatcctcc aactccaggt atatatatcc ctggttccat agaaaatacc attccttctt
    18661 ctaatattaa ttcattgtca aatcttaaat atggctcttc atgttctgat aaacctaaac
    18721 catgaccaat tctatgattt gaatataact ctagaccata ctttgcaaca acagctcttg
    18781 ctgcttcatc caccattctt gctggtatac cagcttttat tgtatctaat ccagcttgtt
    18841 gagcctctac tgcaatctta aacacttctt tttgtctttc tgttggttta cctataataa
    18901 aagttctttc attttctgct ctatagtttt cataccaaac ctgtctacta tgtattacta
    18961 tatctcctct ttgaagtatt ctagtattgg aatctaaatg aggctgtgct gttctatcaa
    19021 tccctgaaca agtccagttt gcaaatccta tatatgtatc aggataattt tcagaaacat
    19081 attttagtaa agcattatcc cctgcaacgt caaactccaa ttcactcatc ccaactctaa
    19141 cattttctaa agaccctttt attcctatat cagataggta acctgctatc ttcaaaaatt
    19201 ctatttcttt tgcatctttt acatatctca tttcaaatat tttacttcca acatctttga
    19261 tagcaaatga cttatcagtt atatactctg aaaagcttgc agatactata tctttttcta
    19321 ttccaacagt tgttccttta gggtatttat ttaaaattat atctaagtaa tgtttatgtg
    19381 atattccatg ttctttcatt tctggtattt cataatacac atatacatta tcaacttttg
    19441 caacttcttt agcatgtaat tcttctaatc caggtactat taattctatt ttatcttgag
    19501 ttactactgt tacaattggt ctagaataag ttattgcttt aaatccacta atataaaatt
    19561 gattgtctgg ttcaaatatt aaagcaattt caatgctttt ttcattcatg tactttctca
    19621 atctttctag tctcatatta tttgacataa tgcacctctt tcttaatatt cagataattt
    19681 attcaataca acaaggatga atatattatt ttaaaattct taatattttt agtacttgtc
    19741 gtatttgtat gtaaattaat tttatttgtg attattttat cataatcatg attattttgt
    19801 caatcatgat tttattttaa aaaatttttt atattctagt gattttaaat tatctaaaat
    19861 ataacgagta tacattgtat tttgtaatat tattcttgat ttatggtaag tttaatgata
    19921 aaatcacgat tatataaact atattgatta tttgtaggtg aataaaatga caaataaaga
    19981 attacaaaag aaaaggatat tgatgtattt catagaagct gctcaaaata ttatggaaaa
    20041 cgaaggtata gaaaatatta cacttcgtaa ggttgcagat atggctggct ataatagttc
    20101 aactc
//
LOCUS       DAEMHW010000058        19918 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEMHW010000058 DAEMHW010000000
VERSION     DAEMHW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.058"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            354..1184
                     /locus_tag="KPK67_001763"
     CDS             354..1184
                     /locus_tag="KPK67_001763"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBG2458749.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            1188..2069
                     /locus_tag="KPK67_001764"
     CDS             1188..2069
                     /locus_tag="KPK67_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBG2458750.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            2099..2716
                     /gene="gmk"
                     /locus_tag="KPK67_001765"
     CDS             2099..2716
                     /gene="gmk"
                     /locus_tag="KPK67_001765"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBG2458751.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            2719..2985
                     /locus_tag="KPK67_001766"
     CDS             2719..2985
                     /locus_tag="KPK67_001766"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBG2458752.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            2990..4189
                     /gene="coaBC"
                     /locus_tag="KPK67_001767"
     CDS             2990..4189
                     /gene="coaBC"
                     /locus_tag="KPK67_001767"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBG2458753.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            4428..6953
                     /gene="priA"
                     /locus_tag="KPK67_001768"
     CDS             4428..6953
                     /gene="priA"
                     /locus_tag="KPK67_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBG2458754.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            6971..7411
                     /gene="def"
                     /locus_tag="KPK67_001769"
     CDS             6971..7411
                     /gene="def"
                     /locus_tag="KPK67_001769"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG2458755.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            7426..8355
                     /locus_tag="KPK67_001770"
     CDS             7426..8355
                     /locus_tag="KPK67_001770"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBG2458756.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNIIETGLVLG"
     gene            8365..9117
                     /locus_tag="KPK67_001771"
     CDS             8365..9117
                     /locus_tag="KPK67_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBG2458757.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            9186..9887
                     /locus_tag="KPK67_001772"
     CDS             9186..9887
                     /locus_tag="KPK67_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBG2458758.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            9949..11274
                     /gene="rsmB"
                     /locus_tag="KPK67_001773"
     CDS             9949..11274
                     /gene="rsmB"
                     /locus_tag="KPK67_001773"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBG2458759.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            11282..12313
                     /gene="rlmN"
                     /locus_tag="KPK67_001774"
     CDS             11282..12313
                     /gene="rlmN"
                     /locus_tag="KPK67_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBG2458760.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            12333..13085
                     /locus_tag="KPK67_001775"
     CDS             12333..13085
                     /locus_tag="KPK67_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBG2458761.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            13102..15117
                     /gene="pknB"
                     /locus_tag="KPK67_001776"
     CDS             13102..15117
                     /gene="pknB"
                     /locus_tag="KPK67_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBG2458762.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKTAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            15225..15962
                     /locus_tag="KPK67_001777"
     CDS             15225..15962
                     /locus_tag="KPK67_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG2458763.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKANISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            16060..16944
                     /gene="rsgA"
                     /locus_tag="KPK67_001778"
     CDS             16060..16944
                     /gene="rsgA"
                     /locus_tag="KPK67_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG2458764.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLVLEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            16949..17620
                     /locus_tag="KPK67_001779"
     CDS             16949..17620
                     /locus_tag="KPK67_001779"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG2458765.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIVDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            17629..18270
                     /locus_tag="KPK67_001780"
     CDS             17629..18270
                     /locus_tag="KPK67_001780"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBG2458766.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            18401..19030
                     /locus_tag="KPK67_001781"
     CDS             18401..19030
                     /locus_tag="KPK67_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG2458767.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            19085..19741
                     /locus_tag="KPK67_001782"
     CDS             19085..19741
                     /locus_tag="KPK67_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2458768.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT         8101 a         1953 c         3811 g         6053 t
ORIGIN      
        1 agatattact aaggaagatt tagactatgt tttaaaaaca cttaaagaat taataggtgt
       61 atgttaatag atatctacta gaaggattat agctagttac cagatgttta attaaaaaat
      121 tcatagacag tttaaataaa acatacataa aattcatata gaaatattaa tatataaaca
      181 tagaaagaac tagcttaaaa acataatata attaccctaa ttgtaatggc aactctccct
      241 cacatcctca aattatagtt gtcattacaa aacttttccc ataaattata agtttatggg
      301 aattttttta tgttataata gtttaagcgt taatttacga aggagcgata cttatgaaat
      361 tttggaaact acatggaata ggaaatgatt ttatagcaat agatggaaga ttcgaccaga
      421 taaatccatc tgattatact gctttagcaa aaaaagtatg ccatagaaga ttttctgtag
      481 gagcggatgg attgttagtt gtgaaagatt caaaagttgc agatgtagaa atggtatatt
      541 ataattctga tggttcaaga gcagctatgt gtggaaatgg ccttagatgt ttttgtaaat
      601 ttgtttatga taattgtata gttaaaaaag aaagttttac agtagataca ttagatggta
      661 taaaagatat aaaaataaat cttaccaata gagaaataaa ctctataaga gtcaatatgg
      721 gaaaaggaag ttttatagct aaagacgttc ctgtacttac aaataaagaa cgttttgtgc
      781 aagaaagtat aaaggtttta gatagggagc ttaaagttac ttctatgttg atgggggttc
      841 ctcatacagt agttatagta gatgaattag atgtagatga tgtttgtaaa tatggaaaag
      901 agattgaaaa taataaaata tttcctgaaa aaacgaatgt aaattttgta aaggtagaag
      961 ataagtataa tatacatgtt tatacttggg aaagaggttg tggatatact ttaggttgtg
     1021 gtactggtat gactgcatca gcaatagtgt gcaatttact taatatggta gaaagtagtg
     1081 taaatgtaac gtctcaaggt ggaacagtta aaatagatgt aggagatttc tcttatatga
     1141 caggtccagc agtaaaaata tgtgaaggta ttttggaggt ttaattaatg gctataagta
     1201 tgactggttt tggaagagga gaatacaagg atgataatta ttacttttta gtagagtgta
     1261 aaacgataaa tcataagtat tcagatataa acatacgtct tccaagaaaa atttcatttt
     1321 tagaggataa agttagaaat ttagtaaaaa attatgtaaa aagaggaaga gtagatttat
     1381 acataaagtt tgacttatta ggcaaagaag atgtaaactt aaattttgat gagggattag
     1441 catctcagta tatagacata ctaaaagaaa taaaaaataa atttgatatt atagatgata
     1501 ttagcgttat gaatgttgca aagtttcctg atattgtgaa aatagaagaa aaggaagaag
     1561 atgaagattt attgtggtca atgttgaatc aagcagttga agatgcatta atcaaattaa
     1621 gagaaatgag aagtgaagaa ggtaaaaaat tagctgaaga tattgccatg agatgtgatt
     1681 tattaaaaaa tcatatagaa gagattgaaa aatattcaag tagtgttgta gaagattata
     1741 gagaaaaatt gaatttgaga ataagtgaat tactggatga cccaagtata atagatgaga
     1801 atagattagc acaagaagtt gcaatatatg cagataaaag cagtataaca gaagaaatag
     1861 ttagatttaa aagtcatata gggcaactta aaaatactat atttaaagac gactctatag
     1921 gtagaaaaat agatttctta atacaagaga tgaatagaga aactaacact ataggttcga
     1981 agtcatctga tataaatata actaatttag tagtagaagt aaaaagcgaa ttggaaaaaa
     2041 taagagaaca aatacaaaat atagagtaag aatacaatat tagaagggga atgcttaaat
     2101 gtttgtgaaa aaaggattat tgctagttgt atcaggacca tcaggtgcag gaaaaggtac
     2161 tatttgcaaa gaactactta aagaaaatga tacaataaaa ctttctgtat cagctactac
     2221 aagaaaaccc agaactggag aagttgatgg agtaaactat ttcttcataa gtaaggaaaa
     2281 gtttgaagag atgatagaaa aaggtgagtt tttggagtat gctcaaattt atgataattt
     2341 ttatggaact ccaaaagcgg caataatgga atgtttagaa aaaggacaag atgtattact
     2401 ggagattgaa atgcaaggag caaaacaaat aaaagaagtt tgcccagagg gagtatttat
     2461 atttgtacta ccaccatcac ttgaagaatt aaaaaataga attgttggaa gaggtacaga
     2521 aacagaagct gaaatagaga agagatttag ctgtgcatat gaagaaatta aaatgattaa
     2581 ggattatgat tattttatat ttaatgaaga tgtaaaaaca tctgctaaag aaatagaagg
     2641 tataatatca tctgaaaaga ataaagttag cagatataaa aatattataa tagagaaatt
     2701 taaggaggaa ttataattat gttaaaacca tctatcaatg aggtactaga aaaaatagac
     2761 aacagatact atttagtagg gactgtttca aagagagcaa gaaaattaat tgatggagaa
     2821 gaaccatatg tttcaaataa aactaaggaa aagcctgtat gtgtagcaac taaagaagtt
     2881 gcaagtggaa aaataacata tagattattg acagaagagg aaatagagat agaagaagct
     2941 agacatcatg ctgagcaaca ccaacaaatt tcagaagagg agtaaggtca tgctaaaaga
     3001 taaaactgtt gtaattggca taagtggcgg tatagctgca tataaggctt gtgatgttgt
     3061 aagtaagtta aaaaaactaa atgcaaatat ccatgttata atgacaaaat cagcaacaga
     3121 atttgttaaa ccacttacat ttcaatcgct gagtcaaaat tatgtagtag aagatatgtt
     3181 tgaagaacca aaaacttggg aagttgaaca tatatcatta gcaaaaaaag cagatttatt
     3241 cttaatagtg ccagcaagtg caaatgttat aggcaagatt gcaaatggta tagctgacga
     3301 tatgttgaca actactgtta tggcaacaaa agcacaggtt cttatagcac cagctatgaa
     3361 tactaatatg tatgaaaatc caattgttca aaaaaatata caaactttaa agagctttgg
     3421 atataaattt attgaaccgg aaagtggaag gcttgcatgt ggagatattg gaacaggtaa
     3481 actagctaat ccagatataa tagtagagga agttttgact aatcttacta aagagcaaga
     3541 tttaaaaggt caaagtatga tagttacagc tggtccaact gtagagagta tagacccagt
     3601 aagatattta actaataggt cttctggtaa aatgggatat tctatagcaa gagaagctat
     3661 agctagaggt gctgatgtaa cattaatatc aggtcctaca aatctttcta ttccacaaaa
     3721 tcttaaaaaa tttgttcaaa tagagtcagc acaagatttg tatgaagcag tagtatcaaa
     3781 tttaagtgaa aataaaatag taattcaaag tgcagcagtt gcagattata aaccaaaaac
     3841 ttattctgac aaaaaaatta aaaagaagaa tgatgattta tcaatagaac taagtagaaa
     3901 ttatgatatt gcttatgaaa taggaaaaat aaaagaagat aagatactaa ttggatttgc
     3961 agctgaaact aatgacctta tagaacatgc caaaggaaaa ataaaaaaga aaaatttaga
     4021 ttttattgtg gcaaatgatt taactaaaga aggtgctggt tttagagctg atactaatat
     4081 tgtaaaaata atagatgtag agggaaatat tactgagtat cctaagatga aaaaagaaga
     4141 agtagccaat gttattttag ataaagttaa agcgttgctt gagaaataag acctttttta
     4201 aatatgatat ttgtgtattt tatcacagct aaagtggctg aaagctgcaa tttaatttta
     4261 gcgagtaaaa actcgcaaat gtcagttaaa ttgtagtttt cagcatttat tttgtcataa
     4321 agtataaaat ataattaata gttaattatt aaaataaagt taatattaat aacttgaaat
     4381 tgcttataaa ttctgagatt ttaatatctt gtaaagtata ttaaaatatg aacaatatat
     4441 taaatttaag tagaggtttt ataatgaaaa aatatgcaaa ggtaatagtt agaagtaata
     4501 ctatatatac agataattta tttacatatc aaattcctgt atttttatct gatgtaataa
     4561 aaattgggca cagaatatta gtaccatttg gaaaaggtaa taaaccaaca gaagcctttg
     4621 tatttcaatt tacagatagc ttagatgaaa aaataaaaat aaaagaaatt attgatatat
     4681 tagatgaaaa tccgatattt agaaaagaag atttagagtt ggtttattgg atgaaaaata
     4741 gatatttgtg tacttatata gaatgtataa atttgatata tccaaaggga tacaaattaa
     4801 ataattataa agtagtccta ttaggtgaaa gtttaagtgg attaaatgat attgaattga
     4861 aggaaaaaat atcaacttta agtgataaaa atagagaaat agtagagaaa gtgattgata
     4921 gtaaaggtaa aattaaggtt gataagttaa aatatatacc aaacttaaat agttctttgt
     4981 acacaatgaa taaaaatgga attataaaat tatgttggga atataaaaat cacaaaaatg
     5041 aaaagaaagt atgttatata tctttgagtt tagaaagtga taagattgac gattatatag
     5101 agcaaaataa aataaatgtg ggaagtaaac aaaaagagat attaagtttt cttaaaaata
     5161 atgaaaatgt agagataaat gatttattgg atttattaaa tgcttcaaaa caaagtataa
     5221 attctctaag taagaaaaag ctaataaccc tagaatttaa ggattattat agagagccaa
     5281 aaagtattta caaatccgta ctaaaaagta taaaattaaa caatgagcaa caagaagcag
     5341 ttgatgagat aaagtcgaat atgtttgtag atgataaaaa accatactta attcatggag
     5401 taactggaag tggaaaaact gaagtttaca tggaaataat agaatttgct ctaaatcaag
     5461 gcttagacag tatattctta gtacctgaga tagcattaac tcctcaaaca atagataggc
     5521 taaagagtag gtttggagat ttagttggag tgttccacag taaactatct gaaggagaaa
     5581 aacatgatgt ttacaaagct gtcaaagcag gtaaagtaag agtgttaatt ggagctaggt
     5641 cagctttatt tgcacctttt aattccttag gcttaataat aattgatgaa tgtcatgagt
     5701 catcatataa gtctgagaag aatccaaaat ttaatgctat agaagttgct agatttatgg
     5761 cattaaaaaa taatataact cttattttag gttcagcaac gccctcaatt gaagagtatt
     5821 atagggcaaa atctggagaa tacaaactta taaatataaa aagtagagca aatgataagc
     5881 ctcttccaaa cattgaagtg gtagacatga aagatgagtt agataaagga aatagaagta
     5941 tatttagttt gaaattacaa aaagaaatac gttatgcgat tgaagaaaat aatcaagtaa
     6001 tactattttt aaacagaaga ggttatgcaa attttgtatc ttgtagaaag tgtggatatg
     6061 tgtttcaatg tgaaaattgt gatatatctt taacttatca taagaaaagt aatactggaa
     6121 gatgccacta ttgtggatat gagaaagaaa ttccaaaaga gtgtcctgaa tgtaaaagta
     6181 cttatgttaa gccatttgga gttggaactc aaaaaattga agaggaatta aaatatattt
     6241 ttccagatat aaaaactttg agaatggata aagatacaac ttcaaaaaaa ggtgctttag
     6301 atgagatact aaataaattt aaagataaag aagcagatgt acttattgga acacagatgt
     6361 taagtaaagg gctggatttt gagaatgtaa ctttagtagg tattttgtct gcagacatga
     6421 tattaaattt tccagacttt aaaagttttg aaactacatt tcaattgata actcaagtct
     6481 ctggaagagc agggagagct gataaagagg gaaaagtagt acttcagact tatgacactg
     6541 aacactatgc tattaagcat gctattgaat atgattatga gggtttttat gaagatgaga
     6601 taaaaataag aaaagcattt ggatattctc cttttaataa tatgttaagt gtagttgtta
     6661 gtggtgaaga tgaaagatta gtgataaaaa atataaaaaa tatgcatgca tcattgattt
     6721 atttattaga aaaaaggggt ataaatgatt taggatttat attaggtcca aatccatgtt
     6781 caatatcaaa aataaatcaa aattatagat ggcaaatact ctttaaagat gaaaatattg
     6841 aaattaatct cttaaagggt ataataaaat atatatgtat aacaaaacga gatttgatat
     6901 ttgataagaa tatcaatgta tctatagata ttaatccaaa cagtgtatta taaatttagg
     6961 aggaattata atggctttaa gacaaatagt tcaaatagga gaaccagtat taagaaaaaa
     7021 atcaaaaaag gttgaaaaaa ttgatgaaaa gataatacaa ttattagatg atatggcaga
     7081 aactatgtat gatgcagatg gtgttggact ggctgcacct caagttggaa tattaaaaag
     7141 agtagtagta atagatattg gagaagagct tatagaactt ataaatcctg aaataataga
     7201 aacatctggg gagcaaatag atgaggaagg atgtttaagt gttgttggag aagcgggaaa
     7261 tgttagaaga ccaaattatg taaaggtcag agctttaaat agaaatggtg aaacaataga
     7321 gttagaggga gaagaacttt tagctagagc attttgtcat gagatagacc acttagatgg
     7381 aattttattt gttgacaaaa tagaaaagta gtaggagttg atttgatgaa aatagtattt
     7441 atgggaactc ctgatatagc agttccatgt ttacaaaaaa taatagatga gaagtatgaa
     7501 atattgggtg ttgtaactca accagataaa ccaaaaggaa gaggtaaaaa actaggtatg
     7561 agcccagtaa aagaactggc aattgaaaat aatatacctg tatatcaacc agtaaaggct
     7621 agagataaag aatttataga taaaattaaa tctttaaatc cagatgtaat agtagttgta
     7681 gcttttggac agatacttcc aaaagaaata ttagagattc ctaaacttgg atgtataaat
     7741 gttcatgttt ctttacttcc aaaatataga ggtgcggcac ctataaattg ggtaataata
     7801 aatggtgaag aaaagactgg tgttacaact atgtatatgg atgaaggatt agatactgga
     7861 gatatgatac taaagacaga agtaaatctt gatgaaaata taacagcagg agaacttcat
     7921 gataaaatga tgaatatagg agcagaaact ttaaaagaaa cattaagatt aattgaagaa
     7981 ggaaatgctc caagagaagt acaaaatcat gaagaatttt cctatgcgcc aataatgaat
     8041 aaatcattag gtaacataga tttttcaaaa agtgccagag aaatacacaa tttagttaga
     8101 ggagtaaatc catggccaag tgcttatact acctataatg atgtcataat gaaagtttgg
     8161 aaaactaaag tgttagatga aaaaagcaca aaagatgtag gtacaataat tgatgtaagc
     8221 aaagatggga taaaagtaag tactatagat aatgtacttt taattgaaga aattcagatg
     8281 ccgaataaaa agagaatgtt ggttggtgaa tatataaaag ggaatattat agaaactggt
     8341 ttagtattgg gttaggtgaa aagtatgaca gatataaaga agtatttaac aagtgttggg
     8401 attttgattt taatattagg aattttgata ggagttgtta atttttttat aataagttta
     8461 actcaaatat tctccttgat gataaatatt ataataatat cactaatact tataatacta
     8521 tgttcaacag cagttactta tagagtaatt aaaggaaagt atgttaactc aaatataatg
     8581 aaaataaatt ttaatattgt cagtggatta tttcctatta tatctttcat tgcatcatca
     8641 tttggtatgt caaaaagtga tattagaaga atatatataa agttaaataa tgaatatata
     8701 tacagtaata aatataattt taatccagag gatataataa ttttaatacc tcattgtatt
     8761 caagagaata gttgtaaatt aaaagttact aatgatatag ataattgtaa agaatgtgga
     8821 agatgcaaca taggagaatt aattaagcta aaggaaaaaa ctaatgtaaa aatatttgta
     8881 gctactggag gaaccctagc tagaaaaatt ataatggaca ctaaacctaa agctgttgta
     8941 gctgttgcat gtgaaagaga tttgacatct ggaatacaag atattaagaa aattcctgtt
     9001 ttaggtgtat ttaacaaaag acctaatgga ccttgtgtaa atactaatgt agacatgatg
     9061 gatattgaaa aagctatagg ctttttaaca ggaaaaaata tattagtttg taattaaact
     9121 gtttttaagt taagtgaata ttaagaggat gtataataaa aatatatttt aggagagtgt
     9181 tatttatgta ccctgtatat ggcggttttt ggggatttga cccaacaatg gttgtattaa
     9241 ttccagctat attacttact atatatgcac aatttaaagt gagttcaaca acaaataagt
     9301 atttaagagt aaatacacgt cgaggatata ctggagagca aacagcaaga agagtacttg
     9361 attcaaatgg tttgtatgat gtaaagatag aaatggttag agggcatcta agtgaccatt
     9421 atgacccaag aagaaaggct gtaaggttgt cagaagatgt atattatgga acatctataa
     9481 cttctgttgc agtagctgca catgagtgtg gtcatgccat acaacatgct aaaggttatg
     9541 cacctcttca aataagaagt agtttagtac cagtagtgaa ttttgcttca agtatatctt
     9601 ggtttttaat atttttagga tttattatgg ctggtccatt tttaaaaatt ggtatactat
     9661 tgttttcggc ttcagtgtta ttccaaataa taacattgcc tgtagaattt aatgcttcaa
     9721 gtagagcaat cgttcaactt gggaatttag gtattataga tgaaagtgag tcaagacaaa
     9781 gtagaagagt actttcagca gcagctttaa catatgttgc agcagcattg gtttcaatac
     9841 ttcaattact tagattgtta ctaatagctc aaagaagaaa tgactaaaag taaagtgggt
     9901 tgtttagaaa caaccctttt ttatgtttaa taaaagggag aaatttatat ggatgcaaga
     9961 gaagttggat ttaaagtatt atgtgatata gaaaaaaatg acaattattc aaatattgca
    10021 ataaataagc attttaaaaa tttagaaata agtgatatgg atagaggact tgcaacagag
    10081 ttaatttatg gagtaataga aaataaatat tatttagatt atataatcaa taaactttca
    10141 aaaataaagg taaaaaaaat gtcaacttat gttaaaatct ttttaagaat gggaacatat
    10201 cagattttat ttttagatag tatatcagat tatgcagcag ttaatgaaac tgtaaaatta
    10261 tctaagaaat atgataaaaa atcttctggt ttcataaatg ctattcttag aaatgaaata
    10321 agagctaaag agactataat ggacataact gaggaagata gtgtaaaata cttatctata
    10381 aagtattcct ataactcttg gattataaaa aactggatag ataaatttgg acaagaattt
    10441 gctgaagatt tgttggaagc aaataatgaa aagcctagta tctatataag aacgaataca
    10501 cttaaaataa gtagagaaga gcttattgaa aaattaagtg aaatgggtat tgtatgttta
    10561 aaagttccta tggtagaaga agctattaaa gtcgaaaaaa tgaaaaatat agaaaaaaat
    10621 gaattattta aggctggttt atttacaatt caagatataa gttctatgat agtagggaaa
    10681 gtaattaatc caaaagaagg ttcatttgtt ttggatgtat gtagtgctcc tggagggaaa
    10741 tctactcact tagctacttt aatgaacaat actggtcaag tagtagctag agatattttt
    10801 gagcataaac taaagcttat aaactctact gtaaatagat tggggcttaa aaatgtgttt
    10861 gtagaaagat ttgatgcttc tgagattgat gaaaatagta taagtaaatt tgattatgtt
    10921 ttagcagatg ttccttgctc tggttttggt ataataagac gtaaaccaga aattaagtat
    10981 aagaaagaag aagagttaaa agacatcact tctatacaaa agaaaatttt agaaaatgca
    11041 tctaaatatg tcaaagttgg tggaacttta gtatatagta catgtacaat tcaagatgtg
    11101 gagaatataa atatagttac ttcttttgtt gaagaaaatc acaactttga atttgttcca
    11161 attgatacag taaatgtaga cttagacaat caagataaag gatatttaaa gatatatcct
    11221 aatattcatg gtatagatgg ttttttcata gcaaaattaa agagaataag gtagtgagag
    11281 aatggaaaag aaaaagatag tattaaaaaa ttttactgaa gatgaactta aagaatttat
    11341 gaaaactata gatgaaaagc cctttagagg aagtcaaata ttctcttgga tatataaggg
    11401 tgcaaaaact tttgatgata tgaacaatat accaaaaagt ttgagaaata aattagaaga
    11461 ggtttcatgt atagggcata tagatataga attaaagtta gaatctaagg tagataatac
    11521 aaaaaaatat ttgtttttat tagatgatgg aaatataata gaaactgtga tgatggatta
    11581 tgatagtaga gttactgtct gtgtttctaa ccaagtaggt tgtaggatgg gatgtaattt
    11641 ttgtgcatct acaatggatg gattaattag aaacttagag ccatgggaaa ttttagacca
    11701 agttattaaa attcaagaag atactggtaa gagggtatca aatcttgtat tgatggggag
    11761 tggtgagcca cttgataact ttgaaaatac aaaacaattt ttaaaaataa ttaatgaaaa
    11821 aaatggtctt aatatagggt atagacatat aactctttca acttgtggaa tagtacctaa
    11881 aatgtatgag ttggcagact tagaaatagc tataaattta gctttatcac ttcattcacc
    11941 atatgatgaa gaacgaagaa aaattatgcc tgtagcaaat gcttattcta ttgaagaaat
    12001 attgaatgca tgtagatatt atataaaaaa gacaaataga agggttacat ttgaatattc
    12061 tcttataaaa ggggtaaatg actcagaaaa agaagcaaag gcattagcta agctcctaaa
    12121 aggtatgcta tgtcatgtaa atttaattcc aataaataaa gttgaagaaa gagaatacga
    12181 aaaaccagat aaagcattta tttataaatt tagggatagt ttggagaaga acaatatacc
    12241 tgccacagtt aggatgtcta tgggttctga tattagtgga gcttgtgggc aattgagaag
    12301 aaaatacaag taatttgtaa ggaggaagtc gtatggttta tagttgtgcc tcccatatag
    12361 gaaaaataag aaaaaacaat gaagactatt gtgagggaga agttatagat acagaacatg
    12421 gtccaatagg aatatttgcc atagctgatg gaatgggtgg acataaaaaa ggtgaagtcg
    12481 ccagtaaatt agcagtagaa aatataattg attttctaaa ggaaaactta ttacaacatg
    12541 ataatgtaaa aatagactat atagatgata tattaaaaca ggcttataat aatgtaaact
    12601 caattgtgca caaaaaatct atggaagata tagaatttga aggaatggga actacattag
    12661 ttacggctat agtgtataat aatgtcttat atgtggcaaa tgttggagat agcagatgtt
    12721 atttgttaac agatgaaaaa tttgataaaa taactataga ccattccgtg gttgaagaac
    12781 ttatgatggc ccatgtcatt acagaagaag aggcaagaag acatccacag aggaatagaa
    12841 taactagagc aattggaaca gatgatatgg ttgttgtaga tatttttaag aaagaaatca
    12901 aaaaaagtga tataatactt cttgcgacag atggattaac tgggtttatt gatgatgaag
    12961 atataagaga tttaatatta gattatgaat atgagagaac tagtaatata agtgaagaat
    13021 tgataagtat ggccaatgat gtttctggta aagataatgt ttcggttata gtgataaaag
    13081 tataattagg acggtgatat tgtgggagat acaattttag gaaatcgata tgaaatcatc
    13141 aggaagattg gtgatggagg aatggccttt gtatatgagg ctaaagacag attattaaac
    13201 aggactgttg cacttaaagt acttagacct gaatttgtag atgatgatga atttctaaca
    13261 aagtttaaaa gagaggcaga agcagtagca agtctttcac atccaaatat agtaaatgtt
    13321 tatgatgttg gtgaagatgg aaaagttcac tacattgtaa tggagtttgt agatgggaag
    13381 aatctaaaag agattataca agatgaaggt atattagatg aatatactgc attagatata
    13441 acaaaacaaa tagctatggc acttagtgca gcgcacaaaa aggggattat acatagagat
    13501 ataaaacctc ataatattct catatcaaat gaaggaagag tagtaaaagt ggctgatttt
    13561 ggtatagcca aagctgtttc aaattcaaca atgactaata ttggtagtat aataggttca
    13621 gtgcattatt tttcaccaga gcaagctaaa ggtaaatttg taactaataa tgctgacctg
    13681 tattctctag gtatagtttt atatgaaatg ttgataggaa aagttccatt taggggagat
    13741 agtccaattt ctatagcact tcaacacatt aatgatgata tagactttac atcagaagaa
    13801 aaagttagga ttcctcaaag tgtaagaact acaataaaaa agcttacaga aaaatctagt
    13861 gcagatagat atcaaactgc tgaagaattg atagaagata tagagtatat agaaaaaaat
    13921 attgatttag attttataaa agaatatgat gattttgcaa ctaagaaaat tgatgaaaaa
    13981 gaaattaata aagttgtaaa tccaacactt gcaaaaacag ctcctgaaaa agtggttaag
    14041 cctgttgaag tagctgattt ggatgatgat gaagattact atgatgattt ttatgaagaa
    14101 gatgatgatg aagatgaaga agaggaagag ataatgagag ctaaaaagaa tcagagacct
    14161 aagagtactc caagtaagag aactaagaaa aagaagaaaa aacaggaaag cccaaaatct
    14221 agaagaagat taaaagtaat tgcggctgtt ttgatactga tattgtgtgc tcaagtattc
    14281 ttagcatata agtttttatt tgctggaggg tttggcaata aatctttgac tgttcctaat
    14341 cttgtaaata tgacattaga agaagctcaa agtgctgttg aaaaagaagg attatacctg
    14401 agtgtaaaaa gtgaagagta taatagtgaa gttgatgaaa attgtataat atcacagaca
    14461 cctgaaggtg gaagtacaaa tgtcaaaaag ggtgatacta taaatgttgt cgtaagtaaa
    14521 ggttcaagtc aggcatctgt tcctaatgta gtgggactta ctttgagtaa tgcaaaacaa
    14581 cttattgaag aaaataactt aaaagtggga actgttaagt atgaatacag ctcaatatat
    14641 aaagaaggta cagttttaag tcagagtccg ggtgctggct catcaagagc tcaggaaggt
    14701 gatgaggtaa acctatatgt aagcaaaggt tcagaaaaat caaatacaca gactccaaca
    14761 gtaccagata aaaaaccaac aacaccagag aataatacac caacagaacc tggttcaaac
    14821 tcaggaaaca gtggtggaaa ttcaggaggc tcaaattcag gaaatagtgg tggaaactca
    14881 ggcaataatg gaagtaactc aggaggttca aactcaggaa atagtggtgg aaactcagga
    14941 ggctcaaact caggagataa tggaggaagt tcaggaggct caaactcagg agacaatgga
    15001 ggaagttcag gaggttcaaa ctcaggagac aatggagata gttcaggagg ctcaaactca
    15061 ggagataatg gaggaacttc atcaggaact ggcgcaaaca ttggtaaaac agaataaata
    15121 ttaataaaat aagagctgtc cttttataga aaatattttc tttaaagggc agtttgtttt
    15181 gtataaaata ctgaattggt tataatataa ggagaaaagt atttatgaag aagaaaacag
    15241 ttttaattac aggaggagct agagggattg gtaaagctat gtctaaagct tttgcaaaag
    15301 aaggttacaa tgtattagta aattttaata agtcagaaaa tgaagctaaa gagttgtaca
    15361 ctatattaaa tgaaaaaaat ttttctgtaa aactttttaa ggctaatatt tcaaatagag
    15421 aagatgtaga ggatatggta gattattgta taaaagaatt tggaggattg gatgtacttg
    15481 taaacaatgc tggtgtaagt caagataagt tgtttacaga tataactgat gaagattggg
    15541 ataatatgat gaatattaat ttaaaaggaa gcttttattg ttcacaagtt gctttaaaat
    15601 acatgatatc agaaaaaaaa ggaaatataa ttaatatatc ttcaatctgg ggtatatcag
    15661 gtgcctcttg tgaagtgcat tattctataa caaaagcagg cataataggt atgacaaagg
    15721 cattagctaa agaagtagga ccatcaaata ttagagtaaa tagcatagct ccaggtgtca
    15781 ttaatactga catgttatct ggatataatg aggaagatat agacgctctt gtggaagaaa
    15841 cccctctaat gaggctagga actccagaag atatagcaaa ttgtgctatt ttcttggcat
    15901 cagacaaatc aaattttatc actggtcagg taattagtcc aaacggagga tttgttattt
    15961 aaaaaaaatg tggtattata taaaaatagg aaagaataac acgatatact actcataaac
    16021 agtatgtgtt tatataaatt ttataaaggg aggcatttga tgctagaagg aaaaattata
    16081 aaggggatta gtggatttta ttatgtagat acttataatg gtatttatga atgtaaggct
    16141 agagggatat ttagaaagca gaaaataaca cctttggttg gtgatagagt caaaataagc
    16201 attgttgatg aagatgaaaa aaagggtatc ttagaagaaa tcgacagtag agatacagaa
    16261 cttataagac ctcctattgc aaatgtagat aaagcattaa tagtattcgc aataaaaaat
    16321 cctaaaccaa acttatctct tttagataga tttatagttt tggcagaaaa agaaaattta
    16381 gaaactgtaa taatccttac aaaagcagat ttagatgata atgatacatt agaaactgtt
    16441 aaaaatatat atgaacttag tggctataaa gtcattcctg taagtaatat tactaagtta
    16501 aatatagata aagtaaaaga agaattaaaa gaaaatgtag tagtgtttgc aggaccatct
    16561 ggagttggaa aatcaagctt attaaatgaa atagatgaaa attttaaact tcaaacagga
    16621 gttgtaagtg ataaaataaa aaggggaaaa catactactc gtcacgctga acttttaaaa
    16681 ttagaatttg gtggaatggt tgcagataca ccaggtttta gttcccttgt ccttgaagat
    16741 atagaggaag ttgaacttaa agattatttt atagaatttg ataaatttaa tgattgtaag
    16801 tttggttcaa agtgtattca tgaaaatgaa cctaattgtg ctataaaaga agctgttaca
    16861 aatggagaaa tatctaaaga aagatatgat agttatatac agctgctaca tgaaataaga
    16921 caaaataata gtaggaggta ttagaaacat gattaaatta gcaccatcaa tattatcagc
    16981 agattttgca aaattattag aagatgtaag aaaagtagaa agtgcaggat gtgaatatct
    17041 tcatatcgat gtaatggatg gacactttgt accaaacatt acattaggac cacttgtagt
    17101 caaaagttta aagaaagaaa atataaatat ggtatttgat gctcatctta tgatagaaaa
    17161 tccagaccag tatattgaag aatttgtaaa agcaggatgt gatataataa ctgttcacca
    17221 ggaggcttgt gttcatctac atagaactat acaaaatata aaatcacatg gaataaaagc
    17281 tggtgtagta cttaatccag ctacaccagt agatacgatt aaacatgtac tacctgattt
    17341 agatatggta ttattaatgt ctgttaaccc tggatttgga ggacaatcat ttattccttg
    17401 tgttttagat aagattaaag agttaaaagc tattgtagat agtcaaggat taaacattga
    17461 tatagaagtt gatggtggta taagtcctaa aaacgtagcc gaagttgtac aagcaggagc
    17521 taatgtaata gtagcaggtt ctgcaatatt tggaagtgat gacattcaag aaacagttaa
    17581 tttatttaga aagaatgctt ctcttgaaga attagtttaa atttaataat gaaaatctgt
    17641 attgttttaa atggcgaaat tgaagattac gaagttacaa gagatattat aattaaggaa
    17701 tgttatgact gcataatatg tgcagatgga ggggcaaatc acacttataa aatggaaata
    17761 atgccagatt atatattagg agatttagat tcagtagaag aagaaaagat aaacttctat
    17821 aaaaataaag gtgttaaatt tgaaaaattt ccatctaaaa aagatgagac tgatacagag
    17881 ctttgtcttt ttttagctaa gacacttaaa gcaaatcata ttgatttttt tggtgcttta
    17941 gggggacgga ttgaccacac tcttgcaaat ataaagctat tatattattt aaaagaagat
    18001 ggtatatatt ctaggatact ttctgataaa gaagagatgt atatagttga aaatgaggaa
    18061 atttcattgt atggaaaccc aggggacact atttctgtaa tagctataaa tggtgatgct
    18121 aagggtgtaa cacttactgg cttagaatac cctctcgata attattatat gaaatactct
    18181 gtacctattg gaatatcaaa tgttatgttg agtaattctt gtaagattaa agtagagcaa
    18241 ggctgtgtac ttgtagttag aaatttataa aatttgattt tgacaattgg ttttatattg
    18301 tagagcagat acaatcaagt aaaatatatt tatgattgta tctgcttttt agtatataat
    18361 tataaatata taataaactt tgacataagg gggatttttt gtggtagata gtattatttt
    18421 tgatttagat ggaacattgt gggattcaac tgaagaagta tgtaaagtct ggcaggatgt
    18481 tttaagtaaa cataaggaaa tagaattaag tgtcacaaaa gatttattta gaagtcttat
    18541 ggggctttct tttgaggaaa tagcaaatcg tttatttcca aatttaagtg aagaagaaag
    18601 aatgaattta ttgaatgaat gttctttgaa agaatgtgac tatttatctg aaaatggagc
    18661 taaattattt gatgatatag aagatactct taaattacta tctaaaaaat ataaattatt
    18721 tatagtaagt aattgtcaag ctggatatat agaatctttt ttaaaagcac ataaactaga
    18781 acagtatttt atagattttg aatgtcctgg taatactggt ttgcataaag gagaaaataa
    18841 taaacttata attgaaagaa ataaattaat aaatccaatc tatgttggtg atacgcaagg
    18901 tgatgcaaat agtgctaagt ttgtaggtat accttttata tatgccaaat atgggtttgg
    18961 gaatgtagat gtatatgatt attttataga ttcatttaaa gatttgttag aacatgatat
    19021 attaaaataa gatgagttta aaaattatat taaaataata aaaaagttat ggggggattt
    19081 aaaaatggaa ttacaagata ctatttttaa aagacaaagt gtaagaaagt ttaaaaatca
    19141 agatgtatca gatgaagata ttttaaaaat gataaaagca gcaggtgcag caccatcagg
    19201 caagaatatt caaaactggc attttgttgt tataaaacgc cgtgatttaa tggaaaagat
    19261 agcagatgtg ataactaaaa agcaacaaga aatactggta gaaatggata aagtatcagt
    19321 ggataaggct aatagattta gaaaatttgt taaaaacttt acactatttt acttaaaagc
    19381 tccagtttta gtattagttt ttacaaaggt atataaccca tcaggatatt atgaattaga
    19441 gcttatagat gcacctaagg agactataga taaattattt ataagaaatc caggtatgca
    19501 gagtctaggt gctgcaattg aaaactttac actatcagca attgaacttg gatatggttc
    19561 ttgttggttg acaagtcaaa actatgcagc agatgagata gaagctgttt tagaagcaga
    19621 aactggtttt gaaaagggag agtatttttt aggtgcaatg ttagctcttg gagtgccaga
    19681 ggacaatttg aagagtccat ctaaaaaacc agtagaagaa atatgtacat ttataaaata
    19741 aaagtcaagg acattttaaa atcgaagtaa attaaaaaca aaataaatta aaaataaata
    19801 tttaaaaaat taaaaagtag gttttattga tattaatttt atatttcaaa tcattgattt
    19861 aattaataca ataagaacta ctttttttgt atattaattt taaatatatt taaatctt
//
LOCUS       DAEMHW010000059        19881 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEMHW010000059 DAEMHW010000000
VERSION     DAEMHW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19881
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.059"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            27..101
                     /locus_tag="KPK67_001783"
     tRNA            27..101
                     /locus_tag="KPK67_001783"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KPK67_001784"
     tRNA            116..200
                     /locus_tag="KPK67_001784"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="KPK67_001785"
     tRNA            210..293
                     /locus_tag="KPK67_001785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="KPK67_001786"
     tRNA            317..391
                     /locus_tag="KPK67_001786"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="KPK67_001787"
     tRNA            416..492
                     /locus_tag="KPK67_001787"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="KPK67_001788"
     tRNA            502..577
                     /locus_tag="KPK67_001788"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="KPK67_001789"
     tRNA            586..661
                     /locus_tag="KPK67_001789"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="KPK67_001790"
     tRNA            664..752
                     /locus_tag="KPK67_001790"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="KPK67_001791"
     tRNA            756..831
                     /locus_tag="KPK67_001791"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="KPK67_001792"
     tRNA            838..911
                     /locus_tag="KPK67_001792"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="KPK67_001793"
     tRNA            926..1002
                     /locus_tag="KPK67_001793"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="KPK67_001794"
     tRNA            1020..1096
                     /locus_tag="KPK67_001794"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="KPK67_001795"
     tRNA            1105..1180
                     /locus_tag="KPK67_001795"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="KPK67_001796"
     tRNA            1188..1261
                     /locus_tag="KPK67_001796"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="KPK67_001797"
     tRNA            1269..1345
                     /locus_tag="KPK67_001797"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="KPK67_001798"
     tRNA            1358..1433
                     /locus_tag="KPK67_001798"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="KPK67_001799"
     CDS             1509..1922
                     /locus_tag="KPK67_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458769.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="KPK67_001800"
     CDS             2231..5662
                     /locus_tag="KPK67_001800"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBG2458770.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="KPK67_001801"
     CDS             5904..7832
                     /locus_tag="KPK67_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG2458771.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            8285..8749
                     /locus_tag="KPK67_001802"
     CDS             8285..8749
                     /locus_tag="KPK67_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBG2458772.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            8756..9250
                     /locus_tag="KPK67_001803"
     CDS             8756..9250
                     /locus_tag="KPK67_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBG2458773.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            9266..10291
                     /locus_tag="KPK67_001804"
     CDS             9266..10291
                     /locus_tag="KPK67_001804"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBG2458774.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMNHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            10278..12725
                     /locus_tag="KPK67_001805"
     CDS             10278..12725
                     /locus_tag="KPK67_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBG2458775.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            12905..14278
                     /gene="radA"
                     /locus_tag="KPK67_001806"
     CDS             12905..14278
                     /gene="radA"
                     /locus_tag="KPK67_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBG2458776.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            14281..15351
                     /gene="disA"
                     /locus_tag="KPK67_001807"
     CDS             14281..15351
                     /gene="disA"
                     /locus_tag="KPK67_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBG2458777.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            15440..16534
                     /locus_tag="KPK67_001808"
     CDS             15440..16534
                     /locus_tag="KPK67_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBG2458778.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            16663..18840
                     /locus_tag="KPK67_001809"
     CDS             16663..18840
                     /locus_tag="KPK67_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458779.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKSSVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFM
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(19026..19796)
                     /locus_tag="KPK67_001810"
                     /pseudo
     CDS             complement(19026..19796)
                     /locus_tag="KPK67_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         8048 a         2243 c         3732 g         5858 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actgaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taaaaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagataa aaatagtgaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttagccagt ctatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttgaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctata aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcggatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagagcttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttcg ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttatt ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 attagatgga ggaaatggat ttacaggggc aaacataagt cctcactatg atagtttatt
     3421 ggttaaaaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccatta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaaaagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatataaaaa tggcacaaga
     4321 attagaaagc ttgggagcag acataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacattag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcact
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag acaatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatggg gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataactta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cggtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacggaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaaa attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gctgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtt
     7381 taaaagactc tatgcaaaaa ggtatattag caggatatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aagatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcacaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttaggtaaac
     7921 ttatacctag tttttataca tttttgatga ataaaaaaat atataatact ttcttaaata
     7981 ttttaataaa aagaaagtat ttttttatta ttatttttac aaatgtttag taataaagat
     8041 gagaaggtat atttaaaata taaagtattt aaagttctga aatctaatca tgaagcaaaa
     8101 aattagcatt tggttatcaa taaatataat aaataaaaac tcataattaa tgaaagaata
     8161 aattcatatc taaatttcta taaaaaattt aaatttatac aatttagggc tttaaaaatt
     8221 taaataatag gtgtaaaata atagtcaaag aaagtcaaaa ataaaaaggt gtgtggtgat
     8281 attaatggca actatgacag atataattga aaaatttatt aaagatttga tggaagaaga
     8341 taatagcata caaattcaaa gaaatgaact agcaaacttg tttagttgtg caccatcaca
     8401 aataaattat gtcttaacaa caagatttac aatagataga ggatattaca ttgaaagtaa
     8461 aaaaggtggc ggaggatatg ttcaaataga aaaaatacga aaaagtaaag atggacatat
     8521 tagagaacta ctaaatgaaa aaataggaag tcagatatcc tataaaaaag caaaggaatt
     8581 attagaaggt ttaaaagagt cggatttgat taacgaaaga gaattaaaat taatattata
     8641 tgcaatagat gataaatcat tgtgtatgcc aatatatgaa ttaaaagaga aggtaaggtc
     8701 taatatttta aagaatataa taataggatt atttagtata gaggagtgat aatttatgct
     8761 atgtcaaaaa tgcaataaga ataaagcatc tgtatattat aataagatag tcaatggaga
     8821 aaaaacagag atgtatttat gtagtgagtg tgcaaaagag aatacagaaa tgaattttaa
     8881 cttagatatg cctttttcaa tgatggatat attttctaat ttaggttttc aacctaaaaa
     8941 agaacttgag gaaaagctag tttgtcctaa atgcaataca acatatagtg agtttaagaa
     9001 taatgggaga tttggatgta gtgaatgtta taatgcattt agtagtcaag taaatccaat
     9061 gcttcaaaat atacatgggc atatagaaca tacaggaaaa gcacctaaaa aatcttttta
     9121 taagataagt gtagaaaatg agattaaaga actaaaagaa gatttagata gagctataaa
     9181 aaatgaagag tatgaattag cagctcaatt tagagataaa attaaatatt taaaaggttc
     9241 tattgactaa gattggagtg atattatgaa agagaacata gtcatgaaat caagagttag
     9301 gctagctaga aatttaaata actatccatt tccaaataaa ttagataaag aatgtgccat
     9361 ggaaataata gaaaaagtaa aaaatgcgtt tattaattct aacttagaac aaaaagaaga
     9421 atttgatttc tataaaattg aagatttaga ccaatctaaa aaaatgctta tggttgaaga
     9481 acatataata agcccagatt tagcagaaaa tgataaaagt gcagttatag taaaaaaaga
     9541 taaaactata agcataatga taaatgaaga agaccatata aggatacaaa ctatatgtga
     9601 tgatttaaac ttagaatatg catatagtgt agcaaatgaa atagatgatt tgttagaatc
     9661 aagtttagaa tatgccttta acacaaagct aggttatcta acatcttgtc caactaatac
     9721 aggaacagga atgagagctt ctgtaatgat gcatttacca gcattaagtc aactaggcta
     9781 tatggatgaa ctttataaaa tttcatcaca aataggtata gcaattagag ggatatatgg
     9841 agaacgaaca gaagcattgg gaaatatata tcaaatatct aatcaactta cacttggaag
     9901 aacagagtct aatattatag aaaatgtaag cggacttaca aaagatgcaa tttcaaaaga
     9961 aataaaagct agagaaatat tgcaaaaaaa attaggtata aaactagaag ataaaatatt
    10021 tagatcgata gggacacttg aaaattcaag agtaatgagt agcgcagagg caatgaacca
    10081 tctatcaaat ataaaaatgg gaatagaaat gaactatata gataaactag atttaagggc
    10141 tatagaacaa cttatgatag gtatacaacc agcacatcag tcaataatgt ataagagtga
    10201 tgatgttgag aatagagata taaatagagc aacatatatt agagaaactt tggaaaaatt
    10261 aagaggaggg atgaattgtg aacttcaata gatttacaca aagagctaaa aaggcaattg
    10321 atttagcatt tgaatctgct aaaagtctag gacacaatat tgtcggaagt gaacatatac
    10381 ttttaggtct tttaagagaa gaagaaggta tagctgctaa agttctaagt aaagttggat
    10441 ttacagaagc ttatttagaa ggcaaaatag ttgatatgga aggtaaggga gaagaaatct
    10501 cagaagatat agtattaagt ccaaggagta aacaaatact tgaattatca ggaatgtttg
    10561 caaataaatt aaaaacaaat tatattggga ctgagcatat attattagca attattcaag
    10621 aaggtgaagg aatagctaat aagattttaa attatgctgg agtaaatgat aggactttag
    10681 cccaattaac aattgatatg atgggtatta gtgataaaaa tcaatacaaa gcagaaaata
    10741 gttacacggg caaccagaat caagcagaat ctaaagtact tgataagtat ggaagaaatc
    10801 ttacactata tgcaaaacag aataaaatag accctgtaat aggtagagaa aaagaaatac
    10861 aaagggtaat acaaatatta agtagaagaa ctaaaaacaa tccagtgttg ataggagacc
    10921 caggtgtagg gaaaacagcc atagctgaag gtctggctac aaatatagcc ttaggaaatg
    10981 ttccagaaac actaaaaaac aaaactttat actctttaga gatgggttca ttattggctg
    11041 gggctaagta tagaggagaa tttgaagaaa gaattaaaga agttgtagat gaagttgtta
    11101 aaaatggaaa cataatctta tttatagatg agatgcacac aataataggg gctgggtcta
    11161 ctggagaagg ctctatagat gcttcaaata tattaaagcc agccttagca agaggagaaa
    11221 tacaagttat aggtgcaact acaattgatg aatatagaaa gcatgttgaa aaagattctg
    11281 ctctagaaag aagattccaa ccagttatgg tagatgaacc aactaaagag gactcaataa
    11341 aaatattaga aggattaaga gataaatatg aagctcatca taaagttaaa ataactgatg
    11401 atgctataaa ggcagctgta gaattatcaa ctagatatat atcagacaga tatctgccag
    11461 ataaagctat agacttgatt gatgaagctg catcaaaagt aagattaaaa gaaaatacac
    11521 ctccttcaga aataaaaaaa ttagaactag aaatagaaaa tatagataaa gaaaaagaag
    11581 aagcagtaag atgtcaagac tttgagaaag cagccaaaat aagagatgaa caaggtctac
    11641 ttaaaaaaca attagaagat gttagagaaa gatggaacaa gtcatctaaa cattcagatt
    11701 tagttgatgg agaagttatt gctgaagtag tgggcttatg gacaggaata ccagttaata
    11761 aaattcttga agaagaggct gatagacttt tgagacttga ggaaatactg cacaatagag
    11821 ttataggtca agaacaagca gtaaaatcta tttcaaaggc aatcagaagg tcaagagcag
    11881 gtcttaaaga ccctaataga ccaattggtt catttttatt tttaggtcca acaggagtag
    11941 gtaaaacaga attatctaag gcattagcag aagtgcaatt tggagatgaa aatcaaataa
    12001 ttagaataga tatgtctgaa tatatggaaa aacatgctgt gtcaagaatg ataggttcac
    12061 ctccaggtta tgtaggtcat gatgaaggtg gtcaattaac tgaaaaagta agaagaaatc
    12121 catattcagt tatcttattt gatgagatag aaaaagctca tccagatgta tttaatattc
    12181 tgttacaaat tctagatgat ggtagactaa ctgattcaaa aggaagaact gtagatttta
    12241 agaatactat tgtaataatg acatcaaatg ttggagcatc tacaattggt agacaaaaaa
    12301 ctttaggatt tagtatagct aaaggagacg aagaagaaaa atctcaatat gaaaaaatga
    12361 aagaaaatat aatgggtgaa ttaaaacaaa gattcagacc agagttttta aacagaatag
    12421 atgatataat tgttttccac tcactaaatg aaaatcatat atcaaaaata gtacttttaa
    12481 tggcagcgaa actacaagag agattaaaag aaatggatat aaaattagaa atgagtgatg
    12541 aggctgttaa attaatttct aaatctggat ttgacttaga atatggagca agacctctta
    12601 aaagagcttt acaaaaagag ttggaagatg agctatctga agcaatctta aaaggtaatg
    12661 tgaaaaaggg aagtaatgta gtagcaaaag tcaaagatga aaaaatagtt tttgaaacta
    12721 aataatttaa tagagttgca ttttaaaata ttgcaaatat aagttttata atctaaacaa
    12781 aattttaagg gcaatgaatc tataataatt cattgtcctt aattatttta tttaatcttt
    12841 acatattgtc tttcagatat gctaaaatta tagtatagaa taaaaaatta aggatgataa
    12901 aagtatggca aaaataaaaa caaaatatgt atgtcaatct tgcggatatg aaacagctaa
    12961 atggcttggt aaatgtccag aatgtacaaa atggaataca tttgtagaag aaatagacca
    13021 aaaaagcacc aaaaaagagg tctttataat agataaatct tcatctaaac cagtcagtat
    13081 aaactcaata gaaagtaaag aagaagagcg gtttacaaca gatataaatg aattagatag
    13141 agtgcttgga ggaggtatag ttaaaggctc tttagtgctt gttggtggtg accctggtat
    13201 aggtaaatca acacttttaa tccaagtatc tagtaatgtt gccaatttag gaaaaactgt
    13261 tttatatata actggagaag aatcagaatc tcaaataaaa atgagagcta aaagattagg
    13321 tattaactca gaaaatttat atatatttgc tgaaaataat ttaagtataa ttgaatctta
    13381 tttagaaagt gtaaatccag aattaataat tctagactca attcaaactg tattcagtcc
    13441 agaaatatcc tcggcaccag gaactgtcag ccaaattaaa gaaggtactt caaaattcat
    13501 gaaaatttct aaaaaaatgg gaatttcaac gttcatagtt gggcatgtta caaaagaagg
    13561 ttcactagca ggaccaaaat tattagagca tatggttgat actgttctat attttgaagg
    13621 agaaagatat aatacttata gattagtaag agctgttaaa aatcgatttg gttcaactaa
    13681 tgaacttgga gtatttgaaa tgagagattt aggattagta gaacttgata atccatctaa
    13741 aattttaatc tctgaaaaac caaaagatgt agcaggttca gtaataatat ctactgtaga
    13801 aggtacgaga ccaatgttac ttgaattgca agcattagtt tctcctacaa gttttggtat
    13861 accaaaaaga acatctacag gagtcgatta caatagagta ggtatgttat tagcagtatt
    13921 agaaaaacgt gttggtttgc aaatacaaaa tcaggatgtc tatataaaca tagttggagg
    13981 aataaagata aatgaaccct caattgattt agggatagcc atttctgtag catctagttt
    14041 tagaaatata ccgatagatg aagatatagc tgttacagga gaagtgggac ttacaggaga
    14101 agttagagca gtaagcttta tagaaaagag aatagctgag tgtaaaaaac ttggatttaa
    14161 aaagatagta gttcctagaa gtaattatga tgttgtaaaa gagaccaaag gcatagaaat
    14221 atggccagta gataacttaa gacaagctat aaatatagta cttgggagga atcaataaat
    14281 atggagaatt ttctagataa taaaaatatg ctatatgcat taaaaatgat atctcctgga
    14341 actccactta gattaggtct aaacaatgta ctaagagcta agactggtgg attaattgta
    14401 attgcaacaa acgaagatgt aatgaaaata gtagatggag gatttgctat aaatgcagaa
    14461 tattcaccat catatctata tgaattagct aaaatggatg gagctatagt tttaagtggt
    14521 gatgtaaaga aaatattatt tgctaatgca caacttatac ctgactattt tatagaaaca
    14581 tcagagacag gaacaagaca tagaacagca gaaagagtag caaaacaaac tggtgctata
    14641 gttataggaa tttcacaaag aagaaatgtt ataacagttt atagaggaaa tgagaagtat
    14701 gtagtcgaag atatatctaa gatatttact aaggcaaatc aggctataca aactctggaa
    14761 aaatataaga cagtattgga ccaagctgta acaaatttaa atgccttaga gtttaatgat
    14821 ttggtaacta tttatgatgt tgcattagtc atgcaaaaga tggaaatggt aatgagagtt
    14881 acaagtataa ttgaaaaata tgtgatagaa ttgggtgatg aaggaacttt agtaagtatg
    14941 caattagaag aattaatggg tacaaccaga atagaccaga aattaatatt caaagattat
    15001 aataaagaaa acacagaaat aaaagaactt atgaaaaagg tcaaaaattt aaattcagaa
    15061 gaactaatag aattggttaa tatggcaaaa ctattagggt atagtggttt ttcagaaagt
    15121 atggatatgc ctataaaaac aagaggttat aggattctta gcaaaataca tagactacca
    15181 acagcaataa tagaaaactt agtaaattat tttgaaaact ttcaacaaat tttagatgca
    15241 tctattgaag aattagatga ggttgaagga ataggtgaaa taagagcaac atatataaaa
    15301 aatggactca taaaaatgaa acaattagtc ttattagata gacacatatg atgaatttta
    15361 aatgaatttt actacttttt tcttgaatat aatatacact ttggtctata atagataagt
    15421 ataactaggg ggtgaagcat tgataaggaa agtaacaaga atattgttta ttttacttgg
    15481 atttacaata ggtataacaa cctatttaac attaatgaaa gattttgaaa tattaacatt
    15541 tggaaaagaa acatatggtt atatcgcagc tgtagtagca ggtataataa tagccatttt
    15601 aggatatctt atagaaccat gggttgtaaa taaagtaaaa gaaattgcta aaatagtgga
    15661 taaagaattg tcaaaatatc ctcagactga tatattgtta gggtctatgg gtcttatagt
    15721 aggatttgtt attgcgtatt tattaagtgg attagtaaat agaataccaa tagtaggtgg
    15781 aatactatcc ttactattat atttattcct aggatatctt ggaatgaaag ttgcgcttaa
    15841 aagtaaaaat gatttattta atattggaaa actaggaaga ttagcaaatc ctattaagga
    15901 taaagataag gataaagaaa ataaaaaaga agtaaaagcc attcctccaa aagtattgga
    15961 tacaagtgta ataattgatg gaagaatagc tgatatttgt aagactgggt ttatagaagg
    16021 aaaacttatt attcctgctt ttgttttgga ggaactaaga catattgcag attcttcaga
    16081 tgatttaaaa agagtaagag gtagaagagg tctggatata ttaaatataa ttcagaaaga
    16141 attaaatata gaagttgaaa taagtgagag agactttgat gatatagcag aagttgatag
    16201 taaattatta aagcttgctc aggttttaaa tggaaaagtt gttacaaatg attataactt
    16261 aaataaagtt gctcaattcc aaggtgttga agtattaaat ataaatgagt tagctaatgc
    16321 tataaaacca gttgctatac caggtgaaga tatggttgtt caagtggtta aagaaggaaa
    16381 agaggctcaa caaggtgttg catatctaga tgatggaact atgatagttg ttgatggagg
    16441 aagaaaatat atgaatgaga ctataaaagt actcgttact tctgttcttc aaactcctgc
    16501 aggtagaatg atatttgcaa aacctaaaaa ttaatatgtt caaagaaacc tataatttgg
    16561 gctgtttatt ttaaatagat ggtccaaatt tatttattta taacgataat gtttaatgta
    16621 ttcttaaaga tggggattta aatcagaaag cagggagaat ttatgagaaa aaagattata
    16681 tctttcttag caacatttat cataatatta acatcagcaa gtcagtatag ttttgcagat
    16741 gatatatcaa cacgtggaaa agtaatattt atagatatga atagaactag tatgagtaat
    16801 atgttaagaa taaaatcatt aagagaagaa ctagacaata gagggtatat tggcttgatg
    16861 aatataagag gcgataaagg aagtgatgat agaaggtcat atgctagtat gggtgctggt
    16921 ggtagagcta atgtggctaa tgaagaagat attaattttg aaagctcgag taaggataga
    16981 aatatagttt ttgaatcagc tacaggaaaa agtgcaaaag gtataaataa tttaactata
    17041 aataagtcta taaatgaaaa tttaaacttt ggagagtatg gttctgtatt agggtcatta
    17101 ggacaaagtt tatctgataa tggattaaaa tcatctgtat tgggtaattc agatataatt
    17161 gaaaatggtc aacttataaa aaatagaaac ttatgtctaa ctgcaatgga tgaatatgga
    17221 agaataccta atggaaatgt agatactata aataaaaaag acttatctat gccttatgga
    17281 atatcaactg attatgataa attgattgta gagactaaag aagcttacaa aaataatgat
    17341 gtgatttttg tggagcttgg agacacatat agattagatt tatataaacc aaatttaaat
    17401 gaaaaaacat atgagagtat gaaagataac atagaaaaaa atatagatat atatttaaaa
    17461 aatatatttt ctatggttga agaaaatgac actgtctaca tagcaagtgc atttccaagt
    17521 gatttagatt ataaaaataa aagaagactt tctccaataa taaaattaaa tggagaaggg
    17581 aaaggtatat tatcatcttc aactactaga agagaaggta ttgtaacaaa tttagatgtt
    17641 ggggttgaaa tattagacaa ctttaatata aaaaatcaaa atatggttgg aagaaaatat
    17701 gaacttatta acagagatga taataaagaa tttttgatgg atgaatatca aaagatagta
    17761 tctatttcaa gcataaggtc tacaatactg aatggttttg taagtatagt tttcttgtca
    17821 tggatagttg ctatgattgc aattttattt agaaagcata tatcaaaaaa ttataaagaa
    17881 acaacatttt ttatattaaa ggaactttta aaaataggaa tagtaatgcc actgtcattt
    17941 atgataacac caataatgaa tttcaaaaca ccattagcaa tatctttagg aataattata
    18001 ataacactta ctatatatct tataagtaaa gttttaataa agaacgattt aaaaaacatg
    18061 ctgtttttta ctggattaac catagtaatt atggttattg atgctgggtt tggctcatat
    18121 ttaatgaaaa gcaatgttat gagttatgac tgtataattg gagctagata ttatggagta
    18181 ggaaatgagt atcaaggtgt ggcaatcggt agtgcaatat ttacatttgc aattttactt
    18241 acatataaaa atataccaaa gtggtctata atagtttttt ctttggttat cttaattaca
    18301 agtgcatctc caattatggg agcaaatgtt ggaagtgcta tttctgaatg tgttgcattt
    18361 ttactattta tactattgat atataatgta aagatagatt tcaaaaaaat cgtattatta
    18421 ggtatagcag tagtatttgt attaggagtt tttgtagcaa tagatatgat tttgggaagt
    18481 aattcacatt taggcatgtt tgtaaaagaa atttacttta atggaccagg agaaataata
    18541 caaacattta gtagaaagat agagatgaat ttaaagctag ctcaaaccag tgcatgggta
    18601 aatatactgt taactggaat aggtgtaatt ttagttctta tgattaatca gattagatac
    18661 tttaagcaac tgatggatga gtatccaatt gtatttaaag gatttatggc atcaatagca
    18721 ggatgtttag taactctttt agtgaatgac tctgggattg tgtcatcagc tacagccttt
    18781 atttatgtaa ttgtacctat gataacatta agtataaatc tcacagcatt aaaagaataa
    18841 gtactgtatg aataatatgt aaatatgaac acattaattt aacttcttta tttacaaaat
    18901 aaagaagaaa gtttataaaa aataaattat aataaaaaag agtttatctc aaaattgatt
    18961 attctaatca aattttaagg taaacttttt ttattataaa gataaataac atataacctc
    19021 atattttagg tagatttttt tcttatatgt ttaggagtgc atttaaattt ttccttaaaa
    19081 attctgtaaa ataattttgt attagtaaat ccatgagctt ctaatatact gcataaggta
    19141 tcatttgtat taattaagtc tttataaata tgagaaatac gcacatcatt taaatactct
    19201 aagtaagttt gtcccatata ctgtttaaat tttctacaaa aatattctgg ttgtaagtgt
    19261 gctaccttag cgattttgtt aatagaaata ggcattttgt aattaatctt tgtatattct
    19321 aaaactggtt caaattttga caaatcaagt actgtttttt tcatattttt attacctaca
    19381 gatattttaa agtttgtata taattcaaat aaaagttcaa ataataagct tgtaaagagc
    19441 agattagctg caggaggaga actaagttta atttctctca tgttttctaa tataagttta
    19501 actttacttg aatttttttt atagttattg tcattggcat taaccttaaa gtcaaaatag
    19561 caatttttaa aatcagggat atatttgttt agaaaagaca atggtacttg caataatata
    19621 gaggtatttt gataaataca tctagttgag tgaataattc cagaattaat gaacatacac
    19681 tcattagctt ttaaattata agtgtaaccc tccatgttaa tttgtaaatc accagaagtt
    19741 atataaatta tttctaatga gttatgccaa tgatttgcta cataactacc actatcctga
    19801 acctcccatg actaaagtca cagggttcct gcttcataga attttgcata ctaaaacatg
    19861 tcttactaat tctccacagg c
//
LOCUS       DAEMHW010000060        19257 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEMHW010000060 DAEMHW010000000
VERSION     DAEMHW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.060"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            250..828
                     /locus_tag="KPK67_001811"
     CDS             250..828
                     /locus_tag="KPK67_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2458780.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            867..1445
                     /locus_tag="KPK67_001812"
     CDS             867..1445
                     /locus_tag="KPK67_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2458781.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1531..2160
                     /locus_tag="KPK67_001813"
     CDS             1531..2160
                     /locus_tag="KPK67_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2458782.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2150..4909
                     /locus_tag="KPK67_001814"
     CDS             2150..4909
                     /locus_tag="KPK67_001814"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG2458783.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGRDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            4996..6558
                     /locus_tag="KPK67_001815"
     CDS             4996..6558
                     /locus_tag="KPK67_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBG2458784.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSRAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6559..7695
                     /locus_tag="KPK67_001816"
     CDS             6559..7695
                     /locus_tag="KPK67_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG2458785.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7759..8835
                     /locus_tag="KPK67_001817"
     CDS             7759..8835
                     /locus_tag="KPK67_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBG2458786.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8813..10051
                     /locus_tag="KPK67_001818"
     CDS             8813..10051
                     /locus_tag="KPK67_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBG2458787.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10105..11193
                     /locus_tag="KPK67_001819"
     CDS             10105..11193
                     /locus_tag="KPK67_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBG2458788.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11221..12012
                     /locus_tag="KPK67_001820"
     CDS             11221..12012
                     /locus_tag="KPK67_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBG2458789.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     FESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(12127..12681)
                     /locus_tag="KPK67_001821"
     CDS             complement(12127..12681)
                     /locus_tag="KPK67_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG2458790.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12876..14078
                     /locus_tag="KPK67_001822"
     CDS             12876..14078
                     /locus_tag="KPK67_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBG2458791.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14135..15115
                     /locus_tag="KPK67_001823"
     CDS             14135..15115
                     /locus_tag="KPK67_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBG2458792.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGYENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            15363..16316
                     /locus_tag="KPK67_001824"
     CDS             15363..16316
                     /locus_tag="KPK67_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG2458793.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16306..17262
                     /locus_tag="KPK67_001825"
     CDS             16306..17262
                     /locus_tag="KPK67_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG2458794.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIVIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17259..18014
                     /locus_tag="KPK67_001826"
     CDS             17259..18014
                     /locus_tag="KPK67_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2458795.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLMGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIKELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18106..19071
                     /locus_tag="KPK67_001827"
     CDS             18106..19071
                     /locus_tag="KPK67_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2458796.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         7643 a         1953 c         3421 g         6240 t
ORIGIN      
        1 aaaataaaat atgtacttgt ttatataaat gatatataat tacatattaa acatagcttt
       61 tatacattta ataaagtatg taaaatctat gtaaaaagta agaaatataa acattttttt
      121 aattatattt taaaaatatc aagcaagttt tcttcacaaa tgaataaaaa atgttataat
      181 atgattaaag aaaaagataa ataattagca agatttgttt agctaaaatt aacaagggag
      241 cgataaatta tgggtataac attatcaaaa ggccaaaagg ttgatttaac taaaggaaat
      301 ccaggtctta aaaatatttt agtaggttta ggatgggata ctaacaagta tgatggtgga
      361 tttgactttg atttagatac agcagcattt ttaacaggag caagtggaag tgttactaat
      421 gatggagatt ttgtatttta caacaattta aaacatacat ctggagcagt tgagcatcta
      481 ggagataaca gaactggcga aggtgatgga gatgatgaac aaatagttgt tgatttatct
      541 aagattcctg gagaaataag caagatttct tttactgtta caattcatga tgcaacagaa
      601 agaagacaaa attttggaca agtaagtaat tcttatataa gaatagtaga taaagacaca
      661 aatgaagagt taataaagta tgaattagga gaagatttta gtatagaaac agctatagta
      721 gtagctgaaa tatataagca caatggagaa tggaagttta atgcactagg ttctggcttt
      781 gaaggtggtc ttgctgcatt atgtggaaac tttggaataa acttataatt tcaactataa
      841 caataaacta aagggggata ttttccatgg ctatagtatt aaaaaaagga caaaaaatcg
      901 atttaactaa gggaaatcca ggtcttaaaa atataaaact tggactagga tgggatacaa
      961 attcatttga tagtggctat gattatgatt tagatgtaag tgttttcatg gttggagaat
     1021 ctcaaagagt tgaaaaagat gaagatttta tattctacaa taacttaaaa catccatctg
     1081 gagcagttga acacttaggt gataatagaa ctggtgaagg tgatggagat gatgaggaaa
     1141 tattagtgaa tttagagaca atgccaaagc atatacaaaa gatagctgta acagttacaa
     1201 tatatgaggc tgctgaaaga agacaaaatt ttggacaagt aagcaactca tatataagag
     1261 tattaaattc tgaaaatgat gaagaaatat taagatatga cttaggtgaa gaatttagta
     1321 tagaaactgc aattacagta tgtgagatat ataagtataa tggagaatgg aagtttagtg
     1381 cagttggagc aggatttgaa ggtggcttag aagcactttg tagaaactat ggcttaaatg
     1441 tttaatttaa gcaatataat atattaaata taaataacct atacaaataa tgtataggtt
     1501 atattatata aaattttgtg gaggtttagt atgtcgatta atttatcaaa aggtgataaa
     1561 atagatttaa aaaaatctaa cccaggactg agtaacatac ttgtaggtct aggatgggac
     1621 cctgtgcaac aatctggcgg aggttttttt aaatcgttat ttggtggagg acaagctgat
     1681 atagattgtg atgcatctgt atttatgctt aatcaagaag gtaaattaag tggaataaaa
     1741 gatttaattt attttggaaa tctaaaaagt gcctgtaaat cagttttgca tacaggagac
     1801 aatctaactg gtgagggtac aggtgatgat gaacaaatcc tagttaattt ggataaagtt
     1861 ccatcaaata ttcataaatt attgtttgta gtaaatatat acaattgtgt agatagaaaa
     1921 caacactttg gaatgataga aaatgcctat ataagagttg aagaccaagg taataagaaa
     1981 gagatagcaa aatacaatct atctgataat tattctgaaa aaactactct tatagtagga
     2041 gctatatata gaaaagatgg aagttggcaa tttaaagcaa ttggtgaagg aacaaaagat
     2101 gcgggtctta aagaagtaat gcaaaattta gacaggattg agtgtgcata tggaatttaa
     2161 taatgatatc aaatattata atgtagataa agaagaagtt acaaaagact tatctgttaa
     2221 tcctgaaaaa ggtttatcag aatcagaagt caagactaga agagaaaaat atggtttgaa
     2281 tgaatttact ccaaaagaag agggtagttt ttgggatgat ttaaaggaaa gtttaagtga
     2341 accaatgata gttatcttga ttattgcagc agtagtaagt gcagtaattg gggaaactca
     2401 tgatgcaata gggattgtag gtgctatagc aataggtata gcaataggta tgattacaga
     2461 aggtcgttct aagaaagcag cagaggctct ttctaagttg actgaaaaca tagaagtaaa
     2521 agtattgaga gatggggaag tacatcaaat ttctaagagt gaattggtac caggtgatat
     2581 agtatatatt gaaactggtg acatggtacc tgctgatggt agacttatag aaagtataaa
     2641 ccttaaaatt agagaagata tgttaacagg agaatctgat gacgtatcta aaaagtgtga
     2701 tgtagtagtt tctatggaag agatagaatc taaaggagca gtagtagtac aggaaccaat
     2761 acctgcaaaa cagataaata tggtgtttgg tggtacactt gtagcttatg gtagaggagc
     2821 tctagttgta acttcaattg gagataatag tgaaatgggt aaaattgctc aaaacttatc
     2881 agaaacagat gagcaaactc cacttcagat aaaacttgga aatttaggtg gtttgatagc
     2941 caaagtttct agtgcagtag caggattact atttatattt atggttttcc aaatgataag
     3001 taagaatgta ttaaatgtag atatgtctgg aatattaccg tttttagaat caatagaccc
     3061 agttaaaaca gcatttacag tatgtgttgc gttgatagtt gcagcagtgc cagaaggtct
     3121 tccaactatg ataaatatga cacttgcaat aacaatgcaa aaaatggcta aaataaatgc
     3181 attggttacc aaaaaagaag cttgtgaaac gataggttct gtttctgtaa tatgttcaga
     3241 taaaactggt acattaactg aaaatagaat gactgtagaa gttgcatatg ttgatggcag
     3301 atatattgaa agttcagaag aggaaataaa tagttatttt gaagaaaact gtatgataaa
     3361 ctctacagct gatgtagagc acaatgatgg tgatattaag tatttaggaa gtgctactga
     3421 atgtgcattg cttttatatt acaaaaacgt agattataga caagttagaa aaaattctaa
     3481 tatagtagct caaaatcctt ttacttcaga ttcaaagaga atgacttctg ttataggtcg
     3541 agataatcat catgtgctat tatcaaaagg agcaccagaa gtattacttg aattatgttc
     3601 acatattcaa agaggaaaag acatagttcc attaactgaa aatataaaac atgagatttt
     3661 agaagaaata aagaaattgc aaataaaatc aatgagaact ttagggtttg catataaaga
     3721 aatctcacaa gctgaagaag aagcagcagt aactgctgaa agtgatgcta tgaatgtaag
     3781 tgcaatggaa aataatttag tatttagtgg ttttgttggt ataagagacc cacttagaaa
     3841 agatgttata gagtctgtaa atacagcaaa taaagctggt gtttctgtaa aaatgcttac
     3901 aggagataat ataaatacgg caagagctat aggtgaagag ttaggcctat taaaaaataa
     3961 tatgagagtg gttgaagctt catatataga tactttagat gatgaagaat taaaacaaga
     4021 gatacaaagt atttcaatcg tggcaagaag taaaccagat agtaaaatga gaatagtttc
     4081 agctctgcaa aagagcggag atgttgttgc agtaacaggt gatggtataa atgatgctcc
     4141 agcactttca aaagcagatg ttggtatagc aatgggtata tctggtacag aggtatctaa
     4201 aaacgcagca gacataattt taactgatga tagttttagt accattgtaa aaggtattaa
     4261 atggggtaga ggaatttatg ataacttcca aagattcgtt caattccaat taactgtaaa
     4321 tgtaattgca tttttagtgg ctataatatc tcaagttatg ggacaagaga tgccatttac
     4381 tacaatacaa ttattatggg taaacatcat tatggatgga ccaccagcac tagcattagg
     4441 tttagagcca gttagggacc atgtgttaaa cagaaaacct gttgatagaa aagccaatat
     4501 aatttctaag tcaatggtgt atacaatagt attaaatgca ttttatataa cagcaatact
     4561 aatgttacaa tctacattta atttcttagg agttgatgaa gcgcataagg gaacagtaat
     4621 gtttgcgtta tttgctttta gtgcactgtt taatgcattt aactgtagag agtttaatgc
     4681 agatagtatc ttccctaatt ttacacataa caaattagca ctacaaataa tagtgttgac
     4741 aggaattgca caagtaatat ttactcagct attccaagat ttctttaact ctgtagcaat
     4801 ggattttatg acatgggtaa aagttttatg tgtagcagca agtgtaattg tggttaatga
     4861 agttgtaaaa tgtataataa gactggtaaa accaaaaaag actgcttaaa acaaattttt
     4921 tagaagaaaa cagactgact caaaatatga attttgggtc agttctgaat aaacatttaa
     4981 attgggagga tagttatggg tgactcaaag gtatttgaaa aaatattctc cagccaatca
     5041 gatagaggaa attacactcc ttcaaaggga tatctaagtt attttatttc gtatattggt
     5101 ttagaagatg aagttttgta caatctcgag atatttaaaa caaaacaaaa tattgatagc
     5161 aaaaaagata ttgctttatt tactgatgtt atagcaaatc ctagtgattt tgatattata
     5221 aattatttta aaagtggact tcaaaaatat agaactagca tggaagatgt agatataaat
     5281 attttgggat ttgaagagat tgattataaa ataaagcaag caatggatag agtgttaaaa
     5341 gaagaggaaa aagaatttac taatgataga gttaaacaaa attttatagt gaaaataatg
     5401 gcatggataa aaatttatat cggtgcttta gatataaata aaaatgaagc tccaaaagta
     5461 atattttatg gagatataaa aaaacatgaa gtttatttac tcttaatatt atacttggca
     5521 ggttttgatg ttttatatct taatccaaat tcaaagagta atatagatat tttaaaatca
     5581 gaaagataca atatagagtt tgaagaagcc aacataatag aagaaaaaat ttcttttgaa
     5641 gaaagggtta ttttagggga aaaaatagat aaaagttctg taaaaaaagc ttttactgtt
     5701 ggggcagaag catcaaagcg tataagtgag gaattgttaa atgatgcagg atttataaaa
     5761 ccttggcaac tccaagatag aaaaataaaa aatctacttc taagttcaac tgttgatgaa
     5821 atatcaatat actggaatca accacttaaa ttaagaccag gatttaagtt taatgatgct
     5881 attgtagaag ctccaaattt tctttctaag attaatggaa tatataacga taagaatgaa
     5941 tatattaagt ttttagattt attaagagat tcggaaagtt caacatttat tgaatttaat
     6001 ggagatgtag atagattttc aagagctttt acaagagaag cttttagttt atccttttta
     6061 ttggattcaa aaggtgcaat agataaaaac tctgttttaa ataataaaga ttattccata
     6121 tcaacactag cattgaatca acaaattatg attttagaga aagtggaaga gttgttagaa
     6181 ggaagtatgt ttttgaatgg tttaagtgga gaagacaaga taaaaggtct atttacagta
     6241 cttcacatgg ataagaaatt tgttcatatg atgaacaatt tcgattactc acttataaac
     6301 ccaaaattga ttatatacat gtacaagagt attgtatttg ataaagaaat agtttttctt
     6361 atgcttttac ttagtaagat tggatttgat attatcatat tatgtccagg aggagaaaac
     6421 aatatagaga atgttataaa taaccaattg attgatgtac acagattgga taaaatggta
     6481 tatgacctta aattaaattc gcttgaaaat gatatacctt tacttaaaaa aatttttgga
     6541 aagaggagac gattttagat ggggataaat ataaatagtt tttcaaaaga taagaactct
     6601 gtagaggtta ctgagaattt gcctacagca gttcaagaag aaaacttcga tataatggaa
     6661 tatacaaata ataaaaaaaa tgaactgaga aagtctaagg aagttgaggc attgacatcc
     6721 cttatagaag tagaaaatcc agatacgatt ttacagtttg gtaggaaagc atctgaagga
     6781 gtagcaagag tttctgatag ccttttaaat acaataaaat taaatagaaa tgaagaaaat
     6841 tctaaaatgc tggttcattt aactaaaata atggataagt ttgatttaga cgattttcaa
     6901 gaaactaagg aaccaaattt cgttcaaaaa cttttcaaaa aagctaataa tgccattgaa
     6961 atgatgtttc aaaagtatga aacattggga ggagaagttg agaaaattca aattgagctt
     7021 gagcaatacg aaagagatat agcactttct aataagcaaa taggtgctat gttaaatgaa
     7081 aactttgagt tttacaatga acttcaaaaa tatattgttg ctggagaaat ggcaatagaa
     7141 gaaatggata atgaaatact acctttcttt aaacaaaaat cagagactag tggagaccaa
     7201 atggatgttg tcaattatca agaactatta aaagtttatg atatgttaaa tcaaagagtc
     7261 tatgatttaa ggatagcaga aaatatagct atccaaacaa tacctatgct tagaggtatg
     7321 caacataata attatggttt aattagaaaa ataaatagtg catttgtcgt aactttacca
     7381 gtatttaaac aatgtttatc tcaagctata ttacttaaaa agcaagaatt acaagcaaaa
     7441 tctctaaaag cacttgatga caaaactaat gaattattgc ttagaaatgc acaaaatgta
     7501 agtactcaaa gtgcacaaat agctagaatg gctggaacaa gttctgttca aatagaaaca
     7561 ttggaaaaaa tgtacaatac tatcaaatct ggtatagatg aaactatgag aatagaagaa
     7621 aacaatagag ctttaattaa ggataataca aagagattgg aagaattaaa tacgactata
     7681 atttataata agtagtatta tattaagggc taatttgtat aaagttggcc ctttctatgt
     7741 atatgaggag gctagacatt gaaatatttc gattatatct gcaaagatga tttggagaga
     7801 atttttttga aggagccaga agattttagt gctaagactg aaaaagatgt gttgaaatat
     7861 gcactgggcg cttttttata cgttccagct actcaatata atatgattta taagagtatt
     7921 ataggggatg ttaagggagt tagaccatta gctatttgtt tagaagatgc tgttggagta
     7981 aatggagagt tggaggctat agaaaatctt agattaattt taaaaaatat tagcaatgaa
     8041 agtataacta ataaagatgg aataccacta atttttgtaa gaataaaaga tgtggagcaa
     8101 ctgttgagaa ttaaagagat tataattaaa aatagtcatt ttataacagg aatattaatt
     8161 cctaaagcaa attcggagtt aattgaaaat tgtatagaag ctttagattc tatgaatctt
     8221 caagatatgt atgttattcc gatagtagag acaaaagaat ttatatataa tgaaaaaaaa
     8281 gaacttagtt ttactaattt gtacaatgct attttaagac ataagtcaag aatattaagc
     8341 attagagtag gcataactga tatattaggg atgtatggta taaggagaga taaaaacttt
     8401 tctatatata ataatttgat ttgttcttca tttatattag atataatcac atatcttcaa
     8461 agaccagagt tagatatacc tataagtggt ggagtatcag aattttttga tatgacgaat
     8521 aaggatataa gaaataagta tatagaagaa atattattgg ataaatatca tggattaatt
     8581 ggaaagaccg taattcatcc aatgcaaatt caaattgtac aagcgctatc tacagtatca
     8641 tatgaagatt ttactgatgc tttggatata ctggatagta cagacagtaa gtatggtgtg
     8701 agcaagggtg tattaggaga gagaatgaat gaaacaaatc cacatttttt atgggctaaa
     8761 aaaacattga ttctatctaa aatatatggt gtattaaata aaggagtaga ctatgaagaa
     8821 ttgcttaaat tttaattgtg agattgacat tattgataat ccattgaatt tagaactaaa
     8881 tgatttttta gacgtaggaa taagaaataa tagcaaaaga cgttttttgt ttatctcaaa
     8941 gaaactcggt aaacatttag catgtaagcc aagtgagatg gataatttgg gaaaattaat
     9001 ggctacaatt tataatgaaa aaaataagga agaatcaaat ggtactgtta tagcttttgc
     9061 tgaaactggt acagctgttg ggcatagttt ttttgattac ttatgtggag attatgaatt
     9121 tattcatact actagagagc agtttgacga gttagaaaat ctggaatttt tagaggaaca
     9181 ttcccatgca acagaccaga atttatactt tggtatgctt aacaacttta aatttggaga
     9241 tgagataata ttggttgatg atgagattac cacagccaat acatgcatga atataattag
     9301 gaaaattcaa agcatttatc ctaaaaagaa atacactata tgctcaatac tcaattgggt
     9361 aagtgatgaa aatttaagta aagttactcg tttagaaaga gagctagatt gtaagataga
     9421 ttttgtatac ttatttaaag gaaactttaa attttatata gatgaagata ttatttcaaa
     9481 ttacaacgat aattatagta ttactaaatt agagactatt tcaagtgata aagtaaattt
     9541 agtagtaaat catatttatg ttgatttaga agattatatt ggaaataaga aatatgtaaa
     9601 atatacagga agatttggaa ttaatagaag agaacaattt agattaaaag aaatcgttaa
     9661 gagagaaagt aaaaaaataa ctatggccag taaagaatct ttaaaaaatg aaaaagaaaa
     9721 gattctattt ttaggtactg aagaatttat gtatatacct atgctgtttg caaaagaaaa
     9781 agaaaatcaa tatgatatat actatcattc aacaacaaga agtccaatta ttaatattga
     9841 taagaataat tatcctatta aaagtaaatt tatgctaaag agtttttata atatagatat
     9901 tcaaaattat atttacaata tagataaaca taatttttct aagtgttatt tatttgtaga
     9961 gctgaataaa agtgaaaatt cttatctaga atttattgat ataattaaaa aaacaagtat
    10021 aaaagaatta actatagtat gttgtagcta aagtgtttta acaggataaa atatatcacg
    10081 attttaagat aggagttgag tcatatgaga gattttagca cgtattctaa agatgatgtt
    10141 atttttttat taaaagatat atcaaattta attgaagaag aaggaaataa agaaagagaa
    10201 ttaaaaatac aaaatggtag acattattca gaaatgatac caatagagta tgaagttagt
    10261 gaagattatc tgaatttata tcatgagaaa ctaagagaaa ataaagataa acttgcattt
    10321 tctataggag ttatgtgtga aaaaataatt aaaaaaaatg gaaatgatgt agtactagtt
    10381 tcacttgcta gagcaggaac acctattggt attttggcaa aaagatatat aagaagcaag
    10441 tataatataa gtttacctca ctatacaatc tctataataa gagataaggg aatagatatg
    10501 aatgcaattg aatatatagt aaagaatcat ccaagttcaa aaattcagtt tttagatgga
    10561 tggactggta agggaactat atcaaaagaa cttgaaaaag catgtgatga acttaatgtt
    10621 aaatttaaaa aatattttga ctcaacactt gctgtattag ctgaccctgc tggatattca
    10681 ggactttatg gaattagaga agatttctta ataccaagtg catgtttaaa ttcaaccgtt
    10741 tctggtttag taagtagaac tgtactaaga gaggatttaa ttggtaaaaa tgacttccat
    10801 ggagctaagt tttatagaca tttaaaagat aaagatgaat ctatgaacta tatcgaaact
    10861 atagaagaat gttttaaaaa tcaatttaag aatatatctg atgaagttga aaactgggaa
    10921 agtgacatta taacaagaga tggatacttt gatgttttaa atataaaaga aaagtataac
    10981 ataacagata ttaactttat caaaccaggt gtaggggaaa cgacaagggt tttacttaga
    11041 agagttccgt ataagattct tgttaaagac ttaaatgata aatctttaga ccatatattc
    11101 atattagcta aggagaaaaa tgttgaagtt gaacaaatgg atttaaaagc ctataaatgt
    11161 tgtggaataa taaaaaatat gaaagatatt taggaaatat attagacagg agaatattga
    11221 atgattgttt tttcagattt agacaggagt atcatttact ccaataaatt tttaaatgct
    11281 gatagtaaat atactaatat agaaatttat agggaaaaag aaatatctta tatatcttta
    11341 gatactataa atttaataaa gcaaatacaa tattatggta tgtttatacc tacaactact
    11401 agaacagtgg agcaatttaa gcgtatagaa tttaataaat atgggattta ttttccttgg
    11461 agtataactt caaatggagg agttatactt aaagataatg aaatattgaa atcatggagt
    11521 gaaaaaatag ataaactaaa atctgattat gagcccattg aaagtatgat tcataaattt
    11581 aaagactatt tgaatgtaga tggaataact aattttaaag ttgcagaaga tacatttttt
    11641 tatatagttg ttgatttatc aagatttaat ttggattcaa tcaaagaata tacaaatata
    11701 tttgaatcaa aaaattggaa gttttatgtg agtggcagaa aaatatattt tataccaaaa
    11761 gaaatttcaa aagaaaatgc tataaaatac ttgactaaag aattaggaat agaatatttt
    11821 tatgcagttg gagattctat tatggactat ggtatgctta atatctcaaa taaatcctat
    11881 gtattgaagc atggtgatat aaataaaaat gaaatagaaa attcatttat atcaagttct
    11941 ttcaatggaa tgagtggaac tgaagagata ctatctaaca ttttagacga gaactgccta
    12001 cttaatatat aatgactgat atataaataa taaactagaa aaatctgaaa taaattatag
    12061 taaagtttat catactaatt tatttcagat ttttatcaat ttatatttaa gtaagaactc
    12121 aatatgttaa aatgaaggtg gagtcattgc ccagactgag atacatacct catcatcata
    12181 attttcatac acatgtggta tagtactgtt aaaataaaaa ctatctcctt cctccaatag
    12241 atattcctta tttccaagtg ttatcttcat tttacctttt attatatagc cacattcttc
    12301 tccttcgtgt gtcacaaatt cactgttttt ttgtgtatga ccatctagcg taacaagtat
    12361 aaattctatt tttttcccag ataaattagg aacaagaagt tcataaaatt ttgtagaatc
    12421 attagttact attttttttc gattattttt ctttactact atatcagagt cctctcttcc
    12481 aatctcttca aaaaaataac ctatatttat atcaagagct tttgatattt tccacattgc
    12541 tacaatagat ggaacaacca tattacgctc aatttgactt attaagcctg tacttaaatc
    12601 tgaatttttt gctaaagtag agatacttat atttttagat tttctaagag atctaatttt
    12661 gcttccaaca tctatattca ttagatacaa accccctcag acatgattat aacatataat
    12721 ttataaagat taataaaata aaaaaaagtt ttacaataca agtatttttt atgctatatt
    12781 gaattttctg aaaagatgca ttataatagt attaatattc agtatattga atattgataa
    12841 cgatatttta atttaatgaa atggaggtaa ttaagatgaa gagttttgta agtcctgata
    12901 atatttgggt attatgggca gttgtaactg gatgggcagc atttagtatt tatttggaac
    12961 aaaagtacaa ttgggcatca aaagtgtcgg gagcaataat agctcttgta ggagcaatgt
    13021 tattatcaaa tctaaatatt ataccagtag agtctgttgt atatgaccaa gtatggggat
    13081 atgttgttcc tttagccata gctttattgt tatatcaatg taacattaaa aagatatgga
    13141 aagaaagtgg aagattgctc attatatttc ttgtaggttc tgtaggaact gtacttggag
    13201 ctatgattgg atttttagca ttaaaaaatg tagtaccaga tttaaatatt gttgcagcta
    13261 tgatgacagg ttcttatata ggtggaaatg taaactttgc tgctatgtca ggggcgtttg
    13321 atgcgccagg agaattagta tcagcaacag ttgtagcaga taatctttta atggcattat
    13381 atttctttgt cttaatagct ataccatcaa ttggattttt tagaaaacat tttaaacatc
    13441 cacatgtaga tgaaatggaa agtataggca taactgaagg agaaacagta gcagcaaatt
    13501 attggggaag aaaagaaata tctttaaaag atatagcaat agccattggt agcgccttta
    13561 taattgtcgc agtatctgta gaactatcaa cttggtttaa agctataata ccattatcaa
    13621 atcctttttt agcaatgtta aatactttat ttggaaatca atatctaata ataactactt
    13681 tgactatgtt gtttgccaca tttatgccta atttctttgg tgatataaaa ggagcacagg
    13741 aattgggtac atttttaata tatatattct ttgttgtagt aggtgttcct gcttcaataa
    13801 cattaataat tcaaaaatca ccattactac ttttatactg cggaataatg gtactaataa
    13861 acatgctagt tacatttata gttgcaaaaa tatttaagtt tagtttagaa gaagctattc
    13921 ttgcatctaa tgcaaatatt ggtggtccta caacagcagc agctatggca atatcaaaag
    13981 gatggtctaa gctagtaggg ccaatactta tagttggaac atttggatac atagtcggta
    14041 attattttgg tcttttagta ggaaatattt tgatataatt ttacgtgaaa aaaagttgtt
    14101 ttttagaaag atttttattt aattggaggt tgttatgatt ttcgatggac attcagatac
    14161 ttgggcagat gtaacaataa gaaggcttaa cggggaaaaa gatgtattta gaagaatcca
    14221 tcttgataat tttaaaagag caaatataga aggagctatt tttgtaatat ggatagaccc
    14281 accatatgat aaagagcctg aaaaaagagt taaacaaatt ataagctcta taaaagatga
    14341 aattgaagat atgaaagatg ttgtaaacat agtaaaaaaa tattcagata tagataaagg
    14401 tataagtgaa aacaaaataa atattttaat tggtcttgaa ggattaagcc atattggaag
    14461 aaatatagat ttactagatt atttttataa agaagttggt gcaagacatg catcattaac
    14521 ttggaatgaa cagaatgaac ttgctacagg atgtttaggg tatgaaaatc gtggtttaac
    14581 tgaaataggt aaaaaagcag tcaataaaat ggaaaaattg ggcatgatag tagatgtgtc
    14641 acatttaaat gaaaaatcat tttgggattt aatgtctgtt gcaagtaagc cagttatagc
    14701 ttctcattct aatgctagga gtttatgtga tgctaaaaga aatcttacag ataatcaatt
    14761 aaaagaaatt tcaaatacag gtggattagt tggaataaat agttttagag gatttactaa
    14821 tagtaatcct aagaagcaag atttaataca tttggttgac catatagact atatggtaaa
    14881 tcttataggt atagaacatg tatcttttgg ttttgatttt agtgattact tagggaaaga
    14941 gactataggt tcttttactg gaaatgctag tgtgccaagt gtagatggtc ttgaaagcac
    15001 acatacagca cagaacatag tagtagagct tgaaaataga ggttataaaa agaaagatat
    15061 agagttaatc tctagtggta atttttatag gctctttaaa gatatactaa aataatataa
    15121 taaagaactt aaatttataa aatagtatat ttatacaaat aatatctaca tatttaaatt
    15181 ggcgttcata atttattaaa ttaaatttta atgttactag aagtttcaat tctagtaaca
    15241 ttttttatgt taaaaacaga tatagatatg tattgacaat gataattatt attgataaac
    15301 tataaaagtg aaaataataa taattttcat tttaattaat aaaagatttt taaggaggga
    15361 gaatgaagaa aaggtattta gtcataggaa taataatttt gtcttatttt tctttgttta
    15421 taggagctga agatataaat ataatgcata tatttgcaaa aaatcagcat aaacttatga
    15481 tatttatcat gagtagaata ccaagattaa taagtatatt gattgctggt gtaggaatga
    15541 gcatagcagg tcttataatg caacagataa gtaagaataa atttgtatca cctacaacag
    15601 gagctacaat agatgcagca caatttggga tagtaatatg tatgctttta gtaccaactg
    15661 cctctatttt tacaaagaca ataatagcat ttgtattttc tcttgtaggg acttttacgt
    15721 ttatgaaaat aatagggaag ttacaattta aaaatataat ctttgtacca ttagtaggta
    15781 taatgtttgg aaatataata ggttctatga cggattttat cgcatataaa tacgatttga
    15841 gtcaaaatgt tagttcttgg atgcaaggtg acttttctat gatacttaaa ggaaattatg
    15901 agatattata tattacaata ccccttataa tattagcata tatttatgca aataaattta
    15961 ctgttgttgg tatgggtatg gattttgcga caaacttagg tcttagttac aaaagaattg
    16021 tcaatatagg tctgataata gtagcacttg ttacagtatg tgtagtcgtt acagctggta
    16081 atatcccttt tataggtcta atagttccca atatcgtatc cttgtacatg ggtgataata
    16141 taagagcaag catatggtat actggtttac ttggagcgat atttgtactt atatgtgata
    16201 tttttggaag aattattatt tatccatatg aaatatctat agggcttaca gttggagtta
    16261 taggaagtat actattcctt tatttaatat taaggaggaa tgtaaatgaa gcttagtctc
    16321 aatcaaaaaa tttactttgt aagtatatta ctattcatat caatagcact ttttataatt
    16381 tatggaatag acatgaatca tttagaatat gcattatcac aaagaatacc aaaaatattt
    16441 gctatggtat taggtggagg atgtatagca tttactactg tagtgtttca aacaattaca
    16501 aacaatcaaa tattaacacc aagtgtacta ggtttagatt cattgtatgt aatgattcaa
    16561 actataatag tattcatatt tggttcaagc agtagtctga ttataaatga aaattataat
    16621 tttattatta atgtagctat gatgattgga gcttcattgt tattatataa gacgttattt
    16681 gaaaaaaata aaaataatat tttcttttta atcctagtag gaatgatatt tggaacctta
    16741 tttaaaagtg caacaacatt tattcaggta atgatagacc cgaatgaatt tttagcttta
    16801 caaactagta taatggcaag tttaaacaat ataaacacta atgtattgtt aatagcattt
    16861 ataatagtta ttgccataat accttttata tatgatgaaa taaagtattt agatgtattg
    16921 tcgttaggta aagaacaagc aattaattta ggtgtagatt ttgataaagc agttaaaaaa
    16981 atgataatat taatagcaat tttagtctca atatcaacag cacttattgg acctatgaca
    17041 tttttaggac ttttacttgc taatataaca agagaaattt ttaaaacata taagcataca
    17101 tacttgattt cgggttctat gttgataggg atgattacat tgataatagg tcaatttttt
    17161 atacaacatg tatttaagtt tgatacaact ttaagtgtag taataaattt tataggagga
    17221 atttacttta ttcaattact attaaagggg gcaaatagat gatagagata aaaaatatat
    17281 ttaaaagata taagaataaa aatgttgtag atgatgtctc ttttagtata gaaaagggaa
    17341 agataacatc ttttatagga ccaaatggag ctggaaaaag tacagtacta tcaatagtta
    17401 caagacttat gggaggtgat ggtggagaag taataataga aggaaaaagc ttgactaatt
    17461 atagcaataa agaattagca aaaaaaatag ctattcttaa acaatctaac aatataactt
    17521 taaagcttac aataaaagaa ttagttggat ttggaagatt tccatatagt gaaggtaatc
    17581 taactaaaga ggatgaaaat tatattgatg aagcaataga atatatgaag ttgactgata
    17641 tacaacataa gtatttagat gaattgagtg gaggtcaaag acaaagagct tatatagcaa
    17701 tggtaatagc tcaagataca gagtacatat tacttgatga accactgaac aatttggata
    17761 tgaaccattc cgtacagatg atgaaggtac ttagaggtct ttgtgatgaa ttagataaga
    17821 ctatagtatt agtaatgcat gatataaatt ttgcatcatg ttattcagat aatatagttg
    17881 cattaaaaaa tggaaaagtt gaaaaagtag gtcgtacaga tgagatagta aatgaaaaag
    17941 ttttagaaga tatttatgag atgaatttta acattaagaa tataaatgga aatcgaatct
    18001 gtatttattt ttaaaatatt gtatatgaaa ataaacttgc tatatgattt tttataaagt
    18061 ttttaaatta aaataaagat taataggaga gaggaaaata ttgatatgaa taaaaaagca
    18121 gcaatagtag cagcagtagc tattatagga ttagtgacag tgtttgcatt aggtggaagt
    18181 aaaaagaatg aatcaaaaac atcagaaaat tcaaataata ctatgaagat aactcataac
    18241 ttgggagaaa cagatgtaaa attaaatcct aaaaaagtag tagtatttga ttattcggct
    18301 ttagatacaa tggatgcatt aggtgtagct gagaatttag taggacttcc aaaagcaagt
    18361 ttaccagcat ccttagaaaa atataaggat gacaagtatg cagatttagg aggattaaaa
    18421 gaaccagatt tagaaggtat aaagtcggca aatccagatt taattataat aaatggaaga
    18481 caagaagatt tttatgagca attgtcaaaa atagcaccaa caataagtac aagtaaagat
    18541 gataagaagt acttagaatc tgttaaaaat aatatagaca aaatagcaaa aatatttgga
    18601 gtagaagaaa aagcaaatca agaatttagt aaaattgaaa agaaaataga aactttgaat
    18661 aaaaaagtaa cagataaaaa tttaaatgct ttaactataa tggtaaatga aggtaattta
    18721 agtgtatttg gtgaagaatc aagatttagt atattataca atagttttgg atttgaaaat
    18781 aaagataaaa atataaaaga atcaagtcat ggacaaaata ttacttttga atatatagct
    18841 aaacaaaatc cagaagttat gttcgttata gatagaggaa tagctactgg cagtgatgta
    18901 aaagaaagct caactgcaaa atctgttttg aacaatgata taataaaatc tatggatgca
    18961 tataagaatg ataatataat atatttagat tcaccaacat ggtatgtaaa tgatggtggt
    19021 ctaacatctt taaataaaat gatagatgat gcttcaaaag cggtaaatta ataatccgat
    19081 ataattaatc aaaatgtatt ttttataaaa tgattatcaa ataaactagg agtttttaag
    19141 aactcctagt ttattttttg cataaaaaag atatagttta aacaataata ttatctataa
    19201 taagataaat ctattcaaaa tctaataaaa tatgaagtta tactagacaa aaagaat
//
LOCUS       DAEMHW010000061        19224 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEMHW010000061 DAEMHW010000000
VERSION     DAEMHW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19224
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.061"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            163..684
                     /locus_tag="KPK67_001828"
     CDS             163..684
                     /locus_tag="KPK67_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2458797.1"
                     /translation="MDIAKLILNPARLRVLQYIRLHSSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            718..1494
                     /locus_tag="KPK67_001829"
     CDS             718..1494
                     /locus_tag="KPK67_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2458798.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1837..2790
                     /locus_tag="KPK67_001830"
     CDS             1837..2790
                     /locus_tag="KPK67_001830"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBG2458799.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2808..3827
                     /locus_tag="KPK67_001831"
     CDS             2808..3827
                     /locus_tag="KPK67_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBG2458800.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3858..4109
                     /locus_tag="KPK67_001832"
     CDS             3858..4109
                     /locus_tag="KPK67_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458801.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4391..6196
                     /gene="lepA"
                     /locus_tag="KPK67_001833"
     CDS             4391..6196
                     /gene="lepA"
                     /locus_tag="KPK67_001833"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBG2458802.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6345..7682
                     /locus_tag="KPK67_001834"
     CDS             6345..7682
                     /locus_tag="KPK67_001834"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBG2458803.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVGLFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            7915..9228
                     /locus_tag="KPK67_001835"
     CDS             7915..9228
                     /locus_tag="KPK67_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2458804.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMTNVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9400..10575
                     /locus_tag="KPK67_001836"
     CDS             9400..10575
                     /locus_tag="KPK67_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBG2458805.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10678..11697
                     /gene="hrcA"
                     /locus_tag="KPK67_001837"
     CDS             10678..11697
                     /gene="hrcA"
                     /locus_tag="KPK67_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBG2458806.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKRELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11727..12347
                     /gene="grpE"
                     /locus_tag="KPK67_001838"
     CDS             11727..12347
                     /gene="grpE"
                     /locus_tag="KPK67_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBG2458807.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12439..14286
                     /gene="dnaK"
                     /locus_tag="KPK67_001839"
     CDS             12439..14286
                     /gene="dnaK"
                     /locus_tag="KPK67_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBG2458808.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14497..15633
                     /gene="dnaJ"
                     /locus_tag="KPK67_001840"
     CDS             14497..15633
                     /gene="dnaJ"
                     /locus_tag="KPK67_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBG2458809.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGFGGFEDMFGDIFGDMFG
                     GSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGTGAKPGT
                     SKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTGNTRKVK
                     TIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGNDIYFEMP
                     ITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGDQYVKVV
                     VEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16599..17534
                     /locus_tag="KPK67_001841"
     CDS             16599..17534
                     /locus_tag="KPK67_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG2458810.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17570..18943
                     /locus_tag="KPK67_001842"
     CDS             17570..18943
                     /locus_tag="KPK67_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2458811.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
BASE COUNT         7638 a         2116 c         3500 g         5970 t
ORIGIN      
        1 taactaatat gttagaaggt tatatgtaat taatgtcagt gtttatatag aatagcattt
       61 gtagcattta ttatagttgt aattaccatt tatatcatag acttgaatag tctaaaaaaa
      121 tagactataa tatttttata gactaaagaa agggctgata agatggacat agcaaaacta
      181 attttaaatc ctgcaaggct tcgtgtttta caatatattc gacttcatag cagtgttcgc
      241 acatctgata ttgtaaagta tttaaatgat attcctcgtg caacagttta tcaccatgta
      301 aaaattttag aagagaacaa tatgattgaa gtaattaaag aaaatcgtgt gcgtggtact
      361 attgaaaagg tttatgcact aaaggaatat acaacatcta tggaaggaga gaccttcgtt
      421 gcactttcta cagcatttta tgtaggtctg atgcaggaaa tgaatgaata ctttagcaga
      481 aaagagcaag accataaaaa ggataatgta tttttctctt cagcattact atatatgagt
      541 gataatgaat atgaaaatct tcttaaaagt atagctgatt tattgaaacc atatatagaa
      601 caaaaaccta aatcaaatct aaaacttaga aaactttcaa ttatatcttc tccacctgta
      661 gctgataatg ataaaacaaa ataacatgca acccattttt ataaaaggag gttatatatg
      721 aaatttcatg agtttggaaa taaaaacaga ccacatatta tgttgattca tgggggaggg
      781 aatgcatggt ggaactattt gcgacaagca gaagttttgt ctaacaaata tcatgtgata
      841 cttcctacat tggatggtca tggagaagaa tatgaaacaa catacatttc tacagaggat
      901 acagcagata agttgatatc atatatagat gaaaattgca gtggacatct tttcgctttg
      961 tgtggtgtat cacttggtgg tcaaattgtc atggaacttt tatctcgaaa acctaatatt
     1021 actaaaaaag caattattga tggtagtatt tgctacccaa gacctcttat ggcaaggttt
     1081 tgtattgctg gtattcgatt ttttggagga cttttgttta gtgagaaggc ttgtcgcttt
     1141 caaattgcta tgatgccaaa attgttacct gaaaatatgc aatatccaga tgaaataaaa
     1201 gcatattata tgacagatat gccacatgta agaaaagaaa cattctataa tatgtatcgt
     1261 acttacatga tgaactatac acttaaagag aatgttaaaa aaaccacagc acaagtaatg
     1321 tactggtatg gagaaaaaga aatgaaatgt gttaaaaaat ctgcaaaaat gtttcagtct
     1381 tatgtgccct cttgtgagat ttatgaagct aagagctata atcatggata tttatcactt
     1441 tatcttccaa atgaatggtt ggaaattgca gaaccatttt tccaaaagga ataaaggtgt
     1501 taggtgatta ttgtataatt attgctgttt tttaatatag taaataaatc aggtataaat
     1561 ataaaaaaat tattttagtt aatatattag tgaaagctta ataggaaaat tataaataga
     1621 ttaaaggtag cgcctatata aatagttgct acctttttct gtttgtaaat aaatgataga
     1681 taagaaggtt tactgacaaa tcactgacta aacccaattt tcttacattg aaagccttaa
     1741 ttttacttgt tttaaataaa tttacaattt cttgttataa aatactaaat tttgttaaca
     1801 attataatta gcatttaatt tatactggag gtttatatga ttagtgtaag aacagattta
     1861 gctttagaag ctagtgaaat gtgtgaaaag tctcaagaag gaagtagtat tcctggtgta
     1921 aaaatagaaa ctaaagaatt agaaaattgc attgtaacta aagttgaagt aatagacagg
     1981 caaggcgcag agataatgaa taaagatata ggaaagtata taactctgga aagtaatctt
     2041 atgaagtttg atgatgatga gtcaagagaa gaaatgataa gttatttaaa agaggagtta
     2101 gtagatatat ttggccaaga caaaaataaa aaaaccctgg taattggtct tggaaataga
     2161 aatattactt ctgatgcgtt aggaccaaag agtgtatcaa aaacacttgt aactagacat
     2221 ctttttaaaa attataataa agactatgat gatgatttca ctgaggtatc agctttaagc
     2281 ccaggagtta tgggtgttac tggaatagaa actagtgaaa tagtaaaatc attggttgag
     2341 aaggttaagc cagatagagt agtagcaata gatgcattgg cttcaagaaa aatggagaga
     2401 gtaaactcta ctatacagat atcaacagca ggaatatcac ctggtggagg tgttggcaat
     2461 actagaaaat ctctaactaa agaaacttta ggcgtggatg ttattgcaat aggcgttcca
     2521 acggtggttg atgcagcaac tcttacaata gatgtcttag atatggcaat agacaattta
     2581 atagctcaat cagaagaaac agaaagtttt tatgaaatgt tgaaaaaact taaagaagaa
     2641 gaaaaatatc atctaataaa agattcgtta gacccatatg acaagaactt aatagtaaca
     2701 ccaaaggata tagatgatac aatcgaaaat ctatctataa taataagtga aggcttaaat
     2761 aggtctttac atccaggtag attggtataa aataaggagg agcaattatg tttaaaaaat
     2821 gcattaaagt agtaaccttg acttttatat tagtatgcat tttaccagga aaatcattag
     2881 ctttgaatca agatgatttt ttaaagtttt tagtaaattc atcttatcca gaagctaaag
     2941 tcgaaggaaa tgatacagaa aataaaaaga ataataaaaa taaagaaaca agtaaggaaa
     3001 ataaggaaga aagtaaagaa ggaaacacta aatctaaaga tgcaagtaaa gtcgataata
     3061 aaaaagagag tgaaaaagaa tatataaaac tttatgttgg taaagaaaat gttccagata
     3121 ttgagtcaaa aaattcagac acaactgaaa ctaacacaac atctagttca gattataagg
     3181 atgatttaag agtaaccaaa gaaaatccaa gaattttgat atatcatact catggatgtg
     3241 agacttattc aaattcacct gatggaaatt atcattcaag agataagaaa aattcagtaa
     3301 tggaagtagg aagtgcatta actagtgctt tagatagcaa aggatggggt gtagttcata
     3361 ctactaaata ccatgactat ccatcttata ataactctta tgcaagtagc ttaaaaacaa
     3421 tacaaagtat attacctaaa tataactcag tggatatagc gatagactta catagagatg
     3481 ctagggattt aactaatcca gccactaagg aaaaagacca tttaaaatat acaactatga
     3541 taaatggaga gagagtatca aagttcttct ttgttgtagg aggtaaaaat acaaatagaa
     3601 aacaacttag agctttagca gaagatataa ctgcttttgc tgagaaaaag tatcctggtc
     3661 tagtatctcc aatagtagaa aaagactatg ctagattcaa tcagtttgca gttaagaatc
     3721 atatgttagt agaaataggg aataacgcaa caagtgtaga agaatctaag gctactacaa
     3781 agtatttagc cgagattttg gatgaatatt ttaaacaaaa aaattagagc tttaagttat
     3841 aattaggagc atgctaaatg agccgattag aaaaaaatat agaaagtaaa agtaaactaa
     3901 gaaaatacaa gtatattatg agaatcttat ttttgattac tatgattata tgtacatcag
     3961 tttgtgtatt tatagtggac agtaatgcaa aaagtatgct aggaatgaaa caatatgatt
     4021 ttccaagtat taaatttgat ataacaaaat ttgattttga cataccaaat tttgatatac
     4081 atgaaatcat aaaaaaattt aataagtagt aataagtgaa taaggtttat tgattaaaat
     4141 aagccttatt ttagttatat atttttaatg tataaaataa aaattcatta actgtatttt
     4201 ataggattta ctttaagttt acgatattat ataatattta gtgttagact tgataatcca
     4261 catataatgt ggataatttt gtttagtgcc aattagttta tattcaaaaa ttttaaattt
     4321 ggtatataaa gagtttttag ataatgtaaa ataataacta taagaataat caagaggagg
     4381 gaattttaag gtggacaata aacaaagtag aactagaaat tttagtataa ttgcacatat
     4441 agaccatgga aagtctacct tagctgatag attaatacaa caaactgggc ttgttagtga
     4501 aagagacatg aaaagtcaat tacttgacaa tatggacctt gaaagagaaa gaggaatcac
     4561 tataaagctt caaaacataa gattaatgta taaagcgaag gatggaaatg aatattattt
     4621 aaatcttata gatacacctg gacatgtaga ctttaactat gaggtatcaa gaagtttagc
     4681 tgcatgtgaa ggagcactat tagtagttga tgcagctcaa ggtgttgagg cacaaacttt
     4741 agccaatgtt tatcttgcta tagaccaaga tttagaaata ttaccaataa taaataaaat
     4801 agatttacca agtgcaagac cagaggaagt aaaaaatgaa atagaagatt taatagggct
     4861 tgattcaagt gaagctcctc ttatttcagc taaaactggt ttaaacatag aagatgtttt
     4921 agaagatatt gtaaaaaatg ttcctccacc taaaggagat aatgaagctc cattaaaggc
     4981 attaatattc gattcttact atgatgctta caaaggtgtt gtagcttatg ttagagtatt
     5041 tgaaggaact gttaaaaagg gtatgacaat aaaaatgatg aataccaata aaaagtttga
     5101 ggtaactgaa gttggtgtta tggctccagg tcaaactgaa cttagtgaat tatcagcagg
     5161 tgatgttggt tatattgctg ctagtataaa agatataaga agttgtcgtg ttggtgatac
     5221 aataacagat tcaaataatc ctacagaaga acctttacca ggatacaaaa aagctactcc
     5281 aatggtatat tgtggtatat atccaggaga aggagaaaag tatgagaatg ttagagatgc
     5341 tcttgaaaag cttcaagtaa atgatgctgc acttgaatat gaagctgaga cttcagcagc
     5401 actaggattt ggttttagat gtggtttctt aggtctttta catatggaga taatgcaaga
     5461 aagacttgag agagaattta accttgatat aattactaca gctccatctg ttatatatag
     5521 agttacaaaa atggatggag aggttgtaat gatacaaaat cctgcaaact taccagaacc
     5581 atctgagata aagatgatag aagagcctat agtaaaagga gatataatag ttcctaaaga
     5641 ctatgtgggt gttgttatgg agctttgcca ggaaagacgt ggaaatatgt taaatatgga
     5701 atatatagat gagagaagag taatgcttca ctatgacctt cctctcaatg aagttgttta
     5761 cgatttcttt gatgctttaa aatcaagaac tagaggttat ggttctcttg attatgaggt
     5821 taaaggttat gtagcatcta cacttgtaaa acttgatata ttaatcaata aagagcaagt
     5881 ggatgcactt agttttattg tccatgagac acgtgctttc cctagaggaa aggcaatgtg
     5941 tgagaaatta aaaggagaaa ttccaagaca tcagtttgct ataccaatac aagctgctgt
     6001 tggtaataag gttattgcaa gagaaacaat aagcgctcta agaaaagatg ttcttgctaa
     6061 atgttatgga ggagatatat ctcgtaagaa gaaacttctt gaaaaacaaa aagaaggtaa
     6121 gaagcgtatg agacaaattg gttctgttga agttcctcaa aaagcattta tgtctgtgct
     6181 taaattagat gagtaaatcc ttataaattt agataaagat atttaaaagc tagagtgatt
     6241 ttagaattat ttctaaatta gtctagcttt tttatggtat attttaagta aactcataga
     6301 gaatataagt aagaatatta aagaagaata aaaaggtgat atttatgatt aatatagaaa
     6361 ttcctaaaaa agtagactat ataattaaag aattagaaaa gaatggttat gaagcttata
     6421 tagttggagg atgtgttaga gattgtttat tagaaagagt tccaaacgat tgggatataa
     6481 caactagtgc tagaccagaa gtggtagtag aattatttga aaaaacaatt ccaactggta
     6541 tacaacatgg gactgtaaca gttatgattg aacatgaacc atttgaagtg acgacttata
     6601 gaattgatgg aaattatagt gatggtagac atccagattc aatagaattt acaaataaca
     6661 tagttaagga cttgtcaaga agagatttta ctatcaattc aattgcatac aattcaaaga
     6721 caggattagt cgacccattt aatggctatg aagatataca aaataagtat atacgttgtg
     6781 ttggaaatcc agtagataga tttgaagaag atgcacttag aatgcttaga gcagttagat
     6841 tttcagcaca actcaatttt aaaatagctg aaggtactaa gcaaagtatt cataaaaaag
     6901 cagatttgat aaaaaatgtt tctattgaaa gaatccagac agagtttaat aaaatattag
     6961 tatctgattc atctaaatta aacttattaa aatcaacagg tttgttgaaa tttataattc
     7021 ctgaaatatg tgaattagaa gatgttactc aacacaatcc atatcacatc tatgatgtac
     7081 aaaaacatac tttaatagct acagaagtaa ttgaagatga actgtattta aaacttacaa
     7141 tgttgtttca tgatttaggt aaaaaagtaa ctaaaacaac tgataaaaat ggtgttgacc
     7201 atttttatac tcatagtaga gaatctgtaa agattgcaaa gaagattttg aaaagactta
     7261 agtatgataa ttatactatt aataaagttt taatattaat acaatatcat gattatagaa
     7321 tagaacctaa aaggaagata attaaaaagt tattaaataa acttgaagat gttggattat
     7381 ttgaagattt aataaaagtt aattgggctg atacacttgc aaaaaatcca aagtatgcaa
     7441 aacaaaagat attaaatctt attgagtgtg aaaaagaatt taaacatata ataaatcaaa
     7501 aggagtgctt taatcttaaa gatttagcta ttaatggaaa agatttgata tctataggta
     7561 taaaaccagg taaagatata ggatatattt taaataagat gcttgaaata gtcataaata
     7621 atccagagtt aaatgaaaaa gaaatactaa aagaaaaagc attaaatata tatacatttt
     7681 agattcaaat ataaaaaatt taattacttt aaaaaacata aaataaaata cttatttaga
     7741 taaaataaat aatttttaga gaatatttta gaagttacga ttgtatgatt tttaaatgca
     7801 taggcattta ctctaggact atagactaat ataaaaaaaa actttgggaa tactaaaacc
     7861 ataaatttat aaaattaata ttttatatac tgtaaaacag tgaggaggga cagtatgcat
     7921 actcaactac aaaagactat tggtctttca gcagctcttt ctactgttgt aggtatggtt
     7981 ataggttcgg gagttttttt taagccacaa gcaatataca ctacaactaa tggagctcca
     8041 ggtctaggta ttatagcgtg gcttttaggt ggttttataa caataactgc tgggcttaca
     8101 gctacagaaa tttcagctgc tataccaaaa actggtggta tgatgattta tatcgaagaa
     8161 atatatggag aaaagttagg ttttttaact ggatggatgc aaactgtatt atttttccca
     8221 ggtacatcag cggcattagg agtaatattt gctcaacaag catctgaact tttaggaatg
     8281 agcccaaata atatgacaaa tgtacttcct atagcaattg gagttatact atttttagca
     8341 ttattaaata taataggttc atccttagga ggaaaagttc agacagtagc cactattgga
     8401 aagatgatac ctttaatttt aataatagtt tttggattta ttaaaggtca aagtagtgaa
     8461 gtattaaatc catttgtagg agatggtgta aatgcatcaa atgcacttgg acaagtatta
     8521 atagctactt tgtttgcata tgatggttgg ataaatgttg gagctatatc aggagaaatg
     8581 aagtctccag agaaagattt accaagagca atagttggtg gtttatcatt agttatggca
     8641 gtttatatta taataaatat agcttatctt tgggtagtac cagcaagtga attagcaaca
     8701 gttacttccc cagctacatt agtagcaaca agattgtttg gaaatatagg tggaaaagta
     8761 ataactgtgg gtatattaat atcagtattt gggacattga atggatattt attaacaggt
     8821 tcaaggatac cttatacatt agctgaaatg ggaactttac cagcatctaa aactctttta
     8881 aaagtaaatt ctggaggttc tcctgtaaat tcaatactac ttataactgt gttagcctgt
     8941 gtatatgcat tatctggtca atttaatctt ttgacagact taacaatatt ttcaatatgg
     9001 gtattttatg tattgacttt tataggtgtt atgagactta gaagacaaaa gccagattta
     9061 cacagaccat ataaagtccc tttatatcca ataataccaa ttatagctat attaggtgga
     9121 ctgtttgtaa taataaatca aatattgact tcaacagtca tatctttagg tggaatattt
     9181 ataacactat taggtttacc agtttattat tatatgaaaa aaagataact acttaattta
     9241 agttagataa atttgggtaa aggtgaaaat taacttttat ccaaattttt tatactgtta
     9301 tacaatataa gtattatata ttgcttgatg tgctattttg tttgatttat aatttagtaa
     9361 ttgatgtttt aagataattt aagaaatgag gagtgaaaga tgctaggact ttatgtacat
     9421 attccatttt gtgtgaaaaa atgtaagtat tgtgatttta attcttataa aatggacata
     9481 gattcaaaaa aaagatatat agaagactta aaaatagaaa tggaattata tagtaataaa
     9541 ttatataaag ataataaata taaaaataaa gaatgctgta gtctaaataa aaatgacaaa
     9601 ataacgagta tatttgtagg gggaggtaca cctagtatac taacctctga tgaaataaga
     9661 gaagtattta taagtataaa agaaatgttt gatatagatg agaatgcaga aataactata
     9721 gagtgtaatc ctgggacatt aacattagaa aaattaaaaa ctatgaaaga gattggcata
     9781 aatagactta gtatagggct acaagctatt caagaaaagc atttgaactt tataggaaga
     9841 atacatactt atgaagaatt tgagaaaaac tataaagatg cattaagtgt aggttttaaa
     9901 aatattaata tagatttaat gtacagtctt ccaaatcaaa ctctatgtga ttggaaggaa
     9961 actcttgaaa aagttgtaca cttaaatcca acacatatat cagcttattc ccttatacta
    10021 gaagaaggaa ctgaactata caatatgtat gaaagtaata aatttgaact tatagatgaa
    10081 aatgtagata tagaaatgta tgagtataca ataaactact taaagtcaaa aggatataat
    10141 caatatgaaa tatcaaacta ctcaaaagaa ggctataatt gtgagcataa tattttatat
    10201 tgggaatgtg aacattatat aggaattgga gcaggagctt ctggctatat taatgagaac
    10261 agatacaata atgtagaatc tttagaagat tatcatttaa gtttggtaaa aagagaaaaa
    10321 ccaatacaag aaaatgaaat tctttctgag aaagatatga tagaagaaaa gatatttatg
    10381 gggcttagaa tgaataaagg cataaaattt gaagatttta aaaaaaaatt tggaatagat
    10441 tttagagaga agtataataa gcaaatagaa atgctattag ctagaaagct tataaatcag
    10501 agctttgaag gtatacaatt aactcaaaaa ggaagagaaa tatctaatag tgtatttata
    10561 gaatttatgg aataattttt aaggtgttga caaaataaat ttataatggt atataatata
    10621 tataatcttt ttagcactcg aacttagtga gtgctaacag ctaggaggta taataatatg
    10681 gaattaaatg aaagaaaatt aaatatcctc aaagctatag ttaaagatta tatagaaaca
    10741 gctgaagcta taggttctag aacaatatct aaaagacatg atttaggtgt tagtgctgct
    10801 accataagaa atgaaatggc tgaccttgaa gaattagggt atttaattca gcctcatact
    10861 tctgcgggta gagttccatc tgaaaaaggc tataagttat atgtaaactc tcttatgagt
    10921 aaaagagaat tggatgataa tgataagatt cttattgaac aatgtatgaa tcataatata
    10981 aatcatataa aagaattgat tcatgaaact tctaagctat tatcgcaact aacaaactat
    11041 acaactgtag cagttactaa gagtttaata aatcaaagtg ttataaagca catacagttg
    11101 gttgctatga atgacaataa tattttactt atagttgtaa cagataaagg ggacttaaaa
    11161 aaagctaacc ttacaactaa tgtatattta gatcaatcta agttaaattt gatttctgat
    11221 aatcttacaa ggaagctctt aggtaaaagt ataacagatt tggatgataa tctaattgct
    11281 tttattaaat acgaaataag tgagtattct gggcttatag atgagctttt aaatgctttg
    11341 aactcaaata tgaaagaaga agatttttct ctatcattaa atggagctac taacatattt
    11401 agttatccag aatttaatga tgtattaaaa gcaaagtcat ttttaaatat gttggaaaaa
    11461 aaggaaacaa tagctgacat aattaaatca aagggaatac aaaaagacaa tctaaatata
    11521 ataattggta gtgataatga ttgtgaactg gcacaggatt gtagtatagt tacagcaact
    11581 tataatgtag atagagactt ggttgggcga ataagcttta taggccctac cagaatggat
    11641 tatgctagaa tttactctat aataaattat atgagtttat taattaatag aaagtaaaga
    11701 aattttaaat tgcgaggtgt aaagtcttgg ataaaaaaaa tgagcaacaa gaagtaagag
    11761 aagaaaatga tacttctatt aaccaagaat cggaaactca agtagagtta gaagaggaag
    11821 ttgttaatga agaatgtgaa acatcaagtg aaaaaactga tgaaaaagaa gttgatgatg
    11881 aaaatgtaac agatataaat tctaagttag cagaaaaaaa attgcaggat gagttggatg
    11941 aattaaatga taaatatcaa agacttcaag ccgaatatgc aaactacaga agaagaactc
    12001 aacaagagaa agagacaata ggcgtatttg caaatgaaaa aataataact gaattgatac
    12061 cagttataga tagtatggaa agagccttag atgcatgtga agataaggaa gatactatgt
    12121 ataagggtat aagccttgta cataaacaat taatagacac tttagtaaaa tttggagtag
    12181 aagaaataga agcagaatct aaggaatttg accctaatct acatctagca gttatgcaag
    12241 aaagtgtaga tggagttgaa gcaaatcaaa tagtaatggt acttcaaaaa ggatataagc
    12301 taggaactaa agttgtaaga ccttcaatgg ttaaagtttc ttgctaagta taaattaaag
    12361 gttaaattat ttaacctaat ataaaatatt aaaataagta aaacttaatt atataattac
    12421 tcgaggagga ttttagtcat gggaaaaata ataggaatag atttaggaac aacaaattca
    12481 tgtgttgcag tgttagaagg tggagaagca caaataatag caaatagtga aggtatgaga
    12541 actactccat cagtagtagc atttacaaaa gatggtgaaa gaatagttgg agaacctgca
    12601 aaaagacaag cagttacaaa tgcagataaa acaataactt ctataaaaac tcatatggga
    12661 actgattata aagtaaatat agatggaaaa tcatatactc cacaagaaat atcagcaata
    12721 atattacaaa aattaaaatc agatgcagaa agttacttag gacaaacagt aacagaagca
    12781 gttataacag taccagctta ctttacagat gctcaaagac aagcaactaa agatgctggt
    12841 agaatagcag ggcttgatgt taagagaata ataaatgagc caacagcagc agctcttgct
    12901 tatggtatgg ataaattaga ccaagaaaag aaaatattag tatttgactt aggtggagga
    12961 acttttgacg tatctatact tgaaatagga gatggaactt ttgaagtttt agctacagct
    13021 ggtaacaata gattaggtgg agatgacttt gaccaaatag taatagatta tttagcagag
    13081 gaatttaaaa aagctgaagg tgtagattta agaaatgata aaatggctct tcaaagatta
    13141 aaagaagcag cagaaaaggc taagaaagaa ttatcatcaa caatgagttc aaacataaac
    13201 ttacctttca taactgctac agcagaagga ccaaaacact taaatataga tttatcaaga
    13261 gctaaatttg aagaattaac tagaggttta gttgaaaaaa ctatggaacc aactaagaca
    13321 gctttacaag atgcaggact ttctacaggt gatatagatg atgtattatt agtaggtggt
    13381 tctacaagaa taccagcagt acaagaagct gttaagaagt ttataggaaa agaacctcat
    13441 aaaggtataa acccagatga gtgtgttgca gcaggtgctt ctatacaagc tggagtttta
    13501 gcaggggatg ttaaagactt attattacta gatgttactc cattatctct tggtatagaa
    13561 actatgggta atgtaatgac taagataata gaaagaaata ctacaatacc aactaagaag
    13621 tctcaaatat tctcaactgc tgctgataac caaacagctg ttgatataca tgttcttcaa
    13681 ggtgaaagaa gtatggctta tgacaataca actttaggaa gattccaatt aacagatata
    13741 ccaccagcac aaagaggaat acctcaaata gaagttactt ttgatataga tgcaaatggt
    13801 atagttaatg tttcagctaa agatttaggt acaggaaaag agcaaaaaat aactataaca
    13861 tcaaacacta atttatcaga agcagaaata gagcaaaaga taaaagaagc agaaatgaat
    13921 gcagaagctg acaaacaaaa gaaagaaaaa atagaagcat tcaatcaagc tgaatctact
    13981 atatatcaaa ctgaaaaaac tttaaatgaa cttggagata aaataagttc aggtgaaaaa
    14041 gaggatatag aaaaagctat agcagactta aaagctgtaa aagataatca agatgctaca
    14101 gcagaagaac ttaagaaagc tacagatgaa gtaatgacta agttccaaaa agtatctcaa
    14161 gaaatgtacc aaaaagcagc tcaagaacaa caagcggcac aaggtgcaga acaagcacaa
    14221 gacaatggac ctaaagatga caatgtagta gatgctgact ttaaagaagt agatgaagat
    14281 aaataagcta tataatatgt aacactaaat gataattgga acatatatga tttgtgaaaa
    14341 tcatatagat gttaaagata tatggacaat acacatcata aagaatataa tatatcatag
    14401 attatataac ttaaagtaga atataagaat taatgatagc ttgtagttta ttattctaca
    14461 agctatcgtt ttgtaaaatg aaggtggtgt caatttttga gtacaaaaag ggattattat
    14521 gaagtcttag gtatcagtaa aggtgcagaa gctcaagaga taaaaaaagc ttatagaaaa
    14581 ttagcaatga agtaccatcc agatagaaac cctggagata aagaggcaga agaaaaattt
    14641 aaagagataa atgaagctta tgaagttcta tctgatgata caaagagaaa aacttatgac
    14701 cagtttggac atgatgggct aaatggtcaa ggcggttttg gcggtcaagg ctttggtggt
    14761 tttgaagata tgttcggaga tatatttggt gacatgtttg gaggaagctt cggaggtgga
    14821 agagcaagaa gaagagggcc acaacgtggt gcagatataa gacagagtgt tactatatct
    14881 tttgaagaag ctgcatttgg taaaaagatg tctataaaag taaatagaag tgaagaatgt
    14941 gaagaatgta atggaactgg agcaaaacca ggaacatcaa agaaaacttg ttcaacttgt
    15001 aatggaactg gtcaagttag aactgttcaa agaactccat ttggtaatat agcaagttca
    15061 agaccatgta gtgcttgtaa tggaactgga gaagtgattg aatctccatg tagtaaatgt
    15121 catggaactg gaaatacaag aaaagttaag actatagaag tagatatacc tgctggtata
    15181 gatgatggtc aaatgataaa actttctggt caaggtgaag taggagaaaa aggtgctcca
    15241 agaggagacc tatatattgt agttaatgtt aagtcacatc cactatttac tagagatgga
    15301 aatgatattt attttgaaat gccaattaca tttgttcaag caactttagg tgatgagata
    15361 gaagtaccta ctttggatgg aaaagttaaa tatagtgtgc cagaggggac acaaactgga
    15421 actgtattta gacttaaaga gaaaggaatc cctagaatta gaggtaattc aagaggagac
    15481 caatatgtta aggttgtagt tgaaattcct aaaaaattaa atgataagca aaaagaacta
    15541 ttgagagaat ttgcgaagga gtgtgggtcg aacgtccacg aaaagaagaa aaccttcgga
    15601 caaaagatag aagatatgtt taagaaaaag tagttaaagt agccaatata taatcatatt
    15661 tttgaaatct tagttattta taagattgtc aaaggagata ttatgatttt tatattggct
    15721 atttttgtat aaaaaataaa tgtgcataaa ataaaactga atataaataa ttttctgaaa
    15781 aatacttgtc ctaaaatata taatgtgcta taatataatc aaataaaaga aaggggtgat
    15841 tccagtggga acggatgtgg cactgccaaa tgatgaagaa aactttattg taatatacaa
    15901 aataaaatta gatactttta gtgctagatt aagtactgga aaaactcatg agctgggata
    15961 attattgtaa ttacctagac tcaaactttt agtagtaaaa tagtaatatt atatagacaa
    16021 ggtatctaat ttttaaatct tttgtatgaa ttaaaatttt tataaagggt atatctgatt
    16081 agaatgtaat tagggaaacc tagttgtgaa ttacttttag tatatacatg tgtaaagaaa
    16141 tatgtatatg gtattggtta ttgtgctaga tgtgtatgta aaggaatatg tgtctagttg
    16201 ggtatatgta aagaaatata cttataatct atgaaaaatt tttagtataa attgctaaaa
    16261 cattgagatt gttttttata gaaagttagt ttataccaac taaaaatgtg cttaagattt
    16321 gtttgatgtt taattataaa taaggttgta gataaaaaaa taagtaaaga caaaggttta
    16381 aaatacttgc tttaaatata gtttgttttg aacacttgcc aaattaaaat aattaataaa
    16441 atagacatct tctcatagta aaatatactg tgagaggatg tttttgcttt gttcataaac
    16501 ttttatgaaa aatttgcata gtgtcacatt ttaatatact atatacataa taaaatatag
    16561 ataaaattaa atgattcaaa agagaaaggt gaaaagagat gtattatata ggtgtagaca
    16621 taggaggaac aggtatacaa gcaggagtag ttgataacta tgggaaaata atttttaggt
    16681 cagagtgtaa aacagtaatt gaaaaaggat ttgaaggaat acttaatgac ataaaaataa
    16741 tgatttataa gttgttggaa gataataaac ttactatgag tgacataaaa tctattggat
    16801 ttggtgttcc aggttttata aataaagaag gattagtaac ttgtgtaaat ttaaaatgga
    16861 ataaaaaagc ttttaataaa gagttaaaaa gaagatttcc agatgttgaa attcatggag
    16921 aaaatgatgc aacagtagca gcacttggag aagcaaagtt tggaagtatg aaaggtgcta
    16981 atgttggagt attatatact ttaggaactg gtgtaggtgg aggaatagta attaatcaaa
    17041 aagtcttttc tggagcacat ggattgggtt cagaaatagg tcatcaaatt ataggagaaa
    17101 actattttaa ttgtaattgt ggaaataatg gttgtgtaga aactttttgt tcagcaactg
    17161 caattataaa gtattctcaa aaactgatag aagaaggaga aaagagtagg attttagatt
    17221 tggcagaggg aaatttagaa aatgtaaatg caaagatggt gtttgatgca tatagagaaa
    17281 atgatttagt ggcaattaaa gtaataaata gatttaaaga gtatttagca aaaacttttg
    17341 caaacacaat aaattcttta gacccagaaa ttatatctat aggaggaggc atttctaaat
    17401 ctagcgatat aatacttgat ggaatagaag atttggtaag aaaatttgta ttatacaaaa
    17461 cagaagatat agctacaatt acatgtgcaa ctttaggttc agacgcagga attataggag
    17521 cagctttttt ataatgtaaa tgaactgctt attggaggcg tgatgatata tgggtaaaac
    17581 aaatgctaga agaacgcttt ttttgatagc aataggttca ggaactatgc tgaatcctct
    17641 taattcttct atgatttcac ttgctttaca tagtatacaa aatgattttc atattagttt
    17701 tacaactgta tcttggattg tttcagcatt ttacctagct agtgctattg cacaaccagt
    17761 ttcaggtaag ataggagatt taataggtag aaaggtctta tttttgagtg gtttgatgct
    17821 tgtaatttta tcagcatcca tagtaccact tgttcaatca ttctttatat tgatttttat
    17881 aagagtaatt caagcaattg gaacaagtac cctatatcca tctggagttg ctcttataca
    17941 aaataatata aaagaaagac aatcttctgc attagcagtt ctaaccattt ttgcttcaac
    18001 tacagcagga ttaggtccga cacttggagg attattgttg gacttaggag gatggcatgc
    18061 tatatttctt gtaaatatac ctgttgtttt ggtttctata tgcttagggt attttttatt
    18121 cccaaaagaa gtaaaagaaa agaaatctat aaaagaaact ttaaaaaatg ttttgtcaag
    18181 attagatatt attggcatac ttctattttc aattgcaatg atttttatat tactattttt
    18241 gctatctatg aaggagtctt ttaacttaga acaacttgta tttggtataa tactgatgtg
    18301 tctttttatt tggcatgaat tgagaacaaa attgccattt atagatatta agctttttgt
    18361 aagtaatcct aaattgtcaa aagtttactt gcaatttatt atattaaatt tctttaatta
    18421 tattttgttt tttggtcttc ctagttattt tcaagatgcc ttacattata gtgcaaagtc
    18481 gactggatta tttatgctat ttatgtctgg tataggtata tttatatctc cattaactgg
    18541 aaaatggata gataaatctg gaactagatt tcctcttgtt acatcatcaa tctttatgtt
    18601 tattagtgca gtacttcttt cagtagtttt tatccatata cctgtaatag gaaagggtat
    18661 tatattatca ttagcaggta ttagttatgg agttggtaat gtagccctgc aatcctctat
    18721 gtttgaagaa agtcctcgtg acagtatagg gacagcttct ggtctgtttc aaactagtcg
    18781 ttatttaggt tctattttag caactgtttt acttggaatg gtatttgaag taaatataac
    18841 atcagaacaa tttcagatat taggttatgt tatggtggtc ttaggtacaa taagtttttt
    18901 attaaatttt atattaaaga aagaattaag aaaagtagaa tagtggattt atggatttaa
    18961 taagtctaat aactgataag tttaaaaata ttgtttgtaa aacataactg gtttgatatt
    19021 tattaattct aagttttgaa gcatagagta ctcgataagt atacaatatt ttgaattaaa
    19081 attaagcaga aatttgaact agtctttcaa atctctgctt aatttagtgg ttaaattttt
    19141 ttatagagaa aataagattt gacacttaga taaaaaatca ttgcattttc aaaattcttt
    19201 aaatcaaacc caagtatctc atta
//
LOCUS       DAEMHW010000062        19018 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEMHW010000062 DAEMHW010000000
VERSION     DAEMHW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19018
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.062"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..2018)
                     /locus_tag="KPK67_001843"
     CDS             complement(<1..2018)
                     /locus_tag="KPK67_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2458812.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENN"
     gene            2408..3076
                     /locus_tag="KPK67_001844"
     CDS             2408..3076
                     /locus_tag="KPK67_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458813.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            3078..3563
                     /locus_tag="KPK67_001845"
     CDS             3078..3563
                     /locus_tag="KPK67_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG2458814.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            3624..4745
                     /locus_tag="KPK67_001846"
     CDS             3624..4745
                     /locus_tag="KPK67_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBG2458815.1"
                     /translation="METNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            5026..6216
                     /locus_tag="KPK67_001847"
     CDS             5026..6216
                     /locus_tag="KPK67_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2458816.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYKMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    6350..6605
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6664..7665
                     /locus_tag="KPK67_001848"
     CDS             6664..7665
                     /locus_tag="KPK67_001848"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBG2458817.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            7769..8158
                     /locus_tag="KPK67_001849"
     CDS             7769..8158
                     /locus_tag="KPK67_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBG2458818.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLFFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            complement(8325..9443)
                     /locus_tag="KPK67_001850"
     CDS             complement(8325..9443)
                     /locus_tag="KPK67_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2458819.1"
                     /translation="MNIKSAFIRKRGEKFHVYVEYVEEETGKKKQKSYGSYEKKKDAE
                     KHLIEIKSTINNNKFVAPNSVTLVERCYIYLEEKKDDFSPYTLRNRRSVIRNHIEPFF
                     GDMKLIDISPNILQTYVNKIYKKYSLNSAKNSISFLTALLHEAYRLREIQEDVSSFVI
                     TPNKKDTSVTNFYTKEEAQLLLERCLDTDLAIPIYFMLGLGLRFGEAVGVRWCDVQLN
                     EGIINVKQTMVHVDGKVTFKSPKTNKSKRRLTAPTELIILLKEEKLRQNKLKLQGILK
                     NELDLICLNKKFQPWTQQKFFKPFKRLLESNNLRYIKLHELRHTNATLMLLSGTNIKT
                     ISERLGHTDIKITMNKYSHVLEEMDKEASENLSKILFK"
     gene            complement(9511..9912)
                     /locus_tag="KPK67_001851"
     CDS             complement(9511..9912)
                     /locus_tag="KPK67_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBG2458820.1"
                     /translation="MKKDIYVYPAILSYDADGISVEFPDLPGCYTCADSTEEALKMAK
                     EALGLHLYGMEEDDDIIPNPTTIDKLNLEKNQIPTLIEIYMPIHRKAIENYSVKKTLT
                     IPQWLNREAEKHKVNFSQILQEALKNYLNIH"
     gene            complement(10367..12031)
                     /locus_tag="KPK67_001852"
     CDS             complement(10367..12031)
                     /locus_tag="KPK67_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBG2458821.1"
                     /translation="MNNENEQLKSDLLINDEKIKNLNFKIPWYYSLWTISILILSTFS
                     TYSISFIVAIIFLFKRNKIMKKHKDSISILLSDVEKINNKYILLNDEIKMKEKHFEDL
                     CESNENKLKELSNLLDKKKVEIDKFDSENQDKFKLIEELKIEKERLDNLIKDKNILKD
                     NINTLNSHLEELKNEREELRDINTTLKNKKEELKRLSEELIQTEDEVLLQSFGLYNPK
                     YDFENSDEYMKKLKEIREMQKLLIRNKTGVKYSDSWTVDGSVQKGRTMTNQNIKTALK
                     LFNSECDIAMSKVSFKNIDSIEKRIRKAFTDTNKLNTSNKVSIKENYLNLKIDELYLY
                     YEYLQMKEEEKEEQRALREQMKEEALVQKEIENQKRKLKKEELQFKNELLRLKSTIPE
                     DEKDKLEWEQKINSIEEKLALLSKDLDDVLNREQNTRAGHVYIISNIGSFGENIYKIG
                     VTRRLDPTERINELSSASVPFKYDIHVTIFSEDAPKLESALHKAFDNKRVNKVNNRKE
                     FFKVTLDEIRTEVEKNFDKTVEYTKLAEAQEYRQTLKIQELNNKLA"
     gene            complement(12301..13749)
                     /locus_tag="KPK67_001853"
     CDS             complement(12301..13749)
                     /locus_tag="KPK67_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HBG2458822.1"
                     /translation="MSILNLFRKNKNKNNVDCNEKSNESKQNYNDSTTLSLECNSNSP
                     HNIEVKNEINKVLNVSCNDTTNLYELEKQAREKSDALYDIHTNSINKFNPQNNILETD
                     TKPLTSIEKSFLKYIIGENIYEPYIATYWTYEYNINYSYLISKFFNMDYLKISNYIED
                     LTKLTVSELKEILKSNNIKSTGKKAELIERIEKEISCKDLSNFFNSSNKYYALTDKGK
                     ELLKDVRKSVTKNTDLEDQCLELIYIDKYEEAYDLICKYESSKNIQRGININWENHKI
                     TPMKIKSYKAIKELDINLKDTLLDNIIKSSYILCDMLGNNSKTSILVKRLVGEKIDNI
                     EINNAINSINDVIYNHSTIAYNANDSINNKNLHYMDIVKNNKSVPFEVNNKNYNFKIN
                     ENEIYFFNILKIKMYENELKNNFVFDRMSDGAFNVFDTSDNFIGKVKLQGRKKWIMYT
                     KNEFGSEHIYGELIHLIDGIDAWIKYSKEYLK"
     gene            complement(14393..14743)
                     /locus_tag="KPK67_001854"
     CDS             complement(14393..14743)
                     /locus_tag="KPK67_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2458823.1"
                     /translation="MTRSDIMFSNRLRELRKQKGLTQMELAKLLNCSLSKIAMLETDK
                     RDPVKEDLLRFSEIFEVSIDYLLGKNNLDLNPRLNKEMEQALHKLYSLDEENRKAIEK
                     IIDNAYYKIINEEK"
     gene            14887..15075
                     /locus_tag="KPK67_001855"
     CDS             14887..15075
                     /locus_tag="KPK67_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009587961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2458824.1"
                     /translation="MKELSKFRKNLGLTQKQLAERIGISKSYYSKIEGNFKKPGRGFL
                     EKFKNTFPNEDMNIFFKQ"
     gene            15109..15903
                     /locus_tag="KPK67_001856"
     CDS             15109..15903
                     /locus_tag="KPK67_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBG2458825.1"
                     /translation="MNNLQVIERNNERVLTTQQLADVYETDVNNIQANFNRNKDRFKE
                     NIHYFLLQGEYLKEFKNQPTNSQLVSKHSSQLYLWTERGANRHCKILDTDKAWEQFDN
                     LEETYFKVKQQKPTCIEDVLIESLKEMKDLRLQVNQANSIALEAKTEVKTIKEVVSLN
                     ATDWRKDTQQLIARIAKKQGGFEHINMLRRESYELLDNRFGVDLHRRLINKRRKLAEE
                     GVSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSNN"
     gene            16078..16728
                     /locus_tag="KPK67_001857"
     CDS             16078..16728
                     /locus_tag="KPK67_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458826.1"
                     /translation="MNNLQIFKNCNFGEIRTIEIDGKPYFVATDIAKALGYSNTRDAV
                     KKHCKWVAKCDIPHPQSNSKVLKVNVIPEGDMYRLISNSELPNAEKFESWVFDEVLPS
                     IRETGKYQLQQDVITELTGTIGDLKGTIKEYKKLCKITCSKKQQYSKYIKNRLDIDKA
                     NKEYNQVKARLFLILGVEKWEDIDFDTSNNLIQIIDDCIKVIKSERPYKQLSYFEN"
     gene            complement(16883..17284)
                     /locus_tag="KPK67_001858"
     CDS             complement(16883..17284)
                     /locus_tag="KPK67_001858"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458827.1"
                     /translation="MKNTDEILIAKVIDPYNLVINKGSKDGIKEKQKFLIYSIDKENP
                     IIDPKTHQELGYLEIIKGTGVVKHIQENFTTILSCEYNSIPTKSVTKPIHKSLAYKMG
                     FEPIYVTEETSTEEELLAFENPEVGDFAKPI"
     gene            complement(17288..17785)
                     /locus_tag="KPK67_001859"
     CDS             complement(17288..17785)
                     /locus_tag="KPK67_001859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458828.1"
                     /translation="MLNTSTFISTIIAFIVGILVNSLAYEIQVPFWLFSGYIIFSLIC
                     FWFLLIKINNKSSSTFDLSDIELIKFIHNSHKDQLSCILKSCPYINRETLLTLFYIED
                     DLERYFATGKVINIQSNGLIHITILDLPPEYLLNDIHNNNIKLINSIIIKPIVTQTFL
                     SERSI"
     gene            17973..18305
                     /locus_tag="KPK67_001860"
     CDS             17973..18305
                     /locus_tag="KPK67_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2458829.1"
                     /translation="MSTALQFIDTKDLVQELMQRDDTTDIIKMFLDRQGIKRMNLVTV
                     KEFSEYLKVSDSTARNMIREAMAQNHYTVIPLGSSYRIDLISFEEYVMKNALKDRNVM
                     KKTRKEVI"
     gene            18407..18607
                     /locus_tag="KPK67_001861"
     CDS             18407..18607
                     /locus_tag="KPK67_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458830.1"
                     /translation="MKIIYKNKVYKVEQDKKLFRITYYDEQKSNKKFNKDKKVKRSTL
                     TRDIELVNTYLPANLKIKGEEK"
     gene            18604..18849
                     /locus_tag="KPK67_001862"
     CDS             18604..18849
                     /locus_tag="KPK67_001862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458831.1"
                     /translation="MKSSKELINDVREYRKSSYLKYLSIVQRAWADRSLTADEQDRIK
                     QEAYAEYKRIERDTEEAEELLMREEFETDRPLAVQIM"
BASE COUNT         6993 a         2219 c         2876 g         6930 t
ORIGIN      
        1 atattatttt ctattatttt tatgcctttc tcagttattg atgagccttt tcttccttta
       61 ctagaaatta agtatccatt acgtgtcaaa tattctagca tatttcttac ttcgtattct
      121 gtgacattat attccataaa tttcattaac tttgtcctcc ccattggttt catgtgcaat
      181 gattttagga tagaaatgca tatgtttttg tcatattggt ttaaatcatt gaatatggac
      241 atattacttg acatatgatt attatttaat tttgaagata tgttttgagg taaatcattt
      301 attgtaagat aattggaacc catgtaagcc atatattcaa cacaatttct cagttctcta
      361 atattcccca tccaagtgta tgaatttaaa gcttctaaaa gactcttatc gagttttatg
      421 tttttatagg gaagttcttt taaaaatgat tctaaaagaa gaggtatatc ttctaatctc
      481 tttctaagag gaggtaaatc taaagtgaac atacttatcc tataaaataa atccatccta
      541 aatttatttt ctataattaa tttctctaaa tctttatttg tggcagctaa tattctcaca
      601 tcaattggaa taatacttgt accaccaatt tttctaattt ctttttcttg taagactctt
      661 aaaagtttaa cctgtaaatc atgtggcata tctcctatct catctaaaaa tatagtgcca
      721 gtgtgagcta attcgaataa tccttttttt ccaccttttt ttgctccagt aaaagaacca
      781 tcttcatatc caaatagttc actttcaagt aattcacttg gtaatgaagc acaatttatt
      841 gctacaaaag gtttattttt tctatttgaa tgattgtgta ttgactgagt aaaggcttct
      901 ttacctgttc ctgtttcacc tgttattaat ataggattat ctgtaagagc catttttttt
      961 gcctttttaa tacaatcttt tataatagaa ctatttccaa taatattatt aaatttatat
     1021 ttagctacat aacctctttt gacagaatca cttcttattt tttgttctaa tatttctata
     1081 ttgtttacat cttttaatgt aattaagcaa ccctctatat ttttataaag ataaaatggt
     1141 tttttagaaa gtatgaattt tttatctatt tcgtctatgt atataatcat attatctata
     1201 tttaagttct ttgtaatttt atctaagaaa ctctttggca tatattccat taaggaaggt
     1261 gattttataa gtttttcatc tagttccaac atattaagta aagacttatt tacaaaagta
     1321 accttgttaa aggtgttgaa aaatattaga ccatcaccta tttcatcaat ggtcatgtaa
     1381 agtatagttt ttaatttaga tatattatct aatgatgtat ttaagaaatc atgacttagt
     1441 gtctccttgg acaatttgta tagtttttct ataaatttct cattttttag ttttaatact
     1501 actaataatg acatatatgt atctaatgag atttttctcc aacctaaatc aacaatattt
     1561 tttatgtatt ttggtataaa tctaatctga cctggtgtta tagctgtatc taaatcatat
     1621 atttcatcac aatctggata tgtcggtaca aaatctatat gatttatacc aattttatat
     1681 agataagtag ccatctcaaa tgctgaagtt ttattggttg tcataagtaa cgattttgta
     1741 cctggaggta gtttcatcat tttttcatat ccaagttttg aaagtgcttt cgttccatga
     1801 ataatttcaa tatcttcctt aaaatgtttt attaaaaaat tactagttaa aggtgataga
     1861 gaaagcacta catctccctc taaattttct tttattccat caagtatatt ttgtgtttct
     1921 atgattattt cgtctccaaa aaagtttgtt aattcttctt tgtagtggtc actattcaat
     1981 ttatcatatg ttataaggat taacttcttt ttatccattt tatttctcct aacaaaatag
     2041 tatttatttt taaagtattt aaagtataag gcaagttata ttatacaaca aatagtagta
     2101 taaatttata tatttgatta aataataggt ttttttagga ttttatccta taaaaaccta
     2161 ttttaaaatt atttttgcaa gataaggaaa atagcttttt atattgtaat gtatatttta
     2221 aataattaat aaaaaaatat tatattttta aacttgaaaa gtaagtaatt ataagctatt
     2281 taatcgctgg aataaattta caataaaaaa ataaatacaa aggctttatc attaaggtat
     2341 tggcataagt tttgcttatt aaacaagtat actaataaaa ttcatttaaa caggagggag
     2401 aatttatatg agtaaattag aatcaacaac agaaaaagtt gaggtaaaat ggtatgggta
     2461 tgtagcatta atattgggcg cattattttt ttcaggaata tttaaagatg caccaggacc
     2521 attaaaagta ttggatttta acaatgtatt aggttctttt ggaacattgg gaacaattaa
     2581 tgatggtgta ggtacattag ctgcaaactt tagaggtgat ggaggtactg gccctcgaga
     2641 tggatggcta tatgcattga cattaatacc atcagttatg tttgcacttg gagtagttag
     2701 agttatagac cacttagatg gaatgaaggc agctcaaaag cttcttagtc cactattaaa
     2761 gccattatta gggttacctg gatttgcagg actaactttg atagctagtt tacagtctac
     2821 agatgcagct gcatctatga ctaaagaact taaagatgat gggtatatag atgaaaaaca
     2881 aaaagctgta ttttgtgcat ttcaattttc aggtgcgtct gcaattacga atttctttgc
     2941 ttctggagca gcactatttc catttatagg agatgtacca atatttatac cacttgcttt
     3001 aatattaatt atgaagtttg ttggggcgaa cttacttaga ttatatttaa ataaatttga
     3061 caagggggaa gcttaacatg agtgaggtag caaaaaaaga agtaaaagtt aaaaaaggaa
     3121 atgtgataga tgcgtttatt ggaggagcta gaaatggatt ccaaataagt acaaattcaa
     3181 tggctccaaa tgtcatcttc ggttttgcta taatttctgt gttcaattta acaggtcttt
     3241 tggatgtaat agggacaata tttacacctg taatgagtat ctttggactt ccaggtgttg
     3301 cagcaacagc tataatgaca atatttatat caatgggagg agcatctgga gtaatcgcag
     3361 gtttatttac agcaggtcaa ataagtcaag cacatgttgc aattctattg ccaatgttat
     3421 tgctttcagg agcgcagata caatttatgg ggagactttt aggaacagct gagttaaaaa
     3481 ctaaatatta tccacatatg tttgttatag caactttaaa tggttgtatt gcaatgctta
     3541 taatgaatta tttcgtagta taggtaatta taataaagat ttaatataaa tggaaagttt
     3601 aaattttaag gaggagctag attatggaaa ctaacaaaga ttatattgag tatgtacata
     3661 aagtgtatga ttatctacac actataccag aaaaaggatt tgaagaatat aagacatcag
     3721 agtttgtttt aaaagagctt gaaaaaatgg ggtttgaaaa tattaataaa ggagttgcag
     3781 gaactggtat tatagtaact atagattcta aaaaagaagg accagtattt gctataagag
     3841 cagatatgga tgcattggaa tttatcatag atggagaaaa gaaaatgata catgcttgtg
     3901 gtcatgatgg tcatacttct atgcttttaa cagctgcaaa gttaataaaa gagagaaatt
     3961 tagtaaaaag aggtaagtta aaaatacttt ttcaaccagg tgaagaaaaa ttatatggag
     4021 cattaagagt aattgattct ggtttgttgg atgatgtaga agaaatggta gggatgcatt
     4081 taagacctgg acaagattgt aggttgggaa aggcgatacc tgcaatgtac cattgtgcta
     4141 gctgtataat ggaatttacg attaaaggta tcacttgtca tggttctaaa ccacatttag
     4201 gagtaaatgc agtagatgct gtctgtttag cagtgaatgc aataaatgca ataagagtaa
     4261 atccagctat accaaattct ataaaagtaa caaatatatc agttaaggga gaaacttata
     4321 ataatatacc agatgaagca tttatggctt tagatataag ggcagaaagt aatgatataa
     4381 tagaagaatt aaaagaaaaa gcaatacatg ctgtaaaaac tgctgtaagt tctataaatg
     4441 cagaagctgt actaatttcc aaagatggag taccagcagc agaatttgat gaagaacttg
     4501 taaacttggc tgaagaaact ataaaagatg tattgggagc tgatgcatct ataggtatat
     4561 ttaaaaattc tggtggagaa gacttccatt attatacaca aaaactaaaa tgtaaaacta
     4621 catatattgg attaggagct gatgctacac caggatttca taatccaaat gtaacatttg
     4681 atacgaaagc tttagaatat ggtgtagata tatggtgtag attggttgaa aagagattgg
     4741 gataatatcc aaactaataa ttctacattt aaaattataa ttatagaagt tatagaaaaa
     4801 gattgcttat gttaattaga aaataattaa tatgagcaat cttattttga tataatgaaa
     4861 ctgattttgg aatattgaaa aaatatgatg gaaaagtaat tatattttaa atctattgga
     4921 caaaaaagtg atattattat aatattaaga attgtaaaat aataccaaag tgcatctagt
     4981 aatagactta acaattataa aaattttatt cttaggaggg aaaaaatgag taagtataat
     5041 tttgacagag taatagatag agtaggaaca gattgtgtaa aatgggattt tagaactaat
     5101 tgttcaacaa aagcacaaaa agatggtttg cctttttgga ttgctgatat ggattttgaa
     5161 tgtgcagaac caataataga agcattacgt aaaaaagttg aacataaaat atttggatat
     5221 agttcaaatg atagtgataa atactttaat gcagtttgtg attggtataa aaggagattt
     5281 aactgggaaa taaatagaaa agatattatt tttagtcctg gtgttgtacc tgctgttgct
     5341 attttagtaa gaatattaac taattctaat gaaggtgtaa tagtccaaaa accagtgtat
     5401 tacccatttg aagctaagat aaagagcaat aatagggacg ttgtaaacaa tcctctaata
     5461 tatgagaatg gtacttataa gatggattat gatgatttag aagaaaaagc taagtgtagc
     5521 aacaataaag tactgatact ttgtagtcct cacaaccctg ttggaagagt ttggagagaa
     5581 gatgaattaa aaaaggttgt agaaatatgt aaaaaatatg acctatggat aatagctgat
     5641 gagatacatt ctgatttaat tagaaaagga tttaaacata ctcctttaca gtcattatgc
     5701 ccagagtaca aggataaaat agttacatgt atagctccaa gtaaaacatt taatctggca
     5761 ggaatgcagt tgtctaatat aatagtgaat aatgatgaac taaaaaagaa atatcaagaa
     5821 gaggttacag ctgttggagt tgcaacttca ccaaatccct ttgctattgt agctacaata
     5881 gcagcatata atgaaagtga agactggctt aatgaattaa atgattactt agataataac
     5941 atacaattta ttgatgaata cttaaaagaa aacctaccaa aagtaaaact tgtttatcca
     6001 gaaggaacat atctagcatg gttagatttt actgcttatg gattaaatga agtagaactt
     6061 gaggatttaa tgtttaaaaa agctaatgta ttatttgatg aagggtatat atttggaaaa
     6121 gaaggaatag gatttgaaag aataaatgta gcttgtccac agtctttatt aaaagaatgt
     6181 atggacagat taaagactat atttgaaaat ttataaagta taggtaaaaa tttataaagt
     6241 gtttaaaaat aaaaaaataa taaacttgac aaattttaac taatcactta tcatactatt
     6301 atgaataaat agaaaacata agagagctaa attttattga attaaactct taaagacttt
     6361 gaaaggaaga gtagttatag aatgtaatta aagcgagttg gtgatggtgt gagaccaata
     6421 tgaagtctat aatgaagaac atcctggagt tgctaaccga aattttaaaa gagtaggctt
     6481 agtcggaacc aaaccgttat catattgggg tgtatcgagt aatatctgta cattgtgagc
     6541 tgggtttgaa tttatttcaa actaatcagg gtggtaccgc ggaaaagcaa cctttcgtcc
     6601 cttttatata gggatgaagg gtttttttaa tggaaaaata tataaatata gggagtgaat
     6661 gatatgagca caataatacc aaaggattat aaaagtagtt tgaacgtaat tgatacacaa
     6721 atagctataa aaaaattaaa agattttttt gaaatgagac taagtaatga attaaatctt
     6781 ataagagttt cttctccgtt atttgtattg ccagaaacag gtgcaaatga taatttaaat
     6841 ggagaaaagg ctgtaagctt tgatatacca tttatgaata aaaatgcaga aattgttcaa
     6901 tcacttgcaa aatggaaaag attagccctt aaaaaatatg ggtttgaagt tggtagtggt
     6961 ttatatacag atatgaatgc aataagaaaa aatgaagaat tagacaatat acattctctt
     7021 tatgtagacc aatgggattg ggaattggtt atagataaaa gtagtagaaa tgaaaagact
     7081 ctaaaagatg ttgtaaaaag attgtatgga gtatttaaag atactgaaat ttttgtatgt
     7141 agtatgtatg aaggtataaa agaaatatta ccagaagaaa taacttttat aacatcacaa
     7201 gagttagaag atatgtatcc agaattatca ccaaaggaaa gagaagataa aattgtaaaa
     7261 gagaagaaag cagtatttat aactcaaata ggaaaaacat taatatcagg agaaaaacat
     7321 gatggaagaa gtcctgatta tgatgattgg gaattgaatg gggatatatt attttacaat
     7381 cctgttttag attcagcatt agaattatct tcaatgggaa ttagagttga tgaagaatct
     7441 cttgatagac aattgaaact tgctggatgt gaagagagaa aagagtttga ctatcataag
     7501 atgcttttaa atggcgaact tccgtatacg attggtggag gaattggtca gtcaagaata
     7561 tgtatgtatt tcttacaaaa agctcatata ggagaagtac aagttggagt atggccacag
     7621 gatatgattc aaaattgtag tagtgcagga attgaattat tataatttaa atataaacta
     7681 attgataaaa tttatatcta atatgttcat caaattaatt atatcggtta taatgtagaa
     7741 taacctataa ttataagagg tgataaatat ggagttttta aaaataatca ttaatatagt
     7801 tttagatatt ttaaaaaaaa ttttggtcag atttaaaaat gctaaatttg gtttgttttt
     7861 tgtatttgat ttacttaaac tacctgattt tatgacagat aaaagaatca atatagttga
     7921 taaaataaag gtaatttctg tattaatttt tactatatcc tactttgtat ctggggttga
     7981 tataattcca gagatgattg caggggcatt tggttttata gatgatgcta tagttttaat
     8041 ttggagtata ggtatagtga atgaagaaat taataagtat agagttatcg caaaaaaaga
     8101 taaacattca aatataattg aaaatgtaga attttctata aaagatgaag aagaataaca
     8161 atttatatgt gttatgctta gatattatat aatactaagt actactagat agtagttaaa
     8221 tactagcttt tgctactatc tagtaataaa tatttgactg acatttaact gacaaataaa
     8281 aaaatatgtg attaactgac atcttactga cataaaactg accattattt aaatagtatt
     8341 ttgctcaaat tttcagatgc ttctttgtcc atttcttcaa gtacatggga gtatttattc
     8401 atagttattt ttatatctgt atgccccaat ctttctgaaa tagtttttat attagttcca
     8461 gaaagcaaca ttaaagttgc atttgtatgt ctaagctcgt gcaattttat gtatctaaga
     8521 ttgttactct ctaataatct tttaaaaggt ttgaaaaatt tttgttgtgt ccaaggctga
     8581 aattttttat ttaagcatat taaatctaat tcgtttttta gtatgccttg taattttaat
     8641 ttgttttgtc ttaacttttc ttcttttaat agtattatta gctcagttgg tgctgttaat
     8701 cttctcttag atttgtttgt tttaggagat ttaaaagtta cttttccatc aacatgtacc
     8761 attgtttgtt tcacatttat aattccttca ttaagttgaa catcacacca tctaactcct
     8821 acggcttcac caaagcgtag accaagtcct aacataaaat agataggtat agctaaatca
     8881 gtgtcaagac atctttctaa tagaagctga gcttcttctt tagtataaaa attagttaca
     8941 gatgtatctt ttttatttgg agttattaca aaactagaaa catcttcttg tatttctcta
     9001 agtctataag cttcatgtaa taaagcagtt aaaaaactaa tagaattttt tgcagaattt
     9061 aaggaatatt ttttatatat cttattaaca tacgtttgta aaatatttgg actaatatca
     9121 attaatttca tatctccaaa aaaaggttca atgtgatttc taattacact tcttctattt
     9181 ctcaaagtat aaggtgaaaa gtcgtctttt ttttcttcaa ggtatatata acatctttct
     9241 acaagagtca cactatttgg agcaacaaac ttattattgt ttatagtaga ctttatttca
     9301 attaaatgtt tttcagcatc ctttttcttt tcatagcttc cataactttt ttgtttcttt
     9361 ttgccagttt cttcttccac atattccaca tatacatgaa atttttcccc tctttttctt
     9421 ataaaagctg atttgatatt catatatgta cgccccttta taagtttata tattttatat
     9481 aaaagagcag ttaaactgct ctaattatat ctaatggata ttaaggtaat tttttaaagc
     9541 ttcttgaagt atctgggaaa aattaacctt atgtttctca gcttctctat taagccactg
     9601 gggaatagta agagtctttt taacagaata attctctata gcttttctgt gaataggcat
     9661 atatatctct attaaagttg gtatttgatt tttctctaag tttaatttat ctatagtcgt
     9721 tggattaggt attatgtcat cgtcttcttc catgccatac agatgtagtc ctaatgcttc
     9781 ttttgccatt tttaaagctt cctctgttga gtctgcacat gtgtaacagc ctggtaaatc
     9841 gggaaattct acagagatac catccgcatc ataacttagt atagcaggat atacatatat
     9901 atcttttttc attttatcaa ccccttacag ttacattata aaacgtactt acatacgtgt
     9961 aaataagaag aacaaactat aatagaaatt taaatgtaat atataggtaa aatatgtaag
    10021 aattatatga tataataatt gtagcaagga aaataaaatc gaaaagctaa gggtgatttt
    10081 ctcatataaa cgccaaattc caatatagga aggaggtgaa tttatatgtt aattaacttt
    10141 ttattgagta tagtagctgg tattgtatca gcttacattt acgagaaaat aaaaaatcac
    10201 ccaaacgcca ataagggtga tttaaagaaa taacttttaa attctaatca attagagaaa
    10261 ttcacctttt acaaagtaaa ttttatctcc ttgctattat tatatcacaa attggtatag
    10321 atattcaaaa ataatatttt attaaagtat aattgtttga atatatttaa gctaatttat
    10381 tatttaattc ttgtattttt aatgtttgtc tatattcttg tgcttctgcc aattttgtat
    10441 attccacagt cttatcgaaa tttttctcaa cttctgttct tatttcatca agcgtaactt
    10501 tgaaaaattc ttttctatta tttaccttgt taactctctt attatcaaaa gctttatgta
    10561 aagctgattc taatttaggt gcatcttcac taaatatagt cacatgtata tcatatttga
    10621 aaggaactga tgcactactt aattcattta ttctctcagt tgggtctaat cttctagtta
    10681 ctcctatttt atatatattt tctccaaaac taccaatatt agagattatg tatacatgtc
    10741 ctgctcttgt attttgttct ctatttaata catcatctaa atcttttgat agtaaagcta
    10801 gtttttcttc tatagaattg attttttgtt cccattctaa tttgtctttt tcatcttctg
    10861 gtatagttga ttttagtcta agtaattcat tcttgaattg taattcttct ttttttagtt
    10921 ttcttttctg attttctatt tctttttgaa caagagcttc ttctttcatt tgttctctca
    10981 aagctctttg ttcttctttt tcctcttctt tcatttgaag gtattcataa tataaataaa
    11041 gctcatctat tttcaaatta agatagtttt cttttataga aaccttattt gaagtattta
    11101 atttatttgt atcagtaaat gcttttcgta ttcttttttc tatagaatca atatttttaa
    11161 aactaacttt tgacatagca atgtcgcact cactattgaa gagtttaaga gctgttttaa
    11221 tattttgatt agtcatagtt ctaccttttt gaacacttcc atcaacagtc caagaatcag
    11281 aatattttac acctgtttta tttcttatta atagtttttg catttctctt atttctttta
    11341 attttttcat atattcatca gagttttcaa aatcatattt tggattatat aatccaaatg
    11401 attgaagcag aacctcatct tcagtctgta ttaattcttc tgataatctt tttagctctt
    11461 cttttttatt ttttaaagtt gtgtttatat ctctaagctc ttccctttca ttttttaatt
    11521 cttctaaatg agaatttaaa gtattaatat tatctttaag tatattttta tcttttataa
    11581 gattatcaag tctttctttt tctattttta gttcttcgat aagtttaaat ttatcttggt
    11641 tttctgaatc aaatttatca atttcaactt tctttttgtc taatagattg gataattctt
    11701 ttaacttatt ttcatttgat tcacataaat cttcaaagtg tttttctttc attttaattt
    11761 catcatttaa taaaatatat ttattattga ttttttcgac atcagataat agtatactaa
    11821 tgctatcttt atgttttttc attatcttat ttcttttaaa taagaatatt attgcaacta
    11881 taaatgatat agagtatgtt gaaaacgtag aaagaattag tatggatata gtccataaag
    11941 aataatacca gggtatcttg aaattaagat ttttgatttt ttcatcgttg atgagtaaat
    12001 cactttttaa ctgttcattt tcattgttca tatcaaaact ccttttttat ttttgttcta
    12061 ctttatgata ataataattt aagtatataa aattttatgt gcaatttcca tatatttgca
    12121 aaaaaaggta aaaataaaac aaaatggtag ttatttaaaa gaatttagtt ggattataaa
    12181 ggtatgttaa cagaaattag aaaaataata taacattctt ataaaagtat gttatcagta
    12241 ttttgaacat aaggaaaagc cacttaaaat tagttttaat cattttaagt agctctttta
    12301 ttattttaga tactcctttg aatatttaat ccaagcatca ataccatcaa ttaagtgaat
    12361 taattcgcca taaatatgtt ctgaaccaaa ttcatttttc gtgtacatta tccatttttt
    12421 tctaccttgc aatttaactt taccaatgaa gttgtctgaa gtatcaaata cattaaatgc
    12481 tccatctgac attctatcaa agacaaaatt attttttaat tcattttcgt acattttaat
    12541 tttaagtata ttaaaaaagt agatttcatt ttcattaatt ttgaagttgt aatttttatt
    12601 attaacttca aatggtactg atttgttatt tttaacaata tccatataat gtaaattttt
    12661 attattaata ctatcatttg cgttatatgc tatagtagag tgattgtata ttacatcatt
    12721 tatactattt atagcattat taatttcgat attatcaatt ttttctccta ccaaacgttt
    12781 tactaatata gaagttttgg agttatttcc taacatatca caaagtatat aggatgattt
    12841 aattatgtta tctaaaagtg tgtcttttaa atttatatct aattccttta tggctttgta
    12901 agacttaatt ttcataggtg taattttatg attttcccag tttatattaa ttcctctttg
    12961 gatattttta gaggattcat atttacaaat taagtcatat gcttcctcat atttatcaat
    13021 ataaattaat tctaaacact ggtcttctag gtcagtattt ttagtaacag atttacgcac
    13081 atctttcaac agttcttttc ctttatctgt taaagcataa tatttgttgg agctattaaa
    13141 aaaattagat aaatctttgc atgagatttc tttttctatc ctttctatta attcagcctt
    13201 tttaccagtt gatttaatat tattagattt taatatttct ttcaattccg aaactgttaa
    13261 cttagtaagg tcttcaatgt aatttgaaat cttaagataa tccatgttga aaaacttaga
    13321 aattaaatat gaatagttaa tattatattc atatgtccaa taggttgcta tataaggctc
    13381 gtatatattt tctccaatta tgtattttag gaaagatttt tctattgatg taagtggttt
    13441 agtatctgtt tctaatatat tgttttgtgg gttaaactta ttaatactat tagtatgtat
    13501 atcatataaa gcatctgatt tttctcttgc ttgtttttct aattcatata aatttgttgt
    13561 atcattacaa cttacattca gtactttatt aatttcattt tttacttcta tgttatgagg
    13621 tgaattagaa ttacattcta gtgaaagtgt agtactatca ttatagtttt gttttgattc
    13681 gttcgatttt tcattgcaat caacattatt tttgttttta ttttttctaa atagattcaa
    13741 aatgctcata aaaaattact ccttttgtat ttaattaatt tctagtgtat tctactttgt
    13801 gacaataaca atttaagtat ataaaacttt ccgtataaat gctatgtata ttaggaaaaa
    13861 acatagaatt aaatagtttt gaaaagaata ctatatttat atggtataaa aagtaaaacg
    13921 aagattttaa aattaaataa taaaaaatat tataaaaaca agaaaataca aaaaattata
    13981 atcaaaaaga cgattaataa tatacaggaa tggaaaaaat aaccaactgt ctacaaattt
    14041 cgattttttt gttacaattc cccttttttt attgcatata tttaacaaaa gtattattat
    14101 gtaagtaaga taattatcaa agacaattct gaataatcta aaaatatttt gggggttgaa
    14161 agggtgtaat tatataaact ttattaaaaa ttgttgggag tttttaacta aaaataagaa
    14221 cataagttct aaaaataggg ggatggtata gttgaagaaa gacgagtatt taaaaaagaa
    14281 taaatctaaa ttagaaaaga ttcttaaagc agaacctaaa aagtatgaat acataataaa
    14341 attaatagaa gaagaaatga gtaaaaaaaa taaaagagtg taaaaagctc ttttattttt
    14401 cttcatttat tattttatag taagcgttat caataatttt ctctattgct ttcctgttct
    14461 cctcatctaa ggaatataat ttgtgtaaag cttgttccat ttctttgttt aatcgaggat
    14521 ttaagtctag attattcttc cctaaaagat aatcaattga tacctcaaaa atttctgaga
    14581 atcttaataa atcttcttta acagggtctc ttttatctgt ttctagcatt gcaattttac
    14641 tcaaagaaca atttaataat tttgccaact ccatttgtgt taaacctttt tgttttcgta
    14701 actctcttaa tctattagaa aacataatat cactccttgt catatttata ctacgattgt
    14761 atcactgtta gtgatacaaa taaatctttt tttaaaaaac ttcacgaaaa gagataaaaa
    14821 gtcttgacta tattcactta aagtgatatt attaatttat cacaaatagt gaaggcgagg
    14881 tgtaacatga aagagttatc aaagtttaga aaaaatttag gattaaccca gaagcaatta
    14941 gctgaaagaa taggcatatc taaatcttat tattcaaaga tagaaggaaa ttttaaaaaa
    15001 ccaggaagag gatttttgga gaaatttaaa aatacattcc caaatgaaga tatgaacatt
    15061 ttttttaaac aataatatca ctttaagtga tgtgaggagg attataaaat gaataactta
    15121 caagtaatag aaagaaataa cgaaagagtt ttaactacac agcaactagc agatgtatat
    15181 gaaactgatg taaataatat acaagctaat tttaacagaa acaaagatag atttaaggaa
    15241 aatatacact attttttatt gcaaggcgag tatctcaaag agtttaaaaa ccaacctact
    15301 aatagtcagt tggtttcaaa acattctagt caattatatc tttggactga aagaggagca
    15361 aacagacact gcaaaatatt ggatactgac aaagcttggg aacaatttga caacttagag
    15421 gaaacatact tcaaggttaa gcaacagaaa ccaacttgta tagaagatgt attaatagaa
    15481 agcttaaaag aaatgaaaga tttaagactt caagttaatc aagcaaatag cattgcttta
    15541 gaagcaaaga cagaggttaa aacaataaaa gaagttgttt cattaaatgc taccgactgg
    15601 agaaaagata cacaacaact aattgcaagg atagcaaaaa aacaaggtgg atttgaacat
    15661 ataaacatgc ttagaagaga aagttatgag ttactagata atagatttgg agtcgatttg
    15721 catagaaggt taataaataa aagaagaaag ctggcagaag aaggtgtatc tgaatctaaa
    15781 agagagaaag ttaacaattt agatgtaata caagatgata agaaacttat agagggttat
    15841 gtagctattg taaaagatat ggctttaaaa tatggaatat caagtgattt aagtaataat
    15901 taggttaaat cataacagca cattgaaaac taaatataaa attttaaaag gagcaagtat
    15961 atgagaaata aagaaaaaga attaattatt agaggtattt taattggaat tttctatttt
    16021 ttaggaattg tatttagtga gattttttta aaataataaa attggaggat ggaaagtatg
    16081 aataatctac aaatatttaa aaattgcaat tttggtgaga taagaactat tgaaattgat
    16141 ggcaagccgt attttgtagc aacagatata gcaaaagctc ttggctactc taatactaga
    16201 gatgcagtta agaaacattg taagtgggta gcgaaatgcg acatacctca tccacaaagc
    16261 aattctaaag ttttaaaagt aaatgttata ccagaaggcg atatgtatag acttatctct
    16321 aacagcgaat taccaaatgc agaaaaattt gagagttggg tatttgatga agtacttcca
    16381 agcataagag aaacaggtaa atatcagtta caacaagatg taataacaga acttacagga
    16441 actataggag atttaaaagg cacaataaaa gaatataaaa agttgtgtaa gataacttgc
    16501 tctaagaaac aacagtattc taaatacata aagaatagac tggatataga caaagctaat
    16561 aaagaatata accaagtgaa agcgagatta ttcctaattc ttggagttga gaagtgggaa
    16621 gatatagatt ttgatacatc taataattta atacagatta tagatgattg tataaaagtt
    16681 ataaagtcag aaagaccata taaacaatta agttactttg aaaactaaat acagaatatt
    16741 caaaaagagg tgattatatg agaaataaag aaaaagaatt gttatttaga gggattttaa
    16801 ttataatatt ctttttttta ggagtaatgt taagtaattt tttaaataaa gtttaaaagc
    16861 ccttgtataa agagctttta atttaaatag gtttagcgaa atcaccaact tctggatttt
    16921 caaatgcaag taactcttct tcagttgaag tttcttctgt tacatatatt ggttcaaaac
    16981 ccattttata agctagagat ttgtgtatcg gttttgtgac acttttagta gggattgaat
    17041 tatattcaca tgaaagtatt gttgtgaaat tctcctgtat atgtttaact acaccagtcc
    17101 cttttatgat ttctaagtat cctaattctt gatgggtttt agggtctatt attggatttt
    17161 ctttgtctat agaatatatt aagaattttt gtttttcttt tataccatct ttagaacctt
    17221 tatttattac taagttataa gggtcaataa cttttgcgat taatatttca tctgtgtttt
    17281 tcataattta aatactcctt tcagataaaa aagtttgtgt aacaataggt tttataataa
    17341 tagaatttat aagctttata ttattattat gaatgtcatt tagcaagtat tctgggggta
    17401 aatctaaaat agttatatgt attaatccat tactttgaat gtttatgact tttcctgtag
    17461 caaaatatct ttctaaatca tcttcaatat aaaacaaagt taaaagtgtt tctctattta
    17521 tatatgggca tgattttaaa atacaactta actggtcctt gtggctatta tgtataaatt
    17581 tgataagttc tatatctgat aaatcaaatg tagaacttga cttattattt atttttatta
    17641 agagaaacca aaagcaaatt aaggaaaata ttatataacc tgaaaataac caaaaaggaa
    17701 cttgtatttc ataggctaat gaatttacta atataccaac aataaatgcg atgatggtgg
    17761 aaataaaagt acttgtattt aacatattat cagataaaaa tcgacttatc ctattagtat
    17821 ttttaatgaa aatcaacacc tttcaaataa aatagctaaa ttatttatag atatatctat
    17881 aaatataatt atactacatt tcatgtgtgt aaatttaaat tttagaaaaa aatggtaaaa
    17941 agtgattaaa gtatagaaag ggtgagataa aaatgagcac agcattacaa ttcatagata
    18001 caaaagactt agtgcaggaa ttaatgcaaa gagatgatac aacagacatc attaagatgt
    18061 ttttagatag acaaggaatt aagagaatga atttagtcac agtaaaggag tttagtgagt
    18121 acttaaaagt gtctgattca actgcaagaa acatgataag agaagcaatg gcacaaaacc
    18181 attatactgt aattcctttg gggagttcgt ataggataga tttaatctct tttgaagaat
    18241 atgtaatgaa aaatgcacta aaagatagga atgtaatgaa aaagactaga aaggaggtga
    18301 tttagttgag tgtaagagta ctaatagctt atgtacagtt ttgtaaagat aagcagataa
    18361 aagcaagttt tgaaggtctt aaaaaataca acaagggaga actagtatga aaataatcta
    18421 taaaaacaaa gtttacaaag tagaacaaga caaaaagtta tttagaatta catactatga
    18481 cgagcagaaa agtaataaga agtttaataa ggataagaaa gtaaaaagaa gtacattaac
    18541 aagagatata gagttagtta acacatattt accagcaaat ttaaaaataa agggggaaga
    18601 aaaatgaaaa gcagtaaaga gttaattaat gatgtaagag agtatagaaa atctagttat
    18661 ttgaagtatt tatctatagt acaaagagca tgggcagata gaagtcttac agcagatgaa
    18721 caagacagaa ttaaacaaga agcatatgca gagtacaaga ggatagaaag agatacagaa
    18781 gaagcagaag aactgctaat gagagaagaa tttgaaacag atagaccctt agcagttcaa
    18841 ataatgtaga aaagagccat cacgactggc tcaattcaaa taactattaa aaaatttaat
    18901 taagctaatt atagcacaaa tggagggaaa ttatgagtac tttatatgaa ttaactacag
    18961 atttattaga aatagaagaa ggtttaacag aaataacagg aaatgaagct gaaaaact
//
LOCUS       DAEMHW010000063        18893 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEMHW010000063 DAEMHW010000000
VERSION     DAEMHW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.063"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            346..1410
                     /locus_tag="KPK67_001863"
     CDS             346..1410
                     /locus_tag="KPK67_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBG2458832.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            1568..2254
                     /locus_tag="KPK67_001864"
     CDS             1568..2254
                     /locus_tag="KPK67_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2458833.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKVKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            2244..3275
                     /locus_tag="KPK67_001865"
     CDS             2244..3275
                     /locus_tag="KPK67_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2458834.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            3357..4022
                     /locus_tag="KPK67_001866"
     CDS             3357..4022
                     /locus_tag="KPK67_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2458835.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLNGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            4035..6452
                     /locus_tag="KPK67_001867"
     CDS             4035..6452
                     /locus_tag="KPK67_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2458836.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKNKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     SYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASNKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(6633..7424)
                     /locus_tag="KPK67_001868"
     CDS             complement(6633..7424)
                     /locus_tag="KPK67_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2458837.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNIDVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(7457..8044)
                     /locus_tag="KPK67_001869"
     CDS             complement(7457..8044)
                     /locus_tag="KPK67_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2458838.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(8221..9612)
                     /locus_tag="KPK67_001870"
     CDS             complement(8221..9612)
                     /locus_tag="KPK67_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBG2458839.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDILKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(9802..11100)
                     /locus_tag="KPK67_001871"
     CDS             complement(9802..11100)
                     /locus_tag="KPK67_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBG2458840.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLKKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKDIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(11457..12332)
                     /locus_tag="KPK67_001872"
     CDS             complement(11457..12332)
                     /locus_tag="KPK67_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBG2458841.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(12525..13211)
                     /locus_tag="KPK67_001873"
     CDS             complement(12525..13211)
                     /locus_tag="KPK67_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBG2458842.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(13260..13793)
                     /locus_tag="KPK67_001874"
     CDS             complement(13260..13793)
                     /locus_tag="KPK67_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG2458843.1"
                     /translation="MSDLNNKKEQYENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(13837..14469)
                     /locus_tag="KPK67_001875"
     CDS             complement(13837..14469)
                     /locus_tag="KPK67_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBG2458844.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(14559..15638)
                     /locus_tag="KPK67_001876"
     CDS             complement(14559..15638)
                     /locus_tag="KPK67_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG2458845.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(15771..17117)
                     /gene="pepV"
                     /locus_tag="KPK67_001877"
     CDS             complement(15771..17117)
                     /gene="pepV"
                     /locus_tag="KPK67_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBG2458846.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(17186..18466)
                     /locus_tag="KPK67_001878"
     CDS             complement(17186..18466)
                     /locus_tag="KPK67_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBG2458847.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDIGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(18593..18853)
                     /gene="hfq"
                     /locus_tag="KPK67_001879"
     CDS             complement(18593..18853)
                     /gene="hfq"
                     /locus_tag="KPK67_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBG2458848.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
BASE COUNT         6634 a         2950 c         2223 g         7086 t
ORIGIN      
        1 aaatacatta aatccatcat tctcaaatgc ataaaaaaga ccttcattta agtttatatc
       61 atcttcaata attaataagt tcaattttac tctcctttag attttttaga taaataatta
      121 ttttcttgca ttatcatact aaaaatattt taattttatt tatttcacta aaaagattgt
      181 atttaaattt gttttcaaaa taaaaacatc ataatcaagc atatagttat atatttccca
      241 atttaattac gcttaaattg aaaatataac tttattctta tataattaaa ttatacaaca
      301 ataaagttat attttttaaa tatattttta ctagggggaa tagaattgaa aagggttgat
      361 gagagaaata ctatgtttgc aagagcaaat tacaaaaaag atagtactgc ttataatgat
      421 tattataaga aaaatcctga caaaaaagaa attgatgata gtataagaaa tagaccaaat
      481 ttatgtagtg aaggaacaat gacttacaat gagttaaact ctccaatggc aagtagtgca
      541 ttcgattttt tatctgatat caaatcttta tgcgaaggaa aagtttctga tactaaagta
      601 gatgtagatg caaagtcaat gacaaaaaaa attaagggtc ttgcaaagca gtatggagct
      661 tctgtagttg gtataactaa attaaaagac tatcatattt atacaaatag gggtagacat
      721 gaagaaaatt acggtgaaga aattaatcta actcataagt atgcaattgt atttggatgt
      781 gagatggata aagaaatgat aaatagagct ccaatgatat gtgaagttat agaaacatca
      841 aagtgctatg tggatgcatc tatcgtcggc atgattttaa gttactatat aagaaattta
      901 ggatatgatg ctagaaatca tatggatgct aattatttgg taatgcctgt ttttatagct
      961 cgtgatgctg gtttaggaga tataggaaga aatgctatac ttacgaataa agattatggc
     1021 tctagactta gattaggtgt tgttactaca gatattcctc ttttagaaga tgaatatgtt
     1081 gattttggac tagaagattt ttgtaaggtc tgtaaaaaat gctcttttaa ctgcccttct
     1141 cattcgcttt ctaatgatat taaaattggt gatgatggaa aatataattg ggtaattgaa
     1201 catgagacat gctatattaa atggagatat ttaggtacag attgtggtat gtgtatatct
     1261 gcttgcccat ttagccaaaa tctcgaaact ataaagaata ctacttcatt taagggtaat
     1321 aatgagctta ttcaaaaagc tttagatgag tatacttcaa aatttggtaa aagaatattt
     1381 atacctggaa atccatcttg gttgaaataa ttttatttaa taaagaatct gtactagaat
     1441 ttatcatagg acaggttctt tattaaatat ataaattttt aaatactttt tctcgtttat
     1501 aacaattcag caatgttttt agtctataat tgacattata aaatattaaa ctaaaaggag
     1561 taactgtatg gaaaaaatat tagtagtaga agatgattct atattaaata aaactctatc
     1621 atataaccta attgaagatg gatatataat tacttcaaaa tttacagcta aatctgcact
     1681 aaagtctatt tttgaatgtg aatttgattt aataattttg gatattaatt taccagataa
     1741 aagtgggttt gaattatgta atgaaataaa ggaaaattac aatataccaa taattttttt
     1801 aactgctaat gatatggaat gtgatatgat taaaggctat gaacttggag ctttggatta
     1861 tataacaaaa ccatttaata tcaatatatt taaacagaaa gttaaagctt ttttaaatca
     1921 tcttacaata aaaacaaaac aagattacta tagggatgga tatcttgaaa ttaacttttc
     1981 tgagttatct gccaatataa atggaaatca aattatattt acaccattag aatatcgtac
     2041 tttaaaactg cttactgaaa atcctaaaag tattttaaca aggaaagtac ttttagaaaa
     2101 actctgggat atagatgcta actttgtaga tgaacatact ttaacatctg ttataagtag
     2161 gattagaagc aaaattgaaa aagacaatct taagtatatc aaaacggtat atggtatggg
     2221 atatatgtgg ttaggtgaaa gaaatgaatt ttaaaaaatt ttcttttagt tctatttatt
     2281 taataattgc tgttggtata atttttacag ctattttatt tagtatctca atttatctaa
     2341 taactgatga tttaaaacta attgctctat tattaatata tacaatagta ctttgtggat
     2401 ttatagcatt aataatattt ttacttcata aaaaaatagt tatattttgt tctaaaatat
     2461 gtcacacttt agatagtatg atgaatagtg aagacaatat tgaaattgaa ttagaagaag
     2521 aaactatttt atcacgtatc aatcacagat taattagaat gtatgaagtt attcaagaaa
     2581 atcgcaatag tattgctata gagaaagctg atttacaaga actagtttct gatatatctc
     2641 atcaaattaa aactcctatt gcaaatttaa aaatgattaa tactactcta ctaaatcaaa
     2701 catttaaaac agaaattcaa aaggattttt taatggatat ggaaaaccaa ttagataaat
     2761 tagatttttt aatgcaatct atggtaaaaa cctccagact agagacaggt ataatcacac
     2821 tatctaaaaa gaaaaattca atttatgaaa caattgctac agcacttagt ggaattttgt
     2881 ttgatgctga aaaaaagaat attgaagtta cagttgattg tgacccaaat ttatatataa
     2941 ttcatgacaa gaaatggact tctgaagcta tttttaatat attagataat gctgtaaaat
     3001 acacaaattc taatggtaag atacgtgtag ctacagaatg ttgggttatg tatacaaaaa
     3061 tagatattat tgataatgga aaaggaatat cagaaagtca ccaagcagaa atctttaaaa
     3121 gattttatcg tgaagaagat gtccatgaca tagaaggtat tggaataggt ctctatcttt
     3181 caagaaaaat tatatctctt caaggtggct atattaaagt tacatcagat attggaaaag
     3241 ggtcgacttt ttctattttc ttacctaata aataaatttt taaatatttc atttgtaatg
     3301 attctgcaac attttggttt tatactgtat ttacaagatg ttaaggaggt attattatga
     3361 gtattttaca agtaattaat ttaaagaaat attatggaca aagttcaaac attacaaaag
     3421 ctcttgatgg tgtaactcta tctattgaaa aaggcgaatt tatttctatt gtaggtacat
     3481 ctggtagtgg caaatccaca ttattaaata tgatgggagg attagataat cctacatctg
     3541 gaaaggttat tataaagggt aaggaattat ctactatgac tgatgaacag cttacaatct
     3601 ttagacgcag aaatataggt tttatatttc aaaattataa tcttgttcct ttacttaatg
     3661 tatatgaaaa tattatctta ccaataggat tgaatggaga gaccatagat aaggatttta
     3721 taaatgaaat tataaatatg ctttctcttg aagaaaaatt atatgatatg ccaagtaatc
     3781 tttctggtgg tcaacagcaa cgtgttgcta tagctagagc acttgtaaca aaaccagcta
     3841 ttatactagc tgatgaacca acaggtaacc ttgatagtag aactagttca gatgtactga
     3901 gtttacttaa agtgacaagt aaaaaattca accaaacaat tgtaatgatt acacacaata
     3961 atgaaattgc acaactaact gatagaatta ttcgtattga agatggaaaa attgttaact
     4021 aggaggtgta gtatatgttt agaaataata atcgcacatt tataagaaaa atagctttaa
     4081 atgatttaaa aattaataaa cttaaaacat atctttcagg tatcattata atgatttcta
     4141 catgtttatt attaacagtg accttagttt catataatgc ctctgtagat ttagttaatg
     4201 catctccata tcatgctata tataaatctg tagatgaaaa agcaaaaaat atactatata
     4261 aaaataaaaa ctttagtggc gtaggaactt acaaattaat tggaagtaat aaaaagacag
     4321 attgtattat gtcgattgtt tatgcagatg atacagcaat taaacttatg aattttcaac
     4381 cattgaaagg caaactacct acaaataaaa atgaaattgc tatttcagaa aaatatttac
     4441 aggaatttgg tttagataaa gatattggag atagcattaa attaaattat tatgatgata
     4501 ttacgaataa agaagttcaa tgtgatttta ttattgttgg atttctagaa aattattatc
     4561 aagataatgc caagcaatac tatacagtcg tttcaaatga ttattttaag aatatagctt
     4621 ctacaccttt acaaaataaa agtaatactt ttgacgagtc aagacctgat actgtagatg
     4681 tattagttaa actatcagaa gaaaatacag agaaagattc agcttcaatt aagacacagt
     4741 taaagaaaat tggtttatct ttaggtatta agaaatataa tatatactta aatgataact
     4801 atattgaatc aaatttaatt gatggtgagc agattattac agttttagta gttggaatag
     4861 ttgttctttt ttctagcgtt tttgttatat atagtatttt ttatatttca gttgttaatt
     4921 cagttcagat gtatgctaag ttgaaatcgt taggtatgac ttcttttcag ttaaaaaaga
     4981 ttatttctct acaaggaaat attttgtcaa ttatttttat tccattaggg gttatagctt
     5041 cttgtataat tgcttacata attcaaccat tagcatggca aatgatagct gatttattta
     5101 taattttaat tttatcactg gtaatgtttt taactgttag aatttcttta tttaaaccag
     5161 ctagaattat ttctaaaata tctgctattg aagccatgca atatacagaa actaaattta
     5221 gaaaaaaagc aaaaaatttt agttatataa atataaaaaa tctagcatta aagaatatcg
     5281 agtcaaatag aaagaaaaat ttgattgcat taatatcgct aagcattagt ggagttttat
     5341 ttatttcaat cgctaatctt gctaattcaa tagatttcaa aaaacaacta tctcaacaat
     5401 ttgtctataa cgaagattat ataattagca ttaagagtga taacttatat gagcatatta
     5461 cagaaatcca acaaaataat ccactaacag attctcttaa gaatgaaatt aaatctattc
     5521 atggagtaaa aaaattaatt gaatccaaat caattaaaag taatattata gaaccttaca
     5581 tcaaagatag agacgaaaaa caatttatta ctctaattaa aggtattacg cctgaacttt
     5641 caaaatcact tgaagaatat attgtcagtg gaaaaataga ctatacacat ttagattcag
     5701 atagtttaat tataaataaa tatagaacaa aatcttatgg attaaattta aaagttggag
     5761 atacagttac atttaatatc tttagtggaa atgatattat taaaaagaaa atgaaaatta
     5821 taggaatcat tgacaattcc aatacaggag gtatgttttt tgcttcaaac aaaactttag
     5881 aatctctaac accatataat acaaatttag atttttctat tttaatagat aaaaagcatt
     5941 ctaaagaagt tgaagaaaag ttgttaagta taattcagaa aaattcaaat ttaatgcttt
     6001 attcatatga agatgattat agaatgatta ctagagcatt tcaatatata attatagctt
     6061 catacatttt tgttggttta atttcttgct ttggtatact aaatatgacc aataccctaa
     6121 taaacagcgt tttaattaga aaaaaagagt ttgccttact tcaagccgtt ggaatgacaa
     6181 gaaagcaact gcgaaatatg ctttatagag aaggtctaaa cataagtatc aaagctatat
     6241 gtacttcaag tatactaggt tattttggtt caaatctact ttgcactttt ataaaagatg
     6301 taattagatt agactttatt aattttaaat tttctatatt tactatttta attttttcat
     6361 tcgtattaat tggtatacaa gttttagtga ctgaattact tgtaagaaat atagaaaaaa
     6421 aatcacttac agaacgctta cgttctcagt aagaatgtat ataaaaaata gccatttttt
     6481 atacaagttt taatatgatt ttttaaaagc tatagggata gaaaattttg actttacata
     6541 attttctatc cctatacttg tttttatttt atacagatac acttacttat atctccgagc
     6601 aacatcacca gagacataaa caagcatatt ccttattttc taaatatgtc aattgcatga
     6661 cttgtaaccc ctactaaatc ttcacgttca caagtagcaa ggtgatattt taaatataat
     6721 gcaaatgcat catcatccat agagtctact gcttcacgca tatacaaagc caaaccatct
     6781 gatgcgacat aatgcagtct agttacatta aatgcagaca tcaaatcatc aatgttttct
     6841 ttacgtacaa gctcaaacaa atcctttggt tcagacttag cagcaaatgt ctgtggatca
     6901 atcaaacctt tttcaatata ttcagataca tcaatatttc ctctatggaa cccctcgtca
     6961 atgagacagc catcagatat aacatatgca gcaaaaacca caccaccagg ttttgttaca
     7021 cgaattgcct catgtaaagc ttgttgttta tcttctttgt tataaagatg atatagtggt
     7081 cctaacagta acgtaatatc atatttatta tctgggaaaa cagataaatc catagcatta
     7141 ccctgagtaa tgcttatatt ttcatttgat tgagtatttt tatggaaaac ctctatatta
     7201 tgttcgacta attccactgc gtcaacatca tatccctgac gtgccagtgt gtgagaataa
     7261 cgaccagttg cagcgccaat ttcaagcaca caatcacctg attttatgta cttatcaatg
     7321 taatgcatag tagtgagaaa ttccaccatt ccatgtttta atgcaagtcg actatcttca
     7381 tcataagtat tataaaagtc aattaagtat tgttttgttt ccataatttc acctcttcta
     7441 tattagttaa taaacatcaa atagacaaat tccaatctgg tttttcttgt cttataaaag
     7501 ctgttaatat atttttcaac attttttcag cactatatct atctaatgtt aatttttttg
     7561 ttgctgttaa gacagctatg ccatgagtat aaagaaataa atctaaaaat attacttttg
     7621 cacattccaa atctattcca atgctttccg aaaaaagtct tgcctttttt tcattgtcaa
     7681 tttctttata aaaatcattc gcctctttca tcatcaaatc taaatcattt ataaataaca
     7741 acttaaataa atacgtttct tcttgagcaa attcaatgta tgaaagcgga agcagtaaat
     7801 aagggcttac acttttagaa ttacgataat taacaacata ctgattataa tattcatatg
     7861 caaaagcaag aaattcattt tttaattcat tcatgttttc gtaacaagta aaaattggtc
     7921 gagtggaaca ctgtaattta cttgcaatac tcctcgcatt tacagcttca aatcccactt
     7981 ctcttgtaat atccaagact gtattcaaaa tcatttcttt tgttatcttt actttaggtg
     8041 gcatatatgt tcacctctcc attaaatttt tataaaacac atatgttata taactatagt
     8101 aacatatcaa gaaattatgt caagaaaaag ctttgcattt ttttgcaatt cctaaaactt
     8161 ataaataaaa catgagttgc aattaatcta ttaatagata acagcaactc agattttcct
     8221 ttaaatactc ttctttttgt ctaatatttc atatacacaa tcttctattg aattaatatc
     8281 tttttctttt ctttttttag aatcaattaa aatgaaatac agtataaaac ctacagcaag
     8341 tccccatgtt ccgcctttag cagcaattac acctcccata agtccaatca atcccttctc
     8401 aacacctgta ttacatagct ccattgatag ctttgtacat aaatatcctt gaactaaaag
     8461 tgttactgac aatgctaatg gtataagagg tgtagcaatc tgcgcaattg gataaaagca
     8521 gataaaaatt gctgaaaccc atactaatgt acccatacca ctaaaaacag actgcatggc
     8581 ttcctttcca accttgtacc tttgagcaac acttcctgta agtgtcgcag ctaatggacc
     8641 acacattggt atatatggag aaaagattgc cataaaaata tttcttatcc cacttataac
     8701 attagaccta tttgaattga aatctatata ttcatctttt ctattttcac tttcagaaag
     8761 tactaaattt tcacctgtta taaaatcacc aaatgcaatt atgtatatca taaaagccat
     8821 tggtatccct tgtataaata acattacact tggaaaacct attccaaaag gagatactgt
     8881 atttatcaga tttttaaaat ctactatgta aactaaattg tcaaaactaa atctaggaat
     8941 ttctatttct ttactaaata tacctactat taaactcact aaaatagctg atactacacc
     9001 atattctcct aaatgaaata aaaatttatt cttaactttt agaatatcaa atctttcaga
     9061 aaacattaca aaacaagcaa ccaaaattcc tactgttata gatataggat aagaatcaaa
     9121 tctcccttta ggattaaact ctgatattat agtcgatata ccgacaccca ttagtattcc
     9181 acctttaata gaatttggta tcttactaac taactttcca gctacaccag ttatacccat
     9241 taaaataaat attattccta aatctatttg taaagctgtt aatgcttgta ttctttcaat
     9301 gcccatgctg aactttaaca aatatgcagt tgtaagagtt atagaagctg taatccagcc
     9361 aggtacaact gggtctccca taaaaacatg caaacagtat aatattgagt taatgatagc
     9421 catactccat gccacttcat agggtaatcc caaaatttgt gtactaaggc caattgtgcc
     9481 taaacttgtc atacttgata ttataccttg aatagcttct ggtttctcaa atctgtaatg
     9541 tataaatgga attcttatct taaacattcc aaattttata tgaggttgtt cccttctagc
     9601 gagtctttcc ataacttcac tccacctttc aataactctt ataagtttac aactttataa
     9661 ttgtttataa attatcaatg tcattaaaat tagtatataa catgcaaatt atataaacaa
     9721 taaattaaat ctataaaatt gatagatttt gtgcatatat agaaagaccg cttcattttt
     9781 agttacttaa atcacttaat attagttatt taaatcactt gataaaggtt ttttataaaa
     9841 ttcaatagtt ctattccatg attcaaactg tccttttgaa tttccttcta cagttccacc
     9901 tgaagaaaaa atccctccac tcatactcat acataaagtc tcctttagag gcataatata
     9961 tggaattggt aacggatgac ccatattatt gtatgttaat aattctacgt tatatggata
    10021 attattttct tcaaagattt ctaatgcctc taaacaagct tcataactat tccatatgtt
    10081 atcttctttt ccagcaatca taagtatatc tgctttggaa ttttctattt ttattctagc
    10141 ctcttttcta ttctctgctc ttttaagact tttcttataa gtactattaa atccaaatgg
    10201 aatatctttt gatatatttt ttttctgttc ttcaaagaat atatcattgt ctactttgat
    10261 atatggaaat ggctctcctt tatatgacca tgatgaagta tcatttccac tcatcaatcc
    10321 attaagagct tggaaacaat aaatgtgagg ttgtgatact acaacctttt taatttgttc
    10381 atatcttgaa gcaagtaaaa aggccaattc tccacccttt gatgtcccat gtacaaatat
    10441 ttctttagta ctggtaattt cattttcact aatccactca aatacttttt caaaatattc
    10501 taatggaacc tcttctaatt tatctggtaa cccttctaaa gcaaagtatg caactgttag
    10561 tacattgaat cctctagatg caagtggagc tgctaaaagg tctagagcgt ctaaatttcc
    10621 atcagaacca cctagcatta aaattgtttt attattagaa ttttgtttat aaaataattt
    10681 tcctataaac tcatcagata tgtattttgt ggtaatatct tctgatttaa atattctctt
    10741 taattcaact tcttttcttt caatagaagt ttcaaatatc atattcataa ttattggttt
    10801 atcaatagat atattttctg caaaattttt accttcagtt ttagattttt tcagcgaata
    10861 aattaacccc atacaattat cagccttata agtaccttct ataggttcaa ctaaatctaa
    10921 atctacttct cctttttcat ttgaacaaaa cactccataa gatgaaaatt cttctccctt
    10981 gcaccatgga aattccattt tcacacttat tttaagttta gtatttggaa ttaagtccga
    11041 aaccataatc ttaagtcttt catccacaaa tgattctaca ttgtatacat ttactttcat
    11101 aatctatttc tccttataat aattatttat gttttataca aaacagcttt atctaataca
    11161 agataaataa atttttaaat taagacacca tattgactat ttcattttat caacatattc
    11221 catctttatc aacatattgt cttttttagt aatttaaaat tatggtctat atgaaatcca
    11281 gttgattttt ggctaattaa agataggaat gctattacgt ttaaaatata tctattcaaa
    11341 ataaaaaacc actcatttta agaatggttt ttaatataaa ctatttgtta ttaataagga
    11401 tattttttta tttaaattta tataatcgaa catctaatat ttaatattta ataaaattat
    11461 gcatttgcaa aagtatatat ggcattaact cttgctacat ataaattctc atcactacat
    11521 ccaatatatc tagcatattt ttcaatcttt tcttttgcat tttcaacatc attttcatcg
    11581 ctctccatta actcatttat ataaatatcc attaatgacg ctttatattc tcttatatca
    11641 tagcctaaaa attcagtcaa tcgctcaatt tccttactaa aatctccatc ttcttttacc
    11701 aaaaattcgt atacatcttc cctatatgga tttaaagtta ttatttgttt tactactttg
    11761 cattcgtcag ccttcccaat aaaaccatct ttataattat taaataaggc attagatttt
    11821 tttatagatt cttcatcaat ataagaatgt accgcagtta tacgattttc tataagggca
    11881 tctacaacag cataatgaat tttaaaacaa tcattgtata aataattgct cattttcttt
    11941 attgtcgatt ctatttctaa gaagctagcg tggccatttt ttttattttt atttggtata
    12001 ataaaagttt tattcaagtt attaaaaagt ctcttaaaat ctaaatattt ttcacaataa
    12061 acacttttaa aggtatcgat atctatagtt ataattccaa aacttactat agtctcaatt
    12121 cctttcttaa aagactcttc tatatatttt tctcctaaac ttaaacctct atcagatatt
    12181 tgctttagat tttgaaaact atttaaacaa tctctctcaa agtttttaga tatttctaaa
    12241 gcatataatt taaagatttt ccttatctta ttataattat tttttgtatc atcaaatctg
    12301 ataatagctc catttacatc aaaagtaatc attgagtttt aacctcccca ttagtttcat
    12361 caataaaata aatttaatat aaactttata atcataatat taaacttatt tatactataa
    12421 ttattaattt gatgataaaa gctattttcc ttctaaataa aaaatttgtc taaagtttct
    12481 aaaaaattta gacaaatctt aggttattac tctttaaatt atttttaaat tattccaaat
    12541 acgccaaata atattggtat aaatatagct ggtaacaaat tggctacttt tatattcgtt
    12601 aatttaagta catttagacc aagtcctata attaataaac ttccagctgc tgtcatttct
    12661 gtgactacag gatcacttaa aaatccattt aaaaatgatg ctcccacaca tataatacct
    12721 tcatacaaaa atacagatat tgcagaaaac attacaccta tacctaaaga agcagtaaat
    12781 ataattgaag ctattccatc tataacagat tttacaaata aagtatcatg acttcctgta
    12841 agaccacttt ctaatgaacc aactactgcc atggctccta tacagaataa taaactagat
    12901 gatataaaac cctctgctat agaatctttt ttatttcctt tagaaaattt actttgtaaa
    12961 aaagcaccca acttatttac ccatttgtca atatctatta attctcctat cagagcacct
    13021 acagctacag atattattgt cactaatgtg ttttttcctt ctagtgctcc tgatatacca
    13081 atgtacaata cacataaagc aattccattc attatagtat cgcttactct ttgaggtaat
    13141 cctcctttta ttataagacc tactatacat ccacctgcaa ttgctagact atttgctatt
    13201 actccaaaca ttttttctcc cctcgattag ctaattgaaa tttatcctat tattaaaacc
    13261 taaacatcta tactttgtat tggatatttt aaatattcca aaaattcttc aaaacctttt
    13321 ctagatatat ttacagtttt agtatttatg ttaggatgaa aatttaagta atcttcttta
    13381 attacatctt tatctatgta aagctcaact tttctttctg tatcatttat taatgaaaaa
    13441 ggatttacac ttccaggaag aagtttaagt acattgtata gtctttcagg tgaagcaaac
    13501 gataatctag aacttcctat cttatcttta agtgagttta aatcaacttg tttttctcct
    13561 ttaactacaa taagataata cttatttcct ttggcatttc ttaaaaataa atttttacat
    13621 tgacacccct ttgttattcc atctaaaatg actaattgct ctgctgtata tacagcttca
    13681 tgctctacta catcatattc aacatttaat ttatccaaaa tatcatatat tttagtttca
    13741 ttcatgctag attcattttc atattgttct tttttattat ttaaatcact catacttttc
    13801 tccttaaaca cttcatcata atatcattgt gaggtattat tctactatag ctattgctct
    13861 tactggagct ccatcagcat cattatactt aaatggtgct gctataaata gaaatttttc
    13921 tggaacattt tctaaatttg taagattttc tatgattatt tttccttttt caaataaaat
    13981 atgatgcact tcaaatactg atgtgtcata tctatccact gatagcatat ctattcctat
    14041 gccttttata ttagtattag ctatatagtt tgcagcttct tttgttaatg taggataacc
    14101 tacatagtac tgctttttat cccaaaattt tgaccaacct gatttaaata ttataaaatc
    14161 actatttttt atttcatctt catatttttt tagatattct aattctatat tctcatttcc
    14221 cttttcaagt tccaatacat aagctttccc aacaaaattt tctatatcca acttatctag
    14281 ggtctcacct ttagtataca tatgtcttgg tgaatccata tgagttccat tgtgagaaaa
    14341 tacactaatt aatgtttctt gataaccatt ttcttcaata atagctactt tcttgatatc
    14401 tggtctatta ggctctgcat atacaggcat gtcattatgt gtcacatgag ttaaatcaaa
    14461 aaccttcatc tttatatccc ccctatgttt ttattttcta ttatatacct aaattcattt
    14521 tatttctatc agattttatt ccattatata ccatactact atatattttt tataaacctc
    14581 aagggattta ccgaaaaatc ttggaaagaa ttatcagtaa atataacatc ttcacccaaa
    14641 aaacgtttat accttccttc ttctttcttt aaagtaattc tctgtttttg tgaatgatta
    14701 ttaaaaacca ttatcttaag aggaggtttt tttctattag tctgcactct ttttaacatg
    14761 tattttatgt gcatatcagc ttcaggaaat gaatatccac aaaatattaa aaggtcagta
    14821 tctcttaaag ttttttctgt ttcattccaa acattactta aaaatatgtt tgacatattc
    14881 ttaaaatatg ttggtggcac tataataggt tctgttaact caccacactc taaacatttt
    14941 gtctctgaac tattttcaat aagcttcatg acgccacctt catgtggtgt aagagttaca
    15001 ctattacaaa tggggcaata aagccaattc aatgacccat gtatcttata taatttgatt
    15061 aatggttctt ttggttttct ccaatttaac tcctctttaa aatttgtaaa ctccacacca
    15121 taatcaagca taattggcaa atcattctta tctcttaatc cagtaactgt attgtctata
    15181 tgtatatcat aatttgcact aataaaagta gtatcaagaa gcaaatcatt ttccaaaaga
    15241 ttttcaacta aaagtttatg atatttatta tctgttctag gtgtattatg aattgcttca
    15301 gccattagca gtattaaata ttgtcttaaa aaacgtatac tatcacttct gttatttaga
    15361 ttttctagtc caaaatttct aaatgcttct tttctttgtt ctgctaagtc taaaagacct
    15421 aaaacttctt caaaggtagg aaaatttgca ttatctatat tatcttttac tatatcaata
    15481 ttgaacatct gtttaaagaa aatatacaat tctctattca ttttggtatt ataatttgtg
    15541 ggatttagtt ttttaaaata ttcgctaaaa agttcatttt gaataggtag attctctgct
    15601 gctgaagctc ctgctcctaa aaatattgct attttcatat agttcctcct aatataaata
    15661 ttactcctat ttttatatcc attaaatatc catatcattt attttatgat acaacaataa
    15721 agctgtcttt tactagaaaa aatcctatta caagacagcc ttacattata ttaaatcttt
    15781 aataattcgt aaatagaaag agcatatatt tgagtagctt ttataatatc ctctactatt
    15841 aagtattcat tagcttggtg ttctgtttct ggttttcctg gaaaaatagc tccaaatgca
    15901 acacaattat ctaaagctct tgcgtatgtt gctcctccag atgaaagtgg tgttccatca
    15961 agaccagttt cttcttcata tacttttcta agtgtcttaa caagaagagt atcttctgga
    16021 acatagattg aatccaagaa atcatattct tcataattta aattgtattt atctgtcatt
    16081 ttctttaact cttttacaaa atcatccttt ttgtatgtta ctggaatcct tacatctatt
    16141 cctgcaaatt ctttttcgtt atcaatagtt actctaccta tatttactgt tagtttacct
    16201 gatacatcat ccttgcaatt aggaattata ttgtttccat tagcatcttc accaataact
    16261 tctgctaaaa acttaattat attagaatct atacctatat tattaagagc tatacaaagt
    16321 ctagccactg catttatacc agtatcactt gctgctgaat ggacactttt tccaattata
    16381 cagattttat taccttcaac agtatactca aaatttaatt tatcaagttc tttttttaat
    16441 ttatcagagt attttccaat atagattgct ttacctggaa ctgcatttaa agcctttcca
    16501 actgacaact ctatatcact tttgctatca caagtcaaat gaacttgtaa aagacctttc
    16561 tctgcatttg ttattgggaa tctagaatct ggagtaaatc catagtttgg tatttcttca
    16621 ttattttctt tatatttatt tatacatctc caaagatttt cttcatcagc cccaaaaatg
    16681 aatcttatcc tcttattaaa gtctacattt aaatccataa gagctttcac tgcataaatt
    16741 gctgatattg taggtccttt atcatcttgt gttcctcttc catacaattt accatcttct
    16801 aaaacagctt caaatggagg gtaattccag ctttctaaat caccttctgg aaccacatca
    16861 acgtgaccaa gtatacctat cattttttca ccttgtccta tttctgcata tccataatat
    16921 ccatctttgt atactgtctt aaatcctaaa gatttacata agtctaatat acctcttaaa
    16981 gctttatcta cattttcacc aaatggacaa ttttcagttg cttcagatat aacactaggt
    17041 atttttacac tttcttgtat tgaagaaatc atttcatctt gtagagaatt tactttttct
    17101 ttaatttgct ctttcatatt atcacccttc aattttcatc ataatatact aataaatatt
    17161 ttgacctttt aaaaaaaaaa tatgcttatt ttttcagttt ttttataaat tcaatcgctt
    17221 ttaaagtccc catttttgaa tggtcaaatg taagtccacc ttgaaaataa acattataag
    17281 gaggtcttat tggagcatct gcacttaatt ctatagaaga accttgtata aatgctcctg
    17341 ctgccattat gacttcactt tcgtatccag gcatgtccca aggaacaggt tttacataag
    17401 aatcgactgg tgcagcttct tgtatacctt cacaaaaact tattacttca tcagcattat
    17461 taagtctaat acattgaata atatcacttc ttaagtcatc atattttggt aatacatcat
    17521 atcctaattt ttcaaaagct cttgagcaaa agatagctcc cattacagcc tgagatacta
    17581 tataaggcgc caagaaaaaa ccctgaagta catttctagt tgttccaaaa gtaagaccac
    17641 attcttttcc tattccagga gaagtcattc tataagatat aagctcaatc aaatcttttc
    17701 taccagctat atatccacct gtcaatgcaa gcccaccacc aggatttttt attaaagagc
    17761 cagccataac atctgctcca atatcagtag gctctttggt atctaaaaac tcaccataac
    17821 aattatcaac cattactata gcttctggtt taacagactt tattacttct atagcttctt
    17881 ttatatcact aattgaaagt gattttctcc aagaatatcc ttttgacctt tgtatcataa
    17941 cgagtttagt tctatcattt attttgtttt taattccttc taaatctaag tttccatcct
    18001 ctaaaaaatc tacatcatca tatgtaacac catactcttt caatgaacct ttttcttctc
    18061 ttatgcctat aactccctct aaagtatcat atggtctacc agtaacagat aaaatttcat
    18121 ctcctggtct aactatacct tgtatacata aagtaagtgc atgtgtacca tttacaatta
    18181 ttggtcttac aagagcatct tctgtattaa atactttaga ataaatttcc tctatttttt
    18241 cacgaccaat atcattatat ccatatcctg tagtccagtt aaagtgcata tcacttagtt
    18301 tagattcttg catagctttt aaaactttat attgattata ttctcttatt tcttttattt
    18361 cttcaaactt atcttttatt tcttccatta tttcttgcga tagtttaaaa gtatcatcat
    18421 ctattccata gtagtctttt aataattctt ttgtctcatt aagcatttta tcacctgtct
    18481 ctttattatt ttatttaaac atgtaaagga gactatttta ctaaaacaaa taaattatag
    18541 tttataagtt agtataagtc tcctctcaat catcttaaat taaattaatt atctatctgt
    18601 tgttattatt attgttgttt tggttttgat ttgttagatt aatatacttt ccaggttgta
    18661 tagttgatac agcatgttta taaatcatgt tttgttgtct attatcccct tctattaata
    18721 ttatataact atcaaacccc tttacaagcc ctttaacttg cactccatta actaaatata
    18781 tagtaacagg tatcctttct ttccttgcat tatttaaaaa caaatcttgt aaatttaaaa
    18841 ctgtattttt cattagattt gtaccctccc aagattaaat tgaacttcta aaa
//
LOCUS       DAEMHW010000064        18889 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEMHW010000064 DAEMHW010000000
VERSION     DAEMHW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.064"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            75..1676
                     /locus_tag="KPK67_001880"
     CDS             75..1676
                     /locus_tag="KPK67_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBG2458849.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCISASKSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDKNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSDFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGGL"
     gene            1895..3484
                     /locus_tag="KPK67_001881"
     CDS             1895..3484
                     /locus_tag="KPK67_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HBG2458850.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGSPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNINTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYNLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVIDGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDARELANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            3697..5850
                     /gene="slpA"
                     /locus_tag="KPK67_001882"
     CDS             3697..5850
                     /gene="slpA"
                     /locus_tag="KPK67_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBG2458851.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFAAVQTDDAGHTVSQKDYKKAV
                     KQIQDGIKSNVITEVKIYFDGSEVKTLSVTHGSEDADVKAREQEATDFYNAVDGKLDG
                     LGDGKYVDFEIKYNDSLEVYSKSELTTYKDLLDNKIVAKKAVSPSTAGIVISNASTAD
                     NADPAANDLKGSDIYDIQVVPDDTNGGYKLTLTAKDISAYKYGTPGTDGFKNGTALVA
                     STTPDELVLQKGKKLNITDSLAFTSGNGAISGIKTEATSPEEKTVKVRIINVKEITVD
                     LDASSYDSAESLAKKYVFKPSTLTSIYTDIEAGNIDRVNGKYQVEIFADGKRLNTLSA
                     VNSTIADPDSTAKVIIKADKLKDLKDYVDDLKTYNNGYSNSTNVSGDDRIETAIALSE
                     KYYNSDDDNALFEKEVNSVVLVGSNAIVDGLVASPLAAEKKAPLLLTSKDKLDSNVKS
                     EIKRVMNLKNTVGVNSSKKVYLAGGVNSISKDVENELKDMGLKVTRLAGDDRYATSLE
                     IADEIGLDNDKAFVVGGTGLADAMSIAPVASKLESGEATPIVVVDGKAKTLSSDASDF
                     LGNAQVDIIGGENSVSKDVERDIDDATGKSPERVSGDDRQATNAEVIKNSDYYEKGKV
                     KNFFLAKDGSTKEDQLVDALAAAAVAGNFGTKFDVAGGETSPAPIVLATDSLSSDQSV
                     AISKALGDNDGENLVQVGKGIATSVVTKLKDLLNM"
     gene            6114..8459
                     /gene="secA"
                     /locus_tag="KPK67_001883"
     CDS             6114..8459
                     /gene="secA"
                     /locus_tag="KPK67_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG2458852.1"
                     /translation="MSVIDSILDKADEQEIKRLNVIVDKIDALEDSMKNLSDEELKDM
                     TAIFKDRLEKGETLDDILPEAFAVVREVSKRKLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYESLDMTVGVIISNQD
                     PSIRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGDGDEDLKLYELANSFVKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEIISKAGKLNAITIATNMAGRGTDISLGAGDKEEEQEVKDL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMLGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            8873..10744
                     /locus_tag="KPK67_001884"
     CDS             8873..10744
                     /locus_tag="KPK67_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBG2458853.1"
                     /translation="MNKKNLSVIMAAAMISTSVASVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSRTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYIDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHTTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            10851..11558
                     /locus_tag="KPK67_001885"
     CDS             10851..11558
                     /locus_tag="KPK67_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG2458854.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSENEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELATNKIQYVHAP"
     gene            11591..13735
                     /locus_tag="KPK67_001886"
     CDS             11591..13735
                     /locus_tag="KPK67_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG2458855.1"
                     /translation="MKISKKIVSLLTITFLTIMLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWTKSESAVLVNDNSIADALSATPFAKAKNAPILLTQSNKLDDRTKVELKRLGV
                     KNVYLIGGTNTLDINVEKQLKAENISFERISGNNRYQTSLKLAEKLSKEKEISNIVVV
                     NGEKGLADAVSVGSVAAQENMPIILSDTINGIKDTEKFIKEKNIKKSYIVGGIYSVSN
                     SVEQSLPNSKRIAGNNRNETNAKVIEEFYKNTDLKNVYITKDGMKKQSDLIDSLAVGV
                     LAAKNNSPVVLVGNKLDFTQNDVLNTKIIKNVVQVGGLGNENAVESIIDSQEETTYTV
                     KNTDELNVAIKKSDANDIIKFKPEKEKNITESFKVETDKSISVEFEGTYTQTISINMP
                     NGSITNLGDVNGTIKIMDVRSSTLVNKGEINYLDIYDNNGCRVENERDGDIWLVTISN
                     ESRDVHIENDGDINKISNSCSSVTIKNSGNIDTLSGSKEIAISGKKPKVDDTDKDDER
                     ASGINIKVEPCSIPKRDYVKVRIVTEPKNSNYKIYYKVVSKKPTSRYVGDKIKVSEWD
                     DAPSAGTSFEVEARNGFYIEAVEINTSTNKISKWGRSDSTDDGFKSIEVAKGLSANVF
                     IDGDKVELTTTRANSGCEIYYRIMSSKPTAMNVGSSITLSSWEKISSSSLELESSEVS
                     EKYIELVELEKSTNLVTRWGSTEKINIPNSSI"
     gene            complement(13771..14169)
                     /locus_tag="KPK67_001887"
     CDS             complement(13771..14169)
                     /locus_tag="KPK67_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBG2458856.1"
                     /translation="MIFKKHKETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIEFLFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDIIVKVFTNIVVIVLNYLFSKMIIFKEK"
     gene            14397..16808
                     /locus_tag="KPK67_001888"
     CDS             14397..16808
                     /locus_tag="KPK67_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBG2458857.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMDISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            17105..18682
                     /locus_tag="KPK67_001889"
     CDS             17105..18682
                     /locus_tag="KPK67_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBG2458858.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKNLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT         7826 a         2091 c         3490 g         5482 t
ORIGIN      
        1 tatggaaaat aaagagaaaa taaatggaaa ataatcacaa tataaatata aaatataaaa
       61 atcatcaggg ggatatgaaa atgaacaaaa aaatattatc attaggtcta gcagtatcat
      121 taatcttagt aaacttcaaa agtgtaaatg catcatcagt agtagaaaaa atatacggta
      181 aagatagata tgaaacagca gcaaagatag ctgataaaca gacttatgaa acagtaattt
      241 tagtaaatac agaaaaatca cttgcagatg gattaagtgc aagtggatta tcaggagcta
      301 caaaagctcc aatactattt acacaacaaa ataagatacc agcagacaca aatagatgtc
      361 taaaaaatat caaaaaagca tatataatag gtacagaaga tactataagt aaatcagtag
      421 aaaaagaact agattctaaa aatatagaag tcaaaagaat tggtggagaa gatagactaa
      481 aaacaagcta tttaatagct aaagaaatag caactataaa aaaagtggat aaagtactat
      541 taactaatgc atatagtgga gaagcagatg caatgagtgt atcatcagta gctactagag
      601 atggagctcc aatcatactt acagatggaa agagtgttcc ttttgatgta aaaaatattc
      661 aatcatattg tataggttca gaggagataa tgagtaatcc tctagttaaa aatacaaact
      721 cagtaagaat agaaggaact gaccgatttg aaactaacaa aaacgtaata gactactttt
      781 ttaatagtgc agatggattc tatgtatcag atggatacca attagtagat gcaatagcag
      841 ctgcaccact tactaaaaac tctccaatgg tactagtaaa tgatggaagc gataaaactg
      901 tattagaagg agctaagaat ataacctctg taggtgaaat aaatgagaag gtaatacaac
      961 agtgtataag tgcttcaaag tcaaatggac aaccaccaac aattacagtt ggaagtacag
     1021 aggtatataa gggtgaaaag tttgacactg gcaagttaaa tatagtagct aaagataaca
     1081 cagggaaggt attaccaata gaagttgatg ggtttataga taaaaataga gtaggtacat
     1141 acatattgac attaaaagct acagacgaat ggggaaaaag tacaggaaaa agagtagaaa
     1201 taaaagtgtt ggatgacaaa tctcatgatt acaacagccc agaatttaaa aaaatggtat
     1261 ctactgaaat gtataactta atcaattcct atagaaaaga aaaaggcaaa gagcctttgg
     1321 tagtatctag taggctagaa ggtatggcaa atgcatggtc taagtatatg atggataaaa
     1381 aagtatttgc acattatata gatggaaaaa atgctccaca agtattttct gattttggaa
     1441 tgagaagtga ggaaaatata gcatatattt atatagattc taagaacgtt caaacgacac
     1501 aagatgcaaa agacttggca aaggctatat ttgaggtgtg gaagaagtct ccagagtaca
     1561 atgcaaacat gcttagtgat gagttctata gtactggatt tgggctttat atattatcgg
     1621 atggtcaagt gcatgctact caagagtttt taaatggcaa tgaaggtggc ttgtaaaaaa
     1681 ttccataaat cttgtttttt tgttaattta ctgatattat aaaatgaaaa tcacatctaa
     1741 agtacttttt gtaatctgtt ttgttattgc tttacaggtt atggtatttt aggtgttttt
     1801 tgttttagta gtttatagat tttgagggaa tcactactga ggatgtaatt tatgattagc
     1861 tgaattatat aaaaaaatta gtaggaggag tttgatgaag gtaaataaga gagtattatc
     1921 aataggattg acaatatcat taataatggc aggagcacca aatatcaatg cactttcaag
     1981 tatagaaaag atacaaggga aagatagata tgagacagct gccaaaattg cacagaaaca
     2041 aacatatgaa aatgtagtat tagtaaatac agataataca ctagctgatg gattaagtgc
     2101 aagtggatta gcaggaacag ttaaagcacc tatattatta tcacaaagaa acagtatacc
     2161 atcagacaca gaaaaaatgt taaaagatgt aaagaaggtt tatataatag gaactgaaga
     2221 ttcaatagga aaatcagttg aaaatgaatt aaagcaaaaa ggaatagaag ttaaaagaat
     2281 aggtggtaat gatagaattg aaacaagtta tcttatagca aaagaaatag cttcaataaa
     2341 acctatagat aaggttttta taactaatgg ttatacaggt gaagcagatg ctatgagtgc
     2401 gtcatcagta gcatcaagag atggatcacc tataatttta actaatggta aaaatgttcc
     2461 atttgagaaa aaagaaggtg tgcaatgtta tgctcttggt tcagaggaga taataagtaa
     2521 tgatttagtt aaaaaaacta atgcagttag attagctgga gaagatagat ttgagactaa
     2581 taaaaaagtt ataaaacatt tttatagttc agcaaaagaa ttttatcttt ctaaaggtta
     2641 tcaattagta gatgcagttg cgggttcttc tatagcaaaa aatgcaccta ttgtattggt
     2701 tgatggaaat agtgataagt ctgttcttag aagtgctgat aaaatcacag cactaggagg
     2761 aatagatgaa aagacactag aacaatgttt aagtgcatca tcactggatg caagtgcacc
     2821 aacaataact gttggaaatt taaatattta tcaaggtgat aagtttgata taagtaaact
     2881 gaatatcgta gcaaaggata gcaatggtaa cgatttgaca ccagagctta taggaaatat
     2941 taatactgat aaggttggta aatacaaagt tactataaaa gctactgata ttggtggaaa
     3001 gactacatca ataagtgtag aagtaaatgt acttgaatat aagactaatg atatgaatag
     3061 tagtgagttt aaaagaatgg tttctagtga aatgtataat ttggtaaatt catatagaaa
     3121 agaaaaaggg aaagagccat tacaagtttc taaaaactta gaagggcttt ctaattcctg
     3181 gtctaagtac atggcagata aaggccaatt ttcacatgta atagatggta aaaaatctgt
     3241 tgaagtattt actggatatg ggttgagaag tgaagaaaat atagcttttg taccacttgt
     3301 tacaaagagc acttatacga caaaagatgc tagagaacta gcaaatgtaa tatttacagt
     3361 ttggaaaaaa tcggataaat ataatgaaaa tatgctaaac agtgattttg tatatactgg
     3421 gtttggattg tatatattgc ctaatggaga agtgtatgct actcaagaat ttttaaataa
     3481 gtaaaattaa attttttaag ttattaaata ttgaaattta agggtataaa aactcatatg
     3541 cttgggaaaa ataataattt caaaaaaatg ccttgcaaaa agaattaaaa tggattatta
     3601 tagagatgtg agaaatatta ggaatatatg gatgattatt ctatgtacat aataaagaga
     3661 tgtaatttta atataatgtt gggaggaatt taagaaatga ataagaaaaa tatagcaata
     3721 gctatgtcag gattaacagt gttagcttcg gcagcaccag tgtttgctgc tgtacaaaca
     3781 gatgatgcag ggcacacagt atcacaaaag gattataaga aagcagtaaa acaaatacaa
     3841 gatggaataa aaagtaatgt tataacagag gttaagatat attttgatgg aagtgaagtt
     3901 aagactttat ctgttacaca tggttctgag gatgcagatg taaaagctag agaacaagaa
     3961 gctacagatt tttataatgc agtagatggt aagttagatg gtttaggtga tgggaaatat
     4021 gttgattttg agataaaata caatgattct cttgaagttt attcaaaatc tgaattaact
     4081 acttataagg atttattaga taataaaata gtagctaaaa aagcagtttc acctagtaca
     4141 gctggtatag taatatcaaa tgcttctact gcagataatg cagacccagc tgctaatgat
     4201 cttaaaggat ctgatatata tgatattcaa gtggttccag atgatactaa tggtggttat
     4261 aagcttactt taacagcaaa agatataagt gcttataaat atggaactcc tggtactgat
     4321 ggatttaaaa atggtacagc attagttgct tcaactacac cagatgagtt agtattacaa
     4381 aaaggaaaaa aattaaatat tacagattca ttagcattta ctagtggaaa tggagcaatc
     4441 agtggaatta aaactgaagc tacttctcct gaagaaaaaa cagtgaaagt aagaattata
     4501 aatgtaaaag aaataacagt agatcttgat gcaagttcat atgattctgc tgaatcttta
     4561 gctaaaaaat atgtatttaa gccaagtaca ttaacttcaa tatatactga tatagaggca
     4621 ggaaatatag atagagttaa tggtaagtat caagttgaaa tatttgcaga tggaaagaga
     4681 ttaaatactc tatcagctgt aaattcaaca atagcagatc cagattctac agctaaggta
     4741 ataattaaag ctgacaaatt aaaagattta aaagattatg tagatgattt aaaaacttat
     4801 aataatggat actcaaattc aacaaatgtt tcaggtgatg atagaataga aacagcaata
     4861 gcattaagtg aaaaatacta caattcagat gatgataatg cattatttga aaaagaagta
     4921 aatagtgtag ttttagttgg ttcaaatgct atagttgatg gtcttgtagc atctccttta
     4981 gcagcagaaa agaaagctcc tttattatta acatcaaaag ataaattaga tagtaatgtt
     5041 aagtctgaga taaaaagagt tatgaacttg aaaaacacag ttggtgtaaa ctctagtaaa
     5101 aaagtttact tagctggtgg tgtaaattct atatctaagg atgttgaaaa tgaattaaaa
     5161 gatatgggac ttaaagttac tagattagct ggtgatgata gatatgcaac ttcattagaa
     5221 attgctgatg aaataggttt agataatgat aaagcttttg ttgttggtgg aacaggttta
     5281 gcagatgcta tgagtatagc accagttgct tctaaattag aaagtggtga agctactcct
     5341 atagtagttg ttgatggaaa agctaaaact ttaagttcag atgcttcaga tttcttagga
     5401 aatgctcaag ttgatataat aggaggagaa aatagtgtat ctaaagatgt tgaaagagac
     5461 atagatgacg ctactggaaa atctcctgag agagtaagtg gtgatgatag acaagctact
     5521 aatgctgagg ttatcaagaa ctctgactat tatgaaaaag gcaaagttaa gaatttcttc
     5581 ttagctaaag atggttctac taaagaagat caattagttg atgctttagc tgctgcagct
     5641 gtagcaggaa actttggaac taaatttgat gttgctggtg gtgaaactag tccagctcca
     5701 atagtattag ctacagattc attatcttct gatcaaagtg tagcaataag taaagcactt
     5761 ggtgataatg atggtgaaaa tttagttcaa gttggtaaag gtatagcaac ttcagttgta
     5821 actaagctta aagatttatt aaatatgtaa tcaaaatttt tataataaaa aggctcttca
     5881 ttgaagagtc ttttttatag ttataatatt tatcaaataa aaaactctta acattagttt
     5941 taataattgt taagagtttt ttatttgtac caattaatat gtgagaaata cttaaataaa
     6001 aagatgtttg attatttaag taaaatcatt atactataat ttaaaataat tgtctactat
     6061 tatggatttt taaggtataa tagtaattgg acaagagaaa aggaggtact tatatgtcag
     6121 ttatagattc gatacttgat aaagcagatg aacaagaaat aaagagattg aatgttatag
     6181 tagataaaat agatgcacta gaagatagta tgaaaaatct atcagatgaa gaactaaaag
     6241 atatgacagc tatatttaaa gatagacttg aaaaaggtga aactttagat gatatacttc
     6301 cagaagcatt tgctgtagta agagaagtat caaaaagaaa attaggaatg cgtcagtata
     6361 gagtacaatt aattggtggt atagtaatac atcaaggaaa aatagcagag atgaaaactg
     6421 gtgaaggtaa aactctagtt gaagtagcac cagtatattt aaatgccctt acaggtaagg
     6481 gtgtacatgt aatcacagta aatgactatc tggcagaacg tgataaggag cttatgcgtc
     6541 cagtttatga atctcttgat atgacggtag gagtaattat atctaaccaa gacccaagta
     6601 taagaaaaga acaatataaa tgtgatataa cttatggaac aaatagtgaa tttggatttg
     6661 actatttaag agataatatg gtgccagatt tatctcataa agtacaaaga gaactaaatt
     6721 ttgctatagt agatgaagtt gactcaatat taatagatga agctagaacc ccgcttatta
     6781 ttgcaggaga tggtgatgaa gatttaaaac tttatgagtt agcaaatagc tttgtaaaaa
     6841 ctgttaaaga agaagacttt gagatggaca gaaaagataa aactatagca ttaacagcaa
     6901 gtggtataag taaagctgag tcattttttg gtataacaaa ccttactgat ataaagaaca
     6961 tagaattata tcatcatata aatcaagctt taagaggtca caaacttatg gaaaaagatg
     7021 ttgactatgt tatttcaaat ggagaagtaa tgatagttga cgaatttaca ggaagagtaa
     7081 tggatggtag aagatataca gatggacttc accaagctat agaagccaaa gaaggtgttg
     7141 agataaagaa tgaatctaaa actatggcta ctgtgactta tcaaaatttc ttcagactat
     7201 atgaaaaact ttctggtatg actggtactg caaagacaga agaaggagaa tttgagtcaa
     7261 tctataaatt aaatgttgtc caaataccaa ctaataaacc agtgattaga gctgatttac
     7321 atgataaggt atttaaaaca gaagaagaaa agtatagtgc tgttgtagaa gaaataataa
     7381 ggatacataa gactagacag ccaatacttg taggaacagt ttctgttgaa aaatctgaga
     7441 agctgtctaa aatgcttaaa aaacaaggta ttaagcatca agtattaaat gctaaacaac
     7501 atgataaaga ggcagagata atttctaaag ctggtaaatt aaatgctata acgattgcta
     7561 caaatatggc aggtagagga acagatattt ctctaggtgc aggagataaa gaagaagaac
     7621 aagaagttaa agatttaggt ggactttatg ttataggaac agaaagacat gaatcaagaa
     7681 gaattgataa tcagcttaga gggcgttctg gtcgtcaagg agacccaggt acatcaagat
     7741 tctttgtaag tcttgaagat gatgtaataa agctttatgg tggaaaaact atagagaaac
     7801 ttatgaagag aacaagttca aaggaaaata cggctattga aagtaaagca cttacaagag
     7861 ctatagaaag agcgcaaaaa ggtgtagaag gtaaaaattt tgaaataaga aaaaatgttc
     7921 ttaaatatga tgatactatt aatgagcaaa gaaaagttat atataatgaa agaaataaag
     7981 tgttaaatga tgaagatata caagaagata ttcaaaaaat ggttaaagac atcatacaag
     8041 aagcaggaga aacttattta attggaagaa aaagagatta ttatggatat tttaaacatt
     8101 tatatagtac atttatgcca gcagatacat tactaatacc tggtgtagat aaaaaaagtg
     8161 tccaagagat aattgatagc acatatgaaa tttcaaaaag agtttatgac ttgaaaaaga
     8221 tgatgcttgg tattgataag gttgcagagt tagaaaaaac agtactttta aaagtggttg
     8281 atcaatactg gatagatcat atagatgcta tggaacagtt aagacagtat ataggtctta
     8341 aatcttatgc tcaaaaagac ccatttaagg aatatgcttt agaaggatat gacatgtttg
     8401 aagctttaaa taaaaatata agggaagcaa cagtgcaata cttatataaa tttaactaaa
     8461 aaagttcatg taaattttat taaatgaaaa ataaaatatt ttgattcttt atattgtaat
     8521 tacaattaaa taattaaaaa atacaaatga aaaagaagct gaaaatcagc ttctttttct
     8581 ttgtgagaaa tacggaaaaa agagatgctt gaaatttctt tcttccattt aatattatac
     8641 tataaagaag atgaaaagtc tattagttat aaactggatt attaaaaaaa attacaattt
     8701 tgttactttt ttgtatacat aaatattaaa aaataaaata taagaaaata tttatatctt
     8761 ttggttaatt attacaataa gtctcattta ttgaaataat atcaaatata tattataatt
     8821 ggtaaataag gaaaaaataa taaaaatttg aattttttag ggggaaaata ccatgaataa
     8881 aaaaaatctt tctgtaatta tggctgctgc aatgataagt acatcagtag cttcagtttt
     8941 tgctgcagaa actacacagg taaaaaaaga aacaataact aagaaagagg ctacagaatt
     9001 agtttcgaaa gttagagatt taatgtctca aaagtataca ggaggttctc aagttggaca
     9061 accaatatat gaaataaaag ttggtgagac tttatcaaaa ttaaaaataa taactaatat
     9121 agatgaatta gaaaaattag taaatgcttt gggagaaaat aaagaactta ttgtaactat
     9181 aacagataaa gggcatataa caaatagtgc aaatgaagta gttgcagaag caactgaaaa
     9241 atatgaaaat tcagcagacc tttccgctga agctaattct ataacagaaa aagctaaaac
     9301 tgaaaccaat ggaatttata aagttgcaga tgtaaaagct tcatatgata gtgctaaaga
     9361 taagttagtt ataactttaa gagataaaac agaaacagta acttctagaa ctatatatgt
     9421 aggtattggt gatgaaaaag ttgatttaac agcaaatcca gttgattcaa ctggaacaaa
     9481 tttagaccct tctgcagaag gatttagagt aaataaaatc gataaactag gtgtagcagg
     9541 agctaaaaat attgatgatg tccaattggc cgaaataact ataaaaaata gtgacctaaa
     9601 tacagtttca ccacaagatt tatatgatgg atatagattg actgttaaag gtaatatggt
     9661 agcaaatggt acatcaaagt caattagtga tatttcagca aaagattcag aaacaggaaa
     9721 atataaattt actattaagt atattgatgc atctggaaaa gcaacagagc ttactgtaga
     9781 gagtactaat gaaaaggatt taaaagatgc caaagctgca ttagaaggta attcaaaggt
     9841 taaattgata gctggagatg atagatatgc aactgcagtg gctatagcaa aacaaacaaa
     9901 atatactgac aatatagtta tagttaattc aaataaacta gttgatggat tagcagctac
     9961 accacttgct caatctaaaa aagcacctat attattagca tctgataatg aaataccaaa
    10021 agtaacttta gattatataa aagatataat taagaaaagc ccatcagcta aaatatatat
    10081 agtaggagga gaatcagcag tatcaaatac agctaaaaag caattagaat cagtaactaa
    10141 gaatgttgaa agactagctg gagatgatag acatacgact tctgtagcag tagcaaaagc
    10201 tatggggtct tttaaagatg catttgtagt aggtgcgaaa ggggaggctg atgctatgag
    10261 tatagctgct aaagctgctg aacttaaggc tcctataata gtaaatggtt ggaatgatct
    10321 ttcagcagac gctatcaaat tgatggatgg aaaggagatt ggtatagttg gtggttctaa
    10381 caatgtatct agtcaaattg aaaatcaact tgccgatatt gataaagata gaaaagttca
    10441 aagagttgaa ggagaaacaa gacacgatac taatgctaaa gttatagaaa catattatgg
    10501 caaattagat aaactatata tagcaaaaga tggatatgga aataatggta tgctagtaga
    10561 tgcattggca gcaggacctc tagcagcagg taaaggtcca atacttctag ctaaaactga
    10621 tataacagac tcacaaaaga atgcacttag taaaaaatta aatcttggtg cagaagtaac
    10681 tcaaataggt aatggagttg aattgacagt aatacaaaag atagctaaaa tactaggttg
    10741 gtaataattt atactgtgga gaagattcct taattaatac aggaatcttt ttctataatt
    10801 atgctattta ttgtaaatta taacatttta taaagtagga ggatgtagta gtggtagatg
    10861 caactttatt ttttacacca catcaagatg atgaaacctt aagtatggga agtgctataa
    10921 ttgaacatgt tgaaaaaagt gatacacatg tcatattatg cacagatggt agtaaatcta
    10981 taataagaaa agttttagat gatggagggt catgttctta tcatattaaa gatgttcata
    11041 aatactcttt atcagaaaat gaattttcaa aagatagaga tgaagaattt aaggatagct
    11101 gtgaagcaat gggtgtaaaa gaaagtaata tacatataga agataataga gcacatgatg
    11161 gtgagttaag taaagaaaag gcaagggaaa taatacttaa gtacttagaa gaatatccag
    11221 atgcaaaagt aaaaactgtt actccgttta aagctagtgg aatacatgaa gaccataggg
    11281 cattaggaga agctgcacta gagttatata aagaaggcaa aataaaagat ttaagatttt
    11341 atgtagaacc atatgactat aaggatttta aaaaagttaa tccaaatgta gaggtatgga
    11401 aggtattacc tagtcaagaa gagaaattat taagtgcaat gaatgcatat aaaaaatgga
    11461 atcctgaaag tggacattat gctataggat accattcagt aaaatctcat tttgatgagc
    11521 tagctacaaa taaaatacag tatgtacatg ctccataaaa tattatgaaa gaattaggag
    11581 gtaagaatta atgaaaatat caaaaaagat agtgtccttg ttaactataa catttttaac
    11641 tattatgtta tatggaaata catctaatgc atctacaaaa gatacattaa caggttctgg
    11701 aagatgggaa acagcaataa aaataagtca agctggatgg acaaagtctg aaagtgctgt
    11761 attagtaaat gataattcaa tagcagatgc tttatcagct actccatttg caaaagcaaa
    11821 aaatgcgccg atattattaa ctcaaagtaa taaattagat gatagaacga aagtagaatt
    11881 aaaaaggtta ggtgtaaaaa atgtatattt gataggagga acaaatacat tagatattaa
    11941 tgtagaaaag caattaaaag cggaaaatat aagttttgag agaatatcag gaaataatcg
    12001 atatcaaact tctttaaagt tagctgagaa attaagtaaa gaaaaggaaa tttctaatat
    12061 agttgtagtt aatggagaaa aaggtttagc agatgcagta agtgtaggtt ctgtagctgc
    12121 gcaagaaaat atgccgatta tactttctga tacaataaat gggataaaag atacagaaaa
    12181 atttataaaa gaaaaaaata taaaaaaatc atatatagta ggggggatat attctgtttc
    12241 taattctgta gaacaaagtc tccctaattc taaaagaata gctggaaata atagaaacga
    12301 aacaaatgca aaagttatag aagagtttta taaaaataca gatttgaaaa atgtatatat
    12361 aactaaagat ggtatgaaaa agcaaagtga cttaatagac tctttggcag taggagtttt
    12421 ggcagctaag aataattcgc cagttgtttt agtaggaaac aaacttgact ttactcagaa
    12481 tgatgtatta aatactaaaa ttatcaaaaa tgttgttcaa gttggtggat taggaaatga
    12541 aaatgctgtg gaaagtataa ttgattcgca agaagaaacc acatacacag ttaaaaatac
    12601 tgatgaatta aatgtagcca tcaaaaaatc agatgctaat gatattataa agtttaagcc
    12661 tgaaaaagaa aaaaatatta ctgaatcttt taaagtagaa acagacaaat ctataagtgt
    12721 agagtttgaa gggacatata ctcaaactat aagtataaat atgccaaatg gtagcataac
    12781 taatcttgga gatgtaaatg gtaccataaa aataatggat gtaagatcta gtacattagt
    12841 taataagggt gaaataaatt atcttgatat ttatgataat aatggatgca gagttgaaaa
    12901 tgaaagagat ggagatatat ggttagtaac tatatctaat gaatccagag atgtgcatat
    12961 agaaaatgat ggagatataa ataaaatatc taatagttgt tctagtgtaa caataaaaaa
    13021 ttcaggaaat attgatactt tgagtggaag taaagagata gcgataagtg gaaaaaaacc
    13081 aaaagttgat gatacagata aagatgatga aagagctagt ggtataaaca ttaaagttga
    13141 gccttgttct atacctaaga gagattatgt aaaagttaga atagtcactg agcctaaaaa
    13201 ttcaaattat aaaatctact ataaagtagt gtccaaaaaa cccacatcta gatatgttgg
    13261 agataagata aaagtaagtg aatgggatga tgcaccttct gcaggaacat catttgaagt
    13321 tgaagcaaga aatgggtttt atatagaagc tgttgaaata aatacttcaa caaataaaat
    13381 ttctaaatgg ggtaggtcag attcaacaga tgatggtttt aaatcaatag aggtggcaaa
    13441 aggtttaagt gcaaatgtat ttatagatgg tgataaggtt gaactaacga caacaagagc
    13501 aaattctgga tgtgaaattt actatagaat tatgtctagt aaaccaacag caatgaatgt
    13561 tggaagtagt attactttat ctagttggga aaaaattagt tccagtagtt tagaattaga
    13621 gtcttctgaa gtaagtgaaa aatatattga attagtagaa ctagaaaaat ctactaattt
    13681 agtaactaga tggggttcaa ctgaaaaaat aaatatacca aattctagta tataaaaaaa
    13741 taaagtagtc taacatattt gtaggctact ttatttttct ttaaaaatta tcattttact
    13801 aaacaaataa tttaaaacta taacaactat attagtaaat acttttacaa ttatatcatt
    13861 tacaaaaagc aaatcaatca ttacatacat aattaacatt tcaacgacac cagaaagtag
    13921 cctaaaacta acaaaagaca aaaattcctt aaataagaac tctatttcaa ttcttttact
    13981 ctcaaacaca aaaaacttat tagtaacata agcaaataaa acagcaagta tccatgccaa
    14041 agcatttgca accataaaat taaataaaat aactctagta aaaaataaat acgaaactat
    14101 atttacaaga gtagtaaatg ctccaaaaaa taaatataat attgtctctt tatgtttttt
    14161 aaaaatcaaa tataaccctc ctaaactaat caaatataac tagctttaaa taaaattaac
    14221 acattttagt ataacacatc tattaataaa tcaatatatc ttaaaatatt ataaaaataa
    14281 aactataata ttctataatt aatatatact cataatccat attttatcaa aataaaatat
    14341 gaccttaatt tagaaatctg atataataaa aattaaatac atataagggg gtaaacatga
    14401 gaaagtacaa atcaaaaaaa ttgtcaaagc tactagcatt gttgacagtt tgctttttaa
    14461 tagtgtcaac aatacctgtc tcagcagaaa accataaaac tctagatgga gtagaaactg
    14521 cagagtattc tgaaagctat cttcaatacc tagaagatgt caaaaatgga gacacagcaa
    14581 aatataacgg agtaatacct tttccacatg aaatggaagg tacaacactt cgaaataagg
    14641 gcagaagtag tcttccatca gcatataaat caagtgtagc atacaaccca atggatcttg
    14701 gtcttactac accagcaaaa aatcaaggaa gtcttaatac atgctggtct ttttcaggta
    14761 tgtcaacttt agaagcatat cttaaattaa aaggatatgg aacgtatgac ttatcagaag
    14821 aacatttaag atggtgggca actggtggaa aatatggatg gaacttagat gatatgtcag
    14881 ggagttcaaa tgtaacagct ataggatatc tgacagcatg ggcaggtcct aagttagaaa
    14941 aggacatacc atataatctt aaatctgagg cacaaggtgc aactaaacct tcaaatatgg
    15001 atactgcacc tactcaattt aatgtaacag atgttgttcg tcttaataaa gataaggaaa
    15061 ctgtaaagaa tgctataatg caatatggtt cagtgacatc aggttatgca cattattcaa
    15121 catattttaa taaagatgaa acagcatata actgtacaaa taagagagct ccattaaatc
    15181 acgctgtagc gatagtagga tgggatgata attattcaaa agataacttt gcatctgatg
    15241 taaaaccaga atcaaatgga gcatggttag taaaaagtag ttggggagag tttaattcta
    15301 tgaaaggatt cttctggatt tcttatgaag ataaaactct actaacagat acagataact
    15361 acgcaatgaa atcagtatca aaaccagata gtgataaaaa aatgtaccaa cttgaatatg
    15421 ctggtcttag caagataatg tcaaataaag taacagcagc aaatgtattt gattttagca
    15481 gagactctga aaaacttgat tctgttatgt ttgaaacaga ctctgtagga gcaaaatatg
    15541 aagtatatta tgcaccagta gtaaatggag ttcctcaaaa caattcaatg acaaaacttg
    15601 caagtggaac agtatcatat tctggataca taaatgtacc tactaattct tacagcttac
    15661 caaaaggtaa aggagcaata gtagtagtta tagacaacac agcaaatcct aatagagaaa
    15721 aatcaacttt agcatatgaa actaatatag atgcatatta tttatatgag gctaaagcaa
    15781 acttaggtga aagctacata cttcaaaaca ataagtttga agatataaat acatatagtg
    15841 aattttctcc ttgtaacttt gttataaaag ctataacaaa aacatcttct ggacaagcta
    15901 cttcaggaga atctttaact ggagcagata gatatgaaac agcagttaaa gttagtcaaa
    15961 aaggatggac ttcttcacaa aatgcagtat tagtaaatgg agacgcaata gtggatgctt
    16021 taacagctac accatttaca gcagcaatcg actctccaat tcttttgaca ggaaaagata
    16081 acttagattc aaaaactaag gcagagttac aaagattagg aactaaaaaa gtttatctaa
    16141 taggtggaga aaattcttta agcaagaatg tacaaactca acttagtaat atggatatat
    16201 cagtagaaag aatttcaggt agtgatagat acaagaccag tatatctcta gctcaaaagt
    16261 taaatagtat aaaatctgtt tcacaagttg cagtggcaaa tggtgtaaat ggacttgcag
    16321 atgcaataag tgttggtgca gcagctgctg ataacaatat gccaataata cttactaatg
    16381 aaaaaagtga gttgcaaggt gctgatgagt ttttaaattc atcaaaaata actaagtctt
    16441 atataattgg tggaacagct actttatcat caaatttaga aagtaagctt tcaaatccaa
    16501 caagacttgc aggaagtaat agaaatgaaa ctaatgctaa gataatagat aaattctatc
    16561 ctagctcaga tttgaaatat gcttttgttg ttaaagatgg ttcaaagagt caaggagact
    16621 tgatagatgg actagcagta ggagcattag gtgctaaaac tgattcacca gtagttttag
    16681 ttggaaataa gttagatgaa agtcaaaaaa atgtacttaa gtctaagaaa atagaaactc
    16741 ctattagagt tggtggaaat ggaaatgaaa atgcttttaa tgaactaaat actcttttag
    16801 gaaaatagtg aatggaaaga tagtaaggat atacatttta ttattaaaaa atgttcaata
    16861 gaagtttata aatataaaat aacaaaaagg ggtgtctcaa aatgaacttt ttagttcatg
    16921 aggcactctt ttttgttgtt ttatattttt atcatttaaa taatgaaaaa taggattgtc
    16981 tatattttta gatagttttc ttattattga tgaaaaatgt aataatttta aactgtaaaa
    17041 tagtgtaaag ttattttata atataaatat agtaagattt taaacaaaat aagggggaat
    17101 aataatgaaa gcaccaaaaa ctattttaac aatactaaca atagcgctta ctttaagtag
    17161 catttctata ataccatcat atgcacttac agaggaaaaa ttaataggta atggtagata
    17221 tgaaactgct gtaaagataa gtcaaaaggc atacagttct agttctaatg tagtgttagt
    17281 taatgacaat tcattagctg atgcactttc agctacacca tttgcaaagg ctaaaggagc
    17341 accaatactt ttgacagaaa gtgataagct agatgatagg actgaaaaag agataaaacg
    17401 tctaggagct aaagatattt atttgatagg aggtactgct gtacttaata aagatataga
    17461 gaacaagtta aaaggtaatg gacttaatgt agaaagaatt aatggtaagg atagatatga
    17521 aacttcactg attttggcta ataagcttaa ggatataaaa gatattaagg aagttgctgt
    17581 agtaaatggt gaaaaaggat tgagtgatgc tgttagtgtt ggagcaccag cagctcaaaa
    17641 taagatgcct ataattttgt ctagccctaa ggacggagta gaagcatttg ataaatttat
    17701 aagggatgag aaagttataa aagcatatgt aattggaggt acaaattctg tttcaagagc
    17761 tgtagaaaag aaccttccta atgcagaaag agtgagtggg aaagatagaa atgagactaa
    17821 tgctaaggtg atagaaaagt tttatactga tacaaattta agtaatttat atgtaactaa
    17881 ggatggtagt aaaaatgaga atcaactaat agactcgtta gctgttggag tattggctgc
    17941 aaaaaatgag tctcctgttg ttttggttgg taataaatta aacacaaaac aaagagatat
    18001 acttagtact aaaaaattaa atactataac acaggtaggt ggaaatggaa atgaagaagc
    18061 ttttgatgaa attaaaagtc tacaagaaaa aactgtgttt gaagcaaaaa ctgttgagga
    18121 attaactgat atgataaata tagcaagtcc taatgatatt ataaacttta aaccaaaaga
    18181 gaatactgta aatgaagctt ttagaatggt aacaaataaa ccaataactg tgaatataaa
    18241 gggtgattgt tcaaagactc ttacagtaga tatgcctaat ggtgaagtga ataactatgc
    18301 tactttggta aatgttatag ttagaaatat tggagaaggt ggatttaata atcatgatac
    18361 aatcactata ttgtcagttc gtgataaaaa tggtagagtt atagaaaata ctagaaattc
    18421 agatatagat acgttgatga tattagcttc tgctaatgat acaaaattga ttaatgatgg
    18481 atatatagga aaacttatag ataattcatc taatagtgat ataactaata atggtactat
    18541 agataaaaaa gttaatcagg ttgaagattt agaggctaaa gtagattcaa ttgaaaaagc
    18601 tatagattct ataagtcaaa aagtcaataa aatacaagat atactagata agcttggatt
    18661 tctaaagaaa tttcttagct aagaatatgt atatttaagt gaagtgtgtg ttgtaattgt
    18721 tctaaagtta tatagttaaa caggtttata tacaaataga tttatagata aatatgtagt
    18781 tcagtatata tttgaataag ctttagggag gtaacatggg gattggaatt gaatgtaaca
    18841 aaaaaataaa taaaagtatt gacaaaacta gaataaagaa aaataatct
//
LOCUS       DAEMHW010000065        18827 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEMHW010000065 DAEMHW010000000
VERSION     DAEMHW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.065"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            195..581
                     /locus_tag="KPK67_001890"
     CDS             195..581
                     /locus_tag="KPK67_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2458859.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            731..994
                     /locus_tag="KPK67_001891"
     CDS             731..994
                     /locus_tag="KPK67_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2458860.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(1123..1965)
                     /locus_tag="KPK67_001892"
     CDS             complement(1123..1965)
                     /locus_tag="KPK67_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2458861.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            2260..2994
                     /locus_tag="KPK67_001893"
     CDS             2260..2994
                     /locus_tag="KPK67_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2458862.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            3004..4137
                     /gene="nagA"
                     /locus_tag="KPK67_001894"
     CDS             3004..4137
                     /gene="nagA"
                     /locus_tag="KPK67_001894"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG2458863.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGEVVYKNI"
     gene            4236..4985
                     /gene="nagB"
                     /locus_tag="KPK67_001895"
     CDS             4236..4985
                     /gene="nagB"
                     /locus_tag="KPK67_001895"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBG2458864.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            5137..5409
                     /locus_tag="KPK67_001896"
     CDS             5137..5409
                     /locus_tag="KPK67_001896"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458865.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            6069..6773
                     /locus_tag="KPK67_001897"
     CDS             6069..6773
                     /locus_tag="KPK67_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2458866.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            6775..8262
                     /locus_tag="KPK67_001898"
     CDS             6775..8262
                     /locus_tag="KPK67_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2458867.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDANTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHARDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            8467..10080
                     /locus_tag="KPK67_001899"
     CDS             8467..10080
                     /locus_tag="KPK67_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2458868.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(10443..11273)
                     /locus_tag="KPK67_001900"
     CDS             complement(10443..11273)
                     /locus_tag="KPK67_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2458869.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGFYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            11346..13073
                     /locus_tag="KPK67_001901"
     CDS             11346..13073
                     /locus_tag="KPK67_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2458870.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            13110..14972
                     /locus_tag="KPK67_001902"
     CDS             13110..14972
                     /locus_tag="KPK67_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2458871.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            15305..15994
                     /locus_tag="KPK67_001903"
     CDS             15305..15994
                     /locus_tag="KPK67_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBG2458872.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDDNMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            16099..16785
                     /locus_tag="KPK67_001904"
     CDS             16099..16785
                     /locus_tag="KPK67_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBG2458873.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            16802..18709
                     /locus_tag="KPK67_001905"
     CDS             16802..18709
                     /locus_tag="KPK67_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBG2458874.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSADLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISLLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
BASE COUNT         7329 a         2025 c         3206 g         6267 t
ORIGIN      
        1 attaaaggtg ttagaaaatg tagtaaattt tatactatca gactctatct ttattacttt
       61 agtagataca tggttgtaaa ggtttataga aaaaaatata ggaaggcaga tttttattgt
      121 tgcgtatata tgagaatgat ataatatgaa tattataaat taataaattt aaatttatgg
      181 aggtacaaga gatgatggaa tataaagtta atgaccaaga acttaatgct tcaatgttta
      241 tcccattcgt caacaggata tggccaggag attatgatat ggataaaaca cagactgcat
      301 tatcaaaaac tttgaatata actgcttatg atgataaagt gcttgtagga tgtctgcgag
      361 ttctttcaga tggttattat tttggaacaa ttacagaatt gcttgttctt ccagagtatc
      421 agaaacaagg cattggtagt aagcttttcc agcttgctaa agagaacacc ccaactatgc
      481 tatattttgg tgcacagcca ggagtagaag agttctatga aaaaaatggg tgccaaagaa
      541 gtctgcaatc ctacataata aaaaagaaag atagttatta aagttccagc tttcaagaat
      601 cttaaaagta caatatttta gtggaatcat ttcatttttt attactaata tgacataaac
      661 atgtcatatt agtattgata tacttttaat atggttaaga gccaaagaaa aatataagga
      721 gtgatttaaa atggaagaaa ctaaattttg ccaatgctgt gcaatgccaa tgggacaaac
      781 tgatgaatta tatgggacaa ataaagatgg aagtaaaagt actgattatt gtagttattg
      841 ttttaaagat ggtgagttta cagctgatat aagtatggaa gagatgatag aagtttgtat
      901 ccctcatatg cttcaaagta ataaggatat gactgaagat gaagcaagaa caatgatgaa
      961 caatttcttt ccaacattaa aacgctggaa ataattttat gcaaaattag ttatatgcaa
     1021 aacatgaaat atggtgttga gaataagggg tggtgtgctt tatagcatac cacctgtttt
     1081 gttacttgga gttcagttat tttcctaaat attttaaata tactatttta gataagttcc
     1141 ctcttcatct tgataaatta atcctttttt cataagcact ccaagagctc gtttaaaatt
     1201 tttcttactt gaattaaata atatagaaat atctttaggg tcagatttat cattaaatct
     1261 catatatcca tcagaacctt ctaaataact taaaatctta ttttctaaag tatcaagttc
     1321 atcctttcta tttcctctag gagaaagacc aagttttcca tcttcatata tttttatgac
     1381 atgtaaattt tcaagaacat caccagtttt tagttcagta aagtactcat tatgaagtat
     1441 tacaccagca tatttattgt ctacacatat cattgcagaa ttattagttt ggaatccata
     1501 tacaactcca gttacaatat ctcctacatt gtaagtatgg tctgttaata agtatgaatc
     1561 tatatctgta gtagcagcaa ttcttttgct tttgtctaaa tatatataaa ataaatattt
     1621 ttcgtctcta aatagtggat aagtcttagc tttaaaagga actaatatgt ctttaggaag
     1681 tcccatatct ataaaagtac caatgtctgt attatcaact acttttaaat gtgctacatc
     1741 cccaacctta gctaaaggcg gtattaaagt tgcagcagtt ctgtcatcag aatctttaaa
     1801 tatgaaagca tttacttcat cacctatgtt tatttcattt tttccgatta atctattatg
     1861 aagaagtata tcgtcttttg tgttattagt ttgtccatcc aaaaaatatc caaattcagt
     1921 ttttcttttt actgtaagtt tattaaaatc acctatgttt atcaaattaa aatcatcctc
     1981 tcattttata tttataattt agccataata tacttatgta gtagtttgtc acagtatcta
     2041 tattagtatt agtgaaattt acaataaata atatagttat ttatgtattt ttcaattctc
     2101 tagcttctta tatagtataa actccaattt cttttttgtc tttaggtttt attaaaattt
     2161 ttacaaaata agttaaaaag tattgaaata aaatttcttt taaagtataa ttggtatata
     2221 ccaaaattgg tatataccat atcgaaaagt ggtgactata tgaaaatagt aaataaatct
     2281 agtaatatac cattgcatac tcaattaagt tcaataataa gagagatgat agaaactggt
     2341 gagcttaaag agggtgatgc aataatgcca gaaagagaac tttgcaacat acaaaatgta
     2401 agtcgtatga cagtaaataa aaccattgtt ggattagtta cagaaggact tctttatagg
     2461 gtacaaggta aagggacatt cgttgcaaag caaaaaaaga agtatcagtt ttcaaatgta
     2521 aaaggtttta ctgatgttat gaaagaaaag ggagtaaaca taaaaacgga tatattatcc
     2581 tttgagatgg aactaccaga tgatttagtt aaaagaaagc ttggaataag tgataatact
     2641 acaaatatat ataagattgt cagacttaga tatactgatg gagagccatt tggattagaa
     2701 atagtatatc tatcagaaga aatgtgtaag ggtctaacaa aagggatatt ggataattct
     2761 tcattatata gggttttaaa tgaaaaatat ggctataaga tacaaaaagc tgaacaggta
     2821 atggagccag taatactttc agatgaagaa agtaagcttc ttgaaacaga tgaaggtgct
     2881 ttagctctta aacttcatag aaattcctat aatagagaag gtagccctat agaatatacc
     2941 atatcaatat ttagaacaga taaatatcaa tatgaaatag tattgagtga gtaggtgaat
     3001 aatatgaaat gtttaattaa tggaaagata atattaaaaa atcaaatctt agaaaataaa
     3061 gtattagtat ttgatgaaaa aataattgat atagctgata gtgtaccaaa agattgtgaa
     3121 gttatagatg ctgatggaaa atatatatct ccagggctta ttgatattca tatacatgga
     3181 aacatgggaa aagatacaat ggattcgact gatgaatcta ttgaaacaat ttcaaaatca
     3241 attatgagac atggagtaac atcttttctg ccaactacaa tgactatgga taaagaacat
     3301 gtttatgatg cacttgaagt tattaaaaaa gcccaaaata gaaagcttga aggggcacaa
     3361 gtattaggag cacatttaga aggacctttt ataaatgaga attataaggg agcacaaaat
     3421 gaaaaattta taataaattc aaaatacgaa tttataaaag aatataaaga tgttataaaa
     3481 gttataactt atgctccaga aaaagatatt gattttgatt ttactagaga aataaaaaga
     3541 tgtacagata tagttttatc tattggtcat agtaatgcaa attatgacca agctaaagaa
     3601 gctataaatt taggagtaac taatgtaact cacatgttca atgcaatgac tggtttaaac
     3661 catagagacc caggcgttgt tggtgcagca cttacaacta atgtgtacag tgagttaata
     3721 gcagatacaa tccatataaa taaagattta tttcaattta tactaaataa taaaggaaaa
     3781 gaaagactga ttttaataac agattctata gaagctggtg gtcttgaaga tggcaattat
     3841 tctcttggag gtcagaaagt catagttaag ggtaatgaag ctagacttga aaatggagca
     3901 ttagctggaa gtgtgttgtc tttaaataaa atggtattta acttcttaga taatacaaac
     3961 cttaaagtga atgaagcaat taatttagct tctttaaatc cagcaacatc tttgggaata
     4021 aatgacaaaa aaggaagctt agaaataggt aaggatgcag atatagctgt atttgatgag
     4081 aatttagatt gtaaaatgac actttgcttg ggggaagtag tatataaaaa tatatagtat
     4141 acaaaacaaa ccttaaagac agtaaaaaca atataaaaaa taataaatta aacttacatt
     4201 agtaaaagta gtagttaaaa tttaggagga ataagatgag agtattagtg tgtaaagatt
     4261 atgatggaat gagtaaaaaa gccgctgaaa tgatagcagc tcaaatagtt ttaaaaccaa
     4321 attcaatact gggactagct acaggaagta caccagtagg tatgtataga gatttagtaa
     4381 aaaaatacaa tgataatata gtagattttt cagatgtgat gagctttaat ttagatgaat
     4441 attataagtt accaatatct aatgaccaaa gctatgatta ttttatgaaa gagaatctat
     4501 ttaatcatgt aaacataaag ccagaaaata ctcatctacc aaatggaatg gctgatgata
     4561 ttgaaaaaga gtgtatgaac tatgaagctt ctatagatgc agctggtggt atagatgtac
     4621 aagtacttgg aataggtcgt aatgctcata taggttttaa tgaacctgat actaaatttg
     4681 caaaaagaac tcatgtagtt gaacttacag agtctacaat agaagctaat gcaagattct
     4741 ttaaatcaag agaagatgtt cctaaaaaag ctgtaagtat gggaataggt tcaatattga
     4801 agtctaagaa aatattgctt ttagcttcag gagaagaaaa agctgatgca gtatataata
     4861 ctgtttacgg agatataact ccagaagttc ctggttctat acttcaactt cataaagaca
     4921 ctatagttat agtagatgaa gcagcagcaa gtaaactgaa tccaaaagat tataaattag
     4981 tataagaata gaatgtagtt taagtaaaaa atagctatca ttgaataatc aaatttattt
     5041 aatgatagct atttttattt aaattagtta ttattgttta aattacttgc ttattacttt
     5101 ttacgaaaac tcgttatcaa gaagtatcca aataagatgt taaaaattat tatgtttagt
     5161 gggattctat caagcataat aaaattacct ataattggga tattaccaac tggaaacata
     5221 aagaagaata caggcatcag tgatagtaaa aaagatggaa aacaaagaag aagtaatctt
     5281 ggaatatcaa tataccaact accatccttt ttgtattctc taatcaataa ttcagttcct
     5341 agtaataaac caataccacc agaggaaatt aaattaataa aaatgtatct tttaaagtct
     5401 tgcatataac cagtgtaaaa ttgagtatag aaatcataat ttaatttaac ggctaaaaaa
     5461 tacataagca atagaatgat taaaaataaa agatatctaa gagctttttt cattttaaat
     5521 tctcccttca gaaatacttt atttagtttt attttgttgg aatctgattt tatcatttag
     5581 tttaagaaat ccataatatt agtgaagaat gaaataagta ttatatatga ataaatattt
     5641 ttagaattaa ggtgaattgt agtgcttaat ataactataa tattaattgt ttgaatattc
     5701 aataaaaagt ttgaaaaata tcaattctga tataaactga ataaatacag ttgaatatta
     5761 aattattatt ttatcaaaaa tatgcaaagt actacacctt caaatatcac ctaaagtaat
     5821 taaaaggcaa ttaaagtgaa gtagttcaag tatttctaaa ataagtttaa taaattctat
     5881 aataatttat tttttgcctt aaaattgcct taaaatccaa acaattttaa aactaatgta
     5941 ctataaaaag ctggtataat ttaaatatag taaaaaataa gatttatctt atctattggg
     6001 atttaattat ttaatcaata tatttatacc atttaattta aataaaaagt ctattaagga
     6061 gtgagcttat gagtaagtta atttatatag cagatgatga agataatata agaaatttgg
     6121 taaagacatt cttaaaaaat gaaggtcatg atgttatgga ttttaaaacg ggtgatgaac
     6181 ttttagaaca atttaatata aaggagtgtg acttagtaat actagatata atgatgcctg
     6241 gctcaagtgg ttttgaagta tgtacaaaac ttcgtgagaa aagtacagtc ccaataatca
     6301 tgcttactgc aagagataca gatatagatt atattacagg gataacctta ggtagtgatg
     6361 attattttac aaaaccattt agcccaatgt ctttggttat gagagtaaaa tctatattta
     6421 gaaggataga gtttgaaaag aaacaaaatt atgataaata tagtaactcg atagatatgg
     6481 aattaaaatt tggtgatgta ataataaata aaaagaataa aatcgttaca agtaaaaatg
     6541 taaatataga ccttacacca aatgaatata acctattgac ttatttgttt gaaaatatag
     6601 atagagcagt ttccagagat gaattattaa acaaaatatg gggatacgat atagaagttg
     6661 agacaagggc tgctgatgat acagtgaaga gattacgtaa aaaaatttta gatacaaata
     6721 ttttaataga aactgtatgg gggtttggat ttaggttgaa ggagaaaagt taagatgaag
     6781 aatggaaatg acaagaatag aattggaaag attaaatcca aatttaaatt aaagttctca
     6841 aagaaaaata taagcataaa aactaggatt ttgttgagta tactttgttt gataattata
     6901 gtatttatga taatattagt atcttttaat gtatttcttg atacatatat aaaaacaact
     6961 gcgaatgaag aattgacaaa gtctacagaa atagtagaac atatggatag taattttaga
     7021 cctattaagc caccacaaga tgatggaaag cttcctccca aagtattatc taattttatg
     7081 agaaatgtac aagataaggt taggatggca gaaacacaat cagatgctga tgctatggta
     7141 gttgattcta agtataacct gatatttcca acaagagagg atgatttctt aaaaaatgtt
     7201 gatgagatgg agctgattag ggagtgtatt caaaatgaaa aattagattt aaattcagat
     7261 gcaaatacac ggatatctac cagaaataag gattattata tttcaactgt aaaaattacc
     7321 agtatatatg gtgaacaaga caagtatctt atacttttta tagacatatc aaaaactcta
     7381 aatctagcac aaaagataaa tattgtatta atatctgtaa tgtgttttgc tggaatatta
     7441 gcaatattta cggctgtgat tttatctgag aaaattgcta aaccgataaa agagttgtgt
     7501 gagtttgcaa aaaaaatggg tcaaggagat ttcaaaagaa cctactttga cttttcagat
     7561 aaagaattag tagagctctc aactgttatg aataagagtg ctgagtatct ggataagtat
     7621 gataatgaac aaaaaatatt ttttcaaaat gcatctcatg aattaaggac accactcatg
     7681 tctattaaag gttatgcaga agctataaaa tataatgtta tagatagtaa acatgcaaga
     7741 gatataattc ttgaagaaag tgataggtta agtgatatgg ttgaagattt attatacatt
     7801 tctaaaatag ataatataac taaagatttt gaacttgtag aatgtgattt aagagaagta
     7861 ctttcaaatt gtggagctag gcaaaatgta agagctataa ataaggggat aaaatttatt
     7921 tatgaatttg atgaagaaat ggttttattt gaatgtgatg aaaaaaacat ttccaaagca
     7981 tttatgaatc ttatagaaaa tgctttgaga tacgctaaga gtgaaataaa aatagtgtgt
     8041 aagtataatc aaaaaaatat agttgtgata atagaagatg atggaattgg aatcaagaaa
     8101 gaagatttac ctcatgtttt tgaaagattc tataagggag taggaggaaa tcatggaatt
     8161 ggattatcaa tagttaaatc tattgtaaat aagcatggtg ggcgtatata cgtagaaaat
     8221 ggcaaaaaag gagctaaatt tactatatta tttaaattgt aaaaaaatta gagaaaatat
     8281 agagatgttt gaagttgttt tttatatata tctgttgtat actgttgaaa aaagaacaat
     8341 attaagctga catatattac gatagttgac atttaaagta aatgcttaaa tatagatacg
     8401 aaaggaaaag taaaatctga agtgtattta ggtttaagta agcttcttat tttataaaaa
     8461 atatgtatga aagaacagct gatttttaat attacaaatt tagtaacggt tgtatttgaa
     8521 gcttttgtca tccatatgtt tttatcagac tttttaggcc agaaagaagg gtatataaaa
     8581 gttgtaagat atgctaaact gggttttata atatgtttag gattctgtaa tttaataact
     8641 ttaaacccta aaatcacaat gcctttaatt ttcatcctta ttttttcaac atcattttta
     8701 tataaaggaa atttaaagac acgattgttt actactgttt tattaagtat attctttata
     8761 ctgtcagaaa ttgtagttac aagtattttt gttttatttg taaaagaagg ctttgaaata
     8821 atgcttgaaa ataatagtat aagagtattg gcgacaattt tatctaaaat tgtctttttg
     8881 ttgacttgta aaattatttg tctatttaaa aaagatgtac atttagatat gcccataaag
     8941 tattggctcc ctttattttt aatacctata tttagtttat ttctgtctgt ttctatcttt
     9001 gatgtaagta aattttttag tcttgaaagt ttaaaatttc taagcttaat atctagtgtt
     9061 ggtattttat atattaattt tattgtcttt tatttattta aatttattat agataaaact
     9121 aaactatcta tgaaatatga actacttgaa aaagaaataa ttcataagga agaattaaga
     9181 cttagtaatg agtgttataa aattattgtt gagcagactg attctgtcgt tttcgaatgg
     9241 aatatcaaag aaaataaatc atttgtatcg caggcttgga ctgaaaaatt tggatataac
     9301 aatgcatgta agaatatttt taaagaaatt aaagataaag acttagttca ttctgaagat
     9361 aaagctatat ttgaaggttt tcttgaaagt attaaaaaaa agaatatgca caatcaagca
     9421 gtatatcgtt taaaaaaatc taatggtgaa tatatttggt gtagaacatc aattacaagc
     9481 atttataatg atgaaaatga acttttaaga gttgtcggag taattgtaga tgtagatagt
     9541 gatataaaaa aatatgaaga gttaagaact agagcagaaa gtgactcatt gaccaatata
     9601 tacaataaag gtacatttga aaaattagtg gaagaaacta tagttatgaa tacaggtgat
     9661 aaaaaagatg cattattcat tattgatttg gatgatttta aagaaataaa tgataatttt
     9721 ggtcatccat ttggtgattt tgtactaaaa acatttgctg ataagataca aactagtttt
     9781 ggtagtaaag acttagtcgg aagaattggt ggagatgaat ttgttgtata tatgcaagat
     9841 tatgttacag aagtaaattt gcataaaaaa gctaaagaat taaatagagt actttcggat
     9901 aattacactg atttgagttt tagttttgat gcttctgtca gtataggtat tgcaaggtat
     9961 cctcaagatg gaacttcatt ttttgaattg tttaaaaatg cagatagagc actttatagc
    10021 ataaaagcat caggtaaaaa ttcatattgt ttatttgaag aagaactata tgtacagtga
    10081 atttgaattt agattttatg tgagagtaag atttatatat acttgtggct gtctgattta
    10141 taaaatatgt tactttgtat atttttatat gtttcagtaa ggttaattaa cataagcaaa
    10201 ggtgtaagta aatttatatt agtttaagtt tacttacacc tttataaaaa tacaaataat
    10261 agattcattt acattcttaa atgattaaag ctctttacct tctaaacatg cataacatat
    10321 cgttataatt ccagtaaata agactaaaat gcccattcct aagattacaa tttcatcatc
    10381 tattactcca aatgttttta aagtaactta tttcgctttg tatcataata atttgtctcc
    10441 ttttacattt taacttctgc tgagatttga gttagatatt cgttagaatc tcgcacactt
    10501 atatcatata gaagatattc ttcataaaca aaatccccaa tacgtaaatt attctgatta
    10561 aaaaaattta ataacttttc ataagccttg taaatagttt cataactccc tttatgataa
    10621 gcagttacat aaaatccctt tggtttgatt gatacaggat atttcttgta ctctttatta
    10681 acttttgtaa ataaatatga atatgattca aagtctttat taattatatc ttcaccttta
    10741 atgattgagc caatcgtacc ataatcactc aattcatagt tgtccataaa gttaatacat
    10801 tcatataaaa aattttcttc ctcatctcct aaaaactgtt ctttatatgt aacaggtgtt
    10861 ttgaccaagt actcttcttc atgttctttt aaagtaatag tattcgcatc tatattacat
    10921 gcacttttag taaatgaaat agtagattcc attagagctt gaatattatt tagtttttca
    10981 atttcatttt taatatcaat ggttttcttt tctaaaagct caattagtaa attaggtgtt
    11041 cttttgtcta tataagcttt aatttctttt aaaggcattt ttacttccct aagtgaagaa
    11101 attataccaa aaatgctaag ttgatggtat gagtagtatc tatatccatt ttctttcttt
    11161 aattctggtg agaaaagacc tatatcatca taatggaaaa gtgttttttt attgattcca
    11221 caaatctttg caaattctcc agttgtaaaa taattttttg aaacttgact cataattata
    11281 attcctcctt gactatcctg ttacagtata gtttatcata gtattttgta aaggagtgtg
    11341 attatatgaa aaaattaatt cattttttaa aaccatatag agttttaatt gttgtagttt
    11401 tgatatttac gtttttacaa acacttggaa ctttgtatat accaacactt acagcaaata
    11461 tagtaaataa tggtgtagta aagggagata tagattatat tgtaaaaaca ggtttaatga
    11521 tgatgatagt agctggtata acagctttat cagctgtgtt agtttgtaaa gtatcagcaa
    11581 atctttcatc tggattttgt agagatatta gagaagcagt atttataaaa tcacaagatt
    11641 tatcaataaa tgattttaat aatataggta cagcatcaat gattactagg agtactagtg
    11701 atattacttt aataggtcag tctgtgttta tgtttattca attggtatta ccagcaccaa
    11761 ttataactgt ttctggtcta ttcttagcat attctataga taaagcaatg acaattatta
    11821 ttgttgtagt aatgttttta ttcatgcttt cagcattttt agttggtaaa aagcttataa
    11881 agttgtttaa aatgatgcaa ataaagatgg acaatatgaa tagggtgtta cgtgaggtag
    11941 taactggagt aagagttata agagctttta ataggagtcg ttttgaaaaa aagagatttg
    12001 atagaactgc aatagattat tctgaaacag caatttcaat aaataaaata tttgcagtac
    12061 ttatgcctat tgtaatgtta ataatgaatt taggtatagt aagtattatt tggtttggtg
    12121 ggatgagagt cagtaatgga aatatggaaa taggtcatat aatggcactt gttgaatatt
    12181 gtatcttaat tttattctat ttaatcatgg gagtaatggt atttatgtac atacctagag
    12241 caggtgcatg tgcagataga gtaaatcaga tattagatat agaacctgaa attgtagatg
    12301 gaaatggtca taaagatact gtctcagaga gaggacattt agtatttaaa aatgtaacct
    12361 tttcatatgc acaatcagaa gaacctgtgt taaataatat aacatttgaa gcaaaatctg
    12421 gagaagtaac tgcaattata ggaagcactg gctcaggcaa atctactatt gcaaatataa
    12481 taccacgttt ttttgaaatt caatctggag aaatatctat aaatggtcag gatattaaga
    12541 agattcctca aaaagaactt agagataaga ttggatttgt accacaaaaa gcttttttat
    12601 ttagtggaac aatagaagaa aatataaggt atggaaaaga agatgcaagt attgaagaag
    12661 taaagcatgc tgcaagtata gctcaagctg atgaatttat ttctgatatg gaagacaagt
    12721 atgactcatt tgtagcacaa ggtggaaata atttgtcagg tggtcaaaaa cagagaatat
    12781 ccattgcaag agcgttagtt agaaagcctg aagtatatgt ctttgatgat agtttttctg
    12841 cattagattt taaaacagat aaaagactta gaaaagcgtt gaaaaatgaa atcaaagatt
    12901 catcagctat aattatagca caacgtataa gtacaatcat ggatgctaat cagattatag
    12961 ttttaaatga tggaaaaatt gtaggtattg gaaaacataa agatttactt gaaaattgtg
    13021 aagtttataa gcaaattgcg gattcacaac ttagtaaaga agaacttgca taaaatgaag
    13081 aattaatctg atatttggag gtgtatgata tgagtgaaaa aagaatagat gaacaagata
    13141 tagaagtact tagttttgaa gatggcatgt cagcagaaaa agctaaggat gcaaaaaaaa
    13201 ctactaagag attattaaaa tatatggcaa aacaaaagtt taaaatatta attattttta
    13261 tttcagtatt gataagttca gcgcttacag ttttagcacc tatggttatg ggaaaagcta
    13321 ttgaccaact atttaatgga ataaaaactg cagtccaaac aggtacaaaa ttcagtgtga
    13381 attttagtac tatggggggg atagtatcaa tacttctagg gttgtactta attagtgcag
    13441 tatttatata tattcaacag tatattatgt caggagttgc acaaaatcta gttcttagca
    13501 tgagaaaaga tttaagtgat aaactcaata aacttcctct taagtactat gattctcata
    13561 aaaaggggga aacacttagt atagtaacaa atgatttaga aaaagtagca gatagtttac
    13621 aagaaggatt aatgcaactt attacagcag tagttacagt agttggttct attgtaatga
    13681 tgataagtat aagtgtacca cttacaatag tatcagctat aacattactt gtttcattgg
    13741 gtattactgt tgtaattgca agaaaatcac aagaaagatt ctctgaaaat caaaaggcac
    13801 taggagagtt aaatagcaat atagaagaga tatttactgg tcaaatagtg gttaaagcat
    13861 ttagtaaaga aaaggatact ataagaaact ttaaaatatt aaatcaaaat ctttataatg
    13921 caagtagaaa agcacagttt tcgtcatatg caatatctcc aattattaga tttataaacc
    13981 taattggcta tgttattata gcagttgtag gtgggatatt tgcatcaact ggagcaatga
    14041 ctcttggtag tatacaagcg tttattcaat atgtaaatca agcatcagag cctactacag
    14101 aaatatctta tattgtaaat atgcttcaag cagcaatagc atctgcagag agagttttta
    14161 cagttatgga cgaggtggaa gagataaagg atgaagaatc ttcaaaggtt attagcatgc
    14221 ctaaaggtaa agtacaattt gagcatgtta aatttggata tagtgatgat tttattctta
    14281 tgaaagatat taatatcaat ctaaatgcag gagataagat tgcaattgtt ggacctacag
    14341 gagctggaaa gacaactctt gtaaatttac ttatgagatt ttatgagata caaggtggaa
    14401 ggattactat tgatggtgtt aatataaaag atttaaagcg tggagaactt agaactatgt
    14461 ttggtatggt tcttcaagat acatggctgt ttaatggaag tataaaagaa aatattgcat
    14521 atagtaagtc agatgccaca atggatgaaa ttgtaagagc agcaaaatca gcaagagtag
    14581 accattttat aaggacttta ccacaagggt atgacactat attgactgag gatgcttcta
    14641 atctatctca aggtcaaaag caacttctaa caattgccag ggcaatactt tcagacccat
    14701 cagtattgat tcttgatgaa gcaacatcta gtgtagatac tagaacagag gtagaaattc
    14761 aaaaggcaat gaataacttg atgaagggaa gaactagttt tgttattgca catagattat
    14821 caactattcg agatgctgat ttaatattag taatgaaaga aggaactatt attgaacaag
    14881 gaagccataa ggaacttatt gaaaagaaag gattctatga agaactttat aacagtcaat
    14941 ttacaagtga atatgatgaa gatgttgtat gattatcaag ttttaggcaa ctaaatgatt
    15001 gataaatatt aagttaaata tagtaacatg taaggttata tggaataaga gtatttctct
    15061 atttcatata acctttttta tttatcaaaa taagataaaa tttcctttct aaattagatt
    15121 gaagaatgta tttatcaata taaaagtaaa ataatcatta ttattagtga agtaaatatt
    15181 gtttgccaca tatatgccat aaagatgcac ttattttacg gagatttaac aaagtagtgt
    15241 aaagtatact ctaaatataa aataaatcaa atagagttaa ataaattgag aggtgatata
    15301 aatagtgaat aaaaaattga actatagatt tgaaatgaag cacaaaataa ctgaagcaga
    15361 tgtgttagct ttaaaatcaa gactctatcc aataatgaag aaagatgaaa atgcatctaa
    15421 agatggaaaa tatcttatta ggagtttata ttttgatact cctgaagata aagcactttt
    15481 ggataaatta aatggcgttg caataagaga aaagtttagg ataagatttt ataataatga
    15541 ctgctcttat ataaggttag agaaaaaaat caaacattac aacatgacat caaagctatc
    15601 aactaattta accaaaaaag aagtaataaa tatactaaat aatgatattg aatttctaaa
    15661 agagtcgaca aattgtttat taagagaatt ttatttgaaa ttaaaatgtg aaagattaga
    15721 ggcaaaaagc atagttgatt acaatagaga agcttttatt tatccagttg gaaatgtaag
    15781 agttacaatc gatagtgata ttaaaacttc tgtaaattcg gtagatttat ttaataaaga
    15841 tttgccaact gtttcggtaa ttgatgataa tatgactgta ttggaggtta aatatgatga
    15901 atttatacca gattttataa aggatttaat tcaaattaat aagactacct ctacagcagt
    15961 ttcaaagtat gcttcaagta gattatatat ttaagaataa aggctatgat ttatataaag
    16021 atatacataa ataaggttaa aaaacaaata agttaaaaat aaataaaata aaaaataaat
    16081 aagttaaaaa taggaaggat gattttaatt atgacattta atgatatttt taaatcaagt
    16141 tttatagaaa acgtatcagg tttttcattt gtggacagtg ctttagcact aggttcagct
    16201 tttttagtag gtttgtttat atatatggtc tataaaaaga cttatatggg aattatgtat
    16261 tctcgaccat tcaatgtgtc gcttgtagca cttacaatgc ttacaacttt tgtaatattg
    16321 gcggttactt caaatgtagt tttatcctta ggtatggttg gtgctttatc tatagtaaga
    16381 tttagaacag caattaaaga cccaatggat ttagtatttc tattttggtc attgggttca
    16441 ggtatagttt taggagcagg tttgattccc ttagctgtca tgggttcaat aattatggga
    16501 ttgattctaa tattcttttc aaataaaaca atatctgaaa ctccatatat attgatggta
    16561 aattgtaata atgaagattc agaagatata gcaactgata aaattaaaaa agtatttact
    16621 aaatatcaaa taaaatcaaa aagtgttaca ccagaaaaag gaatagaatt agtgtttgaa
    16681 gtaagaatga aagatggaga gacaagtctt ataaatgact tgagtcaagt agatggagtt
    16741 actaatgcag ttcttgtaag ttataatggt gactatgtag catagagagg agtaatcttt
    16801 tatgaaagat aaaaaattta cccttcttat ctcgattatg attatatttt tatgtgctgt
    16861 agttggagtt tatagtacat ctagcaacaa aagtgctgat ttatatagtg atgtatatat
    16921 tgaaaaatat tttaacagag acaaggttat ggaagttaat atagagatag atgaaagtga
    16981 cttgaaggat atgaatgaaa atgctataaa agaagaattt aaggttgcaa aagtaactgt
    17041 agatggagat acatatggaa acgtaggtat aagaactaaa ggaaattcaa gtcttatatc
    17101 tgtagcaaat agtgatagtg atagatacag ctataagatt aattttgata agtataatac
    17161 tagtcaaagt atggaagggc ttactcaatt aaatcttaat aactgttact ctgacccatc
    17221 ttatatgaga gagtttttaa catatagtat ttgcgaggaa atgggattag cgactccaga
    17281 atttgcatat gctaaagtct ctataaatgg cgaatatcat ggtttgtatt tggcagtaga
    17341 aggattaaaa gagtcctatc ttgaaaataa ttttggtaat gtaactggag acttatataa
    17401 gtcagatgaa ggaagctcgt tgcaatataa aggagatgac ccagaaagtt actcaaactt
    17461 aatcgttgaa agtgataaaa agacagctga ttggtctaaa atcacaaaac tattaaaatc
    17521 tttggataca ggtgaagata ttgaaaaata tcttgatgta gattctgtcc ttaaaaatat
    17581 agcaataaat acagctttat taaaccttga tagctatcaa gggagttttg cccataacta
    17641 ttatttatat gagcaagatg gagtattttc tatgttacca tgggatttta atatgtcatt
    17701 tggtggattt agtggttttg gtggaggtag tcaatctata gcaattgatg aacctacgac
    17761 aggtaattta gaagacagac ctctcatatc cttgttatta aaaaatgaga catacaaaac
    17821 aaaataccat aaatatctgg aagagatagt aacaaaatac ctagattcag actatttaga
    17881 gaatatgaca acaaaattgc atgacatgat agcatcatat gtaaaagaag acccaacagc
    17941 attttatact tatgaagaat ttgaaaaaaa tataacatct tcaattgaag attctagtga
    18001 taataaggga tttggtaata aagggtttga caacaataac tctaataaca gtgattctaa
    18061 taataattct aatagtgaaa ataagcgctc tggaaatcaa agtgatgaaa aagaagttaa
    18121 tgctgaatta acatcaagcg tagtcaaagc taatacagat aatgaaacta aaaataaaac
    18181 tacaaatgat agtgaaagta agaataatac agataaagat aaaagtggaa atgataataa
    18241 tcaaaagcta gaaggtccta tgggtaaagg aggtaagtca ataccagggg ttttggaagt
    18301 tgcagaagat atgagtaaaa ctataaaatc tcaattaagt ggagaaactt cttcgacaaa
    18361 gcaaaactct ggtgatgaaa gttcaagtgg aattaaaggt agtgaaaagt ttgatgagga
    18421 tatgagtggt atgccagaac cacctgaggg aatggatggt aaaatgccac caggaatggg
    18481 taatatggat aagggagata tgaatggtaa aaatggcaat atgaatatgg atagaaatca
    18541 agataatcca agagaagctg gaggttttgg caatagagga ggaggctctg tgagtaaaac
    18601 aacaacatac ttcaaattaa ttttaggtgg agcttcaatg ataataatgt cgattatgtt
    18661 agttggtgta tcaagggtaa agagaagaag atttataaag tcaaaataag attaaaattg
    18721 aatataaata attttaatac tgttaagtct aaatatttac ttagcagtat tttttagaaa
    18781 ataatgtgtt tgattaataa tataaggtaa taaattgata aaaatta
//
LOCUS       DAEMHW010000066        18723 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEMHW010000066 DAEMHW010000000
VERSION     DAEMHW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.066"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..321
                     /locus_tag="KPK67_001906"
     CDS             <1..321
                     /locus_tag="KPK67_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG2458875.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKS
                     VYKK"
     gene            337..1920
                     /locus_tag="KPK67_001907"
     CDS             337..1920
                     /locus_tag="KPK67_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG2458876.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGRMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1948..3276
                     /gene="ssnA"
                     /locus_tag="KPK67_001908"
     CDS             1948..3276
                     /gene="ssnA"
                     /locus_tag="KPK67_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBG2458877.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3421..4773
                     /locus_tag="KPK67_001909"
     CDS             3421..4773
                     /locus_tag="KPK67_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG2458878.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            4965..6074
                     /locus_tag="KPK67_001910"
     CDS             4965..6074
                     /locus_tag="KPK67_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2458879.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6357..7748
                     /gene="hydA"
                     /locus_tag="KPK67_001911"
     CDS             6357..7748
                     /gene="hydA"
                     /locus_tag="KPK67_001911"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG2458880.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7742..10321
                     /gene="xdh"
                     /locus_tag="KPK67_001912"
     CDS             7742..10321
                     /gene="xdh"
                     /locus_tag="KPK67_001912"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBG2458881.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVNLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKSNKK
                     KKY"
     gene            10454..11800
                     /gene="rpoN"
                     /locus_tag="KPK67_001913"
     CDS             10454..11800
                     /gene="rpoN"
                     /locus_tag="KPK67_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBG2458882.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            12598..13620
                     /locus_tag="KPK67_001914"
     CDS             12598..13620
                     /locus_tag="KPK67_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2458883.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            13680..14687
                     /gene="gap"
                     /locus_tag="KPK67_001915"
     CDS             13680..14687
                     /gene="gap"
                     /locus_tag="KPK67_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG2458884.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            14909..16111
                     /locus_tag="KPK67_001916"
     CDS             14909..16111
                     /locus_tag="KPK67_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBG2458885.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            16137..16880
                     /locus_tag="KPK67_001917"
     CDS             16137..16880
                     /locus_tag="KPK67_001917"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBG2458886.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            16889..18421
                     /locus_tag="KPK67_001918"
     CDS             16889..18421
                     /locus_tag="KPK67_001918"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBG2458887.1"
                     /translation="MMKKTVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         7521 a         2160 c         3760 g         5282 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggttatatg atgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctacgcaggt
      181 gaggctggaa taagaactat aggatttggt ccttctaagg aaaatcttgc tcatacaata
      241 gatgagtata tagaattaga ccaattattc aaaggtgcag aaggatatta tggtatatta
      301 aaatcagttt ataaaaaata attaggagat ggttttgtga gaactttaat aaaacatggg
      361 cttatagtag atggaaataa aacaccagca tatgaaggag atattttaat agaaaacgag
      421 aaaatattaa aaatatcaca agatttaaac gaagaagctg acaaagttat agatgcaaaa
      481 ggtagagtta tatgtccagg tttcatagat actcatagcc attcagacct agttatttta
      541 gttaatccat ataatgaggt aaagattaga cagggaatta caacagaagt gttgggccaa
      601 gatggtatat ctatggcccc tctacctcaa gagcatatat cttcttggag aaaaaattta
      661 gcaggtctag atggtgaaag tgatgaaatt gattggaaat atgaaacaac agagaattat
      721 ttaaaaatga tggattacaa tggagtaggt ttaaatgaaa cttatttagt acctcatgga
      781 aacgttagaa tggaagctat gggacttgaa gatagacctg ctactaaaga agaaattcaa
      841 aaaatgtgtg aaatcacaga gagagaatta aaagcaggag ctataggatt gtctactgga
      901 cttatatata taccttgtgc atactcattg actgaagaaa ttatagagat gtgtaaggta
      961 gtagcaaaat atgacggagt atttgtagtt caccaaagaa gtgaagcaga tacaatttta
     1021 acttcaatgg aagaaatcat agaaattgga aaacaatcag gagtaaaagt tcatttttca
     1081 cattttaagg tttgtggtaa agcaaactgg aagtatatac cacaagttat agagctttta
     1141 gaaaaagcag aaaaggaagg aataagagta tcttttgacc aatatccata tgcagcagga
     1201 agtacaatgc ttggagtagt tttacctcct tgggcacatt ctggaggaac tgataagtta
     1261 atagaaagat taagtgatga aaacgaaaga gcaaaaatga aaaaagatat agcaaatgga
     1321 atagaaggct gggataattt tatagaattt gctggaatag accaaatatt tgtaacaagc
     1381 gtaaaaacag aaaagaataa agatacaatt ggtaagagtt tattagaaat tggaagaatg
     1441 agaggaaaag acccattaga tgcaacgttt gatttattaa aagaagaaga aaatgcagtt
     1501 gggatggtag atttctatgg attagaagaa catataatag gattcatgaa aagagacgag
     1561 caaaatgtgt gtacagatgg tcttttagca ggtaaaccac atccaagagc ttatggttct
     1621 ttccctaaaa tactaggaag atatgttaga gagttgaatg tgttaacaat cgaagaagct
     1681 gtttataaaa tgactaaaaa ggcagctaca agctttagta taaaagatag aggcgaatta
     1741 aaagaaggat attttgcaga tatagttata tttgacaagg atacagttag tggttgtgat
     1801 gattttatca actctatgca gtaccctaca ggtatagatt acgtaattat aaatggaaat
     1861 tgtgttgttg aagaaggaaa atacaataaa attaaagcag gaaaagtact taaaaattaa
     1921 atcttagctt taaggaggaa taaatttatg attttagtgg ggaatgggag gctaataagc
     1981 caagattctt taaatcccta tatggaagat ggatgtgtag taataaatga taatctaatt
     2041 gaagatatag gtactacaga agaaatgaaa gctaaatatc cagtacatga atttatagat
     2101 gcaggcaaga aaataatcat gccaggtcta ataaattcac atatgcatat atacagctca
     2161 tttgctagag gaatggcagt tccaggaaaa ccttctgaga actttttaga gatactaaac
     2221 aatttatggt ggagacttga caaacagctt acactggaag atacaaaata cagtgcatat
     2281 gcaacgtata tagaatgtat aaaaaatggt gtaacaacag tttatgacca tcatgcaagt
     2341 ccaaatgcaa ttgaaggaag tttgtttaca atatcagatg ttgcaaagga tttgggaatt
     2401 agaacgtgtc tttgttatga agtgtctgat agagatggtg gaaatacaat ccaacaaggt
     2461 atcaatgaaa acgtaaactt tataaaatac gctcaaaaag atgatacaga catgataaaa
     2521 ggaatgtttg ggcttcatgc tgcatttaca ctatctgatg atacattaaa taaatgtgct
     2581 tcagagatgg ctggtcttga tacaggttac catgtacatg ttgctgaagg actagcagac
     2641 ttacaatata atgtagataa atatggaaaa agggttttag aaagattcaa tgattttgga
     2701 gtactaaaag aaaaaacaat agcagttcat tgtatatata taagtgcaga agaaatcagt
     2761 cttttaaata aaacaaatac atttgtagtt cataatccag aatctaatat gggaaatgca
     2821 gttggatgtt ctcctgcaat agagttattg aaaaggggag ttacagtagg attaggaact
     2881 gatggatata ctagcgatat gtttgaatca ttaaaagttg ccaatataat tcacaaacat
     2941 cacttaaaag acccaagagt tggatttgaa gaatcacaaa caatgttatt tgataacaat
     3001 agaaaaatag ctaaagctta ttataaaaat gacctaggag tacttaaaaa aggtgcttat
     3061 gcagatgtta tagtagttga ttatatacca catactccat tgacagaaaa tacaataggg
     3121 ggacacacta tttttggaat gagtggaaga agcgtagata caactattat aaatggaaaa
     3181 gtacttatga aagatagaaa gttaataaac atagatgaag aaagtattct agcaaaaagt
     3241 agagaattaa gtcaaaatct atggaataga atttaaataa ttgatattaa tatacaagta
     3301 agaaacaaga atttagatgt taaaaacaga gcttaaacaa aaataggaat ttaaatgaag
     3361 tatataaata aaaaatatac ggaatagaat attaaattaa atatagaagg gagacccaaa
     3421 atgtctcgag ataaaagccc taaaatagct ccagtagatg aaaggttacc tgtatccaaa
     3481 tcttgggtat tttcactaca gcacgtttta gcaatgtgtg caggagcagt agcagttcct
     3541 atgatgatag gaagtgctgc acgactagat catgctagta ttgttttttt aataaatgct
     3601 tgcttgttta tggcaggtat aggaacactc atacaaagtg ttggtatagg aaatattgta
     3661 ggtgctaaaa taccagtaat agaaggaact agttttgccg ctgtggcagg tatgacagca
     3721 atagcctcat catatagtga ttcacaagtt gcaatacaga cagtatttgg ctctgtaata
     3781 gtagctggat tgttttgtat attacttgct ccagtatttg aaaaattaat aagatttttc
     3841 cctagtgtag ttactggaac agttgttatg gtaataggta tttcactatt tccagttggg
     3901 ataaaatgga taactgacaa caaattggaa gcggttgaac caaactcatt agcattagct
     3961 ttagcagtat tggcaataac tttactttca tttaaattct taaaaggaat ttggaatagc
     4021 acagctatat tggtaggtat agtacttggt acactacttt ccatggcagt tggaatggca
     4081 gattttagtg cagtaaatgg agttggttgg cttaacttaa atgtaccttt aaaatttgga
     4141 atgcctagat ttgaaccatc tgcaatacta tcattgtgtt tgattatgtt ggtacttatg
     4201 acagaatcag ttggtaatat gatagctatt catgaaatgg tagacaaaga agtaacaggt
     4261 aaaaatatta aaagaggtct atttggagat ggaatatcta cagcattatc tggaatattc
     4321 aattcatttc caataactcc attcggtcaa aatacaggaa tagtaggtct tacaggtata
     4381 aaatcaagat ttatagcagt ttacgcaggg gttatattgc tagtacttgg attcttacca
     4441 aaatttgcag caataatagg agcaatacct aagcctgttc ttggaggcgt aggatttgca
     4501 atgtttggaa tggtaatggt tggaggagta aaaactttaa gcaaagtgaa atttgatgga
     4561 actaaaaatg gggttatagt agcagttagt gttggtttgt ctatgatacc actaacaaat
     4621 cctgatttct atcataattt cccaacttgg gtacaaacaa tattccatag tggaataaca
     4681 acaggtagtt tatcagcagt gcttttaaac atattcttca atgaactagg ttctaaaaaa
     4741 gaaaaaaata ataaagttga agaagcaaaa taaaaataaa acaagtaaaa atctatattg
     4801 acttaaagtc attaagcatt aataaaaata attcagagat attaataatt caacaaaact
     4861 atcttatatt ttaaataata taaaaaagta aataaataac gaatacaaat aaaagaatac
     4921 aatctaaata ttaaaaatat ttaatttaag ggagagataa taatatggct aatttaaaag
     4981 ttaatttata tggaatggaa tttttaaacc caattatgac agcggcagga cctggagcaa
     5041 aagatggtgc tctctgtcaa gaggcagcaa aaggtggagc aggagctata tgtacaaaaa
     5101 ctatatctgt attaccagct gatgtaccaa gaccatgtat ggcaaaaact aatagtggtt
     5161 ttttaaacac agaattatgg tcagagcttc caaaagagca atggatagag gaagaataca
     5221 aaaaggtaaa agaaacaggg cttccagtaa tagtaagtat gggatattca gcagagcaaa
     5281 tagctgaggt tgctccttta gtaaagcctt atgcagatgc agttgaatta tctactcatt
     5341 atgtaggtac agacgttgca ccaatagtaa atgctttaaa agctgcaaaa gctgttttag
     5401 atgtaccagt atttatgaaa atgagtcctc atactgacat tcaaactatt gcaaaagctg
     5461 ttgaagaagc aggtgcagat ggacttgtaa tgataaactc atttggacca tgtatggcaa
     5521 tagatgtaaa tacaggatac cctataatgg gaagtaaagc tggatacgga tggttatcag
     5581 gtgcaccaat aagacctcta gcaataagat gtatatatga agcttctcaa gttgtaaata
     5641 taccaataat cggtgttggt ggaatcactt gtggaaaaga cgtggctgaa atgatgatgg
     5701 ctggagcaag tgctgttcaa gtatgtacag aagcaatcct taaagggcct tctatctatg
     5761 gaaaaattgc taaagaatta aatgaatttt tagatgagaa tggatatgct gatgttaatg
     5821 aaataaaagg attaactcat aagaaaatgg cagctagagg gtttagaact cattcagtag
     5881 ctccagtagt ggatgataac aagtgtatca aatgtaagca atgtgtaaca agctgtgtat
     5941 acgaagcttt agaagttaca gataagttga acatagatgc agacaagtgt tttggatgtg
     6001 gattatgtgt aactagatgt ccaaaaggag ctttaagcat ctgctacaat caagtagaag
     6061 ctatgaatga gtaataatta tatagaattt tggtaattaa cttagagtaa taggtattag
     6121 gataataaaa tatatcagat aaacaaacac acattatgca ttaataatac agtctaaagt
     6181 taataatttt attatattga tttaactaaa tattagttta gctgaatatt aatttaatta
     6241 ggtattggtt taatcttaat atagatttac tacgtataag tgaactaact agattacttt
     6301 aagttaattt tttcatataa aataaattga tacattacaa gtaaggtgga gtctatatgg
     6361 gaattgtttt aaaaggtggg aaagtagtaa caagtgaaaa aacttactgt gcagatgtaa
     6421 gaatagaaga tgaaaaaata gtcgatatag gtcttgatat atcaaaagaa aatgatgaaa
     6481 tagttgatgt tagtgattct tatgttatac caggaggtat agatacacac acacactttg
     6541 acttagatac aggcgttact aagacagcag ataactttga gacaggaaca aaagctgcta
     6601 tagtaggagg aactactact atattggatt ttgctacaca agctaaagga tgttctttga
     6661 aagacggatt agaagaatgg catgaaaaag ctaaggataa atcctattca gattatggct
     6721 ttcatatggc tataacagat tggaatgact cggtttgtaa tgaaatggag gatatggtga
     6781 aagagggagt atcttcgttt aaactgtata tggcgtataa aggaagcctt caagttgatg
     6841 atggtgtaat ctttgaagca ttgagaaaag cagaagagat aggaggtatt attggtttcc
     6901 attgtgaaaa tggggacata atatgtgaac ttgtggataa agcaaagtca gaaaataatt
     6961 tatctccaaa atatcatcaa ttaactagac cagctataat ggaggcagaa gctacatcaa
     7021 gacttatgaa gatagctgaa gttgcaaact ctaaagtata tgtagttcac ttaagctgta
     7081 aagaatcctt agaggaggtt ttaaaagcta agcaaagagg tgtggatgta atagttgaaa
     7141 cttgtcctca ataccttcta ttaaatgatg aattatatga tttagatgga tttgaaagtg
     7201 ctaagtatgt aatgtcacca cctttaagaa ataaagaaaa taatgaaata ttatggaatg
     7261 ctattttaga taatcaaata gacactatag gaacagacca ttgttctttt aattataagg
     7321 gacagaagga acttgggata aacgatttca gtaagatacc aaatggttct ccaggtgtag
     7381 aacataggat gagtctttta tacacatatg gagtctgtaa aaataaaata tctatcaaca
     7441 agatggtaga agtaacatca actaatgcag caaagttgtt tggaatgtat cctaataaag
     7501 gaactataga aataggcagt gatgcagata tattagtctt aaatccaaac aaaaaatcca
     7561 aaatagaagc aaaaaaacaa ttacaaaatg tagattatac accttatgaa gggtatgaag
     7621 tggattgtca aatagaacat gtttatttaa gaggaaaaga agtagttaga gatggagaat
     7681 taatttgtaa tactccaagt gggagatata taaataggcg tctaacaaag ggggctagag
     7741 aatggtagaa tttatattaa atggagagaa agttgttgca aaagaaggca aaaaactttt
     7801 agacttcctt agagaagata tgaatataac ttcagttaaa aatggatgtt cagaaggtgc
     7861 ttgtggaaca tgtatggtta taatagatgg taaagctact aaagcatgtg tatttaaaac
     7921 tgataaatta gctgggaaaa acataacaac tatagaggga ttatcagaga gagaaagaga
     7981 tgtttttgca tattcttttg ctagtcaagg agctgtacaa tgtggatttt gtattccagg
     8041 tatggtaatt agttcaaaag cacttataga cgttaataat aatcctactg aagatgatat
     8101 aaagaaagct ttaaaaggca atatatgtag atgtacaggg tatgtaaaaa taattaaagc
     8161 tgtcaattta gcggcaaaga tttttagaga aaatatagat gtgccaaaag taaactgtaa
     8221 gggtctagta ggtgaaaatc ttcctagagt tgatgcagtt ggcaaagcac ttggatattc
     8281 tgaatatgta gatgatatga gaattgaggg tatgatttat ggttcagcag ttagagctaa
     8341 gtatcctaga gctttagtta aaaaaataga tatagaagaa gcaaaagctt atccaggagt
     8401 tgttgcagtt ctaacagcta aagatattcc aggaactata aaggtagggc acttaaagaa
     8461 ggattgggat actttaatac cagaaggtga aataactaga tacttaggag atggaatagt
     8521 tttagtagca gcagaaacta gagaagcgtt agaagaggcc aaaaagctag ttaagataga
     8581 ttatgaggaa ttaacaccac taagtactac agcagatgca cttagagaag atgctccaaa
     8641 gatacatgag acagggaata tacttgttaa ggaacattta gtaagaggaa acgctaaaga
     8701 aaagatagaa aattcaaaat atgttgtaac taaaagatat actacacctg ctacagaaca
     8761 cgcattttta gagcctgaat gtgcagtagc tggtccttat gaagatgatg gagtaataat
     8821 ctattcaaca gaccaagggg tgtttgccac tcaacatgaa tgtgcagata tgttagggtt
     8881 accatatgag aaggtaagag ttataaacaa aatggtagga ggagggtttg gtggaaaaga
     8941 agacatgagt gttcaacacc atgcagcctt acttgcatac catacaaaga gatttgtaaa
     9001 agtacagtta acaagaaaag aaagtattat tgttcatcca aaacgacatg ccacagaaat
     9061 ggaatttacg acagcatgtg atgaaaatgg atacttaacg ggtatgaagg caacgataat
     9121 atctgacaca ggtgcatatg cctctcttgg aggaccagtg ctacaaagac tgtgtactca
     9181 tgcagcagga ccatacaatt atcaagatat tgatataaca ggaacagcag tatatactaa
     9241 taaccctcct ggaggagcat ttagaggatt tggagttaca cagagttgtt ttgcaactga
     9301 atgtaatcta aatttacttg ctgaaatggt tggaatatct ccttgggaga taagatataa
     9361 aaatgcaata agacctgggc aagttttacc aaatggtcaa tttgcagatg aaggaacagc
     9421 aatggcagag acactagaag ctgtaaaaga agagtatgaa aagcatccac gtgcaggtat
     9481 agcttgtgcc atgaaaaata gtggggttgg agttggtata cctgatgttg gaagatgtag
     9541 attggtgata aaagatggca aagttcacat aagaacaagt gcttcttgta taggtcaagg
     9601 gcttggtact gttatgactc aagtagtttg tgaaactgct aacctacttc ctgaaaatat
     9661 agtgcatgaa gttccagata ctcatttaac accagactct ggtaatacta cggcttcaag
     9721 acagacagta tttacgggag aagctactaa ggttgcatca aataagttaa aagaagcttt
     9781 agactctggc aaaactttac ttgatttaga gggagaagag ttttatggag aatatgaaag
     9841 tattacagat aaaatgggtt gtgacaaaga aaatccagta agtcatgttg cttatggata
     9901 tgcaacacaa gttgtaattc tggatgaaaa tggaaaacta gaaaaagtag tagcagcaca
     9961 tgatgttgga agagctataa atccaatcag tttagaaggt caaatagaag gtggagtagt
    10021 tatgggtctt ggatatgctc taaccgaaga ttacccagta gtcaactctg ttcctacagc
    10081 taagtttggt acattaggtt tatttaaatc tacagatata ccagaaatac aagctttaat
    10141 agttgaaaag aacaaagatt tattggctta tggggcaaaa ggtgtaggag aaatttgtac
    10201 agttcctact gcaccagcag tacaaaatgc ctattatgta tttgataaag aatttagaac
    10261 tgagctacca ctacaaaata ctccatatag taaaaaaagt aacaaaaaaa agaaatatta
    10321 aataaaaaaa tagatgaaat tgtaaataaa aaaaaccctt ttgcataagg tatattttaa
    10381 atgatagaat atacatatac aggagggttt ttcttttata aaataagttt aataaaattg
    10441 agaggtgagc agaatgaatt ttaataatag tctagaatta actcagtctc agaagctaat
    10501 aatgactaca caacttaagc aatcattatc aatattaaat atgagtaagt tagagttaga
    10561 agaagagata aaaagagaag cagagaataa cccattagtt gaagtcgaga aaagtgggga
    10621 gataaattgg gaagagtata taaaagacat ggataggtca agacgtttag atagaacaga
    10681 gataagctat aatcctgata atgaagttaa tctagaaaat ttagttaaat attcgtcaaa
    10741 tttatatgaa gatttaaaat ttcagataag cctgtataaa cttacagaca aagaattgga
    10801 agtctgtgag tatataattg atagtttaga tgaggatgga tatttaagaa ctgaggaaaa
    10861 agtaattgtt gataccttaa agatagatga agaacttttt gaaaaatgtt taattagtgt
    10921 tcagcagttg gaacctagtg gagtaggggc aagaagtctt tcagaatgtc tgatgataca
    10981 aatggctagt ttaggaatat ataatgaaat tttagaggaa atcgttacaa aagacttaaa
    11041 tttaattgca aataataaat acaaagagat aagtaaaaag tacaatatgt cattacaaaa
    11101 atgtgttgaa ctaataaaca taattaagac attagacccc aaaccaggaa gaacttgttc
    11161 agtagagaaa agtgtataca ttcaacctga tgtaacagta gaaaaaattg atgatgaatt
    11221 tattgtatat ttaaatgaga aagattctta tcaaataaga atcaatagtt attataaaga
    11281 catattaaaa aattcacagt ctgatgaaag tgcaaaagaa tttataaaag aaagactaaa
    11341 ttcagcaaca ggacttatga aaaatataga gagtaggaaa actacagtac ttaagattgc
    11401 agaagagata gtaaaagctc aagatgaatt tttgagaaaa gggacaaaat atataaaacc
    11461 tttgaaaatg aaagacatag cagaaaaatt agaatttcat gaatctacta taagtagagg
    11521 tgtaaatggt aaatatatgc taacaccttt tggcgtatat gaatttaggt actttttcag
    11581 tagcgccatt gagactgaga ataatgaaat ggcatctagt acaagcataa aaaaaattat
    11641 aaaggaaaca ataaaagacg aaaataaaaa gaaaccttta agtgatgacc atatatctaa
    11701 gttactaaaa gaaaaaggaa tcaatgttgc aagaagaaca gttgccaaat atcgtgaaga
    11761 attaggaatc ccatcttcaa gtaagagaaa agaatactaa taagtaaaag acaaatatca
    11821 gtatggtaag gggaaaaggc taattaagag atgaatgtag attgggctaa aaattttata
    11881 aagtaaaata atagaatatg gaaaagtatt aaaatgctat aaggtattaa aaaactgggg
    11941 aacattaaaa cattaagata gcaaagaaca taaaaaacta aagaagatta aaacattaaa
    12001 gaacattaga gctttgagga atattagaac tttaaagaat attaaaattt ataaaatata
    12061 aaaatactaa aaagaattga aaaactgggg aacattaaaa tattgtaaaa tgctaaaaaa
    12121 ctagaaaaca taattaaata agtgcttata aagaatgatt aaattaaata agtgtttgta
    12181 aagaataatt agatattaga aaaagaagaa tacctagata aataaactat tattaaattt
    12241 aataatcaca aaaggaaaac acgtagggta taaataaaaa tcttaaatag ataaaagtaa
    12301 acttagttgt agataaaaat aactagaaat aaaagtaact agaaataaaa ttatttatag
    12361 taataaatat tagaatgaaa atataaaaaa atatatataa aaagccttat ataaaaagaa
    12421 aaaatactgt gatttgaaaa tggtggagaa atattttata aaataaaaaa ataaaaaagt
    12481 ttttgaaaaa ctattgaaat ctatacgcaa atcctataaa ataaaaatat cttgtgggac
    12541 ttaaataaac actagggaca aaaaaagtcc cgaaaggaaa gcaataagga gtatgttatg
    12601 aagaacttat tagaaattca aaagaaattg attccacagg caattgaact tatggaaaga
    12661 agatactcta ttttaagaca aatatcttta agtgaaccaa taggtaggag aacattatca
    12721 aatgtattag agattagtga aagaataatc aggtcagaaa ctgagttttt aaaagaacaa
    12781 ggacttatag acgtagctgt atctgggatg actatcacta aagaaggttc tgatttgtta
    12841 gataaactta aggacatcat gagtgacatc atgggattgt ctaagttaca agatagagtc
    12901 agagaaaagc ttggaataaa aagggtaata ttagttcctg gaagctgtga tgaaaatgga
    12961 agtttactaa aagatgtaac tagatatgga tgcgaatatt tcttaggcat tttaaaagat
    13021 ggagatatcg tttcaataac aggaggaagc actatgttgg agttttcaaa cgttattaaa
    13081 actgataaga gatatccaaa ctctacaata gttccagcta gaggaagtat gggaactgat
    13141 gtagagatgc aatctaacaa tatagttgct tcaataagta agaaattaca ttctaattgc
    13201 aaacttttac atataccaga tgagttggat gaaagtgcaa tgaaaactct tagccaagtg
    13261 ccagagatta agacaacttt ggagtatatt aaaagaacag acattttagt ttttagtata
    13321 ggtagagcag atgtcatggc aaagagaaga aaacttccag aagacaaggt tgatgagata
    13381 ttatctaaaa atgcagttgg agaagcattt ggatattatt tcaacaaaga tggagaaata
    13441 gtttacaaac tgaacactgt tggaattgat ttagaaacag ttaaacaagt aaaagaaact
    13501 attgcaatct ttgcggggac tgaaaaagta gaagccttat tagctatatc aaatatgcat
    13561 aaaaacatgg ttcttgtaac agatgaagaa agtgcttata agatattgtc attattataa
    13621 aagtcaagac ctagcacaga gctagaaaac atacatttac tttataggag gtagcgaaaa
    13681 tggttaaagt agctataaat ggatttggaa gaataggaag attagcatta agaaaaatga
    13741 tggagcaaca agataagttc gaagttgttg caataaacga cttaacagac gctaaaatgt
    13801 tagctcattt atttaaatat gatacagctc aaggaagatt taacggagaa atagaagtta
    13861 aagaaggtgc ttttgtagta aacggaaaag aaataaaagt aacagcagaa agaaatcctg
    13921 ctgacctacc atgggctgaa ttaggagtag atatagtatt agaatgtaca ggattcttta
    13981 catctaaaga taaagctgaa gctcatatac aagcaggggc taagaaagtt gttatatctg
    14041 caccagctac tggagattta aaaacaatag tattcaacac aaacagtgac atattagatg
    14101 gttctgaaac agttatatca ggagcttctt gtactactaa ctgtttagct ccaatggcta
    14161 aagtattaaa tgataaatac ggtatagaaa aaggattaat gactactata catgcataca
    14221 ctaatgacca aaatacttta gatggtcctc atccaaaagg tgatttaaga agagctagag
    14281 ctgctgctgg aaacatagtt ccaaacacta caggagctgc aaaagcaata ggcttagtaa
    14341 taccttcatt aaaaggaaaa ttagatgggg cagcacaaag agtaccagtt gtaactggtt
    14401 caataactga attagtttgt acattaggaa aaaatgttac agttgaagaa ataaatgcag
    14461 ctatgaaaga agcttcaaac gaatctttcg gatatactga agaaatgtta gtatcttctg
    14521 acataatcgg aataagctac ggttcattat ttgacgctac tcaaacaaaa gttatggaag
    14581 tagatggaaa acaattagtt aaagttgttt cttggtatga taatgaaatg tcttatactt
    14641 ctcaattaat aagaacttta ggatactttg cacaattagc aaaatagtca gttttgagaa
    14701 aatagaaaag tagattgaca aagtccgagt ttgtagttta atactacgga ctcaggactt
    14761 tttttaagaa aaaatatata aaatacacct aaataatgtt acatactata tataaaaata
    14821 tgataatatg atatatttaa tagggtaaaa gtatgtacta tattatactt ggcaataaaa
    14881 aattacttta ttaaaaggag agatatttat gtcaatgctt aacaaaaaaa ctatagaaga
    14941 tattgatgtg tgcggaaaaa aagtattagt aagatgtgac tttaatgtgc cattacaaga
    15001 tggtgtaatt actgatgaaa atagattgaa cggagcttta ccaactattc aatatttaat
    15061 ttctaaaggg gcaaaagtta tattatgctc tcacttagga aagccaaaag gagaagctaa
    15121 accagaatta tctttagcac cagtagcaaa gagattatct gagatgttag gaaaagaagt
    15181 tgtatttgca gcagacgata atgttgtagg agaaaatgca aagaaagcaa ctgaaaaaat
    15241 ggaaaacgga gatgtagtat tattagaaaa tactagatat agaaaagaag aaactaaaaa
    15301 cgaagagaat ttctctaaag aactagcatc acttgctgaa atatttgtaa atgatgcatt
    15361 tggaactgct catagagcgc attgctcaac agttggtgca ggcgagtttt tacaagagag
    15421 agtttgtgga tacttaattc aaaaagaatt aaaattctta ggagaagctg ttgcaaaccc
    15481 tgtaagacca tttacagcta tacttggagg agctaaagtt tctgataaat tagctgttat
    15541 aaacgaatta ttagaaaaag ttgataatct aataataggt ggaggaatgg cttatacatt
    15601 cttaaaagct cagggttatg aagtaggaac ttctttactt gaaatagata aagttgaata
    15661 tgctaaagaa atgatggaaa aagctaagaa taagggtgtt aacttattat taccagtaga
    15721 cgttgttatg gcagaccact ttgcaccaga tgctactcca atagtaacag aagatgctaa
    15781 tgtaaaagaa gattacatgg gactagatat gggacctaag actatagcaa actttgtaaa
    15841 aactataaaa gaatcaaaaa ctgtagtatg gaatgggcca atgggagtat ttgaatttga
    15901 aaactttgca aatggtacat tatcagtagc tagagctatg gcagagttga ctgatgcaac
    15961 tactgtaatt ggcggtggag atagtgcagc agcagttaat caattaggat ttggtgacaa
    16021 gatgactcac gtatcaacag gtggaggagc atcactagaa tttttagaag gtaaagaatt
    16081 accaggtatt gcagcattag ataataaata aactaatata tagaggagat tgtaaaatga
    16141 gaaaacctat aattgcagga aactggaaaa tgcataaaac aataaaagaa gctttagaat
    16201 ttgtaaatga agttaaagat aaagtaaatt ctgataaagt agaagctgtt atatgtgcac
    16261 catttacttt attaaaggat ttaaaagaag ctactaaggg tacaaatata aagataggtg
    16321 ctcaaaatat gcactttgaa gaaaaaggag catttacagg agaagtttca cctcttatgt
    16381 taaaagaaat agatatggac tatgttgtaa taggacattc agaaagaaga caatacttca
    16441 atgaaactga tgaaactgta aacaagaaag ttttaaaagc attagaagta ggaatagacc
    16501 caatattatg tgttggagaa actttagaac aaagagaagc tggaaaaact aaagatgttt
    16561 gtaaagttca agttgaaaaa gctttagaaa atgttcttaa agatgattta gcaaaagtag
    16621 ttgttgcata tgagccaatc tgggctattg gaactggtaa aactgcaact gctgaagatg
    16681 ctaatgacgt tatatcttat ataagagaag ttataaaagg attatatgga gaattagcta
    16741 atgaagttag aatacaatac ggtggaagtg ttaaaccttc aaatgtagcc gaaataatgg
    16801 gtcaaagtga tatagatgga gctttagtag gtggagctag tttagctagt aatgattacc
    16861 tagaccttgt taatttctaa ggagtgaaat gatgaaaaaa acagttgctt taattattat
    16921 ggacgggttt ggatacaata aggatgttaa gggaaatgca attgctgagt ccaaaactcc
    16981 taatttagat agaataaaaa aagagtatcc aaacacattg ataaatgcta gtggtcttga
    17041 tgttggtcta cctgatggtc aaatgggaaa ttcagaagtt ggacacacta acataggtgc
    17101 aggtagaata gtatatcaag atttaactag aataacaaaa tctataaaag atggtgactt
    17161 ttttactaat aaagttttat gtgaagcaat ggataatgca aaagaaaatt ctcttcatgt
    17221 aatgggactt ttgtctgatg gaggagtaca ttcacatata gaccatctta aggctataat
    17281 caaaatggca aaagataaag gcgtacaaaa ggtgtatgtt cacgcattta ctgatggtag
    17341 agatactgac cctcaaagtg cattagaata tgccaaagaa gtacaagcta gtatggatga
    17401 aattggagta ggtgaatttg ctacagtttc tggtagatac tacgctatgg atagagataa
    17461 gagatgggaa agagtagaac ttgcatacaa tgccatggtt agaggtattg gtgaaaaagc
    17521 aaattctatt gaggaagcta ttcaaaactc ttatgatgat ggaaaaaatg atgagtttat
    17581 aatgccaaca gttattatga aagatgataa accagttgga agtataaagg aaaatgattc
    17641 aattatattc ttcaacttta gaccagatag agctagacaa ataactagag ctttagtttg
    17701 tgaagagttt gatggattta aaagagaaga tattaaaaac ttctttgtat gcttaacaga
    17761 gtacgatata actatagaga atgtgcatat agcatttgga cctcaatctt tagccaacac
    17821 tctaggtgag tatctagcta aaaatggaaa aactcaactt agagctgctg aaactgaaaa
    17881 gtatgcacat gtaactttct tcttcaatgg tggagtagaa gagccaaata aaggtgaaga
    17941 aagactattg ataccttctc caaaggttgc aacttatgat ttaaaaccag aaatgtcagc
    18001 atatgaattg acagataaag ctttagataa attaggagaa gataagtttg actttatagt
    18061 attaaacttt gcaaatcctg atatggtagg tcatacagga agtatagaag cggctataaa
    18121 ggctgttgaa actgtagata cttgtgtagg aaaattgata gataaaatag tagagttagg
    18181 tggaagtgca ataataactg ctgaccacgg aaatgctgaa tatatgttag acccagaaac
    18241 aggaaaaact gtaacggctc actctattaa tccagtacca tttattgtag ttggtcaaga
    18301 atatgaaagt gcaaaattat tggatggagg aagattatct gatatagcac cgactgtttt
    18361 agatatgatg aagttagaaa agccagaaga aatgactggc cattcactta tttcaaaata
    18421 gtcgattctg ataagtagag aataatatat aaactacaat ataaatttaa aaataatgca
    18481 gtttaggttt attttgtaaa gtttagactt tatgacataa gatttagatt ttgtaagttc
    18541 tggatattgt aaggtgtaga tgaagatata ttttataaaa tttacaagat agacaattta
    18601 taaaataaaa aattacaata tagataataa tgtaaataat gatataaata ataacaagat
    18661 agacaataac aatataaata ataataatta atatatatta tataagcaaa aattttagaa
    18721 gca
//
LOCUS       DAEMHW010000067        18499 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEMHW010000067 DAEMHW010000000
VERSION     DAEMHW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18499
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.067"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..360)
                     /locus_tag="KPK67_001919"
     CDS             complement(<1..360)
                     /locus_tag="KPK67_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2458888.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKE"
     gene            927..1289
                     /locus_tag="KPK67_001920"
     CDS             927..1289
                     /locus_tag="KPK67_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458889.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1273..1989
                     /locus_tag="KPK67_001921"
     CDS             1273..1989
                     /locus_tag="KPK67_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458890.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVRGKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEIGGFTEEDEEDEEELKQNIIE
                     NIKKNFDEKNLVQVYKYRGV"
     gene            2009..2428
                     /locus_tag="KPK67_001922"
     CDS             2009..2428
                     /locus_tag="KPK67_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458891.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2418..2942
                     /locus_tag="KPK67_001923"
     CDS             2418..2942
                     /locus_tag="KPK67_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458892.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYVDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKMKVWNFSESI"
     gene            3254..3811
                     /gene="efp"
                     /locus_tag="KPK67_001924"
     CDS             3254..3811
                     /gene="efp"
                     /locus_tag="KPK67_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBG2458893.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            3903..4358
                     /locus_tag="KPK67_001925"
     CDS             3903..4358
                     /locus_tag="KPK67_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2458894.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            4521..5231
                     /locus_tag="KPK67_001926"
     CDS             4521..5231
                     /locus_tag="KPK67_001926"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBG2458895.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5285..6355
                     /locus_tag="KPK67_001927"
     CDS             5285..6355
                     /locus_tag="KPK67_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2458896.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6383..9937
                     /gene="smc"
                     /locus_tag="KPK67_001928"
     CDS             6383..9937
                     /gene="smc"
                     /locus_tag="KPK67_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBG2458897.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKILEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLESSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINLEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            9964..11244
                     /gene="ftsY"
                     /locus_tag="KPK67_001929"
     CDS             9964..11244
                     /gene="ftsY"
                     /locus_tag="KPK67_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBG2458898.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKTEESVNNEGTEEIENFEKIEVEMNSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11347..11715
                     /locus_tag="KPK67_001930"
     CDS             11347..11715
                     /locus_tag="KPK67_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBG2458899.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            11712..13064
                     /gene="ffh"
                     /locus_tag="KPK67_001931"
     CDS             11712..13064
                     /gene="ffh"
                     /locus_tag="KPK67_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBG2458900.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13102..13374
                     /gene="rpsP"
                     /locus_tag="KPK67_001932"
     CDS             13102..13374
                     /gene="rpsP"
                     /locus_tag="KPK67_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBG2458901.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13439..13666
                     /locus_tag="KPK67_001933"
     CDS             13439..13666
                     /locus_tag="KPK67_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBG2458902.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIILKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            13774..14289
                     /gene="rimM"
                     /locus_tag="KPK67_001934"
     CDS             13774..14289
                     /gene="rimM"
                     /locus_tag="KPK67_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBG2458903.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14298..14993
                     /gene="trmD"
                     /locus_tag="KPK67_001935"
     CDS             14298..14993
                     /gene="trmD"
                     /locus_tag="KPK67_001935"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBG2458904.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15178..15528
                     /gene="rplS"
                     /locus_tag="KPK67_001936"
     CDS             15178..15528
                     /gene="rplS"
                     /locus_tag="KPK67_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBG2458905.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            15849..16745
                     /gene="ylqF"
                     /locus_tag="KPK67_001937"
     CDS             15849..16745
                     /gene="ylqF"
                     /locus_tag="KPK67_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBG2458906.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            17134..18426
                     /gene="brnQ"
                     /locus_tag="KPK67_001938"
     CDS             17134..18426
                     /gene="brnQ"
                     /locus_tag="KPK67_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG2458907.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         7823 a         1732 c         3425 g         5519 t
ORIGIN      
        1 ttctttatct gttccgatag ggtcttgtag agatacttga gttttatttt ttttgttaga
       61 tctaatattc attaaaatct cattttcaat acatctagaa gcatatgttg ctaatcgagt
      121 tcctttatct atgttataag tttctatagc tttaattaat cctattgtac ctatggatat
      181 taaatcatcc tgatcttccg ttgaattatt atatttcttt gctatataag ctactaatct
      241 catgtttctt tcaattaatg tatcttttgc acttttatcg ttttctattt taaatttagt
      301 taaatactca atctcttctt caggggttaa gggtttttca aaagatttaa gagctgccaa
      361 taggtcaccc ccttttcaac ggtcacttta aattatatga aggagactta tccaagttga
      421 ctgttatcta ataaaataat atgcatattt ttataattta aaacatatat ttgcaaactt
      481 ttgaatggaa tatgacataa agagtgggaa tttaaattta agtttttgtt gtatatatat
      541 gaaataaggc gaagaaatta ttgatttata aagtataaat tggaattgag gtgtcaataa
      601 atataaaagg aaatagtatt tatatatgtt ttggtgttga agtaaaagat taatgcaaag
      661 tattagagtc tataaaaata tgaatatagg aggtgtaatt ttacaatact taaaaaagta
      721 aatgtaatta attgtttgta taaagttgat tacaagcaaa agtatcagtc atagttaata
      781 ctgttaagga tgaaggtggt gtgttaattt atatatgtat gggaagaaat tttgattaca
      841 atgaaaaaac ttacaaaaga tatttacgaa aaaacttata gttaaatgtt tatatataaa
      901 atattattta ggggttgttt ttattattga aaataagaaa gagtggtttt atatcaatcg
      961 aatgtataat aagtattgct atattatatg tggctgttta tttagtttct acatcattgt
     1021 ataattgtta tagttttatc agtagaaata tatctgacag agaaatgtta agtacagcaa
     1081 aaaaatatat agaagatgag aagtatagaa tacaaaatag taagtatgag ttaattgaag
     1141 ataagataga aaaaaattac ataaatggat atgaaattaa cagtagaata gagcaaattt
     1201 tagattatta tcaatgctat gaaataaata tagagataaa aaatgaattt aaaaaactga
     1261 ggtttaatag ctatgttact agaaaataaa ggctctatat taataataac attgataata
     1321 ttttctatta taagtactgt gtgcattatg tgtataggtt taatttatac acataataat
     1381 atgtttgagt taggatacaa agacattcaa ctaaaagaaa tttctttaag tggaatagaa
     1441 atatcagtaa gtaatgtact ttcttgtgtt gaggaagcta ttgatgtatc taataatgaa
     1501 gacgaattta gagaatattt cttaggaaat aatatgattg aatgtataaa aaaaataaaa
     1561 gatacatctt atagtagttt agaagaactt tctttaaaag tagaaaataa tactatttat
     1621 gacagaggag atttttttga atttgatata gaaagtaatg ttagaggaaa agaattttta
     1681 aaagcaataa aagtaagagt gaaaatcaaa aatccatgga taggaattga cattgaagat
     1741 tatatggata aatctaataa caataaaatt gatgacaaaa cattcaataa ttataaagca
     1801 tctgaaaata atgaaattct aaatcaattt aaaaatgaaa gtgaaaatat aaggaatcaa
     1861 gagattggtg gatttacaga agaagatgaa gaagatgaag aagaacttaa acaaaacata
     1921 attgaaaata taaagaagaa ttttgatgaa aaaaacctag tacaggtata taagtatagg
     1981 ggtgtataac tttgaaaaaa aaaggaatgt gactgtaata gaattaatag tatctatcag
     2041 tataatactc ttgatagtta cattatcttt tccaaaagat aatcttgaaa atcataaaat
     2101 aaatttattt gcacgtcaac tttgttcaga tataaggtat gttaggcgaa ttaatatgtt
     2161 tagcaactac aatacatata tatattatat aaatcaaggt aaacacgaag gttatgtatt
     2221 gagaaaagat ggtaaaaaca ttaaatcaat aatgctacct aaaaatgcaa aaatagctag
     2281 gagtattgaa ataataaagt ttaaatctga tgggtcgcct caaaaggcgt ctacaataga
     2341 gatatataat aatagattaa gaaaaactat aacaataaca cctgtaagtg gaagagtact
     2401 tctgaaagag ggaaaatatg aaacataaat atggttactt gcttttggaa agtgtagtat
     2461 cattgtcttc aatggtgata attatactag ttttatattc tatatttttg tctacaataa
     2521 acttaaagtt aaaagtagaa gataagatag aactacaaca acaaagtctt gaaatcataa
     2581 aatctatgga aggaattatt agcaattcta tgggaataat gaatgtatct aattatgaag
     2641 aaacatttaa aaagacaacg tctataaaat gtagatatgt agatgaaaat aataatgaag
     2701 aaggcataag taataaagaa attatattga atgaaagacg aaataagtta tttgtaaatt
     2761 ctttaaatgg cgaaagtagt caagcaggtg gttatgaaat tggagattat gtagatgaga
     2821 tgtatgtatc aattactaat aatggtcaat atgttaatat taaattgaaa ttatcaaaac
     2881 gtagtcaaaa atatgagact gatttcaaaa tgaaagtatg gaattttagt gaaagtatat
     2941 aaaaatacat aaatttaaga gaaagaagtg acatcaataa gtcatataaa atataattat
     3001 tgagaactat ataactaaat aatattaata tgtaaagtgt atagattaag aatatagagg
     3061 ataataataa aaatatagaa taagtaattt gccaaatact tgtttatatt gtataactta
     3121 tgatattatg atattagtaa aaatttgtaa tcctgacttg acagaattca agtaagagat
     3181 ataattttaa aggcatagta atgtaaaatt tactaaaaaa taagaaattt attttaatat
     3241 ggaggaacaa aaaatggtat cagcaggtga ttttagaaaa ggtgttacat ttgaaaaaga
     3301 tggacaacca tgtttagtag ttgattttca acacgttaag ccaggtaaag gagctgcttt
     3361 cgttagaact aaatacaaaa acttaaaaac aggagctata agagaagaaa gtttcaatcc
     3421 aagtgaaaaa ttccctaaag ctgttataga tacaagacaa atgcaatatc tatacaatga
     3481 tggtgagtta tattatttta tggatcaaga aaattttgaa caaataccat taaactatga
     3541 gcaagttgaa gatgcaatta agttcttaaa agaaaatgaa gttgctacaa taagatttta
     3601 ccaaggacaa ccattccaag tggaagcgcc aaactttgca gagctagaag ttacagatac
     3661 agagccaggt ataaaaggtg atacagcaag taatgtaact aaagcagcta cagttgaaac
     3721 tggagcagtt gttcaagttc cattatttat aaatactgga gataaagtaa agatagatac
     3781 tagaacaggt gaatatttat caagagtata aaaatttatg tatataaaag cttatatgtc
     3841 ttaataatat aagatatatg agcttttagt ttatatataa aaaagaaagc gaggggtgtt
     3901 acatgaaaca tttatatgaa gaagttgcat acttaaaagg cctagcagaa ggactagaaa
     3961 taagtgctga aagtaaagaa gggaaaatga tacataaaat agttgatgca ttagaagtat
     4021 ttgcagatgc tattgtaaca ttggatgaag aacaagaaga acttcaagat tttgttgaat
     4081 ctatagatga agatttagca gatttagaag aagatattgc tgacatggaa gaagatcttt
     4141 atgaagaaga tgacgatgag gatgatgagg acttcagcta tatagaaatg gaatgcccaa
     4201 attgtggaga gttagtagaa atagacgaag atttgttata tgatgatgaa gtagatgttg
     4261 tttgtcctga ttgtaaggct gtgatattat catcagaaga tgattgtgat gatgattgta
     4321 catgtggtgg ttgttcaagt tgtgatgata gagagtaaaa attcataaga attaaaactg
     4381 cctatgatgc ctatgagata ttttgcttca taggtagttt tatttttatg aggaagggta
     4441 ttatgtattt tggttttgca atacttggac aaaaatagta cactagaatt tatagaatag
     4501 gattttggag ggatgtaaat atgaaaataa gtaaaaaatt actagataat atacaaagat
     4561 ttgaaaatgt tataaattat aaatttaaaa ataaagaata tatactagaa gctcttactc
     4621 atagttcata ttctaatgaa aataaaaaat ataattttaa cgaaagattg gaatttttag
     4681 gggattcggt tttaggcata gtgataagtg attatttatt taatgaagaa gctaatctgc
     4741 ccgaagggga actaactaaa cttagagcaa atatagtttg tgaagattca ttaagtgaag
     4801 tagctaatga tataaattta ggtattcaca tgttattagg tagaggagaa gaagcaacag
     4861 gaggaagaca taggacttca atattagcag acgcatttga agctgtaata gctgctatat
     4921 atcttgatgg aggttttgaa agtgcaagac agtttatact tcatcatatg gaaaatataa
     4981 tttatgattc aagaaaagga aatatattta gagactacaa aacgcatctt caagaggtgt
     5041 tacaagggaa tggtgaaaat aatatctggt acaggcttat agaagaaaaa ggaccagacc
     5101 ataataaaag atttgtaatg gaagttggaa ttaatgacga tgtacttggt ataggagaag
     5161 gcaagagtaa aaaagaagct gaacagttag ctgctaaaat agctttaaag aaaaaattat
     5221 gggaaaaata aatataatat gtaaaaatat aaaaagaaat atttatatta gaaggatgtt
     5281 gtagatgaaa aagagaatta tacctatatt tgttcctcac aaaggatgtc ctcatgattg
     5341 catattttgt aatcaaaaaa aaataacagg tgtgagtact gatgttacta gtgaagatgc
     5401 aagaaatata attgaggaat gtttagagac tatagataaa gatgcagatg tagaaatagc
     5461 attttttggt ggtagcttta ctgctataga tattgatata caaaaaagtt tattatcagt
     5521 tgctaaagaa tatgtagaaa aaggtcttgt aaaagatatt agaatgtcta caagaccaga
     5581 ttgtattgat gaagatatat taagtatgtt aaaagaatat aagacaagta taattgaact
     5641 aggagtacaa tccttagatg aaaaagtatt attagatagt ataagaggtc atcaatctga
     5701 aattgtatac aagagttcaa agatgataaa aaatagtgga atcaaacttg gattacagat
     5761 gatgattggt cttccagctg acacagaaga aaagtgtata tttacagcaa aaaaatttgt
     5821 agagctaaaa ccagattgtg ttagagtcta tccaactttg gtagtaaaag atacaggtct
     5881 tgaaaagtta atggaacaaa ataaatataa tccatttact cttgaagaga gtgtaaatat
     5941 agtaaaaaaa gttcttgtac tgttttatgt taataatatc aatgtaataa gagttgggct
     6001 tcaagctact gatgatatac aaattggaaa agctgtttta gcaggaccat atcatcctgc
     6061 atttagagag ttggtagaag cagatatgat aaaagattat ttagaatttg ttattttgca
     6121 aaataaaaat ataaagcaaa tgttggtcaa atctaataaa aagaatattt ctaaaatcat
     6181 aggaaataaa aaaacaaatg ttaaatatat ggaagaaaaa tttggtgtgc ttttaaaaac
     6241 acaagaatca gatttagata taaatcaatt agaaattgtg ttagatggga aaagtttaat
     6301 tagtgctaat atgagagaca ttcatagaaa attgtatgac atttatgacc tttagtttta
     6361 ataatgaagg gagagaaaag atttgtattt aaaaagacta gagttaaagg gatttaaatc
     6421 ctttccagta aaaacagata ttatttttaa agaaggaata acagccatag taggaccaaa
     6481 tgggagtgga aaaagtaata tatctgatgc tgtaagatgg gtattaggag aacagagtat
     6541 taagagtctt agaggtgata aactcgaaga tgtaatattt gcaggtacag atacaaaaaa
     6601 acctatgaat tattgtgaag tggctcttac tattgataat tctgaaaatc aattggaatt
     6661 agattttaca gaagtaacta taagaagaag ggcttataga aatggtgaaa gtgaattttt
     6721 tctaaataat aaaagttgta gattaaaaga tataaaagaa gtctttttag atactggtat
     6781 tggaaaagat ggttattcaa tcatagaaca aggaaaagta gatgaaatat taagtaataa
     6841 tcctttaagc agaagaaaag tttttgatga agcttgtggc atatcaaaat atagatataa
     6901 aaaacaggaa gcagaaagga atttaagtaa tacaaaggaa aatttagaaa gaatagatga
     6961 tgtatatata gaaattgaaa atcaattaaa gcctcttttt aatcaacaga caaaggcaaa
     7021 aaagtacttg gaaataagtg aaaaattaaa gatacttgaa gtaaatagct ttataagaga
     7081 aattgaagga atagaaaaag aattaagtga ggttaatgaa catcgtaagg ttattgaaaa
     7141 ggagctcaat gaaaaagaag aacaaaaaaa tgttgtagag aaaaagcaag aagatattaa
     7201 taaagaggta gaggtattac aagatgtaat tgaaaaatct gtagattata taaattcaat
     7261 taaaggagtt atatcaaaaa aagagtccca aataaattta attaaggaaa gaataagaaa
     7321 ttttacgaat gaaattagta gaaaaaattt agaaataaat gatataaaag agaagctaaa
     7381 tgaaaataag caatacataa aagaattaga aagcaataaa ttatcaggta gtgaagaact
     7441 atctaccttg caggaaaata taaaagtatt ggaatcaagt aaagataaac aaaaaattaa
     7501 gttagaatcc ctaaataatg agatagaatt attgaaagaa agtataattg atatattaaa
     7561 taagaaacaa gagtttagta ataagctgtc cactttaaac gcaaataaag aaaatatgaa
     7621 tatcagagat gaaaatataa atttagagat aacagagtta aacaaaaata ttgaaataaa
     7681 atcatctgaa ttagatacta taaataaaga atttaatatg caaaatgaaa atttaaagaa
     7741 tgtaaataat agacataaag aattatcaat taatttgcag gattcaatta gtgaacacaa
     7801 taaactagaa gatgaaatac agaaaagtaa atataattta aatggatata attcaaagtt
     7861 aaatgtctat atagatatgg aaaatcatta tgaaggattt aatagaggag taaaagaagt
     7921 actaaaaaat aaaaatttaa aaggagttca tggagcatta ggacagatta taaatgtacc
     7981 tgaaaagtat gagaaatcta tagaagcagc tcttggtgct tatatgcaaa atataataac
     8041 tgataatgag tttagtgcta agtctgcaat aaactatcta aaacagaata atttaggtag
     8101 agtaacattt cttccattaa atataattaa atcaaataaa ataagtctag gaaatttaaa
     8161 agcaaatact aagttcatag gaatagcaag tgatttaatt acatttgatg aaaagtatag
     8221 aaatataatt gaaaatatac ttggaagaac aatacttata aataatatag atgagggcat
     8281 aaaatttgca aaagaaactg gtcataggtt taaaatagta actttagatg gtgaaatctt
     8341 aaatccagga ggttctttga ctgggggaag tctaaaaact aatgggaata tactatcgag
     8401 aaaaagatac attaatgaat atacagaaaa gataagtaat atcaagaatg aaatttcaca
     8461 tttagaatta aaaagagaat ctttagacaa agatgtgaaa aatatcaaaa atgaaattga
     8521 ttcacatgaa agtaaaataa aagatttaga aaaaagtata ataataaaaa gtacaagtat
     8581 aaagaatgtt gagtctgaaa ttgaatcgtt aaaaggtagt attactaaat tagaaaatga
     8641 gaagaatgat ttaaattcta acctaaatta tacacttgaa aaaagtgatg atgttagaaa
     8701 agatatggaa gagttagatg atttatacaa taaaaataaa gaaaaaattg atgcattgaa
     8761 tgaggaaata aaaagataca atgatttata tgataaagaa aaatctgaat ttgatgaatt
     8821 gaacttaagt ttagtgaaga aaacagaagt ttacaatagt atagttagag atataaaaag
     8881 aatatctggt gaaaattgtg agctagaaga aaaaaataaa caattagaag agtcattaaa
     8941 ttatgaagaa catgaaataa ttaaattaca agattctata ctcactgaag aaaaagaaaa
     9001 agaaaattta acaaaacaac taggggatag taatagaaat cttgaaacta gaaaaatagc
     9061 aaaagatgat ttaaaaaata gttttgatga aattaataaa gaattaaaaa ctatagatag
     9121 acagcatata gaacttaaag aaagtttatt taaagttggt ggaagattag aaagattaaa
     9181 aactagtcaa gacacatata taaataagtt gtttgagcaa tatgatatga ccttagtaca
     9241 agcattggaa attaaagatg aagatttgga tatagataga aaattcttag aaagtctaaa
     9301 gagagaaata agaagtcttg gaaatataaa tatagactct ataaaggaat atgaggaaat
     9361 aaaagaaaga tacgattttt atagcgaaca aaagcaagat ttagaagagt ctatggaaga
     9421 gatagaaaaa cttatacata ctttagaaga aaatatgaaa tctgaatttg aaattaagtt
     9481 tgaagaaata agtaaaaatt ttaaatatgt atataagaga ttatttggtg gaggttgtgg
     9541 agaattaact attttagata aagaaaattt attagaaagt gatatactaa taacagctca
     9601 acccccaggt aaaaaaatga aaaatttaaa cctattatct ggtggagaga aagctttaac
     9661 ggcaataagt atattatttg ctatattgat tacaaagcca acaccatttt gtatattaga
     9721 tgagattgaa gcgccacttg atgacgcaaa tatatttaga tttggagaat ttttgaagga
     9781 tttatctaaa gagactcaat ttatatcagt aactcatagg agaggaacaa tggaagctgc
     9841 tgattatatc tacggagtta caatgcaaga aaaagccatt tcaaaagtta ttagtttaaa
     9901 attaaaagaa gctcaggaaa taacagatat tatataactt agattgacat aggaggaata
     9961 acagtgttaa aaaaattatt tggttttgga aaagacaagg aaaaggaaat agaaaaaaaa
    10021 gatgctgaag aagaaataga agttgaagac agtgttgata atttagaaaa cttggaagaa
    10081 actatttttt caggacttga agaagaagtg atagataaag tggaagatat tgaagaaaaa
    10141 actgaggaaa gtgtcaataa tgaaggcact gaagaaatcg aaaactttga aaaaatagaa
    10201 gttgagatga atagtaatgg aaaagaagta gaaaatattt caaataatga ttctcaagaa
    10261 gagataatcg aagaattaga aaattatgat gaaattgagt ctaaagagat agaagataaa
    10321 gaagataaga aagttaactt gtttgaaaga ctaaaacaag gattgacaaa agcaaagcaa
    10381 ggaataactg atagaataga tgaagtttta aaatcatata caaagataga tgaagaatta
    10441 cttgaagatt tagaggaaat tttaataaca gcagatgttg gtgtaaatac aactatggat
    10501 ataattgaaa gactaagaga taaaataaag caaaaaggta ttactgaacc aataaaggta
    10561 agagaagaac ttaaatctat agttgaagat attcttacaa atgaaaactc tacactagat
    10621 atagaaccag caccatgtat aatattaatg gtgggtgtaa atggggtcgg taagactact
    10681 acaataggta aactagcaaa tagatacaaa aaagatggta aaaaagtttt attagcagca
    10741 gcagatacat ttagagcagc agcaacagag caattagaaa tttgggcaaa tagaaccaat
    10801 gtagacataa taaaacatca agagggagca gaccctggag ctgtagtatt tgatgctata
    10861 aaagctgcaa aagctagaaa aacagatgta ttaatatgtg atacagcagg aagattacac
    10921 aataaggcta atcttatgaa tgaacttgga aaagtattta aaatagtaga tagagaattt
    10981 ccagaagcaa aacgtgaagt acttcttgtg gtggatgcta caacaggtca aaatgcagtt
    11041 gttcaagcaa agacatttaa agaagttgca gatataacag gtatagtact tacaaaactt
    11101 gatggaactg caaaaggtgg agtagtcctt gcagtaaaat cagaagtaga tgtacctgta
    11161 aaacttattg gagttggaga aagtgtagaa gacctacaag atttcaatgc aaaatcattt
    11221 tcagatgcat tatttggtaa ctaatgttct tttaaaaata atggttgaca agagtgttgt
    11281 tatgatataa aatacaatag tgtaaagtaa ataaccttta cagtatactg ctaggaagtg
    11341 taagatatga atatagaaaa aatggtcgaa attggattgt tatttgaaca gtataaagaa
    11401 ttattaactg acaagcagaa agaaatagtt gctctatact atgaagaaga ttattcttta
    11461 ggtgaaatta gtgaaaatct gaatgtatca agacaaggtg tttatgatac cttaaagcgt
    11521 tcagaaaaaa tactaagaga ttatgaagaa aagttacact tagtatcaaa gattcaagag
    11581 caggaaaaaa atattaagat tataaaagat aaaattattg acattaaaga agatttattg
    11641 cacaatagag attgtgctaa tttaatccct aagctagaaa atatagaaga tgtatgtagg
    11701 gagatgataa aatgatattt gaaggattgt ctgataaact acaaggggca tttagtaagt
    11761 taaaatctaa aggaaaactt acagaagtag atgtaaaaaa tgctatgaga gaagttaaac
    11821 ttgctcttct tgaggcagat gttaacttta aagtagttaa agatttcata aaaaaagttc
    11881 aagagagatg tgttgggcaa gaagttatgc aaagcttaac tccagctcag catgtaatca
    11941 aaattgtaaa tgaagagctt acaagtttaa tgggagatgt acaaagtaaa gtaatgattt
    12001 cctcaaagcc tcctacaatt ttaatgatgg ttggattgca aggtgcaggt aagactacta
    12061 cagcaggaaa acttggagga tattttaaaa agcaaggtaa aaaacctcta ttagtagctt
    12121 gtgatattta tagaccagca gctataaagc aacttcaagt tgttggagaa aaattggata
    12181 tacctgtatt caatatggga gataaagaaa atccagtaaa tattgcgaaa gcaggtttaa
    12241 gccatgcgat taaaaatgct aatgatttag ttattataga tacagctggt agacttcata
    12301 tagatgaagt acttatggat gagttaaagt ctattaaatc agaagttaag ccacatgaga
    12361 tacttttggt tgtagattct atgacaggtc aagatgctgt aaatgttgca gaaagcttca
    12421 atgaggcact tggtgttgat ggagtagtat taactaaatt ggatggagat actagaggtg
    12481 gtgctgcact ttcaataaga gcagttacac aaaagcctat aaaatttatg gggatgggag
    12541 aaaagctaga tgatatagaa cctttccacc cagatagaat ggcatcaaga atattaggta
    12601 tgggagatgt cttaagttta atagaaaaag cacaagaaaa tatagactta gagaaagcta
    12661 aagaattaga gcaaaaaatt aaaaaacaag agcttgattt tgaagatttc ttacaacaaa
    12721 tggaacaaat acaaaatatg ggacctcttg acaagatact tggtatgata cctggtatgg
    12781 gaaatgtaaa agaccaactt ggagatgtag atttgaatgg caaagaaatg aagagaacaa
    12841 aagccataat tcagtcaatg actacatatg agagaagaaa cccaggagta ttaaatgctt
    12901 caagaaagaa aagaatagct aaaggcagtg gaactagtgt acaagatgta aatagactta
    12961 taaaacaact taatgaaatg aaaaaaatga tgaagatgtt tgctggttca caaaagagta
    13021 tgaagaaaag gggcggctta ggcggtttac ctttttttaa ataaaatata aaaaagtaaa
    13081 aattattcgg aggtgactgt aatggcagtt aaaataagat taaaaagaat gggagctaac
    13141 aaaaaacctt tctacagaat agtagtagct gattcaagag ctccaagaga tggaaaattt
    13201 atagaagaaa taggatacta taatccaatt tctgaaccta aacaagtaag aataaatgat
    13261 gaaaaagcta taaaatggtt agctactggt gctcagccaa cagaagtagt taaaaaatta
    13321 cttgtaaaaa atggagtaat agaaaaattc gaagcttcta aacaagcaaa ataatttttt
    13381 cttggcaatt agtttaagta agggctcatt gctaaggtta ctttaggaga tgaaatatat
    13441 gaaagagcta gttgtagata tagctaaggc tctagttgat aatcctgact cagttgttgt
    13501 tgaggaattt gaagataatg atggtatcat cttaaagctt acagttgctc aagaggatat
    13561 gggtaaggtt attgggaagc agggaagaat agctaaagct ataagaactg tcgtaagatc
    13621 tgttgcaaat agagaaaata taaaagtttc tcttgagata gtataaaata ggaggattag
    13681 gtataatgcc tagtcctttt tatataaatg cgtagtttaa gattttatga tgatatgacc
    13741 acataagatt aaatttttta agaaggtgat aacatgaaag aaaaattaac tcattttaag
    13801 gtaggtcaaa tagtaaatac acaaggttta aaaggtgaag taagagttta tccacttact
    13861 gatgatatag agaggtttga tgaattaaaa gatttctatc tgggaaaaga tttaaataat
    13921 aaattggctg ttgaaaaagt aagatacaaa ggttctatgg ttataatgaa gataaaaggc
    13981 atagacagca tagagaaagc agaaaaacta aaaaataaat ttatgtatgt atctagagaa
    14041 gatagcagag agcttgaaga aggcgaattt tttatagcag atatgattgg aatggaagtt
    14101 ttaactgtag atggaaaata tgtagggata ctagaagatg ttcttcaata ctcagcaaat
    14161 gatgtctatg tagtaaaagg tgaggaagat aaagaattta tgataccagc tataaaaaaa
    14221 tttgtaccta caatagatat tgatgaaaga aaaatgatta tagaccctat taagggaatg
    14281 atagattagg tggtaatatg agatttcata taatgacact ttttccagaa atatttaatt
    14341 cgtacatgga tgaaagtata atgaagagag cagttgaaaa aggtataatt gaggttcata
    14401 tatataatat aagggatttt tctaataata aacataaaaa agtagatgat tatccatttg
    14461 gaggaggagc ggggatggta atgactcctc aacctatata tgacacatat aagcatataa
    14521 taacaactca taatataaat aacccaagtg taatttatct tactccaaaa ggtaaggtat
    14581 acaatcaatc tatggcaaag cagatgtctt taaaagaaga tataatacta ctatgtggtc
    14641 attatgaagg aatagatgag agaatcattg atttaatagt tactgatgaa atttcaattg
    14701 gagattatgt attaactggg ggagagctcc cagcacttat aatgatagat tctatatcga
    14761 gattaatacc aggtgtatta aaccaagaag aatcatttga agaagaatca tttaaagaca
    14821 acttattgga atatcctcat tacactagac ctagagattt tgaaggatta aaagtaccag
    14881 aagtgctttt atcagggaat cataagaaga tagatgagtg gagaagagaa gaatctataa
    14941 ggataaccaa agaaagacga tttgatttat ataaaaagtc taatgaaaaa taaaaatata
    15001 atgaaaaatt aatatatatg tatacaataa agtacctata aatggaatta aaaacataaa
    15061 agtgcttgta ataactattg aagtatagta taatataaaa tgttgaaaat acgggcgttc
    15121 ctctgtttac actggtcggg tagttaagaa cgtctatgat atctaggagg aagaaagatg
    15181 aatgaaatgc taagagcaat tgagcaagaa caattaaaaa atgaagttcc taattttgga
    15241 cctggggaca cagttaaagt acacgttaga attatagaag gaaaaagaga aagaatacaa
    15301 gtattcgaag gtgtagtatt aaagagacaa ggtggaggag caagagaaac tttcacagtt
    15361 agaaaaatgt ctttcaatgt tggtgtagaa agaactttcc cagttcactc tccaaaaata
    15421 gaaaaaatag aagttactag aaaaggtaaa gtaagaagag ctaaactaaa ctacttaaga
    15481 ggtagagtag gtaaagctgc taagataaaa gaagctagaa ataaataatt aaaaacttta
    15541 cttataaatg gggggctgat ttatcagtcc tctttctatt ttaatgaaaa ttacagcaaa
    15601 taattatact agaaataaat agaaatattt gttgaaaata tacattattt atctatgaat
    15661 ttatagcttt taaaatgaaa aataaaattg gtaaatattg cattatattt tgtaacaata
    15721 ttaaaaataa gagaacagta ttatgtaaag tatgacgtta aaaagatgac aatattatag
    15781 tttgttaaga tagatttttt aagtggaatt aggatataat aatataatat taataaggag
    15841 tgatacatgt gtcaaatgaa tacgaagagt atcttatgaa tgacaatcta catataaact
    15901 ggtatccagg tcatatgaaa aaaactaagg agttagtaaa aaataactta aaattgattg
    15961 atgtagtaat tgaacttttg gatgctagaa tcccgtttag tagtaaaaat ccagacatag
    16021 acaggcttgt aggagataag ccaagagttg tcgtacttaa taagagtgat atggcagata
    16081 gggataaatt gaatcaatgg attgaatact ataagaaaat taatatcaaa gctataccag
    16141 tagatacaat aaaaggcgtt ggtataaata agataatcga agaatgtaaa aatgtaacta
    16201 gagaaaaaat gagttcttta aaagacaaag ggagaaaaga aagagctata cgaataatga
    16261 tagttggagt tccaaacgtt ggtaaatcgt ctcttataaa caaattaaca ggtagaaaaa
    16321 gcactcaaac tggagataag ccaggagtaa cgaaagggaa acaatgggtt agattaaagg
    16381 gaaatcttga acttttagat acacctggaa tactttggcc taagtttgaa gaccaagaag
    16441 ttgcattaaa tttagcattt agtagagcta taaaggatga gattttagat actgaaactt
    16501 tggctttaag acttatagag aaacttatga aaatagaacc tgagaagttg aaagccagat
    16561 ataagttaga ttgtctagga gaaactccaa ttgaaactat ggatatgatt ggccataaaa
    16621 gagggtttat aacaggtaaa aaagaactag attatacacg catagccaca actgtattaa
    16681 atgaatttag agatggaaaa ataggaaata taacattgga agttccagaa aacgttaaaa
    16741 gatagatttt aaaacaaaat caaaaattta atgttaaaaa tttaaaaaag ccttttacca
    16801 aaggcttttt tatttttttg aaatatagac aaatatctcc atataaaagt ataaattagg
    16861 ttagaaaacc tttaaaaata ttaatttaag acaaatatat ctaatatttt gaaaattaaa
    16921 atgagtgtga ttatttaaaa ttttagcata ttgacttgct tgtttcgtat aatatataat
    16981 aagcacaaat aaatacaata tattaaaaat ttaatttatt taattttatt aatttaataa
    17041 taagttctta ttgtttaatt caacttactt atgcaagcgt aaacaatcga gacattttat
    17101 taatttaata aggttaaaaa gggggatttt ataatgggta agaccaagga tgttatagta
    17161 tttggatttg cgttatttgc aatgttcttt ggagcaggta acttaatatt tccaccttac
    17221 ttgggaataa ttacaggtcc agaatggtta attgccttct taggatttac gtttgctgat
    17281 gctggattgg cacttctagc agtaatggct acagctaaat tcgatggaaa cgtagttgaa
    17341 atgtttaaaa gatgtggtat gaagttaggg attcttatag gttgtgcaga tattttatgt
    17401 ataggcccat tcttagctat accaagaaca ggtgcaacaa cttatgaaat gggtataatg
    17461 cctttatttg gaacttcaat tccagtgtta ttattctgta tacttttttt tgcgatttca
    17521 tatgtactaa caataagacc ttctaaggtc gtagatatag ttggacagtt tttaactcca
    17581 gctcttctaa tagctttagc atttataatt ataaaaggta ttatatcacc tcttggagat
    17641 atagtagata aacctatgat acctaatgta tttgcagaag gtataggtca aggatatcag
    17701 acaatggatg cttttgcagc aatagcactt gcatcagtgc taatagtatc tttaaatgat
    17761 aaaggatatt ctactattag tgacaagttg aaaatgattg gtaaggcggg tgttcttgct
    17821 tgtggaggat tagctctagt ttatggagga ttatgtttcc ttggggcaac tgtatcaaca
    17881 atgtatggaa cagatgcagt acaatcacaa gtaatagtaa atataactga aggattgctt
    17941 ggaaatgttg ggaaagcgat tttagcagta gtagtatcat tagcatgttt aactacatct
    18001 ataggtctta catctgccac aggtcaatat ttctctagac ttacaaaagg taaattgagt
    18061 tatgaaaaaa tagtattagc tgtatctgta ttcagtgcag tagtagcatc ttttggtgtt
    18121 ggaactataa taaagatagc atctcctata ctaagtatag tttatccacc atcaattgtt
    18181 ttaataattt tagctttctt caatgaaaaa attaagaatg ataatgtata caaaggagca
    18241 gtatatatgt cattgcttgt aagcatacta acagttatat ctagttatgg agtagctgtt
    18301 ccagtagtaa attctttacc tcttaattca ttaggattta actgggtggt gccagttata
    18361 atagctggaa taattggtaa ttttatacca tcaaagagtc aatctaatac acttggaaca
    18421 aactaagttt tgtttaatta aaatataatc aattgtaaat aaatgagtta actaaaagta
    18481 aaaggagtta tttgtacaa
//
LOCUS       DAEMHW010000068        18128 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEMHW010000068 DAEMHW010000000
VERSION     DAEMHW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18128
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.068"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(97..1818)
                     /locus_tag="KPK67_001939"
     CDS             complement(97..1818)
                     /locus_tag="KPK67_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HBG2458908.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGNTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGPTGADGEVGPTGAVGATGPDGLVGPTGPTGATGANGLVGPTGPTGATGANGLVGP
                     TGPTGATGANGLVGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGAN
                     GATGANGATGPTGAVGATGANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTG
                     AVGATGANGVAGAIGPTGPTGANGATGATGATGANGATGPTGATGATGVLATNNAQFT
                     VSSSSLVNNTLVTFNSSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSI
                     TTNFNGVAQNNFIAKAVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGY
                     VSVYRIQ"
     gene            complement(2196..2735)
                     /gene="hpt"
                     /locus_tag="KPK67_001940"
     CDS             complement(2196..2735)
                     /gene="hpt"
                     /locus_tag="KPK67_001940"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG2458909.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(3063..4370)
                     /locus_tag="KPK67_001941"
     CDS             complement(3063..4370)
                     /locus_tag="KPK67_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBG2458910.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4795..6570)
                     /locus_tag="KPK67_001942"
     CDS             complement(4795..6570)
                     /locus_tag="KPK67_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2458911.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIKNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6567..6704)
                     /locus_tag="KPK67_001943"
     CDS             complement(6567..6704)
                     /locus_tag="KPK67_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458912.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6782..7351)
                     /locus_tag="KPK67_001944"
     CDS             complement(6782..7351)
                     /locus_tag="KPK67_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2458913.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7535..8170)
                     /locus_tag="KPK67_001945"
     CDS             complement(7535..8170)
                     /locus_tag="KPK67_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG2458914.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            complement(8838..9719)
                     /locus_tag="KPK67_001946"
     CDS             complement(8838..9719)
                     /locus_tag="KPK67_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG2458915.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            complement(9839..10846)
                     /locus_tag="KPK67_001947"
     CDS             complement(9839..10846)
                     /locus_tag="KPK67_001947"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBG2458916.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            complement(10866..11348)
                     /locus_tag="KPK67_001948"
     CDS             complement(10866..11348)
                     /locus_tag="KPK67_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG2458917.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            complement(11366..11833)
                     /locus_tag="KPK67_001949"
     CDS             complement(11366..11833)
                     /locus_tag="KPK67_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG2458918.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            complement(11848..12606)
                     /gene="prdD"
                     /locus_tag="KPK67_001950"
     CDS             complement(11848..12606)
                     /gene="prdD"
                     /locus_tag="KPK67_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBG2458919.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMIFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            complement(12686..13411)
                     /gene="prdB"
                     /locus_tag="KPK67_001951"
     CDS             complement(12686..13411)
                     /gene="prdB"
                     /locus_tag="KPK67_001951"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(12959..12961),aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBG2458920.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            complement(13449..13733)
                     /locus_tag="KPK67_001952"
     CDS             complement(13449..13733)
                     /locus_tag="KPK67_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458921.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            complement(13814..15694)
                     /gene="prdA"
                     /locus_tag="KPK67_001953"
     CDS             complement(13814..15694)
                     /gene="prdA"
                     /locus_tag="KPK67_001953"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBG2458922.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            complement(16123..17883)
                     /gene="prdR"
                     /locus_tag="KPK67_001954"
     CDS             complement(16123..17883)
                     /gene="prdR"
                     /locus_tag="KPK67_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBG2458923.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
BASE COUNT         5204 a         3587 c         2134 g         7203 t
ORIGIN      
        1 tagtcttttc atcatataaa cctattaaat attattctat ttattggtat atgaaatcat
       61 attgtttatt attttataat cttttcatca tataaactat tgtattctat aaactgatac
      121 atatccagct gcagaagtac ctgtcgtgcc tgaaccaaat gtaaaactta aagtaacagc
      181 tgctgctcta gcatcaacta aaaagcttaa acttacactt acatctgatg aagtaagtgt
      241 atttactgct tttgcaataa agttattttg agctactcca ttaaagttag tagtaattga
      301 cataaatcct gcaagtgaaa gtgtggcacg tataccgaaa gatacattgt atatccctcc
      361 aactgcaaga tttatagtac tacttgttgg aaaagttata ttagttccat ttataaatga
      421 tgaattaaat gtcactaatg tattatttac taaacttgaa gaggacactg taaattgtgc
      481 attgtttgtt gctaacactc ctgttgctcc tgtcgcccct gttggacctg ttgctccatt
      541 tgctcctgtt gctcctgtcg cccctgtcgc tccatttgct ccggttgggc ctgttggtcc
      601 tatcgctcct gctactccat ttgctcccgt tgctcctact gctccggttg ggcctgttgc
      661 tccatttgcc cctgttgctc ctgtcgctcc tgttgctcct gctactccat ttgctccggt
      721 tggacctgtt ggacctattg gacctgctac tccattcgct cccgttgctc ctactgctcc
      781 cgttgggcct gttgctccat ttgcccctgt tgctccattt gcccctgttg ctcctgtcgc
      841 tcctgttgga cctactgctc catctgctcc cgttgggcct gttgctccta ctgctccggt
      901 tggacctatt gccccagcta ctcctgttgc tcctgtcgct cctgttggac ctaccaaacc
      961 atttgctccg gttgctccgg ttgggcctgt tgggcctacc aaaccatttg ctccggttgc
     1021 tccagttggg cctgttgggc ctaccaaacc atttgctccg gttgctccag ttgggcctgt
     1081 tgggcctacc aaaccatctg gacctgttgc tcctactgct cctgttggac ctacctctcc
     1141 atctgctccg gttggacctg ttgctcctat tgggcctgtt gccccagttg ttccggttgc
     1201 tccagtcgct cctatacttc cggttggacc tgttgctcct gtatttcctg ttggtcctgt
     1261 agcacctgtt actccatttg ctccggttgc tcctgtattt cctgttggac ctgttattcc
     1321 atttgctccg gttgctccgg ttgctcctgt atttcctgtt ggacctgttg ctccagttgc
     1381 tcctgtattc cctgttggtc ctgttattcc atttgctcca gtcgctcctg tatttcctgt
     1441 tggtcctgta gagcctgtta ttccatttgc tccggttgct cctttgtttc ctgttggtcc
     1501 tgttattcca tttgctccgg ttgctcctgt attccctgtc ggacctgtta ttccattcgc
     1561 tccagtcgct cctgtatttc ctgttggtcc tgtagaacct gttgttccat ttggtccagt
     1621 tgctcccata tttcctgttg gacccgttat tccattcgct ccagttgctc ccgtatttcc
     1681 tgttggtcct gttattccat ttgctccggt cgctccggtt gcacctctag gccctgttgg
     1741 tcctgttgga ccagtagcac ctgttggacc tgttggacct acatcttgat ataaacttgg
     1801 acctgaaata tcactcatat aattaatcct ccttttttaa agttagagta ttactctata
     1861 aatattgagt attactctat ataattatat gaatcgacaa tttcttatgt gataatgtat
     1921 ctatttgctt atatcaacat atatgctttg attcttaaat ttcattactt ttttgtaaaa
     1981 tcttttacaa ttattagtac tcttttacaa ttattaatac aattaaaaaa ctcatttccc
     2041 atcaattatt taaaattttt ttattatgtt ataaaaaata gataattaaa ttgcataaaa
     2101 ttaatttaat actactttaa ttacttttta aataaaaaaa cctcaacacg attaataaaa
     2161 atcatattaa ggcatccaca aaattatata attgcttatc tatcttcatc agtaacattg
     2221 aatatataag gaacatttct ataataatca ccaaaatcaa acccatatcc aaccacaaac
     2281 ttatcttcta tttcaaatcc acaataatct ggcactaaat cgacttttct tctgcttggt
     2341 ttatccaaaa gaacacagca ttttaaacta gcaggatttt tagcttttag atgttttaac
     2401 acaaaatcca tagtaagtgc agaatcagta atatcgtcta caaccaaaac atcatatcct
     2461 gctatatcag tagtcacatc tgaaactact ttaacctttc cagtactttc ttcattgttt
     2521 ccataactag atgtagtcat aaaattaact ctaagtggta agtttatatt tctaactaag
     2581 tctgcacaaa atataaagct accttttaat agagaaacta ccataagatt tttatcttta
     2641 taatcttttt ctataattgc tcctaattcc cttaatttat ctttaatttt ttcttctgaa
     2701 tacaatactt cccatttttt tgtttctata tccataaaaa gacctccaat tgtaataatc
     2761 cttcttaatt ttataagaga ttgctcactt tttcaatact cttattcaat attagtcaat
     2821 attattgagt ttttatgttc attcaacaat tattaacaat gtatcttata ttttaagtag
     2881 ttcatatatc cacactaaat tacatactat atattttaga caaaaaaaga aaaaacttgc
     2941 actaacaagt tttttctatt atacataaat ttatacataa tcatatttta ttagagggta
     3001 ctaattaaat ttatttatgc tatttttgtt atacctcata atacaaaaca tattataaag
     3061 tactagtcaa ctttattcat atcattcatt acatttaaga ttatctcatc tgttatagac
     3121 atacctttgt cacttactct acctaagttt tgtatacttt gctcaacact tgaaccaact
     3181 ataccattta gtggtggtac aaaacaatct tgcttagcta ttatagcact ttgtatagct
     3241 gcagatgctg cagaagataa ttttaatgca caaccagctt tcgcaccatc acatatcatt
     3301 ccacttaagt cagctatcat attttctata gctccttcta tttgcttttc agtaccattc
     3361 ataagccaag agataccagc agttgctcca gtagaagcag caacaccaca tccacataca
     3421 gcagataatc ttcctgtata gttcttaata taaccagtta ctagatgaga tatagctaaa
     3481 gcttttgcta gtctttcatc agattgtggg aattttttat tataagcaac gattggaaga
     3541 attgctgtta atccatgatt tccacttcca tttgaactca taacaggcat ttttacccct
     3601 gccattcttg catccgaagc tccagcagtt aacatcattg cataatttat aacatcatta
     3661 cctaaaagac cttcttctat agacttttta attccatatc cgacaccaat accagttttt
     3721 tgttttaatc catattcagc catctcttca ttcattttta ctccatctag taaaaattct
     3781 atatctttaa aatcaatttc ttctacattt tttattaatt cttgtatagt tactgtatcc
     3841 attaaacttt cagctttttc tgccgcatca tttgaagctg atacttttgg ctcattgtct
     3901 aataatactt ctccatcttt ttctaagaac gtaaaattat catgtttagt tcttattctc
     3961 actttagcaa tatgattttt tccttttaac acaacttcta taaatacttt ctctcttgta
     4021 tcagcaggtg ttattgatag tggagtatta tccatataat cctcagctat tttaacttcc
     4081 tcttttgtta gagtttctaa cacacttaat ccattttcag aatgtccacc tactatacct
     4141 agagctgctg caatttttaa tcctaatctt tcagtacctg gtatacctac gcacatacca
     4201 tttttataga tactaggact aactaacata cgattttcaa ctatttcttc tcctaataat
     4261 tcctttgcct ttgcacatgc tagagctaag gctactggtt cagtacaccc tactgctggt
     4321 ttaacttctg cctttaacat ttccactaat tcttttctta tatctctcat tatatataaa
     4381 cctcccaatt aagtttagat ttagtactca aataattaat taaataattt cttatctata
     4441 tttacagcaa aatacatgcc atatttttta tatcttttta tatttttagt cttatattct
     4501 ataaccattg gtatgactgc ttctatttat gtattttttt tatttacata gagtatttat
     4561 attctttaaa atggagaaac atatgacaat ttcgcaaaaa gacaaaaaaa agtctcaaaa
     4621 tgagattcta tatcattttg agacggctca aatgtattat tttttaactt ataaaaacca
     4681 ttttttaatt cataaaaaaa gattctgcaa aacacttata gtataagttt aaagctttct
     4741 ttaaagttat gaatttagaa aaatttaaat tttatatagc tttttattat attactatga
     4801 aattatattg tatttttcaa tttttctata taatgtagct cttgatattc caagtgcttt
     4861 agctgttaat tctttatctt ttttataatc tttatactta tttaaagctt ttattatctc
     4921 aatcttctct agttcatcaa gtggaattat cctatcgctt ttaacttcta actcttctac
     4981 agaggtattc ttaatttttt gaggaattac attcattgtg attattgaag aagaagacat
     5041 attcacacta tattcaataa tattctgcaa ctctcttata ttccctggcc acttatattc
     5101 catcatagca tcaacaaccc tgctatctat gtcaataaca tttttatcta atttttttga
     5161 atactcttta accatatgct tgattataag tgggatatct tcttttctct ctcttaaact
     5221 tggtaatttt ataggtataa catttaatct atagtataaa tcttctctaa aagtgccatt
     5281 ttgaaccatt ttttctaaat ctttatttgt agcagcaata attcttacat ctattgaaac
     5341 atttgattta cttccaattt tattaagttc cttttcttgt aatactctca ataactttga
     5401 ctgtaaatgc aaactcatat ctcctatttc atctaaaaat atacttccct tatgagctac
     5461 ttcaaacatt ccaagctttc cacctttttt agctccagta aaagctccct cttcatatcc
     5521 aaataattca ctctctaata aattgtctgg tatagcagca cagtttacag ctataaaagg
     5581 attatctact ctattgctat ggttgtgtat ggctcttgca aatagctcct tacccgttcc
     5641 actttcccca gtaataagta ctgttgatgt tgatttagaa gctattaggg cttccttttt
     5701 agaatttttt atagctacac tttctccaat tatgttttcc aattttattc tataatcttt
     5761 attcatctta ttgtagtttt ttatagcatc tcttttatct ataaaatcaa taacatatcc
     5821 ctttaactca ttttttaata ctattttatt gatgttgtat attcccttcg aatcatatcc
     5881 gtatctttta taaaaaaaac tacaggattt gtccttctta aagttattta tagaaggttt
     5941 ctttataaaa tctaattcga caaatatgtt tttgccagtt atactatctt ttatattgaa
     6001 aagctcttta aattttgaat tatatttatc aatttttcca tccacatcaa tagacacaac
     6061 agctttgtcc atattgttta atagtgtctc taattttttc ttttctaatt caagctcata
     6121 agttttaatt tgtgctttta atttattaga tattaggtct gccattttgc ctaaaaagtt
     6181 tataagacca cttttattat tttctataat ttgtgcttgt tcttttgtaa aggctataag
     6241 gcctataaca ccatgagaat cgccatcaca tataatagga caacacacct ctgcaaactc
     6301 tacacaagcc ccacttttat agcattccct acatatatca ctactctttg ggtcatcaat
     6361 tagtattgta atctgttctg taagtgattt tttaaaagcc gtatatttat ctactttctc
     6421 acctattttg tctatatata ctcctgtacc tgcaatccta aataactcct tatttactat
     6481 agttacatct atattgagta ctgatactat agcttcagct atattttgaa tatcagattt
     6541 tatatattct aaacctatca taatcatcat tccttatttt tctttaacat aagtatttgt
     6601 cttatttctt taagaatcaa gtatatctta tttaaagtaa attggattga catcaggatt
     6661 acaaagagca taacaattat tattaactca taatttattt ccatctaaac atctccttat
     6721 aacaaaagac taggcttaac ctagtctttt atattatatc catatttata ttttaactca
     6781 tttatcttct ttttcccaca taaatatatt ttaatttatt tgactcttta atcataccat
     6841 caaagtttaa tcttgaaaat atattaaata cttcatgtgg atagtgaatc tttgcaacag
     6901 aaccacaaaa tttatgttta agatagttta acttaacttt taaattcttt aaatttaaat
     6961 cctctaatat aacctctcca ttgcccttta acactctttg tatttcagat gaagcttttt
     7021 cacaatctgt aataacatta aattggtcaa ctattattat cttgttgaac tgattatcat
     7081 caaatggtag aaactctaac ttacctttaa ctacattgca attttcatta tacactagag
     7141 aataacagac atcttcataa ttactattct ccagtatagt tacagaagtt ccaagacctc
     7201 tcaatctctt gcctaggttt cctatatgac caacaagcaa tattttatca cctacattat
     7261 actcaattgt atccaaaaaa cattctaatt catcagatgt atatttagct ttaaccggct
     7321 gtttatttat ctgacataat atgcttttca caatgcttca cctcttcatt tcctcaatta
     7381 taaattatgt acagattaca ttataataaa tggcgagcaa ctattcaaga gatatttcat
     7441 tagtattttt ttaaacaatc tttatacatt ttaatatgat attataccaa atttattagg
     7501 attttattca taaaaatcag tctatttttt atgtttattc attgttgttt tcatccaatt
     7561 tttttatttt cgatatggat acttcatatg ccattttttt gaccacatta ttttcatcta
     7621 tttttttctc atattctcta ctttgaaccc taccccaaag ctgtattcta tcaccaacct
     7681 ttagattctt tgcaaattta gcatttctac cccaaactat tgatggtata taatcagatt
     7741 tattataagg tctgtttata gcaactaata aatcggttat ttcccttcct aaaggtgtct
     7801 tcctatacac aggttcctta catacatatc catctaaaaa tatactatta gggtctttat
     7861 tttcctcgtc tatgcttttt atttctctga caaacactgt aagcactaat ctatttttat
     7921 tatttaatgt tttgttatat gatcttaatt ggcctacgac attgactaaa ttttgtttat
     7981 caaggtcaat atcttggaga agtctttctg atacagtcat tggaagtata tcaaaagaat
     8041 cgcttagtct gtttattttt attttcatat tataaaattt ttctccaaat atctcatggc
     8101 taaattctag cttattgtct aactcccctc ttaagtttac tacgttattc tcatctttaa
     8161 cagctatcat attttatccc tccacaattg ttaattctta tatttctggt atcagcttat
     8221 ataataaata tatttataag ggatgtattt tattacaaat ctttgattgc tgtttaaaag
     8281 tttttattta gttataaatt tcatttaata tttcactttt ataattataa aattattatc
     8341 tttttgtacc acatatacta attaaactac ctatttcaat tttgtattaa ttataaaaat
     8401 cctattatat agttaataag tcatatataa cttaaattta ttcactctat ataagcaact
     8461 tcaacgaatt aaatttattc aaataattca gatgaatatt gagtcattat aaacttattt
     8521 ttcttaatta taagttatat tagacttatt acagcttata tttttattaa tctgtattaa
     8581 tatatgaaca tttattgaac ttattgatta taaaatttaa atatttagct ttaaattatg
     8641 ctttattctt taaaaataca acgtttttta cattaaatct actaaatcac tatatatatt
     8701 acataattgg catgttaatt gctttatatt aatttagtaa ataaaaaact gccctaaaat
     8761 attaattcaa tattttaaag cagtttataa tctttattat tagctgtaat atttaataaa
     8821 cactaagtag tctttaacta agcagtcttt ttgtttttat ttgcagccat tatcattgta
     8881 gcagaagtat aagctataac tactattact aatgctttta atatatccat tggtagagtt
     8941 tttactatat atgcagctac gaaaactcct attaaaccac atatagctat tataactgat
     9001 gcttttttag tatatgcatc ttcttttatg aatttcatac ttgcaactgg cattaataat
     9061 gcacaagaac ccataactat tgggaatgca acttttgcag acattcctaa gaaataaacc
     9121 attgccatac ctggagcata tagacctata ccagctgtca ttaaagctcc tagtataaag
     9181 ttacctatta cacctattac taatttagca ccagtaagac ctaattcatt tcctccacct
     9241 ggtaatacgt taatccaagg atgacctaat aacattaata ttgctgttaa tgctaaagtt
     9301 acacccataa caagttgtat ttttctctcg tccatcttag ctattattcc tgcacctata
     9361 tatgaaccaa ctgctgctgc cacaaccata gatactaaag taacaccatc aactggtatg
     9421 attgatgtaa atatgaatgc ttctgtaaca actggaatag tatgtgctac gtttaatgta
     9481 ccaggcattt ttttatctgg tatatttaac tttaaagcct ttgacattgc tattgttgga
     9541 gcaaatgacc ctattcctaa tgtatcaaag aagtctgtta taaatcctgt tacagcaact
     9601 tttggagtac tttgttcttc tagtttcccc tctttgtttg ctttagctac gtctttacca
     9661 aacacaactg caaagattgc tgcgaatact acatataatg cctttaagat tgctaccata
     9721 atccatcctc ctattttttt attttttcat atattctgaa tcttatagcc taaaagctat
     9781 agaatagcaa ccgacaagtg gctactattc tattttaatt tcctaattta tttattactt
     9841 atttaagaat aaatccatgt ttaagtgggt cttcttcatc tattacaaaa tgattaaatc
     9901 cagttatata agcagaacca gttattttag gtactacagc attgaaatct gcaactttag
     9961 tttcttcaac tatttcacct ttgaataaag ttcctaatat actttcatat acgaattttt
    10021 ctcctacttt taattcacct ttagcatgta aagtagctaa tttagcactt gttccagttc
    10081 cacatggaga tctgtcaact tgaccttgac caaatataac tacatttttg taagtagctt
    10141 ctggatgagt tggctcatca tatatttcaa ctaagtctac agtttttata tgagctaaag
    10201 ttggatgttg tatttctatt ttctcattta ttatatctct aagtttcata gctagttcag
    10261 ttaatttacc agcattttga ggttcgattt ttaaacctaa ttgacttgca tgtattatag
    10321 caaagaagct tccaccaaat gatatgtcaa atttaacagt tccaacacct ggtaagtcaa
    10381 cttcaacacc ttctttatat aagaaagctg gtacgtttaa gaatgatact tctttagctt
    10441 ttccatctac aactgtaacg tctcctctta ttattcctgc tggagcttcc ataactacat
    10501 gagttacagg ctctactgct ggaactacac ctgtttctat agctgctgtc atagctccta
    10561 ttgtaccatg gccacacatg ttaaggtatc cgccaccatc catgaagatt attccaaagt
    10621 cagcatctgg gcaacaaggt tgagtcatta cagaaccaaa catatcatta tgtcctcttg
    10681 gctctaacat tatagcagtt cttaaataat ctagattttc ctctaaatat tcttttttct
    10741 caggcataga atttcctttt atgttaggta ttccacctac aactattcta gttgcttcac
    10801 ctgctgtatg agagtctata gcttgtatac ttctgctaaa tttcatatca aattccccct
    10861 taggtttatt ttgcgtcttt acgcattttt aattttagga aagccaatgc ttttttaaca
    10921 tcaacttcat ccatgtgatt gtctccaact acttctgttt ctatgtagcc tgttgtcttt
    10981 ttataatcaa ttatggtagc tttatccaaa tatggattgg taattactaa tccatctgct
    11041 cccataatag taagacctac tgtaggtata tctgatttta caaactctgc tatagcattt
    11101 gtaaaatcaa tgtgctggtt tcctgccata tctaaagtta taatcactcc atcaggtcgc
    11161 aatgattcaa tccatcttcc aagtctagtt gctaaaaatg ttttccattc attgtgctct
    11221 gatgtcccaa ctacaaccac tcctaacaag tctatgtcag aatcattatc tatcaaatct
    11281 agtaacgggt ctctaaatat atgaagtgat gtgtttttca ttgatgtaga aattcccaca
    11341 aaactcattc ccttacttaa attttttatc catgttttct cattttaagt tttaaaaatg
    11401 ccaaagcttt tttagcatct atttcatttg tgttgttttc tccaactact tctgtctcaa
    11461 taccttcttt tgatttgttc atatcaacta tggtatccat atattgattg cttacaacaa
    11521 acttagcttg agtcccatta aatgtaactc caactacagg tatatctctt tttcctattt
    11581 cctcaaatgt gttagcgtag tcaacatgtg agtttcccca accatctgat gaaactatag
    11641 ctccatctac tctcatggct tccagccaag cggccgttct ttgtcccaca aaggttttat
    11701 tttcatttcc atctggagtt cctactacta ttactccaag taaatctaca tccttatcac
    11761 tttcaactat gtctaaaagt gggtctctaa agtgatgaag agatgtttct tttgttgaag
    11821 gtcctattcc cataatatcc acctctctta agtcatagct ctaagggcac catctctgta
    11881 ttcattagga gatataatca taggtacatt tcccatatct ataattgatg ttcctccttc
    11941 aaatccacta ggttcctttg agaataactg attgtcatac atagcacctt gacctgctat
    12001 ttgttttact atgacaacct tttttgcatt tggtcgaatt ttgtcaaaat attcacgaac
    12061 ttgagtagca tttcttccgt cttccatttt caaagacgct cttatttctt gtataaaatc
    12121 atcacatgct ctgaatgctg caagtggaag tgttctttca aaaggtaaac ctccctttaa
    12181 agttacatct acatggatta tataatcttc aactgatgga gttccatatc tcccaaatat
    12241 catttggtct ttcaaaattc cttctgaaga accaaattca tgcatttgat ttccatcttc
    12301 atctgcacca gttaacatta catatacacc cgttaaagta tgtgtaatac cctctcccag
    12361 tctgcctaaa acttttgttg atattggtat aatgtccata atagtattaa tttctctgtc
    12421 ataatctcca ggttttataa tgtctacttt tatgtctgtt attaactctt ctctttcttt
    12481 tatttcatct atacttgata aatctattgt aagtacatct ttctttatag aagtttttga
    12541 accgaaatta acttcattca tatgaaacgg ttttataaca agtcttctca ttattttttc
    12601 ttccataata tccacctttg ttcttaaaac ccctctccta accgtgtagg agaagggttc
    12661 tatattttca aatataaatt acattttaaa cgtgagcttt atattcgtat ggtaatggaa
    12721 ctatcttacc agcagatggt attgcaacta attgctctaa tgtatctctt aatatatgtg
    12781 tttgcatttc tatattatgt ggttcaccag cattagcacc cattggaact aatggagcaa
    12841 ctgctctagg agttccgttt tgtctaacta ctggaggaag agctgctatt attattgtag
    12901 gtattccagc ttcctctatt gctctctgca cgatcacggc agttctatgg caagtacctc
    12961 agccagctgt cataactaca ccgtctactc cctcttcctt aagtttagca gcgatagcag
    13021 gaccagtttc ttcagtgaag acatgttggt ttcctccacc acccatgaat gcatagtgca
    13081 taggagctac tcctttgata aatccttctg cagctaattc atgtaatcta tctataggga
    13141 acatacagtt tatatctttg tttacatctc cattgtcata tccaccatgt gataccatca
    13201 tttcatcaac tgtagctgta ttaggtatag ctctaaatgt agtatctcct gctagattaa
    13261 atctcttgtc agattttaaa tgaacaccag ctgctgtagc taaagctata gtcatatctt
    13321 ttaattcttt agttacagga gtccaaactg gaggaggtgt tattggaacg aatatttcag
    13381 attgaagtcc ttgtactgtt gtaaggctca tatatttcct ctcccttcta ttttcaaacg
    13441 attatttatt atttttcttc ttgttttgat tgcatttctg cttgtttttc tttttctctc
    13501 ctaatatttt ctacatactc ttgattaggc tctggactta atacctcagg atagcttagc
    13561 atgaatccaa cttcttgacc tatcttaagt tcgtagtcag ttataagcat tctattaggt
    13621 attttaaatt gtccaagtct accttttagg tctatagtca ctccaccacc tttgatgtca
    13681 actataatac cttcataaaa agtctctgat gagatatatt ttaacctatc cattataatt
    13741 ctacctctct atactgtttt aaacttggat ctaaatttat gttattgtct aaatcttagt
    13801 tagtatctaa attttagttt tcgtcttttt cgtatatttc tcttctcttc ttactcattg
    13861 gtagagattg ctcattctct tctaatacta ttttttctcc tgtaactttt tctatagctt
    13921 ctatgttatt taatttaaca tttggattcc attttctttc aggagcttta acttctacac
    13981 cttctatagc atttttaagc atagccatta ttctaacagc atcttctgga gctaaagtgt
    14041 tgttagataa tatttcattc tctatacctt gcttagactt attgttgtct accatgtgag
    14101 tcatgtattt attaccaaca actagagcac cttgaacagc tgagaaactt acaccaacta
    14161 ctggtatacc tctcatacct atttgctcta tgtgagatgc gaaatctata tggttgtttc
    14221 cgaaaccttc agtagttact acagctccat caacttccat agcttcaact aacataccta
    14281 atctcttaga tacatagaat ttctcagcat ttacttgtgg agaaccaaca aatacaacac
    14341 ctactaaatc tatttcttcg tcttccatag ctcttattac taatggctct ctccagtaat
    14401 gtcttgacat ttcttttgat gcaggtccta tacaagttaa tgcatggata ccaccatcta
    14461 atacttcaag tggagataat acaactggca tgtttcctaa gtcaacgttt ggttgagctc
    14521 ctaatgtacc aactggctca actggtaata ttaggttatc atgcattgca ccttgtccca
    14581 ttatttcttt tataacaaca actttctttt taccttctct tctgtattgc tctattactt
    14641 cttcatcaac tactaaagaa ttgtcaactt tctttaatgc ttctcttatt tcttgagtta
    14701 catagtcaaa tgcacggtga gcagctaaag gtcctggtct ttccatgtta gttcctgctt
    14761 ttattgttac ttgacctttg atgaatattt cacctttgtc agcagcacct ggtctacccc
    14821 acattatagt agtatttaac tcaccttctg aagaaccaaa ttcacctatt tgaactccat
    14881 cttcatctgt accagttaat accattacag ctccatctat aactctagtt atacctgaac
    14941 ctaattcgcc ttcttcttta gtagcgattg gttgtatatc taatacagct tcactgtaag
    15001 tttcatattt atcaggagtt attatttcta acttcatatc tacaactaac tcttgagttt
    15061 cattagcttc tttacatatt tcttctgcgt ttctgatgta taaagtagtt ccttctattt
    15121 tagtttcatc agcaaattca actttatcta ttttaaagtg ttttttagtt aagcttctta
    15181 aaagtttctt ttcttcaact tttggagcta cttctgctgc tgcacttgcc actggagcag
    15241 caacgtttgc tactggagca gcaactcctg cttgaccagc tactgataaa ggtatctcta
    15301 agcttatgtt cttaccttca cctatgtgta ttttaactgt ttcacctgca actcctgtgc
    15361 ttactggaac tactgctgct tctgatactg gagctgcttc ttctactgtt tcttctacta
    15421 cttcttcttt agcctctcct gctttgtatc cttctacgtt atctgtagtc attggagaaa
    15481 gtgcatctaa agtttctttt aacttagctc ctagtacttg acctatagtt aatccatttt
    15541 ctggtattgt taataatcca gaatcctcta agtctgcaaa tattgctgga tcttctaagt
    15601 tttctggcgc tataattgtt cccgcttcaa atctacaaca acaaactgct gggtcatttg
    15661 catgggcttg agctgtttct aaagttattg acatagtata aaatacctcc ttacattatt
    15721 ttcttattta ttattttaaa tctcaattcc tcaacaggta accccttaac tgagaaatta
    15781 agtaaattaa aacctacata atttgttatg attaatatta tttttttctc acttaaagtc
    15841 actttatatt ataagcaatt cctatgccaa cttattaaca aaaagatttt ttctataata
    15901 gattcttcat ctatcgctat ttttagccaa atattatata aatatttatt taaaatatgt
    15961 tattttttgt atttgtaaac attttctcaa acaaaaaaag tgtcaattaa tcaacacttt
    16021 ttcatttttt ttaacacatc tatatacaca acacttatac aagcgttggc tttactaggt
    16081 tttaaattaa agtttatcat tttgtcaaaa tttagtcatg tgtcatattt tagacacatc
    16141 tattttgtat tggtctatct tataatacaa agttgttctg ggtatattta atatcttagc
    16201 tgctttagct ttatttccat tagacatttt aagtgccttg gttatattct ttatttcaat
    16261 tttttgagtt gccttcgtta aatctaaagg atactcttct tcttcagtac tattatttgt
    16321 agaatccatc atataacttg gtacatcatc cacatctatt ttagaatttt gtgacaatac
    16381 aacaatattt tcaatcgtat tttttaattc tctaatatta cctttccatc tataattctg
    16441 aaatatatct attacatctt tgctaattgt aagaacaggc ttattatttt gtttacatat
    16501 ctcctctaaa aagctatgaa ccaataatcc tatgtcttct tttctctctc taagtggagg
    16561 aatctttatt tcaacaacat ttaatctata gtataaatct tctctaaatt tttctgcctt
    16621 taccatttta ctcaaatctt tattggtagc tgatattatt ctaggattta tttttattgt
    16681 ggtatctcca ccaactcttc ttatttcttt ttcctgtaat actctaagca actttgcttg
    16741 catactaaga ggtaaatcgg caatttcatc taaaaataca gttccatctt ttgcaagttc
    16801 aaatatacct attcgaccct ttttacttgc tcctgtaaat gcacctgatt catacccaaa
    16861 aaactcactt tcaaataact catttggtat ggcactacag ttaacaggta taaattgacc
    16921 ttttctctca ctataatcat gaatagctct tgcaaatact tctttacctg taccactttc
    16981 gccccaaata aatatactac tatttgttct agccacttgt tttgcaatcg ctttcgattt
    17041 ttccagttta taacttttac ctaaaatctt atcaaaacta cctttagata aatttttgac
    17101 ttcgttcttt agatatttta atgtatcatt tactttttca agttcaagag ctaatatctt
    17161 agcttctgta acatcaactt ctgtacatac aacaccgata aattcttctt tataaaatat
    17221 aggatttgca tgaactaatg catacatatt ttctttttta tctgtaaaat acaagtcact
    17281 cattccaact ttactattta gtactttttc tgaaatagta ttttccaaaa attcattcat
    17341 cggtcgacca actatatctt cagactttat atcatacctt tcctccataa acttattcca
    17401 caagataaca acaccttcgt tatttatagc acaaattcct tctttaattt gacctataat
    17461 atacttcaaa gtcaatccat aatcttctaa atgcatatat agatagtccc taatgtgctc
    17521 ttgttttaga actccaataa ttttattatc tctaagaaca ggaagaatcc ctatattttt
    17581 taatatcatt atatctctac attcatctaa ggttaaacct tcgttgacat atattacatc
    17641 cttcttcatt ataagtttta ctggttgacc ttcatacttt ttatgcatct cataaagatt
    17701 atgtatatcc gtcatggata taatgccttt taattggtca gatgaatcta ttaccattag
    17761 agtttttgtg ttgcttttta tcatttcttt tattgcatct tcaattcttg tgtcttcgtc
    17821 tatagttgta aattttgtat ccattacctc ttctactttt tttacttctg gtatcgaaaa
    17881 cataaagtcc cccctttata gtagtacctc atcaaaactt atttatatat atttattcta
    17941 gcatattata ataattattt attaaataat tattattaaa atttattaag taattagcat
    18001 ttaaatttat ataatcactt taaaatttat aattaaattt atacaaatga aatcaaatga
    18061 attataccct tagtatatta ttttttcaat actattttca ccttcaaaac atttttctat
    18121 tcatcaaa
//
LOCUS       DAEMHW010000069        17857 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEMHW010000069 DAEMHW010000000
VERSION     DAEMHW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17857
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.069"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            319..1248
                     /gene="glsA"
                     /locus_tag="KPK67_001955"
     CDS             319..1248
                     /gene="glsA"
                     /locus_tag="KPK67_001955"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBG2458924.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            1346..1909
                     /locus_tag="KPK67_001956"
     CDS             1346..1909
                     /locus_tag="KPK67_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2458925.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            2397..3233
                     /locus_tag="KPK67_001957"
     CDS             2397..3233
                     /locus_tag="KPK67_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBG2458926.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKKELKMIKEILS
                     SK"
     gene            3515..4510
                     /locus_tag="KPK67_001958"
     CDS             3515..4510
                     /locus_tag="KPK67_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG2458927.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(4767..5171)
                     /locus_tag="KPK67_001959"
     CDS             complement(4767..5171)
                     /locus_tag="KPK67_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG2458928.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            6190..7104
                     /gene="queG"
                     /locus_tag="KPK67_001960"
     CDS             6190..7104
                     /gene="queG"
                     /locus_tag="KPK67_001960"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBG2458929.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            7210..9447
                     /locus_tag="KPK67_001961"
     CDS             7210..9447
                     /locus_tag="KPK67_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2458930.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            9644..10696
                     /gene="nth"
                     /locus_tag="KPK67_001962"
     CDS             9644..10696
                     /gene="nth"
                     /locus_tag="KPK67_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBG2458931.1"
                     /translation="MKNVNLKEINEKDVIVEEDINLKGMDKKDVIEKEEVNKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKST
                     KKGETEKKTKKSSSSKATQKQSKEVKSKSKIKNESANEKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            10890..11360
                     /gene="trmL"
                     /locus_tag="KPK67_001963"
     CDS             10890..11360
                     /gene="trmL"
                     /locus_tag="KPK67_001963"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBG2458932.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            11396..12244
                     /locus_tag="KPK67_001964"
     CDS             11396..12244
                     /locus_tag="KPK67_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG2458933.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            12617..13621
                     /locus_tag="KPK67_001965"
     CDS             12617..13621
                     /locus_tag="KPK67_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2458934.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGVKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSV
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            13747..14859
                     /locus_tag="KPK67_001966"
     CDS             13747..14859
                     /locus_tag="KPK67_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2458935.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSVTEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            15349..16110
                     /locus_tag="KPK67_001967"
     CDS             15349..16110
                     /locus_tag="KPK67_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458936.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            16113..16931
                     /locus_tag="KPK67_001968"
     CDS             16113..16931
                     /locus_tag="KPK67_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBG2458937.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            17111..17713
                     /locus_tag="KPK67_001969"
     CDS             17111..17713
                     /locus_tag="KPK67_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBG2458938.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT         7367 a         1619 c         3284 g         5587 t
ORIGIN      
        1 ttattgaatt actaaattac taggttgtta agttactgga ttattagata gttagattac
       61 taggttgtta aattattgaa ttattagatt gctaaattat taggttgtta aattactgga
      121 ttattagatt actaaattgc taggttgtta aattattgaa ttattaaatt attaagctat
      181 ttaaactatt taaattgttt gaaaaatgaa ttgattcttg tagaaaataa ttttaaagta
      241 taattttttt taacattgtt ttattatagt ataaacttgt gctaaaatag aatatataac
      301 ataatataag aggtgcatat gaatacgtta gatgagacat tattaaaaga aattataagt
      361 tctaataaga agtatactaa ttatggacaa gtcgctagtt acataccaga attaaaaaac
      421 gctagaagaa atgatttagg aatttgtatc atagatagcg aaaataatct ttatagtgct
      481 ggaaactgta gcacaaagtt tactatacaa agtatttcaa aaccaattgt gcttgctatg
      541 gcacttatgg ataatgattg ggaagacgtt ttttcaaacg ttggaatgga gccaagtggg
      601 gacccattta attcaataat gaaacttgaa attaatgaca caaaaaaacc ctgtaatcca
      661 atgataaatg caggggctat agtaaccact tcccttataa atgggagttg tttagaagaa
      721 aaagaagaaa gaatgcttag tttctttaga aagttagcaa aaaatgataa tataggaata
      781 aactatgatg tctataagtc tgaaaaaatg actggagata gaaacagagc tatggcttac
      841 ttattgaaaa gtgatggatt tatcagaggg aatgtagaag atgttctaga cctttatttt
      901 aaacaatgct caattgagat agattctgta gatttagcga gaattggaat aaatcttgca
      961 aattatggag tagatataga aaatggtgag catttaatga gtgaaatggt ttctaggatt
     1021 gtcaagacat ttatgatgac ttgtggaatg tatgatgctt ctggggaatt tgctattaaa
     1081 gttggcattc ctgcaaaatc tggagttggg ggtggtataa tggcttctgt gccgggacgc
     1141 atggggattg gtgtttatgg acctgcgctt gacaaaaaag gtaacagcgt cgcaggagtt
     1201 aaagttttag aggagttgtc aaataaactc aaattaaata ttttttaaaa tattaagaaa
     1261 ggattttttt gacaacaaca tataatttaa tatataatta aattgtaaag aaatatgaat
     1321 taaataaatt ttttaggagg ggtcaatgaa agaggtattg gtattacgag atttggagtg
     1381 cataaaggct attgcgcatc cacgtagaat agatattcta aaagcatttg acaaatcgcc
     1441 attgtcagca aaacaattat ctcaattatt ggaagaacct catgcaaaaa taaactacca
     1501 tataaaaatg ttatacaaag ttggtatttt agaattagtt gaggaaaaaa taaagtcggg
     1561 gattgtggaa aagtactatt atccaagtgc aaaaaatgtg gttataggga ataggatact
     1621 taatttttct ttagacaatg gggaagaaaa agaagaattg tacatatcaa aatttgaaaa
     1681 tatgagtgaa gttttttata aggcagcaga ggaagatgtt ttagaaaatg aaaatatagt
     1741 tgattatcat gatatttctc ttactcatga tgaattagtt gaacttagtg acactatgaa
     1801 gtcaaagata gatgaaattt taaataaaag acaacacaat gttgaaggtt ctaagtatga
     1861 tatagctatg gttatagttc ctactcttga ggaggaatgt cctagttaga gaattaatta
     1921 tggattaatt aaagatataa tattagagat tgaacatcaa aatttactta ataatcaaaa
     1981 aatagttttt ggtatgggta aaacaatgat ttttaatctc tatttttttg ttttcttata
     2041 cctattgtat ttgtaaataa tatgtatagt gtaagatgta tacactatct aagaagtgct
     2101 tagataatat acaaagtatg taagattata atatttgaaa ttgattaaaa tgggtataat
     2161 taaagtgtaa atattaaaat taaaaattaa tataaattaa atcaatgcta gaaatatgtt
     2221 aaaagaggaa tgagattagt tgggatagaa catcccttag agcttatgaa tttttagtga
     2281 aaaagtacta tcaaatagga atctccatct tcatatattg gctaaatgta gttaaggtgg
     2341 agtagttcaa aattaaaatt gagtacaagt agtagtacaa ataaaaggag attaatatga
     2401 ttaaattagg aacacatctt tcaatagcaa aaggatttgc taatgcggct gagacagctg
     2461 tatatattgg tgcaaataca tttcaatttt ttagtagaaa tccaagagga ggaaatgcta
     2521 aagaatttga tgaaaaagat ataattaaat ttcaagaaat tagaaaagaa aataattttg
     2581 caccactgct tgcacatgca ccatatacta tgaatctggg tggtacaaag gatgatgtat
     2641 acgaatttgc aacaagggtg atagatgagg atataagaag gatggactca ttaggaattg
     2701 aatacatgtg ttttcatcct ggaagtcatg taggtggtgg tgtagatttt ggtatagata
     2761 gaatagtaaa aggattgaac aatgccataa agggtgatga aaatataact atacttcttg
     2821 aaactatgtc aggaaaggga actgaaatag gttttaaatt tgagcaatta aaaagtataa
     2881 tagacaaaat tgagcataaa gaaaaaatag gtgtgtgtct tgatacatgt catatttttt
     2941 cagctggtta tgatatagtg aatgatttag atggggtttt ggaagaattt gataaggtta
     3001 taggcataga aagattgaaa actgtccatc taaatgatag tatgatgcca tttggagaca
     3061 aaaaagacag acatgctcct ataggggaag gaaagatagg attaaaagca ttaattgatt
     3121 ttatggaaca tcctagtcta aaacatttac catttttctt ggaaactcct tttgatgatg
     3181 aaggtcacaa gaaagagctt aaaatgataa aagaaatact atctagtaaa taaaccttta
     3241 ttaaaaatat gtgaaatact ccgccctcaa cataattagg gtggagatac aattatgaaa
     3301 tgtgataatt taaattaatg gatagtaata acaaaaattg tatacaaatt aactgggtat
     3361 aaagtatgat tatattatga atagactgat ataaaatata attatattat gaatagtatg
     3421 taaatatatt gcaaagattc tcataatttg ttgtataatt aaagatgaca tatatattat
     3481 ataaaaaatt attaaaagtt aggtggggaa agatatgaga aatttaggca atactaacat
     3541 gaaaataaag agagttggct ttgggggcat tccaatacaa aggattactc aagatgatac
     3601 taacttagtt ataaatgagt tagaaaaaca aggaataaac tttattgaca gtgcaagagg
     3661 gtatacaata agtgaggaag caatcggaat tgccattgaa ggaaaaagag ataaattttt
     3721 cttagctact aaaagtatgt ccagagatta tgattcaatg aagagagatg ttgaaataag
     3781 tttaaataat tttaaaactg attttataga tttatatcaa ttccataatg ttaaagaaga
     3841 ggagtatgat aatcttttta aagataaaat ggcttatagt gcccttttag aggcaaagga
     3901 gcaaggtaag ataaaacata ttggtataac tagtcacaat ttaaatacta tagaaaaagc
     3961 tatagaagat ggaaaatttg acactataca attcccttac aatatagttg aaggacaagc
     4021 agatgaagtg tttaaaaaag ctcatgaaaa aggaattggg ataatagtaa tgaaaccttt
     4081 agctggaggt gctttagaca atgcaacact ggctattaaa tatatactat ctaaagatta
     4141 catagatgta gttataccag gtatggagag tgttgaacag gttagacaaa atgtcgctgt
     4201 acttgaaaat cttgtgctag atgaaaaaga taataaggaa atcgaagaaa taagaaattc
     4261 tttaggtaaa aaattctgta gaagatgcga atattgtatg ccttgtgctg taggaataaa
     4321 tataccattg agttttttat gtgaaggtta ttatactaga tatggattaa aagagtgggc
     4381 taaagaaaaa tatgaggtta tggatgttaa acctactgag tgtattgact gtgggctttg
     4441 cgagagtaga tgtccatatg aacttcctat aagagaaatg cttaagactg ttgttgaaaa
     4501 gctaggatag attttattta ttgaaatcgg tgtattaaat ttactccgat ttctttttat
     4561 tagatgtggt cttatataca aatattaaat ttacatagtt atagctatta tataaattta
     4621 atatttgttt aatacttatt aaatatttac ttaatgcttt ttaaaaacat ttgtttgagt
     4681 atagacataa cacaattatt attgttgtat tagtatagtt tcaaatactg gagtatcaat
     4741 ataatgtcga ataacacata aaatctttat ttttcaaaaa tattatagga tatatcgtat
     4801 ttagatactg gataaagttc gatttcaacc ctagggttat ctttatctat aaaatcaaat
     4861 aaaagtactg gcttaagttg tttatcattt tttattattc cacttttttc aataccatca
     4921 caaatacttt ttggataatt atgtaaatcc cccattctct tatttgggaa gaataatttc
     4981 aagactgtta ttagattttc ttcaacagtt tctccttgat actgttggtt tataaaacct
     5041 gcaatcatgt tttcgtatgc aagatactta gaatattttc cctttgaagg catccatgct
     5101 tgaccattta tgttatgaag cttaaaatta gatttactaa taggtctacc tggaattgtt
     5161 attttaatca aatttttcac catccttata cagtatttat tataatctgt atatgattta
     5221 aaagcaaatt gttatttaaa gtgtttttac agagcgtgtt tgtaaaattt tccactttag
     5281 atgtagtaat ataagataag tattttgctt acaaatttta aaatttgata tatgatggac
     5341 atattcatat tagactatta taagaccttt tttagtaaag agagtaaaaa gaactccata
     5401 taagtcaagt aaagacttaa taaatggcaa tgtaaatggg atattaatta taagaaaaaa
     5461 tcaatctttt tgcattaact ataggaagaa tgtcaaaata taactaatga tatttgaagt
     5521 tttagagttt atattaaaag gtaagaaggt taagtgggta aaaaaggttg aatagattaa
     5581 aaaggctaag tatctaaaag agttagtagg taaaaaagtt aaataagtaa atgaaaaagg
     5641 ctaagtatat aaaggagtta gtagataaaa aaattaaata gataaaaaga ttaagtatct
     5701 aaaagcgtta gtagataaaa aaattaaata aataaaaaag actaagtatt caaaagagtt
     5761 agtaggtaaa ataattaaat agataaaaag actaattatt taaaggagtt aagtagataa
     5821 aaaagttaaa taagtaaatg aaaaagatga agtatctaaa agagttagta ggtaaaaaac
     5881 taaataggta aaaagatgaa gtatctaaaa gagttagtag gtaaaaaatt aaaaaggtaa
     5941 aaagacgaag tacctaaaag agttagtagg taaaaaaatt aaatagctaa aaagacgaag
     6001 tatctaaaag atttaagtag gtaaaaaaat tgaatagcta aaaagattaa gtatttaaaa
     6061 gagttataaa aaagttaaat aaataaaaaa gacgaagtat ttaaaagagt tagtagctag
     6121 aaaaggttaa aaagactaaa aaaattaaat aaatttaaaa agtcaattag attaaaaagg
     6181 aataatttta tggataataa ggaattaaaa gcattttgta atttaatagg attaaactgt
     6241 gtaggagtag caggggtaga taaatatgat aatttagaaa agatacttaa ggacagacaa
     6301 caaaaaggtt atttaactgg tatggaagaa ccaataattg agaacagaat taacccaaga
     6361 catataatga aggatgctaa ctctataata gtctgtgcat ttccatatca tataaataga
     6421 caagaaaaga ataaaaattc aaatttatca aaatattgtc atggaaagga ttatcacatt
     6481 gtagtaaagg actttttaca acaaatttgt gattatattt ctaaaacagt aacagatttt
     6541 aaatacaagt tatttgctga taatggacca ttagttgaca ggtatttagc ttatttatca
     6601 ggaataggat attttggaat aaataataat atcataactg atgaatatgg ttcttacgta
     6661 tttatagggt atatagtaaa taattataaa tttgaagaag ataaaccact agaaaaaagt
     6721 tgttttaaat gtggtaagtg tgtaaaatat tgtccgggaa atgctatatt gggaaattat
     6781 gatatgaacc ccaaaaaatg tttatcatat ataactcaaa aaaaaggaga tttgtcagag
     6841 aaagaaaaag ttgttttaaa gaatggcaaa aaggtatttg gatgcgatat ttgtcaagaa
     6901 gtatgtccac ataatgttga aataccaaca actcatattt tggaatttaa agaaaatata
     6961 atagattatt tggattatga tgagatacaa aatatttcaa ataaagaatt taaaaggaga
     7021 tatggagata gagcttttag ctggagaggt agaaatataa taaagagaaa tatggaaata
     7081 attttagata agtatgatga ctaataataa gagtctgttt catatacttt aatgtgtatt
     7141 tactttaaca cttgttgtgt aaaagtcata ttatatcatt aaagttaaat atggggaggt
     7201 gggggaatat tgaaatataa taatacatct gaaataaatc cactagatta tatattgggt
     7261 caagaaagag ctgtagaagc tatggaatta gggttgaaaa taaataatcc agcgtacaac
     7321 atatatatag ctggtgaacc tggaactggt aaaagtactt atgccttaaa ggttttaaat
     7381 gagtatgcat ccaaaaaaaa tacgcataaa gattggtgtt atatatataa ttttgaaaac
     7441 cctagagagc ctattatagt tgaattagaa aaggggtttg gaagagaact taagaaagat
     7501 atggagaaat taattgaaag tcttttagaa gattttaaaa atgcgtttga aagtgaaaat
     7561 tttgaaatag aaaaaaataa acttctggat gaatatgaaa tagaaaaaga tatgcttttg
     7621 aagcaaatta agaaatatgg agaagaaaaa ggatttaaat taaaacagag taaaatggga
     7681 atagttttta tccctttgaa agaagaggaa gatgaaagtg atgagtctga tgaagagttt
     7741 tacaaagcta aaagagaact agaaaatatg gcgatacagg tcgtatataa aattagaaac
     7801 ttagaagaaa tagctgaaaa agcagtgtta gagcttgaag aagagatagc taaacttgta
     7861 gtagaacctc atatcaagag attgtgtgaa aaatatgaaa attatgataa gattcaaact
     7921 tatctggaaa atataaagaa agatataata gaatatatgt atttattcta tttagatgaa
     7981 gaggagttaa aagataagta tgataaagag cattttatta aatataaaat taacctattt
     8041 gtagacaatg gaagctcaaa gaataaagaa tcagcacctg ttgtagtaga gataaaccca
     8101 agtccagcaa atctatttgg aaaggcagag tatgattatg caaatggaaa tataaagact
     8161 gatttcacaa aactattatc aggagcattt catagggcta atggtggtta tttagtactt
     8221 tatgcagacc aattactaaa atatacaatg tcttgggaaa ttcttaaaaa gactttgcag
     8281 actaaaaaag taatattgga aacacaaaca gcaattaaac cagaaaatat gccattggat
     8341 gttaaattag tactgattgg aagccactat atatatgata ttttatatag atatgatgaa
     8401 gattttgaaa aatattttaa agtatttgta gactttgata gtgaaatgga taaaaatgaa
     8461 gataatgaag aaggaatagc aagatttata gcataccaat gtgataagaa taatttaagg
     8521 cattttacta aatcagcagt tgaagaggtt attaaattta gcactagatt atctggtgat
     8581 ttagaaaaac tatcaactaa gtttaataaa ataatggaag ttgtaataga aggaagtgct
     8641 tatgcagagt ttagaaattc ggagtataca gagcagtgtg atgttaaaaa agcaataagt
     8701 gaaaagagaa aaagaatcaa tagagtggaa acacatatgg atgaatcaat tgaaaatgga
     8761 tttactctaa tagaaacaga gggaagaaaa gtaggagtga taaatggttt gtcagtctta
     8821 agtacaggag aatactcttt tggtagagct tcaaggatta cagcgaccac tagcccagga
     8881 agtaaaggca tagtaaatat agaaagagaa gtaaatatga gtggctctat acataacaaa
     8941 ggagtactta tattaggtgg ctatctttct gagaattttg cacaggattt actattgtca
     9001 ctaaatgcat atgtttgttt tgaacaaaat tatggtggaa tagatggaga tagtgcatct
     9061 tctgcagaat tatatgtact attatcatct cttagtggag tatcaattaa acaaaatatt
     9121 gctgttacag gttctataaa tcaaaagggt gatatacaag tagttggtgg tataagtgaa
     9181 aagatagaag gattttattc tatatgtaaa actaaagggt taaatggtga gcaaggtgtc
     9241 ataattccta gaaaaaatca aagaaatctc gttttatcag atgaagttaa tgatgcagtg
     9301 cgagatggaa aatttaagat atatactgtt gaaagagtag aagaggctat agagatattg
     9361 acagatgtta aatttgaaga aataaagaaa ttggtaaaag aaaaattaat atctttcagc
     9421 aagatacaaa ctgtgtcaaa agaataagtt tattatagaa tttaatgggt atttatgtat
     9481 aataaaaagc aagaacttta ataggttttt gctttttttg taaatatata taaattaaat
     9541 tcttattttg tatgttttat atattgatgt ttattttttg ttgacatgat taaactattt
     9601 ataaatagcg ttttataaat tagtatttta aagggggata attatgaaaa atgtaaatct
     9661 aaaagagata aatgaaaaag atgtaattgt ggaagaagat ataaatctaa aaggaatgga
     9721 taagaaagat gtaattgaga aggaagaagt aaataaaaaa agtatagata agaaagatat
     9781 aattgagaag gaagagatag ataaaaaaaa tgtaaagaag gtcgaggttc aaaaaggaat
     9841 agataaaatg attggtaaaa aagatgagat aattgatgag aaagatatag agaattttat
     9901 ggatattgaa gaagataaaa aaagtatcaa agtaagtaaa aaaagtacta aaaaaggtga
     9961 aacggagaaa aaaacaaaga aaagtagttc tagtaaggct acacaaaaac aaagcaaaga
    10021 agttaaaagt aagtctaaaa ttaaaaatga gtcagcaaat gaaaaagacg taaataagat
    10081 tttagacgag ttagaaaaat tgtacccaga tgctaaatgt gagttaaatt atgggacagc
    10141 ctttgaactt ttgatagcaa ctattttatc agcacaatgt actgatgtaa gagtgaataa
    10201 agttacttca gagttgttta aaaagtacaa cacagctaga gactttgcta atttgagcat
    10261 tgaagaaatt agtaaagaga taaagagttg tggtctttac aaaagcaagt ctcaaaaaat
    10321 aaaggataca tctgaacagc tatgtgaatt atatgatggt gaagtacctg atagcttaga
    10381 aaagcttata aaattacctg gtgttggaag aaaaacggca ggggttgtac ttagtaacgc
    10441 ttttaatcat ccagcaatag ctgtagatac tcatgtattt agagttagca atagaatagg
    10501 aatagtggac gagccaaatc ctcaaaaaac agaatttgca cttatggaag ctataccaaa
    10561 agaaagatgg tcacactcac atcatgtatt aatttttcat ggaagaagaa tgtgtaaagc
    10621 tagaaatcca gaatgtgcaa gttgcccaat aaaagaagat tgtaattatt ataaagaact
    10681 taatgaaacg aaataacttt tagaaagtaa gttgtacaag agggattttc tgatataata
    10741 atacttgtga aaatgttaga taaaaaacta ttatatacat gaaataaaaa ttattattaa
    10801 cattatttta tataatagta taatattatt gttattctgt ttataaaata aaatttgata
    10861 aataaaagcg atacttaggg aggattaaaa tgtcattaaa tatagtatta gtagaaccag
    10921 aaatacctca aaatacagga aatataataa gaacctgtgc agcaacaggt tcaactttac
    10981 atttagtaaa accactgggt ttttcattgg atgataaaca cctaaaaaga tgtgggcttg
    11041 actattggga tatagctgat atacaatatt acgatagctt tgaagaatta caagaaaagt
    11101 atccaaatag taaatacttt ttttctacaa caaaggctaa acagtcacat tcagatgtga
    11161 aatatgaaga aaattgtttt atagtatttg ggaaagaaac aaagggatta ccagaacctt
    11221 tgttgaaagc aaatatggat acttgtatta gagtgcctat gttggatgtt gaaaaagcta
    11281 ggtcattaaa tttatcaaat tcagttgcta ttgtagtgta cgaggcttta agacaaatag
    11341 gatacccaaa tatgaggtag tttcaacaag cctaaatgag aaaggagcat acttaatgaa
    11401 aaatctaaag ataatctgtg acagtttatc ggacgtgcca aaagatttgt tagataaata
    11461 tgatatagat atagtttcat taacagttat actggaggaa agagaataca aggatagaat
    11521 tgatatatct ggagatgaat tttataaaag gcttagagaa gaaaatgtat atccaaaaac
    11581 ttctcaagct acttatgccc aatttaagga aatttttgag aaatatacaa atgaggatag
    11641 agagatactt tatatagcag ggtctgccac agctacaggt acgtatcaaa gtgctgtaat
    11701 ggcaaagaat gatgtagatg gtaaaatata tacatatgac tctaacttgc tttgtatggg
    11761 aactggttta cttgtaataa aagcaggaga acttgcaagt gaaggaaagt ctgttgctga
    11821 aatattgcct gttttggatg aattaaaaga gaaggctttt gttatatttt cagtagatac
    11881 attggaatat ttacaaaagg gaggtagaat atcatctact aaggcagcaa taggaagtat
    11941 attaaatata aaaccgattc ttgaaataag agatggatta gtttcgcaaa tatctcaagt
    12001 aagaggaaag aagaatgtaa tttctaagat gatggaattg ataaaatcaa attgtggtga
    12061 tgatttatca gaccaaattg tgtatatagg atatagtgat gattttaagg aaaaggaaca
    12121 attatctaag gttatgcaag atgaattaag acctaaagaa attagatttt tccaaattgg
    12181 tgtgtgtata ggtgcacatg ctggtcctgg tgttactggt atcatatgct taaaaaataa
    12241 ataatatatt tttgggtgac attatttatt tttttattaa attaggatag aatattaatt
    12301 attgagatgt taaattggat ttattttgga tgaaattaag tgttgatata ttagatttag
    12361 tttgtaggga agtgtgtaaa tagataagaa tctattgcac acttttttat agtaagattt
    12421 attaaaaaaa tttcaaaatg tacttgtaaa ttgtatataa aaaaattata aataggatgt
    12481 aaaagtaaat aggatgtaaa agtaaatagg atgtaaaagt aaataggatg taaaagtaaa
    12541 taggatgtaa aagtaaatag gatgtaaaag taaataggat ataaaaatat cgtaaatgta
    12601 tgaggagtga aatttaatgc gtttcaaaag aatattgata ttgatgttaa ctgtagcaat
    12661 gatagctgga atgttggttg ggtgtgctaa taacccaagc aataatccta gctctagcaa
    12721 agataataaa agtgatataa gcgataaaag taattcaaaa aaatctgtat caatgatact
    12781 agatattgaa ggaacaaata atgaagctat gaataattca gctttattag cattaaacaa
    12841 tgctcaaaag aaacttaata tagatacaaa caaagtggaa tcagatgatt cctcaacttt
    12901 ttctaatagt atagatattt tatgtaatga caattacgat ttaattatag ctgtaggagc
    12961 tagatttgca aaaccattgg agatggtagc caagaaatat ccaaagcaac aatttgcaat
    13021 tatagactat gagtatgaca agcaaccttc taatataact tcaatttctt atgaagataa
    13081 taaatctgga tacctagctg gattaattgc tggaaaaatg actgaaagcg ataaggttgg
    13141 ttttatagga ggagtaaaga gctctagtag ggataaattt gaatcaggtt ttagagaggg
    13201 tgttaaattt tctaatagta gtataaagga tatttcagtt gagtatgcag atgtttttaa
    13261 agatagcaaa tcagtagaat ctatagctaa aaaaatgatg gataatggag tggatattat
    13321 tttttctact actgaagatg attcaaaagc tgtaatagat gcagtaaggg caaaaaataa
    13381 aaaagttata gcaacgaata aagaccaaca tgagttagct ccagaaaatg ttataagttc
    13441 tgttgtaaaa gattttgaga atcctactta taatctgata caaagctttg taaaaggaaa
    13501 ttataaaggt ggaaaagtta tagaaaacac agttaagtct ggaagtactg ggcttgccca
    13561 aaatagttct caaaatattc ctccagatgt gttagaatat gttaataaga acaataaata
    13621 atctactata atatccaaga atacttatga ctttagttat gaggtgaatt ggatgttata
    13681 atagacatat atagaaagac atatgcagag gtaggtcaga cctatctaaa tcagggagtg
    13741 aattttgtga aaataaaaag gatattgatg gcttgctttg tagtagtttt tacaattttt
    13801 atattagcta tatgtaattt tgattttggg tttgataaag taaataagaa taattctaat
    13861 atagatagtg ctaaatcgaa acagagtgag tctaaaagtt tgccaacttc agcagaaaag
    13921 gataaatcaa atagtaaaag taagaataaa gaagatgaca cagaagatga ttcatatgta
    13981 agaaaaacta ttggagtatt gttagatacc gaaaacttaa ataacgaatc atataataac
    14041 aatgtactat cagccttaaa aagggctgac gaagattttg atgttaggat aaaacttgaa
    14101 aaaccaaaga cttctaaaag ttttaaagag agtatagaaa aactgcataa gtatgaacca
    14161 gatttgataa tagcagttgg agccagattt gcagagccaa ttaaagaagc tgctactaaa
    14221 tatcccaaac agcaatttgc aatagttgac tatacttata aagttcagcc agctaatgta
    14281 atttctgtat catttcaaga taatgttact ggatatttag caggacttat tgcaggtaaa
    14341 atgactgata ctgggaaagt cggttttata ggaggagttg agggttctag tagagataag
    14401 tatgaatctg gatttagaaa ggggcttaaa acttcaaata aagatgctga gttactagtt
    14461 aagtattcaa atatatttga aaaccctgaa tcagctagaa ctattgcaaa ggaaatgaga
    14521 gattctgata gagatgttgt attttcagtt acagaagatg atagtaaaag agctattcag
    14581 gtagcaagag aaaatggtgg taaagttata gctattaata aagaccaata cgatatggct
    14641 ccagataatg taataagttc aattataaaa gatgtagaac aacctgtcta tgaactgata
    14701 aagaacgttg caaaagatgg ttttaaggga agtaaagtaa tggaatttaa aataaaagat
    14761 ggagaacttg gcttgacttc aaagagcagt agaaatatac caccagatgt tttggaatac
    14821 ataaaaaaag agtataacaa agtgaaagat acatattaat tattgatgta gaagattata
    14881 aaaatatact gcttaagttt agattagcaa taaaaagata tatttattaa atttgtgcta
    14941 actgtacaaa atatattgat taagtttaga ttagtgataa aaagatatat ttattaaatt
    15001 tgtgttaaca gtatggaata tactacttaa gtttagattg gtgataaaag gatatattta
    15061 ttaaatttag tctaactgta caaaatatat tgattgagtt tagattagca ataaaaagat
    15121 atatttatta aatttgtgct aactgtacgg aatatactgc ttaagtttag attagtgata
    15181 aaaagatata tttattaaat ttgaaccaac tgtacaaagc tatactaatt aagcttagat
    15241 taactatata aaattataac aataagtgtg tgggcagaaa aattctgcta cacactttat
    15301 ttatattcac ataaaaaatt tatcaaaaaa aagaaaaagg agtaaaaaat ggaatttaca
    15361 agagaaccat ttatactaga tgacgatttg tgtctagctt tggtggacaa aagaattagt
    15421 tatctaatgg aaaatgaatt aaaattaaga aatatagaga taataaaaac aatagaatgt
    15481 aaagaattat atgaaagcat aaaatatcac ccggatatat gtatttgtaa tttaggaaaa
    15541 ggagatataa ttgttgcgcc gaatgtatat aatcaataca aagagttatt aagtagatat
    15601 aaatttaata taattaaggg agaagctaat ttattaaata gataccctta tgatatacct
    15661 tacaatattg cgatagttgg tgactatgct atacacaatt ttaagtatac tgacaaagca
    15721 atattagatt atattgaaaa taataaactt aaaaagataa atatagagca gggctattct
    15781 aaatgctcaa tctgtatagt agatagaacg tctataataa catcagataa aggtatttgg
    15841 aaaagtatga agaatacaga aatagagtgt ttattgatag aaaaaggtca cataaattta
    15901 tttgagatga attatggatt tataggtgga tgtacagggt tgatttctaa ggataaacta
    15961 gctttttgtg gagatgttaa aaaacatcct gactatgaac gtataaaaag ttttgtagaa
    16021 agtaaaaaca aggagatagt tacacttagc tgtgaaaatt tactagatat aggttctata
    16081 gttcctctca tgacaagaaa ggagcgataa ctttggttga gaaggataat atttcttcca
    16141 tgaaattttt gaggaataat aatgtaaacg atagattaat atatacgaat gacattgtag
    16201 aagtagtgat agattctgca aagcaagaag tgttaaataa aaaaatactt aatggtacta
    16261 aagctgaaca aagagcttta aatattgatg agatatcaac ggaaataact agtatgataa
    16321 taagtataat gaaagagaaa cttttgaaag aatacattct aaaatgctat gggcgtacat
    16381 atcctgaaga taaaaataat atatacatat attcactgaa catatttgct aaaaaagaga
    16441 tttttatgag agaaacagta tttactaggg tgagaaatta tatagaagaa aatgatttta
    16501 taaatgtaga aggttttatt agatttagaa tgagagaatt tatgaaatat atttctgcta
    16561 taggtgatat tgcagttgag gagtatttaa taaagaaaga ccaagatgaa tttataagag
    16621 ttttaaaata ctttatagat actcaagaag aaaagattga tttgcttaaa gttcatataa
    16681 tggaagataa tacatttgtt ttatacgata aaaatggaaa taagatagac agtatagatg
    16741 atgaagaaat tataaatatg gtaataagag aaaatttaaa ttatgaggac tttttaataa
    16801 gtaccttatt aactctatgt cccaaaaaaa tagaaatatt ggatttgtta aataataatt
    16861 gctcaaagga aattatagat actgtagaag caatttttga aaataaagtt agtataatta
    16921 tggaaaacta gaatctaagc atataaaagc tagccatata aaattaaaaa aattcttttt
    16981 aaatcgacac aaaagactta taattgtata taaagctata gtataactag agttattaag
    17041 tataaagaat atctacttga ttaatgcaat atagctggca aatatacaat tgataaggag
    17101 taattttata atggataatt taattaggat actgatatta gctgttattg gtggatttat
    17161 aggttatgtt acgaatgtag ttgctataag actgatattt agaccaatag agcctattaa
    17221 aatacctatt ttaaatatag aaatagtagg tcttatacca aagagaagag cagaaatagc
    17281 agcaaatgta ggagaaataa ttcaagaaga atttctttct atggacgaaa tacttgcaaa
    17341 tatcattact gacgaagata aggaagaagt tgtaagatac ataaaagcaa gggtaaaaat
    17401 tataatacat gagaaagttt catttatccc gagtggtata aaaaacatga tacaagatta
    17461 tctaggagag ataattgagt ctgaagttaa gcaaagtata gatgaattga gtaaaaatat
    17521 aataaataaa gcaaatgaac gtatagatat acaaaaaatg gtcgaagata agattaatga
    17581 actagattta tatgaattgg aagagataat aataagaata gctaagaaag aattaaaaca
    17641 tatagagttt ttggggcttg tcttagggtt tttaataggt atagctcaag ggttaattac
    17701 tatgtttatt taaatatatg agaagtagca tttatttaag tactaattat atttacctat
    17761 ttgagtgaat tacttattaa taacatttag atattaatta tatttaccta tttgagtaaa
    17821 ttacttatta atagcattta gatattaatt gtattta
//
LOCUS       DAEMHW010000070        17628 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEMHW010000070 DAEMHW010000000
VERSION     DAEMHW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.070"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            208..1329
                     /locus_tag="KPK67_001970"
     CDS             208..1329
                     /locus_tag="KPK67_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458939.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            1347..2222
                     /locus_tag="KPK67_001971"
     CDS             1347..2222
                     /locus_tag="KPK67_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBG2458940.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            2246..2938
                     /locus_tag="KPK67_001972"
     CDS             2246..2938
                     /locus_tag="KPK67_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2458941.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            2946..3770
                     /locus_tag="KPK67_001973"
     CDS             2946..3770
                     /locus_tag="KPK67_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG2458942.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            3775..4842
                     /locus_tag="KPK67_001974"
     CDS             3775..4842
                     /locus_tag="KPK67_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBG2458943.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            4844..5380
                     /locus_tag="KPK67_001975"
     CDS             4844..5380
                     /locus_tag="KPK67_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG2458944.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            5448..6053
                     /locus_tag="KPK67_001976"
     CDS             5448..6053
                     /locus_tag="KPK67_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBG2458945.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            6065..6979
                     /locus_tag="KPK67_001977"
     CDS             6065..6979
                     /locus_tag="KPK67_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2458946.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            7021..8193
                     /locus_tag="KPK67_001978"
     CDS             7021..8193
                     /locus_tag="KPK67_001978"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBG2458947.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            8240..9052
                     /locus_tag="KPK67_001979"
     CDS             8240..9052
                     /locus_tag="KPK67_001979"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBG2458948.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            9256..10581
                     /locus_tag="KPK67_001980"
     CDS             9256..10581
                     /locus_tag="KPK67_001980"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBG2458949.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            10794..11873
                     /gene="mltG"
                     /locus_tag="KPK67_001981"
     CDS             10794..11873
                     /gene="mltG"
                     /locus_tag="KPK67_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBG2458950.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            12045..12719
                     /locus_tag="KPK67_001982"
     CDS             12045..12719
                     /locus_tag="KPK67_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBG2458951.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            12706..13953
                     /locus_tag="KPK67_001983"
     CDS             12706..13953
                     /locus_tag="KPK67_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG2458952.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            14025..15689
                     /locus_tag="KPK67_001984"
     CDS             14025..15689
                     /locus_tag="KPK67_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBG2458953.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYMLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(15786..16070)
                     /locus_tag="KPK67_001985"
     CDS             complement(15786..16070)
                     /locus_tag="KPK67_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2458954.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(16093..17610)
                     /locus_tag="KPK67_001986"
     CDS             complement(16093..17610)
                     /locus_tag="KPK67_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2458955.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
BASE COUNT         6931 a         1881 c         3162 g         5654 t
ORIGIN      
        1 cagtggttta ttttttaata taaatattga ctttgatgca ggtaaacttt gtatttttaa
       61 gcgtattatg gaatatgtta aataaaaaaa tgatgaaata tagtattgta aatgccaaag
      121 atgcaaaaca aacttaaaac atttatttta ttgttaagta atgctataat ataatgtgat
      181 tttaataatg atagtggagg tttaaatatg agagtcgagg cccctataaa agtagatcga
      241 aaaaccaaaa aacttgctaa aagagttgaa agtggggaaa tagcagttat aaatcatata
      301 gacatagatg aagttgctgc aaactcttta gtagaagcta aaataaaact tgtcataaat
      361 gcggctcctt ctataagtgg taggtatccc aataaaggtc cagggatatt aactgaaaat
      421 aatatactaa taattgataa tgttggagaa gagttatttg aagaattaaa agaaggagaa
      481 actatagaag ttgtagatgg aaagatatat agaaaaggaa aatttttagg tgcaggagaa
      541 gttttagaca agtatgaggt ttcacaccaa ataaaggctg cctatgagaa tttagctgta
      601 gaattggata gatttataga taatactata gattatgcta aaaaagaaaa aggatttata
      661 cttggagagg tagaaattcc aaaagtgaaa actaattatg ctaataaaca tgtattgata
      721 gttgtaagag gtcaggacta caaagaagat ttaagtacta tgctttctta tatagaagag
      781 gtaaagccag tattggttgg tgttgatgga ggagcagacg ctttaattga gtttggatat
      841 actccagatg tgattgtagg tgatatggat agtgtaagtg atgaagcttt aaaaaaggct
      901 agtgaaatag tagtacatgc ttatacagat ggaagagcac caggtcttaa aagggttgag
      961 gaattaggct tagatgcagt tgtatttcct gcgccaggaa ctagtgaaga tatagctatg
     1021 cttatcgcct atgagtataa ggctgagtta atagtagcag ttggtacaca ttcaaatatg
     1081 attgattttc ttgaaaaagg aagaaaaggt atggcaagta cttttttagt tagactaaaa
     1141 ataggttcaa agcttataga tgctaaaggt gttaatttgt tatacagaag taaattaaaa
     1201 ataaagtaca tatgggcatt aatagcaaca gcattatttc ctgtgttggt agttgcatca
     1261 ttgtcaccag gagttcaaca gttcatacaa ctaatgcaat taaagtttag agtattatta
     1321 cagatgtaaa ttttaggagg ataaatatgc atattaatat gaaatattat atagtcacta
     1381 taggggctat atttatagct ttgggtatag gcatgttagt aggatttaat ttaaataata
     1441 atcaacaatt aagtgaacaa caagcaaata taataaatga tttggatgat aagtttaata
     1501 tcttaaaaga aaaaaatgat aaacttgaca gtgacttagc aagtgtaaat agagattatg
     1561 aagaggcagt taattttata aataaaaatg tagataagat tttagctggt tcgctgaatg
     1621 gtaaaagtat aggaataata tctgctaatg aaaatgacga ttatacaaaa aatatagaag
     1681 acataataaa taaatctaat ggaagcattg catttaatat aatattaaaa gaaaacataa
     1741 caaatccaga aaagttaaag gaaatgtcaa ctaaactagg tacagatgta aaaaatgcaa
     1801 atgatgcagt taattatata attgacactc ttaaaaagga agatgcaagt gacattttaa
     1861 catatcttca agaactagat gtaataaagt ttaattttat tggagataca tatctaaaat
     1921 atgattccgt tgtaatagct ggtggaaatg atgcaaaaga cagtacaaaa cagtttgaaa
     1981 aaatagaaaa atttgtagtt tcaaaactta aatcagagaa taaatattta gtagaagttc
     2041 aaaatacagg agttaaaact tcttatgtag aactatattc taaaaacaaa gttgcaacta
     2101 tagacaatat tgatgaaggt ataggaagta tatctctagc gattcttcta caacaaggta
     2161 atatagttgg taattttggt agattggata ctgcaacttc attgttgcca tctattaaat
     2221 agaaataatt ttaaggagag aaaaaatgga ttcttatata agtataataa taccagctta
     2281 taatgaagaa agtagaataa aatatacatt agaaagcata ttggacatta agcaaataaa
     2341 tgaaataata gttgtggatg atggttcaat tgacaatact gctaaggttg tatctgagct
     2401 agaaaatgaa aagattaaat tttttaagtt agataaaaat agaggtaaag gatatgcttt
     2461 aaattatggt atcaaattag caatgaaaaa tgcagatatt ataggttttc tggatggaga
     2521 tttaggaagc tctgctaaag aagttgaaaa attaatagta cctatattaa atggtgaggc
     2581 ggatgttaca atagccaagt ttcctcctgc aaagagaaaa ggaggtttgg gatttgtaaa
     2641 aggtttagct aaaaattctg tgtttgagat gactggtgtt gagttagatg caactttgtc
     2701 aggtcaaaga atatttaaaa gagaagtttt agaaaagttt gatgaaatac cttttggata
     2761 tggagtagaa gttggaatga ccattgatat attaaagtat ggatttaaaa ttaaagaagt
     2821 attagtaaat atgactcata atgaaacagg aagaaattta aaaggtttta ttcatagagg
     2881 gaaacagtat tatcacataa aaaaggtttt aggacaaaaa aagaaagaat ggaggtaata
     2941 tcttagtgaa agattatata ctttatacca ttttattatt aactggatta tttggaacct
     3001 atgctgtaat tccactattt aaaaatttat tgataaatgg aaatgtactt agaccaaact
     3061 ataaaaaaga tatgatacca gtaagtatgg gaattgtatt tttgcctatg attgtaatca
     3121 atggaataat acttgctttc tttactacag aatttaagga tttagcgtat atatttatgt
     3181 ttatgtttgg catgatatcc atgttttttg cagggatact agatgatgtc attggaaata
     3241 gagatgtaag tggtttaaag gggcatttta aaagtttatt taaaggaagt cttacaacag
     3301 gaggttttaa agctttattt ggtggatttg tagggcttat agtttctgtt gctatatcaa
     3361 aagacataat tgatataata gtaaatacac taataatagc tctatcaact aatttaatga
     3421 atcttttgga tttaagacca ggaagggcaa taaaagcata tttattcata atggtaatta
     3481 tatttttaac attgacagga tttgttcaag ttcttccatt acttatagta ccaaatgtac
     3541 ttgcatactt taattatgat ttaaaagcaa gggctatgat gggagatact ggttcaaacg
     3601 tccttggaat atctattgga atgctcattt cttttgggta tccatttaac attagattag
     3661 gatggttaat atttgtagtt attatgcata tattcactga gaaatactca ttaactaaga
     3721 taatagaaaa aaataaacta ttaaatttta tagataaatt gggaaggtag tattatgata
     3781 agcaaaagag tggggattgt tgaatcaata gtaagtcaaa ctgaaactct agatgatata
     3841 agagtaaata taaatggaga aatacaaagg gcatataatt atcccaaaat atctggaact
     3901 ataaatatag gtgacgaagt ggttttaaat acaacagcag tagaactgag cttaggtaca
     3961 ggtggatacc attttgtaat aactaattta aataatatag aatctacttt aactgaaggt
     4021 ggacatataa tgaaacttag atatacgcct cttcaaataa aagtggattc tgttgaggaa
     4081 caagaaagta tttatcatga taaatttgcg aattttaaag gattagaagg tctttctgtt
     4141 gtagtaggga ctcttcatag tatgttaact ccatttgcag cttcatttaa aagaaataat
     4201 ccaaataaaa aattagtgta tataatgact gatggagcat ctcttccaat ttatttaagt
     4261 aaaaatgtgg atactttaaa agaaaaaaaa ttaatagata gtacaataac tataggaaat
     4321 gcgtttggtg gagattatga atgtataaat atatatactg cattaattac agcaaaagaa
     4381 atactaaaag cggatgcagt atttgtcagt atgggacctg gaattgctgg gacaggaaca
     4441 aaatatggat ttacagggat tgaacaaggc tctattcttg atgctgtaaa aaaacttgaa
     4501 ggaagagcaa ttgcaattcc aagaattagt tttgctgaca aacgagaaag acatcaaggt
     4561 atttcacatc atagtatgac tgtatttaaa gaaattgtaa atgtaaatgt agacatacca
     4621 ataactatat atgatgatga aaaattgaac tttataaaag agcaaatcaa tttaaatggt
     4681 ttagacaaaa agcataatat aatttatata gataataata aaacgaaaga ggatttagac
     4741 tattttaatc taaaagtaaa gagtatggga agaaattatg aacaagatga ggcatttttc
     4801 aaagctgcaa gcacagcagc atattactta atggaggttt aagatggtat tagaagaaaa
     4861 aactattagt agtgataggg tttatacagg taaagtaatt actttaaagg ttgatactgt
     4921 tgaaattcca gggcaaggat atcaaaaaag ggagttagta gaagttggtg gagcagtagg
     4981 aatagttgca atcacagatg ataataaagt tgtattagtt aagcaattta gaaaacctat
     5041 agaaaagcca atctttgaaa ttccagctgg taaactagaa aaaaatgaaa gtccaaaaga
     5101 atgtgctgaa agagaattaa aagaagaaac tgggtatagt gcaaaaaata ttaagctaat
     5161 acataaattt ttcacttcag caggtttttc taatgagata atgtttgtat atttagcaac
     5221 aggtcttact ccaggagaga ataatttaga tgcagatgag tttttagatg tctatgaaat
     5281 tgagcttgaa gaagcatata acatggtatt aaaaaatgac gttgaagatg caaaaacatc
     5341 tattggatta ttattagtaa aagatatgtt taaaaattaa tatttataaa atgaataacc
     5401 ctttgtccta aagcatatta ttttataaag gacaaggggg aattaaattg agaagaacat
     5461 atagaaaaaa tgattttaga gaattaaata gacaaataat tattatagga ttgttgttta
     5521 tgatgtctat tgttgttggg tcttacataa ataaaatatt gccaggttct agtaacaata
     5581 tattaaacaa tataaatcct gctgtagagt attataatct aaatataagc ataaaagata
     5641 cagtgataca aaatttaaaa tcagatgcaa tctttatggg ctcaatagct ctattaagtc
     5701 tatttgtagt aacaattcca gcagtgttgg tagcttttgt attaaaaggg atgtcaattg
     5761 gttatacaat aaatagttgt atattggcat taaaatttaa aagtataaaa atgattttaa
     5821 taatattatt taaaaatttg attattattc catgtgcaat catattagca ttaattagtt
     5881 taagttattt taaagaaatg gtttatgaat ttaaaaagaa aaatagaaaa aatatgcaat
     5941 ttttaattaa gaggtatata ctaaatatta taattataat agcactatca ttggggttgc
     6001 aattaatatt aaatactgca agtattggta ttattaagtt cctagctaaa taaaggagtt
     6061 tataatggat attatagagg gatatataga ttacataaaa gataaaaaaa aattatcaga
     6121 gaatacagtg gcatcatact ttatggatat aaaaaaatat atggaatata taaatcaaaa
     6181 aagcattaaa ctagtagata tagtagaaaa tgatattata agttacttaa taaagttgga
     6241 aaaagataat gtatcaatag ctacaatagc gaggatgata tcctctataa aatcttttca
     6301 tgattattta tttttaaatc atatatgtac aaataatcct gcaaaggata taaaaaaacc
     6361 taaaataaaa aaagaaaata taaatatact tacagaagaa gaaattgaaa gactattaaa
     6421 ttttccaaag ttaacaacgc ctaaattgat tagggacaag gctatttttg aagtattata
     6481 tggcactggt ataaaagtat ctgaattagt agagatgaat atagaggata ttgacctaga
     6541 tattgattat atatattgta attcatgttg taaaaatcaa agagtgatac cactttgtga
     6601 tattacaaaa ctttacttag aaaagtatct aaaagaagca agacctaaaa tggctgtaga
     6661 aggtgaaaaa tcattattcg taagttcatt aggtcaaagg tttacacgac aaggtctttg
     6721 gaaagtcata aaaaaatatt ctaatctagc caatatagat aaaaatataa atcctacaat
     6781 gcttagacat tcatttgcta ttcatttgtt aaatgaaggt gctaacattg ctgttgtaag
     6841 taaaatttta ggaaatgtta atttatctag tttgcaagta tatttaaatc atatagataa
     6901 aaacgtgaga agagaaataa aggagaaaca tcctagaaat gatgttgatg tagaattaca
     6961 aaaagcagaa gctaaataga ctatatagca aattttataa tttagaagga gcatatttat
     7021 atgagcagag taatttggat tgtaattgat agtgttggga taggagcatt acctgatgct
     7081 gaaaaatttg gtgatagtaa ggatgtaagt acacttggaa atatatttaa ggaatatcca
     7141 gatattcaga ttcctaatat gagaaattta ggtataggaa acatagatgg tatagatttt
     7201 tttgaaagta taaaagaacc aataggttgt tttggaaagt gtaaggaaat gtctcaggga
     7261 aaagatacta ctacaggtca ttgggaaatg actggtataa tagttgacaa accatttaag
     7321 acatttgaac atggattctc taaggagata atagaagagt ttgagaaaaa aactggaaga
     7381 aaagtagtag gaaataaacc agcttcagga actgtaataa tagatgagta tggagagcat
     7441 caaataaaaa ctggagatgt aatagtatat acatcagctg atagtgtatt tcagattgct
     7501 gctaatgaag aagtaattcc cctagaagaa ctctataata tgtgcaaaat cgctagggaa
     7561 ataatgatgg gggataatgc agtagctaga gtaattgcta gaccattcat aggtaagaaa
     7621 aaaggtgagt ttgtgaggac ttcaaataga agagattatt cactagaccc atttgaacca
     7681 acagttcttg ataatataaa agaatcagga cttgatgttt tagcagttgg aaaaatagaa
     7741 gatattttta atggaaaagg aattacagat gctatccaca ctaaaagcaa tatggatgga
     7801 gtggatgaaa ctttaaatta tatgaaacaa gataataaag gtctaatata ttcaaatctc
     7861 gttgattttg attctaaata tggtcatagg agagacccag aaggatataa gaaagctctt
     7921 gaagagtttg atagtagact tcctgaaata atggctaata tgagagaaga tgatatttta
     7981 ataatcaatg cagaccatgg aaatgaccca acatataaag gtacagacca tacaagagaa
     8041 tatatacctg ttatgattta tggcaataag attaaaaaag gttttaattt aggagtaaaa
     8101 gatacttttg ctgatattgg agcaactgtt gcagatatac taaatgtaaa gttgcctaaa
     8161 catgggtcaa gctttaaaga agatttattt taaactataa aatgataaaa acttagctta
     8221 ataggatgga gagattggaa tgtttgaaaa gatagcacaa agtagtaaat ttataaattc
     8281 taaaagtaat ataaaaccta agattggttt gatattaggt tctggactag gtgatttagc
     8341 aaatgatata gaagaccctg tcacaattaa atatagtgaa attccaaact tccctgtatc
     8401 aacagtagca ggacatgctg gacaacttgt aattggaaaa cttgaaggaa aagaagttat
     8461 tgctatgcaa ggtagatttc attactatga aggatattct cagaaagaag ctacttttcc
     8521 agtaagggtt atgaaagaat taggagtcga gattcttata gtaactaacg ctgctggagg
     8581 cgtaaataaa gaatttaaag caggagattt gatgcttata agagaccata taaattttag
     8641 tggaagtaat ccattagtag gaaaaaatga tgacagattt ggtgctagat ttccagatat
     8701 gtcagatgca tattcttcta aatatgtaga tgtagttaaa aactgcgcta aagaatgtaa
     8761 tatagatgtc aaagaaggcg tttatatgtt ttttagtggt ccaaactatg aaacaccagc
     8821 agaagtgaga atggcacaaa ttttgggagc tgatgctgta ggtatgtcta ctgttccaga
     8881 agtcatagta gcttctcact ctaatatagg agttattggt atatcttgta taacaaatat
     8941 ggctgctggc atcttaaatc aaccactaag tcatgaggaa gtaatagaaa ctactcaaaa
     9001 agttaaatca gaatttatga atttagttaa aacaacggtt aaaaatttat aagttaaagc
     9061 ttattataga ttttttgagg aagtttataa taaaatagtt aatgtttaat ttgaaaagaa
     9121 ttaataaaaa gctgtcttga tatagaaagt tatttctaat aaaggacagt ttttttataa
     9181 tactacaaca tttgaaagtg ttttaaagtt taatatttat ttaaattatt ataggttaga
     9241 atggagattt caattatgag aatgtatgat attataaaaa aaaagagaga taacctagag
     9301 cttagtaaaa aagagataaa ctactttata gaaaactatt caaagggaga tatacctgac
     9361 tatcaagctt ctgcactatt aatgtcgatt tatttaaata agatgaataa acaggaaact
     9421 gtacatctta cagaagcaat gatgaactct ggagacatga taaacctatc tgagatagat
     9481 ggaataaagg tagataagca tagtactggt ggtgttggag ataagactac catagctctt
     9541 atacctttgg ttgcttcatg tggagcacca gttgcaaaga tgtcaggtag aggtcttggt
     9601 catactggtg gtactattga taaattggag tctataccag gtttttcgac agaaatggag
     9661 ataagtaaat ttattgattc tgtaaataag tctaaaatag cagtttgtgg tcaaagtgca
     9721 aaggtagcaa ttgcagataa gaaaatatat gctcttagag atgttacagc aactgttgat
     9781 aatatatcat tgatatcatc aagtgtaatg tctaaaaagt tagcttcagg agctgatgca
     9841 attgtgctag atgtaaaaac tggtgatgga gcatttatga agacattaga agagtctttt
     9901 gagttagcta aatctatggt tgatataggt actggtatga atagagatac aatagggatt
     9961 gtaacagata tggatgaacc tttaggtttt gcagtgggaa actctttaga agttatagaa
    10021 gcaattgaga cattaaaagg aaatggacct aaagaccttg ttgagctatg cgaagtatta
    10081 ggagcatata tgttggtttt atcaaaggtg gcacatgatt ttgaagatgg tgttgaaaaa
    10141 ataagagaat caataaaatc tggtactgct atacaaaaac tcaaagagtt tatagagaat
    10201 caaggtggta ataaagatgt agtagatgat tattctttat tcaaaaaatc aaagtataaa
    10261 tatccagtta aatcaactaa atcaggttat ataagtaaaa ttaaagctga agatgttggt
    10321 gtatcggcta tgattcttgg agcaggtaga gaaactaaag atgatatttt agatttatca
    10381 gcagggataa ttttagaaaa gaaagtagga gattatgtaa atgaaggtga agttttagct
    10441 tacatgtatt ataatgatga acaaaagctt tcattggcaa agggaagatt tattgattcc
    10501 tactctattg tagattcaaa aatagaaaaa aataaattaa tatatggtat tgtaactaaa
    10561 gaagagatta aaaagtttta gaaaatttta gaaaagtgat tataaaaggc ctaacatgtc
    10621 aaaaataatg ttaggttttt tattttttga taaactataa attataccaa aaatatatat
    10681 tttaaaacat tttttacaat ataaataagt ttattctaca agatgattga aaattaaagt
    10741 atctagtttt ataatagaaa atggataaat atacataagg agtagattat attatgaact
    10801 ttaaagaaaa tagattgaaa atagcagtat taataattgt tattttgatt atcttagcag
    10861 gtatatttgt gtttatacaa ataggtcctt atgataaaaa taacaaaaaa gatgtaatta
    10921 tcgacgttcc aagtggagca tctgttggaa aaatatctga catattatat gaaaataaat
    10981 taataaagaa tgaactatta tttaaacttt tggtaaaggt tagcaataaa gctccgtcta
    11041 taaaatcagg aacatactta cttaatcaat cgtattcaaa caatgatata ataagtcttt
    11101 tagtttcagg taaaatatat caggatggta taaaagtgac tattccagag ggagctacat
    11161 ctaaagaaat tatagcaatg ttagtaagta aaaatttagg agataaagct acttttgaaa
    11221 atttaataaa aaaacctcaa gaattttatg ataaatttcc atatttgaag gaagatggaa
    11281 taacctcttt agaagggttc ttgtatccag aaacttatta ttttaattca aagaaacaat
    11341 cagaagaaga tattttaagc gaaatgttaa aagtatttga cagtaaatac acggacaagt
    11401 ttaaaaagaa acaaaaagaa ttaaatatga ctcttcaaga agtaatggaa atggcatcaa
    11461 taattgagaa agaagctgtt cttgacaaag acagacctat aatagcaagt gtgttttata
    11521 atagacttaa ggtaggtatg cctcttcaat cagatgctac aatacaatat atatttgaag
    11581 agagaaagaa aattgtaaca tatgatgatt taaaaattga ttcaccatat aatagttata
    11641 aaaataaggg attacctcct acaccaatat caaatcctgg tataaaatct attgaagcag
    11701 cattatatcc agagaaaaca gactatttat attttgtagc taagattgac ggtggaaata
    11761 attatagtac aaactatcaa gaccatttaa aatacgttaa agaatataaa gaagcaaggg
    11821 ataaacaatc aaaagataca aaagcaacaa ataaagaaaa tacaaaaaaa taaaccatga
    11881 ttataaaaag tctaacaggg taagaataat gttgggcttt tttattgaaa attgttattc
    11941 ttagatttat aataaagaaa gtgtaaatat atataacaat atgaatttaa aaaataataa
    12001 attaatataa aacttgaata tttattattt tagggagtgt ttttatgagt aatatagtta
    12061 atgatttggt tgaagattat ataagagtga cattaaaaga aaaagaagga tttttaaaag
    12121 atttagaaat ttatgcagag gaaaatagtg ttcctataat acataaagaa gtttcagatt
    12181 tattaaaagt cttgttaaag gttcaaaaac caaagagaat tttagaagta ggatgtgcaa
    12241 ttggttattc ttctattttt tttgcaacta ttataggaaa agatgcagat ataataactg
    12301 ttgagagaaa tgaaaaaatg atagaaaaag caaaagaaaa cataaaatta gctggatttg
    12361 acaataacat aactatacta gaaggagatg cagaagaaaa acttagtcaa gtccaaggtg
    12421 aatttgacct tatatttata gatgcagcca aaggtcaata taagctattt tttgatttag
    12481 ttatagataa attaaaagat gggggacttt tagtatctga taatattttg tataaaggta
    12541 tggttgctca tgatgatttt gttgtaagaa gaaaaaagac tatagttaaa agaatgagaa
    12601 attatttaga ttatatatgt aattgtgact atttaagcac atcattggta ccaataggtg
    12661 atggtgtagc actaagttat aaagaaagta aaagaggtga tgtagatgga attaaataaa
    12721 gtagaacttt tagctccagc aggtgatttg gagagattga aaatagctat aacatatgga
    12781 gcagatgccg tttatatagg tggagaaatt tttggaatga ggtctgcagc gaagaatttt
    12841 agtaaggaag atatggcaga gggagtagct tttgcacatg aaagaggcaa gaaagtattc
    12901 gtaacagtaa atataatacc tcataatgaa gattttctac aattagaaga ctatttgttg
    12961 gaactagaag agataggaat agatgcagtt ataatagctg acccaggagt gcttagtgtc
    13021 attaaaaaag taattccgaa tatggaaata catttaagta cacaagcaaa tacaaccaat
    13081 tatctaagtg caaacttctg gtacgagcat ggaataaaaa gagttgtagt ggcaagagaa
    13141 ttatcttttg atgaaatatc tgagataaga gcaaaaacac ctttagatat ggatatagaa
    13201 gcgtttatgc atggtgctat gtgtatttct tattctggca gatgtttaat aagcaactat
    13261 atgactggaa gagatgcaaa caaaggttct tgtgctcaat cttgtagatg gcaatatcat
    13321 ttggtggaag aaaaaagacc tggtgagtat tttccaatat acgaagatga aaggggtaca
    13381 ttcttcttta actcaaaaga tttatgtatg atagagtata taccagagtt aatcaaatct
    13441 ggtataacaa gtttaaaaat agaaggtaga atgaagactg catactatgt agctactgtt
    13501 gtaagagcat acagaatggc aatagatgaa ttttacagag acccagaaaa ctggaagttc
    13561 aatccaatgt ggatggaaga actaaaaaaa ggtagtcata gacattttac atcaggtttt
    13621 tacttgaata aaccaacaac agaagaccaa aattatcaat cagcatcata tgttagaaac
    13681 tatgatttta taggtatagt tagagaaaca gaagatgaag atggtcttat tgttgttgag
    13741 caaagaaata aaatgtgtgt tggtgatgaa atagaagtta tgggaccata taaagaaact
    13801 atgtttacta aaattgaaga aatgtacaat gaagaaggag aagctataga atcagctcct
    13861 catcctagac aaatagtgaa gttaaagcta tcagtaaaag ttggtaaaga ttatatgctt
    13921 agaaaagtta tagaagaaaa ggttgaagaa taataaaaaa ttgataaaaa tagtattagc
    13981 cccttagaaa ttgtcaataa tgaaattatc taaggggtga tttcatgtca aaaaagaaga
    14041 ctccattttt gaaaaaagtg ggaaagagaa gctggtgtat atttactata attttaataa
    14101 tttatagtgt tttaatttat atgctagttg atatacaagt attaaaaggt gataaataca
    14161 aacaaagtgt tgaatcacaa agcgttgaga aagttgagtt aaatagtgga agaggaataa
    14221 tctatgatag aaacaacaag aagcttacag atacaagtaa atctcaggta ttaattgttg
    14281 aaaaagaaaa attaaataat aactataaaa ttttggagct tattaagaaa gctactaaga
    14341 tgaatgattt agatatatat aaagctgtac aagagcaatt gacacgccct ataatacaga
    14401 ttcagactaa aaatatcgat aagagtatga aaaaagaact tgagaaaaat ggaataatgg
    14461 tagaagaaaa aactatgagg tacgcaaaag atggtttatt atcacataca ataggatata
    14521 taaaagaaga tgataaatca ggtcaatcag gaattgaaaa gagtatggat agtgtactta
    14581 gaaattcaaa tgaaaagtat ataagtgctt ttaaagctgg agatgcagga aatgaaaaat
    14641 ctctaaacat actaaaggga agtgtaaaaa ctgttgataa taaggataaa gatagacatc
    14701 taaaaactac tattgattat aacattcaaa aaaaattaga acaaatacta aataaagaag
    14761 aaaatcctac agcagcaata atctcggaag catctacagg tgaaattctt gcaatgtgct
    14821 ctagacctaa ttttgaccaa aatgacatat caaaaagttt aaaaggaaaa aatggggaat
    14881 ttgaaaatag agttataaaa gcgacatatc caccagggtc tgtatttaag atggtagttt
    14941 tattttctgc acttgaaaat ggtgttatag atgagaacta tacttacaac tgtacaggta
    15001 agactaaagt tgggaatact aatgaaatac tgcgatgtaa taaaagagat ggtcatggtt
    15061 tccaaaactt aagacaagct ttttctaatt catgcaatcc tgcattttta gatatagcta
    15121 tgaaacttgg aaaagaaaag atactcaaat ctgctgaaaa attacattta tttgaaaagg
    15181 ttgatattgg tcttgatgaa gaaaaaatta gagaagctcc aaaaaacata tcaataagaa
    15241 atttagctat aggtcaagaa aatatagaat ttacaccact tcaaataaat caaatgactc
    15301 aaataatagc taataatgga acattcaaac cactatactt atataaaagt cttgtggaca
    15361 ataatatgaa tacaataaaa acttataagt cttcaaaaaa agaagaactt atttctccct
    15421 atgtgtgtac acaagttaaa gaatatatga agtctgtatc taggattggt acagctaaag
    15481 acctaaaaga tattgaaggt ggttgtggag ttaaaactgg tacagctcaa agcagtttaa
    15541 ataaaaaagc tatagaccac ggatggataa caggatttta tcctgaagaa agacctaagt
    15601 atgtaataac tgtgttagta gaaggaactc aaaaaggaaa taaatctgca acacctatat
    15661 ttaaggaaat atgtgaaagt ataaaataaa ttatgtacta taaaatttaa ggatatatcg
    15721 taactttgat tttagataga aaattttgtc taatgatgta cgatatatcc ttttaaaata
    15781 tagtattaac tttcttgaac aagctctttt tctagttttt taagagcttt tttttcgatt
    15841 ctagatacat atgaccttga tatatcgagt ttttgtgcaa tttctctttg tgtcttatag
    15901 ccataaggag ttaacccata tcttaactgg acaatttctc tttctctagg agtaagaatt
    15961 ttgtttagtt gttcgtaaag ttttccaact tgaactttta gttcaacttc atcaagaaca
    16021 tagttagcct ctgtaccaag tatatctaaa agacttattt cattacacac tactctttat
    16081 atgttattgg tattactggt cttcatgtaa tttcaaatgg accattaatt caaaagaatc
    16141 tagtttttgt tctttagact tatagtaaac tgctttatca attacagatt ttaggagttt
    16201 attttttcca agggaatctg aatcatggta aagtgataac acagttttaa tatcttctat
    16261 tattttagaa ttgtcttttt taacagtact caatctattt tttactgtct taattgaatc
    16321 tttaatttct tcagttcttg aacttatatt gttggacctt tcaataaatg tatcttcatc
    16381 atatattcct cgttctagta aatcatataa cttatttttt tgcttgccta attcttttaa
    16441 ttcagtttct aaacccttta agactcgttt ttgaaattct atattactat ctttattatt
    16501 tttatctaag tctatatcag atgcttttaa ttgatatttt tttacagtgt cttccaaaga
    16561 ttttaaaatg gcagcttcaa taaattcaaa tcgagaactt ttattgcagc caggatgata
    16621 acaaattata tagtcatggt ctgcatatgg tctatatact aatggtctac cacaattttt
    16681 gcaaatcact attcctgcaa gtggattttt tatttgatta ccatagctga catgaccatt
    16741 ttttttgagt atattttgag cttttttcca tgttatttca cttattaaag gttcatgctt
    16801 tccttttgct tcaatccatt catctttagg tcttagttta actgttttta ttttatttgg
    16861 atttttagat tttttgtagt cctttttttg ccactgaatg tagccacagt aaaccttatt
    16921 ttttattata tttgtaattg cagaattgta ccaattagca cctgtagcag ttttaatgcc
    16981 taagttattt aggtattttg atattttact acaacccata tcttcattaa tatataagtc
    17041 aaagatagtc ctaactacat cagcatcctt atttggtaca agtattcttt cttttctacc
    17101 tgttgttaca gcatcatatc caaatggagc agaagttcct atgaaattcc cttcttcaac
    17161 tgattttatt ctacctcttt gcattcttct tgatataagt tttaattctt ttcttgccat
    17221 aaaggcttca aattctgagt actcctcatc aaattcatct gttaggtcgt atgtttttct
    17281 aggtgtgatg attttggtct tactttcttt gaatgtttct aatatgatac cttggtcttt
    17341 catgtctcca cgaccaagtc tgtctaagtc cattactaag actgcatcat atttattttc
    17401 ttcaacttct tttagaagtt caagcatttt gggtctatgt attatacttt caccagaaac
    17461 taattcttct tttatttcaa ttacatctaa gttttgttct tttgctagtt ttagtagggt
    17521 tgatttatgt ctacttaaag tttcaaattc accttgcttt tctgcttctt catcagcacg
    17581 agattttctt aagtatatgg caacactcat aataagtaat cacactcc
//
LOCUS       DAEMHW010000071        17380 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEMHW010000071 DAEMHW010000000
VERSION     DAEMHW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.071"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..217
                     /locus_tag="KPK67_001987"
     CDS             <1..217
                     /locus_tag="KPK67_001987"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458956.1"
                     /translation="IFIFRFLGGLVVFKDKIDECVHIMTAYIASLKEYYSFIETQIGD
                     FIKKYGEDVVELCLHRVMILLCECGLA"
     gene            1049..1963
                     /locus_tag="KPK67_001988"
     CDS             1049..1963
                     /locus_tag="KPK67_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBG2458957.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            2353..3000
                     /locus_tag="KPK67_001989"
     CDS             2353..3000
                     /locus_tag="KPK67_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG2458958.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(3167..3736)
                     /locus_tag="KPK67_001990"
     CDS             complement(3167..3736)
                     /locus_tag="KPK67_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458959.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(3741..4640)
                     /locus_tag="KPK67_001991"
     CDS             complement(3741..4640)
                     /locus_tag="KPK67_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2458960.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYEDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(4651..4800)
                     /locus_tag="KPK67_001992"
     CDS             complement(4651..4800)
                     /locus_tag="KPK67_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458961.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(4793..5377)
                     /locus_tag="KPK67_001993"
     CDS             complement(4793..5377)
                     /locus_tag="KPK67_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG2458962.1"
                     /translation="MKRFFIVVLGCICVLGVISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            5798..7123
                     /gene="cdeA"
                     /locus_tag="KPK67_001994"
     CDS             5798..7123
                     /gene="cdeA"
                     /locus_tag="KPK67_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBG2458963.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILYTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIFALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFALGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKGRYKY"
     gene            complement(7218..8012)
                     /locus_tag="KPK67_001995"
     CDS             complement(7218..8012)
                     /locus_tag="KPK67_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2458964.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(8013..8888)
                     /locus_tag="KPK67_001996"
     CDS             complement(8013..8888)
                     /locus_tag="KPK67_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2458965.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(8892..9260)
                     /locus_tag="KPK67_001997"
     CDS             complement(8892..9260)
                     /locus_tag="KPK67_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2458966.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(9443..10102)
                     /gene="deoC"
                     /locus_tag="KPK67_001998"
     CDS             complement(9443..10102)
                     /gene="deoC"
                     /locus_tag="KPK67_001998"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBG2458967.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(10138..11682)
                     /gene="murJ"
                     /locus_tag="KPK67_001999"
     CDS             complement(10138..11682)
                     /gene="murJ"
                     /locus_tag="KPK67_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG2458968.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILITGIIFIGITSVMSAFLQIKENFIVVGF
                     GSIPYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKD
                     DSLIKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTA
                     SIVSVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFER
                     GAFDARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVL
                     DLVLIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIM
                     GVLSYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKR
                     KLHLKK"
     gene            complement(12156..13040)
                     /locus_tag="KPK67_002000"
     CDS             complement(12156..13040)
                     /locus_tag="KPK67_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2458969.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(13331..13807)
                     /locus_tag="KPK67_002001"
     CDS             complement(13331..13807)
                     /locus_tag="KPK67_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBG2458970.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            14074..15249
                     /locus_tag="KPK67_002002"
     CDS             14074..15249
                     /locus_tag="KPK67_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBG2458971.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(15330..16133)
                     /gene="proC"
                     /locus_tag="KPK67_002003"
     CDS             complement(15330..16133)
                     /gene="proC"
                     /locus_tag="KPK67_002003"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG2458972.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(16173..16757)
                     /locus_tag="KPK67_002004"
     CDS             complement(16173..16757)
                     /locus_tag="KPK67_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2458973.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         6003 a         2748 c         1934 g         6695 t
ORIGIN      
        1 tatttttatt tttcgttttt tagggggatt agttgttttt aaagataaga ttgatgaatg
       61 tgttcatata atgactgcat atattgctag tttaaaagaa tactattcat tcatagaaac
      121 tcaaattggt gattttatta aaaaatatgg agaagatgtt gtagagttat gtctccatag
      181 agtgatgatt ctactatgcg aatgtggttt ggcataaaat taaatgtatt aaaatgggta
      241 agaagaatcc tatataatat tcttcttacc cattttaata tatcaatttc atagcttatc
      301 gtttaaaata ttttacatcc attactagac aactttacat tctcttttta attctagtat
      361 atttaaatca ttttagttgc tccttattta ttcttattac ttgttttatt ttataaataa
      421 aataattctt tttaataatg ttttttgttg atattcctat cacacatcca attatttatc
      481 attgtcttga aattgtttgc atacttaatt taatccaatt gaaacacccc agcatagaat
      541 cccctgtaaa atagaagttg gattaaatcc taacatctat acagaaaata ctactccaag
      601 cacaagcaat ataatggaat atatttatca cttaattttt tatacttttt aaatcccata
      661 cctaaaacat taagtgcagc tattagaatt aataattgtt gtggtataaa attatgcttg
      721 cttcatattt attcattttt tgctataaaa gttcttgttt tacaaaaaac atttgttttg
      781 tatcaaaata atacttataa attacataat tctcataaaa atttctagta ttacttgtac
      841 tcttaataaa taatagccaa caagtacctt aaatcattat atctatatct aataagaaac
      901 acagtaagat aattttttcg cccataactt aataaaaact gttgatatta gtagcaattg
      961 aatttagtta atttatttat aatatacata aatttaaata ttacgaataa agtattataa
     1021 ataggatact ttctaaaggg aggaaattat gatagataat caaaaatatg ttattttatc
     1081 actagaatta catttatttt tttcaagaat tatgaaagag catgctcttt ttttagaagc
     1141 aggattcaca aataaaaatt ataatcttgc tatggaagct gaccactata aaaagcaatt
     1201 tgaagattta ttatcataca ctgttagtgc tagtaatggt ataattagac ctgatatatt
     1261 atattcagaa gaacttgtaa ctactctcac atcagttgca gaacaaaaaa cagaagagtt
     1321 tacagggata gaaataaaca aaaacatcac tacaagagaa ttaaatctac aaagtggtgt
     1381 aaacccacaa gttggtcaag acttagtgaa ctatgtagcc caacttaact ctgatgcaat
     1441 aagattactt gatgggctta ttaattttaa agaaagagtc ttagatggtg tactatcatg
     1501 tactatattt acatcaaact accctctact tcttgaacat ataatacatg aagcaaattt
     1561 atatcgttct tatgtagttg accttgaaaa taaaatagat attgagtcaa aaaacgctaa
     1621 agaaatagaa ttattctggg accatattat gatggaacat gctctgttta tgagaggatt
     1681 actagacccc tcagaaggtg aactaataaa tacttcaaat gattttgcta taaaatttaa
     1741 tgaattaatt gaaaaaacaa acgaaatgac tgattctaat atcaagaaca ttacagaaga
     1801 aactctaaat gaaactgttg agtttaaaga ttttaaagaa gcaggagcat caggaataga
     1861 acagtgtaag ataaaatcta taatattacc acttttagca gaccatgttt taagagaggc
     1921 aaatcattat attagaatat tggagagtta taaaaacatg taattaaaaa ttacttttaa
     1981 aataatcttt gcatatttag ataaaataaa ggtacagtta actgtacctt tattttttat
     2041 aaacttataa aatttaaaca agttattcat aaaataaaat aataatcatc tataacccac
     2101 aactttattt tatattaaat aaaaagtatt agtgattatg ttttataagt atttctttat
     2161 tcagttattt gttaaaaact aatagtaaat cttatactaa tcgcatattt atgttataac
     2221 tatattgtaa ctattttcgt aatcttagga aaagattact tataaaatta accttataca
     2281 ataatatatc gtataatctt aattagcata atagttacaa tattaaaatt taaatatggg
     2341 agtgatttta gtatgataag acaaacacca ttagagaaac gatatgacaa gctaggtcct
     2401 catattgtaa cagcactcaa aaaacgttat tttgatgctt attactgtaa aactagaaat
     2461 gaagctttgc aacaaatttt agatttaatt cctcaaaatc atgtagtatc atggggagga
     2521 tctgaaacac ttaaagaaat gggcgtacaa actgctgtta gagagaaagg atttaaagtt
     2581 attgatagag atttagcaaa aactccagaa gaaagaactg aaattatgcg tcaagcactt
     2641 ctatgcgata cttttttgat gagtagcaat gcaattagtg aagatggaca actctttaat
     2701 attgatggaa atggcaatcg tgtagctgca atgatatttg gtccaaaaaa tgttatagtt
     2761 gtagctggta tgaataaaat tgtaaaaaac attgatgatg caatacaaag aactagaaca
     2821 attgcatctc cagcagttgt tcagcgtttt caagatgtca atacaccatg ctatattaat
     2881 ggaagttgta tagattgcac aagccctgaa agcatttgtg catacatggt tactacaaga
     2941 atcagtcgtc cagcaaaaaa aataaaggta atattggtag gagaaaatct tggtctataa
     3001 aatatacata catgtaaata gctctataaa taataaaatc atatgaaata acttatttct
     3061 aaaagtggta gaataaaagc ttctacaaga ctttcattct gccactttta tattctttta
     3121 atttgtgttt ctatattatt aattgctcca attttcatat atattactaa ctatttttat
     3181 tttcataatt tttttgttct tcatcaagct gtttataata atctttatta tagtcagcta
     3241 gataatctat accacctcct gtaatcacat ttaataattt cattgcagat tcttgattaa
     3301 ctttacaaac ataattatca ccagatttaa caagctctaa tgtaatatta aattcttttt
     3361 tctttgcaga atcaatatct ttcatagttg gatttggcat catttctcct agtttaggag
     3421 gattcttttt taaaatagaa accatatcag tattgttaat ttttaaatct acagtcactt
     3481 tatccttatt attctttata tctgaaattt cccaagtcat atttttagaa tacattttaa
     3541 atccttcttt atcttcgtat ataagattac ctaaccctga ttttgggaaa tacttatcaa
     3601 cagtatctgc atcttgtgtt tttattgaat taaacatact atccacttca ttttttatct
     3661 tattatcatt agtacaacca gctaacacca gtactgatat tgcaaacatt ataatacata
     3721 gaataaattt cttcattaaa ttatatcccc ctatactttt ttgcacaaat attattctct
     3781 tttttatctt ttgaaaaagt tgtagtaatt gcattttgtg gacaaacttt tatacattca
     3841 ccacacctaa tacagtctgg actatttaca tcttcatata ttggtatatc aagcttacat
     3901 tttgtttgac atgctgtaca atttgtacat ttatctttat caattttaaa tctatatatt
     3961 gaaataggat taaaaaaacc atagattgct cctaaagggc atatatactt gcaaaatggc
     4021 ctataagtaa tgattgacat caataatatc aatagcaaaa tacttaactt ccagctaaat
     4081 aaaaatccta ttgattctct taaattctca tttcctaaaa taagtggtat tcctcccaaa
     4141 agtgttcctg aagggcaaat ccatttacaa aaccaaggat ttccaccccc tgctacattt
     4201 acaatagcaa ctggcaaaat aattacaaac attattaaga ctatatactt tagatatttt
     4261 agatatttat caccataaac tttttttatc tttactggaa agggtacttt atgtagtaaa
     4321 tcttgtacaa gtccaaaggg acaaagccaa ccacaaacaa atctgccaaa aaaagcacca
     4381 aatgccatta aaaaaccaac aacataaaat gagaatttat actctctgct tgataaaact
     4441 gcttgtaatg aacctattgg acatgctcca agtgcacctg gacatgaata acaattcaat
     4501 ccaggtacac ataatttttt actttctcct ccatatatag ttccttctaa aaatccattt
     4561 acatatccat ttgttaatgc tgtaaatgca acttgagtaa taagtcttat attattattt
     4621 accttagata ataacttcat tcctcttctc ctaaccaata ccaatacact caagacaaat
     4681 cataattgct ttagttaata ctgtatttgt ttctccacga tatgtaccta atgcaataaa
     4741 tataatacta attgcaataa tagaaatgcc ttttttatta tctttaataa aattactcat
     4801 tggcttttac catttttagt ctttcatcta taattttttt ccaatcttcc ttactatttg
     4861 cacctacaac cttctcaccc acaaagtttc ctttcttgtc tacaaaataa gttgttggaa
     4921 actccataac tcttttaaaa tcagtttttt taagttcttc agatacagtc aattgttggt
     4981 aagttgcatc tgactttttc ataatgtctt taactaaatt tttctcttca tttgaaagac
     5041 cagttctcat atcagtttta tcaacttcta caactaatcc ctttatagct acattggaat
     5101 tattactctc atattctttt gataatgctt caagctctgg catttcaccc acacatggtc
     5161 cacaccaagt agtccagaca ttaataagag taagttcttt atttgaaaat atctcttttg
     5221 ttaccttatt tccatcaaca tcctccgtat ctaaattact aaaccattca ctattattct
     5281 tgtttttttc tactggctta gattcttttt tgctacatcc agaaatcaca cctaaaacac
     5341 aaatacatcc taaaactact atgaaaaacc ttttcatatc tcacactcct agaatttata
     5401 tagtatataa aatcgctgtt tgaaataaag ataacttctc ttctatatta aaacattata
     5461 tttaataaat aagaactatt taaaataagt tgtataaaag atatacagtt aaaatctatt
     5521 tcttttcaga caaaatctta tatatatata ataacttata aaacttaaga ttatatacat
     5581 atatttctaa aaattttctt aagaaacaat tataccttac taatgcaata tacctatttt
     5641 tactaatata agtttattaa ttagcactat actaatttat cttaatatga ttttttgttg
     5701 attttaagct gtttatcgta taaataaaat ttttcttgac tatatagtta ctatatagct
     5761 tattatatat agagagtaat aaaaggaggt aaaatttatg gaaaatttat ttacaagaaa
     5821 attcactact tttgaatttc taaaatttgt ttctcctgca attatatcca tgatatttat
     5881 atctttgtac acaataatag atggcatctt tgtatcgaca ttagttggtt ctgatgctct
     5941 tgctagtata aatattgtac tacctataat taaccttgtt tgtggatttg gaataatgat
     6001 ggcaactggt ggaggtgcta tcgtttctat acgtatgggt gaaaatagac aggatgaagc
     6061 caactctaca ttttctttta tagttttgtt ttcattgatt gttgggattt tattcacagt
     6121 aatctcatat ttctttatca aagaaatatc tattttgctt ggtgcaacag ataagttatt
     6181 accatattgt ataacttatg gtaaagttat gattttatat acaccatttt atattttaaa
     6241 atttatattt gagtactttg caagaactga tggaaattct aaatttagtt tatttctatc
     6301 agtcattggt ggtgtaacaa atataatatt ggattatgta tttattaaat attttggaat
     6361 gggtctttta ggagctgcag ttgcaactgc tataggtatt attttaactt gtgttttagg
     6421 tattatttac ttcttatcta ataaatctac actaaaatta agaaaaccaa aaaccgattt
     6481 tagacttata agagatacta tgataaacgg ttcttctgaa atggttacag aattatctac
     6541 aggaattaca acattcttat ttaatgtagt agctttaaaa ttagcaggag aaaatggact
     6601 tgctgctctt actatagtat tgtatgctca ttttttaatg acatcagtct atctaggatt
     6661 cgctgctgga gtgtctccat taataagcta taattttggt gctgaaaaca gtgataaatt
     6721 aaaagaaaca tttaaacatt ctctaaaatt tatatttgtt tcttctcttt tagtgtttat
     6781 ttttgcttta gtatttgcac catttattgt tagggtcttt gtaagtccag ataacacagt
     6841 atttaaacta gccttacaag gattaaaaat atttgcattt gcttttttgt ttgttggtat
     6901 aaatatattt gcattaggat tttttacagc atttcacaat ggaaaaattt cagctattat
     6961 atcttttagt cgtgcctttg tttttataat cataggaatc ataattcttc ctcctatgtt
     7021 aaacatgact ggattatggc ttacagttcc atttgctgag gttataacca tatttatatc
     7081 tattctattt ataaaaaaat ataaaggtag atataagtat taaaaattaa tacttaaaat
     7141 caacatttat taaaactaat aaacctgctt taaaatacaa atttacaaat ttgattttta
     7201 agcaggtttt attttagtta taataagtca tttgcatatt ctttaattgg tgcttttcta
     7261 agtaataaaa ttataaatat tatgcaaata actataattc ctacttttgt tccaaataaa
     7321 ataaaaggat tctgaaatga ataaataatt ttttctaaat acaatagcat tttatgcata
     7381 tttttagttg caaaattaac tatataaatt accattaata tcggtaatcc tatcaatata
     7441 gtaatactag taacttttct aactctatat gtaaaagctc caagtaatac tcctataaaa
     7501 cctatacata tttcatttat acatatttgt acaaaataat ttaaataagt tagtggcata
     7561 cttgatgttg aaggatctat cagtgctgat atagatgtcc aaatcatatc tctctttagt
     7621 aaaactgata gttctatact gtaacctgtt ataatttcta taaataactt cgataaaaaa
     7681 atcaaaacta tacctaaaat agaaatcaca aagcttaaaa taattaaatc tatgacaata
     7741 gcttttatac aactttttct atctgctctt atacttaaag ctcctgaaaa gtctttattt
     7801 gctatattta ctccatacac aaatagcagt atagttatat atgttaaaat aggattcaca
     7861 atcgtggata aattagtttt agatgcctct ccaaaaatct tgaggataaa cactggcaaa
     7921 atattcataa caattcccac aatcataaat ataattatat acaatttagt atttttatta
     7981 aaaaatttta tatatggctt taattcattc aattacaatt cctccttctt agtcatgtta
     8041 acaaacatat cttgtagccc tatgtatcct acatcaatat ttgaattttt aagtattttt
     8101 tcatcatttt cactaaaatc accatagtaa aaataagcaa ctgcatttcc aaatgccttt
     8161 ttaggtttta tattttttat acattctaat ttttctaact cttctttatt tccagtaata
     8221 taatgagctt tttgttttat gacatcaatt tcttcatcta taataatttt tccctcattt
     8281 aatattatta catgctccag taaatcatct atttcatcaa tcaaatgagt tgatattatt
     8341 attgtccttg gattttttat ataactatct aaaattatgt tataaaactc ttgtctattt
     8401 actgcatcca aaccaattgt tggctcatca aatatggtga tagctgaatt agaacaaata
     8461 cctattatat ttgaaagtat agttttcata cctcttgaca gttgtttata ctttttttta
     8521 gtatttaaat caaaatattt gcataattct tcttctaagc ctttatcata acttttataa
     8581 aaactagaag aaaattcaaa tatctgacct actttaaaat ctggactata aaattctttt
     8641 tccctgacaa tacaaacttc ttccaaaact tttatatctt cttttgggtt ctttcctaat
     8701 atttctatct caccttcatt ttgaattaac tgatttacca ttatatttaa taatgttgtt
     8761 tttccagctc cattcttacc taataaacca tatatcttat ttttttcaaa gtttagagac
     8821 atattattaa aaatattttg cttcttaaaa ctgtgagata aattttctat cttaatagaa
     8881 ctactcatat attaatcctc cttgaaatta attataatat caaccaattc ttctctattt
     8941 atttctaatt tgttagcttc ttgaagtagg ttttttacaa aattatgttt aaaattttct
     9001 tttctagctt cttttataat agtttttgca ccatctgata caaacatccc tatccccctc
     9061 ttcttatata aaatattttt atcaaccaat atatttatac cttttaatac tgtagctgga
     9121 ttaattttat aaagctttga aagctctgtt gttgatggaa cctgttcttc ttcttttatt
     9181 ccatttgata gtatctcatc ttcaattgct cgtgctatct gaataaatat aggttcttca
     9241 ttatttaata ctaatttcat aggttcctcc tttctccatt agttagttac tcaactaatt
     9301 aactatataa cttataatat cttgaaaaat attttctgtc aatactaaat aaaaaacaga
     9361 gggaaaattc cctctgtttt ttgttcttta tattcataat taaattttaa tttttgtaat
     9421 cctatcccat aagttaaaaa ctttaataat ctgttgtatc ttcatttttt aatattttac
     9481 atattgagct agttcctaac ctttctgctc ctgcctctat aaatttttct gcatcagaaa
     9541 ttgatttaac tccccctgca gcttttattt ttacattttt tccaacatgc tctttcataa
     9601 gctttatatc ttctaaagta gctccacctg ttcccattcc agtagatgtt tttataaaat
     9661 ctgaacctga cattgttact atttcacaca tttttatttt ttcatcttca tctaaataac
     9721 atgtctctat aatgactttt aatactttat ctcctatagc tttttttatt tcttttattt
     9781 cttttcctat attatcataa tctttatttt ttatatctga aatgtttaca accatatcaa
     9841 cttcatctgc tccattttca atagcatctt ttgcttcaaa tacttttgta gcagtagttt
     9901 gatatcccaa agggaaccct atcactgtac atatctttat tttacctttt agatattctg
     9961 atgctctttt cacatatgat ggtggtatgc aaactgatgc tacactcata tctacagctt
    10021 catcacataa aattttaata tcttcccatg tagttgtagc ttttaaaata gtatggtcta
    10081 ctgtttttaa tatatgtttc ataaaatcct cctaaattta taattaatat aattagatta
    10141 cttttttaaa tgcaattttc tctttgcaat attcagtatc atatcaacct cttcaacttt
    10201 aaaaatagtc attagtagtg tataaattcc acctccaact ataactgaaa tagctagtga
    10261 tacaaactca ttaaatgttc ctactcccaa aattccaaat ataaatttat acgtaaagta
    10321 tgataaaaca cccataatta aagatgcaac taccgatttc aaagttgctt ttattatttt
    10381 atcttgtcca aaatatccaa cttttctctt taggttcaaa aagagtaaca atatacatgc
    10441 aattgatgat gaactagttg caagagcaag accaccatga gccattggtt ttattaaaac
    10501 taagtccaat actatattta cccctacaga tataatccca tttaccatag gtgtttttgt
    10561 gtcttgtaat gaataaaaaa cctttcccaa aatatctctt aagccaaatg cagtcatacc
    10621 aactgcataa aaaattaagg cagttgctgt catttgggtt gccctcgcat caaaagcacc
    10681 tcgctcaaaa atgattctaa caacaggagt tgcaaaaaaa atagaagcaa ctgaaatagg
    10741 aatcatagaa attattatca tatttatact agatttaact gaactagtaa acttcttttg
    10801 attattttct gcacttaact tagataacat aggatacata acagatacta ttgaagctgt
    10861 aaatgtccct gtaacaaaac catttaatct atctgcataa gtaagtgctg gaatgcttcc
    10921 tttaactaat gttgaggcta aagttgtatc taccaatgag ttcacttgat taacagcaac
    10981 acctataaat actggtgaaa gtaatgctag taatttaata aggctatcat ctttaaagtt
    11041 taagtagtat aagtatttgt agttcgtttt tttaacaaaa aacatgtaaa agagcagttg
    11101 aactaccata gcaaccacag caccaattgg aagtatgtat ggtccaaata tggtacttaa
    11161 cattattgaa ataattatta ctatattata gggtatactt ccaaatccaa ctacaataaa
    11221 gttctcttta atttgtaaaa atgctgacat aacactcgta atacctatga atattattcc
    11281 tgttatcaat attttagtaa attttacggt aagtaaaaac ctctctcctt caaatcctat
    11341 agcaaatata cttacaagtt gctttgagaa aataactcct aatatagcta caactatgca
    11401 tataccaact attatattta aaacattgtt taaaaacttt agtgcttgct tttcaccttg
    11461 tttactactt atatcttgat acattggaat aaaagtagta actatagcag tacctattgc
    11521 tgcaaatata atatttggta tattcatagc tgttaaataa ctctctgtat ataagcctgt
    11581 accataaatt gatgataata ctaattctct acccattcca agaatcttag agattatagt
    11641 aactatcatc aaataaaaag ttgctttagc taccttgctc attttatcac tccacttcta
    11701 acatacctct ttttaatcaa gtaaaattta gctcattaga tataattcta tcaactaatt
    11761 tgccttgctt agccctatca aaatataatt aacacctatt tcttaatata tcataaacta
    11821 cattattctt catatttttt tatttctcca ataagtttta catctttcct atataaattg
    11881 tatagcttca attatattca aagaaatgga gttgctaccc gataacttta aaagtaaaaa
    11941 acagatttta ttatcttttt aaactatatt ctaacatatt ttgtccatat tgagaaattt
    12001 tactaaacta ttatgtactt tattaaataa ataaaataac agcacaccta taaaatacaa
    12061 gtgtgctgtt cataaacaga ttttataaaa aaataattcc taagtagaga ttaacatgct
    12121 ttctcatact ctcatatata atgtgtctaa aaatattaat aaaaatgctt taattgtttt
    12181 aacttacttg gatgtcttaa ttttcttatt gctttagctt ctatctgtct tattcgttct
    12241 ctggtaactc caaatacctt tccaatctgt tctagtgttt tagggtcatc atcatcaatt
    12301 ccaaatctca ttcttaaaac ttgctgttct tgctcaccaa gacctgtaag tagttcttct
    12361 atcttttctt ttaaattatg ctgttcaact tcgtgtatta caacatcttt aaaatcagca
    12421 tctgatggta taaattcaac taaactacta tcctcatctt cttttaatgg agtatccaaa
    12481 gatacaacat ttttatctct tcttaataat tccaacactt tgtcaacttc taagccacat
    12541 gcatctgcaa tctcatctat agttggctct cttaataata catttaaaag ttcttgtttc
    12601 tttttcttta caaaatttat tctctgatga agatgtatag gtatcctaat agtattttcg
    12661 caatcgtcga tatatcttgt tatactttgt tttatccacc aagttgcata agtgctaaat
    12721 ttgtatcctt ttttataatc gtacttttct acagctttta taagtcctat atttcctgct
    12781 tgaattaaat ccaacatatc aatactatct cttttatatc tctttgctat gctcactacc
    12841 aacctataat ttgaatttat aaatttttct tttgctactg aagatgagtt gattatttcc
    12901 ttagccagct ctacctcttc tcctgctgac aacattttgt attcctctat ttcctttaaa
    12961 tacattttca tcgcatttga tacatttgat gaacccacac aaatataatt ttcataatta
    13021 ttttcttttt tagtatccat aatcaaaccc cttctattca tagaatttgc taaaacaaaa
    13081 ataaataatt taaacctaca taatctacaa ttacataata tcccattctt taaatgaatt
    13141 gttttgtata ttatgtatac aaatttataa aaactatacc tataatatta ttctacttct
    13201 aacagcaata tcctccttat atatataatt tattttatta tatgttgtta tattcttagt
    13261 tttttgaatg atatatgtct aaaaaaagac tgtttactaa acagtctttt tttatatata
    13321 tgctcaatat ttaattttca tgatgatgat tacatccaca tccacaattt tcatcatggt
    13381 gatgatgttc ttctaaatct tttacctgtt gttccatttc atgaaactct tttaccatta
    13441 tatcataagt tggaagagct gcctcagtat cgtattcttc ccctctccaa tcagcgtaca
    13501 tcatagcatc cccattagtt aaaaccaatc tacctgacat agttatatta tccacttctt
    13561 ctaaaaattc tatttgtttt tgtaaaactt ctccttctaa ctccacatct tcatctaaac
    13621 attgcactat cattacaggt gcataataat cttcttcatc tttaacattt acaacaatat
    13681 ctaaaattcc ttctgcacct tcttcaaatt ctgccaactc aacatttgta tcaatcatat
    13741 cttgaggtac taataaatct tctattaaat attttattac tctatctttt actaattctt
    13801 ttgacattta tttttctcct tccgattcta taacattatc ataatcattt ctatattata
    13861 gtatactaca ttaattgttt tttcaatctc ttacaaaaat tattttttta ttatcttcct
    13921 tatattccct tttttattac aaagttgtaa tcgcattgat ttaaaaaata gtttatattt
    13981 agtataatag aaatgtagca catattttta ttcaatatat taataaaaaa atagattgaa
    14041 aatgtaatgt atgttaaatc taggaggaat attatgaagt ttaaaaaaca aattagttct
    14101 attgttgtgt ctactatgct tgtacttggg agtatgaacc tttcttatgc agacggtaca
    14161 aatgtagtta cacttggagc taacttatca gagtctcaaa agcagcaaat gcttaattac
    14221 tttggagtaa aaaaagacca agtcttagtt ttagatgtaa caaatgcaga agaaagacag
    14281 tatttacaag gtgttgcaac agagggtcaa cttggaaaag ttactatctc ttgctcatac
    14341 gttgaaccta ctaaaaaagg aaatggaatt aatgttaaaa cagcaaactt aacttgggta
    14401 actagttcaa tgattgcttc aacccttaca acagctggtc ttgaagatgc aaatgttatt
    14461 gctgctgctc catatcctgt aagtggtact ggagcattga caggaatcat gaaagcattt
    14521 gaggatgctt caggaaagaa acttgatgaa actaagaaag aaattgcttc tgaagaattg
    14581 gtagttacag gtgacttagg agatgatata gggcaagaaa aagcaacagg tgtaatgaat
    14641 gatataaaaa cagaaattgt aaagaatggt acaaaagata ccaatcaaat agcaaataca
    14701 ataaataatg ttgtaaataa ttacaatatt actattacac cagaacaaaa agaacaaatt
    14761 aaaggtgtaa tgaaaaaaat agctgaccaa aattacgatt acaatagcat gaaaaataca
    14821 ctagataatg tatcagataa tgtaaatgaa caattaaaga aattaggtga aagtgttaaa
    14881 ggttctggta tactagatac tattggtgga tggtttagtg gaataggaga ctggttctct
    14941 ggtttattct caggcggaga taaaaaagat ttgggaatct taaataacac taatgatgag
    15001 tctcttggct ctgatgctgt aataaattct acagaaggtg ttcaaataaa tccacaaaat
    15061 aatactaatt ctaacaatga tagtgataca aatagctcaa aaaatgatac ttcaagtaat
    15121 aatgaaaata acgatagttc tcaaacaaat gaaaacaaca caacaaataa ttcagaaaat
    15181 caatcaaata ataatgactc agttggtttc tttgaaaaaa taaaaaattg gctttcaggc
    15241 ttattttaga aattaaacaa ataaaaagaa ttgcctaaaa tggaaatttt ctataaaagg
    15301 caattctctt tatttacaat aaatttttat tactcactca tttttttaga tttttctata
    15361 catgcacata ttgcttttat cacagaagct ctaaaacctt cttcttcaag tactttaact
    15421 gcttctatag tagttccacc aggtgaacat accatatctt ttaattcacc aggatgtttt
    15481 cctgtctcaa gtaccatttt tgcagaaccc ataacagcct gtgatgcaaa cttatatgct
    15541 tgtggtcttg gcattcctgc aattactgct gcatctgcta tagcttctat aaataaaaat
    15601 acatatgctg gtgcagaacc acttgcacca attacagctt ctataagata ctctggtact
    15661 acctcagtat ttccaaaaga attaaatact tctgtaactg cttctaagtc ttctttgttt
    15721 atatttttat ttatagaaag agaagacatg gcttcattta caagagctgg agtatttggc
    15781 atagttctaa ctattttttt atcttctcct ataatagact ctatatcttt tatagatttt
    15841 cctgctgcta ttgtaactat aattttattg tcatctataa agtcttttat ctcttccaaa
    15901 atatcatcat atatatctgg tttaacagct acaattacta tatcagaggt tctagttact
    15961 tccttcgagt cttttgtagt atttattcca aaatttgcat ctattctatc gagagttcct
    16021 tgactatatg ctgatgctat aaccatatta ggttcaacta attttgaatt tactattccc
    16081 cctatcatag cgcttgccat atttcctgca cctatgaatc caattttttt cactttagta
    16141 cctccttatt gactaatagt aatcgttaaa atttaagctt ctttaactgc atcagacttt
    16201 tcaaatggat ttggtatact tactaccaaa attttcacta ccttattagt atagttgacc
    16261 caattatgat tgttcttttc actattatag tgtattgtgt ctcctggata catttctatt
    16321 tgttcatctc ccaaaaacac tgtaagagta ccctctaata catacacaaa ctcctcacct
    16381 tcatgtacgt aacaacaaat ttcctcacta ctcttacttg gaagaatttc aattaatctt
    16441 ggtagcattg ttttttcctt taaactagat gataaatgat aatgtataaa tgttgagttt
    16501 tctacatcaa atacttcttt ttcataactt cttaatacag ctcttttatg ctcttttggc
    16561 ttcataaaaa agtaagttaa ttctacatct agtacacttg ctatttttcc taaggaatct
    16621 gttgccacac ttgttaatcc tctttcaagt tgtgataaaa aaccaattga caagtttgtt
    16681 ttttcactca tatccttcag cgtcatttgc ttttgagttc tgagttgctt tatttttgct
    16741 cctatgtcct tattcaaaac catcacccct aaataatttt aattttttca taatataaat
    16801 acaaaaatat aaatataggt actgaaccta tctttatttc atttaaaaaa atttttataa
    16861 gtattactat aatttatcaa tctttttttg tctacatttt tacgattatt tacgatttat
    16921 ttttcacata tgtatattta atcttgccaa ctaaagatac attttttata agcatttact
    16981 ctttatataa taaaacttaa gttgttcatg tagtcttctt ctggtttaat tattaaaatt
    17041 tcaaattata ttattatttc tacacattat tacatccttt agtacaatta taacataatt
    17101 ttacaattta atgatatcta ttcaatgtct ttttttactc tacaacatat tttattttag
    17161 agtttaatac tatatatatt ttttatatac agccttgtca ttttttagat tgtcaagagt
    17221 tattgaagta ttttatgtag atttagattt tatttcctaa tttctcattt aacaagaatt
    17281 gcttatgata aaaaataata tcatctcaat aattgtaaat atatctatat tctaaatata
    17341 atttcttttt ttgtcaaaat ttcttcattc tttaataatt
//
LOCUS       DAEMHW010000072        16954 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEMHW010000072 DAEMHW010000000
VERSION     DAEMHW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.072"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            76..2979
                     /locus_tag="KPK67_002005"
     CDS             76..2979
                     /locus_tag="KPK67_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2458974.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKVSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3012..3458
                     /locus_tag="KPK67_002006"
     CDS             3012..3458
                     /locus_tag="KPK67_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2458975.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            3581..3883
                     /locus_tag="KPK67_002007"
     CDS             3581..3883
                     /locus_tag="KPK67_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2458976.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4064..5470
                     /locus_tag="KPK67_002008"
     CDS             4064..5470
                     /locus_tag="KPK67_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG2458977.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            5814..6872
                     /locus_tag="KPK67_002009"
     CDS             5814..6872
                     /locus_tag="KPK67_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG2458978.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLNYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNAIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            6971..7657
                     /gene="araD"
                     /locus_tag="KPK67_002010"
     CDS             6971..7657
                     /gene="araD"
                     /locus_tag="KPK67_002010"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBG2458979.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            7675..8322
                     /locus_tag="KPK67_002011"
     CDS             7675..8322
                     /locus_tag="KPK67_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG2458980.1"
                     /translation="MLDSLKGKIVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8495..9862)
                     /locus_tag="KPK67_002012"
     CDS             complement(8495..9862)
                     /locus_tag="KPK67_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBG2458981.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10052..10140)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10568..12694
                     /locus_tag="KPK67_002013"
     CDS             10568..12694
                     /locus_tag="KPK67_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBG2458982.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSEIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEDEYE
                     SIPNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFN
                     SFLMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDM
                     KDYSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKILICEDRECNTRKT
                     ISQVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQ
                     YLKNQNKNQETFNNPFAEALAKLKNK"
     gene            12798..14051
                     /locus_tag="KPK67_002014"
     CDS             12798..14051
                     /locus_tag="KPK67_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBG2458983.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNNSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(14205..15266)
                     /gene="ytvI"
                     /locus_tag="KPK67_002015"
     CDS             complement(14205..15266)
                     /gene="ytvI"
                     /locus_tag="KPK67_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG2458984.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            15540..16280
                     /locus_tag="KPK67_002016"
     CDS             15540..16280
                     /locus_tag="KPK67_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBG2458985.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKEKLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            16292..16501
                     /locus_tag="KPK67_002017"
                     /pseudo
     CDS             16292..16501
                     /locus_tag="KPK67_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         6794 a         1891 c         2938 g         5331 t
ORIGIN      
        1 taagtagaaa aaaatatcaa agtaagtatg gcatgatagt tgcttatata tttatcaaga
       61 aaggagttat taagcatgga gttgagagat aaacttgtaa acatcataaa tacagaaaat
      121 aaaaaagatc cattaacaga tgttcaaata gcaaagtttt taagtacaac tagagagaac
      181 ataactaatt tgagaaaaga acttaatata agtaattcaa ggcaaagaag atatccttat
      241 ttaaaatcag caatttcggc aatattacaa aagaataaga atatttctat atcagaaatt
      301 acaagggaat tgatgacaga aggctttaat atatcgagaa gagttgtgga ggaactttta
      361 cctaaagaaa gtttagaaat ggatgtagta gaggaatctg aagaagaatc gaatgaccca
      421 tttgaaactc tgataggtaa taaagggagt cttagaaatg ctgtagagca ggctaaatct
      481 gctatattgt atccaccaaa agggttacca acgttgatta ttggggaaag tggagttggt
      541 aagagtttat tttctagaca tatgtatgag tttgcaaagc aaaaaaagat agtaaaagaa
      601 agctccaatt ttgttgtatt taactgtgct gattatagtg acaatccaca attattatta
      661 tctttgttat ttggatataa aaaaggtgca tttacaggtg ctgaatatga tacaccaggt
      721 cttgtagaag aagctgatga aggagtttta tttttggatg aaatacatag attgcctcca
      781 aaaggtcaag aaatactatt ttctatctta gataggggaa agtttaggag attaggtgaa
      841 actaatgctg aaagaaaagt aagtattatg ttgattgggg caactactga aaatgtagag
      901 actaatttat tattaacatt cagaagaagg ataccaatgc taataacttt gccaccatta
      961 catgatagat tactaaaaga gaaaatagac cttatataca atatctttca acaagagtgt
     1021 aatagaataa atgcaaaaat atttgtagat aaaaatgtga ttgaaatact tgcactaaaa
     1081 aagtttagtg gaaatatagg acaattacaa aatatgattc aggttctttg tgcaagagct
     1141 tttatgaagt ttataaattc caaagatgaa gatagtgaat cgatagtagt agtagatata
     1201 aatgaagttt taaagcttaa agattcgttt aaagatgctg cctttcaaga gattgaatat
     1261 acagagataa gaaaatattt gaaaaatgct atatttatac catttaattt agaagaatca
     1321 tcagtaaaag taagtctttt aagtgatgaa tatatattac cagaggacat atacaaaaca
     1381 attgaaaaga aatattatga cctaaaatcc ctagacattg aagatataga tatagagaat
     1441 attttatgga cttttatttt aaatagattt gctgacttaa agtttaatct tgatagtaat
     1501 gaagaaattt tttcaatgag tgatttaaat agttttgtaa gtgatagttt agttaaactg
     1561 gtaaaagaac ttatgaatga tatagttgaa aaaaatatga atttagaagt aaataaaaat
     1621 atgttcaaat atctagcaat acacttagaa gaagctataa aaagaattaa gctaaatcaa
     1681 aagataataa atgtaaattt agaaaaaata aagattgact tttcaaaaga atatgaaatt
     1741 agtaaaagtt ttgcaataaa aattgaggaa tcaattggta ttaagatttc agatgatgaa
     1801 attggattta taacgctcta cataaagtct gctctaaaaa atgaagtgaa aaaagataaa
     1861 gtagggttaa taataatttc tcatggacgt atagctacag agactgtaaa tgtagttaaa
     1921 gaacttttag gtgtaaagtt tcctgtggct atagatatgc ctttagatga aaaaccaata
     1981 aatatataca ataaggcagt agaattgtca aaaataatag accaagggaa aggcatattg
     2041 tttttggtgg atattggttc acttacaaac ataggtcaaa tagtaaataa gagaaccaat
     2101 ataagtacta aaactattga tagagtagac ctcctaatgg cactagaagc aactagaaaa
     2161 gtttctatgg gagaggaaga acttgatgaa atattttttt ctttgctaaa agataggatg
     2221 ggatataact atgaattaga aaaacacata gataaatcaa atgcaattat aacattatgt
     2281 cttactggag aaggaactgc taagtatata agtaaaacgt tggaagaaaa gtatgataac
     2341 actaaatgtt atcaaatgag tgcattggat gaaaatttat tttgtaaaat tgaaaatcta
     2401 aaagagacaa acaatatatt agctattgtt gggacaataa accctaagat accaggtata
     2461 aattttatac cctataataa agaacttttc aaaaatttgg atatctatct atcaaaatca
     2521 aaacaacaag atgacagacc aattaaatcg tatgaaagaa tgcttgatga ggatttagtg
     2581 atttttgaac cagatatata ttttaaaaag gatttattag aatatgtatg ttcaattctt
     2641 ataaataaag aatatgtaga aaaagattac ttagattctg ttttacatag agaagaaatg
     2701 cttccaacat attctaaagg agctataggt gtgcctcatg gagattcttc tactgtaaat
     2761 agtacaaggt ttgtttttgt taaactaaag aatcctattg attggggtgt aggtaatgta
     2821 aatttcatat ttatgccagt ttttcaggct aatgataaag agattgttaa aaatgttctt
     2881 gagatactaa aagatgcaga atttatgaac aatgccaata agtgttttga taaagatgct
     2941 tttagaagga ttatatttga taaatttaaa catctgtagc catagcaaaa aaattgagag
     3001 gagttagagt aatgaatgta gatttaatat cattgggttt agaatctaat gattatgaaa
     3061 gtgttattga agaattagga agtataatgt gtaaaaaaga atatgtaaaa gaaacataca
     3121 taaatgctgt cttggaaaga gaaaggacat taccaacagg tctagatatt ggagaaatgt
     3181 gtgttgctat tccacataca gattcaaagc atgtaaatga gtctaatgta gctgtaggga
     3241 tattgaaaaa tcctgtaaag tttaattcaa tgatagaccc aaaagataga cttgatgtag
     3301 aacttgtatt tttattagca gtaaagaacc cagattcaca agtaaaactg ttaaaggatt
     3361 taatgtcagt ttttcaaaat ataaagttat taaaaaatat aaaaaacgca tcaacaaaag
     3421 aagaagtagc taaattattg gactttatag aaatttaatg taggagaatt ttataaataa
     3481 aaaattaaaa tttaaacaat caaagtaagt attatttttt taataggttt aatcttatat
     3541 aaaataaaaa tttagagtga aaaattagga ggaaataatt atgaaacaat ttaatgtatt
     3601 atcagtttgt ggttcaggga cagttacatc atctatggta gcaggtaaat taaaagaagt
     3661 actaggtaat aaggggattt ctattactac aacagaagct agaccaacag aagcactaaa
     3721 tttagcacaa tcaggaagat ttgactttat aacatttacg agtccacttc aaacaggcga
     3781 ttatggaata ccaactataa atgcatttgc ttgcctaact ggtatagggg aagaggcatt
     3841 ctttgaagaa gttttgactg ttattgaagg gatagataag taagttttgg agacgtgaat
     3901 ttatagtatt taaatcaatg atttagatac tataataagc taatattaac taaatatttg
     3961 tggaaaaaac attttatatg ttgtcataaa ttaaaaaatt ataaaaatag tcacttaagt
     4021 attaaattaa gcttataaat atttataagt aggagggaaa aagatgttag aatttatgaa
     4081 atctataatc gatacatttg gttctgcaat tatagttcca attataatat tcataatagc
     4141 aaagatattt aaagtaacta ctaagaaatc gtttctttct gctgtgtatg caggagttgc
     4201 tttacaaggt tttacactga ttttaaattc atttacacca ataataacac cagttatcaa
     4261 taggatggtt gagagtacag gagtaaacct accagttttt gatgttggtt ggcaagcaac
     4321 atcattggtt gcattttcaa caagtgcagg tatgatatat ttaggtcttg gtatattatt
     4381 acaaacaatt ttatttttga taaaatggac agacgtgttt caaccatcag atttatggaa
     4441 taactattct tatatggtat ggggagcaat ggttatagga gtaacaggaa attttccttt
     4501 aggtatagca tgtatggttt tattaaacct atatagttta ttaatatcag agttagttgc
     4561 aaagagatgg tctagttact atagatatcc taattgtaca ataatagcta tgcataatgt
     4621 tgaagcttct gtgtttgcag tatttgcaga ccctatatac aataaattgg gattaaataa
     4681 aataaaatta aatccaaaag aattagagaa gaaattaggg tttttaggtg aaccaattac
     4741 tttaggtctt tttttaggta tgtttatagg tatattaggt aatatgacga gaataaatac
     4801 tatggaagct tggggagaaa taatgaaagt aggtatatct acaagtgcag tgatggcaat
     4861 tttccctaaa gtagcaagta tgtttgctca agcatttgca cctataacag aagcagctag
     4921 aaagattatg caaaaagctg gaaaccgtga gtggtacata gcagtaaatg atgctgttgg
     4981 atatggggaa cctgcaactt taatatcagg tcttatatta attccaataa tgcttgtaat
     5041 agctatggta cttcctggta ataaggtatt accagttgtg gatttattgg caattccata
     5101 tatggttcaa ggtttagttg ccatacataa tggtaatata ccaaaagtat tagtttcagg
     5161 tataatttgg tttggattag gattatatgt ttgtacatct acagcaccat tatttacaga
     5221 tatggctaca aatataggtg tagctatacc tgcaggagca atgttaataa caagctttaa
     5281 tatactagga aaacctttga tgggattagt tttctttgca tttctaagtg caaatccaat
     5341 ttatataggt ctttcagttg taatttactt tgttctatgg gcattgttta gaaagaacaa
     5401 aacaagtatt cttgattatt tagaaaaaca agcactcaaa aatgtagaag aagagcctgt
     5461 agcagtttaa gtttataaaa atgaaataag aataattaaa ggagtttata attgatttta
     5521 tataagtatc agataatacc tgagaaatat ttaaatgaaa tatagacttc tttttatttg
     5581 ataatatttg taattataat gcattgtatt tttatagttg agttttctgt ataacctgct
     5641 atttaaaaca tactctttta tatttataca gtaatgtggt ttaattttat aaaatgtaat
     5701 ctctactggt aaaagacttt gtatataagg catttgcctg aataaaccat ttatcttttg
     5761 tatggcatag atattgctat atagaagtat atagcatgaa aggagatatt tatatgggag
     5821 ataaaatgag agcatcagta ttgtataatg taggtgatgt cagatatgaa atggtagata
     5881 taccagaaat aacagatacg caagtgcttg taaatgtaaa atatgttggg atatgtggtt
     5941 cagatttacc taggtctatg gtaagtggtt tgagtggaaa tacaaaatat ccattgattt
     6001 taggacatga attttctggt gaagttgtta aaattggaga aaaagtaaag catattaatg
     6061 taggagatag agtagctgtt gcaccattag taccttgtgg aaaatgtgat tattgtaatg
     6121 aaggtaattt tggcttgtgt gatgattaca atataattgg aacaagagta aatggagcat
     6181 ttgcagaata tgtaagagtt ccagaagaac atattttaaa actaccagat acattgaatt
     6241 atgaaactgc tgccggaatt gaaccagcaa ctatagctta tcatgggatt tcaaagtcta
     6301 atattagagt aggagatagt gtagttgtgt taggatgtgg tccaataggt cagtttgtta
     6361 tacaatgggc aaaggtgttt ggtgcatcta agattatagc agttgatatt tttgatgaaa
     6421 aattagaact ttctaaactt ctaggagcaa actatatact taactcaaaa gaagtaaatg
     6481 caatcaaaga aataaaaaaa ataacaaatg gtggtgctga tgttgtaata gaaactgctg
     6541 gtagccgatt tacacaagaa caatctctat ttgtagcaaa aaagagagga aatatagtgt
     6601 ttgtaggtat atcacacaca gaattaccac ttagtgcaga cgctactgag tgtatattac
     6661 gtggagaact taccttgaaa ggttcttgga attcatatac aagcccttat cctggaagag
     6721 catggactgc aacattggat tttatgggaa aaggtgacat tatttttaaa cccatgattt
     6781 cacataaaat aggtcttaat gaagtgggtg actttttaag taaaatgagc aagagagaaa
     6841 ttaatttcaa taaaatccta gtagaaatat aaaaatttaa taaaaaattc taaaagcaat
     6901 taaattttat aactatgaca ttaaataaaa taaaaataat tttcttaaat aaaatatagg
     6961 aggaagacaa gtgttagaaa atttaaaaga agaagtatta aaagcaaact tagaattacc
     7021 taagaaaaag ctaattactt atacttgggg aaatgtaagt gggatagata gagaaaaagg
     7081 attagtggtg ataaaaccaa gtggtgtaga gtatagtaat atgactgtag aagatatggt
     7141 tgtagttgat ttagagggaa atatagttga tggcaaatta agaccttctt cagatactcc
     7201 gactcatcta gttttatata aagagtttga agaattgggg ggaatagtac atacacattc
     7261 aagttgggca acaatttggg ctcaaatagg aaaatctata ccatcttgtg gtactactca
     7321 tgcagattat ttttatggaa gtataccatg taccagaaaa atgactagag aagaaatttg
     7381 caatgaatac gaaaaggaaa ctggaaatgt aatcattgaa gaatttatgg gaagaaatcc
     7441 aattcattgt ccaggaataa ttgtaaatga ccatggacct tttacatggg gaaaagatgc
     7501 aaatgaggct gtacacaatg cagtagtttt agaagaagtc gcaaagatgg cttattatac
     7561 agagttaatg agtcctgata atattatgga taaggttctt atgaataaac atttttcaag
     7621 aaaacatgga aaaaatgcat actatggtca aaaatgatta tctggaggtg aattatgcta
     7681 gactctttaa aaggtaaaat tgtggaaata gcaattaaag cagagcatta tggtttatgt
     7741 aaagaaaaga ctggtaattt tagtattaga gataaggata ctggatatat acttataacc
     7801 ccatcaggta taggaagaga agaattaaaa gttgaacata tttgtgtatt ggatttggat
     7861 ggaaatgtaa tagaagttga gaaaggtatt aagccaagta gtgaggtatt gatgcatatt
     7921 gaaatataca aaacaagaga aaatataaat gccatatcac atactcattc tttatatgca
     7981 acagcatttt cagtagcaaa gaaaccaatt ataccaattg tatatgaatc agttaattat
     8041 ggtggctatg tagatattgc tccatacgaa agaccaggaa ctttaaaatt agctaagagt
     8101 gtttcaaaat tactgataga tacagatgca tgcttattag aaagacatgg gttgacaact
     8161 gtaggtaatg atttagacga agcattatta aaatcaagat atgttgaaga ggtagcagaa
     8221 atatactata ggtctttagt attaaatcaa tttaaagaac ttgaagtagt agatgttgat
     8281 gagtttaaat cttggaaata cccagaagaa ataaagtttt aatctaaata attttcattg
     8341 aagtggttaa ttaaaatata tgtattattt atatcaatta ctatgtttta ttatattaat
     8401 taccgtattt tgtaaatata tttattacat tttttcaaaa aaatatagaa tccgaaagcc
     8461 atataaaatt gacttgggat tctatacttt ttatttatgc atttttcttt atctgatttt
     8521 ctggagaatt tatatgttca aaatattttt tagtttcaga tactattata ggtgataatg
     8581 caagtaaagc tataaggtta ggtattgcca taagcccatt tacaatatca gcaattatcc
     8641 aaacaacttc taactttaag aaagcaccta ataacaccat agccacaaat acagttctat
     8701 acccattcat gccttttaca ccaaacagaa attcaaaaca tctttctcca taataactcc
     8761 atcccaaaat tgtagtaaat gcaaataatg ttaaactaac tgtaagtaat attggtccaa
     8821 atgtaggaat tgctccgtta aaagctgctt gtgtcataac agctccattt aggtctgaac
     8881 accacactcc tgaaataaca agagagaatc cagtaagagt acagattata atagtatcta
     8941 taaaagtacc tgtcatagat ataagacctt gttccgctgg ccattcagtt ttagcagcag
     9001 cagcagctat tggagcacta cctagaccag actcatttga aaatacacca cgagcgatac
     9061 catttctaat tgccagtgac atagttgctc ctaaaaaacc accagtagca gcagtaggtg
     9121 taaatgcact tttaattatt aacataaaag tttcagggat tttattccaa aaagatatta
     9181 aaatgattcc acagattaca acatatataa ctgccataaa aggtactatt ttagtagcta
     9241 cattagatat acttttaagt cctccaaata caactaaagc tacaaatact gctaatacaa
     9301 cccctgtaat tctagggtct atcccgaaac tattatttat tgcatctgtt attgaattta
     9361 cttgagtaaa agtaccgatt cctaaaagtg caactagtat accacttata gcgaaaaata
     9421 ttgcaagagg tctccatttt tctcccatac catttactat gtaatacatt ggcccaccag
     9481 acacttgacc atttttatct ttagttctgt attttattgc aagaacacct tctgaatact
     9541 tagtagccat tccaaagaat gctgctatcc acatccaaaa aagtgcacca ggtccaccag
     9601 ctttgatagc tgtagcaaca ccaacaatat taccagtacc aactgtagca gctagagcag
     9661 tacataaggc tgcaaaactt gaaacatcac cactaccttt gttttcagca gagaatatta
     9721 acttaagagc tcttggcagt ttagttattt gaacaactcc aagcttaaca gtaagtagta
     9781 taccagtacc aactaatagt accagtaggg gtggtcccca aataaaagca tcaactttgt
     9841 ttaaaatatc aattagattc ataaaaacac tccctcattt tttttgcttt acataatcaa
     9901 tagaattttt aatgaagtaa ttgacaggaa ataagtataa tttattaata atttctagtg
     9961 taataaaaaa agctaaatct aaaaaagatt cagctctgga tgtacactaa ttagcaacca
    10021 aaataaaagt attagtaaat actgtttttg gtagataatc cctgtccttt tgcctgagag
    10081 attgagcata aaagccttgc cccttcggcg cccataatgg gtctctccag agttccgtaa
    10141 attttacagt cctactttta aaagtacctg agagtgttac tccttcggtg tgctatatag
    10201 caactcttct ataaaatctt aaccggatat tgatttgtca tagcttattg tattaataat
    10261 attaatataa gtttttatgt tttgtcaact aaaaaattaa aaaacaaaga aaaaatataa
    10321 aaatgtaagc ctatttacga attataaact tagaaagatg agtaatatac gtgaaaatga
    10381 attttgtaat acacatacaa atgaagtgta aaataagttt taaataaaaa aataataaaa
    10441 tcttaaataa ttaattagat atgattaaat tatggtaata ttatagttac gatattaaga
    10501 aaagttctat ttagttagat tttaagcgta atatgagatt atgtatagag aaaggatgta
    10561 tttttagatg ggaaaaatat tagtgctagc agaaaaacct tctgttggaa gagatatagc
    10621 aaaagtttta gggtcaaaaa atgaaaaaaa tggttatata gaggggccaa agtatgtgat
    10681 aacttgggca ctaggtcatt tggttacact tgctgacccc gaaagttatg gagaaagata
    10741 taaatcatgg agcttagaag acctgccaat acttcccaag caccttaaga ctgttgtaat
    10801 aaaaaagagt ggaaagcagt ttaatacagt taaatcgcaa atgaatagaa atgatataga
    10861 tgaaatagtt atagcaacag atgcaggtcg tgaaggagaa cttgtagcaa ggtggataat
    10921 agaaaaatca caggtaaaaa aacctataaa acgtctttgg atttcttcaa gtacagacaa
    10981 agcaataaaa gacggatttg caaaattaag aagtggaaaa gaatatgaaa atttgtatta
    11041 ctcagcaata gcacgagcag aagctgattg gattattgga ataaatgcaa ccagagcttt
    11101 gacaacaaag tacaatgcac agctttcttg tggaagagta caaactccta ctcttgcaat
    11161 gttgcttaaa agagaagaag aaataagaaa tttcaaacct aaagagtatt atggattaga
    11221 attaattgct acaaagggaa attcagagat aaaatttatt tggaatgata aaaacaataa
    11281 ttcttcaact tttagtaaag aaaagataga atccacatta aaaaaagtaa aaggtgtaga
    11341 tataaaaata tctgaagtaa acaagtcata taagaaaaaa tattcacctg cactctatga
    11401 tttaacagaa ttacaaagag atgcaaataa aatgtttgga ttttcagcaa aagaaacttt
    11461 atctataatg caaaaactgt atgaacatca taaagtactt acttatccta gaactgattc
    11521 tagatattta acatcagata ttgttggaac attaaaagat agaataaaag ctgtaaatat
    11581 tagtgattat tcaaagattt gtaatagatt attaaaaagc aatattatag ctaataaatc
    11641 gtttgttgac aattcaaagg taacagacca ccatgcaata ataccaacag aacagaaggt
    11701 attcatgagt gattttagtg ataaagagcg aaaaatatat aatcttgtag taaaaagatt
    11761 tttagcagta ctatctcctc cttttgaata tgagcagaca actataagtg caactgtaga
    11821 aggagaaata tttaaggcta agggaaacaa agtaataaat ttaggttgga aagaaagtta
    11881 taaagatatt gaagatgaag atgaagatga gtatgaaagt ataccaaatg ttttagaaaa
    11941 agatgtactt aaggtcaagg gattaaagat aacttcagga aaaacaaatc caccaccata
    12001 cttaaatgag ggaacattat tgactgctat ggaaaagaat ggtctaggta cagttgctac
    12061 aagagcagat ataatagaaa aattattcaa ttcattttta atggaaaaga aaaataaaga
    12121 tatatatata acatcaaaag gaagacaatt attagattta gttccagtag acttaaagac
    12181 accagaactt acagcttcat gggaaaagaa attattggat atttcaaagg gaaaacttag
    12241 taaaaataca ttcataaatg acatgaaaga ttattcaaaa gatattgtac tagaagtaaa
    12301 aaatagtgat aataaattta aacatgataa cttaactaaa aatagatgtc cagaatgtgg
    12361 taaatttatg ttagaggtaa atggtaaaaa aggtaagatt ttgatttgtg aagatagaga
    12421 gtgtaatact agaaaaacta tatcacaagt aaccaattca agatgtccta actgccataa
    12481 aaaattagaa cttcgtggag aaggtgaggg gagaatcttc acttgtagtt gcggatacag
    12541 agaaaagttg tcttctttca acaagagaaa gcaagaagaa aaacagaagg catcaaaaaa
    12601 agatgttaat caatatttaa aaaatcaaaa taaaaatcaa gagacattta ataatccatt
    12661 tgcagaagca ttagctaagc taaaaaacaa ataattttaa agttattatt atacacaatt
    12721 ataactttaa ctgtatatta gttatatgta ctgatataat atagtgtata gtttaggcat
    12781 gaaagtaggg ctgttaaatg gatttaagtg gcatatttaa atactattgt aaagagtgtg
    12841 aaaatacatg gaataattcg agtgttgaat tatttgagaa tatagaaacg tatagtaaag
    12901 attcacaaaa aaagagggaa aaagaattag ataaattgct aaatacaata tcagttcatt
    12961 tagagaggta tccaagtgat gctgtattga gaaaaatgtg ggtaaaaaag ggcgaggttt
    13021 ttttacaaaa gacattggaa aaagaaaata tttttaagtt agaaaaaatg gatgtagagg
    13081 atagaaaaaa atttttagat ataacaaaac agtttattag agatgctaga aaatttgatg
    13141 atgatttacc tataggtgat attatgcaag ctatgaggaa tgtatggatt tcaaatgcat
    13201 tacaattatt atttggtaaa gaagtatatt attcaaaagc taactttgca tatagtatgt
    13261 tatatccata tactgataat tatttagaca atacaaatat agataaaaat gataagattt
    13321 tatttaataa ctggttagaa aaaaggctcc tgggagaaca cattaaatct aaggattatc
    13381 atgaaagtaa agtatctcaa atgatagatt atattgaaag tgtataccct agagaaaagt
    13441 ttacagaagt ttatgaatcg ttattattaa tatttaaaag tcaagtaaat agtttaaaac
    13501 aacatggtaa ggaaaatcat ttgtgtaaag aagatttatt atccatttct attgaaaaag
    13561 gaggttcatc cgttttagta gatggatatt taataagtgg attgatgaca aaggaagaaa
    13621 tagagttttg tataggatat ggatttttat tacaaatatc tgatgattta caagatataa
    13681 aagaagattt aaaatacaac cataaaacta ttattacaga gatgtcaaaa gagggtactt
    13741 tagataaagt tgtaaataaa ctaataaatt ttactattga gttaatagat agttttaaaa
    13801 ttaataataa aaataactct gtaataacta tgataaagaa tgattgctta atgttaattt
    13861 tattttctgt agtttataat gctgaatttt tttctgtagg atatataaaa gaagtagaga
    13921 aatttattcc atatacaata gattattcat tagagattga agaaaaaata aaagaaaaat
    13981 ttaaaaatat agatgtttta aataatgaaa atgaatataa agaaatgatt gatattattt
    14041 gtgcagagta gaaaaaaagg gagtgtctta gaatagactt tttagtttat gaggcactct
    14101 ttttgtataa aatcaaatgt attttcatca ttttaacaat taaaaagagc ttatccctat
    14161 ttgagaaagc cctttttaat ttaaataaaa atgtcaaggt taaactattt aaatgcagta
    14221 ataatcccat tatcatgtaa gttttttatg attgtagctg cgatagggaa tccaaataat
    14281 ccagctattc caaatagttg agctccaaca aacatacaca ttaatgttat taaaggatat
    14341 agaccaattt ggtgtcctac tacctttggt tctaatattt gtctaacaac ggttataatt
    14401 atgtatagta ttaaaagtcc tattgctaaa ttaatatttc cattaatgag tgagataatc
    14461 atccaaggtg tcaaaatact tcctgtaccc aagactggca aaatatcaac aagtgctatg
    14521 atgacagata tagtaaatgc attttcaacc tttaggatag taagtccaat aagtagttct
    14581 atgaatgtca ctgatagtag tattgcatag gcttttagta gttttaaaag agcattaata
    14641 ctgctatttt ttatatttat tatcataaat tgactttttt ctggaaattg ttttaagata
    14701 aagtttacta ttttttgata gtcaaaagta aagaaaaaag aagcaattaa agtaataata
    14761 agttttaaca taaatgcagg tgttttactt gcaatactag caatagcatt gagtgcattt
    14821 gttgaaatag aagctactaa agacatcata gattgactta tattatccca accaagtatt
    14881 aatgctaggt taacattagg tgtactagca tctatctttg aaaataaagt atttagcgtt
    14941 ggttgtatac ttgatttata tatttcagga agtctaaaaa atacatcact tacacttgca
    15001 aatatcttca caccaaagct aactaataaa aatatacaca acccataaaa aattaaaagt
    15061 attactatag aaataagtgt acgatttaaa tttgtattat ttgtaataag tcttataata
    15121 ggtcttaaga tggatgcaat gataaaactg attacaaaag gcattaataa tggcaataag
    15181 aattttaata caatatatac aatacttgtt attaaaacta catatgtaaa tttaattata
    15241 aaattctttt gcttttgaat atccataata taaaacctcc aatgtatttg atattattct
    15301 aaatatggtc ttttttacag aaaatatact actgtatatt gattataaac tataaagctt
    15361 atgtaaagta aataaattat gtaaaattta ggttaatttt gtataaagag atacttttat
    15421 attttacaat taataaccaa atactaaaaa atattttaaa taaagaactt aaaattgtga
    15481 taaaattaat ttatagatat taaaaaaagt ataatatatg gtttttaaga ggtgctaaga
    15541 tgaaaaattt tagagattta ggtgggaaca aaactgagga tggcagaact gtaaagaagg
    15601 gattatttta taggtctgca aaattatcaa atctaagtga aaatgatata aagattttaa
    15661 aagagttaaa tattaaatat atatttgatt atagaagtga tgaagaagct cgtaaacatc
    15721 catcaacaat aatatcaaat ataaaaaata taagaatacc agcaatgaga gagctagaag
    15781 aatcaggagg aagttttggt tctattgaag atatgattga tggattgttt gaaaaagatg
    15841 gtgcatttaa tatgttaaat aatagttact ataatttgcc tataaataat ccgtcatata
    15901 aaaaattagt agaacttata agagactatt ctaatttacc aatattaaat cattgtacag
    15961 ctggtaagga tagaactggt gtggggagtg ctataattct tatgatttta ggggtatcga
    16021 gagagaatat aatgaaagat tatctaaaaa gtaatgattt tgcagataaa gagatagaaa
    16081 gatttataga atataagcct aaatttaagg acattccaaa agaaaagtta aaatatatat
    16141 ttggtgtaaa tgaagagtat atgaaaactg catttagaag aattgatgaa gaatacatta
    16201 gtgtagaagc atatttatat ggagaattta atttaaataa agaagaaata agaaaattaa
    16261 gaaatcaata tctagagtaa aaggtgagtt tatgagttta aatataacag caagacagat
    16321 aaatataata aaaatattgt taaattcaaa agatacaatt agtggtattg ccttaagtca
    16381 agagatagga tgttcttcaa aaactataca aaatgaaata aaggatatta ataaacaact
    16441 tagaaatggt aaaattttgt caataagagg agttggttat aaactagaag gaagttttga
    16501 ttaaaaaata gctacgcaca cttgtgactt tagtcatgag ttagtagcta aattagtcag
    16561 catataggga aacttatatg tagagatagg atagaaccta tccaatacta cttgaattgc
    16621 taaaaacccc taaagctaac caaactaaaa cataaggatg aaataaacct aagtgtgaaa
    16681 gttacgaaag tagaaaaaat tggttagatg gtataaggtt aaatcctaag tactttaaaa
    16741 tgggcaatca gcaggtaagc tccgaatagg agaaacttca acgactattc ctcttgaggg
    16801 aagtacacta caagctattg gtagtggaag tgggtagacc ttaacggata atgccgaagg
    16861 ataagatata gtctgtgctt acatgaaagt gtaagaagtt cataagagaa ctgcataggt
    16921 agtagcgaac ttatgtgaac gacaacctca aaaa
//
LOCUS       DAEMHW010000073        16919 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEMHW010000073 DAEMHW010000000
VERSION     DAEMHW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16919
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.073"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(310..1041)
                     /locus_tag="KPK67_002018"
     CDS             complement(310..1041)
                     /locus_tag="KPK67_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2458986.1"
                     /translation="MKFSHKLNNLSHAEKHIFYYIDSSIHTVKDLSLTALADINNVST
                     TTIIRMCSKIGLSGYSELKHILKDLDKESTSISSGYHVSNIIDNLNQNLSNLNLTNIN
                     KLAKNIKSANRIIIVAVGLSKPIGEYFSKLLMQANKNSFYVYESHMIDLLDQTTEKQD
                     LVIFVSNSGETRTLITACEKFRYKNLNTAAIINSPNSTLSMLVDIPINTCSQKLNISG
                     YDTTPRSTILVIIDILFETYLNIIE"
     gene            1194..3164
                     /locus_tag="KPK67_002019"
     CDS             1194..3164
                     /locus_tag="KPK67_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2458987.1"
                     /translation="MNLAKMTNKNLIVLDVSVATKEEAIRLLIDKLYKENIISSKEKF
                     FDTVMEREKHSPTGLERGLAIPHGKCDSVNRSAFAVARLNNKINDWESIDESNEVDLV
                     FLLAIPKSEKEITHLKLLSELSVALMDENFYNDLSISKNADEFLKNLNRSDKEEKDTN
                     KYNKTVLVVTACAAGIAHTYMSAEALEKAGKEMGIKVISEKQGASGIEDRHTKEQIQK
                     ADGVIFACDVAVKNIERYQGKSFVKVRVAEPLKHSKNLLNKVLQNPDGKVEVLSNEEA
                     FTSTYESKQGTLKEMMEAVMTGISYMIPVIVSAGLMMGIAKLTAVAIGQIDNMGTFIE
                     SGNAFYELLGYLDMFGGRIFKFIYPIFSAYVAYSIADRPALVPGLIGGAFAGGLHFTF
                     WGVEGGVPSGFIGALILGLVAGYVTRFLNENIKLNKNLQAMKPMFLLPGITMLVIFLL
                     NFYAVDPAFGRVNAFIANIIESFGTASTIALTVVIAACTAFDLGGPVNKAAGAIAIGL
                     AADGVFPLTARVLSIVIPPIGLGLATVLDKIIVKRRVFDDNLRVVGNSSIILGLIAVS
                     EGAIPFMLKNPLITIPINILGAIIGSITAVMLGAIQWNLLPATWGWPLVENLWAYLVG
                     LIAGTLFIALANIFIRFNIIKKQNKNDINKTS"
     gene            3271..5949
                     /locus_tag="KPK67_002020"
     CDS             3271..5949
                     /locus_tag="KPK67_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBG2458988.1"
                     /translation="MEEDMNNRKKVYVVTHSHWDREWYFTIEDSNILLSENMPYLMDV
                     LEKDMDYTSYTFDAQLSVVEELVKLYPEEKERLKRLVSDKRIFVGPWYTQTDSILVNK
                     ESIIRNLLYGTRLGNKYGHSMKVGYLPDIFGQNAYLPSIFNGFDIEYSVLQRGIYIDD
                     LKGNLNFKWVSPDNKSVRANNIFLGYGPGKFLSSDNQYIQDKLIPMLEKLESLNRDCN
                     NLLLPCGGDQVLIRKQFPQIIKELNEKQDKYKFVLSDYETFMKDTWTNDFENKIHGEL
                     IACEKSRIHNTIKSQRYDIKYLNTLVENKILYVLEPLSVIGKFSGISYKSRWLDIMWK
                     LLFDAHAHDSIGGCNSDETNRDIVKRLKKVNAMCDDIINIVKKQITMAISKYLNIDNI
                     LVVFNTKNKYMNEIIEAVVFTKKKDFTISTIDKEMVELTINKQEFISGGKQILVTAEG
                     EKEVELLGYYRTEVRLANVNVKPMGFTTLVVNQDSKEIADMLVASNDRSVENDLYKLV
                     FDNNNLLLVNKYTNKRIDNIVHFENCGDYGDSYDFSPLDNDKDILATKAELLEVSKSN
                     LVQTMKLKHSIELPFDIDERKLNKKSARLEIITRIEIRKGENFIRVSHDIENTVKDHR
                     VRVVYNTNVKSKVSLSDQGFSAITRQNYNRHLGNWQERKFAEKPVCIYGLENFVALRD
                     NTNTFALVTKGIKEYEILEDKKIALTLYRSVGLLGRDNLAWRPGRASGINNKVVYTKD
                     AQLLNRKLKFEYAIYYDKSDEDVTELFDVTDKFINKYTTYQNQDLNLFEERLERFEIP
                     KYHTINLSSYSLFNIDNDGIFMSVCKESYEEDGIIVRLFNPTSEAKEVSINSEYIKDI
                     KITNLYEKELDILDSNVQIKAKGYITLKLLGGEYNE"
     gene            5942..7636
                     /locus_tag="KPK67_002021"
     CDS             5942..7636
                     /locus_tag="KPK67_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBG2458989.1"
                     /translation="MNKTILLQDIEQKLKNIYKEKYKPKFFDMMKSTIEKFEGKAFDK
                     VDVISEKNVYLITYGDSIYEEGIPTIHTLKKFLNNKVGDTITDVHILPMFEYTSDDGF
                     SIVDYMKIDKKLGNWESINNLSKDYRLMYDFVANHVSKNSKWFQGYLNGEEKYQNYFI
                     SEDKNFDYSNVIRPRTSPIIHEYQGKDGVKTAWTIFSEDQIDINVKHFPVLIEITEIL
                     LNYALNGASSIRLDAIGFLWKESGTTCIHLPQTHMIIQLWRIILDYFKKDTQIITETN
                     VPHLENVSYFGDTTNEAHMVYQFTLPPLTLHTFTTHDSTKLTKWASTIGKVSNNATFF
                     NFLSSHDGIGMRPTEGILTEEENKRLVDKTIINGGRVSYKDNPDGTKSVYELNINYLD
                     ALLNKDEDIDEDVQIQKILSAHALLLSFIGVPAIYYHSLLGSKNYYKGVEKSGINRRI
                     NREKLEYNRICYELEHDSRRNKIFTQLKNMIDIRKGESAFSPYANQNVLDFGKEIFAL
                     ERENKETNEKVVFITNVTPNEKIIDCGFNGINILENINIEGKLTLGAYKFVWIKIK"
     gene            8928..9302
                     /locus_tag="KPK67_002022"
     CDS             8928..9302
                     /locus_tag="KPK67_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2458990.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMVDMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            9304..10161
                     /locus_tag="KPK67_002023"
     CDS             9304..10161
                     /locus_tag="KPK67_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2458991.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKSMPDILIERPTIEDI
                     MLAYIERRE"
     gene            10164..10802
                     /locus_tag="KPK67_002024"
     CDS             10164..10802
                     /locus_tag="KPK67_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG2458992.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMMCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHNNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            11808..12542
                     /locus_tag="KPK67_002025"
     CDS             11808..12542
                     /locus_tag="KPK67_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2458993.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            13376..14632
                     /locus_tag="KPK67_002026"
     CDS             13376..14632
                     /locus_tag="KPK67_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG2458994.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            14695..15888
                     /locus_tag="KPK67_002027"
     CDS             14695..15888
                     /locus_tag="KPK67_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG2458995.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIAGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGENK"
     gene            16089..16469
                     /locus_tag="KPK67_002028"
     CDS             16089..16469
                     /locus_tag="KPK67_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2458996.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
BASE COUNT         6445 a         1778 c         2852 g         5844 t
ORIGIN      
        1 taacattaga attatatcaa gaaggtgaaa atacaactcg atagtgagaa agagatttat
       61 atctagtttg agataagtct ttttaattat taaagttatt ttttatctta tttaaaaaag
      121 agcatcagta ttttataaat tagtattttt tataagtagt ttatcactta atttatcatt
      181 aaacaaatca tttatacagg attaatattt cttttatata ttcaattctt acttttaata
      241 aatctttaat atattccatc ccatgtagtt ttaaagaaga catttttact tataactata
      301 tcttttttat tattcaataa tatttagata agtctcaaat aatatatcta taataactag
      361 tatagtagac cttggagtag tatcgtatcc agaaatatta agcttttgag aacatgtatt
      421 tataggtatg tctactagca tagatagtgt agagttaggg ctatttatta tagctgctgt
      481 gtttaagttt ttgtatctaa atttttcaca tgctgtaatt agtgtgcgtg tttctccact
      541 attagataca aatattacta agtcttgttt ttctgttgtt tggtccaaca agtcaatcat
      601 atgagactca tatacataaa aactattttt attagcttgc attagcagtt ttgagaagta
      661 ttctccaatt ggctttgaga gccctactgc aactattatt attctattgg ccgatttaat
      721 atttttagct agtttattta tatttgttaa attaagattt gatagatttt gatttaagtt
      781 atctattata ttactaacat gataaccact agatatagat gtagattcct tatctaaatc
      841 tttaagtata tgctttaatt cagaataacc tgataatcct atcttagaac acattcttat
      901 tattgtggta gtgcttacat tatttatatc agctaaagct gtaagtgata aatcctttac
      961 tgtatgtatg ctagaatcta tatagtaaaa tatatgtttc tcagcatgac taagattatt
     1021 caatttatgt gaaaatttca ttatgaaact actaatatta atcaccacct ttgtatatag
     1081 taaaaaagta caaatgtact ttttatgtat aagaaaatgt ttttcatcta atatataata
     1141 atattataag ataaataagc atttaagtaa atgtattaat aggagggtga gatatgaatt
     1201 tagctaaaat gaccaacaaa aacttaatag tgttagatgt aagtgtagct acaaaggaag
     1261 aagctataag gttattgata gacaagcttt ataaagaaaa tattattagt tcaaaagaaa
     1321 aattctttga tactgtaatg gaaagagaaa agcattctcc aactggacta gaaagagggt
     1381 tagctatacc acatggtaaa tgtgactctg taaataggtc agcttttgct gtagctagat
     1441 taaacaataa aataaatgat tgggaaagta tagatgaaag taatgaagtg gatttagtat
     1501 ttttattagc tatacctaag tctgaaaaag agattactca tttgaagtta ttatctgaat
     1561 tatctgtagc tttaatggat gaaaatttct ataatgattt aagtatttct aaaaatgctg
     1621 atgagttttt gaagaattta aatcgtagtg ataaggaaga aaaagataca aataaataca
     1681 ataaaactgt attggtagta actgcttgtg cagcagggat agctcataca tatatgtctg
     1741 ctgaagcttt agaaaaagca ggtaaagaaa tggggataaa agtaatatca gaaaagcaag
     1801 gtgctagtgg tatagaagat aggcatacaa aagagcaaat acaaaaagca gatggggtaa
     1861 tatttgcttg tgatgttgct gttaaaaata tagaaagata tcaaggaaag tcatttgtta
     1921 aggttagagt agcagagcct ttaaaacatt ctaaaaattt acttaataaa gtacttcaaa
     1981 atccagatgg aaaagttgaa gttttaagca atgaagaagc ttttacatct acatatgagt
     2041 caaaacaggg tacattaaaa gaaatgatgg aagctgtaat gacaggtata tcatacatga
     2101 ttcctgtaat agtttcagca ggtcttatga tggggatagc taagcttact gctgtggcta
     2161 tcggtcaaat tgacaatatg ggaactttta tagaaagtgg aaatgcattt tatgaattat
     2221 taggatattt agatatgttt ggaggtagga tatttaagtt catatatcca atattttcag
     2281 cttacgtagc atattctatt gctgatagac cagctttagt tccagggctt ataggtggtg
     2341 catttgctgg aggacttcat tttactttct ggggtgtaga aggtggagta ccatcaggat
     2401 ttataggagc tttaatatta ggtttagttg caggttatgt tactcgattt ttaaatgaaa
     2461 acataaagtt aaacaagaat ttacaagcta tgaagccaat gttcctatta ccaggtataa
     2521 caatgttagt aatattctta ttaaacttct atgctgtaga ccctgcattt ggtagagtaa
     2581 atgcatttat agcaaatata atagaatctt ttggaactgc tagtacaata gcattaactg
     2641 tagttatagc agcttgtact gcatttgact taggtgggcc agtaaacaag gcagcaggtg
     2701 ctatagctat aggtcttgca gctgatggtg tatttccatt aacagctaga gtattatcta
     2761 tagtaatacc accaatagga ttaggtttag ctactgtttt agataagatt atagttaaga
     2821 ggagagtatt tgatgataat ttacgagtgg taggaaactc ttctataata cttggactaa
     2881 tagctgtgag tgagggggcg atacctttca tgctaaaaaa tcctctaata acaataccta
     2941 taaatatatt aggtgcaata ataggctcta taacagcagt aatgttaggt gctattcaat
     3001 ggaatctatt gccagctaca tggggatggc ctttagtaga aaatttatgg gcatacctag
     3061 taggcttaat agcaggtacg ttatttatag cattagctaa tatatttata aggttcaata
     3121 ttataaaaaa acaaaataaa aatgatataa ataaaacatc ataataataa ttgggaatta
     3181 tttgggaaaa tttaactaga ataattccca tttgtacaag tggatatttg ataaaattat
     3241 ttagaaataa ttaaataaag atgattttat atggaggaag atatgaacaa tagaaaaaaa
     3301 gtatatgtag taactcactc tcattgggat agagaatggt actttactat agaagattca
     3361 aatatactat taagtgaaaa tatgccttat ctaatggatg tactcgaaaa ggatatggac
     3421 tatacttctt acacttttga cgcacagtta tctgttgttg aagagttagt aaaattatat
     3481 cctgaagaaa aagaaagact taagaggtta gtaagtgaca aaagaatatt tgtaggtcct
     3541 tggtacaccc aaacagattc tatactagta aacaaggaat ctataataag aaatcttttg
     3601 tatggtacaa gacttggaaa caagtatgga catagtatga aggtaggata tcttcctgat
     3661 atatttggac aaaatgcata tttaccatca atattcaacg gatttgatat agaatatagt
     3721 gtattacaaa ggggaatata tatagatgat ttaaagggaa atttaaattt caagtgggtt
     3781 tctcctgata ataaaagtgt tagagctaac aatatattct taggatatgg accaggtaaa
     3841 ttcttatcat ctgataatca gtatatacaa gataagttaa tacctatgct tgaaaaatta
     3901 gaaagtttaa atagggattg taacaattta cttttaccct gtggtgggga tcaggtttta
     3961 ataagaaagc aatttccaca aatcataaaa gaattaaatg aaaagcaaga taagtacaag
     4021 tttgtactat cagattatga aacttttatg aaggatactt ggactaacga ttttgagaat
     4081 aagatacatg gagagctaat agcatgtgaa aagtctagaa tacacaatac tataaagtct
     4141 caaagatatg atataaaata cttaaacact ttagtagaaa ataagattct ttatgtatta
     4201 gaaccattat ctgtaatagg taaattttct ggaataagtt ataagtctag atggttagat
     4261 attatgtgga agttactatt tgatgcacat gcacatgata gtataggtgg atgcaactca
     4321 gatgagacta atagagacat agttaaaaga cttaaaaagg ttaatgcaat gtgtgatgat
     4381 attataaata ttgttaagaa acaaataaca atggctataa gtaaatattt aaatatagat
     4441 aatatattag ttgtatttaa cactaaaaat aaatatatga atgaaataat agaggctgta
     4501 gtattcacta agaaaaaaga ctttacaata agtactatag ataaagaaat ggtagaatta
     4561 actataaata agcaagaatt tataagtggt ggtaagcaga tattagtaac tgctgaaggt
     4621 gaaaaagaag ttgaattact tggatactat agaactgaag tcagattagc taatgtaaat
     4681 gttaaaccta tgggatttac tacattagta gttaatcaag atagcaaaga aatagctgat
     4741 atgttagttg catctaatga taggagtgta gaaaatgatt tatataaatt agtattcgat
     4801 aacaataact tactattagt taataagtat accaataaaa gaatagataa tatagttcat
     4861 tttgaaaatt gtggtgacta tggagattca tatgattttt ccccattaga taacgataaa
     4921 gatatattag ctactaaagc agagctatta gaagtatcaa agagtaattt agtacaaact
     4981 atgaagttaa agcatagtat agaattaccc tttgatatag atgaaagaaa attaaataaa
     5041 aagtcagcta gattagaaat aataactaga atagaaataa gaaagggtga aaactttata
     5101 agagtttcac atgatataga aaatactgta aaagaccaca gagtaagagt tgtttacaat
     5161 acaaatgtaa aatcaaaagt atcattaagt gaccaaggat tctctgctat aacaagacaa
     5221 aattacaata ggcacttagg taattggcaa gaaagaaagt ttgctgaaaa accagtatgt
     5281 atatatggtt tagagaattt tgtagcttta agagataata ctaatacttt tgctttagta
     5341 acaaaaggta taaaagaata tgaaatatta gaagataaaa aaatagcact tactttatat
     5401 agaagtgttg gtttattggg aagagataac ttggcttgga gaccaggtag agcttctggc
     5461 ataaataaca aggtagtata tactaaagat gcacaacttc ttaacagaaa actaaagttt
     5521 gagtatgcta tatactatga taagtcagat gaagatgtaa cagaattatt tgatgttact
     5581 gataaattta taaataaata tactacttat caaaatcaag atttgaattt atttgaagaa
     5641 agattagaaa gatttgaaat acctaaatat catactataa acttatcaag ctatagttta
     5701 tttaatatag ataatgatgg tatatttatg agtgtgtgta aagaatctta tgaagaggat
     5761 ggaattatag taagactatt taatccaaca agtgaagcta aagaagtaag cataaatagc
     5821 gaatatataa aggatataaa gataactaat ttatatgaga aggagttgga tattttggac
     5881 tctaatgtac agataaaagc taaaggatat ataactctta aattattagg aggagaatac
     5941 aatgaataag acaatactgt tacaggatat agaacaaaaa cttaaaaata tatataaaga
     6001 aaagtacaag ccaaagttct ttgatatgat gaaatctact attgagaagt ttgaaggtaa
     6061 agcttttgat aaggtagatg ttatatctga aaagaatgta tatttaataa cttacggaga
     6121 tagtatatat gaagaaggta tacctactat acatactttg aagaaatttt taaacaataa
     6181 agtaggagat actataactg atgttcatat acttcctatg tttgaataca cctcagatga
     6241 tggattttct attgttgatt atatgaaaat agataaaaaa cttggaaatt gggagagtat
     6301 aaacaatttg tctaaggatt atagattaat gtatgatttt gttgcaaatc atgtatcaaa
     6361 gaatagtaaa tggtttcaag ggtacttaaa tggagaagag aagtatcaaa attattttat
     6421 atcagaagat aaaaattttg attactcaaa tgtgataaga cctagaactt ctccaataat
     6481 acatgagtat caaggaaaag atggtgtaaa gactgcttgg acgatattta gtgaagacca
     6541 aatagatata aatgttaaac attttccagt acttatagaa attactgaaa ttttacttaa
     6601 ttacgcatta aatggtgcaa gttctataag gcttgatgct ataggattct tatggaagga
     6661 gtctggtact acttgtatac atttaccaca aactcacatg ataatacaat tatggagaat
     6721 aatacttgat tattttaaga aagatactca aataataact gaaactaatg tacctcattt
     6781 agaaaatgta agttattttg gggatactac aaatgaagca catatggtat atcaatttac
     6841 attaccacca ttaacacttc atacattcac tacacatgat tctactaaac ttacaaaatg
     6901 ggcttcaact ataggtaagg tatccaataa tgctacattt tttaacttct tatcaagtca
     6961 tgatggaata ggaatgagac ctacagaggg tatacttaca gaagaggaaa ataaaagatt
     7021 agtagataag actataataa atggtggtag agtatcatac aaggataatc cagatggaac
     7081 taagtctgta tatgagttaa atataaacta tttagatgcc ttattgaata aagatgaaga
     7141 tatagatgag gatgtccaaa tacaaaagat attatcagca catgcattat tattatcatt
     7201 tataggtgta ccagctatat actatcattc tctattaggc tctaaaaact actataaagg
     7261 agtagagaaa tcaggtataa acagaagaat aaatagagaa aagttggagt ataatagaat
     7321 ttgttatgaa ttagaacatg attccagaag aaataaaata tttactcaac ttaaaaatat
     7381 gatagatata agaaaaggag agagtgcatt ctctccatat gcaaatcaaa atgtattgga
     7441 ttttggtaaa gaaatatttg cgctagaacg tgagaataaa gaaactaatg agaaggtagt
     7501 ttttataact aatgttacgc ctaatgagaa gattatagac tgtggtttta atggtataaa
     7561 tatattagaa aatattaata ttgaaggcaa gttaacttta ggtgcatata agtttgtatg
     7621 gataaaaatt aaataacttg atagttaaag ctttaataag ggaatatgtc agtttagcta
     7681 tagcatcaat gaactttgca agttcaaggc ttatattaaa tgttaataga tatatatttg
     7741 tataaaagaa atatacataa aagaatgagt aaaaattaca gataaatata aaagttggat
     7801 taaggttagt ggttatggaa tagcctttag aatctatgaa tgattagtgc taaggtacta
     7861 tcgaacagaa atattaacct tcaaatgtta tcaaaaatag ttaaggtgga gtaattaaaa
     7921 gttaattggt tcgtgataaa cacttatcga ataagtaata aataagaatt aggtttgtga
     7981 taaacactta tcgaataagt aataaataag aattaggttc atgataagca tttatcgaat
     8041 aagtaatgaa taagaattag gttcatgata agcatttatc gaataagtaa tgaataagaa
     8101 ttaggttcat gatgaacact tatcgaataa gtaatgaata agaattaggt tcatgatgaa
     8161 cacttatcga ataagtaata aataagaatt aggttcatga tgaacactta ttgaataagt
     8221 gataaataag aattaggttc atgataagat aaatacttag cgaataagta ataaataagt
     8281 agttagttta tgataactaa tttgccagtc tacgataaat agatacttag tttgtaacaa
     8341 gtaagaatat ttttttagga tttacggtat agcattttct ttatgctata ccaattttat
     8401 tttgttaaac tctataaata ttttatattt ttacgaattt atttcctttt tcatttcatc
     8461 acactttata atattgatgc tcatcctaat tgcctcatta aattactact atgaccacca
     8521 tatggctttt taatatcaag tgtaacctta tttaatatat cttatttaga tattttcgtt
     8581 ttttaagcaa atggcacagt tatatctatg agattttttg tacactttca tatgcttttt
     8641 tcaaaaaata tcgttatttt ttctatggtt ttatattata gtagtttttt gatttgataa
     8701 agatacttat tgtttaacaa tttctctaga tagaaagaat aaaaaatgaa aatattactt
     8761 gaggatagtt ttacaaatta tactttaata agtaaatgat acttaaatat agatgaaaat
     8821 aatgcttaag agtaaataca tctttgtatt gacaaaaaag tgtactaagt gtatagtgtg
     8881 tatataataa catcacactg aaagtgataa aaagggggtg ataaaaaatg aaagtgctca
     8941 tttctaacac atcagataag ccattatacc aacaaattaa agagcaaatt aaagaagcaa
     9001 tatttactga agaactgaaa gaaggagatg caatgccatc cataagaaat tttgctaatg
     9061 atttaaaagt tagtattttg actattagac gagtgtatga agaacttgaa aatgaaggct
     9121 ttcttacacg ccaagttgga ataggaacat ttatttcaac tggaaattta gaaatactaa
     9181 gagactcaaa gagacgtata gttgaacaaa aaatggtaga tatgattaag gatgcaaaag
     9241 cactaggaat tacacagata gagttacaag aaatgatgga tattctatat caggaggatt
     9301 aatatggaaa atattttaga agtttataac ttaaataaga cttataccga tttttcatta
     9361 tctaatataa gctttgcatt accagaagat tgtataactg gatttatagg tgttaatgga
     9421 gcaggaaaaa caacaacaat ccgttcaata ttggatgtcg taaagcgtga tagtggaaaa
     9481 ataaaaatct ttggtaaaga tttcgatgga aatgagaagg aattaaaaaa tcgaattgga
     9541 gttgtctttg atgattcata tttttatgag gagctgtcaa tggcagaaat gaaaagtatt
     9601 attgcacctt catatgataa ttggtgtgaa gaagattatc aacaaaatat gaagcgtttt
     9661 aatcttaacc caaaacaaaa gattgataca ctttcaaaag gaatgaaaat gaaatttgct
     9721 ttagcattag cattatcgca taaagctgag tttttaatta tggatgaacc tacaagtggt
     9781 ttagacccac taatacgtag tcagatactt gacatactaa caggatatat gaagaaatgt
     9841 ggtcgtggtg ttttcttttc cactcatata acatctgatt tagacaaaat agcagatatg
     9901 attgttatga ttgacaatgg acgtattata ttagaacgtg aaaaagatac tctattggac
     9961 gaacatagaa aagtaaaagg ggatattcaa tttctaaatg atgaaaccaa aaaattattt
    10021 ttaaatttag aaattaccgg ctatggtttt atagggatta cctcaaaaat taatgaagta
    10081 caaaaaagta tgccagatat tttaatagag cgtcctacaa ttgaagatat tatgcttgca
    10141 tatattgaaa ggagagagta attatgttga ttcatttagt taaaaaagat attctgattg
    10201 taaaaaaata tgtattgttg atattattta ttgcaatagg aatcccatta tttatattat
    10261 ggcgtttacc tccatttgca caaatattgg gatttgtact atcaattata ttttcagaat
    10321 ttatgatgtg ccagtatcta ttattaaagg agagtcaata ttcaaaagca tctgtattac
    10381 tttgttcaac tccttatcct agacgtggct tagttcaatc taagtatgtt ttatttgcaa
    10441 tggtatttat ctattgtaca ttagtttact ggattgaaaa tttacttatt ccacaaatag
    10501 gaacttttga cttaacatat attttgattg ttttattaat aagttctatt atttatggag
    10561 tttatatgcc tattcaatat aaattaggat atgaaacaac aaaattcttt ttcgtggtga
    10621 taatcatggc ttcatcattt gtattaccat taactatagc tcacaacaat tttgaaattc
    10681 catttatata tcagtatcca tccatgatag tgaatattgt attgttcctt ttaagtttaa
    10741 ttatactgtt agcttcaatg tttatttcta ttaaaatttt tagtaataag gagcttgtat
    10801 aaggtaaggg ggacttttat atgccacatt tgccagattg ggttgtttta ctttgtatct
    10861 tacttccaat agtatataaa gcttgtcaga tatttaaaag taaaaaatag agtacatatt
    10921 tatattgcca tttttattga gtaaagttag atggtatgaa aaggttactt gaaaataaag
    10981 ttacttggtg tgggagagta acatcataat attatagatt atatgtttct tgtgaatcaa
    11041 tttgtatttt ttaatagata atatccagta attttatttt ccattgatta tttttcaatt
    11101 atgagtatga ttgtgtgtat tgtataccat cttttaaggt gtttttaaaa aattatatcg
    11161 tcaaaatata aatctaagga atcctataaa taaagctttt caattttaga aaactaataa
    11221 aaagtaggaa gttaataaaa agaagctgtc tcaaaataga gataagcttc tttttcattg
    11281 ctgaaatgac gaaaatataa ttgatttaat ataaaaaaga gtgctttatg aactatttat
    11341 tttgaacagt agatacactt gcactcaatt tagttactcc taagctttat gaactaaaag
    11401 tttattttga agcctatttt tattaaaaga gattttattt ttctaaataa tcatctatat
    11461 acagtattgc gtttctttta tttattcata atttattttc ttattaaagt ttttctatat
    11521 tcatattttc catattactt tacttttaat tttatacgaa ttatcaaaaa taaaatagtt
    11581 attagaaaaa aataaaagta attgcaaagc ttgcaattac ttacttctat attaattact
    11641 ataccataaa ataacaaaaa aatcaagtcg agtaatcctt caacttaagt gatatactag
    11701 gtattaataa tttttaatta ataaaatata actagaggaa aatctacatt ttctcaattt
    11761 ctttaattaa attgacctct aattaagtaa ttataaggag aaaaattatg aatttagcaa
    11821 agggattaga tgttttaaaa atttcatcaa atgtattcgg tgaagataaa gttatgtata
    11881 ttcctgtaat ctacacagaa gatgatgcaa cacttattga cacaggtctt ccgggtcaag
    11941 gtgatttaat aattgatgct cttaataaaa gtaacacgag ttttgataag ttaaaaaata
    12001 taataattac tcatcatgat atagaccata taggaaacat aaattatcta agagaaaaat
    12061 ctaagaataa tatcaaagta tatgcataca aaagtgaagt cagctatata acaggagaag
    12121 aaactccttt taaactttat atgttggaac aaatggtaga taaaattgat gataagatgc
    12181 ttagtatgct aaatgttatg ggacttggat ttaaatcatc atatactaaa gtagatgtgt
    12241 cattagataa ccatgaaaaa ttgaatctag gtgaggaaat agaagttata catacaggag
    12301 gtcacactag aggtcacata tgtctttatt taaaagagtc taaagtactt attgctggag
    12361 atttgttaca ggtagaaaat ggagaactta agcctgttga tgtaatgcac agcaacaaac
    12421 aagagttaaa agatgctatt aaaaatataa gtaattatga tatagaaacg atagttttta
    12481 gtcatggtgg attatatcaa agaaacataa ttggaacatt gaaaaaccta attattgagt
    12541 agtactatat gatttacaat aatttcttaa attacttaaa attatatcta aagatgtaga
    12601 atcaaaatta aatataagta ataagaattt tgctttaaaa ttatatttaa gtgttaaggg
    12661 tgatacgtag tattacaagt attttagaat atagtttttt atagtaattt taaggttcta
    12721 caatacttca gttgaaaaat atttaacagc atagtataat gggaaagaaa atactttaaa
    12781 taaactttag tatacaaagt gaagaaaaat atttaaatta tatataatat tgtagagtga
    12841 gtaaaaatat ttaaatcatg tatcataatg caaagtaaag aaaatcattt aaattatata
    12901 tcataaaaat aatatgaaat attgaacact cataatatta gaggctttac tattttaatt
    12961 gataggttat attatttata acattaaaaa tacaatttac ataaaataga catatgaaaa
    13021 ataaaaaatt atataataat tatgggaatg aggttctccc ttggatgata atatcctaaa
    13081 ctgccaatga gctgatgact tctatgatat catagatgcc accagctttt tttgttgttt
    13141 aaatcgataa aatggaggtg aattacttac taaattttta ttttttaaat ttgaagaaat
    13201 atttaattaa tagaaaaaat agtagattat atgttaatta ttttattaag aatgggctaa
    13261 gagtatacct atatcctatc aatctaaaaa acaattatga tagtcattac attgactaca
    13321 gttgatgcaa taaaactttc aaaaaatctt atgatgcaaa tagaaaggaa ttaaaatgat
    13381 agaaaaattt aaaactttaa tatggattaa aatgataata agaattaaaa gcgtttataa
    13441 aacatatggt ggactatttt ttttagggct cataggtata ccagttggtg caattatagg
    13501 tcttatagac accatatttg gaacggttct tctaaaagtt acagatattc gagaaacata
    13561 tccaatgtat ctgataccat ttttagcagt agtaggagtt gttattgctt attgctattt
    13621 taaatttgga ggaaaaagta gtaaaggaat gaatctaata tttgaggtag ggcatggaga
    13681 agaagaaatt attcctttaa gattggttcc atttataata tcaggtacat ggttaaccca
    13741 tttatttggt ggtagtgcag gacgagaagg agttgctgtt cagattggtg caactttttc
    13801 acattgggta ggtaaaaggt tgcctataaa aaatgcttct agtatttttc tagtaactgg
    13861 tatggcagct ggttttgcag gattatttga gacaccaata gcagcaatac tatttgctat
    13921 ggaggttttg gtagctggtt cactggagta tcaatcgctt tttcctgcgt ttactgcttc
    13981 ttttacagca agtgcagtat caaaagcttt agggttagag aaattttctt ttgcactttc
    14041 ttctaaagtt gtatttgact tgtcaatatt ttggaagctt attgttttag gaattatttt
    14101 tggaatggtt ggtggagcat ttgcatggtg tctgaaattg tcaaaaagaa aaataggtaa
    14161 tagattaaaa aatcctatga taagaattgc tattattgga gtatgtctta gtgtattatt
    14221 ccttttattt tataaaggaa gatattcagg tttagggaca aatttaattc aaaatagttt
    14281 ctatggtgga gaaatttatt catttgactg gctgttaaag tttatactta caatactgac
    14341 tttgtcagca ggttttcaag gaggagaggt tacacctttg ttttccattg gagctagtct
    14401 tggtgtattg ttagcaggat tttttaattt gccaattgaa ttagttgcag cgcttggcta
    14461 tgctagtgtc tttggtagtg ctacaaatac tttttttgca cctgtattta taggggcaga
    14521 agtttttggg tacagctatt taccttattt ctttgtagta tgcgctattt catatatatt
    14581 taatatggat aaatctattt attcactaca aaaaatttca acaaaacaat aaataaaaca
    14641 acataatgta tgattagaag agaaagaata ttagagaaag gatagggtag gaatatgttg
    14701 acaagtttag ctttgatttt tttattagga atggcctcgg gaggtatttt taagaggata
    14761 aaattaccta acttattggg aatgttactt acaggaatta tattaggtcc atatgtttta
    14821 aatttgattg ataattcaat actagatatt tcttctgacc ttagaaagat tgcattgatt
    14881 attatattaa caagggctgg attatcttta gatataaacg atttgaagaa agtgggaaga
    14941 cctgctgtac ttatgtgctt tattccagct acttttgaaa ttattggaat gatagtttta
    15001 gcaccaaaat tactaggcgt ttctatattg gaagcagcag tcatgggtgc agtagtagga
    15061 gcagtttctc ctgcaattat tgtacctaaa atgcttaaat taatggaaga aggttatggt
    15121 acagaaaaaa gtattccaca gatgctttta gcaggaactt ctatagacga tattttcgta
    15181 attgtgatgt ttacagtatt cacaggattg gcacaaggaa atagtatatc agctattagt
    15241 tttttacaaa ttccagtatc aattatatta ggtgtaatag ctggagcagt gattggaatc
    15301 tgtttagcag tattttttaa gaaggttcat atgagagatt ctgctaaagg ggtgttgctt
    15361 ttaagtatct catttttaat gataagttta gagactgctt tagagggtat tgtgccattt
    15421 tctggattat tagcagttat gagtattggg attttccttc agataaaata tcgtgtagtt
    15481 gcaagaagat tgtctataaa gtattcgaaa ctatgggttg gtgctgaaat tctcttgttt
    15541 gttttggtag gggcaacagt agatataagt tatgcattta aagcaggaat tggagctgta
    15601 attttaatat ttggagttct tttattccgt atggtaggag tattcttctg ccttattaag
    15661 acaaatttga caataaagga aagattgttc tgtatgattg gctatattcc aaaggctaca
    15721 gtacaggcag caataggagg tgtaccactt gcaatgggga tggcatctgg acaacttatt
    15781 ttgacgttag cagttctagc tatacttata actgcacctc ttggagcttt tggaattgat
    15841 gtaacttata aaaaattatt aacttctgta aaaggcgaga ataagtaaaa ataaagatta
    15901 ttttaagtgg taagttatat tttggaaaag gattatacta ttgaatacaa tataaaaaaa
    15961 tcatttggga gattaagaag gtagaagtta agaacagatt ttaaggtatg ttatgattat
    16021 tagaattgat tttgtgaaat tattattgta atatttaatt atatagataa agaagcggag
    16081 tgataactat gtgtttaaaa aaattatcaa aattagagtt agttattatg aaatttattt
    16141 ggaatttaga tataaaaacc aattcatatg agattattga ttatatgaaa gaagaacata
    16201 atttaccaga aaaagtagct ttaaaaactt tatcaaaact atcaaagaaa agattcttat
    16261 atgttcaaga aacaggcaag tgtatgtatt atacagttgc aataaaagaa aaggcatatt
    16321 tagagtttat atcaagaaat gtgcaaaatc ttttgaaaaa taattttata aggaatttat
    16381 tagtatcatt tcacgaagag gagctaacag aggaaaagat aaagtcgtta gaaaactggg
    16441 tagtaaattg ggaagaagct tatgtataaa tgttattatt tttattacaa tttagataat
    16501 tttggttttg taagctctgc aactattttt agatggttgc agagtttatt tttatttttt
    16561 attcccttaa gcaatactta aaagtaaacg catttatatt tactaacttc aattacttct
    16621 aatacttaaa ttacttttta aaaatatttt gatatgtcat ctctatagta atctagaact
    16681 tgtttctatt ctttatataa agtttttatg aatgatatat ataatattcg ttcatttgat
    16741 taaaaataac aaaatattaa ataattctac tctgatagtt tgttaaaaaa ataataaaaa
    16801 atattaataa acaaaaaaaa aattatctta agagaggaga atgttctaaa atataaaaag
    16861 gtttctagat ttcataaaag atactatttt agtcttgaaa atatttagtt tgaaaagat
//
LOCUS       DAEMHW010000074        16740 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEMHW010000074 DAEMHW010000000
VERSION     DAEMHW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.074"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(498..908)
                     /locus_tag="KPK67_002029"
     CDS             complement(498..908)
                     /locus_tag="KPK67_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2458997.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYVAID"
     gene            complement(1124..2506)
                     /locus_tag="KPK67_002030"
     CDS             complement(1124..2506)
                     /locus_tag="KPK67_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG2458998.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIEQSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2517..2876)
                     /locus_tag="KPK67_002031"
     CDS             complement(2517..2876)
                     /locus_tag="KPK67_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2458999.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(3081..3860)
                     /locus_tag="KPK67_002032"
     CDS             complement(3081..3860)
                     /locus_tag="KPK67_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBG2459000.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
     gene            complement(4321..4932)
                     /locus_tag="KPK67_002033"
     CDS             complement(4321..4932)
                     /locus_tag="KPK67_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2459001.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
     gene            complement(5024..6013)
                     /locus_tag="KPK67_002034"
     CDS             complement(5024..6013)
                     /locus_tag="KPK67_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBG2459002.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            complement(6080..6943)
                     /locus_tag="KPK67_002035"
     CDS             complement(6080..6943)
                     /locus_tag="KPK67_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBG2459003.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            complement(7058..7645)
                     /locus_tag="KPK67_002036"
     CDS             complement(7058..7645)
                     /locus_tag="KPK67_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBG2459004.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRYINILK"
     gene            complement(7685..8488)
                     /locus_tag="KPK67_002037"
     CDS             complement(7685..8488)
                     /locus_tag="KPK67_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBG2459005.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            complement(8524..8964)
                     /locus_tag="KPK67_002038"
     CDS             complement(8524..8964)
                     /locus_tag="KPK67_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBG2459006.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            complement(8977..11079)
                     /locus_tag="KPK67_002039"
     CDS             complement(8977..11079)
                     /locus_tag="KPK67_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBG2459007.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            complement(11292..12995)
                     /locus_tag="KPK67_002040"
     CDS             complement(11292..12995)
                     /locus_tag="KPK67_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBG2459008.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGIRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            complement(13021..13980)
                     /locus_tag="KPK67_002041"
     CDS             complement(13021..13980)
                     /locus_tag="KPK67_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2459009.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            complement(14116..14634)
                     /locus_tag="KPK67_002042"
     CDS             complement(14116..14634)
                     /locus_tag="KPK67_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBG2459010.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKIIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            complement(14677..15162)
                     /locus_tag="KPK67_002043"
     CDS             complement(14677..15162)
                     /locus_tag="KPK67_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBG2459011.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            complement(15159..15776)
                     /locus_tag="KPK67_002044"
     CDS             complement(15159..15776)
                     /locus_tag="KPK67_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBG2459012.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            complement(15841..16203)
                     /locus_tag="KPK67_002045"
     CDS             complement(15841..16203)
                     /locus_tag="KPK67_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG2459013.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
BASE COUNT         5397 a         3005 c         1617 g         6721 t
ORIGIN      
        1 tttgattttt ttaaacaaat cttaaaaatc ctaaaatgct aattttcttt aacttttaat
       61 gaatctaata gataacattt aattttcgtt atatttttca gaatatttct attattattt
      121 ttaagaaaaa aaaataatgt taacatttct taacatacat taaaaaatgt taacattata
      181 aattgggttt aatactttta ctttgactcg atttactttt attctacttc ttactttatt
      241 tttaacttag actttatttt tttattactt atttgagtct tatttatttt attatcactc
      301 catatgttca attacatttg tctaaattct ttgatacttt tattacataa ttttaacaag
      361 cttaagtgtt atttgggtag tgcagttgat aattatttaa ataatcatca actgcatgtt
      421 tatatcatta tagtagctta atgtatttaa tcatacttac ttaggatttt gaaatatctt
      481 agagattata tttaacatta gtcaattgct acataattgt aagcaattga aatttgagac
      541 ccatcttcat tttctatagt gataggcttc ataattttta atccttcagt ttttatcttc
      601 tctaattctt gttccatttt cttaattgtt tcctcaaggt ctttttgact tacatctttg
      661 actgcattag ggtctttagc atagtctttt aattcttgaa tatgtttagc atactcttca
      721 taagtataat gctcaacacc atacttttcc aattcagtag ggtcagttat tcctgatttt
      781 attagagatt caagtttagg taaagcaata ttagatgaat catttccatt tgcaaatact
      841 gctccagttc ctcctagtac cattgcagat accatcattg cacttataag ggattttttt
      901 attttcataa ccaatcttcc tttcttagag atttatttgg gtagtgtagt tcgaaatgac
      961 tattgcaata gttatcaacc acatccttag actatcacag tagtcatatt tatgtcaagc
     1021 ctttttaaaa aacttttcaa taaaaaatac tatctcaaaa caaaattaat tattttgaga
     1081 tagtatttta attctaaatc ttttatatta ctaagattta tggttactct atccatgttc
     1141 tatattgttc tttattaact gctaacaaat tcccttttct tgatatgaca aagcgattat
     1201 cattatagcc actttgctca attgaaactt ctcctgaatc agtataaagt acatccttac
     1261 tattactaga tttatttaca tacgtttctt cactattctc agaattagtt actttgctca
     1321 tagatgattc ttcatgctct ctaaattcaa ttacagatac ttttgccgta ctaaaatgag
     1381 tctctccatc acttttagct ttactaacaa gaatatcaga ttctactttg ggagaatata
     1441 aatcagttga tttactactt attgaaaatc caattttatc actttgttca ttttttgtta
     1501 aaccaaaaga ttcaacctca ttaatctctt tattttctgt tgcaaaaaca agacctgtcc
     1561 cacaaactaa tacagttgaa actaaaaaag taacataagt tgatttttta aatttcataa
     1621 tattaactat tctcttttct atttttgtct ttccaaatcc actacatatt gtcattgaaa
     1681 atggattatt ctcctctgct agacttatta gtgtggttgc ataatcagcc ctatattctt
     1741 caccaagtat actcataacc ttttcatcac aactaaattc tatgtcttta tttagtaaaa
     1801 aaaacatcac ccaaactaag ggattaaacc aatggacaac aagagctaca agcataaata
     1861 actttaacag attatcaaag ctattaatat gaaccatttc atgagttaaa acaaagtcta
     1921 aagtttgatt attagcataa ttcatttgct ttggcaaaat aattcgtggt tttaaaatac
     1981 cagatgtaag tggtgtccta gtaaattcag atatgcaaac aataaatctt ctaaaacggc
     2041 tattcttaca catccatttt ttaatgaact catgattttt tatatgaatt gaagttttaa
     2101 aaagattaat tattttaaaa tatctaaagc ataaaagtat gctaaacata atagtaacta
     2161 taaaagacaa aatgcccaac atttccaaca tcataatatt ttcaatatta gacattattt
     2221 caaattcgtc agaattgttt ttacctgtag agtcactagc tttcttaaga gtataatttt
     2281 ctgttgatgt attacccaat tccacatcaa aagtcgattc aattatacta taaatactaa
     2341 aaccagaatt taaacttact ggtattagaa gtttaaataa aacaatcaac cacaataata
     2401 caaatacctt tttaggtaat tttttaaata aacatgctct tataaaaata actattaaaa
     2461 gtaatactcc agaagtaaaa ctcatttcta acaatttaat gaacataaat cttacctcac
     2521 tttaattttt caacaatttg tttaagtttt gtaatctcat cacttgaaag attttcttta
     2581 ttaataaaag ctgaaagaaa tttttctgaa gaccctccaa acattttatt aattaaagtt
     2641 tctgtttcct gtttttgtac ttcctctttg cttattaaag ctttacaaac aaaattcggc
     2701 tcaattctct ctattgcacc ttttgacact aattttttta taacggtata cgtagtgttt
     2761 ctattccatc ctgtttcttc ttttaaaatt tttactaact ctccagcctt gctttctcct
     2821 ctctcccata gaacttccat tactttaagt tctgaatcaa ataattttat ctccatataa
     2881 aatcgctcct caaatatagt tatcacaata gtcattacta taataaaaaa ctatcatagt
     2941 agtctatact atgtcaatca tttatttttt aataataaaa actgtctttt taaaaaattc
     3001 ttataaaaga cagttttatc aatgttatac tttaatgttt aattaaagcc atattttatt
     3061 taaatatata actatctatc ttagtacata gtataagatt tttctttatc taatagtata
     3121 tcctctacat ctgggttttc ttttactaat tgtctataaa aattacaatt agtaacacct
     3181 acatatggaa ttagccatag tagacctatt ccaagagtag ctattgaaag tatataccat
     3241 cctataaaac taaagtttag tacaaataat ttaagtacat ttcctttcat catatctaaa
     3301 ctagcaccca ttgatttaaa tacacccata ttatccttgt cttcacaaat taagaaagta
     3361 gcttgagaaa catataatag caatatataa actggtaaca taagaacaag tattataaga
     3421 actagagtta ttccagtaga aataatagtc cctattccaa cattatcact tgataaatat
     3481 tcaattgctc cactagtaag taaaactcca aatgataaaa acgctcctat aattgtgatt
     3541 ataaaagaat aaatataaac aataattgat tcaaataaat aaaaaccaaa acttcttaaa
     3601 taaattctcc cacttacaaa gatatctcct atcttgactt tttcatcttt agtaattttc
     3661 aagaacatac atgtaaatcc tgctgaaacg caaaaagcta ttacagccac tacaatagta
     3721 gtcaatggtt caaaaggtaa tcctacaatt gatattaata ctatcaatgc aatatatact
     3781 gccatagaaa ttgcacatat cccccagttt ccagataact gagctttaga tactcgtttt
     3841 aattcttgcc ttgataccat aaaaaacacc cctttcattt tataaaaaaa cttttaaaaa
     3901 tataaataat cttttaacgt ataatattaa tatatcatat tttcgactat ataagataca
     3961 attattcacc atgttttatc aagatatgcc ataattgtat tactagtttt atatagtcaa
     4021 cattaccaaa tacatcaaat aaatttgatt aattattcaa caagtaataa aataatttct
     4081 attaattata ctttatttag taaacaaatt atttaataaa aaaattactt aatacagtaa
     4141 attaattggt aagataaatt atttattatt agtaaagtaa attatttact atttatcaag
     4201 ataaattatt tataaaataa atcatttata aaatataatt tatactttga aaataaaata
     4261 aactatcttc caaacatttt attatatgct taaaagatag ttttgtgtct ttaaaataaa
     4321 ttaaattgta tatattcctt cacataactt ttgtatatta ctaggtggtt gccatttacc
     4381 atctttttta gtatatgtaa tcatagttgt aacttcctta gttggtttct tattgtaaag
     4441 gtctataatc atttttgtca tatcatcttg aacttctttt tcttttttat cgtaattctt
     4501 ttcatcagtt acataagatt ttactaaagt gttcgcttca ctcattactt tactagtatc
     4561 taaagcttta cttacaactt tcaaagttat cttatcctta ctagaagatt ccactgttac
     4621 tgtcttttca acaccttgaa ttaatttaac aattgtttcg tatatctttt cagcattttc
     4681 tttaccataa agaggtgtca attgagcaat cattgaaacc ttgtccttgc tgttataagt
     4741 ttgaatctgt ttatattcag cttctgttaa tgcatcagaa attccttttt catcatactt
     4801 atacatttct aatgcagcta aattagcttc aaaattcgac tttacttttt cttctgcatt
     4861 agaacaccct atagttagaa ctaaaactag agccataatc gtagataata ttaacttttt
     4921 taccattttc atacaaacat cctccctcaa ttaaaataat aaaaaattta tttatcaaca
     4981 ttataacata aatttgtcta actatagaat tattttgaat attttaccac ttccagtcta
     5041 ttccaatacc gttttctttt gctttatcgt atattctttt tgctgttacg acatcttgta
     5101 cacctatccc aacagtttta aatacaatta tctcatcatc atcttctctt ccaggaatag
     5161 ttccgtttat aacattccct aaatcaccac taaaatcatc tttacttatc ttaccttctt
     5221 ttaatggaat taatatatct cctgactcag ataaaactgc atctgttgat tcaaaaaata
     5281 tcttagatgc tctagttaaa cagtttgggc acaactcttg catatctggc atatatgaac
     5341 ctactgcact tattaaagct ccttttttca ctaagtttcc attaagtact ggttgttttg
     5401 atggagtagc taaaactatc acatctgcgt tatctatggc ttcatcagat gattcaactg
     5461 caactatttt agtattatat ttacttaatt ttaagttcat ttcttctgca aatttttctc
     5521 tactttcctt gtttctacta aaaactctta cctcatccaa atttctagct tctatcatgg
     5581 cttcaagttg tggctcacct tgtcctccta gacctatcaa agctccaatc ttagaatctt
     5641 ttttagccaa tacatctata gctgctccag aggctgctcc agtacgaatc tgagttacat
     5701 aagtaccatc caatactgca ctaacttctc cagtttctcc atctaataat agtactgttg
     5761 ctggaattac tggtttgcct ttttgagcat ttttaggaaa tacagataca atttttattc
     5821 ccccacagcc taattcttct acgtatcctg gcataaataa catactagca tcttgacttg
     5881 gaacagatat attggttctc aaaggattta cactctttcc ttcacaataa attctaaaag
     5941 cttccttatc agcttcaatt gcatctctca ttgtaaatac tttttttatg tcgtcttttt
     6001 ttaaaagtag catttttata cccccaattt taataagtct ctatgctaaa tattatagca
     6061 taacatttaa tttaatcttc tatttcaaat caaataaatc tacatactct ggttttagca
     6121 tagaacttat caacgcttta tttattgaaa attctggtat tcctgctgca tatggagtta
     6181 tatcatataa atcaaaatat acaactagat tctcatcttg caagtaaaag ttttgattct
     6241 cttttatacc tttaaaatta tatattccga tattttcttc atcctttttt tcaagttcag
     6301 ctatttgtct tttaatttct tcatctataa cttctttata tttagtatta tccttaaaca
     6361 agtccattaa ttctaaaaat tttccagttc tcatatccac attatatgct atatcttgat
     6421 aaaatccatg tgctccacca ctataagtat aatatctcac ttttatacta agagcagaat
     6481 cagtattctt tttaagttca aagtctacat ttgcaacaaa cttatttttc tcgtcaggat
     6541 tatccttaag atactgcttt gcttctttat atgatttatt ataaaattca aatatgtcat
     6601 tttttatctt atcatttgta gacctttcaa caattttatt tgaatttgtt attacaggca
     6661 tgtttatagt actttctaaa tactcatcat ttttcactat tgttttactt cctattgtac
     6721 ttactgataa agtttgttta ctatttgtat taccctttgg taatgactgg ttactcataa
     6781 cagtcactgc ttccttatta ctatttaaat taattgaagc atctttcctt aaattattat
     6841 atttatagtc atgtttaatt gccaaaacag ccattatagt caatgttaaa acaaatacat
     6901 atcttaaaac tttagctttt gtatcgactt taaaaaattt catattatca ttttcctcct
     6961 aaatataaat ctctcttatt atatttggag tttctagggt tttttatcca tataatttaa
     7021 taaaagatta atttttattt ataaaaaatt aatcttttta ttttaaaata ttaatatatc
     7081 ttatcaaaca gtttgaaatt tcaccaagag gtaattgtaa cataacagaa cctctatcaa
     7141 aagctaccat tggcattcca tatactatac aagaattttc atcctgtcca attgtaaatg
     7201 aaccattttc tttcattttt aaaagtccac ttgctccatc ataacccata ccggtaagta
     7261 ttattcctat tgaattttta cctgctactt ctgctactga atcaaacaaa acgtctactg
     7321 atggacagtg accacttatt ctttctcctt tttcacaagt tacataatat ccttgtttat
     7381 ccgtacacaa tttcatctgg taacccccag gtgcaattaa cacatttcct tgttcgactc
     7441 tatcattatt cttagcttct cttacattca taatacactg cttgtcaagc cgttgtgcat
     7501 acatataagt aaatatagct ggcatatgtt gaactacaac aatccctggt gttgattttg
     7561 gtaggtcttt tataatttca agtattgctt ctgtaccacc tacagatgcg cctatagcaa
     7621 taaccatatt attaaaacgt tccacattac cgcctccaaa aaatcatata tcaaaaatac
     7681 ctttttatcg tttatatata gcaggtttta catatttgta tttagtacta cttctgttga
     7741 ttacttctgc atgtcctaca aacaaaaaac tatctttttt ggtagcatta taaaatttat
     7801 ttataagttc ttctcttgtt tttaaatcaa aataaatcat aacatttcta caaaaaatca
     7861 aatcaaaatg ttgatatgag aaatcattca ttaaattaaa cggcttaaaa attacacttt
     7921 ttctaatctt atcacaaatt ttaaatgtac catccttgtt gtctataaaa tacttcttct
     7981 tccataactc aggaacattg ttaacatttg attctatgta tatacctctt ttagcttttt
     8041 caagtgagtt agttgaaata tctgtggcta tgattttaat gttccacaac tctttattat
     8101 atccaaaata atcatccaaa gtcatagcta ttgaatacgc ttcttctcca ctagaacatc
     8161 ctgcactcca tatatttatg tttcttattt tattattttc ttcccaaaaa ggaagtatac
     8221 tattctgtat aaactcaaaa tgttgaggtt ctctcataaa aaatgtgtgg ttagtactca
     8281 atctatttat taggtttata gcttcatttc ctgtcttatc tttaaataat agattcatat
     8341 attgactaaa agaactaagt tttttctcta tcattgtatt gtataagcga ccttcaatta
     8401 acgctctttt tttacttaaa tcaataccat attctttttt tacatggttt acaagaacta
     8461 taaattcttc atctgtgagt tttatcatgc aaaaacttcc tcctgctaat ttatcagaac
     8521 taactatatg ctattaatca aaaactttcg aatatctaaa attaatatag aattatcttc
     8581 aactttcgca atctctttta tatctttatt aatattagtg ttgttagaat tagtcgtctc
     8641 tagtaaattt agattttcta tatgaataac atcaacaact ttatctacaa taattccaac
     8701 ttgatactca tcaacctttg taattattat acaagttctc tcattataat cagctttagg
     8761 aagattgtac tttatatgca tattaataac tggtattatt cttcctctca aattaataac
     8821 tccacttata tatgttggaa gacatggtac atacgttatt gattgaaatc ttatggtttc
     8881 aatcacattt tctaattcta ttccataaat aacatcattg acataaaatg ttaatacttg
     8941 tttaataatt tcatctttag ccatacttta aatcccctaa tatatttcta taatgctatc
     9001 tgcatctata attaaactta tacttccatc cccaagtatt gtacatcccg ataaaccctt
     9061 attttttata ttaaatcggt taaaataaac tggaaatggt ttcacaacaa cttgctgatt
     9121 acctattatt tcatcaacaa aaatgcatac tgctttatgc tcattttcaa taattatcat
     9181 attaccattc atcagattgg ttacttttgt ttgaatatta tatattttat gtaaccttat
     9241 aatgggaata catgctccat atattgtaat taactctgtc ttatttcctt ctgtaattat
     9301 ttgatttaag tctgtaagtt taaatgttct tcttatggat gatattggta aagtaaatag
     9361 tgaatctcca actttaaact tcataccctc tactatagaa agagttaatg gtatttttat
     9421 agtaaaggtt gtcccttcat cctgtttact ttttacttca attgaacctc caactttttc
     9481 aatactttta cgaacaatgt ccatgcctac tcctcttcca gaaaattcag ttatagattc
     9541 ttttgtagaa aaacctggag tcataatcaa agaattgacc tcgtcatcag tatactcatt
     9601 actttgatgt ttaagaagac cttgttcctt agcttttgcc aaaatcttat ctctcataat
     9661 acctctacca tcatctgaaa tacttatcac agcttctccg cctagatttt cagcagataa
     9721 aactatcttt cccacttctt ctttaccttt cgctatacgc tcctcaatag gctctattgc
     9781 atggtccata gcatttctta taaggtgcat aaaaggttct gctaaattat caattacact
     9841 cttatctacc tctgtctctg ctcctatagt tattaactct acatttttat ctaatttttt
     9901 attcatatct cgtacaattc tatgcatttt ttgaaataca ccagcaattt gaatcatacg
     9961 catagacatt actatatctt gcaattcatc tgtaagcttg tgtaattgtc ttgttgcctt
    10021 attgaaatta tctatatcaa cacctgtaga atttacgcta tttacaacca ttgattccat
    10081 aattacaatt tctccaacta tattttgaag taagtccaat ttttctaaat ttacactcat
    10141 aatactctgc ttacttattg gtaaatgttc attttcttca cttagactat ctttttccat
    10201 atcagttttt tcatttaaaa catcttcttg tatctcatct tctgtatagt tattttgata
    10261 acttttactt actttattat tatcttttaa tttattttct actgcatcat tgtttgatgt
    10321 atttttcatt tctatttctt ttttactaat atttatgtat tcttttacat agagattatc
    10381 ttctatagtt ttataaacta ttgccttatc ctctttagct acaaattgta tataaaaccc
    10441 ataactttta atatactctg tagactctgc atttgtttct acatcttttg gataaaaatc
    10501 tataaattca cagtaatctc taagttcaaa tacaagcatg aaagctctca cattttccat
    10561 catgcaacct tgttcaaaga ttatttttac tacatcagag tcttctttgt attcaaaatt
    10621 tgaatctatt acttcttttt cgtctgtact ttctaaattt gcattatcct ttgatttcat
    10681 agaacttgat aattccaaag tactcacata ctcttcaatt ccttcaataa aattagagta
    10741 atcagaagat atacaacctc ttgattgtaa ctcatctata tctcttctta taaaatctaa
    10801 tgattgaaag actaaatcaa ataataggtc attgtctaaa acttctattt taccttcacg
    10861 tatagcataa aacaagtctt ctacactatg tgctaaagca gatatattgt ctaaccccat
    10921 catagatgct gaacctttta tcgtatgcat tattctaaaa gtttcattaa tgttgttatg
    10981 agtaaatact ttctcttttt ctatttgaat cataatttca tctaattgtt cgagtaataa
    11041 acttgtctca aatataaaca tatctaacat ttgtctcata ctcggttcaa tataattcac
    11101 atttatcacc tacgacctac aaataatttt ctattatata tgcatctcat aatttaaatc
    11161 cttgatgatt ctcttatctc actatatgta cctcataatt taaatacaca atcattctct
    11221 tatttcaata tatatacatc tcataattta aatacatgat aattctctta cttcaatatt
    11281 cacctcataa tttaaaccca tgatgattct cttacttcac tttcctttag atgaaatttt
    11341 tctactaatt cttttaaaat ttgagcttga gcagacatct cttctgaagc tgctgcactt
    11401 tcctctgctg ttgctgagtt catttggact actatggaaa tttgctctat tccagataaa
    11461 atctgtgtaa ttgctacttc ttgactatgt acagccgaag aaataccatt aatataatta
    11521 gtagtttttt ctacaccatt tactaccaag tccaatgcat ttgcagtctc tttagcaatt
    11581 ctgataccat tttctactgt tttaatagct tcttctatga gaatagctgt attttgagct
    11641 gcttttgcac ttttacccgc caaagaccta acttcatctg ctacaactgc aaatcctcta
    11701 ccatattgac ctgctcttgc tgcttcaaca gctgcattta atgcaagaat attagtttgg
    11761 aatgctatct catctattgt tttgattatt tttgatattt cactcgattt ttcacttatt
    11821 tcacccattg atgtcatcat atcatccata catctacttc caaaaccaac ttgtgatgca
    11881 gattggtcag atacctcaca tgcaagacta gcactatctg cacacttctt aattccatct
    11941 gatatctcat taatcgtgga agctaattct tctactgaac tagcttgttc agtagcaccc
    12001 tgtgaaagag cttgtgcacc tgctgcaact tgttctgaag cacttgcaac ttgctctgct
    12061 gataaattta tttcagaaaa atcaccatta aaagagttta ttatactttc taaagatatt
    12121 ttaattgatt taaaatcacc tataaattct tggtctatct ctactcttaa atctcctttt
    12181 gcaatactag ttaatgtact gtctataagg tctatgtaat ttaaaagcat actttgcatc
    12241 tttttgacag aactcactaa tagtcctatt tcagatttac tttcttcaac gtctatactt
    12301 gttcttaagt ccccagatgc aattcttgaa aagtgttttt ctactttatt aataggaatc
    12361 actattttta attttattac tgtatattct attattgtca atattattaa aagggaaagt
    12421 ataaatattg ttcttaatat tagactttgt aattcttttt ttacttctac aattttggaa
    12481 tttgaacggg taactatatc ctcttgaaat tcatttatta aagaatccat tttctcttgg
    12541 gagtttatat agtcactacc aaaaattata ctttgagctt tactcttatc attactttcg
    12601 actgccttta ttgcactttg ttctgttggt actaaagaat ctgatatttc ttttgcttct
    12661 aagattttat ttgactcgct ctttgtaaga ccaatttttt ctaatttgtt taaagctgat
    12721 tctcttgttt ttccatataa ttctttttca taaatatctt tattcttttt ttctcctgta
    12781 aatacatatc ttcttaatac atcacttatc tctctagttg tatcttttat atactgactt
    12841 tgagtcctca aattattcat atcattaata cttttttcta ctttttcaaa tgctttataa
    12901 gatgaaatag aagaaaaaat ccctgctaga acagccaata ctataataat caacaataca
    12961 attgctaatt tagaaagctt tacttctctt ttcatatctg tactccttca taaagtaagt
    13021 ttatatcttt tttaatcctt tcattgtact cactacgcta caatcttttc ctaagttttt
    13081 accctcatac attgctttat ctgctaagga tatgttttct tctaatgata tatttgagtt
    13141 tacttctact actcctaatg taatagtgca ctttatttta tgttctttat agttgaaatc
    13201 aaactccttt atctctttac ggattctttc tgaaagtatc ttagcacctt ctattggagt
    13261 atttgtgagc attatcaaaa actcttctcc accccatctt gatacagtat caatttgtct
    13321 acaattcttt tttattatac ttgatattac tgttaacacg aaatcccctg cttcatgccc
    13381 ataagtatca ttgataacct taaagtcatc aatatcacca aggataaggg atattgatgt
    13441 ttcactatat ttattaattc ttctttgctc cataagctga ttaattatat gtcgccgatt
    13501 gtaaagacct gtaagataat ctcttacaac gagtctattc aatttttcaa gagaagattc
    13561 cagttcatta tttatctttt ttagtttatc ttgagatttc tttacttcaa gatgagctat
    13621 aacacgagct tttaattctg tcatactaaa aggtttcacc atatagtcaa ttgctcctac
    13681 accaaatcct tttactatgt ctgtatctgt attttttgaa gttataaaaa ttataggtat
    13741 atctgctgta cgagcattag attttacttc cttacaaaac tcaaaaccag agttatctgg
    13801 taaaattata tcaagcaaaa taatgtctgg tataaattct tcaataaatt cttttgcctc
    13861 tattgcatta aatgcttttt taaccttaaa attatctttt gtaagtgttt tatctattaa
    13921 gtcacacatc agaacactat catcaacgac taaaattgtt ccctgttgct tatttttcat
    13981 atacaaacct ctcttaaaca taatatattc aaattttaat gatttaaaat ttttatactt
    14041 aataattata ccactatatt cttaaatgtt ctcatcacaa tctttttata ataacactcc
    14101 aactacatcc ctatgttaca tgatagtgca atcaaaccca ttgattaatt catctattaa
    14161 aacttcatct ttatctgtca aaagtttctc taaatctagt aaagtaatta ttttaccttc
    14221 taattctcca actccactta caaaataatc cgaatattct atagtaactc ttggtggagg
    14281 tgatatttta cttttttcta agcttactat atcatcaact ctatcaacca caaatccaac
    14341 taccaagtcc tttatttcag ttactactat atacatgcaa tttgtagtca ttaattcatc
    14401 ttttttaagt ctcaaacgca tatctattat cggaatgatt tttcctctta gatttattat
    14461 gcctttagaa tatgtaggta gttctggaat aaatataatt tcttgatttc cagtaatttc
    14521 tattatcttt tcaagttcaa ccccaaaatc ttgctcatct actttaaact ttagatactt
    14581 aattccttca tcaattagtc cataatctaa atatgggtca tctggaaatc tcatttaata
    14641 cctccctcct aaaatacact ttctatgaaa tatatattat aatttcttct tacctttatt
    14701 tattgaatct acaagtaatt cacccgtctc agtgtaaaaa cgtattgttc taccataatt
    14761 catacctgtg tcctcagcta atattggaat atttaatcct aaaagagttt tttttactgc
    14821 ctcaacattc ctatgtccta tacttgcaat tactgaattg ctacttccta aaaacatctg
    14881 agcacctcca gcaatttttg cttttatata tattctattt gcacctatat tctccataga
    14941 tttaatgagc tcatttattc ctgtgtcagc aaatttacac ttattttcaa tgttaatagc
    15001 atctttacta tatggcaaca taacgtgtaa cattccacca attccagcta ctttatctat
    15061 caaacatact cctacacaag aaccaagtgc ataagtaact aaaacatttg gtctataggc
    15121 tatcttcata tctgcaatgt tcactactat ctctctattc atacttctaa tcccaacctc
    15181 acgagtattt ctctaagtga gctcatttct ggaatcatca aaatgtgact agtaaaataa
    15241 ttatcactca ttttaaattt attttctata aataagactt tgtcaccaac atctgaaaat
    15301 cttatcatag gcacatttag aacagctcca accatatcaa tacatatatc tggaattgaa
    15361 acacttatag tcatgtttaa catagatgca atagcattta catatgaact tgccataata
    15421 tttcctattt ccttaattgc tgaaatttca attgcctcaa tctcatctaa aaaagcttct
    15481 tctttattaa aaagagaact tagcataagt tgagttattg attcatccaa caaaaacata
    15541 aacattccat tgatatcacc tttcatatcg agtaaaatac atactacttt gttttctgga
    15601 ccaccaagca aatttgtcgc ttcattatat tcaagtatct tgaccatagg tattaatact
    15661 tctacattgc tatttatcat ggatgcaagt gcagtaattg cattcccaga gcctatatta
    15721 ccaatttctc taagagcatc tatatgaaca tcatcaagtt ctaaataatt tataatataa
    15781 acccctccct ttattattgc atttatttgc ttttcattgt atcagtgtat atttacactt
    15841 ttattttaaa acattattta caactttaac taatgtttct gttttgaagg gcttcactat
    15901 aaaatctaac gctccaagtt ttatggcttc tacaatcata ttctcttgtc ctattgcaga
    15961 gcacattaca accttagcac ttgaatccaa cttttttatt tctcttagca cctctaaacc
    16021 atcttttttt ggcattgtaa tatcaagcaa catcaaatca ggcttttcat atttaaatat
    16081 attacacgct tcttctccat catttgcttc tattaaatta ttatagccac ttttactcag
    16141 agcttcttta attatcattc tcataaaaat agcatcatct actatcaaaa ttttattttc
    16201 cataaaacta actcctccat atgtacaaca ataaacgctt ttatcttcag ttattgtcta
    16261 tactatctga ctatttttcg ctacacaata aatacatttc attgataaat aaaagtaatt
    16321 atttatgcca tattatactt aattctctta acataatttg tcaacaaaaa aacttatata
    16381 tctattgtaa tatatgtttg gcgttttgac gagttattaa aaatataatt gtataaaaca
    16441 taaaaagtta tcaaaagtaa ttaaattata ataaaacaac tttatacctt ttttatgttt
    16501 gtataaaaaa atttttcaga ttcattaaaa tcatttctgt agattcatat aggatttgac
    16561 tttctcaata tctaatagat agttgtttat ttacttaatt atatttctct atatgtatta
    16621 tcgattaaat tagatttagc tttatacatt attttttata tttttcaaat tgtataccca
    16681 taaatccaga agtattctaa tgtgtttata atactttttt tcatatttta aattaaataa
//
LOCUS       DAEMHW010000075        16630 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEMHW010000075 DAEMHW010000000
VERSION     DAEMHW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.075"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            111..473
                     /locus_tag="KPK67_002046"
     CDS             111..473
                     /locus_tag="KPK67_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBG2459014.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            909..1610
                     /locus_tag="KPK67_002047"
     CDS             909..1610
                     /locus_tag="KPK67_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBG2459015.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFSYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1881..2699
                     /gene="larE"
                     /locus_tag="KPK67_002048"
     CDS             1881..2699
                     /gene="larE"
                     /locus_tag="KPK67_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBG2459016.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2754..3902
                     /locus_tag="KPK67_002049"
     CDS             2754..3902
                     /locus_tag="KPK67_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBG2459017.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNEFKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            4038..5426
                     /locus_tag="KPK67_002050"
     CDS             4038..5426
                     /locus_tag="KPK67_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2459018.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            5455..6153
                     /locus_tag="KPK67_002051"
     CDS             5455..6153
                     /locus_tag="KPK67_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2459019.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            6156..7589
                     /locus_tag="KPK67_002052"
     CDS             6156..7589
                     /locus_tag="KPK67_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2459020.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            7611..8348
                     /gene="truA"
                     /locus_tag="KPK67_002053"
     CDS             7611..8348
                     /gene="truA"
                     /locus_tag="KPK67_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG2459021.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNIIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    8459..8666
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8919..10118
                     /locus_tag="KPK67_002054"
     CDS             8919..10118
                     /locus_tag="KPK67_002054"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBG2459022.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            10365..11411
                     /locus_tag="KPK67_002055"
     CDS             10365..11411
                     /locus_tag="KPK67_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBG2459023.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGEYLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            11519..12682
                     /locus_tag="KPK67_002056"
     CDS             11519..12682
                     /locus_tag="KPK67_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2459024.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMIQNYLVNPTWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSKEVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(12771..13151)
                     /locus_tag="KPK67_002057"
     CDS             complement(12771..13151)
                     /locus_tag="KPK67_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBG2459025.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(13154..13813)
                     /locus_tag="KPK67_002058"
     CDS             complement(13154..13813)
                     /locus_tag="KPK67_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBG2459026.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(13919..14893)
                     /locus_tag="KPK67_002059"
     CDS             complement(13919..14893)
                     /locus_tag="KPK67_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG2459027.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(14933..15280)
                     /locus_tag="KPK67_002060"
     CDS             complement(14933..15280)
                     /locus_tag="KPK67_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459028.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(15492..16448)
                     /locus_tag="KPK67_002061"
     CDS             complement(15492..16448)
                     /locus_tag="KPK67_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBG2459029.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNLFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
BASE COUNT         6549 a         1745 c         2680 g         5656 t
ORIGIN      
        1 aaatatgata attttttatg ttgaaagctt ataatgatta tgatataaat tggttatagt
       61 atacaattta tgggtataac aataaaaaat tagaaaggaa tttttataaa atgattaaat
      121 tatatggata tacaaaatgc tctactgtta aaaaagctaa aaattggctt aaggaaaata
      181 atttagaatt tgaagatata gatatggttc aaaaccctcc tagtaaagag gagttaaaat
      241 caatttataa gactagtgga tatgatataa agaaattttt taatacaagt ggtatgaaat
      301 atagagaatt gggtcttaaa gatattgtaa aaactgaatc tgatgataaa cttttagaaa
      361 tattggtaag tgatggtatg cttattaaaa gaccattact tttggatggt aaaaatgtat
      421 tattgggctt taaagaagat gtatggaaaa gtactttgct aaaagaagat taggaagaga
      481 taggtgtgtt gattaaaatt attaattaat acacctataa atttatgtat taaatctaat
      541 ttttataaat attaattagc aaattcaaat caaaaaaatt catatcaaca aacatattac
      601 ttcttattac aaattatatc atatgtttta tattaaaata ttataatatt acaacgaatt
      661 gttacaaaat tacttattat taaccatgaa catttgtttt gtgctatcat atttatataa
      721 gggaattgtc ataaaaaata tcttgttcat acagttttat atagaaaaaa gaaatgaatc
      781 ttttctttat ataagtatat tttgtaaatt gcctctatat ttatagttta aataatagtg
      841 gtattaaaaa tctgacaaca aaaataaaac tttcaaaaaa ttaaatctaa aaagaatagg
      901 agataaatat gagtattgaa gatttatatg attttgaatg tgttcctttg aacaatgaga
      961 ttgatattga tgttttattt gaaaagatga tagaagatgg aaaagaaaaa gaatatacac
     1021 caattatagt cattgatgaa aaagtagggc tattaagaaa aaatatagat atgattacta
     1081 aagagtatgg ttctcttgaa aactatagag agtattgttt aacaaaatat aatgaaattg
     1141 atgtaaatca attttttgat tttaggaaga gtgagatttc agaattatta aaggagctaa
     1201 ttgatttaga agatgatttt tatgattata aacattttag accaattatg aatatagata
     1261 tatttcatga atataacaaa gtttacatag ctaaaattcc aacaattaaa ccatatgaag
     1321 tattttcata catacctatt ggcggattta atgattgtcc tagagatgaa gaacatatag
     1381 ctattgcaaa atattggtat gaaaagcatg gaatctttcc aatcgcaatt ggatgtgata
     1441 cagttcaata ttctgtaaaa aatttaacta aagatggtaa aaaatttgat gacttatgta
     1501 ttgaattagt attttattgt gaggatatta ttatacaagg gtatgaaacc ttaaaggctc
     1561 ttaaggatgt attaaaacgc tctacaatag tgcttttttg gtgggattga tattctatca
     1621 tttagatagt attaaattgt tagtcaccaa gaatccactt gctttagtta tggggagtgt
     1681 gtcaaactag gtttttatac acagtgtagc atagtaatat gtttaaatta caaaggttaa
     1741 aaaaatactt taatattttt aatattataa ttaatagatt taaaggtaat tataatttat
     1801 aaaataaata ctttatatgt tataatcatt aagaatgtaa aaaaataata tatagtttta
     1861 tagttcatat ttttccagat atggaggtaa attttgtgaa ggaaaaagaa aaattagata
     1921 aattaaaaaa aatgttactt gaattaggca gtgttgtagt tgcttattct ggtggagtag
     1981 atagtaattt tttgcttaag gtcgcaaaag atacattagg tgaaaatgtt atagctgtga
     2041 ctattcatgc aatgatgcat tcaagtaggg aaattgaaga ggctaaacaa tatactcaaa
     2101 attttggtgt taaacatatc atattaaata tagaaaactt tgatttaaaa gaatttaaag
     2161 aaaatggtgt tgatagatgt tatcattgta aaaaatatat attctctaaa ataaaagagg
     2221 tagcaaaaga acataatata aagtacatag tagatggaac aaatatagat gatttaggag
     2281 actatagacc tgggcttaag gcattgagtg aattaggagt aattagtcca ttaaaagata
     2341 gtagtttaaa aaaagaagaa attaggtcgc tttcaaaaac attaggatta aatactttta
     2401 ataaaccatc atttgcatgt cttgcgagta ggatacctta tggagttgaa ataacagatg
     2461 aaaatttaag aatagtagaa aagagtgaag aatatctatc caatttaggt ttttctcaat
     2521 ttagagtgag aatgcatgga gatatagcta gaatagaagt tggacaggaa gagctaggta
     2581 aattttttga aaataacaat tttaataaag ttgatactaa attaaaaata tttggcttta
     2641 aatatgttac actagatatg tcaggatata aaatgggaag tatgaattta aatgtgtaga
     2701 ttattttaaa aataaataag ttttatagtt aatataagga aacaggaggt tttatgaaaa
     2761 attatacgtt aatatcgcca tgtttttttg gaatggaaaa aatgctggct agagagatta
     2821 ctaatttagg atatgaaata ataaaaacag aagatggaag aataacatat aaaactgatg
     2881 agtttggaat agcaaagtca aacatgtggt tgagatgtgc tgaaagagta catcttaaaa
     2941 ttgctgaatt tgaggcaaaa agctttgatg aattatttga aaatacaaaa agaataaact
     3001 ggtctagata tataccttat ggagctcaat ttcctatatc aaaagcttcc tctataaaat
     3061 caaaattata cagtacacca gatgtacaag ccatagtaaa aaaggctata gtagaaagct
     3121 taaagaaaag ttatttggag gatggtttgc ttaaagaaga taaggagaaa taccctattt
     3181 ttgtatttat acacaaagat aaagtaacca tttctataga tacaacagga gatgctttgc
     3241 ataagagagg atatagagaa aaggcaaata aagctccaat aagagagact ttagctgctg
     3301 ggcttattta tttaactccc tggaaagcag gtagagtttt agtagaccct atgtgtggtt
     3361 ctggtactat attgattgaa gctgctatga ttggtataaa tatggcacct ggcttaaata
     3421 gagagtttat atcagaaaag tggagaactc ttgataagaa aatatggtgg gatgtaagaa
     3481 aagatgcttt taataagata gataatgaat ttaaatttaa gatttatgga tatgatatag
     3541 atgaagaatc tatagatata gctagagaaa atgctgaaat agcaggtgta gatgaatata
     3601 tagagtttaa tgtaggagat gcaactcaat ttaaaagtga agatgagttt ggatttataa
     3661 tcactaatcc tccttacgga gaaagattag aagataaaga cagtgttaaa cagttgtata
     3721 aggagcttgg ttatgcattt agaaaattaa aaaattggtc gtattatttg ataacttctt
     3781 atgaagactt tgaatatgag tttgggcaaa aagcagataa aaaaagaaaa ttgtataatg
     3841 gtatgttaaa aactaacttt ttccaatatc cgggtcctaa gcctcctaga aataataaat
     3901 aagttttaca tgatttattt gtaaaatata aaattttatt aaaataagta aaacattatg
     3961 atgccttgaa acgtctaatt attgtgagga gatttttgtg aacaaattta aagatttata
     4021 ggattaggag gagttaaatg aaagtgttaa agcttattac tgtagtagcc ctgatgatgt
     4081 tatttgttac tggatgtaat ataaaaactg aatctccaga acaattagcg aagatacctg
     4141 actatgatga tacaaaaaaa gtattgtatg atggtattga ccagttacta aaaccagatt
     4201 catctatgat attgccttct aatacaaaag aagtaggaaa aataaataaa gtagatttaa
     4261 acagcgatgg aattgatgaa ttagtagtat ttgaacaaaa agaagattta agtaataatg
     4321 tgagtcaggt agggtttgta accttgagtt acaatggaga aaaatacgta cttggagacc
     4381 attttttaga aaatggcgaa tcaatagaat atgctaattt ttatgatttg gactcagatg
     4441 gatataagga agttatctta ttggttaaat ccaaagacaa aacgaatatg catatatata
     4501 aagtagtaga taatgaaata acaaaaatat atgacttgga tgcatcatgg cttcaaaata
     4561 aagaagattt taatgacatg aaagtaaaaa ttggatatat agatggagat gacaagttag
     4621 acatattgat gcttcatttg aataataaga ctaatgaaat gtttgcaagt gttgcaaatt
     4681 ttgatgggaa aatgaaaatc aaagattctg ttaagtttga aaatgtaaaa aacttaagtg
     4741 aattatatat aacattaggt aatgttgctt catctgttgg aaattcttct gcagctataa
     4801 aaggtattgt acttgatatt cctatattga aagataataa ctatattacg cagatacttt
     4861 atatgaaaga tgggaaaatt aacaaagctt ttagtgatta tgataaaact attactaaat
     4921 cttattatat acctgtagat gatattgaca aagataagat tatagaaata cctatagttg
     4981 caggcagtac aggtaataat aaaaatactt atagttctaa atcatctgca actataagtt
     5041 ggtatagatg gaatggaaaa gaaggtgctg actcaagttt aatatttaca agtcagatat
     5101 attataatta taaatataat tttaaattat ttattccaaa taatttattt gataaaatac
     5161 ttgttgaaca ggaatttgtt ggagaaaaag cagtatttaa attttactat tttgactatt
     5221 cagaccgtaa gaatttattt aatatagtag tagagagtaa aaataaactt gaagatagaa
     5281 aaaatacagg tactcaaaat tctattgtac ttcaagagag cgatgaatat actttcttac
     5341 tagttgtaaa caatgcaaat gaaatggata agctagatat gactatagac gcattaaaag
     5401 aatatttctc attaatatat ggttaatatt atactcaatt ggaggtttta ttttatgaag
     5461 gaaaagatac ttatactaga agatgaaatt ggtattagaa gttttgttag tataaactta
     5521 aaaagggaag gctatgaaat agttgaggca ggtactggaa gagaagctat agaaaaaatg
     5581 acaacagaaa aggatattac aatagctctt ttagatgtaa tgcttccaga tataagtggt
     5641 atagaagtat gtaaatttat aagggagaat tttgaccaag taggtataat aatgcttact
     5701 gctaaagctc aagaagatga taaaatagaa ggatttatct caggagcaga tgattacatc
     5761 ataaaaccat ttagtataaa agagctttta gtcagagttt ctgctcttct tagaagagta
     5821 gcaaaggatg attcaagtgt taagtcgagt gagatagttt ctccaccatt tatacttgat
     5881 atagacaaaa gaaaattatt taaaaatgga aaggaaattg agcttacacc aactgaattt
     5941 tcaatagtga aatatctaat atcaaatgct aagcagtcgt taagtagaga ccagatatta
     6001 gacgaagtat ggggaactaa ctatttatat gattttaaaa tagtagatgt aaatataaga
     6061 aggataagaa ataaaataga agatgaccca tcaaaaccaa aatatataca aactatttgg
     6121 ggttatggat actgttttag aaaggaagaa taatagtgtt acattttgaa ggtctgagaa
     6181 aaaaagtaat aaaaaactat tttataataa ttattataat ggttactctg tttgaaggat
     6241 tgttcatgtt ttacatacaa aactactatt atgattctgt caaacagcta cttgaatctg
     6301 aaataaaata tgctgatgaa tataatgcta ttacaatgga aactacaagt tttgagaaaa
     6361 aagtaaaaaa tatatttgat aaacaaccct taactaaaaa ttcagaattt ggaatttcaa
     6421 taatagataa agataaaaat attatattag accaatatgg ttttaaaagt aaagaaaaag
     6481 caaactatga agatgttaac aatgcattaa aagatataaa aactaaaaat ttaacacctt
     6541 atacttatag aataccagat acaggggagc atgttatgag tatctcctta ccacttaagg
     6601 taaataatat aatagaaggt gttgttagat atacagtatc attagatgct attgataatg
     6661 caatattaaa gcaagctaca tggcttatat tagcaggaat atttatactt attatagcta
     6721 tactaataag tcttaaattt gctgagactc ttataaaacc attacgagag ctaaagaaat
     6781 ttgcaaatga attggctgta ggaaattata atataaaatt agaaaagatg aagattgtag
     6841 atgatgaaat tggtgattta gcacagacat ttgaacacat ggcacatgag attgataaaa
     6901 gtgaaaaatt aaaagaggaa tttatatcat ctgtatctca tgaactaagg actcctctta
     6961 catctataaa ggggtggagt gaaaccttag gttatgaaag tataacaaga gaagaactag
     7021 acttaggttt aggcattata caagatgaaa cagaaagact tataaaacta gttgaagaat
     7081 tattagactt ttcaaggctt tcttcagaca ggataaaact tcatgttgat atagttgatg
     7141 ttgaaggttt aattgtaggt gttgtaaatc aacttaaagt taaagcagct gaaaaagata
     7201 tatcactttt atttgaattt gaaaatgaat ttatagaaaa cattcaagga gataaaaata
     7261 gattaagaca ggttctcata aatttaatac aaaattcatt taaatttacg agtcaaggtg
     7321 gttacataaa agttgtagct tctcaagatg aagagataac aaccatatca gtagaggata
     7381 atggttcagg tatagaaaag caaaatttaa ataaagtgtt agataaattt ttccaagaag
     7441 attataataa ggctggtagt ggattaggat tagcaattag taatgaaatt gtaaaacttc
     7501 atggtggaag aatgaaaata gaaagtgaaa agaatgttgg aacaaaaata acttttaata
     7561 taaaaaataa atttgctaaa caagcataaa ttcttagaaa gaggattaag atgagaaaca
     7621 taaaaatgat agtagcatac gatggttcaa gatataaagg atatcaaaaa ttaggtgaca
     7681 ataatatgac tattcaagaa aaacttgaaa atgttttaag taaaatgact aatgaaacag
     7741 tggagataat aggttctgga agaactgata tgggagctca tgcaagagga caagtggtaa
     7801 attttagaac taattgtatg gattctttgg ataaaattca aaaatattta tatgaatatt
     7861 taccagaaga tatagttgta aaaactgttg aagaagttga tgaaagattt cattcaagat
     7921 acaatgtaaa gtcaaagact tatatgtata aaattgacaa taataagtat cataatcctt
     7981 ttataagaaa atatgctact catgtaagta aaaaattaga tttagataga atgagaaaag
     8041 caagtgagta tttagtagga gaacatgatt ttaccagttt tgcttcttct aaatctaaga
     8101 aaaaatcaaa tgtaagagaa atttattcta taaatataaa agaagatgat aatattatag
     8161 aaatatatgt agaaggaaat ggatttttgt ataacatggt aaggataata gtaggtgctt
     8221 tgatagatgt tggtctaaaa agaagagctc cacaagatat taagtatatg cttgaatcaa
     8281 aagatagatg tcaatcatca gatactgcac cagctaaagg actatgtctt tggaaagtaa
     8341 catattaatc aattatataa aactctcttt aaaataaaaa taaaataaat tatatagttg
     8401 acataaatgg aaaaataaac tattatatat tttataagag agatataaaa ctttatattt
     8461 aaatcctgtg agaaagagag taacttctat gtgttttata gagagctgag gatggtggaa
     8521 gcttggcaaa taagtattag tgaaaagaac tttggaggat actaattgaa tttagtatgt
     8581 tagtaggtaa tctcgcctta agagaaagag tggataagtt tatcttatca attagagtgg
     8641 taacgcggat atatttcgtc tcttggtttt tccaagaggc gttttttaat atatttaatt
     8701 attagtgttt atctggtatg ttgaacattt actaattgat tagctaagtt tatcaataag
     8761 agtggtaccg cggatatttc gcctcttagt attttaaaat actaaggggt ttttttagta
     8821 ttaaaatgat tttatgtgag tctaaattct cagagtcatt caatattaag aatttctata
     8881 aaataaaaat tataatttaa gattgggagg aattaaaaat gaaggaaaaa gtagtattag
     8941 catattcagg aggacttgac acatcaatca taataccttg gttaaaggaa aattatgaag
     9001 atatagatgt tatagctgtt tgtggaaatg taggtcagga agataaaatg gaagatgtat
     9061 atgaaaaagc tttacaaagt ggagcatcaa aggcatatgt ggatgatata agtgaagaat
     9121 ttgtaactga aactatattt aaagccgtaa aagcagaggc taaatatgaa ggaaaatact
     9181 tattaggaac ttctttagca agacctataa tagctaaaaa attagttgaa gttgctcata
     9241 aagaaggtgc aaaatatata tgccatggat gtacaggaaa aggtaatgac caagttagat
     9301 ttgaagctac aattgcagct cttgacccga ctataaaagt tattgcacct tggagaattt
     9361 gggatataaa atcaagagaa gatgctatag actatgctga aaaacataat ataaaggtaa
     9421 cagcaactaa agctaaaata tattcagtag atgctaacct ttggcatgtt tcaacagaag
     9481 gtggagatat agagcaccta gaaaatgaac ataaaaaaga tgtttataaa caatgtgtag
     9541 acccagaaga tgcatgtgat gtagctgaat atgtagaagt ttactttgaa aaaggtgtac
     9601 ctaaaaaggt aaatggcgaa gaattatctc cagttgcact gattcataag ttaaatgagt
     9661 tgggttgtaa acatggaatt ggtgttatag atatagttga gaatagactt gttggtatga
     9721 aatcaagagg tatatatgag actccaggag gaacaatcct ttatgaagca cataatattt
     9781 tagaaagtgc tactttagac aaagatacat tacatttcaa acaaatggta tcttataaat
     9841 acggagaact tatttacaat ggactatggt attgtaaatt aagagaatct atagatgcat
     9901 ttatggaaca aactcaagat aatgttacag gaacagtaaa agttaaactt tacaaaggaa
     9961 atataaaacc tgctggaata tttactgaaa atgctcttta tgatgaagga atatcttctt
    10021 ttggaaatag tgaactttat gaccacaaag atgcagaagg atttattaat ttattcactt
    10081 taccattaaa aatcagagct atgaaagctg gaaaataata aaataaatta tttaagagtg
    10141 tataaaaact atttttaggt ttttatatgc tcttttttta tttgaattta ttaaattata
    10201 cagtaaatag tataaaaaaa tatattatag ttccaaatgg cttaataatg tgaaaatata
    10261 agacctaaaa tataaatttt ataaatacaa tagttattga ctctctcatt gtaagagggt
    10321 ttatactgaa tctaatgaaa atttatagtg aggagataga ttatatgaga aaaataattg
    10381 aaattaaaca tcattcacat gattatgagt gtatgtggaa tggtatagaa gatttatata
    10441 taaataaaac cggtgaatac cttccaaacg attttttctt tttactgtca ggttttggtt
    10501 cattttgtta tatgaaaaca aataaagctg atttgaaaag aatggtagct ttgggagatg
    10561 gaaggacaaa acagatgtat aaatttttag caccaattgt tggatttgaa tacaaacatc
    10621 atgagtttaa aaagtatgag caagcattaa agaaagcaaa atctgaaatt gatgatggat
    10681 atccagttat tttaggagct ttagatatgt attatctacc atattataag aagctatatc
    10741 ataaagaaca tattcctttt cattatatat taatggtagg atatgatgat gaaggaatat
    10801 atctgtatga ttgtggtaga acagaattat tacatctttc ttatgataaa ttacgtgaaa
    10861 gcatgaattg caattcccct ggattaagca aagaaaatac tatttgcaca ataagaatga
    10921 atacttcaaa gaataaatat aacattgcta gagaagctat tgctataaag cgtgatatgt
    10981 ttttaaatcc acctaaagga tttttgggat ataaaggttt tgaaaagttt attaaagagt
    11041 tgccacagtg gaacactgaa ttggataaag atgattatga taagattttg accaatatgg
    11101 ttatgttttt tggaacagta ccaacaattc caaatgcaat caaaggaatt aaagaaccag
    11161 atactgttca atttaaaggt ggatttgata aaatgagtag aatgctcaac agtattgggg
    11221 aggaatacaa aaacgactct tttatacaag tagctgatat ttttgataaa actgctatta
    11281 taattgagaa aatatccaat attataatag attatttaac tcaaacatgt gatgatacag
    11341 aacaacttcc gttattgttc tctgaggtct tagagcatat gaaaacagga tatttaatct
    11401 tggatgtata aaaaatatat tgttgaccct aacattacgt aatagtgtaa tatgaaaata
    11461 tagcttgaat atattagtag gtattgatgg ctgtaaaatt aaaatttgag gtgtggatat
    11521 gagaacagtg aaacaagttt ctgatttaac tggaataagt gtgagggcat tacattatta
    11581 tgatgaaata ggtcttttaa aaccaaataa aattacagat gcaggatata gactttatga
    11641 tgatgaatct attaaaactc ttcaacaaat tttatttttt aaggagattg atattccttt
    11701 aagagaggtt aaggaaataa tgtcaagcca atattttgat aaggttgaag ctctaaaaaa
    11761 tcaaaaaaaa ttacttattt taaaacgaaa gagactggat gaattgatag agcttataaa
    11821 tcaaactcta agaggagaag gaaatattga ttttaaagag tttgatatga gcgagtattt
    11881 taatgtttta gaagaattta aaagagaaca taggaataag gtaattaaaa tttatggtag
    11941 tgtagaaaaa tacaatgaat atatagaaag agttaagtct aatgaagaga aaattgctaa
    12001 aatggctata aaacaatatg gaactattga gaagtttgcc caagctataa aaacaaattt
    12061 tagcagtgat atattgaatt taggagaaaa atttgataga tataaaaatg attgtttaaa
    12121 agaaaaacat ccaaaattaa aagaattata tagaaagctt gtagaagatt taagtaggaa
    12181 tccttcttca acagaccttc aagaaatagc aaaagagata acagatatat ctaaaaaaga
    12241 ttatgaaatt ttctctatgg atactggtga tgacaactgg tattatatga ttcaaaatta
    12301 tctagtaaat cctacgtgga tagaagaagt agataaaaag tatggaagtg gagcaggaaa
    12361 gtttattgga caagtactaa agacttatct aagagataga aaaccaaaga taaatacatt
    12421 gtatgaaaaa cttgtagaag atttgagtag agattgttct tcaaaggaag ttcaaagcat
    12481 tgttgaagaa atagacaatg aaatgaaaag aagtaatgaa ttttataaaa tagacaatgg
    12541 cgaaagatac tttgattata tgtcagagct ttatttacaa gattctaact atataaaagt
    12601 aactgataag aattatggtg atggtgcatc taagtttatt ggagaagctt ttaagattta
    12661 ttttgataat aataattgtt gaaaatttgg aagtaaaaaa tatgtaagtt ttataaaagc
    12721 acaaaaataa ggatgtagat tagctccatc tatatcctta tttaaagtta ttaaaatata
    12781 tagtttacat caaacttaac actttcaata atatttccca aatatgtttt tatactttca
    12841 acttcttcta aagattttaa tttattttca gtataaattg ctttgtccag aatattaaac
    12901 cttaatccta tttcatcaaa atactcatga tttaaaactt tgtaccaatg gtttttttct
    12961 ttattccaac tcgtagatat tgaatcagct tctttttgag ctttttccca atcttcattt
    13021 tctatagatt tttctaaaac agatatattg tctttatatc cttctgtaaa atcatatagt
    13081 ttattgttaa cgtaaaatcc aaataataca aataagatag tccatagtat aacaaatgtt
    13141 gtagatttca atattaatca ccctttttat tttgaataaa tattttgtca ttttcatcta
    13201 aaacacaaat aagaacatct ttaaaagttt ctatgtgatt agattttaaa atacccatta
    13261 accagttagt atctttttga gctatgtcta gatttttatt tattattcta ccatccagta
    13321 taagtgggat aggtaaatga ttaaaagctc ttggaggagt actattgtag cttgatgctg
    13381 gtacaacact tagattacca tcagtctcca aaatagcata ttgtacatct tttaggttaa
    13441 agtatccttg aatcctaagt tgttctaata attcatctat actaattctt tcttttttaa
    13501 gctctttata tacaattttt cctttatcta ttaatacaga aggtttacct gataaaaatg
    13561 aacttaattt tctacttttt aatgataaga atgaaattag agtctgcatg attacaagtc
    13621 cagatattgc tgcaacacca tttatgattg gtatattatt attttccatt gggacagatg
    13681 caacttctgc aattaatagt gttataacta aatcaaaaca gttcatttca gctatttcac
    13741 cttttcccat gactctaagt gcaattagca ctgttatgta taatataata cttcttatta
    13801 atactactgt catatctatc aattactcct ttaaaaaata tttacactta gttagtatat
    13861 cctatataaa aaatttaatt caaagtataa aaaaccttaa ataattggat tcaactattt
    13921 aaggtttttt tgataatttt aatattaaat tttttatgga ttttctgtta cttttattta
    13981 ttttaataac agattcaaca aaactctttt tatctcctaa ttttaagata aaatcaggat
    14041 attcttcaaa ttcattgagt tttattaatt tgaaagaaga agatagattt ttaaagttaa
    14101 taattttaga gttattcact aatacacttc tttttttaaa ataaatttct tttttactta
    14161 tatttagtat agttaatatt tgtttaagat atattattat atcttctgga gttctatcaa
    14221 atttgagttc aaaattttca tcttcaaagt agggaatata atataaaggt gttttgagag
    14281 caggtaattt attatataat tcttcaagaa tatcgatatt tttaggggag ttaaagaaaa
    14341 agcatttaca tacattcatt aaatccacag catctgaaag tgagttatgt ttaatatctc
    14401 cttctacaga aaaaattttt ttcatattca gtaaagatat ttgcttagat aagattctat
    14461 tattgtatgt aatatgttga gatataaaag gttgaatgtc aatcatcata ttaaataact
    14521 ctaagcattt acctgtatat ttatacactc taaatgaagt tagaaggcca gttatatcaa
    14581 aatcacccca attatatatt gtaacatcat caaatttaca aaaccattta taaaaatcat
    14641 ccataacata taaaagatta ggagcattat tgatatctag ttggttcaat ttggttaaat
    14701 ttttacattt atttgaaagt attttattgt attttggctt aactaaagag taataattat
    14761 ctattgtatt aaattcgtta tcaactaaaa ctccaccaat agaaattaat tccgcaccat
    14821 tatattcttt tggtttattt ttattatcat ctagtatatt aaattcaaag tctataaata
    14881 acttgtattt cataaatcta aagcacatcc tcaggtaaat tatttattga gcctaagcat
    14941 cataagaatc aatagatata atgactctaa acatcaaagg ttcattacaa cttttattta
    15001 tttcataatt taattcaaat aaatctttta agatataatt tataagtatt ctttcccaac
    15061 ccatttttaa attaacttta gagtctatta atatattttc tatcttttta aatatttcta
    15121 tatttgctgg tattatactg agctcaatat tacttttaaa cgggttagac tgtaaatctg
    15181 ttataagtat attttttaat tcctctgtaa gttcagaaat taaactatca cctatgtatt
    15241 tttttatgat ttcctctcta atttctttta aatttagcaa tattagtacc tgctacttac
    15301 atagtagctt ttccttttaa tactattatt catttttgaa tcttatttag atatatttta
    15361 agattaaaag tctttagcta tataattttt tacccatttt taacttatat taacatatta
    15421 cagcttatat ctccaccctg cttacattga ggatggagta tattgcctat tttgataaaa
    15481 atgataggtt gttatttgcc tattcctttt tctgataatt tttctaacat atctttggtc
    15541 attttatcta aatcataact aaacttaaag ttccattctt ttactgcact actactgtct
    15601 agagagttag gccaagaatc agctatactt tgtcttacag ggtctacatc atattctatc
    15661 acaaaatcag gaatatattt ttttatagaa tcagctattt cttcaggaga aaagctcata
    15721 gcagtaatat taaaagcatt tctatggatg agtttatccg ctggagcttc gattaaatcc
    15781 actattgatt gaagtgcatc aggcatatac atcatatcca tttttgtacc ttctgctatg
    15841 taagatttat atctcttatt ttttaatgcc tcataatata tatcaactgc ataatctgta
    15901 gttcctccac caggaggcgt aacataagaa ataagacctg gaaatctaac acctctagtg
    15961 tctactccaa attttgagtg ataataatca catagtaatt ctccagaaac tttagtcaca
    16021 ccatacatag tattaggtct ttgtaatgta tcttgaggag tcatattttt tggagaattt
    16081 tcaccaaaag cagcaattga actaggtgta aaaaatttac aatccaattc tttggctatt
    16141 tcaagaccat ttattaatcc attcatattt aagttccaag cttctagagg tttactttca
    16201 gcaactgcag aaagtaaaga tgctagatga attatagtat ctacatcata tttttttgca
    16261 atttcaaaaa aaagattctt atctgtaaca tctaatatct caaatatacc agattcacat
    16321 actggattac cttcttttac tctccttgaa ctagcgatta cattttgttc tccatactca
    16381 tttctaagct ttattgttaa ttctgaacct atttgtccta atgcacctgt tataagtatt
    16441 tttttcaatt ttacaacctc ctaagggttt aatttatgta gtactactta tttttatttt
    16501 agcaaatctt ttgtttaaat acaaagaatt aaagataata atatacgact taattgaatg
    16561 aagtgcagta agtgcctaaa aatttaaagc taatatataa tggtctattg tataacaata
    16621 aaagagtaga
//
LOCUS       DAEMHW010000076        16598 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEMHW010000076 DAEMHW010000000
VERSION     DAEMHW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.076"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1045
                     /locus_tag="KPK67_002062"
     CDS             <1..1045
                     /locus_tag="KPK67_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2459030.1"
                     /translation="EQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGF
                     IYWYSSKKFNIKVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNT
                     SITEEIQAFLFSIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKS
                     RLSQTISFRHDFNNHLAIVNGLLKKDQILKAQDYLNKLERVTSDLSFSCNTQNIVIDA
                     LFNNKLSIAKQLGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYL
                     KLSTKLEGGFFMIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQI
                     NVLLVISLHCASI"
     gene            1084..1569
                     /locus_tag="KPK67_002063"
     CDS             1084..1569
                     /locus_tag="KPK67_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459031.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKSLQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKDLKVKFNRP
                     I"
     gene            complement(1832..2068)
                     /locus_tag="KPK67_002064"
     CDS             complement(1832..2068)
                     /locus_tag="KPK67_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459032.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSGYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTDKIQTAS"
     gene            complement(2223..2447)
                     /locus_tag="KPK67_002065"
     CDS             complement(2223..2447)
                     /locus_tag="KPK67_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2459033.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(2544..3797)
                     /locus_tag="KPK67_002066"
     CDS             complement(2544..3797)
                     /locus_tag="KPK67_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBG2459034.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            4057..5046
                     /locus_tag="KPK67_002067"
     CDS             4057..5046
                     /locus_tag="KPK67_002067"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBG2459035.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRVTFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            5118..5858
                     /locus_tag="KPK67_002068"
     CDS             5118..5858
                     /locus_tag="KPK67_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBG2459036.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEERKKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            6058..7209
                     /locus_tag="KPK67_002069"
     CDS             6058..7209
                     /locus_tag="KPK67_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG2459037.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVIITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            7234..7989
                     /locus_tag="KPK67_002070"
     CDS             7234..7989
                     /locus_tag="KPK67_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBG2459038.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            8181..10568
                     /locus_tag="KPK67_002071"
     CDS             8181..10568
                     /locus_tag="KPK67_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBG2459039.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMDGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGISRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(10799..11032)
                     /locus_tag="KPK67_002072"
     CDS             complement(10799..11032)
                     /locus_tag="KPK67_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBG2459040.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            11321..11911
                     /locus_tag="KPK67_002073"
     CDS             11321..11911
                     /locus_tag="KPK67_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2459041.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            12025..12870
                     /locus_tag="KPK67_002074"
     CDS             12025..12870
                     /locus_tag="KPK67_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459042.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            12867..13550
                     /locus_tag="KPK67_002075"
     CDS             12867..13550
                     /locus_tag="KPK67_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2459043.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            13547..14263
                     /locus_tag="KPK67_002076"
     CDS             13547..14263
                     /locus_tag="KPK67_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2459044.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISFLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYIYMFISI
                     VISLIWILLLFKKFSKKIIK"
     gene            14360..14623
                     /locus_tag="KPK67_002077"
     CDS             14360..14623
                     /locus_tag="KPK67_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2459045.1"
                     /translation="MKNKIKILREKLGFTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEAIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            14595..15050
                     /gene="def"
                     /locus_tag="KPK67_002078"
     CDS             14595..15050
                     /gene="def"
                     /locus_tag="KPK67_002078"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG2459046.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            15102..15563
                     /locus_tag="KPK67_002079"
     CDS             15102..15563
                     /locus_tag="KPK67_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBG2459047.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            15613..16515
                     /locus_tag="KPK67_002080"
     CDS             15613..16515
                     /locus_tag="KPK67_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2459048.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
BASE COUNT         6625 a         1764 c         2677 g         5532 t
ORIGIN      
        1 tgaacaatta gtatatggat ttacagctcc attaaattac ttgatattta ctagtgatat
       61 aaatacagat atttataaat ttgatataaa tatgggacta tcttctttaa ttactattat
      121 aatagctgga tttatatatt ggtatagttc taaaaagttt aatattaagg tcatgaactt
      181 tgataaatat atagcaattt tgatgattcc attgttgctt ataatattat ttatgcaatc
      241 atttgaatat agttcaaata taaatataga tacaagtctt gggattgtaa agctgctatt
      301 aaatacttct ataactgaag aaattcaagc ttttcttttt tctataattg gaactatatg
      361 tgtatttttt tctctattta cctttaagaa actaattcaa gctctcgaag atgacaagga
      421 gagggcaatt atgaatcaac agattcatgc tcaaaaaaac tatattgaag aagcgaaatc
      481 acgattatca caaactatat cctttagaca tgattttaat aatcatttag ctattgtcaa
      541 tggattgttg aaaaaagacc aaattttaaa agcacaagat tatttaaata agcttgaaag
      601 agtaacaagt gacctatcat tttcttgtaa tacacagaat attgttattg atgctctttt
      661 caacaataaa ttaagtatag caaaacaatt aggcatagag gtagattgtg aagtaattat
      721 accccaaagc actaacatga acgattttga cctatgtata gtgtttgcta atgctattga
      781 caatgcaatt aaagcttgta gagttgtaga taaacaaaat aggtatttaa agctttccac
      841 taagttagag ggtggatttt ttatgataga aataaaaaat agctataatc caataaataa
      901 ttatacaagt ggctcaggta taggtctttt aaatataaaa gaaattgcta aaaaatatca
      961 aggggctata agtatagata agacatcaga ctattttcaa ataaatgtat tgctagttat
     1021 ttcattacat tgcgctagta tttgagaaca atttcattga aaatagtatt tgtaagctat
     1081 attatgtact tgaatagaga aagtttgata tatagaaatg aggaggttac taaaatgaaa
     1141 gttggaaata taaatggtca agcaccaatt acaaatggtt tagatagttg tcaaagtgag
     1201 gtacaaggct tagaacaaaa gaaaaaatca ttacaggatg aaatagagaa agtaagaagt
     1261 gataaaagta ttgatggtga aacagccaaa ttaaaaatag aagctttaaa aaaagaaatg
     1321 gaagagattg aaaataaaat tcaagagtta aaaggaaata aagcaaaaaa aacaaataaa
     1381 gaagaaaata atgtttcaga agtaaataat aatctattag agagaaaaaa agaaccttct
     1441 aataatgaaa atgtaaaaca attatatgat gtttttgaaa gaagcaatgt agacgaacaa
     1501 tgggataata cttatcgtgt tctccaagat gataaagact taaaagtaaa atttaataga
     1561 cctatatagt ttatttataa aaatagttaa tattttaatt atcaccactt tttagaagaa
     1621 tacacacttt attttaaaga cccacatatt tatttacagt tagcacattt atatgtggtt
     1681 ttataactat tatttacaag tctatattga taacttttac atagtatata atgtttaaat
     1741 acttattcaa atttatctat acataaacaa aaaaagagat tttaaaatct ctttttagat
     1801 tgttgactta tggctttttg atttaataaa atcaagaagc cgtttgaatt ttatctgttg
     1861 gctgataccc ttctgatatt gaaacaccat ttttgtttcc aaaatcgttt aaaattaaga
     1921 acttattatg aatgactatt tcattatgaa caataagcaa ataacctgaa tcttcaatca
     1981 atgttttaac agtagcatta aatgcttgct tatacagttc tatcgtttgt ttgaactgcc
     2041 cattaaagta aatatgagtc aataacataa ctatatctac acaaattaat ataaattttt
     2101 tatactgtac attatctaac tgttgattat ctgtattaca acatgattga atttaaaaag
     2161 ccatcaacac atagaatgtt gatggcctaa aaatagattt aaaatctctt ttttattata
     2221 tattaattat tttttaaatt tatatttttt gcatcagatt ttctaatagc taagtcaaac
     2281 cctctaaatc taatcactat agggtcatta agaggagcta tctttttcac ttcaacttca
     2341 gtgccatcta ttagtcctaa ggctagaagt ctcttcataa gtttttcatt tccagctata
     2401 ttgtctataa ttcccttttg tccaagtttt aagttgtaaa cagtcattat taacacctcc
     2461 taatgaaaat cattatcaaa ctttatattt ataatatata acaaaaagag caaattgtca
     2521 atatataaca aaaaataaac atactaagtt agtatgttta taaaaaatag tatacatggg
     2581 atactaatag catctagcaa aaaaccactt actaatggta caaccaaaaa ggcttttgga
     2641 gaatcaccat atttttgtgt caaagaactc atatttgcca aagcgttcgg agtagctcct
     2701 attccatgtc caatcaaacc acttattatg atggcagaat caaaatcttt tccaagtact
     2761 ctaaagcaaa taaatattga aaataggatt ataaagatta cttgaaaagt tactattaag
     2821 agaataggag gtaataatgt agataattca aataaatcta tattcataag tgataaagtt
     2881 aaaaaaattc ccaatgaaag ttcttttaaa aaatctataa gtttaaaatt taattctagt
     2941 atatttatct tatcatttaa atttctaaaa ataacagccg aaaacataca agttacaaca
     3001 gagggtaata gtatattaaa cgttatatag aagcatctac ttataatttc tcctgtattt
     3061 atacaaataa atatgagaag aacctgttcc aaaaatacag atggtgttat tgtttcggag
     3121 aagttattat tttttatcct attcttattt ctaaaaaatt tacttccaaa tctctttaga
     3181 tttctattat atctaggaat cgacaagtct atatttgatg atggttttaa tttatattta
     3241 tttattagaa atttagctac aggacatccc aatataccac ccataataag tcctattgtt
     3301 gcagcagata atccaacccc aactgctcct tctataccga gattttctat tgtaagtcca
     3361 aatgcagcag aataaccatg accaccttcc atagatacat ttccacacat aagccctaaa
     3421 agaggttcaa gttttataat ctttgatatt gtaatagcta atatattttg acaaaatgcc
     3481 aatactccac acaaaatcca atatctaaat aatacactac cccctttctt tataagattt
     3541 atgcttattt cgagtcctat agttgtaaaa aagaaggtta taaaatttgg cattaaattt
     3601 ccacttatag atatatctgc tatattaaaa tatttaaaaa ataaatttag aaaacaaaat
     3661 aatagacctc ctattactgg tgcaggtata cagaaattat ttaaaaagtt aactttattc
     3721 ttcaataaat taccaattaa aaagaagaat atagatagtg tcaaggtttg cactatatca
     3781 aaacttaagg taatcaaaat aagtcctcct ttgtaattca aagttttaat tatataactt
     3841 aaattcttat gtattgaaat tttattatca aatgtatatt aataaatgta tattaaaaag
     3901 aatcagtaga tataattaga tagtcataaa ttaaattaaa atattttaat gagatgtgac
     3961 ttttttagaa tgaaaaaccg tagtatattg atataataga aataagactt aaaaatctgt
     4021 tgatagtatt atttataagt acagtaggag gataaaatgt ttgaaaattt acctaagata
     4081 gatttacatt gtcacttaga tggaagtgtt agagtagaaa ccatgcttga tatagctatt
     4141 aaagagaaaa tagatttacc ttcaaataat atggatgaaa taaaaaaatt agcaaaggtt
     4201 tcttttaatt gtacttcact agatgaatat ttagaaaaat ttgatttacc cttaaaagta
     4261 atgcaatcta aggaaaattt aaaaagagtt acttttgaac ttttggaaga tgcaagtaga
     4321 gaaaatgtaa aatatattga aattagattt gcaccattac ttcacaccca aaaaggtatg
     4381 agtgttaaaa atataattga gggtataatt gaaggaatta gagaagctga aagtatttat
     4441 gatataaaag gcaatttaat attaggttgt atgagaacaa tgacctcaaa agaggcatta
     4501 ttagtaattg aagaagggaa atcatttgta aataaaggtg ttgtagcagt agacttgtgt
     4561 ggtccagaga aagagggatt ttgtaaggaa tataaagatg tttttaaatt agcaagagaa
     4621 tatggatata aagttacaat acatgcagga gaagctgcaa gtggagaaaa tgtattggat
     4681 gctataaata ttttaaaggc ggataggatt ggtcatgggg taaaaattaa agaccataaa
     4741 aaggcgtata atttagttaa agataaaaaa atattgcttg aattatgtcc aactagtaat
     4801 gtacaaacta agacagtaga ttcttatgaa gcacatccat tttatacttt ttataaagat
     4861 aatttacatg taagtataaa tactgataat agaactgtat cagatataaa tttaagttca
     4921 gaactaaatg ttatatttga tacttttaaa ctaggattag aagactataa aataatttat
     4981 agaaatgcag tagaagctag ttttgcagat aaagaaacta aagagtattt gaatagttta
     5041 atttaataga taaaaatatt taaactttaa attaagctat taaattgaca aaaatacatt
     5101 gtattaggag aaaaaatgtg aaagattatg aagatgttgt ttatattaaa ctagtcaaaa
     5161 aagaacttga agaatggaag aaaaatatat taaagaaacc ttctatattt gacaaagcat
     5221 caaagggtat acagaataaa tttaatggag tattgccaga taaatatcat gaaataatta
     5281 cttcttctat aaaagtaatg acaaaaaccg tactgtttgg atataaatat gctacaaagc
     5341 caccacttag aaatatgtca ttagaagaaa gaaaaaaatt agttttagaa aaagctaaaa
     5401 cttacaagac cacagcaatg attgaagggg caggaacagg ggcaggagga atcctcttag
     5461 gtttatctga ttttcctttg cttttaggca ttaaagtgaa atttttatat gatgtagctt
     5521 caatatatgg atatgatgtt aaagattata gagaaaggat ttatatatta aatatattac
     5581 agttggcttt ttctagtcaa cagcatataa acaaaacttt taaattgatg aataactggg
     5641 atgaatatgt aaatagtcta ccagaagaca tcaatgattt tgattggaga acatttcaac
     5701 aagagtatag ggattattta gatttagcta agtttttaca actaatgcct ggaataggag
     5761 catttgtagg gttttatgtg aatggaaaat taattgataa attatgtgaa actgctgtgt
     5821 ctgcttatag attgaggatt agagagctta agttataaga gaaattataa cataaaaaat
     5881 tagttataca atatatctat atgtatctaa ttttgcattg gtaaaaacaa ttatggataa
     5941 aaggtaaagc atgatagaat ctaatgtaga ggtttgtatt ttaatggaaa aatttagttt
     6001 ttattgctta agatacaaat tgaattacga agagaagtaa ttgggaggaa tattttcatg
     6061 acaaatttgc caaaacaatt tgatagcttc aatgaagcta gacaaaaagg atttataaat
     6121 gcaaaggaac ttaaggaaag tgggaaaaag atggttggag ttttctgtac atttactcca
     6181 gttgaaattc caatggcagc tggagcaact gtagtaggtg tatgcggtgt tagtgaagag
     6241 ccaatacctg atgcagaaag ggtgcttcca agaaatcttt gtcctttaat taaatcaagc
     6301 tatggacatg caataacaga tacctgtcct tatttctatt tttctgattt gttgatagga
     6361 gaaaccactt gtgatggtaa gaaaaaaatg tatgaagaac ttgcaaaagt taagccaaca
     6421 tatgtaatgc atttgccaaa tacttcaaaa ggagaatttg cctataaact ttggaaagat
     6481 gaaatgataa gattaaaaga agaggttgag aaatctttag gtgtaattat aactgaagaa
     6541 gatataagaa ctgctataaa agataaaaat gaagaaagag aacttttaaa agagttttat
     6601 gcacttggta aattacaacc atctgcactt acaggccttg agttacataa tgttttatat
     6661 caagcaggtt ttaaatttga ccgtgcagaa cttaaacatt ctttaagaaa agttatagat
     6721 gatatgaaag aaagatatga aaaaggtgaa tgtcctgttc aaaaagataa gccaagaata
     6781 ttgataacag gttctcctat tggtggaata agcgaaaaaa tagttaaaac tttagaagat
     6841 gcaggggcat cagtcgttgc atatgaactt tgtggagcta ttagatcaaa cgatttattg
     6901 gttgatgaag aaatagaaga tgtatatgat gctttaacac aaaaatatat aaatataggt
     6961 tgttcttgta tgatgaataa tgacaatcgt attgaattat tagatagaat aatagatgaa
     7021 tataatgtag atgctgtaat agatgtagtt ttacaagcat gtcatacatt taatatagaa
     7081 agttatagaa taagagaatt tgttacaaaa gaaaaaaata agccatttat gtctcttgaa
     7141 actgattatt caaaatcaga tactgaacaa ttaagaacta gatttgaagc gtttgtagag
     7201 atgttgtagg aataaataaa agtaggagga actatgttta gtataggagt tgactcaggg
     7261 tcagtggcaa caaagggtgt gctatttgat ggtgaaaaaa ttattaagaa aataattata
     7321 ccaactggat ggagccctaa aagtacatct aaacaggtct atgagttgct aagtagtgaa
     7381 attgacaaaa aagatataaa aaaggtggta ggtacaggtt atggaagggg agtaatggat
     7441 tttgctgata agaaagtaac agaaataact tgtcatacta gaggtattta ctttttaaac
     7501 aagaacataa ggactatttt agatgtaggt ggtcaagata gtaaagttat aaatttagat
     7561 agagatggaa atgtatttaa ctttataatg aatgataagt gtgctgcagg aactggtcga
     7621 tttttagaga ttacttctaa ccttttagga agcgatattg aaagtataga tacattagct
     7681 aaaggatatg aaccagttaa tatatctagt atgtgtacag tttttgctga gtctgaaatt
     7741 gtaagtcttc tagctcaaaa catttctact ggagaggtag cagcagggat acttaaatct
     7801 attgcaaata aatccacctc aatgttagca agaggtgagg taatagatga agttgcattt
     7861 actggagggc ttgcaaaaag taaagaacta gtaaaaatga tagaagaaat actgggtaaa
     7921 aaaattttta ttgcagaaga tactcaaata ataggagctt taggagctgc tgtaatagga
     7981 tttagataaa aacatataaa aagaaaatga gaagctgaat ttattataaa taaattcaat
     8041 tttttatttt attttattta tttataaagg aaaactttgg tataattgat ttaaatgatt
     8101 gtatatttgt aaatacttga tatttagtca tatataacat tataatttta aaaattagct
     8161 caaaataagg gggagaaagc gtgaaaaaat ttacttcaaa aaaagtaaca aggtttgttg
     8221 cgctgttttt aatatctatt ttagtaatgt caacaattcc tgtatcagct gacaatagca
     8281 atacattgaa tcagataaaa aaagcagaat acagtgagac gtataaacaa tatctaaatg
     8341 atgctaaaaa tggacgtaca gaaaaatata atgggattat accaaatcca tatcagttgg
     8401 aaggtactca aatccaaagt aaaggaagaa cagctccaac atcttatgac cctagaaaat
     8461 taggtcttat gacatcaata aaaaatcaag aggatttagg aatctgttgg gattttgctg
     8521 caatggcaac cttagaaagt ttcttaaagt taaataatta tggagattat gatttatcag
     8581 aagaacactt aagatggtgg gcttcagatg gtgaatatgg atggtctgta aatgatatgg
     8641 atggtgcttt aaattatgaa gctatgggat atttaacatc ttggtcaggt cctaaattag
     8701 aaaaagatat cccatacaat ggtagagtat ctaaagcaca aggagcaaaa aaaccaacta
     8761 atatgaatac agcacctact gtttttaatg tgacagatgc tgtgtgtgta agtaatgaca
     8821 taaattctac aaaaaatgct atattacagt atggagcagt aacatctggt tattatgaag
     8881 atattaaata tcaaagtgat gaccaaaatt catattattg tccaacaaaa agttttaata
     8941 caaatcatgc tatttcaatt gtaggttggg atgataacta ttctaaagat aattttaatg
     9001 caaatattag accatcaaaa aatggtgctt ggttaataaa aaatagctgg ggagattata
     9061 attctgaggg tggatatttc tggatatctt atgaagacaa gacacttatg tctgatatag
     9121 ataatttttc aatcaaaggt ggtaaaaaac caaatgatga tgagaagatg tatcaacatg
     9181 actatgcaag tattgtacct ttaatttcaa aaaagataac agctgcaaat gtatttgatt
     9241 tcaatagagg cgatgaaact ctaaaatctg taatgttctt aactgaaagt ataggagcta
     9301 agtatgaagt atattatgct ccagtagtaa atggtatacc tcaagaaaac aatatgaaaa
     9361 aattaaaaga aggtactgcc caatattcag gatacataac agttccaatt gattcttttg
     9421 atataccaga aggaaagggt gcaattgtag tcagcataga ggggaaaaat ggagagtcaa
     9481 ctataggttc tgagtcaaat gtaccaggat atgatacatt taaagcaaaa gctaatttag
     9541 gtgaaagtta tatcatagat aatactggaa aattctttga tataaataga gatagtaact
     9601 tctatccgtg taattttact ataaaagctg ttacagaaaa gtcttctggt gagagtatcc
     9661 caaatgagtc tttaattggt agtgatagat atgaaactgc tatcaaagtt agtcaaaatg
     9721 gtttcaattc ttcagaaaat gtagttttag taaatggcag ttcaatagta gatgcattgg
     9781 cagcaactcc ttttacatct gctataaatt caccaatatt attgacacaa aaagaagctt
     9841 taaattcaaa aactaaagct gagatacaga gattaggtgc taagaaagta tacctaatag
     9901 gtggagaaaa ttctataagt aaagaaattg aacaacaatt aaaaagctta aatatatcga
     9961 tagagagaat atcaggaagt gatagataca agacaagttt attattagct caaaagttaa
    10021 atggtataaa aaatgtatct caaatagcag ttgtaaatgg agtgaaagga ttagctgatg
    10081 caattagtgt aggcgctgca gcagcagaaa ataacatacc aatcatactt gcaaatgaaa
    10141 agagcgaatt gcaaggagct gatgaatttc taaattcatt aaatatagag aaatcttata
    10201 ttattggagg aacagcatcc ctatcaaaca atctagaaag taagttaaaa aatccaacta
    10261 gattgtctgg tattagtaga gacgaaacta attcaaagat aatagataat ttttataaaa
    10321 aagatacttt aaaaaatgca tttgtagtta aaaatggaat caagaatcaa aatgatttaa
    10381 tagatggatt ggcagttggt cctttaggag caaaaactgg gtcaccagta attttagttg
    10441 gtgataaatt agctgatagc caaaaagaag ttcttaaaaa taagacttta gaaaaagtaa
    10501 ctcaagttgg tggaggagca aataaaaatg catttaaaga gttagtaaaa ttaaaaagtg
    10561 ctaaatagag tacttaagta ttaacatata ttagtgtgta ttagcaactt atcttaataa
    10621 aagctgaaaa taattacttt gaattattga taaataagat aatcaatgat tgcaaagttt
    10681 tgttttcagc tttaaattta tgtagtataa gtttaaaaac ataaaaaagg gttactttat
    10741 atagaaatat agaaaatctt acattttata tataaataac ccatatttaa ttaacttttt
    10801 atagtgttct aatagatatt attgaactta atggtaaata taatgtgtaa gtttcattta
    10861 aagttggatt agattcatct atatatcttg agcaaagtgt aattgataag ctatctgctt
    10921 ctataatttc accatataga ggacaactgc taaagtaaga atgtacttca acatatttat
    10981 ttaagttttc ttgaacataa tcgtaaaaac ggttttttgt agtagtagcc ataaaatcaa
    11041 ctcctttatt ttttattaaa gttgaaactt ttaaaaagat aaatatgatt acttctatta
    11101 gaaactaagt taaaaaataa gcaaatagat atgaaaatta gaataatata ttatttatat
    11161 cttaattata acactagata ataaaaaatg ctatataaaa ttcagaattt tcgaaagttt
    11221 ttaagcttat ttttaaatat actaataata ctattgacat tagatagaaa aagtattata
    11281 ttgactatat aaattatttt agtcaaactg gaggaaaaag atgcctaaat catattctga
    11341 taaagaaaga gagtatataa taaaaagact taaagaagaa gcaagattgt gtatggagca
    11401 atatggtatt agaaaaacta ctgttgatga gcttgtaaaa cgggtaaaaa taccaaaagg
    11461 aactttttat ctattttttc aatcaaaaga attgctcttt tttgaggtat taagagatat
    11521 acatgactct atacaaaaag aaatcctata tgagataaat agagtagatg aaagtattac
    11581 atgtgaacaa ttgacagata tatttatgaa attctatagg atggttgata gtacttcaat
    11641 tttaaatctc atgataaatg gagaatttga gatattggtg agaaagttac cagattctat
    11701 aattgaagaa cactttagac atgatgactt tgaaattgga gagattatat cttgcatacc
    11761 aaatgcaaaa aataaagaca tagaaagctt tagtggagca tttagagccg tctttcttac
    11821 catgctatat aagcgcgagg ttggaagtaa ttgttttgaa gatgctttaa gattaatgat
    11881 aaacggatta gttatacaac ttatggaata gaatttaaat tttgtttgct aataactttt
    11941 attaaaaatt attaacaaac aaaaaaatta tattttattg accatatagt caaaatagtc
    12001 aaaaatttga aagggtgata ttgaatgatt aaagttgatg atttatcatt tagttataca
    12061 gatagagatt ttttacaaaa tattaacttt gaagtaggaa aaggggaaat actaggattt
    12121 ttaggaccat caggtgcagg aaaatctaca ttacaaaaga ttctaattgg aatgataact
    12181 aattatggag gaagtgtcat tgtaaatgga gtagagagta aaagacactc aaataaattt
    12241 tatgaaaata taggagttga ttttgaattt cctagtttat atgaaaagtt gactgcaata
    12301 gaaaatttaa aatattttgg gtctctatat tctaaaaaac tactatctat tgatgagtta
    12361 ttaaaatcag taggactaga aaatgaagca aataaaaggg tatctgagta ttcaaaaggt
    12421 atgaaatctc gattaaattt tataaaagct ttattacata atccagatat tttattttta
    12481 gatgaaccaa caagtggtct tgacccatca aatagcaagg ttatgaaaga tataatttta
    12541 tcagaaaaat cgaaaggtaa aactattatt ttaacaactc ataacatgct agatgcaaca
    12601 gagctttgtg acagagtagc gttcattgta aatggtaaaa tatctgcttt agatacacca
    12661 cataatctta ttatgtcaaa aggagctatt aaagttagat atacttactt tgataatgga
    12721 gaaaaaactt ctgaatgttt tctgaacaat actgctaatg ataaaaattt gaatatgctt
    12781 attgagaaaa ataaactttt atcaatacat agtagtgaac caacattaaa tgatattttt
    12841 attgagataa cagggaggaa tttacaatga ggctaaaaaa tcttatttta ggagatataa
    12901 agtttcaatt taagtatggt ttttattttt tatatctttt tttaagtatt atatatattt
    12961 gtattatcaa tgtatttcca acttttatga gagaaaaaat agctataatt atgatttatt
    13021 ctgacccagc agcaatggga ttgtttttta tgggagctat tgtacttctt gagaaaagtc
    13081 aaagggtttt aaattcctta gctgtatctc cagtaaaagt atctgagtat attttatcaa
    13141 aggtaatttc attaggagta atatctagta ttgtagctat gtttattgct attacattaa
    13201 atttagataa tattataata tcaactatag gtaccttttt ttcttctatt atattttctt
    13261 tgcttggatt gataattgca agtaaagcat ctagtttgaa tcagtttatt gttttaagta
    13321 ttcctattga gattatatgc tttattccac caatactgaa tgtacttctt gacacaaaat
    13381 catatgcaaa tttgtaccct tttaatatat gtatttctct tattagtgga gataaaaatt
    13441 ttataatgat taatatttta attttaatat caattataat aataatttat tttataactt
    13501 attattttat ttgtagcagt tggaaaaagg taggtggagt aaaactatga taaagattat
    13561 aaatgcgttt aaacaagagt ttgaacaaat taagagagat gctatgcttt ttatagtatg
    13621 tgtttctcca atattatgtg gagtatttat aaaatttgga ataccattaa tacagaatat
    13681 atcacttaac aagttttatt atcaattaaa tttagagccg tatttcttga tgtttgattt
    13741 acttttagct tttattacac cttttatgtt cttttttgct tctacaatgg tcatattggg
    13801 agaaattgat gacagcattt caagatattt aatagtaact ccgcttggta aaactggtta
    13861 tcttatttca cgatttggaa taccaggtat cttagcattt attattacta tgattctact
    13921 tatctttttt tctctaacta aaatttcttt cttgttaaac ttatcaattt ctttattagt
    13981 tttattacaa ggagttatta tatcaatatt agtgatttct ttatcatcaa ataaactaga
    14041 aggaatggta attacgaaat tctcaggtgt ttttatgatg ggtattttag caccattttt
    14101 tattttaaat aaagcacaat atattctgtt cttcttacca actttttgga tatcgaaggc
    14161 atttaaggaa gataattata tctatatgtt tatttcaatt gttatatcat taatatggat
    14221 tttattatta tttaaaaagt ttagtaaaaa aataattaaa taagaaaatt taatatataa
    14281 atgtaaggtt gacttgacag taatgttgaa aaatgttaca tttaagatgt aaagttaacc
    14341 ttacatgaat ggagcaaata tgaaaaataa aataaagata ttaagagaaa aattaggatt
    14401 tacacaagaa caactaggaa gattagttgg aacatctaga caggctataa atgctataga
    14461 aactggtaaa aatgaacctt caatatggtt ggcatatgat atttcaagaa tttttaatga
    14521 agcaattgaa agtgtatttt tatttgaaga aagtgaaaga aaatcaagag cacagataag
    14581 taggggagaa tattatggct ataagaaaga taagaacatt tgatgatgaa attttaagaa
    14641 aaaagagtaa gtatgtagaa aacgtagata ataagataag agaaatatta aatgacatgg
    14701 cagagaccat gtacaatact ccaaatggag gaggattagc tgcatgccaa gtaggagtat
    14761 taaagcgatt agttgttata gatttaggag aaggacttat taaacttgtt aatcctgaaa
    14821 ttattaaaca agaaggggaa caaattgttg ttgaaggatg tttaagtttt cctgaagtat
    14881 ggggtaaatt aaaaagacca aaaaaagtta cagtacaagc tctaaacgaa tacggtgaga
    14941 aaatagaaat taaaggttca ggttttatgg caaaatgtct ttgtcatgag atagaccatt
    15001 taaatggtat agtttttaca gataaaatta ttgaacatgt aaaattgtaa gatagattgg
    15061 ttattgaatt tataaaatat tttatagtaa ggagttaaat tatgagatta tggcataagg
    15121 atttaattga tgtattacct aaaaatcaat tagtaagtca atggagagag ctacttgcta
    15181 taaaaggttc tatagataaa aaaggtactc caaaccatct attagtgaat aaagtattga
    15241 attatagtat agatgaattt aaattttata caaaaattgt acatgatgaa atgttaaaaa
    15301 gaaattataa acctaatgaa cttaaatata caagtatatt aaaatggaaa aatagaaatt
    15361 ttgcaaatga tatatctaat gaacatagtt taaatttaga aaatttatat gatgattggc
    15421 ataataaaat gtatttaaag caatgcttat ataatttaga agaaaaagca acatgtggag
    15481 gaataccaat aaatgaatgg aatatattac tttgtaaata tagtaaagat tatgaattgt
    15541 ggtctggaaa tatcatgttt taattactat agattaacat tatagtatta ttgtatataa
    15601 agaggtgatt atttgtataa taaaataaaa tatattccaa taaattgtga aatagcttgt
    15661 aataaattaa aaaggagtat gccatataaa tgggatttaa atatttatcg tggatgtgag
    15721 catggatgta aatattgtta tgctatttac tctcataagt acatcaactc caataattat
    15781 tttgaagaga tttatgtaaa aacaaatata gtagaaaaac ttgagagaca acttaaaagc
    15841 agtaaatgga agaaagaagt aatcaatata ggaggagtta cagatagtta tcaaccaata
    15901 gaagctgact ataaaattat gccagaaata ttaaacctgc tgataaaata caagacacca
    15961 gctattattt ctacaaagtc agatttaatt ttaagagatt acgatttaat tgataagcta
    16021 tctaggatta catatatcaa tgtagcatct acaattacca cagtaaatga agatacacaa
    16081 aggcttattg agccaaatgg tgtggattca atgagacgat ttaaaatgtt aaaagaattt
    16141 agaaaaacta acgcatcagt tggtctacat attatgccaa ttataccata tattacagat
    16201 gattttgata atataaattc gctatttaga catgcaaaag aatgtaatgt acattatgtt
    16261 ttacctggga ctttatactt aaggggtata actagaggtg ttttttttga ctttgttaaa
    16321 aaagagtttc ctgaattatt tgataaatta tcaatactat atagtgcagg ttcacttaat
    16381 agggagtata aaaatcaatt gtataaaatg gttaatgaac ttagatataa atattctcta
    16441 tcaagcagtt actctaaaat aatgaaggaa aagttaaaaa actcaggaga tgtacagata
    16501 tctttttttg attagtttta tataaaaaaa ttttttttac tatgatgatt aaaatttatt
    16561 aaaattaaaa tttattgaca aatataaaat ttaaatat
//
LOCUS       DAEMHW010000077        16586 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEMHW010000077 DAEMHW010000000
VERSION     DAEMHW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.077"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            760..2430
                     /locus_tag="KPK67_002081"
     CDS             760..2430
                     /locus_tag="KPK67_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBG2459049.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2552..3286)
                     /gene="pflA"
                     /locus_tag="KPK67_002082"
     CDS             complement(2552..3286)
                     /gene="pflA"
                     /locus_tag="KPK67_002082"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBG2459050.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGIDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3446..5677)
                     /gene="pflB"
                     /locus_tag="KPK67_002083"
     CDS             complement(3446..5677)
                     /gene="pflB"
                     /locus_tag="KPK67_002083"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBG2459051.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLASATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5970..7031
                     /locus_tag="KPK67_002084"
     CDS             5970..7031
                     /locus_tag="KPK67_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBG2459052.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7122..8735
                     /locus_tag="KPK67_002085"
     CDS             7122..8735
                     /locus_tag="KPK67_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2459053.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9066..11378
                     /locus_tag="KPK67_002086"
     CDS             9066..11378
                     /locus_tag="KPK67_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2459054.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDEFDTTSNKVNYISDELD
                     TTSNKVNYILDELDTTSNKVNYVSDELDMALHKVNCVSDELDITSNKVNYVLDELDIT
                     SNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCF
                     DMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFME
                     LAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKFET
                     PSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKK
                     SIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHG
                     NPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            11466..11801
                     /gene="spoIIAA"
                     /locus_tag="KPK67_002087"
     CDS             11466..11801
                     /gene="spoIIAA"
                     /locus_tag="KPK67_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBG2459055.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            11815..12243
                     /locus_tag="KPK67_002088"
     CDS             11815..12243
                     /locus_tag="KPK67_002088"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBG2459056.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            12266..13030
                     /gene="sigF"
                     /locus_tag="KPK67_002089"
     CDS             12266..13030
                     /gene="sigF"
                     /locus_tag="KPK67_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBG2459057.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            13310..13747
                     /gene="spoVAC"
                     /locus_tag="KPK67_002090"
     CDS             13310..13747
                     /gene="spoVAC"
                     /locus_tag="KPK67_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBG2459058.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            13914..14927
                     /gene="spoVAD"
                     /locus_tag="KPK67_002091"
     CDS             13914..14927
                     /gene="spoVAD"
                     /locus_tag="KPK67_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBG2459059.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            14941..15303
                     /gene="spoVAE"
                     /locus_tag="KPK67_002092"
     CDS             14941..15303
                     /gene="spoVAE"
                     /locus_tag="KPK67_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBG2459060.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVIFTPKAKP"
     gene            15486..16502
                     /locus_tag="KPK67_002093"
     CDS             15486..16502
                     /locus_tag="KPK67_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2459061.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLNTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
BASE COUNT         6441 a         1944 c         2776 g         5425 t
ORIGIN      
        1 gtatttttta ttaattctta caaaatcaat gaaaataagt atatccattt taaagtacat
       61 aaatttaaaa atccagaaaa atatacttaa attatgtaag taaaagaggg gttgaatcag
      121 ttgaaaatgt aatattagta aatagtacat caatcctata taccctgtaa gcaactctat
      181 tttcatgtac taaaaatgta cctattttat taactgaaaa gaataaatta aatgtaagta
      241 cataagaaga tagaaaaaat taaaaacaaa aaatatttat ataaataaat ataaaaaatg
      301 atataatccc tattaaaaaa tttatagatg atgaagaaat atcaaaatct tatattatag
      361 gaagagacac ttctttgcca aaatctatag aaaagcaata ccaaatccaa agagaatagg
      421 cggagaaaaa aataaaataa attcaagagt aatagaaaag ttttatagaa taaagaactg
      481 ataactcttt atataacgaa aaatagaatt ttaagagaaa atgatttaat aaatgattta
      541 acatggtgtt ttagcaggta aaaataaatc accagtaatg attatagaaa aattacttta
      601 tgaatctcaa aaaacttagt taagagtaag agatttgata agataactaa agttggggaa
      661 atgtgtttga tgagataaaa aattagtaaa atagtataat ctaaataagg atattttgtt
      721 gattaaacta cactaatagt aatattgtag ggggaaaaga tgagagaaga atcgaactct
      781 ttaagtttag accatataga aattttcttt gaacttttat ctcaaagtac agaagattat
      841 atattttttt gggatataaa taggaataaa tttaaaattt cttcagcaat ttttgatgaa
      901 tttaacttaa gtaaagagat agaatctgat ttagtcaatt gttggagtaa aattgtttat
      961 ccggatgatg ttcaaatatg gaaagatgat atacaagaat tactacatgg aaaaaaaggt
     1021 gagcacaatt tagaatatag gctgattaat aaatatgaag aaatagtatg gatttcttgt
     1081 agaggtaaag tttatgtgtc ggatgaccca aaaactatat ttctagttgg gagaataaag
     1141 aatattggtg aaaaaaataa atttgattct atcacaggaa cgtggaatag agaacaattt
     1201 gaacatagaa tgaattatct tataaaagag aaaatttata aaaatggtgc tatgtttatt
     1261 atggatattg ataattttaa aaatataaat gaaaaatatg gacattctta tggtgacaaa
     1321 gtattgcgtg caattgctac tgaagtatta gaatatcttc caaaagatgt aagattgtat
     1381 aggttagatg gagatgaatt tgcatttttt taccctatgt gtactaaaga aactatagag
     1441 aaaatatatg aaaagataca gatgtataca aatactcagc atgaaattga atcaaataag
     1501 tactattgca cagtgactgc aggagttgct atgtatccag aagatggaga taattattta
     1561 gatttattta aacatgctga tattgcattg gatatagcaa aaattagtgg taaaaataga
     1621 attaagtttt tttcacaaga gctctatgaa aataaactaa aggtaatttc aatgcaacaa
     1681 aaactaagag agtgcgttga aaataatttt aatgactttg agctgttttt tcaaccccag
     1741 gtaaatgctg ttacaaaaga ggtaataggg gcagaggtac ttttaagatg gcattcgagt
     1801 acttatggag aagtatctcc tgttgaattt ataccaatac tagaacagag taatttaatt
     1861 ataccagttg ggaagtggat tataaaagaa gctgtaaaac agtgtaaaga atggcataaa
     1921 ataaatccag actttaaaat atctgtaaat gtatcgtata ttcagttaaa ggaagatttt
     1981 tttagagatt ttatagtaga atgtttagta gagtatcaat taagacctga atttttaatt
     2041 ttggaattaa cagagaattg ttggatacca gatataaatt tactaaatga taaatttatt
     2101 agtttaaagg gtataggagt atatattgca atagatgact ttggtacagg atattcatct
     2161 ttaaattacc ttaaagaact gtctgttaat ataataaaaa ttgaaagaag ttttgttaag
     2221 aatattacat ataatagtta tgaatatact tttcttgaat atattattaa attagctcat
     2281 attataaatt taaaagtttg tgttgaggga atagagtctt atgaagagta tgatatagta
     2341 aaaagcttag gtgtagatat tattcagggt tttttatttg gaagacctgt aagtgcctct
     2401 gaattttata aatttcagct atcaaagtga tgtatataaa atgagatata tagtatataa
     2461 aaatagggca aagtggaagt tttgaagttt attaaaaatt tacactttac cctattttgt
     2521 tatgtcttct ataaaggatt aatatactac actaactatt atatactttc actccaaatt
     2581 tagagattat ttcactagct ttttggacat cttccttagt ggcatcagga acatctttta
     2641 gttcataatc gatacctaaa ctttcccatt tgtgtattcc catggaatgg tatggtagca
     2701 attcaaatct ttctacattt ttcaatgtag ctacaaattg acctagtttt tctaaattat
     2761 ctatatcatc agtaatgcca ggaactaaaa catgtcttat ccatacagga atatttcttg
     2821 attctaagtg tctagcaagt tttaatgcct tatctatacc aacaccagtc aaatcaatag
     2881 atttttcctc aaccatatgt tttaaatcaa gtaaaaccaa atcagtatta tctaaaactg
     2941 ggtctatagt ttctatatct acaaatccag atgtatccaa gcaagtgtgt atatcatttt
     3001 cttttgcttt tttaaataat tcacttaaaa attctggttg aagagtggat tcaccaccag
     3061 aggcagttat acctccacca gaaaacttca taaaagaagt atacttaaga gcatccgtta
     3121 ttatttcatc aactgtgtat tcttttcctg atttaatatc ccatgtatcc ctgttatgac
     3181 agtatttgca tctaagagga cacccctgaa aaaataatat atatcttatt cctggtccat
     3241 ctacagtacc aaacgtttct atagaatgta tctttccctt aatcatagag ttcacctcac
     3301 aatatatttt aataacctta tcaatagtta taatatttct ctaaaaaagg ccatcttata
     3361 ccagaaataa ttcaaatata agatagcctt ttatttttat tttttacaca gtagtaatct
     3421 tagttaaagt ataaaaattt aaagactaag ccattttacc gtggaatgtt ctgtttataa
     3481 catctaattg ttgctcttta gttaatttta tgaagttaac tgcatatcca gatactctta
     3541 tagttaattg tggatattct tctggatgtt ccatagcatc ttctaaagta gctctatcaa
     3601 atacatttac atttaagtga tgagcatttt gagcgaagta tccatccatc attgcagaaa
     3661 gattgtttat tctttcagtc atatcttttc ctaaagcagc aggtactata gagaaagtat
     3721 tagatatacc atcttgagca tgttcatatg gtaatttagc aactgaagat aatgaagcta
     3781 aagcaccatt agtatctcta ccatgcattg ggttagcacc aggagcaaat ggagcaccag
     3841 ctcttctacc atctggtgta ttaccagttt tcttaccata aacaacattt gaagttattg
     3901 taagtataga ttgagtagga taagaatttc tataagtttt attctttctt attttattca
     3961 taaagctttc aactaactct acagctatac tatcaactct atcatcatta tttccgtatt
     4021 ttggataatc tccttctatt tcaaaatcaa cagctattcc ttgttcattt ctaatagttt
     4081 taaccttagc atgttttata gcagataatg agtctgcaca aactgataac ccagccatac
     4141 cacaagccat agttctaaat atatctctat catgtaatgc catttctaat gcttcataag
     4201 aatatttatc atgcatataa tgtattacat ttaaagtatt tacatataat gttgctaacc
     4261 aatcagtgaa tggttcaaat ttactcataa cttcatcata atctaagtac tcagaagtta
     4321 taggttcaaa tcttggtcca acttgaacac cagatttctc atctacacca ccatttatag
     4381 cgtataataa agttttagct aagttaactc tagctccaaa gaactgcatt tgtttaccaa
     4441 ttctcattgc agatacacaa caagctatac catagtcatc tccccagtat gctctcatta
     4501 aatcatcatt ttcatactgt actgaacttg tatctataga tactttagaa cagaaatctt
     4561 tgaatccttg aggtaattta gttgaccata gaactgttaa gtttggttct ggagaaggcc
     4621 ctaatgtata tagagtattt aacattctaa atgagttttt agtaactaat gttctaccat
     4681 ctattcccat acctcctata cactcagtta cccaagttgg gtctccagag aataggttat
     4741 tgtaatcagg agttcttagg aacttaacca ttcttaattt cataacaaaa tggtccatat
     4801 actcttgtaa ttcttcttct gttacgattc cagcttttaa atctctttca aagtatatat
     4861 ctaagaaagt agatgttcta cctaaagaca ttgcagcacc attttggtct ttaacagcac
     4921 ctaaataacc aaagtataac cattgtacag cttcttttga gttagttgct ggttttgaga
     4981 tatcaaatcc ataagattca gccatttttt taagttcatt taaagctgat atttgctcag
     5041 ttatttcttc tcttaatctg ataacttcct catccatgca agaaacttct aatgattttt
     5101 tttgctcatt tttatcttct attaaagcat caacaccata tagagcaact cttctgtagt
     5161 caccaattat tctacctcta ccataagcat ctggaagacc tgttattata ccagattttc
     5221 tagcagctct catttctgga gtataaacat caaaaactcc ttggttgtgt gtttttctgt
     5281 atttagtaaa tatatctttt atttctgggt ctaattcata tccaaaagct tcgcatgaat
     5341 tttcaaccat ctttatacca ccatatggca tgacagctct ttttaaaggg gcatccgttt
     5401 gtaacccaac tattgtttct aattctttat ctaaatatcc tggagcatat gcagctattc
     5461 cagaaacagt ttttgtatca acatctaaag taccaccatt ttctctttct tttttaaata
     5521 ggaccattgc ttcgtcccat aattttttag tattttcagt ggcactagct aagaaagagt
     5581 catttccctc atatggagaa tagtttaatt gtataaactc tctaacattt atttctttag
     5641 tccatcttcc tgttttaaat ccttgccatg cattcattaa gtatttcctc cttagattta
     5701 aatgataaat aatactatta aaattaattt ttatatactt aatttatttt atatattttt
     5761 ttaaaaatat attttctaat ataataatag catattattc gtatcctgta tacataaaaa
     5821 ataaaaaatt gtcgctttaa agtataaaac agattatatg ttaataatgt ggtgaaatta
     5881 tacccatata taaataaaat aaaacaaata aatagctaat taaggagaaa atatttttta
     5941 ttttttgttt aggataaaga aattttatta tggaaaaaag aaaaagcata catcttcaca
     6001 aactgaatat ttatttaata gggggaggtg atagattgtt tatcacagtg gtattatttt
     6061 tagtaggttt cctattaatt actaaaggcg cagatatctt tataaattgt actgtagaga
     6121 taggtaaaaa aacgaatata tcagaaatta tacttggagc tactatagtt agttttgcaa
     6181 caacattacc tgagtttaca gtgtctctac ttgcttctat tgatggtcat actactatga
     6241 gtttaggaaa tgcagttggt tctataatat gtaatacagg gttagcatta gggttggttg
     6301 tttttataag tccatttaat gtagataaaa aaatgttttt ttctaagtct ttactattaa
     6361 tagtatccgt tatagtttta attttattga gtttagatgg agttataaca agaggtgatt
     6421 cattattact tataataata ttaatctttt atatggttaa taattataga agtgtcgttg
     6481 gaaaatctga tactaaaaat agaaatatta aaaataacac tattaaagac atatctacta
     6541 aaaaagggaa aaattataga ggtttttccg tattagaaat tgtaaaaata ctattacttt
     6601 ttgctacagg attgataatg atgataatag gttcacaaat ccttatagag agtggagtaa
     6661 taatagctag ttttttaaat atacctcaag gtatagtaag tttaactata atagcacttg
     6721 gaacttctct gccagagata gtttcgtcga ttactgctat aagaaaaaat catcatgaaa
     6781 tatcagtagg aaatattttg ggtgcaaaca tattgaatat agtatcagta atagctgtat
     6841 cagcaatacc aaataacata ccaatactat ctcaaaatag acaattggat ataccattca
     6901 tgatattatt attattaata gtaataatac caacacttaa atctaataaa cttagtagaa
     6961 tacaaggtat attgatgtta ttcacatatt ttctgtacat atcaattttg tatttcatgt
     7021 atattattta actatactgt ttaaaatatt aattgcagaa tatcctaaaa aagtatataa
     7081 tggataggtt aagattaatt gataacggag aggaataata tatgaatata acaaagtttg
     7141 aagatttacc aataagtgag ggaataaaaa aagctatagc tgaaatgggg tttgaagaac
     7201 catctcctat acaagcacag tctatacctg cgattttgtc aggtaaagac gttataggac
     7261 aagctcaaac tggtacggga aaaacagctg cttttagtat ccctatacta gaaacaatag
     7321 acccaaataa tagaagttta caagctgtag tgctttgtcc aacaagagaa ctggcaatac
     7381 aagtatcaac agaaataaga aagttagcta agtattcgca cggaataaag acattaccta
     7441 tatatggtgg tcagccaata gatagacaaa taaaatctct aaagagtggt gtgcaagttg
     7501 taataggtac accaggacgt acaatagacc atatcaatcg taaaacatta aagatggata
     7561 atgtaaaaat gattatatta gatgaagcag atgaaatgct agatatggga tttagagaag
     7621 atatagaaat gattttaagt aagattccag aagaaagaca aacaactttc ttttcagcta
     7681 ctatgccaag aggaattttg gaattaacta agagatatca aaaagaccca gagcatataa
     7741 aagttgttag aaaagaactt acagtttcaa atacaaagca atactatata gaaactaggt
     7801 catcaaataa attagaagta ttatgtagat tagtagatgt atatgaccct aaactatctg
     7861 ttgttttctg taatactaag agaaaagcag atgaattggt aggagattta caagcaagag
     7921 gatattttgc agatgcatta catggtgact taaaacagac tcaaagagat atagtaatgg
     7981 ataagtttag aaatggtaca atagatatac ttgttgctac tgatgttgca gctagaggta
     8041 tagatgttga tgatgttgaa tgtgtattca actatgactt accacaagat gaagagtatt
     8101 acgttcatag aattggtaga actggtcgtg ctggtagaga aggtatgtca tttacttttg
     8161 tatttggaaa agaaatgaga aagatgaaag atatagaaag atatactaaa tctaaactaa
     8221 taaagcataa tataccaaca ataactgatg ttgaagagaa aaaagtaggt actttctttg
     8281 cacaagttaa acaaactata gaagaaggtc acttaactaa acaattacaa tggttagaag
     8341 gtttctgtaa tgatgaagat tatgcaatgg ttgatatagc tgctgcactt gtaaaactat
     8401 ctctaggtga agaaatgaaa gaagaaataa tagaagaaaa acctagaaga gagcgtggag
     8461 atagaaaagg tggtactgga gctaaagatg gtatgattag attatttatc aatattggta
     8521 gaaatcaaag agtacaagca aaagatatag ttggtgctat agctggagaa gttggaatac
     8581 caggtaaagt agtaggtact atagatatat atgataagta tacttttgtt gaaataccta
     8641 aaaaagatgc aaaaacagta attgaaaaga tgaaagatat taagataaaa ggtaataaga
     8701 ttaatataga aaaagcaaat aagaagaaaa aataacgtat agtatacaaa actataatta
     8761 gaaaatatat aaaataatat attggatatt taaaaagcct gatgtgtttt caaaacagat
     8821 agtagataat ctgtaagagt taaatgcata aggcttttct tgttttatat aaggaatctg
     8881 aataacttac tgttaaatct ttgaattact gcacctcatt tacgctaagg atggagtatt
     8941 ctacctattt ttgataaaat atatctatgt tttatatact atagttttga acatagaaag
     9001 tttaataaat tatagtatat atgttaattt ttaataaggt aagaaagtag aaagaaggat
     9061 attttatgga tataataaaa aaattaagtc atgggtgtac attagattgt catgactgtt
     9121 gtaaattcaa tgtttatact aaaggaaata atgtagtaaa aattgaagga gataaaaatc
     9181 atccttatac aaaaggtttt atatgcaaaa aagggatggc ccatttagat agattaaatc
     9241 ataaagatag aataaagact cctatgctta aagttgatgg agtatggaaa gagatttctt
     9301 ttgataaagc tatagaaata atggcagaaa agcttacata ctataaagaa aagtatacat
     9361 caaagtctgt catgcattat gaccaatatg gaagtgggtc tgtgctaaaa tacataggag
     9421 atatattttt taatttttat ggaggtgtta gcagacataa gggtggtccc tgttggagtg
     9481 cagggatgca tgctcaaaaa tatgattttg gagtagctaa gagtcatgct atagaagata
     9541 tgttaaatag taaaagtata tttgtttggg gaaaaaatcc agcatatact acaattcata
     9601 ctatgcaaat aattaaaaaa gctaaagaaa aagggataaa gatagtagtg attgacccca
     9661 tatatacaaa aacagctcaa atagcagata agtatgtaca agtaaatcct ggaacagatg
     9721 gagctttagc tatagctatg gcaaaaatta tagtagaaga taaattatat gatgaagaat
     9781 atataaactc atatgttatt ggttttgaag aatataaaaa atatttatct tcattggaat
     9841 taagtttttt aattgatgaa tgtggtgtta aagagaatga tataagagag ttagtggatt
     9901 tatatactaa caaatattca agtataaatg ttggatatgg gcttcaaaaa tataaaaatg
     9961 gtggaaacac aataagagct atagatgctt taggtgctat aactggtcaa ataggattta
    10021 gtggtggagg ggtaaactat gctaataaag tttatccgag tgttataaat tctgaccctt
    10081 acaatagcca atcatatggt gaagataggg agttttatgt aagtaatatc agtaaattta
    10141 tagaagaatc tttaaaaaat acatcaaata aagtaaatta tgcatcagat gaatttgata
    10201 caacatcaaa taaagtaaat tacatatcag atgaacttga tacaacatca aataaagtaa
    10261 attacatatt agatgaactt gatacaacat caaataaagt aaattatgta tcagatgaac
    10321 ttgatatggc attacataaa gtaaattgtg tatcagatga acttgatata acatcaaata
    10381 aagtaaatta tgtattagat gaacttgata taacatcaaa taaaacagat tatatatcaa
    10441 atgaattgta taatttatct aataagtcaa tcaaagataa tattcctata aagatggctg
    10501 tgataactaa aagtaatatg ctgaatcaac tgccagattt agtagaattg gagagggtgt
    10561 tttcaaaaat tgaatttaaa gtatgttttg atatgtttat aactgataca gctactttat
    10621 gtgacatgtt tataccatgt acaaatacac tagagagtga agatataatt tatagctcaa
    10681 tgaccaatcc atatataacc tataatgaaa gagctgtaaa gccagcacat aaattgatgg
    10741 atgaatatta cttctttatg gagctagcta aaaagatggg attaaatgat tatccttttg
    10801 tagaaaagag aacttattta gaaaaagtga ttgaaccact taagcgtttt gacaaaaatc
    10861 tggatataga aaaacttaaa aacaattact ttactataca taaccctgtt gcttgggaag
    10921 ataagaagtt tgagactcca tctggaaaat acgagcttta ttcagaaagt ataaaaaact
    10981 tgggaataag tccaacacct gtatatataa gcaataaata taaagaaata gaagataaaa
    11041 atatttcttt tagattatta actaatcatc atgcagatac tttatttagt cagcatttta
    11101 tggacaaaaa atctatagct caagcatata taaatcggag aatggctaaa aaagtaggta
    11161 ttgaggacaa agatattgtg atattgaggt caaaaaaagc aaaaataaat gtacaaataa
    11221 atatagatga tggagtaggt aattatatag taaaaatgta tgttggttgg tggaagaaac
    11281 atggaaatcc taatagttta acagacactg gtatatcaga ttttgggggt caggtaacat
    11341 ataatgaaag tatggtagaa ataatacgac aaaactagtt aaatttcatt gaaggaataa
    11401 aaatataatt atagaattga ttaaaagtta ctatttttac aaaactattg aaggagggat
    11461 ttttgatggt aaattattcc ttagaacata agaatctata catagaattt atgtgctcag
    11521 aactagacca ccatgtagcc aatgaaataa gggaagaaat agataactta ttaagtgtaa
    11581 atcaagtaaa aaatgttgta tttaattttg aaaatattaa ttttatggat tcttcaggta
    11641 taggcgtcat cataggtaga tataaaaaga tttctaatga agggggacga gtttcagtaa
    11701 ttaatataag ttcacgagtt aagaagattt ttgatttatc tgggttaaat aaaattattg
    11761 gcatttatga tacgtatgaa gaagctctaa gttctttgta gggaggagta tgtgatgaat
    11821 aatattatgg aagttaaatt ttcggcaata tctgaaaatg aaagtttagc aagagttata
    11881 gtagcatcct ttgcagctaa gttagaccca acagtagatg agttagtaga tataaaaact
    11941 gctgtctctg aggctgttac gaatgctata atacatggat atgaagaaga cagttctaag
    12001 tttgttttct taagatgtga gatagaagga aatactataa agattatagt tgaagatgaa
    12061 gggtatggaa tagaaaatgt ggagaaggca atggaaccac tttatacatc aaaaccagaa
    12121 cttgatagat caggaatggg attcacagta atgaaaagct ttatggacga tgtagaggta
    12181 agttctgtaa aagataatgg gactagaatt gaaatgacta aaaaaataaa tgtgcctaaa
    12241 taaagtccaa aagtgaggtt ttattatgga agtaactgtt gccagagaag aaaaaaaacc
    12301 tcttcttagt catgaagaga ctctagaact tattgaaaaa gttcaaaatg gagatgaaga
    12361 ggcaaaagaa atattaatat caagcaattt aggtctagtt aggagcgttg tatctagatt
    12421 tttaaatata ggatatgata gagaagattt atttcagtta ggttctatag gcttaataaa
    12481 atcaatatat aaatttgacc caaaatttaa tgtaaaattc tctacttatg cagtaccaat
    12541 gattttaggt gaaataaaga gatacttaag agatgatggt atgataaaag tatcaaggtc
    12601 gttaaagcaa atagctgtaa aggcaaagat ggaaagtgaa gctttaacta aaaaattagg
    12661 tagagaaccg agtatagaag aattagctaa agcaatagat gtagaaaaag aagatttagt
    12721 aatggctatg gaagctaatt tcaatgttga gtatttgcaa ggagttattc atgaagagga
    12781 aggttctcca atatgcctta ttgataaaat aagcatgaaa ggagaaagtg aagaagaaaa
    12841 agtagtggat aatatattgt taaaagacat acttggaaga ctggataaga gagagcgtca
    12901 aataatagtt ctcagatact ttgaagacat gactcaaagt gaaataggag agatgctcaa
    12961 tatatcacaa gttcaagtat ctaggataga gaagaaagta ttgtctaagt taaaagaata
    13021 tatatcgtga taatacttat gatatgtaga ataacaaaat ataataaata tattactaaa
    13081 ataaaatgta ataacttttt atgttaagtt tttaaattat taaaagatat taaaataata
    13141 aaaagcatta aaaaagtaaa aatataaaaa caaggtgtct ttatggaaaa atagattttt
    13201 tctttattag acatcttttt tattttttga ccaaacttta gatagtcaaa ataaataata
    13261 ggtttatttt tttaaaatta acaaataata ctttagagag gtgtttagta tggataaaaa
    13321 ttataaaaaa tatgtagacc aaataagccc aaaacctaca tatttaaaaa actatacact
    13381 tgcatttatt gtaggtggaa ttatttgtat gatagggcaa gcaataaatg atttgtatat
    13441 gaaagttgga ggtcttgata aactaggtgc tagttctgca acatcaataa cattaatatt
    13501 tataggggca tttttaactg gattaggagt ttacgaccta ataggaaaaa gagctggagc
    13561 tggttctata attccaataa caggttttgc taactcaata gtatctccag caatggagta
    13621 taaaagagaa ggctatgtat taggtgtagg tgcaaactta tttaaaattg caggaccagt
    13681 tttagtttat ggtataggtt cttctatttt atgtggaatt atttattata tttttaagat
    13741 gttttagatg atttttattt aatttaaatt ttattaatta tgaagaaggt ataaaatctg
    13801 aaaatagcta ctaaagtaac taattgaaaa ataattgtgc agatttaatt aatattttta
    13861 attaatatta aaaatttgaa tagtaaaatg caaaattaaa aaggtgtgaa aatatgaaaa
    13921 ataaaagaat tggaaaaaga acagtcaagt tagaaaataa accaaccata atatcaactg
    13981 gaactatagt aggtcctaag gaaggtgaag gaccactaaa ggactatttt gatatgatta
    14041 tgactgacga tttatatggt gaaaagactt gggaattagc tgaaagtaaa atggtagaga
    14101 ctgcatctca acaagctatc caaaaggcag gtaaaaaatt gtcagatgta aattatatgc
    14161 taggtggcga tttaataaat caaatagtgc cagcatcatt tgcagcaaga gaactggcta
    14221 ttccattttt aggtatatat ggagcttgtt ctacaatggc agaaggtctt tgtataggtt
    14281 ctatgcttat agatggtggt tttgctgatt tagtactgtc aggtacatca agtcactatt
    14341 gtacagcaga aagacaattt agattcccac ttgaacttgg aaatcaaaaa ccaatgacag
    14401 ctcagtggac agttacagga gcaggaagtg tattattagc tcctaatgga gacggtccaa
    14461 aagtaaagta tgttactgtt ggtaaagtaa tagatgaggg tattgatgat ggtaataata
    14521 tgggaccagc tatggctcca gcagcaatag atactatcta ctcttacttt gaagatacta
    14581 atgatgaccc aaatagcttt gatataattg ctactggaga tttaggtact cttggaaaac
    14641 agatagcaga agactttcta aaagaaaaag gtgtagatat atcaaaagtt tatactgact
    14701 gtggtataga gatgtttaac cttaaagaac aagatgtcca ctgtggtgga agtggatgtg
    14761 ggtgttcagc tacagtgttt gcaggatata tatatgataa gttgagaaaa aaagaattta
    14821 acaaagtaat gcttgtatca acaggagcac tactttcacc gacaagtaca ctacaaaagc
    14881 agacaatacc atgtgttgca catgcagtag taatagtaaa tgagtaggaa gtgatataat
    14941 atgttaatgg actatgtaag agtatttatt gtaggtggaa ttatatgttt aatagcccaa
    15001 gtaatgatgg attatttcaa aatgcaaact ccatatgtta tggtaacgta tgtaacaact
    15061 ggtgtagttt taacttttct aggactttat gagccattag ttaagtttgc agcagcaggt
    15121 gcaacagttc ctataatagg atttggatat tcacttgcta caggagtaat aaaatcaatt
    15181 caaagtgatg gttttttagg tattttttta ggaggtacaa ctgctacagc tggtggaata
    15241 gcagcagcta tattctttgg atatattatg tcagtaatat ttactccaaa agcaaaacca
    15301 taaatcttta ttaaaagaat aataagtttt tgaaataata actttttata aagcaggtgg
    15361 atatctttta gattccacct attttttata taaagaagcg tttacataat tgaagttgac
    15421 aattatttac aacatagtgt agtattatat tatcaaaata aaatgaagtg aagtagggga
    15481 taaagatggt aactattaaa gatattgcaa ggaatctagg tatcagttat tcaactgttt
    15541 ctaggtgctt aaacaataat cctaatgtat ctgaaaaaac aaaaaagaaa gtggtagaag
    15601 aggcaaatag actagggttc cattttaatg taaatgcaag aaatttagca aaaaaagaaa
    15661 ctaacaggat aggggtaatc ttttcaaata attttaatca tcaagatact agaaagtttt
    15721 ttagtgatat aatggatagt tctataaatt ctattgagac taataaatac gattttataa
    15781 tacagcctaa taataacatt agtggtgata gtaatgtata taaaatggta aatggacaaa
    15841 tggttgacgg attagtaatt gtgtctcaat caataaaaaa agaagagtat gattttttaa
    15901 aagacaataa ttttccacat gtatttatat attttaaacc atcttttata ggagaagtag
    15961 acaatttctt ttgggacgat aacgtgtatg gaggttatat agctaccaag catttaattg
    16021 accatggtca taaagatata ataacagtaa cttcagatga taagagttca aaaatgcatg
    16081 aagatagaac caagggatat ttgaatgcta tggaagaggc aaatttaaat accgaagtca
    16141 ttaaatctaa aatggatttt gaatctcaag ttgaattttt agaaaaatac attgataaaa
    16201 taaaaaaagc ttcagctata tttgtacaac aagatgtacc agcgatttct ataatacaag
    16261 agttaaaaac tacttatggt ataaatgtac cagaagatat ttccataata gggtataata
    16321 atattgaatt aatatcatat tttaggtcgc atttatcaac aatagatgac ccaagagagc
    16381 aagttataaa aaatggagta gatagtttag taaatataat aaataaaaag agtacagaac
    16441 acattccaag aaaattatat ccaaggttaa taatacgaaa tagcgttaaa agaataaagt
    16501 agtaagcttt taagtatcaa acgtttatta aaaatattat agattatgtg aagtttttta
    16561 acaaataata taaataaaaa ttaaaa
//
LOCUS       DAEMHW010000078        16470 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEMHW010000078 DAEMHW010000000
VERSION     DAEMHW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.078"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(60..377)
                     /locus_tag="KPK67_002094"
     CDS             complement(60..377)
                     /locus_tag="KPK67_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2459062.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(399..737)
                     /locus_tag="KPK67_002095"
     CDS             complement(399..737)
                     /locus_tag="KPK67_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2459063.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            1424..1990
                     /locus_tag="KPK67_002096"
     CDS             1424..1990
                     /locus_tag="KPK67_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBG2459064.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDN"
     gene            2295..2756
                     /locus_tag="KPK67_002097"
     CDS             2295..2756
                     /locus_tag="KPK67_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2459065.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            2824..5070
                     /locus_tag="KPK67_002098"
     CDS             2824..5070
                     /locus_tag="KPK67_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459066.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            5063..6895
                     /locus_tag="KPK67_002099"
     CDS             5063..6895
                     /locus_tag="KPK67_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459067.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            7614..8891
                     /gene="hflX"
                     /locus_tag="KPK67_002100"
     CDS             7614..8891
                     /gene="hflX"
                     /locus_tag="KPK67_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG2459068.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCLACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADILLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            9124..9621
                     /locus_tag="KPK67_002101"
     CDS             9124..9621
                     /locus_tag="KPK67_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2459069.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIEIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            9671..10195
                     /locus_tag="KPK67_002102"
     CDS             9671..10195
                     /locus_tag="KPK67_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG2459070.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILNYIREL"
     gene            10507..11871
                     /locus_tag="KPK67_002103"
     CDS             10507..11871
                     /locus_tag="KPK67_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBG2459071.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGVGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            12010..13176
                     /locus_tag="KPK67_002104"
     CDS             12010..13176
                     /locus_tag="KPK67_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG2459072.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            13293..14075
                     /locus_tag="KPK67_002105"
     CDS             13293..14075
                     /locus_tag="KPK67_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBG2459073.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYAESLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(14194..14847)
                     /locus_tag="KPK67_002106"
     CDS             complement(14194..14847)
                     /locus_tag="KPK67_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2459074.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(14871..16235)
                     /locus_tag="KPK67_002107"
     CDS             complement(14871..16235)
                     /locus_tag="KPK67_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG2459075.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         6346 a         1818 c         2687 g         5619 t
ORIGIN      
        1 aagacaaaaa aaggttattt atttcaaata accttttttt ttatataaaa tataaagaat
       61 tatattttat tcttttttat gatgtctata atctgtgata tcagatttag ttctcttgaa
      121 tttaagtctt ttaactcttc atttatatca ttttctaaat atgtctttcc agtggaagta
      181 atatctttta taagaacatc tgcagaaata tttaatatat ttacaaattt tatgaatgtt
      241 ttaagacttg gattcttttt accactttcg atttgagtta tataccaagg cgatactcca
      301 accatatttg caaattcttt tgaagttaaa tttgcttttt tccgttcttc tttaagtctt
      361 ttacctatat aattcattat tgttactatt tccctcactt atatttcttt gtatttaatg
      421 atgcacttta ttaactctag cattgcaatt tgttcttttt cagttaattt attaatcatt
      481 tcataacatt ctaaaggtac agagttatca acgccttctg ttgtaataat atttgcaaat
      541 aacaattcaa gtggtatgtc taaagcagta gcaattttca ttataatatt gatagatgta
      601 tttgtctcag ctctttcaat ctgaccaata tatgttggat gtaaacctgc cttttctgct
      661 agaaactctt gactatatcc caattttgtt ctatagactt ttattctatc tcctattatt
      721 tttcctatat ttgccaatta ttatccctcc actttagatt atattcatag tttaagttgt
      781 tttatataga ctatttatac ttttttagtc taaatataga ctatatatgt ttttatactg
      841 aaataagtgc tatttatttt tgtatagtct gtatctacaa taaacaaata tggataaatc
      901 atatttttaa ttagagaaat attttcataa tttcatagcc atactacaat aataaaacga
      961 attaataata tgttaattat agcatttttt attgtaatta catattagat atgtagaata
     1021 tattttgaaa attaaaaaaa ttttctattt tatatattga acaatttatg taaatagaaa
     1081 atttttcaat aagacttttg agtttaatca ttatattggt aaggtaaaca tttttaaatc
     1141 taattatata taggtgaaat aaaaaattaa ttataagtaa ggcatttaga ataaaatttt
     1201 aaaaaaatta taattaaaaa aataatgaaa atatataata ttagtttaga ttataaaaaa
     1261 taaatataca aatacttata aatacaagtt atatacaaga tttatagtag agtaataatc
     1321 ttataaaata atttaataaa tttaaattaa attatatgtg tatatattgt actttaatag
     1381 tgatataata ataaaaaaat ttttaaagag ggtgatatta catatgagaa aacttgaaga
     1441 gatattagaa tataataaat catttgtaga gaacaaagaa tatgaacaat atgtgacatc
     1501 aaaacatcca aataaaaaaa tagtagtctt atcttgtatg gatacaagat taactgagct
     1561 tttgcctaag gctatgaatt tgaaaaatgg agatgtgaaa ctaataaaaa atgcaggagc
     1621 tactataatg catccttttg gaagtattat gagaagtgtt ttagttgcaa tttacgaatt
     1681 tgatgtagat gaggttatgg ttgtagggca tcatggatgt ggtatgtgta atgttgatac
     1741 ggataagtta cttggtaaaa ttttagatag aggaatatct aatgatgtaa tacttacact
     1801 tcgtagtgct ggaattgata caaaacaatg gttacatgga ttcgattctg cagaagaatc
     1861 tgtaaaagat agtattgatt taataaaagg gcatccttta attccagatg gtattattgt
     1921 acatggtcta attatatcac ctgaaactgg gaagttagat gttgttgtaa atggatatga
     1981 agataactag agtatcataa tgttatataa tcaagatact gttgaataag atgttgtttc
     2041 aaaatttatt ttgggacaac ttttttattt gaaaatatat gaaaaggttt attttaagct
     2101 ttacaataaa aaatataagt agctgactaa ttaaaaatct tactattatt tattaaaatt
     2161 ataactatat gatataaaaa tatttaataa atgaaattta tatgaatttt ttataaatat
     2221 attgaaaaaa tgcatgcagt atagtaaaat aatgcttagc cgactaagta atataaaaaa
     2281 taaggagggc aagtatgaat agcaattctg aagaaaaatt aaatgatgtt ttcttgcaat
     2341 taatgcgaat atattttata agaactcatt tttattttga taaattaggc ttatatcatg
     2401 gtcaacccca aatacttttt tctttaaaga tgaaagatgg gcaaagtcaa aaagaattag
     2461 cagataaaag gaaagtaaaa gcatctacaa ctacagttat gataaagagg atggaaaaag
     2521 ctggacttgt ggaaagaaaa caagatgaaa aggaccaaag agtatctagg atttttttga
     2581 cagacaaagg tttagaaata tgtaataaag ttgaagaatt taacaaggaa ttggaaagag
     2641 agtttttttt aggatttagt gaagaagaaa aaatagtact tagaagactt ctaatgcaag
     2701 tacgagataa tttaaaagca ggatgtcata agcatgaaga tgaaaaatat ttttaaagtt
     2761 taaaataatg tttttcaatt taatgttatt agactaatta atattacaga agggaagaaa
     2821 tgtatgttta aattaattaa atatttaaag aaatcggcat tatcaatagt aataatagtc
     2881 tgtctattgg taattcaagc ggtatgtgac ctttctttac cagaatacac atccaatata
     2941 gtaaatgtag gtatacaaca aggtggagta gaaaactctg ttccatctgt gattagagag
     3001 agtgaattaa ataaaattac tttatttatg gataaaagta gtaaggataa agtactagat
     3061 aattatactt tgctgaataa aaaggattat gttaagtata aagataagta tccaggtcta
     3121 aaagatgaat ctctttatga gttaaatact aaagacaaag acactataga tgatttaaat
     3181 gttatttttg gtaaagctat tttaatagta tcagggcttg agggcgatag taaagaggta
     3241 aaagctatga aagctcagtt aatgagtaaa cttccaccac aagctactca aagtggagat
     3301 gttgatatat ttaaattgtt atcagcaatg ccaaaggaac agttagatac attgactaaa
     3361 gagatgagca aaggctttga aagtatgcca gagagtatga taacacaaag ctctgtttca
     3421 tatgtaaggt cagaatatga gaaaataggt attgatacag aaaaaactca aaataattat
     3481 atattattta ctggtgctaa aatgcttggt atagcaatga ttagtatggt agctacaata
     3541 acagtaggat ttttagcagc aagagtagca gcaagtgtag gtagaaattt aagaagtgga
     3601 gtatttagaa aagttatgtc attttcaaat actgaaatga atgaattttc tacagcttca
     3661 ttaattactc gtagtacaaa tgatattcaa cagatacaaa tgttgatggt tatgttgctt
     3721 agaatagtat tctatgcacc tataatggct ataggtggag tgataaaggt tttaaacaca
     3781 aatacttcaa tggcctggat tatagcagta gcagtagttg ctatcttatc attaattgta
     3841 gtgttattct cagtggttat gcctaagttt aaactggttc aaaagcttgt tgataagtta
     3901 aacttagtaa gccgtgaaat aattactggt attccagtta tcagagcttt tagtactgaa
     3961 aaacatgaag aagaaagatt tgataaagca aataaaaatt taactaaaac aaatcttttt
     4021 gtaaatagag ttatgacatg tatgatgcct gctatgatgc ttataatgaa ccttatcaca
     4081 gttcttatag tttggagagg tgcatacagt gttgattctg gagcaatgca agttggagat
     4141 atgatggcat ttatacaata tacaatgcaa ataataatgt cattcttaat gatatcaatg
     4201 gtatctatta tgcttccaag agcatccgtt tcagctacac gtatagatga agtattagtg
     4261 tcagatataa gcataaaaga cccagaaaaa tcagaggcac aagagtttaa ggaagataaa
     4321 aagggtcttg ttgaatttaa aaatgtttcg ttcatgtatc cagacgcaga agaacctata
     4381 ttaacaaata tatcttttac agcaaagcca ggagaaacaa ctgcctttat aggaagtact
     4441 ggtagtggta agtcaactct tataaattta attcctcgtt tctttgatgt tactgaagga
     4501 gaaatattac ttggtggagt taatataaaa aatgtttctc aacatgattt aagggaaaaa
     4561 attggatatg tacctcaaaa gggagtattg ttttctggta ctatagaatc caacttaaaa
     4621 tatggtagaa aagacgcaac agatgaagaa atgagaacag cagctgaaat agctcaagct
     4681 acagaattta tagattctaa accggaagct tttaaaactg aaatttctca aggtggaaca
     4741 aatgtatctg gaggtcaaaa acaaagactt tctattgcaa gagcaatagc aaaaaatcca
     4801 gaagtttata tatttgatga tagtttttca gcacttgact taaaaactga tgcagctctt
     4861 cgtaaggctt taaaatcaaa aactgctgaa agtacagttc taatagttgc acaaagaatt
     4921 agtactatct taaatgcaga acagataatt gttttaaatg agggagaaat agttggaata
     4981 ggaacacata aagagcttct aaaaaattgc gaagtttatg agcaaatagc tttatcacaa
     5041 ctttcaaagg aggagctagc taatgagtag aatgggaaga ggtcctatgg gaaaatctat
     5101 gggagcagga caaaaagcaa atgactttaa agggactatg cgaaaactaa ttgcatattt
     5161 atctaaattt aaaatatcaa taattctagt aattgtattt gctattggta gtgcgtcgtt
     5221 ttctatagta ggtccaaaaa ttttaggaaa ggcaactaca aagatatttg aagggctagt
     5281 gagtaaagtt tctggaggaa acgtaggtat agattttaat gcaattggaa aaatcttaac
     5341 ctttttactt ttcctttatt tgataagtgc actgttctct tttatacaag gatttataat
     5401 gagtggaatt tctcaaaaag tatcctataa tttaagaaaa gagatttcag caaaattgga
     5461 tagattgcca atgaagtatt ttgatactaa gacgcatggt gaaatattat cgagaattac
     5521 gaatgatata gatactttaa atcaaagttt aaatcaaagt atgacacagc ttataacatc
     5581 agtaacaact atgataggtg ttttgataat gatgttatct ataagtggaa ttatgacatt
     5641 agtggcagtt ttaatattac caatatcaat gtttgtaatt tcaagaataa taaagaaatc
     5701 acaaaaatac tttagatatc aacaagaata tttaggaaat gtaaatggtc aagttgagga
     5761 aacttacagt ggccaaacaa tagtaaaagc atttaatcgt gaggatgaag taattgaaga
     5821 gtttgataaa ctaaatgata gcctgtataa ttcagcgtgg aaatctcaat ttttatcagg
     5881 tataatgcaa ccacttatga tgtttattgg aaacttggga tatgtaatgg tatcaatctt
     5941 aggaggttgg cttgcaatta agaaaacaat tgaggttggt gacatacagt catttataca
     6001 atatgtaaga aactttacac agccaatgac tcagattgca caggttgcca atttattgca
     6061 atcaacagca gctgcttctg aaagagtatt tgagttttta gaagaggaag aagaagtcca
     6121 aattgttgaa aatgcagttt caatagatgg tttagaaggt aaaattgatt ttgaaaatgt
     6181 caactttgga tataacccaa acaagactat aataaatgat ttctcagtaa atgttaaacc
     6241 tggtcaaaaa gttgctattg ttggacctac aggagcaggt aaaactacaa tagttaaact
     6301 gttaatgaga ttttatgatg ttaatagcgg ttcaatactg attgatggtc ataatattaa
     6361 agattttaat cgtagtgagc ttagagaaat gtttggtatg gttcttcaag atacatggtt
     6421 atttagtggt tcaattatgg aaaatataag atatggtaag ttgaatgcta cagatgaaga
     6481 agttatagag gctgctaaat ctgcacatgt tcatagattt attaagacat taccagatgg
     6541 atataagatg aaattaaatg aagaagcaag taatgtttct caaggtcaaa aacaattatt
     6601 gactatagct cgtgctattt tagctgatcc taaaatttta atattagatg aagctacaag
     6661 ttctgtcgat actagaacag aagtattgat tcaaaaagct atggataatc ttatggaagg
     6721 tagaacaagt tttgttatag cccacagatt atcaactata cgtgatgctg atatgattct
     6781 tgtaatgaat gaaggagata tagttgaaca aggaaatcat gaggaacttc taaagaaagg
     6841 cggattctat gccaatcttt acaattctca atttgaagaa gatgaagcaa tgtaacaaaa
     6901 aactatctta gcatagacat gtaattaata agataaaata aatgagggtg ccttaaaatg
     6961 aactttttaa ttcataaggc actcttttgt gtatgaaata aagtgtattt ttatcatttt
     7021 aacaatgaaa aagaggattc tctatatttt gagatagcca cataatttta tgtctttaca
     7081 aagtgtttat tttattataa tatgtagtaa ggaaaatgtt ggaggtgatt atcaaaatgt
     7141 atatttaatt ttattcttta aattttatta taaatagaat ttaattttaa tagttgatga
     7201 ttatattgaa gtatatgttt ttattacttt tattaatcat gcaaataact acttaaattt
     7261 aagaataggg ttaaatataa aatgataatt acatataagt taatcaaact aatgtatttt
     7321 taatatatat ttagaatttt aatccttctt atgatataag tagcttgttt tgtattactt
     7381 agatttgtaa ggagtttttt tattgaaaaa actgcataat atatttggga ggaggtgtaa
     7441 gaaagtgatt ttttatataa gattgagaaa ataatgcacg attatggtta ttataatagt
     7501 aaataagaag tgtcatttaa ggataagata aaaagcaaaa ttcttggtga atttaattat
     7561 agagccataa tctcgtgcta tagattaaat aaaaaatagc agataaggag aatatggata
     7621 tggcgaaaaa aggaataaca gtaggattaa acataaataa taaatctgag gactttaatg
     7681 aactaatgat tgaacttgaa aacttatgtt tagcatgtga tattgatgtt gtagggagta
     7741 taactcaaaa tgcaaagcag gttaatagag cattttacat tgggacaggt aaagttgagg
     7801 aaatattaaa cttaataaag aaagaaaaca tagagatagt tatattttac aatgaacttt
     7861 ctacatcaca actaaaaaat cttgaagaaa agcttaattg tgaaattata gatagaacgg
     7921 cattgatact tgatatattt gcacaaaggg caaaaactag agaagctaaa cttcaagtag
     7981 aagtagcgag tttaaaatat atgttaccaa gacttatagg ttcaaatgaa aatttaggac
     8041 gtcaaagtgg tggagttggt actaaaaata gaggttctgg tgagaaaaaa ttagaacttg
     8101 accgtagaag aatagaagaa aagataacat ctttaaatag ggagttggat gatttaaaat
     8161 tccaaagaga aactcaaaga agtatgagaa gaaaatcaaa tcttccaaat gtagctctag
     8221 ttgggtatac aaatgctggt aaatcttcaa taatgaataa actagtagat atatttaaaa
     8281 actctgaaga aaagaaagtg tttgagaaaa acatgttatt tgctacactt gaaacttctg
     8341 tcagaaatat agttcttgca aataacaaag aatttttatt atctgatact gttggttttg
     8401 taagtaatct accacatgac ttagtaaaag catttaggtc aacattagaa gaagcatgtg
     8461 aggcagacat actgttacat gttattgata tttcaaatcc aagctataaa agtcatatta
     8521 aagtgacaga agatacttta aaacaaatag gtgcagatgg tatacctatg atacatgtgt
     8581 ataataaaat tgatttaata gatgtagaag tattggatag aattttagat agtatcgata
     8641 aagaaggtat atttgtttca gttaaaaaag atataaatat agataaaatg ataaaatgta
     8701 tttgtgatag catatttaaa gattatgtaa gatgtaagtt tttgattcca tatgataaag
     8761 gtcatgtagt ttcttatttt aacgaaaata cgagcattat aaatacagaa tacagagaag
     8821 atggagctat tttagatgta gaatgtagca atatagaata taataagtat aaaaagtatg
     8881 ctttagagta aattttgaaa aacttattat agcctaaaat atatttaatt tataatttat
     8941 tataaattga ttgtaaatat gattttatga aacggtatag ccataatgct atgccgtttt
     9001 gccatttata ataaaaataa atttattgac ataatagtaa ctaattacta taatataaag
     9061 taattagtta ctatttaaaa ttatcaaaaa attaaaatct aagataaaga gaagtgtata
     9121 ataatggatg cagaaagttt aatgaaaaaa tatatggtag atgatgatag tagaaaacaa
     9181 gctatatttg caagtatatt tgtaatgcaa aataaacttc aaactacatg tgataaatta
     9241 gacccagaat taacaatgaa acaatggtta cttttagcta ttgcggactc tgtagatgaa
     9301 actcttacat taaccaaact tggagaatta atggggtgtt caagacaaaa tgtaaaaaaa
     9361 ctagctattg ctttagaaaa gaaaggtttt atagaaattg agcaaaatga aaaagattca
     9421 agagctatct gtcttgtgac aaaagataga gtgcaagagt attctaaaaa agtaagaggt
     9481 atgcaggaaa aagtattaca gcttttattt gaagatttta cagagaaaga agtggaacag
     9541 ttattttttg gaattatgaa attaagtata ggaattgaaa atgtgaaaaa ttatgtagaa
     9601 aataatgacg ttggagaata agcaagtatc aaatcaactt tgtatgacat cagagagggg
     9661 ggtaatttat atgaaaaatg tagtagtgat ttataagagt aagtatggtt caacaaaaaa
     9721 atatgctaaa tggctttcag aattattgtc ttgtgatatt tttgaaacta agaatattaa
     9781 tatagaaaag gttatgaagt atgatacgat tattttagga ggaggtcttt atgcttgtaa
     9841 tatagcggga atttctttct taaaaaaaaa ttataaagaa ttgcagaaaa aaaatttagt
     9901 agtatttggc gtaggagctg ctccatatga tgatgaagta ataaagaaat taagagaagt
     9961 aaattttaag gatgaattaa acaaaattcc ttgcttttat tgtcgtgggg catgggatga
    10021 gacaaaaatg tcttttatag attatactct ttgtagtttt tttaaaaaat ctatagctaa
    10081 aaaagaacgt caaggtctta aaccagcaga agcaccttta ttgtctggag aagcatttga
    10141 ttggtgtaat aaagaaaatt taactccaat acttaattat ataagagaac tttaagaggt
    10201 atatatttat aattatttat tatctaaagt tagatttcat agaaacttac tagacattat
    10261 ttataaaata atattattaa agatggataa tttaattgat tcaataaggc tataatttta
    10321 aaaaatgttt attttaaaat aaattatttt ataagcttac ttatacttaa taaggggctt
    10381 tttttattgg aagatttatg ttatattgag atgaatttat tatgatataa aaaataatgt
    10441 taggataaat ttaaatcttt ttctaaaaat gtattaatat aattcttaaa gaaaggattt
    10501 ttaataatgg atataaaaga gttagttaaa atgcaaagaa aatattataa tacaggtaaa
    10561 acaagagata tatcgtttcg tatagaacag ttgaagaaac ttaagttagt tgtatcacaa
    10621 aatgaagaaa aaatactttt agcactaaag aaagatttaa ataaatcgga ttttgaaggt
    10681 tttatgacag aagtaggcat gttttatagt gaattaaact ttgctataaa gaatataaga
    10741 aaatggagca aaataaaaag agttaaatca agtatggtta attttccatc tatatccaaa
    10801 gttgttccac aaccttatgg tgttactctc ataatgtcac cttggaatta tccatttcaa
    10861 ttagcactta tcccattagt ttggtcatta gcagcaggga attgtgttat attaaaacct
    10921 tctgaatatt ctgtgtcaac atctagtgta gttaaagaca tagttgaaga tactttttca
    10981 aaagaatatg ttgcagttgt tcaaggaagt caagaagaaa gtgaaaagct actattggaa
    11041 agatttgatt atatattttt tacaggaagt actaatgttg gtaaaataat catgaaatca
    11101 gcatcagagc atttaactcc tattacttta gaacttggag gaaaaagtcc ttgtatcatt
    11161 ttaaaagatg ctaatataga ccttactgcc aaaagactta cttggggaaa actaataaat
    11221 gcaggacaaa cctgcgtggc tccagattat gttttagtgc atgaagatag aaaaaatgag
    11281 ttaatagaga aaataaaata ttatactaat aagtattttg gagataatcc atgtaataat
    11341 gagcaatttc ctaaaatcat aaatcagaga cattttaatc gaatattatc tcttattgat
    11401 aaagataaga tagtctatgg tggaaattat aataaggaga cattaaagat agagccaact
    11461 atagtagaca atgttaattg ggatgataat ataatgaagg aagaaatatt tgggcctata
    11521 tttcccattt taacatataa agatttggat gaagtaatac aaaaaattat acaaatgcca
    11581 aatccattgg cactttatat ttttacaaaa aataagtacc tagaaaataa acttttagaa
    11641 atgattccag caggcggatg ttgtatcaat gatacagtta ctcatatagc tacgaattac
    11701 ttaccttttg gtggtgttgg tgaaagtggt atgggctcat atcatggaaa agctggattc
    11761 gatacattta ctcactataa gagtgtgtta aaaaaattaa atttggatgt ccctattaga
    11821 tatgctccat acgataataa attaattaag gttcttaaaa agataatgta ggttatattg
    11881 tagaataata aatataaata aaattgagta tttgtattct gaatatattt tatttttaat
    11941 ttggtatatt tgttataaaa gtggtgattt tgtgataaaa tttaattata tagaaataag
    12001 gaggaaaaaa tgaaaattaa ttggaataca aaatacacaa caatagcaat ttatacattt
    12061 ataatagctg ctagtagtat tatattttat ctagtatcat cacaaattga tgtattttct
    12121 aataatttag atgctatatt tacaacatta caacctttta taataggatt tgctattgca
    12181 tatcttctta attttatttt aaagttttat gaagacagaa tttttataaa aagtgagaaa
    12241 ttaaaaaaac taaaacaaag ttcaaaaaga ggcttgggtc ttttattaac atatgcaaca
    12301 gctacattga tactttactt atttatgcat ttcgtattgc cacaagtaat tgagagtatt
    12361 gttggactag caaatgatat acctatgtat gtaaacaatg caactaagct tattgataaa
    12421 ttaatgactg atttaaatct tgatgagcaa tactttaatt tggcagttga taagtggaat
    12481 gagtttgtta catatattat aaagtttgta acagatttaa tcccaatatt aggaagtatg
    12541 ttaaagaatg tagcctctag tatatggaat attgtactag gattaattgt ttcagtatac
    12601 ttattaattg ataaagaaaa attctatgga ttaagcaaaa aaataactta tgcagtattt
    12661 acagaaaaac aagctgccag aattttagaa ttaactcata gaagtaacta tacatttgga
    12721 agatttttag gtggcaaaat attagattca ttcattatag gtattctaac atttgtaata
    12781 cttacattag ttaaaatgcc atatacatta ttaatatcag ttataattgg tattacaaat
    12841 attatccctt tctttggacc tttatttgga gctataccat ctacgctaat agtgatgttt
    12901 gtatctccta taaaagcttt ctggttattg ttgattatat taataataca acagatagat
    12961 ggaaatatta taggacctaa aatacttgga gattcaattg gtatatctgc attttggatt
    13021 ttgttctcct tattggttgc aggaaaatta ttaggtttta ttggaatggt tataggagtt
    13081 ccaatgtttg cagtaattta ttctataatt aaggatattg ttgaaagtaa attggacaaa
    13141 aagggattac cgacagatac ttccgattat atgtagctaa aaaatattat gtataattaa
    13201 aaatatataa attaagcaat atttaaagca ctgagaaagt taaactcttg gtgcttttta
    13261 gatataaaat tattatagaa gaggtgtttt ttgtggcatt tatagaatgt aaatttaaat
    13321 ctaaatatac tggaggagaa actgatataa ttgtaatttt gcctgtagca aatggaaaag
    13381 aactttttag tggtaaagag atatatgatt ttgaaggtaa atttaaaaca ttatacttat
    13441 ttcatggttt agaagatgac caaagctcat ggattagata tactaatgta gagcattatg
    13501 ctaaaaataa aaatatagcc ctagtaatac caagagtaga aactagtttt tataccgata
    13561 tggcgtatgg acaccaatat tttacttttg taagcgagga attacctaaa tttgttagag
    13621 ctatatttcc tttatcagat aaaagggaag ataactttgt agctggtatg tctatggggg
    13681 gatatggagc tttaaaatta gcgctttcaa aaccaaaaga gttttcggca gttgcaagtt
    13741 tttctggttc gcctgatatg attcatgagt tagaaaatca acaaatagaa accaatgcag
    13801 acattctatt tcacgatttt ggtagaccag aagacgctag aaatagtgag aatgatttac
    13861 tttacatact taagaacctt aaagaaagaa atgaagatat tcctaaaatt tatcaatttt
    13921 gtggtgatag agatttttta tataaaaata atcagatgtt taaaaaatat gcagagtctc
    13981 tgggtgtaga tgttgaatat gaagagtgtg taggggggca tgaatggcgt ctttggaatt
    14041 tatggataaa gaaatttata gatataattg ggtagtacac ttatggtatt aacaggtata
    14101 ctataaaata tctttaaata tgataaagac tgaaatccta aaatcaactt atgattttaa
    14161 aatttcagtc tctaagaatt taataaaatt ttattaagtt cttacacgag ttattattgg
    14221 agtaaaattg tcaactatat ctatatagta tttactcaat tcctctaaaa tctcttcgca
    14281 taaatccgta ctatgaccaa gattaataag aaactgtttt gtaaaatgct ttattacagc
    14341 ttgagaagat atatcgtact tgtgtgaagt ataaactcta attatttcag gtttcatagc
    14401 aagtatacca atacaagaaa gtattaattc atcatgttct atagattctt tatcattaac
    14461 atatcttttc atgaccattt cggttacact tctttgatag cttaaaatag cttcttcata
    14521 agctatatcc aaataaaaat ttctatatgc ttcgtttttt agaaggaaac aaaggtcagc
    14581 attcatagca agaaagaaca tcatcatagt accttcttca tggtagttat agattaaaga
    14641 ctgcatttca tttcttatat cttcatatat tatagctcct atttcgcttt tacttgagaa
    14701 gtaatagctt attagaccta aatttatatt agctctttct gcaatttgcc tagttgttgt
    14761 cttatgataa ccttgctcaa aaaataattc tctagcagca cttataatac tagcttttgt
    14821 ttctttgccc ttttctgatt ttgacatatt tactgtgtat acctctcttt tcaatctttt
    14881 tttgaaaatt tgctaactat aaatattgta atagaaccac ctattatagg aaccaaaatt
    14941 ctgtcaaata cagtaagaat accaatcata tttaataatg ccaatataac acttactata
    15001 acagcataga tagctccttt ttttgtagca cctttccagc aaagaccaat tacaaatggt
    15061 gcaaatatta cacctgtaca taatggagct ggccaaaaca ataattctaa taaactatca
    15121 aatttaagtg ctatgattgc acctattata gctgtcaaag ctgatgcaat ttgagaatac
    15181 tttagagtat tttttaaatc attaaattct atattaggat gtaataattt gttataaata
    15241 tctcttgata taatagcact cattgaggtc attaatgaat cggcagtaga cattatggca
    15301 gccaatatag cagctaagaa taatacagat attaaaggtg gcaaagtagt cgatatcatt
    15361 tgaggtaaaa cctgaacaga tgatatgttt ggaaataaac taccaccaat agctccaatt
    15421 attcctatag ggaaagcaag aaataatagt ataaatcctg caattacact acttttaact
    15481 gcatttttag cattatcagc acattgatat cttaaaaaac tttcataaga aaatattgag
    15541 cctaaaacta atggtaaaac tgtatacata aaatcactaa tcgatttatt tcctggaaag
    15601 aatggttctt ttaccattga ttgtaacata agtaaagggt tgtcgccaac taattttaag
    15661 cttatataag ctactattgg caaaaacact atacaaatag caagttgaat aaatgctgat
    15721 actgcaactc cccacattcc tgatgtataa gtatatataa tgataactat aactcctata
    15781 attacagcta tatcataagg aataccaaat gcagtagata cagaaccaaa agaaatcaat
    15841 tgactaccca taatagctag tatagatatt ggtgttacaa gactttgata agctcttaaa
    15901 aatacactat gaccatatct agtttcaata taatcagcag gtgttatatc acaagataag
    15961 cttcttaatt ttcctgcgaa aaatgcattt ataaagcaag ctaaagctgc acctatagta
    16021 gaatacattc caacccaaag tcctctttca gctccccatt gaactccacc aactaagcac
    16081 cctcctccaa aagatgtagc agcaatggtt gcagctagta taggcaaagg cagatttcta
    16141 cctgcgatta ggaaatcgga tgctgtttta actttttttg aaaaataaaa accaatccat
    16201 atgtatattg cacacgtaac aattaaaagt aacatagtct tttctccttt ataaaatatt
    16261 ttaataaaat atacaaaata tatttaaaat atattttata taaaagtagt acgatatttg
    16321 ttttattggt tttagattaa tatagctata ataatttatg atattagatg gaaattacgt
    16381 aaaaatagaa aattgaaaat tctgaaattt ttgcatattg atatttatct catagattct
    16441 tttttgtaat tgtaacacgc ttttattttt
//
LOCUS       DAEMHW010000079        16371 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEMHW010000079 DAEMHW010000000
VERSION     DAEMHW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.079"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(203..1561)
                     /gene="rlmD"
                     /locus_tag="KPK67_002108"
     CDS             complement(203..1561)
                     /gene="rlmD"
                     /locus_tag="KPK67_002108"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG2459076.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSENLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(1736..3496)
                     /gene="pyk"
                     /locus_tag="KPK67_002109"
     CDS             complement(1736..3496)
                     /gene="pyk"
                     /locus_tag="KPK67_002109"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBG2459077.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(3548..4507)
                     /gene="pfkA"
                     /locus_tag="KPK67_002110"
     CDS             complement(3548..4507)
                     /gene="pfkA"
                     /locus_tag="KPK67_002110"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG2459078.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(4716..8285)
                     /locus_tag="KPK67_002111"
     CDS             complement(4716..8285)
                     /locus_tag="KPK67_002111"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBG2459079.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMNSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKRHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(8442..9389)
                     /gene="whiA"
                     /locus_tag="KPK67_002112"
     CDS             complement(8442..9389)
                     /gene="whiA"
                     /locus_tag="KPK67_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBG2459080.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(9406..9831)
                     /locus_tag="KPK67_002113"
     CDS             complement(9406..9831)
                     /locus_tag="KPK67_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG2459081.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(9900..11006)
                     /locus_tag="KPK67_002114"
     CDS             complement(9900..11006)
                     /locus_tag="KPK67_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBG2459082.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(11018..11875)
                     /gene="rapZ"
                     /locus_tag="KPK67_002115"
     CDS             complement(11018..11875)
                     /gene="rapZ"
                     /locus_tag="KPK67_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBG2459083.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(11906..12619)
                     /locus_tag="KPK67_002116"
     CDS             complement(11906..12619)
                     /locus_tag="KPK67_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG2459084.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYNDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(12623..13537)
                     /gene="murB"
                     /locus_tag="KPK67_002117"
     CDS             complement(12623..13537)
                     /gene="murB"
                     /locus_tag="KPK67_002117"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBG2459085.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(13810..14580)
                     /locus_tag="KPK67_002118"
     CDS             complement(13810..14580)
                     /locus_tag="KPK67_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG2459086.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAMAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            14898..>16371
                     /gene="cls"
                     /locus_tag="KPK67_002119"
     CDS             14898..>16371
                     /gene="cls"
                     /locus_tag="KPK67_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG2459087.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSA"
BASE COUNT         5004 a         3113 c         1830 g         6424 t
ORIGIN      
        1 aaatgagttg tataaaatct ggctactgta gattgacgat ttcttttaaa attacaatta
       61 taataagttt tatagaatta aaaataaaac tttagagatt tttgataaca ttgtagacta
      121 aatttgccac caaaatcgct ttacagtggc aaatcggtgg caaatacaat atattattag
      181 tatataatct atataaacat tactattgtt tgattatctt agctacagtt tccacatgtg
      241 tagaatgagg gaacatatct acaggttgtg cctcaacaca tttaaatccg tgaacattta
      301 gataatctaa atcccttgct aaagtagaag gattacaaga aacatagaca attctttccg
      361 gattcattga aacaatagtc tctaatacct tttcgtcaca tccctttcta ggtgggtcta
      421 caactactac attggcagtc ttaccctctc tatacatctt aggtacaatt tcctctgcct
      481 ttccaacata aaattcagca ttatgtatat tgtttaattt agcatttatt ttagcatctt
      541 ttatggcatc atctataatt tctattccat aaaccttttt agccttttgt gccaaaaata
      601 aagatattgt acctatccca cagtaaatat caaaaacagt atcagtatca tttaaatttg
      661 catattcaag agccttatca tacaatactt tagtttggac agggtttact tggaaaaatg
      721 ataatggtga aatctcaaac actaagtcac ctatataatc gtttattttt ccatctccat
      781 atataactat attttctctt cctagaatag cattcgtatc tttttgattt acatttacaa
      841 ccaaagtctt aaatccaggt atattttcct taagcactga agctaattct ttaagatatg
      901 gaagtttgtt tccatttgct actaatacaa ccattacttc tttagttgta aaacctattt
      961 ttgttactaa atgcctaatc aaccccgtgt gagtcttttc atcatatata cttaccttat
     1021 aggcccttat atatgttttt attattttaa caattttatc atttatctca tgttgtatta
     1081 cgcatttttc tgtcgatatt acatcatgag tcttcttttt atagaagcca ataattggaa
     1141 taccattatt cttttgtatt ggaaattgtg ctttatttct atatctatat ggattatcca
     1201 ttcctatagt gtcatgaact tctacatcct ctaacttacc tattctcgaa agtacttgtt
     1261 taacttcatt tgttttcatt tcaagttgtt tattataatc taagtcttgt atctgacacc
     1321 ctccacaatc cctaagattc tcactacaca ctcttttcac cctgaatgga gacttttcta
     1381 ttatctcaac aatatctccc acagcataat ttttctttac tttattaatc ctaactttaa
     1441 ctttatcatg tattaatccg ccctctacaa aaactgtaat accattgtac tttcctactc
     1501 ctactccgcc ttgaccaatg tctactatat ctacaatata catctcgttt ttcgaaagca
     1561 taaatcaccc tccaatcaat ctattatatt taaatcttta caaacttttt tctttaaatt
     1621 tatattaaaa tattgctatt aattaaacta tattttttac taagctacaa tattttattt
     1681 taccaaaaaa aaaggagctt ataaagcccc ttagtaaata aatttattat ttttcttata
     1741 aaactctagt tgaacctcta tataccacac ctcttgatgc atccacagtt acaacttctc
     1801 catcttttac tagatttgtt atatcagttg cagacacaat aactggtata tctaaattta
     1861 tacctacaat agctgtatgt gatgtcattc caccattctc agttactaca gctcctgctt
     1921 tttctatata attgttcatt tcagcatcag tagcagtaga aacaagtatg tcgccttcat
     1981 taaagtctac acaagcattt ccattcttta ttatacgaac tttaccttct actgtttgag
     2041 tacctacacc tataccttgc acgatttctt cgcttattac atgaaccttt attaaattag
     2101 tagttccact tactcctact ggtactccag cagttataac tactaattcc ccattatcta
     2161 tataatcagc ttgtctagct gcctcaattg ccttttctat aacttcatct gtatttcctg
     2221 caacagaaga cttaactgga tatactcccc atgtaagagc taatttattc atagtctttt
     2281 cattatttgt agtagctatt attggagatt ttggtctgaa tttagatacc attctagcag
     2341 tatatcctga agaagtagat gttattatag ctgatgcatt taaatctact gctgttgtac
     2401 aagttgcatg gcttattgca tctgttacag taacattgtt agtaccattt tcctttaata
     2461 atctatcata atctaaagtc tcttctgttc tttttgctat tgtagccatc atttttactg
     2521 cctctacagg atattttcca gcagcagttt ctccagataa cattatcgca tctgttccat
     2581 catatatagc attggcaacg tctgtaactt ctgctcttgt tggtcttgga tttcttatca
     2641 tagaatcaag catttgagtg gcagtaacaa ctggctttgc taattcatta cattttttta
     2701 tcatcatttt ttgaactatt ggcatctctt cagttggtat ctcaacacct aagtcacctc
     2761 tagccaccat tataccatca gaaactttta atatttcatc taggttctct acaccttctt
     2821 ggttttctat tttagatatt atttgtatat cagttgcatt attgttctct aatacttctc
     2881 ttattgctag tacatcagat gcctttctta caaaagaagc agctatataa tctattcctt
     2941 gacttattcc aaactctata tcacttatat ctttaggagt tattgctggt aaatttatct
     3001 taacaccagg tacatttact cctttatgat ttttaactat tcctgaattt tcaacaacac
     3061 aaactatatc ttctccattt atttctttta ctcttaagcc aactaaacca tcatctatta
     3121 gtatagaatc tcctgattta acgtcttcag caagaccttt atagcttact gtacacattt
     3181 ctttagtccc cataatatct ttcatagtta tagtgaactt ttgcccttct tctaaaaata
     3241 cctctgggtc ttcaaagtta cctgttctaa tttctggacc tttagtatct aataatatag
     3301 caactggctt atttaatttt tctctaactt tctttgccat atcaattctt tctttatgtt
     3361 cttcatgcga accgtgagaa aagttaaatc tacacacatt tagaccattt tgcataagtt
     3421 gagttaaaac ttcctcactt tgtgatgctg gacctagtgt acaaacaatt tttgtcttct
     3481 ttacattatt taacatattt gctcctcctg agatattatt ccacagattt atttttaata
     3541 ctaaaactta tatagataat atttttgcca tctcataagc ttctttatca aatactttct
     3601 tttgagctaa agcttcactt atttctaaat caattatttt gttttctttt attcctacaa
     3661 ctctagctga ttttccatct aataataatt ctactgctct tactccaagt ctacttgcta
     3721 gtattctatc agatacagtt gggcttcctc ctctttggac atgccccaat acagtgactc
     3781 ttaaatctgc tcctgactct tttttaagtt ccttttctaa atcactagca cttccgactc
     3841 cttctgctaa aacgattata ctatgtctct tacctctttt ttgagtagtc tttaatctta
     3901 gagcaacttc atctactgta atttctactt ctggtactat tatagtttcc gctcctcctg
     3961 caagacctgc atataaggct aaatctccac agtgtcttcc cataacttct actatgttaa
     4021 ctctttcatg agatgatgat gtatctctta tcttacctat agcatctata atagtattca
     4081 ttgcagtatc aaatcctata gtataatctg tatatgctaa atcgttatct atagttcctg
     4141 gtatgcctat agctgggaat cctaagtcac taagtttttg agctcctgca aatgaaccat
     4201 ctccaccaat tacaactaag caatcaattt tatacttttt aagaattttt actgctttta
     4261 atctaccttc ttctgtttta aattcttcac atctggaaga ttttaatata gttcctcccc
     4321 tgtgtataat atctccaaca gaagataagt tcatttcagt taaatcttct tctagtagtc
     4381 ccttatatcc tctattgatt ccatatactt tgcatccata atatattgct gatcttacaa
     4441 ccgctctaat cgcagcattc attcctggag catctcctcc acttgttagt aatcctatag
     4501 ttttcatttt ttacctcctt ggattttatg tcaatttttt attataatca ttgttattgt
     4561 attctaatgg actttttttg tccctatatc actttttaat cccatcaaga ctaaaaaaat
     4621 taaatgtcct taaatgtcca ttttgtgata ttatatcaca aaacggacaa tatttttcat
     4681 attattataa tatatttaga tttttatata aaaaatcaag cttttacttt tatgttatct
     4741 ttaggaagta actcttctaa gttatttata aattcatcac atatacaagt caatgccccg
     4801 gtcattttat atgctttacg tttaccattt atattttcat ccatagggta caagtatata
     4861 ctgtcattac ctggatattg tttggctatt tctacaatat tattagttgt atcagtatca
     4921 gacatactat ctattttcaa atacaatttt ttaccacttt ctggcttaat attttctaca
     4981 tttctagttc ttcctgaatt gtagtttcta tctgcaaagt cagtatcatc atttatatct
     5041 tttatctctc tagctataag ttttgcattt tcgccttctt ttatacttaa tgttcctttt
     5101 acatatatta tattatcttc attcaaaatg acattatact tttgtaataa ttgaggaaaa
     5161 actattattt ctatagcacc atacaaatct tctaattcaa taaatgccat aatttcattt
     5221 cgttttgttg ttttaatggt tttagttaca atcattccac ccattataac atctgtttca
     5281 ttcatttgta ggtatgactc ttcttcctct tttaatgaat ttaattttcc attatccata
     5341 gatgtttttt cttgtagttc tttttcaaat tgtgataaag gatgtccact tacatacatt
     5401 cccaagactt ctttttcaag gttaagtctc tctctttctt catactcttg ccttttaggt
     5461 aaactgtata tttgttgaaa atcactatct tcctcactat ctccaaaagc atcaaataaa
     5521 gaaatttgac cttgaacatt tttctttcta tccatagata cactttctaa taaactttca
     5581 taaccagcca tcaaactagc tctattttca cttatattat caaaagctcc acattttatt
     5641 agactttcta ttactctctt atttgtatct ttttggtcta atcttttgac taaatctact
     5701 aaatctacaa ataatccatt agattctctt tcagcgatta tattgtttat tatatttacg
     5761 ccaacatttt ttacggctgc tagaccaaat cttatattgt cgccttcaac tgaaaacttt
     5821 gaaaaacttt tgtttatatc tggaggtaat acatctattt ctagtgcttt acattctctt
     5881 atatactcaa ctaccttatc agtatttccc attacactgg ttatcaatgc tgccataaat
     5941 tcaactggat aatatgtttt tagataagct gtctcatatg ctaaaacccc atatgcagct
     6001 gcatgtgatt tattaaatgc atatctagcg aagtctatca tatcatcaaa tattttatta
     6061 gctatttcct cttctacacc atttctgata caccctgcaa tttctatatt accatcttca
     6121 tcaaatttac catgaataaa atattgtctt tcttcttcca ttacgtccat ctttttctta
     6181 ctcatggctc ttctaactag gtcacttcgt ccgtaactat aaccacctaa atctctaacg
     6241 acctgcataa cctgctcttg atataccaaa caaccatatg taacttccat tataggcttt
     6301 aatttttcat gtagatatgt tacatctttt gggttgcttt tattttttat atatgcaggt
     6361 atagaatcca ttggacctgg tctaaatagg gatattcccg ctacaatatc ttcaaaattg
     6421 tctggtttta gctgtttcat aaaacttctc atgccagcac tctccaattg aaacacccct
     6481 agtgtatttc ctgatgaaag catttcatat actttcgggt cgtcataatc catctttgaa
     6541 aagtctattt tttctgtgta gttttttata actctatttt tttctattaa atcaagagta
     6601 tctcttataa ctgtaagagt tcttagccca agaaaatcca tctttaaaag cccaagttct
     6661 tcaagtgttg tcatagtaaa ctgtgttgtg attgcatctt gatgtttgta caaaggtaca
     6721 tattcatcta cgggattttt tgatataaca actcctgctg catgagtaga agcatgtctt
     6781 agcattccct ctatctgttt tgatatattt attatttctt ttgtttccgg gtcttggtca
     6841 tacaattctt taagattagg attcgtatct agggccttat ctattgtcat tcctaaagca
     6901 aatggtattt ccttggctac cttgtcaact ttattgtagc ctatatccaa tacccttcca
     6961 acatctctta ttgcagcttt tgcacccatt gttccaaaag ttattatctg ggctacatgg
     7021 tcatcaccat attttctctt tacataatct ataacttctt ctcttctctc ataacaaaaa
     7081 tctatatcta tatctggcat ggatatacgt tctggattta aaaaacgctc aaataataga
     7141 gagtacttta ttgggtctat atcagttatc ttaagtgtat atgctactat cgaaccagcc
     7201 gcactacctc tacctggtcc tactataata ttattttctt tagaaaaatt tataaaatcc
     7261 caaacaatta aaaaatactc tacataaccc attttttcaa ttacatctaa ttcataattt
     7321 aatctctcta gtatctcatt actaggatta tcatatctct cttttaatcc attaaaacac
     7381 aactccctta aataagtttc tggtgtatat ccttctggaa catcatactt aggaagatgt
     7441 atagtattaa aatcaaactc cacattacac atatcagcta ttttcacagt attgtcaata
     7501 gcctctaatg catatggaaa taattcttcc atttcttctc ttgattttaa ataaaactca
     7561 tcactaccaa atctcattct atttgggtca tttaatgttt ttcccatttg aatacacata
     7621 agtacatcat gtattttcga atcttcttta tttacatagt gaacatcatt agtagcaact
     7681 agtggtatac cagtctcttt tgataatttt actaaaccag agtttacttc tttttgctca
     7741 ggtaaattgt gatcttgaat ctccaaaaag aaattttctt ctccaaatat gtctctgtag
     7801 tcaagagcaa attgttttgc tttttcataa tttctattca ttaaagcacg tgctacatct
     7861 cctgcaaggc atgcagaaag tgctataatc cctttgttat gtcttttaag ttcttccatg
     7921 tcaactctag gcttatagta aaaaccttct acgtatgaag ttgaaactat ttttattaaa
     7981 tttttatatc cttccatatt cttagctaac agaactaaat gtccttgata tttgtcatag
     8041 tttgggtctt tatctctcaa tccacgtgca gctgtataga cttcgcaccc tataattggc
     8101 tttattcctt cttttatagc tgttttgtag aaatctataa caccaaacat acaaccatgg
     8161 tcagttatag ctattgagtt catgtttagt tccttagccc tttttataag ttttttcacc
     8221 ctcgaaaacc catcaagtaa actatactca gtatgtacat gaagatgagc aaaatctttg
     8281 ttcattaaat cacctcctgc tttataattt taattttacc acaaatatat gtcctaaaac
     8341 aaaaacagag acttttatct caaaatagga attaacttca acgtatacca ttatatgtta
     8401 taaattaatg cttattctga gattgtcccc tcaatttatt gctatgattt ttttttcaaa
     8461 tctgttgcaa tttcttctat ttttctaagt ctatgattga ctccagattt tcctattggt
     8521 ggttctaaca tctctccaag ttctttgaga gtcatatcct catactcgat tctaatctta
     8581 gctatctctt gtaaattttc tggcaaactg ctaattccta tagtctcttg tatgagctta
     8641 atattttcta cttgtcttac tgctgcattt actgttttag aaaggttagc tgtctcacaa
     8701 tttactatcc tgtttacatt atttctcatt tcttttacta ttttagtatt ttgaagactt
     8761 aaaagagcgt ggtgtcctcc tataatactc aacaaatctg atatttgttc actctctttg
     8821 atataaacaa cgtgattatt ttttctagcc acaattttac tattaaaacc taaagagttt
     8881 ataagttctt ttagagaatc tgcaaactcc tcattattag ttacaaattc catatgatag
     8941 ttcttttctg ggtcgcttat agaacctcca ccaagaaaag ctcctcttat gaaagctctc
     9001 ttacatcctt catgcttaat taagttctct gaaattttgt ttatagtaaa aaatccttct
     9061 ttatcttcaa gtatacctaa cttttttaat aacatttctg aacccatttc gcttgttacc
     9121 attaatacat aactgttatt tctctttagc atttgattct tatttactga gattgtagta
     9181 ttgatattaa aattcttttt taataattta aatactttac gggctattga atttaactct
     9241 gttgttatct ttagatttag cttcttatat ccaacaattt gtatacttcc ccctgattta
     9301 actagagctg acaattcagc tatattacaa cattcattcc cagatataac tcttgctagt
     9361 tcatttttgg tttcagttga aaaagacata tctatcacct attaactaat ttttctttaa
     9421 cctttttttt atttcttgta aggcaacctt tattatctcc ttttcatcat catacctagc
     9481 taagtctact actctatcta ggtggataaa tttagcatca ataaagcctt cctctttttg
     9541 tggaatagta tccatattct ttgcttgcat caaataccaa aaaactgttt tatgaactgc
     9601 tctattttca tcccagtttt ctttgaatgt atagtgtatt tcacctaaat atttcaatat
     9661 atctgctttt actccagttt cttcacttac ttcacgtaat gctgcctctt ggttattttc
     9721 cccttcttct acttttccct tgggtaacac ccaatctcca ttgaacttcc ttagtaaaag
     9781 aatcgtattg ccaaacagga ctacaccacc agcactaacc tcttctctca tcgatgttca
     9841 ctccttaaaa ctaacataat gataaggatt tctacacaat tttttaaaat ccttttttac
     9901 taacttttgt tgtaattatg atttagggct aactcaataa ctatgttaga tatatacttt
     9961 gcatcatgtc ttatataatt attttttatt tcaattaaat tcttctctac agtttttata
    10021 cccatctctt ttaatttatc tttttgcttt ttatctaaca agacttgctc tgctccatct
    10081 tgtttatata ggtcaagcat ttcttctggc aatatctcat tatttgcgat tacataatct
    10141 attaaattct catcagtgtg ttttactata gcattaacat gctctaatac attgtaccct
    10201 tctgtctcac ctggttgagt cattatattt gctatatata cttttggagc tacagacgat
    10261 ttaatagcgt ctacaatccc ctctacaagt aaattgggta ttatacttgt atataagctt
    10321 ccaggaccca taattataat gtcagcatca taaatagatg caataacatc ctttaatggt
    10381 ttgacatctt ttggatttaa aaaaatttta tcaatagagc ttttttgaat ttttgactct
    10441 tcaggtatac tggattctcc atttattata ttgccatttt ctaatttagc aactaaattt
    10501 acatcttcca aagttactgg taaaactctt cctgttatag caaaaatttc acttaattta
    10561 tatacagctt tttcaaagtt tccatacaac ccattcatag cagccaaaaa caaatttcca
    10621 aaactctgac cttttaaaag cccttctgta aacctatatt gcataacttc attcattgtt
    10681 ggctctatat ttgcaagtgc taatagacag tttcttatgt ctcctggtgg gagcattcct
    10741 aagtcttctc tcaatacccc tgaacctcca ccatcatcag caacagttac aattgcagtt
    10801 atattttgcg tagtgtgttt aagacctctt aaaaataccg attgaccagt acctccacct
    10861 atcacaacca catttggttc tctattttca acaatagacc tttgaaatag ttttactttt
    10921 tcattttttg cttttttata tataaataac gatacaatta atgcaatcca acctaaaatt
    10981 cctacaataa taagtaattt agccatgttt gcctccatta tcgcaacata gagtctctat
    11041 gtttctttac aactctatat cccttattag ataaatcctc cgctataaga tttgttatag
    11101 taactgacct atgtcttcca cctgtgcatc caacaccaat tactagatgt tgttttcctt
    11161 cctctatata ttgaggtact aaaaaatgaa tcatatctaa aagtttcaca taaaattctt
    11221 cactgatttt agaattcata acataatctc taacttcttt atcatcacca gttttagctc
    11281 ttaactcttc aacataatag ggatttggaa gaaatcttac atcaaatact aaatctgcat
    11341 cagcaagtat accatgttta aatccaaaag atactacaga aatagttagg tttggattat
    11401 cttcaccaga agaatatatt ttctttattt cttccttaag gtctttaggt ttcatgttag
    11461 ttgtatcaat tatacatgta gataactcct ttaaaggttc cattatcttt ctttccattt
    11521 ttataccttc tggaatctgc atatcttttg ccaatggatg atttcttctt gtcattttat
    11581 atctctttaa caatacatca tcattacaat ccaaatatac catttcatat tcataatttt
    11641 ctttttctaa ataatttaag ctttcatgaa gcgcttcaaa aaactttctc cctcttatat
    11701 ctatccctag agcaacttta tcaatacttg aatttggttg ataacataat tctgcaaatt
    11761 tagttatcaa agcaggaggt agattatcta cacaataaaa tcccatatcc tctaaagctc
    11821 tcattgtctc actttttcca gatcctgaaa gacctgtaac tataacaaat ttcattgcta
    11881 actcctccct tttatcttat gtttattata ttataatgtt ttctactaac gatatatcca
    11941 gattagagtc agatatagtc ttcatagcta gttcaaaatc accaactctt tctggacaat
    12001 gtgcatctga gcctattata aatttatttt ctagcccttt tattttattt aattgattta
    12061 cattcaaaaa agaatggcta ctatttatct caagtctagt atttgtcttt tttgctgtct
    12121 ttgctacttc ttctatatac acatcgcctt tatctcctgg atgagtaatt ataaaaatat
    12181 cattattact tatcgcattt attacagctt ttgtattata ttcttttgct ttttcagttt
    12241 taaaaatata gttattacaa tgatttaata agcttcttaa tgatgttgga gtagaaccaa
    12301 agtgatatcc agccataata taatcaaaat ctctaataaa tttatcatcc atatctatat
    12361 ttcctttatc atctaatatg ttacactcca tacctaaaag tatatttata tcattgtact
    12421 tatcatttaa ataatctact tcttctctca ttttgagaat atcttttttc tttacaccat
    12481 atgcaatatg tttataacta tggtcagata ttcctattgt ttttattcct ttagaaatgg
    12541 ctacctttac attatcctca atagtccctt tgccatgact atataatgta tgtgtatgat
    12601 aatcagccag tattttcatt tgttaatcct ctccaagtat tttgacttct tcttccaaca
    12661 taaccccaaa cttggcatat acagcacttt ttataacata tattaaatcc aaaatatctt
    12721 tagcacttgc ttctccttga ttgactacaa atccacaatg cttctcagat acttgtgctc
    12781 ctcttaaagt aagacctctc aatccagttt cttctattaa tttacctgca aaatatcctg
    12841 ttggtctctt aaatgtactt cctgcactag caaaatttaa tggctgttta gttattcttc
    12901 tcgtcgctaa atcatccatt aaacttttta tttcatcata atttccttct tgtaattcta
    12961 attctgctga taaagctata tatccgtttt tcgatattat actttttcta tacccaaatt
    13021 ccatctgttc atttgataat tcaaatatat tgccttccat atccatcagt cttactgact
    13081 tcactatgtc cttcatttca ccaccatatg ctccagcatt catggctaat gctcctccca
    13141 atgtgccagg tattcctgat gcaaactcaa acccttttaa ttcttgcttt tggagagctt
    13201 tccctaaaac tgataataaa gctcctgact gagcagtcat cctagtatca tttatctcat
    13261 aagaattgaa attatcagct agttctatta ccactcctct tattccacca tctttaatta
    13321 aaatgtttga accatttcca attattaaat acggaataga ttcttctttt accaacttaa
    13381 gtacattttt taattgttct tcagttctag gtctaactaa tatatctgct ggtcctccta
    13441 ccctaaatga aatatgtttt ttcataggtt catcgacttt tatatcctct ttacttaata
    13501 tatttaataa atttatatat atatcttgat tattcattgt atttcctccc aaattatact
    13561 gaactttagc tgtcattatt atttaaatta cattagtata tagtattaac tatattcaat
    13621 aatatatgat tttgcttatc atatggattt tatattttaa gcttcttaat tacaaactat
    13681 attcaataaa tatataataa agtaaaaaaa aaaaaataac aacatataaa agaatataaa
    13741 taatttaaaa aaagcatcaa tatcactgat atcaatgctt taaaaggcaa tttaattcgt
    13801 attatttatc taactatttt tgacatagca aagataataa atccctccaa ctactatgga
    13861 acctccaacg atatttccaa gtgttacaaa taccaaatta gttataaatt gaaccatagt
    13921 tatatttgct cctaatgcca ttcccattgg tataaaaaac atgtttgcaa cactatgttg
    13981 gaatccacat aatacaaata gcattattac tgtccaacat gaagccaatt ttgacattat
    14041 atcttgagat gctgtagcca tccatacagc taaaacgact agaatattac aaagtattgc
    14101 ttttaaaaac attgatgcta tagttgaatt aaccttagct tctgccacag ccatagcttt
    14161 tgtagctgca tctcctgtca atgtagaaga ataaaacaca agtgcaacta gcaaaattga
    14221 acctactaaa tttgcaaaat aaaccagtcc ccagtttctt aacatttttc ctaatgttat
    14281 ttttttgtcc ataagtgcaa tagccattaa ggtgtttcca gtgaataatt ctgctcctgc
    14341 cactactaca agcataagac ctactggaaa gaccatagca cttaaaaagc tagctacccc
    14401 tgggtctatc ttgccaaaag tttgactaac tatcatactc cccaatgccc caaaacctat
    14461 aaatgctcct ccaaataaac ctaatagaat tgcattttgt gtcgaaatat ttgctttttt
    14521 aatgcctgca cttatagttg cttgtgcagt ttccccaggt gtcaagaatc ctttactcat
    14581 aaatccttcc cccttaaaaa ttatactttc ccttgtcttt ttatggcaat aattatataa
    14641 taacaatatg atatataata aattttatta acataaaaaa tatactatat gcttatatat
    14701 acttgtctca tataattata ctatttatta tataacaact ttgttaattt cgaaataatt
    14761 tatattgaat ttgaaaaatt taccttttga ttatttattt tactttttaa ttattgaggt
    14821 attatatata atagatatat gtttgtgtta ttatatataa ttacttattt taataaatat
    14881 ttgaaggagg gaattttatg tttgatatat catttttcga aatgtcaata tatgaaatag
    14941 cagttactac aatatatata attaatttca tggttatact aaaccttatc tttagagaaa
    15001 aaagaaacat aaatacaact ttaacatggc ttcttatatt agtattgatt cctgctcttg
    15061 gattcatact ttatatagct tttggcagaa atatttcaaa aaacaatatg tttagattga
    15121 aagaaaagga tgataagata atcaaaagta atatactaga tactcaggtt aaactccaat
    15181 ctacatctga aattgattca gatatacatc aacataagga tatgatttat gcattggcta
    15241 attcaaataa tgctcattat accaataata acgatgtgtg gatttatgct gaatctagtc
    15301 aattttttaa cagtcttctt gaagaactta aaaaagctaa aaaatatata aacatacaat
    15361 tttacatttt caaagatgac aaaatcggaa ctgaaataat agacattctt atagacaaag
    15421 ctaaagaagg tgttgaagta agacttttat ttgatgcagt tggtggtaga acactaaaaa
    15481 atagtacact ttccagactt aaagaaagtg gtgttaaagt tggaagcttc ttcccatcat
    15541 tcttgaaaat tgtaaacttt aacttaaact atagaaatca ccgaaaaatt gttgtcattg
    15601 atggtaaggt aggatttgta ggtggatata atgttggtga tgaatattta ggtagaaatc
    15661 caaaatttgg tctatggaga gatacacata ctaagcttac tggtgattgc gtaattgacc
    15721 taaatatgag attcatcctt gattggagat atacaactaa agaagattta gatttagaaa
    15781 agtactttat agaagaagat acaaattcta atcatccttc tgaaaatatt gggattcaga
    15841 tagtatcaag tggtccagat atatcagaac ttgatgagat taaatatggt taccttaaaa
    15901 tgattcagaa agctagaaag tatatataca tacagagtcc ttatttaata cttgactcaa
    15961 cttttataga tactttaaaa atagcttgtt tatctggtgt tgatgtgaga gttatgattc
    16021 cttctaaacc tgaccatccc tttgtatact gggcatcata ttcatatgct ggagaacttt
    16081 taaagtttgg agctaaaatc tatacttatg gtcaaaatgc atttttacat gctaagacaa
    16141 tagttataga tgactctata tgctctatag gaacagctaa tatggatatc agaagttttg
    16201 agcttaattt cgaagttaat gcatttatgt actcttcaaa aaaagcattg gaacaaagaa
    16261 ttatatttga aaatgatatt ttaaattcta aggaaataac attagatata tataattcta
    16321 ggtctactta tataaaaatc aaggaatcaa tttcaagact attatcagca g
//
LOCUS       DAEMHW010000080        16335 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEMHW010000080 DAEMHW010000000
VERSION     DAEMHW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.080"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            269..2502
                     /locus_tag="KPK67_002120"
                     /pseudo
     CDS             269..2502
                     /locus_tag="KPK67_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            2533..3378
                     /locus_tag="KPK67_002121"
     CDS             2533..3378
                     /locus_tag="KPK67_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2459088.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3618..5351
                     /locus_tag="KPK67_002122"
     CDS             3618..5351
                     /locus_tag="KPK67_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2459089.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGEDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5732..6155
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5798..5823
                     /rpt_unit_seq="ctttacataccacatagttaatataa"
     gene            complement(6435..6587)
                     /locus_tag="KPK67_002123"
     CDS             complement(6435..6587)
                     /locus_tag="KPK67_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459090.1"
                     /translation="MDNFLQGVLASLVASLIVYLNSKLFKKVKSHSGRSDFSFELKIK
                     FKKNKH"
     gene            6862..7722
                     /locus_tag="KPK67_002124"
                     /pseudo
     CDS             6862..7722
                     /locus_tag="KPK67_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454819.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            8264..9619
                     /locus_tag="KPK67_002125"
     CDS             8264..9619
                     /locus_tag="KPK67_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2459091.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(9737..10405)
                     /locus_tag="KPK67_002126"
     CDS             complement(9737..10405)
                     /locus_tag="KPK67_002126"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBG2459092.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(10472..10900)
                     /locus_tag="KPK67_002127"
     CDS             complement(10472..10900)
                     /locus_tag="KPK67_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG2459093.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            11007..11600
                     /locus_tag="KPK67_002128"
     CDS             11007..11600
                     /locus_tag="KPK67_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBG2459094.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            11719..12654
                     /locus_tag="KPK67_002129"
     CDS             11719..12654
                     /locus_tag="KPK67_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBG2459095.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(12925..13869)
                     /locus_tag="KPK67_002130"
     CDS             complement(12925..13869)
                     /locus_tag="KPK67_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG2459096.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKVCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            14199..15629
                     /locus_tag="KPK67_002131"
     CDS             14199..15629
                     /locus_tag="KPK67_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2459097.1"
                     /translation="MKLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFTEDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            15829..>16335
                     /locus_tag="KPK67_002132"
     CDS             15829..>16335
                     /locus_tag="KPK67_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBG2459098.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTC"
BASE COUNT         6271 a         2145 c         2145 g         5774 t
ORIGIN      
        1 tctatttatg aaaaaatatg atataattgc attcaaaaag gaaatctttc aatatactag
       61 aatttaagca ttaactaatt ttatctaata ttattactaa actaaaaaat atttgtattt
      121 gtagcattgg agcaagtaac ttaatgtata ttcattttaa atacttattt aaaatttggc
      181 atttaaatta tttacaaatt aacatttaaa tacttctagc ataaatacag ttgtcatcaa
      241 aatttaaagt atcttatgga ggaataccat gcaaaatcat aattatataa aaatgttaaa
      301 taaaaaggta gaagtattat ctaaggaatt agatagtaga aattttaaat taaaaacctt
      361 aaatgaaata gatttttttc taaaatacca aacagagaat ttatgtacca atatattgat
      421 gactgtattt cgaaaaatat acatatagga atcatgttat taaatatcga taactttaaa
      481 ttatttaatg atatatatgg atatctagtc ggagaaaatt tattaattac tctaactcaa
      541 aaccttttgc actttattcc agaatcaaag ggaatagtcg cttatttaaa aggagaaggt
      601 tttttaattg tacttccaaa tgtatcaaat aaagagttaa ataatatggg tgcaactatt
      661 atagagagcg cccgagaatt aaaaataaga cttcctaatg aaaaagaaat atactccaat
      721 gttacattta gtatcggcag cactatctgg aatggtgaac caaacatgac tactttgatg
      781 cttttcaacc aagtatatag tgctttaaaa aaggctaaaa gtgaaggtaa aaatagatac
      841 attctatttg attttaaaga taatagtttt tacaataaat tagaaaatga taaggagttc
      901 ttatcagaaa tatcacatgt tctacaaact aaacaaattg aagtatttta ccaaccactt
      961 tataatataa aaaataatac tatagttgga tgtgaggcac ttgcaagatg gagacaccct
     1021 attaaaggac ttcttactcc agataaattt attcctttat ttgaacagtt tggtctgata
     1081 acggaattag atttgcattt atttgaagag aactgtaaaa atataagaag atggattgat
     1141 aacaatgatg tttttgttcc aatttgttgt aacttttctc gtcttcattt ttttaaccct
     1201 aattttccaa gtatgctaaa gggtattact gaaaagtaca gcgtctctac atcaaatttt
     1261 agcattgaaa taacagaaaa tatattaata gaaaatacaa atacaattat tcaacaacta
     1321 gctgaacttc gttcttatgg tttttctgta tcaatggacg attttggttc tggatattca
     1381 tcttttggca tgttgcaaaa tctacctttt gatataatca aaattgatag gatattttta
     1441 tctcataatt taaatgattt taaaaataca accattttgt attctataat aaacattgca
     1501 agagctcttg ggatgcttac agtatgtgaa ggtgttgaaa cagaacaaca tgtagaattt
     1561 attaaaagca taaattgtga tatagcccaa ggttattatt atgcaaaacc catggagtgc
     1621 aaagaatttg aaaaattact atctaaagag aataattcag agaaaatgtt tataaaatat
     1681 tctgatatag gaaaatcttt aatagagaag atatttgatg gattttttat tatgcaagat
     1741 atgaaaaaac taagagctaa tgtcactgaa gacattcaat ggtttgattt ttacaacaaa
     1801 actgaaatta catcttttcc taaagtaata gaacattttg aaaaacatat taaaggtaaa
     1861 aaattttcta ttctttacaa aagtataacc ccacataata ctgaatcaga ttcattatta
     1921 attagtggag aagccgtttt atcagatgaa gcttctgaac atcaaaaata tagcaatttc
     1981 tactttagtg caaattgtat aatttcagaa aataaattac tcttagcaaa attacatttg
     2041 gatttaatta aaactgctgg ctatataaat gaatttgaat catattcaat acctaaaatg
     2101 acaaatacac atcttgatga aagtaatatt ttatcacaat tttattcaat gttgcctgta
     2161 ggtataattc gctacgattt atttggtgat atgattatta cttatatgaa tgaagaaatg
     2221 tttgatatta taggttatac aaaagaacag ttttttggtg agatgggcgg aaatttaaga
     2281 gcaattgttc atccagatga tttagacttt gtttataaaa aaagtttgga tatgattgac
     2341 acaaataaaa ttgaaccatt tttatataga ttcataaaac gagatggtca aattgtcaca
     2401 gttatgtata cacaatgtaa cctttctgca attgatggaa ggtcgattgc ccagggtatg
     2461 tatgtagatg taaataaaat aaaaaattta acaaaaatct agtacaaagt attttatttt
     2521 ggagaaatta atatgtttaa agaaattttt ttaagaactt ttccaggatt tctaattata
     2581 agagattcta actacagaat tatatttata aatgataatt taaaaaattt aattaaaagt
     2641 tatatgcaag ataatccact tggaatgact aatatagaaa tagctaagaa actccctgat
     2701 aatattgcta aattttttac ggattctcac aatattcaat tagattggga aaaaaattat
     2761 ccttatgata aaatttcaaa ttggatttta gagtttaaaa aagatactac aagctattgg
     2821 aatgttttag aatacaaagt agatgttgat gaaaaaacct acataattac tatggccaat
     2881 gatataacaa aactctatga agaaaataag agaaatcttc actactcaat tactgaccca
     2941 ttaactggag catataatag aaaatattta aatgatagat ttgacatatt tataggagat
     3001 tatattgtac taattgactt agataatttc aaaatgataa atgactatga aggtcataat
     3061 gtaggagaca agattttatg tgattttgtt tcacttttaa ataaagaatt aataaattct
     3121 acttctatta taagattagg aggagatgaa tttattgtta tattctctag tgatgttgac
     3181 aaaaattatg tttattcaca gctagaagcc ttaagagaaa attttttaaa agtattctct
     3241 aagtataagt atctttcatt tagttatggt gttgatacag taaaaagaaa tttaaaattg
     3301 acaatagttg aattggataa aaaaatgtat aaaaacaagg agaaaaacaa gaaaaaattt
     3361 gataaaaatg attattaaca ttgttatttt aaaataaatt aaaattatct ttcttttttt
     3421 aaacttgtac attagtttat aaatatgtat ctatacaaga tattgacatt aagtactgaa
     3481 gcatatattg tttcagttct tttttgttaa aattaactta tttaacaaag atatcatagt
     3541 ttaacaaagc ttttaattta aatatattga tgtataatat aattaaaata taatagcaaa
     3601 aagaaaggag gaatatcatg agcatactcc taaaaaaagc acccaaatta gcaaaacata
     3661 ttataacaag tttttatata aatcgagata tcaatgaagt attaaaatac ctttgtgaaa
     3721 atgttacatg gattggacca ggagaacagg aatttctcac ttcttttaat gaaataaaaa
     3781 attattttta tgcaggtcaa gatgaaattc cttcttgtga tataaataat gatatatttg
     3841 aaactgtaag tgaagatgag aatagatgta tggtattagg aagatatact gttcgaacta
     3901 aagaaaatgc tcaaatgatt ttagaggtaa atcaacgttg tacatttgag ataattgagg
     3961 atagaggaaa attattaata aagcacatgc acatatcaaa cccttatggt gaaatgcaac
     4021 ttgatgaata ctttcccact aaaattggaa ctcaaagtta tgattatttg caaagattac
     4081 taaaagaaaa aacagaagtt attgaaatga ttacaaataa tatcaatggt ggcttaaaag
     4141 gtagtaatga tgatagtaca tactcatttt tctatgtcaa tgagggattg cccaaaatat
     4201 taggatacac atatgatgag ttcatggaaa tgagtggtgg ttcagcagtg ggtgctgtat
     4261 atccaccaga cttacctaaa gcactagaag attgtgaacg atgttttgca aaaggaccta
     4321 catattcatc tgaatataga atccgaaaaa aagatggaac attaatgtgg gtattagatt
     4381 ctggaatgaa atcgttaaat agtgatggca tagtaaaaat taatagtatt attactgaca
     4441 taacccaact aaaaaacata gaatctgaac taaaattaga aagagagcga tatcgtattg
     4501 ctttgcaaaa tattacagat atcatgtttg agtatgatat tgaaaacgat acccttataa
     4561 aatatcaacg tgtagaaatt gacaaaaaaa ttgaattaga aaattttgaa acaaaaaatt
     4621 actccaaact attagaatct ggtaaaatta ttcaccttga tgatattgga aaactactag
     4681 aagtacttca tggaaatctt cgtgaaacaa ttgagataag acaagtaaat tcattaacta
     4741 aaaatgaatg gagatggatt cgtgttcaat gttcagttat atatgatagt gaccataacc
     4801 ctatcaaaac aataggcgtt ttaaaagaca taactgaaga taaatctaaa ttagaaagct
     4861 taattaatca ggctcaacgt gacccactaa cacaattata taatcaaaga gtatcacaaa
     4921 atcttattga agagtatcta tgtagttctg attccaaaaa taatgatgct ctattagtta
     4981 ttgatattga tgattttaaa actgtaaatg atacttttgg gcatttagag ggaaatgaag
     5041 ttttagttgc tgtatcaaaa atactattac ataacacaca tgataaagac atagttgctc
     5101 gaattggtga agatgaattt acaatattta taaaatcatt aacaaaggat ttaataataa
     5161 aaattactaa tgatatttta aatgatgcaa gtaaaataaa agtaaaggac aatcataaaa
     5221 taacattaag tattggtatt gccttcactg atgattcaac taaattatat aaagatttat
     5281 tttctaaagc tgataaagct ctttatctat caaaagcaga tggcaaaaat tgttatagta
     5341 catacgaata ataaagctat ttatacgacc aagaatgaac ctttaaattt aatttcatag
     5401 tctaacaatc aaagagttga agctaatctt atagctagtc aactagttcc tagaaaaaat
     5461 ttagacaata tatttattga tactgctcat ttttctaaag agcaaattta tataaaaaat
     5521 taaactttta tgtacataca gatactaatg ataaacagtt atcattagta tcttctatct
     5581 taaaacaaaa tgataattta tctatataaa aaagtgcttt gttgacagag ttcacttttt
     5641 tatataggta cagaatttaa gtataaatac atgttttatt ctatacattt taatttcttg
     5701 attgtactct ctaacttttc aacattatac tatttacata cctcttagtt aatataaaac
     5761 tattaaattc aaatccagcg agaggtaaat taaaattctt tacataccac atagttaata
     5821 taaaccgata cagccgttcc cgagcctggt atatatttca attctttaca taccacatag
     5881 ttaatataac ccacgcccaa ataaacttca ttacttttaa ctcagaatcc tttacatacc
     5941 acatagttaa tataacccca ttgatgctaa cttgagtatt ccttgttttt gtgactttac
     6001 ataccacata gttaatataa cccaatggga taaagaagaa tgttggataa aagctaaccc
     6061 ctttacatac cacatagtta atataaccct ctttttcctt tttcaatttt aagtcttttt
     6121 tgtatccttt acataccaca tagttaatat aaccccccaa aataaactgg gcattttcaa
     6181 tgcttacaca tgtgcaactc tcttaaattt gcagtgaacc atgagtagtg caaatgataa
     6241 agcttatcac atgccctcaa caccttatat ttcaagcgtt aagccctact ttgtaacaaa
     6301 tatcgctcac tgcaaaacta tttacatttt tattatatca taaatatatt atttttgaat
     6361 atctgtacca atttgtggta taataaaaac aagaagaact acaatctatt tagacgtaga
     6421 gtgaagttca aatactaatg tttattcttt ttgaacttga tttttagttc aaaactaaag
     6481 tcactcctgc cagaatggct ttttactttt ttaaataatt tactatttaa gtaaactatt
     6541 aaactggcaa ctaaacttgc tagtacacct tgtaaaaaat tatccataca tactcacctc
     6601 ccttctatac attgggagga taatcttttg tatgaactcc actctataaa ttgtagatta
     6661 catcttcttg ctaaaaatgt tatagcatat aattcttaca tattttacct atatattact
     6721 tttctaaaga ttcgtctata tatttctgta tacatctact ctatcctcga atagaattta
     6781 taacttagat ttgatataat aaaaccatag ttattcaata tattttaaac atgaataata
     6841 cttataaagg agtgtacata tatgaatatc aaatcagctt ttataagaaa aagagggggg
     6901 aatttcatgt atatgtggaa aaagaaactg gcaaaaagaa acagaaaagt tatggaaatt
     6961 atgaaaagaa aaagtatgct gaaaaacatt taatttgaat aaagtccact ataaacaata
     7021 ataagtttgt tgctccaaat gatgtaacac ttgtagaaag atgttacaga tatataaaag
     7081 atagagaatg tagtttttct cctaatacta ttagaaatag aaaaaatatg attaaaaatc
     7141 aaattgaagt tttctttaaa gatatgaaat taatagatat aacaccttct attttacagt
     7201 cttatataaa taacatatac aatgagcaca tgttaaattc aataaaaaac caagtcgatt
     7261 ttataaaatc agtattgaaa gaatcttata gattaaaaga aatatcagaa aatatttgtg
     7321 attttgttac aaccccaatc aaaaaagtag ttcaacttct aagttttata caaagcagga
     7381 aactcaatta ttattagaaa aatcaaaaaa tactcctata ggtataccaa tttttttgat
     7441 gctaactctt ggattacgtt ttggtgaagc tgtatcactt atatggtcag atgtagacct
     7501 tgataaaaaa tctcacatgt aaatcaaaca ttagtttatg tatacaataa aataatcttc
     7561 aaagacccta aaacccctat atccaaaaga aaattatttg ctcctgatga acttataagt
     7621 ttgcttaaag gggaaaaatt aaaacaaaat aagttaaaat tacagggtat attgaaaaat
     7681 gaatttgatt tgatatgctt aaataaaaga ttcaacccgt gagttcaacc aacttttgtt
     7741 gttcatttta aaaaactatt aagagataat aatcctagag atataaaagt acatgaatta
     7801 atacatgcaa atgcaacttt gatattactt tcaggcacta acataaaagc tatttataaa
     7861 atattagtac atgcagatat aaaaatagct atgaatatat attccatatt ctcacttatt
     7921 agatgaaatg gataaagaaa catctgaaaa tataagcaaa atactattca aataatgttc
     7981 agttttatgt caattaaatg tcactcaaag tgcttttttt ccatttgtca gttaaatgtc
     8041 acttaaagta cctttttcct atttgtcagt taaatgtcag taatatataa ataagatata
     8101 tttataagac ccttaaaatc actattattt aataaaattt ataattatat actatttgct
     8161 gttgacattg gtattatact atagtttatc atttatgtta gaaataaaat tattaattaa
     8221 tagtttcgta attttataat agatttacag ggagtgataa taaatggata caaaaatatc
     8281 tataaacgaa aacatgagtc taggtaaaag attttttaaa tacctagcac catctgtagt
     8341 tgcaatgtgg gttttctcac tatatacaat ggttgatgga atatttgtaa gtaaaggagt
     8401 tggagaatta gctttagctg ctgtcaatat atcaatgcct tttattaatt ttatatttgc
     8461 agtttctcta ttattttcaa ctggtgcatc taccataatt gcaatctatc tggggaaaaa
     8521 agatattaaa tctgctaacg aagtcttttc atttaactta gtatcaataa ttatattatc
     8581 aataattata ttagctataa ctttttttaa tttagataga ttagcactat ttttaggagc
     8641 tacagaatct acaattggta tggttaaaga ttatttagga ataataatat tttttaacgg
     8701 attctttata gtgtcttatt ctttagaagt tataataaaa actgatggct ttccaatatt
     8761 agctactgtt ggtgtaataa tatctgctct tacaaacatc atacttgatt acttgtttgt
     8821 aattgaattt ggatggggtg taaaaggtgc tggtattgca actggtttgt ctcaagtctt
     8881 ttctactatt ttcttcttaa tacatttttt aagaaaaaac tctactttga atttttcaaa
     8941 atttagaatt gattttaaga cacttagaaa aattgtattt ataggttttc ctgattcaac
     9001 tactgaatta agttgtggta tagttgtttt actttttaac ttaagtctta ctaaatatat
     9061 tggagaaaat gctcttattt attatagtgt cataaattat ataaatacat tagtacttat
     9121 gactatgatg ggtataactc aaggtatgca accacttact agcttttatt atggtgcagg
     9181 aaatatagat aatgtaaaaa aattattaaa aatggggata aaagccacaa taatagcatc
     9241 agttgctgta tttgcaattt gtatggcttt ttcaggacca atagtctctt tatttataca
     9301 tccagaagaa acaatgctat tcaatgaagg agttcgtgta tttaaaatat tctctatatc
     9361 tttcttattg gtaggaatta atgtaataat ttctggattc tttgtatcag tagaaaaacc
     9421 atcaatttct actgttatat ctcttggtag agggcttgta atagttgttt taagtctaat
     9481 ttcaatgata ctaatatttg gtggacaagg aatatggatg actactattg tttctgagtt
     9541 tatatgtctt attttatctt tagtttttct aaaaaagaat ttttctacat tagactctaa
     9601 tctaaataag gttgcttaga tgataagagt agcaatatat gaaaatatct aaataaaaaa
     9661 tacctattat acaatcttgc aaatattaaa cctttgcaaa aattaggatt atataatagg
     9721 tattttattt atgtaattat tttatttcta aatatccata gccatctttt attaaagttc
     9781 cttttacttc atattctaca ggttttttaa atattggacc atctacgaca aatgtatgat
     9841 actctccatg ttctccacat gggtctgcac cagcattttt tatcttctcc acaagttctc
     9901 tagttaattt ttttcctaaa aattcctctc ctaagtgctt taagtttact tttgttacaa
     9961 cactacaaaa tccactgtct ataaattcat atactaaatc ctctctactc tcttgccata
    10021 atggtaaatc tactttcata ccaactgctt cacatctatc agtatcccat tttctatgac
    10081 tttctatatc tatatctcca tatgcacata tcgttgctcc catgttttta gcttttatta
    10141 aagccctctc aaagctctct tcatatcctt ctacaccaca ctcagcaact aataaaggta
    10201 atcctatatt gctacttact tgcttaagca tattatattc taaattatgt gtccatgact
    10261 ttccatgttc ttcacttata gttgtcaaga gtgccacagg cttatatcct tttttcaaca
    10321 ttcgatttaa tgctaatata ctatccttac caccactaaa tgacattaca aatttttcat
    10381 cagccaattt taacacctct ttcatctaac tttgttttat taacataatt ataccaaatt
    10441 tttgagttgc atagcaaatt atattaaatt gttaattcac tataaagaat gtccctaata
    10501 ttaaaagtat aacccctatg tatttgtata aatgcattgt ttcttgtccc aaaattacgc
    10561 tatcaataaa aaatccaact aaaacttggg atacaagagt taatataagt gtatttgata
    10621 ctcctaaatt tggaattgcc tttactgtaa aatatattat tagacctcca aatactcctc
    10681 ctaataacaa cttataatta aaaccattta tagatactag acactttaag tcacctgtaa
    10741 ctattatact tgccaaaaaa aatattgctc ctacagttaa gcttataaag gtggccacta
    10801 atgctgttgt actttttcct agctctccat ttataaaagc ttcaagggct gtagaaccac
    10861 ctgctaataa tgcaaatatt attgcccata attttgtcat ggacaaacct ccttctatat
    10921 aattctatta atagaagttt atgatacaac ctattaatta attactaagt atagtttaat
    10981 ttttatacac taggctttat ttggatatga atttctgtta taattgttct atcgattttg
    11041 gaggtgattt tatgtacaca aattttaata tcgattttaa taactttaat aaaaaagaaa
    11101 atgctattaa atttatgtta atgggagtat tactcattat attaggatta ttatgcctta
    11161 catttaagac attaggtata aaattaattt cttggacttt tggtattgca cttttattct
    11221 ttgcatattt aaatttaaaa aatattaatg aattaaaaag atatgctact aaagaagaaa
    11281 ttaaaccctc tattaatatc caatgggttc taataattgc atgtatatta ctttttgtat
    11341 ttcctcaaaa aatacaaagc atattttcat tatttttagg attttattta atctttaatc
    11401 aactagtagc acttgtaaat agtaagaata atccttattc taaatttaca acttggaata
    11461 tagttaagat tctatttggt atatgcctta tattatctcc acttttttta tctagattta
    11521 tagtatcgat aatgtctttt ttcataatat tatttggact agttctattt ttctctggaa
    11581 atacagcgag aaaatattaa ttattttctc gctgtatttt tatattactt aaaatagaat
    11641 ttgtttaagt tatctatttg tggtaatatg ttttttgtac aagctcagcg tacaatttta
    11701 ttagaaatag gtgatttttt ggataaattt aaatatgctt ttgataataa aagatatcat
    11761 acttggaatt actatcttag aaatactttt ggagaaaaag tttttaaggt atctataaat
    11821 gcaggattta cttgcccaaa cattgatgga agtcttggtt atggcggatg tacttattgt
    11881 agcaaagaag gttcaggtga ttttgctgga aaccctaaag ataatcttat cagtcaattt
    11941 tataatataa aagaaatgat gttaaaaaaa tggcctcatg caaagtatat tggctacttt
    12001 caagcctata caaatacgta tgctcctctt gaagttttaa aagaaaaata tgagactata
    12061 ttagaacttg aagatgtcat aggtctttca atatccacaa gacctgactg tttgccagat
    12121 gatgttgtag aatacttgtc tgaactaaat aaaagaacca atttatgggt tgaacttgga
    12181 cttcaaacaa ttcatgattc tacatctaaa attataaata gaggtcatga ttataaaaca
    12241 tttgttgaag gtgttgaaaa attaaagtct aaaaatataa aggttgtcgt acacattata
    12301 aatggtcttc ctggtgaaga ttataatatg atgatggaaa cagcaaaggc agttggtaaa
    12361 atgggtgtag acggtataaa aatccatcta ttgcatgtta taaaagatac tcctatggaa
    12421 aagatgttac aaaatggtat gttaactctt atggaacaag atgaatatat caatcttgtc
    12481 tgtgaccaat tagaaatact tcctgaaact atgataatcc atagactcac tggtgatggt
    12541 aagagggatg aactagtagg tcctatatgg agcctaaaaa aatgggaaat tttaaatcaa
    12601 attgatgata ctttaaaggc tagaaattct tatcagggct gtaagtttgt ttaatacaaa
    12661 ataataacta tagaaatggt tatataaata tagtcctttc tatagttatt tagtatttac
    12721 aaaacaaaat attaatttgt acatttatac aatatataat aaaaaataga tagtctaaac
    12781 tgtatctact tatattttat ttgtactgtc cactttattc tataaaattt ataaatagtt
    12841 attttttaat accaaatata atttggtata atttttgttg gactctaaaa tgtataccaa
    12901 taaatccttt ttaagtatat aattctaaat actttcagtt ttttcaatct gctttaactt
    12961 gttggactta taaaatattc ttatttcatc ataagaaaga ttaagtgcta ctactgcaga
    13021 tattaacatc aacatcactc caaataactt ccctaaggaa aaactttctc ctaatatagt
    13081 gcaaccaatt attactgaac caactaattc tactgatgcc acaacacctg caactgacaa
    13141 ctctacacct ttagatatac ctgctgcata aaaaatatat gcaagtgcag ctggaacaat
    13201 gcctaacatt agcatacaag ataaaacatc taaatcctta gcataattta agatttccca
    13261 aggtcttgaa gaaggaatca taaatatagc tccaaataag aaactgtata ctaatattgt
    13321 tgaacttgaa aattccttta aagcattttt actaatagtt ggcataagtg catatgctat
    13381 tgctgataat accccaagta aaagtccaaa agcatttaaa ccttgaaaat ccaaacgtcc
    13441 accagtcact gccataattg caccaataaa acaaagaata agtgaaaata atttgcttct
    13501 agtaatattt tctttataac atacttttga aaatattgct aaaaacagtg gtgatgtata
    13561 tagtaaaact gctgcaattg atacacccgc aatgtcaatt gctttaaaat aacataaatt
    13621 aaacattgcc tgacatataa ttcctattat tactgaatat ataattcctt ttttgcttat
    13681 ctttaagatt tgtggtgtcc ttatagatga atatacgcct aaaactataa atccaaaaaa
    13741 caatcttgta aatgctactt gttctgatgt cagtccattt cccattaaaa actttccaaa
    13801 taatccaagt gtagcccata ataaccctgc tattgctata aatacatacc ctttactttt
    13861 ttctcccatt ttaaattccc ttctattttt atttagtcaa tttacactct tctttttaag
    13921 caaaaactat gccatcttaa accaatatat atcgactcta aaaccctagt ctttccaata
    13981 atactaataa attttcaatt tttttatatt tatttttcac ttactttaag ttgatttaga
    14041 cttattttaa ttgtaaaatg agttatatat aacttattta aagttaaaat agactgaaac
    14101 ttatatataa atatcaaatt ttaagtatat taatcaatta aacatatttt aatatatcat
    14161 taaaaattta ggaggaaaat ataaaaatga atttaaattt gaaattagat ttggaatttt
    14221 atcaaaaaat attagaagct tcccatgatg aaatatgtgt ttctgatgat aaaggaataa
    14281 taatatattg taataaagca tttgaagaaa attatggtct aaaaaaagaa gatattttag
    14341 gaaaaaatgt ttctttttta gaagactctg gatattcaac aaaaagcccc ataccaattg
    14401 tattaaaaac aaagactaaa ttttcactgg aacaagatac tcaaacaggg aaaaagctta
    14461 tcataactgc tacccctata tttgatgaaa atggaaactt agaatttact gttgaaaatt
    14521 gtagagatat aacagaactt aataatataa aaaataaact tgaagacaca aaaaaacaag
    14581 taaaaaaata taaatctgaa gtagaaacac tatacagaac tgcacttaga attgaggata
    14641 ctgtaattat ggacggaata gttatgagac caatcataaa cacagtaaat catgtttcta
    14701 aaacggatgt aagtgtactt ttattgggtg aatctggaac aggaaaaagc tcacttgcta
    14761 gatatataca ccataacagc aatagatcta atggcccatt tataacaata aattgtgcaa
    14821 ctatatctcc acaacttctt gaatctgaac tttttggata tacttcaggt gcttttacag
    14881 gtgcaagtac taaaggtaaa gttgggctcg tagaattagc taatggagga actctttttt
    14941 tagatgaaat aggagatata ccacaaaatc tacaggcaaa atttttgcaa ttaatacagg
    15001 atagaacttt tactccagtt gggagcttaa aaaataaaaa ggtcgatata agaattatat
    15061 ctgctacaaa tgttgattta gtttcaaaag taaaagaaaa aaagtttaca gaagatttat
    15121 actatagatt aaatgttata gaaataaaat tgcctcctct tagagaaaga agggataatc
    15181 tagtggaaat tataaaatat tattttaata ggtactcaag tgacttcaac ctaaataaaa
    15241 ctatttctaa agaagctatg gatgctattg caaattatag attcccaggc aatataagag
    15301 aattacaaaa tataattcaa aaaatcttgc ttacttgtac agataatcat ataacaatag
    15361 atgacttacc aaacattctt acaaaaaata tacatattac caataatgga aataaaacac
    15421 atatatccca aattaataag gttattatat ctgactcaaa atctactaat tataaaaata
    15481 aaaattttga cactttaatt aaagagtatg aaaaaaatat aattttggat gcttatgaaa
    15541 aatttggtag ttcttataaa gtagccaaac accttgaaat ctcacagtct aaagccaata
    15601 ggttaattag aaagtatact aatacttaat tttaaatttg ataaataaaa acttaacact
    15661 atttacatat tgtaaatttg gccatttact gttattatga atatggttga aattatttaa
    15721 ataaatactg ttgacttagt ttcatatatt taccattaaa cttttatcat aaatatcata
    15781 tcataataat atatttaata agtctaataa actatacgag gtgtaaatat gttaatgaat
    15841 acccatttta ttatggcaaa atcaatactt gataatatag atgaaaataa aacattcttt
    15901 ataagcgaaa aaaattttat atatggcaat ataaagcctg atgctttttc taaatataaa
    15961 ttaaaaaaac attatttaga tgagtcattt aatatgattg tttctaaaat aaactatcta
    16021 tgcagtctta atatagatag tctttctaaa tacttttcag taagtagact tagtcaggaa
    16081 ctaggagtca tatgtcattt tttatgtgat tttttctgtg tagctcacag tgaacgttgg
    16141 gagtttaagc atagcttaaa taaacatgtt acttatgaaa gagaacttgg agcatttgca
    16201 aaagatgttg atttatctaa aattaaatgt ggtactataa atagcagttt tgagaaattt
    16261 tttactaaac tttatagtga ttacaaaaag aaaactgatt atatgaatga tttaaaattt
    16321 tcagcttata cttgt
//
LOCUS       DAEMHW010000081        16068 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEMHW010000081 DAEMHW010000000
VERSION     DAEMHW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.081"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(128..1318)
                     /locus_tag="KPK67_002133"
     CDS             complement(128..1318)
                     /locus_tag="KPK67_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG2459099.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESITIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(1578..2009)
                     /gene="dut"
                     /locus_tag="KPK67_002134"
     CDS             complement(1578..2009)
                     /gene="dut"
                     /locus_tag="KPK67_002134"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBG2459100.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            2122..4350
                     /locus_tag="KPK67_002135"
     CDS             2122..4350
                     /locus_tag="KPK67_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBG2459101.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(4461..4946)
                     /locus_tag="KPK67_002136"
     CDS             complement(4461..4946)
                     /locus_tag="KPK67_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2459102.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDL
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            5198..6421
                     /locus_tag="KPK67_002137"
     CDS             5198..6421
                     /locus_tag="KPK67_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG2459103.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAVGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSIEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            6425..9559
                     /locus_tag="KPK67_002138"
     CDS             6425..9559
                     /locus_tag="KPK67_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBG2459104.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            9593..10891
                     /locus_tag="KPK67_002139"
     CDS             9593..10891
                     /locus_tag="KPK67_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBG2459105.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLKYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(11180..11425)
                     /locus_tag="KPK67_002140"
     CDS             complement(11180..11425)
                     /locus_tag="KPK67_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2459106.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(11639..14266)
                     /locus_tag="KPK67_002141"
     CDS             complement(11639..14266)
                     /locus_tag="KPK67_002141"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG2459107.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(14327..15169)
                     /locus_tag="KPK67_002142"
     CDS             complement(14327..15169)
                     /locus_tag="KPK67_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBG2459108.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(15176..15814)
                     /locus_tag="KPK67_002143"
     CDS             complement(15176..15814)
                     /locus_tag="KPK67_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2459109.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
BASE COUNT         5639 a         2539 c         2303 g         5587 t
ORIGIN      
        1 aataaaaagc aggtatcaat aaaatagtaa catgtattat actatacatt actatcaata
       61 ttgtacctgc tttcatatta taagcaataa agaaaaatct atttattagg ttttataata
      121 aaaacaatta taaaactctt cttgcattta catatctact tgaatagtag ctactattta
      181 tattagatat tgttactttt tttgatgaac ctgaagatgc atgtatcata tctccatttc
      241 ctatgtatat tccaacatga cttacagaac cattatttga accttgtgta tcaaagaata
      301 ctaaatctcc acttctaagg tctcctctat ttacatatgt tccatactta ctttggtctc
      361 tagatactct tggtatacta actcctacag attttttcat aacgtactgt gtgaatccag
      421 aacagtcaaa actatttgga ccttctgctc cccatacata tggttttcca agtagtgtct
      481 ttgcaaaact tattacacta tcagctcctt gacttggaga attagtcgaa taagatggaa
      541 ttgaagtatc aacagaacct ttttcagcaa ggtattgact tgacacatat ccagtttgat
      601 tgccataact tacttttgtc catcctccac tttcagaaat acttccaacc tcagtaccat
      661 aacttaatgt agctatttta gaatgacttg tacctggtcc tgttctaacg ttcaatgact
      721 ttgctgttac aacctttgta ccctttacaa ggtcatttga actgttttca gcattttcat
      781 ttgtagagtc atttacacta gttcctaaat atttacttga aacataacct actctaccat
      841 tagaacttat ttttgaccat ccatttgact cagatatgta tccgacttca gtcccatatc
      901 ctagtgttga tattttagaa ctacttgtac ttggtcctgt tctaaaattt aatccttttg
      961 ctgttacaac ttttgtcgac ttaactgaag tatcactact ttcattgcta cttccaaggc
     1021 tatttattgc tacataattt ccatgcacat aacctgtgtt tccattatat ttaactttta
     1081 cccaagaacc ttcttttcct acatactcaa ctttatcacc tttgtaaagt ttccccattg
     1141 attcatggtt tgtacctggt ccttttctaa aatttaccgc attccctgtt attgttatgc
     1201 tttctatact cacactctct acttcagact cattagcata tgctaaattc cccatagtag
     1261 gcattattat cgcagttgcc attatactgg ctgctattct cttttgatta aaattcataa
     1321 ctttccactc atctcctctt ttctactatc tttatttcac ttcaaaatat aaaagaaata
     1381 attgacacta ttaaaatatc aattatttct tttatattaa tattattaca aatttgtaac
     1441 tatttgtcta tagtaatatt tgattaccat aaattaccat gtataaattt taatctttaa
     1501 tagcctgtaa tttagtaata tagccatctt ttaccttttt taaaaatggc tatatttttt
     1561 aacttttttg taacttttta aaatccagtt gagccaaacc caccttttcc tctttcggag
     1621 tcacttaatt cttttacttc ttctatgtta atatcataga taggttttac tatcatttga
     1681 gcaattttca tatgcttttc aaccttaaaa tcatttttac catgatttat taatataatt
     1741 ttaagctctc ctctgtatcc ttcgtcaata gttcctggtg tatttaatac agtaacagaa
     1801 tgctttaagg caagcccact tctaggtctt acctgcgctt ctgtcatagt aggaagctct
     1861 atacatatcc cagttttgat aagtttagtc tctccaggag gtattacaac ctcttctata
     1921 gaatacaaat ccataccagc atcacctttg tgagcaaaat taggtattat agcatcatca
     1981 tttaattttt ttacttttaa attgtacatc ttatatctcc tttcaacttt ctataaatat
     2041 tataacttaa atttcgacct cttgaagttt tttatttttt tgtggtaata taattattag
     2101 tgttttaaag gaggattttt tatgaaactt gtacttgctg agaaaccatc tgtggcaaaa
     2161 actatagcat catttttagg tgcaaagacc agacaagatg gttactttga aggaaatgat
     2221 tatataatta cttatgcagt tggacactta gtatcattat atgatatgaa ggattatgat
     2281 aaagataaat actctggctc ttggaaaatg aacaatttcc catttgttcc tgaggataag
     2341 tttaaattta agattgatag ttctaaaact aaacaattca acactgtaaa aaagcttttg
     2401 aatagaaaag atgtagaata tgtaataaat gccactgaca atgaccgaga aggagaactt
     2461 atagcatttt taatattttt actggcaaaa aataaaaaac cagtaaaaag aatcttggta
     2521 aatgaatgga caccagaaga tattacaaga ggtataaaaa acctaaaaga tgaagatgaa
     2581 atgagaaatc ttcaagctgc tggatatact aggcttatta ctgactggtt gattggaata
     2641 aatttcactt ctgttgctac attgaaatat ggaaatggga aactattaaa cataggtagg
     2701 gtaattcttc caacagttaa attagtctat gatagagata tggaaatact aaactttgtt
     2761 cctaaaactt attatgaaat agaagggcat tttaaagctg aagctggtga atataaaggt
     2821 aaatatgtta agggtaaaga aagtaaattt gatacacttg aagatgcaaa taaaattata
     2881 gcttcaatta catctgaaac tggtaaaata ctagataaaa aagttactat gtcaaaggaa
     2941 tatgcaccaa aactttttag tttgacatca cttcaaggat atataacttc taagtattcc
     3001 aattttacat ctgataaggt tttaaatgtc tgtcaatctc tctatgaggg taatggtaag
     3061 ggtggataca taacttaccc tagaactgat tcaattttct tagaagaaag tttggcttca
     3121 aaagcttctc aaactttaga taaattaaag gttggtcttg aatatgaaaa taaaattaaa
     3181 ttttctaaaa ctaaaagagt ttttgattct tctaaagtag actcccatag tgctataatt
     3241 ccaacttata taattccaaa aagtttatct aaggatgaac aattagttta tgatgccata
     3301 aaagatagat ttgtagcaaa ctttatgcct cctgctgaat atgagaatac agaaattaag
     3361 actgaagtag ataattgtac tttcttaaca aaaggtaagg tacttaaatc aaaaggatat
     3421 cttgaagtgt ataataagga agaaaaaaac gatttacttc cacttgtaaa taaagatgat
     3481 gttgtagatg tgttagaaat aaaaccatta actaagcaaa ctactccacc taaaccatat
     3541 actgaggaca cacttttaaa ggctatgaaa aattgtggta aaaatgtgcc tgaagaagat
     3601 acaacagtgc tatctggata ttcgattgga acatcagcca cacgtgctga tgtgttaaag
     3661 aagataagtc aagtgggcta tgtaaagaaa aaagggaaat cttattctat aacagacctt
     3721 gggaaaaatt tagttgaaat ttttcctgtt aaagacttat ttgatgtaga ttatactgga
     3781 aaacttgaaa aaagtttaag cgatattcaa aaaggaaagt atacaagaaa agaatatctt
     3841 actaatatca ttagttttat atatcaaaat gtcaatatta taaaacaaga tgcttcaaaa
     3901 aatataaaca cagaagcata tactttcgat tcaaaaacta aaaaatttac taaaacaaaa
     3961 gaaaaaaaac agaaaaaaga tgtaactaat actgaaaaag aagttgctat atcaaaggat
     4021 tctaataaca aaaaagaagt taaaaatggt gatgaaaatc aatctcttgg caaatgcccc
     4081 atttgtcaag gtgatgtact agaatttgac aaaggctttg cttgtaaaaa tcataaagaa
     4141 tgtaaatttg ttatttggaa aaatgataag tatttaagtc tttataaaaa gaaagttaac
     4201 aaaacaatgg tcaaaaatat tttaaaaaaa ggtgaaacta aagtaaaatc tttaactgct
     4261 aaaaacggta ataaatttga tgctacatta aaatatgata aaaatccaga tactggctat
     4321 ttcaattgga aaatagagtt tgacaactaa atattcataa gaaaagaaat agcagtagta
     4381 taataatcta tattatacta ctgctatttc ttttcttatg aagttttaac ttttttaata
     4441 gtgattttta caatcacact ctaacctaat ttctttacta tttctgatag ctttaatgtg
     4501 tgttcatcta ataaagttag ttcctcttca gaaagattag ataatttttt tactagagat
     4561 tctttgagaa tagcttttac tgcatcaaat aatttaaatg ctttatccgt taattcaacc
     4621 ctaagaatcc ttctatcttt taggtcagga aatttattga ctaaaccatg ttccactaat
     4681 ctgtcaataa tcggagtcat atttgatttt aaaattccta aattagaagc tatttgagaa
     4741 attgatgatg aatttgatgt cataagataa agaattacct tagcatgtga tggagccatt
     4801 ggacattctt caaaataagc tttcattata tcagagttat tttgaaactt ttttaacaaa
     4861 tcgctttctt taaatatatt attttcaagc acaaataaaa aatctaaaaa agagtccgct
     4921 attttaagta tattgttgtt atccatattt gtaacatccc ttcaatagat aatataataa
     4981 aacattgttg tttaatatag tataaattat aaactattaa taaatttaaa acaagaaaaa
     5041 gttattgact cataataatt ataaatttat aatcaaatta gagaattata aattgccaat
     5101 aaagggaagt tttttaatta gtctaaaact aaacttaaat tataagacac tttaaattct
     5161 acaacaagct agtgtgcaat atataatagg aggagatatg aaaagatatt taatagtttt
     5221 cttaatgtgt agttcactgt ttttagttgg atgtggaaaa acagaagaat ctgaacctga
     5281 gaaaccaata gcagtttctg ttcaaaaagc agtaggtgga gaaattgaaa acacaaattc
     5341 tttctcagga actacaaagg ttaaagatga aacagctgtt actgcacaga ctgttggcac
     5401 agttcaagaa gtgtatgtaa aacttgggca aaatgtaaga aaaggagatg aattattgag
     5461 tataagcagt ccagagctag aaaatagtgt aaagcaaagt aaagcctcac tagatttagc
     5521 aaaagcaagt tattcaagtg ccactggtgg ttcattagag gcacaagtaa atcaggcaaa
     5581 aacagctctt gacaatgcaa agattcaata tgaggagtct caaaggaatt atgacaataa
     5641 taagattctt tatgaacagg aagtcattag tttagaccaa tttaaaaaaa tagagttttc
     5701 tttagagcaa acaaaacaac agttagattc tgctcaaaga gcatacgata cagcaacttc
     5761 aaaaagtata cctcaagcta aagcacttgc taaaaaacag cttgaccaag ctcaagtatc
     5821 atataattta gcaatgagta atttagacaa attaacatta acatctccag tagatggtac
     5881 aataacagct aaaaactttg attcaaagga aatgataaca caatcacaac cagcttttat
     5941 tatttctaat cctaatatac tagaagttga tttaaatgta gcagaatcag atataggtaa
     6001 atttaagaaa gatggaaatg tagatgttat aattgaagac caaaggattt taggaaaaat
     6061 agattatgta ccttcagtag ttgaccctca gacatcttta tatcctgtta aagtacttgt
     6121 aaataataca aacaacaaat ttaaggcagg tatgtctgca caagtaaact taagtattga
     6181 aaaagaaaat ggtgctgtta cagtgcctaa aaaagcaata tttgaagaaa atggtaaaaa
     6241 atatgtatac atagcaacaa aagacaatat agcaaagaaa cacttggttc aaactggaat
     6301 tgtaacagag gataaaatag aaattaaaag tggtgtaagt gataaagata cagtgattat
     6361 tggagggata agtcttattt ctgacggaac taagatattt cctgtagaaa aggagaagta
     6421 gactatgaat ttaacacaga cttccgttaa acgtcctctc acaatcataa tggtcttttt
     6481 agtattaata gtttttggtg gtattggata taaaaaaatg tcaataaatc taatgcctga
     6541 tatagaaata ccagttgtaa tggtcatgac tacttggaca ggtgctggcc ctcaagatgt
     6601 tgatgaacaa gttagtcaaa aagttgatga aagtttatct gccgtttcaa atgtaaaatc
     6661 tactatatca agttcacaag aaagtgtttc tatggtagtt gctcagtttg aatttggaac
     6721 taatttggat gaaataatga atgatgttag gtctaaagtt gatgcattac agaccagcct
     6781 cccagatgat gcagctaaac caacagtttt aaagctggat atgaatgcac aggctatagg
     6841 tcaacttgtc atatctggag gaaatgaaaa tagttcacaa gccttaagaa aatacgctga
     6901 agatgtaatt cagcctaaaa tagaatccat agatggtgtt acttcagcag accttaaagg
     6961 tggagaaaaa gcacaggtta atgtaatagc tgacccagct gttctttcaa attatggagt
     7021 tagtttatcc actataaaag gtgtcttatc ctctagtaat aaaacattcc cttatggttc
     7081 aattactcaa ggtgaggata aaatagtttt aagagcaatt gataaattag aatccctaga
     7141 tgatataaaa cagattcaaa ttcctgtaaa aggaggaaat acagttagat tagataatct
     7201 ttgtagtgta gattatggat atgcagacaa agatagtatt tatagataca atggaaaaga
     7261 cagtttggta atagatattt ctaaacaaca agatgccaac actgtaaaag ttatgcagag
     7321 tgcatacaaa tacgtagatg aattgaataa ggaaaatcct caatttaaaa ttaaaattgc
     7381 taatgataca agttcataca taagtgaatc aataaacagt gtaatgagca acttgcttat
     7441 aagtgctgtt atcgcattta ttgtaatatt tgctttccta aaaagtgtaa gagcttcact
     7501 agttgttgca gttgcaatac caatttctat aataggtgct atagcaattc tctactttac
     7561 tggtgaaagt ttaaatctta ttactgcaag ttcattggta atttcagttg gtatggttgt
     7621 tgataatgct acagttgtaa ttgaaaatat atttaaatat aggaaaagtg gcagattgag
     7681 tcttgatgat tgtgctattg agggaacaag aactgtaaca aatgcaatac ttgcatcaac
     7741 attgaccaca atagcaatat tcttacctat catgtttaca gaaggtatag ctaaaataac
     7801 ttttggggca ttaggtaaaa cactaatagc ttcactagca ttctccttta taggtgcaat
     7861 tacactcgtt cctagtgtat ttgcaaaatt aagcagagga aaaaatagtc aaaagatgca
     7921 agaaaaagat agtcctatat ttgataaggt aagtgaagca tataaaaaat tattaagtgt
     7981 atgtttgaga catcaacgct tagttgtaat atcaagttta cttatttttg tagcttcctt
     8041 atttggaatg ggatttatag gtatggactt tatgcctgca gcagacaagg gtgaattatc
     8101 tatttctata gaacttccaa aaggtcttag cctagaatcc aatgattact atgtatctat
     8161 gactgagaaa aaagtctcag atattccaga aattaaaact ttaataacaa ctttgacatc
     8221 agattcaaat aatagtaaag caagtatagc aattgaatta gaatctcaaa aaaatagaaa
     8281 aagaactact gatgaaatag aaaaagagat aattgataga gtggctactg taccagattg
     8341 taaaataaat gtaagtcaaa acagtagtgt tatgggtgga gctggaggag cagacttcac
     8401 attagaaatg aaaggtcctg atttagatgt attaaaaata ctgggaaaag aagctgaaaa
     8461 agaatttagc tctataaaag gatttagaaa tgttgaaact tctcttacag atacaacaca
     8521 agaagctcaa tttactatag ataaacataa ggctcaagaa tatggtgtaa atactgctga
     8581 aattgcaggc atgttacgta cagcaataaa tggtgactcc gtaaccactg ctactattga
     8641 tgattacaaa gtggatgtaa acctaaaatt taaagcagat actattgata gcttagaaga
     8701 tataaagcaa ctaaaagtaa actctgcaac tggaaaagaa gttcctcttg gtgctttttc
     8761 agatataaaa atggcagatg gtctacaatc tatatctagg actgatggag atttttctgt
     8821 aaatataact gctacacttg acaatttaga tgctggtact gctacaaaac tagcactaga
     8881 aaaaacgaat aaaatggtaa ttcctaaaga ttactctata ggtgttggtg gtgaaacaga
     8941 aatgatgaat gaatctatgg caagtcttgg attttcaatg gtcatagcta tagcattagt
     9001 ttatatggta atggtagctc agtttgaatc atttagtaaa ccatttataa tcatgttctg
     9061 tgttccattt gcattcgttg gagttgtgct ttcgcttcta gttagtggtc aaagtttaag
     9121 tactgtcggt atgctaggta taatcttact tatcggtatt gttgttaata atggtatagt
     9181 tcttattgac tacatagagc aacttagaaa aacaaatcgt aacaatgatt taattggttt
     9241 ggtttcaatt ggttgtgcag caagattaag accagtctta atgactacag caacaacaat
     9301 tttaggtatg ttgccaacag cactatctct tggtgaaggt ggagaaacta tgcaaccact
     9361 agccatagtt ataatgggtg gtctaactgt atctacactt ataactttgg tgcttatacc
     9421 tactatatac ttaatatttg ataaatggga aaataagttt tcagacaaaa tgcctaaaat
     9481 atttggtaag ataacagata aaataggaag tataaaacgt aaaataataa agaaaccaaa
     9541 attgaaagct aaagtttaaa tagaatggag ggtgattgta ttgaataaaa aaatgagcaa
     9601 aatagttgca attggctgtg gtgttggtat aataactgcc aatttaatcc cagtttacgc
     9661 agataataat acttcttcta taaacaaagt tgataattct attttaagtg actcaaataa
     9721 aagaacatct gaaaaggaaa ttttgactgc tagagaagct gttagtgctg cacttaaaaa
     9781 tagtgaaaaa ttaaaaatga aatcagaaga aataaaaatg ttaaaagaaa agttagaagt
     9841 acaagatgag tttgatagtt ttactggaag tgacaactcg tttccatacg accaaattgg
     9901 acttcttaaa aatcaaagtg aacaggctaa aggctttatg gaggaccaaa tagcaaatga
     9961 tattactaat aaatttaacg atttagtttc aagagaaaat gagctagata agataaaaaa
    10021 caatcttgaa ataaaaacta aagaaattaa agatatgaaa ttaaaaaaag atttagggtt
    10081 agtaacatct ttagaaactg aatcagctga acttgaactt caaacactac aaaatactca
    10141 aaaagctaaa cttcaagaac taaaaaacaa tcaagactat tttaagttat taactaatat
    10201 agatttaaat aattatcaat tggataaaga atatagattt gaatctttta gagtaggtgg
    10261 ctccgttgat tcatatatgg aaggtaaggt taatgaatat ttaaaatacg accaattgat
    10321 tttagaacgt acagaagaaa gctttaatga taaagatgag aacaaagcag atttaccaga
    10381 tagacctaac cttacaagac cagttgctcc tacaaaacca gagcaaggta acctaactga
    10441 tgatgaatac aaggttctta tggataagta tgaaaaagat tatgatagat ataaaaaaga
    10501 agttgaagca tacaatcttg aaagacagac ttatgctgct ggccttacta cttatgcaaa
    10561 ttatcttcaa caaaaattta atactgaaaa tggtctagta acattagaag acagtaaaaa
    10621 agctcttaaa aaagggttaa ttgatagcta tgctcaatta cttgctttag aagatacaat
    10681 tcagattact aaaaaacaac ttgatttatc agaaaaacaa ttaaaaaata ctaaattaaa
    10741 atatgattta ggattaatta ctctaactga ttataaaaaa caagtggtaa gtaatgaaga
    10801 tgctaaaaat agctatgaca ctcttattgt gaattataac tcactaaaaa atggtataga
    10861 aaaaccttgg atacttaata ctggaaaata attctggtaa atagaaagat tactttaaca
    10921 tacgtttgat taaaaaatag ttatgcacac tcgtggcttt agtcatggaa gattcagaag
    10981 gcaaataata gaaatagatt agtaaaaata tatttactta taaaactgta tacttttata
    11041 gaggctatct caaaatagag ataagcctct tttttattgt ttaaattata aaaatatatt
    11101 tgattccata caaaaagagt gccttatgaa ctaaaaagtt cattttgaga caccctcaaa
    11161 agcttaactc taaatttaat tattttttat caaatgtctc atctatttta catctcacca
    11221 tgttaacaaa ttcagaaata ttatatcctc taagtttgca tccacattta ggacaataat
    11281 ccagtttcct agataccaaa gccctacaac taggttctgg acatcttatt tttagactct
    11341 catctgctac tttaaatcca catcgtgggc atatactacc tggacgtgat gatacttcct
    11401 ttccacattt aggacacttt gtcaacttta tcccctccag taaactcaat ttaattgata
    11461 gttgaaattt ctctctacaa taatatatgc aatataatta aatacgtttc acttacaact
    11521 atataagtta aatttttata ataaaaatcc cctatattca atttacaaat acttaatttg
    11581 atacattgaa caatagggga ttttattttt atttagttac atattttgca gtctattatt
    11641 atctaggatt ttttatagat gcttgagctg ctgcaagtct agcaattggc actctaaatg
    11701 gtgaacaaga tacatagtta agtcctacac tataacagaa ctctactgaa gatggttctc
    11761 ctccatgctc tccacatatt cctagtttta attctggttt aacttctcta cctaatttag
    11821 ctcccatctt aactaactta ccaacaccat tttggtctag tacttggaat gggtcttttt
    11881 ctaatatttc ttcatctaca tattgaccta agaatttacc agcatcatct cttgaatatc
    11941 caaatgccat ttgagttaag tcattagtac caaaactgaa gaagtctgct tcttgtgcta
    12001 tttcatcagc agttaaacaa gctctaggta tttctatcat agtacctaca gtataaggaa
    12061 cttctatacc ttcttctttt attatagcgt caacagtttc tactatcatt tttcttatta
    12121 tttttaactc atttacttct ccaactaatg gaaccattat ctctggagaa actttaacac
    12181 ctgctttgat tgcttctata gcaccttgta ttatagcttt agattgcatt acacatattt
    12241 caggataagt tattgcaagt ctacaacctc tgtgtccaag cattgggtta aactcatcta
    12301 aatcaactat tctcttctta atatcactta cttttattcc catagatttt gaaagttctt
    12361 ctatagtctc atcatcatga ggcaaaaatt catgtagtgg tgggtctagt aatcttatat
    12421 taactggttt tccatccata actttaaata actctacaaa atcctctctt tgcattggta
    12481 atattttttc aagagctttt tctctttgtt ctactgtatt agataatatc atttctctaa
    12541 ctgctggcaa tctagcttca tcaaagaaca tatgctcagt tctacataga cctatacctt
    12601 ctgcaccaaa ctctatagct gcacgagcat ctctaggatt atctgcatta gttctaacca
    12661 tcatacattt gtgtttgtct acccagttca ttaatttttc aaagtttcca gtcaaagata
    12721 cttctgtttt cttaacatct cctaggtaca catttcctgt agaaccatct atagaaatgt
    12781 attcaccttc ttttataact acatcatcaa gtgctcttat ttctttatta aactcatcaa
    12841 cttttatttc tccacatcca gcaacacaac atttacccat acctctagca acaactgctg
    12901 catgtgatgt cattccacct ctagctgtaa ggattccttc tgctttaacc attccttcta
    12961 tatcttctgg agatgtctct agtctaacta aaactacttt ttctcctttt tcatttgctt
    13021 ttactacatc atcagcattg aaataaactt taccacttgc tgcacctggt gatgctggta
    13081 aaccttttgc tataacctta gcttctttta atgccttatc ttcaaatttt ggatgtaata
    13141 attggtctaa ttgattaggc tcaattctca tgatagcttc tttttcatca attattccag
    13201 cttcaactaa atcaaccgct acatttattg ctgctgctgc tgttctctta ccatttctag
    13261 tttgtagtat atataatctt tcattttcta tagtaaactc tatatcttgc atatctttat
    13321 aatgaccttc aagtatatga gtaattttta caaattcatc gaatacagct ggcattacat
    13381 ctgcaagagt tgatatattt tgaggagttc ttatacctgc aacaacgtct tcaccttgtg
    13441 cattcattaa aaattcacca aataatttat tttctcctgt agctggattt ctagtaaatg
    13501 caacacctgt accactagtt tctcccatat taccaaatac cattgattga atatttactg
    13561 ctgttcctaa gttatgagct atatcattca atttacggta tacgattgca cgaggattat
    13621 tccatgatct aaatacagcc tctattgcaa gcattaattg ctcttttggg tcttgtggga
    13681 aatcattttt tatttctttt ttataaattg ctttaaattc ttttactatt tcttttaaat
    13741 catcagttgt aagttctgta tcaactgtgt atccctttgc ttcttttact ctgtctaata
    13801 cgttttcaaa tttatatttt ggaacttcca tagcaacatc agaaaacatt tgtatgaatc
    13861 ttctgtaact atcatatgca aatctttcat tttgtgtagc ctctgctaga ccaacaacac
    13921 tattgtcatt cagacctagg ttaagaattg tatccatcat tcctggcata gagaatactg
    13981 caccagaacg aactgatact aataatggat tcttattgca tcctaatgtt ttatttaagt
    14041 ctttttctaa agtagctaaa tgctcttcta tttcttttat tatctctgct cttatacttt
    14101 cattgttaac ataataatca ttacacgctt ctgttgttat agtgaatcca ggagggacag
    14161 gtaagcctat cttagtcatt tctgctaaat tggcaccttt acctcctagt aaagatttca
    14221 tgtctttgct tccttcacca aaactataaa catacttagt ttccattgtt aaatccccca
    14281 ttaatctttt tattttaatt tatatcattt ttattaatag acattattaa atttctattt
    14341 ctttgtatat ctttagacca ttttctttca ttatgtctaa aattatacct gctgtttctt
    14401 ctattgcctt atttgaaacg tttattactg ggcatccaat tcttttcatt atctcttctg
    14461 aataatctaa ctcctgaagt attctttcta aattagcgta gtttgcttta ctggacaatc
    14521 ctaaagcttt taatctttgg gttcttatct cattcagctt ttcaggtgaa ttagtaagac
    14581 ctattatttt tttagtatct atctcaaaca cttctttagg aattggtatt tctggaacta
    14641 atggaacatt agctactttt atatttttat tagctaaata catactcaaa ggagttttag
    14701 atgttcttga tattcccact aaaattatgt ctgcttgaag aacacctcta gggtcttttc
    14761 catcatcata tttcactgca aattctatag cttcaactct cttaaagtaa gattcatcta
    14821 attttctaat tataccagct tctcttttag gttttcttcc tgttctcttg cttatttctc
    14881 ttagtatatc tgtcattaaa tctatacaac taagaccttc tctttcgcaa tatctttcaa
    14941 caatactgca taattcttca tcaactaatg tatatacaat taaagcgttt tcagcttttg
    15001 cattctccaa agtttcaagt agtactcctt tattaatcat gtatggaaat ctttttattt
    15061 cataagtttc attcacataa aactgagaca tacatgcctt tgcaacttgt tgtgcagtct
    15121 cccctactga atctgatact gcatatataa ttaggttttt caccagcata cctccttaat
    15181 tgtcaactat ttctaagaat agcttagtta tgttggtttt tgatattctc ccaacaactc
    15241 tcatatgatt tttgtcttct cccttatatt caacaatcgg aatcgaatct acttcatgtt
    15301 ctattatttt tcttgaagct aataaaacac ttgctccttt agttaaagtg actacatttg
    15361 gagttcttgt cattatcata cctattggca ttttatttat atcagaacct cctatagttg
    15421 cttttagcaa gtcttttctg gaaacaactc cacataactc ttcattctca tctataataa
    15481 ctatactccc tacatcagat aaaaacatag tgactattac atcatatata ctttcatcct
    15541 tcttagctag tacaggcatg ctcattatat cctctactgt tttttctttt acacttttac
    15601 caattaaatt tatttcacta actcctgaat aaaaatatcc aacttttggt cttgcgtcta
    15661 atattcctgt cattgttaat atagctaaat cagatctaag tgtagcacga gtcacattca
    15721 agctggaggc aatactttca ctcgttattg gttcattttc cttaactata tctataattt
    15781 ttagttgtct ttcattaagc tgaataataa tcaccttctt tcattgtgct atacttttta
    15841 tcttttcgca tcttatatag tatattatat aactatatat accattctgt aaacagattt
    15901 tttattgaat acgatatatt ttttactaag ttctctcgct taaagatatt tattatatat
    15961 taatggggga tatttcttta attaatagaa ctatattagt attacagtta atattataat
    16021 acataattgg tcattttaat tcattttata ttaaaatttt taataata
//
LOCUS       DAEMHW010000082        15584 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEMHW010000082 DAEMHW010000000
VERSION     DAEMHW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.082"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..3138)
                     /locus_tag="KPK67_002144"
     CDS             complement(<1..3138)
                     /locus_tag="KPK67_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2459110.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVL"
     gene            3419..5596
                     /locus_tag="KPK67_002145"
     CDS             3419..5596
                     /locus_tag="KPK67_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2459111.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASIRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADVNSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            5610..6032
                     /locus_tag="KPK67_002146"
     CDS             5610..6032
                     /locus_tag="KPK67_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2459112.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            6206..6781
                     /locus_tag="KPK67_002147"
     CDS             6206..6781
                     /locus_tag="KPK67_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2459113.1"
                     /translation="MVKVLAFMGSPRKKGNVDTILDEIIKGVNDNNVEVKKYYLNDMN
                     IKGCQGCLYCRKVPTCAFKDDMIDIYEDIKSAEYIIIGSPVYICQVSAQTKLLLDRLY
                     PLTEINKSKHTPRFGQKKLIMVYTQAAPISFLFKKYFKYTAKHLKGMGLNHYKTIVVT
                     KAFERNSTKKNFKVLKKAYKLGMKIAIDING"
     gene            complement(6906..7622)
                     /locus_tag="KPK67_002148"
     CDS             complement(6906..7622)
                     /locus_tag="KPK67_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2459114.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFQNKLDI"
     gene            7787..8512
                     /locus_tag="KPK67_002149"
     CDS             7787..8512
                     /locus_tag="KPK67_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2459115.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            8572..10164
                     /gene="hcp"
                     /locus_tag="KPK67_002150"
                     /gene_synonym="priS"
     CDS             8572..10164
                     /gene="hcp"
                     /locus_tag="KPK67_002150"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBG2459116.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            10536..11441
                     /locus_tag="KPK67_002151"
     CDS             10536..11441
                     /locus_tag="KPK67_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG2459117.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            11565..12518
                     /gene="msrA"
                     /locus_tag="KPK67_002152"
     CDS             11565..12518
                     /gene="msrA"
                     /locus_tag="KPK67_002152"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBG2459118.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            12670..13800
                     /locus_tag="KPK67_002153"
     CDS             12670..13800
                     /locus_tag="KPK67_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2459119.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(13917..14885)
                     /locus_tag="KPK67_002154"
     CDS             complement(13917..14885)
                     /locus_tag="KPK67_002154"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBG2459120.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            15220..15408
                     /locus_tag="KPK67_002155"
     CDS             15220..15408
                     /locus_tag="KPK67_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459121.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
BASE COUNT         5898 a         1828 c         2437 g         5421 t
ORIGIN      
        1 taatacacct tcattagaaa gttctccatt tataaattga tttataagct cttttttact
       61 ttcttgtaat tttattattt tttcttctat agtcccttta gcaatcaatt ttataacttg
      121 tactgaattt ttttgaccaa atctatgagc tctatcacta gcttgatttt ctactgatgg
      181 attccaccat gggtcaaagt gaattaccat gtcagcagat gtaagattta aaccagttcc
      241 gcctgctttt aaagatatta aaaaaacttt tttatccata ctattattga actcatctac
      301 aagttcaagt ctttctttgg cattagtttt accatctata taatggtatt taattttata
      361 cttatcaagt tccttactta tgttcttaag cacagaagta aattgtgaaa acaataatat
      421 tttgtggttt tcgtttatac tatccctaag tatttctaaa catgtttcaa tttttgaact
      481 ttttttattg taatctttta ctactatact tgggtctaaa caaagttgtc ttagtttagt
      541 taaataagaa aatataacta ttttatcttt ctttaaattt ttatctttca ttttatcttg
      601 tatatctttt gaatatacac tatatatctt tttctgctct ttattcaatt caacaaaaaa
      661 gtttttttca atcttatctg gaagttcttt cataacttgt tttttacttc ttctaagaat
      721 aaaaggtttt atcaattttt tcaggttttt aacattatct tctttattta taaacaattc
      781 ctgaaacttt gctttactat atagatagcc aggcattata aaatcaaata tagaccataa
      841 ttctagtaga ttattttcca taggtgtacc agtgagtgca aatttatttt ctgcatttat
      901 actttttaca gactcagtta ctaaagctac tggatttttt atattttgag cttcatctaa
      961 tatacaataa tcaaacttta tttcactata tttttctaaa tcatttctaa gagtaccata
     1021 tgttgtaagt attacatcaa aattttctaa ttccataaaa catttttctc tatctctttt
     1081 atttccatgt atcaataaaa cttttatatc tggagcaaac ttttcaaatt catttttcca
     1141 attataaatg agtgatgtcg gtgtaacaat aagacttttt ttattagata atgataataa
     1201 aaatgcaata gtctgtattg ttttaccaag acccatttca tcagctaata taccaccaaa
     1261 tttataataa tctaaaactt taaaccaatt gaggccattt atttgatagt cacgaaggtt
     1321 tgcatttaaa tttgcgggaa tttctaaatt tatgctatca aaatttctaa atttttcaca
     1381 tatactgtct agttcttttt taccatctat aaactttaaa tttttactac gtattacatc
     1441 atttatatat agagctttat ttttatgaat tttattattt tttgatttat ccattatatc
     1501 tagattttca actaattcaa aaaaatcttt tgtttctctt tctgataaat ctataaagtt
     1561 tccatcttta agtttataaa aagtacgttt ttcttgaaaa gctttcaaaa tatccttgta
     1621 ctcttttgga tttatatcag atatattaaa attaaattct aggtaattct ggttagtctg
     1681 attgattgaa gcttgaattg atgaagcgtt atatattttt ttcaatttaa acttctcaga
     1741 ataatatata tctccaaatt cctttagaga ttctaattgt aaatctaaaa atgtaaatag
     1801 gtcatggtca gttcctttaa acacataatg attattttcc tttgtaaatt gtgaagaagt
     1861 aagtttattt tcaatagtag cttccttttg tatgttgggg agcacaaatt ttttatcttc
     1921 atcatcatat atatacttta catcacaaca tatgttgcca tcaatcatat caaagtagaa
     1981 ctcagattta aaatcttttg ttatattatt ttttatgtta tcatctaaat gaacttcttg
     2041 agatatagat tttaatctag gaactagcaa tcctaaaaca tccttagaat cctctttatc
     2101 aaattttaac tctttagtct ttgataatat ttgatatatt tttgaataat catgagcttt
     2161 tttatgtgat aataaataga tattgccatc atataaaagt acatcatttt ttttagtaag
     2221 tggaacaggt agtgaatctt tagaatataa gactatttta tcttctatat ttttaaaatc
     2281 aaaccttaca ggtagagtat catttaatat ctttggatga tattcctctt tagaaaaaat
     2341 aagggtaaaa tccttgtact taatagcatt taaaaattgt tttaaagagc ttgcatttat
     2401 ctccataaat ttttttgaat gtgatgaaga aaagttgtat gaaagatttg tcccatattc
     2461 ttctataata tctactaact tctcatcttc atcagcaaaa taatgaatgt tggggttgta
     2521 agtaaatttc ttcccaaaat ctaattcata tccattaagt tttgaatatg caaattcagt
     2581 aatgtttttt aaaatgtaca ttttatcaat tcctatttta aattcagcaa ttgctttatt
     2641 tgaatccaca tttatcaata catctaaatt aaccctttct cttttagtat tgtctaacaa
     2701 agaaagtaat tgcttgctta aatctaagct ggttttagaa tttgtattag aaagcttatc
     2761 cttatctgca cctaatatat ctattagaat aggaggaaca tcttttttat tttttatttg
     2821 ttttaagctt tttatcacaa tagcaactat atgcttacat ataaaaatac ttttgccctt
     2881 tgattgacag tcagcgcaac tacaatcatt ttcaattatt ttaaagtgct ttatatctat
     2941 agttattgaa ttaaaataaa agctataatt atcttcagaa ttgactccac cacgaaatgt
     3001 caacaggtta ttttgtaggt catcaatatc tatgtctata taatcaacta attgattagt
     3061 ataatatttt acacctcttg accatcttga tggttctgta tttcttctta ttagtttata
     3121 tatcatatct attttcatat tattcacctt acctcactgt atatattata atctaaatgc
     3181 attcaattta cactctaaat ttaagactaa aattaaggca gatatgaata gcttttatct
     3241 atcacaatat atgtgtaaat aacaatttac tattagattt ttattgaatt atcttataaa
     3301 ctatgaatga aaaataatta tataaataga ttattaataa atataaaatc ttattattaa
     3361 aaatattctt agaaattaca ctatataaaa tataaagtat gatatttgaa taaaaagaat
     3421 gtataataag tataataatt tgtatttatt attgggaaag gaaaatgtta atatggatag
     3481 ttatagacaa cttaaagcta aaataccctg taaggaaaca ggtatagaaa taaagaggac
     3541 tttatgtgat atttgttctc caggagtaca ttgtggaatt gatgcatatg ttaaagatgg
     3601 taaagtaata aaaatagaag gtacaaaaga acatccaatg aataaagggt tgttatgtac
     3661 caaaggtctt tgcaatagag aatatatcta tagagaagat agaataaaaa cacctctaaa
     3721 aagaattgga aaaagaggtg aaggaaaatt taaaccaata tcatgggatg aagcttatag
     3781 agaaatagca gaaaaattaa atagagtaaa atctgagtat ggagcaaagc atgtagcttt
     3841 ttttacgggt tatggaaagt ggtatagacc ttttttacaa aggtttgcat cttcatttgg
     3901 ttcacctaat tatggtacag aatctagttc atgttcaaca tcagcacaga tggctggaaa
     3961 tgttgctact ggaagaaact ttacaatgca agatatggag aatgcaaaaa cttatttagg
     4021 gtggggattt aatccatact actctaaatt tttaaatagt gtgaatctaa taaaattaaa
     4081 agaaaaaggt ctaaaattta taattgtaga ccctagaata acaccgacaa ctcaaaaact
     4141 agcagattta catttaagaa ttaaacctgg aactgatgga gctttagctc ttggaatggc
     4201 gaatctattt atacaaaagg atttaataga tagagaatac atagaaaagc atgtacatgg
     4261 atttaatgaa tataaagaat atgtaaaatt atttgattta gatactgtaa gtagaattac
     4321 aggagtttct aaagataata tagtaaaagc tgttgagatg ttgtcacaag gtccattttg
     4381 tataaatgaa agtgcagctc caatagttca tcatcaaaat ggatttcaaa actatagagc
     4441 aatgatgtct ttatctgcta taacaggtaa ttatgataga ataggtggaa acataccaaa
     4501 acaagattac tttgctggta agataggtgg attccaaaca ctagaaaaag agtttgtaaa
     4561 agaagttcta cctaaaaata taaatgaaag aattggtgga gaaagatttc ctctatggaa
     4621 tgaaatggtg actcaatttc aagctatgga tttacctagg caaatagaag aaaagacacc
     4681 atatgaaata agagctttgt tttgtcatgg gataaatgct agaatgtttc ctaattcagg
     4741 gagacttttt aaagcgttga atagtgtaga tttttttgtg aatatagatt tatttatgac
     4801 agatacatca aaatatgcag atatactttt acctgcatgt acatcatttg agagagaaga
     4861 atgtaagagt tatccaggag ggtatattac atataccaaa aaagttattg ataaattata
     4921 tgattcaaaa tcagatgtag aaatactttc agatttagca aatgctatga atatagatga
     4981 tgatttatta aaagcaggtt atgaagcaag tataagacat atgtttaaaa atacatgtgt
     5041 agatgtagat aaacttaaag aaagtgattt accattaaaa tgtaaagatt ttaagccata
     5101 tatagtaggt tcatatacaa gagaagggta tgatactagc acttctaggt ttgaattaaa
     5161 atctacaatt attgaaaaat ataaagattt tggactggat gcactaccaa catatagagg
     5221 atttaatgat aatatagatg atgaatatac tcatttattg acatcaggag caagaatttc
     5281 aaatgcacta cattcaagac ttcatgacat caaactttca agaatattaa aaaatgaagc
     5341 tagttgtgat atttctagct tagatgcaaa agaattgggt gttatcagtg gagattatat
     5401 aaaaataact actaagatag gaagtttgga tgtaaaagtc aatgttactg atagagtctt
     5461 atataagaat atacatatgt atcatggata tagagaagct gatgtaaata gtattatttg
     5521 tgataatttt gacccttata gtggattccc tgcgtataga agttgtaagt gtaagattga
     5581 aaaaatgaat agataaatgg agagatataa tgaaaagttt atttaaattt aataaagaat
     5641 tatgtacagc atgtggagca tgtttgcttg catgtataga ccaaaatgat aataatatag
     5701 acaatggtga actttttaga catgtattta caatagagga aggtgaaaat atagaatatt
     5761 tttcattagc atgtatacat tgtaatgagc caaaatgttt aggtgtatgt cctaaaaatt
     5821 gttttaaaaa agaagaagga ttcgttgtat tagacaatca aaactgtata gggtgtaaac
     5881 tttgtgaaaa agcttgtgaa tatggagcct taatttatgg aaatgatggt aaggcaaata
     5941 agtgtaatgg ttgtattgag agatataagt gtggtatgga ttcaccttgt gtaatggtat
     6001 gtcctacagg agcattaaaa tttaataaat aattgtatta aattgctata ttacttttaa
     6061 ataattgtgt taagaattat tttttgctat acttttgtaa tataaaatgt aaataaaaca
     6121 aatacaaagt atataaaata tatttttaga ataaagtgtg ctttagatta aatttagaaa
     6181 aataaattag acaggagaac ttaatatggt aaaagtatta gcctttatgg gcagtccaag
     6241 aaaaaaaggg aatgtagaca caatacttga tgagataata aaaggtgtaa atgataataa
     6301 tgttgaagtt aaaaaatatt atctaaatga tatgaatatt aaaggatgtc aaggctgtct
     6361 atactgtaga aaagttccta cttgtgcatt taaagacgat atgattgaca tatatgaaga
     6421 tataaaaagt gctgaatata taataattgg ttcgccagta tatatatgtc aggtatcagc
     6481 tcaaacaaag ctacttttag atagattgta tcctttaaca gagataaata agtctaaaca
     6541 tacacctagg tttggtcaaa aaaagttaat catggtctat actcaagcag ctcctatttc
     6601 gtttttattt aaaaaatatt ttaagtatac tgcaaaacat ttaaagggca tgggattaaa
     6661 tcactacaaa actatagttg taaccaaagc atttgaaaga aattccacta aaaaaaattt
     6721 taaggtattg aaaaaagcct ataaacttgg aatgaaaata gccattgata tcaatggata
     6781 gataaaagtt taatttatag tatagctaaa ggagcattaa ttattaatac tccttttttg
     6841 tataaaggtt tctagaaaat aactaataaa aaacaatatt atttgaatac agatattata
     6901 caagcctaga tatctaactt gttttgaaaa taaaagattt caaatttatt tttgttaaaa
     6961 gataacaagc cttcatctcg catcttgcca agctctcttg acatagaact tctgtcaaca
     7021 cataaaaaat cagccatttc atttcttgaa aagggaattg taaactggtg gctacctgtt
     7081 tgttctatct gttttgaaaa atacataagc aatttctcgc gagttgtttt acgagaaagt
     7141 aattctattt tattgtgaag taaaatgttt ttttcagcta ttaattgtag catattttca
     7201 acaagcctat tatgaaatac acatgcagat gaacaaatac cagttacact tttaaacttt
     7261 ataaataaaa cttcgcaaga agatgttgtg gttactgtaa cattactttt atgtagtcta
     7321 gtacatgcga atacctctcc aaataaatct ccaggcaaga gttcagctac aatattccta
     7381 ttcccttcaa tatcttcctt tattatatga gcacttcctt taagcataat tccaatagaa
     7441 gtaacttcac ttccaacaga gaatattata ctatcttttg ggtaagtctt atctacaaca
     7501 cctaaacatt ccaacatttc ttcaagttca tcacttttaa ttccttcaaa tagaggaatt
     7561 tttttaagta ggtctatatg cttttctaaa aattttttca aataatcact tccttgttgc
     7621 atatgcaacc gataatttgt tttcattgta ctatactaag tttataaaag caaggggata
     7681 taaaaatcct actacaagaa atttgaaata aagaaatata ggtattaaaa ataaatatat
     7741 aagaaatcaa tactttaaat taaaaaaatt ttatgaggtg atataaatga tgagaaaaat
     7801 aattaaaatt gatgaggaaa agtgtaatgg ttgtggacta tgtgttgaag cttgtcatga
     7861 agaagctata ggaatggtaa atggaaaagc aaaactttta agagatgact attgtgatgg
     7921 gctgggagat tgtctgccaa cttgtccaac aaatgcgatt tcatttgagt atagagaagc
     7981 agcagaatat gatgaagctg cagtaaaagc aaatatggaa gcaaagaaag cacaaaagaa
     8041 aactttagca tgtggatgtc caggttctca gtctaagtca ataaatagag aggtttcaaa
     8101 tagtacgtct ataagcaatg atatagtaga gataaaaggt tctcaactta atcaatggcc
     8161 agtacaaata aaattagtac caactaatgc atcatattta aagaatgcaa gtcttttaat
     8221 agcagcagac tgtacagcgt atgcatatgg aaattttcat aataaattta tgaaaaataa
     8281 ggttacatta atcggatgcc ctaaacttga tgaagttgac tatgctgaaa aacttacaga
     8341 gattttaaaa gaaaatgata ttaaaaatat agtagtaaca cgtatggaag ttccttgctg
     8401 tggtggtata gtaaatgctg ttaagacagc tctacaaaat agtggaaaaa tgataccatg
     8461 gcaaatagtt actatatctg tagatggaaa aattgtagat ggagaaattt aattaagtga
     8521 attatagaaa aatataataa attttaataa ataaaaaggg aggaataaaa aatggaaaaa
     8581 atgttttgtt ttcaatgtga acaaacagca ggaggtaaag gttgtactat aaatggtgtc
     8641 tgtggtaaaa aggggggtac agctaactta caagacgaac ttacaggagt gttaattggt
     8701 ctttcaagag caactattgg aaataagaat agaccaacaa gtgaaacaga taaaattatg
     8761 atagaggggc tattcactac tataacgaat gtaagttttg atgatgaagc tataaaaaga
     8821 caaattgaaa aaactgaggt agaaaaaagt aagcttgtac ctagatgttc agattgttca
     8881 acaacttgta atagaaatga taactatgat atgaaggatt tatggaatga taatgaggac
     8941 atacgttcat taaaatcatt aatcttattt ggattaagag gtatggctgc ctatgcatat
     9001 catgcaatgg tacttggtta tacagacaaa gacgtaaatg aatttttcta tgaaggatta
     9061 atagcagttg gaggagactt gtctattgac gaacttttag gtctagttat gaaaactgga
     9121 gaaataaact taaaatgtat ggaattgctt gatagagcaa atactgaaac ttatggaaca
     9181 ccagaaccga ctcaggtatc tatgaaaatc gagaaagggc catttattgt agttacagga
     9241 catgaccttt atgatttaca caaattatta gaacaaacga aagataaggg tataaatata
     9301 tatactcatg gtgagttatt accagcacat gcatatccaa aactaaaaga atataaacat
     9361 ctaaaaggta attttggtac agcatggcaa aaccagcaaa aagaatttga taatattcct
     9421 gctccaatat tatttacaac aaactgttta atgcctgtta aagatagtta tagagataga
     9481 gtatttacta cagaagtagt tgcttatcca ggtatggttc atattgatga agataaagat
     9541 ttttctagtg taatagaaaa agctcttgaa cttggaggat atagagaaga tgttcatatg
     9601 actggtatta atggaggaga tacattaact actggttttg caagaggtac agtttcatct
     9661 gtacaagata aagtagtaga tgctgtaaag agtggagcta taagacactt tttattggtt
     9721 ggaggttgtg atggtgcaaa acctggaaga aattattaca ctgaatttgt aaaacaagct
     9781 cctaaagaca ctgttatatt gacacttgca tgtggaaaat atagatttaa tgatttagac
     9841 cttggggata taggtggctt acctcgttta atggatatgg gacaatgtaa tgatgcttat
     9901 agtgcaatta aagtggcagt ttcattggca aatgcttttg aatgtggagt aaatgatttg
     9961 cctcttagca tggtactgtc atggtatgag cagaaagctg tttgtatact tcttacacta
    10021 cttcatttag gtatcaaaaa tatctatata gggccttctt taccagcatt cttatctaag
    10081 aatgtattag atatattggt tgataaattt gcattgacac ctatatctac tcctgaagaa
    10141 gatatgaaga agattttagg ataaataaat atatctatac ttaaagatat ttatagttaa
    10201 aatattttta agtatgccac aattaagata aggggttaaa cttaaaattt gattaaaaaa
    10261 tcaattttga gatgaccctt tatttatttt ttgtaataat tgaaaaataa acctgctttc
    10321 agtatattct ttttaagaat ctttgtatta aatttataat acttagatat ataatatctt
    10381 atatatttta ttttaagtat taaaggtttg aatgtaactt aaatattaaa agaggaacat
    10441 tgtaatatgt aacttgcata aaatatatat actatagtga aattattttg attaaaattt
    10501 aaaatataag gtgttttatg tgggggtata tgtatatgaa aaattctaat tttaatatat
    10561 tggcatttga tggtggtgga ttaaaagggg cattgagtat atccatactt gagaggatag
    10621 taaaagagta tccaaatcta ttaaacaata taaatatgtt tggaggtaca tctacaggga
    10681 gtctaattgc actagggctt gcgtatggtg taagccctaa agagataaaa gaactttact
    10741 caattgaaaa ttcaaagtat atttttaata aaagttatgc agaaatactc agaccaaagt
    10801 atgaaaacaa aaacttaaaa gaagttttat taagtatatt tccagaggaa ctagaattaa
    10861 aagatttaaa taaattagta atgataccaa gtttttatat aggaaatgag gaaaatgcat
    10921 ggaaaccagt gttttataac aatatgccaa attcttttac aaagactagc aaagttgtag
    10981 atgtagcaat ggcaagtagt gctgctccgg tattttttcc aacatataat cgtcatgttg
    11041 atggaggaat aatagcaact gacccgagtt tagcgtgtat aattcatgcc attgacagtg
    11101 gatttaaact taagaataca agactttttt caatagggac tggatatgta tataacagta
    11161 taaaggcaga tactactgaa tggggagcta ttgactggat aattaataaa gaacccgatt
    11221 taccaattat atctattacc ttagaaggaa attctcaaat gagtcagtta ttttctcaaa
    11281 aacttcttgg agataattac tatagattaa atcctaagat ggaaaaagat gtagctatgg
    11341 atgattgtga tgcattagaa tatctaatgt ctttaggaga aaattgtgat ataaaagatg
    11401 cttttggatg gataaagaaa aagagctttt atagaaatta gattcaattt gcaattgtat
    11461 ttttacacat gatttcattg agtaggtaaa aataataaaa tgcagattat taagttactt
    11521 cttagggaat aaataaagta taatttattt aaggagtgat gaaaatgata aaacttgcta
    11581 cttttgcagg tggatgtttt tggtgtatgg taaaaccttt tgataagtat gatggagtta
    11641 ttaaagtagt ttctggatat acaggtggtc atgttgaaaa ccctacatat caagaggtat
    11701 gttcaggaac tactggtcat ttagaagcaa ttcaaataac atatgatgat gatataataa
    11761 gttatgatga acttctaaat atcttctgga aacagattaa tcctacagat gatggtggac
    11821 aatttggtga taggggaact caatataaga ctgctatatt ttatcatgat gaagaacaaa
    11881 aaaatatagc tttagagtca agagaacatc tggaaaatag taaaatattt gatagtaaaa
    11941 tagtgactga tataattcca gcaactaaat tctacgaagc agaagaaaat catcaggatt
    12001 attataaaaa aagaccatta aactacaata tgtattataa agggtctgga agatataatt
    12061 ttgtaaaaag tcattgggat ggaaacaata taaataggga agagttaaaa aagactttaa
    12121 ctcctataca atttgaagtc actcaaaatg atatgacaga agtacctttt gaaaatgaat
    12181 attatgataa taacaaagag ggaatttatg ttgatattgt aagtggagaa gtgttatttt
    12241 cttcaaaaga taaatttgat tctggatgtg ggtggccaag tttttcaaag ccagttatag
    12301 ataaatctat cattgagaga agtgatttct cacaaggtat gtacagagtt gaagtaagaa
    12361 gtgctaaagg aaattctcat ttaggccatg tatttgatga tggtccaaaa gaacttggag
    12421 gtttaagata ttgtataaat tctgcttctc ttaaatttat accaagagaa aatatggaag
    12481 ctttaggata tggagaatat ttaaaacttt ttgagtaatt aggaatattt attataaact
    12541 tatctaaaag gtgaaaatat aaaaaaatat tttcaccttt tgttttttgt caatagtatg
    12601 ataaattatt aattgtaatc ttatttatac caatagatta agaatttaat tttgaaagag
    12661 agaaaacaaa tgaatttaaa agactttaat ggaaagagat taaaatcagc tagaattttt
    12721 agagcaaaga cgatagaaca gttgtctaaa gaaactaaaa taaataaaaa agatttaaaa
    12781 gcttttgagg aaaataagta tgtaccaaat atagaaaata cattaaaatt atctaatata
    12841 ttaaattttc caaaagagta tttttataaa aatgaaaata taaatatcgt tgtagaggat
    12901 agccacttta accctcaatc taaactacct agagtagaag agatttctta tagagaaaaa
    12961 ataattataa tacataaaat atattcattt atggagaatt atattaagtt tccaaaagaa
    13021 aacttgccaa atagaaaagt aatgtcagat attaatataa atgatataga atctcttgca
    13081 tataagacta gagagttttg ggatttaaat agtactccaa tagtaaatat ggtaagtttg
    13141 ttagagtcta aagggattat aatatcaggt atgaatgtag atagaaaagg ggcaactatc
    13201 tttactcaaa aacagagaat tagtaaagaa tctaaatatc taatatctct gggtaatgac
    13261 aaaaagtcag catcaattag aaattatact ttggcatgtg agcttggata tataatatca
    13321 agtgaattaa gaataccaag taagcaattt tcagaagatg agtatgcctg tgcattttta
    13381 ttgccaaaag agagtttttt aaaagatttg actcatccag aagatttaga ttattatgta
    13441 gaactaaaga aaaaatggat tgtacctata tctgctatga tttttagagc atataatcta
    13501 gaaaagataa attacaaaaa atacaattat cttatgaatg aaatggataa aaaaggctgg
    13561 ttgatagaag aaccactaga caaaatgaaa ggttcaagcc caacgtattt aaaaagagca
    13621 gttgaattat tgatagaaaa taaaataatg agtgtaaata gtatagtatc aagtttagag
    13681 gaatttggaa taaatcttta tccagaagac ttagaattat tgatgggatt gaaaaaagga
    13741 ttgttatctc aagaggtaaa taaaaaaagt aaagttataa aatttgatgg taaaaaatag
    13801 gtttatattt tagtattaaa attaaaagca ggtacaaatg atttaaacat taacttagtt
    13861 aaaagtcttt tcaataagtc atttacctgc ttttagtgta agaaagaata ttgaatctaa
    13921 agtgtttttg aaagtgcaat atattccctt aaaacagaac aagatttatt aaattcgttt
    13981 tgctcatatt cagtcatatt gatttcaata atttcttcaa taccatcttt tcctataaca
    14041 catggaacac ctgcaaatac atttctttcg ttatattgac cttctagtaa tgtagaaact
    14101 ggaagaactt ttttctcatt gtgtagtata gcttttacaa tttctactaa agcagtacct
    14161 ataccaaatt ctgtacaatc tttaccattt attatactta ttcctgtttt tttatttttt
    14221 tcaactattg atggtaagtc taaattgcta taagtacttg ggttgtcttt tatcatgtct
    14281 aagattggtt taccaccaat tgaaacatga gaccaaggaa ccatttgaga atctccatgt
    14341 tcacccatag aataagcttg aattgattgt tgagcgattc cagtttcttc agataaaatc
    14401 cttctaagtc ttgttgaatc taaagctgtt ccagttccaa tgactttgtt tttaggaaag
    14461 ccagattttt cccaaatata atttgtaact acatcaacag gattagatat gactacaaat
    14521 ataccatcaa agccagaatc aactatgggt tttactatag attttatcat tccaatagtt
    14581 ggtcttagta aatctaatct agttgtgttt tgtctacgta aaggcccttc tgaagagtct
    14641 gcaacactta tcactactat gtcagaatct ttacagtcag aaaaagttcc tttttctata
    14701 tgtactttat gtggcagata tgaaactgca tcagctaaat ctaaagcttg agcttttgat
    14761 ttagattcat ctatatcaat cataaagagt tcatcacaaa ctccttgagt tattaaacta
    14821 aatccacaat gagaaccaac atgccctgaa cctacaatag atatttttct tggctttatt
    14881 gccattttaa atcactcccc ctaaaataac gatacattga aaattataga tgttattaaa
    14941 agctgttttt ggatatttta atattctatt atttcagaaa atttatttaa tatgatttta
    15001 tactaaaatt tttaaaaaag caatgttaat atactctcta ttagtgaata ttaaagtatt
    15061 atatgtatat taataattat taaaatattt aatgtcactg atagttagaa tatttaagta
    15121 ttaaaagatg tgtataaata ataatgatgt cagtttataa tgatattaaa ttgaggtata
    15181 ctgtttctct aaattaaaaa ataaaggagt aaatgtgtta tgaattatca tacaaaagaa
    15241 gaattggtgg aagcactgag agtagtatct tcaagtatta ttaattgtgg aaaaggacag
    15301 aagaaatttt ctgaggagac atctcatcat acacgtttta agaatattat agaagtaatg
    15361 tatatttcaa aatcattaat aatggatgaa attagtaaaa gagattgaag cttattttgc
    15421 aatttaacat gttttaatat acattttatt atataagaaa ataagtaaac atacatgtta
    15481 taaaatattg aaataatata caattataaa tttgttaaca ttaatatcta tatgttaaaa
    15541 tatagtagaa gatgttagta aataatagga tgagttctat ttta
//
LOCUS       DAEMHW010000083        15220 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEMHW010000083 DAEMHW010000000
VERSION     DAEMHW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15220
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.083"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            342..1535
                     /gene="nifV"
                     /locus_tag="KPK67_002156"
     CDS             342..1535
                     /gene="nifV"
                     /locus_tag="KPK67_002156"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBG2459122.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1560..3485
                     /locus_tag="KPK67_002157"
     CDS             1560..3485
                     /locus_tag="KPK67_002157"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBG2459123.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKVNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3507..4502
                     /locus_tag="KPK67_002158"
     CDS             3507..4502
                     /locus_tag="KPK67_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBG2459124.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4828..5301
                     /locus_tag="KPK67_002159"
     CDS             4828..5301
                     /locus_tag="KPK67_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2459125.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLIRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5335..5829
                     /locus_tag="KPK67_002160"
     CDS             5335..5829
                     /locus_tag="KPK67_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2459126.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLSLNWSSNKKAMVIHRMAVNPNFRKKGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5908..6447
                     /locus_tag="KPK67_002161"
     CDS             5908..6447
                     /locus_tag="KPK67_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2459127.1"
                     /translation="MKGMIYMIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSK
                     GLRTWEFIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAA
                     SYIQLQAHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEE
                     KKAYSDSDLDYKKVHFNHF"
     gene            6636..6947
                     /locus_tag="KPK67_002162"
     CDS             6636..6947
                     /locus_tag="KPK67_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2459128.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6975..7661
                     /locus_tag="KPK67_002163"
     CDS             6975..7661
                     /locus_tag="KPK67_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2459129.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            7893..8777
                     /locus_tag="KPK67_002164"
     CDS             7893..8777
                     /locus_tag="KPK67_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG2459130.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            8876..10051
                     /locus_tag="KPK67_002165"
     CDS             8876..10051
                     /locus_tag="KPK67_002165"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBG2459131.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            10179..11033
                     /locus_tag="KPK67_002166"
     CDS             10179..11033
                     /locus_tag="KPK67_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459132.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            11077..12312
                     /locus_tag="KPK67_002167"
     CDS             11077..12312
                     /locus_tag="KPK67_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBG2459133.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAMSKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSQFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     YGLAYRINEVILMDELCLV"
     gene            12413..13354
                     /locus_tag="KPK67_002168"
     CDS             12413..13354
                     /locus_tag="KPK67_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBG2459134.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(13405..13764)
                     /locus_tag="KPK67_002169"
     CDS             complement(13405..13764)
                     /locus_tag="KPK67_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459135.1"
                     /translation="MDLFEVNIVLNSGTAGGMNPKLKIFNMVISTEVCHHDVTQDTLT
                     EFHSWLKTVFFEANCELIKISEMAVKETNQEETVFWGRILAGEAFITDKGRKKINEKE
                     AYLHLIASSHIFYTIIK"
     gene            complement(13790..14254)
                     /locus_tag="KPK67_002170"
     CDS             complement(13790..14254)
                     /locus_tag="KPK67_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2459136.1"
                     /translation="MNLGNSLFQARKKHGLSQEDVAEKLGVSRQTVSKWETDETVPDI
                     RQSKKLAILYNVSLDELIDFDIDMEEIQEAINKTSEETEEKINWTNAWGKKYPILLKY
                     QTDVNISNYAYKVNMMLDELKQEYQYNELEAMLILKDILYNVWKNRKSKRKQ"
     gene            complement(14422..14594)
                     /locus_tag="KPK67_002171"
                     /pseudo
     CDS             complement(14422..14594)
                     /locus_tag="KPK67_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
     gene            complement(14674..15027)
                     /locus_tag="KPK67_002172"
     CDS             complement(14674..15027)
                     /locus_tag="KPK67_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG2459137.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
BASE COUNT         5889 a         1613 c         2774 g         4944 t
ORIGIN      
        1 actttaaaat ggaatgtaac ttaaattaaa ttttagtagg aattttatag gtttattttt
       61 aaaggttttt tgaaaataaa tctatttaaa aattaaatta aaatgcaaga aaaacataat
      121 aactttcatt tttaagaagt tttcacaaaa aagaaaaaat atagtgcaat aaatgataca
      181 aagtaatata atagattaca agatatgaaa agttaaaaaa ataaattgca aatgaaatta
      241 aaatgaataa aaaataaaaa aataatcata agttagaggt atcaatgaaa aataagactt
      301 aatgttttta aaattaaaag tatactaagg ggggcattaa tatgtgcgtt atatcaaaag
      361 atagagcaaa ggaaataaaa atagtagata ctactttaag agatggagag caaacagctg
      421 gggttgtatt tgcaaataga gaaaaaataa tgattgctga aatgttaagt gacctaggtg
      481 ttgaccaaat tgaagttggt atacctacta tgggtggaga tgaaaaaaat gtaataaagc
      541 acatatgtag tagaaattta aaatcagata taatggcttg gaatagagca gtaataaaag
      601 atgttgaaga atctatttct tgtggtgttg atgcagtagc tatatcaata tcagtatctg
      661 acattcatat agaaaataaa ttgagaactt ctagaggctg ggttttagaa aatatggcta
      721 aaacagttga atttgcaaag aaaaatggat tatatgtttc agtaaatgga gaagatgctt
      781 caagagcaga tatagacttc ttaactgaat ttataaatgt tggaaaacaa gcaggggcag
      841 ataggtttag atactgtgat acagtaggag ttatgaatcc tttttctata aagaatgcaa
      901 tagaaactct ttatgaaaga actaattttg atatagaaat gcatacacat aatgactttg
      961 ggatggcaac tgcaaatgca cttgcaggaa tagcagcagg agcaaattat gttggtgtta
     1021 cagtaaatgg attaggtgaa agagcaggaa atgcagcatt agaagaagtt ttaatggcac
     1081 ttaagtgtgt ttataaatgc gatttaaata atatagatac tagaaaattt agaggaatat
     1141 gtgagtatgt agcacaggca tcaggaagaa tacttcctac atggaaacca gtagttgggg
     1201 ataatatgtt tatacatgaa tctggtatac atgcagatgg agctttaaaa gacccacata
     1261 actatgaacc atttgaccca agtgaagtaa atcttgaaag aaaaatagta ataggtaaac
     1321 attcaggaag agctgcagtt gtaaataagt tgagtgaata tgaaatgtat atttctccag
     1381 aaaacgctac caagctatta aatgctataa gagctacatc tataagatta aaaagaagtt
     1441 taatggataa agaaattctt caattatatt gtgatatact agcacatgag aaaggcacaa
     1501 cagaagaaga agcagttaga ggttcatata tttaagatat aattgtttag gaggaaaata
     1561 tgggagataa tatagtttat aaaataatta aaaaacatat agtagatgga gaagctgttg
     1621 ctggaagttc aattggtata aaaatagacc aaactttaac acaagattct actggaacaa
     1681 tgacatatct tcaactagaa gctatgggaa tagataaagt aaagactaaa agaagtgtgg
     1741 catttgtaga ccataatatg ttacaacaag gatttgaaaa tgcagacgac cataagtata
     1801 tacaaacagt ggcagataaa tatggagttt atttctctaa gccaggaaat ggtatttgtc
     1861 atcaagtatt tttagaaaga ttttcaacac caggagatac acttttaggt tctgatagcc
     1921 acacacctac tgctggaggt gtgggcatga tggctattgg agctggtgga ttagatgttg
     1981 ctttagccat ggcaggagga gcatactata taaaagcacc aaaagtctgt aaggtgaatc
     2041 tagttggaaa attaaataat atggtatcat caaaagatat aatattagaa gtgttaagaa
     2101 aacaaactgt aaaaggtgga gtaggtaaag tttatgaata tggtggagaa ggtgtcaaaa
     2161 gcctatctgt tccacaaaga gctacaataa caaatatggg ggctgaactt ggagcaacta
     2221 cttcaatctt cccaagtgat gaaaaaacac tagaattttt taagagtcaa ggaagagaag
     2281 acgcatggat agagctaaaa ccagatgcag atgcagttta tgatgaagag attactataa
     2341 acttggatga attaaaacca ttagctgcaa aacctcatag cccagacaat gtagatgaag
     2401 ttgaaaatat aggcaagatt aaaatagacc aagtggcaat aggaagttgt acaaattctt
     2461 cttatgaaga cttaatgaaa gtagcacaaa tcctgaaagt aaataaagta cataaggatg
     2521 tgagtttggt aatagcacca ggttcaagac aagttatgga gatgattgct agaaatgggg
     2581 ctttggcaga tataataagt gctggagcta gaatactaga aaattcttgt ggtccatgta
     2641 ttggtatggg tcaatctcca ggtactgact ctgtgtcttt aagaactttc aatagaaatt
     2701 tctatggtag aagtgggact ttgtcagcac aagtatattt agtaagtcca gaagtggcag
     2761 cagtaagtgc aatcaaggga gtgcttacag acccaagaga atttgatatt aaatttacaa
     2821 atttagatgt aaatgaattt ttgatagatg atagcatgat aattaaacct gctgatgtag
     2881 gaagtgatgt agaagtagtt agaggaccta atataaaacc tttccctctt aatacagagc
     2941 taagtcagtc tataggtgga aaagttatat taaagacaga agataatata actactgacc
     3001 atataatgcc atcaaatgct aagttacttc cttttagaag taatataccg tatcttgcaa
     3061 actattgttt caatactgta gatacagaat ttcctcagag agcaaaagac aataatggtg
     3121 gatttatagt tggtggagat aattatggtc aaggttcaag tagagaacat gcagctttag
     3181 caccattata tctaggtgtt aaaggggtaa tagttaagag ctttgcaaga atccacaagg
     3241 ctaacttgat aaatagtggt ataattccaa tggagttttg tgatgagaaa gattatgaaa
     3301 atatatctct tttagataat ctggagatac caaatatttt agacaatcta ggtagtggaa
     3361 ttttagaagt taagaataca actaaaggaa cttcttttaa ggtcaaagta gaattgtcag
     3421 caaaagaagt tgatgtatta aaagctggtg gaaaattaaa ctacactaaa aatcaagcta
     3481 attaatatga aaagagggaa tattgaatgt ataaagtgac acttatacca ggagatggaa
     3541 taggaccaga agttgcgaaa gctatgaaaa aggtagtaga agctactgga gtagaaatag
     3601 aatgggaaga agttaatgca ggagaggcag ttattgaaga gtatggtact ccacttcctg
     3661 aatatattat agatagtata aaaaagaaca aaatagctat aaaaggccca attactactc
     3721 ctgtaggtaa aggatttagg agtgtaaatg taactttaag acaagcatta gatttgtatg
     3781 tcaatttaag acctattaaa agttttaagg ggataaaatc aagatatgaa gatgttgatt
     3841 tagtggttgt aagagaaaat acagaagatt tatatgctgg aatagaacat aaaataggtg
     3901 attatgcagc tgaaagtata aagataataa caagaagtgc aagtgaaaga atcgttgatt
     3961 ttgcatgtaa ctatgttaaa gataataaga gaaaaaaagt tacagctata cataaggcaa
     4021 atataatgaa aatgtctgat ggattgtttt tagatgtgtt tagagaagtt gcatcaaaac
     4081 atggtgtaga atatgatgat ttgattgtag atgcagcagc tatgaactta gttttaaatc
     4141 cagaaaatta tgatgtaatg gtaatgccaa atctttatgg agatatacta tcagatttag
     4201 gtgctgggct tgttggaggt ttaggtataa ttccaagtgc aaatattggt aaagattgcg
     4261 cgatatttga agctgttcat ggaagtgctc cacaaatagc aggtcaaaat aaggctaatc
     4321 caacagcact tattcaatca tcagtaatga tgcttagata cttaggtgaa tatgaaaatg
     4381 cacaaaaaat agaaacagct ttagaaaaag tatttcttga aggaagtaaa ttgactgtag
     4441 atttaggtgg ctcagcttct actacagagt ttgcggatga ggtgtgtaag tatattgtat
     4501 agattttatt tagggaaaaa gtaaaatata gattgaagga aagaggcttt ctcaaaatag
     4561 agataagcct tttttattgg aatgataaaa tatatttgat tttatacaaa agagagttcc
     4621 tcataaacga aaaagttaat tttgggatat ccattttaat tttaaaatta attgattaat
     4681 atgcaaagaa agattaaata aatttattat ttttaattga actttcaacc aaaatatggt
     4741 aaaatttaaa aaagttgaac tttcaactaa aaatgaatat attgattatg tgaaattcta
     4801 taaaacagaa actatttgac tttagccatg gtagtttcag tggaggtgtg tttgattaag
     4861 actttagata gtaatatatt aagagaagta ggtactttat ctagagcagt aaattcaata
     4921 aatgatatta agtacaagga attaaagtta caaaaaggac aatttacatt tttgataaga
     4981 atctgtgaaa atcctggtat aaatcttgtg gaattatcaa atatgttaaa ggtagacaag
     5041 gctacaacaa ccaaggctat acaaaaatta ataaaggcag gatatgtaga taaaaagcag
     5101 gataagtttg ataaaagagg atataattta actccaactg ataaatcttt ggaagtttat
     5161 gagttgataa ttgaagaaga gaatagaagt atagagatat gttttgataa ttttacagat
     5221 gaagaaaaac aagtggtaac taagttatta gaaaaaatga gcaaaaacgt agaaaatgaa
     5281 tggtttaaag taaagagata gtaaataaat acattaactt aaggagatat ttttatgatt
     5341 agaaaagcga atatgaatga tttagaaagc attatgaaaa ttataaaatc tacagtggaa
     5401 gaaatgaaga cttataataa tacacaatgg gatgaaaact atccattaga aaaagacttt
     5461 gtttcagata ttaagaaaca agatttatat atttatgaag tggatggaga agtagcaggg
     5521 tttatatgtc ttaattatga agagccagaa gaatatttaa gcttaaattg gtcatctaat
     5581 aaaaaagcaa tggtaataca tagaatggct gtaaatccaa actttagaaa gaaaggaata
     5641 gcatctaagt tagttgattt tgctgaaaaa ttagctgtag aaaacaatgt atcatactta
     5701 aagagcgata catattcaat aaactcaaaa atgaatttat tacttacaaa gtgtggtttt
     5761 ataaaaatcg gagaaatgag ttttttagga aaggagaaat ctttctactg ttatgataaa
     5821 atattataaa gagcatttat aatacaaggg gtaaataata gattttcagc attagtaagg
     5881 gcataatata acctagtata tgctattatg aaagggatga tttatatgat tggatttcta
     5941 aagaaaagaa gaagtattag aaaatacaag gatgttgaag tagaaaaaga aaaattagat
     6001 aaaatattaa aagctgctct tttagctcca tcatcaaagg ggcttagaac atgggaattt
     6061 atagtggtag atgataaaga aaagctaatt aatttgtctc aatgtagaac taaaggtggt
     6121 ggatttttct taaaaaatgc acctttagct attgtaatta ttgcagataa agaaaaaaat
     6181 gatgtttgga tagaggatgc aagtatagct gcttcataca tacaacttca agctcatgaa
     6241 ctaggtttag gttcttgttg gatacaagta agaaatagaa tgcatgatga taatatagaa
     6301 gctgataagt acattagaga agaattaaaa gtacctagca agtacagtgt agaatgtata
     6361 atttctatag gatatcctga tgaagaaaag aaagcataca gtgactctga tttagactat
     6421 aaaaaggttc attttaacca cttttagtat ataatatgtt gtattaagac attatatttt
     6481 tttctattgt taatatatac ttaaaattgt aaaaatatat ctgaatttta ctaacttatc
     6541 taggggttat gtacaaggat tttaaagata aatatagaaa ttaatgttta taatcgatac
     6601 ataacttgag taacaaaatt taaggaggtt aaagtatggg tatatttaaa acaaaaatgg
     6661 atgaggactg gaaagtaaac tacattaaag agtttaatga aatgagagat tcttatgaga
     6721 gtaaacttca aaaaaaacag tttgaggtag atagtttgaa atcagaattg gatagactaa
     6781 gaagctataa aaatagcctt aagccaaaag aaaaacaaat tactgatgat gatataaata
     6841 atataaagag tttaagaaga gatggcttaa gctataaaga aatttcaaat caaacaagtt
     6901 ggagcaaagc aactgtaagt agggtattaa atggacttta cgattagcat atacatgggg
     6961 tgatttaaat tgggatgaag tatagttaca aaaaatgcat aatagattct attttattga
     7021 tgtttatagt tcaaatatta aggatgattt tgaattatgt acttctaagt caatttgaat
     7081 ttactcttga aaattttaat attataaact taatttcttt tacattagtt ggtttatcgt
     7141 taattttatt tttaaaagat aattctttgt ataacaaggt tagaaataga aaaatcacag
     7201 aagcttttga agaaaataaa aacaatatac ttattgaaaa gtgtaagctg atactttttg
     7261 ttgtggtttt atctttagca ataattgtaa cttattgtac taagggatat gtacttttta
     7321 atgtaactat gatgactttg tctgtattga tagttccaat atttgaagag ttatttttta
     7381 gagaatatat atggaactat ctaagtaatt ttataaagtc aaagagtaaa attatctgta
     7441 taacatctat actatctgga atttataata taggatacat agatgttata agaaattatg
     7501 taatattgta taacaattcg agctacacct ttgaagttat tatttcaaaa attatgatag
     7561 gtactgtttt tggtattgta cttggattag ttaagtatag gtttagagat gtagggtttt
     7621 gtatattatt gaggagctta tttgctatat ttattaggta gatagtataa tcggtttata
     7681 aggttgtttt aaatttaatt ttaaatcaac cttattttat ttgtgtttta taaagcactt
     7741 atatattaat aactaataaa tttaataaaa attatttgaa tatttttaaa atatataaat
     7801 atgtattgac atataaatat tatgaattac aatttatata aactaataaa aattaaataa
     7861 tattaatcaa cttattgaat ggaggaataa aaatgaaatt tgtgactttt tgtagtagca
     7921 atgaagaaaa aataggtgta tttaattcag aaacaaatag tatatatgaa attaatagtt
     7981 taggattaag taaattatat actgatatga atgattttat tgaaaacgta tctacaggag
     8041 atttagaaaa aataaaaaac aattcatttg aaaacgcaaa gtgttataag ttagaagaag
     8101 ttaaattatg ttccccaatt gtaagaccta aaaaagatat tatatgttta ggtctaaact
     8161 ataaagacca tgtcaatgaa atcccagatg gagttataaa aaacgtagtt atgccagatt
     8221 atccaattta tttttctaag agagcagaca aaataattgg agtggatgat aagataagct
     8281 tacatggaga tttggtagag aagttggact atgaatcaga acttgcagta atcataggta
     8341 aagaaggtat aaatatatca aaagaagatg cttatgaata tatatttgga tatacaatag
     8401 taaatgatat aagtgaaaga gcacttcaag ataaacatgt tcaatggttt agaggaaaaa
     8461 gcttagacac acatacatct atgggacctt gtatagtaca taaagaagaa tttgaacatc
     8521 ctttaaaatt agatataagt agtgtagtaa atggagaagt aagacaagat tctaatacag
     8581 agtactttat atttgatata cctactataa taaatgattt atcaagagga atgactctaa
     8641 agccaggaga tataatttca actggaactc cagcaggggt tgctatgggg atgaatcctc
     8701 aagtatattt aaagcatggc gatatagttg aatgtaaagt cgaaggaata ggagttttaa
     8761 aaaatatagt tgattaatgg ataaatatgc catataattt tattagtacg aataatttaa
     8821 ttaatatagg attaatcaaa cttatgatta atttattata gagagggatg agtatatggc
     8881 aagagaacta gaattaaagt atggctgtaa tccaaatcaa aaaccatcaa agatttatat
     8941 gaagaatgga gaacttccta tagaagtatt aaatggaaaa cctggataca taaatttttt
     9001 agatgcattt aatagttggc aacttgtgaa ggaattaaaa gaagctacag gtcttccagc
     9061 agcaacttca tttaaacatg taagtccagc aggagcagca gtaggggtac ctttatcaga
     9121 tactttaaag caaatatatt ttgttgatga tttagagctt tctccattag cttgtgctta
     9181 tgcaatggca agaggagcag atagaatgtc ttcttatggt gattttatag cactttcaga
     9241 tgtatgtgat aaggagactg caactattat agcaagagag gtttctgatg gtataatagc
     9301 tccaggatat acagatgaag cccttgaaat attaaaagga aagcgtaaag gcaactataa
     9361 tattgtaaaa atagatgaaa attatactcc tgaacctatt gaaacaaagg atgtttatgg
     9421 aattactttt gaacaaggta gaaatgaaat attaataaat gaagacctat taaaagatat
     9481 tccaactgat aataaaattt ttacagatag tgcaaaacgt gatttaatta tagctcttat
     9541 tacattaaaa tatacacaat caaattctgt ttgttatgct aaagatggac aagtaatagg
     9601 ggtaggtgca ggtcaacaat cacgtattca ttgtactaga cttgcaggta acaaagctga
     9661 tacttggtat cttcgtcaac atcctaaagt attaaactta aaatttaaaa aggatatagg
     9721 tcgtccagat agagataaca ctattgatgt atatctttct gatgattata tggatgtgct
     9781 tgctgatggc atatggcaaa acttttttga agaaaaacca gagcctctaa ctggagaagg
     9841 aaaaagagca tggcttaaaa ctttaacagg agtagcactt ggttcagatg catttttccc
     9901 atttggagat aatatagaac gtgcaaaaag aagtggggta agttttatag cacaaccagg
     9961 tggttctatt cgtgatgata atgttatatt aacttgtaat aagtataaca tagtcatggc
    10021 atttactaaa aatagattat tccatcatta aaatactgaa agtataaaga taatatagat
    10081 taagtaaaaa tctacaacaa gttttgttgt agattttttt atttgctgta taattttaaa
    10141 tagaacttta tttttagata ttcaatttgg aggttgatat ggctttaaat ataagaaact
    10201 atagcctatt atgtaatcaa ggaacagcta aatttaatga agatgtagtc ggtataaatc
    10261 catttggggc atgggtttta gatggtgcta caggacttaa tggaaaaaat ttagtgtcaa
    10321 ctgaaagtga cgcaaggtgg tatgttcagt ggtggaatag gtatttatat aaaaatataa
    10381 ataaagaaga atcattaaag agtattatta atactggaat aatagaagta aaaaaagagt
    10441 atgaactaag attaaatggg tttaaggttg aaaaattaga tttaccatct agttctatag
    10501 ttgttgtaaa atttcatgaa aataaagtag agtatttttt attaggagat tgtgctttgt
    10561 ttattaaaaa tggagattcc aaagttataa aagataggag tatttgtaaa tttgataata
    10621 ctgtatttga tgaaatgagt aaactttcta acttggaaga gatggcattt gatgagatta
    10681 gaactagtgt tatggataca atagtaagca atagattgaa gaaaaataca aaagagggct
    10741 attggatatt ggattttgat gaggaagctg tggaaaattc tatatatgga tacattgaaa
    10801 taaaaaaaga ttttcaattg atgctaacga gtgatggttt tacaagtgca tgtgatagat
    10861 ataatctaat aaaagaagaa gaattgatac gtatagcaga aaaatttggt ataggatata
    10921 tacacaataa agtaagggat tttgaagata atgactataa agcggttaaa gtgccaagat
    10981 ttaaagtaaa agatgattca tcttgtttat atttagatat atataatgat tagactgtat
    11041 aaaactattt aattttagaa aggaggcaag tgaaaagtga aaattcttca tataataaca
    11101 caaaaaccaa atagtacagg tagtggtata tatttgtctg gtatgataaa agggttcgaa
    11161 aaaatcggtc ataagcaagc tgtaattgca ggtatagatg taaatgatga tgtaaattgt
    11221 tttccaagtg aagtaagttt ttatccagta aagtataatt gtggggagtt aaattttcct
    11281 gttgtaggta tgagtgattc tatgccatat gaaagtacaa gatataaaga tttaaatata
    11341 gacatgataa atagattgaa atatcaattt aaagtaaata tagataaggc tatgaatgat
    11401 tttaaaccag aacttataat atgtcatcat ttgtaccttt taactgcatt tgttagggaa
    11461 atggtaaaag atataaaggt tatgtctatt tgtcatggaa cttgtttaag acagttaaat
    11521 accattgatt tagagaaaga atacataata gcaaatataa ggaaattaga tttaatattt
    11581 gctcttcatg aaaatcagaa atatgatatt ataaagactt ttggagtaag tgaaagtaaa
    11641 gtagttgtaa ttggtagtgg ttataatgat gatatatttt ataataaaaa ttataaaata
    11701 aaggatgata aaataaaaat agtatttgca ggtaagatat gtaaatcaaa gggattgatt
    11761 ccatttataa aagctatgtc taaattaaaa tatagtaaag atttaataga agtcaatttt
    11821 gcaggtactg gaagcgatat cgaatcatat aatgagatag ttaaactggc aagtaagtct
    11881 caatttaaaa tgaatttcct aggaaagtta gaacagagag atttagctga gctattcaac
    11941 aggtcacaaa tatttgtgtt accatcattt tatgagggat taccagttgt agttttagaa
    12001 gctttatctt gtggcacaga tgtaatcact actgatattc ttggtgttaa agagtggatt
    12061 ggtagtgaga ttaataattc tggaaagata gaatatgttt ctctaccttt tatggagaaa
    12121 gaaggtattc caaaagatga agagttatat gattttgaaa ataatttgta taatgcaata
    12181 gattctaaga tacaatcttt attgaataac tcaaataaga aaacatctgt agatatgtca
    12241 aaaaaaacgt ggtatggctt agcatataga ataaatgaag ttattttaat ggatgaattg
    12301 tgtttagtct aaaattattt aaattataaa aaagtgtaac tatttttaca ctatttttaa
    12361 atgattttat attatactaa tactaaatat atttgtttta gggaggtaat ttatgaggat
    12421 tagtaaaaag gtattggcgt taggtatttc agcattactg ttaagtgtta gttttccaac
    12481 atcaataaac gcactagata agattgaaaa tattcaagga gtagataaat atgaaacagc
    12541 tgggcttatt tcagataagc aagattttac aacagcagtt cttatcaatg cagactctac
    12601 aatggtagat gggattgctg caagtggtct tgcaggagta aataatgcag ctattttatt
    12661 aactaataag gatgatatac cagaagcaac acttcaaaga ctgaatagag ttactaagat
    12721 atatgtaata ggtggagaaa attctataag taaagatgta gaaaaaatgt tacttatgag
    12781 aagaatgcaa gttataagaa ttgatggtgt agatagagta gatacaagtt ataaaattgc
    12841 aggcgaaata gaaaaaatta aaaacagtga taagatgttt ttagtaaatg gatttaagga
    12901 tgaagctgat gcagtaagtg ttgcatctgt tgcttataga gatggagctc ctataatatt
    12961 gacaaaagat ataccttcag cagaagaaga cacagactta gacccatggt ttggagtttc
    13021 tcctgttcca gtatatgcta ttggaggaga gtctacatta agtgattaca tagtgagcag
    13081 atatagagca actagaattg gcggtgttga tagatatcaa actaataaaa atgttattga
    13141 aaagttctat aatggagcaa aagagttcta tataacaagt ggagacgatt tagtttatgc
    13201 tttagttgca tcaccacttg caaaaaatgc tcctgttgtt ttagtttcaa ataaaagtga
    13261 taagagtata ttaagtggag ctagtaaagt aacagcaata ggtataagtg ataagagtat
    13321 tatagagcaa tgtttggatg ctgtaaagaa gtaatggagt gttattagta aaatgaatat
    13381 acacattgaa aattagtaga ttgttcattt tataatggta taaaatatat gggaagaggc
    13441 tatcaaatgg agataagcct ctttttcatt tatttttttg cgtcccttat cggtaataaa
    13501 agcttcccct gctaaaattc ttccccaaaa tacggtttct tcttggttgg tttcttttac
    13561 agccatttca gatattttta tcaattcaca gtttgcttca aaaaatacgg ttttcaacca
    13621 tgagtgaaat tctgtcagag tatcttgtgt aacatcatga tgacaaactt ccgtagatat
    13681 taccatatta aatattttca attttgggtt cattccacct gctgtccctg aattgagaac
    13741 gatatttact tcaaaaaggt caatacttta gtttccaaaa aacggggatt tattgctttc
    13801 gtttgctttt tcgatttttc cagacattgt ataaaatatc tttaagaatt aacattgctt
    13861 ccagttcatt atattggtat tcctgcttta attcgtccaa catcatgtta accttataag
    13921 cataattaga aatattaaca tctgtctgat atttaagaag aatagggtat ttttttcccc
    13981 aggcatttgt ccaattgatt ttttcttcgg tttcctcact tgttttatta atcgcttcct
    14041 gaatttcttc catgtcaata tcaaaatcaa tcaattcatc taatgacaca ttgtaaagta
    14101 ttgctaattt tttcgactga cgaatgtctg ggactgtttc atccgtttcc cattttgaaa
    14161 cagtctgcct gcttaccccc agtttttcag ctacatcttc ctgtgaaagc ccatgtttct
    14221 tccttgcctg aaataaacta tttcctaaat tcattgaaat tcctccttat ctgtttatgg
    14281 gattattata ttatttgata agaacaaact ctaccaacta aaattatcat atttgctatt
    14341 tttattaaca taattcaaag acgtatttat tctgtaaatt aaaatctgct aacctctgcg
    14401 aatatcaatc attaaccact tttatccatt ccctgttttt gtttgtacgt aaatatctct
    14461 taattctggt gtaaactcct gtaaaatcgc ttcgacaacc tctttctctt tctttcaaac
    14521 aacaactaaa acgtaaaata attttctctc ggatatactg tgcataaatt ttttctaggt
    14581 tttttaaatt tgatattcca gccaaattcc caactgcaag aactaaatgt ctattttgag
    14641 acactatctc cttttcttat attatattat tttttatatg gaataaattt cataagtatt
    14701 atcttccttt aatttttcta accaaaccat ctctaaaata tctagttctt ctaaatagtc
    14761 tttctctgtt ttgttatcat catgttttat aagttctaaa acttcaaatg taaatgcttt
    14821 ttcaccatat tttttccatt cattatttaa ttcttttatc atacatgagc cagcaccaag
    14881 ctgaaatttg agagagttaa atttgctttt tatgtcatta ctttttccaa ggtaaatctt
    14941 tttagtaata tcatttttta ttatgaatac acctgtgtca aatttcattt ctttgtataa
    15001 ttgttttaat tcacgttttc tatccatgat tttagatacc tctctttata attgatatgt
    15061 aatatgtaag tgtaaaaata tattacatat caattataaa gagattaatt ttatatgtaa
    15121 atagttttta taaatttaat tatgaattag tcgtcaataa acaagaaatt tagctgtaga
    15181 aatccattat aaaggctatt ataaatttta atatgctaaa
//
LOCUS       DAEMHW010000084        15064 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEMHW010000084 DAEMHW010000000
VERSION     DAEMHW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.084"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            132..809
                     /locus_tag="KPK67_002173"
     CDS             132..809
                     /locus_tag="KPK67_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2459138.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            881..2896
                     /locus_tag="KPK67_002174"
     CDS             881..2896
                     /locus_tag="KPK67_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG2459139.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFEAYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            2946..3626
                     /locus_tag="KPK67_002175"
     CDS             2946..3626
                     /locus_tag="KPK67_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2459140.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            4142..4588
                     /locus_tag="KPK67_002176"
     CDS             4142..4588
                     /locus_tag="KPK67_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459141.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQTQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVS"
     gene            4969..5886
                     /gene="pstC"
                     /locus_tag="KPK67_002177"
     CDS             4969..5886
                     /gene="pstC"
                     /locus_tag="KPK67_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBG2459142.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            5888..6712
                     /gene="pstA"
                     /locus_tag="KPK67_002178"
     CDS             5888..6712
                     /gene="pstA"
                     /locus_tag="KPK67_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBG2459143.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            6731..7495
                     /gene="pstB"
                     /locus_tag="KPK67_002179"
     CDS             6731..7495
                     /gene="pstB"
                     /locus_tag="KPK67_002179"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBG2459144.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            7523..8176
                     /gene="phoU"
                     /locus_tag="KPK67_002180"
     CDS             7523..8176
                     /gene="phoU"
                     /locus_tag="KPK67_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBG2459145.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            8334..9203
                     /locus_tag="KPK67_002181"
     CDS             8334..9203
                     /locus_tag="KPK67_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG2459146.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            9290..10726
                     /locus_tag="KPK67_002182"
     CDS             9290..10726
                     /locus_tag="KPK67_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBG2459147.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKEALHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYAITGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            10774..11601
                     /locus_tag="KPK67_002183"
     CDS             10774..11601
                     /locus_tag="KPK67_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2459148.1"
                     /translation="MKIKDNKAVFHVFIRIFIIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    11897..12159
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12235..14901
                     /locus_tag="KPK67_002184"
     CDS             12235..14901
                     /locus_tag="KPK67_002184"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBG2459149.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLVNQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         5952 a         1567 c         2753 g         4792 t
ORIGIN      
        1 gtattatagt ctagtttgta aagatattat aaaataataa taccagttat atatgaaaat
       61 aaaacattat aatttgtaat aactataaaa cttggaatta taattaaaat acctttgaat
      121 ggagtggtgt aatgtataat ttactggttg tagatgatga tgttgatatt ttaaacataa
      181 atcaaattta tttttctaat ataggtttta atgtatatac atcagagact gctaaagatg
      241 ctatggatat agtagagagt atagttttag actgtataat tttggatatt tcattgccag
      301 atatagatgg atataaaatg tgcaatgata taaaagaaaa ggttaacata cctataatat
      361 ttttatctaa ttatgagcat gaagaagaaa aagttcgggg atttttagct gggggggatg
      421 attatataac aaaaccttat agtttaaaag aacttgaact tagaatatat gctcgtatga
      481 ggcagtacaa aaatattaca tcagttccta atgtgttaaa gttttcagcg ttagtaataa
      541 atgtaaattc aagaaaagta acttttgatg gagaaagtat agatttaact actatggaat
      601 ttgaaatatt attgtttcta gctgagaaca aagaacaagt attttcaaag atagagatat
      661 ataaccaagt ttggaaaatg cctgatattg gagatcaaca tacagtgcag gttcatattg
      721 cacagatgag aaaaaaaatt aattctttaa gcagggagca tcaatacatt caaaccgtat
      781 ggggaaaagg atataagttt gtaccataag tgagatactg taattaataa atagttcttg
      841 ttttatttaa tacgactatg aaagagatgt ggttgatgca atgaaaaagt ataatatact
      901 aaaaggaact ctcattgtat tgatagtctc tctgttggca ataacaggat taaatcttct
      961 gtataaatat gataataaat atacatataa gtctattaga gcaagtaatg gaattttaac
     1021 tatagataat gatactttca ctaaagggaa acttgtcttt ttgatagatg actgggagtt
     1081 ttataatcat aaaatattta aaccgaatga ttttacagag caagatataa aaccagaata
     1141 tgtttttata ggtcaatatc cagatttttc tatgagaaag gggaatcagt caccatatgg
     1201 gaatgctact tatcgtatga gaataaaaaa tgaaggcaag gataagctac taagtttaga
     1261 gcttcctgag attttttccg caagtgaagt atggataaat ggagaaatgg tttctaaatt
     1321 aggagatgta ggaacagaaa aatatagacc caaaattaaa aattcagtag taagttttat
     1381 tgcaaagaaa aatacagaaa ttataatcaa tgtatcgaat ttttcacatt attatagtgg
     1441 tttgtattat ccgcctgcat taggagaaac aaaggacata agtaatatga tgttttatcg
     1501 attacttttt tatagtataa tttgcttcac aacttttgca atagctatat tttcattgtc
     1561 cgtatggttt ctttcaaaca aaagtaaaat ttacttatat tttggatgta tgtgtgtttt
     1621 ctttgccatc catgtatcct atccctttgt acatttgtta ggactgcctt tggtaagctt
     1681 tacatatgcg gttgaagatt tgtcatattt tatggtagtt ttatgtatta tttgtataaa
     1741 tggaagtata tccaaaatag aagaaaataa agtgtataga tttttaattt taccattagg
     1801 aattacaatg agtatcatat ctatagtgat accaattatt cttcttccat atgatatgta
     1861 ctttgtcaac atatatggga agttaataga catatataaa tatattgtat ttatatatat
     1921 attaatgtct tcgctggttt caatatataa aaagacatgc tctgagcata taagttctta
     1981 tattttagtt tcagtaaaca ctgtttttgg aattagcata ttatttgatg cagttacgag
     2041 taatagattt gagcctatat ttacagggtg gcaaacagaa tattgtgggt ttgtaattgt
     2101 tattttattt tctgttatga tgataaacag gaatagaata ataataaaag agaatgaaaa
     2161 acttacaagg aatcttgaac atgaagttga agtgagaact aatgaactta caacattgtt
     2221 aaatgaacgt aaaagattct tggcagatgt agcacatgat ttaaaggcac ctgtttcggc
     2281 tatacaagca tttatagatt tgataaaggt tggtaatatt catgtagatg aagagacaag
     2341 aacctatctt tatgctataa atcaaaagtc aaatgaagta caaaatagag tacgtagttt
     2401 gcaagaattt acatcacaag ataaaagcat tggtattagt gaaaaaatat gtctgaataa
     2461 ttttattaaa gagatatatg aaaataattt gccagataca caagcatgtg gtgtaaactt
     2521 tgaagcttac gtattggaga aaaaaatata tgcaaagata aataaagaga tgcttacaag
     2581 agcatttgag aatttaattt ataatgcttt gacctttact ccatttgatg gaaagataat
     2641 tttatcaatg gataaagaag atggatttgc aattattaaa atttctgata atggaaaagg
     2701 tatacctcca gaaaatatac caaaggtatt tgacagattc tttacagaaa gagacaataa
     2761 tgagccaaaa gggcaagggt tagggctata tattgtcaaa tccattataa gagagcatgg
     2821 tggagaaatc tttgtagatt cagaactagg aaaaggaact gtttttacaa taaaattaag
     2881 tctatgtttt ttataataat agcttattta atataaaaga ataatgatat ttgggagtga
     2941 ttataatgca aactaagata ttgataatag atggagacaa ggataattgc cagaagctaa
     3001 aaggatttct ggaggaaaaa ggaataagta tagatttagc atataattgt gaagaagcta
     3061 ttggaaaaat atttagcaat aaatatgatt taatattttt agaaataatt ctaagtgatg
     3121 gtgatggatg gactctttgt aaaaaaatta gaaatgtaac tacttgtcca attgtttata
     3181 tgacttatat aaatgaagac caaagcattt taaatgcttt aaattctggt ggagacgatt
     3241 acttaataaa gcctctaaat ttagaaatat tatatgcaaa agtaaaagct attttaagaa
     3301 gaatgaactc atatgtaaat aataatgaaa ataatacaaa attagaggaa tataatagaa
     3361 ctagtggagt aataaagtta gaagataaaa taataaaatt gactccaaca gaaaataagt
     3421 tgttaaactt ttttatagag aatcctgaaa agactttgac tactaaagag atatatgaac
     3481 atgtatggat gaatgaatac ttagaggata actatagtgt ggtagttgca gtgaatggat
     3541 taagaaagaa aatagagaaa gattataaaa atcctcagaa aattataaca ataagaggag
     3601 caggatatta ttttaataaa gaataataaa tatattaata tcattaataa ttgttataaa
     3661 tgtgaaatac taacgatatc taaactaaat tattataaaa tagctgactg taatttaatt
     3721 gataaagcct attcttctag actttgccag ttaaattaca gtcagtttta ttatataaaa
     3781 aaatgcgtaa cggaactatt attttataga ttatatttga aaagataaat aaattgtata
     3841 tctaaatcta tatattattt tattatttaa tatatattaa ataataaaat agttaaataa
     3901 taagattaag aatttcttaa taaatccaga agatgctttc gattatataa tattttatat
     3961 ttttatatta ttaattatat atgttgcaat tgctgcacct actttttctg gtgagtatca
     4021 gacacaatca gatagcatcc ttcgttgtac gaaacatgga catattcgtc tagcgtttac
     4081 taaaatatta gctctatttt ctatatttat aataatgttt gttatatgta tttctatcca
     4141 tttggcaatt tctgatttag cttttggtac tgaatgtttg aaaacctcat ttcaaatgtt
     4201 gttttctgtc attagtctag taaatataaa tttactacaa acacagattc ttatagctat
     4261 tggtggtcta atttcaatac ttgcaatggt tagtttaaca ttatttttat ctgcaaaatg
     4321 taaggattct cttacttcta tgctgattgc tttttctatt tgcttaattc ctatgggaat
     4381 aggtgttagt tggattggat acctattacc accaggggga attggtttta agattagttt
     4441 attatatcaa atgacatcat ttaatttctt acacttagga ggtatgagtt tttggacacc
     4501 acacattata ctgtttttct cggtgataga aatacctatt tttatatttt tggctattcg
     4561 tacctattgc aaacatcaag tatcgtaaca atttgatgga aataatagtg atataaacta
     4621 gaggatacaa taattatata tgtagtataa aagttaaatt tagttaacaa atcttaatag
     4681 cagatatagg taataaatta tacataaact gtattatacc tatatctgct attttaatat
     4741 agagaaacaa cagtatattg taaagtaaat gtcaaatata gttttaaaag taaaattaat
     4801 gttaaaaaag taaatataat tttacacttg cttaacttaa tcctaatata gatttaactt
     4861 aaaaaaagta gaataaaagt ataggaaatt tgatgaagac gaattataaa aaattaaatt
     4921 ttgaatacag aacgaaaatt gataagtata ttttaatggg agggtattat gatagtaaaa
     4981 tctagattag taggtttaga aaaaaatgac aataatggca ataagacaaa atacgtaatt
     5041 gaaaaagtag caaaaaatat ttttctactt agtgctttag tagcagttgc aagtttactt
     5101 ttgataatag gattcgtatt ttataaagga ttaagaccat ttatatttga aggatattca
     5161 tttatagact ttttaactgg tgcagattgg gttccttctg ccaataaatt tggaatatca
     5221 tctatgatag tagcatcaat atttgcaaca cttggagcac ttgtgatagg agtacctgtt
     5281 ggtatattaa cctctgtatt tatagcagaa gtagcaccta aaagattagc aaaaataatg
     5341 tcaccagcag ttgaactttt agcaggtata ccttcagtat tatatggtgt atttggtctt
     5401 gcagtgatag ttccaactat acaaagtata tttaatcttc caaaaggtca aagtttgtta
     5461 gcaatagtaa tagtattagc agtaatgatg ttaccaacaa taatatctgt atcagaaaca
     5521 gcaataagag cagtaccaaa agcatacaaa gaaggttcat tagcacttgg tgcatcaaaa
     5581 atagaaacaa tattcaaagt tgtattacct gctgcaaaat ctggagtatt agccgcagta
     5641 gtgttaggag taggaagagc tttaggtgaa acaatggcag taatacttgt tgcaggcaac
     5701 tcaccagtta tgccaactgc aataacagat agtgtaagac cacttacaac taatatagca
     5761 cttgaaatgg gatacgcatt tggtacacat caagaaatgc tatttgcaac tggagtagta
     5821 ttgtttacat ttatattaat tttaaactta gttttaaata aactttcaaa taaggcggta
     5881 aattaagatg agaaagttca aagagaatct attgaagtca ttagtatatc tatcagcact
     5941 atttacaata gtatcattag tagttatagt gggatttata tttataaagg gaattggcaa
     6001 catgaatttt aatttcttat ttagcaatta ttcagcatca ggtgatggtg gtatacttcc
     6061 aatgataatt acaacattat atacagtagt agtttctata gttgtagcaa ctcctatagg
     6121 aatattggct gctatatatt tacaagaata tgctaaaaaa ggaaaagcag ttacagctat
     6181 aagatttgca actgaaagtt tatcagggat accatctata gtatatggat tatttggagg
     6241 aattttcttt gtagttacat taaaaatggg atattccata gttgcaggtt cacttacagt
     6301 tgcaataata atattaccag taataattcg tacaacagaa gaagctttaa aaacagttcc
     6361 tcaagcttat agagaatcat cattggcatt aggagctaca aaatttcaaa ctttatataa
     6421 ggtagtactt ccaagtgcaa taccaggaat tttatctgga gtaatcttat cagttggtcg
     6481 tataattgga gaatcagctg caatattact tacagcaggt actgtagcca agatgccagg
     6541 tggaatattt gatagtgcaa gaacattaac tgtacactca tacttactga ctaaagagag
     6601 tggagatata gcaacagcag ccagtatagg aattgtttta atagtaattg tgttagcact
     6661 aaacatgcta gcaagatttg ttgctaagaa attaaataaa gctaattatt aaatttaaga
     6721 ggtgtgaata atggaattaa tcgataagat aaaaatgagt gtaaaagatt tggatttatt
     6781 ttatggtgat aagcaagcat taaagaaaat aaatatggat ataaaagaga ataaggttac
     6841 tgctttaata ggtccatctg gatgcggaaa atcaactttt ataagaacgt taaatagaat
     6901 gaatgactta atcgaagatg taacaattaa aggaaacatt tctgttgatg gagaggacat
     6961 atatacctca gatgatgtta taaatcttcg tactaaagta ggaatggtat ttcaaaagcc
     7021 aaatccattt cctatgagta tatatgataa tgtggcatat ggtcctcgta cacatggact
     7081 aagagataag aagcaattag ataaaattgt agaagaaagc ttaaaaggtg cagctatttg
     7141 ggatgaggtt aaagacaggc ttaaatcatc tgcactagga ctttctggtg gtcaacaaca
     7201 gcgtatatgt atagcaagag caattgctat gagaccagaa gttattctta tggatgaacc
     7261 tacatctgct ttagacccaa tttcaacatt aaaagtagag gaacttatag aggatttaaa
     7321 aaaagactac acaatagtca tagttacaca taatatgcaa caagctgctc gtatatcaga
     7381 tgaaacagct tttttcttaa atggagaggt tatagaattt agcgatacaa aaactatgtt
     7441 taccactcct gtagataaaa gaacagaaga ctatataaca ggtagatttg gttaaaatat
     7501 ataactataa aggagttaga caatggtcaa taatagttta gatataagta ttgatacttt
     7561 aaaagaacaa acagtgctta tgatggaaaa atgtgaagaa gcaatagaaa agtctgtgac
     7621 ttgtatgata gagaaggatt tagagggtgc aagaaaagta gtagaagatg atgaagaaat
     7681 aaataaatta agagaagaaa taagagataa gagtatagag ctaatagtat taaagcaacc
     7741 aatggcaagg gacttaagat ttatttattc attaggtaat atatctatag agctggaaag
     7801 aataggagat tatgcagcaa atatagctag agaaatgttg aagataggtg gagaggctta
     7861 tataaaagag ctaatagata ttcctaagat ggcaaaagaa tgtaagtcta tgatgtctca
     7921 gactagagaa gctttaatct tatttgatgc aagtatagct tatgatgtcg gaactaaaga
     7981 tgatattgta gatggattgt atgaacaagt acaaattgat tgtttaaaaa taatgcatga
     8041 aaactcaaat accatagacc agggggtaaa acttctactt ataggaagat accttgaaag
     8101 aattggagac catataacaa atgtatgtga gaagataatt tttgcaattg agggtaaaat
     8161 ggttgagata ggataaaaaa ctgctttaaa gcagtttttt tattgtctaa attaagaaag
     8221 aattgataaa aaaatatgat taatgggtaa atttaaaaaa ttgtgatata ctaggaacat
     8281 attaaatatg aggatgcgta ttttatataa ggtgcttttt aggaggtgga actttgaaac
     8341 ttcaaaagat gggtatgagg actataaaaa caggaattag tgtcacttta tgtatgattc
     8401 ttggtgacta tttagtagag aatatgtttt attctctggt agcatgtata atttctgttc
     8461 aagatacagt aaaaggttct ttaaagacgg gaatcaatag agtggaaggt actattttag
     8521 gtggtataat aggattcata tttgccatca ttaaagctgg agacccattt ttatgtggtc
     8581 taggagtaat gtgctcaata tatttatgca atctgtttaa aataaagtca gtagtagttg
     8641 cctgtgtaac ctttctggct atacatctag gtgtaggaaa tagtgaccct gtgtattatt
     8701 ctttacatag ggtgttagat acatctgtgg gtgttttgtt tggagtactt gttaattatt
     8761 atgttgctag accaaattat ctaaatagtg ttatagatga gtttaaaaag atagaagaac
     8821 tttcaatgtt gcttattgaa gataaaattc ttagaaaaga aaatttaaat gtagagaaat
     8881 ttgaagaaga agtagaaaaa ttagaagaaa tatattcaaa acttattgat gagttggatt
     8941 atagtttgag tgaaactaat atagaaaaaa ttgagaatgc cttaaggata tgtagagaaa
     9001 tacattttca tatgcagtct atagagttac ttgaaaataa actttatttg aaagagcata
     9061 catataaata ccttaagaaa acatataatg tggataagct agactggaat atagatgaag
     9121 aaaaaagccc tgtattcaac tatcatctaa tgaaaataat gattgaaata gagttgttga
     9181 gaaagctaaa taaatcatat taataatagt agttaaataa taaataaaaa atagtatctt
     9241 tgtaaatatt aaacatatat aagtttaatt tatggaggta ctatttttta tgaatactag
     9301 aaatgacaat tctaaaatac tagaaatata taaaatgaag ctttttggcg aacttgtaaa
     9361 agcagtgtgg aatgatgata tagaggatat tgaaaactta cctaataaaa ttttaaatga
     9421 agatttaaag gataatttaa ataattattt tagtggaaaa tttgataaag atactatggt
     9481 aaatttaatt agggtaacta tgggattgga tgtgacagaa gatacagata aaaatttaaa
     9541 agaggcacta catgaagctg taaatttaga tgaagtcaaa agaccaataa tatctgtaat
     9601 atcagatgct tgcaaatatt gtgatgaaaa taataaggaa gatgagtcat gtaagataag
     9661 aagtaagcat attaattgta atgaggaaga tgtatgtagt gcttgtggtg attgtgtatc
     9721 aagatgtaag ttgggtgcaa tttcagataa aatagaattt ataccaatga taaaattgtt
     9781 aaaagatata aagagccctg tatatgcaat agttgcacca gcttttgtag gacagtttgg
     9841 gaaagatgta agccctggta aattaagaac tgcattaaaa aagataggat ttgaagatat
     9901 gatagaagtt gctttggcag cagatatgct tactgcaaaa gaagcatatg attattatga
     9961 gcatatgaaa aaggacgaag aaggatgctt tataactagt tgttgctgtc ctatttgggt
    10021 tagtttgata caaaatagtt ttcctcagat attggagaat gtatcaccat ccgtttctcc
    10081 tatgattgct tgtgggagag ctgtaaaaat tttaaatcca gatgctaaag ttgtattcat
    10141 aggtccttgt atggcaaaaa agaaagaagc aatgttagaa gatataaaag atgcagttga
    10201 ttttgtgctt acatttaaag agttgcaaga aatatttgat gcattagagt taaatccagc
    10261 aataatggat gagagtaata gagtagaggc gtccctttca ggtagagttt atgctataac
    10321 aggaggtgta agtaaagcag ttaagttatc agttagaaga attgacaaaa atataaaatt
    10381 tgaagaaaag gcatttgaag gaacaaaaga gtgcatggac ggtctaaaga aagttctaaa
    10441 taaagaaata gaagctacat ttatagaagg aatggggtgt gtaggaggct gtgtaggagg
    10501 tccgaagcgt attttaagtg tggaagaagg tactaagaat gttgatgaat atagtgaaag
    10561 tacgaatatg gaaactccat ttgataattt aaatgtatta caatttttaa cttctttggg
    10621 aatcaaaaga gtagaatctt taggagaaaa agaagaagaa aaggttaaaa aaatatttag
    10681 tagagatata aaaagtaata agaaagagaa taaatataaa aaataacttt ttaagtctta
    10741 aacttatgct tttataaaag ggagatatgg gggatgaaaa ttaaagataa taaggctgtt
    10801 tttcatgtat ttataaggat atttattata gctgttttaa ttttaactgg aacattattg
    10861 gaggaaaaaa ttaataatgt tccagttgaa aatataagtt caaaaggtga aaatacactg
    10921 gaactgcttt ctcaaagtaa tactttagga gaaatcatat acaaagcatc tcaagaagaa
    10981 acaaaaggtc caggggctac ttctgaaaaa gtagcataca taacaataga tgatggtcca
    11041 tcaaaatata caaatgcact actggatatt ttaaaagaaa atgatgtcaa agcgacattt
    11101 ttcatgctta atcagaatat gaaaaaccac aaggaagaag taaggagagt cttggaagaa
    11161 gaaaactcaa taggttttca tagtgtaagt catgatatac ataagttata taaaagccca
    11221 gaagtaacag ttggggaatt taatacatgc aataatactc tacaagaaat aactggtcag
    11281 agttcaaaat taataagatt accatacgga agtaaaccat atatgccaga aggttcatat
    11341 aataaactta tggcaaatga ttatttaatt tgggactgga atttggatac acaggattgg
    11401 aagtcttcta caagtcagat agtaagtaat atattatatt atggaagaaa aagagatgaa
    11461 ttagtagtat taattcatga gaaagagcaa agtttaaatg ctttaaataa tattataaga
    11521 atcttaaaag agagaggata tacaatattg ccaattacag aaaatataac acctaaaaat
    11581 ttctggcaag aaaacttata ataaatttaa atatagtaaa agataaagta aattatttat
    11641 aaaataaaac atataatata aaaaatacga ataagaggta tactgtaagg ataatataaa
    11701 attaatgaga aagcattaaa attaaaataa aataatcttg caattgttga cattataaga
    11761 cagaataact gtaaaaacat attttaaaat ataacaacta tataaaagag aatagacata
    11821 attaagaaaa ttcagaaaaa ataaaataaa aataattgac aaagagaata atatttgtat
    11881 ataataatat gtaatattaa agactgagaa gaagaggagt aagtaaatac tactaaaaga
    11941 gaggaaaatt cactggctgt aagattttct aagtataaga tttactgaat gtagctttgg
    12001 agtttctaaa ctgaactata agtaagttta gacggttaga accgttaaat tctttgagag
    12061 tcaaagttga taaaataaca ttaactaata atttaattat atgttaatgt attttaagct
    12121 ttggaattaa ggtggtaacg cgagcttttc gtccttttta aagaggatga agagctcttt
    12181 tttatttcta aagatattta aaaaatttat ttataaaata caggaggcag gacaatggaa
    12241 aatacgaatt tatcaaaaac ttataaccca aaagattttg aggctaggct atataaaaaa
    12301 tggatggatg aagggtattt taaatctaaa ccaaatccag ataaaaaacc atttacaata
    12361 atgatgccac caccaaatat aacagggcaa ttgcatatgg gacacgctct tgaccataca
    12421 cttcaagata tattaatcag atggaaacgt atggatggat atgaagcttt ttggcttcca
    12481 ggaactgacc atgcttctat agcaacagaa gttaaagttg ttgaaagaat aaaaaaacaa
    12541 gaaggcaaga caaagtatga aataggtaga gaagaatttt taaagagagc ctgggaatgg
    12601 aaagatgaat ttggaggaaa aatatctaat cagcttaaac aactaggtga ttcttgtgat
    12661 tgggataaag aaagatttac tatggatgaa gggtgtaatg aagcagttat agaatttttt
    12721 gtaagcctat atgaaaaagg tcacatatac agaggaaata gaataataaa ctggtgccca
    12781 gattgcaaaa ctactttatc agatgcagaa gttgaacatg aagaacacga cggaaacttt
    12841 tatcatataa agtacccact aaaagatagt gaagactttt tagaaatagc tacaactaga
    12901 cctgagacaa tgataggaga tactggtata gcagtaaatc cagaagatga tagatataaa
    12961 catttaatag gaaaaacagc aatacttcca ttagtaggaa gagaacttcc aatagttgca
    13021 gatagttatg ttgatttaga gtttggaact ggtgcagtta agatgactcc agcacatgac
    13081 cctaatgatt ttgaagtagg attaagacat aatcttgagc agttaaatac tatgaatgaa
    13141 gatggaacta tgaatgaagt ttgtggaaaa tatgaaggta tggatagatt tgagtgtaga
    13201 aaagctatcg tagcagactt aaaagaacaa ggttacctaa ttaagataaa agagcataat
    13261 cataatgtag gaacttgtta tagatgccat acagtagttg agcctagatt atcagagcaa
    13321 tggtttgtta agatggaaga acttgcaaaa ccagctatag atattcttaa aaaaggagaa
    13381 ttagaatttg ttccagataa atttgataaa acttatttac aatggctaga aaatataaga
    13441 gattggtgta tatcaagaca attatggtgg ggtcatcaaa taccagctta ttattgtcaa
    13501 gagtgtggag aaatagtagt tgctagaaaa atgccagata agtgtcctaa atgtggaagc
    13561 actcatttta aacaagatga agatgcctta gatacttggt tcagttctgc attatggcct
    13621 ttctctactc taggatggcc aaataagact gaagctttag attattatta tccaacaagt
    13681 gtacttgtta caggatacga tataatattc ttctgggtag ttagaatggc atttgcaggt
    13741 atgttctgta tgaatgaaaa gccatttgac catgttttgg ttcatggatt agtaagagat
    13801 tctcaaggta gaaaaatgag taaatcttta ggaaatggta tcgacccatt ggagataatc
    13861 gagcaatatg gagctgatgc cctaagattt acattaacaa ctggaaattc cccaggaaat
    13921 gatatgagat tctatatgga aagagtagaa tttgctagaa actttgcaaa taagttgtgg
    13981 aatgcatcaa gatttgtatt tatgaatatt gatgaagata taattaaaaa catgactaga
    14041 gaaagtgtta aagaggactt aactctagct gataaatgga taatatcaag agcaaataat
    14101 attgtaaaag aagcaactaa taacatggat aagtttgatt taggaatagc acttcaaaag
    14161 atatacgatt ttacttggtc agaatactgt gactggtata ttgaaatggt taaacctaga
    14221 ttatatgggg aagatgcaaa tgctaaatct gcagcattat atacattaac ttatgttctt
    14281 gaaaagatat taaaattatt acatccatac atgccattta taacagagga aatatatact
    14341 catcttccta ctgttgaagg atgtataata gtatctgaat ggccaaaata taatgaagaa
    14401 gataacatgg ctgaagaaga agatatgatg aatttattga tggaaggtat aagaagtata
    14461 agaaatgtga gagcagaaat gaatgtacca ccttctaaga aagctaaatt gataataatc
    14521 ccaagtgaag aaaaaataga agctattgag cttggtaaag actattttat aactttagca
    14581 tctgcttcaa atgtagagat agcaaaagat aaatctaatg ttccagaaga tgcagttgga
    14641 gtagttattg atggtgttga aatatttata cctcttaatg aattagttga ttttgaaaaa
    14701 gaaattgaga gattgtctaa agaaaagaaa aagttagaag gcgaaataaa gagagttaat
    14761 ggaaagcttg taaatcaagg tttcttagca aaagctcctg aaagtttaat tgaagaagaa
    14821 aaagcaaaaa aagaaaaatt tgaagagatg attaaatctg ttgaggaaag attaacaaat
    14881 ttagaatcta aaataaaata aatataagaa ttagtaaaaa catacaaaaa gtatatattc
    14941 tatacttttt gtatgttttt ctttatatat atacaaatat gatgttataa ttctttacag
    15001 atgtgttata tttttatttt atgttgtgaa tatatgaatt ttatgtctta aaatttataa
    15061 gcaa
//
LOCUS       DAEMHW010000085        14816 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEMHW010000085 DAEMHW010000000
VERSION     DAEMHW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.085"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            319..1479
                     /locus_tag="KPK67_002185"
     CDS             319..1479
                     /locus_tag="KPK67_002185"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBG2459150.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            1481..2611
                     /gene="glgD"
                     /locus_tag="KPK67_002186"
     CDS             1481..2611
                     /gene="glgD"
                     /locus_tag="KPK67_002186"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBG2459151.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            2640..4082
                     /gene="glgA"
                     /locus_tag="KPK67_002187"
     CDS             2640..4082
                     /gene="glgA"
                     /locus_tag="KPK67_002187"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBG2459152.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            4096..6516
                     /locus_tag="KPK67_002188"
     CDS             4096..6516
                     /locus_tag="KPK67_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBG2459153.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMIKELLPRIYMIIEEINRRYVE
                     ELNNKGYEQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            6521..8386
                     /locus_tag="KPK67_002189"
     CDS             6521..8386
                     /locus_tag="KPK67_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBG2459154.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRSYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            8631..9284
                     /locus_tag="KPK67_002190"
     CDS             8631..9284
                     /locus_tag="KPK67_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2459155.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            9380..9979
                     /locus_tag="KPK67_002191"
     CDS             9380..9979
                     /locus_tag="KPK67_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HBG2459156.1"
                     /translation="MYPNKEIAIEELEVAGKMNPGPWIKHSYNVANVAKLIAEQCENL
                     NSEKAFVCGLLHDIGRRTGISSVRHIIDGYDYVISKGWDEVARVCLTHSFPVKNIEAD
                     IGNKDISKEQYDFINHYLNNLEYNDYDKLIILCDALADANGFCILEKRFVDTTRRYGI
                     YPFSVERWNKTYEYKEYFEKIINKSIYTLLPNIEKSIYY"
     gene            10576..12051
                     /locus_tag="KPK67_002192"
     CDS             10576..12051
                     /locus_tag="KPK67_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG2459157.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIKLCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            12138..12551
                     /gene="speD"
                     /locus_tag="KPK67_002193"
     CDS             12138..12551
                     /gene="speD"
                     /locus_tag="KPK67_002193"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBG2459158.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            12581..13432
                     /gene="speE"
                     /locus_tag="KPK67_002194"
     CDS             12581..13432
                     /gene="speE"
                     /locus_tag="KPK67_002194"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBG2459159.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGAVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            13422..14300
                     /gene="speB"
                     /locus_tag="KPK67_002195"
     CDS             13422..14300
                     /gene="speB"
                     /locus_tag="KPK67_002195"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBG2459160.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNILNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(14480..14680)
                     /locus_tag="KPK67_002196"
     CDS             complement(14480..14680)
                     /locus_tag="KPK67_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG2459161.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         5955 a         1506 c         2636 g         4719 t
ORIGIN      
        1 aaattaaaaa ataagcatgt tcatatgaca caagggattt ataaaaaatg tttttatatt
       61 tataaaataa aatttgtatt attttttaac cgataattga atattaataa atactatagt
      121 gcttatggaa aataatgtat acgtcggtaa gtccattatg gggtatacat tatatcaatt
      181 gtatagttga tattatttta tttttattaa ggtattattt aagcaacatt tatacacgac
      241 atttattgac tgataaagtt tataaaagta aaaatgcaat tataaatatt aattactaga
      301 tgaggggagt gtcattaaat gaaaaaagag atgttagcta tgattttggc aggagggcaa
      361 ggttcaagat taggtgtttt tacaaaaaga attgccaaac cagctgtatc atttggaggt
      421 aagtatagaa ttatagattt tgtgttaagc aattgttcaa actctggtat tgatactgta
      481 ggtgttttaa ctcaatatag accgttgata ttaaattcac atataggcat gggaagtcat
      541 tgggatttgg acagaataaa tggtggtgtc tatgttttac aaccatttat gaatgaaaaa
      601 gaaggaaatt ggtataatgg tacagcacat gcgatatatc aaaatatgga ctttgtagat
      661 acctataatc cagaatatgt attgatatta tctggtgacc atatatacaa gatggattat
      721 agcaaaatgt taaaatttca taaggaaaaa ggctctaaag ctacaatagc agtgattgaa
      781 gttccttggg atgaagcttc aagatttggg ataatgaata caaatgaaga ttcaagtatc
      841 tatgaatttg aagaaaaacc aagtgaacct aagagcaatt tggcatctat gggagtatac
      901 atatttgatt ggaaaatgtt gagaaattat tttaaggaag cagaaaaaaa tccagaaata
      961 aattatgatg attttggaaa aaatttaata cctaaaatgt tagaagataa tgttggaatg
     1021 tatgcttatc catttaaagg atactggaga gatgtaggaa ctatacaaag tctatgggat
     1081 gctaatatgg atataataaa atctccagaa acacttgatt tagcagaccc taaatggaag
     1141 atatatacaa atactatggc aatgcctcca caatatatag gtaaaaatgc aaatgttcat
     1201 agatcaatga tagctgatgg ttgtagaatt ttaggtgaag taggtaactc tgtattatca
     1261 catggagttg ttgttggtaa aggaagtaaa gtaattgatt cagtaataat gccaaatgtt
     1321 gtaataggtg aaaatgtaac tatagaaaaa gctatgatag gtgagtgtgc aactataaat
     1381 gataatgttc agataaagaa tgtaaataat gaaataaatg tagtttctga gtatgaaaat
     1441 atcgaaccta gatgcgtatt aatagaagga ggtctttaag atgagaaatg aatgtttagg
     1501 gataataaat ttaaacaaaa aaggagaccc agcaataaac aaacttaact atggaagacc
     1561 aatagcatct acaccaatag ctggaagata tagaataata gactttgctt tatctaatat
     1621 gataaattca ggaatcacta aagttggaat attcgcaaaa gaaaaataca gatcattaac
     1681 agaccatata ggtagtggta aagattggga tttaagtaga aaaaaaggtg gcctatcaat
     1741 ttttagtcca gaaaacacta agtatagaaa tacttactca catagagaag gtgatatata
     1801 tactatctta gctaatctag attatataga aaagagtgaa gaagaatata ttttaatagc
     1861 tccaagctat atgctatgca atttaaatta ttctcaagca ttggaatacc ataaaaaatc
     1921 tcataatgat ataactataa tatataaaaa tgtaaataat gcaaataaag attttgctgg
     1981 taacttaact ctaaatttag atagtaacaa tagggtaata aatgtaggaa ataatttagg
     2041 taagttccct agagcaaaca tatgtatgga gacatatatt atgaagcgtg aagattttgt
     2101 tgaatgtata tataacattg taaataaagg caattattgt tatttagaag aatttattat
     2161 tgaagaagca gaaaacatga aaataggagc ctatgaatat acaggatatc taaaatgtgt
     2221 aaattctgtt gaatcatatt ttgaaatgag caaagattta ttaaatttag aagttgcaga
     2281 tgaattatta tattctgaaa gaaagatatt tacaaaagaa aagaatgaat cacctacaat
     2341 ttatacagat agtgctaaag tagaaaatag ctttatagca tctggttgtt taatagaagg
     2401 tactgtaaaa gatagtatca tatttagaaa ggtaaatgtt gaaaaaggaa cagttataga
     2461 gaactcaata gtaatgcaaa attgtgtaat aaagtcaaat gcaaaactat ataatgcaat
     2521 attagataaa aatacgagtg tatcaaatgg gaaagaatta agaggagatg aaaaatatcc
     2581 tcttgtagtg gaaaagaata caaatatata aataaatttt ttagaagggg gaatgactaa
     2641 tgaaggtttt ttatgtaaca gcagagtgtt ggccctttgc aaaaactgga ggattaggag
     2701 atgtttctta tgcattgcct aaagaattaa aaaaagaagg tgtagatgta agggtaataa
     2761 tgcccaaata ttcaactata ccaagttatt taaaagatca actaaaagaa atagctgtgt
     2821 ttagtgtacg tgttggatgg agaaatcaat actgtggtct tttagaaatg gaattggatg
     2881 gtgtaaaatt ttactttata gataatgaat tttattttag aagagaagat gaaagaaaga
     2941 gtatctatgg atatggagat gatgcagaaa gatatacttt ctttacagat gcagttttag
     3001 aagctataag tagaattgat ttttatccag atgtaataca tataaatgat tggcatacag
     3061 ggatgttacc attaattcta aaagaaagat atgcaaccct tgaggggtat aaaaatataa
     3121 aaactatgta tactatacat aatctccaat atcaaggtgt atttgataag catgttttat
     3181 atgatatatt ggacttacca caaaaatact ttgataatgg agatatagag tattatggct
     3241 caataaattt tatgaaagct ggtataaatt ttgcagataa aattataact gttagtccta
     3301 cttatgcaaa tgaaatacaa acatcttttt atggcgaaca attagatgga ttacttagaa
     3361 aagagtcagg aaaattaaaa ggtatattaa atggtataga ttatgattta aatgaccctg
     3421 caaaagataa agatatattt gttcattatg atgtagactc aattgacaaa aaagtagaaa
     3481 ataaattgag attacaagat atattgggac ttaaaaaaga ttcatctatt ccgttgatag
     3541 gtatagtttc gagattagtt tctcaaaaag gatttgattt aatagcatac atgatgccag
     3601 aacttatgag agaagattta cagattgtcg ttttaggaac tggagaacat caatatcaat
     3661 caatgttcaa ctattacgat tctaattttt cagataaagt atcagctaga attactttta
     3721 atgcatcatt ggctcaacag atatatgctg caagtgatat gttcttaatg ccatcattat
     3781 ttgagccttg tggtataggt caaatgttag ctatgagata tggttcttta ccaatagtta
     3841 gagagactgg agggcttaga gatacagtaa caccttataa taaatttact ggagaaggca
     3901 atggatttag ttttaaaaat tacaatgctc atgagatgtt tttctgtttg aaaaatgcaa
     3961 tcaaagtatt taaggacaaa gaaaaatgga ttaaattagt tgaaaatgca atgaaaacag
     4021 ataatagttg gaaaaaatca gctaaggagt atatagaaac atatagagac atatgtgatt
     4081 aaaggagtgg tttttttggt aacaataagc aaaaagaaat tcaagcaaaa atttgaagta
     4141 aaaatgtata gcttatatgc acaatctatc aaggaagcta ctgatgaaca attgttaaat
     4201 gtattatgta gtctattgaa agatgagata gcaaaaaaat gggttgctac aaagttagat
     4261 aagaaaaaag aagtttatta ttttagttta gaatttttaa tagggagaca gttaaaatct
     4321 aatttattaa atttaaatat tgaagaagaa gttagagaag gtttatctga attaggaatc
     4381 aacttggatg atttaattga agctgaagta gacccagcct taggaaatgg tggacttggt
     4441 agattagcag cttgcttttt agattctatg gcatctttga acataagtgg tcaaggatat
     4501 ggaataaggt ataaatatgg tctgtttgaa caaaaatttg taaatggtta tcaggtagaa
     4561 gtgcctgata actggcttac agaaggacga tatgcatggg aaactgtaag accaaatgaa
     4621 gcaacaatgg tgaagtttgg tggtgaggta gaattaatca aggaaggtag tcatttaaaa
     4681 gtaattcata aaaactacct tcctgtaatg gcaatgcctt atgatatacc aataatcgga
     4741 taccaaaacc aatgtataaa tactttaaga ttgtttaaga gtgaaatacc aaagagagat
     4801 tttggtgaac ttacttcaaa tgctctaaat tattctggta gctatgaaga ggctttaaaa
     4861 cataagtatt atactgagga aatatcacag gtattatatc ctgatgattc aaattatgca
     4921 ggtaagttgt tgaggttgaa acaagaatac ttttttgtaa gtgcagggat acaagatata
     4981 ataagaaaat ataaaaagaa caaattaaat attaataatt tattcgataa ggtagcaatt
     5041 catataaatg atacacatcc taccttatgc ataccagaac ttatgagaat tttgcttgat
     5101 gaagaaaatt tatcttggga tgaagcatgg gaaataacta aaaaaacagt gtcctatact
     5161 aatcatacta taatgtcaga agcaatggaa aaatggcctg taagtatgat taaagaacta
     5221 ttaccaagaa tttatatgat aatagaagaa ataaatagaa gatatgtaga ggaattaaat
     5281 aataaaggat atgagcagga tagaataaaa agaatgagta taatagactg tgacaatatc
     5341 aatatggcaa acttatgtat tgtcacaagt catagtgtaa atggagttgc taagcttcat
     5401 actcatattc ttgaaacaga ggtattaaaa gatttctatc aagatgaacc aaataaattt
     5461 aataataaga ctaatggaat tgcacatagg cgatggctta taagttcaaa tcctcaactt
     5521 agcaatttaa ttacagattt aattggtgat tcatggaaga aagatacatt acaattaaaa
     5581 aatattgaaa aatttaaaaa tgattcctct gttttacaaa gacttgatga tataaaatac
     5641 aataataaag caaatctagc aaagtttata aaagataaat acgatttaaa tgtagaccca
     5701 agttcgatat ttgatgttca agttaagaga ttgcatgcgt ataaaagaca gttactgaat
     5761 atatttaatg tacttcatat gtatcatgaa ttacttgata atcctaattt gaatcttgac
     5821 ccaagaactt ttatatttgg cgcaaaagca gcaccaggat attatttggc taaatgtata
     5881 attaaattta taaattctgt tgcaagtact ataaataatg atgttagagt aaaggacaag
     5941 ttaaaagtag tatttttaga gaattatgga gtatcattag cagaaataat aataccagca
     6001 gcaaatgtta gtgagcaaat ttcaactaca actaaggaag catctggaac aagtaatatg
     6061 aagtttatga tgaatggtgc tataactttg gctacacttg acggtgcaaa tgtagagata
     6121 tgtgaacagg ttggtaaaga aaatatgttt ttatttggac ttagtgcaga acaagtacta
     6181 aattataata aatatggtgg atattcttca cttgacctat atcattctaa tatggatata
     6241 aaaagggtag tagatgattt aataaatgga tttattccta atcttggcga agaaggaaga
     6301 agtatttata attcactcac tacttacaat gatgagtatt ttgtattgag agattttgaa
     6361 aattatggtc aagctcaagc agatataaac aggttatata gagataaaga aaaatggaat
     6421 aaaatgtctt tagttaatat agctaactct ggattttttt cttctgatag aacaatttct
     6481 gagtatgcaa aagacatttg gtttaaaagg gtgtgattaa atgcaaagtg taatagaata
     6541 taactcttgg gataaaaatt ttaaggctcc gtttggagct ttaaaatttg atgaggaatt
     6601 gaccatttgt gttaaagtaa atgagggata caatatcaag tctatttccc tagaaataaa
     6661 tagagaagaa gaaatgagaa caataacttt aaatgaagaa ttagataatg ataaacttgg
     6721 aaagtgcttt tgtggcaaga ttgaaaagtt tgatggtaca ggtgtttatt tttattactt
     6781 taaagttgat gttgaaatgg atggtcagat aaagacttta ttttatggaa aaaacagaga
     6841 taatggatat tcttgtgaat ataattatag tgatattaat aaatatcaaa tcactgttta
     6901 taaagatttt aaagtaccaa catggtacaa agaaggaata ctttatcata tatttgtaga
     6961 tagattcaat aatggaaatc gtagtggtaa ggttgataat cctaaaaaaa atagtttcat
     7021 atatggaaat tgggaagata cacctatgta cataaaggat agtcaagggg atgttataag
     7081 atgggatttc catggtggaa atttaagagg gataatcaat aaacttggat atttaaaaaa
     7141 attaggtgta agtatactat atctaagccc tatatttgaa gcctctagta atcataagta
     7201 tgatacaggt gactataaaa aaattgaccc aatgtttggg gatgaagata catttaaaga
     7261 acttattgat aaagcaaaag aaaaaggtat atcaatagta ttagatggag tttttagtca
     7321 tactggagca gatagtaaat attttaatat gtatggaaac tataatagtt taggggcata
     7381 tcaatctaaa gaatctccat attattcatg gtatatgttt gaagaatttc ctcaaaaata
     7441 taagagttgg tgggatgtta agacattgcc aaatataaat gaattagaac atagctatat
     7501 ggattatata atatatgaca atgatagcgt tataaataaa tgggtaaata tgggtattaa
     7561 gggctggaga ctagatgttg cagacgaatt accaactaag tttataagag agttaaaaaa
     7621 ggaattaaaa aaggcagatg atgactcaat tcttataggt gaagtatggg aagatgcatc
     7681 aaataagatt agctatggtc agagaagaag ttatttatta ggtgaagaat tagattctgt
     7741 tatgggttat ccttttagaa ataacatgtt ttctttctta aaaggagaaa ttaattcata
     7801 tgaactttgt aatagatata tgcagattaa agaaaattat ccaaaagaat catttaggtc
     7861 taatttaaat ttaattggaa cacatgatgt tacaagagct aaaacggaat taaatgatga
     7921 tgtagattta gtgaagcttg ctgtagctat acagatgaca tttgaaggag taccatatat
     7981 atattatgga gatgaagcag gtctttgtgg aggtgttgac ccagataata gaagaagtta
     8041 tccatggaaa aatgaagacg aagatatgct taatttttat agagatgtta taaaaattag
     8101 aaataagaat aaacttttaa gttctggaaa tacagaattt atatatacta ataatgattc
     8161 tgtattttct tttattagag ttaatgaaaa taatgataga atgttaattt tagtaaatag
     8221 aagtgaaaat gtggaaagta tatcattatg tatagagagt agttttattg aggaaatacc
     8281 tataaagtat agtcttaaaa atgcaaatag tactatacaa attgaaaata atgagttgaa
     8341 agtagattta gactctaaat catttagaat ttttagagta aattaaatat ctttttttag
     8401 aaaaataaat agaaaataag gtgaatgtgg aaagagtttt gttttatcaa aactttttct
     8461 actttttttg tataaattat cataagaatt ttagtatttc aaaatattac aaagaagtat
     8521 aaaaaatatc ataattgtga tattatataa attagtaaga taaaactgaa ctctctacca
     8581 aagttgtagg gagatttaac ttttataatc ttaatatgga ggaacgtctt atgaaggtag
     8641 atccacttac aactcaagta tatgactata tatcaaagaa aatccaaaat ggagagtatg
     8701 aagctaatca gagaattact gaatctgaga tttgtaaatc tatcggagtt agtagaacac
     8761 cagcgagaga agcattaact aggttagcag gtgaaaattt actagaaaaa atacctaata
     8821 agggatttgt agtaaaagaa tttcaggaaa aggagaaatt agacacctat tccgtaattg
     8881 gagtattgga tgcgttagcc ggttcttcag cattacaaaa tttaactgaa tcagacctag
     8941 taaaaatgga agaattaacg gaaatgatgg cggtctctat aaagtataaa aattacaata
     9001 gctatttaaa attaagtaat gaatttcacg atatatacat aactaaatca gataatcagg
     9061 tgttagtaaa tctattgaac tctttaagat ataattttat gtcaaagtca tatactagtg
     9121 ataatgaaga tgagttatat aaaatgttga catacagcaa taatcaacat attgaagtag
     9181 taaaatctat gagggagaat gatttagaaa atgttgaaag agttttgaga gaacattgga
     9241 aaacgatacc tatagcagag atgaaaaaag agaagctttt ataaagtttc tctttatttt
     9301 ttataccatt ttgtcactat ttttttgaat tattaagtag aaaataataa ttaatttaaa
     9361 cttaaggagg aaaactaata tgtatccaaa taaagaaatt gcaatagaag agttagaagt
     9421 ggctggcaaa atgaatcccg gtccatggat aaaacattct tacaatgttg ctaatgtagc
     9481 taaattgatt gcagagcaat gtgaaaattt gaatagtgaa aaggcctttg tgtgtggatt
     9541 attacatgac attggaagaa gaactggtat ttcttctgta aggcatatta ttgatggata
     9601 tgattatgtt atttctaagg gatgggatga agtggcaagg gtatgtttga cacattcttt
     9661 tcctgttaag aatatagaag ctgatattgg aaataaggat attagtaaag aacagtatga
     9721 ttttatcaat cactatttaa acaaccttga atataacgat tatgataagt tgattattct
     9781 ttgtgatgca ttagcagatg caaatggctt ttgtatttta gaaaaaagat ttgttgatac
     9841 aacacgaaga tatggaatat atccgttttc agttgaacga tggaataaga cttatgagta
     9901 taaagaatat ttcgagaaaa taatcaacaa gtctatatat acattgttac ccaatattga
     9961 aaaatctata tattattaaa tcctaagtta ataacttaat atccagtatc accagcagtt
    10021 ggtcattatg attgactgct tttattctgc ttatattaaa aaaatacact atggtttgtg
    10081 tatatcaaag ttatatggaa tgaaaagaga atttatttta tcaattaaaa aatttaataa
    10141 attttatatt aaaattaaaa tacaaaaata ggaaaattat ttagtccaac taaaccaaga
    10201 attttaatat gtgaaataag aataatataa aagtttagta ataaaatact tataaattaa
    10261 aaatagtaaa ctcaaaagct tactaaaact caaaaaagta aaaaaaccaa aataataaaa
    10321 aataagaaag tttataaata ctaagtttta aaaaaattaa tataaacaac aaaaaaataa
    10381 aaagtttata aatattgata tagctaatgt atatgttttt gagtaacttc aaaaaaacac
    10441 ttgtaaaaat gctgagatgt taatataata aatatcatga gtttaataaa agtaaaaata
    10501 aaaaatagaa ataataaata ataaataata aatattaaaa atatttgaat atagagagga
    10561 gatgaatttg aaatcatgga tagcgagttt gcaaatgcag ttgaattaag ctatgagtgt
    10621 gctccattat tagagtcgtt aaaggaatac tcagagaaag atatagcttg ttttgatgtg
    10681 cctggacatg taaaaaatag aggtgtagct atattaaata aatatctagg tgaaagtctc
    10741 atgaaaatgg atataaattc atcaccaact atggataatg tatctgcgcc aaatgggatt
    10801 ataaaaaatg cacaagattt gctagcacaa gcatatatgg ctgatgaggc attctttata
    10861 acgaatggca caactcaagc aatacatgca atgattttga gtgttataaa accaggtgaa
    10921 aaagtattac ttccaagaaa tatacataaa tcagtaataa atgcgttaat actttgtgga
    10981 ggaataccaa tatttataca accagaattt gatgaaaaac taggtataag tttaaatatt
    11041 actcttgaaa aagttagaac agagatagaa aaagattgta atataaaagc attatttttc
    11101 cttaatccaa cttactatgg agtttgtgca gaccttgaga gtattataaa actatgccat
    11161 aaaaataatg ttttagtgtt agttgatgaa gctcatggtg cacattttcc atttcattta
    11221 gatttacctc cttctgcaat aagtttggga gctgacatgg tagctgtaag tatacataaa
    11281 actggaggag cattgacaca atcttcagca ctcttattga atagagataa tgtaagtttt
    11341 gaaaaggttc ttcaatcaat caatatgcta caatcaacat cagcatctta tttgcttatg
    11401 gcaagtatag atggtgctag agttaatcta gttgagaatg gtgaaaagca attgtcaaaa
    11461 gcattaaatc tatctaggta tgcaaaatct aaattgaaca aaattgatgg aataaaagtt
    11521 ttatctacag aaatattgaa acaaaaaggt gttaaattta tagatgaaac aaaattgtgt
    11581 ataaatgtaa aagaattaaa tttgactgga tttgaagtat acgatttatt atataaaaat
    11641 ttttcaattc aagtagaatt gggagattca tataatatac tggcacttgt gtccattgga
    11701 acaaataaat cagatataga tagattagta aaagcacttt ctataattgc aaagatttat
    11761 agaaaagaat caacattaaa tgaatttaat atggttcaaa taaatccaat tattaaactt
    11821 aatccaagag aagcttttta tgcaccaaag gaaagtgtag aaatcaattc atgtatagat
    11881 agaatttgtg gagaatcaat aatggcatat ccaccaggta taccgattat tgctccagga
    11941 gaattgataa cagaagaaat tatggagtat attattttct taaaaaatag caatgcgtat
    12001 cttacagatg tacaagataa aaacttagat agaatactag taataaaata aattacttta
    12061 ttttgcaagg gcaagtgttt gttaagtaga gtttatcaaa tagaacttat taaaagtata
    12121 aaggagttgg ggttgaaatg aaatttgaaa cactaggaag acatatacta gtagaatact
    12181 ataattgtga tgaaaacgta cttaaagacc cacgtcttat agaaaatttt atgaatgatt
    12241 cagcattaaa tgcaaaagcg acaattgtgg attctgtatt ccaccatttt aatccttggg
    12301 gggtttcagg agctgttata attgctgaat ctcatttgac aatacataca tggcctgaat
    12361 atggttatgc agcagcggat ttttttactt gtggtgatat agacccatgg aaaagttttg
    12421 aattattaga gcaactttta aagtctgaaa gaagcgagtc tacagaaatt ccaagaggct
    12481 taactacaaa aattaaaaaa tattcaaaaa aagatttagg taagattact cataaaccag
    12541 aagcagtata aaacatacaa gagaaagtga ggattattat atggaacttt ggtatacaga
    12601 agaatggaca gaaaatgtaa gattttctat aaaagtaaat aaacatctat ttgaaggaaa
    12661 gtctcaattt caaaggatag atgtgtttga tagtgatgaa tttggaaaat ttctaacaat
    12721 agatggatta atgatggtaa catataaaga tgaatttata taccatgaga tgataactca
    12781 tgttcctatg gctacaaatt taaacataaa gaaagtatta gtcataggtg gaggtgatgg
    12841 aggtgcagta agagaattaa gccgttatcc tcaaatagaa aagattgaca tggttgaaat
    12901 agataaaatg gttgtagatg tatctaaaga gtatatggat atatgctcat gtaaattaga
    12961 tgataaaaga gtgagtttat attttgaaga tggtgttaat tttgttaagt gtgcacatga
    13021 taaatcatat gacctgataa tagtagattc aactgaccca ataggaccag gtgaagggct
    13081 tttctcaact gatttttata aggattgtta tagaatactg actgatgatg gaattttagt
    13141 aaatcaaagt gaaagtccgt attttgactt taatgctaaa gagatgaaaa gagctaataa
    13201 aaaattaaaa cagatatttc caatatcaga agtttatcaa gcacatatac caacatatcc
    13261 atctggtcac tggttgtttg gatttgcttc aaaaaaactt aatccagtaa aaaatcaaga
    13321 tagaaatgga tgggagaaat taagtttaaa aactaaatat tataatagtg atatacattt
    13381 aggttcgttt atgttacctc aatacgtgaa ggagatgcta gatgaagaat aatttctatc
    13441 atatgaacac ttttatgagt atggacaaaa attatgaaga atctaatctt atagtatttg
    13501 gtgttggatt tgatggaact acttctaaca gacctggggc tagatttgca agtagctcta
    13561 tgagaaaaga attttacggt cttgagacat atagtccttt tttagatttg gatttagagg
    13621 attataatat atgtgattat ggagatttag aaattagcgt tggaagtaca gaacaagtct
    13681 taaaagaaat ctatcaagag acatataaga ttgttagaga ttcaaaggta ccctttatga
    13741 ttggaggaga gcatttagtt acattaccag cctttaaagc agtacatgaa aagtacaatg
    13801 atatatatgt aattcatttt gatgcccata ctgatttgag ggaagaatat aataatagta
    13861 aaaattctca tgcaacagta attaaaagaa tatgggatat tgtaggtgat aataaaatat
    13921 ttcaatttgg tataaggtct gggacaaaag aagaatttaa atttgctaca gaagaaaaac
    13981 acacatacat ggaaatagga ggaatagata cgtttgaaaa tatagttaat atactaaatg
    14041 gaaagaatat ttatctaact atagatttag atgtattgga tgcatctgtg tttccaggaa
    14101 caggtacacc agaacctggt ggtgtaaatt atagagagtt tcaagagatt tttaagatta
    14161 taaaaaactc taatataaat atagttggtt gtgacattgt agagttaagc cctgattacg
    14221 atacaacagg tgtatcgaca gttatagctt gtaaaatctt aagagagtta tgcttaataa
    14281 tatctgataa aattaaatag agtattttat ataaatagta tatattataa tataagaagt
    14341 tattttaaaa aagttagttt aagtttgacg cttttctaaa gtaacttctt atttttgtaa
    14401 ttaaaaataa atataataaa caacaaaaaa gagccctcga tagctccttt tctaaaatat
    14461 atatatttaa attaaataat tatattctat ttacgttttc agcttgaggt cctctattac
    14521 cttgagttat atcaaagcta accttttcgc cttcttctaa agttttgtat ccatcatttt
    14581 gtatagctga gaaatgtacg aaaacatcat cttctccctc tacagatatg aatccaaatc
    14641 ctttttcatt gttaaaccat tttactattc cgtttttcat tataagaaaa cctccgcatt
    14701 attaaaaaat acaaactaaa aaaataagtc tgttctatta atataacata aattttaaat
    14761 aaaagcaaat aaaattttta aaaaaattaa ttacttttag aatttaataa aaattg
//
LOCUS       DAEMHW010000086        14800 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEMHW010000086 DAEMHW010000000
VERSION     DAEMHW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.086"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            199..951
                     /locus_tag="KPK67_002197"
     CDS             199..951
                     /locus_tag="KPK67_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2459162.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            1053..1973
                     /locus_tag="KPK67_002198"
     CDS             1053..1973
                     /locus_tag="KPK67_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBG2459163.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            1976..3892
                     /locus_tag="KPK67_002199"
     CDS             1976..3892
                     /locus_tag="KPK67_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBG2459164.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVHVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGVVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            3912..4781
                     /gene="gatY"
                     /locus_tag="KPK67_002200"
     CDS             3912..4781
                     /gene="gatY"
                     /locus_tag="KPK67_002200"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG2459165.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKNVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            5291..6754
                     /locus_tag="KPK67_002201"
     CDS             5291..6754
                     /locus_tag="KPK67_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBG2459166.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIVDGLEELERMLEV"
     gene            6758..7993
                     /locus_tag="KPK67_002202"
     CDS             6758..7993
                     /locus_tag="KPK67_002202"
                     /inference="COORDINATES: protein motif:HMM:NF013579.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009172622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBG2459167.1"
                     /translation="MEYIKLREVCDLKNGFAFKSSDYIEASNVLNCRMSNIRPNANFD
                     AEYNPKYLPEEFSEKYKEYLLKDGDLIIAMTDLANDPKLLGVPTIVDTKGYKMLLNQR
                     VGKLILLDKSKVLESYLKYTLSLPQNKNYFKKFASGGLQINIGKEDILNLEIPYCDVR
                     TQEQIVKVLDKAQELIDKRKEQIEALDELVKSRFIEIFGNPVNNPRGWEVKRLNDIST
                     EILSGTTPKGGSQVYIEKGIIFLRSQNVWKNKLVLDDVAYIDEETHNSMKKSSLKNRD
                     ILITKTGRINTENSSLGRAAMFLGEDDSANINGHVYLIRLKKNIINEFVLFILTIDVY
                     REYIRSVCVGGIDKRQINKEHLEEFPIILPPVELQNEFVGFIKEVDKLKLKIEDSLKQ
                     LEDNFNSLMQKAFKGELFN"
     gene            8026..8334
                     /locus_tag="KPK67_002203"
     CDS             8026..8334
                     /locus_tag="KPK67_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459168.1"
                     /translation="MNQIELDLYHNMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            8367..8747
                     /locus_tag="KPK67_002204"
     CDS             8367..8747
                     /locus_tag="KPK67_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBG2459169.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            8737..9165
                     /locus_tag="KPK67_002205"
     CDS             8737..9165
                     /locus_tag="KPK67_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459170.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELRAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDHLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            9215..12481
                     /locus_tag="KPK67_002206"
     CDS             9215..12481
                     /locus_tag="KPK67_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG2459171.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKNSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPKMMNAIDDTK
                     SKDGKRLFTPGHFDSYLMGLTATPKDEIDKNTYSVFDMENGVPTYAYEYNKAVEDGYL
                     VDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFNDDESIGDEIGNNAVNEWLFNSDT
                     IDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHARAIVERFNKLYPKYGGNFVKAVDY
                     SINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIPEILNLVFFKKVRSKTKFWQMIGR
                     GTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPKGFEGNKAETLTEKLFNIKVSMVK
                     ELQDIKYIEGEYAELRKELLEELITSVKALNEDSYIVRMNLSYVHKYKNENVWSNIGA
                     VAQNEIREYISPLITSYSDDELAKRFDVVMYNIQLAYLQNNNASKGIRHVMATAEKLS
                     KLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEVRISLRELIKYLEKSSQKIYYTSF
                     EDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKDELAIFKLRNNKKITKQDVETLEE
                     ILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREAANEAFSEFLNNKSFNTKQIHFVK
                     LIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFENHIEERNKLIKTINKIKENASEIG
                     "
     gene            12862..>14800
                     /locus_tag="KPK67_002207"
     CDS             12862..>14800
                     /locus_tag="KPK67_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBG2459172.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNTTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKG"
BASE COUNT         5963 a         1431 c         2816 g         4590 t
ORIGIN      
        1 gaggtacaat tcttttttat tttaaaatgc tataagataa cataataaac tttaagcaaa
       61 aaattatgat attataaatt tacatatata gacattgact aaatcttaat tgtatataaa
      121 aataagtagt taagaggtaa tatattatat tactcgataa atttagacaa ataaaactta
      181 tataagggga gataacatat gataattgag ctaaacaaaa gtaattctga aaatctaacg
      241 aagacggaac ttgaaataat taaatttata aatgaaaatg aatcaatatt accagaatta
      301 tctatagtag agattgctca tgaaacatat tcatctccag caaccgtttc aagagcaatt
      361 agaaaatgtg gtttaaatgg attcaatgaa cttaggtata gattaacagt aaaagatgaa
      421 aatgatgata tacaaaatat gggggatata ataaataaat cctttataga agcacagaga
      481 gttatagaac aaatatcttt aactacacta atcaatataa taaaaactat aaataaatct
      541 agtaagatat atattttagc aagagggctt actgaatatg tagcaaaaga gtttagtctt
      601 aaattgcaat tacttgattt taatgttgtt tttataaatg accccaacat aatgaaaata
      661 aaatctaaat caataaaaaa agacgaactt ataattatat tttcattgaa tggaaatacc
      721 atagagcttg tagaatcagc acagaatgcc aatttagttg gagctaaggt tataacttgc
      781 tgttgtagtg aaagtgcaaa acttataaag tattcggatt tatatattgt aggatacaaa
      841 catgaacata tatcaatttc tgaatatgaa gtatcttcca gattgccctt gtatattata
      901 tctaggataa ttatagatta tatggtcaga tataataatt ctgaaaatta aattgaaaag
      961 tctatgaaaa tcgattttct atttgtgaaa agattttcat agactttttt attataatga
     1021 aaacatacac agcacaagca agggggaaga caatgatata tacattaact acgaatcctg
     1081 caatagatat gaatatatct actaatggga taaagagaaa attagtaaat cgtacatcaa
     1141 atgctgtata ttctcctaat ggaaagggat taaatgtaac ttttgtacta ggtcattatg
     1201 gtatagaatc taagatatta gggttttttg gaggattttc aggaaaatat atagttgaag
     1261 aaagcgaaaa gagaggattt gacgtattgc ctacttgggt agaagataca actcgtataa
     1321 atatattttt aaatgatgga agtgatgaat ttaaatttgt aaactctggg tcatatgtaa
     1381 acgaaaaaca aaaactagat atgctagata agatacagtc attagcagat ataagttact
     1441 tatcaataag tggaagtcta cctcctggta ttgatgataa ttactatgag gatatattta
     1501 aaatttgtaa aaataaaagt ataaagacaa tactcgatat aagttcacca aaattaaagg
     1561 agttactaca gtataatcca tatttgataa aaccaaatga tgaagaaatc aaagatatat
     1621 ttggaattat tgtaagagat gaagaagata ttaaggatgt acttaaatta ctgcatttaa
     1681 aaggtgctca aaatatattg ctaactttag gagaaaaagg ctcttatttt tataatggaa
     1741 aatcaattta ttatgctagt gctcaaccag ttacagtagt cagctctgca tgtgctggtg
     1801 attctgcatt agctgctttt ttaagtatat ggttggaaaa tccagaacat attgaagaag
     1861 cattaaaaag gtcagcagca acaggagcaa gtgtggctga aagtaatggt ataggtaatt
     1921 tggagaatgt ggaagattat ataaaaaata taagagtcag aaaggtggta taaaaatggg
     1981 aaagaaaatg attctagggg taacaggttg tccaactgga atagctcata cttttatggc
     2041 agaagaagca ttaaaaaatt cagccaggga attaggctgt gacataaaag ttgaaacgaa
     2101 tggagctata ggtgtagaaa ataagttaac agcaaaagat atcgaaatgg cagatgcaat
     2161 aatagttgct tgtgataaaa atgttgatat ggacagattc aatggaaagc cggtaataga
     2221 agtacctgtc aaagaaggta tacataaggc taatgaactt atacaaaggt gtattgatgg
     2281 aaaagtagct attagaaaag gaaacttatc aagcagtaaa attgcagaag aaggaaattt
     2341 atcgtttggt caaaagttgt ataaagactt aatgaatggt gtatctcaca tgttaccttt
     2401 agtagtagca ggtggagtac ttactgctat atcattttta tgggggattt attcatttga
     2461 cccaaattca gaacaatata atcaaatagc agctacatta aaatctgttg gtggatattc
     2521 tatgaacttg atggttcatg tattggcagc atttatagct caatctatat cgggaagagc
     2581 tggtatgttg gcaggacttg ttggaggaat gatatcattt gatacaggtt cggggttttt
     2641 aggtggaatc atatcaggat ttttagcagg ttacttagtt aaactatttg tacatttgtt
     2701 aagaaaatta ccacgacaat tagaaggtct taaatcgatt tttataattc ctattgtcag
     2761 cgtaggtctt gtgggtgtag caatgttgtt acttggtggg ccatgttcag cacttaacaa
     2821 tgctatgatg aactttctat cggggttaca aaattcaagc cctataattt tgggagtagt
     2881 aataggatgt atgtcagcat ttgatatggg aggtccagta aataaagcag catatgtaac
     2941 tggaactatg cttctaggac agggaaatta tttatttatg gcaggtgtat ctgcagcttg
     3001 tataacacca ccattggtaa tagcaatagc atctacttta tttaaaaata gatttacaga
     3061 agaagataga gcagcaggat taatcaatta tatactaggt agtactcata taacagaggg
     3121 ggcaatacct tttgcagcaa aaaatccatt aaaagtgttg ccagtactta tgattgcatc
     3181 atcaatatca tcaattttaa cttatataat gaaaatagaa gtaccagctc cacatggagg
     3241 ttttttggtt ctaggattag tcaataagcc acttttatgg gtagggtgta tatttgtagg
     3301 ttcattagta ggtgctgtac tttatatgat ggttacacct aaagtagtaa atgagaaaaa
     3361 tactatagaa aatacaaatg caacaaaaga tgaacataaa aatgaaagtg cagaatcgga
     3421 aaacgtatct aataatatct ctttatatag cgaagaaaca gtaattttag atgttaaggg
     3481 aaaaaataaa acagatgtaa ttgatgaaat gattggaata cttgataaat ctggtgttct
     3541 tttagataaa aagaagttta aagaagaaat ccttaaaaga gaagaaatat catctacagg
     3601 ttttggtatg gggatagcga taccacatgc aaaaacagat gcagtaaagg taccacgtgt
     3661 agcagtaggt gtatcaaaag aagggtttga ctttgaatca gaagatggaa gcttggcaca
     3721 tattatattt atgatagcag ctacagataa tggtgataat ttacacttaa aaacattatc
     3781 tcaactatct tcaaaattaa tgaatgaaga atttttaaat gagttattaa atagcaaaac
     3841 aagtagagaa atagtatcaa aattaaataa cgaagaaatt aaatcagtat aaacatatag
     3901 gaggagaaat tatggcatta gtgacaacta aacaaatatt attagatgca caagaagggc
     3961 attatgcagt aggtgcattt aatgttgaaa atatggagat ggttatggca gtaatagagg
     4021 ctgctgaaga acttaaatca ccagtaatat tgcaaacaac accttcaaca ataaagtatg
     4081 caggactaga ttattattta gcgaatgtaa aggtagcatc agagaaggca agtgttccag
     4141 tggcaatgca cttagaccat ggttcaagct ttggactagc tatgcaggca cttagagttg
     4201 gatatacttc tattatgatt gatggttctc atgaaagtta tgaagataat atttcaatat
     4261 ctaaatctgt tgtagatgca tgtatgcctt cagatatacc tgttgaagct gaattaggta
     4321 aagttggagg taaggaagac gatttagatg gtggtgatgg gggagcatat acagacccat
     4381 tagaagcaaa agagtttgta gagagaacag gggtatcatc tttagcagta gcaataggta
     4441 cggcacatgg attatataag ggagagccaa agcttgattt agatagatta tctgaaatta
     4501 gagatgttgt atcagttcca ttagtattac atggtgggtc tggaatacca gatgaagtta
     4561 ttaaggaatc tataaaaaga gggatttgca aggttaatta tgcaactgaa ttaaggattg
     4621 cttatagcaa tggagttaag aatgtgctta attctgaccc agaggtaatt gaccctaaga
     4681 aatatggtaa aaaaggttta gagtcagtta aagattttgt taagagtagg atggaagttt
     4741 gtggatgtgt aggaaaagca atagagatag tgaagtgtta ataataaagg gagagaataa
     4801 gtaagattaa attaaggggt gtctcaaaat gaacttttta gttcaggagg cactcttttt
     4861 tgtatgaaat taaatatatt ttcatcattc cgaaaataaa aaagaagatt atatctattt
     4921 tgagacagca tcttatttta ttttttgatt aagattgtaa ttgagaaatt tgaaataagg
     4981 aagatgatat aattaaataa aaaatttctt tctaagttta aattaatatt aaaaagttat
     5041 aatatggtaa gagttatcca ggtagatgac ttctatgctg gatatatgat aaaataattt
     5101 tgtatgggat gtataataat cataaaataa gagtattgca ttagtttttt aactaaaaca
     5161 tgtaaatagg aaaaataatt taataactgt gtagtaaaat tttgaattta atataagaca
     5221 ctaattatta aggcgtacga ctaaaatata tatttaaatt attttatgaa cactggaggg
     5281 aaagtcatat atgattacag gagaattaag gagcaaagta gacaagattt gggagacatt
     5341 ttggactggt ggattaacta atccattaga agttatagaa caatttacat atcttttatt
     5401 tattaaagga ttggatgaag tagaaacaaa aaatgaagca gaagcaactc ttttgggagt
     5461 ttcttttgaa aggatatttg atgatgaaca tcaacattta agatggagta agttttctaa
     5521 tgaaggaaat tcagaaaaaa tgtatgaaat agtacaaaat gaagtgtttc ctttcataaa
     5581 aaaattacat ggaaataaag actcagctta tgcaaagtac atgggagatg caatatttaa
     5641 aattcctacg cctcttatgt tatctaaaat agttgatggt attagtaatt tagaattaag
     5701 taaggataga gatacaaagg gagatttgta tgagtattta ttatccaaag tggctacagc
     5761 aggtactaat ggtcaattta ggactccaag acacataata gatatgatag taagacttat
     5821 taaaccaaca ccagaagata ttatagtaga cccggcagca ggttcagcag gttttttagt
     5881 atcatcacaa caatacttaa gggataatca ttcaagtctt ttcttagtgc aagggctgaa
     5941 ggagcatttt aataatggta tgttctatgg atttgatatg gatagaacta tgcttagaat
     6001 aggtgctatg aatatgatgt tgcatggcgt tgacaatcct aatatagagt ataaggattc
     6061 tttatcggag gttaatacag ataaagataa gtttacattg gtgttagcaa atcctccttt
     6121 taagggaagt ttagattatg aagcagtgtc agcagattta ttaaaagtga ctaagacaaa
     6181 gaaaacagag ttattattct tggcactttt tttaagagtt ttaaaaattg gtggaagatg
     6241 cgcatgtata gtgccagatg gagtattgtt tggttcaaca ggtggacata aatcgataag
     6301 aaaagaaata gttgataatc ataaattgga agctataata tcaatgccaa gtggagtgtt
     6361 taagccttat gcaggagtat ctacagctat aatgatattt actaaaacag gaacaggtgg
     6421 aactgataag gtatggttct atgatatgaa agctgatggt tattctttgg atgataagag
     6481 aaatgagata aaggatagtg atatagagga tattattaag agatttgata atcttgatgg
     6541 tgaagtagat agaaaaagaa cagagcaaag cttttttgtt gatgtggaag agataagaga
     6601 gaatgggtat gatttatcta ttaataagta taaagaggta gtgtatgagg aagtagttta
     6661 tgatgctcct agtgtgatta taggtagagt tagggagtta gagaaggaaa tcgtagatgg
     6721 attagaggaa ttagaaagaa tgcttgaggt gtagagtatg gaatatatta agttaagaga
     6781 agtttgtgat cttaagaatg gttttgcatt caaatcaagt gattatattg aagcttctaa
     6841 cgttttaaat tgtagaatga gtaatattag acctaatgct aattttgatg cagagtataa
     6901 tccaaaatat ttaccagaag aattttctga aaaatataag gagtacttac ttaaagatgg
     6961 agatttaata atagcaatga ctgatttagc taatgaccct aaattattag gagttcctac
     7021 catagttgat actaaagggt ataaaatgtt gcttaatcaa agagtaggaa agcttatact
     7081 attagataaa agtaaagttc tagagtcata tttaaaatat acattaagtc taccacagaa
     7141 taaaaattat tttaagaaat ttgctagtgg agggttacaa ataaatattg gaaaagaaga
     7201 tatactgaac ttagaaattc catattgtga tgttagaact caagagcaga tagtgaaggt
     7261 tttagataaa gctcaagagt taatagataa aagaaaagag caaatagaag cattggatga
     7321 attagttaaa tcaagattta ttgagatatt tggtaatcca gtaaataacc ctagaggatg
     7381 ggaagttaaa agattaaatg atatatcaac agaaatattg agtggaacta ccccaaaggg
     7441 aggaagtcag gtttatattg aaaaaggaat tatcttttta agaagtcaaa atgtttggaa
     7501 aaataaatta gtacttgatg atgtagcata tattgatgaa gaaactcata atagtatgaa
     7561 aaaaagtagc ttgaaaaata gagatatttt aataactaaa actggaagaa taaatactga
     7621 aaatagtagt ttaggtagag cagctatgtt tttgggagaa gatgatagtg ctaatattaa
     7681 tggacatgtt tatcttataa gacttaaaaa aaatattata aatgaatttg ttttatttat
     7741 acttacaata gatgtatata gagaatatat aagaagtgta tgtgttgggg gtattgacaa
     7801 aaggcagata aacaaagaac atttagaaga atttcctata atattaccac cagtagaact
     7861 tcaaaatgaa tttgttgggt ttattaaaga agtagataaa ttaaaattaa aaatagaaga
     7921 tagtttaaaa caactagaag ataactttaa ctcattaatg caaaaagctt ttaaaggaga
     7981 attgttcaac taaaatacaa ttattaaaat gggggaataa aatctatgaa tcaaatagag
     8041 ctagatttat accataacat ggtaaatatc tataaagaag ctgatttaca atgtaactat
     8101 aagccaacaa gatttttaca actgataaat aacaaaggag ccttattagc tgctaaagaa
     8161 ttaatcaata aagaaggagc tactgaagga tttacaaggc tatgggagtg taagagatta
     8221 gatttatcat tagaagtatt agtattaaaa gaaaagtata aagagttgtt tactgatgaa
     8281 gaaagagaaa tatgtataaa tagattaaaa gattatggat ataaattcaa ttaagcaata
     8341 ttacctaata aacaagaggt gattaaatga gaaatatacc aaatgagatt aaaaactcaa
     8401 taataaatgt atttccaaaa gaaaatactc tagcaatcat aatatttggt agttatggaa
     8461 cagataaaca tacatcagaa agtgatatag acatagcttg gattcccatc aaaaaagtac
     8521 caattacaga acttgcaact aagacagaag ctctaagaaa tatattagac attgatgtag
     8581 acctaaaaat tgttacagat aattatacaa tagcacttaa aaaagctatt ttagaaggtg
     8641 aagttattta tgaatctaaa gattttaagg attatataaa taaattctat tttgaaaaca
     8701 gtgatgtaat agatatctta gattggaggg acatgtatgg taattagaaa caaattatct
     8761 tggaactcaa tagaagaagt aaatagggat tttggttctt gcttgtatga cctgcaaaac
     8821 ttcaaaatct tatatgataa ggaagaaatg cctgagttaa gggctagata cataaaagaa
     8881 ggctttaaaa gctatatggt atcatttcta gaattaacaa aagcaatgtt gtattataaa
     8941 tcaattgatt taaacataaa atctaaaaac ttttatgacc accttttagc ttgtgagtat
     9001 cacaatttat tgcctaaaaa ttcttctata gttatagaaa cgcttagaaa attgagaaat
     9061 gatgattctc atggatatga tataccacag tttgaagata tgtacgaatt gtttacagaa
     9121 aatgaagaag tatttgttag tataagaaac agctgtaaga aatagttttt agagagattt
     9181 attttaacta agggacaaaa gggggagaaa agccttgggt aattttgatt ttttacaaaa
     9241 gaagaaaaat ttcaatagtt ttaatactgc ttgcttagaa gcagaaaaaa gtatactagt
     9301 tagcccatct acttgtgcaa ctataacaag aagagcttta gagcttgcag taaaatggct
     9361 atatgctaat gatagtgatt tagtattgcc ttatcaagat aatcttagta cgttgattca
     9421 taacaatagt tttatagaac ttatagacta tgatatgtta cctttattaa aatatatagt
     9481 taaattaggt aatttatctg tacatactag tgcgaacata gagagaggag aagcaatact
     9541 ttcacttaac aatttgcatc aatttgttag ttggatagac tattgttatt cagatgaata
     9601 tacagcagaa gactttaatg aagatttatt acttcatggg gaagagaaaa ggacgagacc
     9661 agacgaactt aaaaatcttt atgagagatt aagttcaaaa gataaaaagc ttgaagaagt
     9721 aataaaagaa aatgaggaat taagaaagtc actaactgaa aagagaaaag ttaatactgt
     9781 aaactatgat ttcaagatag acgaagtaag cgaatttgag acaagaaaaa gatatataaa
     9841 tatagaatta aagcttgcag gttgggaatt tggaaaagat ataggggaag aaatagaagt
     9901 acaaggtatg cctaatgaaa gtggtgtagg atatgtagat tatgttcttt atggtgaaaa
     9961 tggtaagcca ttagcagtag ttgaggcgaa aagaacgagt aaagacccga aaattggtca
    10021 acaacaagct aaattatatg cagattgttt ggaaaagaga tatagtcaaa gaccagtaat
    10081 atttttgact aatggttttg atatgtatat ttgggatgac tattcagata gaaaagttta
    10141 tggattttat aaaaaatcag aattacaact tatgatagat agaagaaaga gtaagaagtc
    10201 tttaagttct gtaacaataa atgatgaaat atccaataga tattatcaaa aagaagctat
    10261 tagggctgta tgtgaggctc ttgaaaataa gcaacgcaaa actcttttgg tatgtgctac
    10321 tggtacagga aaaacaagaa ttgctatatc tatagttgat gtactttcaa gacataattg
    10381 gattaaaaat atattatttc tagcagatag aaaagcattg gtaaagcaag ctaaaaacag
    10441 ttttactaag ctattaccta acttggcgtt atgtaattta ttggatagca aagatagccc
    10501 agaagatgct aggatgatat tttctacata tccaaaaatg atgaatgcaa ttgatgatac
    10561 gaagtctaag gatggaaaga gattgtttac acctggtcat tttgattctt acttaatggg
    10621 tcttactgct acaccaaagg atgagataga taaaaatact tattcagttt ttgacatgga
    10681 aaatggagta cctacatatg cttatgagta taataaggca gtcgaggatg gatatttagt
    10741 agattatact agtattgagt ttaagactaa gattatggaa gatggaatta agtatgatga
    10801 gctttctgat gaagagaagg aagaatatga aaatacattt aatgatgatg agagtattgg
    10861 agacgaaata gggaataatg cagtaaatga gtggctattt aattctgaca caatagattt
    10921 agttttaaat aagctaatga cagaggggtt gaaaatagaa ggggaagaaa agattggtaa
    10981 gactataatt tttgcaaaga atacaaagca tgctagggca atagttgaaa gatttaataa
    11041 actgtatcca aagtatggtg ggaactttgt aaaagcagtt gactatagta taaattatgt
    11101 agattctata atagatgatt tttctgataa gaataaatta cctcaaatag cagtttcagt
    11161 agacatgctt gatacaggaa ttgacatacc tgaaatacta aatcttgttt ttttcaagaa
    11221 agtgagaagt aagactaagt tttggcaaat gataggtaga ggaactagat tatgtccaga
    11281 tttattaggt gttgatatgg ataaggaaag attcttaata tttgattttt gtaataattt
    11341 tgaatttttt aaatttaatc ctaaaggatt tgagggaaat aaagcagaaa cacttactga
    11401 aaaattattt aatataaagg taagtatggt taaagaactt caagatatta aatatataga
    11461 aggtgagtat gcagaactta gaaaagaatt gttagaagaa cttataactt ctgtaaaagc
    11521 tttaaatgag gatagttata tagttaggat gaatttaagt tatgtacata agtataaaaa
    11581 tgaaaatgtt tggagcaata ttggagcagt agcacaaaat gagataagag aatatatatc
    11641 accactcata acttcatata gtgatgatga attggctaaa agatttgatg tagttatgta
    11701 taatatacaa cttgcatact tacaaaataa taatgcaagt aaaggtataa ggcatgtaat
    11761 ggctacagca gaaaaacttt caaaacttgg gactatacca cagattcagc aacaaaaata
    11821 tactattgaa aaagcaatga cagaggattt ttgggaagat tcagatatat ttgaggtaga
    11881 agaagtacgt atatcattaa gagagcttat taagtatctt gaaaagtcta gtcaaaagat
    11941 atattataca agttttgaag atatgattgt agcagaagat agaaatgatt ctgtatataa
    12001 tgcaaacaat cttaagaatt ataaaaagaa ggtagagtat tatttaaata gccataaaga
    12061 tgaattagca atatttaaat taagaaataa taaaaagata acaaagcaag atgttgaaac
    12121 gttggaggaa atactcttaa aacagttagg taattgtgat gattataaga aggagtttgg
    12181 agatactcca gtaagtcaat tagtgagaag actagtggga ttggatagag aagctgcaaa
    12241 tgaagctttt tcagagtttt taaataataa gagctttaat actaagcaaa tacattttgt
    12301 taaacttata gtcgattatg tagtcaagaa tggatttata gaggataaca aagtgttgat
    12361 ggaagaccca tttagaactg ttggaagtat aattgattta tttgaaaatc atattgaaga
    12421 gagaaataaa ttgattaaaa ctataaataa aattaaagaa aatgcatcag agataggcta
    12481 atgatgttat ttagtgcaaa attattgtta atgatgtaga gaataaattt attagtaggt
    12541 agtctaatat tgggttgaaa tagataacta gatatggata atgtaaatat acaaagagag
    12601 gtattgaata attatgcaat atctcttttt aattgataaa taactatgaa ttactcatga
    12661 agaagattaa ataataattt ttaaaattaa gtatttgtat attttataga ataatcatgt
    12721 atttacttta ataaaataaa aacaggtata aaaaggtata taataaggct ttcatataca
    12781 tttatgagtc aaaattttga tataatatat agatacatgg agttatgaat ggaatttatt
    12841 taacttatag ggggagttaa tatgaaagca ggagaaattg aatttcttag ttatttagag
    12901 gggtcaaaca aaagttttgt agtacctgta tatcaaagaa attataactg gaagaaagaa
    12961 caatgtaaaa gattgtttga tgatttagag gatataatag attctggatt tagaactcat
    13021 ttcttagggt cagttgtatc tatatatagt atgggaaaag aatatttaat aatagatgga
    13081 caacaaagag tgactactgt gtcaatatta ttgattgcca tgtgtcaaat aataaaagag
    13141 tttggggaat caaaaggaat tattacaaca gaagaacaga ttaaggagtg ttatcttata
    13201 aacaagcact caagagataa agatggaaaa ataaagttaa aaccaatagc agaagataga
    13261 aaagcgtact atgaactatt tgaagaagaa atgaatacag atagaaattc taatatagtt
    13321 tcaaattatt tatattttta taatagaata aaagaagata atatatcaat agatgatttg
    13381 tttgatgcta tacagaaatt aactatagta gaaatagagt taaaaaatgg tgaagatgac
    13441 cctcaattaa tctttgaaag tctaaattca actggcttag atttaagtga agctgataga
    13501 gtaagaaact ttgtgttaat gggtagaagc tctaagttac aagaggaatt ttataataat
    13561 tattggtata aaattgaaaa aaatacaagt agcaacgtaa gtagttttat aagagactat
    13621 ttgactataa aggagagaag tataccgaat aaaaataaag tatattttag ttttaaaagg
    13681 tatgtatcag ataatggtat agatatagaa attttattaa aagatttact taaattttct
    13741 aactattata gagagatatc ttattcatgt actgatgata atgatgtaaa caaagttttg
    13801 aaaaggataa acaaacttga aatatttgta tcatatccgt ttatattaga attatttgat
    13861 gattataatg aaggtataat aaataaaaaa gtacttgtag aatcccttaa tttagtagaa
    13921 tgttttatat taagaagaat agtatgtgat gtacctccga atgcactgaa taaagtattt
    13981 atgaacttag gaagggaaat taaaaaattt agtgattata aagaaaacta ttctaatata
    14041 ttaagatata tttttataaa taagaaatca tctcaaagat ttcctactga cagtgaattt
    14101 tcaaatgctt ttgaaactaa agatatatat aatactaagt caaagaataa gttatatata
    14161 cttgagaggt tagagaatta taataataaa gaaattgttg atttagaaaa tttaatcaat
    14221 aataatatgc taaatataga gcatataatg ccacagacat taacaaataa atggaaggaa
    14281 tcactaggag ataactataa ggagatacat gataaatatt tacatacaat tggaaattta
    14341 actttgacag gatataattc aaaactaagt aataaaacat ttgaagaaaa gaaagaaatg
    14401 gaagatggat ttaaagatag taggctttat ctaaataaat atatttcatc tatagataaa
    14461 tggaatgaag aagaaataaa aaatacaact aagatattac tagatagagc tgtagaaatt
    14521 tggaaatatc caagtgttac atatgaacct ccaagtaaca gtgaaaatat atttacgtta
    14581 gaggatgatg atgttaattt tacaaatgaa agtatagtat catttagtat attaggagag
    14641 tcatataagg ctaaaaattg gacagaattt tatgaacaag ttgctcttac tttatatgat
    14701 ttggattctg taaggtttaa cagtattgta gacaaaactt ataaaagaga ttttatagat
    14761 aatattaagt ctagagatgc atataaatta agaaaaggta
//
LOCUS       DAEMHW010000087        14752 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEMHW010000087 DAEMHW010000000
VERSION     DAEMHW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.087"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            379..570
                     /locus_tag="KPK67_002208"
     CDS             379..570
                     /locus_tag="KPK67_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459173.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(659..1471)
                     /locus_tag="KPK67_002209"
     CDS             complement(659..1471)
                     /locus_tag="KPK67_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBG2459174.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGLKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            1893..3044
                     /locus_tag="KPK67_002210"
     CDS             1893..3044
                     /locus_tag="KPK67_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2459175.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFELEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVNFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            3162..3800
                     /locus_tag="KPK67_002211"
     CDS             3162..3800
                     /locus_tag="KPK67_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG2459176.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            3801..4823
                     /locus_tag="KPK67_002212"
     CDS             3801..4823
                     /locus_tag="KPK67_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBG2459177.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            4813..5433
                     /locus_tag="KPK67_002213"
     CDS             4813..5433
                     /locus_tag="KPK67_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459178.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVYLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(5557..6243)
                     /locus_tag="KPK67_002214"
     CDS             complement(5557..6243)
                     /locus_tag="KPK67_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBG2459179.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDVLGGINSGIDTCWLNSNNSINSTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(6257..6802)
                     /locus_tag="KPK67_002215"
     CDS             complement(6257..6802)
                     /locus_tag="KPK67_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2459180.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMADDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            7030..8277
                     /locus_tag="KPK67_002216"
     CDS             7030..8277
                     /locus_tag="KPK67_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG2459181.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPIIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            8364..10357
                     /locus_tag="KPK67_002217"
                     /pseudo
     CDS             8364..10357
                     /locus_tag="KPK67_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
     gene            10357..11679
                     /locus_tag="KPK67_002218"
     CDS             10357..11679
                     /locus_tag="KPK67_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG2459182.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            11958..13244
                     /locus_tag="KPK67_002219"
     CDS             11958..13244
                     /locus_tag="KPK67_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG2459183.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGTINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            13330..14637
                     /gene="grdB"
                     /locus_tag="KPK67_002220"
     CDS             13330..14637
                     /gene="grdB"
                     /locus_tag="KPK67_002220"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:14377..14379,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG2459184.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     DEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGMAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
BASE COUNT         5892 a         1548 c         2476 g         4836 t
ORIGIN      
        1 tttttaatag atacttttta cttttttcaa ctctaacttt atttaaaaaa ttagtaaaag
       61 tatatccact ttttttctta aacaaagaaa aaaataactt ttattaagtt gtaattcatt
      121 acatatatta cttactgtta tatcttctga gtaatttttg tgaatataat ctatagtttt
      181 ctttacataa aactaagagt tggtctatgt ttcattttat caaggcatat ttattaaaaa
      241 atcaatctct atttgttaaa ataatttatg aatgatgtta gaaactgtaa tatattggat
      301 ataatataat aaacagataa actctgtatt aagaggatat atagtttatc ttaattatag
      361 gggaagtgag gatatattat ggaaagatta atggaactca tagaaagaaa atttttaaag
      421 gatgtagaac ttttagaatt agaagacata gaaaatgtaa tagaatgcaa aatgacacag
      481 ccatataatg ctcattataa tgaatattta gtaaaagtaa aaaatggaga tgaagtagaa
      541 gaatattttg tgtatttaaa acatagataa acataaaaga ggtttatatt tatttgtatg
      601 attataaaat aaatacaagc ctcttttttt gataaatata aaattataaa atttttattt
      661 atttaatttt ttcaacaaca ttagtcaata ttgaatctaa atcatcacta atttctgtaa
      721 ctccattatg agtaatatct ataatttctt tagtcatagg aagttctcct aaaagttcta
      781 agtctaaatc atctaaaaac ttttcagtag attctccttc aaataacttg atttttttag
      841 aacaatcagg acattggata taactcatat tttcaataac tccaaggaca tttatgttca
      901 tttttttagc catattaaca gcttttgata caatcataga taccaaatct tgaggtactg
      961 aaaccataac tattccactt ataggaatag attgcataac tgttaaggca acatctccag
     1021 taccaggagg catatctatt aataaatagt ctaattcttc ccaaagtaca tcagtataaa
     1081 attgctttac aacccctcca agtaaaggtc ctctccaaac aacaggttca ttttcatcat
     1141 ctatcataag gtttatagac attactttta tgttatttga gttttctaca gggtaaatat
     1201 aagagccatc agaataagct tttacatttt ttaaacccat aagtctagga atacttggtc
     1261 cagttatatc tgagtctaat ataccaacct tatatcccat tttgtttagc ttattagcaa
     1321 gtaaagcagt tacagttgat tttccaactc cacccttccc actcataaca ccaattattt
     1381 ttccaaattt attgttaggg ttattttcaa tagaacaatt tgattgacta ttacaatttc
     1441 cctttgatgg acatgaatta caatctgcca tatctattac ctccaaactt taatatttat
     1501 tagattattt gtaacctaca tatactattt tagtacaatt gaaccataaa gtcattattt
     1561 aaaagcaact tagaagattt tctattgatt tattcataga ctatagcaaa aagaatacaa
     1621 taaaaaaatt ataacattct tgaaaaagtg attattttaa tttagttata gaacttagtc
     1681 atataaatat atagaaaata ttttaatata ttatacataa cgggttattt tataagttta
     1741 actataaatt aggtttatat tttataaaaa tttgctaaaa aaaacaatta aaaattgtaa
     1801 agaaaaaaga tataattaga actgtagtaa taaatgtttt attatagata atagagtttt
     1861 aagtatctta aataatattt ggaggaatat gaatggaatt aagaccaaga agtgcaaaat
     1921 tagaagaatt tgataaagta atagaattaa taaactatgt atttagaata tcaagaaatc
     1981 ataaaccaac tatgatggaa gaatttcctc ttttactaag taaaaataat atagaaaata
     2041 tgattataat atcagaagat gataaagtgg tttctgatgt aaactatcta attcaggatg
     2101 tatctattca aggaaacaga ttgaaggttg cagctatagg tggagtatgt actcatccag
     2161 attatgaaaa gagaggctat tcatctaaga tattggataa agtagaagaa aaaatgtttt
     2221 atgatggtgt agatattgta attatatcag gaacaagaag tctatacagt agaagaaatt
     2281 gtagtttggt aaagagtttt tataaatata caataaaacc tgaagatgta aaaatagcat
     2341 atgaaatagt tgaatttgat gaaactaatt ttgaaaaaga taatgattta gataaaatga
     2401 ttgaactata taatcaaaat agtactagat ttataagaac gagagatgag tttcaaaaac
     2461 tattgcatgc agcaactatt gcctggggac caattggtta caagaaggta tttataaagg
     2521 aaaataataa tataatagga tatttgataa taagaacaat aaaaaaagaa gattctactg
     2581 taggagaagt tgctgaaata gggttaaact ctgtaaatgt tgaaaatata ttgaaatatg
     2641 ttgcaaataa atttgaatta gaatatttaa attataaagt acatgtgaaa aatcttaaag
     2701 accagttgaa atgtaatggg acaaaatctt tggactatca acaaggaact atgaaaataa
     2761 taaattttac taagttgtgt gatagtttaa gaagttattt tagtcaatat gtaaattttg
     2821 aattactaaa atacatggaa tttaaacaag ttgaaaataa atatattatt aaatacaaag
     2881 aagaagaact tgtaatagaa aatttggata agcttaataa actgtttttt gaaaaaaatg
     2941 aggaacagta caatgaattt aaacatctaa aaaatatata tgaatttgct acaaaggcat
     3001 ttcctgtaga ttttccatgg actgcaaatt taaattatca atagttttag atgatattaa
     3061 ataaaattga ttgataccta gagttagtcc ttgttaaata agagaaatta ttggatattg
     3121 aaaaagcaat tttgtaagta attagaaagg atgagtatta tatggacata cttgatgata
     3181 tagcagaatg tatattaaat atgggtgtag ataatataga gaatttagta aaaattgcta
     3241 tagataaaaa tattgatgtg gaagatattt atgaatatgg tttaaacaaa ggtatgatag
     3301 gagctttaga taagtttgag aataaggaat attatctttc agaagttata gtatgcacag
     3361 atgcattaaa taaaggtatt aatcttttga agggaactgg aaaagtaaag aaaaaatcaa
     3421 agggagtaat tctgatgtct gtagtagaag gagatactca tgagatagga aaaaatattg
     3481 taaaagtaat ggtagaagcc actggatata aggttattga cttaggtgta aatagaaaaa
     3541 gtgaagatat aatagaagaa gctataaaaa acaatgtaga tataataggc ttatcttcaa
     3601 tgatgacaac tacaatggaa aatatgaaat cagttataga cgaacttaat atgattgaga
     3661 tagataaaag accaaaagta ataattggtg gaggacctgt aagtatggag tttgcagaag
     3721 aaataggagc agatggatac agttcaaatg ctccaaaagc agttaaatta ataaataaac
     3781 ttataggagg tgaagtttaa atgttttcag atatgacttt acttcagtat ataaatagtg
     3841 aagatagagg tttttttctg cctgatatgg gtacaaatgg attgttctta ataaataaaa
     3901 aggcatatga agtatatgat agtgtagatt tgcaattaga attagcaaag actatggaca
     3961 agtattttga atcagatttt atatactcat tctgtgatgg gattacattt tgtgaaactc
     4021 tgggtttaga gacattaaga ccagattatg attttccaag tgtattgaca catactataa
     4081 ctgacagaga taaattaaga aaattagatg ttccagaccc ttatacgagt ggacgtatgc
     4141 cactcaatat agagagctta tcagttatat caaaaagtat taaaaaacca ctatatgaat
     4201 cgatacaagg tccatttaca cttgcaggtc aattagctgg agctactcaa cttttaagat
     4261 gtataataac tgataaaaaa tttgtagaag aattattgga atttacaact gaattggtaa
     4321 gaagatatgc tgtagctgca aataaagcag gagcaaaata tatttcaata gcagagccaa
     4381 catcagtaac cctaagtaaa gatagatttg atgaatttat agtaaaaaat ttaaataaaa
     4441 tctacaatga acttgattgt tggaaaggta tgcatatatg tggagacacc agagaattat
     4501 tagataatat gctaagttgt aatatagatg cagtaagttt agaccaaatt ttagactatg
     4561 aagaaattgc tccaatgatt cctagagata tagttttgat tggtaattta gacccaataa
     4621 aacttcttgg acgttctaag ccagataaga ttagaagaga gacactaaaa ttgttaaaga
     4681 agatgagaag atatgataac tttttatgtg attttggctg taactgctta aataccactc
     4741 cagtagaaaa tttacaagca gctataaagg caggaagaat tagttataaa gaattagata
     4801 ggattgatat tgatgaatta taatagtaat aaaaataaat atcttttaaa ggatataaat
     4861 ttagatgata tttttataga taagaatatt gtgctaaagt ttttggggta tggaaataga
     4921 aaagctccag atacaattag taaaatcgtt gaaagagaaa taagaaatat aaatgatatt
     4981 ttagatatca agatatatat aagtgaagtt gatattaata aaacacctaa agaatgtaaa
     5041 aaagcatttg ctgtactata tacaataggg gataaaattg atattaagat gaatgactat
     5101 atggaaaatt gcaatatgat ggcagggcta gcattggata aaataggtat agtatattta
     5161 gattatatta atgagaaaat aaaaatgtat ttaaaaggaa agtatgatag tctcaaaata
     5221 tcgcatgaaa tatatccagg agataaagat tttgaagtag agagacaaaa ggatatttat
     5281 aactatgtaa aaaataaata taatagtata gaaatagaaa tcaatgatta tcatcaacta
     5341 tcccccataa aaagtgttgc aatgcttatt ttaatggggg atgaagaaaa tttagaatca
     5401 agatgtagca aatgtcctag aaaatgcttt taactagttt tcaagactaa gatttgtttt
     5461 gtgtataaat tgcaataatt tttttagtca ttttatgtat aaactaaaaa aattattgca
     5521 aagtaatatt ctatttaatt tgaacagact tacaaactat agtagtttct ttaattcaat
     5581 aagtgtattt atttcataag ttggtatatg attagtagaa tttatactgt tattggaatt
     5641 taaccaacaa gtatctattc ctgaatttat tccacctaag acatcagagg ttaagctatc
     5701 cccaattatt aaggcagaag atttatcatg acaattaaac tttttgagag cgtatttaaa
     5761 tatttcagga tttggttttg atattttcaa ctcttcagat acaaccattc catcaatata
     5821 tccttttatt tttgaatttt caagtctttt cttttgaact gcagaaattc catttgttat
     5881 tataacaata gtatactttt ttttaagata ttgtaatata tcgattgcac ctggaattaa
     5941 aaatgtacat tcacctaaaa aatttaagta cattttgcta agagtttcag aatcaacatc
     6001 caaagatatt ttatttgcaa aaagttcaaa tctaagtaat ttaagttcat tcaaagttat
     6061 aatatttttt tcaaggtcga gccaaagctt atcacttata tttctatatg actctatata
     6121 attctcaaaa ttaaattcta aatcaaactc agatagaagt tttttaaaag catgtgattc
     6181 acttttctta aaatcaaata aagtatcatc tgcatcaaaa aatataaatt tatatgtctt
     6241 catggttttt actcctttac aagatgactg caaaaattac tttactttca ctatttgagt
     6301 cattttgcca tttatgtttt gttcctaaag gaacttttac actatctcct ttggacaaag
     6361 taaattcatc atcagccata aaaagtttaa cttctccctc caaaacatat gcaatttcat
     6421 ctccattatg agtaatccta tcaacacaag aagaagtgtt tggagctaaa tccataattg
     6481 caaattctaa attaccacta gagtctggaa ttaaaatctc atatataact tctttgcttc
     6541 caggtaaaat aacttttttt ctattatcat gtcttactat caagtttttt ttatctactt
     6601 cagatacaaa aaatgtaaat aaaggaacgt ttagagaaga ggctatagat tttaatgaat
     6661 ttacagatgg attagcaaga cctcgttcta tttggctcaa taatgatggt gtaacatcag
     6721 ctagttttgc taaatctcga atacttaagt tttgtttttt tcgaacttca gctatttttt
     6781 cccctaaatt taaatcattc atataaatat cctctctttt taatttaaaa aatattaact
     6841 taaaataatc aagtaaaatg attatgtagt atgaaattat tataacatat agaattaggt
     6901 tttgaaaaaa ctatgttcaa tataattaaa taaattaaaa tataattgac aaagtggata
     6961 cagaatactt aaaatatatt taaataaaat taagcaaaag atagttaatt tatgatagga
     7021 ggggatatta tgcaaataac gacttttgga gctatatttg gtttattaat agcaattatt
     7081 ttaattataa aaaaatttca agctgtatat agtttaatgt taggagcatt tattggagga
     7141 ttagttggag gagcaaatat aacacaaact gtagatttta tggcaaatgg agctatgaat
     7201 atatcaccat caattcttag agctttggca tcaggtgtat tagctggaag tttaataaaa
     7261 acaggggcag tagataaaat atcagaacag attgtaaaaa tatttggtga aaagagggct
     7321 ttattttcga ttgccatttc aactatggta cttgctggag taggtgtaaa tttggatgta
     7381 tctataataa ctgtagcacc tataggatta tatataggta gaaaattaaa ttattctaaa
     7441 ttgtcaatac tattagctat gttgggaggg ggtaaagcag gtaatataat atcaccaaat
     7501 ccaaatacta tagcagtagc agataatttt tctgtcaatc tctctagtgt tatgatggca
     7561 aatataattc cagcaattat tggagttgtt attacagtta ttttagcaag tatattaata
     7621 aataaaggaa ataaagtaca atcatatgaa attttagagc aaagagaaga tttaccaagt
     7681 ttatttaaat cattatgtgg tccaattata gctatatttc tattgttttt aggaaatgta
     7741 tctcctatag ttatagaccc aataatagcc cttccaatag gaggaatcgt aactctaatt
     7801 gtcacaggaa acttgaacaa ttcaagagaa tatttagctt ttggattatc taagatgcaa
     7861 ggggtatgca tattattact tggaacaggg actatcgcag gaattataca gatgtctgaa
     7921 ttacaacaga gtacaatagg tgctttacaa tttttaaata tgccacaatt tttattggca
     7981 ccagtttcgg gtatacttat gtcattagca acagcatcat caacagctgg tgctactata
     8041 gcatcatcta cttttcatga tgctataata aatggtgggc tatcaccgat ttcaggtgca
     8101 tcaatagtaa atgctgggtc tagtgtattc gagcaattac cacatggctc actatttcat
     8161 actagtgcag gtagtataaa catggatata ggtgaaagat ttaagttaat tccatatgaa
     8221 gcattgatag gaatagtaat gactataatt tcaacatcta ttcaattagt tttataataa
     8281 atataaatat ttaatgaaaa aagtaggatt ttgttattaa atattgaatt tattatagta
     8341 aaaaagaaga ttggagtttt aaagtgaata aaaaaaagat tgttattata gggattattt
     8401 attcattttt agtagtattt tcacttacaa atatgtatgt aaatatggag tataatctaa
     8461 atgtatttga atatattaag aagtcacttc catttacaga agaagaaaaa aagtggttag
     8521 aaaaacacaa gaatttgatt tacagctctg accaaagttc acctcctttg agatataaag
     8581 gtaaggaaga tggacagtat aagggaatta ttgttgattt aataaattct ctgtcaattc
     8641 aaattggtag agatttttat tttaaaccaa ataattggtg gaaagaatct ttcgtaaatt
     8701 caatagatga tagtataaag ttttttgact tgattccatc aaaagaaagg gcaaataaat
     8761 ttatatttac tgacccaata tacactttga gtgcaaatat attgaaagat aaaaagtccc
     8821 aagatataaa tagttacatg gatttaaagg ggaaaacagt agcaattcca gaaggtgatt
     8881 attctataaa ttttttaaag caaaaaattc aagatataaa cattttattg actccagata
     8941 taaagactgg agtcaaccat ttaatgtctg gaaaagtaga cgcagttgtt ggtgatgaac
     9001 cagtattgcg atactatata aataattatg gactttcaaa taaatacagt gttttaagta
     9061 atcctatata tactaaaaaa gcagttcttg ctgtacctaa acaatatgaa gagctggtta
     9121 gtattttaaa taaaggcatt tttaaacttc agaaaaatgg agtgtataaa gatttaaaga
     9181 aaaaatggta ttctacgtat aatgaagttg atgatatatt gtatgagcga ggaattgtgc
     9241 ctagtattta tctttttata ggtataattt taatatcaat ttatgttttt tatagctata
     9301 catatttgtt aaagatagaa atcaaaagga aaacagaaca ggtaatagaa aataaaaaaa
     9361 ctttagaggc aacttttaat agtattacag atataataat gcttgtagat gaaaataaca
     9421 atatagtaga atcaaacaaa gtactatatg actttatgga tgaaatgagt tataagatag
     9481 cagatttgat tagcatgata aaaggtgtta ttgaaaatac cttctctgaa aatactaata
     9541 aaactagtga aatagaaata cacaataaaa ttttaaagat aaacactttt cctgtagagt
     9601 ataaaaaaat aatactgagt atatagtagt tcttattaaa gatataacta atgataaaat
     9661 agtagaggcg aaattactta gagaaaacaa aatgatatct ataggacaat tagcttctgg
     9721 ggtagcacat gagataagaa atcctttggg tataataaga aataattgct atctcttaaa
     9781 agataacgtt acaatggaag aagtaaatga ttgtgttaaa tcaatagaaa gtaatgtaga
     9841 tagggcaagt aatataataa ccaacttgtt aaatttcgca agaatatcag atgataattt
     9901 agaacatata aatataagaa attttattga aaatatagtg aaacttcaat ataaaatgtt
     9961 acagttaaag aatgttgaaa taaaaattga ctgtgaacat aatttaatat gctatataaa
    10021 tggagaatcg ttaaaacatg tatttataaa tttaatttct aattcaatag atgcaataca
    10081 tcaagatgga aaaattatta tatactgtta tgaaaaaaat cattgtttat ttatagattt
    10141 taaagataat ggtgaaggaa taaaagaaga tgccctaaag gacatattta atcccttcta
    10201 tacaacaaaa ccaattggtg agggaactgg tttaggttta tatattactt ataatgagat
    10261 taaaaagaat aatggagata taagtgttga aagtaaattg ggggttggaa cttgttttca
    10321 tataaaaatt ccattaaata aagaggtgac tatataatga aaatactatt agttgatgat
    10381 gagctagaat atggagtggt aatgaaaaaa attctacaaa agaagggcta tcttgtagat
    10441 atagcactaa gtggtgaaga agctataaat ataattaaaa aagataaaaa ttatgatttg
    10501 gttttatctg atgttatgat gaaaaatatg gatggagtac agctattaga tagaattaaa
    10561 tctattaata gagatataga ggttatatta gttacaggat atggaagtat tgaaaatgca
    10621 gttgatgcta tgaaaagagg agctttatct tattttataa aaagtaatcc cattgaaaat
    10681 ttattagaag aagttgaaaa ggtaaaggca agtaaaatat ctgtttctgt acaaaaaaat
    10741 aatctggaat ttactttgga atctaaaaat agagatttta ataatatcat aaaaatagca
    10801 aaaaaagctg cttgtaaaga tgttaatata ttaatcttgg gggaatctgg tgtaggaaaa
    10861 gatatattag ctagatatat acatagcata agtccaagaa agaatgagat atttgtacct
    10921 gtaaattgtt gttctttttc agaaaatcta ttggagtctg agttgtttgg tcatgaaaaa
    10981 ggttctttta caggagcagt agatagtaga aaaggtagat ttgaactttc gaataaagga
    11041 actctatttt tggatgaaat aggagatata cctttaaatg ttcaggtaaa acttttgagg
    11101 acacttgagg acaaatctat agagaggata ggaagtaata aatctattaa agttgacttt
    11161 aggttaattt gtgctatgaa caaagaacca aaagtggaaa tatcaaatgg aaatataaga
    11221 gaagattttt tttatagaat aagtacaata acaataacta taccaccact aagaaagaga
    11281 agagaagact taactactct tatagaattt ttcttaaata agtaccaaat tgaacatgat
    11341 aaaaagatac actcgattga taaggaagta aaggattttt tattaaatta caattatcca
    11401 ggaaatataa gagaattaaa aaatataata aatagacttg tagttttaag tgaagaaggc
    11461 aatttatcaa aagacaattt aaatttaata tcaaataatg tatacataga tgataaaata
    11521 agtattagac ctttaagaga aataagaaaa gagtttgaat gtgagtatat tgaaaaagtt
    11581 ttatctttgt gtggaaataa tatttcaaat acagctaaaa aattggagat tagtagaaga
    11641 cagttaacca ataaaatatc agagtataat attaagtgag aaaaaaactt ctcattaagt
    11701 gagaaaaaaa tttctcaaac tgtattaaaa gataaacaga aaaaatctgt ttatcttttt
    11761 ttattataat tttaaaaatt aatatttaca ataattattg gtaaattcaa tattggtgta
    11821 aattaaaaat gaaaatttct atgacattaa tacatagaat atatatttta aataattggc
    11881 atagaaaatg ctttatatat taataaacgt aataaatact tttaattgcg attaaatata
    11941 ttaaggggga ttttcatatg aagctagagc tagggaatat atttataaaa aatgttgaat
    12001 ttggagaaaa gacagaagta aaagatggag tactatatat tagtagcgaa gaaatagaaa
    12061 agatagtatt agaagatgaa aggattgtaa gtgtaaatgt tgaacttgca agacctggcg
    12121 aatcaataag aatagctcca gttaaagatg taatagagcc tagagttaaa attggagatg
    12181 aaagcaaaat atttcctgga actataaata aagtgaagac tgtaggaagt ggaagaaccc
    12241 atgtactttt aggagcttgt gttgtaactt gtggaaatat agttggattt caagaaggcg
    12301 taattgatat gagtggtcct actgctaaat atacaccttt ttcaaaaaca aataatatat
    12361 gtattgttat aaaagcaaag gatggtatag atactcatga ttatgaagaa gcagctagaa
    12421 ttgcaggatt aaaaataggt gcatatgtag gtgaggcagg aagagaagtt gagccagatg
    12481 aagtcattgt atatgagaca aagccattat taaaacaagt aaaagaatat ccagacctac
    12541 caacagtagc atatgttcac atgttacaat cacaaggact tttacatgac acttattact
    12601 atggggtgga tgctaaacag atagtgccta catttatgta tccaactgaa ataatggatg
    12661 gagctataat atctggaaac tgtgtagcac catgtgataa ggttacaaca ttccatcatt
    12721 taaataatcc agttatacat gacttatata aaagacatgg aaaagattta aactttatag
    12781 gagttatttt gactaatgag aacgtattct tagtcgataa agagagaagc tctgatatgg
    12841 tagctaaatt aatagaattt ttaggagttg atggagttct agtgacagaa gaaggttatg
    12901 ggaatccaga tactgaccta atgatgaatt gtagaaaatg tagtgaagtt ggagcaaatg
    12961 tagttttgat aacagatgaa tttcctggaa aagatggcaa gagtcaatct atagcagacg
    13021 ctactaagga agcagatgca gttgtttctt gtggtcaagg aaatctagtg gttcatttcc
    13081 cagcaatgga aaaaattata ggaactttag attatgttga aatgatgata ggtggatata
    13141 aaggatgctt aaatgaagac ggaagcatgg atgcagagct acaaattata atagcatcaa
    13201 ctatagcaaa tggctataat catttgacag caagatatta ttaaataagt atttgaatat
    13261 aaagttctgt tgatgtaatc attggtctaa ttgatgtaat aactataaag tgatttggag
    13321 gattaagaga tgagtaaaat cagagtggtt cattatataa acaatttctt tgctggtata
    13381 ggtggagagg aaaaagcaga tattccgcca gaaaaaaggg ctggtgtagt aggacctgga
    13441 atagcatttc agagtcaatt taatgatgat gcagagataa tctcaactgt aatttgcgga
    13501 gatacgtatt ttggtgaaaa tattgagagt gcaactcaag aacttttaaa aatgataaaa
    13561 gaagaaagcc ctgatttatt tattgcaggc ccagcattta atgcaggtag atatggagta
    13621 gcttgtggga ctatttgtaa ggcagttgaa gaagagttaa atattcctgt aattacaggc
    13681 atgtataaag aaaatccagg tgtagatatg tttaaattag atttgcatat agtgtctaca
    13741 ggaaattcag cagcaagtct tagaaaggta gttccaataa tgacgaaact aggtcttaaa
    13801 ttggttagag atgaggaaat aggacctcct gaagaggaag gatatataat gagaggaata
    13861 agaaaaaatt tcttccatga acttagaggg tctgaaagag caattgatat gttggttaaa
    13921 aagatgaaag gggaaagctt tgaaactgag tatccaatgc ctgaatttga tagagttaca
    13981 cctatgaaac ctgttaaaga tttatctaag ataaaacttg ccctagtaac ttctggaggg
    14041 atagttccaa ttgataatcc agacaaaatt gaatcttcaa gtgctactaa atatggaatt
    14101 tacgatttaa cagatatgga ttctatgtca aataaagaat ttacaactat acatggggga
    14161 tatgatagag catatgtcct tgaaaatcca aatttagttg tgccactaga tgtagttaga
    14221 gagcttgaaa aagaaggtgt aattggagaa ttagtagatt attttataac tactacagga
    14281 acaggaacga gtgttggcaa ttctaagaga tttggagaag agttttctaa aaaattgtta
    14341 gaagatgatg tagatgctgt aattttaact tctacatgag gtacttgtac tcgttgtgga
    14401 gcaacgatgg taaaagagat tgagagagct ggtatggcag ttgttcatat atgtacagta
    14461 gtaccaatat cacttacaat aggagctaat agaattgttc cagcagtagg gataccatat
    14521 ccacttggaa atcctatgtt aggagaagag gaaagtaaaa aaattagaaa gaatatagtt
    14581 ttaaaagctt taaatgcact aacagaagat atagaagaac aaacagtatt tgaataataa
    14641 aaatacaaca caacactgaa ataaaaaaag atatcaaaaa ataatttttt aaaattattt
    14701 tacgatatct ttttttatta ggcaaattcg aaagttgcat attatttgca ta
//
LOCUS       DAEMHW010000088        14714 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEMHW010000088 DAEMHW010000000
VERSION     DAEMHW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14714
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.088"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..2253)
                     /locus_tag="KPK67_002221"
     CDS             complement(<1..2253)
                     /locus_tag="KPK67_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2459185.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDEN"
     gene            complement(2612..3556)
                     /locus_tag="KPK67_002222"
     CDS             complement(2612..3556)
                     /locus_tag="KPK67_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459186.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTDGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELSDSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKLLSWIEVSDGHFVYSSKSDINKYQQNLKVV"
     gene            complement(3549..4574)
                     /locus_tag="KPK67_002223"
     CDS             complement(3549..4574)
                     /locus_tag="KPK67_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459187.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEILLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(4649..6217)
                     /locus_tag="KPK67_002224"
     CDS             complement(4649..6217)
                     /locus_tag="KPK67_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2459188.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(6330..7241)
                     /locus_tag="KPK67_002225"
     CDS             complement(6330..7241)
                     /locus_tag="KPK67_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2459189.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(7225..8211)
                     /locus_tag="KPK67_002226"
     CDS             complement(7225..8211)
                     /locus_tag="KPK67_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2459190.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            8571..8972
                     /locus_tag="KPK67_002227"
     CDS             8571..8972
                     /locus_tag="KPK67_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2459191.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            8975..9373
                     /locus_tag="KPK67_002228"
     CDS             8975..9373
                     /locus_tag="KPK67_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBG2459192.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(9823..10047)
                     /locus_tag="KPK67_002229"
     CDS             complement(9823..10047)
                     /locus_tag="KPK67_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBG2459193.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(10082..11449)
                     /locus_tag="KPK67_002230"
     CDS             complement(10082..11449)
                     /locus_tag="KPK67_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2459194.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(11533..13068)
                     /locus_tag="KPK67_002231"
     CDS             complement(11533..13068)
                     /locus_tag="KPK67_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459195.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVLDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDVILVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            complement(13091..13525)
                     /locus_tag="KPK67_002232"
     CDS             complement(13091..13525)
                     /locus_tag="KPK67_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459196.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(13785..14696)
                     /locus_tag="KPK67_002233"
     CDS             complement(13785..14696)
                     /locus_tag="KPK67_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBG2459197.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIIPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
BASE COUNT         4656 a         2593 c         1525 g         5940 t
ORIGIN      
        1 attttcatcc ttatctatat cttttttgct aatatagtca attcctctaa tattaattct
       61 ttgttcattg attactgcaa caatattact actttcttta agtttcgtaa tatttaaact
      121 taaatctttt tctgttttat atgggacaga tataatatct attccatcta agtacctact
      181 catcttttct tctaatattt ttcttgtttt attatctaca ccttctgaaa catgtgatgc
      241 tataataatt gttttagaca gattttcttt attttgaatt tcattagttc caaaatagta
      301 ctttctcgtg ttagttatag agttgtatac gtcatttaaa ggtaatccca tattggcctt
      361 ctgaactgct tctaacacag ttaatgtcgt caccatagga attactctta ctccacactt
      421 tttttctttc tttataattt catcaaaaga gtttaatgaa cccatatctg taaatagtaa
      481 tacatcttta ttgtctatat tattaattaa atttttaaaa tctacaatta cttcatcata
      541 gcttttatct aacttcatat cgaaagaaat agcactgcac tttccaataa tatcaccaac
      601 tgcttttacc atactacttg ctgtagtttc tccatgcata gcaactataa tattaatgtt
      661 attcactatt ttattttgtt catcttgttt agcttgtact atgaataatg taatatatgc
      721 tacagtttct atagataaaa ataaatcaaa ttcattttca attattccaa ctatttttag
      781 tgctgtttta aattcacttc tgttatttct tctaatttga ttcaaatcta tgttgtttgt
      841 agagctactt ttatccaata cgctttttat ataaattgcc attgacttaa atattgattc
      901 acttatatct atttcttcat cttctttaat aatttgtctt attgattcac acatgctttt
      961 aaacttaaca ttattaaata gagaagcatc tgctatattc atttctacag atttattaag
     1021 attagtattt actgcattaa taatatttat atcattgtag ttaataactt cttcttctcc
     1081 taatgacgta aatacaaaat attctattcc atgtgttata acttcatctg ctttaataga
     1141 cttctctcta taatcatcac tcacttcatt tatagattta tcaaaatatt ttttctctat
     1201 ataaactggt tctttattat ctcttttata tcttaggtat ccatttgcaa cagctaattt
     1261 tatatcattt tgaagttgac ctacattatt tttacaatca taaagtaata gtgatttaat
     1321 tatttcttta tgtactttta ttgaaataga aatacttttt gattcttcct tgaaaaaatt
     1381 tttgactaat tccattcttt caatcattgt cctatctctc agtgatggta tttctatttt
     1441 cattggtatt cttctattga atgtatctaa tagtgttgat gtcggatttt ctgtagtagc
     1501 acatattatt aataccttag actctctttc tttcgttgca tctcctagtc ttttaaatgt
     1561 tcccttatct ataaaagtaa acaacatttc ttgaccttct gctggtagtc tatgaatttc
     1621 atctaaaaat agtatccctc catttgcaat ttccaaaaga ccttttctgg aagattctgc
     1681 acctgtataa gctccttgtt ctaccccaaa aatatgtccc ataagtaatt gtgcattatt
     1741 agcataatca gcacaattaa atgttacaaa aggagcttta ttttttctaa ttccactatc
     1801 tatagaataa tcatacatat atctagcaat catactcttt cctactcctg tttctccata
     1861 gataatagta tgaagtccat ttgtaggata gagcattgcg accttgcatt tttctattat
     1921 aggtcttaaa ctatgttctt tacctattag tgtttcaaaa ctatcttttt ttattttgtt
     1981 ttctactttt gttccttttt ttatgccaag caactcactt tgtgtatcaa gcaattcatt
     2041 cttgttaagg ttaagatttg cagggatata atacacaggt cttcctttta tcttcgtaac
     2101 cataccctca ttgaataatt ggtttaaata tgtacttata ttgtttcttg caattcctgt
     2161 cttttctgat aattggttag ctgtaatccc tttttcattt tccctatatg atgattctaa
     2221 aactttcatt actttatctt ttttatatat cattacaagc tcccctacat tataatattt
     2281 tattaactaa aacccctata cacactttat tttttaataa actcttattc tttaactaag
     2341 gttataattt atcaaacttt ccttacttta aattatagaa aattattgta gaaaaaataa
     2401 gattattgct cattttttct acattactat ttttaatttt atagtacttt cttaacaaca
     2461 tactttactt gtttttaatt ttattatatt tgaatttatt atatttgaaa agtagaaata
     2521 aaaaacccta ttttggagta gttttaaaat ttctccaaaa atagggtttt agtttgtatt
     2581 tcaaataaat acctgttcaa gtatttttat actaaacaac ttttaaattt tgttggtatt
     2641 tatttatgtc tgatttagaa gaatatacaa aatgtccatc tgaaacctca atccaactta
     2701 ataacttttc atttgaacaa tcatatccat tagaattata ctctattcta tttcttgact
     2761 tagcatatct agggtcagga acaggaattg cagaaagtag tgattttgta tatggatgaa
     2821 taggattatt atacaattca tttgaatctg acaattctac taattttcca ttatacataa
     2881 ctcctatacg gtcactaata tgtctcacca tagataaatc atgagctata aataaacaag
     2941 ttaaattctt ttcttgttgc aattttttaa ataaatttac tatttgtgct tgtatagaca
     3001 catccaaaga agctataggc tcatcagcta ttataaattc tgggtctact gaaagagctc
     3061 gtgctatgcc aatacgctgt ctttgtccac ctgaaaaatc agatggatac ttatttgcat
     3121 aacttctatc aagtcctact agctttagca tctcatatac tttattcatt ctatctgttt
     3181 tatttttaca gataccttga atttttagtg gttctgaaat tatatctgca attgtcatac
     3241 gtggatttaa tgatgaagta gaatcttgaa aaataatttg catacttttg tttacatctt
     3301 tttttataaa tttgtacgtt tttttatctg atatgcaatt tcctttataa attacttctc
     3361 catcagtaga ttcatgtagt cttattatag ttttaccagt tgtagatttt cctgaacccg
     3421 actctcctac caatccaaat atctcaccct tatatatatc aaaacttata tcatcaactg
     3481 cttttacagt agtacttttg tccaaatgaa aatattgctt taagtttttt atttccagta
     3541 ttttttctct attcattaga aatcaccctt ccacaattta ctctaactgg tacatctact
     3601 tcaggtgcat ctgggtgcaa taaccaagtt gctgcactat gtgtatcatt aattttaaac
     3661 attggtggct ctttttcata atctatctta agtgcatttt tattccttat tgcaaaagca
     3721 tctccttttg gtgggttcaa taaatttgga ggcattccag gtatattata taaatagtca
     3781 tcctgcgaat ctaaagtagg taatgaaccc aacaaccccc aagtatatgg atgtctaggg
     3841 tcataaaata tatcctctac agttcctatt tctacaatct ttcctgcata cataactgct
     3901 attctatcag ctatagttgc aactactcct aaatcatgag ttataaatat tattgacaaa
     3961 cctattttat gttgtaaatc ctttattaaa tcaattattt gagcttgtat agttacatct
     4021 aaagcagttg taggttcatc agcaatcaat acatctggat tacaggcaag tgctatagct
     4081 attactatac gttgtctcat tccacctgaa aaatgatgtg gaaattgttt aaatcttttc
     4141 tcaggattat caattccaac caattcaata agctctatgg ctctcttttt tgcttcactt
     4201 ttacttatac cttgatgaat gattacagct tctgcaattt gctttcctat tgatatagtt
     4261 ggattgagag atgtcatagg gtcttgaaat atcattgata tattctttcc tcttatatct
     4321 tccatctctt tttcagactt cttaaccaaa tcacttgatt taagtaaaat ctctccattt
     4381 ttaatatatc cattgtatgg gagtatcctc atcaaactct tacacagtac agatttccca
     4441 caaccagatt ctccaactat agccaatgtc tcaccttttt ttagattaaa agatacattt
     4501 cttacagcct gaacttctcc ttcttctact ttaaaactta cagatagatt attaacctcc
     4561 aataaatgtt ccaaaaacca tcaaccccta tgctattcaa accttaggga tatattttta
     4621 tccctaaggt ttctaattaa tttcagtatt attcaattgt ccagtcagct atgttccaga
     4681 atatacctac accatgatga cctaaaactg tatcaggtgt taatcctttt atgtttggtt
     4741 ttccaacata tattgcatct atataagcaa taaatgtata aggcatatcc ttagtcattt
     4801 ctacttggaa ttgtttatat aactttaatt tttcatcttt atcttctgtt tctcttgctt
     4861 tttgaagtat tttatctata gtaggattag agtaagcact gtaatttgca cctttatctg
     4921 ttccaaatac tttatatgta tggtcatctg ggtcaaatgg acttccccaa cctattaaat
     4981 gagcatcttg attagcccaa tctgtttcag ttacaacaac agctttagca tcaacaccaa
     5041 tttcttttaa ctgttgtgca catattttag ccatatcaac tctaacttgg tcactctcac
     5101 ctgctgttat ctcgaaagct aacttagtac cttctttttc atagattcca tcagaaccta
     5161 atttccaacc tagcttttca atttcttgtt tagctttttc tggattatat tcaaactttt
     5221 ctatatcagg attattatat ggtcccattt gtaaaggtga atatgctggt acaccatgtc
     5281 ctaataatac actgtcaact attgcttttc tatctattgc atagcttaaa gcatttggta
     5341 atcctttagc ttttttatct ttaaagaatt tagaattaaa attgtataat atacctctat
     5401 agtcggctgt tttcattata tttactttaa aattcttttc gtctttttca aagttagaca
     5461 tatctttagg agttatttga gctaggtcta attctcctga ttttaattgc attgccttag
     5521 ccttatcatc tggaacaatc ttaaagacta ctttatctaa tcctggttct tttacaaaat
     5581 aatcactatt cttaacaagt gttatacttt gacctttgtc ccatttttca agtttaaatg
     5641 gtccagtacc tattggtttt tgattaaatt catcagtagc tatatcctta ccttccaaag
     5701 catgttttgg taatactcct actgttaaat aatcaagcat tgctgtattt ggagctttta
     5761 aagtaatttt tatagtatta tcattaacca catcaatttt tgttatatct tcataatttg
     5821 atgctatctc agaagcatta tctggattca ttatagtttc tattgtaaac ttaacatcat
     5881 ttgctgtaaa tttctctcca tcatgccact taacatcatc tcttaaatta aaagtatatg
     5941 tatttgttgc ctcatcaaat ttccagtcct ttgctagaca aggaactacc ttattatttt
     6001 catcatgagc tgttaatcca ttaaatatta acgaatttat ttctccatgt tcgtatagtg
     6061 caggatttat acttgtatag tcattacttc cataaacaag tacctttcca tctttaggcg
     6121 tatctgcctt cttatctttg tctcctccac tagaacaacc tgccattaga ctaagtatca
     6181 ttactaaact taaaactttt aacttcttta atttcatgat gctccccctt ttgcttttta
     6241 taaaatattt ttcaaagctt tgaccccatt tatttaaata gatatttcaa atacctcagt
     6301 atttgcttat ttagtactca aatgctgatt tataaattac tagatttttt attattattc
     6361 tttcttatat aattacctat atttgttata cacaccaatg ttgtaaccaa aaatataccc
     6421 ggaatcagta taatccacca tctatttgaa agtaatgctt cctctgacaa agataacata
     6481 cttccccaag aaacaatttc tattggtaaa ccaattccta aaaagctaag tgtcgattca
     6541 gtaccaatag ctgcgcctat gtttgttaca accatgaaca ttattgatgc cacaaaattt
     6601 ggaagtaaat gttgctttaa tacatagaag aatcctcctc ccatgctctt agctgctagt
     6661 atgtattcgc tatttcttat ttgtctaact tctgttctca ctatcttaga tatattcatc
     6721 caacttgtta tccctattac tatggacata gatactggat ttgaatttcc aagtatagct
     6781 tgtacaaata ttataattaa tattgaaggt atactaagta tgatttctgt aaacctcatc
     6841 attgcatcat ctacgagttc agatgctgaa ccacttatac taccataaac aattcctata
     6901 acagttgata ttacagtgga aaatagtcct ataaataaag atatttttcc tccataccat
     6961 atcatagaat atatatctct acccattgag tctgttccaa ataaaaaagt tttatttgga
     7021 gctaaattag aacttgctaa atccatgtat gttggttcat gtgtcattat aaaagatgag
     7081 aatacacttc caatgactat gattgataaa ataataattg atatatatgg taattcttta
     7141 atttttttag ataaggtttt ctttgtttct atatgttcat cttctgctac taaaatataa
     7201 ttttctccta caatttcaaa atcactatat tgtggcttca ttagtatcac ctctatcata
     7261 tttcattcta gggtctattt tattgtttat aatttgagct aacatattcc caaatactac
     7321 taaagcccct gttattaagc acaaaactaa taacatatta taatcatgat actttgcact
     7381 ttcaaaacaa agtgttccta acccaggata agaaaaaact ttttcaacta cataagttcc
     7441 tcctagtata tgaggtatag aaatggccat tatactaata tatgatggca ttatatttct
     7501 caggcaatgc ttaaaaacaa tagttctatt atttaaacct tttgctttac ataaaagcac
     7561 ataatcttct cttatttcct ctagtaattt atttcttatc atatatgtgt aataccatag
     7621 atggcttaat ataagtactg taagaggtaa gattagatgt gatatcctac ttaatatact
     7681 ggactcttct cccattgaat aagcaccact acttggaagt atacttaagt ttatactaaa
     7741 tataagtatg agtactaagg caacccaaaa tgatggaata cagttggtta ttgttcctaa
     7801 tttgcatatg attctatcaa tgagcttatc ttcatgtaat gtacaaaata ttcctaacaa
     7861 taaggctagc acaaaggtaa gtatatatcc tgaaccacct aatattatag tgtttatata
     7921 gacatcattt ataacagaag taacattttg tttatattta aatgaaattc caaattcccc
     7981 ttttgatgca tttgaaatcc attttatata ttgactgtat attggttcat ccaaaccaag
     8041 ttttttcata gcattttctt tttcttgtgt actcattctt tctactccat ccccatagta
     8101 agagattaat gggtctcctg gagcaagtct tgccatgtaa aaaacaataa aagataataa
     8161 aaacattact aaaataaact gcaacaactt tttcagtata tcctttaaca aaccccaacc
     8221 cccctttgtt gcaataaaaa aaaccacata aaagcaaacg taaagtaacg ttaaaacctt
     8281 cgtggttcca ttatcattaa acaaaattaa attttctata tttatctatg agttaatttt
     8341 agcatcattt attaactgtg taaacaaact ttttaaaaat tgttaaaatt tcatcaaatt
     8401 attttattta tacctaaaat aaaattttta tattctattc ttcattattt tctataaaat
     8461 tataaaattt taggttgact atataagaaa agtatattat attagtttgt atgcaaacca
     8521 tttaagaggt gattttattg aaaagtaatt atgactgttt tagaatagcc atgctcttaa
     8581 aagaacttta ttctaaaact atgtacacag tagaagaaaa ttttaaagaa aatggattaa
     8641 cacaccaaca gattatagtt ataaaactga tagctcataa tcaagaactt acaatatcac
     8701 aactatgtga tgaaatgtct ttagctaaag gtacggtttc aggcataatc agcagattag
     8761 aacagattgg atatatagaa aaattcaaga aatctaatga taaaagaaat acatacgtta
     8821 agtttacaac aacaggattt gaatttgcca caaattttaa aattaaaatg caagaaagtt
     8881 ttgatgatat ttttaaaaat tgtgatgaaa acgaattgag tgacttagta aaaaatctta
     8941 gaaatatatt ggcaaaagta aaggagagat aattatgaat tataaattaa tacttgcaat
     9001 agtatttata acatcagctt taattttcta cactataggt gtatttggag aaagaaaggc
     9061 aaaaatttta aaaaagaaac atgtaattat attttggctt ggttttatat ttgatacttt
     9121 aggaacattt acaatgagta acatagccaa cagccatact tttgaagtaa aatctgcttt
     9181 atcacaaaac cttcatagta ttacaggtct tcttgcaatt gtgcttatgc tttttcatgc
     9241 aagttgggct acttttgtcc tatacaaaga tgatgaggaa aagaaaaaat tctttcataa
     9301 gtttagtata gttgtatgga ctatatggtt agttccatat tttataggta tgtttattgg
     9361 tatggctgga tagtaaaata aaaagtgtgt aagaaatact gtaggtaaat ttgtataaga
     9421 caaatttatc tacatccttc tttcacactt ttacatattt atttataata ctccattatt
     9481 tctttcatgt ttcttaatac ctatatagaa tatttaattg tttctatagt attttattat
     9541 tttctcacac tttttcatat atatattgtt ttactatttc ttcatatgta tatatgaaat
     9601 attccattat ttctataaat gttttattat ctttctcata ctttttcata tatatattgc
     9661 ttcactattt tttcttattt cttcatatac atatgaaata tttcattatt tctataatgt
     9721 tttattatct ttctcatact ttttcatata tattgcttca ctattttttt tacttcttta
     9781 ttaaatactt atgtataaca tacatttaat aaacgctaac tattaaacac ctaatacttg
     9841 agttactcct ggaacttcac tcaccaaaac attttcaata atagctttga ttgtcatagt
     9901 cgctcctgga cacccactac atgccccttg tagtctaact aaaaccacac cattttcatt
     9961 tacatctata agctctacat ctccaccatc tctttgtaat actggtttta tcttttcctc
    10021 aagcactttt tcaacttttt ctctcatagc tattcccccc agaaatattt tattaccaaa
    10081 attataccaa tggtttaaat ttttgtccat ctaatgattt atcaaattca atttttgctt
    10141 catcaataat tttagtatcc ataaatacat cataagcact acaagccagt actttagctg
    10201 aatttatcat agcagaaaat cctatatttg aacctgctga tgaacatgac tgccatgtat
    10261 gacttgcaat tccaacaggc caagctgcca ttgcaaattg agccactggt attacataac
    10321 ttacatctcc aacatcagta gaaccagcta gtcctttatc tttgtcaccg tcaactattt
    10381 ccttgtgtat atagctttct acaatagatt tatcattttg taatgatgtt gctacaccta
    10441 atctttcttc tctagtaaga gtatcacaca acttttttgc aaattcttct tcttcaggag
    10501 tatttttagg gcaaccaaca aaaaccatat tgtcataaac taaatcagtc aaacattggt
    10561 ttggtatata gtcatatatt ccatctgtaa ccttatactc catttcagtt tctgtcatca
    10621 ttgctgctcc ttgagctact tttattaatc tatcaagaac gtgttctgca tcctttgctt
    10681 ttttacctct aataaagtac caactttcag caaatccagg aactacatta ggtcttccac
    10741 caccattagt tataacatag tgcattctta tagagtcaat aacatgttct ctcaaataat
    10801 tagctccaac attcataatt tcaacagcat caagtgcgct tctaccatta tgaggagctt
    10861 gagctgcatg ggctgttatc cctttaaatt taaattttac agataagttt gccagacttc
    10921 cacctctcca aggtgcattt atatcaaaag gatgccatgt aaatgcacaa tctacatcat
    10981 caaaacagcc atttatacac ataactgtct ttcctccacc ttcttcttca gcaggacaac
    11041 caaaatattt tattgttcca ttaaatttat tttcttctat taattttttt atagaaacaa
    11101 ctgctcctac acctgctaca cccaacaggt tatgcccaca tccatgtcca gcttttcctt
    11161 ctaccaatgg ctttctttct gcactcacac attgtgatag ctctggtaaa gcatcatatt
    11221 ctccaagtat gccaatcaca ggtttaccct ctccaaaact tgctataaat ccagtaggta
    11281 aattttctac ttcttcaatt ttaaatcctt ttgactttag gtattctttt tgaatgctac
    11341 ttgcatactt ttcttcaaag gctaattctg gattttccca tattttctta gataaatcaa
    11401 ttaattcttc tcttatttct tcaacattat ttatgatata ctctttcata attttcctcc
    11461 aaatatatgt atttttgata tgctttacag attatattat atactaacat tctatttatt
    11521 cacaattttt tgttaattaa ttttattcat atgctcttct atggagtata tcttatcttc
    11581 actttgcctc ttcataaatt taatgtactc tatcttccat aaagtagttt cagttttctc
    11641 atttaacata aattctaata tattaatttc tctcattcta atatcttcat aaaatactac
    11701 actacccaat attgccaatt ctaattcact taatgatgca aacaacatta aatactcttc
    11761 tccaaataaa cattcagatt caagaatatc gataaagtct tttttagcat tgatattttc
    11821 ctttattatt cttatttttt caattataga tgtactatct aatatctcat tgaatatctc
    11881 tctaaatgat tcatcatcca atttactttc atcatcaaca atataatctt ctaagtgttc
    11941 atttttattt ttactcacag ttaccatact tttaaaagtg ttacttttta ttgagcttag
    12001 cacagattca ataaatatct tttcatattt tttaatatat cttattacat cttcatcttc
    12061 tatgcttaat ctttttatta atacttcaat tgcttccata agtagtaaag ttattttttt
    12121 atttattcta atttctctgt taatttcttc aaaatcatta taatccaaat tactatctac
    12181 ttcagtgtta aaactatcat ctacttcaat caattcaatt atattacttt cttcagagcc
    12241 tataacttta tcttcagtaa tattattgta catattttta aattgttttt ctaaaaattc
    12301 atactcatat tttgaaattt ctaaattagt cacttcattt cctaaaatag tagaaaaaat
    12361 acaattattt ataataagtt caaataaatt tacaagtata acatcacaat ctattttata
    12421 agtcacccca tagtcataaa ataatttttc tctatctttt ttcttaaata ggttacaaaa
    12481 tctattttct atatctatac tctcaatata attcttcaca taataaatac ccttaacatc
    12541 catatcatct agtgccaatg gataatctat acttactgga acgtcctgag cttcaaattt
    12601 tacaatataa cttttaaaga aacttgataa ctccatcaag gtatcattat atgctattga
    12661 attagtatcc aattttctac tcatcattag tttataaaaa tcttttagcc taactaactc
    12721 ttcctctaat atttcaatgc ctccaaggta cactttttta acattcttag cagtcaactc
    12781 cacaactctt ttttctatat ctccaagctt atttatatat gcatcaatag cataccatat
    12841 tgaaatcaac agtttttcgg caacttctgt ttttactgat gtgctttgac catttgtata
    12901 ttttttaatc aaatccataa gtattagaga tatttcttgt tgtatattat atacttctct
    12961 ttcatctagc actttacaat ttagacattc cttaagtaga gatatggaat attggttctt
    13021 tattaaatta ttttcgttta tattaatatt acttaaatta tttaacatac aaaatctctc
    13081 ctatcatata ttaaacttct ccaaatttaa atttctttgc aaattcgtct gcttctcctc
    13141 ttacattgtc catattgcct gaaaattttt cataaaattc acccagtatt tctataatct
    13201 catcatcaca tatcttgtca tcagtttcat tttttaaata ataaaaaatt tcttgcatat
    13261 cattaatagc ctctaaataa ttgtccacat ttgtatattg tgatgtatat atattttcta
    13321 ttaactgttt tgtaactttt atatctaatt ctattcttcc ataccccttt aaagtattat
    13381 ctctcgaatt tattatatct tttacatcag attcagaaag agctagacca taatctccag
    13441 attctatatt agtttcaagc agttcatttc tttctatttc ctttaaattc tgatttatag
    13501 aactcaaaat aataggtgat atcattttta aattctcctt ttttattttt tccattgact
    13561 ttttttgttt tttatgctat atttaaatta gtatatatta ttttaccaac cacttataat
    13621 agtagtgtta aaagtggttt ttgtcaactc aaaattacta tctcaattaa acttttcaat
    13681 tttctcaaca aaaaaatacc atcatttaga tatataattt tctataacaa cttttaattc
    13741 agtagttatt tactaaaatc taaacaatga tatatttatt aattttattt atcttttttg
    13801 gctctacttc tacttttctt tacatttgag caatttctac aagaaggtga acagtattga
    13861 gatttagaat ttgtaggaat ataaatttta ccacagtact tgcatttatt taaagaagaa
    13921 ggttcacttg taattgcaaa ggcatagata gtctcaatag ctgttttcaa agaatcaaac
    13981 tcccatgcaa taattggttt atctaattgg cttatagaaa atcctatctt ttgaggatta
    14041 aaccctccag acataatagt aacactttca gttaatagat tagatgtatt tttatataca
    14101 caaatttgat taaaatgctt acacatatct ttagcaaaac ttacaatcca atctagttgt
    14161 tcagtgtaga actttgaaaa tactaaatct aaaatcaatg gtctctttcc aaaaaacttt
    14221 ggagattctt ctctctttga tacatctata ccccttctat actttttaaa atacacatct
    14281 tcatcacttg caaatggtat aaacatgttt acatattcat ctgtactcaa tatattctct
    14341 tttgtaatat aattcttttc aatcatcagt actctttctt ctccaactat atctctatta
    14401 tatacacttg aatatattaa acccaataat ccatactttt ttacaaattc aagtacttct
    14461 ttctttagtt tcatttcttt tgtctctttt gatatatctt gtaatgcctc ttgtcctatg
    14521 cgaattatat ctactattat atcatctgct acatcaaatg ggttatacat ggtaaacaca
    14581 gcatcttctg ttggaattat atataattca tcttcttcag taattttata ttcataatca
    14641 ctatacctaa tccaatcact tgcaaaattc cctattaatt ctattttttt attcataata
    14701 atcattcaca cctt
//
LOCUS       DAEMHW010000089        14631 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEMHW010000089 DAEMHW010000000
VERSION     DAEMHW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14631
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.089"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            237..1085
                     /locus_tag="KPK67_002234"
     CDS             237..1085
                     /locus_tag="KPK67_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBG2459198.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGTEEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            1164..3086
                     /locus_tag="KPK67_002235"
     CDS             1164..3086
                     /locus_tag="KPK67_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2459199.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            3168..4031
                     /gene="aroE"
                     /locus_tag="KPK67_002236"
     CDS             3168..4031
                     /gene="aroE"
                     /locus_tag="KPK67_002236"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG2459200.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4231..5448
                     /locus_tag="KPK67_002237"
     CDS             4231..5448
                     /locus_tag="KPK67_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2459201.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5684..7549
                     /locus_tag="KPK67_002238"
     CDS             5684..7549
                     /locus_tag="KPK67_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBG2459202.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELMNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINDILLSEKWKNIYFEASRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSF
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7727..9091)
                     /locus_tag="KPK67_002239"
     CDS             complement(7727..9091)
                     /locus_tag="KPK67_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2459203.1"
                     /translation="MSQLLIKNAYLITMNADREVYETGSILIENNIIKNVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYDD
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVKTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHNPAAAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9269..10612)
                     /locus_tag="KPK67_002240"
     CDS             complement(9269..10612)
                     /locus_tag="KPK67_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG2459204.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10685..10786)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            11115..11756
                     /locus_tag="KPK67_002241"
     CDS             11115..11756
                     /locus_tag="KPK67_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG2459205.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDIFNNEKIFNKIIENEVISDL
                     DSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNSFFDGGSIYRVEFKNSKLI
                     GVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVFEYTN
                     LKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            11897..13168
                     /gene="brnQ"
                     /locus_tag="KPK67_002242"
     CDS             11897..13168
                     /gene="brnQ"
                     /locus_tag="KPK67_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG2459206.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            13370..14557
                     /locus_tag="KPK67_002243"
     CDS             13370..14557
                     /locus_tag="KPK67_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBG2459207.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSLAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
BASE COUNT         5427 a         1775 c         2529 g         4900 t
ORIGIN      
        1 atacaagaat tatcttataa ataaaatcta taaacaaaat ctatgaatta ataggaaatt
       61 atcaatttgt tattagtaaa gtctcgtgat acaataaatt tataaaatct agataaatag
      121 aaatcaaaaa atacttttat ttttaaggta aaataatact actagtagga taaataaaaa
      181 tataaaatca aaaatactat taattataaa gaaaataaag ggggcaaatt taagctatga
      241 ataatcaaaa aaatcttaat attccaatta caattagttc ttatacattg ggaacagaag
      301 tttcattccg tgatagagtt cgtattgcaa aagaagctgg gtttgatggt attggattgc
      361 gtgctgaaaa ttatattgat gcaaaaaatg caggtgttac agatgaagaa atgattgcaa
      421 ttcttaatga atatgatatg aaagttacag aagtagaata tataactcaa tggggaacag
      481 aagaagatag aacggaagct caaagagaaa aagagcaaaa tgtttatcat atggctcact
      541 tatttaatgt taaacatata aattgtggat tattagagat gataccagaa gaacaaatta
      601 taactgcatt gggagaatta tgtgacagag caggtgaact tattataggt cttgagttta
      661 tgccatatag tggtgtacca gatttggcta ctgcatggcg tgttgtgaaa ggatgtaatc
      721 gtgaaaatgc aatgcttata ttagatactt ggcactgggc tagagcaaag cagacagcag
      781 aggaccttgc acctgttcca gcagataaga tagtatcaat tcaagtgtgt gatgttttag
      841 aaactcctta tgaaaaactt agggatgaat cactacatga ccgtttggct ccaggtgaag
      901 gatatggaaa cacagaggaa tttgtgaaaa taataaaaga acacaatata tcaccaagag
      961 tcataggggc agaagtaata tctgattctc taatatctaa agggttatcc actgctgctg
     1021 aaactgtatt taatgcaact aaaaaagttt tggataaagc atggtctgaa gtatctccaa
     1081 aataaactta tataatataa aaatatacag agtattataa tataaaactt ttaaatataa
     1141 aacttgaaag gaaggaaaac cttatgaaga ttagttctat gtttacacct ataagaattg
     1201 gttcaatgac agttccaaat cgttttgtag ttccccctat gggaaacaat tttgcaaaca
     1261 cagatggtac tttaagtgaa acttctaagg cttattatct tgagcgtgct ttaggtggtt
     1321 ttggattaat aactatagaa tctagtgttg tagacaaaaa agcaaaagga ggtcctcgta
     1381 agccttgttt atatgatgat agcacaatag atagttttaa aaatgtaata gatgcttgtc
     1441 atgatgcagg ttcgaaggta tctatacaat tacaacatgc tggttctgaa ggaaatgaaa
     1501 aagtagcagg acatccatta aaagctgctt ctgcaatacc tgcatcaaat ggtcgtaata
     1561 caccactagc aataacaaca gaagaaatat atgaacttat agaatcttat ggagatgctg
     1621 cattacgtgc acaaaaagct ggtgcagatg ctgttgaagt tcactgtgct catggatatc
     1681 tggttagtag ttttatatca caaagaacaa ataagcgtgt agatgagttt ggtggatgtt
     1741 ttgaaaatag aatgagatta cctcgtctta tcattgaaaa tatacgtaaa aaagtaggaa
     1801 attctctagc aatattatgt cgtataaaca gcactgatga tgttccagga ggaattgatg
     1861 tacacgatag ttcagtaata gcagcatatc tagaagattg tggaatagat ggacttcatg
     1921 tttcacgtag tatacatata catgatgaat atatgtgggc tcctacgact ttacatgctg
     1981 gattcagtgc agaattagtt actgaaataa aaaaagctgt tagtatacca gtaataacag
     2041 ttggacgtta tacagagcca cagtttgctg agttaatggt cagacaagga agatgtgatt
     2101 tagtagcatt cggtagacaa agcttggctg acccagaaat gcctaacaaa gctaaaaatg
     2161 gaaaactaga cgaaatgata ccatgtattg catgcttaca aggatgtgta ccaaatatgt
     2221 tccaaggcaa acctatagcc tgtcttgcaa atcctatatt aggacatgaa gcagagctta
     2281 agccagctga aattagcaaa gaagttcttg ttgttggtgg aggtgttgga ggtatgcttg
     2341 cagcatgggt atgtgctaaa agaggacata atgtaacttt agttgaaaaa tctgaagttc
     2401 ttggtgggca aatgcgttta gctgcatatc ctccaggaaa aggagatatt actaacttag
     2461 ttagaagtta tatttcaaaa tgtaatcaat atggtgtaaa aatatgtact aatacagaag
     2521 ctacagtaga acttataaaa gagaaatcac ctgatgtagt tattatagct actggagcaa
     2581 ctccacttgt acttcctata ccaggaatca atgattcagg attaattcat gctgtagacc
     2641 ttcttgatgg taaaaaatct tgtgggaaaa aagtacttgt tgttggaggt ggaatggttg
     2701 gatgtgaagt ggcagccttc cttggagaac aagaacatga ggttactgtt attgagctta
     2761 gagaagaagt tggtgcagat gtaatttcag aacaccgtaa attccttatg aatgattttg
     2821 aaaattataa tatacatacc attactggtg caaaagtttc taaattttat gatgatggtg
     2881 tcgactattt acttacagat ggcacagagc atgatttaaa aggttttgat tcagtagtac
     2941 ttgctatggg ttctcgtaat tacgacccat taagtgaagt tataaaagaa gttgtaaaag
     3001 aaacttatat tgttggtgat gcagtaaaag ctcgtagggc attagatgca acaaaagaag
     3061 cttttgaagt agcattaaat atataaataa taaataaatt tttctttgta aataactgaa
     3121 taggttattt gcaaagaaaa tgataatatt gggaggagat tatagttatg gaaatttcag
     3181 gtagaacagg attatttgca ttaataggta caccagttgg tcactctaaa tcaccagtga
     3241 tgtataatta tagttttaag aaattagatt tagattatag atatttagcc tttgatatca
     3301 cagtagataa ggtaaaagag gcacttttag caataaaaac ttttaatata aaaggagcca
     3361 atgttactat gccatgtaaa tctgctgtta cagaatatat ggatgaactt tctccagcag
     3421 ctcgtataat tggagcatgt aatacaattg taaatgacaa tggaaagtta gttggtcata
     3481 taacagatgg agtaggctat gttcgtaatt taaaagaaaa tggggtagaa gttaaaggta
     3541 aaaagattac aattatgggt gctggtggtg cagcaactgc tatacaagta caatgtgctt
     3601 tagatggagc aagagaaata tctatcttta atcctaagga tgatttttat aaaagagcag
     3661 agcaaacagt tgagaatata aaaaaagatg taccagaatg tgttgttaat ctatatgatt
     3721 tagaagatac aaacaaactt tatgaggaga tacaatcaag tgatatattg acaaatgcaa
     3781 ctcttattgg tatgaagccc tatgacaatg aaacaaatat aaaagatact agtgttttaa
     3841 gaaaagactt agtagtaaca gatgtcgttt ataatcctaa gaaaacaaaa atgattgaag
     3901 atgctgaagc taatggatgt aaggctattg gtggattagg aatgttatta taccaaggag
     3961 cagaagcttt taatctatat actggattag aaatgcctgt agaagaagtt aatgaattat
     4021 gctttaaata gataagagta atatcttaaa caatgaaaaa acatacgtat ttcaatattt
     4081 ttaataataa attacaaatt gtcatagtgg gttttgcctc atagtccagt taaatttgaa
     4141 gctttaactg actatacttt aaatccatta tggggatgag tttattgtac acaaaaatgt
     4201 taataaatta acaagaatgg gagaaaaatt atgaacaata aaaaatatta tcctactgcc
     4261 atagcgttgt atttttcata ttttttactt ggtattggaa tttcaatttt aggacaatac
     4321 aaaccagaat tttcatcaat gtggggggct aaaacattat cagatggaac attggatgta
     4381 agtattgttt tagctgttat tgctgcacta ggattaggga gacttatatc ttatccattt
     4441 gcaggaccta tttcagataa gtatggaaga aaagtaagtg gtcttatagg aaactttcta
     4501 catgcaattt tctttgttgg aattgtattt tcaccaaatt tttatatagc atatgtattt
     4561 gcaataattg gaggggcagc aaattcattc ttagatacct gtgtaacccc ttcttgtatg
     4621 gagatatttg ctagtttagg aacaatagca aatatgttta ctaaatttac aatagcttta
     4681 gcacaatttt tgcttccttt tataattgga tttgttgcag caaattcaat atcatttaaa
     4741 gttattttta tagtaacagc gattcttatt gtagtagatg ccattttgat agctgtgtta
     4801 ccatttccac cagctaataa tgttattgat aacaaaggca aaacagttaa atcagagaag
     4861 atgaaattta caccaacaag tatagctctt gtttgtattg gatttacatg tacatctaca
     4921 tttgtattat ggttaaattg taatcaggaa ttgggtaaat tgtatgggat ggctgaccct
     4981 acaaaaattc aatcctttta ctcaatggga gtaatatgtg cagtattaat aacttcttta
     5041 cttattaaaa aatatattaa accaataaga atattagtta tatatcctat aatagcttta
     5101 cttatgctat tagttgtata ttttgttcaa acaccaacta tttgtatgtt agggggattt
     5161 gtaataggtt attcagcagc aggaggtgtt cttcagttaa caacgtcaac tgcaaatgaa
     5221 atgtttccaa ctaataaagg aaaaataaca tctatagtta tgattgcatc tagtatagca
     5281 aactatgtta ttttaaatat agcaggtatt attacaaaat cagggggagt taatggacca
     5341 aaatatgttg tattgtttaa tgtggcaata acatttgttg gaattttact tgcattgttt
     5401 gtaaacatga gatatgaaaa agaaaaagtg tatgattatg atgtctaata ttagtataat
     5461 atatgcatag agaagtctaa atctaggata gatttttatt aaaagtaatt aaaattaata
     5521 aggatattat ttacagttat aagatagggt atttacataa ttaataataa ggatattact
     5581 agaatttcaa tttctagtaa tatttttttg cgtataaatg taaacttgac gaaataagta
     5641 gtataatcaa tataaattac atcatgcaaa ggaggataat ttggtggaga atgtttttaa
     5701 gcgattgcag gaatttaatg gttatgatgg atacaaagaa tcatttgaaa tgaattacct
     5761 atgtatatat gaaagtatac cgttaagaga acaagtagag cttgcaaata atctagtaga
     5821 tgaaatttta aatatgtata aaagtgaatc taatgaaatt tatttattgg aggatagtaa
     5881 ttctaagtct ttaatttgtt atttcgaaat ttttatgaaa aagataaata ctttggtaaa
     5941 agaaatgatt attgatgaga aatggttata taaattaact aaagaattaa tatataagag
     6001 taaaaaagtt gagtatgtaa aactagggtt agttttaagt gagaaatatc taaatgtaga
     6061 aaacttaaga gaagttgtag atacattttc taaatctgga gaatacgtat tttatctgag
     6121 taatactatt aaaaaacttg aattttacaa tacatattta tttaatttat ctaaaaaggc
     6181 tacaggttca ataaaagtat ttgctattgt taatatggaa aatttagatt ctaaaataaa
     6241 ttcctatcta attgaagatg gttataagga tacaaagtac gaacgattat tgatgaacta
     6301 tattatttca attgtagatt tgaatgaata tcttgaaaaa cgagatttag ataaagaaaa
     6361 gataaataat ttagcacgtc ttatatgcaa ttacttgcta agtgtagaat ttaaatatat
     6421 aggtaataaa ttagaattaa tgaatcgatt tttacctact gtagtaaatt atgggactaa
     6481 ctttgaaagt ttatattcaa ttttcctaat agctataaac gtcttaaaag atgaaaatat
     6541 agagtgcaat aagattgaat ttgaaaaaga aataaatgat atcttgctta gtgaaaaatg
     6601 gaaaaatata tattttgaag catcaagaga tgctagtgga aaaacagaag atattataaa
     6661 aatgtcagaa atttatgatg tgaacttatc ttttgatgac ttgttgccat atctgaatag
     6721 agatattaga gattttgagg tatattggca tatatctaaa aagggaacga caagttctag
     6781 attaaaatta ttaaattttt ttgaagagac gtttaaaata gatgatttaa ttggaaaaat
     6841 gaaagatata gaaaaagata aattgactca agaatattat gatgatatgt tattttttat
     6901 tgtgcttaaa ggaagtaaat cactatatcc tgaaggaaag aatatatctt taaaaggaat
     6961 atttggaaat ataaatgaag taagaaaaga atccataaac atcttaaaaa gatacagaga
     7021 gaaactatct tttgaagaat taaaaattgt taaagaagcg tatgaaaaag aaaaaaatgt
     7081 tatattgaag gatgaattaa gaagagtatt atatgaaagt aataatttaa aaaaagaatt
     7141 tgtaaatatt gaaaaaataa aagtagatga acatggtaag gatatctatt tgacttcaat
     7201 agctgttgct ggttcaaggt ttagaaatag agaatatctt gaaaaagaat tggagaaaag
     7261 taagatatac tatttaacta gagagaaaga taatctatat gatgaaaaag ctattaaaat
     7321 agttggtgaa actggatatg taataggcta tgtcccaaga aaagaaaact atattttgag
     7381 caatttatta gatggaggaa aactgctgta ttgtagagta actgaatata atttatatga
     7441 agattgtatc tacgcaaatg tgtacttgag ttataaagat gtaattgaaa cagttgaaaa
     7501 ctctttaaag atggttttag ataagagtag aataaaactt ataaactaag acgtttatat
     7561 attgcgtaga tggtgtaatc ctaactgatt ggttattttt tctggaatta tggtgtattt
     7621 tatgagcaat gctaatgaat ataaaataaa aaaacaattg ttttgtatat atgatgtata
     7681 taaatatatt tatatattca tatttacaaa acaattgaaa tttatgttat tttataattt
     7741 taaatttaga tggaatttta acttctgctt tatcttttat tttagaagct tgttttttaa
     7801 ctttatttaa taattcagat tcatctacaa ctaatacttt tctttccttc atcaaccatt
     7861 taccatcaca aatagtagag tctacatttt cactagtcat agaataaact atatttccaa
     7921 ttggattatg cattggtaaa caatgaattg tatctggatt tagaataatc atatcagctt
     7981 tttttcctac ttcaagacta ccaatttctt tttctagtaa agcacatttt gctccattta
     8041 tagtagccat ttctaagact tcttcagctg gtacgacagt tgggtttaaa gttcttcctt
     8101 tatgtattag tgatgtaaga tacatgtccc tcataaggtc cattctatta tttgaaggag
     8161 ctccatcagt accaattgaa acaggtattc ctttttctaa catttcagga attgatgcaa
     8221 atcctagaac tactttcatg gctgcagctg gattatgaga aacttttaca tcatgtaatc
     8281 taaataaatc aatttcttta tgagtaagcc aaactgtgtg agcagctaaa agatttggtc
     8341 ctaatacacc tagtttatca agatattcaa ctgttccaac tccattgttt tgctttacaa
     8401 atgaaatttc atcagctatt tcagcaatat gcatatgtat acctgtattg tatttatcag
     8461 ctagttcctt agttttaaca agtaattctt cagttgtatt aaaaatagtt cttaatgcaa
     8521 accatatttt aatcctctca ttagatgtgt tattaaactt atggaataag tctaattgag
     8581 tgttgatttc ctcatcagta gttttttgcc atgcttcagg aagaccatct cctgtatcca
     8641 taactgattt agctaaacag gctctaagtc cagttttatc aacagcttca accatagcat
     8701 ctacatattg accaccagat tctaaaaatg tagtgacccc agattttatc aattcaacac
     8761 aacatgcagt agatgaaatc aatgaatcgt catagttgaa gctactttcg tatggccaga
     8821 ttctctctct aagccaagtt aaaagtacaa catcatctgc aagaccacgt cctaattgtt
     8881 gggataagtg aacgtgtgta ttgactaacc ctggcattag tatttttcct ttagcatcat
     8941 atatctctgc atctttatct acaatattaa tatcaaactt accaacattt tttataatat
     9001 tattttcaat aagtatactt ccagtttcat atacctctct atctgcattc atagtaatta
     9061 gatatgcatt ttttataagt aattgtgaca taagtacaac ctcttttcta tatattttta
     9121 tatctatgta ttatgataat aaattttcta tacaaagtta atagcaaaat caatttatac
     9181 ataaattgat tttgctattt ataaatttaa aataaataag attttatttt atataattta
     9241 aaatattgac ctagaactat ttaaaatatt aacttagaac taagtttaca actgcaagta
     9301 aacctataat ccaaagtgta atactgattt tatcagtttc tttagctacc acatgcatta
     9361 ttatatatga aataaatcca aagcataaac caatacttat actataagtt aaaggcatca
     9421 ataaaactgt aacaaaagca ggtacagcta ttttgctatc agcaaaatca atctgtttta
     9481 tgctttcaaa cataaacaca ccaaccatta caagtgcagg agctgcagcg taagtaggaa
     9541 ctataccaag taatggtgta aatataagtg tagctaagaa taatgctgct gttactattg
     9601 aagcaagtcc tgttcttgca cctgctgcaa taccagaagc agattctcca aatgttgtaa
     9661 cagtactagt tcctaagaat gagcctatta aagtagaaga aacatctgca tataacattc
     9721 ttcctaaatc ttttatttca ccattctcat cttcaagacc catttgttta caacaagtaa
     9781 taagaaatgc taatgaatca aacatgtcta taaacatgaa agagaatata ggtccaatta
     9841 atgagaattt caatgcacct acaatatcta atttaaaagc aactggtgct atactaggag
     9901 gtgttgagat tattgctgtt ggcaactcca caaatccaaa tataacagat attattgttg
     9961 caactactat acttatcaat attccacctt taactttttt atattctagt attgccatta
    10021 agataagagc aaaaagagct attaaaactg ttggtgtaaa atctgcaagt gaaactatag
    10081 tatctggatt agctattata acacccatat tttttaaacc aacaaaagca aggaaaagac
    10141 ctatacctgc tgaagatgct actgccaatt ctctaggaat tgcgtctgca attgtctttc
    10201 gaagacctgt tactgcgagt gtaaagaaga cacatccaga tagaaaaact accccaagac
    10261 ctgtttgcca tgggattcct gcacctataa ccaaagagta tgtgaaaaat gcatttaatc
    10321 ccataccagg agctagagca aatggtgcat ttcctaaaat cgcaaatata agtgtaccaa
    10381 tggctgtagc tagtatggta actgttataa gggcggcttt atccattcca gcttcagata
    10441 atattgctgg atttacgaaa actatgtatg ccattgttaa gaatgtagta actccaccta
    10501 ttacttctgt tttaaggcta gagccacgtt cgctaaattt aaacattttg tctaaaaagc
    10561 ttaatgatga attatttcca cctggttgta cattgttagt gtttggtacc atttttacct
    10621 ccaaaaaact ttaagatata aacaaaaaaa tatatattca tatagataga atagaacata
    10681 tattcagagt tgacattcta ttcatagaaa acccatttaa ggtgagtttg tagaaactat
    10741 tagaccgtat tactaatatt atatgaacat tttctatttg tttttctgtt gtattatatc
    10801 gtaatattgt attatttaca ataaatataa taaaattatt cgtttttttg tcgattttat
    10861 aaaaaattaa ggttatttat tgaaaaaaat tactttttcc aataattgaa tatgtatgag
    10921 tataatgtca attattctaa cttgttttat actattaatg gaagtttatt ataaatgagt
    10981 ttgtgatata attataaaag tactgaatac atggatattt cgtaaaaact ttttaatata
    11041 tcaaatatgt atattaagtt aatatattaa aaaaaataat atcaaaaata atataattta
    11101 tagtggaggg gaaaatgact aaaattatta aagtgcaaaa acctaaattt acaggagaat
    11161 tagaaattat tgaaaatctt gaagatatat ttaataatga aaaaatattt aataaaatta
    11221 tagaaaatga agttataagt gatttagatt ctgtaagagt tagctttgaa tcttgtatat
    11281 ttagaaatgt atcctttgaa aattgcaatt taaaaaagat tgatttactt gatgtggtat
    11341 ttgaaaactg caatttatca aattcatttt ttgatggagg aagtatatac agagttgaat
    11401 ttaaaaatag taaattgata ggagttagat ttgatgattg tatacttaaa aacgttttgt
    11461 tcaaggatgt tttaggtaag tattctaact tttcattctc taaatttaag atagtttcta
    11521 tcttagattc aaattttgaa aattctgtat ttcaagaagt aaaaggtgac aatctagtat
    11581 ttgagtatac aaacttaaaa aaagcaaact ttaatggaac taaactttct ggcatagatt
    11641 ttactactaa taatattgag ggaattgaga ttggtataga tgatattaga ggagcaattt
    11701 ttgatgtgtc acaagcaatt gatttaacga aattgatggg aattatcata aaatgaaaaa
    11761 aatattcata aaataaattg ggaaaacgtt aacaatgtgt gaatttttac cagattgaca
    11821 tttttctgaa aataaagtaa gattatctta atattcaaaa tattttcaaa gggggtaatt
    11881 tagtgtgagt aagcagatga gagatatttt tatagttggt tttgctttat tttctatgtt
    11941 ctttggtgca ggaaatctta tgtttcctcc atttttaggg atggaatccg gcaaagactg
    12001 gttaattcct cttattggat ttgttgttgc agatgctggt atagcaatgc ttgttattat
    12061 agcaacagca agatgtaagg gaagcatgga tgatgtactt aggagagctg gtaaagggtt
    12121 agcaagagta atgagtgtag ctgctctagc atgtttagca cttcttgtaa taccaagaac
    12181 ttgcgcaaca acatatgaaa tgggtattat gcctatattt ggagaccatg gtccagtagc
    12241 taaagctgta ttttcaataa tcttctttgg gctaacttta gcatttacta ttaggtcttc
    12301 taaggttata gacataatag gtaagtattt aactccagcg ttattattag tattatttgt
    12361 tttaatagta aagggtataa tttctccagc aggacctatg agtcctgagc atatgataga
    12421 taaaaatcta tttggtgaag gtatatcaca aggttatcaa acaatggacg ctctaggtgc
    12481 agcatctatg gcaactataa tgttgatgtc tataatagct aaaggatata catcagataa
    12541 agaccaaata ggaatgacta ttaaagctgg ttttgtagcc tgtgtatttc tagcagtagt
    12601 atatggtggt cttacttatt taggtgcaac agtttcaact ttatatgata ctagtatacc
    12661 tcaagcatct ttattagttg aaataactag tatattgtta tatggtcaag taggaaaggt
    12721 aattttaggt gtaattgtgg cacttgcctg tctgactact tctacaggtt taacagcatc
    12781 aatatcaact tattttgaag gaataagtaa ggcgagttat aaacaactgg ttataggtat
    12841 atgtgtagct agtatgctta tatcaaattt aggtgttgat tctataatag ccatatcagt
    12901 tccaatccta caaactatat atcctgtttt attagcaata gttgttatgg agttggctgg
    12961 taaacatata aagaatgata atgcttttaa aggtgcagca tatgttacat tagtgattag
    13021 tttattatca gctataaatg gaatgacagg agctgttcca tttatacaaa gcttgccact
    13081 tgcaggtttg ggctttaact gggtaatacc agctatattg ggtggaatag ttggtaactt
    13141 tataaaaagc aacaaacaag tagcataata atttgtaatt aatttaattt tataatatat
    13201 taatttttag tactatgggt aggtttaatc taaacctaat ctacccatag tactttttag
    13261 attggattat aatagcatgt atataatttt ttataatata atttttcttt gaagtatgat
    13321 aaaattagta atagtgataa gaatgtacaa atttaggagg ttttaattaa tgcttagaaa
    13381 aataacagca tttatgctat ccatatgttt aattattgga cttacaggtt gtagtttact
    13441 tgtaggcaag aaagataaag agaaaattat agatgatttt aaagttgcag tggtaactca
    13501 accattatct gaaaataaag tacaatacaa tatggtggaa gagatggcaa aagaatatga
    13561 ggaagagaat aaaattgata aagacaaaga tggtcaaact aaagttaaac aaactataaa
    13621 gcatgttgta ttgccagaga attttacttc taatatagat agtgctatta ataagatagt
    13681 aaagttggct gacgataaag aagttcaggc tattgtagtt tcaactgacc aagcagggct
    13741 tttacctgct ttacaaaagg taaaggaaaa gcgtccagaa ataataacta tttctgcccc
    13801 tatgggagat gataaaaacc aattaagcca atttgttgat gtgaatctag gggttagtgc
    13861 agaagaaaga ggaaaagtat tagctgaacg ttcaaaagaa atgggagcaa aagcttttat
    13921 tcattatgcc tctacagatg atttgaaaga tgttaatata gccaaaagat tagaaatgat
    13981 taaagagact tgcaaaaata taggattgcc atttgtacaa gttaatactc caaatataaa
    14041 tactgaagaa gacaaaaaca aagttaagca gtttttaaat gaagatattg aaaaacaagt
    14101 taaaaaatat ggaaaagata taaatgtatt tggtgtaaat gagtatatgg atgaagttat
    14161 attgactaag gctttagaat taaagtatat agttgcagag caaagcaatc catcaccaat
    14221 acaaacatat cctagtgtta tgggtctaaa aatatctgaa aaagatgctc aaaattatga
    14281 taaaataaac gacatgataa gtgaaaaggc taaggctttt ggtatgtcaa atagacttgg
    14341 tggatatcct atgccaatgg atgcttttct accatcatta gcaatttatc ttgctactga
    14401 aatggtgaaa caagatttaa cacaagaaga tgtatgtgac ccagattatt tagaggcgtt
    14461 tacagaatta agatttggaa tcggttcaga atttactcct ttaacagagg tactttataa
    14521 ttatcaatca gtaattttaa gccaattaat ttattaatat taaggaagtg tctcaaaatg
    14581 aactttttag tttatgaggc actctttttc tattaaatca aatatatttt t
//
LOCUS       DAEMHW010000090        14623 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEMHW010000090 DAEMHW010000000
VERSION     DAEMHW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.090"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1..369
                     /locus_tag="KPK67_002244"
                     /pseudo
     CDS             1..369
                     /locus_tag="KPK67_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
     gene            1363..2175
                     /locus_tag="KPK67_002245"
                     /pseudo
     CDS             1363..2175
                     /locus_tag="KPK67_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
     gene            2178..2384
                     /locus_tag="KPK67_002246"
     CDS             2178..2384
                     /locus_tag="KPK67_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBG2459208.1"
                     /translation="MIRLTVEETNLLSIYNEGSKCELMENINAALPFMDEDMRELAKR
                     TLAKIVPLTENEFAELTIFDADEV"
     gene            2381..2746
                     /locus_tag="KPK67_002247"
     CDS             2381..2746
                     /locus_tag="KPK67_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBG2459209.1"
                     /translation="MTGVKPLTPPQSRKVNSLVKKECCNCDNGHCILLDDGEECVCPQ
                     LISYSLLCKWFQTAVLPLDKLLYAELFKTEDKKRCTECGASFASTSNSVKYCPDCRKR
                     ITRRQAAERMRKRRALVTQ"
     gene            3147..3848
                     /locus_tag="KPK67_002248"
     CDS             3147..3848
                     /locus_tag="KPK67_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG2459210.1"
                     /translation="MSIILKTTNLCKSFSKQTAVNNISLNIEKNSVYGLLGPNGAGKS
                     TTLKMITGILKPTSGSIEFDGHAWKRSDLNHIGALIEMPPLYENLTAFENLKVRSTLL
                     GLTDKRIEEVLQIVRLTETGRKKAGQFSLGMKQRLGIAIALLNSPKLLILDEPTNGLD
                     PVGIEELRELIRSFPAKGITVILSSHILSEVQQTADHIGIIAGGVLGYEGKLNANDNL
                     EQLFMDVVKNNHREE"
     gene            3852..4571
                     /locus_tag="KPK67_002249"
     CDS             3852..4571
                     /locus_tag="KPK67_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005347157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG2459211.1"
                     /translation="MNYLKSEHLKFKRTISNKLLFIIPLITAIFAWIVGGFIGFQYTT
                     LYWWYAFLLPGAIAILCSLSHRKEESAGKYYSVFSMPLNLSKFEIAKGIILIEKLLVA
                     GIFLALLISISNIISPATAVYSVPQSMVGSIAIVLASVWQIPLCLYLTRKTGLFVPIM
                     LNTVLGIFLPIVLGNTAIWWLVPYCWAAKLAEPLMGIELNGTFAGNSGFSITVFISVA
                     LSIFLFVALSFVDAKDFSKRR"
     gene            4575..5306
                     /locus_tag="KPK67_002250"
     CDS             4575..5306
                     /locus_tag="KPK67_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005347158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG2459212.1"
                     /translation="MGFWGAVHSELVKVKHTPFWTIHLCLPVVGALLFVVYYILYGNT
                     ADYKKLKMILELTAIVFPLLISVVVSLNIVLEEKASHFQTLLGVPNRYKVMLTKLVVL
                     YGIGITALFCLFLIFLLGVHFVGIANTIQLSMLVKAAAGMAFYNLIIYALHLFLGFRF
                     GLGISLFWGVFESLQCILYSNIELNSMGRYIPFAWSMNWVHDVMNGRLSAHATEWIWI
                     AVLTIGGVLLTLLWFSHWEGRKNYE"
     gene            5299..6108
                     /locus_tag="KPK67_002251"
     CDS             5299..6108
                     /locus_tag="KPK67_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NisI/SpaI family lantibiotic immunity
                     lipoprotein"
                     /protein_id="HBG2459213.1"
                     /translation="MNKKRIIFVLFAFIVCAAFTGCSLQDKIKEYSSDKEQCYLNTEN
                     VTQFSYKGNDYTILADTVSNGGLGEWIGYIRQLAAIDENGKILLQENVETATFQSLAD
                     LAEKAPEAAYIIPFLNVYAAPNADNYLIVDVNGGYHKAVISENVKDSDTIFDFKETEK
                     SINGSFEVNPENATQLLCGVTVYQVTADMVSDDELGSYIDILAKNVTFDTETKKPLSK
                     EDLSSIDWYGENDGQGREQWIYTDIYEIYGIDKSEAVAVKINNHYYIAKRQ"
     gene            6170..6829
                     /locus_tag="KPK67_002252"
     CDS             6170..6829
                     /locus_tag="KPK67_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2459214.1"
                     /translation="MAAILIVDDERSILELVKNGLQKDGHSVTTYTSATQVPLDKLNK
                     YDLIILDIMMPDIDGFSYCDKIRSLVDCPILFLTAKTMENDITFGLGLGADDYLTKPF
                     RIAELRARVNAHLRREHRERHTALTFDRIKIDLSAKELRIDNIPVPLTKSEYLICEYL
                     AKNKGQVFSKEQIYEAVFSLEGDSDNSTISTHVKNIRAKLNKLDIQPITTVWGIGYKW
                     D"
     gene            6820..8184
                     /locus_tag="KPK67_002253"
     CDS             6820..8184
                     /locus_tag="KPK67_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2459215.1"
                     /translation="MGLKKIISLKASFWKFLCMLLIGLICSVAIPFSFIYIGVGTNFI
                     TYADYSERSTQNLVPIIAATPELTDVQFPMGCKYLVLDKNYQMLETSLEGDDLDRAMD
                     YAISGKTSVNLNKQYLLVTRENEYVVLQYYIGSQFTNEWLNEHFPSPEILLYILIGAN
                     CIAVCVILTTKFSKNLRVQLAPLFEATEQVAQQNLDFEVGHSKIKEFEDALCSFANMK
                     DNLKMSLEKQWHAEQLQREQIAALAHDLKTPLTVIQGNIDLINETELDDEQRLYADYI
                     TESSGQIGIYIKTLIDISRTVSGYQLHLEKFDISDYMEQIEAQAHSLCLTKGICLHME
                     TGANLGVLKADKLLLERAIMNVISNSLDYSPPQGTIYVTAKKTDSFLHISITDEGSGF
                     TPEAIHHAQEQFFMGDKSRTSNMHFGMGLYITSSIIKQHDGQLILSNSKKTGGAQVII
                     KIPY"
     gene            complement(8247..8870)
                     /locus_tag="KPK67_002254"
     CDS             complement(8247..8870)
                     /locus_tag="KPK67_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBG2459216.1"
                     /translation="MKSILLHGLGQTSSDWDKTVEVMENRMDIICPNLSEWLVDKEPC
                     YNTLYQMLENYCNQYNEPLNLCGLSLGGILAMQYGIEHADRVNSLVLIGTQYTMPKRL
                     LRFQNMLFHIMPKSVFSQIGFQKKDFISICSSMIELDFQQNLRDIQCPVLVLCGSKDN
                     ANKTASVDMEKMIPCAKLSIIQNAGHEVNKDNPAELGKQLNTFFNLK"
     gene            complement(8942..10285)
                     /locus_tag="KPK67_002255"
     CDS             complement(8942..10285)
                     /locus_tag="KPK67_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBG2459217.1"
                     /translation="MAVTKIKPIKSTLSKALDYIQNPDKTDGKMLVSSFGCSYETADI
                     EFGFTLAQAIEKGNNLAHHLIQSFEPGEVDYEKAHEIGKQLADAVTKGQHEYVLTTHI
                     DKGHIHNHIIFCAVNFVDYHKYNSNKRSYYGIRNMNDKLCRENGLSVVVPGKGSNGKS
                     YAEYQAEKTGTSWKGKLKIAVDTLILQVASFEELLQRLQAAGYEIKQGKYISCRAPGQ
                     ERFTRLKTLGTDYTEEAIKERIAGKRTKAAKAPKEQRGVSLLIDIENSIKAQESRGYE
                     QWAKIHNLKLAAKTMNFLTEHKIEQYADLVSRIDEISAENVKTADALKRVEKRLADMA
                     VLMKHVTTYQKTKPVYEAYRRAKDKDAYRAKQESSLILHEAAAKALKTTGITKLPNLI
                     AMQEEYGKLQVQKEALYADYGKLKKQVKEYDVIKQNIDSILRQVKEPERGKETER"
     gene            complement(10246..10575)
                     /gene="mobC"
                     /locus_tag="KPK67_002256"
                     /pseudo
     CDS             complement(10246..10575)
                     /gene="mobC"
                     /locus_tag="KPK67_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610348.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
     gene            complement(10819..11184)
                     /locus_tag="KPK67_002257"
     CDS             complement(10819..11184)
                     /locus_tag="KPK67_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2459218.1"
                     /translation="MIMKQNKEKFDFKAFGQAIKAARKAKGISRNQLADTLNIAPRYI
                     ASIENSGQHPSLQILYELVTLLDVSVDQFFFPEREQEKFTRRRQLDTMLDTMSETDLK
                     IMSATAKGIEEANNDRTGE"
     gene            11490..11745
                     /locus_tag="KPK67_002258"
                     /pseudo
     CDS             11490..11745
                     /locus_tag="KPK67_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728674.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11882..12589
                     /locus_tag="KPK67_002259"
     CDS             11882..12589
                     /locus_tag="KPK67_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG2459219.1"
                     /translation="MCTRFVYNGNDMITGFNFDIDLAVWKHKVIKEKERFYIGILRPD
                     GTYHSYHGINRNGNVGTLLYVHDNPAGIYRDNSDCITIADLTEQFIKAQISFDEVLQI
                     VKSKQITYAPDATMQAMLSDSHGRVLIIEPGIGYKEEYEQYSLITNYSLMKPESTKDF
                     IVPGDDRYERALKKLEKYDPDFSISDAIHLLYDVRQEGAWATRVSFVYSAKEQTVYYV
                     ENNHFECIQTFTFPLER"
     gene            12603..13222
                     /locus_tag="KPK67_002260"
                     /pseudo
     CDS             12603..13222
                     /locus_tag="KPK67_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728672.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
     gene            13685..14125
                     /locus_tag="KPK67_002261"
     CDS             13685..14125
                     /locus_tag="KPK67_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2459220.1"
                     /translation="MTPNRREFEKQCAFQKFCKSVLHNEACNAHEEIRRRRVREVTFS
                     NLALHEERQLYTVDKYFQDEEAEPSYQMAGKEITPKLLLEAIRALPEERRKIVLLYYF
                     EGLTDIEIGKQLNISRSTVQYRRTSSFEQLKKYLEENADEWIEW"
     gene            14106..14363
                     /locus_tag="KPK67_002262"
     CDS             14106..14363
                     /locus_tag="KPK67_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2459221.1"
                     /translation="MNGLNGNQNDYPENALVPYPVILAASKGDPDAMKIVLQHFSGYI
                     ASLSMRKLYDERGNVYFGVDEEIRERLQAKLMRAILTFSAE"
BASE COUNT         4397 a         2918 c         3153 g         4155 t
ORIGIN      
        1 atgccggaac gccccgaccg ccgcacccgc gaggtacagg cagaaagcgt cgcctatgct
       61 gtctgccaac attacgggct tgatacatcg gattattctt ttgggtatat cgccgggtgg
      121 agcagtggaa aagaacttgc agaactgaaa ggctctcttg aaacaatccg cagtaccgtc
      181 gcaagtctga ttgataccat agacgggcat tttgcagaaa tccaaaaggc acaggataag
      241 gaacagacaa ccgaacaggc gcagcccgca caggaagccg ccaaacagcc cgaaacagaa
      301 acagccgcgc aggagcttcc cgaagaaaca gccccggcac aggaaaaaga agcacagccg
      361 gaacaggctg cacccaccgc cccttattac actatcaatg aagctgccgc caaacgtgca
      421 aaggatatga acagtttttc cgattataag caaggcagcg cgacggctga atacaggcat
      481 tatgttgatg aagccgtaca aatcgcagaa aggcaaaaac agcgggtaga cccgatgtac
      541 catgagaaaa tcgacagcct gcttgatacc tacgcccgga aactggcggc gaacatgaac
      601 aaaggctatg agattgacgc gcgtgttccc tctatcctga ttgcgggcgg ctctaacttc
      661 cccacaagga agaaagaaaa gcagaacgca gcccgcgaca gcaattaccg ggaatggcag
      721 gacatacaag ggcttcttga taaaatccgc agtaccggca tgggcggtat cagcgcagac
      781 gacccgcagg cagtacagaa gttagaaaag aaactggaaa gccttgaaaa atcacaggaa
      841 accatgaaag ccgtaaacgc ctattaccgt aagcataaga cccttgacgg ctgcccgcat
      901 ttaccgccgg aggaactgga aaaactgaaa gcagacatgg cgagcagttg gcatttggag
      961 gacaagccct ttgcgacttg ggcgttatcc aacaacagcg cggaaatccg gcgcgtgaaa
     1021 gaccgtatca aatccctttc acagcaaaaa gaaatcggtt ttgtggattg ggaatttgac
     1081 ggcggcacgg tggaagcaaa caccgaagcg aaccgactgc aaatcttctt tgaggataag
     1141 ccggacgaag ccacgcggga agccttaaaa agtaatggct tccgttggtc gccaaaagcg
     1201 ggggcatggc agcggcagct taccagtaac gcttactatg cggctgatta tgtcaaggcg
     1261 attgcacctc ttaccgggga aaagcctacc gagttacagc gggcgcatat ccggcagcaa
     1321 aaggtagctg tacaagaaca acccgcacag gagcagccgg aacaggaagc ccaacaggac
     1381 aaagacacct tttccattta tcagataaaa ggcggggacg aaacaaggga cttgcgcttt
     1441 gaaccttacg accgtctgac tgccacagga caccgggtag accaaaaaaa ctatgcgctt
     1501 gtctattccg cagagcttac gccggggact tcccttgaag atatttatac ccgctttggt
     1561 atcgaccacc cgaaagattt taagggacac agcctttccg tttccgacgt ggtggtactt
     1621 catcagaacg ggcaggacac cgcccactat gtagacagtt tcggttataa ggaagtgccg
     1681 gagtttttca aggagcagga aaaagccctt attcccgaca acttggaaac gggcgaaaca
     1741 atcaaaacac cgaggggggc tttctatgta accgccatga gccgcgagca aatggaagct
     1801 gccggatatg gattgcacca ccagtccgac gacggaaaat atctgattat ggggaatggc
     1861 acaagggctt ttgctgttcc cgttcagccg gaaaactatc tgaaagctgc cgagcagacc
     1921 accgagcaaa attacaatat gattgacggg cagataaata acaccccgac cgtcgcagaa
     1981 ctggaagcaa aggtaaaggc aggagaaacc atttcccttg ttgaccttgc ggaagctgtc
     2041 aaggctgaca aggaacgcgg caaggcagaa aaaacggaaa agaagccctc tatccgagcg
     2101 cagcttagag cagacaaaga aaagaccgca cagaaaaaag cggcaaaatc aaaaaatcac
     2161 gaattggagg tatgagcatg ataagactga ccgttgaaga aacaaacctg ttgagcattt
     2221 acaatgaggg cagcaaatgt gagcttatgg agaacatcaa cgctgcgctg ccgttcatgg
     2281 acgaggatat gcgggaactg gcgaagcgca cccttgcgaa aatcgtacct ctgacagaaa
     2341 atgagtttgc ggagcttacc attttcgacg ctgatgaagt atgactgggg ttaagccgct
     2401 tacgccgccc caaagccgga aagtcaacag ccttgtaaaa aaggagtgct gcaactgcga
     2461 taacggacac tgtattttac tggacgacgg ggaggaatgt gtctgcccgc agcttatttc
     2521 ctattcgctt ctttgcaaat ggtttcagac cgccgtactt ccccttgata agctgctgta
     2581 tgcagagctt ttcaagaccg aagataaaaa gcgttgtacc gagtgcggag cttcttttgc
     2641 gtccacctct aacagcgtaa aatactgccc ggattgtcgg aagcggatta cccgcagaca
     2701 ggcagcggag cgcatgagga aacggcgcgc ccttgttacg cagtaggggc aaaaaaagcc
     2761 ttagtttaca aggcataaaa gacgcgaaac ttgcataagc gatacagaat accttttccc
     2821 ttaaaatcga ggttctactg cgtaacatat caaatcatct gcacccacaa aaaaacgggc
     2881 gcggcggcta tgactacatg gctgctgcgt ccgttttttg tgtccgatta tccccgttct
     2941 attccttttt ttcgctccga ctctttttgc taccgcaaaa tactgtttat gtttcttgat
     3001 aacttcatat tccttgacct gttttttcaa ctttaacccc caatattttc tattatgtat
     3061 tttgagaaat tctattttct ttatgaaatc ttcaaaattt gcggttatcc ttttagtgaa
     3121 aataaaaaga aaggatagta caaaatatga gtataatttt gaaaacaaca aatctctgta
     3181 agtcattcag caagcagaca gccgtaaata acatatcgct gaacattgaa aaaaactccg
     3241 tctatggatt gttaggaccc aacggagccg gaaaatctac aacactcaaa atgataacag
     3301 gcattttgaa gccgacatct ggaagtattg agtttgacgg acacgcatgg aaacgaagcg
     3361 acttaaatca tattggagca ttgattgaaa tgccgccgct ttatgagaat ttaaccgctt
     3421 ttgaaaatct gaaagttagg tcaacccttt taggactgac agacaaaagg attgaggaag
     3481 ttttacaaat tgtccggctt acagaaacag gcagaaaaaa agcaggacag ttttctcttg
     3541 gcatgaaaca aaggcttgga attgcgattg cattattaaa tagtccgaag ttgcttattc
     3601 ttgatgagcc tacaaacggt cttgacccag tgggaattga agaacttagg gagcttatcc
     3661 gttcctttcc ggcaaagggt attaccgtta ttttatccag tcatattctt tccgaggtgc
     3721 agcagacagc agaccatata ggcattattg caggcggcgt cttaggttat gagggaaaac
     3781 ttaatgcaaa tgataatttg gaacagcttt ttatggacgt tgtaaaaaat aaccataggg
     3841 aggagtaata tatgaactac ttaaaatcag aacatttgaa atttaaaagg acaatatcaa
     3901 acaagctgct tttcatcatt ccacttatca cagctatttt tgcatggata gtgggtgggt
     3961 ttatcggttt tcaatataca acgctttact ggtggtatgc gtttctgctt ccgggagcaa
     4021 tcgcaattct atgttcttta tcacaccgga aagaagaaag tgcagggaaa tattattctg
     4081 tattttctat gcccttgaac ctttcaaaat ttgaaatagc aaaaggaatt attctgattg
     4141 aaaagttact tgtagcagga atatttttag cattacttat ctcaatcagc aatatcattt
     4201 ccccggcaac agcggtatat tctgtcccac agagtatggt aggtagcata gccattgttc
     4261 ttgcgtccgt ctggcaaatt cctctatgct tgtaccttac gcgtaaaaca gggctgtttg
     4321 taccgataat gctaaatacc gtacttggaa tatttcttcc tatcgtgttg ggaaacacag
     4381 ccatttggtg gcttgtgcca tattgttggg cggcgaaact ggcagagccg cttatgggaa
     4441 ttgaactgaa tggaactttt gcaggaaatt ccggcttttc tataaccgtt tttatttctg
     4501 ttgcactgtc aatcttttta ttcgttgcat tatcttttgt ggacgcaaag gatttttcaa
     4561 aaaggaggta gagtatgggc ttttggggtg cggttcattc tgaattggta aaagtgaaac
     4621 atacgccatt ttggacgata catttatgtc tgcctgttgt gggagcgtta ctatttgttg
     4681 tttactatat cctttatgga aacacagcag attacaaaaa attaaaaatg atattggagc
     4741 ttacagcaat cgttttccct ttgctgataa gtgttgtcgt tagcttgaat atcgtattag
     4801 aagaaaaggc ttcccacttt caaacattgc ttggagtacc taaccgatat aaagttatgc
     4861 ttacaaaatt ggtcgtttta tatggaatag gaataactgc actgttttgt ctatttctta
     4921 tctttcttct tggcgtccac tttgttggaa tagcaaatac tatacaactt agtatgttag
     4981 tcaaagcagc cgcaggaatg gcattttaca atttaattat ttacgccctg catttgttcc
     5041 ttggctttcg gtttggattg ggtatctcat tgttttgggg agtatttgaa agtctgcaat
     5101 gtattttata tagcaatatt gagttaaata gcatggggcg atatattccc tttgcatggt
     5161 ctatgaactg ggtgcatgat gttatgaacg gcaggctttc tgctcatgca acagaatgga
     5221 tatggattgc tgtattaaca ataggaggtg tattattgac tttattatgg ttttctcatt
     5281 gggaggggcg aaaaaattat gaataaaaaa cggattatat ttgtactgtt tgcttttatc
     5341 gtttgtgctg cttttacggg ctgctcttta caggataaaa tcaaagaata ttccagtgac
     5401 aaagaacaat gctatctgaa tacagaaaat gtaacacaat tttcctataa aggtaatgat
     5461 tatacaattt tggcagatac cgtatcgaat ggtgggcttg gagaatggat tggatatatc
     5521 cggcagcttg cagccataga tgaaaacggg aaaatattac ttcaagaaaa tgttgaaacg
     5581 gctacttttc aatctttagc ggacttggca gaaaaagcac cagaagccgc gtatatcatt
     5641 ccttttttga atgtatatgc agctcctaac gcagacaatt atctgattgt agatgtaaat
     5701 ggtgggtatc ataaagctgt tatcagcgag aatgtaaagg atagtgatac gatttttgat
     5761 tttaaggaaa cagaaaaatc tataaacggc agctttgaag tcaacccgga aaatgcaaca
     5821 cagctccttt gtggtgtgac ggtttatcaa gtaacggctg atatggtatc agatgatgaa
     5881 ttaggcagtt acattgatat tcttgcaaaa aatgttacat tcgacactga aacgaaaaaa
     5941 cctctatcga aagaggattt aagtagtatt gactggtatg gggaaaatga cggacaagga
     6001 cgtgagcagt ggatttacac agatatttat gagatttatg gtattgataa aagcgaagca
     6061 gtcgcagtca aaataaacaa tcactattat attgcgaaac gacaatgata acttttctgc
     6121 ttttttgaag tatgctatac taaaaggtag aatgaaagga ggcgataata tggcagcgat
     6181 tcttattgtt gatgatgaac gttccatttt agagcttgta aaaaatggat tgcaaaaaga
     6241 tggacattcc gttacgacct atacatctgc cacacaagtt cctcttgata agctgaacaa
     6301 atacgacttg attatattag acattatgat gccggacata gacggttttt cttattgtga
     6361 taaaattcgt tcattagtgg attgtcctat tctctttctg acagcaaaga caatggaaaa
     6421 tgatattacg tttgggcttg gtttaggtgc tgacgactat ctgactaagc cgttccgtat
     6481 tgcagaactg cgggcaaggg taaacgccca tttacgccga gaacataggg aaagacatac
     6541 tgcgcttact tttgaccgga taaagattga tttgtccgca aaggaattac ggatagacaa
     6601 tatacctgtt ccattaacca aaagtgaata tctgatatgt gaatatcttg caaagaataa
     6661 gggacaggta ttttcaaaag aacaaattta tgaagcagtt ttcagtttag agggcgacag
     6721 tgataattct acaatttcca ctcacgtcaa aaatattcgg gcaaaattga ataaattgga
     6781 cattcagccg ataacgacag tttggggtat tgggtataaa tgggattaaa gaaaataata
     6841 tcattgaaag cgtctttttg gaaattttta tgtatgttac ttattgggtt aatatgttcg
     6901 gtagctattc cgtttagctt tatttatatt ggagttggta caaattttat tacctacgct
     6961 gattattcag aacgcagtac ccaaaatctt gttccgatta ttgcagcaac tcctgaattg
     7021 acagatgttc aatttcctat ggggtgcaaa tatttagtgt tggataaaaa ttatcagatg
     7081 ttagaaacat ctttggaggg cgacgattta gaccgagcta tggattatgc tatatcgggg
     7141 aaaacaagtg taaatcttaa taagcagtat ttacttgtta ctcgcgaaaa tgaatatgtg
     7201 gtgcttcaat attatattgg ctcacaattt acaaatgaat ggttaaatga gcattttcct
     7261 tcgccggaaa ttttgttgta tatcctcata ggcgcgaatt gcattgcggt ttgcgtaata
     7321 ctaaccacaa aattctctaa aaacttgaga gtgcagcttg cccccctttt tgaagcgaca
     7381 gaacaggtcg cacagcaaaa tcttgatttt gaagtgggac attcaaaaat caaagaattt
     7441 gaggacgcac tttgttcttt tgctaatatg aaagataatc tgaaaatgtc tttagaaaag
     7501 caatggcacg cagaacaatt acaaagagaa cagattgcag cactcgctca cgatttgaaa
     7561 acacctttga ccgttattca aggaaatatt gatttgataa atgaaacgga acttgatgac
     7621 gagcaacgtc tatatgcaga ttacatcact gaaagctccg gtcaaatagg tatttatatt
     7681 aagacgctaa ttgacatttc ccggacagtt tccgggtatc agcttcattt ggaaaaattt
     7741 gatatttctg attacatgga acagattgaa gcacaggcac actccctatg ccttacaaaa
     7801 ggaatttgtc tgcacatgga aacgggagct aacctgggcg tactcaaagc ggataaactg
     7861 ctgttagaac gggcaattat gaatgtgata agcaattcgt tagactactc tccaccacag
     7921 gggacaattt atgtaacagc gaaaaaaaca gacagctttt tgcatatatc cataacagac
     7981 gagggaagcg gtttcacgcc agaagctata catcacgcac aggaacagtt ttttatgggc
     8041 gacaaaagcc gaacttctaa tatgcacttt ggtatgggac tttatatcac aagcagcatt
     8101 atcaaacaac atgacgggca gcttattttg agcaattcta aaaaaactgg cggcgcacag
     8161 gttataatta aaattccata ttaaaaatgc actcacaaaa gacgggcgcg gcggctatgg
     8221 ttacatggct gtctgcgcct acttttttat ttgagattga aaaatgtatt taattgtttt
     8281 cctaattcag cagggttgtc tttgtttact tcatgtcctg cattttgaat aatagataac
     8341 tttgcacaag gtatcatttt ttccatatcc acagaagcag ttttatttgc attatcttta
     8401 ctcccgcaca aaaccaacac tggacattga atatctctca aattttgttg aaaatctaat
     8461 tctatcatag aactacatat actgataaaa tccttttttt gaaaaccaat ttgtgaaaat
     8521 acagacttgg gcattatgtg aaacaacata ttttgaaatc ttaataaccg tttaggcata
     8581 gtatattgtg ttcctatcag caccaacgaa tttaccctat cagcgtgttc aattccatat
     8641 tgcatagcga gaataccacc caaagaaaga ccgcacaaat tcaagggttc attgtactgg
     8701 ttacaataat tttctaacat ttgatatagg gtattataac agggttcttt atctacaagc
     8761 cactccgaca aattcgggca aataatatcc attctatttt ccataacttc aaccgtttta
     8821 tcccaatctg aagaagtttg ccctaaacca tgaagtaata ttgatttcat taaactatcc
     8881 tctttctttt ttgtaagcga acataaggta aagttattat agcataccat gtcggcttat
     8941 gctatcgttc tgtttccttt ccccgttccg gctctttgac ctgccgcaaa atgctatcta
     9001 tgttctgctt gataacgtcg tattccttga cctgtttttt cagcttgcca taatcagcgt
     9061 aaagggcttc tttctgcact tggagtttcc cgtattcttc ctgcatggcg ataaggttgg
     9121 ggagcttagt aatgcccgtc gttttcagtg cctttgccgc cgcttcatgg agtatcaaac
     9181 tgctttcctg ctttgcccgg tatgcgtcct tatcctttgc tctgcggtat gcttcataaa
     9241 ctggttttgt cttttggtag gtggtaacgt gcttcatcag caccgccata tcagcaagac
     9301 gcttctccac gcgctttaat gcgtctgctg tctttacatt ttccgcactt atttcgtcaa
     9361 tccggctgac taaatccgcg tactgctcaa tcttatgttc ggtcaagaaa ttcatagttt
     9421 ttgcagccag tttcagatta tggattttcg cccactgttc atagcccctg ctctcttgtg
     9481 ccttgatact gttttcaatg tcgataagca gcgatacgcc gcgctgctct ttgggagctt
     9541 tcgccgcctt tgtgcgcttg cccgcaatcc gttctttgat tgcttcctct gtataatctg
     9601 taccgagggt ttttaagcgg gtaaaccgtt cctgtcccgg agcgcggcag gagatatatt
     9661 ttccctgctt tatctcatag cccgctgcct gtaaccgctg caacaattct tcaaaacttg
     9721 ctacttgcag gataagcgta tcaacagcaa ttttcagctt gcctttccaa cttgttcccg
     9781 tcttttccgc ttgatattcc gcatagctct ttccattgct gcctttgccc ggaacgacaa
     9841 cagacagccc gttttcccgg cacagcttat cgttcatgtt ccgtatgcca tagtagctgc
     9901 gcttattaga attgtactta tggtagtcaa caaaattgac tgcacaaaaa atgatatggt
     9961 tatggatatg tcctttgtct atgtgggtag ttaagacata ttcatgctgt ccctttgtta
    10021 ccgcgtcggc aagctgtttt ccgatttcgt gggctttctc atagtctact tctcccggct
    10081 caaaggactg tatcaaatgg tgggcgagat tgttcccttt ctctatggct tgtgcaagcg
    10141 taaatccaaa ctcaatgtct gctgtttcat agctgcaccc aaaggaggac acaagcattt
    10201 tcccgtctgt tttatccggg ttttggatat agtcaagggc tttgctcaac gtgcttttaa
    10261 taggcttaat ctttgtaacc gccatatttc tgcaagcacc ccctttattt cctctatgtc
    10321 ctcttggtag acgctgcctg tcgcattgac acgctttgca atctgattga tgttgacccc
    10381 gattttctat atctctgctg tcatggcttt tatgtcgcta tggtctgtct gaatgatata
    10441 cccgtcaatg gcaatcttgc gaagatacgc cgccatgttc cgggtgggga cgagcttcat
    10501 tttctgctca atcaatgccc tttcttcctc tgttacatag aatttgattt gtatgtttct
    10561 tttgcgtccg ttcatgcaat cgctcctttc cattccgagg gtttgggaca cttcccaaca
    10621 agcaattttc aaccgacgcg ccgcaggaca ggagggcgaa aatacggaaa agggtactct
    10681 tttcctatgc ttgctaagaa agttttttcc tcacttatca ctacaacaaa actcccagta
    10741 tgccaaaaaa gagcaaaaga aaaacgccgc aatcctgcga cgcttcaatc actttatgca
    10801 gacaaaaacg agggaacttt attcccccgt cctgtcgttg ttggcttctt ctatgccttt
    10861 tgcagtagca gacatgattt ttaagtccgt ttcgctcata gtgtcaagca tggtatcgag
    10921 ctgacggcgg cgtgtgaact tctcctgttc ccgttccggg aaaaagaatt ggtctactga
    10981 tacgtcaagc agggttacaa gctcatacaa aatctgtaaa cttgggtgct gcccgctgtt
    11041 ctcaatggac gcgatatatc ggggcgcaat attcagcgta tcggctaact gattgcgtga
    11101 tattcccttt gcttttcttg ctgctttaat ggcttgcccg aaagccttaa aatcgaattt
    11161 ttctttattc tgtttcataa tcatttcacc cagttacatt ttactgttcc tcttttcttt
    11221 gagatatgat tacacagaac atagtattga ctgaaataga gcatattata ttaaaagcag
    11281 cgttgtcttt ttgagatgaa aatgttatac tgacaataat ctctattttt attgaaagaa
    11341 gtgatttata tgtgtatcaa cacaaaaaag agaaacaaag attttctcca tgcaaagcca
    11401 gtagattgcg acggttgcat ggagtaaaat agtcgcagaa taaatgctgg ctttgtaact
    11461 tgactatcat aatcaaggag gaagcctgca tgaaatataa aaatgcacaa cttattcttc
    11521 cggacgcatt ggtaaaagag ctacaaagtt atgttcaagg cgaatatatt tatgttcctg
    11581 tcgagcagga gcagcagggg agaagtttcc ggttacagga aagaattaga gcaacgtaat
    11641 cagaagataa aacaagaata tcaaagcggt atttctataa attgtcttgc cgaaaaatat
    11701 tgcctatctt tatatgctgt tcggaaaatt atatatcaaa agtaaggtaa ggggctgccg
    11761 agtgtgcagc ctcttatttt tatacagatt ggttcggtat tatatagagt atagaaaaat
    11821 ttttcaacga actgtatgat aaagaagaaa tatttgaagt acatgggagg agggaactat
    11881 tatgtgcacc cgatttgttt ataatgggaa tgacatgatt acaggattta attttgatat
    11941 tgaccttgca gtatggaaac ataaagtgat aaaagaaaaa gaacgctttt atataggtat
    12001 tttacgaccg gacggtacat accactctta tcatggtatc aatagaaatg gaaatgttgg
    12061 aacattgtta tatgtacatg acaatcctgc cggaatatat cgagataatt ctgactgtat
    12121 aacgattgca gacttaacag aacagtttat taaagcgcaa atttcttttg atgaggtact
    12181 ccaaattgta aagtcaaagc aaattaccta tgcgccggac gcaacaatgc aggcaatgtt
    12241 atcagacagt catggacgtg tcttaatcat tgaaccggga ataggctata aagaagaata
    12301 cgaacaatat tccttgataa cgaactattc tttgatgaag ccggaaagta caaaagattt
    12361 tattgttccg ggggacgata gatatgaacg tgctttgaaa aaattagaaa aatacgaccc
    12421 tgatttttca atttcggacg caattcattt actttatgat gtacgtcaag agggcgcatg
    12481 ggcaacaaga gtgtcttttg tgtattctgc aaaagaacaa accgtatatt atgtagaaaa
    12541 taaccacttt gaatgtattc aaacattcac ttttccgtta gagagataaa ggaggagcca
    12601 ttatgtcaca gaaaatttat ccacgttcaa aagataaaga aacggtatat cttaaaagtg
    12661 ttatcactaa ccctaatatc atagtgggcg attatactat gtataatgat tttgtgaaag
    12721 aaccaaaaga ctttgaaaaa aataatgttt tatatcagta ccctatcaat caagacaaat
    12781 tgataattgg gaaattttgt tcaatcgcat gtggagcaaa gtttgttttc aatagtgcaa
    12841 atcatacatt atcttcctta tcaacatacc catttcccat ttttttgaag aatggggatt
    12901 ggacgtaaaa gacattacaa aggcatggga caataaaaga gatattgttg tcggaaatga
    12961 tgtttggatt ggttatgaag cagtcatttt agcgggtgtt acgattggcg acggagctat
    13021 tatcggagca cgagccgttg taacgaaaga tgttccacct tatacaatcg tgggaggtgt
    13081 acctgcaaaa cctatacgaa aaagatttaa tgaggaaacg attgatatat tgctcaaatt
    13141 aaaatggtgg gattggtcta aagagagaat agctaaaaat attgctgcta ttcaatccgg
    13201 taatgtgaaa caactaaact aaatctgccg cgcgacggca aaagaaaaaa agccgtcgca
    13261 atgcagacat attttaatgc gttttttcag cggtggtttt gaatttcaga gccgccgctt
    13321 ctttttgcat aaatttagca acgacgaccc catttgacct tgcaatcagc atggcggtgt
    13381 ataggaggtt gccgttatgc tgaataaaat aagttatgct tatctgacaa aagaacgtct
    13441 tttgtcaaaa aaagtttgta tcctacaacg ggttattccg gctatgtaga tgaactgtca
    13501 aatcaattaa atactgctgt tatttcctat gcgtctgtct gcattttgca ggcaggcgca
    13561 tttcgcattt tacagaagat ttttcaaaaa ctttgctctc tttttggcaa agcaaaaagt
    13621 cgcctgtatt atcccgcgaa aaggggaggt actaccgcct ttcggcagaa aggaggtgaa
    13681 caccatgaca cctaatcgca gggaatttga aaagcaatgt gcgtttcaaa agttctgtaa
    13741 atcagtgctg cacaatgaag cgtgcaacgc ccatgaggaa attcgtcggc gcagagttcg
    13801 ggaggttact ttttccaacc ttgccttgca tgaggaacgc cagctttata ctgtcgataa
    13861 gtattttcaa gacgaagaag cagagccgtc ttatcaaatg gcaggaaaag aaataacccc
    13921 aaaactgctg cttgaagcta tccgggcttt acccgaagaa cggcgcaaaa ttgtactgct
    13981 gtattacttt gagggattga ctgatataga aatcggaaag cagctcaata tctcaagaag
    14041 tacggtacag tatcgccgga caagttcctt tgagcagtta aaaaaatatt tggaggaaaa
    14101 cgctgatgaa tggattgaat ggtaatcaaa acgattaccc ggaaaatgcc cttgttccct
    14161 atcccgttat tttggcggcg agcaaaggcg acccggacgc aatgaagatt gtattgcagc
    14221 atttcagcgg ctacatagca agtctttcca tgcggaagct ctatgatgag cgaggaaatg
    14281 tttattttgg agtagacgaa gaaatacgcg aaaggctgca agcaaaactg atgagggcaa
    14341 tcctcacatt tagtgctgaa taagccaacg cggtattgga acgcttcccc cttttccgtt
    14401 ccatatcgca gaacatccct ctgctctttg acaaagaaag cggcgcaata gaacggcgac
    14461 gcagcataag gcaaatcgga tacgttcctt ttgtgtcgag ccatgcggcg ggtacgccat
    14521 gacctttctc ttaatagaaa agcgagcgag aaataccaca ccgtaaagca gggatagcag
    14581 cctgcgggcg ataacgcaca gaatacataa tgatacttcc tta
//
LOCUS       DAEMHW010000091        14528 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEMHW010000091 DAEMHW010000000
VERSION     DAEMHW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.091"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      complement(207..290)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(523..1365)
                     /locus_tag="KPK67_002263"
     CDS             complement(523..1365)
                     /locus_tag="KPK67_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2459222.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(1617..3401)
                     /gene="pepF"
                     /locus_tag="KPK67_002264"
     CDS             complement(1617..3401)
                     /gene="pepF"
                     /locus_tag="KPK67_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG2459223.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3467..4132)
                     /locus_tag="KPK67_002265"
     CDS             complement(3467..4132)
                     /locus_tag="KPK67_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG2459224.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(4283..4444)
                     /locus_tag="KPK67_002266"
     CDS             complement(4283..4444)
                     /locus_tag="KPK67_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBG2459225.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(4522..5031)
                     /locus_tag="KPK67_002267"
     CDS             complement(4522..5031)
                     /locus_tag="KPK67_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBG2459226.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            5237..5455
                     /locus_tag="KPK67_002268"
     CDS             5237..5455
                     /locus_tag="KPK67_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2459227.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5473..7419
                     /gene="feoB"
                     /locus_tag="KPK67_002269"
     CDS             5473..7419
                     /gene="feoB"
                     /locus_tag="KPK67_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG2459228.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQNKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(7579..8634)
                     /locus_tag="KPK67_002270"
     CDS             complement(7579..8634)
                     /locus_tag="KPK67_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2459229.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(8682..9482)
                     /locus_tag="KPK67_002271"
     CDS             complement(8682..9482)
                     /locus_tag="KPK67_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2459230.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(9475..10266)
                     /locus_tag="KPK67_002272"
     CDS             complement(9475..10266)
                     /locus_tag="KPK67_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2459231.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(10306..10728)
                     /locus_tag="KPK67_002273"
     CDS             complement(10306..10728)
                     /locus_tag="KPK67_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBG2459232.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(10744..11223)
                     /locus_tag="KPK67_002274"
     CDS             complement(10744..11223)
                     /locus_tag="KPK67_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2459233.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIGIEFRMLPRDKKKQLSDLIK"
     gene            complement(11355..14111)
                     /locus_tag="KPK67_002275"
     CDS             complement(11355..14111)
                     /locus_tag="KPK67_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2459234.1"
                     /translation="MRDEILSFVNECALFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         4728 a         2499 c         1632 g         5669 t
ORIGIN      
        1 ctgcatctca atgtttataa aaactttatt ttcttattat aatactatta ttctttttaa
       61 aaaatttccc ttttccaaca aatggctact ttaaaagcca aaatttcacc tatcaataaa
      121 aatcttaacg actaatactc ttattttcaa attttgcaac atttttgtta attttaaaac
      181 aaaaaaagac tatgcaatat aaaaattata ttcatagccg gttgcaccac taactccata
      241 tatctctaga tgcccaataa taagatatac ttctcagttt ctaaaatatt tttattaaaa
      301 atcttttata tattttcata aatttctttt ttattctaac attgtaatat tattttgtca
      361 atatttccat attcttagat ataagatact aaaataccta ttttatctaa agatatttat
      421 atttctacat tcaataatag acaataccct gcaagttaaa gcatcatttt tatgagctta
      481 tctcacaggg tattatatgt ttactttata ttataactct tgttattgat taactttaat
      541 aaatcctttt tctgaaacaa gtgcttgtcc ttcatcactt aatatataat ctattaaagc
      601 tttacctttt tctgacaaac catcttcttt attaactaat ataaatggtc ttgatattgg
      661 atatttatta gctattacat ttgcatcagt tgcttcaaca tcatttattt ttaaactatt
      721 tactttctca tcaacatatc caaatgatat ataccctata gcattttcat ttccttcaac
      781 tgtagttttt atatttcctg aaccatcact tatttgtgca tctttagtta gttcttctga
      841 atcaaaccct acaatttctt ggaatccatc tctagttcct gaaccttctt ctcttgatac
      901 tattactatt ggtgcatcat ttcctcctac ttctttccag ttagtaatct ttcctgtata
      961 tataccttta acttggtcca ttgtcagatt ttttactgag tttgcagtat tagttatcaa
     1021 tgctatacca tctattgcta ttttagtttc ctttaatcca gcactttttt cttcatcttt
     1081 taaatctctt gaactcattc ctatttcaga tgttccttct atagcatttt ttatacctcc
     1141 agaagaacca atctgttgta cttctacact aacaccttga tttttttctt gaaacttttc
     1201 tccgatagtt tccataagag gacctattga agttgaacct gaaacagaaa ctttgcttga
     1261 atctcctgaa tcacctgaac ctcctgagtt agaactacat cctactgcta aagctccaac
     1321 taacattgta ctagctaaaa gaacgcctat tttcttttta aacattttac ttacccccgc
     1381 ttatatttta attttttgtt gtcatttttc tatattttaa taatacagat tctttgttaa
     1441 atgaatatta gagcagggtt aagtaaatgt aaatattgtt aaatacatgt aaaaacttaa
     1501 ttttatatga tttattctta attagattaa ctttttaaag ttactttagg cagatataaa
     1561 cataatacaa ctcatattat cagaagtctt cattgaagct atcaacctat cacatcctat
     1621 aattcttttt ctagtttatt aactaattca gcaaatacat ttaaggcttc ctctatagat
     1681 tcttttttcg tcatatctac accagcagat tttaattgtt ctagtgggta ttctgaccct
     1741 ccacttttta agaagtttat atatctatct actgcagtac ttccttctga aagtatctgt
     1801 tgacttaaag cacttgcagc agaaaaccct gtggcatact tatatacata gaagtttgaa
     1861 taaaaatgag gtattctcgc ccattctatt cctatttgtt tatctactat tgcagatttt
     1921 ccgtaatact tttcatttaa tttgtagtat atattagtaa attcatcagc agtaagtggt
     1981 tctccactct ctaccctttg atgtacaatt ttttcaaatt cagcaaacat agtttgtcta
     2041 tgaactgtag ttctaaattg ttctaaataa taatttaata agtatatagt ttcatcctta
     2101 gacttagaat tttctaataa ataatttata agaagtaatt catttaatgt agatgcaact
     2161 tctgcaacga atatcttata gtccgaatat aaaaatggtt gacttgttct tgaataatga
     2221 ctgtgaacag agtgccctaa ttcatgaatt aaagtgaata gagaattaag gtcattttta
     2281 tagctcatta atatataagg atgtgaatca taacttcccc aagaataagc tcctcctttt
     2341 ttaccttcat tatcatatac atcaatccac ccttcatcaa aagcactttt tatgaccttc
     2401 aagtattctt ctcctaaagg tttaagtgcc tctaatatga tatcctgtgc tttatcatat
     2461 ggtatatcca tgtcaaattt atctgtcaaa ggaacataca aatcatacat gtgtatttca
     2521 ttcaatccta aaaatttctt tttaagctca acatatttat ttagattagg aatactttta
     2581 tctatagctg atataagatt attgtaaaca tctaaactta catcatctga aaataaagac
     2641 gcttctattg ctgaattata ctttctagtt tttgcgtaaa atatctctga ttttattcca
     2701 ccataaagcg ttgaggcaaa tgtattttta tatttctcat atgttgaaaa ttcaccttca
     2761 aaagcgtctt ttctaactct actattctta ctttttataa ataaagagta gtttgaatga
     2821 gaaagcttaa ccttattccc ctcctcatct tctatctcag gaaattccat atctgcatat
     2881 gctaacatat cgtatacatt ttctggaatt gtcgataatt ctgatgtagc tgcaagtatt
     2941 tcttcctctc gttcactcaa agtatgtggt ttatctctca atatatcctt tatatatttt
     3001 gtataaaagg atagcttctc atcttttaag tattcactta acttattctc ttctatcgcc
     3061 agtatttctg gaactatata agaagtagcc acacctaaat cagtagataa catttctgtc
     3121 ttagtggctc ttccttgatt atcattaatt ctagtatctt catgttgttt catatgagta
     3181 tatacatata aattttgaac aatcctagat gcattttcag agatatttaa tactttatat
     3241 aagttctcct tactctcaga aagcttccct ttatattctt ttacttcttg gataagattt
     3301 tttactttac ttatgtcttt ttcaatttcc tctttactgg agtacatctt atctatcttc
     3361 catttgtact tttcttctat tgtacttcta tcagttccca tatcatacac ctctatttta
     3421 atttgtttta tattttattc actttacaaa atatattgta tatattttag tccaaataat
     3481 ctacatttat tactccttct atcatattta tttctttcat aacgtcgtgt aagtgtattc
     3541 tcttagcata agatacaaaa aaattaacct tccagtattc ttcattacga cattctattt
     3601 ccatatttct tacgataagt tgattttttt caaatttatc atataaaaaa ggtatagagt
     3661 ccaaattgct taattttaat atcaatacat tcttttcttg gctaaataaa aacgtattga
     3721 ttatcctaag cacaaataat gctatcatta caaataatcc acctagaatt gccaattcat
     3781 aataaccata tccaactgct ataccaatac atgcagttgc ccacatccca gcagctgtag
     3841 taagcccttt tactccacct ttatttttta aaattgctcc tgcacctaaa aaaccaattc
     3901 cagataatac ttgagcaggc attcttgctg ggtcagcatt tgtatacaaa ttataatcaa
     3961 agaataattg aatcgaagtt attgaaacaa tacaagaacc tattgcaatt agtatatgtg
     4021 tcctaaatcc tgcaaactgt cttatttttt ctctctctaa gcctattatt cctcctatta
     4081 ttagtgctaa tagtaacctt attgctatct ccttacttcc tatatcaatc ataatataaa
     4141 accctcctga cttaattaca acttatgtcc tacagataat ttacacatca gaaatactat
     4201 tttatatctt aatattatta ttaatatatg taaaatatag ccatctgtta atttttgata
     4261 caaaaaaacc cacaactgtg ggctatttgc ttaggtacat gtatttattt attaatatat
     4321 ctattttctt acttgattct actaactctt cttgaaatat ttctggatat tctatgtatg
     4381 tatttatctc ttccctaact gcttcaatct cattttttaa cgcttctaat tcattgttcg
     4441 ccataataat caccactatt acaaatttgt atatttttat atacatttta tcaaacaata
     4501 ttatattttg tcaatattat gtcaataaaa cttactataa aaatgcttta atttaggtat
     4561 tgtatacctg gatactaagc ctacaaacat accagttact aatgatgcca ttaacataaa
     4621 tggtaagtac cctatagcaa gtatattttc tatgattaaa cttgcaacta ttaattgacc
     4681 tatattatgc cctattgcac ctataatgct tatccctatt agtgataaat cttttatttt
     4741 actcgctaaa tacataaata aaagactcag ataagctcct cctatactaa ataatagata
     4801 tgataaaggg cctgcaaata ttgatgacaa aattaccctg atagtaacaa ttataacagt
     4861 atctttgaca cctaacaaca ttaatgaaat taaagttata atattgctca aacccaattt
     4921 tgcccctgga aatataacta taagaggatt tggtatatat gtctctaata catataaagc
     4981 taaactatac cctatcataa gacctaaaaa tgtcattttt ctagttttca ttaagttaac
     5041 tcctccaaat tttatctatc atcttacatt ataatacaaa atatataaaa acacctaatt
     5101 tagactaaaa tatttttaat ccatttagaa taaatattag ttatttcaat gtgcaattac
     5161 ctctataaat cttgactttt tgtatcatac ttgttacaat tttaatgata atgaatttca
     5221 ttaggaggtg ttaataatga ctgtttacga tttaaaaata ggagaaaaag gatttattaa
     5281 tagtatagat ggagatatta aattatctaa aagattgctt gcacttggat gtataaatag
     5341 tactgaaata gaagttaaaa aggttgcccc acttggagac ccaattataa ttagatttag
     5401 aggctttgac cttgcgatta gaaaatctga tgctaaaaat atttctttac aataatagag
     5461 gaggagctac ccgtgattaa tgtagcatta ttaggaaacc caaatgtcgg taaaactact
     5521 gtttttaatc tacttacagg ttcaaatcaa tatgtaggta actggcctgg tgttactata
     5581 gaaaaaaaag aaggatttct tggaaaagag ataaaggttg tcgatttacc tggtatatat
     5641 gcaatggata ctttctcaaa tgaagaaaag gtttctaaat catatttaga aaatgaagat
     5701 gttgatgtaa tagtaaatgt agttgatgct tctaatttat caagaaacct ttacttaaca
     5761 actcaactta tgcaatttaa caagcctata gttatattgc ttaatatgtt agacattgca
     5821 gaatctaaag gagttaatat agatgccaaa aaattaagtg aagaacttgg agtaattgtt
     5881 gttccaatta tagcaaaaaa gaaaaatggt gttgatagga tagaagaaga gattaaggga
     5941 gctacgcaaa aagcttttat atatcagaaa aactttggaa gtgaaacaga aacatataaa
     6001 aaattagaat ctattttatc gagatgtaca aaaactacat atacacaaaa taaatctatc
     6061 agtgataaga tagacaatat agtcttgaat cctatattag catatccgat atttattggt
     6121 gtattgttcc tcttatttaa atttaccttt gactgggtcg gaggaccact acaagaggga
     6181 tttgctagtc ttatagaggc ttatatttct gcacctgtta gtgatatgtt atctaactct
     6241 agcccatggt ttaaatcact tatagttgat ggaataattg gaggtgttgg tggtacttta
     6301 ccattcttcc ctttaatatt tacattattc tttggtatat cgttgtttga agatagtgga
     6361 tacatgtcaa gaacagcatt tttaatggat aaagttatga gaaaggttgg tctatctggt
     6421 aaagctttta tacctatagt tatgggtatg ggatgttctt ctccagctat aatggcaact
     6481 agaacattgg aaagcgaaaa agatagaaga gttacagctc ttattgcacc acttatgact
     6541 tgtggagcaa agctacctat atatgcacta tttgtagcta tattctttcc acataatgct
     6601 gctttagtaa ctacatcact gtatttggtg ggtatagtta tggcaattgt agttgcatta
     6661 ttcttaaata aaacagcctt taaaacagag gctgaaccat ttatattaga attaccagaa
     6721 tatagaatcc ctactataga tgcacttatt aaaaatactt ggaataaatc aaaaggtttc
     6781 ttgataagag ttgttactgt catgtttgca atgtcagtcg taatttgggg actttcttca
     6841 ttcaatgttt ttggatatac agaagatata aatgtaagct tcttggctac tctaggtcat
     6901 attatatctc ctatattcaa acctcttggt tttgatgatt ggagaacttc agttgctatt
     6961 ttaagtggtc ttggagcaaa agaaatcgta gtaaactctt taaatatact atatggtaat
     7021 ctaactgttg tcttaccaac aatatttaat ggagttactg catatacatt cttgatattt
     7081 actgctttat atactccatg tatagctgca cttgcaacta tgaaaaaaga atatggcaat
     7141 aaaatgatgt ttacatcatt tgcatatcaa tttatactag catggataat ggcttttata
     7201 gttaaaagta taggtggagt actatttatg ggtaattcaa taatagaagc tttgataggt
     7261 ggaataatta tagtccttgc actctttatt ctgtttaata aattcaagtc tacaaaaaac
     7321 ggaaatgtct gtagtggatg ttctatttgt tctggatgtc caagtgctaa ttcttgctca
     7381 agtaaggaag acagcaataa aaaaaataat gcacactaat atataaatat tttagtttaa
     7441 aataagataa aagtgggtgt ctcaaaatta actttttagt tcatgaggca ctctttttta
     7501 tataaaatca aatatatttt catcatttca acagtaaaaa agaggcttat ctctattttg
     7561 agacaacctc tttttttgtt acttattcag tttcttgcta cctaacttca tgtggaacct
     7621 agtatctttt ggaacacttg ggtctacacc tcttaatgta ggtatttccc cacatattag
     7681 ttgtataggt actataaaat taaatgtttg atagtaagga ttatctgtga tatcaatctt
     7741 taaattttta tcattttctg taacatctct acctatagca aatacatttt ccgtccaacc
     7801 agaaagtaca tcaatcatct tagttactct aggttctttt cctgtatcta gtataaatat
     7861 agtagaatca ctatttatag cattatatat accatgtata aactcttcaa actcataccc
     7921 agttacaggt attctcattg tttccaataa ttttaatgct gcttctaatg tatctccata
     7981 tatatcagag tgacctatta ttcttatttc tttagaatta actaactttt ctttgtttct
     8041 ttcaatccat tgtttagaga gcttatacac tgcttcaaat ctattaattg cgtctaatat
     8101 cttatttatt tcctcattgt acttttcact agatattata cctttttctc tagctatttg
     8161 aagacctaat aacattaagt ttaactttgt acaataatat ccttttgttt tagctccaga
     8221 tttttcttca ccacaattta ctgttaatat ataatcagaa atctcatcta ttaaagcatt
     8281 cttacaacct gccatagatg ctattttaca gcccttatct tcagctaact tcatagcgtt
     8341 gtatgtagaa taactactcc caccttgaga tacacctact actagggtat tttcattatc
     8401 aaacttgaat gtatcttcag ttatcataaa tggatacatt ttcacaacat ctatatctaa
     8461 taaattttgt aaatatggtt gcacttgcac tcctgagtga tagctagtac ctgaacctgt
     8521 tattataatc ttttttagat ttgttctctt aacttcatta aataagctat ctgcattaga
     8581 tataatttct ctcattctaa ctggtgtttc taacatataa tcttgtattg tcattttata
     8641 caccttcctt atctatatat tcaataaatt ataattaatt tctataacaa acctatagca
     8701 tgtaaagcga ttcctgttac tatagtgcct atagttaatt ttgttacact tacctttctc
     8761 tttaacatgt agtatattac aaatgcaaag gttaaaggta acatattagg tattatttta
     8821 tctagtatat cttgtaaaat tacttctgca ccatttaagt tgaatttcag aggagtagtt
     8881 atactcaaca tagttgcaac cattcctcct acaacgaata aaccaacagt tgtagccata
     8941 gacattattt tctccataag tccctctctt tgtattcttt caagagaatt tacccctact
     9001 tgataaccat acttaagtcc aaatattcta agggcaaatg ctggtatatt gtataatagt
     9061 aagaataata gtggtccaaa gatattacct tcttttgcaa gtgatactcc tactcctgct
     9121 gctataatcc taaatgttcc ccagaataat gaatcaccaa ttcctgcaag gggtcccata
     9181 agagctgttt taacagcact aattgaactt tcatcaaatt ctggattatt agcattctct
     9241 tcttccatag cggctgttat acctaatata gcaggaacta ccactgtcgt tgtattaaaa
     9301 atctccaaat gcctttctat tgcttttgct tgattttctt tcttattgta tagcttcttt
     9361 attgcaggta acattgaata acagtaacct aagttttgca ttctttcgta attaaatgca
     9421 ccttgaagag caaatgaacg ccaaaacata cttcttaatt ctttttttgt taatttattc
     9481 aaagtcaatc tctccttccg ttaaagtatt gattgctaat tgaggatttt gattattttc
     9541 tttgttattg atataaaagt ttaatacaaa tgctacacat actccaaata aagctatagc
     9601 agttatatct aatccaccat aagatgctaa caaaaaccct ataaagaaaa atacagacat
     9661 cttttttgaa agtaacatat ccataagcaa tgcaaaacct actgctggca acaatgttcc
     9721 tgcaacaccc agtccgtcta atataacttt aggtattgca tttattaatg ctgtaacttt
     9781 atcagcacca atcataattg ctaaaaaggt tggaactgat gtacttaaaa agaataatat
     9841 tactggcaac cacatcatta tggtaacttt cttaaaatca gcctgtttag catagaaaac
     9901 tgccttttga gaaaagtacg aatttattat tctaactaat actccaagag attgtgctaa
     9961 gactgctact ggtactgcta ctgctaaagc aacttctgca ccattacctg ataatatagc
    10021 aaaagctgta cctaatacac ctcccgttac tacatctgga ggagtagctg cacctattcc
    10081 tgcaattccc atccaaatta attcaagctg tgcaccaatc ataattcctg attttatgtc
    10141 tcctaaaact aatcctacta acatacctgt aactattggc ctatcaagca tagattgacc
    10201 aaatatacgc ccatctagta taccaactcc tgctataata gctaacatag tagcttgtac
    10261 taacatattg aacctccatt ttaacttagt ttaatttaca ttaaattaca ttacattttt
    10321 gattttgttt tgattttcta ataactcatt tacataaaca attccatttt ttgcttcctc
    10381 taatcctgat ttaactatta gttttgtatc ctcctcaact aataaaagta tagataaaag
    10441 cattggtaaa tttactcctg tcaataattc aaatttgaac ccttgtaaaa gtaagtttgt
    10501 acatatgttt gccacacttc caccaaacac atctgttaag acaacaaatt cttcactatc
    10561 tttattattt gataatatac ttctaatttc tttctcagca tcatctgctg atttatccgt
    10621 tgtcatgcaa aaatactcaa tattagttaa tgaaccaatt attaatttac ttgtgtcaac
    10681 aagctctctg gacaattctc catgtgttgc aattagaaat tttttcattc tctttctcct
    10741 aaattatttt attaaatctg atagttgttt ctttttgtct ctaggtaaca ttctaaattc
    10801 tataccaatc cctaaatcac ctatttgttt aaagttattt atatcatcat catcaacagc
    10861 aactaaatct gtaagtttct tcttcccttc ttcatatctt aatcctccta catttatttc
    10921 ttttaatact cctcctgaat ttttagctac aactaaagca tctttggtat tttttactaa
    10981 cactaataca ttgctttttt ttgaagaagc aaatttttct actatttctc cagattcctc
    11041 tacccccctt atatataaag ttacattgct aggtttcgct aagttcagtg acatagcttt
    11101 catttggtca ttttttgctt catcatttgc aactagtatt acatttatac ctagtgatgt
    11161 tgcccatcca tatgcaacct gaccatgtat caatctgtca tcaattctca tcaaagatac
    11221 cattttcccc aaatcctcca taatacatta aaatttttat tgtttatact ctcatttatc
    11281 tataaaagca aaaagtatgc caaatactgg catacttttt taaatttcat tcacttattt
    11341 tgtaatcttg tattttactg tggtagacca tagataatat tataaatata tcctatctct
    11401 gaaattggta tttgcacact ataactcttt tctagcacac taaatgcatt ttttattagt
    11461 tttacttcac tatgatggtt tttgattaac aaatccatat cagtatactc ttcaatttca
    11521 gtttgtctaa ctaatctttc aatcatacaa cttaagtgaa catataaaga aatcttttta
    11581 tcatttgaaa gttttttctt ttgaatattt tctatagcat taattccttc ttctattctc
    11641 tctattatct tttttgaatc taaaatagtt agactatcta ttactctaat taatgaaaaa
    11701 ttctttacaa tagaatcatt tatgttcttt attttttcat tttctactac tctgctaaat
    11761 attctaaaaa catcatcttc tccttttcct gatattatat cctctaataa tataaaatct
    11821 accccattca catttggatt ggcagttcct actatagcca tcacatcata catttcaaaa
    11881 actggttctt tatctcccat agaaacaaga ctatcataat cataaggtat tattttgaca
    11941 tcaactattc caattaaact ttcggaaagt agatgttgga tttttatagc agtacctatc
    12001 cctgtaaaac aagatgatat tatagcatat tctttatttt tttgaggata atacaattgt
    12061 atattaggtt tagtttcttc atccataatt tctgcaatct cttcaatgtt tttaccttgc
    12121 attaataaat ttcctacttc tagtgcagaa atagtggata tttggtctat aaataacatt
    12181 gggctattta tcttattctt taaatcttca tatataagat taagagagcc catatcaaat
    12241 aatacaatta aaccattctt tatcctatct ccctctaggt atctagacat ttgttttgat
    12301 acatcagcaa tagtagcatc tataggcata tcaatagctg taaatatatt tttacccaac
    12361 atccgattac atacattcgc catactactg gcagtagcat atccatgtgc tataatagtc
    12421 gcattaactt gattaaattt cattcttata attgttgatt taataaatat aatcattatg
    12481 attgtatcta cattattaag atacatatcc aatctattcg ttattaatgt tgatatttta
    12541 cttattatag agtattcttt gtaactctct ttttttaaat atattagtaa tttattttct
    12601 aaagctttat actcatcaga caattgataa gattcgtcct tcaaataaag ataagacgaa
    12661 aatgccaata cattattccc atcgtatttt atattgttat taacttctaa atttctaaat
    12721 atatcctgca ttatcgatat tataagctcc ataagtacgt tattattttc atcattattt
    12781 gaaaagacta aattgtccat tagtctaatc attagattag aactcttttt actaaatttt
    12841 tcctttgtta tttcattttt ttctagtcta tcaaagtaat ctaaacattt tatgaaactt
    12901 tgatagatta tatttctttt atttcttcca tttgacattt caatactaaa ttgtttagga
    12961 gaaaagataa tgcttttgct tttaccactt tggaattgaa atatatctac attttctata
    13021 taaagatttc taggaaggtc ttgaagagta acaaaaatat ttccatttct tctttgattt
    13081 tttgcataag ctcgtccaca tgcatatttt attacatttt tacattcacc tatattcccc
    13141 ttgaatttta tctttaaaat cgcatttaat gtaatttgag ttatctctat aggcaaacca
    13201 agtaccttac tttcttcata aataaaatgt tctacaatct ttcttttttc aatagtccct
    13261 ctttcttcta tattgggaac ataaatattt atgggtattc ttcttaaaaa ggtttgtaaa
    13321 aattcagata gactttctgt tgtagcaaat attaatctta ccctagcttt tctaattact
    13381 ccattctctc caatcctaga aaacgtacct gtatccataa atgtaaataa cttttcctga
    13441 ccttcactat ttaatctatg gacttcatct aaaaataaaa caccaccatc tgattgttct
    13501 attagacctg ctctagattc atttgctcca gtatatgctc cttttacatg cccaaataat
    13561 agactagaca ataactccaa attattataa tactgcgcac agtttaacac attgtatgga
    13621 gaattttttt ctaatacatt actgcttaca ctatatttgt atattatctt ggctaaaaaa
    13681 gtttttccta ctccactttc acctgtcagc attattgtaa gtccgccatt tggataaagt
    13741 acagcggttt ttatttgttc gattacttct tttaagctat tatcatatcc tatgatacta
    13801 ttaaacacag tctcttgctt taataattta cactcatttt taagttcatc taaattttca
    13861 taaatacttt tttttgtagg tcctactaca tcatttattt tatttttagg taaaaaaata
    13921 acaggtctac tgtttatctt tataagatta tcagatttta ctaaatcatt taataatcta
    13981 cttgattgag ttctacttaa attaaacttt tttactatat cttctgttat tatgccactt
    14041 tcttttttaa gttcatcaaa gttgttaaat aatgcacatt catttacaaa acttaaaatt
    14101 tcatccctca tgttcaacct ccaatacaaa tatcaataat actataatat tagtatatca
    14161 tttatatatt tatgaaaaag ataattatat tcctatcttg attctctttt gtaagattgt
    14221 aaaatcaaac tttttataag tataaaatct agatttttta acataatctc aaggttctta
    14281 tctttccata tatctgacat agaatttata taagaatata tttattgtat ttattataat
    14341 aaaaagtaaa acattatagg tattttaaaa cttattagcg aagaaaatat ttgtaacttt
    14401 tattacctta tgaatattat ttcaaattta aaaaggctat aaataaattt atttttaaat
    14461 aaatctactt ataacctcac aataatactt ataaatttta ttttttcaca atgatatatc
    14521 tcaaatat
//
LOCUS       DAEMHW010000092        14526 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEMHW010000092 DAEMHW010000000
VERSION     DAEMHW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14526
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.092"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            286..1299
                     /gene="aroF"
                     /locus_tag="KPK67_002276"
     CDS             286..1299
                     /gene="aroF"
                     /locus_tag="KPK67_002276"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG2459235.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKIVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1321..2385
                     /locus_tag="KPK67_002277"
     CDS             1321..2385
                     /locus_tag="KPK67_002277"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBG2459236.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2385..3698
                     /gene="aroA"
                     /locus_tag="KPK67_002278"
     CDS             2385..3698
                     /gene="aroA"
                     /locus_tag="KPK67_002278"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBG2459237.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGKTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3676..4755
                     /gene="aroC"
                     /locus_tag="KPK67_002279"
     CDS             3676..4755
                     /gene="aroC"
                     /locus_tag="KPK67_002279"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBG2459238.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4758..5954
                     /gene="pheA"
                     /locus_tag="KPK67_002280"
     CDS             4758..5954
                     /gene="pheA"
                     /locus_tag="KPK67_002280"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBG2459239.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDDEQKIEFSLDS
                     SNKKFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6001..6810
                     /gene="aroE"
                     /locus_tag="KPK67_002281"
     CDS             6001..6810
                     /gene="aroE"
                     /locus_tag="KPK67_002281"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG2459240.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6822..7355
                     /locus_tag="KPK67_002282"
     CDS             6822..7355
                     /locus_tag="KPK67_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBG2459241.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIMI
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7376..8215
                     /locus_tag="KPK67_002283"
     CDS             7376..8215
                     /locus_tag="KPK67_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBG2459242.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            8483..9964
                     /locus_tag="KPK67_002284"
     CDS             8483..9964
                     /locus_tag="KPK67_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG2459243.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            9997..13434
                     /locus_tag="KPK67_002285"
                     /pseudo
     CDS             9997..13434
                     /locus_tag="KPK67_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
     gene            13452..14198
                     /locus_tag="KPK67_002286"
     CDS             13452..14198
                     /locus_tag="KPK67_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBG2459244.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNKI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYVSKSKLSFVPTYYTREFFALWKTIIFDR"
BASE COUNT         6022 a         1433 c         2441 g         4630 t
ORIGIN      
        1 atgaaaggaa tattaaaaga actaacagca aaactttata gcttttttta ctttatgttc
       61 tggggctttt attttagcgc tggctatttc ttttacaaaa atatatgtac ttcctttctg
      121 gtgaggcctt attttagtga ttttgagcat aagtaaattt tataaaaatg attattttat
      181 atatttatat aagatttagt gcttgataga ttattttaga gaactcatta gtgagttctc
      241 ttttttattt taaatataaa attataatat tgggaggtag taaaaatgat agtagtatta
      301 aaaatgggag cagataaaaa tgaagttaaa aaattaattg aggctattgg gagagaagga
      361 gtagaagtta atcccataga tggaacagaa ttaactgttt taggtttagt tggagatact
      421 agtaaaatag atgccaaaag aatagaagca aataagatag ttgaaaaagt tatgcatgta
      481 gtagagcctt ttaaaaaggc caatagaaaa tttcatccag aaccttcaat cataaatgtt
      541 aatggaatgg aaattggttc aaagaaaatt gctatgatag ctgggccttg ttctgttgaa
      601 acagaagacc aaatagtatc tatagctaaa gatgtgaaaa aatcaggtgc aggatttttg
      661 agaggtggag cattcaaacc aagaacatca ccatacgctt tccaaggatt gaaatatgat
      721 ggattggatt tattaaaaaa agcaaaagaa aaaacaggac ttcctatagt tacagaaata
      781 atgtcaacac aagatataga tatatttgaa gaaaatgtag atgtaattca agttggagct
      841 agaaacatgc aaaactttga tttattaaaa gaattaggta aaacaaataa aacaatactt
      901 ttaaaaagag gattatctgc tacaattgaa gagtggctaa tgtcagcaga gtatataatg
      961 gctggaggaa atgaaaatgt agttctttgt gaaagaggta taagaacttt tgaaacatat
     1021 actagaaata ctttggattt aagtgcaatt ttagctgtga agaaacttag tcatttacca
     1081 gttatagttg acccaagtca tgcagcaggt aaatcatgga tggttgactc tttatctaag
     1141 gcagcaattg cagttggagc agatggtcta ataatcgaag ttcataatga cccagcacat
     1201 gcactttgtg atggaaaaca atcgattaag ccaaatgaat atgatgaatt gataagtgaa
     1261 ttaaagacta tcgcatcagc agttggtaga gaaatataat ctataaaagg atggttaaac
     1321 gtggaagaaa aactattagt aaatttaaaa gaaaatagct acaatatatt tattaaaaaa
     1381 aatatactaa agaatatagg agaagaaata aaaaaaatat attttggaaa taagattttt
     1441 attgtaactg atgaaaatat agcaaaatta tacttggata aagtaaaaaa taatttaagt
     1501 gaatctggat ttgaaattaa atctttaata ttaaaatctg gtgaagaaac aaaatcattt
     1561 aatactcttc ctaaaatata cgatagttta cttgacttta agttaactag aaaagatttg
     1621 attattactt taggaggagg ggtaataggt gatttaggtg gatttgcagc agcaactttt
     1681 ttaagaggta taagttttat tcaaattcca acatctttat tagcacaagt tgatagttct
     1741 gtaggtggaa aagttggagt tgaccttgag agaggcaaaa atttagtggg aagcttttac
     1801 caaccaaagg ctgtgtttat cgacccaaat gtattattat cacttccaga aaaattctat
     1861 agagatggaa tggcagaagt tataaaatat ggatgtataa aagacaaaac ttttttcaat
     1921 aaacttttaa gtttaaaaaa tagagaagaa gtaatgcaac atatagaata cattatacat
     1981 aaatgttgct ttattaaaaa aactgtagtt gaagctgatg aaaaagactt tggagataga
     2041 atgcttctaa actttggcca tacattaggt catgctattg agaaatacta caattttaca
     2101 ggttataccc atggagaagc tgttgccata ggaatgtata agataacttt agaaagtgaa
     2161 aagataggaa ttacaaaaaa agggacatca gaacttataa aatcaatttt aatgaattat
     2221 aatttgcctt atgagattga attaagagat aataaatcta tagtagatgc tatatctctt
     2281 gataaaaaaa atctaggtga tacattaaaa ataatacttt taaaagatat tggagagagt
     2341 attatatata atactacttc taaatttttt caggaggtaa gataatggga agtctaaaaa
     2401 tatatccaag taaattatct ggagatgtaa aaataccacc atcaaaaagt atggcacata
     2461 gggcagttat ttgttcatct ctaagcaatg gaaaaagtag aatttctaat atagattttt
     2521 ctgatgatat aatagctact ataagagcaa tgacatcatt aggagctatt atagaaaaga
     2581 aagaagatat tttagaaatt tcgggaattt tttctaaaga aggtatttta aatagagaga
     2641 atcagttgaa tcaacctaaa ttgacaatag attgtaatga atcaggctct acattaagat
     2701 ttttagtacc tatatcttta gcatttgatg gtgtaaaaag gtttataggt agaggtaatc
     2761 ttggaaaaag accacttgat acttattatg agatatttga cagacagaat ataaaatata
     2821 gttacaaaga aaatcagtta gatttgatta tatcaggtaa gcttaaacca gatgaattta
     2881 gagtaaaagg aaatataagt tctcagttta tcacaggttt actgtttata ttgcctacgc
     2941 tagagagtga ttcaaagata ataattacta ctgagctaga atctaaagga tatttagatt
     3001 taactctttc aacgataaaa gattttgggg tagaaattat aaacaataac tataaagaat
     3061 ttattataaa aggaaatcaa acgtataaag cgagagatta taaagtagaa ggagattatt
     3121 ctcaaggagc attttattta tcagcagatg ctataggaga agatattagc atattagacc
     3181 ttaaagaaga ttctcttcaa ggggatagtg aagtagttga aattttatct aggatgggaa
     3241 tggaaatatt aagagaaggc aacaaaataa aaggaataac aaatggttta aatggtactt
     3301 tgatagatgc atctcaatgc ccagatataa tacctgtatt atctgtagta gcatccttaa
     3361 gtatagggaa gactacaata attaatgctg gtaggcttag aattaaagaa tgtgatagac
     3421 ttcatgcaat aaatgttgag ttaagtaaac ttggagcaaa tatagaggag aaagaggata
     3481 gtttgataat cgaaggagta agcaagttaa atggtggagt agaggtgtgg agtcacaaag
     3541 accatagaat tgctatgaca cttgcaattg cttcttgtag atgtgataaa ccaataatat
     3601 taaaagattt tgagtgtgta tcaaaatctt atccacattt ttttaaagat tttaagatgt
     3661 taggaggtcg aattgatgag tggaatatgg ggaaataatt taaaagtatc aatttttgga
     3721 gaatctcatg gaaatgctat aggtataaat atagatggtc ttccttcggg gattgaactt
     3781 gaccttgata atattgataa agaaatgaag agaagagcac ctggaaaaaa ttctatatct
     3841 acttcaagaa atgaaagtga tataccagaa attctaagtg gatattttaa tggaagaact
     3901 acaggaacgc cattatgtgc aatcataaga aatagtgata ctcgttcaaa agattatgga
     3961 gagcttaaaa atttaatgag accaggacat gcagatttca caggtaatgt gagatattct
     4021 ggatttaatg actatagagg gggggggcat ttttcaggta ggataacagc tccattagta
     4081 ttttgtgggg ctatatgtaa acaaatttta tcacaaaaag gaatagagat aggagcacac
     4141 ataaaaaaaa ttaaaaatat agaagatatg agttttgatt atgtaaatat ttcaaaacaa
     4201 cagttatcaa atttgcaaac tttggaacta cctttacttg atttatcaaa ggaagaagcc
     4261 atgaaaaata cgattataga tgcaaaaaat caaggagatt cagttggtgg tataattgag
     4321 tgtgcagtag taggtattaa tgtagggctt ggaaatccat tttttgactc tgttgaatct
     4381 actttatcac atttattgtt ttctgttcct gctgtaaaag gtgtagagtt tggtttaggt
     4441 tttgaacttg cagatatgta tgggtctcaa agtaatgatg aaatgtatta tgaaggaaat
     4501 caagttaaga gcaaaacaaa taacaatgga ggaattatag gagggattac tactggaatg
     4561 ccaataattt tcaaagtggc aattaagcca actccatcaa ttagcagaca acaaaacaca
     4621 gttaatataa aagataaaaa ggacgatatt ttatatatta aaggcagaca tgacccttgt
     4681 atagttcaaa gggctatacc tgtaatagaa gcagtaacag ccattggaat ttttgatttg
     4741 atgaaaggac gataataatg gaggatttaa cgagatatag aaatgagatt gacaatatag
     4801 ataaagagct tattcaatta tttgaaaaga gaatgaatac agttcttgaa attgccagac
     4861 ataaaatgaa aaataacact actatacttc aaaaagatag agaagaaaaa gtactaagta
     4921 gagctgtaga taatttagat aataaagcat attcacaaga aacggtacag ttttttaatt
     4981 ctataatgga aattagtaga ggattacaaa aaagattgat agataatgat gatgagcaga
     5041 aaatcgagtt tagtttagat tcatcaaata agaaatttga tttgtcaaat gtaaataaat
     5101 ataagtcttt aaaagatgaa ttaaataaaa aaaatatcct agtagggttt ccaggaaaat
     5161 caggttcatt tacagaagaa gcactaaata aattcttcaa caaaaaaata tcaaaaaaac
     5221 aatttaaaga atttgaagat gttttcatag cacttaaaaa taaagaaata aactatggaa
     5281 taatccccat agaaaattca tctacaggag ctatctctga aacttatgac cttttgagaa
     5341 agtacggttt ttatatagtt ggagaagaat gtataaaaat agaccaaaat ttaataggga
     5401 taaaagacac aaagcttgaa gatataaaag aaatatattc tcatccacaa ggtataggac
     5461 aatgtagtga atttttaaaa caaaatagta catggaaatt gataccattt aataacacag
     5521 caacaagtgc agaacttgta agaaaacttc aagataaaac aaaagcagca attgcaagca
     5581 aaaaagctgc aagtatttat ggattagaaa taataagtcc ttgtataaat gatatagcaa
     5641 acaactatac aagatttgta gtaatcagta atcaaataca cattgaagaa gaatcaaaca
     5701 aaatgagtgt agtattttct gttgaacatg aagcaggaaa actttataaa gtacttggat
     5761 actttgctaa aaataatata aatatgacaa aaatagaatc aagacctatg aaaaatgcct
     5821 catggagata tttcttttat atagattttg agtgttctat atacaatgct aaagtgtacg
     5881 atttattaga attgatagaa cataatacag catattttaa atttatggga gtatataaaa
     5941 ataaaactga atagataaga taaattttta tttaatttat aattattagg aggtgtaagt
     6001 atgaattttt ttggtctagt aggtgaaaaa ctctctcaca gtgtttcacc tcaaatccac
     6061 aagagagtat tcgagatatt gaatattgaa agtgcttata aaaactttga aatttcaaaa
     6121 gaagatatat ctaagctaga tggtgctata aaattattag gaatacaagg ggtaaatgtt
     6181 actgtaccat ataaggagcg tattatgaaa tatcttgatt ttatatcacc agaggcaaaa
     6241 cgtataggag cagttaatac aattttactc agagaaaata tgttatatgg ttacaataca
     6301 gattactttg gtcttgatag tatgtttaaa atggctaata ttgatgttca aggaaaagta
     6361 gcagttatat taggaactgg aggtgcttct aaagctgctc ttacatattt tattgattct
     6421 ggtatagaaa agctttatgt ttcaactaga aaaaaggatg ataaaaaatt attaaatagt
     6481 aaagctatat taatagatta tgaagaactt aaacatataa aaggagatat aatattaaat
     6541 gctacaccag taggtatgta ccctaatgta ggaatttcac cagtgtcaaa atctattata
     6601 caaaattttg atatattaat agatttaatt tacaatcctg gagaaacaga gtttttaaga
     6661 attggtaata gtatgggtaa aaaaacttgt gatggattat atatgctggt agggcaagca
     6721 ataaaatctc aggaaatttg gcaagatact aaaatagata atagtatttt agatgttata
     6781 tataatgaac taaaattaga atttttgtaa gggagattgt tatgataaat aaaacaaaag
     6841 aaaaattgat attaataggt atgccaggta gtggaaaaac tacaattggt aaacttttgg
     6901 ccaaagaata taattgtagt ttctgtgata tggatgatta tataattcaa atatctcaaa
     6961 aaagtatagc agagttgttt tcagaaggag aagatatctt taggaactat gagactcaag
     7021 catgtagaga actttctata agtgataaaa cagtgatatc tacaggtgga ggtgtaataa
     7081 aaaaggatgt taatatggaa atactaaaag aaacaggtat aatgattttt attgatagac
     7141 ctatacaaaa gattttggaa gatataaata ttaattcaag accattatta aaaaacggaa
     7201 aagatagatt gtataatctt tataatgaga gaataaatct ttataaaaaa ttttcagata
     7261 tagagatttt aaatgataaa tcattaaaca atgctgtata taatattaca aatgcagtca
     7321 gtgaaaattt taaatttgac tttaaagaaa aataatctag ggggaagtaa aaataatgaa
     7381 tattgttatt gtaggactag gagttatagg aggctctttt gctaaagcat taaaaaaagc
     7441 tgggtatgaa aatgtatttg gagtagatgt agatttagaa actttaaaaa aagcagaaaa
     7501 agcaaaaatc ataaaaaaag gatgtactac aggtaaagag ttatttaaaa aagctgattt
     7561 aattatacta tctatatatc ctagattagt tgtagatttt ctaaataata ataaaaactt
     7621 ttttaaaaaa gggactataa ttactgatac tacaggaata aaagaaacac ttatcaatga
     7681 tgtgttgcaa attattccag atgatataga ttttatattt ggacatccaa tggcaggtag
     7741 ggaaaaaaag ggtattgatt ttgcaagtga acaagttttt aatggcgcaa attatataat
     7801 tactccaact ggcagaaaca acataaaaaa tttagagtta gttgaaaatt taattttaga
     7861 gattggattt aaaagagtaa aaaagttaac ttcacaaaaa catgatgaga ttatagcttt
     7921 tacttctcag ttacctcatg ttatggcagt tgctcttata aatagtgatg aagaaggtag
     7981 agatacgggt aaatttattg gagatagtta tagagattta actagaattg caaatatgaa
     8041 tgaagactta tggagtgaac tttttttagg aaatagagat aatttactaa aagttataga
     8101 aaactttgaa tcagaagtaa atttagtaaa agaagctata tttaacaatg ataaaaataa
     8161 attaatagag tattttaaaa aatcatcaat aagaagagaa gctttagaaa aataaatatt
     8221 aatatttaag ctatatatag atgggtataa atataagcga tagtgtattt aattgtatta
     8281 ataaatatgg attcaatata attaaatatg ctattgatat atttgtaccc ttaactattg
     8341 ttatctttta aatgataatg atatttattt aaatactctt tattaacaat ttataaaact
     8401 taattgtaaa aaatacttat taatagtaaa ataaagtgaa tcaattatat tataattgct
     8461 aaagagagtg aggtgtagct atttgaaaaa aaatagatta atagcaaaga atatatcaat
     8521 agcatttata gtactattct tttttagtgt atttacattc ttttatgtag gtaatattaa
     8581 tagagtgtta gaatatgaaa caaatgacat aataacagtt acaattgctg gatggattat
     8641 actttcattt ttatttttag gtattataat ttacatatta tattcaaaag caaatagtca
     8701 aaaaactata gaaaaagtag catatacgga ttttgtaaca ggttattcca actggagaaa
     8761 gtttgaatta gatgttacaa atttattaaa gaaaacatca caaaataata aatatgcaat
     8821 ggtcatattc gatattgata aatttaaagc aattaatgat atatatggcc ataaaaaggg
     8881 aaatttaata ttaaaagata ttgcagatac tttaaatgaa ttgactaata tcaatgaaac
     8941 ttttgctcgt gtaagtgctg ataatttcaa tatattatta acatataaca aaaaagagga
     9001 tataataaat attatcaaaa agattatggc aaataatgaa cttgtaaact tatcttttgg
     9061 aatttatgaa atcaaagata aggatttatc tgtaagtgta tatagtgata gagcttcact
     9121 tgcaaaatca tcaataaaaa ataatagtga tgtaaatttt gcatttttta atgataaatt
     9181 aagagagaaa ttattacttg aagataagat agaaaaagaa atggaatatg ctcttgaaag
     9241 tgggcaattc gtaatgtatt tacaaccaaa atacaatatt aaattagata aattttgtgg
     9301 aagtgaagcc ctagtcagat ggcaatatac agaaaaagag gtcatatatc caggtgattt
     9361 tattcctata tttgaaaaaa atggatttat tagaaagatt gatatgtaca tattagaaca
     9421 agcttgtaaa gaaatccggt ctttatttga taaaggaatt tcacctttac ctatatctgt
     9481 aaacttctca agagttgatt tttttaaaaa agattttatt gaaaacatag taaatatatg
     9541 tgatagatat aaaataccat atagtttaat agaaattgaa attactgaat ctagtatgtt
     9601 tggagatact gatactctat ttaatgttag tagaaactta caggatattg gctttatagt
     9661 tgcaatggat gattttggct cgggatattc ttctgtaaat atgcttaaaa atataccatt
     9721 aaatgtaata aaattagata gaggattttt tgttgatgat aaagatgttg ataagagtca
     9781 aattgttata aaaagcatag tttctctcat taagcaatta ggaataaggg ttgttgctga
     9841 aggaattgaa acaagaagtc aaatagaaat gttaaaaaaa gcaaattgtg atattgttca
     9901 aggatattat ttttcaaaac cattaccaat taaagaattt gaaaaattag tctataaaat
     9961 atagtcagcg agtcttaaat gggaggtggt gattaagtga taagagcaaa atcgttaaaa
    10021 aatgtactga gtattgtttt aatattttta tttacaataa tttctttttc tgattttgca
    10081 tttaaaacta atgtccagga agaagaaaat agtaaaatta atttaagaga aatatcccta
    10141 caaagttcca atgctataaa aataaaatta gatagttctc tatcctttat taaaagtgtg
    10201 gcaaatatgg tttcttattt taatactata gataactcaa aaatagttcc attattagct
    10261 aatatatcaa aagaatctga ctttcaaaga atctctattg tatctcctga tggaataagt
    10321 cataccagtg atggcaaaat tatcaatgtg tcaaagtatc agtattttaa gaaagctatt
    10381 aaaggagaaa gtaatatatc tgaagttgtg aaatctccaa ttgatggaaa agatactttt
    10441 gttatagcta ctcctgtatt taataagaat aacaaaataa ttggtgtttt atacggttct
    10501 tactacaatg ataaattagc tgaatttata gatataacaa gttttaatag tgaagggtac
    10561 atagatatat ttgaatctag tggtcaattt gttttaaaat cttcacatac aaacaaactg
    10621 attaagtcaa gtagtaacat atatgatata tttaaaaaag cttcttttga tgatggttat
    10681 agctataaaa aaatatatga agatacaaaa aataacaaga gtggctttac ctcatatgaa
    10741 tacaaaggag aaaatcgtta tgctacttat acacccattg gtgtaaatga ctggtatgta
    10801 atttctgtga taccacaata tactgtaaga agaaacattg ataaaataaa tcaattagca
    10861 ttttctctta ttttctaggt attcttattg tttattatgc ttataatata tattatttat
    10921 aaaaacaata aatctcagaa agatatacta aaagctaaca aagaaatctc atctattaca
    10981 aatagcattc ctggaggagt acaaaagtgt accctgggtg aaacatgcaa tattaatttt
    11041 ttaagtgatg gatttataga gttgtttggt tatacaagag aagaaataaa aacattgttt
    11101 aataatgatt tttatagttt aatacataaa gatgatgttg aaagaataaa aaaagaaatt
    11161 tttaaacaac tttacaaaaa taaaactatt gaattagaat ataggattat aacaaaaaat
    11221 ggtaatacag tatgggtttt agataaaggg gagcttataa cagaacctga tggaaaagaa
    11281 tatttttgtt gtgtaactgt agatattact agaacaaaac aattagaaca agagatggaa
    11341 caaaaaaatc tagaacttga aatgatatca gaaaatgttt taggtggtat aattataaca
    11401 gaatttaatg atgattttac tataaaatac gctaactctg gttatctttc aatgattgga
    11461 tatacacgtg aacaattaga aaatgaactt aaatacaaag cttgtaatct tgttttttat
    11521 gaggataaaa agaaggctct tgaaagtata caacatcaat taaacacaaa aggaatagct
    11581 gtatatcaac atagaataag aagaagaaat ggtaatatta tttgggtttc tataaaagga
    11641 aggcttattg ttgatgatga tggtattgaa aaagatattt ggattctaaa tgatataaca
    11701 gatttaaaaa atgcagaaca aaaacttaaa ataaatgaag aaagatttag aattgcatta
    11761 aagcaaacat caaacactat atttgagtat gatataaaaa caaagtcact tttaaatact
    11821 agtaaagagc ttgataaata taatatttat aaaattacta aaaacgttcc atattctcta
    11881 gtagaatcag gagttattca taaagatttt agtgatgatt ttataaatat gtatgaaaat
    11941 gtaataaatg gagcaaaaaa agcaagttgt gtagttaaga tgaaacttcc agatgaaaat
    12001 tatatatgga ataaagttac cttgactact atttttgatg aagatggtaa tcctgtaaga
    12061 gcaattggta tacaggaaga tataagcaaa caaaaggaag cagagataag atacaatcaa
    12121 gaaggtaagt atcattcagc tatattgtcg gaagcaatga taacctatga aataaatgta
    12181 tcaaaggata cttttatcaa aggaaatgaa agttggcaaa aaccatttgg aattaagcct
    12241 gacaataact attctcaaat ggtaaaaact atagcaaata gtattgtaca taaagacaat
    12301 aaagaaacat ttttaaatac atttttatgt gaaaacttat tggatgcata taaaaataat
    12361 gtgactgaaa taaaattaga atatcgaaga atgaatcaag aaaataaaat gatttgggtc
    12421 atttgtacaa tgcacttaat aaaagattct attacgaacg atataaaagc acttgcttat
    12481 gttaaagata ttgacaagca aaaaaatgaa gaaattagat taaaattaaa agctgaaaga
    12541 gatactctta caggattata taataaaggt actacagaaa aattgattaa tgatttcttg
    12601 aactctgagg aagcgagctt aggaatgcat gcatttttaa ttgttgatat agataatttt
    12661 aaatctgtga acgataattt aggacatgta tttggagata aagtattatt tgaagtttct
    12721 aaaaacctaa aacctatatt tagaaaagat gatatagtag gtagaattgg tggagatgaa
    12781 tttatagtat tcttaaaaaa tattcagtct tttgagaata tcaaaaagaa agcagaaaaa
    12841 atatgcgata tatttagaaa ttcttataca gaaaataaaa agacatataa aatatctgga
    12901 acagttggaa tttcatttgt accagaacat ggaactgtat ttgaagaact ttataaaaaa
    12961 gcagacatag cactttatta tgcaaagagc aaaggaaaag ataagtatgc actttataat
    13021 gattgtatct accaaaatga acttgaaaat aatataagtg ataataatat aaaattagca
    13081 gataaatgtg agaaatccaa accagtactt aaagaaattc ttgattctat tgataattat
    13141 atctatatag tgaatccaaa tacttatgaa cttctttttg tcaataataa ggtgtctcgt
    13201 ttggatttag agataaaatt aggagaaacc tgttataaat cactattaaa atctgataga
    13261 ccatgtaata attgccctct taacggatta aaagaaaata agcattcaaa attcaaaata
    13321 gagacaaata catttggtaa aaatatcagt atagcaagtt ggattaattg catagatgga
    13381 gaatacaact gtcttataga gtgtatagat tcttctttat acaaaaataa ataaaaaagg
    13441 actatgaaga tatgttaaat agtataactt tgatacttgg tataatattt acaatttact
    13501 atttttatct caaaatagtt tttggaggaa tttcatttag tgagattttc ttagttgtag
    13561 gaattgtact aattatctat caaatattta agaaaaaaat taaagaaaag aaggtttttt
    13621 atagggtgtt aaaaataata atcagcattt ttttaatagt atttgtagtt actgagagtc
    13681 taattatttt ttatcctaaa aatagtttag aatcaaaaag tgattatctc ttaattttag
    13741 gagcttctgt aaaaaagact acaccaagta caaccctgaa gggtaggtta gacactgcat
    13801 tgaaatacct aaaaattaat gataattgtt atgtggtagt atctggtggt aaaggaagtg
    13861 gagaaaaaat aactgaggca aaagctatga aagattatct tataaaaaat ggtatagata
    13921 aaaataagat aatagaagag gataaatcca cgaatactta tgagaatttt aaatattcaa
    13981 aattgaagat agaagaacat agtcaaagaa aactaagcga tttaaaagta aaaatagtaa
    14041 caacagattt tcatgttctc agaagtaaaa tattagcata tagaaatgga tataaaaata
    14101 cgtcttttta tgttagtaaa tcaaaacttt catttgtacc aacttactat acaagagaat
    14161 tttttgcatt atggaaaaca atcatttttg atagataata aataaaaaga ctagatataa
    14221 actcatatct agtcttttct attcccttaa ttctaatcca ttttccaact ttacttgttg
    14281 tagaaatttc aacattatta tattgattat tcaaaattat atataaaatt cagaaaaaat
    14341 tttagtttac ataataaaaa taaagtcaac taaaattatt gattttagat taaagtacca
    14401 gattaagcat ttaagtagat tttaagcttg atttgattag aaaggtacaa actatcaccg
    14461 aaaaaattaa acccatttaa aaagcaatat aaaagcttgt tatttttgag aaaaatagac
    14521 cctcta
//
LOCUS       DAEMHW010000093        14446 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEMHW010000093 DAEMHW010000000
VERSION     DAEMHW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.093"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..265
                     /locus_tag="KPK67_002287"
     CDS             <1..265
                     /locus_tag="KPK67_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBG2459245.1"
                     /translation="DKKIRETYLLSKEGKSYEFIKRLLRDFKYVYYYGIENCFIEEYF
                     KYTDKSIEVNPLRLKDKVKVENNFDADLEDNRINYINSIGMII"
     gene            266..709
                     /locus_tag="KPK67_002288"
     CDS             266..709
                     /locus_tag="KPK67_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459246.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            724..1308
                     /locus_tag="KPK67_002289"
     CDS             724..1308
                     /locus_tag="KPK67_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459247.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            1561..2598
                     /locus_tag="KPK67_002290"
     CDS             1561..2598
                     /locus_tag="KPK67_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG2459248.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            2679..3044
                     /locus_tag="KPK67_002291"
     CDS             2679..3044
                     /locus_tag="KPK67_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459249.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKDIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            3049..3366
                     /locus_tag="KPK67_002292"
     CDS             3049..3366
                     /locus_tag="KPK67_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459250.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYICELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            3414..5018
                     /locus_tag="KPK67_002293"
     CDS             3414..5018
                     /locus_tag="KPK67_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459251.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            5142..6770
                     /locus_tag="KPK67_002294"
     CDS             5142..6770
                     /locus_tag="KPK67_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG2459252.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            7035..8384
                     /locus_tag="KPK67_002295"
     CDS             7035..8384
                     /locus_tag="KPK67_002295"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBG2459253.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGSNDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            8540..9619
                     /locus_tag="KPK67_002296"
     CDS             8540..9619
                     /locus_tag="KPK67_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG2459254.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            9964..10872
                     /locus_tag="KPK67_002297"
     CDS             9964..10872
                     /locus_tag="KPK67_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG2459255.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            10877..13246
                     /locus_tag="KPK67_002298"
     CDS             10877..13246
                     /locus_tag="KPK67_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBG2459256.1"
                     /translation="MARGTFERTKKLREESVNAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            13571..14374
                     /gene="proC"
                     /locus_tag="KPK67_002299"
     CDS             13571..14374
                     /gene="proC"
                     /locus_tag="KPK67_002299"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG2459257.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT         5935 a         1476 c         2652 g         4383 t
ORIGIN      
        1 agataaaaaa ataagagaaa catacttgct atcaaaagag ggaaaatcat atgagttcat
       61 aaaaagatta ttaagggatt ttaagtatgt ttattactat ggtatagaaa attgttttat
      121 tgaagaatac tttaaatata cagataaatc tattgaagta aatccattaa gattaaaaga
      181 taaagtaaaa gtagaaaata attttgatgc agatttagaa gacaatagga taaattatat
      241 aaatagtata ggaatgatta tttaagtgaa agtggataaa acaataaatt ttcttaatgg
      301 atttaaaaaa actaaaccat gtaataaaat ctataaaata ataatagtct taattttaac
      361 gtgtatttta tttaaatcat ttatgtatgt gaaatctata aatgaacttg aagattatat
      421 aagtcaaaat aagctagtgg ataaatcaaa gaacacttta ccaacaacag ataaaactag
      481 agagaaattt tctaaaataa aacttaatga aataaaaaaa atatatgaaa caattggaat
      541 aaatagtatt actaaaatat caatagataa aaataagcta gagatggaag gtgtgtgtag
      601 tgatttagat gttttaaaca atttaaggaa aataaagtac ataaaatatt attacataga
      661 cagtattgta agagatggag aatattatag atttagaata aaaagttaga tgggtgatta
      721 tttttgagat taaataatag agaaaaaaat ctacttaaaa tattgttaat attagttatt
      781 atattggcaa gtgatatagc agtaagttat ttggaagaaa acctaactag tttgaagaat
      841 gaaaagcaaa atataataaa taaaataaat aatcaagata attatcaaat aaaggataat
      901 aagcatatag atgataaata tatagaaagt gttgaattaa ataaaaatga agaattgaaa
      961 aataatttta ataattcatt tttagataaa aaaacaaata aagatatagt gttattagta
     1021 gaaaatgaac tgagagatat tgtgaatgta gaatatatta atcaagaaag tagtttagat
     1081 aatcacaata aaaaacaagt taatttagaa ataaaagtta atggagaact tgcaaatgtg
     1141 ttgaaacttg aaggtgtaat gaagaagtta agactaaata ataaacttga aaagatagaa
     1201 attaacaaat taaaagtcaa taattttgat gttcaaaata ttccaaatga atataaacaa
     1261 gaaaaagaca atattgttga atgtaaaatg acagttagag tcatataaca aaaaagaata
     1321 gataaagtaa aaaactatta tcaattaata aaattttata gaggaattag ttttacgata
     1381 attcatttct acaagaggga ttgttgatga ttttatcgaa atcctaatta aagttaatat
     1441 attgtaccaa aatataagtg aatttaatag aatatttact tatactatag attgattaat
     1501 aaaaaaatgg tataataaca aattgtactt taatttaata ataagggagt ggaagtcata
     1561 ttgttaggga gaaatgagct atgcccttgt ggaagtggca aaaaatacaa gaaatgttgt
     1621 ttaaataaag atattgtttc agaaagagca tgcagaaaaa ctgaactttc acaaaaacaa
     1681 tatacagatt tatattcaaa gttatatgat tattcaagac aggataagtt taaagaagaa
     1741 tgtgaaaaag ctaaagaaat gttttatata atgcaaaatg atagtgtaaa tgaaaaattt
     1801 gaaagatttt ttaatactta tttaatacaa gaccacataa tggaaaataa aaaggttatg
     1861 acagttggtt ttcttgaaga aaatggtgca aacctaagtc aatcagaagt atcaatatta
     1921 aaaagtatgt ttgaatcata cgtaagtatt tatgaggtaa aagaagtttc tgctggaaaa
     1981 atagtattaa aggactgttt aagtggaaat gaactttgta cagaagatgt aaaacttctg
     2041 agaagtttta aaatcggaag ttgtatgata gctagaatag ttgatattga aggaactaac
     2101 atattgatag atataactat aagtatatca aatgaggtta aagatataat tttaaaggat
     2161 attatgaatc tattcaatca atatcaagat gtatacaaag atatgaagtc gttcttaatt
     2221 tatcatacac atatactata caagtatata caacagcttt tagacccaag tatagctgaa
     2281 tatttaaaga gagaaagaga taaaaacgat acaaaagtta attcagaaaa tgaattagct
     2341 gaagaagaat gtaaagtttt agatactctt aaagaaaatg tagaaaaaga aaatatagat
     2401 gcatgtatag agttttggaa tgaatataag aaaaatcatg aaaatataaa aggagctgaa
     2461 aatggttggg cagctgctgt tgaatactat gttaaaaaag aagcaggaca acctataact
     2521 caagttcaaa tatcaaagaa atacgatata agctctagta cactaggaaa gagatacaag
     2581 gatttaaaaa tatcttaatt aattataaaa agctttaagt catagataaa gaatctatag
     2641 tataatatat gtataatgtt tagaaagagg tattaataat gagtcttagt ttaggaaatg
     2701 caattgctgt atcaacgaac tttataaaat ctaacccagc tgtacctgtg tacaatggag
     2761 aagaaatata tgcagttatt tatcatatag aaataggata taataatggt gtatcacctg
     2821 ataccaaaca atatataata atagactata aagagtttga tttatctatg gataaaaaaa
     2881 atgaactaat aaatattgct aaagagtact gtaaatctaa agatattgta gaaaacgaaa
     2941 accttgaaat aaacctttta gacaatgaag aagcagagaa cttcttagaa aaggtgttta
     3001 ataatatgaa ggttataaga ggtcttataa ctaaaaaagg ataaataaat ggactatata
     3061 ggaatagaaa atataacacc ttatgaaaat acatatgaat ttagtgtata tgaatatgat
     3121 gatgaaatca ccttaggtag tgaaaagtta tatatatgtg aattaagggt tgtattgatt
     3181 aaagttaatt ctctgtatgt tgaaagattg cataaatcag ttgaagcaat ggtcttagta
     3241 aaaaacttga aaaaagattt agataaaaca cttgttgtaa acaaaataaa gaattttgtg
     3301 ctagatgaga tttgggtaga aaatctagta aaagagaata tagaagttat atttgtagaa
     3361 agctaggaat aaccctagct tttttatatt acaatctaag agaggaaaac aatatgttaa
     3421 gagtatctaa tgtaaaactt ggaattgata aagatatatc tttattaaaa gatgttatac
     3481 ttaagaagct aaaaataaaa gaaaaaaact tagtcaaata tagtatatat aaagaatcta
     3541 tagatgctag aaaaaaagga aaaatggaat ttgtttattc tgtagatgta gaagttaaag
     3601 atgaaaataa aatacttaac tcaaaaataa aagatgtaac aaaaataaag gaaatcagat
     3661 acgtaaatgt acctgtgggt gataaaaaat taaagaataa tcctttggta attggtagtg
     3721 gaccagctgg tttgtttgca ggtctgcttt tagctcaaat gggttatgaa ccaatcatac
     3781 ttgaaagagg tcttgatgta gacagtagaa ccaaagatat aagtgatttt tggacaacta
     3841 gaaaattaaa aaataattca aacgttcaat ttggagaagg tggagcagga actttttcgg
     3901 atggtaaact tacaactaga ataaaagata tacgatgtag aaaagtatta gaagagttag
     3961 ttcagtttgg agcaccagat gaaatattat actcacataa gccacatgtt ggaacagata
     4021 tattaaaaaa tgtagttaaa aatataagaa atgaaataat aagtttaggt ggtcaagtta
     4081 gatttaattc aaaagtaaca gatatagttg tatcaaatga ttctatacaa tctgtaataa
     4141 taaatgatac tgaaaaaata gatacagata caattatatt agctgttgga catagtgcaa
     4201 gagatactta tgaaatgttg tattcaagag gtataaaaat agttcaaaaa ccttttgcaa
     4261 taggagctag agttgaacat ccacaagatt taataaataa agcacagtac aaggattttt
     4321 ataatcatcc taggttagga gctgcagatt atagacttat agagcatgtt tcaaatggaa
     4381 gaacagcata tactttttgt atgtgtccag gaggtagtgt catagcatct gcatcaaatg
     4441 attttgaaat agttacaaat ggtatgagtg aacatgcaag agataaaata aatgcaaata
     4501 gtgcattttt agtaaatgta acgccagaag attttaagag tgataatcca ctagcaggtg
     4561 ttcatttcca gcagaagtat gaaaaattag cttttgaatt aggtggaaaa aattataatg
     4621 caccagttca gcttgttgga gattttataa acaataagac ctctttgaat ttaggaagtg
     4681 ttgagccatc atatagacca ggatatacac ttacaaattt aagtgaatgt ttgcctagct
     4741 tcgtaacaga gactatgaga gaagcgttta tatctcttga taaaaaattg aatgggtttg
     4801 caatgtatga tgctgttttg acgggagtag aaactaggtc atcatcacca ataagaatag
     4861 taagagatga aaatacttta gaatcagtga gtattaagaa tctatatcca tgtggtgaag
     4921 gtgctggata tgcaggtggt atagttacag cagcagttga tggtataaaa tgtgcagaaa
     4981 aaataataca aaaatacacc aataatacaa aaatttaaca taaacttaat gattttttaa
     5041 tatttaacat gaatttaaca ttatcactcc tataatttga taaaatacaa taggttataa
     5101 aatttttaga tacagtaatc tctttacaag gaggaaataa tttgaacatt ataataagtc
     5161 taatgggtgg attaggtctg ttcttatatg gtatgaacct tatgggagaa ggtcttcaaa
     5221 aatctgctgg gacaaagcta aaaaagataa taaaactact tactagtaac ctatttatgg
     5281 gagttttggt aggtacagga gttacagcag taatacaaag ttcaagtgct actactgtaa
     5341 tggttgttgg tttcgtaaat gcaggaataa tgactttaaa acaggcaata ggagttataa
     5401 tgggtgctaa tatagggact actgttacag cccaattggt atctttcgac ttaactggta
     5461 tggccccagt agcattaggt gtaggtatta tactttattt atttggaaat aagcctagaa
     5521 taaaaaatat agccgaaata cttataggtt ttggtatatt atttacaggt atggatttta
     5581 tgaagatggc agtggagcca ctaagagact atcaaggatt tactgactta cttgttacat
     5641 ttggtagata tccactactt ggattattat tgggatttgg gattacagca ataatacaaa
     5701 gttcaagtgc atctatgggt atgttggttg cactggcagc agaaggtcta gtgccattat
     5761 cagcagcact gccaattctt tatggccaaa atattggtac atgtgtaact tcactgttat
     5821 caagtattgg tgctaacaaa aatgcacgaa gagcagctat gatgcatcta atcttcaatg
     5881 ttttaggaac ggtgatattc ttaatattct tgaataaacc agtagtaagc atggttacaa
     5941 gttgggaccc aagcaatgtt gcaagacaaa tagccaatac acatacattg tttaatataa
     6001 tatctgtatt gatactttta ccatttacaa atcttattat aaaactagct ataaagcttg
     6061 ttcctgatag agcaggtgat atagatgaag atgaaactaa aactattaaa tatatagatg
     6121 atagaatgat agaaactcct tctatagctc ttgcaaatac gataaaagaa gcacttcgta
     6181 tgggtgagaa ggcgaaggaa agcctaaatg catctatgga agctttagta gaacattcaa
     6241 ctgaaaaaat agataagaca tataggcgag agagattaat caatgattta cagaaagcta
     6301 ttttaaatta cttacttaaa ctttcaaaag cacctttaga tgatgattca agagaagttg
     6361 tggatacttt gtttaataca gtcaatgata tagaaagaat tggtgaccac gctaaaaata
     6421 tagctgaatt atcgcaagtt gcaatagatt caaatatttc tttctcagaa gaaggacaat
     6481 ctgaacttga tgttatgtat aatagagttg tttcagcata tacttatgca ttagaatcaa
     6541 tgagaactga taatgttgat ttagcttgta aggttataaa aattgaagaa caagttgata
     6601 taatggagaa atcttgtaga gctaatcaca tgtatagatt gaacaataat ctatgtagta
     6661 tagaaaatgg agttatttat ttagacgtaa taagtaattt agagagaata tctgaccatg
     6721 ctgtcaatat agcacaacaa gttatagcaa agagattagg aaatgattaa aaataattaa
     6781 tttaaaataa tataaaattt aaaatagagg ataaataaaa gagaaacttt aaatgatacg
     6841 tttagagttt ctttttttgt aaatttattt gactaaaatt tttaaattta tacatagtta
     6901 tagtatttag aagaatcgat ttcctgataa taaatgatga gatatgtatg attttgtggc
     6961 aaacttttaa aaaaaacacc tatattttgt gttataatct ctttaaaaag aatatgctga
     7021 aaagagggat ttaaatggga aaaataaatt ttgattatag taaagctaca gagttttttt
     7081 gtcaaaacga aatagatgtc atgcaaccat atgtagatgt tgcgcacgac atgttacata
     7141 ataaaactgg tttaggaaat acttttttag gatggataga ccttcctaaa aattatgata
     7201 aagaagagtt tgacagaata aaaaaatctg ctgataagat aaaatcagac tcagatgttc
     7261 ttttggtaat aggtatagga ggctcttatt taggttcgag agctgctata gatatggtaa
     7321 gtcattcttt taggaatgga cttaaaaaag agcaaaggaa ggctccagaa gtatattttg
     7381 tagggcataa tataagctca acatatatta tggatttatt agatataatt gaaggaaagg
     7441 atatttctgt aaatgtaata tctaaatcag gaactactac agaaccagct ctggctttta
     7501 gaatatttaa agattactta gagaaaaagt atggtaagga agaagcaaga aagagaattt
     7561 atgcaacaac agatgcaagt aaaggtgcac ttagacagtt agctacagaa gaaggatatg
     7621 agacttttgt aataccagat gatgtaggtg gtcgtttttc tgtattgaca gcagttgggt
     7681 tacttcctat tgctgctgct ggacttgata ttgatgctat gatgaaaggc tcaaatgatg
     7741 ctagagaagc atttcaaaat cctgacttaa aaagtaatga tagttataga tatgctgttg
     7801 ctagaacaat tttacataga aaaggaaaag atgtagagtt attagtgaac tatgaacctc
     7861 aactacatta tgtaagtgag tggtggaaac agctttatgg agaaagtgaa ggaaaagaaa
     7921 ataaaggttt attccctgct tctgtagatt tctcaacaga tttacattct atgggtcaat
     7981 acatacaaga tggaaaaagg cttttatttg agacagtact taatgttgaa aactgtaaga
     8041 gaaatataac tataagttca gaggaagtag acttagatgg tttaaactat ttagcaggta
     8101 agactgtaga ttttgtaaat cataaagctt ttgaaggaac tctattggct catacagatg
     8161 gaaaagtacc taatttagta ataaatatac ctcaactaga tgagtataat tttggatatt
     8221 tagtttattt ctttgagaag gcttgtggaa taagtggata tttattggga gttaatccat
     8281 ttgaccaacc aggagtagaa gcttataaga aaaatatgtt tgctttacta ggcaaaccag
     8341 gttatgaaaa agaaaaggaa gagctagaaa aaagacttaa ataattttaa atagttaagg
     8401 tttaattaag attaaaataa tataatgaaa tcatcaaaaa ggagtttact caaaatagat
     8461 gatttcattt tttttatgaa aaattgggaa aaatatattt tgataattta tattatatta
     8521 agatagggag tggtatgaga tgtcaagaag aaagaaaggg ataagtttag taatattagt
     8581 tgctataata agttcaatac ttagtagttt tctaacaata attttagtta aggacaattt
     8641 agtaagtaaa tctacaggaa gttctacgcc gatagttgta aatgatgatg gtaagagtca
     8701 aaatatttat caagcagttg cagaaaaagc tacgccatct gttgtaggta ttacaacaac
     8761 tagtgttgat actagtaata tgtttgcaat accaactgaa acacaaggtg taggaactgg
     8821 aatcatagtt gattcaaatg gatatatact tactaattct catgttatat cagatggtca
     8881 agctacaagt gtaaatgtat tattcaatga tggttcaaca acttcaggta aagtagtatg
     8941 gtttgaccaa caacttgatt tagctatagt taaggttgat aaaactggac taacaccagc
     9001 agaatttgct gatagtgaca aagtaaaagt tggagatata tcaatagcaa taggtaatcc
     9061 tcttggatta gattttcaaa aaacagttac tcaagggata ataagtggat tagatagaac
     9121 tatacaaaca gaaaaaacta atatgactgg tttattacaa actgatgcaa gtataaatgc
     9181 aggaaatagt ggaggaccat tgttaaatca aaaaggtcaa gtaattggta taaacacagc
     9241 aaaagcttct caagcagaag gattaggatt tgcaatacca atcaatacag ctaagagtat
     9301 agttgaagaa gtaatcaaaa atggaaaata tgaaaaagta actttaggca taaaaggtac
     9361 agatgtatca aattatgaag cagcaacagg tacaaaatta agtactgata aaggtgttta
     9421 tgttgcagaa gtaatatctg gttcatcagc agaaaaagct ggagtaaagg taggggatat
     9481 tataactaaa gtaggagata cagatataac aggtatgaat gatttaaata aaaagttgta
     9541 tactttctca aaaggtgcta gtacaaaaat aacagtaaat agaggcggaa aagcagtaac
     9601 aataaatgtg aatttctaaa tatgaccaga aagatagaga ctacatggta aagtgtagtc
     9661 tctattttta tttaattttt atagtatata catctaaatt aattaaaatg atgtattgtt
     9721 tatagtttat tgctatattt tcttataaaa ataaatttaa taaatattta aatttaattt
     9781 acaaaaacaa tataaatatc ataataaaat aaaatttttt aatattacta aaaatatata
     9841 gacaaaataa taacaaatat agtattatgt aattgtagaa atgcttttca taggtatatt
     9901 ctatgaagat gaattgcata aaaatgaaaa aatggaatgg atattattta ggggtgaaaa
     9961 atcatgaatc cattagttat aaacttacaa aaatgtagca tacatgatgg accagggata
    10021 agaagtacag tattttttaa agggtgtcca cttgaatgtg tttggtgtca taatccagag
    10081 agtcaaacat atacaaaaca agttttgtat aatgaagaaa gatgttcaaa atgtgaagca
    10141 tgtataaata tatgccctca taaggcaatt tacaaaggtg aaacaaaaat atgcttagac
    10201 caagataagt gtgagttttg tgaaacttgc ttagactatt gtgtaaacaa tgcaagagag
    10261 attgttggtc aagagtattc tgtaagagat ttggttaaag agatagagaa ggatagaatt
    10321 ttctatgaag aatcaggtgg tggagtaact ctatcaggtg gagaagtaat ggctcaagat
    10381 atggacttca tttgtggagt aataaacatg tgcaaatcaa agggaatcca tgtagcaatt
    10441 gatacatgtg gctatgcaaa gtcagaaaat tatgaaagag ttgctaaatg tgcagatttg
    10501 tttttatatg atattaagct tattgatgaa gataagcaca taaaatttac aggaaaatca
    10561 aatgacttaa tactgaaaaa tgttaagatt ttaagtgaat taggggctaa cataaatata
    10621 agaataccat taattgtagg agtaaatgta gatgatgaaa atttagaggt taaaaaaatg
    10681 atagagtttt taaagccttt gaatatacaa gcagtaagtc ttcttccata tcataatatt
    10741 ggtaaacaca agtatgataa aatttataaa aagtatgaag gagaagaact acaaagacca
    10801 tcagaagaaa aattagagga aataaaaaga ctgtttgaag caagtaattt caatactaag
    10861 attggagggt aataaaatgg caagaggaac ttttgagaga actaaaaaat taagagaaga
    10921 aagtgttaat gcagaaccac acatatctat tgaaagagca gtacttatga cagaagctta
    10981 taaaaaatat gagggaagtg tagagattcc tgtacttcgt gctttatcat ttaagcatta
    11041 tattgagaat agaactctta gcataaatga tggagaatta atagtaggtg aaaaaggtga
    11101 ttctcctaat ggagcaccaa catatccaga aatttgttgt catacaatgg aagatttaga
    11161 agtaatgcat aatagagata taataaattt tagcgtatca gaagaagcta gaaaaataca
    11221 taaagaagaa ataattcctt tttggaaaaa gagacaaaca agagataaga taataaatgc
    11281 aatgacacct gagtggttag cagcatatga agcaggtatg tttacagagt ttatggaaca
    11341 aagagcccca ggacatacag tttgtggaga tacaatatat aaaaaaggat ttttagattt
    11401 aaagaaagac atagaagcaa gattaaaaga gctagatttt cttaatgatt tagatgcata
    11461 taataaaaaa gcagatttag aagctatggc aatagcttgt gatgcaatgg ttatattagg
    11521 taaaagatat gctgagaaag caagacaaat ggcagaagaa gaaacagatg aagctaagaa
    11581 aaaagattta ttactaattg ctgaaacttg tgatgttgta ccagctcaca aaccagaaac
    11641 ttatcatcaa gctatacaaa tgtattggtt tgttcatata ggggttacta ctgaacttaa
    11701 tatatgggat gcttttactc caggaagact tgaccaacat ctaaatcctt tctatgaaag
    11761 agatgtagaa aatggaatac tagatagaga tagagcacaa gaattacttg aatgtttatg
    11821 ggttaaattt aacaatcagc cagcaccacc aaaagttggt ataacattaa aagaaagtag
    11881 cacatataca gattttgcaa atataaacac tggtggaata aatccagatg gtcaagatgg
    11941 tgttaatgaa gttagttata taatacttga tgttatggat gaaatgaaat tgatacaacc
    12001 tagttcaaat gttcaaataa gtaaaaagac tcctcaaaaa ttcttaaaga gagcttgtga
    12061 aatatcaaga aaaggatggg gtcaaccagc attttataat actgaagcaa tagttcaaga
    12121 gcttatggaa gctggaaaaa ctatagaaga tgcaagactt ggtggaacta gtggttgtgt
    12181 tgaaactgga tgttttggta aagaagcata tgttctaact ggatatatga acattccaaa
    12241 aatacttgaa ttaacattaa ataatggata tgacccaatt agtaaaaaac aaataggtat
    12301 tgaaactgga gaccctagaa atttccaatc ttatgaggaa ctatttgaag catttaaaaa
    12361 gcaattacat tatatgattg acataaaaat agaaggaaat gctgtgatag aaaatatatg
    12421 tgcaaaacac atgccttgtc cacttatgtc tacaatagtt gatgattgta tagaaaaagg
    12481 caaggattac caaagaggtg gagcaagata caacactaga tatatacaag gtgttggtat
    12541 aggaactata acagatagtt taacagcaat aaaatataat gtatttgata agaaaaaatt
    12601 cgatatggat acattgctta aagcacttga tgctaatttt gaaggatatg aagctatact
    12661 taatttagtt tcaaataaga ctcctaaata tggaaatgat gatgattatg ctgatgaaat
    12721 aatgcaagag atattcaatg catattacaa tgaggtaact ggtagaccaa ctgtttgtgg
    12781 tggagaatac agagtagata tgttaccaac aacttgtcat atatactttg gagaaattat
    12841 gggagcaagt ccaaatggta gactttgtgc aaaaccagtt tcagagggaa tttcacctga
    12901 aaaaggtgga gatactaatg gacctacagc agttataaaa tcttgtgcta agatggacca
    12961 cataaaaact ggtggaacat tattaaatca aagatttgca ccatcagtag ttcaaggaga
    13021 aaaagggtta gataatatgg ctaatctagt aagagcatac tttaatatgg atggtcatca
    13081 tatacaattt aatgtatttg ataaaaatgt actattagag gctcaaaaga atcctcagga
    13141 ttataaagac ttaatagtta gagttgcagg atatagtgac catttcaata atttaagtag
    13201 aacactacaa gatgagataa taggaagaac tgagcaaaca ttctaggttt gcttgtaaat
    13261 aaaacttctt actatataaa atcgtaactg atataataaa gtataaatgg tttgattcca
    13321 tttttaaaat tttagattaa ttatgagcta actttctaaa taggaagtta gctcgttaag
    13381 tttaatgaaa aaatcgttaa atttatattt agtaaatcat acacatatta gtaagtaata
    13441 catacaaaag aataatttat tatagttata ataaggttat attttataaa aataagataa
    13501 ttttaagcag ttaaaataaa gccaatttag ctactttaaa ataaaactaa tagaaaaaag
    13561 gagatttgat atgaaaactt taggatttat tggttcaggg aatatgggaa gtgcaatgat
    13621 aggtggcata gtacagtcag gattagtaaa ggcagaagat ataactgtat cagatttaag
    13681 ccaagttgcc ttagataaag taaaagaaga atttggagta aatactacaa ctgattcaaa
    13741 ggtagctgtt acaaattctg atgtggtaat agtagcactt aaacctaata tatgcgaaaa
    13801 ggctttaact ccacttaaag aattaataga tgcaaataaa ataatagtat ctatagcagc
    13861 aggtaaaact atagaaagtc tggaaagttt tataggttca gataaaaaaa tagtaagagt
    13921 aatgcctaat actccagccc ttgttggcga aggaatgtca gctctttgta aaaatagtaa
    13981 tgtaacagat gaagagttaa atatgattaa ggctctgttt gaatcttttg gtgaggcaga
    14041 aatagtatca gaatatttaa tggatacagt tacaggtatt agtggttctt ctcctgcata
    14101 tgtatttatg tttatagaag ctatggcaga tgcagcagta ttagctggaa tgccaagaca
    14161 acaagcttat aaatttgcat ctcaagcagt aatggggtca gctaagatgg ttcttgaaac
    14221 tggaaaacat ccaggtgaat taaaagatat ggtttgttct ccagcgggaa ctactataga
    14281 agctgttaag gtattagaag aagaaggctt tagagcagca gttataaaat ctattgttgc
    14341 ttgtatagat aagtctaaag atatgagcaa ataacagatg taattaagtt tactatatgg
    14401 tttcttgaat tgttttttta gtgcttattt atatctatac ataaat
//
LOCUS       DAEMHW010000094        14385 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEMHW010000094 DAEMHW010000000
VERSION     DAEMHW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.094"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(156..1034)
                     /locus_tag="KPK67_002300"
     CDS             complement(156..1034)
                     /locus_tag="KPK67_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2459258.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(1051..1368)
                     /gene="trxA"
                     /locus_tag="KPK67_002301"
     CDS             complement(1051..1368)
                     /gene="trxA"
                     /locus_tag="KPK67_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG2459259.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1518..2750)
                     /locus_tag="KPK67_002302"
     CDS             complement(1518..2750)
                     /locus_tag="KPK67_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2459260.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(2740..3411)
                     /locus_tag="KPK67_002303"
     CDS             complement(2740..3411)
                     /locus_tag="KPK67_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2459261.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3427..4263)
                     /locus_tag="KPK67_002304"
     CDS             complement(3427..4263)
                     /locus_tag="KPK67_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459262.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKPHKVKI
                     DK"
     gene            complement(4289..4963)
                     /locus_tag="KPK67_002305"
     CDS             complement(4289..4963)
                     /locus_tag="KPK67_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2459263.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(4977..5705)
                     /locus_tag="KPK67_002306"
     CDS             complement(4977..5705)
                     /locus_tag="KPK67_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG2459264.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(5832..6521)
                     /locus_tag="KPK67_002307"
     CDS             complement(5832..6521)
                     /locus_tag="KPK67_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2459265.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6529..7092)
                     /locus_tag="KPK67_002308"
     CDS             complement(6529..7092)
                     /locus_tag="KPK67_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2459266.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(7094..8062)
                     /locus_tag="KPK67_002309"
     CDS             complement(7094..8062)
                     /locus_tag="KPK67_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBG2459267.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8163..8206)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8571..9200
                     /locus_tag="KPK67_002310"
     CDS             8571..9200
                     /locus_tag="KPK67_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2459268.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNIVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQSY"
     gene            9175..9579
                     /locus_tag="KPK67_002311"
     CDS             9175..9579
                     /locus_tag="KPK67_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBG2459269.1"
                     /translation="MKIRNKATNKKKKYVRISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            9582..10064
                     /locus_tag="KPK67_002312"
     CDS             9582..10064
                     /locus_tag="KPK67_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG2459270.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNNNKIE
                     LSKFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNF
                     PSSLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS
                     "
     gene            10636..11301
                     /locus_tag="KPK67_002313"
     CDS             10636..11301
                     /locus_tag="KPK67_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBG2459271.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            11323..12255
                     /locus_tag="KPK67_002314"
     CDS             11323..12255
                     /locus_tag="KPK67_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBG2459272.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            12276..12917
                     /gene="pcp"
                     /locus_tag="KPK67_002315"
     CDS             12276..12917
                     /gene="pcp"
                     /locus_tag="KPK67_002315"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBG2459273.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(13037..13369)
                     /locus_tag="KPK67_002316"
     CDS             complement(13037..13369)
                     /locus_tag="KPK67_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2459274.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            13635..14255
                     /locus_tag="KPK67_002317"
     CDS             13635..14255
                     /locus_tag="KPK67_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2459275.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCESCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
BASE COUNT         4985 a         2233 c         1663 g         5504 t
ORIGIN      
        1 taatatagct ttgaatttta ttaataaaag taattacata tagattttaa aatactttta
       61 ttgataactt aaaaataaaa aactgtcttg aaataatttt attatttcaa gacagttttt
      121 tataaatatc tttaaaaagt taatcaatat aaatattaag ccctctttaa tttatctaaa
      181 tagtaaacag catttaaagc tgctatttgc ccttgaccag ctgcttttat ataagaatat
      241 ggttttccaa cacaatctcc agcagcaaag catccatcta tactagtctt catattattg
      301 tcaactttaa tatgaatgcc atcaatctct attccaggaa ccaatgcact tggagccgta
      361 ctatctttta taacgaatac tccatcaact tcaatgtggt tttctttaaa agatactttg
      421 tttacaagtt tatctccatc tatctgtact ggtctgtcat gtataacttc aatagaatca
      481 tctaggttat ctgaacttct cataagatta tcttttttgt acataggtat aaaataagtc
      541 tttgaagtga gttcacttaa aaaatttgct tcttctttag attcttcatt atatcctata
      601 actgctacct ttttatttct atagagtggt gcatcacatg tagcacagta gcctacacct
      661 cttcctagaa attcttcttc acccttgatt ggtctagtat actctacccc agttgcaagg
      721 attacagtag tagcttcata catatcattt ccagacatta ttgtaaagta ttctcccata
      781 gcataaatat tatttattct tttattttca attgaaatat ccatactatc tatatgtgat
      841 ttaaacttgt cttttaattc atcaccactt atatcataaa aacctaaata attatctatt
      901 gaaggagctt taacaagctt attacttaaa ttatcattac caaacatgat aattgtttta
      961 tttcttattt ttgcatttat agcagcagac aatccagctg gaccactgcc gattatagct
     1021 atatcatatc tcataatact cacccatttt ttataaatat gattttactt gcatttcaat
     1081 tttgttcttt ggcatgaatc caattaaagt ttctactggt tttccatctt taaatataat
     1141 catagttggt actgtagata cattgaattg ttgagctatt tctaagctct catctatatc
     1201 aacttttaca aatctagctt tatccttcat ctcttcacct aattctgcaa atacaggtcc
     1261 caacatgtta caaggtccac accaagttgc aaagaaatca actacaacta ctcccttact
     1321 accttcaaca ctacttctaa attcagatgt atttataact tttgccattt ttaaatcctc
     1381 ctattaaact ttattatccc tatataatta tactatgaaa tttataaata taaatttact
     1441 tttataaggt ctttttatat tttatactaa ttagtatacc cattcttcat ttctaaaaac
     1501 aattttttat atttttatta ttttaacttt tttggtattg acaattcaaa tgtagttcca
     1561 atatctacat cagaagttac atttatatca atagataagt ttaaactaat catgcgagct
     1621 attgaaagtc ctattccaga accttctaaa tctttatttc ttacattttc acttctaaaa
     1681 aatctatcaa atataagagg tatctcttct ttttttatgc ctataccagt gtcacttata
     1741 agtaaagtta cctcatcctc tatatcttct ttcaattcta atgctatctc atcacccaat
     1801 tttgtatact taaaggcatt atcaatgaaa attaatataa gctgtcttaa tttattttta
     1861 tctgttatta taactttatt cttcagttta gaattaaaca cgaatctttt ttcctgaaac
     1921 tcagcaatgt caatatagtc ttctgatatt tcttccaata acttttctaa atctatttct
     1981 tctaaattaa ctttaacaat agagtcctct ttagtaagag acaataaatc agttatcatt
     2041 tttttaagtc ttctagtttc cttcattgca gtagctattg tttcaacttc atcatttact
     2101 gtactttctg gggattttaa catactttct aattttgatg aaactattgt tattggtgtt
     2161 cttaattcat gagaagcatc ttgtataaac ttagcttgat ttttccatgc tgtttctatt
     2221 ggaattaaag cttttctagt gagatataca gccacaaagt aagtaattat tacagaaatt
     2281 aatattccaa ttataagaac agatgtaagc tgtcttaaag aattcatctc agaatctata
     2341 tttctaatta tctgtatttg gtatctacct acatctacat ttaattctct aaaagtatat
     2401 ccattttctg tgtatttaaa aaacgaattt tttttatcta gtcttctatc tggaagtaac
     2461 tcatcaaaat attcattttg agtatagtaa cttatccttc catcttcata tacatacacc
     2521 atatctttag gatctttgag tcttattggg ttcaaaaatg aagttctttt aaattgagag
     2581 actatatatt caagttcatc atttatctca ctatcaattc cactatatgt aagtccccta
     2641 aaatatgagt atataaatat agaaaagatt attaaaaaac tacctactac aataatatac
     2701 atttttatta aatgattttt agtactggta aatacattct tatttatcat cgaatatata
     2761 tcctacctta cgtttagttt taatatattt atcatatcca tatttactta gcttttttct
     2821 tagattgctt acatatactt ccactatttc aatagttgca tctgaatcaa ttccccatat
     2881 tctatcataa atctgctctt ttaataaaat tgaaccttta tttaatacaa aatactcaag
     2941 caagttaaac tgtttgtttt gaagttttat ttcttctttt tcaatataca ctcttttttc
     3001 tagtgtatct aaatataaag atttaaattc taatccgttt ctttccttta tctttccatt
     3061 agtccttaat atagcgtata ccctagctac taactcttcc atataaaaag gttttgtcaa
     3121 ataatcatta gctcctatag taaatgcttc tactttgtca tctaatgttt cttttgctgt
     3181 caatataagt acaggtgtat ctatattatt aactctcatt ttctttagga tatcaaatcc
     3241 atttatatta ggaagcatta agtctaaaat gactaaatca tatatatttt gatttactaa
     3301 gtataaagct tcttctccat cttcacactt ttcagtttca aaatgcttgc taagttcttc
     3361 tactacactt tctaaaagta tcttattatc ttcaactatt aaaaccttca taagtatccc
     3421 ctacctttat ttgtctatct taactttatg aggttttaag tcaatatcca cattggcttt
     3481 ttcttttata tcatcatacg tatatcccac tatttcaaca ctttttgcct ttgttgaaca
     3541 caatatatct atatctgtct cttctgaagg ctttaaaatc ttatatgatg gcacacgatt
     3601 tactgttaaa tattctccat tttcattaac ttctgctgtc ttaagaataa tatttcctaa
     3661 atcttttgat gatgaatttt ttacttttac ttgatacgta attccctcat ttgattcatt
     3721 tactttttta agttctgatg tagatagcac ctcatactga ctactatttt ctagcttaat
     3781 attattattt cttatattaa tagtattttc ttttaaatta acatacacaa tcttaccctc
     3841 tgcctcatat tcatatgcat aaacttctac accctttaca aaagttttat gcccacattc
     3901 tatgtaagca actttcccag gcattaaagt catgtccaac aaagccttac tatccgaaga
     3961 tataattttt ttattctcat catattcatt atatatcaat tcaacattgg atatattaaa
     4021 tgttgaatta ttttctacct ttgttgatgt atctacttta tcaccttcat ctatatttat
     4081 aatttctgat tttcctatat tcaatacttt tgcatttccc tcatctattt ttatctcatt
     4141 tttatcttct gtttgaatat caactgaatc aggctgttca tctttttcac atgctgtcaa
     4201 cataaaaata aatattgtta atataatcat tagagaaatt gattttttta ctattttatt
     4261 catattaagc acctcatata gtagtatttt atagtagatt tttatacttt ttcataaatt
     4321 ctttcaatgc ctgtgtaagt atttgatgtt tttcaaaatt cgaattacta cataatcttt
     4381 caaaatcatt ccatatttct ttatctatac gtattcttgt tgtttttaat tcatctatgt
     4441 gttcatcatc tatatgtaca tcttttttat tagatacttt tttaggcttt atattgtaat
     4501 cttttacttc taaataccaa tcatatactt cacaaagctt atctaaatct tcttgagtta
     4561 tatttatttt tctatctttc ttaggcttaa cattttgttt tggctttgca ttttttaaac
     4621 ctgtatcatt aaactcaatt tgttcaacac ttttacttaa agccttttca ttagaaatct
     4681 tttttttggt gcttactttt tttctattac ttatagtatt ctttttactt ttttcatact
     4741 ctttagcttt ctcttcttcc aagtttctat tatccaaact tttattataa tttaattcgt
     4801 cttttaatat gtatttacct ttttctgact tatatccttt tttatttaaa aaagttctta
     4861 aagtactttg actaatcttt agattttgag ctatctctgc aaatttaaat cctttgcttt
     4921 gaagttctaa aaatttgtca attctgtctt cattgttatt cattaatatt cctccgtcaa
     4981 catcttcttg tctttctttt catacttctt attatattct tcttttctaa atctactcta
     5041 tttgattcaa ttattttttg tagcatttta ttataagtga aatcatctaa attattattt
     5101 tttatataga gaaaagtttc ctcttcacat ttaataaaac aaattgatag caaccatgcc
     5161 atacccattt taacataata ttcttcactt tgtatattat tacagtattc aaatatttct
     5221 ttaatatgtt tatcatcaac ataatatatt aaaaacataa ctaatgcaaa tctaatttcc
     5281 catggattct ttgaataaac atatttttta agtatatcat acatattttc tttatatttt
     5341 ttcatagact ttaaatcaga acaaaatcca tcgcaaactg accaattatc gattttaggt
     5401 ataaatcttt ttacatagta taaagtttct tcaaaattac tatttatatt gccaattacc
     5461 ataccttgta gcctaacttc ttcatagtat gtatcctctg ctacttctaa aaattccttc
     5521 caatttcctt ggcttatttc ttttgataac tttcttaaat taggaactct aatacctatt
     5581 atattttctg ttccaggtat aagcttttta ttaaaatctc tatattttat atcttgtata
     5641 gacatcatat attctaaaag ttcattataa tccaatttat tccattttcc cctatcaaac
     5701 atcatttaat tgctccttaa ttcctttttt cttattgtaa cataaaactc tacaaaaaaa
     5761 gcttctaact attagaagct ttttcaaaaa cataagaaac ttttcaattt taaatttaaa
     5821 ctaaattaat gctaatctcc tacaccaaag tctgtattat tgtataaaac ttctatattt
     5881 ggattttcat ccaagaattt atacttacta acaaaccatt tcactaactc ttcatctctt
     5941 tttatactag aagaattatt tataaaaaca ttcactgtcg ctttttcaaa agtatttagg
     6001 ataatctcta tatctttatc tatcatctct tttgtttgtc cttttattcc aaccataata
     6061 caaggtgaat caaaatattc ttttacatct tgtggagtct taaaatttgc atttttattt
     6121 aaaatattat ttctaaaatc attgtcaaag gtttcaactc caattttaaa taccacagga
     6181 atttcaaaat actctctcat ttccttaagc ctatttttgt aagaccaatg actttctaaa
     6241 aataattttt ttatattctt ctcttttata atacacttta ttttttctaa agtatcctta
     6301 ggtaattcaa aacagcttcc agaatttatg acttcaagaa ctccatattt cccagttacg
     6361 ttttttaaaa cctttagatt taatttattc atctcttctt ctaaattaga attatcatct
     6421 atataatcac aaaaagaaca ctttccccat atacaaggaa atcctttaag cagaacaatt
     6481 tctctttgat ttttatttgt tatctcacta tatctatcca ttttctatct attttcctcc
     6541 agtcaatgat acctttaaag tttttggaac tgcatttaat cctaaattca ctaaaacaac
     6601 tgctaacagc ttatctccat aatcagtaaa tacttgggtt acaaacacac taaaaacatc
     6661 tcccagtcct aacactttca agatttgcac tatatatgat gaacctgaag aggtcatacc
     6721 tccaaataaa aatgctgaaa ttattgaact tattatagat gttggaatgg caaataaaag
     6781 tactcctagt ggagttttta aaccccttaa aaaattcttt ttaaatgcta ttccagcaag
     6841 taatccagtt gatatttgaa ctggtgcaaa atagagagaa tacacatcaa acgtaattcc
     6901 acttattaaa cttccaaaca cacctgtaac aacagcgtat ttaggtccta aaagtgaagc
     6961 tatcattata gttcctattg aatctaagta tacaggtaat cttaatccca tagctataaa
     7021 agcgcctatt atatttatag aaataccaaa agctataagt gtaatagttt ttacacatat
     7081 ttttcttttc acactatatc actccttcca cagagtctat aatgtcttca taacctttaa
     7141 aaagcctctt taaaaacatg tacataaatt tcttctcatc aacttcattt agaacaattg
     7201 cattaggatt ttttttatag aaattgaaag aatccactat agactgaccc atagctattc
     7261 catcttctac aacttctaca tatgattcaa atcctttaca tatacttcta tctataaagt
     7321 atgctactgc tagagggtca tttatcacac agccaattat tccctcctgt tcccaatgga
     7381 aatcaatata aaatctagtt atttcagtta tatactttgc catcttctta tcaagtctat
     7441 ttataaactc aataatatta ggagtaagta caatttttct agttacatct aaacctacca
     7501 tgtggatttt tttagataaa ttcttgtaaa cataatctgc tccatgtggg tctacccaat
     7561 aattaaactc tgctactgga gagcaatttc catgaatcct aaatgctcct cccatagata
     7621 caaactcatc gagattttca aatgctttct tatctttaat aagtgcttta gctatatttg
     7681 taagtggtgc aagtgctatt attgatactt tttcatgatt atgtaaagta tttattataa
     7741 aatccactgc tccatttttt gctttagctc ctacaacctt ttgataaaag ttttctccaa
     7801 taccatcttc tccatgtgta tcttgagctg ttacaagttt tctttttagt ggtgcttctt
     7861 ctcctatata tactggaata tttagtgaag aacacatttg aagtgttttt agtgcatttt
     7921 ctgcacctat atttgctgga acatttccac aacatgtggt aattccaatt acttctagct
     7981 ctggtgagtt taaagccaga agaattgcca aagaatcatc aattcctggg tcacaatcaa
     8041 ttattacttt tctcttttcc attttttatc ctccttgatt tgtggtcgag gagaactaca
     8101 catctttttt ccactttcaa aaaggtgtgt ctaagggtat taattatata ctgccactat
     8161 ataattcctt agttttttat acttggagtt ctcgaacaag tacagatgta aaatcatatt
     8221 tttagtctac ttactataga ctcttaaaaa ctatagactg ttaaaaaatt tttaaacatc
     8281 tgatttaata gctataaaat aaattttata gcataaaaga atttattata ttttaatgta
     8341 tcttattaaa gtttatttag aattatgaac caactttaat aagatgtggc gcatttaaac
     8401 cgattattat tatacaataa tatcttaaat attacaatta ataacattaa tttattatcc
     8461 ataaaaatat attaacttat attatttaat aatttattga cagtattaaa attaaattat
     8521 acaataacag tgtaaattaa cttaacaccg ttaagcaaag aggtgattat atgagtatca
     8581 aagaaagacg taaaaatgaa aaagaagaaa tgaagaaaaa gattatggat gctagcatcg
     8641 aaattataaa ccaacatgga tatgaaaatt tatcaataag aaaaattgct acaaaaatag
     8701 aatattctcc aactacaatt tatctttact acaaagacaa agcagagatt atcagcgata
     8761 tgaccaataa actttataac atagtagaaa gtaatgccat tgacattatg aataattgct
     8821 catcactacc aatagataaa caaatcatag aaattatgac tagctttata aaaactcttt
     8881 ctagtgaacc agagatggca aaatctatca tgcatagtag aatgaatatt atatttgcat
     8941 ctgaaaatac aaacaatacg cctagcaata atggtataaa aatgcttgat aaattccttt
     9001 ccattggaat tgaacaagga atattcaaaa aaaatataga taaaagctca tggatgctta
     9061 taagtgcatt gttaggattc gttttatgtg ttgttgaaaa taaactatat tctttacata
     9121 atttttctca acttatagaa aatttcttag atattttagt gggaggactt tacaatgaaa
     9181 attcgcaaca aagctactaa taaaaagaaa aaatatgtaa gaatatctat tgctctattc
     9241 tttgtattta tatgcattat tattgtaatt gtaagcaact taaagccaga aaacttggta
     9301 gtaaaagata tagatattaa taatgttaaa aatggcattt atactggaag tgctgataat
     9361 aacttggtaa aagctactgt gtctgttgaa gttaataatg gtaaaatcca aaatattaat
     9421 atcttaaaac acgaccatct tttaggtaaa cctgctgaaa aaattacaac aagtattatt
     9481 aaacaacagt ctttagatgt ggatgctata acttctgcaa cttatagcag taatactata
     9541 cgaaaagctg tagaaaatgc tttaagaaag ggagaataat tatgaatact attattatat
     9601 attcaagtaa atatggttgt acaaaagact gtgcaaatat actaaaaaat aaactatcag
     9661 ataatgtcac tattgttgat attaataata ataataataa taataaaatt gagttatcaa
     9721 aatttgataa aattattatt ggaagttcta tatatgttgg ttcagtttca aaaaaaatac
     9781 aagtgctatg taatgataat gtagagttat taaacaaaaa acaagttggc attttccttt
     9841 gctgtggctt ttctgaacaa gcagataaat atttaaaatc aaattttcca tcatccttat
     9901 tagaaagtgc aaatgctatt ggaatttttg gtagtgaagc tcgattagaa aaaatgaaat
     9961 ttttagataa acttattatg aaggctgttt caaaaggtaa ttatgacagt tttagaatat
    10021 cacaggataa tatagataat tttttaataa atcttaatag ctaaagtaaa gctaattatt
    10081 tctatgataa attcttttat ttataagtaa tattgctcaa aatagtcttt aaactttatc
    10141 aaaattttat ttttcttagt gaaatataat gaaactagca cttaatatat actttattaa
    10201 tcataacaaa tatgttttat taattataac aaaaagagag tgtctaatga actaaaaaat
    10261 tcattagaca ctctcttttt gtatgaaatc aaatatattt ttatcatttc aaaagtgaaa
    10321 aatacacttg tctctatgtt tggcacattc tctttttata atataatgtt taatttaaaa
    10381 ctactatttc aaatattgac atatacttaa actatagact tacaaaatca attcatatta
    10441 gcttaaaata taatctttgt tattcaaatt cctatacaca accattattt actttgatac
    10501 tttaaaacat cttccctatt ttttattaaa ttttataata atagtcctat taacgcaaat
    10561 actataaaaa gagtttaact ttatccatta aataaattta aattaaaaca tatagaaatg
    10621 gaaggagaac aatttatgat aaaactaatc ggtatattga tagttgtaat tggatttata
    10681 cttaaaattg acacattatt tactgtatta ttagcaggag tggcaacagg aattgttgca
    10741 ggattagatt ttaatcaaat ttttacaatt ttaggagatt cttttgtctc aaacagaggt
    10801 gtatcattat tcatattaac attgccagta atcggtgtat tagaaagata tggtttaaag
    10861 caaagagctg tttctttaat agaaaaatta aaaagactta caacaggaaa agtacttact
    10921 atatacatga tagctagaca aatagctggt gctctatcaa taagaatgag tggacatcct
    10981 caatttgtaa gaccacttgt taatcctatg gcacaagctg ctggtttaag taatagtgat
    11041 gaactaaaag aatcggatga agaagccata aaggcattat ctgctgcatc agaaaactat
    11101 ggaaattttt atggtcaaaa cttatttgca ggtagtagtg gagtattact tatagcatct
    11161 actctaactc aatttggata caatgtaact ggacttaata ttgtaaaggc tagtactatt
    11221 atggcagtaa tagcacttgt agtggctaca atccagttta ccttatatga taaaaaatta
    11281 aacaaaagtc ataaaaaata gttttattta ggaggaataa aaatgcaaaa ttttataaac
    11341 atatcattag aaatctttta cgcactaatg ggatttttaa tgattgttat agcatataag
    11401 tcttttacta ccataaataa caataagaaa tatggtacat cattattttg gatacttata
    11461 tcactgccat tcatatttgg tagattgatt ccagccaata taataggaat aatattaata
    11521 ttatctagtt tattaacact ttctaaacaa gttgtatttg caaagtatga agaaccagat
    11581 gaaaattttg gaaaagaaca agcagacaaa cttaaaaata aaatatttat accatcatta
    11641 atattagcat ttgctgcagt agtagtagct atgagccttt ctaattttga taattcttct
    11701 caatttgcaa taggtgtggg ttcaataatt gcattaatat cagcccttat aataactaag
    11761 gcaaaacctg ctacatcagt tcaagatggc tcaagattac ttcaacaaat gggacctgct
    11821 agtatgttac cacaactttt agttgcattg ggagctttat ttactcaagc cggagtcggt
    11881 gaagttattt caaccatgat atctggtgta gtccctgctg atagcagatt atttggtgtt
    11941 atagcttatg tattaggtat ggttatattt accatgataa tgggaaatgc atttgccgca
    12001 ttttcagtca taacggcagg aattggtatt ccttttgtat tgtctcaagg tggaaatcct
    12061 gctataattg gagcattagc attaacagca ggatattgtg gaactctact tactccaatg
    12121 gctgctaatt ttaatatagt tccagcagcc ttgctagaat gtaaaaatga ttatattgtt
    12181 ataaaatatc aagctccagt tgctttggta ttaattatag cccacatatt agtaatgtac
    12241 tttttaggat tttaatgtaa ttaggaggta aaataatgaa aattttgtta acaggatttg
    12301 acccatttgg tggagaacca ataaacccag ctcaagaagc agttgaaagg gtcaataata
    12361 atataaacgg tgcagaaatc ataaagataa ctataccaac agttatgaca aaatctgttg
    12421 aagctataga taaagctatt caagagcaca atccggatat agtaatatca gttggacaag
    12481 ctggaggtag atttgacatt acacctgaga gagttgctat aaatatagat gattttagaa
    12541 taaaagataa tgagggtaac caggttatag atactattat aaaagaagat ggtgaacctg
    12601 catatttttc aaaattgcct gtaaaagcta tggtaaaaca tatgaatgaa aataaaattc
    12661 cagctagtgt ttcaaacact gcaggaacat ttgtttgcaa tcatgtgatg tatggaattc
    12721 tttatatgat agacaagaaa tatcctaata taaggggtgg atttatacac atcccatata
    12781 caacttcaca agtaatagat aaaaaaaata caccattcat gtctttagaa gaaatagtaa
    12841 aaggcttaga attagctata gaagcttgta ttatatataa agaagatgta aaagaaattg
    12901 gtggtgaaat ttcataataa attttttagt atttttataa attaaaaata ctaactatta
    12961 atttaaatat agactgtgag tagattattc aaacaatttt actcacagtc tttttatggt
    13021 atctacagat acaaaattaa ggtctattcc tctctcccca ctcacacatt gcatctaaaa
    13081 ttggaatcag agattttccc ctttctgata gactatattc taccttagga ggtatttgag
    13141 gatattcagt acgaataatc aagttatctg cttctaattc ttttaatgat aaactcaaag
    13201 ttttatagga gatagtattt atataacgtt ttaatgcatt atatcttaca acttcaaatt
    13261 ctacaagaca gtaaagaatt atcatcttat atttaccact aatcagtgaa agtgtataac
    13321 taaatcctgt ttcacttaaa tctgtatttg atatacagtc tatactcata tattacactc
    13381 tccttttggt tagtattgaa caaaaatgtg cgtacttgtt tttagaataa tttataataa
    13441 aattatagtg taagaataat tgaaagtcaa ttttataata ggaggtgtat ttactaaaaa
    13501 aaaagttatt ttaaatagga ttctaagatt agacatttct atataaattg ataatattca
    13561 tgaaaatcct attttataaa aaataatatc tcttagaaaa tctattaaat aaaattcatt
    13621 ggaggaaatt tataatgaaa gttttattga taaacggtag cccaaaccaa tatggatgta
    13681 cttatacagc actaaatgag attactaaag tactgagtaa acacgatatt aaaacagaaa
    13741 tactgtattt aggtaaagaa acaatcccag gatgtattag ttgcgaatcc tgttttgaaa
    13801 ctggaaaatg tattcgaaat gataaagtta atgaattgat tgaagatctg gataatattg
    13861 atggtattat tattggttct cctgtttact tttctagtgc aactggtcaa ttaacttctt
    13921 ttcttgaccg tctatttttc atagctggga gtcgcatggc tacaaaatta ggtgcttcag
    13981 ttgtgtcatg tagacgtggt ggtgcaagtg caacatttga ccaactaaat aaatattttt
    14041 caatcagcaa tatgcctatt gtatcatcac agtattggaa ccaggtacat ggctttactc
    14101 cagaggatgt tatgaaggat gaagaaggat tacagactat gcgaactcta ggagaaaaca
    14161 tggcatggct tcttaagtgt atatcagcag gtaaaaaagc tggaattaaa gaaccacaat
    14221 atgaagaaac aattatgact aattttataa aataaataaa aaatgtacta atattaatta
    14281 aaacaaaaca ctaatttata aataaaataa gcataaatgt aggataaaaa gcactaaaac
    14341 agaggtattg aattttatat caaacctctg ttttattatt acatt
//
LOCUS       DAEMHW010000095        14373 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEMHW010000095 DAEMHW010000000
VERSION     DAEMHW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.095"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            231..1310
                     /locus_tag="KPK67_002318"
     CDS             231..1310
                     /locus_tag="KPK67_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG2459276.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYCSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYMKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="KPK67_002319"
     CDS             1634..2002
                     /locus_tag="KPK67_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG2459277.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="KPK67_002320"
     CDS             2119..2922
                     /locus_tag="KPK67_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2459278.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3312..4202
                     /locus_tag="KPK67_002321"
     CDS             3312..4202
                     /locus_tag="KPK67_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2459279.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
     gene            4616..5059
                     /locus_tag="KPK67_002322"
     CDS             4616..5059
                     /locus_tag="KPK67_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2459280.1"
                     /translation="MIEIKTIEINKKGQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYLSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            5231..5401
                     /locus_tag="KPK67_002323"
     CDS             5231..5401
                     /locus_tag="KPK67_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459281.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            5884..6255
                     /locus_tag="KPK67_002324"
     CDS             5884..6255
                     /locus_tag="KPK67_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2459282.1"
                     /translation="MGIKLVDSELKVMDVLWKEGDSTAKHISDVMTERYGWNINTTYT
                     LLKRCVKKGAVERQEPNFVCHALVQQEQIQALETDELLNKIFDGSVDKLFASLLSRKK
                     LTTEQIEKLKCMVDKLGGNEK"
     gene            6252..7832
                     /locus_tag="KPK67_002325"
     CDS             6252..7832
                     /locus_tag="KPK67_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG2459283.1"
                     /translation="MTLLQMSISAGIMIFLITVIRALTINRLPKKTFLILWGIVLIRL
                     LVPFSWPSPLSVYSLINHSYTVVGETTTVNVLPLISSTNIVAGSNTGISPFVLVWGIG
                     ATICALYFIIVYIKCRLGFMNSQLVENVFITQWLKEHKSKRPISIRQTFSISSPLTYG
                     IFRPVILVPEQTEWTEIDRLQYILTHEYVHICRFDAITKLLLIIALCLHWFNPFVWVL
                     FILANRDIELSCDEKVIQIFGENIKSAYALTLIGMEENKSGFDSLCNNFSKNAIEERI
                     ESIMKMRKPTVITLLIMILLVGSFTVVFATSSIKKEKKFTTQNSSIEEKNTMNESVNQ
                     TITSRNKTNGTMQQTVTNRNEINGTIVYDKVEVRHYSDGGEPYVFWQRTNATEQSIAK
                     IQFGMLAYDKNGNALNLPWKVEDSSAGYSYDVLCEWEPKSLKSKQQADSNDGNGEGGW
                     SLGWTIDMFNTDTPELRELFEQVEYVLCCDKQITFENGEVWNNPDYNNWLETYKGKTV
                     DIAILQGYYPNELQVKFK"
     gene            7825..8358
                     /locus_tag="KPK67_002326"
                     /pseudo
     CDS             7825..8358
                     /locus_tag="KPK67_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888423.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8538..9026
                     /locus_tag="KPK67_002327"
     CDS             8538..9026
                     /locus_tag="KPK67_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBG2459284.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLGIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            9112..9519
                     /locus_tag="KPK67_002328"
     CDS             9112..9519
                     /locus_tag="KPK67_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459285.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            9520..9738
                     /locus_tag="KPK67_002329"
     CDS             9520..9738
                     /locus_tag="KPK67_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2459286.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            10166..10471
                     /locus_tag="KPK67_002330"
     CDS             10166..10471
                     /locus_tag="KPK67_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459287.1"
                     /translation="MPGMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            10879..11568
                     /locus_tag="KPK67_002331"
     CDS             10879..11568
                     /locus_tag="KPK67_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2459288.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIRYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            11568..12485
                     /locus_tag="KPK67_002332"
     CDS             11568..12485
                     /locus_tag="KPK67_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2459289.1"
                     /translation="MELFLVLISIVLFVLYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVKKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            12558..13484
                     /locus_tag="KPK67_002333"
     CDS             12558..13484
                     /locus_tag="KPK67_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459290.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFGKKVAETDFEILKKIGTIIENPYFYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEIYKIFI
                     KNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            13481..14161
                     /locus_tag="KPK67_002334"
     CDS             13481..14161
                     /locus_tag="KPK67_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2459291.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLVFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
BASE COUNT         5512 a         1542 c         2517 g         4802 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaatttat atggtttata gtttaacagt aggaaatatc
      121 gtatgagtaa gaatatcgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcaaat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagtatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg gtcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt gctcttcaga atttgctgta agagcattta
      601 tcatcaagga tgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggct gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacctg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgcaaagg attggtatgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgc agaactctgc taaaaaaagg caatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata tgaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt accacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagcc atataaatta gaatttgtag
     1621 aggagaaatg taaatggatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaagatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccag atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggat taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaattgttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgatgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaactaca tactcattag
     2941 gtaaattatg tagtatatgt gtttatagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacattta aaaagtggat ttgtccagag cataatacaa agcatgatat ataaattttt
     3061 aagcgaaata catcaaaaga aggattagaa ttagtggaga cagaacagct attatagatt
     3121 ataaataaat agttttaaag gaggggtact acgaatatag aggaaggtaa atcatacgtt
     3181 attcaaggat tataaatgat tagtttaaaa gtactatcgg ataggtctct gcatatttaa
     3241 accttagtaa aggtagttaa agtagtagtt taaaaactaa aaaattatat aaaggaatta
     3301 ggtataagaa aatgattgag agtataccag caaaacaaat tttacaaaaa gtgaaatttg
     3361 acaatacaag atggtttgga attgactata atatgaatct ctatcgtggt tgttctcatg
     3421 gatgtatata ttgtgatagc cgtagcacaa tatacaacat agagaatttt gacaaagtta
     3481 gatataaaga aaatgtgata gagattttaa gtaaagaatt gcgttcaaaa agaaaaaaag
     3541 gggtagtagg cattggagct atgtctgaca catataatcc ttttgaaaag caactttgta
     3601 ttacaaagca ggcactagat ttgatttcag aaaatcattt tggagtgtct attgatacca
     3661 aaagtagttt ggtggttaga gatataccga ttttgcagaa aattaaaaaa aataattctg
     3721 ctattgtaaa gttgactata accactgcaa atgatgaatt atcaaagaaa atagaacctt
     3781 atgtaaatcc aagttctgta cgatttgagg cagtgaaaga acttaatgat tctggaattt
     3841 tttgtggtat attattgact cctatgcttc catttttgac tgataataaa gatgaaatac
     3901 gagcaattgt agaaaaagca cacaaggcta atgcaaaatt tatttattgc atgtatggtg
     3961 taacaatgcg tagtggtcag agagagtttt tttatgagca tttaagagat atatcaccaa
     4021 aattggtact taaatatcag aaaacatatg gtttaaacta tgtatgtact gtacaaaata
     4081 aagattgttg tgagaagttg ttaagagaag aatgtgcacg ttatggtatt ttgacggata
     4141 tgaaagagat tataaaagcg tacaagaggt cggagtcata tattcaaata aaattttttt
     4201 aagataggct ttggatgaga tataatagtg gtgattgatg tgttgatatt atagttgatt
     4261 gttagagagg atattataga tgaatattag tattgatatt ttaggtaatt gctagagctg
     4321 atattataga tgaatattaa tattgatatt ctaggtaatt gctagagctg atattataga
     4381 tgaatattag tattgatatt ctaggtaatt gctagagtta gtattataga tgaatattag
     4441 tattgatatt ctaggtaatt gctagagctg atattataga tgaatattaa tattaatatt
     4501 ctagggaatt atcactatat tttaaaaaag taaaaatata tatcaaaatg aattaaataa
     4561 gtactgtaag aagtttgcta aattaaggtt tgaataaagt gagggtggag aacttatgat
     4621 agaaataaaa actatagaaa taaataaaaa aggtcaaatt gaccttttga tgttagccga
     4681 cccatgtgaa gaaatgattg ataaatatct caataaagga actatgtacg cattgtatga
     4741 taataaagaa cttacttgta tagcagttgt aaatgaaatt tccaaagaaa tatgtgaatt
     4801 aaaaaacatt gctacatatg agcattttca aaatatgggt tatgcctcaa agatgattta
     4861 tcatctttta gatgtttata gtaaaaaata tttatcaatg gttgttggta catctgaaag
     4921 cggagttcct ttttatgaga agtttggatt tgtttattca cataaaatta aaaacttttt
     4981 tgtagataat tatccagaac caatctttga aggagaatta caatgtgttg acatgttgta
     5041 tctatcatat aacttttaga taggataata tttttaggtt aatatacata atttattcat
     5101 ataattatcg gaattataat ttataaatta tgtataataa gagtataatt tatttagaag
     5161 ctgttatatt gtattagatt caaataagat atataataac ttgtttattt tgaattttgg
     5221 aggattaatt atggattcaa ataataatac tataaaatca actgttaaaa agggtatttc
     5281 ttttggttct tgtttagcaa tgattatttc ttatactgca tggaaatcta ttccatgggc
     5341 tatttttcat ggcttaatga gttggatata tgtactttat tattgggtta agtatgcata
     5401 gattaaatta ataaataagt atgtaatatt gtgaagtttc catcacttga ccaaggtaaa
     5461 tatacaaaaa taattaaaaa tagaatatca aacattgaat aacaccacct aaatagagac
     5521 tacaggtggt gttttcatgt aatgatggaa agtaggaaaa atccctcttg ctttagggta
     5581 tattggcttg atgtcatttt agggtatgac taaaaaggcc atagagactc caagtggagg
     5641 ggacagttaa gtctgataaa gaggttgagt agactaggaa caattagact tctaccacca
     5701 ataagttcaa atgacaaaaa tttgcaagta tatagacact tgaattgctt gccatttagt
     5761 gcaagtctgt tagactttta gttatttagt atataataca attgtagtat tgacatttcc
     5821 ataagacaat ggtagtataa agcatagata atactatagt cttacagtgg gaggaatata
     5881 caaatgggaa ttaaattagt ggacagcgaa ttaaaagtaa tggatgttct ttggaaagaa
     5941 ggggattcta ctgccaagca tatttctgat gtaatgaccg agcgatatgg atggaatata
     6001 aacacgactt atacgctttt aaaacgttgt gttaaaaagg gagctgtgga acgccaagaa
     6061 cctaattttg tgtgccatgc gcttgttcaa caagaacaga tacaagcatt agaaacagat
     6121 gaacttttga acaagatttt tgatggttct gttgataaac tttttgcatc tttgttaagc
     6181 aggaaaaaac tgactacaga gcagattgaa aaactgaaat gtatggttga taagttaggg
     6241 ggtaatgaaa aatgacttta ttgcaaatga gtatttcagc aggaataatg atttttctta
     6301 taacagtaat tagggcactt actattaatc gattacctaa aaaaacattt cttattttgt
     6361 ggggaattgt tctgatacgg ctattagtgc ccttctcatg gccttcacca ttaagcgttt
     6421 actctcttat aaatcattca tatactgtag ttggagaaac taccacagta aatgttttac
     6481 cactcatttc ttctacaaat attgtagctg ggtcaaatac tggcatttcg ccatttgtgt
     6541 tggtatgggg aataggggca acaatatgcg cattatattt tataattgtc tatatcaaat
     6601 gccgtctagg atttatgaac tcacagttgg tagaaaatgt atttatcact cagtggctaa
     6661 aagaacataa aagcaagcga cctatttcaa tcagacagac attcagtatt tcttctcctc
     6721 ttacctatgg aatttttcgt cctgtaattt tagtaccaga gcaaacggag tggacggaaa
     6781 tagatagatt gcaatatata ttaacacatg aatatgtcca tatctgtcga tttgatgcga
     6841 taacaaaact tttattgata attgcgttgt gtctacattg gttcaatcca tttgtatggg
     6901 tattgtttat actagcaaat cgtgatattg aactttcatg tgatgaaaaa gttatacaga
     6961 tatttggtga aaatataaaa tctgcttatg cccttacttt gattggcatg gaggaaaata
     7021 aaagcggttt tgattcatta tgcaataatt ttagtaaaaa cgctatagag gaaaggattg
     7081 aatcaattat gaaaatgagg aaaccaacag tcataactct attgataatg atattacttg
     7141 ttggaagttt cacagttgta tttgcaacat catcgataaa aaaagaaaaa aaatttacca
     7201 cgcaaaactc ttctattgaa gaaaaaaata ccatgaatga atcagtgaat caaactatta
     7261 caagtagaaa taaaacaaat ggtacaatgc aacaaactgt tacaaataga aatgaaataa
     7321 atggtacaat tgtttatgat aaagtagaag tgcgccatta cagtgatggt ggggagcctt
     7381 atgttttttg gcaaaggaca aacgctacag aacaatctat tgctaaaatt caatttggta
     7441 tgttagcata tgataaaaac gggaatgctc ttaatttgcc atggaaagtc gaagacagca
     7501 gtgctggcta ttcctatgat gtattatgtg agtgggaacc gaaaagtctt aaatcaaaac
     7561 aacaagctga ttccaatgat ggtaatggag aaggaggatg gtctttaggt tggacgattg
     7621 atatgtttaa tacggatact ccagagttaa gggaactatt tgaacaagta gaatatgtgc
     7681 tttgttgtga taaacaaatt acatttgaaa atggagaagt atggaacaac cctgattaca
     7741 ataattggct agaaacctac aaaggtaaaa cagtagatat agcaatatta cagggctatt
     7801 atcctaatga attacaggtg aaatttaaat aatgactacg attaaattgc aagttttata
     7861 gatgatattc tcaaagatgc tccatatgct aataggaata tcaaaccatt cactcaaata
     7921 aaaagaatcg ttagagttga aactgactta acaattaatt acatagaact tccaaaaatt
     7981 cgtaaatcca gttgtatagc aaacagagtt ctaatatggg gcaaacttat tgataatcct
     8041 tctcatatcg atattagaat actatctaaa acagattatg ttattaaaag agcacttgat
     8101 agattaaaag aattaggcag taatgaggac tatttattaa atttaaagag agatgcgtat
     8161 attatgaata aaagtagaaa ttttaaagta gaaatttttc aagaaggtgt tgaaaaaggt
     8221 attgcaaaag ggaaaagaga agagaaattt aatatcatca taaaattaaa aaataaagga
     8281 tataatttga gtgaaatatg tgatattata gatgatttga ataaatcaga ggttgaaaaa
     8341 atctataatc aaaactaaca ctataattga gatggcacta gataaatata aagcttcctc
     8401 ttttaaattt aaattaatat aaaaccttgt tatttaacta tagtagatac aaagagatat
     8461 ataagctaag gagatgattc aaaataaaag tttgaaccat ctccttctta gagaaaaaca
     8521 aaggggttga tttatatatg aaattttata ctatacagac tttagaattt tggaataaca
     8581 ataaaaacaa taaatatcta tcaaatgatt attcttttat attagaagac tttgatattc
     8641 catacaaatg gatgtatagt caaatgacaa atagaatata tgaatttgat gatagtatga
     8701 tatgggtttg gacgaagaga cctgatttaa gatgtagtgg ttatttagaa aaaggaaaaa
     8761 aaggtgtttt acttggaata gaaataagtg aagacaatgt gttattatca gaatttgatt
     8821 tatggcattt acctttaatg gatattactg tagagcttta tgaagatgaa caaattgaaa
     8881 agaaggtaag ttgggaaaga atatttgact tagaactgtg ttcaaaatta aatgaagtta
     8941 atatagaaga tttgacattg caaggagtga catctaaagt gcctctatct aatgtaaaat
     9001 taataaaaga atttgttgca aaataatatt tttattgaaa tttattcaaa aaagttatat
     9061 atttataaca agaggtgtta taaatacata actttttaag ggggatttta tatgtctaaa
     9121 caacaaactg gtattaaatc aacacttatt tcagaaatat tgggaatttc ttattgtatt
     9181 ggagctacat tagtaaataa gtattttttt aaaggaagta gtattgaata tcttcctata
     9241 attatttggg tattagctat gggagctcag tgggtctata ataaaaagta tgatgattta
     9301 attgatgagg catcaaaatg tatactatca agagttaatg atattgcaat aaaagtattg
     9361 tttttttctg tagctatagt atctatattt cttgtaactt tggacataaa tgtgtcaaat
     9421 ttaaatatag gaatgtcttt attagtcatt ttgtttattc aatccttgtt gaagttaatt
     9481 ctatttatat attttgacag aaaggggata tacaattagg tgtcagtatt actaaagaca
     9541 aaaattaaat cctatagaga aaagaacctt atgacacaag gtgaacttgc aaaatcagta
     9601 ggagtaagac gtgaaactat tgtacattta gaaaatggtc gctacaatcc ttctttaaaa
     9661 ctggcaatgg atattgctaa ggtatttgat acaactgtag aagagttgtt tgaatttatt
     9721 gaagaaaata atatttaaat ttcatgtatt acttatgcat tagagaatga attaattata
     9781 ggtaaaaaac caagcttata gattagagaa taaatgactt atagatgaaa aaccaagcta
     9841 atggattaaa gaatgaatga tttatagatg aaaaagaaag cttatagata agaagtccat
     9901 gcttataagc cagaaaatga atagtttaat agattaaaaa atacatattt gtgaattata
     9961 gaatgaataa ctccataaac tagaaaattt ataataatat ctatatgaaa attagaatta
    10021 ttttgtcaca aaaaaattaa gttaaataaa tatgaaatta cttccaaata tacttccaaa
    10081 aacatgtaat cgcatatata atataattat aaaaataata aacattttta tagtagtagt
    10141 taattattaa taaagagggg tcaaaatgcc tggtatgatt atggaagttg taggagtact
    10201 atttatagct attggattaa tcttgacatt cattgaaata aaaggaaatt tcccttcttt
    10261 ttatggtttt ggtcaaaatt ctatagggaa aaatgctgta ggtattatag cagcaggaat
    10321 tgctttaatt ttctttagaa atggcataga tagcatagat tctatagcta cttatggtgt
    10381 atttttagca atagaacttg ttgtaatagt tgcatatttc ttaataagaa aaaaatttac
    10441 taaagaagat aaacatttaa gaactaatta actaaattat ctactataaa attggaaaat
    10501 taaaaacaaa taaaagtttg tttttaatta tttaaaatcc aaatttgatt atttttaaag
    10561 aaactatgtt ctgtataaaa ctagaatgtg gtttcttttt tatgtataaa atatttttta
    10621 gcaagagtta aaaggtgttt tataatataa aatagttaat tttagatgtt aataaaagaa
    10681 aattatgtat atttcagata aagataagag agtatactat attttaatta ttttttcaat
    10741 aaagcttatt gaaaagaact ttaacttaga agagtatcac caaattgcag atacaatatc
    10801 ataagcagtt atttattgac aacccttttt aataaatata tcatttgaag tatactgacg
    10861 aaaaggagtt gaataataat ggacatctta ttggttgaag atgataagaa tattcgagaa
    10921 atggtatacg attttctgat tagtgagaat tactctgtta aaactgccat tgatggaaat
    10981 acagctattg aaatatttaa aaattattca tttgatatta ttttactgga cttgatgtta
    11041 ccaaagtcta gtggttttga agtattaaaa tatatacgaa gtgtgagtgt tgttcctgtt
    11101 attattacta ctgcgaaaga tagtgacaca gataaaatgc tagggcttaa tcttggtgca
    11161 gatgattata ttacaaagcc tttttcaatc gtagaattgc ttgcaagaat taaagctaat
    11221 attagacgtg caacagtgta tagcagttca agaaactctc aagatatggt tattcggtat
    11281 aaagatttga ttataaacat atcaaaatat gtagtttcaa agaatggaat tgacttaaac
    11341 ttaacacata cagaatttga aattgttaaa ttactagcga ctaatcaagg acgtgctttt
    11401 tcaaaggaac aattatacaa cactatttgg aaagaacctt attatggaaa tgaaaatgtg
    11461 ctaaatactc atattaacag gttaagaaat aaattgtctg atggtataga aaaaaataat
    11521 tatattaaaa ctctttgggg aattggctac aaaatggagg aagaataatg gagttatttt
    11581 tagttttaat ttctattgta ctatttgttc tctacttaaa agaacgaaat aaatcaaaga
    11641 aaatggaaag tgattataag tatatcaatt caagacttcg tgacataaca aatgatgatg
    11701 aaattaatta tattttagta ccatctgata tttcgattgt aaaagaaaca gcagtagata
    11761 taaataaact tcttgaaaag ttttacagca ggcaaattaa ttataataga agtgaaaaag
    11821 cgatattgca aatatttaca aacatatcac acgatttacg aactcccatc acagtattaa
    11881 agggttatat tgaaatgcta tatcttcaaa gtcaaaaaga agaattatct tcttcaatga
    11941 gagcaactgt gaaaaaaatg caaacaaatt caaatgagct tgttaattca ataaataacc
    12001 tgtttaatat ggcaaaaatc caatctggag atatgatatt aaatcttcaa aaatccaatc
    12061 taacacaatt atgtcatgag attattttag agttttatga tttgctagat aaagaaaact
    12121 ttaatgttga agttaatatt gaagatagac ctatatatgc aaatgttgac acagaagcat
    12181 tacaacgtat actcaaaaat ttaattgata atgctataaa atatggttca gatggtagat
    12241 ttttagggat ttcactatat gaaaaggaaa aacatgtttt tatagatatt gaggataaag
    12301 gttcaggtat ttctgaaact gaaaaagagc atattttcac aagagcttat actgctgata
    12361 gaaaaaaagg caatggttta ggtttggcta tttctcaagg gcttgcaaac tctatgggag
    12421 gttcaattta tgtaaatagt agacctaatg taaaaacagt ttttacggta aaattgaaaa
    12481 gttagaaaaa agttagattt aaaagagaga aaagataatt ttattttgta tactgttttt
    12541 agaaaggagg aagtatgatg aactatatca tagagaccca tcaattaaag aaaatatata
    12601 aagataaagc tgttgttaat gctgttaata ttcatgtgaa aaagggagaa atttatggat
    12661 ttgttggacc aaatggagca ggtaaaagta cagtaatgaa aatgttgcta aatcttgtta
    12721 agccaaattc tggagaaata gttatgttcg gtaagaaagt ggcagaaact gattttgaaa
    12781 tattaaaaaa gattggtact attattgaaa atccttattt ctatgagaat ttgactgcta
    12841 aacagaatct tgatttacac tgtgaatata tgggttatta taacaaagaa catatatcag
    12901 aggtattgga atgtgtcgga ttgtcaaagc aatcaaacaa gaaagtttca aaatattctt
    12961 tagggatgaa acaacgtctt gcaatagcta gagcaattct tactaaacca gaattattga
    13021 ttcttgatga acctataaat gcactcgacc cagaggggat aagagaaatg cgagaattat
    13081 tccgtaagtt aaatacagat tatggaataa ctatatttat ttctagtcat attctttcag
    13141 aggtagaaca aattgcggac actattggaa ttattcaaga tggtaaactt attaaagaaa
    13201 tttctatgtc agatatacac aaatatcaaa cagattatgt tgaagtagat gtagataatg
    13261 tggaacttgc tggatacttg ttagaaaaag aatttggaat aaaagacttt aaaattactt
    13321 cggaaagttg tattgaaata tatgatttac gaaaagatgt aaaagaaata tacaaaatat
    13381 ttatcaaaaa tcaaattggg ataaatagca ttggacgtaa acaaagttct ttagaagatt
    13441 acttcttcca aactacaggt acaggaggta aagaaacact atgatacatt taatgaaact
    13501 agaattaaaa aaagttgggc taaaaagata ttttttattt tcaatgctgg ggattttctt
    13561 atgtatgttt tttgtcttta ctggattgaa tgataaatct acttcaatat atgattatga
    13621 tgttacattt aggacaatca gtttagtttt ctgtttttac tatattatat tatttgctgt
    13681 gttgaatgtg gcatatataa taaatgaata tacaaataaa acaatcttga ttttattctc
    13741 ttatccactt gaccgtaaaa aacttatttt atcaaagtta cttttgatta cgactcttat
    13801 gatatcatca atgatagttg gatatatctg ttgttttaca ttcgttgttg tgttggataa
    13861 gtattttaat ttagttattg gagattttca tatttcaatt ttatcttatt ggataccagg
    13921 agcaattaaa agtattattg tattttgctc attaggtata tggacattcg ttgttggaat
    13981 gataagaaaa tcaatacctg ttacccttgt cagttcaatt atttttatca atttaagaca
    14041 atttatttta gctggtacaa atacaactca agacagtttg atttctgttt ttattatagt
    14101 tggtataact gtgattggta tatattatat actaaatcat aaagttactg aaattgacta
    14161 gatactgtgg ctaggaaggt agttattaat tgtatcttct tgggataata atgatttgct
    14221 attatatcag ctattattaa tattattaga gttttaataa tattaataat agctatatat
    14281 tttttcttct tatttataaa tttaatgatt tatagtcata aaatgaactc taagaagaga
    14341 taaaaatacg ttttatatgt ttttagatta ata
//
LOCUS       DAEMHW010000096        14372 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEMHW010000096 DAEMHW010000000
VERSION     DAEMHW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.096"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            355..1308
                     /locus_tag="KPK67_002335"
     CDS             355..1308
                     /locus_tag="KPK67_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBG2459292.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            1346..2308
                     /locus_tag="KPK67_002336"
     CDS             1346..2308
                     /locus_tag="KPK67_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBG2459293.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            2333..3319
                     /locus_tag="KPK67_002337"
     CDS             2333..3319
                     /locus_tag="KPK67_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBG2459294.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEETIKSLIVR"
     gene            3332..4378
                     /locus_tag="KPK67_002338"
     CDS             3332..4378
                     /locus_tag="KPK67_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBG2459295.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            4545..6275
                     /gene="lpdA"
                     /locus_tag="KPK67_002339"
     CDS             4545..6275
                     /gene="lpdA"
                     /locus_tag="KPK67_002339"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG2459296.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDTV
                     KENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLYRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     NAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            6693..9641
                     /locus_tag="KPK67_002340"
     CDS             6693..9641
                     /locus_tag="KPK67_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2459297.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            9619..10083
                     /locus_tag="KPK67_002341"
     CDS             9619..10083
                     /locus_tag="KPK67_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2459298.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVNLSEESVSDLLNSKLKN"
     gene            10164..10466
                     /locus_tag="KPK67_002342"
     CDS             10164..10466
                     /locus_tag="KPK67_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2459299.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            10528..11892
                     /locus_tag="KPK67_002343"
     CDS             10528..11892
                     /locus_tag="KPK67_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG2459300.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVIFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            11927..12400
                     /locus_tag="KPK67_002344"
     CDS             11927..12400
                     /locus_tag="KPK67_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459301.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            12572..13222
                     /locus_tag="KPK67_002345"
     CDS             12572..13222
                     /locus_tag="KPK67_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG2459302.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            13396..14328
                     /locus_tag="KPK67_002346"
     CDS             13396..14328
                     /locus_tag="KPK67_002346"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG2459303.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
BASE COUNT         5910 a         1502 c         2687 g         4273 t
ORIGIN      
        1 aatttttaga gaagcgaata ataaaaatat aagaagactt taaatgatga aatggttggt
       61 tttttagaaa tgatttaata agttattgtt atttaaagca ttataagtgg acaattaatt
      121 tttaatttat ggagatgata tttaacaatt agaagtaata tataatatgc actaagaagc
      181 aatttttcaa ctatttatga aggtggtggt acaagaaaag tatcaccttt tttttgttat
      241 acaaaaaaac agttgacttt ttatatgaac cccttctata atgttactga aattaagtaa
      301 aatgattgaa caaatgtaaa gataattgaa aagcttgatt ttggaggaaa gcaaatgaaa
      361 aaaattggag acttaagatt aatgatgaag tgttgttcac tttattacga agataatttg
      421 aatcaacagg aaatagcaaa tcaattggga atatcaagac caacaatatc aagaatatta
      481 aaagaagctt ttgaacaagg aatagtaaaa atacagatag ttgatgtttt gaaaaatgat
      541 taccaaaaaa tagagagaag tttggagaga aaatataaat taaaagaagt aatagtagta
      601 gatgataaac aggatgcact aacacaaaaa caagaactag caagagctgt atctgaatat
      661 ttaactagag tagtaaagga aaatgatata ataggtgttt caataggaac aacactaaaa
      721 gaaataccaa gatatgtgga aaaaagtaac tgtaaaaatg ttacatttat acctctatta
      781 ggagggattg gagacaatga aatagatatt catgcaaatc aaatagcagt aagtttagcg
      841 agggcttttg gaggtgattt taaattatta catgctccag ctgttatgtc tgacttgtct
      901 acaaaagaga aactttgcaa agacgaaaaa attaaagaag tactagattt aatagataaa
      961 acaactatag caattgtggg tataggaaat cctatgagtt taaactcaac aataatggct
     1021 tctggataca tgaatgaagc agatatagaa gatttaaaaa aatacaactc aataggtgca
     1081 atttgtttac aagcctttga taaagaagga aaaacttcta tacttgaatt taatcaaaga
     1141 gtattaggag tgaaactgga agatttaaaa aagataaaaa gaacaatagg tgtcgcttca
     1201 ggagatgaaa aaatagaggc aataaaggca tcattaaaag cgaaatttat aaatagttta
     1261 gcaattaatc acagtttagc acttaaatta ttagaagatt gtgactaata gaaagataga
     1321 taaaagttaa caggaggaaa tgtatatgtc aatatcaaaa gaaacattgc ttgaaatgta
     1381 taagagaatg aatcaggcta gaaaatttga agagaaagtt agttggtttt ttgcaagagg
     1441 tatggttcat ggaacaactc acttatctgt aggtcaagaa gcgtcaagtg tggcggctgt
     1501 aatggcttta gaaaaaggag atttagtatc attaactcat agaggacata gtcaatttat
     1561 aggaatggga atagacctaa ataaaatgat ggctgaactt atgggtaaag aaactggatt
     1621 ttgtaaggga aaaggaggct caatgcatat agcagatatt gagtcaggta acttaggagc
     1681 aaatggagtt gtaggtggag gtcttacaat agctccagga gctgcactta cacaacagta
     1741 taaaaaaaca ggcaaaatag ttttatgtag ttttggtgat ggagcatcaa atgaaggtac
     1801 ttttcatgaa ggaataaatt tatcttcaat atggaaattg ccaataatat tttactgtga
     1861 gaataattta tacggtatgt caacaagcat aaaacgtcat atgaatatag aaagtattgc
     1921 cactagagca gcttcttatg gaatagaagg aatatcaata gatggatata acccaataga
     1981 agtatacgaa acagtacaaa aagctgctga gaagtgtaga agaggagaag gacctgtatt
     2041 aattgaaagt agaacttata gatggttagg tcattcaaaa tcagatgcaa atgtatatag
     2101 aacaaaagaa gaaatagaat catggaaagc aaaagaccct attgaatttt taaaaaatta
     2161 tcttatagaa aataatctat ctaatgaaga tgaattagat aagattcaag aatttgcaaa
     2221 gcaatcaata gaagatgcag tagagtttgc tcaaaatagc ccaaatccaa aaatagagtc
     2281 tttattagag gatgtatatg cagactaaga atggtaaagg aggaaaataa aaatgagtac
     2341 aagggaatta acatatgcac aagcaataaa ggaagcaatg tcagaagaaa tgagaagaga
     2401 tgaaaatgta atatttatgg gagaagatat aggaatatat ggaggagcat ttggtgtatc
     2461 agttggtatg atagatgaat ttggaccaga aagagtaagg gatactccta tatcagaagc
     2521 agctatagca ggagcggcag caggtgcagc agcaactggc ttaagaccta ttatggaagt
     2581 aatgtttatg gactttgtaa caatttctat ggatgcaatt gtaaatcaag cggcaaaaat
     2641 gagatatatg tttggaggaa aagcacaagt tccaatggtt gttagatgtc caggaggttc
     2701 tggaactggt tcagcagagc aacattcaca aagtttagaa gcttggtttt gtcatgtacc
     2761 aggagtaaaa gtagttgcac cttctacacc agcagatgct aagggattgt tgaaagcagc
     2821 tataagagat aataatccag tcatatttgt tgagaataaa ttgttataca gaaaaaaagg
     2881 ttttgtgcca gaagatgatt atgtaattga aattggaaag gcagatatta aaagagaggg
     2941 tactgatgtt acagtaatta cttatggaag aatgttacaa agtgttgaag aagcagcaga
     3001 gactctatca aaagaaaata taaatgttga aattatagat ttaagaactt tatatccatt
     3061 agataaagag actatagtaa agagtgtatg taaaactggg agagttttaa tttgccatga
     3121 agcagctaag actggtgggc taggtggaga aatatcagcc ctgataacag aaagtgaatc
     3181 atttgattat ttagatgctc cagtaaaaag aatttgtgga aaagatgtgc caataccata
     3241 taatccagag ttagaaaaag cagttgtccc aagagttgat gaaatagaag aaacaataaa
     3301 atctttaatt gttagataag gagggataga tatggtggca aataaaataa aagccacacc
     3361 agcagctaga agtcaggcaa gaaaagacaa tataaaatta gataggctga ttggaagtgg
     3421 agaaaatgga aggattcatt tagtagatgt gttaaattat ttaaaagata ataaagcaaa
     3481 tactactcct ttagctagaa gaatagcaga agatttaaat atagatttag aaactattgt
     3541 aggtactgga tataatggaa agataagaaa gtgtgatgtg gaaaaattaa ctgcaaaaga
     3601 aactattgta tcaactaata catctaaatc tagtgagaaa aaagagctga aaattgaaaa
     3661 tgaaaattca agcatgttta acacagtaga aggaatattt gaaaagccaa atccaatgag
     3721 agctactgta gcaaaacgaa tgtcagaaag ttattttagt gctcctgtat ttacattcaa
     3781 tatagaagta gatgctacag aattgaaggt acttagagca aagttaatcg acacagtaaa
     3841 agaatcaact ggagtaaaat taactatgac agatttaata gtaatggcag tatctaaaat
     3901 acttccaaat catcaagcct taaattcagc atggacagat gagggtatat ttagatataa
     3961 agatgtaaat atagctattg cagttggttt agatgaaggc ttatatgttc cggtagtaaa
     4021 aaatgcaaac aaaaagtctt taaaagaaat agcaaaagaa agcaaagaac ttgcggaaaa
     4081 agtaaagaca gggaaactta tgccagcaga ccaagaaggt aatacattta caataagtaa
     4141 tgttggaatg tatggtataa ctacatttac acctataata aatatgcctt caagtgcaat
     4201 cttaggggta ggtgctacac aagataagtt tgttccagta aatggagagg ctaaaataaa
     4261 acctataatg aacttatcat taacatcaga ccatagagta atagatggaa cagtagctgc
     4321 aaaattctta aaagatttaa aagaattgtt agaaaatcct ttatcaatgc ttgtataggt
     4381 tttgatattt cgttactgat ataaatagtt tgaatagatt atattattat aattgtttca
     4441 gtaaatttaa agcttttata tattaagaat tatttttaac agtaactata tgtgaatatg
     4501 atttgcttaa ttaaaatata tgaaaataaa taggaggaga gcctatgtca gtagaagtaa
     4561 taatgccaaa agcaggagta gccatggaag aaggtactat agtatcttgg ctaaagcaag
     4621 aaggcgaaga agtaaagatt ggagaaccta tactagaaat aacaacagat aaagttaata
     4681 tggagataga gtcagaagga gaaggtacac ttgctgtaat catacataaa gaggaaggtg
     4741 aagtattacc agtatttaca gtaataggtg taatagcaga aaaaggggaa aatcaagagg
     4801 aagtaaaagc taaatattta tctggaaatg tttcaaaaga agatacagtt aaagagaatc
     4861 aaaatataga agttaaagaa gaaaaaataa acaaaaaaga gtgtaatcat gattatgatg
     4921 tagtggtaat aggaggagga cctgggggat atttatcagc attaaaagca gctcttttag
     4981 gtggaagagt tgctttagtt gaagaaaata tactaggagg tacttgctta tatagaggtt
     5041 gtatccctac aaagacttat ataaaaacag cagaaatatt agaagaaatt gaccaattat
     5101 caaagagagg tgtaaaagta actgtagata aagaacagga cattaagaag gccataaaat
     5161 ataaaaatag agtagttaaa aaacttacag ctggtgttgg cggattatta aaaagtagag
     5221 atgtagatgt gtttaatctt aaagctagtg ttaaagaaga gcataaagta attttatcag
     5281 atggaaaagt tttagataca gaaaatataa taattgctac aggttccaaa gtaaggatat
     5341 tacctataaa aggaatagaa tcaaatttaa taataactag tacagaagca ttagatttgg
     5401 aaacagtgcc agaagaatta gtaataattg gtggtggagt gattggttgt gaatttgcag
     5461 agatattcaa ttcaagaggt tctaaagtaa ctatagtaga aatggaagat agagtaatcc
     5521 caagaatgga taaagagtta agcgaatctt taaaatattc tcttagtaaa aaaggtataa
     5581 atgtattaac taaaaagaaa gtttctgaat ttaaagaaga aggaaataac atattagtgt
     5641 gcattgaagg tgaagagcca ataaaagcag atttatgtct atatgctata ggaagagaag
     5701 ctaatctttc tggcatagag gacttagata ttaaaataga taaaggttca atagttgtaa
     5761 atagtaaaat ggaaacaagt ataccaagca tatatgcagt aggtgatgtt actggaggtg
     5821 taatgttagc tcatgctgct tttaaaatgg gagaggtagc agcttcaaat gctctaggtg
     5881 tgaataaaga agttgactta ggtgcattac ctagttgtgt ttatacaatt ccagaagtag
     5941 catcagttgg tattacagaa gaggatgcta gaaaaaaata taatgtaaaa gttggtaagt
     6001 ttaattttgc tggaaatgga agagctttag cttctggtca agaacaagga tatgtgaaag
     6061 tagtagcaaa tgctaaatat ggagaaattt taggtataca tatgtttgga tgtggtgtag
     6121 cagaacttat aaatcatgca gcatcattta aagcattaga gatacctact gatgaagcaa
     6181 gtgagttgat atttggccat ccatgtactt cagaagcttt aatggaagct ttggcagatg
     6241 taaatggaga atgtttacac ttacctaaaa aataaatcta aacataaatt attaatttaa
     6301 gtctattatt aatattaatt tatatcataa aattttaaaa cacttactta gccatacccc
     6361 ttattatgca aataatttat ttgcataata aggggtattt tttatggaaa aatatagaat
     6421 atagaatcct tgtagttttt gttgtacaaa aaagatagta atataaaata acattatcat
     6481 tttagaatga tttcaattgt aattaggaaa agtgattttt ttgtttttaa aaaaagtatt
     6541 atttgtaaaa tataaaaaaa aatttcttaa tatttgaata aaagaatagc ttaatttaaa
     6601 tgttattact ttataaaaaa ataataagta caaaaaaaac aatatatggc atataagttg
     6661 ctatataaat aataaggagg gaacaattat aaatgaggca aagaataaaa aacttaattg
     6721 aaaatgaaga taagaaaaat cctttaacag atgaagcgat ttcaagtagg ttaaatattc
     6781 gaagagaaga tgttaccttt ttgagaaatg agttaaaaat agaagattca agacaaagaa
     6841 gaaaagttgt tttaattaaa gctataaaag atattttaaa agaagaaaaa actataaata
     6901 aaaatgatat aacaagaaga ttaaacagca ttgggtttac agtatcaaga tttacagtca
     6961 tacaatactt aaaagagatt caagattctg gtgacctaaa acttgataaa gagagtgatg
     7021 ataaaaaaga agaaataatc aataatagag aaaataaaga tatagcattt gataaactca
     7081 taggtagtag tggtagctta agtacaatta taaaactagc caaagcagca attctttatc
     7141 cacctcatgg tcttcatact attattatgg ggccaactgg tgttggaaag agtgagttag
     7201 cagagtgtat gtataaattt gcagtagaat catccagatt ccctaagaat gcaccattta
     7261 tagtttttaa tgcagcagat tattcagaga atccacagct tttgctatca cagttatttg
     7321 gacacgttaa aggtgctttt acaggagctg atgagaacaa agaaggatta gtatcaaagg
     7381 cagatggagg aatattgttt atagatgaaa tacatagatt acctcatgaa ggtcaagaaa
     7441 tactatttca actgattgat aaaggtatgg tcagaaagtt aggagaaaca agactaacac
     7501 ataaaataga tgtaatgatt atttcagcaa cttctgagcc aatagattct catttactta
     7561 atacatttaa aagacgtata cctgtaacaa ttgaaatacc agaattagtt gcaagaccat
     7621 taaatgagag gtttgacatt ataaataatt tcttcttaat agaagctcaa agaatgggag
     7681 caaacataca tataaagtca gatgtattaa aagctcttat gctttacgat tgtataggta
     7741 atgtaggtca acttagaagt gatattcaag tagcttgtgc taggggacta ttaaaccaac
     7801 ttacaaacaa actaaaagaa gtaaatataa gcatatcaga tgtacctgga tatgtaaaac
     7861 aaggtcttat aaaaatcaga aattgtagag gaaaaataga aaattatgta gatggggatt
     7921 taattataga ctcaaatctt caaggtgaga tagaaaagaa aaatgaagat atatatactt
     7981 ttccagatga aatatacaaa cttactgaaa gaagacatat tgatttattg gaacaaggtt
     8041 tagattttga tgttataaat agaatcattg gaggagaatt agaaagttgc attcaaaaat
     8101 acataaaaca agttaaaaag gtttctatta gtaacaatct tccagatata agtccaattg
     8161 ttggaaaata tatcgtagag ctaacttatg aagtaatcaa aatagcatca caatacctca
     8221 gttcactaga accgaatcta catttatgtt tagcagttca cctaaaagca gcttttgaga
     8281 gattaaaaga aggaaaagta atatcaaatg ctcatttgaa agaaataata ggaaattatc
     8341 cagtagaata ttctgtagca gaaaaggtag ctcaatattt aaaggaaaga tataacataa
     8401 acttaccaaa agaagaaata ggtataatag caatatattt taaaatgagt agtagaagag
     8461 atatgtctga acaaaaaaag ataggagtac ttcttatggc tcacggaaga gtagcatctt
     8521 ctgtatgtga ggttacaaat aaactattag gaattaatca cgcaaggtat ttagatatgc
     8581 catttgagaa gaagccggaa gaaatgattg aagaagctac tacaataata aaagatattg
     8641 atgaaggtag aggtgtgatt atattagtag atatgggttc actttcatat tttggagata
     8701 ttatttctgg aaagactgga attaatataa ggacaatatc tagatttgat acattgcttt
     8761 ctatagaggt tatacgaaga gcagttttac cagatttaac aatagacgag atagttgatg
     8821 aagttaaagg tatagagcca caagtaaaag aaaaggttag taaatgtaac aatacaaaag
     8881 tagattttag aaaacctata attttaacat tatgtattac tggattaggg ggagcttgta
     8941 agataaaaaa aatcttagag gaaaatatta aaagtataac tgaaaaggtt gatatagaac
     9001 ctataggtat gattgaatat ccaagtcttg atttagagat aaaacaaata agcaatgata
     9061 aaaatgtaat cgccatagtt ggaacaattg accctcagat agcaaatatc ccttttttta
     9121 gtctagtaga cataaatgat aaagcaaagt taaatcaaat aaataaccta attgatacaa
     9181 atcaaattgg tgaaacaaat aaaaaatatg aattatcaga tttaatacat aaagaagata
     9241 tatttgtgga tttacaggtt aaatcaaaag aagaagttct aaaatatatg agtaaaaaac
     9301 ttagagagaa aggttatgtg acaaaaaatt tctataaaaa agtagtggaa agagataact
     9361 tatttccaac tgaattatca gatgaaatag caatacctca cacagatgga atagatgtaa
     9421 ttaggccagc tataagtata gccatcttaa agaagcctat tgtgtggagt aaaagtaaag
     9481 taaaaataat acttttatta gctgtagacc aaaagtgttt taatccatta agcactctac
     9541 tctcatttat agaaacagat gaatttaaga agataaaaaa cattaaagat agagatttta
     9601 ttagggagat gatattagat ggagtcaatg aaaataatta gtagcaattt aatttttaaa
     9661 aatattgaag tgtctaacaa tgaagatgca ttaaagtttt tgggacaacg tttgtttgac
     9721 gaacagtatg taaaagaaag ctacatacaa gctgttgtag caagagaaaa aaagtatgct
     9781 acagggttac caacagaaat atacggagtg gcaataccac atacagatat agtacatgta
     9841 aatgagccag gaatagcaat tggaatactg aataaacctg taaaatttat aatgatgggt
     9901 acagacgata ctgaaattga tgtaaaagtt gtatttatgt tagcagtgaa agagccacaa
     9961 gaacaattgc aactattaga aaaacttatg actatatttc aagataagaa tatactaaat
    10021 aatattgtta acttaagtga agaaagtgtg agtgatttat taaactctaa attgaaaaat
    10081 taaacatgca aagtatgtta aaaaatataa aaagattatt aaagatttct aactatagat
    10141 atctttaaaa taaaataatt tcaatggggg tgactaatgt ggctaaaaag atattagttg
    10201 catgtggaac aggagtatgt acttcaacaa ttgctataaa caaattaaag aaagcacttc
    10261 aagacattgg taaattggat atggttaata ttacacaatg taaagtagta gaagttgcat
    10321 caaaagcacc tgattatgat ttaataattt gtactacaca agtatctagt agtataaaaa
    10381 caccagtgat aaatggatta ccttttttaa ctggtgtcgg aatggataaa ttaatcaatg
    10441 atgtattaga agagttagaa ttataattaa aaaaattaag gaacatggtc tctaaaatca
    10501 taagttttag agacttaaaa ttaaataatg gagggtcttt atatggagat attaaatttc
    10561 attgttggtc taggtgcaca agtaatgatg ccaataataa tatgtatttt tggtttgata
    10621 ttaggtacaa aactaggaaa atctttaaga gctggtttaa ctgtgggagt tgggtttata
    10681 ggtcttaaca ctattatagc tttgctaaca gataatttag gtccagctac acaacaaatg
    10741 gttaagaatt tagggctaag tttatcaata atagatgtag gatggccagc agcatcagct
    10801 atagcttttg catcaacagt aggggcttta attattccaa taggtcttgt ggtaaatata
    10861 gttatgctaa ttactaatac tactcaaact gttgatgtag acatatggga ttactggcat
    10921 tttgctttta caggagcttt agtggcaggg gctactcaaa gtgtaatgtg gggagtattt
    10981 gcagcagtag caaatatggt aattgtattg gtaatggcag atttaactgc tccaggtata
    11041 gaggaatatt taggtatgcc aggtatatct cttcctcatg gatttacaca ggcatttgtt
    11101 ccaattgcta tagtagtaaa taaattatta gacttaatac caggcataaa taagattgag
    11161 ataaatgctg atacattgca gaaaaaattt ggattatttg gagaaccttt aataatgggg
    11221 tctgtaatag gtgtaataat tggtattgct gcgaaatatg atataaaagg aatattacaa
    11281 ttaggtgtaa ctatgggagc tgtattaata ttaattccaa agatggcagc attattaatg
    11341 gaaggattat taccagtatc agaagctgca caggaattta ttgagaagag atttaaaaac
    11401 agaggaaaga tttatatagg cttagattca gcagtaggaa taggtcatcc tgttacatta
    11461 tcagttgcac tagtgttggt tcctttgact atacttatag cagctatatt gccaggaaat
    11521 aaagttttac catttgcaga tttagcagta attccatttg cacttgtatt aatcgtacca
    11581 attacaaagg gaaatgtgtt tagaacatta ataataggaa taattataat tacttctgga
    11641 ttattaatcg caactaactt agctccatta tttacccaaa tggctttaaa tgctagtttt
    11701 aaaatgcctg aaggagcaac tatgatttca agtatttgtg atggtgcgaa tccattaagt
    11761 tgggtatttg taaaagttat gaactataaa gttattggag gagtaatttt tggagttata
    11821 gctttaggaa tggctattta caataggaat agaataataa atgaaaataa aaagctatct
    11881 atagaagaat aaggttaaaa caattttatt aaggagtgta taggttatga aaaaatataa
    11941 atatgattct aaaaggtact tactcagtgt tacaatacca ggatttttta tgattttaat
    12001 attgatttat gcactgttta ataactatac aaattttaat ttgaatatat atagtttgtt
    12061 aataattgtc tgtacttatg gtatattcaa tacttttata tcatcaagta atcctaaaaa
    12121 aattattatt gatagcaaat gtatacattt tacctcattt atgactacac ataagtatga
    12181 aattaaagat atagaaaaga taagaataag agaattttat aataaaaaaa tttatctaaa
    12241 aattaatgat ggaaaccttt ttaaaggtag gtattggata agaacaaata tgtttaataa
    12301 tagtatagca ttatatagct atttcattga gttggaagag tgcttatatc cagattcact
    12361 aaaatttaga aataaaaaat ttgagaataa gaacatataa aaataaaaaa ttgaattgaa
    12421 gcctagctta atcttttttg ataagatata agtttgaact ttatactaaa tttttagaat
    12481 tttcaatcaa gacaataaca taatagctaa gcaattagaa cacaattaag atgtataagg
    12541 catgtatatg atgaatggga ggatttaatt aatgttatta gagaaattaa gaaaagaagt
    12601 attacaagca tctttagacc ttttaaacta taatttagtg acattaacag gaggaaatgt
    12661 aagtgggaga gatgaacaaa ctggctatat agcaatcacc ccaagtggaa tggattatag
    12721 aaatcttaca ccaagtgata ttgttatagt agatgtagat ggaaatatta tagatggaaa
    12781 atggaaagca tcagtggatt tatcagacca cttgtatata tataagcata gagaagacat
    12841 aaatagtata attcatacac attctacata ctcaagttgt tttgctattt taaatgaacc
    12901 tatagaatgc gcaagtacaa ctcttgcaaa tgaagtaggg ggttcagtac cagttgcaaa
    12961 attttcacca ccaacatcta aaaagatggg aaaatgtgtt attgaagcaa ttggtgacaa
    13021 aagagcatgt cttttagcaa atcatggcgt aattgcagtt ggtccatctg taggacatgc
    13081 tcttacagcc gctgtaatgt tagaagattc agcaaaagta tattacttag caaaatcaat
    13141 tggcacacca gtgctactgc ctgatgaaga aattcaaaga gcgagagatg tattttttaa
    13201 tgtctatggt caggataaat aaattagata aaattttaaa agtaatatga aaatatagat
    13261 attcaatgat attaaggaga tttataatga tataataagt ttaatagtta agaagtaaaa
    13321 tatgaaatgt aatagtatat aaattcattt aagactaaaa agaataagat aaaataaaaa
    13381 ggagtctgta tttctatgta ttacttatta aacaaaagca caaatcctta ctttaatttg
    13441 gcattagaag aatatctatt tttaaatgat aaatataatg atgatattat aatcatatgg
    13501 agaaatgaag aatccatttt tataggtaaa aatcaaaatc catatcagga agtatatcat
    13561 gatgttattg aaaagggaga aattcctatt ttaagaagaa tatctggagg aggaactgtt
    13621 tatcatgact taggtaatat caatatgtca ttcatacaaa aagataggca acttcatgag
    13681 atagattttt tggagcatac taagtttatg caaaagatgc tttcaacctt aggactagat
    13741 gtatctataa ctgaaagaaa agatttattt ttaaatggga aaaaaatttc aggctctgca
    13801 cagtctataa aaagaaaaaa ttctctttat catggaactc ttctttacga ttcagattta
    13861 aataaattaa ctaaatattt aaattcaaat aaagcaactg aatcaaatgc aactaaatct
    13921 gtttcaagta aagttacaaa tattaagttt ttactcgaaa aagatataaa ttacttttta
    13981 gattattgtg ttgaatattt aaaatctaat ataaagaata taaaagaact tgaattggat
    14041 gaagaagata ttaaaagtgt atacaatata ggagaagata aatataaaaa gatagagtgg
    14101 acatatggta aaacgccgaa atttcaagta gtgctaccat ttaataatga aagtaaaata
    14161 aatatacatg ttaacagatg gaaagtatca aaatttttta tttcttatga tgataatgta
    14221 attaatatgg acaagttgtt agatttgaat ttctttaaag aagaaataaa agagagtatt
    14281 ttagaaaatt atccacatta catagattta atcaatttaa tattttaata taaaacctct
    14341 aatttgataa aattgtctta tatcaaacta ga
//
LOCUS       DAEMHW010000097        14353 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEMHW010000097 DAEMHW010000000
VERSION     DAEMHW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.097"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..810)
                     /locus_tag="KPK67_002347"
     CDS             complement(<1..810)
                     /locus_tag="KPK67_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBG2459304.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEIN"
     gene            complement(945..1703)
                     /locus_tag="KPK67_002348"
     CDS             complement(945..1703)
                     /locus_tag="KPK67_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG2459305.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     ASIGVGIVEMLLSALLLTGLGSSIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(1705..2421)
                     /locus_tag="KPK67_002349"
     CDS             complement(1705..2421)
                     /locus_tag="KPK67_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG2459306.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLICSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLINMFFT
                     IVGVAVFSTTEMWYIFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(2423..3130)
                     /locus_tag="KPK67_002350"
     CDS             complement(2423..3130)
                     /locus_tag="KPK67_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG2459307.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGE"
     gene            complement(3246..3782)
                     /locus_tag="KPK67_002351"
     CDS             complement(3246..3782)
                     /locus_tag="KPK67_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459308.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINSIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(3884..4954)
                     /locus_tag="KPK67_002352"
     CDS             complement(3884..4954)
                     /locus_tag="KPK67_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG2459309.1"
                     /translation="MNKKLAIFAAVLLVIGVIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     YISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(4947..6005)
                     /locus_tag="KPK67_002353"
     CDS             complement(4947..6005)
                     /locus_tag="KPK67_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBG2459310.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(5992..6324)
                     /locus_tag="KPK67_002354"
     CDS             complement(5992..6324)
                     /locus_tag="KPK67_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG2459311.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            6523..7644
                     /locus_tag="KPK67_002355"
     CDS             6523..7644
                     /locus_tag="KPK67_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBG2459312.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIITGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(7772..9670)
                     /locus_tag="KPK67_002356"
     CDS             complement(7772..9670)
                     /locus_tag="KPK67_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBG2459313.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEKIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            9985..10761
                     /locus_tag="KPK67_002357"
     CDS             9985..10761
                     /locus_tag="KPK67_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG2459314.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKISYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(10853..11605)
                     /gene="xth"
                     /locus_tag="KPK67_002358"
     CDS             complement(10853..11605)
                     /gene="xth"
                     /locus_tag="KPK67_002358"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBG2459315.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSSTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(11766..12716)
                     /gene="galT"
                     /locus_tag="KPK67_002359"
     CDS             complement(11766..12716)
                     /gene="galT"
                     /locus_tag="KPK67_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBG2459316.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            13101..14297
                     /locus_tag="KPK67_002360"
     CDS             13101..14297
                     /locus_tag="KPK67_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2459317.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
BASE COUNT         4918 a         2342 c         1590 g         5503 t
ORIGIN      
        1 atttatttca attaataagt cagtatttcc atgtataatt gttagtggtg tttttatatc
       61 atgtgccagt gcagatatct gttcctttct attttcttct agcatccatt gtttttctaa
      121 agaatctttt aacgccaact tcattttatc catagaaaaa aggacattat taatttcata
      181 aattttacta gattctacaa caaaatctaa atcctgttgt tctatttttt ctgtagtatt
      241 ttttaaaagc tccatttcaa tattaaattt cttgccaaat acttttgata atataacaat
      301 ttcaataaaa aatattatac taaaaataat catacccaat gtctctggtt ttggtaaata
      361 tgctcttaaa attggagaac tatactctga tactattttg tattttatta taaaaacttc
      421 atctttctta aaaaacttta tataactttc tgaatatgca tgcctttctt caatatctct
      481 cattaaattc catacttctt ttgattcttt tttattaaag gtccctgaaa ttaaattccc
      541 atccaaagta taaaccccat actcatatat acttggaatt atatcttcag ttactttttc
      601 acttgaaata atctgttctt tatatttatt aaattgtttt tctgcataat tagctggtaa
      661 aattacacct gatgataata atacagaaaa tgataacatt aaaaggagta caattgaaat
      721 tgtcataata aagaataaag ataaatattt tataaaaata gttcttaact tttcaacttt
      781 tttgttgtta gattttttac ctccaagcat ttgtaccctg tcctccaata gtttaattaa
      841 ttcataatta atacctagcc agttttttat ttgattattt tatatctttt tattgctaag
      901 ttatatttta acataataat ataaataaac agtcaatttt tactctattc ttctgatttt
      961 ttaccttccc atttagaaaa ccaaatacat aaaattacaa atgctaaaat tgttacaaaa
     1021 ccacaaatta aacctgttga ttgatatgct tttattgatt caacctttat atattcaacc
     1081 gttttagaac ttgaaaaaga cgaccaaata gttataaatc taacacccca tgcacaagga
     1141 aaatactgcc aaatactaga acccaatcca gttaataata atgctgaaag taacatttca
     1201 actataccta ctccaattga tgctcctttt ccgaacctta aacttaagaa taaatgtaat
     1261 atgtattcaa ataaattaca cccaattaaa attagaattg aaatcatata aaagcttgat
     1321 gtaaagtaag ccttagatat accaaattta aaacctaata cagacactaa tgttgctaca
     1381 aaaccaagac atactaagat tatatattta cttataagag taagatattt tggattagat
     1441 gttgttaaca tatgtttata attccctgca taatattctt gttcaacaaa cattgaacac
     1501 acaacacttg acaataaagg aaaagctatt gctataactt gtaaatatgc tactatcttt
     1561 agtatattat cagctcctga aaccttataa taccataaaa acaatcccat ccccattaaa
     1621 gcaatgcaaa tgtgcattag tattataaaa tttctcttta attttattaa atcagaacaa
     1681 aatattctta ttaactgtct catattactt cacctctcta ttattgaacc aaacccttgt
     1741 agctaaagca aataatacaa ataaaacaat agatattaat actcctggca aaataacagc
     1801 attatttaaa aattcacttc cctctggtac aggaagacca tttggcagaa tatgcaatac
     1861 aacagccatt aatcttgaag gaatcgaata tggaaatata taccacattt ctgtagttga
     1921 gaaaacagca actccaacaa tagtaaagaa catatttatt agaactgtaa caaaaatacc
     1981 aaattttgaa gctaaaaaca tacaaagagg aatctgccac ataaatagta aaataattaa
     2041 tatagttgca atgatatttt gacctattga aatagtttct ttatagatta tcccaacaaa
     2101 aactacacct attgcagaaa taattgatgc tataaaacag caaataatac agtaaatcat
     2161 ttttccagtc caaacatctt tcatttcaat tggcaatggt aaaataccac gatatttact
     2221 tttttcatct tttaatacta ttaaagaaca tattaatgtt aatgctcctg gaagaaatgt
     2281 cgtataccac cagttaaaag cagcattttg ccctccacct agtccacaca aaagtataca
     2341 aaacactgga actatccata tcaacttcat tccaaaagtt cctttcatct tttgaaattc
     2401 cactctaata aatctttcca tattactccc ctctctttct attttcagct acaactttca
     2461 taaaaagttc ttctaagttt tgttcttttg atacttctcc ttgataaccc attactccat
     2521 tagaaattat tcctatttca tctacaactt gttcaacttc agacaagata tggcttgata
     2581 aaataactgt aattccttgt tttggaaaag aacgaatcaa ttctctaagt tcttcaatac
     2641 caatagggtc aagaccattt gtaggctcat ctaagattaa aagttttggg tgatttaata
     2701 atgcaatagc aataccaagg cgttgcttca tgcctagaga gaattgacct gaacgctttt
     2761 taaaggtatc tcttaaatct acagtctcta aaacttcttc aatgcgagag tcaggaagac
     2821 caagtaaagt agtatggact tttaaatttt ctcgtgctgt taaattctca tatatagcag
     2881 gagactcaat aagagaacca atatttgata aatcctttct agtccatcta tgtccctcaa
     2941 acattatttt tcctgaagtt ggatttaaaa tcccagttat catttttaat aaagtagatt
     3001 tcccagctcc atttggtcct aataatccat atatagaatt agatttaatt gctagtgaaa
     3061 tgttatcaac tgccatctgc tccttaaatt ttttagaaac tccttttacc tgcaaaatat
     3121 aattactcat aattttcatc ctttcttttg tcaaacttat ttacaagaat cattctaaat
     3181 cataaatata aggattttat aagaattaaa aaacaaaaaa agatgtgtta aaaacacatc
     3241 tttttttatc ttaattctat taattttcca ttgtagtata tctctacttc ttcaatccaa
     3301 atgctattat cttcttcttg gatgacattt ttaataatac tatttatagt atatattata
     3361 ttttgttcat tcaaataatc atctttccaa gaaactctac tttttttatc tggttctatc
     3421 aagtttattt taacaagatt atctttttcc ttaacttcta cttccatgtc taaaccatta
     3481 aaatactcat ttgataatgt atctaagatt aatcgcagtt tatccttaag tgaactttct
     3541 tcatcaatct gtaagtactt tactattttc tcatcctttg aaattatctc tataggtatt
     3601 ttagtagttt ttttagaatt aatcatttta ctagttgttt cttcttttgc atcttcatcc
     3661 ttctttacaa ctgctgattc tttagaatca actagctcaa ctgcactgat gctataatcc
     3721 atacatgccg tactcaataa agtagtagca attattaaaa aacttactgc tattttattc
     3781 ataagtatcc ctctcctaat tctctttcga tacttattat accatagtat agattatatt
     3841 tttatacttt taaatcaatt tataaattat tatagtaaat tatttacaat tctaagctat
     3901 ttgaatttat ttttatagta tattctttct ctaaattcga cagattttta ttcaaaagcc
     3961 cttttatttc tctcgtattt ctatacttgt tgctatgttc ttcactatgt tcataatcat
     4021 atgaatcatt gtcatattct tcatcattat taaattcttc aattgcttct tttgacttta
     4081 tatcacaatt aaatttatac ttatttactg gtatatcaag tcttacctta gaatttggaa
     4141 cttctatgtc caggaactta gcaattggca tatcagagta caagtaaaat tcactttcat
     4201 aatttgaatt tctacctatt ttctctttta tatttacatt ttcaggtata tactcttcta
     4261 tattattgat aaatacatct tcattctctg aagatatata caatgactct gattctatgt
     4321 ttacattttt aacgcctaaa attgattttg ttgacaagtg aagattgtta ttagatatta
     4381 agtttaggtt ttctaatcta tttactttgt tctctgttat agctctacta aaacctccat
     4441 aagaagtttt atatgttata gtatttgaag ctacattatc gtatacaaat aagtgtgagc
     4501 ttatacttga tgcatttatg tctacattat ttggtacata tattataatt tctctataat
     4561 catgtgaaaa catactattt atagatgtat ttaataattg gtcgaaattt ttaatctttt
     4621 taattttatt ttcatatttt ctttcacctt ttacaactaa agtcttatct tttaaagtta
     4681 cattgtattt gtaaaactca ttatttcctc ttgttgttac acgcacatct tcacttgaac
     4741 ttttctttat tgtaacgttt atattgtcaa cagctatatc taacttgtca atatttactt
     4801 tttcttgata aagtttattt tcttttttaa attcagcttc cttttctaaa ccaaagttta
     4861 ttaggcttgg cataactaaa atccctgacc atgttgtccc tattacacct attaccaata
     4921 aaactgcagc aaatatagca agttttttat tcatgtttat cttctccttt cttattttct
     4981 atatcttcaa gatccttata tatcgcctca aaatttatac tatcatcatt atattcatca
     5041 gtttctttta tgctttcttc ttctctattg tagtaatcat tattatatga attttcatta
     5101 atgtctttat tcaaatcttc atcatttaaa gtagcttttt ttcttttttc catcttttca
     5161 tttgccttta tataaatatt ttttctcttt atccaatgta tataattatt tagtaattgt
     5221 ttacctagtt ttatggcaaa taataaaatt tgccatgcaa gtatctcaag ccctacaaat
     5281 gctatggttg cgaaaataca aaacatcata acatctggaa ccattgctat aaatttaatc
     5341 acaaatggaa ttaatataac aaaagcaatt aatgagcata ctaatgcaat agtagcagat
     5401 acaatagcac atacaaacgc aaatgctgga acaattagaa ataaagataa cactactaat
     5461 aaccccttta taacttgtct ggataatcct cctacacctt cttctatact tggtgtaagt
     5521 ttatcattta tattttcttt tgatttattg tacttttcct tagcttcatc tttcaagata
     5581 gagtaatcct tttttacatt tgatttaaat ctactaaatt taccatttaa tatgtctgta
     5641 ttcattattt ttccatctat attttctttt gatttactat acttttcttt tgcttcatct
     5701 tttaagatag agtaaccttt tttcatatct gatttaaatt tactaaactt atcattcaaa
     5761 acatctgtat ttattatttt ttcctcttca atattttttt ctttagtttg taagataagt
     5821 tcttttgcta tagattttgg tgagccaagt gatgctatta tctccatatc actctttccc
     5881 tctataagac catctacgaa atactcttca taatctctca atatatcatt aacttcgctc
     5941 tcagaaaaat ctttttttag ataatccttt aaaatttcaa gaaactcagt tttattcaag
     6001 ttcattacct ccctcatctt cacttattaa aatctcaaca gcctttacaa attctctcca
     6061 ctcttccatc agattggata aattctcttt tcctagtgaa gttattctat agtattttct
     6121 agcaggacct tcattagatt ctagtatata tgtttcgaaa tacaaatctt ttgtaagtct
     6181 tcttagaata ggatatattg ttccttcatt tacatctatt actttagaga tgttatgtac
     6241 tatctcatat ccatacatat ctttcttaga tatgagagca agtacacaaa tttctaaaac
     6301 accctttcta aattgtgtgt tcatatactc catcctttct ttaaatacac agtattgtgt
     6361 tttacaaggt actatgttaa aacttcttat atcctcataa taacacagta ctatgttctg
     6421 cacaatagtt atttttaaat attcttgttg attaaatcct tctataaatg tataatactc
     6481 taatcagata tattttatga aagtggtgat aaattggcta aaatgaaaga tgataaaaca
     6541 atattaatcc taactgctca atttggtgct ggacatataa gtgctgcaaa agcagtcaaa
     6601 gaatgtatta ttgaaaaata tagtaactat aatgttgtta tacaaaactt tataaatgca
     6661 agtattccca tgatgaataa gcctatggta aaactttatg aaaacaatac aaaatatact
     6721 cctggattat ataattacta ttattacttt aagaaatcgt ttgattcaag acatgattta
     6781 tctcataaat tatatacacc taaactttct gagtatattg ctgatataaa tccagatttg
     6841 attatctcta catttccact agctgctgct tgtgtaaata actttaagat aaaaaatcct
     6901 gatataaata tacctacact tacagttata acagatgttg tagatagtat ggaatgggtt
     6961 tttgaaaata cagatttgta ttttgttcct tctcctgaaa taaaaaatag attttttcag
     7021 aaaggaataa atccagactc cataaaggtt actggagttc ctgttgataa gagatttcaa
     7081 attgaaagta aagaactatg ttgtgataaa tatagactat tacttctagg tggaggaaga
     7141 ggtctatttg atatagatga ggattttatg cattggatag atgaatttat tgaagaacat
     7201 agtaattcta tagaaattac aataattact ggtaaaaaca aaaaattata tgacaatcta
     7261 actcataaaa aacctctaaa aaatattaaa gtacttggat ttgtaaacga catgtataat
     7321 ctaataagag aatgtgacct aatgctaact aagcctggag gagcaactat atttgaagct
     7381 attcaatcac aaactccagt actcgtaaaa atgccaaaag ttggacaaga aattgaaaac
     7441 gctaaattta taatagataa gggtcttggc atgatttata gtgatgacct agaccttaaa
     7501 aacatatttt ataaattagt atcaaatgaa tttgattcta taataaactt tatgaaaaaa
     7561 aatttagaag aatttaaaac agtcattcat ccagagaaaa ttgctgatta tatatcagaa
     7621 ttaatagata atcattacag ttaaattgta ctattaaaaa atttcaaaca taaaattaaa
     7681 agcaatcaag tatgctttta aacttgagaa tttactcata gtgtttaata atacttgatt
     7741 gcctttatca tatcaaaatc aaattgttat attagtatat atttttcttg tactgcatat
     7801 acagttgttg tatttcttcc atacttctct ttaattctat ttgtaaatct gaagcagtct
     7861 ggtaatcctt attttctata gcttctctca ctctagtgaa tagtgattct ctagttaaag
     7921 tatccttcat aatattgaac tttaaatccg atattgagtt ccaaactacc tcatcaagcg
     7981 catccatatt ttcttttgtg tgtatcttga caatacttct tattttttga atcccagcct
     8041 ctattattct agtttttaat ttcttttgcc acttatctat agctccaatc ttaaatgatt
     8101 ctattatact atcagtaaat acatcacctt gttttaaaga ttttaatttt gaagcatata
     8161 tttctaagtt tttcatattt tcataaactg tagctggtgg tattccaaat cttgcatttc
     8221 tttcttccaa actataatgt tcaaatacat cattttcatc tctatatgct ctatcttttt
     8281 ctaaatagaa actttcttca cctacatttt tagataactc tttttcaagt tcttttgtac
     8341 taagaccact cttagctgct gcttttatac catccaacat tgtttgataa catcctgcta
     8401 ttactaaata cgtgttagat agagggcttg gtgaccttaa ttcaaatctt acagtctttg
     8461 gatttttcat atctcttatt aatccaacaa gcactgacct atttcttgat ggaatctcat
     8521 aactatgccc taaagatgta actatacata caggagcttc aaatccagga actaatctat
     8581 taaatccatc attagaagct gttacaattg ggtttaatac ttcataattg taaagaagtc
     8641 ccataagagc cccatatcct agttcactta gataatcttc ttttaaatct tttggagcaa
     8701 atagattttt tatacttcca tcctttaact tcgcacttac tcctacatgt gtatgtccac
     8761 cacttcctgc tactccatga attggttttg ctttaaatgt aacttctagt ccatgtgatg
     8821 taaatacatc ctctataaca tctcttacta ataattcatt gtctgctgct tgtaatggtg
     8881 tagaaaactt ccaagaaacc tctaattgct ccatagcatg gtttgttctt ccatctatac
     8941 taatagaact ttgtattcct ccgacctctt tgtgagccat ttcagggttt acacctagct
     9001 tttgaagtat aattaaactt ttctctaaac aagttcttat aataccatga gttcttttcc
     9061 agtattgctc ctttaggctt tgtgatacat ataatttctc taagtcagct ttatcctcag
     9121 gagtattaac ccagaactca agttctgtag ctgctgttaa cattattttc tctatatcat
     9181 ctactgaatc tattcctatg ttatttatta catgtggata ttctctaaat atttcataca
     9241 ttgacttttt aaagtactta tctgcttttt gaagtactcc tctagagcat acttttttat
     9301 tatcatgtat taaaaatgca ggtatcttta atgttccaac aggtagacct acttcttcgt
     9361 ctatatattc catattgtaa tctacatacc aatgacaact tttatctggc attaaatcta
     9421 ctttcgcatt atttaatgta gctatattat aaagctcaac acttgaacca tcagtctgta
     9481 tagctgattc caaaaattta tctatatcct ccaaaaataa ttctacagga atcttttcat
     9541 ctgttgcatt tcctcctaaa tcaaccccca ttaaggaaac aaatcttata tttttattgc
     9601 tttttaggat ttctcttaat tcttcattag tgtgtttatc tttctctatt acgtataata
     9661 gactatccat ttttccactc tcccataatt tttatgtata ttttattttg ttaaatacaa
     9721 gataaatttc attttcctta catttatgaa tttttcttgc aaattattcg tgttttattc
     9781 gtgtttttat ttatattatt atatatcaag tataaaaagt tcgcaatatt ttttattgga
     9841 aaaaatatta tacatacaat agtttaacat ttttacaaat atggggtata ctaaaataaa
     9901 agtattttta tttgaaaatt taattttatt tataatataa tctatagtaa aagaaataat
     9961 aaaaaaattt aggagataaa ctttatggaa tacataattt tcgatttgga atttaaccaa
    10021 ggatttgata aaaaactcaa taaaactgta tccaatgaaa agtgtccttt tgaaattata
    10081 caaataggag caattaaatt ggattctaaa ttcaatataa tagatacgtt taatagctat
    10141 gtaaaaccta caatttataa agacatacat ccctttatta gtcgtatgac taatattaaa
    10201 aattctgatt ttaatgattc gcctactttc cctgaggtct acaacaattt tataaaattt
    10261 atatcatcac aagaccctat attatgtgtt tggggagctg gagatttaaa ggaattatac
    10321 aggaatatca actatcacaa gctaccatca aattccttac ctaaatctta tattaatata
    10381 caacaacatg catctaaata tttcaataat ccagcaggaa aaagcattgg cctacaaaat
    10441 gctatttcta tattggaact tgatgaaaaa atatcttatc acaatgcttt aaatgatgca
    10501 tattatactg ctaaagtttt tattaagata tacaatccaa gtatagtccc agatatatat
    10561 ctttatacga gtataaaacc taaaactata agatattcta ataaaaaaag agttgattat
    10621 gataagttat ttgatgaatt taggaaaatt ttaaatcgtg aattaactaa agacgagaaa
    10681 aaaatcatta acttagctta taatatgggg aaaacaaatc aatttacatt agaaaatgtt
    10741 aagcaaagaa aaaacaaata gagtttattt agataaaaaa taggcatgta gattaaaaaa
    10801 ctagttaact ttaaatttat atctacagcc caatttttat cataattaat ttttatttta
    10861 tctctaattc aactggacaa tggtctgaac ctaatatttc agtgtgtata tccgcactta
    10921 ctaatctatc ttctaatttt ttggatgcac aaaaataatc tattctccac cctgcattat
    10981 tttttcttgc attaaatcta tatgaccacc aactataaac accttcttta tcaggattaa
    11041 aatatctata tgtgtctata aacccactgt ctaataattt agaaaacttc tctctttctt
    11101 ctttagtaaa acctgcattt ttcatattat ttttaggatt ttttaaatct atttctttat
    11161 gtgcaacgtt catatctcca cacacaatta caggcttatg attatctagt ttggttaaat
    11221 aatctataaa gtcgtcttcc catctagttc tatattctag tctctttaat tcagattgag
    11281 aatttggcgt atatactgta acaaagtaaa aatcttcaaa ctctagagtt atcactctac
    11341 cctctttgtc atgttcttct atatttattc catacattac ttttaaaggc tctttttttg
    11401 taaatatagc agtacttgaa taaccctttc tctcagcata attccaatat tgaaaatatc
    11461 ctggtaaatc taactctatt tgcccttctt gcaatttagt ttcttgtaaa caaaatatat
    11521 ctgcatctac ttctttgaaa aagtctaaaa atcctttccc aacacaagct cttattccat
    11581 taacattcca tgatataaat ttcatactta tactcctcat ctaaacattg acacactccc
    11641 catagctaaa gatgggggct tttcaacaat tttattgtaa attcttaatt cacttttatt
    11701 ttaacaatgc tatactataa tttcatctaa aattactatt tacttaatac ctaaaaatat
    11761 atgttttaat caatttttaa cttttttgtt aaatcttcag ggctcattga agaaacatac
    11821 atatttgttc caagttcaaa tcctccaaaa ttatattttc ttgggattat gtgcatatga
    11881 acattaaaat aagattttgt ttcaatcttt gttggatgag tgtgaataca taagttaaat
    11941 ggcatatatc cattgatatt gtataacttc ttaaacaact tttcaaatat tgtagaaagc
    12001 tcctgtatat tattatcatt taactcacca aactctattt tatctttaaa tattattctt
    12061 acttctccac tatacttggt tgcatatggt ataagtacca aaaaagcttc tccattataa
    12121 acaactcttc tatctaaact tatttcattt tctattaaat catcatacaa agagctttta
    12181 ttgctgtcat aatactcctt tgaaaccaac agttcattcg taatttcagg tggaataaaa
    12241 gacatagaca atatttgagc atgtggatgc atcaaagatg ctcctgcctt tctcaagaag
    12301 tttttgaaga tactaacata ctctacttta tcatctttcg acaaatctct aaatctattt
    12361 ctatacataa aaaaaacatc ttcaaattct ttttgactca tgttataaaa actacgatta
    12421 tgtttatcgc tctctatcat tacttcatga acaccatata catctcttgc catatcatca
    12481 attataggaa atttattgta gacagatttt acaagccatc cttcttcact ttctatttca
    12541 aataatgcat cagttgcatg agcttcattt cctttgcaaa atgggcattc ttcatcatat
    12601 tcattagttt cttcatcttc atttgggata tctgctgtat ctaaaggtct ttttaaccta
    12661 tctgtagcaa atataacaac atcattggta attggatcta ttcttaattc tttcataatt
    12721 aacacctctt agttaatagc ctttaatata attatatgat atttatcgta tctgttaaat
    12781 aaatttataa tattttttat aattttgcat atttattatt atatattttg tctatagctg
    12841 aaagattata actaaaaaat cactatcagt atatcagaat attatcttac tataaattaa
    12901 tttatgaata cataatttag tggcataaac atgggtattt tactataatt tgttaaaagt
    12961 ttattttaaa gctatattaa ttttttttga tgatttttac ccataagtat gttattatta
    13021 tcttgtaaag atgtcttgta ttcaaacata caatttacat aatacaatat acgtatttaa
    13081 attaaatagg gggtattaaa atgaattatt caaacagagt tagtgcaatg caagcctctc
    13141 caatcagaaa acttgttcca tttgcacaag cagcaaaaga taaaggaatc aaagtatatc
    13201 acttaaacat aggacaacca gacataaaaa ctcctaaagg attttttgat gctgttaaaa
    13261 actttgatag cgaagtctta gaatatgcta catcagaagg tattccagaa ttattagaag
    13321 cacttcaaaa ttactataaa acttacgata tgaattttga aaaagatgaa ttattagtaa
    13381 ctaatggtgg tagtgaagct ttacttttca ctatgatggc agtttgtgac ccaggagata
    13441 atctattagt tccagaacca ttctatacaa actacaatgg atttggtcaa tctgtaaacg
    13501 tagaagttaa tgctgttaca acaaaggcag aaaacggttt ccaccttcca tctaaagaag
    13561 aaatattgtc taaagtagat gataaaacta aggctataat tctttctaac ccaggaaatc
    13621 caactggtgc aatttataca aaagaagaac ttaatatcct agctgaaata gctaaagaaa
    13681 aagatttatg gataatagca gacgaagttt atagagaatt tgtttatgat gggcttgagt
    13741 atacaagctg tggtaactta gagggtgtac aggatagagt tataataata gacagtgttt
    13801 ctaagagata cagtgcttgt ggtgctagaa ttggttctat cgcttgcaaa aataaaggtc
    13861 ttatcgctca aatacttaaa ttatgtcaag gaagactttg tgttcctaca ttagagcaaa
    13921 taggagctgt tgagttatac aaaactcctg tttcttactt caaagaagtt aatgaagagt
    13981 acaaaaaaag aagagatgta ttatacaatg aattaatgaa ggttgaaggt gttatatgta
    14041 aaaaaccaac tggtgctttc tatatagttg ctaaattacc agttgaaaat gcagaagatt
    14101 tcacaatttg gatgttaaaa gaatttaaca aagataatga aacagttatg gtttgccctg
    14161 ctgaaggatt ctatgctact ccaggtttag gacgtgatga aataagatta gcttacatct
    14221 taaatgaaaa agaccttcat agagctgcta cattattaaa agaaggttta gagcaatatg
    14281 tagctttaca aaaataattt tttaaactta atttatataa atttaatgat ataaaatgtc
    14341 cccttttcaa ata
//
LOCUS       DAEMHW010000098        14194 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEMHW010000098 DAEMHW010000000
VERSION     DAEMHW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.098"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..495)
                     /locus_tag="KPK67_002361"
     CDS             complement(<1..495)
                     /locus_tag="KPK67_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2459318.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVI"
     gene            complement(482..1249)
                     /locus_tag="KPK67_002362"
     CDS             complement(482..1249)
                     /locus_tag="KPK67_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459319.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPDEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            1537..2211
                     /locus_tag="KPK67_002363"
     CDS             1537..2211
                     /locus_tag="KPK67_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2459320.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            2235..3239
                     /locus_tag="KPK67_002364"
     CDS             2235..3239
                     /locus_tag="KPK67_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2459321.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            3351..4580
                     /locus_tag="KPK67_002365"
     CDS             3351..4580
                     /locus_tag="KPK67_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG2459322.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            4588..5262
                     /locus_tag="KPK67_002366"
     CDS             4588..5262
                     /locus_tag="KPK67_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459323.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            5265..6497
                     /locus_tag="KPK67_002367"
     CDS             5265..6497
                     /locus_tag="KPK67_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2459324.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(6783..8048)
                     /locus_tag="KPK67_002368"
     CDS             complement(6783..8048)
                     /locus_tag="KPK67_002368"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBG2459325.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(8101..9249)
                     /locus_tag="KPK67_002369"
     CDS             complement(8101..9249)
                     /locus_tag="KPK67_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG2459326.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(9349..9894)
                     /locus_tag="KPK67_002370"
     CDS             complement(9349..9894)
                     /locus_tag="KPK67_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG2459327.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(10117..11061)
                     /locus_tag="KPK67_002371"
     CDS             complement(10117..11061)
                     /locus_tag="KPK67_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG2459328.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            11447..12328
                     /locus_tag="KPK67_002372"
     CDS             11447..12328
                     /locus_tag="KPK67_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG2459329.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPGRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(12389..13597)
                     /locus_tag="KPK67_002373"
     CDS             complement(12389..13597)
                     /locus_tag="KPK67_002373"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBG2459330.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     RIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(13670..13922)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         4971 a         2236 c         1790 g         5197 t
ORIGIN      
        1 aataactata aatattatta aaaataccgc tagtgtctga actaaggcct ttatacttaa
       61 gttcatattt actactatca tttcattcat taattttaca agcatcatag tcataagctt
      121 tgaaaataac agtcctaaaa atatacctat tagtattgca actataccca tcaaaaaagt
      181 ttcatataaa aatatttttc ctatctgatt tttcctcatt ccaagtaaac tataagttcc
      241 tacttctcgc ctacgtctat tgaaaaaaaa tgagtttgaa taatatataa ataaaaatac
      301 aaataccact attacaattg aacttgtatt aatactcatc ctagttttct ctccaagtga
      361 actcaacgct tcattatact ctatagattt aaaactaaaa aatacaaata cactaaatac
      421 aattgaaata aagtacatta aataattaaa aaaactattc tttacatttt gcattgcaat
      481 tctaaataaa gtcattttta ttaactcctc caattaagga aactgtattt actattttat
      541 tatagaactc tctgttactt tctcccttta caatttctgt gaatattatt ccatccttta
      601 taaagagtat tctattgcaa aaactcgcac ttatagcatc atgagttacc atcaatattg
      661 ttttattgct gtctttgttt aattttaata gacaattcaa caaatcccta gcagatttag
      721 aatctaatgc tcctgtaggc tcatctgcaa gtactaatga tggttttgtt gctatagctc
      781 tacatacagc tcctctttgc ttttgtccac cagaaacttc gtcagggtat ttatctaata
      841 tagattctat atttaattct tttgaaatct cttttattct attttctatt actgatactc
      901 tttcattttt taaagaaaga ggcaacatta tattttcttt tattgtaagt gtatctaaaa
      961 gattagaatc ttgaaataca aagcctagat tatcacgtct aaaatctgcc aattcatttt
     1021 gcttcatttt aatagtattc tttccattta tataaatatc tcctgatgaa ggtaaatcta
     1081 ttgtagaaat aatatttaaa agtgttgatt taccagcacc ggaagacccc ataataccaa
     1141 caaattctcc tttataaacc ttgaaagtta ttcctcttag agcttcatgt tttgaacctt
     1201 ttcttccata ctcctttttt atattttcaa cagataatat ttcttccata gttaatattc
     1261 acctttcatc acaattagtt atttatcaat tctcacaaat atattatata tattaaaatg
     1321 gcttttaaac atttaattat attaatttaa ggttacattt tcgtaagtat taattatatt
     1381 tcttcaaatt tattgcaatt atgtttaaaa tataatttat caagtaaaat atagttatat
     1441 taatataatt attttaggta ataaggtaga aatattttaa tttatcaagt agaatatagt
     1501 tatgttaata taactatttt gaattaggag gtaagaatgt ttaaaataat ggtagttgaa
     1561 gatgacgtct cattgaaaaa tatcatagca aagtgtctta ctaaatgggg acatgatgtt
     1621 catcaaatag aaaatcttga aaatataata gaagagttca aaaactataa cccagaatta
     1681 gttttattgg acataaacct tccattttat gatggttttc attggtgtaa tgaaataaga
     1741 aaaatttcaa aagttcctat aatttttatt tcttcaagaa attctaatat ggatgtaata
     1801 atgggtgtaa atttaggtgc agatgattat atacaaaaac ctttttctgt agatgtctta
     1861 gtagctaaag ttaatgccct tttaagaaga acttataatt ttgttgataa taactcaaat
     1921 cagattattc acaatggtgt tactttagat ttatcaactg caacaataaa ctatgaagat
     1981 aatactattg agctaacaaa aaatgaaatc aaaattctcc atgagcttat gaaatataag
     2041 gggcagatag taagtaggaa taaactcatg aaaaaattat gggataatga ttggtttgta
     2101 gatgataata cattaactgt caatgtaaat agaatacgct caaaattaaa tgaaattggt
     2161 ttagaagatt ttatagaaac aaaaagagga ttgggttata taatcagtta aaacaattct
     2221 aagtaggtga atttatgagc tttaaagatt ttctcaagga taaactaggt tttttagtat
     2281 acaattttac tttcttaatt tttacagtat cagttattat attttctcct gtagatgttt
     2341 ttctgactga tactatttta tatatattag ttgtaaatgt tgtgttttta tttttatatc
     2401 tatttattag ctacatcaag aagaacaaat ttttagataa gataaaaaat gatacattca
     2461 aaattaatat gaatgatata gaattagcta gatgtaaaaa tgaagaaaaa atatacttga
     2521 acattattaa aaatcatcaa tatcaatgta ataacataat aaataataat atcgagaaat
     2581 ttgaagaaaa caaagagatt ttaaatatgt gggtacacga tgtaaagata cctatagcta
     2641 taataaaact cattcttgaa caaaatgaaa atttaattga tgaaaaaatt tcagatgaca
     2701 tagatagtga aatatttaga attgaaaact ccgttgaaaa aattttatat ttatcaagac
     2761 tcgaagattt tcataaggat tttttaattc aagaggtaaa tattgaaaaa atcattagaa
     2821 atattataaa aaaatactca aaatatttta tttcaaaaaa aattatttta gacttaaata
     2881 atttgaattt taaggtttta tctgatgaaa aatggctata cttcatattc gagcaattat
     2941 tgagtaattc cctaaaatac tcctcattag gagataatat atctatctat tgcaaaagaa
     3001 ccaaaaatta tttacaatta agagtggaag atactggttg tggtataaaa aaggaagatt
     3061 taaatcgaat attcaataag ggatttacag gtaataatgg aagaaacaat tctaaatcta
     3121 caggtcttgg gctttattta gtaaaagaac tttgtgataa gcttggtcat aaaattgatg
     3181 tagattcaga gtacaaccaa tatacaaaat ttactattac attcaaatgt gatttatagt
     3241 atcttattat tgatagttta ttttcattaa cctatattca acttcttata gtaattctat
     3301 atgtacatcc cagtatataa ttaagtagtt tttataaaaa ggaggaagac atgtcttttc
     3361 taaaaaaatt taaaaataaa agatttagaa ataacagttt taaaagtaac aactttagaa
     3421 atagcagttt tggaagtagt ggcttcaagt ctaaaaataa aggttttaaa aacaatccct
     3481 ttaaaaataa gagattcaaa atatatgcag gaatagcagt aatcatactt ttaattctag
     3541 gagttcttgc ttatgttgat tcgaaaaaca ctgaattaca agctaatgaa aattttatcg
     3601 aaacatatac tattcctgaa aatgaaaaaa tatttataaa tgggatggtt gttcctaagc
     3661 aaactaagga cttcaatata tctggagatt atgagttaag tgatgttaat gttacaaatg
     3721 gtcaaaaagt aaatcaagga gaccttcttt ttactgctaa aaatccaact ataatagcag
     3781 aaatagatag tttaaaatca caacttagtc aatacaaaaa acaaaaaata tcactttctg
     3841 atattgctga aaataaagat gctattgctt ctataaatgc acagataact gctttaaact
     3901 ctcagatagc ttctctggat aaaaaagctt atgacagagt tactgctcct tttgatggca
     3961 cagtatactt gaatgaccaa actggaaatc cagaccaacc agtttcattt atgactattc
     4021 aaggtctaga gttttatatg aaaggacaag caagtgaaca agatttacct aaaatgaaga
     4081 ttgaccaaat ggtcaatata ctagtttttt caactgacca aaagcttact ggtagaataa
     4141 gttttatatc tgataaacca tctactccaa atactgaaat gggagctcag cagaatacac
     4201 tatcttatta tgatataaac atagcatttg ataatcaaga aggattagta aatggattcc
     4261 atcttcaagc atcacttgaa gtagctaatt caagctttaa aataccagca tcttgtgtac
     4321 ttaaagacaa caaacactca tatgtattta aggatttaga tggtatattg aaaaagcaaa
     4381 ttgtagatgt agcaagtcaa aatgatgatt ttgctgtagt tagaggcggt cttgaacaag
     4441 gcgatataat aataaaacac cctactaaag aaatgaaaga aggagaccca gttcaaggcg
     4501 atggcgtaac tgctggttct aataatggag atactactcc aaacaaaaaa gtagaagaag
     4561 ttactatgga tgtgaattaa tttacatatg ttaattaaat tagaaaatat tcaaaaatat
     4621 tacaaagtag ggaaagatga attacatgtt ttaaaatcct taaatttaga aattgaatct
     4681 ggagaatttg tcatgataat gggtaagtct ggtagtggta aaactacttt acttaatatc
     4741 ttaggttttc tagatgtatt tgatgaagga agatatatat ttgatggaac agatgtaact
     4801 aatttgagtg aaaatgaacg ttctgttttt agaaatatta atattggatt tgttttccaa
     4861 caatttaatt taatagaaac tctaaatgta tatcaaaatg tagagttgcc tctaatttac
     4921 aataaagcac taaaaaaatc taatagagaa gaaattgtta aagataaatt aagttctgtt
     4981 gggcttttgg acaagcttaa gcagaaacca cttcaattat ctggtggtca acaacagcgt
     5041 gtagccattg cacgttgcct agcaaatgac cctcagataa tctttgctga tgaaccgact
     5101 ggggcattag atagtgaaac tagcagagaa attatggagc ttttaacaag gctcaacaaa
     5161 caaggtaaga ctataataat ggttacacat gatcaagatt taactaagta tgcaactaag
     5221 gttatacgac ttaaagatgg tgtgtttact tcggaggtgt aatcatgagt ttattaaaaa
     5281 attctttggc caatttaaaa ggtcacaagc ttcgtgtatt tgtagcttta ctctggatta
     5341 taataggaat aacttcagta atacttgtta gttctatagg taatggattt caaaaagaaa
     5401 ttaagaaatc tgtaaacaat gtaaacccta ataaaactac tatttcgttt gaatcagccg
     5461 ataacacagg tcttactgat gatatgagta tttttttaaa accatttaat gcaaaagatt
     5521 tagaagaatt atcatttgtt gaaggagtag aaagaatagc tccttcaaga gatgggttta
     5581 accttgattc agtttactct tcacaagcat cttttgataa aaaaactacg tatgttgatg
     5641 ttggaccagt aaaaaaagat tctaaaataa atttaatttg tggtagagat ttttctttag
     5701 atgatgaaaa aagaaaagta attttactta cattacaaag tacaagtgaa atttttgaaa
     5761 atccagaaga tgccttaggt catggtatca acataaatgg aactatattt gaaataatag
     5821 gggtattaga tgattctcag caaaaccaag ctggaggatt ctttggaggt taccaagata
     5881 tgcaatttac tacttcccta gtacctaaaa aggcttttga cactttaatg agtcaaaatt
     5941 cttattcaaa tgaaatttac cagttagact tagtttcttc taaaggctat aatgtaaatg
     6001 aagttgcaaa caatgttata gctaaattat atgagatgca ccctggtatc aatggttctt
     6061 atactacacc agacccaact gaacagacag cttatcttga aagcattaat tccaatgtaa
     6121 acaaatatgt atctataata actgtagttg caatgtttgt tggtggtatt ggtgtaatga
     6181 atataatgta tgtatctgtt atggaaagac aaagagaaat tggtataaga cgtgctattg
     6241 gagcaaaacc taggtctata ttatttcaat ttttagttga agctgttttt ataacagtat
     6301 gtggtggaat tttaggaact atagttggat ttgctgctac taactatgtt tctaaatata
     6361 taggttttga agctatacct agcttaaata gcttatttta tgcaattgta gctactatat
     6421 tgactggagt tgtatttggt ctgattccag cctttaaagc ctctaaacta gaccctataa
     6481 aggcaattta taagtagaaa ttgtataaag attttcgatt tttagattag tttatataaa
     6541 acatacttaa atatataacg cactattata tatgatagat taggaactta atctttctaa
     6601 atataatagt gcgttctttc ataaaaagga ttctcttcct tactatatct aaattaccaa
     6661 gatgttatgt tctaattgtt caaactaata cttccaattt tgattaatca atctcattct
     6721 cctaatatac aatcttttac tttcaatcat tgtacactcc gccttaaaaa aataatctta
     6781 aattaggcgt tatatacaat ctccccatta catattgtat aattaacctt tccatataaa
     6841 gatttacctt taaacggtga attatcagct ttagaaacaa agctttcaac tgtccattct
     6901 tcttctggat tgaatataac aatgtctgct acagcccctt cttctataaa tccaatattt
     6961 aaattgtaca actttgcagg atttatagac attttttcta ttaactgcat catactaaga
     7021 tgatttttat gcaccaaatt tgttattcca agagctaaag aagtttcaag cccaataatt
     7081 ccacttggag cttttataaa ctctctatct ttttcttctt tactatgagg tgcatggtcc
     7141 gttgctatta tttcaatagt accatcattt aatccctcta taatcttatc tctgtcccat
     7201 ttacttctta gaggtggatt catttttgca tttgttccaa actccaatac atcttcctca
     7261 gtcaatgtaa agtgatgtgg tgaagcttct gcaactacat ttgcacctaa gtattttgca
     7321 aatctaacca tatcaacaga tacacctgaa cttatatgtt gtatatcaac tttagcacca
     7381 gtctttagtg ccaacataca atctctggct accataacat cttctgctac acttgatgca
     7441 ccttttaatc ctagttctga ggctaccttt cctgcattta cacctggatt tccaactaaa
     7501 gaagggtctt cttcatgaaa actaagtggt acatccaact cttttgcctt aatcattgcc
     7561 tccataatta gattgctatc cattaaaggt aatccatcat cagtaaaccc tggtacacca
     7621 gcttttttaa gagcttccat atcaactaac tctttacctt caaaaccttt agttatagct
     7681 gctgcttgta aaacatttat acatgcattt tttgactttt ctttaacata attaaaagta
     7741 tcttcattat ctacaatagg attagtattt gccatacata taacagttgt aaatccacct
     7801 ctagcagcag atttagctcc actttctata tcttctttat atgtaaaccc agggtctcta
     7861 aagtgtacat gtacatccac taaacctgga gcaactacat taccactagc atctattatt
     7921 ttttcatatt catcactttc atcaaacttg cctattttat aaattttatt ttcttttata
     7981 attaagtcaa tattctcatc tctttgactt ttagggtcta ttactctacc gtttttaatt
     8041 aaaatcatat tatttctcct ttatattttt aatttatata gactcatata atattataca
     8101 ttattttaca tacttaaata caaaattacg tttgacatca atagcctttt taggacaaag
     8161 ttcatggcaa cagtaacatc ttatacattt acttaagtct ataatagcac ccttagtccc
     8221 tatctctata gtggatgcag gacaagcttc cttgcatttt ccacatttta tgcaatcttg
     8281 aaatcttacc acaggctttg gtgttataag ctttgaaata ggttcattta aaaattttgg
     8341 tagctttacg gaaaacaatc ttaaatcctt acttacattt ggaatcttgt aattttttat
     8401 aattaaatct tttatatctt cacctaaaat ttcaatatct gaaaaatctt cttgtatata
     8461 tcctctctta atagcacctc taattgtagc aactctattt ggaagtaaac ttataatctt
     8521 acatgcaaca acatctatag catatggatt ttgtgatgct aataaaactc ctatttctcg
     8581 agtagtacca gctgaaggtc catcaccttc catacctact ataccatcca taattgtcaa
     8641 tgttggtttt atataatcac aaatatctaa aagtacattt tcgcaaaaca actcttctgt
     8701 aggaaatcta taatgtatct ctgctttttt caatccagct atgcatccat ataagttttt
     8761 tgtaccacca gtaaaagttg ccattatatg agtctttaat ttacaaagat ttattatata
     8821 atctgaatct actattggtt taataatatc catatatttt agaacatgtg ctttttcact
     8881 atgtatctta acatctgaaa catcataatt taacttaatg cccagttcat ttgctacttc
     8941 ttcaattcca gatgccttat aaatgctctt aagagaactt tgattatatg gaccaccagg
     9001 gctatctgct attgtaatat cacaatttaa ctctaaaagt ttttcacata caacttttac
     9061 aaccatagga tgtgtagttg tagcttcttc aggctttttt ttcattaata aatttggttt
     9121 tataagtact ttgcttcctt ttttaacaaa cttatctata ccaccaatat cctctaaatt
     9181 tttttcaata gatagcttta cttcttcata attgtaagtt ttacaactcc taacagatac
     9241 cgacttcata ttatctccat ccttctatca caacagttta tttgtataat ttaaaaaagg
     9301 agaaactaaa gtttctcctt ttaatctatg ttttaattgt tttaattatt attttccaaa
     9361 atgtctgttt aataatccta agaatgcttt accatgtcta gcttcgtctt tacacatttc
     9421 atgtactgta tcatgtatag catctaatcc taattcttta gctctcttag ctaattttaa
     9481 ttttccatca gttgcaccat actcagcatc aactctaact cttaagtttt cttttgtatc
     9541 tgcaactaca acttctccaa gaagttctgc aaatttagca gcatgttcag cctcttcaaa
     9601 agctattctc ttatacgctt cagctacttc tggataacct tctctatcag cttgtctact
     9661 cattgctaaa tacattccta cttctgtaca ctcaccagta aagttagctc ttaatccttc
     9721 gattatttct gcatctacac cttgagctac tcctattcta tgttcatcag cccaaaccat
     9781 ttcgcctttc atttcttcaa atttatcagc tccaacttta catactggac attgtgctgg
     9841 tgcagcatct ccttcatgta tatatccaca tactgtacaa acaaattttt tcataattaa
     9901 tttcctccca attttatata attaaatttt tatttttaag tttaataact tgctacaaga
     9961 atatatatac cccagacttt atagcttaaa cactaaaaat aaaatttatt tcctaattaa
    10021 ttttatcact ttgaaacacc agtatcaacc aagttttgat gtcaaatttc tacataatac
    10081 ataatttatt tgattaatta ggaaatagtt gtcatattaa atactttcta atgaagactc
    10141 ctctgaatct ttaagtttca cttttttgct ctttaaaaaa gataatttag tttctgcagt
    10201 tatgattgca acaaatatca agatactacc aataaccaat tttgctgtaa taagctcctt
    10261 taaaattatt atagaaaata ttgtaccaaa tacagcctct gtggataata ttatagctgt
    10321 tctagttcca tcaactcttt tttgacatat agtctgaaat aaaaatccaa ttgtagtact
    10381 aaatacagct agatacatag ttcccatgta accagataat tcagcttcca tctttaattg
    10441 acctgcaaat gtttgaacta caacagacat taaaaacgct actgtaaatt gaatagctgt
    10501 caatgccatt gggttgttat cttttgcaaa ctcacttgta aagaatatat gaaatgcaaa
    10561 tccgaatgaa catataagtg ttagaaaatc tccaaaatta atagagaaat cagcttctaa
    10621 agaaagtatc ccaattccta taagagctac caaactactt attattccta ttttatcaag
    10681 ttttcttcta taaagtataa atcctatgaa cggaactatt acaacattag ctgctgttat
    10741 aaaagcattt ttagatggtg tagtgtacat aagtccaata gtttgtactg tgaatgctag
    10801 aaataaaaat attcctaaaa tccctcctgc ttttaataat tttttaccca tattggcacg
    10861 aatctgttta aaaaacagta aattcataat tatagctgcc aagaaaaatc ttaaagttat
    10921 gatttgtaga ggtgtaagcc caccgtctaa tgcaaattgg gttgctacaa agccacttcc
    10981 ccatataatt gctataaata ttagtcctat ttctccaaag tattttttca ataaagcccc
    11041 ctccttttta tttttttgca aaattataaa ccccctaaac agcaagagag tttttctata
    11101 agcattacta taaattaata tagtaaattt tttcagtatt ttaaatctaa atttacatac
    11161 attttgagct caaatttata tccactattt aacagtaata tattaatcat tttttgtaaa
    11221 caccttttta ataaattttt atatttttta tattttttta cctatactaa atttttttat
    11281 agatattttt aatatatatg atactttaat attatgcaaa taatgtcata taagttacac
    11341 tttagttatc ttagttacaa ctttgtcggt ttactttttc tactttcttc gatataatta
    11401 taatatattt ttgatacaat tcgacaaaag ggggaatgct gtaattatgg taaaaaaaag
    11461 aaaaaagaaa actgttacac ttactatact tgttgtaata gttattggta ttttttctat
    11521 ggcatgggcg gacatgtcta gaaaaaaaga aatgaaagaa actcaagaaa gacttaaagc
    11581 tgaaagatta gaaaaagaaa gaattgaaaa agaaaaaaat gaacctatta ttggtgcaaa
    11641 aaaagaagct gtaaaagaat atggatatga cgcaaaaata gtttgggaca aacttaacaa
    11701 atacgactat tcaaacaatg gagaaaagat agtctttctt acatttgatg atggaccatc
    11761 tactactaat actccacaag ttctagatat cttgaaaaga catggtgtta gaggaacttt
    11821 ctttattaag ggtgattctc ttgaaagaaa aggagctaat gaaattttaa aaagaacatt
    11881 tgacgaagga aatgcaatag cccatcactc ttatactcat gactataaaa aactatatcc
    11941 tggcagaagc ctaaatctcg atacattcgt aaatgaactt aacaaaactg atgaagctat
    12001 gaaaaaagtc ttaggaaaga atttttcctc aaatgtagtt agatgcccag gcgggtatat
    12061 gtcatggaaa aatatggaac ctctaggaaa ctacttaaaa gagaaaaaca tggcatcaat
    12121 tgattggaac gctttaaatg ctgatgcaga aggtaaaaag aaaaatgcac aagaattatt
    12181 tgaacatgca aagaaatctt ctgaaggcaa agaaatggtt gttcttttaa tgcatgatac
    12241 ttatggaaaa caagaaacag taaatgcatt ggaccaaatt ataacatatt ttaaagacaa
    12301 tggttatcaa tttaaaattt taatataata aaaaagcaag tttattgaat atacatttta
    12361 tactcaatag acttgctttt actttactct attttactag ctcgattttt acaaattttt
    12421 tctttcctat ttgaacaact aattcactct cttttacaat agtgcttaaa tcctctactt
    12481 tttcaccatt tacctttact ccaccttgag aagctaatct tctaacttca ctcttactct
    12541 taacaatctc attagaaact agtacttcga ttaagtcaaa tccatcttcc ttaacttgaa
    12601 ttgtttgtat atcttcaggt atttgacctt tttggaatac agatttaaat ctttcttcag
    12661 cttctttagc agattcttct ccatggtata atgttactat ttctcttgct aagttcatct
    12721 ttatatctct tggatttact gaaccctctt tcaactgaga ttctattcta ttaacttcat
    12781 caggatgaac atctgtcact aaattgtagt atttaattat aagttcatca ggaatttcca
    12841 tagatttttg gtacattatt cccgcttctt catctattcc aatatagtta cctaaactct
    12901 tactcatttt ttctttacca tctaatcctt ctattaaagg catcattatt actatttgag
    12961 attccatacc aaattctctt tgtaatgatc ttcccattag taaattgaat ctttggtcag
    13021 ttccaccaag ttctatatca gcttctaatg ctatagagtc atatgcttgc attaatgggt
    13081 agaagaactc atgtactgat attggcattt gtccttcata tctcttttta aaatcttctc
    13141 tttctaacat tctcgcaaca gtaatagttg ctgctaattt tattacgtct tcaaaattta
    13201 acttggctaa ccactcgcta ttaaatctta caatagtttt ttctttatct aaaactttga
    13261 aaatttgttc ttcataagtt tttgcatttg caagaacttg ctctgttgtt aaagcttttc
    13321 tagctttaga ttttccagtt gggtctccaa tttttcctgt aaaatctcca attattatta
    13381 ctatttgatg acctaaatct tgtaattgtt tcatctttct aagaactact gtatgcccta
    13441 aatgtatatc tggagcactt gggtctagac ccaattttac tatcataggc tttccttctt
    13501 tttctgattt aataagcttt tcccttaact ctttctcatc tattaaatca tctacacctt
    13561 tcataattat tcgcatttgc tcgtctatcg atttcatttt tcattcctcc taaaatattt
    13621 atataatcta taatttttat ttttataata aaaaaagcct tcatcctctt aaaaggacga
    13681 aagcattgct tacgtgttac caccttagtt catcaatatc tcacgatatt aatctcttag
    13741 agtacaatta catatactcg tgtactataa cgtgtactaa tccgatatct cttaatggtt
    13801 gttatctttc agagatagtg ctctgagatg tattcaaact aaatcaattt attccatttc
    13861 accaaactgg aactctctgt taaataaaat ctagccctac ttcttctctt catagcattt
    13921 atatattcag attatttcat caattacaag aaatttttct taacttactc ataatatatt
    13981 attattttta tattttgtca acctttttta ttattatata catgtttttt tacattatac
    14041 tactttttta tattttttac caatacccaa aatttatttt tcaatcaaaa gatttatttg
    14101 tttattggct atattactct gactcaatct tatatttatt ttcttattta aaaatgcgtt
    14161 tataattatt aaaccaataa ctgttacatc aaat
//
LOCUS       DAEMHW010000099        14061 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEMHW010000099 DAEMHW010000000
VERSION     DAEMHW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14061
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.099"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            187..741
                     /gene="tcdR"
                     /locus_tag="KPK67_002374"
     CDS             187..741
                     /gene="tcdR"
                     /locus_tag="KPK67_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBG2459331.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            1059..8159
                     /gene="tcdB"
                     /locus_tag="KPK67_002375"
                     /gene_synonym="toxB"
     CDS             1059..8159
                     /gene="tcdB"
                     /locus_tag="KPK67_002375"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBG2459332.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIQEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIESDLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSYFISFEDISETEEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKVMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYIFVDSEGKENGFINGSTKEGLFVSELLDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGSTNTSDSLMNFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFEDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDDKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8281..8781
                     /gene="tcdE"
                     /locus_tag="KPK67_002376"
     CDS             8281..8781
                     /gene="tcdE"
                     /locus_tag="KPK67_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBG2459333.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKGEK"
     gene            8860..9021
                     /locus_tag="KPK67_002377"
     CDS             8860..9021
                     /locus_tag="KPK67_002377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459334.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYMDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            9110..9292
                     /locus_tag="KPK67_002378"
     CDS             9110..9292
                     /locus_tag="KPK67_002378"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459335.1"
                     /translation="MRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9509..>14061
                     /gene="tcdA"
                     /locus_tag="KPK67_002379"
                     /gene_synonym="toxA"
     CDS             9509..>14061
                     /gene="tcdA"
                     /locus_tag="KPK67_002379"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBG2459336.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     LLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQIKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTSNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFM"
BASE COUNT         5712 a         1445 c         2214 g         4690 t
ORIGIN      
        1 ctaaaatact ttatttatta gaaaaagatt actaattaat tattaaaatt aatgtattca
       61 taatgcatat tttcatataa aatttaattt atttgccgat tatataatta taatgactga
      121 tttaattcca atgttgtcaa aattttcaaa taaatcatca ttataaatat aagagaggat
      181 gattttatgc aaaagtcttt ttatgaatta attgttttag caagaaataa ctcagtagat
      241 gatttgcaag aaattttatt tatgtttaag ccattagtaa aaaaacttag tagagtttta
      301 cattatgaag agggagaaac agatttaata atatttttta ttgaattaat aaaaaatatt
      361 aaattaagta gcttttcaga aaaaagcgat gctattatag tcaaatatat tcataaatca
      421 ttactgaata agacttttga gttgtctaga agatattcta aaatgaagtt taattttgta
      481 gaatttgatg aaaatatctt aaatatgaaa aataattatc aaagtaagtc tgtttttgag
      541 gaagatattt gttttttcga atatattttg aaagaattat ctggtattca aagaaaagtt
      601 attttttata aatatttaaa aggatattct gatagagaaa tatcagtgaa attaaaaata
      661 tctagacaag ctgttaataa ggctaaaaat agagcattta aaaaaataaa aaaagactat
      721 gaaaattatt ttaacttgta attaatgagc ttaaagaaat atttacaata gaaatcaaat
      781 tttagaatta actttattgt aaaatcaata acttaatcta agaatatctt aatttttata
      841 ttttatatag aacaaagttt acatatttat ttcagacaac gtctttattc aatcgaagag
      901 caaattaatc aactgagtgt cttcaattta aaatgttagg aagtgaatgt atatgaaaac
      961 ctaagtagat attagtatat tttataaata gaaaggagga tatataaaag agttttagca
     1021 tttagatgta aaaatattat agtaaaggag aaaattttat gagtttagtt aatagaaaac
     1081 agttagaaaa aatggcaaat gtaagatttc gtactcaaga agatgaatat gttgcaatat
     1141 tggatgcttt agaagaatat cataatatgt cagagaatac tgtagtcgaa aaatatttaa
     1201 aattaaaaga tataaatagt ttaacagata tttatataga tacatataaa aaatctggta
     1261 gaaataaagc cttaaaaaaa tttaaggaat atctagttac agaagtatta gagctaaaga
     1321 ataataattt aactccagtt gagaaaaatt tacattttgt ttggattgga ggtcaaataa
     1381 atgacactgc tattaattat ataaatcaat ggaaagatgt aaatagtgac tataatgtta
     1441 atgtttttta tgatagtaat gcatttttga taaacacatt gaaaaaaact gtagtagaat
     1501 cagcaataaa tgatacactt gaatcattta gagaaaactt aaatgaccct agatttgact
     1561 ataataaatt cttcagaaaa cgtatggaaa taatttatga taaacagaaa aatttcataa
     1621 actactataa agctcaaaga gaagaaaatc ctgaacttat aattgatgat attgtaaaga
     1681 catatctttc aaatgagtat tcaaaggaga tagatgaact taatacctat attgaagaat
     1741 ccttaaataa aattacacag aatagtggaa atgatgttag aaactttgaa gaatttaaaa
     1801 atggagagtc attcaactta tatgaacaag agttggtaga aaggtggaat ttagctgctg
     1861 cttctgacat attaagaata tctgcattaa aagaaattgg tggtatgtat ttagatgttg
     1921 atatgttacc aggaatacaa ccagacttat ttgagtctat agagaaacct agttcagtaa
     1981 cagtggattt ttgggaaatg acaaagttag aagctataat gaaatacaaa gaatatatac
     2041 aagaatatac ctcagaacat tttgacatgt tagacgaaga agttcaaagt agttttgaat
     2101 ctgttctagc ttctaagtca gataaatcag aaatattctc atcacttggt gatatggagg
     2161 catcaccact agaagttaaa attgcattta atagtaaggg tattataaat caagggctaa
     2221 tttctgtgaa agactcatat tgtagcaatt taatagtaaa acaaatcgag aatagatata
     2281 aaatattgaa taatagttta aatccagcta ttagcgagga taatgatttt aatactacaa
     2341 cgaatacctt tattgatagt ataatggctg aagctaatgc agataatggt agatttatga
     2401 tggaactagg aaagtattta agagttggtt tcttcccaga tgttaaaact actattaact
     2461 taagtggccc tgaagcatat gcggcagctt atcaagattt attaatgttt aaagaaggca
     2521 gtatgaatat ccatttgata gaatctgatt taagaaactt tgaaatctct aaaactaata
     2581 tttctcaatc aactgaacaa gaaatggcta gcttatggtc atttgacgat gcaagagcta
     2641 aagctcaatt tgaagaatat aaaaggaatt attttgaagg ttctcttggt gaagatgata
     2701 atcttgattt ttctcaaaat atagtagttg acaaggagta tcttttagaa aaaatatctt
     2761 cattagcaag aagttcagag agaggatata tacactatat tgttcagtta caaggagata
     2821 aaattagtta tgaagcagca tgtaacttat ttgcaaagac tccttatgat agtgtactgt
     2881 ttcagaaaaa tatagaagat tcagaaattg catattatta taatcctgga gatggtgaaa
     2941 tacaagaaat agacaagtat aaaattccaa gtataatttc tgatagacct aagattaaat
     3001 taacatttat tggtcatggt aaagatgaat ttaatactga tatatttgca ggttttgatg
     3061 tagattcatt atccacagaa atagaagcag caatagattt agctaaagag gatatttctc
     3121 ctaagtcaat agaaataaat ttattaggat gtaatatgtt tagctactct atcaacgtag
     3181 aggagactta tcctggaaaa ttattactta aagttaaaga taaaatatca gaattaatgc
     3241 catctataag tcaagactct attatagtaa gtgcaaatca atatgaagtt agaataaata
     3301 gtgaaggaag aagagaatta ttggatcatt ctggtgaatg gataaataaa gaagaaagta
     3361 ttataaagga tatttcatca aaagaatata tatcatttaa tcctaaagaa aataaaatta
     3421 cagtaaaatc taaaaattta cctgaattat ctacattatt acaagaaatt agaaataatt
     3481 ctaattcaag tgatattgaa ctagaagaaa aagtaatgtt aacagaatgt gagataaatg
     3541 ttatttcaaa tatagatacg caaattgttg aggaaaggat tgaagaagct aagaatttaa
     3601 cttctgactc tattaattat ataaaagatg aatttaaact aatagaatct atttctgatg
     3661 cactatgtga cttaaaacaa cagaatgaat tagaagattc ttattttata tcttttgagg
     3721 acatatcaga gactgaagag ggatttagta taagatttat taataaagaa actggagaat
     3781 ctatatttgt agaaactgaa aaaacaatat tctctgaata tgctaatcat ataactgaag
     3841 agatttctaa gataaaaggt actatatttg atactgtaaa tggtaagtta gtaaaaaaag
     3901 taaatttaga tactacacac gaagtaaata ctttaaatgc tgcatttttt atacaatcat
     3961 taatagaata taatagttct aaagaatctc ttagtaattt aagtgtagca atgaaagttc
     4021 aagtttacgc tcaattattt agtactggtt taaatactat tacagatgca gccaaagttg
     4081 ttgaattagt atcaactgca ttagatgaaa ctatagactt acttcctaca ttatctgaag
     4141 gattacctat aattgcaact attatagatg gtgtaagttt aggtgcagca atcaaagagc
     4201 taagtgaaac gagtgaccca ttattaagac aagaaataga agctaagata ggtataatgg
     4261 cagtaaattt aacaacagct acaactgcaa tcattacttc atctttgggg atagctagtg
     4321 gatttagtat acttttagtt cctttagcag gaatttcagc aggtatacca agcttagtaa
     4381 acaatgaact tgtacttcga gataaggcaa caaaggttgt agattatttt aaacatgttt
     4441 cattagttga aactgaagga gtatttactt tattagatga taaagtaatg atgccacaag
     4501 atgatttagt gatatcagaa atagatttta ataataattc aatagtttta ggtaaatgtg
     4561 aaatctggag aatggaaggt ggttcaggtc atactgtaac tgatgatata gatcacttct
     4621 tttcagcacc atcaataaca tatagagagc cacacttatc tatatatgac gtattggaag
     4681 tacaaaaaga agaacttgat ttgtcaaaag atttaatggt attacctaat gctccaaata
     4741 gagtatttgc ttgggaaaca ggatggacac caggtttaag aagcttagaa aatgatggca
     4801 caaaactgtt agaccgtata agagataact atgaaggtga gttttattgg agatattttg
     4861 cttttatagc tgatgcttta ataacaacat taaaaccaag atatgaagat actaatataa
     4921 gaataaattt agatagtaat actagaagtt ttatagttcc aataataact acagaatata
     4981 taagagaaaa attatcatat tctttctatg gttcaggagg aacttatgca ttgtctcttt
     5041 ctcaatataa tatgggtata aatatagaat taagtgaaag tgatgtttgg attatagatg
     5101 ttgataatgt tgtgagagat gtaactatag aatctgataa aattaaaaaa ggtgatttaa
     5161 tagaaggtat tttatctaca ctaagtattg aagagaataa aattatctta aatagccatg
     5221 agattaattt ttctggtgag gtaaatggaa gtaatggatt tgtttcttta acattttcaa
     5281 ttttagaagg aataaatgca attatagaag ttgatttatt atctaaatca tataaattac
     5341 ttatttctgg cgaattaaaa atattgatgt taaattcaaa tcatattcaa cagaaaatag
     5401 attatatagg attcaatagc gaattacaga aaaatatacc atatatcttt gtagatagtg
     5461 aaggaaaaga gaatggtttt attaatggtt caacaaaaga aggtttattt gtatctgaat
     5521 tacttgatgt agttcttata agtaaggttt atatggatga tagtaagcct tcatttggat
     5581 attatagtaa taatttgaaa gatgttaaag ttataactaa agataatgtt aatatattaa
     5641 caggttatta tcttaaggat gatataaaaa tctctctttc tttgactcta caagatgaaa
     5701 aaactataaa gttaaatagt gtgcatttag atgaaagtgg agtagctgag attttgaagt
     5761 tcatgaatag aaaaggtagt acaaatactt cagattcttt aatgaacttt ttagaaagta
     5821 tgaatataaa aagtattttc gttaatttct tacaatctaa tattaagttt atattagatg
     5881 ctaattttat aataagtggt actacttcta ttggccaatt tgagtttatt tgtgatgaaa
     5941 atgataatat acaaccatat ttcattaagt ttaatacact agaaactaat tatactttat
     6001 atgtaggaaa tagacaaaat atgatagtgg aaccaaatta tgatttagat gattctggag
     6061 atatatcttc aactgttatc aatttctctc aaaagtatct ttatggaata gacagttgtg
     6121 ttaataaagt tgtaatttca ccaaatattt atacagatga aataaatata acgcctgtat
     6181 atgaaacaaa taatacttat ccagaagtta ttgtattaga tgcaaattat ataaacgaaa
     6241 aaataaatgt taatatcaat gatctatcta tacgatatgt atggagtaat gatggtaatg
     6301 attttattct tatgtcaact agtgaagaaa ataaggtgtc acaagttaaa ataagattcg
     6361 ttaatgtttt taaagataag actttggcaa ataagctatc ttttaacttt agtgataaac
     6421 aagatgtacc tgtaagtgaa ataatcttat catttacacc ttcatattat gaggatggat
     6481 tgattggcta tgatttgggt ctagtttctt tatataatga gaaattttat attaataact
     6541 ttggaatgat ggtatctgga ttaatatata ttaatgattc attatattat tttaaaccac
     6601 cagtaaataa tttgataact ggatttgtga ctgtaggcga tgataaatac tactttaatc
     6661 caattaatgg tggagctgct tcaattggag agacaataat tgatgacaaa aattattatt
     6721 tcaaccaaag tggagtgtta caaacaggtg tatttagtac agaagatgga tttaaatatt
     6781 ttgccccagc taatacactt gatgaaaacc tagaaggaga agcaattgat tttactggaa
     6841 aattaattat tgacgaaaat atttattatt ttgaagataa ttatagagga gctgtagaat
     6901 ggaaagaatt agatggtgaa atgcactatt ttagcccaga aacaggtaaa gcttttaaag
     6961 gtctaaatca aataggtgat gataaatact atttcaattc tgatggagtt atgcaaaaag
     7021 gatttgttag tataaatgat aataaacact attttgatga ttctggtgtt atgaaagtag
     7081 gttacactga aatagatggc aagcatttct actttgctga aaacggagaa atgcaaatag
     7141 gagtatttaa tacagaagat ggatttaaat attttgctca tcataatgaa gatttaggaa
     7201 atgaagaagg tgaagaaatc tcatattctg gtatattaaa tttcaataat aaaatttact
     7261 attttgatga ttcatttaca gctgtagttg gatggaaaga tttagaggat ggttcaaagt
     7321 attattttga tgaagataca gcagaagcat atataggttt gtcattaata aatgatggtc
     7381 aatattattt taatgatgat ggaattatgc aagttggatt tgtcactata aatgataaag
     7441 tcttctactt ctctgactct ggaattatag aatctggagt acaaaacata gatgacaatt
     7501 atttctatat agatgataat ggtatagttc aaattggtgt atttgatact tcagatggat
     7561 ataaatattt tgcacctgct aatactgtaa atgataatat ttacggacaa gcagttgaat
     7621 atagtggttt agttagagtt ggtgaagatg tatattattt tggagaaaca tatacaattg
     7681 agactggatg gatatatgat atggaaaatg aaagtgataa atattatttc aatccagaaa
     7741 ctaaaaaagc atgcaaaggt attaatttaa ttgatgatat aaaatattat tttgatgaga
     7801 agggcataat gagaacgggt cttatatcat ttgaaaataa taattattac tttaatgaga
     7861 atggtgaaat gcaatttggt tatataaata tagaagataa gatgttctat tttggtgaag
     7921 atggtgtcat gcagattgga gtatttaata caccagatgg atttaaatac tttgcacatc
     7981 aaaatacttt ggatgagaat tttgagggag aatcaataaa ctatactggt tggttagatt
     8041 tagatgaaaa gagatattat tttacagatg aatatattgc agcaactggt tcagttatta
     8101 ttgatggtga ggagtattat tttgatcctg atacagctca attagtgatt agtgaataga
     8161 taaaaatatg ttaaatatat cctcttatac ttaaatatat aaaaataaac aaaatgatac
     8221 actacataaa gtgttctatc taatatgaag atttaccaat aaaaaggtgg actatgatga
     8281 atgcacagta gttcaccttt ttatatttct aatggtaaca aaatattttt ttatataaac
     8341 ctaggaggcg ttatgaatat gacaatatct tttttatcag agcatatatt tataaagtta
     8401 gtaattttaa ctatatcatt tgatacatta ttaggatgtt taagtgcaat aaaaagtcgt
     8461 aaatttaatt ctagttttgg aatagatgga ggaatcagaa aagtagcaat gatagcatgt
     8521 atattttttt tatcagtagt tgacattctt acaaagttta actttttatt tatgttacca
     8581 caagattgta tcaatttttt aagactaaaa catcttggaa tatctgaatt tttctctatt
     8641 ttatttattt tatatgaaag tgtaagtata ttaaaaaata tgtgcttatg tggattacca
     8701 gtacctaaga gattaaagga aaaaatagca attttactag atgcaatgac agatgaaatg
     8761 aatgctaagg gtgaaaagta agtaatggta gatataataa agatattaac aaataaaaag
     8821 tgttatccaa ataagaatag ctgaaagtta tcataattca tgaaactaat aatgaaaacg
     8881 agggagcaga tgccaagaga cacacaagta ttaaatacat ataatttcga agcaagtgtt
     8941 cattactata tggatgacaa ggtagtatat caaacattgg ttcacaaaga tggtgcatgg
     9001 tcagttggta aaatctatta agctacatta gttacagata tcacaaacta taatagttaa
     9061 acatagaaat atgtgtaaat tgtgatggaa attattcaaa aacacaaaaa tacgtgatga
     9121 aggacaaaat gatatagaaa ataagtatca aaccttaata aatgatttaa ttgatagttt
     9181 aaaagttata ggaaaaatat ataaagaaat aaaaacatta aaaaaatata agatatgttt
     9241 acaaattact atcagacaat ctccttatct aatagaagag tcaattaact aattgagtat
     9301 ctttaaattg aaatgttagg aagtgattta aatatgaaaa cttaaattat aaaaaatcaa
     9361 tattaattta tttttaaaaa atagaaagga gtgtataaga tttattttca aagtttaaaa
     9421 acaagaaaat caatttaaat ttcagaagga ataaatgtgg ttatagaagt ggatttatta
     9481 tcaaaaataa taatactagg aggtttttat gtctttaata tctaaagaag agttaataaa
     9541 actcgcatat agcattagac caagagaaaa tgagtataaa actatactaa ctaatttaga
     9601 cgaatataat aagttaacta caaacaataa tgaaaataaa tatttacaat taaaaaaact
     9661 aaatgaatca attgatgttt ttatgaataa atataaaaat tcaagcagaa atagagcact
     9721 ctctaatcta aaaaaagata tattaaaaga agtaattctt attaaaaatt ccaatacaag
     9781 ccctgtagaa aaaaatttac attttgtatg gataggtgga gaagtcagtg atattgctct
     9841 tgaatacata aaacaatggg ctgatattaa tgcagaatat aatattaaac tgtggtatga
     9901 tagtgaagca ttcttagtca atacactaaa aaaggctata gttgaatctt ctaccactga
     9961 agcattacag ttactagagg aagagattca aaatcctcaa tttgataata tgaaatttta
    10021 caaaaaaagg atggaattta tatatgatag acaaaaaagg tttataaatt attataaatc
    10081 tcaaatcaat aaacctacag tacctacaat agatgatatt ataaagtctc atctagtatc
    10141 tgaatataat agagatgaaa ctttattaga atcatataga acaaattctt tgagaaaaat
    10201 aaatagtaat catgggatag atatcagagc taatagtttg tttacagaac aagagttatt
    10261 aaatatttat agtcaggagt tgttaaatcg tggaaattta gctgcagcat ctgacatagt
    10321 aagattatta gccctaaaaa attttggcgg agtatattta gatgttgata tgcttccagg
    10381 tattcactct gatttattta aaacaatatc tagacctagc tctattggac tagaccgttg
    10441 ggaaatgata aaattagagg ctattatgaa gtataaaaaa tatataaata attatacatc
    10501 agaaaacttt gataaacttg atcaacaatt aaaagataat tttaaactca ttatagaaag
    10561 taaaagtgaa aaatctgaga tattttctaa attagaaaat ttaaatgtat ctgatcttga
    10621 aattaaaata gctttcgctt taggcagtgt tataaatcaa gccttgatat caaaacaagg
    10681 ttcatatctt actaacctag taatagaaca aataaaaaat agataccaat ttttaaacca
    10741 acaccttaac ccagccatag agtctgataa taacttcaca gatactacta aaatttttca
    10801 tgattcatta tttaattcag ctaccgcaga aaactctatg tttttaacaa aaatagcacc
    10861 atacttacaa gtaggtttta tgccagaagc tcgctccaca ataagtttaa gtggtccagg
    10921 agcttatgca tcagcttact atgatttcat aaatttacaa gaaaatacta tagaaaaaac
    10981 tttaaaagca tcagatttaa tagaatttaa attcccagaa aataatctat ctcaattgac
    11041 agaacaagaa ataaatagtc tatggagctt tgatcaagca agtgcaaaat atcaatttga
    11101 gaaatatgta agagattata ctggtggatc tctttctgaa gacaatgggg tagactttaa
    11161 taaaaatact gcccttgata aaaactattt attaaataat aaaattccat caaacaatgt
    11221 agaagaagct ggaagtaaaa attatgttca ttatatcata cagttacaag gagatgatat
    11281 aagttatgaa gcaacatgca atttattttc taaaaatcct aaaaatagta ttattataca
    11341 acgaaatatg aatgaaagtg caaaaagtta ctttttaagt gatgatggag aatctatttt
    11401 agaattaaat aaatatagga tacctgaaag attaaaaaat aaggaaaaag taaaagtaac
    11461 ctttattgga catggtaaag atgaattcaa cacaagcgaa tttgctagat taagtgtaga
    11521 ttcactttcc aatgagataa gttcattttt agataccata aaattagata tatcacctaa
    11581 aaatgtagaa gtaaacttac ttggatgtaa tatgtttagt tatgatttta atgttgaaga
    11641 aacttatcct ggtaagttgc tattaagtat tatggacaaa attacttcca ctttacctga
    11701 tgtaaataaa aattctatta ctataggagc aaatcaatat gaagtaagaa ttaatagtga
    11761 gggaagaaaa gaacttctgg ctcactcagg taaatggata aataaagaag aagctattat
    11821 gagcgattta tctagtaaag aatacatttt ttttgattct atagataata agctaaaagc
    11881 aaagtccaag aatattccag gattagcatc aatatcagaa gatataaaaa cattattact
    11941 tgatgcaagt gttagtcctg atacaaaatt tattttaaat aatcttaagc ttaatattga
    12001 atcttctatt ggtgattaca tttattatga aaaattagag cctgttaaaa atataattca
    12061 caattctata gatgatttaa tagatgagtt caatctactt gaaaatgtat ctgatgaatt
    12121 atatgaatta aaaaaattaa ataatctaga tgagaagtat ttaatatctt ttgaagatat
    12181 ctcaaaaaat aattcaactt actctgtaag atttattaac aaaagtaatg gtgagtcagt
    12241 ttatgtagaa acagaaaaag aaattttttc aaaatatagc gaacatatta caaaagaaat
    12301 aagtactata aagaatagta taattacaga tgttaatggt aatttattgg ataatataca
    12361 gttagatcat acttctcaag ttaatacatt aaacgcagca ttctttattc aatcattaat
    12421 agattatagt agcaataaag atgtactgaa tgatttaagt acctcagtta aggttcaact
    12481 ttatgctcaa ctatttagta caggtttaaa tactatatat gactctatcc aattagtaaa
    12541 tttaatatca aatgcagtaa atgatactat aaatgtacta cctacaataa cagaggggat
    12601 acctattgta tctactatat tagacggaat aaacttaggt gcagcaatta aggaattact
    12661 agacgaacat gacccattac taaaaaaaga actagaagct aaggtgggtg ttttagcaat
    12721 aaatatgtca ttatctatag ctgcaactgt agcttcaatt gttggaatag gtgctgaagt
    12781 tactattttc ttattaccta tagctggtat atctgcagga ataccttcat tagttaataa
    12841 tgaattaata ttgcatgata aggcaacttc agtggtaaac tattttaatc atttgtctga
    12901 atctaaaaaa tatggccctc ttaaaacaga agatgataaa attttagttc ctattgatga
    12961 tttagtaata tcagaaatag attttaataa taattcgata aaactaggaa catgtaatat
    13021 attagcaatg gaggggggat caggacacac agtgactagt aatatagatc actttttctc
    13081 atctccatct ataagttctc atattccttc attatcaatt tattctgcaa taggtataga
    13141 aacagaaaat ctagattttt caaaaaaaat aatgatgtta cctaatgctc cttcaagagt
    13201 gttttggtgg gaaactggag cagttccagg tttaaggtca ttggaaaatg atggaactag
    13261 attacttgat tcaataagag atttataccc aggcaaattt tactggagat tctatgcttt
    13321 tttcgattat gcaataacta cattaaaacc agtttatgaa gacactaata ttaaaattaa
    13381 actagataaa gatactagaa acttcataat gccaactata actactaacg aaattagaaa
    13441 caaattatct tattcatttg atggagcagg aggaacttac tctttattat tatcttcata
    13501 tccaatatca acgaatataa atttatctaa agatgattta tggatattta atattgataa
    13561 tgaagtaaga gaaatatcta tagaaaatgg tactattaaa aaaggaaagt taataaaaga
    13621 tgttttaagt aaaattgata taaataaaaa taaacttatt ataggtaatc aaacaataga
    13681 tttttcaggc gatatagata ataaagatag atatatattc ttgacttgtg agttagatga
    13741 taaaattagt ttaataatag aaataaatct tgttgcaaaa tcttatagtt tgttattgtc
    13801 tggggataaa aattatttga tatccaattt atctaatact attgagaaaa tcaatacttt
    13861 aggcctagat agtaaaaata tagcgtacaa ttacactgat gaatctaata ataaatattt
    13921 tggagctata tctaaaacaa gtcaaaaaag cataatacat tataaaaaag acagtaaaaa
    13981 tatattagaa ttttataatg acagtacatt agaatttaac agtaaagatt ttattgctga
    14041 agatataaat gtatttatga a
//
LOCUS       DAEMHW010000100        13930 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEMHW010000100 DAEMHW010000000
VERSION     DAEMHW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13930
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.100"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..309)
                     /locus_tag="KPK67_002380"
     CDS             complement(<1..309)
                     /locus_tag="KPK67_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG2459337.1"
                     /translation="MNSKKEDIYELNFGQLIEEKTGIIEENQNVFNAKVNVAVSIGNI
                     TETIKNILDLKEGSIIKLNKNVDEKLDIYANDRLFAYGESIITNETLSVRLSKIEDSD
                     V"
     gene            complement(333..761)
                     /locus_tag="KPK67_002381"
     CDS             complement(333..761)
                     /locus_tag="KPK67_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBG2459338.1"
                     /translation="MGINIDKLNISPSRSIEVKKTKENNKFSDSLNFVKQSSSLEDIK
                     RNLDGVKKAGERLVLTQNYGDISRYKNAVKEYLKSVVDNMYSLDKSSSFWEHQYYKNV
                     KIIDNKLEDMTSKLLSEEKENIDIVSTVDMIQGLLIDMYR"
     gene            complement(770..1438)
                     /locus_tag="KPK67_002382"
     CDS             complement(770..1438)
                     /locus_tag="KPK67_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBG2459339.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCGYNNSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSEKLFNELDSVANNTVGKFIEVDTKDKSVKSRIENAVEKA
                     SKKYNVDANLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTNPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMRSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            complement(1884..6965)
                     /locus_tag="KPK67_002383"
                     /pseudo
     CDS             complement(1884..6965)
                     /locus_tag="KPK67_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
     gene            complement(6976..8508)
                     /gene="purH"
                     /locus_tag="KPK67_002384"
     CDS             complement(6976..8508)
                     /gene="purH"
                     /locus_tag="KPK67_002384"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBG2459340.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAVVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            complement(8501..9094)
                     /locus_tag="KPK67_002385"
     CDS             complement(8501..9094)
                     /locus_tag="KPK67_002385"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBG2459341.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            complement(9088..10164)
                     /locus_tag="KPK67_002386"
     CDS             complement(9088..10164)
                     /locus_tag="KPK67_002386"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBG2459342.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            complement(10158..11525)
                     /gene="purF"
                     /locus_tag="KPK67_002387"
     CDS             complement(10158..11525)
                     /gene="purF"
                     /locus_tag="KPK67_002387"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBG2459343.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            complement(11537..12235)
                     /locus_tag="KPK67_002388"
     CDS             complement(11537..12235)
                     /locus_tag="KPK67_002388"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG2459344.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            complement(12241..12717)
                     /gene="purE"
                     /locus_tag="KPK67_002389"
     CDS             complement(12241..12717)
                     /gene="purE"
                     /locus_tag="KPK67_002389"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBG2459345.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            13167..13679
                     /locus_tag="KPK67_002390"
     CDS             13167..13679
                     /locus_tag="KPK67_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2459346.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     RIHRNVYKNR"
BASE COUNT         4157 a         2654 c         1480 g         5639 t
ORIGIN      
        1 tacgtctgaa tcttctattt tagataatct tacagataat gtttcatttg taataatact
       61 ttctccataa gcaaacaatc tgtcatttgc atatatgtct aatttttcat ctacattctt
      121 atttagtttt attatagaac cttcttttaa atctaatata ttttttattg tttctgttat
      181 gtttcctata gaaacagcca catttacttt agcattaaaa acattttgat tttcctctat
      241 aatcccagtc ttttcttcta ttaattgacc aaaattcaat tcatatatat cttctttttt
      301 gctattcata aaactattct ccttatgtaa tcttatctat acatatctat caataagcct
      361 tgtatcatat caacagtaga aacaatatct atattttctt tttcttcact aagtagttta
      421 ctagtcatat cctcaagttt gttatcaata atctttacat ttttataata ttgatgctcc
      481 cagaaactac tacttttatc aagtgaatac atattatcta caacagattt taggtattct
      541 tttactgcat ttttatatct actaatgtca ccatagtttt gtgtaagtac aagcctctcc
      601 ccagcctttt ttacaccatc taaattcctt tttatatctt ctagtgaaga agattgtttt
      661 acaaagttta agctgtcaga aaacttatta ttttcctttg tctttttaac ttctatactt
      721 ctacttggac ttatatttag cttgtctata tttataccca tagtaaatcc taccctctat
      781 aatatttcat aactttaggt atataatttt gtgtctcctt tggcatttta tacaagtcag
      841 aaattgattc tacgcctcta cttctcattc taccaggtcc accattatat gccatcaatc
      901 ccatttctat gctacctcca aacatatcta tatattcctt aatatgtcta gtaccagcat
      961 ctatattttg ttctatgtta aaagggtttg ttactcccaa atctctgcaa ttttctggca
     1021 taagttgcat aagacccttg gcaccagcag aagatacagt atttgggtta aaatctgatt
     1081 caaccttaat tattgccttt ataagatttg catctacatt atatttcttt gatgctttct
     1141 ctacagcatt ttcaattctt gactttacac ttttatcttt tgtatcaact tcaataaact
     1201 taccaactgt attatttgct acactatcaa gttcattaaa cagtttttca gaattagatg
     1261 ataaattata tccattagag gtttgttgat tattttgtga taatgctttc aacaatgtca
     1321 tcataatcat atcaaatcca gacgaattat tatatccaca gttgcattga tttgtggtat
     1381 tactaccaga taattgggtc aatgccagta tatttattag tgaacttatt tcgttcaaaa
     1441 taaaactccc ctttctaaat tttataaact ttaaagttat ccttatataa ttcaagatac
     1501 ttgagttcct ataaaatctt cttgttatag actactttgt taaattaatt tcttttatat
     1561 tcattaaaac tttagattta gtttcactac attatgatta tcggataaat aaaaaaaata
     1621 tttatactaa aattatttac taattatttt tttaattgac taccataccc ttttattaat
     1681 actttccttt attatgatta ctattaataa ataatcaact ttaattaatt ataacacagt
     1741 atattttaat ttaagctgaa ctattatttc ttatataaat aaaaaagaga tgtaatatta
     1801 aatgattatc atcacattat tacatctctt caaatctaaa tatctaaaat atatattcta
     1861 gctatcaatt aaacatcaaa gttttatcta aaatacttaa ctccagattc aaatatcttc
     1921 tgttcttttt cccctatgat attcttatat acttcctcac caattctttc agaatgtccc
     1981 attttaccaa acactctacc atcagcacta gttatacctt caacagcata gaatgaacca
     2041 tttggattaa actcaatatc ataagtagct tcattattaa agtcaacata ttgagttgct
     2101 acttgtccat tagcaattaa ttctctcata acatcttcac tggcaacaaa cttaccttct
     2161 ccatgagaaa ttgctatatt atgaatatct ccaacattag tttgagctaa ccaaggagat
     2221 ttattagatg aaattctagt tctagcaatc ttagcttgat ggcgaccaat attattgtaa
     2281 gtaagtgttg gagcactttc acttggaact cttatttctc catatggaac taaccctaat
     2341 ttaattaata cttggaatcc attacagata cctaacatta gtccatcttt ttgagttaaa
     2401 aattcattta tagcttcttg cactctagga tttctaaata cagtagctat aaatttacca
     2461 gaaccatctg gctcatcacc agcactaaat ccacctggta acattattat ttgagaagat
     2521 tttatttcgt ttactattgt atctatagaa tcttctatgt ctttataagt taaattctta
     2581 aatactctta tacttgcatt agctcctgct ctttcaaatg ctcttgcaga gtcatattca
     2641 cagtttgttc cagggaatgc tggtataaat actcttggag tagctatact taatgatgat
     2701 tttttagctt ctccttgtga tatataactt atagtttcta tctttgattt tacttcttct
     2761 gttttagttg ggaatatagg ctctaaagta ctacaatgtg ctttatataa ttcatctaat
     2821 gcaagttctt cacctttgat aaatatacta gcttctttaa ctgtacttcc aagcacaaca
     2881 taattgtatc cttctaacat acttaaatca tcatttgcta attcaagtac tatatttcca
     2941 taactagcct caaataattt atcatctgta aatgcttttt cagcttcttc actaaactta
     3001 aatcctattt tattaccaaa agccatttta cttattgctt cacctacacc tgcaaatcct
     3061 aatgcatatg ttgataaaac tttatttcca tgtataagtt ctcttacttt atctaagttt
     3121 ctctttaatt cctcaaaatc tacaacatca ttttctgcct tgtttacaca taacattact
     3181 acttttgagt ctgctttttt aaattctgga gatactactt tcttcgcatc tactgtgtca
     3241 acagcaaatg aaaccaatgt tggtggaact tcaatatctt taaatgtacc agacatactg
     3301 tcttttccac ctatagcagg tatctcaaat ttactttgtg cataaaatgc tcctaaaagt
     3361 gctgagaatg gtttacccca tttttctgga ttgttaccaa gtttttcaaa atactcttgg
     3421 aatgtaagtc ttgttgtact ataatttcct ccaatagcaa ccaatttaca cacagattct
     3481 actactgcat acaaggcacc atggaatggg ctccattttc ctacctttgg attgtatcca
     3541 taagtcatta ttgtagaagt attagtttct cctccaagta ctggtatctt agcaaccata
     3601 ccttgtgttg gagtcgcttg atactttcct ccaaatggca taagtactgt attaccacca
     3661 attgtattat caaacatctc tactaatcct ttttgagaac aaacattaag gtcagatagt
     3721 acttttgtaa acttttcttt catacttgca cacttaacat cttttactat ttcattaaag
     3781 ctaaagaatg tattttcttc atctacttta gtaacatgaa catcagtagt ttgttttact
     3841 ccgtttgtat ctaaaaactc tctatttata tctacaattg cttttccatt ccagaacatt
     3901 cttaaatatc ctgtatctgt tactgtagct acatgtgtag cttctaagtt ttcttctact
     3961 gctaattgta taaatttatc tttattttct ttttttatgg ctactgccat acgctcttgt
     4021 gactctgaaa ttgcaagctc agttccatca agcccatcat attttttagg aactaagtct
     4081 aagtttatat ctaagctatc agaaatctct cctatagcaa cacaaactcc acctgctcca
     4141 aaatcattac atctttttat catttgagca acttctttat ttctaaagaa tctttgtatt
     4201 tttctttcgt taggagcatc tcctttttga acttctgcac tacaagttaa gattgactct
     4261 tcactatgtt ctttagaaga accagttgct cctccacaac catctcttcc tgtttttcct
     4321 ccaagaagaa cgattacatc tcctgcttca ggtctttctc ttactacatt ctcttttggt
     4381 gctgctgcta taactgcacc tatttccatt ctctttgcaa caaagttttc atcatatact
     4441 tctgcaactt gtccagttgt aagacctatt tggttaccat atgaactata tccatgagca
     4501 gcttctgtag ttatttttct ttgcataagt tttccaggta aagtatcttc taaagtcgtt
     4561 cttgggtcag cacttcctgt aacacgcatt gcttgataaa cataacttct tcctgataat
     4621 gggtctctta tagctcctcc taaacaagta gctgctccac caaatggttc tatttcagtt
     4681 ggatggttgt gagtttcatt tttaaacatt actagatatt tttccatctt accatcaact
     4741 tcaacatcta cgtttatact acatgcattt atttcttcac tttcatctag gtcatttaat
     4801 ttcccatttt tctttaattc ttttactgca acagtggcta tatccattaa acatatatct
     4861 ttatgtctgt tcacatatac attgtctctt gagttaagat acatttgata cgcttctttt
     4921 actatatcat taaacttacc atcttctatc tttacatttt ctatttcagt cataaaagta
     4981 gtatgtctac aatgatcaga ccaataagta tctaatactt ttatttctgt tatagttgga
     5041 tttctctttt ctgtatcttt aaagtatttt tgaacgtgtt gtaagtctcc taaagtcatt
     5101 gctaatcctt tttcacttac aaatgttctt aatccatttt catctaaatc tataaatcca
     5161 tctaatactt caactgttgt tggtatttca gtttccattt ttaacgtttc tggtttctct
     5221 aaagatgctt ctctactatc tactggattt atacagtaat cttttatttt agaaaactct
     5281 tcatcagtta ttttgcctga taatatataa acttttgctg tattaatagc aggtcttatc
     5341 ccttgattta caatttgtac acattgagct gcccaatcac ctctttggtc gtattgacca
     5401 ggaagaaatt ctattgcaaa tactctttca tcatttaatt ttggtatttc ttcataatat
     5461 accacatcta aatttggttc agagaatatt gtttttgctg cattttcata aacttcttct
     5521 gatattcctt ctacatcata tctatttaat attcttaaat tttctatatt atctatgtgt
     5581 aaactttcta ctaagtcttt ttttaaagct ttagcctcaa ggtcaaaacc ttctcttttt
     5641 tcaactaata ctcttctaac cattgagtct ttattttcag tattcaacat accttatcct
     5701 ccattttaga aaaattatct tcctatgtct gttctatagt gcattccttc aaagtttatc
     5761 ttctttatgt tttcataaac tttttctgct gcatccttaa ctgtagttga ctttgcagtt
     5821 ataccaatta ctcttcctcc attggtaacc aaattaccat caaacatctt agtgccacta
     5881 tggaatacta ctatatcatc atccaagctt tcaagaccag ttataatttt ccctttttca
     5941 taactgtctg gataccctcc tgatgtaagc ataacacata cagcttcatc atcactatag
     6001 tttatttcta tatcttttaa cttattatct agtatagctt ccattatctc atgtaaatct
     6061 gtttctaatc taaataaaac agattgtgtc tcagggtctc caaatcttac attatattca
     6121 agtacttttt ctccatcttc agttatcata agaccaacaa ataaaatacc tttaaaatta
     6181 agtccatctt ttttgaagcc ttctaaagtt ttttctaata ctgtctcttt tactttattt
     6241 gctaattctt ctgtatatat ttcactaggt gaaaaagttc ccattccccc agtgtttgga
     6301 ccttgctcat agttgtttac ttttttatgg tcttttgcac ttgccatagg aactattgta
     6361 tcattatcta caaatgctag aattgaagtt tctattcctt ttaaaaactc ttcaattact
     6421 attttatctc cagcagctcc aaacttctta tctgacatca tttcttttaa agttgctata
     6481 gcatcttctc tattttcagg tattacaaca ccttttccag cagctaatcc gtccgctttt
     6541 attactactg gatatccaaa actatctatt tctgatatag cttcttctaa gtttgtatat
     6601 tctttatatt ttgcagtagg tatgttatgt tttatcataa attcttttga aaaagcttta
     6661 cttccttcaa gttgagcaca ttttttatta ggtccaaata ctcttaatcc ttctttttca
     6721 aactcatcta ccattcccat aactagtggt acttctggtc ctactattgt cagacctatt
     6781 tcattttctt tggcaaattt taaaagttct tctatattag tatcacctat atttacacat
     6841 tctgctacgt ttgcaattcc tgcattacct ggtgcacaat aaattttttc tacatttttt
     6901 tctttgctta gtttccagca tatagcatgc tctcttccgc caccgcctac aaccaatatc
     6961 ttcataatat cctccttagt gtttgaagtg tcttattcct gtaaatacca taaccatatc
     7021 atacttgtca caagcttcta ttgaatcttt atcttttata gagccacctg gttgaactac
     7081 tgctgatatt ccatattcat gagctaatgt tacacaatca tcaaatggga aaaatgcgtc
     7141 tgacgctaaa acagctcctg taaaatcttt atctttgtta ttttcaagag cattttttaa
     7201 tgcccaaatt cttgatgttt gtccacaacc taaagctaga gtttgtccat tttttactac
     7261 tgcaattgca tttgatttca tattttttac tactctcata ccaaattcca tgtccttaag
     7321 ttgtgcatct gttggttttt cctttgttac attttcactt ttttctgcta atatattatt
     7381 tctatcttgt atcaataatt ttccatctaa atatttcatt tcatatggtt gtaaactatt
     7441 ttctatttta gctaatttta atattcttag atttttcttt tgtttaagta tctctaaagc
     7501 ttctggttca aaatcatatg ctactactat ttctaagaat atttcattta atttctctgc
     7561 tgtagcttta tctatagtag aagttattcc tactattcct ccaaatattg aaaccttatc
     7621 cgcttcataa cattttgtat atgcttcaaa agcatctttt cctaaaccta ccccacatga
     7681 attagcatgt tttatagcta ctgaaactac ttcttcacta tctttaaatt ctctcattac
     7741 ttctaaacat ccatgtaaat catttaagtt gttaaaagaa agttctttac cgcctaattg
     7801 ctcataattt aagattggat tttttgcatt tgactgtgaa tataagaaac cattttgatg
     7861 tggattttct ccatatctta aagtttgttc tttttggaaa gttaaattta atatgtcagg
     7921 atacgtatct ccaacttctc cagcaaaata actagatatt aacgcatcat atcttcctgt
     7981 agttgagaaa gctttatatg ataatttttt tctgtcttct aatgtcattg tatctgtttt
     8041 taatttttct attatagaat cataatcttt tatatctact actactatta catccttata
     8101 gtttttggca gcagaacgta tcatagatgg tccaccaata tctatatttt caattatttc
     8161 atcatgactt tttccagctt ttaaagttcc ttcaaaatca tatagattta cagctactaa
     8221 atctattgaa tgtattttgt gttcatttac agtttctaca tgagattctt tatctctttt
     8281 ataaagtata ccaccatgta tatatggatt taatgttttt actcttccat ctaatatttc
     8341 tggaaaattt gtaacatctt caacttgcat tacattaaca ccattttctt ttaatgtttt
     8401 aaatgtattt cctgtagata taatttcata atctaattta tttaactctt tagcaaattc
     8461 cactacacca gtcttgtcag ttacacttat taaagctctc ttactcattt ataaacactc
     8521 tcctaccttg aagttttaat ttattttcac aaaaaagact tatactttct tttaaaatcc
     8581 tatgttcaac ttctaacact ctttttgcaa gtgtctttgc atcatcatct tgatttacct
     8641 taactacatc ttgcattatg attggtccag tatcagcacc ttcatctaca aaatgaactg
     8701 tagcaccagt tactttagct ccgtaatcta taacaccttg atgaactttt tctccataga
     8761 atccagctcc acaaaatgaa ggaattaatg atggatgtat gtttatcatt ttgtttctaa
     8821 actcattaac cagctttggg ctgattattt ttaaataacc agccaaaact actaaatcta
     8881 ttttattttc cttaagaatt tctattatct tgtcttcatc agtttcacaa attgctttta
     8941 tgttatgatt tttagctctc tctagcccat aagcaccttg cttacttgag ataactactt
     9001 taacctgacc ttttatttct ccagactcag taccatctat aactgcttgt aaatttgttc
     9061 ccccaccaga tattaaaact cctatattta gcataattct acaccttcat gactatctac
     9121 tacttcacca attatataag ctttatcttc taataattca ctatattttt tatctacata
     9181 atcagcattg tcattttctc tattatttat aaagtttaca acatcatcag cattttcttt
     9241 atctactatt aaaactagcc caatacccat gttaaaactc ttatgaagtt ctctctcatc
     9301 aacagcatta aaaccttcta tcattttaaa tataggaggc ttctcccaag attttttatt
     9361 gatatctagt cctaatcctt taggtataac tcttgttata ttttctataa gccctccacc
     9421 agttatgtga gctatagctt ttatgtcatg tttagctaat acatctaaag ctaatttaac
     9481 atatatttta gttggtgtta aaagtgcttc tccaactgtc attcctagtt cttcaacata
     9541 ttgttccatt ttataattgt aagtttctaa aaatatcttt cttataaaag aaaatccatt
     9601 gctgtgtact ccacttgaag atataccaac caatacatct cctgatttaa cattgtttcc
     9661 agaaacaatc ttttctttgt ctgctattcc tacagaaaat cctgctaaat catagtcatc
     9721 ttctccatac attccaggca tttcagcagt ttctccacca attagtgcac aaccagacat
     9781 tttacaacca tctgcaattc caccaactat tttttcgata tgctcaggca ctaatttccc
     9841 aagagctata tagtcaagga agaataatgg ttttgctcct tgacaaatta aatcattcac
     9901 gcacattgca actaaatcta ttccaacagt atcatgcttg tccatcatct gagctatttt
     9961 taacttagtc ccaacaccat ctgtagatgc aagcaaaact ggttctttca tacctacaaa
    10021 atcttttaaa ctgtataatc cactgaagtt acctaaatct ccaactacat ttccatcata
    10081 agttcctttt attttacctt ttataaggtc tactgctcta tttccttcat ctatatctac
    10141 acctgattct ttatatgtta acatgaaagt agttcctcct tatcaatttt cttaactggg
    10201 tattctccat caaaacaagc tttacagaaa gtagacttat gttctgttgc atctaacatt
    10261 ccttctatat ctaggaattt taaactatca catccaatat attctctcat ttcttctacg
    10321 ttgttacttg atgcaattaa tttagagcga tttggagtat ctattccata gtaacaagag
    10381 taagctactg gaggagaagt tattctaaga tgaatttctt ttgctcctgc ttctcttaaa
    10441 gattttacta attgtttaga tgtagttcct cttacaattg agtcgtctac taaaattata
    10501 gattttcctt ttattattgt tgaaagagga tttaatttta tttttactgc tatttctctt
    10561 tcttcttgtg taggttttat aaatgttctt cctacatatc tatttttaac taagccttca
    10621 cttattttta atccactagc atttgcataa ccaatagctc ctggccaacc tgaatctgga
    10681 actggaacta ccatatctgc ctttacagtt tcattctgag ctaatctctc accacattta
    10741 attctaaatt catatgcatt tacattgtct atagttgcat catttcttgc aaagtatatg
    10801 tgttcaaata tacaactttt cttaactggt ttataatttt ctgaataaaa atatgatttt
    10861 agttctccat ctttgactac tattatctct cctggctcta catctcttat aacttctccg
    10921 ccaagtatat ctattgcaca attttctgat gcaaatatat attcatcacc ctttttacca
    10981 agtacaagag gtctgaatcc atgtgggtct ctaactgcaa ccaattcttc ttgactcatt
    11041 attacaagag catatgcacc ttttatataa tccattgtca cttttaaact ttctactata
    11101 tcccctttgt aatatctagc aagtatatat aaaatcactt ctgtatctga attagtttgg
    11161 aacatatatc catcttcttc tagcatatct tttaaataat ttgcattaac tagatttcca
    11221 ttatgagcta tggctaattg tctttttcta caacttccaa ctaaaggttg acagttagaa
    11281 acatgacttc ctccagctgt tgaatatcta acatgtccaa tccctatatt tccttttaac
    11341 ttagataatt cattttcctt aaaaacatct ccaactaatc ccatatcttt tttgtactta
    11401 atttctccat catctaaaag agctaaacca cagctttctt gccccctgtg ttgcatagaa
    11461 tataaagaat aatatagttc tttagttaca tctttattag agtaaatccc taaaactcca
    11521 cacatacatt tgcctcctat ttattattca ttcttgaaag tacttccgta taaccttcta
    11581 caaggtctcc aaggtctttt ctaaatctgt ccttatctaa cttttcattt gtatttacat
    11641 cccaaagtct acaagtgtct ggtgatactt catctgccaa tattatattt ccttcagaat
    11701 ctttaccaaa ttctatttta aagtcaacta actttaggtt taattttaag aaaaatgctt
    11761 ttagtaactc gtttattttt aaagtttctt ctcttaagaa ttttaactct tctctagttg
    11821 ctaacttcat tgccactgca taatcatcat ttagcattgg gtcaccataa gcatcatttt
    11881 tgtagcttat ttcaaaaatt ggctcatcga aaactacacc ttcttcaaga ccaactcttt
    11941 tacagataga accagcagca atatttctta ctattacttc taatggtaaa atctctactt
    12001 ttttaactaa catttctcta tctgataaac tttttataaa gtgagtattt atattatttt
    12061 cttttaacat ttcaaagatt attgttgata tcttattatt tagtatacct tttgaagata
    12121 tttctgcttt tttttctcca ttaaatgctg ttgcatcatc tttataataa acaacatatt
    12181 cattttcatt atctgtagaa tatacttgtt ttgcttttcc ttcatataat aacatttctg
    12241 ctaccccctt aaattcttgt cgtcttctaa caccttttgt gccatctctt ctctatatga
    12301 tttcaaatcc tcttttaatt ttgggtattt taaagtcatt atttgtaatg ccaataaagc
    12361 tgcgtttaaa cctaagtcta ttgtaactgt tgcaactggg attccttttg gcatttgaac
    12421 tattgataaa agtgagtcta gtccatccat agttgatgat ttaataggta atcctattac
    12481 aggaattaat gtttgtgaag ctattacacc tggtaagtgt gctgcttttc ctgctgctgc
    12541 tattattaca tcagtatcat cttctatttc ttttaggaat attgaaagtt gttctggtgt
    12601 tctatgagct gataatgctc ttgctacaac ttctattcca tatttttcaa gcagttttat
    12661 cccttcttct aattttggat aatctgattt tgaacccata actactgcta ctttcattag
    12721 acttcccctc tccgtttata agttatttta cactacaaat cattcgtatt attatacgaa
    12781 ttatacacct taaattttat attaatatac atatattatc aactcttttt tgaaattttt
    12841 taaaaaaaat atttcttgta attttttcat tcgctacgta tcgaacttta tttatgtttt
    12901 tttgttaaaa taaaaaacag attatctcaa aattggtttt ctaattaaat tttaagccca
    12961 atactctttt ttaaatcttg tatattattt tttattactt cttttttaat taatactttg
    13021 gatttatagt cttgtttttt agttctgaat gttactttta cttagataaa accaacacaa
    13081 agtttattct ttatataagt aatagctaaa atttaaagta atgatattat ataaatagaa
    13141 ataattttat ttaaaggaga atctgtatgt tagaagttat aaaaaataga cgttcaataa
    13201 gaacatatat agataaaaat atagaagaag ataaaataac tgaaatacta aaatcagcta
    13261 tgcaagcacc ttcatcaaaa aatgctcaac cttgggaatt tattatagta gatgataagg
    13321 aactgctgaa acagctctca aagtcacagc atagagcaaa gcatatagaa tttgcaccat
    13381 tatgtatagt agtgcttgga aatagagata aattcttaaa acctggtaaa tggatacaag
    13441 atttaggtgc ttgcacacaa aatttattat tagaagttac taatcaaggt ctagctgctt
    13501 gttgggcagg agtatttcct aaaaataaag tggtaaataa agttagacaa actttagact
    13561 taccattaaa attagttcct tatgcactta tatctattgg atatagtgaa gaaaaaaatg
    13621 agtttataga ccgatttgat gaaaatagaa tacatagaaa tgtatataaa aatagataat
    13681 gtttagtaat ataaatgaaa atacatagta aaaatgcctt tagaatacat ttgtattcta
    13741 aaggcattaa ctttgactaa acacctaatt agcctctaag gtaaaattta actatcattt
    13801 attatttata taccaattga atattatatc cttcaatatt tttatttatt tcaaatatat
    13861 taagaatatc ctacaattaa agttagctat ttttcattaa agtattttag taaattaata
    13921 atgataatca
//
LOCUS       DAEMHW010000101        13639 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEMHW010000101 DAEMHW010000000
VERSION     DAEMHW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.101"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            802..957
                     /locus_tag="KPK67_002391"
     CDS             802..957
                     /locus_tag="KPK67_002391"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459347.1"
                     /translation="MDEERKKLIDDAEKLANIGINQILKFRDSLDIENIKYINIVATN
                     LKPNIKK"
     gene            complement(976..1332)
                     /locus_tag="KPK67_002392"
     CDS             complement(976..1332)
                     /locus_tag="KPK67_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2459348.1"
                     /translation="MHERIYKIRTSLKMSQREFGEKLGVSRDVISNIEYNRVTPKPVF
                     IQHLCELYNVNKEWLLTGEGEMFIEFSENEKVLKIATDIINEDDKFMKNILFTFSNLN
                     DEQKEFLINLMKNMTQ"
     gene            1495..1680
                     /locus_tag="KPK67_002393"
     CDS             1495..1680
                     /locus_tag="KPK67_002393"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459349.1"
                     /translation="MKTQKTTSHLNNLNLDDDDRKLIQMTLLFKKLNLEQQNYISAMA
                     DGMKVSNELLEKSTMNN"
     gene            1800..2552
                     /locus_tag="KPK67_002394"
     CDS             1800..2552
                     /locus_tag="KPK67_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HBG2459350.1"
                     /translation="MSNNLQIFKNNDFGEIRTVEIDGKPYFVATDIARCLGYKDTTNA
                     IKQHCKWVVKHHIPHPQSKTKTLEVNVIPEGDMYRLITNSELPSAEKFEHWVFDEVLP
                     SIRKTGTYSTKSKDESEIKYMNAQARLKNARVREAKIYLELADKVSQVGIPEFAQVMY
                     SKTTELLSGETLIPLPKIDKKTYSATEVGKMLGISANKVGGLANAYNLKTEEYGIKVW
                     DKAKYSDKQVPNFRYYENVIPVLEKALKSFEN"
     gene            2610..3278
                     /locus_tag="KPK67_002395"
     CDS             2610..3278
                     /locus_tag="KPK67_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459351.1"
                     /translation="MNIKNLRRNKKMKENNRIVSAINEDIVGVTVDGEIDLTDKELEM
                     VNNFKKVMNSINNKIGINCGISLSDKDIEDNKNILDKIASKVAWHYKKMNFEHLEMDE
                     KLFDDFTFEFMLKFFGDMEEYELIKYLTYIGEESNELSDRERIFYKIVDTLDTIADIK
                     ISDQNKIEKEVGMCIGEDNYITYCIDEDLIKFYIKDEEELVINKNSPLLYMLDTLFYE
                     VHEE"
     gene            3314..3565
                     /locus_tag="KPK67_002396"
     CDS             3314..3565
                     /locus_tag="KPK67_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459352.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDEEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWKEFDEVAKEVAINIAMKKAETVES"
     gene            3696..3857
                     /locus_tag="KPK67_002397"
     CDS             3696..3857
                     /locus_tag="KPK67_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459353.1"
                     /translation="MLSLDISKKNVMTLKKDGKFLADIVFKDIKTGKKISVGTLNKKS
                     AGQSNQHNK"
     gene            3901..4074
                     /locus_tag="KPK67_002398"
     CDS             3901..4074
                     /locus_tag="KPK67_002398"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459354.1"
                     /translation="MVEVNIKIKGEASEIIDSIRAIFNSFGEDSMTEIKAEQEYKSRR
                     SRKLKTEDLSDQE"
     gene            4165..5343
                     /locus_tag="KPK67_002399"
     CDS             4165..5343
                     /locus_tag="KPK67_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG2459355.1"
                     /translation="MGLGKTFVGSEKLINLGSNTNLLICQKSKIDDWVHHFKTYYPDM
                     GIINGTKKLSEGIEYFKIVEVPFIVVINYELAFRRKELLDLKDFTLMLDESSMIQNEK
                     AKRSKFVLSLNPKNVILLSGTPCSGKYEQLYSQIKLLGWNISKDLYWKQYIDVEYKDI
                     GGFPMKVVTGYKNVDRLKKKLRDYGAVFMMSDEVFDLPKQIDNVMKISTTREYRKFKK
                     DSIISISDDIELVGDTTLTKMLYERQLCSQYNKFKLEAFGDLINSTSDRLIVFYNFNE
                     ELDRLIELSRDRPISIVNGSIKDLSNYEEYNNSITFVQYQAGAMGLNLQKCNKIVYFS
                     LTLSCELFMQSKKRVHRIGQENTCFYYYMICRNSVEENIYKSLQSGVDYTNNLFERGE
                     "
     gene            5347..5649
                     /locus_tag="KPK67_002400"
     CDS             5347..5649
                     /locus_tag="KPK67_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBG2459356.1"
                     /translation="MGAEKQFENKVKKFLKEQGCWFIKYWGGSAYTKSGIPDLLVCCS
                     GKFIGIEVKGEKGKASELQKYNIKEIEKAGGIGIILYPKDFDRFKKMILEIKKEGE"
     gene            5650..7335
                     /locus_tag="KPK67_002401"
     CDS             5650..7335
                     /locus_tag="KPK67_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2459357.1"
                     /translation="MQFSHSRIGVFKSCPYKYKLQYIDKVKTILNADSNNALILGTSL
                     HTGIEKDIDAAVKEYYFSYPSITDLHINEVMKLDYLIPKVKEILPKGFNEVQITTSDF
                     IGFIDLLAPHVYMTDTNLNTNETTIYYEDEERVFDIYDFKYSNNIDRYLESEQLHLYK
                     YFYEKQHTNHEIKDLYYVFVPKIQIRQKKTENLHQFRQRLLNELDKSEIKIVKVEYDP
                     NKVINFLVDIKRCLENKEFEKNQNKLCNWCDYQEYCEKGIDYMLLPNSERRSVEGINK
                     KTLWIYGAPFSGKTTLANDFEKPLMLNTDGNIKFVDAPYISIKDIVTVEGRMTKRQLA
                     WDVFKEAIRELEKKENDFETIIVDLLEDTYEACRLYMYDKMGITHESDDSFRAWDKVR
                     TEFLSTIKRLMNLDYKNIILISHEDTSKDITKKGGDKITAIKPNLQEKAANKIAGMVD
                     IVARVVADGDVRTLSFKTNEVIFGGGRLKVKSNEIPLDYNELMKVYDDISIEKKDVKK
                     SSKTKKSNAEEEVEMTPTVEETSEKTEKEQTEEEVIEQEEAIKPVKKTRKKRS"
     gene            7387..7830
                     /locus_tag="KPK67_002402"
     CDS             7387..7830
                     /locus_tag="KPK67_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003355843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HBG2459358.1"
                     /translation="MDFSKFDKAIDVKGLKEDIKEASENSGKFKDVPHGTYEVEINKM
                     ELSESKKGDPMFVCWFKILEGDYKDSLIFMNQVVKQGFQIHIVNEFLRSLETDIEVEF
                     ESYSQYAQLIMDIAEEIDGELEFAIEYGEKKGFNTFTIKDIFEVA"
     gene            8044..9777
                     /locus_tag="KPK67_002403"
     CDS             8044..9777
                     /locus_tag="KPK67_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459359.1"
                     /translation="MIFYDFEVFSYDWLVVFIDVLNKKEEVIVNDIDKLNSFYIEHRE
                     DIFIGYNSRHYDQYIFKGLLCGFNAKEINDYIIVKGQPGWKFSNLLRNIQINNYDVMT
                     SFHGLKQLEGFQGHSIKESNVSFNIDRPLTNDEIEETIKYCRYDVEQTIDVFIERKSE
                     FEAHMGLIKAFKLPVAYIGKTQTQLTAIILEATKKEHDDEFDLQIPDTLKIEKYKEVL
                     SWYKNPLNHDYSKNLKINISNVPHVFAWGGVHGAITKYYGEGYFLHVDVNSFYPSLMI
                     RYNYHSRNIKNPQKYVEIYDKNLQLKKEKSSLRPAYKLAVNKTYGGMKDKNNNLYDPR
                     QANNVCVSGQLLLLDLIEKLEGHCKLIQSNTDGLIVKLNTIDDYELIDDICYEWEQRT
                     GMGLGFDVYTKIFQKDVNNYLVITEDGEVEAKGLYIKDLGNLDYDLPIINKALKNYMV
                     NNTPIEETINNCNDLIEFQKIVKISSKYSHGLYSPTIEGKTKKVFTGGKILNDKCFRV
                     FASKNKNDGGIYKVKNKEKNPEKFANTPDKCFIENGSVIGKKVPRKLDKTWYIDLANE
                     RLKQFGVLLSN"
     gene            9789..11672
                     /locus_tag="KPK67_002404"
     CDS             9789..11672
                     /locus_tag="KPK67_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HBG2459360.1"
                     /translation="MSFDLFKGYIITNNKKAAEKFKNVKKLKTYEQIKNLPEFAGVLA
                     EETVLVDIDDFESSEILYKIVQDLKLKCRVYKTTRGKHFLFKNTSLEKNRTKCRLAIG
                     LNSDIKLGCKNSYSILKFNNIERKILYDSKEIQEIPMYLTPIKNGIDFLSLGEGDGRN
                     QALYNYILTLQSNDFTVEEIRETIRVINKYVLKTPLQDNELEVILRDESFQKKIFFNS
                     KGSFLFDEFAKYIKNNNHVIKINDQLHLYKDGIYVDGQARIEAEMINNISNLNKAKRS
                     EVLSYLNLLISENTSMSEANLIAFKNGIYNIVDDSFIEFSTEFIITNKVNWNYNPGAY
                     SKLVDKTMNKLSCGDFEIRMLLEEVVGYCFYRRNELRKAFILTGDKANGKSTYLDMIK
                     TLLGDENTSALDLKELSDRFKTAELFGKLANIGDDIGDEFIANPAIFKKLVSGDRVNV
                     ERKGQNPFDFNNYSKFLFSANNIPRIKDKTGAVLDRLIIIPFNASFSVKDKDFDPYIK
                     YKLREQEAIEYLINLGLEGLKRVLKNRKFTVPDKVKKEILEYEEVNNPILGFFKEVDK
                     IENESTKEIYRKYQEYCILNGLQPISNIEFSRQVVKKFGYEVKDKRIQGKKYKVFTRA
                     VPG"
     gene            12039..12446
                     /locus_tag="KPK67_002405"
     CDS             12039..12446
                     /locus_tag="KPK67_002405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459361.1"
                     /translation="MTDYEKLEELLNSYSNLDIEIREIELKVRGSSLRGIELNGMPKG
                     NNVSSPIENELIYVERLENEKIYLQIKKESINNMLNLLDDFEKNLIELRYFKKLQYKQ
                     ISYELNISELYVGKKRKKALDKILPFAKKYNLI"
     gene            12969..13205
                     /locus_tag="KPK67_002406"
     CDS             12969..13205
                     /locus_tag="KPK67_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459362.1"
                     /translation="MPNFGTTFVTEEAVYNMSFRSNKAEAKLFTKWVTKALKQIRIHG
                     YYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVY"
BASE COUNT         5773 a         1274 c         2377 g         4215 t
ORIGIN      
        1 taattcgttt tgagtcatac ctctttcttt tcttagtttt tttagcatat ataaaatctc
       61 cttttattaa attttatata agataatatt tgtagaatta taaaatatat gatgttatta
      121 gcgtcattat tttatggaaa tttatggtag aatatagtta agaatattaa gaaaatttat
      181 tttgaaggtc tataaatacc gaccaaataa tttgtaaatt atatgtaaaa ataatgcaaa
      241 aaattgtaag aaaaaaatca aaaataaaat gatttatttt tagaaaatca tgtttagtca
      301 tttttagcgt tatataacat taatgtttat taacgagtat taaatagtac tagtttattt
      361 aaaatcaaaa ctaaaatgaa gaggtgtaga gtttttttgt gggttgcagg aatttttgga
      421 agtctatata atatatttac aaagtaattc ttagaaattt taaatagagt atttactgga
      481 ataaatttac cagagttata aattaagggg attttcatac ctatttctac gaacataagt
      541 tcttaaaatc ttaatatttc ttaattttag ataaaaatag gagatataaa acgtttaaag
      601 aatacaagag aataaagaat aaatatataa ggagatgctt taagtgaaaa tataaataat
      661 atactaaatt tatacaaata aggaaataat atatagtgta tttaaaaata aaaaggctgg
      721 tggtgttaat ttgaagtatt tttaagtgta tatttataat ataaggggaa tttttatatt
      781 tatgaatgga ggaatatagt aatggatgaa gaaagaaaaa aattaattga tgatgcagaa
      841 aagctagcaa acataggaat aaatcaaatt ttaaagttta gagattcatt ggatatagaa
      901 aatataaaat acataaatat agttgcaaca aatttaaagc caaacataaa aaaatagcca
      961 cccttttggg tggttttatt gtgtcatatt tttcattaag tttataagaa attctttttg
     1021 ctcatcattt agattactaa aagtaaataa tatatttttc ataaatttat catcttcatt
     1081 tataatatct gttgctattt ttagtacctt ttcattttca gaaaactcta taaacatttc
     1141 accttcgcca gttaagagcc attctttatt aacattatat agctcacata agtgttgtat
     1201 aaatacaggc ttaggtgtaa ccctgttata ttcaatatta cttatgacat ctctgcttac
     1261 accaagtttt tcaccgaatt cacgttgaga catttttaag gatgttctta ttttatatat
     1321 acgttcatgc atgattttcc tcctaataat tacgttatta attagatagt ataacacaaa
     1381 aaagaattag tcaacataaa tgttgtggta aaaacacaaa aatatttaaa aacaagtgtt
     1441 gactacacaa aaataagatg atatactgtg tttataacac aaggaggtgc taaaatgaaa
     1501 acacaaaaaa ctacttctca tttaaataat ttaaatttag atgatgatga tagaaagctt
     1561 attcaaatga ctcttttgtt taaaaaatta aatttagaac aacaaaatta tatctctgca
     1621 atggctgatg gaatgaaagt gtcaaatgag cttttagaaa aatcaactat gaataactga
     1681 tacaaaggta caaagctgga ataagatttc gaccacaagt gggcgaaaga tgactggagt
     1741 agatttatta ggtgtcattc aaaccgacac caaaataaaa agctagggag gaagaaatta
     1801 tgagtaataa tctacaaata tttaaaaata atgattttgg agaaataaga acagttgaaa
     1861 ttgatggtaa accatatttt gtagccacag atattgctag atgtttaggt tataaagata
     1921 caactaatgc aattaaacaa cattgtaagt gggtggtgaa acaccacata ccacatccac
     1981 aaagtaaaac taagaccttg gaagtaaatg taataccaga aggtgatatg tatagattaa
     2041 ttacaaatag cgaattacca agtgctgaaa aatttgagca ttgggtattt gatgaagttc
     2101 taccatcaat aagaaaaaca ggtacataca gcactaaatc taaagatgaa tcagaaataa
     2161 aatacatgaa tgctcaagca agattaaaaa atgcaagagt aagagaagca aaaatatatc
     2221 tggaattagc tgacaaagta tcacaagttg gtataccaga atttgctcaa gtaatgtatt
     2281 caaagactac tgaattactt tctggagaaa cattaatacc actacctaag atagataaaa
     2341 aaacttattc agctacagag gtaggtaaga tgttaggaat ttcagctaat aaagttggag
     2401 gattagcaaa cgcttacaat ctaaaaacag aagaatatgg tattaaagtg tgggataaag
     2461 ctaaatattc agataaacaa gtacctaatt ttagatatta tgagaatgta attccagttc
     2521 tagaaaaagc actaaaaagt tttgaaaatt aaattcagtt gtgactaata gtcatccaaa
     2581 aagcgaatcc tttaaaagga ttaggttgta taaatataaa aaatttaagg aggaataaga
     2641 aaatgaaaga aaataataga atagtgagtg ctataaatga ggatatagta ggtgttacag
     2701 ttgatggaga gatagactta acagataagg aattagaaat ggtaaacaat tttaagaaag
     2761 ttatgaatag cattaacaat aaaataggta ttaattgtgg gatttcactt agtgataaag
     2821 atatagaaga taataaaaac atattagata agattgcatc aaaagtagcg tggcattata
     2881 agaaaatgaa ttttgaacac ttagagatgg atgaaaaact ctttgatgat tttacttttg
     2941 agtttatgtt aaagttcttt ggtgatatgg aagaatatga attaataaaa tatcttactt
     3001 atataggaga agaaagtaac gaattgagtg atagagaaag gatattttat aaaatagtag
     3061 atactcttga tacaatagct gacattaaaa tatcagacca aaataagata gaaaaagaag
     3121 ttggtatgtg tataggtgaa gataattata taacttattg tatagatgaa gacttaataa
     3181 aattttacat taaagatgaa gaagaactag ttataaataa aaatagccct ctgctatata
     3241 tgttagacac actattttat gaagtacatg aagagtaaaa attgcaataa ttaaataagt
     3301 ataagacaag tttatgagct catattttat aacaaggggt gatgttatga aaatactaca
     3361 acaatttaaa ccatacaaat atgatgaaga gaaaaacata gaatatgatg aaaaaattat
     3421 agctaatcaa aacacagaag tttatatagt atatccacgt atatctgatg aagagaatag
     3481 gagaagatgg aaagagtttg atgaagtagc caaagaagta gctattaata tagcaatgaa
     3541 gaaagctgag acagtagaaa gttaagaaag tagaaagtta agaaagtaga gaagttgcta
     3601 atacttagca ctttctcaaa gaagaaaatt ggacaactat ttttaaacaa ctaggaataa
     3661 ataagcgata gtatgtttga aagaaggtga tgaaattgct tagtcttgat attagtaaga
     3721 aaaatgtgat gactcttaaa aaagatggta agtttttagc agatatagta tttaaggaca
     3781 ttaaaactgg taaaaaaata tcagttggaa cattaaataa aaaaagtgct ggtcaaagta
     3841 accaacacaa taaataaaaa aaacatatga aaattataac ataaaaagga gagatttaaa
     3901 atggttgaag ttaatataaa aattaaaggc gaagcaagtg aaataattga ctcaattaga
     3961 gctatattta atagctttgg tgaagattca atgacagaga taaaagcaga gcaagaatat
     4021 aaaagtagac gtagtagaaa gttaaaaaca gaagatttat cagaccagga ataaagaagg
     4081 tgatataagt tgcaactata taagcatcaa gaggaagctt taaagctaac agaatataat
     4141 aataaatgtg catactacct tgacatgggg cttggtaaga catttgtagg aagtgagaaa
     4201 ttaataaatc taggaagtaa tacaaattta ttaatctgtc agaaatcaaa aattgatgat
     4261 tgggtacatc actttaaaac atattatcca gatatgggga taatcaatgg tacaaagaaa
     4321 cttagtgaag gcatagagta ttttaagatt gttgaagttc catttatagt agtaattaat
     4381 tatgaattag cttttagaag aaaagagtta ttagatttaa aagattttac tttaatgtta
     4441 gatgaatcat caatgattca aaatgaaaaa gctaaaagaa gtaagtttgt actaagttta
     4501 aaccctaaaa atgtgatact actttctggt actccatgta gtggtaaata tgagcaacta
     4561 tattctcaaa ttaagctact agggtggaat atatcaaaag atttatattg gaagcaatat
     4621 attgatgttg agtacaaaga tattggtggt tttccaatga aggtcgtaac aggttataag
     4681 aatgttgaca gattaaaaaa gaagttaaga gattatggag cagtatttat gatgtcagat
     4741 gaagtatttg acttaccaaa acagatagat aatgtaatga aaattagtac tacaagagag
     4801 tataggaagt ttaaaaaaga ttcaattata agtatttcag atgatattga acttgtggga
     4861 gatactacac taactaaaat gttatatgaa agacaattat gtagtcaata caataaattt
     4921 aaattagaag cttttggaga cttaataaac tcaacaagtg acagattaat agtattttac
     4981 aattttaacg aagagttaga cagattaata gaattatcaa gagatagacc aatatcaata
     5041 gtaaatggtt ctataaaaga tttaagcaat tatgaggaat ataataactc aattacattt
     5101 gtacaatatc aagcaggagc tatgggttta aatcttcaaa agtgtaataa gattgtatat
     5161 tttagtctta ctttatcttg tgaattgttt atgcaaagta aaaagagagt tcatagaata
     5221 ggtcaagaaa acacatgttt ttattattat atgatttgta ggaatagtgt tgaagaaaat
     5281 atatataaaa gcttacaaag tggtgttgac tatacaaata atttgtttga gagaggagag
     5341 taatccttgg gtgcagaaaa acaatttgaa aataaagtta aaaagttttt aaaagaacaa
     5401 ggttgttggt ttattaaata ttggggaggt tcagcatata caaaaagtgg tataccagat
     5461 ttattagttt gttgtagtgg taaatttata ggtattgaag tcaaggggga aaaaggaaaa
     5521 gcaagcgaac tacaaaagta taatattaaa gaaattgaaa aggcaggtgg aataggaatt
     5581 attctatacc ctaaagactt tgatagattt aaaaagatga ttttagaaat aaagaaagaa
     5641 ggtgagtaaa tgcagttctc acatagtagg ataggagtat ttaagagttg cccatataag
     5701 tataagctac agtatattga caaggtgaaa acaatattaa atgcagattc taacaatgct
     5761 ttaatattag gaacatcact tcatactggt attgaaaaag atattgatgc agcagtaaaa
     5821 gaatattatt ttagttatcc aagtataaca gatttacaca ttaatgaagt gatgaagtta
     5881 gattatttaa taccaaaagt aaaagaaatt ttaccaaaag ggtttaatga ggttcaaata
     5941 acaactagtg attttatagg gtttatagat ttgttagcac cacatgtata catgactgat
     6001 acaaacttaa atacaaatga aacaaccata tattatgaag atgaagaaag agtatttgat
     6061 atttatgact ttaaatattc aaacaacata gatagatatc tagaaagtga gcaacttcat
     6121 ctatataagt atttttatga aaagcaacat acaaatcatg agataaaaga tttgtactat
     6181 gtatttgtac ctaaaattca gattagacag aagaaaacag aaaacttaca tcaatttaga
     6241 caaagattac ttaatgagct tgataaatct gaaataaaaa tagtgaaggt tgagtatgac
     6301 ccaaataaag ttattaattt ccttgtagat ataaaaagat gtcttgaaaa caaggaattt
     6361 gaaaaaaatc aaaataaatt atgtaactgg tgtgactacc aagaatactg tgaaaagggg
     6421 attgattata tgttattacc aaatagtgaa agaagaagtg tggaaggaat taataaaaaa
     6481 actttatgga tttatggagc accatttagt ggcaaaacca cattagcaaa tgattttgaa
     6541 aagcctttaa tgcttaatac agatggaaat ataaagtttg ttgatgctcc atacatttct
     6601 ataaaagata ttgtaacagt tgaagggaga atgacaaaga gacaacttgc atgggatgta
     6661 tttaaagaag ctataagaga attagagaaa aaagaaaatg attttgaaac aataattgtt
     6721 gacttgttag aagatacata cgaagcttgt cgcctttaca tgtatgacaa gatgggaata
     6781 actcatgagt cagatgatag ttttagagca tgggataagg ttagaacgga gtttttaagc
     6841 actataaaac gacttatgaa ccttgactat aaaaacataa tattaatatc acatgaggat
     6901 acatcaaaag atattaccaa aaaaggtggt gacaagataa cagcaatcaa acctaattta
     6961 caagaaaaag cagcaaataa aatagcaggt atggttgata tagttgcaag agtagtagct
     7021 gatggagacg ttagaacatt atcatttaag actaatgaag tgatatttgg agggggtaga
     7081 ttaaaagtaa aatctaatga aattccactt gattataatg aattaatgaa agtatatgat
     7141 gatattagta tagagaaaaa agacgttaag aagtcaagta agactaaaaa atctaatgct
     7201 gaggaagaag tagaaatgac tcctacagta gaagaaacaa gtgagaaaac tgaaaaagaa
     7261 caaacagagg aagaagtaat tgaacaagaa gaagcaatta aaccagtcaa gaaaactaga
     7321 aaaaagagaa gttagtttaa gaaagattat taattaaaaa tttaaaagaa aaggatggta
     7381 tgtgatatgg attttagtaa atttgataaa gcaatagatg taaaaggatt gaaagaagat
     7441 ataaaagaag catctgaaaa tagtggtaaa tttaaggatg taccacatgg cacttatgaa
     7501 gtagaaataa ataaaatgga gttaagcgag tctaaaaaag gtgacccaat gtttgtatgt
     7561 tggtttaaaa tactagaagg agattataaa gattcattaa tattcatgaa tcaagtagtt
     7621 aaacaaggtt ttcaaattca tatagtaaat gaatttttaa gaagtttaga aactgatatt
     7681 gaagttgaat ttgaatcata cagccaatat gcacaactca taatggatat agctgaggaa
     7741 atagatggag agcttgaatt tgctattgaa tatggtgaga agaaaggttt taataccttt
     7801 acaataaaag acatatttga agtagcttag aaattttatt acagctaggt gtatacatgc
     7861 atctagctgt aatattaaaa aggtaggtaa ttaatatgag agtaaaaaga aaagatggaa
     7921 cttataaacc caggtaaaat tgtaagatgt gtttggtgtg gaaaaagatt ttacaagtta
     7981 gataactcta aagtcatata ttgtagtaga aaatgtgcag caaaagcgag aggggtgttc
     8041 taaatgattt tttatgattt tgaagtattc tcttatgact ggttagttgt atttatagat
     8101 gttctaaata aaaaagaaga agttattgta aatgatatag ataagttaaa ttcattttat
     8161 atagagcata gagaagatat ttttattggt tacaacagta gacattatga ccaatatatt
     8221 tttaaaggac tattatgtgg atttaatgca aaagaaataa atgattatat tattgttaaa
     8281 ggtcaacctg gttggaagtt ttcaaactta ttaagaaaca tacaaattaa taactatgat
     8341 gttatgacta gttttcatgg attaaaacaa ttagaaggtt ttcaaggtca tagtataaaa
     8401 gaatctaatg tatcatttaa cattgataga ccattaacta atgatgaaat agaagaaact
     8461 ataaaatact gtagatatga tgttgaacaa actatagatg tttttattga aagaaaatcc
     8521 gaatttgaag cacatatggg attaattaaa gcttttaaac ttccggtagc atatattggt
     8581 aaaactcaaa cacaactaac agcaattata ttagaagcta caaagaaaga acatgatgat
     8641 gaatttgact tacaaatacc agatactcta aaaattgaga aatataagga agttttaagc
     8701 tggtataaga acccactaaa ccatgactat tctaaaaatc taaaaattaa tatttctaat
     8761 gtacctcatg tattcgcttg gggaggtgtt catggagcaa taacaaaata ctatggtgaa
     8821 ggttattttt tacatgtgga tgttaattcc ttctatccaa gtcttatgat tagatacaat
     8881 taccactcaa gaaatataaa aaatcctcaa aagtatgttg aaatttatga caaaaactta
     8941 cagttaaaaa aagaaaaatc atctctaaga ccagcctaca aattggcagt taataaaact
     9001 tatggaggaa tgaaagataa aaacaataat ttatatgacc caagacaggc taataatgta
     9061 tgtgttagtg gacagctgtt attacttgat ttaatagaga agttagaagg tcattgtaaa
     9121 cttattcaga gcaatacaga tggattaatt gtaaagttaa atacaataga tgattatgag
     9181 ttaatagatg atatatgtta tgagtgggaa caaaggacag gcatgggttt aggttttgat
     9241 gtatacacta aaatatttca aaaggatgtt aacaattatt tagttataac agaagatgga
     9301 gaagttgaag caaaaggatt atatatcaaa gatttaggaa atctagacta tgatttacca
     9361 attataaata aagcacttaa aaattatatg gtaaacaata cacctattga agaaactata
     9421 aataactgta atgatttaat agagtttcaa aagatagtaa aaataagtag taaatattca
     9481 catggtttat atagtccaac tatagaagga aaaacaaaaa aagtatttac tggtggtaag
     9541 attttaaatg ataaatgttt tagagtattt gcttcaaaaa ataaaaatga tggtggtatt
     9601 tataaagtca aaaacaaaga aaaaaatcca gaaaaatttg ctaatacacc agataaatgt
     9661 tttatagaaa atggaagtgt tataggtaaa aaagtaccaa ggaagctaga taaaacttgg
     9721 tatatagatt tagcaaatga aagactcaag cagtttggag tattattaag caattgaggt
     9781 ggtgattagt gagttttgac ctatttaaag gatatataat tacaaataat aaaaaggcag
     9841 ctgagaaatt taaaaatgta aaaaagttaa aaacttatga acagattaag aatttaccag
     9901 agtttgcagg cgttttagca gaagaaactg tgttagttga tattgatgat tttgaaagta
     9961 gtgaaattct atacaaaata gtgcaagatt taaagctaaa atgtagagtt tacaagacaa
    10021 cacgaggtaa gcattttcta tttaaaaata ctagtttaga aaagaataga acaaaatgta
    10081 gactagctat aggtttaaat tcagatataa aattaggatg taaaaattcc tattcaattt
    10141 tgaagttcaa caatattgaa agaaaaattt tatatgatag taaagaaata caagagatac
    10201 caatgtattt aactcctata aaaaatggaa ttgatttttt aagtcttgga gaaggtgatg
    10261 gaagaaatca agcattatat aactatattt tgactctaca gagtaatgat tttactgttg
    10321 aggaaataag agaaacaata agagttatta ataaatatgt tttaaaaact ccattacaag
    10381 ataatgaact tgaagtaatt ttgagagacg aatcctttca aaagaagata ttttttaatt
    10441 caaaaggttc atttttattt gatgagtttg ctaaatacat aaaaaataat aatcatgtaa
    10501 tcaagataaa tgaccagtta catttatata aggatggtat ttatgtagat ggtcaagcaa
    10561 gaatagaagc agaaatgatt aataatataa gcaatttaaa taaggctaag aggagtgaag
    10621 tacttagtta tttaaatttg ttaataagtg agaatacaag tatgtcagaa gctaatttaa
    10681 tcgcttttaa gaatggtata tataatatag ttgatgattc ttttatagaa ttttcaacag
    10741 aatttattat aacaaataaa gttaactgga actataatcc aggagcatat tcaaagttag
    10801 ttgataaaac tatgaataaa ttatcatgtg gagattttga aattagaatg ttacttgaag
    10861 aagtagtggg atactgtttt tatagacgta atgagcttag aaaagctttt atattaactg
    10921 gagataaagc aaatggtaaa tctacttatc ttgatatgat aaagacatta ttaggggatg
    10981 aaaatacatc agcacttgat ttaaaagaat tgagtgatag atttaaaact gctgaattat
    11041 ttggaaaact tgcaaatata ggagatgata taggagatga atttattgct aatcctgcta
    11101 tatttaaaaa gctagtaagt ggtgataggg taaatgttga aagaaaaggt cagaatccat
    11161 ttgattttaa caactatagt aagtttttat tttcagctaa caacatacct agaataaaag
    11221 acaagactgg agcagtactt gatagattaa ttattattcc ttttaatgcc agtttctcag
    11281 taaaggataa ggattttgac ccttatataa agtacaaatt aagagagcaa gaagctatag
    11341 agtatttaat aaatctagga ttagaaggct taaaaagagt attgaagaat agaaaattta
    11401 cagtaccaga taaggttaaa aaagaaatac ttgaatatga agaagtcaat aatcctattt
    11461 taggattttt taaagaagtt gataagatag aaaatgaatc aacaaaagaa atatatagga
    11521 aatatcaaga gtattgtata ttaaatggat tacagcctat atcaaacatt gagttttcaa
    11581 gacaagtagt caaaaagttt ggatatgaag taaaagataa aaggatacaa ggtaagaaat
    11641 ataaagtatt tactagagca gtcccgggat agtcccagat gtagtcccta gatgtaatat
    11701 agtaatttca acgttgtccc ggatgtccct agatgttttt gaccttttat atattcaact
    11761 aaaaaatcat caattttttg tgatgatttt gctcccttta tataaagaat atatatataa
    11821 tagtatggga ctgaacaaaa attattgtct ggatatattg aaatagctag gcttgaagca
    11881 gtcccggatg tattttacat ctgggactat ctgggactgg gtttaattaa gaaattatat
    11941 aatgtaaaaa tatttaaaaa ataagatata gttcattaat aacttaataa aataattaaa
    12001 aagaaaattc tgttaattta caaggatgtg aaaatggagt gacagactat gaaaaattag
    12061 aagaattgtt aaactcatat agtaatttgg atattgaaat tagagaaata gagttaaaag
    12121 taagagggag tagtttaaga ggaatagaat taaatggaat gcctaaagga aataatgttt
    12181 cttcaccaat agaaaatgag ttaatatatg ttgaaagatt ggagaatgag aaaatatatc
    12241 tacaaattaa aaaagaatcg attaataata tgttaaattt attagatgat tttgagaaaa
    12301 atctaataga actaaggtat tttaaaaaat tacaatataa acaaataagt tacgaattaa
    12361 atataagcga attatatgta ggaaaaaaga gaaaaaaagc actagataaa atactccctt
    12421 ttgcaaaaaa gtataatcta atatgaaagt cttgtttgtg tccgatttat gaccgattaa
    12481 tgattgattt agtatagaaa catgtcacat caatatacta tgagataagt tataatggta
    12541 gtatgaaaca agtgtataat atattccccc ttgaaaaagg ctaagttacc ccaacttagt
    12601 cttttttatt ttgtaaataa ggagaatgat aaatgagtaa ttcatgactt agagtgattt
    12661 ctaggtcttt ttttatacaa ttttaatccc tcatattgaa gggtcgaaaa taagggtgtt
    12721 caaattgaat attctttatt aataactaat ttaggaggta gtagtatgtt gaaaatttta
    12781 caagagaaaa atgtaaggat gatatggtca aaaaatggag aagaagtttg atttaatgca
    12841 aatgacgtag gagaggaact aggcatagta aatattcgtg atacattaag aaatatagat
    12901 agagaatata aaaagaaatt aatgagtcta ctgtcggaga ttcctacact agaaacttta
    12961 aagataaatt gcctaacttt ggtactactt ttgttacaga agaagctgtg tacaatatgt
    13021 catttagaag taataaagca gaagcaaaac tatttacgaa gtgggttaca aaagcactta
    13081 aacaaattag aatacatggt tactatattg ccacagaaaa agatcaagaa tggctggata
    13141 taagaacaga aggtaaaaag gtcagaaaag attttacaga tgaaatacaa gagtttgttt
    13201 attaatacaa gcacttgaaa gagttatatc tatgaaatca cctaggttga tagataaagg
    13261 tatgaattat aaagaggtat ataaaagatt aaagaattaa tagaaatgat ttaaatgact
    13321 gtattaatag agggtctttt ttttatgtaa tgaattaaaa atgaaatata tttgtacatt
    13381 atagacagga ataaggtaaa agtattattt agacttattt tgtggttgtc gaatgattat
    13441 tgaaggatat tgactgatta tatagaatta tatcattata aatatttgtg aaaggaataa
    13501 tggtataatg agtaatttta aaaaaagata tattgatgtt tatagaatag atattataaa
    13561 taaaattaat gggagtgtct attcagatga agaaatttta aaagaggtaa taaagacctt
    13621 ctttaaggat aaagaatat
//
LOCUS       DAEMHW010000102        13267 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEMHW010000102 DAEMHW010000000
VERSION     DAEMHW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.102"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            191..1564
                     /locus_tag="KPK67_002407"
     CDS             191..1564
                     /locus_tag="KPK67_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2459363.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILANSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1820..2887
                     /locus_tag="KPK67_002408"
     CDS             1820..2887
                     /locus_tag="KPK67_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBG2459364.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTIDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            3031..3339
                     /locus_tag="KPK67_002409"
     CDS             3031..3339
                     /locus_tag="KPK67_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBG2459365.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3332..4762
                     /locus_tag="KPK67_002410"
     CDS             3332..4762
                     /locus_tag="KPK67_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBG2459366.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4840..5214
                     /locus_tag="KPK67_002411"
     CDS             4840..5214
                     /locus_tag="KPK67_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBG2459367.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWNLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5208..7412
                     /locus_tag="KPK67_002412"
     CDS             5208..7412
                     /locus_tag="KPK67_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG2459368.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7412..8821
                     /locus_tag="KPK67_002413"
     CDS             7412..8821
                     /locus_tag="KPK67_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBG2459369.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKDIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8806..9870
                     /locus_tag="KPK67_002414"
     CDS             8806..9870
                     /locus_tag="KPK67_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBG2459370.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9900..11321
                     /gene="nhaC"
                     /locus_tag="KPK67_002415"
     CDS             9900..11321
                     /gene="nhaC"
                     /locus_tag="KPK67_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG2459371.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11750..12214
                     /locus_tag="KPK67_002416"
     CDS             11750..12214
                     /locus_tag="KPK67_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG2459372.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYTDGKNEILQKIVPAQKIITAIDL
                     GPYELQDPTLEDLFLWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12513..12875
                     /locus_tag="KPK67_002417"
     CDS             12513..12875
                     /locus_tag="KPK67_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2459373.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
BASE COUNT         5250 a         1442 c         2547 g         4028 t
ORIGIN      
        1 ataaccataa tgaaagatat ataaataaaa ttttaaatat acttaaaaat atattaatat
       61 aatgtacata aaaaatatca taaatatatg ataagataaa tacaatagca acattttatt
      121 aattttttgg gtttttatat atttaggtac tggggtgata tagttgaacc aagagatatt
      181 ttttaaaata atgaatgaaa tattggaaaa tattgatgaa ggggtacatt ttgttaataa
      241 agacgggaaa actataatct acaataattc tgtacaaaaa atggaaaaaa tgaacagaca
      301 cgatatttta gccaattcat tttttgatat agtcaacaaa atgaaaataa acaggagtac
      361 actattagaa gttttggaaa aaagggaagt tatcaaagac aatattcaaa aatatctgga
      421 taaaaatgga aaagagataa cagcaattaa tactacaata ccaattgata ttaaaggcga
      481 gtttgtaggg gcacttgaaa tatcaaagaa tatgacaacg atagaaaatt tactaaacag
      541 aaattataaa aaaaatttag tcaataaagt tagtagtaga caaaaaaagg gttatgaatt
      601 tgatgatatt ttaggggaaa gtaatttgat taaagaagca atagctaaat ctaagaaagc
      661 ttgcaagtct gatgcttcag tatttatata cggtgaaact ggaacgggta aagagttatt
      721 tagccagtcc attcactatg gaagtaatag gagaaactat ccatttatag cacaaaattg
      781 tgcagctttg ccggaatcac tattcgaagg tatccttttt ggaactagca agggaggatt
      841 tacaggagca atagataggc caggtttatt tgaacaagct aacaaaggga cattgttatt
      901 ggatgagata aattccatgc ctattatgtt acaggctaaa ctattaaggg ttttacaaga
      961 aggttatgta agaagggttg gaggaactaa ggatatacct atagatgtaa gaattattgc
     1021 aacgacaaat gaaagtccaa aggtaatttt aaacgagcat aaaatgcgaa aagatttata
     1081 ttataggctt aacataattc atataaaaat tcctcctctt agagatagaa aagaggacat
     1141 acctctattg tgcaaaaaat ttatttcaaa gtataataaa aagttaaata aaaaagtggt
     1201 aggaatttca gatgaagcta ttaaaatttt aaaaaattac gattggcaag gtaacataag
     1261 agagctagaa aacataattt attgtactat gagtatgatg gaagatgagg tacaaataga
     1321 aacaagtatg attaatctaa acgactatta caatcttaaa gagaataaag agagtaacaa
     1381 ttattcttta aacgatttgg aggacaaaac acttagtaag attattagtg agatagaaga
     1441 aaagtatata tatgaatcat tagaaaaggc atcgtacaat ataactaaag ctgcatcaat
     1501 tttaggtatg aataggcaaa atttacagta taaaataaaa aaatataata tcaaaatatt
     1561 ataaataagc aagataaaaa aacaaaatca aagtaaaata aaaaagcaaa atagaggagc
     1621 aaaattatta taaaagagca aaatattttg ctcttttttt tatttctcat aagaaatatt
     1681 attttttatt tatgaaaata cataataaaa taattatagt ttcaacattc caataaatat
     1741 ttactttaat tttaaagttg gcacgattta tgctttaact aattattgag aatatataaa
     1801 tttttaggag ggtgtatata tgagaaaagt aagagtagga atatggggat ttggagctat
     1861 gggaataggt atggctaata tgattctaaa gaaagaaggt atagaaatag tatctgtttg
     1921 tagtagaagt acttcaggga aaagtatgta tgatgtttta ggaattgaaa gaggagaaag
     1981 accagaagtt attatcaata aaaattatga agaagttttt agagaaaaaa gtgtagatgt
     2041 tgttttgtta gcaactgatt catttactaa aaaggctttt gataaaataa ttttcttatt
     2101 aaatagaaaa ataaatgtta tatcaacagc agaacaaatg gcatatccac aagctgatga
     2161 tgcagattta gctaaaaaaa tggatgaagt tgcaaaggaa aatggagtaa gtatacttgg
     2221 aacaggaata aatccaggat ttgtattaga tttactagta ttggcgcttt caggtacttg
     2281 tgaagaagtt actagtataa aagctaagag agtaaatgat ttatctccat ttggaaagtc
     2341 tgttatggag gaacaaggtg ttggagtaac aagagaagaa tttataaaag gtgtagaaga
     2401 taagactata gcaggtcatg taggttttgt agaatcaata aatatgatag ctgatggatt
     2461 aggttggaaa ttagataaga tagaacaaac aaaagagcca ataatgacta caatagatag
     2521 aaagtctaag tacggagagg cattggctgg aaatgttgct ggttgtagac aatgtggata
     2581 tggatatgta aatggagaag ttttaattga aatggagcat ccacaacaga taataccaga
     2641 agcagaaggt attaaaacag gagactatgt atcaataaaa ggtataccaa acattgattt
     2701 acaaataaat cctgaaatac ctggaggagt aggtacttat gcaatgatag taaactctat
     2761 tccattaata ataaatgcta gaccaggtct taaaactatg ttggatatac cagtacctag
     2821 agcaataatg ggagatataa gaaatcagat agaagttgaa ttggaagaag aatcaaaggc
     2881 taattagtaa gataacagat aactaattag taagatagca actagctaat tagtaagata
     2941 acagttagct aattggtaaa ataatgttta atttaaaata acagtaaatt aattagtaag
     3001 gcaatagtca attaataagg ggatgaaaaa atggatgcta aaattaatga ttgggttatt
     3061 atacacaata tagtattgac tccagaagaa agagctccac aagttccaga ggatactaag
     3121 aaagtatcct tagaaatgtg ggttaaaggt tttatacaaa gtgatgcttc tataggcgac
     3181 ttagtagaag ttaaaacaat tacaggcaga cttgtaaaag gtaatttact taaaataaat
     3241 ccatattata ctcatgatta tggtaaatgt ataccagagt tactccaaat aggaatccaa
     3301 gctaaagaaa tattatttgg aggtgtatat aatgaataat tcaacatcaa tgaaagatat
     3361 gagttatcaa gctgttatgg gaagaaataa cgaaataatg aaaaatgcaa tcggattgga
     3421 ttattcttct tttgaacaag agggaatagg gtttgattat gagaaaatga tgagtgaaac
     3481 aggatataca ttacaagata ttgaagcaat tcaatcacag tatgctgttg gaaatactcc
     3541 tttaatagag cttaaaaatt tgactaaatt agctagaaaa tgtgctaaag aaggtaaagg
     3601 agctagaata tttgtaaaag atgaagcaat gaatgcatct ggtagtttta aagcaagaag
     3661 agcagctact gcagtatatc atgccaaaca aatgggatat aaaggtgtaa tagcagctac
     3721 tagtggtaac tatggtgcag ctgtagcatc acaagcggca atgcagggat taaaatgtat
     3781 aatagttcaa gaatgttatg attctaatgg tgtaggtcaa cctgaaatta tagaaaaagc
     3841 gcgaaaatgt gaggcatatg gagcagaagt tgttcagtta actgttggac cagagttatt
     3901 ttatacgttc ttagttttat tagaagaaac aggctatttt aacgcatcat tatattcacc
     3961 atttggaatt gcaggtgtag aaactttagg ttatgagtta gctatccaat ttagagaaaa
     4021 gtataaaaaa gaccctgata tagttgtttg tacaaatgca ggtggtggta atcttactgg
     4081 aactgcaaga ggattgataa aagctggttc tattaatact aaagtagtag gagcaagtgt
     4141 agatttaaaa ggattacaca tggctagtga taatcaattt aataaaaaat catttacaac
     4201 aggacataca ggttttggga taccatactg tacatggcct gataggtcag atgtacctag
     4261 gtctgcagct agaccactta gatatatgga tagatatgta ttagtaaaac aaggagaagt
     4321 tttttataca actgaattat tggcacagct agaaggtata gaaagaggtc cagcaggaaa
     4381 tacatcttta gcagcagcat tctctttagc tcaagagcta gatgaggata aagctatagt
     4441 ggtacaagaa acagaatata cgggtgcagg taaacatata tgtccacaac ttacctttgc
     4501 aagagaaaat ggaatagata taaaatttgg aaatccaaga gaagaaattg caggagtaaa
     4561 cttaatatta ccagaaagac cagaattatt gaaatgtgtt gatattgata tgaataaaat
     4621 aagaaagtca tttataaaaa attgtatatc taataatcac attgataata ttgataagct
     4681 aagtaatcga gatatagaat ttttaatgga agaagtaaaa agttcaagag attttgttgt
     4741 tgatgtatta aataatttat agtaattatt gtttttttat ttaatataaa ataaaaattc
     4801 ggaaaattcg attaaatcaa tacgaatgga ggaatgttca tgaaaaaaag agaggatgat
     4861 tttgaagtta gaagaaaaca tcttcaagaa ctgagtgatg atgagttaaa agaaaggttt
     4921 tggaatttag ctacccagat agtagaacca atgatagaac ttggtaaaaa gaatacaaca
     4981 ccatcaatag aaaggtctgt cttgttgaga atgggatttt cttctcttga agtaaaacct
     5041 attttagaag gggttatgga aagaggtctt ataggtaaag gagcagggca tgtagtttat
     5101 aaattggcta aatctaaaaa tataacggtt agagaagcag gtttgttatt agttaagggt
     5161 gaatattggg atgaagttac atgtttattt agagaagggg tagaatcatg ttaaaggaaa
     5221 ataaaaaatt ggatatagac tatatattaa aagatttgga taaatataaa ccaaaacgaa
     5281 gaggttgggt ttggagagaa cacttggaaa atcttgaaat gggacctttt aaatataaag
     5341 actgtacaaa acctttaaag aagagtgtag ggctaccatc atctaagtat ttcaataata
     5401 tagaccctca accaagtcca gttattacta ctgaaatagc ttctggtaga tttgaggatg
     5461 atataagaag aatgagaatg gcagctcatc atggagctga ccatctaatg gttatcagaa
     5521 ctgctggaca atcacatttt gatgggctta tagaaggaac cccacaaggt ataggtggag
     5581 ttccaataac aagaaaacaa gtaagagctc aaaggaaggc tcttgatttt atagaagaag
     5641 aagttggtag acctattaat taccattcat atgtaagtgg tgttgctggt cctgaagtag
     5701 cagtaatgtt tgctgaggaa ggagtcaatg gagcacatca agacccacaa tataatgttc
     5761 tgtataggaa tataaacatg gtaaggtctt ttgtagatgc ttgtgaggca aagaaaataa
     5821 tggcttttgc caatatagct caaattgatg gagcacataa tgctaatgct actgctagag
     5881 aagcatggaa ggtaatgcct gaacttatgg ttcaacacgc tttaaactct atattttcag
     5941 aaaaaatagg tatagataaa agtaatatat gcttatcaac agttccacca acagcacctc
     6001 cagcaccatg tttaaaaata gacttacctt acgctgttgc acttagagag ttatttagtg
     6061 attataagat gagagctcaa atgaatacta aatatatgga atcatcaact agagaagcaa
     6121 cagttactca tgtattaaac ttattaacat cagtattaac tagagctgac atacaatcaa
     6181 ctataacacc tgatgaaggt agaaatgtac cttggcatat atacaatata gaagcatgtg
     6241 atacagcaaa gcaagcttta gttggtatgg atgggcttat ggatatgata gaacttaaag
     6301 atgtcggtga attaagagac aaagcaagag aacttaaaga aagagctgta ttatatatgg
     6361 aagaaatatc tgaagttggt ggatattttg aatctgtgga acaaggattt tttgttgatt
     6421 ctggaaatta tccagaaaga aatggtgatg gaatatctag aaaaataaaa ggtggagttg
     6481 gtgaaggtac tgtttatgaa agagaagaag attatttagc acctgtaact gctcactttg
     6541 gatacaataa tgtagctcaa tatgatgaaa atgctataga taatccatca atgttaatag
     6601 atggatgtac atttgaaaat ccagacaaaa taatatatat tgatgagctt gacgattttg
     6661 ataatgtaga aaatagacta aaagaatcat atgagtacag aaatggaact aaaattaagc
     6721 ctgaaatgga atggtgtgca gatggaattg taatgataac aatgatgctt cctactgata
     6781 aaagaacagc agagtttgca gctttggaat ttgtcaaaaa aatgaactta caagaaattg
     6841 aggtcataag tagagaagtt atgcatgaat ctgaagggac tagaatagaa gtgaaaggaa
     6901 gagttccatt tgatatagat ttaaacaatt tagtaatacc agaagaacct aaggtactaa
     6961 ctgatgaaga aataagagca gatatagaag aaaaacctat gaaaatagtt tcagctacgg
     7021 ttggagaaga tgaacattca gttggtttaa gagaaataat agatataaaa catggaggta
     7081 ttgaaaaata tggcatagaa tgccactatc tagggacttc tgtacctgta gaaaaattgg
     7141 tagatgcagc gattgaactg aatgcagatg caatacttgc atctacaata ataagtcatg
     7201 atgatattca ctataaaaac atgaaaaaat tacacgatta ttgtgtagaa aaaggtataa
     7261 gagataaagt aatgattgcg tgtggaggaa cacaagtaac acctgaaata gctgttgaac
     7321 aaggcataga tgctggattt ggaagaaaca gcaaaggcat acatgtagcc actttcttag
     7381 ttgaaaaaag aagagaaatg agtaataaat aatggaaaat agcaaagttt ctttaaaaat
     7441 agatgtgttg gtagctgaaa ttggaagtac tactacagtg gtaaacgctt tccacgatat
     7501 taatactaac aatcctatat ttttgggtca aggtcaagct ccaacaacag tgtttgaagg
     7561 tggcgatgta agaaatggct tatcaggtgc tattaaagat ttagctaata aattatctgt
     7621 agatgatata gagtataatg atatgtttgc aacttctagt gcagctggtg gattaaaaat
     7681 gactgttcat ggattagtct atgatatgac agtaaaagca gctaaagaag cagctttagg
     7741 ggctggagct gttattcgtc aaataacatc aggtagaatt aaaagaactg atttaaataa
     7801 aataaaagaa ataaacccaa acataatttt aatagctggt ggagtcgatt atggagaaag
     7861 agatacagct atttataatg cagaaatgat agcttctatg aatctaggca taccagttat
     7921 atatgctggt aatgttgaaa atcaagagga aataaggtta atatttgaag atactaatta
     7981 taagctttat ataactgaaa atgtataccc taaaatagat ttattgaata tagaacctac
     8041 aagaagaatc attcaaagtg tatttgaaga acacataact acagcaccag gaatgaaata
     8101 cattaaagaa atggttaatg aaaatattac tccaacccca ggagctgtaa tggaagcttc
     8161 taaactttta tataaagata ttggagattt attaactctg gatgtaggtg gagcaactac
     8221 tgatgtacat tctgttacag atggaagtga ctatattaat aaaattttgg ttaatcctga
     8281 acctactgca aagagaagtg ttgaggggga tttaggtgta tatgtaaata tgaagaatat
     8341 agtagaagtt ataggtaaag aaaacttaca aagtgaacta tctatagata ttgatgcagt
     8401 aatagagaat tatcctccaa taccaaaatc aaaagaagaa atattatttg ttgagaggtt
     8461 gacaaaagaa gctgttgtta aagctatgct aagacattca ggaaaaataa gaaatatcta
     8521 tggaacaact ggaaaagtca aaattgcaga agggaaagat ttaacagaag taagatatat
     8581 agttggtaca gggggagcac ttactaggct ccctagtaga attgaaatat tagataatat
     8641 gctcaaatac aataaaaata atgaattact atttccaaag gaaaaaacaa agatattaat
     8701 agataatgac tatattatgg catctatggg tgttttatct aaaaaatatg aagaagcatc
     8761 tcttaagctc ctgttaaaaa gtttgaattt tgaggaggaa agattatgta tcctaggtta
     8821 gaaatagata tagaaaagtt aaagcataat gcaaaattaa tttcgcagat gtgtcatgaa
     8881 agaaatatta agatatcttt tgttactaaa tcattttgtg cacaaaaaga aatagtagaa
     8941 gaaatatgta gtgaaggaat agaccatata gcagattcaa gaatacaaaa cttaaaaaat
     9001 ttacaagata taaatctacc taagatattg attagaatcc caatgttaag tgaattagaa
     9061 gaagttatca attattgcga tattagtttt aactcagaat taggaacaat aaagaaatta
     9121 aatgaattat gtgaaagtaa aaatattatt cataagatag ttcttatgtt tgacttagga
     9181 gatttacgag aaggatactt ttatgaagaa gactttttta ataatgtaag agagatagta
     9241 agattaaaaa atattgaaat aattggtata gctacaaact taacctgtta tggagctatt
     9301 ataccatcta gggaaaatat aggcagactg gtaagtatag caaaaagaat ggaagaaaaa
     9361 tttggaataa atctagaaat agtatctgga ggtaattcaa gttctattca tctgttgata
     9421 aataataata tgccagaagg tattactaat ttgagaatag gcgagtccat tctacttggt
     9481 agagaaacgg catacggtga aaatataaat ggaacatatc aagatgcatt taaattaata
     9541 tgccaagtag ttgaatgtaa agaaaaacca tcagttccta ttggagaaat aggagttgat
     9601 gcctttagaa ataaacctgt ttatgaagat aagggaatct taaaaagggc aatcatagct
     9661 ataggtaaac aagatataaa tattgactct ttaattccaa tagacactga tataaaaata
     9721 ttaggggcaa gttcagacca catgatacta gatgtaagta acacaaaata tgattataaa
     9781 ttaggtgaca atttagaatt tttattaact tatgggggta tcatgtcatc tagtacgagc
     9841 aaatatgttt caaaaaaaat tatagtgtag tgtttaaata attaggagga aactatttta
     9901 tgaaagagcg agaatgtaga aaagcaagtc tgtttgatgc aatgatacca attacttgtt
     9961 tgatattatt tttaagtcta ggagtattag tgtatggttc atcacctcat gtaccactaa
    10021 taggagcagc agcagtagca ggacttgtgg ctgtatatag acttggattt aaatggaaag
    10081 agttagaatt aagtatgttt aatagtataa agatggcaat gcaagcaatc ttaatcataa
    10141 ttataatagg cgtacttata ggaacttgga tagtaagtgg agtagttcca tgtatgattt
    10201 actggggact aaaaatttta tcaccaaata tttttttagt agcatcaaca ttagtctgtg
    10261 caatagtatc tcttgcgaca ggttcatctt ggagcacaat gggtacagta ggagtagctc
    10321 ttttaggaat tggtcaaagt ttaggtatgc caattggatt aatcgtaggt tctataatat
    10381 ctggagctta ctttggagat aaactatcac ctttatctga aacgactaac ttagctccag
    10441 caatggcagg aacagatttg tttacacata taaaatatat gctatattcg actataccat
    10501 cattattaat ttgtttagta atttatggtg taattggaat gaagtattca ggtcaagcac
    10561 tagatataaa acaaatagaa ttaattaggt ctacattaga ttctacattt aatactttat
    10621 caccagtatt attattagct ccagcaatag taattggatt agttgtattt aaagttcccg
    10681 ctataccagg gcttatagta ggtgctgtat tgggagtatt gtttgcagta gtttttcaag
    10741 gagaatctat gaatacgata ttagatgcag cacaaaatgg atatgtttcg tcaacacaaa
    10801 tacaggaggt agatgcttta ttatctaaag gtgggattat gaatatgatg tccactgtat
    10861 ccttaacaat atgcgcttta tctcttggag gtattttaga aaaaacaggt atgcttgaag
    10921 tagtagcatc ttctttacta agattagcaa agggtgtatt tggaacagta ttatgtacta
    10981 tggtaacttg tacaattacc aatataatag caggtgaaca atatctatct atagttatac
    11041 caggaagaat gtataataag gaatataaaa agagaggtat acatcctaag atgttgtcga
    11101 gagctttaga agatagtgga actttaactt ctccattagt accatggaat acatgcgggg
    11161 cgtatataac agcaacatta ggtgtatctg cactaacata tggtccatac gctttattaa
    11221 atattataaa tccaatagta tctttagtat taattgcatg taagtttaaa atagcaagaa
    11281 tagaagatga acctgaaact tgtttaaatt ttgatgtttg ataatagata acttatgtaa
    11341 gaacgtagga actagggggg tagcctgtgg ggaattagta agacatattt tagtgtgcga
    11401 aattctataa atcagtaatc ttgtgacttt agccatggga ggttcagatt agtaaattta
    11461 tagttttata ttagtctata ttttgatctt ttaattgttt gtataaattt tttatagaaa
    11521 aagatttctt ttagacttgc ctaaaaatta ggcaagtctt ttaagtatgt tctgattata
    11581 agagcactgt gtgacaagaa tgatgagatt atttttgcta tactttcgat ataagaaatg
    11641 gaaggaggtt atgttttata ttcatatgaa ataattagtt acacagtaaa ttttcgtata
    11701 atttggtata aaaagattag aaatgaaaat aaagtaatgg aggaataaaa tgactagaat
    11761 atccaatata acaatagctg aacaatctga gtactgtttt ttggcaatac gaaaaacaat
    11821 tgactttatg gtagagtttt cagagttttc aaaacaaagt tttgaaaaaa tttctaaata
    11881 tctggaggag agaggaattt tatcttcagg agcaccgatt gtatgttttc ataacatgga
    11941 tttagcaaaa ttggatgttg aagtagggtt tcctgtagcc acttatacag atgggaaaaa
    12001 tgagattcta caaaagattg ttcctgcaca gaaaataata actgcaattg atttaggacc
    12061 ttatgaatta caagacccta ctttagaaga cttattttta tgggcaaata gcaatggata
    12121 taaacttcat ggcgatatat attaccagta tttaaatgat atcaatcgtc cagctagtga
    12181 atatcttacg aaaatgatgt tacccattat atagaaagtt aaagattatc gtttgttagt
    12241 tgataattag aagaacattt actgctacta cataaatttt atcttaaata atatcaaggt
    12301 gatttaattt atgtattcta atgtaactgt agataatcaa tttataacta acttagtgta
    12361 atttagattt aatttatgta ttctaatgta accccaatat attgttaagg tctacttaag
    12421 atgctcaaaa ggctatgctt aatttattgt tatataattt gtagagtggt ataatttaga
    12481 ggtaatatta tatagatata tagggggaaa atatgaaaat atatgatata ttaacacgtt
    12541 tttatattca agatatagaa aaagccatac ctttttatga gaatctttta aaagaaaaat
    12601 gttctttgcg tttttcctat aaagaagttg ggttggaatt agcgcaaata ggaaatgttt
    12661 tactattatc tggttctgat gatgctctta aaccatttat agaaacaaaa tctactttta
    12721 tggttgattc tgttgatgaa tggagaagtt atctattgga taatggagca gttgtagtaa
    12781 gagataagaa aaaagttcct acaggatata atatgacatt gagacatcca gatggtacaa
    12841 ttatagaata tgttcaacat acaaagcagg attagttaaa aatagatttt actactgagc
    12901 aactaattag aatctattct tagatgtaca atttattatt tttggtatta atagattata
    12961 agtcaaatta taatatttca aaatagaata gaaatttatt tacataaaag agagtgttta
    13021 aaaatgaact ttttagttca tgaagcactc ttttttgtat gaaatcaaat acatcaatat
    13081 ataaaataaa ctgatgaaga gcttaaatta aaataaaaag attcataaca ggaccccatt
    13141 aaactttaag atagggtaag tttgtaatca tattaaataa ttgtaaaaag ctgtttttag
    13201 tcgtataata taaatattta tcatttaatt aaggaatcta ataagaagaa aataataaat
    13261 aactttt
//
LOCUS       DAEMHW010000103        13217 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEMHW010000103 DAEMHW010000000
VERSION     DAEMHW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.103"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            247..702
                     /locus_tag="KPK67_002418"
     CDS             247..702
                     /locus_tag="KPK67_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG2459374.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSSLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            751..1335
                     /locus_tag="KPK67_002419"
     CDS             751..1335
                     /locus_tag="KPK67_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBG2459375.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1340..1663)
                     /locus_tag="KPK67_002420"
     CDS             complement(1340..1663)
                     /locus_tag="KPK67_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2459376.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1815..2333
                     /locus_tag="KPK67_002421"
     CDS             1815..2333
                     /locus_tag="KPK67_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2459377.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2509..3177)
                     /locus_tag="KPK67_002422"
     CDS             complement(2509..3177)
                     /locus_tag="KPK67_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459378.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3292..4707
                     /locus_tag="KPK67_002423"
     CDS             3292..4707
                     /locus_tag="KPK67_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2459379.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDLKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAGFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4930..5388
                     /locus_tag="KPK67_002424"
     CDS             4930..5388
                     /locus_tag="KPK67_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2459380.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCIYYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            5385..5969
                     /gene="clpP"
                     /locus_tag="KPK67_002425"
     CDS             5385..5969
                     /gene="clpP"
                     /locus_tag="KPK67_002425"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG2459381.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6281..7357
                     /locus_tag="KPK67_002426"
     CDS             6281..7357
                     /locus_tag="KPK67_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2459382.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7424..9238
                     /locus_tag="KPK67_002427"
     CDS             7424..9238
                     /locus_tag="KPK67_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore surface glycoprotein BclB"
                     /protein_id="HBG2459383.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGATGADGVTGPTGPTGATGADGITGPTGATGATGFGVTGPTGP
                     TGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGITGPTGATGATGADGATGV
                     TGPTGPTGATGADGVTGPTGATGATGIGITGPTGPTGATGIGITGATGLIGPTGATGT
                     PGATGPTGATGPTGVGVTGATGVTGPTGATGATGANGLVGPTGATGAAGTPGATGPTG
                     ATGPTGVGITGATGATGATGPTGADGATGPTGATGNTGADGVAGPTGATGATGNTGAD
                     GATGPTGATGADGATGPTGATGATGVTGATGPTGATGATGASAIIPFASGIPLSLTTI
                     AGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRDGTITSISAYFST
                     TAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPLTGILSVGSISSGIVTGL
                     NIAATAQTRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            9407..10168
                     /locus_tag="KPK67_002428"
     CDS             9407..10168
                     /locus_tag="KPK67_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG2459384.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNILIS
                     NLNDFLCEELEKIGANSYAIPGLYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            10477..10755
                     /locus_tag="KPK67_002429"
     CDS             10477..10755
                     /locus_tag="KPK67_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459385.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            11048..11623
                     /locus_tag="KPK67_002430"
     CDS             11048..11623
                     /locus_tag="KPK67_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBG2459386.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            12062..12772
                     /locus_tag="KPK67_002431"
     CDS             12062..12772
                     /locus_tag="KPK67_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2459387.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            12766..>13217
                     /locus_tag="KPK67_002432"
     CDS             12766..>13217
                     /locus_tag="KPK67_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2459388.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKY"
BASE COUNT         5130 a         1563 c         2331 g         4193 t
ORIGIN      
        1 tagaagtata tgtctagtat tgttgaattt tcaatgatat attaaggttc taatatatta
       61 tacaaaataa aaacattaat tcaacttaaa taattttaag tattatatgt gttttgatta
      121 aagaaaacta aaattttaag gaaaaataac atatttgtat taaattttat tgaaatgatt
      181 aaatttattt acttaatgta aattattagc taaaattgta ttaaataatt aaggaggtga
      241 gtagctctgg atgaaataga tttaaaaata attaattctc taaaaaagaa tagtagggct
      301 tcaacctctg aaataagcag acaaattaat ttatcagtac cagcagtctc agagcgtata
      361 aggaaaattg agcagtcaaa tttaattcaa aagtacacta ttcaaattaa tcgggaaaaa
      421 acaaaatata agcttttggc ttttatattt atcaatactg atttatctaa aagtatgaaa
      481 gaatttaaga aaactatatt ggaatatgat actgtattag aatgtcatca tatttcagga
      541 gagtatgatt acttaataaa agtattagca gaagatacaa gtagtcttga agagtttata
      601 tcagtgactt tgaaggaaat tggggggatt caaaaagcta atacaaccat tgttttatct
      661 acaattaaag aagaatttaa tacaataaaa gatatttttt aaataattta ctagtaaagt
      721 gttaattata ttttaggagg aaaatagaat atgaaagttt tagttttttt agcaaaaggt
      781 tttgaaacta tggaatttag tgtatttgta gatgttatgg gatgggctcg taacgattat
      841 gggcatgata ttgatgtagt gacttgtgga tttaaaaaac aagttatgag tacatttaat
      901 atacaagtat tagttgataa gacgatagaa gaagtatgtg ttgatgatta tgatgctcta
      961 gctattcctg gaggatttga agaatttgga ttttatgatg aagcatatga ttcatctttt
     1021 ttaaatctaa taagggagtt taattcaaaa gaaaaaataa tcgcttctat ttgtgtggca
     1081 gcattgccag ttggaaaaag tggtgtttta aaaaatcgta aagctactac atatcatttg
     1141 aaaaatggta aaagacaaag acagttaagt gagtttgatg taaatgtagt taatgaacca
     1201 attgttgtag ataaaaatat tataacctca tattgtccag aaacagctcc acatgtagct
     1261 tttaagcttt tagaaatgtt gacatctaaa gaacaaatgg atgaagttaa attagcaatg
     1321 ggatttaaac tgtaatggtt tatagatttt ctaagtaatc agaaccccag tcacacatgg
     1381 aatctaaaat tggaataata ctctttccaa aatcagatag cgagtattct acttttggag
     1441 gaacaacagg ataaaccact cttataatta aaccatctga ttctaattca cgtaattgtt
     1501 gagttaacat cttaggcgtt gctttaggaa ttaatttttt taattcattg aacctcaata
     1561 tagtgtcttt aagatgccaa agtataagtg tcttatattt accaccaatc aatgcaagtg
     1621 ttgcttctat tgggcaactg taattatcta aacatttttt catttttggg ctcctttatt
     1681 tattttaaag tatcttttta ggtactatat tacaaaaaag tgcattattg taatatagta
     1741 ccatagtgat aaaattatag cataggttaa ataaatcatc aacttagcct aataaaacaa
     1801 tagggggtaa ataaatgaat ttcgttgaac ttgcaaaaaa aagatattct tgccgtaatt
     1861 atcaagatag aaaggttgaa aaagaaaaac ttgagaaggt tttagatgtt gcaagaatag
     1921 ctccaactgg tggtaatcgt caacctcaaa gattgattgt tattcaggaa aaagaaggta
     1981 taaataaact ttctaaggct gccaatatat atgatgctcc attagcaatt cttgtatgtg
     2041 gagataaaga caaagtttgg acaagacctt ttgatgggaa acaacttaca gatattgaca
     2101 caagtatagt tacagaccat atgatgttac aagcaactga acttggattg gcaagtgttt
     2161 gggtatgcta ttttaatcca gatataatta gagaagagtt tagtttgcca gataatctag
     2221 aaccaataaa tatattattg atgggatatg aatctaagat accagagagc ccagagagac
     2281 atgaaaaaac tcgtgttcct ttaagtgaaa ttgtttctta tgaaactctt taattataaa
     2341 attaagttat tcataggata aatgtaataa agtgtttatt ataaacaaaa ttcaaaataa
     2401 ataatatcta catataatta attttagtga agtatatcta gtttaactag aatgtatact
     2461 tcactaattt atttattcaa aatatttatg aggattttaa tatattaact aaaatatatc
     2521 aattgaatta acttttaaat ctggaaaagc acttgaaata tagtgtttta tgtcatttaa
     2581 atggtcttga atttgctttt ctgatatttt atcaggtcca tatacaaaat acataatatt
     2641 tttagtatct tttgttatag ctgttcgatt atcagggcca ttaagaatac tagataatat
     2701 accaatatta tctgataaaa ataaatcatc ttttgttaaa atatgttctt taccacctat
     2761 accataaaaa tgttcacttc catttgctaa ttctattttc aaaggtaatt ccattttatc
     2821 aaggtcatga cctgctgtta gaagaagatt tttcaattct gctaaaaaca ttgactcaat
     2881 tgttacacct acatctggga taccttttga ttttaataga atagattcta attgaagcaa
     2941 tacagggtaa gttttcttga attttttata atactttata taacttgaaa caggctctgt
     3001 tttaattttt tctttacgat tgtattctgg gtgttgttct ataatatttt taattgtaga
     3061 gtttttaaga actaaaaaat tttctttatt aggttttgaa tgtgtaatat ttataaccat
     3121 aactccaaat ttaatattgg gataaatttc tttaacttta tgtgatattt ctaacataat
     3181 atagactcct ttttatttta tattttatga tataatctta gcaaattaaa agtggatttt
     3241 gtagttccat ttttatagta attttataga accacttttg gaggtattag aatgtggatt
     3301 acgattaata gaaatagtaa tatatcatta tcaagacaaa tttattgtga gataaaagat
     3361 atgattctgg atggaagctt aaagtttgag caaaaactgc catcatctag gactttatca
     3421 aaagaactat ctgtatctcg taatacagtt ttagatgcat ataatcaatt aattgcagaa
     3481 ggatatttgg aaacaaggaa tggatatgga acaattgtag ctaaaggaat atcagggcat
     3541 aaagttgatc ttaagttaaa tgatactttt agagatgaaa aagatttaca tactaataaa
     3601 caaaaaaatt atatagattt tcgttcagga gttccagaat taaaactatt tccacgcaaa
     3661 gaactttcaa agctatataa acaaatactg tatgatttat cagattcgga tttcagatat
     3721 ggaagtacag ctggtgtatg ggttttaaga gtggaaatat ctaagtattt atttcgtact
     3781 agaggaatta gatgtaatcc caaaaacatt atgattgttt ctggttcaac acaaggttta
     3841 tctttaattt cacagctttt atacaaaagt aataaaaagg ttgctgtaga agaccctatt
     3901 cataaaggct tattaaaggt tatttcatct attggatatt cagttgttgg aattaaagta
     3961 gatgataagg gaatgaatac caacttaata aaatcaataa aggggatttc ttttgtttat
     4021 actacacctt ctcatcaata cccattagga gggattttac ctattcaaag aagactagag
     4081 cttattaaat gtgcaacaga gaatgattac tatataattg aagatgatta tgatagtgaa
     4141 tttagatatg agggtcaacc aattagttct ctttatgaac ttagcccaaa taaagtaatt
     4201 tatataggtt cttttagtaa aatattatct ccagctattc gtgttggatt tatgttatta
     4261 ccagacaata ttttagaaag ctataaaacc ttaaaaatgt actctgatgt tcatacagaa
     4321 acaatattgc aacatgtatt agctggattt attggaaatg gaggtttaga gaagtacata
     4381 tttagaatga agaagttgta caatagaaaa cgtaagtacc tcataaaagt tttaaacacg
     4441 gaatttaaaa atgaatttaa aataaaaggt gaagcagcag ggcttcatat agttgctcat
     4501 ttctataatg ttaaatttac ggaaagttta attcataaaa tttttgaagc aggtataaaa
     4561 gtttatccta tacaacaata tgcaattgag aatatattag aatatgacca tgaaattata
     4621 ctaggttatg ctcatcttag ttttgatgaa attgaaaaag gtgtaaaaat actcagcaaa
     4681 ataatacata tgaatcaagg tacatagata aaacatgtag caatctacat ataatagatg
     4741 taattaagtt taaactttaa aagaaatata tattaatatt tttgtataaa aatacctgat
     4801 atttttttat cgatacttta ttgctataca ctaaattaag atagcaattt atattaagaa
     4861 aaaacttttt gcataggtta taatacttaa aaggagagga tagagttgaa aacaaataca
     4921 gatgttatta tggagataat tgattatata gaagaaaatc ttacagaaaa tctaaattta
     4981 gataaaatag cttatattgc taattattca aaataccatt tacacagaat gtttacaaat
     5041 gttgtaggat gtactatcca tcaatacata cagaggagga gacttactga agcagctaga
     5101 caacttatat ttacagacaa atatattata gacatagcta ttacagtagg atattctaca
     5161 catcaatctt ttacactagc atttagaaat ttatataaag aatctccaca agtatataga
     5221 aataaagatg aattttatcc tattcaacag agatttgttt taaatctaaa tcatcaaatt
     5281 caaagcgaaa aatgtattta ttatgcaaat atgaattata aacaaagaag agatgaaatc
     5341 aattcttatc ttaactttaa tattaattgt gaggtgaaag tagcatgagt ttagtgccac
     5401 atgtaattga acaaacaggt caaggggaaa ggtcttatga tatatattca aaattattaa
     5461 aagatagaat tatatttata ggagaagaaa ttaatgatgc tattgcaagt ttagtagtag
     5521 ctcaattact gtttttagaa tcagaagacc cagattcaga tataattata tatataaata
     5581 gccctggtgg ctctgctaca tcaggatttg ctatatatga tactatgaat tatgtcagat
     5641 gtgatgtatc gactatttgt attggtatgg ctgcaagcat gagtgctttt ttacttgcag
     5701 gaggaacaca tggaaaaaga tgtgctttac ccaattcaga gattatgatt catcagccaa
     5761 tgggaggagc taaaggtcaa gcaacagatg taaaaatagc tgtagataat atattgaaaa
     5821 taaaagaacg attagataaa attctttctg aaaatacagg taaatcaata gaagatatta
     5881 ggagagatac agatagagat aattttatga ctgcactaga ggctaaagag tacgggctga
     5941 ttgattatat aatgaatagg aatgaataaa gtatcaaagt atcatattaa aatgtctgtt
     6001 ttacaaatat atatataata ttaaataaga gtgtctcaaa atgaactttt taatttatgg
     6061 gacactcttt tttgtatgaa attaaatatg gttttatcat ttcaacaatt attaccaaat
     6121 ttcaagttta ataaattcta ctataaaaaa tacataaaag ataaggttct ttagtaactt
     6181 tctgtcgtca aaagttttta taaatttaat aacaaaacaa gagaaaatac catatattac
     6241 ttatgtctgc aatatataga agacaaagga gttgtacatt atgattacaa taagtttgtg
     6301 catgattgtt aagaatgagg aagaagttat aggcagatgc ttagaatgtg taaaggatat
     6361 agtagacgaa attataattg tagatactgg ttctacagat tccacaaaag aaatagtctc
     6421 aaaatataca gacatggtgt acgatttcga atggatagat gatttctctg ctgcaagaaa
     6481 tttttcattt tctaaagctt ctaaagacta tactatgtgg cttgatgcag atgacataat
     6541 tttagaagct gatagagaaa aattattaaa gattaaagaa agcttggata cttccattga
     6601 tattgttatg gcaaaataca atgtatcctt tgatgaaaat ggtaacccaa ctctctcgta
     6661 ttatagagaa cgattgttta aaagaagttc taatcataaa tgggttgacc ctattcatga
     6721 agtagtacct ctttttggag aggtatttta ttctgacatt gcaatatctc ataaaaagct
     6781 tcatagacag gaccctctaa gaaatcttag aatttttgaa aaaatgattt cagaaggaaa
     6841 aacattggag ccaagacacc aattctatta ttctagagag ttgtactata atgctagata
     6901 taaagaagca atagaaggtt ttactaagtt tttagactca tcaagaggat ggattgagga
     6961 ttgtatcagt gcttgcagag atttagccac atgttattac ttaattaatg atgaaaaaag
     7021 tgccttatat tcattgttta gaagtttcga atttgatgag ccaagagcag aaatttgttg
     7081 tgacatagga aagcatatgt ttgatagaca aaaatataaa gaagccattt tttggtataa
     7141 ggtagcatta acaagagata agaatgatac taatggaggc tttaagtcaa atgattgtta
     7201 tggatatata ccatctatac aattaagtgt ctgttatgac aggttaggag aatcagataa
     7261 agctatttat tatcatgaaa aaacaaaaga aatcaaacct aacgatagtg ctgttttgca
     7321 caatgaaaat tattttagta aatttaaaaa tagttgatag gtcacaatca tactaaattt
     7381 gacatattat atatagagct taaaagctca aatatatcag gaggtgcttt taataatgag
     7441 tagaaataaa tattttggac catttgatga taatgattac aacaatggct atgataaata
     7501 tgatgattgt aacaatggtc gtgatgatta taatagctgt gattgccatc attgctgtcc
     7561 accatcatgt gtaggtccaa caggcccaat gggtccaaga ggtagaaccg gcccaacagg
     7621 tccaacgggt ccaacaggtc caggagtagg ggcaacaggc ccaacaggac caaccggtcc
     7681 gactggtcca acaggaaata cagggaatac aggagcaaca ggattaagag gtccaacagg
     7741 agcaacaggg gcaacaggcc caacaggagc gacaggagca acaggagcag atggagtaac
     7801 aggtccaaca ggtccaacgg gagcaacagg agcagatgga ataacaggtc caacaggagc
     7861 aacaggggca acaggatttg gagtaacagg tccaacaggc ccaacaggag caacaggagt
     7921 aggagtaaca ggagcaacag gattaatagg tccaacagga gcgacaggaa cacctggagc
     7981 aacaggtcca acaggggcaa taggagcaac aggaatagga ataacaggtc caacaggagc
     8041 aacaggagca acaggggcag atggagcaac aggagtaaca ggcccaacag gcccaacagg
     8101 ggcaacagga gcagatggag taacaggccc aacaggagca acaggagcaa caggaatagg
     8161 aataacaggt ccaacaggtc caacaggagc aacaggaata ggaataacag gggcaacagg
     8221 attaataggt ccaacagggg caacaggaac acctggagca acaggtccaa caggagcaac
     8281 aggcccaaca ggagtaggag taacaggagc aacaggagta acaggtccaa caggggcaac
     8341 aggggcaaca ggagcaaatg gattagtagg cccaacagga gcaacaggag cagcaggaac
     8401 acctggagca acaggtccaa caggagcaac aggcccaaca ggagtaggaa taacaggagc
     8461 aacaggggca acaggagcga caggtccaac aggagcagat ggagcaacag gtccaacagg
     8521 agcaacagga aatacaggag cagatggagt agcaggtcca acaggagcaa caggagcaac
     8581 aggaaataca ggagcagatg gagcaacagg tccaacagga gcaacagggg cagatggagc
     8641 aacaggtcca acaggagcaa caggggcaac aggagttaca ggagcaacag gcccaacagg
     8701 agcaacagga gcaacaggtg ctagtgcaat aatacctttt gcatcaggta taccactatc
     8761 acttacaact atagctggag gattagtagg tacacctgga tttgttggat ttggtagttc
     8821 agctccagga ttaagtatag ttggtggagt aatagacctt acaaacgcag caggaacatt
     8881 gactaacttt gcattttcaa tgccaagaga tggaacaata acatctattt cagcatactt
     8941 cagtacaaca gcagcacttt cacttgttgg ttcaacaatt acaattacag caacacttta
     9001 ccaatctact gcaccaaata actcatttac agctgtacca ggagcgacag ttacactagc
     9061 tccaccactt acaggtatat tatcagttgg ttcaatttct agtggaattg taacaggatt
     9121 aaatatagca gcaacagcgc aaactcgatt cttactagta tttactgcaa cagcttcagg
     9181 tctttcatta gttaatactg tagcaggata tgcaagtgca ggaattgcaa taaattagat
     9241 tagataagaa aaggcttcta atatcgtatt gatattagga gcttttttaa gaatgattga
     9301 tgaacatagt tttaatatta attagtgcgc atattataaa atactattat ataatataaa
     9361 ataataacat taaattggaa atatagaatt atctaggggt gatactgtga aaaataagat
     9421 tgaaatatca atgaacaact ttgtaaaaaa ttatcataaa tcaaataata taactacaaa
     9481 gtggcaagaa cttttgtttg cttatgcatc tgctgaagat gatatgtttc agaagctaaa
     9541 aagtgttgta ggaacatctc atgctatgcc aaaagatttt ctggaaaatg gaaaaactat
     9601 aattacatat tttatacctt ttgatgaaag tgtaaataat agtaatattg gagggtttga
     9661 atgttctgaa atatgggcaa gagcgtacat agaaactaat attttgattt caaatttaaa
     9721 tgatttttta tgtgaagagc tagagaagat aggggctaat tcatatgcta ttcctgggtt
     9781 gtataatttt gacagtgaaa aattggtaag tgactggtca caaagacatg tagcattcat
     9841 agcaggtctt ggaacttttg gtttaaataa tatgcttatt acagaaaaag ggtgttgtgg
     9901 aagaatagga agcataatca caagtttaga attagagcca actaaaagac cagaaaatga
     9961 atactgcttg tataaacata ataatagttg caaaaaatgt gtcaacaaat gcgtgaaaga
    10021 agctttaaaa gaagatggat ttaataggaa taagtgttat gaaatgtgta tgataaatga
    10081 aaaaaaatac acttatatag gtggatgtga tatttgtgga aagtgtatag ttggagttcc
    10141 ttgttcagta ataaacccat caagataatg gatttaagtt gactaataat tttaaagata
    10201 gtttataatg aaaaaatttt agttataaaa ataaatataa tataaaaact aaataaatgt
    10261 gtttaaaaaa tggataaata atttgtgttg attgacaacc agttatactg ttgatagact
    10321 tatatgtatc gctgtgatat ataaggaggg ttgagatttt atgtttgatt taatctttat
    10381 ttttgcatag caagggctat tgcaactatg aaatttaata tataaatagc cctttgctat
    10441 tcctaatgaa aaattactat ttaataggag agcaaaatga gctataaaaa agcaatacat
    10501 atattaccag aagaattact tgaattaata caggaatatg tagatggtga atgtatctat
    10561 attcctagaa aatctaataa caaaaaagaa tggggaagta atacacctac tcgtgaagag
    10621 ttgtcaatta gaaatatgca gatttacgaa gactatcaag ttggatatga tttgcaatat
    10681 ttatcggaaa agtattattt atctttgaaa agtatccaaa gaattgtatt acaagaaaaa
    10741 agaaaagata tctaaggatg agccagtata gttttataat tatactggct cattttcttt
    10801 tgataaaagt gtttgaggta tagtttcttt taagtggatg atataattta agtaatttca
    10861 ataacttata cagtagctaa caaagctggt tgtaaagtat ctaataagaa gtagtagctt
    10921 tttatatgtt tgaaattaaa atttattaaa attaggaggt cttgttttga aaaaaatact
    10981 tgattatatt ccaccttttc ataactttct attaatttag taatcttata gaaaggaaat
    11041 tgcatttatg aaaaaaatta cttgggaggt ggaatatatc tcccagttac ctgtaattag
    11101 gtattgtaag aaatgtggaa aaaagactga atatatttgt tcaggtttat ttagggttaa
    11161 tgctcaacat aaatatttgg atatatggtt aatatacaag tgttcaaatt gtgattcaac
    11221 atggaactca actatatatt ctcgtgttaa tccaaaaagc ttaaatccag aaatactaga
    11281 acaattccat acaaacgatt ataatcttgt taagcaatat gctatgaata tggaactttt
    11341 acatagaaat ggagcagaag ttggtattcc agagtataaa atagtaggac aagaaattaa
    11401 tattaatgac cttacacaag ttcatattat tagtaaatat ccatgtcagt taaaggtatc
    11461 aacattatta cgtgagaaac taggtttatc taggaaggta tttgacgaaa tgttggattg
    11521 tgggattatt agaagtaact caggagttga tttaaagaaa ggtaaattaa attatgaaat
    11581 tatgttagat ataggcaagg agaagcaaaa gtcttcaaat tgattagaat aactaactta
    11641 atatattttc agtaacatca tttaaaaaaa tagtgacttt aaaaagttta aagccactat
    11701 tttttaatat gttttatatg agtatttaaa ataaagtgtg tattcttcaa atttatagcg
    11761 ataattaaaa cattactata aaaaggaagg tgcatacttt ttatttaaca aaaactagat
    11821 attaaaaact tttcgaggaa tattttatta aaatccctaa atatatttaa tatatattaa
    11881 aaaattgtat tataatgaaa atcaaaatag attgcaaatc caaaaagtaa tacttaatgt
    11941 ccaaaaagct aaaattaaaa tctaaatagc tatttactac agaagtatgt taaaatatgt
    12001 aaaatgaata gaaatttata tatttttatt aaaaatatat aagaaaggat gtttaagaat
    12061 tgtgattaat ataggaatat gtgatgatga actacattat cgaataaaaa taaaagatat
    12121 attaagtgaa atattaagtt catatcctat aaattataac atatatgaat tttcagcagg
    12181 agaagagtta ctaaataact atcctaaaga tttagacata ttaattatgg acatacaaat
    12241 gaaaacaata aatggtatgg atactgctag gaagataaga gagtttgatc ataagttaga
    12301 aataatattt gttacttcat ttgtagagtt tatgcaagaa ggttatgagg taaaagcata
    12361 cagatatata ctaaagccaa taaataaaga aaaaatttct aaaagtgtat taccttgtat
    12421 aaatgagatg atgaaaaaga gaaataatta cttaactata aatgtaaaaa attatgtaga
    12481 ccgaattaaa atagattcta ttacttatat agaaacagat agaccgaata tattgattta
    12541 tacacatgat gatatgtata ttactaaaat gagtatatct aaaatagaaa agatattaaa
    12601 tgaatatgga ttttttagat gccataatag ctatatagtt aacttaaaat tagtagaatc
    12661 aatgagtgga agtactgtta tagtagatgg aaaaagtatt ccaataagta aatatagggt
    12721 taaaggcttg aagcttgcga taactaatat attaggggac atagtatgct aacaaattta
    12781 agtgtaggat tttggaatat tattaattta gtttcaggta ctatagattg gattatattt
    12841 tttttagtaa tccacttagt tggaaagagg aaaatatcta atataagata tatatttaca
    12901 acaataatta taattatatt tatggcatac ataaatgtag ttaaagtgtt tccaaatatt
    12961 aagattcttc tatgtatggt aataggaata tcattttata taacatcata tgaagataaa
    13021 atctataaaa gtataatagt tagtttattg ttttggctag gattaatggt tgctgaaggt
    13081 tctgcagtag gtctagtagt atttataaat aaattaaata atattaacat aacacaacat
    13141 gaaaacttat ttagaataca agctattatt atgtcaaagg tatttttatt tataggtttt
    13201 atattgttta agtattt
//
LOCUS       DAEMHW010000104        13046 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEMHW010000104 DAEMHW010000000
VERSION     DAEMHW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13046
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.104"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            36..1895
                     /locus_tag="KPK67_002433"
     CDS             36..1895
                     /locus_tag="KPK67_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2459389.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLIKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIKNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2187..2786)
                     /locus_tag="KPK67_002434"
     CDS             complement(2187..2786)
                     /locus_tag="KPK67_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2459390.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2889..3686)
                     /locus_tag="KPK67_002435"
     CDS             complement(2889..3686)
                     /locus_tag="KPK67_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2459391.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3799..3904)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3996..4982)
                     /locus_tag="KPK67_002436"
     CDS             complement(3996..4982)
                     /locus_tag="KPK67_002436"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG2459392.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5466..7196
                     /locus_tag="KPK67_002437"
     CDS             5466..7196
                     /locus_tag="KPK67_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBG2459393.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7306..7386
                     /locus_tag="KPK67_002438"
     tRNA            7306..7386
                     /locus_tag="KPK67_002438"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7340..7342,aa:Leu,seq:tag)
     gene            7721..8236
                     /locus_tag="KPK67_002439"
     CDS             7721..8236
                     /locus_tag="KPK67_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBG2459394.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKI
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVNCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNM"
     regulatory      8712..8816
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9026..11500
                     /gene="gcvPA"
                     /locus_tag="KPK67_002440"
     CDS             9026..11500
                     /gene="gcvPA"
                     /locus_tag="KPK67_002440"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBG2459395.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGTESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEIEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11500..12957
                     /gene="gcvPB"
                     /locus_tag="KPK67_002441"
     CDS             11500..12957
                     /gene="gcvPB"
                     /locus_tag="KPK67_002441"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBG2459396.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDPDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         4890 a         1562 c         2160 g         4434 t
ORIGIN      
        1 aacataaaaa acaatatagg ggataaaggt gttttatgaa gagatttttg aggagaatta
       61 tcttagtttt atttattttg ttgattttta taagttttat ttctattaaa ttaattcata
      121 atgttggaga ttatggaaaa ttgattaatt atgttgggat tgttcgggga gctagccaac
      181 gtctaattaa actagagatg aatcatcaac caaatgatga attaatagaa tatattgatg
      241 aaatcctaca ggagttgatt acagggcatg gagattatgg tttagtaatc actgattgta
      301 atgaatacaa tgaggactta cttttattag aaaaaaagtg ggaagattta aatattgaaa
      361 ttaaaaaagt aagaatgaaa gaacagaata accagttatt aagtataagt gaagagtttt
      421 tttctcttgc aaatgataca gtgtttaaaa tagaaaactt ttctaaagaa aaaagtaatt
      481 acttaatgac tttaataata attatatcta ttataggtat attagcatgt atcatattga
      541 ttttacaata tagtaaaaaa atggttagac tggaaaaatt aaatgtagat ttaaaaaata
      601 ttgcatataa agatgaacta acaggagtaa atactataga aaaatttaaa ttggatgcaa
      661 atcaaaatat ttgtatacat aaggataaga aatttgctgt attctatatt gattttgaaa
      721 actttaaata cattaatgat atatttggat atgattatgg tgacatgata ctgaaaagat
      781 atgctaatct tatgatgaat gacataggga aatatgaaat atttgcaaga gaaattgcag
      841 atagatttgt tgcgttaaga tgctatgtag ataaggaaga tttagtggta agacaacgag
      901 atgtagacaa tgaactaatt aatataacaa atgaaattaa aaataaacat agtattactg
      961 ttgttagtgg aatttgttgt attgaagatg taaatgaaaa attaagtata gatggtttaa
     1021 taaatagagc aaattttgca caaaaaactg ttaagaataa accaggtact aattatgctt
     1081 tttataatga cagtattcgt aaaaaaatga ttgaagaaaa tactatcaag agtagaatac
     1141 atgaagctat tgaaaaaaga gaatttatag tttatttaca gcctaaagtt aatttacata
     1201 gtcaaaaaat aaattgtgca gaagctttag ttcgttggct tactccggat aaaggtatta
     1261 tatcgccagc aatatttatt ccagtacttg agaagaattt ttttattgcc ttagtagata
     1321 agtatgtttt tgaggaggtt tgtaaatgga ttagaaagag gttagatgaa aataaacctt
     1381 ttgttcagat atcagttaat gtttcaagaa tacaatttta taacactaaa tttgtagaaa
     1441 cttattccaa tataaaaaat aaatacagga tacctaaaaa tactatagaa attgaattta
     1501 cagagagtgt tgcttttgag aatcaaaaac atcttttgga aattatacat gatttgcatg
     1561 aaaatggatt tacttgttct ttagatgatt ttggaaaagg ttattcgtct ttaagtgttt
     1621 taaaagattt accatttgat gttttaaagc ttgactctat gtttttcaag gctagtttag
     1681 ataaagataa agagaaaata gtaataaaaa atattgtaca tatgttaaaa gaacttaata
     1741 tcacaactgt tgctgagggc attgaatgca aggaacaagt tgaatttctt agggatattg
     1801 ggtgtgattt agttcaggga tttgtatttt ataaaccaat gccaatatta gaatttgaaa
     1861 aaatattgga taaagagttt gtttataata gctaaaatgt attttataaa atatgaacta
     1921 attaagtaaa attattttgt actgaataaa tttttacttt atttacagaa taatgattat
     1981 ttacagaaaa gcgcgaaaag tgtattcctt caagtgtggg atggatagca caaatagggg
     2041 agaaaaaata ttggccatat aagaatgcat aataatataa atatttgaaa ggtcagcatt
     2101 atataaaaat taaaaagaaa ataaataatt tttctataaa aaagagacta tgagaaaatt
     2161 tactcatagt ctcctaaata atgtatttat tttcttcctg caacccattt taatccccaa
     2221 tgaaattcag tatccatttc tctatgacct ttgaagtatt taactacttt ttcaacactg
     2281 aatgtctcat catttacatt ttctatcaat gctattgaac acatattata gccattttta
     2341 tgttcatcaa gtttgacaac taagtctgaa tcttgttttt gtttgattgt tacaattcca
     2401 tcagcttcag accagttagc tacaccttcg tatataaaag cataaataag tattcttttt
     2461 atatctttta ttttattacc atttattctt aaattttctc cttgtgtatt acttcctgtt
     2521 ctgtcatctc catctaattg agcaaaaggt ggattgtcta aactaccaaa ggcatttcct
     2581 aatgcttgaa cagcaccttt tacaccattt ttcatctcaa ataggcaacc caaatctaaa
     2641 tctatattac tgcttcttaa agagccaaaa aaaccctttt tttgtacttt ttgattccaa
     2701 tttaaattaa ctaagatttc acctaaatcg ctattttcct tttttgtaag gtttattttt
     2761 tgtccctttt ttaattctat agccatagta atcttcctcc acaaacatag atttatttaa
     2821 atggtagcac ttaataagtc aatattcaac aagattagaa taataatatt aaaattatac
     2881 taaatatact aaaaagtagg aatcacaata cctttgtatt tttcttcaat taagcttctt
     2941 gctttatctg atgtaagaac atcttgtaga actttaatct tttcactgtc tttatcttca
     3001 gttctaaatg ctaaaagatt tgcatactcg tcatttatag gagctgtata aattccgttt
     3061 ttcttggcat caagaccagc aggtacagca taagtagtat ctataaatgc agcagtcaca
     3121 tctggaagag aagatggaat ttgagcaata tctaactctt tgaattgtat tccctttgga
     3181 ttttctatta tgtccttagg actaactaat ccatcccctt taggtaacgt taaaagtcca
     3241 atactttcaa ggtatctaag gttcttagaa caagaagatg ggttattgct tattgcaatt
     3301 gtatctcctt ttttaaattc atcaacagat tttatcttat aagagtaaag tattgctggg
     3361 catacatata gttttttacc agccattatt ttgtaacctt tggccttaac agcttctttt
     3421 agataaggtt catgttgaaa tagatttgca tcaatttcac cattatttaa agcttcgttt
     3481 ggaagtatgt aatcatcaaa gtttttaact tctaaagtat atcctttttc tttaataagt
     3541 ggttttaatt cttctaataa ctctcctcct ggaactaaag tagcaccaac aacaattttt
     3601 ttatcatctt tagctttaga acatccaacc acagctaaag ttaatactag acataataac
     3661 aaacataata attttttgaa tttcatataa attccctcct gaatataatt gattagttaa
     3721 aaataaaata ccattcttta tcacactgca aaataaaaat aagcctgagt ttaaactcag
     3781 gctttaaaag ctaatttaga ctcatctttc agtataatac tgcaggattt agcaccaatg
     3841 tatataaaaa tatactggtt gctgggtttc atagggccag tcccttcacc actctggata
     3901 agaagtatca atttttaatt atgttataaa tattatcatg attttttacg tctgtaaaca
     3961 taattttata aatattttaa aatctttaat gtattttatt taacacctaa aattccagaa
     4021 actagaatat cagtatcagc acctaagaaa tagtcattta tttcaataga atccagagta
     4081 tttctcaaag cattttcttc atgccttata ccaataagtt tattttctac aaatgcaaca
     4141 tcacattttc caaaaaaatc tccaaaaaat tttatactag ttattatgcc tttatcaaca
     4201 tttaaattaa actcaacatt tccaccagaa tattttaatt catttgaaag agtatatttt
     4261 ggtgaattac caaaattcca atcccaagtt tcgtattttg atttagtaag cttttctatt
     4321 tgttttatat catgctcact caaacaataa ttagtattat cagtatttgt tttagaaaga
     4381 taatccatta tagcatcttt aaattctaat atgtccattt ttgaatgtaa atgttcactt
     4441 atattggtta cacgagattt tacagatttt attccttttc cttcaaattt tataggttta
     4501 acctttaatg cttttgagag gtcatttatt tcagatgaaa ataaaagagt accatgatgc
     4561 attactttgt ttttgtagtt gtattgagca tttccagaaa atttcttgcc atctataaga
     4621 aggtcatttc ttccagaaaa ttcggcattg acatctagtg ttttaagtaa atctattatg
     4681 ggctgagtga atcttctgaa atcactaaaa ttattattgt tacaagctat aaatgtaaaa
     4741 tttatatttc ccaaatcatg aaatacagct ccaccaccag actgtctcct aacgacaggt
     4801 atagaatatt cttttatata ttctaaattt atttcagaaa gtgtgttttg gttttttcca
     4861 acaattatag aaggttcatt cctccataaa ataaataagt cttcatcaaa attttttaga
     4921 agatattctt ccattgctaa attaaaataa gggtttgttt tttcattata aattaataac
     4981 attaaagtag ttcctccata taattgataa aatttttatt atgaatacaa ttaattttat
     5041 aattcgacaa taatataaaa actcctttaa attagaaatt ttaaaaacta ccttgcaaag
     5101 tttaaataaa taatatatac tcaaaatatg tttaataaaa gtgaatagaa gggtagaggc
     5161 gcaataatct atcagtacta ataaggagtt aagcacagag agttattagg aaaggagata
     5221 ttgccgaagt tttataatcg atgctttaag attatattgc tgggggtaca tagaaaatat
     5281 gtacaactgt cacaggttta tttgtggtga gctatcattt aaggaattat attatattta
     5341 tttttactat tgttgatata ttgtaatgtt taagccttga gtgtttacca ctcaaggctt
     5401 ttttagtttt attgtaacat aataccctgt acagttcaca aaattaaaag gagagggaga
     5461 tttatatgag gaacaaaaaa attaatatta tttttctgac gacaatcatg ttcataatgt
     5521 caacagtaat ggtttttgca gaagaagata tagacactat tgcactagct aatgcagaaa
     5581 aatttgggat tttaacttta ataccaccat tagttgctat aatacttgca tttataacta
     5641 aaaacgtaat aatttcttta cttataggta ttctttctgg tagttttata attaaagcta
     5701 gtggtattaa tgtttttgcg acatttatac aggcattttt agatttagta gatagagctt
     5761 tagtatcttt agcagaccct tggaatgcgg ggataatatt acaggttttg gcaattggag
     5821 gagtaataaa tcttgttgct aaaatgggag gagctaaagc catagctgaa gcacttgcta
     5881 aaagagcaaa gtcagcaaaa gggacacagc ttataacatg gtttttgggg cttttagtat
     5941 tctttgatga ttatgccaat tcactaatag taggtcctat gatgagacct gttgcagata
     6001 aaatgaaaat atcgagagaa aagctagcat ttataataga tgccacagca gcaccagttg
     6061 ctggtttagc tataatatct acatggatag gtcttgaggt tggtttaata catgatgctt
     6121 ttgaaagtat aagtatagat gtagatgcat ttggtatatt tttaaataca ataccattta
     6181 gattttacaa tatattaata ttagctttca ttgtaatatc agcattatta ttaaaagaat
     6241 ttggtccaat gagaaaagct gagataaaat ctagaagtag aaaaattagt atagaccttg
     6301 atgaaggcgt agaagagttg gacgatttag ctccaaaaaa tggagtaaaa ttgagtgtat
     6361 ggaatgctat aatcccaata ggaacattga taatagttgc attagcatca ttttattata
     6421 gtggatatac atctataatg ggaggagacg ataaggctct gatacaacta tttacaaatt
     6481 ctccatattc ttttgaagca ataaaagaag catttagtgc atctgatgca tctagagcat
     6541 tattccagtc tgcacttgtt gcaagtttag ttgctattat aatggctgta gtaaaaaaga
     6601 tttttacaat atcagaagca attgatgttt ggatagatgg aatgaaaagt ttagtaatca
     6661 ctggtgtaat attaatactt gcatggtcat taagttcagt aataaaagag ttaggtacag
     6721 ctaaattttt aatacaccta ttatcaggtt cactaccacc attcttatta ccaagtttaa
     6781 tatttggttt aggagcaata atatcttttg ctactggaac agcttatgga acaatgggta
     6841 tacttatgcc acttgcaatt ccattagcat actcattaaa tcctgatatg tcttatgtaa
     6901 tagtaagtac tagtgcagtt ttaacaggtg caatatttgg agaccattgt tctccaattt
     6961 cagatacaac aatactttct tcaatgggag ctggctgtaa tcatatagac catgttaaca
     7021 ctcaaatgcc atatgctata tttacagcag taatcactat tgtatttggg tatataccag
     7081 ctggacttgg attgccaata tatatagtat taccagtggc aatagctgct atatttgtag
     7141 gtattcaaat aattggtaag aaagttgatg aagcggaaat agaacttgta gaataaagat
     7201 ataaataagt ttcacctttt attatcacta ttttgttagt ttttagataa ttttaaaaag
     7261 ttattgaaaa taatggcgaa atatagtaaa ataaaagagt gataagcgga tgtggcggaa
     7321 ttggcagacg cactagactt aggatctagc gccattggcg tgggggttcg actcccttca
     7381 tccgcacttt acagtactta attgtactga aaaaaactgg ggttgctggc tttcggagtg
     7441 ggctatcccg tttttatttt tttgtatata taatagattg gcactttagt ataaggtgct
     7501 ttttttatag gcaaaaatag tacatttatt tctgttttat tatcattcat ctattagaat
     7561 atactttagt taagaatgtg ttaataagca gtgttcacat tcttgataaa atgtggaata
     7621 atatgtattt ttactaagac ggaaatgaaa taattaaacc attaaataat attataagat
     7681 gatgaataag tagataaatc aaattttgta ggaggatatt atggaaaaat atatatggtt
     7741 gtttccatta ctttttattt ttcatgatat ggaagagata ataggttttg gaatatggct
     7801 taaaaaaaat aaatcaatgc ttgataagaa gtatccattt atcagtaaaa tttatgaaaa
     7861 ttatagtacc gaaggcatgg catttgctgt gtttgaagaa tttattttat gcattatttt
     7921 ctgtatactt acagttataa cagagaatca gtatgtatat ctactatggt taggtagttt
     7981 tatagcatat accttacatt tggtaattca tatagggcag tcaatcataa taagaaaata
     8041 tataccttca cttattacca gtatcatatg cttaccaata agcatttggt gtataagtaa
     8101 gagtatttat atagttaatt gtgaaatgag tacaacaatt ctgtacagta taattggcat
     8161 tataattgta gcattgaatt taaagtttgc acagtcgctg attggaaaat ttacaaagtg
     8221 gatgtcaaac atgtaaattt tagtttattg gacagttcaa ggaatcagaa attggaattt
     8281 tatagtatta atataatgta taaaatttta tagcaatgta tttcataagt ataaagtgac
     8341 tagaatttta agaaatatac aaattctata aaaactaaat aatttcaagt atattttaaa
     8401 aatcttattt ttcacaagtt taaaattgta aacgatttca tcaagtctat gaatatactt
     8461 ttaacatgtt atagtaaatc tcaatataaa tttacatttg gttctttgtg tgttatttta
     8521 ataataaatt aagtgaaaat gttattttac attaatatat aatatctttg cataagagtg
     8581 aataatataa aagagaaata attgataata aaaacttaac aaaaaaatta atacataaaa
     8641 aaaaataaat ataatgtgtt gacatttaat tgaaatattc atacaataaa attgaaaata
     8701 aatatttcca aatgagatta gcgagagagt cctttataca aaaaggcgcc gaagaagaaa
     8761 tttaagaaat gccaattttt agagaagctt tcaggcaaaa atatcgctaa ttgatgggac
     8821 tctggagaga cttaagttaa gatactataa gtcaccgaag aagcaaaata atattaaatt
     8881 attaaatctt tcaggtatac aaacagggat atataggcta ggattatttt ccatgctgat
     8941 atatcttttt tttatgaaaa agtacaggaa ataatatcaa agttacttaa atacagtagg
     9001 tctaattttc tagggagggt tatatatgaa tgaattgaaa agagtatctt tgtacaatat
     9061 acacaaagag cttggagcaa aacttgttga atttgctggt tgggaaatgc ctttagaata
     9121 tgagggtata aataaagagc atgagaaggt cagaaaaagt gcaggtatat ttgatgtatc
     9181 tcatatgggg gaggtacaga taaagggaac agagtcagaa aaatttatac aaaacctagt
     9241 tacaaatgat ataagcacat taaagataaa tgatattata tatacaccta tgtgttatga
     9301 aaatggtgga gtagttgatg atttattaat atataaattt ggtgaagaag attatttatt
     9361 agtaataaat gcaggtaata tagataaaga tgtagcatgg ataataaaac aaagtgaagg
     9421 atacaatgta gatataaaaa atatatctag tgaagtgagt caattagcaa ttcaaggacc
     9481 taaagcagaa gaaattcttc aaaaaatcac agatatagac ttaaactcaa taaaatttta
     9541 taagtcaatt ccatcaacaa aagtatgtgg ttgtccttgt ttagtttcaa gaacaggata
     9601 tactggggaa gatgggtttg agatatactg taaaaataaa tatgtagaaa taatttggaa
     9661 tgaagttcta aaggttggag gagaagatat atgtccagct ggtctaggtt gtagggatac
     9721 tttgagattt gaagcagctc tacctctata tggacatgaa ataaatgaac acatatctcc
     9781 aattgaaggt ggtttatcta tctttgtgaa aacaaataaa gaatcattta ttggcaaatc
     9841 aattttatca aaagaaaaag aaagtggagc taaaagaaag ttagtaggat ttgaaatgca
     9901 aggtaaagga atgcctagaa atggatatga tataaggatt ggtgataaaa cagttggatt
     9961 tgtaacaaca ggatgtgcat ctccaacgac aggaaaaatt ttaggtatgg gaattataga
    10021 ttctgaatat gcaaaagtag gaaatgaaat aggtatagca atcagaaaaa aagttgttcc
    10081 tgctgtgata gttaagaaac ctttttataa aaaacaatat aaaaaagata atattatatt
    10141 aaacaaagaa aataaatttt catatatacc tgctacaagt gaagataaat caaaaatgtt
    10201 aaaagtagtt ggattaaatt cggttgatga attattttca gatataccag aagaagtaaa
    10261 attaaaaaga gatttaaatt tggaaattgg aaaatctgaa ttagaggtaa gcaaaatagt
    10321 aaaaagattg tcagaagaaa atttaagttt agaagacttg acttgttttc ttggtgctgg
    10381 tgcatatgac cattatatac catcaattat aaaacatata acttcaagat ctgaatttta
    10441 tacagcatat actccatatc aagctgaaat aagtcaggga actttacaag tagtttttga
    10501 atttcaatct atgattgcag aaattacagg tatggaaatt gcaaatgcat caatgtatga
    10561 tggagcaact gctgcaatag aagcatgtat aatggcaatg aatcagacta gaaaaagtaa
    10621 aatagtagtg tcaaaaacta ttcatcctga aactttgtca gtattaagaa cttacttaca
    10681 atataaagat tgtgagatag tagaaattga cttttgtaat gaatatggga ctacagatat
    10741 agaaaaatta aaagcttctg tagacaaaga tacagcatgt gttcttatac aaactcctaa
    10801 tttctttgga attattgaag aaatagaaga aatagaaaag ataactcatg aaaataaggc
    10861 tatgttaatt atgagtgtag atccaatatc attaggagtt ttaaaaacac caggtgagat
    10921 aggtgcagat attgttgttg gagaagctca atcacttgga aaccctctta attttggagg
    10981 tccttatgta ggatttttag ctagcaaatc taaatatact agaaaaatgc caggaagaat
    11041 agttgggcaa agtttagatg tagaaggtaa aattgcatat gtattaactt tacaaactag
    11101 agaacagcat gtaagaagag aaaaagcaac ttctaatata tgttcaaatc aagctttaaa
    11161 tgctcttgta gcttcaatat atatggctac tatgggtaaa gaaggattta aagaggttgg
    11221 tatgcagtct atgaaaaaag cacattatac ctacaataaa cttgtacaaa ctggaaaata
    11281 taagccaata tttaaaggta agtttttcaa agaatttgct gtacaaggaa atcttaatat
    11341 agaaactata aatgataaac tcttagaaga aaatatatta ggaggttata atttagaata
    11401 caattatcca gaattaaaaa attcaacttt gctttgtgtg actgagaaaa gaagcaaaga
    11461 ggaaatagat aagttagttg gaataatgga gggattataa tgaaagaata caatagtttg
    11521 ttgatagata tatctaaaaa aggtaggaaa gcatactcac ttccaaagtt agatatagat
    11581 gatataaaaa tagaagatat gattgaccca gatatggcaa gacaaagtga gttgaatttg
    11641 ccagaagttg gtgaattaga attagttaga cattatacat tattatcaaa taaaaacttt
    11701 ggagttgata caggttttta tcctttgggt tcttgtacta tgaaatataa tcctaaaata
    11761 aatgaagata tggcagcact tccaaacttt actggaatgc atccatacca atcttcggat
    11821 acagctcaag gttctctttc tttgatgtat gatttatcaa gaagacttgc agaaattaca
    11881 ggtatggatg aagttacact acaaccttca gcaggttctc atggtgagtt tacagggctt
    11941 atgattataa aagcatatca tgaaaataga ggtgaccata agagaaccaa agtgataatt
    12001 ccagattctg cacatggaac aaatccagca agtgcagcta tggcaaactt tgatgtgatt
    12061 caaatagcat ctgataaaaa tggagctgta gacataaatg cattaaaaga ggttttgaat
    12121 gatgaagtag cagctcttat gcttactaat ccaagtactc ttggtttatt tgaaaaaaat
    12181 ataaaagaaa tagctactct tgtacatgaa gcaggaggac ttttgtacta tgatggtgca
    12241 aacatgaacg ctattatggg gataacaaga cctggagata tggggtttga tgtggttcat
    12301 cttaatcttc ataagacttt ttcaactcct catggaggtg gaggtccagg agctggtcct
    12361 gttggagtta aaaaagaatt agtacctttt ttaccaatac cagtaattga aagaaaagaa
    12421 gataagtatg tactaaatta tgatagagaa aaatctattg ggaaaattaa aaatttctat
    12481 ggtaattttg gagttcttgt aagagcttac acatatatac taactatggg aagggatgga
    12541 cttaaagagg ctagtgaaat ggcagtttta aatgctaatt atataaagga aagtataaaa
    12601 gatgattata ttttgccaat agataccttg tgtaagcatg agtttgtatt aggtggattg
    12661 ccaagaggag aggccaatat aaaaactatc gatattgcaa aaagactact agattatgga
    12721 taccatcctc caacaatgta ctttcctctt attataaatg aggcacttat gatagaacct
    12781 acagaaagtg agagtataga gacattagat agttttatag aagctatgaa aagtgttgct
    12841 aaagaggcaa aggaagaacc agaactattg aaaactgctc cacataatac tttggtcaaa
    12901 agagtagatg atgcaagggc agtaaagaaa cctatactaa catggtcaca aagataaata
    12961 gttaaaaagt agagatttaa agtataaaga gaattaaatt tataagaaaa agctatctaa
    13021 cttaatctag tttagatagc tttttt
//
LOCUS       DAEMHW010000105        13042 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEMHW010000105 DAEMHW010000000
VERSION     DAEMHW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13042
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.105"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            118..999
                     /locus_tag="KPK67_002442"
     CDS             118..999
                     /locus_tag="KPK67_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2459397.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLRERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1207..2070)
                     /locus_tag="KPK67_002443"
     CDS             complement(1207..2070)
                     /locus_tag="KPK67_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBG2459398.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(2225..4159)
                     /locus_tag="KPK67_002444"
     CDS             complement(2225..4159)
                     /locus_tag="KPK67_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBG2459399.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGFDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4271..5284)
                     /gene="galE"
                     /locus_tag="KPK67_002445"
     CDS             complement(4271..5284)
                     /gene="galE"
                     /locus_tag="KPK67_002445"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBG2459400.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5308..6177)
                     /gene="galU"
                     /locus_tag="KPK67_002446"
     CDS             complement(5308..6177)
                     /gene="galU"
                     /locus_tag="KPK67_002446"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG2459401.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(6375..6695)
                     /locus_tag="KPK67_002447"
     CDS             complement(6375..6695)
                     /locus_tag="KPK67_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459402.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6685..8676)
                     /locus_tag="KPK67_002448"
     CDS             complement(6685..8676)
                     /locus_tag="KPK67_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459403.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8799..11342)
                     /locus_tag="KPK67_002449"
     CDS             complement(8799..11342)
                     /locus_tag="KPK67_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBG2459404.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESVSQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGVKEEIDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11620..11954)
                     /gene="rnpB"
                     /locus_tag="KPK67_002450"
     ncRNA           complement(11620..11954)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KPK67_002450"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12086..12763
                     /gene="srtB"
                     /locus_tag="KPK67_002451"
     CDS             12086..12763
                     /gene="srtB"
                     /locus_tag="KPK67_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBG2459405.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(12923..12997)
                     /locus_tag="KPK67_002452"
     tRNA            complement(12923..12997)
                     /locus_tag="KPK67_002452"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12963..12965),aa:Thr,seq:tgt)
BASE COUNT         4018 a         2287 c         1327 g         5410 t
ORIGIN      
        1 tttttaaaag cttttattct tactctataa tcatatataa taaaaatgta aaaatatctt
       61 aaaggttaac ttctacaaat atataaataa aaatacctat gaaaggatga acaaaaattg
      121 aatttatatc acttacgcta ttttgttaca ttggcacatt tagaacacta tacaaaagcg
      181 gcagaaaatt tatctataac acaaccaagc ttaagtcatg ctatttcttt actagaaaat
      241 gaacttgggg tggctctatt tgaaaaagaa ggtagaaata tagtccttac taagtatgga
      301 aaaatatttt taaaagatgt ggaaaaatca ttagaaattt tagattcaag tgtaaaatca
      361 ttaaaaataa ctggtactgg tgaaggacaa atagacttag cttttcttag aacacttggt
      421 actgatttta tcccagacat agtgcataaa tttttaaaat ctaatcctgc taaatcaata
      481 gattttaaat ttcatactgg tgtaactact gacattattc aaggtcttag agaaagaaaa
      541 tatgatattg cattttgttc gaaacttgaa aaagaaaagg gaattgaatt tatacctgtt
      601 gccaaacaag atttagtact aattgtacca tatagtcatc cactagctgc aaaggatact
      661 atagatttaa aagaaaccat accttatcct caaatagtat ttaatcagag aagcggtctt
      721 agatatataa ttgatgatat gtttaaaaaa ataaatcaac agcctaatat agtctatgaa
      781 gtagaggaag accaagtaat tgctggatta gttgcaaaaa atttcggaat tgctgtagtc
      841 ccaaatatga atatgcttag ctttacaaaa gtaaaagtaa ttcagattat tcacccaagt
      901 tgggaaagaa acttttactt agcatttata aaagatagat atttgcctcc tgcaattaaa
      961 aactttaaaa actttgtcat taaaaacgct caattataat ttattaataa aggggttatt
     1021 tttaaatatc ctcttttatt ctttttataa aataaaaaaa tctatacttt taaaagtata
     1081 gattcctaac tgacagtcgg catccgagtc tcagtaatta tcgttataca acagcaactg
     1141 gattcagttc ctgaataact aggttactta cattatatca gattttttaa aatttgtcaa
     1201 ttgtatctaa cgcatttttt caagccatgg tccaacaggc tctacaatag gaacttcttt
     1261 aaatccccaa tttcttatat cattcacact cgttggagta tatattttgt ttctgacaga
     1321 tttgaatatt ttgttttttt ctccatattg catacacttt ggattagctt ctattaaccc
     1381 tcttcttgct ctgctaagag gattacataa aatatctgct atttctaaac cttctctata
     1441 gtcagcatct tcttttttgg caattataaa acccttaacc ttatctctta attcttcatc
     1501 tatatctaag tatattgttc cattattaaa tacatcaaaa aacgcctttt gtacttttaa
     1561 gtttgagaca tcatcccttg actctataac tattcgtgca gattctacag tttcattatt
     1621 tacaaatgaa taaaaattct gaagaacatg tataaaagcc acaacatacg gttcctttga
     1681 aggtgcataa tatttcttta atctagcttt atctactgtt actgatatta tagtacactc
     1741 taatctagat agaaataaag gcaactcatc atagaataac ttaatctgtg aatcatcaaa
     1801 cttctcaaat gctcttgatt ttcttgatat atctgtcaaa tgtattccta tgttactggt
     1861 tccaaaacaa tactctttaa atgcatccat ttctttcctc aaagaacata cataatcgcc
     1921 ttgacttact gcatatttat attcaaaaat tactcctgtc aatgtaaatg ggtcttcatg
     1981 atttatatca gctcttccac tttcatcaat aaacattata tattttttct ttataatctt
     2041 actcaccctc ttaacatatg atgatagcat ctatattcat tttatatgag ttttaaaaat
     2101 attccaagat ttttatataa cttaaactta attttatact aaaaaattga gatgtaaaac
     2161 aatcaagctt ttattgattg aatttcacat ctcaattata attatttttc aatatatgtt
     2221 tttactattt tgcaagaata tccattaatt tatccattac tatataagat actccgcccc
     2281 ctatttgata aggagtttca acttttttat ttgcaaatac tctttgttgc tctgatgata
     2341 atcctgaatt atccattaaa actattggtg aatgtaattt tacagctaat ggtccagctg
     2401 ataaagcatc tactaaagca tctgatttac taactaatac agcctgtaaa tcagtatctc
     2461 catagaattt ttctattact ttggcattag tatctactct actatctcca tatattctat
     2521 ttcctgaaat atcttcagta gttatatcat tcatttttgc tattactgaa tcgttaattg
     2581 cacttggtcc acctataaag tatgcatttt ctaattttct ttctgttagc catttataag
     2641 attcttctgt aattccatct ttattgacta atattatagg ttgtttttct tccccagctt
     2701 tagaagctac agataaagca tctgcttccc catttccaga aaccatatat gcagttttta
     2761 tttcttctcg atttccaagt tcttcagcta ttcttgtagc agtttgatat ctatcttgac
     2821 ctgctattct ctttgtttct gtgttgaaag tagtttttat ttcttgctct aatttagaac
     2881 ttattgcatt ttctccacct atcaagatta ctgttttagg tgctaataac ttcatttctt
     2941 caacagtttc agttggtaaa ttagctgtat tagtaagtag tattgttgag tcatagctac
     3001 ttgctaaagg agttgctgtt ataccatcag ctattgcatt accatttact aatattacag
     3061 tatctgaact atcagcattc cagtttccaa ctgaacttat ctttttagct gtatcaaatc
     3121 tatttgcacc tataattgta tcccatgtag gatttatagt tccatcttca ttaaaagtat
     3181 aatctttccc atctattttt tgagaaccag ttagcatctt tccatctttg tctaaatagt
     3241 aagacttacc atctatttgt tgccatcctg tctgcattat tcctgttttg tcaaagtaat
     3301 acttattacc atctatttct tgccatcctg ttactgctaa accatcaaat cctagatagt
     3361 atttctttcc attcttttct tgccatcctg tttgcattac tccatctgag ttaaagtaat
     3421 aagtttttcc gtctattact ttccatccag atactcttgc tccatcagtt cctaaataat
     3481 attttttgtt attaattgtt ttccatcctg tttgcattac tcctgaagca tcaaagtaat
     3541 acatttttcc atttactttt agccaactat tcttttggta tgttccatca gattttacat
     3601 aatacttctt accattttct gttttccatc ctgtttgatt tagatttatc ttatattttg
     3661 ccgctatctt tacatagctg tcattactat aatctataat tacatctgaa cctacaggaa
     3721 cttggtcaaa taaccatcta acatctttat tgtgcattct tatacatcca ccagaaatat
     3781 gctttcctat agaactctca ttattatttc catgtattcc atatgtatct ccataagttc
     3841 cttttaatgc taatcccatc catctatctc ctaatggatt tcttggactt cctccaggta
     3901 tattaccttt ataatatggt ctatttttta tcttgttaac tacttttgtt ttccctgttg
     3961 gtgtttctga ccccttttta cctgttgcaa ctctaaattc ttttacaagt ttattattta
     4021 caaaatatcc tagagtattt tttcttgaat ttactataat cacatgttta ctggctgcgc
     4081 ttgattctgg tacgccacat gcaaatacta aaactaaagt cagtgctagt actaaatact
     4141 tttttagact ttttttcact ataacttccc cctcttttag aaacattact acatgtgtct
     4201 aattataaca taaaattacc aataccttat atttacaaat ttgttacatt tatacatata
     4261 agtatatatt ctattcttca tacccattag gattcatact ttgccatctc catgaatctt
     4321 cacacatctc ttcaagttca tacttagctt tccatccaag ctctttttct gcctttgaag
     4381 aatctgcata acacatggct acatctccag cccttcttcc tacaatctta tatggtattt
     4441 cttttccact tgctttagaa aatgctttta ctaaatcaag aacactatac ccctttccag
     4501 ttcctaaatt ataaacaaca agtcctggat tttcttctaa tttttgtaat gcttttacat
     4561 gaccagcagc taaatctaat acatgtatat agtctctaac tccagttcca tcatgtgttg
     4621 gatagtcatt accataaaca ctcaattcct tcaatttacc tactgctatt tttgttatat
     4681 aaggcattag attgctcgga actccatttg gttcttcacc tattctacca cttttatgag
     4741 caccgactgg attaaagtat cttaaaagag ctatatccaa tgacttatct gcttttgata
     4801 tatctactaa catctgctct atcataagtt tagttcttcc atatggattg gttacagaaa
     4861 gtgggaaatc ctctaatata ggacaagtat gtgggtctcc atatacagta gctgaagaac
     4921 taaatacaaa ctttttaaca ccatattctc tcattaattc aaataaattt aaggtactta
     4981 ttagattatt gctgtaatat tcaattggtt tttctactga ttcaccaact gcttttaaag
     5041 ctgcaaagtg tataattgat tcaatattat tttctttaaa tacaacatgc atttcatctt
     5101 tatttctgat atcaatatta taaaatttta ctggtttttt agataattcc ttgattctat
     5161 caaccactat tgagttgcta ttacttaagt tatcaactat aacaacttca taacctgatt
     5221 ctaaaagttc tattgctgta tggcttccta tatatcctgc tcctccagct actaaaactg
     5281 ccattgtaat tacctctttt ctaatattta tttttcactt actttttttc tcatatagtc
     5341 cattaaatca tcttttaaat tttctctctt tagagcaaag tctattgttg cctctaaaaa
     5401 accaatctta tctcccacat catatcttct accttcaaag ttataagcat aaatagcctc
     5461 ttgttttcca agagtttgaa gtgcatctgt cagttgtatc tctccacctt taccaggtgt
     5521 ctgtttctct aatatgctaa atattgcagg agttattata tatcttccaa gtatagctat
     5581 atcagatgga gcttcttcaa ttgcaggttt ttcaaccata tctttaactt tatatactct
     5641 atcttcgata tgttttacat ccaatatacc atatttatta gtatcttctt tagctatctt
     5701 ttgaacacct aatacagttg ttttatactc attataagta tctattaatt gttttaagca
     5761 cggaacatct gaatctacta tatcatcacc taataatacg gcaaaaggtt catcacctat
     5821 aaaacttttt gcacagtaaa ttgcatgtcc aagacctttt ggctcttttt gtcttatata
     5881 atgaatattt accatatttg atatgtctct taccatttct aacatctctg tcttaccttt
     5941 ttgctctagt tctaactcta attcaactga tttatcaaaa tgatcttcta tagatttttt
     6001 atttctacct gttattataa gaatttcttc aattcccgat tctatagctt cttctattat
     6061 atactgtaaa gttggtttat ctactatagg aagcatttct ttgggctgtg attttgttgc
     6121 tggtaaaaat ctagttccta gccctgctgc tggtattatt gcttttttaa ctgtcatctg
     6181 cattttttca cccttcttcg taaattttct attgtaccaa attaatatac catatttata
     6241 ctttttcttc aaactctata ttatcccaat atttaatgct ataagataat ataaggtaga
     6301 tgaaatttca tctaccttta gttttataag ttaatattgt attgtttata ttttatccaa
     6361 aacttataca ggtactaaat ttcatctaag atgcctttta tatcactatc tatatcttca
     6421 tctagataag aatctaaatc ttctaagtca acttctagtt cttgatatgt catatcaaca
     6481 ctgacatcct catctatatc catttctgat attaattctc ctaaaatact atttccatca
     6541 gaatctaaac cactatctat atcttcatct atatagtttt catttaaaat ttcatctaaa
     6601 tcaatctcgt aataaacttc attatttttt tcatcccctg agtccaatcc taagaactca
     6661 tagttaaaag tccaaacatt gtatttactt gacatacttt attcctcctg tgaaaaacat
     6721 attttcatct agtttattgg ttccataatc aacagagtta tatatttttt caattatgtc
     6781 ttttcttctt tcttttgtac tattatttaa tgtcaattgt ttaaatacat atgtatttgt
     6841 tattctctcg aataatattt cttttatact atcatcatca atatttctat agtttttcca
     6901 caataactca aataacttaa acatttccct actagtttca tctacataat aaggatacat
     6961 attttctact aaatggttta atcttgatat ggcttgtttt aaaaatacat caagacttgc
     7021 actttcactt tcttttaaca ttctataatt gtatttcttc ttatcttctc ctgaaattac
     7081 aaagatgata ttgtctaaac ttttttcaac ttcatttaaa taactgctta tattatatga
     7141 taagtcagac aatagatttt taatctgctt aaacacttgt acatcaagtt tagtattata
     7201 cttttcaaac aaatctattt cttgattttc taatattctt ggcatatatg ctgacaataa
     7261 atctaaattg tttctttgtt cttgtatttg cttataaagt gaatttataa cttctattct
     7321 tatttcatca tttaactctt tttctaaaat cttatcaaat tcagatttca tatagtaaat
     7381 atttccaaag aaattaaata ttatcatatc attcatagct ttagaccaat tcttatatat
     7441 cacttcaata gaattttctt taagtagagg ttgctttgca tatataaata catcttgcat
     7501 aagtttttga accatacttt tacttaagtt taaaagtact ttttgattat taaattttat
     7561 ttccgtttct acaaaattag aattttcgtt ttcaagttct ttattaatta caatgtcttt
     7621 tatatcacta ttttttatag atatgatttc tttttgaaaa gactttagaa tagtcaagct
     7681 atcgaaatct tcttgaataa aaaatggagc atgttttaat gtatatccca ctatttcatt
     7741 ttctatatct tttaggtcat ctatttgcat aatttctcta tttttatcat ttatatgtag
     7801 gttaaataca tttttattat catataatat tatatcttct cctaaattta acttagaaca
     7861 attatctttc gctgacttta caatagtctt cttagatttc ttatttataa aaattaattg
     7921 gtcttgatat tttactaaaa gcatgtttgt gtttttataa tcaaatccat ctatatttcc
     7981 tgatatcttg cagaatacta tttcacaatc tttaggaaga tttcctattt tttggttatc
     8041 tttaactctc tcatttaaat cagatggaac tacactatct atattgttat taaatataga
     8101 tacagacctt actataatcc cctctctttc catatgaaag tatcccttca atgacactct
     8161 tttatcttct aaaatgtatc tttctatatc atcaaaattt atttcaaatc tatttttccc
     8221 actgataaat ataaacttct cattatcttt attaattcca cccatataaa acttattgtc
     8281 atctaaactt aatatagtca tattatcctt tagatagaat ataaattctc tttgactttt
     8341 gcagattaaa tcatattcat ccaaagttat tttcatagtc aaatttgctg taacattttt
     8401 acttgaattt tcacttttta tgtaaaaagg caagttattt ccatagacaa ttatttgaga
     8461 atcttctaaa actgcctcta atttttcttt attgtctgaa aaactatata tatattttac
     8521 acctgtatcc tcattaactt catctgattt gctgattatt atattcccta gatttgttat
     8581 atgaagcttt ttatattctt ttgagtatct acttatgcca ctaagctcaa acccatctga
     8641 aagctcatat atgatttttt ctcctaattt caacatacct tcaccaccta aacagaatta
     8701 taagtatttt ataaaggtga ataaaatgtc tcaaaacaaa taatattttt tttaatactt
     8761 tttattctga gacatagttt ctcctttttt ataatgctct aagcaccttc aactatttct
     8821 tcataaagtt tatctaaatg tttaacatat tcacgatatc cagatttgtt ctttaactca
     8881 cttcttaaat cttcatattt ttgtttaagt tcttcagcac tcaaagaagg agctgattct
     8941 tttaattctt ctaacttata atcatagtac ttatcataca tagctatata tgtatcaata
     9001 gacttcttag ctagttcatt acatttttct tgtgcttctc catattgaga ataatcacct
     9061 atttttattt tatagtatcc attttcttga attattgttg aagaaatacc tttagcctca
     9121 tattcttttg ctgtttcttt tgctgttttt tcaactttaa gtgaacctgc ataaacagtg
     9181 taatatacac ctttaccatc tttatatgct tccatttctt ttgctttctt ttcagcttgt
     9241 gctttttctt gagcctcttt ttcagctttt gccttttctt ctgcttcttt tttctctttt
     9301 tcagcttgtg cttttttctc ttcttcagtt aatttattgt tagcttgttg ttgagttgat
     9361 gactttggaa tattctcttt atcatttttc ttattattgc ctactgttat aaaatatcct
     9421 acaccaacaa taaccactat aactactgct ataatagttt cttttaatct actcttctta
     9481 gaaacaacct cttcatcatc taaagaatct tctttatctg atacataatt tttcatttct
     9541 ttcttgtttt ctctatattt gtccttagaa gaatttttag attttttatt actattttct
     9601 ttctttttaa taggtattac tactgatttt tcattttcta atttttcttc ttttatttct
     9661 tttgcttttt tatcttctaa gtttttactt tctttttcaa gcttcgattc attatttgaa
     9721 gatacttttt catctaagtt attatttatt ggtttttcta tttttacact acgttcttct
     9781 tctatatgtt gacttggttc ttcttgtttt atttctttta attcattttg ctctatttct
     9841 tctgatttta cttgatgatt ttcaactggt ttatctaaaa ttatatcagg agaaatatca
     9901 tttaaattct cttcttcttg ttcaacttct tcattgtctt ttttaataaa tccaaataat
     9961 ttagccttct ttttactctt tttattttct ggttcaatta tatcttcttt attgctgtcg
    10021 tactctttat ctgtagtatc tttatcttct acaacgtcac ctatatcttg attattacta
    10081 tctatttctt ctttaacccc ttcttcagca tcatcttcta ttgaaatact acttctaaag
    10141 caaatatctt tatctgcatt ttttatcatg taaatgtatt cttcaatatc gatatcttta
    10201 tttcttctag ttctttttga tgcaatttca tgattttcta attcactata gttatctttt
    10261 attagattct gtgctttatt ccatgtatct tcactaattt ctgttttatt tttaattaca
    10321 tagtccacaa attccttaaa attttcagga ccaaattctc caattatagt cagtctcatt
    10381 gcaccttcta taaggtctct atttcttctc tttaaaaatt ctaatacaac tgttgcacaa
    10441 gcaaaatctt taataatttc tttgccaaaa ccatatcctt caacagaatg aagttcttct
    10501 aatccacata acttcttata ttcattgtgt attgtttgag atacactttc attatttaag
    10561 ctatttaact tatctataaa cttcgattct atatcatttg attttgattc atgttttact
    10621 tttgaatttt taatttcatt taatagcatg tcattttctg aattatcttt acctaattgc
    10681 aaatcctcaa tttcagttgc ttttatttct actttgtctt gttcgttatt ctcttctaca
    10741 ttattaggta ccacatcttt attgctagct aatattttgt ttacacagaa tataatttct
    10801 ctttttaatt ctcttttcgc atctgcttga tttttaagaa ataaatctcc acattctttc
    10861 ttttctaaac ttatttttct aagagatata ttaagaaggt ctttatatag gtttaaaacc
    10921 ttataatcct tttctaagcg tcttcttctc tctgatagtt ccattttatc ataagctaaa
    10981 aaactattta aatcattggc ttttagagag tctaatccct ctttcatagc cttatttaca
    11041 tactttctac attttgctgt agttaattct aaatcttttt ctaattcatc tatttcaggt
    11101 atgtacttat ctactatcca tttgccaata agtggttctg tatttcttgc taatgtctta
    11161 taaaacaata tattaaattt taaaagctct gcatattttg cttcattaaa ttctactttt
    11221 tcaaatttct tttccaaaac accatctagc ttttcattta ttttagactc ttgttttaaa
    11281 aaacttgctg tgacatttaa aacagtgttt cttattatat tattttcact caaaaccttc
    11341 actccttaaa tcaattattt tattgtaata tttacattgt gcaaagttgc taatttgatt
    11401 atgatatcta aataattatt tactaaattt gctacatctt atatcatatc atattattta
    11461 catcatttta ctttttcttt ttatatctat cttatattat accattttta atatctgtac
    11521 taacttatat tacaatattc ctcaaaattt taaaagtaga ttagacaaat ttacaaataa
    11581 aaaaacatca gtttaaactg atgtttttga ctatcgaaat aattaagcaa gtctataagc
    11641 ggagttctgt attagataat catctttcta ggcctattgt caccaatagg ctcaagcgac
    11701 ctacctaccg gagagtacga gcagcactct ttattcctat cttggtcttg ctccaggtgg
    11761 ggtttacata gccaactagt cgcctagctg ctggtgagct cttacctcgc ctttccatcc
    11821 ttaccacaat agtggcggtt tatttctgtt gcactatcct taggatcgct cccactaggc
    11881 gttacctagc accctgctct atggagctcc gactttcctc atgtaagatt acatgcgatt
    11941 atctggctta cttaatattt attttactta gtcatgttaa cacaaattct tttacatgtc
    12001 aaagtaatat tatactaaaa atttaaattt attcaaaaat agtgtaatat agatatataa
    12061 actacattta actcgaggtg atatattgaa aaaattatat agaatagtaa ttaacattat
    12121 actagtatta gtaatacttt acagtggatt taatatatac tcaaaactta ccaaatacaa
    12181 tcatgatact aaaatatcct cagaacttca aaaaaaagaa tataaaaaag aggatttatc
    12241 aaagattaac agtgatttta aattttggct ttctgttgag aatacaaata taaattatcc
    12301 agttgtacaa tctaaagata attcttacta tcttgacaaa gatttttata aaaaagattc
    12361 tatttctggt acactgttta tggattatag aaataaatct attgatgaca aaaatatcat
    12421 aatatatgga cataatatga aaaataaaac tatgttcaac aacctaaata agtttaaaga
    12481 tgctgacttc tttaaaaaaa ataataaaat aaaaataacc ttgaatggaa aagaattttt
    12541 atatgatgtt ttttctgctt atattgtaga atctgattat gattatctaa aaacaaattt
    12601 taataatgaa tctgattatc aaaattacat aaatgatatt acttctaaat cattgtataa
    12661 atctcctatt aaggtaaata gcaatgataa aatagttaca ctttctacat gtacctatga
    12721 atttgatgat gccagaatgg tgattcatgg tagattgatt taaaactcac agacacagtt
    12781 taaaacaata attttaaact gtgtcatctt ttatacctat aatttataaa ttagaccact
    12841 aaaagtattt aatacaaaat aacaaataca tcaaacaata aaaaacaata aaaaaagact
    12901 atgctaaaaa catagtcttt actggagctg gtgataggaa tcgaacctac aacctgctga
    12961 ttacaagtca gctgctctac cgttgagcca caccagcaca cctattttat tgtaaattag
    13021 taaaactaat ttttaaaagt ta
//
LOCUS       DAEMHW010000106        13017 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEMHW010000106 DAEMHW010000000
VERSION     DAEMHW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.106"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            158..1429
                     /gene="serS"
                     /locus_tag="KPK67_002453"
     CDS             158..1429
                     /gene="serS"
                     /locus_tag="KPK67_002453"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG2459406.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            1708..3213
                     /locus_tag="KPK67_002454"
     CDS             1708..3213
                     /locus_tag="KPK67_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG2459407.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            3327..4025
                     /locus_tag="KPK67_002455"
     CDS             3327..4025
                     /locus_tag="KPK67_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG2459408.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            4310..5398
                     /locus_tag="KPK67_002456"
     CDS             4310..5398
                     /locus_tag="KPK67_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459409.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            5636..6826
                     /locus_tag="KPK67_002457"
     CDS             5636..6826
                     /locus_tag="KPK67_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2459410.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            6917..8419
                     /locus_tag="KPK67_002458"
     CDS             6917..8419
                     /locus_tag="KPK67_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG2459411.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            8491..9747
                     /locus_tag="KPK67_002459"
     CDS             8491..9747
                     /locus_tag="KPK67_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG2459412.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            9919..12693
                     /locus_tag="KPK67_002460"
     CDS             9919..12693
                     /locus_tag="KPK67_002460"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG2459413.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     RMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMEEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(12758..12943)
                     /locus_tag="KPK67_002461"
     CDS             complement(12758..12943)
                     /locus_tag="KPK67_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG2459414.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
BASE COUNT         5028 a         1464 c         2354 g         4171 t
ORIGIN      
        1 atttgacaat agacaaaaaa gtgagtaaaa tataaataaa ataaaaataa caggttcgga
       61 taaagaaaaa ggtttcttaa cctcaagcat aaagtatatt atctaagtta tttgagatta
      121 agagataaat ccaatcttag gtactgagga ggaaaaaatg cttgatataa aaagaataag
      181 agaaaattta gattgtataa aaaaagcaat ggaaagaagg ggcgaaaaag actttaacct
      241 tgatgaagta gttaagttag atgatgaaag aagaaagatt cttcaagaag ttgaagttat
      301 gaaaaatgaa ttaaatacag catctaaaaa tataccaaat cttataaaag aaggtaaaga
      361 tgtagaaaat gaaaagatta aactaaaaga gttatctgat aaaattaagg taatagacca
      421 aaatcttaaa gaagtagaag ataagatgga atacctatta atgagaatac caaatgtacc
      481 acatccagaa gtaccacaag gtgaaactga tgaagataat gttgaagtaa gaacttgggg
      541 aaagactact acctttgatt ttgaatctaa ggctcattgg gaaattggaa cagaattagg
      601 tatacttgat tttgaaacag ctagtaaaat aactggttca agatttactc tttacaaagg
      661 attaggtgct agattagaga gagcattatt aaatttttac ttagacacga atacaaaagt
      721 aaatggatat acagaagtta ttccaccatt tatggcaaat agaaatagtt ttttaggtac
      781 aggacagtta cctaagtttg aagaagatat gtttaaaata gaaggtctgg actattttat
      841 gattccaaca tcagaagttc ctttaactaa tatacatgct aatgaaatac ttaaatttga
      901 acaattaccg ataaattata cagcttatac tccatgtttt aggtcagaag ctggttctgc
      961 tggaagagat acaagaggtt tagtaagaca gcatcagttt aacaaagtag aaatggttaa
     1021 aatagttgca ccagaagaat cttacaatga gttagaaaag cttactaaca atgcggaaac
     1081 tatgctacaa ttattgaatc tgccttatag agtagtaaag atatgtacag gtgatttagg
     1141 atttacggct tcttttaaat atgatgtaga agtttggatg ccaagttaca ataggtatgt
     1201 tgagatttca tcttgttcaa actgcgaaga tttccaagct agaagagcag gaataagatt
     1261 taaaagagat aaagattcta aagctgaata tgtacacaca ttaaatggct ctgggttagc
     1321 tattggaaga agtgttgcag ctatattaga aaattatcaa caagaagatg gttctgtggt
     1381 tgttcctgaa gttttaagac catatatggg agtttctgtt attaaataag agttaaatta
     1441 tgtattttta agcaatacaa taatatagag gtaataaaaa ataattaata taaaagtgat
     1501 acaataatat agaagtaata aaaaaatagt taatataaaa gtgatacaat aataatagaa
     1561 gtaataaaaa aaaataatta atataaaaat gtgtacaaac taatgaatat tgatatagta
     1621 agctatcata tgaaagagta tgtacacatt ttttttacta atttttcaca atatgaacat
     1681 aaataattta caggaaagga atcttttatg aaaattcaaa atactgagtg gggatatatt
     1741 gaatggaaac atacttatga tgaaaataat ccaaaacaag ctatgaatat atacattgca
     1801 gtaactatgc ctggtaagaa acattttaat catgttcatt atggtcaaga gcagatgata
     1861 tatattttag agggagaggg tttgtatatt ataaatggag tttggaagcc attttatcaa
     1921 ggtatgattt tttatataga atctggtagc acacatgaaa ctataaatac aggtgataga
     1981 gaaataaaag aacttattgt ttcaaataat gtagatgatg taggagagag tgaagttata
     2041 gatattaatc ctaataatta tttaaagaag accttgataa attatagtga aagtacatta
     2101 aatttgtatg cagcagtaga atcaattcgt ggtcaattta tagacccgtt taaaattcca
     2161 ttaattatat atgatgattc atggaatata gttttaaaaa atccttattt tccacttttt
     2221 tgttttgaaa aatgtaatcc tatgaaattt cctcaaaatt gtgattgtat gaatcaaaaa
     2281 agtagcaatc aatttgtatg tgaatatggg ataactatat ataatatacc aatactgtac
     2341 aaaagcaata gcataggtgt tataagagga gggtatgtac tattatctga tttgaattta
     2401 gatacagagc ataataattt atatgatatt ccagagggag ctgctagaag tataaaacgc
     2461 ctcttgaaac aaatatctaa aaatataata aatttttgct cttttaatga tataagaaag
     2521 gatttacaag aaaaagagaa gactatagca agaacatatc attatggaga gcaacttgaa
     2581 atgaatttaa aagtagctca agatatggtt actaatttaa gaataaatca tcacttttta
     2641 tttaatacgt taaattcaat ggcaagcata gcactagatg atggtagtta tgatttgtat
     2701 agtgcaataa tagatttatc aagaatgttt agatatacaa tgaggtctga tttaagattt
     2761 gtagaattag aatctgaaat attatacatt aaaaattatt tgaatttaca gaaattacgt
     2821 tatggagatg cacttaaagt aaaatatctt attccagaga agttatataa cctttctgtg
     2881 ccttttaact ttattcaacc tattgtagag aatgcattta cacatggatt tagagatatt
     2941 gacacagaaa agcgaattga aataattgca agattagatt ctcagtatgc tattatagag
     3001 attcataata atggaactat cttagatgga aataatatag acaaaataaa ggcaggtatt
     3061 cgtagtaata atggtcatgg tttgtcttta atatatacaa aattcacatc tgcttatgga
     3121 aataattttg atatggatat aaagtcgagt gataatgaag gaacttacat aatgattaaa
     3181 attcctattg aaaattataa ggagtatgta tagaacttgt gaaaaattaa taagttttat
     3241 aaatgttaag aaatatatta gatgttttta tgttgtttta tatatcttac ttttttatat
     3301 tattaagcta aaagggaggt attctcatga gaagagcact aataatagat gatgaagaag
     3361 ctgttctaaa aataataaca cattttatag aaatgaaaga tatgccaata aaaattgttg
     3421 gtaaagcaac atctggtgat gaagcagtag ataaaatcat tggtttagag ccagacatag
     3481 tttttataga tattcaaatg ccaatataca acgggttaga agtaatagaa aaaacatctc
     3541 atttatctaa aaaaataaac tttgtagtaa taactgcatt taactatttt gaatatgctc
     3601 aaaaagcttt gagattaaat gtaaaagata tactccttaa accattggat atgaaagaat
     3661 ttactaagtc tgtagaaaaa attattggat atcaatatac caataatgat ttattaaatg
     3721 agatactgga atatataaat ctaaactata gcaatgactt aaagttaaat gattgtgcta
     3781 gattattttt aaccaatcca tctaatatat ctaggatttt taaaagtaat ttaaatacaa
     3841 cttttatatc atatctaaat catataagaa tagaaaaatc tattgaatta cttgaaaata
     3901 ctacaaaaag tataaatgag attgcagaaa tagttggata taatagccta aataactttt
     3961 ataaaaactt taagatagaa aaaggaatga ctccaaaagt ttataaactg aacactaaaa
     4021 attgaaattg ataaataaaa agtcaatata gataattgtt tgagtgtgtc tgaataagta
     4081 tactttaatt atgataaaaa ttaaagcgct ttaaacatat ttaataatat taaaccttat
     4141 aaaagctgta cctatttata taaaaaaata tatataaata aaaagaaaaa gatatacaaa
     4201 ataaaaaaag tgaattaaat actgtttcaa aaagacagaa ttttcattta attcactgag
     4261 agtttatatt tcttaaagga aggtaacttt ataatggagg taatttaata tgtcagacaa
     4321 agtgaatatg aaaaatatta tctgtattgc aggcgcttat ctagcaacag caattggttc
     4381 tgggtttgct actggtcaag aaattctaca attttttgcc tcacaagggt atatgggttt
     4441 aattggagca ttaatatcaa tggtattgtt ttcatttctt ggtgctgaag ttataactag
     4501 aggtagagaa ttaaaactta aagaaccaac aagaatttac actttttatt gtgggaagat
     4561 tttaggaaaa ttttacgaat ggtttagtcc aatattttta tttgggattc ttatagtaat
     4621 gatttcaggt gcaggctcaa ctttaactga atactatggt ataaatcctt acttaggaag
     4681 agtattgatg gctgttgcta ctttaattac agtgtctatg ggtttagaaa aattagtaag
     4741 catacttgga cgtattggac cagtgatagc agtatttaca attgctgttg gattaataag
     4801 cttgtttaag cattttgatg gtttatctat ggttggagaa gctatgaaaa atattgaagt
     4861 tacaaaagca tcagaaagtc caatcgtttc tggtgtaata tataatacat taaatattat
     4921 tgtaatgatt gtttttttaa caggaatggg agcttctatt gaaagcaaaa aggatgcttt
     4981 ttggggaggt ttattaggtg gaatagtgtt tatgactgct ggtatagtta tgtatttggc
     5041 tatgatttca gatataggaa atttgtatat gaaagaaata ccatcacttt atttagcaga
     5101 taatatatct cctattgtag gtgtatgttt ttcagttgca ttaatattag gtatctatac
     5161 aacagctgtt ccacttttat ggtctgtaac caatagaatt gttgaagatg aacatcctaa
     5221 atttaaattg gtaacaacag taatagctat tttggcttgt ataggaggtt ttttaccgtt
     5281 tggtaaatta gtaaacattt tatatccata tacaggttat atggggcttt taattttagt
     5341 ctgtatggtg tataaagcaa ttacatgtag aaaaaaatca acacaaagtc aggaatagag
     5401 ccacttatta atagttttta tttaaagaaa ttttttatat aatccgaatt taaaaagtca
     5461 aaatagttaa ataaattgca aaacatgata atatatttaa ttaaaataat atagtaaaat
     5521 ataaatataa actaaaatta aatttataaa aaatatcttt ataaagatta ttaacttata
     5581 taatttgtag ttaaattata aaaaataaaa aataaaattt tggagaggtg ttagtatgca
     5641 tgaacttatt ttaatctcag aaaagtataa agaagatatt ttagattttt ttacagatat
     5701 tcatatgcac cccgaattaa gttttaaaga atttaggact acaaaagcta taaaagattt
     5761 attagtaagt ttagacatag aaatattaga tttaggaatg gaaacaggcg ttgttggatt
     5821 attaaaaggg aagtatgatg gtcccacagt agcactaaga ggagatattg atgcattgcc
     5881 aatatatgaa gaggttgata ttaagtataa atccagatat gatggtataa tgcatgcatg
     5941 tgggcatgat atacacacgt cttgccttgt aggatgtgca tatgtattgt ctcatattag
     6001 agatagtctt catggaaatg taaagtttat atttcaacca gccgaagaag tcaataaagg
     6061 tgcgaagatg ctagtggaac gaggagtaat ggaaaatcca aaagttgatg ctatatttgg
     6121 acttcataat catccagata taccttgtgg aaaaataggt gttaagttag gtgggttaat
     6181 ggcagcagta gacactatta agatagaagt aaatggattt ggaggtcacg gaggtatacc
     6241 caacagaaca atagacccta tagtagcatc aagtgcaatt ataatgggta ttcaaaccat
     6301 agtaagcaga aacataagtc cattagaatc agctgttata tcaataggta ctataaatgg
     6361 agggactgca aataatgtaa tttcagaaaa ggtagatatg acaggcacat gtagaagctt
     6421 tagtaatgaa gtacgaaaga aaatatcaga aaatttagaa aatatagttt gtgagatagc
     6481 tcgtggatat caagcaactg caaagctaga ttatttattt gatttaccag ctgttataaa
     6541 tagtaaagag atgtacgcaa tagcttgtaa gtctgtgtgt gatttgtatt cagaagatgc
     6601 tatagttgat ccaatacctt ctacaggagg agaagatttt tctatattca tggaaaaagc
     6661 cccaggattt ttttactggt taggggtagg caacaaagaa caagattgta tatatcagtg
     6721 gcataatcct aaatttaaag ctgataaaaa ttctatattg gtaggtacaa atgtactatg
     6781 tcagtctgtt ataaattata tggataaatt aaaaaataaa atttaagata aagcattctg
     6841 gagataaagt ttatgagggg gtattagttt gaggaaaatt aattcacagg aaaaaaacaa
     6901 agatagtgga ttattgatga gggcactaaa tgttgtagag agagcaggaa ataaacttcc
     6961 agacccagta accatatttt tattactatg tataattgtt gtaatattgt cagcagttat
     7021 atctaattta ggagtagaag aaatacatcc ttctacaaaa gaagttgtaa aggtagtaaa
     7081 tctactagag aaagagcaga ttcaatcata tttaggcagt atagttacaa acttccagtc
     7141 atttgctcct ttaggattag tactggttac aatgcttgga gcaggtgttg ctgagaaatc
     7201 tggatttatg gaagtgttaa tgaaaaaagg tataagtaaa gttccacaaa aacttgtaac
     7261 tgtagcaatc gtatttgcag gtatgttatc acatacagca gcagatgtag gatttataat
     7321 attaccacca cttgcagcac ttgtgttttt aggaattgga agacatcctc ttgtaggtat
     7381 gtttgcagct tttgcaggtg ttgcaggggg atttgcagca aatgttatgc ttagtactac
     7441 agatgtattg ttagctggat ttacaatacc agcagctcaa atgatggacc catcttacca
     7501 aggcaatcca gcaatgaatc tttattttgc ttttatatca gcgatagttt taacttttgc
     7561 aggagcattt gtaactgaaa aatttatagc tcctcgtttt acaaaatata atgattcaac
     7621 aaatgataag gatatacagg aattgagtga acttgaaaat aagggtataa aatacgcttt
     7681 attatcattg ttagttgtag tagtagtgat agttgcctta tgtataggtg acaatgcatt
     7741 tatgaaagat ccagagactg gttctatact atctagtaat gcacctttaa tgaaaggtat
     7801 agttcctata atcactatta tatttttaac tccaggatta gtgtatggaa aagtaagtaa
     7861 gaagataaaa agtgacaaag atttagtatc tatgatgggt agtagtatga gtgatatggg
     7921 tggatatata gtcctagcat ttatagcatc ccagtttatt aatttattta atcttagtaa
     7981 cttaggaaca attttatcaa taacaggagc aaaattacta gcagagtcgg ggatacctag
     8041 ctatggattg ataataggat ttatattgct atctggattt atcaatttat ttgtaggaag
     8101 tgcttctgca aaatgggcga ttttagcacc tatatttgtt cccatgttta tgctactaga
     8161 ctttaatcca gcattaactc aaatagctta tcgtattgga gatgcttcaa caaatccaat
     8221 aagtccactt tttccgtatt ttccagttat attggccttt gccagaagat atgataaaga
     8281 tataggtata ggtactgtca tatcgaatat gattccatac tcagtggtat ttacattaat
     8341 agaaataata atattgttac tattcatggg tattggcata ccattaggac ctggtggagg
     8401 aatatcttac gttttataga ccgttataat aaatttatca atgaagatta aactaataaa
     8461 atatattctt taattaagag gaggattatt atgttaaaaa caacaaaaat ttcagaccca
     8521 gagttataca agattgtagc tgatgagttg gttagacaag aacacaatat tgagatgata
     8581 gcttctgaaa gtacagcacc aacagaagta cttgaattaa gtggatgtgt atttacaaat
     8641 aaaacagaag aaggctatcc aggggctcgt tttcaagcag gttctgaaga agcagataaa
     8701 cttgaaaccc tagcaataaa acgtgcaaaa gaagtatttg gagcagaaca tgtaaacgta
     8761 caaccgtatt caggttctac agctaactat tgtgtatatt catcaatatt aaaaccaaat
     8821 gacactgttt taagtatgcg tcttgaccaa ggtggacact tgacacatgg tagcgcagtt
     8881 aattttttac atgacattta taaatatgaa ttttatgggg tagacccaaa tactggacgt
     8941 atagattatg atgctcttga agcaaaggca aaagaatgta gaccaaaact tataattgca
     9001 ggggcaagtt catatccaag attaatagat tatgaaagaa tatcaaaggt tgcaaaagaa
     9061 gtaggagctt actttatggt agatatggca catgttgcag gattagtcgc agcgaaagta
     9121 atacctagtc ctgtacctta tgcagatttt gtttcttcat ctacgacaaa gactttctgt
     9181 ggaccaagaa gtggtattgt actttgtaaa gcagaacatg caaagaaact cgataaaggt
     9241 gtttttccag gaacattagg gtctatacat cttaatacag ttgcagcaaa agccttttca
     9301 ctattatatt taagtacaga taaatttaaa aagataatgg agcaagttgt agtaaatgct
     9361 cagactctag cttctgaact gatttcacat ggttttagta ttgtaagtgg aggtactgat
     9421 aatcatatag taatggttga cttaagaagt aagaatctta caggaaaaca atttgaaaaa
     9481 gctcttgaat atgtaggaat aacagttaat aaaaatgtta tacctgacga cccacaatca
     9541 ccatttgtaa ctagtggtgt tcgtataggt cttacatcaa tatctcaaag aggtctgaaa
     9601 gaaaaagaag ttatacagat agcagggata atgaataagg tagcagaaaa tattgataat
     9661 aaagaagtat tagatgaatg taaagcagag gcacaagaac ttatatctaa gtttccatta
     9721 tatccagaag ggtattttga agattaaata ttttgatttg tatagctcac aatttataaa
     9781 gcctgtaaca taaaatttaa taatatataa ccactataag gtgttgatta atcaacacct
     9841 tatttttttt gaaaagtaat atacaagcta tcataagcat ataattcctt atagataatt
     9901 aacaaggggg ataaagttat gaggtattat aataaaccta ccaaagaagt attaaaatac
     9961 ttaaaaacaa atccagaaat tggacttgat gataatgagg tagaagaaag gaagcttaga
    10021 tatggattaa atgaatttac tataaaagaa ggtagaacat tttgggacga gttgggagaa
    10081 agtttgacag aacctatgat attaattctc ataggagcag cagtaataag ttcttttgtg
    10141 ggagaattac atgacgcatt aggtatttta ggggctattt ttattggaat ttctataggc
    10201 ataataactg aaggaaaatc taaaaaagca gctcatgccc tgtcgaaatt aacagaaaat
    10261 atagaagtaa aggtgcttag aaatgggaag ataattaaaa tatcaaaaaa tgatttagta
    10321 ccaggagata ttgtatatat agaaactggg gatatgatac cagcagatgg aagacttata
    10381 cagtctataa atttgaaact tagagaagat atgctaacag gagaatctga tgatgtagct
    10441 aagaatgctg atgctgtatt agatatggaa gtagtttatt ctaaaacaga aataatcgaa
    10501 caggatgcaa tacctgcaaa gcaagtcaat atggtatttg gagggactct tgtagcatat
    10561 ggtaggggca taatggttgt cactcatact ggtgataaga cagagatggg taagatagca
    10621 cagaatttat caaatgaaga ccagcaaact ccacttcaaa ttaaattagg gaagttgggt
    10681 gctaaaatag cgggaatatc aggcataata gctactttat tgtgtatgtt tatgattata
    10741 cagatgcaga gaaaaggtat gttaatttta gatacaagca gtgtattatc tttcttacaa
    10801 tcattagaac ctgctaaaaa tgcatatatg gtttgtattg cattaatagt tgcaactgtt
    10861 ccagaaggtt tgccaactat gataaatatt accttagcta taacaatgca aaagatggca
    10921 aagataaatg cattagtaac aaagaaagaa gcctgtgaaa ctataggttc tgtttctgta
    10981 atatgttccg acaaaacagg gactttaaca caaaacaaaa tgatggtaga ggtagcatat
    11041 gttgatggta aatatataag tggaggagag tatcaaagta atagctattt tgaacaaaac
    11101 tgtatagtaa attcaactgc agatatagaa aaagaagata attcatttaa atatatagga
    11161 agcgcaacag agtgtgcatt acttttatat cataatgata agaactataa tgaaatgaga
    11221 aaacaaacgt atttgatatc acaaatacca tttagttcag aagaaaagag gatgtcaaca
    11281 ttaattcgtc aagaggattc agatatatta ctttcaaaag gtgctccaga agtattattg
    11341 aaaaaatgtt cttatgtaca acaaggaaaa aatattgttc caataactcc aaaagtagaa
    11401 aaaagtattt tagatgagat taagaagtta caaattaaat ctatgagaac attaggattt
    11461 gcttataaaa aaatgagtaa ttctaaaaca gaagttgcaa tgacttcaga aggtgaattg
    11521 aatttaatag gaaattcaag aagttatatg gaggaagata atttagtttt tagtggattt
    11581 gtaggaatag tggacccatt gagagaaggg gtcaaagatt ccattgataa agcatttaat
    11641 gcaggagtag atgtaaaaat gcttacagga gataatataa atacagcaac agctattgga
    11701 aatgaattag gactattaaa tgatgggaaa aaggcagttg aagcaacata tatagatgta
    11761 ttgacagata aagaattgag agaagaaatc aaaggaatat ctattgttgc aagaagtaaa
    11821 ccagatacaa aaatgagaat agtttcagcg cttcaaaaaa gtggcgaagt tgtagctgta
    11881 acaggtgatg gtataaatga tgccccagca cttagtcaag cagatgttgg aattgcaatg
    11941 ggtatttcag gaacagaagt atctaaaaat gcagcagata taatattgac tgatgatagc
    12001 tttagtacta tagtagaggg aataaaatgg ggaagaggta tatatgagaa ctttcaaaga
    12061 tttatacagt ttcagcttac tgtaaatata gtagcattta taattgctat aatatctcaa
    12121 cttactggta aggatatgcc atttactaca atacaattat tatgggtaaa tataataatg
    12181 gatggtcctc cagccctagc attaggatta gaacctgtta gagattatgt attgaaaaga
    12241 aaaccaataa atagacattc tggaataata gctaggtcta tgtttgtaaa tattattata
    12301 aatgctattc ttataattac aattgtattt actcaatcag cttttaatat attgggagct
    12361 acatcagaag aacaaggtac tgttatattt tctttatttg cattcagtgc cctttttaat
    12421 gcattaaatt gtagagagtt tggtttaaat agtacaatac caaatttctt taaaaataaa
    12481 ctggcattgc aaataatagt agttacaggg ataattcaaa taatatttac acaagtgttc
    12541 cagagtttct ttaattctgt ttctttggac tttgatatgt ggataaagat aatactattt
    12601 gcatctacta tcctattatc aaatgaattt gtaaagctaa ttcttagaac aatgaaaaat
    12661 agtagaacta tgaactttaa tagtaaaaat taattcttat gaaaccgcaa agtactatat
    12721 attgtaactt tgcggttttt gtttgtttaa tgttgtttta tattaccatt ttcatattta
    12781 attttttatt aatctgaaca caatcttcca ttgtaattgc ttcatctgtg tcaacacatt
    12841 caaagtcaat atctaatttt tgtaatattt tgtatgcctc tttttgcaaa ggagtatcaa
    12901 atttaatagg tgattttgtg taaatatcac tgataaaaaa catgatttat ttcattcact
    12961 tccatttaaa atcttatagt tatagtgtat cataaggtta taaaatatag gtaaata
//
LOCUS       DAEMHW010000107        12836 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEMHW010000107 DAEMHW010000000
VERSION     DAEMHW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12836
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.107"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..540
                     /locus_tag="KPK67_002462"
     CDS             <1..540
                     /locus_tag="KPK67_002462"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2459415.1"
                     /translation="SMKNTGHIIKILRIEKKLTQDELAKNLNSSFNLKLNKGMISKWE
                     SNKAEPRFEYIKYISKYFDVSIDYLLGMTEYRNMYDNFDKKYDSKKLSLEVKEIEKNG
                     EKIKEKNKVLENKLLSSFNALNDIGQNEAIKRVDELTQIGKYVNKNHIDTIAAHNEHL
                     HEEGEIEKIYQDLDDMDNW"
     gene            558..1031
                     /locus_tag="KPK67_002463"
     CDS             558..1031
                     /locus_tag="KPK67_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBG2459416.1"
                     /translation="MNSYEKLLSEADDNNIIVREVSLISNSHGLYKNNRIALNKNTLN
                     NISEKACVLAEELGHHYTSYGNILDLNKVENSKQEYKARLMAYNKLIGLKGIIDSFNA
                     GCKTITEMAEYLDVTEKFLNEALECYKSKYGFSATLDNYVIFFEPRFSIMNANFL"
     gene            1096..2454
                     /locus_tag="KPK67_002464"
     CDS             1096..2454
                     /locus_tag="KPK67_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG2459417.1"
                     /translation="MLRVALYIRVSTEEQVLNGDSIRTQIEALERYSKENNFNIVDKY
                     IDEGFSATNLKRPNLQRLLRDVEKDKVDLVLMTKIDRLSRGVKNYYKIMETLEKHKCD
                     WKTILENYDSSTAAGRLHINIMLSVAENEAAQTSERIKFVFQDKLRRKEVISGTIPIG
                     YKIENKHLVIDKEKKYIVKAIFDEYEKSGSVRTLIETINNLHGELYSYNKIKNILRNE
                     LYIGIYNKRGFYVEDYCEPIISKKQFKQIQRILEKNKKTTPNKNIHYHIFSGLLKCKE
                     CGYTLKGNSSNVGEKLYLSYRCSTFYLNKNCIHNVTHNEKHIENYLLTNLKPQLHKHM
                     VKLEAQNEKIRQNKKSNKKDEKKKIMKKLDKIKDLYLEDLIDKETYRKDYEKLQSQLD
                     NITEEQESQIIDTSHIKKFLDIDINEMYSDLSRVERRRFWLSIIDYIEIDNNKNITIN
                     FI"
     gene            complement(2477..2593)
                     /locus_tag="KPK67_002465"
                     /pseudo
     CDS             complement(2477..2593)
                     /locus_tag="KPK67_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
     gene            complement(2689..3738)
                     /locus_tag="KPK67_002466"
                     /pseudo
     CDS             complement(2689..3738)
                     /locus_tag="KPK67_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
     gene            complement(4014..4682)
                     /locus_tag="KPK67_002467"
     CDS             complement(4014..4682)
                     /locus_tag="KPK67_002467"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBG2459418.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(4688..6061)
                     /locus_tag="KPK67_002468"
     CDS             complement(4688..6061)
                     /locus_tag="KPK67_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBG2459419.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(6058..7836)
                     /locus_tag="KPK67_002469"
     CDS             complement(6058..7836)
                     /locus_tag="KPK67_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBG2459420.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(7953..8276)
                     /locus_tag="KPK67_002470"
     CDS             complement(7953..8276)
                     /locus_tag="KPK67_002470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBG2459421.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(8263..9240)
                     /locus_tag="KPK67_002471"
     CDS             complement(8263..9240)
                     /locus_tag="KPK67_002471"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBG2459422.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(9255..9818)
                     /locus_tag="KPK67_002472"
     CDS             complement(9255..9818)
                     /locus_tag="KPK67_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBG2459423.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(9870..10361)
                     /locus_tag="KPK67_002473"
     CDS             complement(9870..10361)
                     /locus_tag="KPK67_002473"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBG2459424.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(10364..12289)
                     /locus_tag="KPK67_002474"
     CDS             complement(10364..12289)
                     /locus_tag="KPK67_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBG2459425.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTLP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVIFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(12291..12602)
                     /locus_tag="KPK67_002475"
     CDS             complement(12291..12602)
                     /locus_tag="KPK67_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459426.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
BASE COUNT         4175 a         2303 c         1728 g         4630 t
ORIGIN      
        1 tccatgaaaa atacaggtca tattataaag attttaagaa ttgaaaaaaa attaacgcaa
       61 gatgaactcg caaagaatct aaattctagt tttaatttaa aattaaataa aggtatgatt
      121 tcaaaatggg aatcaaacaa agcagaaccc agatttgaat atataaaata tatatcaaag
      181 tattttgatg tttcaataga ctatttatta ggaatgacag agtatagaaa tatgtatgat
      241 aactttgata agaaatatga ttctaaaaaa ttaagtttag aagttaaaga gattgaaaaa
      301 aacggagaaa agattaaaga aaaaaataaa gtattagaaa ataagctttt atcaagcttt
      361 aatgccttaa acgatattgg acaaaatgaa gcaataaaaa gagtagatga acttactcaa
      421 ataggtaaat atgtaaataa aaatcacata gatacaatag cagcacacaa tgaacattta
      481 catgaagaag gagaaattga aaaaatatat caagatttag atgatatgga taattggtaa
      541 aataggtagg tgaacttatg aatagttatg aaaagttact ctctgaagca gatgataata
      601 atattattgt tagagaagtt tctttaattt caaattctca tggattatat aaaaataata
      661 gaatagcttt aaataaaaat acacttaata atataagtga aaaagcttgt gtgttagcag
      721 aagaactagg acatcattat acttcatatg gaaatatatt agacctaaat aaagttgaaa
      781 atagcaaaca agaatataaa gctagattga tggcttacaa caagttaatt ggtcttaaag
      841 gtataataga tagttttaat gctggttgta agactataac cgaaatggca gaataccttg
      901 atgtaactga aaagtttcta aatgaagctt tagaatgtta taaaagtaaa tatggttttt
      961 cagctacact agataattat gtgatatttt ttgagccaag atttagtatt atgaatgcaa
     1021 attttttgta acaattcata gtatatttaa gagctgttca gctcctctta aatatacaat
     1081 aagggggaat gtaaaatgtt aagagtagcg ctttatatac gtgttagtac agaagagcaa
     1141 gttttaaatg gagatagcat aagaacacaa atagaggctt tagaaaggta ctctaaagag
     1201 aataatttta atatagttga taaatacatt gatgaaggtt tctctgcaac taatttaaaa
     1261 agacctaact tacaacgatt attaagagat gtagaaaaag ataaagtaga cctagtttta
     1321 atgactaaaa ttgatagact aagtcgtggc gttaaaaact attataaaat aatggaaacc
     1381 ttggaaaaac ataagtgtga ttggaaaacc attttagaaa actacgattc tagcactgct
     1441 gctggtagat tacatataaa cattatgcta tctgtagcag aaaatgaagc agctcaaaca
     1501 tctgaaagaa taaaatttgt ttttcaggat aaattgagac gtaaagaagt tataagtggt
     1561 acaattccca taggttacaa aatagaaaat aagcatctag ttattgataa agaaaaaaaa
     1621 tatatagtta aagctatatt tgacgaatac gagaaatcag gttctgttag gactttaata
     1681 gaaacaatta ataatcttca tggcgaatta tacagctata ataaaataaa gaatatacta
     1741 agaaatgaac tttatatagg aatctataat aaacgaggat tttatgttga agattattgt
     1801 gaacctatta tatcaaaaaa acaatttaaa caaatccaaa gaatattaga aaaaaataaa
     1861 aaaactactc ctaataaaaa catacattat catatttttt ctggtctttt aaaatgtaag
     1921 gagtgtggtt atacattaaa gggtaattct agtaatgttg gagaaaaact ttatttatct
     1981 tatagatgtt ctacattcta tctaaataaa aactgtatac ataatgtaac tcataatgaa
     2041 aaacatatag aaaactacct tttgactaac ttaaaaccac aattacataa acatatggtg
     2101 aaattagaag cacaaaatga aaaaatcaga caaaataaga aatctaacaa aaaagatgaa
     2161 aagaagaaaa ttatgaaaaa attagataaa atcaaagatt tatatttaga ggatttgatt
     2221 gataaagaga cttatagaaa agattatgaa aaattacaat ctcaactgga taatataact
     2281 gaagaacagg agagtcaaat tattgataca tcacatataa aaaaattctt agatattgat
     2341 attaatgaga tgtatagtga tttaagcagg gttgaacgca gacgtttttg gttatctata
     2401 atagattata tagaaataga taataacaaa aacatcacta ttaattttat ataattattt
     2461 ggactaacat atagtatcca ctaggtccga gtaaacatag aaattctcct tcttcaatat
     2521 ccaaactaat attatttaaa accctttttt ggtcataact tttaaacaca ttatttattt
     2581 ttaagtaact catatcttat ctctccttat atagttattt ttttatttta aattttatat
     2641 cctacttttt cttctaaaat tctttcatct aaattattaa tttaaacttt acttactttc
     2701 agattttgaa ccgtattttg attcccaagt tttaagtact gtacttctgt ttttggcagc
     2761 agagtttaaa tcaacttctt ttataagctg ttcaaatggt tatttagaat aaccttttgg
     2821 aactggatta tctgtcttta tggaagtaac tgctacttca tgtgcatatc tatccatagc
     2881 tgaatcactt attgcccaat ctaaaaatag ctttgcttct ttttttatat tatctttttt
     2941 aactaaggca tttgcttcta agtcccaacc tgaaccttcc tttggaaata caacctctat
     3001 aggttctcca ctagataatt gttgtgtagc tctatatcca agtgtaatac ctattggata
     3061 ctctccagag ccagctaact tcgcaggttt tgaacccgag tgtgtatatg tggctatatt
     3121 tttatgtaat ttatccatgt aattgtaagc atctttttca cccatcattt gaataagccc
     3181 tgatactgta agataccccg ttccagatga tgctggattt ggcattgtta taagtccttt
     3241 atattctggt tttattaaat cttcataact ttttggaata tctataccta gtttcttaag
     3301 ttccttataa ttaactacta ttgctgtctc tgggacactc attcctaccc aagacacatc
     3361 atcttcagta tctttaaatt ttttatcaac tttatcataa tttttaggct tatatgcttc
     3421 aagtgcacca tcttttttta gctctatcat gttcattgct gataatcccc atataatatc
     3481 tgcttgtgga ttatcttttt ctgctaatag ttttgaagct attacaccat gagaatccat
     3541 aactatctct acatctatat tgggatactc ttctctaaaa ccttccatat aataagctat
     3601 ctgctcttcc tctagtgctg catatattgt aagtttatta tttccttttc cttgagtagt
     3661 ttgtggtgtt gttgatatgc aacttgtcat aaatgtaatc actacaatgc acagacaagt
     3721 aaatataaat tctttcatat cttccccctt tgtgtttttt cattttttta tttgtttttt
     3781 attgtttttg cttatgttta tataatacac ctaatttgtt aatataaagt taagtgtaaa
     3841 ttaacagttc gttaattttt agaaattttt gttaaatata ttatttttag ttaaatacag
     3901 tatgttttta aacaatatat taatttttat agtatctaca ttttaattta aacataaaaa
     3961 ataagtctaa cctctataaa gagaatgtta gacttatttt tataagtatt taattataca
     4021 accaaatcat tttttttctt ttcttcaagt gcttttttaa gcaccatatc cttaaccttc
     4081 ataagtctag tagtacttgc tctctcattt tcttcaagct tcatagctat atacttaata
     4141 gtctcaatgt aatttggtat cattacattt tcaagagcat taacacgtct acgtgtcttt
     4201 tctatctctt gtgcaagaag ctgtgcactt ttttcacttt ctgcaagtct taaaagtggt
     4261 tgcatagcat ctgagaaagc ttccatagca gaatctaatt cacctgatgt aaaagcaaga
     4321 ccataaggat aaatatcact ttgattgttt tcagttttaa agtcaaacac aggaacatca
     4381 acactcatta tatttctcgt agaaacatct actgaaactg attgctttgg catcataagt
     4441 gctgaaccca gatactcctg actcataact gctcttgcga ttataaagtt tttataagca
     4501 gtatcaagag cattttctgc ttcttcacgc aatcgcttat tttctcttac aatctcaaga
     4561 aattgtttca taagttcatc aagtttatct ttaagaagct tatgacctct tgtagcagtt
     4621 ttaaggagtt tcttaagtct agtcatttcc atacgtgttg gatttatatt taatctagcc
     4681 acagatatca ctccttaggc atatattttt caaaatattc atcacggata cgtttaagtt
     4741 cactttttgg taacatacta agaagcttcc atccgatttc taatgtctgc tcaatagttc
     4801 tatcagttct aaatccttgt gaaacatatt ctttctcaaa ttcatctgca aacttagcat
     4861 acattaaatc aatctcagaa agtgcagatt caccaagtat agccataagt tcttttgcat
     4921 ccttacctct tgaataagct gcaaaaagct ggttcattgt atctgcatgg tcttcacgag
     4981 tcttaccttt accaatacct ttatctttta gacgagaaag tgatggtaaa acatctatag
     5041 gaggctgtat atctttttta taaagttcac ggctaagtat aatttgccct tctgtaatgt
     5101 atcctgtaag gtcaggaatt ggatgtgtct tatcatcctc aggcattgta agtattggaa
     5161 tcatagttat agacccttca tgccctttca tttttcctgc tctttcatac atagtagcaa
     5221 ggtcagtata aagatatcct ggatatccac gacgtcctgg aacttctttt ttagctgctg
     5281 aaacttcacg caatgcctct gcatagtttg taatatccgt tattataaca agtacatgca
     5341 tatcttgctc gaaagcaagg tattctgctg ctgtaagtgc cattctaggt gtagatacac
     5401 gctctacagc tgggtcattt gcaaggttta caaacactac agaacggtcg attgcaccag
     5461 ttgctttaaa atcgcttata aaaaattcag catcttcaaa agttatacca actgcagcaa
     5521 atacaacggc gaacttagaa tcagttccac gtacttttgc ctgtcttgca atctgaactg
     5581 caagattggc atgtggcaaa ccagaccctg agaatatagg aagtttttgc cctctaacca
     5641 atgtattaag tccatctata gcagagactc cagtttgtat aaactcggcc ggataatcac
     5701 gtgctgctgg atttattgga gctccattta tatcaagtct tttatctggt ataatttctg
     5761 gacctccatc aataggtcta cccattccac taaaaacacg acctaatata tctggtgaaa
     5821 ctccaaagtc aagactcttt cctaaaaaac gaactttact ttcagcaagg tttatacctg
     5881 ttgagctttc gaaaagctga acaagtgcag tatctccatt tacctctaga actttgcaac
     5941 ggcgaatttc tccatttgaa agttcaattt ctccaagttc atcaaatttt acaccttcta
     6001 cgccgtttat taacataaga ggaccggcta cctcttgtat tgacttatat tcctttatca
     6061 tagttcttca gcccccctct ttttaataag gtctgcaagc tcgttgtcta tttcatcttc
     6121 aattgaatcg taagctgcat ctatatcgcc ttcttctaca tacttagcac gaccaatctt
     6181 ttcaagaact gaaagcttga taatatcgtt tatagtaaca ttttgcttga tagcatcttg
     6241 tgctgactta tagaatttta atataagtcc catcattcta tattgtttat gaagtgatgt
     6301 ataagtatca acttcatgga aagagttttg atgaaggaag tcttctctta tagaacgagt
     6361 aacctctagt atcaatctat ctccatctga aagtgaatca acaccaacca attgaactat
     6421 ttcttgaagt tcagattcaa cttgtaaaag tttcatagct tgagctcttt gagctgagaa
     6481 attatcattt acatttttat ctgcccatac atcaactttt tcctgataga gtgaataact
     6541 agtcaaccag ttaattgctg ggaaatgacg tttatatgct aaagatgcat caagacccca
     6601 gaatactttt actatacgaa gcgttgcttg gctgacaggt tctgatgtat caccaccagg
     6661 aggagaaact gcaccaattg ctgtaagtgt tccttctctt tcatccatac ctagacaatt
     6721 tacctttcct gctctctcat agaattgagc aagacgtgaa cctaagtatg caggataacc
     6781 ttcttcacca ggcatctcct ctaaacgacc actcatctct ctaagagcct cagcccatct
     6841 tgatgtagag tctgccataa gtgcaacact atatcccata tctctaaaat attcagctat
     6901 tgtaatacca gtgtatatag aagcttcacg tgcagcaacc ggcatatctg atgtatttgc
     6961 tataagcaca gttctttgca taagtgactc gcctgttctt gggtctttta attcaggaaa
     7021 ttcaagaaga acatctgtca tttcatttcc acgctcgcca cagcctatat atacaactat
     7081 atctgcatct gcccatttag caagctgatg ctgtgtaaca gtttttccac ttccaaaagg
     7141 tcccggtata gctgctaccc caccttttgt gattgggaaa aatgtatcta taactctttg
     7201 acctgtaaca agaagtgaat ctggtgtttt cttttctcta tatggtcttt cttttctaac
     7261 tggccatttt tgcatcatag ttacaggtat atcattacct ttttcatctg taataacaca
     7321 aactgtatct tcaacagtaa attcaccaga atatatttct tttatagtac ccttaactcc
     7381 ataaggaacc ataattttac attctactac tgctgtttct tgaacgccac caattatatc
     7441 tcctgctaca accttatcac caacagattt tttaggttta aattcccact tagttgttct
     7501 atcaagtgaa gatacttcaa cacctttagt tatattagta cctgaaatat catacatttt
     7561 ttcaagtgga cgttggatac catcataaat agttgaaata agacctggtc caagttcaac
     7621 actcataggc attccaagag aaactacttc ttcccctggt cctagaccgg aagtttcctc
     7681 ataaacctgt atagaagctt tatctccatg catttctata atctcgccta taagatgttt
     7741 atctgaaact cttacgacgt caaacatatt cgcatctctc atgccctccg ctacaacaag
     7801 cggtcctgac acctttacaa ttgtgcctgt tttcatattc tctcacctgc aatcatatac
     7861 tttatcaaaa tttaaatatt ccctcctata ttttattagc acatatattt agcaccaata
     7921 aaatttgaga ttgtctttta ttcaactttc atctattctt taaataatat atctgcacct
     7981 atagcacgtt tactagattc tcttacttca ttcataccta atcccatact tcctccaata
     8041 cctggaataa ctataatggc aggaagttgg taatctttgt attttgctat tgattctttt
     8101 gcaagcaatg atgcctgttc tgtaatataa attatcgcat attcatcttc aaccaactta
     8161 tgtatacttt ttttaatttc gtcactatca taggctggga agatatctat tccaagagct
     8221 aaaaatccca tgatgctatc tttatctcca actactccta ccttatacat aagcatccct
     8281 tagcctttct tttatagtat ctgcgtctat attattaagc ttactagtca aaataattct
     8341 aattgttttt atctctgatt ctttagcata taaatatgca attagtggtt ctaatgtaag
     8401 aggcttaaat tttgctcctc taacatattc cataagatag tcatcacaaa gtttttcaaa
     8461 gacagtaaaa ccactatcca tgccattttt acagacatca gagtaagaag tagatttaaa
     8521 acatgatgct ggagtatctg aggaaaaagc ttctaagaat ttttccttat caagtgagcc
     8581 acctgaaaca aacacattca taaacatatc aaaatctttc ttcatatttt tgactcttat
     8641 aaaactcttt agatttgtta aatcacaaac tttcataaca taatctatta caaatttgtt
     8701 ttggctttct ttagcagttt ctttcatact ggtaaaggtt gctttatcta gaacaatatc
     8761 aaccatctgg ccattttgtg tcttattata aacatcaaat gcctctaata tagctgatga
     8821 cattatctta ggtaaatcac taaaactacg atttgctaaa gattctctaa gcttaataag
     8881 aggaattgta cctccatcaa ttaaatattt ctcaccatct acaccagaaa tttcttcttt
     8941 aatcagcact tttaaattgt gatagtcatt tttataaaga aatacatcaa caaatttttc
     9001 ttcaggagct agacttttaa gaatttcata tgtctttgat aattcttgag tcaaaatatt
     9061 ctcaaaatta tgtatattat tatttgatgt atcagcataa cctgcctcag ctataattct
     9121 taaagagtct tcagggcttt tggcttctgc catttgaata aacatttgtc tgttaagaag
     9181 ctgtttttca agcactctta ttcgtgcaac ggcataggga taagtttttt cttctgccat
     9241 tttttaaccc ctccttaatt aaacaaaatt tctgctgcaa tttgttcaat atattcatgc
     9301 tctactgcaa ttatggcttc aaatgaataa ttgtactcaa tgtcaccttt tttaactatg
     9361 aaacctccag taatatctct agtttcatca gacactttaa tgcctttttt tgatagcaca
     9421 tcttttaatg tttttttatc ttttttagat aatactatct ctgaatcatc tgtacaattt
     9481 gatttttcaa tcatgtttag tataatctct tcatattcat tatctttaag atttaaaagt
     9541 ttgtttttag cttctaaaat tacctcttct aaaatctctt gtttttttga taatatttgc
     9601 tttttagcct ccatataagc tcctgatatt tccttagaag ccgctttttc tgcttctgcc
     9661 tctgcaagct ttgtatagga agccatttcc ttttcagctt tttcattagc tgaatttact
     9721 tttaaatctg cttcactttt agctttatcc aatatttctt gtgcttcttg tttggcatca
     9781 gctataattc tgtcaatcat tttttgttca ttacccatag gagtgcttcc tccttttcat
     9841 tttttttact tatgaattat aatttataat taaccaattg caattccatt gtaagctaag
     9901 aaagaaacaa gtagtcctaa aagagcatat gtttcaacta ttgctgcaaa tgttatagct
     9961 ttaccaagtt cttcaggtct ttttgcaact attccaactc ctgctgctgc tgctttacct
    10021 tgtgctatac ctgatactaa accaactaaa ccaattggaa gtcctgccat aagaagttgt
    10081 aaaccttgag ctgatgttgg tattgcatct ccaccaataa tccctgcttt tgataaaata
    10141 aggaaagcta tgataaatcc atacagacct tgtgtaccag gaagaagttg taaaactaga
    10201 agttgaccga atttactagg gtcttctgtt acaacacctg ctgcggcttg acctgctatt
    10261 gaagtacctt tagcagaacc catacctgca aataatgctg ctatagcagc tcctgatatt
    10321 gccaaaaatt gaccatttcc taacgcgttt aaaaattcca tatttacatc tcctttttat
    10381 taatgcttgt atattttgtt ttatacttaa acggaacaaa aggaactcct ccgccttcat
    10441 aaaacttatt gaaaaattct acatactgta acctacttgt atgaacatat gcacctagtg
    10501 catttataag aaggtttatt gtgtgtccta caacaccaat tacaactgca agtatcggac
    10561 cagctatagc tcctaaaagg ttgataactt gtgctataac tcctgttgtt aaacaaagcg
    10621 ccataattct tgtataagat aatatatcag caaaataact tgttatacca tagagacttg
    10681 aaaatccttt aaaaagctta acaggaatac ctttaaagct tcttccttga gtaagtacaa
    10741 gacctatagc acctataatt gctaaatatt ttcctacttc tgaaaataca cctatgtccc
    10801 ctgcaacaaa aggtattata agtagacata cacctgttat acacaaatac caaaaaccta
    10861 tgtcaaagat tgcatctatt acctttccac ttcttattaa tgtatatgct tttattccca
    10921 aacctacata tatatggata ataccgaaca gtaacgacat tcccataagt gtcataacat
    10981 cctctaaagg attaattaat gcttttatag gaattaaatc ccccaaaata cccccgaata
    11041 taagacccca tacagtagtt gaaactccac agataccaat aagtttaatt aaatttcctt
    11101 cacctttttg tatctttaat ttatatacaa caaatccaca aactactgca attattaacc
    11161 catatgcggc gtcacttagc atcattccaa agaatataac aaagaagaaa gttaaaattg
    11221 tatttgggtc aatatctttt gtacttggat agctgtacat gttagttaca ctttcaaaag
    11281 gtgttactaa tttattgttt tcaagcagaa ttgggaagcc ttcttcttta cttccttctt
    11341 cagtctgaac acaacaatca aacttttctg taatttcttt aattaattca tctgcccttt
    11401 tgcttggtaa ccaaccatta agacagaatg ttgtttttgt tttgacaagt ctctctataa
    11461 tctttttttg gtctctttct atagtgtaaa aatcatatag gttttcaagc atgtgtatac
    11521 tatctgcatg tcttttaatt tcctctgttt tgtccttaga aacgttttcc tgtctaatta
    11581 tcatttcatt gtatttcttg atagcttgta acggtgtacc ttcttcattt ggtaatgaaa
    11641 ccactgaaaa attaaattct tttaaaactt ctaaaacatc atcaaaagtt tcttttaaag
    11701 taattaaata aacatagttc attaacttgt cagaattgac aatcccaata acactttctg
    11761 gtatttcatc ttctaatttt actcttaaag catcaatgtt tgcgcttatg ggcaaagttc
    11821 caagtatagt ttttgtatac ttagtttcca tattttcaag aggaatatca aagttcaccc
    11881 atggttctaa catactttta ttgttgttaa gtaagtttat gttagacctt attgtaccta
    11941 tctccttgtg caaattgttt aactccagag cttcagcata aacttgccct tctttttcac
    12001 tatcaatttt gttaaactct ctttttggtg caagtaggga ttttttttgt ttcactagtg
    12061 aagaaacaat attaattgct tggtttacat gagacatttt ctgctctagc attataactt
    12121 cagattcttc actatctttt acaagataat tttttaattc ctcctcctca gtaagaaagg
    12181 cactatcatc aatctgaaca aacccacggt tcataagtag ctttaaaatc tggctttttt
    12241 gagcttctaa gccaacaata gatattttgt tcatattaac tattgccata ttatctcact
    12301 acctttccaa taaccgcttc aatagcctta ctttttcttt cttcagcagt tttttcaatt
    12361 ttcagacatt cacttttagc attagctaag attttatcaa cctctgctct actgtcggtg
    12421 actgccttct caataagagt ttcttccata agctttgctt gtttttttac atcagcaaat
    12481 atgctttcac atttgtttac agcatcagct ctgatagact ctgcatcagt aacagctttt
    12541 actttgatac tgtctgctgt ctgctctgcc tcaacaatgc tttttattat ttcaaaagcc
    12601 atccaaatca cctctcttac ctttttacta tgtatataac caattagatt tttttattct
    12661 aattagatta ctacctcatt gttaaagtat aaacagcatt tttatataat aattaatatt
    12721 tttcaacgtt tatttcttaa taaaacatat tttagaatag tacttgccta gatttacatc
    12781 cttttttgac ttacttttag actatgacat gagtatggtt tttaaatacc attatt
//
LOCUS       DAEMHW010000108        12811 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEMHW010000108 DAEMHW010000000
VERSION     DAEMHW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.108"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            111..299
                     /locus_tag="KPK67_002476"
     CDS             111..299
                     /locus_tag="KPK67_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBG2459427.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(459..611)
                     /locus_tag="KPK67_002477"
     CDS             complement(459..611)
                     /locus_tag="KPK67_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBG2459428.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(709..1185)
                     /locus_tag="KPK67_002478"
     CDS             complement(709..1185)
                     /locus_tag="KPK67_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG2459429.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1404..2165
                     /locus_tag="KPK67_002479"
     CDS             1404..2165
                     /locus_tag="KPK67_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2459430.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRYAIVIYTRDK"
     gene            complement(2425..4353)
                     /locus_tag="KPK67_002480"
     CDS             complement(2425..4353)
                     /locus_tag="KPK67_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2459431.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNNSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            4587..5039
                     /locus_tag="KPK67_002481"
     CDS             4587..5039
                     /locus_tag="KPK67_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2459432.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            5042..5350
                     /locus_tag="KPK67_002482"
     CDS             5042..5350
                     /locus_tag="KPK67_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG2459433.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5464..6570
                     /locus_tag="KPK67_002483"
     CDS             5464..6570
                     /locus_tag="KPK67_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG2459434.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITESAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            6645..9323
                     /gene="mngB"
                     /locus_tag="KPK67_002484"
     CDS             6645..9323
                     /gene="mngB"
                     /locus_tag="KPK67_002484"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG2459435.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSSQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIKTSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MIVKYSLTIPTSLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEYSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9640..11973)
                     /locus_tag="KPK67_002485"
     CDS             complement(9640..11973)
                     /locus_tag="KPK67_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2459436.1"
                     /translation="MDTKLKKFKDKYIVTIIIFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKAVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(11948..12664)
                     /locus_tag="KPK67_002486"
     CDS             complement(11948..12664)
                     /locus_tag="KPK67_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2459437.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         4782 a         1788 c         1663 g         4578 t
ORIGIN      
        1 caatatgtgt aaggcaaata tatatttcta ttgcaaattt ttgttattta tgataatata
       61 aatatgtttt tagtctttaa atagttatca ttttaaggag gtgtgcgtag atggcaaaag
      121 tatgtagcgt atgtggtaaa ggtaaggttt ctggaaacca agtatcacac tcaaataaac
      181 ataataaaag aacatggtca gctaacttaa gaagcgtaag agcgattata gatggagctc
      241 ctaagagagt aaaagtttgt actagatgtt tacgttctgg taaaattgaa agagcttagt
      301 aaccacgttt aaaagcctac ttccgtaagt aggcttttta atttaatcat ttttactctt
      361 tctcataaat tttaggatta tacttgaaat ccattttggt aattttattg ttactacttt
      421 catcaatact caccccacac tttatagtta aaactatttt aacaaaaaaa taataatgct
      481 ccacatccaa taagtattaa tgcggtaagt gctgaccaaa tccagccagg aagcaacatt
      541 gacagaatta cagccattcc tgttgctaga caaagtattc ctagcaatac cttagacttg
      601 ttatttctca tggaacacct ccacatagaa cctgtatcta taaatatttt catattcaag
      661 tttatgcgac agttttccat tttagaccaa taaacacatt atgtaatttt aatttttcat
      721 actagaatta ttactgaact ccgatataga ttctacaata tccaaaattc ctttttttct
      781 acttaacctt tcaggtccac tatataaaac aactggtata actttaggaa gtttaaaatt
      841 tttatcttct atttcttcaa ggcttttttt cataataaaa cttttccaaa tttgttctct
      901 atacgatttc aatcttggga caatttcagt atccatttct gtttgaaatt ctaacactat
      961 ataatgtgat actttgccac ttttagaaaa taccttataa attatatcaa cttcttttcc
     1021 tttatattca catataaaag atgtttctaa ctctatattt ttaagagtta attcttttgc
     1081 tattgagaca cttgtaaaat tttgaatcaa acttaaaaaa ctttctttat ctgaatacat
     1141 tcttctgtac tcttctcgtt tttcattgct tctttttaaa ttcatacagt tcctcctaga
     1201 ctatagaccc tctatttttt agtaaagata gtctataaat tttatttttt aattttcatt
     1261 agtgagtatt gacagttgtg tgtaaaatat ccagtaaaga tattttttta tatttttatt
     1321 aaaagatata aatttttata ttttaatgta ttgacattta cgttgcgtaa aagtgtatat
     1381 ttaatttata ggaggtacta cttatggaat atacagttca aaaattaagt aaactagctg
     1441 gtataagtac tagaacactt agatattatg atgaaatagg tcttcttaaa ccacttaaaa
     1501 taaattcttc aggttatcgt atatatggtc aaaatgaagt taataaactt caacaaatac
     1561 ttttttatag agaattagga attaacttag aaaatataaa aaacattata aattcaccaa
     1621 acttcgatag tttaagtgcg ttaaaagaac accataataa attactagcc aaaagaaagc
     1681 aaattgattt actaatagaa aatgtaacta aaactatagc tctgaaggag ggaaaataca
     1741 ctatgacaga tatagaaaaa tttgaaggtt ttaaagaaaa gatgattgat gaaaatgaaa
     1801 aaaattatgg aactgaaatt agagagaaat atggtaaaga tattatagat acatcaaata
     1861 aaaaacttaa aaatatgtct aagcaagatt atgaaaattg gcaaaaccta aatatcgaaa
     1921 taattagtaa acttaaaaaa gcttttaaaa ctggtgatgc ctctagtgaa ctggctcagg
     1981 aagttgcaaa attacatcat aaatggttga gttacacttg gaacacatac tctaaagaag
     2041 ctcattcctc tctagcacag atgtatgtat atgatgaacg ttttacatca tattatgata
     2101 gagaacaacc tggattagct aagtttttaa ggtatgctat agtaatatat acaagagata
     2161 aataatataa aataataaag gtggtgtctc aaaatgaact ttttagttca tgagacactc
     2221 ttttttgtat gaaatcaaat atatttttat cattttatca atgaaaaaca ggtttctctc
     2281 tctattttga gacagaatct ttattgtata ataattataa tcaatacaaa ataataacaa
     2341 attatataaa acttacttat actatttatg ctaaaaacaa aaaatatttt attaaagttt
     2401 ttaaaactta tgtaatataa aaatctatac tatagacttg attatggaaa gaaaattatc
     2461 aaaatcatta caattagata tactatctct aatatcattg tcatgaatta tctttaaaag
     2521 gatatcataa atggagttta tttttatata atcatcttta tttatagcca acatcatgat
     2581 taatttaaca gtagaatctt cactccaata tattggttca ttcaatatgg aaatacaaat
     2641 cttagttctt atagagctta attccatagg atgaggtata gctaaaaaat catctatatt
     2701 ggtagaagat aaactttctc tgtaaaatac agattcagca aatgatggaa atacaatttc
     2761 actttgagtt aatttgttgc acatcaactt tataagctca tctttatctt tgattttagg
     2821 attaacaata aataaatttt tatcaaagaa attgtccaaa agattactat aaatatggga
     2881 attattagtg attgacttag atatattctc tatatctttt tttaatagtt ttaaatccac
     2941 taatacaata ggaatactat catgatttaa aggaattgtc gatattatta tatctacatc
     3001 tgacaattca ctagctaaaa attgattaaa tgatagacat tgaagtatat ttattttatc
     3061 atgaaaaact ttgtttaatt gaacctctaa taacctactt gaaccatacc ctgaaccaca
     3121 tactaatact acgtttttac acttaatatt actttgaaaa aatctctcta ttccagcacc
     3181 tatatgcagg gatacatatc caatttcatc ttctgttaaa gagtaaatac tatttttaaa
     3241 aactttttct atagcattta aagttatttc aaatgctaaa ggataattac tctttattgt
     3301 atttataagg ggattcacct tatttaaatt ataagattta gaattgagaa ttgatttaaa
     3361 gtgcagtact aaatcatcaa ataatacact attatttcta aggtcaaaag tatattgatt
     3421 gttgattact tctaaaattt cttcaacata ctctataatt ttagtatcta ccctattgga
     3481 aacgttatcc aaaagagaac ttttagttat aaaatgatta tatagataaa ctctgtctgc
     3541 ctttgaaata attatagtat atttactctc aatataatta aatatatttt caacagttgt
     3601 attcgattga aaatctgtta aaacaacatc taaaacatta tctaagcagt aaccatcaag
     3661 tattctactt acagtaatag ctaaatggat aataaagttt tttagattaa aatctaggaa
     3721 attgatatta aatttattaa tttcacttaa cactaccctt tgaagctcta ttaaatctac
     3781 attattaaat aattctattt ctctatctgt aattccaatt atatagtttt caaaattcct
     3841 atttatcata ttattagaaa tacatcttcg aatatctatt tccttacctt caactttaac
     3901 tccataatat ggcttagaaa ctaatattaa attatacttt gacaaaatag gctttattct
     3961 ttttatgtca tttagtattg taaatttact cacatacaag ctattagcta aatcttctag
     4021 ttttatatat gtattgttat agagaatata ttttaaaata tattgatttc tttctatagg
     4081 agaatctggt atttcacttg aatccataat atcatcagat attaatgcta gatactgttg
     4141 gtataatgct aaattaagaa tatctatata atatccttct ccttttttta ttttaataat
     4201 agcaccattt ttctccaaag ttccatttat agaagtaata tcatttctta tagttctctc
     4261 actcactttt aaatgattac ttaaagtttt acaagtaatt ttatcatcac tttcatttaa
     4321 taatttaaac aacgtgagca atctcttgtg cataaatatc cccccttata gaatttcttc
     4381 taaattatac tatttacact catataatac aattactcta ataaaattgt attataataa
     4441 tttatttcca cttctatatg gaaattagtt attatcataa tataaatttc catgtcgaag
     4501 tggaaattag ttatatttaa tttaattagg ataatgatta caatataaat ataagttaag
     4561 acattatgaa tggagtggaa aaatatatgg tactacaaga aaaatatata tttatagata
     4621 aagacttaaa aagcaaagaa gaagtattaa attttattgc agatagtgct tatatactag
     4681 gtattacaac taataaagag gctcttttaa aagaccttct aaaaagagaa aatgaatttc
     4741 caacaggaat aaaagatgga tttgcgatac ctcatacaaa aagtgtaaat gtaaaggaag
     4801 cttccatact ctatattaaa acaactaacg agttagagtg gaaaacttta gatgatagta
     4861 aggttagata tgtatttagt ttgttagccc cacataaaaa tgaaggaaat atacatttga
     4921 aaactctatc taaattagcc acatgtttaa tggaggatga ttttacaagt gaagttaaaa
     4981 atgctagtga tgcaagtcat ttagtaaaat atataactaa taaaatgaag gaggaataat
     5041 tatgaaaata gtagcaataa catcatgtat agcaggatta gcacacacac ctatggcagc
     5101 aaaagcttta gaacaagctg catcaaaaat gggtcatgaa atcaaagtag aacaacaagg
     5161 tgctatggga cgagtaaata ctataacaaa agaagaagca caagatgcgg actttatact
     5221 tatagcatct gaccaaacta tatctggaat ggaaagattt gatggtaaaa aagtgataaa
     5281 agtcaaaata ggtatagcgt taaaaaaacc agaagctgtc ttaacaaagt gtatagaagc
     5341 tatttcttaa taaaaaaatc tcactctaat atagaatgag aaaaatcaaa aattaaaaaa
     5401 ttgtctaata taattataac atattatcaa aaaatataac aaacttatat aggaggatta
     5461 aaaatgagta aaaaaaataa atctctttta agtgaactta agggacactt aatgacaggt
     5521 atctcatata tgttgccact tgtaataggc tcatctttag ttgtagctat cccaaagctt
     5581 acagctttag caatgggtat aacaagttta gatgcttata aaaatgtatc tggtttttat
     5641 catattcttt acttaatgga acaagttggt tggactggca taggtctatt aaatacggta
     5701 ttagcaggat ttatagcatt ttcaataggt gagaaaccag ctatgggtgc aggttttatt
     5761 ggtgggctca tagcaagtaa tactaatgca ggattcttag gagcagtaat agcaggtttc
     5821 tttgctgggt atgtttgtaa gtttttaaag aaaaaaatta aaatatctgg gtcagcatca
     5881 ggaatgatgc cacttataat aatgccactt ataacagttg gattaactgg ttttttaatg
     5941 agtgttgtat tagcaggacc tttaggaaac ataaatactt cattaaatgc ttgggtagct
     6001 gaaatgtgtc aaaatggaac aaattcggtg gtattggctt taattttagg tgcaatgata
     6061 ggattcgatt taggtggacc agtaaataaa gcagcttgga tggcaggaaa tgcattattg
     6121 ttagagggaa tctatctacc agcgatattg gtaaactgcg caatagttat acctccacta
     6181 ggatatggtt tagctacatt cataagaaaa tcaaaatttt ccaatgcatt agctgaaaca
     6241 ggaaaaggca atatagttat gggcataatt ggaataacag aaagtgcaat tcctttcact
     6301 ttaacaaatc cattaaaatt agttcctgtt aatatgatag gatgcgcttt gggtgtagga
     6361 ttatcagctt tgttaggtgt acatgctata atgcctccag ttggtggatt atatggattt
     6421 atatcaatag gttctggatg ggcttactta gtaggtgcaa tatttggagc tttagtaata
     6481 gcaatattat caacaacttt agtagacttc aatgaagatg aaactacgga aaatacagaa
     6541 tttgatgaaa tagagttaga tataatgtaa attaaaaact taattttaga taattgagaa
     6601 ttgagtgtac tctcaattct caattaaatt ttgggaggat ttttatgaaa aagacaaaag
     6661 tacatgtagt tccacacagt cattgggata gagaatggta ttttacaaca tcaaggtcaa
     6721 aaatatattt aatgaaagat tttcaagata tattagacat attggaagaa aaagaagact
     6781 ttaaatactt tactatggat gcacaagctt ctctcttaga tgattattta aaatggagac
     6841 ctcaggatga atatagaata agagatttag ttcaaagaag aagattagtt attggacctt
     6901 ggtatactca aacagaccag ttggttatat caggagaatc tataattaga aacctttact
     6961 atggaataga tagatgcaat gaatttggtg aacctatgag agttggatat gtaccagatt
     7021 cttttgggca atcatctcaa atgccacaaa tatacagagg ttttggaata gatagttcat
     7081 tattttggcg tggagtttct gatgatatgg tagatacaac agagtttata tggaaaggtt
     7141 ctgatggttc aaaagtatta gcagttcaaa taccatttgg ttattactat ggaggtaata
     7201 taccagaaga tgattcagat ttaaaagaat atttaaaaga tataattgga aaacttaagg
     7261 ttaaagcttc tactaataac gtatattttc caaatggatt tgaccaagca ccaataagaa
     7321 aaaaccttcc tgatatatta aaaaaagcta atgaaataga taatgaaaat gaatacgaaa
     7381 taagtgatat agaaaactat atagattcag taagaaatga aagaaatgaa tttacagagc
     7441 ttaaaggtga atttttaaat gctaaacata tgagaataca caaatccata ttctcctcaa
     7501 ggtcagacct taaaataatg aataacaaat tagaaaatta tattacaaat gtacttgaac
     7561 cattattaac attaagttat tcacttggaa atgaataccc tcatctagtt ataaaagata
     7621 tatggaaact tatgtttgaa aatgcagctc atgacagcat aggttcttgt aatagtgaca
     7681 caacaaatga ggatgtatac acaagataca aacaagccag agatttgagt ataaatttat
     7741 tagatttgca tatgagactt ataagtactc aaatcaaaaa taataatgaa gaaataacat
     7801 taacagtatt caatggattc ccagaagtca gaaatgaagt tgtagaattt gatacttata
     7861 ttccaggtca agattttgtg ataaagaata aaaatggaga aattcttaat tatataataa
     7921 aagaaaaaga agatattaca aattatatat taactcaaac aataagatta aacccaagta
     7981 aaaaaatata tatacccaaa actgtatata gagcaaaaat tgcactttca gtaaaagata
     8041 taccaccaat gggatatact caactagttt ttgaattgga tactaaaaat aaatgtgaaa
     8101 taaaaacaag taattgcaaa gaaatagaaa acaaatacta caaaataaca gccaataaaa
     8161 atggttcatt agaaatatta gataaagaat ctaataagac ttataaaaac caagctgtaa
     8221 ttgaagacaa tggagatgat ggagattcat acaactattc tcctccaagg aaagatattt
     8281 atgtttcatc tttagattct gtatcttcag tagaagtgct aaaatcaaac attcaagaag
     8341 aaatgatagt taaatattca ttgaccatac caacttcact ggaagaaagg gctattggta
     8401 aagtttctgt gaaaatgcca gtaacaatga aaatcacctt agaacctaaa gaacaaataa
     8461 ttaagtttaa tgttaatata aaaaataacc aagcactatc tcatagagta agagttttat
     8521 ttaatacaga aatagcatct aagttttcta tagcagatca acaatttgga ataatacaaa
     8581 gaccaacagt attagaaaaa gaccttgcat tatggaaaag agacaattac tcatggcaag
     8641 aaaaacctat aactatcgaa ccaatgcaaa gttttgttac tttagaggat ggacaaagag
     8701 gaatagcatt aataactggt tgtgtaagag aatatcaaat agttggtgat aatttagata
     8761 caatagcatt aacactattc aggtcatttg gatatatggg aagagaaaat ttactttata
     8821 gaccaggaag agcttctgga gaaaaaataa tagcaacacc agatgcacaa ttacttaaag
     8881 agttaaactt tgactttggt ctttacatat ataacaataa atttgatgaa gcaaatgtag
     8941 ctaatacagc taagagattc ttaacaccta tacaaatata tgagtatgct gattttttaa
     9001 atggtagact tatatttgca ttcaatgacg aaaaacaaat atatgaaaat gaatatagtt
     9061 tgatgaatgt aaataattca aatttcacag taagtgcaat aaagaaagaa gaaaaaggag
     9121 atggaattgt agttagaatc ttcaatggta tgaaaaatga ggatgctaag ggaagtctta
     9181 acatcaataa aaatgtcaat gatgcttata gtgcaatgct cgacgaaatc tatgataata
     9241 ctagcaagct taaagtaaat actaaaactg tagaggtaaa ttctttatca cattgtaaag
     9301 tacaaacaat agtaattaaa taatataaaa tagcttatac aagatttcaa aataaattgc
     9361 tagtactatg ttaatagtaa agccagatat tatgctattt ataagctctg taacactaaa
     9421 taacattttg taaaataaaa aggtgaacct tgtatattag gattcacctt ttttaaataa
     9481 atcaacattc tgttttaaaa taacatttat aagctgtttt gaaataatgc ttacaaatta
     9541 ttttaaaaca atacctataa atttttattt aaagaataca cataaatatt tccagactct
     9601 tttatcctat agactttata attattaaag aagtctaatc taatttttgt taaaagtaat
     9661 tgtaacttta aataaatcac catccacact tatattcatc ttaccaccct gtaattctat
     9721 aaaacttttt gctatcgcta aaccaagacc agaaccttct gtgttacgtg atttatctcc
     9781 tcttacaaat ctctccatta tatcatctac actaaaatca atctcagatg cagacatatt
     9841 tttaagcata atatttactt tattatctgt ttctaaaata tcaatataca ctcttgaatt
     9901 tggcattgca tatttgctta tatttattat aagattttca aatactctaa atgtacgctg
     9961 actatctaat ggtaatatta ttttttcatt agaaaagtta ttctttacta ttagagatga
    10021 atctgcaagt ttgtcatcta attctaaaag tgtctgcttc attagagata caatatccac
    10081 atttactata ttcaaataaa catctccact atttgccttg cttacttcaa acaaatcttc
    10141 tattagatgt tgcaatcttt gtgactttct gtctaatgta tcaatataca acttacgatt
    10201 ttcatctgtt atactttcat cttttaataa atctatataa gtgattatag atgttagagg
    10261 tgtttttaag tcatgtgata catttgatat aagctctgtt ttcatctttt gactcttaac
    10321 ctcttcgtca acagcttttt taaatccagt ttgaatattt cctaattcgt cttttatagg
    10381 attaaaaaat cctaaatctt tttccatatt tacatctaag tttccatctg aaattttctt
    10441 tgtaacatct aaaagttgat tatattcata tattgtcttt ttagagtaat ctgacagtat
    10501 tttaaataat actactgaat atattaatgc gagtattatt ccaaagaacc acatactaca
    10561 cattataact ataatcaata aatttattcc caaaattgta actatagttt tatttggctt
    10621 ttcttttaga tttattgttc taatttgatt aataactgct tttcctgctt tttttagcca
    10681 tacaaaaaac attcctgtca cagtatgttt tgtaaaatac tctcttattc caacatttaa
    10741 aatatgtttt aaaagtatta taaatacaaa tattattgag aatattacaa accaataagt
    10801 taaattcatt aaacttacca atatactagc cattttagca cttatgtcat tgtttataag
    10861 gtactctaca taattcccac tcaaggtatt acttattata acagtaggat ataaatctac
    10921 aagcccctgt ataatagcaa gagcaataaa gagtatctct aacggtattc ttgataatgt
    10981 atcaaatcct agcaactctt tagattttct atatgggaat gctaaagcta caataacaac
    11041 tatagctaat ataattttta aaatcattcc agaaatctta taataagaat tattttctac
    11101 attgcttata tatgaaatta taccatcatc aaaataagta tttccataac catactctac
    11161 actatcacct ttacttatta ctttaggtac agcatatact aatgttatat tttttattgg
    11221 agataactta tatgaaattg aatttcctcc taaacttgta tccattaaat cttctcttgc
    11281 tttagataac aacatttgat taataatttt ttgatttact ccataagaat ccacaatatt
    11341 tacttttcca tctttatcaa aattcattac aacgtagaat ctatagtttt cttttatatc
    11401 tgtaatctgc ttttgtcttt cctcatcatt catttctgta ttttttgact gattttcatc
    11461 agaattttct tttgaagcat cattctttgt tgtatcaagt actcctaatt ctttattatc
    11521 attaaaaaat tcaaaattgc tttctttatc aactaaatag tactttaaat tctttcctga
    11581 cgctttaatg aaatgctccc aattttcaaa gttttcatta aaatctgcat taatactatc
    11641 atctgtataa taagtttctg aaccatcata agtatctttt tctttactta cattttttat
    11701 atagttcata gtttcttcat tgataaatag ctttgatggt tgtatcatct taccttcttg
    11761 ttcttccttc atattatgat ataaaacata acttccttta tatatactat ctacaaagct
    11821 ttcctgttca aatggattct ctgctccacc tttagcactt gcttgtatag tagaatattg
    11881 actaatcata cctagtgatg ctgataacat aagtataaaa attataatag taactatata
    11941 tttatcttta aattttttca attttgtatc caacacccca caccaccttt aaatatttag
    12001 gatttttggg atcaatctct atcttttccc ttatattcct cacatgtacc ataactgtat
    12061 ctgtatttac agcattttca ttccaaactc tttcatatat ttcatcagca gaaaaaactc
    12121 ttcctgcatt cctcataagt aaacttagta ttttaaattc tataggagtt gtttttatat
    12181 actttccatc aatactaact tcttttgtat tttcatttaa ttctaatccg cctattgcaa
    12241 acacactatc gtcactttct accttttgct ctttatttcc aaccatgttt agatacttag
    12301 tgtaccttct aagttgtgaa ttaactctag ccaatagttc taatggctta aaaggttttg
    12361 ttacataatc gtctgctcct atattaagcc ccattatttt gtcaacttct tctgattttg
    12421 cagatagcat tattactgga aaattatagt tttctctaag cttcattgta agtgttatac
    12481 catccatttt aggcatcatt atatcaacta tagctaaatg tatttcttgg ttttctataa
    12541 ccttaagacc ttctaatcca ttgtatccct taaaaacatt atatccttga tttaatagat
    12601 atatctctat agcatctgct atctcctttt catcttctac caccaaaata ttatatgaat
    12661 tcattctaat ctcctttata ttaatttata aattttcttt acatatatga ttatacaata
    12721 tgatagaagt ttttccattt atatatatat ttatatcttt tgttctggtt atttgtattt
    12781 aatgtccatc ttagaaacct ccttttagtt t
//
LOCUS       DAEMHW010000109        12806 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEMHW010000109 DAEMHW010000000
VERSION     DAEMHW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.109"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            36..584
                     /locus_tag="KPK67_002487"
     CDS             36..584
                     /locus_tag="KPK67_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2459438.1"
                     /translation="MGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKADIEPVL
                     LKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEERLSEFI
                     NGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGVLKILERLNIK
                     TFLTSNGEVVINSDGKTLSVNQ"
     gene            702..893
                     /locus_tag="KPK67_002488"
     CDS             702..893
                     /locus_tag="KPK67_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459439.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            1116..1466
                     /locus_tag="KPK67_002489"
     CDS             1116..1466
                     /locus_tag="KPK67_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459440.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            1728..3233
                     /locus_tag="KPK67_002490"
     CDS             1728..3233
                     /locus_tag="KPK67_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBG2459441.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            3623..4720
                     /gene="dinB"
                     /locus_tag="KPK67_002491"
     CDS             3623..4720
                     /gene="dinB"
                     /locus_tag="KPK67_002491"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBG2459442.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKSSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            4983..6119
                     /locus_tag="KPK67_002492"
     CDS             4983..6119
                     /locus_tag="KPK67_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459443.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            6112..7659
                     /locus_tag="KPK67_002493"
     CDS             6112..7659
                     /locus_tag="KPK67_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG2459444.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVLVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMRHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKVNEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            7993..8859
                     /locus_tag="KPK67_002494"
     CDS             7993..8859
                     /locus_tag="KPK67_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG2459445.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            9000..10142
                     /locus_tag="KPK67_002495"
     CDS             9000..10142
                     /locus_tag="KPK67_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG2459446.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDVHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            10526..11764
                     /locus_tag="KPK67_002496"
     CDS             10526..11764
                     /locus_tag="KPK67_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459447.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            11793..12680
                     /locus_tag="KPK67_002497"
     CDS             11793..12680
                     /locus_tag="KPK67_002497"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBG2459448.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALTSVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            12714..>12806
                     /locus_tag="KPK67_002498"
     CDS             12714..>12806
                     /locus_tag="KPK67_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBG2459449.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNK"
BASE COUNT         4928 a         1321 c         2190 g         4367 t
ORIGIN      
        1 gtaggttcag tttaactcat ttagataagg accatatagg tggagtagat agtgtgttat
       61 ctagtgttaa agtaaagaat attatacaag caaattataa gaaagatagt aagcaatatg
      121 atgaatatat tgactcactt aaaaaagctg atatagaacc agtgctacta aaagataata
      181 tgaatattgt aatcaatagt gcagaaataa acatacatcc ggcttcaaaa agcaagtacg
      241 agagttcaaa tgattattca ataatcacta atataagcta tggagcacat aagtttttat
      301 ttgcaggaga tgcagaggaa gaaagattgt cagagtttat aaatggaaat actttaaaat
      361 atgattttgt taaaatgcct catcatggaa gatatgacaa attaactgaa acatttttag
      421 agtctatttc accccaatat gctgttatta catgttcaga gaaaaaagaa ccagaggaag
      481 gtgttttaaa aatattggaa agattgaata taaaaacatt tttgacttct aatggagagg
      541 tagttatcaa tagtgatggt aagacattga gtgtgaatca ataatttttt ttaaaatatc
      601 taaaaaaatt taaaaaaatc attagcaaat attacccctt aaacatagta tattttagga
      661 gctgaaactc cacatacaaa atgactagga ggtaacatat tatgttggat agattttttg
      721 gtgaaggcgg atttggtgga tgtgcttggt ggataataat attattcttc ttattcttag
      781 cattccaaga ttgttgggct gaaatggact tatgtgcatg gatacctttc ttaatattat
      841 tattaatact ttgtgaatgt ggtgacgttt ttgatggagg atgtggatgc taaaaaaatc
      901 aatttagatg atttaataaa gcttatggtg ggtataaata aaataccaaa ccaatcacaa
      961 tctagtgttt gaaggtagtc ggaaatcccc cgctaccttc ttttttttat tgttaaaatt
     1021 tatttttgaa ttatttcatt tacatataat aatttttacg ataaagtctg atataattaa
     1081 aaagaaaata aaatttattt ataggagatg taaaaatggc tatagtggat aagattttca
     1141 atgagataaa acttatggtt gcaagacttt tattgggaaa aaaatacagt gaatatgaag
     1201 aaaaagattt agtgtataat acagaagaag aagttatact tataacttta aaaagattag
     1261 tttttcaggg aaatgtaaac gaagcagaag aaattttatt tgataaagct aagagtgtaa
     1321 atagtgaaaa tatgcagtat atagcaatag aattttatac tatgcttatg gaaaagactg
     1381 atgaagaatt agaagctatg aatttttcta aacaagaagt ttatcaaggt atagaagata
     1441 ttagaaaagt attgaattta gaataaaaga tatatgaaaa tattgaaata aattaatgct
     1501 aaggtgagaa tattatactt atatataaaa atataatatt cttattttat tagttatatt
     1561 aggtatatct tatttaccat aaaactatct tgaaaaaaat taatcttaat tgtataatat
     1621 tactttgagt gaaaaagtat taacattttt ttataagatg ttttactaaa aaaatatata
     1681 taaatatact gttatcaata agaaatttaa tttagtgagg tgttaagatg aaagtaggat
     1741 ttgaccacaa aaaatattta gaagagcagt ctaaatatat cttagaaaga gtaaataatt
     1801 ttgacaaact ttatttagag tttggaggaa aattgatggg agaccttcat gcaaaaagag
     1861 tacttccagg atttgatgaa aatgcaaaga taaaagtatt acaacatatg aaagaaaaag
     1921 ttgaagttgt aatttgtgtc tatgcaggag atatagaaag aaacaaaata agaggagact
     1981 ttggtatcac ttatgatatg gaagttttaa gattaataga tgatttaaga ggttatgaac
     2041 tagatgtaaa tagtgtagta attactagat ttgaaggaca acctgctaca actgtattta
     2101 taaataaatt agaacgtaga ggaattaaag tttataagca ttatccaaca aagggatatc
     2161 cgtcagatgt tgagactata gttagtgatg agggatatgg agcaaatgaa tatatagaaa
     2221 ctacaaagcc aatagtagta gtcacagcac caggacctaa tagtggaaaa cttggtactt
     2281 gtctaagtca gttgtatcat gaaaataaaa gaggtaatgt agttggatat tctaagtttg
     2341 agacattccc agtttggaat gtacctgtaa aacatccttt gaatatagct tatgaagcag
     2401 ctacagttga tttaaaagat gtgaatatga tagattcatt ccaccttgaa acgtatggtg
     2461 aaatgtcagt aaactacaat agagatttgg aattatttcc agtgcttaaa aaaataatag
     2521 aaaagataac tggaaaagaa tcaattttcc aatctccaac tgatatgggt gttaatagag
     2581 ttggctttgg aataatagat gatgaagtaa taagaaaagc ttctatagaa gaaataataa
     2641 gaagatactt taaaactgcc tgtgagtata aaaagggaca agttgataaa ggtgcttatg
     2701 atagaatgaa aatgattatg gaagagctta acttaaagcc agaagataga aatgttgtat
     2761 tacctgctag aaactatagt aataaattga aagaaagtgc tgacaagaat gatacttgtc
     2821 ctgtagttgc attagaatta gaagatggta caatattaac tggtaagggt tctgatttaa
     2881 tgaatggaac tgcagcagta gtattaaatg ccattaaaca tttagccaat atatctgatg
     2941 atatgcactt aatatcacct gtaatattag aacctatcat aaacttaaaa acaaagactt
     3001 tagctagtag aaatgtttca cttagttgcc aagaggtgtt aactgcactt agtatttgtg
     3061 ctgtaactaa tccaactgca caatttgcta tggaaaagtt agcattatta aaagggtgtc
     3121 aagctcattc tactactata ttaaatagag atgatgaaca aacatttaga aaacttggta
     3181 tagatgtaac ttgtgaccca gaatatcctt cacaaaatct ttactatagt taaaattata
     3241 taaaaaaata tgggctgttt taaagtaata gtttgagcag tccattacaa ttataataaa
     3301 atcagtaggc tgtcttgtat aagacagtct tttgttttta aaacatccca taaattaaat
     3361 atatatactg ttatccataa ggaattttaa atttagttcc ttggaatacc tattgtaaag
     3421 cttttatata gtttttgcaa gtgtttttaa ggatttttta taaattatat tgaagttaat
     3481 ggattataga tggttttatt tatggaaaat atgaatatat aaagaaaata atgaaaaaat
     3541 accaaaaaga aacataagtt tggtataatt aaaaagtaat ataaataaaa ttaatctatt
     3601 tatactttaa aaggtgataa taatgaaaca ttatagaaaa ataattcata tagatatgga
     3661 cgctttttat gcatctatag aacaaagaga taataaaaaa ttaaaaggaa gacctgttat
     3721 tgtcggtgga aatccacaaa gccgaggtgt ggttgcaacg tgttcttatg aagctagaaa
     3781 atttggtatc cattcagcta tgccatcagc tgtagcttac aataggtgtc catatgcagt
     3841 ttttgtaaga cctagatttg atgtttataa gagtgtgtca gagcaaataa gagatatatt
     3901 ttatagatac acggatttag ttgaaccatt atctttagat gaagcttact tagatgtaac
     3961 aaaaaataaa aaaaatattg attcatcaat tgagatagca aagcaaataa aaaaagatat
     4021 atttagagaa gttggtctta cttcttcagc tggtgtgtca tacaataagt ttcttgctaa
     4081 gattgcatca gacttaagaa agccaaatgg gttaactgta ataaccgaag aaaatgccca
     4141 ggacttttta gataaattac ctgtgaataa attttttggt gttggaaaag ttacttcaaa
     4201 tactcttaaa aatttaggta taaaaactgg atatgattta aggtgtttaa atttatttga
     4261 acttgaaaat atattcaaaa aaagaggtta tgaattatat aaatttgcta gaggaataga
     4321 cgatagacca gtggaaccaa atagggttag aaaatctgtt ggggctgaga ctacattaag
     4381 tcataatttg gatattgatg aagaagaaac acgcaacatt cttgatgaac tttgtgaaga
     4441 agtatgtcat agattaaaaa gctctgagaa gtttggaaaa acattaacat taaaaattaa
     4501 atatgaggac tttacaaaga taactagaag ccttagttta gaacactata ttgacgaata
     4561 taatgatata agaagtggtg tagataattt gttgagaaat gttgaggtta atggaaaaca
     4621 aattaggcta ttgggagtta caatatcaaa cctaagtgat aaaaaagaaa cttataaaga
     4681 tattacatta tttgaataca tggatagtat ccaaatgtga gttttgttta aatgaaataa
     4741 cactagcttt aatactatat aattgaggct agtgttattt tatgaatatt tttatatttt
     4801 ttgttttttg acattatata ttatatacta tatataaata ctttatattt gtagcaatat
     4861 tcagacaaat agtgacctaa aaatcgtact ttgtttacat aaataaaata gtatgatata
     4921 attataaaaa caacttataa tattggagaa ggttgcttgt tcgaaaaagg ggatgataaa
     4981 ttatgataga gataagaaat gtaacaaaaa aaataggaaa taatgttata ttagatgata
     5041 tttctctagt tgtagaaaca ggtacattgg tagttttgat tggttcaagt ggttgtggaa
     5101 agactactac tttgaagctt ataaacaaat taataaaacc tacatctggt gagatatata
     5161 ttaatggcaa accaatttct caggaaaatg aaatagagct tagaagaaaa ataggatatg
     5221 ttatacagaa cacaggccta ttccctcatc ttacaataaa agaaaatata gagctaattc
     5281 cacgcctaaa aaaggaaaaa tccgttgaag aaattgaaaa aagaacgttg caactacttg
     5341 agatggtagg acttgatagt gatgaatttt tgaataagta tccttcagaa cttagtggtg
     5401 gacagcaaca aagaatcggt gttgcaagag ccattgctac tgatgctgaa ataatattga
     5461 tggatgagcc ttttagtgct ttagacccaa taacgagaac atctttacaa gaacaattat
     5521 tttctcttca agatgaattg aaaaaaacta taatatttgt aacgcatgat atggatgagg
     5581 ctttaaagat agctgataaa atttgtatta tgaaggatgg aagaatagca caatatgata
     5641 ctcccgaaaa tatacttaga aagcctgcaa atgactttgt aaaagatttt atcggagaag
     5701 atagagtttg ggataatcca gaatatataa aagcaagaga tattatgata aaaaatccta
     5761 ttgctgttaa ttctactaga actgtaactc aaggaataga aattatgaga acgagtaagg
     5821 ttgatagctt actaattata gatagagcaa aaacattaaa aggtattgtt actgtaaaag
     5881 atatgaaaga tattgatgat aagtcaatat tacttgctga tattatgtca agtgagcctt
     5941 tgcatgtaaa tgaaggtgat aatttagtag aaatactaaa tgtaatgaat cgtaattcgg
     6001 ttggatatat tccggttatt tctgatgaaa ataaactggt agggcttatt actagaagta
     6061 gtttactttc tgttttaagt gaacaattct tagaaatgga ggtgagtgtt cttggctaat
     6121 ttttttaact ttatactatt acaaaaagat aagattatag agcttttaat acaacatatg
     6181 agtctaactg ttacatctat tttaatagct ataatagttg gtgttcctct tggaataatt
     6241 atttcaagaa tctcatctct tagaaagttt gtacttggat ttgtaaactt agtccaagca
     6301 gttccgtcta tggcactttt gggtcttctt gtacctatac ttggtatagg aagtaaacca
     6361 gccatattta tggtagtggt ttattcatta ctaccaattg taaaaaatac gtatataggt
     6421 ataacaagta ttgacccagt agtattagaa tcagctaaag gtattggtct tacaagaaat
     6481 cagacattat ttaaaataca attcccttta gcacttccta taataatggg tggagttaga
     6541 atatctgcag ttacagctgt tggtttgatg actcttgcag catttatagg tgctggtggt
     6601 ttaggatatt tagtattttc aggggttcaa actgtaaata ataatatgat tttagcaggg
     6661 gcaatcccat catgtataat tgcactgtta gttgattatt tatttagtaa aattgaagtt
     6721 gcagttactc caaaaggatt aaatccaaaa gcacctaaga agaattatat agcacttaaa
     6781 gtaatatctg tagtgcttgt tgtttcaatg ttgttcgttg tttttagttc tagtttttca
     6841 agtaaaaaag atacaataac tattggttct aaagattata cagaacaact tatacttggc
     6901 aatgtttatg ctgaattagt agaaaaaaat acgaatctta aagttaagaa gaatttaaat
     6961 ctaggtggtt catctgttgc atttaatgca attaaatctg gtgagttaga catgtatgtt
     7021 gactatacag gtacactttt ggtaaatgta atgagacatg ctccgattaa agatgctgat
     7081 gaagcatata atgttgtaaa agatactatg gaaaaagaaa atcagttaac tttacttgac
     7141 cctcttggat ttaataatac ttacacatta gccatgatgc ctgaaactgc tgaaaagtat
     7201 ggaataaata ctatttctga tttgactaaa tatggtaaag aatttacatt ctcaccaact
     7261 ttggagtttg aaaatagaga agatggtctt gtaggacttt ctagagatta tggtttaaaa
     7321 ttcaaagatg taaaagcaat gaatggaagt ttaagatata cagctttaga taataatgaa
     7381 tctcaggtta tagatgcatt tttaactgat ggtcttatta aaaagtttaa tcttaagata
     7441 ttagaggatg ataaaaactt ctttgtacca tattatgcag caccattagt gagagaagat
     7501 actttgaaga aatatcctga gttagaaaaa gttatgaata tgctttcagg aaaagtaaat
     7561 gaagaaacta tgagagaact taactatcaa gtggatgaac ttggtaaatc accagaagaa
     7621 gttgctcatt cattcttagt taaagaaggc ttagtataaa aaattaatag tttatatcaa
     7681 aaatttaagg gagtgtctca aaatgagctt tttagttcat gaggcactct tttttatatg
     7741 aaatcaaata tattttatca tttcaacaat aaaaaagaag attatcttta ttttgagaca
     7801 gtctcttatt tttatgtata ccaaaaccat acctacgatt tttatttata attaagtttc
     7861 aaatagaatg aacaagtaca accaattttg aatataaata tagtaaatgt gcttatatac
     7921 gattttattg tctggttgat tgatattgat agatagcttt ctttattgaa tcgaagggag
     7981 ggggaatttg ttatggataa ctataagaaa gtgaagcaag tattgtggat aattttattt
     8041 gctaattttg cagtagcatt attaaaaatc attattggta atcaaataaa aagctatagt
     8101 atgacagcag acggatttca ttctttgtca gatggagcat ctaatatagt ggggcttata
     8161 ggtattttct ttgcatctaa accaaaagat aaaaatcatc cttatggaca taaaaagttt
     8221 gaaattataa ccagtttatt tataagtggt atgttgtttg ttatagcaat taagataatt
     8281 cttagtgcag tgttaagaat agctaatcca gttgtacctg ctataactat agaaagttta
     8341 attgctttaa ttattacact ttttataaat atttttgtat gcatgtatga atatagaatt
     8401 gggactaaac taaatagtta tgttttgatt tcggattcat tgcatacaag aagtgatata
     8461 tttgtgtctc taggtgtatt ggttacttta gttggtgtta agttaggatt tccagtaata
     8521 atagaatcta tagttcctat tattatttct gcattcataa tttattcggc ttatggaata
     8581 tttaggccat ctattggaat tttggtcgat agagtggctg tagatgagga ttatataaaa
     8641 gaaattgtgt ttgaatttaa tgaggtaagg gatgttcaca atatcagaag tagaggtagc
     8701 aaaagttcta tttatattga catgcatgta atggtagacc catttataag cgtagagcaa
     8761 tctcatgatt taacacataa aattgagaaa caaatacaag aagaaattaa tgaaaatgct
     8821 caagtaatag tccatattga acctttttat agtttttaac ttagttataa aatacaagtt
     8881 ataaaaaaat ataatttata aaaaaaatat tataccataa ataatttata aaaattattt
     8941 atggtataat attttctata aagaaattaa gtcttaatat atctaaggag gaatttacaa
     9001 tgagatacgt tgaatatgga aagacaggga aaatggtttc agtagttggc tatggaggat
     9061 taaggtttga tttagaaaaa agtaatcaag aaaatgctga tttagtcaaa tatgcgtatg
     9121 aaaaaggtat taattacttt gatacagcac cagggtattg tgatgacaga agtgaagata
     9181 tatttggact agcctttaga caaatgataa aggaaggtaa gaccgatttt tatgtatcaa
     9241 ctaaagaaaa gccaaaagca tgtgatacat cagaaaaagc tatagaggct gtaaaaaaat
     9301 caatagaacg attaggcgta tctaaaataa atttttatca tgtttggaat attagaaaga
     9361 tggaacatta cgaactagca atgagacctg gaggtcaata tgaaggatta ttgaaatgta
     9421 aagaagaagg attgatagac catatagtat tttcatctca tcaaccagga gatgaggtta
     9481 tagatattct aagtgaaaat aagtttgaag gtgtaactat gggaataaat atattaaact
     9541 ttccatatag gctaaaaggt gccaaatatg ctgttgataa tggatatggt gttgtggcaa
     9601 tgaatccatt aagtggtgga actattccaa agtataataa agaactttct tttctatcaa
     9661 aagatggcga aagtactata gaatcagctt taagattcaa cataggaatt tcacaaataa
     9721 gtatatcttt gatagggttt aataaaaaac aagatataga tgatgcatgt aaaattgcag
     9781 acgaaaatag aatttattca gatgaggatg tacatgatat tgagactaga ttaaataaga
     9841 atatgaacga aatatgtaca ggttgtggtt attgtaaagt atgtccaaaa ggtattaata
     9901 ccccagccta tatgcttttt tataatgaaa agcagatgtt taaaaagagt gatgaagaaa
     9961 tgacaaagtt agtttatggc ttaggacatt ggaattacac aatgaatagt aaggcaaaag
    10021 ctaaagaatg tatatcttgt ggaaaatgtg aagttgaatg tactcagcat ttacctataa
    10081 tagatagatt aaaagaaatt aaaaaatggg aagaagatgg agctaatagt gttaaagtct
    10141 aattaatttt atactatatt tgaaatttgt atagtattta gtaatgtttt aaaatatagt
    10201 tttaatattt aaggagacag attataattt tgtctcctta aattatgctt taatttgtta
    10261 tagtttaatt ccataagcaa cattgtatat ttttatataa agtttttcaa atattttaca
    10321 ttaatattgc ttacaaattc tcattttata caacactaat ctatgttata taaacaatac
    10381 ttgactgatt ataacatcaa taaagtaaat attgtggttg taaataaaga gttattcaat
    10441 tacaataaaa caagtaagaa cattatgaag atggctatta aatataagga ataaattgta
    10501 ggtgtcagca aatcctatat aatgaatgga tggtgatata ttgatgagta aaatattaaa
    10561 atataaaaac gagatgtttt tgtttatatt agttgtaata acttacttaa taataactaa
    10621 gatatttttt tctaagacta ctatatttta tgatttaaac aatacttatg atgtattgtt
    10681 agatacagat acaggagttt tattcaattt aaatgtattt gctataagtc aagacaatag
    10741 taagcacata ttgttttcag caattatatc aatttttgca tatccaatat acctattttg
    10801 tacttcaata gcaaatcctg ggacaacaga ttttaatagt gcctatgggt ttggactaat
    10861 ttgtctacaa ataataacta gtgctatgtc aataacttta gtctttaatc atattaaaaa
    10921 gataaaaatg cagagattaa ctttaatttt acttactatg ataatgatat tttcttttcc
    10981 acagttattt atgactttaa atgtagagag atttatatat agccaatttt cattaatatt
    11041 tttcatagtt attgcaaata agatgaaagg aaaaaattct tatttagtag aattagcagc
    11101 tattcctcta tttggaataa ctattagtaa tatatatttg tattttttta atatgatatt
    11161 tgagtttaaa ttaaaaatag gaaaaatgtt aaaacattta attacattta tcctaatggc
    11221 atatatttgt gtagtttcaa caaagtctta tgagagtttt atgaaccttg gaaatgtaat
    11281 acagtatgat acgaaattta taagtggaga accaattttg gaaaagattg caatgattat
    11341 agagagatta ttgtattcgg ttttttattt tcctggagca agtattaaaa aagggttatt
    11401 tttgcaaaat ggagaagttg ctacaatacc agtaattctt acattattag ctttatgttt
    11461 ttgtgtttta tcagttattg aaaattcaga aaaaagagtt cctaagctat gtatgggaat
    11521 aattatattc aatctaactt tacatggtat agtaggatat aatctagtca attcaagtat
    11581 tatggctata aattttagtt ttgcagttat aattttattg gcttacttta cgaaagcgct
    11641 tagaaaaaat gaaaagaata tgtataatat atttctatcg ctgttattgg ttactatagt
    11701 aatcagtaat gtcaatggat ttatagaaat tttaaacata gggataaaat catatcctgt
    11761 ttaaaaaata atattgaaag ggtagtaagt ttatgaaaca gaccaataca agtataaggc
    11821 ttcttactaa aatttcagat tatataaagt taatgagaat aaagcaatat ataaagaatt
    11881 tctttgtatt ttcagccata attttttcaa acaacatact aaatataaat ttatttttaa
    11941 atacgtttat tgcgtttgtg tgtttttgcc taatgtcatc atcagtatat gcactgaatg
    12001 atgctataga catggataaa gataaaaaac atcctaaaaa atgtaataga ccagtggcaa
    12061 gtggaagaat aagcaaaaaa agtgctaata tactatttgt agtattagct ttaacttcag
    12121 tctgtttatc aacagttgtt agttttaatc tttctataat tctcagtctc tatttgataa
    12181 acaatatatt atattctctt aaactaaaga atataatact attagatgtg ttttcaatat
    12241 ctcttggttt tatacttagg gtttatgcag gatgtgtagc cataagtgtt agtttatcaa
    12301 attggataat tttatgtaca tttttcttat ctctgtactt agctcttgga aaacgtaaaa
    12361 aagaaataga aactctaaga gatgatgcag ttgaacatag aaaaatattg gaagattatg
    12421 acatagaaaa tctaaatcaa atgatgatag ttatattaag ttctacaatt gtatgttatg
    12481 cattgtattc tacaagtaat ccagaaaagc cacatatgat atttacaaca atatttgtag
    12541 tttatggtgt acttagatat aattatataa ttaacactac aaatgaaaat aatcctactg
    12601 atattgtcct aaaagataat gctttaaaaa taaacgtaat cttatggata attacatgtt
    12661 taataatatt gattttttag tgatattttt agtgatagga tggggaaata cttatgaaaa
    12721 aaagtgtatt attacttgat gtagattata ctgttataaa tactgatagc atgatagatt
    12781 tttttattta ctctttaaaa aataaa
//
LOCUS       DAEMHW010000110        12735 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEMHW010000110 DAEMHW010000000
VERSION     DAEMHW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.110"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(209..2089)
                     /locus_tag="KPK67_002499"
     CDS             complement(209..2089)
                     /locus_tag="KPK67_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBG2459450.1"
                     /translation="MNKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKVTINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGSSGNDGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGTDRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVVVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNETEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(2299..8262)
                     /locus_tag="KPK67_002500"
     CDS             complement(2299..8262)
                     /locus_tag="KPK67_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBG2459451.1"
                     /translation="MKKAISCVLAVSMCSVPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDESDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEETPKEEGNISKDDNSTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFQTYNASEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPINN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGFMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDANTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPEAETRKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQAKYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNAIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8386..8471)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8643..10046)
                     /locus_tag="KPK67_002501"
     CDS             complement(8643..10046)
                     /locus_tag="KPK67_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBG2459452.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASQNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(10508..12403)
                     /locus_tag="KPK67_002502"
     CDS             complement(10508..12403)
                     /locus_tag="KPK67_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBG2459453.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKVNEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     VLTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         3530 a         2338 c         1404 g         5463 t
ORIGIN      
        1 tctttttaaa catattcaaa attgaattta tatttatata tataaaagat atctgcttta
       61 tttaaaatta aacttaatat agctcaaatt attatacatt ttaacctcaa tattattaga
      121 ttgttaagat aatgaaaata aaataagaat gcatttaaat atataacttt atatctaaat
      181 acattcttat tttctattta cttaatattt atttttcaat taaactctta atcttatcta
      241 ttacagatga ggctattcct ccacctactt caaacaactt tgatgaattt ttacctttta
      301 atgacgattc ttgtgattta tctaacgtat ttgtagcaag tataactggt gaattgtttt
      361 tagctgctaa tggtccaact gctaaagcgt ctataagttt gtcttgattt gcaactacaa
      421 ctccatctag cttagattga gagtagaatt tatctattac tttggcattc gtctcttgtc
      481 tagtttgacc tgccacccta ttgtttgata tatctttgtt tgctattttt ccaacttgtt
      541 caatagcctt gttagatatt ttttctactc caccaataaa atatatattt gacaccttat
      601 ttgatttaat aaattgttca gtttcattat ctacaccatc tttttgtgta agtactattg
      661 gagttttttc tttaccagcc aatgatgcta ttgataatga atcagcttct ccatttcctg
      721 ctcctatata caacttgctt acattgttcg tcttatctat ttcttttgct atattaatag
      781 aagtttgata tcttgtatct ccacctattc tttgtactga tatttttgaa tttacagctt
      841 tgatagcttc aacaactgaa tttggcattg agttgtttcc acctataaca actacttttg
      901 atggatttag ccttttcaat tcttctacag ttttttgagg aagcttttca tttgatgata
      961 aaagaattgg tgagtttttt acactagcaa gtggtgtagc tgtaagccca tccaccatac
     1021 ttttatcttc tccatttact attacaactg tatctgattt tgtccaacca ctttgagata
     1081 ttttgatagc tgtctcaaat ctatcagtac cttttaattt tttgactgtg acattagatg
     1141 tagaatcact tgaatttcct cctccagagc ttccaccact agatgagccg cctgaattac
     1201 ttcctccacc atcatttcca ctactaccat tatctggtgg taattcatcc ggttttgata
     1261 attcattttc aactatgtat tctaaacttt ttggaagtgt aacttcaaca tatttgttta
     1321 ttgtaacttt tcttccctta gttgtttttt gtgctaatcc aagttttaat attacatttg
     1381 aaccactttc ttgtgtttct ttactaacaa tagaatagtt tatcctaata ttgtttaata
     1441 aatattcaag tgcttcactt ttctctacct tatacttttc agggtcattt ttactagaac
     1501 cagttccacc aattggtttt acttcactct cattaataag ggtaaatatt aatccttcat
     1561 cagagcttat attttcataa tccactgttt tgttgttata tgttacaatt tttaaagctg
     1621 gtttatcgtt tgttcctttt cctaaaactt tatagtcagc atttgagtta ctgccagatt
     1681 tttttacaga tattcttcct atattcaatc tatttccgtc tagtaagtct tcatttgatg
     1741 taacatatat atctaatgta gttgtattac ttgatttgtt gtattctgct ctacaagtag
     1801 catttgcatt tccacttttt atctgagatt ttatattatc agaccaagta atcttttctt
     1861 tctgctcatt atctactact tcaacctcac cttgtacttt tatactggct tgaaacgaat
     1921 atactatgtt ttctactgag tccacagata tatctatagt atttactttc tcatacgttt
     1981 ttgttccttg taaaactgga gctgcatatg cacatgtagc actaccagtt acagctactg
     2041 ccatagttga tactgctata gttcttataa aagattttct tttattcatt aacacccgtc
     2101 cttatctgtc attaactaag tggataaatt tacttatcca cctagctttt atatttaatt
     2161 tataaattac cttaaactta tagttacatt tatatacttt acttttaata tctactattt
     2221 taaaactatt ctagtaccta atcttattag atatacaatg ttagtgttag tatttttacg
     2281 tttttagagt tccacctact atttatctct aacaatatta ccctttactt tattgcttac
     2341 agttataacc ttcttattac tgatatcaat agcatttctt gtagcttttc tagctttact
     2401 attactatca gtaagactta ttgtctttaa ctcatcataa ttcttagctc ctacaggtac
     2461 atacattgta tcacttacaa atctttgacc tactggagta tctccttgaa ggtcattgta
     2521 tctatatagt tcagccttaa ccttatcact taaagaatca aagttttctt ttggcatata
     2581 gtaaacccat gtacctttac tgatttcacc taattcacct ttttctggaa cttctgcaaa
     2641 taaaagtgct tctccagata tagtcttatt gtttttatct ataagtagtg gtaaattaaa
     2701 tgctggattt ccactgtatt cacctgtaat aactattgaa cccgctggaa tcttacctgt
     2761 ttcatcttca cttgtgctag agtcatcact ttgatataca taatcttctg ctaattttcc
     2821 aattccatta gtacgagttt cgtctgcatt attcattgtg ccaatttcta tattatcacc
     2881 tggaggtcct attatatcta attctgcaac ttctatatta gacttattat ttgagattag
     2941 ttttacatat ttagcttggt atgtccaaag tttaccatct ttattaaatg taactctagc
     3001 ttcattatct tggtcttttc ctccaccaac taaatcttgt ccatattcaa aagttccttg
     3061 acttacatct tcccagttct ttttatcatt acttacttgt atcttgtaat tagttaaagg
     3121 attgtatgtt gtgctagtat tctttctaaa gttaaataat ccttttaatg agaactttct
     3181 agtttctgcc tctggttttg tatattttag tcctactata ggtaatgttt ctttcatgtc
     3241 tactattata tatggatttt cctctacatt tatatcaggg tctttttgag ggtctttgtt
     3301 ccactctgct ttacttatta tctttccttt atatgtagta tttgcatcat tatcttttat
     3361 attatcaatt tcaggatttt gtaatggtcc atgtgagtca ttttcagttc taacatcacc
     3421 gtcagaaaca gtatttgtta tgaatgacca tgctgattta tttattttcc cttcatctga
     3481 taattttaca gtaccaactt caaattcttc tgttggattt aggtgataat catatgcaac
     3541 taccttatat gtaaatgctc tgttattaag aggagatata ttatcagtaa atttagtctt
     3601 actatttcct tttgcatcca caaatccaac tgttctgtat tttacctgtg ttccattttc
     3661 accatctaaa taatttccat cacttcttat tatttcgtat cctagtatct tatctttatc
     3721 cttatctaca tctaggtcta tagatatttt attagattta actagagaac cactttgtat
     3781 tccatcagaa aaactagcac ttactttagt atcttttgcc atactagcta tattttcatt
     3841 taaaatcttt cttctagctt cgtcatttac atattgtatc tttcttgatt ctttttcgta
     3901 gttcttacta tccattatac tgtttaattc tggtgtaatt tctactcccc atgccttaaa
     3961 gtaatctctt aaatcttttt caacaacact tgatgccatt gctactagca tttggtcttt
     4021 tggtaaacta ttaattgggt cactacttga aagtgctctg tattttcttg aaatctttgc
     4081 atagaatgta tcattactta aatcagcatc tgagttattc tttaacattg aacccgtaaa
     4141 atcatcatca taagctagat gtaattgcca taacattcca agcctttcaa atacattgct
     4201 tggtatacct aagctatttg aagttacatg ctcatatatc ttatccattg ttgaaccttc
     4261 aagtcttgaa tgtgtcttat catcaaaggt ctgagcaagt aaagcaagta cattgttact
     4321 agtttcacta tatgtcatct ttccaatatc tgtaacatgt cctatttcat gacctattcc
     4381 ccatccaaat aagtttcctc ctgtagcttt tccactatca tctatttgga aaggtactcc
     4441 attcataaac cctggaactg aaccatattc tactcctaca tgtaatccag aagcatacat
     4501 aaatgctcca ataaacattc tttggtattt tatgttcata cgagattttg gagccatatc
     4561 atttttagtt aagttaaatt cattatcatc tacagttcca ttaccatcta aatcttgtgt
     4621 tttaaatact cctctttctg agtaaactaa atccattatt tgttcccatg caagcatact
     4681 attatataat ctatctactt gtgtatttaa atcagcattt ccttttgata tcccttgata
     4741 tacagctgtt gcaggaacat ttaatgttac tttatcactt tcaatatctg tagtgtttaa
     4801 cacaccagtt ttttctgaat atccatatga attaccaaca tttgttcctg gataatatgg
     4861 ttctcctttt acagcatctt tatatttagc taattcttct atatatgctt ttatcttttt
     4921 cttagattca gaaactcctg caggactctt atcatttatt gttccattta gattaagatg
     4981 tggtatctta gtagctcctg ctacacgtac ttttatatct cctccacttg gagtatcttc
     5041 agcgtatctt atgtataaac ttcctccaca ttctatatct aaactatgaa gtttgcttaa
     5101 ttgaattaca ttttttccag tgttaagatt tatttctcca ctcttatatg caccagattc
     5161 tccatagtac tgtgtatata tcaacttagg agtttttcca cttttacttc caacatatac
     5221 tgtgatttca tctcctgctc tagcagctac tcctaatgcc tgccagtcat tttgcatacc
     5281 taaattattg gaatttacag tatactgtcc gtttttatct ttatattttc ctaatttatt
     5341 tatttcttga tttacagtag ttacatcagt aagggtggct ctatcattat ataagtcttg
     5401 agctaatttt aattctctct caataactgt cttatatgga tgatattctc cactagcttt
     5461 atctggagta tttaatctat cttttaattc agttattttt tcttgagtta catcttcttt
     5521 taattcaact tgtaggtcat ccataaatag atttcttaca tcatcttcta tactgtcata
     5581 ctcataaaac ttaagttctg atatattaga cttatatgaa taagatgagc aaacacttac
     5641 ttgtatcttg gtagcagtta ttggttcttt taatctaacc attacatact tattattatt
     5701 tatagggtct gtctttgttt caaaaggaac atcccccaga ttgtgtaatt gaccattttc
     5761 atcccaatat cgtacctcaa agtcatttat tgtcttatca gaaacatcaa aacgaggtgt
     5821 agccataaaa gtatccatag tataagattt atcaaaagtt attattggtc ctctattcgt
     5881 tccctcagat gcattatatg tttggaatgt ccaatgtgtt gtaaagtccc catctactac
     5941 attgaattta tcaaatggat ttttatcact atattcactt ttgtcatatc ctgctggata
     6001 agtaacatct acaatgtgat ttgttggtat tccaaatcca ccattctctt taggagtatt
     6061 aattaatcta tagttaggtg ttgttggtgc atccatgctt tcagttgtac ctatatatgt
     6121 tttagacatt ccactagttc ccaagtggtt agtagctgtc attctaactt catatgttgt
     6181 attatcctta agaccttcta tctttgaact tgtcccacga ataagcttat cattgtattt
     6241 tatccaagac ccattagagc ctttttcttt atagtacaag tcaaaatctt tagcttgttt
     6301 atgatttttc cagcttacat caagagattt aaatttacct ttaacattta taccttctgg
     6361 tggttctggt tttgtgtctg gaacaacttg aaattctaat atactatctg ggctaaattg
     6421 tataggttta tagtttgaat ctacattttc tggtactccg tctaagtttt ctggtgttgc
     6481 atctttataa ccactagacc aatctccatt taaagattgt atgctaactc tatatagtcc
     6541 atatgcttct atatctctaa cttctattgt atttttacta gtttgtaatt ttttagtaga
     6601 taatactttt cccttactat caattttgtc tagtataagt tcgtatgctg atacatttac
     6661 ttcattattc caataaaagg atactttttc atttccaaca gcagtgctag tcgatatttt
     6721 atttatttta ggtatattca tctctggctt tggtatttct ttatatacat tgtttaaaaa
     6781 ttcaactttt gatatttctg caagatttct atcatttgct gttgtagctg ttatttttat
     6841 agttatcttt tttactgcta cttgtttacc aagatttatt actatatcgc tattttgaac
     6901 atctgcatat gtttctaatg ctgacctact atatgtagat gttgtagagt tatcatcttt
     6961 actgatatta ccttcttctt ttggagtctc ttcatctgaa ttaggtttat ccattgatga
     7021 attttcttta tcattatttt tatcttgatt ggtattagag tttacttctt gtttatcgct
     7081 attttcctgt tgtttatcat tattttcatc tttattattt gagttattat cagtctggtt
     7141 tatattttca tctttattat ttacttcttc ttgatttact tcttcttgtt cgttatttac
     7201 ttcctcttgt tgctcctcat cttttaagtt tttcatatat tctttgtttt cataaggaat
     7261 ctgttgaaca ttaccatctt tgtcagtaac ctcaactatt ccttttgctg gagcagtacc
     7321 ttctggagct tttatattga tgacttccat attaagtgct tctgataaag ctatatccat
     7381 tactgctggt ttttcctcag atatttcatc attttttgaa gatattgtaa ctccattatt
     7441 ttctccacta aataaatttt ctacattacc attaatatct acatcactat ttccttttaa
     7501 ttcaacctta cttgtatcta ctataggatt ggtagatact atactttcta ggctttttgc
     7561 atcttcactt tgtcctaaat ttttctgtac ataatctaag tcaacaatat ctacctttcc
     7621 atctctattt aaatcaaatt ctcttttatt tgaatctata ttctctaaaa ccttattata
     7681 atcactctca tctactttac cgtctccatt tacatcaccc attaagaata attcataatt
     7741 taaaggttta tttttattat ctacacttgc tctattgctt aataagactc tttgtgaata
     7801 acttgatata tcaatgtctt taatttcttt gttcttaaat ccatctccag agacttctac
     7861 attgtatttt ccaacaggta aattattaaa tacaatatta taatatgata tattctcatc
     7921 tttatttttt atttcacttc tacttgcaga taattttttt atagtatatt ctatattatc
     7981 tgtcccaaca tttactgttc ctgaatcttt ttctccaagc tttattattc cactattgtt
     8041 actatcatct tttaatcgta actgcatatt agtcatactt tctgactcgc tattttttat
     8101 tggcagtgaa aaatttaaat ctacttcaag actccccctt aacttttctg taactgattt
     8161 ttcaactgcc cttagcttcc cctctaatat aggttctgca aaaacgttta gtggtacact
     8221 acacatagat actgctagta cacaagatat tgcctttttc atgaaataac aatcctccct
     8281 taataaatat ctaatttttc atgaatttaa atactataag tttcagacaa taaaaaaaga
     8341 cactcaaaga gaatcttaat tataaaataa aaagacttta ctctttttag caactggctg
     8401 atatggcttt aaaagcttta atccctatag ttttgcgtca ttagatttcc ctaattttgc
     8461 catttaattt tcactacttt cttacccatt ttatactata tttaaacttt ttaatacaat
     8521 ttttttacat taaattataa aaaaataaca tttatattaa tttattaata taaatgttat
     8581 tttccattat aatttaatta tttatatagc ttccttacaa gcataatttt gtgtatattt
     8641 tcttattctt tatatcttcc ataaaattca tgtgtagcat atagattctt atctcctgtt
     8701 atagctatac caaatcccat tatttcatac cctttatgca acatattagt tccttcttct
     8761 ggatttgtct tccactcatt aaaaataaag tttcctatat catctgcatc tctatttgta
     8821 tatacaggtt tatctggaat tttatattta attatatttt caccttgaac tgctatatat
     8881 ccaggtttaa tctcactcca atctaagtat ttcaaaaaag tagaatatga attttttcca
     8941 tttatcgtat gagataaagt cttcttattt gccattaaat tagaccaatc atttgctaaa
     9001 ccctctaatc tgcttgctac agataacgct cttactccat tctcttttct atactgcttt
     9061 gtaagagcat atactttacc ctttattctc tctttaaatg ctctatcatt tggattgtaa
     9121 tataaacctt gttgattttg tgaagcttga tttatacatt gcttgataac attctgattt
     9181 atgcctccta gtacagtcaa tttgtctgca ccacttaaaa tactcttgtt actgccattt
     9241 tcaactaaaa ctacaggagc attttttgct attgtagagg cagccaaagc gtctgttaat
     9301 tgtaagcctt tacttagata aaattcttta ctatttttat aaaactcttg tataactatc
     9361 ttattagttt caaatctatc tgtgccaccc aatctaatag aatttgtgct cttaactaat
     9421 tcgtcgctga ttatttcact agaaccaagc gcataactct ttaagcctgt cgtgtcaaat
     9481 cccacactat ttccatctgt aagtattata ggtactccat ctctagcagc tactggtgat
     9541 gcacttatcg catctgcttc tcctgtaaat ccattggcta taattacttt atctattggc
     9601 ttaatttctt tgattttttc agctattttt atacttgtat caaatctatt ttcaccaaat
     9661 attctttgta caaccttctt tttggatagt aactgattct caacattttt acttattgtt
     9721 gattctgaac ctattatata cacagtcttt gctttgttta atctttccat agttgtgttt
     9781 ggaatctggt tttgctttgt catcaaaata ggcgcattta aggctccagc taaaccactt
     9841 gcacttagac catctgctaa gctattatct gagttgatta gtattgctgt attgtagttt
     9901 tgcttatctg ctatttttgc tgctgtctcg tatctgtcat tcccttttat ttgctcaact
     9961 gatgtcaacg catttaccat aggagtattt accattacta aaaatagtgt aagtcctata
    10021 gctaatatct ttttactatt cttcatatta ttttctaccc ccaatgtact tttcatattt
    10081 tttattagta ctcatactta tttttaatca aaagttacaa ttttgttacc atattttata
    10141 tatgatacca tagttattca tacaactcaa acaatatacc taaagttttt attctctata
    10201 aattcaaaac attataattt attttattta attaagttat aagttcaaaa taatttatgt
    10261 taataagatg tttaatcatt ataagaagta tctttttata ataatttttg caatatgaat
    10321 ttctcaacat tataatcaaa aaataaaata aggtatctca atctaattct aaatgagaca
    10381 ccttattttt attattttat cttgttattt gtatatttta taatatctac actatgtctt
    10441 acatattaac gcattgtata atcatatatt atctaaataa acttttaata ttaattccta
    10501 tatcttatta gttcttagta aataagttta ttttttctgc tattttttca atagcgtttt
    10561 tagctattcc ttcacctatt tgtactaact tagtagcatt cttaagtctt aactcaacta
    10621 cttcttcttg tgatttagaa agttcttctg tagttaatac tattggagcc tttgaactag
    10681 ctgcaagtgg tgctgctgta agtacatcaa ctaattgtcc atttccacct acataaccat
    10741 cttttgctac atatgcttta gttgcacctg catagtattt attgattact ttagcattag
    10801 tatcttttct attttctcca gctaatcttt caactttatt atttaaatct atatcaacta
    10861 atttgtcttc aacttctttt gatacagaat tttctccacc cactatttct aattcttttc
    10921 cttttaataa tttttcagca tttgcatcta attcgttacc tgtaactatt attggagcac
    10981 caaattgagc agctctagct gatatactca tagcatcagc ttctccattt ccacctacta
    11041 caaaagcagt cttaaatgta tcttttaatt gttttgctat ttctaaagaa gtttcaaatc
    11101 tatcttctcc agaaactctc tcaactttac ttacacctaa tttagaaagt ttttcttcag
    11161 tttcttttga tactttagat tctccaccta ctatatatat tttcttagaa gatagattac
    11221 ttccaagtac tcttaatatt tcagcttcta tttctgatgg tagtgaatct tttttagata
    11281 ataatattgg tgcatttttt tgagaggcaa gtggtgctga tgctaatcca tcaactatag
    11341 catcttctcc aactaaaatt atagcttctg cttctccatc tttgaatctc tctttactaa
    11401 cttctactgc tgttgtaaat cttgaatcac cagataaaac ttctactttt aattcttttc
    11461 catcttttac atcagttaat actttgtgta agtctctaac taacttatgg tttttacttg
    11521 aaacacttat tgtttctggc ttttcattta ccttgcttaa aactatatca aagcttgatt
    11581 tttctggttt atctttatca tcactatctt cttgttcttg agtaaggatt gcttcatttt
    11641 caaatgttaa agcatatcca tttttaactt tattaactat ttctcttcct aatgtagtta
    11701 atctaattcc atcaaataat tcttctgctg atatgtcttg taaatctgcg tttttaactg
    11761 ttatcgtatg taattctttt gaatctatgt cactataact cttttcataa ccttcaaaag
    11821 taccttcttc tgatgttatc acttttgaga aatctaaatg ttggtctcct acatttaatg
    11881 taataacatt tgctgaatct tttgctcttt ttacttcaac tgtatttgta gattttatag
    11941 ttgctgttaa cttgttatca gaatcttctg ctaaagttgc attgtaagca ttaactgcat
    12001 ctactatttc ttgagcagtt ttgtagtttt caattttata gtcaactact tttccaccta
    12061 acacttgatg tcctttatct tgaatagtaa cttttaaact ttgcccttct gttaaagagt
    12121 ttatcttatt ttgtaattga gttgcattag ttaagtttac attgtctact tttatatcgt
    12181 aaactctatc atttacattt gctcctgctt ttgtatcttc aaacttaact tctaatgctt
    12241 ttctaacctc ttcgattagt ttagcagaat ctttcgcact tactgtgtaa tttttatcaa
    12301 cattttctgt cacagtattg tctgcgaata ttggtgctgc tgcactcata actgtagcac
    12361 ctgccattac tattgccata cttctctttt tgttgcttaa catatttatg tcctccgaat
    12421 attatttttt attttttata ttcgtatttc ccacacataa ataatattat atataatatt
    12481 atttttcaat atattttatg tgtttacata atttactaac acaaacacta ataatatctt
    12541 cttgaacccc ttccccctgc ttagctctct ataattatgc tcaaagatag aaatccctat
    12601 tttatagtga ttttctacta aacaatcata tactaacaaa agtattcaaa ctccattttt
    12661 atatactact atgcactaat ttttgcttta ctcttattac ttaaatatat atcactaaat
    12721 taatatctat ctcca
//
LOCUS       DAEMHW010000111        12733 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEMHW010000111 DAEMHW010000000
VERSION     DAEMHW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.111"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(207..1457)
                     /locus_tag="KPK67_002503"
     CDS             complement(207..1457)
                     /locus_tag="KPK67_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBG2459454.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1489..2430)
                     /locus_tag="KPK67_002504"
     CDS             complement(1489..2430)
                     /locus_tag="KPK67_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBG2459455.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LIVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2670..3746)
                     /locus_tag="KPK67_002505"
     CDS             complement(2670..3746)
                     /locus_tag="KPK67_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG2459456.1"
                     /translation="MSKKLFIPGPVDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4119..4634)
                     /locus_tag="KPK67_002506"
     CDS             complement(4119..4634)
                     /locus_tag="KPK67_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBG2459457.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(4996..6066)
                     /gene="leuB"
                     /locus_tag="KPK67_002507"
     CDS             complement(4996..6066)
                     /gene="leuB"
                     /locus_tag="KPK67_002507"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBG2459458.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVEICQKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HRYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(6073..6564)
                     /gene="leuD"
                     /locus_tag="KPK67_002508"
     CDS             complement(6073..6564)
                     /gene="leuD"
                     /locus_tag="KPK67_002508"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBG2459459.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6579..7856)
                     /gene="leuC"
                     /locus_tag="KPK67_002509"
     CDS             complement(6579..7856)
                     /gene="leuC"
                     /locus_tag="KPK67_002509"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBG2459460.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(7813..9555)
                     /gene="leuA"
                     /locus_tag="KPK67_002510"
     CDS             complement(7813..9555)
                     /gene="leuA"
                     /locus_tag="KPK67_002510"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBG2459461.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9678..9924)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10072..10518)
                     /locus_tag="KPK67_002511"
     CDS             complement(10072..10518)
                     /locus_tag="KPK67_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459462.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGHDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(10539..11258)
                     /locus_tag="KPK67_002512"
     CDS             complement(10539..11258)
                     /locus_tag="KPK67_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG2459463.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(11376..12650)
                     /locus_tag="KPK67_002513"
     CDS             complement(11376..12650)
                     /locus_tag="KPK67_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBG2459464.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
BASE COUNT         3842 a         2447 c         1311 g         5133 t
ORIGIN      
        1 taaatataca aatacaatta tatgaatttt catcctcaat tatagcatag aatatgacta
       61 tctatatcct aaaattatcc atttctaaaa aaaaaggtaa ctattattaa gaatatcaac
      121 ttatagaatt tatattccac atacgatttt caatcaaaaa aacaagggct aaaaagtgaa
      181 ttgtgcactt tttagccctt tattaattat tcatttattt catgtaagaa tagaccacat
      241 cttactttag gttcaaacca tgttgactta gctggcatta tcttatttgc atctgctact
      301 cttattaaat cttcaatact agtaggatac attgcaaatg ctactttcat atcttcatta
      361 actctttttt ctaattcttc aagacctcta attccaccga taaaatcaat tcttttatct
      421 actctaggat tatcaattcc taaaatagca tcaataacat agtcttgtaa tatagaaatg
      481 tctaatgagt caacagggtc ttctatttct aataaatttt tatttgcttt cattttatac
      541 cacttatcac ctaggtacat tccgaaagtt ccttttttgt caggtttata tttttctttt
      601 ccttctattt cttctagttc aaaattttct tttattttat ttataaattc ttcctcgctt
      661 aaaccattta agtctttgat aaccctatta taatccaaaa ccattaaatt ctcatctggt
      721 gcaatcattg ctatgaagta attaaattct tcttttccag tataatttgg atttttagct
      781 ctcatttctt tggctacatt ttctgctgaa gctgtcctat gatgaccatc tgcaatgtat
      841 agataatcta atttttcaaa tttatctact aggttatcta ttatatcatc attatcaatc
      901 ttccatactg tatgctctat cttgtcatct gttatgaaat catataaagg ttcattatta
      961 tccataaaat cattgattat atcatctatc accctttgat gtttgtaagt tacaagtata
     1021 gttcccgtat tagcatcaca gtattttatg tgtttagttc tatcaatttc cttttctggt
     1081 cgagtgtact catgtttttt tattgttcca ttcaagcttt catcaactga tatacatgct
     1141 actataccaa cttgagctct accatccatg atttgtttat atatatatat acactctttg
     1201 tcatctttta ctagaatttc ttgttttcga aactcatcta aattttctcg cgcttttaaa
     1261 taaacttttt catcatataa atctatattt tcatctaaat caatttctga tttatctata
     1321 tgcaaaaatg aatatggatt cccttctgca attcttctag cttctttact attcataaca
     1381 tcgtaaggaa gtgctgctac tttatctact atatatttat ttggtctaat cgccttaaat
     1441 ggtttaatcg ttgccattac taatttcctc ctaattatac aaaatatttt ataatgctac
     1501 ccctattaga ttgttgaagt caagaaagaa atcttgaatc acatttacta tttcgtctcc
     1561 tattctttct tgagcttcta ctgtagatgc cccaatatga ggtgtaggag aaactcttgg
     1621 atgatttact aagtctaaat ttaatgtagg ttcttgttca taaacatcta tagcagctgc
     1681 tgataattta ccttcatcta atgcttttag taaatctttt tcatcaacta aaccgcctct
     1741 tgcacagttt attaaataag cccctttctt catcttattt atttcttttt cagttattac
     1801 tgccccctta ttcttatcaa atggtatgtg taaagataag aaatctgcat tttctaaaac
     1861 ttcttccata tcacagaatt tataattgtt aaagccttgc gcctcaccca ttatgtcagt
     1921 atatataaca ttcataccta ataattctgc tctctttgca acttcttttg caattcttcc
     1981 aaaacctata agtccaagtg tctttccatt aatttctgta cctttatact ttttcttctc
     2041 ccattttcca tctctcatag ttacatttgc agtatttatg aatcttgcaa gaacaaataa
     2101 ttgacctatt gtaagctctg caacagatat actgcttgca tttggagtat tcataacttt
     2161 tatgccatta gcttgagcat atttaacatc tatattatcc agacctactc cacctcttac
     2221 tatcaacttc aactttttac cctctaatgc tttatctatt acatctttag taactttagt
     2281 agctgaacgt actataagta catcaacatc tttaagttta tctcctaaaa catctttttc
     2341 ataaaactgc tctatcacat taaaatctaa ctctctcaat tttctcgctg cttctttctc
     2401 tattccatca gtaactaata tattatacat aacaaactcc tccaattttt attcacttaa
     2461 tttttccaat ttttattttt taaagtattt tcatcaataa aaaactttac ttaatttaac
     2521 tttctgttta tataattttc aaaatatttt tttattaaag tcatatcaaa ttttcctatt
     2581 gtttgttaaa aatttaatat gtaattttta taaaatatta tttattatat attctaaatt
     2641 tatcttaaaa catacatatt tttacaatat tatttattaa aacctaatat atcatcaata
     2701 ttatctaata attcttgtac atcttcgaca gtatagtcac ccatatgtga aattctaaaa
     2761 gttttttctt ttaaatctcc atagccattt gctatttgga atcctcgttc ttgtaatttt
     2821 gaatttaaat ttgatacact aataccttga gtattttcta taacagtaag tgtattggat
     2881 aagtgatttt cattggtaaa aatttggaag tgttttttag cccagtttct aactaagttt
     2941 gccatgtctt catgtctgtt gaatctgtta tctaggcctt catctaatat attatctaat
     3001 tggaaatcta aagcgaacat gtgagaaaga gatggtgttg aagggtattg gtagtttttc
     3061 ttgattaagt attcatacat tgctaacaaa tcaagatata ctcctctaaa aggtacttgc
     3121 ttagctctat cgattgcttt ttgagaaaat gtgcaaatag acattcctgg tggtaatccc
     3181 agtgcttttt gtactgatgt tatacaaata tctattccaa tcttatcaac ctcgactttt
     3241 attccaccgg ctgaacttac agtatctaca cagtagatta catcttcata ctttttaaca
     3301 acttctccaa tttcttctat tggatttctt attccagttg aagtttcatt gtgagttaca
     3361 gttattaaat cgtattctcc ggtagaaagc accttatcaa ccatttctgg cgttgttgcc
     3421 tctcctaatt caactttaaa tatatccgct gggacattat tagccactgc cattttgtac
     3481 catctatcac caaaggatcc acaggaaaat actgctgcct ttttggatgt acaggaacga
     3541 atggagcctt ccattaaacc agttccggat gacgtggata aaagtatcgt gttgtttgta
     3601 tagaatagtt tttgcatctt ttcacttata ctcttttgga gcatagatgc atcttttcct
     3661 ctatgtccta tcattggtgt agccatcttt tgcaagactt cttctttaac atcaacagga
     3721 ccaggaataa ataatttctt actcattata attcccccct ttttagtttg taatatggat
     3781 agcgttttta ctgtccttat ttttaataac tataatatca agctgaatta actatgtcaa
     3841 cataaaattt attttttata tataatttat tttatatatt ttatgaaaac attcattttc
     3901 taccttttca taagtctgaa ttttctgaat tattctctca aaaaaaatat actttttatc
     3961 tatataaatt aattttttta gtatgtaaat ttgacgctat tacgataaat ctttttagta
     4021 taactaattt atttttatct tgtattttta ttgtattttt gaattttatt ttttctaaat
     4081 ataaaattta tgttttaaaa tataaaattt ttatttaatc aaagttcaat atattccact
     4141 ttttctttcg attcttcaaa aaaccattct tcattagaaa aatcaccttc tgcaatttgt
     4201 ccaataggca ttttcatata ctttccagtt gctgtcactg ctatatctcc atttggtaga
     4261 attatttctc ctgtaccttc aaataatttt ctgttttctt tagttattct tccaacaact
     4321 tttatagtag catctgttgg tactggtttc ttatatttca actctaaaga tactgttact
     4381 ccccatattt ggtcatcatt tatacttaca gctcttccaa tagtctcatc cagtattgca
     4441 gcagacatac ctccatgaac tctcccagga taactttgat gccaatcctt tgtattacaa
     4501 attgacacaa gctctccatt ttcaagttca taaaaatctg ctttaagtcc tagctcattt
     4561 tgtgttccac aaactaaaca agatttacta ctattttgct tcttcaaaac tttatacttt
     4621 gccaaaaaat ccatctccta accgacatta gtagtcgctt aatattaaat aattctaaaa
     4681 tatattatat aatattaata aaatccctgc aaacttctaa taataaaaat aggctacttc
     4741 aatatgataa gtttaattta aagtattatt ttccaaaaaa taactttatc aaaatagaaa
     4801 ctcaaagtag atataagcct ctttttcgtt attaaaatag taaaaatata tttgacttaa
     4861 tataaaaaag agtgtctcat taactaataa agtttatttt gagacacccc tcataaaaca
     4921 aattatacta caatagattt ttactaattc tacaagttat attttaaatt atattattca
     4981 aattaaacta taattctaaa tttcatttac tattaattcg cccatttctc ttgttcctac
     5041 aacatttccc acaccattat agatatcaat agttctataa cctttattga gtacactttg
     5101 tacagcatct tcaatacact tagcttcttc ttctaagtta aaactatgtc ttaacatcat
     5161 gcttacagat agaatagtcg caattggatt tacaatatct tttccagcta tatcaggtgc
     5221 acttccatgt attggttcat acataccaaa actatcttct ctaagtgatg ctgatggtaa
     5281 catacctata gaacccgtaa tcatagatgc ttcatcagat attatatctc caaatatatt
     5341 attagtcact attacatcaa attggctagg gtcttttaca acttgcatag cagcattatc
     5401 aacataccta tgtgacagtt ctacatcttc aaactcttta gctaagtctt ccattatact
     5461 tctccaaagt cttgaactct caagcacatt tgccttatct acacttataa gttttttatt
     5521 tctatttctg gctatttcaa aagctctctt accaattctt cttatttcat tttcgttata
     5581 tatttctaca tcataagcat tttcgatacc atcaatgatt tttcttccac gttctccaaa
     5641 ataaatgccc cctgttaatt ctcttacaac aacaaaatca atgcctttct caactatatc
     5701 cgttctaagt ggtgaagctt cttttatact ttcatacatt gtagctggtc ttaaatttgc
     5761 aaataaaccc aatgactctc ttaattttaa aagaccagac tctggtcttt tatctccctt
     5821 acatttatcc cactttggtc ctccaacagc accaagcaat acagcttcgt ttttttgaca
     5881 aatttcaaca gttttattag gtagaggttc tccagtttca tcaatcgcgc atcctccagc
     5941 taatacatat tcacaatcga atctatgatt aaatttacaa caaattttat ttagaacctt
     6001 tataccttca tctataattt ccggtccaac accatcacct tttatgactg ctatattaca
     6061 attcatactc cactaccccc tatttgcttt tattgagtta attaagcctt catttaatat
     6121 aatattttgc ataaactcag gaaatggttc tgctttatat tcttttcctt ttgttatatt
     6181 ttttatcaca cctgtagaaa aatctacttc tatattatcc ccttcatcta tgttattggc
     6241 agcttcttca cattcaagta taggaagccc tatatttata gagtttctaa aaaatattct
     6301 agcaaaagta gaagcaatca cacaggatac tccactttct tttattacaa ttggtgcatg
     6361 ttctcttgat gaaccacaac caaagttttt agttgcaact ataatatctc ctttttttac
     6421 cttagaaata aatttatcat caatatcctc catacaatgc gttgctagtt ctttataatc
     6481 agctatattt aaatatctag ctggtattat tacatctgta tctatattgt caccgaattt
     6541 aaaaacactt ccattggcaa tcataaattt tacctccctt aaattatttc tgatggtttt
     6601 gatatttttc cagttatagc tgatgctgct gctacagctg ggcttgcaag atacacctct
     6661 gaatcaacat gccccattct tcctacgaaa ttcctatttg ttgttgaaat ggatttttct
     6721 cctgctgcaa gtatacccat atgtccccct agacatggac cacaagttgg tgtagaaact
     6781 gctgctcctg cctctataaa agtggtaata tacccttttt ctatagcttc aagatatact
     6841 ttttgagttc caggaaatat cataactcta actccatctg ctactttttt gcctttcatt
     6901 atctcagcta ctacttcaag gtctgatatt cttccatttg tacaagagcc tattacaact
     6961 tggtctatct ttatatcctt atctatctca tctactgttc tagtattttc aggaagatga
     7021 ggaaaggcta ctgtttcttt tagttcagct aaatctattt cgtatacttc atcatagact
     7081 gcatcttcat ctgcttcata tatatttaca tcttttgtca tagttgaaca cttatgtgaa
     7141 tttatatatt ccatagtctt atcgtctact ggaaaaattc cattttttgc tcctgcttct
     7201 attgccatat tacaaatagt aaatctatca tccatagata agtattctac tccatctcca
     7261 caaaattcca ttgacttgta aagtgctcca tcaacaccta tctcccctat tatatgaagt
     7321 attatatctt taccacttat ccatttattt ggtttattct tcaaaacaaa ttttattgct
     7381 ccaggaacct taaaccaaca ttttcctgta gccatacctg ctcccatatc agttgaacct
     7441 acaccagtag aaaatgctcc taatgctcca tatgtgcaag tatgagagtc agcacctata
     7501 attacatctc cagcagttac tatacctttt tcaggaagaa gaacatgctc tattcccata
     7561 tctcctacat caaaaaaatt tacaactcct tttgattttg cgaaacttct tgtaaattta
     7621 cattgttctg cactctttat atctttatta ggtgtaaagt ggtctaaaac catcactact
     7681 ttagtcttat cataaacatc ttcaattcct aatttttcaa attctctaat ggctactggt
     7741 gatgttatat cattacctaa aactacatct aaatctgctt cgattaattg ccctttctta
     7801 acttcattta atttagcatg tttggctaat attttctgcg tcatagtcat ccccattttt
     7861 tatttcctcc tcaatataac ttttatttat tgcattcata agtgcattaa tagctgctgt
     7921 atttatgttt tccgatatac ttacaccaaa atagacttca ttattgtgct ctattcctac
     7981 ataagctacc cctcttgaat gtgacccttc ctctaatgca tgttcccagt aatatctaaa
     8041 tttataatct ttcatgccac attgctttag tgcattatta aatgaatcta caggtccatt
     8101 tcctatacca acaatccttt gttcatttcc catataactt attcttgcag tcatctcaat
     8161 catgttagta tcattagaat catcattttc atagtttaat tcatccattg attttatttt
     8221 atacttctta actttataag ggctctcaac ttcaatatat tctttcttaa acaaattaaa
     8281 tattgcttca ctactaagtt cttccccaat tctatcagat tccattttga ctacatttcc
     8341 aaaatcagaa tgcatattct taggtatcat aaatccataa tctgtttcca ttatataaac
     8401 agctcctcct tttccagatt ggctatttat ccttataatt tctttgtatt ctcttccaat
     8461 atcatgaggg tctattggta aatatggaac ttcccagtaa tcattatctc tgtttttcat
     8521 tgctatcatg ccttttctaa tagcatcttg atgtgaacct gaaaaagcac aatgtacaag
     8581 attacctgca tatggatgtc tatcatgtac cataagtttt gtacagtctt catatatacc
     8641 tattatctta tctatattgc taaaatctag ctctgggtct attccttgtg tataaagatt
     8701 aagtcccaca tttactatat ccatattccc agttctttca ccatttccaa atagtgttcc
     8761 ttcaagtcta tcggcaccag ccaataatcc aagttcactt gcagccgtac aagttcctct
     8821 atcattgtga gtatgcaaac ttagtataat agaatcccta caacttatat ttttacaaaa
     8881 ccattctatt tggtctgcat atacgtttgg tgttgccatt tccactgttg atggaagatt
     8941 tattattact tttttttgag gagttggttt ccaaacatct atcactgcct cacatatttc
     9001 aagtgcataa tccatctctg ttcctgtaaa actctctggt gaatattcaa aagtaaattc
     9061 tgtttctgga tattttttta cttcttcatt aaataatttt gctccttcaa ctgctatatc
     9121 tattatttct tgtttactca tattaaaaac cacatctctt tgaagaacag atgtagagtt
     9181 atataaatgt ataattgctt ttttacatcc ccttattgat tcaaatgttt tttctataag
     9241 atgagcccta gattgtgtaa gtacttgtat agttacatca tctggaatca tattctcatc
     9301 tattaatttt cttaaaaatg tatactctgt atttgatgct gatggaaatc ctacctctat
     9361 ttctttaaat cctgtatcaa taagcatttt gaacattctg actttttcat ttacactcat
     9421 aggtgttggc agtgactgat ttccgtctct taaatcgaca ctacaccaaa ttggagcctt
     9481 aaatatttca ttatctggcc atttacgatt tactatttgc attttatcat attttttata
     9541 ctttccacac ttcatgttat ttcccccttt ttttgttttt gtattcagta tacctcatat
     9601 cgacgcaatc ctgcacaatt cataaggata aatgtcaatt ttatcattag aataaaaaaa
     9661 acctcatctc taataaaatt agagacgagg ttttgctcgc ggtaccactc tatttagctt
     9721 tctaaaaagc tcccttagta atagccaata caataattat aatgactata tttcctataa
     9781 cgtggaatta ctccgattat tctaagattt aaatcttaga aaacagctcc tagacaagtt
     9841 cagaactatc aactactaaa tcacaccatc ctttagctct ctgaaagttt cattgttctt
     9901 actacttcta ttcatcactt ttttaatcat ttaaattttc taaatatttt gatttaatag
     9961 ttattatata cacattaaaa tgtattgtca acacaaaaaa taattatctc aattttttat
    10021 ttttgataag caattctata aaacatgcta actattgaaa acagtatgat attatgcctt
    10081 aaactttttc atcttttcat ctatgatatt caataatttt ttttcatctt ccaaactcaa
    10141 tgcccttaat ctaattttat aattagattc gtcagtgtat atatttaata tattttttac
    10201 tacttctatt ttttctatta tcttataatt aaacttttga ctttgtttag attttatact
    10261 gaatatacat tgattattta ttaatattga atgattatcg agagtaatat atacttttcc
    10321 ctcatattta tttgaaaaat catgaccttg tttaaataga aaaactgctg ttattataca
    10381 taaaatatat aatattgtcg ttgaatatat aaaaattgta gctaaaatta taagtgctaa
    10441 aaccatatat atagtcattc cttttttaaa ataaccattg tcataataat tcatatagat
    10501 aaacttattt ttactcaaat tttttcactc ccaagattct aaaaatttat atcaaaaaat
    10561 tctataattt catcttcatg ataatttatg actaattcat ctggaaaatc tactgcttct
    10621 aaaacttttt ctgcattttc aaattctcct atagaataac atatatgaga gtcagtacca
    10681 agaattactc ttgcaccata tgttttacac aatgtcaaca tatgacttac attttcccaa
    10741 gtacccgttc tatgagagtt tgaacttaat gatgaattat ttatttctaa aaggatattt
    10801 ttatctttag ctttttttac tataggttca taatctaaag gatacctact gtcatctgga
    10861 tgtccaatta tttttacctt aggtttatcc ataactttta aaatagcatt tgtattttct
    10921 tcttttgtac cagaagctat acaaggtcta tgcaaacttg cgattatata atccaaatgt
    10981 tgcaacatat cttcttgaac atctaaatta ccattataat ccattatatt tccttctatt
    11041 cctctaagaa tctttacgcc ttgcacttca cgtggaagca aatgcaaatt ataaaaatag
    11101 aatggatgtg gtcctcctgg catattaggt ccatggtctg atattcctaa gtattttata
    11161 ttcttcttac tcgcctcttc gacattttcc ttgaaagtac tatgtgcatg tccacttatt
    11221 agagtgtgac aatgtacatc cataatagac tttttcatta aatttcctcc taatatttta
    11281 cataaattcg tgaaaatagc taaagggcta tttcaaaact aaagctgtca taatagacag
    11341 tgtaacgttt taaaacagcc ccattttcaa ttattttaca cttctaaatc tttaaattta
    11401 tctttaaata attctccaat acttcctaaa gattcctcta cttctaggta ttctgttata
    11461 tcctcttctg gctctctagt tgcttctttt attgaaacca acattctttt agtctttggt
    11521 ttaaaatcaa gaatcttaac tttaacctca tctccaatat taacaacatt agtaacttta
    11581 gccactctag tatcactcaa ttcagatatt ggtaaatacg catctacacc ttctttaatt
    11641 tgaacaaaag cccctttttc tattattcta agaactttag cattgacaac atcatcaatt
    11701 tgtacttctt ttgctattaa atcccaaggg tcattattta tgtcttttaa tgccaaggca
    11761 atttttttag tctctttatt tacatccaac acataaactt ctacttcttg tccctctgaa
    11821 acaacatctt caacactttc aactcttgtc caagctaaat tatttatatg tgctaatcct
    11881 tcaatcccac caatatccac aaatgcacca taaggcatta tctttgtcac cttaccatct
    11941 cttttttgtc caacttcaag agaattaaat aagtcttctt tagctgcttt gatacgagct
    12001 ttttcttctt ctctttcttg cttaattctt gctctttcag cttctttttc tgccttaatt
    12061 tgagctcttc tttcttctct ctctttattt atacgctctt gtaatacatc tctatgagat
    12121 gctacaagtc tattttttct aacatccaat tcttttatat aaacttctaa gttaagacca
    12181 acaaatttag atgtatctgt aataaatttt gtatcaagtt gagaaattgg tataaaaaat
    12241 gtatatgtat tgtaattagc aaatacacct ttatctatat tcttttcaac atttacagtt
    12301 attattctat gttcatcata agccttttgt aactcggcaa aatcatttct tttgtcaagt
    12361 tgtaatttag ataaagttat tgtaccatct ttttgagata cttttgttat tacagcagtt
    12421 atttcatcac ctatgtggta tatatcttct atatattgat ttttttctat gttcaattca
    12481 cttataggta ttattccatc aaacccatag tctaatccta attgtacttc atcatttctc
    12541 actgcagtta tttttccagt tgtcaattct ccaactttca ctttgctgaa tacttgttct
    12601 tgctggtcaa gtaattcttg cattgttaaa tcattttcca ttataaacat cctttcctct
    12661 tattgattat catatctgta aatattatac cattaatatt gcaaatactt acatttttct
    12721 tactataatt ttt
//
LOCUS       DAEMHW010000112        12538 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEMHW010000112 DAEMHW010000000
VERSION     DAEMHW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.112"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..2223
                     /locus_tag="KPK67_002514"
     CDS             <1..2223
                     /locus_tag="KPK67_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HBG2459465.1"
                     /translation="MPERPDRRTREVQAESVAYAVCQHYGLDTSDYSFGYIAGWSSGK
                     ELAELKGSLETIRSTAANLIDSIDGHFAEIQKAQDKEQTTEQAQPAQEADKQPEAEAV
                     APEFPEETAPVQEKEAQTEPEADTGASSEAPQTTQPEQTAPAAPYYTINEAAARRAKD
                     ANSFSDYKQGSATAEYRHYVDGAVQLAERQKRRVDPMYHEKIDSLLDTYARKLAANMN
                     KGYEIDARVPSILIAGGSNFPTRKKEKQNAARGSNYREWQDIQGLLDKIRSTGMGGIS
                     ADDPQAVQKLEKKLESLEKSQETMKAVNAYYRKHKSLDGCPHLPPEELEKLKADMASS
                     WHLKDKPFATWALSNNSAEIRRVKDRIKSLSQQKEIGFVGWEFDGGKVEANTEANRLQ
                     IFFEDKPDEATREALKSNGFRWSPKAVAWQRQLTSNAYYAADYVKAIAPLTGEKPTEL
                     QRAHIRAQKVAAQKQPTQEQPEQEAPQDKNAFSIYQIKGGDETRDLRFEPYDRLTATG
                     HRVDPKNYVLVYSAELTPGTSLEDIYTRFNIDHPKDFKGHSLSVSDVVVLHQNGQDTA
                     HYVDSFGYKEVPEFFKEQEKALIPDDLETGETIKTPRGTFYVTAMSREQMEAAGYGLH
                     HQSDDGKYLIMGNGTRAFAIAAEQPENYLKAAEQTTEQNYNMIDGQINNTPTAAELEE
                     KAKAGGQISLAEYAEALKAEKKQAAPEKKPSIRAQLRADKENTAQKKAAKSKNHELEV
                     "
     gene            2226..2432
                     /locus_tag="KPK67_002515"
     CDS             2226..2432
                     /locus_tag="KPK67_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBG2459466.1"
                     /translation="MIRLTVEETNLLSIYHEGNKAQLIENMTAALPFMDEDMRPFAER
                     TLQKISPLTENEYAELAIFAADEV"
     gene            2429..2794
                     /locus_tag="KPK67_002516"
     CDS             2429..2794
                     /locus_tag="KPK67_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBG2459467.1"
                     /translation="MTGVKRLTPPQSRKVNSLVKKECCNCERGHCILLDDGEECICPQ
                     LISYSLLCKWFQTAVLPLDRLLYAELFKTEDKKRCSECGAFFASTSNNVKYCPDCRKR
                     ITRRQAAERMRKRRALVTQ"
     gene            complement(2970..3419)
                     /locus_tag="KPK67_002517"
     CDS             complement(2970..3419)
                     /locus_tag="KPK67_002517"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459468.1"
                     /translation="MKYGFDYIDGRDVPQTVIEDDFTIQGKHNGTVHVENGTLTIQGE
                     LHGTLDVQSGAKAIIIGKQHGTVSVANNAEIVIFGELNGTTNVSVYGTVVVEAHGKLA
                     GTLSNNGTVIVRGVFGGAQSGHGELILEENGYIKQPVIKNGMKYYEW"
     gene            complement(3489..4835)
                     /locus_tag="KPK67_002518"
     CDS             complement(3489..4835)
                     /locus_tag="KPK67_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBG2459469.1"
                     /translation="MAVTKIKPIKSTLSKALDYIQNPDKTDGKMLVSSFGCSYETADI
                     EFGFTLAQAIEKGNNLAHHLIQSFEPGEVDYEKAHEIGKQLADAVTKGQHEYVLTTHI
                     DKGHIHNHIIFCAVNFVDYHKYNSNKRSYYGIRNVSDKLCRENGLSVVVPGKGSKGKS
                     YAEYQAEKVGTSWKGKLKIAVDTLIPQVASFEELLQRLQAAGYEIKPGKYISCRAHGQ
                     ERFTRLKTLGADYTEEAIKERIEGKRTKAAKAPKEQRGVSLLIDIENSIKAQESRGYE
                     QWAKIHNLKLAAKTMNFLTEHKIEQYADLISRIEEINTENVKTADALKSVEKRLADMA
                     LLMKHVTTYQKTKPVYEAYRRAKDKDAYRAKQESSLILHEAAIKALKASGITKLLNLA
                     AMQEEYGKLQAQKEAFYVDYGKLKKQVKEYDVIKQNIDSILRQGKEPERDKETERG"
     gene            complement(4796..5125)
                     /gene="mobC"
                     /locus_tag="KPK67_002519"
     CDS             complement(4796..5125)
                     /gene="mobC"
                     /locus_tag="KPK67_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBG2459470.1"
                     /translation="MNGRKRKVQIKFYVTEEERVLIEQKMKLVPTRNMAAYLRKIAID
                     GYIIQTDHSDIKAMTAEIQKIGVNINQIAKRVNATGSVYQEDIEEIKGVLAEIWRLQR
                     LSLLKAR"
     gene            complement(5369..5728)
                     /locus_tag="KPK67_002520"
     CDS             complement(5369..5728)
                     /locus_tag="KPK67_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2459471.1"
                     /translation="MKQSKEKFDFKAFGQAIKAARKANGISRNQLADTLNIAPRYIAS
                     IENSGQHPSLQILYELVTLLDVSVDQFFFPEREQEKSTRRRQLDTMLDTMSETDLKIM
                     SATAKGIEEANNGRTGE"
     gene            5904..6098
                     /locus_tag="KPK67_002521"
     CDS             5904..6098
                     /locus_tag="KPK67_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG2459472.1"
                     /translation="MEYISCMEAAENWGISERRVQKLCEENRIPGATKFSRIWLIPKK
                     AEKPIDRRKQHAADTTNSNR"
     gene            6067..6756
                     /locus_tag="KPK67_002522"
     CDS             6067..6756
                     /locus_tag="KPK67_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2459473.1"
                     /translation="MQQILQILIVEDDILLSDMLKSLLSQNGYRVNTAYSGSEALLIL
                     EKETFDLILLDLMLPGISGEEVLTTIRKKQTTPIIGVSAKTDIDSRIDLIKNGADDYI
                     TKPFDNKELLVRMEAILRRCHRSSNNSLLKFKDLMLNPATMEVTIKDIPLVLTRYEYL
                     ILQLLMTAPNKVFTKNNIFESVWNETFCGDDNAINVHISNLRKKFAKINPNEQYIQTV
                     WGLGFKMQSNP"
     gene            6788..7714
                     /locus_tag="KPK67_002523"
     CDS             6788..7714
                     /locus_tag="KPK67_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459474.1"
                     /translation="MHNTYTIIERGGSVGLVLEVKNLSYVFPNGVGIIGIDFQIDYND
                     IYYLYGSHNSGKSLILQSILGIISPQKGSIKIFENPDFLQEKRRIGYVPQSPYYIDRM
                     TITDMMNYFSLSFGVLEKNFADILNLNLKEKKPVRHLSSFIQRKLNLWIALLGNPDLV
                     FIDDPFGGLDTQESEQLISIISYLNKNNNITFLLTGHNYELASRIATKYGILKNGRFL
                     IELTPEKLDSECKRCIKIRTPQLLSAIPLLEKEFPQYEVLEDDLIRVFCPLSHSAKIN
                     TLLVSAGIEVSEIWIAGTMPQEYLLKLTEGGI"
     gene            7718..8494
                     /locus_tag="KPK67_002524"
     CDS             7718..8494
                     /locus_tag="KPK67_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2459475.1"
                     /translation="MIKMIQGELFRIRKNRLFKICLIVSTFFVFALALTFHYNTIKHS
                     VAAVPKASLNDYTQFLFSDYSLILPITVFLGYYFTENSRTGTCSIFLSKGITRTSIFF
                     GKLISSWIATVLYMLFNFLMAYIFILVMWVKQSYIDCSFGLIGIYLFLQILCFIGYTS
                     FIWLLSILFRYRSLVITISIFLLGTLYFYLTKISSALDLSYSLYRYWIAGLSHAMEIR
                     LVLPQLPLILLTITGYVFFPTLCSFVIYKKIDLRKHERDK"
     gene            8496..9392
                     /locus_tag="KPK67_002525"
     CDS             8496..9392
                     /locus_tag="KPK67_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2459476.1"
                     /translation="MSIFCVFLVVTSLILLLKVIIQKQQITKIKNQIDFLCSRDTQAE
                     ITLEKLDKQTEELAISINHLLEKYRLTGIKIERNDSLFRDTITSLSHDLRTPLATANG
                     YIQLLLEQPLSAEQKEYVKIANERVSAVKILLDQLFEFARIEANELKLNKQLVDANSV
                     LRDALATYYNDFEIKAITPTIDIPDSPAMIWADKDALFRVFSNIIYNALIHGDSVYTI
                     HATVERKCYYISISNHSNTICKEDIPHLFDRFYTTDSSRTKKTTGLGLAIARKLITQM
                     GGEISAHLHNEIFEICISFTIL"
     gene            9502..9678
                     /locus_tag="KPK67_002526"
     CDS             9502..9678
                     /locus_tag="KPK67_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="receptor"
                     /protein_id="HBG2459477.1"
                     /translation="MLSKISLIISIVALAVCFIAAISGILSDGMKGLQKKGKLLGVTF
                     CIYAITFLTFIISQ"
     gene            9691..10380
                     /locus_tag="KPK67_002527"
     CDS             9691..10380
                     /locus_tag="KPK67_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2459478.1"
                     /translation="MLQIKNVNLCVGSNKLLHNINLSLEQGKIYGLLGANGAGKTTLF
                     KSMLGLTAYTGEILSDKNPAKSSEFGSLIEYPAFYPKLSVTENLKLHASYIGLTNPDL
                     ENSLKQVNLWEARNKLFSQLSLGMRQRLGIARTFLGNAKYLLLDEPTNGLDPMGIKDI
                     RILLRDKLKTPKHCIIVSSHNLTEIAAIADILIFIRNGQIIEMIENKYDEKELEVLYE
                     NLMTSITTEEV"
     gene            10380..11135
                     /locus_tag="KPK67_002528"
     CDS             10380..11135
                     /locus_tag="KPK67_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2459479.1"
                     /translation="MKVLIKNEFTKLKREWFVLFLLLLSWLPVLTGGVGAVFNNSTKT
                     VSDLFFFMNNQFSMFFPMVIVILTGSLFYQEYKNKTYINWITYGYSKQQLFSAKIIVG
                     FVIEIVFAASLICLFHILILMLKIAGKVSVDIQEILSLTVGFALEAIIVILITTCIAA
                     IIINISRNIIVTSVIGVIYGFVSCFFIGSEFGYVIPGSFAYRLSMYFSDSSTYYELAK
                     QATIGGCISSIISFGLMLFMGIFIFTRKKKIES"
     gene            11598..12038
                     /locus_tag="KPK67_002529"
     CDS             11598..12038
                     /locus_tag="KPK67_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2459480.1"
                     /translation="MEPNRREFQKQCVFQSFCKRVLHNEACNAHDEIRRRRKREVSFC
                     DLALHEERQLYTTDKYFQAETAEPTYQMAGKEITPKLLLEAIRTLPEEKRTAILLYYF
                     EGMTDVEIGKMFNTSRSTIQYRRTSSFELLKKYLEEHADEWNEW"
     gene            12019..12276
                     /locus_tag="KPK67_002530"
     CDS             12019..12276
                     /locus_tag="KPK67_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2459481.1"
                     /translation="MNGTNGNQNDYPENALVPYPVILAASKGDPDAMKMVLQHFSGYI
                     ASLSMRKLYDERGNVYYGVDEDIRERLQARLMRAILTFRAE"
BASE COUNT         3754 a         2619 c         2565 g         3600 t
ORIGIN      
        1 atgccggaac gccccgaccg ccgcacccgc gaggtacagg cggaaagcgt cgcctatgct
       61 gtctgccaac attacgggct tgatacgtcg gactattctt ttggatatat cgccgggtgg
      121 agcagcggaa aagaacttgc agaactgaaa ggctctcttg aaacaatccg cagcaccgcc
      181 gcaaatctga ttgattccat agacggacat tttgcagaaa tccaaaaggc acaggataag
      241 gagcagacca ccgaacaggc gcagcccgca caggaagccg acaaacagcc cgaagcagaa
      301 gccgtcgcgc cggagtttcc cgaagaaaca gccccggtac aggaaaaaga agcacagaca
      361 gagccggaag ctgatacggg cgcaagcagc gaagcaccgc agaccacaca gccggaacag
      421 actgcccccg ccgcccctta ttacacaatc aacgaagctg ccgcccgccg cgcaaaggac
      481 gcaaacagct tttctgatta taagcaaggc agcgcaacgg ctgaatacag gcactatgta
      541 gatggagccg tacaacttgc agaacgacag aaacgccggg tagacccgat gtaccatgag
      601 aaaattgaca gcctgcttga cacctacgcc cgaaaactgg cggcgaacat gaacaaaggc
      661 tatgagattg acgcgcgtgt tccctctatc ctgattgcgg gcggctctaa cttccccaca
      721 aggaagaaag aaaagcagaa cgcagcccgc ggcagcaact accgggaatg gcaggacata
      781 caagggcttc ttgataaaat ccgcagtacc ggcatgggcg gtatcagcgc agacgaccca
      841 caggcagtac agaagttgga aaagaaactg gaaagccttg aaaaatcaca ggaaaccatg
      901 aaagccgtaa acgcctatta ccgcaagcat aagtcccttg acggctgccc gcatttaccg
      961 ccggaggaac tggaaaaact gaaagcagac atggcgagca gttggcattt gaaggacaag
     1021 ccctttgcga cttgggcgtt atccaacaac agcgcggaaa tccggcgcgt gaaagaccgt
     1081 atcaaatccc tttcacagca aaaggaaatc ggttttgtgg gttgggaatt tgacggcggc
     1141 aaggtggaag caaacaccga agcgaaccgc ctgcaaatct tctttgagga taagccggac
     1201 gaagccacgc gggaagccct aaaaagtaat ggtttccgtt ggtcgccaaa agccgtggca
     1261 tggcagcggc agcttaccag taacgcctat tatgcggctg attatgtgaa agccattgca
     1321 cctcttaccg gggaaaagcc gaccgagtta cagcgggcgc atatccgggc gcaaaaggta
     1381 gctgcacaga aacaacccac acaggagcag ccggaacagg aagccccaca ggacaaaaac
     1441 gccttttcca tttatcagat aaaaggcggg gacgaaacaa gggacttgcg ctttgaacct
     1501 tacgaccgtc tgactgccac aggacaccgg gtagacccga aaaactatgt gcttgtctat
     1561 tccgcagagc ttacgccggg gacttccctt gaagatattt acacccgctt caatatcgac
     1621 cacccgaaag attttaaggg acacagcctt tccgtttccg acgtggtggt gcttcatcag
     1681 aacgggcagg acaccgccca ctatgtagac agcttcgggt ataaggaagt gccggagttt
     1741 ttcaaggagc aggaaaaagc actcattccc gacgacttgg aaacgggcga aacaatcaaa
     1801 acaccgaggg ggactttcta tgtaaccgcc atgagccgcg agcaaatgga agctgccgga
     1861 tatggattgc accaccagtc cgacgacgga aaatatctga ttatggggaa cggaacgcgg
     1921 gcgtttgcca ttgccgcaga acagccggaa aactatctga aagctgccga gcagaccacc
     1981 gaacaaaact acaacatgat tgacggacag ataaacaaca ccccgaccgc tgcggaactg
     2041 gaagaaaagg caaaagccgg aggacagatt tctcttgcag aatacgccga agccctcaaa
     2101 gcagaaaaga aacaggcagc cccggagaaa aagccctcta tccgggcgca gcttagagca
     2161 gacaaagaaa ataccgcaca gaaaaaagca gcaaaatcaa aaaatcacga attggaggta
     2221 tgagcatgat tagactgacc gttgaagaaa cgaacctgtt gagtatatac catgagggaa
     2281 acaaggcaca gcttattgaa aatatgacgg ctgcgctgcc gttcatggac gaggatatgc
     2341 gcccctttgc agagcgcacc ttacagaaaa tctccccact aacagaaaac gagtatgcgg
     2401 agcttgccat tttcgccgct gatgaggtat gaccggggtt aagcggctta cgccgcccca
     2461 aagccggaaa gtcaacagcc ttgtaaaaaa ggagtgctgc aactgcgagc gcggacactg
     2521 tatcttactg gacgacgggg aggaatgtat ctgcccgcag cttatttcct attcactttt
     2581 atgcaagtgg tttcagactg ccgtacttcc ccttgacaga ctgctctatg cagaactttt
     2641 caagaccgaa gataaaaagc gttgtagtga gtgcggggcg ttctttgctt ccacctctaa
     2701 caacgtcaag tattgcccgg attgccggaa acgtattacc cgcagacagg cagcggaacg
     2761 catgagaaaa cggcgtgcgc ttgttacgca gtaggggtta aaaaagcctt attttatgcg
     2821 gcttgcagag tgtgaaaatt acttaggcga tacagaatac cttttccctt aaaatagcat
     2881 tctactgcgt aacataccaa aatcagcacc cacaaaaaga cagggcgcgg aggtcattac
     2941 tgactttccg cgtcctgttc tttttttgct taccactcgt agtatttcat tccgtttttg
     3001 ataactggct gtttgatata tccgttttct tcaagaatca attcaccgtg tccagattgc
     3061 gcgccaccaa aaacgcccct aacaattaca gtgccattat tactaagtgt tcctgctaat
     3121 ttaccatgcg cttcaacaac cacagtacca taaacactaa cattagttgt accgttcaat
     3181 tcgccaaaga ttactatttc cgcattgtta gctactgaaa cagtaccatg ctgtttgcca
     3241 ataataattg cttttgcacc actttgcaca tctaacgtac catgtaattc accttgaatt
     3301 gttagtgtac cattttcaac gtggactgtt ccgttatgtt tgccttgaat agtaaaatca
     3361 tcttctataa ccgtctgcgg tacatctctg ccatcaatgt aatcaaaacc atatttcata
     3421 aaaatacccc cttgcgttta tgataaatta taccatacct ttatgaataa atccaccacc
     3481 aaagagagct atccgcgctc tgtttccttg tcccgttccg gctctttgcc ttgccgtaaa
     3541 atgctgtcta tgttctgctt gataacgtcg tattccttga cctgtttttt cagcttacca
     3601 taatcaacgt aaaaggcttc tttctgcgct tggagcttcc cgtattcttc ctgcatggcg
     3661 gcaaggttca ggagtttggt aatgcccgac gctttcagtg cctttattgc cgcttcatgg
     3721 agtatcagac tgctttcctg ctttgcccgg tatgcgtcct tatcctttgc cctgcggtat
     3781 gcttcataaa caggttttgt cttttggtag gtggtaacgt gcttcatcag caacgccata
     3841 tctgcaagac gcttttccac gctctttaat gcgtctgctg tctttacatt ttccgtattt
     3901 atttcctcaa tccggctgat taaatctgcg tactgctcaa tcttatgttc tgttaagaaa
     3961 ttcatagtct ttgcagccag tttcagatta tggattttcg cccactgttc atagccccgg
     4021 ctctcttgtg ccttgatact gttttcaatg tcgataagca gcgatacgcc gcgctgctct
     4081 ttgggagctt tcgccgcctt tgtgcgtttg ccctctatcc gttctttgat tgcttcctct
     4141 gtataatctg caccgagagt ttttaagcgg gtaaatcgtt cctgtccgtg agcgcggcag
     4201 gagatatatt ttcccggttt tatctcatag cccgctgcct gtaaccgctg caataattct
     4261 tcaaaacttg ccacttgggg gataagcgta tcaacggcaa ttttcagctt gcctttccaa
     4321 cttgtaccga ccttttccgc ttgatactcc gcatagcttt ttcccttgct gcctttgccc
     4381 ggaacgacga cggacagccc gttttcccgg cacagcttat cgctcacgtt ccgtatgccg
     4441 tagtagctgc gcttattgga attgtactta tggtagtcaa caaaattgac cgcacaaaaa
     4501 atgatatgat tatggatatg ccctttgtct atgtgggtag ttaagacgta ttcatgctgt
     4561 ccctttgtta ccgcgtcggc aagctgtttt ccgatttcgt gggctttctc atagtctact
     4621 tctcccggct caaaggactg tatcaaatgg tgggcgagat tgttcccttt ctctatggct
     4681 tgtgcaagcg taaatccgaa ctcaatgtct gctgtttcat agctgcaccc aaaagaggac
     4741 acaagcattt tcccgtctgt tttatccggg ttttggatat agtcaagggc tttactcaac
     4801 gtgcttttaa taggcttaat ctttgtaacc gccatatctc tgcaagcacc ccctttattt
     4861 cctctatgtc ctcttggtag acgctgcctg tcgcattgac acgctttgca atctgattga
     4921 tgttgacccc gattttctgt atctctgctg tcatggcttt tatgtcgcta tggtctgtct
     4981 gaatgatata cccgtcaatg gcaatcttgc gaagatacgc cgccatgttc cgggtgggga
     5041 cgagcttcat tttctgctca atcagtaccc tttcttcctc tgttacatag aatttgattt
     5101 gtacttttct tttgcgtccg ttcatgcaat cgctcctttc cattccgagg gtttgggaca
     5161 cttcccaaca agcaattttc aaccaacgca ccgcaggacg ggagggcgaa aatatggaaa
     5221 agggtactct tttcctatgc ttgctacgaa agttttttcc tcacttatca ctacaacaaa
     5281 actcccagta tgccaaaaaa gagcaaaaga aaaacgccgc aatcctgcga cgcttcaatc
     5341 actttatgca gacaaaaacg agggaacttt attcccccgt cctgccgttg ttggcttcct
     5401 ctatgccttt tgcagtagca gacatgattt ttaagtccgt ttcgctcata gtgtcaagca
     5461 tggtatcgag ctgacggcgg cgtgtggact tctcctgttc ccgttccggg aaaaagaact
     5521 ggtctactga tacgtcaagc aaagtcacaa gctcatacaa aatctgtaaa cttgggtgct
     5581 gcccgctgtt ctcaatggac gcgatatatc ggggcgcaat attcagcgtg tcggctaact
     5641 gattgcgtga tattccattt gcttttcttg ctgctttaat ggcttgtccg aaagccttaa
     5701 aatcaaattt ctctttactc tgcttcataa ttatttcacc cagttacatt ttactgttcc
     5761 tcttttcttt gggatatgat tacacatagc gtagtattga ctaaaataga gcatattatt
     5821 tatgcgccac ttcttgtatt tgttcggttg aacgtatata atcatattgg cgaagttaat
     5881 ttctgatgaa agggtgtcaa aatatggagt atatatcttg catggaagcc gcagaaaatt
     5941 ggggaatttc tgaaagacga gtacaaaagt tgtgtgaaga aaacagaata cccggagcga
     6001 ctaaattcag ccgtatatgg ctgataccaa aaaaagcaga aaaaccgatt gacaggagga
     6061 aacagcatgc agcagatact acaaattcta atcgttgaag atgatatttt actaagtgat
     6121 atgctaaaaa gtctattatc tcaaaacgga tatagagtta atacagctta ttcggggagt
     6181 gaagcacttt taatccttga aaaagaaact tttgatttaa ttttacttga tttaatgctg
     6241 ccgggaatat ctggcgaaga agtattgaca acaataagaa aaaaacaaac tacgccgatt
     6301 attggtgtgt ctgcaaaaac ggatattgac agtagaattg atttgataaa aaacggtgct
     6361 gatgattata ttacgaagcc ttttgataat aaagaactgt tagtgcgaat ggaagctatt
     6421 ttacgtcgct gtcatagaag cagcaataac tccctattaa aatttaagga cttaatgctt
     6481 aatccggcaa caatggaagt gacaataaaa gatattcccc ttgttctcac aagatatgaa
     6541 tatttaattt tacagttatt gatgactgcc cctaataagg tctttaccaa aaataatatc
     6601 tttgaaagtg tatggaatga aactttttgc ggagatgata atgcaatcaa tgtccacata
     6661 agcaatcttc ggaaaaaatt tgctaagata aatccgaatg agcagtatat acagacagta
     6721 tggggattgg gctttaaaat gcagagtaat ccttagaaaa tgcttataaa taaagctata
     6781 atctttaatt cacaatacat atacgataat agaaagaggt ggaagtgtgg ggcttgtttt
     6841 agaagtaaaa aatttatcct atgttttccc aaatggcgtt ggtattattg gcatagactt
     6901 tcaaatagat tataacgata tttattattt atatggaagt cataactccg gcaaaagttt
     6961 aatattacaa tcaatacttg gtataatttc tccacaaaag ggaagcatta aaatttttga
     7021 aaatcccgat tttttacaag agaaaaggcg tattggatat gtaccacaaa gtccatatta
     7081 cattgacaga atgactataa cagatatgat gaattatttt tctttatcat ttggggtatt
     7141 agaaaaaaac tttgcagata tattaaacct taatctgaaa gaaaagaaac ctgttcgtca
     7201 tttatcttcc tttatccaac gaaaactaaa tttgtggatt gctcttttag gaaatcctga
     7261 tttggttttt atagacgacc catttggagg actcgatact caagaaagtg aacaacttat
     7321 ttcaatcata tcttatctga ataagaataa taatatcact tttttgttga caggacataa
     7381 ttatgagctt gcttcccgaa ttgcaacaaa atatggaatt cttaaaaatg ggcgtttctt
     7441 aatagaactc actccagaaa aattagacag tgagtgcaaa cgctgtatca aaataagaac
     7501 gccacaatta ctttctgcaa ttcctttatt agaaaaagag ttcccgcaat atgaggtttt
     7561 agaagatgat ttaatcagag tattttgccc cttatctcat tctgctaaga taaatacatt
     7621 attagtatcc gccggaatag aggtatcaga aatttggatt gctggaacta tgccacaaga
     7681 atatcttcta aaattaacgg aaggaggaat atagcctatg attaaaatga tacaaggaga
     7741 gctttttcgt attcgcaaaa atcgtttatt taagatatgt ttgatagtat ccacattttt
     7801 tgtttttgca cttgcattga cttttcatta caatacaata aagcactccg ttgcggctgt
     7861 tccgaaagcg tccttaaacg attatacgca atttttgttt tcagattata gtttaatact
     7921 tcctattacg gtattcttgg gctactactt cacagaaaac agccggactg gtacttgttc
     7981 gattttcttg tcaaaaggaa ttacaagaac atcaattttt tttggtaaac taatttcttc
     8041 atggattgcc actgtcctgt atatgctgtt taattttctt atggcatata tttttatctt
     8101 agttatgtgg gtaaaacaat cctacattga ttgctcgttt gggctaatcg gtatctacct
     8161 gtttttacag attttatgct ttataggcta tacttctttt atttggctac taagtatctt
     8221 atttcgttat cgtagccttg ttatcacaat ttccattttt ttattgggga cattatactt
     8281 ctatcttaca aaaataagtt cagccttaga cttatcctat tcactatatc gttattggat
     8341 agcagggtta tctcatgcaa tggaaatcag attagtactg ccacaattac cgctcatatt
     8401 gcttaccatt accggatatg tgtttttccc tacattatgt tcattcgtta tttacaaaaa
     8461 aatagatttg cgcaagcacg aaagggataa ataatatgag tatattttgt gtttttcttg
     8521 tagttacatc tttgatatta ctattgaaag taattattca aaagcaacaa ataactaaaa
     8581 tcaaaaatca aatagacttt ttatgttcca gagatacaca agcagaaata accttagaaa
     8641 aactggataa acagacagaa gaacttgcta ttagtatcaa tcatcttttg gaaaaatata
     8701 gattgaccgg gataaaaatt gaacgaaatg acagtttatt tcgtgataca atcacaagcc
     8761 tttcccacga tttgcgtacc ccgctcgcaa ctgcgaatgg ctatatacaa ctacttttag
     8821 aacaacctct atccgctgaa cagaaagaat atgtaaaaat agcaaatgag cgagtttcgg
     8881 cagtaaagat attgcttgac cagctttttg aatttgcgag aattgaagcg aatgaactaa
     8941 aactaaataa acagttagta gatgccaaca gtgttttaag agatgcctta gcaacatact
     9001 ataatgactt tgaaataaaa gctattaccc ctactattga tattcccgat agtccggcta
     9061 tgatttgggc agacaaagac gcattattta gggttttctc aaatatcata tataacgctt
     9121 taatacatgg cgatagtgtt tatacaatcc atgcaacagt tgaaagaaag tgttattata
     9181 tcagtatttc caaccattca aatacaatct gtaaagagga tatacctcat ctgtttgacc
     9241 gtttctatac aacagacagt tctcgtacaa aaaaaacaac gggactgggg ttagcaatcg
     9301 cgcgtaaact gattacacaa atgggtggag aaatatctgc acatttacac aatgaaatat
     9361 ttgaaatatg tatttccttt acaattttat aaaaacaggg tggctatggc taccctgttt
     9421 ttatctttag acttttctta gacttttcct aaaaaaatca taactctatt tgttactata
     9481 cattacaagg gaggtggtca aatgttatct aaaatttcct taatcatttc gattgttgca
     9541 cttgctgtat gttttattgc agcaatcagt ggtattttaa gcgacggtat gaaaggcttg
     9601 caaaaaaaag gaaaactact tggtgttaca ttttgtattt atgcaataac ttttttgact
     9661 tttataatct cacaataatg gaggtctaca atgttacaaa ttaaaaatgt aaatctttgt
     9721 gtcggaagca acaagctact tcacaatatc aatctttctt tagaacaagg aaagatatat
     9781 ggactacttg gtgcaaatgg cgcaggcaaa accacattgt ttaagtctat gttaggactg
     9841 actgcttata caggcgaaat tctttcagat aaaaatcccg ctaaaagtag tgagtttgga
     9901 agtttaattg aatatcctgc attttaccca aaactttccg tcacggaaaa tctgaaactt
     9961 catgcaagtt atattgggct tacaaatccc gacttagaaa attctttgaa acaagtcaat
    10021 ttatgggaag caagaaataa gctcttttca cagctttctc ttggcatgag acagcgactt
    10081 gggattgccc gcactttttt aggaaatgcg aaatacttac tattagatga gccaacaaat
    10141 gggcttgacc ctatggggat aaaggacatt cggatattat tacgtgataa attaaaaacc
    10201 ccaaagcact gtattattgt atcaagccat aatctcacag aaattgcggc aatcgcagat
    10261 atacttatct ttatccgtaa tgggcagatt atagaaatga ttgaaaacaa atatgatgag
    10321 aaagaattgg aagttcttta tgaaaatctt atgacttcca taactacgga ggaagtataa
    10381 tgaaagtttt aataaagaac gaatttacta aattgaaacg tgaatggttt gtattatttt
    10441 tacttctcct ttcatggcta cctgttttaa ctggcggagt gggggctgtc tttaacaata
    10501 gtacaaaaac cgtaagtgat ttatttttct ttatgaataa tcaattttcc atgtttttcc
    10561 ctatggtaat tgttattttg acaggttcgc ttttttatca agaatataaa aataaaacct
    10621 atataaactg gattacttac ggatattcaa aacaacagtt attcagtgca aaaattatcg
    10681 tcggatttgt tattgagatt gtatttgccg cctcgctaat atgcctgttt catattttga
    10741 ttttaatgct gaaaatcgca ggaaaagttt ctgttgatat tcaagaaatt ttatccttaa
    10801 ccgtaggatt tgcattagaa gctataattg ttattctgat tacaacttgc atagcagcaa
    10861 ttatcatcaa tataagcaga aatatcattg taacaagtgt tataggcgta atttatggat
    10921 ttgtgtcctg cttctttatt gggagtgaat ttggatatgt tattccgggg agttttgcct
    10981 atcgtttatc aatgtatttt tcggatagtt ctacatatta tgaattggca aaacaggcaa
    11041 caatcggtgg ctgcattagc agtataattt catttggact aatgcttttt atgggaatat
    11101 ttatttttac ccgaaagaaa aaaatcgaaa gctaatgatt gccggacgac ggcaaaagaa
    11161 aaaaaagccg tcgtaatgca gatatatttt catgcgcctt tatagcggtg gctttgattt
    11221 tagagccgcc gtttcttttt gcataaattt agcaacgacg accccatttg accttgcaat
    11281 cagcatggcg gcatatagga ggttgccgtt atgctgagta aaatagcttt tgcttatatg
    11341 gcaaaagaaa gtcttttgtc aaaaaaagtt tgtaccttac aacgggatat tccgggcagt
    11401 ggtatgaact gtcaaatcaa ttaaatactg ctgttatttc ctatgcgtct gtctgcactt
    11461 tgcaggcagg cgcattttgc attttacaga agatttttca aaaactttgc tctctttttg
    11521 gcaaagccaa aagtcgtctg tattatcccg cgaaaagggg aggtactacc gcctttcggc
    11581 agaaaggagg tgattacatg gaacctaatc gcagagaatt tcagaagcaa tgtgtctttc
    11641 aaagtttctg taaacgggtg ttacacaatg aagcctgtaa cgcccatgat gaaatccggc
    11701 gacgcaggaa acgggaagtt tctttttgtg accttgcctt acatgaggaa cggcagcttt
    11761 acactacgga taaatatttt caagctgaaa cagccgagcc gacttatcaa atggcaggaa
    11821 aagaaataac tccgaagtta ttgcttgaag caatacgcac cctgccggaa gaaaagagaa
    11881 ctgctatact tctgtactat ttcgagggta tgactgacgt ggaaatcgga aagatgttca
    11941 atacgtcgcg cagcacgata cagtacagac ggacaagctc ttttgaactg ttaaagaaat
    12001 atttggagga acacgctgat gaatggaacg aatggtaatc aaaacgacta cccggaaaat
    12061 gcccttgttc cctatcccgt tattttggcg gcgagcaaag gcgacccgga cgctatgaaa
    12121 atggtcttgc agcatttcag cggctacata gcaagtcttt ccatgcggaa gctctacgac
    12181 gagcgcggca atgtctatta tggcgttgac gaggatattc gggaacggct gcaagcaaga
    12241 ctgatgaggg ctatcctcac ttttcgggct gaataaccta tgcgatattg gaacgcttct
    12301 ttcccctttt ccgttccata ccgcaagaca gccccctgct ctttgacaaa gaaagcggcg
    12361 caatagaacg gcgacgcagt ataaggcaaa tcggatacgt tccttttgtg ccgagccatg
    12421 cggtgggtac gccatgacct ttttcttaac cgaaaagcga gcgagaaata ccacaccgta
    12481 aagcagggat agcaccctgc gggcgataac gcacaaaata cataatgata cttcctta
//
LOCUS       DAEMHW010000113        12064 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEMHW010000113 DAEMHW010000000
VERSION     DAEMHW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.113"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..525
                     /locus_tag="KPK67_002531"
     CDS             <1..525
                     /locus_tag="KPK67_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2459482.1"
                     /translation="FIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVARLQE
                     HIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIRQLEY
                     KANWYGRKIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASIN
                     ILKEGLRLITIQNK"
     gene            643..999
                     /locus_tag="KPK67_002532"
                     /pseudo
     CDS             643..999
                     /locus_tag="KPK67_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
     gene            1175..1549
                     /locus_tag="KPK67_002533"
     CDS             1175..1549
                     /locus_tag="KPK67_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459483.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKVNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            1641..3101
                     /locus_tag="KPK67_002534"
     CDS             1641..3101
                     /locus_tag="KPK67_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBG2459484.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            3154..6183
                     /locus_tag="KPK67_002535"
     CDS             3154..6183
                     /locus_tag="KPK67_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2459485.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRVAID
                     NPNVSSSNLEYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            6260..8326
                     /locus_tag="KPK67_002536"
     CDS             6260..8326
                     /locus_tag="KPK67_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG2459486.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDENDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKKEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    8919..9129
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9355..10170
                     /locus_tag="KPK67_002537"
     CDS             9355..10170
                     /locus_tag="KPK67_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG2459487.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            10198..10875
                     /locus_tag="KPK67_002538"
     CDS             10198..10875
                     /locus_tag="KPK67_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG2459488.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            10934..11656
                     /locus_tag="KPK67_002539"
     CDS             10934..11656
                     /locus_tag="KPK67_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG2459489.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         4963 a         1143 c         2147 g         3811 t
ORIGIN      
        1 tttatagaaa atcctaagta tcttaagaag tcattaaaca agcttgctaa attacaaaga
       61 gaattatcga gaaaaacaat tggtagttta aatagaaata aagcaagatt aaaagttgca
      121 agacttcaag aacatatagc taatcaaaga aatgattttc tgcaaaaatt atctactaaa
      181 ttaataaaag agaatgatat tatttgcata gaagatttac aagtaaagaa tatgattaga
      241 aatcgtaaac tatctcgctt aatttcagat gtatcatggt cagaatttat tcgtcaatta
      301 gaatacaagg ctaattggta tggtagaaaa attgtaaagg taggtaaatt ctttgcaagt
      361 tcacaaatat gcaataaatg tgggtataaa aacgaggaag ttaaggattt aaatataaga
      421 gaatggattt gtcctagttg taatgaaact cacgatagag atataaatgc aagtataaat
      481 atattaaaag aaggattaag actaataaca attcaaaata aataacgtat gtaagaaccg
      541 taggaactac ggggatggcc tgtggagaat tagtaagaca tattttagta tgcaaaattc
      601 tatgaagcag gaaccctgtg actttagtca tgggaggttc agacagggaa agcagttgta
      661 aaggatactt ctagagataa gattattcaa ataggtaaga tgttaaatga aatgaatagt
      721 tacatgagag tagaaggcca tacagacaat atgtctataa aaaattcaga gtttaagtca
      781 aattgggatt tatcagttat gagagcaacc aatgttgttc aacttcttat tgacaatgca
      841 ggtatagcac cagataaatt atctgctgta ggatatggtg agtttagacc aatagcagaa
      901 aattcatctg aagaaggtag gtcaaaaaat agaagagttg atatagtttt agttgatagt
      961 aaatatgata atgtagaaaa tgtttcaaag gataaatagt taaattagct gtctcaatac
     1021 agaattaatt ctaaaggaga tggctttttt atgcgaaaat caaaaataga tacatagtaa
     1081 gttacctttt tgtaggaata tatggtatct taaatatata atatttaaat aatgtataag
     1141 ctcaattaaa aaatttatag aggatggaaa ataaatggga aataaaaata aaaatactaa
     1201 atataaagat aaaacaagag aaaggtataa taaaaaagtt gagaatttaa ataaagaaaa
     1261 attaaataaa gttaataagc aatctaagaa ggtagataag attgatgata ttattaataa
     1321 atctagtatt gtattatgtg tacttttagt agtgttttca ttgctttact tatttaaact
     1381 ggttcaactt aatataataa tgatactttt agtaattaca gtagctattc aacaaggatt
     1441 ggtgtcattt ggtgcattta gaaataaaaa ttatgtaaaa gctttatttt ctatatttgt
     1501 aatagtatgt ttcttaattg cttttaaata tgttgtaaat cctgtttaaa tatatcaata
     1561 aacgttctat aattaatata aatagtgaag ttacagtttt aatgctatta taaattttat
     1621 aatttgaagg agtagatttt atgaataggt gtaagtttgc aaaattaatt atcacatcaa
     1681 tgattgtttt attacttaca ggatgtagtt taaagaagga agataattat aagttagacc
     1741 cagaaaatcc gacatcaata caaatttggc attattataa tggacagcag aaaatggctt
     1801 ttgataagct tgttaaggaa tttaatgaca ctgtaggtgc tgaaaaagga attattgttg
     1861 aagcattcag tcaaggtaat gtagttgatt tatcagagaa agttttagat tctgcaaata
     1921 aaaaagttgg ttctgaagat gtaccaaata tatttgcagc atactcagat actgcatatg
     1981 aaatggataa attagggtta ctagtagata tggataagta cataagtaaa gaagaattaa
     2041 atgaatacat agacgaatat gtagaagaag gcagaataaa ttctaaggat gagtttaaac
     2101 ttttcccaat tgcaaaatca actgaaattt tgatgttaaa taaaactgat tgggataaat
     2161 ttgcaaaagc tacaaattca aatataaatg atataaaaac aattgaagga ttagtagaga
     2221 catcaaaaaa atattatgaa tggacagata gtttaacgaa agagcctaat gatggtaaag
     2281 cattttttgg aagagatgct ttagcaaatt atattattat aggaagtaaa caactaggag
     2341 tagaaatttt ttctgttaaa gatggtaaag ttacactaaa tattgatgaa aaagttatga
     2401 aaaaactatg ggataatttc tatgtaccat atataaatgg atattttgct tcttatggta
     2461 agtttcgttc tgatgatgca aaaacaggtg atatattagc tcttgtaggt tctagttcag
     2521 gagcagcata ctttccagat agagtaatgc taaatgataa tgagagttat aatattgaaa
     2581 catctgtttt tcaagcacca cgttttaaag gagaaggaaa atatgctgta caacaaggtg
     2641 caggaatggc tgtaacaaaa tctactaaag aagaagaata tgcatcagta gaatttttaa
     2701 aatggtttac agataaagaa cctaatataa aattctctat tgcttctgga tacttaccag
     2761 taaagaaaaa aacgaatgat ttggatgtta taaaagaagt tatgaagaaa gataatttga
     2821 gtttatctga taaagttgaa ggttcaatag cctcatctat tgaacagatt aacagttgta
     2881 atttatatac taataaagca tttgatggtg gaactaaagc tagacaaata cttgaatatt
     2941 caatgcttga taaagctaaa gaagattcaa ataaaataaa tgaattagta aaaagtggaa
     3001 cttctaagaa ggatgcagta gcacaattta ataatgataa aaattttaaa acttggtttg
     3061 aaagttttaa ggctgagctt gaaaatgcta taaaaaaata gatgaattgg cagttagtgt
     3121 gttgacgagc acaaaggtat aggtggaatg ggtatggaaa acaatctaag aaaaaacaat
     3181 tcaataatga aaaagatgat atttccccta attattgtta tgctaattca aacaagttta
     3241 ttttgtgcca caattttatg gggaggaact attaagaaat taaatgataa ttcgtttgac
     3301 atcttaaatg aaagagtaat taatcgtaaa aattatatac aaaatgaaat gttacaaaga
     3361 tggtcaaata caagtgaaac ggaagctaca attaattcct ctgtaaaaaa agtattaaat
     3421 gaaaaaaatg ccttaattga ggatgtaggc aacaatgaag aaattaacaa agatattata
     3481 aaaagcatat caaatgattt aatatatctt ttgagaaaaa attctgttac aggagctttt
     3541 ataatcctaa gtgacaaaaa tattaacgat aagtcaatag atagtaaagg tgaaataagt
     3601 aggacaggat tgtatataag ggatttagac cctaagttta atccaaatga taattctgat
     3661 ttgttaattg agagaggttc atcagatata gcaagaagtt ttggaatatc tatggacagt
     3721 tattgggaac ctaagttttc atttaaaagt gaaaatgagc aagatggaga tgattttttt
     3781 tataaaccat ttagagttgc aattgataat ccaaatgtat caagttctaa tttagaatac
     3841 tggggaagta agtttttatt gacaaatgca ggtgataggc ttatgacata tacagttccc
     3901 cttatttatg aagatggaac tgtatatgga gtacttggaa tagatttaag tgtaaattat
     3961 ttaagagaat tacttccata ttcagagata aatgggaata aaaagggtaa ttatatttta
     4021 ggtgtagata gaaataatga tatggccttt gaaaatgtaa tttcaaatgg atactcatta
     4081 aaagaaaaat tttgggatgg tttacattct aatttagagc agaaggaatc gtataaaaat
     4141 atatataaga ttaaaaataa agataatgaa tgtggtaatg gcaacattta tgctagtgta
     4201 caatacttag atttatataa ctccaatact ccatttgaaa atgaacgttg ggctcttttg
     4261 ggtattattg aagaaaaaag tcttttgtca ttttcaaaaa gggtagagaa attagttata
     4321 atatctatag caatttcttt attaataggt ctaataggta tttatattgc atcaagatta
     4381 tttacaaaac caattgttct tttagccaac aaagttagaa acagtaatcc atcaaaacca
     4441 gtaaatcttg ataagataaa tatatctgaa atagatgaat tatcttcagc tatagagttg
     4501 ctcagtgcaa atgttgcaga ttcttcgtca aaattatcac agattattga aatggtagat
     4561 atgccaattg gggcttttga gttatacaaa aacgcagaga atgttttttg tacaaaagaa
     4621 ttttttagcg tgtttactac tgaaatcaaa tttgaagaat atggatatat ggaaaaaaat
     4681 aagttcctga aaaaattaag gggactagat aaatatttgc aagaaagtta tgaaacagaa
     4741 aatacatata tatataagtt tgaatctaaa gataaaaaac ttaagtggat aagaatgaat
     4801 atctttgaag atgagctaaa ggttcttggt gttgttgttg atgtaacaaa agaatttatt
     4861 gagaaaagga agatagaata tgaaagagat tttgatattt taacgaatct tttaaatagg
     4921 agagcttttc attcacagat acaagaaaaa tttaagaata aagatgaact taagatagca
     4981 gcatttatta tgtgggattt agataatcta aaatatatta atgatacata tggacatgat
     5041 gctggtgatg aatatattag gtgtgctgca gacatactta agaagtttat atcatggaat
     5101 ggtatagtat ctagactttc aggtgatgag ttttatgtat ttatatatgg atatgaaagt
     5161 aaagatagta taagagatat tattaattct gttaagaaac agatgaataa taccttatta
     5221 aaacttcatg atggaacaga atttaaaata agggcatcag caggaatttc ctggtaccct
     5281 gatgattcaa ccaactatga tgaattaatg agatacgcag attttgctat gtatcaaatt
     5341 aaaaatacaa tcaagggtga agttagtgaa tttgatatta atatatataa taaggattca
     5401 ttcttattga gtaatagaga agaattaaac aagttaattg atggtcaatt agttgagtat
     5461 gcatttcagc ctattgtaga ttgtaaaggt aaagtttttg catatgaggc acttatgaga
     5521 cctaaaataa aaacttttag ttcaccaata gatgtcatta gattagctcg ttcacagtct
     5581 aaattatatc aaattgagcg tattacatgg tttaaagcac ttaaatcatt taaaaaatac
     5641 aaagaaaaat ttggcgattg caaactgttc ttaaattcta ttcctaataa taatttgtca
     5701 aaagtagatt ttgaggaaat tgaagattta tatggtgaat atttaaatag agtagtacta
     5761 gaaataacag agaatgataa gattaatgaa gattttattg agaaaaagaa agcatatata
     5821 gaccattgga atatagaaat tgctttagat gactttggga caggttacaa cggagatgct
     5881 gtactattat atataatgcc agactatgta aagattgata tgtctattgt aagaggtatt
     5941 gataaagatg aaaacagaca gaaaatatta aaaaatctta tctcatattc taacgaaaga
     6001 aatattaaag tgatagcaga aggtgtagaa acaaaaggtg aaatggaaac actaattaat
     6061 cttggagtag attatatgca aggctactat attagtaaac catcatttat acctgaagat
     6121 ataagcgaca aaattaaaaa agaactcaag atatgtcatg aaaataggaa tgatgtggtt
     6181 taatagagga aaaattaaat ttatttttat gaacttttag tttataatta aaaagaaaat
     6241 aatataatta taaatcaagg tggtggagct aataatcaca acaaatatag atataagaaa
     6301 gttaaatgaa aatcaattag aagcagttga gcatatagat gggccttgca tgatacttgc
     6361 agggcctggt tcaggtaaga ccagagtaat tacatataga atagccaata tggttgtaaa
     6421 caaaaacata gcaccaacaa gaatcttggc aataagtttt accaaggcat cttcaataga
     6481 aatgaaaaat agagctttga atttaagtga tgacataaga cttaataagg taacctatgg
     6541 tacttttcac tctgtatttt ttaaaattct aagatacttt gaaagataca acttagacag
     6601 tatatttgat gaaaagtcaa agcgaatgac aatcaaggca atcctaaaaa gtttaaatat
     6661 agagaatgct gatgatgatg agaatatagg acaagttata aatgaaattt cctatgtaaa
     6721 aaatgaactt atggataaaa atgaatttaa ttcagaagta ttaacaaaag atgagttttt
     6781 aaaagtatac aacttatacg aagaacaaaa atctaaagtt aataaaatag attttgatga
     6841 tatgcttata agaacttatt atttactttt gaacaataag tctgcactag aaatggttag
     6901 aaatgtgtat aaatatatat tgatagatga atttcaagat ataaataaag tacagtttga
     6961 agttttaaag ttaatttgta gcccattaaa caatatattt gcagttggag atgaggacca
     7021 aagtatatat ggatttagag gtgctagacc agattttttg cttgaatttg aacaatactt
     7081 taataatact aaaaaaataa tattagatat aaattacagg tctaaaagtg agatagttca
     7141 tactgcaaat agacttattg acaaaaataa aaatagatat gaaaaaatca ttaagtgtag
     7201 tcagggggat ggaggaagtg ttacctatat ttctccgcat gattcagagg aagaagcctt
     7261 atatattgcc agggaaataa ttgacgaaat acaaaaagat tatgtagagt attcagattt
     7321 tgcagtaatt tatagaacta atatacagtc aagagcatta gtagatgtat ttatggatat
     7381 gaggattcca tttgtagtaa aagactcagt aattacaata tatgaccatt gggcaagtca
     7441 agatatacta gcctatttaa gaataggtat taatccaaaa tcaaataaag attggttgag
     7501 aattataaac aagcctttta gatatatatc caaagacagt ataaatatgg ttaaggatga
     7561 aaatgacttt ataacagcat taataaataa gtgtaatctt catcctaaac aagttaaaac
     7621 aataaatgat ttggaaatag atttaagtta tttaaatact ttaaatccta aaaatgcaat
     7681 atcttatatt agaactagtc ttgattatga tagatatgtt ttagattatt gtagcaacag
     7741 aaagataaag actaatggac ttgtagagat attaaatgaa cttgagagtt cagcaactaa
     7801 ttttaatact gttacagagt ttttagagca tattgataga gtaaagactg agttaacgga
     7861 aaataacaag aataagcaga ctgagggtgt catatttaca actatgcata gtgcaaaagg
     7921 attagaattt agaaatgttt atattatagg tgtaaatgaa ggaacaatac ctcatgaaaa
     7981 atcatatgat atttgtaagg aagagaaaaa aaaagaacaa ttagaagaag aaagaagact
     8041 tatgtatgtt gctattacaa gagctgaaga aaagttatgt ataagctcta ctttaaataa
     8101 gtatggtaag aaagttgata aatcattatt tattaatgat ataaaatctc caacgaaaaa
     8161 agaaattgac agtattggtg taggggataa ggtataccat aaaaaatttc atgaaggaga
     8221 aataattaaa aaagatggaa ttatgtttac aataaagttt aaagatagag agcgaatctt
     8281 ggatttgaaa acttgtttat tgaagaatat aatttatact atttaattta gaatctaaat
     8341 ttagatagga atataatgta ttatttatta tgtgcaagac aaatagatat gtgtaaaata
     8401 tattatttaa cttattagaa aaagaaataa gggtttaata tattatttat agttgtttag
     8461 aaaaagcaac aatgtggaaa atattattta gtattattaa agaaaagcaa caatgtgaaa
     8521 gatattattt agtattatta aagaaagagc aacagtgtga aagatactat ttagtattat
     8581 taaagaaaaa gcaacagtgt gaaagatatt atttagtgta atttaagaaa agcaacaatg
     8641 tgaaagatat tatttagtat tgtttaagaa aaagcaacaa tgtgaaagat attatttagt
     8701 attatttaag aaatatctaa aaatgtgaaa gatattattt agtattattt aagaaatatc
     8761 taaaaatatg aaatatacta tttataatta cacaaataat atcaataaat ataaacagta
     8821 gtatttagat aatttacaag taaaaattat taaaaaaata aaaaacttat tgacaaaaat
     8881 attataagga agtacaattg tattaacaat ttaaggaaac aaatccaatg atagagaaag
     8941 tagcatatat ggatttacag cgagttaggg gttggtgtaa gcctaacaat gaagtctatg
     9001 tgaagagagc tctggaggag attatcagaa actttagtat gataattggt ttacccgcct
     9061 taagggattg agaggataag tacttactta tcaaaaagag tggtaacgcg gatataattc
     9121 gtctcttagc tgtaaagcta agggactttt ttgattttaa aataaaaata tccatacagc
     9181 ttaaaagcta acagtcacaa attttgaaag taatataagt atttatcaat aagagtggta
     9241 acgcggtaaa gtcgtctctt agtcattaaa gactgagaga ctttttttgt aactcgaaac
     9301 atttaagtat taaaatctga gggaaattat aattaaaaga gaggggtagt taaaatgaaa
     9361 gtatttaaaa aattattaag tttaggatta gtattaggat tgacattatc gctagtagga
     9421 tgtagtggtg gaggagaaaa aacaaagtta gaacaaataa aagacaatgg aaaattagtt
     9481 gttggtacta gtgcagaatt tcctccattt gaatttcata aggtagtaga tggaaaagat
     9541 tcaataaaag gattcgatat aatgttggct gaagaatttg caaaagagct tggtgtaaag
     9601 gttgaaatta aggatatgtc atttgatgga ttaataggtg ctttaaatgc agaccaagtt
     9661 gatattgttc ttgcaggtat gtctccaaca ccagagagag aaaagagtgt tgatttttca
     9721 gaattatatt atttaagtag aaatgcagtt atagttaaag atgcagatat agacaaagta
     9781 aaaactgaag atgatttaaa gaaacttaga gttggagttc aagcaggaag tatccaagaa
     9841 gaatatgtag ttaacacttt aaaaatgaca actacaaaat ctttgaaagc aatacctgat
     9901 ttaattacag aattaaaaaa tggaaacata gatgcagttg ttactaatga agcagtttct
     9961 ttaataaatg ttaaaaaata tgatggaata aaaatggcaa atactgaagt tgggaaagat
    10021 gtaacagaag gtatggcagc agcaatcaaa aaatctgaca ataacaaaga ttttatagaa
    10081 ttattaaaca aaaagataaa agaattacaa gatggcaaga agatagaaga atttttaaat
    10141 gaagcatcta ctgaagctgc ttcaaattag taagaaagtt atgtgggaga tgaaattatg
    10201 agtttagatt ttagtttttt aagtagattt gggacttctt ttttggaagg aacaggtgtg
    10261 acagtatcaa tttctcttgt ggcattatgc tttggattta taataggtat aattatctgt
    10321 atggcaaaaa tatcaaagag caaagtatta agagcaatat catcaatcta tattgaggtt
    10381 ttaagaggga caccattatt agtacagata tatatagtat ggtttggttt acctcaatta
    10441 ggaataagat tccctatgtt gtttggtata ccaagtgaat ttatagctag tgcatttgca
    10501 ttatcagtaa actctggtgc atacgtggca gagatactta gatcaggtat acaatcagtt
    10561 gataatggac aaatggaagc aagtagatca ttaggattaa attattggtc aactatgaga
    10621 tatataataa tacctcaagc tataaaaaat atattacctt cattagcaaa tgagtttatt
    10681 acacttgtaa aagaatcttc aataatatct gtaataggag ttgttgagat aatgcgtact
    10741 gctgatatag taaaaaatgc agcatttaga gcattagagc cattaatagt agcagcagca
    10801 atttactttg ttataacatt tactctatca agattagttg gattattaga aaagaaacta
    10861 tctgtgtcta actagatgag agcatgttta gcatttaggg aaaacataga atagtaaaaa
    10921 ggaaagtgat aatatgatta caataaaaaa tttaagtaaa tcttttggag atttaaatgt
    10981 attaaaaaat atagacttag aaatcgctaa aggcgaaata atggttatag taggtccaag
    11041 tggttctggt aaaagtacac ttcttagatg tatgaattta ctagaaatac ctacaggtgg
    11101 agaaataata tttgaaggaa aaaatttggt cgataaaaaa actaatattg atgaggttag
    11161 acaaaatata ggtatggtgt ttcagaactt taatttattc ccacataaga ctattttaga
    11221 taatataaca ttagcaccaa ttaaattaaa aaagatgact aaggaagaag cagaaaaaaa
    11281 agcagaaatt ttgttgagta gagttggtct tttagataaa aaagattcat atccatcaca
    11341 attatctgga ggacaaaagc aaagaatagc aatagctaga gcacttgcta tggaaccaga
    11401 tatgatgttg tttgatgagc caacatctgc tcttgaccca gaaatggtaa acgaagtttt
    11461 ggatgttata aaagaacttg ctaaagaagg tatgacaatg gcaattgtaa ctcatgagat
    11521 gggatttgct aaggaagttg cagatagagt tatttttata gatggaggaa gtatacttga
    11581 agataatact ccagaagaag ttttcggaaa tccaaaacat gaaagaacaa aagcattttt
    11641 agcaaaagtg ctataattat ataaaaagga tgttattgat attagaaatt caatacatcc
    11701 ttttttagtg atggttaata ttaattttaa aattaaagtt atattaatat taactttatt
    11761 tttacgattt tttgcagaaa aaaaaagagc tcggggtaag ctcttttttt ccaccgaatt
    11821 aatttccgtt actaatccga tttttaataa atataaaata tatccttatg aaagggtatt
    11881 gggaatagat atattaacta actagggtta ccagggggat aaccttaatt tattataaca
    11941 aaatgcgaca caaaaatcaa tatttttttt gaaataaatt ataaaagtta tttttataac
    12001 aagtaagctt ataaatttaa cttttatagt aaaaataagt atatgtataa tataatatat
    12061 ttat
//
LOCUS       DAEMHW010000114        12013 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEMHW010000114 DAEMHW010000000
VERSION     DAEMHW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12013
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.114"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            62..1261
                     /locus_tag="KPK67_002540"
     CDS             62..1261
                     /locus_tag="KPK67_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBG2459490.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKNIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            1313..1816
                     /locus_tag="KPK67_002541"
     CDS             1313..1816
                     /locus_tag="KPK67_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2459491.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
     gene            1923..2240
                     /locus_tag="KPK67_002542"
     CDS             1923..2240
                     /locus_tag="KPK67_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459492.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            2398..3357
                     /locus_tag="KPK67_002543"
     CDS             2398..3357
                     /locus_tag="KPK67_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG2459493.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            3472..4257
                     /locus_tag="KPK67_002544"
     CDS             3472..4257
                     /locus_tag="KPK67_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459494.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            4549..5868
                     /locus_tag="KPK67_002545"
     CDS             4549..5868
                     /locus_tag="KPK67_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2459495.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(5943..6371)
                     /locus_tag="KPK67_002546"
     CDS             complement(5943..6371)
                     /locus_tag="KPK67_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459496.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(6462..7838)
                     /gene="hydG"
                     /locus_tag="KPK67_002547"
     CDS             complement(6462..7838)
                     /gene="hydG"
                     /locus_tag="KPK67_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBG2459497.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(7839..8924)
                     /gene="hydE"
                     /locus_tag="KPK67_002548"
     CDS             complement(7839..8924)
                     /gene="hydE"
                     /locus_tag="KPK67_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBG2459498.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLDVDYSRGDNIEWRRL"
     gene            complement(8960..10168)
                     /gene="hydF"
                     /locus_tag="KPK67_002549"
     CDS             complement(8960..10168)
                     /gene="hydF"
                     /locus_tag="KPK67_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBG2459499.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(10183..10434)
                     /locus_tag="KPK67_002550"
     CDS             complement(10183..10434)
                     /locus_tag="KPK67_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBG2459500.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(10815..11855)
                     /locus_tag="KPK67_002551"
     CDS             complement(10815..11855)
                     /locus_tag="KPK67_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBG2459501.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         4407 a         1497 c         1820 g         4289 t
ORIGIN      
        1 ttgaaatttg tacctaaaaa gtaaagtaat ctagaattaa gaaaatggag atagaattat
       61 aatggaaaaa ttatattata caaatcagta tataagagat tttacagcag aaataattga
      121 aataaaagaa gtagaaaata agtatcatgt tttattagat aagacagctt tttttcctgg
      181 tggaggaggt cagtttggag acctgggaaa aatagagaat gatgatgtaa taaatgtgta
      241 tgaagatgga aaaaatatat accatgtatt agataaaaaa cctataaaga tacataaggt
      301 aaagtgtagt attgattggg atagaagaga agatggaatg catcaacatt ttggacaaca
      361 tgtattgtct ggatgttttt ttaaaacttt taatgcaaat acgacaggtt ttcatttggg
      421 aaatgagtat agtacagtag atatagaagg taatttagaa gaggataaaa ttagagagat
      481 agagttgttt gccaatgaaa ttattcgtca aaatatacaa gtagagacat tacttccaac
      541 gaaaaaagaa ctaaaaaaga tttggttaag acgtgatttg ccaaatactg atgaagagat
      601 aagagtaata aaaataggtg atttagatac gaatgcatgt tgtggagtgc atccaaaatc
      661 aactggtgaa ttgagaatga taaaaataaa gaaatgggaa aaacataagt caagtacaag
      721 aatagagttt ctagcaggta aaagggcagt ggatgaagta ttgaagaggg atatatattt
      781 gactaaaata tgtagatatt taagctgtag tgaggaagat gctataaatg gtattgtaaa
      841 tctaaatagt aaagtggaag aggctttaat taaaaaaaga aggcttgaag aagtagtatc
      901 tacatatcag gtcaaagaaa tgattgaaaa agcaaataag gttggcaata tttcggtcat
      961 taaaaaaatt tatgatgatg aagatttgaa atatgtaaat aagatagcaa ataaaattgc
     1021 agaatatgaa aatagtattg cattattagc tgttaaattt aatgataaaa taaatttagt
     1081 ttttgcatgt tctaaaaatt taaaagatat taatatggga gttttattaa aggatgcaat
     1141 aagtcttata gatggcaagg gtggtggaag taaagtatta gctcaaggag gaggtaagaa
     1201 taatggaaat ttagaggcta cacttgatta tgcatttatg aagatagaaa aaactctata
     1261 aatatattat ataaaaaacc ttagtagata aaatagatat ggaggaataa gtatggagca
     1321 acaaaaggac tacatagagt tatatgaaaa tgaagaatta atagagataa gagaagcacg
     1381 tatggatgat ttggatacaa tagcaaaatt taattataat ttggctaaag aaactgaagg
     1441 aaaagaactt gatatggatg ttttaactaa aggtgtaaaa gcattgttat tagatgagag
     1501 aaaaggaaaa tatcatgtat atacagtatt tgataaagtt gttgcacaga ttatgtatac
     1561 atatgagtgg agtgattgga gaaatggaaa tttcctttgg attcagagtg tatatgtaga
     1621 taaggaatat agaagaaaag gtatctttaa ttatttattt aattatataa aaaatatatg
     1681 tgataaagat gaaaatatag ttggaatgag attatatgta gaaaaagaaa acataaatgc
     1741 aaaagcaaca tatgagtctt taaatatgta tgagtgtgat tataatatgt atgaatacga
     1801 agtagtacat tcgtaatata aatttgtaat actgttatac tgatgttata aagtattgat
     1861 tatattgacg attatatgaa ataaactgga tattataatt agaattttta gggggtataa
     1921 aaatgggcgt tttaaaagga atgttttatg tatttttatt tttagattta gtgtcaatat
     1981 tctgtttctt tttcaataaa ggaaaaatag catctaataa aattgtgttt aatgcaatag
     2041 gagtacttac atttgtactt tgttttatgc tttttagtta ttatcctaat aataatctta
     2101 ttggaaaatt tattgcttct ctatttttta tatttggagt tgcaggagtt atgttgaaag
     2161 aaaaaaattt tttatatgct agacttcttt taactgtagt tatagttttt tctacattaa
     2221 gattgttcgt tattcaataa aatgtatttg tatgaaaatt tattaaagtc tacacattta
     2281 agtgtaggct tttttgattt tttgtttgaa aattaaggaa attttaagga aattttaaaa
     2341 tcgtcttaaa tatttattga tatacttaaa atattaatag cgaaaggaga cttatcagtg
     2401 atagaaagta aaaagtcaga aaaattgtta ctgtcaatta tatacataat atttttggta
     2461 tatattttaa tgattataag aataatttta tttagagatg taccaattta cgctattttc
     2521 aagggaacgt ttaggaatgt aaatttaatt ccattttata caatatatca atttatagta
     2581 gatagtaatc ttgactttat gaaagctact gtaaatatta tagaaaatat aggtatattt
     2641 attcctatgg gaattttttt acctatagta tgtaaaaatc taaataaaaa aactataatt
     2701 atagtaatta tacttgttag cctagcattt gaattaacac aatatatttt tgctcttgga
     2761 agtagtgata tagatgatgt tattttaaat tcattaggtg gcataattgg aattaatatt
     2821 tacataaaca tgaataaatt atttcccaat gatattaaca gatttaaggc tataataggt
     2881 actagtttag ttttaggagt tataggatta ggagttattt ctaaaaatta tcataactta
     2941 ctaactttta aatttagacc tgatagaaaa atatcaaaaa tattaattga agaaaatagg
     3001 gaaataatta aagatataaa taaagataca gtagatatag ttgggacatt tgagagcttt
     3061 aaaaaaggaa taataactat taaagcaggt tctaataaca aagtaaagag accagagaat
     3121 ttaattgatt ctgatggaaa tataagaatt tatttaaatg aaaatacaaa attagtatct
     3181 tatataatta ccaaagaatc aaaaatggat atagttaaat atgaggaatt tgatgtgaag
     3241 aatttagatt tattaaggag atatgatact attaatgttt ggattgataa agaaaatcag
     3301 ccaaaggata aacatggtat tatggctagt aaattattaa ttggattgta tgaataaaat
     3361 attaaacaag aataaaagaa taacaaatat aaaaaacgaa aaaactattt ctataattat
     3421 ttttacgttg tgcataaaat aagaacatta gtgaaggagt agagataaat tatggagata
     3481 ataaaatata tcttgtactt ttttatatat tcatttttag gatggacagt agaaagtata
     3541 ggatgttcta tagcaagcaa aagaataatc aacagaggtt ttttaaatgg tccaatatgc
     3601 cctgtttatg gttttggagc ggtaattgtg atatcactat tgggaagatt taataatgta
     3661 gtaatagtat ttctattagg aatgatttta actacgatac tagaatattt tacaggattt
     3721 attttagaaa ccttatttca tgctaaatgg tgggattatt ctgacagaaa attcaatata
     3781 aagggtagag tatgtttaaa aaatgcaata tactttggtg taatgtctgt tttaataata
     3841 agatttatac atccatttat aaaatatttt gtaagtataa ttccatatag aatattaatt
     3901 tcaatggcta taattacaac actttggact atacttgatt tgattgttac tattataaca
     3961 cttaaaaaat tagatataaa gttaaactta ttagacgata taattacaga ccttaatgat
     4021 ataaatgtga aattggataa atttgataga ggagaaatac aagctttatt taaaacaata
     4081 aataacgacg aattagaagt tagagaaaaa ataaacaaaa taaatagtaa gttggaccgt
     4141 atagagtcta atattatttt acaaaaaaga gttataaaag cttttccaca tataaaacat
     4201 aaaaaacaac aagagcaact agaacatttt aaaaaaatta ttaatgagaa aaggtaatta
     4261 tacaatttaa tgaagacgaa tagaataatg gtatttataa gatgattttc agaggactct
     4321 ataaaaatag aatagagtcc tttatttttt gaaaaataat gtttatttga taaaaataaa
     4381 atatattgtt tctgaataac aaatattttg aaaattataa aaacatatat tgacaatttg
     4441 tgtcaaaaat actaacatta agtatataaa gaattttata tgagaatatc tttataaatc
     4501 gcaataattt taaaacataa atttatttaa agaggggatg atttgccaat gagcgagata
     4561 actagtcaaa tgataagtac agaagaaaaa aaatatgttg ctaaaactca aaagatacct
     4621 tattatccag tagcatttaa atcaggtgat ggtgcaatgc tatatgatta tgaaggtaat
     4681 gaatatgtag attttttagc aagtgcaggc tctgcaaatg taggtcatgg taataaggaa
     4741 atctcacaag cagttaaaga acaaatggat gatataacac aatatacact tgcatatttc
     4801 catagtgatc ctcctgtaaa attagctgag aagcttgtgg aaatagcccc tggagataat
     4861 gataaaaaag tactatacag tgcaactggt tctgcttgta tagatgctgc tataaaatta
     4921 gcaagagggt atactggaag aacaaagata atatctatgt gtgaatctta tcatggaagt
     4981 acttatggag ctatatctat atctgcttta agtacaaata tgagaagaaa aatgggtcca
     5041 ttgttaccag aagtttatca cttccattat cctgataaaa atagaacggc taaagaatgt
     5101 ttagatgaga tagaatatgc ttttgcacac tatctaccag cagaagaggt agctgcaata
     5161 tttatagagc caattgcagg agatgcagga ataatagtgc ctccagtaga gtgggtacaa
     5221 ggtttaagta aaatatgtaa agaaaatgga atattacttg taagtgatga aatacaacaa
     5281 ggtatgggaa gaacaggtaa gtggtttggt atagaaaatt ttggtgttga agcagacctt
     5341 atagttcttg gaaaatctgt tggaggtgga ttacctctag gagcagttgt tggaagaaca
     5401 gaaataatgc aaagtcttga tgcaccagca catttattta ctttagctgg aaacacaaca
     5461 gtatgtgtgg ctgctttaaa atctatagag ataattgaaa aagaaaactt acttcaaaag
     5521 agtatagaaa tgggagatta tattaaagca ggatttgaaa aactaaaaga aaaatacgat
     5581 ataattggag aaataagagg cataggatta tctataggag ttgatatagt taaaggaaaa
     5641 ggttctaatg aaaaacatcc agatgctaca gctaagatat gttatagatg tatacaaact
     5701 ggattaataa tgatattctt aggacaatct actttaagag tgcaaccacc tctagtaata
     5761 actaaggagc aagtagataa agcaatgaac atcatagatt ctgcaataga tgattattta
     5821 aatggtagaa ttggtgatga agtatatgaa gtaactcaag gttggtaaaa taaatattaa
     5881 atgaaaatag gagttatctc aattttagaa aattatttct gaaatgagat aactctttta
     5941 agttacccac aaataagaaa attgtttgat tcattaaaga ctatatttcg gatgtcgttt
     6001 ttgtgagtat cagagacagt attacgagaa taattacctt tattcgtact atctgttaat
     6061 ttagttataa ttaagatagc taaaagaata agaattactg tgtataaagc aataacatat
     6121 ggttttcttt tatatttgta tttgttattg aaaaaagaat ggccataatt atttttattt
     6181 actttagatg tatttgtatt ctttttcttg ttacctttct ttttatcatc cttttttcta
     6241 tttttagggt aaggaatgac tttgtgtgta ttatcaagtt tgatattttt atttttttga
     6301 atattgtttt ccaggctagc ctttttttct ccaaattgaa tagttttatt ggtattgttg
     6361 ttatctttca atgttaatct ccctccctta aactttccct ttagataata ttactactaa
     6421 cctatggaat atacaataaa taatatataa gtaataaaat tttataggta taaatctctt
     6481 tctccacgct ctaaggcagt catattgtta gatactatgt ttcttatatc ttctctttct
     6541 atcttaccta tttcttctgc gataagtttt ttacccataa ttttaagttc ttcatctcca
     6601 taatcgatta aaaattcatt taatgtagta agagcatttg gtgtacatac attatggatt
     6661 tgtcgacttt tagctaagct catgaatcta tcgccagttc tacctttacg ataacaagct
     6721 gtacaataac taggtatata tccatcagta atcagttctt taagcacctc aacagggctt
     6781 ctatggtctc caacttcgaa ttgttctgta ttgtcaccat cttcatatgc tttatatcct
     6841 ccaacacctg ttagagaacc tgcactaatt tgtgatacac catattttag taactcattt
     6901 ctcatttcag cagtttctct agtggacatt atgattccag taaaaggaac agctaatcta
     6961 gttatagcta ctattttttt gaacatatcg tcagaaacta agtgaggaaa atcttctaag
     7021 ctcatacctt cagctttttt caatctaggg aaagatatag tatggaaacc tacaccaaat
     7081 ttttcctcta agtgttcgtt gtgcatcata agtcctagaa cttcaaattt agggtcagat
     7141 aaaccaaata aaactcctgc accaacatca tcaacaccag cttccatagc tctatcaaaa
     7201 gctgttaaat ggtaataata gtcatttttt atagattgac catgcatttt tataaatgtt
     7261 ggtttgtgat aagtttcttg gaataatata taagttccta ttcctttttc ttttagtaat
     7321 ttatactcat ctacacttgt agctgctatg ttaacgttta cccttcttat atttccgttt
     7381 tcattatatg ttgaataaat tgcatctaag caatctaaga tgtaatttat atcacaattt
     7441 ttagggtctt caccagcttc tagagcaagt cttttatgac ccattttttc taatatttta
     7501 acctcttcct ttatctcttc catggtaagt ttttttcttt taaatttgtt gcatttacta
     7561 aaaccgcaat aaacacattc atttacacag tagtttgata cataaagagg ggcaaataaa
     7621 accactctat taccatatat ttcatttttt atttgcccag ctatactaaa taatctatct
     7681 agttgttttt tatcttctac ttcaagaagt gtagctatat ctttataact aagtttttcc
     7741 cttctatcag ccttatcaag taccttttca atatcatcag aagttgaatt ttttgcatct
     7801 tctaataaag aattaataag ttcatggttt atgaacattt acaaacgcct ccattcaata
     7861 ttatcaccgc gagaatagtc aacatcaagt cctaatgact tgatgttttc ttctaaactt
     7921 tggtgatatt cacaagcttc ttgaccagta aaggctttgt tatcgtaaag agaatattgt
     7981 tttctaaatt cttgaggaga aaggtttggc ataattacat tgcaaccagc taaaagacct
     8041 gcatttctac cactagggtt tatagatgcc aaggctgttg tagcgggtaa taaaactttt
     8101 ggaagtagaa gtcttgtaat tgaaagcata agtaatgtct tctctaaatc tccatgttta
     8161 taatccttaa aaattgtatc atggtgtgga ataaaaggtc ctattccaac catatgagga
     8221 tttaactctt ttaggtataa taaatcacga acaaggtctt cgttgttctg aaatggtgat
     8281 tcaaccataa atccagcacc tacctgataa ccaatttctt taaggttttt taggcattct
     8341 tttctagttc taagctcaat gtttggcgga tgtaattttt tataatgact gtctgtagca
     8401 gtttcatgtc ttaaaagata tctatctgca ccacataaaa agtatttttt atacgaatca
     8461 taactctttt ctcctagtga tagagttatg gcacaatcct catatttatt ttttatactt
     8521 gaaacaattt cacatatttt atcatcagta aaatatgcat cttcaccacc ttgcaaaacg
     8581 aatgttctgt accctatttt ataacctata tcacaacatt caagtatttc atcaagtgaa
     8641 agcctgtatc ttatgatatt tttattagaa gattgtattc cacaatagta gcaattattt
     8701 ttacaatagt ttgtaagctc tataagtcct ctcaagtaga ctctatttcc ataatgttta
     8761 tttctcatgt cgctagcttg tttataaaga tagtttctta aattttgttc gaagttatat
     8821 tcctcattgt cgatattttc tattaaatat agtagttcat caaaacttaa tgaattaatg
     8881 ctgtaaagtt tatctataaa gtatttaatc ttatttttag tcatattcaa atatgtaatt
     8941 attaagtata gttaaatttt taataattga aaagttcaag tgatctttcc aaaatacctt
     9001 ttacatatgc tattaagaca ccatagttta caataggtac attgaaagat tttgcctttt
     9061 ctattctaga taacattcca gctctattca tcatacaagc tccacagtga actacaaggg
     9121 catatttatt tatatcttca gtgaaggata ctccagaact aaattcaaaa gtaatgtttt
     9181 tgccagtttt ctttctaatc atattaggaa tttttactgt tccaatatca tcagtttgtc
     9241 tatgatgggt acatccttcg gccatcaaga ttttatcacc atcttttaaa ttttctaaag
     9301 cataagctcc attaatcaat tctttaaggt ctcccttttg tctagcaaac aatatagaaa
     9361 aagatgtaag aggaatatct tttggtgtat ctttatctac ttgtggaaat acttgagagt
     9421 cagtaataac aagttttggt tttttgccta aattagatag agtttctttt aaactatctt
     9481 cttttgtaac tatagaaatt gcaccattat ccaaaatatc tcttataact tgctgttgag
     9541 gaagtataag tctacctttt ggagctgctt tatctattgg aacaactaaa acaactaaat
     9601 catttggttc aattaaatct gataccaatt tgaactcagt gctatcctta ggcaaaactt
     9661 ttattatttc attttttaaa ttttcaattc caattttatc tgttgatgag acagaaacaa
     9721 ctggacattt aactttattt ttagttaagt ttaaaatttc tgattgattc ttaatagtat
     9781 caattttgtt gagaattaga atatgaggga tattcttatc attaaacttt tcaattagtg
     9841 acaaatcttc ttgtgatata ccaatctggc aatccactac tagtaaagct atgtctgtct
     9901 tttctaaaac atctagtgat ttacctattc taagttcacc aagttcacca acatcatcaa
     9961 gaccagcagt atctataaga acacaaggtc ctataggaag gatttccatt ggtctaaata
    10021 ctgggtctgt agtagttcca gcaatatctg agactattgc agcactttga tttgttatag
    10081 catttattat gctagattta cctgcatttc ttttaccaaa gagacctata tgtactctta
    10141 ccgattgtgg agtagaattt agactcattt gtactcacca ccttaaactt ctttttttga
    10201 tatactagtt tttacagata catgaggtat gttgcccaat ttaccagtca aactattaat
    10261 agtatccatt tcgccaacaa ctgctataca tacaattgag acaccttctt cttcgcaagg
    10321 aatgcccatt cttcctttaa taataccttt aaaacctgat acaacctcat tgaatttaaa
    10381 ttgacattct tttggctctt ccaaaatagc acttataact gcaacctttt tcataaatat
    10441 cctcctaact cagaaaatat gtcttaaaca aatccaaaat caaaaaactt ttctttgtaa
    10501 agaaaagttt ctacaaaaga aaagttatta attacacaag tataatgtgc tatattaaac
    10561 tatttcctta cagattagat aactcttttc tggtagggtt agcttgtaaa aatttaattt
    10621 aacaacaagt aactgaagaa aatttttata caatttatat aaattataat aaaaattaac
    10681 taaagaaaat tgaagttact tatataatag tagtaaattg aaattaagtc tatagaaaaa
    10741 ccaatttttg tataaaaata aaaattaaag ttaattattt gttaagaatt gactttggta
    10801 aaatatataa ttaattattc tttaagcata taatttataa gtaattttag tgtattttca
    10861 atagcttcta tatgtgttct ttcataatgg tgactattgt ttgttccagg accaacacat
    10921 gctatttgaa catcttctcc ccaacgaata gcttctgaag catctgaacc atagaaagta
    10981 aatacatcga cagcgtagtc taaattattg tcttctgcaa catcgacaag cttgtttctt
    11041 aaattaaaat catataatga taaattatct tttgcaacta tagttacacc atgttcacta
    11101 gatgtttgac ttggtccagt aggtgctata tctattgcta caaattcttt tgttttttca
    11161 ggtaaacatt tagaaacacc atgtccaatt tcttcataat tacttatata aaagttagta
    11221 gtatgcttag gtgttaaatt gttttcttta aaatatctac atatttcaag aaccattgct
    11281 acagctgatt tattatctat atatctagat tttataaaac cagattctgt agcctcaaat
    11341 ctatggtcta ggcatacaaa atccccaaca cttattccta aatctaatac atcttgcttt
    11401 gtaaatactt tttcatcaag tctaataatc attgtttctt catttctttc agtggcagct
    11461 tttgtttgac catatatatg agtagaagca tacttaaata caacacttcc tctgattttt
    11521 ttaccttttc tagttataac agtacagttt tctccctcta tagctgccca acatccacca
    11581 ccaactttat ggtatttaag agtaccatca gatgatattt cttttactat agctccaagt
    11641 gtatccatat gtgcagatat tgttatttga tttgaatcat cttcaccagg aagagtagct
    11701 ataagtgcac ctttttttgt aagaatagtg tcaagaccta aattttcaaa atcttttttg
    11761 atttctaaaa ctgcattttc agtatatcca gctgggcttg gaatgtccaa atattttttc
    11821 attaaattta tagtattagt taaatttaaa ttcatattgt ttcctcctaa ataaatatat
    11881 ttatacaaat tataacataa atacaaatgt tctgaaaact aaaattaaaa gaatagaatc
    11941 taaacatatt taaaaaaaaa gtatataaaa taaatttgat ttatattata tagatttaaa
    12001 gtaaaaatag ttt
//
LOCUS       DAEMHW010000115        11954 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEMHW010000115 DAEMHW010000000
VERSION     DAEMHW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.115"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            370..1773
                     /locus_tag="KPK67_002552"
     CDS             370..1773
                     /locus_tag="KPK67_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2459502.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(2237..3259)
                     /locus_tag="KPK67_002553"
     CDS             complement(2237..3259)
                     /locus_tag="KPK67_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2459503.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(3294..4892)
                     /locus_tag="KPK67_002554"
     CDS             complement(3294..4892)
                     /locus_tag="KPK67_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG2459504.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGIDKIDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(4934..6199)
                     /locus_tag="KPK67_002555"
     CDS             complement(4934..6199)
                     /locus_tag="KPK67_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2459505.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGIFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGILGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6343..7740)
                     /locus_tag="KPK67_002556"
     CDS             complement(6343..7740)
                     /locus_tag="KPK67_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBG2459506.1"
                     /translation="MASTNKNNVTSKEEEKEVSTSVLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIFKGEDN
                     MSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKITHNIWE
                     EKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIWSNFGA
                     DGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDNLWIVT
                     QLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEMMLEMT
                     SRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYEVERVT
                     CNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKEEWITF
                     DGMDVLKQMYMGVEE"
     gene            complement(7778..8785)
                     /locus_tag="KPK67_002557"
     CDS             complement(7778..8785)
                     /locus_tag="KPK67_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG2459507.1"
                     /translation="MGKYQYSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQVGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            complement(8982..9272)
                     /locus_tag="KPK67_002558"
                     /pseudo
     CDS             complement(8982..9272)
                     /locus_tag="KPK67_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9772..10251
                     /locus_tag="KPK67_002559"
     CDS             9772..10251
                     /locus_tag="KPK67_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2459508.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            10248..11027
                     /locus_tag="KPK67_002560"
     CDS             10248..11027
                     /locus_tag="KPK67_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459509.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(11277..11777)
                     /locus_tag="KPK67_002561"
     CDS             complement(11277..11777)
                     /locus_tag="KPK67_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2459510.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
BASE COUNT         4281 a         2035 c         1430 g         4208 t
ORIGIN      
        1 cctactactt tgtgctacaa caatttctct atttatatga tttgtaaaat atagtacaac
       61 attttttatc attttcattt caactaaatt tgtaatattt tattcaatac ttaataaata
      121 cttaataaat acatgcttta taatacttta ctcaatatat aatcaataca taatttataa
      181 tttgtaatac tttattcaat acataagtag aaatctctat atacataaat tcattttttc
      241 atacatctta caaatttatt tttatttaga taacatgtat attttttcca tgaaaaataa
      301 ataataaaaa atagtaaata tggaaaatat aaaattgatt aacaataatt aattacctga
      361 ggtgtgaata tgaaaatgct aaacaacttt tttaagaaaa tcaacaatcc agctcctgtc
      421 tttgcacaga caaaaatgga aagagataat caaacagatg attcactttc tacaaaacca
      481 aagactacat ttagagacgt tgctggatta gacgaagtaa aagaagaact atttgaaata
      541 gtcgatttta tgaagtcacc acaaaaatat caaaaaatgg gtgcaaaaat acctaaagga
      601 gtattatttt atggtcctcc aggaacaggt aaaaccctac ttgcatctgc tgtagctggt
      661 gaaacaaact catcattttt caatgttacc ggttcagagt tcgttgaaaa atatgtaggt
      721 gtgggagcaa aaagagttag aactctattt gaaaaagcta gaaaagaagc tccaagtata
      781 atatttatag acgaaataga tgctgtagga gcaaagagac atcttgaaag taacaacgaa
      841 aaagaccaaa cactaaacca acttttagtt gaaatggatg gatttaataa agattccaat
      901 gtattaataa taggtgctac caatagactt gacttattag atgaagcttt acttagacca
      961 ggaagatttg acagacacat tcatataggt gcaccaaact atcacactag atttgaaatt
     1021 ctaaaggtac atacagatga caaacctata gacaaatctg taaatctaga acttttagct
     1081 aaaaaaactc atggtttcaa tggggctcat ctttcaaata tagcaaatga agcagctata
     1141 tttgcagtta gagatgacag tgagtgtatt acatcagaac attttgacaa ggcacttgaa
     1201 agagttattg cagggctaga atctaaaaac tctgcattgg ttgaaaaaga gaaaaaaata
     1261 gtagcatatc atgaagctgg acatgctctt gtaagtgata tagtaggaat ttgtcctata
     1321 caaaaaatat ctatagttcc tagaggacaa gcattaggtt atgttcttca attacctgat
     1381 gaagatagat atatttatac taaggatgag ttgattggaa aaataaaaat attacttgct
     1441 ggaaaggctt cagaagaatt aatatttaat cataaatcta ctggtgcaaa ggacgactta
     1501 aagaaagtca ctgaaatagc taaccaaatg gtttgtgaat atggtatgag taacttagga
     1561 tttatgacta ttgatggaaa tgataagact ttcttatgtg ataaggttca aaaggaagca
     1621 aatcgtatag ttgaaatttg ttataaagag actttagaga tgttaaagga taatcttgaa
     1681 gatttacatt ctgtttctaa attcttattt gaaaaagaaa ctatgactca tgaagagtta
     1741 aaagatttga taggaaaaga agctgtaaac taatatagtt ttaaaaattt gaggctgtct
     1801 caaaatagag ataagcctct ttttattgtt aaagttataa aaatacattt gattttatac
     1861 aaaaaagtgc ctcataaact aaaaatttca ttttgagaca cttacatatt ttaattttgt
     1921 ctatttacag aatattatat ctacataaaa ctaaactgct gtaaacttaa ctacctccag
     1981 taatgtttga agtggtataa aatctagtag tctagtttct acacttataa tttatatttt
     2041 acataactaa atttagtaga tttttatact cacaatttag aacgattgaa aacttaaaaa
     2101 tttcttatca gatatctaca acttaattag gtatctatca cttgattaaa tatctgccac
     2161 ttaattaaat atctaccact taattaaata tctaccactt aattaaatat ctatcactta
     2221 attaaatatc taccacttaa agtgtagaac ctctttcaat caattcaggt tttaacattt
     2281 caacgactga ctctggattt tcttcattta ataaggactt aagtatgtta attgtttttt
     2341 caatcaacaa atctagtggc tgtctaatcg tcgttaaatt ataactcttc catgaagcca
     2401 tttgaatatc atcatatcct atcactgata aatcctctgg caccctcaac ttgtactcaa
     2461 accttgcagc atccattaca ccaatagcta tcaagtcact tgtgcaaaaa attgctgttg
     2521 gaggatttga taatgctaac attctcttac ctgcttcata tccagaatca taatcatagt
     2581 ttgcagattc ttctttaagg ttatgaatcc cactttgtct taacttagaa taaaatccaa
     2641 ttttcttttc catttcttca cttgtttctc tcgtaaattg aatatatcct atattcttat
     2701 gacctttctt aagcagatat tcagcagcca ttcccccacc ttcaattggg tctacataga
     2761 ctgtacttat ttttatacca ggtataaaac gattaaaaag aatcacagga atattgtttt
     2821 cttcacaagt ctcagccatc accttattca tggccgaagc tgtaataatc acagcttcta
     2881 cttgaaactg aattactttt gaaattatac tatctatact ttgttgtcta ggaactttaa
     2941 aaacgagaca ctgtttacct atctcttgaa ttttttctac aaataaattg ataattctat
     3001 tgtaaaatgg acctagacta tcaccaacta tcaatcctat aacattagtt tttccactaa
     3061 tcattccccg tgctaccaaa ttaggaacat atcctatttc gtttgctaca cgaaaaattc
     3121 tttctcttac ttcaggtttt acgccctttc catttgcact accaaatgct cttgaaacag
     3181 atgattgaga tactcctgcc aatcttgcaa catcttttga ggtagccgat ttattataat
     3241 gaactttctt ttgattcatt tagtatttct ccttaaatat ctagtatttc ttcttaaaat
     3301 tgtatcaaaa ataatataat atattggata ataaaaaaag ctaatacaat tagttcttgt
     3361 ttacctaaag aaaatggcac tatagatact ccatctattt tatctatgcc ttgagaaata
     3421 aggtgatttc tcataccaaa tgcagtgtat tttttaatat catctgcatt atgatttttc
     3481 tttaaaatga cactaaaaat atatccaaat atcaatgtta ttgtagcacc tattgctgga
     3541 ttccacaacc aactcacatt atatttatta gaaataaaaa atcctaaaac taaccctaca
     3601 ataaaaccaa tagatactcc tttatcatta gctttatatg taaacattgc tagtacaaat
     3661 gcacctgtag ctggtccagc aaagtatgag atatagttac caacaaggtc aagaattgat
     3721 ttgatgcttc ctccaaatcc cataataata aaacctgtca taacaactcc tataactaca
     3781 gttataatca ttgtaacttt taatgatgat tctttgttgt cttttttgaa ataaaactca
     3841 taaatatcct tcgtaaaaac agttgtcata ctgtttaata tagagtcgat actagacatt
     3901 gctgctgcaa acacacctgc aataataatt ccaatggctc ctactggaag ttcatttaaa
     3961 ataaaagtaa tcattatttc atttgaagtt tcaaatgtcc ttcccccata aaaaacaaat
     4021 aaaattactc ctaccaatag cataaagtag taaactaaat tcataacaaa tgcactaact
     4081 acaaaagaat tttttgcaca ttttaaagat ttagatgtta aaactctttg tacttgagat
     4141 tggtcaaaac aaaagtatct tatccacatt acagttccac caattaaagt agcccaaaaa
     4201 gtatttgtca ctgaaatatc cctactgaaa tcaaatgtat ttaattttcc actctgttgt
     4261 gcaatttgta atgtatcaaa gaaacttaga ttcattcctt ttaaggctac aataattact
     4321 ataaatacag cacttattac tactatcatt tgaatactat cagtccatat aactgcctta
     4381 atcccaccta atagagtgta tataattgat acaagtacaa ctattgggac taaagcataa
     4441 agtgaccagc cagtaatcat ttgaataata agtactggaa tatataccat agaactagct
     4501 tgaatcaatg aattaataaa aaattgaata atagttaagc ctcttgaata atttcctaaa
     4561 cgaagtccca tatattcata tatagaggtt atttttaatt tatagataac tggggtggta
     4621 atgcttagtg caaaaaatat tgctaaaggt actgtaatat taaccattac tggatacatt
     4681 ccatctacat atgcccatcc aggaccacct ataaagccat ttgcactaat catagtagct
     4741 gcaactgata aagcaattgg aatccatggc attgacctac ctgccaagaa ataatcttct
     4801 tgatttttat tatcttttcc tgaataataa ccaataacta acattaatag caaatatcct
     4861 attataatac atacatcaat tgatgaaatc ataagccacc cctttctaag taacaaaata
     4921 aattcctagt attttatttt ttattattta gcgtctccgg taagaataac catacaatag
     4981 cacctgctac aaagactgcg attgatatgt aaaagatagt catcatacct aaagtaccag
     5041 aagctgtacc agctatagca ggtattgcaa acgtagatat aatccttcct ccattaaatg
     5101 acataccaga tgacaatgaa cgatatttac ttgggaacaa ctctgtgtag tatgctcccc
     5161 atgctccgga atatccaaca gcaacaccta gtaatactga tatcatatag aatgttgttg
     5221 tattatctcc acctaatttt gtatatataa aaacaaacac tgctgctaat gccataccta
     5281 taaaaggtat ttttcttcca aatttatctg caattgaacc ccagaataaa tatcctacta
     5341 attgaccaat tccttgtaaa ctatatatag acccagccac tgctgctgac attcctattc
     5401 ctgtcactag gtatgtagtt gcattatttg aaaatgctga atatgctgta aaatttgcac
     5461 ctgataaaag aatcgttccc aatccaatcc ataagtattt tcccttatac atacttatat
     5521 agctaatctt ttcaccagtt tcttttccct ttttaactaa ttcctttgta tactccaaat
     5581 catctggcac aaagaagatt gctaagatag ctccaactat aggtggtata atcgcagtat
     5641 acatcaatat ttgccaattt gcatcaccta aaatgccata tactttccca ccaaaaatca
     5701 ttgctaatga aaataaagca gttaatatag cagctaactt accacgatat ttacttggaa
     5761 acatatctgc aactaaaggc atagctgttc caaatatgcc tcccaacact actcctgtac
     5821 acaaacgtgc tattttccat aacataatat ttgtaggaga taacattggt ataaatgttg
     5881 tcaatccaat cagtaaagca acgctaatta aagtcctctt cttcccaatt tttgtagaaa
     5941 tcaccccaaa aataaatgta cctggaattg taccaaaaga cataactgca aatatttctt
     6001 ctgtttgttt taatgaaaaa ccaaatcccc catctgctaa agcataaatc attgttggtt
     6061 taataaaagt acccaccata aaattgtagc aataaaaaaa gtatattaaa gcaattgaaa
     6121 aaaatgcaat tcctcttttt aggtttgaat agtcttttcc taatatgata tcattttcta
     6181 tttcaaccgt tgttttcata tttttataat tccccctttt aaatttagtc ataattttgc
     6241 taaaagttta aacatctatt tttcatatac attccccact taaattttat aaagcaatac
     6301 ttccctatat atatttcaaa actatttata ctctaaacag ctttattctt ctactcccat
     6361 atacatttgt tttaatacat ccataccatc aaaagttatc cactcctcct ttactttacc
     6421 ttgtagtata tgataatgat tgattcccat tacttctata tgctttccag aaggctgtcc
     6481 aaaaaatcct ataccttcat ttattccacg taatctccat cttacagata catcataacc
     6541 ttcatttttt tctctttgat tacaagttac tctttccact tcataacttc catttggaat
     6601 tgatgcaagt aaacttacaa tcattccttg aatctcatca taaccattta aatctttatc
     6661 acatataaaa tgtactactg cattatcatg atagtatttt ttaacttcat ttatatattt
     6721 ataattataa atacggcttg tcatttcaag catcatttcg ccaacagaat catcttttgc
     6781 ttgatactta gttggcataa attgaccatc catagattca cagataccat aagttgattg
     6841 taaactaagc actttagatt cacttgcctt tgccatactc tttgctactt cctgagggtt
     6901 taatcctaat tgagtaacaa tccataaatt atctctaacc aaccattctt cgtggattct
     6961 attgttagtt gtggcacaat caattacagt tctaaagttt atttttctac ctgttgctgg
     7021 tccaaaatta ctatcaccta agttagttgc tgtagacaaa acacggtgag aagataaaaa
     7081 accatcagca ccaaagttag accatattac attttgacca attaatcttc tatcaggaaa
     7141 cgcatgtaat gtttgaagag tattggaaat aacatcttta ataccaacta aatttgaaga
     7201 gccacaatgc attgtaacat tattgtgata tgtatcataa ataacaccta tacctttttc
     7261 ttcccagata ttatgtgtaa tcttcataat ataatctaca aaatctctat acttagaatc
     7321 aaatccaggt atttcctgtt ccctttttgt ctgttttgaa aaatcattgt aatccattac
     7381 tccaacaggc gttacttcta atgaatctcc atagtaaatt tttgtagatg acatattatc
     7441 ttccccctta aatataactt ttttattttt ttcagtaaga gtattcgtat ttaatttatc
     7501 tgtatccact gtatctatgt taactttatt tatgtcggat ttatttgtgt ttgttttatc
     7561 tatatttcct ttatatgtgt tcgttttatt ctcatttgtt tgattttcat ttatattact
     7621 aaaattagtt tgattcattt tagctttgtt ttctttaacc gtacatgatt caagcacact
     7681 tgtagaaact tctttttctt cctctttact tgttacatta tttttgttgg tagatgccac
     7741 tttatatccc cctttgtttt agaatacgta tgcataacta agcattaaaa atacttttag
     7801 ttctctcaag aatatcaagc ccttgttgtt tcaaccaata aggaatatca attaacaccc
     7861 agttttcttg aagtttatcg cccttacgat aataaacatc aacaacttgc atatctgctt
     7921 taacctctcc acctggtaat cctaaaaaac ctccacatgg tacattagtc agattaggcc
     7981 atccaaagaa acaagcaaaa ttaccttctg caaatctaca tacatgccca ttaaaagtct
     8041 ttccttttaa attatttcta aatggtagct ggtgttgctc ttgatatcta ggtatagtat
     8101 aagaagcacc aattccacct ggaccatacc aaatcatatc tttagcccaa gatttttcaa
     8161 gtatttctgg tgggcatccc atagcaccac tttcatttaa ttcagataaa tcatctacca
     8221 ttttatttac taatgctaaa gtttttactc cttcaacttc atctgcatct tcaaaaagta
     8281 atccattatg gtcaattggc cctggatata caaaatatga acctgtttgt ggtggcaatg
     8341 gattaactcc tacttgaatc ataaacccaa ttatatctac aaataatcca gttttagcaa
     8401 ttttaccatc ttgaacacag ttaaattctg catatcttaa actaatcatt ttgcgagtat
     8461 gtttaatgcc aagccaatcc ttatcaaaaa gtcccataaa atgccccata ctcattaccc
     8521 attcactatt gtcaatctca tttgttcctg caatgaaaat atcttgtcgt ctttgcatat
     8581 tactcaatga tttttttaaa ggcttccaaa atacatctgc tacattttca gtgccttctt
     8641 gttcacgaaa tggatacaca cctttccagt tataatctga tttcatatgt tgctttaaga
     8701 cattttcaac ctcatcccca gtagcatttt ctaatgcttc aaaataattt cttacaactt
     8761 tttttgaata ctgatatttt cccataatta tctcctccca gatttattta agttataaat
     8821 taactttctc attttatgca tacgatttct tttttaatga ttttttagct gttttaagtt
     8881 tattttatga atacatatgc atttttctaa atattaacat atatctaaaa aattgtaaag
     8941 aaaatgttat cataaaaatt taatttttga cttatatcta tttaaaacaa cagaaaaggt
     9001 ttaaaacgta atattctact gggtctacca tacttgctca taagctgttc actcactacc
     9061 tcacaataac cagaatcaca caatctaagt ataatcctgt ccatagttct aatacttaca
     9121 ccacatatag tagctaattc ctttgatgta aattcagttt ttccactatt tattatttta
     9181 ttgaatactt tatatacagt attagcactt aaccctgtct ctgtagcaat ttgataaaat
     9241 cgttcatcaa agctattttt ctttttcaca gtattttgat aattatattt gtaaaattca
     9301 agtataaact ctatgtaatc atcttctata tacttttact ctgtacaata ttgtttcaca
     9361 tctataaata taatcagaca acataaaata tttttgcgtt ttttcaaaaa gttgctattt
     9421 aaagaacttc ttttaaatct gtacatgaag taaataattc aaaagcatta ttattctaac
     9481 tactcaacat taaatactta ctattttcta attactcaat ataaatactt actataaaac
     9541 taaatatata aatggcttct aaagtttaat atatctacat ggctttatat atgaggggaa
     9601 aatactagaa aaaaatatat tatagaatat ttgtatagaa taggcaaaac tttcaaactt
     9661 aaatttatca aataatcata aaaaaagtga accatctata gtaaaatttt atctaataag
     9721 tgaaaggcaa aaaagtagcg ctacgatttg catagaaggg aaggaactta gttggataaa
     9781 actacattta caaacaatat acttgaatca gaacagactt tgtatcgagt atctaagtct
     9841 attctaggaa acgaccaaga ctgtgaagat gccgtcaaca atgctatttt aaaagcatat
     9901 gaaaagttgg attctcttaa ggaagaacaa tactttaaaa catggctgat tagaatagtc
     9961 ataaatgaat gtaattcatt aagacgcaaa cgattaaagt ctctatcatt tgaagatgtc
    10021 ttcaaaaaca aaaaaattga tgaaaaagat gactatagcg atttgtatac tgctattcaa
    10081 agtctaccta aaaaaattaa aattccaata gtcctttact atattgaagg ttattcagta
    10141 gatgaagtta aggaaatatt agatatacct caaggaactg taaaaagtag attatcaaga
    10201 ggaagaagat tattaaaaac taaattagaa aacacggagg taatcatatg aataaaaata
    10261 tattaaataa ttcgtttcca gaagttccag aaagttttca taatagttta agcaaaacac
    10321 tgaatagttt accagaaaga gaggaaaatt ataaaatgat taataataaa acacacaaat
    10381 taccatttag aaaaggctta gttgcaactt tagccattac tttgatattg agcacaacag
    10441 cccttgcagt aggacatata tatagtttag ttggaaaatc aagtactaag gctacctata
    10501 catctatccc aactacagaa caagtaaaaa aagactttaa ctttacccct aatatagttt
    10561 ctgaatttag taatggatat aaatttaaag gagcatatac tgtaaataaa aaagcatttg
    10621 acaaagaaaa taatgtttta ggaaaatcaa aagcacttag ttttgaatat aaaaatggca
    10681 atgatgaaat atctcttgac acagcaaata atgtacttgg gaagagttct gacaatctaa
    10741 aagttgtaga tacttacaaa gatattaata tttcttatga agcattcgca caaaagtttg
    10801 taccagaagg ttataaaatg acagaacagg ataaaattga tgaaaaatca ggcaaataca
    10861 catttagtgt agggccaaat aatgaatcag acaaagttga agttaataaa tttaagtttt
    10921 tgacatggaa acaagatgga gtttactatt cttttacagt tcaagactca aatttatcta
    10981 tgaatgactt agttaaaatg gctcatgaag taatagatgc aaaataaaca taagtaataa
    11041 taagttgttt atgagtttat tatttatgac tttaattttt ttattagtaa acatggttgt
    11101 atcttccatc tttatatttt ctttaaattt aatatataaa attttagata tttagctacc
    11161 ttcaaaagaa gcattattaa aaagccctta aaaatatttc ttttatatgg aattttaagg
    11221 gctttaactt tattttatac aagtgtatat ggaattttaa agactttaac tttattttat
    11281 ataagtgtaa gctccatttg aattacttct tcttttacag gtctatcatt ccatgtaaaa
    11341 ccactagata ttgtatttac ctctacaaac cctaaagatt tataaacatg aatagctcta
    11401 ctattacttt taaatacatc aagctcaatt ttttcatacc catattcttt tgataaatca
    11461 ataatttgtt ttaaaagact acttccaata cctttttccc tatactcttt aagaataccc
    11521 atgcctaaat aaccaacttt ttcagtttta ggtaaaacgt cacaccaacc aatacagtta
    11581 tctgattcta aatcaattat aaacagttgt ggtaagtttt tatttataat attttttata
    11641 aattcaactg tttcttcaaa aggaaaggct tcatccatag ctagatattt cctctctttt
    11701 gctattttat caaaagtttg ccaatacgaa taaatatact tttcttctgc ctttataaat
    11761 tttatgcctt ctatcaactt aacatttccc ctttttaatt tgtgattgta gttaacttat
    11821 tttatatgat tctagtttat aatgctatat aaattattat atattagctt aaaatatctt
    11881 tgtatatttc tataaaatta aaaccaatat atctatctct tgtatctcta ttaaaataat
    11941 ttttaatagt ataa
//
LOCUS       DAEMHW010000116        11804 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEMHW010000116 DAEMHW010000000
VERSION     DAEMHW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.116"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    85..316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            424..1905
                     /locus_tag="KPK67_002562"
     CDS             424..1905
                     /locus_tag="KPK67_002562"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBG2459511.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            1915..2808
                     /locus_tag="KPK67_002563"
     CDS             1915..2808
                     /locus_tag="KPK67_002563"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBG2459512.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            2932..3993
                     /locus_tag="KPK67_002564"
     CDS             2932..3993
                     /locus_tag="KPK67_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBG2459513.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4148..4660)
                     /locus_tag="KPK67_002565"
     CDS             complement(4148..4660)
                     /locus_tag="KPK67_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBG2459514.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            5180..6115
                     /locus_tag="KPK67_002566"
     CDS             5180..6115
                     /locus_tag="KPK67_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBG2459515.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(6207..8078)
                     /locus_tag="KPK67_002567"
     CDS             complement(6207..8078)
                     /locus_tag="KPK67_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBG2459516.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKIAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            8268..9194
                     /locus_tag="KPK67_002568"
     CDS             8268..9194
                     /locus_tag="KPK67_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG2459517.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILRGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9333..9479
                     /locus_tag="KPK67_002569"
     CDS             9333..9479
                     /locus_tag="KPK67_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459518.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(9584..10108)
                     /locus_tag="KPK67_002570"
     CDS             complement(9584..10108)
                     /locus_tag="KPK67_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2459519.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVTENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDAYIMGRYNKNLI"
     gene            complement(10161..10385)
                     /locus_tag="KPK67_002571"
     CDS             complement(10161..10385)
                     /locus_tag="KPK67_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459520.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            10582..11706
                     /locus_tag="KPK67_002572"
     CDS             10582..11706
                     /locus_tag="KPK67_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBG2459521.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPSVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
BASE COUNT         4174 a         1667 c         1515 g         4448 t
ORIGIN      
        1 aaaaaatatt ttttccaaat ttttatatta atacttgact ttgtctgttt tataaactag
       61 aataattaat aatattaaga atattgaaag gcattgatta ggaagagtaa atctgaatcg
      121 attaccagag aaggaaacta ctagctgaaa tgtttcccta attagaagga ttgaaaacta
      181 cctaggagct tctatccgaa gttatcttaa tgattttagt aggaatagac gtttttatta
      241 cttgcgttaa agtatcaagt ggatttttta aaatcaattt gggtggaacc gcgggaaaca
      301 gattctcgtc ccttttttag ggatgagggt ctttttttat tacctaaatt cagtttttaa
      361 aatattctgt atctaattta attagctaat taaaaattta aaatatatgg aggattataa
      421 aaaatgtatt attcaagtac gagaggcacc gaagaaaaag taactgcatc acaagcaata
      481 ataaaaggaa tatcaagtga tggtggtctt tatgttccaa gtgaatttcc caatgtaaaa
      541 aatgaactta taaatttagt aaatttaacg tattctcaga tagcattttt tgtacttagt
      601 aaatttttat gtgattttac tgaagatgaa attaaaaatt gtatagaaaa tgcatatgat
      661 gaaaagtttg attgtagtag tatagctcca ttaaataaag taaatgatac ttatttctta
      721 gaactatatc acggtccaac tttagcattt aaagatatgg cacttactat aatgcctcat
      781 cttcttaaaa catctataaa gaaagataat ttagagaaag atgttgtgat tcttactgct
      841 acatctggag atacaggcaa agctgcactt gagggattta aagatataga taaaataaag
      901 ataattgtat tcttcccaga agatggtgta agcccagtac aaaaacttca aatgaaaact
      961 caaactggaa aaaatacata tgtagttggt ataaaaggaa attttgatga tgcacaatca
     1021 ggtgtaaaaa agattttttc tgataaagaa tttaatcaaa aactattgga taataattat
     1081 atattatctt ctgcaaattc aattaatata ggaagacttc ttccacaagt tgcatactat
     1141 ttttattcat atatgaaatt agttagcaat agtgagataa atttaaatga taaaataaat
     1201 tttgttgtcc caactggtaa ttttggtaat attctagctg gatattatgc caaacaaatg
     1261 ggtcttccaa taaacaaatt gatatgtgca tcaaatgata ataatgttct ttatgatttc
     1321 tttaaaactg gtgtatatga taaaaataga acattgaaat taactacttc tccttctatg
     1381 gatatattaa tttcaagtaa tttagaacga ttactgtttg aaatatcaaa tagaaatact
     1441 gatattgtaa ataagctgtt atcagacctt agtgataaag gagtctacaa aataagtgat
     1501 gatatggaaa aaaatttagc ctcattctat ggagaatatt caaatgaaga agaagttaaa
     1561 agcacgataa acaatgtgtt taaaaattat aattatttga tagatactca tactgctgtt
     1621 gcatacaatt gttatgaaaa atataaaaaa gaaacttctg ataacacaaa aactgttatt
     1681 gtaagtactg caagtccatt caagttttct gaagatgttc taaagtctat agattgtgat
     1741 tttaaagacc ttgatgactt tgctataatt gatagattat caagtatttc taaaatagat
     1801 attccaaaac ctattaaaaa tcttaaaaat gcagaaatac tacataaaga tatttatgaa
     1861 aaagatgaat taaaactagc tataaaaaaa ttcttaaagg tataggtgat tattatgtta
     1921 gagattatag ttcctgcaac aagtgctaat ataggtccag gatttgattg tcttggaatt
     1981 gcgttaaata tatacaataa attttatgtt gaagaaattg aaagtggtct tgaaatagaa
     2041 ggttgtgaag atgcttacaa aaacgaaaac aacttagttt atacatctat gaagtacttt
     2101 tttgatagag ttaaaccaga aaaaatacct gctggtatca aaataaaaat ccaaagtgaa
     2161 gtacctatat gtagaggtct tggcagtagt gcctcttgta tagtagctgg agttatagct
     2221 gcaaatgctc tatcaggtgc taatttagat aaaaatcaat tactaaatat agcttcagaa
     2281 atagaaggtc acccagataa tgttgctcct gctattttag gaaatatgat tgtatctgtt
     2341 actgataatg agaacataca ttatgatatt ataaaaattc ctgaagaact taaattttgt
     2401 gcaatgatac ctaattttaa gttatcaaca gaaaaagcta gaggtgtttt accaaaggaa
     2461 ataccatatt ctgatggggt attcaatgtt agtagagttg ctttactaat atctgcctta
     2521 ctaaacaaaa actttgacct acttaaagta gcttgtcaag acaaattgca tcaagattat
     2581 agaggaactc taattgagaa ctacaatgat attgttgaaa aatctgaaca attaaatagt
     2641 attggagtat ttttaagtgg agctggacct actataatgt ctttaataaa agaaaatgat
     2701 gatagttttg tggataatat gaaaaattac ctacaaaaac taaaatctga ttgggaaata
     2761 aaagagttat gttgcgattc taatggagct gttttaaata taatataatt atcgtcttct
     2821 atacaaaata taactactct tttattataa tttgtagtat aataattagg actaggtaat
     2881 aatattaagt attataatct agttctttaa acatgtttac attgaggagg gattatcata
     2941 aaagagttaa taattagaga tttagttgca aaagttccta taattcaagg tggaatgggc
     3001 gttggtatat ctaggtcatc actagctggt gcagttgcta agcttggtgg tgttggtgta
     3061 atttctggag ttcaaattgg atatgatgag gaagattttg aaactaatac tataaatgca
     3121 aatttaagag ctataaaaaa acatatttct aaggccaaag aaatctcaaa tggtggtatt
     3181 attggtatta attttatggt agctatgaaa gaatatgaaa catacgtaaa ggaagctgtt
     3241 aaagctggtg tagaccttat aatatctgga gctggattac ctaataagct accatcatta
     3301 gtaaagggaa gtaatgtaaa aatagcaccc atagtttcaa ctgcaaaagc tgctaatgta
     3361 attttaaaaa tgtgggatag aaaagagaaa actactgctg acttaatagt tgttgaaggt
     3421 ccaaaagctg gtggtcacct tggatactct aacgaggaac ttgataacat agattctata
     3481 gactatgata aggaatttgt tgaaatttta aaggttgcaa atacttatgg agagaaattt
     3541 ggtagaaaca taccagtagt tgctgctggt ggtataactt ctagtagtga tgttaaaaaa
     3601 tatatagata tgggtgcaag tggtgtacaa gttggtacaa gatttgttgc tacttacgaa
     3661 tgtgatgccc atgagaactt taaaatggca tacataaatg ctagtgaaga agatgttcat
     3721 atagttaaaa gtcctgtagg tcttcctgga cgcgccataa gaaacaaatt tatagaagaa
     3781 gttaaaataa atcgtccaga aataaagaag tgctataatt gtttgattcc ttgtaatcca
     3841 aaagaaactc cttactgtat ttctcaagct ctaatcaatg cagttaaagg agatgttgaa
     3901 aattcacttt tattttgtgg aaatgatgcc tataaaattg ataaattaag tactgttgaa
     3961 gatgttatca atgaattgat ttctgaatta taatttaaag aaaactaaat taaaatttta
     4021 tcaagctaag gagtttaaat acgttattta ttaatcttta taaacatatt tatgtcctta
     4081 gcttttttgt gtcaatttcc ttttattgaa attttaattt aattagagat agtattaacc
     4141 cttataacta atcttctttt acaagtacat tggttatttc agctatatat aaagtatgat
     4201 aatctttttg tggataccat cttccatcta taccttcagc aataaaacat tggggttcaa
     4261 gtttctgact atacattttt ttgcatataa tagccatttt agcttcctta aaataaggtg
     4321 tatcatttaa gtgttctata ttaaatccaa tctgtgaaat cttatcctct tctctaccag
     4381 atacttttcc acaatatgaa agctctttac gaaaatcttc atcaaaaaat gtaagagaaa
     4441 atttatctgt attatctata aattccttag tataacgctg tggtcttatt actacaaaag
     4501 caacatcttt tccccacata actccaagcc ctccccagct agcagtcatt gtattcactt
     4561 taccttcatt ttctgctgtt ataagtgtcc aatctttacc tatcaatcca aatggattat
     4621 ttttaatctc ttctgccaaa atttctttaa aatcactcat atctctaata acctctcatt
     4681 ctcttatatt tatattcctt ttattttcgt gataaattat aaatgaagtc tagttttgaa
     4741 aataaaatac ttttttaaat atattattgc aaataagtaa atatatatat acactcttat
     4801 tttttaatat atttaataat ttcactcaga atatatatat tctttactca aattaaatat
     4861 aacttatata ctttatgtgt aaattatggt aaaaatatta tttaatcaaa tcaaaacttg
     4921 atttatatac ctggaaaagg tattatatta aagagtgctt aaaatacgca tttcataaat
     4981 agctatatat aaaataataa ataaggagag tgatgggcaa tggatactag tccgttgcgg
     5041 agagaatttt ttacattttc atcacttaat aatatctaaa atcaaaacta atacatatat
     5101 tcagttatat aaattagtta attaatagat actattaaat gatgagattt atataaaact
     5161 gaataaagga tgtgttgcca tgataagata ttacaaaact atagattcaa aattagagaa
     5221 acttagtttt tttgaagatg gctgttggat aaatcttgtt gaacctaatc atagtgaaat
     5281 caacgaaatt tctaatttat taaatataga tgtagaaagc atagaatctg cactagatga
     5341 agaagaacgc tcaagaatag atgttgaaga caatcatact cttatactta tagatatacc
     5401 agtagacgaa agtgactcta attcttctca ttatactaca atacccttag gtattatact
     5461 aactgaagaa gctattgtaa cagtttgtga tgcacaaact aagattttaa atgactttat
     5521 agttggtcat attaaagatt tttttacgtt taaaaagact cgctttttgc ttcaaattct
     5581 tcacaaaaat gcagcttatt acttgcatta cttaagaaaa ataaataaaa tgacaatcat
     5641 aatagagcga gaaatttaca aatcaatgaa aaacaaagaa ttagttcaat tattggaatt
     5701 agaaaaatct ttggtatact tctctacctc actaaaatct aatgaacttg ttttaaacaa
     5761 aatggttaga actgctggta taaagaagta tccagatgat gaagacctat tagaagatgt
     5821 tatagttgaa aatagacagg ctcttgatat ggctaaaata tatggagata tattgagtag
     5881 aattatggat gctttcagtg caattataag taataatcag aataatgtta tgcaaatttt
     5941 aacagtagtt acactaattt tctcaatacc tacaataata tcaggattct ttggtatgaa
     6001 tgtcatcaac atgccatttt ctaataaccc aaacggattt tggataatac ttcttattac
     6061 ctctataata tgtatagtga ttacattttt catgtcaaga aataagctat tataaaatat
     6121 taataaaaaa gccattagaa ttaattgatt tacatttatc ctaatggctt ttatatattt
     6181 aaatctatat aatcttataa tactttttaa ctatcatgtt cttcatctat aatcatttca
     6241 gtttcaagcc cttctagcat tgaatctaaa ctttctttta tatttagtat ataaattggt
     6301 acatactctt cttcatctat tacctcttct tgcaacttaa atggaggaat taatacttca
     6361 tcaaagtcta tatttaaatt aggaaagcct tctactatct cttcacaatt atcgtactct
     6421 tcatttgcta aagaaatgta catatctagg ctctttttat aataaagacc tgcaagcata
     6481 tactcacact tcttacttcc atatttaata ccttctttat aatatttctc agcttctgcg
     6541 tcatcattta attttaaata aatttgacct aaattatata ttgattcaac acaaccttgt
     6601 gctctagcct tttcatacca aaatttagaa tttacataat ctttatagaa catatcataa
     6661 attaatccta ccatatgttg agcatacaca ttattttcat tggcaggtgt atcaaacatc
     6721 attttagact cttccaattt accttctcta aaataaattc tacctagatg tatacgagca
     6781 ttcatatggt ttttgctact tgccatttca aaatactttc tagcattttt tgtatttcct
     6841 aagctctcat atatacaccc caatctataa ctagacctcg tatctccatt ttcagatgct
     6901 ttcttatacc aacttattgc ttcttcaata ttatttaaat cttcatagat atttcctaat
     6961 ttgatttgac aagatacatt atttggaatc ctcgaataat acgaaatcgc tttttctata
     7021 tcttgattct gataatataa atcccctaga ttttcaaggg aacttttgca tccaacagct
     7081 atagcatcct cataatattt aatagcattt tcataatctt tttcttcaaa atatatacct
     7141 gccagattat tttttgcttc tgttatatca tcttcagcag ccattttata ataagttttt
     7201 gccttctcta tctcattttc tctattatat aaattagcaa gtctatactt agaaagtgta
     7261 tgattttttt ctattgcttt ctcataataa taaattgcat ctcccagctc ttcttttcta
     7321 tcatatacat atgcaagcct atactcagac ttcacatcat ctctatcagc actcttttta
     7381 taatacttct ctgcatcttc taaattatca tttatttcca taatcatacc caagttaaag
     7441 caacctttca tatgacctga cttgtacaac tgtttatata aacttactgt tgatttatta
     7501 tctttctttt ttccatttaa tattgctaaa ttatattttg catcatcatc taaaagcaca
     7561 gaaccttttt tatagtattc atacgcttca tcatctttct ccaaaaaagt atataatacc
     7621 cctaaattaa aacaagcttt acctaaattc ttgtctgctg ctatttttaa ccatttttca
     7681 caccttacaa aatctttcat tttaaaatgg catacaccta aattattttg tgcttgtaaa
     7741 taacctaaat ttgctatctt ttcataccaa tatatagcat attcatagcc atctaactta
     7801 tagtacatat tacctaaatc aaaagcagca tcttcacttc cacttttata tgccattgaa
     7861 taccactttt ctgcttcttt aaattgtttt ttactttcat atatatttcc taaagtatat
     7921 tgagatttta cgtaattatc caatgcagca ctcttataca actctattgc gctttccatc
     7981 ttacccatct tttcaaggag ggcaccctta gtatgtacga ctttcgggtc attagatata
     8041 ttttttattt tttttataga aaattttgta tttttcatta ttcgctacct acccatttta
     8101 taaaatatat tctctcaaac attattatac attataatct acaattaatg ctataatatt
     8161 gaatttttac cttttgtcac tatattgtaa cattaaattt taaattttat aatcattaga
     8221 ttttattatg taattggtat ataatgtata tatagggggg acattttatg aatttcagca
     8281 atatttttat ccaagttgca gttttattta taattatttt agttggatat tttgtcagaa
     8341 aattcaactt attagatgac cactgcacat ctaagctttc aactttgaca atgacagttt
     8401 ttttaccatc aatgataatt agctccatgc aaattaactt tgatagtaaa atgatacaaa
     8461 aaatactatt gttattattt acatctttga ttatgtatat agtttcaatt ataattgcat
     8521 ttttattgaa atacatttta aaatgtaatg ataaaaaaga tttgggaata tatcagtata
     8581 tagttgtttt ttcaaatgtt gcttttatgg gttatcctgt aattgaagca gttttaggtc
     8641 atgaagcaat attctatact gcaatattca atttgccttt taacttattt tctttcactt
     8701 taggaattta tcttttatct aaaggcagta caagtagagg tttttcaata aaatcactta
     8761 taagccctgc tacaatagct attgttattg gacttttttt atttgtaaca ggacttaggt
     8821 taccccaatt tataaacgag ccacttgaga tgttgggaag tataactaca ccaatatcca
     8881 tgattatcat tggaagctta cttgctaatt cttcagctct cgattgtttt gtcaacaaaa
     8941 aattatatat agtcactttt ataagattat tggtacttcc tgtgattgtg tattttattc
     9001 taagaggatg gataaatgat aagatgattt tagctatacc agtagtaatt tcttcaatgc
     9061 ctgctgctgc aaatactgct ataatggcta atcaatatga ctccaatata acactagctt
     9121 cacaatgtgt gttttttaca acactatttt cagttatatc tatacctttt ataagtattt
     9181 tactcttatc ttgatttatt tttttatgta ttatataata acatattcaa taaattacaa
     9241 atataaacat tttattgtag tatattgatg gaattttttc ctagatattt gtcaataata
     9301 tctatatgta ttatagaata ggaggcttaa atatggatat ggatttactc tttaaaatag
     9361 tatttgctat agttgctata ggtgtttctg taaaaatcat taaaactgtt gctagtttag
     9421 cttttaaaat agcgttgata ttgctaataa ttttatttgt atatagaatg tttttgtaaa
     9481 catttaaaaa taagtttttt aatacaatag agaagtaaaa tagattatct ttaatataaa
     9541 ttttttatta aagacactct attttacttc tctattagct tctttaaatt aaatttttat
     9601 tatatcttcc cataatataa gcatcttcaa attttccatc tctgattaca gcatattttt
     9661 tagttccctc tatttcaaaa cctagctttt tatataaatt taatcccttt tcattatcca
     9721 caattacttc taatcctact ctagttaaca taatccaatt atctgctata tctaaaagtt
     9781 gttccataag cttcttacct actcctcttc cctgataatt ctctgtaaca cttattccta
     9841 tttctgcgct atgtctcaat cgtggagaag aatttacatg taacccagca aaacctatta
     9901 catctttttc tccattatta ttagtttcag ctacaaacat ataatcattt tctcccaaag
     9961 aagctaacat agcttcagtt ttatcgactc tgtcactcat taatgccaaa gtatttctca
    10021 taacactgta tgttcttcta atttcattaa aagcttgtgc atcttctctt cttataggtc
    10081 ttattgttat atctataggc ttactcatat tggattcccc ctcaaacata atctttattt
    10141 ttaattaaaa ttttacatct ttattttata ctgttcaaat ctatgtatgc ttctacctga
    10201 ggtacatata tcatattatc ttttatatgt gtacttccaa attcttttaa ttctgtacct
    10261 tctttatcct tagttatttt atatctaatg gattccatac cttcaactac ttcagcatga
    10321 aaaaacatac cttcatattg tttaaatcta aaattaaaat tgtctttagt aagttttatt
    10381 atcatatttt tatctactcc ttatataatt gatatttttc ttattattag ttgtatatat
    10441 actgcttatt catattaata tattataact tatcatttac ttagtataaa gttataaata
    10501 gatatattta tacttatttt acaatctttc ttcaattgat atataatatg tttatataaa
    10561 ctaatgtatg tgaggtgaaa tatgagcaaa aaaacaaaat atttttcttt aattctcttt
    10621 cctattgctt tgcttttaaa ttttatagcc agcaaagttc caaatattgt tgaaaaatat
    10681 tactcccaat tcatagataa aataattgta caaatattga gtaaagtttc tggaattttc
    10741 ccattttctc tctatgagat tacaatgtac ataatagtta tatctatctt cttattttta
    10801 tgttgtactt tatctacaat ttttaataag aaaaaaaaat taaacatata tttaaaaaat
    10861 tccattttaa atatattatc cattgtatca atagtctact ttctttttgt agtgctttgg
    10921 ggaataaact ataataggat tccactagaa accacactta taaataacta taatttaaaa
    10981 aacaatacct ctatccaatc aaaacaatat agtgttaaag agcttactaa attatataaa
    11041 ttccttgtaa ttaaagcaaa tgaaactaga aaactcacat tacaagataa aaatggtatt
    11101 gtaaaatcta atacagatta taatggtgtc ataaatagag ctcaactagg atacgataat
    11161 atcttggata tattacctag tgttagtggt agttattcta agcctaagta tattatttca
    11221 tcaaatctaa tgtgctatac aggaataact ggtatatact ttccttttac aggtgaggca
    11281 aatgtaaata tagcaatacc tgacttatat attccttgta cagttgaaca tgaaatggca
    11341 catcaaaggg gatttgctag tgaagatgaa gctaatttta tagcgtatct tacttctata
    11401 aaacacccca atattgattt taactattct ggatatattt tagctttaaa ttatactgct
    11461 tctgctttaa gtaaggttga ttataatgca tatgttgata tttctgctgg catttcagat
    11521 tctgtccgta gagatttaaa aaatgaaagt gaattttggc aaaaatatga aggtaaaatt
    11581 aatgaaattt ctaatgagtt taacaattct tacttaaaag ctaatggcgt tactgaaggt
    11641 actcagagtt atggtaaaat ggttgattta ttgctaactt attatgaatt atatccatat
    11701 aactaaacac tacaattcaa aattttattc atatacagct gatattaaat cagctgtttt
    11761 ctatttaaat aaattattta acttcaagca atcttatata attt
//
LOCUS       DAEMHW010000117        11679 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEMHW010000117 DAEMHW010000000
VERSION     DAEMHW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11679
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.117"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1..3508
                     /locus_tag="KPK67_002573"
                     /pseudo
     CDS             1..3508
                     /locus_tag="KPK67_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            3693..5627
                     /locus_tag="KPK67_002574"
     CDS             3693..5627
                     /locus_tag="KPK67_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBG2459522.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5733..6728
                     /locus_tag="KPK67_002575"
     CDS             5733..6728
                     /locus_tag="KPK67_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2459523.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6700..7485
                     /locus_tag="KPK67_002576"
     CDS             6700..7485
                     /locus_tag="KPK67_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG2459524.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVSQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7473..8171
                     /locus_tag="KPK67_002577"
     CDS             7473..8171
                     /locus_tag="KPK67_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459525.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8294..8767
                     /gene="ybaK"
                     /locus_tag="KPK67_002578"
     CDS             8294..8767
                     /gene="ybaK"
                     /locus_tag="KPK67_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBG2459526.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            8964..9803
                     /locus_tag="KPK67_002579"
     CDS             8964..9803
                     /locus_tag="KPK67_002579"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBG2459527.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            9816..10364
                     /locus_tag="KPK67_002580"
     CDS             9816..10364
                     /locus_tag="KPK67_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBG2459528.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10430..11611
                     /locus_tag="KPK67_002581"
     CDS             10430..11611
                     /locus_tag="KPK67_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBG2459529.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEDRLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
BASE COUNT         4719 a         1177 c         2146 g         3637 t
ORIGIN      
        1 atcttgttta ttattataat tattttaaca tgtggagctg ttttcaagtt taaatttaaa
       61 ttagataata aattaaaaga aaccacacat acatatttgt ctgagtttac agaacacaat
      121 ataattaatc tcaagacaag acttagtggt caatttgaca tgcttgaatc tgttgctaac
      181 ttcataggaa acttgaataa tataaataat gaaatggtga ttgatttaat ggattctgat
      241 gtaaagcgaa gttctttaat tagaatgact atctctacat tagatgggga atgttattct
      301 aatgatggaa ttacccataa tgttcaagat agagattatt ttaaaaaagc taaaaatgga
      361 gaagaatata tcagagcctt taaaatctat tgttaataat aaagaaataa tagttttatc
      421 taccccagtt tataaaagtg ggaaaatagt tggtgtttta agtggaattt atgattcaaa
      481 aaagttaaat gagttattag gattatctgc atttgatgaa aaagcagatg tttttgttgc
      541 aaagaaaaat ggagatatag ttgctcaaaa aacaaataaa aatagaaaga cgctatcatg
      601 tatatttcag ctttttgaca aagataattg tgatgaagat gattgtctaa aaactataaa
      661 agaaaatatg aaaaatgata agtctggata ttttaattat aacttagata atgaaaaatt
      721 tatgttaaac tatcagccac ttggagttaa tgattggtat gttttttctg ttgtgccaaa
      781 taaaatagtg tctattcagt cagagaatat atcttatgat gtatatattt ttgcattaga
      841 aatactacta gttatattga tttttataat atatatagca tgtattataa ataataacac
      901 taaaattatt ataaatgaaa aagaaaaatt taaagtactc actgataata tactaggagg
      961 ggttaaaacc tgtttaaaag atgaatatat gactatgaca tatgtaagtc aggggttttt
     1021 agagcttaca ggttactctt gtgaagattt aaataaaata tttaaaaata gatttaaaga
     1081 tatgatttat aaagatgatt tagacaagtt taattgtatt attctaaatc aattagaaaa
     1141 acaagataaa attgaagttg aatatagaat tgtaagaaaa gatggaagta taatttggat
     1201 tttggagaga agtaagtcta taaatgataa taaaggaagt gagtgtattc agggagtttt
     1261 aactgatata acagaactta agaaaataca agaagaatta gattcaaaaa gatttgaaat
     1321 acaggctatc aatgatagta ttgttggtgg aatagtgata actgagataa atgatgattt
     1381 tgatagtata tatttaaatg aaggttattt aaatatgatt ggatatacac gcaaacaact
     1441 ggaagatgag ctaaataata aattaatcaa acttattttt aaagatgata gaacagaatt
     1501 aattaatagt ataaacaaac aactaaaatt aagtgataca tttgcatctg aacaaagagt
     1561 tgaaaaaagg gatggtacta ttatatggat tttattaaaa ggaagaaaga tgatttatga
     1621 taatggaaaa tctgtacttt gttgtgttat tgtagatatt acttctgcta aattattaga
     1681 gcaagaactt aaaaagagtg aagaacgtca tgaaatcatt ctaaagcaga cgcaaaatat
     1741 tataatagaa tgggatatag aaaaagataa aatgaatata tcacctaatt ggtataataa
     1801 gtttaaatat gaccctataa ctgaaaatgc aacaaaaaat ataattaaat cagtagatat
     1861 atatcatgat gatgtggata agtttatttc attaattaaa cttacttcac aaggtagtga
     1921 atatgaagag tgtgaaatta gacttagaac tagagatact atttacgctt ggtttagtat
     1981 aaaggcaaca actttatttg ataataatgg taaaccttat aaagtagtag gaagtttgac
     2041 tgatattgat gaatcaaaac gagaagctga aaagttaaaa gaaagagcag aaaaagaccc
     2101 tcttacagat ttgtataata aaaaagcatc acaatcactt atagaagagt acataacatt
     2161 ggataaaaaa agaaaaggtg ctttaatgat tattgatatt gatgacttta aaggaataaa
     2221 tgataattta ggacatttat atggggatgc tgtattaagt gaaattgcaa atgacctaat
     2281 aaatctattt agagcaaccg atatagtagg cagaattggt ggagatgagt ttattgtatt
     2341 tataaaagat atttctgaaa caagagatat tataaataaa gcagaagaat tggtaaaaac
     2401 atttgaaagg tcttttttag gtaaaggacg aaattataaa atatcattaa gtgtaggaat
     2461 tgctaagttt ccacaagatg gtatggacta tatgacacta tttagaaatg cggatatagc
     2521 tctttactca gccaaaggga agggcaagaa ttgttataca ttgtatgata aaaaaattga
     2581 taaagtccaa tatataacta atacaagaaa tgcaggagat agtgataatg tagagatgtc
     2641 ttttaaagaa catatcacaa attatatatt tgatatacta tatgaatcaa gtgatataga
     2701 agtggcagta aatttaattc taagtattgt aggtaaacac tttaatgtga gtcgcatgta
     2761 tatatttgaa aattcagatg ataatcgagt atgtaaaaat acatttgaat ggtgtaacga
     2821 aggtataacc tcggaaatta ataatcttaa aaatatttgt tacaaggatt taggcaacta
     2881 tcttttaaat tttaatgcag atgggttatt ttattgtaat gatattgaaa atttaggtag
     2941 tgatgtgaaa aaagtactta agccacaagg catacgttca atattacaat gtaaaatttt
     3001 agagggcaat gaatttagag ggttcatagg atttgatgaa tgttcatata atcgtatgtg
     3061 gactaatgat gaagtgaaca ctttgacatt tatatctaaa acgctcagta tgtttttatt
     3121 aaaaatgcgt attcagagta agctagaaga gtctttaaca attcaaaaat ctgtgatgga
     3181 taatatggat atatgggcat atgttgttga caggaatacc tatgaattac tatttataaa
     3241 taaaaaaatg cttgagtcag caccaaacac tcatgtaggt gatttatgtt ataaagcgat
     3301 atggaataat caagataaac cttgtaaaaa atgccctatg atatatttaa aagatggtga
     3361 tgatacttat aaatcattca tgcataataa aaaatttgag gtacaggcaa atataactac
     3421 aactactttg aaatggtttg atggtaaaga tgcatgttta atatttggaa gtgaagtaac
     3481 tagtattgaa cctaaagaaa atacttaaaa tactgtgtta tatatatact taaaatcgca
     3541 atttgaattt attagactta aaatgtctat ataaatcatt taagttgtgg ttttaagtat
     3601 tttttatgat ataattaagc gttaagaaca aaattaaaag atacttttgt ataaaaaaat
     3661 atttgttaaa tatatattga gcaggtgata atatggaaaa taagttttta ccaataagca
     3721 aacaagatat gatagataga ggatgggaag aattagattt tgttctagtt acaggagatg
     3781 cttatgtgga ccatcatagt tttggaacag caataatatc tagagtttta gaaaatgctg
     3841 gatataaagt tggaataata gctcaaccag actggagaac tacagatgat tttatgaagc
     3901 taggaaaacc tagacttgca tttctagtta atgcaggaaa tatggattca atggtcaatc
     3961 attattctgt aagtaaaaag catagagaaa aggatatgta ttctccaggt gggaaaatgg
     4021 gttatagacc agatagagct actatagtat attgcaataa aataagagaa gcatacagtg
     4081 atgttgcaat agtaataggt ggaattgaag cgagtttaag aagatttgca cactatgatt
     4141 attggagtga taaggttaga aagtctatgt taatagatag tggagcagat ttattagttt
     4201 atggtatgag tgaaaagcag attgtagaag tagccaatgc acttaatgat ggatatgacc
     4261 caaaatacat tagacatatt gatgggacat gctatatttc agatacattg gaagaaatat
     4321 atgataaata tatattaatt ccatcatata aagaaatttg tgaagataaa atgaagtatg
     4381 tagaagcttt taaaattcaa tatgatgagc aagacccatt tagaggtaat ataattgttc
     4441 aacctcatgg aagtaagtat ctggttcaaa ataagccaga aaaaccactt agtagagaag
     4501 aactagatga agtatatgga ttaccatatc agaagacata ccatcctgtg tatgaaaaat
     4561 ttggaggaat accagctata gaagaagtaa aatttagtat agtaagttca agaggctgtt
     4621 ttggtagttg ttcattttgt gcaataacat ttcatcaagg tagagcagtg caaagtagaa
     4681 gtgaaaaatc aattattgat gaagcaatag gaataacaaa tttagatgat tttaaagggt
     4741 atatacatga tgttggaggt ccaacagcta attttagaag accagcatgt aaaaaacaaa
     4801 ttactaaagg tgcttgtaaa aatagacaat gcttatctcc atcaccatgt aaaaatttag
     4861 atgcagacca cagtgaatac ttacatttac ttagagctgt aagaaaatta cctaaaataa
     4921 aaaaggtgtt tgtgcgttct ggaattagat acgattatgt aatggcagat aagaacaata
     4981 aatttcttag agagttgata gagcatcatg taagtggtca gttaaaggta gccccagaac
     5041 atatatcaga agaagtttta aagtatatgc aaaaaccagc aggaaaaact tatgataaat
     5101 ttagacagaa gttttttgct ataaatgaag aattaggtaa aaagcaatat ttgataccct
     5161 atttaatgtc tagtcaccct ggttctacct taaatagcgc tatagagtta gcagagtatt
     5221 taagagatac tcactaccaa ccagagcagg ttcaggattt ttatccaact ccagggactc
     5281 tatctacaac catgttttat acaggtattg acccgcttac aatgaagcca gtttatgttc
     5341 ctaaatcaaa acgtgataag gcaatgcaaa gggctttatt acaatataga gcacctagaa
     5401 attatgattt ggtatactct gcgttagttg aagcaggaag agaagactta attggttttg
     5461 gacatagatg tcttataaag ccaaaaaatg aaaagcctta ttttaataga aataattcta
     5521 aaaaaaatgt atctaaagga acaaataaaa acaaaaaaac taatacaaat aacagaagta
     5581 ataaaaatca acaaaaaagt tcaactaaaa aaaagaaaaa acattaaagt ataaataaaa
     5641 atacttaaaa aagaaggatt attcgataaa ttaacagaaa ttagtataaa taaatataat
     5701 tattttaatg acgttacctt tgggaggaag agatgaaaaa gtatatttct ataatattat
     5761 tagtggtgtt gactatggtt gttttggtag gatgttcacc aggaaaagat aaccctaaaa
     5821 ataaggagct atcagttgtc aaacaagtaa aagtagctgt tccagatgga ctaccagcag
     5881 tggcaatagc taaacttgca aatgaaaatc cagagattaa agaaggatat gaaacagtgt
     5941 atagcattga aaagacacct gaggcgattt ccactagagt gatgaaaaaa gatgcagata
     6001 tagctatagt tccttcaaac atggctgcaa tagcgtataa taaaacttca tcatataata
     6061 ttgttggaac tgtaggaatg ggttcacttt atttggtgtc aacagaaaat ataaaagatt
     6121 actctgattt agttggtaaa gaagttggat gtactggaaa aggacttact ccagacataa
     6181 ctataaaaag tcttttgtta caaaaggata taaattattc agatataaaa tttaattatg
     6241 taaattcagc aagtgaactg gtaccattat tggctactgg taaagttaaa acaggtattg
     6301 ttccagaacc tgcacttagt gctttgatgt ctaaaaatcc agacataaaa attataaaaa
     6361 gtttaaatga ctcatggaag gaagtaagtg gttcaaaaga tggttatcct caatctactt
     6421 taattgtaaa gtctgaattt ttaagagata ataaagattt tgttgattct tttgtaggtc
     6481 aattatcaaa tagtatagat tgggcaaaca aaaatcctga agaattaggt gcatattctg
     6541 aaaaaataaa aatatctact gaatcaaaga taataggaaa atctcttgaa agagctaatt
     6601 tgaaatatat tccagttaag gacatgatta aagattataa aaattactat gagaaattag
     6661 ctaattttga tgataagaca ttaggaggca aagtaccaga tgaagcaata tactttgttg
     6721 agaaataaaa aaaataaaga acaatttata gataaaatgg aggtaacact ttcttgtgtt
     6781 atcctcttat ttttatggca aattattgct ttaaaaattg ataatgatat ttttttacca
     6841 acagtaagtc aagtatttaa tagtataaaa gagattatgc taaattctag tttctatata
     6901 gatatacttt actctatggg gagatgtttt tttagttttt tactggctat gatttttgct
     6961 attatacttt caatagtgtc ttatttaaat agatttttta gaaacttatt aaagccaatc
     7021 aatgctttga cacgttcaat accaactatg atattggtag tacttgcact tatttggttt
     7081 gaaaaggata gtaccccttt tatagttgga tttactatag tttttcctat attgtatgat
     7141 aacgttttgg gcgctatatt gaatatagac aagaacttat tagaaatggc taatgtgtat
     7201 aaaataaggt ttatagataa ggtgttaaaa atttatttac cagccataaa atttcaaatt
     7261 atatctatac tagtatcaac attctcttta agtttgaaag ttgctatagc aggagaggta
     7321 tatagtcaac ctacatatgg tattggaact atgatacaga ctgaaaaaat taactttaac
     7381 acatctggaa tatttgcatg gataattatt gttcttttaa tatctgctat tttacaaata
     7441 gctcaaaagt ttttagcaag gagagctttt ttatggaaaa gataaagttg agtattgaaa
     7501 atataaataa aagatatgat agtcgtatta tatttagaga ttttaatatt gatttttatg
     7561 taaatgaggt aaattgtatc cttggaaaat ctggttgtgg aaaaactaca ctcttgaaca
     7621 taataagtgg gattattaaa aatgacacca acaatctaaa tatcaaagaa aatttaaata
     7681 gagtaggaaa taagcttgaa gctagttaca tatttcaaga tgatagactt atagactggt
     7741 taacagtaga agaaaatata aagatagttg taaataaata ctataataaa actcaattaa
     7801 ataaaatttg tgatgaatac ttggaattgg ttggaattag tgattataaa aaattctatc
     7861 ctcagatgct tagtggaggt attagacaaa gagtaaatat tgctagagct tttatatatc
     7921 catcaaaaaa cattataatg gatgaacctt ttaaatctat tgatgcaaag aatacacagc
     7981 ttataatgga taatttcaga aatatattaa gaaaagaaaa aagaactgtg ttatttgtaa
     8041 ctcataatat tgaggaagca ttgtttttag cagataggat atttatttta ggagactctc
     8101 caattagaat aaaaaaaata ttaaaaaatt ctaaggaatt ggaaaaaaat gaagttttga
     8161 agttgatata gagttataat aatactgtcg ttttaaaata tatttggtta aaatctataa
     8221 gactatatta aaatagtaca gaatattcaa aactgtgtat aatgttcgta ttttataaaa
     8281 aggaagtgaa tgtatggtta agactaatgc tatgagaata ttagattcaa ataagattga
     8341 ttataaagta atgtcttatg aagtgaaaag tgaacatgtg gatggtgttg aggtggcaca
     8401 tgatatagga agagatgtaa atgaagtgta taagacatta gtaactcaag gagttagtaa
     8461 gaatatatat gtatatgtaa taccagtaca tgaaaactta gacttaaaaa aggctgcaaa
     8521 agtagctaaa gagaaaagtg tagagatgat tcatgtaaaa gatataaata agcttacagg
     8581 ttatattaga ggcggatgtt ctcctatagg tatgaaaaag ctttataaaa cttttgtaaa
     8641 tgaaagtgca aagaatttag atacaattat tgtgagtgca ggaaaaatag gatatcaaat
     8701 agaactatct ccatttgatt tacaaagact tataaaagta gaatttgtag atgtaataaa
     8761 aaaataagaa ttacatataa aatatagagc ttatattctt aataataggt gttgtaatat
     8821 taataaaatg aataataatt ttaaaataaa tattcagtta cttatgtgta attggtataa
     8881 aaatattgag aaattttaaa aacaatggta aagtgtaagt ataatcacga atatttttta
     8941 taaagtaatg gagggatgtt cgcttgagaa aaataaaatc tgaacaaatc gtcgagcagg
     9001 taaaaaaact ttgtatagaa gcgagcctat atttaggaga agatgtttta agttgtataa
     9061 aagaaaaagc gaaaagcgaa aagagtgaag ttggaaaaaa tatattgaat atattagttg
     9121 aaaatgctga aatagctaaa gaaaaaaata taccaatatg ccaagatact ggtatggctg
     9181 tattttttgt tgaaatagga caagaagttt taattgaagg agacacactt actgatgcta
     9241 taaatgaggg agtaagacaa gggtatgaag aaggctactt aagaaaatct gttgtaagtc
     9301 caattaacag agtaaataca aaggataata cacctgctgt tattcattat gatatggtaa
     9361 aaggtgataa aattaaaata gaatttgcag ctaagggatt tggaagcgaa aatatgagta
     9421 agatgaaaat gttaaagcca tcagatggat tggaaggaat taaaaaattc ataatagata
     9481 cagtttcaga agctggacct aacccatgcc ctcctatggt aattggagtt ggtataggtg
     9541 ggactgtgga caaatgtgct caaatagcta aaaaggcact ttttagagaa ctaggtgaat
     9601 ttaataagga cgaaaatata gcaaaacttg aaagtgaact attaacagct attaataaac
     9661 ttggtatagg acctcaaggc ttaggtggta ctacaacagc tttgggatta aacatagaga
     9721 cttttcctac acacatagca ggtttacctg ttgttgtaaa tataaactgc catgcatcaa
     9781 gacataagaa ggtagtaatt taggaggaaa ataaaatgat aaaaataact acacctgtaa
     9841 atgaaattga tatagcaaaa ttaaattgtg gagatacaat tagtttgagt ggtatacttt
     9901 atacagcaag agatgctgct cataaaagat tgatagattg tataaataaa ggtgaagaat
     9961 taccatttga cgtatatggt caaggaatat attatgtagg acctactcct acaaagccag
    10021 gagaagttat aggtgctgca ggtccaacga cgagttatag aatggatgat ttgactatac
    10081 ctcttttaga aagaggtctt agattgatga taggcaaggg aaaaagaagt gatgaagtta
    10141 tagaaggtat gcaaaaatac ggagcagttt atcttgctgc aataggagga gctggagcgt
    10201 atatttcaaa ttcaataaaa tcatgtgaaa ttattgcata tgaagattta ggagctgaag
    10261 ctataagaaa gattgtagta gaagacctta agcttacagt agcaatagat agctatggaa
    10321 ataatatata tgagcaaggt cgagcaattt atgaatgtaa ataatgttta gaaataaata
    10381 tgaatatcaa aattaaaaat agttgtgtat attttaagga ggaaaattga tgtctaagga
    10441 ttatgcaaaa ttggcattag aaatgcatga aatcaacaaa gggaaagtaa gtgtagaaag
    10501 caaggtagaa gttaaaacta aggatgattt atcaacagca tatacaccag gggttgctga
    10561 accttgttta aaaatacatg aaaatcaaga tgatgtttat agatacactt ctaaagggaa
    10621 tctagtagca gtagttagtg atggaagtgc tgtacttgga ttaggtaaca ttggtgctga
    10681 agcatctata cctgttatgg aaggtaaagc tatattattt aaacaatttg cagatgtaga
    10741 cgcttttcca atatgtttaa agactaatga tgtagatgag atagttaaat ctgtagagtt
    10801 gatggagcct gtatttggag gtataaattt ggaagacata tcttctccaa gatgttttga
    10861 gatagaagat agattaaaaa aatctttaag tatacctgtt tttcatgatg accaacatgg
    10921 gacagcaata atagttgcag cagccataat aaactcaata aagttaattg aaaataaaaa
    10981 aatagaagat ttagaaatag ttataaacgg agcaggagct gctggtatag ccatagcaaa
    11041 gatactactt aatatgaatg ttaaaaatat aatcttatgt gatagaagtg gagcattgga
    11101 agctagtata gaaaatctaa actatgttca aaaagaaatg ttaaaagtaa caaatataag
    11161 aaatgaaaaa ggacctttaa aagatattat aaaaggaaaa gatgtattta ttggcgtttc
    11221 aggtccagga gctgttacaa aggaaatggt tgaaagtatg tctgagaagc caataatatt
    11281 ggcaatggca aatcctactc cagagataat gccagaagaa gcaaaattag gtggagcatt
    11341 tataatggga acaggtcgtt cagacttccc taatcaagtc aataatgtac tggcttttcc
    11401 tggaatattt agaggtgcac ttgatgttaa ggctaaagag ataaatgaag aaatgaagat
    11461 agctgctgca tatgcaattg ctaatacaat aagtgatgaa gaaatatgcc cagagtatat
    11521 attaccagat gtatttaata agaatgtagt aaaaaatgtt gctaaggcag ttaaagaagc
    11581 agcgataaaa accaaagtaa atagaatata aatttttata tgttcatata tccttatctt
    11641 atcgggtata tgggcatttt tattataggt ttatactca
//
LOCUS       DAEMHW010000118        11664 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEMHW010000118 DAEMHW010000000
VERSION     DAEMHW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.118"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(93..1007)
                     /locus_tag="KPK67_002582"
     CDS             complement(93..1007)
                     /locus_tag="KPK67_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HBG2459530.1"
                     /translation="MNKKFRLYQVDSFTKEKFTGNPAGVITNADGLSESQMQKIAREL
                     NNSETAFIFNNLAKNHQENYDVHIRFFTPTNEVPICGHATIAAHYVRAVENQFNNKTV
                     FQKTGAGILPVDIIKKDNDYEIIMTQGKIEVSPPLQQTYQKRLLNAFGILKEDIRTDC
                     PIAISSTGHSKVMIGIKELSTLNSLSPNLYELSKISQEIKCNGHYIFTLHNKENLQVH
                     GRMFAPAIGISEDPITGNANGPLGAYLVHYKVIPIPNTEFIFEAVQGEAIKRKGKMLV
                     KVIILDNKPVEVKIIGKAVIAFSTNIEI"
     gene            1263..1619
                     /locus_tag="KPK67_002583"
     CDS             1263..1619
                     /locus_tag="KPK67_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2459531.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            complement(1734..1910)
                     /locus_tag="KPK67_002584"
     CDS             complement(1734..1910)
                     /locus_tag="KPK67_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459532.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     regulatory      complement(1963..2052)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      2681..2780
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2911..3201
                     /locus_tag="KPK67_002585"
     CDS             2911..3201
                     /locus_tag="KPK67_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBG2459533.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     gene            3176..3946
                     /locus_tag="KPK67_002586"
     CDS             3176..3946
                     /locus_tag="KPK67_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2459534.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            3968..4981
                     /locus_tag="KPK67_002587"
     CDS             3968..4981
                     /locus_tag="KPK67_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2459535.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            4950..5684
                     /locus_tag="KPK67_002588"
     CDS             4950..5684
                     /locus_tag="KPK67_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459536.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            5845..8688
                     /gene="mutS"
                     /locus_tag="KPK67_002589"
     CDS             5845..8688
                     /gene="mutS"
                     /locus_tag="KPK67_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBG2459537.1"
                     /translation="MQIDINKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KKYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            8699..10666
                     /gene="mutL"
                     /locus_tag="KPK67_002590"
     CDS             8699..10666
                     /gene="mutL"
                     /locus_tag="KPK67_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBG2459538.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            10704..11639
                     /gene="miaA"
                     /locus_tag="KPK67_002591"
     CDS             10704..11639
                     /gene="miaA"
                     /locus_tag="KPK67_002591"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBG2459539.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKGKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYKNIDELKNEIILYIKDSIK"
BASE COUNT         4750 a         1270 c         1879 g         3765 t
ORIGIN      
        1 ataataaaaa aattaatttg attgtatata aattagtttg ttattaaatt agtattaaaa
       61 agttcaaaaa tttacttaga aggaatctat aattagattt ctatgtttgt tgagaatgca
      121 attacagctt ttccaattat ttttacttca actggcttat tatctaaaat aattactttt
      181 actaacatct tgcctttgcg ttttattgct tctccttgaa ctgcttcaaa tataaactct
      241 gtgttgggta taggtattac tttataatgt acaaggtacg cacctaaggg tccattagca
      301 tttcctgtaa tagggtcttc tgaaatacca attgcaggag caaacattct tccatgaact
      361 tgtagatttt ctttattatg taatgtaaaa atataatgtc cattacattt tatttcttga
      421 cttattttcg ataattcata taaatttgga gataaactat ttaatgttga taattccttt
      481 attccaatca taacttttga atgtcctgta gatgatatgg caataggaca atctgtacga
      541 atatcttcct ttaaaatacc aaatgcatta agtaatcttt tttgataggt ttgttgtaat
      601 ggtggagata cttcaatttt tccttgtgtc ataattattt cataatcatt atcttttttt
      661 ataatatcaa ctggtaatat tccagcacca gttttttgaa atactgtttt gttattgaat
      721 tggttttcta ctgctctaac ataatgggca gcaatagtag catgaccaca tataggaacc
      781 tcattagttg gtgtaaaaaa tctgatatgt acatcataat tttcttgatg atttttagcc
      841 aagttattga aaataaaagc agtttctgaa ttatttaatt ctcttgctat tttttgcatt
      901 tgtgattcag ataagccatc agcatttgta attacaccag ctggattacc tgtaaatttt
      961 tcctttgtaa atgagtcaac ttgataaaga cgaaattttt tattcatatt atataatcct
     1021 ttctaaacat tagattagtt tatctacata gaaaacttga gataaattat tatttattat
     1081 tatctgttta aataaaatta ataaaataac atatgtctat attaaaattt aaacatatac
     1141 atctaaaaaa gtagtattat gtaaggctct atgctacaat caagattata ttacgaatta
     1201 gtacgctaaa caagaatgca ctttttagtt agatacttac tattttgaaa gagaggattg
     1261 agatggatat agataataat tttgaagaat ttccaaatat tgaagaaaga caaaaagatt
     1321 ttaattgttc aattggtttt accatgactg tgataggaag taaatggagg gcaattattt
     1381 tatggcatat tttaaaacaa gagccaatac gttacagtca attaaaaaaa gaagttgttc
     1441 atataagtca taagatactg tcacaagaat taaagtatct tgaaagagat ggacttatta
     1501 agaggatttc ttatccgact attcctccaa aagttgaata tttgtctact aaacgtggta
     1561 aatcattaga atctatatta ttagaattat gtaattgggg taaacagtat atgagataaa
     1621 ctcaactgtt tactttatga aaaatatata atgaagctta ttttaattag ttctttaatg
     1681 aattataaaa cgtagataaa actaaaaagg gttttctaga aaataaaatt tctttaatta
     1741 tatctgctta ccaataccat cacttgatgc aaacaagatt ctaccacatt ctctccatat
     1801 tctagtataa aatcatttaa ttgtgtatct ataaaattgc agtaatcgcg tgaactgcat
     1861 atatatgcag ttagtatatg tgtacatcta tccattttat ccataaacat atatcatata
     1921 tccccctaaa aaacattcat aaaaacaaaa agaaaatatc tctttggcaa ctggctgaca
     1981 taactcattt cataagtctt agtccctata gctttgcgtc actaaatttc tctagttttg
     2041 cctatttagt tttattctac tattaccata atataggaaa acgtttcttt tttcaaggtg
     2101 catattaaaa aatttaaaaa ttaaaaaaat attttatttc tcaaaaataa attggataaa
     2161 ataattatat tatagagttt gattcttatc taaaatgttg gttgctgagg attttgatga
     2221 taatgacgag taatttttat attatcacaa aagatacttc ctaaaaggct tgtatcttaa
     2281 aaatccagat agaggtttat ttttagatta ctgaatatta ttactgaatt tattgaagga
     2341 ttttttgtag ataaagataa gtagagtcta aaagctattt ctaatctgta atctgtaacg
     2401 attgcaaatt agattagtga aaacagaaat ttagaacaat atttgtatgg aattgatata
     2461 catccaaata tgcttgaaat agcaaaataa aaactagaaa attcagttaa tctagttttg
     2521 gaagatgttg ataaattacc attttaagat aatttatttg gtgtaataat gtacaattta
     2581 tattagatat ttttgaacgg ataaaatatc taatataaat aatataaata aaaaatttgt
     2641 taaaatatga taagtgtagt ataatcaagt agaaaagata taatatattt cggggagcta
     2701 atgtgctgag aggagatttt atctcgaccc tttgaacctg tttggataat gccagtgtag
     2761 ggagaccagt taagataaaa ataagtctta tcctattttg gataagactt ttcttttatt
     2821 taaatataat atctttttag taataaaatt ttttaataaa aaacaaaata tacctagtag
     2881 gtattaatta tttttaaagg agtgatttaa atggtaatcg cagacatagc agtaatgcca
     2941 ctaagacctt acaaaggtga agaagagatg tataaagtag tagatgcttg tattgctgaa
     3001 attgaaaaaa gtggtcttaa atgtgaaata ggagcaatga gcactactgt tgaaggtgat
     3061 tttgatgaag tttttgaatt attaaaaaag gtacataaaa tcccttttaa tcttggatgt
     3121 gaaagagtca taacagtagc tagagtagat gaaaaagctg gaggtctgac tatagatgaa
     3181 aaacttagaa atcatagata aagtcaaaga tagtatatat ccaatagcaa catttttgtt
     3241 tatacttatt ctttggcaat ctatggtagg agttttagaa gttcctcagt atatactacc
     3301 tacaccagta gatataatta atgtgttttt taaagattat caaaatttat ccatgcatgc
     3361 tgtggttact ataggagaag caatacttgg ttttatagtc gcaattataa tatcattggt
     3421 aattggaata ttgatggatt ttgtatcaat aataaaaaaa tgtctttatc ctattatgtt
     3481 ggttacacag atgataccta caataattat agcaccactt tttatgatat ggtttggatt
     3541 tggaacaatg ccaaaagtat tgatggtaat gcttacttgt ttttttccta tattaataag
     3601 ttttgtagat ggcattgaga atattgataa agattattta aatttattta agactatgga
     3661 ctcaaataaa gttaatacat tcattcacct taaatttcct atggcaatgg ataaattttt
     3721 ttctggactt aaaatatctg caacgtatgc tgtaatggca gcaactgttg cagaatggct
     3781 aggtggcacg aaaggacttg gtgtttatat gcttaggtca aaaagtgcat atgcacttga
     3841 taaagtattt gcatctacta tattagtagt tatatttagt ttaatgtttg taggaattgt
     3901 acaggctgta aaaaaagttg ttataagaca tagattaata gattaaaggt atttgaggga
     3961 ggcaattatg aattttaaaa agttaggaat attaggttta gtaagtgttt ttacattggc
     4021 aacaataact ggatgttctt caaataacaa taaagacaaa aacaatgata aagatacttc
     4081 ttctaaaaaa gtaactatgg tgcttgattg gacaccaaat acaaatcata ctggattatt
     4141 tgtagcactt gataagggtt actataaaga agaaggtttg gatgttgaaa tagtacaacc
     4201 acctgaaagt ggagcagaaa ctttagtagc aacaggaaaa gctgactttg gtataagcta
     4261 tcaagaacaa gttacttatg caaagactag tgaagatcca cttccaataa aagctgtagc
     4321 aactgttata caacataata cttctgggtt tgcatcacca aaagaaaaga atataacaac
     4381 agctaaagat tttgaaggaa aaacttatgg tggttggggc tctccatcag aagaagctgt
     4441 ttttaaagca gttatgaaga aaaatggagc tgattttaat aagttaaaga tagttaatac
     4501 tggtcaagat gatttctttg cagctatgaa aactgtagat tttgcatgga tatttgaagg
     4561 ttgggatgca gttaaagctg atttaattgg atatgactta aactttatac cagtaaaaga
     4621 tttagatgag agattggatt attatacacc acttataatt tctaatgaaa ctgtgcttaa
     4681 agataatcca gagcttgcta aaaaattctt aaaagctaca acaaaaggtt atgaatatgc
     4741 aataaaaaat ccagaagaat cagcaaaaat tttagtaaaa catgcacctg aagtagatga
     4801 aaaactagct ttaaagagtc aagaatattt agcaagcaag tacaaagatg atgcaccacg
     4861 atggggagaa atgaaagata gtgtatggaa caactataca tcattcttaa aagaatataa
     4921 attaatagat aaggatatga aggcaagtga tgcttacaca aatgaatttt taccacaata
     4981 aaaaagtaaa agtatctata gaaaatgtat caaaaactta taaagatatt gaagttttaa
     5041 aagatataag tattgatgtt tatgaaggtg agtttgtttc tatattaggg ccaagtggat
     5101 gtggaaaaag tactattttt aatattataa ctaaacttac agattatgat agtggcaaag
     5161 tgaatataaa tggtaagtat agttatatgt atcaaaaaga tttactcttg ccatataaaa
     5221 ctataataga taatgtgtca ctaccactaa ttttaaaaaa agaaaaaaag agtaaagcta
     5281 ggtcaatggt aaaaccttat tttgaggtgt ttggattaag cggatatgaa gataaatatc
     5341 cttcagaact ttctggagga atgagacaga gagctaactt cttgagaact tttataaatt
     5401 ctaatgacat aatgctttta gatgagcctt ttggggcact tgattcatta actaaaacat
     5461 ccatgcaaaa atggctttta gatgttaaga aaaaagtaaa ttcaacaata cttcttataa
     5521 ctcatgatat tgacgaagct ataatgctat caaacagaat ttatgttatt tcaaaaaaac
     5581 catcaatagt aaagaaagaa tttgttatag atagtagaaa gttaaatgaa gataaccttg
     5641 aaaatatcat taaacttaaa aaagaaataa tatctttatt gtaaaaaaaa atatttttat
     5701 ttaataagca ttaaacttag caatccaaaa tgtgagtata cattttggat tgtttttagt
     5761 tatatgtcaa aattttatat attgtgttaa taataatcat ctactatgat ataatattta
     5821 attagaaaag aaggaggttt gataatgcaa atagatataa ataaattaac tcctatgatg
     5881 aaacagtatc ttgaagtaaa aaatagatat aaagactgta tactgttttt tagacttgga
     5941 gatttttatg aaatgttttt tgaagatgca ttagtagctt caaaagctct tgaaatagca
     6001 ttgacaggaa aagcttgtgg tttagaagaa cgtgctccta tgtgtggtgt tcctttccac
     6061 tcagccaatt catatatatc aaaattagtt gaaaatggat ataaagtagc aataggtgaa
     6121 caaatggaag acccatctac agcaaaagga attgttagaa gagaggttat aagagtcatc
     6181 actcctggta cagttttaga tggaaattta cttgaaaata aaaagaataa ttatttatta
     6241 tctttgtata aggatggtac taatataggt ctaacttatg ttgatataag tacaggagaa
     6301 actaatgcaa catgtttaaa tgaagataag gtaattgaag aaattgcaaa aatccatcca
     6361 actgaaataa ttataaatga tttagacttt atagaaaagc ttagagatat agctactgta
     6421 agtaatatat acataaatga aagttttagt gataactatt tggatataaa tatattaaaa
     6481 gaatattttc cagatgttta tctacaaaaa ctgaagtttg atgataaagg tctaataaag
     6541 tcaagcttat caattcttct gaattatata tacaatactc aaaaacaaat aacatctaac
     6601 attaataata taaatatata caattcatca gaatatatgg ttttagatat gtttactaga
     6661 actaatttag aacttactca aactataaga gggaataaga aaaaaggttc attattacac
     6721 gtacttgata aaacttctac agcaatggga ggtagacttt taagaaaata tgtagaagaa
     6781 cctttgataa ataaatctaa aattgaaaat agattagatg taatagaaga aataaaagat
     6841 gactttatat taagagaaga cctaaatgat atacttaaaa atatatacga tatagaaaga
     6901 atatgtggaa agatagcatt tgagagagta acaccaaaag agttaattca cttaaaaaat
     6961 tcgatagaaa agttacctaa tttaaaagat actataaatt tatcaaatgc aaaaatccta
     7021 aaaaagtatg tttcagaaat ggataaatta gatgatatat acaatttaat agatgaagct
     7081 attctagaag aacctacgat aaccataaaa gatggaaaca taattaaatc agattttagt
     7141 gatgagctta aggaactaag agagatttct aagaatggag cctttcttgt aaaggaaata
     7201 gaaaacaggg aaagagaaaa aacaggagtt aaatcattaa aaataggatt taataaagtt
     7261 tttggatatt atatagaaat aacaaaagcc aactttaaac aggcaaagct agatgagaca
     7321 tatataagaa agcaaacact tagtaatgca gaaagatata taactccaga attaaaggaa
     7381 atagaagaaa agatactaca tgcagaggaa aagattaaat ctttagaata cgagatattt
     7441 gtagagataa gagatacaat atataaaaac atagatagaa tacaaaaagt ggctaagact
     7501 atagctaata tagatgtttt tgtgtccctt gcaacagttg cacatataaa taactatgta
     7561 aaaccagcaa taaatgaaaa taataaactt gatataagaa atggtagaca tcctgttgtc
     7621 gaaaatatag taggagagga aaatttcgtt ccaaatgata cttatttaaa tcgtggagaa
     7681 aacattatta acataataac tggtcctaat atgtcaggta aatcaacata tatgagacaa
     7741 actgctataa tagctcttat ggcacatata ggctcatttg taccagcaga atctgctgat
     7801 attccaatat tagatagaat atttacaaga gttggagcta gtgacgattt atctcaaggt
     7861 caatctactt ttatggtaga gatgaacgaa gtatccctta tattaaaaaa tgcaacagag
     7921 agaagtttag ttatattaga tgaaataggt cgtggaacta gtacctatga tggtataagt
     7981 ttagcatggt caatagtgga atatattcaa aaaaatatta gatgtaagac attatttgca
     8041 acacattatc atgaacttac tgatttagaa gaagagttta aagaggttaa aaattattca
     8101 atagcagtca aagaagatgg ggaaggaata atatttttaa gaaaaataat tcctcaggga
     8161 gcagacaaga gttatggaat atatgttgct aaattagcta aattaccaga cgaagtcata
     8221 gaaagagcaa aatatatact taaagacttg gaaaaaaatc atgtttacaa cagtgttgca
     8281 ataaatggag ataaagaaaa caataatata attaatagta atttggacga agaattagtt
     8341 aaggtaaatc aaagtaattt taagaataaa tatgaatctc taaaaataga acatgaactt
     8401 atagtaaaag actacaaaca tataaaaaag gattatgata aattaaattc taaatttaaa
     8461 gcattaaatg atgaagttgc tttaatgaag caaaatgatg ataaagagaa aattaatcaa
     8521 gaggattctg taaaagaagt agccttaact caaatctctt ttgatagtgt aaatagagat
     8581 atactatcag aagaaatttt aaatctggat attttaaata tgactccatt agatgctata
     8641 aactctcttt ataatcttca aagaaaagca aaagaaataa aatcttagag gtgtaaaaat
     8701 gaaaaatata attaacatcc tagatgattt gactataaat aagatagcag caggtgaagt
     8761 tgtagaaaga ccttcttctg tagtaaaaga acttattgaa aactccattg atgcaggtgc
     8821 aaataaaatt tccatagata taattgatgg aggaaaaagt ctaattaaga ttacagataa
     8881 tggaatagga ataccttcaa gtgaagttga aaaatcattt ttgagacatg ctactagtaa
     8941 aattaaaaaa attgatgatt tgtatgattt atactctctt ggttttagag gagaagcatt
     9001 ggcatctatt tcagctgtat caaaattaga aatgacgact aaaactaaag atgaaataat
     9061 aggaactaaa atatatgttg aaggtggaaa aataatatcg aaagaaccaa taggttctac
     9121 taatggaact accatcatta taaaagatat tttctttaat acaccagcaa gacagaaatt
     9181 tttaaaatct actcatgcag aaactataaa cataagtgat ttgataaata aacttgcaat
     9241 aggtaatcca aacattcaat ttaaatatac aaataacaat aaacagatgt taaatactcc
     9301 aggtgatgga aaacttgtaa acactattag aagtatatat ggaaaagaaa taacggaaaa
     9361 tataatagat gtagagttta agtgcaatca ctttaaaatg aatggatata taggtaataa
     9421 caatatatac cgttcaaata aaaacttaca acatatctat ataaataaaa gatttgtaaa
     9481 aagtaaaata atcatagatg caataacaga aagttataag tctataattc caataggtaa
     9541 acatgcagtg tgcttcttaa atatagaagt agacccatca tgtatagatg taaatataca
     9601 ccccaataaa cttgaaatta aatttgaaaa agaacaagaa gtgtatatag aacttagaga
     9661 ttttttaaag gttaaattaa tacactcaaa tcttattgga aaatatgcta cttatagtga
     9721 taaaaaaaca caacctagaa tcgctattaa cagtagagaa aaaagtacag attataaatt
     9781 aagaaataat gatttattag aatctactcc taaaaatagt aatattacta aaggtaagga
     9841 tgaagtcatt gaagttgtta ctttatcaag tgaaaaacca ataaatgaat ttcaatcagt
     9901 aagtgaagtc ctaaatgcaa gtgtagaaga tgatgtaaaa aacattaatt atctaagtga
     9961 agatagtgca aatgataata tacaagaaga gtttcaagtt gatggaatta aaaatgaagg
    10021 aaattattac ttaggagata gtattaagga cagtgaagaa gaatacttgt gtagttcaaa
    10081 acgcaagttt tctttatacg gatattctgt aattggagtt gtatttaata cttatataat
    10141 tttgagcaaa gatgattcta tgtatttgtt ggaccaacat gctgcacatg aaagaatact
    10201 atatgaaaga tatatggaga agttctatag acaagatata aatatgcaaa tacttttaga
    10261 cccagtggtt attgaagtat caaatgtaga tatgcttcaa attgaaaata acttggagtt
    10321 atttatgaaa tttggttttg agctagaaat ttttggcaat aatcatataa tggtaagatg
    10381 tgttcctact atatttggag tacctgaaac tgagaaattt atacttcaaa taattgataa
    10441 tatagaagaa attacaagta attatgattt aaaaggagag agatttgcat ctatggcatg
    10501 taggtctgct ataaaagcaa atgataaaat atacgatatt gaaataaaaa gtttattgga
    10561 acagctagaa aagtgtgaga atccatttac atgtcctcat ggaagaccta taatggtcga
    10621 aatatctaag acagaaatag aaaaaatgtt taaaagaatt atgtaaaata tttggatatt
    10681 taaaatatat ggaaaggaat gttatgaaaa aaataccact tataatactt acaggtccaa
    10741 ctgctgttgg aaaaacagat ttgtcaataa aattagcaaa agatatgaat gcagagataa
    10801 tttcagctga ttctatgcaa atatatgaat atatggatat tggaagtgca aaagttacag
    10861 aggaagagat gcaaggagtg aagcattacc ttatagatga agttaagcct gactatccat
    10921 tttcagtttc agagtttcaa caaagagcaa aaaaatatat acatgaaata aatgaaaagg
    10981 gaaaatgtgt tttagtaact ggaggcacag gactttattt aaactcttta atatataata
    11041 tggattttgc acaatctgat gccaataatg aattaagaga agaacttcaa aaacaacttg
    11101 ctgaaaaggg aatagattac atgcataata agttaaaaga acttgatgaa gaatctgcta
    11161 atagaatcca taaaaacaat actaaaaggg tcataagagc tcttgaagtt tgtctaagtg
    11221 gtaagaaaat gaatgatttt tcaagtgatt taaaatttaa tgaagaatat caaccaataa
    11281 taatagttct aaatagagat agagaacatt tgtatcaaag aataaacatg agagttgata
    11341 ttatgcttaa aaatggattg gttgaagagg taaaaaaact tttaagtatg ggatttaaaa
    11401 aagatatgat atcaatgcaa ggtataggtt ataaagagat attaaagtat ctggatggag
    11461 aatatacata cgaagaagct attgaaataa ttaaaagaga ttctagaagg tatgcaaaac
    11521 gtcagataac atggtttaaa agatataaaa cagcaaaatg gtttgattta gaccaatata
    11581 aaaatataga cgaattaaaa aatgaaataa tcttatatat taaagattct ataaaataat
    11641 atataaattt ttaagtatac aact
//
LOCUS       DAEMHW010000119        11627 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEMHW010000119 DAEMHW010000000
VERSION     DAEMHW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.119"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(192..2282)
                     /locus_tag="KPK67_002592"
     CDS             complement(192..2282)
                     /locus_tag="KPK67_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBG2459540.1"
                     /translation="MILVIAEKPSVAQTIAAVLGAKEKKDGFLTGSGYIVSWCVGHLV
                     GLAEAAAYGEQYKKWSYDSLPILPQEWKYTVSADKEKQFKTLKELMHRADVSKVVNAC
                     DAGREGELIFRFVYEMAGCKKPMRRLWISSMEDSAIKEGFSRLKNGEEYDALFASALC
                     RAKADWLIGINATRLFSVLYNHTLNVGRVQTPTLKMLVDRDAAITTFKKEKYYHVRLA
                     LSGAEAASERISDKAEADALKTTCEASKAVCTSLVKEKKTAAPPKLFDLTSLQREANR
                     LFGYTAKQTLDLAQALYEKRLLTYPRTDSSYLTDDMGDTAAGIIKLLCGKFPFMAGKD
                     FTPELGKVLNSKKVSDHHAIIPTMELAKTDLAALPESERNILTLAGTRLLMATAAPHT
                     FEAVTAVFECAGQSFTARGKTVLSDGWKEIDRRYRAALKSKPETDDADSDIEKTLPLF
                     TEGQTFDNPAATVTEHDTTPPKPHNEASLLSSMERAGSEDTDPDAERKGLGTPATRAA
                     VIEKLVKGGFVERKGKQLLPTKDGINLVCVLPDTLTSPQLTAEWENNLTQIAKGKADP
                     AAFMEGIEDMARELVKTYPFLSDDKAQMFKPEREALGSCPRCGSPVYEGKKSYYCSNK
                     DCIFTMWKNDRFFEERKVTFTPKIAAALLKSGKVNVKKLYSPKTGKTYDGTIELADTG
                     GKYVNYRIELSKKK"
     gene            complement(2407..2769)
                     /locus_tag="KPK67_002593"
     CDS             complement(2407..2769)
                     /locus_tag="KPK67_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2459541.1"
                     /translation="MKLKNILIVVKDIEKSKQFYNDLFGLDLLLDNDGNMILTEGLVL
                     QDEKIWKKFLGKDIIPKSNSCELYFEERDIEAFVEKLERSYPSIQYANKLMTHSWGQK
                     VVRFYDLDGNLIEVGTPM"
     gene            complement(3046..3774)
                     /locus_tag="KPK67_002594"
     CDS             complement(3046..3774)
                     /locus_tag="KPK67_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBG2459542.1"
                     /translation="MKNKLKKTMTALCAALILMGGFSVPAYAQGAATEPPAGDATNDS
                     GVIVEEKEETPPLTPKGNATLVDDFGGNKQLITVTTKGGNYFYILIDRAAEGEQTVHF
                     LNQVDEADLMALTENGEATKKPEICNCTEKCEAGKVNTSCPVCVTSMTGCTGKEPTPT
                     PTEQPEEPKEKGGNPGVVLALVLFALLGGGAAFYYFKFMKPKQNVKGDTDLEDFEFED
                     YDEDEPEADTGETSEDEETEEETL"
     gene            complement(3764..4021)
                     /locus_tag="KPK67_002595"
     CDS             complement(3764..4021)
                     /locus_tag="KPK67_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBG2459543.1"
                     /translation="MAMNKIDRINKEIAKTREKITEYQNKLKGLEAQKTEAENLEIVQ
                     LVRAMRLTPQELNAMLSGGGIPGMTAAPAEENEQEENADEE"
     gene            complement(4053..6089)
                     /locus_tag="KPK67_002596"
                     /pseudo
     CDS             complement(4053..6089)
                     /locus_tag="KPK67_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585096.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
     gene            complement(6139..6411)
                     /locus_tag="KPK67_002597"
                     /pseudo
     CDS             complement(6139..6411)
                     /locus_tag="KPK67_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905270.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
     gene            complement(6529..8319)
                     /locus_tag="KPK67_002598"
     CDS             complement(6529..8319)
                     /locus_tag="KPK67_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBG2459544.1"
                     /translation="MKPTSEILERIEKCSSEHKDGVFTRLYRYLLREDIYYAAYQKLY
                     ANKGATTQGIDDDTADGFSDFYVKELIQSLKDGTYKANPVRREYIPKKNGKLRPLGIP
                     SFRDKLLQEVVRMILEAIYEPVFDSHSHGFRPSKSCHTALRQISSDFTGVVWFIEGDI
                     QGCFDNINHEKLIEILSKKIKDSRFLNIIRQFLKAGYIENWKYNATYSGSPQGGICSP
                     ILANIYLNELDKKFREIAERFDKPRSAYQTPEYHAASKELKRLSYWIDHTADEATRQE
                     LIDQHKAQKKAMRNLPCKPADNKKFTFVRYADDWLAGVCGTKTECEELKAEIAEFLSR
                     ELKLTLSEEKTLITHSSEKVRFIGYDICVRRNQEVKGHRMKNGTWRKSRTLHMKVALS
                     IPHTEKIEKFMFAKKVIRQKENGEFQTIHRAGLLNLADYEIVEQYNAEARGLCNYYNL
                     ACDYHTLDYLCYLMEYSCLKTIANKHKTSIRKIIRQYKDGKTWSVPYETKAGTRRVRP
                     VKIADCKRGEASDIIYQRKKFSWKTTIRQRLNARVCELCGCKEADLYEVHVIRNLNEL
                     GNSDWETVMKKKRRKTLVVCSKCHERIHRH"
     gene            complement(8813..11011)
                     /locus_tag="KPK67_002599"
     CDS             complement(8813..11011)
                     /locus_tag="KPK67_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBG2459545.1"
                     /translation="MSKGQTKKTREAAGNRRKLTRAEKKQIAEAIRKAKSDGKACTAQ
                     QTIPYLAMYPDGICKVTEKRYSKCVVFEDINYQLAQADDKTAIFENWCDFLNYFDASV
                     SVQLSFINQGTQREQAEKAISIPAQEDAFNSIRTEYSDMLKNQLSKGNNGLVKHKYIT
                     FTVEADNKAAAKSRLSRIETDVLNNFKVLGVTARPLSGYERLKVLHGVFHPEGEPFSF
                     SFDWLTPTGLTTKDFIAPSSFRFGEGRYFRMGKKIGAASFLEILAPELNDRMLADILD
                     LETGVIVNLHIRSIDQSEAIKTIKRKITDLDKMKIEEQKKAVRSGYDMDIIPSDLATF
                     GSEAKNLLQDLQSRNERMFLLTFLVVNMADTKRKLENDIFAAAGIAQKYNCRLTRLDY
                     QQEAGLLSSVPIGENLIPIQRGLTTSSTAIFIPFITQELFQTGQALYYGLNALSNNMI
                     LCDRKQLKNPNGLILGTPGSGKSFAAKREMTNAFLITDDDIIICDPEAEYFSLVQRLN
                     GQVIRLSPTGRGIDGKPQYVNPMDINLNYSEDDNPLALKSDFILSLCELVIGGKEGLQ
                     PVDKTVIDRAVRNVYRPFLADPDPAKMPILGDLYDELLGQPEPEAARIAAALELYVSG
                     SLNVFNHRTNVELSNRLVCFDIKQLGKQLKKLGMLIVQDQTWNRVTINRAEKKSTRYY
                     MDEFHLLLKEEQTAAYSVEIWKRFRKWGGIPTAITQNVKVRPDRALCKAV"
     gene            complement(10959..11363)
                     /locus_tag="KPK67_002600"
     CDS             complement(10959..11363)
                     /locus_tag="KPK67_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBG2459546.1"
                     /translation="MAYVPVPKDLSKVKTKVAFNLTKRQIVCFAAALLIGLPLFFLLK
                     GSAGTNLAAMAMIVVMLPCFLLAMYEKHGQPLEVVVRNVIQTKFTRPKERPYKTENLY
                     AVLEKQRNLEKEVSAIVKRTNKKDAGSRRKQA"
     gene            complement(11385..11624)
                     /locus_tag="KPK67_002601"
     CDS             complement(11385..11624)
                     /locus_tag="KPK67_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459547.1"
                     /translation="MTNREWGQIGNNYLKGLFALGFQGFLIMICVGIYAVLVNDMIIA
                     DNLHSAVFSLAAYTVILCFSLFKSGTLAKSIFNAH"
BASE COUNT         2519 a         2904 c         2749 g         3455 t
ORIGIN      
        1 aaatggcagc gcggacgctg cattttcctg tttcatcatt cagtaccccg cgcccgcatt
       61 tttgccaaag atttttcaag atttcagagg gtttgggaaa cttcccaaca agcaaaaatc
      121 cccgccgttc tcggtagaga aagcagggat tttacggaaa agggtactct tttcctatgc
      181 ttgctattca gttatttctt cttcgacagt tcaatgcggt aattgacata tttcccgccc
      241 gtatcagcta attcgatagt tccgtcgtag gttttgcctg tctttgggga atagagcttt
      301 ttcacattta ctttgccgga ttttaagagt gcagcggcaa tcttcggggt aaaggttact
      361 tttcgttctt caaagaagcg gtcatttttc cacatggtaa agatacagtc cttgttactg
      421 caatagtagc ttttctttcc ctcatagaca ggagaaccgc agcgggggca gcttcccaat
      481 gcttcccgtt ccggcttgaa catctgcgct ttatcatcag aaagaaatgg ataggtcttt
      541 accagttccc gcgccatatc ctcgataccc tccataaatg cggcggggtc tgccttgcct
      601 ttggcaatct gcgtcagatt gttttcccat tctgcggtaa gctgcgggct tgtcaaagtg
      661 tccggcagga cgcacacaag gttgataccg tcttttgtgg gaagtagctg cttgcctttt
      721 cgctccacaa agccgccctt taccagtttt tcaatgacgg cggcgcgggt ggcgggagtt
      781 ccaagccctt ttcgttctgc gtccgggtcg gtgtcctcac ttccggcgcg ctccatagag
      841 gaaaggagcg acgcttcgtt gtgaggtttt ggcggtgtgg tgtcatgctc cgttaccgtt
      901 gccgccggat tgtcaaaggt ctgtccctcg gtaaacagcg gcagggtctt ttctatatcg
      961 ctgtctgcgt cgtccgtttc gggcttgctc tttaaggctg ccctgtatct tcggtcaatc
     1021 tctttccacc cgtcggacag taccgttttt ccccttgcgg taaaggactg tccagcacat
     1081 tcaaaaactg ccgtaaccgc ttcaaaggta tgcggtgcgg cggtcgccat aagtagacgt
     1141 gtcccggcaa gggtaaggat attcctttcg ctttccggca gcgcggcaag gtcggtcttt
     1201 gcaagctcca tagttgggat aatggcatgg tggtctgata cctttttact gttcagcacc
     1261 tttccaagct ccggcgtgaa gtcttttccc gccataaaag gaaatttccc acaaagcagc
     1321 ttgataatgc ccgccgctgt gtcgcccatg tcgtcagtaa gatagctgct gtctgttctc
     1381 ggataagtaa gaagtctttt ttcatacagg gcttgtgcaa ggtcaagggt ctgttttgct
     1441 gtatatccga acaggcggtt tgcttccctc tgtaaagaag taaggtcaaa gagcttcggc
     1501 ggggctgcgg tctttttctc tttcacaagg gaagtacaga ctgcttttga cgcttcacaa
     1561 gtcgttttta atgcgtcggc ttctgcctta tctgaaattc gttcacttgc agcttccgcg
     1621 ccggataagg caaggcgcac atggtagtat ttttctttct taaatgtggt aatcgccgcg
     1681 tcccggtcaa caagcatttt gagggtcggg gtctgtacgc gccccacgtt caaggtatga
     1741 ttgtaaagga cagagaaaag acgggtggcg ttgataccga ttagccagtc tgcctttgcc
     1801 ctgcataatg cggaagcaaa gagcgcgtcg tattcctcgc cgttcttcag acgggaaaat
     1861 ccctctttga ttgcgctgtc ctccatagaa gaaatccaaa ggcggcgcat aggtttcttg
     1921 cagcccgcca tttcatagac aaaacggaaa atgagttctc cctcgcgccc tgcgtcgcag
     1981 gcattgacga ctttggaaac gtctgcccgg tgcataagtt cttttagggt tttgaattgc
     2041 ttttccttgt cagccgaaac ggtgtacttc cattcctgcg gcaaaatcgg taagctgtca
     2101 tagctccact ttttatattg ttccccgtag gcggcagctt ccgcaagccc taccaaatgt
     2161 cccacgcacc aagaaacgat atagccgctt cctgtgagaa atccgtcttt cttttccttt
     2221 gccccaagta cggcagcgat tgtctgcgcc acactgggct tttctgcaat aactaaaatc
     2281 aaaaataaat cctcctttcg ggtaaaagaa aagagcaacc gatttttcga ttgctcctcc
     2341 aagtgattgt ataaaattgt gcgatatgaa atagtaaaat gatttgacaa actgaaattt
     2401 atggtgttac atcggtgttc ctacttcaat caaattaccg tccaaatcat aaaaacgaac
     2461 tactttttgt ccccaactat gtgtcataag tttattagca tactgaattg aaggataaga
     2521 ccgttccaac ttttcaacaa atgcttctat atctcgttct tcaaaatata attcacaaga
     2581 attactttta gggataatat cctttcctaa aaactttttc caaatttttt cgtcttgaag
     2641 cacaagtcct tccgttaaaa tcatgttacc gtcattgtca agtagcaaat caagaccgaa
     2701 caggtcattg taaaattgtt ttgatttctc aatatcttta acaacaatta aaatattctt
     2761 taatttcatt gtctaacacc tctaaataaa ttccgatttg tttaactctg tcatttccta
     2821 tggttggaac aattatatca tattccatat cgcaagtaca tcttttttat cgttccgtct
     2881 gtcgtttggt ttccagtttt ctgatacagt accccacaca aggggactgc cccttgtaag
     2941 ggcaggaagc gcaagccggg ggataaggaa caggcggcgc attatcacgc cgcctgtccg
     3001 gtttctgtat catcatcttt tcaaagggat tgtcggtaaa cagggtcata gggtttcttc
     3061 ctccgtttct tcatcttcgg aagtttcccc ggtatctgct tccggctcgt cctcgtcgta
     3121 gtcctcaaat tcaaaatctt caaggtcggt atcgcccttt acattctgct tcggcttcat
     3181 aaacttaaag tagtagaacg ctgcgccgcc accaagcagg gcaaagagga ctaaggcaag
     3241 caccacaccg ggattgccgc ctttttcttt cggttcttcc ggctgctccg ttggtgttgg
     3301 ggtgggttct ttgcctgtgc agcctgtcat actggtaaca cataccgggc aggacgtgtt
     3361 tacctttccg gcttcgcatt tctccgtaca gttgcagatt tccggctttt tcgtggcttc
     3421 cccgttttcg gtcagtgcca taaggtcggc ttcgtccact tgatttagga aatgtacggt
     3481 ctgttcgccc tcggcggcgc ggtcaatgag gatatagaag tagttgccgc ctttggtggt
     3541 aacagtgata agctgcttat tgccaccgaa atcgtcaacc agtgttgcgt ttccctttgg
     3601 ggtaaggggc ggcgtttcct ctttttcctc cacaatcacg ccgctgtcgt tggtcgcgtc
     3661 cccggcgggc ggctctgtgg ctgcgccctg tgcataggca gggacagaaa aaccgcccat
     3721 aaggataagg gcggcacaaa gggcagtcat ggtctttttc agtttattct tcatctgcat
     3781 tttcctcctg ttcgttttct tctgcgggtg cggcagtcat gccgggaata ccgccgccgg
     3841 acagcatagc gttcagttcc tgcggggtca gacgcatagc tcgtacaagc tgcacgattt
     3901 caaggttttc cgcttcggtt ttctgcgctt ccagtccttt taacttgttc tgatactctg
     3961 tgattttctc gcgggtcttt gcgatttcct tattgatacg gtcgattttg ttcattgcca
     4021 taaataaatt ctcctttctt ttcggggctg ctttaccagt tcatcagccc gtagcctttg
     4081 atacattgat agttaaggtc atagctcttt atcttgcagg cgtcgccgga attgccctca
     4141 acggtataga cgcggctttc gtccctgcca agcacaagac ctacatggtc ggcaacgccg
     4201 tctaaatccc aatcgaaaaa gatagcgtcg cccggtgcga tattctcata gccgcgtgcg
     4261 ccccattgtc cccttgacta aaaccaaggg acaccctgcc attcgcagcc cgcaaatcgc
     4321 ggttcgcttt ttccggcttg attgtagcac catgatacaa aacaggcgca ccattccacg
     4381 cggctgttaa agccgtacca agaccagtaa gggtagccgc ccacatttcc gacttgctgc
     4441 tttgcaagct ccataagctc ttgatttccg gggcgcgtac cgtttacaaa ctccacgccg
     4501 cttaaatcct cggacgggct tccgtcggga gaaccgccgc caaaaatcag cggtttgttg
     4561 ccgcttgttt ccaagtagac atggaacatt tcaagctgct gtggcgttaa aagctccgat
     4621 acaaaagagg aaatcggctt gtttgtcagt ttcacattca agatgtaata ctcataaggg
     4681 acttcctcgg tggttgtttc ccctgtttcc gggtcggtgc ttgtttcggt gcggtagcgt
     4741 atttccacct cctcggtcag cgtcaaggtg tactgcatgg caaagatacg tttaagctct
     4801 gcctgtgcgc tctgcggggt atagctctgt aagagggcgg tcaagtaaga cgctaattcg
     4861 tgggagttat gcccgatact gtcaaggtca tagcggtatt catcatagcc ggggtgggtg
     4921 ctttcgatat tgtcaatctc actttgcagc ccgttttcca atgcagcata gttgttttca
     4981 actgccacaa ggtcgctgtc ctcggacgtg taggacgtgc caagcacccc gtttgtcaag
     5041 cccgaaaaca tagccccgca agaggacagc cctgcggaaa tcataataaa gagaagcagc
     5101 gcggcaactg caatcaaaat gcccgccggg tgtcttgccg caaatgcggc ggctttcctc
     5161 gtttccccgg cggttttctt cgccgtcttt cttgtagctt ccgctgcggc ttttgtccct
     5221 cctttctttg ccgcctttgc atactggcgt tttatctgct gtttctgcat gaaacgggaa
     5281 agcgggttag aagtaagctg cgggttgtcg tgcagggtct tgtgatactg gaagttgacg
     5341 tttgccttaa atgctgcctt ttctgccttt gctgcttccc ggtagggttt cagcttatgg
     5401 ctgcggtagc cctgttttaa cttccgcgcc ccgtatttcg caccgcgctc ggcaagttcc
     5461 tctgatttat gcgccccctc aacgccggaa ttgtcctttt caacggaatg tatcttgttg
     5521 tggacgaaaa tccctgcttc ctgtgcaggg cgggacagcg ggtttccctt tttgccgccg
     5581 ggtatcggtt tttcctgttc ttcaaagcgt aagcgggtct ttgccttgcc tgcggcttcc
     5641 tcaaaggtgc gctccttaac cagtttcttt tgctttggga tagctgcctt tgccgcgtcc
     5701 agtcggtcag cggctttgtt tgatttctca atatagcgtt ccagttccgg ggcggcaagt
     5761 tcttcctcgg taaactgcaa gcgggacgat gttgtttgtg cggtggcttc ctcctgtgcc
     5821 ttgtgtaccg cttttttact ggctctgcgg gtctgtgctg cttcgatatg ctccatgaca
     5881 cgctctgcgg tggcggtgtc ccttgcgggg gaagctcccg gcgcgtgggg caacggggaa
     5941 gtttgggacg gtacgccttg aagctgtgtc ccgtcctgtg gggcttgctg ctccggggac
     6001 ttttgaaaat ctgcttcctg ttcccgttta ctgatacgct cggcggtctg ctgtgtttcg
     6061 tttacctcga taagcccgtc gcgggtcatt ttctgtgtga ttttatcgcg ggatttatat
     6121 tgcttcatgg tctgcacctc actttccgct ttcggaaact tcctctggct tcgttgtcat
     6181 tacccggtaa agctcggtat ctttcgggaa gcggtctaca aaaggcagca ccacatttcc
     6241 atagaagata agcccctcgc ccgcttcggt gtgggtaaca tacttcatct gctgcgggga
     6301 aatgttaagc tgctttgcaa ggatagtacg gtcgccctgc gcttgattga gcatgaggac
     6361 aaaatcgctg ttttcaaaga tattttccac ctcgcggctt gccaaaaggt cgtagggtag
     6421 ggtaaaggcg ccttgccgcc gctggcggca ggtttcctac accctccctc ggaaccggac
     6481 ttacccctct cgaagtatcc ggctccccat tagtaattga cgtattgatt agtgcctgtg
     6541 tattctttca tggcacttgc tgcaaacaac aagtgttttc cgccgcttct ttttcatcac
     6601 ggtttcccaa tcagaattac ccaactcgtt taagttccgt ataacgtgaa cctcatacaa
     6661 gtcggcttct ttacatccac atagttcaca aactctggca ttgagccgtt gtctgatagt
     6721 ggttttccag ctaaacttct ttctctgata gatgatgtca ctcgcttcgc cacgcttaca
     6781 atctgcgatt ttaacagggc gaacacgtct agtccctgct ttagtttcat aagggacaga
     6841 ccatgttttc ccgtctttat actggcgaat gattttgcgg atactggttt tatgcttatt
     6901 ggcaatcgtc tttaagcaac tgtactccat aagatagcag aggtagtcca gcgtgtgata
     6961 atcacaagcc agattgtaat agttgcatag tcctcttgct tcagcattgt actgttccac
     7021 aatctcataa tccgcaaggt tgagaagtcc ggctctgtgt attgtctgga actccccgtt
     7081 ttccttttgg cgaatgactt tctttgcaaa catgaatttt tcaatctttt ccgtatgggg
     7141 aatagacaag gcaactttca tgtgcagggt acgggatttt ctccacgttc cgtttttcat
     7201 tctgtgacct ttgacttcct gatttctgcg gacacaaata tcatagccaa tgaagcgaac
     7261 tttttcagaa ctgtgcgtta tcagcgtctt ttcctcgctc agagttaatt tcagctctct
     7321 gcttagaaat tcggcgattt cggctttcaa ttcctcacat tccgtttttg tgccgcacac
     7381 acctgccagc caatcgtccg cataacggac aaaagtaaac ttcttgttgt cggcaggttt
     7441 gcagggcaga tttctcatag ctttcttctg cgccttatgc tggtcaatca gttcttgtct
     7501 tgtcgcttca tcagcggtat ggtcaatcca ataggacagt ctttttaatt ctttgcttgc
     7561 cgcatgatat tccggtgtct gatatgcaga cctcggctta tcaaagcgtt ctgcgatttc
     7621 cctaaacttc ttgtccagct cgttcagata gatatttgca agtatcgggg aacagatacc
     7681 accctgcgga gaaccgctgt atgtggcatt gtacttccaa ttctcgatat atccagcttt
     7741 caaaaactgg cgaatgatgt tcagaaaacg actgtccttg attttcttgc tcaaaatctc
     7801 aatcaatttt tcgtgattga tgttatcaaa acaaccttgt atatcgccct cgataaacca
     7861 gaccacgcct gtgaagtcag aagaaatctg acggagtgcg gtatgacagc ttttgctcgg
     7921 tctgaaaccg tgggaatggc tatcaaagac aggttcatag atagcttcca aaatcattct
     7981 gacaacttct tgcaggagct tgtcacggaa agacgggata cccagcggac ggagttttcc
     8041 atttttcttt ggaatatact cacgccttac aggattagcc ttgtaggttc cgtcctttag
     8101 tgactggatt aactctttta catagaagtc actgaaaccg tctgcggtat cgtcgtcgat
     8161 accctgtgtt gttgcgcctt tgtttgcata aagtttctga taggcagcgt agtaaatatc
     8221 ttcacgaagc aaatatctgt aaagccttgt gaatacgccg tctttgtgtt cgctggaaca
     8281 cttttcaata cgctctaaaa tttcagatgt tggtttcatt gaggtttctc ctccctttca
     8341 ttcttacatt ttagatgtta ctaactggct cccttcgcca tgtaagggtc attacccctc
     8401 tcggactact atggagcctc cgttgccata tccggcaaat gagtcggact taggcaatcc
     8461 ccagttagca ctgtgcatag gttagtgaag attgtcggat atgctttcgg ttcgttgtga
     8521 cgtgttctca cgcctgtttc ataacgtgtt ggcagttgtc ccgccgtgaa agatgacgtg
     8581 acagcgttat gacatgggtt tcaggacttt tcccacacct gcaaaaaaca ggaactggaa
     8641 ccttacattc gataaaccca atctcatcct catatcttct taacattagg aagcagtcgc
     8701 acctaagtcc tttggtgact attccttttt ctgccatgcg ctgttcccgt aaaggatttc
     8761 tccttagtcg gtaaggcaga ttgtttcgca cactgccgtg tgcggcagta ccttaaactg
     8821 ctttgcacag cgccctatct gggcgcactt tgacgttctg cgtaatggct gtggggatac
     8881 cgccccattt acggaaacgc ttccaaatct ccacgctgta agcggcggtc tgttcctctt
     8941 ttaagagcaa atgaaactcg tccatatagt agcgggtaga tttcttttct gcccggttta
     9001 tcgttaccct gttccatgtc tggtcttgca cgatgagcat acctaacttt ttgagctgct
     9061 tcccaagctg ctttatatca aagcagacaa ggcggttaga aagctccacg ttggttctgt
     9121 ggttgaatac gttaaggctg ccggaaacat acagctccaa cgccgccgcg atacgggcag
     9181 cttccggctc cggctgccct aacagctcgt cgtaaaggtc gcccaagata ggcattttag
     9241 ccgggtcggg gtctgcaaga aacggacggt acacatttct tacagccctg tcaatgacgg
     9301 tcttatcgac aggctgcaag ccctccttgc cgccgataac aagctcgcaa agggaaagga
     9361 taaaatcact tttcagcgca agggggttgt cgtcctcgct gtaattaagg tttatatcca
     9421 tagggtttac atactggggc ttgccgtcaa tgcccctgcc cgtaggggaa agacgtatca
     9481 cttgcccgtt cagacgctgc acaagggaaa aatactcggc ttccgggtcg cagatgataa
     9541 tgtcgtcgtc ggtaatgagg aaagcgtttg tcatttccct ttttgccgca aaggattttc
     9601 cgcttcccgg cgttcccaag ataagcccgt ttgggttttt gagctgcttt ctgtcgcaga
     9661 gtatcatgtt gttagaaagg gcgttcaagc catagtacag ggcttgcccc gtctgaaaaa
     9721 gctcctgcgt gataaaggga atgaaaatag cggtgcttga tgtggtaagc ccccgctgta
     9781 tcgggataag gttttcccca atcggcacac tggataaaag ccccgcttcc tgctgatagt
     9841 caaggcgtgt caggcggcag ttgtatttct gtgcgatacc cgccgcagcg aaaatgtcat
     9901 tttccagttt tcgctttgtg tctgccatgt ttaccacaag gaaagttaaa agaaacattc
     9961 gctcgttgcg gctctgtaag tcctgcaaca agttctttgc ttcgctgcca aaggtggcaa
    10021 ggtcggacgg aattatatcc atgtcatagc cgcttctaac cgcttttttc tgttcctcaa
    10081 tcttcatctt gtcaaggtct gtgattttgc gcttaatggt cttgattgct tccgactggt
    10141 cgatactgcg gatatgtaag ttgacgatta cgcctgtttc caagtccagt atgtcggcaa
    10201 gcatacggtc gttaagctcc ggcgcaagga tttcaaggaa gctcgccgcg ccgattttct
    10261 tccccatgcg gaaatatctg ccctcgccaa aacggaaaga ggacggggcg ataaaatcct
    10321 ttgtagtaag ccctgtcggg gtaagccagt caaaagaaaa ggagaacggc tcgccctccg
    10381 ggtggaatac cccatgcagc acctttaagc gttcatagcc ggataagggg cgggcggtta
    10441 cgccaagcac tttgaaattg ttaagcacgt cggtttcgat acgggaaagc cttgactttg
    10501 cagccgcctt gttatcggct tcaacagtaa aggtaatgta tttgtgcttc acaagcccgt
    10561 tattaccttt actaagctga tttttcagca tatcggaata ctctgtgcgg atagagttaa
    10621 aagcgtcctc ctgtgcgggg atagagattg ctttttctgc ctgttcccgc tgcgtccctt
    10681 gattgatgaa agaaagctgc acactcacgc ttgcgtcaaa gtagttgagg aaatcgcacc
    10741 agttttcaaa aatggcggtc ttatcgtctg cctgtgcaag ctgataattg atgtcctcga
    10801 acacgacgca ctttgaatat ctcttttccg taaccttgca gataccgtcc gggtacatgg
    10861 caaggtaggg aatggtctgc tgtgccgtgc aggctttgcc gtcgcttttt gcctttcgga
    10921 tagcttcggc aatctgtttc ttttcggcgc gggtcagctt acgcctgttt cctgcggctt
    10981 cccgcgtctt ttttgtttgt ccttttgaca atcgctgata cctccttttc taagtttcgc
    11041 tgcttttcca agacagcgta taggttttcg gttttgtaag gtcgctcctt tgggcgggta
    11101 aactttgtct gaatgacgtt tcttaccacc acttcaaggg gctgtccatg cttttcatac
    11161 atggcaagca ggaagcaggg gagcatgacg acaatcatcg ccatagccgc aaggttcgtc
    11221 cctgcgctgc ctttgagcaa aaaaaacagc ggcagtccga ttaagagtgc cgccgcaaaa
    11281 caaacgattt gccgctttgt cagattgaaa gcgacttttg ttttgacttt ggaaaggtct
    11341 tttggtacag gtacatacgc caagaaaaaa cctcctttct gcccttagtg ggcgttgaat
    11401 atggattttg ccagtgtgcc ggatttgaac agggaaaaac agaggataac tgtataggcg
    11461 gcaagggaaa ataccgcgct gtgcagattg tctgcgatta tcatatcgtt taccagtacg
    11521 gcgtaaatac cgacacatat cataatgagg aaaccttgaa aaccaagagc gaatagccct
    11581 tttaagtagt tgtttccaat ctgtccccat tctctgttag tcatagt
//
LOCUS       DAEMHW010000120        11442 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEMHW010000120 DAEMHW010000000
VERSION     DAEMHW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11442
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.120"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            76..993
                     /locus_tag="KPK67_002602"
     CDS             76..993
                     /locus_tag="KPK67_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG2459548.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKTSHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILSEIEKMKKIY"
     gene            1071..1808
                     /gene="cas6"
                     /locus_tag="KPK67_002603"
     CDS             1071..1808
                     /gene="cas6"
                     /locus_tag="KPK67_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG2459549.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYLEELNYITNE
                     VVKNYRGNGLKRKLEFENVGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            1814..3664
                     /gene="cas8a1"
                     /locus_tag="KPK67_002604"
     CDS             1814..3664
                     /gene="cas8a1"
                     /locus_tag="KPK67_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBG2459550.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKKYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTSINELFERNNSLKAQKSESSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFVKENKKIQSIYDYKLKSN
                     VLDLILKSKDLKHLINNTLRNIVKSNLESNNKSNISGLSCFNVVQLRTILNNYKRGVY
                     KMDYKKLRKIYESGCEIHDYYIDKNAKNKIEGITYKILNLIKVGNKNDFMQTILRIFS
                     PSEKIPPEEILQIFVEEDLDFESVGYAFVTGLISGKYEGKDKLPNEKEEK"
     gene            3666..4580
                     /gene="cas7i"
                     /locus_tag="KPK67_002605"
     CDS             3666..4580
                     /gene="cas7i"
                     /locus_tag="KPK67_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBG2459551.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGFADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            4582..5283
                     /gene="cas5b"
                     /locus_tag="KPK67_002606"
     CDS             4582..5283
                     /gene="cas5b"
                     /locus_tag="KPK67_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBG2459552.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            5294..7615
                     /gene="cas3"
                     /locus_tag="KPK67_002607"
     CDS             5294..7615
                     /gene="cas3"
                     /locus_tag="KPK67_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HBG2459553.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTERLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEETENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRSKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEITAVLVKGIEMIHKI
                     GGRFMIMTATMPIIYIDELKKRGVMNSNLAELTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIHENKHTKQIFGKGVLISNEIDQFI"
     gene            7630..8181
                     /gene="cas4"
                     /locus_tag="KPK67_002608"
     CDS             7630..8181
                     /gene="cas4"
                     /locus_tag="KPK67_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBG2459554.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            8184..9164
                     /gene="cas1b"
                     /locus_tag="KPK67_002609"
     CDS             8184..9164
                     /gene="cas1b"
                     /locus_tag="KPK67_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBG2459555.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIIDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            9167..9433
                     /gene="cas2"
                     /locus_tag="KPK67_002610"
     CDS             9167..9433
                     /gene="cas2"
                     /locus_tag="KPK67_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBG2459556.1"
                     /translation="MFVIVTYDIVEARSLNRIRKILRKYLTWTQNSVFEGNITDGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   9617..10501
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9617..9645
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            10691..11119
                     /locus_tag="KPK67_002611"
     CDS             10691..11119
                     /locus_tag="KPK67_002611"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBG2459557.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNAVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYVTKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
BASE COUNT         4919 a          931 c         1933 g         3659 t
ORIGIN      
        1 gaaataaata ataaaaatat caattaatgt tattaggggg ggttactcat tagtaaatta
       61 gctaatacac ttagaatgat gatattaatt caaagcagag gcaaaatgaa atgcaaagaa
      121 ttatcagaag agttggaagt aagtgaaagg caaataaaaa gttacaagat gtatttagag
      181 caagctggta tatttataaa ttcaacacct gggatttatg gtggttatga aatagataaa
      241 tgtaatagca tatcattaat taaattatta gatagtgaag tttcaattct agatatgata
      301 aattcacagt tagagtataa taatgatatt tacaaaaatg aatttaataa tattgtagaa
      361 aaaataaaag cagtattaaa tacaggagaa aaaagcgata catatatgga ttattttaca
      421 gttcaagcac aacgtaattg tgattatgag tctgaaaaaa ataagtgtaa tgaaataatt
      481 agagcatata ccacaaaaca caaattttgg atagaatatt attcattaaa ttcaggaaat
      541 agtgaaagaa ttgttcatcc atatggttta tttaattata aatcagatac ttatatggta
      601 gctttttgtg aaaaaagatt taagtttata gactttaaat tatgtagaat taaagattat
      661 aaggttttag aagaaaaata taatgtagat aaaagtttta gttgggatga atatagtaaa
      721 aatagtatag gaatttataa gggagaagaa attaatgttg ttataaaaac tagtcatcca
      781 ttttcaacaa ttataaagga gaaagtttgg gttaataatc aacaaatcat agaatatgat
      841 gataaatcta taatgtttaa agctaaaatg agaggttatg aggagataaa aagttggatt
      901 ttaagtatgg gagcttatgt tgaggttgta gaaccagata gacttagaaa tgatatattg
      961 tcagagattg aaaaaatgaa aaaaatttac tgattttttc aaataattta tacttggacc
     1021 aatatacttc acggtctagt gttaatatat taattgagag aggtgatatt atgaaaatga
     1081 aaattgaatt tagtacaggt tgtataccaa tatcttataa ttcgctcttt atgagtatta
     1141 taaaagaagc aataagaaaa tctaatgaag attactataa aaacatgtac tattataaag
     1201 aaaagaataa taaaaaaact aaaaatttta cattttcggt ttatataaaa aagtatagta
     1261 ttgaaggtga caattttatt atagaagata aagtaatatt aaatataagt actccagatt
     1321 tagaattggg ttttcatata tacaatgggt taatgacttc taaaaaatgt ttatataaag
     1381 actatgaact tacaagaatt agaatcgatt tatcaagaga aaagaaggta acagaagaaa
     1441 gggtactatt taatgcattg tctccaatct gtgtaaagtc taaagaagga aaattcttgg
     1501 agataactga tgatagatac ttagaagaac taaactatat aactaatgaa gtggttaaaa
     1561 attatagggg gaatggatta aaaagaaagt tagaatttga aaatgtaggt ctcaaaaagg
     1621 tagtagttaa agagagctta agagaattta aaaaaataac aggtaaagag tatcaatata
     1681 ttaatggtta caaaggtaaa tttatattga aaggtgatat tgatgattta aatcttatct
     1741 acaatctagg aattggtttt agacgtgctc agggatttgg agatgtagat attttagaat
     1801 ggaggtgaga taaatgaagt taagagttta tagaggagat tggtttttta atatgggtat
     1861 agttgggttt ttaaatataa taaagaaagc agataaacaa gctgaaatat ttattatgga
     1921 agattatatc gaatttgata gcttatttct tgaaaacttt catgagtatt attttaatta
     1981 ctttatggat gaatatgatg tatcaaaaag aattaaaaaa aatattgatt atagtataaa
     2041 ttttattaaa tctaagcctg atagaatcaa agatggaatt aaaaaaatta aagatagtgt
     2101 gaaacaacaa aatgataaga ttaaaaaatt tgatgaagaa aaatttaacc tgataaaaga
     2161 gaagctagat agtatgtcta agataaaaag ttatgatgaa tttgacagtt tagaagcttt
     2221 agtagatgag actatagata tatttaaaat aaaatcaata aatgacaggc ttacagcaaa
     2281 tctttataaa tatgtagttc aagataatta ttttggacaa gttagttttt ttaatgttgc
     2341 taaggcgaaa ttagatttag atggattaaa acaggtaatg tttaatgatt atttaagaca
     2401 aattatatat tttggagagt tagaagattt attagaagag aatgactatg ataagttgaa
     2461 aaactattta aatgatagat taaatagtat agctaaggat ataaatgaaa aaaaagttag
     2521 taagtctagt ataaatacta tagaaaaaat tatgaaggaa ataaataaaa actttataaa
     2581 gaaaaataaa agcatagaag atataaaaaa gtatatggat agtttagaag tatgtgaaat
     2641 gtgtggttta tataagggaa tattagatga atactcagaa agtaattttg ctccacttgg
     2701 agtaagcacc aataatgcta ggaatatgtt ttggaatcag gcgtatgtac catcaatatg
     2761 tgatatttgt aagctaatat tgttctgtac tccagcaggg gctacttata caagaaagaa
     2821 ttatttaatt aatgaagaaa atgagtttta tttatttgtt aacatggata cttctataaa
     2881 tgagcttttt gaaagaaata atagtttaaa agctcaaaaa agtgagtctt cagattccaa
     2941 agatgaaaat ccttttaatc agcttataaa aagtatagta gaagaaaata cattaaaaag
     3001 tgaatggcaa cttcgtaata tactttttgt ggaatttaaa gcaagtatag attctaaaaa
     3061 atgtaaaata aattatttta atattccaac ttatttagca aagttttttg taaaagaaaa
     3121 taaaaaaata caaagtatat atgattataa acttaaatca aatgtgttag atttaatttt
     3181 aaaaagtaaa gacttaaaac atttaattaa taacacttta aggaatatag ttaagagtaa
     3241 tttagaaagc aataataaat caaacatatc agggttatct tgtttcaatg ttgttcaatt
     3301 aagaacaata ttaaataact ataagagagg agtgtataaa atggactata aaaagttgag
     3361 aaagatttat gagtcaggtt gtgaaataca tgattattat attgataaga atgctaaaaa
     3421 taaaatagaa ggaataacat ataaaatttt aaatttaatt aaggttggta ataaaaatga
     3481 ttttatgcaa acaattttaa ggattttttc accaagtgaa aaaataccac cagaagaaat
     3541 tttacagata tttgttgaag aagatttaga ttttgaatct gtagggtatg catttgtaac
     3601 aggattaatt tctggaaaat atgaaggaaa agataaacta ccaaacgaaa aggaggaaaa
     3661 ataagatgaa aagagggtta acatttacag taatagcaca ggctcaaagt ttaaactatg
     3721 gggaagggat aggtaatata tcagaattaa aaaaattaag tagagatgat ggaaatatat
     3781 atacatttgc atctcgccag tgtttaagat atgacatagt acgattagca gcagaattgt
     3841 ttaattggaa tttacaggtt gtcgataaag aaaaaggtac tattcaattt aaagataata
     3901 taagtataag agaatcagaa gaaatggatt tatttggata tatgaaaaca aataaaaaag
     3961 atgagaaaga taaaaaagga ggttctttga ctagagaagc tactgtaaga ttaagtaatg
     4021 cgatttctct tgagccatat agaagtgata tggatttttt aaataataaa ggatttgcag
     4081 atagaatagg agaacatcct aatcttgcta acatagagca acatttaagt tattatacat
     4141 atacagtgac tatagattta tctaaaatag gtaaggatgg agatatagaa cttgataata
     4201 aagagaaatg tagaagagta gtagaatttt tagaaataat aaaagtattg aatagaaata
     4261 ttagaggtag acaagaaaat ttatctcctc tatttgtagt aggaggagta tatgaaatag
     4321 caaatccatt tttcttgggt agaattaaat taaaaggaga taaaaatgga tttaaaataa
     4381 ataagcaagc tatagaagaa gtaattcaag gtactttttt aggaaaagat ttaaaagaat
     4441 ttacctatgt tggaatggta gatggggtat ttataaataa agaagaattt aagggattgt
     4501 ttgaagataa ttttttaagt gtagataaat tttttggaca actagttaaa gaagtaaagg
     4561 aatattatgg ggtgaattaa tatgaaggtt ttgaaattag atttatttca agaaactgca
     4621 tgctacaaaa aaccatttgc tatgaagatt acagagacat atccactgcc tccatattct
     4681 acaataaatg gattaattca caaaatatta aatgctacag aatatatacc atttaatata
     4741 agtgtacagg gaacatatga aagtatattt aataattacc agacaacata tttttataaa
     4801 aaagatagta ttacatctat gccaatgaat agtcatatgc tattgaatgt aaatttgata
     4861 attcatatag gtggggattt tgatttattg gaagaaatat atgatagttt attaaattat
     4921 gacgagcact tatcattagg tagaaaagaa gatttagtaa gaattaatga tattagattt
     4981 gtagaagtca ataaatttga agtagatgag gatatacaat tagatgatta tgaaaattat
     5041 aagttatcag aatgtaatat aagaatgcct atatatatac caaaaacaag tttacaagat
     5101 acagatatag atggtataag ttacagactc aataattact atgataataa tgctgtggaa
     5161 gataaaaaaa gggtttggaa taaggtagat agttattatg ttgaggatgg aaatactata
     5221 tcaattggag atatattatt agataatgaa ggagatttat tatattttaa taatataaat
     5281 taggagggag agtatgttat atgctaaatc taatccagtg gaaacactta gagaacatac
     5341 tgatgaactt ttaaaacaaa tgaatgtatt aagagaaagt tatggaaaaa atataaatag
     5401 cttagatttt ctagaagagg agatattttg gcaattactt gactttgtta tagaatttca
     5461 tgatattgga aaggcatttt caccgtttca agagttaata aaaagcagaa tggatactaa
     5521 aataaatgaa cctaaaatag taactcattt agaaaataat gtaggccata attatctttc
     5581 tcctgcattt attgattata gttatataga caggaaaaaa aataaagaac ttagagcagt
     5641 attaaatcaa gttattgtat atcatcatga aagagatatc tttatagata aagattttaa
     5701 aaatttaata caaaaaatat tagatgaaga tttaataaat aaagtttatg aactacaaca
     5761 tgaatttaaa gttagatatc caataaaaac agaaagattg agtaaagtat atctacaaag
     5821 tgtagaaaaa agaatagata aaaatcacaa gtactataat ttatatatta tgttaaaagg
     5881 aatactacat agactagacc atagtgcttc tgctcatgaa gttgtggaat gcaataatgt
     5941 tataaatata ggagaagaga cagaaaatta tttaatgaaa gagtttggta gtttgagaga
     6001 ggctcaatca tttgctaaat ctaatagaaa taagaatata atactgattg catctacagg
     6061 aatgggaaaa acagaaactg ccttaatttg gatagataaa gacaaggcat tttttacact
     6121 tccattaaga gtaagtataa atgcattatt tgatagatca aaaaatatag ttggagttgg
     6181 agaatcaaat gatacattct taggtttgtt acattcaaca gcaatagatt atctagaaga
     6241 aagtaatcaa gaaaattcaa gtgagattgt agatttagca aagttattat cttgcaaact
     6301 gacattttct acaatagacc aaatatttaa atttcctttt ttgtatagag ggtatgaaaa
     6361 agtgtactca acattagcat attcaaaagt agtaatagat gagatacaag catattcacc
     6421 agaaataaca gctgtattgg taaaaggtat agaaatgata cataagatag gtggcagatt
     6481 tatgataatg acagctacta tgccaatcat atatatagat gaattaaaaa aaagaggtgt
     6541 aatgaatagt aacttagctg aattgacatg taatacagaa aaaataagac attgtgtttc
     6601 tattgtagaa aattcaatag atgaaaactt aggtaaaata atacaaagtg gaatgaataa
     6661 aaaagttctt gtaatagtaa atacagttaa aagtgctgta gaaaaatatg agttaataga
     6721 agaaatagtt aaaccaaaag gaatagatgt caacttaaat cttttacatt ctatgtatat
     6781 acaagaagat agagcaaaat tagaaaaata tataaaagaa tttgcagata gtgatagtaa
     6841 tggtatttgg attactacac aattagtgga agcatccctt gatattgatt ttgatgagct
     6901 tcatacagaa aattcaacac ttgacagtct atttcaacga tttggtagat gttatagaag
     6961 tcgtgagtat gaagaaaata gcccaaatat atttatatat acagaaaaag ctacaggaat
     7021 aggtagtatt tatgataagg atattgtaga aaaaggactt gagctactga aaacttttat
     7081 aaatggaaaa gaatgtgtga agatgaaaga aaaatataag gtagaaatgg ttaagatact
     7141 atactctaaa gaaagtctag aaggtacagc ttttgaaaag agatttacca gtgctttaaa
     7201 tattttagat actataacgc catatagttt aggttctaaa gatgcacaaa atatacttag
     7261 aaatattgaa ggatatacag taattccaga ggatatatat aataaaattg aagaaacttt
     7321 aatcaaagat tatgaagagt tgggacaaga gcttataaat gcatatagta atgatgatag
     7381 acaattgata aataaaataa agtctaagag aaaaaaagca agaagagaga taattaaaaa
     7441 aactgtaaat ttaccaatat ataaagctaa acaaaatgta attgacatac aggtgaaagg
     7501 tcttgaagat ttaaaaatac ttttatataa atatgatata cacgaaaata agcatacaaa
     7561 acagatattt ggtaaaggtg tgcttataag taatgaaata gaccaattta tttgattttt
     7621 atattaacaa tgtggctaaa atttactaaa gaaggtgatt ttgtgccaat agatttagaa
     7681 gtattaaaag ttcaaggtgt aaaaataaat tattattatg tatgtaaaag aaaactatgg
     7741 ttattttcta aaggaataac catggaacaa aatagtagta gagtattgtc tgggaagata
     7801 gtacatgaag attcatataa aagaatgaaa aataaagaaa tattgattga tgatatcttg
     7861 aagctagata taataaagga tgagtatgta aaagaagtaa aaataagttc aaagatgaaa
     7921 gaagcagatg aaatgcagtt atactattac ttatactatt taaagaatag tatgggtata
     7981 gagaaaaaag gaactataaa ttatgttaag gaaaagaaac aagaagaatt gatattgaca
     8041 gaagaaaaag aaagaattat tgaaaacaca cttataaata taaatgaaat aaatatgcag
     8101 ttatctcctc ccaaaattga gaaaaaacat tattgtacaa aatgtgctta ttatgaattt
     8161 tgttttgtga aggaggagta acaatgtata aagactatta tattataagt aatggggata
     8221 taaaaaggaa agaaaatacg atttactatt acaatttaga aggtgagaaa aaaatattac
     8281 cagtagaaca agttgatgat attcatatat atggagaagt agatttaaac acaaaactta
     8341 taaattacat aagtaaatat ggggtgatgc ttcatttcta caattactat ggatattatt
     8401 caggtacata ttatccaagg aagaaaaatg tatcaggatt cagtttagta tgtcaatcag
     8461 cctgctattt agataaaaat gaaagattat acttagcaaa atcctttgtg gaaagtgcat
     8521 cataccatat tttaagaaat atgagatatt acaaagttga tgaaagttta attgatacta
     8581 taaaggatga actaaatgat ttgaatgatg tcaaagaaat atcagaactt atgggtaaag
     8641 aaggcagaat aagaaaaaca tactataaat catttaatca aatattaaaa gatgattttg
     8701 aattttataa aagagaaaaa agacctccca aagacccagt aaatgctctt atgtcttttg
     8761 gaaatagcat aatgtatacg aatgttttaa gtgagatata taagacacaa cttgacccta
     8821 caataagctt tttacatgaa ccatcaacaa agagattttc tttaagttta gatattgcag
     8881 aaatttttaa gccaattata attgacacaa taatttttag cctgattaat aataaaagaa
     8941 ttaataagaa tgattttgaa tatcaagaca aaatttgtta tttaagtgaa aaaggaaaga
     9001 agaagttttt gcaagaattt gaaaataaga tgcagacaac tataaaacat agaaatctaa
     9061 atagaaaagt ttcatataga atgtttataa ggttagagtg ttataaatta attaaacatt
     9121 ttataaatga tgaaaggtac aaagttttaa aggtgtggtg gtaagtatgt ttgtcattgt
     9181 tacttatgat attgttgaag caaggtcgtt aaatagaatt agaaagatac ttagaaaata
     9241 tttgacttgg acgcaaaatt ctgtttttga aggcaatatt actgatggaa agttacataa
     9301 atgtatttct gaaatagaaa atattattga taatagcgag gattcaatct atgtttatga
     9361 gataaaaaat cctaattcaa ttaaaaagaa atgttatggg attgataagt attctgatga
     9421 aatgtttata taggtttgca gtgagcgata tttatgctaa aataggtgtt aacagttgga
     9481 atataaggga ttgaaggtgt atgataactg ttatcaattg cactactgct cgctcactgc
     9541 aaattttgat gtttttattg aattataatt gcttgattga agtattttca atgtattcaa
     9601 ttatacctat tttggggttt tatattaact aagtggtatg taaatacagc tttacttttt
     9661 ggtcgtataa tacttcttat agttttatat taactaagtg gtatgtaaat ggttataaat
     9721 gaacttaaac ctaaagcgga ttatacaggt tttatattaa ctaagtggta tgtaaatctt
     9781 aaaaactata acgaatgtaa ttactcttat tctacgtttt atattaacta agtggtatgt
     9841 aaatattttc atgtgtttga tgctcatgac tgataacaaa gttttatatt aactaagtgg
     9901 tatgtaaata gtatctaaag caataagtag ttaaaactag atattagttt tatattaact
     9961 aagtggtatg taaattcaat aacaaaaatt gattgtaata ttagacttat tgttttatat
    10021 taactaagtg gtatgtaaat gtgtcagctc catttttaag aacattaaac atttcttgtt
    10081 ttatattaac taagtggtat gtaaattcac ttgatggtag ggtgtgctga acgtttagac
    10141 tcgttttata ttaactaagt ggtatgtaaa tttttacaca agaagaaaaa gatattcttg
    10201 aatattggtt ttatattaac taagtggtat gtaaatagct gaaaaagttg caaacatagc
    10261 agaaagagga aacaagtttt atattaacta agtggtatgt aaatcaattt atataaaata
    10321 ctatgttcaa gtttaggaac agttttatat taactaagtg gtatgtaaat aaaaaatggg
    10381 ctgatgaaat gggtataaca agagaagttt tatattaact aagtggtatg taaataggat
    10441 aaagaaaaga ctcacacaag acacagtgtc aggttttata tcaactaagt ggtagataag
    10501 tagaatatta gcaatatcaa cgagtattta gaaacttatt ttatattaat aaagcagtat
    10561 ataaaattgt agaatcaaca atagcatata ctaaaacatc caccttatat taactaaatc
    10621 atatctaaat aaaatacact tacctacaaa cattataaaa tcaatacaaa aatgaggtga
    10681 aacaaaattt atgataaaga aattaaacaa taaagatata aataaaatca tggaaatatg
    10741 ggaaaaaagt acaatcaaag cacatgactt tataagtaaa gaatactggc aaaataacta
    10801 taatgctgtt aaaaacgaat atatacctat atcagataca tttgtatatg atgatggaga
    10861 tgaaataaaa ggatttataa gcataataga taaaagcttt ataggagctt tatttataaa
    10921 gcccaaatac caaaatctag gtatcggagg taaactttta gattatgtaa ctaaaaaata
    10981 taaaagtcta agcttagcag tatataaaga taataaaaaa gcagttgtgt tttataataa
    11041 aaaaggtttt aatatagtaa aagaacaagt aaatgaagat tcaggattta aagagtacat
    11101 aatggaatat agtaaataat atgattacat tctattaaaa ggataattaa tttattttct
    11161 atttgatttt tataagagta atttttaaaa tagactatat gaagataaag atatttatta
    11221 tagatgtaag ttaatcagaa ttgatatagc tacaaaagag gaaaataaaa ttgtatagtg
    11281 tatttcagat ttaacaaatt tactatttaa agataataag tttgatataa tattaaagat
    11341 actttcacct ataaactata gggtatttga aagaatttta aataattatg gaatgctaat
    11401 aaaggtttgt taaaatttaa taattattag attatagaaa at
//
LOCUS       DAEMHW010000121        11441 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEMHW010000121 DAEMHW010000000
VERSION     DAEMHW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.121"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            409..804
                     /locus_tag="KPK67_002612"
     CDS             409..804
                     /locus_tag="KPK67_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459558.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            847..2946
                     /locus_tag="KPK67_002613"
     CDS             847..2946
                     /locus_tag="KPK67_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBG2459559.1"
                     /translation="MNFYIKHEIRGRIRFDLQLSKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTIGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLCMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIILKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(3171..8381)
                     /locus_tag="KPK67_002614"
     CDS             complement(3171..8381)
                     /locus_tag="KPK67_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG2459560.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKRLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYKLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYIDGGLGGNGQDGK
                     KGDTGITGEAGANAKISSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDDSDRNYYYFDRYVFNHALNQKQAADDGEKLNALYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEDISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNRKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILKSLGYIDFKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(8396..10891)
                     /locus_tag="KPK67_002615"
     CDS             complement(8396..10891)
                     /locus_tag="KPK67_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG2459561.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEISSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIER
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGEN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
BASE COUNT         3678 a         2073 c         1333 g         4357 t
ORIGIN      
        1 aaatttatat aaattatata agctagataa agtattacct caactatata agctagatat
       61 ataaattaca tatataaact agatatataa actagatata taaactagat atataaacta
      121 gatatataaa ttgcatatat aagctatata agaattattt caactatata aactacatat
      181 ataaattaca tacataagct agatatataa actgcatata taagctatat aagaattatt
      241 tcaactatat aaactacaca tgtaaaattc atgtaaattt actaatataa atcaaataaa
      301 attactaaac tctctaaact aattttgtaa aacatactct atttttaaca tttgttacct
      361 tatacataag tacaaacaaa atctaactaa atgaaaggat aatgtaaaat gaatatttta
      421 aaaatgaaaa aaacagggat atttttagga ggagtacttt ttggtactgt aggtgttaag
      481 atactttcaa gtgattgtgt taaaaaaact tgcactcgtg caacagcagg agcattgaaa
      541 gttaaagatt ctgttcttga aacaactacg aaaattcaag aaaatgtaga agatatttta
      601 gctgaagcaa aagaaatcaa cgagcaagaa gacttatcta cttctaaggt aaacgttgta
      661 caagatactc ttaaatgcga acaagaagtt cttaaaactg aaactataaa aactgtagaa
      721 gatactaatt ctaatagcat taaatgtgaa gaaatcaaag ataacgtaat tgaagaaaaa
      781 aatagtactt gtacaacaga ataaatttaa tgattattat gattaataat atcaggagga
      841 agtaaattga atttttatat aaaacacgaa ataagaggtc gtatacgctt tgacttacaa
      901 cttagcaaat taaacaacaa gcagtccgat actctcttat attatttaac ttctataaat
      961 ggtattacca aagcaaaagt ttacgataga actggtgatg cagtcattta ttacactggt
     1021 gaaagagaat acataataag agaaatcact aaattcagtt ttaacaatag caaaatagaa
     1081 aacctagttc ctgaacatac cagcagggaa ttaaacaatt attatagaga caaattagca
     1141 aataaactgc tagtcaaaag tatctccaaa atattttttc caaagtcctt aagatttttt
     1201 ttaattggac ttcattctat aaaatatttt aaaaaaggtc tctcttccct gctaaataaa
     1261 aaaatagaag tttctgtttt agatgccact gctataggta tttctatttt tagaaaagat
     1321 atcaatacgg ctggttcagt tatgtttctt cttggtatcg gtgaactatt agaagaatgg
     1381 actagaaaaa aatctataga cgacttggct caaagtatgt cactaaacat tgaaaaagta
     1441 tggttaagaa aaggtgacac tgaaatccta attccaattt cagagataaa ggaaggagat
     1501 ttagtaagtg taactatagg aaatatgatt ccattggatg gagttatagt atcaggagaa
     1561 gcaatggtca atcaggcatc acttacaggt gaatcccttg cagttaacaa aaaagaaggc
     1621 tcttacatat atgctggaac tgtaatagag caaggcaata ttgttatgtg cgtcaaggaa
     1681 aaagctggta ctactagatt ccagaaaata gtaactatga ttgaggaatc tgaaaaacta
     1741 aaatcttctg tagagagtaa atttgaacat ctagcagata cattagttcc atacagcttt
     1801 ttaggaagta tattaactta tgcaattact agaaacccta taaaatcact ctctatatta
     1861 atggtagatt tctcttgtgc attaaaactc tctatcccta tttcagtact atcagccatg
     1921 agagaatgta acaataacaa cattactgta aaaggtggaa aattccttga aggtgttgca
     1981 tgtgcagata ctattgtatt tgataagaca ggcacattaa caaaagctca accaactgtt
     2041 tcagatataa ttacattcca aaattataat aaggaggata tgttaagact tgcagcttgc
     2101 ttagaagaac acttcccaca ctccatagca aatgcagtag tttacgaagc tgaaaaacaa
     2161 gggcttagtc acaaagaaat gcatacagag gtagaatata ttattgctca tggcatttca
     2221 acaaaagttg atgataaaaa ggttgtaata ggtagcaaac attttgtatt tgaagatgag
     2281 aagtgtacta tcccagacgg tgaggaggat aagtacaata atctatcaga tgagtattca
     2341 cacctatgta tggcaatatc aggtaaatta tcagcagtta tttgcataaa tgacccttta
     2401 cgaaaagaag caaaatatgt aatttcaaat ttaagagaat gcggaataaa aaagattgtg
     2461 atgatgactg gagatagcga aaaaacagct aaatctatag caagtaaagt tggtgttgat
     2521 gaatattatt cagaagtctt accagaagac aaagccaatt ttgttaaaag tgaaaaactc
     2581 aaaggtcgca aagtaattat gattggtgat ggaatcaatg attctcctgc tatttcagaa
     2641 tcagatgtcg gaattgcaat gagtgaaggt gctgaaatag caagagagat ttcagatatt
     2701 accatatcag cagataattt gaataattta atcattttaa agcagattag taataagctt
     2761 atgaaaagag ttgatttaag ttctaagttt attattggct ttaatttagg tctgatttta
     2821 cttggtgtag gtggatttgt tagaccaagt acatctgcct ttttacacaa cgcatctact
     2881 gtcggaataa gtttaaacag tatgacaaat ttattggaaa acaccaatac ttataactac
     2941 cattaatctc cattacataa aacatatgat ataaaaaatt tataattcat cttttaaaaa
     3001 aaatatgtcc taaaattagc ttttttctaa tcaaaattat ataaagcata ctttacaaat
     3061 tttaaactca acttatttct attttttaca aaaaaggaga tgattcaaat tttcattttg
     3121 aaacatcccc ttttgtaaat ttctttaaaa tttatatatt aattatattt tcaaccaata
     3181 attgtatttt caaccaatct ttcttcatag cattttaaat cattttgctt tagataatta
     3241 aattcagact ctacataaat accacagcct accaattcaa ttgtcaaatt aggtatattt
     3301 tccttcagta tactagtctg ctcataaata attctttcca ataaatcaac ttgtaatata
     3361 gaaaaatgct tcataatctc cccatcacta tctatatctt tcttgatttc ataccaactc
     3421 tttgacaaaa ttcccttaaa atcaatatac cctagactct tcaatatatc tatactatcg
     3481 ctccatgcat acttactagg tttatcaaag tatgattcta gtttcttctt ctgctcattt
     3541 gtaagtgaat cataagaaag cttcctatta aaatgtgcct tcaaagaaat cttagccata
     3601 gaatatggac ttctaccaga gtttaatcct gcatttttga acttttcaat tcctttatct
     3661 tctactgaag accatattga gtttattgaa tctgaatcat tgtatttaac atcaagacca
     3721 tgtgtaatat ttatatagtt ttgtggaaat acatattgat aatagacatc ttttttatca
     3781 tcatatagag agaagtatcc actatcatat aaatgaatat gcacctttac ttttaaacta
     3841 tcctctaaaa tatttcgtct aagattcggg atatttgcat acatggttac atattttatg
     3901 gcatcaaagt ttttatttaa tggaacttca aaactaattt catctcctgt caaaagcttt
     3961 ttcacttctt cttcattaca atcatataga gaatatgtat tttcaaatcc cacacgtgat
     4021 ttcccatcta aactgcctgt taatgctacc ttcaaaatat ggttatctgt tttttgccat
     4081 tggtaagcat cttggtcaag aaactctcta atctgtctat tttgctcttt cgcctgattt
     4141 ataacctcgt tttcatcttc tgtaatccct acatggcatc tatttaagat atttactata
     4201 ttgcttccaa atgggaatgt ccatagttga aaagctgctg cctcaacctg acacaagtat
     4261 ctaacctgat ttaattcaat ttgatggata agttttttct gatattcatt catctgatta
     4321 ccactatctg acatttgata aattaattct cccattgcaa gattatcttt tttcttatca
     4381 atcttatcag taatgataat ctgagtttcc atcatatcct gtactttttt gaagattata
     4441 gaaatgtcct ctgaattttc catttttaag cttccaaact ttgtgcttaa ttcagttaaa
     4501 acttccttca tctttaattt acttatctca ttttcaaaaa tatcttgatt tttaactgta
     4561 ctatcgaatt tttctacaaa tggtataaca gattctttca aagttttatc tttaaattcc
     4621 accatttgag tattcaattc atctaatttc ttaatattat tttctttatc tttaattgcc
     4681 tctttaacta cctctatttt actctcaaaa ctagatttta cattaagaca attattaaca
     4741 ccactaatcg cagtatttat aagagaacaa gcagaacttc caatactcaa tgcattttta
     4801 attttagatg tcttttgctg tctttcaata gcttgtttac ttttctctag tgcagctaac
     4861 ttatcttttt gctgtttatt attgtaattt gcctccaaca tacctatttc ttttttctgg
     4921 atttttgtgt aaatttcctt atactcgctt tcacttaacc cctcttttgc tctttgaata
     4981 tcgtcagctt tgtctgcata gttatcatca tctatagact taactataat ctccttaatt
     5041 tggtcaaatt tagttcttct ttcttcatca acacctgcca agttataagt cattttatct
     5101 aaagcatttt ttctatcttc acttagctgt tctccaagta caaatctcat accatcagaa
     5161 tccaatttaa aatctgaaaa atcaaccatc ctgttggtcc attggtattc ctcttcttta
     5221 gctgaaagat tttccttcaa agattttgca ctgtcaaatg cccctagaca agcttgacca
     5281 acaccactaa atgcagtcag taaactcaat gcgctttgta ttccagaaag aattaactgt
     5341 gttttcattt gttcctcaag ttttgcctgt ttctttttta gttcctctat gtcttcttgt
     5401 tcatcttctt tcatctttat aatttcttga atcaacaggt tcatttttgc attaacagaa
     5461 agcattttat tctcaagttc attttttaaa acctctattt gttgatttcc agattcaatt
     5521 tgagttagga tactttcctt atatgtattg aaatatcctt cttttaactc atctttcttc
     5581 ccttgttcca attctcttaa ggtcttatcc agataatctg tcaaagaacg aatatctgta
     5641 acacgatttc ctccaatttc actttcttca atcatctgca cataaaatgc tgtcttctgc
     5701 tcaagtgcaa ataaaatcat actccattcg ctaaaattct gattttttcc tacaaaagat
     5761 tctattctat cagactgcat caatttattt ataaggtatt catagatttc ctttgcctct
     5821 ttataaaatt ctttctttgt attcatatca tccagagaaa gtactgcttt atttttcttc
     5881 tcaaaactat agtacttctg aagtatttct ctatttaatt gcatctctat tttctcaact
     5941 gcataaaaaa tttcataaat atgtgaaagt tctaaaatat cagcttctaa aattcctact
     6001 gcacacaaca gtctaagatt tctataacct ttaattccag aaaaaccaaa tctctcgaaa
     6061 aattcataga agaatgtatc tattgacaaa gaatactcct gctttaacaa ttccttttct
     6121 tttagtgcat ctccagagtg accggctttt ccctctgctc cggctgaacc tgaatcttca
     6181 cgtttgctaa ttgtccagtc cttatgttct tcataaagag catttaattt ctctccatca
     6241 tctgctgctt gcttctgatt taaagcatga ttaaacacat atctatcaaa ataatagtag
     6301 tttctatcac tatcatccat tggaggagag gtacctatat atcttattgt ttcttcatat
     6361 atacctctaa ttccaccaag acctcctgca ccaccagctc ctccagagcc tccttttcct
     6421 gctaaaccat gatttccatc ttttttactt atagcacttt ttaataaagg agctttccca
     6481 aggagataaa cctgtccaga cttgcttcca agacctgcat ttccaccatt acctcctgtg
     6541 cctccagttc caccttgttt tcctttttct gcttttacca catagtattt tgtaccttta
     6601 acattagcaa attcttttga ctctttttta gaacttattt tagcatttgc cccagcttct
     6661 ccagttatcc ctgtatctcc tttttttcca tcttgtccat ttccaccaag tccaccatct
     6721 atgtaaagtt taagttctcc tcttttttct atttctttaa aagataatat gatatcacct
     6781 gaagtacctg cattatttcc atttcttcca ttcattccgt cgtaaataga actttctcca
     6841 accaattttt ctgcatcctt accagatacg ttgattgaat aagttccttc tggtatatgt
     6901 aatttttctg taagtagtac aatattctta cctgagaaga cttctttctc catattacag
     6961 tcaattttca actcacaatc tccatacata tatatagatg acaaaatatt aagtttataa
     7021 ccagatatta ttttagtcaa atccgataaa ttcactttag gtataggaat atatatagaa
     7081 agtacatttt ctttctcata tgattttata tatgtacaac tttgaatctt ctctatccta
     7141 tactctgcat cttcaagcct tttggcttct tcttccctat attcttcttc tgctatccaa
     7201 tatttgatat cttttaatgt atcttctaaa gtatttttta gcaatgattt taacaactca
     7261 cttagaccaa caatatgagt ctcactacag taactattta actctaaaag aagacgtgtt
     7321 tcttctaata tattcacttt ctgtacgcca tcaccatatg aaagttcttg tacaaactcc
     7381 ataagctctt tcatattatt agtcctacta cataaatctg tatacatctt atctaattga
     7441 atctttttat cttttagact atttatagaa gatagaatgg aaatagaatc ttccatacct
     7501 tttttaacac tttctatata aggaactaaa agtttttcta ggtctttata ggttacatca
     7561 acagcttttt gtatttctat tttacaagaa gcagtaagca tcaaccccaa atctgttttt
     7621 tcaaatgttt caggtagatt tttcatttca tccaacaact tttctggctg tttatctagt
     7681 ggattacata atttaataga tacaccatct gataatgcta catttaataa gtacactaat
     7741 tccttacaac cgtcgtaatc tttctcttga ctatctaggt cttcttttgc tttttccaat
     7801 atatttctta ttttttcttc tgttacatta ccgattagtg ttttaggtat tttattgata
     7861 agaaagaaga aattttcttt atactttgca taatcaccaa gcatatcttt caaatttttg
     7921 cctgtttcta tcaataatgt atgtctagag tccagtaggg attgatagtc taaaataact
     7981 accatccctt ttacctttga agatgtggaa atagtttccc ttacaagcat agcaccagca
     8041 atcatatact ctgctccacg gttatcaaaa aaacctgggc aatcacataa tgaaaaatct
     8101 ctatttgcaa tatctacaac ttctggacat aaagtcttag aatatacacc atttccaatt
     8161 tcgacagctg aatcttttgc agttatatat atttgtccag tctcttcatc cgttttttct
     8221 tccatcacac agccttttaa ataatttata caagtactct ttccaacgcc tgaagcacct
     8281 aagaataaca aaattgactt ttcttttaag tcatattttc taagctcgtc tcttccttct
     8341 tccaaaaggt ttacgatttc actataaagt ttatttgcca tacatccatc cctttctata
     8401 tttcggaaca atagccttga tatttttctt taataagatt ctcttctacc tttgaaacag
     8461 ctgttgataa cttagtgttt ggagcattaa atcctccatt ttctcctcta tctccatatt
     8521 cagcgtattc tcttgctggt cctgttttac cttcattcca gcttccacca ctaaattcta
     8581 tatgatattt aattatacta tctaacttat aatcccaact tgtaaaatat cctgtatagt
     8641 atacagtctt atctatacca cttggagaaa atgaaagtgt attatatccg tgatagccac
     8701 cacttcctcc atatccacct aaacctggtc taccatttgg acctgcttct ccatctttca
     8761 ctgttatatc acaatgatta attattctat ttttttgttc ctctgaatct gaaatgaagt
     8821 aaatattacc tttcttacca gcatttccac ctcttccagc atttccacca gctccacctt
     8881 tttcaccttt tttgccactt gtaccttttt caaaataccc tttaaacatc tgtccgtgga
     8941 aataacttgt atatcctgca tcagctccat tacttccaga agctcctgtt tcaccatttt
     9001 ccccatctat tcctttagaa ccatttcctc catttgcaat cagtgttaga cgataatttc
     9061 taatttcttc tttaatatat aagtaaatat tacctgcatc tctaccatct tcaccatttt
     9121 tcccatacat agaattatct acaagttctc cgctctttcc acttacatta atttttacat
     9181 tactacctac atcaagattg ttagaagcaa gaataatatt tttctctgat gcaaagtcca
     9241 ttccaaggtt atcatccata taaatagtat ctttagcaaa gaacataaat gtagaaacat
     9301 tgtcatctac ttttctattt ttgaaatatt ccaatacact gctaattact aagtttggaa
     9361 ttaatgctct tatttcaata cctttcttat cattaatatt aattgttttt actataaatg
     9421 gatttaaaat gtatttacca ataaattctc taattatctc attaccttct tctccatatg
     9481 cacttaatct tgttatactg tcttctaact cttcattttg tgtaactgta acatttgtca
     9541 aaattgaatt aaaatcactt acactatata cataatcctt tttatttgca acttccatta
     9601 atttttctct atttggtaat gtaaatacct gtttttgcac ttcataagaa actaaaactt
     9661 tttctaaatt ctttcttatt ataatgaagt tttgttccat tatcttctta cttgcctttt
     9721 ctataataaa ttccatatgt acagaaggta ataaaatttc cccagttcca tgttctgtat
     9781 atttacttaa agcatcaatt atatctaagt ttccttttat cttattttgt aatttcatgc
     9841 aactatcttg taagaataag ccactttgtt tggctaatac gttgataaat ctctctatag
     9901 taagacttgt tgtactttcc aaccagtttt taaatacttt tacaacctca tcatattttg
     9961 ctatttcttg aatcattgtt atacttccaa taatattgat aaaatcttct gtatacgcct
    10021 taacataatc ttctaggcta gatacgatat tgttttgaat ttcttttatc aacgtatcta
    10081 aaactaatct tgcatctgca ctaattggat attggaatgc tgatgtctta tctttaactt
    10141 catctaattc tagtacttgc tttaataagc cttccctctc atctgatgca agtggcttac
    10201 aaagatgaat attttttcca ttatctttta gcaattcttc aaataaccaa gctttttcct
    10261 ctatattact gtctaaattt tcgcataatt gttcatattt tctttttata acattatata
    10321 tttgtttttc tgttcttcct aagaaacttt ttgaaatgaa aaaagttata ctacccttaa
    10381 aattttcttt atgaagacac atatttaact gttggaatag gtctagtact tttgtaagtc
    10441 tagaatctaa tatatctgct tctggtatca atacaattac tgcacatata gatgcagaat
    10501 ttttaaatac ttcacctaat gaaactgctg cactaatatc atgggctact cccctattgt
    10561 cgctaaaacc tgcagtatca cataattttg catccattcc ttctattggt tttctcatat
    10621 ttacaatgtt cgggtataaa gtttgagaaa cagctcctga actaatctct gccacagggt
    10681 ctaagcaatc aatatatgat ataccagtat cttcatcttc tacttccatc attacacatc
    10741 ctgcaagata gttaattgca gtacttttgc cacatcctgt cccaccaaga attagaatta
    10801 aattttgatt ttcagcctta tttattgcta cttttccttc atttaataag agtgttattt
    10861 cttctaaaag ttgattgtta ttttctggca tttattttcc cccttggtta tatattttat
    10921 ttaaataaag tttaatccct taagtagatt gcaaagtttt tagactatat ttaaatttgt
    10981 tttttatttt ttatttttaa taaatttgct ttttcactca atttgctttt tcactcaata
    11041 attattcttc tttgtttatt aaaataactg tgttgtataa tataatcttt accaacacag
    11101 ttattttatt taatgcgctt attttattgt attttatgta atacagttgt tttttatatt
    11161 atatgtccat tattttccta tggttattac atatagattt ttaattatat ttgtaccatt
    11221 aatttaaaca ttcctatact actgcttaag ttttacacct ataccagctt gaatatttta
    11281 tttttatttg cttaaatagt tcaactatac tggtttaaat aggttatctt tatttgctta
    11341 aatagttcaa ctatactggt ttaaataggt tatctttatt tgcttaaata gttcaactat
    11401 actggtttaa ataggttatc tttatttgct taaattagtt c
//
LOCUS       DAEMHW010000122        11362 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEMHW010000122 DAEMHW010000000
VERSION     DAEMHW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.122"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            243..1418
                     /locus_tag="KPK67_002616"
     CDS             243..1418
                     /locus_tag="KPK67_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2459562.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(1591..2988)
                     /gene="asnS"
                     /locus_tag="KPK67_002617"
     CDS             complement(1591..2988)
                     /gene="asnS"
                     /locus_tag="KPK67_002617"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBG2459563.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(3065..3298)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3467..3661)
                     /locus_tag="KPK67_002618"
     CDS             complement(3467..3661)
                     /locus_tag="KPK67_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBG2459564.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(3934..5607)
                     /locus_tag="KPK67_002619"
     CDS             complement(3934..5607)
                     /locus_tag="KPK67_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG2459565.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVALWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(5617..9015)
                     /locus_tag="KPK67_002620"
     CDS             complement(5617..9015)
                     /locus_tag="KPK67_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG2459566.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGEHILNINIWPDFVDDFSVHLINPSNNQTQAISLTSGEIRNTFGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSIATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNIQI
                     HNDLENEIYISKNASRQSGTLSGIDVVHTPEFEEELAGLGMNQNFFKISDSLGVLSIN
                     NTDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGALISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDGFYNS
                     AMLFAASQYAYKKAFELGRPLVINVSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEEEEELSLELWLNRPDKADVIIVSPTGEESKR
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(9183..10379)
                     /locus_tag="KPK67_002621"
     CDS             complement(9183..10379)
                     /locus_tag="KPK67_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG2459567.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(10400..11221)
                     /locus_tag="KPK67_002622"
                     /pseudo
     CDS             complement(10400..11221)
                     /locus_tag="KPK67_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
BASE COUNT         3625 a         2062 c         1312 g         4363 t
ORIGIN      
        1 atgcttaatt taaatttatt ctattcttta ttttcagtag taaactatct gtaaatctta
       61 attataataa agtattaatc aataaagttg ctcgacatta tctaataaat tttatctatc
      121 acaattttag attgttacac acaatatatt ttgatataat taaatcataa acgaagtatt
      181 ccaaatatct aatgatagaa aggaatactt atttttatgc tttattttta atggaggctt
      241 atatgaactt agaaaaaata aaaaaatttt ttggatttcc tctagacaaa aaacacaaag
      301 acaatgttga gaaagaactc tgcaattatc tttatcatac gggtaaaatc atttgcataa
      361 taatagtaat atctcaaata attatgatga tatcaatctc agctagagaa ggaggccctt
      421 ttgtcacacc aagacgccaa tggtattttt atatgtatgt aacactattt tgtgtatctt
      481 ctgtctttat gtttgtattt tcttaccttt ggaataaaaa aaaggattgc tgtacgtttt
      541 atataaattt atggataatt tatgtgactt ttttctgcct atggggttgt gtaattactc
      601 tacttgacca gttgggtgga aatgacctaa gtgtatttag ttatattatg ttagcagctg
      661 cagctttctc agtattaaaa tttggagaat cagtaatagt ttttggagga tgctttttgt
      721 ttcttaattt attcttacct gggattcaaa ctataccagg caatgagttt agtaatattg
      781 tcaacacatt ttttataaca tcccttgcca tttttgtttc tacaatgcta tatagaaatc
      841 gtgtatttac ttactatgat aaacttttaa ttagtcaaca atatgaagaa atatctcata
      901 taaataattt acttaacgac aaagttatga cagatgacct tactaaaatg aacaaccgta
      961 gataccttga agatattcta caaaaaaaat taatcagtca tgcaaatcaa gaacctataa
     1021 ctggaatgat gatagatata gattatttta aacaatttaa tgataattat ggacatcaaa
     1081 agggagacta ttgtctacaa aatattgcca aagtaatttt agattttaca agttctgaaa
     1141 atgaacatgc tatacgatat ggtggtgagg aattttttat atgtattttt aactgtaatg
     1201 aggcagatgc taaacttaaa tcagaatgtt tacgaaaaat tatagagtcc tgcaattttg
     1261 agcgtgatga tgttccttta aaatgtgtaa ctgttagtat tggagtttat acaaagaaaa
     1321 ttggaaagac aataacatta aatgagttta taagtaattg tgataaggct ctatatactg
     1381 caaaagaaca aggacgtaat tgtgttgcat tttattaaaa ggttacatac tattacataa
     1441 acctataagt attatatcgc aaaataaaag taatcaaatt atatatttat agtacaataa
     1501 aaataacttg catatctcta aaatagataa atatgcaagt cattttagtg tttatactta
     1561 aagttatata agttttatta tatataatta ttaaaattca gcatttttag gagttcttgg
     1621 gaaaggtata acgtctctta tgttagacat acctgtcata tacataatta ttctttcaaa
     1681 tccaagacca aatccagagt gagtagcagt accaaacttt ctaagttcta aataccacca
     1741 ataatcttct tctcttaatc ccatttcatt aattctatct aacaatacat cttctctttc
     1801 ttctctttga gaacctccta cgatttctcc aactccagga actagtaaat ccattgctct
     1861 aacagtttta ccatcttcat tcattctcat atagaaagct tttatatctt ttggataatc
     1921 tgtaacaaac actggacaat tatatatttg ctccgttata tatctttcat gctcagtttg
     1981 aaggtcacat ccccactcaa caggatactc aaattcatgc ccagattcaa ttaataattc
     2041 cactgcttta gtatacgtta ttctaataaa ctcagagttt actatcttat ttaacctttc
     2101 taacaatcct ttatctataa aactattaaa aaattccatt tcttcaggag cattttctaa
     2161 tacatagctt ataacatatt ttatcatatc ttctgcaagt tccatgttat cttcaaggtc
     2221 tgcaaaaact atttctggct ctatcatcca aaattctgaa gcatgtcttc ctgtataaga
     2281 gttttcagct ctaaatgtag gtccaaaagt ataaacgttt ctaaaagaaa gtgccattat
     2341 ctcggcattt aattgaccac taactgttaa attagcctct tttccaaaga aatcttgagt
     2401 atagtctatt ttgccatctt cagtttttgg aatatcattt aaatccatag ttgttattct
     2461 aaacatctct cctgcacctt cacagtcact acctgttatg attggagaat gtgcatatac
     2521 aaagtttctt tcttggaaga atttgtgtat tgcataggca gctattgatc ttactctaaa
     2581 tacagctgaa aaagtattac ttcttggtct taaatgtgct atcgttctta aatattcaag
     2641 cgtatgtctc ttcttttgta gtggatatga actatctgat tccccttcta caactatgct
     2701 ttttgcatgt atttcgactg gttgtttagc cccttctgta gatactaact ctccctctac
     2761 aagtatagat gagcttattg ctaattttcc tacctcttta aaattttcta ctttctcatc
     2821 aaatactatc tgcatatttt taaagaagct tccatcattt agctctataa atccaaaatt
     2881 tttagatact ctagatgttc ttatccatcc agctactttt acagtcttcc ctatgtactg
     2941 ttctttttct ctgtacaatt cttttactgt tataaattcc ttttgcattt taaatcctcc
     3001 tagtataatt gttattaatt tcttttaaat ataatttcaa taaaaaaagc ccttcatccc
     3061 tatataaagg gacgaaagac tattactctc gcggtaccac cctagttgtc ataatgacct
     3121 cttagttaag caatgcttaa agcctataac ggtgccttcc gtctaagtct acttactaaa
     3181 taagctttcg attagaaact ccaagatgtt cttcaatata aacctcgtat tgagctttca
     3241 ccatcctcaa ctcgctagaa ctacatttta tatctactct tctcttcaca gtatttatct
     3301 tttattattt ctgctaatta acaaaattat accaatttat aaatttttag tcaataaatt
     3361 tatataattg atactgattg ttatttaaaa tataataatt ctaccttaca taatgataat
     3421 aaatacttat cttcattact tctttattac ttcaatttcc aaaattttat aatttacttc
     3481 cattgtcaac ccagtctttt gcttcttcga cacccttaat tgaaggtatt gaaactttac
     3541 tatgaggttt taatttttca acatcagccc atgctgctgt cgactcattt ccaactaaaa
     3601 gtgaactttt aagtctttta ttatctttac cttttaatgc tcttgccttt ttactttcca
     3661 aaactatcac ctctattatt tattttgtaa tagttttagc aattttacaa ataaaattca
     3721 taagtctaaa ttataattat ttttaaatta acatcatatc ttttcaatac ttaatttata
     3781 cctatttata tacaaaatat ttactttgtg tattcaaaat ataaaactgt tttttaattt
     3841 tcatatttat tttttatgct atttttctac tctatctaaa gtatttgata caattaaact
     3901 aataatcaat tataatttta catagtttct tatctataga gtatttgcta tctgttgaat
     3961 cgtatttttt aaatttaaaa tcccatatcc ctgcgataca tttggatatg tatatatctc
     4021 aagttttgta gcacccaata ttaagtatgt ttttaaaact tgtgtaaaca atgataatct
     4081 cggtacatta tcctgttttt ctaagtattc catcaataag gctaaaacac ctgtgactat
     4141 agaactgctc actcctgttc ctgttcctgt attatatgtt ccatttttat atgtggatat
     4201 tatatctaca cctgaagcta ctatatctgg cttaattcct cttcctctaa ttggtccttt
     4261 cgaagaacca atccacatac tatcagtctt attgttaaat gtaccaactg taattacatc
     4321 atcactagca gcatacatag ttatagtagc tactgaatca gggtctaaaa accttgtgtc
     4381 tggagctatc aaatttttat ttggcaagta tatgtcatat tctccattta ttataagttc
     4441 tgaagtcaat cttaaaatcc atactccagg ttttatatct cttaatctta tctccaaatt
     4501 ttcttttcca gaggtaatat atggataaat aaatctcatt gcataagtag tattttctaa
     4561 gttaaatttt cctctatata tataaaagtc tggagaatac cttatatcat gactaacttc
     4621 acctgaagga gatattatct gtgctcccac tttatctgga ccattcgtat ttagtgttat
     4681 atcaagagca tagtcatctc catcttgtat tattacatct tgaacttctc ccacactgct
     4741 aaatctgcca gaataatgaa tatctgtatt cccttgattt cctgcaccac tcactactac
     4801 cacaccagca ctactcaata tattaaaagt atctagtatt gaagtagttg ctacagcact
     4861 tgacttaact ccaatagtca aatttattat cagtggttta ttttctgttc ttgctatatt
     4921 agtaacatac gttatagctg ctaaaaaatc tgaaacactg taatttatcc ttccagcata
     4981 ataagtatct gtatatgatt ttaatttaac tactattaag tctgattctg tagtaatacc
     5041 tctgtactgc gaatttatcc ttccatttcc aactaatata ccagaaacta aagttccagt
     5101 acctatatta tcttgactca aactcccatc atttctattt atggctatat ttaactcact
     5161 tcttgtaaat tcacttccaa aaataaaacc ttctggaggt ggatttgtat ttgcttcttg
     5221 gtcccataaa tataaaactt tactagttcc atcatcattt ataaaatcag gatgcaaata
     5281 atctatccca gaatctataa ctgctaataa aattcctctc cctgttatat cattatatgg
     5341 attttcataa atataatcag tttcagctgc tgttgtaatg gtttctccat tatttacatt
     5401 attagttatt tcaattagac tgctcattgg ttcagattct tcccacaaag caacttgtat
     5461 tatattattt aaaatagttt catcaaaatc taaaggtaca tatattactg caagctgact
     5521 atttaaaacc atatacctat ttattccatt ttcttgtagg gcagattcta tatcaccttg
     5581 ataaattata caataagatt tttccataaa tccctcctat cttaaaacat caaacattcc
     5641 tctaacatcc aaaagcccat atccagaatt tgtatttgga tatactgtgt ttgaatcttt
     5701 tctagcacca gcttgcataa aagtttttat tttttgcaca tatgcttggt ttggatatct
     5761 accgtcaaca aaagtatatt gaaaatacat tgcagcagca ccagctgcat gtgcacttgc
     5821 tgctgctgta ccagttatag ttgcatacgt attcccagga taagcagcta ttatattaac
     5881 acctggtgct actatatctg gttttaacct gtcttcaata gttggccctc ttgatgaact
     5941 ttgccataaa ctaccattaa tagtattgta ggctcctatg gttattaaat catcttgaat
     6001 cgcaggataa tttatagtgt aaaaagggtc tacctctcta aatctagttc cactttttaa
     6061 aagttctcta tttggaaggt ataagttata tcttcctgtt ataatataaa ctcctactaa
     6121 tcgaatcttc catacacctc tttttgcatt ctttaaagta acattagtaa attgctgtcc
     6181 agaaaaagtt gttgggtata tatacgttat agaatattca gtcccttcta aatcaaataa
     6241 acctgttact ttattataat ttgaaattcc aacacgttta ctttcttcac cagtgggaga
     6301 tactattatt acatcagctt tatctggtct atttaaccaa agttctaatg ataattcttc
     6361 ctcttcttca tttagttcta actcaacttc aacagaacct ccaacatatg gtattattcc
     6421 agatgtatgt gtctgtgtat tgccctcatt tcctgctcct gctgttatac ataaccctct
     6481 agtaaagaat gctttttcac tattgctcct attggttaaa cctgccaagc tactggtacc
     6541 taaagacaca tttataacta gtggtcttcc aagttcaaaa gcttttttat atgcatactg
     6601 agatgcagca aacaacattg cactattata aaaaccatct atcttgccga gttttattat
     6661 tattagctca gaatcttctg ctatacctgc atattcacta tttacatttc caagccctgc
     6721 acatatacca ctcaacatag ttccttgacc aacttcatcc tgagatagac tagggtcatt
     6781 ttctgcgata gctcgattta tatcttctcg tgtatattct gtcccaatat aaaaaccatc
     6841 aggtggagtt ccttcttttg tttggtccca taaatatact atttttgaag tgccatctgg
     6901 ataaataaaa tctgggtgta gatagtctat tcctgtatct gcaattgata ttagagctcc
     6961 tcttcctgtt atattaatat taggattatt tttaaagaaa tttacgccca tttcttctgt
     7021 tgcaactaca cctccaaaag tacctcgatt aatttctcca agtaatgtca tctttgtagt
     7081 cgatacagtt cttatgattg agggaagttg caaaactcgt tgtatgctac tatagtctgt
     7141 attatttata gatagtacac ctaatgaatc acttatttta aaaaaatttt gattcatacc
     7201 taacccagct agttcttctt caaattcggg agtatggact acatctattc ctgatagtgt
     7261 tcctgattgt ctacttgcat ttttacttat ataaatttca ttttctaagt cattatgtat
     7321 ttgtatatta taaccatcta taaacttata actattttcc tcatctttaa atgacttatt
     7381 aatacttcta tatccaatat cttctgtttc taaatcttga tttatattag atagagtata
     7441 caaatctata tctgataaat ttaaaaaacc aaaccctgat gaattatttg gataagattg
     7501 attacttaat ctccttgctc cctttaaaag taatgccctt aatttctgtg aatacaaaaa
     7561 taaatcattt ccatttacaa ttccccattc cataaataat gaacaaaccc ctgtaacatg
     7621 aggtgtagct atgctagttc cagttaatgc tccacttgta cctcctggta aaaaagatac
     7681 tatatcttca cctggagcta ataaatcagg tttaaaaaca cctaattgtg tatctccttc
     7741 accagaaaat atagatacta tatctgttct tgaattaaaa ctacctactg ttataactct
     7801 acttgctgtt ccaggaactg ttacagtaag ctcttgagta ggaatcagaa atctagtatt
     7861 tctatttagt ccttcagaag ttggtaaata tatatttaca ttaccagtta ctatatctat
     7921 tggttcaaat acaattttcc ataacccagg agttatctgt gtatttgaac ttaattgtag
     7981 agtaactctt cttgtaagag aatacggtgc tatagggtaa aaataaccag ttattctagt
     8041 ttcaccgaaa gtatttctta tctcacctga ggttagagag attgcttgtg tttgattatt
     8101 tgatgggttt atcaaatgta cactaaaatc atcaacaaaa tctggccata tatttatgtt
     8161 taatatatgc tctccttccc caactataaa ctcaacttct tctgtaatat tattttgaag
     8221 cctaatcctt ttatgacctc ctttatctgc attatttcca gctgctacaa ctatattgtt
     8281 tttccaaaat aaacacatat catctatata ttgttcaaat aaagatgttc ctctatgtga
     8341 accttcatta cttccataac ttatatttaa tgtaacaggc attcttaatt ctaatgccct
     8401 atctaaaata aatttaattg ccctcataaa ctctgtactt cttgaaaaaa tatcggtctg
     8461 tatatttcca actctaacca ctattattct ggcatcactt gcaatagttg cacaaatacc
     8521 tgcaacatgt gtcccatgta agctagttgt tgaaataggt atatacatac ttccatctat
     8581 tgcattattt atatcttcat tagtatataa tgttccttct ctaaaacctt ctggtggatt
     8641 tccttgaata gattggtccc aataatataa tatcttagac cttccatcac tatctctaaa
     8701 cacaggtaga gtataatcta ttcccgaatc aattatacca attatagttc cttttccagt
     8761 taaaccagtt ctatttttaa atcctgttat tcctgtactt gaaaaacttt gcacatcctg
     8821 agtctgtaat ataaaaggct tttctataaa ttcaatttct ggataagtta aaagtatatt
     8881 tacatcttct tcattacttg atgttattat tgcatatgat gaattaagta tctctactga
     8941 tacacctaat tcttcttcta acctcaatat atctccattg tattttacaa ttaattcata
     9001 atttattata atcacctcga cttctaaaat tattattaat gtatatgttt tgtgtgagtc
     9061 taaaatgtat tatactatat aattttttaa gtttcagtta tacaatttat aatagatata
     9121 ttaaaaaaaa taaataatga tggtacaatt ttgaataaat cataccatca tttatattta
     9181 ctttatcttg aaaccattaa atttttcaat gctttctcaa gaccttttac agtcagtgga
     9241 aacatattaa attcactttg tatcattctt tgagtttgat accaataatt aattgaatcc
     9301 catccatctc taagttctgg attcatccat acaattttac tgtactttct cttaaacctt
     9361 ttaagccact ctataccagg tgtgtaatta tatggaccac gatagttacc accaatatga
     9421 agcaactcag aaggcgacat agaagcatct ccaacaatta taactttata gtttttatct
     9481 atatttttta atatccattc agtattaata gatttactta accaacattc aggtgttgta
     9541 aataatctat cataaataca gttatggaaa taatatatct ttacatcttt aaaatgattt
     9601 gatttactta cagactgaaa taatgtatta cataaagtgc tataccctct cattgaacca
     9661 cctgaatcca taagcaataa aagcttcact gtattttttc ttggcttctc aaaaaataac
     9721 tttaaatatc ctgcattatt acaagtctct tcaatagtct tatccaaatc aagctctgtc
     9781 tttggagcat ctattctagt agaaaattga cgaagtctct ttagtgcaac ttgaaattga
     9841 cgcatattta aaacttcatc atctctaaaa tctctgtatt ttctttctcc tgcaacttca
     9901 agaacagctc taccataagt agaatttcca cctatacgta taccagtttg cccttttcct
     9961 gaatgtccta actctgaagt gcctcctgtg cctatccaat aattaccacc attatgagga
    10021 gaatcttgtt cttttattcg ttcctccaat ttagctctta atttatcaag atttatattt
    10081 ggattttgtt ctaatctttc aaactcttct aaatctatat ctggtttttt aagccaattc
    10141 ataatctctt ctggaatttc ttcttggtgt tctatacctt taaaatactc caaaaacact
    10201 gcatcaaact tatcaaaatc actttcactt tttattaaaa tcatcctgca aagatagtaa
    10261 aaatttgaaa agtttgaata gcacagtcct ttatccaatg cttcaattaa agtcatccat
    10321 tcatttaaag atacatttaa tcctcttgac ctcaataaat aaaaaaattc aataaacata
    10381 attatccagc tcctattaac tataagtgtc tttgcataga ttcaatatcc tcattattct
    10441 taagtagtat tcctgcaaaa ggaacttctt tttctatttt ttcaataggc attccactca
    10501 aagttaatgc ttgtatccag tctattaact cttatgtact aggctttttt tgtatgtcct
    10561 ttaaacttct aatccaataa aaagtagtca ttacttgttc caataggtgt tcttctactt
    10621 tatcaaaatg tactttaact atttcttcca tcatgtctct atcaggaaat tctatataat
    10681 gaaatataca ccttcttaaa aaagcatctg gaagttcttt ttccgcattt gaagttataa
    10741 taactattgg tctttgtttg gctcttacag tttcttttgt ctcatttatg taaaactcca
    10801 ttttatctaa ttcccaaagc aagtcatttg gaaattctaa atccgcctta tctatttcat
    10861 ctattaaaag aattacttgt tgatttgagc taaaggcctc tcctagttta ccatatttta
    10921 tgtacttgct aatatcatct acaccttctc ctccaaattg gctatcataa agtctttgaa
    10981 cagtatcata tacataaagc ccttcttgtg cttttgtagt tgattttata ttccaaatta
    11041 ctaaatcttt ttttaattca tttgaaattg cttgtgctag catagtttta cctgttccag
    11101 gttcgccctt aatcaaaagt ggcttttcaa gagcaatagc aacattaact gatgccatca
    11161 attctggtga tactacataa tcactacttc ctctaaagtt agatatctct ttttttatca
    11221 taaatcctac tccttttaaa cagtattttc taaatgtata tagactttat tactattttt
    11281 gatataaaaa taataataca atacatttgt atagtattat tctaaaattc tatatctaaa
    11341 taaaatcacc tctaaaatat tt
//
LOCUS       DAEMHW010000123        11320 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEMHW010000123 DAEMHW010000000
VERSION     DAEMHW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11320
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.123"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            144..932
                     /locus_tag="KPK67_002623"
     CDS             144..932
                     /locus_tag="KPK67_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG2459568.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(1055..1525)
                     /locus_tag="KPK67_002624"
     CDS             complement(1055..1525)
                     /locus_tag="KPK67_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBG2459569.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKALENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            1664..2308
                     /gene="uppS"
                     /locus_tag="KPK67_002625"
     CDS             1664..2308
                     /gene="uppS"
                     /locus_tag="KPK67_002625"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBG2459570.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            2351..3256
                     /locus_tag="KPK67_002626"
     CDS             2351..3256
                     /locus_tag="KPK67_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2459571.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            3571..5118
                     /locus_tag="KPK67_002627"
     CDS             3571..5118
                     /locus_tag="KPK67_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2459572.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMSYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            5121..5519
                     /locus_tag="KPK67_002628"
     CDS             5121..5519
                     /locus_tag="KPK67_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG2459573.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            5559..7292
                     /locus_tag="KPK67_002629"
     CDS             5559..7292
                     /locus_tag="KPK67_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG2459574.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            7524..8735
                     /locus_tag="KPK67_002630"
     CDS             7524..8735
                     /locus_tag="KPK67_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG2459575.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            8928..9188
                     /locus_tag="KPK67_002631"
     CDS             8928..9188
                     /locus_tag="KPK67_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBG2459576.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            9542..11254
                     /gene="ptsP"
                     /locus_tag="KPK67_002632"
     CDS             9542..11254
                     /gene="ptsP"
                     /locus_tag="KPK67_002632"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBG2459577.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT         4666 a         1040 c         1958 g         3656 t
ORIGIN      
        1 attttcctga atgtagttat agcatcttat gaaaggtgca aattgtaatt taaaattttt
       61 agtgtataat aagaattgaa aattcaaacc gataaaaatt aataaaaaat aattataaat
      121 taataatttg ggggaggaaa tgtatgataa aattaatagc aacagattta gatggaactt
      181 tgttagatga aaagagcgaa ataaatccag agttttataa agtatttaaa aaattaagag
      241 agagagggat aatgttttct gctgctagtg gcaggcagta tcaaaatctt ataaaaaaat
      301 ttgaagatat aaaagatgat atgatgttta tatcagaaaa tggaacacta gtagtatata
      361 aaggaaaaga aatattatca aatcctttaa ataaagaatt agtaaatgaa atcatagaga
      421 caacaagaag tataaaaggg aaaaaaattg taatgagtgg gaaaaaatac gcttatatag
      481 aaagcaaaga tgaagcgttt atacaagagg ttagtactta ttatgcaaag tttaaaattg
      541 tagaagattt gacgaaagta gagggagaca tattgaagat agctgtgttt gacttcaaag
      601 gtgcagaaca taataataat atctactttg aaaagttttc tgatagagct caagtatgta
      661 tttctggagt ggagtggctt gatttaactg caaaaggtgc aaataaaggg agtgccataa
      721 aaaaagtaca aaaaatgtta gatataaaat atgaagaaac tatggtattt ggtgaccagt
      781 taaatgatgt ggaaatgatg aaaagtgcct atcatagtta tgccatggaa aatgcaaatg
      841 aacatttgaa acaaattgct agatttagag caaaaagaaa tactgaaaat ggcgttgtag
      901 ataagataaa agaggttata aaaattggat agtcatatgt atagatgaaa aagattattt
      961 tgaagaaatc aaaaaaatat caatgtaatt ttgataaata agtttattta tgcgaaaatt
     1021 acattgatat ttaataaaca tcaataatag atatttattt ttttataata ttcagtattt
     1081 tgttttctat ttgagttgca ttttcagcag aaattaaata aacatatcta cctgccttct
     1141 caattatagt attatttgct atttcagaat tgattttttc tccatgtcct tctccaaaag
     1201 gtgcttgtat catatctttt ttataatttt ctagagcttt atagatttta tctacctcgt
     1261 cagtttttat aacgataata tcttctagag ataatttttc agaagaatta attacaaaac
     1321 cttgaattat gtcttcctct acatcattaa aatagttaaa atcctttgca tcaaagattt
     1381 ttgattcctt tataagtaaa tcagaagatt ttaatgcttt ttttattttt gatgtagaaa
     1441 cgtttttgta atcaccactt gaagaacaac taaccattcc taatgataag attatcgaaa
     1501 aaaacaatat taaaattttt ttcatgataa acctccaagt tttttaaact ctgtcttaaa
     1561 ttagtttact acataattat ataaaaataa atgtatttaa tgttacagtt ttgattaata
     1621 atgtaatatt ttgataataa tacctattga gaggtgatta gaaatgagaa taccaaaaca
     1681 tgtaggaatt ataccggatg gaaatagaag gtgggcaagt caaaatggta tgacaaaaga
     1741 taaaggatat gaaagtggaa tagaccctgg actagagtct tatagattat gtagagattt
     1801 aggctgtgaa gaggtgactt tttatggatt tacaactgat aatacaagac gtccatcagt
     1861 gcagacaaaa gcttttacaa aagcatgtat tgatgctgca aatatattaa tagatgaagg
     1921 tgctgcttta ttagttgtag gaaatacaaa atctccaatg tttccagaag aattattacc
     1981 atatactaat agaattaaag ccaaagaagg tcaaataaaa gcaaatcttc tggtaaatta
     2041 tggttggtat tgggacttaa ataatatatc aggagtagaa aatgtaaata aaaataatat
     2101 acaggattac atacagtcat ttgatgtatc cagactagac ttgataatca gatggggtgg
     2161 aagaaggaga ttaagtggtt tccttcctgt acaatcaatt tatgcagatt tttatgttat
     2221 agataattat tggccagatt ttaagagtga cgatataaag gacgcattta gttggtatga
     2281 tacacaagat gtcactttag gtggataatt aaaaatattc tgatttaagg ttgtagagtg
     2341 ttttaaatta ttgaaaaaaa aacttcttga tggtaaaata acactaataa tatgtaaaag
     2401 tgtaaaaatt tataccaaga agggtgaaga aatgtcaaaa ggcaataata acaataattc
     2461 aagaaacaaa tctaaaaaga ccagtcatct gaatagaaaa aagcgaaaat taaataagaa
     2521 aaagttagca gtgttgattt gctttacagt tttattttta tttattgcat ttaaggctac
     2581 tcaaggtgta gtagctttgg taaaaagtat ggataaaagt aataaaacta gtcaacaaca
     2641 aaatgttaac tcagaacaat ttgaccttgg taatgaagag gaaaataaaa agaaaaaata
     2701 tactgtattt atagacccag gtcatggagg caatgataaa ggaactgaaa gcaagaccag
     2761 taatagatat gaaaaagacc taaatctaca aattgcaaaa aaattagcaa ataaattatc
     2821 taaacagaag gatattcaag tagtagtatc cagaactgat gatacatata tatcattaaa
     2881 ggatagagct atcttagcaa ataatagttc tgctgatgtg cttgtttcta ttcatttaaa
     2941 tgcagagaaa aatggtaata cagcaacagg tatagaaact tggtatagga ataaagctac
     3001 agatggttca aaagaacttg cacagacagt acaaagtact atagtatcct atgttaaagt
     3061 tagagatagg ggaatagttg aaaataactt tgaagtactt agagaatcaa atatgcctgc
     3121 tatattaata gagtgtggtt ttttaacaac accttcagag gaacaaaaaa taataaatga
     3181 aaaatatcaa gaccaacttg cggaaggaat tgttcaaggt gtactttcat acctagattc
     3241 taaagggaat aagtaagtta actttacttt aatgtgagga taaatttaaa agtttaactg
     3301 tgtaaattgt attaacttta tttcgtaatg ttggaaatat tgtttgaatt tagaaactat
     3361 gaaattagaa attattttat ctatatctaa tttcatagtt ttttttgcaa aaaatttata
     3421 gttataaaat tttaaaaaat aaatttagta taatgtaatg tagttattta tatgtgattt
     3481 taacttgcat tgatttgcta tcaattgtgc tttataatta gtatatataa agtttaactg
     3541 taagataatt taaaaaatag gggaatttat atgaataaaa atagtatgac aagaatttac
     3601 agtttaaata aaataatttt tattataatt ggaattttag gaatagtttt actcacaaga
     3661 catattgagg tagatattaa agttttaatt ttaattttat taaccttgtt tagtataaat
     3721 gtatttattt catatcttaa aattaagttg tatgaggata aaattaattt aggattgata
     3781 aataattcag ataagtttat aaatattact aaagatgaga acttcctaaa acatgttcaa
     3841 aattatatta tcagtaatga aaaagaaaac tgtgtaatgg cttgttttga tttatgtaga
     3901 ctcaaactta taaatgatat atatggttat gagctagggg atgaagtgtt aaataatatt
     3961 ttaagtaact taaaaaatta ttttggaaaa gaagctattt atggaaagtt aaaaagtgat
     4021 gtttttgtat taataataaa gcttgaaaat agggaacaga agattcctta tttagtaaat
     4081 cttattaaga ataaaattgt gatactaagt cagattgaca gttttaaaat ggatataaat
     4141 attgaggctt ctattggaat ttataaattt aacaataatg aaattgatat aaaaaaagct
     4201 atagataatg ctgatatggc aagacttaaa tcaaaaggac taaagcacat agagtatgta
     4261 gaatttgata atgctatgga agaagaaata caatcaatta tgaaaataga gagagattta
     4321 ttcttggcta ttaaaaataa agagtttgta attcactatc aacctaaagt tgattcaagt
     4381 acaggtaaca ttataggttc agaggcattg ataagatggt tgcacccatc attaggcatg
     4441 gttggaccaa ataagtttat acctatagct gaaaaaaatg gtctgataaa caatattggt
     4501 agatggctaa ttcaagaagt ttttattacc attaacaagt ggatttcaga aggtataaat
     4561 gtcttaccag tctcaataaa tctttctaga gtagaactat ataaaaatga tttagttgat
     4621 ttttttaaac tgatgtttaa tacatataat attccaaaag aacttataga attagaaatt
     4681 acagaaacga cagcgctaag agatgtaaaa tttataagtg aaaggcttta tgagattaaa
     4741 tctttaggta tgaatatatc attagatgat tttggagtag gtaattcaaa ttttatcaat
     4801 cttaagggga taccactaga tattataaaa attgataggt cacttatatt ggatatagtt
     4861 acaaatacaa aaacaaagtt tatggtgaaa gcaattgtta acctttcaca tgatttaaac
     4921 gtaactgtta tatgtgaagg ggtagaggat atgcatcagg tcaaagtctt aagtgaactg
     4981 ggatgtaatt tcatacaagg ttatgtattt tttaaacctt tggatgagat gagttataaa
     5041 aaacttctta ctgacggaag ttatataaat ctgaaagata ctcttttaga taaatgcatg
     5101 acagttgagg aggagtaagg atgatttcag aagatgtaga ttattcaata agaatcataa
     5161 gagctatatt taataatgga gaagataaac caatagatgc taaaatgata ttaaaaagtg
     5221 aagatatacc aatactaaca ctatacagac tgcttagaaa gctttgtgca aacaatattc
     5281 ttacttttaa caaagaagat ggcaagacat acatgttaaa tgtgtcattg gatacaataa
     5341 gtctaagaac tattgtagaa gtaattgatg gaaaaatata tataaataaa tgtacgagat
     5401 atgtatctaa ttgcagtaat tgcttaaaag gttgtaaagt aaatcgtgaa ttgctagaaa
     5461 tagaaaaaga aatttataaa cttttatcga gaaaatctat aagacaaata ctagaataaa
     5521 aaattttcag ctatgataat aattggaggt tttcggttat ggatgatata tctcaagata
     5581 attttctact gtctaaagag tatgagaata gcttagatgt agatactaaa aaagcaagtg
     5641 gaatatatta tactcctaaa ataattgtag attatattgt aaaaaaaact ttaaaaaatc
     5701 acgatataat taaaaatcca tatccacgaa tacttgatat ttcctgtggg tgtggaaatt
     5761 ttcttttaga agtatatgat attttatatg acttatttga ggaaaatata tatgaattga
     5821 aaaagaagta tgatgaaaat tattggactg ttgataacat acaccgtcat atattaaact
     5881 attgtatata tggtgctgat attgatgaaa aagctataag tatattaaaa gatagcttaa
     5941 caaataaaaa ggtagtaaat gatttagatg aatctgatat aaagattaat ctattttgtt
     6001 gtgatagtct taaaaagaaa tggcgatata agtttgatta catagtgggt aatccaccct
     6061 atattggtca taaaaaactt gaaaagaaat ataaaaaatt tctcctagaa aaatatagtg
     6121 aagtttataa ggataaagct gacttatatt tttgttttta taaaaagatt atagatattt
     6181 taaagcaagg agggattggc agtgttataa ctccaagata ttttttagaa agtctatcag
     6241 gtaaagattt aagagaatat attaaatcaa atgtcaatgt tcaagaaata gtggattttt
     6301 taggtgcaaa tatatttaaa aatatagggg tatctagttg tattctaact tttgataaga
     6361 aaaaaacaaa agagacatat atagatgtat ttaaaatcaa aaatgaggat atatgtataa
     6421 ataaatttga aacattggaa gaacttctaa agagtagcaa atttgaacat tttaatataa
     6481 atcagagatt gctatctgac gaatggatac ttgtaaataa agatgatgaa actttttata
     6541 ataagataca agaaaaatgt aaatatagct tggaagatat agcaataagt tttcaaggaa
     6601 taattacagg gtgtgataaa gcgtttattt taagtaaaga tgatgtaaag ttgaaccttg
     6661 tagatgataa atttttaaaa tgttggataa aaagtaagaa tataaataaa tatatagtag
     6721 ataaaagtga atataggcta atatactcaa atgatataga taatgagaat acaaataaaa
     6781 ggatactaga tgaaattata gggctatata aaacaaaatt agaaaacaga agagaatgta
     6841 aaagtggaat aaggaaatgg tatgaacttc aatggggtag agaaaagctt ttctttgaaa
     6901 gaaaaaagat aatgtatcct tataaatcaa atgaaaatag atttgctata gattatgata
     6961 acaatttttc aagtgcagac gtatattctt tttttataaa agaagagtat ttagataaat
     7021 tttcatatga atatctagtt ggaattttaa attccagtgt ttatgataaa tactttaaaa
     7081 taactgctaa aaaaatgagt aaaaatatat atgattacta tcctaataag gttatgaaga
     7141 taaggatttt tagggataat aattatgaag agatagagaa tttatcaaag caaataatat
     7201 caattttatt aaataagtct atagataaag gaaaagtaga aaaattgcag ataaaaatgg
     7261 ataacttgat aatggattct ttaggtatat aagcagttgt aagtattaaa aaattataat
     7321 cttttaattg tttatttaaa tttagaaata aaaagttagc catataaaaa atattaaaaa
     7381 tatatacttt tcaatatttt tttgataaaa taagaatgaa tttgtgcaat tttatttttc
     7441 tccaataact aaaaatagtt ttttgaatta atatttgctt aagaataaaa atcagaaacg
     7501 tttttgatat agtaggtgat aatatgaaag gattagtttt agaaggtgga ggaacaaaag
     7561 gagcctacca aataggtgca tataaagctt taagagagct tggtatagag tttcaaggag
     7621 taactggcac atcaattggt gctttaaatg gagcatatat aattcaaaat gatattgaag
     7681 taatggaaga tatttggtta aattatgact atactcattt tatgaatatt gatagagaaa
     7741 cctatgagag gtttaaaaat gtagatttca ctactaaaaa tctcaatgca gtgtttgagc
     7801 ttataaataa agctagaaaa aatgaaggta tagatataag cccattaaga caactaatgg
     7861 agacaacatt aaatgaagac ataataagga aatcaaaaaa agactttgga cttgttacgg
     7921 tctggtggga taaaaaaatt aatccatatc cattgtatat cgaagatata cctaaaggaa
     7981 gattgattga ttatttaatt gctagttcca gtttgcctat ttttcaaacg aacatgatgg
     8041 atgataagtt gtatcttgat ggaatgtttt gtgataacat gccaatagga ctcttggaag
     8101 acaaaggtta tgaagatgtt gttgtaatta ggttgattga tgattttatt gggaaaatga
     8161 atctaaatcg acatcaaaat ataaatataa aaactataat accatcagaa tatctaggag
     8221 gttctcttaa taaagataga gataatgtag aaaaaaacat ccgactaggt tacttagata
     8281 ctatgaaagc atatgaaaga tgcactggaa ttaagtatta ttttgatgta gagtataaat
     8341 ataatgaaga atattgtttt gataaattta gaagtatgag caatgaaaca atacaatata
     8401 tctgtaattt attgaatata aaaagagatg cttcacttag aactgttatg gaaagtgtaa
     8461 taccaaagct tggtgaggtg cttggattgc caaaggattt ttcatataaa gatttgtttt
     8521 actgtatata tgaaaagaaa cttgaagaaa ataatataaa caggataaaa ttatatgatt
     8581 ttaacaaggt tatagatgtt gtaaatacaa atattgattc taatagaaat ttagcttcta
     8641 attttgaagt tagacctaaa attagtatta aaaagaacaa aaacacaaaa ttattgacaa
     8701 attgtattat aaaagacata aaaaatcaaa gttaaataaa aaacaaaaaa aaatttgtaa
     8761 ttatactcga aaaaagtgta tattagtagt ataatatttt tgtagcactg gtgggaacaa
     8821 aaaatgacca taggacaaaa aagtaccact tagtaccatt aaagtaaaaa acaggaaaac
     8881 aattttaaaa aatataaaat gtaaagttta taggaggatt taaaaaaatg gaaaaagtag
     8941 ttagtatcaa aaatgcaagt ggattacatg ctagaccagc aggaatgttt gttaagaagg
     9001 cagctgaatt taaatcaaca gtagaggttg tagcaaaaga caaaactgta aatgcaaaat
     9061 caataatggg aataatgagt ttaggtttag ctcaaggaga agaattaaag ttagttgtaa
     9121 atggagaaga tgaagaagta gctttagctg caatggttga attagttgaa agtggatttg
     9181 gagaatagat ataataattt tttaaaaatt tgatatttag aatctaatat tacttaaagt
     9241 aaagtaacag agaagtatta aacatataca gaccaacatt tgtagatatt taatttcttt
     9301 gttactttca tgtttattta atcagaagat taagactatt gttaattggg tataaaaata
     9361 taaatatttt agaaaatgaa ataagatatt tttaatcata aattcagagt aaaaatacag
     9421 taacaaatag tttataaaat tttataaaaa attataagta aataaaacta taaaaatgta
     9481 aattatttat ataaatttat aaagtttatg tgctagatga tattttttag gaggtagggt
     9541 tatggcttac aaaggaatag gtgcttctcc tggtgttgct ttagggaaag cgttagtagt
     9601 tgaacatagt gaattagtaa ttgaaaaaaa atctatagat aatgttgaag ctgaaattgc
     9661 taaattagaa gatgcagttg cagtttctaa agaagaatta gtaaaagtta aggaaaaggc
     9721 ttctgaagaa ttaggagcag aggaagcaga aatatttgaa gcacatttat tagttttaga
     9781 agacccagag ttaattggtt ctgcaataga taaaattaaa actgaaagtg taaatgcaga
     9841 gtatgcttta aatgaaataa aagagatgtt tgttagcatg tttgagtcta tggacaatga
     9901 gtacatgaaa gaaagagccg cagatataaa agatgttaca aatagaatac ttagacatat
     9961 attaggaatt aaagttgttg atttatctgc tttaagcgaa gaagttgtat taatagcaca
    10021 tgatttaact ccatcagaca cagcaactat gaataaaaaa atggttttag gatttttaac
    10081 tgatataggt ggaagaactt ctcatactgc tatcatgtct agaactttag aaatagctgc
    10141 aatcgttgga ttaaatgata taacttcaaa agttaaggat ggagattttg tggttttcaa
    10201 tggagacaca ggtgaagtaa tcgtgaatcc tgatgaagag actataaata aatatactga
    10261 attaaaagct aagtatgaag atgagagaaa agcattacag ctattaaaag gaaaaccatc
    10321 tgtaacttta gatggaaaac atgtggaatt agctggtaac ataggaacac ctaacgatat
    10381 agaaggtcta ataaagaatg atgctgaagg tgttggtctt tatagaacag aatttttata
    10441 catggataga gattctttcc caacagaaga aatacaatat gaagcataca aagctgtatt
    10501 agaaggaatg gatggaaagc ctatagttat aagaacattg gatataggtg gagataaaga
    10561 attaagttat ttaagtatgg aacctgaaat gaatccattc ttaggttaca gagcaataag
    10621 actttgccta gatagaaaag atatatttaa gactcaattg agagctttat atagagctag
    10681 tgttcatgga agattaagaa tcatgttccc aatgatttca tctttagaag agttgttaca
    10741 agctaaagaa gttgtaaaag aagttttagc agaacttgat tctgaaggtg tggcttatgc
    10801 taaagatgta gaaattggta tgatgataga agttccatct gctgctgtga tatctgatgt
    10861 attggctaaa cacgtagatt tcttctctat aggaactaat gatttaattc aatacacttg
    10921 tgcagttgat agaatgaatc aaaagataag ttatctttac aatcaattta atccagctgt
    10981 tttaagactt ataaagactg ttatagacaa tgcacacaaa gaaggaaaat gggcaggtat
    11041 gtgtggagaa tctgctggag accaaaaaat gataccaata cttttaggta tgggattaga
    11101 cgagttctct atgtctccaa tatcaatact tcctgctaga aagttaataa cttcagttaa
    11161 agagtcagat atgaaaaaat tagctgatga tgtacttaat atgggtactg ctgaagaaat
    11221 aaaaagctat atagaaaaga cttttaatat ataattaatg ttatatagtt actaaaattc
    11281 atttttctaa ataacaaaat tatattttac gaataaaaaa
//
LOCUS       DAEMHW010000124        11296 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEMHW010000124 DAEMHW010000000
VERSION     DAEMHW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.124"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..187
                     /locus_tag="KPK67_002633"
     CDS             <1..187
                     /locus_tag="KPK67_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459578.1"
                     /translation="AKCFNISQITQLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINT
                     GSIELGNERIYNYISKA"
     gene            complement(280..450)
                     /locus_tag="KPK67_002634"
     CDS             complement(280..450)
                     /locus_tag="KPK67_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459579.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(639..860)
                     /locus_tag="KPK67_002635"
     CDS             complement(639..860)
                     /locus_tag="KPK67_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG2459580.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            complement(944..2170)
                     /locus_tag="KPK67_002636"
     CDS             complement(944..2170)
                     /locus_tag="KPK67_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2459581.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            complement(2232..3164)
                     /locus_tag="KPK67_002637"
     CDS             complement(2232..3164)
                     /locus_tag="KPK67_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBG2459582.1"
                     /translation="MDIQLNILLFFKNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLNLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFIFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            complement(3253..3780)
                     /gene="hpt"
                     /locus_tag="KPK67_002638"
     CDS             complement(3253..3780)
                     /gene="hpt"
                     /locus_tag="KPK67_002638"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG2459583.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            4009..5085
                     /locus_tag="KPK67_002639"
     CDS             4009..5085
                     /locus_tag="KPK67_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG2459584.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            complement(5171..6379)
                     /locus_tag="KPK67_002640"
     CDS             complement(5171..6379)
                     /locus_tag="KPK67_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG2459585.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(6745..7128)
                     /locus_tag="KPK67_002641"
     CDS             complement(6745..7128)
                     /locus_tag="KPK67_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2459586.1"
                     /translation="MLNKKLPDAELKIMKYIWNTGYKTVISKDVADEIEKTYGWKHTT
                     TITLLARLTKKGFLISQRIGKHVHYTVLVKEREYLKLEGKRIFGGLHNNPLSELISKL
                     HDGEEITEEKIIEIGNWIKSWKDED"
     gene            complement(7154..7543)
                     /locus_tag="KPK67_002642"
     CDS             complement(7154..7543)
                     /locus_tag="KPK67_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2459587.1"
                     /translation="MEQQEVIVKLRRGELLLMEYMWRKKSILYKKEIISAMQERYKWR
                     KSTTEILLKRLVKMKVLKKKIVSFNYEVLVTKKEYLNAIREERNINIDDDFFTRVFTT
                     IHKRNQMVEEKFKKFITNIKDMIKRHV"
     regulatory      7764..7860
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            7915..8091
                     /locus_tag="KPK67_002643"
     CDS             7915..8091
                     /locus_tag="KPK67_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459588.1"
                     /translation="MFKDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            8238..8537
                     /locus_tag="KPK67_002644"
     CDS             8238..8537
                     /locus_tag="KPK67_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459589.1"
                     /translation="MDVEQSKNVYLRIGKELSEKMTKSELTKFSQEVANYLQYAGLDK
                     NMKIKLFQTIIEAVARKKVSLPSDSEFTEGILQSDEAEMTMNAGFIIEGLSYHNN"
     gene            complement(8782..9252)
                     /locus_tag="KPK67_002645"
     CDS             complement(8782..9252)
                     /locus_tag="KPK67_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459590.1"
                     /translation="MDKQKARRFLKVIDMNIDKIEEEAIKAFKESCLIEEANDIKIYI
                     NIQGKIEAIAVPTWAKLLENDKEINIFTLRQIETHLNDMFGELCYVNDYEEFENWCEN
                     ECENLNWDSYKKFNKENFEEIAERNIDDSSSVFLEKLHESIENCKQELQKIVEN"
     gene            complement(9283..9930)
                     /locus_tag="KPK67_002646"
     CDS             complement(9283..9930)
                     /locus_tag="KPK67_002646"
                     /inference="COORDINATES: protein motif:HMM:NF013043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459591.1"
                     /translation="MEIGDKFENLTILDIEQKNGRKYCLCKCSNCNNEKWIRADSLKR
                     IKACGCMKSNTQFKQNDLTGKKFGRLTAIKNTNKKAKSGHYIWICKCDCGNKIEVVEN
                     NLTTGRTKSCGCLKKESNIKNAKIALKVHKEKNIIDDTNLSIIKKTEAYSNSKTKIRG
                     VYYNKEKRKYCAQIEFKKTHYNLGYYDNIEDAEKAYIEAKDNLHNKFLEKLSEEE"
     gene            complement(9942..10391)
                     /locus_tag="KPK67_002647"
     CDS             complement(9942..10391)
                     /locus_tag="KPK67_002647"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459592.1"
                     /translation="MEKFIRLDFDKGFRGKEHRSSMCGDGEHFEAGISCYKISKEKCV
                     DAIINLCEYWFEFAGECQFKDFDINIFEGYYVGEGVSYEDLATCENHLHCIDGSLFNE
                     VYDLYYKHETYLEENGDIEELEENYKDEYITTEEFETKIKEMFIKYL"
     gene            10543..10740
                     /locus_tag="KPK67_002648"
     CDS             10543..10740
                     /locus_tag="KPK67_002648"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459593.1"
                     /translation="MKDVFEELISIKDACELYGKGESTLRLNIRNGKFIEGIDCKKFG
                     TTWVFKKTALEREYGKIGIKK"
     gene            complement(10753..11049)
                     /locus_tag="KPK67_002649"
     CDS             complement(10753..11049)
                     /locus_tag="KPK67_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459594.1"
                     /translation="MLIQEKSFYPNNIYPQIDFLKIKRQLKSIYKNDLSDCGSICIIE
                     KEHYSISVNSIGEVNIYYDSKFKQCAQDAVKDIEGMFKSQIRSFYLIDRLEGSN"
     gene            complement(11066..>11296)
                     /locus_tag="KPK67_002650"
     CDS             complement(11066..>11296)
                     /locus_tag="KPK67_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2459595.1"
                     /translation="ADILGLYYKREKESYLVTDIKDYKPHRTQNLYVIGGVTCNKMKE
                     MSGTTGEKFTQLYGNDVWSTMDKAIEFVKEKL"
BASE COUNT         3699 a         1833 c         1214 g         4550 t
ORIGIN      
        1 tgcaaaatgt ttcaatatct ctcaaataac ccaattaaaa aacttactaa gtaatactgg
       61 ttataattta ttaattccat ataaatactt aactgctaga gatatagaag gaatcgttac
      121 tcttattaat actgggtcta ttgaacttgg aaatgagaga atctacaatt atatatcaaa
      181 agcataataa tttaaaccta ttataaaaag gcaatttatt taataaaaaa tatttttaat
      241 attaaataaa ttgccttttt aattttggac taaatactgc tatccaagtg ctttttctag
      301 tttagataaa tgttgtattg gataatattt tagtagctct ttaaattgtt ctactgataa
      361 accttctgtt gttgtatata ttcttatttt ttcatctaag tcactattaa taaatttgtt
      421 ttttaaatcc tcaaaattta cactactcat tttacatcac tccttatgta acatattcat
      481 ttgtattatt ttatccaaat acataaaatt tattacataa aaatatatct atttaataat
      541 ttaataaaaa aaagctatct caatttagaa attactttct atatttgaga tagctctagc
      601 taaactaata gccaatagtt ataaataatt atttataact atctgttgct ttttccagcc
      661 atttgttgtt cagccatttc aactaatttt ttagtcatgt atccacctac ataaccattt
      721 tctcttgcag taaggtttcc tttatcaact tgttgataat tactcatacc aagttcatta
      781 gctatttcta atttcatttg gtttaaagcc tcttttgctc ctggtactac tgttctgttg
      841 ttgttattgt tacttgccat gttgattacc ttccttctgt ttaatttatt ttgtgttgct
      901 gataatatta tgtgatttta aacattttta attcaggtaa tttttagcaa ttaaaacctg
      961 ccaaatatcc agtagaaaaa gctatttgca aattgaatcc acctgtatat gcatctatat
     1021 ctattacttc tccagcaaaa aataaacctt ctactaattt tgattccatt gtgcttgaat
     1081 ttatttcgtt tacttttaca cctccagaag ttattatagc ttcttcaata ggtctgtatc
     1141 tctttactgt aaactttaaa ttttttaata aatggactaa gttttttctc tgctctctag
     1201 aaatttggtg aacaaccgta tttggattaa tttcacttaa gtttattatt ataggtatta
     1261 gtttttttgg aagtaaatca tctaatgctt tttcaaattt tttatttgta tacttctgaa
     1321 aatctttttg aactctttta tccagttttt cctcatctaa tgcaggtttt aaatctaata
     1381 atatagtgta attttctttc cttgtatctt tcattctaca acttgcagac ttgattatag
     1441 gtccatctaa accaaatcta gtaaattcaa gttctccaaa atcactataa actttctttt
     1501 gtttgctatt aaacaccctt atttcaacat ttctcaatga taatttttct aaatcttttg
     1561 taaaactttc ttgtacttct ataccaatta aagatggttt tgtatctata attgtgtgcc
     1621 cctgtgaaat tgcaaattta tatccatcac cagtagaacc agtaagtgga taacttaatc
     1681 caccagttgc tactacaact gaatcacact taatttcttt tttatcgttt aaaattactt
     1741 tctcaatttt attattttta gatattattt tttctacttt cgaatttaat aatatattta
     1801 ctttcttact tttaagttgt ctttcaagag catttacaat atcaaatgct ttatcactct
     1861 ctggaaaaac cctttttcct ctttctgttt tagtctttac tccaagatta ttaaacatac
     1921 ttattacatc atcatttgta aaagtataaa aagcactata aagaaatttt ccatttgtag
     1981 gtacattttc tattaattcc tctatttcac aagcgttagt aatattacat ctaccttttc
     2041 ctgttattgc aagttttttt cctaatttat gatttttctc aattaatgtt acatcatagc
     2101 ctttctcaca tgcagttgat gcggctatca tccctgctgg tccaccacct attattacta
     2161 ccttcttcaa ataatcactc ttttctaccc ttaatttttt taatttaaac tattgactct
     2221 ataccttaaa atcaatttaa ttttaaaagt ttaaataatg ctggagctcc tgctattcca
     2281 taaaaaacaa ctactgtatt cttaataaaa ttcataaaaa ttatactcat agtagagtca
     2341 cttatattta aagtgactat taacattgaa ataaacaact taatgccaat atataacatt
     2401 cctattgtta ctatcccaac caaaaaccta catattcttg aaaaaattat atttttactc
     2461 cccttatttt tattgctaaa atttattttt ttgttattat tatctgtatt aaatttaaca
     2521 aatctatcct caaccatata tccaaatata aatcctacta ttataccaaa tttttcaaaa
     2581 tattcagggc tatttagaaa tatagccact attataaatg gtataagtaa aactacaagt
     2641 aaaatataat ttttattatc atcaatatag tcaaatattt ttacaaatac aactgtaaaa
     2701 aatattccta atatccaacc cgctatcaca tctattggcc aatgaacagc taaatacagc
     2761 ctagacaatc ctactcctaa aaccataact gcacctatta aattaagata tctcttttta
     2821 aatatagtca taatactaac ccaaaatgtt gttgcagttt gagtatgagc acttggaaaa
     2881 gaataaccac cagcagtaga aactctcaat gattcaagac cttttattcc tattggtctg
     2941 ggagctttta caaactcttt gatgcctaaa tttacaacaa aatttccaat tatagcaaac
     3001 agtattttct gtccacattt tttatttata caccagtata taattgttgt aattaatatt
     3061 aataacggtg cttctgtgct tattgtaaag acaagaaata taaagttcaa aattggattt
     3121 cttaaatttt tgaaaaataa taatatattt aactgaatat ccatatagat gtctccttta
     3181 ctaaaatttt atataattaa ctaaaaagca ctaactccct tagaaattag tgcttttgat
     3241 tcacatttaa atctattcta catcctcaac tatacctata taaggtaaat ttctatactt
     3301 ttcagcatag tctattccat aaccaacaat aaatttgtct tctataacaa atccaacata
     3361 gtctacaggt atattagctt ttcttctctg aggtttgtca agtagagtac atagttttaa
     3421 agattttgga tttctagttt ttaaagctgc aactagatta cttaatgtta aaccagaatc
     3481 tattatatcc tctacaatca acacattctt tccttcaata tctacatcta aatcttttaa
     3541 tattttaact gttccagaag actctgttga gtttccataa cttgtaacac tcataaaatc
     3601 tatatttaca tccaaatcta tacatcttat taaatctgaa acaaatacac tagctccttt
     3661 aagtatacct accactagta aatcttcgcc tttaaagtct tcttctatct tttttcctaa
     3721 ttcataaact ttttctttta tttgttcttc tgtcaacatt tttcctgtaa ctttgtacat
     3781 tttgtatcct cccgctttcc ttctattaac ttatatatta tagcatacca aaatatcaat
     3841 taaaatcttt ttcatcattt ttaaacctaa attaacagct tatccatact tttttgcttt
     3901 ttccattaaa aatttggaat aacgattcct ttgtattata atctatatgt acatatatta
     3961 atcagttatt tttatcgatt atataatttt atttttggag gtacttttat gagcacaaac
     4021 catggagcta atttatatag tttatctagt aagtatggct tttcaaagga agaatttatg
     4081 gattttagct caaatataaa tccttttggt acatcaagtt tagccaaaca atacattgta
     4141 aacaatatag atatggtatc tatgtatcct gaccctgatt acatagactt aaaaacttct
     4201 atatccaatt actgcaaatg ttctatagat aatatagtac ttggcagtgg agctacagaa
     4261 ctaatttcat catttataca tacaattaat cctaaacagg ctcttttact ctctccagcc
     4321 tattcagagt atgaaaaaga gttatctaaa attaattgtt ctatagaaaa atactttgca
     4381 aaagaagaag ataattttca tataaattta gaaaatctta taaaaactat caatgctaaa
     4441 gattatgatt tggtagtaat ctgtaatcct agtaatccaa ctggatttgc atttactaaa
     4501 gtagaagtga gagaaatttt aaaaaataca gactcatttt taatgattga tgagacttat
     4561 gtagagttta ctgatacaga tacatactct tgtactcaat tagttgatga ttattctaat
     4621 ttattcgtta taagaggtac atcaaaattt ttttcaacgc ctggaataag gcttggatat
     4681 ggtcttatat ctaacactaa tgtcaaaaat gaaataaata aaaatctaga tttatggaat
     4741 ataaacataa tagcttctaa aatgggtgaa atcatgttta gtgatttaga ttttatatca
     4801 aatactattt ccttaatgaa cactgaaaga gattacctgc ttaaagagct taagaatata
     4861 aaaagcttag atatatacaa tactaaaggt aatttcatat tgtgtaaaat caagacaaag
     4921 gaattgactg ctaaatctct tcgagagcaa ctgctcccac aaaaaataat aataagagat
     4981 tgttgttcat ttgaaggtct agatgagtat ttctttagag tatgtatatt aaaacctaat
     5041 gaaaataaat tattaatatc atctcttaaa gcaattttta aataaaaaaa tcactttatt
     5101 ttataatttt ttataccata aaaaatgagc tatctctatt tgagataact catttttgat
     5161 tattaaaaaa ctaagcactt tgctcttctt tgtttccttt tttactcatt ccatcattta
     5221 ttttatctac tactactgct attgcattat ctcctgatac attacatgct gtaccaaaac
     5281 tgtcttgagt taagtataaa gctatcatta aacttgcaag cccaccatct gatggtattc
     5341 caaccattgg taggaaagga agtgcactca ttatagcccc acctggtgct cctggtgctg
     5401 caaccattgc tatacctaac attgcaatat aagggaataa tacacctatt ccatgtggca
     5461 taccattcat cattaaaact gcagttgtaa atcctgttat tgatatcata ctacctgata
     5521 aatgtattgt tgcacaaaga ggtacaacaa attctcttat ttgcttactt actccatttt
     5581 tttctgcaca accaacatta actggtatag ttgctgctga agattgagtt cctatagctg
     5641 ttaaatatcc aggtacttga ttttttaaca ttcttactgg attttttcct gaaaatacac
     5701 ctgctgctgt gaattgacat gccatatata caaaatgtaa aggtattact actaagtata
     5761 ctttccagaa tacccctaat attgtccata cttcccctgc tatagttata tttgcaaaag
     5821 ttcctgctat gtataaaggt aataaaggta taatcactgt tgacaacgtt ttcgttacga
     5881 ttccttgaaa ttcttggaag aaattaaata aagtctctcc ttttccatga tttcttaaag
     5941 ctgatattcc aagtcccatt acaaatgcaa atacgattgc agctgttaca tctaccatag
     6001 gctctaatgg aattgtgaat attggagcta acattcctgc ctctgggtct ccaatttgac
     6061 ttgcaacact tgcatcaata aaatttggga agattaatgt agatactgca aatgataata
     6121 atcctgctat taaggtagaa ccatatgcta ttcccgtagt tatccccaat aactttcctg
     6181 cacctgttgc taaatcagct atacctgctg ttacaaaacc tataattata aatggtataa
     6241 caaattttag gaatgctgag aataaagtac taactgttac taatatttgt actattattt
     6301 ctggtgcata acttccgatt aaaataccga ctatgatacc gatgattagt ttgggcacta
     6361 aacctatttt tttcttcata atatttaccc tccataactt ttttaaatta agcattcttg
     6421 cacattgaat acttagttaa atattggata tccacttatt atgataatag agttttacct
     6481 ctccgtcaac aatttaacca ttttttgtta ataaaaaaaa gaataacata tataaagtgt
     6541 tacttttatt tattttattt tttatatatg gctatttttc aatattttat aatgttgata
     6601 ctatttattc ttttaacttt cgtcagttaa atgtcagtaa aatattttat ccacaaattt
     6661 tacataagtt ttccacaact tatccccaat attatccaca agaaaaaaaa ggtagcactt
     6721 atattagtag ctacctttta tttattaatc ttcatctttc cagcttttta tccaatttcc
     6781 tatttctatt attttttctt cagttatttc ttctccatca tgcaattttg aaattagttc
     6841 tgacaaggga ttgttatgta aacctccaaa aattctttta ccttctaatt ttagatattc
     6901 tctttctttt accaatactg tatagtgtac atgtttccca attctctgcg atattaaaaa
     6961 accttttttg gttaatctag ctaaaagtgt aattgtagtt gtatgcttcc aaccatatgt
     7021 tttttctatt tcatctgcaa catcttttga tattacagtt ttataaccag tattccatat
     7081 gtatttcata atttttaatt ctgcatctgg taatttttta tttagcataa tatattcctt
     7141 ctttctataa aactcaaacg tgtcttttta tcatatcttt tatatttgtt atgaattttt
     7201 taaatttttc ttctaccatt tgatttcttt tatgtatagt tgtaaatact cgagtaaaaa
     7261 aatcatcatc tatatttata tttctttctt ctcttatagc gtttaaatat tcttttttag
     7321 ttactagcac ctcatagtta aaactcacta ttttcttttt taacactttc atctttacta
     7381 atctttttag gagaatttct gtagtacttt ttctccattt gtatctttct tgcattgccg
     7441 agattatttc ttttttatat agaatagact ttttcctcca catatattcc attaacaata
     7501 gttcccctct acgcaatttg actattacct cttgctgctc catttgtatg tcacttcctc
     7561 aaattcaatc tagcttaagt taataatagg agctttttta tacaaaaact acaggtaaaa
     7621 tttaccatat attcaaccca tttgttttgt tctataaggc gaaataattt tcatattatt
     7681 aagataactt ttaaaatttt taattttctt acaggcatgt tgaataataa ttaattctaa
     7741 tgtatcattg taattgtaga ataaaactaa atcagcaaaa ctagagaaat ttagtgacgc
     7801 aaagctatag ggactaagac ttatagagca tcttatgagt tatgtcagcc agttgccaaa
     7861 aagatagtgt tctttttgtt tttatgaaag ttttttaggg ggataataat atatatgttt
     7921 aaagataaaa tggataaatg cacacacatg ttgactgctt atattggtag ttcatatgac
     7981 tattgtgatt ttatagatac acaactagat gattttatac tagagtatgg agaaaatgta
     8041 gtagagtctt gcttacatca agtaatggta ttggtaagta agtataatta gatagacatt
     8101 gatttctaga gaaccctttt tagttttatc tacatttgaa gattattatg ttaaaaaatt
     8161 aattaaaata tttatatttg aagtattagc atttttaaaa tataatttaa ttataatcta
     8221 aaaaggggtt gaaaaaaatg gatgttgaac aatctaagaa tgtttatttg cgaattggaa
     8281 aagagttatc tgaaaaaatg actaaatcag aattaactaa attttcacaa gaagtagcaa
     8341 actatttgca atacgctgga cttgataaga atatgaaaat taaactattt caaacgatta
     8401 tagaagcagt tgcaagaaaa aaagtatcat taccaagcga cagcgaattt acagaaggaa
     8461 tattgcaaag cgacgaagca gaaatgacaa tgaatgcagg atttataatc gaaggattaa
     8521 gttatcataa taactaaatc tttagcatta aaaaagcagt gtatttattg aatacactgt
     8581 ttttatttcc gcatagttaa tctaaaataa tttggatatt atatcatata gatataaatt
     8641 aaattgatat ttaaatccct tatagttaat ataaaaattc ttattataat atcatttctc
     8701 aataaattaa aaaatatttt aaatccctta tagttaatat aaaaagcgat actatataca
     8761 ttaataataa tactcttcta cttaattttc aactattttt tgtaattctt gtttacaatt
     8821 ttcaatgctt tcgtgtaatt tttctaaaaa gactgatgag ctatcgtcta tattcctttc
     8881 agcgatttct tcaaaatttt ctttattgaa ttttttataa ctatcccagt tcaaattttc
     8941 gcactcattt tcgcaccaat tttcaaattc ttcgtagtca tttacgtagc aaagttcccc
     9001 gaacatgtcg tttaaatgtg tttctatttg tcttaatgtg aaaatattaa tttctttgtc
     9061 attctctaaa agtttagccc aagtcggaac tgctatcgct tcaatttttc cttgaatatt
     9121 aatataaatt tttatatcat tagcctcttc gattaaacaa ctttctttaa atgcttttat
     9181 agcttcttct tctattttat ctatattcat atctataacc tttaaaaatc ttctagcttt
     9241 ttgtttatcc attttaattt cctcccctat caaatatatt tatcattctt cttcagacaa
     9301 tttttctaaa aatttattat gtaaattatc tttagcttct atatatgcct tttcagcatc
     9361 ttctatgtta tcatagtaac ctaagttgta atgtgttttc ttaaattcta tctgtgcgca
     9421 atattttctt ttttctttat tataataaac tcctctaatt ttagtttttg aattagaata
     9481 cgcttctgtc ttttttataa tagatagatt tgtatcatca ataatatttt tttctttatg
     9541 tacttttaac gctatctttg cattttttat attagattcc tttttcaagc atccacatga
     9601 ttttgttctg cctgtagtta gattattttc tactacttcg attttatttc cgcaatcgca
     9661 cttacaaatc caaatataat gtccgctttt agctttttta ttagtgttct ttattgctgt
     9721 taatctgcca aactttttgc ctgttaaatc attttgttta aactgtgtgt tacttttcat
     9781 gcatccacat gcttttattc tttttaaact atctgccctt atccattttt cattattgca
     9841 attactacat ttacataagc aatatttcct gccatttttt tgttctatat ctagtattgt
     9901 aagattttca aatttatctc caatttccat tttttcactt cctataagta ttttataaac
     9961 atttctttta tcttagtttc aaattcttct gttgttatat attcatcttt gtaattttct
    10021 tctaattctt caatatctcc attttcttct aaatatgttt cgtgtttata atataagtca
    10081 taaacttcat tgaataaaga cccatctata caatgtaagt gattttcaca agttgctaaa
    10141 tcttcataac taaccccttc acctacatag tacccttcaa aaatatttat atcgaaatct
    10201 ttgaattgac attcacctgc aaattcaaac caatattcac ataaatttat tatagcatca
    10261 acacattttt ctttacttat tttataacaa ctaattcctg cttcaaaatg ttctccatct
    10321 ccgcacatgc tacttctatg ctcttttcct ctaaaacctt tatcaaaatc aagtctgatg
    10381 aatttttcca ttttttatac ccccctttgt ttatctcttt ctttaattat attataaacg
    10441 ttaccgttta ttaagtcaat acttttttaa acgttttcgt ttattttttt cttgtctttc
    10501 taaacttttg cgattataat ataaataaga ggagttggta aaatgaagga tgtatttgag
    10561 gagctcattt ctataaaaga tgcttgtgaa ctttatggaa aaggtgaaag tacgttaaga
    10621 cttaatataa gaaatgggaa atttatagaa ggtatagact gcaaaaagtt tggaacaact
    10681 tgggttttta agaaaacagc acttgaaaga gaatatggaa aaataggcat aaaaaaataa
    10741 ggtagcaact aactagttgc taccttctaa tctatctatt aaataaaaac ttctaatttg
    10801 gcttttaaac attccttcaa tatcttttac tgcatcttga gcacattgct taaactttga
    10861 atcatagtat atatttactt cgcctatgct gtttacagat atagaataat gttctttttc
    10921 tattatacat atgcttccac aatctgataa atcattctta tatattgatt ttaactgcct
    10981 ttttattttg agaaagtcta tttgggggta aatgttgttt ggataaaaac ttttctcttg
    11041 tattaacata ttctcacctt taactctaca atttttcttt tacaaattct atagctttat
    11101 ccattgttga ccacacatca ttaccatata gttgagtaaa tttttctcct gtagtcccac
    11161 tcatttcttt cattttattg caagttactc caccaattac atatagattt tgtgttctat
    11221 gtggtttata gtcttttata tctgttacta agtaactttc tttttctctc ttataatata
    11281 gtcctaaaat atctgc
//
LOCUS       DAEMHW010000125        11094 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEMHW010000125 DAEMHW010000000
VERSION     DAEMHW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11094
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.125"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..284)
                     /locus_tag="KPK67_002651"
     CDS             complement(<1..284)
                     /locus_tag="KPK67_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2459596.1"
                     /translation="MLNKKLPDAELKIMKYIWNTGYKTVISKDVADEIEKTYAWKHTT
                     TITLLARLTKKGFFISQRIGKHVHYRVLIKEKEYLKIEGKRIFGGLHNNP"
     regulatory      511..608
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   750..1178
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=750..778
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(1464..1622)
                     /locus_tag="KPK67_002652"
     CDS             complement(1464..1622)
                     /locus_tag="KPK67_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459597.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(1981..3351)
                     /locus_tag="KPK67_002653"
     CDS             complement(1981..3351)
                     /locus_tag="KPK67_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBG2459598.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            3565..4683
                     /locus_tag="KPK67_002654"
     CDS             3565..4683
                     /locus_tag="KPK67_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2459599.1"
                     /translation="MNIKSAFIRKRGEKFYVYVEYIEETTGKIKQKSYGSYEKKKDAE
                     KHLIEIKSTINNNKFVAPKDMTFVERCYKYIDMNKNNWSPYTVTNTKSWVKNYIEPFF
                     KNLKLTEVNPSSVQAFVNYNFNNSTFTSAKVRYSFLSSVIREAYRLREIQENSCDFIK
                     LPSKEKYSTNEIYNREEVLLLIEKLLGNNIEIPILLMLLLGLRMGEATGLRWSDVDLD
                     NNLINVNQILVYSNGKISFKEPKTPKSKRALSAPKELIEKLKIEKLRQSKLKLQGILE
                     NKNNLVCLNTNLNPFLPSSLRKTFHNFTKKNNLKKIRIHDLRHTSATLLLLGGTNMKV
                     VSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(5001..5669)
                     /locus_tag="KPK67_002655"
     CDS             complement(5001..5669)
                     /locus_tag="KPK67_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG2459600.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIEIIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(6524..8944)
                     /locus_tag="KPK67_002656"
     CDS             complement(6524..8944)
                     /locus_tag="KPK67_002656"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBG2459601.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(9298..9648)
                     /gene="rsfS"
                     /locus_tag="KPK67_002657"
     CDS             complement(9298..9648)
                     /gene="rsfS"
                     /locus_tag="KPK67_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBG2459602.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(9765..10337)
                     /gene="yqeK"
                     /locus_tag="KPK67_002658"
     CDS             complement(9765..10337)
                     /gene="yqeK"
                     /locus_tag="KPK67_002658"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBG2459603.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(10338..11027)
                     /locus_tag="KPK67_002659"
     CDS             complement(10338..11027)
                     /locus_tag="KPK67_002659"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBG2459604.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         3690 a         1890 c         1290 g         4224 t
ORIGIN      
        1 aatggattgt tatgtagacc tccaaaaatt ctttttccct ctattttgag atattctttc
       61 tcttttatta atactctata gtgtacatgt ttcccaattc tctgcgatat gaaaaaacct
      121 tttttagtta atctagctaa aagtgtaatt gtagttgtat gcttccaagc atatgttttt
      181 tctatttcat ctgcaacatc ttttgatatt acagttttat aaccagtatt ccatatgtat
      241 ttcataattt ttaattctgc atctggtaat tttttattca acataatata ttccttcttt
      301 caatttatgt cacttcctca aacttatcta gcttaaatta ataataggag ctttttatca
      361 caaaaactac aggtaaattt ttctatatat tctacgtatt tagtctgttt tttaagtgtg
      421 aaattacgtt catacctata aatttattca attttcaaac aatcttgttg aataataact
      481 aattctattg tattatttag ttatagaata aaactaaatc ggcaaaacta gagaaatcta
      541 gtgacgcaaa gctataggga ctaagactta tataaatatc ttatgagtta tgtcagccag
      601 ttgccaaaga gatattgttc tttttgtttt tatgaaagtt ttttaggggg ataataattt
      661 atatgtttag agataaaatg gataaatgta cacatatatt gactgcttat attagcagtt
      721 cgtatgatta ttgtaatttt ttggatacaa tttacattcc atatagttaa tataaaactt
      781 gtgtttattg tgatagtgcc gaacaggtgt taatacgatt tacattccat atagttaata
      841 taaaacaaga ttatagctga ttcactcaat gagatagctt ataatttaca ttccatatag
      901 ttaatataaa actgtttgta agcaacttat cacctaccaa tcaaataaat tatttacatt
      961 ccatatagtt aatataaaac aaaaaaaaag tgttcaaatt gctgtttgaa cacaaaaatt
     1021 tacattccat atagttaata taaaactatg attatgagaa taaaaaagat acatcaaagc
     1081 tagatttaca ttccatatag ttaatataaa acagcttttg aaattttatt ctggacaggt
     1141 cttagactaa tttacattcc atatagttaa tataaaaccc caaaataaat tgagtatttc
     1201 caatgcctac acatatacaa atctctcaaa tttgcagtga accatgagta gtgcaattga
     1261 taacacttat cacaagtcct caatgcctta tatttcaact gttaagcctt aatttcttca
     1321 aaaaatcgaa cactgcaaaa tttctatgac tttattatat cataaaaata ttatttttga
     1381 atatctgtac caatttgtga tataataaaa gcaagaagaa ctacaatcta ttttgctgta
     1441 gagtggagtt cataatttag aaattactta gttttatgga atctgatttt taaatcaaat
     1501 tcccaaccac tcttagttgc cgctttgagt ggttttttac gctttctaaa tagtttgcta
     1561 gctatataaa ctattaagct agcagataga cttgctagta tgccttgtaa aaaattatcc
     1621 atgtatattc acctcccttc ttaacgtcgg gaggataatt ttttgataca tgaactccac
     1681 tctataaatt gtagattaca tcttcttgct aaaaatatta taacatataa ttcttacata
     1741 ttttacctat ttattacttt ttacattata ataatattct tttaatcaaa aaaacaccta
     1801 ctattaaagt aaatgttttt aagtattttt aattttaaaa tccatatagt tcatatgaaa
     1861 ctaaatataa taactcaaca tatgagcaat atcgagacat ttacatacca catagttaat
     1921 ataaaacgaa aataaaaaaa ttaaacttat aaaaccctta ttaatttaca tacccactag
     1981 ttatttatat tcaaaaaaat cttctccatc tgggttataa aataatattc tacttgccaa
     2041 tccttgttca tatttataat tttctttatc acctacataa gaaaatgtta atgtcatgct
     2101 tcttgatgga tctaatgaaa ttatatctaa ataaatagta catttttctc ctggctttaa
     2161 tacaccgaaa ctattaaaat ccatttttat tttagcatat tgccccatat ataatgttga
     2221 attagcttct ccataaactt ttaattttgt tacatctaaa ttttgtgtac ttgtattttc
     2281 tatatttata ggaactgttg ggtctttata tttttcagta taatattcaa ggttatcatc
     2341 ataacattca agataacttc ctttatcttg ccaaattatg tgctttgaat caaaatcaat
     2401 ctttaatcca catattatat ttttatttga agacggaaga ctaatattat tgatggtatt
     2461 ttcacactct agcataattt tatcacttag atttcctatc ccagtcactt ttgcagcatt
     2521 tttaagtata tctttattac ttccaatatc aactagtaca ataggattat ttttagccat
     2581 tggtgaagca actaaagcat aaactaaatc agtaccacta gctatataaa attcttttgc
     2641 gccattatag aacttattta ctattttttt attagtatca tatctatcta ctccacctaa
     2701 tctagttgaa ttagtatcat taactagttt gtcactcatt gatgaagtac cacctattac
     2761 ataactttct acaccagttg tattaaatgg aacacttttt ccatcagtta atactatagc
     2821 tgctttatct ctaactgcta caggtgcagc actcattgca tcaggctctc ctttaaaagc
     2881 attagtcaat ataactttat ttactttgtt aattgagttt atttcttttg caacattata
     2941 gcttgtctta attctatcac taccttgaag tcttttagcc tctattcctt tatcttttag
     3001 aagagtttct gttgctttat caatagaact ttctccacct attatgtata cctttttagc
     3061 tttctctact cttttaagag ttgcattagg tatgttattt ttcttagtta aaaaaattgg
     3121 tgcatttgtt gctcctgcaa gtccactcgc acttaatcca tcagccattg tgctgtctgc
     3181 attaattaat atagctgtag tataattttg ttcatcagct ataagagcag ccgtttcata
     3241 tttatctttt ccttgaatag tataaattgt atcaagtgca ttagctgata taggaccagc
     3301 agcaaacatt gacacggcta accctagtga taataatttc tttaatttca caatatctcc
     3361 cccttttata tatgaaatac tatatatctt aataattcta cattaaattt tcttttcctc
     3421 ctttaacgat atttatcttc ctgttaattt aagtgtatac aaatacgtgt tataatgtaa
     3481 ctgtaaagaa ttaaaaagat aatgacataa ttagagcagt ccaactgttc ttttataaaa
     3541 ctaacttata aagggaatgt acatatgaat atcaaatcag cttttataag aaaaagaggg
     3601 gaaaaattct atgtatatgt ggagtacata gaggaaacaa ctggcaagat aaaacagaaa
     3661 agttatggaa gttatgaaaa gaaaaaggat gctgaaaaac atttaattga aataaaatct
     3721 actataaaca ataataagtt tgttgctcca aaagatatga catttgtaga aagatgttat
     3781 aaatacatag acatgaataa aaataattgg tctccatata ctgttacaaa tacaaaatct
     3841 tgggttaaaa attacataga accttttttt aagaatctaa aactcacaga agtaaatcct
     3901 agttcagtac aagcttttgt aaattataac tttaataatt ctacctttac tagtgctaaa
     3961 gtaaggtata gctttctaag ttctgtaata agggaagcat atagattaag agaaatacaa
     4021 gaaaattcat gtgattttat aaaacttcct tctaaagaaa aatattctac aaatgaaata
     4081 tacaatagag aagaagtatt attgctaata gaaaagttac ttggtaataa tatagaaata
     4141 cctattcttt taatgttact tctaggttta agaatgggag aagctactgg gcttaggtgg
     4201 tcagatgttg acttagataa caatctgata aatgttaatc aaattcttgt ctattctaat
     4261 ggaaaaatat cttttaaaga gccaaaaaca ccaaaatcaa aaagagcttt atcagctcca
     4321 aaagaattaa ttgaaaaatt gaaaatagaa aaactaagac aaagcaaatt aaaattgcaa
     4381 ggtatcctgg aaaacaaaaa taacttagtt tgtctaaaca ccaatttaaa tccgtttcta
     4441 ccttcttcac taagaaaaac gtttcataat tttacaaaaa aaaataatct aaaaaaaatt
     4501 agaatacatg atttaagaca tacaagtgct actctgctgc ttttgggagg tactaatatg
     4561 aaagttgttt ctgaaagatt gggtcataca gatataaaaa taactatgaa tagatattct
     4621 catgtactta aagaaatgga caaagaagct tccgacaatc taagcgaact tctatttaaa
     4681 tagtaaaaaa ctgactgtca gttaaatgtc agttaaacct aaaaaatagg ttttgtcagt
     4741 tgattgtcag tcaattattt tataatagtt tcacttaaaa acgtctatat ctacacattt
     4801 tactatttat ttatgcttat taatatctat ctgttattat taattctaat aacttctatt
     4861 taattattta gttcagttta caagaaaata cacacaaacc ataaaatgag atgttgacaa
     4921 cataacagac agtatgctcc tgtaaaaggt aacatactat ctgttatatt gtctggattc
     4981 ccaaattagc tatttggaac ttaactaaaa aatcctaata taagggcaac tattataatg
     5041 gcaatcggaa tacatactgt gtaatcctgt tccatagagt catcatctga atgtagtttt
     5101 acaattcgca atttagcaat cagtttgaag cagaaatagc ctattattgt tccaattaca
     5161 ttcgtgagta agtcgtctat atcggttgct ctgtgtaacg taaaaagctg aataatttcg
     5221 ataaaaagag ataatccaaa accaatcaag aatgtatttc tcaaacgttc aaaggttttg
     5281 cttataagcg gacataaaaa acctaatggg acaaaaagta aaatattcaa aataaaactc
     5341 aaactaaatc catcactaaa aggaataaga tttatgtttg gattaaagaa cgtttcccct
     5401 aactgtgaca gtcttatata ttcactgaat gtagggatac caacaatatg cgatagtgtt
     5461 aaacataagt agtaatacat tgccagtgat aaaatagatg tttttacctt tggaatatca
     5521 ctatttttct tgaatattac aactaagaaa ataccaatca agataattcc caacaagaaa
     5581 ataggattaa ttaacaataa atctttgata gcttcaaaca gactgagttg aatttctaca
     5641 cctgattgat tatttgttac agtctccatt tgtacatacc tcctcataac atttcaatta
     5701 tttctaaaaa acaaaataga agataaatct taaaatcatc ctatcaaaat ttttaaattt
     5761 gcctttatca gtttatacca ccagttaatt cctgtcttac ttctgtatgg tattcttggg
     5821 attctttcat caaattagac aaaaaaatgg ttaccatatc aataaactgg ttacgctcca
     5881 tatctcccga agcaaccatc ccctgagaat aaatccgtat caggaatctc cacatctact
     5941 gcacctatca caagttattt gataacatcc tcaaacaatc ccaacttgga tatatcagca
     6001 acacccatta gaatactact gctgaccatc cttgttttcc tggtgataat tttaatcaac
     6061 tgggaagttg aaacgcctat cttttttgca agttcctttt tagaaacatg gtagctatta
     6121 tatgaatctg aatttcgttg tctgggtgtt tcatataaag catagatacg cacctttttt
     6181 gcctgttttc attatataga aaaaaatttc aaaatacaat aattatattt ttttcgttta
     6241 agcttagagc agttccttga tgaacaacac tttggaaact gaatgattta cataccactt
     6301 aatacattaa ctcgatttaa cattaattat aataatattt aaaacaacaa ttagggtata
     6361 taagaatgaa ctttttagtt ctttaatcat ttttttgtat aaaatcaaat ctatttttat
     6421 aatttcaaca attttataat ttcaacaatg aaaaaaggct tatctctatt ttgagacagc
     6481 ctcatttatt ttataaattt atatttttgt tcatattttt gagttattta acaactatat
     6541 ttactaattt tttaggtaca gctacaactt taactatagt tttaccttca actaatcctt
     6601 ttattttttc atcttctaaa gctacctttt ccatctcatc tttagatata tttgagttaa
     6661 cagttaattt tcctctaact tttccattaa cttgtacaac aacttctatt tcatctttta
     6721 ctaaagcttt ttcgtcatat ttaggccagt ctatatcaaa tacacttcct tctttaccta
     6781 tcatagtcca tagttcttca cctaaatgag gggcaaatgg tgcaattata gttactatag
     6841 tttgtacacc ttcttttata actgcttcgt ttatattatc taactcttta tatttataca
     6901 tatcatttat taattccata agagctgata tagcagtatt aaatccgaat ttttcactta
     6961 aatcagcagt aactttttta agtgtagagt gtatagtata tctcatatcc ttatcttggg
     7021 aatttaattc tccaaactct acatcctttt taaccacatc tgctaactcg tcaaccaatc
     7081 tataaaccct atttaagaat ctaaagcatc catcaacacc ttgctctgac cagtctaaat
     7141 ctctttctgg tggtgctgca aataatacga aaagtcttgc agtatctgct ccatactcat
     7201 ctattatatc cataggagat actgtattcc ctttagactt actcatttta gaaccatcca
     7261 taagtaccat tccttgagta agtaagtttt taaatggctc attgaagtct accattccca
     7321 tactcttaaa tgcctttaca aaccatcttg cataaagtaa atgcattata gcatgttcta
     7381 ctccacctat atattggtct actggatgcc atctattaac taattcctta ctaaatggtt
     7441 cattttcatt attgctatcc acatatctta agaaatacca agaagaatca acgaatgtat
     7501 ccatagtatc aacttccctt ctagctggtt ttccacagtg aggacaagtt gttgacataa
     7561 attgctttga agtcgtaagt ggagattccc cttttccagt aaattcaaca tctgttggta
     7621 aaagaactgg taaatcttcc tttttaactg gaactattcc acattcatca caataaacca
     7681 caggtatagg acaaccccag tatctttgtc ttgaaactaa ccagtctcta agtctataat
     7741 taattgttct ctttcctcta ccttcttttt ctaacttatc gattattcct tcaaatcctt
     7801 ctgaagcatc taatccatta aattcttctg aatttatcat tttgttatct tctgttatta
     7861 catcaattat gtctaaatta tatttttcag caaaatctct atctctttca tcatgtgctg
     7921 gaactgccat tatagcacct gttccatact caactaatac atagttagct atccatagag
     7981 gaactttctt gccatttaaa ggatttataa catatctacc tataaacatt ccttcttttt
     8041 ctacatcagc tgctgttctt tctatttctg tcatagtatg cattttttga acaaaagctt
     8101 caacatctgc ttcatattca gtccctgcaa ccaattcttt aactaattca tgctctggag
     8161 caagtaccat ataagtaact ccataagttg tgtctggtct agttgtaaat actgtcatag
     8221 atttatcagt tccatctata tcaaaaacta aatctgctcc tgtactttta cctatccaat
     8281 ttttttgcat tgttttaacc ttttctggcc atccatctaa tgtatctaag tcattaagta
     8341 attcttctgc aaattcagta gtcttaaaat accattgttc taaatctttc tttaatactg
     8401 ttgctttaca tctctcacat gctccttgaa caacttgctc atttgcaaga acagtctcac
     8461 aagaaggaca ccagttaaca tatgattttt tcttatatgc aagcccatgc tctaaaaact
     8521 tcaaaaatat ttcttgtgta aatctatagt attctggagt tgaagttgca acttctttat
     8581 cccaatcata actaatacct aataaattta attgctcttt catttcttct atattttcca
     8641 ttgtccattt atgtggatgt attccatgtt ttatagctgc attttcagct ggtaatccaa
     8701 aagaatccca tcccattgga tgaagtacat tatatccttc catttttttg aatcttgcga
     8761 caacatcacc tatagaataa tttctaacat gtcccatgtg tatttttcca gatggataag
     8821 ggaacatttc taatgtgtaa taattaggtt tttcagtttg tgcagtatcc attttatact
     8881 gattattatc tttccaaatt ttttgccatt tcgattctac ttctttaaaa ttatatacgc
     8941 tcatattttg cctcctattt ttaattgtgt ataactacct tatttagtat tatttactta
     9001 taatttccaa ataattcttt gaaaataaaa aatcctcgtc actaaactta cgtttaggga
     9061 cgaggataaa tcttcgcggt accaccctaa ttagcctact acatatatag actctcttag
     9121 atagctttta atccagccat ggagaataga ttttaatcta gtaagttcaa agttatataa
     9181 tattagttct caccttccac taactctctg aaaaaatata atcttttact gctctagtct
     9241 attcaattta atataatttt tgaaattttc taattataat aacatatatt atcatgttta
     9301 tgctaatgtg tcaatatcta tataaggtgc atctttccat aatttttcta aattataaaa
     9361 ccctctattt tcctcattaa atacatgaac cattatgtct ccataatcaa ggagtaccca
     9421 agtttgagtt ccttgtcctt ctttacctct tggctctaat cctagttttg ctaattcatc
     9481 ttccacattg tcagcaatag cttttacctg tctctgagaa gatgctgtag taataataaa
     9541 ataatcacac aaactagaaa cttttcctat atttattata actgtatctt gacccagttt
     9601 atcttctatg gcatcatatg ctatttttgt catttgttcc actgtcatat aaaaattcct
     9661 cactttccta attaaaatac acttagttat tgtaacatat taatacgatt ttgtaacata
     9721 gtcaaattta aatttattaa tatgtttgct tttatatagc ttttttataa taacttttct
     9781 ttaattagat agtttcttgc cataactgtt cttggatgta tctcttcttt tttattgatg
     9841 acaaacatta aagtattatt aaaagaagtt aaaagtgctt catcaagctt ccctccatat
     9901 gctaattctc ttaatgtatc tacaactggg aattttcttc cttcttcaat taaatcagct
     9961 atgtaaatta ttttttctaa aagagacatg tcctctctcc ctgttgtatg atatttgatt
    10021 gcatttataa tatcttcatc ttctacttca aattcatact cgcatatgta cgccccaatt
    10081 acactatgag acaaggaacg atttccatcc tctattgggt ctaaatatat ttcatactta
    10141 tttacataat cctctatttc cttgtcactt aaatactttg cacaatcatg aatcattcct
    10201 gctaagtaag ctttttcttt gtcacatcca taaatctcac ataatttttc agcacatttt
    10261 gcaacatttt ttgaatggct aagcctcccc actggtagca tttgatttag tctttggtta
    10321 atattttcta agttcattta atcatctcca tattgatata gtttattttc atataaataa
    10381 ttttctacat tttcgggtac taagtaacgt attgacttgc cttcatttag ctgttccctt
    10441 atatatgtgg acgatatatc cagtgatgga acataaactg ttataatgtc agcattatac
    10501 ttcttagtta gtttttctat agtctcttgt gaccttaaca aactaattcc tggtctagtt
    10561 gcggctataa aagtagctaa ctcaaagttt ttctttacat ctttccactc ttcaacacta
    10621 caaattgcat ctgcacctgt tataaaataa atgtctgcat ttttgtattt tttctttaag
    10681 tattttaatg tatctattgt ataagtcctt ttctttcttt cgatttctac ttttgaaact
    10741 aaaaaatctt cgttgctttc agttgctaga agtgtcatat tatatctatc atacttatct
    10801 gtagtgatac ataatttatg tggtggattt cctgatggta taaagattat tttgtcaatg
    10861 tcatacttgt ctctaataaa ttctgctgtt gctaaatgag catagtgtat agggtcaaaa
    10921 gtacctccca atatacctat tttgattttg cctttatggc gattaaattt ctctagtttt
    10981 tcagtatttt tcaactctgc catcttaact agattttcac ttctcatatt ttgtctcctt
    11041 ttaatgttat aatactattg taccattatt ttttatttct taaagcttat aatt
//
LOCUS       DAEMHW010000126        10994 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEMHW010000126 DAEMHW010000000
VERSION     DAEMHW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.126"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(359..1450)
                     /locus_tag="KPK67_002660"
     CDS             complement(359..1450)
                     /locus_tag="KPK67_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459605.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKEDMSEVKQEITVMKEDMSEVKQEITVMKEDMSEVKQ
                     EINVLKVDMSEVKQEMTVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEM
                     TVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINV
                     MKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIE
                     IKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKED
                     IAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(1680..2765)
                     /locus_tag="KPK67_002661"
     CDS             complement(1680..2765)
                     /locus_tag="KPK67_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG2459606.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2801..4114)
                     /locus_tag="KPK67_002662"
     CDS             complement(2801..4114)
                     /locus_tag="KPK67_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2459607.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAHVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(4115..6406)
                     /locus_tag="KPK67_002663"
     CDS             complement(4115..6406)
                     /locus_tag="KPK67_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2459608.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDTKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFVEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6396..7190)
                     /locus_tag="KPK67_002664"
     CDS             complement(6396..7190)
                     /locus_tag="KPK67_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG2459609.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(7229..7675)
                     /locus_tag="KPK67_002665"
     CDS             complement(7229..7675)
                     /locus_tag="KPK67_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG2459610.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7747..9153)
                     /locus_tag="KPK67_002666"
     CDS             complement(7747..9153)
                     /locus_tag="KPK67_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2459611.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(9171..10541)
                     /gene="hydA"
                     /locus_tag="KPK67_002667"
     CDS             complement(9171..10541)
                     /gene="hydA"
                     /locus_tag="KPK67_002667"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG2459612.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLETKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGTPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10674..>10994)
                     /locus_tag="KPK67_002668"
     CDS             complement(10674..>10994)
                     /locus_tag="KPK67_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG2459613.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKS
                     VYGK"
BASE COUNT         3193 a         2206 c         1143 g         4452 t
ORIGIN      
        1 aaatagtaat attattttta tagtaaaaat tttattcata ttactattta taatttttat
       61 agagcaattt tagtgccaat atttgcatac atttgtatag tataaaattt ttatgttctc
      121 attgataaaa ttttacttat ttcaagcgtt ctattagatt ttatcatcta aattttaggt
      181 tttattttta taaattaatt gtatttacat aaaaaaaaag acctaaaaac aaatttttaa
      241 cggtcttttc tcaatttttt attttcattt ctaaaattta taataaatag taagaacact
      301 ataaattctt ttatataaaa ttcttagttt gtggatgata aattagtgaa ttaaaatact
      361 actttactga ttttagtttg acaatatcac tccaattttt actagtggct aactctacta
      421 aatttaagtc tcttcttatt tcagctatat cctcttttat agaattagtg tcacctttta
      481 tatatgctat gttatgttct atattatcat gagtagcttt atttatttca gaattatgta
      541 ataaacttcc taaaatatta ttctgttcta tctgtatctc tttcattgat ttatcttcac
      601 attctagact atcaatctta atttcaattc catataattt ttcatttgct atggatactt
      661 cgtccttaat tccattcata tcttcattaa tgttatttat tttattttgc ataaagttta
      721 cttctgattt aacttcggaa acatcttctt tcattacagt tatttcttgt cttacttctg
      781 acatgtcttt tttcattaca tttatctctt gttttacctc tgatgtatct tctttcatta
      841 cagtcatttc ttgtcttact tcggacatat cttctttcat tatatttatc tcttgtctta
      901 cttcggacat atcttctttt aatacattta tctcctgttt tacttccgac atatcttctt
      961 tcattacagt catttcttgt cttacttccg acatatcctc tttcattgta tttatctctt
     1021 gtcttacctc tgacatatct tctttcatta tatttatctc ttgtcttacc tcggacatat
     1081 cttctttcat tacagtcatt tcctgtttta cttctgacat atctactttt aatacattta
     1141 tttcctgttt tacttccgac atatcttctt tcattacagt tatttcttgt tttacttccg
     1201 acatatcttc tttcattaca gttatttctt gtttcacttc cgacatatct tcttttaata
     1261 catttatttc ctgttttact tccgacatat cttctttcat tacagtcatt tcttgtttta
     1321 cttccgacat atcttctttt aacacattta tctcttgttt tacttccgac atatcatttt
     1381 ttatgctaac tacatcttca tttatagtat ataataactc taaaatcttc tcttgcactt
     1441 gcatcaccat ccctattaat tattataaca ttttacacaa atgctataca atgttaacat
     1501 atatatcaga attattaaaa ttttaattaa acaaaattga ttcactttat tacttcaaaa
     1561 ctaattaata tctaaaataa ctacaaataa acaaaaataa ggtcaacaac aaaaaaggct
     1621 tatctctttg agatagcctt tcttattttt atttaaaact ttgtttttta taaaataatc
     1681 tatacattca gctctgaaca tgaaattttt cttccaagtg cttttggttt acaaactgat
     1741 atacaaagtc cacaatatct acactcttct tcattcaatt ccatcttatc attcaatttt
     1801 ctcgcttgat atgcacaaac tctctcgcac atcttacatt tagtacattt ttcttcatca
     1861 taagtaaact caaagctaag tctttcatta acatctccca tatgattatg tactatacca
     1921 caagcatttt ctatagtatt atatcctaat ctattaagat tagattttaa atcatcatgt
     1981 attttagata ttacttgtgc gccttttaaa attactactg aacatatacc aacaaagtca
     2041 gcacctgcca taatcatttc taaggcatca tctccactta cacatccacc taagccaatt
     2101 attggtatat taacttgttt ttttatttca tatattctct gtaaagttat tggcttgata
     2161 gtttctccac ttaaatatcc tattccattt ccacctaata aaggttgccc tgtttctatg
     2221 tctattctaa atgctggtcc tacagagtca caagcagtta ttgcatctgc tcctgcttct
     2281 tctaattttt tagcaaaatc gcctatctca tctatcatag gaggaagttt aactattaca
     2341 ggtatattta ctctttcctt tgcatcttta agcattggta tcaaatctct atagtcataa
     2401 gatactaatt caataaagtc agctcctgca tttgcaactt tctctactaa ttcgctactt
     2461 tcttcaattg tatgacctac tgaagctata cagacagtcc catctctttt catttgtggt
     2521 atttcaaaat ctatccattg ctctggctca aaatctgtcc atttttcatt atttaaaaga
     2581 gcattagctg tatttctaac catatgtggt gctggattta tagctctttc ttttcttata
     2641 gtcttagtca caactgcccc tgctcctgca tcacttaata tcttgcatcc agctgcacta
     2701 tatgttaatg gtcctgaacc tattataagt ggtgatttta attctttacc taaaaaatta
     2761 tacattatct gcttcccctt ttcatatata tttacattcg ttaaaatttc caaaattcac
     2821 aagcaacttc tttcatttct ttaacaatag cttttttatc taaatcaact aactgttttt
     2881 cttttaaaat atgtctacca tctatgtata catttgatat aaattctttt ttaccctgaa
     2941 ctactatctg gtcaaatata ttatcaagag tcacaggagt tttaaattca tcttccataa
     3001 ctattatatc agccttatta ccaactgcta ttttaccaga attttgaagt cctaatacat
     3061 gagcaccatg ctctgttgcc attctaaata ctaaatttgc tggcattact gatgcatctt
     3121 cttcaactga tttatgcatt aaaaatgcac ctctcataac ttcaaaaaaa tcatttatat
     3181 atccgtcagt acccagtgcc acttttatac cttttttaat catttttggt actggagaaa
     3241 aacctccacc tacttcacag ttgctaagag gcatatgaac cacttttaca cctttttctt
     3301 ttaatatatc tatttcttca tcatttactt ttacacactg agatgctaat acagtttcat
     3361 ccaatatatc taaatcatta tagtagtcaa ctgctttttt gccaaaatgt ttttcagcat
     3421 agtttggctc atatatactc tcacataaat gaaattgcat tggtgcattt aatttttttg
     3481 catcttcttt agctttctta ataaatctat cagaacatgt gaagcttgta tgtgtacaca
     3541 ttattccatt tattaattta ctattttctc tactccactt tattaaatta gaattttcat
     3601 ctaaacatct aagtccattt tcaaagctta ttctttcaca actttctaag gataaaatcg
     3661 ctttcatgcc tatttcttct agtatttttc cttgctcaat taaagttcct tcttctgtat
     3721 ttggagcttc tagggtatca caaaaagcta ccacaccaga ttctataagt tctattgctg
     3781 atgctttagt tgttgccttc acttctttca atccaattct attttctata aatggccacc
     3841 aatagtcatt taagaaacct tcaaagtcag taaaatgtac atttgccggt attcccctag
     3901 aaagaactcc atattgatgc atatgaccat tgacaaatcc tggcattatt atagcatctg
     3961 atttatctat tatttcttca atatttttat attttatttc aacttcttca tttggcaata
     4021 tatcttttat aatgtcattt tctactacaa cagcagaatt tgtatatatt tcatcagcag
     4081 aagatattaa gtatttactt ttaatagcaa tcatttattt acctccctga tttaattttt
     4141 tcatggccat ataaactttc tcagaagtta taggaagttc tcttatacga actcctgtgg
     4201 cattgtatat tgcatttgct attgctggtg caggtgtatg aataactatt tctcctaaag
     4261 atttagctcc atatggacct gttggctcat aattatctac aaaatcaaca attatgtttc
     4321 ctatatcttt ttttgttgga actttgtatt gtaatagatt agaagtttga agctttccat
     4381 cagaaccatg tctagggtct tcatacattg caagccctat accttgagtt acaccacctt
     4441 caacttgtat tctagcaagt gcaggattca taactgtacc gcaatctgta gaacaagcat
     4501 aatttattac tttaaactcc cctgttgttt tatctaactc tatttcaaca aatcctgcta
     4561 agaatggttt agcactctct tgtataccat aagattcaga tgaatataaa acttcttgat
     4621 ttccaccacc aacagattct tttcctaatt cacttaacaa aacaaacttc tctacatctt
     4681 cactataaca aactctgtct tcatatagta ttaaatcatc tattggtaaa tcgagtttct
     4741 tctgtgctac ttttaatatc ttttgtttta aactttcagc agattttatt gtagcatttc
     4801 cagttacata agtagtacaa gaagcgtatg caccagtatc atatggacac atatctgtat
     4861 ctcctgtata aacggaaatt ctatcaatag atgtattcaa tgcttttgca gctatttgtg
     4921 caagtattgt gtcagaacca gttcctaagt ctgaagaccc agaaaacaac ttataagttc
     4981 catcttcatc catcctcata tttactattg ccatgtctat gttggctata ccagaaccat
     5041 gagttcccac agacattcca acagccctca ccttattgtt tccaatatcc ctaacagggt
     5101 acttttcatc ccatccaata agttttttac ctctaattat acattcatca agtgcacaac
     5161 ttcttattgg aaaattcatt attccgcctt cagtttgttt ttttataata ttctttaact
     5221 ttatttctgt tgggtccata ttaagtttat gcgctaattc atcaattgca caatcaagtg
     5281 caaatgtacc ttgtgttgct ccatatcctc ttaatgctcc tcctggaact aaattagtat
     5341 atacagttct cccatcaaat tttattgcag gtacattatt atatgttgga agtacattat
     5401 gacctgattc agagcaaact gaaggtccat tatcgccata agcgcctgta ttgttcaatg
     5461 ctttcatatc tatagctttt atgtttcctt ctttatctgc cccaattttt atatcaaaaa
     5521 acatctgatg tctagtatta gtcattgcaa aagtttcttc tctagtataa attagttttg
     5581 ctggtctttt tgtcatccaa gttacaaatg atgcatatat ctctgttaca gctatgtttt
     5641 ttccaccaaa acctccacct attcttggtt ttattactct cacttttgaa agaggtattc
     5701 ccagtgcttt agaaacctgt cttctcatat gataagctga ttggtttgca gaagttacta
     5761 ctaatctgcc attcgtatct atatacgtat aagctctatg agtttccatc atacaatggg
     5821 cttgtgattg agtcatataa gaatgttcta ctattacatc actttcttta aactcttttt
     5881 ctacatcccc tttacccatt ttttctctcg atacaatatt ttttgtagca tccaatccaa
     5941 aatcaaatgg acaaaacaaa tcatcttctt catggattaa aattttattt ccttccgatt
     6001 ctcttgcatc caatatagga gtcattactt catattcaac ttttataagt ttcatagctt
     6061 taatagcagc cctttcatct tcagcggcta ttatagcaac ttcatctcct acatatctaa
     6121 ctacgtcttc aagtattaat tggtcatgag gtgatgcttc tggatatgac tctccaacca
     6181 ttgaatatct ataatttgga acatctttgt aagtgaatat gtttactaca ccttttacct
     6241 ttaatgcatt ttttgtatct atgtttttta tcttcgcaaa agcatgtgga cttctaagaa
     6301 gcttaattgt taaaacatct ttatgaaaaa ttaaatcttc tgtatatatt ggttttccag
     6361 taacaatggc ttcactatct atttttctta cctctttatt taccatcaca ctcacctcca
     6421 atactattat acattctcac tactaaggct tttgccatat cttttctgta ttctttactt
     6481 cctcttatat tacttccaaa tgaaagttct tcacttgata cttcaccagc tttttctata
     6541 tcattttcta aacttaatat ttcacttgct ttataggcaa tttttgctct tcttggtcta
     6601 gcgcctattg caattttata aatacttcct tcttttaaca ttgctccatt taatactgca
     6661 aaatcacctg tacattttct tatagaatca aatacaccta tagcatcttt tttaggtaat
     6721 ataacttcta ttaatatatc tttttcaagc tcactttcta aaaaatcagc taagttcata
     6781 accccttttt tatataactt aacctttgca tctaccacta gtaatgaagg tattaaatct
     6841 gaaaatccat acttagcaaa tacgctagct cctactctag ctccacttct aaattgaact
     6901 cctactatat tagaaacagc gtttgaaatt accccattac agtaattttt aataacctta
     6961 tttaattcta aagttcttaa acttgtgtct gctccaatta atatgctatc atcagtttca
     7021 tttatataat ctaaagctaa gtcttttaaa tctattgctg agccaatatt tttactacct
     7081 agttttaaaa atccacatcc tccaagtatt atgttgtttt ttcttgatat tagcagttca
     7141 taagcttcat ctaaactttt tggaactaca tattccttta tagttatcat actctctccc
     7201 catatcaact taattttcac ttaaaagtct aagtgtttaa atatttatgt attgctctta
     7261 attgaccttt atatcctgta catctacaaa ggttaccatt tacataatct ataacttctt
     7321 cttctgttgg attttgaagc tctcttttca ttgccaaaac catcattata aatccaggag
     7381 cacaaaatcc acactggtca gccccttcgt ctgcaagaaa atctccaaac ttctttgctt
     7441 catccaagac accctcaagt gttgtaatat gctgaccttc tactctagca gtaagataat
     7501 tacatgaaag cacggccttg tcatttacca tcactgtaca taacccacat gagcctgtat
     7561 cacacccttt tttaacactt acatatccat tttctcttaa tgtatctgct aaaaaatcat
     7621 ctggtcttat acttaattct ttttctttat tatttatggt acatttgata atcaaataaa
     7681 tcacctcaaa actcatattt ttaagctaaa gttttactat acttttttat gaataaatta
     7741 catttattaa taaaggctat tccatttatg accttccatt ctatttttta tagttcctct
     7801 tacacaaagt ctctcagctg tatcttgagc atcttttaat gctttctctt cattagctgt
     7861 caatattttt gaatcttcca ttattatatt tccatctact ataacactct ctatattttt
     7921 catactagaa gaatatacta atgtagatac cggattatgc ataggtatcg cttttggtga
     7981 aagcattgga ttaaatatta gtaagtctgc ttttttacct atctccaatg aacctatctc
     8041 atcttccatt ccaatagctc ttgctccatc tatagttgcc aactcaagaa ctttttctgc
     8101 tgacattgct aatgggtcac acttattaac tttatgttga agtgctgtta atttcataag
     8161 ttcaagcata tcttgagagt tattactagc ggctccatct actcctaaac taactgttat
     8221 tcctttttta agcatttctg gcactggtgc aacacctgaa gataagtaca tattacttgc
     8281 tgtattatga gacactttca tatcgtattt tttagtaagt tccatatctt tttcagttag
     8341 atacacacaa tgtaccataa gcacatttgg tccaagtatt cctaattttt ctaaaacatc
     8401 tatgtcatat tgcccatgca attcttttgc tgcttctcta tcaaaaggag tctctgatat
     8461 atgtactgta aatagagcat catcatattc ttttactact ctccatagca tttcaagcat
     8521 ttcttgagaa tttgaccata ttgctgcagg agctactcct attttgattc ttccattttc
     8581 tgtattatgg tgtttttcaa acaatcttct tacatctttt tcaaccgttt ctacatcttg
     8641 cattattcca ggatgaactc caaattgagc tcctgtattc atacatcctc ttccaagtat
     8701 tcctcttatt cctaattctt tataagcatc tattattcca tcacaaagac ctggcttact
     8761 atgaggatac atgtagtcaa ccattgttgt tataccactt ctaagtcctt caatacatcc
     8821 aagcattgca gcatcataag tatctttggg ctctaaatag ttagctgctg gaaatgtcat
     8881 agtttctaac cagtctttaa gtaccatatc atctcccaat ccttttaata aagtttggaa
     8941 taaatgatta tgtgtattta taaaacctgg gaataaaact ttacctttag catctataat
     9001 ttttttaaca tcagtatatt tactttctat ctctttagaa tttccaatat cagcaatctt
     9061 gttatctttt actactaatg ccccatcaaa aataacttct ctctctttat tcacagttac
     9121 tattatggca ttcttaataa gtatatccat cattcttatt tccccccgca ttatttagta
     9181 atttttcttt taatatattg acctgttgga tgttctttaa cattacaact ttgtactaca
     9241 tgatttcctc tcaaaaatac atcttcaact ttacatttaa ccttaaatcc ttcatatgga
     9301 gtataatcta cattttgttt ttgagtttta taactaattg tttcttcttt attgatattt
     9361 aaaatgacta tatctgcatc acttcctaca gctatctcac cttttttagg atacattcca
     9421 aatatttttg cagcattcgt agaagtaact tccacaaact tatttacact tattctattt
     9481 tctaagaccc catatgtata taaaagtgct aacctatgtt caacaccagg tgttccattt
     9541 ggtatcttac taaaatcatc aattcccaag tctttttgcc ccttaaaatt aaatgaacag
     9601 tggtctgtgc caactgtttg aatatctcca ctagacaatc cttcccaaag gtaatttatg
     9661 tcttcttttt ttcttaaagg aggagacatt acatacttag caccctcaaa tttatcctta
     9721 ttatataaat tgtcttctaa taatagatac tgtgggcatg tttctactat catatctatg
     9781 tttttttctc tagccatttt tacagtttca agtgactccc tacaacttaa gtggactaca
     9841 taggattttg aattagataa tttagttata tcagcaagtc ttgaaacaga ctctttctca
     9901 actatgttag gtcttgtaag tggatgatat tttgtctcta aattaccatt ttgtatattt
     9961 tcttctatca atacatctag taaatcccca ttttcacaat gaaatccaac tatacaacct
    10021 aaatcccttg ctttctttaa aaccctgtat attgttccat catctacttt catgccatca
    10081 tatgccatat acatcttaaa agatactatc ccatccgaaa taagtttttc catatgatta
    10141 aatgtatctt tatttaaaca tgtaattgtc atatgaaaac cataatcaca ataacaattt
    10201 ccacttgctt ttttatgata tgcttctaca ccttgcagta gattttcccc ctcattggct
    10261 tctgcaaaat ctagtattgt agtagttcct ccagctatag ctgctcttgt tcctgtttca
    10321 aaattatctg cagttgtaat agaacctgca ttcatatcaa aatgagtatg tgtgtctatt
    10381 ccaccaggaa ttacaatttt acctgtagca tcaataacct tatcaccatt tatttctaag
    10441 ttatttccta tttcaactat cttttcatct tctatcctta aatcacttat ataagtctta
    10501 tcagaggtaa ttatagttcc acctcttagt attgttccca tatatacctc ctaactttta
    10561 agctttaaag tattttctaa gtattgtaaa actttattta aaattgtatt ttctaaaggt
    10621 gctatattca gcacctctag actatattta aataatttat tgtgaactaa atattatttt
    10681 ccgtatacag attttaatat tccatagtaa ccttctgttc ctataaataa ctgttcttgc
    10741 tctatatact catctattgt gtgagcaaga ttttccttag aaggaccaaa tcctatagtt
    10801 tttattccag cttcacctgc atagtgactt ccattcgtac agaatgagta ttgagttatt
    10861 tctgaatcta ttccagcttc ttttaatccc ttatatgctg cttgaacaaa ttcatcttct
    10921 tcgtcatata accaacctgg gaagaatctt tcactttcta tagtatttcc tgtatagcaa
    10981 tcagcttttt caac
//
LOCUS       DAEMHW010000127        10868 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEMHW010000127 DAEMHW010000000
VERSION     DAEMHW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10868
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.127"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            131..3847
                     /gene="rpoB"
                     /locus_tag="KPK67_002669"
     CDS             131..3847
                     /gene="rpoB"
                     /locus_tag="KPK67_002669"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBG2459614.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            3889..7374
                     /gene="rpoC"
                     /locus_tag="KPK67_002670"
     CDS             3889..7374
                     /gene="rpoC"
                     /locus_tag="KPK67_002670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBG2459615.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            7663..8085
                     /gene="rpsL"
                     /locus_tag="KPK67_002671"
     CDS             7663..8085
                     /gene="rpsL"
                     /locus_tag="KPK67_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBG2459616.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            8209..8679
                     /gene="rpsG"
                     /locus_tag="KPK67_002672"
     CDS             8209..8679
                     /gene="rpsG"
                     /locus_tag="KPK67_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBG2459617.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            8728..10794
                     /gene="fusA"
                     /locus_tag="KPK67_002673"
     CDS             8728..10794
                     /gene="fusA"
                     /locus_tag="KPK67_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG2459618.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         4195 a         1395 c         2285 g         2993 t
ORIGIN      
        1 tcaaaaataa tgcatagttt acatataaga taaaaaatat attgaaaaat acttgacatt
       61 atgctaaaat tattagatgc gttagaatga actattgttt tatttttaat tattgagagg
      121 tgaaaagtga atgccacatc ctgtcacgat aggtaaaaga actagaatga gcttttctaa
      181 aattaaggaa atagctgatg tgccaaatct tatagaaatt caagtagatt cctatgagtg
      241 gtttttaaaa gaaggtttaa aagaagtgtt tgatgatatt tcaccaatag aagattatac
      301 tggtaatctt atattggaat ttgtcgacta ttctctagat gataaaccaa agtacgatat
      361 agaagagtgt aaagagagag atgctacata ttgtgcacct ctaaaggtta aggtaagact
      421 tataaataaa gaaacaggtg agataaaaga acaagaagtg tttatgggtg acttcccttt
      481 aatgacagaa agaggaactt tcgttataaa tggagcagaa agagttatag taagtcaatt
      541 agttagatct cctggtgttt actatgcgga agaaagagat aaaacaggta agagattgat
      601 ttcatctact gtaattccta atagaggagc atggttagaa tatgaaacag attctaatga
      661 tgtaatatct gtaagagttg atagaacaag aaaacaacca gttacagttt tacttagagc
      721 tttaggaata ggaacagatg cagagataat tgacctttta ggagaagatg agagattatc
      781 tgctacatta gaaaaagata atactaagac agtagaagaa ggtcttgtag agatatacaa
      841 aaaattaaga ccaggtgaac ctcctactgt agaaagtgca tcatctttat taaatgcttt
      901 attctttgac ccaaaaagat atgacctagc aaaagttggt agatataaat tcaataagaa
      961 acttgcttta tgttatagaa taatgaataa aatttcagca gaagacataa taaatccaga
     1021 aactggagaa gtatttgtaa aagctggaga aaaaataagc tatgatttgg ctaaagccat
     1081 acaaaatgca ggtataaatg tagtaaatct actaatggat gatgataaaa aagttagagt
     1141 tataggaaat aattttgtgg atattaagtc tcacattgat tttgacatag atgatttaaa
     1201 cataaaagaa aaagtacatt atccaacttt aaaagaaatt ttagatggat atagtgatga
     1261 agaagaaata aaagaggcta taaaatcaag aattaaagaa cttataccaa aacatatatt
     1321 attagatgat ataatagctt caataagtta tgagttcaat atattctata atataggaaa
     1381 tattgatgat atagatcacc taggaaacag aagaataaga tctgtaggtg aattgttaca
     1441 aaatcaagtt agaattggtc tttcaagaat ggaaagagtt ataaaagaaa gaatgacggt
     1501 tcaagatatg gaagctataa cgcctcaagc gttagttaac ataagaccag tttcagctgc
     1561 aataaaagaa ttttttggta gttctcagtt atctcagttc atggatcaaa caaatcctct
     1621 atctgagtta acgcataaga gaagattatc agcccttggg cctggaggtc tttcaagaga
     1681 aagagctgga ttcgaagtgc gtgacgttca ccattcacac tatggtagaa tgtgtccaat
     1741 agagactcca gagggaccaa atatcggtct tataaactct ctaggaactt atgcaaaaat
     1801 aaatgaattt ggatttatag aatcaccata tagaaaattt gataaagaaa catcaacagt
     1861 tactgatgaa atacattatt taactgctga cgaagaagat ttattcgtaa gagctcaggc
     1921 aaatgagcca ttaacagaag atggtaaatt cgtaaaccat agagttgttt gtagaactgt
     1981 aaatggtgct gttgaaatgg ttcctgaaag tagagtagat tacatggata tatctcctaa
     2041 gcaggttgta tctgttgcca ctgctatgat acctttctta gaaaatgatg atgccaaccg
     2101 tgcccttatg ggagcgaaca tgcagcgtca ggcagtgcct ctagttagaa gagaagcacc
     2161 aattatagga actggtatag aatacagagc agcaaaagac tctggagcag ttgttgttgc
     2221 tagaaattct ggtatagcag aaagagtaac agcagatgaa ataattataa aaagagaaga
     2281 tggaaacaga gatagatata atctacttaa gtttaaacgt tcaaactcag gtacttgtat
     2341 aaatcaaaca cctataataa ataagggtga ccagataata aaaggtgatg ttatagcaga
     2401 tggtccagca actgatttag gagaagttgc acttggaaga aactgtctta tagcattcat
     2461 gacttgggaa ggttataact acgaggatgc tatattaata aatgaaagat tagttaaaga
     2521 agatagatta tcaacaattc atatagaaga atatgaatgt gaagctagag atacaaaact
     2581 agggccagag gaaataacta gagatatacc taatgttgga gatagtgcaa ttaagaactt
     2641 agatgacaga ggtataataa gaataggtgc agaagtagac tcaggtgata tattagttgg
     2701 taaagtaact ccaaaaggag aaactgaact tactgctgag gaaagattac ttcgtgcaat
     2761 atttggagaa aaggctagag aagttagaga tacttcactt aaagtgcctc atggtgaatc
     2821 tggtataata gttgatgtaa aagtatttac tagagaaaat ggagatgatt tatctccagg
     2881 agtaaatgaa ttagtaagat gctatatagc taaaaagaga aaaataaaag ttggagataa
     2941 aatggctggt cgtcatggta ataaaggggt tatctcaaga gtattacctg aagaagatat
     3001 gccattcatg gaaaatggaa caccactaga cataatactt aacccacaag gtataccatc
     3061 acgtatgaac attggtcagg ttttagaagt tcatttagga cttgctgcta agacattagg
     3121 atggtatgta gcaacatcag tattcgacgg tgctaatgag tatgacataa tggatgctct
     3181 tgaagaagct gggtatccta gagatggtaa attaacttta tatgatggta gaacaggtga
     3241 gtcttttgac aacagaataa ctgttggata tatgtactac ttgaaactac atcacttagt
     3301 agatgaaaaa ctacatgcaa gaagtactgg accatactca ttagttactc aacagccact
     3361 aggtggtaaa gcacaatttg gtggacaaag atttggagag atggaggttt gggctctgga
     3421 agcatatgga gctgctcaca tacttcaaga aatacttact gttaagtctg atgacgttgt
     3481 aggtcgtgtt agaacatatg aagctatagt taaaggtgaa aatatacctg aaccaggaat
     3541 cccagaatca tttaaggttc ttataaaaga acttcaaagt ttatgcttag atgtaaaagt
     3601 attgacagat gaagaccaag aaatagaagt aagagaatct gtggatgaag atgatacaat
     3661 aggtgaattt gagctagatg ttgtaaatca tatgggagaa gttgaagaaa gtaatataat
     3721 agaagaaatt gaagatgatt ttgcagaaaa tgcagaagat gaggatatag agaatttaga
     3781 agaattcact gaagatgatt tatttgaaga agaaatagat tttgatagtg atgactttga
     3841 tatgtaaatt taattaatgt aaatctcaaa tagaagggag agaactcctt gtttgaatta
     3901 aacaatttcg agtcgataaa aatagcattg gcttctccag aaaaaataag acaatggtct
     3961 aggggagaag ttaaaaagcc agaaactata aattaccgta ctttaaaacc agaaaaagat
     4021 ggtcttttct gtgaaagaat atttggacca caaaaagact gggagtgcca ctgtggtaaa
     4081 tatagaagag ttagatataa aggtgtagtt tgtgatagat gtggagtaga agtaactaaa
     4141 tcaaaagtaa gaagagagag aatgggacat atagagctag ctgctcctat gtctcacatc
     4201 tggtacttca aaggtatacc aagtagaatg ggacttttac ttgatatgtc accaagatcg
     4261 ttagaaaaaa tattatactt tgcctcatat gtggtagttg atccaggaga gactggatta
     4321 aacgaaaaac aattgcttac agaaaaagaa tacagaactg ctcttgaaaa gtatggatat
     4381 acttttactg taggaatggg tgctgaagct gtaaagacat tactacaaaa tatagattta
     4441 gagcaacaaa gtaaagacct aagagcggag ttaaaagata gtacagggca aaagaaagtt
     4501 agaacaataa gaagattaga agttgtagag gcatttaaaa agtctggaaa taaaccagaa
     4561 tggatgattt tagatgcaat accagtaata ccaccagatt taagaccaat ggtacaactt
     4621 gatggtggaa gatttgcgac ttcagaccta aatgatttat atagaagagt tataaataga
     4681 aacaatagac ttaaaagatt attagagctt ggagctccag atataattgt aagaaatgaa
     4741 aaaagaatgc ttcaagaagc tgttgatgca ttgatagata atggtagaag aggtagacct
     4801 gtaacaggac ctggaaatag accacttaaa tctttatcag atatgttaaa aggtaagcaa
     4861 ggtcgtttcc gtcaaaactt acttggtaag cgtgttgact actcaggacg ttctgttata
     4921 gttgttggac cagaacttaa attttatcaa tgtggacttc caaagaagat ggcattagaa
     4981 ttattcaagc catttgttat ggataaatta gttaaagaag gttatgcaca taatataaaa
     5041 agtgcgaaat ctatagtaga aaaagttaag ccagaagttt gggatgtttt agaagatgtt
     5101 ataaaaagtc atccagttct tcttaaccgt gcaccgactc tacatagatt aggtatacaa
     5161 gcatttgaac caatcttagt tgaaggtaag gctataaaac tacatcctct tgtatgtaca
     5221 gcttataatg cagactttga tggtgaccaa atggcagtcc atgtaccttt atcagtagaa
     5281 gcacaagcag aagcaagatt cttaatgctt tctgtaaata atatacttgc tcctaaagat
     5341 ggttcaccta taactactcc atctcaggat atggttttag gttgctatta tctaacaata
     5401 gaagcccaag atggagctaa aggaacaggt atggtattta aagactttaa tgaactatta
     5461 cttgcttatt acaataaatc agttcatcta catgcattag tgaaactgaa ggtaacactt
     5521 gaagatggaa gaagttcatt ggttgaaagt actgttggta gatttatatt taatgaaaat
     5581 atacctcaag acttaggttt tgtagataga aaagaaaatc catttgcact tgaagttgat
     5641 ttcttagcag ataaaaaatc tcttggtaaa ataatagata aatgctttag aaaacacgga
     5701 aatacagaaa cagctgaatt actagattat ataaaagctc taggatttaa atattctaca
     5761 ttaggtggta taacagttgc tgttgatgat atgagtgtcc cagaagaaaa gaaagtattt
     5821 atagctgagg cagaagctaa agttgataag tatgaaaaag catacagaag aggtctaatc
     5881 tctgatgaag aaagatatga aaaagttata gagacatgga cagaaacaac tgataaagtt
     5941 actgatgctc ttatgggtgg actagataga ttaaataata tatatataat ggcacattca
     6001 ggagccagag gttctaaaaa ccaaattaga cagctggcag gtatgcgtgg tcttatggca
     6061 aatgcatctg gtaaaacagt tgagatacca gttaaatcta atttccgtga aggtttatca
     6121 gtactagaat actttacatc ttcacatggt gccagaaaag gtcttgccga tacagctata
     6181 cgtacagctg aatctggata tttaacaaga agacttgttg atgtaagtca agatgttatt
     6241 gtaagggaaa tagattgtgg aacagaagat actacagaga tttatgctat aaaagaagga
     6301 aatgaagtta tagaagagat atatgataga attgtaggaa gatatactat agaccctata
     6361 ttaaatcctg aaactggaga agttatagtt gaggctgatt caatgataca agaagatgaa
     6421 gcagaaacta tagtagcttt aggaattgaa aaaattagga taagaacagt tcttaactgt
     6481 aaaactaatc atggagtttg ttctaagtgt tatggtagaa acttggcaac aggaaaagaa
     6541 gttaatatag gtgaagcagt tggtataata gcagctcaat ctatcggtga gccgggtact
     6601 cagcttacaa tgcgtacatt ccatactgga ggagttgcag gagctgatat aactcaaggt
     6661 ctaccaaggg ttgaagaatt atttgaagct agaaaaccaa aaggattagc tgtaataact
     6721 gaagtatctg gtagagttga aattgatgaa acaggaaaga gaaaagaagt aaatgtaata
     6781 ccagaagaag gcgagactca aacatatgta attccatatg gttctagatt aaaagttaag
     6841 caaggtcaaa tgctagaggc tggagaccct ctaacacaag ggtttataaa tcctcatgat
     6901 atagtaagag taaatggtgt taagggagtt caagaatata tagttaaaga agttcaaaga
     6961 gtgtatagac ttcaaggggt tgatgttaat gataagcata tagaagttat agtaagacaa
     7021 atgctatcta aagtaaaagt tgaagaccca ggagatacag atttattgcc aggtggatat
     7081 gaagatgtat taacattcaa tgaatgtaat aaagatgcta tagataaagg tctaagacca
     7141 gcagttgcta aaagagtttt acttggtata actaaagcat ctcttgcaac tgattcattc
     7201 ttatcagcag cttctttcca agaaacaaca agagtattaa cagaggcagc aataaaaggt
     7261 aaagaagacc acttaatagg acttaaagaa aatgttatat taggtaagtt aataccagca
     7321 ggaacaggaa tgaagaaata tagaaatata gctgttgaaa aaattgaaga ttaatagcat
     7381 atagagtaaa gaaatcttga caaccattat ttgtggtgtt aaaatagtaa agtgtgttgt
     7441 ttaaaagtac actaagggct tgaacaagtt cttagtgact tttagatggc aataaatgag
     7501 aactaaaaag catcaagcta gaaaagtgct aatatcacat atttatgtgt tatagacatt
     7561 aaattgtaag taacgaatgt tacaatcagt cagtatgaat attcatattg atgtagttgg
     7621 cggtcactga taagatcgaa aatttggaag gaggtgcaga tgatgccaac aattaaccaa
     7681 ttagttcgta aaagtagaaa agcgctagag aagaaatcta ctgctccagc attacaaaaa
     7741 ggttacaact ctttaaataa gaaagtaact gacgctagtg ccccacaaaa aagaggggtt
     7801 tgtacttcag taaaaacagt tactcctaag aagcctaact cagctttaag aaaagttgcc
     7861 agagttagat taactaatgg tatagaagtt tctgcttata taccaggaga aggacacaac
     7921 ttacaagagc atagtgttgt tcttataaga ggaggaagag taaaagacct tccaggggtt
     7981 agataccata tactaagagg tactctagat acagcaggtg ttgataagag aagacaatca
     8041 agatctaaat acggtgcgaa gagacctaaa gaagctaaaa agtaaaaaca attaatgatt
     8101 gattatagtt cggtgcttta aatatatata tatttacggc atcacggcat taaatgattt
     8161 atgtcgagta cctatgattt aagttaaata attaaggagg gaagcgaaat gccaagaaaa
     8221 ggtaatattc caaaaagaga agttttacca gatcctatgt atgggagtaa ggtagttact
     8281 aaattaataa ataatctaat ggtagacgga aaaaaaggaa agtctcaaag aatagtttat
     8341 gatgctttcg caattgttgc tgaaaagaca ggagaagaag ctttagaagt atttaataaa
     8401 gcaatggata atataatgcc tgttttagaa gtaaaagcaa gaagagttgg tggggcaaac
     8461 taccaagtgc caatcgaagt tagacctgaa agaagacaaa cattaggttt aagatggcta
     8521 gtaaaatata ctagagctcg tggagaaaaa ggaatggttg aaaagctagc taaagagata
     8581 atggacgcag ctaacaacac tggagcttca gttaagaaaa aagaagatac tcataaaatg
     8641 gcagaagcta ataaagcatt tgctcattac agatggtagg atatatagat atcttataaa
     8701 attgaggata tcataaggag gataaccatg gctagaaagt ttcctttgga aagaactagg
     8761 aatataggaa tcatggctca tatagatgca ggaaaaacta ctactacaga aagaatcctg
     8821 ttttatactg ggcaaactca taaaatagga gaaactcatg aaggagcttc tcaaatggac
     8881 tggatggagc aagaaaagga aagaggtata acaataactt ctgccgctac tactgcttca
     8941 tggaaagacc atagaataaa tataatagat actcctggac acgtggactt cacagttgaa
     9001 gttgaaagat ctctaagagt tcttgacggt tcagttgctg tattctgtgc aaaaggtggg
     9061 gttgagcctc aatcagaaaa tgtatggaga caagctgaaa cttatggagt acctagaata
     9121 gcttttgtaa ataaaatgga tatattaggt gcagatttct acaatgttgt aagcatgatg
     9181 aaatctagat taaattctaa tgcagttcct atgcaattac caataggtaa agaagattca
     9241 tttataggta taatagattt attaaaaatg gatgctgtta tatacaaaga cgacctaggg
     9301 gttgaaatgg aagaaacaga tataccagaa gatatgaaag aattagctgc tgagtggaga
     9361 gaaaaattag ttgaatcagt agctgaaact gatgaagaat taatgatgaa ataccttgaa
     9421 ggtgaagaac ttactataga tgaattaaaa gttgctataa gaaaagctac tatagcttgt
     9481 gaaatgaacc cagttttctg tggtacagct tatagaaaca aaggtgttca gttagtaata
     9541 gatgcagttt tagattactt accagcgcca actgatatac cagctataaa aggtatatta
     9601 gcagatggag aagaagctga aagacattct tctgatgaag agccattctc tgcattagca
     9661 ttcaaaataa tgactgaccc attcgttgga aaactagcgt tcttcagagt ttattctgga
     9721 acattagaat caggttctta tgttttaaat gcaactaaga ataagagaga aagaattggt
     9781 cgtatattac aaatgcatgc taatacaaga gaagaaataa ctaaagtgta tgctggagat
     9841 atagcagcag cagtaggatt aaaagatact actactggag acacattatg tgaccctgct
     9901 aatccaataa tacttgaatc tatggaattc cctgagccag ttatatctgt tgctatagaa
     9961 ccaagttcta aagcagctca agaaaaaatg ggtatagctc ttcaaaaatt agctgaagaa
    10021 gacccaactt tcacagttaa gactgaccaa gaaacaggac aaactataat atctggtatg
    10081 ggtgaattac acttagagat aatagtagat agattattaa gagaatttaa agtagaagct
    10141 aaagttggtg caccacaagt tgcatataga gagactataa ctcaaccagt tgatgttgaa
    10201 tacaagtact ctaagcaatc aggtggtaga ggacaatacg gtcacgttaa gattagagtt
    10261 gctcctcaag agccaggtga aggatacaaa ttcacaaaca aaactgttgg tggatcagta
    10321 ccaaaagaat atgttggtcc agttgacatg ggtatacaag gagctatgca atctggtata
    10381 gttgctggat atccagttgt agacgttgct gttgagttat atgatggttc ttaccatgag
    10441 gttgactctt ctgagatggc gtttaagatg gctggttcaa tggcattcaa agatgctatg
    10501 aagaaaggta atgcagttct tttagagcct tacttcaaag tagaagttgt tactccagaa
    10561 gattacatgg gagatgttat gggtgactta aactcaagaa gaggattaat ccaaggtatg
    10621 gaagctagaa gtggtgcaca agttataaat gcattcgttc cactttcaga aatgtttgga
    10681 tactcaacag acctaagatc ttcaactcaa ggtcgtgcaa cttacacaat gatattcgat
    10741 cactatgagc aagttccagc ttcagttgct aagaaaatag ctgaaggaaa ataatttaaa
    10801 aataattaat aatataaata taaaaaataa tcagctagat gagagatatc tctcatctag
    10861 cacaagat
//
LOCUS       DAEMHW010000128        10773 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEMHW010000128 DAEMHW010000000
VERSION     DAEMHW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.128"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(307..924)
                     /locus_tag="KPK67_002674"
     CDS             complement(307..924)
                     /locus_tag="KPK67_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBG2459619.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(1051..2073)
                     /gene="argF"
                     /locus_tag="KPK67_002675"
     CDS             complement(1051..2073)
                     /gene="argF"
                     /locus_tag="KPK67_002675"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBG2459620.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYKARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            complement(2182..3369)
                     /locus_tag="KPK67_002676"
     CDS             complement(2182..3369)
                     /locus_tag="KPK67_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBG2459621.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLCSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            complement(3399..4259)
                     /gene="argB"
                     /locus_tag="KPK67_002677"
     CDS             complement(3399..4259)
                     /gene="argB"
                     /locus_tag="KPK67_002677"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBG2459622.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGSEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            complement(4274..5491)
                     /gene="argJ"
                     /locus_tag="KPK67_002678"
     CDS             complement(4274..5491)
                     /gene="argJ"
                     /locus_tag="KPK67_002678"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBG2459623.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            complement(5532..6566)
                     /locus_tag="KPK67_002679"
     CDS             complement(5532..6566)
                     /locus_tag="KPK67_002679"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBG2459624.1"
                     /translation="MNKIKVGIISATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     misc_binding    complement(6820..7033)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7251..8019)
                     /locus_tag="KPK67_002680"
                     /pseudo
     CDS             complement(7251..8019)
                     /locus_tag="KPK67_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
     gene            8319..8792
                     /locus_tag="KPK67_002681"
     CDS             8319..8792
                     /locus_tag="KPK67_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2459625.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTDVEQIVDGIRFTPMKLLI"
     gene            complement(9161..9718)
                     /locus_tag="KPK67_002682"
     CDS             complement(9161..9718)
                     /locus_tag="KPK67_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2459626.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
     gene            9976..10674
                     /locus_tag="KPK67_002683"
     CDS             9976..10674
                     /locus_tag="KPK67_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2459627.1"
                     /translation="MGKKYFKIHGVPSILWGNPSSKLYIYIHGQGGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEISVVEKFVEKFNCNLTIVENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT         3542 a         1835 c         1084 g         4312 t
ORIGIN      
        1 aaatcatatc cttcaataaa tattgttgat tcatcaaaat gcttggctga atcagttgtc
       61 aataaatatc taaaattagt taaataaatt tttcatgcac tatattataa aaaatatatg
      121 ttataagttt attttaaata gtatagactt ataacatata tcatagtttc ttgacaatat
      181 agaaattaac catgtatttc attatattaa taaaaaggat accaaaaatg gtatccttca
      241 taaatattta tatgtttatt tataaaaata atcatatatt tattacttta ctacacctaa
      301 acaatattat tcaagtgtat tcatagagtt agaaaattct tgtcctgctg taacaagatt
      361 agataacaat acatcagcta ctgcatcatt tactgaactt attatccatt tattatcctt
      421 tttagaacat tcaaactcta cattttttgt aacataagtc tctttaacgc ttttttgttt
      481 ttctttcata attgatacta acatttcagt tgttttctca tcagttaagt cttgtcctga
      541 aaaactcata ccaatcatct ttgtaaatgc ctcagctact atttcttgaa gtaatggtgt
      601 tgaatctata aatttacatt ctactttgat tgtagcctta tcacctttaa cctcgctgtc
      661 ttttattgta tatgtgattt tagatgcatt ctgttttaga taatctaaaa catattgggc
      721 atttgggtct tttgtatcta attcctttaa tatatcctta tatttttcgt cattattttc
      781 cattacctta ttcatgcctt caaagtcaaa ctttttcgca ctgttaaaaa aactatcaat
      841 tgtatcttct ggttttgcac ttgaacatgc tgttaaagat attgctaata aaaaagttgt
      901 taaagctaat atgatttttt tcatatctca aatcctccct cttaatttgt tgatacataa
      961 gttattttaa catataatga gagtttttga ataatatatt taaaatattt tataaatttt
     1021 cattaaagta aagtctatat taaaacatat ctatattaaa ttattgttca tatttacatc
     1081 agtcatagta gaaaccataa cagcttttat tgtatgcatt ctattttcag cttcttcaaa
     1141 tacctttgaa tattttgaat taaatacctc gtttgtaacc tctaattctg ataatccgaa
     1201 cttttcataa atctcctgag caaccttagt ttctaaatca tgaaatgctg gaagacagtg
     1261 taaaaatata acattttcat tttcagtttt ctttatcata tccatattta cttgataatc
     1321 tttaagttga tttattcttg ctttatattg ctcctcttct cccatagaaa cccatacatc
     1381 tgtatatata acatctgcac ctttaacatc atcaatattt tcagtcaaca ttatatctcc
     1441 tttggatttc ttggctattt ccttcatttc cttaaccaat tcttcatttg gccaaagttc
     1501 cttaggtgct agtgctacga aatgtattcc cattttagca catcctatca tcagtgagtt
     1561 tcccatatta tttctagcat caccaacata tacaaattta actttattca aatctttatc
     1621 tacattttca attactgtta aaaaatctgc cagtatttgt gttggatgat attcatctgt
     1681 aagtccattc caaacaggta caccagaata ttttgctaga ctttcaactg tttcttgttt
     1741 aaagcctcta aattctattc catcatagaa tcttcctaga acttttgctg tatctgatac
     1801 agactctttt ttacccatat gacaatcatt tggacctaga taagttacat gtgctccttc
     1861 atcatgagct gctacttcaa atgaacatct agttcttgtt gatgttttct caaataataa
     1921 agctatattt tttccttgta gcaaatcgcc ttttatacca acttttttct tagtttttaa
     1981 ataacttgat aaagttaata aatatctaat ctcttctttt gtaaaatcct ttaatgtcaa
     2041 aaaactctta ccttttaaac ttatattatt catattaatt cgccccttct tataaacttt
     2101 ttaaattcaa ttaaacttgt tagcacttta cttttttatt tacttctaat ttttatcttt
     2161 aatcaactat ttcttgaata tttagttaat ttctagtaat atatctttta ggatttcaag
     2221 agcctcatct atttccttat aactgattgt taatggtggt aaaaatctca atacattttt
     2281 tccagcagtc aaaataagca atcctttttt tctagctttc tcttctataa tactagattc
     2341 aatattagtt tcaattccta tcattagacc aatacctttt gtctttaata caactttgtt
     2401 attaaaactt tcaatagatt ttcttatata atttcctttt tctaatactt cattataaaa
     2461 accatcttta gatatctcat ctaatacaac tgaagctgcc accatagcta ataaattacc
     2521 tccaaaagtt gtaccttggt ctccttttcc tagtactttt gaaagttttt tatttaccaa
     2581 tactcctcct actggtattc ctgctcccaa cccttttgct acggtaacta tatctggttt
     2641 tacatcaaaa tattcatagc caaaaagttt accagttctt cctataccgc attggacttc
     2701 atcaaaaatc actacaatat ccttttcttg acatattttt actatttcca aaacataatc
     2761 tttttcaaga acattaactc caccttcgcc ttgaatcgct tcaagaatta ttgcacaaac
     2821 tgtggaatca agattatttt ttaaatcttc aatattattt ctttctatat atttaaaacc
     2881 ttcgggaaga ggataaaaat acttatggta tttgtccata ccagttgcca ttaaagacat
     2941 cattgttcta ccatgaaaag agtcttttaa acttatgata gttcctctac cattaccata
     3001 tttgtcacta ctatactttc ttgctatttt gaatgctgtt tcatttgctt ctgttccaga
     3061 attacaaaaa tatactttag ccattccact tgaattaatt attttctctc ctgaacacaa
     3121 cactggctca ttaaaatata aatttgaagt atgtaataat ttatctactt gctcttttag
     3181 agcattcttt aaattactat tactataccc aagagaagat actccatatc cacttgtaaa
     3241 atctatgtat ttattgccat tagtatcaaa aaaacaactt ccttcaccat aatctatgac
     3301 aaaattgggt tggttatatg tatctataaa atattcattc cactttgaaa ttgtattgtc
     3361 gttattcatt cttattctcc cctcttaaac ttataatttt attcattttc ttttcttatt
     3421 aatgtaccta ttccactatc agtaaatagc tctgttatta tagaatgtgg aactcttcca
     3481 tccaaaatat gtaccctatt tactccgtta tttaaggcat ctacgcatcc ctctatttta
     3541 ggtatcatac ctccagtaat tataccttct tcaaataatt tatcaacatc ctctaatata
     3601 acttctgtta ttaatgattt ttcttcatct ggctctctta acaaaccagg gacatctgta
     3661 agaagtatta atttatctgc atttaactct ttagctatag cagaagctgc tgtgtcacca
     3721 tttatattat aagtctctcc atcatctcca acacctatag ttgcaattac tgaaatataa
     3781 cctgacttta gacaagattc tataagctca acatttactt ttttgatttc tcctacaaat
     3841 ccaagttcat aatttttata tgggtcacat aaaatcatat tattatctat tccacaaagc
     3901 cctactgctt ttccaccttt tgtatgtatt ttatttacta agtctttatt aacctttcca
     3961 gcaagtacca tttttacaat ttccatggtc tcttcatctg tatatctaag accatttaca
     4021 aatttacttt ctatatccac ttttgctagc atcttattga tttctgaccc tccaccatgt
     4081 actaaaacta tattgattcc aacataactc attaacacaa ggtcttccat aacagattct
     4141 tttagaccat cttttttcat tgcacttcca ccatacttaa ctacaatagt ctttccctga
     4201 tgtttttcta tatatggtaa agcttctata agtgtatttg ctttttctat attaatcatt
     4261 aaagtatatc ctcctaattt ctataacttc catttattct tacataatca tagcttaaat
     4321 cacatcccca tgcatttcct ctggcattgc ccatatttaa atctactaat atacttattt
     4381 cgtcatcttc aaggattttt tttgcttttt cctcattaaa atctaatcca tttccatttt
     4441 tacatacttc aatatatcct ttcatactct caaaaataat atcaactttt tccatatcta
     4501 tttcttcacc tgcataacct aaagcacaca gtattctacc ccaatttgcg tcagctccaa
     4561 atacagctgt ctttactaat gaagaagata taactgtttt tgctaatttt actgcatgtt
     4621 cttcacttaa tgctccattt atatagcatt caattagttt tgtacatcct tctccatctt
     4681 ttgcaacaag tttagcaagt tctatgcaga catatgtaag tgcactcaaa aatacttgat
     4741 aatgttcatc ttttttgttt attctatcat tttttgcttt tccatttgct agaatcatta
     4801 ccatatcatt agtacttgta tctccatcca cactaactct attaaaactt ttgtcaacag
     4861 caatttttaa agcttctttt agcaaaactc catcaatatt tgcatctgta gttataaatc
     4921 ctaacgttgt tgccatatta ggatgtatca tgccagaacc ttttgccata cctccaatag
     4981 ttattttttt atctccatac attattgcta ctgctatttc tttttttata atatcagttg
     5041 tcataatagc ttctcttgct ccaatatgac cttgtttact tagattattc actaattcat
     5101 ccatattttc ttctattaaa tctatattta atggctttcc tataacacct gtagatgcaa
     5161 ccaaaacatc actttcttta atatttaatt tatcactggt gaatttagcc attttataag
     5221 catttataag tccttctttt ccattaaagg tattagcatt agcactattt actatgatag
     5281 cttgtgcctt tccattttta atgtgttcct gtgtaacata aacaggattg ccctttactt
     5341 tattttgagt tactactgct gctgcatcac ataaaacatc tgaataaacc aaagctaaat
     5401 ctcttttttc tttatttttt ctcaagccac agtgaatccc tgaagcaaaa aatccttctg
     5461 aaactgtaac tccacccttt ataatctcca tatttatcct cctaattata tgagtttagt
     5521 attttttatt tctaaaatga tggtgcaata ttttttaaac ctgtattttc ttcaatacca
     5581 aatattatat tcatgttttg tatagcttga cctgctgctc ccttaatcat attatctata
     5641 gctgaacaga ttatcaatgt atctccattt tcatgtaatg atattacaca tttatttgag
     5701 aatctaacat tctttaaaga agctacctta tctaatggaa gaatttctat aaattcttta
     5761 aattcataat aatcagttaa tttcctatgt atatcattta tagaaatatt atcttctttt
     5821 gtacaatata tagttgataa gattccccta tttactggga ttaagtttgg tgtaaaaact
     5881 acacttactt tttctttgca ggattcactt aaattttgtt ctatttctgg agtatgtctg
     5941 tgtttcccta ttttatatgc agtgatattt tcattaacct ccgtaaagtg actactaatc
     6001 gataattctc taccagcacc tgtaagacct gatttagaat caattattat attattattt
     6061 tttatcaatt tagaacttag taatggcatc aatggtaaag aaatactagt tggataacat
     6121 ccaggatttg caattatatc tgcatctttt atatcttctt tgtatatttc acttaatcca
     6181 tatacagctt ttttatgtaa gattttatct ataaaagata ctccatacca tttactatat
     6241 acttcttcat cttttattct aaagtcagca cctaaatcta taactttctt tttcgattta
     6301 attgccttta ttacaaactt ttcactaact ccatgtggaa gggctgtaaa cacaacatca
     6361 cacatatcta taactttttc attttctata catatcatgt tatttttata tcctatactt
     6421 ggatatatat caactatatc ttttccaaca tacgaatttg aacctatcgc tgtcacttcg
     6481 actttatcat gattcattaa taatctaatc aactctgccc cgacatatcc agttgcacta
     6541 ataataccaa cttttatttt attcattttg ttccctccat ttttatattc aaatagtctt
     6601 aaaatcctat ctttccactt tactatatga taatcatttg ataatacgta gattaaaata
     6661 ctctaattaa aacaaaaaac cccttagcat tataaatact aagaggcgat tttccgcggt
     6721 accactctta ttggtaaaat tacaataatc attaaaactc aaattaaaaa gtatctacaa
     6781 aatgtacatt taaaattaaa aaagcctctt ggtcaaagac caagagacga atatacaccc
     6841 gcgttaccac tctaattgat aagataatct tacccactct aatctcttaa ggcgagattc
     6901 accaattacc atactattac ttctggtaat ctcctccaaa gctctattca caataaaatt
     6961 cgttactagg cttccaccat tcccagctct ctatcaacta gctttatgct actctctttt
     7021 tcacaggatt tcaatttatt ttcttattgt tttatatgat agtttatttt tttaactttg
     7081 tcaatataat ttttaaattt ttggaaatat tttctatatg gttaatttcc agcattttgt
     7141 atctaaattg cgatataatt tttatatttc attcgtcaat ttaaaaaaca tccaaaatat
     7201 attaaattat aaaataatat attttcaaaa tacaatattt acatctcttt ttagttaaat
     7261 tttttaatgt tggtatctaa aaatttctca atctttttct cagaaagtat ctcgttggtt
     7321 acaaattcac cattaaaaaa aaagactgta tgtagtaaat ggtgcaggag cattttgtgc
     7381 ttcttttgct gtttcgaatt ttataagatt tacatcaata cctctagaat ttgcaatttt
     7441 tttcaaaata ggagcatatt tttccgtatg tggacactga ttactgtaat ataaaactat
     7501 accgttctca tttaattttc ttttttttac acaattctta aaatatggaa taggagcatt
     7561 atcagtaaaa ggaaaatata atagctcata atttggttct gatgtatctg ctaaacaaaa
     7621 gcccttatat tttagatatt tagcatcaga taaaaaaggc atttttttac tagacgaaag
     7681 cacaactaat ccatatttac cttttgattt tgcatcatca acacactctt gtagcaatag
     7741 attagaatat ccttgaccct taaattttcc agatatccaa aaacagttaa taaatatata
     7801 attgtccgca gtaataggac accatgcatt ttctgctgga atatattcta taaaagcttt
     7861 acctcttaca tcaaatcgtt taaagactag acctttatca aaattatttt tcatccaagc
     7921 ttttttagaa aaaacacaat tttctccttt tttttcagaa attgaacaac atatatgttc
     7981 attgtcaata ttatttatat ttatatcaat aatctgcata cttatctcct tttctaattg
     8041 cataataatt tttaaatata ttctatatat ttttatctta atattttgaa taacaacaac
     8101 atcatgtcat atatattttt attttacata atattatttt aagattatct gaattattaa
     8161 attgtatata taattaaata cattctatgg tctaactaca ctataatata aattatatat
     8221 tatcataata ttctaaattt ataaaacaaa ttgtattgaa ttgaatttat ttaagatata
     8281 taattataat taaaataata ataaaggtgt tgtttataat gttcaaggaa tacttaataa
     8341 gagaattaaa agaagatgat attaaaaatg cattagactt aatctggaac gtatttgaag
     8401 aatttgaatc tccagattat tgtgatgaag gaattacgac atttcatcaa tttatagaat
     8461 ataactctat tattgaaaaa atcaaagaaa aaactctctt cttttggggt tgttttgact
     8521 ctaaaaaact tattggagtc ttagctatta gaaatttaaa tcatatatgt atgttatttg
     8581 tagataaaaa ctatcataaa catggtgttg caaaaaaatt aatgactaca ggcatacaat
     8641 actgtatttg taataaattt aatttaactg aaataactgt aaattcatca ccttatgctg
     8701 ttgaagttta tcataaatta ggatttaaag atactgatgt tgagcaaatt gttgacggta
     8761 tacgttttac acctatgaaa ttgttaatat aataaattaa ataaaaagca aagtctctta
     8821 aatattttat agttactttg cttttaaacc aagatttaaa ataatactat cttttttgtt
     8881 ttttaataat atactttaaa atccttacta atatgaacta taatttattt tttgatatcc
     8941 tttttgatta acatataaat taattcaatt attaaacata aactatgttg tttctcttta
     9001 tatctcttct ttgtaaacaa tattgggtat ttgaaaataa atattcttaa aatatactgt
     9061 tctcctgttg attttccttt ggtacaaatg ataaaaattt aatttacatg ccccataatg
     9121 aagtgttcaa taaataagtt aaaataattt atttacctcc ttaatcatat tttttatgaa
     9181 tacctaataa cacagtatca atagaatcgg ctaaaccttt tatagcttct tctttactca
     9241 aaagtgattt cccaagttgc aaagtcgaat aaccatgaag tacactcatt attaagttta
     9301 atacttcacc tgtattcttt gtatttagat tacaagaaag aataagtttt tctaataagg
     9361 atttatattc atcaaagatt tctaatgttt cattatttcc atgccaagta gcccactgaa
     9421 ttgtttcata aacaccagga ttagaaatta taaaattaaa gtatgctata cttatacact
     9481 ttatagctgt atctccaaaa tttccaatag tcgcctttat catttgacta ttcatctctc
     9541 tcattccttt atgagctact tcaagtaata aatcatctag actttctata tgattgtaca
     9601 aagatggtgt acgtattcct agcttttctg caataacttt taaagaaact ttgttaagcc
     9661 catctttatt aactatatct gatgtagctt gtataactat ttctctacta acacgcatga
     9721 ttttccctct ttcatttaaa attaattact ttattagttt agctaatcta attagtttcg
     9781 tcaattattg taatttataa aattctttat actattataa aatcttgaca gtttaattat
     9841 aatttaatat aatactaatt gaattagttt taatactaat ataattagta ttatattaaa
     9901 ttctatatta caacattatt ttatagtttg attaatttaa caatcactaa tatatcaaac
     9961 tgagaggaga tttttatggg aaaaaaatat ttcaaaattc atggagttcc ttcaattctt
    10021 tggggaaatc cttcaagtaa attatatatt tacatacatg gacaaggtgg aaataaggag
    10081 gaggctgaag cttttgctaa tattgcagtt ccacatgagt gtcaagtttt aagtgttgat
    10141 ttgcctgaac atggagaacg taaaagagaa aaaaattcat ttaacccatg gcatgttgtt
    10201 ccagaattag tatctattat ggaatatgca aagtgccact ggaatcaaat ttcgttatat
    10261 gcaaatagca ttggagcatg gtttagtatg ttaagctttg aaaaagagaa cttaagagag
    10321 tgtatttttg tatctccaat tctagatatg caacagctca tttcaaatat gatgctctgg
    10381 gcaaatgtat cagaagaaca gcttaaacat gaactaatta tcccaacttc atttgggcat
    10441 actctttcat gggaatatct acaatatgta aaacaacatc ctatcactca atggaatgta
    10501 ccaacaaaaa tattatatgg taaacatgat gaacttactg aaatcagtgt tgtagagaaa
    10561 tttgtagaaa agtttaattg caaccttact attgtagaaa atggcaaaca ttggtttaac
    10621 acaccacaag aattaaattc attatatgaa tggttaaata ctttagttaa ataaaaaata
    10681 attatattat taatatgatt aaattattta taaatgttat ttaattaatt ttattttagc
    10741 attattaaaa taactacata aagaataaat tag
//
LOCUS       DAEMHW010000129        10728 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEMHW010000129 DAEMHW010000000
VERSION     DAEMHW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10728
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.129"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            214..1374
                     /locus_tag="KPK67_002684"
     CDS             214..1374
                     /locus_tag="KPK67_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG2459628.1"
                     /translation="MIETGFAYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1717..1878
                     /locus_tag="KPK67_002685"
     CDS             1717..1878
                     /locus_tag="KPK67_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459629.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2071..2700
                     /locus_tag="KPK67_002686"
     CDS             2071..2700
                     /locus_tag="KPK67_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBG2459630.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLTLKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2939..4198
                     /locus_tag="KPK67_002687"
     CDS             2939..4198
                     /locus_tag="KPK67_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG2459631.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLTYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4456..5451
                     /locus_tag="KPK67_002688"
     CDS             4456..5451
                     /locus_tag="KPK67_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBG2459632.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5697..6764
                     /locus_tag="KPK67_002689"
     CDS             5697..6764
                     /locus_tag="KPK67_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBG2459633.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYNKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7298..9208
                     /locus_tag="KPK67_002690"
     CDS             7298..9208
                     /locus_tag="KPK67_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG2459634.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNVGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9271..9894)
                     /gene="ytaF"
                     /locus_tag="KPK67_002691"
     CDS             complement(9271..9894)
                     /gene="ytaF"
                     /locus_tag="KPK67_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBG2459635.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10082..10726
                     /locus_tag="KPK67_002692"
     CDS             10082..10726
                     /locus_tag="KPK67_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2459636.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQRPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
BASE COUNT         4390 a         1059 c         1757 g         3522 t
ORIGIN      
        1 aattgttgaa aaagatttta aattcagatt attcatttct aaagtaatta atgtgcaaat
       61 ctttattaat atatgtcaat agttaggtat atatgtattt aattagcatg atatagataa
      121 aagtatttat tttaattgtc tattatctga aaaagtcttt acttagaaat cataaaaatc
      181 aaattaatta ggaaaagaaa gggtgtgtat aatgtgattg agactggatt tgcatattgt
      241 agtttcttgg ttgtattcgt aggtgtaatt ttttttatgg ttgataaatt gaaatggaag
      301 gtatttgatg ttattaatcc aatgctttta atatacttag gttcagctat acttggatca
      361 tttggtttat ttgcacagaa tgaagatgtt ggaacgtatc aaaacataat aacagtgaac
      421 ttccttccat taatgttagt gtttttgtta attcaatgtg atttaagaaa aatattaaag
      481 atggggccta aaatgttatt atcatttttc tgtgctacat ttacatttat gcttggattt
      541 gttatagcat tcataatttt taaaaattca atgggtgcag attcatggaa aattcttggg
      601 gcagcatctg gttcatggat aggtggttca tcaaatatga ttgcagcagc taatgctcta
      661 ggagttgcag aagaaggact tagttatgca atacttatgg gttctatagg atatacagta
      721 tggatgtctg tttgtctaat gtctgtgaaa tttggaccta aatttaacaa atggacaaag
      781 tgcgatacaa gttttataga tgaggttaca tcaaaacttg aaaaagaaaa taaagataat
      841 acggcaccta cgtttgtaga acttatgatt ttactttctg ttgggtttgg agttgcgtct
      901 tttgcacaaa ttgtatccaa gatacttcca acttcaggta tacttaatga aactatgtgg
      961 gtagtacttg ttgcatctac agtatcaata ttattgggta tgacaaaaat atcaaagctt
     1021 aaagggagta cactggtagg aaatgtgttt atgtatttac tccttgcagc tacaggtagt
     1081 aaagcaaatt tctttggatt gtcagaagca cctgtttaca taatgtttgg attggtagta
     1141 gtccttgtac atgctatttc atttataggg cttgctaaac tatttaaact tgatttattt
     1201 acttgtgaag taggaagtat agctaatata ggaggagtaa ctacagctcc aatgattgct
     1261 ggggttcaca atcctgtatt aataccagta ggtgtactta tggggcttct aggaaatatt
     1321 atagggactt attgtgctct tatggtaact caaatattac atatgttagc ttaataaaaa
     1381 tatggtataa aattaataat ttaaataaga aaagtcattt caatttaagc agaagctttt
     1441 actgaaatgg ctttttttat ttgacaagat aatctatttc aaaaagatgt tttgacatat
     1501 ttagtgtata atattgtaaa atttactaaa aacgagatat atttaatttt tatattgaat
     1561 cgaacgtctg tatgatgtat aataaatata cagaacgaat gtttgcagaa cgggtgtttt
     1621 ttgaatttat tttaataatt tatgaatata tatcagaatg cataaatata tttaaatata
     1681 aagattaaaa agaatttaaa ggagtgttga taacttatga taattccatc aagaagaata
     1741 caagatataa cattaacaaa tttaaaaaat ggtgaagtaa ctttaattga attggatgaa
     1801 atatacaaga agatgggatt tttatttgtt gttagtgaag gcaaattgaa aaagataaaa
     1861 aaggagaatc atcattagaa atgaatgaaa taataaatat tataatagtt tataaaacct
     1921 aacttatgta gaatataaat acttaagtta ggtttttttg tgataaagtt tataagtact
     1981 caattttata agataaataa tggtcttaag tgaaaagtaa ataagtttaa attttagtaa
     2041 gtattatatt gcataagagg tgatattatt atgaaaatag taacctacaa tattcataaa
     2101 ggtatggatt caaataatag attaactcta actaaaatgg ggttatattt aaaacaattg
     2161 gattgtgatg taatttgttt gcaagaagta ttatatcctc aatttttaac tttaaaagct
     2221 gttttaaata tggatggtgt ctttgctaca aatgtaaaaa aagtaaatat gatatatggt
     2281 atttgtacat ttacaacatc taaaatgtta aacagtaatc attttttctt aactagcaaa
     2341 aaagaacaac ggggagctct ttgtataact atagatgcat atggaagaat tataaatgta
     2401 ataaatactc acttgggact tgataggcag gagagagcaa aacaattaga cgaaataata
     2461 gactatagga acagattagt ggggatagtt attttatgtg gtgattttaa tgagaaaaat
     2521 gtctttttaa gtatgtttaa tgatatggct atttcactaa acaagtctta cttaccaaca
     2581 tttgaaaaat caaactctag aatagattac atttttgtta ataaaaatac tgaactaaaa
     2641 ggatacacag tagaaaaaat ttacctgtca gaccattatc ctgtaatagg ttacatttaa
     2701 atttcaatat aaatgaaatt agttgttata aaataaacaa ttttttgtta agaaatagta
     2761 aaagaaatat aataaaaaat atcaaaaata aaactaatta taagacataa atttacaata
     2821 aagttacatt tgattaaatt ccttgtttaa caaaagctat tcaaatataa tctaaggtag
     2881 ttttgaaatg ggaactctat tatgcaaatt taagctagtg ttaaaatgag gtgaagttat
     2941 gggcagaaga acaacaagag tttcaagatt aggtaaagaa aaaaaacgaa gtgttttaat
     3001 tgtattggca gctttagttc taataatggg tggatttact tattttttta acagtaaatt
     3061 tctgtacaat gggaaaatcg ctaaaaatgt atatatagaa ggagtaaatg tatctgatat
     3121 gactaaagca gaggcattaa aagctataac agataagtat actccagaag acttaaatct
     3181 tacttatgat gggaaaaaat atacaatatc accaaaagat attgatttaa aatatgatac
     3241 agaagatgtt gtaaaagatg cttatgaaag tacaaaaaaa gggtcctatt ttcaaaactt
     3301 aaaaaaatat attgatataa gagtgaataa ggcaaatatg aagataaaag ctgaatatga
     3361 tgaggcaaaa cttagttcca aagtgtcttc tatagcagat agtataaatg ttaagatgaa
     3421 aaatgcttct attagtgttg gaagtggtgg actaagttat acagactctg ttgtaggtag
     3481 agaatttgat ttggcagcaa ataaagagtc tatatataat atgataaaaa ataaagagca
     3541 taaaacttta gaacttaaag taaatcttca aaaaccagat ataacaactg aacaagtaaa
     3601 aactgtaaat tctgttatag gtcaatattc aactacttat tcacaagctg tagagggtag
     3661 gtcatataat gtaggattat cagcaagaaa aactagtgat gtattattaa tgccaggtga
     3721 agaattttca tacaataaac ttacaggacc tagtaataaa gcaaatggtt acaaagatgc
     3781 gcctgttata gtttatggta agctagaaca atcagcagga ggaggtgttt gtcaaacatc
     3841 ttcaacagtt tataatgcgg ctcttctttc gggtatggag ataactcaag ttacaaatca
     3901 ttcatctgca tctacttatg tgcctaaagg gagagatgct actgtgagcg atggaggatt
     3961 aaacttaaag tttaaaaatc catataagca tcctgtatat attaaaaatt atgcaggtgg
     4021 aggaagtgta tcatcagtaa tctatggtaa ttctggtgac aaaccaaata tatctataga
     4081 ggtaaaacaa actggtcaga ataaatattc tacatatcga atatttaaag attcaagtgg
     4141 aaaggtcata aaaaaagaac atatatcaaa tagttcttat aaagaattaa aaaaatagat
     4201 aattttttag agaaatattt gtaatataaa agggagtgtc tcaaaatgaa ctttttagtt
     4261 tagagacact ctttttatgt gaaatcaaat atattttcat catttcaaca atacaaaaga
     4321 agcgtatctc tattttgaga cagccttttt tgtaaaaatt aacactatta gttcttgttt
     4381 taatattaca atcattaata tatagaaaat attcctatat attggtataa ttgtaaatag
     4441 atttagttat ataaaatgta tgaggaggtg acatggttga attttatagg caatagatat
     4501 gaggtagtaa attctgatga cgttttagaa attaacaaaa tctataaagc tagagatgtt
     4561 ttttacagga aaaatgtgtt gataaaagtt ataaagcaca ataattatat ctgtgaagat
     4621 tttgtttcaa atttaattga tgaaagtact gctttagatg agataaactc tccttatata
     4681 ctaaaaataa tagatgttgg gattcactgt acagaagaaa caacattata ttatgttgta
     4741 agtgaagact ttaatggtat cggactagat gaattaattc taggtaatta tcttcattta
     4801 gaagcaattg taaacataat gatacaagtt ttaaaagctt tagaaatgat acataggaat
     4861 cattcatatc atggtagttt aaaatcgagt agtataatag tagatacaga atacaatata
     4921 aaaatttgtg attttggaat tacaaaagca aataatgggg ttaatacaag aagctatgga
     4981 aacaggagat atttatgccc acatcaacta tgtataaatt atactgataa agaaagtgat
     5041 ttttttgcaa caggtcttat tctatttgaa tctatattta aaaaattgcc ttttggagaa
     5101 tcaaattcag aagaaaaaat gctacaatct attgataagg gattagattg gaatagtata
     5161 aaagcaatta atggaaatac tgaacttata aatgttataa aaaggcttct tggaagaaat
     5221 aataagtata ccagagccaa tgatattatt attgatttaa gtaaagtaat gtatgaaaaa
     5281 gcatatatag aagttgaaga tgagaaagaa gaagagcaga aacaagacaa agttatacaa
     5341 gttaaaaaag aatataaaag taaaatgaga aaaaaattac ataaaaaact tgttgttgcc
     5401 ggtctagcta tagtcatgat ttcaatgatt attattagtt ccataatata aaaattgtat
     5461 attaagtatt ttaaaatcaa tgtctataaa ggatatacat agaaatttta gtgtagtctt
     5521 aaattatgaa aggaagtggt tattatgtta acaaaaaata atgaggtatt aaacgtctag
     5581 gagtatttta acaaatagcc acctgctttt acagtttaga ataaaaaagc tgtggctaaa
     5641 ggtcacagct ttttgctact ttttaaaggc aggagaatat taaaaaggag caaattatga
     5701 gcaatataaa aataatagca agtaataaat gttggattga agatatagca agaaaacaac
     5761 ttgaagatat ttcaaagtta aatggaattt taagagtagt aggattacct gacttacatg
     5821 caggtaaaat acctgtaggt ttagcagttg agactaaaaa tattatttat ccacatataa
     5881 ttggaaatga tataggatgc ggaatgactt tatttaaaac tggggttcta aaaaagaaat
     5941 ttaaaaaaga caaatggatt aaatcactta gtaagattaa agatttgtct gatatagaaa
     6001 taaaaaacac atataaagaa gaatgtccaa ttttaaattt aggaactata gggagtggga
     6061 atcattttgt agaaattcaa tgtataagtg aaatttataa taaagaacaa tttgaacaat
     6121 taaaattttc atctgatgat attatgatgt tagttcactg tgggtcaaga aattatggag
     6181 aagatatttt aaaaaaattt tatgataaag atggacttga agttgaaagt gagaaagcta
     6241 tagattatat taaaaaacat gataatgctt taatgtgggc agagcgtaat cgtgaagcta
     6301 tcagtaaaaa aattatgcag tcaataggta catctggaga tgttgaaaca atattctcta
     6361 taaatcataa ttttatagag aaaagggaag ataaatttat acatagaaaa ggtgctgttt
     6421 caagtgaaag aggagctgtg attataccag gttcaagagg cagtctatca tatatagtta
     6481 tgcctactga aaatacagag atatctttat attctctatc acatggagca ggtagaaaat
     6541 ggtctcgttc agtttgtaaa tctcgcctta aaagtaagta taataaagat actataaaac
     6601 aaactaaatt taaaagtcag attatatgta atgatgtaaa tttattattt caagaagctc
     6661 cagaggcata taagaatata gagcagatta ttgaaagttt gatagaatat gaacttattc
     6721 aagtagtggc aactatgaag ccattgatta cttataaagg ataattttta aagttaaaaa
     6781 agattaaaat tatattataa atagctaatt ttatagaaca tttaaaagtg ttttattatt
     6841 agctattttt ttattttgta gagacgataa tttatagagc taaattcttt gatatataat
     6901 gattacaaag ttagactgta ttggaaactg cacatacatg aattgatgac aatataagtg
     6961 attcccaaat ataaatgaaa gtataagctt attttcacta taaaaatttt cactgtacaa
     7021 aaacattaat gacattattt ttattgacat aaatatataa tttatggaat aatattagat
     7081 gcactgatat aagtgtttaa ataagaatag aaaaggagaa gtgttttatg aaaaaaaata
     7141 ctaacattaa tgagtccgga gttaaacttc ttaataaata aggagtttga tatataataa
     7201 ctccttattt attaagttgt tcgcaaaata actattatca aagtaaatga tagggtgatt
     7261 taaaagacga tgacttataa ataaaggaga aaaaataatg aatagtaata catttgaaag
     7321 attacaacta aatgaagtta aagacttaat aaaaatacat tgtgtaagtt cgctaggaaa
     7381 aaatttaata gataaattaa ctcctagtgg gaatattgga gtagtaagaa ggaaattagc
     7441 agaaaataaa gaagctagaa agataattga aaatagtaat cacataccac ttgaaggatt
     7501 gtttaatgta ggttctacaa tagataaaat tgaaaaagga atgataatag aacctgttga
     7561 acttgtaaat attgaagatt ttttaagagg ttgtaggaag atgaaagctt ttatgttaga
     7621 aaaagaattt tattcaccta cattaagttc ttatgcctta aatataacag aatgtaaaag
     7681 tatagaagat gagataaatt actgtataaa atctaataaa gttgattcta atgctagtaa
     7741 agaattaaaa aagataagac gaaatataga aattacagaa ggaaagataa aagatagatt
     7801 aaataaattt ataacatcaa ctgtaaataa aaagtatata caagagttta taataagtaa
     7861 aagaaatgat agatatgtaa taccaattaa atcttcttat aaaaatgaag ttaatggtac
     7921 tatattagat acatcatcaa aaggaaatac tgtatttatt gaacctatta gtgtttcaaa
     7981 tttaagtaca gagttaacta tgttaaaagc tgatgaaact attgaagaat acaaaatatt
     8041 atcttattta acagaactta tatttgagaa gataagtcag ataaaattaa atatagagat
     8101 actttcagaa tacgatatgg tatttgcaaa agctaagtat agtcaaaaaa taaaaggaat
     8161 tactccaaaa ataaataata atggctatat aaaaataatt aaaggtaaac atccacttct
     8221 aacaggagat gcagtacctt tagattttga aataggcaaa aattatagaa gtcttataat
     8281 aacaggtcct aatgcaggag gaaaaactgt aacccttaag actgttggtt tactaacttt
     8341 aatggttcaa tgtggatttg atatttctgc aaaagaaggt agtgaattta gtgtatttga
     8401 aaaagttttt gtagatatag gggataatca aagtattgaa aatgccttaa gtactttttc
     8461 atcacatata aaaaatattg cagagattat gagtttatca aataattcta ctcttgttct
     8521 gtttgatgaa ataggaagtg gaactgaacc taatgaagga gccggtttag caatatcatt
     8581 attagaagag ttttacaaaa tgggatgtat aactatagca tcaactcatt atggagagat
     8641 aaaaaagttt gctacattgc atccagaatt tgaaaatgct ggtatgatgt ttgataaaga
     8701 aacgttagaa ccattgtata agttaactat tggaaaatct gaagatagta atgctctttt
     8761 tatttctaaa aaaatgggaa taaaaaatag agttttagat agagctaaag aatatgtaat
     8821 tgatagaaac tacaatttag atttagttaa aagaagtaag ctacaatcta atattgaaga
     8881 taaaaatagt tctgaagtca ttttaaacta tacagattat aatgtagggg ataaggtaaa
     8941 attactagat gaagatgatt ttggaattgt atataagcct atggacaagt ttaataatgt
     9001 agaagtattg tttaatgata attttatttc agtaaatata aaaagattac aacttagcat
     9061 aaaagcatca gaattgtatc cagaagggta tgatttagat gtattattta ctagttttaa
     9121 agaaagaaaa ttagaaaaag atattcaaag aggttcaaaa aaagctctta aaaagattca
     9181 gaaagaaatt agagagaata gaaaataaat ataaaaagca caaaagaaag caatctaaag
     9241 agaaaagttt atctttagat tgctttctta ttaacatatc ttcataaatc caatgactaa
     9301 caagattaaa ccacttaacc aagataagtt taaatttacg tttttaacaa acatatttcc
     9361 tagtaaacaa ccaagtaata ctgcaattat atttgatatt aaagaaaata gaataacttg
     9421 tatataattc acacctacaa ggccactacc aaaaccgata gctaatccat ctaaacttaa
     9481 agcaagtgca agtgtaaatg cttcagcaga tttaagcact ttagatttac ttgtatctaa
     9541 gacatcaggt tcgatactta cattaaaatt taatttaagg tctaacatat taaaattata
     9601 attctttgag tgtatagatt ttttcttaaa ataagattta aaaaaacttt caattagtct
     9661 agcacttcct aataaaaata aaataaagaa acatattaac attgttatat tttgaggtaa
     9721 aaaatctcta actattgtac ctgcaaaaag cgatattgct aaaatagctg aacaaactac
     9781 gctgataatt atatttgaat ataagggaac tttaacttta tttacgccat atccaaagct
     9841 tgtcacaaag gcatctatac aaagagaagt aactaataaa attgattcaa tcacagtaga
     9901 ttaacatcct ttctcttaaa ttttgtactt atattagtta tagtattgta aaaatggata
     9961 aattgttaca gataatccaa taaattgttg ataaaattcc aacatcatac aacacctaaa
    10021 taacaaaaga atcatatcat ataataatat aaaaccaatc ataataaagc gggtgaaaat
    10081 aatggaacaa aataataagg ttgggaattt cggtgatttg ttgaagattt tattagatga
    10141 aaaagaatta tcaatgggag aactaagtaa aaaaagtgga attgataaat ctactatatc
    10201 tcgtatagct aataataaac aaaggccaaa tataaatcat ttagaaaaga tggcaataca
    10261 tttaaatatt aacctagaag aactgttgaa agcttcagga tatgaattta gaaacagcaa
    10321 taatcaaata tttaatgcag atagtgattt tagtaacttt gatgatattc ttggatttgc
    10381 aaatttaatc aatgataaga actttaatgg aaatattgaa aaagagttaa gtaagtgtaa
    10441 gttatatgtc caaacagatg aaggtaaaaa attacttttt gataatttta ataaaaaaat
    10501 agattctatt gaaaaacaag gtcaatttac agatagatta agacaaatgt atgctgattt
    10561 ttgtacagat ggacttacaa taaagaaata tttattgatt ggaagtatgc tactttactt
    10621 tgttatatca acagatgtga ttccagactt cgtgttccca ataggattta tggatgattt
    10681 agtagcatta aatatagtta caaaattact taaaaatgat aagtaagt
//
LOCUS       DAEMHW010000130        10583 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEMHW010000130 DAEMHW010000000
VERSION     DAEMHW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.130"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1113)
                     /locus_tag="KPK67_002693"
     CDS             complement(<1..1113)
                     /locus_tag="KPK67_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG2459637.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSF"
     gene            complement(1339..2577)
                     /gene="fabF"
                     /locus_tag="KPK67_002694"
     CDS             complement(1339..2577)
                     /gene="fabF"
                     /locus_tag="KPK67_002694"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBG2459638.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            complement(2606..2830)
                     /gene="acpP"
                     /locus_tag="KPK67_002695"
     CDS             complement(2606..2830)
                     /gene="acpP"
                     /locus_tag="KPK67_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG2459639.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            complement(2884..3633)
                     /gene="fabG"
                     /locus_tag="KPK67_002696"
     CDS             complement(2884..3633)
                     /gene="fabG"
                     /locus_tag="KPK67_002696"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBG2459640.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            complement(3633..4583)
                     /gene="fabD"
                     /locus_tag="KPK67_002697"
     CDS             complement(3633..4583)
                     /gene="fabD"
                     /locus_tag="KPK67_002697"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBG2459641.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAVLGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            complement(4599..5528)
                     /gene="fabK"
                     /locus_tag="KPK67_002698"
     CDS             complement(4599..5528)
                     /gene="fabK"
                     /locus_tag="KPK67_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBG2459642.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            complement(5637..6623)
                     /locus_tag="KPK67_002699"
     CDS             complement(5637..6623)
                     /locus_tag="KPK67_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBG2459643.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            complement(6649..7671)
                     /gene="plsX"
                     /locus_tag="KPK67_002700"
     CDS             complement(6649..7671)
                     /gene="plsX"
                     /locus_tag="KPK67_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBG2459644.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            complement(7682..8239)
                     /gene="fapR"
                     /locus_tag="KPK67_002701"
     CDS             complement(7682..8239)
                     /gene="fapR"
                     /locus_tag="KPK67_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBG2459645.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            complement(8446..8622)
                     /gene="rpmF"
                     /locus_tag="KPK67_002702"
     CDS             complement(8446..8622)
                     /gene="rpmF"
                     /locus_tag="KPK67_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBG2459646.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            complement(8647..9171)
                     /locus_tag="KPK67_002703"
     CDS             complement(8647..9171)
                     /locus_tag="KPK67_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBG2459647.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            complement(9264..10460)
                     /locus_tag="KPK67_002704"
     CDS             complement(9264..10460)
                     /locus_tag="KPK67_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBG2459648.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
BASE COUNT         3083 a         2148 c         1130 g         4222 t
ORIGIN      
        1 gaaactagtc tctgaatctc ttatactttt gttctcttta tattcattta ttaacatctt
       61 ttgatactca tttaaagatt ctatatttcc ttgtttttca tatatattta atatcttatt
      121 taaatttttt attttctcct gggttaactt tcttttggta gatatctcta aacttttttt
      181 ataattatct attgcagaat tataattttc gcttttatat tctaagtttc ccattgccat
      241 ataatagtac atatctttat ctaatatata aactgttcta tctgatttta taagataatc
      301 agctttttct aaaaaagtct ttgccttttg tatattattt ttgcccacat acgcatttga
      361 ctgcattata gaggccatta tatcaaaatc attgtaatta tccgtattta caaacttttt
      421 ataatctttt atcctgctac ttatttcaat agctttatca tattctccct cttctacata
      481 tatttctgct agatttataa tagcatataa tctaaccatt ccattttttt caccttcatc
      541 aatatctatt ttaaaagaat cctttattaa tttctctcct gtttcatagc cacctatctt
      601 taaaaatatg ctcgccaaat caacatctag tcttgctttt tcataattat tccccaattt
      661 ctcagccaac tttatagact ttacagttaa atctactgcc ttagaataat tattatccat
      721 tatatacact gatgctaatc ttttttctat attaaatcta cattcatcag ataatttcac
      781 atctgattct ctcatctttt ctaaagaatc aatgactatc ttcgttccat ctggaacata
      841 tgaaatagtc tttaacatat cccaaacttc attatatact ttattgtatt ttttttctgg
      901 aatagattca aaagaagtct taaccacttt ttttgcttca tcatactcct tatctgccac
      961 aaggctttca gacatatatt ttcccttatt tataatttct ctttctttca atccattttg
     1021 gtcatacttt ataaacaaaa taatactaat aagtattata catataaaaa ttatcttttt
     1081 gggtaacctt ttctttggta gtctaaaatt caaaatttta gtcctccttt tgtatattgt
     1141 agaatgaaaa cttgcatata taaataacct cttaataatt aataatacaa tatatcaaaa
     1201 tgatttacaa catatcctag acttaaatct taataaaaaa gtctcctaaa ataatttagg
     1261 agactttaaa tttaagctaa tataaattta tacagttaaa acaagtgctt gttttttagt
     1321 tttatattta atatttattt atacatattt ttttagtact attgtagcat tatgcccacc
     1381 aaatcctaaa gaatttgata gtgcatatct aatatttttt tctttacctt gatttggaac
     1441 ataatctaaa tccaaatctt caccaacttc ttgtatgttt atagttggag gcacaaaact
     1501 atcttctata ctcatagcac aaacaacagc ctctatagca cctgatgctc ctaataagtg
     1561 accagtcata gattttgttg atgacacaca taagtcatat gccttttctc caaatacagt
     1621 ttttatagcc atagtttcat ataaatcatt gtaataagtt gaagttccat gagcatttat
     1681 ataatctact tcttctaatg gaacatttcc atctttcaaa gccatattca tagacctagc
     1741 tgctccctct ccattttcag ctggagttgt catatggtat gcatcagcag ttaaaccata
     1801 tccaactact tctgcatata ttttagcgcc tcttgctaaa gcatgttcta agtcttctaa
     1861 gataacaata cctgctcctt ctcccattac aaaaccatct ctgtctttat caaatggtct
     1921 tgatgcagtt ttagggtcat catttctagt tgacatagcc ttcatgttac agaatccagc
     1981 aaaagcaaga cctgttacag cagcttcaga accccctgct accataatgt ctgcatcacc
     2041 tctttgaatt accttaaaag catcacctat tgaatgagtt cctgatgcac aagctgtaca
     2101 tacattagtg ttaggccctt ttgctcctaa aaatattgaa acttgaccag ctgccatatt
     2161 tcctatcatc ataggtacaa aaagtgaact tactctttta tttccttttt ccaaaagaat
     2221 tttatgttga gtttctatag cttcaactcc acctattcca gaacctatta taacaccaaa
     2281 acgttctcta tcaactttat ctaaatcaag atttgcatct tcaactgcta atttagcaga
     2341 tactattgca aattgagcaa atttatctaa tctcttcaat tcttttttac ttatatattc
     2401 ttctggatga aaattcttaa cttctcctgc tatctgagtt tgatatgtgc tagcatcaaa
     2461 attagttatt ttatctatac cagatactcc agattttata ttactccaga actcttcttt
     2521 tcctgttccc aacggcgtaa cacatccaag tcctgttatt actactcttt tattcatcgg
     2581 atacctccac ttttttaaag ctcgactatt tattagcttc tatatactta actatatctc
     2641 ctatagattt gaagttttct gcttctgtat ctggtatttc tataccaaat tcatcttcaa
     2701 gtgccattat tatttctact gcatctaatg aatctgcttc taaatccttc attaatgatg
     2761 tctccatagt tatttcttta tcttctactc ctaattgctc tattattatt tctacaactt
     2821 tattaaacat tgtgtacctc ctaaaatttt ttattttaat agatttctct actttttaaa
     2881 ttattattgc attaccattc caccatctac atttattact tgtcctgtta tataacttga
     2941 catgtcacta actaaaaatg ctacggcatt ggctacatct tctggtttac cataagattt
     3001 ctttggtatg tttgctagtg ttgattcttt tacatcatca cttagtactt tagtcatatc
     3061 tgtttctata aatccaggtg ctactgcatt tatatttatg tttcttgatg ctaactctct
     3121 tgcagaagct tttgtaaatc caattacacc tgcttttgat gcacaataat tagtttgccc
     3181 tgcattaccc ataattccaa ctactgaagt catatttatt attcttccat acttcttttt
     3241 catcataggt ttagtaactg cttttgtaca attaaagaca ccttttaagt ttatatctat
     3301 aactctatca aaatcttctt ctttcattct cataagcaga ccatctttag ttatgcctgc
     3361 attattaacc aatatatcaa tttttccaaa ttcgcttaca acagaatcta tcatttgatt
     3421 cacttcatca aatttagaca catcacattt tatagaggta cactttaccc ccatactttc
     3481 tatttcattt ttagttttta gtgcttcatc ttctttagaa gtataattga ttactacatc
     3541 agctccaaaa gatgctagtt tttttgctat ttcttttcct atacccctag agccaccagt
     3601 aacaactgct acttgtcctg taagatttat cattatatct ctccatttct ataattttgt
     3661 acaaattcat taagagaatc tataccatct atattataaa tattgacatt tgctttttta
     3721 ttggaagcta tcttcttaat aaatgctttt aaagtttttc caggtcccat ttctataaaa
     3781 gtatcatatc catcatttaa aagtctttct atagaatctt cccaaagaac agatgaactt
     3841 acttgtttta taagcaggtc tttaccttcg tttttatagt aatctgcatt tacatttgcc
     3901 accacatcaa ctttaggttt agttatgtta actttttcta attcactggc caattttaca
     3961 ccagcttctt ttaacattga tgagtgaaaa ggtccactta catttaattt aactgccctt
     4021 tttgctcctg cttctttgca ttttaccatt gcttcttcta tcttttcatt ttcaccagat
     4081 ataactattt gtccaggaga attgtaattt gctacttcta caatacctcc ttgtatatct
     4141 ttaaccacat tttcaagtac atttctatct aaacctaaaa ctgctgccat agctccaaca
     4201 cctgctggaa ctgtttcctg cataaactta cctctttttt taactaaaca tacagcatcc
     4261 ttaaagtcaa ttgcatctgc tgcaaccaac gcactatact ctcctaagga taatcctaag
     4321 catgcattat attttaaatc tatttgggct tgtaccgctt ttaatactgc tacactatgt
     4381 gtaaccatag caggttgagt gttttcagtt ttagaaagtt cttcttcatt tccattaaac
     4441 ataaggtttt ttaaatccat atttaaggca tcactagcct catctatcac acttttagca
     4501 acagcattat tttcatataa atccttggcc atacctacat attgagctcc ttgaccaggg
     4561 aatactagtg caacttttcc catcttgctc ctcctgattt acagttctat tgctggcata
     4621 atctctcttg cttgatttac catagcctct ataatttcct tacaaggtgt tatttctttt
     4681 accatagcag cactttgacc tgccataaat gaaccttttt ctatgtctcc atctactgtt
     4741 gcaaatctta aagctcctgt acctttttta tccaattctt caggagtagc tccattttgt
     4801 tccatcttta aaaattcttt tgatagttta ttttttaatg ttcttactgg atgaccagta
     4861 cttcttcctg ttacaattgc atctctatct tttgctttta gtactaagtt tttatagttt
     4921 gaatggacag aacactcttc actgcaaata aatctagttc ctacttgaac tgcactggca
     4981 cctaatgcaa atgatgctgc aattcctcta ccatctacaa ttcctccagc agctattaca
     5041 ggtatgttta cagcatcagc aacttgtgga actaagacca tagtagtaag ttctcctata
     5101 tgtcctccac cttcagtacc ttctgctatt actgctgtag ctcctagctt ttccattctt
     5161 tgtgccaaag ctattgtagg tactacagga ataacctttg ttccagcttc ctttaatcta
     5221 tccatatact ttgcaggatt tccagcacca gtagtaataa cttgaacttt ttcttctata
     5281 attaaatcaa ttatatcatc aacaaatggc gacataagca ttacatttac tccaaaaggt
     5341 ttatctgtta attttttaca ctcaacaatt tctttcttta tagcttcttt tggtgcgttt
     5401 cctgctgcta ttatgccaag tcctcctgca ttagatacag cacttgctaa tgatgcagta
     5461 gctacccatg ccattccccc ttggataact gggtatttta tatttaatat tttgcaaatt
     5521 ttattcatac aattctcctt cacttgaaat cataaaagta aactttttat tattaatcaa
     5581 ctttattttg tgtataagtt taattttgtt aattaaatca ttttagatat tttatactat
     5641 aaagtccact tgacaactga agccccccaa gttagacctc caccaaagcc aactaaaact
     5701 acattatcac cctttttaat tttaccttct ctataagctt catctaatgc tactggtata
     5761 gatgcagcag acatatttcc atatttgtct atatttacat aaactttttt catatcaagc
     5821 tctaatctct tagaagaagc ttgtatgatt cttatattag cttgatgtgg cactaagtag
     5881 tctatatcag ttgtatttag atttgctttt tcaagagctc ttaaagaagt ttctgccatc
     5941 tttctaactg caaatttaaa cacatcacta cctgccattt ctattgtatg tagattagct
     6001 tctacagtct ctttacttgc cttaagtctt gaacctccag caggtacttt taaacaatct
     6061 tttcctttac catcagagcc catatgtgta gacagtattc caccctcatt tacagggcca
     6121 ataacagctg ctccagctcc atctccaaac aatactgctg ttgttctatc atcataattt
     6181 aaaaacttag aacaagtttc tgctcctatt acaagcacat ttttatagca acctgtttcc
     6241 acaaattgct ttgcaatagt tactccatat acaaatccag aacaagctgc tgatatatca
     6301 aatgcagtag cattcactgc tcctattgca tcttgtacta tacatgcagt tgaaggcaaa
     6361 gacatatcag gagtaattgt agctaatata atcagctcta tatcctctac gtttaacttt
     6421 gcacattcta tagcttttaa ggcagcttta gatgctaagt cagaagttgc ttcagactct
     6481 ttggcaaatc ttctttcctt aataccagtt ctagtactta tccactcatc tgatgtatcc
     6541 attatttttt cgaagtgaaa attatcatat gattgctcag gtagataact tcctactcct
     6601 aatattccag cctttgtatt cattatatat taaacctcca aaacgagttt attcatcttc
     6661 gttattttta ttttcttcat tgtatttgct tataaattgg ttaatatctt caacgacttt
     6721 tccctttgaa aattttattc cttgatttat agcatttttt atagctttag catcagaact
     6781 accatgagct tttataactc ctccatttag acctaaaagt ggagctcccc catactcaga
     6841 ataatctata aaacttttta attttcttaa atcatccttt ataagtaatg ctcctatttt
     6901 actttttgta ctggccataa aagtttcctt tataagactc ataactgaaa gtgctacacc
     6961 ttcagcagat ttaagtaata tattacctgt aaatccatca catatgatta tatctgtgta
     7021 tgcatttata acttctctag cttctacatt ccctataaaa tttaaatcta attttttaat
     7081 ttcttcatat gaccttttga ctaaatcatt gcctttgcct tcttctagtc caacatttgc
     7141 taatgctacc ttaggatttt caagacctaa aacttttctt gaataaatat tactcatagc
     7201 tgcaaattca actagattct taggtttaca atctgcattt gcgccacagt ctgctataag
     7261 tgtcattcct cgctttacat ttggtattgc tgaacataag caaggtctat cgattccttt
     7321 tattctacca acaacaaata atccaccagc aagtaaggct cctgtatttc cagctgatat
     7381 tatagcatct gcctttcctt cttttacaag atttaatgct actaccatag atgaatcttt
     7441 tttacttctt atagccttta ctggtttatc ttcgttttct ataatctcag tagtatgaac
     7501 tatctctaat ttatttctat caaattcata atttgaaaat tccttttcaa gtaaatcttt
     7561 atctcctgtg attattaaat caacctgata ttcttttata gcatttactg cgccttctac
     7621 attagattta ggtgcattat ctccacccat tccatcaatt actattttca taatttccct
     7681 cctaatctaa ttcttcaaag ataaactttc ctctaaatat ttgctcttgg gctttattgt
     7741 tgataacaac ttttacataa tgctttttat ctatatttct atacacttct gcttttgcta
     7801 taagtctatc ttgttgttct atcaacttaa aactttttac atttgcaact ctcataataa
     7861 ccttaggtgc atctattata gccattgcca atgaatctgc tagagaaaaa atatacgtat
     7921 cttttaaagt attagtctta gagtaaatca tgtcctgtgt aacttctaac atagatatac
     7981 ctaatcgccc tacagataaa tcaatgattt ctcctgtaat ttcctttact cctaaagtct
     8041 ttacttttga actctgagtt tctgctattg atttgactct ttctcttagc tctggtatgt
     8101 ttagactcat tctatctaat cttatggtct gtatacttac atcaaacaga cttgataatt
     8161 cttcatcagt ataaaaagga tctgtcttta gcatatctat aagctctttt tgtctttggg
     8221 ctttactttt ctttttcatg ttccctccaa attttatagc tgtaattagt accagctact
     8281 aatagtagta tatagtaaca tacaaaaatt ttcaatactt atattattat ttttctattt
     8341 tttaatatgc ataaaatcca cttattttct ctagtaaatc atcttatttt tttatatgta
     8401 caaaaaaaac atgtagagta tactctacat gctttttaga agctattact tagacacaac
     8461 ttctttacct ttataatatc cacagtctgg acacactcta tgtggtaatt ttggctcatg
     8521 acattgtggg caacttacaa atcctgttgc ttccatcttt gagttagctg ctcttctcat
     8581 ttttgtgttt gatttagacg ttttacgctt tggtactgcc atttacgcca cctccttagt
     8641 caaaatttaa ttaaataaat cttttaattt agcaaaacga ggatctatgt attcctcatc
     8701 gtttgtaatt tcactgcaag aacactcttc ttcatttaag tttgccccgc atacttgaca
     8761 aagaccttta cagttttcac tacaaagaac tttatgaggt acattaaaat ctaatgtctg
     8821 ttctattata tcaataaggt cgatttcttc accatcgaat ataaaaggat caaattcctc
     8881 aaactcatct tcatcataat cttcttcttt tactaaaaaa ccttgtatag aatactctag
     8941 tggtatttct acttctttaa gacatcttga acaattgtca acaattgtaa aattgacatc
     9001 tatatctaga tacaaacctt tattagtctt tgacactttc ccttttaaat ttacaggtga
     9061 tactaatttg taaatctcat cacaataact aatagtatca attttttcac aaaaattcaa
     9121 gtctattttg tcagtttctt ttctatttat tttttctatg ctaactttca ttaagttcac
     9181 ctcaacatat caccaaattt attatacaaa tctagcattt gtttgtcaag tatttttact
     9241 tgataggcat aggtttaatt taattatttt accaatctta atgtatctct agctatcatt
     9301 aattcttcat tagttggtat taaaagcatt tttactttag aatcagcagt agatattact
     9361 gtttcttttc ctctaacatc attagcttct ttatctaatt tcattcctaa aaactccatg
     9421 ttagaagcaa tatctgctct tatagccttt ccattttcac caacgcctgc agtaaatact
     9481 acaacatcca atccattcat ttcagcagca taagcgccta tatatttttg tactttttta
     9541 acatacgctt ctaatgtagc ttgagctctt tcgtctccat ttttagctgc atcttctata
     9601 tctctgaagt cactagaaat tccagtcatt ccatatactc cagattcttt attcattaat
     9661 ttatccaatc catcagcatc taaaccttct tttctcatta agaatggtaa tatagctggg
     9721 tctatatctc cacatctagt tcccattatt aatccttcta atggagtgaa tcccatagat
     9781 gtatctacac attttccacc atcaacagca gctatagaag caccatttcc taagtgacaa
     9841 gttactattt ttaaatcttt tatatctttt cctaatatct ctgctgctct ttgagataca
     9901 taattatgag aagttccgtg gaatccatat cttcttaccc catatttagt atatagctca
     9961 tgaggtaaac catataagta tgaagattta ggcattgttt gatggaaagc agtatcaaat
    10021 acacctacca ttggtacatc tggaagtata gctttacaag catctattcc cattatgttt
    10081 gctggattat gaagtggagc taaatctata cattctttta aagcttcttc tacttctgga
    10141 gttattacta ctgaagttgc aaatttttct ccagcatgta caactctatg tcctactgca
    10201 tctatttctt tcatttcttt aactgcacca aattctggat gagcaacagc ttctaaaact
    10261 aatgctattg catctttgtg gtccttcatt ggttgttcaa caacgtattt atcatctcta
    10321 cctgcttttt catgttttaa tatagaacct tctattccta ttctttcaac caatcctata
    10381 cataaaactt cttcattatt catatctatt aattgatatt ttaatgaaga actcccacag
    10441 tttaatacta atattttcat tattatttcc tccttagatt tttttataaa taatatgtaa
    10501 taaattttga tttgtaataa atgtctaaat aaaactctat cttttattat tttgttatag
    10561 atatattatt gtatacatta aac
//
LOCUS       DAEMHW010000131        10538 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEMHW010000131 DAEMHW010000000
VERSION     DAEMHW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.131"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(258..2012)
                     /locus_tag="KPK67_002705"
     CDS             complement(258..2012)
                     /locus_tag="KPK67_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBG2459649.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKAIRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            complement(2230..3120)
                     /locus_tag="KPK67_002706"
     CDS             complement(2230..3120)
                     /locus_tag="KPK67_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2459650.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDIAR
                     QVLRLLEEHNLIPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAA"
     gene            3269..4129
                     /locus_tag="KPK67_002707"
     CDS             3269..4129
                     /locus_tag="KPK67_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2459651.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            4565..5131
                     /locus_tag="KPK67_002708"
     CDS             4565..5131
                     /locus_tag="KPK67_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2459652.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            5171..5752
                     /locus_tag="KPK67_002709"
     CDS             5171..5752
                     /locus_tag="KPK67_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG2459653.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(5809..6726)
                     /locus_tag="KPK67_002710"
     CDS             complement(5809..6726)
                     /locus_tag="KPK67_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2459654.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIEVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            6856..8487
                     /locus_tag="KPK67_002711"
     CDS             6856..8487
                     /locus_tag="KPK67_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2459655.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNTGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            8490..9437
                     /locus_tag="KPK67_002712"
     CDS             8490..9437
                     /locus_tag="KPK67_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBG2459656.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(9722..9985)
                     /locus_tag="KPK67_002713"
     CDS             complement(9722..9985)
                     /locus_tag="KPK67_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBG2459657.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(10084..10329)
                     /locus_tag="KPK67_002714"
     CDS             complement(10084..10329)
                     /locus_tag="KPK67_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459658.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         3688 a         1497 c         1435 g         3918 t
ORIGIN      
        1 attgagtctt taacattaaa ttaattgtaa ttttcatttt ttgttgttat atttaatttc
       61 aattttatct actatgaatt gataaatact ctaatttatt tataatataa aaataaggct
      121 atgattttta caaccatagc cttatcgata aatacttaat tatcacttaa aaaataattt
      181 tttatttaaa gaatatatat aaatatttta aaattctata atttttaaaa ctatataaac
      241 tttattttca tatgttttta ttctccaatt gatatattat ttatgccatt atttttaact
      301 tcaataattg gttgtccatt tcctaaaata taatctttag accaaccttc tggcaattta
      361 tttatcccat ttccaattaa cttcaagtgt aaatttgatg gttctttttt cagatttaat
      421 ttaaatattc catcaaaggt atctttatta tcgcttccaa ataaacttat attgccattt
      481 atatttgtag ctgttacatt tacaattcca ttgtttgctc taaattttat ttcagatgat
      541 atagtttcag ctgttacacc tgaaatccca ctattagtat ttatattgaa aggatttgtt
      601 acattgttat caacaatgtt agcaattccg tcattgacat caacagtagc tcctccttta
      661 atattataaa gttctactga accactttca acatctactt taaaattaga atacatttta
      721 tcgggaactc caatttttat cacatttacc atcctatctt tatttgcatt tatcatattg
      781 tcgccattca tatcttgcaa atcactatct ttcttatcta tttttttctt agcacttaca
      841 actagctttc cattttttat attattagaa gaattttctt tatatacaga aaaagtacaa
      901 tttttttcat tagttacact tacgtaatca aactcaaatt tattggtatt cgtcttttca
      961 acaaccacac ctaaattatc tgattctata agtatttcag aaacttcatt tccttcaact
     1021 gaagttaagt gttttaaaat atttggagtc atatccatat tattatattt aatcatatct
     1081 aaacactgtt tatatatatt ggcatcaata cctccaactt ttgttatact tttagctcct
     1141 tttaaaatac ctttattact tccatcagca actaaaacga ttggtttttc tttggctaaa
     1201 ggcgataatg ttagtgcatc tactaattga tatccatttg ttatataaaa atctgtagct
     1261 ccattataaa acttttccat taccttttta ttggtctcaa atctatcatt tcctccgatt
     1321 ctaattgctt tagtttcatt tactaaatcc tcatttatcg cagatttgcc acctatagcg
     1381 taaacattta aaccttcagt cttaaatgga actgatttcc catctgtaag tattatagct
     1441 cctttatttt ttgcaactac aggtgctata ctcatagcat cagcctctcc tacaaatcca
     1501 tttgtaagca ttacctcttt tacactactt acttcattta tttttttagc tacattataa
     1561 ctagtttcaa ttctattgtt accatttatt ctttccactt gaataccttt tctttttata
     1621 tctttttcta ctgaattttc aatagtatta taaccaccaa ttatatatac tttttttaca
     1681 acatttaaac tcttattatc tagtctattt aaagtagctt ttggtatttc acttttttta
     1741 gtcaaaagta ttggtgcgtt aatagctcca gctaatccac ttgatgaaag cccatcagat
     1801 aaatttttat ctccattaac taatattaca gtatcataag tttgtctatc cgctataagt
     1861 cctgctgttt catatctatc aattcctatt attgaatctt ctttaatctg tgcatttgca
     1921 gttaaattat taaaaagaaa catagtcaaa actaaaatta atatacttag ttttttactg
     1981 aattttttga attttttatt cataatatac actattcctc ctgtaaatta aggtctttaa
     2041 tttgcttata aattatcccc ttatttacaa ataataacat aatattatag taacgtatat
     2101 attaccttaa aattatcagt aactttatat caatcatctt agcttattta tataaatttt
     2161 tttatattac ttttaaaata acagtcatac taaaacaatt tcatcatata atttgaaaat
     2221 tattactatt tacgcagctt ttatcatcat cttaccacca tcaaatatat attcaaccat
     2281 attattctca tataaataag ttatataaga acgtattgta cttccaacca acacatattg
     2341 attagcatca agcttgacat tgtacaaatc acacattttt tcaataactt catcaatcat
     2401 aactggctca taacaaacct ttttaatatt atctattatt tcttccatct tcttcttatt
     2461 aatattaatt aaacttttta tatctgtagt tttgacagag tgacttggaa caaagaaatc
     2521 agcctctaac attctcaatt tttctagtgt cttaaactgt gaatctatat ccagtaggaa
     2581 gaaaaaatga tatttagtaa ttatattctc tggtgtaaga ctatctgcaa caaacaaaac
     2641 attatctgga gttctaacac caatcatttc aaaataatga cctggaagag aaatcgcttc
     2701 aagttctgta tctacaattt tgccatttga agggataata taatcaactt cactttcctt
     2761 tgctaataaa aatttattct tcagtgcttt acttggatag ccaccatata aaaatgctga
     2821 ttctaatatt ggattttctg taaaatatcc ttcaatttta gtcgtagcaa tcttgcattt
     2881 tgtttgattc ttcaaatatg catttcctcc tatatggtct gcatttgaat gagtgtttat
     2941 tattaattta ggaattaaat tatgttcttc taataatctt aaaacttgtc tagctatatc
     3001 cttattattt ccactgtcaa ttaaaattgc atctttacca tctacgtata cacctactat
     3061 tgtagggagt ggaatataat atgtatttcc ttttatatgt tctagagtta atttagccat
     3121 tttataacct ccctttaaat ttacttttaa ttatagcatt aattttctga aaattccaat
     3181 caaattgttt ttataatata tttttatttc ttttattaaa aattggaaaa actatataat
     3241 atatattata atctatatat aaggagtgat gttattgaaa aaagatagaa taactttttc
     3301 agaaatttta atattaattg taggctgtat attaatggcc atttctctta acttattttt
     3361 taatccacat gcaatcgctg ctggtggtat aacaggactt ggagttgttt taaattcatt
     3421 attcggtgta gaactttgga ttgtcaattt acttttaaat gttccattat tcatatttgc
     3481 ttacaaaata ctttcaaaaa aagattgttt taaaacggtt ttaggaataa tttttcttac
     3541 catagcactt aagcttactg caaatatggc tacattagat ataacaaatg atatgtatct
     3601 tgcaattata tctggttcta ttttaatggg agttggtcaa ggacttattt ttagaataaa
     3661 tggctctaca ggtggaactg atttaatggc actcctacta aataaatatt ttcctacatt
     3721 tagtatacct gttttaatgg gtattgtaga ctgcgttgta gttgttttat caggtatagt
     3781 aaatagacag gttgaaattg cactttattc aactgtggct ttatacattt tagtaaaagt
     3841 tagtgacttg cttattgaag gatttaatta ttccaaatca ttcactatta tatctgacct
     3901 ttcaaaagat ataagtaaaa aaataatgga agatttagat agaggtgcaa ctattctaaa
     3961 aggagaaggt gcatatactg gagaaaataa aaatgtactt ttagtagtag ttgaaaagaa
     4021 agaagttgtc gaattaaaaa aattagtaaa aaatgtcgac cctaatgctt ttattataat
     4081 tacagatata catgaagctt taggtaatgg atttaaaaaa atagaataac taacaaataa
     4141 atagcaagta taattaacat atacaaattc aactttttat atgttatatt atacttgcta
     4201 tttatatatt ataattgttg tttttaagat aaaaattaaa tgtatcaatt attgttttat
     4261 acaagtattt ccaaatccgt tctcatttag atagtatgct tagaaatttt agaattattg
     4321 agtctaactt tattacttac ttcactttat aacaatcaaa tacaaattta aatattataa
     4381 aactacaaaa taatttaaag catataatgt taatatgttt aaattataaa atattcatgt
     4441 agttatataa taaataattt ataaatatag ccaacttagc ctaaatacgt atataatata
     4501 gctaatatgg ttcttatact tgataccatc aataaaacta aaaatataaa aaggagaact
     4561 taaaatgagt gttagtttac aaaaaggaca aagagttagc ttagtaaaag ataataatcc
     4621 tgtaaaaaat ctagtagtag gcttaggatg ggatatgaat aaattgggta aaaaaaatta
     4681 tgacctagat gcatttgcaa tagcattgac taaccaagat aaaatgaatg ttagaggtga
     4741 cttggtttat tttggaaatc ttaaacaccc ttctaaagct attatacaca ctggagataa
     4801 cttaactgga aaaggtgaag gtgatgatga acaaataatt gttaatttag aagacatacc
     4861 tgaatatgta cataaaatag tttttggagt ttctatatac aaagctaaaa aaagagacca
     4921 ggattttggt caaatgaaca actcttttat aagattaatt gattcaaatt ccaagcaaga
     4981 actatttaaa tataatcttc aagaagactt ttctggtaaa gtaacagttt tggcaggaga
     5041 aatttatcgt agaaatgaag aatggaaatt taatgcttta ggaattggtc aaaatgaaga
     5101 gcttcgtgaa ttaattaata catataagta ataattacaa taaaaaattt ataatgagga
     5161 gttggtttaa atgggtataa ctttagcaaa aggacaaaaa gtaagtttaa caaaaagtaa
     5221 ccctggtcta aaaaaagtaa tagtaggact aggatgggat attaataaat atgatggtgg
     5281 ctttgatttc gatttagatg cctctgcatt tttaactgga actgatggaa aagtaacgaa
     5341 tgatggagac tttatattct ataataattt aaaacacgct tctggagctg ttgaatatat
     5401 gggtgataat agaacaggtg ttggtgatgg tgatgatgaa caaataaatg tagacttatc
     5461 taaaatacct caaaatattg ctaaaatatc atttagtgtt actataaatg aagcaattac
     5521 aagaagacaa aatttcggac aagttgaaaa ttcatatata agaatatata atgaagaaac
     5581 taatgaggaa cttattaaat atgaattggg tgaagatttt agcatagaaa cagcaatagt
     5641 tgtggcagaa ctatatagac ataatggaga gtggaaattt aatgctttag gttctggatt
     5701 tgaggatgga cttgcaggat tgtgtaaaaa ttttggcgtt aatataggct aatatgtcta
     5761 aataaaaata agagtatatt gatatactct tatttttatt tagacatatt ataaactgtt
     5821 tacacttcta ctcttctttc tcgcttgttt tttcccatat tctactgaaa tatcaaaaaa
     5881 tgctgaaata ttttctgtaa aaaacttgct tctatgatat gcaatatcaa atgtcctatg
     5941 taaattaata ccttcaatat cacaagccca caaaagatgc ttttttaaat aatcttcaat
     6001 aagtaattcg gatataactg caatgccaaa attatctaca actgctctta atatagcttc
     6061 agagttataa catgaccatc taatattcat tgggattttt aattctttta attgcttttc
     6121 taattgtgct cttgtaccac ttcctaattc acgcataatt aatggttgat ttgaaagttc
     6181 tgaaacttct atagagtctc ttttataaaa ctcatgcttg tgtgaacata taagtacaag
     6241 attatcatta ataatgcttt ttgtaacaat atctgggtgc ttaatttttc cttctacaat
     6301 cgcaatatct aattcactat taagtaattt ttttgcaatc agtcttgtat cttctacaaa
     6361 tacttcaggt tctataagag gatttacctt gtataattct aacattatcg ggcttattac
     6421 acatgtacca acagttactg ttgccccaac tcttatacgg atattttttg cacaatgctt
     6481 catacaatca ttcatttctt catttacagc taaaagatgc ctcccatatc caagaagttt
     6541 tttccctgcc tcagttaaat ataatttttt tgaaattcta tcaaatagct taacaccata
     6601 ctctttttct atttctgaaa tagcttggct aactgatgac tgggtaatat acatatttct
     6661 tgctacttca ctcattttac cacattcagc aaccttaaca aagatttcaa gactacgaat
     6721 tgtcatatta tcacaacctt agcaaacatt agtaaatgct tatgatttta ttaatatttt
     6781 agtattttac aaatatattg tcaagaagta taattttatt atagagttat aaagtagatt
     6841 ggagtgtgat atattgtgaa agtattaatt ataggtggag ttgctgctgg aacaaaaact
     6901 gctgcaaaaa taaaacgtga aatgggtgat aattgctctg ttacaatact aaataaaggt
     6961 gatgatattt cttatgcagg ttgtggtctt ccatattacg ttggtaagat aattgaagat
     7021 aaatcatctt taatcgtaaa tacacctgaa agtttttcta aactaacagg agcagaagtt
     7081 cgttgtggag ttgaagttac atctattgat agaaatgcaa aacaagtaac tttgaaaacg
     7141 ctttcaacag gagaaaaaga aaatctaagc tatgacaaac ttgtaattgc tacaggggca
     7201 gacccaataa aaccaccaat acctggaata gatttacctg gagtattctt tatgcgtact
     7261 ccaaatgatg ctataaaact aagagattct attgaaaacg gtattaagag agctgttgtt
     7321 attggtggag gtttcattgg tcttgaaatt gctgaaaatc tttctgcaat gggtatacgt
     7381 gtaagtgttg ttgatatggc tgaacatgtt atgcctggat ttgatactga ttttgctgaa
     7441 tacgttgaaa atcatatggc tgataaaggc ataatgattt ttacaggaga ccaagttgtg
     7501 ggtattgaag gtgaaaataa agttgaaaaa cttcgtacta aaaatagagc tattaaaaca
     7561 gatttagtag ttatgtcagt tggaatccgt cctaacacag gttttctaaa agacacaggt
     7621 ttagaatttg ctcctaataa tacaattatt gcagatgaat atatgcttac aaatgacaaa
     7681 gatatatacg tagttggaga ctgtgctttt gtaaaaaatt ctttaacaaa taaacctaca
     7741 tggtcaccta tggggtcatc tgcaaatcat gagggtagaa tttgcgcaca aaatatatct
     7801 ggaaaatcta aaacttacaa tggtgttctt ggaacaacag tagtaaaatt acctgaactc
     7861 aatacaggaa aaacaggtct aagtaaagaa atagctgaaa aagaaggtta taatgtttgc
     7921 tctgtaacca tagctactga tgataaagct cattattatc caggtgcatc taattttatt
     7981 ataagaatgg ttgctgaaaa aaatactaga aaattattag gtgttcaagt tatgggacct
     8041 ggagcggttg ataaaatagt agatatcgct gctactgcaa taactttagg cgctgacctt
     8101 tattcattag aaagtatgga ccttgcatat gcaccacctt tttcaacagc tattcaccct
     8161 tttgtagtgg cagtaaatgt tttacaaaat aaacttaatg gagaacttga tagtgtttta
     8221 cttgacgaaa ttgaggattt tgacagttgg actaaacttg atgtttcaaa agctccttct
     8281 attcctagtt tacgttatat tcctgttggt gaaattaatg gagaaattga aggccttgct
     8341 aaggatgaaa aaattcttct tatatgtgct aaaggtaaaa atagctatat ggcgcaaaat
     8401 cgcttaagac gctttggcta tacaaatacg aaagttcttg aaggtggaat actattttat
     8461 ccaaatctta aggtaatgga ggagtaaata tgagtaatgt tacagcagaa caaaaaaagg
     8521 aagtaaaagg tagaggtttt ctaccaagca gggatggaga acattttgca gcacgtataa
     8581 taactgtaaa tggtgtaata aatgcatcac aagcaaaaaa aattgcagaa gctgctgaaa
     8641 aattcggaaa cggtcagtta gcatttacta ctagactaac agtagaatta cctggtgtaa
     8701 aatttgaaga tattgaagct ctaagtgaat ttattgctag cgaaaatcta ataacaggtg
     8761 gaactggatc aagggttcgt cctgttgtgg cttgtaaagg tactgtttgt gttcatggtc
     8821 tagctgatac acaggcactt gctgctgaaa tacacgaaga attttacaaa aattggtatg
     8881 atgtaaaact acctcataag ttcaaaattg gtgttggtgg atgccctaat aactgtatta
     8941 aacctgacct aaacgatctt ggaattgttg gtcaacgtgt acctgactat gattcagagc
     9001 tttgtgttgg ttgtaaaaaa tgtgctgttg tagaggtctg tcctgttaaa gcagctaaat
     9061 taacagataa aggtaaactt gaaatagata gtaatctatg taataactgt ggtaagtgta
     9121 tagaaagttg taattttgat agtattgaag aaaaagaatc tggttataaa gtctatatag
     9181 gaggaaaatg ggggaaatca gttcgtcctg gaacacagat tgatagatta ttttcaaaag
     9241 aggaacttat gactttaatt gaaaaagcaa ttcttttatt tagagaacag ggtaacacag
     9301 gtgaacgttt tggaataact atcgatagaa tcggtgctga taaatttata gaaatgcttt
     9361 tatcagatga agttttggaa agaaaagaag agatactaga ggctctttta caccttacag
     9421 gtggagctgt atgctaacaa taaatatttt aattgtacag ataacctatg gaaaaaattt
     9481 tagttaagaa taattaaaat tctatatatg atatatgata aatgaggcta tctcaaaaca
     9541 cagatacgcc tctttttcat tgttgaaatg ataaagtata tttaagttta tacaaaaaag
     9601 agtgtctcgt gaattataaa attcattttg aggcacccac attattttat aataattgct
     9661 tattaatttt attttaacta tataaaatgt acaaaaatta agaatatcca atattctaaa
     9721 attaattttg aattgtattg attgaaaagt ttactattcc actatttcca tcttgattta
     9781 ctactagata ccatgttcca gtattaggaa ctctcattct acatggattt ttctttgcaa
     9841 atccaccata gtaatcataa tttctttgat ttttaaattt cacataattt atatggtcta
     9901 acaatattac attggcttgt ttctctaaat ttacctctac aatatcccct ttttttaagt
     9961 tcatcttact ataactatat tccattgaat tactcctcca gtatcgaata atctttgtta
    10021 tttactcatc tcaaatatgt aaaaattcat ttaaaatatt acaaatacta tataattaga
    10081 ctattatcca gttttaacct tatttactac aatccaaagg ttattaaaaa tattattggc
    10141 aacaacttct ccatcccata caactctttc ttctttattt tctaataaag catagtaccc
    10201 agagtcagaa ctttcatctt tatagactgt aacataaaac tcttcatcac tatattcagg
    10261 tatattttta aagtcttcag atattttaca tgttaatata taaccttcta attcttcatc
    10321 tataaacaaa gcaattcctc caatctctat atttttacta attataccat attatgtcat
    10381 aaacttttta ttttattaca aatttctatt ttttctttac tttataaata aattatataa
    10441 attttcactt tttaacttta tattatacta attaattctt ttgttttcct atatatttct
    10501 ttataattta taaatttagc cattaattta tacgattt
//
LOCUS       DAEMHW010000132        10465 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEMHW010000132 DAEMHW010000000
VERSION     DAEMHW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.132"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            274..1155
                     /locus_tag="KPK67_002715"
     CDS             274..1155
                     /locus_tag="KPK67_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2459659.1"
                     /translation="MPAYLFDSNSITNIYRVFSILLSFIVTGLYFSYKNHKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            1203..1513
                     /locus_tag="KPK67_002716"
                     /pseudo
     CDS             1203..1513
                     /locus_tag="KPK67_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(1773..4340)
                     /locus_tag="KPK67_002717"
     CDS             complement(1773..4340)
                     /locus_tag="KPK67_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG2459660.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKESLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(4343..4948)
                     /locus_tag="KPK67_002718"
     CDS             complement(4343..4948)
                     /locus_tag="KPK67_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2459661.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTNTALVT
                     RLLINSMLSISYTSRPSDSLEKMLDETMNIFLHGIMNDGGE"
     gene            complement(5322..6782)
                     /locus_tag="KPK67_002719"
     CDS             complement(5322..6782)
                     /locus_tag="KPK67_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG2459662.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            7106..7684
                     /locus_tag="KPK67_002720"
     CDS             7106..7684
                     /locus_tag="KPK67_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2459663.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPQKEGFAKALKMLYRLYDKN
                     PFLYSPNNTDYITLLNKIPKEMLSQLEYSNVQSFYSLIERHNLKLKIEKEKAFGVCNA
                     LLSSLLLKEDIGYDYIEVFDFMVDNLVEHILE"
     gene            7735..7968
                     /locus_tag="KPK67_002721"
     CDS             7735..7968
                     /locus_tag="KPK67_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459664.1"
                     /translation="MIELIQRLNDIIYKINDKLNIHTKLVDPSSELMPLYSMLSIIVS
                     IFLIILGIKVRKKFLIIIAIISIIVNVLIILDR"
     gene            7977..9227
                     /locus_tag="KPK67_002722"
     CDS             7977..9227
                     /locus_tag="KPK67_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2459665.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            9388..9957
                     /locus_tag="KPK67_002723"
     CDS             9388..9957
                     /locus_tag="KPK67_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG2459666.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3500 a         1779 c         1515 g         3671 t
ORIGIN      
        1 aaatatctag acataatgat taataactct aaaaataaat acaaagtata tattgtatac
       61 tttcttttta attatttaat ttttattatt tgtacttatt taaggttaca tctagttata
      121 aattggacaa tttttatgtt aattttattt atgcaaatta aagtaactta caaactttcc
      181 acaataaaaa acctttttta ttcgttaaac tgctccatca ttggtttagc aataaatata
      241 ttttttaggt ctttaatgtc aatagtgtta aatatgccag catatttatt tgatagtaat
      301 tctattacaa atatttacag agtattttct atattgctta gttttatagt tacaggatta
      361 tatttcagtt ataaaaatca taaaaataat aagattaact ttgaggcagt tttagaggac
      421 aaaaaaaact taattttctt attgagcctt atgtttggaa tgtacttgta tctatgtttg
      481 agtttgtcaa tatactatac tgttggaaat gtattgattt taaaattata tggaatcaag
      541 tctgccataa tagttttggt aggtaactat attgcagaaa gatatagctt tagaattact
      601 caaataaaag gttatgatat taaaaatcaa atagaatatg tagaaagtgt aaagacagag
      661 tatgaaatta ataaattgaa tgatattgta tatacagatt tactgacagg cttctacaat
      721 agaccttttg taaatcaaaa actatcagag tggacatcaa atcactacaa atttacctta
      781 tgttttactg atttaaatag cttaaaacct gtaaatgata aatttggaca ccaatttggt
      841 gataaataca tatccactac tgctcaaata ataaaaaaac ttataaatgt agaaaattcc
      901 ttactgttta gatatggtgg agatgaattt ttattccttt tacagaatac aagttgtgta
      961 gaagcagaaa aaataatgat taatataaat tatgaattat caaaattaag taatactgac
     1021 gagtatcctt atgctctttc cataagctat ggattagttg agtgggatgg aatatccgat
     1081 attgaaactt taatatcaaa ggcagattct ttgatgtatg aaaataagct taaaaataaa
     1141 tctactttaa actagctagt taagatattc tacaaaatat atgttataag agagtactaa
     1201 gaatgtcaaa atatatagat tttgcaaata ttgaatacag tttactaatg aatgcactgg
     1261 atgtaagtgt tagtaaacac ctattagatg aacactttac tgttgtttgg gctaatgacc
     1321 gttattatga aatgttcggc tatacctagc taaaaagtta aaaatgacca ctgtagtcga
     1381 gggaatagag accagttttc aaaaagattt cttgcaagaa ataaactgtg atatgttaca
     1441 aggatacgtg ttctctagac ctttaccaat aaaagacttt gaaaaactta tgtttcaaaa
     1501 ctcctccaat taacaatata aaattgctta ttataagtga tattattgta tcaacaaata
     1561 aaggaattgt ctcaaaattg attttttcta atcaaatttt tattttgaga cattctcatt
     1621 tttttgcata agaaaatacc caccaagcta cggtttttct taccgctgtt cggtgggtat
     1681 tagtagtatt atgaaatctg aaaactttaa gtattactac ttccgctagc gcaatcacaa
     1741 aggtcgaaag cttcaaagcg aaaataggct ttttactcat caaacgtcac cgtacctgcc
     1801 aacccgtcca ccgttatcat ttgtccgctc ttaatagttc gtgtggcaat gtgtgtagcc
     1861 ataacggctg ggatgccata ttcacgtgcg acaatgcttg aatggctaag aggaccacca
     1921 atgtccgtta caatcgcact tgcagatgaa aataatggtg tccatgctgg agttgtggta
     1981 actgcaacca aaacacttcc aggttgaaag ctttcaaaat ctgctggact attcagtaca
     2041 caagctggag ccgtcacaac accagtactg gtaccaatgc ccgtcaacac aattttccca
     2101 tctttctgct tctgttgtgc ttgtgatata gtttttttgt ttttctctgg caacttggat
     2161 ggtgacacat aacccatgta ttttttaagt tcagcttttc gaacaggtat tatcccgttc
     2221 atatcagata gagacttgtt tttatccaac tgctctataa gtacctccaa ttccgatttt
     2281 gtgagccaat aaatatcatc taaatgtgaa atagctcctc ctcgtatgaa tcgttctgag
     2341 atttcctgaa acatacaacg aatcagagga tgtcccattc ccatcaaata aatagcattt
     2401 tcacgcatag gagaggtttc ctgtgcccaa tggagcaact taaaaaatag ttttttacgc
     2461 aatccaccta tgtgttgtaa aatctcttct tctgcatgtt ttctacgctt ttcaaacgtg
     2521 gtctggcgta aaaaaggact ttcccctttt ccttccacaa atgtttttac ggattcaaaa
     2581 gttggtgtaa gtgtttcctg cggtgtggaa tgcgcaaaat caaactcata agcagtacga
     2641 ccaaactctt taaagtactt attgattcga tttttccatt ctatccatac ttcctgtgaa
     2701 actccaacag gcacaactga agacatgaaa tcttctgcta tctttattgc aggactgttt
     2761 tgcagataaa ggttgatgct gttattttgc tttacccatt ctgaaatacc ccacaactcc
     2821 ttttccgatt gcagaacaat cgtatcaaac ccaagcagga aaacggaagt atccgttata
     2881 ccagcacgtc gaactgctcc ctgaaataat tttgtaaata atgtttcact gatagaagca
     2941 gcaggcaatg taagctgaat ccttgtgaaa taaatacatg cggcataaaa tattgtctga
     3001 ataccctcca taatttgatg ggcgttcata gtatgcaggg gcttttcttc ccattgttta
     3061 atagcatcct catatccttt tcgtgccgtt tcccagcgtg caacgctgtt cataagtgtc
     3121 ctacgtaaag agcgtgggga aagggatttg atagcaatca aaagtggctt tgatttagca
     3181 gaaagataaa catatccatt aataacagta tatgccccgt tttttggtaa taattttttc
     3241 gcacttttac caaacatatc tatccataaa agctctgatg cacggtttac gatttcaaga
     3301 ccaagcgtgg cgaacaaagg tgtaacagga tttggcaagt gttctgccaa actttccttt
     3361 gtgtatacaa catcctgttc tggcaatacc cattctggtg gcagaacagt aattggacga
     3421 gcctgaacaa tgtacaattt atctttttcc agtgcccact ctacatccat aggcatttga
     3481 taatacttct caattttctt tccaagctgt atcagttgca ttacctgatt acgagtaaga
     3541 gcatattttt ttctcaacct ttcaggaacc atggtttctt ctgttccttc tgaagtacga
     3601 accgtcataa tctctttatt tgccacttca tacgatataa ttcgttcgga atccttatcc
     3661 actacgattg tatcgggtgt aactaagcta gaaaccaccg cttcaccaag cccccatgca
     3721 gcgttaataa tcatttcgct acgtctacca ttgattgggt ttaacgtaaa cataatacca
     3781 gatgaatcgg aaaatgcaag cttttgtact acaacagcaa gtgcgacaat ctcgtgcttt
     3841 atattattct tcacacgata agcaatagca cgagctgtcc ataaggaagc ccagcatctc
     3901 tttacagcgt caataacttt atcttcacct tgtatgttaa gataagtttc ctgctgacct
     3961 gcaaaagaag cgtcaggtaa atcttcagca gtcgcagacg aacggacagc caccgatatg
     4021 ttccctaatc cagcataagc tgttttaatt gcgtccgaca cttcctgtgg catttctcca
     4081 ttatggaaaa gctcccctat tttcttagat acatcttcaa gttggctggt attattggaa
     4141 tcaataccat ctagcagttt attaattcga gactgaatat ggttcgtctc aacaaaaatc
     4201 tggtatgaag cagtcgtaac atgaaaaccg tctggcacag ggatgcctgc tgtcaatagt
     4261 tttgataaag acatcccttt tccaccaaca atttcaatgg ttgcttgttt atgttccagt
     4321 ggcaagacat aataaatcat aatcattcac ctccatcatt cataattccg tgcagaaata
     4381 tattcattgt ttcatccaac attttttcca aactatccga tggtcgtgac gtgtagctga
     4441 ttgataacat ggagttaatt aacaaacgtg tcacaagagc cgtattcgtt ttacgaaaga
     4501 tgcctgtggt cacaccatta tcaattaccg attttataat atcttgatat gcataacgag
     4561 catctttaag tctctttcga tactcttctt ctaaataatc agattctgta tttaaccaca
     4621 tcagtacagt tctatactgc tctggaaatc taagatgatt aagcatgatt tttctaaggc
     4681 actgttgtgg tgaaggttca ccatcaataa tcctatgaac ttgttggata gttttttcta
     4741 tttctttctc aactgcatat acaacaatct cgtctttcgt tttgaaaaaa tcgtatagac
     4801 ttgactttcc catgttggct aaaactgcaa tttctctcat tgatgttttt tggaatccat
     4861 tttcagccat gagactcaaa gcaacagcaa ctattttttc tcggcgttct gcttgttgtt
     4921 cgggtgtaag cacaattcct tttggcatag ggcgatacct ccttttagcg accactggtc
     4981 gtttcttttt tatttatcat acagcgacca acggtcactt gtcaaggtta atttttttca
     5041 ttgtatatgg tactcgagat aaaaatgttc attttagaaa ttgctctatt taagtagaaa
     5101 taggcttcat cattagaaaa caagactata aatttgagat atttatcatg ttcaaatttc
     5161 atcaatatca aagtttaaga tattaataaa aaaagaatta ctaagaaata acatataaag
     5221 attcaaaaaa ggagttgaac ttaaaattca attacaaaaa atcaaattta agacaactcc
     5281 tttattcttt atctctacta ttctttatct ctatttattt attacaactc cctaacattt
     5341 accactactg ccttaggtct agtcatagcc tcgattgaat acttaacacc ttgagttccc
     5401 ataccagatg cctttacacc taaaaatggg aagtggtctg gacctctttc agtcttatta
     5461 tttatttgaa cagtaccaac ttcaagttta tcagctatat aaaatgcctt atcaatatca
     5521 cttgtaaata ctgatgattg aagcccatac tctgattgat tagcaatttc aattgcttga
     5581 tttatatctt taactcttat aattggaagt actggaccaa atggttcttc ccaagcaatt
     5641 ttcatatcaa catttacatt atctaacaat gttggataga ataaattatt ttttctaaca
     5701 ttgcctgtta ttaactttgc tcctttatgt aatgcatcat caaccaaccc ttgaacaaaa
     5761 tcagttgctt tattatctat aagaggagtg ataactactt catccattgg atttccaatt
     5821 gtcaacttct ctaccaatgg ctttattttt ccgactaatt tatccgccac actctcttgt
     5881 actaaaattc tctttactgc cgtacatctt tgtcctgaat atgaatacgc tccagatact
     5941 atattcttag ctgcaaaatc taagtctgca tcttctaata ctatagccgc atcttttcca
     6001 ccaagctcca ataataatgg taccattcca gatattttag atatatgctg accaacttca
     6061 gtactaccag taaagttaat aaaattaatt cctttatgcg ttacaacata atcccctatc
     6121 tcactacccc tacctgtaat agtattaagt actccatctg gtatcccagc ttttctcata
     6181 atttctacca catgaagtgc gctaatagcc ccttgtgtag caggttttaa tacgactgca
     6241 tttccaccaa taagtgctgg agcaatcttt gacattgata aatttacagg ataattaaaa
     6301 ggagatatag ctaaaacagt acctaatgga acccttgaaa cataagacat cttatttctt
     6361 gttccaccag ggaaattatc tccactaatg gcttctccct ccatattttt tcccacatca
     6421 gctgtgtatc ttaaaaaatc tgctgttctc tcaacttctg cccttgcaga ttttatgtct
     6481 ttggctattt caagtaccag tatattagct atctcatcta tattttctaa caataaatca
     6541 gcagccttat gaaatatatt ggctctctta tatacaggca tttcagccca tacttttata
     6601 ctctcttttg tgttttttat aacctcatct acctcatgtt ttgtcataga ttgtactttc
     6661 ccaacaagag aattatctat tggtgagtaa atttctattg gctttctcga attagattct
     6721 acccattttc catttattaa atttttatat acattttctt ttgttttaag ttcattaaac
     6781 atatttttat tcctctctct gatataactt tgtgtattta aattatagcc atttattcgt
     6841 tatgtaaaca tttctttttt tcataaacgt ttacctataa aaaaatttta agttaacgtt
     6901 tttctacatt cttctatttt aataacatac tcgctccaaa tctatatagg tcacacttta
     6961 ataccaatat accaatatac caatatacca atattataaa cataaatcac ttgacaaatt
     7021 tataagtaga caatattata tatctatgac ttaaaatatt tttaaagtca tagatatcat
     7081 atatagacag aaaggattga taaaaatggc taaaagtttt acagaaatag aaaaagacaa
     7141 tataagaaaa agactaattt cagaatgtga aatcaactgg tctaaatatg gatacaaaaa
     7201 aactaatatt gatgaacttt gttcaaaatc aggaattgca aagggttctt tctatacatt
     7261 ttttgattca aaagaaaagt tattttttga agtattaaat ggaatccaaa acagacttat
     7321 atcatcagta gatgaaattt taagtaacat gcctcaaaaa gaaggttttg caaaagcctt
     7381 aaagatgttg tatcgattat atgataaaaa cccattctta tattcaccaa ataatactga
     7441 ttacatcact ttattgaata agatacctaa agagatgctt tcacaacttg aatacagcaa
     7501 tgtacaaagt ttttatagct taattgaaag acataatctc aagttaaaaa tagagaaaga
     7561 aaaagccttt ggtgtttgca atgccctact gtcaagccta ttgctaaaag aagatattgg
     7621 atatgactac attgaagtat ttgattttat ggtagacaat cttgtagaac acatacttga
     7681 gtaataattt aaaataaatt ttctaaacat ttctttataa ctaggaggag taatatgatt
     7741 gaattaatac aaagactaaa tgatattatt tacaaaataa atgataaact aaatatccac
     7801 acaaaacttg tagaccctag cagtgagctc atgccacttt actctatgtt atctataatc
     7861 gtttctatat ttctaataat attaggtatt aaagtaagaa aaaaattcct tataataatt
     7921 gctattatat caattattgt aaatgtttta ataatcttag ataggtaggt tcatatatgg
     7981 taaaatcctc gaacactttt aataaaaact tcaacttgat gatattaggt caaataatct
     8041 cattgtttgg tgcatcaata cttaaatttg ctttatcttt atacatactt gatataactg
     8101 gtaaagcaga aatatttgct actatactag cagtatcttc aatcccaatt atagtattct
     8161 cccctattgg tggagctatt gcggatatat tcaacagaag aaacttaatg gttatttttg
     8221 atttttcaag tagtcttaca gtactaatct tagcagtatt cttattcaac aataatggtt
     8281 caatattact tgtaggaatt attatgacca tattatcagt cataagcaca atgtatcaac
     8341 caactgttca gtcaagtaca ccccttttag ttgataatga gcatttaatg aatgcaaatg
     8401 gtattgtagc tggagttgca tctcttacaa atattgcagg acctgtatta ggaggtgtct
     8461 tgtatggtgt gattggaatt aatgcaatta tagttatcag ttgtgtaagt ttctttctat
     8521 ctgcaattat ggagatattt atacagattc cttttatcaa acaagagcaa aacagcaaca
     8581 ttataaaaac tatatttact gatataaagg atggtattgt tcatatatgt aaagaaaata
     8641 gatttatact aaaaatactt tttttagcta tagcaataaa cttattttta agctctatgt
     8701 taatagttgg tatcccctat atagtaaaaa tcactatggg agctagtaac ttcttatatg
     8761 gtctagcaga aggagcaatt tcttttagtt cactggtagc tgctgttagt attggtattt
     8821 tctcaaaaca tctaaaaatc actaatttgc atatttcctt tattatatgt gcagttatgt
     8881 tcataccaat ggcggtttca gtatatccat ttatattaaa cacaggattt ccagttccat
     8941 ttttaatctt tatagtatca tctatgctaa tattttttac aacgactctt atctcaatat
     9001 atgttataac agtcacacaa ttaaaaactc caaatgaact cttaggaaaa gtaatgtcaa
     9061 ttctttttgc aggaacagca attgcaatgc cccttggtca ggttatgtat ggtaaacttt
     9121 ttgatttatt atcagataga gtgtacatca ttatactagg agttggattt attacattac
     9181 ttatagcatt tgtatcaaag ataacattga ggaacgaaaa tctttagata caaagaattt
     9241 agatataaag atttaatttt cactaatttc tgaatttaaa tgcaaaattg tgtatcagta
     9301 tgttgacgtt ttagaaattg attgatataa tcaatggaaa ataaacttta taaaaattca
     9361 aaatattcag ctagaggagt gataaaaatg agttacatgc caactttaga acaagcatgg
     9421 gaaatattaa gaaaatacaa taaggaagaa tttcatataa gacatgctca aattgtatct
     9481 ggagttatga gatactatgc aaaggagtac gaccctgaaa gagtagagtt ttgggaaata
     9541 gttggacttt tacatgattt agattttgaa atgtatcctg atgaacattg tgttaaacaa
     9601 agagaattaa tgacagagct tgatttagat aaatcaataa tcgattctac aataagtcat
     9661 ggatatggat taactggttc agatgttaaa ccagagcaat ttatggagaa agtattattt
     9721 gctgtggatg aacttactgg tttaattggt gctgctgcaa taatgcgtcc ttcaaagagc
     9781 gtttctgatt tagaattaaa atctgttaag aagaaattta aagataaatc atttgctgct
     9841 ggctgttctc gagacgttat aagaagtggt gcagaaatgc ttggatggga acttgataag
     9901 cttatccaaa caactataga agctatgaga agcttgattc cagagatggc aatatagctc
     9961 ctccatacat ataccataga agttacatct ttaaagagca tttctctttt tgatgcaact
    10021 tctttttata ttttgtagaa taaaaggctt aatattttaa actcactata taatatataa
    10081 taaatactat aaataatata atgtataata gtcataaact ttctaacctt aaaagtttct
    10141 aataatcaat aaacaattct aatatacaaa tgtcattata tgatttaatt atatatcttc
    10201 aatatatata ttaaaataaa tatatatata ttaaattctt atacaccaaa tttttacatt
    10261 aaacttctat gcaccacatt tatacatatt aaattcttat atattaaatt tctatgcatc
    10321 acttttatac atattaaatt cctatatatt aaacttttat gcatcacttt tatacatatt
    10381 aaattcctat atattaaact tttatgcatc acttttatac atattaaatt cttatatatt
    10441 aaacttttat gcatcacttt tatac
//
LOCUS       DAEMHW010000133        10463 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEMHW010000133 DAEMHW010000000
VERSION     DAEMHW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.133"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(56..403)
                     /locus_tag="KPK67_002724"
     CDS             complement(56..403)
                     /locus_tag="KPK67_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBG2459667.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(390..2318)
                     /locus_tag="KPK67_002725"
     CDS             complement(390..2318)
                     /locus_tag="KPK67_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBG2459668.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(2729..4918)
                     /locus_tag="KPK67_002726"
     CDS             complement(2729..4918)
                     /locus_tag="KPK67_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBG2459669.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRAFKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKFGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKKALPE"
     gene            5249..5980
                     /locus_tag="KPK67_002727"
     CDS             5249..5980
                     /locus_tag="KPK67_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG2459670.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFILRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LTLNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            6060..6695
                     /locus_tag="KPK67_002728"
     CDS             6060..6695
                     /locus_tag="KPK67_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG2459671.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(6946..7539)
                     /locus_tag="KPK67_002729"
     CDS             complement(6946..7539)
                     /locus_tag="KPK67_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBG2459672.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(7610..8473)
                     /locus_tag="KPK67_002730"
     CDS             complement(7610..8473)
                     /locus_tag="KPK67_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBG2459673.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGSDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            8636..9805
                     /locus_tag="KPK67_002731"
     CDS             8636..9805
                     /locus_tag="KPK67_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2459674.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLEKPNVKYT
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIVTA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            9906..10382
                     /locus_tag="KPK67_002732"
     CDS             9906..10382
                     /locus_tag="KPK67_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG2459675.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSIPKWCSHWPKSVEKSGEFNWEDEIEF"
BASE COUNT         3661 a         1717 c         1208 g         3877 t
ORIGIN      
        1 ataattacct tctaatttat atgctctaat atatctataa taattcttat tatatctata
       61 tatccagtct ttcaatatag tgacaattaa cttcaatatg atttaaaaat actaacaacc
      121 tttcataaag tggatgagat tcttctccat atttagcttc taggttcttg cctatttctc
      181 taacagtcct ttgaccatca atttgtaaaa aaacaaaact ggaatattca tctagttcta
      241 tttttttgta tttaggtatt ctagctttta actttctaaa aaacttttga attttatggt
      301 cctgtttttc aagtatagta acaacactat tttcagctac ttcatattca agtccatcag
      361 taattttaaa tacaatatct aaaacatcat tactcttgct cattattttt tactttagat
      421 aaaacaatag gtaatgctgt aagcacaact aaaactacta ataaagcaat tgccattgaa
      481 tttgttgcag caaatccact tggtaccttt ggagttacaa ctccagttac ttgtaatatt
      541 atacctatta aaccaatgat agaaccacca gcaacaagac cagaagataa acttatacca
      601 ttagaaactt taacttcttt ttctttttca gttttagata ctaattcaac aaataaacga
      661 attaatgctc caactaatat tatagaagtt gttgcaattg gtaaatagaa tcctatagct
      721 atagtcatta ttggtaaatt taatgcatat agaactattg ccataaatac tccaacaata
      781 atcataaccc aaggtaaact acctgacata ataccagaag ttaatgttga cattaagttt
      841 gcttgtggta atgcaaattg tacattatca ccagttgctc taagttggtc agaaagtaat
      901 aaaattactc ctacaacaac tataacacca actatacttg caattgtaaa gtaacgttgc
      961 atttcgtttt tgctaccacc aataatataa gttacttttt gagattgagt ataacctcca
     1021 gctatagcaa tagcaacaac tataaatgaa ccaaataaaa gtaatgattt attagcttct
     1081 aaatcagtcc atcccattat aacaaacact aatgttacaa taactagaga agctatagtc
     1141 ataccagaaa ctggaagatt tgatgttccg attgttcctg ttaaacgacc agctactatg
     1201 acaaataata aagataataa aagagatatg atagctccaa taattgccat aacaatgtta
     1261 ccagaaatta agaatgctgc taaaaatcca ataactactc cacctaataa aagtatcatt
     1321 tgtatagatg aaccttcttc tccttctcca gcacttgatt tagctttaag tgtttctttt
     1381 atagaagcga ttatagttgg tataagtttt attgcaccaa taatacctcc acaaagcatc
     1441 ataccagcac ctatatattt tacataacta ctagatattg ctccaacatc catttggtta
     1501 agaggcattg caggattatt ccataccata gctccatctt tagccatatc tgtaaagtat
     1561 cctattaaag gtgctatacc aaagtttgat aaaatacttc ctgcaaacat tgttaaagaa
     1621 acttcaagcc ctactataaa tccaattcct aaaagtagag gatttacttc aatatcaaat
     1681 ttccatttgt agaatttgct tcctacaaga ctcattacat tgtttgctac atttaagaat
     1741 gaaccagtta atacagtaat aaatccactt attacaaatc caattcccat gtacttaatt
     1801 gaatctccac cagcctctga agcaacaagt gtctcagata tagccattga ttctgggtac
     1861 ataagtttac catgttcttc aactattaga tagttgtgaa ctatagcagc aattcctata
     1921 ccaaataaaa ctccaccaac tccaacagct aagccttcta agaatgtaat ttggcttcca
     1981 attaatataa ctgctggtaa tacgaaaata aatccacttg caacagattc tccaccactt
     2041 gacatacctt ggattaagtt tttaccaaga ataccctttg aacgagcaaa tgctccaaca
     2101 aaagcagaac ctattatagc tcctggaata cctgcggcaa ctgtaagacc ggctttcatt
     2161 ccagagtaag ttgtagatgc ggcaaaaatg gcagctaaaa taataccgat aatcagtaca
     2221 gctacatttc caccggttct ggacttatct gtgatatatg gaacgtaatc ttttccacta
     2281 acctcaccat aagcaccctt aggtaatttt ttgttcataa aattttcacc ccttttcaat
     2341 ttgtatatat tataacatat atacatataa aacttaactt tttattattt tacaaatagc
     2401 gagtcaaaac tactaaaatt taaccctata ttattaattt ctacttttag atatttttat
     2461 gctaattttc tttttatttt tcaattctac taaaaactat taagatataa aataaaaaat
     2521 ttttttaata taaaattaat tttgtaaaaa aagtgcttta aaacacggaa aagtataaaa
     2581 aatacaataa tcattcttat aaataaattt tcaccatagt aaatatatac agaaaattct
     2641 tttttttaca tcaattgatt ttctcagaaa aataggtgtc ataaagaaat gtatggaatc
     2701 actttacaac acctgtcata ataaaaaatc actctggtag tgctttttta tttacttttc
     2761 ctattggtgt aactggtatt ttatctatcc tcattattat tgttggaatc atatatggag
     2821 gtagatgctc atttaaatat tctctaatag ttctttcatt tatcttacca ctatcttcct
     2881 catcaattac ataatatgca catatataca tatctccatc atcatttgtt ctcgatatac
     2941 aaacaacact tttaatttct gtaatattat ttattatatt ttcaatctca tatagctcta
     3001 tcctaaactc atcaattttt acttgattgt ccatccttcc aatataatct aaatttccat
     3061 catctaacca tcttactaaa tcaccagttc tatacattaa cttattatgt tcacaagttt
     3121 tgtaaggatt ctctacaaat acttgctctg ttagcccttt tttatttaaa tatcctcttg
     3181 atatttgaat gcctgataga catagctctc ctggagctcc aattggacat agtttgttct
     3241 ttttatctaa tacatatgca taagtattaa atataggttt tcctattggt atttttccat
     3301 actctctgtc cacatgaaaa gaagtcgata caacagtaaa ttcggttggt ccatactcat
     3361 tatatagttc ataattacgc tttctatatc cttttaatct ttctcctcca gtctgtacaa
     3421 ctctaagtga attattattg caattttgta taaataacct tgcaaatgct gttggcaatg
     3481 ctagtatagt tatgtcattt tcctcacagt attcatttac tgtatattca tcaaatttag
     3541 cttcttcagg cataatatct attgatgcac ctacaagcaa ataagggaat agttgtttca
     3601 ttacagcatc aaaactgaaa cttaagtata taccacatat atcattttct gtagcgctta
     3661 tagattttac gctataataa cacatattta ctaaactctt atgttctata gctatggtct
     3721 ttggcctacc agttgaccct gaagtagata tcgtatatgc acaatttgat gatgttattt
     3781 tttcagttaa ttcatatcca aacttaaaca tttcttcatc atttatatcg agtactatgg
     3841 tattctcatt tacaacctta cttttgagtt catctgtggt tatcactact tttgcattac
     3901 tatcttcaag aaggtaattc aatctttctt ctggattact tgtgtctatt ggaaaaaatg
     3961 ctccccctga tttcataatc cctattgctg taataattat gttaatatct cttggtaaca
     4021 ttacagctac tatgtcttca ctttccactc ctatatgatt taaataacct gcaagtttat
     4081 cagttacttc atccaattct ttatatgtta actttctatt ttcatcccta gcagctatat
     4141 tactaggtgt taccttaact tgctccctaa ataaatcaac ataactttta tcatagggta
     4201 cttcatattg attattatta aagtcttcca ataatacttt ttcttcttcc ttacatacaa
     4261 attctaaatc ttctattaaa atatcattat cttctattat ttgctctatt atataatcaa
     4321 attgatttaa aattcttcta atagtttctc ttttatatat agatggttta tactttattt
     4381 ttattttaaa tgccctctta gtatatacta ttacagataa atcatatcct tttttttcta
     4441 tgtcataatc atatgataaa tcaaaacttt tagctataaa tattgtgttt ataagcttag
     4501 tatttagatg atttttattc tctatatctg ataacaaata gtatccatac tctaaagacc
     4561 tgctccactg cctttttagc ttccttacaa catctataaa tttttcactt tgtctaactc
     4621 ttactcttat tggaattata ttcattgttt taccaaaaac tacatcatct tcatatatat
     4681 acttttgaag tataaggcca tatatagttt ctatgataga gtctatgcta atattgttca
     4741 ctttagaata ttgaattaac ctttcatgtt tctcaatatt catcttaaaa acctcatcta
     4801 ctacttcatc agaacaatct ttataaaaaa aatcatttgg aatttttgct atattcgtat
     4861 aatcatctaa caaatccatc caataattca tagctcgttc taaattaact tcatacatat
     4921 caaaattccc ccttactact aagtctatag aaaaaattct tttggaattt tattttcaca
     4981 atatataaat atattaataa cacatatgca agctctaaaa acatgagctt gcataaaatt
     5041 acacttatta tgactactaa attcgtaata tttacaatgt ttaaaatata ttataacaca
     5101 tatatgattt tatttttcta catttatata atatataaat tacaaatagt tatttaacta
     5161 atatataaca ataccttttg gaatatgata tattatatta attaaatttt tataagataa
     5221 gttatactta taggagggaa ccatataaat gaatatttat gctcataggg ggtttagtgg
     5281 aaagtatcca gaaaatacta ttctagcttt taaaaaatgt ttggatatgg atatatatgg
     5341 aatagaactt gatgtccaca gaactaagga tggaaaaatt gtagttattc atgatgaaaa
     5401 ggttgataga acttttaatg gacatggttt tgtaaaagac tttattttaa gaaaattaaa
     5461 aactctaaac tcatcttttg aaggatatca aagcaataaa gaatgtaaaa ttccaacttt
     5521 agaagaagta ttaattctaa tttcacctac agaccttact ttaaatatcg aactaaaaac
     5581 tgacaaaata aactatccca atatagaaaa agacgtttta gagcttattt taaagtataa
     5641 tatgaaaaat agagttctta tatctagttt caacagcaat agcttaaaaa actttcataa
     5701 attagaccct agtgtaaaaa caggattatt gtgttattta cctataaaca atgtagtcaa
     5761 ttttgctaaa tttctgggta atagttacct tcatccacct ctagttttag taaatgaatc
     5821 tctaattgaa ctttgtcaca aaaatctact tggtgtcaat gtatatactg taaatgaaga
     5881 ggatgatata ctacactgtc taaaactcaa tgtagatggt atttttacta actatccaga
     5941 tattgcttca aatcttttac actcaaaaca atacagctaa ttatacaata gtttgtactc
     6001 cttgactttt atataattaa tatgtacaat tttattaaat ttatcttgga ggtataacta
     6061 tggataactt attaaatgaa cttgaagctt taaagagtaa tttagataat ctaccaatag
     6121 aaaaaataga aaattatgat ttgagtaaaa ctgctttatt tataattgat gttaataatg
     6181 gctttgcaag acaaggtgca ttatactctc ctagagtaga gtctttaata aagccaattg
     6241 aaatgtttac aaaaaaaata tcaaacaaat taaatagagt aatagcattt actgattctc
     6301 atactcctaa atcaatagaa cttttgagtt atcctgttca ctgtttggaa aatgatgtag
     6361 aaagtgaatt ggttgatgaa cttaagtcga tagaaaacct acaaattctt cctaaaaatt
     6421 ctactaatgg tttttttgca ttagaaaatt tggattttga taatatagat aatataatta
     6481 tagttggcga ttgcacagat atatgtatat atcaatttgc aataactcta aaatcatatt
     6541 ttaatcaaca taatattgaa aaaaatatag ttgtacctat gaatttagta gatacttatg
     6601 atattcctaa cgttcaccct gctgagattt taaacttagt atttttcaac agtatgattc
     6661 aaaatggagt taatgtatta aaagagattc gttaagcctc taatacttta catcccatat
     6721 aatacaatta tcagatattt atttttaata aaatttctaa tagaaaaaca cagttttagt
     6781 gagaagatta caatttaaaa cactatctat tattactaat cttcacaata taaaaaatcg
     6841 cagtttagat attccttata caaatttata aggaataata aattgcgatt tttattttta
     6901 agtagtacaa ttaattgata ctacttcagt atttaaattc attttttaat aatcatactt
     6961 attcgtagtt tcgcacatta ctacctttat ttttttatta aacatattct tatattctaa
     7021 taaaaactca tttagttctg aatccataag atttattttt ctatcactac aaagcattat
     7081 cttttcttgg tcgattatta cattaatatc atttaggtca ttttttataa atgttgttat
     7141 acaactcttt ggttggattt tttctccata tttctcataa aatcctgcac tcttcttaaa
     7201 tgactctaaa tcttctgtag atactttgct ggaagtatta ttactcataa aacctgctgt
     7261 agaagggtcg attccaaagt ttctaagttg tttttctact tcttcagtgt ccataccata
     7321 tctctccata aattgctttt gtattttcat aaatttttct tgagatatgt tgttatctct
     7381 catataatcg aatatttttc ttgaaaactc agccatagac atctttccat ccataacaga
     7441 aaaatataag ttatatatgt attgctcaaa tttatctaca tcagaatctt taactttgtt
     7501 ctttagttcg ttaaagtcta ttattctatc atcagacatt tttcatcctc ctagctaaat
     7561 ttttacttgt ataagattat accatatttc tattatattt atatacacac tattttttta
     7621 atttaacgat tttttctgta gcatctaatt cgcacattct gcccaaagga atagttatca
     7681 ttgggataca atgtcctgac tgaagattat atatagaagg tttattgacc ttatttgcaa
     7741 attctattag taattccatc aaatcatttt cttcaataca atccttaaaa tctccaaaaa
     7801 ttataccatt acattcctca agttttcctg aagaatatag ttgtgttaac attctatcca
     7861 gtttatattg aggttctcca atttcctcaa taaacaaaat tttatctttt gcatctattt
     7921 catattttgt tcccattgtg gcaattatta aggataaatt tcctcccatt attattccct
     7981 ctgctttacc ttcacataca gtataaatct tttcttcttt tggattttct aaatataatt
     8041 catctttaaa ttctaaagca tttaccaatg atttataggt aaatttatcc catttaccta
     8101 catttgctgc cattattcca tgatatggta aaagaccaca gtttttatag aaagctaaat
     8161 ttaaacctgt tatatcagaa aaacctataa atggttttgg attttgcttt attatatcat
     8221 aatttatttt atccaatatt cttgttgttc cataacctcc tcttaagcac ataattatat
     8281 ctacatcttt atctaaaaac atatcttcta tatcccttgc tctaatatca tcactaccag
     8341 ctaaataacc tttataattt aaactacaac tttcagaaaa cttaacttca taaccataac
     8401 tttctaaatt aaactttatt tcctccaaac ttgtatttct taatggccca gatggcgcaa
     8461 ttattcctaa catttaaaca cctcttctta taaaatgata tattcttctc catactaaat
     8521 atctctaata taattataag atattttatt tgtacttgat acataataat gatttacata
     8581 aaataatata attaatgaag aaaaataaat ttttaatata gttaggatgt gttttatgct
     8641 taatataaaa actctttgtc atgagataaa tgattgggtt ataaatataa gaagagattt
     8701 acataagact ccagaacttg gattagaaga atttcaaact aagaaaaaaa ttattaaata
     8761 cttaaatgaa ataggtataa attatattga atataagaat catacaggga taactgcata
     8821 cattaacgtg agtccaaact ttgaaacagt tgctataaga gctgatatag atgccttacc
     8881 tataacagaa gaacttaatt attcttataa atctattaat attggcaaaa tgcatgcatg
     8941 tggtcatgat gcacacactg ctattttact gggtacttgt aatatactat ttaaattaaa
     9001 ggattactta aatgtaaatg taaaattctt ttttcaacct gccgaggaaa ctattggtgg
     9061 ggctcaactt atgatagaag atggatgtct tgaaaaacca aatgtaaaat atacatttgg
     9121 actccatgta aatcccaata tcaacaaaaa tctaattgaa ttaaaataca acactctaaa
     9181 tgcttccacc gatacacttc aattaactgt acatggttca aaatgtcatg gagcttatcc
     9241 acatcaaggt attgatgcta ttgttatatc tgctcatatt gttacagctc tacagacaat
     9301 cgttagtcga aacacaaatc caactgattc tgtggttatt tctctaggaa agattgaagg
     9361 tggaatcaag gaaaatatag tttgtgataa agtggttata aggggtactt taagaacctt
     9421 gactcctgaa actagagagt tttcaaaaaa acgaattaga gaaatttgcg attttacttg
     9481 taaaacattt ggaggaagta tttctgttga aattgaggaa ggttatccag ctcttatcaa
     9541 ttcaaaccat ctagtagatt acgtcaaaca aaatgctgta gagttgtttg gagaagaaaa
     9601 tattatcctt aaagactcgc ccactcttgg ggctgaagac ttttcgtatt ttctaagaca
     9661 ctgtgaagga gctttttacc accttggttg tgcaaatcgt gaaaaaaaca ttacttcccc
     9721 tttgcatacc tctacttttg atattgacga agattgcctt attactggtg tcatgctcca
     9781 tgtaaaaaat gtattatcat tctaactatt aatattttta tttattattt tatattgacc
     9841 ctcacatgat gtcatgttat accctaaaat tacaaataat aaattaaata gttggaggta
     9901 atattatgaa agaaatatta ataaatccta taggtaaaat atgtattcaa ggcgaagagg
     9961 tctttattaa attagacaaa aagtatgtac ctgcattaaa tgaactcgac gatttcagtc
    10021 atttaaatgt attttggtgg gctgatgggt ttgataatcc tgaactaaga agtattttag
    10081 aaaccccaaa accttataaa agtggaccag atgttatagg aatatttgca acaaggtcac
    10141 ctataagacc caatccaata gcattgactg ctgtacaaat tatcaatatc gaccatgaaa
    10201 atggtataat aaaaatacca tatattgatg ctaatgataa tagtcctgtt atagacttaa
    10261 agccatatac tccaagcgtt gatagggttg aaaatccgtc cattcctaaa tggtgtagtc
    10321 actggcctaa gagtgtagaa aaatcaggag aatttaattg ggaagatgaa attgaatttt
    10381 aaattacttt tttcttgatt tcattaactt gttttccaca tttattttat ttacacttac
    10441 caagatttta tgtcacatat aca
//
LOCUS       DAEMHW010000134        10318 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEMHW010000134 DAEMHW010000000
VERSION     DAEMHW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.134"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            55..1848
                     /locus_tag="KPK67_002733"
     CDS             55..1848
                     /locus_tag="KPK67_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2459676.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPKGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(1930..3312)
                     /locus_tag="KPK67_002734"
     CDS             complement(1930..3312)
                     /locus_tag="KPK67_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG2459677.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIVGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDSNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            3570..3917
                     /locus_tag="KPK67_002735"
     CDS             3570..3917
                     /locus_tag="KPK67_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG2459678.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            3935..4969
                     /locus_tag="KPK67_002736"
     CDS             3935..4969
                     /locus_tag="KPK67_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459679.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(5018..6067)
                     /locus_tag="KPK67_002737"
     CDS             complement(5018..6067)
                     /locus_tag="KPK67_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2459680.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            6342..7877
                     /locus_tag="KPK67_002738"
     CDS             6342..7877
                     /locus_tag="KPK67_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459681.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(8063..8749)
                     /locus_tag="KPK67_002739"
     CDS             complement(8063..8749)
                     /locus_tag="KPK67_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBG2459682.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            8874..10256
                     /locus_tag="KPK67_002740"
     CDS             8874..10256
                     /locus_tag="KPK67_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2459683.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKEIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
BASE COUNT         3989 a         1290 c         1691 g         3348 t
ORIGIN      
        1 tttacatatt tatatattta gttattcaaa ctattatttc gaggaggtct tgttgtgaat
       61 ataaaagata gattaagtgg attaagaaaa tttatggaag aaaaaaatat agatgcctat
      121 atgattccat cttctgataa tcaccaaagt gaatatgtag gagattactt caaatcaaga
      181 gaatttatat cagggtttaa tggttcagca gggacggtta ttgtgactaa agacgaagct
      241 gggttatgga cagatggaag atattttata caggcagaat ctcaattaga aggaagtaca
      301 ataaaattat tcaaaatggg acaagaaggt tgcccaacta cagatgaata tttatataaa
      361 aatattccta aaggaggaac tttaggattt gatggtagag ttatatctgc tagagaagga
      421 gctacattag ctgaaaaatt atctaaaaaa ggtataaaga tagaatatca gtatgattta
      481 atagatagta tatggccaga tagacctgct ttatcagata gtaaagcatt tttattagat
      541 gttaaatatt gtggagaaag ttttagctca aaattagcta gattaagaga aaaaatgagt
      601 gaaaaaggta cttctactca tgtaataact actcttgatg atatagcatg gttattcaat
      661 ataagaggtg gagatgttaa atataatcca gttgtacttt cttatgcagt aataacttta
      721 aaagaagttt atctatttgt tgatgaaagt aagttaaatg aagaaatact aaatgaacta
      781 gctaaggaaa atgttcaaat aaagccatat aatgatgtgt atgaatttgt aaaaaatata
      841 gacaaaactg aaaaagtttt attagatgga actaagctta gttatacaat ttacaataat
      901 ataccttgtg aagttgagaa agttgatgag ttcaatccag taatgttttt taaagctcaa
      961 aaaaatgagg tagaattaga aaatataaga aattcacatg taaaagatgg tgtagctttc
     1021 actaaattta tgtactggtt aaagaaaaat gtaggtaaga tggaaatcac tgaaataagc
     1081 gctactcaaa aattagagga cttaagaaga gagcaagaag gtttctttga gccaagtttc
     1141 aatacaattg ctgcatataa agagcatgct gcaatgatgc actatagtgc aactccagag
     1201 tcaaattata aattagaggc agaaggatta tttttagtag attcaggtgg acaatattat
     1261 gatggaacta ctgatataac taggactaca gttttaggac ctataagtga tgaattaaaa
     1321 ctacacttta cttctgtagc aagaggaatg ataaatcttt ctaaggctaa gttcttgcat
     1381 ggttgtagag gatataactt agacatacta tctagaagtt gcatgtggaa tatgggaatt
     1441 gattatcaat gtggaactgg acatggtata ggatttgtgc taaatgttca cgaagctcca
     1501 aatggattta gatggagagt agtacctgaa agatttgata gtgcagttct tgaagaagga
     1561 atggtaacta ctaatgaacc aggtatatat atagaaggtt ctcatggtat aagaacagag
     1621 aatgaaattg tagttagaaa agctgaaaag aatttctatg gtcaatttat ggaatttgaa
     1681 gttgttacgt tagctcctat tgacttagat ggtatagtac cagaattaat gaataaggat
     1741 gaaaaagatt atttaaactg gtatcataag ttagtttatg ataaaatatc acctttctta
     1801 actgatgaag aaagagaatg gttaaaagtt tacactagag ctatatagtt tgtactagat
     1861 acaataaaaa atcgaggtat tataatgcct cgatttttta ttgtatattt tattaacttt
     1921 aaaaaatttt taatttgata attctactaa ttttttattc attataagta gtataattcc
     1981 aaatatcaat aaaaatgatg gtatcactat gaagacttgt aacataccaa gttggtctat
     2041 aatagcttgc gtatagccag ataatttatt tgctgcaaat ttgcctaaaa accaaacacc
     2101 cattaggaat gaaatatact tttcaggcga aagtttattt accataccat acccgactgg
     2161 agaaaagcaa atttctccta tactttggaa tgttaaaaat ccaactaccc aaaatacact
     2221 tgctttgtga attgaattag agtctgttcc tgtttgtata actgctacta tcataaacaa
     2281 aaatgctatg gctaatagta taaaacctaa gctcattttt tttggcacac ttaaatcgcc
     2341 ttttttagtt tgagataatt ttacccatac taaagcagag ataggtccaa gaataacaca
     2401 aaggagacca ttatatgaat ctatccatga agtaggaatt tgaaaattgc caacagtaag
     2461 atttatgtgt tcctttgtat agagtgcaat agacatagag gcttggttat atgctatcca
     2521 aaaaaatact gtgaaaaaag ctaacaccaa tattactatt attctttttt tctcaatcaa
     2581 agttaaaggt cttgaagcga gcattttctt atattctagt gattttctta tagactctct
     2641 cccgttaata gggtcattat tcaaaggttt ataaaacttt ctgtttccaa ctataaagaa
     2701 gaataaacct attagaaccg taattgatga aaataaaaat acatatctat atccatacat
     2761 acttatagat aatccgcttt tcatagcaaa ccattcatta gcaactaaac cgcatattaa
     2821 agggccaaat aaggtaccta gattagtagc catatacgta atagagaaag ctccatcttt
     2881 tcttgggtca tcattggtat ataagtttcc aaccatagta tgtaagtttc ctttataaaa
     2941 acctattcct aatccagata aaaatatcat gatgtttata tccatcttac tatttgctat
     3001 gtatcctaga aaataaccaa ttgaaaccat tagcattcca agtataatac agtttttggg
     3061 acctaaaaat ctatcactga cccatccacc aataactgga gagaaataag tccatgctaa
     3121 aaaagatgca agcattgatg cggattctgc tggtgttaac cctaaaccac ctttggagac
     3181 ttgttcagct aaaaacaata ataataccaa tttcatacca tagtatgcaa gtcctacaaa
     3241 ggtaaatatt gaacagcaga aatataaatc tctaggttgt ttaagttttt cttgcgtttt
     3301 taaaactgac atatgtcgag aacctccaga taaatagttt aacaaaattt taccatacta
     3361 tatgagtata tgtttttttc atatagacgt caacttgtaa gataatttaa ttaagtcgaa
     3421 atatatgtaa aaatagtatc atttttgtgt attcagtaat atttttttat gataatatat
     3481 gttattatta tattaaaata caaacatatt tatggaaagg atatgccaat ttagtagtta
     3541 gattgattgt ttggcaggtg ggatgagcta tgataagaaa gaaatctgaa ttacaagtag
     3601 aagtaaaaga aaatttaaat ggtggtatag gaaaggttaa gcttgaaaac ataatacaaa
     3661 atgaagagct taaaggaaaa ggaagactat ttaaaagagt agctttacca gtaggaagtt
     3721 caattggtgt gcatgaccat acaacagatt ttgaagtata ttatatacta aagggaaaag
     3781 gtaaagtatt tgataatggg gaatttgtag aagtaaatga gggtgatgta gtgtatacag
     3841 ctgatggcga aaaacattct atagaaaata taggagaaga agatcttgag tttgtagcac
     3901 tagttttata tatgtaaaat atggaggagg agtcatgaca ggcaagatag aagaaattaa
     3961 ggacaagata aatagatttt gcaactccaa tttaaatcaa gaatacaaag atataagctt
     4021 taaaattttg caaaaccttt tagataataa taaagagata attcattctt ctagagctga
     4081 tatatggtct tcagctatac taaatatagt tttagaacaa aatcttttat acaataagaa
     4141 gcatccacta catataacaa aaaaagagtt ttcaaaacaa ataggtgttt ctttaaatac
     4201 tataaataat aaatcatctt taataaaaga tgtttgtagt attgactttt taaatcaaga
     4261 tactatagct atttctaatg tagaattttg ggtaaaagag accaattcca aatcaaagtc
     4321 tgtagattta gatttagaaa agaaaaaaat actatataaa agatatatta agctatcaca
     4381 agatgcgaat agtgatattg aatcaattag atatatggag gaagctgtaa atataggtaa
     4441 aagtatgatt actaaagaag ataaagaact tggattttgg aaaggtttat ctaccagagc
     4501 ctatatgata gcacttgaat cccttgctag aaagttagaa agtattaata atctaaagga
     4561 gtccaaaaaa gtattagaat atttaataga aattaatcct agtgatgagc agggaataag
     4621 atacaaatta ttaaatgtat taattagatt aaatgataga acttcaataa ataaattatt
     4681 tgaactttat aaagaagaaa aatctgctac atggatgtac agtaaagctc tttattactt
     4741 taaaaataaa agtatgtttt tggcaaatga ttctattaag gttgctagaa gtaaaaataa
     4801 gtacgttgga ttatatttgt tagattggcg aaatgctttt ggtagagaat ttgttacaga
     4861 ggaagaaaag gcagaagctg tctattacta tgatgaaaac atagtgattt ggaatgaagt
     4921 taaaggttct atggattggc tattaaaaaa aatgttggaa ttttcataaa aaaaactact
     4981 ccttactttt atataagtaa aaggagtagt ttttaattta tacatctagt tttaaatctt
     5041 caggttttat tagacctttt tttctcataa attctgaaat caacaaagtt acaatagcag
     5101 gaagtataaa attaagtaaa agtataccaa cccacataga tatacctcct tttccagcac
     5161 tttgcatagc agttactgtt ccaaattgtc caaccaatcc acaagtgccc ataccagaac
     5221 caataggaat attctccatt ttgaatattg ttgtagataa tggtccaagt aatattgaag
     5281 ctacagtagg tggtatccaa attttccaat tttttattat attaggtact tgcaacatag
     5341 atgtcccaag accttgtgcc acaaggccac caactccatt ttccctaaag ctcataacag
     5401 caaagcctac catttggcaa caacaaccta cagttgctgc cccaccggca agaccaccta
     5461 aacttaacat catacaaata gctgcactac ttataggtaa tgtaagagct attccaacaa
     5521 gtgcagagac taaagctccc attattatag gttgcatagt tgtagcttgc attattatat
     5581 ttccaaatga agtcataaaa gcagaaacag ctggaccaat aagttcagct acaagtactc
     5641 caattagtat tgtcacagca ggtgtaacca atatatctat ttttgtttct tttgaaacta
     5701 attttccaac ctctacagca aaaagtgtag cgacaaatgc tccaacaggt cctcctaatt
     5761 cattaccaca agctccaaca agtgcacaag aaaatagtac aagtggaggt gcttgaagtc
     5821 catacgcaat agccactgct atagctggac cagtaacctg tttagcaaga gggcttataa
     5881 cttcagtaaa aagagaaata ttgaaattat ctcctaatgt attgaaaatt gttccaatta
     5941 aaagggacga aaaaagaccg agtgccatat aacttagtgc gtcaataaga tacctttgaa
     6001 cagaaatttc aatatttttt cttttagcaa atgatttgaa atttcttttt ttataattga
     6061 tttccaattt aaaatcctcc tcatataata taagtataat ttctttttaa ttaattatat
     6121 aacttttcag ctgacaagac aaatgttatt tcactaaaat tagactattg taatagtaag
     6181 aaattaacat taattttagt tttaaatttt aaatttatgg tttataatta gtatagggat
     6241 attctgaaat ataataattt tccagtttat cccaatattt aacttaggta gattgcttac
     6301 aaaacaaatg attatatatt aggctaaaag gaagtggata gatgatattt gtaaaaaata
     6361 agaaatacag aaaaattgcc ataattatat ctattcctat attgcttgtt gcgcagtact
     6421 acctttttaa atttggaata cttagaccaa tggtaaaggg agtagaaatt gaaattgtag
     6481 atggggaata tgttaaagat atagataaat atgtagtaaa attaaatgaa acagttactt
     6541 tgtctacagg tgaatatatt aaggttcctt cgtatgctaa aaatccaaat atttggttta
     6601 atgtattgga taactcaggg actttaaaaa tagatggaaa taaaatgaca gcattaaagg
     6661 ttgggtattc ttctgtgggt atcatgaaaa attcaagagt attaaaaaag gctactataa
     6721 aagtagtaga cccagaggta gaatcactag atatggagat taatggggat ttaaaacatg
     6781 ttggtgatag tgctactata gaaagtacaa tagaagttga ttatgataag tttaaaaaat
     6841 catataagcc agtttataag agtagtaatg aaaatatttt aaaaatcaaa ggaaataaag
     6901 taagtgcagt tggagttggt aaagctacaa tatatgctaa gtgtggaaat aaagaaattg
     6961 agagaacatt taggattcaa gctagagttg ctgaaataaa tatggatgat ataaaaatag
     7021 aagttgacca aaatgaaaaa ttgaaaccag atataataac ttctccacct ggtttagaac
     7081 caccaaaaat atattatgag ttagtagatt caaaattacc tgtagaaaga gcaataagtt
     7141 taagtagtaa tggaaatgtt gtaggtctta gagaaggtaa agatagggtg agaataactt
     7201 gtggagataa atctaaagta ataacagtaa cagtagtcaa agaaactata acaaatgact
     7261 atatacaaaa cttggttggt tcacataaaa tagtaaacaa taaagttatt attaaacttg
     7321 aatgggatta tatgaaagat gtctttgatt atgatatata tcttaaagat aagttgtcag
     7381 gagaatcatt taagaaaatt aaatctataa ctataaatga aagtgattta ggtaagtcta
     7441 acaaggtata tgcaactata gaagtagata tgaagggtaa tcaaaatata gattttgata
     7501 tttatgtcat agggaaaaag gattcagata caactaagcc aagtaatgta ataaatatta
     7561 ccaatggaaa tggaaattca aataatgata ataatattaa agatatgagg gtagaaaata
     7621 tatcagctaa tgttgataga gataataatg tcattagagt taactggagt agtatatctc
     7681 ataaggactg tacttacagt atatatgtaa aaaataatac taatggagat ggagaattta
     7741 ctctatatca aagtgatata cagggaaatg agcatactat aagtattcct gaaggagatt
     7801 taaatctaga tatttatgtt gttgcaaatt ataatggaaa tagctctcaa aatagtgatg
     7861 ttataaatgt aagatagatt tgtatacaaa ttgaaagtgt tgagtttatt taaatattaa
     7921 attaagaaaa aggatagtat agggtaatta tactatcctt tttttgttac aatatatagg
     7981 tttaaaatct ctagtaatat aaattataaa aagtcttatt tacacaaatc tatagatatt
     8041 ctttttatta atcaatattg tcttaaattg aacttagtgc ggcttctatt gcatgtatta
     8101 ctgtcgtatc aaatagtgga atagaagtgt cattttgttt aattaaaaga cctatttctg
     8161 tacatcctaa tataacagcc tctgcacctt gtttgccaag cttatctata atagataaga
     8221 aagctttttt tgaagattca gatataattc ctaagcaaag ttcattaaat attgtgtcat
     8281 ttactattat acggtcttct tcatttggaa ttaagacttc aattccatta ttaataatta
     8341 catttttata aaaatcttgt tccattgtat atttagtacc taataaggcc acttttttta
     8401 tttctttttc ccttaatacc gtagcagtta catctgctat gtgaagtaat ggaatatgta
     8461 tactttcttg tattttatct gataccttat gcatagtatt agtacagata acaataaaat
     8521 ctgcccctgc ctcttgtaat ttaattgcag catcagctaa aattttggcg cttttttccc
     8581 aatttccact agattggcat tcttcaattt cttgaaaatc tacactgtaa agtatacatt
     8641 tagaagagtg tagtccacct aatctttctt ttattactgt attaataact tgataatatg
     8701 taatggtact ttcccaactc ataccaccaa ttaagcctat agtcttcata ttatacctct
     8761 cttatttatt tattttgaaa tagctattta caaatacaag tatagcatct aaaatagaag
     8821 catacaataa taaaattgta tggagtacaa ttataattaa gaggagtaat tatatgccaa
     8881 taaattcttt tgaaaactat cctatgacat ggaaaccaaa gttagataac cgaaaaccac
     8941 caatatataa aactttagca atgttattag aagaagatat taaaagagga aatttaaatc
     9001 ctggagataa actgcctcca caacgggaat tagcagattt tctagattta aacttaagta
     9061 caataacacg agcatttaaa ctttgcgaag aaaagggact aatatgtgca aaagtgggaa
     9121 agggaacatt catttcttcg gatgtaaatg taagtaatac attattatat caaacggaat
     9181 caaaagaaat aattgagtta gggacagttc atcctccata tgagcaaaat acatatatta
     9241 ttgactttat taaaaatgta ctaaaacaac cagaaatgga taggttttta caatacatgt
     9301 ctccatctgg cacatatatg caaaaaaagt caattgcaaa atggatagaa agaaataatg
     9361 tatacaccag tgaagaaaat atattattat ctacaggagg gcaaaatgct atatgtgcaa
     9421 ctttactagg attatttaaa gctggtgata gaattgctac agattcttta agtttttctg
     9481 gaattaagtc aatagctaaa atgataggta ttcaattagt cccaattcct caagaaaata
     9541 atgaaatatc gattgaatat cttgaaagtt attgtaaaaa tgaaaatata aaaggcatat
     9601 atcttatacc agattatcat aatccaacga cacatactat gtctgattta tctcgtaaaa
     9661 agatagccaa gatagcaaag caatataata taataattat tgaagatgct ataaatagca
     9721 tttttagaga cggtatacaa actcccatat tttctttagc tagtgataat actatatata
     9781 tttttagtac atctaaattt ttatgtgctg gtcttagagt agcatttgta gttgctccaa
     9841 aacgatatat agaaaattta gaaaatgcac tatataatat gaatctaatg gtttctccat
     9901 ttacagcaga aatagtacac cgtttattat attctcctat tatagataaa attatagaag
     9961 agaaaagaga tgcaattata gaaagaaatg aagttgctga taaagttttg tcagattata
    10021 atttgattgg tgataagaat tgcagttttc gttggttact tcttcctgat gaattggatg
    10081 gaaaatcatt tgaaatttct gcaaaaaatc taggagttca agtatattgt gctgaacgat
    10141 tttcagtagg aaactctaca gttccaaaag cagttcgtat ttgtgtaaca gctccaaaag
    10201 atgtagaaga attagaaaaa gggcttaata taataaagtc acttcttaag agttaaattt
    10261 attttactat tatttaatta aataaataaa aaggcggaga aatttattat aataaacc
//
LOCUS       DAEMHW010000135        10261 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEMHW010000135 DAEMHW010000000
VERSION     DAEMHW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.135"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            70..987
                     /locus_tag="KPK67_002741"
     CDS             70..987
                     /locus_tag="KPK67_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG2459684.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     ICSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAISGEECIKAQRLLEKIINSVK"
     gene            complement(1110..2039)
                     /gene="pfkB"
                     /locus_tag="KPK67_002742"
     CDS             complement(1110..2039)
                     /gene="pfkB"
                     /locus_tag="KPK67_002742"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG2459685.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEIKKLS"
     gene            2308..3033
                     /locus_tag="KPK67_002743"
     CDS             2308..3033
                     /locus_tag="KPK67_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2459686.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            3116..4270
                     /gene="nagA"
                     /locus_tag="KPK67_002744"
     CDS             3116..4270
                     /gene="nagA"
                     /locus_tag="KPK67_002744"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG2459687.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNITDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNKVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICMYKVNK"
     gene            complement(4453..5610)
                     /locus_tag="KPK67_002745"
     CDS             complement(4453..5610)
                     /locus_tag="KPK67_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBG2459688.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGASKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKIVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYVEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            5764..6333
                     /locus_tag="KPK67_002746"
     CDS             5764..6333
                     /locus_tag="KPK67_002746"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBG2459689.1"
                     /translation="MKKEFRKKVIESRKNKSANFVISNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGEYGIR
                     EPNEKSPTVDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            complement(6515..6931)
                     /locus_tag="KPK67_002747"
     CDS             complement(6515..6931)
                     /locus_tag="KPK67_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459690.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(7156..8250)
                     /locus_tag="KPK67_002748"
     CDS             complement(7156..8250)
                     /locus_tag="KPK67_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459691.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDRLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPIIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDNHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            complement(8673..9614)
                     /locus_tag="KPK67_002749"
     CDS             complement(8673..9614)
                     /locus_tag="KPK67_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG2459692.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIVKTVKEAIIAKRM"
     gene            complement(9607..10179)
                     /locus_tag="KPK67_002750"
                     /pseudo
     CDS             complement(9607..10179)
                     /locus_tag="KPK67_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
BASE COUNT         3596 a         1703 c         1265 g         3697 t
ORIGIN      
        1 aagtttacaa atttatgtgt ctgaattata atcaattctg taaatataaa aaccttagga
       61 gaaagtatta tgaaaaatat aaaaatggga atgataggtc ttggctccat agctcaaaag
      121 gcgtatttac ctatattaac taagtctgag agatttgagt ttgtaggagc ttttactcca
      181 aataaagtta aaagagaaaa aatctgtagt gattatagaa tcatgccatt cgattctatt
      241 gaatccctag ctaaaaaatg tgattgtatt tttctacata gctctacaga aacacactac
      301 gaaattataa aaatattatt aaatttaggt gttcatgtat atgtagacaa acctcttgct
      361 tcaacagtaa gtcaaggaga agaacttatc gagttaagta cgaaaaaaaa cttaaattta
      421 atggttggat tcaaccgtag attctgtcct atgtacaaag aaattaaaaa taatgctaca
      481 gaaattgtat ctatcaatat atgtaaacat ggattaaatt ccttaagaaa tgttagattc
      541 gacagtactc ttatagatga ctatatacat gttattgaca ctgctttatg gttagcaaat
      601 gaagatgttg aaattagtgg agaggattta tttttaacag acaataagaa tcttatattt
      661 gtaagtcata aacttaaagg taaaaatttc tctataaaca cctctatgca tagagattct
      721 ggaacaaagt tagaacaagt tgaaattctt tcaaagggta agattcaaag agttaagaat
      781 cttaatgtac tggaaataga agaaggtgga aacctaactt taaagcaaag tggtgcatgg
      841 gtaaatatat taaaacaaaa gggttttgag gatatatcaa atcattttat tgattgtata
      901 gaaaataata taaaaccagc tattagtgga gaagagtgta tcaaggctca aagattacta
      961 gagaaaatta taaattcagt aaaatagata tttattataa aaataggtta aaaatgaatt
     1021 tttctaacct atttttatat aagactatat cttttatatt aaaatctatt acaacaacat
     1081 taaatttatt atattcccat taaatgtatt taacttaact tttttatttc aactaaatct
     1141 tgatatttat caatttctcc caaatctaca aatccagttt ccatatttaa agcattactt
     1201 ataccacaag catttgcaaa tttaagtaaa ttttctattt tataacccct taaaatacct
     1261 acactaaatc cagccactgt gctatctcca ctacctacag tatttataca ctttacaact
     1321 ggtactttta catcatatac attatcttcg tttaaataca ccataccatc ttttcctaaa
     1381 gatatacata cattctctgc acccatttca ataagttttt ttcctgataa gataatttcc
     1441 tctcttgatt ctatatttat tcctaataaa tgtcttattt catctatatt tggctttatt
     1501 aaatatggtt ttgattttaa agctattttt aatgcttctc ctgatgtatc taatataaat
     1561 tttatattat ttgctctaca tatatttcct atcttttcat aatagtctat cggcatattc
     1621 tcacaataac taccagaagc taccagtatt ttagtatttt ttagtacttc ctctatatca
     1681 ttttcaaatc tctctaaatc gcttttttct actattggtc ctttttctaa aaattctgta
     1741 ctaactttat tgtcctcaat tatgtttaag caatttctag tttcctgctc tatttttgta
     1801 aatttatttt gtatatctag ttttctaagt tcatttttta taaattctcc attaaatcca
     1861 cctagaaatc ccatagctaa aggattttct tttaaaattt tacatacctt agctacgttt
     1921 aatcctttac ctccagcagt tgcattctta cttactaccc tttgcacctc acctatgttt
     1981 atattattca ctctatacat tctatctata gatgggttga atgttattac tgtaatcata
     2041 ctagattctc ctcattatta atttttactt ttctaaacaa taaattaatc tgtaaaataa
     2101 aaaatacctc atctataata tacccaaagt ggttattacc aatttgaata taaattattt
     2161 tacttgtttt gtcaatgtac cataatttta taataaatta atagccttac ttattgaatt
     2221 ttgattaaac tttattcatt gacataaata attctatatt ctataatcaa attgtggtaa
     2281 taaccactta gaaaggacta tatttttatg ataaaaagaa atgataaaag accaatatat
     2341 gaccagcttg ttgagatatt aagaactaag attgaaaatg agatggaacc aaacgataga
     2401 atgctttccg aaagaaaaat ttgtgatgaa tatggagtaa gtagaaccac agttagacta
     2461 gctatggctg agttggaaca tatggggtat atatacaaaa gacatggaaa aggaactttt
     2521 gttgctgcac taagtcaaaa ttctcaaaac ttaatggaaa gttatagctt tacagattat
     2581 atgagaacac aaggaaaaaa gccaggtaca aaagtattat catttgaggt agtagaaagt
     2641 acaaagtatt tttctgaaaa attaggtata actcctggtg aaaaaatgat aaaaataatg
     2701 cgtttaagac ttgctgatga ccttcctatg atgcttgaaa gaacatatct tcctatgaaa
     2761 gaatttatag gattgacaaa agaaaaaatc gaacaaaaac ctttatatga aatatttaga
     2821 gaagattatg gcgagattat taaagttgct gatgaagaat tttcagctgg aataatctct
     2881 gataaggaag ctaagttatt ggaagtacca ggagattcac cttgtctgaa attacttcgt
     2941 accacatata atgatgacaa tatagttatt gaatttactt tgagtgttgc tagaagcgat
     3001 aaatttgtat atagagtaag acatgtaaga tagtaagtta aatagctttc aagaaaatag
     3061 aatgatatta taaacgtcaa tgccacaatg atagtataat tataaggagg ttattttgaa
     3121 aagttttatt tatgcagata aattttttat gaaacatgga atcaaagaaa aaggttacct
     3181 tagtataatt aatggcaaat tcggtgaatt tcaacaagaa agacctaata ttactgataa
     3241 tgttatagat ttttcaggta aatatattgc tcctggctta gttgatactc acattcacgg
     3301 tcttttaggt gcagatgtca tggacaatac atttgaagca ataaacacaa tttcaaaagg
     3361 cttattaaaa tatggagtaa catctttcct acctacaact ttaactgatt ctgtagatac
     3421 actaagtgat tctgtagaaa atataagcaa tagcttcaat aaagttgagg gtgcaaaaat
     3481 acaagggatt tatttagaag gtccattttt tacagaaaaa tataagggcg ctcaaaatga
     3541 aaaatatttt aaagaccctg acatcaatat attaaaatta tggcaaaaaa aatcgaaaaa
     3601 tttaataagg aaaatagcaa ttgctccaga acgtaaaggt tcaatagaat taacaagata
     3661 tgcaactaaa aataatatat cagtttctct tggtcatagt tcagctacat ttgagcaagt
     3721 taaacaagtc gttaattcag gtgctaaagt gtttgtacat acatataatg ggatgagtcc
     3781 tttaaatcat agagaacctg gaatggttgg ggctgctatg gtactaaaag atacttatgc
     3841 tgaattaatt tgtgatggtc atcatgtaag cccaatagct gttaaaataa tgatggatgc
     3901 taagtctagt aataacatag ctttaattac agattgtatg agagctggtg ctatggagga
     3961 tggaaaatat actcttggta agtttaatgt aaatgtaaaa aatagtgttg caagattaaa
     4021 tagtgggagt ttagctggaa gtgtattaac tatggacaag gctgtgagaa atatagttga
     4081 ttggaacata tcaactttag aagatgcaat aaaaatggct acatatgtcc ctgctttaag
     4141 ttgtaatatt gataatgtct gtggctctat atatcaaggt atggatgcag actttatagt
     4201 aacagataat gactttaata tacacgaaac ttatataaat ggtatctgta tgtacaaagt
     4261 taataaatag attcaatata aagttataga ggcttcctaa aaatagagat aagcctcttt
     4321 ttcattgtta aaatgatgaa aatacatttg atttcataca aaaagagtgc ctcatgaact
     4381 aaaaagttca ttttgagaca tctctcttat aatgttcttt attttatagt ataagtttat
     4441 aaaaattaaa tcctatttat caatctgttc tctaagccac ttagttgcta catctaactg
     4501 tttattttca tctttttctt caatagtagg ctttatacct ttttcattga tataatctcc
     4561 attaggtgta aaatattgag cagtagttaa cttgtatcct gttccatcct tcaatcttac
     4621 aactgattgt accaaccctt taccaaaagt tgtagtccct acagaaatac cttctttatt
     4681 atcaacgatt gcagctgtta atatttcagc agcagaagca ctttctccat tagttaacac
     4741 tactattgga agtcccaatt tttctttatc agatttcaag tactcagtat ttcccttatt
     4801 atcttttgta tatactatag ttccttcacc aattaactca tcagcaactt ctttacatat
     4861 atctagcatt ccaccaggat tatttctaag gtctattact aatcccttga tattttttgc
     4921 ttttaattct tttaaagctt tatcaaagtc agtatatgta cttgatataa attgggtaat
     4981 ttctacatat cctaaatcat tatcaattac tttactttct atactcttct caattatctg
     5041 gtctgttttt actttaaagt tcaattgttt atcttctctt aatatagtca gttcaactat
     5101 tttccctttt ttacccttca tcatacgaac agcttcatct gaatttttat atgagacaga
     5161 ttttccattg acttttacta atttatcccc tgattttaca cctgctttct ctgctggaga
     5221 atctttcatg ggaactacta ctaagccacc atcttttgat gcccctatat acatacctac
     5281 accaacataa gaaccttcac tatcattaat tagattctcc atttcatctt tagtataata
     5341 accagaatac ttatcatttg ttcctagaaa tagacctttt gatgctccat ttaccaaatc
     5401 agtatctgat acttttttat aaaaatcttg ttttatgata gattctaatc ctaacaactt
     5461 atcatatttt ttatagtctt catagagttc tttcgatatt acaactttat tccccaatgt
     5521 caactgaaat gctgatgtaa ccatagctgt tataattact aaaatcaccc ctaaaaaaat
     5581 agctttcttc tttgaaatca caagatacat ctccttttac ataaactatt attattatat
     5641 gactagctgt tatttttatt acatttaccc aaatatattg ctttattaca ataaattata
     5701 ctaaaattta tattaaatac aattgctgta cacattataa catattaagg gaggaattat
     5761 tttatgaaaa aagaatttag aaaaaaagtt attgaatcaa gaaaaaataa gagtgcaaac
     5821 ttcgtaatta gtaattcaaa tttaataact gaaaaattac ttcaaatgga agatataaga
     5881 aatgcaacta atataatgct ttatttaaat tttaacaatg aagttcaaac agataattta
     5941 atcaaaaaac ttttgtctct aaagaaaata gtatcaagtc ccatcacaat aaaagaaaat
     6001 catacattaa tccctactca aataacagat ttaaaagagg gattgagaat tggagaatat
     6061 ggaataagag aacctaacga aaaatcacct acagttgata ttaaggcctt agatgttgta
     6121 attgtaccag ctgtagcata tgatatacat tgctatagat taggatatgg tggaggattt
     6181 tatgatagat ttttagaaaa cttaagaaaa gatgctatta ctatcggcat agcatttgac
     6241 ttccagatat tcgattctat tccaaaagaa actcatgatg cacaattaga ctacatagtt
     6301 actgaaacta gaattatcac tccaaatagt tagaatatta agtatttcca gaccccattt
     6361 aatgtagtaa taaatactac tgttaatatc ttactttata agcattttaa gtcatggctt
     6421 ttacaacaat aagaaccata tacaatttac ttacattaat aaaaaccatg actttattgt
     6481 acaatatgca gttatttaat tataaaatac cttcttattt cgttgcattt aattctattc
     6541 tatctggaag ttcactcata tttgtttcaa caactatgta tggaaaagaa tcttccttat
     6601 ctttatactt tatcttgacc ctcaaaacat cctttccttc ctcaaccaca ctttctattt
     6661 tgtccatttc tataccatat ttattatttc ctctagtcac tataatgtat attttgttat
     6721 cgattttagc cgctaatgct ctttcttcat ttgtatactt atccatcaca tctagtatct
     6781 tttctggtat tgaatttctt tgtaatactg tataatctat gggtttagat ttctctacaa
     6841 acatacttgg tatgaatata aatcccccta taacgaatac aagagctata actccataaa
     6901 ttatcccttt aatattaaac tttacattca atcttatcac tcctcccata tattctaaat
     6961 atatgattta taaacttatc ttagactcta aattattgat tagtatatat ttatacataa
     7021 ttataaatat atgtaatgta actaaaaaac atgttttttc tatttcataa taaacaagct
     7081 aaaaataaaa agagctatat aaaaacattt tcattgtttt catatagctc ttgagtttac
     7141 aatattaaaa aatatttatt tgtcactgat tccttcttga tatccagatg tttttgtaat
     7201 ctgtctataa agaacacaaa ccagtattat gacgcccata tatccagtat atggatataa
     7261 aataccaacc agcttatcaa atggtaggaa tccacctata agacctaata tagctgctat
     7321 taaagttatt aatttaaact taggatgatt atcatctaca aatctattag ttactgacca
     7381 taataaaggt acagctgttg tatatatacc taatataaga attattgaga ataaaactcc
     7441 aattattggt gaaatcttat ctgctaagaa taatgatggt attgcttttg aatataagtt
     7501 tcctatatca gaaagaatag ctaagtgcat cattatagct gcagccataa gtgctactcc
     7561 acctaatata ccaccacaaa tagcttcttt tttgttagca gcacttgctc ctaatccagt
     7621 taagaatgcc ataacaacaa ttgtgttata agtagtatat attacaccag atagataggc
     7681 atttggaact ggtttagcta tctttacagt gctaagaact tgtgcagctt ctcctatatt
     7741 acctatattt ttagctaaac ttataccacc aactaaaagt gtaaatatta ttattatagg
     7801 ccctattcca cctagtatct ttgataatct atcaagacct aatgaaacag ttaataatgc
     7861 tactactgcc atacctactc tacctacaaa tggatttaat ccatagtatt cagttaaagt
     7921 tgctcctgct cctgaaatca ttactacgaa aacaccaaat aagaataaag gtccaaacca
     7981 ttcaaagaat attcctaaat acttaccaca ataaacacga taaattttga taggttcttt
     8041 aagttgaagc tctttacctt ttgacataac tgaagcaccc atccatgaga ataataccat
     8101 tgaaattatc ccaccaataa tacctgcaaa accataaaaa gagaagaatt gcattatttc
     8161 ctgtcctgtt gcaaatccag aaccgattac tgttgctaca tacgcacctg cgaaactgat
     8221 gactgtttta acatttactt tttctgacat atctcagtta cctccccatg tgaaattttt
     8281 tcttctgctt aaacaattcg ataaactacc tacaatttcc tttaacataa aatacatgat
     8341 aactgtttta tagcttttta gtataataac ttaaaactta taatctaatt ttatacccta
     8401 gtcctccttt cataattttt ttagattatt ttatctaagt agtcattata tataaaatta
     8461 atttctctat aaattatata ttagtcatat ttttctgaat agtcaatata tatttgaata
     8521 ttttctctat ttttgtaaaa atttgaatat tttattatat ttttgatata ttgatatcat
     8581 ataaaaaaaa gagagatata aattaatata acactttata cctctctttt aaatatatta
     8641 tattattaac aaacatgaat aatttatatc tcttacattc tttttgcaat aatagcttct
     8701 ttaacagtct ttactatgtc atcacaagtt aattcatatt tttttaacaa ttcatttggg
     8761 gtaccagatt ctccaaatgt atctttaatt ccaactttct ttacaatggt tggacaagac
     8821 tctcccacta cctcacttac agcagaacct aatccaccaa ttatgctatg ttcttctaca
     8881 gttacaatag cattcgtttc ttttgctgat tctaatatta aatctttgtc tataggtttt
     8941 atcgaggaca tgtttataac tctagcactt ataccttctt cttgcaactt ttcttgtgct
     9001 aatagagctt catttaccat aattccacaa gctataattg atacatcctt tccttctctt
     9061 aagactgtac cttttcctat ttcaaattta taatcttcat caaataaaac tggtactgaa
     9121 cttctcccta atctaacata aacaggtcca ttatacttag ctatttcaaa aataattttt
     9181 tctgtttcaa ctccatcagc tggaacaaga actgtcatat ttggtatagc tctcattata
     9241 gcaatatctt ccacactttc atgagatgct ccatcctctc caacagtaag acctgcatga
     9301 gtagcacata tttttacatt tagctttgga taacaaactg aatttcttat aatctcaaaa
     9361 gcccttcccg tagcaaacat agcaaaagta cttgcaaaag gtattttacc agcagttgat
     9421 aatccacaag cagcgcctat taaattttgt tcagctatac ccatattaaa aaatctatct
     9481 ggaaatgact tgtaaaaatc atgtgttttt gtagactttg ataaatctgc atctaatact
     9541 acaacattgt catttatttg acctaatttt actaatgctt taccatatgc ttctctcgtc
     9601 gccattttac tcattgtcag cacctcctaa ttcaagcaag gctttttcta gctcctcatc
     9661 acttggagct gtcccatgcc atcctgcttg attttccata aaagatatcc ctttgccttt
     9721 tatagttttt gcaactatca ttgtaggttt tttaactgtt gatttagcta tatcaagagc
     9781 cgcaaatatt tggtcaaaat catgtccatc tatctcaatt acattccaac caaaagcttt
     9841 aaatttatca actattgaac ctaaactcat tacgctttct atatctccat ctatttgaag
     9901 tccattatta tctaaaaaag caattaaatt atcaagttta taatgtgcag cactcatagc
     9961 agcttcccaa acaattcctt cttgaacctc accatcacca agcaaagtat acacattcca
    10021 cggagcatta tccaactttg atgccatagc cataccacat gcaactgaaa aaccttgacc
    10081 taaagaacca gtagatattt ccacacctgg cgtgcctttc atgtctggat gaccttgtaa
    10141 catccttcct atcttcctta atccattcag ttcttccttc tgaacctccc atgactaaag
    10201 tcacagggtt cctgcttcat agaattttgc atactaaaat atgtcttact aattctccac
    10261 a
//
LOCUS       DAEMHW010000136        10238 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEMHW010000136 DAEMHW010000000
VERSION     DAEMHW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.136"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            244..1674
                     /locus_tag="KPK67_002751"
     CDS             244..1674
                     /locus_tag="KPK67_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2459693.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1777..2880
                     /locus_tag="KPK67_002752"
     CDS             1777..2880
                     /locus_tag="KPK67_002752"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG2459694.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKAENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYIRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            2895..3131
                     /locus_tag="KPK67_002753"
     CDS             2895..3131
                     /locus_tag="KPK67_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2459695.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3148..4197
                     /gene="vorB"
                     /locus_tag="KPK67_002754"
     CDS             3148..4197
                     /gene="vorB"
                     /locus_tag="KPK67_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG2459696.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEAYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4199..4939
                     /locus_tag="KPK67_002755"
     CDS             4199..4939
                     /locus_tag="KPK67_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG2459697.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            4942..5481
                     /locus_tag="KPK67_002756"
     CDS             4942..5481
                     /locus_tag="KPK67_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG2459698.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5605..7704
                     /locus_tag="KPK67_002757"
     CDS             5605..7704
                     /locus_tag="KPK67_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBG2459699.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(7971..8486)
                     /locus_tag="KPK67_002758"
     CDS             complement(7971..8486)
                     /locus_tag="KPK67_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBG2459700.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8578..9789)
                     /locus_tag="KPK67_002759"
     CDS             complement(8578..9789)
                     /locus_tag="KPK67_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG2459701.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKFLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         3870 a         1248 c         1871 g         3249 t
ORIGIN      
        1 acaagaaaat aataaaaaac aaacctaaat taagtctaaa tagcttttta gttaatttta
       61 gtagtaaaaa ataaaactta aaaagtgttt gacaattatg aattttgaga atataattaa
      121 attaaaatta tacacatata aaaatatact cctgaatcaa gaatgagata tttaagattc
      181 aaaatatata ttattatacg tgaataataa aaagtaagaa aattttataa gggaggggta
      241 gatgtgagta gtcaagaaag caaaccaaaa ggaaatctta tatcaaattt tagagatgat
      301 atgagagacc ctaagaaaag agcaatatac acatattcaa gttctataaa cttagcgtac
      361 aattttatag gctactttgt atcaactgtt acaaatattt ttttaactga tgtggcaaaa
      421 cttggtgtaa ttatggcagg tggtatgcaa attatacaat ctttcataaa aattgctgta
      481 gctcctataa caggtacagt atttgacaaa caaccattca aaaaaggaaa gtattatcca
      541 tggcttagat atgcaccagc agcgttagca ttaacatatg gaatattttt catagttcca
      601 ttaatgaaaa taagtccagc attactttta cctttgatga cagctttatt attaatagca
      661 tcttgtgctc agcaagtagt tttcactata atttatgcta tttaccctgt agttgctaag
      721 acacctagag accgagttat aggctcaact actacgaata tatttaaaga agttggtaaa
      781 ttcttagtag gtttttcata tccgttatta ttagttttat ttgcagatgt attaggtgga
      841 gaaggaatgg gttatttagg tacctactta ttctttgcag caggatgcct tttagtattt
      901 tggttttctt caacagagat aataaaatct ggagcagaag aagaagttct tcaaaaagaa
      961 gagaagccaa agattaaagc aagtgaaatg gcaaaagctt tattcactaa tgtaccactt
     1021 atgatggcat tttgtttaga atttcttata tgtataagat ctataggatt aggacctttg
     1081 gctccatatt attttaaata tgtaatagaa gatgaacgtg gattagcagt atttttatca
     1141 atcatgcctt tggtaagtgt agcattcatg tttttcgcac cagtgtttat aaaaatctgt
     1201 agagagcaaa aactagcttc aataatttct ttttctatat gcgcattatg ccacttattg
     1261 gttgcatgga ctccttgggg gaaaactact ttaggagtaa ctatgttgct ggcaattggt
     1321 ggaggttttt caaatgtagt atcaataata aatcttaatt tctttgccgg ttcttgtgat
     1381 tatgggcatt ggaaatcagg aaaagactta cctggcttaa gtatgtcact atatccagtt
     1441 gcaatacaag taggagtatt acttgcaact actataagaa cagttttaat gaactcaatg
     1501 ggatatcaag ctgatatggt agtcacagaa gcagttaaat caggttttat aaatatgatt
     1561 tcttattcta tggcaatacc tttaataata gctgttgtta tagcaatact atatccagta
     1621 agtgacaaga aattaaacca aataagagaa gagttaaatc aaagaaatgc ttagttaaaa
     1681 gaaagttaag tagatggtta atttaagcta ttaaagtaag tagacgcaat agcgtttata
     1741 taaatgaaaa tacatataca gagggggctt ttaaaaatga tagaaaaatt attgagaaat
     1801 accgtaaaag aattacatca atatgttcca ggtgaaccaa tagaaaaagt taaggaaaaa
     1861 tatggtgtaa aagaaataat aaaattagct tcaaatgaaa atcctctagg accatcacca
     1921 aaagcaatag aagctatgat tgaaatgtta aaacaaggtc aactttatcc agagccagaa
     1981 gctaacgaac ttagaagaaa attagctgaa aagctagatt taaaagcaga aaattttatt
     2041 gttgctaatg gtgcagataa cgttatcact ctaataggtg aggcttttat aaatagagga
     2101 gatgaagtta tatattgtaa cccaacattt ccatcatata gaactgcaac tataaaaaat
     2161 gaagggatac cagtagaagt acctttaaca gaagattata agtatgattt acaaggtata
     2221 ttagataaga ttacagataa aacaaaatta atatgtgtat gtaaccctaa taatccaaca
     2281 ggaacaattg tggatgataa agagttagaa gaatttttaa agaaagtccc tgaaaatatt
     2341 ataactatat tggatgaagc atatatagaa tttttaacag ttcctaatta tgtggatgga
     2401 cttaagtata taagggaaaa ttataacgtt atagtaacaa gaactttctc taaaatatat
     2461 ggtcttgcag ggcttagagt aggatatggt attgctaaag atgagataat aaggacttta
     2521 tttacagtaa aagagccttt ctctgcaaat cgtgttgcta taagtggagc aacagcagca
     2581 cttgatgatg aagagtttat aaaagaaaca tatgaattaa atagagttgg aatggcttac
     2641 tttaaagaag agtttacaaa aatgggattt gatgttgtag attcacaatc aaatttcctt
     2701 tatgtagata tgaaaacaga tatacctaag ttatttgaag acttgaaaaa aagagggttt
     2761 gtaataagac caagtgctac acatgcacgt gtaagtattg gaactatgga agaaaataag
     2821 aaatttgtag cagttttaaa ggaaatatta aatattaaag gagagccaag tattggataa
     2881 tagatagaaa tattatgaaa cggggtgtag atatgaactt aaaaataaac gcagagagat
     2941 gtaaaagttg tgaatactgc gttatatctt gtaaaaaagg agcattaaaa atatcaagta
     3001 agataaataa agaaggttat gctcatgtag aagttgatga gagtaaatgc atactttgtg
     3061 gaatttgcta tcaggtgtgt ccagataatg tttttgaaat aattaaagaa gaagcatgtc
     3121 aagaggctta gaaggagttg ggataatatg aaaaagatgt ggaaggggaa ccacgccatt
     3181 gcagaagcag ccctaagagg aggttgtgaa ttttatgcag gatatccaat tacaccacaa
     3241 acagaggtaa tggaattttt atcacataga atgtctgaac ttggaagaac ttttatacag
     3301 tccgaaaatg agatggctgc tatatatatg gtatatggag catatgcaag tggaatgagg
     3361 tctatgacga gttcttcagg accaggaata tctttaaaac aagaagggat ttcttactta
     3421 tgtgcaaatc attacccttg tgtaatcgta aatgtacaga gatgggggcc tggtcttggc
     3481 tcattggact cagcacagac agactatcta agagatacta gagggggagg aaatggagat
     3541 tatcatttga tagtttatgc accaaacagt attcaagaga ctgtagattt gatgtataac
     3601 tcatttgatg tagcggagaa gtacagagtg cctgtcgaaa ttttgacaga agcagcttta
     3661 ggtcaaatga tggaaccagt agaatttcca gagtttaaga agagagaagg agatttaggt
     3721 tggacttatg atggaagtaa tagagaccat gcaaaggttg cagataacca aaagccaact
     3781 ttttgtatgg agaaacgtat gagaataact gaaaatgaac aacaatggga agcttatcaa
     3841 gtagaagatg cagaatatgt gtttgtagcg ttcggtatac catcaagaac aactatgaat
     3901 gctgttagaa gacttagaga acaaggtgaa aaagttggaa taataagacc aataacagta
     3961 tggcctttcc catataaagc atttgaaaag gtaagtaaag atgtaaaggg atttatctca
     4021 gtagaatcaa cagacacagg tcaattagta gaagatgttg ctttagcctc aaagaaagta
     4081 tgtaaagaaa atgtacctgt gtatggttta tttagtggaa atcatatacc aaaaacattg
     4141 caagtaatgg acacttatag caaaataaaa tctggcgaaa tcaaggaggt gttctaatat
     4201 ggctacagaa aaattaacac ctagcgttgt ttcaatacca aacaagtttt gcccaggttg
     4261 tggtcatgga atagtaaatc gtataatagc agaggtaata gaagaacatg gatatgaaaa
     4321 aaatcatgta ttaacattag gagttggttg tgtctgcaat atgaatttta gctggaatgg
     4381 tgataagatg caaactgcac atggtagagc ttcatctaca gctataggag ttaaagtagc
     4441 acttccagat acattagtaa tgacttacca aggtgatgga gatgcttatg ttataggtct
     4501 ttctgaaact ttaaatacag catatagaaa tcataatgtg actgtatttg taataaacaa
     4561 taataatttt gcaatgactg gtggacaaat gtcatggacc actatgccgg gtcaagtcac
     4621 aacaacaagt gtaaatggaa gagatataaa gactactggt agtccactga aagttcctga
     4681 gatggtggca aacttctttg atgtagcata tgtagcaaga ggttcagttc atagcccaaa
     4741 ggaaataatt aatctaaaaa aatatataaa aaatgcaata gaagctcaat taaatggaga
     4801 gggatattcc ttagttgaaa tattagcacc ttgcccaact aactggggtg tatcactaga
     4861 gaaatctatc aaatggatgg aagaagaaat agtgccatat tatgctcttg gcgaatttaa
     4921 gcaaagggat ggtgaatagt tatggaaaaa gaactagtat ttgcagggtt tggtggtcaa
     4981 ggagttttaa ctcttggtct gattgtagct gagtcagcac ttgaattggg gaaacaagta
     5041 acttggatgc ctgcatatgg tccaactatg agaggtggaa aagcctatag tgtagtaaag
     5101 ttttctgatg agagtatagg tgggcctgat atggaagaaa tagatgtttt agtagcaatg
     5161 aataaaccat ctttagatta tataaattta gttaaagaag gtggaacagt agttattaat
     5221 acatctgcaa tagatgagaa tgtaacatta agagaagata taagtgtagt taaaataaat
     5281 tgtcaggaat tagctcaaaa agtaaacaat ccaaaagcag caaatatagt agtacttgga
     5341 gcattaattg ctaaaactgg gttatttgaa aaagaattag ctttaaaaac tatgtgtgat
     5401 ttctttgagg aaaaaggaaa aggaaagttt aatgttcaaa atgaagcagc attcatggaa
     5461 ggctataata acgccatttg ataaattacc cttatcttaa gtgatttatc tcaagattta
     5521 ataatcttta gtgccaagag aaacttcatc ttgagataaa attcacttaa aatcacaact
     5581 ataaacatat aggagcgtga atttatgagt ttggaaaaac ttattagacc aaaaagtatt
     5641 gctatagtag gagtgactga taaacttggt tttggaagaa gtgcagcact tagtatagta
     5701 aaaagtaagg aaacagatag agtatattat gttaatccca aaagagaaga attgtttggg
     5761 agaaaatgtt ataagacgat acaagaagtt ccagaagtag tagactgtgt agtagtatgt
     5821 acgcctagaa atgtagttcc atctgtatta aaagattctg gagagttagg aacaaaggca
     5881 gcagtggtat atgctagtgg atttgcagag gaaggtacag aggaaggtac agatttagaa
     5941 aatcaactta tagaaatatc aaatacatac gatatgaaaa tattgggtcc aaactgcatg
     6001 gggttgctaa attgtataga taaggttaat ctatgggcag gaggttctaa atgggattta
     6061 gatactaaaa aacctggtat aggaattgta ggtcaaagtg gatttataac tgctgaaatt
     6121 gtttcaagtg attattttaa tatatcttat ggtttttcaa caggaaatgg aaatatagtt
     6181 acactagaag atgcagtaga tttccttgta gatgatgatt atgcatcagt aatagcaatt
     6241 taccttgaag gtttaaaaaa tcctcaaaaa tttatagatg ctttaaaaag ggcagcacaa
     6301 aaaagaaagc cagtaataat attgaaatca ggaaggtcag aaaaaggtgc tatttctgct
     6361 gcttctcata caggaaatct agctggttcg agtaaagctt ttgaaagtat ttttgaaaaa
     6421 tatggagtaa tttctgtgga aaatttagaa cagtttatgt gtttagcaca agcttttagt
     6481 gttttagatg gtaatttacc tgcaaattct aattttgcag caataaactt ctcaggggga
     6541 gaaaatacta tttgtgcaga tttagcagaa gaaaatggtg tagaacttgc agaaatatct
     6601 tctgaaacta aagaagaaat gaaaaaatat cttccaggat ttgcaacacc taaaaatcca
     6661 ctagatgcta caacagcatt attccatgaa aaggatatga tagtaggttt attacatacc
     6721 tttgaaaagg atgatagtgt tggatttact atggtaggtg caaatataag agatgaagaa
     6781 aatgaaatgc atgaaaccct ttgtaaagct gtaagtgaag ctagagaaca aggattgaaa
     6841 aaacctgtgt ttgcagttcc tacacttgaa ggaaatagat atttagatta taggactaga
     6901 ttagaagata atcaagtgcc tataatgtca tcagttggaa ctacatttac ttgttttaat
     6961 aaaatggcta agtttataga ttatgattat tcaaaaagaa ctttagaatt taaagctgtt
     7021 aagaaaagag agtcaaataa tgtagtagca ttatcagaat tagatagtaa gattgagatg
     7081 aaaaaatatg gagtaccagt tccaggacaa ggtaatgcaa aaagtattga tgaattagat
     7141 gaaatcttaa aatatattaa gtatcctgtt gttttaaaaa taaattcttc tgaaatattg
     7201 cacaaatctg atgttggagg agttaaaata ggaattaaga atagagatga ggcagtagat
     7261 gcatataatg agattttaac taatgtaaaa aaagcaaaac cagatgctaa tattgatggt
     7321 atattagttc aagaaatggt agaaagtggt atagaaatta ttattggtat aactaatgat
     7381 gaccaatttg gaccaatgtt attagtcggt ttaggaggag tatttgtaga ggtatttaaa
     7441 gatactacat tatatccatt acctataaat catgatgaag ctattatgat gcttaaaaaa
     7501 ctaaaatcat ttaagctttt aaatggctat agaggaagtg agccttgcga tattgatgct
     7561 cttgcagata tgatggtcaa attaggaaaa tatgcttatg aaaataagga tgaagtcaag
     7621 gaaatagact taaatccagt gtttgtatat cctaaaggaa aaggcgtatg tgcagtagat
     7681 gccttaatag ttaaatataa atagtttata agttttggta ttcaaccata attaaagcac
     7741 cgactgaaaa tcacattttg taagttagat agtgtgattt tcagtcaccc caaatttaat
     7801 ttaaaaatat tttaaaatga aagtatagtt ttaagtttca ttgtgaaata gcactgtata
     7861 aacaataaaa tactacgtat aaaacgaata ataaaaggtg ttttaatgat aagaatcaat
     7921 cttgattttc attaaaacac cttctttatt atattaaaaa tttttaaatg ttatattgtc
     7981 acaggtgggt ttgttggagc tgcatatgta cgattagcaa attcttggtc aacaaagaat
     8041 agaccaccct ctccagcaag tttaacacgt tttatattgt cttcacaatt tgtttcttct
     8101 tctgcttgtt cagatataaa ccaaagtaag aattgagaag ttctaaagtc tgattcagag
     8161 tttgctacag ctgctaagtt gtttattaaa gctgtaacct ttttttcatg agctaacgta
     8221 cgctcaagaa tatccattgc agaagtaaag tctgaatctg gcattgggat agcatcaagc
     8281 tgaacctttc ctcctacatt atgtaagtat ttgtaaaaaa acatagcgtg gtctgtctct
     8341 tctttatatt gaacataaaa ccaattagaa aatcctttaa gtccttctgc ttcaaggtaa
     8401 gaagacatag aaagataaag atacgcagaa tataattcat ggttaatctg ctcattaagt
     8461 aatttttcca ttttagctga aatcattttt atacctccaa attttaaatg ctcttataaa
     8521 atataataat attaaatgga aaaatattca ataaaagtgt ttaaatattt tacatattta
     8581 ataaaaattt atactaccat taaaaaaatt ataacttaca atggctatag agccaattaa
     8641 tggtgcattt gctccaaaat ttgaatgcct tacacttatt tttttatatt tagaaaaact
     8701 tgctgaagca gtcaattttt ttaataatgt attttctata aatgtaccag gtatattaaa
     8761 gtcatagcct attattatag aactacaatc aatcaatgta agtgtattaa ctaatgcata
     8821 tgatacgtat gtacaaaatt catcaagcaa aaacatggca agactatctt tattatttgc
     8881 ctcatctatt aacttgacta aagaaaaatc tttacagtta attaatgggg agttaggata
     8941 aatatggctc aatgattcaa ttcttttaat tagattactg acacttgtat aataatctaa
     9001 acatccattg ttaccacaac tgcacaatgg tccactaaaa ttaattgatg tatgtcctat
     9061 ttctccactt tgtcctaggt tgccagtatg aagtttattt tctagaacta gaccagctcc
     9121 aataccattc attatgtgta ggtatatata atttgatata tttttaccta aaccatacat
     9181 tttttctgct aatgcacccg aattagcatc atttatcaaa aatattggca aatcagacat
     9241 ttcttttata aaatctacta tatttaggtt tgatacattt ccaaaatctg ggggatttag
     9301 tatggtacca tttatatcat ctactggacc tattgaagaa ataccaattc caattatatc
     9361 tctcttgttg attttcatta ttttagtaaa cattcttttt attatatctt ttaaaacatc
     9421 atttgataga aatttagggt aagtttcaga aagttgtttt acaatattac cttttaaatc
     9481 tgaaataatt accttacata tatttcttct tattaggatt ccacaaatac aaggcgatat
     9541 attagaaata tctaataaaa tgggtttacg acctgatgaa tgtgcattta aagttgatga
     9601 ctctatttcc ctaatcatat tttcattaat aagttcagta actagatttg ataaagtcat
     9661 tttagtaagt cctgtagact tagctaaatc aactctggac ataggtgtat ttgtagaaag
     9721 taatttcaat attattaact tatttttaat ttttacatct ttgatgttag aaccttgttt
     9781 gttactcatc ataaatctcc ttaaataaag ttaagtgttt tatttatttt caagtatact
     9841 atcaccttat aatagagtaa cattttttta cttgaaaata tcattaatta gaatatatca
     9901 cattatatat cataaaatat ataaaaaaat gttagaatta aattaaaagt gaaaagtaga
     9961 ttgatatata aatagtaaat acttaattaa aaaaagataa tgatagcaat aattattgtt
    10021 accaagaatc tactacttca agtgttacct taggtattta agttgatagt ggcttgcacg
    10081 taataaactt gatgtaatat atattcttaa taatatcgat aaaagattta cttaaaaata
    10141 atatagatta tgtaaacata taatctattt attgtataca ataaaatttc attttgtaaa
    10201 acagtgaatt aaatatgtaa tatagatatc gattttta
//
LOCUS       DAEMHW010000137        10035 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEMHW010000137 DAEMHW010000000
VERSION     DAEMHW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10035
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.137"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            283..1914
                     /locus_tag="KPK67_002760"
     CDS             283..1914
                     /locus_tag="KPK67_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2459702.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(2049..2975)
                     /gene="fba"
                     /locus_tag="KPK67_002761"
     CDS             complement(2049..2975)
                     /gene="fba"
                     /locus_tag="KPK67_002761"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBG2459703.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            3305..3634
                     /locus_tag="KPK67_002762"
     CDS             3305..3634
                     /locus_tag="KPK67_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBG2459704.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            3634..4356
                     /locus_tag="KPK67_002763"
     CDS             3634..4356
                     /locus_tag="KPK67_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG2459705.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            4383..5744
                     /gene="rlmD"
                     /locus_tag="KPK67_002764"
     CDS             4383..5744
                     /gene="rlmD"
                     /locus_tag="KPK67_002764"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG2459706.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKSELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            6266..7300
                     /locus_tag="KPK67_002765"
     CDS             6266..7300
                     /locus_tag="KPK67_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBG2459707.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            7387..7590
                     /locus_tag="KPK67_002766"
     CDS             7387..7590
                     /locus_tag="KPK67_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBG2459708.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            7761..8921
                     /locus_tag="KPK67_002767"
     CDS             7761..8921
                     /locus_tag="KPK67_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBG2459709.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFVTDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSKNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            9181..9321
                     /locus_tag="KPK67_002768"
     CDS             9181..9321
                     /locus_tag="KPK67_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459710.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT         4005 a         1065 c         1788 g         3177 t
ORIGIN      
        1 gattatcttt gtttttttaa ataaatctta aatttaatta aattgtatta aatagacaaa
       61 taattactaa atatgtgata aaatacaatt aaaagcaaaa tgaaattttt aacaaactat
      121 aatcattaaa attatatctt agttacgttt taatgaaata aactacttga aaagacgaat
      181 attataagga gttgattata tgtgctgatg gtatttattg taagcaaaca ataaattttg
      241 tatgtagttc aataaattac ttaatgaaag gtgcgaatat taatggataa taatttaata
      301 gggcttatag aacatgtaga aaatccagcc attttatgta aagaatcagg cgaaataata
      361 tactgtaatc atctaataga tagtattttt agttttctag atataaaaaa gcctagaaat
      421 ataaatgaat tagattcaaa ttttgacaaa acagaaatac tgacagatag taaaaagaaa
      481 atagctttca gagagttaag gatgactgca catatataca atatgaaaga taataataat
      541 gaaaataata tagtttattt atttgagaag tctctaatat ctgataaagt aattgaagat
      601 ataatagagc atatagatga agttgttgtt gtgtttaaca aagatggggt aattgaaaag
      661 atgaacactg tcagtgatga gatattacct tttaaaagaa ctgaggtact tggtagaaat
      721 ataacagacc ttgtgagaca aggattagtt gaagaaccaa taatattaaa catgcttaaa
      781 gtgaagaaaa agatttatag aaatatcgtt tacccagatg ggaagcttat tgcatatacg
      841 gcagttccaa gatgggattc taagggaaag cttacaggag gagtactaac agggagagat
      901 atttcaaggg ttattaagct tgaatctcag ataaaatata gtgatatatc agaagacaca
      961 gagtatataa gtcaaagtaa gattatggac aatataaaaa aagttgttaa gagagctgca
     1021 gcctcagatt cttctatatt tataaatgga gaatcaggtg taggaaaaga aataatagca
     1081 agaacaatat ataagtatag ttcaaggaga gacaaacctt ttatagctat aaactgtgga
     1141 gctataccaa atgaattatt agagtcagag ttttttggat atgaagaagg ttcttttact
     1201 ggagctaaga aaaaaggtaa aaagggactt tttgaagagg cgaatggtgg aactattttc
     1261 ttggatgaga taggtgagtt accaatgcag atgcaaaaaa aattacttag ggttatacaa
     1321 gaaaatacta ttactagaat aggtggtagt aagcctataa aaatagatgt tagatatata
     1381 agtgctacta atatttctca tgaagacctt agaaataacc ttaaatttag acaagatttg
     1441 tactatagat taagtgttat tccagtaaaa atacctcctc ttagagaaag aaaagaggat
     1501 atagtaccat tagtaaatta tttcttaaaa ctttacaatg aaaagtacaa tagagaagta
     1561 gaagtttcgc ctaaagttat tgagttatta gaagaatatt cttggccagg aaacataaga
     1621 gaattaaaaa acatcattga aagatttgta gtattatcag ctaaaaatgt aattggagaa
     1681 gatgaattta acatgctgat aaatttagat atgattgata atgaaacaga tgatttatca
     1741 ccaatagtgg taaatggaat tatgaatctt aatgatgcat ataaaatagt ggaccaaata
     1801 atgataagta aggctataaa taagtatggt tcaataacaa aggctgctga agttataggt
     1861 atatatcctt ctaccatcca tagaaagata aagagtggac atattcacgt ttagctgtca
     1921 gtttaaaaag acaatataat taaaatatta taagatacat aattgataca acttaaaaga
     1981 gatggcttaa aaataaattt aattacttta agtcatctct tttcttttat aaatatttaa
     2041 aagttatatt agtttataga gttagcagaa cctaaaacat catctatctt gctttcaact
     2101 acagcttgta tagcatctct accagcacca atgtattttc ttgggtcaaa ttcttttgga
     2161 ttttcagcta agaattttct gatagcagca gtcatagcta gtcttaagtc agtatccatg
     2221 tttattttac aaacagccat agaagatgct tttcttaaca tatctacagg tacaccttta
     2281 gcaccagcta tatttccacc aaattcatta catgtagcaa cagcgttttg gtcaacagca
     2341 gatgcaccat gaagaactat tgggaaacct ggtaatttgc tttggatttc ttctagtata
     2401 tcgaatctta atttagcttc tcctttgaat ttgaaagcac catgagaagt tccaatagct
     2461 attgctaatg aatcaacacc tgttctttca acgaactcaa ctgcctcagc tggttgagta
     2521 tacttgtgaa catcagaagt aacatcatct tctgttccag ctagaacacc aagttctgct
     2581 tcaacaacaa cacctttaga gtgtgcatat tcaacagctt ctttagttat tctaacattt
     2641 tcttcaaagt caaaatgaga accatctatc ataactgaag agaaaccagc atcaatacaa
     2701 gttttaatag catccatatt tggaccatgg tctaagtgaa gagctacatc tacacctatt
     2761 tcatcagaag cagccttaac catttcaact aatgtatgag gtccagcata tttaacagct
     2821 gacatagaag cttgtatcat aacataagaa tttttagctt tagcagcttt taaaacacct
     2881 tgtaattgtt ctaagtcact tatgttaaat gcacctatag caaatccacc ttcgtaagct
     2941 tttttaaaca tttcttttgt agtaattaat gccattttaa actcctccta ttaactttta
     3001 aaatttaccc atcacttata ttataacgtt aaatcaaaaa aagtatatag taaattgtaa
     3061 taactataaa ttacaagtat aatgttaaat agaagacaat aggattaaat aaaataataa
     3121 agaatataaa caatttagga aaattagcta tataatgcta actataaggg taggaactac
     3181 cttattagtt tggagatact gataacaata gttgtcttga ccaagaagtt accacttcaa
     3241 acgttatcga agggaattaa gtggatgata attcactaaa aatttaaaat aaaggagata
     3301 aactatgcca cagataaaaa taagaggaat taatgaaaat gatatatgta aaataagtga
     3361 aaaaatgata aatgatttag ttgaagctgt aaaatgtcca agagactact ttgaaataga
     3421 atgtataaag tcagttgcaa taagagatgg taaaatagca gatgtatatc cttttgttga
     3481 agtagcttgg tttgatagag gacaagaagt acaagatata gtagcaagaa taataactga
     3541 tagtataaga aataatttag atgtagaaag tatggattta gcatttacag tatttgaaaa
     3601 agaaaaatat tatgagaatg gagagcattt ttaatggcta aaaagcaacg tatagataaa
     3661 attctttcaa atttaggtta tggaagtaga agtgaaataa agaagtattg taaacaaggg
     3721 tctgtagtag taaatggaag tgaagtttca aatccaggaa ctcaagtaga cactgaaaat
     3781 gatgagatat tatttaatgg agaagaagtt atatatagag aatatatata tttgatgatg
     3841 aataaacctg atggatatat atcagcaact acagataaat atgacccaac agtattagat
     3901 ttaattgatt tatcatactt agcttttgaa ccatttcctg ttggaaggtt agataaagac
     3961 acagagggat tattagtttt aactaatgat ggaaagcttt ctcatagagt tttatctcct
     4021 aagaagcatg tgcctaagac ttattatgca aaaattgatg gagtagttac tgaagaagat
     4081 gtagaagctt ttttagaagg tgtagtgctt gatgacggat ataaaactat gccatcacag
     4141 ttaaatatat taaaaagtga tgatgaatca gaaatagagc ttacaataca tgaaggtaag
     4201 tttcaccaag taaaaagaat gtttgaaagc gtaggcaaaa aagtagtata cttaaaaaga
     4261 ttgtctatgg gcaatttaaa attggatgaa agtttagagt taggtgagta tagagagtta
     4321 acagatgaag aagtaaaatt gattgaagaa agataataaa gatactatag taaattggag
     4381 gaatgtctgt gaagaaaaaa gatgttatag aatttgaagt agataaaatg gaatttggtg
     4441 gtacatcttt aagccaagtt ggagatagag ttatccatat gaagggtgga ataagtggtc
     4501 aaaaggtgag agctggagtt aaaaaagtta gaagtaagaa agctgaagtt aagatgatag
     4561 aattattaga gaactcacca cttgaaactg aagaaacttg caagcacttt agagagtgtg
     4621 gaggatgtac actattgtct gtgccatatg aaaaacaatt agaaataaaa gaaaaacaag
     4681 ttatggattt atttttaaag caagatttat ttggatttca gtttttaggg atagaaggaa
     4741 gtccagaaaa caaatattat agaaataaga tggaatatac ttttggagat gaagtaaaaa
     4801 atggaccttt gacattaggt cttcataaaa aaggaaagca tatagatata caaactgttg
     4861 aggagtgtat gttggttgat gaagacttct caaagatatt agtttctagt gtagagtttt
     4921 tcaatgaaaa aaagcttcct tattatagaa caatgaatca caaaggatat ttgagacatc
     4981 ttgtagttag aaaagggatt cacactaatg agattatggt taatatagta acttcttctc
     5041 aagaagattt tgatatgaat gagtttaaag atatgctttt agggattgat ttaaaatctg
     5101 agttggtggg ggtacttcat actattaatg atggtttagc agatgcagtc caatgtgatg
     5161 agttaagagt attgtatgga agagattata tacaagaaga aattttaggt cttaagttta
     5221 agatttcacc attttcgttt ttccaaacca atactaaggg agctgaggtt ctttatagta
     5281 ttgctaggga ttttattgga gattacaatg ataaagttgt atttgacttg tatagtggta
     5341 ctgggtctat tggacaggtt atggctggtg ctgctaagaa agtgtatggt attgagatag
     5401 ttgaggaagc tgttgttgct gctaatgaga atgctaagtt gaatggactt actaattgtg
     5461 agtttattgc tggggatgtg gctaaggttg ttaaggattt aaaggacaag cctgatttga
     5521 ttattgttga ccctcctaga ccaggcattc ataaggatgc tattagggat atttgtggat
     5581 ttggtgctaa ggagattgtt tatatttctt gtaatcctaa gtctttggtt gttgatttgg
     5641 ttgattttaa ggggtatggt tatgagatta agatggttaa gtgtatggat atgttcccaa
     5701 atacgccaca ttgtgagact gtagttaaac ttattagaga ataacagggt ttcagacttt
     5761 tatagtgtgt cttaaaagta gatttgccac ctatttgcca ccataattat ttttgggtgg
     5821 caaatttatt ctacaatgat ttcaaaaata tttaccgtct ggtttctcat gttattagtt
     5881 acatgtaagt atgtatccat agttgttgaa gttttagcat gtccgagtat atgctggata
     5941 tcttttatat tagtaccatt ttctaataac atagtatcag ggtttttaaa ataaagtgtg
     6001 tattcttcta aagtagtgat aattaaaata tcaaatatca ctatacaaag aaaagtgcac
     6061 gctttttatg tttaacaaaa acgaaatatt aaaaaactta ttgaagaata tgatgtcaaa
     6121 acaactacag atattcaaga tatgctaaaa gatttattta caaatacaat tcatcaaata
     6181 tttacattgt cgaattttaa cggtttaaac ataatatatt gactgatata ataaaatcat
     6241 agaatatttt agggggaaat agattatgtt taagaaaaag ttaatagctg ttgcaatgag
     6301 tataactatg attagtgtag ggagcttttc gtatgctcat tcaggaagaa ctgattcatc
     6361 aggaggtcat cgtgataata aaaataaatc tggattagga agttatcact atcattgtgg
     6421 tggttatcca gctcatttac ataataatgg tggttgtcct tatactggag gaggaagcag
     6481 ttctggaaca acaacttcgg ttaataatga agaaaagcaa aagaggtctg ttggagaaaa
     6541 aggatataat caagggtatg aagatgggta taaaggaaat tatagttcat caaattatag
     6601 tggagattat agtgatacat atgaatcaaa gtactcagaa ggatatgaaa aaggtaaggc
     6661 taaattagaa gaggaagaaa aggtggctaa agagactgga tataatttag ggcttacagg
     6721 tgctaaatct aataatacat atgaaaaaga agttttaaaa aatgcttatg ataccggata
     6781 ttcaactgga tacaatgaat ataagactaa aaagatagaa gaatataaag ctaaagggat
     6841 agaggatagc aataaagata aagaaaaaat gacatttgaa gaaaacatag attctgaatt
     6901 tatagatgct tataataatg cttatgacga aatacaagaa caattaaaaa atgattacac
     6961 tactcaagga tttgagagtg ctatcaaagg agaaagattt gacacatcaa ctataggtaa
     7021 tgttaaatat gctaattggt ttaaagaagg atatgataat ggtaaggtta agttacctaa
     7081 agcaaaagaa agtgtatata atcaaggtta caatgaagaa gattttagta taccagatga
     7141 aatgaagtct attgaaacta agttaaaagg tgtatatgat gaaggtttag aaaaaagaga
     7201 agaagagaaa agtagaaatg ctacttatgg ggttggagct ggaacagcag ctgtagttgc
     7261 tggagtagta tataagaaga aaaaacgtaa aaagatatag atgctagata taccaatgat
     7321 gacaagtgta taacaaatat aattttaagt ataaatattt aaaatataga agggaaggtt
     7381 atatctatgg gatttaactt taggaaaagc ataaatctag gaggaggact caaattaaat
     7441 ttaggtaaga agagtgtagg tataagtgct ggtgttaaag gtgctagagt gtcagttaat
     7501 tcaaaaggaa gaaaatcagc tacattgagt atacctggaa ctggaatatc atatacaaaa
     7561 actagtacaa agaaacgtaa acctaaataa atatttgata tactaagaaa tagactgtta
     7621 caaatagtct attttttagt atatagattt aaaaagtgac aaaattttga aatataaact
     7681 aatttcataa aggaaaatat tcttttatgt agaattataa tatttaagta atattataaa
     7741 atttatagga ggatttcatt atgaaattaa gcaaaacact attggcaata gggatgtctg
     7801 tttcattatt gattgctagt tgtccaacat caaacgctct tagttctatt aattcaataa
     7861 aaggtacaaa taagtatgaa acagcaggtt taatagcaga taaacaaaat tatgatattg
     7921 caatacttat taattctgaa aatggattat ctgatggatt gtcagcaagt ggattgtcag
     7981 gagctaaaaa tgccccaata ttgttaacta agaaagatac aatcccttca gaaacattaa
     8041 aaagattaga aaaggtaaaa gaagtatatc taatcggagg aaaaaattcg ataggagcat
     8101 cagttcagaa aatattaagt gataaaaaaa taagcataaa tagaataagt ggaactgata
     8161 gaatagatac aagtcataaa gttgcaaaag aaataggaag agtaagtacg gttaaaaaag
     8221 taatattaac taatgctttt aaaggcgaac cagatgctat gagtgttgct tctatagcag
     8281 ttagagataa atctccaata gtattaactg atggaaatac tacaagtttt gttacagatg
     8341 gattagaaac ttatgcaatt ggtggaaaag tcagtatgag tgataagtta ataaatgaaa
     8401 ctaaagcaac aagaataggt ggagtagata gatacgatac aaataaacaa ataataaata
     8461 aattttataa tggctcaaaa gaattttaca tagcaagtgg aactgattta gtttattctt
     8521 tagttggttc tacaattgca caaaaaacac ctatagtgtt agttggaaat ggaagtaata
     8581 agagtatatt aaaaaatgca agtaaaataa ctgctattgg aaatattgat gatgttgtta
     8641 taaaacaatg tttaaatata actaataatg ttggggattc aaatacaggt attgtaaaag
     8701 atactaattc aaagaatacg aacaaaccaa gtaatgagcc tagctctaat aatcctggat
     8761 taactgataa aagtacagta tatgtaactc ctaatggaaa atcatatcat ctaacaaaaa
     8821 attgttctac actaaaaaga agtaaaacaa taaataaagt aacattaaaa caagctaagt
     8881 cacaaggtaa aatagaccct tgtaatgttt gtgtaaaata atgtattagc caaatatata
     8941 ttaaaaaaga caattaatta agattgtctt ttttattgaa gaaatataaa tttatattat
     9001 gtattattaa aaagaaaatt attaattata aaaaataata ataaagacga taagtattaa
     9061 aattttatgt ttccaatatt tatgatataa ttaatatggc aaggaataaa tatttgaaaa
     9121 agctatgagt ggtgtttcca tggaagtttc ttcactttct tatgagagga ggtgaaaact
     9181 atggaaaatc taatgatgag tattataact ggtgttatag ccagttacat ttacgataaa
     9241 ttaaagagcc actctgagcg accaactaag agtggctggg aacttaatat aaagttccag
     9301 aaaagtaaat cttcaaaata atttgttgaa ggaaacatca ctcaaagtta agtaaaatat
     9361 gtttccttgc ttttattata tcacaaatta aaaatattaa cacaaaattt tgtgaatata
     9421 tacttattat tgttctaaaa aaatatgtaa ataattgatt tgattgcata gaattattga
     9481 tgtaatattt aagttaaata tacacaatct caattaaaga agataagtac tataaattca
     9541 tatcaaaaaa atacataaac ttttaagtta taattttata aaaagtcttt tagtatcatt
     9601 atttcaagag gaattagcat atgaaaaaat aacttcatta gaaaagtgga taaaaaactg
     9661 gtaagaagat gactaaagat tacatatact attacttaaa ttaaaaattt aatataattt
     9721 ataatgtata caatttatta taaaaaattt aattttaatt ttctattttt gatactataa
     9781 tatataatct tgataattta attaaaaatc tagattttac ataccaaact ggtttcttat
     9841 ttcgctatct ttacttatga actcatatat tcctataaag gttattttag aaaaagttgg
     9901 ttatctaaac attaatacca caatttctat agattgtttg gtgaataatc tataattata
     9961 ttatggacta aaaaatccaa tgagctaatt tattgttttt ttatatttga gagattacaa
    10021 cttattagtt tgtat
//
LOCUS       DAEMHW010000138         9833 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEMHW010000138 DAEMHW010000000
VERSION     DAEMHW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.138"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            84..359
                     /gene="flgM"
                     /locus_tag="KPK67_002769"
     CDS             84..359
                     /gene="flgM"
                     /locus_tag="KPK67_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBG2459711.1"
                     /translation="MNIKSVSSNIVVNNYLKNKSDVKKVQQVQKVDKTDKIELSKFAK
                     EMSSVSNMSDEAARNKKIEEIKSALENGTYKIKPEEIAKKMIEEMRG"
     gene            364..780
                     /locus_tag="KPK67_002770"
     CDS             364..780
                     /locus_tag="KPK67_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBG2459712.1"
                     /translation="MITDLKVIIYEEKKIISNMMELLKEQFDYIIEKDLENLNRLNPK
                     LEDISRELASIEIKRRQLLGDDVSISEVVENSNDEYLKEIYIDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPVDKQTTTYNSYGNIRK"
     gene            798..2108
                     /gene="flgK"
                     /locus_tag="KPK67_002771"
     CDS             798..2108
                     /gene="flgK"
                     /locus_tag="KPK67_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBG2459713.1"
                     /translation="MAGLFSILNTANSGMNAHQKSIQTISHNISNLDTDGYSRQRTGF
                     ATNSPMYMPSLSNSIGRGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKGDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDLENSDVKNMLSDGKLELNELKNVDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNASAGITVRDFFITSNVAGEDIIKVNPAIKSNSNELKLTTEEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFVESIGLDSQKVNQDEVNQRKIINNVDNSR
                     MSVSGVSLDEEMTELMKVQRSYQASAKVMSTAVQLLDVVINGII"
     gene            2127..3059
                     /gene="flgL"
                     /locus_tag="KPK67_002772"
     CDS             2127..3059
                     /gene="flgL"
                     /locus_tag="KPK67_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBG2459714.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQINTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYIENCKDTADWLETTDTSLDQLGNLLADIKKGLVSSGNGSY
                     SDDEIKTISNSTNEKMKEIANALNATHEGKYIFSGSNTGTPPVECVENADGSVSLKFN
                     TSLNLNKLNDSLSVDVAQGISVDYNVKLSSLGFDPTKTDPFEKLNNISKKLANPSDAN
                     IKELTTTCLGDMENLIDNTVNVRSIYGTKANTVDAMKEKNDEGLIQLKDVLSQNDEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            3075..3467
                     /locus_tag="KPK67_002773"
     CDS             3075..3467
                     /locus_tag="KPK67_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBG2459715.1"
                     /translation="MMKITLKKGILGFENLKEYELLDIENEDILKEFNSTEEDCIGFI
                     VVSPFEIIKEYEIVLNQETIEKLEVKSPNDIMLLNIITVGQTLEESTVNMKAPIVINV
                     RNNCGMQIILQDEEYSIWHPLLRGDGGC"
     gene            3461..3673
                     /gene="csrA"
                     /locus_tag="KPK67_002774"
     CDS             3461..3673
                     /gene="csrA"
                     /locus_tag="KPK67_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBG2459716.1"
                     /translation="MLVISRKKDEAVLIGDNIEVKVVGVDGNNIKLAISAPNNISILR
                     KEIYEKVKNENIKATNKNIKILKSLK"
     gene            3695..4090
                     /gene="fliS"
                     /locus_tag="KPK67_002775"
     CDS             3695..4090
                     /gene="fliS"
                     /locus_tag="KPK67_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG2459717.1"
                     /translation="MYGENPYNSYKQNAIFMASKEQLLLMLVDGAVKYTKIARGAIID
                     KNTRKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDVQII
                     DNVLPVIEEIKDTWHEADRKSREERNRYK"
     gene            4090..4440
                     /gene="fliS"
                     /locus_tag="KPK67_002776"
     CDS             4090..4440
                     /gene="fliS"
                     /locus_tag="KPK67_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG2459718.1"
                     /translation="MELDLNELNGLSKEELLLMLVDGTVKYTNISKVALLNKDYLKAH
                     NELVRVQNIFTELMVTLDQSVGQWAKDMYKVYEFIKHELVKADINKDIKIIDDILPIV
                     EQIRDTWHEVYNKL"
     gene            4468..5991
                     /gene="fliD"
                     /locus_tag="KPK67_002777"
     CDS             4468..5991
                     /gene="fliD"
                     /locus_tag="KPK67_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBG2459719.1"
                     /translation="MSSISPVRVTGLSGNFDMEGIIEASMIRDKEKVNKAKQDQQIVK
                     WKQEIYRDIIKESKNLYDKYLNGDSPNSITNKKAYSATRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKVTIKLNSSDPIVQQFPPNASGASSLNIGGVNIPISEQDTTSTI
                     VSKINSLCADNDIRASYSEMTGELIISRKQTGSSSDIDLKVIGNDSLAGQIASDNGIT
                     FTTDASGTKSAVVYGKNLEADVTDDQGRVTHISKEQNSFKIDNIDYNVNSKGSAKLVS
                     VTDTEEATKNMKAFVDDYNALMDKVYGLVTTKKSKDYPPLTDEQKDDMTTEEIEKWEK
                     KAKEGILRNDDELRAFVEDIQSMFFGDADTIIALRKLGISEHENYNKKGQISFNADTF
                     SKALIDDSDKVYKALAGYSSNYDDKGMFEKLKKIVFEYSGSSASKLTKKAGMENSSSA
                     SQNVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNKYSSQMNYFSQAQGN"
     gene            5994..6329
                     /locus_tag="KPK67_002778"
     CDS             5994..6329
                     /locus_tag="KPK67_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBG2459720.1"
                     /translation="MDKLEEKINLYKDISLKIINFIEKMEYKNISFQLDERQNIINSI
                     SEVDKSEFIQLYDSMELFEIDAKIRDALQEQLSEVKKELHEYKLTKQVNTMYYSSNRE
                     KVNIFNKKV"
     gene            6430..7302
                     /gene="fliC"
                     /locus_tag="KPK67_002779"
     CDS             6430..7302
                     /gene="fliC"
                     /locus_tag="KPK67_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBG2459721.1"
                     /translation="MRVNTNVSALIANNQMGRNVNGQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGSLEETGNILQRMRTLSLQSANEIN
                     NTEEREKIADELTQLKDEIERISSSTEFNGKKLLDGTSSTIRLQVGASYGTNVSGTSN
                     NNNEIKIQLVNTASIMASAGITTTSIGSMKAGGTTGTDAAKTMVSSLDAALKSLNSSR
                     AKLGAQQNRLESTQNNLNNTLENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            7396..9535
                     /locus_tag="KPK67_002780"
                     /pseudo
     CDS             7396..9535
                     /locus_tag="KPK67_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4233 a          978 c         1709 g         2913 t
ORIGIN      
        1 ttaaaatcta tttgaatcgt ataaatattt ttcttctttg agcgataatt aaaatataag
       61 gatgtaattt tggaggtgag gttatgaata ttaaaagtgt aagttcaaat attgtagtaa
      121 ataattatct aaagaacaaa tcggatgtaa aaaaggtaca acaagttcaa aaagttgata
      181 aaacagacaa aatagaacta tctaaatttg caaaggaaat gtcaagtgta tcaaatatga
      241 gcgatgaagc ggctagaaat aaaaaaatag aagaaataaa aagtgcatta gaaaatggaa
      301 cttacaaaat taaaccagaa gaaattgcta aaaaaatgat agaggagatg cgaggataaa
      361 gtaatgatta cagacttaaa agtcataata tatgaagaaa aaaagataat tagtaatatg
      421 atggaattac taaaagaaca atttgattat ataattgaaa aagacttaga aaatttaaat
      481 aggttgaatc ctaaactaga agatattagt agagaacttg catctataga aataaaaaga
      541 agacaacttt taggggatga tgtttcaata agtgaggttg ttgaaaacag taatgatgaa
      601 tatttaaaag aaatatatat agacttaaag catattcttg ctttagctaa aaatcaacaa
      661 gaatcaaatg attctctaat aaagaaagaa cttattttca cgaaaaaaat gattaatttc
      721 ataaaaccag ttgataaaca aactacaact tataattctt atggaaacat tagaaaatag
      781 atattgggag gagaaatatg gcaggattat tttccatttt aaatacagct aattcaggaa
      841 tgaatgcgca ccaaaaatca atacagacaa tttctcataa tataagtaac ctagatacag
      901 atggttattc taggcaaagg accggatttg ctacaaatag tccaatgtat atgcctagtt
      961 tgagcaattc aattggaaga ggacaattag gtactggagt ccatgtaaca gatgtaacaa
     1021 gagctagaaa tagtttttat gattaccaat ttagggcaga agctcataag tatgggaata
     1081 cagtttcaaa atatgattat tataatacta tagaaactat actaaatgaa ccatcagact
     1141 atggaatatc tgcaggtata gatgattttt ttaaaggatg ggagtcatta tcaaaggata
     1201 taaatagtga tagtaaaaaa agtctagtgg tagaaaatgc atctaattta gcgactttaa
     1261 taagcgaaag ttataagaag cttgacaagt taaaaggaga tatagattct aatatagata
     1321 ctgaaataaa acaaataaat gacatgttaa aaagtcttga agacttaaat aaatctattg
     1381 atattatctc tggttctggc tctacaccaa atgatttgct tgatgaaaga gatagaatat
     1441 tggataactt gagttttaag ttagaccttg aaaatagtga tgttaaaaat atgctttcag
     1501 atggtaaatt ggaacttaat gagttgaaaa atgtagatgg aacttggaaa acaggtatat
     1561 caggaacttt acaaggcttg tttgaaatgc acggtaaaat tgatacatat aaaagcgact
     1621 taaaagatgt atctgatgga ttagcaaaac agataaatga tgtttataat gcatctgctg
     1681 gaataactgt aagagatttt tttataacct caaatgtagc tggtgaagat attataaaag
     1741 taaatccagc aataaaaagt aattcaaatg aattgaagct tactactgaa gaagcttcaa
     1801 agattgctaa gttaaaggat gaaaaaatag atattggggt agctggtgga aaagtaagca
     1861 caataagtga ccactacaag gcttttgtag aatcgatagg tttagattct caaaaagtta
     1921 atcaagatga agtgaatcaa cgtaaaatta taaataatgt tgataattca cgaatgagtg
     1981 tttcaggtgt atctttagat gaagaaatga cagaactcat gaaagtacaa cgttcctatc
     2041 aagctagtgc aaaagttatg tcaacagcag tacaactatt ggatgttgta ataaatggga
     2101 taatttaata aaaggaggaa tttataatga gagttagtac aggtatgatg tccagtagtt
     2161 atctaaattc attgcaagat aatttgcaaa gattagacaa ggtcaataga caaatcaata
     2221 caacgaaaga aataaataaa ctatcagata atccatataa ggcgataaag atattgaatt
     2281 caaaaagtga aataaaaact atggaaactt atattgaaaa ttgtaaagat acagcagatt
     2341 ggttagaaac aactgatact tcattagacc aacttggtaa tttattagca gatataaaga
     2401 agggtttggt atcaagtggg aatggttcat attcagatga cgaaataaaa actattagta
     2461 attcaaccaa tgaaaaaatg aaagaaatag caaatgcttt aaatgcaaca catgaaggta
     2521 agtacatatt ttcaggttca aatacaggca caccacctgt tgagtgtgta gagaatgctg
     2581 atggctcagt aagtttaaag tttaatacta gcttaaatct taataaatta aatgattctt
     2641 taagtgtaga tgtagctcaa ggaatcagtg tagattataa tgttaagttg agtagtcttg
     2701 ggtttgatcc aacaaaaact gatccatttg aaaagttaaa taatatatcg aagaaactgg
     2761 caaatccaag tgatgcaaat ataaaagagt tgacaacaac ttgtttaggt gacatggaga
     2821 atctaataga taatacagta aatgttaggt ctatatatgg tacaaaggca aacacagtag
     2881 atgccatgaa agaaaaaaat gatgaaggtt tgatacagtt aaaggatgtt ttatcgcaaa
     2941 atgatgaaat agattatggg gaaaagcttg ttcaattaaa ggctgcagaa ttaacttatc
     3001 aagcttcact acaaacagga ggaaagttat ttaatgtatc tatattagat tatatatagt
     3061 aaaataacgc agatatgatg aagattacat taaaaaaagg aatactagga tttgaaaact
     3121 taaaagagta tgagctttta gatatagaaa atgaagatat tttaaaagaa tttaattcta
     3181 ctgaagaaga ttgtatagga tttattgttg tatcaccatt tgaaattatt aaggaatatg
     3241 aaatagtcct aaatcaagaa acaatagaaa aacttgaagt aaaatctcca aatgatatta
     3301 tgttgcttaa tataatcact gttggtcaaa cacttgaaga atcaactgtg aacatgaaag
     3361 ctcctatagt aataaatgta agaaataatt gcggaatgca aataatttta caggatgagg
     3421 aatattcaat atggcatcca ttattgagag gtgatggtgg atgctagtaa tttcgagaaa
     3481 aaaagatgaa gcagtactta taggagataa catagaagta aaagttgtag gagtggatgg
     3541 aaataacata aaattagcta tatctgctcc taataatata agtatcttaa gaaaagaaat
     3601 atatgaaaaa gtaaaaaatg aaaatataaa agccacaaat aaaaatataa aaatcttaaa
     3661 atcattaaaa taaaacactt ggaggaagta atatatgtac ggagaaaatc cttataattc
     3721 ttataagcaa aatgctattt ttatggcgtc aaaagaacaa ttattgctta tgcttgttga
     3781 tggtgcagta aaatatacaa aaatagctag aggagctatt atagataaaa atacacgaaa
     3841 agcacataga gagcttataa gagttcaaga tatatttaca gaactaatgg ttacattaga
     3901 ccaaaatgca ggtcaatggg cgaaagatat gtatagagtt tatgattttg taagatatga
     3961 actttcaagg gcaaatatca gaaaagatgt acaaataata gataatgtat tacctgtaat
     4021 agaagaaatt aaagatacat ggcatgaggc tgatagaaaa agtagagaag aaagaaatag
     4081 gtataaatga tggagttaga tttaaatgaa ctaaatgggt tatcaaaaga agaattacta
     4141 ttaatgcttg tagatggtac agtcaagtat actaatatat ctaaagttgc acttttaaat
     4201 aaagactatt taaaagcaca caatgaactt gttagagttc aaaatatatt tacagaatta
     4261 atggtcacat tagaccaaag tgtaggacaa tgggcaaagg atatgtataa agtttatgaa
     4321 ttcataaaac atgaattagt aaaggctgat ataaataaag atattaaaat aattgatgat
     4381 atattgccaa tagtagaaca aataagagat acatggcatg aagtatataa taaactataa
     4441 ctataatcat gatgggggga taaacatatg tcaagtataa gtccagtaag agttacaggc
     4501 ctttcaggaa attttgatat ggaaggtata atagaagcta gtatgataag agataaggaa
     4561 aaagttaata aagcgaaaca agatcaacaa atagttaaat ggaaacaaga aatatacagg
     4621 gatattataa aagagtcaaa gaatctttat gacaagtatt taaatggaga ctctcctaat
     4681 agtataacaa ataaaaaagc atattctgct acaagaataa ctagttctga tgaaagtatt
     4741 atagtagcaa aaggctcagc tggtgcagaa aaaataaatt atcaatttgc agtttctcaa
     4801 atggctgaac cagcaaaagt aactattaag ttaaattcaa gtgaccctat tgttcaacag
     4861 tttcctccaa atgctagtgg agcaagttct ttaaatatag gtggtgtaaa tataccaata
     4921 tctgaacaag atactacaag tactattgta agtaagataa actccctttg tgcagataat
     4981 gatataagag cttcttatag tgagatgaca ggtgaattga ttatctcaag aaaacagact
     5041 ggttcatcat cagatattga tttaaaagta attggaaatg atagtttagc tggacaaatt
     5101 gccagtgata atggtattac atttacaact gatgctagtg gaactaaatc tgcagtagta
     5161 tatggaaaaa atttagaagc tgatgtaact gatgaccaag gaagagtaac acacataagt
     5221 aaagaacaga attcatttaa aatagataac attgattata atgtaaattc aaaaggaagt
     5281 gctaaactgg tttctgttac tgacactgaa gaagctacta aaaatatgaa agcatttgta
     5341 gatgattata atgcattgat ggataaggtt tatggattag ttactactaa aaaatcaaaa
     5401 gattatccac ctcttacaga tgaacaaaaa gatgatatga caactgaaga aatagaaaag
     5461 tgggaaaaga aggcaaagga aggaatactt agaaatgatg atgaattaag agcttttgtt
     5521 gaagatattc agtctatgtt ttttggagat gcagatacta ttattgcttt aagaaaactg
     5581 ggtattagtg agcatgagaa ttataacaaa aaaggacaaa tatcatttaa tgcagatact
     5641 ttttcgaagg ctcttataga tgatagtgac aaggtatata aagctttagc tggatattct
     5701 tcaaattatg atgataaggg tatgtttgaa aaattaaaaa aaattgtatt tgaatattct
     5761 ggaagttcag cttctaaact tactaaaaaa gcaggtatgg aaaattcttc gtctgctagt
     5821 caaaatgtat attcaaaaca aattgcagag caagaaagaa atataagtag gttagttgaa
     5881 aaaatgaatg ataaagagaa aagactttat gctaaatatt cagctttaga gtctttgttg
     5941 aataaatatt cttcacaaat gaactatttc tcacaagcac aaggtaatta attatggaca
     6001 agttagaaga aaaaataaat ttatataaag atatatcttt aaaaattata aattttattg
     6061 aaaaaatgga gtataaaaat ataagttttc aattagatga gagacaaaac attataaata
     6121 gtataagtga agttgataaa agtgaattta tacaacttta tgatagtatg gagctatttg
     6181 aaattgatgc taagataaga gatgctttgc aagaacaatt atcagaagta aaaaaagaat
     6241 tacatgagta caagcttact aaacaggtaa acacaatgta ttatagttca aatagagaaa
     6301 aagtaaatat ttttaataaa aaagtataaa gttatagatt aacttgtccg ataatataaa
     6361 cggatagtta atacaaaagt taaaagtaga aatgacaagg atgtcaacta tactaaggag
     6421 ggtaaaataa tgagagttaa tacaaatgta agtgctttga tagcaaacaa tcaaatggga
     6481 agaaacgtaa atggacaaag taagtctatg gagaagttat cttctggggt tagaatcaag
     6541 agagctgctg atgatgctgc tggacttgca atatctgaga aaatgagagc tcaaataaaa
     6601 ggtttagacc aagcaggaag aaacgttcaa gatggtatat ctgttgtaca aacagcagaa
     6661 ggttcgttag aagaaactgg aaatatacta caaagaatga gaactttatc attacaatct
     6721 gcaaatgaaa ttaataatac tgaagaaaga gaaaagatag ctgatgagtt aacacaatta
     6781 aaagatgaga ttgaaagaat atcaagttca acagagttta acggtaagaa gctacttgat
     6841 ggaacttctt caacaataag attacaagtt ggagcaagtt atggaactaa tgtttcaggt
     6901 acaagtaata ataacaatga gataaaaatt caattagtaa atactgcaag tataatggct
     6961 tcagctggta taacaactac ttcaatagga agtatgaaag caggtggaac tacaggaaca
     7021 gatgcagcta aaactatggt atctagctta gatgcagctc ttaagagctt aaactcatca
     7081 agagcaaaat taggagcaca acaaaacaga ctagaatcaa ctcaaaacaa tttaaataat
     7141 actttagaga atgttacagc agcagaatca agaataagag atacagatgt tgcttcagaa
     7201 atggttaact tatctaaaat gaatatatta gttcaagcat cacaatcaat gcttgctcaa
     7261 gctaatcaac aaccacaagg agttttacaa ttattaggat agaataaatt tagtatattg
     7321 aataaaaaag aaaggataag gctttgcttt attcttttct ttttttagaa atatagtttt
     7381 gaaaggaaaa tatatatgtt acttagtata gttatgatgg tcaaaaacga agaaaatata
     7441 cttgaaaaaa ctttaaaatc gttatcagta ttgagaaatt caatagagag tgaacttata
     7501 atagttgata caggttctac agataatact ataagaatag caaaacaata tacagaaaag
     7561 gtatattttc ataattggaa tgatgatttt tctagtatga gaaatatatc tatatcctac
     7621 gctaagggcg aatggttact tatattagat gcagatgaag tattaattga ttattcaaat
     7681 attataaagt tttttgaaag tggtcttaat aaaaaattta actctgcctc agtacgacta
     7741 aaaaatcttt attcatttga taaaaaatta tatggatatt gttcagtgtt gaggtttttc
     7801 aaaaaccttg attttaaata ctgtgggaaa gttcatgaac aaccaatgta taaaaatcct
     7861 atatttaata atattgctga ttttgaacat tatggatact tgtttgaaga tgaagagata
     7921 agaataaata aagtaaaaag aaatgaaaaa ctgttgcttg aggaattaaa agaagacgag
     7981 aaatctccat atacaaatta tcaaataggg aaaaatctta ttatattagg aaaatatcaa
     8041 gaagctttag attatctaga aaagtcttat aaattatact caaaattaga aatagtacct
     8101 ggatacttga taacaaacct tgcaaaagta tatttatact taggaaaggc taaaaagtgt
     8161 gaaaaattat gcttaaaata tattaataag gacagaaata atattgatat atattatcat
     8221 attgcacaag cacaagttga tttagggaag tatgaaaata gtatagtcat ataaaagata
     8281 tatttatttg ctagataatt atgaaatgag tacgcaagca aatagtttgc tttcagatac
     8341 tgatgctgta ggatttagag acgaagctat aattacattg ataaaaattt attataagct
     8401 tgagaaatac gatttagtaa tttcagagta tgataatatt gaagatgtgg aaaaaaagaa
     8461 aaatgtgtac tttagcttat ttatgtcctt atataaatta aatagatttg aagacataaa
     8521 aaactattat agagagatta caaattcgaa ggttgagaga gatagttttt ataaaagtat
     8581 ggaagacttt ataaataata taaaagatga tgagaaagag ctcatatata aaagcctttg
     8641 tgatattgaa ggtaattatg gtcttttgaa taaaataaga ataaacaaac atgtatcatt
     8701 agatacatgt agaaaaatac taaatgaaga aaaggatata gtttatgcaa gtgttataaa
     8761 aatagctttt gaggaaaaag ttgacttatt agacattttt tacaatatgg aatatgtttg
     8821 gatagagaaa tacttaaggt atattttagc tttagaaaga agttttaatg tatcaatata
     8881 taaatatata attagttgtc ctaatacatc agaaatagaa aagataagag tgtacaaagt
     8941 tatatctaaa attcttttag aaagtgctaa tttgagtgat aaaaaataca aagaactttt
     9001 ttatatttat ataatgtact cttataaata cataaaatac atatatagca atttgagtga
     9061 ttgtgaatta ataaagtttg tttgtaatga catagataaa ttcactttag aatttaagca
     9121 tgtacttgat ggtaaaagga agataaataa tcctaatgaa aaattagaat atctacataa
     9181 tatgagaata ttattaaatg aatacccttt ttacaataga ataataaaaa tcttcataag
     9241 tgaattagaa gaaagtataa cagaaagtga agaatttaaa ttattaaaag ataactttat
     9301 tattcgtata gaaaatatga ttgagaatgg aaaaataaat gatgcaaagt tattgattga
     9361 agactactca aaaacatttg cagatgatgt aaagatttta aatataaagg caattttgtt
     9421 tatgtgtgaa gataaatttg aagaagctga ttttgcatta aaaaaggcat tatcactaga
     9481 cttagaaaat gaggatacta tatataatat agagtactta aaaaatttaa aataaattta
     9541 aaactaggtt atttagaaaa taataatatc aataacagtt aatgaaaaat ataatagaag
     9601 attgaatata tacatataaa aatactgatg taaaatttac tggtacagca atatatatcg
     9661 atattggcat caaataaaat agaatatact caatgatttt agtatataga agatttaatt
     9721 caagggaaac taattttatc atatttactt ataataataa aatagacaag catgttcaaa
     9781 aaataaacaa caaaaaacaa tataaatgaa aattatacat attttctctt aat
//
LOCUS       DAEMHW010000139         9617 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEMHW010000139 DAEMHW010000000
VERSION     DAEMHW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9617
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.139"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            116..1480
                     /locus_tag="KPK67_002781"
     CDS             116..1480
                     /locus_tag="KPK67_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2459722.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            1502..1993
                     /locus_tag="KPK67_002782"
     CDS             1502..1993
                     /locus_tag="KPK67_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG2459723.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            2266..2937
                     /locus_tag="KPK67_002783"
     CDS             2266..2937
                     /locus_tag="KPK67_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBG2459724.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            3022..3888
                     /locus_tag="KPK67_002784"
     CDS             3022..3888
                     /locus_tag="KPK67_002784"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBG2459725.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            3921..5423
                     /locus_tag="KPK67_002785"
     CDS             3921..5423
                     /locus_tag="KPK67_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG2459726.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            5553..6005
                     /locus_tag="KPK67_002786"
     CDS             5553..6005
                     /locus_tag="KPK67_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBG2459727.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            6165..6968
                     /locus_tag="KPK67_002787"
     CDS             6165..6968
                     /locus_tag="KPK67_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459728.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            7026..7913
                     /locus_tag="KPK67_002788"
     CDS             7026..7913
                     /locus_tag="KPK67_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG2459729.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKDMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            7932..8780
                     /locus_tag="KPK67_002789"
     CDS             7932..8780
                     /locus_tag="KPK67_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2459730.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            8843..9532
                     /locus_tag="KPK67_002790"
     CDS             8843..9532
                     /locus_tag="KPK67_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2459731.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
BASE COUNT         3873 a          904 c         1689 g         3151 t
ORIGIN      
        1 aaacaacagc aataaacaaa taaaatagat aaatatatcc aattaaggta taattaagtt
       61 ttatatgata tagttatatt tgataattta caatagtaaa acttggaggg aaaaaatggg
      121 tgaaaagaaa ataaataata tgaattattc acataataat tattccaaaa agacaggtga
      181 accaactaga aaaataaaaa atagccatat cactcttaaa agtaaagaag aaaaaatttg
      241 ttctagttgc aataaagaaa ataatactaa ggataattac tgtaaatttt gtggaaatga
      301 actttatgaa atagcatcat taagaccact tgaaacaaaa ctagacttga aatctaaaat
      361 aaaagagctt tcatatcatg caaataaaag aggtgtattt ttaacaacat ttacaaccat
      421 atttatattg tttattattg cattgatatt taaagctatt ataactattc aatttaatga
      481 tattagttat ttaataaatc cagcacatat aatcctagct ctcaacttgg gtcaaattag
      541 tgtatctatg tctaccatga tgggttctgg gtttataaat gccaatatag gtttgttgat
      601 attacttata atccctattt tggtattatt aatatcaaac ctaattttta tgagaaaaag
      661 atgtagagat tcaaaaactg tattagcaaa ttctctagga gttggaattt tttatggatt
      721 agttttagct attttatcta tttttactaa tgtaaaaact agttcacata gtatgttaga
      781 atatggatat gcattagaat atagttatga attttttagt gttcttttaa atggatttgt
      841 attagggttt atatgtacat acataactac ttataaaaaa agttatgaaa aggaaaatat
      901 gtatttatct cttttttcaa attctataag aacattcata ttaggttatg ttttagtatt
      961 agtaatacta ttggtactaa ctatatctga tagtagttac ttaaatgaac tagatatgag
     1021 tagttattca aatggattaa atttatttac tatactccca caaatagcta gttatatgtg
     1081 ggcttttgca aatggaattt cagtaaccat aataaactca acagtatcaa tgtttacttt
     1141 aagttcttca tctttatttg gagatactaa attaatgttt tatgcaatgg gagcattgtc
     1201 tatgttaata ttattattaa atggatacaa gctaagattt aagtataata cagatagtat
     1261 aagacctata atagtattta gtatatatta tgcatttttg atgggaatat tggctttatt
     1321 tagtacattt atactagata gtaatattaa tttctttaat acaactaatt atggaacaac
     1381 tttaataatg caatttaaag tcttacaagc tattgtaatt tcatttgtat atagttttgt
     1441 gatatcttta ataggatata agctaaattc agctgattaa atatgattgg agggaatttc
     1501 tgtggaagga aatactaata ataaagtaga tgataaattt tctaaggtta agaaaaagtt
     1561 tggtagtgga aaatttaaag ttgaattata taaagaaaaa tcaaaattaa atagagagat
     1621 tgatgatatt caagaaaaga aagcaaagat attcttggaa atgggaatac tgacatatca
     1681 aaaaattaga gaagattcta ttaatgataa tagttttgat aaattctgtg atgagttact
     1741 agaacttgat aagttaattt atgaaaaaaa tatggaaatt aatgaaatgg aaatggcaga
     1801 aagtaatgta acttgtgagt gtggttatgt tggaaatgta aatgataaat attgtgctga
     1861 atgtggtaaa aaatttgaat tggaaaatga atgtgatgat tttataattt gtggttattg
     1921 tgaaagtgag atagatacag aggctgaatt ttgtccatgc tgtggaagaa aaattataat
     1981 ggatttagaa taatattggg aaacctatgt atagtataca tgggtttttt tattgaaatt
     2041 atttaaatgg acttataaca gtagttatat aaaatgaaaa taaacaccaa aaaaatgaaa
     2101 taaaatttct tttaaatgta aaaaataagc taatatataa aataaatcag taaaaatata
     2161 gatgcgagaa aaaaatatga aaaaatattg caaagaaaaa gttttcatgt tattattaat
     2221 acataaatca agaaaataaa tttcagaaac gaggtaattt aaaaaatgct agataaggta
     2281 aagggaagat taatagtatc atgccaagct ttagaaaatg aacctcttca tagtcctttt
     2341 ataatgggga gaatggctaa ggctgctatg gaaggtggag ctgttggaat tagagctcaa
     2401 ggagttgaag atattattga aataaaaaaa gttactggac ttcctgttat aggtattata
     2461 aagagaaact atgaagattc agatatatat ataacgccaa ctaaaaaaga agttgatgaa
     2521 cttttaacaa cagggtgtga aatgatagca ttagatgcta ccaatagagt tagaccaaat
     2581 aatgaagact taaaagaact tataaagtat ataaaagaaa acggtgttct agtaatggca
     2641 gatatttcaa attatgatga ggctatcaaa gcacaagagt acggtgtaga ttgcgtttca
     2701 actacactat ctggatatac tccatatact aaaacattag aaggtccaga ttttgtatta
     2761 atggaaagat tagttaaaga tttagaaata cctgtaatag ctgaaggaaa ggtaaatact
     2821 cctcaagatt taaaaaaggt ctttgagttg ggtgttcatt catcagtagt aggttcagca
     2881 ataacaagac cacaattaat aacagaaaaa tttgttaaag caattgaaat taattaaaaa
     2941 ttaatttata aattaaaaaa ttattatatt taactatact tgaaaattaa aattggaggt
     3001 aaagatatga gaacaacaga tatgaaggga atttattcag cattattagt ttcatttgat
     3061 aaggaaggga atataaatga aaaagggtta agacaaataa ttagacacaa tatagatgtt
     3121 tgtaaagtag atggtcttta tgtaggagga agtacagggg aaaacttcat gctttcaact
     3181 gatgaaaaga aaagaatatt tgaaatagct aaagatgaag tgaaagaaga aataaaatta
     3241 atagctcaag ttggttcagt taatttaaaa gaagctgtag aacttgctaa atttacaact
     3301 gatttaggat atgatgcaat aagtgctgtt acaccttttt actataaatt tgattttgaa
     3361 gaaataaaac actattataa tacaataata aattctgtag ataatagatt aataatatac
     3421 tctataccat ttttaactgg tgttgatatg agtttagacc agtttggaga attatttgaa
     3481 aatgaaaaaa taataggtgt taagtttaca gcagcagatt tctacttatt agagagaatg
     3541 agaaaaacat tcccaaataa attaatattt gcaggatttg atgaaatgat gttaccagct
     3601 acagttttag gtgtagatgg ggcaattggt tctacattca atgtaaacgg tgtcagagct
     3661 agacaaatat ttgaattaac taagaatgaa aaaatatcag aagcacttga agtacaacat
     3721 gtgactaatg atttaataac agatatatta ggaaatggct tatatcaaac aattaaatta
     3781 ctacttgaag aacaaggtgt tgaggctgga tactgtagac aacctatgaa agaagcaact
     3841 gatgaaatga aatcaagagc aaaagaaata tacagaaagt atttttaata aaatactttt
     3901 agtatgggag gattagtcaa atggtaggat ttacttggat agattttgca atactagtgg
     3961 tttacttatt ggcagtttta tttgcaggat tgttattttc aaaaaaggaa atgaagggaa
     4021 aagagttctt caaaggtgat ggaactattc cgtggtgggt tacatcagta tcaatatttg
     4081 ccacattatt aagtccaata tcatttttat cattagcagg aaattcttat ggtggtactt
     4141 ggattttgtg gtttgcacaa ttaggtatgt ttatagcaat acctttgaca ataaaatttt
     4201 tcttacctct atatagtagg ttagaaatag atacagcata tgaatactta gaaatgagat
     4261 acgaaagcaa aggtcttaga gtacttggag ctttgatgtt tataatatat caaataggac
     4321 gtatgtctat cataatgtat cttccatcaa tagttctggc tggtcttaca ggaatacctg
     4381 taaatgtact gataatagtt atgggtgtaa tagcaataat ttactcatat acaggtggat
     4441 taaaggctgt cttgtggacc gattttatac aaggaatggt attaatagta ggtgttacag
     4501 ggacattgtt ctatttaata gcaagtataa atggtggttt tggaacagta atggatactc
     4561 ttacaactgg acacaagttc ttagcaagta atgaagttat gtttgataaa aatatactat
     4621 ctacaagtgc atttataata tttgttggag ctggtcttaa tactttttca tcatatgtat
     4681 cgagtcaaga tatagttcaa aggtttacta caacaactga tataaagcaa cttaataaaa
     4741 tgacatatgg aaatggtgtt ttatcaatgg gacttgcaac agtattttat ctaattggaa
     4801 cttgtttatt tatatactat actcaaaacc cagatttagc tcaaacagtt caacaagacc
     4861 aagtatttgc atcatatata gcttatgaat taccagtggg tataacagga atactacttg
     4921 cagcgatata tgcagcttct caatcaactt tatcgacagg gcttaattca gttgctacaa
     4981 gttggacttt agatatacaa actatcataa caaaagatat aagtatggaa aagcaaacta
     5041 agatagcaca atatatatca ttaggtgtag gtatactttc tatagctgtt gcaatagtat
     5101 tagcaaatgg agaaataaag tcagcatatg aatggtttaa tagttttatg gggctagtac
     5161 taggtgtttt agcaggaata tttgtacttg gtgcattttg taaaaaagcg actaaaatgg
     5221 gagcatatgt tggattcata gtatcagcaa ttttagttgt ttatttaaaa taccgtatgc
     5281 cagaagtaac ttcttgggca tattctctaa taacaatcac gacttctgta gtggtaggta
     5341 caatagttag tgcaatagaa gctaaagtta aagcaaaagt atctttacca aaagctgaga
     5401 ctactgttta ttatgaaaaa taatatttaa atgataaatt gtcttataga gtgattagat
     5461 aaataattta gttactctat aagattgtat aatatacttt gtatataaaa gtataaaaat
     5521 agactttata attatatagg gagatagtgc ttatgatatt tggaaatata aatcatagta
     5581 agacatattc aacattgcat gaagatttac ttagatgttt tgaatatgct aaagataatt
     5641 cattaataga ttatgaaaaa ggtacttata caatagatgg tgatgatatt tttgtgaata
     5701 ttgtagagta taaaacttgt gaaaaagaag atagattctg ggaagctcat aagaagtata
     5761 tagatgttca tttgatgctt gatggatgtg aacgaataga tataaatttt atagaaaatt
     5821 tagaacaaaa aatatatgaa gaaaagggcg atttcttgcc tcttgatggt aaaaataatg
     5881 gatacatgga acttagaaaa ggtgattttt tagtttgtta tccggaagat gctcatatga
     5941 caggaataaa agttttagaa aaagaaaata tcaaaaaagc tatttttaag gttttggtta
     6001 gataatgata atttatataa ccactagggc tgaaaatagt gatttaactt tcagcgatta
     6061 gtgagacttc gtaaatttta gttagattac tattttcagc cttagttttt tatgagaaaa
     6121 ttgaaaataa ttaaaagctt aaaattttgt aaaggagtta acttatggaa aatatagatg
     6181 tattcttatt aataggacaa agcaatgcta gaggattggg aaatccaaaa gagagcatca
     6241 ttccaaatga aaattgtttt gaatatttga gtactgatga aattataaat atgagatgtg
     6301 agctagagac ttcagaaggt gatggtacta tagcacctgc attttcaaat gaatggaata
     6361 agcttactgg aaataaggtt tgttttattc acaatgcaaa agatggttca agaataaaaa
     6421 actggaatca tgataataac tggtttctaa atgatactat agaaaaattt aatacaggct
     6481 gtactacctt aagtaaacat tataatatag aaaataaata cgttatttgg attcaaggtg
     6541 aaagtgatgc aaaatatggt agtgatgtat tgtactataa agaaagtcta aaaaaaattg
     6601 cgtataggct taaagaagaa tgtatgattg ataaaatgtt tgtaagttta actggttatt
     6661 ggcttggaga agatgaatat tttattagaa caagacgaat tgcagcagca caggagtcgg
     6721 cttgtagtga atgtgatata ttatgtgtag gaagcaaaat tgctatgcag tttcatgaca
     6781 gaggattaac tattgatgat gttcattaca ctcaagaagg tcttaatatg ttaggagagg
     6841 acttatgtaa aaatatttat aagtatcata taaccaaaga aaaaagtgta ttaagtgata
     6901 ctatagactt atcagaagca aggaaatata tctgtgaatt ggaaaagata agtaaaagat
     6961 ttgtgtaata taaaatttta aatagagttg ttaatttata aaaataattt gtatggagat
     7021 atgttatgaa aaattacgtt tgtatagatg ttggaggtac ttcaataaaa tatggtttta
     7081 ttagagaaga tggatttata attgataaat caagtcttga tactgaagca aaggaaaaag
     7141 gtggagaagg aatattagcc aaaataaaag atatagtaaa aaaatatata gaggaaaatg
     7201 aaataagtgg tatatgtata tcaactgcag gtatggttga cccagttgaa ggtaaaatac
     7261 tatttgcatt agaagaatta attccaaact ataaggacat gcaattaaaa aaagaagtag
     7321 aaaaagagtt taatataaaa tgtgaagttg aaaatgatgt taattgtgct ggacttggtg
     7381 aaatgtggct tggagcaggt agaggtgcaa catcttctat atgtttaaca ataggtactg
     7441 gaattggtgg atgtattata ataaataata aactgataaa tgggtttagt aatagtgctg
     7501 gtgaagtagg atatatgaat gtaaatggaa gttcttttca agaattggca tctactagca
     7561 gtctaattaa aaaagtagca aaaataaaaa atttgaatga aaatgactta aatggaaaaa
     7621 taatttttga tttagcaaag aataatgatg aagattgttt aaaagaactc gataatatga
     7681 taaaatcatt agcagttgga atcgcaaata tatgttatgt aataaatcca gaagtggtaa
     7741 ttttaggtgg tgggattatg gcacaagaaa agttcctaaa accaaaaata gacaaagcat
     7801 taaaggaggt tcttatagag agagtttaca aaaatactaa tatagaattt gcaaagagac
     7861 aaaatgatgc aggaatgctt ggagcattat ataatttttt aaacaaaata taaagaatag
     7921 aagggttttt aatgagtatt ttagaacaat tggaatcacc aacttttaaa gtcacaaaat
     7981 cagataaatt attaatagct tatataaaag aaaatataga tgatgtattt tataaaccaa
     8041 tatcacaaat agctaaagag agcaatattg gtgaagcaac tataactagg tttgtaaaaa
     8101 aaatgaattt taatggatta caggatttta aagttacttt agcacaagaa atatcaacta
     8161 tcaataaaaa aaatattata aataaaaata tacaaaatga tgagccagct ttggatacag
     8221 caaaaaaact tttaagttca aatgtaacca ctttagagaa cacagtagag ataatcaata
     8281 gtaaggatgt acatgactgc gcaaggctta taataaatgc aaagaaagtt tacttcatag
     8341 gtattggata ttctggtata atagcacagg attcaaacta taaatttatg agaataggtc
     8401 ttaattgtgt aagttttgac agtagtcata caatgataat gatgtcttcc ataatggaag
     8461 aaggagattt aataattgca atttcacatt ctggtgaaac agaagagatt ataaaaactg
     8521 taaagcttgc aagagccaat aatgctaaaa ttatatcaat tactgaaaat aaaaattctg
     8581 aattaaagga tatttcagat gtacacttat cttatgtatc gggagaaaca gtactggaaa
     8641 ccggttcaat atcttctaaa ttagcacaat ttttcattat tgatttagta tatacacagg
     8701 tagtaaaaga actttctaat gaagctattg aaaggaaaat taaaactaca aatgctataa
     8761 aattgtttaa aaatgaataa aataagaaat ttgtaatgtt ttatagagct ctcttaaaaa
     8821 tttaggaatg gagagatatt acatgtctaa attaaaagat tataatattg ataaagtaat
     8881 aatttttaat aatttaagta ctaaaacgaa gaatcaactt aaagaaaaag ttaaactaaa
     8941 aagattacaa aaaaaagaaa ttttatttta tgaaaaagat agcttagaca gtgtttatat
     9001 acttttagaa ggaaaggtat ctatttttaa gataagtgaa aatggagaga gaaaagtaat
     9061 tttcatatta aatagtgggg aagtcataaa tgatattaca cttgacgctt ctaaaaactc
     9121 tactgttggc tgtgaagcat ttgaaaattc aattgtagct tattatagta ttgaagaatt
     9181 tttagaaatt atgcaaaatg attttcaact tacaaaggat attatttctt atatggaaag
     9241 aagaataaga cgtttatata gacagttgaa aaattctata tccataaaag tagataaaaa
     9301 acttgcagca aaattatata ggttaagtag agaatttgga gtttgctgtg gagaatggac
     9361 tttactaaat gtaaatatta cagttacata tttagctgat atgcttggct gtaaaaggga
     9421 gactgtttcc agaatgataa aggtattgca aaaagaagaa cttattaaaa ttgacaataa
     9481 aggtttttat gttaaagaaa ctgaattatc aaggtatttt aaaaataatt aattatgatt
     9541 aagtaagagt attattagaa ttttaattaa ataaaaatat tattaagaga atctatttat
     9601 agattctttt ttattgt
//
LOCUS       DAEMHW010000140         9427 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEMHW010000140 DAEMHW010000000
VERSION     DAEMHW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.140"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            80..814
                     /locus_tag="KPK67_002791"
     CDS             80..814
                     /locus_tag="KPK67_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459732.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            1025..2206
                     /locus_tag="KPK67_002792"
     CDS             1025..2206
                     /locus_tag="KPK67_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBG2459733.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            2190..2762
                     /gene="mobB"
                     /locus_tag="KPK67_002793"
     CDS             2190..2762
                     /gene="mobB"
                     /locus_tag="KPK67_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBG2459734.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKKILKE"
     gene            2881..3156
                     /locus_tag="KPK67_002794"
     CDS             2881..3156
                     /locus_tag="KPK67_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2459735.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            3412..5112
                     /locus_tag="KPK67_002795"
     CDS             3412..5112
                     /locus_tag="KPK67_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459736.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            5475..6662
                     /locus_tag="KPK67_002796"
     CDS             5475..6662
                     /locus_tag="KPK67_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459737.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            6722..7885
                     /locus_tag="KPK67_002797"
     CDS             6722..7885
                     /locus_tag="KPK67_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2459738.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            8036..8839
                     /gene="zupT"
                     /locus_tag="KPK67_002798"
     CDS             8036..8839
                     /gene="zupT"
                     /locus_tag="KPK67_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBG2459739.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            8935..>9427
                     /locus_tag="KPK67_002799"
     CDS             8935..>9427
                     /locus_tag="KPK67_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBG2459740.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSNSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIIL"
BASE COUNT         3671 a          943 c         1742 g         3071 t
ORIGIN      
        1 tataattatt gaaaaaagag ataactgtaa attgagatat agataaatac tataaaattt
       61 aagaaaggag gattgattta tgaagttaaa aaggtcatta gtttgtgtaa ctattttagg
      121 tattatactt gttggatgtc ataaagaaaa tactaaagag aaaaatcaag tagcaagtaa
      181 agcaacacag caaaaaacta tgacaaaagt tcaaaatgat gttaatgaaa ttatgaataa
      241 agattataag tacattataa agaatatggg tataccttat aatacatttt attatataaa
      301 acctaaagtt ttaaaagaat caaatacaat gcaagatata aatacatcaa gttatatgac
      361 attagtttat ccaaaatata ctggaaatga tgaattagat ggaagtgctt tatatgttga
      421 tattaacgaa aataaagttg tcaatgttga aaccaattct ttctcatcac aaggtatatc
      481 agtcattgat gctgaatcta gtatagtgat tgaaaaaagt gaccatgaaa aatcggctgt
      541 atcactagaa aattttagac atattgacct gggagagtat gttggtgttg aagattccag
      601 aataaatgaa attgtaggag atgcgaatta tgatttaaca gcatataatc atgaaggcag
      661 taaagtagtt aagagttata gattgaaaga agataacaaa atattaaaaa aagaggtact
      721 tacaataagt attgtggata acaaaataaa atctataaaa actattgaaa gtgataaaat
      781 tgtaaaaata ataaaaggaa ctttattaga ataaaatatt ctaaagcata tgatgctgtt
      841 tcaagataga aggaaatttc tagctgggaa cagttttttt attagattta taaaatttga
      901 actgcactta tagataacta ttagttataa ttaaatataa gataaaagta tttccaactt
      961 ttataatcta taatatatta catagaaata tcttaaataa aaaatatatt ggggagtatt
     1021 taaaatgata gaattagaag atgccataaa aattattgaa aaaaatgttt taccaataaa
     1081 cagggtcaag atagtaaatc ttatagatgc agtaaataag gtagttgcag aagatatata
     1141 ttcaacaatt gatagtcctc catttgatag gtcaccctat gatggttatg catataatgc
     1201 aaattctgaa aatagaaatt taaaggttat agggactttg tatgcaggag atgtatttaa
     1261 tagggtatta aataaaaatg aagcagtgaa gataatgact ggtggaaaga taccacaggg
     1321 tgcaaattgt gtaataaaaa aagaagatgt aacagttata ggtgatatga taactttaaa
     1381 tataaaatta aaggaatacg ataattacat atttaagggg gaggatataa aaaaggggca
     1441 attgattatt aataaaaatc agaatatagg atatgatggt attggagtat tagcaagtct
     1501 tggaatatcc aaagtaactg tttatgatga tttaaaagtt ggaattttga atacaggtac
     1561 agaactacaa gatataaatg agactttaga gaatggtaaa atatacgata gtaatagata
     1621 taccttatat tcaagattat taaaacttgg aatacaagca gatacaatat gcaattgttc
     1681 tgatgatata ttggagttag aaaaaaaggt aaagaatatg ttacagagtg tagatttttt
     1741 aattaccaca ggtggtgttt ctgttggaga taaagattta attccagatg tattcaaaaa
     1801 aataggggca acagaattat tttggaagat aaacattaaa cctggtggag cttgttatgt
     1861 aggtacattg ggagaaaagc tattatttgg tctttctggt aatccacatg cagcaatagt
     1921 ggtatttgat aatgtgatag ttcctgttat tgagtattta acatataaga agggaacaca
     1981 tcatataaaa gctatattta aaggagaatt taataaactt tcaaataaag atagatttgt
     2041 aagtggaaaa ctatatacaa gtgaaggtaa attgtatgta gagctaaatg ataagaaaga
     2101 tgcaaaagct agactatctg caactctaag aagtaattgt atgattaaga ttaaaaagaa
     2161 tgagacaata aaagaaaatc aattagtgga tgttgttatt agatagtttt atgaaaggac
     2221 acaattttat gtataatata ttgtcagttg tctcatattc aggagtaggt aaaactacgt
     2281 tgattgaagg cataataaaa gaattaaata aaaaagggta tcgtgttgga gttattaaac
     2341 atacctgtca tgattttgat atggatgagg aaggaaaaga tacttataaa catagaaaat
     2401 ctggggcgag aaaagtctgt ataatatcag ataaacgagt agcatatatt gaagaactaa
     2461 aagagaagaa tccattatat aaaatgattc aattatatga agatatggac ttgattatca
     2521 tagaaggata taaaaattat agatttaaaa gattggaagt aactagaaaa ggtaagtatg
     2581 aggatatttt atctaataag tctgatttga taggtattgt cagtgatata gaatataaat
     2641 tgaatattga acagtttaat ttaaatgatt ataaaaatat atcaaagtac atagagtctt
     2701 gtataaaaaa tgaaatttta agtataagta atgttgaaat aaaaaaaata ttaaaagaat
     2761 aattttaaga tgttcttctt atataaaatg atataattat ttatataaat aaagatataa
     2821 gaaatttatc aattttattt tgagttataa aatattaaag aaattgtagg aggacaatat
     2881 atgaaaaaaa tattagtagc ctgtggagca ggaatcgcaa catcaacaat agtttgtgac
     2941 agagtagaaa gattggttaa agaaaataat atacaagcag aagttgagca atgtaaaatt
     3001 gctgaatgtt caacaaaaca agaaggtgct gatttaatcg tatctactac tatactacca
     3061 acaacttatg atatacctac aataaaggca acaggatata taacaggtat aaatacaaca
     3121 gcattagata aaaagatatt aaatgcattg aagtagtttt gtatttataa aaaaaattta
     3181 gtattaaata tagagttgta tctttataaa attttaaata tagagatata gctcttttta
     3241 tgttgtttca agtatacaac tatatgatat aaatcttcaa ttttatgaaa aagattgtta
     3301 ttactttgta agtttataaa agatttaaag ttattgttat gttatactaa tttatgggta
     3361 ttttttagaa gtactaatag aagtactaaa taaaggaggg atttaatact tatgataaaa
     3421 caatttgtca aatattataa gccatataag aaaattttta cactagacct tatagcagct
     3481 tttctatttt ccttatgtga tttagtttat cctatgataa ctagaaatat aatggatgac
     3541 gttgttccaa ataaaaactt aagaatgtta gtagtttttg cagtagcact tatacttata
     3601 tttatagcaa aagcagggct taactatttt atgcagtatt ggggacatgt tattggtgta
     3661 gatatgcaag ctgatatgag aaatgaggta tttacacact tacaaagact tccaaatacg
     3721 tattttgata ataacaaatc tggagtaact atgtctagaa tagtaaatga tttaatggat
     3781 ataacagaac ttgcacatca tgggcctgaa gatttattca tttctatagt aatgttggtt
     3841 ggttcattct ttatacttat agatataaat attcctctta cgcttataat atttgctata
     3901 ttaccgttta taatttggtt tgcaatagct aaaaaagata agatgaatat tgcttttatg
     3961 aagagtagag ttactatagg tgatgtaaat gctactttag aaaatagtat agcaggtatg
     4021 aaggtaacta agtctttttg tactgagaaa gaagagttaa ataagtttgt aagaagcaat
     4081 aaattattta gaagagcaag acaagattct tataaggtaa tggcagaata ttactctggt
     4141 atgaatttat acatggatat cttagagtgg gtagttgtaa tagctggtgg ttattttact
     4201 tatataggaa agattacttt gggtgatttt gctgcttata tactatatgt aaaaatgttt
     4261 atacaaccta tgaaaaaatt aataaacttt acagagcaat atcaaaatgg tatgacaggt
     4321 tttaaaagat tcatagagat aatggaacaa gaccatcaaa aagaagctaa aaatcctatt
     4381 gaacttgaaa atgtaaaagg tgacattgag atagaaaata tatctttcac atatgaagat
     4441 aagactcaag ttttggataa tttaagttta tctataaaag ctggtaagac tatagcatta
     4501 gttggacctt ctggaggagg aaagactaca ttatgtaatc tattgccaag attttatgaa
     4561 tttgataagg gtgatataaa aattgatgga aagagtataa aagatgttag tttaaaatca
     4621 ttgagaaaaa atataggtat agttcaacaa gatgtatttt tatttacagg aactataaga
     4681 gataatattc tttgtgggaa ccctaatgct acagatgaag aaatgatagc tgctgctaaa
     4741 aaagctagaa tacatgactt tgtagagact ttgccagatg gatatgatac ttatatagga
     4801 gaaagaggcg taaaattatc aggtgggcaa aaacaaagaa tttcaatttc aagaatcttt
     4861 ttaaagaatc ctccaataat aatattggat gaggctacat ctgctttaga caatgtaaca
     4921 gaaagagaaa ttcaagagtc tttagaggaa ctaagtaaag atagaacaaa tttagttgtt
     4981 gcacatagat tgactactat aaaaaatgct gatgaaatca tagtattgac ggataaagga
     5041 attgaagaaa gaggtactca tgaagagcta gtaaacaaaa atggagtata tagtaggtta
     5101 cacaataact agtatacaaa ttttacacca tagctaacat acacattaaa cttacaaaca
     5161 taggatatat taagattcat aaagacaatt cattttatat ttatgagatg ttttatgaat
     5221 aaaatgtatc ctatgtttta ttttttgcaa attacattta attctctttg taagaataga
     5281 gttaaatagc caatggtcgg gataacgttt ttacaaaaag aataatttag aaaactaact
     5341 ttaatttatg gaatttatcg aattttatga aaaaaatcga attttgactt gaaatgtttt
     5401 tcaaaagtag taaaataatg gataactata catacatttt ataaaaatat atttattatg
     5461 aggggggatt ttgtatggag gcaataacag ctttttgtct agttttagta tcactgacga
     5521 taggagatat agtttcagca aaaactaaag cttttgttcc gtcggttttt gtatcagcaa
     5581 ttatatttat agtaggattt tggacttttt tcccagaaaa tattgtggac ttagccgctt
     5641 taggaacacc attagctcaa ttaggtatgc ttttgttaat aactcatatg ggaactatga
     5701 tgagtattaa ggaattagca ggtcaatgga aaacaatagt aatagccttg gcaggaatag
     5761 taggaatatg tgtaggtgct ttggcattag ggacagtagt atttggatgg gatacagcag
     5821 taatagctac acctcctctt actggagggc tagttgcatc tataatgatg gcagatgctg
     5881 ctacagcaaa aggtcttgca agtttatcag tacttgcaat tttgatgtat gttgcacaag
     5941 gatttgcggg ttatcctatt acagcattaa tgcttaaaaa agaaggaaaa agacttttat
     6001 cagactttag aagtggaaaa gtaactgtaa atacagatga agaaaaagtt aaggatttac
     6061 ctgaacaaaa aagcagattt aagataatac cagacttacc agaaaaatat gatacgacat
     6121 atatgataat acttagatta ggatttgtag catggttagc agtacaattt acaaatctta
     6181 caaatgaagt tgtatcaaga tatgttatat gtttaatatt tggtgtaata gcatcagaac
     6241 ttggaatagt tgatagaaaa ccacttaata aatctggttc atttggatgg ttaatgacat
     6301 cattgatggc ttttatattt gcagggttag caaaagctac accagcaatg cttgttgaaa
     6361 tggcagttcc tcttgtagga atcataatat ttggtgtaat aggtatggga atattctcta
     6421 ttatagtagg taagctttta ggtgaaagta aagaaatggc tttttcagtt tcgcttacat
     6481 cactttatgg ttttccacca aactatatat taactgaaga ggcagtaaag gctttggctg
     6541 aaacaccaga agaaaaagaa tttttaatgg ataagatgct tccaaagatg cttgttggag
     6601 gatttactac agttacaata gcttctgtag taatagcggg tatctttgta aatctaatat
     6661 agtatttaag tgataaaaag tattttaaat aagaagaatg tgtactactt ggaggtatgg
     6721 aatggatatt aaagaaataa caaagagtta taaagactat gtaattaagc ttagaaggga
     6781 atttcatgaa aatcctgaga aaagtatgga agaagttaga acatcaaaga gagttaaaga
     6841 agaacttgat aaaataggga taccatatgt atcagctgga ggtacaggtg taattgcaac
     6901 tataaaagga gctaatccag gaaagactgt agcacttagg ggtgatatgg atgctctaca
     6961 agttgttgaa tgcactgatg tagaatacaa atctaaaaat gaaggactta tgcatgcttg
     7021 tggtcatgat ggtcacacat caatgcttct aggggcagca aaagtgttaa atgatattaa
     7081 ggatagtatc aatggaactg taaaattatt ttttcagcca ggagaagaag ttggtaaagg
     7141 tgcaagagct atgattcaag atggtgctat ggaaggtgta gatagtgtgt ttggaataca
     7201 tctttggact gatgtagaat ctgggaccat atcggtagaa gaaggaccaa gaatggcttc
     7261 tgctgatttc tttaaaatta ctgtaaaagg tagaggtggt catggttcac ttcctcatca
     7321 aggtgttgat gcagtgcttg caagttcagc aatagttatg aatctacaat ctatggttag
     7381 tagagaagta agtcctctgg aaccattagt tgtgagtgtt ggagttttaa attctggaac
     7441 tagatttaac gtaattgcga gtgaagctgt cttagaaggt acaataaggc tatttaatcc
     7501 agagcttaga aagcaaattc ctggaatttt agaacgtata gcaaaatcta cagcagaagc
     7561 atatagagca gatgctgaat tagagtatgg gtacttaaca ccagcagtta taaatgacaa
     7621 ggaatgttca aagatagcta cagatgcagc aattaagcta tttggagaag actgtataac
     7681 actatttgaa aaagtaacag gagcagaaga tttagctgag tttatgaata tagcacctgg
     7741 tgcactagca tttgtaggag ctagaaatga gtcaaaaggt gcttgttatc ctcatcatca
     7801 tggatgcttt aatatagacg aagatgctct agaaattgga actgctttat atgtgcaata
     7861 tgcagtggat ttcttaaata aataatttta atatactata aagaaaaagc agagactaag
     7921 tttatatctc tgctttttta ttgtgtatat tttttataaa tattgactat ataaaatata
     7981 tatgatagta taaagaaaat gataatcaat atcaattaaa aagaaggtga tttatttgaa
     8041 taatgtaata tttgcatttt tgataacatt attggctggt ttatctactg gtataggaag
     8101 ttgtatagca ttttttgcta aaaaaacaaa tactaagttt ctttctataa gcttaggatt
     8161 ttctgctggt gtcatgattt atgtttctat gatagaaatt tttcctaaag cacaagatgc
     8221 tttgacaaaa tctatgggag agaaattagg aagttggtca actgtaatag ctttttttat
     8281 aggtatggct atcattgctg ttattgataa gctaattcca caagaagaaa atccacatga
     8341 aataaagaaa atggagaata tagaagaaaa gaatatcaaa aaaaataagt ctcttttaag
     8401 aacaggtata tttacagcaa tggctatagc aattcacaat tttcctgaag gattagctac
     8461 atttatatca gctcttgatg atgttacaat agctattccg atagcaattg caattgcaat
     8521 acacaatata ccagaaggta tatctgtatc tgtaccagtt tattatgcca caggagataa
     8581 aaagaaagct ttttattatt cctttttatc tggtatgtct gagcctttag gagcgattat
     8641 tggatatgta cttcttagaa attttttaaa tgatataact cttggaattg tatttgcaat
     8701 tgtaggagga attatggtat tcatatcact agatgaatta ttgccatcag ctagagagta
     8761 cggggaacat catttatcta tttatggatt gattgcagga atgggtgtta tggctattag
     8821 tttattacta tttaaatagc tttttaaata tatgattgta acctaaaatt agtatcaaaa
     8881 tgatataatt atttaaatag atttttaagc tatttaaaaa tatcattttg gagaatgagt
     8941 gctatggaaa agaaaataaa attcaaattt gactggtata gttcacctga taagatagag
     9001 aaatggttag aagatattga agctaaagga tataatttat ataagataaa taaatttgga
     9061 ggaatatttt attttcataa gggaagtcca agaaaaataa aatacactgt tgattactac
     9121 tatgatgtag agaataatta ttttgatgaa cataaatcaa atggctggaa tcttgtattt
     9181 aggagtattg gtagttttat gggtacatct gcatggtata tatggtcgaa agaatacaaa
     9241 gacatacctc ctaaattata tagtaattct gataaaaaaa acaaagtaaa gtcaatttta
     9301 tttatgaata tgtttatatt ctttatgtta gtagtaatat ttatatattt attgaatatt
     9361 gaaataagaa atttatatag tggttatgga gcatctgtaa ctgctattgt actacagatt
     9421 atattgt
//
LOCUS       DAEMHW010000141         9426 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEMHW010000141 DAEMHW010000000
VERSION     DAEMHW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.141"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            178..807
                     /locus_tag="KPK67_002800"
     CDS             178..807
                     /locus_tag="KPK67_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459741.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            808..1590
                     /locus_tag="KPK67_002801"
     CDS             808..1590
                     /locus_tag="KPK67_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2459742.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1583..2461
                     /locus_tag="KPK67_002802"
     CDS             1583..2461
                     /locus_tag="KPK67_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459743.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2494..3453
                     /locus_tag="KPK67_002803"
     CDS             2494..3453
                     /locus_tag="KPK67_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2459744.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3766..4458
                     /locus_tag="KPK67_002804"
     CDS             3766..4458
                     /locus_tag="KPK67_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2459745.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4468..5472
                     /locus_tag="KPK67_002805"
     CDS             4468..5472
                     /locus_tag="KPK67_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2459746.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHHMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            5740..6591
                     /locus_tag="KPK67_002806"
     CDS             5740..6591
                     /locus_tag="KPK67_002806"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG2459747.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     VTNIISV"
     gene            6695..7441
                     /locus_tag="KPK67_002807"
     CDS             6695..7441
                     /locus_tag="KPK67_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459748.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7441..9357
                     /locus_tag="KPK67_002808"
     CDS             7441..9357
                     /locus_tag="KPK67_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2459749.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENKLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNNNVKENIIVENRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIET
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYIVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
BASE COUNT         3671 a          884 c         1661 g         3210 t
ORIGIN      
        1 tgtatgcatt attaattaaa ataaattaca tatactattt aatatttcat ttaaaatatt
       61 aaaaaatgta gataaaaatt gttttattaa gaaaaaatta atatattgac aaaatttaag
      121 tgctcattta aactattaat tgataataat attcaatatt aaataatagg aggcgttatg
      181 aataaatatc ttctaataaa tccagtggca gaaagaatgt atggagaaaa gtttcacctt
      241 attaaagcaa atttgataaa aaaggggtac ataatagcag aatgtgaacc acagttagaa
      301 tatgtaaaaa agcagtacaa agagtataca aaaaagactg aaaatacaat gttggattgt
      361 agatgtccag aaagtataag tcttttaaaa agaaataatt tgataaatga ttttgaagta
      421 cctatgatag aaccaatatt agtaaggact tgtagggttt tatacgataa atatataaaa
      481 agtaaagatg atatgttggt agtaacctgt ccatgtacac aattaagaga ctttgcaagt
      541 gaaaagttta aggataaggg taatgctatg ttttatacat ggaaagagtt tgctgaacaa
      601 gaaggtgtaa aatcacttgg aaaaatagat gaatcaccaa ttccattagg atatttcaaa
      661 gatacttttg aaaaagttct tgaagtatca agtgaagacg aaatattaag tgaaatacaa
      721 aaaataagaa atggctttga agataagtat gacatagtag aaatgtttta ttgtaaagat
      781 ggttgtaata atggagatgg tttgtaaatg gtcaataaat ttaataataa gtttttaagg
      841 ctagtaaaga gtgttttgat attttttata attcttttat tatggaaaat tacaaattat
      901 cttggtattt ggagtgatta tatattacca tcacctgaaa aggtatatag tacattttta
      961 aatatgataa gtgatggttc aatatttatt aatgtatatg caagtatgaa aagagttttg
     1021 ataggttttg caataagtac tgctataggt gtgcctttag ggatattctt tggaatttat
     1081 agtggagtat atgaatactt taaatctcta ataaattttt taaggaatac acctcccctt
     1141 gctttaatac caatgcttat attatggttt ggtataggag aggaatcaaa aattataatt
     1201 atagttttag catccttttt cccaatcttt acaagcacat tgaaagggat taaaaattgt
     1261 gactcaaaac tgattgaagt gggtagagta tttgagtttt caaagcttca gataatattt
     1321 aaaataataa taccaaatgc cattcttgat attgctgttg ggttaaagtt agctcttgga
     1381 tatagcttta gagcaattat tggggcagaa cttgtagcag cttcatctgg tcttggatac
     1441 ttgatatctg atggaaaaga aatgtctaga acagatgttg ttattgtagg tataatcgta
     1501 attggacttt taggaataat tacagattac atattttcga ttattgtgaa gaaggtaagt
     1561 aagggaaaga tggtggaagc atatgaataa tttagtatgt gactctggat atataattag
     1621 aaatttatat aaaagttata aagttgacaa tgaagaacat gtagttctta gtgatatctc
     1681 tttaaacata gatagaaacc atataacagt aattttaggt gaaagtggtt gtggaaagac
     1741 taccttgctt agagcaattg catcacttga aggtataaat agtggtgaaa ttaggtttat
     1801 taatgataat aaagagtata gacctaatgt tggttttgtt tttcaggaga gcagattaat
     1861 gccttggtta aacgtaagtg aaaatatagc ttttcataat caaagaaaaa atactatttt
     1921 aaacaaggtg cttagaaaat ttaaacacaa taaaagaatt agcagtaaaa ttataaatga
     1981 taattttaga agcttagaaa caaatgataa tttgaacaaa tcaaataaat ataataggaa
     2041 aagtaatatt gatgtagaaa aatatcttaa gatgatgaac ttgcagaaat ttaaaaactc
     2101 ttatccaaat gaattgtcag gaggtatggc acagagagta agcatagcta gagctctttc
     2161 tttcaatcca gatatgttgc ttatggacga gcctttttcg gcattagatt attttacaag
     2221 gattgatatg caaaatgaag ttataagaat acataagtca actaataaag gcgtgatatt
     2281 tgtaactcat gatattgatg aagcgttaaa gataggcaag aaaatagttg ttttcacaga
     2341 cgaaagaaaa ataaaagaat ttaatataga agatggttat aatagagatt taacttcaga
     2401 ttactatatt aaattaaaaa aagatatttt aatgacaatg aaatcaaata aaggcattta
     2461 aagaaattaa taatatttta aggagtaaag taaatgaaat taaaaaagct attgataggt
     2521 ataacgatat taacacttgc aacagcatct ttagctggat gtgctaaaaa aaataatgga
     2581 gaaaaacttt cagaaataaa tttaacttat gtaaaatcac cattaaatgt tccttctata
     2641 attcaaaaac aggatgattt atttggaaag gaatttaaaa aggataatat aaaagttaac
     2701 ttccacgaaa ttactacagg tccagagcaa acacaagctt tagctgctgg agaaatagac
     2761 ttcttacatg cactaggtgg tacatcagct ttaatagctg catctaatgg agtagagtta
     2821 aagatattaa acacttatag caggtcacca aaaggattta tgattttgac taataataat
     2881 tcaataaaat cagcagcaga tttagtaggt aagaaggtgg caggcccaaa agggactata
     2941 ttacatcagg tattgatttc agcacttgat aaagaaggat taagtatgga tgatgtggaa
     3001 tttgttaata tgggtattcc agaggcatca gcagctcttt ctgatggcag cgtagatgct
     3061 gcattgattg ctggtccagc agctcttaaa gctatgaagt caggtagtaa acttgtagca
     3121 aatggtgaag gtttagtaga tggtataata gtaactgctg ttagcacaga ctttgcagag
     3181 aaacatccag aaatagttga aagatttatg aaggttgaaa aggaaacttt agaatatgta
     3241 aataataatt ttgatgaagc tatggaaaaa gtagctaaag aagttgattt aagtcttgaa
     3301 gaaactaaag aattatatgc ttggtatgat tttagtttag atataacaga taaagacata
     3361 tcttcgcttg aagatacaca agacttctta ataaaaaata agttacaaga gaaaaaagtg
     3421 aacataaaag aattaatcta taatgctaag tagaaactat gataaaataa tactaagtaa
     3481 gaaatataat aaaagtttaa gttaccttat taaggtaact ttttctattt tgaattagta
     3541 atttcaattt attttagaat gattagttga taataatgat taattaatca taaaaagaac
     3601 ttttttatag agttttatta agatgtctaa taatatggtt atacttatga agtcagtaat
     3661 tgtgaagtta gtaatatgat taaatttata tattgtaaaa agattataat atattaaaat
     3721 tggactgtta tatgatgagt aaataggtat attaggagga attatatgag ttataaagta
     3781 tatattgtgg aagatgatat atcaataagt acattactta aagattatat tgagaaatat
     3841 ggatttgaag tgattgttga agaaaacttt gatgatataa tgattacatt tgaaagtttt
     3901 aagccacact tagttctatt agatgtaaat ttacctaaat ttgatggttt ctactggtgt
     3961 agatgtatta ggcaacagtc gaatttacct ataattttta tatcagctag agatggtaat
     4021 gtagaccaaa ttagagcttt agaaagtgga gcagatgatt atataacaaa acctttttac
     4081 tatgatgtag taatggcaaa aataaagagt catataagaa gggtatatgg tgaatattca
     4141 cctaaaatag acgaaagaat agtggagcta gatggattaa aattttatcc agaaaggtta
     4201 gaactagaat taaatggtga gaatctaatt ataactaaaa aagaaggtat actgattgaa
     4261 tgtcttatca agagataccc aaaagtagta agtagagatt atcttttaga aaagatatgg
     4321 gatgatattg agtttgtaga ggaaaatact ttaaatgtaa atgttagcag gataaggaaa
     4381 agatttgagg aattaggtat agataatgct gttcagactg tccgagggtt aggatataaa
     4441 ttaaataaga attggtaagg agaaagtatg aagttatttt taaaagatta taaaggttat
     4501 atttttatat attatatagg catagttatg acaattattt actgtaatct tatgaagttt
     4561 attagttttg gagagagttt atatattctt ttatttagtt cttttatatt gttatgtttt
     4621 ttaatatata agtattataa aaatagagaa gtatataatc tgtttgaaaa aggagtacat
     4681 tctttggagg agtcattttt gtatttagga agctcattct tggggagaaa tatatcagtt
     4741 attttaaaac aacaacataa attgtatcaa tcaatgatac aagagcataa aaagatacat
     4801 agtgaacatt taactttcat caaccaatgg attcatcata tgaaaacacc aatatcagtt
     4861 ataagccttc agcttcaaga ttatgaggga gaagaagtaa ctgaaaatat aagaaaagaa
     4921 atagatagat taaataaagg gttagatatg gctatgcatt ttgcaagggc agataatttt
     4981 cagaaagact ttatagttga aaaagccaaa ttaaatgaaa ttgtatctag tattgtaaat
     5041 gaagaaaaaa ggttatttat aaaaagtgga atgattccta agattcaagt agacaagttt
     5101 acttatgttt atacagatgt aaaatggatg aaatttataa taggtcaatt aattacaaat
     5161 ggagtgaaat attctaaaga ttatagtaac tacctaacta taaaatctga agaaaataat
     5221 aaatatgtaa ttttaagtat aatagatgaa ggaattggaa ttgtttctaa agatttaaaa
     5281 agggtatttg acccattttt tacaggagaa aatggtcgta agtttggaga atctacaggt
     5341 atgggtcttt atctagtaaa aaaggtatgt gatgatttag gccataaagt aagtataaaa
     5401 tcagaagtag gaaaaggtac agaagtttca atctacttta ataaagatgt aaatagttta
     5461 ataaaggtgt aaatagtttg ataaagatgt aaattatctg ttacaatagg gtttttagtt
     5521 aattgtactt ttttattata caatttatta aatgaaatat tcatagttaa atagtcaaaa
     5581 tttaagagag tttatttgca aaccttacat agatgtaagg tttgtgtaat gtttgcagat
     5641 agttaaattt tctaatctaa attacaatta atataagaaa taacataaga gaaataacat
     5701 aaaaaaagaa attttgtaaa aaaggaagga tgtttattga tgagtttaga tgttattttt
     5761 atattgaaat cagtcataat agctatagta gaggggctta cagaatttat tccagtttca
     5821 tctacaggtc atatgatttt ggttggtaat ttaatagatt ttaaggggca atttgctgaa
     5881 atgtttgagg ttgtaataca acttggggct atattagcag tagtagtact ttattggaaa
     5941 aagataaaag atagcgttat agaatttttt aaatttatat ttactggcgg aaaagaagga
     6001 aagattggtt ttagatttgg aatgaatgta attataggtt gtataccatt tgcgattata
     6061 ggagttctat tttacgataa tataaaatca ttgtttaatt tacaatctgt aattatagga
     6121 tttatagtgg gtgggatttt attattagta gtagaaactt tgtttagaaa aaagaatcat
     6181 tccacagata atatagataa aattactcca atacaagctt taaaggttgg gacattacaa
     6241 gttttgtctg catggccagg tatgtcaagg agtgcatcta ctattatggg tggatggata
     6301 gcaggactta attctcctac agcagctgag ttttctttct ttttagcagt accagctatg
     6361 gtggcttctt caggaaaaga tttatttgaa tttgattatt caataatgac accaacattg
     6421 tggattgctt tggtagttgg gtttatagta gcttttattg tatcaataat agttatggaa
     6481 aaattcgtga attttttaaa gaaaaaacca atgagagtgt ttgctgtata cagaataatc
     6541 atgggtgtag tacttgcagt attagctgtt actaacataa ttagtgtata gaaaaaatag
     6601 atttattatc tataaaaatt aaactaagat aaattataat taacaattat atatatgtta
     6661 tttcaaaaat taaattagga aggatagtta aataatgaaa attttaactg taaataattt
     6721 aagtaaagta tatggtaaaa aaataatttt caacgcatta aatgacataa attttagtat
     6781 agaagatgga gaatttgtag gaattatggg tccatctggt agtggaaaaa ctactctttt
     6841 aaatatgatt tctactattg acaagccaac tacaggaact atggaattaa agggcaagaa
     6901 tcctttattg ttaagaggtg aggaacttgc attatttaga agaagagagt taggttttgt
     6961 atttcaggat tttaatttat tggatacatt aactatagga gaaaatatag tattgccatt
     7021 gacattagat aaggtcagtg taaaagagca agatgagaga ttgaatgaag tatctacaat
     7081 attaggtata aaagatttat taggaaaaag aacttttgaa gtctcaggtg gtcaagctca
     7141 gaggactgct attgctagag cattgattaa caatccatca atacttttag cagatgaacc
     7201 aacaggtaat ttagattcaa agtcatcaaa ggttgtaatg gagctatttc aaaagataaa
     7261 taaagaaaat aaagttacta ctatgatggt aactcatgac ccattagcag cgagctactg
     7321 tagtagaatc ttattcataa aagatgggtc tatatataat gaaatatata agggtagtag
     7381 tagagagcag ttttatcaag agataatgga tgtacttaca ttattgggag gcgataacta
     7441 atgaatataa aacagtttgc tattaacaat atttcaagaa atattaaagc gtatttagga
     7501 tacttagcaa gtattgttat atcatcttca ctgctatttt cttttattat gtttacaagt
     7561 catccagatt tagatgtatc tcaatttcct gattatttaa aagaaggtct caagatgagt
     7621 aagataattg catatctatt cttattcttc tttgtgtttt attcagttag tgtgtttctt
     7681 aaaagtagat ataaagaatt tggaatactt tatattacag gtatatcaaa acgccaagta
     7741 atgaaattaa tctttataga aaatatgata ataaatatag tatcttctgt aattggaata
     7801 ataataggct taatctttgc aaagatattt ttagtggtta tgtctacttt tttagaatta
     7861 tcaccattaa aattctatat accatttaat gcgatattgt ctactttaat ctattttatg
     7921 attttagctg tattaacatc aatatttact tcttttatag ttaaagaaaa tcaggtgttg
     7981 aagttactta aaggtacaaa aactccaaaa ccagagccta aaacatcact aatgcttgct
     8041 atattaagta tttgtttgtt tataatagcg tattataatg ctattacctc aaccgaccaa
     8101 tatacaacat atctgcgctt agttccagtt acatccctta ctatagtagc aacttacttg
     8161 ttcttttctc aatttagtgt attttttatg aagaagctaa aactaaacaa gagattttat
     8221 agaaaaaata caaatatgct ttggatttcg aacttgattt ataaggtaaa ggataatgct
     8281 agaatatttt tcttgataac tataacttct gcagtagcat ttactgctat aggtacagtt
     8341 tattcctttt ggaaagatgt agagcgtcaa attaatttaa tatatcctaa cacaatatat
     8401 tattcaacaa tgacattgca taatgaccct aaaaagcctg atagcaaaga aaaagacaag
     8461 gaaagaatga tgtttataga aaataagcta aaaaaagaaa atgtagacta tacaaggact
     8521 aatggtgaat ttaaaactgt atttcccaag aaaagtgatt ttaatgtcag aattatgaaa
     8581 gaatctaagt acttagaaat tacagaaact ataggggtta agcctataag ttttgaagat
     8641 aatgaatgta tatcattatt atcatcaagt ttaccaggaa ataataacgt caaagaaaat
     8701 attattgtag agaatagagg tttaaaggtg gcaaaacaag tggaagaatg tgttatgcca
     8761 gcatattaca aaaatatgta tgtggtaaag gataattttt atgatagtat agaatcaaaa
     8821 tttatagtgg atagatttac tgcatttgaa gtagaggatt caagtaaaac tatagagata
     8881 tgcagagaat ttgaaaataa atttaataat gagtcaggga tgcaaccata tttgtttttc
     8941 tctaaagaat taatgataga aactggtaaa ttaatgtatt ctacatttat atttttagca
     9001 atatttatag gtttaatttt ctttgttact acaagtagtt ttttatataa taaattttat
     9061 atggattgcc aagttgataa gaaaaagtat gaacagttaa ataaattggg tatgacatat
     9121 aatgagatta aaaaagcttc aacaattgag attggaatag tatttttgtt tccttatata
     9181 gtggctgtta tacattcaat atttgcactt actgcactta aaaattcttt tgatatagaa
     9241 gtagcatcat cagctgtttt ggtaatggga agcttgttct tggttcaaat agtttatttt
     9301 ttaattgtaa gaaataatta tttgaaagag ataaggttaa atttagtcaa tttttagtta
     9361 ctattattaa atgataataa aaagggttta aaattaatta atattactgg aattatagat
     9421 gtttat
//
LOCUS       DAEMHW010000142         9423 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEMHW010000142 DAEMHW010000000
VERSION     DAEMHW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9423
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.142"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            398..1603
                     /locus_tag="KPK67_002809"
     CDS             398..1603
                     /locus_tag="KPK67_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG2459750.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1674..1895)
                     /locus_tag="KPK67_002810"
     CDS             complement(1674..1895)
                     /locus_tag="KPK67_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459751.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2036..2635)
                     /gene="recR"
                     /locus_tag="KPK67_002811"
     CDS             complement(2036..2635)
                     /gene="recR"
                     /locus_tag="KPK67_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBG2459752.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2648..2998)
                     /locus_tag="KPK67_002812"
     CDS             complement(2648..2998)
                     /locus_tag="KPK67_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBG2459753.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3057..4694)
                     /gene="dnaX"
                     /locus_tag="KPK67_002813"
     CDS             complement(3057..4694)
                     /gene="dnaX"
                     /locus_tag="KPK67_002813"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBG2459754.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSINIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4771..5035)
                     /gene="ffs"
                     /locus_tag="KPK67_002814"
     ncRNA           complement(4771..5035)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KPK67_002814"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5073..5164)
                     /locus_tag="KPK67_002815"
     tRNA            complement(5073..5164)
                     /locus_tag="KPK67_002815"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5128..5130),aa:Ser,seq:gga)
     gene            complement(5165..5620)
                     /locus_tag="KPK67_002816"
     CDS             complement(5165..5620)
                     /locus_tag="KPK67_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBG2459755.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5941..7212)
                     /gene="serS"
                     /locus_tag="KPK67_002817"
     CDS             complement(5941..7212)
                     /gene="serS"
                     /locus_tag="KPK67_002817"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG2459756.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7338..7543)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7671..8552)
                     /locus_tag="KPK67_002818"
     CDS             complement(7671..8552)
                     /locus_tag="KPK67_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG2459757.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8564..9073)
                     /locus_tag="KPK67_002819"
     CDS             complement(8564..9073)
                     /locus_tag="KPK67_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBG2459758.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            complement(9200..9316)
                     /gene="rrf"
                     /locus_tag="KPK67_002820"
     rRNA            complement(9200..9316)
                     /gene="rrf"
                     /locus_tag="KPK67_002820"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3013 a         1648 c         1007 g         3755 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata tcatctttat ctattctagt caatataatt
       61 ttttactttt tttaaatttt ttatttatca tgtattcttt atatttaatt tttaaaactt
      121 aatgctaagt aaacctattt atttcataaa actaatatat attctatgct ctattttcct
      181 ttttattcta tatttaaatt ttttatttac aagaatattt ttaatataac atatttgata
      241 aaattaattt acatttcttt cacttgtaat ttttgcaatc ttgtattaga ttaatattaa
      301 aaataattta tcaataacct tattcacaaa gacagttttt atatatcaaa atataataca
      361 atataaatac tatgctacat tttaggagga ttttattatg agcaaaaaag tattaataat
      421 gtctgcttcc actggtggtg ggcataatag ggctgctcta gctattaaag aagaacttac
      481 ttccaaaaca cttgatggag agccaattga atgtgaaata atagatagtt tgaaattagt
      541 taataataca atggataaaa taatttcaag aggatacgaa aaatctgcta tatatactcc
      601 caaagcctat ggaagtgtat atagattatc tgaaacaaat cttttgtcaa aaaatgaatt
      661 taaagataat ctactaataa cattcatggc taaaaaattt aaaaagctta ttagaagcga
      721 aaaaccagac ttgattatag gaacgcatcc ttttccaatg attgctctaa gtacattgaa
      781 aaaaaacttt aatttacata ataatgaatc aaatgcttac acagaacatt tttataaaca
      841 ttatacaaat accattaatg ttcctccgct tatttctgta ttaaccgatt atactactca
      901 ttctacatgg atacaaaatg aaattgatta ctatattgtt ggtcatgaat atgtaaaaga
      961 attattggtg tttgatggag ttgaaccgag taaaattaga acatttggaa tccctgttga
     1021 aaaatctttt ctctctcaca gagataaaga tattgttctt tctgagttga atttatctcc
     1081 tgataaatta actgttttac ttatgggggg tagctttggg gctggaaata taaaagagac
     1141 acttgatgaa ttactggata ctgatagaga ctttcaaata cttgtaatta ctggtaagaa
     1201 tgaaagccta aaagaaaaaa tcgaaaaaaa acttatgtct cgttatcatg ataaaaatgt
     1261 atgtgttctt ggatacactg ataagatgaa tgatatttta gcttctatag atgtattagt
     1321 aagcaaacct ggtggactta ctacaactga ggctctttta aaagatgtac ctatgatagt
     1381 tccttattat ataccaggtc aggaagaaga gaatcttgat ttcttatcta attgtggtgc
     1441 agcacttaga actacaaaaa aatataactt gtcaatatta ctaaaagttt taatagatga
     1501 tcctagcaga ttagaaatgc taaaaagaaa tattaaatca ataagaaagt caaattcagc
     1561 ccaaaatata gctaatttaa ttttaaatat aatttctggt tgataaaata aataaaatcc
     1621 ctatagtata tatattatct attagggatt ttttattttc taattattta atattaaatt
     1681 ggctcataaa agtcttcttc ataatcatac tgataatttt tataattatt tctaaattca
     1741 gcatttttat ttcttacatt ttttttagtt tttttcttat tagaaaaact tatatcctca
     1801 aaatcatcat atttaggttt atcttttgat ttcatttttt taactctttt ttctttcatt
     1861 gcattatgtt cttctaagtt cataattggt ttcatgttgc acccctatca aaatttttaa
     1921 atgatttata caacaaatta tacaatacag aagaaattta ttcaataaat ctaaatcaaa
     1981 attaatttga atttaactca aaaaattaca aaaatcgagg tattaacctc gatttttata
     2041 tttctcttct gccttctagt gcttttgata ttgtgacctc atctgcatac tctaaatcgc
     2101 ctccaacagg aagaccatga gctatccgag tgactttgat tcccattggt tttactaacc
     2161 tagcaatata catggctgta gcttcacctt caatagtagg atttgttgcc attattattt
     2221 ctttgacttc ctgattgcca agtctttgga taagttcctt tatttttaac atatctggtc
     2281 caattccatc cattggagaa attactccat ttaatacatg atactgtcct ttaaactctc
     2341 tcgttctctc catagctgct acatctcttg gatcttccac aacacatata acacttgaat
     2401 ctctactttt atttgaacac atgctacatg ggtctgtatc tgttatatta caacaaattg
     2461 agcaatatct tatttctctt tttgcatcta taatcgcttt agataaagct tccacatcat
     2521 tagtattcat attaataata tgaaaagcca atctttgagc agtctttctt cctactccag
     2581 gaagctttga aaattcctct ataagtctcg ttatgggacc tgtataaact tgcataaata
     2641 ccacctacta gaataaccca ggtatattca tacctccagt taccttactc atttgacttg
     2701 cttgtatatc atcaatattt ctcaatgctt gatttactgc acttaaaact aaatcttgta
     2761 gcatttctat atcatctgga tcaactactt ctggcttgat agttatatct ataacttctt
     2821 ttttaccatt aacttttaca gtaactgctc ctccaccaac tgaagcttct acttctttac
     2881 tttctaactc ttgttgagct ttttgcatat tttcttgcat tttttgagcc tgctttaaca
     2941 agttgttcat atttccacca ccaggcatca ttccgcctcc aaaacctttt ttagccataa
     3001 acactattcc tcctaaactt attttcattt tattattata tcataatagt attttattat
     3061 ttactctcat tttcatcaat actatctttt atttctaata tctcttcact cactatgttc
     3121 tttaatatct cctcaccaat gtcctttttc tctttaactt caagatttat atctttaact
     3181 tctgatgtca gtactatttt tacattaaaa cttctattta aaacatctct tattgttttt
     3241 tctaaataat ttattgtatc ttgagagctc agtctagttt ttgcaaaatc aaatttatca
     3301 tcaaatataa catacaacat attagaatat acattaaaac tttttacttc acttaagagt
     3361 gcgtatattg gcattttttt atctttttta atttgtttaa gtatattctt ccaagatgac
     3421 tctataagta taacgtcctc attttttaca tcttcataaa ctacattttc ttgttttaca
     3481 tctatttctc tatcgacctc atactctttt tctttattat tcccaatttt ttcagactta
     3541 aagttcccca gttcaatcat tttttctaag ttctcaactc tttttataag agcttcttta
     3601 ctttcatcaa acattggttg agcaattttc attatggtta tttccactaa aacccttgga
     3661 tttgaagatg actttatatc atcctgagtt attgataata tatttaaaat ccttattaaa
     3721 tcatttatat ctatattttt agactgaatt ttaagttgtt ctatagtttc ttctggtaat
     3781 gaaattattt catccaattc tgatgaaacc ttacatacca ttaaatttct aaaatgatca
     3841 attaagtcat ttataagatt tcttatatct ttgccccata agacaaattc atttaatatc
     3901 tcaagagact ttttagtgtc ctgctctaca atacattgag acaattcaaa taattgttct
     3961 atatttatgc ttcccattaa ttcaacaaca tctttataat ctatcttact ttctccaaaa
     4021 gacatacatt ggtctaatat actaagtgca tctctaagag ctccttgaga atttctagct
     4081 attaaattaa gagctttatc atctatatca attccttcat cttcacaaat ctttttcatt
     4141 ctaagggtca tatctttaac tgtaactctt ttaaaatcgt atctttggca tcttgataaa
     4201 atggttgcag gtattttgtg aggctctgta gttgctaata taaaaattac atatgatgga
     4261 ggttcctcta aggtctttaa taatgcatta aaagctcctt gagacaacat atgaacttca
     4321 tctattatat atactttata tttagcattg gctggagaat atttcacact ttctctaagc
     4381 tctcttatat catctacact attatttgaa gctgcatcta tttctacaac atccattgta
     4441 ttatctttta atacagattc gcatatctca cactcattac acggttcttc atttatttta
     4501 ttcaaacagt ttactgctct tgaaaaaatc tttgcagtag atgttttacc agtacctctt
     4561 gttccacaaa acaaataagc atgtcctata ttatcactat atatttgatt ctttaaagtc
     4621 ttaataatat gatcttgacc aataacatct tcaaattttt gtggtctata tgctctatat
     4681 aatgctttat gcatcttttt acctctcttt agcaaaatta ataaaaaaat ccctcattat
     4741 ctaagggatt agtcttttat atattaaatg ctgtacacca tctatcgact taattccata
     4801 ggcgttacca aaacagttag ctcaacttag gcacccccac ggcacacaaa agtgatcact
     4861 taccgctgct tccttccgga tctgacgggg ttcatgagct ttcattgcgc aggacctaag
     4921 catcaacacc acttatttag ggcggcccta cagaaaataa gcctttagat ggaattcaat
     4981 cctgctatag cggattgcag gttacagggc accgctacct ccccatctag tacagcaaaa
     5041 ttacaaccat atttaattac tttcattgtt tctggcggag agagtgggat ttgaacccac
     5101 gatacagttt tacccatata ctcgctttcc aggcgagcgc cttcgaccac tcgaccatct
     5161 ctccttaatc tctttttctc aatgccttaa aaaattcttg caatatgatc gagcattctt
     5221 cttgcaaaac atcaaaagct acttttacat tattattatt aaaataatct aatttaaact
     5281 catgctgttt ttctatatat gaatttttta catgtcttgt ccctattata agctttttta
     5341 ttctagaatt aactattgcg ccactacaca ttatgcaagg ttccattgta acatataaat
     5401 cacaatcagt caacctccaa ccacctaatt tttctgatgc ttgttttatc gctaaaattt
     5461 ctgcatgtgc tgtagagtct tttaacgttt ctgtaaggtt atgagctcta gctattattt
     5521 gattatcttt aactataata gcacctattg gtgtttcttt tttattatat gctttatatg
     5581 cttcttttaa tgcttctttc atataaaatg aactttccat aattcctccc aaaacttata
     5641 acaaacttat cttatcataa ttcattttat aattcaactt aattttattt tgtttattta
     5701 attattacta tatatagcta tatttttttt aaattagttc taaaataatt tttagatatt
     5761 catacaagag tgttttataa aaatatttag tgttttatga tgaaaattct gctgtgtaaa
     5821 ttaacataaa aaaactatga aattaatatt tataataatt tcatagtttt tttatttatt
     5881 gtttactcat aatatattct cttcatcaaa gcatactcta catatacaat taatataaat
     5941 ttattttata acatcaacac ccatataagg tcttaattct tctggaacta ctacagaacc
     6001 atctgcctgc tgataatttt ctaatatagc agccaaacat cttcctatcg ctaatccaga
     6061 accatttaat gtatgaacat attctgcttt tgatttttta tctcttttaa atcttattcc
     6121 tgctcttctt gcttggaaat cttcaaaatt tgaacaagaa gatatttcta catatctatt
     6181 gtagcttggc atccaaactt ctaaatcata tttaaatgca gctgtaaatc ccaaatctcc
     6241 tgtacatatt tttacaactc tatatggaag acctaacatt tgtaacatag tttcagcatc
     6301 atgagttaat gattctaatt cattataaga atcttctggt ttaacaaatt taactaattc
     6361 aactttatta aattgatgtt gtctaactaa acctcttgta tctcttcctg ctgaacctgc
     6421 ttcagatcta aaacaaggag tatatgcaca gtatttaatt ggaagctctg caacatctaa
     6481 tatttcatta gcatgtatat tagtaacagg aacttcagct gttggtatta agaaatactc
     6541 taatccttct attttaaaca tatcttcttc aaatttagga agttgaccag tccctataaa
     6601 actatttcta ttggccataa atggaggtaa gacttccgta tagccatgct tagctgtatg
     6661 agtatttaaa aagaaattca ttaaagatct ttctaatcta gctcctaatc ccctatataa
     6721 agtaaacctt gacccagtta ttttccctgc tctttcaaaa tctaaaatac ctagacctgt
     6781 acctatatcc caatgcgctt tatgctcaaa atcaaattta gtaggttctc cccaagttct
     6841 tatttgaaca ttgtcttcat ctgtttcacc ttgtggtacc tctggatgag gaacatttgg
     6901 tattctcatt aatgtatatt ccatttttgc ttctacttct tttactttct catcaatacc
     6961 ttttatctta tctgataact ctttcaatct tgccttttct tctgttacat cttttccttc
     7021 ttttattaat tgaggtattt ttttagaatt tacatttaac tcatttttca taacttcaac
     7081 ttcttgaagt atttctcttc ttttattatc taattccact acagcatcaa ggtcaaattc
     7141 tctttcacct cttctttcca tagctttttt gatatcatct agattttctc ttattctttt
     7201 aatatctaac atattaattc ctcctaataa ttttgataat ataagttata attccaactt
     7261 aatttacaaa cattatactt gtaaactctt agtttaaaca aattttaaaa ataaaaaaag
     7321 acctccgttt accctataaa agggacgaaa atcttttatc cgcgttgcca ccctaattga
     7381 ctatactaaa atgtataatc ctctcagacc tataacgtag gtaaacgtct agctttaact
     7441 agaagctcta gagtagattc aaaaaccatt gtaatgagtt ttcaccatcc actcattctc
     7501 tgaatacaat ctgtttttta ctattttcct tcacagcttt ttaactaatt gttttatttg
     7561 ataatcatta tattatatat attaacatct ttcaatacta ttattcaaat ttttttagaa
     7621 ttttctttaa ttttttatat taaattcatt ttttctattt aattaagacc ttatctttta
     7681 tccattgtat ttagattacc tgtattatta aataataatg aagaaggaac aaatggaact
     7741 tttatatcat tctcatcaaa tttatttttt accttttcca ttacatcaaa atatacatcc
     7801 cagtaatcat cagtttttac ccaaggtcta gcaactattt gcatggtgtt atttcctata
     7861 gagtttagac ctattatagg ctttggatca tttaatattc ttttttcgtt tgtaaaaatt
     7921 tcatttaata tagacttaac tacatctata tcactatcat atgaaatatt atatgaaaaa
     7981 tctattcttc ttttgttttg atgtgtgtaa ttaattatat tattacttgt cagttgaaaa
     8041 ttaggtataa ttatagtttt attatctggc gtcttaagaa cagtgctaaa tatttgtatg
     8101 tcagaaactg taccttctac acctgaacct tgaatataat cacctatatt aaatggctta
     8161 aaaaataaaa taatcatacc agagcctaaa tttgataata tttctttaaa agctaaacct
     8221 atactaaatc cagcagctcc taacacagct acaactgatg tagtttgtat ccctataata
     8281 cctaagctta tcaatgatat aatagataac atagatatat atattccata tacaataaaa
     8341 ttatttacac ctttactcat tccatatttg attagaaact ttgaaaccat ccttcttact
     8401 attttagata tataaatacc tatcacaaat acaattattg catatataat atttggtata
     8461 ttatcactaa aattatcgag catattttct aaagttctct tagttatatt gctatttgtt
     8521 gaggcagtct tagccaatga taaaaaattc attcaactcc tccttatgat aatattccgt
     8581 tgtcctttaa ctcttttact atagcttcta tagtttcttc ttttattgcc tcaattgtat
     8641 gaatatggct acattctgtc agaaccgata attgtttaaa ttcatttgtc tcaactttct
     8701 tcataaagtt gtctatatcc attttagaat aaatattaag ttctgcagtt atctgaccat
     8761 atgttggatg atttacgatg acatctttta taataccacc taaatccaca attatcttta
     8821 attcatcata aatttcttta gaatccttat gatttttaca ctttatttgt tttgtaaaat
     8881 acggttgatt atatattata tatccttgag gtgtagctat tatttcaaat cctctagctc
     8941 ttataagggc tatatcttga actattactt gcctacttac atttaatagt tcagctagtg
     9001 ttccaccttt tacaggatgc ttagattctt ttaaaatatc tattaattta cttcttcttt
     9061 cattagaatt catattacac cttctttcca aataaaaact ttacctatac tatataattt
     9121 tatattttat cttattataa atcaatattg actttatatt ctatttaatc ttaactttta
     9181 taataaataa aaaaagacta cgtggctacg tcctactctc ccaggcagtt tcccaccaag
     9241 taccatcagc gctagagagc ttaacttctg tgttcggaat gggaacaggt gtatcctctc
     9301 tgctattgta accacataat ctttatgctt aatatttcta ttaagttttt aataagttta
     9361 tagaatctag taatactagt ttctttagag ttagcactct aaaaactaca catatatctt
     9421 aat
//
LOCUS       DAEMHW010000143         9416 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEMHW010000143 DAEMHW010000000
VERSION     DAEMHW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.143"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1037
                     /locus_tag="KPK67_002821"
     CDS             <1..1037
                     /locus_tag="KPK67_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG2459759.1"
                     /translation="LSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITNEY
                     IENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYKAL
                     EYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQTRR
                     ICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMALQ
                     YVNKQTDKICIEAVKQDGRSMQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYTED
                     ICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKAKE
                     YVKIAIDDCI"
     gene            1176..1628
                     /gene="tsaE"
                     /locus_tag="KPK67_002822"
     CDS             1176..1628
                     /gene="tsaE"
                     /locus_tag="KPK67_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBG2459760.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            1628..2344
                     /gene="tsaB"
                     /locus_tag="KPK67_002823"
     CDS             1628..2344
                     /gene="tsaB"
                     /locus_tag="KPK67_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBG2459761.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            2334..2810
                     /gene="rimI"
                     /locus_tag="KPK67_002824"
     CDS             2334..2810
                     /gene="rimI"
                     /locus_tag="KPK67_002824"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBG2459762.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            2816..3832
                     /gene="tsaD"
                     /locus_tag="KPK67_002825"
     CDS             2816..3832
                     /gene="tsaD"
                     /locus_tag="KPK67_002825"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBG2459763.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            4129..6837
                     /gene="hpdB"
                     /locus_tag="KPK67_002826"
     CDS             4129..6837
                     /gene="hpdB"
                     /locus_tag="KPK67_002826"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBG2459764.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            6841..7098
                     /gene="hpdC"
                     /locus_tag="KPK67_002827"
     CDS             6841..7098
                     /gene="hpdC"
                     /locus_tag="KPK67_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBG2459765.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            7091..8041
                     /gene="hpdA"
                     /locus_tag="KPK67_002828"
     CDS             7091..8041
                     /gene="hpdA"
                     /locus_tag="KPK67_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBG2459766.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRLPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWTQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            8356..9318
                     /locus_tag="KPK67_002829"
     CDS             8356..9318
                     /locus_tag="KPK67_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2459767.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNINN"
BASE COUNT         3683 a          980 c         1783 g         2970 t
ORIGIN      
        1 atttatctaa aaaaataaaa aaattatttt tagatgataa agggtattat tttaatagta
       61 attggaaaga tattcatatt ggtatttatg atacaaaatt aggagattct ttaaagtata
      121 tcactaatga atatattgaa aatatggaaa gggtaaaata cgattatcgt gaattaaaaa
      181 atatagaaaa tcaaactgat ggtatgtgta ttttagcaat aaggcagaat tacaaggcat
      241 tagagtatgt aaaaaatcaa accgaagaat tatgtttaga agcaataaag tataattata
      301 aagcacttga atatgtaaaa gaacaaacag aatacttgtg tcttgaagct ataaaaaaag
      361 attgtaatgc acttaagtat gtacgaaata aaacagaagg gcttattata aaagcaataa
      421 gtcattcttc caatatagat gttgtaagta tactaaaggc tttagagact caaacaagaa
      481 ggatatgttt agaagcaatt aaaaaagatg gcaggtgtct tgcatacgtt agagaacaga
      541 gtgaagaatt atgtatagag gccattaagc aaaattataa agcattaaaa tatgtaaaaa
      601 atcaaacgga aaaaatgtgt atagaatccg taagacaaaa tggtatggca ttacaatatg
      661 taaataaaca aactgataaa atatgtatag aagctgtcaa acaagatgga agaagtatgc
      721 aatttgtcaa caataagaca gaagaaatat gtataaatgc tataagatat ttaaataaaa
      781 aatataatat taaagatgta ctaagttaca tagataagta tacagaagat atatgtatag
      841 agattgtaag acaaaatgga aaaatgctta tgtatataaa aaatcaaaca gaaaaaatgt
      901 gtattgaagc agtaaaagag aactataagt ctcttaagta tgtaaaagag caatctgaga
      961 gaatatgtaa agaagcattg aagcaaaacc ataaagcaaa agagtatgta aaaattgcta
     1021 ttgatgattg tatataaggt gtagatacag ccatcaatag tgaaagataa aaaaagataa
     1081 agacaagtac tttataataa tctaggctta tactataatt aattaagcag tgatataaat
     1141 tcacatgacg attatatcaa aatggagatg aattgatggc aaaaatatat ttagaaaatg
     1201 aaaataaaac tagagaaata ggatataaat taggtaaact tttaaaagaa ggcagtgtta
     1261 tctgtttagt aggagattta ggagctggta aaactacaat gacacaaagt ttggcagatt
     1321 cattagggat agaagattat ataacaagtc ctacttttac tataataaat gaatatgaag
     1381 gaaaaatacc tttgtatcat tttgatgttt atagaatagg tagtagtgat gaaatgtatg
     1441 atattggtta tgatgaatat gtaaattcaa atggaatctg tataatagaa tgggctaatc
     1501 taatagaaga tatacttcca aaagaatatt tgaatataga attgagatat aaagatgaag
     1561 gaagagaaat gatattaact ccaaaaggag agttttataa agagatagtt gaggagttga
     1621 ttaaataatg aaaatactag gtatggatac gtcttctatg gcagcaagtg tagcagttgt
     1681 tgaagatgat aacttaatat gtgagtttac agtaaacaac aaaaaaactc attcacaaaa
     1741 gcttatgcca atgatagaaa atatgttgag tatgagtgat ttaagtataa aagatatgga
     1801 tttattagct gtatgtatag gtccaggttc gtttacagga cttagaatag gtatggctac
     1861 agtaaaagct atggcccatg taaataatat accaataata gcagtaaatt ctttggaatc
     1921 tctagcaaat aacataaatt tctgtgatag aaagatatgt tgtatattag atgcgcagaa
     1981 aaatcaagtt tattcagcta aatacaagtt tgaaaatgga gagatgatag aattagatgg
     2041 tgttgatgtt gtagagtttg atgctttagt agatgaaatt gtatcaacta atgaagaatt
     2101 tataatagtt ggagaagctg tatacaaata taaagacaag ttagagaata taaaaaatat
     2161 aaaaatacct tcaccagcta ataatgtatc taacgcagga agcttatgct ctttagcatt
     2221 aaataaatat aataaaaata tagatgtaca tacttgttat acgataaatc ctatgtatat
     2281 cagaaaatct caagctgaag tacaatatga tgaaaaaatg aagaggctaa ataatggaag
     2341 ataatacaaa actaatagta gatgatataa aaatagaaga aatgactaca gaagatatag
     2401 atgaagtttt tgaagtagaa aaaaattgtt ttgaagatta ttggtcaaaa gagtctttta
     2461 gaaaagagtt gtctaatgaa gtagcaaggt atatagtagc taagttaaat ggaaaaattg
     2521 tcggatatgt gggaatttgg cttatacttg atgaagggca tataaataat gtggctgttc
     2581 atagtgacta tagagggaaa aaaatagggg acaagttgat aaaaggcata gttgatttat
     2641 gtaaagataa taatattgca tctatgacat tagaagttag agcatcaaat aaaatagcac
     2701 aaaatttata tagaaaatat ggatttaaaa tgggtggaat aagaaaagaa tactacagtg
     2761 ataataaaga agatgcaatt ataatgtgga accaattaaa agaggtgtag agtttatgag
     2821 tgatataata acattagcta tagagagtag ttgtgacgaa acagctgcat cagttttaaa
     2881 aaatggtaga gaagtacttt caaacataat ctctactcaa atcgagacac ataaaaaatt
     2941 tggtggtgta gttccagaag tggcctcaag aaaacatgtg gaaaatattg atatagttgt
     3001 tcaagaagct ttagacaaag caaatatagg ttttaatgat atagaccata tcgcagttac
     3061 atatggacct gggctagtag gtgctttatt ggttggtcta tcttatgcta aagcacttgc
     3121 atatactttg aatattccac ttgtaggagt aaatcatata gaagggcatc taagtgcaaa
     3181 ctatatagag cataaggatt taaaacctcc ttttataaca ttaatagtat ctggaggtca
     3241 tactcacttg gttgaagtaa aagattatgg caagtatgaa attttaggaa aaacaagaga
     3301 tgatgcatct ggagaagcat ttgataagat ttcaagagct atgaatttag gctatccagg
     3361 aggccctatt attgataatc tggccaagaa tggaaataaa catgctatag aatttccaag
     3421 agcctattta gaagaagata gctatgattt tagttttagt ggattaaaat cttctgtatt
     3481 aaattatcta aatggaaaaa gaatgaaaaa tgaagaaata gtagttgaag atgtagcagc
     3541 atctttccaa gaggctgtag tagaggtttt atctactaaa gcactaaaag cagtaaaaga
     3601 caaaggatac aatatcataa ctctttctgg tggggttgct tctaattcgg ggcttagagc
     3661 taaaataaca gagcttgcta aagacaatgg tattactgtt aagtatcctc ctctaatttt
     3721 atgcacagat aatgcagcta tgatagggtg tgcaggatat tataatttta taaacgggaa
     3781 aacgcatgat atgagtttaa atgcagttcc aaatttaaag ataaatcagt agagttagat
     3841 gaatttgtaa atataaaagt attgtttcaa aaaagtaagt agaagtgaaa ttaaatataa
     3901 taaaaataaa tatagtatat attaaaactt gaagtaaaac tatttataat ctttatattc
     3961 caatgaattg caacaaattt taggatatac agattgtctt tggcttagat ttgcttcaag
     4021 tttaattttt tctatagaaa tattatttta aaaagtgggt aatataccct tttttttcat
     4081 tcttaaaaag ttttatacta attatagaaa gattaaggag gggaagaaat gagtcaaagt
     4141 aaagaagaca aaataagaag tattttggaa gcaaaaaata ttaaatcaaa ttttcaaaat
     4201 aaagaaaatt taagtgaatt taatgaaaaa aaagcaagta aaagagcaga agacttatta
     4261 gatgtatatt ataatacatt atctactgct gatatggaat ttccatattg gtataataga
     4321 gaatatagaa aatcagatgg tgatatacca gtagttagaa gagctaaagc tcttaaagct
     4381 gctttttcac atatgacacc aaatataata cctggtgaga aaatagtaat gcaaaagaca
     4441 cgccattata gaggttcttt tccaatgcca tgggtaagtg aaagcttctt tgttgctcaa
     4501 ggagaacaaa tgagagaaga ggcaaagaaa ttagccagta acacagctga tgaacttaca
     4561 aaatttgggt ctggtggagg aaatgttact gaaagttttg gaaatgtcgt ttcaatagct
     4621 ggtaaattcg gaatgagaaa agaagaagta cctgtacttg taaaaatggc aaaggaatgg
     4681 gttggaaagt ctgttgaaga tttaggtttt cactatgaaa aaatgatgcc agattatgat
     4741 ttaaaagaaa acctaatgag tacacttata tgcatgtttg attcagggta tacacttcct
     4801 caaggtagag aagttataaa ttatttttat cctttaaatt atggattaga tggaattata
     4861 gaaatggcaa aagaatgtaa aaaggcagtt gcaggaaatg catctggaga tgggcttatt
     4921 ggtatggacc gtctatattt ttatgaggct gttattcaag ttattgaagg attacaaact
     4981 tggattttaa attatgcaaa acatgcaaaa tatttagaaa gtatagagac agatttagaa
     5041 gctaaaaaag aatactcaga tttagtggag atattagaac atattgcaca taaacaacct
     5101 cgtacattta gggaagctct tcagttgact tatacaattc atatagcatc agtaaatgag
     5161 gatgctatat ctggtatgtc tataggacgt tttggtcaaa ttttatatcc ttggtatgaa
     5221 caagatatag aaaaaggact aattacaaaa gaagaggtta tcgaattatt agagctttat
     5281 agaataaaaa tcacttgtat tgactgtttt gcttcagcgg gcgtaaatgg aggagtacta
     5341 tcaggcaata catttaatac tttatctata ggtggattaa aagaagatgg ttctacagga
     5401 gccaatgaac tagaagagtt attattagaa gcaagtatga gatgtagaac tcctcaacct
     5461 agtttaacta tgctatatga tgaaaagctt ccagaagatt tcttaatgaa agctgctgaa
     5521 tgtacaaagc ttggttctgg ttatccagcc tgggtaaaca actcaaatgg aacaacattt
     5581 atgatgaaac aatttgctga tgaaggtatg actgttgaag aagcaagagc atttgcatta
     5641 ggtggatgtt tggaaacgtc tccagggtgt tggaaacagt taactttaaa cggaaaaacg
     5701 tactctattg caggtggagc aggtcaatct gctggctctg gagttcactt tatagctaat
     5761 cccaaaattt tagaacttgt tcttatgaat ggtaaagacc atcgtatgaa tatacaagta
     5821 tttgagcctc ataataagcc attagatact tatgaagaag ttatagaagt atttaaagat
     5881 tattataaac aagcaataaa tgttttagaa agagcaaata acattgagtt agatatatgg
     5941 agaaaatttg atacttctat aataaattca ctattaaagc cagattgttt agacaaaggt
     6001 caacatatag gaaatatggg gtatcgttat aatgcaacat taaatgtaga aacttgtggt
     6061 actgttacta tggtcaattc atttgcagca cttaaaaaac ttgtatatga tgacaaagcc
     6121 tttacaatag aggaaatgaa ggatgccata ttaaataatt ttggatttaa agacgcatta
     6181 gaagtaggaa attactctat ggcagaccaa gtaaaagtag ataagactgg taagtatgat
     6241 gctatttata aggcctgtct tgatgcacct aaatatggaa ataatgattt atatgcagat
     6301 aatattctta aaaactatga ggtatggtta tcaaaagtat gtgaagaagc acaatcttta
     6361 tatgctaaga agatgtaccc ttgccaaata tctgtttcta cacatggacc acagggggcg
     6421 gctactttag ctactccaga tggtagacta agtggaacaa catattcaga tggttcagtc
     6481 tctgcatatg caggaacaga taaaaatgga gtatatgcat tatttgaatc tgcaactatt
     6541 tgggaccaag cagtagttca aaattctcag atgaatttaa aacttcatcc aactactata
     6601 aaaggacaac aaggaactaa aaaattatta gatttaacaa gaagttattt aagaaaaggt
     6661 gggttccata ttcaatataa tgttgtagat tcagaaacat taaaagatgc acaaaaaaat
     6721 cctgacaatt atcgtcaatt aatggtacgt gttgctgggt ttactcaata ttggtgtgaa
     6781 ttaggtaaac caatacaaga tgaagtaatt gctagaacag agtatgaagg ggtgtaaaat
     6841 atgagaaagc atagtgattg tatgaatttt tgtgcagtag atgcaaccaa aggaatttgt
     6901 agattatcaa aacaaatgat taatttagat gatgcagcat gtccagagat aaaagtaatg
     6961 ccaaaatgta aaaattgtaa aaattttgtt gaagctaatg atgaaggtat aggcaaatgt
     7021 gttggcctag agaaagaaga ctgggtatat tcaacattga atgcaattac ttgtgaagga
     7081 catgtgttta atgagtagtc aaaagcaatt agaaggcatg attttcgatg tacaaagctt
     7141 ttcagttcat gatggtccag gttgtagaac aactgtattt ttaaatggat gtccactgag
     7201 ttgtaaatgg tgtgcaaatc cagaaagttg gactgtaaga ccacatatga tgtttagtga
     7261 attatcttgt caatatgaaa atggatgtac tgtatgtcat ggtaagtgta aaaatggtgc
     7321 attgagcttt aatcttgata ataagccagt tatagattgg aacatctgta aggactgtga
     7381 aagttttgaa tgcgtcaact catgttatta taatgcgttt aaattatgtg caaaacctta
     7441 cactgtagat gaacttgtgc aggtaattaa acgtgattct aacaattgga gaagtaatgg
     7501 aggagtaaca tttagtggag gtgagccact acttcagcat gaatttttac atgaagtatt
     7561 attaaagtgt catgaggtaa atatacacac tgccattgaa acgagtgcat gtgtatcaaa
     7621 tgaagttttt aataaaatat ttaacgatat tgattttgca tttattgata taaagcatat
     7681 ggatagagaa aaacacaaag aacaaacagg tgtgtataat gatttaatat tagaaaatat
     7741 atcaaacctt gcaaattctg attggaatgg aagattagta cttagactac cagttatctc
     7801 tgggtttaat gatagtgatg aaaatataag tgatattata tcttttatgc acaaaaataa
     7861 tttggtagaa attaatctat taccatttca cagattagga gaatcaaaat ggactcaatt
     7921 aggcaaggaa tatgagtatt ctgataaggg tgatgttgat gaaggtcatc ttgaagaatt
     7981 acaagatata tttttggata atggtatagc ttgttatgtt ggtcatgaga cagctttcta
     8041 aaattaaata caagttttaa ttaaaaaagg atgttacact aaaagctaga gttacatcct
     8101 tttttatgtg gtttttatgt agaaataatt aagtaaagtt ttatatgttt tatatattgt
     8161 ttatattgct ttatgtccat aaatattgct ttaaatatac tttatatatt ttttatagtt
     8221 taaattacag ttagagaata tattacgttt acttcattga agcagaagtt ttgtggtaaa
     8281 atactactat gaaaaaattt aatagaatat attttagaaa caactaaaaa taatagttag
     8341 gaggaagaca tctatatgaa caaatcatgg agcaacttga ttattaagaa taaagacaaa
     8401 aaagtacgtt cagggtggat tattatagct gttatggcta ccttttacat acttcagtat
     8461 tatttaagca tgatacttgt ggaaataata agaaaaatcc tggttgctac aggtgacatt
     8521 aatttaaaaa caaactattt ttcagaatat gctaattggt taaatgatgt tgggttacag
     8581 attgtatttc aaattctaac agattcactt atgattatta ttcctataat tgtatggaga
     8641 tttattatga aaaatcctat tagtgaaatg ggattacatt cttatagaat taagaaaaaa
     8701 gaaggatatg taggaatgtt attaggaata tttaattgta cagtaatctt tattataaca
     8761 ataacaattg gtggtggtca tgttacttca tgggtaccaa aaatttcacc tttaacagtt
     8821 tcttggatac ttgtctttat tttagttgct tttgcagaag aaattttaaa tcgtggtttt
     8881 tttatggcag ttttacgaag atgtaaaaat atttatttta taatgattgt tccatctgtt
     8941 atttttgggt tgattcatat atggaatcct gatgttactt ttttgtctgt aattaatatt
     9001 ataattattg ggattctttt ttcatatatg tttataaagt cgtcgaatat atggatgtgt
     9061 ataggttatc attttacatg gaatgtattt caggggatta tatatggtat gcctgtaagt
     9121 ggacttcagg ttccagggat tataactaca caaatcactc atggtaatct attaaatgga
     9181 gggatgtttg gtattgaagg tggaattttg actacttttg ttaatctact tagctttata
     9241 tttgtatggt attactatag agattctaag tatgattttg ttagtgataa agtaaatgtg
     9301 accaatatca ataattagaa agattatgaa aactaaatta gaagaactaa aaactatatt
     9361 tttattaatt ataatatttg aataatctct ttaatcatat aaaatgcata tacttg
//
LOCUS       DAEMHW010000144         9389 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEMHW010000144 DAEMHW010000000
VERSION     DAEMHW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.144"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            144..695
                     /gene="raiA"
                     /locus_tag="KPK67_002830"
     CDS             144..695
                     /gene="raiA"
                     /locus_tag="KPK67_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG2459768.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGGENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            827..3502
                     /gene="secA"
                     /locus_tag="KPK67_002831"
     CDS             827..3502
                     /gene="secA"
                     /locus_tag="KPK67_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG2459769.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            3517..4624
                     /gene="prfB"
                     /locus_tag="KPK67_002832"
     CDS             join(3517..3591,3593..4624)
                     /gene="prfB"
                     /locus_tag="KPK67_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBG2459770.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKNLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            4768..6909
                     /locus_tag="KPK67_002833"
     CDS             4768..6909
                     /locus_tag="KPK67_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBG2459771.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDSREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            7057..8223
                     /locus_tag="KPK67_002834"
     CDS             7057..8223
                     /locus_tag="KPK67_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2459772.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      8283..8400
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            8535..9140
                     /locus_tag="KPK67_002835"
     CDS             8535..9140
                     /locus_tag="KPK67_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2459773.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
BASE COUNT         3922 a         1011 c         1804 g         2652 t
ORIGIN      
        1 ctaaaaatga taaataattc tatcaattga atagaaaaaa tcataaaagt aaataataaa
       61 aagaggatta tgagagttcg tgtagaatat attattaaag tataaatttt cttatactgg
      121 cataattgaa gggagatgta tttatgaaca taattatatc tggaaaacaa atcgaattaa
      181 ctgatgcaat aaaaaataaa atcgaaacta aactaagtaa gttagataat tacattcatc
      241 cagacactga tgtaaaagtt acagtaagtg cgagaaaagc aagacaaaaa atagaagtaa
      301 caatagctac tgtaaatggt cctataatta gagcagaaga tatgcaggaa aatttataca
      361 cagctataga tgttgtatat gataaattaa gtaaacaatt aaaaaaatat aaaaagagat
      421 tacaagataa acataaagac aacaagagta taagattcca agatgcagag ataaatctag
      481 ctgatgaaca agaggattat gggggtgaaa atcaagaatt agtaattcaa agacgtaaga
      541 aatttagtgt taagccaatg agtgaagaag aagctgtatt acagatggaa ttaataggac
      601 atgattttta tatgttcaag aacatagatt cagatgaaat agcagtagtt tataagcgta
      661 ataatggtgg atatggtata atagaacatg aataaagcaa tttagattta atcatattag
      721 ctatcgataa ataaattatc gatagctttt taaattaata ttaaaagtat agtttttatg
      781 gtaaaatgaa taatagtaca ataaagaacg aggagagatg taaaatatga gctttatgga
      841 taatttattc aacatggcag ataaaaaaga attaaaaaag ttcaataaaa ctgttgatat
      901 aatagattca ttagaaccta aatttgaatc tatggcagac tcggaactta aaaatatgac
      961 aaatatattt aaagaaagat tagcaaatgg tgaaagtata gatgatatac ttccagaagc
     1021 ttttgcagta gttagagaag tttctaagag agtgttagga cttagacact acagagttca
     1081 aatgattgga ggtatagtac tacatcaagg aagaatagca gaaatgaaaa caggtgaagg
     1141 taaaactttg gttgcaactg ctcctgttta tttgaatgcg cttactggaa aaggtgtaca
     1201 tgttgtaaca gtaaatgatt accttgcaaa acgtgataga gaccaaatgg caaaaatcta
     1261 tgagttttta ggaatgagtg taggcgttat tatacacggt caaaatccaa aagttagaaa
     1321 agaacaatat gattgtgata taacttatgg tacaaataat gaatatggat ttgactatct
     1381 aaaggacaat atggtaatac acaaagagca aagagttcaa agaggattaa attatgctat
     1441 agtggatgag gttgactcta tactaataga tgaggctaga acaccactta tcatatctgg
     1501 acctggagac aaatcaactc acttatattc agatgcaaac acatttgttt taaccttaaa
     1561 accagatgat tatgaattag aagaaaaaga taaggctgta tctctaacag catcgggtat
     1621 acaaaaagca gaagtgtatt ttaatgttga taatataaca gatatatctc atacagaatt
     1681 ataccatcat ataaatcaag ctttaagagc acatgtaata atgaagaaag atgttgatta
     1741 tgttgctaaa gatggagaaa tagtaatagt agatgagttt acaggtagac ttatgtttgg
     1801 tagaagatat tcagaaggtt tacatcaagc aatagaggct aaagagggtc ttaaaataca
     1861 aagagaatct aaaacactag ctactgtaac attccaaaac tactttagaa tgtacaaaaa
     1921 actttctggt atgactggta cagcaaaaac agaagaagaa gagtttaaag caatctacaa
     1981 aatggatgtt ttccaagtac ctactaataa gcttatgata agggaagact taccagattg
     2041 tgtatacaaa agtgaaatag gtaaatttaa tgctgtagca caagaaataa ttgaaaggca
     2101 caaagtcaat caaccaatac ttgtaggtac agtatcaata gaaaaatcag aattattatc
     2161 acaaatacta aagaaaaagg gcataaagca cgaggtatta aatgctaagc atcatgataa
     2221 agaggcagaa ataatagcac aagctggtag acttggagct gttacaatag ctactaatat
     2281 ggctggtcgt ggtacagata tagttctagg tggaaatcca gactttttaa ctaagagaga
     2341 aatgagaaga aatggattta aagaagaaat agtaaataga gtagatactc caatagaagg
     2401 tatacctgta aaaggtaatg aaattctatt tgaagctaga gaagagtatg aaaaactatt
     2461 tgaaaagttt aagcaacaaa cacaagaaga acaaaaacaa gttgtagaag caggtggact
     2521 tgctataata ggtacagaaa gacacgaatc tcgaagaata gataaccagt tgagaggtcg
     2581 tgctggacgt caaggtgacc caggtagttc aagattctat ataggtcttg atgatgacct
     2641 tatgagattg tttggtagtg atagaatatc tggtattgta gataaaatag gtcttgaaga
     2701 agacatgccg atagaacata gaatattatc taagtctatt gaaggagcac aaaagaaagt
     2761 cgaaggtaaa aactttggaa taagaaagca tgtacttcaa tatgatgatg ttatgaataa
     2821 acaaagagaa ataatatatg cagagagaaa acgtgtatta gaaggtgaag atttacaaga
     2881 acaaatacaa tcaatgactc attctataat agaagaagca gttactcttt atacacaaga
     2941 taaaggattt gatgaagaag gatttaaaga acatatgtat aatttgttct tgccaaaagg
     3001 tagcatagaa ataccagaaa tagaaaaact taatccagtt gaaataacag aaaaagttta
     3061 tgaaatagct atgaagatat atacttcaaa agaagaacaa gttggctatg agagaatgag
     3121 agaagttgag agagtaattt tacttcaagc tgttgataac cattggatag accatataga
     3181 tgctatggac caattacgcc aaggtattgg tcttcgtgct gtaggtcagc aagacccagt
     3241 tattgcttat aaaatggaag gttttgatat gtttgatgaa atgaataaac atatcaaaga
     3301 agatactgta agatatttat ttaatataac aatagaaact ccagtggaaa gaaaagcagt
     3361 tgtagatgtt gaaaatctat cttctccatc agatggaaca cttccaacaa gtaaaactgt
     3421 taaaaaagat gaaaaggttg gaagaaatga cttatgccct tgtggaagtg gtaaaaaata
     3481 taaaaactgt tgtggaagat aatctcggag gattaaatgt taaagttaca agagtataga
     3541 catagtatgg agcagatggc ttcaaattta gaagaattga gggtttctct ttgacatagc
     3601 ttcattgatg aaaaatatac aaattaatga agaaaagatg aatcaacaag acttttggaa
     3661 tgataatgaa gtcgctcaaa gagtacttca agaaaataaa tctttaaaag agacattaga
     3721 agaatatgaa agcttaaaaa gcttattaga agatatagaa gtattaattg aaataggttt
     3781 agaagaagat gatgattctg tagaaagaga tatagaaaag tccatagaat caatggaaga
     3841 aaaattatct gaaatgaaaa taaagaatct tctaaatgga gagtatgata aaaataatgc
     3901 tatattatct ataaatgctg gtactggtgg cttagatgct caagattggg ctcaaatgct
     3961 tttgagaatg tatataagat ggtcagaaag taagggttat aaagttaagc ttttagatat
     4021 aatatcagac cctgaagcag ggataaagac agctacaatt cttgtagaag gaacaaatgc
     4081 ttatgggtat ttaaagagtg aaaaaggagt tcatagatta gtgagaatat ctccatttga
     4141 cccatcagga aaaagacata cttcatttgc atctatagat gtaacaccag agctagatga
     4201 aaatatagaa gtagaaatta atccatcaga tttaaagata gacacatata gagcttctgg
     4261 agctggaggg caacatgtaa atacgactga ttcagcagtt agaataacac atatccctac
     4321 tggtgtagta gttcagtgcc aaaatgaaag gtctcagcat ctaaataagg acagagctat
     4381 gagactttta atggcgaaat tgatagaatt aaaagagtta gaacaaaagg aaaaaataga
     4441 agatatacaa ggcaaatact cacaaataac ttggggtagt caaattaggt catatgtttt
     4501 tcaaccatat aaattggtta aagaccatag aacaaatgct gagtttggaa atgtagatag
     4561 tgtaatgaat ggaaatatag acttgtttat taatgaatac ttaaaaatga ataaaatagt
     4621 ttaataaata tgcactgtaa ttaagatata tgtataagaa ggttagtgct taatatgcac
     4681 ttacagcact taggctgagg acgataaccc tcagccttcg ttctgttttg ttatacttat
     4741 gaaaaactaa cattaggagt gaataaattg gatataaatc aaatcttaaa aaaagagttt
     4801 aatcttagag atgaacaaat aaataataca ttaaaactta ttgatgaagg aaatacaata
     4861 ccatttatag ctagatatag aaaagaaatg actggcgaaa tgagtgatgt tacactaaga
     4921 gaattttatg aaaaattaat gtatttgaga aatcttcaaa gtagaaaaga tgatgtagtt
     4981 agactaatag atgaacaagg gaaattaaca gatgaaataa cacaaaatat agaaaaagca
     5041 aaaacattac aagaagtaga agatatatat gctccatata aacaaaagaa aagaactaga
     5101 gcaacaatag caaaagaaaa aggattagaa aatttagcat tgtcaatact agagaataat
     5161 ttagataata tagaaataga ggcaaagaat tatcttgatg aagagaaaga agttctttct
     5221 attgaagatg ctctaaaagg tgctagagat ataattgcag aactagtctc agatgatgct
     5281 aagattagaa agtatataag agaattagct ttaagagaag gtatgatagt tagcaaaagt
     5341 gctactgatg agaaatcggt ttatgatatg tattatgatt atagcgaagc agtaaaaagt
     5401 atggctccac atagagtttt ggcaataaac agaggagaaa aagaaagctt cctaaaggtt
     5461 aagttagaaa taaataatga taaagtttta aattacataa taaacgaata tgtaaatgat
     5521 aaaaatttca aaaataaaga agaaatcgta agttcaatag aagattctta taaaagatta
     5581 attttcccat caatagaaag agaaataaga aatcatttaa cagaaatagc tcaagaaaga
     5641 gctataagtg tatttggaaa gaatgttaaa agtttattgc ttcaaccacc agttaaagac
     5701 aaagtagtta tgggatttga ccctgcattt agaactggat gtaaaatagc tgtagtagat
     5761 aaaaatggta aactactaga ttatacaaca gtatatccaa cagaccctca aaatgatgta
     5821 gagggagcaa agaaagtttt aaaaggattg attgaaaaat acgatataga tataatttca
     5881 attggaaatg gtactgcatc tagagaatca gaaacttttg tatcagaaat gataaaagaa
     5941 atagatagcg aagtgcagta tgtgatagtt agtgaagcag gagcatctgt ttattcagca
     6001 tctgaacttg caaatgagga acatccagat ataaatgtat caataagagg agctatatct
     6061 atagctagaa gattacaaga cccattagca gagcttgtaa aaatagaccc taaaagtatt
     6121 ggtgttggtc aatatcagca tgatttaaac aaaaagagat tagaagaagt actagatgga
     6181 gttgttgaag attctgtaaa tagtgttgga gtagatttaa atactgcatc atactcccta
     6241 ctagaacatg ttgcaggaat aagtaaagct atagcaaaaa atataatagc atatagagaa
     6301 gaaaatggag attttacatc aagagcacaa ttgaaaaaag taaaaagact aggacctcaa
     6361 gcatttactc aatgtgctgg atttatgaga atcttagagg gtaaaaatcc actagataat
     6421 acaggtgtgc atcctgaaag ttatgatata tgtaaaaaaa tgattgagat aattggatat
     6481 tctttagatg atgtaaagaa taagaatata ggtgaaatag atgaaaaaat aaaagaaatt
     6541 ggcttgaggg aattaagtga aaaactggaa gtaggacaag ttacgttaaa agatatcata
     6601 gctgaaatca aaaaaccagg aagagactca agagaagaag gtataaaacc tatacttaga
     6661 actgatgtct taaaaattga agatatacaa gagggtatga ctttaaaagg tacaataaga
     6721 aatgttgtag actttggtgc atttgtagat attggaataa aaaatgacgg attagttcat
     6781 aaatcagaaa tgagtaatag ttttgtgaaa gaccctatga gtattgttac agtaggtgat
     6841 atagtagatg taaaagtcat tggaattgac ttaaacaaaa aaagagttgc cttatcaatg
     6901 aaaaaataga taagtttatg tatggttata gtacttagat tctatatttg taaatgagat
     6961 tactcgagtg tataaatgtt aaaaatttgt aaaagtatat agtaagaaaa tatattaaaa
     7021 taagaacgat agtcttgaaa atcaggagga aaatgaatga tttttataaa aaatggtaaa
     7081 ataaatacca taacaaatgg gataattcat ggagatatac ttatagatga aggaaaaata
     7141 atagaaattg gagaagattt gatagctcct ttagatgtag aggtaataga tgctagtaat
     7201 aaacttgtgt ttcctggatt tatagatgct catactcact taggtttatg ggaagatggt
     7261 atagggtttg aaggagcaga tggaaatgaa gagactgacc caataacacc acaattaaat
     7321 cctatagatg gaattaatcc aatggataga acttttaaag aagcttttga aggtggaata
     7381 acttctgttt gtacaacccc tggaagtgca aatgttatgg gtggtcaatg tatagctata
     7441 aagacatgtg gaaaaagaat tgataaaatg gtaataaaaa atccagttgc atctaaaatt
     7501 gcttttggag aaaatccaaa gagctgttat ggtcaggatg ataaatctcc tcaaactaga
     7561 atggctatag ctgctttact tagagaaaac ctaaaaaaag cagaagaata tttagaggat
     7621 attgatatgt atgagagtca tgatgatgaa gactgtgaaa aacctgaata tgatataaaa
     7681 atggaaagtt taataccagt tttaagaaga gaaattcctt ttaaggcaca tgctcataga
     7741 gcagatgata tgtttacagc aataagaatt gcaaaagaat ttaatttaaa gcttacatta
     7801 gaccattgta cagaaggtca tcttattgta gatgaattag tggaagaagg gtttcctgta
     7861 atagtagggc catctttgtc agaaaggtct aaatttgagc ttagaaattt gacatttaat
     7921 acagcaggaa tactttcaaa tgcaggtctt gatgtatgta taatgacaga ccatccagta
     7981 ataccagttc aatatcttcc aatatgtgca ggtattgcag taaaacatgg tatgaaagaa
     8041 gaaaaagcaa tagaatcaat tacgataaat ccagctaagg ctttaggaat agaagataga
     8101 gtaggctcta tagaagttgg taaagatgca gacctagtaa tttgggataa ttctccacta
     8161 gagattcaaa gtaatgtact ttacactata ataaatggca aagtagtgta tgagaaaaaa
     8221 taacttgaca attattaaaa aattaactaa tataagagtg taaggaaaat taaaaaatca
     8281 aagaagatct tcggggcagg gtgaaattcc ctaccggcgg tatagcccgc gaactactta
     8341 ggtggttgat ttggtgagat tccaaagccg acagtaaagt ctggatgaga gaagatattt
     8401 atggaagata aataagttta tttaagttta tttaagtttg tttaaactat atccataaaa
     8461 actcgaagat agggtcttcg agttttttgt ttttcctaaa aaatataaac atattgatta
     8521 gggggaaaat aattatgcaa aacacagcaa aaagaggcaa tatgatttca acaagaactt
     8581 tagtagtaat gtcaatttta tctgcaatat cttatttatt aatgtttatt caagcaccaa
     8641 taccaggtat atttccagat ttcttaaaaa ttgacttatc agatattcca gcaatattcg
     8701 gaggaatgtc tctaggacca ttagttggat ttggaatagt tgtagtaaaa aatgtgttac
     8761 aagcaataac agcaacaaca actggaggaa taggagaatt tgcgaatata ttaattggtg
     8821 gttcttatgt aatgatactt tgttattcat ataagagaag tggagattta aaaggtgtac
     8881 tgattggttt tgtattagga acaatatcta tgactataat tggttcatta gtaaattatt
     8941 ttgtaatgtt gccactttat ggtcaactta tgggactaga tgctataata ggtttaggtt
     9001 cagctataaa tcctcaagtt catgatttat tcacttttgt aatttggatg atagctccat
     9061 ttaatgtgct gaaggctgtt ataatatctt tagtaacatt gccactttat aaaaaaatgg
     9121 gaaatataat aaagaaataa ttagacaaga aaatataaaa ttaaatgtat tgaatagcca
     9181 taatttattt ataacttcag tcctatattg tgattgatat attaaatata ttatggcttt
     9241 attaatttat caaaaaatgt atataaaata agaaatattt agacaaattt aaattttaaa
     9301 ataaaggaag aattaaaacg attaactatt aaaatatgaa aaaggtatct acaatataca
     9361 gagcgaattt ataataaaca aaaaataaa
//
LOCUS       DAEMHW010000145         9323 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEMHW010000145 DAEMHW010000000
VERSION     DAEMHW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.145"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(39..1739)
                     /locus_tag="KPK67_002836"
     CDS             complement(39..1739)
                     /locus_tag="KPK67_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459774.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(2136..2699)
                     /locus_tag="KPK67_002837"
     CDS             complement(2136..2699)
                     /locus_tag="KPK67_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459775.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(2852..3565)
                     /locus_tag="KPK67_002838"
     CDS             complement(2852..3565)
                     /locus_tag="KPK67_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBG2459776.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKGFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            3857..5521
                     /locus_tag="KPK67_002839"
     CDS             3857..5521
                     /locus_tag="KPK67_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBG2459777.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPSK"
     gene            complement(5628..6527)
                     /locus_tag="KPK67_002840"
     CDS             complement(5628..6527)
                     /locus_tag="KPK67_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBG2459778.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(6554..7192)
                     /locus_tag="KPK67_002841"
     CDS             complement(6554..7192)
                     /locus_tag="KPK67_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG2459779.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(7529..8527)
                     /locus_tag="KPK67_002842"
     CDS             complement(7529..8527)
                     /locus_tag="KPK67_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBG2459780.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KKISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(8625..9200)
                     /locus_tag="KPK67_002843"
     CDS             complement(8625..9200)
                     /locus_tag="KPK67_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459781.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         3113 a         1522 c         1029 g         3659 t
ORIGIN      
        1 cactatgtta aatactatta tcaatctttt tatttaaatt atacattacc ttgattttca
       61 gaatctttgg aatctgtatt ttcatcagtt cctgtttttt cttttgcttt tttatcttca
      121 aagtttgcaa tatttaggtt atcaaattct gacatttcta ttacaccaga ttcttctttt
      181 gaatacgata ttttgacttt atcaccaacc tgagtcaatg gcaattcgct agatactttt
      241 gaagtagccc taaatattat atctttattt gaatctaaag ttacataata attagtgtta
      301 ccacctcgca catctgggct tattcttgtc actgtgcctt ctaatacctc atcttttaaa
      361 tcattatcaa gttttaaatc attaccctta gattctaatg catccttata gtttcttaat
      421 gcttcattct tatcttcacc tattccaagt acattataat cttcaacaga tacaaatgca
      481 actctcttaa caagaccagc cttatctttt aatgatgata catatgttgg ctgacccaat
      541 atattataca ttacagggaa cgtagcttca taattttttt cttgaacatt accttcagct
      601 gatttcattg cagccgtttc agtagctcct ggttgtttat ataatctagc ttcttttgtt
      661 ctagaatcaa ctaacataaa tccaattgta gattcatctc cacctgaaga tgttatacct
      721 gtataccaat aagacctgtt atcattgcca taaactaatg atgtaccttc tgttggtact
      781 aaaactccct tttctgatat aacagagttt aaaaatccat taacataaac accccagtct
      841 ttaatctggt ctgttacaaa gctttgtggt tgtattctat ccacccattt aggtgtgttt
      901 ttaacatcat aaacatttat ttttccagtt tctgcatcaa cagtggctat tcctgttgca
      961 tttgctcctc catagccaat tttatgttca tataaactta caacccaata aggtcttcct
     1021 tcatcattta tttcaagagt gaaatctgtc ataccaacat ttacaatacc atgtatgtat
     1081 aaatgtcttt gtaaatcctg atgtaagtag gcttctggtt ggtaaactat ctttattggc
     1141 tttccatcta tttcttgaac taattggacg tcttgaggat tactagctga tacttttaca
     1201 taaccacttg taccacttag agatgttatc catttaatta tgtctctgtg aacaagaggc
     1261 gctacccagt acaactctcc atctacattt tgtatatgaa attttcctaa tttggataca
     1321 ctccctattg caggaacttc tccaagcttt ttgtcaccta atttcattgc catatcttca
     1381 tctacaagac gaatgtcatt tacactgaca gggctaatgt catcagtaaa tttactttca
     1441 gttacttttc ccaatagttc tctatatgat tttgcatgaa gtacaggtgt agaagttata
     1501 aagggaacta ctacaatata cacaagcaaa gctattgcta ttgaaaaatt atatttagcc
     1561 attttagatg ctctttcatt tctatctaac atcatatcta gtattcctgc tacaacaaag
     1621 aaaattattc ctactgaaaa taaagatgta aaatctaatc ttagtacagg taatttaata
     1681 aatgcataaa ttattacaat tattaatgca actccatatg ttttaagata tcgtttcatt
     1741 taatactcct ctctaaaaaa tgttattatt actattatat ccatatattt acaaaaaata
     1801 aatatttatc atatttatta tatattaaga tatctttata tatttatagt tctgcttatt
     1861 caactatatt taacattata cgtgtatgta taaatacttt ttatacactg tttgtgctat
     1921 ccatcccgca cctaaaggag tgggattttt gcactattct gtattatata tatccaaaaa
     1981 tattataaaa atacaaatat tagcagaaat ttaaaaactg aagaaagtat tctaactaag
     2041 atttacaaat tcttaatcct atcgaagtta aactactatc ttcagcctaa ttatctagca
     2101 atattttttt atatgaaaaa ctctaaatca agtttttaaa tgtttgcgat ttttgataca
     2161 taagaatgtt gtctagaatt atattctgat aataaacctt catactctaa ggttaagcca
     2221 gataaattat aaaaatcatt tggcgttact tcaaatgctt gtttatttaa aatgtctatc
     2281 tcactttttt ctaacatgtc agcaacaaaa tttgaacata catatttata ttcagattgt
     2341 ctctgaatat tcattagtaa atatattaat gccattgtat cgtaatagta atcttctcta
     2401 taatcactaa ttaattttat atttttatat agattttcat attgtaaatt atctacttct
     2461 aacgaatata ccctacacac agtgtttttt cttatagcat ataatccttg atttatattt
     2521 tcttcaacaa atcctccaat aaatggattc cttggattta gccttccaaa agaatacatt
     2581 ggttttagat tttcatttaa tgatattgac acatgtgtat acagcttttt acttatattc
     2641 cttattaagt aagataatat agttcctgta tatgttgtta caatatagac tttattcatc
     2701 attttccccc aatctagtaa ctaaataata ttatacactt acaatttaat atcataagat
     2761 attctaacat aataaatgtg gttttacaaa ttttgatttt aaggtacttt aatattatat
     2821 aatataataa aaatataagt tttctatatt tttaaccaac agatgttttc ttcaaattaa
     2881 tcagatgatt tagtatatct tgaacctttc cagagtcctt taaatatcct aatgccccat
     2941 ttatttgtga ttttaaatct atttctactt ccatatccaa atcaacattt ccttttttta
     3001 tagatatatt tttaagtgtt attgggtaaa gataactttt ttccaaagta atcttatttc
     3061 cattaacctc aaatggaact tttttattgt agttacttaa tattttttct agcattttat
     3121 cacttagttt aaattttcca attttcacat tctcaagact tacaactaag tttccatcct
     3181 ttaaagtagg tctcaattca agttcatatt gactatttac caaaccaaat cccttatatg
     3241 gtcctgctac ctttatttta ccatttttca tttcaacaaa attattttca agttcattaa
     3301 ttccatgctt ctgcatcaat gtatatatta aatttttaaa ctcttcctct gaaacagata
     3361 tttttccaac cattctaagt ggatttttta ctacctccat agagtttata tatgagcctt
     3421 ttgataaata ttcttcatct atgattttat tattttgtga actaacatca tatctttctt
     3481 ttggtgtcaa gatatataca acaattcctg caagtactaa aacaataaca attaaactta
     3541 acaaaatctt tgcaaccttt cccataatcg tctcctttac aaatctgtca tcagatttaa
     3601 aataaattta gataactaac ttcacataat attcttaaca atatacatac tataatacag
     3661 tcttttaaaa attaattcaa ccaatctaat gttttgtaaa taaactgtaa aaacatgttt
     3721 ttagtataaa agttaatatt ttgcataata ctcttaatta cacctttatt tttttctaat
     3781 cttggattaa aattaatatt atgcaataca gtgtagtgtt ctgtattata atcaaattaa
     3841 ataggaggta tagaatatgt caaatgaaac gaactcatct aactttataa agaatattat
     3901 tataaacgac ttagaaacag gaaaacatga cagtatcata actcgttttc cacctgagcc
     3961 aaacggatat ttacatattg gtcatgcaaa aagtatatgt cttaattttg gattagctaa
     4021 ggagtttaat ggaaaagcta atttaagatt tgatgataca aacccattaa aagaggatgt
     4081 tgaatatgta gagtccataa aagaagatgt aaagtggtta ggatttgatt ggaacgagtt
     4141 aaattttgca tctaactact ttgatgaaat gtataagaga gctttaatac taataaaaaa
     4201 gggtaaagct tatgtatgtg atttgacaca agaggaaatg agagaatatc gtggtactct
     4261 aactgaacca ggaaaagaaa gtcctcatag aaacagaact attgaagaaa accttgattt
     4321 atttgaaaga atgaaaaatg gtgaatttaa agatggtgaa aaaacattaa gagccaaaat
     4381 agatatgtct tctccaaata taaaccttag agacccaatt atatatagaa tatctcattc
     4441 aacacaccat aatacaggag ataaatggtg catatatcct atgtatgctt ttgctcatcc
     4501 aattgaagat gcaattgaag gaataactca ctctatatgt actttagagt ttgaagacca
     4561 aagacctctt tatgattggt ttgttaaaga atgtgagatg gaaaatatac ctagacaaat
     4621 agaatttgct agacttaata taaataatac tgtcatgagt aaaaggaaat taaaacaact
     4681 tgtagatgag ggtattgttg atggttggga tgaccctcgt gttcctacaa tatccggaat
     4741 cagacgtaag ggatatactg cagaagcatt gcgcaatttc tgtagtgaaa taggggtatc
     4801 aaaagttaac tctacggtag atagtcaaat gcttgattat ttcctaagag aaaacttaca
     4861 accaaaggct cctcttacta tgggagtttt aagaccttta aaacttatta taactaatta
     4921 cccagaagat aagattgaga tgcttgaaat agagaataat gctaaagatg aatctcaagg
     4981 aaagagatta gttcctttct ctagagaatt gtatatagaa caagatgatt ttatggaaga
     5041 acctgttaag aaatatttta gatttttccc aggaaatgaa gtccgtttaa aaggtgctta
     5101 ttttgtaaaa tgtactgatg taattaagga tgaaaatgga aatgtagttg aaatacatgg
     5161 tacttatgac cctgaaacta agagtggctc tggctttaca ggacgtaagg taaaatctac
     5221 aatacattgg gttgatgcta aatcagctat accatgtgaa tttagattat ttgaaccatt
     5281 aattcttgat gatatacctg aaaatgaagg taaacatttc ttagaacaaa taaatcctaa
     5341 ttcattggaa atattacaag gttttgttga acctacacag ataaaagatg ctaaaccttt
     5401 tgataaattc cagtttgtta gaaatggatt ctttagtata gataataaat atacaactga
     5461 tgaaaaattt gtatttaata gaatagttcc tctaaaaagt tcttttaaac caagtaaata
     5521 atcttacaat caaaaaatga agacttaaaa tataaattac ttctactata aataaactta
     5581 gttagaagta attttttatt tgagtcttca tcatttactt taaatgatta acttttttca
     5641 ctacagattc ccatgacttc tattccatct tttatgcatt ctatgttaag aggaaaatct
     5701 ggtccttttt ctccgtcaat atctgttata agtgaatctt cacattctat atgcaccttt
     5761 ttagttttaa agtatagtat ttcatttcca ttatattgat ctaaataatc acctctaagc
     5821 acacttatca aaactggtat tgattttggt attggcattc ctttaaaaat aattacatcc
     5881 aaatatccat catctatttc agccttatat gctagattta tatttcctgc tgttttgcca
     5941 ttaaaaatca acataagata catatctcca tcataataca tttcctcaga agatactttt
     6001 atcttaaatt tcctcatata caatgcttct tctattccct ttatataata agaaattctt
     6061 ccaaatgaat tttttaagtc tgtgtttatc ctttgtgata catccgtaaa cataccagca
     6121 cttgcaacat ttataaagta cttattattg actttcccta tgtctatttt ctttggtttt
     6181 gaatttatga tttcatttat agcttctttt atatcaaatg aaatttgaag agcatttgaa
     6241 aaatcatttg cagttcctgt aggcaaaatt ccaattggaa tttttatgtc taattctttc
     6301 attgcattta ctactaaatc tacagtacca tcaccaccag ctatcaatat gtgttcataa
     6361 gaactatcaa cttccttaaa tgcattcttt acaggttctt taatagttaa tctatagggt
     6421 atcaataaat aatttttttc ttgatataat tctatgatag tgtctaaatt tgataatatc
     6481 tttttttcac ctgaattagg attgtatata agttttactt ttttcattat ttctccttac
     6541 tcattttatt cttttagtat ccaaaatctt catttaaaaa aatcacatgt attctttctg
     6601 gtgttaattg tctttttata agtgtataac tacagcatat tcttcctgga cttgaacagt
     6661 ctatacatat accagtagtc ttacatccag tatttgtact taatctttta caattggctg
     6721 gagcagctat tgtttttgtt ctttttatag cttcatctac atctttaact attttattta
     6781 ctcctgctat aactattact ttatcaggtc catatatcat agcagcaact ctattcccat
     6841 tcccatctaa attgtaaagt tctccatcct ctgttatcgc attagtactt gtaaaataag
     6901 catctgcaga aaatgacttt ctatatattt ctttcatttc ttctggtttg atatcttttg
     6961 catatctatc aagaaaatta tatttttctc ttataaaatc aatcactcct gtttcaaata
     7021 aggtcataga acctccacat cctactagag aacctgcttt aactatttct tcaatttttt
     7081 taagaagctc ttctttgtta cttactaaat accctttcat attattgttt tccaaagatt
     7141 ttattgtttt ttctattctt ttttcattaa gccaatttaa attttgatcc attttaactt
     7201 cccccttatt tttaatatgt acttaattat ttataaactt tattttcttt ataagtttat
     7261 tttagtacat ctaaaaaaaa atacaatata aaacttaaat agttttacat aattttttcc
     7321 aaaataactt actcaatcaa aaaaggaaag tagatgtact attctacttt ccctatattg
     7381 tatgtagtat ttattgccta aaatctatat attaattata tgtttcatat tatataatat
     7441 cacagttaat taaaatgtaa tactatcttc tttttaaaca tataaattgt attattaaat
     7501 ttaatatatc caacttcaat aaatcttatt aatttgatga agaagatgct atagctgcac
     7561 ttgttgctgc cattacacat gcaatttgaa tagcaacaac aatatttaaa aatatattat
     7621 tagttataga acctatatta ttctcatttc gtatcaaatc tgcataaatt gaagctacaa
     7681 tagcagaact aatcattact ctattacttt ttcctaaaga ccaatttcca aagccttttt
     7741 cttctttaag tttatttgaa acatttttta tctcttctat tatttcatct tctctataat
     7801 ctaataatga aattgctcct attattggat atccataata attcatttta caatcatttt
     7861 ctttaaatgc ttttttaagt tcaatagtct ttttacattt aagcatagtt ctatcatcac
     7921 taaataaaag aatttgggat aatgcttgta aatcatttcc tttataaaaa ccattttcat
     7981 ttagaaattc ataacatttt tctatgtatt ctaaatcttc atctaaattt tttgaatgaa
     8041 ttgcaataag tgctatatta caatagtcat cactagaagt tagaaatgga tgttttttcc
     8101 tcatgtaatc atatattatt ttcatttttg atatacatgt aagataatcc attttctctc
     8161 tattttcata aactattgat gctgtaagtg gtaaatatgt atcgccccaa aacttttcac
     8221 ttttaagttc tttgtatata tctaaaatca tcttaaaact actttctgga tttgattcaa
     8281 atgaaagtag tgttgccata taaaacatat tatatcctct aaaattagag aataatccag
     8341 tatttttttt aataagctct attcgttctt ccactaatct aggacttatt ttcttgttct
     8401 taagggtaaa tgcaagtgca caactatgtt gtatcaatcc catttgccaa gaacctttaa
     8461 cttcatttaa tgctttgtaa ttttctatta ataagtttac ttttccattt ataaacgatt
     8521 gttccatatt gccctcctag taatatatct cattgtacta atagttttat atttattata
     8581 tatatattaa atactacaat caatacatat tacttcaaag aacatcattc ttcaacaata
     8641 atgtagcctt ttttgtaatg tcttcccata ttattgactc tatcatagta ctctgattca
     8701 ctttcattac tccatttggg cacaaataca tctattgcat tagaattact aaaatactta
     8761 ctagaccccc tatccataac agtttttaat tttccatcta caataatctt agttccaaaa
     8821 cttaagtgat tgtttgctac aaaaccatct tctaaatcat ctcctgtagc agttatcgct
     8881 ccatatccat tttcttgatt aagacttgtg taataactaa cttcaaaagt tataggtact
     8941 aacttcttta ttttaatacc taactgattt tgtagtttct ccttttgtac tttcaactta
     9001 tactcatcac tatttttata atcttccata ccttgattat acttggaatt aaagctttta
     9061 cctgacttta ctatagtatc tatatttact atagattctt tacaatgatc tgcatctatt
     9121 tttgtttctc catttaccaa taatggagtt aaacatacac tacatacaac acatgtaatc
     9181 aatgcttctt taagcttcat aaattccccc cttattattt tgatatatta gcatatttaa
     9241 tattatatgt ctacatttag atttaaatac cacacccatt tgctttaaga agttgtctta
     9301 tatttaattc accttaaaca ctt
//
LOCUS       DAEMHW010000146         9240 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEMHW010000146 DAEMHW010000000
VERSION     DAEMHW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.146"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            552..1460
                     /gene="trxB"
                     /locus_tag="KPK67_002844"
     CDS             552..1460
                     /gene="trxB"
                     /locus_tag="KPK67_002844"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG2459782.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDREVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1550..3373)
                     /gene="typA"
                     /locus_tag="KPK67_002845"
     CDS             complement(1550..3373)
                     /gene="typA"
                     /locus_tag="KPK67_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBG2459783.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVINCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3585..3827
                     /locus_tag="KPK67_002846"
     CDS             3585..3827
                     /locus_tag="KPK67_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459784.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3975..4166
                     /locus_tag="KPK67_002847"
     CDS             3975..4166
                     /locus_tag="KPK67_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBG2459785.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            4450..6951
                     /locus_tag="KPK67_002848"
     CDS             4450..6951
                     /locus_tag="KPK67_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBG2459786.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            7111..7779
                     /locus_tag="KPK67_002849"
     CDS             7111..7779
                     /locus_tag="KPK67_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2459787.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRIEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            7763..9040
                     /locus_tag="KPK67_002850"
     CDS             7763..9040
                     /locus_tag="KPK67_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG2459788.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
BASE COUNT         3646 a         1075 c         1563 g         2956 t
ORIGIN      
        1 attacaaaaa aagtttacaa aactttaatt tgaaaaatgt aattatatgg tatatcatat
       61 aaatataagt aaatagtgct aatatttaaa ctattaatca taattggttc aacaaaagta
      121 tatagttaat aatgtttaat ttgaagaaat tgttagtgtg aaattacata tatattcaaa
      181 ttaaattcat aaatatttca cataataata gtatactgta aacaaaagga ggtattaaat
      241 atgggaatag tacttataac agcagcatta gttttggtta tgtatggact ttcaattcta
      301 tcatatatat cagaaagcaa tatattagaa gacactgtaa atgataacca ataaggtttt
      361 gaattatata aataagtgag aactgtaaat aaaagttaat ttttatttgc agttttttta
      421 tatatatgta aaataaagtt aattaataaa aaataatgtt ttgtttatag tatatatgga
      481 taataatagt aaagtataag aatgatgaga ataatatata agaaataata atataaagag
      541 aggtaaacgt tatggtagat atcattgtaa ttggtgctgg accagcaggt ttaacttcag
      601 caatttatgc tatgagagca gggttaagcg ttacagtatt tgaaaaaaat atttatggag
      661 gtcaagttgc aagtacctct gaggtagaaa attatccagc agttcaaaag atttctgggg
      721 tagaattttc taataatata tacaatcaag cagttgctca aggtgttgat attcagtttg
      781 atgaggttga agagataaat ttagagggaa aagtaaaggt tgtaaaaact tcttcaggtg
      841 aacataaagc aaaggctgtt attcttgcaa atggagttga aagacgtaaa ttaggttgtg
      901 caggggaaca agaatttaca ggtagaggag tatcttactg tgcaacatgt gatggagcct
      961 tttttaaaga tagagaagtt gctatagtag gtggaggaaa tactgcacta gaagatgctt
     1021 tatttttagc taataattgt acaaaagtgt atttaattca cagaagagat agttttagag
     1081 gtgaagaagt gttggagaaa tctgtgaaag caagagagaa tattgagata ctttacagtc
     1141 atggtgtaga aaaaatagaa ggagaaaaga ctgtatcgaa aatagaagta aaaaacttaa
     1201 agactgaaga aaaaagaact atagatgtta gtggtatttt tattgctata ggtcttaaac
     1261 ctaataataa aatgtttgaa aatgttcttg atttggatga aggtggatat ataatttcag
     1321 atgagtcttg tacaacatca gtagaaggtg tatatgtagc aggagatagt agaactaagt
     1381 ttttacgtca aataataaca gcagcatcag atggagctat tgcagccgtt caagcagcta
     1441 attatataaa tgttgaataa tataaatttc aaataaaaat agccatataa actgaaataa
     1501 tttttcagta ctatatggct attttgttaa ttagaggtaa atgtacgttt tatttacctt
     1561 gtctagattt gttatatctt aatctatcat gttcatttaa gaatcttttt ctaagtctta
     1621 tagaatcagg tgtaatttca actaattcat catcttctat aaattctagt gcttcttcaa
     1681 gagtaaatat tctaggagga gataatttta tagcatcatc tgaaccagaa gcacgtacgt
     1741 tactcatttt cttgttttta caaggattga ctaccatatc atcttttctg gaattcatac
     1801 caattatcat accttcgtaa acttctacac cagggtctac aaacattaca gctctatcac
     1861 ttaaggcatt aagagaatac cccatagtaa caccaggtcc ttgtgctatt aaaacaccat
     1921 ttcctcttga aggtatttct cctttaaatt cttcaaattt ttcaaatgaa cgaactaaag
     1981 taccttctcc acgagtagcg tttataaatt cacttcgata tcctaaaaga ccacgagtag
     2041 gtgctaagaa ctctattttt acatagtctc cttctattga catagactcc atcatacctt
     2101 ttctcatgtt taattcattt ataatagtac cagagtaaac ttcaggacag tttataacaa
     2161 ctctttctat tggttccatt agttttccat cttctttatg cattaaaact tcaggtttag
     2221 aaacaccaac ttcataacct tcacgtctca tattttcaag tagtatagat aagtgaagct
     2281 ctccacgtcc agaaactttg tatccatcag tagtatcaag aggttcaact ttaagaccaa
     2341 catttacttc aagttccttt tcaagtctat ctttcaaatg tctagttgta acgaattttc
     2401 cactttttcc aacgaatgga gagtcgttta ctaagaagtt catagaaaga gtaggctctt
     2461 ctatttttat catttccata ggtagtggac tatctaaatc gcatatagtt tcacctatag
     2521 atatatcagg aataccagct ataacaacta tttctccact agtagcttca tcaacttcaa
     2581 cttgttttaa accttcatac acagaaagtt ttgatacttt tcctttagat acaacagaat
     2641 cttctctaca tatagcaact tgtgtattgt ttttaagagt acctttgtaa attctaccta
     2701 taccaagtct acctacataa tcatcatatg caagagctga tatttggaat tgtagaggtt
     2761 cattatcgta attaggataa gcttcaacgt gatttacaat agttttaaat agaggagaaa
     2821 ggtcttcact atcatcatcc atttcaagtt tagcaatacc ttgtttagca ataccatata
     2881 ttataggaaa ttcgcattgc tcatctgttg catttaagtc tacaaataaa tcgaaaactt
     2941 cattaactac ttcttccgct ctctggtctt ttttatcaat cttgtttata aataatattg
     3001 gttttaaacc gaactctaaa gatttttgta aaacgaatct agtttgaggc attgggcctt
     3061 cactagcatc aactaataga ataactgtgt caacagtttt cataacacgt tcaacttcag
     3121 aagaaaagtc actgtgtcct ggagtatcaa ctatgtttat tttgtaatct tcatagttta
     3181 ttgcacagtt ttttgaatat atagttatgc ctctttcttt ttcaaggtcg ttactgtcca
     3241 taacgcagtc tttaactact tcgttctttc taaaaacacc gctttgagaa agaaaagcat
     3301 caaccaatgt tgattttcca gcgtcaacgt gggctataac tgcgatattt ataattttat
     3361 gtttttgtga catttttgtt gctcccttcg attaaaataa cttattaatt atagcacaat
     3421 atattattag gtacaaggag gaatatatta aattgtatta atatagttta aattggataa
     3481 aactgttagt tttgcatctg aaaattattc tgtttaaaaa aattttttag acaaataata
     3541 accttaatag ataaaaagat taataaaaga gtaaaggatg atatatgttg gaagtaacca
     3601 aaataataat taaatatttt gcaagtgaaa gtgatacttt tgtaaaagaa gaagtttgta
     3661 attttgaaga acttcttaag tggtctcata aatttattga aggtaaaaag atagaagatg
     3721 ttttgtatat aaagtctaaa gaaaactata aagtaaatgt gattaagtct ttagaaatta
     3781 aggaattgga aatagtgaga aaacaacaga taccaccgta catgtagtat taataatatt
     3841 taaaattaat atgtaatact cacaatagga tataaatggt atatatggta ataatttata
     3901 ttaaaaaaac ttttataaag tttatatttg ggtataaaag tatcaattag acattaatta
     3961 ggaggaactt aaatatgata agagtatgcc caaactgttc aaatgtggat gtaaataagc
     4021 taaaaacttt ggttggagaa gaaaatgtaa agactggttg tataggtcag tgtagagcat
     4081 ttaaaaaaga agctgttgga ataatagaca atgagcttga aataaaggaa acagaagcag
     4141 agttttttga agcatgtaaa gaataaattt taaataaatt ataataaata gagcatctaa
     4201 attgtataag cattaaacaa tttagatgct ctatttttat atatacattt aattatgagt
     4261 atacttttct attaaataca aataaatata actaatacat ttatttatta aaaaaatatt
     4321 gatttaatat acctataggg ggtataataa aaatatagat ttctaaataa gtaatttatc
     4381 aatattacat atcaaaataa aacgttacaa attagctgaa aatatattga aagaaaggaa
     4441 gattttatta tgagttcaaa taaagtggta gaaagttata aaattacagg tatgacatgt
     4501 gcagcttgtg caaaggcagt agagagagta actaagaaga tggatggagt atatgaccaa
     4561 agtgtaaata tagctactga aaaacttaaa atagaatatg ataattctaa ggttaatttt
     4621 gatgatataa agcaggttgt agaaaaagca ggatatggaa ttataaagga agaaagtaat
     4681 aaaaaaattg atatgaaaat tgatggaatg acatgtgcag cttgtgcaaa agctgtagag
     4741 agagtggtaa aaaaattgga tggagtagaa agtattagtg taaatattgc aacagataaa
     4801 gctaatatag actatgaccc atcaaaagta aaattatctc aaataaaagc agctattgaa
     4861 aaagctggat ataaaccaat agaagaggta agaaataagg ttgatgtgga tgaagataaa
     4921 ttgagaaaag aaagagaaat gaagtcttta tttgttaaat ttatagttgc aatagtattt
     4981 gcagtacctc tgttttatat agcaatggga cctatgataa taaagcctat agggccatgg
     5041 cctttgccag aaattataaa tcctatgaca aatactttta attatgcatt aattcagtta
     5101 attttagtaa taccagtaat gatagctggt tataaatttt atataaatgg gtttaaatca
     5161 cttttttctt taagccctaa tatggattct ttagttgcaa taggaacatt agccgcattt
     5221 ttatatagtc tatatacgac actacaaata gccaatggac aaattcaagg tatgcaccat
     5281 catcagttgt attatgaaag tgcaggaata ataatagcac ttatcttact tggtaaatat
     5341 ttagaatcaa aatcaaaagg aaaaacatca gaagcaatta aaaagcttat ggggctacaa
     5401 cctaaaactg caatagtgtt agttgatggt aaagaagttg aaactcctat agaagaagta
     5461 gagataggag atatactatt agtaaaacca ggaactaaaa taccagtgga tggagttgta
     5521 atagaaggat atacatcagt tgatgaatca atgcttacag gtgaaagtat accagtagaa
     5581 aagaatgttg gaagtaaggt gacaggagct agtattaata aaaatggtgt tattaagttt
     5641 aaggctgaaa aaattggtgg agatactgct cttgcccaaa taataaagtt agttgaagat
     5701 gcacaaggta caaaagcacc tattgctaaa cttgccgata ctgtatctgg atattttgta
     5761 ccaatagtaa ttgcaatagc tgtagttgca tcattattgt ggtttttaat aggaggaaaa
     5821 gatatagtat ttgtacttac tatatttata tcagtacttg taattgcttg tccttgtgca
     5881 ttaggattag caacaccaac tgctataatg gttgggactg gaaagggcgc agaaaatgga
     5941 atcttgataa aaggtggaga ggcattagaa tctgcacata aagtaaatac agtgatattt
     6001 gataaaacag gtactataac agaaggaaag ccaaaagtaa cagatatagt attaaataat
     6061 aatgtaaaag aagaatatct tatcaagatt gcttcaagtg cagaaaaagg ttcagaacat
     6121 cctcttggag aagcaatagt taagtatgga gaagagaaga atataaaatt tgaaaaagtt
     6181 gataatttta aagctatacc aggtgctggt atacaagtta ctattaatga tgaaagtata
     6241 ttacttggaa atagaaagct tatgaatgac aataatataa agcttgggga tttagaagaa
     6301 aaatcaaata tattggcaag ccaaggtaaa acacctatgt atatagctgt agatggaaat
     6361 ttatcaggta taatagcagt tgcagatgta gtcaaagaaa gtagtaaaaa agcaatagaa
     6421 atacttcatg atatgggaat aaaagttgca atggtaactg gagataatgc gaaaactgca
     6481 aatgctatag caaatcaagt cggtatagat atggttttgg cagaggtttt accagaagat
     6541 aagtcaaaag aagttgaaaa actacaaaat caaggaaagt ttgttgcaat ggtaggggat
     6601 ggaataaatg atgccccagc tcttgcaaaa gcagatatag ggatagcaat aggaagtgga
     6661 acggatgtag ccattgaatc tgcggatata gtacttatga agagtgattt aattgatgtt
     6721 ccaacagcaa ttaaacttag tcatgaaact ataaaaaata taaaacaaaa cctattttgg
     6781 gcatttggat ataatacaat tggtatacct gtagcagcag gaatattata tgtatttgga
     6841 ggacctttat taaacccaat gattgcagca gcagcaatga gcttaagttc agtttcagtc
     6901 gtatcaaatg cacttagact taaaaacttc aaggcatata agagagatta gattaataaa
     6961 ttaaataaaa tgtatccaaa agagctatct ttaaattaaa aggattaatt tacaaattaa
     7021 gatagctctt ttttcatgta aaattcacaa caataagata aaatataagt aatatacgaa
     7081 attttaaata aaatagaaag gattgataga atgaattcgt caatacttgt aattgaagat
     7141 gattcaaata tacaagagtt aatatcagaa tttttaagtg cagaaggtta tcaagttgat
     7201 acagcaaatg atgggttgga aggtatacaa aagtttaagc aaggaagtta tgatttagta
     7261 atattagata taatgatgcc aaacttagat ggttatggag tttgtaaaat gataagaaag
     7321 tcatcgagcg ttccaattat atttttaact gctttaaatg atgaaggaga ccaacttaaa
     7381 gggtttgact tagaatgtga tgattatata acaaaaccat tttcatttaa tcttcttata
     7441 aaaagaatag aagcaatttt aaggcgaagt aacaagacta taaatgacaa atttatagtt
     7501 tttgaaaaat taaaattaga tttaaacaca tatattgcag agatagatgg agaacctata
     7561 gaacttacac taaaagagtt taatatatta aaagctttga tagaaaagta tccacaagtt
     7621 ataactaggg aaggtctttt ggatagtata tggggttatg actattatgg agatactagg
     7681 attgttgatg cacatataaa aaatataaga aaaaaaatat cattaccata tattaaaact
     7741 gtaaaaggaa taggatatac acttgagaag gatatttgat aagtgggaaa agttaagtat
     7801 aaagtataag ttattttcaa taacgacatc attattaata gcattagctt taataatata
     7861 tctaatttta tattttttat tgccatcata ttatcatgag tataaaattg agtctcttca
     7921 agaaagtttg aaatctttag tagatagttc aattcatttc gatacatata ctttagaaga
     7981 aagactttat tatatggcta aagaccaaaa tttggctatt ttattaaaag ataaccaagg
     8041 taaaattgta tatggtaaga atgaagtagt tattttaaga tatagtaagt acatgattaa
     8101 tagtcttgag gatgagtata gaacttctat acctatatat actaaagatg caaaagatgg
     8161 tccatatact ttagaactcg ttatgccatt acaaccaatt gacgaagcaa acgaagttat
     8221 aagaaaacta atgccataca tcatatcaat agcaatattg atagcgatta ttggggcata
     8281 tatatattca attgtaataa caaagcctct tataaacata atagagagtg agagagaaca
     8341 ggaatataga aggaaggatt ttgttgctac aatatcacat gaactaaaaa ctccaataac
     8401 cataataagt ggtcaaattg aaggtatgat ttatagtgta ggtaagtata aggataggga
     8461 tacttatctg aaaaaatcgt atgagtgtac acaagaatta aaagatttag taaatgaaat
     8521 gatagaggtt tcaaaatcag aaatacttga aaaagatttg aaattggtat caataaatat
     8581 aagtgaatta ttaaatagac ttgtaaagag acaagtattt ttaatagaag aaaaacatat
     8641 gaaaactatt ttaaaaatag aagaaaattt ggaaattaag gcagaccaag agagaataac
     8701 taaggcaatc aataatataa taaataatgc cataaaatat tctcctgaag aatcagagct
     8761 tataattaga ctctatgata aaaataaacg aataagtaaa aagacttcta atcaaagagt
     8821 ggttttagaa attgaaaata ctggtgtaac tatcgagaaa cgttatttag aagaaatatt
     8881 taatccattt tatagaatag aaaaatctcg ttcaagaaaa actggaggaa gtggacttgg
     8941 tctttatata gtcagccaaa tttttaaaag tcatgggttt gattatagca taaaaaacaa
     9001 agaaaactct gtagtattca cagtagaatt taaaaattaa ttacttatta taaagaatat
     9061 ataactttca ataatagttc attttaaaat cacaaaaaat tcataatgaa actgtattat
     9121 ataaatataa agaagcttaa aaccataaaa tacaccaact ataatgatga gatttcctta
     9181 catcctccat ataagtcatc attatagtaa cccaacaatt aagttactat gaatttaaaa
//
LOCUS       DAEMHW010000147         9207 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEMHW010000147 DAEMHW010000000
VERSION     DAEMHW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9207
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.147"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            80..1066
                     /locus_tag="KPK67_002851"
     CDS             80..1066
                     /locus_tag="KPK67_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG2459789.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     IRPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1235..1383
                     /locus_tag="KPK67_002852"
                     /pseudo
     CDS             1235..1383
                     /locus_tag="KPK67_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1402..2883
                     /locus_tag="KPK67_002853"
     CDS             1402..2883
                     /locus_tag="KPK67_002853"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBG2459790.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENNENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            2909..3880
                     /locus_tag="KPK67_002854"
     CDS             2909..3880
                     /locus_tag="KPK67_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBG2459791.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4136..4738)
                     /locus_tag="KPK67_002855"
     CDS             complement(4136..4738)
                     /locus_tag="KPK67_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2459792.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKIIMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSHLKYIGM
                     LVERDLGKPNFMNKEKEEDSRQFAKQIYYILDNSIEIDNC"
     gene            5019..6230
                     /locus_tag="KPK67_002856"
     CDS             5019..6230
                     /locus_tag="KPK67_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBG2459793.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKYNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYELPK
                     QYYKRKDGYIE"
     gene            6298..6495
                     /locus_tag="KPK67_002857"
     CDS             6298..6495
                     /locus_tag="KPK67_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBG2459794.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            6529..7500
                     /locus_tag="KPK67_002858"
     CDS             6529..7500
                     /locus_tag="KPK67_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2459795.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTIFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7687..8064
                     /locus_tag="KPK67_002859"
     CDS             7687..8064
                     /locus_tag="KPK67_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2459796.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
     gene            8595..>9207
                     /locus_tag="KPK67_002860"
     CDS             8595..>9207
                     /locus_tag="KPK67_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG2459797.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKLMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISIF"
BASE COUNT         3696 a          946 c         1438 g         3127 t
ORIGIN      
        1 actggacaaa taactatatt ttattttaaa tatataatat aatgtaataa aatatatcaa
       61 atgtagaagg tgataaccta tgaaaaaaat aacaataaat gatattgcta acttggcagg
      121 agtatctaaa agtactgtat caagatatct aaacaataaa gatatcagtg attctactaa
      181 agagaaaata aagacaataa tagatgaata tggatatgag ccgaatgcat ttgctcaaag
      241 tttgagagct aaaaaaacat actttattgg tattataaca ccgtgtttag attcttttgt
      301 aaaatctaaa attatgatgg caatagatga agaactcaaa gaattaaaat atacatcact
      361 aattattaat actaatagaa aaataaggtc agaaatagat agtatatcaa agttagctag
      421 tctaaaagtt gatggaataa tactaattgg taccgaaata actaaagaac ataagaatga
      481 aattgaaaag ttagatatac cgatagttgt agttggacaa aaagttgatg gtatcaatag
      541 catagtgaat gatgattatg gagcaggata taaaatgggt caatatattg ctaataaagg
      601 ttacaaaaac attgtatatt taggtgttga tgaaagtgat atatcagttg gtttaaatag
      661 aaaaaatgga gtacttaatg ggttaaaaga taaagggtat gatgcaaaag tattttatac
      721 tgactttgac caagaaacgt ctatacaaag aagtggagaa atgctagaaa gtgaaaatcc
      781 agatataata atatgtgcaa ctgataatat agcaatagca acaatgaaag aaatcaataa
      841 gagaggtaaa agtataccac aagatatatc tgtagctgga tttgggggat atgatgtatt
      901 gtctattata agacctaaac ttacaacaat aaagtttgaa aataaaaatg caggtaaagt
      961 agcagcaaat acgatagtaa acttaataca agaaagaaaa gaaccactat taaaggagat
     1021 taaatttgaa ttaatagaag gtgaaagtac aataaataaa aattaagata cacattacat
     1081 atattagtaa tgtgtatttt tttactaaaa aaactataat tctaaaaatt aggaaaactt
     1141 ttttgaaaga aatattgcat ctgaaataaa tttatgctaa tctttaatta aaccggtttc
     1201 gaaaccggtt taattaaacc gatagaatag caaaatgagt gcaaaaataa ctgattgtaa
     1261 caaagtatca aagtaattat tagagtacat aggaataaac aaaatataaa agatgtagca
     1321 cattgtgtaa taagactaag aattgtttta gatagcaata agaaagcaaa tataaaatta
     1381 taaaagaaaa ggaataaaaa tatgtacaaa gagcctaaat atagaaccat attagaatcg
     1441 acaaaagaag aattaaataa attaagaaat atatctttaa atgataaata taaaccacta
     1501 tttcatatac atccacaaca tggactactt aatgacccaa atggattagc atattataat
     1561 ggtaagtatc atgtatttta tcaatggtat ccatatgatg caactcatgg aatgaagcat
     1621 tgggcttatg taagttctga tgattttgta aactggaata gagaagatgt agcattaata
     1681 ccaatcgaaa gttatgaatc tcatggagca tattcaggta atgcgataga ggtagatgga
     1741 aaattgcata tgtattatac agggaatata aaatatagtg cagaagatag atatgcatat
     1801 caaaacttag ctataatgaa caaggatggt aaaataacta aatatgaaaa taatccaata
     1861 gtaagtgaaa taccaaaagg atatacagga catgtaagag acccaaaagt atttaaaaga
     1921 aaggataaat actttatgtt acttggagca caaacaagtg ataaaaaagg agttataata
     1981 gtatatgaat ctaaaaactc tatagattgg aattttaaag gagaattaaa tgtaaaaaac
     2041 atagatgaag attttggtta catgtgggaa tgtccagatt atattaatat agatgaaaaa
     2101 gatatattaa tattttcacc acaaggagta gaacctaaag gatttgatta tcaaaatata
     2161 tataatgtag tatatgctat tggtaatatg gatttagata atttaacatt tgaaatagat
     2221 acaatgaaag aactggaaaa aggatttgat ttctatgctc cacaaacatt tataaaggac
     2281 tcacaaataa tactatttgc atgggctgga atgggagaag ttttataccc tacagataaa
     2341 aacaaatggg cacattgtct aactgttcca agaaaattaa atataaaaaa taataagcta
     2401 ttgcaaatgc ctgtagatga attaattaaa ttaagatatg atgaaacaag tgggcaaaat
     2461 actataaaga ataatatcaa tattatagaa aataatgaaa atgtatatga gcttaatatt
     2521 aatataaaaa atattgacag caataaattt gggctggaat tattttcatc acaagatgaa
     2581 ggtgtaaaat tagaatttaa caaattagga aacattgtaa ctttagacag aagcaacttt
     2641 aaaaaagttt tcggtgttga atatggaaca aatagaaaag aatatataaa tattgatgaa
     2701 aatactaata tcaaagtact agcggataga agtattttag aaatttttat aaatgatgga
     2761 gaagtagtat ttactagtag aatatttgct aaagaaaatt ctaatcaaat aagggtttat
     2821 agtgataaaa tagtagagta tgagtatact aaatttaaat taaagcaagg tattgaatta
     2881 taaaaacaca ttataagggg tagaaaagat gaaaaaagta attagcatag gagaagcttt
     2941 aatagacttt ataccgaatc aaaatggtgg taaattaaaa aatgtatcag aatttagaag
     3001 agtagcagga ggagctccag caaatgttag tgctgttgta gcaaagcttg gaggaaaatc
     3061 aagttttata tctaagttag gaaaagatgc atttggtgat tatataatag atgtattaaa
     3121 tgaagtaaat gtaaatactg attatgtatt gagaacaagt aaagcaaata caggacttgc
     3181 ttttgtatca ttaaaagaag atggtaatcg tgatttctca ttttatagaa atccaagtgc
     3241 agatatgctt ttagaagctg atgaagttaa aaaagagtgg tttaataatt gtcatatcct
     3301 acatttttgt tcagtagatt taatagatag tccaatgaaa ttagcacata aaaaagcaat
     3361 agaatacgca cttgaaagta atagtataat aagctttgac cctaatataa gattaccttt
     3421 atgggatagt gagcaatctt gtaaaaaggc aatatcagaa tttttacctt ttgctcatat
     3481 tgttaagata tctgatgaag aattagaatt tataacagga gaaaatgata tagaaaaatc
     3541 attacataaa ctctttgtgg gtaatgttga actagtatta tatacaaaag gaaaagatgg
     3601 agtagatgca tacactaaaa aagtaaaagg tatgtgtaaa ggggttaaag taaatgcaat
     3661 agatacaacc ggagcaggag attcttatat aggttcattt ttatacacct tattgtataa
     3721 acaaataaca ttgaatgata taaaagaaat gaaacaagaa acgttaaatg agtatttaga
     3781 attttctaat tattatgcag ctcgtagtac aactattaaa ggtgcgataa gttcatatgc
     3841 aacaaaggaa gaaataactg aatttataaa aaatttatag taggaatgag taataggttt
     3901 tgaaggaagt aacatgtaaa gagtttttaa ggatgtttac tatgaaaata attaacatat
     3961 aaaaagcaat aaagaggctg tctcaaaata gagataagcc tctttatcat tgttgaaatg
     4021 ataaaaaata ttttaattat tgttactttt tagaagaata tatatcttat tttaaataag
     4081 caaattatgt aagatttatt tatattatca atactttact tggagtatta tgtctttagc
     4141 agttatctat ttcaattgaa ttatctaaaa tataataaat ttgttttgca aattgacgag
     4201 aatcttcttc tttttcttta ttcataaaat taggtttacc taaatcacgt tcaacaagca
     4261 ttcctatata ttttaaatgt gaatgcttac agtagcgctt cataccattt tcccaaatat
     4321 cagcaccctt ttcaggtcta tatccacatg ttgtaataag tgctactggt tttcctgccc
     4381 atagagcggg gcctttctca ttaccatagt atttattcat tccatacacc aatctgtcaa
     4441 gtagtgcttt cattggagaa gtgcagtacc aagaataaat tggagtagca aaaacaatta
     4501 aatcacacat aataattttc ttgaaaattt tctgaacatc atctttacaa taacatccaa
     4561 aatcaaataa gttatcctga cactttttac aagcaataca tggctctatt ttacaatcat
     4621 ataaccatat tacttcaata tttgaattat aattttctaa ttcctcaata aaaggtttta
     4681 aaatagcagc agtattacct tttttcttag gacttcccat aagaatgcat acttccatat
     4741 tgatacctcc acagtatttt aaaatagttt ttagtgctta atatagtttt aggatactat
     4801 gagaatacgt taacaacaat acactttgta tatttgtaat aaaattgaaa caaattgttt
     4861 tatcaataaa agttggaatg tatctataat taaaaatatt ttaggtatgt ttttacatat
     4921 atgtgtgtag ttaactatgg taatttattg taaaattatc ttataggatt ataattgctt
     4981 aatttatgat tatatttaaa atcttggagg taaaaaatat gtactcaaat gaagactata
     5041 aatttataga agaaaatgca ccatgttata tatatgatga gactaatata attgaaaaat
     5101 gtaaggaatt gaaaaataat attttaggtt ttgatttttt atattctata aaaacaaatc
     5161 catataacaa aattgtaaaa agcattgcag agcaaggttt tggagcagat gcttcttcaa
     5221 ctaatgaagt tatgatttca tcagaatgtg gagtttctaa agatgatatt tattattcat
     5281 caccaggcaa aacagagatt gatatagaaa ctgttttaga taaatgcata attattgcag
     5341 atagtattac agaaatagaa cttataaata agatatctaa aaagtataat cagaaagtta
     5401 aaataggaat aagaataaat cctgatttta ctatggatga tgatagtgct aaatcaagca
     5461 aatttggtat agatatagaa gatatgagca gtgtttttaa gatgttaagt atgtgtgatt
     5521 atgtatctgt agttgggatt catatacata ttaaatctca ggtattggat tttgaaaagt
     5581 taggacatta ttatgaaaaa tgttttttta tagctttaaa tttaaataaa agtaagtata
     5641 ttaatattga atttataaat tttggtagtg gacttggagt agcttatgat aatcttagag
     5701 aaaatccact taatcttaaa gagctttcaa aattaactca aaagattgct tttactaatt
     5761 caaacacact aaaatcaaga cttcttattg aatcaggacg tttcatagtt tgtaaatcag
     5821 gtaattatta tgttaaaatt gtagatatta aagtatcaag agggaaaaaa tatcttattg
     5881 taaaaaatgg acttaatgga tttatgagac ctattatttc taatttaatt gaaaatatta
     5941 cttgtggagt agaattagaa ggtcaagagc cattatatac atcaaatcat gccttttctc
     6001 tttcagtatt aaataattta aaagaagaag agtatgtaaa tgttgttggg aatctctgta
     6061 catcattaga tgtaattgca aataatgtta aattgaaaaa agcaaacata ggagatttga
     6121 tttcaattag taatgctgga agttactcct attctttatc accactatta ttttcgagtt
     6181 atgaacttcc taaacaatat tataaaagaa aagatggata tattgagtaa acaatagcat
     6241 aaattaataa acttagtatt attgaaaaaa tttaaaatgc agggggtgta attctatatg
     6301 gaattgtgga aaagaaattt atttgtatgt tggataggta tgttttttag ttctataggg
     6361 atgagccaaa tagcacctat acttccatta tatattaaac agttgggggt aactgatgtt
     6421 agtttaatac aacagtattc aggtataatt tttggatgta cctttgtagt agcagcattc
     6481 ttttcgccaa tttgagggaa agctgctgat aaatatggaa gaaaatcaat gcttttacgt
     6541 gcaagtattg ggatgggaat agttatattt accatggcgt ttgttcaaaa tgtttatcag
     6601 ttattaggat taagaatact tcaaggagtt tttacgggtt atgctactgc atgtactact
     6661 cttattgcaa cacagacaga taaaaatcat tcaggatggg ctttgggtac tcttgcaact
     6721 gcatcaacta ctggctcatt aattggtcca acagtaggag gatatataga atcaatctta
     6781 ggtcttaaaa gtacatttat tataacagga ggattgttgt ttgtatcatt tatcatctct
     6841 attttatttg tagtagataa ttttaaacct aaagaaataa aagaatcaat ttctatacct
     6901 aaagaacaat taaatatatt gccaaataaa tttttaattg catctatttt tgtaaccata
     6961 ttcattactc aactagcttt atactctata gaaccaattg taaccatata tatatcacaa
     7021 cttacaaatt ttgcatcaaa tgtagcttta atagctggat tgacattttc tgcatcagga
     7081 ttagcaaatt tattagcagc tcaaaaactt ggaaaaatgt cagataaaat aggtcctcaa
     7141 aaagtattgt taatttcact tttatgggct gggattatat ttataccaca agcttttgta
     7201 aaaactgcat ggcaactgat gttgttgaga tttttattag ggttgtcagt tgcagggctt
     7261 aatccttctg ttaacagctt gcttaaaaaa attgcaccag aagaatatgt gggaaaaata
     7321 tttggatata atgcatctgc ccagtatata ggctgttcat caggagcatt tttaggggga
     7381 caaatatcag cacatttagg tattagaaca gtattttttt caacaagttt attgttgttt
     7441 atgaatgcac tttggatgta taaatttact ggattgttta ttaaagataa gactaaataa
     7501 tcatactatt tacatttcta attttatgat gtaaaataaa tattttttat gtaatttatc
     7561 atagaattat ataaaaaata actctatggt aaaaattgga aaatattatt atcgacaaaa
     7621 tctttgactt aaaaactata ttgcgatagt attaatttaa taaaagtttg agaggggcgt
     7681 aaggttatga caatttcaaa aataccacaa gcagaattaa aagttatgaa gtttatttgg
     7741 gaaaaaaatg atacagtaac atcaaaagaa actattgaag ctatggaacg aaaatatggt
     7801 tggaaacaaa caactacatt gacactttta tcaagacttg ttaaaaaaca ttttttaagt
     7861 gcagaaaaaa tagatagata cactcattat acaatagttg ttggacataa ggagtattta
     7921 agtgttgaga caaaagattt tttaagcaat atacacgaca attctttaca gagtttaatt
     7981 tcagcactac atgatgatga agttgttgat aaagatatgt tggatttatt tgaaagtcat
     8041 tttaaaaatt tagaagaaga gtaattatat caggataaaa ctctaagaat ttaattaatc
     8101 tatggctgaa aaccataaat ggtattattt aatcagctac tcactattca taaagtaata
     8161 ttaaataggt tctctaatta gtaaaatacc taaaataatt ggaataggga gtatcaagtt
     8221 aaatgatacc acttatttaa ataagaaatg atttttaaaa tttaaaataa ttttataact
     8281 tgtcaatatt cattaatcta aagtattatt tctatttaac catatcaaaa tatattttac
     8341 tatttttaat tattacaata taaacctgtc ttttgttatt ataggtaaac taaaagtaag
     8401 aaatgatttt tatattttta aactttatta ttggtaactt atctgtaaat attttaaaat
     8461 gtactataga aagttgttaa aaatattaaa taattatatt aaatttaagt gtgtttttta
     8521 tgctataatt agtatgtatt ttttatgata tattttataa aaaatataat ttaatcattt
     8581 ttgaggatat tattatgtta gatgcattta taacaattgt ttcttctatt atagaaatat
     8641 ggtcatgtaa aaaagttttt gattatacta gtcagtcaaa gaccgactta aaaaaactaa
     8701 atatgtattt tctcttttct gtttttctta ttatttcttc tttatatata aatataggag
     8761 ctaatagtag aatcttgata tgtatatttg taatgttcgt tttctataag tctaactata
     8821 aggttaatat atataaatgt ctggtaatct cattttttta ttggttaata ttaataggta
     8881 taaatggatt aagtatgtta ttagtagcaa cagtaagcaa tttggataat atgaatgttt
     8941 tacttagtgg cagtttatat agatatgaag caatattctt gggaaagcta atgttaggta
     9001 tattacttta tgtttataag gcttctttta aaaattttaa aataagtaaa aaagagatat
     9061 acatatgtat tcctatgata gcaaatttat tgtcattttt tgtactttat gattatatta
     9121 ttaatcttgt aaaaatctct cctgaaagaa gaactgcttt tttagtaata gttttactat
     9181 taatattatc taacatttca atctttg
//
LOCUS       DAEMHW010000148         9197 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEMHW010000148 DAEMHW010000000
VERSION     DAEMHW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9197
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.148"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(115..2205)
                     /locus_tag="KPK67_002861"
     CDS             complement(115..2205)
                     /locus_tag="KPK67_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBG2459798.1"
                     /translation="MILVIAEKPSVAQTIAAVLGAKEKKDGFLTGSGYIVSWCVGHLV
                     GLAEAAAYGEQYKKWSYDSLPILPQEWKYTVAADKEKQFKILKELMHRADVSEIVNAC
                     DAGREGELIFRFVYEMAGCKKPMRRLWISSMEDSAIKEGFSCLKNGEEYDALFASALC
                     RAKADWLIGINATRLFSVLYNHTLNVGRVQTPTLKMLVDRDTAITTFKKETYYHVRLA
                     LSGAEAASEKLSDRSEADRLKAACEASKAVCTSLVKDKKTAAPPKLFDLTSLQREANR
                     LFGYTAKQTLDLAQALYEKRLLTYPRTDSSYLTDDMGDTAAGIIKLLCGKFSFMAGKD
                     FTPELGKVLNSKKVSDHHAIIPTMELAKTDLAALPESEKNILTLAGARLLMATAASHT
                     FEAVTAVFECAGQSFTARGKTILCEGWKEIYRRYRAALKSKPETDDADRDTEKTLPPF
                     TEGQTFENPTATVTEHDTTPPKPHNEASLLSAMERAGSEDTDPDAERKGLGTPATRAA
                     VIEKLVKGGFVERKGKQLLPTKDGINLVCVLPDTLTSPQLTAEWENNLTQIAKGKADP
                     TAFMEGIEDMARELVKTYPFLSEADKGRFKEEKPELGKCPRCGSPVYEGKKNYYCSNK
                     DCIFTMWKNDRFFEERKVTFTPKIAAALLKSGKVNVKKLYSPKTGKTYDGTIVLADTG
                     GKYVNYRIELPKKK"
     gene            complement(2328..2801)
                     /locus_tag="KPK67_002862"
     CDS             complement(2328..2801)
                     /locus_tag="KPK67_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2459799.1"
                     /translation="MDYIIREMRSEEYCLLSDFLYEAIYIPEGIEPPPKSVIECPELQ
                     EYIVEFGNRKHDKALVAEMQGNVIGAIWVRIMNDYGHIDNDTPSLAMSVHKKYRGLGI
                     GTSLLKQLLQVEKLAGYSKISLSVQKSNYAVKMYEKVGFTIVDENKEEYIMIVNL"
     gene            complement(2824..3072)
                     /locus_tag="KPK67_002863"
     CDS             complement(2824..3072)
                     /locus_tag="KPK67_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBG2459800.1"
                     /translation="MEDKKILLDNIDKIHTTEMGIDRIKRNLKIDTADVVEYCKNKVL
                     DKNCNIYKQGKNWYCEVENIKITINSYSYTIITAHIVK"
     gene            complement(3344..4072)
                     /locus_tag="KPK67_002864"
     CDS             complement(3344..4072)
                     /locus_tag="KPK67_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBG2459801.1"
                     /translation="MKNKLKKTMTALCAALILMGGFSVPAYAQGAATEPPAGDATNDS
                     NVVVEEKEETPPLTPKGNATLVDDFGGNKQLITVTTKSGNYFYILIDRAAEGEQTVHF
                     LNQVDEADLMALTEKGEAAKKPEICNCTEKCEAGKVNTSCPVCVTSMTSCTGKEPTPT
                     PTEQPEEPKEKGGNPGVVLALVLFALLGGGAAFYYFKFLKPKQNVKGDTDLEDFEFED
                     YDEDEPEADTGETPEDEETEEETL"
     gene            complement(4062..4331)
                     /locus_tag="KPK67_002865"
     CDS             complement(4062..4331)
                     /locus_tag="KPK67_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBG2459802.1"
                     /translation="MANNKIDRINKEIAKTREKITEYQNKLKGLEAQKTEAENLEIVQ
                     LVRAMRLTPQELNAMLSGGGIPGMNAAHATEAADYEEQEENADEE"
     gene            complement(4363..6096)
                     /locus_tag="KPK67_002866"
     CDS             complement(4363..6096)
                     /locus_tag="KPK67_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBG2459803.1"
                     /translation="MTRDGLIEVNETQQTAERISKREQDADFQKSPEQQAPQDGTQLQ
                     GVASQTAPLPHAPGAAPARDTATAERVMEHIEAAQTRKSSKKAVRKAQEEATAQTTSS
                     RLQFTEEELAERGAKYGARKLKQGYRSHKLKPYREAAKAEKAAFKANVNFQYHKTLHD
                     NPQLTSNPLSRFMQKQQIKRQYAKAAKKGGAKAAAKATRKTAKKTAEETRKAAAFAAR
                     HPAGILIAVAALLLFIMISAGLSSCGAMFSGLTNGVLGTSYTSEDSDLVATEQNYAAM
                     ENALQSEIDNIESTHPGYDEYRYDLDSIGHNPHELASYLTALLQSYTPQSAQAELDRI
                     FEKQYTLTLTEEVEIRYRTETSTDPETGETTTEEVPYEYHILNVKLENKPISSFVSEL
                     LTPQQLEMFHVYLETSGNKPLIFGGGSPDGSPSEDLSGVEFVNGTRPGNQELMELAKQ
                     QVGNVGGYPYWSWYGFNSRVEWCACFVSWCYNQAGKSEPRFAGCEWQGVPWFQSRGQW
                     GARGYENIAPGDAIFFDWDLDGVADHVGLVLGRDGSRVYTVEGNSGDACKIKSYDLNY
                     QCIKGYGLMNW"
     gene            complement(6146..8581)
                     /locus_tag="KPK67_002867"
     CDS             complement(6146..8581)
                     /locus_tag="KPK67_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2459804.1"
                     /translation="MSKGQTKKTREAAGNRRKLTRAEKKQIAEAIRKAKGDGKACTAQ
                     QTIPYLAMYPDGICKVTEKKYSKCVVFEDINYQLAQADDKTAIFENWCDFLNYFDASV
                     SVQLSFINQGTQREQAEKAISIPAQEDAFNSIRTEYSDMLKNQLSKGNNGLVKHKYIT
                     FTVEADNKAAAKSRLSRIEIDVLNNFKVLGVTARPLSGYERLKVLHGVFHPEGEPFSF
                     SFDWLIPSGLSTKDFIAPSSFRFGEGRYFRMGKKIGAASFLEILAPELNDRMLADILD
                     LETGVIVNLHIRSIDQSEAIKTIKRKITDLDKMKIEEQKKAVRSGYDMDIIPSDLATF
                     GSEAKNLLQDLQSRNERMFLLTFLVVNMADTKRKLENDIFAAAGIAQKYNCRLTRLDY
                     QQEAGLLSSVPIGENLIPIQRGLTTSSTAIFIPFITQELFQTGQALYYGLNALSNNMI
                     LCDRKQLKNPNGLILGTPGSGKSFAAKREMTNAFLITDDDIIICDPEAEYFSLVQRLN
                     GQVIRLSPTGRGINGKPQYVNPMDINLNYSEDDNPLALKSDFILSLCELVIGGKEGLQ
                     PVDKTVIDRAVRNVYRPFLADPDPAKMPILGDLYDELLRQPEPEAARIAAALELYVSG
                     SLNVFNHRTNVELSNRLVCFDIKQLGKQLKKLGMLIVQDQTWNRVTINREEKKATRYY
                     MDEFHLLLKEEQTAAYSVEIWKRFRKLGGIPTAITQNVKDLLASREVENIFENSDFVL
                     MLNQAQGDRTILAKQLNISPQQMKYVTHTEAGEGLIFYGNVVLPFVDRFPKDTELYRV
                     MTTKPEEVSESGK"
     gene            complement(8529..8933)
                     /locus_tag="KPK67_002868"
     CDS             complement(8529..8933)
                     /locus_tag="KPK67_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBG2459805.1"
                     /translation="MAYVPVPKDLSKVKTKVAFNLTKRQIVCFAAALLIGLPLFFLLK
                     GSAGTNLAAMVMIVVMLPCFLLAMYEKHGQPLEVVVRNVIQTKFTRPKERPYRTENLY
                     AVLEKQRNLEKEVSAIVKRTNKKDAGSRRKQA"
     gene            complement(8955..9194)
                     /locus_tag="KPK67_002869"
     CDS             complement(8955..9194)
                     /locus_tag="KPK67_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459806.1"
                     /translation="MTNREWGQIGNNYLKGLFALGFQGFLIMICVGIYAVLVNDMIIA
                     DNLHSAVFSLAAYTVILCFSLFKSGTLAKSIFNAH"
BASE COUNT         1981 a         2280 c         2204 g         2732 t
ORIGIN      
        1 tcggtttcag agggtttggg aaacttccca acaagcaaaa atccccgccg ttctcggtag
       61 agaaagcagg gattttacgg aaaagggtac tcttttccta tgcttgctat tcagttattt
      121 tttcttcggc agttcaatgc gatagttgac atatttcccg cccgtatcag ccaatacgat
      181 agttccgtcg taagttttgc ctgtctttgg ggaatagagc tttttcacat ttaccttgcc
      241 ggattttaag agtgcagcgg caatctttgg ggtaaaggtt acttttcgtt cttcaaagaa
      301 acggtcattt ttccacatgg taaagataca gtccttgttg ctgcaatagt agtttttctt
      361 tccctcatag acaggagaac cgcagcgggg acatttccca agctccggct tttcctcttt
      421 gaaacgcccc ttgtcggctt cggaaagaaa cggataggtc tttaccagtt cccgcgccat
      481 atcctcgata ccctccataa atgcggtagg gtctgccttg cctttggcaa tctgcgtcag
      541 attgttttcc cattctgcgg taagctgcgg gcttgtcaag gtgtccggca ggacgcacac
      601 aaggttgata ccgtcctttg tgggaagtag ctgcttgcct tttcgctcca caaagccgcc
      661 ctttaccagt ttttcaatga cggcggcgcg ggtggcggga gttccaagcc ctttgcgttc
      721 tgcgtccggg tctgtgtcct cgcttcctgc gcgctccata gcagagagca gcgacgcttc
      781 gttgtgaggt tttggcggtg tggtgtcatg ctccgttacc gttgccgtcg gattttcaaa
      841 ggtctgtccc tcggtaaatg gcggcagggt cttttcggtg tccctgtctg cgtcgtccgt
      901 ttcgggcttg ctctttaagg ctgccctgta tcttcggtaa atctctttcc aaccctcaca
      961 aagaatggtc ttgccccttg cggtaaagga ctgtccggca cactcaaaaa ctgctgtaac
     1021 cgcttcaaag gtatgcgatg cagcggtcgc cataagtaga cgcgccccgg caagggtaag
     1081 gatatttttt tcactttccg gcagcgcggc aaggtcggtc tttgcaagct ccatagtcgg
     1141 gataatagca tggtggtctg ataccttttt actgttcagc acctttccaa gctccggcgt
     1201 gaagtctttt cccgccataa aagaaaattt cccacaaagc agcttgataa tgcccgccgc
     1261 tgtgtcgccc atgtcgtcag tcagatagct gctgtctgtt ctcggataag taagaagtct
     1321 tttttcatac aaggcttgtg caaggtcaag ggtctgcttt gcggtataac cgaacaggcg
     1381 gtttgcttcc ctctgtaaag aagtaaggtc aaagagcttc ggcggggctg cggtcttttt
     1441 atctttcaca agggaagtgc agactgcttt tgacgcttca caagcagctt tcagcctgtc
     1501 agcttcagaa cggtcagaca gtttttcgct tgcagcttcc gcgccggata aggcaaggcg
     1561 cacatggtag tatgtttctt tcttgaatgt ggtaatcgct gtgtcccggt caacaagcat
     1621 tttgagggtc ggggtctgta cgcgccccac gttcaaggta tgattgtaaa ggacagagaa
     1681 aaggcgggtg gcgttgatac cgattagcca gtcagccttt gccctgcata atgcggaagc
     1741 aaagagcgcg tcgtattcct cgccgttctt cagacaggaa aagccctctt tgattgcgct
     1801 gtcctccata gaggaaatcc aaaggcggcg cataggtttc ttgcagcccg ccatttcata
     1861 gacaaagcgg aaaatgagtt ctccctcacg ccctgcgtcg caggcattga cgatttcgga
     1921 aacgtctgcc cggtgcataa gctcctttaa gattttgaat tgcttttcct tgtcggcagc
     1981 aacggtgtat ttccattcct gcggcaaaat cggtaagctg tcatagctcc actttttata
     2041 ctgctccccg taagcggcag cttccgcaag ccctaccaaa tgtcccacgc accaagaaac
     2101 gatatagccg cttcctgtga gaaatccgtc tttcttttcc tttgccccaa gtacggcagc
     2161 gattgtctgc gccacactgg gcttttctgc gataactaaa atcaaaaata aatcctcctt
     2221 tcgggtaaaa gaaaagagca accgattttt cgattgctcc tacaagtgat tgtataaaat
     2281 tgtgcgatat gaaatagtaa aataatttga caaactgtag ctttcggtta tagatttaca
     2341 atcataatat attcctcttt attttcatca acaatagtaa atcccacttt ctcgtacatc
     2401 ttgactgcat aattactttt ctgaacggat aaggatattt ttgaatatcc agccaatttt
     2461 tctacctgta atagttgttt caataacgaa gttccaatgc ctaatcctct gtattttttg
     2521 tggacagaca tagcaagcga tggagtatca ttatcaatgt gtccataatc attcataatt
     2581 cttacccaaa tagctcctat tacatttccc tgcatttctg caactaaggc tttatcgtgt
     2641 tttcgatttc caaactcaac gatatattct tgtaattctg gacattctat tactgattta
     2701 ggtggcggtt caattccttc tggaatataa atcgcttcgt acaaaaaatc acttaataaa
     2761 cagtattctt cacttctcat ttctcgtatt atatagtcca taatttcact ccaaagtccg
     2821 attttactta acaatatgtg ctgtgataat tgtataacta tatgaattga tagtaatttt
     2881 gatattttca acttcgcaat accaattttt accctgtttg tatatattgc aatttttatc
     2941 taacacctta tttttgcaat actcaacaac atctgctgta tctattttta ggtttctttt
     3001 aattctatca attcccattt cggttgtatg aattttatca atattatcaa gcaatatctt
     3061 tttatcttcc attctttctt ttcctcaact tcaaatttgt ttaactctgt catttcctat
     3121 ggttggaaca attatatcat attccatatc gcaagtatat ctttttatcg ttctgtcggt
     3181 tgtttgtttt caatctttct gatacagtac cccacacaag gggactgtcc cttataaggg
     3241 caggaagcac aagccggggg ataaggaaca ggcggcgcat tattacgccg cccgttcggt
     3301 ttctgtatca tcatcttttc aaagggattg tcggtaaaca gggtcatagg gtttcttcct
     3361 ccgtttcttc atcttcggga gtttccccgg tatctgcttc cggctcgtcc tcgtcgtagt
     3421 cctcaaattc aaaatcttca aggtcggtat cgccctttac attctgcttc ggctttagaa
     3481 acttaaagta gtagaacgct gcgccgccac caagtagggc aaagaggact aaggcaagca
     3541 ccacaccggg attgccgcct ttttctttcg gttcttccgg ctgctccgtt ggtgttgggg
     3601 tgggttcttt gcctgtgcag cttgtcatac tggtaacaca taccgggcag gacgtgttta
     3661 cctttccggc ttcgcatttc tccgtacagt tacagatttc cggctttttg gcagcttcgc
     3721 ctttttcggt cagtgccata aggtcggctt cgtccacttg attaaggaaa tgtacggtct
     3781 gttcgccctc ggcggcgcgg tcaatgagga tatagaaata gttgccgctt ttggtggtaa
     3841 cagtgataag ctgcttattg ccgccgaaat cgtcaaccag tgttgcgttt ccctttgggg
     3901 taaggggcgg cgtttcctct ttttcctcca caactacgtt gctgtcgttg gtcgcgtccc
     3961 ctgcgggcgg ctctgtggct gcgccctgtg cataggcagg gacagaaaaa ccgcccataa
     4021 ggataagggc ggcacaaagg gcagtcatgg tctttttcag tttattcttc atctgcattt
     4081 tcctcctgtt cttcgtagtc tgccgcttcg gtagcgtgtg cggcgttcat gccgggaata
     4141 ccgccgccgg acagcatagc gttaagttcc tgcggggtca gacgcatagc tcgtacaagc
     4201 tgcacgattt caaggttttc cgcttcggtt ttctgcgctt ccagtccttt taacttgttc
     4261 tgatactctg tgattttctc gcgggtcttt gcgatttcct tattgatacg gtcgatttta
     4321 ttgtttgcca taggtcattt ctcctttctt tttggggctg ctttaccagt tcatcagccc
     4381 gtagcctttg atacattgat agttaaggtc atagctcttt atcttgcagg cgtcgccgga
     4441 attgccctca acggtataga cgcggctgcc gtccctgcca agcacaagcc ccacatggtc
     4501 ggcaacgccg tctaaatccc aatcgaaaaa gatagcgtcg cccggtgcga tattctcata
     4561 gccgcgtgcg ccccattgtc cccttgactg aaaccaaggg acaccctgcc attcgcagcc
     4621 cgcaaagcgc ggttcgcttt ttccggcttg attgtagcac catgatacaa aacaggcgca
     4681 ccattccacg cggctgttaa agccgtacca agaccagtaa gggtagccgc ccacatttcc
     4741 gacttgctgc tttgcaagct ccataagttc ttgatttccg gggcgcgtac cgtttacaaa
     4801 ctccacgccg cttaaatcct cggacgggct tccgtcggga gaaccgccgc cgaaaatcag
     4861 cggtttgttg ccgcttgttt ccaagtacac atggaacatt tcaagctgct gtggcgttag
     4921 aagctccgat acaaaagagg aaatcggctt gttttccagt ttcacattta agatgtgata
     4981 ctcataaggg acttcctcgg tggtggtttc cccggtttcc gggtcggtgc ttgtttcggt
     5041 gcggtagcgt atttccactt cctcggtcag tgtcaaggtg tactgttttt caaagatacg
     5101 gtcaagctct gcctgtgcgc tctgcggggt gtagctctgc aagagggcgg tcaagtaaga
     5161 cgcaagttcg tgggggttat gcccgatact gtcaaggtca tagcggtatt catcatagcc
     5221 ggggtgggtg ctttcgatat tgtcaatctc actttgcagg gcattttcca tagcggcgta
     5281 gttctgttcc gttgccacaa ggtcgctgtc ctcggacgtg taggacgtgc caagcacccc
     5341 gtttgtcaag cccgaaaaca tagccccgca agaggacagc cctgcggaaa tcataataaa
     5401 gagaagcagc gcggcaactg caatcaaaat gcccgccggg tgtcttgccg caaatgcggc
     5461 ggctttcctc gtttcctcgg cggttttctt cgccgtcttt cttgtggctt tcgctgcggc
     5521 ttttgccccg cctttctttg ccgcctttgc atactggcgt tttatctgct gtttctgcat
     5581 gaaacgggaa agcgggttag aggtaagctg cgggttgtcg tgcagggtct tgtgatactg
     5641 gaagttgacg tttgccttaa atgctgcctt ttctgccttt gccgcttccc ggtagggttt
     5701 gagcttatgg ctgcggtagc cctgttttaa cttccgcgcc ccgtatttcg cgccgcgctc
     5761 ggcaagttct tcctcggtaa attgcaagcg ggaagatgtt gtttgtgcag tggcttcctc
     5821 ctgtgccttg cgtaccgcct ttttactgga tttgcgggtc tgcgctgctt cgatatgctc
     5881 cataacgcgc tctgctgtgg cggtgtccct tgcgggggca gctcccggcg cgtggggtaa
     5941 cggggcagtt tgggacgcta caccttgaag ctgcgtcccg tcctgtgggg cttgctgctc
     6001 cggggacttt tgaaaatccg cgtcctgttc ccgtttactg atacgctcgg cggtctgttg
     6061 tgtttcgttt acctcgataa gcccgtcgcg ggtcattttc tgcgtgattt tatcgcggga
     6121 tttatattgc ttcatggtct gcacctcact ttccgctttc ggaaacttcc tccggcttcg
     6181 tcgtcattac cctgtaaagt tcggtatctt tcgggaagcg gtctacaaaa ggcagcacta
     6241 cattcccata gaagataagc ccctcgcccg cttcggtgtg ggtaacatac ttcatctgct
     6301 gcggactgat atttaactgc tttgcaagga tagtacggtc gccctgcgct tgattgagca
     6361 taaggacaaa atcgctgttt tcaaagatat tttccacctc gcggcttgcc aaaaggtctt
     6421 tgacgttctg cgtaatggct gtggggatac cgcccaattt acggaaacgc ttccaaatct
     6481 ccacgctgta agcggcggtc tgttcctctt ttaagagcaa gtgaaattcg tccatatagt
     6541 agcgggtcgc ctttttctct tcccggttta tcgttaccct gttccatgtc tggtcttgca
     6601 caatgagcat acctaacttt ttgagctgct ttccaagctg ctttatatca aagcagacaa
     6661 ggcggttaga aagttccacg ttggttctgt ggttgaatac gttaaggctg ccggaaacat
     6721 acagctccaa tgccgccgcg atacgggcag cttccggctc cggctgtctt aacagctcgt
     6781 cgtaaaggtc gcccaagata ggcatttttg ccgggtcggg gtctgcaaga aacggacggt
     6841 acacatttct tacagccctg tcaatgacgg tcttatcgac aggctgcaag ccctccttgc
     6901 cgccaataac aagctcgcag agggaaagaa taaaatcact tttcagagca agggggttgt
     6961 cgtcctcgct gtaattaagg tttatatcca tagggttcac atactggggt ttgccgttaa
     7021 tgcccctgcc cgtaggggaa agccgtatca cttgcccgtt tagacgctgc acaagggaaa
     7081 aatactcggc ttccgggtcg cagatgataa tgtcgtcgtc ggtaatgagg aaagcatttg
     7141 tcatttccct ttttgccgca aaggattttc cgcttcccgg cgttcccaag ataagcccgt
     7201 ttgggttttt gagctgcttg cggtcgcaga gtatcatgtt gttagaaagg gcgttcaagc
     7261 catagtaaag ggcttgcccc gtctgaaata actcctgcgt gataaaggga atgaaaatag
     7321 cggtgctgct cgttgtcaaa cctctctgaa tagggataag gttttcccca atcggcacac
     7381 tggataaaag ccccgcttcc tgctgatagt caaggcgtgt caggcggcag ttgtatttct
     7441 gtgcgatacc cgccgccgcg aaaatgtcat tttccagttt ccgcttcgtg tctgccatgt
     7501 ttaccacaag gaacgtaagg agaaacattc gctcgttgcg gctctgtaaa tcctgcaaca
     7561 agttctttgc ttcgctgcca aaggtggcaa ggtcggacgg gattatatcc atgtcatagc
     7621 cgcttctaac cgcttttttc tgttcctcaa tcttcatctt gtcaaggtct gtgattttgc
     7681 gcttaatggt cttgattgct tccgactggt cgatactgcg gatatgcaag ttgacgatta
     7741 cgcctgtttc caagtccagt atgtcggcaa gcatacggtc gttgagttcc ggcgcaagga
     7801 tttcaaggaa gctcgccgcg ccgattttct tccccatgcg gaaatatctg ccctcgccaa
     7861 aacggaaaga ggacggggcg ataaaatcct ttgtagacag ccccgacggg ataagccagt
     7921 caaaggaaaa ggagaacggc tcgccctccg ggtggaatac cccatgcagc acctttaagc
     7981 gttcatagcc ggataagggg cgggcagtta cgccaagcac cttgaaattg ttaagtacgt
     8041 cgatttcgat acgggaaagc cttgactttg cagccgcctt gttatcggct tcaacggtaa
     8101 aggtaatgta tttgtgcttc acaagcccgt tgttgccttt actaagctga tttttcagca
     8161 tatcggaata ctcggtgcgg atagagttaa aagcgtcctc ctgtgccggg atagagattg
     8221 ctttttctgc ctgttcccgc tgcgtccctt gattgatgaa tgaaagctgc acgctcacgc
     8281 tcgcgtcaaa gtagttgagg aaatcgcacc agttttcaaa aatggcggtc ttatcgtctg
     8341 cctgtgcaag ctgataattg atgtcctcga acacgacgca ctttgaatat ttcttttctg
     8401 taaccttgca gataccgtcc gggtacatgg caaggtaggg aatggtctgc tgtgcggtgc
     8461 aggctttgcc gtcgcctttt gcctttcgga tagcttcggc aatctgtttc ttttcggcgc
     8521 gggtcagctt acgcctgttt cctgcggctt cccgcgtctt ttttgtttgt ccttttgaca
     8581 atcgctgata cctccttttc taagtttcgc tgcttttcca agacagcgta taggttttcg
     8641 gttctgtaag gtcgctcctt tgggcgggta aattttgtct gaatgacgtt tcttaccacc
     8701 acttcaaggg gctgtccatg cttttcatac atggcaagca ggaaacaggg gagcatgacg
     8761 acaatcatca ccatagccgc aaggttcgtc cctgcgctgc ctttgagcaa aaaaaacagc
     8821 ggcagtccaa ttaagagtgc cgccgcaaaa caaacgattt gccgctttgt cagattgaaa
     8881 gcgacttttg ttttgacttt ggataaatct ttgggtacag gtacatacgc caagaaaaaa
     8941 cctcctttct gcccttagtg ggcgttgaat atggattttg ccagtgtgcc ggatttgaac
     9001 agggaaaaac agaggataac cgtataggcg gcaagggaaa ataccgcgct gtgcagattg
     9061 tctgcgatta tcatatcgtt taccagtacg gcgtaaatac cgacacatat cataatgagg
     9121 aagccttgaa aaccaagagc gaacagccct tttaggtagt tgtttccaat ctgtccccat
     9181 tctctgttgg tcatggt
//
LOCUS       DAEMHW010000149         9169 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEMHW010000149 DAEMHW010000000
VERSION     DAEMHW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9169
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.149"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..611
                     /locus_tag="KPK67_002870"
     CDS             <1..611
                     /locus_tag="KPK67_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2459807.1"
                     /translation="THNMDVEFISYMWIIATPVSSIIMGNLNKSINELQNTNKKLSEQ
                     YKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTDFSLMIVKLPYYGNLQTIFGENK
                     TNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPNTSLEGSKVVKDRIKKRIKELNL
                     DLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEEELQYDV"
     gene            601..2265
                     /locus_tag="KPK67_002871"
     CDS             601..2265
                     /locus_tag="KPK67_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2459808.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKSISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            2314..3570
                     /locus_tag="KPK67_002872"
     CDS             2314..3570
                     /locus_tag="KPK67_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG2459809.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            3593..5674
                     /locus_tag="KPK67_002873"
     CDS             3593..5674
                     /locus_tag="KPK67_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBG2459810.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPESTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQKALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDKSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLRGDTLIIDEYGEVEDLAYNLKPKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            5711..6796
                     /locus_tag="KPK67_002874"
     CDS             5711..6796
                     /locus_tag="KPK67_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG2459811.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            6850..7974
                     /gene="wecB"
                     /locus_tag="KPK67_002875"
                     /pseudo
     CDS             6850..7974
                     /gene="wecB"
                     /locus_tag="KPK67_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
     gene            8052..9026
                     /locus_tag="KPK67_002876"
     CDS             8052..9026
                     /locus_tag="KPK67_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG2459812.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQSLENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         3848 a          844 c         1500 g         2977 t
ORIGIN      
        1 ttactcacaa tatggatgtt gaatttatat catatatgtg gataatagca acaccagtat
       61 cttcaattat aatgggtaat ctgaacaaaa gcataaatga attgcaaaat acaaataaaa
      121 aactatctga acagtataag gaattagtga ctattgatag tgaaactgga cttagaaatt
      181 taaagatttt ttataatgat gtaaatatgg agataagtaa atctattaga cataatactg
      241 atttttctct tatgatagta aaattaccat actacggaaa cctacagact atatttggtg
      301 aaaacaaaac taataaaata gtaaaatata ttggttcaaa tattatagaa tgtacaagaa
      361 atgaagatat aatttatagt ttacaaaaag atatgatagg gatactaatg ccaaatacat
      421 ctttggaagg ttcaaaagtt gttaaagaca gaataaagaa aaggataaaa gaattaaatt
      481 tagatttaaa caatagggga aaatatgtaa atatagatgt caaaattgca tttttacaat
      541 ataagagtag ttttggagat agtataaatt ttaagaatat agttgaagag gaacttcaat
      601 atgatgttta aaatatttaa taaaagtcta ttaaaattag tagttgtatt atcaatagta
      661 ctgacaatat caaattttag cttatcttca tatgcatcag aaattacttc cagcacagat
      721 aaggtattaa tcatatatga ttctaagaaa gaaacagctt ataatagaga tattttaaat
      781 attatgagga ctctattggg aagatttagt tcagatatag agctattaaa attgagtaac
      841 tatgatggtg agataaataa aaattattac agtcatatat ttattttagg aatcaatgaa
      901 aactcatata ataatgataa aaacactaaa aacttgatta gttccttaaa ttcatataaa
      961 ggaactatat gttggttagg atatggtata gaaaatcttt tggaacataa aaaatacaat
     1021 ttagactatg taggaaaaac aaacaatata gttagtgtaa attatagagg taaatcatat
     1081 aacttggatg aacattatgt ttttaacatt gtagaatcaa aagatacaag taataaagta
     1141 ataggaagta ttaatgatac cttaaataag tatccatata taataaatga taaaaatctt
     1201 ttttatgtat caaaattaga tttagatgga gttttatttt atatattttg cgattcactt
     1261 aatgatattt ttaatattaa gacgtttgat aaaggacgta tatttgtaag aatagaagat
     1321 gtacatgctt ttagagaacc aaaaaatctt gtagagatag cggattattt aagtagtaag
     1381 aatataccat ttacgattgc attaatacca gcatatgtaa atcctaaaaa tcataaggta
     1441 ataactttat cagaaagccc tgaaattgtc aaagcaataa aatatatgca agataaaggt
     1501 ggaactgtga tattgcatgg atatactcat caatacaaga aagaagaagt ttctggagaa
     1561 gggtatgaat tttgggatgg taaaaaagat gaacccttaa aagaaaatat gaagatattt
     1621 gtcaaagata gagttttgaa tggtcttaga gtatgtatag agaatggaat ttatcctctt
     1681 gcatttgaag cgccacatta tgcaatggaa tcagagggat ataaagaatt aaaaaaatat
     1741 ttttctactt atatggggca acatcaaaat aatgataaaa aatttagcac aaatacatat
     1801 ccttatatta ttagggatac agaggagttt aatatattta tacccgaaaa tttaggatat
     1861 atagacccag aagataagtt tacttttcaa aatataaaag aaaatttaga taaattatcc
     1921 attgtaagag gttttagtgg agggttcttc tttcattcat atttaaacat agagtatcta
     1981 aaaaacacaa tagaatattt agaaaaacag aatatagaat ttatgaattt aagagatttt
     2041 aataattggg taaaagttga tgaaatacaa ataagaaata atggagatga aattatagtc
     2101 aactatgata aggacttaga tgaaataact aaaagtgata ctagatttaa atccatatcc
     2161 aatattagta aaattctaat attcatagta tctataagtg ttttaatatt tgtgattata
     2221 tttatatatt ttaagagaat agataaaaag aaatttttaa aataaatgat tttatattag
     2281 aatcaatatt ttaaaatatt ttaggagaaa tttatgagag atttaaatgt atcagagtat
     2341 ttgttcgtat ttagtctgtt tagtatatgg tctttattgc ttataaatat tattttagct
     2401 atgggtggat atatatttta ttttaaaaac tttgataaag aaataaaaga aattgatgaa
     2461 tatcctatga tatcaatact tgttcctgct cataatgaag ctaaagtgat aggtagaact
     2521 gtggagtctt tgctattact caattatcct aagagtaaga tggaacttat tgttataaat
     2581 gacaattctt ctgataatag taaagaaatt ttagaaaata taaaggatag gtacaacaat
     2641 tataatttca ctataataaa tacagatagt ttaacaggtg gaaaaggaaa gtctaatgca
     2701 ttaaatattg gatatacgat ttcaaaagga gattttatag ctgtttatga tgcagataac
     2761 acaccagata aaaatgcact tagatatcta gtacaaacta tagtaatgaa tgatgaatta
     2821 ggtgctgtta ttgggaaatt taggactcga aataaaaata aaaacctatt aaccaaattt
     2881 ataaacatag aaacattaag ctttcaatgg atgtcacaag ctggtagatg gcaacttttt
     2941 aatctatgta caataccagg aactaacttt atattgagga gaagtatcat agaagaaatt
     3001 ggaggatggg atagtaaagc aattgcagaa gatacagaaa taagttttag gatatataaa
     3061 ttgggataca agataaagct tgtaccacaa tctataacat gggaacaaga gccagaaact
     3121 gttaaggtgt ggataaagca acgtactaga tgggcaaaag ggaatatata tgtattaatg
     3181 aaatatataa aaaatatatt taaacaggga agaaataaga ttgtgtttga tatagcttac
     3241 tttttttctg tatattttct atttttaaca tctgtaatta tatctgatat tttatttgtt
     3301 ttaagtatat ctaaactagt agaaatttct attccaatta acttttttct aatatggata
     3361 ctatcttatc ttttatttat tatagaagtg agtataagcc ttactataga aaaaggtgaa
     3421 gcaacaattg aaaatatatt catagtggca ataatgtatt ttacatactc tcaactatgg
     3481 ctgtttgtag ctataaaagg aatgatagag tatctaaaag atataatttt taaaagagag
     3541 gttaaatggt ataaaacaga aagattttga ttcatgtaca ggaggaatta acatgaaaaa
     3601 atttattata tcaattatat ctttagtatt gtttttttca aatatatcac ttatatacaa
     3661 agtaaatgct gatgagacta aagttaaaaa ttataagttt gaaagagata taactattga
     3721 tggtgttata ggaagtaata gtactttttt tgaagtaaat aaaaattggg atattgaaga
     3781 agttttattg catcttaatt ttagtaagag ccaaatatta aatggagatg tttcaagttt
     3841 aactgtattg ataaataatg taccaataaa atctattaaa ttaaatgcta agacaaatta
     3901 taaaaataca ttagaagtat tagttccaaa agattatata attcaaggat ataatgaaat
     3961 aaaaataaaa acatacaaaa caatatctga taaaatttgt caagatgatt ccaatactgg
     4021 gaactggatg gttattcaca aagaatcata tatatcaatt agatataagc agaaaaaggt
     4081 ggaaaactca ataaatgaat atccgtatcc atatgcagaa attgaaaata atcataaatt
     4141 agatacaact atagtagtac cagataatat gactagagga gagactacag cagtgtttaa
     4201 tttggcatca gcatttggaa aaataacaaa aaatgatgat ttaaagttag atgttaaatt
     4261 atattcagaa atgaaaaatt ggtcagatga taatattata tatattggta aacctgaaag
     4321 tacagcagaa gaaattttgg atattttaag tataaaggaa caaaccctat tatcttcaaa
     4381 ctgtataatc aagcaagtag attcaccata caataagaat aagaagatga tggtggtaat
     4441 tggtagtaat gaagatgatt taataaaggc atcaaattta ttaatagaaa atagactttc
     4501 taatcaagtt ttatcatcat cagtacttgt aaacaaagag actaatataa aaattaatag
     4561 ggaacaaaaa ttaaatttag gtcatttaac attaaaagac ttaggatatt ctgacttttt
     4621 gttggaagga gcatttaatc aaaaagcttt atttgatgtc aaaataccaa cgggtaaggt
     4681 attagatgat ggttcaaaga ttatcttaaa tttaagatat tctgacaatt tagattttga
     4741 aaaatcttta gttacagtgt ctataaatga tgttattgtg ggaagtaaaa aattggataa
     4801 gtcacattct aataatgata agttagaatt aaaaatacct aaagatatag ataataaaaa
     4861 ctattatcaa gttaaattga catttaattt gagtataaaa aattcgaatt gtgttactag
     4921 agagagtaat aatccttggg catatgtatc aaataattct tatttggcgt tatctacgaa
     4981 agaaaatgaa accttatctt ttgaaaatta tccgtatcca tttgttagag atgatgaatt
     5041 taatgattta actgtgataa tgccagatta ttcagggtca caagctatga cctggatgtt
     5101 tagattaggt gttactttag gtgcgaatat aaattcacat aatggaaata taaatgtaat
     5161 tagaggcaaa gaatttagtg acaagtataa ggatactaat attgtagttt ttggagtgcc
     5221 acataataat tctgttataa agatgttaaa taataattta aatataaagt tcgataaaaa
     5281 ttactccaat tttatatcaa atgataagat aagctttatt gatgattatg gaaaaaatat
     5341 ttctactatt caattaataa aatcaccata taataatcaa aaaaatatca tggtaattag
     5401 ttcaatgaat gaaaaaaact tatatttagg aatggattat ttgttgaata aaagtaaagt
     5461 taatgattta agaggagata cactgataat tgatgaatac ggagaggtag aagatttagc
     5521 ttataattta aaacctaaga aagaagtaaa agattcaagc tggaatatga gtataaataa
     5581 gacaactaaa gtatttttaa tgatttcttt tattactata attgtagtca tgatattatc
     5641 aatgctatat ataaaaaaat ataaaagaag ataatatgat aaatttatta ttttaaaaga
     5701 ggtatataat ttgaggaaat atatgaataa atttaaatta aaaagaagta ttttaatctc
     5761 attaataatc ttaattttta tatttatagt ttactttgta agtattgagt taaaagatag
     5821 gagtctagta aataatcaat ataaaggtaa gattaaatcg gccaatttat ctgtagatta
     5881 tgaaataaat caagttatga aggatataga taaattggga ttgaatacag taaatgtacc
     5941 tataattata aatgtagagt ccattaattc tgatattatg tcaatagata ataatagtaa
     6001 agaaaaggca attaagttaa taaaaaaatt aaataaaaaa ggtatttcaa caatattaga
     6061 ggcatatcca tggattgaaa atggaaaatt gtatgagact gactggaacc ctataaataa
     6121 aaaaagattt ttttatactt ggcaaaatgc catattaaat gaacttataa ttgatgtagc
     6181 aaatccactg gatgtaaatg ttttaaatat tggttctaat tttgtacatt tggaagaata
     6241 tcaacaaaat tggggtgaaa taatagattt tgtacaatcc aaatttgatg gtcttgtaac
     6301 gtacagaact aattggtggt atacaaaaaa agatgattta agtagtaagt tgttttatga
     6361 acaaaagttg aacaataatt tttttgataa gttagatttt atatctatag cagcatattt
     6421 tgagttaagt aataaacctg ttaatacagt ggatgaatta atttctgcct tacactcatc
     6481 tactgtaaac aatagaggac aaaatataaa acaagaaata tataaccttt ataaagctca
     6541 tagaaaacct atattttttg gagaattagg tttttcaaat agagaaagtg caagttctca
     6601 gccgtggaat catactcctt caaaagctgt aaatggcgaa gaacaagcaa ggtgttttga
     6661 ggcatataag aaagtttttg aaaatgaaga ctggataaat ggtttttctg tgttttgtgt
     6721 aggtaaaata gatgacgaaa aaaattttta cccatcaaaa gagagtataa aagttattaa
     6781 gtcttggtat gaataatttt gaaagtgtag aatttatata gattaaatag atgattagga
     6841 gagatataaa tgaatgacat caaggttatg actgtatttg gaactagacc tgaagctatc
     6901 aaagtagcac ctttaataaa ggaacttgag aaaagagaga atataaaaag tattgtttgc
     6961 gttacagctc aacatagaga aatgcttgac caagttatag aaacctttaa tataaatgta
     7021 gactatgatt tggatataat ggaaaaagga caaagcctga atgatataac atgtaagatt
     7081 ttaaataaac ttcccttgat tttaaacaag gaaaatccaa atataatatt ggtacatgga
     7141 gatacaacca caactctagc tacaagtttg actgcatttt ataataaaac tcttgttggt
     7201 catatagaag ctgggcttag aacctatgat aaatattctc cttttccaga ggagttaaat
     7261 agacaactta caggtattat tgctgatatg cactttgctc ctactaattt ggctagaaaa
     7321 aacttgattt ctgaaggaaa acctaataat aatatatttg taactggtaa tactgctatt
     7381 gatgctttaa agatgacaat aaaagaaaac tataatcatc ctataattga tgagattggt
     7441 aacgatagga tgattttgtt aacctctcac agaagagaaa atttgggtaa acctatgaaa
     7501 aatatattca gagctataaa gcgaatagta gatgactttg aagatgtaca gatagtatat
     7561 cccatacatt taaatcctaa aatatgaact atagctgatg agatatttgg aaaatttcct
     7621 gaaaaaattc atattataga gcctttagat gtagctgatt ttcataactt tttaaataaa
     7681 tcttatatga taatgactga tagtggagga atacaggaag aagctccatc attaggtaag
     7741 ccagtccttg tgttgcgtga taaaacagaa agaacagaag gaattgaagc aaagacactt
     7801 aaattagtag gtactaatga agatagaata tataattcag tgagcgattt attaataaat
     7861 aaagataact atgttcagat gagtaaagca tctaatccat atggagatgg aaatgcaagc
     7921 aaatatatag tggatataat aattaaaaaa tttaattgca aatatttaaa ttagataagg
     7981 gctaaaacat taactaattg atataagatt tatgataata ttaaggaata caatttttaa
     8041 gggggagtaa tatggctaga aaattaataa aaaatttggg taaaagtaaa agtgtaaaga
     8101 gagtgaaact tttatttaag aagattttta ttactgtatt catagtagca agtatagttg
     8161 ctatttttaa tataacgaaa tattttgagg aattatacaa agtaagggat ttaaaaagta
     8221 ctaaaattga atactatatg gatgtggctg atgaagcagg agatggaaag gtccaattaa
     8281 gttggaaggc cttacttgct atagacatgg taatacatga tgaagattta agtaatataa
     8341 aaaagaaaga cacattggat ataggggaaa agtttatagt agaagataaa aatgataaag
     8401 gcgaaaaggt gtataaggta aaaaagttta ataaggtatt aagcgaattg aaatttgact
     8461 cttctcaaaa aagtagagca agaaaataca tgaaagattt ggaatacaca taccttggaa
     8521 ataaacaatt agatagtagt gatgaaaaaa ttaaatttat aaagaagtta gaagactcag
     8581 ctataagaga atatattgat tatggaatat tgccctctat aacaattgga caagctatat
     8641 tagaatctgg ttggggaaat tctaaactta caaaacagag taataattta tttggtataa
     8701 aagcagataa agcatggaaa ggaaaaagtg tagaaatttc aacttcagag cattataatg
     8761 aaaaaattgt agctagtttt aggtcgtata attcattaca agattctgtc aaagaccaca
     8821 gtttattttt aattaataat aaaagatata gaaaacatgg gttgtttgag gcaaaagatt
     8881 atattagtca agctcaatca ttagaaaatg caggatatag tacagcagaa gataaaaaag
     8941 gaaatcgtat atatgcagag ctattaatag atgttattag gagttataat ttgcaattga
     9001 tagataataa agttgagaca aaataattgt taactttata tgaggagata acaagataag
     9061 attcttttct acaaaaagaa tagaaaaatt gtaaaaaaaa ctaaaaaaat gtattgactt
     9121 gttatttaaa aggtgatatt attattactg tactcgaaag agaacaaaa
//
LOCUS       DAEMHW010000150         9141 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEMHW010000150 DAEMHW010000000
VERSION     DAEMHW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.150"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1..384
                     /locus_tag="KPK67_002877"
                     /pseudo
     CDS             1..384
                     /locus_tag="KPK67_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            535..1341
                     /locus_tag="KPK67_002878"
     CDS             535..1341
                     /locus_tag="KPK67_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2459813.1"
                     /translation="MGMILSTLGINLYLAPAKLLSGGVAGICVILYKLFGINQGISSF
                     LMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLTQDIAKYFPIDDTMLQCIYGGAL
                     TGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNTFLFINFFVVCAGAFLFGAKLVM
                     YTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEISKVIMNKMGRGVTFLEAEGAYT
                     QNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNNVEEVKGGGFKDKFL"
     gene            1468..1740
                     /locus_tag="KPK67_002879"
     CDS             1468..1740
                     /locus_tag="KPK67_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459814.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(1903..2475)
                     /locus_tag="KPK67_002880"
     CDS             complement(1903..2475)
                     /locus_tag="KPK67_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2459815.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            2659..3501
                     /locus_tag="KPK67_002881"
     CDS             2659..3501
                     /locus_tag="KPK67_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG2459816.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            complement(3604..4680)
                     /locus_tag="KPK67_002882"
     CDS             complement(3604..4680)
                     /locus_tag="KPK67_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG2459817.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            5177..6580
                     /locus_tag="KPK67_002883"
     CDS             5177..6580
                     /locus_tag="KPK67_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2459818.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    6671..6911
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6954..7652
                     /locus_tag="KPK67_002884"
     CDS             6954..7652
                     /locus_tag="KPK67_002884"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBG2459819.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            7675..8676
                     /gene="trpS"
                     /locus_tag="KPK67_002885"
     CDS             7675..8676
                     /gene="trpS"
                     /locus_tag="KPK67_002885"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBG2459820.1"
                     /translation="MSEKKVILSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
BASE COUNT         3409 a         1084 c         1519 g         3129 t
ORIGIN      
        1 aatcaaagaa atgattttct acaaaaacta tctactaaat taataaaaga gaatgatatt
       61 atttgcatag aagatttaca agtaaagaat atgattagaa atcgtaaact atctcgctta
      121 atttctgatg tgtcatggtc agagtttatt cgtcaattag aatacaaggc taattggcat
      181 ggtagataaa ttgtaaaggt aggtaaattc tttgcaagtt cacaaatatg caataaatgt
      241 gggtataaaa acgaggaagt gaaggactta aatataagag aatggatttg tcctagttgt
      301 aatgaaactc acgatagaga tataaatgca agtataaata tattaaaaga aggattaaga
      361 ctaataacga ttcaaaataa ataacttatg taagaaccgt aggaactacg gggatagctt
      421 gtggagaatt agtaagacat attttagtat gcaaaattct atgaagcagg aaccctgtga
      481 ctttagtcat gggaggttca gaataacata tttttacaat atggtctgat tatattgggt
      541 atgattctat caaccttagg aataaattta tatctagcac cagccaagtt attaagtgga
      601 ggagtagctg gaatttgtgt tatattatac aaattatttg gtataaatca gggaatatca
      661 agtttcttga tgaacatacc aatatttatt atagctagaa agtattttga taataaattt
      721 ttgctaataa gttttgtcaa tatgttacta ttttctctag cactggggct aacacaagat
      781 attgctaaat atttcccaat agatgatact atgctacagt gtatttatgg tggtgcatta
      841 actggtatag gtatggggtt aacttttaaa gcaagagcta ctgctggagg acttgatata
      901 atagcagcca taatgaaaag aaagtatgat atacctatga aaaatacatt tttatttatt
      961 aatttttttg tagtatgtgc aggagctttt ttatttggag ctaaattggt gatgtacact
     1021 ttaattacaa tgtatataat ttcttttact atggatatag gaaaagattg ctttgataga
     1081 aaaaaatcta tacttttaat atcaaataaa tatgatgaaa tatccaaagt gataatgaat
     1141 aaaatgggta gaggagtcac ttttttggag gcagaaggtg catatacaca aaataagaaa
     1201 aagatgattt attgcataat atctgcaaat gaaataggta aatttaaaga tttaatttat
     1261 gatatggata gtgaagcttt tatttctgtc aataacgttg aagaagtaaa aggtggaggt
     1321 tttaaggata aatttcttta agattatata tgttaattat atatgttaaa aaatgccata
     1381 tgtattgaga ttatacctag ttagatgtat actgtagtta atggggtaca tgaagtactc
     1441 aataatatac taggaggtat aagaattatg ttaagagaga gatttgtagt aagaaatcat
     1501 cataatccac atacaagtgg gtgttgtggg actggaggag aaggatgctg tggaggagat
     1561 aaccatgaac attcacatca acattctcac agtcatgggc atgaacatga gcatgtaaat
     1621 ggtgtaggtc acgaccataa tcatgatgat ggtttttcag gtactcatga gcatagccat
     1681 gagcatacac atacacatgg tcatgaacat tcagatgggc atacacatga acataaataa
     1741 aaacatttta aatttataag agatttttat tttgtgattc catgaaggtt taaataaaaa
     1801 ttgaatgttt taagtaaaaa ttattgagaa taaataagaa agtgaactgt taaagtagat
     1861 aacttaaata gttcactttt attaattatc taagtattaa gtttaaatcc aatgattaac
     1921 taaaaaagaa gctatatttt cataaggtat tttttctgtt tttaataaac tttggacaac
     1981 taaaccatca actattgaca tgagtaaaaa agaaaatgat tcagcatcct gttcattttc
     2041 gctactatta tatttttcta gtgattcaac aatttcagat ttaagagtag ttctccattc
     2101 aatatatttt aatcttattc tttcctttaa atcattattt gaagtaattg cctcacaaat
     2161 aagatacata tgtaccctac cacgactttc tatagtagat attttctcta agattaagtt
     2221 aaccatttct atttgagaat tttttgattt gacatttttt acacagttta gtatagcact
     2281 agttataact tctaaatgag tatccgcaat atcatatata atatcatctt tagatgcgta
     2341 gtgataatac aaagtacctt tacttatatt tacatctttt gcaatatcag caaggctagt
     2401 attttgaact ccatttttgg ctattaaatt ggttgcagat gataaaatag cctgtttcat
     2461 attatttgtt ttcataaaac ctacctttcc atgtatattt ataacaaatt aataacatta
     2521 taagcttatg aatatttatt gtcaataaag cgagaatcta tatctattga ctttttttca
     2581 ttcaatatat aaaataataa ttaaatcggt cgtacgaccg actgaataaa taaaataata
     2641 tcttatagtg aggttaatat ggataaaata aaattgatag tggacagtgc atgtgactta
     2701 cctgatgata taatagaaaa atacaacata gaagttgttg gtttaaatgt atcttttgga
     2761 gaagaaagtt atatatctgg aaaggaaata gataatgaaa ctttctatag aaaaatgagt
     2821 gagagtaaag tactaccaaa aacatcatgt ccttctcctg ataaattttt agaagcatac
     2881 cattgtcagg aagaaagtgt attagtgatt aatataagtt caggattatc tgggacttat
     2941 aatagtgcac ttttagcaaa agatatgttt gaaaaggaag gaaataacaa gaaagttgag
     3001 gttatagatt cacttagtgg gtcaattgga ataggacaat tagtgttaaa agcagcaata
     3061 ttaataaaag aaggaaaaga aattgaagag attgtttcaa taataaataa atataaagat
     3121 aatcctccat tttttggaac acttgaaact ttagaaaatg ctattaaggg tggtagaata
     3181 aatccaatag caggaaaaat tataaatacc ttaaatttta aggcgataat acaagttgca
     3241 gatggcgttg taactcctat agataaggca agaggtgaag gaaacagttt gaaaaagctg
     3301 attactttag tagagtcaaa aattaaagat aaagaagaaa aggttttatt tataggacat
     3361 gctaattgcc cagaaaaggc tcaaaaggta agagaagtta tggaaaagga tgtaaaatac
     3421 aaagatgttg taatttgtga aattggttca gtgatgggga catatacttc gaagggtgca
     3481 atcttaataa ctgcaatata aataatagat tgaaataaat catagattga gaaattaaag
     3541 ccatataaaa atgtttaaaa catttttata tggcttttta attttatcaa attgatttta
     3601 gaattaacca actacacata tttctttgtc atgtttgttt aatatctcac aaccatcttc
     3661 agtaacaagt actaaatctt ctattctaac tccgaactca cctggtaaat atatacctgg
     3721 ctctatagag aatatcattc ctggttgtac agtatctgta tttatagaag atacatctcc
     3781 tttatcatga acttcaagtc ctatagagtg accagttcta tgagtgaagt actgtccata
     3841 tcccttttca gttatgtagt cacgagcagc agcatctata tcacagaatc taacacctgg
     3901 ttttacaata gcttcagctc tcttattagc ttcaagaact atttcaaata tttctctacc
     3961 tttttcaggt atttctttat agaatacagt tcttgtcata tcagcacaat agttatcttt
     4021 tatacaacct atatctaaga ttattgcatc tcctggtttc acaagtgcat ttccaggttc
     4081 accatgtgga ttagctccat taggtccaaa acctataata gggtcaaatg aaaatccatc
     4141 agtacctagg tcttcatata ttttagataa tttaccaaca agttgttttt ctgtaagttc
     4201 accagaaaca gtagctttaa gttgttccat agccttgtca tttaattttg aagctattct
     4261 cattaactct ttttcttctt catctttaca cattcttaaa gtatctatta tataagatga
     4321 atttacaaac ttacttcctc cacctaattc tattaagttt aataagaatc tagctggcca
     4381 gtttttatca acacccatag tagcattttt gtctatatgt tcagttatta tttctactgg
     4441 attttgagta tcattaaacc aaaccatttc aacacctaaa tcttctgaaa ctgggaataa
     4501 ttcatttata aataatttat tattgccatt taaatttaag tataatgcta atagtctttc
     4561 gcctggatgg attagtactc ctgtaagata aaatatagaa gtagggtcac ttactaacat
     4621 ttgagaaata tcatcttttt tcatttgttc aagtacagca tttaatcttt gagctttcat
     4681 aataatttcc tcctaatcaa aattcaatac aaatcaattt tagcattatt aaacaaaaaa
     4741 gagtatactt ttcttctaga aatataaaaa aaatttgtta tatatttttt atattaagtt
     4801 taatctctac ttttgcatta aatacggaag taaaatgact ttttattgtg aaaattctat
     4861 aaaagtaata aattaagtct ataaagttat gtattttatc tttttatata ctaatgaaaa
     4921 ataataaata aaattttata ataaaataat attgaaacaa ttttaaaaat ttagtatcat
     4981 tagaattaaa gtggtaaaac ttttaaattt atcattttta ataagatttg atttcaaagt
     5041 atttaaggtt tattattaaa actgtttttg aatatgcaaa tattttgaaa aatatcagtt
     5101 taataataat gaatctattt tattaatttt gaaattaaat ttaaaataat taatagagag
     5161 gggaaattaa attaggatgg aaaagaaaaa aataggccta tggagtttgg tatttatgaa
     5221 tgtatcagct ttatatggta taagatggat agctaaatct acagcatcaa gctttggttt
     5281 aggattaggt gcaataccta tgtggttatt attctcattt atatactttg taccaggagc
     5341 tttaatttgt gctgaattag cttctacata tcaaaagggt gatggaggtc tttacgattg
     5401 ggttaaggaa gcctttggag aaaagtatgg atttttagtt tcttggttaa actggacagc
     5461 taaaatattt tggtattctt catttttaac tttcttagca gtaaatgttt cttatacaat
     5521 aggaaatcca gcccttgctg ataataagat gtttgtactt atattatctt taataatatt
     5581 ctgggcatta tctcttatag caactaaagg aataagtttt acaaaaatat ttactaatac
     5641 aggtgcatta ggttcaacaa taccaagtgc tatacttata atattttcat ttgtagcagt
     5701 atttgtgttg aaaaaacatg atatagcttc tacatacaca atagcttcaa tgactccaaa
     5761 acttaatatg gatgcatttg ttgctatatc agctataatg tttggtttag caggagcaga
     5821 gactgctgct aacttcataa cagaaataga taagcctgaa aagaacttcc ctaaggcaat
     5881 attaatatca gctggaatag tagcttcatt atatgtatta ggttcaattg caataacaat
     5941 gataatacca ccagaccaaa ttactgcttc taaaggtgta ttagatgcac tttctgctgt
     6001 ttgtgcgtct ttaggaattg gttcttggtt ggttcaatta atagcttttg gtatagcttt
     6061 ttcagtatta ggagcaatag ttctttacat agcatcacca ataaaaatgt tatttggtag
     6121 tgttaaaaaa ggaatatttc cagatagttt agtagaggta aatgaacaca agataccttc
     6181 taaagcagtt atacttcaag caataatagt tactataata ctattggtga ctacattaat
     6241 gccttctgtt gatgctatat acaatgttct tgtaactatg acagcattaa ctgcattatt
     6301 cccatatgta ttgttatatg cttcttatat taaactaaga aaagagagac cagatgaaat
     6361 tagaccttat actatggcaa aatcaacaag tacttgccta ggtttagcaa aaatggtttt
     6421 agttgtaact gtagtgggta tattattatc tgcagcacca gttatgccta catttgctga
     6481 aaatgttata tatgagatag agatgattgg tggaggtctt ctagttatat tatctggtct
     6541 atggttatgg aatagatatg aaaagaaaac agctaaataa aatgtatttt aaaaatttct
     6601 tttggatatt ataatatata gttgacaatt tatagtgtaa ggaatattat aaaataaaga
     6661 aattaataaa ttaaatcctg agataagaaa tagtaaataa atatattttt gcacagagaa
     6721 ttaaggatgc tgagaactta ataaaaagta tttattgaat gggtctttga ggagtaaaat
     6781 gaaatgtaat ttaagattac agagtatttg atatcgtgta tgcacgttat agcatatatt
     6841 aagatatata tattcaattt tgagtgtata taaatttagg tggtaccacg gaaaatatat
     6901 ccgtcctatt gatttaggac ggatttttta atatagaaaa tttaggagga aaaatgaacg
     6961 taataggaat tataggtgca atggatgaag aagttagtat tttagttgat ttaatggata
     7021 ttagagagac tataaaaaaa gctagcttag aattttataa gggaattttg gaaggtaaga
     7081 atgtagtgtt agtaaagtgt ggcataggta aggttaactc tgctctttgt gcccagatac
     7141 taataagcga atttaaggtt gatgctattg ttaatacagg tgttgcagga gctttaaacg
     7201 aaaaacttga tgttaatgat atagtaatat caactgatgc tatacagtat gatgtagaca
     7261 ctacagcatt tggagaccct aagggagtta taccaagaat gaagacttct gtatttaaag
     7321 cagatgaaag acttatagat gctgcttaca aatcttctgt ggaagaagta aagactcata
     7381 aagtattaaa aggaagagta gttacaggag ataaatttat aaattctaaa gaattaaaag
     7441 aagaattagt aaatgatttt ggtggatatt gtggagaaat ggaaggtgga gctatagctc
     7501 atgtatgcta tttaaataat actccatttg taataataag agcaatgtct gataaggcag
     7561 atggaagtgc tgatgttact tatgatgtat ttgtacatga tgctgccaat aactctaaag
     7621 acatagtttt aaatatgtta aaatcaatat aaatatataa ggaggtacaa aaaaatgagt
     7681 gaaaaaaaag ttatattaag tggtgcacag ccatcaggca aaatgacttt aggaaattat
     7741 ttaggagcta taaaaaactg gactgaactt caagacaatt atgattgtta ttatagtata
     7801 gttgatttgc atgcaataac agtacctcaa gaccctaaag ttttaagagc caatactata
     7861 gaattacttg ctcaatatat agcttgtgga ttagacccag aaaaaaatac tatatttata
     7921 caatcacatg tcaaagagca tgttgaactt atgtgggtat tgaatactat gacttatatg
     7981 ggtgaattaa gtagaatgac tcaattcaaa gataaatcac aaaagagtga agcaaatcta
     8041 aatgcaggtt tattcacata tccagttctt atggctgctg acatattgtt gtatcaaact
     8101 gacctagttc cagttggaga tgaccaaaaa caacatttag aattagctcg tgatttagca
     8161 aataggttta ataatagatt ttcacctact tttgtagttc cagaaggata ctacccaaaa
     8221 ggtggagcaa gagtgatgag tttgcaagag ccaactaaaa aaatgagtaa atccgattca
     8281 aatgaaaatg catttatact attagcagat gattcagatt ctataaagag aaagataaaa
     8341 agaagtgtta ctgattcttt aggtgttgta aaatacaatg atgaacaacc aggtattaaa
     8401 aatctaattg atatatattc taatttaagt aaaaagagtg tagaagagat tgtaaacatg
     8461 tatgaaggaa agggttatgg catatttaaa gaagatgtgg ctgaagtagt atcagaagct
     8521 ttaagaccta taagagaaaa atatgttgac cttcttaata ataaagatta tttagaaaaa
     8581 atatatagta tgggagcaga aaaagccgaa aaacaagcta gaaaaactct tagaaaagtt
     8641 tataaaaaag ttggatttat agagagaaga tactaattat tagctatctt tacttgataa
     8701 ataaatttta aagttattaa aaaggtatat gatatgaaag agagagtctc aaagtgaact
     8761 tttgttcatg aggcactctt ttttgtatga aataaaatat attttaatca tttcaacaat
     8821 gaaaaagagg attatctctg ttttgagaca gcatctttcg ataataaaaa aagatgagct
     8881 tttgctcatc tcaaaggggt tagattattc ttactttggg gaaagtagaa taaaggtaaa
     8941 agttaactga tgtcaacttt gtcttttttg catatcacta ctgagcataa aaaaatcaga
     9001 aaaccatagc cttctgaaac aacttctaac atacattgca ttttataccc aatatgtaat
     9061 ttatgagtta attttaactc ttataatgaa aattgtcaat aatatatcaa aaaaatattt
     9121 tttaaattta tataattaaa t
//
LOCUS       DAEMHW010000151         9140 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEMHW010000151 DAEMHW010000000
VERSION     DAEMHW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.151"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            703..1080
                     /locus_tag="KPK67_002886"
     CDS             703..1080
                     /locus_tag="KPK67_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2459821.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVDKRYLARYIINFQSYYKIIIDNKEYYTFKKKVLKSSKSIKIMHSLT
                     TIHKNVSKEKLDLLDEYYRNLKE"
     gene            1185..1559
                     /locus_tag="KPK67_002887"
     CDS             1185..1559
                     /locus_tag="KPK67_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2459822.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELKEYFKNLK"
     gene            2171..2863
                     /locus_tag="KPK67_002888"
     CDS             2171..2863
                     /locus_tag="KPK67_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2459823.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            3059..5119
                     /locus_tag="KPK67_002889"
     CDS             3059..5119
                     /locus_tag="KPK67_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2459824.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDKAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(5325..5648)
                     /locus_tag="KPK67_002890"
     CDS             complement(5325..5648)
                     /locus_tag="KPK67_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBG2459825.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(5641..6342)
                     /locus_tag="KPK67_002891"
     CDS             complement(5641..6342)
                     /locus_tag="KPK67_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBG2459826.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(6620..8005)
                     /locus_tag="KPK67_002892"
     CDS             complement(6620..8005)
                     /locus_tag="KPK67_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG2459827.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSRYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(8113..>9140)
                     /locus_tag="KPK67_002893"
     CDS             complement(8113..>9140)
                     /locus_tag="KPK67_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG2459828.1"
                     /translation="IDNYIPYDQGITLDIFMYIWILGIIAISFYYLRTNFKFYNYIEN
                     LKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIISLTRCKIIIPEKKYNNK
                     ELRFIFKHELIHYKRKDNLFKLLISTICIIYWFNPILYLLKKYFHELCELSCDEIVID
                     KCTTSEIKEYAYIILDTIKYQKSLKYSSFVSQFLNKKNIILKKRLNSMFNQDKKKINL
                     GLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGTHIIDSNGNSIEPE
                     LIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAILYTRKRNFNIFKKTIPLYDKEGIKI
                     IGRFEIN"
BASE COUNT         3614 a         1001 c         1281 g         3244 t
ORIGIN      
        1 gacaaatata ctattatttt acaatttatg gaaatgattt aaaatgacat ttcttgttag
       61 aaaagatata agaaatggat taaaataaag aaaactacag tcatatcaat gtcaataata
      121 ttatttaaaa gcagatgaaa ataaaaagag gactcagaaa ataatgtcta tttaattata
      181 cttacttacc aataccataa cttgatataa acaagattat actacatttt ttttatgctc
      241 tagtataaaa tcatataact gtgtatctat aaaatcaaaa taattatatg aactatcagt
      301 ataataagtc aaaatatgta tacatgaata catattttaa aagatatata ttattacata
      361 cctaaaaaac tttcataaaa acaaaaataa aatatgcttt tagtaacttg ttaacataac
      421 ttataattta ttttataagc attagcctat atagctttgt gctactaaat ttctttgatt
      481 ctagcgattt agtgctattc tacaactaaa ataatacaat agagctattt attgtttggt
      541 agcattgttt gcaaattaaa caaatttata tatatgaata ttattttacg tttaaaaaac
      601 agactaaata tgtaaaatat ttggtaaaaa ttacctctag tttttgtctt aaaaagctcc
      661 tattattaat ttaagttaga agaattaagg aagtgacata aattgaaaga agaaatatta
      721 atagaaaaat tattaagagc agaattaata gtaatggagt atttatggaa aaaagacact
      781 ctaatgccta aaaaagaaat tgtaaaagaa ataaaacaga tttatggatg gcataaaagt
      841 acaataaaaa tactactaaa aaggctagtt gataaaaggt atttggctag atatattata
      901 aattttcaat cttattataa aataataata gataataaag aatattatac ttttaagaaa
      961 aaggtactaa aatctagtaa aagcataaaa ataatgcatt cacttaccac tatacataaa
     1021 aatgtaagta aagagaagtt agacctatta gatgagtatt atagaaactt aaaagaatga
     1081 tatagtaaaa atcgaatgta attatttacc ttaattcctt tgtttgtaaa actttattga
     1141 tatagaattt gacatataca taaattggta aagggatgta tactatgaaa attagtaaat
     1201 taccagaagc ggaattaaaa gttatgagat atatttgtga agctaacaag gtattaactt
     1261 ctagggaatt agttgctgca atggaagaaa aatatgagtg gaatgaatca actacattta
     1321 cagttttgaa aaggttagag cgaagggagt ttttaagcac agagaaaata ggcaaactta
     1381 cacattacaa tatactagta aaagagaaaa aatatctaag gtttgaaaca aaggaatttt
     1441 tgaagaacat acataaaaat tcaatttcaa gtttaatgtc agcgttacat ggtgaagacg
     1501 acgaagtgga tgaagataaa ttaagtgaac tcaaagaata ttttaagaat ttgaaataag
     1561 aaaatgacaa attgatacat aataaataaa atgaaggtat gaaattataa ctttttctac
     1621 cttcattttg tttattattc tgaacatgtt aagaaaatga aataagtttg tattttagtt
     1681 atataaattt taatagaata tgtacagtat atagtagata gaatgtataa aaatagaatt
     1741 taaataagta gctattattg cagtgaaatg aatgtttcta agtatatttg ggtggaagaa
     1801 tggtggaaga aaattagtga aatgtaattc ttccaccatt cttccaccca aaaaataaaa
     1861 actgttcaat aagaattaat atacatcaat tcagctgaat ccaatcaaaa tacttaaata
     1921 aaagtaatct gaaaacaaca taattcaaat caatttattc aaataaacct caatttatct
     1981 taaatcttaa gaaatattta agaattagga taatattttc ttaagattta tattttatta
     2041 tatagttata agggaaagtt acaaaaatgt aagccaaagt aatataacaa tttagtcatt
     2101 gttgagaaag ggagtgaaag atattgtata atatagataa gtattattat acaatagagg
     2161 aagtgaaaat atgtacaata tattagtagt agacgatgat aaagaaattg ttgattcgat
     2221 agaaatatat cttaaatcag aaggatttaa aatatataaa gcatatgatg gaatgaaagc
     2281 tttggaaata ataacagaga atgacattca tctaatacta atggatataa tgatgcctaa
     2341 attagatgga atcaaagcaa ctgtaaaaat acgagaagaa agaaatattc caataatttt
     2401 aatttcagca aaaagtgaag atacagataa aataatagga ttaaatattg gagctgatga
     2461 ttatataaca aagccattta atctattaga gcttatagct agggttaaat caaacttaag
     2521 acgatatgta actttaggta attatgaaga tacatgtaaa gaaattttaa aaagtggagc
     2581 tttggagtta aatacactaa caaaagaagt taaaattgat ggtgaaaata taaaaatgac
     2641 tccaattgag tataaaataa ttgaacttct tttagaaaat aaaggacgag ttttctcaat
     2701 agatgagata tacgaaaaag tatggaatga ggaaagtttt aattcagaaa atacagtagc
     2761 agtacatatc agacgaataa gagaaaagat tgaaataaat ccaaaagaac cgagattttt
     2821 aaaggtggtg tggggagttg gttataagat tgaaaaattt tagtggaaaa attataaaag
     2881 aaaagagttt aatatttaat tgcattttga ttttagtcat ttttacattt acacaaatgt
     2941 taggaatttt aatatgtttt aattttttct ctgagagttt aatttactct atggggatta
     3001 tgcttttctt aaactttatt ttatataaaa tactgacaat gtagatgagg tgggcaactt
     3061 ggagaaatat agagtattaa agttagtttc attaatcata atatctatct catttccatt
     3121 aactatattg ttggtgacaa taggacttgc tgagcctaaa tacaatatac aaaaaacttc
     3181 tacaaagagt gggatttttg atatatctga tgatttttat gatagtaata tatttgacga
     3241 aggaacaatg gcaagtctat tacccaagtt taagctatct gtaggtggta aattaactag
     3301 taaagaaaaa ggtaaattcc aacaagaagg taaaagtata gatttaagta gtagtgattc
     3361 tgagtatgat aaaatcatga ttaatgaaca agcttctgaa gaatatttaa gttatctaaa
     3421 aaatataaaa gtgtatgtta ttaatacata cacaggagta ttttatacaa atacgtcata
     3481 tgctagttta tatgatttta aacaaaaaac taagagctat tgtaatgtag agataataaa
     3541 taagatagga aagagctcat atattaaaac aataaatgga gaaaaatttg aactgaataa
     3601 tctgagtctg gaactaaatg aagtagatga aggttttgag gcatatgtat cttttccaga
     3661 agagccaact atagaagatg gaatagtata tactaatttt caaatattta aacaagctac
     3721 agaaaaagta agattatttt ttgtagctag tttaagttta gcaattatat gtttattatc
     3781 tataatttta tatatcaaaa taaaaggtga agttttaaaa agaaataatg tatttttact
     3841 aatatacagt aagataccta tggaatttaa tgtaatatta gtatctattt caattgcaat
     3901 tttaactata tatgctcttg ctgtatctta tcatattgat gtaattgcat tagtttcttt
     3961 atctttactg atagtatcaa ataatatatt ttttataaat gtgcaattga gttatttaga
     4021 aaataaaatg tgtatattta agacaagtat tatagtaaga gtgattttat ggttaataaa
     4081 aactgtaagg gaggtcagta acgctataaa gaaagtatct ttagcaagga aagttattgt
     4141 tatagtgata atatctttag ctttaatcat ggtaattagc actcgaagta taggtatggg
     4201 attactgttt tctatatgta tttttttagg atttacaata tatataacta aaagactttc
     4261 atatcttagt tacgtaatag aaggtacaga aagaataaaa aagggagaat tagattataa
     4321 gataaaaatt actgggaatg ataattttac gtctttagct gaaaatataa ataatattgg
     4381 agaaggtctt gataaagcta tagaagagca agtaaaaagt gaaagaatga agtctgacct
     4441 tataacaaat gtatctcatg atttgaaaac tccacttact tctataataa attatgtaga
     4501 tttaattaaa aaagaagaat caatacaacc agagtatata aatgattata taaatgtact
     4561 tgagtcaaag tcaaaaagac taaaattatt gatagaagat ttatttgaag cgagtagagt
     4621 aagtagtggc aatatagaat tacaaattag caagattgat ttagtgcaac tattaagaca
     4681 aagtataggt gaacttgaag aaaaattatc taaaaataat ttatatttaa aattaaatgt
     4741 tccaaatgat aaggtgtata tatgggcaga tggaagaaga atgtatagag tatttgaaaa
     4801 tcttttatct aatatagcaa aatattcctt agaagaaact agagtctata ttgatgtata
     4861 tgactatggt gaaaatgtaa aggtaactat gaaaaatata tcttcttatg aattaaattt
     4921 tgacccaagt gagattatgg aaagatttaa aagagctgat gaatcaagga atactgaagg
     4981 aagtggatta gggcttgcta ttgcaagaga tttagtagct ttacaaggtg gaaaatttta
     5041 tatagaaata gatggagatt tatttaaagc aaatttagag tttcaaaaat atgaagaaga
     5101 aataacaaat atggaatgag atagtattta agtatatata ttttttttac tgtcgaaagc
     5161 ttttattata atatattaga atatttacag tagagaatga agagtaaaaa cagagtaact
     5221 ttaagtcact ctgtttttta tttaaaatca ataatataac aacttaaatt aaaattgtac
     5281 acatagtttt aaaataactt atacaataaa acattatcca aatatcacaa gataattcca
     5341 aataaatata tagatatcac tcctgtaata atagaaatac caatagattt aaacttatat
     5401 gcaacaagaa gtacaatcat aaaagatata attttagggt tagctataga taaatataga
     5461 ctatctccat taaaaaatac atctttagca gttaaggaag taagtattgc tacaggtata
     5521 aatttcatga tttttttcaa aaaatcaggc atctcttttt ttgaaaaaat taacatagga
     5581 agcactcttg gtagatatgt aacaatgtac attccaatta taataaaaaa tatataatta
     5641 ttattcataa gattcctcct ttttacagta agattcttct ataaagaaac caattaaaga
     5701 tgctaaaatt gatgcaacta ttattactaa attgcttttg aataatgcat ataaaataac
     5761 agaaataatg attgatgata taaatactac aatataaatc ttatttttta tttgagaaac
     5821 tattaaagct ataaacatag aagtcaatat gaagccagaa atagaactgt ctatagatat
     5881 actttctcct aaaaatgctc caataaaatt agctaaaact gatgaaatta agcagaagaa
     5941 attaagataa atagcatttc tatcagacca atttccagca gtaaattttt cataattaac
     6001 tgcaaaagtt tcatctgtta tagtatgaca aaatggcaat ataaattttg gacttttatt
     6061 ttttatgtac atgttcaaat tagaactcat tagaaaatgt cttaagttaa ctataaatgt
     6121 agtcaaaaca atagatgtta cacttgcacc agtagaaatc attgaagctg caatgaattg
     6181 agaacttcct ccaaatacta aagttaccat tagtgctatt tgaaaaggtg aaagaccaga
     6241 tttttgtaat agtattcctc ctactatacc tataggtata taacctaaga atataggaat
     6301 tgaagcaata aagctttctt ttaattgttg agttctttcc acaataaatc actccttttg
     6361 tattaaatgt attaaagtat tagtaaaaca gaaaaataga ttaattgtat aattgcaatt
     6421 ataattaaaa ataaatttgg attaatatat aaaatactat acaacaaata gataatctta
     6481 aacggttatt aaattttatc tatttgttat tatgatgtaa taatacatat taatctctat
     6541 aagttaatta ctatctggtg taattttatt ttaacaacaa aaaaaaatac acacaattaa
     6601 aatattgtgt gcaatacaat tattttttct cttctaatag tctttttaat acgctcagac
     6661 cagctttaag ttcttctgtg ctttctgtag cacatacagc tattcttatt gcagaaattg
     6721 gtttttcttt tccaacagca aaacgttcag aagcatagac ctgtacacct tctttaagtg
     6781 cttgtatctc aaatttttct gccattattc catcaggtag aatcaaccat ctaaatatac
     6841 attcttcatt tcccaaaaga ttatatctag ataaatattg attaataatc ttgtttcttc
     6901 ttcttgcaga tttacggcgt gcattaatta atttatcggc ttcacctgat gtaatcatgc
     6961 gataagcaat ttctgttaaa aaataagatt gagatagatt aatgttgtat aatgcgcttg
     7021 ataatgcctc tcgatactgt ttaggtgtag agatatatgc tagccgtaaa ctaggtgcaa
     7081 taatttttga taaactcgta atataaattg tttgattggg cagataggaa gccactggat
     7141 ttaaatgtgt ttcattaagt aaactatgaa tagcatcctc aataacaatt aaattgtatt
     7201 tagatgcaat atttgcaatc atttttcgtc cattttgtga cattatatgt gtagttggat
     7261 tttgataatc tggtattatg tatagacctt taatattttc atttttacaa gcatataata
     7321 gtccttcttc actaatctca ttatcttctt gtttaattgg aattagttga atcccaagca
     7381 ttttagctgc tgtctttatt ccaggataag ttaatgggtc tacacctatt ctatctccat
     7441 gttgaaacaa accagctaaa atagcaacaa ttgcattttg tccaccactt gcaggaagta
     7501 agctatctga tgtagtttca agcccagctt tataaattaa tttggcaata gcttcttttt
     7561 gccatttagc accatctctt tgtccatatt ggaatatggt tttaaaatct atttccttaa
     7621 ttatgtgttt aaacaaagta ttcatttctt ctagcgtgaa atctggattc attgtgccca
     7681 tttcaataaa tgtaatttta ttattactgg acatcaagaa tagatttgaa cgcgtatcat
     7741 aagatacaaa agttccactt cctgtaactc cacttattag accttttttt tcacaaattt
     7801 taaaagctct agagatagta ctcacattaa catctaagaa atctgccaat tcacgttggg
     7861 gtggtaattt agtacctggt aataaaaatc cattgttgat atcttgttct agttgttctg
     7921 caagcgcctt atataatatt tgcccttttg aaggtctctt tggtttccag ttcataggat
     7981 aattttcaaa agaattaatt ggcataagtt ttcacctcac attattaatt tgattttttc
     8041 tttatctaaa acaataaatt ctaaagatac aaataaaaac tgataggaaa taattctatc
     8101 agtttttgtc ttttaattga tttcaaatct tcctataatt tttatacctt ctttatcata
     8161 aagagggatt gtttttttaa atatattaaa atttcttttt cttgtatata aaatagcttc
     8221 ttctggtgtc tttggttgat tatcacaatc gtacaaatct ctttttttta cgtagccgac
     8281 ttttttacct ctacctattg tttgtataag ttctggttca atggaatttc catttgagtc
     8341 tattatatgt gtaccatagg tttctccatt tttattataa ttaatcttac caatagtata
     8401 ttctacatct tcattttgtt taaaattgga gttgatagaa aatattgacc ctattattat
     8461 aaaggttaat aaaaacttaa gacctaaatt aattttcttt ttatcttgat taaacataga
     8521 atttaacctc ttttttaaaa taatattttt tttatttaga aactgagata cgaatgatga
     8581 atacttcaaa gatttttgat actttattgt atctaatatt atatatgcat actctttaat
     8641 ttcactcgtg gtacatttgt caatcacaat ttcatcacaa gataattcac ataattcatg
     8701 aaaatatttt tttaataagt ataaaattgg attgaaccaa tatatgatac aaattgtact
     8761 aattagaagc ttaaataagt tatcttttct tttataatga attaattcat gtttaaagat
     8821 gaatcttaac tctttattat tgtacttttt ttctggaatt attattttac agcgtgttaa
     8881 acttataatg gcaggagaat aaataccttt aaccttatat atctgaagtt ttttatttat
     8941 acttagttct tttgcttgtt catctaagat actagtaata tctatttcta aaatttcttt
     9001 ttttaaattt tctatgtaat tataaaattt aaaatttgtt cttaaatagt aaaaagagat
     9061 tgctataatt cctaatatcc agatgtacat aaatatatct aaagtaatgc cctggtcata
     9121 tggtatataa ttatcgatat
//
LOCUS       DAEMHW010000152         9132 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEMHW010000152 DAEMHW010000000
VERSION     DAEMHW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.152"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..521
                     /locus_tag="KPK67_002894"
     CDS             <1..521
                     /locus_tag="KPK67_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2459829.1"
                     /translation="CNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLD
                     DYITKPFSLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYK
                     LLSYLIENKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDNPSNPKYIKT
                     AYGMGYLWNEVE"
     gene            523..1431
                     /locus_tag="KPK67_002895"
     CDS             523..1431
                     /locus_tag="KPK67_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2459830.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            1508..2194
                     /locus_tag="KPK67_002896"
     CDS             1508..2194
                     /locus_tag="KPK67_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459831.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            2191..4746
                     /locus_tag="KPK67_002897"
     CDS             2191..4746
                     /locus_tag="KPK67_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2459832.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(4940..6139)
                     /locus_tag="KPK67_002898"
     CDS             complement(4940..6139)
                     /locus_tag="KPK67_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBG2459833.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            6258..7145
                     /locus_tag="KPK67_002899"
     CDS             6258..7145
                     /locus_tag="KPK67_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG2459834.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            7335..7892
                     /locus_tag="KPK67_002900"
     CDS             7335..7892
                     /locus_tag="KPK67_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2459835.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(8131..8991)
                     /locus_tag="KPK67_002901"
     CDS             complement(8131..8991)
                     /locus_tag="KPK67_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG2459836.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
BASE COUNT         3551 a         1127 c         1353 g         3101 t
ORIGIN      
        1 actgtaacct tccagatgga gatggatttg atgtatgtca aataataagg gaaaagtcag
       61 atataccaat tataatgctt actgcaagag actcagaaat tgatgaagta aaaggattag
      121 aaatagggct tgatgattat attacaaaac cattttcttt atctgtatta aaggcaagag
      181 tcaaagtagc aataagaaaa aaatcgaata ataaagtaat atattccaat ggaataaaat
      241 tggaccaaaa gttactaaaa gtatataaaa ataaagaatg tttggaactt agctctgtag
      301 aatataagct tctaagttat ctaattgaaa ataaaggtca aatattatta aaggaacaaa
      361 ttttacacca tatttgggac agcgaagaaa attatgttga tgataatatt gtatctgtaa
      421 atataagaag acttagagta aaagtagagg ataacccatc taatccaaaa tatataaaaa
      481 cagcttatgg aatgggttat ttatggaatg aggtagaata atatgtttat attagccttt
      541 ataatatgta cagttgttat atttatagta tctactaaaa taaatcaaag aagatatgat
      601 gaattaaatt tgatgttaaa tcaaatattg gaaggtaaag aagttactta tccagatacg
      661 aaggatacaa gagcgtcaaa aatttctcac caagtaaaaa aaattaagga tatgatagaa
      721 atagaggtag aacagtcaaa gtcagaaaaa gaagctatta aaggtttaat atcaaatatg
      781 tcacatcaat taaaaactcc actttcaaat ataaccattt attgtgaact tctggagaat
      841 ataaatattt ccacattaca gaagaaggag tttttacaaa agatgaaaaa tgaaactttt
      901 aagattgact ggttgttaca atccttattt aaaatgacta aattagagga tggagttata
      961 gaatttgaag tagaagaact actaattaaa gatactctta ttcaaagtat cagtactatc
     1021 tttaataaag cagaagctaa aaatataagg ataaatctcg aaccattttc agacataaag
     1081 ttagttcata ataaaaagtg gactatagaa gcaattgtaa atgttttaga aaatgcaata
     1141 aaatactcac catcagatag cacgattact atttctgtaa taaaaatgga actatatacg
     1201 aagattacta ttaaagatga aggaataggt attgatagta gagaactaaa tgatattttt
     1261 aaaagatttt acagaagtaa aaatgtggaa aaccaaaatg gcacagggat tggtctatat
     1321 cttacaagat taattttaga aaaagaaaat ggaaacatta tagtagaatc taaactagga
     1381 agtggatgtt gttttagtat cttcttacag aattgtaaga gtttaaacta attttgtagg
     1441 atgagtgtaa ttatcgtcac ttacaatatt ttatagaaca tatatctgta ttgaaaggag
     1501 aaattaaatg agtatattag tgacgaataa tttagtaaaa cactatggaa aaggtgagac
     1561 taaagtaaaa gccttaaatg gagtaagtat agaaattgaa cgagatacat ttactgctat
     1621 agttggaact tctggttctg gaaaaagcac attgttaaat attataggtg gattagataa
     1681 tccaacttca ggtgatgtaa taattaaagg aaagaacata tctaaaatag gaaaaaaaga
     1741 tttgacagta tttagaagaa gaaatatagg atttatattt caaaactaca gtttaatgcc
     1801 cgtattaaac acttatgata atatagctct tcctgttaca ttagataaag gaaaccatat
     1861 agaccatgaa tatattgaaa tgctgatgaa gacattggga atttgggata agcgtttaaa
     1921 atttccaagc gaactatctg gtggtcaaca acagagggta gcaattgcta gagctttggc
     1981 aaataaacct gcgcttatat tagcagatga accaacagga aatctcgata gtaaaactac
     2041 aatggaagtg gtatgtttat taaaagaaag tagtgcaaaa ttccatcaaa cgatacttat
     2101 ggtaacacac aatgaaaata tagcacaagt atgcgactca attatccata ttgaagatgg
     2161 agttgtggtg aatactggtg gtgaagtact atgatgtctc tagtaaaatt ttcaattcaa
     2221 tatataaagc attataaaaa acaatctata tctatagttt taagcatagt actatcagta
     2281 gcattattga cgggaattgg ttctttagtt catagtgcag acaaaagtag aattgaaaag
     2341 ataagggaag atagtggaga ttatcattat ttctataaag tagataagga acaacttaat
     2401 aaaattaaaa aaaataaaaa atcaaaggaa tacactgtag atagacttgg cgttacaaaa
     2461 cctaaaaatc agatagatga accttttatt atagaatttt taaatgctga ttcttcatat
     2521 ttagacatga caggtagaaa attattaaaa ggaactcttc ctagtaaaga tggagagata
     2581 gtacttgaca catattctct aaacaattta aatgtaaaca aagaaattgg aacagaaatc
     2641 aatttagatg gtaaaatcta taagttatgt ggtattttat caagtctttc aaacccagaa
     2701 actgctctgg agggatttgt aaacgattat tttcagccta aagaaaagga ttcttatata
     2761 gtttatgtaa aatatgatga aaataaaaat attaaaaagc aaaattataa atttatgaag
     2821 aaattcaata tatcaaaaga aaaaacttat ataaactggc ctctttcagt tgaatttgga
     2881 gtaaaaccac caaaaaatat agaaaaatat aatatatttg agttcttaaa aaatttagaa
     2941 ctaaatacaa atgcaattat attgcttgtt ggagtgtttg gtgcagcagc agtatatagt
     3001 attttccatg tttcaatact tcagagaata tcacaatatg gggttttaga agtattaggc
     3061 gctaataata aacaactttt atttctacta tttttagagt tatttttact ttttattgtt
     3121 ggatttccta taggttgctt cttaggtatt ggagtagcgt ctacaatata tgaacaattt
     3181 ccacatatat ttttgagtag tgatattgtt ccaggagcat tttttataag tgaaaaatct
     3241 atttatttag gctttttatt tatcttattg ttgttaattt taattgctgt acgtgttgta
     3301 catcagttga ataaatactc tagtatagaa tctatgaaaa actttaagat tactaataag
     3361 aaaaagagaa tattagaaag tgtaaaacac tctaacatga caaaagtatt atctcataaa
     3421 tacatgactc aaaaaaaggg aatgtttatt ggaattttgt tttcattatc acttggtgga
     3481 attatatttc tatgttctag ttactctata cagcttacta aaaataacaa tgaacttaca
     3541 atgaaagctg atgatggttt aaattctgac taccaaataa ctatgcaaac aacagatttt
     3601 gatgtaggta taccaaaaga taaaatttca agattaaaca atgttgaggg aatatcaaaa
     3661 gtctatccag taagatattt atttggagct atgcaaatta aagaagaaca actattttgg
     3721 aaaaatttct ttaaacctct tgaaaaagat tacaggataa agactttttt tgatggtatt
     3781 tgtacaaagc aaaatgaagg tggctatctt ttaaaaacaa atatttatgg ttataacaat
     3841 ggtatgttag aaggattaaa ttcttatatt atagatgggg agattaatac ccatgatatg
     3901 gttaaaaata ataaggttat agttagacta cctatggatg gaactgggat gtatgatgca
     3961 gttgatataa atccaggaga tacaataaaa gttaaagtgc caaagacaat gaaaccaaca
     4021 gatgaaattg ttaaatttaa agaagaaaat aagaatgatt atacaacaaa agaatttgta
     4081 gttgctgcta ctgtaaaacg tgtgatggca aataatatat atttcattgg tgattatggt
     4141 atggatatag ttatgacgaa tgggcaaatg aaaaataact ttgatatcga aaattataat
     4201 tcagtaagta taaaaaagac agaagatagt aacagtgagg aattgtctga gaacataaaa
     4261 agtgttgtaa gtgatgtaaa aagatgtatc gtaacagatt ataccattgc tattgaaaaa
     4321 aataatacct acttaaatca aaaactctta tttatatatg gtattgtatt tgtacttctt
     4381 gcaataagtt tgttccatat aatcaataca gtaagctact taattttttc aagaagacat
     4441 gagtttggaa ttttaagagc tatgggtatt acggataata agtttttact tatgatgata
     4501 agagaaggtt ttttatatgg attctatgca agtattataa tggtaattgg aagtgttatt
     4561 ggacaattta tgatttattt tatggtaaaa cgtgtatatt tatatataaa tccaattctt
     4621 aaaataaata cacctctata cataggtatg attattttaa atatcactat tagtatagtt
     4681 gctgtaatta ttccagtaag acaaattttg aagagtgata ttatttcaga aataaataag
     4741 aattagagtt atataaattt ttaagattat ataactttct gacaatcttg tttaaattat
     4801 ttaaatatat cttatctgtg aacattaaat aaacctctta atagatataa ctcttgaaaa
     4861 gtcatatcta ttaagaggtt ttaataaatt ttcatgttaa tatatataat ccaaaaaaat
     4921 taaatcttat taaaacatac tattttgtat agttatcgaa atatccttgt atgtaaataa
     4981 taggagtacc cttatctcca cttccagatg ttaaatctgc taaagaacct ataaggtctg
     5041 ttaatcttct tggagtagta ccttgagaaa ccatgtttcc tgttaaatca tcagatttat
     5101 tatctttttc aactatgtat tttgaaattg cttcttttaa ctcatcacca gataagtcag
     5161 caaaatcatt atctgctaag tattttaatt ttacttcgtt tggagttccc tctagccctt
     5221 ttgtataagc aggagatact actgggtcag ctaattccca tattttacct acagggtctt
     5281 taaaagctcc atctccataa accattactt ctacatcttt ccctgttatt tcttttatat
     5341 taccttggat tttgtttaca acttcatcac aatttatagg gaataatttt actgtttctt
     5401 ctgtagcttt gtttgaacct aaaagtccat actggtcatt gtaaccacta ccatctacac
     5461 ttgctgtcat tatatcatca agtgaatata cttttttagc tccattttgt cttaaaattc
     5521 tctttgttct ttgtcttgta tgtatatcac aagttaaaat acttgttgta tagttaagaa
     5581 tagtttttgg attgtttgca aatactattt ctacctctgc accacaatcc ttgattaaat
     5641 tcttataata atcaacataa tctacaccag taaatgtatg cttgttatat ccaaatagtt
     5701 ctctgtattt ttcttctgtt aaaacatctg accaaggatt tattccctta tcatcaagtt
     5761 catcaatact tattaagtga tttccaactt catcagaagg gtagcttaac attaatacga
     5821 cttttctaca tccttttgca attcctttta aacatatagc aaaacgattt ctacttaaaa
     5881 ttgggaatat aaccccaaca gtatcatctc caaatttatc ttttacatct tttgcaatat
     5941 tgtcaacgtg agcataattt ccctgtgctc tagctacaac agcttctgta acggcaatta
     6001 catctttgtc cctaacttca aagttttcac tttttgacgc atttaaaaca gaatcaacaa
     6061 ctattttaac taagtcatct ccttgtctta taataggtgc tctaacccct cttgaaacag
     6121 ttccaactaa tctatccatt atctaaaatc ctcctaaaga attaatattg tcatattagt
     6181 accatgtata ttataattca aatataatta taagtaaaat aaataatact aatatttgac
     6241 ataaggagta attatatatg aatataaaac ttgaacttta taaggttttt aatgcagttg
     6301 taaataataa gagtttttct cttgctgcta aggaattgtt tatgtcacag ccagctgtaa
     6361 gccagtcaat aaagcaatta gaagaacaat tagatacttt attattttat agaaacaata
     6421 agggagtaaa attaactcca gaggggaaaa ttctaagtga acatgtcact actgcattaa
     6481 agttaatatc atcaggagaa gatagaataa ataaatttaa aaaattagaa tatggttctc
     6541 tgaaaatagg agtaggagat acagctgcac gttttttctt gttaaaatat ttagaaattt
     6601 ttcataagaa gtatccacat atacatgtat ctacaataaa tagaacgagt agagaactta
     6661 tatctttact caaagatggg aatatagata tagcaattat aaacatgcca atagaagatg
     6721 atacactaaa tattgttgag tgtattgaaa tacatgatat atttgtatgt gcaaatgatt
     6781 acatagaata caaaggtagg aaaatatcat tggaagaatt aaacacattg cctttaataa
     6841 tgctagaaaa taaagcaaat tcaagacttt atgtaaatga atatttttta tcaaaaggta
     6901 taaaactaaa cccggatata gaattgggtt ctcatgaatt acttttagaa tttgcgtata
     6961 taaatcttgg agtgtcttgt gttatagaag aatttagcat agattatttg gaaaatgaga
     7021 aattgtttaa attagatata aaagagccaa tacctaagag aaatataggt tactgccatt
     7081 taaaagatat atctttatcc ttagcaacca aagaattttt aagcatgata tctaataata
     7141 tataatttag aatacatatt tcacaagtaa tttaatataa aaatagagat aaattcgtaa
     7201 acttgaaaga ttcatatagt aattagtttt tattaaaaat aaaaataagg ttaatttgag
     7261 aacatttata taattaatat cgtattttaa atatgaacag tatatttaga ttaatgtata
     7321 aagggggatt agaaatgtct gattctgaat atcctcaaat gaaaacctta gaaactagaa
     7381 gttgtatact aagacctgca tcattagatg atgttgaaga tttttttgac tgttataaaa
     7441 ataaaatagt agtaaaacat ttaccattta gtgagcataa atctttagaa gatacaagaa
     7501 agtttataaa atcatttttt ctaaatccat ataaaaaagg gaaaattggc cactttgcta
     7561 tagtatataa aagagacaat aaagttattg gaaatatggg gtttaataat ataaatccta
     7621 aagcattaga agcagaaata ggtatatgta taaatccaag ctattgggga catgattttg
     7681 ctactgagat aacaaaaaga gtaatacagt atggatttag agaactaaac ttaaataaaa
     7741 taattgcaat aacctatgaa gaaaaccaaa attctacaaa atcattagat ttattaggat
     7801 ttaaatgtac aggaaagtat atcaaaaaaa ttcatactgg aaatactgta aaaaatgttc
     7861 cttgttatca atatgaattg aaaaaacaat agatataatc atttaatatc tatgctggtt
     7921 atttagtaat gattgttttc taaataaaat ttcaataaag tggttgcctc aaaatataga
     7981 taatcctgtt tttattgtta aaatactgaa aatgcatttg attttataca aaaaagagtg
     8041 tctcatgaac taaaaagtct atgagacact ctttttgaat aaatcgtatt ttaattaaaa
     8101 tagtatatat acttaaaata ttaattttta ttagttctct aaatttttaa atccttctcc
     8161 aagaacctca tgtacatctg taacaataac aaatgcagta gggtcagttc ttttaactaa
     8221 tctctttaga taaagttctt gttttttaga aactacgact aatagaactt ctttattttc
     8281 tctagtgtat ccacctttac catcaagaat agtaagacct ctatcaagtt cttcagttat
     8341 agcttgtctt aaactttcag gctcatcaga gataataaaa aatgcttttg aatggtcaaa
     8401 tccttccatt atcatatctg ctatttttat taatacacaa agtgctatac cagagtaaag
     8461 agcagtttct aaactacgat ttactatacc agaagatata acaaccattc catctaggca
     8521 tgacatgaat tttgtagctt ttattcctgg gaatttctta ctaagtatga gtgcaattaa
     8581 gtctgttcct ccagtagaag catctgattt aaacattatt cctaaaccta taccaactag
     8641 gattcctcct gcaatagaag acaataataa gtcattagta aatcttagct ttgataatgg
     8701 gtctgttaat tgaactatag ctgaaaatac aactgttcca aataaagttt ttaatgaatt
     8761 ttttgtaccc ataattctaa aagcaagtat tactaatggg acacctataa ttaacatgat
     8821 agctgatact ggaagtcctg taaccttgtt tagtactaat gatagaccac taagtcctcc
     8881 aggagcaatt gtatgtggtt ttaaaaacat atttatacca atactcattg atatacaacc
     8941 aaaaaatagt ccaagcattt caagtaaaat tggcgacttt tgttttttca tataaacacc
     9001 tccatgaact tgtcacaatt taatattata acatatttga ccagtttata ttatataaaa
     9061 tttcttgata taagcaattt caagtatttc attattctta atatttttga atttttatat
     9121 agtgttttca ta
//
LOCUS       DAEMHW010000153         9115 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEMHW010000153 DAEMHW010000000
VERSION     DAEMHW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9115
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.153"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..826
                     /locus_tag="KPK67_002902"
     CDS             <1..826
                     /locus_tag="KPK67_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2459837.1"
                     /translation="ILDFPDMSADGSYTMGASIVAFSLTNGISPVVATLMAILCGCTA
                     GLVTGILHIKFKISNLLSGILVMGMLYSINLRIMGKSNIPLFSFKHLFNGEISPIVLA
                     LAFVFICKVLLDLFLKTGLGYTLKGVGDNSQMIKSLGINIGSIKILGLMISNGLIALS
                     GSLMAQFLGFSDVNMGIGTLVLGIASIIIGITLFKKFTFIKDTTAIIVGSFIYQFTIY
                     FAMSLGMLSTDLKLITAIVIIAFLATGNLNISLKKNKCKASTKNKSEKRGVVDVTN"
     gene            813..1568
                     /locus_tag="KPK67_002903"
     CDS             813..1568
                     /locus_tag="KPK67_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2459838.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            1699..2721
                     /locus_tag="KPK67_002904"
     CDS             1699..2721
                     /locus_tag="KPK67_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2459839.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(2885..3442)
                     /locus_tag="KPK67_002905"
     CDS             complement(2885..3442)
                     /locus_tag="KPK67_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG2459840.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(3448..4524)
                     /locus_tag="KPK67_002906"
     CDS             complement(3448..4524)
                     /locus_tag="KPK67_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBG2459841.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(4525..5208)
                     /gene="modB"
                     /locus_tag="KPK67_002907"
     CDS             complement(4525..5208)
                     /gene="modB"
                     /locus_tag="KPK67_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBG2459842.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGILVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(5357..6169)
                     /gene="modA"
                     /locus_tag="KPK67_002908"
     CDS             complement(5357..6169)
                     /gene="modA"
                     /locus_tag="KPK67_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2459843.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            6362..7291
                     /locus_tag="KPK67_002909"
     CDS             6362..7291
                     /locus_tag="KPK67_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2459844.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     IIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(7452..7901)
                     /locus_tag="KPK67_002910"
     CDS             complement(7452..7901)
                     /locus_tag="KPK67_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459845.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(8104..9084)
                     /locus_tag="KPK67_002911"
     CDS             complement(8104..9084)
                     /locus_tag="KPK67_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2459846.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         3135 a         1478 c         1131 g         3371 t
ORIGIN      
        1 aatcttagat tttccagata tgtctgctga tggtagctat acaatgggtg cttctattgt
       61 agcattttct cttactaatg gtatttctcc tgtagtagca actttaatgg ctatcttatg
      121 tggttgtact gctggtcttg ttacagggat tcttcacatt aagtttaaga tatctaattt
      181 actttctggt attttagtta tgggaatgct atactctatc aatttaagaa taatgggaaa
      241 atcaaatatt cctctattta gctttaaaca cttattcaat ggggaaatat ctcctatagt
      301 tttagcctta gcttttgtat tcatctgtaa agtcctttta gatttattcc taaaaacagg
      361 tcttggatac actttaaaag gtgttggtga taactctcaa atgattaaat ctctaggtat
      421 aaatataggt tctataaaaa tactaggact tatgatttca aatggactta tagctttatc
      481 tggtagtctt atggctcagt tcttgggatt ctcagatgta aatatgggta taggaacttt
      541 agttcttgga attgcctcta tcataattgg tattacatta tttaagaaat ttacttttat
      601 caaagatact actgcaataa ttgttggctc ttttatatac caattcacta tatactttgc
      661 aatgagttta ggaatgttat caactgactt aaaactaata actgccatag ttataatagc
      721 tttcctagct actggtaact taaatatttc actaaaaaaa aacaaatgca aagctagtac
      781 caaaaataaa tcagaaaaaa gaggtgttgt agatgttaca aattaaaaat ctttcaaaga
      841 gtttccctaa tccatatggt gaacctaata ctatctttga aaatttatca atcgatatag
      901 aagatggtga atttgtaagt atcataggta gtaatggaac tggtaaatct actttattaa
      961 atataatatc cgggcttata aaagaatctt ctggtcaagt attattaaat aaacataact
     1021 tgtctaattt agctgaacac agaagaactc aaatagtaag tagagttttt caagacccaa
     1081 gtcttggaac ttgcccttct atgactgtta gagaaaactt gtctttagct cttaacaaag
     1141 gaaaactatt aaatctaaga aaatgtcttc gttacaaaag agatttctta gaaaatttat
     1201 tggaaggtgt atctcttgat ttaaaaaaat accttgatgt tcaagtaaaa tttttatcag
     1261 gaggtcaaag acaatcatta tcactaatta tgtcgtgctt aacaaatcca tcagttttat
     1321 tacttgatga acatacagca gcacttgacc caaaaacatc aaatgaagta attgaattga
     1381 ctaataagat tgtaagggaa aaaaatataa ctacacttat ggttactcac aacttaaaac
     1441 acgctcttca ttatggtgat agattgataa tgcttcataa gggtgaagtt gtattagatg
     1501 taaaaggtaa agaaaaagaa caattaacag ttgaagaaat cttagaaaaa tttgaatatg
     1561 cagtttaaaa taatatatct attgtattaa actcggcttt aaaataaagt taaaattaca
     1621 ataattatgg ttactttgta ataattcata tgaatatatg aacatatata ataataaatt
     1681 tttgaggggg atataaaaat gattaataaa aaaagattgg caagtttgat tttagctggt
     1741 gctcttagta tatctatgct tacagggtgt tcacaaggag gagactctgg taattcaaaa
     1801 caagaaagta attctaaagg taaagaagtt aaaaaaattg gtataactca actggttgaa
     1861 catccagcat tagatgccac taggacaggt tttgtaaaag cattagaaaa gaacggattt
     1921 aaagatggag aaaatattga tatagacttc caaaatgctc aaaatgacat gcctactaca
     1981 caaagtattg cgagtaaatt tgcatctgat aagaaggact taatatttgc tatatctact
     2041 ccatcagctc aggcagcatt taatgctact aaagatatac ctatacttat tactgctgtg
     2101 agtgaccctg ttgctgctgg attagttaaa actcttgaaa agccaggcac aaatgtatct
     2161 ggaacatcag attttgtttc tgtagataaa ggtttagaac ttcttaaaat atttgctcct
     2221 aaggcaaaaa ctataggagt tatgtacaat actagtgaag taaattccaa agttcaagtt
     2281 gatgctttaa aagaatatgc atctaaaaat ggatttaaag ttgttgaaaa aggtataact
     2341 acttcaaatg aagttaacca aggtatttct agtttagttg gcaaaataga cgtattatat
     2401 gttcctacag ataacttagt ggcttcttct atgcctatag tctctaaaat agctactgag
     2461 aataaaatac ctgttatagc agctgaatct ggtccagttg aaaaaggtgc tcttgcttgt
     2521 caaggtatca actacgaaaa actaggttac aaaacaggtg aaatggcagt taaaatttta
     2581 aatggcgaat cagtatctga tatgcctgtg gctacatcag atgatacaga tatcattgtt
     2641 aatgaagata tattaaaagc tttaggtatg gaaaaacctt ctaatgaaaa tatttcttat
     2701 gtaaagacta aacaagaata gaaagatatt ttctatatac gattaatatg cctgtttcta
     2761 taaattaatt tatagaaaca ggcatatatt tttattttaa tgtattatct atttaaagat
     2821 tatatgtaaa tgctcttaat atgattgtat aaattttact tgtataaact ttatacaatc
     2881 atatttattc ttctattttc tttattactc tacatggatt tccaacagct acagtatttg
     2941 gtggtatatc ttttgtgact acacttcctg cacctattac tacattatca cctatagtaa
     3001 ttcctggtgt tatgattact cccccaccta tccatacatt atcacctatt tttacaggag
     3061 aaccatactc tatgcctgaa tttctaagtt gtgcatcaat tggatgatag gctgtataga
     3121 tttgaacgtt tggagctaac attacattgt cacctatttc aattttacat acatctaaaa
     3181 aaatacaatc ataatttgca aagaaatttt cgcctacatg tatgttatat ccataatcac
     3241 acctaatgtt ttgttctaca tttatttgct tgccaactga accaaatagt tgtcttaaaa
     3301 tatcttctct tttttcatat tcatcctcta aagtattatt aaacaatcta gttaattttt
     3361 tacaatactc tctttctttt actaacaatt catcatttgc atagtatcct ttcccagaca
     3421 acattttttc tttttcagtc atatagttta ctcctttttg ttataattgt atctaaagca
     3481 aaataaatat tcctgtggaa aatctaaata tattctgtca cctttcttaa atctcatttt
     3541 acttttttcc aattctattt gaataggtac acattcgtta gataaatcag tttttctaac
     3601 atatatagta tatgtaaaag gattttctac aatgttaatc aattcgaaac ataacttttc
     3661 atttaaatct tcattagaat ccaagacctt aatatgatgc tccctgattc ccacatattc
     3721 tatattatca cctatttctc tatttaatgt caactcgcat ccccagtctg tagcataaat
     3781 agtattttta tccagtctat ttaatttgga tatattttta caccctgtta tcttagcttc
     3841 tataaggtac tttggagatt caaagagctc ttttttaggt ctctttggaa gaccttctcc
     3901 tttgttgtac actataatat catcacaaac tcggtaagct tcttcaatat catgagtaac
     3961 aaatagtata tctcccttgt aatcttttaa catattcata agttcttttt ccatattact
     4021 tcttaaatga tggtccagtg ctgagaaagg ttcatctaaa agtaatatat ctggagatgt
     4081 tgccaatgct ctagctagtg ctaccctttg ttgttgacca ccagataatt gccaagggta
     4141 tctcccctca aacccattta atttaagtat atctaagtac cttgctacta tttccttttt
     4201 ttcactctta cttagtttta aaagcccaag ttctatattt tgacttacag tcatatgtgg
     4261 aaacaaagca tagttttgaa ataaaaatcc tacctttcta tcttgtgttg ataaatttat
     4321 cttcttttca gaatcaaata gaactctatc atttaataca attttacctc ttgttggttt
     4381 ctcaagccct gctatacatt ttaatgtcat actttttcca gaaccagatt ctcccaaaaa
     4441 ccccagagta cctttttctt gtttaatttc tacctttagt ttaaaagagg ataaatcttt
     4501 ttcaatatcc acgtataagc tcaactatcc acacctcttt cctataattt tctgctgaaa
     4561 ttccgaccaa taatttagta ataaaatcat tgctatagat attgctacaa taatcataac
     4621 ccacatcata gctttattca tatctccgcc ttcaacagca aaaaatatag ctattggcat
     4681 agtttgagtt tttccaggta tatttccagc tatcataagt gtagcaccaa attctccaag
     4741 agctctggca aacgacagca ccaatccacc aattattcca ggccatgcta atggaactgc
     4801 aatcttaaaa aatactttcc attcattaag acctaatgtt ctagcagctg acaacatatt
     4861 attatcaatc tgttcaaaag ctgaccttga agttctatac atcataggaa aagacaccac
     4921 tactgctgaa attacagttg ctgtccaact aaatattaaa gtcctatcga aatttaataa
     4981 aaactttcct ataaatccat tcttcccaca tagtaataat aaaaagaaac ctacaactgt
     5041 tggtggcaaa attagtggaa gtgtaaatag tccatctata agacccttcc acttacctct
     5101 atagttagcc ataatatatg acacaagtat tcctattaca aaagttatta ttgttgacaa
     5161 aatagatgtc ttaagtgata tccataaggg tgaccaatct gttcccatct ttccctcctt
     5221 aattttaaat ggaaatagtg aaactaataa ctctattaat ttcactattt ccattttatt
     5281 ataatacaca attattttca attttcattt atttatatat aatttatact taattacaca
     5341 taatttattt ctaagtttat tctacttttt tatatccaaa ttcttcaaaa atcttttgtc
     5401 ctgactcact taataaaaat tcttcaaatt tcttagcagc atctacgttt ttactagctt
     5461 taattactga aactggatat gtaataggtg aatgagtttt ttcgtctgtt ttttcaacta
     5521 cttttatctt atcattattg acagtatcac tgaagtaaac gaatccaaca tcagcatttc
     5581 ctgattgcac ccatgctaaa acttctttta catcttttgc aaatactagc ttatctttta
     5641 acttatcttt taaatttaaa ttagttaaca cctcatctgc atacttacca gctggaacac
     5701 tttcagcttc accaacagca attttcttca ctttatctgt agtcaaatct ttcatacctg
     5761 atacactact gtcagcagaa gaaataagaa ctaaatcatt ttttactaaa tctttcatag
     5821 tatcagaaac taataacttc tcttcatcca aagctttcat ttgtttttga cctgctgaaa
     5881 taaataaatc acaaggtgct ccttgttcta tttgttgttg taatgagcct gatgctccat
     5941 agttaactgt aagttttaca tttgaatcaa cttttttata ttcttcttca atctttgcca
     6001 ttgcttcttt taaacttgca gcagccgata tatttaactc tactgaatca ctacttttag
     6061 ttgttttttc ttgttttcca tcatcttttt tgtcttcatt actattgcac ccaactgtcc
     6121 ccaaagttaa acatgcaact aatcctaata ttcccaatat ttttttcata acaatctccc
     6181 tttttgtaaa tttgatacac attttaacat gttttattat gttttatcat gttttgttat
     6241 atatagttta attatatata catgataata aattttcaag taatttattt acactacttt
     6301 taaatatttt gctacatttt ccttttttta atgttaaaat aaccttatat ggaggtggat
     6361 tatggactca ttatcatctt tgacacctgt cgaggtagct gaattattaa agataacaaa
     6421 aaatactgta tatgaattaa tcaaaagagg tgaacttcct tcttataaag tagggaaaaa
     6481 aataagagtt gatactaaag atgttgaaga atatatcaac agccaaaaaa ctggtaaagt
     6541 aaaggataca catttcaaca ataaaaatat agatataaat aataatttca aaattattat
     6601 ttctggtcaa gatgtaatat tagacatttt agctaggtct gttgaaaaaa aattggatgg
     6661 aataagtaca tttcgctctt atataggtag ttataatgct ttgtatgagt tatataatgg
     6721 cagagtttct atagcatcat cccatctatg ggatttcgaa acggatgaat ataatagcac
     6781 ttttgttaaa aagctattgc caggaatacc atgtgtactt ataaatctag cttatagaat
     6841 gcaaggtttc tatgttgcta aaggaaatcc taaagagata acaacctggg aggatttaac
     6901 aaaaccaagt ataaccattg taaataggga aaaaggttct ggaactaggg ttttactaga
     6961 tggtaaactt agacttctaa actttaatgg taaacatata aaaggttatg ataatgaaga
     7021 attatcacat cttggagttg caagtactgt ttctcgtgga atagcagatg ttggtttggg
     7081 aaatgaaaaa gctgctttac aagtaaataa tatcgacttt attcctcttc acaaagagcg
     7141 ctatgattta gttattaaaa aagaagactt acaaaatcca gtttaccaaa ttataattaa
     7201 cataataaac tctcctgagt ttaaagctga acttcaaggt cttggcggat atgatttaac
     7261 tgatactgga aaaactatag caaaagttta agattaaaat cgcaatctat tttataagat
     7321 ttataatttt tcacaaaatt tataacttaa acacaaaata aattgcgatt ttagtgtctc
     7381 aaaaaattta tgtctttcat tttatcatcc aatacaaaat ataatataat taattgtgca
     7441 atcatattat attaatatat atttacataa tagtattttt ctttaatgta gattaacata
     7501 tgatatgttt taaacatcaa tccccttgct ttcttatata tatgtggcaa cacatatgag
     7561 tttaataaat tatatacaat gttgctttct tcttgtattt tttcaaccaa caagttattt
     7621 ataaaattac ctttctcatt ttcaataatc tcactccaat ttatcttaac ttttaatata
     7681 tatcttccca ctaggtatac acactctaca ataaataata aaaatacaat gcagtcagta
     7741 cctgttgtct tatataaatt acccgatact aatggttttg cgaagaaatt ataaaaattc
     7801 aaagtatcac ccgtataatc aaaaagtatt aatagcactc ctagaactct caatacttta
     7861 atattcaaca tgtttttatc tttattattt ttgtttttca tatatgctac ctctcttttc
     7921 tttagatttg attgtaatct aatactaact cttttatttg aggtataaat gaatttaatt
     7981 ggtagttatt ttcattttta ctagaaatta attcacatta ctaaacaaaa cttatattta
     8041 aacataaaaa tagcctatct atcttttaat aaatataaaa ataaataagc tactaatatt
     8101 caattaactt ttatcactta aaacattact atctataaat ttaaatattt tattgaagta
     8161 tactgtacta tcatgttcaa atccatcaac atgaccaata ccttctataa gccataggtc
     8221 ttttttatca tgtttaacta aatcatacat tttcttgcta ttttgataat tacaaacagt
     8281 atcttttgtt ccatgtataa acagtatggg tacattagat ttctcaactg actcatatgg
     8341 ttttacatca ctgtaccaaa atccagattt aaacttatta tataaatctc cacaagtcac
     8401 cacataattg attaatatat ctggtattcg tttttctagt actcccattc taattgcatc
     8461 tttcatttca gaataaggag aatccagtat ataaaaatcg accttatcat cttttgaatt
     8521 tatctctgaa tgcatagcag ctgtccctgc ccccatggaa aatccatgca ctcctaatct
     8581 tccttcagga aatttatttt ttacaaattt aactagacta tctaaatcat acctttcata
     8641 aagaccaaat gtataattat ctccaccact attaccagta tttctttgat tataaactaa
     8701 tacattataa cctttatcta aatatctata tgctaccttt aacatttcat agtaactact
     8761 tccaattcca tgaacaagta tcatagtatc tcttgtcttt atatttgaag ttataaaaag
     8821 actttcaagc ttgtaattat tagaactttg tatcattaat tcagatgtct tgtagttttt
     8881 taatttctcc agtggtttat cttttctctc agaaaatatt tttactatat cttcgttttt
     8941 tattaactgc tctaaaccaa ctgaactatt atacactaat ttgccaaata tataagatac
     9001 aattaatagg attaatagaa acacaattga tataattata aatttatttc ttaaactaat
     9061 acaattttta aaactatttt tcatatttat ttaccataca attttcctat attat
//
LOCUS       DAEMHW010000154         9000 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEMHW010000154 DAEMHW010000000
VERSION     DAEMHW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.154"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            134..454
                     /locus_tag="KPK67_002912"
     CDS             134..454
                     /locus_tag="KPK67_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBG2459847.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            592..1236
                     /gene="trmB"
                     /locus_tag="KPK67_002913"
     CDS             592..1236
                     /gene="trmB"
                     /locus_tag="KPK67_002913"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBG2459848.1"
                     /translation="MRRRRKKGSDEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            1365..2111
                     /locus_tag="KPK67_002914"
     CDS             1365..2111
                     /locus_tag="KPK67_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459849.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(2240..2518)
                     /locus_tag="KPK67_002915"
     CDS             complement(2240..2518)
                     /locus_tag="KPK67_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459850.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            2793..3239
                     /locus_tag="KPK67_002916"
     CDS             2793..3239
                     /locus_tag="KPK67_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459851.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            3509..3961
                     /gene="accB"
                     /locus_tag="KPK67_002917"
     CDS             3509..3961
                     /gene="accB"
                     /locus_tag="KPK67_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBG2459852.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            3977..5338
                     /gene="accC"
                     /locus_tag="KPK67_002918"
     CDS             3977..5338
                     /gene="accC"
                     /locus_tag="KPK67_002918"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBG2459853.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            5353..6207
                     /locus_tag="KPK67_002919"
     CDS             5353..6207
                     /locus_tag="KPK67_002919"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBG2459854.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVITGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            6228..7172
                     /locus_tag="KPK67_002920"
     CDS             6228..7172
                     /locus_tag="KPK67_002920"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBG2459855.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            7361..7621
                     /locus_tag="KPK67_002921"
     CDS             7361..7621
                     /locus_tag="KPK67_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBG2459856.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            7774..8145
                     /locus_tag="KPK67_002922"
     CDS             7774..8145
                     /locus_tag="KPK67_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG2459857.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            8175..>9000
                     /locus_tag="KPK67_002923"
     CDS             8175..>9000
                     /locus_tag="KPK67_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459858.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINDLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVS"
BASE COUNT         3489 a          944 c         1526 g         3041 t
ORIGIN      
        1 taaaatattt taaagcctta ggtaatatta ggtagaagtt ttagtaaaat atgcaaaaac
       61 taattgatta tgtaccttgt atgggtatat aatagatatt gttttaataa aatatttgaa
      121 agggaatgat ttgatgaaaa ttactttacc agaaacagct attgatactt taaaaagtat
      181 cttaaaagac aatcaagata aacctaataa tataagagta tactttgcag gtgttggttg
      241 tggaggtcct tcttttggat tagctttaga tgagaaaaaa gaagatgact taacttatga
      301 agttggagag ttacaatttg taatgagctc tgatgaatat agtcagtatg gagatataat
      361 aatagaagat acaggatttg gatttagagt tattcctgaa aatatgaaag atcaaggtgg
      421 aggaggatgc tctggttgtt cagggtgcca ctaagattaa gtttttagta agtttagaac
      481 tctctttata gaaataaatt tctattttaa gagagttctt tttatttttt ggtgagataa
      541 tgtataataa aaatgtattt tactaatttt ttatatgtaa ggagaattat tgtgagaaga
      601 agacgaaaaa aaggttcaga tgaaaagctt ttaagttata caaaatatgt tttaagagac
      661 gatatagaca aattaaaagg caagtggaat ttaaagttta gaaatgataa tccaatacat
      721 gttgaatttg ggactggaaa aggtaagttt ataacaactt tagctaagca gaaccctgac
      781 ataaattata ttgccatgga acttaaggaa gaagttcttt taaaggctgt tgaaaaagca
      841 gatgcttcta atttgaataa tatactattt ttatggggag atgtaagcaa tattttggat
      901 tactttgaag ctaaagaatt atcgagaatt tatataaatt tctgtgaccc atggcctaaa
      961 aatagatgga gtaaaagaag actcactcat tctggatttt tagaaatgta caatagggtt
     1021 ttggaagatg atggcgaaat tcactttaaa acagataatg aaaaattatt cgagtttagt
     1081 ttaaatgaaa ttgctgcaaa taattggcta ttaaagaata tttctcttga tttaggaaat
     1141 agtgagtatg aaaataatgt tacaacagag tatgaagaca aatttatgtc acaaggtatg
     1201 cgaatattta gatgtgaagc taaaaaacgt aactagtaat atgtttataa gtttaatagt
     1261 agaaatttat tttaatttat attaataaag tgtacgaaag taggtatata taacaaggaa
     1321 acagaaccct ctcatagaaa ttaaattatt ataataaaaa atttatgtgg gagggtacta
     1381 tgaatttagc taatttaata ccatttatta tttctgcagc agctttaagt ccaataaatt
     1441 caaatgataa ggttttgagc gaaaatcaac ttaaagatat ttttcctgat gcaaatttaa
     1501 gagcagttgt aaaaagatat ataaacccag atgagatgac tataagcaat ataaaggctt
     1561 tagatggaga attttatgca actggtgaga gcatttcaaa tttaaaaggt atatcatatc
     1621 ttgaaaatgt agacaatttt attttttgga ataataatat aaaagaggtt cctaaggaag
     1681 ctttaagtct aaaagatatg gattcaataa atcttgccaa taattatttg attgatgacg
     1741 atgtagtaaa ttctctttct cataatggtg tggatgtaaa ttgcgattta aattttattg
     1801 atacaaaaga taatcagtat aaactggact ctaaatatca taatgttgat atagcaaaag
     1861 gtgaaagtat tgatttgaga aaaataatta cgaagaaaat tgatagttat tataaatact
     1921 gggaagttac tgataacctt ccaaaagatt taaattttat tgtatctgta agtgataaga
     1981 gtgtactttc atgtgaagat atgataatta aaggtaataa aacaggtcaa gcagtggtaa
     2041 aagttatact aagcgacaaa aatgatttaa atacatctca agaagttcta attactgtca
     2101 atgtaaaata atctgtcaac ttattcaatc attctatttt caatttaagt gtataatcaa
     2161 aataaaggtt gttagtaaca gaacgctaat attagtgctt tgttactagc aacctttaaa
     2221 ttttatagaa atagattatc taaaaaattc catttcttct gttagtagcc catattccta
     2281 ttaaaattat cagtgaatat attatataca aaactattct tatatattgg acatctaatc
     2341 caaaaaattc aatgttactc tgtgaggtat gcaacatcac aaggactgca gatataccaa
     2401 taagcaaagg attgattact atttgcttat cagttcttaa aatcattatt ttacccatcc
     2461 aaattattga taatataaaa aataaaattt taagaccaaa ttctaatgta tctaccaaag
     2521 cgaaaacatc ccttcattta taataaattt ttcagctcaa caaaatataa aaccctacaa
     2581 acaacttttg aatccataat aagataactt gattaagcac tatgaaataa gtactataaa
     2641 acttcatcta cattaatata tactacacta tacttcaaat ttcttaaaca taaattaatg
     2701 aaattaacaa aattttattc agaaacatat aaagatatat tttactattt aaatgcatat
     2761 cattatatat acagttttag gaggtgaggt atatggaatc taaatttttt aattggtaca
     2821 ctcaatcctt agggggaact tttggcatta tatcatgtat atgcgcatat ttaaatggat
     2881 acatgtttgt ttatacaaat agtgctagca aatattttga aagtatagga tttttgggaa
     2941 taatttcaag ttatacacta cttccacttt gtatgcttac tttgattttt ggagttatat
     3001 attcttatac tccagaaaca tccgacaaaa gtatttttaa tatacctttt tttactttaa
     3061 ataaaagatt aatacttatt acagtagttt ttggttttat aggtgctaga gtttacttta
     3121 taattcctgc aattctaata acttttaacc tatatagcaa tgttttgata gctaaattaa
     3181 agtacatcct atgtaaaaat aaagaaaatg atactaattt gaaagaaagc gaagtctaaa
     3241 ttgcataata ttggtatttg tcttaaaaat tcccgaggta ttaatttgta ttctcttgta
     3301 ttttgcaaaa aatattgaat tttattcatt ttatacttat aatttatatt aataatggaa
     3361 aattaaaaca aatataagtt actggttgtt aatttaaaat taaaagatat attgatgtgt
     3421 tgattctcta gtcagcactt tttcttttat aaatttgttt tttgtgatat aatattagca
     3481 ccaggtaata atacaagggg gtaacaaaat gaacatcaat gaaattaagg aattattaaa
     3541 ggccatagac tcaactaact tagaatatgt aaaacttgaa agtagcgatt taagattaga
     3601 agtatctaaa aaagctcaat ctacaagttc acctgtttta agtgtacaac aagaatcagt
     3661 agtggatctt tctttagaaa agccagttgt aaacgataca cctgtcacat ctaatgagaa
     3721 tctatcagta gtagtagctc ctttgatggg aacattctat gattctccaa gtccagatgc
     3781 agatagcttt gttaaagttg gcgatgtagt tgaagaaggc gacactttat gtatattaga
     3841 agcaatgaag cttatgaatg agataacaag tgaaattaaa ggtgaaataa ttgaagtatt
     3901 agttagtaat gaagaactag tagaatataa tcaaccttta tttaagataa aaccattgta
     3961 gtaggaggta tttaagttga taaaaaaaat actagttgca aatagaggcg atatagcagt
     4021 tagaatcatc agaacatgta aagaactagg tataaaaact gttgctatct attctgaaat
     4081 agataaagat tgttttcata gatatatagc tgatgaatct atttgtatag gaccaaataa
     4141 tataagtaag agttataata atatagaaaa tataatctat cttgctctta aattaaagtg
     4201 tgatgctatt catccaggct ttggattttt atcagagaat cctgaatttg caaagcaatg
     4261 tgaagataat aatattattt ttataggtcc aacaagagaa caaatgattt tgatgggaga
     4321 taaatctaga gcaagagaaa ctatgatgga attgaatatc cctgttgttc ctggttcaga
     4381 atctgttttg aaaactaagg aagaagcttt agaagttgca agagaaattg gatatcctgt
     4441 tatgataaaa gcctcaagtg gaggcggtgg aaaaggtatg agaatcgtta gaaaagaaga
     4501 agaacttttt tcaaattttg atatggcaag ttcagaagct ctagcagcat tttctaatag
     4561 tgatttatat atggaaaagt ttatagaaaa tcctagacat atagaagtac aagtatttgg
     4621 agataaacat tcaaatgcaa tacatttagg tgatagagat tgttctatgc aaaggcgaaa
     4681 tcaaaaagta atagaagaat ctttaagtcc atacctttca gatgaagaaa gacaaaaact
     4741 tcataaaatt gcagtagata tagtaaaagg agtaggttat atcggtgcag gtactattga
     4801 gtttatagtt gacaaagata aaaactttta ttttatagag atgaatacaa gaatacaagt
     4861 tgaacatcca gttacagaga tggtaacaaa tcttgacctt ataaaacttc aaatatctat
     4921 agctaatggg gataaaattc cttttaaaca agaagatatt acttttagag gtcatgctat
     4981 tgaatgtaga ataaatgcag aagattcatc aaagaatttt gctccatcac caggtaagat
     5041 agaatcatta aatttacctg gaggttttgg tgttagattt gatacttttg tatatgcagg
     5101 atatactata ccacctttat atgattctat gataggtaag ttaatatgtt gggctgaaac
     5161 tagagaagaa tgtataagta gaatatatag agctctagac gaaattattg tagaaggtat
     5221 taatacaaat gtagaatttc aaaaggcact tgtaacaagt gaggagttta gagaagatac
     5281 tcatcatacc aaatttatag aagatgtctt tatgaagaaa gaatttgcta cattataagg
     5341 agtaaataga ttatgataaa aaaattttta tcatcaaaag aatctaagta tgttacgatt
     5401 tctttagatg acaactttaa aaagaatagt gtagatgata aattttggac ctattgcaaa
     5461 ggttgtgata gtcatgtatt tagaaaggac atagaagaaa attcatttgt atgtccaaaa
     5521 tgttcaagac attatggctt aagagcaaga aaaagaataa atttattgat tgataaagga
     5581 acttttatgg agtttaattc tgatgtagaa tttcaaaatc cactaaattt tcctaaatac
     5641 aaagaaaaag tagactctta taaggaaaaa acaaaagagt cagaagctgt tatcacaggt
     5701 tatggaagaa taaatggaat taaaacagtt atttgtgtaa tgaatcctga ttttatgatg
     5761 ggaagtatgg gttctattgt tggggaaaaa ataacttatt caatagagta tgctgctgaa
     5821 aataatttac ctataattat ttgttctgct tcaggaggag ctagaatgca agaaggtatg
     5881 gtatctctta tgcagatggc aaagacttct caagcacttt ctaagcttga agaaaaatca
     5941 ttgccatata tatcagttct aactgaccct actactggag gagtaactgc tagttttgct
     6001 atgcttggtg atattataat ttctgagcca aatacgttga taggttttgc aggtccaaga
     6061 gtaatagagc aaacaataaa tcaaaaactt cctgaaggat ttcaaacttc tgagttttta
     6121 cttgaaaaag gttttattga catgatagta gatagaagaa aaatgaagga agtattatat
     6181 caaattcttg ctatgcacaa gaaatagggc ataaaggagg aactgttttg attgaaaata
     6241 atagtgaaaa aataaaagtc ttagaaaatg atattaaaca attaataact atttctaagg
     6301 caaataatat agatttaagt gataaaataa attcacttaa tgagaaattg gaaaagctta
     6361 aggaagatgc tttttcacat ctatcagcat atgaaaaggt aacattaagt agagatataa
     6421 agagacctac gacattggaa tacatagaac atatttgttc taatttttta gaacttcatg
     6481 gagataggct ttttaaagat gacccttcta tggttggagg aattggtcag ataggaaagt
     6541 ttaatgttac tatagttgga caccaaaaag gaagagatac aaaagaaaat ataaaaagaa
     6601 attttggaat gcctcatcca gaaggataca gaaaagcatt aagacttatg aaacaagctg
     6661 aaaaatttaa tagacctata ataacgttta ttgatacatc aggagctttt tgtggtctag
     6721 aagcagaaga aagaggacaa ggagaagcta ttgcaagaaa cttacttgaa atgagtaaac
     6781 tttctgttcc agtaatcact tttgtaattg gtgaaggtgg tagtggagga gctttaggta
     6841 tcggagtagg aaatgatgta tgtatgttag aacattcagt ttattctgta atttctccag
     6901 aagggctttc tagtattcta tttaaagatt catctaaagc taaagaagct tgtgatgtta
     6961 tgaagcttac aagtaatgat ttatatgatt taaaaataat agataagatt ataaaagaac
     7021 ctcttggagg agcacaaaaa gatgtagaag ctgtttcaaa agaaataaaa gcttatattt
     7081 tagaaagatt aaatcattac aaagatatgg ataaagagga aattatagct caaagatata
     7141 aaaaatttag aaatattgga aaatgcttat agaatataaa gaaatttgta gttttttcat
     7201 aataaaatgg ttaaaaaaaa taaaggtttt cttaaaacga ttatagaagt atatttttat
     7261 aaaagtatac tttaaatgat gattgtaatt agtaaatggc aattatgatt tagtacttta
     7321 ggtaatggat actttaacaa attaacaagg agatgttgaa atggaggtac taaaagtttc
     7381 atcaaaatct aatcctaatt ctgtagctgg agccttggct ggagtactaa gagaacgagg
     7441 cgttgcagaa atacaagcta taggagctgg agctctaaat caagcgataa aatcaattgc
     7501 aatagcaaga ggttttgttg cacctagtgg aatggattta gtttgcattc ccgcatttac
     7561 tgatatagaa atcgaaggtg ataagaaaac agccattaaa cttataattg aacctagata
     7621 atatatctct ttattagaat ttaattaagt tttaaaggtt ggctatagaa ataggacact
     7681 atagtgaacc ttttttactt tatatgtttt attttatgta acttttaatg tataatctta
     7741 agtaaacaaa taagattgga ggaaatatac attatgaaat taaaattatt ggtagaagag
     7801 tatgcagtat gtagattaaa taatgacagt aaaataccaa catggataga tacagagaag
     7861 ttttactcta taactagaac tgatgatgaa ttaagtgttg tatgtttaaa taataatatt
     7921 ccttctgatg tcaagtcaga aaaggagtgg agaatactta agatactagg tcctttagac
     7981 ttttcacttg taggaatttt atctaagtta agtagtttgt tagcagacaa caaaataagc
     8041 atatttgcaa tttctactta tgatacagac tatatcttag taaaagaaaa agatatagaa
     8101 aatgcttgta aaattttaag ctgtaatgga tatgaagtag agtagttaaa tacttttaca
     8161 tgaggactta acttatgatt tatataatcg aaattttaaa aataagtgta ataaatattt
     8221 tttgtttcat aggaatattt attgtatttg gattgatttt tagcattaca gaaaatctaa
     8281 ataataaatt tatattttca tcttttggaa aaactggaat tattgtaact ggagctatag
     8341 gaacatttgt tcatgaattg agtcatttga taatgtgttt aattttcatg cataaaataa
     8401 attcagttaa gtttttcaga ccaatagaaa gtaaaaatga tggtatacta ggatatgtaa
     8461 gtcatagcta taaaaaagat aatctttatc aaagtatagg aaatttcttt ataggaattg
     8521 cacctattat aggtggaaca ataatgatta ttttaatttt taagatatta ctgccagata
     8581 gttatatgaa agtgactcaa aatatagatt taaagttata tgtaagtatg attaatgatt
     8641 taaatataaa tggttttata cacattattt taggtgatat ttccaatttt ataagaataa
     8701 tgttgttaac acctagtgta tattcattga agtattttat atttatgttt ttaatgtatt
     8761 ctatatctac acatatgtca cttagtagtg ctgatttaaa aaatagctta aatggtctga
     8821 gctttatagt tatagttatt tttgtaataa gttcaattac atacttattt ggatttgata
     8881 acttgaatat atccagttta atagttaagt ataacatatt tataagtttc tttatggcaa
     8941 tagggtttat tttttcaata gtaactctca taatatccta tattttaagc ttagtcagcc
//
LOCUS       DAEMHW010000155         8990 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEMHW010000155 DAEMHW010000000
VERSION     DAEMHW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.155"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            225..1241
                     /gene="gap"
                     /locus_tag="KPK67_002924"
     CDS             225..1241
                     /gene="gap"
                     /locus_tag="KPK67_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG2459859.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            1636..2805
                     /locus_tag="KPK67_002925"
     CDS             1636..2805
                     /locus_tag="KPK67_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459860.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            3186..7439
                     /locus_tag="KPK67_002926"
     CDS             3186..7439
                     /locus_tag="KPK67_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG2459861.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            7588..8082
                     /locus_tag="KPK67_002927"
     CDS             7588..8082
                     /locus_tag="KPK67_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG2459862.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            8198..8575
                     /locus_tag="KPK67_002928"
     CDS             8198..8575
                     /locus_tag="KPK67_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBG2459863.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT         3624 a          936 c         1629 g         2801 t
ORIGIN      
        1 atgtcccaag ggacattgag taaaataaat gatttcagct tctttacact aaaaagtaca
       61 tactaaatga ggggtaaaat aaagaaaaat gatatactaa ttgtcttttg ataataaaaa
      121 aacatggaat ttgattattt aatcaagaaa aagtattgaa caaatgattt atttagtgta
      181 caataatcat gtaaaaacat agtaatggag ggagacattt cataatgaaa attaaggtag
      241 gaatgaacgg atttggaaga ataggaagag ctgtattaag aatagctcaa gaagaattag
      301 gagataatat agaaattgta gccataaatg caagagcaac aacagaatct ttggcacatt
      361 tatttacata tgattcatgc tatggaactt ttagaggtga agtagaggca aaggatgaag
      421 acactctagt agtaaactct aaagagataa aaatattaag atataatgac ccagaagaat
      481 taccatggaa agaactgggg gttgatatag taatagaatc aacaggatta tttacacaaa
      541 gagaaaaggc agaaaagcat attaaagcag gagcaaaaaa agtaattata acagctccag
      601 gaaaaaatga agatataact atagttatcg gagttaatga gaaagaatat gataatgaaa
      661 agcacaatat aatttcaaat gcatcttgta ctacaaattg tctagctcct ttcgctaaag
      721 tattggacga aaaatttggt atagtaaaag ggttaatgac tacagtacat tcatatacta
      781 acgatcaaag aatattagat aaaagccata aagatttgag aagagcaaga gcagcggctg
      841 aatctataat acctaccact acaggagcag caaaagctgt gtcaagagta ctaccacaat
      901 tagaaggaaa attaaatggt ttttcactta gagtaccaac accaacagtt tcacttgttg
      961 atttagtgtg cgaacttaaa gaaaacgtta catctgagca agttaactct gtgttaaaag
     1021 atgctgctga aggagaatta aaaggtgttt taggatattg tgacaagcca cttgtttcta
     1081 tagattataa aggagattca agatcatcta ttatagacgc tctatccaca atggttatag
     1141 aagacaatat ggttaaggta gtttcttggt atgataatga atggggatat tcttcaagaa
     1201 ctgttgactt agtaaaatat gtagctgaaa aattaaaata atatatattt cttaaagcaa
     1261 attttaaaaa aattttttaa aaaaattata taaaactaaa aaaaaagatt atgaaaaata
     1321 ttgaaggaaa aattttttca taatcttttt tctttcatgc aaaaagatac aagttacaac
     1381 ttatatttgc atattaggtc gttagagaag tatatatata tttagtaagg aatcatactt
     1441 tttataaaat gaaagataag atgtcgaaaa cttgcataaa tctgaaaaat ttttattatt
     1501 aaaatactga tttccatttt aaaaatgata aaaaaatgac aaataaagtt gcaaatagta
     1561 atacttataa gtataattaa aaaagagaaa aggttttgtt aaactttata acaaaaaaag
     1621 aagggatgat tcaaaatgga gaagttaaca gacaggagac aggttaaggc agtaagcatg
     1681 gaaaccttca ttttcctaat attactagta gttggttttg ggtatgttgg tagtattatg
     1741 ggagcaggaa tgatgttcaa agtaataatg agtacagcac atgctttact attggagaca
     1801 gttttcttaa ttatggctat ggcagttctt gcaggcgcat taagcgcact actttcagag
     1861 tttggagtaa tagcgttaat taataaaatt ttcgctgtat ttatgaagcc tttatatgga
     1921 ctacctggtg ctagtatagc aggagctata acaacttatt tatcagataa tccagctatt
     1981 ataccttttg ctaaagataa aacattcaca cagtatttta aacaatacca agttccagca
     2041 ttatgtaact taggaacagc ctttggtatg ggacttatct taacaacatt tatgatttct
     2101 caaggtacag aatatgtact tccagcatta attggtaatt taggtgcaat tataggtagt
     2161 gtcataagtg ttagaataat gttgacattt actaagaagt tttataaata caatccggaa
     2221 gaagataaag caacaggaac attagagaaa aaggatgaat ttagagaaat cagagaagga
     2281 aatgtatttc aaagagcttt agatgcaatt cttgaaggtg gtaaaatggg ggtagatatg
     2341 ggaatggcta taattcctgg agtcctagta gtatgtacat tggtgatgtt attaactttt
     2401 ggaccatcta cagaccctgt tacaggtcaa gaagtttata caggagctgc atatgaagga
     2461 ataaaattac ttccagttat tggagataaa cttggattta tattagaacc attatttgga
     2521 tttacatcac cagaggcaat agcattccct ataacttcat tgggagctgt tggtgcagca
     2581 atgtctctag ttccagagtt tataaagtca ggagctataa ctcctaatga tatagcagta
     2641 tttacagcta tgggtatgtg ttggagtgga tatttaagta cacatattgg tatgatggat
     2701 gcattaaatg ctagacagtt ggcaggtaaa gctattcttt ctcatacaat aggtggttta
     2761 tgtgcaggag cagctgctca ctttatattt acactcgtag gataaatgta atcagctaag
     2821 ataaaaaata atatacaaaa aaatggttgt tgatatgtaa taagaattta catatcaaca
     2881 accatttttt tctgactaaa gataatttat aatacataaa cattaagtgg atattagagt
     2941 taagttatat ttatgtaaaa agtaacaaaa atctgtagaa atttatgaca aaatttagta
     3001 cactaactat atgttcttat ttaaaattta aggttagaat taggaattac ctagtagagt
     3061 aagctttgat taataaaaga atcataaaat aaaaatatta gtcataaaga ttataaataa
     3121 cttacttaaa ataaagccat agaacttaca aaaattaaaa aagcatataa atatggaggg
     3181 gtacaatgga tgaattattt catataggaa ttgatgtagg ttctacaacg gtgaaggttg
     3241 tggtacttaa taatagtaat aatatagttc ataaagaata tagaagacat tattctgatg
     3301 taaaaaaatc agttaaagaa gtactaaatg gtatatatga aaaattggga gatataaata
     3361 ctaccattat aataactggt tcaggtggaa ttggaatatc aaaaaaatta ggtgtaaaat
     3421 ttgttcagga agttatatct agtacaaaat caatagagta ttttcatcct gaaactgatg
     3481 ttgtcataga acttgggggt gaagatgcaa agataacata tctaagtgga ggaatagacc
     3541 aaagaatgaa tggaatatgt gcaggtggca ctggagcatt catagaccag atggcatcac
     3601 ttttaaagac agatgccagt ggattaaatg aacttgctaa agggtataat gttatatatc
     3661 ccatagcttc aagatgtggt gtttttgcta aaacagatat tcaacctctt ataaatgatg
     3721 gagccaatca gactgatata gcaatgagta ttttcaatgc agttgtcgta caaactgtga
     3781 gtgtcctttc ttgtggaaga aaaatagaag gaaatgttgc gtttttgggt ggtccattgt
     3841 actttttatc tgaacttaga gaagcattta aaagagtatt ggatttaaaa gatgaaaaca
     3901 taatcttccc agaaaatgca caactttata ttgctattgg agcaggatta ttatcagtag
     3961 atgaagataa tatacaacta aaatctctaa tagaaaaatt ggatagtata aagaatattg
     4021 aagatggaga agtaaattta ttagaacctt tatttaaaga taaaaatgag tatgaagaat
     4081 tttcaagaag acatgaaaaa gaaaaaataa attatgtgga cataaattca ataaaaagca
     4141 attgttattt aggaattgat gcaggctcaa ctacaactaa agcagcactt attgatgagg
     4201 atggaagact tgtatattct tattataata gcaatgaagg aaatccatta aaaacaacca
     4261 ttaaagttat aaatgaagta tatgatatat taccaaaaaa tattaaaata ttgagttcaa
     4321 ctgtaactgg atatggagaa ggtcttatta aaaaagctct taaaatcgat aacggagaga
     4381 tagagactat agcacattac aaagctgcta aatttttcaa taaagatgta gattttatac
     4441 ttgatattgg tggtcaagat atgaagtgtc taaaaataaa agatggagta attgataata
     4501 taatattaaa tgaggcttgt tcatcaggtt gtggctcatt tttagagaca tttgcaagtt
     4561 ctttatctat gacaatagaa gaatttgcac acgaaggtat ttattctcaa aatcctgtag
     4621 atttaggttc aagatgtact gtatttatga attctagagt aaaacaagct caaaaagaag
     4681 gtgcaagtgt tggagatata tcagcagggc tatcttattc agtaataaaa aatgctttat
     4741 ttaaagtaat aaagattaga gatttaaatg agattggtaa taatattgtt gtacaaggtg
     4801 gaacctttta taatgatttg gtgcttagaa gctttgaaaa actaattgga aaaaatgtaa
     4861 ttagacctaa tatttcagga attatgggag cttttggttc agcacttata gctaaagaaa
     4921 aatatgaaag tggatacaaa acatccttgt tatctaagga agagttgaat aatatcaagc
     4981 tagaagcaag tgtaactaga tgtaaaggtt gttcaaatca ttgtcttctt acaataaata
     5041 aattttctga taatgaaatt tttatttcag gtaatagatg tgagaagggt gaagctattt
     5101 atggtgataa aaaaatagga ttagaaaaga aaaaaccaat aaatttattt aagtacaaat
     5161 acaatagaat ttttagatac aaaccattgg aaaaagaaga agcaaaaaat ggggaaatag
     5221 ggataccaag agtattaaat atgtatgaag attatccatt ttggtttact tttttcaatg
     5281 ctatgggctt tagagttgta ctttctgata ggtcatcaaa acagttatat gaaactggta
     5341 taactagtat tgcatctgaa acagtttgtt atccaggaaa attagttcat ggacatatag
     5401 aaaatttaat tgcaaaaggt ataaaaaata tattttatcc aagtgtaaca aatgaaaata
     5461 aagaggattc aaatgcagat aattattata attgtcctgt tgtaatatca tattctgaag
     5521 taataaaaaa caatgtagag aatataagaa ataagaacat aaattatata aatccattta
     5581 taagtctaaa tgataaggaa aaattgaaga aaagactata tgatgaatta tcaagacatt
     5641 tttctgatat aaaaataact aaagaagaag ttaatcatgc agttgataaa gctactgatg
     5701 aacagaattt atttaaaatg gagatacaag cagcaggtga aagagcactt aaagaaataa
     5761 aagaaagaaa tatgaagggg attgtgttat gtggaagacc atatcatata gacccagaaa
     5821 taaatcatgg aatgccagag cttataaatt cattagatat ggcagtgctt actgaagact
     5881 ctatatgtca tttagccaat gtacaaagac cacttagagt tgtagaccaa tgggtatatc
     5941 attcaagatt gtataaagca gctagttttg taagagataa aaactatttg gaacttgttc
     6001 aacttaattc atttggctgt ggattagatg cagtaactac agaccaggtt caagaaatac
     6061 ttaatgaaaa atcgaagata tatactatta taaagattga cgaaggcaac aatttaggag
     6121 cagctaagat aaggatgagg tccttaaaag cagctatgtt tgagagagaa agcaaaaata
     6181 tagatgtaaa aaatataaag gtaaacagga tacagtatgc taaaaataat cagataacga
     6241 acaaacatac tatattagca ccacaaatgt ctccaataca tttccaattc cttgaaaaag
     6301 ctgttaactt aagtggttat aatatagaaa ttctaaaaga tactgatagt agtgtaattg
     6361 aagaagggtt aagatatgta aataatgatg catgttatcc agctataata gttgtaggtc
     6421 agctaataac agcattaaaa tctggcaaat atgatttaaa taacacttct gttactataa
     6481 ctcagactgg tggaggttgt agagctacaa attatattgg attcttaaga aaggctatat
     6541 atgatgctgg atttaaagat gttcctgtca ttgcacttag tgtaaatgga atagaagata
     6601 gtggtataat ggataatata tcactaaaat taatcaatag actctttatg tctgttgttt
     6661 atggtgattt gctgatgaaa gtactatata gagtaagacc atatgaaaaa gttactggaa
     6721 gtgcaaatgc tctatatgaa aaatgggttg atatatgcaa atcttctctt gttaaagcaa
     6781 aaatttctgt atttactaga aatataaaag atatagtaaa agaatttgat aatttagaga
     6841 tactagatat caaaaaacca aaagtaggtt tggttggaga aatattggtg aaattccatc
     6901 caattgcaaa taataattta gtagatatat tagagcgaga aggtgctgaa gcagtcgtgc
     6961 cagaccttac aaatttcttc ttaagttgtg cctttaatac tatatacaaa cacactcatt
     7021 tagaaggaag tagaaagagt agaatgatag gagaagcatt tatatacata actggaatat
     7081 atcaaagagt atataaaaaa gcacttgata agagtgagag attttatgct ccagccaata
     7141 taaaaaatgt tgctaaaagt acagaaccag tagtgtcatt aggtaatcaa actggagaag
     7201 gttggctttt aacaggtgag atggttgaat tattaaatga aggtgtagaa aacattattt
     7261 gtatgcaacc ttttggatgt ttacctaatc acataattgg taagggttct ataaaagaat
     7321 taaagaggtt gtataaaaat gcaaatataa tacctattga ttatgaccct tctgcaagtg
     7381 aggtaaatca acttaacagg ataaaactta tgctttctaa ggcttttaaa aatatataag
     7441 atttatattt tgatatgtta aaagttaatg aatttaaaaa tatactctat attaagtatc
     7501 tataaaaaag agttatccta ttttttaggg taactcttta ttagttaaaa aattatatgt
     7561 atactgatat taaagggggg aagtaatatg agtttagtat atgatgttta taaatcaaaa
     7621 ttaggaaatt tgtacatatt aagtgaagat ggttttatag ttagtatata cataggagat
     7681 gaaaaattta ataatttaaa aaatgaatgt attaaaaaag gaattgatat aaagagaagt
     7741 aaggaagatt taaaggaagc aataagtcag atagatgatt actttaatgg aaaaagaaaa
     7801 gtctttaatt taaatattcg tattgaaggg actgaatttc aaaaatcagt ttatagggaa
     7861 atgttaaaaa taccttatgg agaaacattg tgttattcag atatagcaag aaatataaac
     7921 aatccaaaat cagtaagagc aataggacaa gcaagtaaaa gtaataaaat tccaataata
     7981 ataccttgtc atagagtagt tggcaaaaaa gatattggtg gatatatggg gaatcattct
     8041 gatttgaaag agatactatt aaatttagaa agacagagtt aacttagaaa gaaatgtttt
     8101 tattgttaat atttgatata ataatagtaa aaagaagtat tttttacaaa aacaataaag
     8161 aatttatttt aatttacaaa aatgaggggg aattttaatg tgtgataaag ttatagcatc
     8221 aaaatatcat gagaagggat ttaattgtgc agaatcagtt ataaaagcat ataatgagga
     8281 atttaataca gatataccag tttgtttagg aagtggactg ggttcaggat gtggggttgc
     8341 tagtttatgt ggtgcagtaa atgcatcaaa tataataatt ggatatgtaa aaggcagaaa
     8401 ccacgaagat gaaagtacta aagctaaagt atatgctaaa gatttaacta caacagtaag
     8461 aaaagagtat ggttcagagt tatgtataga cctaaaaaaa gatttagttg cttgtagaga
     8521 aataatggat ttcgcatatg atagtctaaa ggaaacttta aaaaaagaat tataagaaaa
     8581 taagctatca aatatgaatt atttgatagc ttatttttat agtcgatact aaataaatca
     8641 aatatatttt agaattatta tttgtaaata aacagcaaat aattttaggc tttttattta
     8701 atatcgtttt tatttatatt tatttttgaa tatgttatac tttgtttaaa taaatatttg
     8761 taatgaggga gatgagaaga ttgaagaatc tcaatttact tattaataaa ttatattcaa
     8821 aaaatcataa tgaagcatat aaaacttttt tattcttaga aaatgaaagt ttaaaatcaa
     8881 atattacata ttgttttttt gactcattcc tagaaatgat tgataatgaa aactcatata
     8941 ttcgaacaag gggattgtta ttaatctctg caaatgctca gtgggatatt
//
LOCUS       DAEMHW010000156         8947 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEMHW010000156 DAEMHW010000000
VERSION     DAEMHW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.156"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..367)
                     /locus_tag="KPK67_002929"
     CDS             complement(<1..367)
                     /locus_tag="KPK67_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG2459864.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAM"
     gene            1272..1757
                     /locus_tag="KPK67_002930"
     CDS             1272..1757
                     /locus_tag="KPK67_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459865.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(1957..2496)
                     /locus_tag="KPK67_002931"
     CDS             complement(1957..2496)
                     /locus_tag="KPK67_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2459866.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(2489..2902)
                     /locus_tag="KPK67_002932"
     CDS             complement(2489..2902)
                     /locus_tag="KPK67_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2459867.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIDTLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(2938..4059)
                     /locus_tag="KPK67_002933"
     CDS             complement(2938..4059)
                     /locus_tag="KPK67_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG2459868.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEIAKKFDSFKFIEEKKKEGKIKDIGFSFHDSAELLDKVLTEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLF
                     NDYNPDMSIPSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFMQEEYNVID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            4669..6321
                     /locus_tag="KPK67_002934"
     CDS             4669..6321
                     /locus_tag="KPK67_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBG2459869.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKIENIAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYQNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            6620..7369
                     /locus_tag="KPK67_002935"
     CDS             6620..7369
                     /locus_tag="KPK67_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459870.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVLEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDILTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFDIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYAASFANRILIFKDG
                     HIIKELNKQPNNRHTFYELIIQEIAKLDTKR"
     gene            7382..>8947
                     /locus_tag="KPK67_002936"
     CDS             7382..>8947
                     /locus_tag="KPK67_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2459871.1"
                     /translation="MNIFYMAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENILICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFLYKTSLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYSLS
                     LDILRGAKSVWISIGFYQMGLITMIIIVIGTVLFINSFLPYIMQKSKKNKRAFYTGTK
                     IITIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVRQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYQLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSN"
BASE COUNT         3281 a         1290 c         1189 g         3187 t
ORIGIN      
        1 ccattgcttc aatttctaaa actgcatctt ttggtaattg agcaacttgt acgcatgacc
       61 ttgctggtgg attttcttta aaatatgtag catatacttc attaatatca ttaaaatcct
      121 ttaaatcttt tacgaatact gtagtcttaa atattttatc catacttgta ccagcttgtt
      181 ctaatattgc ttttacatta tctaaagaag cttttgttgc ttcttttggg tcagatacta
      241 actcgccagt cttcatatct attggaagtt gacctgatgt agaaactatg tttccaactt
      301 gaattgcttg agaatatggt ccaattgctc ctggagcttt ctctgtagct attactactt
      361 ttgacatgac ttcacctcct attattaagt tatatttaca ggaaaataaa atttcctaaa
      421 caccaatatt aatacgaacc tttagattaa atttcaaatc taaaacttca aataatcaat
      481 acttagatga ttatgtttat ttgtgtcttg ttaactttta ttaaagcaat taacgtgcca
      541 aactttattt atatataaaa ttttgtgaag tcattcaaaa tagttacttc tgaatttttt
      601 cgtaaaaaaa tgctttaaaa ttactttttt atgatgtcaa tttttttctc atatctagaa
      661 atttaccaac atgagaattc ttgttttgag aatttacaga ttaaagttat tttttaacct
      721 taaattctga acctcccatg actaaagtca cagggttcct gcttcataga attttgcata
      781 atcaagttat tacatatgaa agaactatag ttgacaatcc cactattata aataataaaa
      841 cctttaccca taccttattt accctagtta tcatagagcc tattgaaaat ataacagcta
      901 aatataataa taaccaaaaa attatcaata tctaccactg ttgctgaaaa tccaaaaaac
      961 taccttttga tagtattact taacaatcta attaatctca taataaattt acaacttata
     1021 aataaattgt tatattggcc gtatctcaac aactttctcc tttcctagtt ttaaatcttt
     1081 tatagaattg taccacgtct aagtaaattt tcatactatt aagtcttatt tctaataaat
     1141 cattagctct cattctatgc ccaataatgt aagattgcag taattttatt gattttaata
     1201 gttttattta atatacttat attaattatt ttcatatgaa attattattt atataggggg
     1261 atttttttaa tatgagcaaa agtgaattaa cagcagaaac aacagaagaa atgttagaag
     1321 tactaagtgg taaagattat gatattgcat gtcatttaca tgaacttggt aaatcattag
     1381 attgtaaaat tgaaccaaaa acaggtgctc gttcttacaa aatagtatat tcaactaaga
     1441 aaccaaaacg tagcttattt actattgaat gtaatgaaaa aaaatggaga gttaaagcaa
     1501 atctttttca tttaaataca tataaagatg ctgtggagga atgctctaaa actattaaag
     1561 atagtataac taaaactcgt acttgtaaga aatgtaattc aaagtgtatt ggaggttctt
     1621 cgtttgaatt agatggaaag tcttacctga cttgcatagg aagtggtcat tattttgcaa
     1681 atatggaaga aatggattgg aaaaacctag aaaaattaat tactaaagaa aataatatta
     1741 tgcaggaatc tgtatagttt tttaaggata attattaaaa tatatgagta acaacagata
     1801 cttaaatagg cataaaaaaa taaagggtat aataaggata tagaataaaa ataaatgata
     1861 tagcacaaag aggctgtctc aaaatacaga taagcctctt tttattgttg aaatgataag
     1921 tatatattca attttataca attttataca aacatactac gccaacttac ccatttcata
     1981 tgcctgtttc attgccttac ttcccttaat atcacctaca ttccaagcac ctacaccata
     2041 tactatacct ttttccttag aaccatttag acatgatgta aatcctctaa atccttctat
     2101 tgttctttcc atatcagcca cattttcatc tgctgctgta acgataaaat agaaatcttt
     2161 atcacaaatt tcagtatatc tagagcaagt tctatcaatc atagttttca tttgcgcatt
     2221 catagtataa aaataaacgg gagtcgctaa aacaatcaca tctgccccaa tcattttttc
     2281 aagaatttct cccatgtcat ctgtctgtga acaatttgct tctttttcat aacaagcacc
     2341 acatccagta caatagttaa tatctttatc ttttaaaaat atcttctcca cttgattttt
     2401 agattctaat accccttgta taaactggtc acacaataag tcagaatttc caccttttct
     2461 aggactagct gatactatta atacattttt actcataaga tactgcctcc attttcttat
     2521 ttctactgct ctttaatact ttttctttct caataatacc ttcttcatat ctatcaatct
     2581 taaaatttaa tcgttccaaa gttttttgca tttcttctat tctttttgct agttggtttc
     2641 tctgctctat taaaagttct tttctagctt tgatagtact gctcccttgt tgaaacatag
     2701 ctacatactc aattaatgtg tcaatagaaa gacctgcacc tctcatacac tttatgaaat
     2761 taacccactc acaatcttct tctgtaaaag acctaattcc acttttattt ctatttacag
     2821 gtggtattaa accaattcgt tcataatatc gtaaagtatc tgctgaaagc tcgtattttt
     2881 tactaacttc tgaaatcgtc atacatatct tctcctcttg atattgtata gattgaatta
     2941 tttctcaaag gtttctgcta cattctttaa agcatctata attttaatgt gttgaggaca
     3001 gctttcttca cattgtttac attcaataca atctgaggcc tttccataag ttttagtata
     3061 attattatag tagaccatct gtgttgaaaa tccagtatta agagcttgtt tttctgcatt
     3121 gtacaacgca aaatagtttg gaatagcgat attttttgga cagccttcta cacaatactg
     3181 acaggcagta caaggaacta aaattgattc gttgataatc tctactgctt catctataac
     3241 attatattct tcttgcatga atggtttaaa gttttgcata tatcctgtat tatctaataa
     3301 ttgttccata tttgacattc cacttaagac catcataaca ttgtctttac ttgctgcgaa
     3361 acgtattgcc caagatggaa ttgacatatc tggattatag tcattgaaca acttctctgc
     3421 cttttctggt atttttgcaa gtgtacctcc ttttactggc tccataacaa ttacaggctt
     3481 attgtgtttc tctgctacct catagcactt tctagattgt atgctttcat tatcccaatc
     3541 taagtaatta atctgtaatt gcacaaagtc tacttctgga tgctcagtca agactttatc
     3601 taataactct gcactatcgt gaaaagaaaa accaatatcc tttatttttc cttctttttt
     3661 cttttcttct ataaacttaa agctatcaaa ttttttggct atttcataat gtgatacccc
     3721 tatgttgtgt aaaagatagt agtcaaaata gtctacacca catttttcta attgctcatt
     3781 aaaaatggtt tcttgctctt cttttttctt taaagccatt aatggaagtt ttgtagctat
     3841 agtaaaactc tctcttttgt gtctttttac tagagattct ctaagtgcta tctcactttt
     3901 tcccatgtga tacacatatg ccgtatcaaa atatgtaaat cccctttcta aaaaagtgtc
     3961 taccatttta tttaaatgtt ccatatcaat agttgtaaca tcattttcat cttttattgg
     4021 taaacgcatt aatccaaatc ctaatttttt tgcctccatt gttttacttc cccctttaaa
     4081 acttatttgt tttttctctc tactttaact attcttacaa gtatataatt cttgtataat
     4141 tatactttac tacttggagt taacttcaag tcaatagatt tttttatttt taagacaaat
     4201 attataaaaa tacagttctt taatattaat ccattacatg tttctttcac cctccaattc
     4261 tcaaacttca caaactccaa catgtaaaga aatatatact ttaagaaatt cagtccatta
     4321 atattactac aaatactttt tagatatgtt taaaaagaaa tttcacctaa aagatttaga
     4381 aaagatttag aaaagattta gaaataggtc ttgtgtctat tttaaaatat ataaaacagt
     4441 ggtaaagact tatttataag ataaaaaatg aatagtgtat acccttttta aaaagttttt
     4501 tcctttttat atttgtgata caatgttaaa tataaaaagg aaaattttgt attaaagaaa
     4561 tcttatttgt tgattttgta acttcaaact cagtatcaat ttgattttaa gattatattt
     4621 tattaagtat gctaaataac aaatttttaa agattgtgag gtaaaaatat gaataagaaa
     4681 aatttagcta tattaatatc aggtatgatg tttttttcta gttcttcagc aatttttgca
     4741 gacaatacta caaagcaaga aagattaata ggtaaaacaa gatatgaaac agcagtagaa
     4801 gtaagtaaat tgggatgggt tcaatcaaaa acagccatta tagttaatgg taattctatt
     4861 caaagtgcgc tttgtgcaaa tccatttgct aagctaaaga atgcacctat acttttagta
     4921 aacaacaaca gtattgaaaa ttcaactaag gctgagctta aaaggcttgg tgtagataat
     4981 gtgtacattg tagatagtgg aaatagcatt tcttctaaag tagaaaatga aataaaatca
     5041 cttaacataa aaataaataa aatagtagga aacaatatat atgaaatgtc tacaaatgta
     5101 cttaaagaaa tagataagat aaaaaagata gaaaatattg cagttgtaaa tggactaaag
     5161 ggactatctg atgcagttag tatagcttct ccagcagcaa tacataatat gcctataata
     5221 ttagtatcag atgaaaacag caatgatagt agtataaatt tcttaaacaa taggtctata
     5281 aaaaaatctt acatagttgg aggaaataag attttatcag acaatacagt aaagaaatat
     5341 caaaacagag aaagaatcag tggttcagat agaaacaata ctaatgcaaa aattattgat
     5401 aaattttata aagataaaaa aataaataat gtatttgtag ctaaagatgg tattaaccaa
     5461 gaaaatgcat tagtagattc attatcaata ggagtactag ctgctaaaga aaattctcct
     5521 ataatgatat ctaatggtag ccttgtaaat agccaaaaag aatttattaa aaataaagat
     5581 atagatgaaa taacacaagt aggaggagga tataatcaaa aagcatttga agatatttta
     5641 aaactaaaaa atgtgaacat aagaaataaa actaaaaaaa tatctttaat acaggctaaa
     5701 gaaatattaa aacaagctct tccaggaagc aatattttag tttgttatta tgatgaagat
     5761 gataatgaat atgactgcaa agttataaag gataatatcc tatatgagat agaaattgat
     5821 gcctactctg gtaaaataat agaaattgaa aaagatgatg acttaaatga taataattta
     5881 ggtacacaaa atatatcatt ggataaagct aagtctatag cattagatgc tattcctaat
     5941 ggtaatttaa tctattgtaa atatgatgct gaagataacg aatacgaggc taaagtaata
     6001 aaaaataaga aaacatatga attagagata agtgcatttg atggaagaat tgtagatatt
     6061 gacatggaca atgattctga tgatgacttt gatgataatg ataagaataa ctctaatact
     6121 aatgacaaca gttctattat tagtgctgaa aaagctaaat caataatgct agataaagta
     6181 cctggtggga aaatagtaaa atttaagtat gatgaagatg acaaagaata tgaaggtgaa
     6241 ataataaaag gaaaaactga atatgagata acaattaatg caattactgg taaaatatta
     6301 gaatttgaaa tagatgatta ataaaaaata tatattctat taattattac aagcaataaa
     6361 gccataaata ttgaaaattt caatatttat ggctttattt tatttttttt aatttaaatc
     6421 catattatgc cattatatat atctatgcac tcttttaaat taaaatcttg tccatcatcc
     6481 taaacctaat aaagttagat tttaaacagt atattgtgat ataagtaatt tgtgtagaat
     6541 tattgcgtga tttactcatg aataaacaat aaggttaata tataatttga ctatattaag
     6601 acgaaaggag acatatacaa tgaatacaat attaaaagta aataacataa ctaaaactta
     6661 taataaaggg caacagccga gtttagataa ggtatccttt aatgtattag aaggtgaatt
     6721 tataggtata atgggagctt ctggctctgg gaaaacaact ctacttaatg tattatctac
     6781 aattgataaa ccaacaagtg gagatatttt cataaataat ataaatttga aatcgcttaa
     6841 tgacacttgt gctgctgatt ttcgtaagga taatttaggc tttatctttc aagagtattt
     6901 tttactagat attcttacaa ttttcgagaa tatttcagta cctcttacac ttcagaaact
     6961 caacccagat aaaatagaaa acatggtaaa gagtctagct gaacgatttg acatagcttc
     7021 tcaattaaat aaatacccaa atgaattatc tggtgggcaa agacagagag cttcagcagt
     7081 tagagcaata gtaaaacatc ctagtattct ctttgcagat gaaccaacag gagctttgga
     7141 ttctaattca gctactgaac tactccactc actacaggaa gcaaatgagg agttgaaaac
     7201 aacaatttta atggtaacac atgatgccta tgcagcaagt tttgcaaata gaattttgat
     7261 ttttaaagat ggtcatatca ttaaagaatt aaataaacaa ccaaataata gacatacatt
     7321 ttatgagttg attattcaag aaattgcaaa attggatact aaaagatagg aggataattt
     7381 aatgaacatt ttttatatgg caataaagaa tcttaaaaaa aacttttcat tctattctct
     7441 atatctactt tcagtctctt ttgttattac tgtatttttc gcatttactt cattttctgt
     7501 taataagata atgctagaaa aaatttcaac tgatggtaga gttgaatcta tgtgtaatgt
     7561 tatttcgata tttttgatgg catttgtcat cttctatatg gcatactcaa atcgattctt
     7621 cttgagacgt cgtacaaaag aattaggtat atatgcttta cttggctatc gtaagtttac
     7681 aatattatcg ctacttacct ttgaaaatat attaatttgt agtggagctt ttattattgg
     7741 attggtatta ggagcattgg cacataaggg tattgtcttt ggaattacag cactacttaa
     7801 tttatcaatt gataattcac agataccatt ttttaatata aatgcaataa ataaaacaat
     7861 tatctttata gcattagttg ttttagtctt agctgtatca aatggtaaat ttctttataa
     7921 gacttcactt gtaaaattga taagatatga aaaaagtgca gaaaaaaaat taaagttcag
     7981 aaaaattcca gcctttatgg ggtttattat gattttaagt gggtattctt tatcactaga
     8041 tattcttcgt ggagctaagt cagtatggat aagtattgga ttttatcaaa tgggcttgat
     8101 aactatgatt atcattgtaa ttggtacagt tctctttatc aattcctttc ttccatatat
     8161 tatgcaaaag agtaaaaaaa ataaacgagc tttctataca ggtacaaaga taataactat
     8221 tccaaacttt atctatcgta tccgttcaaa ctctaaaact ctaattatgc ttacattact
     8281 ctctgctact actttgacag tttcaagtgt tatggcactg tccttatact accctattgc
     8341 atccgtttca cgtattgcac cttcagaaat agaatttaga gtagaaaaaa ataaacaaat
     8401 agaagatgta agacaaattg tcagtcaata tacgtctaat acagatgata ttatttttac
     8461 acagacaaat atttataagg taaaatcttc atcaaaagac cttccatttg aatatgaaat
     8521 tggtacatct aagaataatt tatctaatag tagttcttct aaagaagtta tttcaagaga
     8581 atcaggattt gaatgtatat ctttttctga ttattatact ttattaaagg cacaaaatcg
     8641 aacaaagcta ctaaaacaac ttaccccttt aagggataat gaatgtattt taatgaaata
     8701 tcaaccaaat aatgatggat atagtgaaaa tggaacgatt tatcaattac ttgttaatgg
     8761 taaagaaaca cctgtagctg taagggaaac atcattaaac aatgctattt cttttgccaa
     8821 tagcgttgga acacttatcg ttaatgatag tatatatgag caaatagcac aaaataaatt
     8881 accaatgaca agcatcttaa gtattaatgg taaatcaatt aaaaataatg aaaatttata
     8941 tagtaat
//
LOCUS       DAEMHW010000157         8781 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEMHW010000157 DAEMHW010000000
VERSION     DAEMHW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.157"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            199..549
                     /locus_tag="KPK67_002937"
     CDS             199..549
                     /locus_tag="KPK67_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG2459872.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            568..2211
                     /locus_tag="KPK67_002938"
     CDS             568..2211
                     /locus_tag="KPK67_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBG2459873.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2292..4349
                     /gene="recG"
                     /locus_tag="KPK67_002939"
     CDS             2292..4349
                     /gene="recG"
                     /locus_tag="KPK67_002939"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBG2459874.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4408..4962
                     /gene="rsmD"
                     /locus_tag="KPK67_002940"
     CDS             4408..4962
                     /gene="rsmD"
                     /locus_tag="KPK67_002940"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBG2459875.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLGFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4964..5461
                     /gene="coaD"
                     /locus_tag="KPK67_002941"
     CDS             4964..5461
                     /gene="coaD"
                     /locus_tag="KPK67_002941"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBG2459876.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5465..5989
                     /locus_tag="KPK67_002942"
     CDS             5465..5989
                     /locus_tag="KPK67_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459877.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6333..8252
                     /locus_tag="KPK67_002943"
     CDS             6333..8252
                     /locus_tag="KPK67_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG2459878.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHKSLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSIIELINKQN"
     gene            8265..8552
                     /locus_tag="KPK67_002944"
     CDS             8265..8552
                     /locus_tag="KPK67_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2459879.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     SDCDCIFTSQELLNELKGTCNVEVYPVKKYMDLAEVTEVVKTFLSDKNDKK"
BASE COUNT         3645 a          891 c         1625 g         2620 t
ORIGIN      
        1 atatatttgg aaaataatta ttatttttct ttgaatatat atactataga agaaatttta
       61 tccaaaaact tttctaggtt taaattatac catatttata gtatgatatg attataattt
      121 tatataataa ataagtaaaa atagtaaaaa ataaattaag aaatgttttt aataaaaata
      181 agcttaaggg ggtcaaccat gagtgcaaag gtaacgaatc aatatggaaa tatagaaata
      241 gataatcaag taatagctca agtcgtatat agagctgcaa tggaaagtta tggcttagta
      301 ggtttagcat ataagtctaa aggaatagta gagctattaa aaggcgagaa cgcaactaaa
      361 ggtgtaagag ttgaggaagt agaagaagat gccatagcaa tcgaacttta tgttataata
      421 caatatggta caaatatatc tactgttgcc aataatataa tagatagagt aaaatatgta
      481 gttgaaaaaa tgactggcgt aagagttact agaattgata ttaatgtaca aggaataaga
      541 gttaagtaat ttcataggag gaaacaaatg attcaatata tagatggtaa aagattaagg
      601 gagatgttta tttcaggtgc aaacaatctc caaaacaata aagagttagt tgacaagtta
      661 aatgtattcc ctgtaccaga tggagataca ggaactaata tgtcattaac aatatcttat
      721 gccttaaaag aattagcaaa agttgaaaat gataatatat cagatatcgg aaaagcatta
      781 tctaaaggtt ctttaatggg agctagagga aactcaggag ttatactttc acaaataatc
      841 agaggtatag ctaaatctat agaaggaaaa agtaaattat caacagaaga tttagcaaaa
      901 gcttttaaaa atggttcaga cacagcatat aaagctgtaa taaaaccaat tgaaggaact
      961 atacttactg tagttagaga aagtggagag tttgcaataa aaactgcgaa aaaagagaaa
     1021 gatgtagtaa agtttttaag tatgcttgtg aaggaatcaa attcttcatt agaaagaact
     1081 ccagaccttc taaagaatct aaaagaagct ggtgtggttg actctggtgg aaagggtctt
     1141 gtacttatat atgaaggaat gttagcttct ataaaaggcg ataacataga aataaaaaat
     1201 gttgacctag atactaatat ttcaacaagt atggattttg caaagagtac tactagcaca
     1261 gatgatataa aatattgcta ttgtacagaa tttattttag aaagttctaa ggtagaagat
     1321 actaaaataa gagacataat gatggcttat ggtgatagcc tagcagtagt tggagatgat
     1381 ggagtcataa aggtacatgt acacactaat gacccaggaa atgtacttca agaagctcta
     1441 aagtacggtc aattattaac tataaaaata gaaaatatga agttacaaca tgaaaataca
     1501 ttactggatg tagaagaaaa gaaagaaaat gatagtgaac ctttagaaga agaaaaagag
     1561 tttgggttta tagctacttc tatgggtgaa ggcttggcaa atatatttaa agatttcgga
     1621 gttgaccata taatagaagg tggtcaaact atgaatccaa gtactgaaga ttttatgaat
     1681 gctataaagc atataaatgc taaaaatata tttattttcc caaataacag caatataata
     1741 atggctgcta atcaggcaaa agaattgagt gataaaaata taatagttat acctacaaaa
     1801 aatacgcctc aaggatttgc tgcattagta acttttaatg gagaattaag cgaagatgaa
     1861 aataaagaag ctatgatgaa tgctttaaac tcagttaaat caggtcaagt aacttttgct
     1921 gttagggata cagtgatgaa tgaaattgat gttaaagaag gtaatataat tggaattgca
     1981 gaaggaaatt tattatcagc aggagattat gtggatgaag taacatcaaa ccttattgaa
     2041 aagttggttg atgaagatac tgctataata acattattct ttggtgaaga tgttacagaa
     2101 tctcaagcaa atgaactacg tacttcttta gaagaaaagt ttgaagatgt agatgtagag
     2161 ttatattatg gaggacaacc tctttattat tacttaattt cagttgaata atgattataa
     2221 gaacctgtat gaccaagtag cttttataaa attggttgta caggattttt gtgtagtagg
     2281 tgatatattt gttggattta tataaagatg ttcaatatgt taagggaata ggacctaaaa
     2341 aagcagataa attaaataaa ctgggtatat ttacactaaa agacctattg tattattttc
     2401 caagacaatt tgaagataga aataatctaa agaaaatagc acaattagaa gatggtgaga
     2461 aagttacgat aaaagcagta atatcaagta taaatacatt tagcccaaaa gaaggtatga
     2521 ctttaacaaa gattgatgta aaagatgaga caggctcggc taaattagtg ttttttaata
     2581 agagttatat aaaaaatact tttcgtccag gagattcaat attagttttt gggaaggtta
     2641 agaaaaaatt taataactta gaacttacat catgtgaatt agaatatctt actaattcac
     2701 ctaagaatac atgtagattt atgcctgtgt accagcttac atacggtgtt acaaataaag
     2761 aaattatgag tataattaga acagtacttg aagataaaga attaattata caagaatata
     2821 tgccacaaag aattatagaa aaatataggc tatgtagtat agattttgca gttagaaaca
     2881 tacattcacc aagtagtaag gaatcattaa aaatagcttt atatagaatt gtatttgaag
     2941 aattacttat actacaacta gggttgtttg tatttaaaag tggcagaaat aaagaagatg
     3001 gaataaagtt tgaaacaagt aaagacttaa aaaaaataat aagtgcttta ccatttaaac
     3061 tgactaaagc tcaaaacagg gcattagatg aaattataca agatatgaat ttagagaaaa
     3121 taatgaatag attggtacaa ggtgatgtag ggagtggtaa aacagtagta gctctcttag
     3181 cacttgcaaa ttgtgtttta aatggttatc agggcgcttt gatggcacct acagagatat
     3241 tagcagggca acattatatt tcacttacgg agtctctaaa agattttgga ataaatgtag
     3301 gtctattaat aggtagtctt acaaaaaaac aaaaggatac agtattggaa caaataaaga
     3361 ataatgagat tgatatatta ataggtacac atgcactaat tgaagataag gttgaattta
     3421 ataatattgg tttagttata acagatgaac agcatagatt tggtgttatg caaaggagca
     3481 aactttcatt gaaaggagca aatcctgata tattagtaat gacagcaaca ccaataccaa
     3541 gaactttagc acttatactc tatggagatt tagatatttc tattatagat gaacttcctc
     3601 caggaagaca gccaatagaa acaattgcta ttgaaaaaag taagcgagat agagcatata
     3661 ataaccttgt aagaagagag gttgaatcag gaagacaagt gtacattgta tgtcccttgg
     3721 tagaagaaag tgaagccata gaagccaaat cagcagttga acttgttgag gagttaagag
     3781 cggaatattt tcacgactta aggcttgggt tgcttcatgg taaaatgaag tcaagtgaaa
     3841 aagatgaagt aatgagactt tttaaaaata aagaaataga tattttagtt tcaacaacag
     3901 taatagaagt tggtgtcaat gtgccaaatg caacattaat gataatagaa aatgcagaaa
     3961 gatttggact tgcacaatta catcagctta gagggcgagt tggaagaggt agtcataaat
     4021 cgtattgtgt actaatttat gattctaaaa cagatgtatg tagacaaaga atggctataa
     4081 tggaagagac caatgatggt tttaagattt cagagaagga tttagaaata agaggacctg
     4141 gggaattttt tgggacaagg caacatggtc ttccagaact taaagtagca aacttgttta
     4201 agcatataaa aatactgaag ttagcacagc aagaagctcg atatatattg ggtgaagata
     4261 ataatctaca attaaaagaa aacatggcac tcaaaaaaga aattatagat aaatttaaag
     4321 atacattgaa agaaatttca ttaaattaat tttaaattgt gttaaaatat tactaatgta
     4381 ataatgtgct gaagggagat tgtcatattg agagtaattt caggaaaagc gagaggatta
     4441 aaactaaata ctccaaaaaa tgaagatgta aggccaacta cagatagagt aaaagagtct
     4501 ctatttaata tgataaattc atatataatg gaaagtgaag ttctggattt atttgctggg
     4561 actggttctt taggaataga gtgtttatct aggggtgcca agtcatgtac ttttgtagat
     4621 atcagtaagg aaagcataga tattgttaag tccaatataa aaaaggcaag agttgaaagt
     4681 gaaagtatta ttttaaacct tggttttaag actgcaatag ataaacttaa attacaaaat
     4741 agtaaatttg acataatttt tatggaccct ccgtattata aaaatatgtt tatagaagct
     4801 attgaaaaaa ttgataattc taatctattg aacgaagatg gaataatagt tgtagaacat
     4861 gatacgaatg atttatttcc agataagata tgtaaattgg agaaaactag agataagaaa
     4921 tatggaaata ctacattaac tttttataag atggaggcat aatatggaaa ataaacctag
     4981 aaaagctata tttgcaggaa gttttgaccc aattactaat ggacatttag atattatttg
     5041 cagggcttcg aagttatttg acgaattaca aataggagta ttaaataatc ctaataaaaa
     5101 gggtctattt agctttgatg aaagagtaaa acttatagaa aaaagtacaa gccatttaaa
     5161 taatataaaa gttgtgactt ttgatggttt attaataaat tactgtcagg aaaatgggat
     5221 tggagcatta gttagaggtg tgagaagtgg tgctgatgtt gattatgaac ttcagatggc
     5281 gcatatgaat agagagctta atccagatat agagactata attttaccaa gttgtacaaa
     5341 atattcattt ataagttcat ctttaataaa agaggttttg ctctttgatg ctgatataaa
     5401 aaatttggtt ccaaagattg ttttggaaga attaaagaaa aaaactagtg ggggtaattg
     5461 atatatgaag atagatttag aaatgattga actatttgag cagttagagg agatatttag
     5521 aagtgcttct acaatccctt tctcacataa atgcacagtg gataaagatg aggttttagc
     5581 aataattaat gatataagaa ctttaatacc agaagaagta acacaggcag tatggattaa
     5641 taaagaaaga aataaaataa taagtcaagc taagcaagat gctacaaata tagttgaaca
     5701 agcgaaaaaa gaagctgaga gaatacaaca agaatatcaa gataatatag aggaattaaa
     5761 gaaaaattct gaagatgtag taaaggcata tgtagaatcc agcgaacctg ttgttcaagc
     5821 agaccaaaga gcaaaagata tagttgatag agctgagaga atagccaatg agataagaat
     5881 tgggtcaatt gaatatgcag aggatgtgct ttctagtgtt gagtataatt taagagaaat
     5941 attagaagaa gtaaaaagag ataaagctga acttagacct aaaaaataaa ttttaaataa
     6001 ataaaaatgg agctatcaca caaaaaattc caattgatgt gtgatagctc tcttttaata
     6061 tagcaatttt ttgttatata attgtgcttt ttagattact tatatcttat tttgatatta
     6121 taatgtagtt taatctttta tttgttgaaa taaacttatt tatttgtata aataatatag
     6181 gttattttca aattttttta ataattttac cactgtcttc ttttagatta gagttagact
     6241 gtgcaaaatt ataaaataat actaaatcat gtgcaattat ttggtatggc actacatgta
     6301 gaaaatatag ataataaaat taaggaggta gtatgaagga aagggctaaa aaaatattag
     6361 attttattat taaaaataac aatgtaacat cacaagaact acaagaaaag tttaatgttt
     6421 caaagagaac tatttattac gatattctag ctataaatga acaactagga aaaagtggta
     6481 atataaaaaa tgtaaaacac aaatttatat ttgaaggtaa tttatgtgat gcaaggaaaa
     6541 taatttcaac tgaagaagat aaatttttag atagtgatta cagaaaaact tttatattaa
     6601 acaagatatt acttggcgaa aaaatatcaa tagaaaaact aacaaatgaa atgttacttt
     6661 caaaaaacac agtagtacaa acaataactg atgtaaaaaa ataccttcaa acaatggggt
     6721 taagattaga gtataaggga aagtacaaaa ttataggtga tgaatacgtt attagagagc
     6781 tgtttctgat aattgttcaa gaaaatgtac tggaaattaa ttctataagt gaggaagtaa
     6841 gtagttttga tacaaaagga catataaaac ttactgatta ttctttatta aatctaacta
     6901 agtttgtgga atttctaaat aaaaggataa gagatgggaa aacattatac tcatataaat
     6961 atctaaatga agctaaaaaa attagttact ttagtaattg taaagaactt ttatgtgaag
     7021 aagctaatga aaatgaacaa gcctatatat gcacttacat aagttcactt cccagcttaa
     7081 atagtgaggt taaggaagat gttgttgaag aatatgtaga taaacttata gataagtttg
     7141 aagtaaatac agcaataaaa ttagaaagta agcatgagtt taagaaaaat attttaaggc
     7201 acctccactc ttcttacaac agaatacgat ttaagtttcc tattagaaat ccaatgttag
     7261 atgagactaa gtataaacat aagtcacttt ataaaattat aaaatcaatt atagaaaatg
     7321 aagaagagtt tcctgtattt gaggggataa gagaagaaga gataggattt atagcagcgt
     7381 attttggagg gtatttaaga gggtctaggg ataatggtct aagaagaaat aaagtcttac
     7441 tagtttgtcc taatggtctt atggtatcaa agagtttgga aatacaacta tataaataca
     7501 taccaaccat agaaatagta ggtatagtgt ccattaagca actaaaagaa gtaaatgtat
     7561 actacgatta cataattaca actatagata tacaaaatgt aaacaatgta attgttgtaa
     7621 atcctctttt aacatcatca gatgttcaat tacttatgaa caaactgata agtgtaaaag
     7681 aaaacgagaa gtatttcaat cttgaattaa taatacaagc tataagaaaa aatggagtta
     7741 tcaataatga agaagcacta aaagctgatt tattaaacat aatacacaag atagatgaag
     7801 gagagatgta tcaaccaatg ttaaaggaat taattaatgc agaaagggtc aatataataa
     7861 aaagtgttag agattggaaa gaagcaataa aaatagcctc aaagccattg ttagaagata
     7921 actctataga agagttatat atagaaaata tgataaagtc agttgaaaaa tatggaccat
     7981 atattgtttt agctgataga tttgctctac cacatgcaag ttctaaagaa ggagtaaaca
     8041 agcttgctat gtctttacta atagtagaag atgaagttga tttattagga aaaccagtaa
     8101 atatatttat ggtattagct gctgtagata acactactca cattagagca ttagcatcac
     8161 tatcagagat gatgtacgag gaagaaaatg taaaactaat aattaatggt gataaaagtt
     8221 caataataga acttataaat aagcaaaatt aggagggatg aaaaatgaag atattagcag
     8281 tatgtggttt tggtgtaggt tcatcaatgg tattaaaaat gacaatacaa aaggctctaa
     8341 agcagttagg tatagaatgt gaagtagaaa atacagatat aaattcagct agaggttctg
     8401 actgtgactg tatattcaca agtcaagaat tgttaaatga attaaaagga acttgtaatg
     8461 tagaggtata tccagttaaa aagtacatgg atttagcaga agtaacagaa gttgttaaaa
     8521 cttttttaag tgataaaaat gataagaaat agtataagtt ttaaaaagta aatttaagtt
     8581 aatcagaata aaataaaaac ttactaaatt caaaaatagt aagaaagaga ggagggattt
     8641 agtggtagaa aacttaaaca gttgacctat cactaaaaaa tataatgctt gattaaaaaa
     8701 tagctacgca cacttgtgac tttagtcatg agttagtagc taaattagtc agcatatagg
     8761 gaaacctata tgtagagata g
//
LOCUS       DAEMHW010000158         8716 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEMHW010000158 DAEMHW010000000
VERSION     DAEMHW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.158"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1300
                     /locus_tag="KPK67_002945"
     CDS             <1..1300
                     /locus_tag="KPK67_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG2459880.1"
                     /translation="KEDYLRKIAYTDPLTGADSIDKFKINSNKLFAKNNPEEYALFYI
                     DVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFVLLIKYKTDDDI
                     KTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRANTARKTIKGGH
                     KNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDYQIIGAEALVRW
                     DNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQKVVPISVNLSRM
                     HFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNNLKEKGFVISMD
                     DFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMAKELKMKVISEG
                     VETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            1620..1832
                     /locus_tag="KPK67_002946"
     CDS             1620..1832
                     /locus_tag="KPK67_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2459881.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            1845..2066
                     /locus_tag="KPK67_002947"
     CDS             1845..2066
                     /locus_tag="KPK67_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2459882.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            2166..4322
                     /gene="feoB"
                     /locus_tag="KPK67_002948"
     CDS             2166..4322
                     /gene="feoB"
                     /locus_tag="KPK67_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG2459883.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            4348..4500
                     /locus_tag="KPK67_002949"
     CDS             4348..4500
                     /locus_tag="KPK67_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBG2459884.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            4680..5390
                     /locus_tag="KPK67_002950"
     CDS             4680..5390
                     /locus_tag="KPK67_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG2459885.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            5475..6245
                     /locus_tag="KPK67_002951"
     CDS             5475..6245
                     /locus_tag="KPK67_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2459886.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            6238..6969
                     /locus_tag="KPK67_002952"
     CDS             6238..6969
                     /locus_tag="KPK67_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459887.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            7003..7962
                     /locus_tag="KPK67_002953"
     CDS             7003..7962
                     /locus_tag="KPK67_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2459888.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSLLEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKQINIKDMLYNQN"
     gene            complement(8060..8656)
                     /locus_tag="KPK67_002954"
     CDS             complement(8060..8656)
                     /locus_tag="KPK67_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBG2459889.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT         3317 a          927 c         1608 g         2864 t
ORIGIN      
        1 gaaagaggat tatctaagga agatagcata tacagaccca ttgacaggag cagatagtat
       61 agataaattt aaaataaatt ctaataaact gtttgctaaa aataatccag aagaatatgc
      121 gttgttttat atagatgttg acaaatttaa atatataaat gatatgtttg gatatgatat
      181 gggaaatgat actttgatac atatatcaaa tactattgca agtgaattaa aggaagatga
      241 aatttttgca agagtttctg ctgaccattt tgttcttctt attaagtata agacagatga
      301 tgatattaaa acaaggttaa acaatattta caataaggtt caaatactta gtaatcctaa
      361 aataaactat tataaattga ttttagattg tggtatatat aaaataagta aaagtgataa
      421 tgacataaat actattatgg atagagctaa tactgcaaga aaaactataa aaggtggtca
      481 taaaaattca tttgcttttt acgataagga aatgcacaaa aaaatattaa aagagaaaga
      541 aattgaaaat tcaatggtag atgctcttaa taatggagaa tttattgttt attttcaacc
      601 taaatatagt ctttcagatt atcaaattat aggggcagag gctcttgttc gttgggataa
      661 tccacaaaaa ggtctaatac caccaataga atttattcct gtatttgaga ggaatggttt
      721 tattgtaaat attgatttct atgtatttga ggaagtgtgt aaaaagatta gagaatggat
      781 ggatgaagga caaaaagtag tacctatatc tgtaaacttg tctcgaatgc attttgtaaa
      841 tagtaatttt atagagaagt ttaaattaat tgtagataaa tacaaaattc cgacaagact
      901 tatagaatta gagctaacag aaacagcagt attagataat atagaaggat tacttgatac
      961 aatgaacaat ttgaaagaaa agggatttgt aatctctatg gacgattttg gtacaggata
     1021 ttcctctctt aatcttttaa aagaattgcc tgttgacata ttaaaactag atagagcatt
     1081 ttttacagaa aaagatgaga gtaataatga aaaaattgtt atatctaatg tcattaaaat
     1141 ggcaaaggaa ctgaaaatga aagtaatatc agaaggtgta gagactattt cgcaagtaga
     1201 atttctaaag caaattgggt gtgatatggt acaaggatat ttgttttcta agccaatgcc
     1261 tgtaaaagag tttgaaaaaa tcgcttttaa aaaagaataa agcaatgtgt aaataatgac
     1321 atatataata aaggtgcttt aatattagtt gagtatacta ttttagagca tctttatttt
     1381 tgtattaatt tttacgattt tattaataaa ataaatcaaa tgagaaaaaa atcaaaagaa
     1441 tacaataatc acttgcaaaa aatatactat tgtgatagac ttaaatcaaa attaggaatt
     1501 gtaaaaagaa ggaaaaaata ggatattatt ttaatagatt aagatttaag gtattgtaat
     1561 ttatatataa gataacgatt gcttgtagat ttaaattact ttaagaggag ggatattata
     1621 tgatgccatt aatattatca aatacaggtg aagaagtagt tataaagaaa atttcgggaa
     1681 gtgataagac gaggactttt ttaaatagta taggttttgt agtgggtaca aatgttaaaa
     1741 tagtttctaa gatagatggt aacttaataa tagacgtaaa agatactaga gttgcattag
     1801 ataaaaaaat ggcaagtaga ataataatat agggaggtaa tgacatgaac agactgaagg
     1861 atattaagtg tggagagact gtaaaagtca aaaagttaga aggagaagga gctacacgaa
     1921 gacgtataat ggatatgggg attacaagag gtgttaatat atttataaga aaagtagctc
     1981 ctcttggtga cccaattgag gtaacagtca gagattatga actatcaatt cgtaagtcag
     2041 atgcagaaaa aattattgtg gaataataaa gattaattag agtagggtta atgtaattat
     2101 ataatgagaa tgattttctg tatcacaaac agtgatgtat ttaaataata atggagggaa
     2161 tggaaatgtc aataaaaata ggtctcatag gaaatcctaa ctgtggtaag acaacaatgt
     2221 tcaatgggct tacaggctca tcacaatatg ttggtaactg gccaggagta acagttgaaa
     2281 aaaaaggtgg aaaattaaaa ggaaataaag atgtggaaat agtagattta ccaggtattt
     2341 attctttatc tccatataca ttagaagaag tagttacacg taattttatg ctagatgata
     2401 agccggatgc agtaataaat attgtagatg catcaaatat agagagaaat ttatatctta
     2461 caactcaagt tttagagcta ggtataccga ccgttatagc tcttaatatg atggatattg
     2521 taaataagaa tggtgataag ataaatatta aagaactatc tgaagttata ggttgcccag
     2581 tggtagaagt aacagcagtt aaaggtcaag gaattatgga ggctgctgaa aaggcagttg
     2641 aacttgcaag tagtaacaat aaattaaatt tcaaattacc ctttgttgat gaaagtaagg
     2701 atgcaataga aaaaattgaa aaaataatag aagaaaagac tccatatata gatgtagaaa
     2761 cacgctggtt agctataaag ctatttgaga gagatgaaaa tgttatacaa aaacttgata
     2821 tttctaaaac aatattgaat agtattgaag aaataactag aaattgtgaa gatgaattgg
     2881 atgatgatag tgaaagtata ataacagcca atcgttatga atttattagt tcaataattt
     2941 caagtataat aaagaagaat cgaaaaggta aggaaactgt atcagataag atagataaga
     3001 ttgtaacaaa tcgtatacta gcattgccta tttttgcact tataatgtgg ggagtttact
     3061 atatagcagt aagttcattg ggtactatag caactgattg gacaaatgat gtactgtttg
     3121 gagaaattat tcaaggcaat gtaagtaatt ttctagcttc tttaaatgta gcagagtggt
     3181 tacaaggact tgtagttgat ggcctaattg gtggagttgg agcagtacta ggatttgtac
     3241 cacaaatcat gcttttattc ttattattat caatattaga agattgtggg tacatgtcac
     3301 gtgtagcttt tataatggat agaattttcc gtaagtttgg tctttcagga aaatcattta
     3361 taccaatgct tataagttca ggttgtggag ttccaggagt tatgtcaact cgtactatag
     3421 agaatgatag agatagaaaa atgactatta tgttaactac attcattcca tgtggagcta
     3481 agattccaat aatagcatta tttgcagggg cattatttgg aggtgcttca tgggtagctc
     3541 catcaatgta tttcttaggt attgcaatga taatcatttg tggaattata ttaaagaaaa
     3601 ctagtttatt tgcaggagaa ccatctccat ttgttatgga attacctcaa tatcatattc
     3661 caagtgcaaa aggtgtttta atacatatgt gggatagagg aaaagcattt ataatcaaag
     3721 caggaacaat tatatttgtt gcttgtggag taatctggtt cttacaatca tttaattggt
     3781 cattacaaat ggtagatgct ggagatagta tattagctag cttaggaaat atagttgcac
     3841 caatatttgc tcctcttgga tttggaaatt ggcaatcatc agtagcaaca gttacaggtc
     3901 tagtagctaa ggaaaatgta gttggaacat ttggagtttt atttggtata tcagatgcaa
     3961 cagagcaaga cccaacactt cttgcttcag ttgcaagtat gtttacagta gctagtgcgt
     4021 ttgcatttat ggcatttaat atgctttgtg caccttgttt tgcagctatt ggagccataa
     4081 aaagagaaat gggttcatgg aaatggacat ggattacatt aggattccaa actttaacag
     4141 catatataat tgctcttctt ataaatcaag ttggaagttt agttttaggt actggaggaa
     4201 gtattgctgg ggcaataata tctatattta tagcagtagc agtagtcttt gtagttttaa
     4261 cttattcaaa taaaaatatg aaaaaagaaa aaatgggtaa attaagttat atgaaaaatt
     4321 aattcatata aataaggagt gatgaatatg gctacattta ttatagcagc tattgtagta
     4381 gtattaatgg cactagctgt aatgtatatg gttaaaaatt caaaaaagaa tggtagcagt
     4441 tgtggatgtg gttgttctgg atgctcaagt agtaagagtt gtcatagcgc taagagataa
     4501 ataggataag agcctgataa aaagttaaat tgctttatta tcaggttctt attttttgta
     4561 taaataacaa atttcatatt tgaagtaggt tagactatgc tgtaatttta ctgtataatt
     4621 atatataaat atatgattag gagggattat tgctaaatta agcaatataa gtgaatatta
     4681 tgtttaaatt tgatgttaca aacgagctta tgtgtatagc aaggggaagt ggtaaatttt
     4741 ttgcaaaaaa aggtgctatg gtagccttta aaggaaattt taattttgaa aaattgctgt
     4801 taggtccaag taatggcggt ggattgggaa gagcattatt aggtcatgtt cagaggtcat
     4861 tgacaggaga acaaatgcca ataatggtgg ttgaaggtga aggtgaaatt tacctagctc
     4921 aaaatgctta ccatgtagat gtaatatcaa ttgacccagg tgattccatt tatgttgaga
     4981 gtgaaaactt attggcattt tctgaacaat taaattacag tgtaaaattg attggttcag
     5041 gggtaatatc tcaaaaaggt ctttttacta cacatttagt aaataaaaca ggtcaaaatc
     5101 aagatgttgc tataataaca gatggtaatc ctctaattct tgaaggacct tgttgtgttg
     5161 acccagatgc attagtagct tggactggaa gagaaccata tcctaaagta tcaaaactat
     5221 catggaagac ttttattggt cagacttcag gagaatctta tcatatggaa tttgttgaac
     5281 ctggacaaat agtcattata caaccatctg aaagattgtc tggcttaaaa acagattcta
     5341 caccttctga tagaatgagt tcaggtggat taagacttgg catagattaa atcttatgat
     5401 atagcttaat tattatttaa gatattaaaa aataaaaagg gtagcaggga tagatatgtt
     5461 aaaaggagtt ttatatgttt gagaaaattc ataaaaggct taaaggatat tatatttttt
     5521 taataatttt aattttatgg aagatagtat gtgttttggg tatatggagt agctatattc
     5581 tgcctcctcc tgaaatagtc ttagatactt ttataaaaat gatatatgac ggttctattt
     5641 tcttaaatgt ttatgccagt gtaagaagga tattaatagg ttttttaata agttcattga
     5701 tggctatacc acttggcgtt gtatttggag taaataaaaa aatgtatgaa tatttcaaac
     5761 cactatttga atttatgcga agtacacctc cactagcttt ggtgccaatg cttattttat
     5821 ggtttggtat aggtgaagaa tctaaaatta taattattat tttggcatcc ttttttccaa
     5881 tatttttaaa tacattgaaa ggtataaaaa attgtgacaa taaattaata gaagtaggta
     5941 aatcttttaa tttatctaaa aagagaattt tttataaaat aataatccct aattctatgc
     6001 tggatattgt tgttggactt agtttagggc ttgggtatag ttttagggct ataattggag
     6061 cagagcttat tgcagcctct tcaggactag ggtatttgat ttctgatgga aaagatatgt
     6121 ctagaactga tgttgtattg gtaggaattt ttgttattgg atttttaggt atactttcag
     6181 attatctatt ttcaaagata ataacaaaat ttagcaaagg gaagcaggtt gatttaaatg
     6241 gttagaagtg gatttttgct agagaatata tataagaaat atttagttga taataaagag
     6301 catttagttc ttgataatat atctttaaat atatctagtg aagagataac tgttatatta
     6361 ggtgaaagtg gatgtgggaa aacaacgcta cttagaattt tagcagggct tgaaaatgct
     6421 actagtggaa acatatattt ttttaataat gataaaaagt gtactccaaa agtaggaatg
     6481 gtatttcaag aaagtagact tatgccatgg ctgaatgtaa gtgaaaatat acttttacat
     6541 actgaaaaag ataatagaaa taaagttgat ttagacaagt atttaaaaat gatgaagctt
     6601 gaaaagttta aaaactcata tccaaatgaa ttatcaggag gaatggctca cagagtaagt
     6661 atagcaagag cactttcctt caatcccgat attttgctga tggacgaacc atttgcagca
     6721 ctagattact tcacaagaag aaaaatgcaa aaagaagttg taaatatcca taaaaatact
     6781 aaaaaaggtg tagtatttgt aactcacaat attgaagaag ctatggaaat agcaaagaaa
     6841 ataattgttt ttagtaaaaa taaaaggatt aaacaattta gtgtcgaaga tgaatataat
     6901 agagatttaa ctaaaaacta ttatataaac ttgaaaaaag aaatattaag ggaattgggg
     6961 gagttttaat tgattaaaag aaacaaaaag attttagcag taatgacatc tttaataatg
     7021 gtgttggttg ggactgtagg atgttcaaca acaaatgata agaaagatga acctgcaaaa
     7081 aaagaggcta gtttacctaa ggaaataaat ttaacttatg taaaatcacc acttaatgtt
     7141 cctagtctat tagaaaaaaa ggatgattta tttggaaagg aatttaaaaa ggacaatata
     7201 aaagtcaatt tccatgaatt gacaacagga ccagagcaaa ctcaagcatt agcagcaggt
     7261 gagctagact ttttacatgc attaggagga acgtctgcaa taatagctgc atcaaatggt
     7321 gttggtctta aaattacgaa tatttatagc cgttcaccaa aaggatatat gcttataact
     7381 aataatgata atattaaatc tataaaagac ttaaaaggta aaaaaattgt aggtccaaaa
     7441 gggactatac ttcatcaact gttgattgct gcacttgcta aagaaggcta tactatagat
     7501 gatgttgaat atgtaaatat ggatattcct agtgctgctt ctgcattaga aagtaaaagt
     7561 gctgatgtag caatgcttgc aggtccagtg gcattgaaaa cgataaattc aggtgccaaa
     7621 atgattgtaa atggagaagg attagtaagt ggaataatag ttactgctgt aagtgatgac
     7681 tttgctgaaa aatatcctga attagtaaaa agatttatga aggttcatga agaaacttta
     7741 aaatatatga atgaaaacaa ggatgaagtg atggatgttg tatctaaaga agttggttta
     7801 tctttagatg aaacaaaaga aatgtattct tggtatgact ttagttctaa aataacggac
     7861 aaggatataa aagaattaga agatacacag gaatttttaa tgtctaatgg aatgcaacaa
     7921 aagcaaataa atattaaaga tatgttgtac aatcaaaatt agaattcttt aaaatattaa
     7981 agtggaaaat acacccttat ttattatgga aactatatgg taaaagaggg tgtattttta
     8041 ttattataca aaaatacaat taaaaacagc ttactatttt atctacactt atgtagtcta
     8101 gtaattttct cattttctta cggtgaagta aaaacttcga atatgtgtcc tcggattgtt
     8161 ttgtgtcacc ataaactttc caatatttaa tcatagaaac ttcataatta ctatatccca
     8221 taaatccaat cacatcttta agaggatatc ctaaaaaaat acctatttca tcaggaaaaa
     8281 catcactctt aagtttatct actaaatgct ctaaataagc attaacattc gtattttgtt
     8341 tgtatcctaa aaactttaaa aagtttactg tttttttaca ttttaatttt tctgataatg
     8401 cttttttatt tacaaataag atttttaatc cctttttttc tttatcaata actataaaat
     8461 ctattttctt acatttatat agatatttat aaatatcatt aattttttcc tctctattta
     8521 catcagtaaa cgaaatattt attatttcag caggtttaga gcctaaaaag actggaccta
     8581 aaacttctaa aagccatttt atatatgatg attcaatttt atttttacaa caaaattgtg
     8641 aattacaaca agacataaaa gataacctcc ttcaaatgaa aattaatatc aagtaattag
     8701 attatactat gaatga
//
LOCUS       DAEMHW010000159         8649 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEMHW010000159 DAEMHW010000000
VERSION     DAEMHW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.159"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            29..1309
                     /locus_tag="KPK67_002955"
     CDS             29..1309
                     /locus_tag="KPK67_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBG2459890.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNLAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1572..2963)
                     /locus_tag="KPK67_002956"
     CDS             complement(1572..2963)
                     /locus_tag="KPK67_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG2459891.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(2974..4194)
                     /locus_tag="KPK67_002957"
     CDS             complement(2974..4194)
                     /locus_tag="KPK67_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBG2459892.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGVFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4208..4996)
                     /locus_tag="KPK67_002958"
     CDS             complement(4208..4996)
                     /locus_tag="KPK67_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG2459893.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(5013..6287)
                     /gene="larA"
                     /locus_tag="KPK67_002959"
     CDS             complement(5013..6287)
                     /gene="larA"
                     /locus_tag="KPK67_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBG2459894.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(6405..7118)
                     /locus_tag="KPK67_002960"
     CDS             complement(6405..7118)
                     /locus_tag="KPK67_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG2459895.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7243..8502
                     /locus_tag="KPK67_002961"
     CDS             7243..8502
                     /locus_tag="KPK67_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBG2459896.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
BASE COUNT         2780 a         1485 c          988 g         3396 t
ORIGIN      
        1 aaataattaa agataaaagg agtattttat gaacatactt ggattaatag ttgaatataa
       61 cccatttcat aatggacaca tatatcattt atctaaatct atagaaaaaa caaatgcaac
      121 acatactgta gcaataatga gtggaaattt tctacaaaga ggcgaaccag ctttatttga
      181 taaatacact agagcaaaaa ttgctgtcga aaatggtgta gacttagtta tagaattacc
      241 aactttattt gcttgtcaaa gtgctgaaat tttttctcat ggtgctataa caattttaaa
      301 ttcactaaac tgcataaatt ctatatgttt tggtagtgaa gaaggaaata cagaaattct
      361 ttataatcta gctgaaattt tagcaaatga accaaatgaa tttaagttac ttttaaaaaa
      421 atatttagat gatggtctgt tatttccaac agctagagct aatgcacttt ttgattatat
      481 acatcaagac aaaaaaaata attttataca aaattattca aaggaacaat tatcaaaaac
      541 attaaattca tcaaacaaca tactgggtat agaatacata aaaaacctca tccatttaaa
      601 cagtagtata aaaccattta ctatctccag ggtccaatca gaatataatt ccgaagaaat
      661 tgagagtgat atttgttctg ctactgctat aagaacttca cttaaagaaa atacagacct
      721 ttttcattta aaaaatgtta ttccagaaaa aacttataac atcctaaatg aaaaaataga
      781 taatggtttt ttacctatgt ttgatgactt cttctttgat attttaaaaa caattatact
      841 tagagatatg gacactttaa aagagtattt tgaagtaaat gaaggcatag aaaacagaat
      901 ctataaagat atctttacat ctgaatttct acatgaacta caattagcaa ttaaatccaa
      961 gcgatacact ttaaccaaaa tcaaacgaac tctaaacaat atattgttag gtattaaaaa
     1021 aagcgacatg caacttataa aaaacataga tactatacct tatataagaa tacttgcttt
     1081 taatgataaa ggtagagaaa ttctaaaaga gataaaaaat aaatctgaaa taaaaataat
     1141 aaacaagttt tcaaatatat cattttcatt agatgatacg atttttaaaa cactcataag
     1201 ttatgatata aaagctacaa atatatataa ttcaatatat tataaaaaca ataaagattt
     1261 agtaaaaggc cctatggatt tttatactca tccaatatat gtaaagtagt tttttaaaat
     1321 aactatattt ataaaaatca ttttattaac tctttattat ataagataca atagtcttac
     1381 tttatttatg tacacatgct tttaggatga caacatcaac ttaaatatta tgttactttc
     1441 taaatttaag taaatattgc catcctaaaa attttacaat ttagcttcag atttaaaagt
     1501 aaagtttaaa atattatatt taaatctaat ttaatatttt ttgttttgta aatattatta
     1561 aattaatatt tctaatatat tttccctgga tttaaaatat ttttagggtc aaaagctaac
     1621 tttatatttt taattagcat catataagca tctgaatttg attcatgtaa gtactctttt
     1681 ttagcatatc ctatgccatg ttcaccagaa acttgaccac taagttctct agatttttta
     1741 tacatatagt caaaagtttc ttttaatctt attttccatg tattatcatc cataccatct
     1801 tttaaaatat atatgtgtag atttccatct cctgcatgac caaaactttt tattcttatt
     1861 tttaatttgt cttgaagctc gtgagtgtat cttataaact ctgctatctt atctcttgga
     1921 acaacgacat cacattcatc catttgtgtc gtagatgcct ttatagcttc taaaaatgca
     1981 cctctggctg accatataga atcgtttctt tcttgtgtat ctgatataaa tacatctaat
     2041 gcaccatttt ctaaacataa attagctact ttttcatatt ctttttctat atcttcagtg
     2101 ctatttccat caaatgtaag taaaagataa gcatctgatg tattatctgg aaacttttta
     2161 cctaaaaatt cttctgctgc caatattaca tctctttcca taaattctat agccgttggt
     2221 attgattttg attttattat tttaggaaca gtttctattg ccattgataa atctggaaat
     2281 ggtataagca aacttatact tttctttggt aaaggaagca attttaatat agctttagta
     2341 actattccta aagtaccttc tgagcccact aataaatcct ttatactata tccagaacta
     2401 tttttaacca cctttccacc tacatttata atttctccat ttggtagtac aacttcaaga
     2461 cctcttacat aatcccttgt aacgccatat tttactgctc tcataccacc tgcattagtg
     2521 tttatattac ctgcaattgt agcacttttt tcacctgggt caggtgggta aaataaatct
     2581 ctatcttgaa catattgacc tattgtcatc aataaaacac ctggttctac tgtcaatgtt
     2641 aaattttcat aatcaacttc aagtatttta ttcattttac atagattaat cattattccg
     2701 ccatttatgg caacagcagc acctacaaga ccagtacctt gacctcttgg tgtgactggt
     2761 atattatttt tatatgcgta tttcattatt ttagatactt gttcagtttc aagtacattt
     2821 actaaaactt cagggtactt ctcaatccct ccaagttcat catgtgaaaa atcttcattt
     2881 atatcacttc caactaatac attttcttct ccacaagtat ctattaaaaa atctatatct
     2941 tttttatcga ttaatttata catttctatc cccctattta gctgctgatg ccatatattt
     3001 ttgattatta tctttgttat tttctatttt ttcaatcaat ttaggaataa tatcataaat
     3061 atcacctatt aaagctagat gtgctacatc aagtataggt gctttatcat ctttatttat
     3121 tgctactata tagtcagcac cctgcatccc tgcaacaaat tgaacagctc cagatacacc
     3181 acaagttatt atcaatttag gttttacagt tctaccacta agacctattt gttttttagc
     3241 atctatccaa cctgcttcta ttacaggtct tgttcctgcc acttgagcgt ttaacttatc
     3301 agctaattta tatatcatct ccatatcttc ttgttttttt attgctctgc ttgccacaac
     3361 tatcacttca gcttcttcca gaccaacttc tttatttttt tcttttacac tcagtacctt
     3421 aattttagat tttagttctt ttttttctac tttacataaa gttatctttc ctgtagtatt
     3481 ctctattttt tctggtgctg aaaatatttt atatcttaca gtggcaaatt gtggtctatt
     3541 attaggtgtg tttatatgag ccattatatt tccaccaaat gctggtctta tttggtctaa
     3601 atcagtattt gattgaatat caagtattgt acaatctgct gtaagacctg ttctaaatct
     3661 agcggcaagt cttggagcta atgacctacc taatgtagtt ccaccaacta atactatcgt
     3721 tggttttatt ttatctataa agttttctat tgcttttgaa tatggttcta tcctaaaatc
     3781 tttaaattca tcttcatcat atacaaatac ttcatccacc ccataagaaa tcaaattact
     3841 gcacttatct tttatgtctt ttcctacaaa tacacaatac acaggttgtt ttatttttga
     3901 tgccatctca cgagctttac ctataagctc gtacgttaca ggatgtatat ttccattatg
     3961 atgttcaaca tatactgcaa ttcccctcca ttcatcttta ttaatttgta ctttactact
     4021 ctctataaat tcaaatacgc cttttggtcc acttttgaca cacaacttgc acatcttaca
     4081 gccagcactt atttctacta tacctgactt ttcttctata gccccaaaag gacatatttc
     4141 taaaattctc tttaagtcag ttattttatc ttgatttaca actatctttg acatttatta
     4201 ctccccctta caccaacttt aattcctcta atttatttac aagtgcaaaa cttagttcat
     4261 ctgcatttcc tcttacaata tttttatctg tatttggctt tggaggaaat attcttacaa
     4321 cttgagtagg agaaccgtta agtccatagt tattttcatc tttatcttca aagtcatcta
     4381 agctatatac aggtatttct ctatctttag tattaagttt taatttaaat gaaggtagtc
     4441 ttggttgaaa aatgtctttt tctacagtta ttaaacatgg atattcaatc tcacatactt
     4501 gtaaatcatt tggcatatct atttcaacaa ctatactttc atctttaact tcaattaatt
     4561 ttgtaacgtt agtaacatga ggtatatcta aaaattcagc tacttcaggt cccacctgtg
     4621 ctgtatcacc atcagtagtt tgttttccac aaattattag gtctaccttt cccatcttct
     4681 taataccttg tgatatggta taggaagtag ccaatacatc tgctcctcca aatctcctat
     4741 cacttattaa tgctccttca tcagctccca tcataaaact ttcttcaatt accttctttg
     4801 cctgaggagg acccatgcta agtactttta gattcgcttt cttatcttcc tttattctaa
     4861 gagctgtttc taaagcatat aggtcatagg gattcatttt tgaatctact ccatctcttt
     4921 ttagtactcc tgtagtttca tcaacttcta ctgaagtcgt tccaggaact tgtttaatac
     4981 atgtcaaaat ttccataaag tacactcctt ttttatttaa ctataactga tactccatca
     5041 ggtataacta ctactttgct ctccttacca actatactat tcgccatttc taaagcttct
     5101 ttaaagttaa atgcatgttt catatgcatt ttttttatca tttcaggatt acacttatct
     5161 gttaccatta tcacagtata tttagataat attctcgcta gtatctgaaa ttcccattgg
     5221 tctggaatcg tatctactct atcgacactt ctaattttat ctaaaagttg ttttggagaa
     5281 tctgcatttg ctacattttc ataaaaagat tgacctccat gaccatcatt acatgctgaa
     5341 atcattatta ttactccacc gtctttacaa gtagcttcag ctgctgtcat tcctttaaca
     5401 gattgataaa tattttggtc tagtggatat ccaccatttg tagttacaac aatatcagat
     5461 tttatagaat ttacttttga taactctgtt acaaatctac atcctttagt atgtgctttt
     5521 tcgacatcac cagcaaagga agcaattatt tttttatctt tatctattac aacattcaat
     5581 atgaatgcta attttgcaac tttagctgca tataccatat cctcatgaat aggattatta
     5641 ttcaattttc cagttcttga attatcacta tctataaagt cactacagtg attgtacatt
     5701 atagtctttg cagatgcgat tcctggaagt acactttttc tgccacctga aaaacctgca
     5761 aagaaatgag gttctataaa accttctgct atcaaaagtt ctgcctcata tgctagtttg
     5821 tttatatata aatctccacc tgaaggcaat atccctactt tacacataga tttatcatca
     5881 gtagatatat gcataactat atcttcatta tctactattt cttctcccat tttataaatt
     5941 agctcttccc ttgtacttgg cctatgaaat ccagttgcca ctataatctt cacatcaatt
     6001 tctggattaa cattcctaat ccttcttaat ataattggca tagttattct acttggtaca
     6061 ggtcttgtat ggtcacttgt aattatgact acattctttt tccccttaac cagttcttcc
     6121 agagaattag agcctatagg attattcata gattcttcaa ctatctcttc ttgagtctta
     6181 tgttctatat aattatccga ttttgactcc aaaacaccga gaaaacgttc atcaggtatt
     6241 tctaaaatca ttccttgttt tgaatacggt acttttaact ttaccatttt taatctccca
     6301 ttataataaa ctataaaatt ggtatgtggt cataccagtt ttacatttat atgatagtac
     6361 cattatctac catttgcaag aataatttat tttataacaa acttttaatt ttcatttttt
     6421 ctataatttt tacttataaa atccatatgt tcctttgctt tctcacatgc aagttctaca
     6481 tcattttcta caactgcatt gtatatagct tcatgctgtt taagcaatat attttcatta
     6541 aaaggttctg cgattatttt ttctcttgca tcttctataa atccatcaaa taattttgat
     6601 gctgtcataa aaagactttg aattaaataa tttttactta atgttgctag tttactgtga
     6661 aatttttggt ctaataacac cagtactcga ttattatctt ttttattagt ttctaccctc
     6721 atttcatcta ttatatgttt caattctata acttcagaag aagttattct ctttgaagca
     6781 agttttgcaa tctcaatttc taggattatt ctaagctcta atatatcttc aagtgtacca
     6841 ttattaagtt taaaaatcat agataaaggt tctattaaag ttttttctat atttgtacat
     6901 ataaaatttc cttcaccctg tctgctttct atcactccca tagtctctaa taccctaata
     6961 gcttccctta tggaagttct actcacattc atttgttcag acagttctct ttctgatagt
     7021 aatttatccc ccttttttaa ctctccattt agtattttat attgtatttg ctcaattact
     7081 tgttcataaa cttttttact agctatacta ttaaacattt tttcctccaa tatgataatc
     7141 gttgttgtaa tatatatcta ttatatatct taagccatta cattcatata tttttattac
     7201 ttttaatata atatcttata actcaaattt acaggtgata ttatgaaaaa aaataaaatt
     7261 attgcttttt ttgctccatc tatcatagtt ggagttctag ttttagggat tatcctatac
     7321 ccaaatgaag ctataaaatc tgcaagagaa ggcttttcta tatggtctaa tgttttagtt
     7381 ccatcactac taccatttat aattggagca aacttaattg ttgatttaaa aatagtggat
     7441 attatagggt ttatcatcaa tcccataact agatttgtat ttaatgtttc aggaaagagc
     7501 gctttagctt ttgctatatc tacagtatct ggttatccag taggagtaag tttagcttct
     7561 gaatttagga gtaatggaca aatttctaag tatgaagcac aaagacttgt gtctttttgt
     7621 tctacatcag gacctttatt cataataggt tcggttggta caggtatgtt ccaaaattca
     7681 aacttaggtt atttaatgat tctctgccat tatctaggaa ctatattggt tggtcttttc
     7741 tttagaaatt atggtaatga aaatcttcca aagacaaaat tggatttaaa aacaaatata
     7801 aaaaatgtaa tcaacattag aaattcaagt gggaatggtt tttttgtttt atttggaaat
     7861 gctgtattta atggagtaaa tacattactt acagttggtg gatttgtaat tgttttttca
     7921 gtagtattta aaatactatc tctttttaat gtaatctcat taatagcatc agttatatat
     7981 ttgcctcttt catttttagg tgttagtaaa gaactctgtc aagcatttgt aagtggtcta
     8041 tttgaaatta ctataggatg caataaagta tctagtataa caagttctcc agaaatttta
     8101 agagcatctt tagctagttt tttgatagga tttagtggat tatccatcct agctcaatgt
     8161 tgtacatttc tatcaaaaac ggacatagat ttaaaattat acatactaag taaatttgcg
     8221 catggagttt tagctgctat ctttatattt atactttatc caattgcaaa ctctgctgtg
     8281 ttggtttcta gctttgctga tacatacaac gttatatata ataatttaat ctggttttat
     8341 taccttagct attatgatac aattcttcaa attgtagtaa taatttatct aatctcagcc
     8401 gtatttattg caaaaaaaca tatgaaaact ttatctacaa atgggaaact aatgcgatat
     8461 aaaaatactt ttttcactaa gaaattttca cgaaaaaaat aaaactaaaa ataaaactaa
     8521 taaaacttaa ctaaatactt ccattttttt attatatttg tcataatttt aataaccttt
     8581 ttaattagtt taacagcata gttatttcat cacaatttaa taattttatt agaaatattt
     8641 ttcaaaaaa
//
LOCUS       DAEMHW010000160         8608 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEMHW010000160 DAEMHW010000000
VERSION     DAEMHW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.160"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            28..1596
                     /locus_tag="KPK67_002962"
                     /pseudo
     CDS             28..1596
                     /locus_tag="KPK67_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            1763..2680
                     /gene="pfkB"
                     /locus_tag="KPK67_002963"
     CDS             1763..2680
                     /gene="pfkB"
                     /locus_tag="KPK67_002963"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG2459897.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARSLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            2717..4636
                     /locus_tag="KPK67_002964"
     CDS             2717..4636
                     /locus_tag="KPK67_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2459898.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIFLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            5176..6138
                     /locus_tag="KPK67_002965"
     CDS             5176..6138
                     /locus_tag="KPK67_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2459899.1"
                     /translation="MNLQSKWVNFLKPTYVSDEIKRKKLQVIIFFSITYGLSYSLGLL
                     AICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFYSSVILFFIIYFFIFI
                     IESLKLISINQFQLMYLMLILISSLCVIVYSYKITSLYIFKNFKIGILLFFYFLFSQI
                     IFGLIISDNQFDYKNFLNYILLPIISLVYIYPFLCEEYGWRSFLQSILFDRFGKKIGI
                     VMVGTCWSLWHLPLQFTLYSPQTPIIGAIAHLIYGIGLSIFLGYVYMKTKNIWFCSIM
                     HVLINSLGLIFNDSEIIINYHSIIQRLIFILLFYTPFLFTKEYK"
     gene            6518..8044
                     /locus_tag="KPK67_002966"
     CDS             6518..8044
                     /locus_tag="KPK67_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG2459900.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKVAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            8072..>8608
                     /locus_tag="KPK67_002967"
     CDS             8072..>8608
                     /locus_tag="KPK67_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459901.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVT
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFI"
BASE COUNT         3410 a          791 c         1491 g         2916 t
ORIGIN      
        1 ctgtgacttt agtcatggga ggttcaggag gtaaatctaa atagcaatct ctatgatgat
       61 gtagatagag tagaatatat aatcagaaaa atattaacct taagtaatga agaaaaaaat
      121 actataaaac ttgaagattt agccgattct atgtatgtaa gtgtttcaac agtaaaaaat
      181 gatttaaaag aagtaaaaag aatattggaa aagtattcaa tatcagttaa atctaagcat
      241 aaacaaggta tatgtgtttt ggaaagtgaa gataagatat taaattgtat agttgactta
      301 agcaataaga gagataatca attgagttta aatgattttt taaatgatga tataaaggaa
      361 agaaaactat taataaaaaa attattatta gacatattaa atgaaaatgg attagtatta
      421 actgatatag aatttaagag tgtgttaaat aatatattag ttatgttaag cagacataag
      481 tataatgaaa aagaatttat agagaattat atagaaaatt ataggagcaa aagaagctca
      541 attttagaaa atgaacaaaa caaagaaatt ataataaatt caataaaaac attttgtaaa
      601 aatttaaaat tagctacatc aatagatata agtaatgata aggtatttga agagttttta
      661 tataagcata ttagtagttt atgtaaaaaa ataaagttag gtataaatca atcacctata
      721 atggctcaag atataaaaat aaaatatcca tttgcttttg aactagctaa tatagctaaa
      781 aagacaatag aaaaagattt gaagatagaa attaatgaag atgaagttgc aaacatagct
      841 ctacatatag gaggagcagt tgaaagagca tcttataata ataaagataa ggttttcaaa
      901 actataatag tttgtacttc aggcataggc acatcaatgt taataaaagc taaattagag
      961 aatatattta aggaaaaatt agaaatacta aagattattc cttcatactt agttgaatat
     1021 ataaaagtta tagatgttga ttttgtaata tctactgttc ctattgaagt aggagatgtg
     1081 ccagtaataa atatatctcc aattttaagt gagaaagaaa ttagattaat tgaaaaatat
     1141 atagaaacag gaaatatata cctagattta gatataaaaa gcctgtttga tagtgaatta
     1201 ttttttactg atttggtgtt taaaaggaaa gaagaagtta tagattttat ggcaagtaaa
     1261 cttgtacaaa agggctatat agatgaggat atgaaaaaat cttactttga gagagaaaaa
     1321 atagcaacaa cagaaatagg gaatatggta actataccac atggagctat tggaaaaatt
     1381 tttgataata aaatagcaat tggtatatta aagaagccta tttcttggga aatatcagat
     1441 gttcgtttaa taataatgtt agcccttgat aaagataaaa tattagatta tgaagtaata
     1501 ttttcaaaaa tatataaaag agtagattct atagcaaaag ttataagtat atgtgaaaat
     1561 agtagttttg aaaaatttgt aaatttattt aattagatgg gtagtattgt atatacacct
     1621 gtaaatttgg ataatataaa actaaataag ataaaaacga taaatttctc ttttaattaa
     1681 gagaaatttt ttgactttca aggatattaa tataaagtta agatataaac ataaagcaaa
     1741 taaataaaaa agaggtggca atatgattta tacagtgaca ttaaatccat caatagatta
     1801 tatagttaag ctagatgagc taaagacagg aagtacaaat cgtgtaaaag aagaatacgt
     1861 ttatccagga ggaaaaggta taaatgtatc tcgcatactt aaggaacttg gaaatgataa
     1921 tacatcttta ggttttataa gtggttttac tggagaatat ataataagaa cattggaaga
     1981 aaaagaattg aagacagatt ttataaagat aaaaagtggt ttttcaagaa taaatgtaaa
     2041 aataaaggcg ttaaaggaaa ctgagattaa tggacaaggt ccaaatatag atgatgaaga
     2101 cattgatact ttatataaaa aacttgataa gctaaatcaa gatgatattt taatattggc
     2161 aggaagtata ccatctactt tagatgaaaa actatacgaa aatataatgg ctagattaga
     2221 aaaaaagaat ataaaagttg tagtagatgc aactaaaaat cttcttctta atgttttaaa
     2281 atataaacct tttctaatta agcctaataa cgacgaatta gaagaactat ttggtgttaa
     2341 attaaattca attgaagaca tggtaaaata tgctagaagc ttaaaggaaa tgggagctat
     2401 aaatgtcttg gtatcaatgg gaaaagatgg agcattgttg attacagagg aagatgaagt
     2461 gcttataagt gatgttccaa aaggaaaagt aaaaaattct gtaggagcag gtgattcaat
     2521 ggtagcagga tttatatctg ggtatttgaa tactgggaaa tatgattatg ctcttaaatt
     2581 gggagcagct tcaggtagtg caactgcttt ttcatatgac ttagccaaaa gagaatacat
     2641 tgataaattg gtaaatgaga tatctgtaaa gcagttttaa aatattaaaa aatatataga
     2701 aaggtaatta atatttatga aaattataga tttattagat gaaaagtcaa ttaagttaaa
     2761 tttaaattca aaaaccaaat cagaagcaat agaagaatta gtagatttgg tcgcaaatag
     2821 tggaaattta aatgataaag aaaactacaa aaaagcaatt ttggctcgtg aagaaatgag
     2881 tacaacagga attggagaag gtgtagctat acctcatgct aaaaattctt cagttactaa
     2941 agcttgtata gcagcagcag tgtcaaaaga aggtatagat tatgaatcgt ttgacggaag
     3001 tttatcaaat ctatttttta tgatagctgc accagacgga gctaacaata ctcatcttga
     3061 agtattatca agattatcta caatacttat ggatgaagac tttagaaata aacttataaa
     3121 ttcaagctct gaaaaagaat ttaaggaaat aatagataaa aaggaaagag aaaaattcag
     3181 tgaagaatat caagatgaga aagttgctga aaaaaatata atagatacta aagagaatga
     3241 tgctaataag tatcctaagg ttttagcagt aacagcatgt ccaactggaa tagcacatac
     3301 atttatggca gctgaaagtt taaataaaat ggcagaaaac aaaggtgtat caataaaagt
     3361 agaaactaat ggttcagcag gtgttaaaaa taagcttaca aaagatgaaa tagaaaatgc
     3421 aacatgtata atagtggcag cagataaaaa tgttgagatg gctagattta atggcaaaaa
     3481 agtcataaaa actaaggtgg ctgatggtat acacaaagcg gaagaactta tagacaaggc
     3541 tgtaaatgga gatgctccaa tttaccatgg aggagatgga agtcacaacg aatcaaatga
     3601 agaaagtgaa agtggattta gaaaagtata caaacactta atgaatggtg tatctaatat
     3661 gttaccattt gttattggtg gagggatact tatagcgata gcctttttac ttgatgatta
     3721 tactattaac ccttcaaact ttggttcaaa tacacctata gcagcatttt ttaaaggaat
     3781 aggagataaa gcttttggat ttatgttacc tgtacttgct ggatatatag catactctat
     3841 atctgataga ccagcgtttg ttgtaggatt tgtaggaggt gctctagcag gtgatggtgg
     3901 ttcaggcttt ttaggtgcac tacttgcagg ttttatagca ggttatttag tagaagggct
     3961 taagaaaata tttagtgtac taccagcttc attggaagga ataaaaccag tattgttata
     4021 tcctttactt ggaacacttt tgatgggaat tataatgaca tttttagtaa tacctccagt
     4081 tacagcaatt aataatgcaa tggttggatt tttaaatggt ttaggtggaa cttcaaaaat
     4141 atttttagga ttagtattag gtggaatgat ggctgtggat atgggtggac ctgtaaataa
     4201 agcagcatat gtatttggag ttgcatcact tgaatcaggg cagtttgaaa taatggcagc
     4261 agtaatggct ggtggtatgg ttccgccact tgctatagct cttgcaacta cattttttaa
     4321 gaatagattt actaaagaag aaagagattc tggtaaagtt aattatgtaa tgggattatc
     4381 atttgttact gaaggagcga taccttttgc agctggagac ccacttcatg ttataccagc
     4441 ttgtgttgga ggttcagcag tggcaggagc acttagtatg ttgttcaacg cagctcttcg
     4501 tgcacctcat ggaggagtat ttgttatacc agtggttacc catccatttg catatatatt
     4561 agctatagct gtaggtgctt tggttggaat gatgctttta gcattactta aaaaaccatt
     4621 aaatcaagaa gtatagcaat taaaggatat tcaagaactt aaaatatgat gttttataaa
     4681 aattaatata ttgctgaatt attttaaaat aaggatttta tgaggctgtt atataacgat
     4741 ttgtaattgt tatcttgaca gtcttttatt tttatataag taaaataagt gtgttttgct
     4801 ttatttgtat ttaaattgtt tttaagttag aatataaaga aaattatata ttattttaat
     4861 attcactcga actagaaaat aatatttgtt gtattttttg tatataaaaa tatattgtaa
     4921 tattaaaaaa ataatagtaa actaattgca taaaaatgat ttttatataa attttaaaca
     4981 gaagaataaa tttctaacga aattggatat ttcttctgta aatacatatt caaaaaaaga
     5041 attataagag tttagtcagc agtcgtttaa gtaataaaga atttataaat atatagaaat
     5101 ataatgatat gagtataagt agaaaaattt tatgattttt acaattattt tttataaaaa
     5161 gggaggttat gtttgttgaa tttacaatca aaatgggtaa actttttaaa gccaacatat
     5221 gtcagtgatg aaataaaaag aaaaaaacta caagttataa tatttttttc tataacttat
     5281 ggtttatctt attcactagg tttattagca atatgtttta atcatttaat agattcagaa
     5341 attttagctc aatttatgat gatattgcca ttatcttcag tttctattgc aaaattttac
     5401 acagagggaa gaaataatga taaatataat ttttattcat cagtaatatt attttttatt
     5461 atttactttt ttatatttat tattgaatca ttaaagttaa taagtattaa tcaatttcaa
     5521 ttaatgtatc ttatgttaat tttaataagt agtttatgtg ttattgttta ttcttataaa
     5581 ataacaagtt tatatatttt taaaaatttt aagataggta ttttgctatt tttttacttt
     5641 ttattttctc aaattatttt tggattaata ataagtgata atcaatttga ttataagaat
     5701 ttcttgaatt atattcttct tcctataata tcattagtat acatttatcc ttttttatgt
     5761 gaagagtatg gttggagatc ttttttgcaa agtattttat ttgatagatt tggcaaaaaa
     5821 ataggtattg taatggtagg cacttgttgg agtttatggc acttaccttt acaatttaca
     5881 ctatatagtc ctcaaactcc aataattgga gctatagccc atttgatata tggtattgga
     5941 ctttctatat ttttaggtta tgtttatatg aaaactaaga acatatggtt ttgctctata
     6001 atgcatgttc taattaatag cttaggatta attttcaatg attctgaaat aattattaat
     6061 taccatagta ttatacaaag attgatattt atactattat tttatactcc ttttctattt
     6121 acaaaagaat ataagtaaga attacaatta atttatataa aataaatttt taagtaaata
     6181 tttttataga gagattgtta tattacatat cagaaaaata ttaaactaac taaatcaata
     6241 aaaaagttgg tggctaacag tttataattg ttagcacaac atcatcttat gtattgaaat
     6301 ctaaaatatc ctcattttta cttagcataa atactattaa ttaagaaaat aatatagaaa
     6361 ttataaatat atataagttt agtctggtgt ataaaacaag aaaactaagt gatttttaat
     6421 ttagcttttt aataagctta ttttttatac tatttataat aaataaactc catgatggga
     6481 aaactattac tatttaatat gtaaaggagt attttaaatg aatgaatcat attggttttt
     6541 aaactcaagt ccaaaagaat ataacaaatt aggtgaaaat ataaaaacag attgtcttat
     6601 agtaggtggt ggaataactg gattgacaac tgcatattta ttagcaaaag aaggtaaaaa
     6661 agtagtgctt gttgaggctg ataaaatagg atatggaact tctggaagga atactggtaa
     6721 ggtaacttgt cagcatgata tattttactc taagatagag aaaaaatatg gattagataa
     6781 agcaaaatca tattataatg caaataatga agctttaaat ttagttgaac aaataataga
     6841 agaaaacaat ataaaatgtg attttaaaag ggaaacttct tttgtattta cagaaaaaga
     6901 ggatactata aagaatatta aagatgaata tcgtacatgt aaaaaaatag gaataaattg
     6961 tgagtatcat gaaactatag aaaatattcc attagatata aagggggcta ttagttttac
     7021 taatcaaggt caatttaacc caaagaagta tattgatgga ttggcaaaag tagcagtaaa
     7081 tttagggcta aaaatatatg aaaatacacc agtagtagac cttgaaaaag gaaaaatatg
     7141 tagggttaaa acaagagaag ataatataat agaagctgag aatgttataa tttcatctca
     7201 tagtccatgg tatgatggat tgaattttta ttttgcaaaa gaatatgcag agcgtgctta
     7261 tttaatggtt gcagttttgg aaaataaatt agcagatggt atgtttataa gtatagatga
     7321 cccatcaata acttttagac aatataatga tggaagtgaa aatttactga tttttggagg
     7381 aggagaccat aaagttggtc agggaggaac tgagaaagaa atatttgatg atttagagca
     7441 ttatggtaaa gaagtcttta aggtaaaaga ttttaagggg aaatggtctg ctcaagataa
     7501 tatgagtttt gacaatgttc cttatattgg ttatatcaac aaaagagaag acaatattta
     7561 tgtagctaca gggttctcta aatggggaat tactaatgga actgctgctg gaataataat
     7621 aaaagattta ataataaata ataattctga ttataaagat acttttaatc catccagact
     7681 aggtagctac ttttcaaaag attttataaa ggaaaatgca aacgtagcaa taaattatgt
     7741 aagtggaaaa ttaaaaatag gtagtggaga tatgcctaaa aataatggag aaggaaagat
     7801 tgttaatatt gatggaaaaa gatatggtgt atataaggat gataatggag atttttatat
     7861 tgtagacacg acatgtactc atttaggatg tgaacttaat tttaacagtg aagaaaaaac
     7921 atgggactgt ccatgtcatg gttctagatt tgactataag ggaaatatac ttgaaggacc
     7981 agctcttaag ccattaaaac tttatggaca tggtgataat gatgttaatc ctaagttatt
     8041 ataaaattat ttttaggaag gtaaagtgta tatgaataaa cttttaaagc caataactat
     8101 actattgtat atagtttata ttttatctgc cgtatttttt gtagcaaaca aagaatatgg
     8161 aagtctaggt ctagtagcaa ttagtttagt tggtactttc attttgagtt ttataaataa
     8221 gcaaactgaa agattattga gtaaagaatt atatataatg cttgctttat ttataatgtt
     8281 ttcatcattg ttaggtactt gttttaattt ttatgatata aattattatg atgatttttt
     8341 gcatttatgg tcaggtataa tatcttgtat tgtgactttt tctataatta aatactttta
     8401 tacacaaaga gagataagta aaatgagtaa agtgttttta gttatatttt tatttatgtt
     8461 tagtatggga gtagcgagtt tatgggaaat aggtgaattt ttaatggata cttttgttgg
     8521 gactactaca caggccggag ggttagaaga tactatgatt gatatggtag atgctttaat
     8581 aggtacaatt ataactattc cttttata
//
LOCUS       DAEMHW010000161         8434 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEMHW010000161 DAEMHW010000000
VERSION     DAEMHW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.161"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(380..1651)
                     /gene="sleC"
                     /locus_tag="KPK67_002968"
     CDS             complement(380..1651)
                     /gene="sleC"
                     /locus_tag="KPK67_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBG2459902.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELVFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            1896..2612
                     /locus_tag="KPK67_002969"
     CDS             1896..2612
                     /locus_tag="KPK67_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBG2459903.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            2691..4001
                     /locus_tag="KPK67_002970"
     CDS             2691..4001
                     /locus_tag="KPK67_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBG2459904.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFIGEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            4101..4637
                     /gene="lepB"
                     /locus_tag="KPK67_002971"
     CDS             4101..4637
                     /gene="lepB"
                     /locus_tag="KPK67_002971"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG2459905.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            4803..5339
                     /gene="lepB"
                     /locus_tag="KPK67_002972"
     CDS             4803..5339
                     /gene="lepB"
                     /locus_tag="KPK67_002972"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG2459906.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            5349..6104
                     /locus_tag="KPK67_002973"
     CDS             5349..6104
                     /locus_tag="KPK67_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBG2459907.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            6178..7845
                     /locus_tag="KPK67_002974"
     CDS             6178..7845
                     /locus_tag="KPK67_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBG2459908.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
BASE COUNT         3167 a          858 c         1572 g         2837 t
ORIGIN      
        1 aataagaagt aatactctaa gtgatagaca gtgttttaaa taataaggag taatactcta
       61 agtgagaagt agaattttaa ataataagtg agattttaaa taaaaagtag gtttttcgtt
      121 agaatagcat tttattaaac ttaaaaataa atataatatt ttaaagaaat ctaagttgct
      181 aagcactgtt tttaagaagc ttaaaatccc ccatttaaag ttttaagatt ctaaaaaaac
      241 aagttataac taaaaaatgg ctgttaagac tacaatactt ataagtaata cttatggtat
      301 atattagttc ataacagcca tttttatttt aaattaataa ttttattcaa taatttgagt
      361 ttatgttcta aatcaagctt taaattaaag gatttaaaga agctattcta gttgtagcta
      421 cataaactct ggatatttca taccatgttt taaaatctac tactccgctt tgaggtaaac
      481 caaagattcc ttggaatatc ttaacagcgt tttctgtatc tgttccatag ataccatctt
      541 ctataacttt tggtacagct ggataagaat tagatatagc atttaactga ttttgtatgg
      601 ttctaacata ctgcccagaa gaaccaactt gtagtgtagt accagggaat gacactggta
      661 caccacttac tataggtgct ctttcaaata ctatctcatc tccatagaaa tatcttagta
      721 tactctcata gtcatatcct tggtcaccta agtctttact gccccattga gtcatttggt
      781 caggacattg ggatttttgt ccatcacaat attgtgctaa tagtggctgt cttgatgtag
      841 gaggtctttt tataaaagta ttaaaaatag catctacaac tacgttaatt ggttcaaata
      901 agtttctatt atttataaac ttatggtcgt atgctgttgt tgaagttatt gtaaagttat
      961 atcctttatt tctataccat tcagtaaaga ctctatttaa agtaaatgat attatagcta
     1021 ttacatttgc ataaattgtc tgttcaggcc aagttgaata tatttcagat gacgctatgt
     1081 ttttaatgta ctctttaaat ggaatccaat agtttggagc tgatgaatct tctggcaaac
     1141 catcatgaac tactataaat tcaggcacta cagggttatc taatacaacg aaaccagtag
     1201 gaggtggtaa tggttttaag ttactttctg gaatcttagg aggataagta ccatatagag
     1261 tatgttctcc tatgtcaaaa accaactcac tttgtcttga gaaagaacgc ttacttcttg
     1321 ttctaggaga catgggaact ccttgtcttg cttcaattgt agctaacaac tgtgttcctt
     1381 ctatgacaac tgtctcatat ccatctgcta tggcttccac tatatactgg ctataaggtc
     1441 taacatcaga tggttgtaga gagtaatcta catctggtgc tggtaatact aaaccagtca
     1501 cttgaccaga ttcattactt cttaggtttt ggtataaagt tgatgagctt tggctaccat
     1561 tagacattga atatatgttt actactgcat tttgaatagg tctattgtta gtagcatcaa
     1621 ttatgcttac tgttaagaaa ccatcttgca tcaaatcacc ctttctttaa atgaatttag
     1681 ttcccttata tttcaagata tgaatatagc ttgaaatttg ttaaataata tagatataaa
     1741 tttaaatttt tttgagtatt tgagtatatt tgtatttaaa taatttaaaa taatttaatg
     1801 tatattttag tatgtattta aaacacgaat aagttaaaaa taagtagatg atataaatca
     1861 taaataaatc aaaaattttg aaaggaaaat aatctatgaa gctagattta tttttattag
     1921 caatagttcc tatattgata gggatgtttt ggataaggtc taaagataga tattgtagag
     1981 aacccctaat ccatctaatt aaattttttt taataggtgc ttttctaagt gttataatta
     2041 tacttttaga aaatttactg atgaagttta atgtatttga agggtattca gaactaatat
     2101 atgtatcatt tgtagttgca ggacttgtag aagaaggtgt aaaagctctg atactgattc
     2161 ctgccctaat taaagaaaaa cactttactg aaaagttaga tggtattatt tactctgtat
     2221 ttttagcttt aggatttgca acaattgaaa atatggttta tattttttct gaaagtagaa
     2281 atctagcact gcaagttggt attaataggg cagttatctc tataccagct catgtaatgt
     2341 ttgcaattac tatgggctac tatatttcta agtataaatt tgagggaaat aaaaataaaa
     2401 gacgtgagta tttgtttatg gcggtgttaa ttccaatttt attacatgga gtttttgatt
     2461 tcatattgat gatagaatat agatgggcta ttatattact tatagtttat gtaataattt
     2521 tgtggaagat aaatcttgat aaacttgaga aatatatgaa tcactctaaa aaagtgtttt
     2581 tcggtaatct taggaaaaag aagaagaaat aaaatttaaa ggaaagacat aaataattaa
     2641 agtataataa atgacacaca agtaaagcag attgaagggg gattatagat ttgacagaat
     2701 taccaaagga atttttagaa gatattgaag aaatactaaa agatgaatat gatgatttta
     2761 taaaaagtta tgaggaaagt aagactactg ggcttagaat aaatacttta aaaattgaca
     2821 agaaaaaatt atttgatttg gagttgtttg atttgaatca aataccatgg gcagaagaag
     2881 gtttttatta tgatgataaa attgacagac ctggtagaaa tccactgcat gaggctggag
     2941 catactattt gcaagagcct tctgcaatga gtgtaatacc aaaggcagat attaaagagg
     3001 gtgaaaaagt tctcgatatg tgtgctgctc ctggtggaaa atctacatat atactatcta
     3061 agctaaacga tacaggttta ttggtttcta atgaaattaa tgctagtaga attaaggctc
     3121 taggggaaaa tttagagaga tttggagcga gaaattgtat tataacaaac accgattcta
     3181 atagcttaag aaagacgttt aatgggtatt ttgataaggt tattatagat gcaccttgtt
     3241 ctgggcaagg tatgtttagg aaggatgagg ttgccatatc agattggagt tatgcaaagg
     3301 tagtagaatg tcaatccatc caaaaagaga ttataagaga tggatataaa atgttaaaga
     3361 aggatggcat tttaatttac tcaacttgta ctttttcaag ggaggaaaat gagcatgtta
     3421 taaatgagtt tatcggtgaa tatccacatg cagaacttat tgaaatggaa agattatggc
     3481 ctcataagat aaagggagaa ggtcattttg ttgccaaaat aaaaaaatta gaagaagaag
     3541 aatgtaatgt caaagaatta aaaataaaaa atttagataa agaaataaag gattataggg
     3601 agtttgaaaa gaaattttta aaggttgatt tttatagtaa taaaaaaaat aaattttatt
     3661 taagaggaga aaatttgtac ctacttcctg aaatatatcc agatacgaaa aaactaaaag
     3721 tattgagata tggtttgcat ttaggggttt taaagaagaa tagatttgag ccttctcatg
     3781 cactatctca ttacttaaaa gttgaagatg taaaaaatgt tgaaaacttc agtgtacaga
     3841 gtgaatcaat tcttaaatat ttaaaagggg atgttgtaaa ttctaatgat agtagaggat
     3901 gggttttggt ttcggttgaa ggaatacctt taggttgggg aaaagaatct tctggtgtaa
     3961 ttaagaatca ttatcctaaa ggtcttagaa aagtatttta aactattaga aaaaagaagt
     4021 ataatattta tattgtaggt ttatagtaga ttattttgct gtagattgac ttaatattta
     4081 acacagagga gacgatttta gtgggtgaag cagttaaaaa agaagttgta gaatggataa
     4141 aagtgattgt catagctctt gttttggcat ttgcaataac tcgttttata gtgccaacaa
     4201 tagtcaaagg agaatcaatg tatcctacat tagttgaacg tgattatttg atagttaaca
     4261 gaattgcgta caaggtagga gagccaaaat acaaagatat aatagtattc aaaaccgact
     4321 taacagagga aaatggaaag aaaaaagatt tagtaaaaag agttatcggg gttcctggtg
     4381 accatgtaaa aatacaagac tccaaggtat atgtaaatga taagttgtta gatgagactt
     4441 cctatataca taataatcgt actgatggag atattgatat cgtagttcca gaaggaaaat
     4501 tatttgcaat gggagataat agagaaaaaa gtttagatag tagatacgat gaggttggat
     4561 tggtcgacga gcataccatt ttaggaaagg ttctagtcag attgtatcca ttttctaaga
     4621 taggaactat tgactaaaca tataaaattt ggagcaatcg ctgattagct gaaatcacca
     4681 gaccatacac aatagttgtc tatataaaaa agacaacacc ttatttagta gacttatcta
     4741 aaagatagtg tttaagagac attatcaata aagataatat aactataaag gagaatttgt
     4801 gaatgaatga aactattaaa gaagagattg tagagtggat aaaaataatt attactgcac
     4861 ttttttttgc atttattata actcgtttta taaaaccaac attagtaaat ggagaatcaa
     4921 tgtacccaac acttaaatca catgattatt tggtagcaaa caggatgaca tataagttat
     4981 cagaaccaaa atgtggagat ataatgatat ttaagactga tttattacaa gagaatggaa
     5041 ggaaaaaaga gcttgtaaaa agggttatag gtgttcctgg tgaccatcta aaaattaagg
     5101 attctaaggt ttatataaat ggtaagttat taaatgaagt ttcatatata catgataatt
     5161 atactgaagg cgatattgat atggtgattc ctaagggaaa agtatttgcg atgggagaca
     5221 atagagaagt tagtttagac agtagatata aagaagtggg attagtagat gaagaaaata
     5281 ttaaaggaaa agttatttta agagtatttc cttttacaga tataggtatt tttgagtagg
     5341 tgatttgtat ggcagttggt atagcgtcct tattgtttaa tattgaggag gctttaaaca
     5401 tttgtgagag tataaagcaa ataactcatt tagagattgg aattgacaat atatctgaat
     5461 gtagtgagtt atgtaaatat aaagagagaa tttctaagtt aggattatct atagggatac
     5521 atttgcctat ggaattaaat acttgcgaaa acatagagta tatacgaaat tcatggataa
     5581 attttattga gaagatagaa tttgaactaa aaggatttga tttaaggtat tttaaccttc
     5641 atttgggata tgttatgaca aatagagtta gaaagaatag agacaaatat ttaggaaata
     5701 gtgtggattt tttagataaa ttaaatacaa attcatatgt atgtatagag aatacttatt
     5761 caaaaggtgg ggatttttca aatattggaa atatatctta tgattttgaa tatatattta
     5821 agaggattaa aaattctaag atttgttttt gttacgatac tggacactgt ttaattgatg
     5881 aggatgctta tgtaaagaac ttaaaggata aaataagact gatacactta agtgataatg
     5941 atggtatcaa tgatacacat gttggtatag gaagaggaat tttatctgaa gaggggatta
     6001 aagaggtctt aacattagat gcagaatatt tagtattaga aataaattac gaagacattg
     6061 aagatactat aagtaaattg aataatattg taggagaggg ttaaatacct ctctttatct
     6121 tgttgatatg cctcattaat taaaatttat attcatcaaa aggaaggaga tgttgaagtg
     6181 aaaaaaataa acttaaaaat agaaggaaaa gataaggaat attcattaga agaaaattcc
     6241 cctggaatca gattgggaga tatagcaaaa gaattttgtg atgagcataa gggttatata
     6301 acacttgcag ttgtagacaa taaactaaaa gagctaaatt gtagagttaa gaaggattgt
     6361 gagataaatt ttttagatac tactaatgaa gatggagaga gagtttactt tagagtcatg
     6421 tcatttatat ttgtaatggc atgtagagaa atcttttggg atagcagagt tacaatagaa
     6481 cattcactat cagatgggct atattgtgaa gttcatatag atagaaagct taaagaagca
     6541 gatgttgaaa atataaaaaa caagatgaaa gagatagtaa acaacgatta tgttatagaa
     6601 aaaattgaag ttacaagaaa tgaagctatt tctatctttg aaaataatga aatgtatgaa
     6661 aaggcagagc ttttgaaata caaagagtat gatagtgcta aagtatataa gtgtagaaat
     6721 tatatagacc atttttatgg ttatatgttg ccatcaactg gttatataaa atcatttgat
     6781 attaagcttt ataatggagg cgttataatt ttaggaccta gtgaagaaga taagactctt
     6841 cctatgaaat ttgtacctca acctaagcta tcaagtgtat attatgaagc agaagaatgg
     6901 tcaagactta tgggaattga taaggtagta tctcttaata aaataataga aaacaatgaa
     6961 tatggagata taataaggac atttgaagca ttgcatgaaa agaagttatc ccaaatagcg
     7021 gatatgataa aggatggcaa taagagagta gttttaatag cagctccatc ttcatctgga
     7081 aaaacaagtt ttgctcacag attatcaata catcttagag taaataattt aaatcctatt
     7141 tcaatttctt tagatgatta ttttatagat agaaaacata ctcctttaga tgaatatgga
     7201 aattatgatt ttgaatcaat ttatgcaata gatttagaga agtttaactt agacttaaag
     7261 aaacttttag ctggtgaaga aattgatgat ataagattca attttaaaga aggtaagaga
     7321 gaatacactg gtaaaaagat aaagatagat tcaaatcagc caattatatt agaaggtata
     7381 catgggctaa atcctatttt aacatcatct ataccagatg aagataaatt taagatatac
     7441 ataagtgccc ttactcaaat aaatttggat gaccataatc gaatccctac tgctgatttg
     7501 agaatgataa gaagaattgt tagagactat aattttagag gatatagtgc tgataatact
     7561 attttacaat gggcatcagt tagaagagga gaaaagaaga atatattccc gtaccaagag
     7621 gaggcagatg ctatatttaa ctctgcttgt gtatatgaat tggcagtttt aaaacgattt
     7681 gcgaaacctt tgttggaaga gataaaggaa gataatcctg cacatattga agctactaga
     7741 ttactaaagt ttttgcagta ttttgttgaa ttggacgata cttctgacat accaggtatt
     7801 tcaattctta gggaatttat tggaggaagt aaaatagttg attaatgtgt gtattataga
     7861 ttgttttatg aagattaaat ggcatagcaa gtagtgtgat tggtttatgt taagtatatt
     7921 aagtgtaaag tggagaatta aattgataca gattttaaag tttgagttga tatataaaaa
     7981 tgcttgtttt atacttttgt atgaagcaag ctttttattt taaattttac ttttttgttt
     8041 aatattttat tagtttatgt gattattttt taaaagagta aaagagtaaa agagtaaaag
     8101 agtaaaagag taaaagagta aaagagtaaa agagtaaaag agtaaaagag taaaagagta
     8161 aaagagtaaa aggtgttggg ttagaaattt ttggtgttat aaaaatttaa attactggat
     8221 tattagattg ctggattgct aaattactag gttgttaaat tattgaatta ttaggttgtt
     8281 gaattactaa attactaggt tgttaagtta ctggattatt tagatagtta gattactagg
     8341 ttgttaaatt attgaattat taggttgttg aattactaaa ttactaggtt gttaagttac
     8401 tggattattt agatagttag attactaggt tgtt
//
LOCUS       DAEMHW010000162         8418 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEMHW010000162 DAEMHW010000000
VERSION     DAEMHW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.162"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1334
                     /locus_tag="KPK67_002975"
     CDS             <1..1334
                     /locus_tag="KPK67_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2459909.1"
                     /translation="VLLIAISYLMSISYFIAENIMLSVFILVTIIPGTFLFFSSTMYM
                     IIDAVKKRKSFYYKGQNLIAFSELGFKLKSNSSVLAVIAILIATSVTMLGFTISLYYD
                     IDRNISENYKYSYTINLGNSYVNNEIDKLLYKYKKDNKIIFDKTIELIDKDINIEMYY
                     KNGNSYIEDRTSVDIIRESDFKKLRQYQNNNYEELTSKESVYYVSDSYKKIFFKDFEI
                     EKINLHIKDTNEYDINLFKVQKSILEPEINSQATFDLIVVKDDVFNQSKLHGNSRLIR
                     LIDIKNEKKELDLTLKLKNIVSENMKFTYPFNFTSSIESYNNLIKLSGLMLFIGIFLS
                     VVFLLCTGSIILFKQLSNIYDDKERYIMLIKLGANNKDIEKIISKQLKVIFLMPLVVG
                     TVHNLFAMSIAQKFIPRSLLVPIIITLVIYFIGYFIYYFLTLKYALNMIKE"
     gene            1559..3172
                     /gene="ggt"
                     /locus_tag="KPK67_002976"
     CDS             1559..3172
                     /gene="ggt"
                     /locus_tag="KPK67_002976"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBG2459910.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKYGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(3305..4156)
                     /locus_tag="KPK67_002977"
     CDS             complement(3305..4156)
                     /locus_tag="KPK67_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBG2459911.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            4782..5675
                     /locus_tag="KPK67_002978"
     CDS             4782..5675
                     /locus_tag="KPK67_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBG2459912.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            5675..7069
                     /gene="gltA"
                     /locus_tag="KPK67_002979"
     CDS             5675..7069
                     /gene="gltA"
                     /locus_tag="KPK67_002979"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBG2459913.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            7435..>8418
                     /locus_tag="KPK67_002980"
     CDS             7435..>8418
                     /locus_tag="KPK67_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBG2459914.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRK"
BASE COUNT         3315 a          917 c         1437 g         2749 t
ORIGIN      
        1 gtgttttatt aatagctatt tcttatttaa tgtcaattag ttattttatt gctgaaaata
       61 ttatgctttc agtttttata ttagttacaa taatacctgg aactttttta tttttttcat
      121 caacgatgta tatgattatt gatgctgtta agaagagaaa aagtttttat tataagggac
      181 agaatttaat tgcattttct gaacttggat ttaaattaaa gagcaatagt agtgtattgg
      241 ctgtaatagc aatattaata gctacaagtg taactatgct agggtttacc atatcacttt
      301 attacgatat agataggaac ataagtgaaa attataaata cagttatacc ataaatttag
      361 gaaattctta tgtaaataac gaaattgaca aattattata taaatataaa aaagataata
      421 agataatctt tgataaaaca atagaactta tagacaaaga tataaatatt gaaatgtatt
      481 ataaaaatgg aaatagttat atagaagata gaacgtctgt agatattatt agagaaagtg
      541 attttaaaaa attaagacaa tatcagaata acaattatga agaattaact tcaaaggaat
      601 ctgtgtacta tgtttctgat tcatacaaaa aaatattttt taaagacttt gagattgaaa
      661 aaataaactt gcatattaaa gatacaaatg agtatgatat aaatctgttt aaagtacaaa
      721 aaagcatctt ggagccagaa ataaattcac aagctacgtt tgatttaatt gtagtaaaag
      781 atgacgtatt taatcagtca aaattacatg gaaattctag attaataaga ttaattgata
      841 taaaaaatga aaaaaaagaa ctggatttga ctttaaaatt aaaaaatata gtaagtgaaa
      901 atatgaaatt tacttatcct tttaatttta catcaagtat tgaaagttat aataacttga
      961 taaaattaag tggtctaatg ctgtttatag gcatattttt gtctgtagta tttcttttat
     1021 gtacaggtag tataatctta tttaagcaat tatcaaatat atatgatgat aaggaaagat
     1081 atattatgtt aataaaactt ggagcaaata ataaagatat tgagaaaata atatcaaaac
     1141 agttaaaagt tatattttta atgccattag ttgtaggtac agttcataat ttatttgcta
     1201 tgtctatagc acaaaagttt ataccaaggt cattacttgt accaattatc ataactctag
     1261 tcatttattt tataggatat tttatatatt attttttaac tttaaaatat gctctaaaca
     1321 tgataaaaga ataaattatt tttaagtcaa aagaattaga tatatgtttt gaataaaata
     1381 acttatttaa gtgcagatag aaaatactag aattgagttt aagatattta tttaaagata
     1441 aaattaaagt taggaaaaag tctatatttt atatcatttt gtatcagtta cattacttga
     1501 tgttatataa taaatctaag cagaatatca aataaaaaat gacgaggagc tgaaatttat
     1561 gaagtttaat gcctaccaac ataaatactc atcaataaga aatgtcgtgt atgctaaaaa
     1621 tggagcagtt gcaacatcaa caccattagc atctcaagct ggacttgaaa tactaaaaaa
     1681 gggtggaaat gctgttgatg cagcagttgc aacagccgcc acattagcag tagtagaacc
     1741 tacaagcaat gggattggag gagatgcata tgcccttgtt tggatagagg aagaaaaacg
     1801 attgtatggt ttaaattcta gtggctttgc tccagaaaat atggaattaa aaaattataa
     1861 taatatgaaa gaaatgccaa aatatgggtt tggagcagta actgtacctg gaataccagc
     1921 agcttggtca gaattaaata aaaaatatgg taaacttagc ttaatggaat gcttatctcc
     1981 agcgataaac tatgcaagag agggatacgt agtatctcct aatgtagcta aggtatggaa
     2041 aaaatcatat gaactctatg aaaaagaact tattggagaa gagttcaaac catggtttga
     2101 tacattttct aaagatggta aagctccaga ggctggagat atatttatat gtgaagaaca
     2161 ggcaagtaca ttagaagaga tagcaaatac tcaggctgaa agcttttata gaggaagact
     2221 tgctgataaa atagatgaat attctaaaaa atttaatggg gctataagaa agagtgactt
     2281 agagtgtttt tatccaacat gggtagaacc tataagtaca gaatataagg gatataaaat
     2341 atttgaaatc ccaccaaatg gtcatggaat aacagtttta atggcactta acatattaaa
     2401 agaattggaa ttagaaggaa atatagaaaa tgtagaagac atacataaaa ttatagagag
     2461 tttaaaatta gcttttgcag attctaaaac atatgtaact gatatagagc atatgaaggt
     2521 aaagattcag gaattactaa gccaagagta tgccaaaaaa agaagtttat taattgacaa
     2581 taaagaggct ttatatccaa ctgcaggaga gccatattgt ggtggtacag tatacctttg
     2641 tactgcagat aagtatggaa atatggtttc atatatacag agcaattaca taaattttgg
     2701 ttcaggaatt gtgataccaa gaactgggat tgctttacat agtagaggaa ataattttaa
     2761 tttagaccct aaacaccaca atgtagtaaa accatttaaa aaaccttatc atactataat
     2821 acctggattt ttaggtaaag aagataaagc aataggtcca tttggtgtaa tgggagcttt
     2881 tatgcaacca caaggacata ttcaagtgtt aacaaatatg attgattttg gattaaatcc
     2941 acaagaggcg ctagatgctc caagatggca gtggataaaa ggaaaagaaa tagaagttga
     3001 accagagatg ccaaagcata taatagattc attaattgaa aagggtcatg aaataaaagt
     3061 aattcatgat actgtcgaca tgggacgagg tcaaataata tttaaaacag aacaggaaag
     3121 ttatatttgt gggacggaat caagatgtga tggtcatatt gcagtttatt aatattatca
     3181 actaaaatat atataataaa tttaaagagt atatgaaata ataagtaaag gcgaaagtct
     3241 acaatgactg acgcctttac aaaacttaag ctatcatatg ctgattttat atagattatg
     3301 agccttattc ttgaccagtt ttgatttcat tgtcatcata actaatccag tcactaaagc
     3361 taccaatata aatcttgtga ggtatatcca gttctctaag agcaagactg ttaacacaag
     3421 cagatatccc tgaaccacaa gataaaatta cttcatcata attatttaaa tctttaaaga
     3481 atttttttaa aaattctata tctttaagtg agccattttc aaagtcactt tttattaaat
     3541 ccttacaaaa ataatttttt gcacttggta tatgaccagc ttttgagtaa gcaggttcaa
     3601 ctaaaccctg gtatctctca ttagcacgac aatctattat tacagtatct tttttgtata
     3661 atttagactt aacatattcc attggaacaa ctaaatcatt gtttatattt ggtctaattt
     3721 ccttacctgt acaacttggt atattcactt tctcttccaa ttctccacct tcctttacga
     3781 aagatgttat tcccccatct agtacataga catttttaag ccccaaatgt tttaattgga
     3841 aaaataatct gcaagctcca tttaaatctc catcatcata agttacaatc gtagtgtcat
     3901 tatcaacacc cattttttct aatttttctt ttagtattaa agggtcttgt aaaggatttt
     3961 taccaccatg ttcttttgta ggagatgata aatccttgtc tatatctaag ataaaactgc
     4021 ctttgatgtg tcctttttta taagaatcaa taccataagt tctattgata agatcaaatc
     4081 tacaatctat tacaactaga ttatcatttt tttctagttt tgatataagt tcttttgctg
     4141 aaataatgtt tttcaaagaa tgtacctcct gtgtttatat tatctttatt ttaatttata
     4201 ttaattatta actttataaa ctaatattat tataatttat tacataagta aataaatctt
     4261 tagaaattgt aaaaatttta tataaaaatt caaataatta atgaatattg aaaattttag
     4321 atatataata aaataagagt atattttata caatatgaaa gctggtattg caatagatta
     4381 aagccttata cacgtttagt ttttaaagaa attagtgtta ttatgaaaac ctaagttaat
     4441 ttattaaata aataattgaa taaaaaaatt ttattatgta tttataaaat ttttgttaca
     4501 aatttggcat aatatagtat actttaagtg tgtattaggt taaatctata acaaattaga
     4561 cgtatgatat agacttagtg tttattggca tttacaagaa atttgttaat atcaactatt
     4621 tgtagtttgg gcagttataa ttattatagg ggggatgtcc aatacaaaac aatcaaccaa
     4681 gaactaatat tatattataa agagttatag ttttgtgtgt ttatgttttt aattaaattt
     4741 aaagtgggaa aaatatattt gttagaatgg agaatagcga gatgagtaat aaaatagtta
     4801 gtaagagaca attaacagat agtatttatt taatggaaat agaggcacct agagttgcaa
     4861 aatcttctca accaggtcaa tttataataa ttaaaaatga tgaaaaagga gagaggattc
     4921 ctcttacaat agctgactat gatagagaaa aaggaacagt aactattgta ttccaaacag
     4981 ttggtgcttc aactaaaaag ttagcaatgt ttgaagaaaa cgattttgta atggattttg
     5041 tagggccatt aggacaagca agtgaattta tacatgaaga tatagaagaa ttaagaaata
     5101 aaaagatatt atttgtagct ggtggagttg gttctgctcc agtttatcca caagttaaat
     5161 ggtttaaaga gcatggatta gatgtagatg taataattgg tgcaagaact aaagaattaa
     5221 ttatattaga agatgatatg aaaaaagtag ctaaaaatgt atatgtatca acagacgatg
     5281 gtacatatgg atttaatgga agagttacag acctattaaa agatttagtt gataatcaag
     5341 gtaaaaaata cgaccaagca atagtaattg gaccaatgat aatgatgaaa tttatgtgcc
     5401 aattaactaa agaactaaat atacctacta tagtaagttt aaatacaata atgatagatg
     5461 gaacaggtat gtgtggtggc tgtagagtta gtgttggaaa tgaaactaag tttgcatgtg
     5521 tagatggtcc tgaatttgat ggtcatttag ttgattttga tcaagcaatg agaagacagt
     5581 ctatgtacaa gactcaagag ggaagagcta tgctaaaact tgaagaaggc gatagtcatc
     5641 atcatagtaa ttgtggatgt ggaggtaaca aataatggat gctaaaaaag taaaagtacc
     5701 agttagagaa caagaacctg ctgtgagagc aacaaatttt gatgaagtat gcttagggta
     5761 taataaagaa gaagctatgg cagaagctaa tagatgttta gcttgtaaaa aaccaaaatg
     5821 tgtgggtgga tgtccagtag gaatcgacat accagggttt ataacaaaaa ttaaagaaga
     5881 tgatatagaa ggtgctgcta aagtaatagc taagagcagt tcattaccag ctgtttgtgg
     5941 tagagtatgc ccacaagaaa gtcaatgtga aggtgtatgt atacttggaa taaaatcaga
     6001 tgctgtatca ataggtaaat tagaaagatt tgtagcagat tggtcaaaag aaaatgacat
     6061 aaatttatct gatacagagc ctaagaaaaa tcaaaaagta gcagtaatag gaagtggtcc
     6121 agcaggtcta gcttgtgctg gtgatttagc taaaaaaggc tatgatgtaa caatctttga
     6181 agcaatgcat gagccaggtg gagttttaac ttacggaata cctgagttta gacttccaaa
     6241 acaagctgtt gttcaacctg aaatagacaa tataagaaaa ttaggtgtaa aaatagaaac
     6301 taatgtaatt gttggtaaaa caattacagt agatgaactt atagaagatg aaggatttga
     6361 agctatattc ataggttcag gagctggtct tccaatgttt atgaatatac caggggaaaa
     6421 tgcaaatgga gtattttctg caaatgagtt tttaactaga gtaaacttaa tgaaggctta
     6481 tagagatgat tatgatacac ctattagttc tggtaaaaaa gttgctgtag taggtggtgg
     6541 aaatgttgct atggacgctg ctagaactgc attaagatta ggttcagaat catatatagt
     6601 ttatagaaga agtgaaaaag agcttccagc aagagctgaa gaagttcatc atgcaaaaga
     6661 agaaggcatt atatttaata cattaacaaa tcctaaggaa attttagtag atgaaaatgg
     6721 atatgttaaa ggtatggttt gtataagaat ggaattagga gagccagatg attctggaag
     6781 aagaagacct atagaaatag aaggttctga atttgtactt gatgtagaca cagttataat
     6841 gtcacttgga acaagcccaa atccactaat atcttctaca acaaagagtc ttgatataaa
     6901 taagaagaga tgcttaataa cagatgaaaa tggtcagact tctaaagagg gtgtatttgc
     6961 aggtggagat gctgttactg gagcagcaac agtaatatct gcaatgggtg ctggtaaaac
     7021 agcagcagca tcaatagatg aatatttaaa agcaaaagtt aatgcttaat ttgtaaatta
     7081 aagagagggt gtctcaaaat gaacttttta gttcatgagg cattcttttt gtacaaaatc
     7141 aaatatgttt taatcatttc aacaatgaaa atttcattcc tgtagaaagt ctaactttta
     7201 ggtcttagta caatctctat tttgatgtag ccactttttt agatggttta ataatttatc
     7261 agttattatt gtaatatttg atactaagta acataacttt gtctaactat gataaaatag
     7321 ttatacaata aaggtcttgt aaggagtgaa taatgtgtgg ctaataaaat cagatttgtc
     7381 aaatctaaca ttaaataata gaaaaataaa ttgtgtaatt aatggtattt taatatgaaa
     7441 aaaagagaaa aagtatttga tgggctagtt tttaagcata tattaatctt attaatacta
     7501 ttagttttga ttgatttaac aagtaccatc atatatgcaa gtaactctat ggaaattact
     7561 tctagaaata tgattgaggc atcaaaaaga gaacttgaaa actatttaga agtaaacata
     7621 tctttattaa aagctcttag tcaagatgat agattttcta atggtgaaac atcactaatt
     7681 gaaaagggaa aattattaag tccatatcaa aaggaatata atctttttat gattggtata
     7741 acagatacaa aaggaaatgc ttcaagtaca tatagggaaa atgttaactc aataaaagat
     7801 aagcctagtt ttgagaaagc aattaaaaca aagcaagtag tagtttcaga tattgaagtc
     7861 agtaatgtaa caggtgacaa agtatttatc atatatgtac caattgtaaa aaataatgag
     7921 atgattggga ccatttttgc ttctttttat tttcaagatg taaataattt aataagtaga
     7981 agcaattttg atgattctat taaatttcta atgatagata aagattatac tattatttct
     8041 catccaaaca aaaaatatgt aaatgataaa agtaagatgc ttgatttaga agggaatatt
     8101 ataggaacca ctaaaagtga aatcttaaaa aatataaata aaaaatgtca aggtgatttt
     8161 ttatcttggg ataattggag attatataat gtaaaatata cgaacataaa gtggactaat
     8221 tggactttag tatcaaaatg taatatattt aagaacttta aaaatctaat agttaatttt
     8281 atgataaagt tgtattttta tatagtaata tttatgatat tgtggaaatt aagtaatgct
     8341 aaattaatag aacaacttaa aaaattggct tattatgatt ctttaagtgg aataaaaaat
     8401 aaagaaaagt ttagaaaa
//
LOCUS       DAEMHW010000163         8252 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEMHW010000163 DAEMHW010000000
VERSION     DAEMHW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.163"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..1282
                     /locus_tag="KPK67_002981"
     CDS             92..1282
                     /locus_tag="KPK67_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2459915.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            1452..2414
                     /locus_tag="KPK67_002982"
     CDS             1452..2414
                     /locus_tag="KPK67_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG2459916.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(2536..3198)
                     /gene="zmp1"
                     /locus_tag="KPK67_002983"
     CDS             complement(2536..3198)
                     /gene="zmp1"
                     /locus_tag="KPK67_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBG2459917.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKLACPQTYSFLQNL
                     AK"
     regulatory      complement(3298..3383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3569..6487)
                     /locus_tag="KPK67_002984"
     CDS             complement(3569..6487)
                     /locus_tag="KPK67_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG2459918.1"
                     /translation="MKKGNRKTLLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSVLEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDANFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSVKNPGTYYLVEIMAPRGY
                     EKLKDKVKVEIGNEDIGKIVDIGNIENLKQENPPVNPPIPPDTDEPMVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPVPPSEDIINPPIPEVLNPPVPPSEEMIETPDKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(6605..6690)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(6817..7995)
                     /locus_tag="KPK67_002985"
     CDS             complement(6817..7995)
                     /locus_tag="KPK67_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG2459919.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
BASE COUNT         2592 a         1310 c         1009 g         3341 t
ORIGIN      
        1 aacaagtaat ttttcgaaaa ttgtctaaat tcagtaaaaa tatggtaata tatatataag
       61 caatataaat ctataaaaag gagtgttaca tatgatatct aataaaatgc aaactttagt
      121 tgcaaatagt tctgttataa gagctatgtt tgaagaagga aagaaacttt ctgatattta
      181 tggtgaggaa aatgtttttg attttagtat aggaaatcca agtgttgaac ctccagagac
      241 tattaaagca gttataaatg atattttaaa tgaagaatct ccaaatttag ttcatggata
      301 tatgaacaac tcaggatatg aggatgtaag agatgctatt gctgaacaca taaataaaaa
      361 agatggatta aatcttacaa gagaaaattt aattatgact tgtggtgctg ctggtggtct
      421 aaatattatt ttaaaaactc ttttaaatcc tggtgatgaa gttattgctt ttgcaccgta
      481 ttttggtgag tataaaaatt atacagaaaa ctatgatgga aaattaatag aagttcctac
      541 aaatatagag acttttgagc cagatttaga tgctctaaaa aatgctatta ctccaaaaac
      601 aagagcttta ataataaaca ctccaaataa tcctactggt gtcatatatt ctgaagaatt
      661 attaaaaaat cttggagaat tattggattc aaaacaaaaa gaatttaata caagcatata
      721 cctaatttct gatgagccat atagagaaat aatttatgat ggtgccaaag ttccttgtgt
      781 actaaaatac tatagaaatt catttatagg atactcttat agtaaatcat tatctctacc
      841 tggtgaacgt ataggatata ttgttgctaa tgggcaaatg gatgattttg atgatgttat
      901 gtcttcatta aatgttgcaa atagaatact tggatttgta aatgctcctt ctctttttca
      961 aagggttatt gcaagaagtt tagatgcaga agttgatgta aatatttata aaaagaattt
     1021 tgaccttcta tataatagtt taatcgatat gggatattct tgtgttaaac ctaatggtac
     1081 attctatttg ttcccaaaag ctccaattga agatgataaa aaattctgca atgatgcaaa
     1141 acaatttaat ttattattag taccaggttc ttcttttggt tgtccaggtc attttagagt
     1201 atcatattgt gtatcttatg ataaagttaa aagttcttta ccagcatttg aaaaattagc
     1261 caaattatat aatttaaaat aactaaatca aagtaattca aataattaac taccttagta
     1321 tagaatactt acattctata ttaaggtagt ttataatttt atgtactata ttttatacat
     1381 accatattat tccaatatgc ttgaatattc ttatctatag tactaatata aattacaagg
     1441 aggtgtttga cttgaaaaaa ggttatgttt atataatact gactacctta ttttttagtt
     1501 caatggaaat tagtctaaaa actgtcacta atgactttaa tcccttacaa ataactttat
     1561 caagattttt tgttggcggt ttagtcttac taccttttgc tgtaaaaaga ttaagagctc
     1621 ttagtctttc tattacaaaa tcagacttaa aatactttgc atttttaggt tttatgtgtg
     1681 tagtagtaag tatgtcattg tatcaacttg ctgttctaaa cacaaaggca tctgtagttg
     1741 ctgttttgtt cagttgtaat ccagtctttg taatgctttt agcttactta attttgaagg
     1801 aaaaaatata taggcacaat gtaatttcac ttatattaga agttcttgga attatagtta
     1861 taataaatcc attgcataca aaactaactc ttagtggaat attcttaaca ttatcttctg
     1921 ctattatttt ttcaatgtat actgtatttg gtaaaagaaa aaccttaaaa tttggtggaa
     1981 tagttgtaac ctgctttagc tttatatttg gtagtttgga aatgttgata ttggttttac
     2041 tcactaaaat taattttatt gcagatatat taaataaaaa taagcttcat gtatttgcaa
     2101 atatccctat ttttagtgga tataccttac acaatatgcc cattatgatt tatgtattcg
     2161 tatttgttac aggtgttggt tatgcgcttt actttatggc aatggaagct acatctacta
     2221 gtcttacatc tttagtattc ttctttaaac cagtaatatc tccaatacta gcattattaa
     2281 tacttaaaga aattatccct ataaacatgg ttataggaat tttattgata gtaattggtt
     2341 caataatttc tattatacct acgataatta ctcaaatgca taataaacac gttgaagcct
     2401 tgtataagga gtagtaattg ataatctatc atagttatac ataagacaaa atttattaga
     2461 tttaaacgca atataaaaga gatgctatta taatagcatc tctttattta tatttatttt
     2521 aaatttattg aaactctatt tagctaaatt ttgcaaaaag ctataagttt gtggacaagc
     2581 taattttaac ttactatttg tatcttgatt taaatagtaa tatgcaaatg attctgcaaa
     2641 aaattcttct ggatatacac ctaagtagtt tacattacct aaagaacgac cttctttagc
     2701 aaatatttgt ttgaattgtg ctgatttaga tatatcattt aatactatat ggtctattgc
     2761 atgtgctgtt tcatgtaatt ctaaatttat tgcatcatgc cctttacctt tgttactaaa
     2821 tcctattcta agagctactg tacttccacc taaacctgga acatcatccc aagtttttcc
     2881 agtaccttcc catcccttag gaactacacc ttttaaataa gcatattctt tttcatctgt
     2941 taactttcca cttaataatt ttatttttaa gttattttgc tttaatgcat ttaaatactt
     3001 tccatctata ttagctaatc tatttaccat agcattagct tcgtttttat cgtaatttcc
     3061 agttggaaaa actactattt gactaagtgt gtctttattt tgttgtatag tagtactatc
     3121 tgcatgtgca cttactggaa ctgaacttat taagaatatt gatattatag ctattaataa
     3181 ttttttactt ggtctcatat tattccctcc tttgagttag gccgggagta ataaatttca
     3241 aaataaaaaa gatacccaag agtatctttg tatatacaaa aagaaatttt tactcttttc
     3301 ggcaactggc tggcatggtt tactaaactt tagcccctat agctttgcgt gactaggttt
     3361 ccctagtttt gcctcattta attttatatc tatattatag cttatatctt tttaattgtc
     3421 tatatccctt tggaactttt ttctaaaaag ttattatttt tttacataaa tcttcaaaaa
     3481 aaaatatctt ctaacaatat tttcattatt aaatattgtt ttagaagata ttttttaata
     3541 taacttatat tttttataat gttagggtct aatttgtatt tttatttctt cttaatacga
     3601 taagtcctac aatagctcct ataaccaaaa gtatttctcc aataattgta gtactatccc
     3661 ctgtttttgg tggtactggt ggatttacca aagtatcatc tttaacttta ttattttttt
     3721 cttctgacac tgatggcttt actacttctg gtattggaat aatctgttta tcaggagttt
     3781 ctatcatctc ttctgatggt ggtactggcg gattcaatac ctctggtatt ggtggattta
     3841 ttatatcttc tgatggtggt actggtggat ttataactat agtatcctct gtatcagatg
     3901 aacttgatgg tttccttggt ttatctgtgc ttggtggtac tggtggattt accattggtt
     3961 catctgtatc tggaggtatt ggtggattta ctggtggatt ttcttgtttt agattttcta
     4021 tatttccaat atctactatc ttaccaatat cttcattacc aatctcaacc ttaactttat
     4081 ccttcagttt ttcatatcca cgtggagcca ttatctccac taaataatat gttcctgggt
     4141 tttttacact agattgaatt ataccatctt catcactaac taaatcctta actactatat
     4201 ttccaaatgc atcttctaaa ttgaacttag cacctgaaag tacattttta tctaaagcat
     4261 ccactttttt agcttcaaaa gcataacctt gcttagtagt ctctactcct ggaggacttg
     4321 aaccattaat ctgagccaca tttcgtatag tctccccaac ttttacattt gtgaagtttg
     4381 catcaaatgt aacccaactt tgcacaggtt ccccttcttt tatctgttta aattcgtatt
     4441 tatttactaa tcttattgta ttttttccat ctccagcttc ttttttaagt tcaagattat
     4501 cagaatattt tatattagat atttctagaa ctgagtttac ttcatctaaa agatttactt
     4561 gtcctgcatc ataaactcca taagttgaca tattgatttt gtatttaaca atctggttat
     4621 cactactgtt taaaactttt ttatcaactt ctttttttgc gcttattaat ccagaatttt
     4681 tcttaaagtt tatctcatca tttttatcaa tttcactatt atttgcatca agaacagctc
     4741 tatttatata gctatcttca atactaatta cctttaatgt ataagctata aaaaactttg
     4801 attccacttc ttctttactg agattaattg taaatccagt tataacttta tctacttgcc
     4861 cactttcatt tcttttttct tctactttat atgaaatatt attggcaatt ttttctttac
     4921 tccaaggagt ccagctccca ttcattggaa tttctacaac tctaaaatca tgcccagctt
     4981 ctcctgaagt agttaatctc atgccttctg gtatattatc tgttataaca acattactta
     5041 ttggtgattt atcatcattt attgaaatct gatagtttat tacttgacca acttgagtaa
     5101 tatttgttgc tcctgaatgt cctttagtaa gaagataatc atctcctcta tcaaatatac
     5161 ttacagtagc atcatcttct tgtatcttct taccatcaga ttcaatccta gctacattct
     5221 tatatttatt accatgtttt gcatttttgt cactgattct agccttatac ttaacagtat
     5281 acgatttatc tatatttcca aaattaactg aaatagaatt tttactctgt tcaatattaa
     5341 aatcactaac ttcttttcca tcacttgtta ctacacttac agaaccattt atcaatgtca
     5401 atccttcagg aagctcatca tacaaaataa tatcttttaa ctcttcctgc ttttcattta
     5461 cttgtatact gtaatttata tctttatttt taactggcat aaatacagta gttgattttt
     5521 ttccttcaac aagcttttta agtgttcctt tagcatctgg aacatccgtt ttatctccag
     5581 gttttatttc tacatctata tctccacttc cactttctat ttttccagta gtttcaatat
     5641 gtatttttgc attagtatct tcatcaacct ttttaacata tgcttccaaa cctattttac
     5701 cttgcattac actttggcca ttaggtaatt ctctaaatgt aagtgtatac tctccattac
     5761 tttctgtttt atcaaagcat tcacttgtat aatctaaatt aactttattg aatacatctg
     5821 gcaccttaaa agttattgaa tctcctgctt gtattccttt tgatgttgga gtataagtta
     5881 aattaaatga tatttttttc attgattccg tttctattgt attgttatta atttttacaa
     5941 caaatccata gtcttcattt tcttcattaa gttcttcttc taaaactttc aataaatttt
     6001 catcttctaa tagattatct tcctctggag ttttttctgt atttttattg tctagtggtt
     6061 tttcatcttc taaagcttgt tctgactttt tattatctgt tgacttatct tcttctggta
     6121 tctgctctgg ctttttatta tctattggct tttctccttc tggagtttgc tctggctttt
     6181 tattatctgt tgacttatct tcttctgggg tctgttctgg ttttttatta tctgttggtt
     6241 tttcttcctc tggggtctgt tctggttttt tattatctgt tgccttatct ttctctggag
     6301 tctgttctgg ctttttattg tcgctttcct tctcctctcc agttgatgaa ttagttgaac
     6361 cactttgaat ctgactattc tctcctaatt ctgaagcata agatacagtt ggatatattg
     6421 tagacaccac catacttaaa atcataatta aactaattaa taatgtcttt ctatttcctt
     6481 tcttcatgta aatctcaccc ctttaggtta cctataattt ataatctaat cttccattaa
     6541 tattgtatat aaagtaattt ttactaaaaa attatacaaa aaaagctatg aaaataaata
     6601 aatttatatt catagccggt tgcaccactg actcagtata tccataaggt gcccaataat
     6661 agaatatact tcaacatctc tataatattt cataaaaaca attaaaattg ctttatttat
     6721 attaaaaata caattatata cttctattat attatgcctc tatactattg cctatccttt
     6781 ttacattttt taccttatga tgagattaat taataattaa ataaaatcct tatctacaac
     6841 aattacacaa ttatattcag gatttatctc cttaactaat tttgttatat cttcttttaa
     6901 gtcatcttct gacatgtttt tggaaaattc ggaaggatta actactatat caaatattaa
     6961 attcttcttg cttccttttc caactattct aaaatcatgc atagatttta caagactatt
     7021 tttcttaagt atttcttcaa gttctttttt ggctgacata acttctttat cttcaagaca
     7081 tattgggtcc atgtgaatta ctagatatat atctaatttt tcagatattt ctctctctgc
     7141 agtatctata atttcatgta tttccattat atctatattg gatggaattt ctgcatgtat
     7201 agatgcaata catctaccaa ctccatagtt atgaataatt aaatcatgtg tacctattat
     7261 atatttataa gataacaata gctctataat agaatttacc atttcttcat ccggtgcttc
     7321 ccctaaaaga ggatttatag tatcttttac aagagagaaa ccagcataaa gtatcactaa
     7381 tgatactacg ataccaacat atccatctat aggaaaatta gtaaatctag ctacaataaa
     7441 tgatataact acacaacttg aagtaaatac atctcctaaa gcatcaagtg aaactgcttt
     7501 taaggctgaa gaatcaattt tatttcccat aaatttatta aaacgactca accagatttt
     7561 aatcattata gatgcaatta aaagtataag tggtattact tcaaatttta caggaattgg
     7621 atttactatc ctttctatag atgactttac aaattgaagt ccaaccaaca tcaccataaa
     7681 tgctactatt aatgctgata aatactctat tcttccatga ccaaaagggt gttctttatc
     7741 agctggctta cttgctaatt taaaacctat catagttatt gctgaagatg ccatatcaga
     7801 aagattatta aatgcatcag ccattatagc tatactagat gtgagcattc ctatgaatac
     7861 ctttatcaca aataaaagta aattggataa tatacctact acaccagcta cataaccata
     7921 cttatttcta acatctgtat tttgaacgtt ttcgctatct ctaataaatg ttttgactag
     7981 gattttagaa aacatgaaac ttacctccct gccctttatt ttattaacaa attttataat
     8041 ctatctcttt atttatgata acataattta tatataaaat tcttaaaaat cactctactt
     8101 cagtaaaata ttattctata tatctattta gatacgaatt ttatatttcg taaagttttt
     8161 atggcttagt gtctgtttaa tagattctat ataagttttt acaaagatta tttattatga
     8221 taatatcatt tttcccaagt attacttaga at
//
LOCUS       DAEMHW010000164         8201 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEMHW010000164 DAEMHW010000000
VERSION     DAEMHW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8201
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.164"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            60..950
                     /locus_tag="KPK67_002986"
     CDS             60..950
                     /locus_tag="KPK67_002986"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBG2459920.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            934..1614
                     /locus_tag="KPK67_002987"
     CDS             934..1614
                     /locus_tag="KPK67_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2459921.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            1759..2418
                     /gene="spoIIR"
                     /locus_tag="KPK67_002988"
     CDS             1759..2418
                     /gene="spoIIR"
                     /locus_tag="KPK67_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBG2459922.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            2469..2954
                     /locus_tag="KPK67_002989"
     CDS             2469..2954
                     /locus_tag="KPK67_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBG2459923.1"
                     /translation="MATLLALTFIFMPIKEVFAYAPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            3156..3962
                     /locus_tag="KPK67_002990"
     CDS             3156..3962
                     /locus_tag="KPK67_002990"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBG2459924.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            3979..4407
                     /locus_tag="KPK67_002991"
     CDS             3979..4407
                     /locus_tag="KPK67_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBG2459925.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            4590..5978
                     /gene="tilS"
                     /locus_tag="KPK67_002992"
     CDS             4590..5978
                     /gene="tilS"
                     /locus_tag="KPK67_002992"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBG2459926.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEVEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            6012..7982
                     /gene="ftsH"
                     /locus_tag="KPK67_002993"
     CDS             6012..7982
                     /gene="ftsH"
                     /locus_tag="KPK67_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG2459927.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
BASE COUNT         3404 a          811 c         1551 g         2435 t
ORIGIN      
        1 ttttttgtag tataatatta ttgttagtga tgcattattc tttaaacaag gaggatagaa
       61 tggattttat aagactaaag agtagagcaa aaataaattt atccattgat gtcttgggaa
      121 aaaggcaaga cggttatcat tttgttgaaa tgataatgca aacgatagat ttatacgata
      181 tagtaaagat aaaagaatta gacgaagatg aaataaaggt aaaaagcact agtttagata
      241 ttcctttaga tgaagataat attgtttata aagctgccaa aatacttaaa aataaatttt
      301 atataaaaaa aggtgtagaa atattcatag aaaaaaatat tcctgtagca gcagggatgg
      361 ctggaggaag ttctaatgca gccgcagtat tagtaggttt aaatcaccta tgggaactta
      421 gattatctga agatgaatta aaagaaatag gacttaattt gggcgcagat gttccttttt
      481 gtatatcagg aagacctgcc ttagctcaag gtattggaga aaagctaaca aacatcaaag
      541 gtttaccatg tgatacaaat atattgatat gcaaaccaga cttattcgtt tctactaaag
      601 aagtttatca aggattagat ttgaataata ttaaaaaaag accaaataat aaatatttga
      661 tagagtgttt aaaatctgaa gatataaaag ctgtatcaga aagcatggta aatattcttg
      721 aaaatgttac tataggtaaa cacaaagaaa taagtgatat aaaacaagta atgatgaaaa
      781 ataatgcttt aggttctatg atgagtggaa gtggtcctac agtttttgga ctatttaaaa
      841 ataaagaaga tgctctaatt ggaaaaaaag aattattaaa aaaatataaa caggtgtatg
      901 ttgttaatag cagtcaaaaa ggggttgaaa tttgtggaga atttaactaa actgaacttg
      961 gataattaca agccattaag agatgtagtc tttgagaatt taagagaagc aatcttagaa
     1021 ggaaaattaa agcctggtca aaggctaatg gaagtgcaac tagcagaaca attaggagta
     1081 agtaggactc cagtgagaga ggcaataaga aagcttgaat tagaaggttt agtcgttatg
     1141 ttgcctagaa aaggtgcgta tgtggcaaat atgtcattaa aggatattat ggatgtatta
     1201 gaagtaagag caagcctaga aggcttagca gcatatcttg ccgctgaaag aattagtgat
     1261 gaagatatta aaaagcttaa agatatatca gaagaattta aaaaaagcac tttggaatcc
     1321 gatgtagatg cgttattaaa acttgatgta gaatttcatg aatgtatatt taaagcaact
     1381 aataataaaa aattaatcca attaataaac tcattgtggg aacaagtata tagatttaga
     1441 gttacctata tgtctgctta tgatacatca cataatattg ttgaagagca taaatattta
     1501 cttgatgcta taattaaagg tgatagtgag ctatccaaga agtatgctac agagcatata
     1561 gaaaaagcag agcagtttat gatagataag gctatgatta gtaaagactt ataaaatata
     1621 aggtttaaaa agctatttta aaatgattgt aatcatttta agatagcttt ttttattagt
     1681 attaaaaaat attatgaata aattaaattt aattggacat aattgatgat ataaatttat
     1741 cttgaggggg aattttcaat gagaaaaatt aagattagat tgggactatt aatattaagt
     1801 cttatatcag tcatatcaat aatgacaata gtaataaatg gtgaggtaaa aaaagtagac
     1861 aatatatcaa aagattataa agataaatta ataagatttc atgttatagc aaacagcaat
     1921 acagatgaag accaagaact taaactaaaa gttagagatg aagtaataaa atatttacag
     1981 ccaaagcttc aaaattcaaa aagtataaaa gaaagtgaag ctattataaa aaaggaatat
     2041 agtagtctgg aagaaataag taaaaatatt attcttaaaa atggatataa ttacagtgta
     2101 aaggtaggta tacaatatag caatttccct actaaacaat attctaatat agttcttcca
     2161 gctggtgaat ataaagcatt aaagattatc attgggaaag gtgaaggaaa aaactggtgg
     2221 tgtgttatgt tccctccact atgttttgtt gatgaatcta atggagtgat agataaatct
     2281 acagatgata agttgaagga agttttaact gataaagaat ataagttaat aaaacaagat
     2341 acaccaaaaa aaacaagtag agtaaaaatt aagtttaaag tacttgaagt agtcaaggat
     2401 ttagagaaaa aattttaaaa ataagaatgt aaggagtggt tttaatcatg gacaggtgga
     2461 gtaaactgat ggctacacta ttagctttga cttttatatt tatgcctatt aaagaggtat
     2521 ttgcatatgc accaacagaa aatattaaga aagtaaacaa acaagaaaaa aatatagcaa
     2581 agaaagaagt aataaattta tctgataatg acttgatgtt attatctaag ttggttgcag
     2641 gagaagctag aggagaaagc tatgagggac aggttgctgt agcagctgta gtaataaata
     2701 gagtattgga ccctagattt ccagacacaa tagaagaagt catatatcaa aaaaatgctt
     2761 tctcagttgt gcttgatggt tcaataaaga tggcaccaac agactcagca cgtaaagcag
     2821 ctcaggaagc tctttatgga actgacccaa ctaataaagc agtatatttt tggaatccag
     2881 aaatagccac ttgcaaatgg ataaatagat tgaatcctta tatgagaata ggaaatcatg
     2941 tgtttgcaaa ataaatttaa atttttaagt agtgaaagga gttgtctttt tgtagaaatt
     3001 attttctaat ttaagatagc tcctttattt tttgtgtaaa ataaactata ataatcattt
     3061 aggtaataat atattaatta tgaatataaa tataaatttt agcgataaat aagttgttgt
     3121 taaacacaaa aatgtaaagt aggtgttgta taaagatgaa caataaacca ataggagttt
     3181 ttgattcggg attaggtgga ttaacagttc taaaagagat aatgaagata ttaccaaatg
     3241 aggatataat atactttgga gatactgcca gaataccata tggttcaaga tccaaagaaa
     3301 ctataattaa atatactttt caagctataa actttttaaa aacaaaaggt gtaaaagcta
     3361 tagttatagc atgtaacact gctactgcaa gaagcttaaa agaagctcaa gaaaaatatg
     3421 atataccgat aattggagtt atagaagcag gagctagaac agctgtaagt tctacaaaaa
     3481 acaaaattgt aggaataata ggaactgaag gaactataag ttcaaaagca tacaacttgg
     3541 aaatatctaa aatagatgag agtatagaaa ttgttaataa agcatgtcct ttatttgtac
     3601 ctattgttga agaaggatgg gcaaatacag aagtggctaa attgacagca aagatatatt
     3661 tacaagaatt gaaagaaaaa aatatagatt ctttagtgtt aggatgtact cattatccta
     3721 ttttaaagag aacaataggt gaagaagttg gagagcatat aaaacttgtt aatccagcta
     3781 aagagactgc aaaggattta aaaaagattt tagaggtgca aaatattata aacaatacag
     3841 aaattcatgg aacttatcaa tattatgtat cagatatacc tgaaaaattt tcagatatag
     3901 caagagagtt cttaaagaaa aaaattgata agattcaaaa agtagaaata caaaaatact
     3961 agtttatatg aagaatctat ggaggaaaaa tcgttgagtg taaaaataag tataaatact
     4021 agacaatatg atgaaaaagg aaatatggac accatagaga tgactgcctt tggtaaaatt
     4081 ttttataaaa atgatggaat atatgttatt tacaaggaaa aagaagaaga tatagaaata
     4141 actaatacta taaagatact taaaaatcaa gttagtatca aaaagtttgg tgctattaac
     4201 tcaacaatgc tgtttaaatg tggagaaagt agtactacaa agtatgtgac cccacaaggt
     4261 actttattaa ttgatattga tacaagagaa ttagatataa atatacaaga aggggaacac
     4321 ataaaattga aaatagacta taatataaag gtacaagatt tatttttagg taggaataaa
     4381 atagatatat acataggtac taaataataa ctatggctat tttccagaaa ttttataaag
     4441 gtagtgaaaa aactaccttt tatttaattt atttggtatt attatattta tattaaactt
     4501 taatttctta aaattgatac aagggtttag cgttttttta tagaaagtat acttaaaagt
     4561 gaataaaact aagtgtaaga gggataaata tgatatttga taaggtacta agtactataa
     4621 ataaacataa tttaatacaa aaaggcgata aaatagtttt aggtctttct ggaggacctg
     4681 attcagtttg tttattgcat gtactaaata gattaaaaaa agattttaat atagaaatat
     4741 atgcagcaca tttaaaccat caaataagag ggatagaagc tcaaaaagat gcattatatg
     4801 tatctaaact ttgtgaggat atgggaatta tattctttgt aaagtctatt aatgtaccaa
     4861 aatattgtga aaatgaggga ttatcattag aagaaggagc aaggaagcta agatatgaaa
     4921 tgttttatga aattaaggat aagattaaag ccaataaaat agcaataggt cataatctca
     4981 atgaccaagc cgaaactgtt atgatgcgta tcatgagagg aacaggttta aaaggcttaa
     5041 agggaattga ttatattaga gataattgca taattagacc catattagac gtggaaagaa
     5101 atgaggtaga ggagtattgt gaagcttata atttaaatcc gaggatagat aaaacaaatt
     5161 tagaaaatat atacactaga aataagataa ggttagacct tttaccatat atgaaggata
     5221 attttaattc aaatgtaata gaatctatag tgaggatgag caatagttta aaaagtgata
     5281 acgattatat tgagaaagag gcagaagcta aatttagaga agtttcaaat ataaaagaaa
     5341 aaggctttgt agagataaat ttagacgatt ttgtttgctt acatgatgct atcaaagtta
     5401 gagttcttag aaattccata aaacatatac taggagatac taattttgtt gaccaaagac
     5461 atatagaaga tataatgtct ttagaagata attcaaaagt aaataaaatg ttaactcttc
     5521 caagaaatat atttgtttat agaaaaaaag acagtataat attaacgaat gaggaaattg
     5581 ttaatgagga aattgaattt tattacaatg tacctagtaa tgggtttatt aaaataaaag
     5641 aattaaaaca aattattgag acccaagtaa tgagcataga taggtataag agtatgaaat
     5701 tagacaattc atctaaaggg tttgatttta ataaggtaaa agggggtata gtaataagaa
     5761 gcagaaggca aggtgataag ataaagcttg ctatgggaag taaaaaagtg aaagatttat
     5821 tcatagattt aaaaatccca agagaagaaa gatgtaaaat tcctataatc acagatagtg
     5881 aaggaataat atgtgttgga gattataaaa tcagtgaaaa ttataaaatt gatgaaaaca
     5941 caaaagaagt attaaaaatt aattttaaca aattataggg caaagcaatg tatgaaagga
     6001 gggcttctat attgaataag ttgctaaaag gggcaggttt ttatctactt gtttttataa
     6061 ttatagtagg tatagttcaa ttctcaggta agcctacaga aaaaataaaa gatttaaaat
     6121 tctctgaagt atatagagaa ttaacagatg agaatatatc aagattatac tttgtaaatc
     6181 aaacttctgt agaaggtact ataaaagata caaatacaaa gtttaagtca tatgttccaa
     6241 cagaagtgat gggaaataaa cttgcagatg aggtgttaga ccaagctaag gctgggaaac
     6301 taacttttgg tggtgaagct aagccatcta caccttggtt tgtcgaaatg ttgcctacat
     6361 tactgttaat attcttcatg gttattattt ggttcgtgtt tatgaatcag tctcaaggtg
     6421 gaggcggaaa ggttatgagt tttggcaaat caaaagctaa ggttcataaa gatgatgaaa
     6481 aaactagagt tacatttaaa gatgtagctg gactagatga ggaaaaagaa gatttgcagg
     6541 aggtagtaga tttcttaaag aatcctaaga aatatattga attgggtgca agaataccaa
     6601 aaggaatgct tatggtagga cctcctggaa ctggtaaaac atatctatca agagcagttg
     6661 caggagaagc aggagttccc tttttcagta taagtggttc tgatttcgtt gagatgtttg
     6721 ttggggttgg tgcttcaaga gtaagagatt tatttgaaca ggctaagaaa agtgctccag
     6781 ctatcatatt catagatgaa attgatgcag ttggtagaaa aagaggtgca ggtcttggtg
     6841 gaggtcatga tgaaagagag caaactctta atcaacttct agttgaaatg gatggatttg
     6901 gggtaaatca aggtataata atcatggcag ctacaaatag accagatata cttgacccag
     6961 ctttacttag accaggaaga tttgacagac aagttgttgt tggtacacca gatgtaaaag
     7021 gtagagaggc tatatttaaa gttcactcaa gaaataaacc attaagtgat gatgtaaaaa
     7081 tggatgtctt ggctagaaga acaccaggat ttactcctgc tgatatagag aacttaatga
     7141 atgaggctgc aatattaaca gctagaaaaa gagaaaagaa aattaagatg gaaactatag
     7201 aagaagctat aacaaaggtt atagcaggtg tggctaaaaa atcgaaagta ataagtgaga
     7261 aagaaagaag acttacagca taccatgaag gagggcatgc agtttgtgcc catgtacttg
     7321 aagaagtaag tcctgttcat caagttacaa tagttccaag aggaagagct ggagggttta
     7381 caatgcaact tcctgttgaa gataagttct atgctacaaa aaatgaaatg aaagaaaata
     7441 ttgtagtttt acttggtggt agagtagcag aagagctagt acttaaggat gtatctacag
     7501 gtgcttcaaa tgacttagaa agagttacag ctactgcaag aagtatggta acaaagtatg
     7561 gaatgagttc taaacttggt ccaatgtcat ttgacagtga tgatgaagta ttcctaggta
     7621 atagtttttc aagtaagaga aattattctg aagaagttgc ttttgagata gaccaagaaa
     7681 caaagcgtat agttgatggt gcatatgata agacaagaag tatattacaa gaaaatatgg
     7741 atagattaga gtatgtagca caagctttac ttatatatga aactttagat gcagaacaat
     7801 ttgtaaaagc atttaataaa gagttacctc tgaatgaaat tgaaaatgca gttacagagg
     7861 aaaattcttc taaagaagtt gaagaacaat taactataaa attagaaaaa gatgaagaag
     7921 aaagaaacaa tgttattgat ataaataaaa atttagaaga taaatctgat aaagataaat
     7981 agttttaaga ccttggtcat tttaagatta ttatctgatg tggattaaat actaaaactg
     8041 aaagtataat attaaattaa taagcaaatt atatttgcat gtattaattt aattgatact
     8101 ttcagttttt ttattatttt aaaattattt aaataaaatg taaaattata taaatcatca
     8161 atataaaata taaaacaacc attataataa ttaaaaagga a
//
LOCUS       DAEMHW010000165         7956 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEMHW010000165 DAEMHW010000000
VERSION     DAEMHW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.165"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            341..3175
                     /locus_tag="KPK67_002994"
     CDS             341..3175
                     /locus_tag="KPK67_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2459928.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKKLSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            3218..4462
                     /locus_tag="KPK67_002995"
     CDS             3218..4462
                     /locus_tag="KPK67_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG2459929.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKV"
     gene            complement(4954..7215)
                     /locus_tag="KPK67_002996"
                     /pseudo
     CDS             complement(4954..7215)
                     /locus_tag="KPK67_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
     gene            complement(7502..7956)
                     /locus_tag="KPK67_002997"
                     /pseudo
     CDS             complement(7502..7956)
                     /locus_tag="KPK67_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
BASE COUNT         2703 a         1212 c         1070 g         2971 t
ORIGIN      
        1 aattattctt aaccttatca gttttttcat atatcttcat ctataccgac catatattaa
       61 ttacattgac catatattaa taaatgatat tacatagact taagtatatt aaaaaactta
      121 aatttagtta tttaacaaca taatactcta atttaatcta taaaaatcca tacctaatat
      181 aatgcaactc ttattttaac accatctatt acacaaaaca acatattaaa aagtaaaata
      241 caaaatatcc actataaacc ttattttaat actgaatata tattataata tatttaatgt
      301 agcttaaata tagtttaaac ataaagaaag gaaacagtac atgctaagaa aagataaagt
      361 tctattatct ctacataaat tatgtgaaaa tatgacttta aaagaccttc aatcagataa
      421 aacaggattc actacaatag aaattgctaa ccattcatct cttgatagaa gtaataccag
      481 taaagaatta aataacctat acaatgaaaa aaaagttctg aaaatatctg gtaagccaat
      541 tttatttcta catgctagta ttatagaaaa tttaattggc cgtactttga acagtgatga
      601 attttctata aataattgta atgagctact taaatcaagt aaaaatgact cagaagatat
      661 tttttcaaca ttacttggac atgattctag tcttaaacta cctatagaaa aagctaaatc
      721 tgctatactc tatcctccaa aaggtcttaa tacgttactc ataggaccta ctggtgttgg
      781 aaaaagtaca tttgctgaaa caatgtacaa atatgctgta gaaagtaaaa tcttttcttc
      841 tgaaagcaaa ttcgtagtat ttaactgtgc tgaatatgca gaaaatgcta acttattatt
      901 aagtaattta tttggttatg tcaaaggttc ttttactggt gctaataagg ataaattcgg
      961 catagttgca caggctgatg gaggcatatt attcttagat gaaatacaca gattgccacc
     1021 agaaggtcaa gagatgcttt ttcttttgat ggacaaaggc atatacagac gattaggaga
     1081 atctgacatt gtgcacaaag ctaatgtctt tattgtgtgt gccacaacag aagatataca
     1141 ttcttctctg cttgggactt ttttaagaag aatccctgtt acaatcaacc tacctgcttt
     1201 acaggatagg tctttaaaag aaagattatc tcttattaaa tacttcttta ttatagaatc
     1261 aaaatacacc aatgcaacta taaaagttca caaagatgta attaaatcat tactccttta
     1321 tgattgtttt ggtaatatag gtcagcttaa atctgacata caacttatga gtgcaagagc
     1381 cttcttaaat tacaaaactg gacttaagga taatatagaa atagatttat cccttgttcc
     1441 tgattacatc tatattggtc ttttaaaatt gaatgaaaat cgtagtaatc taaataaatt
     1501 atcagaactt gattatatgc ttttttatga atttaatggt aataacttag aagttgaata
     1561 tacaaaaaaa ctatctaaat acaatataga tacatcaaac aatggattta aatactctac
     1621 agagattcct tatctggaaa atgatataag gagtatgttt ataaattaca tggaaaagta
     1681 ctctacaaaa ttactttcga tatcttcagt aactcattct gattatacaa gtaatgagat
     1741 ttttaaagta atcaatccaa agatttacta tgctgttgaa cattctttaa aagaggctga
     1801 aaaaatatcg agaaacaaat acacaaaaca aacttatatt gctctttcaa tgcatataag
     1861 tgcacttata gaaaatataa atcaagacac atataatcct aagtacaatt tatataatga
     1921 taaaattttc ttagataaag atttaaatat agctaaaata gttttaagta taattgaaaa
     1981 tgacttacaa acatctttac ctaaagatga agtaaaattt atatccatgt ttctaaatag
     2041 catgtatctt gaaccaaaca atcaatctga aagtatagga gtcataatac ttgctcatgg
     2101 aaattcaaca gcaagcagta tgtgtgatgt agcaaattca ttgcttggta caaaccattg
     2161 taatgcaatt gacatgcctc ttgatgtaaa agtaagtacc atacttgata aaactataga
     2221 gttggtaaaa gaatgtgacg aaggtaaggg tgtacttctt ttaacagata tgggctctct
     2281 tatagctttt ggagaactta tttcagaaaa aacaaaaatc aaaactcgct caattgaaat
     2341 ggtatctact ccaatagttt tagaagcagt aagaaaaagt acactaccag aaatgacttt
     2401 ggatgaatta gctaattcct taagctcttt aaatccatat attggacgag cagcaatcga
     2461 ttcaacagaa ataaactatc ctgaaaagtc aagagagtat gtaataataa ctacttgtat
     2521 aacaggatat ggttcagcta tgaaagtagc agactttata aattcttcac ttaaaaatat
     2581 tagtaaatac aatattaatt tagttcctca taatatagaa agttttaaaa tgtataagaa
     2641 taaatttagt aatactaaaa ttctggcagt tgttggaagt gtagatttta atgtagccaa
     2701 cacccctttt atacacatag aggaactact aaatggatat ggcctagata tgctaaacaa
     2761 aataatagaa ggtacattca atgccaatag tattacaaat ataaaagaaa caaatctatc
     2821 atacaacagt ttaataataa actttatgac ccaaaactta gagcttttgg actctgttaa
     2881 attgttcaac tatgtcaatt cttccttatt tacaataata gaattaatag atgaaaactt
     2941 aaagagcaaa tttaagctag gatatatact acattgctct tttatgatag aaagatgttt
     3001 taaaaataca acttttccat ataataacat tactgaatta attagtaaaa atcatgaact
     3061 atatagttta atacgtgaag cctttaagcc aacagaagct tattttaata ttttgatacc
     3121 agacgaagaa attgcttata taatagacat gattaatgag tatctaaata aataaaattt
     3181 atagcactta ttttgtgcat attaattaat atatgaaatg gaggacgaga ttttgaaagg
     3241 aaaaataaaa cagttaacaa tacttgcatt aatttttata tttataactc ctgtatttgc
     3301 atttgcagat actccaccag taccaaattc ttcaagagca gcactactta tagaccagga
     3361 aactaaaaga atattatttg aaaaaaacat tgatgaaaaa atgccattag cgagtttaag
     3421 taaaatgatg acctttttat tggcaataga agcagttgat aaaaatcaag ttaaagagac
     3481 tgatatggtg aaaatagaca agtctacagc ttcagtagga ggttctactt gtaaacttaa
     3541 agatggtgat gaaatatctc taggagagct tatgcagggt ctaatgctag tatctggaaa
     3601 tgatgctgct atagccatag ctaaacatat tggtaaaact gaaaagaatt ttgtaaatat
     3661 gatgaacaag aaagcagaag aaataggaat gattgatacg tattatttta atccaaatgg
     3721 gcttcctata tatactgacc cagaacataa agaacctcct attgaaaata tgtcaactgc
     3781 tcacgatata gttacacttg gaaagtatat gtacgaccat tatgaaaatc aagttacaag
     3841 aataaccact atgcaagtat ataatgatac taaaaaggat tttacacatt ataacacaaa
     3901 tcctctgctt gtttcagtac ctggtgttga tggaataaag acaggatata cagataatgc
     3961 tggatattgc cttgcatttt ctatgatggt acctaaagat gctaaaaacg aaagaaatca
     4021 tagattgata ggtgttgtac ttggtgatgg taacaagaaa aatagaattt cttcttccgc
     4081 tactttattg aaatatggta aagataattt ccattctaaa aagatagcac ataagggtga
     4141 tattatagag actccttgtg ttgatggtat tgatgatttt aagataacag ttaaggttga
     4201 taaagatttg tatggtgttg tttcagataa cgaaaatatc aatcctaagg tagtgtttaa
     4261 aaatatgaat tatcctatcc ataagggaga tattgttggt gttgcaaagt attataatga
     4321 ctctggtaaa tttgttggaa gtgttgatgt aaaaagtgaa agtaatatag gatgtattcc
     4381 tttgaaggat aagattaaga ttaaggttgc taagataaat aaaaaacttg agattaaaaa
     4441 ttctgtttgt tttaaagtat agtaaagcat attattaaaa gggtatgtta aatttataaa
     4501 gactccactt tgtggagtct ttttgttttt tacaaaaaat taaaatagtg gcaacaattg
     4561 aggatgtgag ggagagttgc cactattttg tatagggtat tatattatgt ttttaagcta
     4621 gttttactat cttctatgtt tatataataa ctcttgtgtg tgaatttaat tgataattaa
     4681 tatgaaaatt atgtgaagtt ttgatattag gttatacttt gaactttatc taacatttta
     4741 aattttatat ctctaaatta tctaaaatta ctatatcaat taactttaca tgataaaaag
     4801 gctgtgtata aatttatttt taaaaatttt actcacagcc ttaaattaaa ttttctattt
     4861 aaagtatata taaattttat taaataattt gctttttatt tcttatttca taaatcctag
     4921 tattcttgat aacattaatg caattgtagt aggtactttc cctgcttcaa atgtggctgt
     4981 tgctgtgttt aatgctgtta ctgcatcatc tatttctttt tgtgttgcat ctgctttatc
     5041 taatattgct tgtgcttcat ctattgctgt tttatatgtc gcttttgatc ctacagcata
     5101 gtttcctgct gctattcctt ctgttgctcc agcatgtaat gatgttgctt ctgttactgc
     5161 atcttgtaat gctgttttat ctactgctac tacttttcct gcttcaaatg tctctgttgc
     5221 tgtgtttaat gctgataatg catcatttac ttctttctgt gttgcatcag atttgtctaa
     5281 tattgcctgt gcttcatcta ttgctgtttt atacgttgct tttgatccta cagcatattc
     5341 tccctctgct gttccttctg ttgctttagc atgtaatgct gttgctgctg ttactgcatc
     5401 ttgtaatgct gttttatcta ctgctactac ttttcctgct ttaaatgtgt ctgttgctgt
     5461 gtttaatgct gttactgcat catttacttc tttttgtgtt acaccagatt tgtctaatat
     5521 tgcctgtgct ttgtctattg ctgttttata tattgctttt gaccctactt tatattcacc
     5581 tacatttgtt ccctctattg cttcattata caatttattt gctgctgtta ttgtattttt
     5641 caaaactact ttatctactt ttgatgaaga acttcctcct ccatgtgaag agcctccact
     5701 tgatgaactt cctcctgaag agttagaact tggactattt ccacttattg ttggagaatt
     5761 atctccctta acactactta ttgtaccttt attatctata gtagtccctt tagaattatt
     5821 tgtaactgta gttatcttac caccattagt cacttttact tgacttgctc cagatgctac
     5881 tgttaaacta ccaatctcac ctttagaatt attctctatc ttagctccat ttttatcatt
     5941 aacctttaag ttagttattt ttccataatt aacaaaagtt ccatctttta catcatctat
     6001 aactacatca tccacttttg catagttatt tacatctcca ttaggcatgt tgatagttac
     6061 agtctttgta tatgtaccat ttaagtttac tgttactgcc ttatcagttt gtattgtaaa
     6121 tgcttcttta acctcactag ttggcttaaa gtttataaca tcattagcag tagctttatc
     6181 tatagctgat tttaattcag aaattgtttt agcttcaact gtttttactt cttgtaatga
     6241 cttcatttca ttaaatgctg tctcattacc attcccacca actttagtta tcttatcaaa
     6301 actcttagag ttaactagag atttttgtga agctgataag ttctttccaa ctaaaactac
     6361 tggtgattga tttttagcac ctaatacacc tactgctagt gcatctataa gttggtcttg
     6421 tttattcata ccatctttag ttacataagc attttttaaa tctgtctttt tataaaattc
     6481 ttttattact tttgcatttg tatcatttct atctgttcca cctagtcttg tactatttgg
     6541 tagtttattt ttaacagctt cagaaattga actctctcca cctactacat aagattgagt
     6601 tattccagca tcttttataa atttgtctgc aactgctgtt ccatttttag aatcagcaag
     6661 tattactggc atattgtttt gtgcagctgg tgctcctaca cttacagcat ctgcaagtcc
     6721 tttttcacca ttaactacag ctattttctt aacatcactt atagcatcta atttctttgc
     6781 taattctaag gatgtttgat atctctcagc tccagagatt ctctcaaaag atatttttaa
     6841 attttttatt tctttttcta tatttgtact taatacagaa gttccaccta ttaagtatac
     6901 ttttttagct tttaatcttt gtatttctgc tttcgtctta tcatttaatt tatctttact
     6961 tgttaaaagt attggagcat tttttgcttt tgcaaatggt gttgcagata gagcatctgc
     7021 tatagatgag tcgtttacga ttactacctc atcagcattt ttccatccat cttgacttat
     7081 tttaacagca gtttcatatc tttcttgccc tgttaaattt gttgccacag tttgtgcact
     7141 agctatgcct gctgttgaac ttccagctat cattgtaact gtaagcattg cagcaatctt
     7201 ctttggtatt ttcattttat tttcttcccc cttgtccatt tgatattact taatttttta
     7261 actttgttcc cgttctaatt ctaaaggcta ctttttttat acaaaaccct attttttata
     7321 acaattcatt aacataaaaa tcaaacttac cttaaaatat aaatttacaa atttttcaat
     7381 caataagaaa atttctttat tccttacata atgactgcaa tgtaacaaaa taattacaaa
     7441 ttaattatga aatcattata gttagaaacc tctttaaaat tagatattaa gtatccttta
     7501 gaattaaatt acttactttg ctattttcca taaaatctat taatttactc ataaagtcat
     7561 atatattcaa aggttcaagc cctgtatgta taatctcacc ttttaatcac atacatatac
     7621 tttttcagtt ctatactcat attcttctga atccaagcag ttttcgccca atatatgatg
     7681 caaatcatat ataaatttgt gactatacaa ttcatctaag ttatctaatc catattctaa
     7741 ggctctatga ttattgtaaa tcatatattc acttttatta ctaggtttca tctttcctct
     7801 aattatagat tttgcaatct ttctagttga aaaagcacct tctattacac tagaataata
     7861 agactctagt gccaaatcat ttttatcttc tattgtttta aatatcttac ttgatgttaa
     7921 tttatctatt tcatttatta aatttaatat attttc
//
LOCUS       DAEMHW010000166         7937 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEMHW010000166 DAEMHW010000000
VERSION     DAEMHW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.166"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            140..1198
                     /locus_tag="KPK67_002998"
     CDS             140..1198
                     /locus_tag="KPK67_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBG2459930.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEDIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1302..2486
                     /locus_tag="KPK67_002999"
     CDS             1302..2486
                     /locus_tag="KPK67_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG2459931.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2693..3406
                     /gene="rpsB"
                     /locus_tag="KPK67_003000"
     CDS             2693..3406
                     /gene="rpsB"
                     /locus_tag="KPK67_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBG2459932.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3485..4396
                     /locus_tag="KPK67_003001"
     CDS             3485..4396
                     /locus_tag="KPK67_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBG2459933.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4481..5185
                     /locus_tag="KPK67_003002"
     CDS             4481..5185
                     /locus_tag="KPK67_003002"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBG2459934.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5211..5768
                     /gene="frr"
                     /locus_tag="KPK67_003003"
     CDS             5211..5768
                     /gene="frr"
                     /locus_tag="KPK67_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBG2459935.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5771..5974
                     /locus_tag="KPK67_003004"
     CDS             5771..5974
                     /locus_tag="KPK67_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459936.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            6003..6737
                     /locus_tag="KPK67_003005"
     CDS             6003..6737
                     /locus_tag="KPK67_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBG2459937.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6750..7529
                     /locus_tag="KPK67_003006"
     CDS             6750..7529
                     /locus_tag="KPK67_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBG2459938.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3279 a          800 c         1479 g         2379 t
ORIGIN      
        1 aaagcacaat gaagtagatt aaaaatttaa aactaagaat ctaatatata ttttgtaact
       61 atttaagtat ataaaataaa ataatatcca aactaataca aaatagtaat aattgttttg
      121 tactggagga tatatattta tgaattgtaa atggatatct aaaatagatg agagaaaaaa
      181 atgtcataga gaagcagatt cttctggata ttgtatcttt cataaagaaa ataagtctga
      241 tgaagatata cagttgatga tggatactct tcataaagaa gagataagtg aatttaatgg
      301 atttgttttt gaaaatgaat ttaatgcgga agaaatatta acttacaatt ataaaatact
      361 ggatttttct gaatccatat ttaaacaaaa agcaaatttt aaaaaatata tttttaaaaa
      421 gaatattatt tttaactata cagaatttag agataaagtt ttatttaatg gttgtgtatt
      481 cttagaaaac tgtgatttta atagaaccat atttagtaaa aactatataa atgaccgtat
      541 atttgaaaaa gtgaaattta aaggacctga tttggtagtt aataaagtag aaaattttcc
      601 acgaatggat ggaataattt ttagcatgtg tacaaaattt gtgttaaaga atgttgaata
      661 tggaaaaagt gagtatgagc atggcaagat aaattataga atagctagaa atcaagcaac
      721 aaaaattgga gaatatgaga tgataggatt ttactattat aaggaaagaa tttatagtag
      781 taaaataatg aaacgttcta attatcctac tttcagtgat tatttagttg aaaaattttt
      841 tgaccaaata gcacgttaca caacaggtta tggagaaaag ccatggaata tattattagt
      901 gattatagca ataataagtg tttttgcttt attatactta tttgtaggca tagaaagttc
      961 aaatagcaca cttgtagctt tagatataaa caatataggt gattactcac taagtgagat
     1021 tttcaaaatg tatatggatt tatggtattt tagcatggct acatttagta cagtaggata
     1081 tggagatatg gttgctacaa gtttgattgg aaaagcattg gcaggtatag aagtattttt
     1141 tggagttaca ataggagcaa tttgggcatc tgtgattatt aaaagaatga taaggtaata
     1201 tttgtgtaaa tatggatagg tattaattac tagatataaa ttggactaaa tagggaaaat
     1261 ttaaatataa atatacttaa taatccaaga aggtgaaaaa tttgaaaaat gcactaaaaa
     1321 aaattatatc attagccatg attctagttt ttataatacc aaattattca tatgcagatg
     1381 ataaatctgt agacaagtat tcaaaaacat ctatacttat agaccaagaa acaggtagag
     1441 ttttatatag taaagaacct gatatgaagg taccattagc tagtttaagt aagatgatga
     1501 cttttttact tgcaatagaa gctattgaaa ataaagaagt aaaagaaact gataaggtaa
     1561 aaatagacaa atctattgca tcagtaaaag ggtcaagtta taaacttaaa gatggtgaag
     1621 aagtgccact tatagagctt atgagaggtc ttatgattgt atcaggaaat gatgcggcaa
     1681 ttgctatagc aaaacatatt tgtaaaacga aagaagcatt tgtagataga atgaataaaa
     1741 aagcaaaaga aataggaatg agtaatacac attttctaaa tccaaatggt cttccaatat
     1801 atgatttaag caaccccaaa aaaccagcaa aagaaaatat ctctactgca aaagatatag
     1861 ctattcttgg aaaatacatg tttgaccatt atgaaaaaca agtaacagct ataactgata
     1921 tggagacata tacaaattca gatagaagtt ttgaaaaaag caatacaaat gcattattaa
     1981 gaattatacc agaagtagat ggtataaaaa ctggatatac tggcaatgct ggatattgct
     2041 tatcattttc tatggttgta aataaaaatg ataaaaatga gaaaaaacgt agagtaatag
     2101 gggttgtatt aggtactaac cataaaaata aaagaacctc tgcttcatta gctctactga
     2161 aatatggaaa agaaaatttc aatatgaaaa aagtaattga taaagatagc tttattggta
     2221 agaaatatat aaaaggaatg aaagagttag aggttacatt aaaagcaaaa gatgaacttt
     2281 atacaatatt aaaagatgat gaaagtataa aatcagaagt taagattaaa aatatagagt
     2341 accctgttaa aaaaggtgat actatgggta ttataaaata ttatactaat tcaggtgact
     2401 tacttggaag tgtagatata gtaagtaata atagtataga taacgtatca ttaaaaacaa
     2461 aattaaaaat gaaatttgca aattagattc aaaaaatggt ataaaataca caaaaaaata
     2521 taaaaataga aaatatggaa aaaatattgt tatttcttca cctctgtgat atactactag
     2581 aggtgaaaaa ataaaataaa tcacacacag cattgggatc cttaaaaggt gcctattaca
     2641 tggtttttta gggagaagat caagctggag gtataaaacc aggaggtaag atatgtcagt
     2701 aatatcaatg aaacaattat tagaagcagg tgttcacttt ggtcaccaaa caagaagatg
     2761 gaaccctaaa atggctaagt atatattcac agagagaaat ggaatatata taatcgactt
     2821 acaaaaaaca gttaaaaaag ttgaagaagc ttacaagttc actaaagaag tagctgaaac
     2881 aggaaagcca atcttattcg taggaactaa aaaacaagct caagatgcga taaaagatga
     2941 agctgaaaga tgtggaatgt actttgttaa tgaaagatgg ttaggtggaa tgttaacaaa
     3001 ccataaaact ataaaaacaa gaataaataa attaagagaa ttagaaaaaa tggaagaaga
     3061 aggcgtattt aatgttcttc ctaaaaaaga agttataaaa ttaagagctg aaaaagaaaa
     3121 actagaaaaa tacttaggtg gaataaaaga catgcctgaa ttaccaggtg caatgttcgt
     3181 agttgaccca agaaaagaaa acatagctat tcaagaagct catagattag gtataccagt
     3241 agttggtata gttgatacta actgtgaccc agaacaatta gactttgcta taccaggaaa
     3301 tgatgacgct ataagagccg taaaattaat aactggtgct atggcaacag ctgtaattga
     3361 aggtagacaa ggtgctgagg aagaagtagc tgaagaccaa gaataataat ttgacaatta
     3421 aatggtaagg gtcgccctta ccatttataa tatggaatag aataagtagg aggaaactta
     3481 aaaaatggct aatataactg ctcaaatggt aaaagagtta agagaaagta caggcgcagg
     3541 aatgatggac tgcaagaaag ctttacaaga agctgaagga aacatggaaa aagcagtaga
     3601 tttattaaga gaaaaaggat tatcaaaagc tgctaaaaaa gctggtagag ttgctgctga
     3661 aggtctagtt gcaatagaaa tgaatgatga taatacagtt gcttctatgg tagaagttaa
     3721 ctcagagaca gatttcgttg ctaaaaacga agacttcaaa gtgttcgtta aagatgctgc
     3781 ttgtatggca ttagctactg ataaagaaga tatagcttct ttattaggtg aaactcataa
     3841 agagggaata actttacaag aagttttaaa taatagagtt gcaaaaatag gtgagaaatt
     3901 agactttaga agatttgcta aagttgtaac taatggacaa gttgctggat acattcacgg
     3961 tggtgggaaa ataggtgttc ttgttgaaat ggaaacagaa gctagagatg ctaaagtttt
     4021 agaattaggt aaagatgtag ctatgcaagt tgctgctatg aatcctaagt atgtttcaag
     4081 agatgaagta gatgctgaat acatagcaca tgaaacagaa gtattaactc aacaagcttt
     4141 aaatgaagga aaaccagcta atatagttga aaaaatggtt aaaggaagat tagaaaaaga
     4201 attaaaagag gtttgtttat tagagcaaac tttcgttaaa aatccagata taactgttaa
     4261 acaattagtt gctgatgttg ctaaggcagt aggttctgat ataaaagttg ttaaagttgt
     4321 gagattcgaa gttggtgaag gtatacaaaa gagagaagaa aactttgcag aagaagttgc
     4381 taagcaactt aagtaataac caaagagaga acacgcaggt gttctctttt ttaaaaaata
     4441 gaaaaaattg gatatgaagg attgaaatag gaggtttctg atggataaac caatgtacaa
     4501 aagggtattg ttaaaattaa gtggagaagc attagcagga gaaagaggtt ttggtataaa
     4561 taatgatgta gttaatgata ttgctattgc tataaaaaaa atacaagaaa taggtgttga
     4621 agtagctgta gtagtaggtg gaggaaactt ctggagaggc agaaccagtg aaggaatgga
     4681 cagaactact gctgactata taggaatgtt agctacagtt atgaatgcaa tggcgctaca
     4741 ggatgcacta gaaaatatag atgttgctac aagagttcaa actgctatag acatgagaca
     4801 aattgcagaa ccatatataa gaagaagagc agttaggcac ttagaaaagg aaagagttgt
     4861 tatatttggt gcaggaacag gaaaccctta ctttacaaca gatacaactg ctgctttacg
     4921 tgctgctgag atggaagcag aagttatatt gttagctaaa aatgttgatg cagtatatga
     4981 caaggatcca aaagtacatg cagatgctaa gaaatttaca gaattaagtt acatggaagt
     5041 tatacagaaa gaattaaaag taatggattc tacagctaca tctttatgta tggataataa
     5101 aattccaata aaggtatttg agcttacaac agagaatata ataagagcag taaaaggcga
     5161 aaatatagga actactgtaa aataatgaag aatgaaggag gagtaaaatt atgaaactgg
     5221 aaatacataa gcaattagaa gagaaaatga atgggacaat tgatgcttta aagtttgaat
     5281 ttggaactat aagagcagga agagctaatg cacaaatgct agacaaaata agagttgatt
     5341 attatggaac tccaactcca ataaatcaaa taggtgctat atctgtacca gaaccaagaa
     5401 tattaatgat atctccttgg gataaatctg ctatgcatga aatagaaaaa gcgatagcta
     5461 actcagactt aggattaaat ccatcaaatg atggtgaggt tataagactt tctgtacctg
     5521 ctttaacaga agaaagaaga aaagaattag ctaaaaaagc tagtaaagct gcagaagaat
     5581 ttaaagttag aataagaaat gaaagaagag acgccaatga gaaaattaaa aagatggaaa
     5641 aaggcggaga attaactgaa gatgaattaa aaaaagcaca agatgaagtt caaaaaatga
     5701 ctgataaatt tataaaagaa atagatacat tattatctaa aaaagaaaag gacataatgg
     5761 aggtataata ttgaaaaaat attataacct tttaggtctt catgtagatg aggtaaaaca
     5821 atattttgat gagcaaaata taaattatac cgtaaaatct attgaaggta aaaaggataa
     5881 agaaaaactt gtagttccta gagttataaa aatatctgag ataggtgata gtgtagaact
     5941 gataatcaca tacttttctg actccttagt ttaaggagtc agaacaatat tggaggcaaa
     6001 atatgaataa caacattata tatgatatag acttaaataa catacctact catattgcta
     6061 ttataatgga tggaaatgga agatgggcaa aagctagatt tcttccaaga actgcaggac
     6121 ataaggctgg agtagaaact ataagagata tagtaaaaga atgctcaaaa ttaggtgtta
     6181 agcacttaac attatatgca ttttcaacag aaaactggaa aagaccgaaa cttgaagttg
     6241 acacacttat gaacttatta tctacatact taagaaatga aatagctgaa cttcaccaaa
     6301 acaatgtaaa agtaactgct ataggagata taagtgcttt accaaaaact tgtataagag
     6361 aattaaactc agctaaggaa ctgacaaaaa acaatacagg agttaactta aatttagcat
     6421 taaactacgg aagtagagca gatataaaaa atgctcttat agatatagta aaaaattgtg
     6481 aaagtggtaa aatagatata aacaatattg atgaagatat tataaaaaac tatttaagca
     6541 caaagtctat accagatcca gacttggtaa taagaacaag tggtgaacaa agattgagta
     6601 actttttact ttgggaagtt gcttattctg agttttactt tactgatata cattggcctg
     6661 attttaaaaa agaagaacta cagaaggcta tatacgtata tcaaagaagg gatagaagat
     6721 ttggaggact taagtaagga gattatttta tgcttacaag aattattgct tcactagctc
     6781 tggttccatt atttctattt gttgtatatg gaggtattcc tctatatata gctgagacag
     6841 caatagttta tattgcattg catgagtttt ataaggcatt taaaataaaa gatgtacatc
     6901 caatattcat tataggatat ttattttcta tttatttagc tgtaaaaaat atttttaatt
     6961 taccattaga atatacttat gcagtaattt ttattttatt tttagcaagt ataatttata
     7021 tgttgatggg taaaaacaat gttatagatg tttcgattac gtttttagga gttttttata
     7081 taggagtatt tttagatttt ataattataa ctataaatgg atttgaaaaa ggtagtatat
     7141 atgtatggct tatatttgtg atatctttta tgacagatat atttgcatat tttagtggtt
     7201 atttacttgg aaaacataag ctaattccta aagttagtcc taaaaagact atagaaggag
     7261 ctattggagg aataatagga agtacacttt gttgtatttt atttggatat ttatttggca
     7321 ttgatttgct tcaattagcg ataataggaa gtataggaag tgtaatagct caattaggag
     7381 atttatttgc atcttctata aagagatatg tagggataaa ggattatgga aagataatac
     7441 ctggtcatgg gggtatatta gatagatttg atagtgttat attagttgca ccttttgtat
     7501 atagtgcgat taaatttttt attagataaa gattggttgg atttaaaaga gcaatctata
     7561 attgctagta atgaaaatag ctatgtaact tttaaatgca gagatattct gcagttatta
     7621 gttacatggc tattttcatt gttcaaaaat ttagaagacc ttggggaatt gtataagaat
     7681 aggtattact ttataatagt atactatatt tgtagtgagt atatttttta tactgtaact
     7741 atcattaaaa aatatttttt tgtttaataa tttcgatatt tttagcttta ttatatattt
     7801 ataatatata tgtataaatg taactaattt attatccatt tataaatttt atttttaaat
     7861 ataattaata agagataagt aaattattgg tcttattgca caaaaataga tataatttaa
     7921 gtaatatacc aaaaaaa
//
LOCUS       DAEMHW010000167         7841 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEMHW010000167 DAEMHW010000000
VERSION     DAEMHW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.167"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..136)
                     /locus_tag="KPK67_003007"
     CDS             complement(<1..136)
                     /locus_tag="KPK67_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG2459939.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     L"
     gene            complement(503..1933)
                     /locus_tag="KPK67_003008"
     CDS             complement(503..1933)
                     /locus_tag="KPK67_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG2459940.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEEICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            2277..2942
                     /locus_tag="KPK67_003009"
     CDS             2277..2942
                     /locus_tag="KPK67_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459941.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            complement(3431..6133)
                     /locus_tag="KPK67_003010"
     CDS             complement(3431..6133)
                     /locus_tag="KPK67_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2459942.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKAQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSVQQIVHKQCANDILSCIENCGLDKKYISVEVTETVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQIAKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(6159..7709)
                     /locus_tag="KPK67_003011"
     CDS             complement(6159..7709)
                     /locus_tag="KPK67_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2459943.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDSCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
BASE COUNT         2683 a         1283 c          740 g         3135 t
ORIGIN      
        1 acagcatggt ggttatatta actatgaaag ctattaatat ttcaaatcca taagaataga
       61 ctttagattc attattatct atcatattat tacaaattaa aacagatgtc attttttcag
      121 catatctttt aaacattttt acctccaaaa aaattttaca tatatagagt acattctttt
      181 ttgagaaaat tcaatatact ttacaaaact agttgttaaa aatgtaaaac tggtcaaaaa
      241 aagacctata taattttctt cttattattt atataacaaa acaattataa taaaacaatt
      301 ttatcaagct ttatatgcat aaaaagctat gtcaaaatag aaataaacct tttccccatt
      361 gttaaaaagc taaaaatata ttttatttca tataaaaaga gtatctcaca aactaaaaag
      421 ttcattttga gatacccatt ttatttatta caaaatatca aattttattt atttgcatat
      481 attttctata tattttagcc ttttaacttt gtttttgttt tacatactgt agtgcttttc
      541 tattctgttt tactgcttca agacaaactt tttctgtttg attatcaaca aattctaaag
      601 ctctataatt ttgctttact gcttctaaac atattttttc tgtttgatta tttacatatt
      661 tcaacattct ataatcttgt tttactgctt ttagacaaac ttcttctgtt tgttctttca
      721 catatttaag cgcttcataa ctttgattaa ctgcttctaa acaaatctct tctgtttgtt
      781 cctttacata agctaaagca ttatagtttt ctcttactgc ctctaaacat attaattctg
      841 tctgattttc tacatattta agaatttttc cattttgctt tactgcttct acaaatattc
      901 tttctgttgg tatctttaca taacttaata tttcttttat ttcaagttct tcaaatgaac
      961 ctcttactgc ttctatacat atattttcag tctgtttttt tacatattgc agtacttttc
     1021 cattttgtct aactgactcc aagcaaattt cttctgtttg ctcttttacg tactgtaatg
     1081 ctcttccatc aattcttact gcttccaaac aaatctcttc tgtttgctta tttacatact
     1141 gtagagccat ataacttctt tttactgctt ccatacatat tttttctgtc tgttcattta
     1201 cataatacaa tgcaaagtca ttctgtttta ctgcttctat acatatttct tcagtttgct
     1261 cttttacaca agatagtgca ctgtagtttt cttttacagc ttctaaacat atactattac
     1321 tttgttcctt tacataagct aaagattttc catctttttt tacagcttct aaacatatgg
     1381 ctggagtttg ctcttttatt ccttttaata tctctgcaac atcattttta gatgcccttt
     1441 ctataaatgc tagacataca tcttctgttt gctcttccac aaatttaaat acttccatga
     1501 catcataagt tgatgcattt tttatagcct caattagtac attttctgtc ttattattta
     1561 tgtagtacaa agctctgtag ttctgtttta ctgcttctat acaaatatcc tcattttgat
     1621 ttgttatata cttaagagat ttatatgtct gttttactgc ttccatacat actttataag
     1681 tcttgtcctt tacaaactca attatagtac catcctgctt tactgctagc agacacatct
     1741 cttctgtttg gttttctatg tcttttaact ctctgtaatc atattttact ctgcttaaat
     1801 catttaaatg tactttgtta tcttccttta aatcttcttc attattttct atatcatact
     1861 caaattcttc atcatctaaa tagtcatttt tttctggtaa aaatatagat ttaattttat
     1921 tctttatatc cataaaatta tcctccttta atatgattct atcattaaag taaatcgttt
     1981 ttaagcactt atttaattaa ttttatatta agtttattta aaagtctatt cattgtcgat
     2041 agattatatt ttatattaat aattttatct tatatatgtg gggtaataaa gttaatagtg
     2101 gacattttcc atcccattag tgtacactat ttatatttat tactcattta tagtgatttt
     2161 tttcaaattt tttcttattt actatatcat atttattcaa tttatgatat tatttaaata
     2221 taatataagt tttataaagt atattaaata tattataaga tggaggtcga gtaattttga
     2281 aaggaaacat agtacaatat aattttgcag atatagagga agaagtatat tcgttggatt
     2341 atgctatagc atggaataca gacgaggaaa acgtaaacat aataccattt acaaataaat
     2401 tctgtaaaga atctatagaa tctttttgcc taggtaaaat taataacttt gttgaaatat
     2461 taaatgaagg ttttgttgaa aatcatcatt atgtacattt agataaaatg attagtgttc
     2521 ctaagaaaaa ggttaattta gtatatcaac aagacacaca tggttatctt ctaagagatg
     2581 ataatgacaa ccttattcct gcaaaaataa cttcagagca atctaaatct atatcaagta
     2641 agatggagtt attctgtgct ggtgaagaaa aatgtttaat taatatatta ctaaaggctg
     2701 acccatcata tattttagat gtagatagta taaaagacaa aaacattcta aatctaggtt
     2761 atgagtctat tgacagatat aaagaataca attttgatga tgataagatt ttaatatttt
     2821 ttataaataa aaaacgttat tcagtgatta tgaaaaaaac aaacaactct gataacgatt
     2881 tagtttccag aaacaatgct attaaagaat tatttactaa taaagcagga aatcttaact
     2941 agtattataa ttttaaatta ataatagtat acaaagaggc tgtctcaaaa tagagataag
     3001 cttctttttc attgttgaaa taataaaata tatttaattc tatataaaag agagtgcctc
     3061 ataaactaaa aagttcattt tgagacaccc tcctatttat aataaacaaa taaatcctta
     3121 aacctatcga gtgattataa tttttagatt gggttatctt gcatttttaa atttaaggta
     3181 actttctact ctaaacttta atctctaatc aatatcatca actgtaacaa cttacaatta
     3241 aatctttaat aatcaaactt tagactatct atattaaaac tcaaattaga tttaggattt
     3301 atttggatat cacatttatt ttattaacta ctaatcaatt atttttactt ataaacttat
     3361 tcttccctaa ttctattatt aaatactttt aattattttt caatacctta aatctttatt
     3421 ttattaacct ttattcttca atcataagtt caataaattt atcttcactt aaaggttctg
     3481 aaaaataatg accttgaatc aaattcacac ctgaagttac aactaaatcc agagtttctt
     3541 ttgtttcaac accttctgct acaatatcca tgccattaat cttagcaatt tgaactatag
     3601 tttttaataa ttcaaattgt tttttagaat aggtaatttg ttctactaat tccttatcta
     3661 tttttaaatt ttcaataggt aaatttgcca gataatttat actactaaat ccagaaccaa
     3721 actcatctaa agctatgcta atgccttctt tttttagttt atcaagagta acatttacaa
     3781 tttcaaaagt ttccataagt actgtttctg taacttcaac agaaatatac tttttatcta
     3841 aaccacaatt ctcaatacat gataatatgt catttgcaca ctgcttatgt acaatctgct
     3901 gaacagataa atttaattgc atttgttcaa aatctatatt tttatctttt atttttgtag
     3961 ttacatcaca agcctttttt agtacatata gacctacatc cttaataagt ccatgctttt
     4021 ctgcataagt aataacttca taagcagaat aataaccgcc ctctttattt ctcaatctaa
     4081 ttagtgcctc agcttttata taacagtttt tactaacatt aaaaattggc tgataccaaa
     4141 cttctagtgt ttgctcttcc agaccttttt ttaaatcctt aataatatta ttttcatggt
     4201 aaatctcttc tgtcaattcc ttatcataaa ctttaacatt acttttgaca tccttttttg
     4261 catattccaa tgcaatttgt gccttttcca gaaggtcata tggattttca ccatgatatg
     4321 gatattgaca aataccaata ttacatttta catcaaaaag tacatcttct acacttaaag
     4381 aactattaaa tacttcatta accttatgta aagtatctaa gtaagattct ttatctattg
     4441 aaatgcctaa aaatacatct ccattaatac gatatagata attagaaaaa attgttttta
     4501 atttgttact aacttctttt aaaataacat ctcctatttc aatagaaaac agttcattaa
     4561 attttccaaa cttgcatatg tctattaaaa ataagtataa cattctctca gaattataat
     4621 caattaatac ttcgacatca cgcatatacc ctcgtctatt tccaatatca atattataat
     4681 ctttatacat aatacgctgt acttttttat aatatttgta tattacttta gacaacttgt
     4741 ttatttctaa atctggatat tcaccaaata caatctgttt cttttcatca agcatatctt
     4801 cacacttttt agtaaaatca tgaataggag acattgctgg aaatcttctc tcataatctt
     4861 caatagctga aagtaacaga gattctgcat cttttctatc atcaacacac ctagatgagc
     4921 cataaccaat cgttaaaaat acttgtttat tttctacttt aatttttcgt aaaagctcta
     4981 aggaaatccc tttttcaatg ttaagcaatt catttttatc acaattttct tctaaagcta
     5041 gaacaaaacc ttcctcactt atacgagcaa taaaatgaga actttttatg taattatcta
     5101 gattttttac tatatgtgta agataatctt gtcttttttc aaagctcata actggagcaa
     5161 atttactata attagtaata catatataca gcaacactat ttcattattt tttctattca
     5221 gatgctcttg taacttaatt aaaaaacctt ctctattcaa aataccagtt tctaaatttt
     5281 taagactctg ttgatttaat tccttactaa tcgacttctt ttcaatgtaa ataaatgtca
     5341 tcacacctat taatatggaa ataattaaca atataacaaa tacaataaaa tctatattct
     5401 tattattcca tccatttttg tacacaatgc taagagtcca aacagtcgga agtttaaact
     5461 taatttcatg tgatttggaa aaattaaaat ctttagtaga aatttctatt gtagattttg
     5521 aaccattatc taggtgtaca cgccaaagtt catactcaaa tccagattct tttaatttat
     5581 ttaagttaaa agaatctaca acttttttgg catctaaagc tactgtaatt tctccccagt
     5641 actctccatt tttataaata ggattagtaa ttaaaaacac atctccattt gaaaactggt
     5701 ctttagaagg accttctacg actaatcctt tgatagaatt tgctaatgaa taagaataat
     5761 tcagttcctg tattggagta ttgataatat cttttttgac agaattagaa aaaaatgcag
     5821 ctacattata tttttctaca actgaaatat atatgcaaga attgttttta agaatttcat
     5881 ctgcaatagg ttttaattta tctatagaat aattgtttga aactaaaact gatgtcaagt
     5941 ctttgctata attaaatttt tcagtcagat ttaaaataat gttattcttg tagacattta
     6001 acatgttttc tactactttt gtttctttct tttcttgttt gtcaaaaaca taaatagcaa
     6061 atttactaaa acataaaaat gttattaata caataatagt acatacaaaa aattttttag
     6121 atacattttt catatgatat aaactccttc cacatacatt aaactaaatg ataattacct
     6181 tttccattac gtttaacata gtataaagct tcatcagcct gttttaataa attatctata
     6241 tctttattgt cttttacact acatactaca gctccaacac tacaatgagt ttgaatgata
     6301 ccatcatctg ttagtacttc agtatgtaag cgaatagcaa tctcatccaa taaatcctcc
     6361 acctcttctc tcgatgtaaa accagataac aacataacaa attcatctcc accatagcgc
     6421 ccaattaaac cattatattt tatctcatat tcacgaagta catatccaac atttttcaaa
     6481 accatatccc caatttcatg tccatataag tcgttaatac atttcaaatc atctaaatca
     6541 acaaatgaaa ttacagataa tttatcattt tcctccattt caacacgatt ataaatttgg
     6601 tcaaaaaata cagtacgtgt tacaacacca gttaatccat cagtcagacc tttacttgtg
     6661 agctgaataa tttctttaaa gtctacttca ctaggtcttt ctgtttgata tatacgtttt
     6721 tctaattctt gaacagaacc taaaagcata tcaataactt ccattcgttt tttcatgaag
     6781 cttttctcca tacaaatcaa aataataaat actaaagtta taacagatag aatagctccc
     6841 ataggaatta aatttaatgc aaaaatatct aagaaattaa ctttacaaaa taaactccaa
     6901 tttgtgttct taattggttt ataggacata aagtataatt ttccattttc aaaagcccaa
     6961 taatcacctg atttattatt aaacatatcc ttttctactt tttttgcatt agttccaaga
     7021 tattttgttt tctgcgataa cttcatatac ttctctccat atgcagtttc atcacttgtt
     7081 aaattcatta tttgattttc acttccaaat aatatgaaat tctgttgctg ttgacctttt
     7141 ccttgtttga gtatatcctt tatctcatca aagtaaattg ctgcaaatac acaactatct
     7201 acatttccat tctttattat aggtgatgca atagtgtagt ttaaagtttt tccatcagcc
     7261 cctgctgcaa aactatctgt aacttgatat ttttttgttg caaaaagttt ctgcatataa
     7321 tcacgaccag ctagactaat attaccacct tcttcatccc atacaattat attcttatca
     7381 acaaaacata tcatcatata cccaaattca tcactataca ttgaaagttc tttactttta
     7441 tctatgccat tcacactatt atctattatg taattatctt tactcaaaga gttcaatagc
     7501 ttaattgttt ctcctaccct atcttcaata cgatttgacg ttgtagataa taaatcttga
     7561 ttatcttttt tcataaccca tattgtattt atacaaaaca tagaaagaat aattaatata
     7621 attactatat agaaagttat aaaatatttt gctagagatt gaaatgaaag ttcacttgac
     7681 ttatctatat ttcttttttt gtacctcata attcctaccc ccactataga aaaattgatg
     7741 aaaatgttcc tattagaacc ttaataatac attacaaaac acataatttc aagtaaaacg
     7801 ttaaattttc attactaaat ataaactttt tattacaata t
//
LOCUS       DAEMHW010000168         7805 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEMHW010000168 DAEMHW010000000
VERSION     DAEMHW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.168"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(160..939)
                     /locus_tag="KPK67_003012"
     CDS             complement(160..939)
                     /locus_tag="KPK67_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG2459944.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYNGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(996..2252)
                     /locus_tag="KPK67_003013"
     CDS             complement(996..2252)
                     /locus_tag="KPK67_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBG2459945.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEILESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDTVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTEEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            2510..3409
                     /locus_tag="KPK67_003014"
     CDS             2510..3409
                     /locus_tag="KPK67_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBG2459946.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            3433..4881
                     /gene="ddlR"
                     /locus_tag="KPK67_003015"
     CDS             3433..4881
                     /gene="ddlR"
                     /locus_tag="KPK67_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBG2459947.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            4940..5746
                     /locus_tag="KPK67_003016"
     CDS             4940..5746
                     /locus_tag="KPK67_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2459948.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            5772..7691
                     /locus_tag="KPK67_003017"
     CDS             5772..7691
                     /locus_tag="KPK67_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2459949.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDYDIEKLESKIATLDESTAKFSTDFTKLQKILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
BASE COUNT         2948 a         1080 c         1119 g         2658 t
ORIGIN      
        1 ttgactctat tttatctaat acaataaaat catatcatat agcaaattgc ggttaactta
       61 agaattttta aacttatata aacttataat tgaatataat taaaatgact gctctataga
      121 atataatttt atacttctct aggacagtca ttttttatac taactattta tctccattat
      181 aacatctatg gcacttttta cactatctac tacataattt gcactttgct ttacatcttc
      241 ccttgcctta gacattgcaa aactatattt aaatccttct aacatgcaaa tatcatttgg
      301 agaatcccca atagtagcaa cttcatctgg acttacattt aacgttttta ttacatgctc
      361 tatacttcct ctttttgaca cacttttagg aactatattt aaagtatatg ttccagaaaa
      421 atatatttct aatacttcac taaactcact ttttagataa agttctattc tggataactc
      481 ttccaaattt tcagaacata cgcctatatt tcctataaga ttatcaaaac tacttctttt
      541 ttcaaaatct gggtcaacta taaagtcact agaataatgt ctaaaagcag tggtatcact
      601 acccacaatt acatcaccat tatatttaaa ataaagttgt gcatcacttg actcaaatct
      661 atcaatgact ttttttacta aattatcgtc tatcgttctt ttaaaaatta cattataatc
      721 tttatcatat ataaatgaac cattttcaca tacataatat ccattcatat ctaaactatc
      781 tttgataata ttatgtgctt gtttaaaaat tctacctgta gcaacattaa actgtactcc
      841 ctttttttct atttcttcaa ttgctactat atcttctttt gttatggttc cattttcata
      901 tagagttcca tctaaatcac aaaatatatg ttttatcata aattaaaagc ctccaaaaca
      961 attttatatt atactaatac tttaatcaaa ataacttaaa gcttttctaa ttgtattctc
     1021 aattcattaa gtaaatctct agtagctctt aatcttatct ttcttctatc cccattaaat
     1081 atatttttct ttactattgt ttttccattt atatatattg cagtataaac tagtcccaca
     1141 ggcttctctt cggttcctcc accaggtcca gctactccag ttgtagatat acctatatta
     1201 gctttaagtc ctttagcaac gccctcagcc atttctatag ctgtttgctc acttactgct
     1261 ccaaactctt ccaaagtttc tctttttact cctaatcttt tcattttaga atcattagaa
     1321 tatgttacac aaccttcaag aaatacagag gatatacctg gatagtttat aagagatgaa
     1381 gaaaccaaac ctccagtaca agattcagcc actgctattg ttagattttt ttctacaagt
     1441 agcttagaaa cagtatcttc taaagcagtc tccccttcag atatggaaat atctccttca
     1501 gtatatacat atttaccaac tctacttttt attttttctt ctactggttt tattaattta
     1561 aaagcttctc tttcattttc agcctttgca gttattctta ttgtaacttc taattctttt
     1621 gcatataaag caactgttgg attagtctgc tcattaatta tatcgagaat ttctaattct
     1681 agatttgact cacctattcc atataatctt aaagtcttag atactagcat ttcatttgtg
     1741 aattgttgta agtaaggttt gacactctca ttgaacatag ctttcatttc tcttggaggt
     1801 ccaggcaata ctattataaa tttaccatct ttttttaaaa ttgcccctgg agctgtacca
     1861 ttattatttt ttagtactat agcatctttt ggaaagtaca cttgtttcat attattttcg
     1921 gccatttttt tccctgtttt aacaaagaaa gattccaaaa tctctagtga aggtttatgt
     1981 agttctaatt tctgaccaaa gtattctgca gcagtttctt ttgtcatatc atcaccagtt
     2041 ggaccaagac cacctgtagt aattacaaaa tcacttctct ttaagctttc atcaaaacat
     2101 tctaaaagtc tttgtttgtt atctccaaca gtactctgat ggtatacttc tattcccaat
     2161 gatgcaagtt ctttagctaa aaattgagaa tttgtattta ctatatctcc caataatatt
     2221 tctgttccta cacttattat ttcagctttc aaaatttatc actccttgat ttatagccat
     2281 taagactagt cttaagttca taacaataat tttagcaaaa ataataacat aaataaagtt
     2341 aaaaaatcat ttaaacatat ctttaaacta gattattatt ctaaaaccac acataatata
     2401 aacttcgtat atagtggtac tttcatcctc aactgttaat tcatataatt aagtatgtaa
     2461 ataaaaagtt tactttaaaa taataaaaat taaaatagga gtgtataata tgaaaatagc
     2521 agttataatg ggtggaatat catcagagag agaagtttct ttaaattcag gaaaagagat
     2581 atataataac ctcgataaaa ataaatatga agttgtaaaa gtaataatag atgataaaaa
     2641 agatatattt acaaagatac cagaagatat agattttgca atacttgctt tacatggaaa
     2701 atttggtgaa gatggatgta tacaatcaat acttgagact atggatatac catactctgg
     2761 atgtggacct ctttgcagtg ggatgtgtat ggataaaaat ataactaaaa aaatgcttag
     2821 agattcaaac ctacctactg ctccatgggt tttagtaaaa tcagttgatg aaatagacta
     2881 tgatgaaata gataatatag gttatccagt atttataaaa ccaaactcag gaggttcaag
     2941 tgtagccaca ttctttattc attctaagga tgaagttgaa gaagcagtta gaaaaggtct
     3001 ggaagttgat gaatttgtaa tgattgaaaa atatatacca ggtggagaat atacttcatt
     3061 catattaaat ggagaagtgt tccctacaat atctataaaa tctgattcag gattctttga
     3121 ttatgaagct aaatactcag ttgaaaaagg tgcaaaggaa gaagttgttt acttagatga
     3181 agaacttcaa aaaagagtaa acgaaatttc tgagacttgt tggaaaattt ttaattgcaa
     3241 agcatatgta agagttgata tgattataag tgaaggtatt ccatatgttc ttgaactaaa
     3301 tactcttcct ggaatgactc aaactagttt aattcctaga agtgctgctg ctagaggtat
     3361 taagtacagt gaactattag ataaacttat agaatactct ttaaattaga tgaaataatt
     3421 tgaggtggta atttgatatt ttcaaatcta aaattaaatg ataatgaacc aatttatata
     3481 caacttaaaa attatataag tgatatgata tcaaaaggac taattccaga caatagtaag
     3541 ctcccttcta caagggagct tagtcagctt ttacaagtta gccgaaattc tgtagtttta
     3601 acttatgaag aattaaaatc agaaggatta atctactcta tttcaggaaa aggtacattt
     3661 gttaaatcaa agaataagtc ttcaaatact acatggtcat taaactggga ttgtttagag
     3721 aatacatact ctaaaaaagc caatgaattg gacattataa aaagtgaaat tccttggagc
     3781 tctgacctga tttcctttaa aagtatttct ccagatggag atttattcga catggaagaa
     3841 ttaaaaaaat cctttttaaa tagaatttca ctagaaggac ataaattact caactatgga
     3901 tatgcacaag gttataagcc acttattgac tatttactag aatatatgac aaacaaaggt
     3961 gctgatataa gtaataaaga tatactaata acaaatggat tcacagaagg ctttgatatt
     4021 attatgtcat cttttaccca agaaaaagac tacataatat gtgaaaatcc tactcataat
     4081 actgctataa aaattatgaa atctcataac ctcaatatct taggtgtaga tataaatgaa
     4141 gatggtttgg acttcaatat gctagaagac aatttagtta agtataaaaa taaaataaag
     4201 tttgcataca ttacaccctc ttatcacaat cctacaggaa ttgtaatgac tcctgaaaat
     4261 aggtacaaat tctataatct tatgaaaaaa tacaatatag caattataga agatggattt
     4321 aatgaagaat tactctacaa tagttctcat atatttccta tctgttccct agataatatg
     4381 aataatgggg tcgtatatat tggtagtttt tcaaaaattt tatttcctgg aatgagaatt
     4441 ggatggatat ttgcagataa aaaagtaatc aataagctag aaagtgtaaa aaggtgcaga
     4501 aacatacatg tatccttttt agaccaagga attttatttg attatttaag taatggaggt
     4561 tttgaaaaat acattaaaaa aatacgtaaa ttctatggag acaaatttaa ctttgcatat
     4621 aagtgtataa aaaaatatat aaaaacagaa tatatcttgg gtgatggtgg tttgcatata
     4681 tttataaaac taaaaaatat agatactaga gttttattag aaaaatgtta tgaaaaaaat
     4741 gttatattta tgcctggaga tttatttttt acagataact ctggttttga tactttaaga
     4801 ctcggatttt ctaggttgtc ttttgaagac attgaaattg gaataaaaat aattggagaa
     4861 actataaatg aaatgttcta ggttaactaa atattgttag cctagtttat ttttatgagg
     4921 tataatatat atagtttata tgaatctaat actgggggat aatatgagta aaaagtcaaa
     4981 acgaagaaaa attaataaat taaaattaac gattgtaata actgtattat ttcttgcttt
     5041 attatctata tatgagatac tgttctctaa tcccaacaaa aaaggtaata taaagagtca
     5101 agaaactatg caaaataaaa ataatacaac agatgattct aaaaataatc aaagctctac
     5161 caaaaagcag gaagataata atatcaagcc tactttttca tattcttcaa taccagataa
     5221 tataaaaaat aaaatgattg gtaaatctat gcctactgat gaacctataa gctttgatag
     5281 tctatcttat ttaaaactta catattatgg atttgatgaa aaaactcatc aaggagaaat
     5341 gatagtaaac agtgaactag ctccagaagt agtggatatt tttaaggaat tatacgaaaa
     5401 aaaatatcca attgaaaaaa ttaagttaat agatgactat gatgctgtag atgaaaaatc
     5461 tatgtctgat aataatacct catcattttg ttatagaact atagctggta caaatgtagt
     5521 ttcaaatcat ggtaaaggac gtgctataga tataaaccca ttacagaatc ctcaagtttc
     5581 tggaaatgat gttacaccta aagtaagtac agtttatgct gataggtcta gcacaaaatt
     5641 tggaatgata aaaaaaggtg atgattgtta taatgctttt gtaagcagag gttggagttg
     5701 gggtggctat tggaaaaacc cagattatca acattttgaa aaataataga ttagaaataa
     5761 aggtgaatta tatgaattta atgacattgg aaaatataag taaaagctat tctgaaaaaa
     5821 aattactaga aaatatatct cttggaataa atgaagggga aaaaatagga cttattggtg
     5881 taaatggtac tggtaaatct accttactca aaattattgc aggagttgaa gaaagtgaga
     5941 ctggtaatat aacaaaaact aatggtatta ggatagaata tctacctcaa aatccaactt
     6001 acaatgaaaa ttcaactgta cttgaacaag tatttaatgg aacttctgaa gaattaaagc
     6061 tacttggtga atatcaagaa atcctagaaa aacttaattc aaactttaca gaggaattaa
     6121 ataaaaaatt attatcagta cacgaaaaaa tagatgcctt aaatctttgg gatttagaaa
     6181 gtgaagctaa gtcagtactt acaaagcttg gtataaatga ctttaatcaa aaaatagcag
     6241 aactttctgg tgggcaaaga aagagagtct ctctggcatc agcccttatt acaccttgtg
     6301 agtttttgat actagatgag cctacaaacc atctagacaa tgatactata gattggcttg
     6361 aggaatatct taattccaga aaaggctcat tgcttatgat aactcatgat agatactttt
     6421 tagaccgtgt tacaaataga attattgaac tggacaaagg tagattattc agttatgatg
     6481 ggaattattc aatatttttg gaaaagaaaa tggaaagact ttcgctagaa gcaagtattg
     6541 aagataaaag acaaaattta ataagaacag agcttgcgtg ggtaagacga ggagctcagg
     6601 ctcgtagcac taaacaaaaa gctcgtttac aaagatttga tgaacttata aatagagatt
     6661 cttttaaacc tgatgaaaat ctcgatatat ctgttgcatc aagtagactt ggaaataaaa
     6721 taatagaaat acacaatata tctaaatcat ttgaagaaaa tactgttata gataatttag
     6781 aatacacttt aactcgtaca gatagaattg gtataatagg caaaaatggt atgggtaaat
     6841 ctacccttat aaatatactt aatggagaaa tagagcctga cagtggatat atttcaattg
     6901 gagaaacagt taaaataggt tgtttttctc aagacgactc tcatatgaat attgatatga
     6961 gggctataga ctatgtaaaa gaagctagtg actatataga aacttcagat ggtactagga
     7021 ttacagcttc tcaaatgtgt gaaagattcc tttttaatgg tactatgcaa tatactttga
     7081 taggtaaatt atctggtggt gaaagaagaa gactacatct tcttaaaatc cttatgacag
     7141 ctcctaatgt gcttctttta gatgaaccta caaatgattt agatattgaa acgcttaaaa
     7201 tattagaaga atacctagac gaatttaaag gtgtagtagt tacagtatca catgatagat
     7261 atttcttaga tagaatttgt aataaaatat ttgcatatga aggtaatgga aaaattcata
     7321 tctatacagg taattacagt gattatctta tttatagaga gattcaagga attgaatttg
     7381 aagaatctaa aagggaatca gttaaaaaag atgactctac taccctaaaa aaagaaaaac
     7441 ctaaaaatga taaaaagctt aagttctcat ataatgaaca aagggagttt gaaaatattg
     7501 actatgatat agaaaaactc gaatctaaaa ttgcaacact tgatgaaagt actgcaaaat
     7561 tttcaactga ttttactaaa cttcaaaaaa ttttagatga gaaatctaaa ctagaaaaag
     7621 aacttgaata caaatatgaa agatgggaat atttaaataa tctagctgat gaaatagcta
     7681 ataataagta atattaaaat aatcttaagt ttaataagtt ttgctttaaa tctgtaattt
     7741 ataaatattt taaaggaagt gtctcaaaat gaacttttta gttttatgag acactctttt
     7801 ttatg
//
LOCUS       DAEMHW010000169         7801 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEMHW010000169 DAEMHW010000000
VERSION     DAEMHW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.169"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            53..730
                     /locus_tag="KPK67_003018"
     CDS             53..730
                     /locus_tag="KPK67_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459950.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(1059..1826)
                     /locus_tag="KPK67_003019"
     CDS             complement(1059..1826)
                     /locus_tag="KPK67_003019"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG2459951.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(2275..2475)
                     /locus_tag="KPK67_003020"
     CDS             complement(2275..2475)
                     /locus_tag="KPK67_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2459952.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEERYQILFRESI"
     gene            complement(2477..2884)
                     /locus_tag="KPK67_003021"
     CDS             complement(2477..2884)
                     /locus_tag="KPK67_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG2459953.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            complement(3244..3693)
                     /locus_tag="KPK67_003022"
     CDS             complement(3244..3693)
                     /locus_tag="KPK67_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2459954.1"
                     /translation="MELIEWNYAYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFI
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKRIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRESSKVLEKCGFKIEEYCHNS"
     gene            complement(3707..4633)
                     /locus_tag="KPK67_003023"
     CDS             complement(3707..4633)
                     /locus_tag="KPK67_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459955.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(4758..5540)
                     /locus_tag="KPK67_003024"
     CDS             complement(4758..5540)
                     /locus_tag="KPK67_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBG2459956.1"
                     /translation="MTKTQQEMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVATHEYESC
                     VNALQATINLYLELREELSQSPVVRKVEAERVALQYFQDLIK"
     gene            complement(5696..6316)
                     /locus_tag="KPK67_003025"
     CDS             complement(5696..6316)
                     /locus_tag="KPK67_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2459957.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(6415..7499)
                     /locus_tag="KPK67_003026"
                     /pseudo
     CDS             complement(6415..7499)
                     /locus_tag="KPK67_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            7496..7769
                     /locus_tag="KPK67_003027"
                     /pseudo
     CDS             7496..7769
                     /locus_tag="KPK67_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         2668 a         1285 c         1056 g         2792 t
ORIGIN      
        1 ttagaccatg ttctatggcc ttattttaat ataaaacgat tggagatagg ctatgattgt
       61 gattgaaggt agcgataaat ttaagattgc aaaagaatat attgatgtag aatatactct
      121 ttttagcaaa gtaactttta ggtatgaaaa gttgaaattt aaagataatg ctgaattgga
      181 aaaaattaag atgtttaaat ataaaaatgg ctacatccct aataaattaa acctttcttt
      241 tggatatgga ttctcttctt ataaaaagca aataattaga gaaactgtag atactttaag
      301 attgacagaa attttttcaa gcgagaacat agaagatatt aaatttataa aagatggtac
      361 aaaaaaatta gaaattagca tagagaaagt tgtgaaattt aaacgtcgaa aaaaaagaaa
      421 ttatgtttgt tgctattgcc ctgatatgta tagggacata aaactcgaca aagaatctat
      481 caataagata tacaacagaa aaataaaaat agaaagagaa gttaatattt ttgaagatga
      541 ggatgttata ataaacaaga aagtattgaa gtttccaaag tcttggacaa agaatatgca
      601 aaaatattgg ttaagtgaaa ataagtatcc catacattct actgtaattg atgatgatag
      661 atataaatgt tgtaatgtac aatatacaaa aaatagagtg ataatattat attacatata
      721 taaccattaa tatacataat tacaaaaaaa ataatgacaa atatacataa tacaaatata
      781 tcttctactt tagataaaac agttattatg aaaacttact atctataaat aatagaaatg
      841 ctagtatacc atctataatc taatgtctag taggttttaa tggcatattt taaatatgcc
      901 atagttgatt tataagattt tctatatcac tttcaaaata cacactaaat agttgctttt
      961 cagatataat tgcccaattt aaattttact atctgaaaag ttttttatat ttaatttata
     1021 tcttcaaaaa taccgtctaa tatttctata taattaattt aattaatact cttatgtaat
     1081 agattaagta ctgaatttaa ctctttaaat gatatttgtc ctggtgctga aacacttttt
     1141 gcagctccaa aagtcaatgc agaaccaaat atttcaccac acaatctact tataacaccc
     1201 attcctgaca ttgacattgt tattattggt ctatctgcat aaattttaaa catttcattt
     1261 gtagcttcaa gtagcactaa cacatctttt tcattttgtg gcataacagc tattttgggt
     1321 aaatctgcgc ccaattcctg cattctacat aaacgagata caatctcttc tttttttgga
     1381 gttttattaa aatcatgatt agatataatt actttcactt cttttttatg agcaaaatta
     1441 actacttcat ctatgacctc atctcccata aataactcta catctattaa atctactaaa
     1501 ccggtattag aaatttcttt attcaaagta gtataataat ctcttgatat taatttttct
     1561 cccccttcta ctacacttct aaatgtgaat aataaaggga tatcatgtat ataacttctt
     1621 aattcataga gaacttcctt aacttcttta atattttcta cattctcaaa aaaatcaact
     1681 cgccactcta taatatccaa acatgcatct tttaactctt tagcctcttt tataatatct
     1741 tttttatttt tcccaataat tggtacacaa attttaggtc taccttctcc aatagtaata
     1801 ttttttactt gaacttttct tttcatattc tcctccataa tcagaaattc tataggactt
     1861 taaattctat tttctgcaca atttaattat acattaaaaa tactatacat tataaaaata
     1921 ctaatttaaa aataattttt attctaattc ttaaaacaat cccacatatt ttagaaaagg
     1981 tactctataa tatacaaaca cttctttttt acctaaattt attttaacat tctataattt
     2041 tcatagtatt ctttaaattt tattcatata aacaatatta ttctggatgc taatagaatt
     2101 aatctataaa tcatagtata attatgcaga aataactcga atttcactac ttatgaagag
     2161 attgaatatt aatgaatcta aattatttgc attacttaag agttttgact aaagttgaac
     2221 accatacaaa agtagctact caattgttta taccccaacc aagcttaagt aatgttatat
     2281 agattctcga aaaagaattt ggtatctttc ttctacatga atatagggat ttccgtattc
     2341 atcaaataaa gttttctgtg aaagacgaat gatatagtgt tcaaaatgtt taagtattcc
     2401 gtttattgca tttacatcgc catgtacagc aagtaaaata attgtaaaag gtaatacttc
     2461 tttagtaggt ttcatttcaa tgttcctcca tgagttcttt caactctttt agtgcatttg
     2521 ttttgtgata atgtatggta ttttctgcta aatccatcaa tgttgctatg tcagcatttg
     2581 tcatgttgag aaagaatgaa agtagaatta tatcttgttt tctttttgta agttttccga
     2641 ttgcttgcgc cattaataca tcttcaattc gtatgttgta gtcgtaagaa accatacaga
     2701 tgtagttaga atcataatca tctaaaatac tcaatttact taattctgca agtgacaaag
     2761 attcgagaga taccaaataa tcatttctgc gtttattttc gtcataaata tctctagcat
     2821 agttacgcaa aactgtttta cagaaactgt cgaattgatg ttcaattgtc ttttcatcaa
     2881 ccataagatt tcaccctctt tccatcgcaa ggagttaaat aaccttgctt actcctatca
     2941 cggtgggagg gcgttaaatc ttcgataaat ttcaaaaaaa agagaaaaaa atacacactc
     3001 tgcctatagg gcaaaaatgt gtattcttta aaacgtattt aatttatatt ggttgtaagc
     3061 caccaccata ttgatgtatg gaaatatgca ttggctagct tttcataaaa atacaaagat
     3121 gcataagaaa ttaatagagg ggaaaaactt attctgttat taaaaagtag gttaattcta
     3181 ataaatgcta ttgcggtcga attaaactat atacatggca atcaatcatg tttctattct
     3241 ttattatgaa ttgtgacagt attcttcaat cttaaaacca catttttcca atactttact
     3301 tgactctcta ttgtaggaat atacattagc atgaattttg ttaaggttgt atttttcaaa
     3361 ggcaattttg catatttctt ttactgcatt tgttgctatc cttttacccc aatagttcct
     3421 agcaagccag taacctaatt cagcattttt attaatagag gtatcttctt caaatgttag
     3481 gctgatactt ccaatagccc tttcttgata aaaaatagca tagctaagtt gtttaacatc
     3541 tggagttgcc aaacaaaaac taataaaatc tctagcgttt tgctccctat aagggtgggg
     3601 gaagttgtca cgaagattct ttgcaatttc tttatcattc gcaaactcaa ctaaatcggt
     3661 aatataagca taattccact ctataagttc catttttctt ctccttttat gaaacagcta
     3721 caagaaatct ttctaagcca ataccactta tcaaacagcg ttctttttta tttccaagca
     3781 tttgataagt ccagtcaaca aatccaccgt caactatttc aacggtttca tttccttgtt
     3841 tgaccgataa tttgaaattg atacctttat aataattatt atcaacatca tcattatgaa
     3901 ttgataactc tatattagga aaaataagtt tgatagtttc aaccattctg tcgaaaaatc
     3961 cgtcattatc tttgtatccg tttcttttgc gtagaataat agagaaacga acattttcta
     4021 tttcacttaa tatatcttta taatagttta agtgcttttc taacaacatt ttttcacaag
     4081 tgtaagaccc tgtatctata ccagatgaaa ccatacaata taagccaaaa tgtgaatata
     4141 aacctttacc tacaaaattt tgtgctctta ttaccctagc agtagtagcc atgtggatag
     4201 gtatggtatt attttcttct tttcttttta atttatcagc aataattatt gccaacatat
     4261 ttgaggggtc tgcaagtgtt tctgtccctc gtagagcact tactacgtta tattgatcta
     4321 cggagccaaa aacgctacag ctacctaatg gagcagaggg agaaagcata atcgtcttga
     4381 tatccatatt gaatgctttt ctcaataatt ttgattccaa agcatgaatt tcttctggat
     4441 ctataccgct agggatagta aatctattta gttggtatga ctttagaata tcagttgcag
     4501 ttaaagtatt tgcttgtcta tcaaacactt ctaatagaag tgagttcaaa tctgattttg
     4561 atagagctga taacttatca actatattct tatccccaat tttatttgta attctactta
     4621 aaatatcact catagttatc ctccatactg ttactttcaa aataattata gaatggttac
     4681 aacaactaat ctaggtcatt tgaccaaaaa tgaaaaaaca ctgaatattt actattcaat
     4741 atttttttac tttgggttta tttgattaaa tcttgaaaat actgtaatgc aactctttca
     4801 gcttcaacct ttcggacaac aggactttgt gataattcct ctcttaactc aagatacagg
     4861 ttgatagtag cctgcaatgc attaacacag ctttcatatt catgggtagc aaccgtcttt
     4921 tctaatgctt caatatattg gggtgcgtcc aattcaattt ttctaactcc acgaggtaat
     4981 ttacctaaga gaagctgtat taatggggca agtactgatt gtcttaaaaa agataaaaaa
     5041 tcaattgttt caaatagttc tctacgtcca atcttagtgc aagcgtaatg aacccaaatc
     5101 caaaatctat cttcaatcca ctgtaagtct ggagttggga agtatgcttc ttttttagaa
     5161 taaatttctg ttatgcagtt gttttcttca tagagaatag ctgaatcttc aaccctatct
     5221 agtaaaccat ctaaggttgt aaacttaaaa tcaacatgaa gaacagggga atcatagagg
     5281 gaaattatca atcgaggttc tccaacgtgt tcgccagtaa aagcagaaac taatgttcca
     5341 aactgttcca caataagtaa tctctcttcg ataacttctt cataggattt atcatctgct
     5401 acaatcagaa aatccaaatc agaatattca tctaacgatt ttgtgatata agaaccgcta
     5461 attgcaagtc ctattatcct actatctctt tttgaaaatt ctaatacgtt ttgtatcatt
     5521 tcctgttgtg ttttagtcat aatataacac ctctcacttt cttttattag ctatcaatcc
     5581 aacataatta tgcataggag tattatataa tgaagaaata aaaaaaaata ggtcacttga
     5641 cccgaaataa aaaagcattg aataattatt atccaatact tttttgaaat tatacttatg
     5701 ttgtaggaag attctttaat acctctcgct gattaattac actaaatctt ttgttttcat
     5761 aagcaattag atgttcttgt acacattcat ttatagcacg attaatgctt ctaattggag
     5821 cgtttacttc gctagcaagc tgttttttgg taagcagatt taattcactt ctataatagt
     5881 gtaaggatat acacctcaga agtcgttctt taacacttaa agatgataat tcttcaatta
     5941 attcagcatt tacaactaat ttactagata tttctcgaat cagaaatttt gtagcttcaa
     6001 aatctcccct taaccagttt aaaaaatcat ctctatgaag tttatataca ttacaatctg
     6061 tgaaagcgac aatttcaacg ggttttttcc catcattaaa ttgttccatt tctccaaaaa
     6121 aactaccact tctataaaga tgaatcgttg agatgtttcc tttcacattt aagatgaatg
     6181 cttctgcaat accgtcaatc ataagaatta catattcatt ttcttgctca gcaaaaagaa
     6241 tagtctcttt ctttttatac gtttttttaa caagcttctt tttaatatct tcattcataa
     6301 aatcaattaa atgcatattc atccctcctt accaaatctt tgattaaatt atatcatctt
     6361 tcaattagtt aggtaagata gtaaaaatat atttacatag aaactattcc attattattt
     6421 tccagttgtc atttttctgc aaagtaagtt caaactggga aagctgttga caatagaact
     6481 agtaggaata tacgagcgaa ccatttcagt attcaactgt tgtaggtttt cagtcagata
     6541 attcttcaaa cgttcaattc gtttgtcctg tgaatcttgc gatgctctaa ccagtttcat
     6601 agatagcgtt ttaccttgtg ccacctgcaa aaaagaccat gcttcgtcaa ggtcaactat
     6661 cttgaagcta gagcggtcag tatgaataaa gtctaatgca aaggtactaa taacaatcaa
     6721 catagcaaca ctaagaagtt ctattgttgt atattcttca aagctagtgt cggcatctgg
     6781 caatacgagg tcagctgttt ctccaagctg atatactgct tcacatttcc gtcactaaac
     6841 agtagatgtg caaaatcata gtctacaaaa ctttcgatat ggtcggctat gctgttactg
     6901 atagaagtat cttcttttcg taattcatca atgacaagta ataaccctcg ctgttcactt
     6961 tgggttacat tgcggattgc ttttctaagc actgggaact tatcactgtc acgactggag
     7021 ataccagtaa ggaatattag gatatcaatc gctaaacttt cagaatcctt tttacggctc
     7081 aaaatgacgt aagggtcaag caattctttg ttttcatcat cactggttaa attgacaatg
     7141 tttatctcat gggctatttc tggtagcgtt tctttccatt tgccacgttc tgctttaggg
     7201 tcaactatga ccgcttgtcc gccgaatagt accgcatagt aaacaagcat attgttactg
     7261 aatgatttac caccacccaa tgaaccgata aacgcagacg ctaacacatt ggtaatagaa
     7321 ccctttaccc cttgactagc aagggcaggt tttagataga cattctttcc agtgtccaga
     7381 ttgtagccaa catagatatc ctcatgttcg ccaaggtttt gtgtcgctcc aaaaccaagt
     7441 ccagccaaaa aatccgaagt gacatattga atataatcat tgatgtaatg tttgctggct
     7501 aggttatgat tctataatta tgtcaggtgt aaatattggt gatggtgcaa ttattggaac
     7561 aagagcagta gttactaatg acgttccact gtattctatt gttggaggag tacctgcaaa
     7621 aattataaaa aagagattta ataaaaaaga gatttaataa tgatacaatt tcaaaattat
     7681 tagaaattaa atggtgggat tggccatata aaaaaattca atcaaacatt aagcatattc
     7741 aatcaggaaa tattgataaa ttagtttaat attttataat ttatttatat taaactttac
     7801 t
//
LOCUS       DAEMHW010000170         7784 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEMHW010000170 DAEMHW010000000
VERSION     DAEMHW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.170"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            170..364
                     /locus_tag="KPK67_003028"
     CDS             170..364
                     /locus_tag="KPK67_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBG2459958.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(466..975)
                     /gene="vanZ1"
                     /locus_tag="KPK67_003029"
     CDS             complement(466..975)
                     /gene="vanZ1"
                     /locus_tag="KPK67_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBG2459959.1"
                     /translation="MKSKKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLIIFANL"
     gene            complement(1299..1688)
                     /locus_tag="KPK67_003030"
     CDS             complement(1299..1688)
                     /locus_tag="KPK67_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2459960.1"
                     /translation="MNINKLPYTELKLMKYIWRVDDILTTRDVLETMKLKCDWKKSTT
                     LTLLNNLVDKVFLTTYKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEGDIDD"
     gene            1941..2705
                     /locus_tag="KPK67_003031"
     CDS             1941..2705
                     /locus_tag="KPK67_003031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459961.1"
                     /translation="MDNIKNLEYYKNNLELYSNDLMKLVTSLAKDEVLNTNTINYATP
                     CTIWKIIKYFLVGEGYDLSGLKTELKFKENLDFNLNNGILNEISCELESLINSNYIKS
                     CKLKEELKEASKIFKCLIEAIKQVNCNDKCEFLVGKLFCLLVKIILLIVAIIAKIILL
                     LIFCNDCDKCNDDKDVNSYFCKCLICDLEEELNRAEELIDELIKLAIEFIKYSSKKCK
                     YDKCYEDEYCNTCYEKSSYLNKCDYNLHESLDKFKK"
     gene            complement(3147..4316)
                     /locus_tag="KPK67_003032"
     CDS             complement(3147..4316)
                     /locus_tag="KPK67_003032"
                     /inference="COORDINATES: protein motif:HMM:TIGR01766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2459962.1"
                     /translation="MITVKKLKLTIVGNEEIRKEQYKFIRDSQYAQYRGLNLAMGVLT
                     SSYLLSGGDVKSDYFKDAQKSLKNSNKIFNEISFGKGIDSKSSIIQKVKQDFYISLKN
                     GLAKGERGFTNYKRDFPLMTRGRDLKFYEEDKEFYIKWVNKIVFKILTGRKDKNKVEL
                     IHTLNKVLNKEYKVSQSSLQFNKNNNLILNLTIDVKSDVKVEVIKDRVCGVGVGINTP
                     IYVALNDILYISQSIGSNDELIKQKKQFEARKKRVRERVKNKKELNSLKEKERNWINT
                     YNHMLSKRVVEFAKKNKCEYIYLEKINDNEFKNKVLKKWPYCELQKMIRYKAAGFGIE
                     VKYIHYYHTFQKCSRCGYEYNKSIAIQKRFKCLNCGLEVNSDYNIARNISRYDIL"
     gene            complement(4458..5060)
                     /locus_tag="KPK67_003033"
     CDS             complement(4458..5060)
                     /locus_tag="KPK67_003033"
                     /inference="COORDINATES: protein motif:HMM:NF024085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG2459963.1"
                     /translation="MNENISTTGITSLEDYPKGKCKKYYKEFIEDDILCIPTQKPDIE
                     CINEVSAKICIEDKNIINTILGPKLVIQGSKNIKIIYTADNCQQSLHSAHWNIPFCKF
                     ILLEDLCYDKCEDIIESIFIGVENICVKYFDCKMIDLSLLFIICPQICNCNNCKYCRN
                     YCMYNPNLCHYNTDNRLDNCNTTTYKHNISDKYNSKIRYK"
     gene            5249..5821
                     /locus_tag="KPK67_003034"
     CDS             5249..5821
                     /locus_tag="KPK67_003034"
                     /inference="COORDINATES: protein motif:HMM:NF024085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG2459964.1"
                     /translation="MNSNICTCNMDLIDVSGLCNTDDVTSVISQYPYWFQMYIPETLN
                     VPPQKPDIEQINSVNVSVNILRTKVVKTPISPLDANGDFIPNLEGKISTGRKLIIEGE
                     LCQKISYTANEETQSVHSAHFYVPFSSYIVVPQEVTFDNVTPPITLDSLDVDYQVNAC
                     IEDLSISTLDERTILKHTTLLLYAVPMQPL"
     gene            5850..6446
                     /locus_tag="KPK67_003035"
     CDS             5850..6446
                     /locus_tag="KPK67_003035"
                     /inference="COORDINATES: protein motif:HMM:NF024085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG2459965.1"
                     /translation="MLNSNYTNGTSHQFYDKCCGENKILNTVGVYPQLKIEKALQCYE
                     DTDKWTEFFVPEIVDIPSQKPDIEDIIEVHSCVDIISQRVIKTPLVNGYTPVGQSPIP
                     GEDIPNGECTNLTGRKLIIEGILKQKIIYTAAVDEQSIHSAHFNIPFSVFIIIDKDTP
                     LYQQFKITSYIEDIYACALSERSVFKNTTIFIKASKLC"
     gene            6477..7676
                     /locus_tag="KPK67_003036"
     CDS             6477..7676
                     /locus_tag="KPK67_003036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459966.1"
                     /translation="MECNKKTCECDFNIKSIGVCDVTKLNMDGSKRETLNWTEISIPE
                     ILPIPILKPDIEDIDQVYANAVISSTKLIETPFAYKSYNLYVSLDILDEIQTILNTFL
                     AVDIQTSIDAIIDGTNDIISAIQAAIPLIPGLGPIVNPLLIELQGLLNLIQPTVDSLL
                     AAVDELLAAIQIGTAQAVCTALNLVISITTTLINLLESVQNLINDILAAIGTLGGPLV
                     AALIATLQIIINTILTPAFDLLIGATGALTVLLQDLSGILIDCNNTMAFEIIQNAEGT
                     CLTGRKLIVEGLLKQKIVYTALVDEQSVHSAYYEIPFLAYIIPYAKFEGLTYEEGIVV
                     SAPGEPVITINGYRYNSSTGINVDLCEDFVVNACIEDIYVDALDERTIFKNITLFLKA
                     KLESLCN"
BASE COUNT         2967 a         1011 c          898 g         2908 t
ORIGIN      
        1 ataatatact tttttctata aatattttac ccataacctt tattttttta ttaaaatgca
       61 ttataataaa aagtaaatct aaatttgata cagttttaac aatcattatt ttcagaaaag
      121 tgatactatt gaatatatta agaataatta gagatggagg aactaagata tgagcgtaat
      181 agtaatgtgt agtcgttgtg gaaaagaaat agataaatat agaaataaat atataactta
      241 ttatgagaaa aataccaatg cagaaattaa agtttgtata aattgtgcgt tagatttatt
      301 agaaaaaaca agagaaaatc aagaattgaa catacaagag tttcaagata caataattct
      361 ttaataattt aggaaagcac ctgtataggt gctttttatg tgtaaataat tttatatgta
      421 actgtatctt acgttctatt ttaagctaaa catatatact tatttctata agtttgcaaa
      481 aataattaat cctaaaaata atacaacaat aattgtcaca atcaatgcca atacattgat
      541 aatttttatg gtattattct taaatatttt cgataatagc ataaaaacag ctattcctat
      601 gattcctcct agtgtgtttc ctatcaaatc cgttatatca cttgcaccta gtgcaaatat
      661 atattgaagt gtttcaaatg ctaaactagt tataaaaata gggataactt tttttataaa
      721 gctccattct tcttttagca tacacacata tatcccaaat ggtacaaata tagccacatt
      781 taatataatc tctgatatat ctactctatt attaattata agtgacccag caaaaggtac
      841 taagtttatg cttctaagat tcatggtttc aagagatgag atatcaaatt gcaacttaaa
      901 tagtattatc catgttaaaa taattatgta aactataaat aatccttttg taatattatg
      961 ttttttagat ttcatttatt acctccttac tatattattg tattatttta ttaatacaat
     1021 aatatagtaa ggtaatttat ctgtcaacta tttcttctaa aattatattc cttttataat
     1081 ttcaatatct ttatcatatt tacatgattt aagctattgt agtatttcat atctttcttt
     1141 aaattttaat tggtattatt ttctatattt tttttactat cagatactaa ggaaataagc
     1201 acttttcttt atataatgtt ctaccatcac ataacactaa cattaaatca aaacgctact
     1261 ctcaattgtc tcaaactaaa aagatactga taactttatt agtcatcaat atctccttct
     1321 ttcaaatttt taaagtattc ttctaacttg tccaagctct tactatctaa aacttcatca
     1381 tcatgtaatg ctgaaataaa actcttaaat gaattgttat gcataaaact aaaaaaactc
     1441 tttgtctcaa cttttaaata atctttctct tttatagcta tagtataatg cgtacatcta
     1501 tctaccttat aagtagttaa aaatacttta tccaccagat tatttagtaa tgttaatgtt
     1561 gtgctctttt tccaatcaca ctttaacttc atagtttcta ggacatccct tgtggttaat
     1621 atatcatcta ctctccaaat atacttcatc agttttagtt ctgtatatgg taatttatta
     1681 atattcatta gtttgcacct attgtcaata tttatatatt taaatagtat aacaatacat
     1741 tttataatca attagttcct ttaatttatt taaaattatt gccctataaa aaggatattt
     1801 tacacaagtt gaataaaaaa caatattttg tatataattg taaaatttta tttttatttg
     1861 tatcataaat aaatatacac catatatata tattaggata ttaaatttat cctaatatat
     1921 atatatagga gtggtaaata gtggataata ttaaaaattt agaatattac aagaataacc
     1981 tagaattata ttctaatgat ttaatgaagt tagtaacaag tttggcaaaa gatgaagttt
     2041 taaatacaaa tacaataaat tatgcaacac cttgtacaat atggaaaatt ataaaatatt
     2101 ttttagttgg tgaaggttat gatttatctg gtttaaaaac agagttgaaa ttcaaagaaa
     2161 atttagactt taacttaaat aatggcatat tgaatgaaat ttcttgtgaa ttagaatctt
     2221 taataaattc taattatata aaaagctgca aacttaaaga agaattaaaa gaagcttcta
     2281 aaatatttaa gtgtctaatt gaagctataa aacaagtaaa ttgtaatgat aaatgtgaat
     2341 ttctagtagg aaaattattt tgtttattag ttaaaatcat acttttaata gtagctatta
     2401 tagctaaaat aattctacta ttgattttct gtaacgattg tgataaatgc aatgatgata
     2461 aagatgttaa ttcttatttt tgtaaatgtt taatttgcga tttagaagaa gagttaaata
     2521 gagcagaaga attaattgat gaattaatta aattagccat tgaatttata aagtatagtt
     2581 caaaaaaatg taaatatgat aaatgttatg aagatgaata ttgtaatact tgctatgaaa
     2641 aatcatctta cctgaataaa tgtgattata atttgcatga atctcttgat aaattcaaaa
     2701 aataaaatta tagaaaaaaa tataataaaa attacaaaga ataatataat aatattgttt
     2761 tgaattgaaa tattttgata attaaatgta tagactaata tgaatgatgt aaattttatc
     2821 taatttaact attttatagc aaaatataga ctatattaac aagaggttat tttaatagat
     2881 ttaagagtct aaaagaatta acctcttgtt ttgcaattaa taaataatat attacagcaa
     2941 taagtaactt tttcctgtaa ttgctcctgt atttacacac tacaaatata ttataaaata
     3001 tacataatgt gtctagttgc caacataaac tagagttttt tcggcttgaa aagcaactat
     3061 ggtatacaat tggtaaatta accttatata ttttttccat aatcctttca ttcggacact
     3121 aaattgcccg ttattctgct tttatcttac aaaatatcat atctagatat atttcttgct
     3181 atattataat ctgaattaac ttcaagtcca caattcaaac atttaaatcg tttttgaata
     3241 gctatacttt tattatactc atacccacat ctactacatt tctgaaaagt atggtaataa
     3301 tgtatatatt taacctctat tccaaatcct gcagctttat atctaatcat tttttgaagt
     3361 tcacaataag gccatttttt taaaacttta tttttaaatt cattatcatt tattttttct
     3421 aagtaaatat attcacactt attttttttc gcaaattcaa ccaccctttt gcttagcatg
     3481 tggttatatg tattaatcca gtttctttcc ttctctttta atgaatttaa ttctttttta
     3541 tttttcactc gttctctaac cctttttttt cttgcttcaa actgtttttt ttgctttatt
     3601 aattcatcat ttgaccctat actctggctt atatacaaaa tatcatttaa agctacatat
     3661 attggggtat ttatacccac cccaacacca catactctgt cttttattac ttcaactttt
     3721 acatcagatt taacatctat agtgagatta agtataagat tattattttt attaaattgt
     3781 aaagaacttt ggcttacttt atattcttta ttcaaaactt tatttaaagt atgtattaat
     3841 tcaactttgt ttttatcttt cctaccagtt agaattttaa aaactatttt atttacccat
     3901 tttatataaa attccttatc ttcttcataa aattttaagt ctctacctct agtcattaaa
     3961 ggaaaatctc ttttataatt agtaaaacct ctttctcctt ttgcaagtcc attttttagt
     4021 gatatataaa agtcttgctt taccttttgg ataatagaac ttttactatc tattcctttg
     4081 ccaaagctta tttcattaaa aattttattt gaatttttca aacttttttg tgcatctttg
     4141 aaataatcag actttacatc tccgccactc aataaatatg aacttgttaa tacacccatt
     4201 gctagattaa gacctctata ttgagcatac tgactatctc ttataaattt atactgttct
     4261 ttccttattt cctcgtttcc aactatagtt aattttaatt ttttaactgt aatcatagca
     4321 atcatcctta acataaaatt taatgatgtt atagtatcct ataatttatt atatttatac
     4381 ttatataaaa ttatgataat tataattaaa aaaaagacaa taaatatatc tgaatatact
     4441 tattgtcttt tattttacta tttatatctt attttactat tatatttatc actaatatta
     4501 tgtttgtatg ttgtagtatt acagttgtcc aaacgattat cagtattata atgacataaa
     4561 tttggattat acatgcaata atttctacaa tatttacagt tattacaatt acaaatttgt
     4621 ggacaaatta taaataatag tgataagtca atcattttac aatcaaaata ctttacacaa
     4681 atattctcta ccccaataaa tatagattct attatatcct cacatttgtc ataacataaa
     4741 tcttccaata gaataaattt acaaaatgga atattccaat gggcagagtg aagagattgt
     4801 tgacaattgt ctgcagtata tataatctta atatttttag aaccttgtat aactaatttt
     4861 ggacccaaaa tagtattgat aatattttta tcttctatac atatttttgc acttacttca
     4921 ttaatacatt ctatatctgg tttttgtgtt ggtatacata atatatcatc ttctataaat
     4981 tctttatagt attttttgca cttacccttg ggataatctt ctaaagatgt aattccagtt
     5041 gttgaaatat tttcattcat acaaacctct ccttaatttt ataattatat agagattaaa
     5101 gtttctatat acatttatat atgatataaa gtcgtatttt gttataaaaa atataaaaaa
     5161 aacaaaaata agtaacaaaa aatcgaaaat taaatacatt atattgagag ctaaaagttc
     5221 tcaataaatt atataaggag agataaatat gaatagtaat atatgtactt gtaatatgga
     5281 tttaattgat gtaagtggct tatgtaacac agatgatgtt acaagcgtta taagtcaata
     5341 cccttattgg tttcaaatgt atatacctga aaccttaaat gtaccaccac aaaaaccaga
     5401 tatagagcaa ataaactcag taaacgtatc agtaaatata ctaagaacaa aagttgttaa
     5461 aacacctata tcacctttag atgctaatgg agattttata ccaaatttag aaggaaaaat
     5521 atctacagga agaaagctta taattgaagg ggaattatgt caaaagatat cttatacagc
     5581 taatgaagaa actcaatcag ttcattcagc acatttttat gtaccatttt caagttatat
     5641 agttgtgcca caagaggtta catttgataa tgtaactcca ccaataacat tagattcact
     5701 tgatgtagac tatcaagtaa atgcttgtat agaagattta tctataagca cattagatga
     5761 aagaactata ttaaagcata caacattatt attatatgca gtaccaatgc aaccattata
     5821 aaatttataa aaaataagga gtggaaaata tgttaaattc taattataca aatggaacaa
     5881 gccatcaatt ctatgacaaa tgttgtggtg aaaataaaat attaaatact gttggtgttt
     5941 atccacaatt aaaaatagaa aaagcattac aatgttatga agatacagat aaatggacag
     6001 aattctttgt tccagaaata gtagatatac catcacaaaa gccagatata gaagatataa
     6061 tagaggtaca ctcttgtgta gatataatct ctcaaagagt aataaaaacc cctttggtaa
     6121 atggatatac tcctgtaggc caaagtccaa taccaggtga ggatatccca aatggagaat
     6181 gtacaaatct aactggaaga aagttaataa ttgaaggaat attaaagcaa aaaataatat
     6241 atacagcagc agtagatgaa caatcaattc actctgctca ctttaatata cctttttcag
     6301 tatttataat aatagataaa gatactccat tgtatcaaca atttaagata acttcatata
     6361 tagaagacat ctatgcatgt gcattatcag aaagaagcgt atttaaaaat acaacaatat
     6421 ttataaaagc atcaaaatta tgttaagaga tttaaataaa atgaggagga ataaaaatgg
     6481 aatgtaataa aaaaacttgt gaatgtgatt ttaatattaa aagtataggt gtatgtgatg
     6541 taacaaaatt aaatatggat ggaagcaaaa gagagacttt aaactggaca gaaatctcaa
     6601 ttcctgaaat acttcctata ccaatattaa aacctgatat agaggatata gatcaagttt
     6661 atgcaaatgc agtaatcagt tctacaaaac ttatagaaac tccatttgca tataaatcat
     6721 ataatctata tgttagttta gatatattag atgaaataca aacaatacta aatacttttt
     6781 tagcagtaga tatacaaaca tcaattgatg caattattga tggaacaaat gatataatct
     6841 cagcaattca agcagcaata cctttaattc ctggtttagg gccaattgtt aatcctttat
     6901 taattgaatt acaaggattg ctaaatttaa ttcaaccaac agtagactct cttttagcag
     6961 ctgttgatga attattagct gcaattcaaa ttggaacagc acaggcagtg tgtacagctc
     7021 ttaatttagt aataagtata acaactactt taattaactt actagaatct gtacagaatc
     7081 ttatcaatga tatacttgct gcaattggta cacttggagg accactagta gctgcattga
     7141 tagcgacttt acaaataata attaatacta ttctcacacc tgcgtttgat ttattaattg
     7201 gagcaactgg tgcattaact gtactacttc aagatttaag tggaatatta attgattgta
     7261 ataatactat ggcatttgaa ataatccaaa atgctgaagg aacatgctta actggaagaa
     7321 aactaatagt tgaaggttta ttaaaacaaa agatagtata tactgcacta gtagatgaac
     7381 aatctgttca ttctgcctat tatgaaatac catttttagc gtatataatt ccttatgcta
     7441 aatttgaagg tttgacatat gaagaaggaa tagtagtttc agcacctgga gagccagtaa
     7501 ttacaataaa tggatataga tataattcta gcacaggtat aaatgtagac ttatgtgaag
     7561 attttgttgt aaatgcttgt atagaagata tatatgtaga cgcacttgat gaaagaacaa
     7621 tatttaaaaa tataacatta ttcttaaagg caaaattaga atcactatgt aattaatatt
     7681 actttattag tatattgtat aaatttaatg tatatatcct aatggaggat aagaaccatc
     7741 ttaaagtaaa aattttatca ttaaaaagca acctttcata cgat
//
LOCUS       DAEMHW010000171         7770 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEMHW010000171 DAEMHW010000000
VERSION     DAEMHW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.171"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(271..2550)
                     /locus_tag="KPK67_003037"
     CDS             complement(271..2550)
                     /locus_tag="KPK67_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2459967.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTMYILFIQKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2821..4275)
                     /locus_tag="KPK67_003038"
     CDS             complement(2821..4275)
                     /locus_tag="KPK67_003038"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBG2459968.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVNTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4439..5308
                     /locus_tag="KPK67_003039"
     CDS             4439..5308
                     /locus_tag="KPK67_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2459969.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDANSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDIFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5521..7593)
                     /locus_tag="KPK67_003040"
     CDS             complement(5521..7593)
                     /locus_tag="KPK67_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2459970.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PVLANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENTLVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
BASE COUNT         2639 a         1290 c          820 g         3021 t
ORIGIN      
        1 ttttattttt ttaaatatgt ataacacacc tatagaaatt actattatta ctatctaaag
       61 tattttgcta taatcccata tactcttacc tcaaaaacat tcaaatgtaa aaaatactac
      121 atattaataa actttgccca ataattacta aaataatatc ctaacaatga tttatgtttt
      181 tataatagta tatttttaca taaaaaagtg aacattgatt aacaaagttc acttttttat
      241 aaatttttat gttataattc acaattttat ttaatttgtt tcttttctat ttaaactata
      301 tctatctaat attaattttt catattcttc tatcggcatt ggtctagcaa ataaaaatcc
      361 ttgggccata tcacaaccta taaaccttaa aaactctgct tgctctacag tttcaacacc
      421 ttctgaaaca acttttatat caagttgctt tgccatttga acaatacttg aaactacaat
      481 ttgactcttt tttgcatttc ccactgttat aaagaattgc ctatctaatt tcaatacatc
      541 aattggcata tctttaagca tatttaaaga agaataacca gaaccaaaat catccataga
      601 aattttaaat ccaacagatt tcaaagtttg cattatctta aataatattt ttgtattgtc
      661 aaatattgca ctttctgtca attcaatctc aattaaattt ggttcaattt catattcttt
      721 tgttattctt tcaagctcag aaataaagcc tggattctca agatgtactc ttgataagtt
      781 tattgatata ggaaatgtgg ggaaattttc attttcccaa cgtttaaaat ttttacaaac
      841 ttgggtaaat acaaacatat ccaattctgt tataaaacca tttttctcaa aaataggaat
      901 aaatacacct ggacttatta gacctttttt gggatgttgc caacgtacaa gtgcttcagc
      961 acctacaatt ttagtagttg ataaatcaac cttaggctgt aaataaactt taaattgttc
     1021 attttttaaa gcatcatgca tttcttgttc aatatcacgt tcttctatga gagtttcttt
     1081 taaattttca gcataaatct cataaacttt taaatatttt ctttttactg tagtttttgc
     1141 aatcatagct ttgtcaagac aggtagaaat atttgtttct ccctcttcaa cgaaatatac
     1201 acctatggat ggaattaaat tgagttctac tcccaaactt gtttttatca ttgacaattt
     1261 tttacgtata agttcacata tttctataat tgactctcta ttatttttct tttcaaatat
     1321 tatagcaaaa ttgtcatttg aaattcttga aaatacagat tgctctttaa atatattctt
     1381 aattatctct gctatttcct ttaatactct atctccttct tcatacccaa acctatcatt
     1441 tactagctta aacttatcta tatcaaaata aattaaaact agattctttt tctcatgaga
     1501 ggataaaaat tttccccctt caagattaaa tttatttgaa ttaccaatac ctgtaacgtt
     1561 atctttaaaa gcaaattctt ccaatttttc cttactcttt ttctgtataa ataatatata
     1621 cattgttaat gccgtaaata ttataatcaa gactacacat gctgataatg taagttttat
     1681 aatagcgtat gatttctcag atatagcagt tttaggaact tcagaaaaaa gataccaatc
     1741 attgattcct attggggcat aacttaaata atatcctttt ccaaacatac tataacttaa
     1801 tgaacctgat ttaaagtttt ttatatttga cttcatatta ttgatactag attttgatat
     1861 tgaatcactt ctaccaatgt aagaaaataa attttcttta gtagagttgg cgtttttact
     1921 gcttggattt aaaacaatat caccattact tctagttata taagaatatc cttttccatt
     1981 aaatgtaggt acagaaagta ttttttcata caacttcgta ctatgtgtgg caaatataac
     2041 tcctttaacc tcattatctt tataaattgg tacagaatat acattaatat tactattggt
     2101 atttttatca tcttctatta catcttttaa ttttcctgtt ataaccactt ctccttgcat
     2161 tgattttaag aaataatctc ttttagagaa gtcttctata taaccatctg tagtgtatgc
     2221 tgtaccattt ggtaaaataa ctcccattct tttaaatgaa ctattaatag cttgtctttt
     2281 tagtatagaa aggatattat ctacttcaaa agactcttct ccttcaataa atattgaaat
     2341 tgactctagt aactttatat ctccattaat ttgttttttt aacacattga cgctttgatt
     2401 tgctatttct gaaagatatt gattcgtttc ttcacctaat gtcttattca accttgaacc
     2461 ataaatcaaa caggaaattg taattactaa gatacttatg acagcaatta tggacaaacc
     2521 atacctatta ttttttgcat cacttaacat tatgcttcct gcctttctcg aatctatgta
     2581 tgtataatca ttatcttatg acaattattg taaacatttt ataataatac ttatttttcc
     2641 atatttactg taattataca aaattataaa ctttattaca acaataaatt aattaatata
     2701 gatattagtg tttatattat tgcattatct attttacaat tatttacata taaaaaaatg
     2761 taatatcaaa gtgaagcttg atattacatt tttgttcttg tattttgttt aatttttatg
     2821 ctacatctta cttaaacact catttgcaac ttctttatca tcaaaatgat gcttaacttt
     2881 acctataatt tgataagttt catgtccctt acctgttata attacaacat catccttttt
     2941 tgccatactt atagcttctg ctatagcttg ttctctatca acaacaacat ggtaattttc
     3001 ttttttcttg tcaattcctt caagtatatc ttttattatt gcctctggtt cttcagttct
     3061 tggattatca gaagttataa tacataaatc agaatatttt tgtccaattg ctcccatcaa
     3121 aggtctcttt tcagtatctc tatctccacc acacccaaac acagaaatta tttttccttc
     3181 tgcaaattgt tgagttgtct ttagtacatt ttcaagtgcg tcaggagtat gagcatagtc
     3241 tacaattacg cttatacctt tatcatttgg aactgtttca aaccttcctg atactccatt
     3301 agaaagtcta aggccttctt tatatattgg tttaggtatg ccaagtacat agcaagcagc
     3361 tataactgct aaagtattat atactgtaaa cattcctgga actggtataa atattacttc
     3421 ttcatatgaa ggtgttatta atctataaga tactccatcc gaatctgatt ttatatctct
     3481 agccataaaa tcagccttag tattaacacc ataagtgtaa caaggtacat ttataccttt
     3541 tatattttca tatatctttt ttccaccttc atcatctatg tttattatat ttgccatagt
     3601 agtcttaaaa aataattttt cttttgcttt tctataatcc tctaaatctt tatgaaaatc
     3661 taaatggtct ggtgttaaat ttgtaaatat gcctagttta aagtctgttt catcaactct
     3721 atttaatgcc aatgaatgtg atgaaacttc catcgcacag tagtcacatc cattatccaa
     3781 catattatta aaatgatatt gtaaatctat actttctgga gttgttgaat tggaaacaat
     3841 gtctttttct ccatcaaaaa tttttatagt tcctataagt ccaactttat tcttatttaa
     3901 agttaatatc tcatttagta tagtagttat actagttttt ccattagtac ctgtaactcc
     3961 tataacgtta aatttttgag atggatgatt gtaaaaatta tcagctactt ttgccatatc
     4021 ttttcttgta tcttttacct ttataaatgt acatcctttt atttctacat cttcttcaac
     4081 aagaaatgca cttgctccct tttctatggc atcttttata tacttatgac catctgacac
     4141 aaatcctttt atacatataa ataatgtacc ttttgtaacc tttcttgaat catattgtac
     4201 attattaata tctatattta attcaccctt gacattgatt atatccaacc cctgtataat
     4261 atcatttaat ttcataaatg ctttcctccc tttaaactct ttctgctaaa attacactac
     4321 atataatatt atattaagaa ttttttacta tgtgaatggt taaaaatgct tgaaacattg
     4381 caaaaaatgc tctataattt tctataatat aactgaggaa aggtcttcta taaaaactat
     4441 ggataataaa atcgggtact taaacgataa ctttaaaata tttaatatta gagataaaaa
     4501 agatatacaa tttgaatacc ataatcatga ttttaataag ataattatat ttataaatgg
     4561 aaatgtaact tattttgttg atggtattcc ctataaacta aagccttggg atatactctt
     4621 tatcagcaat aatgaaatac ataaaccagt tatagattca aatgtctttt atgagagaat
     4681 tgcaatatgg attagcccag aatttatgaa aaaaaatagt gatgctaata gtgacttaga
     4741 aaaatgtttt aaaatagcta cagagaataa atgcaataga ttgcgtttaa atatacatga
     4801 tattgaatct attaagagtt ttattgagca aataaatatt tccgaatcca atactgaatt
     4861 tggaaatgaa atattgagaa atacattatt tatacaactt atgatattct taaacagaat
     4921 tttcctaaaa aatgaaaata tggaatcaac agaagatatt ttttatgatg aaactattaa
     4981 gaaagtatta aactatataa attcaaatat agatgataag ctttcaatag atagcatatc
     5041 gtctgaattt tttataagta agtattatct tatgagaaaa tttaaatctc atactggaac
     5101 gtctatacac aattatatag ttcaaaaaag attaattttg gcaaagtcac taataaccaa
     5161 tggtcacctt atgagtgaag tttgtaataa atgtggattc aacgattatt ctagttttgt
     5221 aagggcattt aaaaaaaact acggggtatc tcctaaaaat tatataaaca ataaaaatcc
     5281 tttggatatt actcttttag atgaatagat aatttatgta aattttattt taataaaact
     5341 tgaagtatta gtgtatttat cttactaata cctcaagttt tttaatgttt taaaatatat
     5401 caaaatataa tgatatatat ttctattact taaaatatat attaaataaa ttgcatgtta
     5461 actgaaccta tttatatatt tattaaatct cacataactt ttagttacac tatcatatag
     5521 ctatcctatt ttttcaatat catctcttaa tatacttact ctattctcct cttttgctgt
     5581 ggttactcct tttttaatgc ttaatttctg attttgttct aaattcgtaa atactacagg
     5641 gcttattgtt gatagaccat ttttttcaat atattctaag tccattttaa ctaatttttc
     5701 tcccgctttt acttcatctc ctacattcac aaatacttca aatccatttc cttctaaatt
     5761 tacagtttct atccctaaat gtataagtat ttctactcca cttttttggg attttattat
     5821 tattgcatgc ttagttggaa atataactcc aatagttcca tcaactggtg aataaatcaa
     5881 tccatcacta gatttaattg caaatccatc ccccatcagt ttacttgaaa atacagaatc
     5941 tggcacttct tctatatcta atatctctcc atttgttaat gaatcaatct tttctactaa
     6001 agtattttcc atatttgcaa cttccatatt ttttttcact ttatttttat caacattttt
     6061 agtaacagta ctttcaacct ttgtttttcc ctctattata tcttttatat cagactttat
     6121 tgaatctgat tttggtccaa atatagcttg aacattgttg ccaataacca taacacctgc
     6181 tgctccaagt gatttcaatt tttctttatc tactttattt atatcattta caaccaatct
     6241 aagtcttgtt atacacgcat ctaggctctt taagttgtct ttatttccta aagcaatcaa
     6301 aatctctgat gctaattcat tcttagacat ttcaatgact tcagcgttag tttctgactc
     6361 atcttctctt cctggtgtct ttaaattaaa ttttcttatc ataaatctaa atacactata
     6421 gtataatact gcaaaaccaa tacctaccaa gatagccaag taccatggag ttctattagg
     6481 aagaacacca aataataaga aatctattaa tcctcctgaa aatgtgaatc caattttaac
     6541 tcctaataat tgcataattg cgtatgataa acctgaaaat aaacaatgta ttgcaaataa
     6601 tactggtgca acaaataaaa acgcaaactc tattggctcg gttattcctg ttaaaaaaga
     6661 ggttaatgct gctgatgcca ttatacctgc tacaaatttt ttcttttctg gctttgcttc
     6721 atgcaccata gcaagagctg ctgctggaag accaaacatc ataaatggaa atgcacctgt
     6781 cataaaagta cctgctgtga aatttactcc atctttaagt tgagcaaaga atatcttttg
     6841 gtcacccatt acaagctcac cagctttatt tacatattct ccaaattgat accaaaatgg
     6901 attatggaaa acatgatgca aaccaaatgg tattaatagt ctttccaaag aaccttccat
     6961 aaatgctgct agtgtttgat ttgcttctat aacatttttt gagaaactaa gtaatccctc
     7021 ttgtattgga ggccaaataa aagacaatat aattcctatt gctatagcaa ctaatgaagt
     7081 aacgattggt acaaatcttt tacctgcaaa aaaacctaga tactgaggta attcaatttt
     7141 ataaaacttt ttataaagat aagcagccac tatccctata ataataccac caaatacccc
     7201 tgtttgaatt gttggtattc caacaatatt tgcatacata gaattgtcta ccatatcttc
     7261 tgtaacaccc gccataacac ccatagatgt attgagaatt aaaaaaccta ctatggctgc
     7321 caatgctgca acaccttctc cttcagttaa accaacagct accccaactg caaatataag
     7381 tggtaagtta ttaaatataa tttgacctga actttccatt atacttatta taagttgaaa
     7441 tgtaggattc gccaaaactg gaaaatgagc caacaatgcg tctcctgcta aagcattact
     7501 cacgccaagt aatatacctg ctgcaggaag taatgcaact ggtagcatta gagattttcc
     7561 tactttttgt aaaacaccaa atactttttt catttacttt tctctcctta ttattttata
     7621 tttttttaaa taaaaaagac ttaagtttat aaaaagttac attagtatac ttaattctaa
     7681 aattaaactt atgttaatat aaataaacct atattaacct aatttcagaa ttaaaaataa
     7741 actattataa tttttttata aacataagtc
//
LOCUS       DAEMHW010000172         7744 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEMHW010000172 DAEMHW010000000
VERSION     DAEMHW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.172"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            413..1234
                     /gene="yidA"
                     /locus_tag="KPK67_003041"
     CDS             413..1234
                     /gene="yidA"
                     /locus_tag="KPK67_003041"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBG2459971.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYVQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            complement(1371..1520)
                     /locus_tag="KPK67_003042"
     CDS             complement(1371..1520)
                     /locus_tag="KPK67_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459972.1"
                     /translation="MNFFWTKSDFDAWTNEVGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(1527..2012)
                     /locus_tag="KPK67_003043"
     CDS             complement(1527..2012)
                     /locus_tag="KPK67_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459973.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            complement(2025..2777)
                     /locus_tag="KPK67_003044"
     CDS             complement(2025..2777)
                     /locus_tag="KPK67_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2459974.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            complement(2777..2914)
                     /locus_tag="KPK67_003045"
     CDS             complement(2777..2914)
                     /locus_tag="KPK67_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2459975.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            complement(2954..3583)
                     /locus_tag="KPK67_003046"
     CDS             complement(2954..3583)
                     /locus_tag="KPK67_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2459976.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            complement(3544..4107)
                     /locus_tag="KPK67_003047"
     CDS             complement(3544..4107)
                     /locus_tag="KPK67_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459977.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFIKLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            complement(4117..4341)
                     /locus_tag="KPK67_003048"
     CDS             complement(4117..4341)
                     /locus_tag="KPK67_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459978.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            complement(4346..5437)
                     /locus_tag="KPK67_003049"
     CDS             complement(4346..5437)
                     /locus_tag="KPK67_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2459979.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSAAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVKEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            complement(5472..5930)
                     /locus_tag="KPK67_003050"
     CDS             complement(5472..5930)
                     /locus_tag="KPK67_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459980.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            complement(5923..7008)
                     /locus_tag="KPK67_003051"
     CDS             complement(5923..7008)
                     /locus_tag="KPK67_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG2459981.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            complement(7035..7220)
                     /locus_tag="KPK67_003052"
     CDS             complement(7035..7220)
                     /locus_tag="KPK67_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459982.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            complement(7382..7714)
                     /locus_tag="KPK67_003053"
     CDS             complement(7382..7714)
                     /locus_tag="KPK67_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBG2459983.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
BASE COUNT         2549 a         1359 c          854 g         2982 t
ORIGIN      
        1 ttattatata tatcaaatat tctttatatt ataatcatgt tgaataataa catatgatta
       61 atggaattac aatattttat aacacaaaat taactaatat aacaaattac catcagtcta
      121 tttattcaaa cttctaacta tataatatac atttatttat attaataatg caaacgatac
      181 cattatttgt aattaagaat tttggaatta ttgattacaa agtttttaca ttctcatctc
      241 acataagtaa tatttttatc aaaaaattaa tattattaaa ctttctaaat tacttatctc
      301 ttgatataat agtatacaaa acaaaccata tttcccactg aaactttgtt caacactagc
      361 cgtgtcaacg ttttcacagg aaatttgagt atataattta ttggaggttg atatgtacaa
      421 attaatcgca ctagacattg atggaacaat attaaataca caaaaaagga ttactcctga
      481 agtctttgag tccattcagg aggcaaaaag ggctggggca aaagtagtta taacaacagg
      541 cagacctctt cctggtgtaa aagaacttct aaatcaacta aatttaacag atgaaggtga
      601 ttatgttata tgctttaatg gagcaattat tcaagaagtt aaaagtgaga aaatcataca
      661 tgatgtagaa atgagtttag atgattttga ttttatatat aataacgttt gtaaaaaata
      721 taagactaaa atacacataa atacaatgac aaatttaatt acaccaaatg aaacgcctgg
      781 caaatataca cttcatgaag cgaaattaaa taatatagaa gtcaaatatg tacaaaaaga
      841 taaaattgat gaatctatta aaatctgtaa aattatgata gttgatgaac cagaaagatt
      901 ggaagaaatc atacagcaac tacctaaaaa tttattcaat aaatatacta tagtaaggtc
      961 tgctcctttt tatctagaat ttttaggtaa aactactaat aaaggaactg ctcttaaaac
     1021 attatgtacg aatctaaata tacctataga aaatgctatt gctgttggcg atgaagaaaa
     1081 tgaccaacat atgataaagt atgcaggtct tggtgtggct atggggaatg cacgtaatag
     1141 tataaaggaa attgcagatt atgttactga tactaataat gaaaatggag ttgcaaaggt
     1201 tataaataaa tatatactta ataaggctat ctaatcatct aaaaaaaact aaaaatatac
     1261 ctttagattt aaatactttt cacatcccta ctctatacta atttatatag tatggatatt
     1321 acctatcatt tttatctaaa ggtcattttt agtttttcat atatgtatat ctatgatagt
     1381 accttttgtt tgagtttaaa tattttatat gattctacta ttgcctcatt aatatctaaa
     1441 caatatatat cttcatcatc agataatccc acttcgtttg tccaagcatc aaaatcactc
     1501 ttcgtccaga agaaattcat tatatttcag caactagcag cccaatttag atgcttatca
     1561 agatttatat gaagcactct taagtttgga ttattcacat actcaatctt ttcatttttt
     1621 ataattactt ctatttcctt acctgtagca ctacaaatag agtttatttt tatatcttgg
     1681 ttgaatgtgc aagaggttcc tatagcatct atagcacaca ttgcactgaa acttctatta
     1741 tctctcaaag ttactttatg atttgttggc aatgctgata caggatatat aaaatttata
     1801 ctattcccat caacaaccat tatattttta taaataaaat attgtagtgt cctttctata
     1861 tattcttttt ctatattcaa ctttttactt acatattcag tcacttcttg aagtgtaaca
     1921 ctagaacctt tatctattat catatccata atagagattc ttattttctg ttgtgtatta
     1981 tccaaactat tgtatttttc tatttgactc atgtctactc ctctttaaac ttcataacac
     2041 tttgataaag tatcagttaa tttgtttttg tactcttcat atttttggct tcctctaatt
     2101 ctaggtcttg gaatatctat atttatatca tcaataattc ttcctttatc tctcgacatt
     2161 actataactc tatcactcat atatacagct tcatcaacat catgagttac cattaatgca
     2221 gttattttct tctttatcca tattcgttct agctcttcct gtaaattttg tctcatttga
     2281 aaatctacag ctcctaatgg ttcatccatt agtaatacct ctggattaca tgctaaagct
     2341 cttatcatgg ccactctttg tttcattcct ccagataatt gatgcggaaa cagtttttct
     2401 tttcctttca agtctgacat aaccaaaagt tcttctagca tttcttctct tttttctttt
     2461 ggtattttct gctgtttcat aggaaataga acattacctc ttatagtttt ccaaggaaat
     2521 aaagcataat tttgaaatat aaaagctcta tctatgcccg gttttttaac tcttttatca
     2581 tttataaaaa catctccacg ttctgatttt tgaaacccac ctattatagt aagtaaagtt
     2641 gtttttccac aacctgatgg tcctaaaaga cagacaaatt gtccatcatc tatatccata
     2701 cttatatctt ctaaaacctt tgtatgtact ctattattta caaatgtctt atttatatct
     2761 cttacactta acttcattaa tctgcaaacc tccatctaca aaggatttta tttataaact
     2821 ttaataccct atctattata aatcctatta aagctgcaaa tatcataagc ccaattaata
     2881 catctgtatg cattaatgtt tgagcttgta ccattgagta accgagaccg gcactcgtag
     2941 ctataaactc ggctcagata acagacatcc atcctgtact tatagcaatc cttgaaccag
     3001 ttagtatatc tggaagagat gcgggaacaa ttacatttgt aaatatcaca attggcgatg
     3061 ctcccatact tctagctgca ttataataat cctttgatat tgctcttact ccttgaatag
     3121 tatttaaaat tatagggaaa acccctgaaa aagctattaa aaatattgtt ggaccatctc
     3181 ctataccaaa ccatactata gtaagaggaa cccatgccat tattggaact tgccttacag
     3241 aatctactac tggagataaa accctttcaa aagttttaga aaatcccata ataaatccta
     3301 ttggaagccc aaacaataat gcatacataa aacctttaag tactctctgc aatgttataa
     3361 aaagattagt gactgtctct gcatctgtca cacagaaaaa gaaacctttt attacatcta
     3421 ctggcattgg aagtaacaaa gaactcccta tatcttttgc tgcaaaatgc cagactaaaa
     3481 atataataac taatagtatt attttatata aaatatccat actaccatca gtttttttct
     3541 tagttaatct atcgctttta tctttagaaa ttttaatttc caattgctta tttcctccat
     3601 atctatattt cttatattcc tgtactcttt taatgctttc ttaaatgtag ctttattctt
     3661 taaagcatca acacttttat tgtaaagttt tataaaatca ctatatgctt tacttttttt
     3721 aaactcttta cttacaacta atacataggt atcataatct cctaatttag ttgtagattt
     3781 tcttttaccc tctaacccaa ttgactttat gcaatctatt acaacaccat ctactaaatt
     3841 actttctaat ccatatgcta gtgcagtccc taataaggga gctacttcca cattcttgta
     3901 gtatttttta accaattctg actggtaatc tctattttga atgactccta tcttttcagg
     3961 attattatca cttaatagaa atatatcaga attttgaact acagtaccta caatttcaaa
     4021 tccactattt acatttacat attgttttgc agcatcttta catatgattg ctatatctat
     4081 ttcctctgaa ctcagagccc actgcattgt acttgctcag caatcattaa ttgtaaaaat
     4141 atccattata tcacttactt ttatattttc aaagtagtct ctatttacca tataatttat
     4201 tacaaaacca gaggtatcat cagatacccc tattcttaat tttcctttta tttcttcttt
     4261 atcttcaaaa ttaaaaaata ttataaatat acttcccaaa ataatgataa aaagtattat
     4321 tatatttttc atattctcca tttatttata atacctttgc tttccagtct tcataactca
     4381 taccttcagg aaactctggg ttaacttttt cttttataaa tttgtcaaaa tcatcagctc
     4441 cacaagagtc caataagctt gtatcaatcg tatcttcata tttaggtata tcatctaaag
     4501 cctttaaatc tttatacatt tgtaaattat ttttatattc ttcacctgta actttccaag
     4561 taagagttct accttctcca acagtcttct tatagatagt tctaagtgca acttcttctt
     4621 ttacattata atattttgca aatattttag ctgcttcagc tggatttgta tatatatatt
     4681 ctattgcttt tgtatgtgct agaaccaatt tttttgctaa atctggatgt tcttttataa
     4741 agtttttgtt taatgagaat gaacaacagt tatattcttt gccattagct ttttctttat
     4801 attgagtaga tgctattatt tttcctgcac cgtcattttc agctgcagaa ccccaagggt
     4861 cacaagatgt aaaagcttga attttcccag ttttaagagc taaatattta tctttgtctg
     4921 aatccatatt tacaacttca tattttgatg ggtcagttgg tagacctgtt tctggaccat
     4981 aatcacttgt ccataagaaa tcactcatat ctgttgcaat ttttttacct attaaatctt
     5041 taggttcttt tatatctttt gaaactatta aatattcaga accaccagta tggttgttag
     5101 ctgctattgt tattggtgaa cccttaggta ttgctccaac taacccctta gttcctatgt
     5161 aacctgcatc catttttcct gctgccatag cttcaggaac tttaccattt ccaacagttt
     5221 ttacattaag acctaaatct ttatatattc ctgccgattc tgctactggt cccgcagtca
     5281 tatggtcaca attataataa cctactgtta ttgtataatc atcttttcca gtttttgctt
     5341 cttctttttc ttttggttta ctactacatc ctgttaatat agatgtaccc attacaccaa
     5401 ataagattaa tgcttgaatc ttcttagata atttcatata aatttcccac ctttatgtat
     5461 tatttttatt tttagcaaca tttgtccata tcttcgccaa ataaatcaac aaatttttta
     5521 ccctctactc tgtaaatagc atatcctaca ttaccatttt tagaccctga tacaattact
     5581 tctattggtg ctttatcatc aatattttta aaggttatag tttgattctc tataggtgct
     5641 ggtattggct ttgttatata gtggctattt ttatcagaaa ctacgactac catttcattt
     5701 gaattattgc tttttttata tataactact aaatcttttt tcccatcatt atttaagtct
     5761 tcttcagcac aagttattat gtcttcttta taattttttc taaaatattc taatgtttct
     5821 ttattcacat ttggaataga agcatcttct ttgatgttat catcaactat ttttgataaa
     5881 atgattaaca caactactat acaggatatt ataactatct ttttaagcat gatttcctat
     5941 gacctctttc atctttggta ttattgattt aaatgcaaat aaaataatta aaatagtaca
     6001 tacatactca aaactatcag gtatatttaa cattagttta tttgccaact gtattgaaga
     6061 atttgttgca tctaaattta ctgagttatt attcactaaa agtaaatttg ttatatatcc
     6121 aattattatt gaagacagtg tcaaagttac agaatatata ataacagttc tttttccaat
     6181 aagtttaaac atacttataa gttctggtag atttgtagct gctccagcta ttagaaatgt
     6241 tatcgctata ccaggagatg ctccacttgc aacaagggct gctataaacg gtatatgtcc
     6301 tacagcacat acatacataa cacatgcaag tattgctata cttccaagag atagcaaacc
     6361 tggatttcct aaatattttt gaattatact atttggaaaa atagttgtta taaatcctgc
     6421 aaaaaccata cccattacta catatttact aatagtaagg gctaattcac caaaagacca
     6481 tttaagacca tcaatcatat tttccataaa acttcttttt tcttcttcta attctattac
     6541 ttcttcctct gaatcattat gaagtgataa ttcttctttt ccaaaacaat ttcctaaaaa
     6601 tcctactaaa aatggtacta aaaaaccagc tattagataa atgattgtaa ctttaggacc
     6661 taaaagacct agacaaagaa ctacagctat tggatttatt attggagttg atgtcataaa
     6721 agcaagtact ggtcctaaat atgctcctga ataatacata cttattccaa gtggtattac
     6781 tccacaacta catattggta aaaacattcc tgagatagta cttttaatta aagaagaaaa
     6841 tttcttattt ccaagttgtt tttgaaacct tcttggagtt aaaacattgt gaagtacacc
     6901 tgccaatata aaacttatca taagccatcc tgatgctcca ttcagtgtaa ctatagaagc
     6961 tactatagta tctttaactc catttaaaac aatttctaat atattcattt ttgatactcc
     7021 taatttttaa acttttaatt attgtttata gcttctacta ttgccctttc aattgtatct
     7081 ttatttaatc tatcatattt ctttttctga tttattatta aagttccttt tgttataatc
     7141 ccatattttt ttatatacga aaaatctttc cctgcttgat ataattttat ttccaattta
     7201 tcagcatact tttctgacat tttttttata aacactccat gttcatcaca agatggtcaa
     7261 gtgtttataa attcaactaa aattttagac atttgttaat cccctacttt aaacaattta
     7321 tcttttactt aaaataatcc acagaaaata aatttttcta tggattgtat cctatactaa
     7381 tttataacgt tataacttgg tccacacctg cactgcctaa acaagcatat aggtttggga
     7441 aacatccaat tgtagctcca tcaaccaaat tattctctat acctctttct atagcacatt
     7501 ggtcacaagc cattattatc atatcagttt tttcatgtaa tttacttagt ctctcgccta
     7561 caggattacc tttagataaa aataaggtat tatccataaa gaaaaacatc cctagaacgt
     7621 ctactccatg agttccttgt tcaagttgtg gtattatcat tttgtctaga attttatgtg
     7681 tattagtact actaaaaata taagctactt tcatttttat tccccctata ctttttatat
     7741 ttta
//
LOCUS       DAEMHW010000173         7730 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEMHW010000173 DAEMHW010000000
VERSION     DAEMHW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.173"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            953..2311
                     /locus_tag="KPK67_003054"
     CDS             953..2311
                     /locus_tag="KPK67_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG2459984.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     ASGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2418..3212
                     /locus_tag="KPK67_003055"
     CDS             2418..3212
                     /locus_tag="KPK67_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG2459985.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3205..5511
                     /locus_tag="KPK67_003056"
     CDS             3205..5511
                     /locus_tag="KPK67_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG2459986.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPVFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5650..6927
                     /locus_tag="KPK67_003057"
     CDS             5650..6927
                     /locus_tag="KPK67_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2459987.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7110..>7730
                     /gene="yqeC"
                     /locus_tag="KPK67_003058"
     CDS             7110..>7730
                     /gene="yqeC"
                     /locus_tag="KPK67_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBG2459988.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGVLDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKY"
BASE COUNT         3029 a          840 c         1444 g         2417 t
ORIGIN      
        1 cttgtatatt ttatgttgca gatacaaata tagtaaagga gagtacattg gtacaactga
       61 gatacatagc ttattaagtt tttctgtctt atgagaatta tattataaaa tattcataaa
      121 tattttgagt ttaaattaaa taattatttt atgcttagat taattgatat ttaattaatt
      181 taataacaaa aacagtgaaa gagtaaataa tattatttac agggggtaaa gcaaagtatg
      241 ttaaataaat tgtacttttg gccccgtaca tagagaaatc tatgtaggac acaactttaa
      301 aaccagtaaa tcctaaagcc ttgcaactac aataaaagat agaaagaaac ttttatgaat
      361 gtggtgaaaa ccagaaacaa tagtgaggat ggtatatggt gagagcctaa gtacttaaaa
      421 atggacattt ggtcgccaag cctagaaata ggaaggtcaa acgactatct cgagagagag
      481 tacactataa gcgattgata gtggaaacgg gttgctctta gcaggtaatg ctgaagatga
      541 agaaatagtc tacgctcata tgaaagtatg aggtcactgg atttgaagtg acatctataa
      601 agtagcgttt ataggtaaat gtttaaaact tgaaacatgt aaatacttat gaaaacttat
      661 aacttataat gattaagatt gaaagattta aattgaatta aatttactat aaaaaattaa
      721 aaattcatag ctataatact cttatatttg taaagcatgg tatataatgt atacaaaatt
      781 aaagaatacg tttatttgtt aataattaaa tttattgcat ctgtttttta agactaaaaa
      841 cttaaatata tattggagta tagttgatat ttatgaacat cattaattta atatattaga
      901 tttttaagta tttatataat tcaataagtt ttatgtaacg ggagttttta ttatgaaagc
      961 aaaaagtaca tcaaaatttg aattagacgg aataccacca ctaaaggaag caataccttt
     1021 agggttacaa cacttaatgg caatggtaat aggtagttgt ttaccagcga tattgattgc
     1081 aaatgcatct ggacttaatc ataagatgtc aacattattg attcaagccg cattagtttt
     1141 tgcaggatta tcaactttat tacaattata tccaatacct ttgtttaaag gttttaaatt
     1201 aggaggagga ataccagtta taatgggagc tacagctatg tttataggag ctggggtatc
     1261 tgttgcaagt aagtatggac taccagtatt atttggctct caaataatag ctggtatagt
     1321 aataattata attggttttg gtcttaaaaa aatacgattt atatttactc cagctgtatc
     1381 aggaacaata gttgcatgta tgggtcttgg actttttcca atagctataa aaaacttagc
     1441 tgggggtatg ggcaatgaga cttttgggaa tccaattaat tttatagttg gaatatctgt
     1501 atctcttatg atattaggat taaataaata tggaaaagga ttatttaaag atgcatcaat
     1561 attagttagc ataatttttg gatacatttt atcattaatt ttgggtatag taaatttttc
     1621 ttcaattcag gagtttactt tggttgcact tccaaaacca cttgcttttg ggctagatat
     1681 aagacttgaa gttgttgtaa tgttttcaat aatatattta gttgagatag ctgatataat
     1741 gggtgcttgt acactttctg cggtaggagg gcttaataga caagtaacag atgaagaact
     1801 ttcttctgct gttttaggaa atggagtact ctctacaata ggtgcattat tttctgcaac
     1861 tccaatgggg atgtttggac aaaattctgc aatcgtttcc aatacaaagg ttgtaagtaa
     1921 atttgtacta gctattggag gaattggatt atttttggca ggaatatctc ctttattagc
     1981 taatttaata agaacaattc ctccctgtgt ggttggagga gcaacactcg ttatattttc
     2041 aactcttaca acatctggtt taagacttgt tagtatggat ggatttaatc aagaaaatag
     2101 catgatatta ggtctttcta tggcttctgg tattggattc atggtagctc ctcaagttct
     2161 tgaaaaattt ccaaaattta tagagacatt acttgcagat tcaagtgtag tttcaggagc
     2221 aatggtagcc ataataattc aagctttata tatggtaaaa tttcctggaa ataaaagtga
     2281 taaggttgag gataaaaata aaagtttata atgtaaaggt ttaatatatg tgaattaaat
     2341 atactacaat tataaatata aataaaagat ttattaaaaa cttttgtgtt ttaataagtt
     2401 aatgatgggg ggataagatg gttactataa aggaatattc agttccgcag agtttagaag
     2461 aagcatataa aattttaatt tctaaaaaaa ataatgttat tttaggtgga tgtggattta
     2521 taaaattaag taataaaaat ataggaacag cgatagattt aaaagatata aatctaaatt
     2581 atataaaaga ggatgaaaaa aatattttga taggtgcaga tacttcactt agaagtttag
     2641 aaattgataa aactataaaa aattactgta gtggaatact ttcaagcgct gtttctaata
     2701 tagttggtgt tcaatttaga caaggggcta gaattggtgc aagtgtattt tcaaagtatg
     2761 gcttttctga cttaatacca gcacttttgg ttgttggagc gaaggtgaga ttatacaata
     2821 aaggaatact agagttaagt gagtttttgg atagtgaatt aagtagggat atattggtcg
     2881 aagtaatact accaaaaaaa gatgcaatag ctgtttttga ctcaataaga aaatgcacag
     2941 gagatttttc tgtattaaat tcagcaatgc taaaagaaaa tgatacttac aaaatagcaa
     3001 ttggggcaag acctcaaaaa gctagattag cacttgaagc gagtaatata ttaaataaag
     3061 aaaaagatat agataaggca tctattgttg ctagtaagga acttacttat ggaagtaata
     3121 tgagagctag taaagagtac agaaaggata tggcaagtgc attagttaaa aggatgtata
     3181 atgctattga ggagggaatg tataatgatt aataagggga taagaaagat agatagtaca
     3241 gcaattgtca caggaaaacc tctatatact gaagacctca ttatgcataa agatgttttg
     3301 acattaaaac tacttagaag tccacatgct catgctaaga taaagaatat agatacatca
     3361 aaagccgaaa aaattccagg ggttgtgggt atttatacgt ataaagatgt accacaggtc
     3421 agatattgtg ttccaggtca aacagaacca gaagcatcac cttatgatag agttttactt
     3481 gaagatacag ttagatttat tggagatgaa gtagctttag tggctgctga agatgagaaa
     3541 acagcactta aagccttaaa aatgataaag gtagattatg aggttttaaa gcctgtgttt
     3601 gatgcaagag aagcagaagg aaatgaagtt gttatacatc cagaaaaaga catagtagag
     3661 ccataccctt ttggattgga tgcaaaaaat aatatagtat caaaagaatt ttttgaattt
     3721 ggagatgttg aagctaagct taaagaaagt gattttgtgg ttgaatcttc atacaaaacc
     3781 caaagtcaat cgcattgcat gatggaaact cataggtcat attcatataa agatataaat
     3841 gatagaattg ttgtagtttc atcattacaa tcaccacaca atgtaaagag aatacttaga
     3901 aaagtattgg gtataccatc ttcaaaaata agagtaatta aacctagaat aggtggtgga
     3961 tttggaggaa agaatatagc agcaacagat atatttactg catttgttac tatgaaaact
     4021 ggtagaccag caaaatttgt atttacaaga agtgaaactt ttgtaggttc taatagtaga
     4081 catgaaatgt tttttgatgt taaagttggt gcagataaag aaggaaattt aaaagcaata
     4141 gagctaaagt cattaaacaa tacaggtgct tatggtggga atggtgtctc tgtttcttcc
     4201 gaatctggac acaatacctt accaatatat aatgatattg atgctgttag atttaatgct
     4261 agaacagtat atactaatag gttaccagca ggagcactta gagggtatgg tgctacacag
     4321 ggaatatttg caatagatag tgctattgat gaattggcac ataagattgg aatggaccca
     4381 cttgaattta gaattaaaaa tataatagat aggtatacca atggaaaagc agctgaagac
     4441 gaaatattaa gttgtgagct gagacaatgt atagaaaagg gaaagaaatt aattaattgg
     4501 gatgataaat atccatgtaa agatatagga aacaacaaag taagagcagt aggtatggca
     4561 gtagcaaatc atggttctgg tataccgcgt gtagatatgg caacagtcac tataagaatg
     4621 gatgaagatg gcacatataa actattatct ggtcttgctg acttaggtca aggggctgat
     4681 acaattcaag ctcaaattgc tgcggaagct ttaaatacta ctgtagatag aatttctatt
     4741 tatacaggtg attcagattt atgtccctat gatacaggag catttgcttc ttgtacaaca
     4801 acggttacag gaaatgcaac aataaagaca gcaaataaat taaaagatat attactttca
     4861 gttgcagagt caaagctagg atttaaaaaa gaagatttag agttgggaaa agacagggtt
     4921 tttgttaaaa atgataatag caagtttgtt ctattagagg aattgggtag agaatctgtt
     4981 ggaggaatag gatttgctag tgatgatgta ccagtagttt tggaagcaac agaatctttt
     5041 ggcatgttga aaaaaataaa accatttata gcaggatttg cagagataga attagataaa
     5101 aatacaggcg aatataaagt cataaatttt gcatgtgtac ctgattgtgg aacagttata
     5161 aatccaactt tagctagaat acaagtagaa ggtggtgtga tgatgggtat agggtttgca
     5221 atgtatgaag accctgtatt tgataaagat gggagattat taacagatag tttcctacag
     5281 tataaagttc ctacaaaaaa agaagttgga aatatattgg tagattttgc tgagaatttc
     5341 gacccaggag gtccttatgg tgctaaatca ttaggagaaa tagttataca tacaccagca
     5401 ccagctatag caaatgcaat atttaatgct acaggagttc gtttaagaga gcttcctatg
     5461 acttttgaaa aagtttatat gggtatgaaa aataataata aacaattata ataaataata
     5521 aggtgtataa aagcaagtaa atttaaatta ttttgtaaaa taaagtaatt aaatggacat
     5581 tatatatggt gttttgtaaa aataccgtat ataatgtcta gtagagtata ttctaaagga
     5641 ggtgagttca tgtcaaacag aataaaaaat atacactatg gaactcagag acaacaatta
     5701 aaaaacttaa ctaaaaaaga atatcaagct ctacgagaga tgtgcttctt agctaagaac
     5761 ttatataatg ttggtacata taatgtaaga cagcattatt tcaatcaagg agaacatctt
     5821 aactatgaag ctaattatca tgtatgcaaa gaaaatgaga actataagct tctcaatagt
     5881 aatatagcgc aacaaatatt aaaagaagta gatagttcgt ttaggtcatt ttttgggtta
     5941 attaaattag ccaaagaagg aaaatatgac tttagaggga taaaatcacc taaatattta
     6001 gataaagaag ggttttttag cataataata ggtcaaataa gaatcaaaga agatgggatt
     6061 ttaaatgtac ctatgtctcc agcatttaaa aaactatatg ggaaagtaag tataaaagtt
     6121 ccacaaaata tactagagaa aaaaattaaa gaaataagaa taatacctaa acataaagct
     6181 aggttctttg aaattcagta ttgttatgaa ataccaaaat cagaggaagt ttcaaatcaa
     6241 aaaaatgcac tagctataga cttgggttta gaaaatttat gtacatgtgt taccaattta
     6301 ggtgatagtt ttatcataga tggaaaaagg ctaaaatctg tgaatcagtg ggcaaacaaa
     6361 cagaatgata aaatagaagc cttaaaatta aaaaagaata taactgcaat aacaagaaaa
     6421 caatttaaag tttggaataa gagaaataat caagttaaag actatgtaaa taaaacctgt
     6481 ttttatataa ttgattattg tgtaaaaaat aatattggaa atttaattgt aggatataat
     6541 gctaagttag aaaaaagtga taatatggag agaaaaatat atagaaattt tataagaatc
     6601 ccatttggag agataaaagg aaaattagaa tatctgtgta aaataaaaaa tatcaatttt
     6661 attaggcaag aagaaagcta tactagtaaa tcagattttt ttgctgatga ttttctacct
     6721 gatatcaatg tggacaatgt taaaaaatat gactttaaag gggaaagaat aacacgtgga
     6781 cagtacaaat ccagtgttgg aatcattata aatgcagatg taaatgcgag tcttaatata
     6841 ctaaaaaaat cacaagtagt taatttatct aatttacaga ataatcctca aatattaaag
     6901 caaccacaga gagtaagaat atcttaatta tatttaaaag ataaactgat agcgtaagct
     6961 tacagcttag cggcgaattg gatccgcctt gttgtttaca acagaatcta tttactttcg
     7021 agtttatgga atttcaaaaa tcacttttac taaaaaggtc taactataat aattaagaga
     7081 ttagaaaact ggttataaga ggtgaaagtg tggaactatc aaatataata gaaaagaatg
     7141 aaataataac tgtagttgga gcaggaggaa aaacttcgtt tataaactat tttgcaaatt
     7201 tttatagaga taaattgaaa gttctcctta caacaacaac taaaatatat gtaccaaacg
     7261 attatgacaa tataattatt acaatagatg gaactgttat tccatctatt tgtcatggaa
     7321 taacagtctg tggcagttat ataaataatg aaaataaatt agtaagtatt gattcctcta
     7381 ttttggatga aattgtagac caatttgatt tagttttaat agagggagat ggctcaaaga
     7441 gaaaaaaact taaaggttgg aatgctaaag aaccagtagt atatcataaa accacaaaaa
     7501 ccataggggt attagatata acatcatttg gaatgaatat taatgaagag aatatacata
     7561 gagtagaaat atttaaaaaa atagcaaatt tagatacaag tagtattaat agtagttcta
     7621 cagtaagtat tgaaaactta aaaaatatag tattaaatcc aaatggatta tttaaaaatt
     7681 attctggaaa aagagtatta tttattaata aagttgaaaa tgaaaaatat
//
LOCUS       DAEMHW010000174         7607 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEMHW010000174 DAEMHW010000000
VERSION     DAEMHW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.174"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..419)
                     /locus_tag="KPK67_003059"
     CDS             complement(<1..419)
                     /locus_tag="KPK67_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2459989.1"
                     /translation="MYSKIAIKNVRKSFKDYSIYFLTLTLVICIFYSFNSMESQKALM
                     EMSTSKKPDLEMFKNMISYISVFVSAILCTLILYINNLLIKKRNKKLGIYMTLGMGKI
                     EMSKILVFETIIVGIVSLVSGLILGLLVSQGLLILLL"
     gene            complement(409..883)
                     /locus_tag="KPK67_003060"
                     /pseudo
     CDS             complement(409..883)
                     /locus_tag="KPK67_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521021.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1579..2484)
                     /locus_tag="KPK67_003061"
     CDS             complement(1579..2484)
                     /locus_tag="KPK67_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBG2459990.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNICQLTK"
     gene            complement(2564..3466)
                     /locus_tag="KPK67_003062"
     CDS             complement(2564..3466)
                     /locus_tag="KPK67_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG2459991.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(3516..4676)
                     /locus_tag="KPK67_003063"
     CDS             complement(3516..4676)
                     /locus_tag="KPK67_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG2459992.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(4831..5136)
                     /locus_tag="KPK67_003064"
     CDS             complement(4831..5136)
                     /locus_tag="KPK67_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG2459993.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(5234..6208)
                     /locus_tag="KPK67_003065"
     CDS             complement(5234..6208)
                     /locus_tag="KPK67_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG2459994.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            6790..7566
                     /locus_tag="KPK67_003066"
     CDS             6790..7566
                     /locus_tag="KPK67_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG2459995.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRADDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
BASE COUNT         2530 a         1309 c          834 g         2934 t
ORIGIN      
        1 tttaataata gtattaataa cccttgagat actaataatc caagtattag cccacttacc
       61 aaagagacta ttccaactat tatagtttca aaaactaata ttttcgacat ttcaattttt
      121 cccattccta aagtcatata aattccaagt ttcttatttc tttttttgat taataagtta
      181 ttaatataaa gaattaaagt acataaaata gctgatacga acacagatat atatgaaatc
      241 atattcttaa acatttctaa atcaggcttt tttgaagtac tcatttccat taaagctttt
      301 tgagattcca tagagttaaa actatagaaa atacaaatta ctaatgttaa agttaaaaaa
      361 tatatactat aatccttaaa gctttttcta acatttttaa tcgctatttt agaatacatt
      421 gttatcatct cctctgatta tagctatttc ttcaattatt ctattaaaag actcttttat
      481 tgaatcagtt cgtcttgcta actctataaa tatttttctg tattttataa ataagtttct
      541 atgagtataa ctagctataa aagcatcatt agttagcatt aaaattgttg tttttaaatc
      601 ttgatttaaa ctttcaaaac tctcaagtaa taatcttgat gcttttgaat caaatttata
      661 ttgttttccc tttaattgtt aatgtcaatg ctatattttc ataagctatt aaagtatata
      721 aaagattaaa attttggaat ataaatccta attcattttg tctaaattta tctaagtttt
      781 tgaattttaa tcttgtaata tcattactat ctattgcttt tgtaatatta tctttgtttt
      841 cataatactt ttctattttt tcaacactta atacattttt catgccttat ttctccttta
      901 aatttcttat atatctattg taatttttaa tttaattttt ttaaattgat ttacatttca
      961 ttaatatttc acttttgtag taattgtata ttgctaccta taattactaa tataaaatgt
     1021 tcaagaccaa cagattttct tttgtgcttt actagaatcg taaaatttac ccaattctag
     1081 aaaaacacaa aaaatggaag gcaatgcctt ccctctaaaa tttattttat tctaattgta
     1141 tttttctata tgttctttca aaatcattgg tattaaatca ttcttactat agtgctttgt
     1201 atcacaaaat tattgccaat cttgatatgt aaattcattt atctttatac ttttaagctt
     1261 gaagtctaag tgtttatcat ttattagatt aattattaca tgatttgtag tagtttatat
     1321 gtggctctta ttgttaactt atacttatct aaaaatgttt gtaaaactcc aagattattt
     1381 ttaatagata aaggctattg ctttaatatt tgtgctaact tttaaactaa tatgctatct
     1441 tagtattatt taatttttat taacataatt gtaatttatt gttgaccact tttcaaagtt
     1501 gatttaacat atataaaaag agtgtttaaa atgattataa aaatcagttt aaacactctt
     1561 tttttgctca tatatatctt acttagtaag ttgacatata tttaattcat tcatcaattt
     1621 tttaatttca catacttgtt tttcatttgg ttttacaaat ggagcagtca tataatcact
     1681 tatttgtaac cctctgatat tcatagcttt tttcattgct ggtataaatg gattggttat
     1741 taagtaaaaa tccattgctt tattaatata tttttgaatt tctataacct tctccatatc
     1801 tctattattg taagcttcag cccatttgga gcaaacttct ggaattaaat ttgataaccc
     1861 ccctatacaa cctgctcccc cagacattag attatgtata aagttttcat cataaccact
     1921 ataaacctta aattctggga actctatttt catagtattt attaaatctc tggtatgtcc
     1981 catcaaagtt actgtatcct tatatccaac tatgttttta tgttttcttg caagtcttaa
     2041 tgtaagttct ggacttaaat cataaccagt tctatctgga taattgtaca aatatatttc
     2101 agcctctgta ttcttagcta tttcactata aaataactca atactatcat catcaagaga
     2161 gaaataataa gggcttatta tcataacacc atctgctcca ctattatgtg catagtttga
     2221 aagttctata gattcattta tacacatacg acttgtacct ataaacactt ttactctctt
     2281 atttacatga ctagttacta aatctataag ttctttttgc atatccatag acatattaaa
     2341 gaactctcca gtacttccca ttatgactag tccatctaca cctccatcta taagatggtc
     2401 atacactttt ttattacctt ctttatctaa atttccttct tcatcaaata ttgtaacaac
     2461 tggtgtcaaa aattctgctt tcataattta aactcctaaa atttatttta atatgttcat
     2521 taatacatca gatttagctt taatattaat tatattaaaa catttactta atgtaatttt
     2581 ttaaaattcc aatactctca atctcacatt caactacatc tccactactc atatatcgag
     2641 gaggtgaaaa tcccatccct actccagaag gtgttccagt agctataatg tctccagcct
     2701 ctaaagtcat accttttgaa agttctgaaa ctatgtctga aacgtctgat atgaataatc
     2761 ctgtattaga actttgtctt tcttcaccat ttaccctact acatacttta agttttatgg
     2821 gaaaaggaac tgcactttta tgtaaaatac atggacctaa agaggtaaat gtatctaatc
     2881 cttttcctct aaaccattga acatgttctt tttgcaactt tctagcagaa atatcattta
     2941 taacagtgta accaaagata taatcttcta cctcttcttt tctaatatta acacctgttt
     3001 tacctattac tactccaagt tcaacttcat agtctagttg attatttatc tcaaaatgac
     3061 catctatctc atcttctgga cctattgcct ttgtcacacg ttttgtaaaa taaaccgttt
     3121 tttgtggttc ttcaaaacta tcatcaaagt gagtttgagt ttcttcaaga tggtctttat
     3181 agttcactcc cacacataag atatcatgta ttggtttttc tataggaaca tgtacagtac
     3241 atctctctct tttaaaaaca tcataattat agaatgtact ttcatttata atagttttca
     3301 acttaataat atcttcacta gatacatttt gaattaattc atttaaatcc ttaaaatttc
     3361 tactgagatt taaagagtta acatctataa taaataattc atctttagaa aaaactccag
     3421 ttcttttaca ttcattttct ccttcagtaa aacttacaaa cttcataatc tcacccttta
     3481 ttaaaattct atgtatttat tttcttactt aaagattata catagttgca tactctagct
     3541 attgccattt cagtatatcc aagggactct gcttttgatt gttttccatt tgctacatct
     3601 acaaccatat ttaacaaatc atctgttaat tcttccattg tttgtggacc atagataact
     3661 ggactagcat caaaatccat attatcttcc atatttgcaa aagttatttt atttccagtt
     3721 atttttatta ctggtgctat tggatttcct gatggtgttc ctcttcctgt actaaatacc
     3781 actatttgtg ctcctcctgc taccatacca gcaacagatg aagcatcatt tccaggggta
     3841 tccattataa caagaccttt cttatcaatt tgtttggcat aatcatatac ttcacttatt
     3901 tgtctatgac cacctttatg tatacaacca agtgattttt cttctaaact tgttaaacca
     3961 ccagctatat ttccaggaga tggattacct tctctaactt cttctccaac caattttaat
     4021 gaattttcat atctatgtac tatatgtaat attttttctt ttacttcttc atttactgct
     4081 ctcctagcta gtatatgctc tgcacctata aattcagtag tttcacttaa aattgatgtt
     4141 gctcctaaat cgacaagttt atcacttaat tcacctatta atggatttgc tgctaatcca
     4201 cttgttgggt cagacccacc acattcagtt cctagtataa gttcagatat tggaaattct
     4261 tcttttctaa gcaaagaagc ttcttgtgcc atttctctag cataacgaac agctttttct
     4321 atagttttta aagttccatg ttcttcttgt attacaagag tttttattgg cttatttgtt
     4381 ctctctttta ttgcatcaac taccaaatca ttttggcaat tttcgcatcc caaagataca
     4441 actattacac catatacatt tggatttgct gcataaccag ccataacatc catagttaat
     4501 tgttggtctg aatgtacttg tgaacatcca ttctgattat taaaggtaac tgccccttga
     4561 acttgtgatg ctattattct agttgtatct gatgcacaca cactcgtagg caatattaat
     4621 atgtggtttc ttataccaac tcttccatct ggtcttttat atccataaaa tttcatactg
     4681 atttcctcct aaaatagtct atatactttt tataattttt atatattata atttttatta
     4741 cactcactaa tcaaacagct aaaatcctat ttaaaaacta tttgacacta aaagcaatga
     4801 atacaacttt tataactcta taaaattatt ttatatatta gtcataactt gtttatccaa
     4861 atcctctcta acacttctaa cattatgaat atgtacatgt tgaccacttt ttatttcctg
     4921 atttgcttct cctatatgtt caccatattt agtaacttta tcaccttggg ccatatcttt
     4981 tatagccaat ttatgataaa tagttatatc atctagtgct gtaatcgttt ttactgtttt
     5041 gtcttttaat ttaaaactaa tttcactacc tttagctatt gcttcaatag ctactgcaac
     5101 attatctttt tcatcaatta tcactgcatt tatcattatt cttgctctcc tttataaata
     5161 atataatttt attaatatcc taaaatctaa ttaaagaatt tattatttta ccaacaactc
     5221 actgtaatat aaattaagct gttattgtac catcctcatt ttgttttggg aacttaggac
     5281 atccatactt cttagcccac caactagtta taagaggtac taatattgca gtgacaacta
     5341 ctgcggctgc cacttgtgtt gtagctgttg caacatatgg ttgccatgat gggtcaacta
     5401 atgcaacagc agcaggtaca gcaattgcat ttccagcagt tgtagctaca gcccatcctg
     5461 catatcctgg acgttttcct ataaatttat cacagaatac tataaatata cctcctacaa
     5521 atacagatat taatccaagt aatactcctg ataaacctcc ttttagtata cttgcaagat
     5581 ttatgctact accaagtgca aaccctacaa aaggtacgag taaattacac cctggctcta
     5641 aaaactcttt catttttttg tcaaaatttc ctattatcat accaacaagt attggcacta
     5701 ttgcagccaa taatgacaac aatggtacat ttgcaagacc tgaagtccca agagctatta
     5761 atgtaaaaaa tggtccatca tttaaagtaa gtaaactcat agttgcacaa tctgtttcat
     5821 ctccatatga agtcatcaat gaaagatata tactaccatt actatttgta actgcactta
     5881 taacagccaa tgttgtaaga ccaaggacac catccattcc aaatagcttt cctataagta
     5941 tacctatcaa agcacctata gcaaatttag aaccaagaag tataccacct ctctttaaaa
     6001 cttttggcat ttctctaaat tgaagctgag ttcccaagca taccagttga accccaatta
     6061 tagttgcaga cccaacactt gaaaatactg ctgtcgtaaa cgaacctatt tgtacaatat
     6121 ctgggcaaaa ggtattaatg aaagctgcta taaacattgg cactatcatc atacctgcag
     6181 gaattttttt aataaaatcc attatcataa agttaatcct cctaaaacat gatatttaat
     6241 tactcttatt aagtttttag ctttaattct aacggccgtt tcggttcgta tttaagaact
     6301 aagtttttaa ttacgaatct ttgaattaat gatagcattt tcctatttat gagtcaataa
     6361 aaaaaataaa tttttgtcaa aattttttca aactttctga taataccttg tatattatca
     6421 aatgacttcc ttatatgcaa aaatacagcg tatttaaaat tatatagtat tcaaaactat
     6481 aaagttttta tacagtgcaa aaaatagata aaatataagt tcataatatt ttgatattta
     6541 catataaaaa taactattgc atatatttac ttaaattata taactaaatg tcacttactt
     6601 tctttaggta tattttggaa attagttatt ccttaatgta cagtctaaaa aaatatctat
     6661 ctgtatctaa atcaagagct tatttatata taaatttaca tatttatttt ctacattgta
     6721 aaatatcgta tagtgtgcta aaatcaacat attctaatac tttaaattaa taaaaagagg
     6781 gggaaattta tgacttcaga taatcatcgc ccaacagcta ggatattaga tattttagaa
     6841 gcattagcag aatccgaaaa tggatatact cttacagaaa tagcagatgt tatcaatgct
     6901 ccaaaaagta gcatctttcc tatagttcat acacttaatg ctagaaaata cattactatt
     6961 gataaaaaca catccaaata ttctataggg atacgttctt acacattagg gtcttcattt
     7021 tttgaaaaaa gaactatttt taactatata attgacgaaa tgactaatat agttaattcc
     7081 tgttctgaaa cttgtcagct tggtatactt gacaataatg aagttcttta tattggaaaa
     7141 gtagattctc cagaaccaat tagacttatt tcttatgttg gaaaaaaaat acctgctaat
     7201 tgtacaggtc ttggaaaagc tcttttatgt gattttgaca aggaaaaact aataagctta
     7261 tatccagatg gtctaaaagg ttttacagaa aaatcaatta cagattttga tgttttatac
     7321 gaccaacttc ttgaagttca aagaacacaa atagcttcag agtgtgagga atcaatggaa
     7381 cacctaaaat gtcttgccgt accaataaga aaaaatggat gtataagagc tgctgttagt
     7441 gtaacactgc ctttatttag agctgatgac gaaaaaatag aactgataaa aaaattactc
     7501 ttaaatgctc agaaaaatat tcaatcaatg atggaagaac gtaatgaaga cattgtaata
     7561 aattagatta atattctgta tattgaagat atcaaaacat aataatt
//
LOCUS       DAEMHW010000175         7581 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEMHW010000175 DAEMHW010000000
VERSION     DAEMHW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.175"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            21..404
                     /locus_tag="KPK67_003067"
     CDS             21..404
                     /locus_tag="KPK67_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG2459996.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            406..756
                     /locus_tag="KPK67_003068"
     CDS             406..756
                     /locus_tag="KPK67_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459997.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(857..1315)
                     /locus_tag="KPK67_003069"
     CDS             complement(857..1315)
                     /locus_tag="KPK67_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2459998.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLKEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(1360..2148)
                     /locus_tag="KPK67_003070"
     CDS             complement(1360..2148)
                     /locus_tag="KPK67_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2459999.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQGTDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(2287..2724)
                     /locus_tag="KPK67_003071"
     CDS             complement(2287..2724)
                     /locus_tag="KPK67_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG2460000.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            3544..4053
                     /locus_tag="KPK67_003072"
     CDS             3544..4053
                     /locus_tag="KPK67_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2460001.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            4457..4993
                     /locus_tag="KPK67_003073"
     CDS             4457..4993
                     /locus_tag="KPK67_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG2460002.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKR"
     gene            complement(5414..6214)
                     /locus_tag="KPK67_003074"
     CDS             complement(5414..6214)
                     /locus_tag="KPK67_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460003.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(6204..6956)
                     /locus_tag="KPK67_003075"
     CDS             complement(6204..6956)
                     /locus_tag="KPK67_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460004.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEAISISDKVAILSKRPATIKNIYDIELN
                     MDGTKTPLKTRKVPDFQKYFDILWKELDTHEA"
     gene            7126..>7581
                     /locus_tag="KPK67_003076"
     CDS             7126..>7581
                     /locus_tag="KPK67_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460005.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAF"
BASE COUNT         2706 a         1136 c          993 g         2746 t
ORIGIN      
        1 ataataagga gcgattaaac atgaatctat cgaagaaaat agaagataaa agaaaagcta
       61 aacagagagc tgaaaaagtt aagaaagcta aaattgctac agctggtgtg gtattaggag
      121 ctgttactgg tgcagtgagt ggagtacttt tggctcctaa atctggaaaa gaaacaagag
      181 aggatataaa agatgcttca caacaaattg ctgaaaagat aaatatgaaa actgtagatg
      241 taaaaggcaa agtatctgag aagctagagg ataaaaaagg aaactttata gaatcaaaga
      301 aaaaaataaa aaaatatata gataacaaaa agagtgttga aaattctagt gaggaagata
      361 aaataataga tatagttgaa ccagttagtg ttactgagga ataatatgga caatacaata
      421 acgattaaca taagtataat atatgtatta ttagctatac ttgcttgtat tgctttagtt
      481 tatttaatta ttgtacttgt taaactacat aagattttaa ataatgttgg aactatgtta
      541 aatgaaaata ctcaaaatat aaatacaact ttaaataatc ttcctaatgt tgtagaaact
      601 tttggagaag taggagaaaa tatgaaagat gtaacagaag tagttactga agttacagca
      661 gattttcttg ttactaaaga cagtgtcaaa tcaaatatgg atgttatagt tgatatattg
      721 actataatta aaaacatttt tctagataaa aaataataag ttaatataaa ataagcctat
      781 aagatataat ttaaacgacc ttaaaatagc atttataagg tcgtttttta ttagcttgat
      841 ttgacactat gtgtttttaa taattggtaa tattcctctt tatcatttga acatatatat
      901 ctatgcttgt tgtttcaatt acatccataa gaacatcttt acattcaagc tcaaaatctt
      961 ccattccttc taaatattca tcatcttcat caaaaaaaat ataagaatta tatagccatt
     1021 cttcaaattt ttcttcaagt atatcccaat acgaactcca aaataatcca caacctgata
     1081 tataagatgc tctactttga tttatataaa cctcctttag aagcttttgt aatctactgc
     1141 tataaagaaa atctgctctt ccactgcttg atattaattc atttaaatct tctattgaca
     1201 ttaattcaac tttaccactt ttatcataag gagtataatt ttcatcaacc catttatgtg
     1261 gtttgttttc tctgcatact gttgctatca actctacaac ttttgttctt atcatttttt
     1321 tatcctaact tatttatttt ttataaaata atcatgattt tataatttat ctatttcttg
     1381 taatgctaat ttaacagttt catatctatc agcaccattt attactacat caccttttac
     1441 aagtttatta gcttctccac ctacaacata aaccttttta cctaaacctt gcttataact
     1501 agacgcctca attattaaag aatctttcaa ttgccaatgt aaaatctgag cagccacttt
     1561 atcagcatca cctgtataaa cgattacatt ttcatacact ttattttccc ctcccccttg
     1621 agaatttgat atagatttgt ttagtatacc ttctgctatc aatttagcaa ctttatcttt
     1681 attattaaca taataatctg tatctgcttt actatctaca aaacatactt ctattaatat
     1741 tgctggtgca atagtatgat taagccaata aagacctcta acatcagact ttgcacccct
     1801 atctttgaat actgtagcta acctagtatt cactctttct gcataggttt tgccattatt
     1861 agtcttatat attgtttctg tccctactgg agttgaagtt gtggcatttg aattaaaatg
     1921 aatctgaaca gccaaatcag ttccctgttt atttgctata gcacattggt ctgctaaatg
     1981 attacttgat tcatcaactc ttcctgtata aacagtagct ccacctattt taagccattt
     2041 aacaattaag tcattcagta ttcttgtttc tttgctttcg tttatatacc caacagctcc
     2101 tgtacctttt ccaattaaag tgtgccctgg tattattact actttcaaac taacatctcc
     2161 tttacaatta aatttaactt ctatttaata tatgcataac ctagaatttt gtttcttttt
     2221 atatcttata cttttattat aacccatttt gtaaaaatat agaatattaa gtaaataatg
     2281 ttaaaattat aattttgtat ataagacatt acttgattgc aataatttta tagcatattc
     2341 atctattcta tatccagttc tataatatat atgtttaatt ccagcttgaa ttatagcctt
     2401 agtacaattt aagcaaggaa agtgagttac ataaatagag caatctttaa ctgaaatacc
     2461 ttcttttgca caataataaa gggcattttg ttcagcgtga atggttctaa tacaatgtcc
     2521 atctctcatt tcatgaccaa cttcactaca atgcttatct ccagaaatac taccattata
     2581 tccagtagac actattctgt tttctgaatt gacaattata gctcctacat aagctctatc
     2641 acaagttcct ctttcagcta ctgtttcgca cagtctcata aaatattctt gccatgagca
     2701 tctattttca agttttttat tcaaatcata tacctccaaa atttatatct aatatataag
     2761 atttatacct taaaaataat attaaaaaca aatttatacg ataaataaaa tttattaata
     2821 ctgtttttag tctaaattat ttcattttaa attaaagtta caaggaatct aaaattatat
     2881 atctttcttc gtaatatgaa tgttacaata taaaaaactg tctcaataaa atagagacag
     2941 ttttacagtc aaagtttata atacaagttc atatttttct ttaaattctt ttacagcttc
     3001 gggccatgcc ttttgttctg taataccttt ttctttagct attttaattg caatctctct
     3061 tatcattgca gcgccttcta atattcccat ttttaatctc ccttcaaata tctttttgta
     3121 tagaatataa atagtgctaa gatttattac atgctgcata tacaatatct atacaacaca
     3181 taacttgtaa attcctttta ttttcatacg tctttaaccg acatataatt atattcaata
     3241 agttttaaaa ttatgaactc aattataatt atttttattt ttttagaatc taaacactaa
     3301 aaattgtagg taaatttgtc ttatgccttt ataaatattt ctacagtttt aattagtaga
     3361 catatctatt tttattataa aaagcgatta aaaatggaga atatcctcat aaaaactata
     3421 tataagcaaa atggttaaag gaaaaagaag aaatttactt gaatgttaaa atgcgttgct
     3481 tgaagcaatg ggcagttttg cctataatat cggtaacaac tgaaagaaag gtggttagcc
     3541 ctaatgttaa gcgaaaacat taaaacaatc aggaagtcaa aaggactttc acaacaagaa
     3601 cttgctataa aactgaatgt agtgcgacaa acaatatcta aatgggaaca aggactgtcg
     3661 gttcccgatt ctgatatgtt gatttccata tcagaggtgc ttgaaacgcc agtaagcact
     3721 ttacttggag aaactattgt tgagtcggaa gttgataact taaaagtgat ttctgaaaaa
     3781 ttagagatta taaacttgca acttgcacaa agaaaaaaca tgagaagaaa aatgtttcat
     3841 tggttattta ttttattatg tgcagtcata ttaatagttt ttgtggtctt agggggacta
     3901 aatagtcctt acttggattg gaattatacc gaccctgaaa ccgctgtttt tggagtagct
     3961 tttcatgctt ttgagtggct atttgtcaga ttagtaccga ttgtccttat aggagcaatt
     4021 attggaatct ttttgacacg taagaaaata tgaagtaact ctgcttttta taatacagca
     4081 agcgttcctt aaaagcgtca ttctacatgg caatcgagga cgactatgta aggaagaacc
     4141 tttttgattt ccaactaagc gaagttattg aggacgatag taaaaccaaa accgcattaa
     4201 gtaaagtaca ggaagcaaga ctgctggact atactctcat gcgtcagatg caggaaatgc
     4261 gaggtctggt agcatgattt acaacgattt gtacaacgac tacgagtaga aatatatgga
     4321 gataagtatt atctacgaaa tcagaaaaga aatgacttga taatttactc atgttataat
     4381 tattgatgtg aaattaataa atcgaaattc cttttttgga tttaacaata ctgagtaagg
     4441 aaaaagtgaa tgacttttgc aaggaggtgt taatatcagg caagtaaagt cttttgtttt
     4501 tccagttata tccttaatat tcttagacca aattagcaaa gttcttatag gattattctt
     4561 aatggacttt gaaattgata taattgggaa atttttaaga ttcaatcctg ttcaaaatac
     4621 aaatctatct tatgggggaa actttattgg tattctatcc aatttatggg tattggtatt
     4681 gtttaacatt ttagttatat tagttattat atctggatat gctttttata aatcaaaaaa
     4741 tgaacaaaca agctattcag taaaagtaat tatgtcttgt ggacttgctg gtacaatatg
     4801 tagcttgata gataaattat tttggggagg aagtttagat tttttgcaga taccaagctt
     4861 ttttattttc gacttaaaag actgttacct tactgttgca gaaataatat ttgttgtcat
     4921 aggaattttg cataatagag aaatatcaat gaaagaatac atatattttt gctatcgtca
     4981 gtttaaaaga taaatttaag tttcaaggta aacttattta atcacagcaa atagttggct
     5041 tacttaagct tttgggctaa actgtgagca ctaattaagt ctctgctatc taacaaacgg
     5101 agcacaaaaa taaaaattaa atattaaata taaagcacag ttaattttga ttaaaaagtt
     5161 gactgtcttt tcttttgtag aaatttaagc ataggtaata aagaaatgac tgcatttaag
     5221 cattatgtat ataattggag gcaataaaaa gccagcaaag cccataaaat agagctgtgc
     5281 tagcaacatg atgagttata aaaagataat caaaaactta cgctttattt taatttaaaa
     5341 aaatgattac tgtaaagaaa tcatttttta ttataatcac tgatacatca aatattctaa
     5401 ataaaatttt tatttatctg gaattgtata ttttttctat aatattaact acagcgtaca
     5461 ttgcccaagc acagatagcc aatacaaata ctcccatcat tactaaatct aatttaaata
     5521 cttgagaacc atatactatc aagtagccta ttccatatct tgaaactaaa aattctccaa
     5581 ctattacacc tacccatgcc ataccaatat taattttagt taaatttatt aaatttcctg
     5641 tgtttgcagg taatatgagt ttaaataata tttgagattt agtagctcca aagcttttta
     5701 acatcttaat tttttcttca tctatattca taaaataatt ataagctgaa agtatagtaa
     5761 caacaacaga aatcgttaca gctgttacga cgattccttc tattccagca cctacccaaa
     5821 ctataattat aggagctaaa gcagtcttag gaagagcatt taaaactacc aaaaatgggt
     5881 ctaatatttt tgataatttt tctgaccacc aaagagcaat tgctaccaaa atacctaata
     5941 cagtacctat aacaagacca agaacagttt catatacaga tattccaata tgtttaaata
     6001 attgaccact aaatgcatat tgaataaata aattgtaaat atcacttggt ttgctaaata
     6061 gaaatgtatt tataacattt aagtcagcta aaagttccca gattacaata aaaccaatta
     6121 aaattaatag ctggtagaat aatacatttc ttttttctct ttttacacct ttaacatagt
     6181 ttatatatcc ttcagatctt ttattaagcc tcatgtgtat ctagctcctt ccataatatg
     6241 tcgaaatact tttgaaaatc aggaacttta cgtgttttga gaggcgtttt tgttccatcc
     6301 atgttaagtt caatatcgta aatatttttt atggtagctg gtcttttaga tagtattgca
     6361 accttatctg atatgcttat tgcttcacca atatcatgtg taaccaaaat agtagactta
     6421 ttctcattct taataatact ataaacatca tcacaaacta ataatctagt ctgataatca
     6481 agagctgaaa aaggttcatc taaaagtaga atatctggat ttacagaaag tgttctaata
     6541 agcgcaactc tttgtctcat acctccagat aattcttttg gatacatact tctaaaatcc
     6601 cataatccat aagtctttaa aaggctttca actctatcta aagatttttt atcttttttg
     6661 ccttgaattt ccaatccaat agttatatta tccattatat ttctccattc aagcaaatta
     6721 tctttttgaa acatatatcc aatatttcca gtaatcttta tatcaccact agtcggtttt
     6781 aaaagattag taagaatatt taatattgta gatttaccac ttccagaagg accaagcaaa
     6841 gttaatattt ctccttcttt caagttaaaa ttgacatcct ttaatacttc aagctcgcct
     6901 tccttagtgt agaagttcat atttatattt ttaacttcta ctatgttgtc attcattatg
     6961 ccacccccta taacttttag ctcgtatata ttatgttatt aacaatatga cctatttgta
     7021 ccatttttca taaatataat ataaatattt tgtttattta cgtattttct tagtaacttg
     7081 tacgtataaa ttatagataa ataaattata aggtagggga taaatttgag taacataata
     7141 acgaatttaa tagagtcaat atttactaat ccaataacta tattgttttt acttgcaata
     7201 actgtgtatt ctgtttttaa aataatagaa aataacagag tatattcatt tgtaagttca
     7261 aaattagacg aaatcaatag agactataag acaagtgagt tttataaaaa ggtaagggat
     7321 gactactata catatagtaa agaaaatcct tatgcagacg taaatataac ttcatttata
     7381 gaggaagttg tatctgattt aaagcataat aatttgccat tgttagaaaa aattagaagt
     7441 ataaaaaatt catcttcaat aagtatatta cttggggttt taggtacgtt tgttggtctt
     7501 tctacaatgc ttttatgtgt tgatactaaa gacattataa attcacttcc ttcaactatt
     7561 agtagtatgc aaactgcatt t
//
LOCUS       DAEMHW010000176         7562 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEMHW010000176 DAEMHW010000000
VERSION     DAEMHW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.176"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            378..1679
                     /locus_tag="KPK67_003077"
     CDS             378..1679
                     /locus_tag="KPK67_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2460006.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1755..2030
                     /locus_tag="KPK67_003078"
     CDS             1755..2030
                     /locus_tag="KPK67_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2460007.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3146..3376
                     /locus_tag="KPK67_003079"
     CDS             3146..3376
                     /locus_tag="KPK67_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2460008.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3369..3950
                     /locus_tag="KPK67_003080"
     CDS             3369..3950
                     /locus_tag="KPK67_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2460009.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSI"
     gene            complement(4529..5206)
                     /locus_tag="KPK67_003081"
     CDS             complement(4529..5206)
                     /locus_tag="KPK67_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG2460010.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5395..6759)
                     /locus_tag="KPK67_003082"
     CDS             complement(5395..6759)
                     /locus_tag="KPK67_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2460011.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTGQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSILPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGMGVRGASIATAISQIASTLVYIGYIL
                     SKKSIFSFRISECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            6887..7408
                     /locus_tag="KPK67_003083"
     CDS             6887..7408
                     /locus_tag="KPK67_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG2460012.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         2956 a          857 c         1297 g         2452 t
ORIGIN      
        1 agtgaacgat atattaataa ttggcagtaa gttatatcaa ataagatatt actatcgagt
       61 aatgaattat attgaacgat atatcaataa ttggtagtaa gttatatcaa ataagatatt
      121 actatcgagt aatgaattat attgaacaat atattaataa tttgtagtaa agtatatcaa
      181 ataagatatt gctattatgt agtaaattat ataaaacaat gtatcagaat aaatagtata
      241 ttaataattt gtagtatatc atgacgaata atacagtcat aacaaatagt gagtcataac
      301 aaataatgta tttataattg ataatgtatt taaaaatcaa aatataaaaa cttataagga
      361 aagagagatt aatttatatg ggtaaatcaa aagatgttga aatagagaag tctattttag
      421 gcacaattct tttagacaat aaattaagct ataagctaga tgaattaaat gaaaacatgt
      481 ttatgaatga tatatgtctt gagatattta aaattatgaa agaacttaaa aaagaaaaca
      541 tagtgattga tgtggccact gtgaaatcaa agatagatag aaaatctcta gcaataaaaa
      601 caagtgatgt gactaatcta attacttggg gtcagaattt tggtcttgat ggtcatatta
      661 aaattttaaa agaaaattta gctagaagaa gcataaatca aaactgccaa aaccttttac
      721 acagcctaaa ccttggagaa aatatcgata cttgtattta caaatttgaa agtaatataa
      781 aggagatttt ggacaaggat acttatgaaa atgatgatgt aaatagtata gcagggaagg
      841 tgcttgattt tttagaaaac aaaaaagata ttggatttaa atttggtata aaattacttg
      901 ataccactat aggaggtctt tttaaaggag agctaaccac aatagcagct aaatcggggg
      961 ttgggaagac tgctttggca cttcaaataa tgttaaattc atttaagcaa ggtaaaaaaa
     1021 ctttatttat aagtagagaa atgaccagtg agcaagtatt tatgagaaat atatgcagag
     1081 taactggtgt aagtacaagg aatatgaaat ctaaagaaat agatgaaaat gattggaaac
     1141 ttattgtaaa tgctataggt gatttaagcg aaaataactt aatttatatc aatgataaga
     1201 tagatacaat tagtgcaatt agaaagagga taaggcaggt aaagccagat ttattaatag
     1261 tggattatgt gcaactcctt acatctcaga aaagcatgga taagagagaa agggaagtag
     1321 ctactttttc gagagaactt aaaaatatga ctttagattt taatatacct gtaatccaat
     1381 taagtcagtt aaatgatgaa atgaaagatt caagacctta tggagataga cctatgaggg
     1441 atagcaaagc catctaccac gatagcaaca atgtggttta tatacatcaa ttaaagggga
     1501 gtgattatga agaagcagtg agagatattg gagaaagtga ggaggcagtt agagcatcag
     1561 aatacagggg aattaagatg gttgatttaa tagttgcgaa gtgtagagat ggacagacta
     1621 ggcataagca tttttgttac tttggggata agttacattt tcaagaacta aattattagg
     1681 tcaataaagt agtaataaca agcttattaa aagtcgggaa atagcaaata gattataaaa
     1741 tatgaggtga aaatatgttg gaattaagaa aaattataga attacttgaa gaacacaaga
     1801 tagaagtaag tattccaaag gagttacata agtatagttt attggaatac cttgaattgt
     1861 gtcaacaaag tgggggaaca tatttttata ttaaaacact ggaaggggct ttaaaagagg
     1921 ctagaaatga gcttatgatt aaattgttta atgggaaaaa ttatatggaa ttagcgagga
     1981 tgtttgaaat gagtgttatt aatgtcaggc atattgttag aaaaaaatag atatttttgg
     2041 agaatacaag tgtgatatac ttctgaaata gaagttatta tagttgaaat aaaattgtgg
     2101 ttaataaatt aaagttgtgt ataagtttaa gattatagag gagtataagt gtgattaaca
     2161 gcatgatttt gtgaataaaa ttgaggatat atatgagtta aatgtgtgat taataaggta
     2221 aggttgtgta taagattatg agtatttaaa aggaggggct ataattaata gtgtagagtt
     2281 gtgtgtttgc ttaagattat ataataattt aatagattaa actatactaa taaggtaaaa
     2341 tcgtgtgttt agttaaagct tattataaaa tcttgatatg tgattaatga tgcaatatgt
     2401 gaataaagtt aagattataa taggtttaaa ttgtgactaa tagagtaaga gtgtaaatag
     2461 agataaactt caatatgagc ttaacctatg attaatagag taagagtgta aatagagata
     2521 aacttcaata tgggcttaac ttgtggttaa tagagtaaga gtgtaaatag agataaactt
     2581 caatatgaac ttaacctatg attaatagag taagaatgta aatagagata aacttcaata
     2641 tgagtttaac ttgtggctaa tagagtaaga gtgtaaatag agataaactt caatatgggt
     2701 ttaacctgtg gttaatagag taaggtgtaa ctaaagataa acttcaatat gagcttaact
     2761 tgtggttaat agagtaagag tgtaactaaa gataaacttc aatatgagtt taacttgtgg
     2821 ctaatagaat aagattgtga ataaagttat agttttagat gggtattaac ctgttactaa
     2881 tagaatatga ttaggtgtaa aattatagag ctgtataaga aaatattata gatatattaa
     2941 atattagata ataaatattt atatagatac acaaaagaaa aggcgatatg aagctaaact
     3001 taatgacttc atatcgtttt ttatttgaaa ctcctttgta tttagagtgt gatttttaat
     3061 attgaggttt tatgtgatta agtcacagat aaaaagtaag tatcttgata taattcttat
     3121 agattaataa tatagggagg ttattatgtc taataaaaaa aagaagtatg caaatatttt
     3181 agcaaatgag tgcgttgctt gtggtagctg tataaaagca tgtccaagaa gtgctatctc
     3241 agttccatgt ggcattagtg caaaaattaa tagagattta tgtgttggat gtgggatttg
     3301 tgaaaagata tgtccagcat ctgtaattga gattataaca atattaaaag aggaagaagg
     3361 taagtgtcat gaataataaa aaacattggt atgactattt atggattttt tcttcaatat
     3421 atttgatatt gggatttgta aatattattt tcgcttggat tgggctaatt tgtttctttg
     3481 ttccactagc tatttctatt gttaagggaa ataaagctta ctgtaataag tattgtggaa
     3541 gagggcaact gtttaatttg cttggaaata aattgaagct gtcaagaaat agagatattc
     3601 ctaaatttat tagaagtaag tggtttagat atgggttttt gacatttttt atggtaatgt
     3661 ttgccaatat gcttttttca acttatttag tgtttgaagg aagtagaaat tttaaagaag
     3721 tagtgacttt gttgtggact ataaaaatgc cttggcaatg ggcgtatcat ggaactttag
     3781 tttcaccttg ggtggtacag tttgcatttg gtttttatag tgttatgctt acatcaactg
     3841 ttcttggttt gattactatg atattgttta aacctcgttc atggtgtgtt tattgcccta
     3901 tgggtactat gactcagatg atatgtaagg caaagagtaa gtctatatag aaaggcttta
     3961 tgtgatattt acagttttgt tcaagtagag atgagtagta ggtttgaata tatatgacaa
     4021 atatatgagt atagaggcat agatttagtt tgtgtaaggt aaactgattt atgcttcttt
     4081 tttatgtctt agattattgg ctaagtggaa acaatgaatt tagatattaa gaattattca
     4141 gttatttgaa gaaaatataa taataattaa cattatatga atggatattc attacatata
     4201 agacagttaa tagcaagatt tatttaatat gaaataggtc aagtatttaa actaaataag
     4261 gtcatgacaa ttaatataag tatagataca aaaagattag aagttataac ttataaaagg
     4321 tattctatgt aaataatatg ggaaaaatag tcaaattaac ttattagaaa caaaaaatat
     4381 acataaaaaa taaatgtaaa tatcaaataa gatactatat caaaatagat ataagtatct
     4441 tttttattat tgaaattatg aaaatatatt tgattttaca caaaaaagag tgcctcatga
     4501 actaaaaagt tcattttgag acacactttt acagtgtcaa agaacgaagt ttatccttat
     4561 ccaatacttt tataccacca cgagataaag acacaatacc ttctctagca aaatatttca
     4621 acattctcga tacaacttct ctggcactac ccatatactt agcaatttgt tcatgagtca
     4681 tatttatagt gctactgcca tttttagcta tctcatcaat taaaaaagat gccaaacgct
     4741 tatcaaaact cataaaaaga atttgttgca tagcccacat aacatcagaa aaacgttcgg
     4801 tagctaattt atatgaaaat aactctgcat gaatattttc tgtagataat ttcgcaaaaa
     4861 cagaggaact aatctgtata atatcgcagt ctgtttcagc atctacatag acatcaaaag
     4921 ttattgtttt tagaatacag gaagctgata aaatacatac atctccatca tcaagtcgga
     4981 acagtgtaac ctctcgacct tcatctgaaa gtatgtatgt acgtactgtt ccagacttta
     5041 taatcaaaat accaatacag tcactatcac cacaatgtac tggattccct tttttgtaat
     5101 gcgaggtatt tgctgaagaa attaataatt ctttttgtaa gtcagtaagt ttatcccaaa
     5161 aaggcaagct gtgtgtaagt atatcaattt cattttgtga aatcatacaa tatctcctct
     5221 cttaaattgt tataatccca tgatacaagt gaatagataa attgacaagt agtttgttaa
     5281 gaggattatt tagtcagata gtaatagtat gatttatgta gaaaaaatat gataatatat
     5341 attcttgcta ttaatgagaa agtaatatgt tttgatgata gtaggtttgt ataactaatg
     5401 cataccgttt atttttaaaa gattaagttc cttatgtaac cttatagaca taaaaccagc
     5461 aattatagca gccatcaaat cagctattgg ttgtgcatat aagataccat ttaacccata
     5521 gaatacagga ataatcaaaa ttatagggat aaaacagaca ccttgtctac acatacctaa
     5581 aaatccacct tcctttgctt ttcctagtgc gaggaataat gtagaataca ctgtatagaa
     5641 tccaaatagc ataaatgaaa acccatttgc cctaagagct ttactaccat tttctattaa
     5701 taaaatgtca ccacttgtaa attgtgaaat tatagatttt gggaagagta tcaatataag
     5761 accaagcact acacaaaata ctgtagtcca taatatggaa gtttttattg ctatatgtaa
     5821 tctatcgtag ttctttgaac caaaattata accagcaatt ggttggaaac cttttataaa
     5881 accaaataca acaagactac ctaaagatgt tattcgtgtt acagcaccca ttccagctat
     5941 cacagaatca ccatactttg tagcctccat attagttaat gtaatggcaa gactagtcaa
     6001 caattgaaat attaaggtag gtatgccaat ttttaagatt tctgaaaaaa tttcttttga
     6061 aaaacaacat tcactaattc taaaactaaa tatacttttc ttgcttaaaa tatatcctat
     6121 ataaacaagt gtcgaagcaa tctgtgagat agctgttgca atagaagcac cccttacacc
     6181 cataccaaaa gtataaatga atatcgggtc taaaatcata ttaagcaaag cacctaataa
     6241 catagcacac atagttgttt tagcagctcc ttcacttgtg acaatattag tcatagtgac
     6301 gttgaatata ttaaaaatag atgatactac ataaatgcta gcataagata ttgcataagg
     6361 caggatactt tttgtagctc ctaacaattt taaaattgga ttgagaaaaa tcataatcac
     6421 aataataact aatgtaccca ctataatact actgtaaata gatgtactga ccactttccc
     6481 tgctttttca ttatcaccat gacctaaaag tctggcaata tatgaagcag ctccatttcc
     6541 aaacaataat cctaatccca caacaagctg acctaatgga aatgcaacgg atatagctcc
     6601 catttgacct gttccaagtc cacccacaaa gtatgcatca actaaattgt ataatgcagt
     6661 tatcatcata cctatcatgg taggtaatcc catagataac aatgctttcg agattggggc
     6721 agaccctaat agttcaattc ttttgttact ttctttcata gttaacactc ctattctatt
     6781 gacattaagt ctaaattata atactataaa ttatagtaag tatggaaaat agtactataa
     6841 tttatagtat ttgtcaatag taagaaaact aaaggagatg atttttatgg ctacaataga
     6901 tttaattgta ttgggaatag taaagcaaca acccaagagt gcttatgata ttcaaaaggc
     6961 agtggaatac aggaatatat cacgttgggt taaaataagt acaccatcaa tttataaaaa
     7021 agtaattcaa ttagaacaaa agggatatat tgaaagtaaa actgtgaaga atggaaaaat
     7081 gccagaaaaa gcagtttatt cattaactga attaggtgag acacattttt tagatttaat
     7141 gtttgataca tctagcaaac caataaatat tcttttagat tttaatgctg ttatagtaaa
     7201 tttaaatagc cttacaccag aaaaacagaa attatgtctc aataatattg aagagaatgt
     7261 tcaagagtta aagaaaacta ttgaggaaaa tataagtata aagacagaag taccagagac
     7321 agcaaaagca gtgttggagc aacagttggt gctagcacaa gcattggaaa cctggataaa
     7381 ttctttaaaa gataatatgg gaaattagaa ttagagaagc taacttaata atcattaaat
     7441 aaaaaccagc atatttaata atgagatatt agaaaaaata aaacaaatga tataaactga
     7501 aaagtatatt taaaataaag ctcatttaat tgagggagaa tgttaaactt tttaagagca
     7561 ag
//
LOCUS       DAEMHW010000177         7532 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEMHW010000177 DAEMHW010000000
VERSION     DAEMHW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.177"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            269..1081
                     /locus_tag="KPK67_003084"
     CDS             269..1081
                     /locus_tag="KPK67_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2460013.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            2129..2890
                     /locus_tag="KPK67_003085"
     CDS             2129..2890
                     /locus_tag="KPK67_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG2460014.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            2955..3737
                     /locus_tag="KPK67_003086"
     CDS             2955..3737
                     /locus_tag="KPK67_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG2460015.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            4004..4423
                     /locus_tag="KPK67_003087"
     CDS             4004..4423
                     /locus_tag="KPK67_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBG2460016.1"
                     /translation="MDKISGKLTVLFEEPFWVGVFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDKIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLTKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(4526..4792)
                     /locus_tag="KPK67_003088"
     CDS             complement(4526..4792)
                     /locus_tag="KPK67_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBG2460017.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSMIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            5004..5705
                     /locus_tag="KPK67_003089"
     CDS             5004..5705
                     /locus_tag="KPK67_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460018.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYRGAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            5787..6395
                     /locus_tag="KPK67_003090"
     CDS             5787..6395
                     /locus_tag="KPK67_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG2460019.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            6719..7183
                     /locus_tag="KPK67_003091"
     CDS             6719..7183
                     /locus_tag="KPK67_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HBG2460020.1"
                     /translation="MKKLVIILISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNIWH
                     GSVDNDTFAISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
BASE COUNT         3069 a          673 c         1273 g         2517 t
ORIGIN      
        1 aaaaatatga aaggatgatg ttgttcggtg atatttgata tcataaaatc gttgatataa
       61 gtattgctgg aaaggagtga ataaaatcaa atttaataag caaacaaagt aaagctatag
      121 ttttattgtt gtgcttaaaa atatttggtt ctattttact cttatttaaa ctcatctttt
      181 tgaagttaaa ccacagagta aactctgtgg tttttttatt ttaaaaataa attttaagga
      241 gtggtatgtt ttgttaaaca aattatgtat gtatttagaa agaccagagc tttataaaca
      301 aagtgaaatt aatttttggg atgatgagta tatttcaaag caattattaa aagcacactt
      361 agacactaat tttgaaggag caagtagaaa ttttaacttt atcgaggatt ctgtaaattg
      421 gatagttaca gttgcaaatc cagctaatta tcctaaatta cttgatttag gttgtggtcc
      481 aggtttatat gcagagaaat ttgcacaaaa aggttataag gtaactggaa ttgatttctc
      541 aaaacgttct attaactatg cacaaaatag aaataaggag actaatttaa acataaatta
      601 cttgtttcaa agttatttaa atatgaacta taatgaggaa tttgatttgg ctactttaat
      661 ttattgtgat tatggagctt tgtcaactga aaatagaagg ttattaatgg aaaaaattta
      721 tgatagttta aaacctggag gaaaattaat attagatgta tttacaataa ataaatacaa
      781 taattttgag gaaattaaat attgggaaat aaatgaagat ggaggatttt ggagtaatga
      841 aaaatatatg tgtttacaag ataattgtaa atacaatgat tataatacat tagagcaaac
      901 tcttgtaata accgaaaaag atgaaaatgt attttatgtt tggaatcact atttttcaaa
      961 agaaagtttc ttattagaag ttgaaaatat tggatttaag tctgttgagt tttttagtaa
     1021 tgttaagggt gaagcatatt ctgacgatag tatgactatg ggattagttt tacaaaaata
     1081 ataatttttt taaaatgaat aaataaaaac ttgaaacctt taacaataaa ataacataat
     1141 aaaagcttac atattgaaaa atggaaactt ttatgttatt tttagtacat tacaaatttg
     1201 cttatataaa aatcaatact tgaattatta tttttatgtg aaatttatct atagtatatt
     1261 tttataaaca agtaatgtta ttaaaattaa gtatgtagtt aaaagtgtat agcaaaaagg
     1321 ctgttatatt aatgttagag gttcagggaa aatatataaa gcaaagtatt tacagataat
     1381 gtagattgca cagctataag ttagataata gaattatgta atcaagaatt tacaacaaag
     1441 tataagataa ggataatgct aaattgccat acaggtgcta gatgtgtaaa tggatttaat
     1501 taatactatt gataaagtgc aacaagcaca taaaccaatt gatgagatta ttaaaactat
     1561 aagagatata gttgacataa ttaaaatatt ggaacctgtt tataatttta aatcaaatca
     1621 ataatgatta agattaaaat aatattaagg catcaatgtt ataagagtac aatataagga
     1681 gtactatata gtactcctta cttatacata ttaggcaata ggaaattttt tcatagttaa
     1741 attttcaaat attttttagg gaactttata attgagaatt aagggtatag tatgtgatga
     1801 acttactaaa tttataatta ggttatataa tggaataatc atcaatagtt ttactttagg
     1861 gaaaatatat gcttaaggaa gtatagctta ctaatattat taaaatgtat atttaaaatg
     1921 ataaaaaaag aattagacaa gtatttagta atttgattat gcaaaattct atcaatcagg
     1981 aaccctatga ctttagtcat gggaggttca gtaggtaact tgttgaatta agtttatatt
     2041 atttaaataa actacttaat ttagataata taaaaagtca gaattttaag aatagagcta
     2101 tataatctta gctattagga gaagtactat gataggaata tattttagtg gaacaggaaa
     2161 ttcaagatac tgtgtagaaa aatttttaca agaatatgat ataacagcaa attcttattc
     2221 tattgaagat aatgaacttt tacagcacat caataatcat gagaatatta tttttagtta
     2281 tcctgtgcaa tatagtaatg tgccaaaaat attgaaagat tttataataa atcattcaac
     2341 tttgtggaat ggtaaacgtg tttttattat tgcaactatg gctctattta gtggagatgg
     2401 ctctggtgtg ttatctagaa tattaaaaaa atacggtgct ataactgttg gtggattaca
     2461 tgtgaagatg ccagatagta taggagatga aaaggtatta aaacattctt tagagtataa
     2521 tagaaagctt gtaataaaag cagaaaagaa agtaactaaa gctgctaaaa aactgaaaca
     2581 tgggtcacca ccaaaagaag ggttgggttt tttgagtcat attgtagggt tatttggaca
     2641 acgattgtat tttatcaata aaacaaatat gtatacagat aaactaaaaa ttaatactga
     2701 ggaatgtatt gtatgtggta aatgtgttaa tttatgtcca atgaagaatc tagtaattaa
     2761 aaattgtatg attgttgcaa atgggcaatg tacaatgtgt tatcgttgtg taaataattg
     2821 tgctaaaaaa gcaattacat tgttaggtaa aaaagttgtt gaacagagta atattaaaaa
     2881 atatatgtaa gaaatttaaa gttttattga atgtaaattg atgtatattt tatgttagaa
     2941 agagaggtta aataatggaa tactacatag ttgattcatt tgcaactaaa ctatttaaag
     3001 gaaatcctgc tggagtttgt gttcttgaca gaagaatacc tttagagtta atgcagaaaa
     3061 tagcagaaga aaacaattta ccagaaacag catttgtagt taaaggtaaa ggaaattatg
     3121 aattacgatg gtttactcct aaagcagaaa ttgatttatg tggtcatgca actttagcag
     3181 cagcttatgt aatatcaaat tttattgatg ttaatgtaaa aaggatagat ttctttactc
     3241 aaagtggaaa gcttgaagtt acacgaaatg gaaatctata tgaaatgata tttccagaaa
     3301 taatgcctat tgaaatagag ttatcaccac aacaagctaa tttgataggc tgtgtaccaa
     3361 gtgctgttta ttcttcaaga gatttaatct tattgctaaa cagtgaacaa gaagttataa
     3421 attataaacc taattacgca caattgcgta aattgacaga ctggttaggt ataataatca
     3481 cagcacaagg aagtaataca gattttgtat caagatattt ttgtccagaa ttagattcag
     3541 aagaccctgt tactggttca tcacattgta atcttattcc atactggtct gaaaagttgg
     3601 gaaaacacaa aatggtagct gctcaacttt ctaatcgtgg tggtataatt cagtgtgaag
     3661 tgttgaagga taatactgtg aaaatttctg gtgaagcagt cttgtttatg cagggaacaa
     3721 taaagattga tatttaaata agtataataa ctaatttatt atgaaaaata attaattttc
     3781 ttagtgttaa caataataaa gtagagttaa aaaacttgat ttcacttaaa tcaatatgat
     3841 agatgttgat actaagattg acaagcatga ttaaaaaaga taatatttaa tctataaaga
     3901 taaaaacaca gtctggttgg tagtccagac gcagcaatag ctgtcagtaa cctgcctcct
     3961 tggttgtccg ttctttagaa agtaatttaa ggaggaacta gcaatggaca aaattagtgg
     4021 aaaattaaca gtcttatttg aagaaccatt ttgggtaggt gtatttgaaa gacaagatgg
     4081 taaaaaatat gaagcatgta gggtagtttt tggagcagaa cctaaagaag tagaagtgta
     4141 tgaatttata cttgaaagat ttttctcatt agattttggt agcattaaat tagaaaaaaa
     4201 tgtgaccaaa gacaaaattg gatacaaaag aatgcagaga aaagtaaaaa aggaacaaga
     4261 aaaagaaact atagggacta aggctcaaaa tgctcttaag cttcaatatg aagaaagaaa
     4321 acaggataga aaaaacctaa ctaaaaatag aaaagaagaa gaaaaagaaa gactatttaa
     4381 tttgaaacaa gaaaagagaa aagctaaaca taagggtcat taattttaat taaatatctt
     4441 tttaataatt gctagaaaag ggtgtataaa gtttttaatt ttatacacct taatttattg
     4501 aaaagaataa ggttttatat ttcaattact ttaaaatata aaaatatact aataaaaatt
     4561 gtaaaaatag tataaatggg ataccaaatg taaatttaat gtgtttagtt ttatgtctaa
     4621 aagaatacat tccaatcata gaacctatac tacctccaat tactgctata gacataagag
     4681 tagcttcttt tattctccat tcgtttctaa ttgctctttt cttatctatg tacattgaaa
     4741 agaaagcaat agaattaatc actataaaat atattagaat aaaatttttc acagtcttgc
     4801 ctccataaaa atatattata cattataaaa caagatattt gttttggaaa gttaaatttg
     4861 taattagtac atataaaata tttgatatta attggaaaat ttaaaaaaat taaacaatta
     4921 gcaaataggg ggtatattta taagacacaa agtgataaaa tctatttaga aaataatgta
     4981 aaataacagg aggataaagc aaaatgaaat ttatagtaga aaaagaagtt tttgataaat
     5041 tagaaaatgt atgctttggt gtagtagttg caaaaggaat agataatact aaagaaatag
     5101 aaagaataag caacctttta gatataagta tagatagagt agaagattac ttcaaagata
     5161 aaaaagtaaa ggaatcagaa gaaattatac catatagagg ggcatttaga agtctaggca
     5221 tgaacccaaa taaatttatg agttcgatag aagcaatgac aacaagggtt gctaagaata
     5281 agaaacttcc tcatataaat cctattgtag atttaggaaa ctctatttct ttaaaatatt
     5341 tacttccaat gggtgctcat gatatggatt ttagaaatga tgatgtatat gttagatttt
     5401 ctaaaaaagg ggataaattt gtaccatttg gagaaactga tgttgaatta atggaagaag
     5461 gagaattaat ttattcagtt ggagacatgg taaaaactag aagatggata tggagacaag
     5521 gagaagaagg aaaaattact aatagctcaa aaaatatatt ttttcctata gatggtttta
     5581 ctgatgctaa tttagataag gtaatgagtg caagagaaga attagcaaaa ttgttaaagg
     5641 aaatatttaa ttgtgaaatt aaagtaggtt ttgtagataa agataatcct gaaatggaaa
     5701 tataataagt tgttgaatac ttgttaagat attatattta cttaatgatg ttaaaaaggt
     5761 attaaaaaag aaaggtcgta gtaaatttga aaaaaactat aataaagaat gtaaatccaa
     5821 tgattgaaaa taagtttata ggactttttg aaatagagta taaaaacaaa ttagatgaag
     5881 ataaggtttg gatggtagca tcaagaaaaa gtagtgagca attaaaaaat atttatttga
     5941 agaatgaaga agatagtgta gatgcagttg taatagttgg gcttcataaa agtagtaaaa
     6001 aattaatttt aataagacaa tttagagtac ctattaatgg atatatatac gagttaccag
     6061 caggtcttgt tgatgaagga gagtctatag atatttccgt tgaaagagaa cttagagaag
     6121 aaacaggact tactctttta gaaattaaaa aaaataaaag tattgataaa gtttatttat
     6181 ctcctggaat gagtgatgaa tctgttgcct ttgtttattg tatatgtgat ggtgatataa
     6241 cagatgaatt tttagaacca gatgaagaga ttgaagcgct tttaatttcg caagaagatg
     6301 caaaagagat tttacaaagt aatcataaga tagacacaaa agcatttttg attttacaaa
     6361 tgtttgtgag tttaggagct aaattatttg aatagatata tagaaaagag gttatataga
     6421 agagtaaaat aataaagttt attttacttg tataaattat aatctctttt tttatacaat
     6481 aaagtataat tttgtgtatt tttaaataag tgacttgact cttaaatttt cataatatgt
     6541 tataaaccaa tttaagatac tacaatagtt aaaattccaa aattatttaa catatttgca
     6601 attaaaatga gaaaaggtaa aggctgaatg attgaaaaat ttgatgcaaa atagtagatt
     6661 agtttaatac tatagtttgt agggttaaaa attagaaatt gtaagggaga taaattctat
     6721 gaaaaaatta gttattattt taattagtat cattagtgtg gttagcttga cagcttgtaa
     6781 aaaaagcaat gaaagtaaat ataataattt aaacaacaaa gaatcaggac aaacaaccat
     6841 tgaaatggac ttagataaaa attatgatac ttcagaccca tttgttaatg cacgtttgtt
     6901 ttgtgtgtct aatgatatag atgttttaga cacagagatt tcttttaaaa tggatggaga
     6961 tagtggtatt gtggaaataa aggacaataa aacagatgaa actttatgga gtaatatatg
     7021 gcatggaagc gttgataatg atacattcgc aatttctctt tctaatatac aaaaagagaa
     7081 agagtatgct atatgcttta ctggaattaa aataaatcat gcagttgtaa aggttacttt
     7141 tgaaagcaat ttagttaagg agaaagaaaa accctcaaaa taaattccag attgtctata
     7201 acttgatatt cagtacataa aagcagttga tatttatgat tgactgcttt ttcctataaa
     7261 aaaggatagg ttgtgcattt ttacttgaaa tagtttatgc acatcaaaag ttggtgagaa
     7321 tgatattgga aatggtaagc atgaaatctt gataacacaa ggaaataaat ttaagttttc
     7381 aattaagtat atttccataa tttagttaac aataagctaa ttattattta tattttgata
     7441 tataaataat aattgtagca aattttaaaa aatcatatat tttttgaact aaataaaaat
     7501 ttgtttttat tattttaaat taggggagat tc
//
LOCUS       DAEMHW010000178         7416 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEMHW010000178 DAEMHW010000000
VERSION     DAEMHW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.178"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            64..1041
                     /locus_tag="KPK67_003092"
     CDS             64..1041
                     /locus_tag="KPK67_003092"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBG2460021.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTDFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1034..2002
                     /locus_tag="KPK67_003093"
     CDS             1034..2002
                     /locus_tag="KPK67_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBG2460022.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2186..2776
                     /locus_tag="KPK67_003094"
     CDS             2186..2776
                     /locus_tag="KPK67_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2460023.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2795..3565
                     /locus_tag="KPK67_003095"
     CDS             2795..3565
                     /locus_tag="KPK67_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBG2460024.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3702..4670
                     /gene="dusB"
                     /locus_tag="KPK67_003096"
     CDS             3702..4670
                     /gene="dusB"
                     /locus_tag="KPK67_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBG2460025.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEKEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4779..5258
                     /gene="greA"
                     /locus_tag="KPK67_003097"
     CDS             4779..5258
                     /gene="greA"
                     /locus_tag="KPK67_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBG2460026.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5273..6802
                     /gene="lysS"
                     /locus_tag="KPK67_003098"
     CDS             5273..6802
                     /gene="lysS"
                     /locus_tag="KPK67_003098"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBG2460027.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNSNKEEE
                     N"
     gene            7111..7305
                     /locus_tag="KPK67_003099"
     CDS             7111..7305
                     /locus_tag="KPK67_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460028.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDSFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
BASE COUNT         2948 a          750 c         1506 g         2212 t
ORIGIN      
        1 taatgtataa ttatttatta gaagaaaata agtcatgaaa ttatgtcata ttagaggtga
       61 aatatgcgag ataaaataat agagattata ttaaataata aatctgaatt tatttcagga
      121 gaagaattat caaaacaatt aggggtgtca cgtgcagcta tatggaaaca tatgaaagcc
      181 ttaaaagaag agggatataa tatagaatct gtaaataaaa aagggtatcg attagcggaa
      241 aatccaactg atttattaag tccacagaat atttactata aattagatac agattttatt
      301 ggaaaaaata ttgaacatct tgacactata gattcaacaa atgaacatgc taaaaggata
      361 gctataaata gtgtagatgg gactgtgatt ttaagtgaac atcaagatat ggggaaagga
      421 cgattgggaa gaagatggga ttcaaaacct catgaaggaa tctggatgag tattatatta
      481 aaaccagata tggaaccatt caaagctcca tttataactt taatagcagg agcaagtgta
      541 acgaaagctt taaacaatct aggagcaaat acattaataa aatggccaaa tgatgttatt
      601 ttaaatggaa aaaaaatatg tggtatatta acagagttat ctgctgaaat tgaaagaaca
      661 aattatgttg ttcttggaat tggaataaat gttaagataa tggattttga acaagaaata
      721 gctgacaaag ccacatcatt gtataaagaa ggatatatgt tatccagagt agatattgtt
      781 aggaatgtat tgactgaatt tgaaaaatta tatttagatt atactgtaaa taattccaaa
      841 gaaaaaacct taaaaatatg tagagattat tctgcaatta tagataaaaa tatatatgta
      901 ttaaaaggag ataatagaga actagtgaaa tgtttagaca taaatgaaga gggaaattta
      961 ttagttcaaa gaagtgatgg aagtataaaa gaaattattt caggtgaagt ttctataaga
     1021 ggagaaaaag gatatgtata ataatatttt ggatatagaa ggcttaaagg ttgggcaggt
     1081 tgaggataga aatggtctta ctgggtgcac agtagtaata tgtgaagaag gagctacttg
     1141 cggtgttgat gtaaggggct ctgctccagg gactagagaa accgatttat tagaccctgt
     1201 aaatatggtt caaaaagttc atgcagtagt tttggctgga ggttctgcat atggtcttga
     1261 atccacttgt ggtgtaagta gatacttaga agaaaaagga attggctttg atgtaggtgt
     1321 aggtaaagtg cctatagttg taggagcagt tttatttgat ttaggagtag ggagctttaa
     1381 atgtagacca gatttaaaga tgggttatag agcttgtgaa gttgcaaata ctgatatttt
     1441 aaatcaagga aattatggag caggttgtgg agccactgtt ggaaagataa gaggtcagga
     1501 atatgctatg aaaggcggaa taggtagcta ttctataaaa ttagataatg gtctaattgt
     1561 atctgcatta attgctgtaa atgctcttgg agatgtttat gaaaatggta aggttatagc
     1621 tggtgcttta aatgataaaa agacagaaac aattgataca tataaattaa tgaaacaagg
     1681 tgaaagtaaa ggtggattta gtgtagataa tactacaatt gggataattg caacaaatgc
     1741 taagttaacc aaagctggat gtaaaaagat atctcaaatg ggacatgatg gatatgctaa
     1801 gtctattttt cctatccata cacctcatga tggagataca atattcacaa tatcaacagg
     1861 agaaatagaa gcagatataa cattagtagg ttcattggct gtagaggttg tggaaaaaag
     1921 tataataaat gcagtaaaaa aagcggatag tgtagaaaat ataatggcat ataaagatat
     1981 agtaaaaaaa cgagaaatat gatgaaatta gcatattttt cttgtcaaat tgttgacaat
     2041 ggcaactcat cttgataaaa ttattcttgt tagagcgggc atcctacaat ggtagcttga
     2101 ctcatagtta taagaaaaaa acgaaaaaaa ttaaaaaatt aagtagtccg gcatcttaat
     2161 gagctggaac ggaggtgtat tttttatgat gaacacaagt gcatcagtaa gaaaagtaaa
     2221 tgtaagaaag atgactataa taggagtact atcagctatt tctattatgc tatctatgac
     2281 tccattggga tttattccag ttggacctac aaaagcaact ataatgcaca taccagttat
     2341 tattggagct attatggaag gaccaatagt tggggcagca ataggattta tatttgggat
     2401 atcaagtctc ttaaatgcta taacaaaccc aacagtaaca tcttttgttt ttataaatcc
     2461 attagtttca atattaccaa gagttatgat aggtgtatta gcttattatg tatatcagtt
     2521 aataattaag gctacaaata aggtatacat atcaggttta attacagggg caataggttc
     2581 tctgttaaat acagctggag ttttaggaat gatatatgtc ttatatgctg acaagtatct
     2641 tcatgcaatg ggtaaaagtg gttcagcagg aaaagtaata atggctcttg ccgcagcaaa
     2701 tggggtgcca gaagcaatag taggggcttt agtagtagct gctgttgcca ctgtacttaa
     2761 gaaaagcaaa aaatagaaag aaggttaatg acatatgctt ctagtatttg atgttggaaa
     2821 tactaatatg gttttaggta tatataaagg tgacaaatta gttaattact ggagaattaa
     2881 aacagataga gaaaaaacgt ctgatgaata tggaatcctg ataagtaacc tatttgatta
     2941 tgataatgtg aatataagtg atattgatga tgttataata tcatctgtag ttccgaatgt
     3001 tatgcattct cttgaaaact tttgtataaa gtactgtaaa aaacagccat taatagtagg
     3061 tccgggcata aaaacaggtc taaatataaa atatgataat ccaaaacaag ttggggcaga
     3121 tagaatagtt aatgctgtag cagggataga aaagtatgga gcaccaagta tacttgttga
     3181 ttttggaaca gcaactacat tttgtgctat ctctgaaaaa ggtgaatatt tgggtggaac
     3241 aatagcacca ggaataaaaa tatctagtga ggcgttattt caaagtgcgt ctaaattacc
     3301 tagagtagaa ttagctaagc caggtatgac tatttgtaag agtactgtat cagccatgca
     3361 atctggaata atttatggat atgttggttt agttgacaaa ataataagta tgatgaagaa
     3421 agaattgaat tgtgatgatg ttaaggttat agctacaggt ggattagcta aactgattgc
     3481 ttcagagacg aaaagtatag attatgtaga tggtttttta acactagaag gattgagaat
     3541 aatatatgaa aaaaaccaag aataattaat atacagggaa ttgggttata tatccaattc
     3601 cctttttatg atagatttct agctatatgt ggtatattat aatgaggaaa taagaattta
     3661 aagttaaatc acaaaataaa ggcaaaaata ggagaaaaac aatgaaaata ggaaatttag
     3721 aattgaaaaa taaagttttt ctatcaccta tggcaggtgt aacagacctt ccatttagat
     3781 taatttgtaa agaacaggga tgtgggcttt tatatacaga aatgataaat ggtaaggcac
     3841 tttgctatga tgatgaaaat actaaaaaaa tgcttaagat agaaaaggaa gaacatcctg
     3901 tagcagttca gatattcggt tcagaaccag agtttatggg aagagcagca gagataatga
     3961 atgattattc aaatgagatt ctcgatataa atatggggtg tccagctcca aaagtagtta
     4021 aaaatggaga tggttcagca cttatgaaaa atcctaagct tgcagaagag gtattgaggg
     4081 cagtagtgaa gaactcaaaa aagccagtga ctttaaagat tcgaaaaggc tgggatgata
     4141 atagtgtaaa tgctgttgaa atagcaaaga tagcagagga ttgtggaata agtgctttag
     4201 caatacatgg aagaactaga gaacagtttt atacaggcaa agcagactgg gatataattg
     4261 cagagattaa gaaaaatttg agtatacctg ttataggaaa tggtgatgta tttacaatag
     4321 aagattcaat aaatatgtta gacaaaactg gttgtgatgc tataatgata ggtaggggtg
     4381 ctcaaggtaa cccttggata tttaaaagaa taaaccatta tatgaataca ggcgagattt
     4441 tgccagagcc aactcttaac gaaaaaataa gtactgccat taagcattta aaacttgcag
     4501 tagaagaaca tggagagtat gtagctgtaa gggaaatgag aaaacacatc gcttggtatt
     4561 taaaaggact tagaaactct gcaaaattaa gggatgaaat taacaaaata gaagactatc
     4621 aagaagttgt gtcgaaattg gagtactata tggaagactc cttgacataa aaataatagt
     4681 gacttataat atagcgcata aattaaaaaa agattataaa atttttaaaa tataattagg
     4741 aaaattccta agaaatttgt gaaggagttg tatttataat ggaagaaaat aaagagtttt
     4801 tattgacaca agagggatat gataaattag aagaagaatt agaaaactta aaggttgtaa
     4861 agagaaaaga agttgcagag agaataaaag ttgctatatc ttttggtgat ttatctgaaa
     4921 atgctgagta tgatgaagct aaaaaggaac aagcacaagt tgaagaaaga attttaaaat
     4981 tagaaaatat ggttagaaaa gctgttatca tagatgaaag caaaatagac cttaatgttg
     5041 ttacaatagg atcaatagtt aaagttaagg atttagaatt tgatgaagag gttgaatata
     5101 ctatagttgg ttcgacagaa gcagacccat atgatggtaa aatatcaaac gaatctcctg
     5161 tagggaaagc acttttaggt agagctgcga aagaagttgt ggaagtgcaa gttcctgatg
     5221 gagttgctaa gtttgaaata ttagaaataa gaagataatt tggaggaaaa aaatgaaaaa
     5281 taatcaacaa agcaatgaag aagcgcaaat tcaagaagac cttagtgaag tacttcaagt
     5341 aagaagagat aaactaaaaa aattacaaga gtcaggtaga gatcctttta aagaaagtag
     5401 atatgataga actcattatt ctatggatat aaaagataac tttgattctc ttgagggtaa
     5461 aactacaaaa atagcaggac gtataatgag caagagaata caagggaaag ctggctttat
     5521 agatattcaa gaccaagagg gaagaatcca atcttatgta agactagatg ctataggaga
     5581 ggaagaatat agtgtatttt caacatatga tattggagat atagttggaa ttgaaggaga
     5641 aatctttaaa actaaaaaag gtgaaatatc tgtaaaggct aaatctgtag tattactatg
     5701 taaatcttta caagttttac cagaaaaata tcatggatta aaagaccaag aactaagata
     5761 tagacaaaga tatgttgact taatagtaaa tccagaagta aaaaatgctt ttttaattag
     5821 aacaaaagca ttaaaagcat taagagcata cttagatgat agaggatttt tagaagttga
     5881 gactccaata ttaaatacta tagcaggtgg ggcaaatgct agaccattta taactaatca
     5941 taatacttta catataccaa tgtatttaag aatagcgaat gaattgtatt taaaaagact
     6001 tattgttggt ggatttgata aagtttatga aatgggaaga atgtttagaa atgaaggtat
     6061 ggatttaaaa cataatcctg aatatactgc aatagaatta tatcaagcat atgcagacta
     6121 tactgatatg atggaaataa cagaaaatgt tattgcacat atggctgaag ttgctacagg
     6181 aagtatgata gttaactatc aaggaacaga aataaacttt acaccacctt ggaaaagaat
     6241 gagtatggaa gattgtgtta aagaatattc tggtgtagat ttttctacta taaatacaga
     6301 tgaagaggca ctagaagttg ctagagaaaa aggtatagag ataaaacctg gaatgagaag
     6361 aggggaagta ataaatgctt tctttgaaga atttggagaa gataaattaa tccaaccaac
     6421 atttataact catcatccag ttgaagtatc tccattatct aagagaaatg ttgaagaccc
     6481 aagaagaact gatagatttg aggcatttgc taataagtgg gagttagcta atgcattttc
     6541 tgaacttaat gaccctattg accaaaaagg aagatttata gaccaattaa gaaaaagaga
     6601 attaggtgat gatgaagctt ttgaaatgga tgaagatttc ttaaaagcat tagaggtagg
     6661 tctacctcct acaggaggac taggaatagg tatagataga gtaattatgt tattaactaa
     6721 ctctccatct ataagagatg tattattatt cccgactatg aaacctattg ataacaattc
     6781 aaataaagaa gaagaaaact aagaaatatt agcattctac taagttttaa ataaagttta
     6841 gctattaaat tatcaataaa tagctaaatt ttatttaaaa tgtcagtgta gaatgctttt
     6901 tatatgcttc aaagattctt ttagatattt aatttataaa ttaccaacaa acgttagaaa
     6961 ttgatattag tttttgttga ttgagacttt atatttagaa aatatgattg aaagtaattt
     7021 attatgaaaa tatttagaat aatttatgag aaggtaaaaa gaataattta tgagaatact
     7081 atatataaaa aattattggg ggaatgtctg atgtttcaag taattaattt tttatgttta
     7141 ttttgtgttg gatttttggt tcttgatgtt atgatatatt tttgtaagga tgcaatggat
     7201 agttttgagg gtacaactaa tgttaataaa gttaagagta taaatgtttg ggcagataga
     7261 gtgagagcta attcatattc ttatgaaaat aaaattgcaa aataggtaaa attatatatg
     7321 catgtattgt ttgtaatatc aagggttata tctattagtt ttaattatat ttattaaaaa
     7381 aaaccaaaag ttataacctt ttttaaaata aaaaac
//
LOCUS       DAEMHW010000179         7400 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEMHW010000179 DAEMHW010000000
VERSION     DAEMHW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.179"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            203..736
                     /gene="pyrR"
                     /locus_tag="KPK67_003100"
     CDS             203..736
                     /gene="pyrR"
                     /locus_tag="KPK67_003100"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBG2460029.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            1020..2231
                     /locus_tag="KPK67_003101"
     CDS             1020..2231
                     /locus_tag="KPK67_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBG2460030.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKETYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            2530..4182
                     /locus_tag="KPK67_003102"
     CDS             2530..4182
                     /locus_tag="KPK67_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2460031.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYIISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNIF"
     gene            complement(4397..6172)
                     /locus_tag="KPK67_003103"
     CDS             complement(4397..6172)
                     /locus_tag="KPK67_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBG2460032.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSLILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            6468..>7400
                     /locus_tag="KPK67_003104"
     CDS             6468..>7400
                     /locus_tag="KPK67_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HBG2460033.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINK"
BASE COUNT         2778 a          814 c         1267 g         2541 t
ORIGIN      
        1 tatctttaaa cgatacagag atatcgtaag acaattgcta aattgcactg tatatttttt
       61 atataagagc ttcttgtctt gtgatgagaa gtttttttgt tgtttaaatt gaaattttaa
      121 ataaaaataa atttataaaa aattaaaaga aaggagtaga tttgtcaaat agatatatta
      181 tatctttgac aataattatg atatggtaga aaaagctcaa ttaatggatg aaaaggcaat
      241 agcaagagca attactagaa taagtcatga aataattgaa agaaataaag gtgtagaaaa
      301 tctagtttta gttggaataa agactagagg tgtgccaatt gcaaatagaa tttctaagaa
      361 aatagaacaa atagaaggaa caaaagtaga tacaggagat attgatataa ctttgtatag
      421 agatgatttg gaaaaaattc atgttgaacc tgtagttaaa ggaacttatt tagattttaa
      481 tgtaaatgat aagacagtag ttttagttga tgatgtttta tacactggaa gaactgtgag
      541 agcttcacta gatgctatta tagatatagg aagacctaaa tcaatacaat tagcagtgct
      601 agtggacaga gggcatagag agttgcctat aagagcagat tatgtaggaa aaaatgtacc
      661 tacttctaga catgaaatta tatctgtcag tttattagaa attgatggag aagattcagt
      721 tacaatcaaa gaataattat ttttaagtat tatataaaag cttagcattg aatagtcagg
      781 gaggaggata aagcttacat agattgcaca aaataaatat tataatttta tggatgtgag
      841 tcttaatgta gtttttattc tcctttattt gagtttttga tgtcgtaaaa tgtaaaataa
      901 tgataaatta tataataagt atatagaggt taaaaaatag aatctaggga atgataatta
      961 attaaaagat attaattaaa cagaggattt atttatatat aataattttg gaggttttta
     1021 tgaaaaaaac gataatgtct ttacaacatc tattagccat gtttggagcg acagtattag
     1081 ttcccatttt gacaggtttt aatccgtcag ttgcaatttt ctgtgctggt gttggtacat
     1141 tgatatttca tttttgtaca gaaggaaaag tacctgcctt tttaggttca agttttgcgt
     1201 ttataccagt tatactagca gctaaagaga cgtatggtgg agatttagca tatgctcaag
     1261 gtggtataat agtagcagga ttaatttata ttataatgtc gattatagta aaaatagttg
     1321 gtgtaaataa aattaaatta tattttcctg cacaagttac aggagctatg atagtagtta
     1381 taggattaaa tttattgcca acagctttta gtatggcttc agccaatttt gtaatagcat
     1441 ttataacttt agcaatagca attttaacca ataagtttgg tagaggattt ataaaacaat
     1501 taggaatatt aatagcagtt ttttctgggt atataatctg tttgatatta agattagtag
     1561 atataactac tattacagaa gcaagtttat ttgcaatacc taactttaca gttcctaagt
     1621 tcagtttagg agcaattgtg attatatctc cagttgtatt agctgtattt atggaacata
     1681 taggagatat gactactaat ggtgctgtag taggtaaaaa ctttatagaa aatcctggat
     1741 taaatagaac attacttggg gatggatttg cgactatagt tgcagggtgt ttaggaggac
     1801 cagcaaatac aacttatgga gaaaatacag cagtacttgc tataactaaa aactatgacc
     1861 cttcaatact tagacgtaca gcaatatttg ctatattgct tgcatgtgta ggcaagtttg
     1921 gtggatttct tcaaagtata ccaggttcag ttatgggtgg aattagtata atgctatttt
     1981 cgatgataac ttatgtagga ttaaaaacta taagggacag ttcttgtgta gaaagtaaaa
     2041 taaatatatt aatcatagca gttatattat taataggtct tggaacaaca tatttatcta
     2101 ataaaggtat ttctgttgga atccctataa caagtacagt aaagataact ggtcttagtt
     2161 tagctgcaat tgttggaatt gtattaaaca gaattttaaa taatcaggac tttaaagttg
     2221 aagaagaata aatttaaaga ataagttgta tattgaataa ctaaaattag ttaattttct
     2281 atagacattt gtaattaatg ttgatataat gattaaaaat aaatgaaaga aagggcaagg
     2341 taactaggac aatgagaata taatctattt tttataattt aaagtattgt aatttttgaa
     2401 aagcctattt tggtggcttt atttgcaatg cttaaattaa ggagagtaga ttatagtcta
     2461 tacgtttatt tgttatgcct atttatagaa tctctcctta tatttaagtg tgttaaggag
     2521 ggatttttta tgttgttagt aaaagtagag aatttaaaga aatattatgc agataaatta
     2581 attttggata tagataaact tgaaatatta gaaaatgaca aaataggtct agttggttca
     2641 aatggtcaag gaaaaacaac attattaaaa gcaatattgg gagaaataga aattgatgaa
     2701 ggatatactt accttacaga aagctattct tatataagtc agagtgaaaa taatattgaa
     2761 acatgtagtc atagtaagga gaagagtctt ttaaatgctc cagataaatt tgaagaacac
     2821 ttatcaggag gggaaaaggt taagttaaaa atagcagatg cgctgagtaa taagaaaaat
     2881 atcataatag ctgatgagcc aacttcaaac ttagataaaa aaagcattgg ggttttggaa
     2941 gatatgttta aaaggcatga gggagcatta ttattaatat ctcatgatag acgttttctg
     3001 gatgagttat gtacaactat attagaacta gaagatggaa aactaaaagc ttacaaaggc
     3061 aattacactg attatttaat gcaaaaagat gaagaagtaa aaagagctga ttttgagtat
     3121 caagaatatg ttaaagagaa aaaaaggctt gaaaaagctc ttttatataa aaaagcttta
     3181 agtgatggca taagaaaaac accaaaaaga atgggtaatt cagaagctag gttgcacaaa
     3241 atgggtggtc agactaataa gaaaaagttg gactcaaatg tgaaggctat aaaaagcaga
     3301 attgataaac ttgaggtaaa gaataaacct aaagtttcta aagaaatgaa cattaagatt
     3361 caagatggta tggagataat tagcaaaaat ctagtagagg taaaagatat gactctaaag
     3421 ttagaaaata agctcctgtt agataatgtt tcctttaaga taaaaagagg taaaaagata
     3481 gcattattag gtgataatgg gtgtggaaaa agtactctga taaaagaaat attggctgat
     3541 aaaaatgaca atataaaaat aaataacaag gtaaaagtag gttattttga ccaaaatcaa
     3601 agtttattag atgaggaaaa gagtgttcta tacaatacta aagttaatag ttcatttgat
     3661 gaatctttta taaggataaa cttaagccta tttgggttta aaggtgatga tgtctacaaa
     3721 aaagtaaaag tacttagtgg tggagaaaag gtaaaaatag cactatgtaa aataatattg
     3781 gaagataata attttttagt atttgatgaa ccaacaaatt acttagatat aaaatctatg
     3841 gaagcactgg aaaaggcact gataaatact gataaaacta tgcttatagt gtctcatgat
     3901 agagtatttg tatcacatat ttgtaactat attatagaga taaaagatgc taaaataagg
     3961 gagtttgatt gtaattatga cgaatatata attagcagaa ataaaaaaac tcctagtaga
     4021 gaaaaccaaa tcaaaaaaga aaatcttcta gtattagaaa atagacttac aactgttata
     4081 tctatgttat ctatagaaaa agataatctg aaaaaagaat tgtatgaatc agaatataat
     4141 gaattactaa aacaaatcaa taaattaaaa aatatctttt agtgttaact ttttaattac
     4201 atctatataa gaagactgaa aatatttgtt gggaatataa gtatagttat taaaacagaa
     4261 aagtattttg ttagaaaatt aaaagtaagc taattagaaa gtggccttat tattgtactt
     4321 tataagaagg ccactatttt tatttagaat gataatgtgt agttgtctaa catttatttt
     4381 gtatttagtg cagaatttat tcagatttaa ccttaagctt ggctactgtt tcttcttcag
     4441 gagtaacata aatagtactg ttagtttcat aaataaccat acctggttta gaaccactag
     4501 gttttttcac attttttctc ttagtatagt ctacaggcac ctgagaagaa agttttgact
     4561 tactaaagaa tgctgctagc atagctcctt caaatacagt attatcaggg acttccttac
     4621 cagcacactt tattattaca tgagaaccag gaatattctt tgtatgcatc cataagtcat
     4681 cattatcagc aagtcttagt gtaagatagt cattttgctt attgttttta ccaaccaata
     4741 ttttaaatcc atcagaagaa acaaattcat gaggttttgt agatgggata gtatcctttt
     4801 tactgttttt cttttgagtc ttgatgtatc caaccttagc aagttcttct tttatatctt
     4861 gtaattcagc taaattttca cagttttcaa tacttaaaat tatgttttct aagtaatcta
     4921 tttcttcctt atttaaggaa atttgatgag atatctcgac tttagcatgt ttcattttgt
     4981 tatacttttt aaagtatttt tgagcatttt cagatggtgt caagttttta tttaattcta
     5041 ttgttacatc attacaattt tcatcataga agttagcaac ttcaatactt tccatacctt
     5101 tttctaccat ataaatatat gaagtaatca attctccttt tatcttataa atgtctgcat
     5161 tttcggattc tgataattct tcttcttgtt ttttaagttt attataaagt ctatcaagct
     5221 taacagatat actcttcttt aaatcagatg aacgttgatt aattctatct ttaatatctt
     5281 tagttctata gaaatcctcc agaataagag acatactatc cttatttata tatgataaat
     5341 cgctaaataa tgtaaggttt atacaactga aatcaactac cttatccacc tttttatcta
     5401 taataatgca aggagagtat ttgttagaat ttatatcatt gaataaatta caaaatactt
     5461 tgtgtaaaga atcaaattgt tcatcagata tatcctttat gatagcattt tgatttacac
     5521 ctgctctgta gcaaatttct ttagctataa tagggcttat acctagaaat ttagaatata
     5581 tagacttaaa gataggtcct tccaattcct ctaggttttt aataaacaaa tccttagata
     5641 tatcatcaag agggttgagt ttattttgtt ctggtggtaa tgaataatca tgaccaggta
     5701 aaacttgacg aactctactt atgctaaaag ggactctttt tatagaatct ataattttat
     5761 tttcttcacc atgagttatt attatgttgc tatgtcttcc cataatttca atcataatat
     5821 ttttaactgt cttttctttt aattcatcta aagattcaac acttattttt attattcttt
     5881 caaaacctat ttgagaaatt tctactatat ttccaccttg tatgtatttt ctaagtaaca
     5941 tacaaaacat aggcgctttt agagggtttt ctttttgata attacttgta agataaactc
     6001 taggatttga ggcacttgca cttaaaacca atctaaaatt ttctttatta tttcttatat
     6061 taaaaattac ctcatcatct tcaggttgat gtatcttatc tatttttcca cctgtaagtt
     6121 ttgatgataa ttcatcgact attgaatgta taactaatcc atctaatgcc atttatagca
     6181 cccctttctt tgaccaaaat gagtcagtaa attaattata cacataatat acgctttgtg
     6241 actaggggga atttaaacaa tagagattaa tatttaaatt gtataattat ttatatttat
     6301 tagagaggaa actttatttt taaaattaat aatttaaagg agattaaata taaagtaaat
     6361 atagtataat tattgtgtag agtataagct atgaaagtat atgaacttta cagaaaaaat
     6421 caattttaag gttctatagt atataaatat gaaagaaggt tgaaaaaatg agatttatcg
     6481 atttaagaag tgatactgtt actatgccta ctgacgaaat gagagaagct atggcaaagg
     6541 ctcctgttgg agattcaatt tttagagatg accctactgt aaataaactt gaagaattag
     6601 cagcagctaa agttggaaaa gaagatgcta ttttccttcc aagtggaact tttgggaatc
     6661 aattagctct atttactcat tgcctaagag ggcaagaaat aattattggt aaaggttatc
     6721 acattgtgac tcatgaggtt ggggcacctg ctgtaatagc tggagttcag cttagaacta
     6781 tagatgaaga cgaaagtgga gctctaaatc ctgaacttgt tgaaaaggca attagaagag
     6841 atgacataca tgaaccacag acagggctta tatgtgtaga aaatgcatat tcaggtggaa
     6901 cagttgtgag tcttgataat ttaagagaaa ttaaaaaaat agctgagaaa cataacttac
     6961 cagtacattt agatggagca agattattta acgcagcatt aactttgggt gtagaagcta
     7021 aagaaatagc aaagtgttgt gatagtgtta tgttctgtgt gtcaaaaggt ttagctgcac
     7081 caatgggttc tatacttgct ggaagtaaag aatttatagc aaaagctaga agaaagcaaa
     7141 aagttatggg tgggggaatg agacaagtag gtatagttgc agcagctggt ataattgcca
     7201 ttgaaaaaat gactttaaga ttgaatgaag accatgaaaa tgcaaaatat ttagcttgtg
     7261 aattaaataa aattgatggc attgaagtaa acaatgttaa tccagatata agtatggtat
     7321 tttttaagat gagtgaagat attataaaag aggatgtttt aataaaagaa ttttttgaaa
     7381 gaaatataaa aattaataaa
//
LOCUS       DAEMHW010000180         7395 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEMHW010000180 DAEMHW010000000
VERSION     DAEMHW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.180"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(47..709)
                     /locus_tag="KPK67_003105"
                     /pseudo
     CDS             complement(47..709)
                     /locus_tag="KPK67_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(869..1405)
                     /locus_tag="KPK67_003106"
     CDS             complement(869..1405)
                     /locus_tag="KPK67_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG2460034.1"
                     /translation="MIGLKCGIVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1478..1891)
                     /locus_tag="KPK67_003107"
     CDS             complement(1478..1891)
                     /locus_tag="KPK67_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG2460035.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2357..2995
                     /locus_tag="KPK67_003108"
     CDS             2357..2995
                     /locus_tag="KPK67_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBG2460036.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEDTI
                     SGMQGTISDMQGSISSMNGTISDMQGSISDMDGTINSMQGSISGMDGTISDMQGSISS
                     MDGTINSMQDSITDMQVEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIK
                     SDVKINRNTLDTVIKHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3602..3781)
                     /locus_tag="KPK67_003109"
     CDS             complement(3602..3781)
                     /locus_tag="KPK67_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2460037.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDKSYDIVFSCIVFH
                     HYTNVQNIFCIFWRW"
     gene            complement(4007..4540)
                     /locus_tag="KPK67_003110"
     CDS             complement(4007..4540)
                     /locus_tag="KPK67_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG2460038.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQKHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(6060..6632)
                     /locus_tag="KPK67_003111"
     CDS             complement(6060..6632)
                     /locus_tag="KPK67_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG2460039.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGTNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(6694..6957)
                     /locus_tag="KPK67_003112"
     CDS             complement(6694..6957)
                     /locus_tag="KPK67_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG2460040.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(6957..7178)
                     /locus_tag="KPK67_003113"
     CDS             complement(6957..7178)
                     /locus_tag="KPK67_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG2460041.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         2599 a         1247 c          888 g         2661 t
ORIGIN      
        1 ctactaactc atgactaaag tcacaagtgt tcgtagctat tctttaatca aactggaata
       61 cagactccat tcctaggaaa ttggctgaaa tatagtctgc tgtcaaagat gctgtcatca
      121 cagaaattaa aacagatact gaaaagtttt tatggacttc ttcaagtgaa aacataacac
      181 ccgccagtgg tgcatgaaag gctgctgata atccagcact tgccccacag gttaacagga
      241 atttttcctc tgtcttccct cggtcaagag ctctagaaat acctttacct gccatagcac
      301 caagctgaat ggaaggacct tcccttccca aagcaagacc acccataagt gataagaatc
      361 ctccaataaa ttttgttggc aacactcgcc accatacttg ttttattttc cctgtcattt
      421 ccccctcaag ctgagggata ccacttccag aaatcattgg ctcccagtcc accaactttg
      481 atacaatcca cgcaagtact gcaagtactg caaaccaagc tattattttt attgtagaac
      541 cttctacaaa ttttaaaatt tggctaagcc aagtaccagc ctgccctaaa agtatcctgt
      601 ataaaagcac aatcaaacca gccacagccc caaccataag tccttcacca atcaaaatta
      661 cctgaaagtg ctctgcccgt ttgataatat gagatgtatc ctttttcata ctgttcctcc
      721 ttcatccaat aattctacac aatctgaatc tccaatgact aaagtcatga gtgtgtacag
      781 atatttttta atcaacaaaa atagattttt atttactaaa tataagaaaa taattgctca
      841 atttctcaat aaatgtgaat ttattctttt aggtatttat tctccacagt cgtgcttctt
      901 ccagcaaaat atttattaat tcttgctttc tctgtgtata gattttacga ttgtcctcaa
      961 actgtaatgc taatttttgt ttatgactat cataaagttt tgctttgtct gtaaaagtat
     1021 ttagataatc tcgaaaattg atataattat tccatgcaac actattccat tcaacaacat
     1081 gaatatggtg agtacgagta tttttctcaa aatctcccat agcaaaaagt aattgttttg
     1141 gtacatcctg acctcgaaaa acgattccac attgctctaa cctttcgagg tataatttag
     1201 cttcctctaa agaagcaaca cctacaacaa tatcaataat aggctttgcg ctaatactgg
     1261 gaatagaagt gcttccaatg tgttgaatat caatggcagt cttatcccat atacttttca
     1321 ataatataat gacatctttt gcacttttat cccatatcat ctgatgttcc aataatttaa
     1381 caataccaca ttttaaacct atcactatat accacctcaa tataatccac ctttatatat
     1441 attacaattt tttctatctt gataaacagt gatttagcta tttcactttt tcttgacact
     1501 cgctacattc tctatcatga atagatataa ttcctccaca tttagaacaa gtatatctct
     1561 ccttctgctt ttgcataaac ttctctaaac cgtactgctg aacaaattta ctattttcca
     1621 taaggcttgc ttgatatctc ttgttataac ttttttcaag attttttatg agtttacaag
     1681 gaaaattgaa acattcaaaa caataaaaca accctttttg acagacacaa tcctttattt
     1741 tacacttacg gcagtgttct ggctttcctg tatcgccgtt caaacaacca gcacatggct
     1801 ttttgtgata acaatgctta tagcaaacca tacaattcat tccacaagga gcaaacataa
     1861 tcgtatcaat actctctttt ggcattttca taacatttta ctcctttcaa aaatccaatt
     1921 tgtcgaattg ttatatattc atattgtaac ataaaaaata gctaaaaaat tcatgcactt
     1981 tcattgccaa taaattttca aggaaatatg aaagtttaaa actaagaata aaaagagata
     2041 aaaaggaagc tactaataaa aatattcaaa cacaagtaat caatataaat aacttaaggt
     2101 atatttaaaa gtatatgata ttaccagccg ttttaaatac aaatataacc acacatgcct
     2161 caaaaacact tattacagta tcttagaaac ttagacaata cataagattc attaataaaa
     2221 aagttctcaa tatagatata tttaaccatg ttttgaatag cagataatta tatgattaaa
     2281 aattaaatat gacatagcgt ttgtggaata taacacctaa attatgttat aataataaaa
     2341 caggagtgtg atagtgatgg atgaaaaaat attagagtta ttacaggaaa tgcaaggtaa
     2401 tattagtaat atgcaaggta gtatcagtgg tatcgaaggc actattagtg atatgcaaag
     2461 caatattaat ggtatcgaag acactattag tggcatgcaa ggcactatta gcgatatgca
     2521 aggcagtatc agtagtatga atggcactat tagcgatatg caaggcagta tcagtgatat
     2581 ggatggcact attaatagta tgcaaggcag tatcagtggt atggatggca ctattagcga
     2641 tatgcaaggc agtatcagta gtatggatgg cactattaat agtatgcaag acagtattac
     2701 tgatatgcaa gttgaaatta aagaagtcaa tagtaggtta gacaatatgg aacctgatat
     2761 taaagactta aaaactggac aagttgaaac tagtagtaga cttgatacta ttcagagtga
     2821 tgttacagaa attaagtcag atgtaaaaat taacagaaat acattagata cagtgattaa
     2881 acatacctct tcattagtaa aagatacatc agatattaaa aatgatttaa ataatataga
     2941 gatagtcaca tctaagaatt cgaatgatat tgctaaatta aaatcagtta aataatatac
     3001 catagtaaaa aatacctgaa aatttcacat acttctttaa agttatacca aattttatac
     3061 ataaatactg attatccgac aacacacttt acttataaat aatttattaa aaaaatactc
     3121 attttatcct aaaattaaca cgactaaatg agtcttgata taataataaa aaataaggga
     3181 gattggacac taaaaagttg tgtatctccc ttatattcta taatatagtt atataatttt
     3241 tcagcctaaa ataaaagtcc atctttgatt aaaacacatc cccttatcac ttataaaagg
     3301 ataaaatctt gttttcattt ttccatttat atcttcttgc ttaagagtaa taattttttc
     3361 ttttgatact ttaagtgttt ggttaaattt aaccaatcga ctaagttttg ttcaacatct
     3421 atttttcata ttttccaata cttaatctat ttttataaat aattctttta tttctaactc
     3481 acttacattt tttacataaa acgaagcata taaatttcaa ccttatcttg tatagttcct
     3541 aatacttaat gagtagctta agtatcaatc attataaatt ttctccttac atttaacact
     3601 cttaccatct ccaaaatata caaaatatat tttgaacatt tgtataatga tgaaatacaa
     3661 tacatgaaaa tacaatatca taactcttgt ctaaaaatag aagttcttca acataacctt
     3721 tcacaaaaaa catattatct aaatattttt tgtctgctcc ttgttttcct gtttgtagca
     3781 tataaattac tgcatctaaa catataacta ctcacacgaa aaaagaaaat aaacttccaa
     3841 aatacaagct ctaaaaacta cctctaaagt tgtataatgt ttactaggaa taaggttgta
     3901 taacatatag tttaaataat cattttttaa aattgcaagt tattgctttc aaaattagat
     3961 gtttgtatat caaataaatg attaagaatt tctaaattta atggctttat aatgcactcc
     4021 ctttctttgg gataaaataa tgttgcacag atactttttc taaagataac aattttattt
     4081 ttctatcaat tccaccagca taccctgtca aactcccatt tgttcctaca acacgatgac
     4141 atggaacaat aatagagatt ccattatgcc ctactgcacc accgattgct tgtgctgaca
     4201 ttttagatat tcctttttgt tgagcaactt tatatgcaat ttctccataa gtgattgttt
     4261 taccatatgg tatttgttgt aaaattttcc acactgtagt ctgaaaagga gtacctacca
     4321 tatggagtga tggtgtaaaa ttgggttctt ttcccgaaaa ataaatatct aaccatttct
     4381 ttgtctgctc gaaaattggc aattcttttt cttgatgttt ttggtcaaga ttataagcat
     4441 aatacttttg attctcaaac caaagaccag ttaacccaat attatctgat gctaaaagaa
     4501 tatttccaat tggactatgg taatagctaa tatattgcat acaattcctc cttatattct
     4561 gaaaacttca ctcaaatcat tagacacttc ttttctatct gccattatgt aaaaacatac
     4621 taatataatt tccttctaac tacactttcg ttataatatc tatcctcata tcatattagt
     4681 tttatattta cctgcatctg ttcatccttt tttccctttc cctttgatga atataaaatt
     4741 catacattta agcttcctac tgtattaact tttatagaag tgtatattat aattttatca
     4801 ctaaatagac caaaaaaata tcgatataac atcaagtaat atcataatac tttcaaatgg
     4861 gaaaagtaag aataggctgt aaaaaagaaa agtattttta tgttagaaat ccagtaaaat
     4921 cgttagtaga tagaataaca gaaaacgaac aattcgctat ttatcatttt acacataaat
     4981 ataattggcg caataaagtg ttttctattc tacaacgatt aataaggtta gaaaaaagta
     5041 agaatgaact gtatatgtat gaagcattag tttctgtttc tttcctttgg tttattatga
     5101 taaaaaatat tgtttttcca ccaataaaac acaaaaatat aattaatatc catatgcaaa
     5161 aaattttacg ttatattgaa aaatactatt gttttacatt atattgaaga gtactattgt
     5221 gaaaacttaa ttttggagta ctatcaagca gtgataatat caataaatca gagtgttcca
     5281 gaggatttga actaagttta aatactatac cttgtaaata ttttactgag ttttgtttat
     5341 taaaagcaat tcaatttcta aaaggcacaa atgagctaat aagtgacatt actataaaag
     5401 ttgtttttca tcaaataatc tattttagta aatgttttaa agaaaaaacg ggatattctc
     5461 caaaagtgta cagaaatata aaaaaagaga tatgattttt tatttctttt gattatccaa
     5521 agagtaaata aaaaatattg taaaaaactt actaacaacg gcaatgcttt gaactatggt
     5581 aatactttga acttttgatt ggactttttt tgctaaaata atatatttaa atagcaaagt
     5641 aatctgttta atcaataaaa aataagagat aaaagaaatc aactaaaact tttacctctt
     5701 attttgatag tttatcagag ctaatattta cactcttttt atttttgata cttcatttta
     5761 tttttgatac ttcattaaaa atgatgctta ttctcttttc atttttaata cttcattaga
     5821 acttatgctt attctctttt cacttttaat tcttcattag aacttatgct tactctccat
     5881 tcacttttaa ttcttcatta ggactatgct tattctccat tcacttttaa ttcttcatta
     5941 ggactatgct tattctccat tcacttttaa ttcttcatta aaaatgatgc ttactctctg
     6001 ttcactttta attcttcatt aaaaatgatg cttattctcc attcactttt aattcttcat
     6061 taaaactgat gcttgcactc tgtaaactca taaacatcca ttaatgcttc accaaatctt
     6121 tgatagtgaa ctacttccct ctcacccaaa aatctcaata tatctttcaa atcagggtca
     6181 tcagttaaat ttattaattg atagtacgta gccaatgctt tttgttcagc agccatatcc
     6241 tcatgtaaat cagttaccga atcgccaaag acattaatat aacttgtagt aaagtttact
     6301 ccattagaat ctactggctg aagaccatgt ccaaaattag cataattact accaagtcca
     6361 gcagccttaa gttcttctgg agtacaattg cctattaatt gatataacac tgaagaaatt
     6421 atctcaacat gagccatttc ctctgtacct atatcagtta aaagtgcacg agattttcca
     6481 gttggcatag tatatctttg ttgaagatat ctagttgcag ctgccaactc accattagtt
     6541 cctcccaact gagtcatgat atattttgcc attctagggt ctgcttgtct tatgttaact
     6601 ggatgttcca gtgttttttg ataaatccac atattatctt actcctcctt aatatatatt
     6661 taataataaa tctaatttta aagtttaata attttattta taaaatgatt tgtcccatgg
     6721 ccaaggttca gatatccatg accaaggata ttcacttgat tcatatccaa aattagttaa
     6781 tgctccatat ttagattcat aatcacacac agcttgtgta aatttatcgc agtatctgtt
     6841 atatgaatca agagctttct tatcccttgg attgttatct aaatataaat ttaattctat
     6901 acaggcaaat tgatattctg aaattctctc taaaagttct cttctattta atgacattag
     6961 tacatgttac cccctttttt agctctaact ctatttttag gatttgcata tagccaatca
     7021 ttatagttat ttgactctat taaatctaat tcaggaaata aagtacctct ttttaatgct
     7081 gtttttggat tgtatgtgtc atcataaatt tgacgattga tgtatggtct agcaaattca
     7141 caacaatagt cttgtttatc ctcttttaaa tatcccatta attatcctcc ttaattttta
     7201 gaattttaaa atagcattat ttttaaaaat tgtatactgg ttctatatta taactggtag
     7261 ttttaatagg tctttaataa gattaaatga ttaaataagt atcttttttt cttattttac
     7321 ctctaaaatt tttctaatta atctttcata aatatctatt ttaaataaat aatatagagt
     7381 ttatacaatt tttta
//
LOCUS       DAEMHW010000181         7237 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEMHW010000181 DAEMHW010000000
VERSION     DAEMHW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.181"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(226..1476)
                     /gene="cme"
                     /locus_tag="KPK67_003114"
     CDS             complement(226..1476)
                     /gene="cme"
                     /locus_tag="KPK67_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBG2460042.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(1562..2308)
                     /locus_tag="KPK67_003115"
     CDS             complement(1562..2308)
                     /locus_tag="KPK67_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2460043.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(2442..3464)
                     /locus_tag="KPK67_003116"
     CDS             complement(2442..3464)
                     /locus_tag="KPK67_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBG2460044.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGAGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(3464..5884)
                     /locus_tag="KPK67_003117"
     CDS             complement(3464..5884)
                     /locus_tag="KPK67_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG2460045.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLEQGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVSNGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(5970..6890)
                     /locus_tag="KPK67_003118"
     CDS             complement(5970..6890)
                     /locus_tag="KPK67_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2460046.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         2404 a         1165 c         1044 g         2624 t
ORIGIN      
        1 ataacctaca agtaaaggta ttattatata aagttaataa agatagtatc aaaatattat
       61 ttgctataaa gagagggact caaaataaag ataaacctat ttttcaatgt tgaaatgata
      121 aaatatattt gatttcatat aaaaaatttt cttcataaat taaaaagtcc attttaagcc
      181 ccctcatttt taaccagata aatcgagtta aactatttaa atatattact cggattcatt
      241 tgacttagat attccttgct tgtaaaattg ttttgaatag gggatagcca aagcaaaaaa
      301 taaaagtata atccctgcta tgattattaa attattaata gaaattacat ctgccattgg
      361 tccgaaaatc atcataccaa gaggaataaa accagtatat ataataatag gtaagctaag
      421 aactcgacct agcatttgtg gtgctacatg ctcttgcaac atagtcatag ctgatgcttg
      481 aatgattgga attgaaaaat tcgtaaaaaa cataaatata aaaaattgcc aagcttgtgt
      541 tacaagacct agaataattg aaaaaatagc ataggataat attgctagaa aaaaggtctt
      601 atttctattt ttaaaaccac caaaaactcc tataatcaat ccacctaaaa ccatacctaa
      661 aaatccaatc atttcaataa tagacaggta tatataaccc ttgccaaatg ttcgttcaat
      721 aagaagtcct ataagaaatc ctgatggaac acaaagaagc atgaatgctc cataagtgaa
      781 aagcactttt ctaatgaact tatctgaaaa acagtacttt atccctgata ccatatctgc
      841 aaaaaatgag actttttcag tttgttttgg tatatcatgt ttaggaattt tgatgaatat
      901 caataataca atagcaataa ttgcagtaaa tacatcaatt aaaagaactt gatttatttt
      961 attaccaaat ccaagtattg cagcagctac aataggaact ataaattgta ctatagactg
     1021 tagactacta tttataccat taaaacgcat taaatgttct tctggtacaa gttgaggaat
     1081 cattgcgttt acagcaggtg tttgtacacc acttcctagt gaacgtataa cagatattac
     1141 tgatatggct attaattcac ttgttgtgcc atttgatgac atcataaata gaattagaac
     1201 taatgtagat atagcaattg ttgaatctga tacaattatg agtacctttc gattgtagta
     1261 gtctgcccat acaccagcaa aaaaagaaat tatcatttga ggtataaagg agcatacagt
     1321 taaaactgtt acccatacac ctgatgatgt ctgaagtgtg acataccata tcattgccat
     1381 ttgtacaagt gatgagccaa ataaggaaat atattgactt gctaaaaaaa gaatgacttt
     1441 tttcttccat gtagaattag tatttttgtt ttccatccta ttcctccttt gatttgtgtg
     1501 ttgaattttt cttgctctta aggataacac acaaatcaaa gtctggagtt agagtggagg
     1561 gctaggaaaa ttgttttttc ataatttgaa gatgagattt catggttttt gcattatgtt
     1621 cagcttcttt caatgaagtt atcagtttgt cacacactga gataatttcg tcatcatctt
     1681 ttggagtgtc aaggacagtt ttaatattag tattaggatt agaagataaa gcatttaaca
     1741 ttcgtaaaat agctgaaaga gaatagtttg cacaacgaag tgcgcttata attttaagtc
     1801 gttttatatc ttcatcagta tatacacgat aaccattttg ttttcttttt acttttaaca
     1861 aaccattcat ttcccaattt cttaaagtat ctattgtaac atttaagtgt tctgcagttt
     1921 cttttctagt taaaaagagt ttatttgttt ctggagtatt tccatttaaa atctgtgcaa
     1981 caatagtaag tgcttcctcg gcattagatt tttctatttc taattgttgc aagtattgat
     2041 ttacaagact aatagcctta tcaaagtccc tattcgctga tgttcttaca atctcaaggg
     2101 catcttttct taatcccctt tgtaccactt caattttcaa ggctgtacga gcaaatctaa
     2161 attgttctat atgaaaatca ttaaagatac gataaccatt ggtattacgc ttaggtttag
     2221 gtattaagtt tagttcttca tataaacgta ctgtattagg atgtacacca attaaatctg
     2281 ctacctctga tgtcgtgtaa gtgtccatta gtattcaact ccttttagat atatagtatt
     2341 agtcataata acaccaaaca aaagtctggt gttataatgg agggtttggc tatctaagag
     2401 taattagttt atgaaagagt aattaattta tgaaagagta attaatctat tatagaagta
     2461 agtatatatt tttcaataaa ctcccatcta tcacgaggtt taaacactat tttagatggt
     2521 atggctatta ctaagtcata ttgtggaata caacaaatag catttcctcc agctcccatt
     2581 gctgaatacc acaaaacacc atctttttta catagccacc acagataacc gtattcatta
     2641 ctattcattt taactgattc ttcaatccac gatttagata taatacgttt attattccaa
     2701 ataccatcat ttaaatacag aaaaccaaat cgcaacatat ctctagcact caatgtaagt
     2761 ccccagccac ctatagaata accctgtcta tcttttgccc aacccttaag attttttcca
     2821 aagaaatctt caaaaccaaa atctttcata tcataatctg gtataacttt cataccaata
     2881 ggtttaaata agtattcatt agcaaattca cgagcagatt ttccagtagt ttttgttata
     2941 atagctgaaa gtaggtgcgt tccagctgaa gaatatttga aagtaccaat ttcgcctttt
     3001 tgaccaagtt gattcaaggt atattttatc caatcttttt gtttgcaaag tctttctaaa
     3061 gactcagtca tatttttaaa agggtatggt gcagtcattg taagtaggtg acgtatagtt
     3121 actgcttttt tacatatttc agaaggacta caagtatatt cagggaaaaa atcaacaacc
     3181 ttttggtcaa catcttttat atatccttta tctattgcaa tacctattaa ggctgatata
     3241 atactttttg taactgatgc aacatgaaaa gtatcatcag cattatatcc attatagtac
     3301 ttttcaaaga caacttttcc ttttcttgca ataattattc catttatatt actatattgt
     3361 gagattatta aaggttcaag ttgtgaaagc ttttctgaac tcatgccaac acttgaaggg
     3421 tctacttttt caaatattaa atctaagcaa ttgtttttat tcattaaaga tacctccctg
     3481 ttatagattt tggattttct tttagagttt ttggagaacc ttctgcaatt atataacctc
     3541 ctttgtcacc accttctgga ccaagctcaa caatccagtc acaaacattt aatacatcta
     3601 tatcatgttc aacaacaatt acagaatttc catttgatac tagttcatct aataattgaa
     3661 tcaacttaga agtatcatat agacttagac cagtagttgg ctcatccaat acataaagga
     3721 tattaccatt tcgtttttta ccaatttctt ttgcaagctt gattctttgg gattcaccac
     3781 cacttaaagt cggtgtaggt tggcctaatt ttatataccc catacctatt tgttctaata
     3841 catttaagtg tgaaacaata tttggaatat ccttgaagaa atgtacagat tcagaaacac
     3901 tcatatttaa aatatcatgt atatttttat ttttatattt aatagataaa gattcatcat
     3961 taaatctttt tccctgacat tcactgcata tatggtctat acttagatta tttcccaacc
     4021 agaccttttc atatccacta ccactacatt taggacaggc acctttggag ttaaatgaga
     4081 aatgaccagc tgtaaatcct tgttctaacg attctggctg acctgcaaat agtgtacgta
     4141 tcttatccca tataccaata taagaagcag gagttgagtt catatttttg cctataggag
     4201 attgagatat ctctgaatat ccagaaatat gtttagtacc aatcagtcta tctgcaatag
     4261 tttcaacaac tgcataatcg tcttcctcac ctatttcact ctctgaagaa tttatttgat
     4321 tattgatagt atggttatta aattggcttc taagaagagg aagtagggtg tctgatataa
     4381 gtgaactctt gccactacct gattttccag atattccaac catagcaccc aaaggtaatg
     4441 atacagttac atttttaaga ttatttgtat ttgcattttg aatagttata caaggtattt
     4501 tatcagtatg tgtaatgttt ttattgcttg ttctattagg cattggatat tttccagata
     4561 gatactgtcc agtaagtgat ttgtcggatt gaagtagacc ttctaaatct ccttgatata
     4621 ctaattgacc accttcaaca ccagcttttg gaccaatatc aataatatgt tctgccattt
     4681 taattgtgtt tttatcatgc tcaactacta tgaccgtatt gcctaaatct ttaagtttct
     4741 ttattgattt taagatgtct attttttctg attcatgcaa tccagctgtt ggttcatcaa
     4801 gcacataaat taatgagtcc atctctgaat cgagatgtga atttaaaaat agtctttgca
     4861 tttccccacc actcaaagtt gacatttctc ggtataagga tagatgacct aatcgtgatt
     4921 ctattaaatg ttgagttttg gataaaatat catttacgag atttgtacca aatgtgttta
     4981 aattaccttg atttattaag agtttaagga aatcatcaat ttctaaaatc gtcatttttc
     5041 caagttctcc aatatgtttt ccatttaaaa gtactctacg tgcttcttca ccaattttaa
     5101 aaccattgca atctgaacag gcagtcatag tataaactcc atttaaatct tcgccccttt
     5161 tgtaacgatt gtgaaatacc tttgataaag aggaactttt tttaccattt tcataatgac
     5221 catagagtac ttcatttctt atttcttcag gtagttgtaa gaattgtatg tctaaataat
     5281 cgttgaattt tttagaaaat actctcaaat atcctggtgt aatctttgct ctatctaaga
     5341 cttgttttag ggtatcatgc ttatcaagga ctaatttttc aatattaatc tcaaaatatg
     5401 caccatgtcc agagcacttc atacacattc catctgaaga gttgtatgag aaataacttg
     5461 gtcttaatcg ttcttcctca tatccacaat tattgcaaat tagattatcg cttacaggag
     5521 tatcacacat agtacacatt atttgacctt ccactgaata caatagtgta agcatgttga
     5581 gaatattggt tcttgtgcct actgttgaac gaggattgct ctggcgaata atattttgtt
     5641 gaacagctat agtgggagct aaaccttgaa tattatcaaa cttatcctca ctatcaatac
     5701 ctgcaagtat acctaaagat tgcagatatt gtttacgccc ttcctcaaat acaatgtcaa
     5761 acataaggct agatttacct gagccactga cacctgttgc aacaacaagc ttatctttgg
     5821 ggatagaaac atctatattt ttaagattgt gtatctttgc tccattaatt tttatttctt
     5881 tcattggttc acctcatttt atcaatttta tcaaaccgta aaatagggat atattaatcc
     5941 ttaatacaag aatagattag gtgaggttat taaaaaggtt ctataggtaa atatatatcg
     6001 accaagtgtc tattttgagg gtgattgtgt gggttgttaa gatatacttc aaaagggaag
     6061 gaggcacgta gcttataatt accatttgat agccattcat tatacataaa atcccacgca
     6121 tcactatatt cagattttga aatatcaaaa tgtcccacaa gatattttcc agaaggtatt
     6181 atcatattgc caagattgtc attctctttg acaacaatgt tatctggaat ggtcatgcaa
     6241 atactagtcc ttaattgatg ctcctgtgta aattgtggat tatcatgata aatggctaat
     6301 atttgtgtgt tttcaagtcc aagtagattc tgctcacttg catatgtgaa taaccgttct
     6361 aaaagttttg ggaaagtact ggttagatta ccatatgaac cagtatatct agtgtaaata
     6421 gtattcatat tatcaagttc tagaatttca atttcacctt taacctctct agtattctta
     6481 catttaggtt ttgatatgct tctattgtac tgagaaattt tatatggttc tttgcaattc
     6541 ttgctataat tgtttctata ttttataggg cttattttat agtaattttt aaatgctctc
     6601 gagaaaactg ctgaatcagt aaagccaaag tagtgtgcaa tatcagtaac agtcatgtca
     6661 gttctatgaa taagaaaagc agttgcattt tctagtttta gcctatttac atactgtgcc
     6721 aaagtttctt tttctattgc tttgaagatt ctatgaaaat gaaattttga aaaacctgct
     6781 atattagaaa gttcatcaag agaaagaggt tcatgtaaat gttcttctat atagtcttgc
     6841 actttgcaaa tacgatttag atattctttt ttacttttag catttgtcat atatagccct
     6901 ccttattgtg taaaatgtgt atcaattaat ttatagcaat atagtaataa aattttatgt
     6961 ttttgtaatt tatgataaat aagatagcgt ttaatttttt ataattgata aaagatattt
     7021 ataattaata aaagatataa gatgccttat atttgtcttt gcatataagt atagtcttcg
     7081 taataattag tataccatag tgatttattt ttaattgtgt atttatataa atacacaact
     7141 tatgatatgg acgaattatt atgcaaataa gctgtaatta aataaattgc aatataaacc
     7201 agttgaaaca taagttaggg aaatattatc aaagtta
//
LOCUS       DAEMHW010000182         7189 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEMHW010000182 DAEMHW010000000
VERSION     DAEMHW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.182"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            289..1692
                     /locus_tag="KPK67_003119"
     CDS             289..1692
                     /locus_tag="KPK67_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2460047.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYSYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHKSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            1911..4388
                     /locus_tag="KPK67_003120"
     CDS             1911..4388
                     /locus_tag="KPK67_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2460048.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLIYIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGGSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTTPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMYGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDVVMIQIRDLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISIKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            4403..5089
                     /locus_tag="KPK67_003121"
     CDS             4403..5089
                     /locus_tag="KPK67_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460049.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQIMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            5363..6196
                     /locus_tag="KPK67_003122"
     CDS             5363..6196
                     /locus_tag="KPK67_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2460050.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCMQLKNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYQANMSDFYSEIAK
                     F"
     gene            6234..6695
                     /locus_tag="KPK67_003123"
     CDS             6234..6695
                     /locus_tag="KPK67_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG2460051.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            6726..6974
                     /locus_tag="KPK67_003124"
     CDS             6726..6974
                     /locus_tag="KPK67_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2460052.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
BASE COUNT         2775 a          768 c         1100 g         2546 t
ORIGIN      
        1 taaagtatcc cctataaaga tggttctatg aaaattttct ctatactgtt taacgtatat
       61 agagattttg tgtataatta taaggtattt aatggaaaaa tttatcttac ttttatatgt
      121 tatcaaaagg atttattaaa tacaacagac agcttgataa catataatta tataaatata
      181 atttatgtag atagttgtat ttttaaataa atttttacat atcaataatt attgtctatt
      241 ttatataagc aacaatcaaa ataaaagttt aagaaaagag ttgattaaat gcataaacct
      301 agttataaaa cagtatttca ttcatacatt atttttttca tgatttttat ttgcttaatc
      361 ctgacagtaa taggtatttt cttatcactt attactattc agaaaccaaa tggggaattt
      421 attcgtagtg actggccgaa aaaatttaca gaaaacttta atgaacaaat cgtatttata
      481 gataataagc cacaagtaaa acagtctgga ttagaaaaac ttcgagaaaa tcaattgtgg
      541 ttgcaaattt tagattctag tggaaataga gtttatgggt ttttagaacc aaaagagcat
      601 aaagattatt attctaattc tgaattatta gcattagcta accaaaataa ttcaagtaat
      661 ggaactgtat actttaatac aattaaaaat aataaaaatg actatattta tattatatat
      721 tttccattaa atatatctaa agtgaatatg ttttttgatg gaggtaaatt tataggtggt
      781 aagtcaattg tattttcaat catagggatt atatttttaa taattttggt ttctggaatt
      841 gcttatagtt actgggtaac aaagtgcata tcaaatatta catctgctgt tagcgatatt
      901 tcaaaacgaa gctatcttcc agttaatatt aaaggtgttt ttgctgatat atataatagt
      961 ttaaatattt taaatgaaga aattcataaa agtgatgaga tacaaaataa aactgatatt
     1021 atgagagaag agtggatttc aaatataact catgatttaa aaactccact ttcaccaata
     1081 aaagggtatg cagagcttct tacagatagt agtactaatt attcacctga aaaagtatat
     1141 aaatatgctt caatcatgtt aaagaacata gaatatacaa atacacttat aaacgattta
     1201 aaattaacct atcaattaaa aaatggaata atccctttaa ataaaaataa ttatgattta
     1261 gtaagatttt tgaaagaatt atccattgat attttaaatt atccagaata tgaatcaaat
     1321 atagttgaat ttaagagtaa tatagataat attaactttg attttgattg tacactatta
     1381 aaacgagctt ttagtaatct tattgtcaat gcttttacat atggagataa aaatactaaa
     1441 gtttgtgtac atataacatt aaatgaaaga attgaaatca ttatatctga taatggaaaa
     1501 gggatgtcaa gagaagaagc atcaaatttg tttaatcgtt attatagagg aagcaataca
     1561 aaacaaaaaa tagaaggaac tggtttagga ctagcaataa cgaagcaaat tattgaagtt
     1621 catggaggga atatatctgt tgaaagtata cctggattag gtagctcatt ttatatcacc
     1681 tttcctattt aattaaggtt aattaaggtt aaataaaaaa tatattaagg ttgatagatt
     1741 attatattta tatagtagtt tatcaatctt ttttatttaa ggggtgattt gattaaaaat
     1801 attataaata gaatgataaa agtatggagg agtctattag agatttaatt tagattaaag
     1861 tataaaaata ttagattgaa atatcttaga taagggaaga aggtgtaagt ttgaaaatta
     1921 tattaaagta tattttcaca aatattaaag aacgaaaaat tagaacagca gttatgttat
     1981 tgtccatagt tttatcaaca gttttacttt ttgtttcatt ctcaattgga ttatcttatg
     2041 aaagtgcaca aagaaaaatg gcaaaaggta tgtatggtac agcgactata tctgtccaaa
     2101 gtaaaaatcc agatatatta accaatttaa tatacatacc tgatttaaat gctataaaat
     2161 caaaggttgg tgtattggaa agctctgcta tttataataa agggggatac tatgaagaat
     2221 ttagtcttat atcagcagac ttaagtcaac taaataaaat caacaagcca cgattagaaa
     2281 atggcggtag tattactgat ttttctggtg ataaaattat tcttccaaat cgctttacat
     2341 caaaatataa aataaaaaaa ggtgattcta ttactttaca gatttatgga aagtcctata
     2401 cttttcaagt tagtgatatt gcatcttatg atacagtatt tttgaggaat acaagaggtg
     2461 taaatgcact actacccaaa gagactctat caaaaattat aaataaaggc agtggatata
     2521 caagagttct tatagagtca gaagaagaaa ttacagaaaa tctagtaaat aaattatccg
     2581 aagaactttc tactgaaaaa tatatggtat caaatactat aaatgaaact aagataattt
     2641 cagatgctag acaaaagaca acgcctttct ttttaataag tttttttgca ttaaccctta
     2701 gtatatttat aatatacagc agttataagg ttattacatt agaacgtctt ccttttattg
     2761 gaacatttag aagtattggt gccaatgaaa aaactgtaac tcgtatattg atgcttgaaa
     2821 gtatattata cggaagtata ggaggattaa ttgctattcc tattggtgta gttgttttaa
     2881 atttgatgct tcatgggtta ggaaattctc tcgaacaagg aatatcaatt ccagtagtaa
     2941 tatcacccat tggagtgatt atatctgtta tagtagcaat aatcgtttct tcatttagtg
     3001 catatatacc tgttaaaaaa gctagccatt tgccaataaa aaatattgta ctaggaacag
     3061 tagaagaaaa gaatgtttca aatcgttcta tactttttat aggaagtata atgtttattt
     3121 tatctatttt acttccaaga atatcccctg aaaatacatt gtatctagct ggtggatttt
     3181 cattactagg attaattgta gctactattg tgttgattcc tcttattact gatatcatgt
     3241 ctatagtttt tgagtttgtt tataaaaata ttttagggaa tgagggtaaa ttagctgcta
     3301 gaaatatgaa aaataacaaa aatataatac agaatatcac tttactattt ataagtattt
     3361 ctgcagttat tgcaataagt gttgttggta actttgtcaa aacttatata acagatgtgt
     3421 ttcgtgacgc agaattacaa ggttttgcag atggaaagat gaatgaagag tttattgaag
     3481 atgttagaca tatgtatgga attaaaaaaa tattacctct ttatgttatg aataatgaga
     3541 tttctggtaa tggtgtaaca ttatcaagac tagagggtac agataatata aaactatata
     3601 actctatgtt tggaatcaat tatacaaatt ttgaaataaa aaaacaagta atagaagctt
     3661 ttaatgataa acgttctgtt attttgaatg aagatactct aaaaaaagta ggcttatcta
     3721 taggagatac aataactcta tctaatgata agtatgattt ttcttataaa atagtaggta
     3781 gttttaaatc ccgtgcaaat gatgtagaag ctgttatacc atcacattat gcagttaatg
     3841 attttgataa aactaattat ggttttttag tatatacagc agtcaatcca gatgttgtta
     3901 tgattcagat aagagattta tttggagata catataattg gagtcgtaca gttgaagaat
     3961 ttaataatga ttctttaaat actataagta gttttttgtc tcctatgaat aagatgacct
     4021 atttcatttt tcttcttgct acagtaggta ttattaataa tctactgatt aattatattc
     4081 aaaaaagacg ttctatagct atgtataaat ctataggact aagtaataaa caaaatataa
     4141 aagtgacatt gattgaaggg tttacttcag gtttacttgg agctgttatt ggtattgtga
     4201 tatctatact tgagattcaa actatcttta tagttgcagg accaaaaatt tcaattaaac
     4261 cagatttaga ttttaaaaca tttattattg ttgggttatt gggaattata gtaactttaa
     4321 taggttcgat agtgcctatt ataaaaggga agaaaatgaa actgatagaa gaaattaagt
     4381 ttgaataatt atggaggtta atatgaaaat tacacataat agtttagctg tagaagcaaa
     4441 aaatattatc aaagaatata aaattggaaa tacaacaact cgagtgttaa aagaggtatc
     4501 tttacaaata atgaaaggtg aatttgtttc tatcatgggt caatctggtt ctggtaaaag
     4561 tacgctttta tatattttag gtggtttaga tactccaacg agcggaaaag tgtatatgaa
     4621 tggagctgat atatcacatt ttaatgatga gaaaatgagc ataatacgtc gtagaaatat
     4681 aggattcgta tttcaatttt ataacttaat accaaattta aatgtggagg aaaatataat
     4741 gcttcctttg ttattagatg gtaaaaattt aaaagattat aaaaatcaat tagatgaaat
     4801 cttagatata gttggtttaa ctgatagacg taaacataca ccacgagagt tatctggtgg
     4861 tcagcaacaa cgtgttgcta tagctcgagc tttaattgga aaaccagaga ttttatttgc
     4921 tgatgagcct actggaaatt tagatagtaa aacagggata gaaataatag atttactgaa
     4981 taaaataaac agagacaatg gacaaactat aattatggta actcactcac cagaagcagc
     5041 caaaagtagt agtcgtacta ttacagtttc agatggttta attgtataag gactaattat
     5101 tgtatttgag ataagcattt ttaattatat aaattagaca tatgtaaagt ggatataagt
     5161 taaatactat aaatgtgcta taatattaaa ataacagata aaagtactat attttttatt
     5221 gattatttta attttatttg tttattaaat atctaatgag tattcatact atctctatta
     5281 ttaaaagcag ttaatgatat atagagataa tatggattct tcttacttta ataaatattc
     5341 acgataggac aggatataat atatgttaaa aaaagaaagt attcatttta taaataaaaa
     5401 taggtcactt atagtttgtg aagacaattt tgtttatctc ttgccttgta tgcaattaaa
     5461 aaactggata tcgaatttta ctatatcgtt tccaaatcaa acaataattt ctgataatta
     5521 cacgattatt cctcatggaa gtgtaacttt ggtgtttttt tatgatgcca cagggttaca
     5581 tagtctatta tttggtccaa ctactaaacc aaaaacagta ggaaatattg ctaatagatg
     5641 tgatgttata ctaattatag aatttcaacc tgctggtttt ttcccgctta taggtataca
     5701 gcaaagtgaa cttattgata aagttgttcc attttctata attaatactt cattagattt
     5761 agaaattaaa aaaatattta atgaatcttt atctatagat aaattgattt taaagttgga
     5821 agaattattg atttctaata ttaaaattga atactcatat gagtttattt tggcaattca
     5881 gttgattatt caaaactctg gaaatatatc ttcacaagaa atttctaaaa aagtatttta
     5941 tagttctagg catttaaaca gactctttaa tcagtaccta gggttgagta tgaagtcatt
     6001 ttctcgtttg gtacgtatta ataaatctat aaaacttcta aacaataata aaacttcatt
     6061 gatgtctata tgtaatgaat taggattcta tgatatacca cattttatta aagattttaa
     6121 aattgtctgt ggaataactc ctcaaaaata ccaagctaat atgtctgatt tttacagtga
     6181 aattgcaaaa ttttaagtta taatacttat aacaaagaaa ggatagtgat aagatgaaat
     6241 ttggaggagt attgctaatt gtcaaagaca taaataaatc aaaaaagttt tatgaagatg
     6301 ttatggagca aaaaatcaca ttagatttag gagaacatgt atcatttgaa aatggatttg
     6361 gattacaaag taattataaa gctataattg gatatgattt accaatatat caacaatcaa
     6421 ataactttca gttgtatttt gaggttaatg acatcaaaca atgggaaagt aagataaata
     6481 gaataggaaa tattgaattt ttgcattata taaaagaata cccatggggt caaagaactt
     6541 ttaggtttta tgattttgat aaaaatatta tagaaatttc agaaagtatg gaaagcgtca
     6601 taaagagact tttaaaacaa ggtttagctt tagaagaaat ttcaaaacgc actatgtatc
     6661 cagttgaatt tattacacaa tttcaacaat tataaaatat taaaatatat aattttttct
     6721 ttatattgca aaaaacaaag gcttggggca taatagaacc agaaaaacct gaatctcaac
     6781 agcaaatggc tcatatggat ttcgctgtta atgatttaga agaagcagtt caatatgcaa
     6841 ttcattgtgg agctacaatc gcagaggagc aatttactga tgactggaga gttatgattg
     6901 acccagctgg acaccctttt tgcttatgtc agatgaaatc aattatggaa agtagccatt
     6961 tttcattgtt atagaccaaa atattgttta ttaattacaa aaaagcacaa cagtatatac
     7021 cgttgtgctt ttacttatta tacatgaata cactaaaaaa tataaatagt agagctttat
     7081 caataatgat aatttttaaa aaatagtatt tttttattga ataatttctt agatatttta
     7141 aagtatataa attttgcata tatgaattgg acattatcaa atgtgttat
//
LOCUS       DAEMHW010000183         7095 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEMHW010000183 DAEMHW010000000
VERSION     DAEMHW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.183"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(50..889)
                     /locus_tag="KPK67_003125"
     CDS             complement(50..889)
                     /locus_tag="KPK67_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG2460053.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGISPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(913..1671)
                     /locus_tag="KPK67_003126"
     CDS             complement(913..1671)
                     /locus_tag="KPK67_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2460054.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1718..2191)
                     /locus_tag="KPK67_003127"
     CDS             complement(1718..2191)
                     /locus_tag="KPK67_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2460055.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2220..2636)
                     /locus_tag="KPK67_003128"
     CDS             complement(2220..2636)
                     /locus_tag="KPK67_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2460056.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2683..3735)
                     /locus_tag="KPK67_003129"
     CDS             complement(2683..3735)
                     /locus_tag="KPK67_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG2460057.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4131..4811
                     /locus_tag="KPK67_003130"
     CDS             4131..4811
                     /locus_tag="KPK67_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG2460058.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(4944..5264)
                     /gene="sugE"
                     /locus_tag="KPK67_003131"
     CDS             complement(4944..5264)
                     /gene="sugE"
                     /locus_tag="KPK67_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBG2460059.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5646..6473
                     /locus_tag="KPK67_003132"
     CDS             5646..6473
                     /locus_tag="KPK67_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG2460060.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         2391 a         1288 c          837 g         2579 t
ORIGIN      
        1 aatataatca atagatatag aatcctaact tacaccaata tatatttatt taaaatagtc
       61 ctaaaatacc tgtaatagct cccactacta tcagtataaa cattattttt attggagaaa
      121 tacctttttt taaagcaaag agtactagta aagtaagtac caaaggaatt aatcctggaa
      181 atatcttatc aagtaaatcc gtttgaagac taaatttaaa atcaccacta gtataactta
      241 tcggtgtaga taaattaata aacctaccta caagtccccc catgactaaa gtacctagta
      301 tagaagcccc ttctataacc ttatttacta ttccaccttg aagtatactt tcaacagctt
      361 ttttcccata cttatatcca tttgtccaca ttccaaaaga aattgaagtc attattatat
      421 attgagctat tacaaataat ataggtcctg caattgatgc tcctccctca gttaaaccaa
      481 tacaaacagc taacactata ggagttataa ctccttgagt taaagtatcg ccaattcccg
      541 ctagtggacc catcaagcct gttttgatag cattcatacc atctccactt atttcatctg
      601 ctccattagc tttttcttct tccatagcta ttgttattcc atgtataaca catccacata
      661 taggttctgt gttaaaaaat gctagatgag tctccacttc ttgttttaac tcttccttat
      721 ctggatataa ttttttaagt actggcaaca ttgaatgtga aaaagctgtt gattgcaatc
      781 tttcataatt gtaattagat tgtgcaaaga aaaaccatct taaccatgat tttattacat
      841 ctttcttgct taacgttttc ttagatactt cattttttat atctgacata atattttccc
      901 cctcacatgt atttaagatg tagcactatc ttttccgtac ttaattgaag taattgtaag
      961 agcaagtatt actccaataa cagatattac tattatatct actttaaaat aaacagccat
     1021 tagtattcct aatatgaaaa agggcatagt aaacttgttt ccaatcaatt ttaaattcat
     1081 agcaatccca agtgctggca acatagcccc aattacatta aatacatgta acacattatc
     1141 attcattact tccaacatac catctatcgc atttggacca aaataaacaa ctaataatgc
     1201 tggtataaaa ctcattgcaa ataacagtat ctgtggtggt actatattta taaatgctac
     1261 tctatcaaca ttcccttttt gaacctctct atcagcccaa tgtgcaaaaa aagaatttac
     1321 tgtcatcttt ccaatccaaa gtacagttgc aacagttccc aatggtacag ctattgccat
     1381 tgcagtactt acatctagct tagatgctat agctaaagca gtacctacat atcctgcaaa
     1441 agctgggtcc cccatttgag ctccccctgc tgatataaaa cctagatata tcatatttat
     1501 acctgctcct ataattgctc cttgaacagg gtctcctaat ataattcctg ttacaaatcc
     1561 agccaataga ggtctattcc atgtaaaaaa tccaacataa gtcaaccacg gactccaaga
     1621 aagataataa aaaattgcta ttaatactgc ttgaattagt gaaatgtgca tattcttcct
     1681 ccctattttc ttttaaagat ttacaagata gtcttcttta caataatgtt cctacatcaa
     1741 ttttctttgc atctggtact atttgtataa agcatttaac atcttttgat attagctctc
     1801 taaatatttc tttttcttca tctgacacag atatattttt ataaaaactt tttctaccaa
     1861 gccctgctcc catacctcca acattcaatt cctttaaatc taccccgcta tttactaaac
     1921 ctaagacagt tttagggtat ttgaccaata ctattaaatt ttcatctcca ttatgtgatt
     1981 ttaagacttc agcacctgat tctacatcat gtacttcaac cttaacagtt gatggagctg
     2041 ccatttgtaa aacttgaact ataaatgggt cctttactac taaatcatcc acgattacta
     2101 tcctttttgc cgatgtaatc ttacaccaag cagtaataac ttgcccatgt attaatctat
     2161 catcaattct tgttaatgct atattactca taattattcc tccagatttt taatttattt
     2221 tacaacttta aaacagtttt aacatctttg attcctgata tcccaacttc ctcacataaa
     2281 tttttcaagt cttttaactt catagtatct ctgtttaaaa ataactctaa aagcataggt
     2341 aaatttacac ctgtcaaaca ttctaactca cataccttag caaatttatt tattataata
     2401 gcagatgtat taaatggagt tccgccataa aaatcaagca atactaaaac cccttcatcc
     2461 ttatatttct caattccatc ttctaacttt ctactaaact catctatgct gtcaccatgg
     2521 tttaatccta aagtttctac attttcctgt ttgcctacta ttaattcagc actttctaac
     2581 attgctttag ccatatttcc atgtgtggct ataattattc ctgccatatg aatcatacct
     2641 ttcttaacct tttttatatg gattattatt tagtactcta atctaattat ctatattaat
     2701 cattatctta ccaaagaaaa tatccctttt gtatactttt tcaaaataag ctggtacttc
     2761 ttctaaattt attctatgtg ttactaattt ttcaacattc atatcaccct tttgcatatg
     2821 atacaatcca gcactccatt cttttcctgg gaatggaaag gagtttccaa accaagttcc
     2881 ctttacagtt aattcacttc ttactatctt ttcaaattgc tctctagtca aagctacatc
     2941 cccatatggt acacctgcat acaatactgt acctcccttt ttagctagca acagtacttg
     3001 accacatgtc agtggagtac cagccgattc aataacaata tctgcaccat ctccatcagt
     3061 taaccttttt atttcttcta caatattctt ttcttttgca tttatacaaa tatctgcccc
     3121 taactctttt gccaagtcta acttctcatc aaatacatcc acagctatta tttttgtaga
     3181 accaaatatt tttgcccatt gtattgaaaa taatcctatt ggacctgtac caagtactac
     3241 aacagtatct ccaactttag cttctgacct aaaaagacca tgtcctgcta tacatactgg
     3301 ttctagcgca gcggcagtct cataacttat ctcatctggt atttttatta aatttctctc
     3361 tttcaccttt gtatactctg caaaacaacc tccaagttct ttatttccta ttattgctac
     3421 attattacat cttgaataca agcctttttt acattcatca cattcaaaac atggcatagc
     3481 tggacaaaca gcaactctat ctcctatttt aaaactcctt acttctttac caacctgtgc
     3541 cacttctcca gaaaattcat gccctaaaat ttctccaacc atatgtggac ctgtctttga
     3601 atattttgat atatcagaac cacatattcc agctactttt actttaatta ttacatcatc
     3661 tttttctaat atttttggta catccacatc ctccactctt gtgtctctta taccatagaa
     3721 tctaactgac ttcactatta accaccccta ctttttattt gagaatatat ccctattgtt
     3781 tatatgttaa atatctaaag caactttcat gccaatatat gtaagcattg tattcatgca
     3841 ttttataaaa ttatcattaa atgtgtgtca aagtgtgtca aaagtttctt atattaaaac
     3901 ttatgtgtgt caatataaac gacactaaca ttttatatta gttctcttat tataagtttt
     3961 ataaaatgta ctttttttgt tatttttata aaattgttcg gaaatttccc ttctatttgt
     4021 gatataatat taaatataag gtatcaatat aaattttcat gcgaatatca gaactttttt
     4081 gacactaatg ccttattcaa tataataatt taactttttg gaggttgttt atgaaaaaag
     4141 tgtatcaagg aaagactaaa gatgtttttg aattagataa tggaaattac ttacttaaat
     4201 ttaaagacga tgtaactggt aaagatggag tatttgaccc aggcgaaaat agtgtaggtc
     4261 taagtataga tggaattggt agagctaatt tagagacatc tgttaagttc tttgaaattc
     4321 taaacaatgc tggaattaaa actcattatg taagtgctaa tttagaagaa gcaactatgg
     4381 aagttctacc agcaaaagtt tttggtaatg gattagaagt tatttgtcgt tatagagctg
     4441 ttggtagttt cttacgtcgt tatggttcat gtgttgaaga aggtgctaaa ctagattgtt
     4501 acgtagaagc aactttaaaa gatgatgacc gttgtgaccc tcttataaca tctgaaggtt
     4561 tagaagcttt aaacattatg actcaagctc aatttgattc aatgaaaagc atgactcaaa
     4621 aaatttctaa catagttcgt gatactatag ctgaaaaagg tcttgagctt tatgatatta
     4681 aatttgaatt tggattctac aatgatgaag taatcttaat agatgaaata gcatctggaa
     4741 atatgcgtgt ttataaagat ggtgtaatag ttgaccctat ggatttaact gctcttttat
     4801 tagataaata gtatatacat ataatttaca aatataataa acttataaat aagataagct
     4861 atgaagtaaa gactttttaa atgttgatta tttttaaaca ttttctcgcc tttaatttca
     4921 tagcttattt aaattaatat actctaatta gtagataata gctttaaccc tacaattccc
     4981 accacaataa aacctataca tgcaattctt gatatactaa taggctcatt taaaatagcc
     5041 atgcctacaa tagctgtacc gacggtacca attccagtcc atattgcata tgaagttcca
     5101 agtggaagat gttttagtgc taatgacaaa aaataaaaac ttacaatcat tccaacaaca
     5161 gtaagtatac ttggagttaa tttagaaaat ccatgtgaat tttttagttg aaaagcccaa
     5221 aacacttcaa acaaacctgc aacaaacaaa aataaccatt tcataaattc atccctcttt
     5281 tcaaatatgt ctcttatttt tccaataaaa taacctgaga aatattataa aatatctccc
     5341 aggtttttgt ccttccgtgt tcacaacaaa aactatgttg tgggttttat ctcggaccag
     5401 accaatcatt aataattgcg gaaccctata aaactctatt aattattcta ttttatctat
     5461 aataatacta tcaacatata taaaaaaagt caatatatgg gttccatcta taataaaaat
     5521 aacttttata taccttttaa tattcttatt cacttctctc catacccatt taggtcaatc
     5581 taagaatatc taatttttat ttaatatgtt ataataacat aaaaatattg aaaggagtaa
     5641 tttctatgaa tttctataaa attgcagtat gtcagatgat tactacagaa aataaaattg
     5701 aaaatatcaa tcatgctgta gatatggtaa cagaagctgc catcaatgga gctaaaattg
     5761 tagttttgcc agaaatgttt aactgcccat atgaaaataa atattttcct aaatttgctg
     5821 aagaatatcc aggtgaaaca acaacaatat tgagtaaatt agctgagaaa catggaattt
     5881 atctagtttc tggtagtatt cctgagttag aagatggaaa aatctataat acttgctatg
     5941 tatttgataa aaatggagct ctaattggaa aacatcgtaa aatgcactta tttgacattg
     6001 aagttacagg aaaagttagt tttaaagaat cagatactct tacagctgga aatgatgtca
     6061 ctgtaataga taccgaatac ggaaaagttg gtatagctat ttgctatgat attcgtttcc
     6121 ctgaattatc acgtttaatg gctcttaaag gtgctgaaat cgttatctta cctgctgcat
     6181 ttaatatgac tactggtccc gcccattggg agttatctat aagaatgcgt gctttagata
     6241 atcagatatt ctatgttggt gctgcacctg ctagaaatat gaatgcttct tatatagctt
     6301 ttggaaattc aagaatttca gacccttggg gaagaataat tgctcaagct gatgaaaaag
     6361 aatgtattat ttatgctgac attgatagag atttaattcc tgacataaga caacagttac
     6421 cacttcttaa gcacagaaga actgatttat atgaacttaa tactttgaaa taaaacagaa
     6481 cataacccca taaatcataa attaagtgtt aaaaaaattt atggggttct cattaatttg
     6541 atttaaaatt tatttcataa aaataatgct aaaattaatt taattaaata atactaataa
     6601 ttactaatga ttatgctaat tatcagcatt tactaattta atcactatta gttatttctt
     6661 ttattaaata tctttacttt tttacttatt atgtcttcta gctttacatt tggattatgg
     6721 tatttctttc tatttttagt cacatctttc tattgaatta cttgcttaaa acaccaatgc
     6781 acacatgcca gataatgttc acaattatgt agaaatcttg ctctaccttc ttcaatgttg
     6841 atattatctc tcctagttga tattatcttt cctaaaattt cttttaatta aaaaacaaca
     6901 ttagttgata tatcaagtat attttaagca actgaaaaaa acaataataa ttgatatatc
     6961 aactaattaa tgtagattaa ttcattttta taatttatat actttttaat taatctacct
     7021 tatttaatta aaatccttcc tttctattta tccattttca acttaaatta taagatttca
     7081 tcagctatat ttttg
//
LOCUS       DAEMHW010000184         7054 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEMHW010000184 DAEMHW010000000
VERSION     DAEMHW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.184"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..301
                     /locus_tag="KPK67_003133"
     CDS             <1..301
                     /locus_tag="KPK67_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG2460061.1"
                     /translation="YIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFEKLEV
                     YFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQLT"
     regulatory      394..486
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            586..1380
                     /gene="thiD"
                     /locus_tag="KPK67_003134"
     CDS             586..1380
                     /gene="thiD"
                     /locus_tag="KPK67_003134"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBG2460062.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1404..2192
                     /gene="thiM"
                     /locus_tag="KPK67_003135"
     CDS             1404..2192
                     /gene="thiM"
                     /locus_tag="KPK67_003135"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBG2460063.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2215..2847
                     /gene="thiE"
                     /locus_tag="KPK67_003136"
     CDS             2215..2847
                     /gene="thiE"
                     /locus_tag="KPK67_003136"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG2460064.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDDADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            2986..3288
                     /locus_tag="KPK67_003137"
     CDS             2986..3288
                     /locus_tag="KPK67_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2460065.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3354..4103)
                     /locus_tag="KPK67_003138"
     CDS             complement(3354..4103)
                     /locus_tag="KPK67_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460066.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4097..5059)
                     /locus_tag="KPK67_003139"
     CDS             complement(4097..5059)
                     /locus_tag="KPK67_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460067.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5063..5521)
                     /locus_tag="KPK67_003140"
     CDS             complement(5063..5521)
                     /locus_tag="KPK67_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG2460068.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5877..6233
                     /locus_tag="KPK67_003141"
     CDS             5877..6233
                     /locus_tag="KPK67_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2460069.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6305..7021
                     /locus_tag="KPK67_003142"
     CDS             6305..7021
                     /locus_tag="KPK67_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460070.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQFHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
BASE COUNT         2772 a          835 c         1181 g         2266 t
ORIGIN      
        1 atatattata agatgtaaag atgattctct atacactgga tataccagta atatagtcag
       61 aagaatgaat gaacataagt taggaattaa ttcaaaatac acaagagcaa agggatttga
      121 aaaactagaa gtttattttg tgaccaacac aaaaagcaat gctatgaagt tagaatacta
      181 tataaaaaaa ttaactagaa ataaaaaatt gtctataata aaaaacccaa gtatacttat
      241 aaatttaatt gataataaag aagattatat tattggtaag gaaatagaac aattaacata
      301 aatatttata gtaacattat aatgggtgca tatagtgtaa aaacttgaca ataaatacta
      361 ttgtaatata atgaagttat aaatatagta aattaaaata gctaggggag ccaagagctg
      421 agaggtatac cgacccttac acctgatctg gataatacca gcgtaggaaa gcttagtaaa
      481 aattcaagta tgtattattt gtgatatatg ctatttctaa gctataccta ttgtaggtat
      541 agctttttta attaagttat ttaaattaaa ctttaggagg caacaatgag taattataaa
      601 ataccaacac ttacaatagc tggatctgat tcatctggag gagctgggat tcaggctgat
      661 ttgaaaacgt ttagtgcaat tggtacttat ggtatgagtg taataacagc tataacagca
      721 cagaacacta aaggagtttt tgctgtagag gatttaaata agaaaataat aaaaaaacaa
      781 atagaggcag tctttgaaga tatacctcca agagctgtaa aaataggaat ggtttcaagt
      841 cctgagatta tacttgaaat agttgagaat ctaaagaaat acaatccaaa atatttggtt
      901 gtggacccag taatgatatc taaaagtgga tattatctat taaagccaga ggcaaaagaa
      961 aacttaatta agtatttgat accacttgca tacataatta caccaaatat accagaggca
     1021 gaagaaataa caggaataaa aatacataat gtagatgata tgaagagagt aggagaagaa
     1081 atactacaac ttggacctaa atttgtactt atgaaaggag gtcatttaga tggagaagca
     1141 gtggatattt tagttggtaa aaatatattt aaagtttata aaagtgaaag aattgataaa
     1201 aaaaatacac atggaacagg gtgcacatta tcttctgcaa taacatctta tctagcatta
     1261 ggatatgaaa taacagaagc agtaaatcta agtaaaatct atataacaga agcaataaaa
     1321 agaagttttg atataggtca tggagtagga ccagtacatc atttttataa atttgagtaa
     1381 taaaaaaata ttggaggtag aatatgtata atttaattaa agatgtaaaa aaattaaatc
     1441 cattggtaat tcattatact aataatgtaa ctataaatga ttgtgctaat gtaactttag
     1501 ctgtaggagc aagtcctctt atgagttttt catatgagga agtagaagaa atggtaagtg
     1561 tagcaaactc tgtagtaata aatataggaa ctatgaactc aaatatgttg gatttattcc
     1621 tattagctgg aaaagcagca aataagtata ataaacctgt tgttttggac ccagttggag
     1681 tatttgcaag caaagcgaga gcagaactta caagtagact tttaaatgaa gtcaaattta
     1741 gtgttgttaa agggaatgta tcagaaataa agtttatagg tggctttaat gtaaggggta
     1801 aaggtgtaga ttcatttgat gaagaagaag attctactga gataataaga aagattgctg
     1861 aaaagcttga atgtgtagtt gtagcaactg gaaaaataga tataataact aatggtaaag
     1921 gtacatataa aataaacaat ggaactgata aattaaaagg aattacagga actggatgta
     1981 tgactgctag cttgattgca agttttatgg ctgttactga gaatatatta gaagcagcaa
     2041 caatgggtgt tcttacaatg tctttaagtg gggaattagc taatttaaat aatccaccaa
     2101 taggtacatt taaagaaaat cttatgaatg ctatttatca aatggatata gatactttaa
     2161 gtaaaaattc taatattgaa tttttaaatt aataaagagt aagggagact taaaatgatt
     2221 gataaagaat ctttaaaaaa atgtttaaag ttgtatttag taactgactc tgaaatgctt
     2281 aaaggtagag acttttataa atgtcttgaa gatgcaatat cttctggaat aacaactgta
     2341 caacttagag aaaaaaatgc ttctggaaga gagtttttaa gaaaagctat gaaacttaga
     2401 gaaataacta aaagatatgg tgttaagttt ataataaatg atagagtgga tatagcactt
     2461 atttgtgatg cagatggagt acatgtagga cagagtgaca ttgatgttag agaggttaga
     2521 aagcttatag gaaacaataa aattctagga gtatcagcaa gaactttaga agaagctatt
     2581 tgtgcaaaaa atgatgacgc agactattta ggtgtagggt ctatattcac aacatctact
     2641 aaattagatg caaaatcagc ttcgtttgaa actgttaaag aaattaaaga aaaagtagac
     2701 atgccatttg tactaattgg aggtataaat ttagacaata tagataaact taaatgttta
     2761 gaaagtgacg ggtatgctat aatatcagct atattaaaag ctgaagatat ctctaaagaa
     2821 gtggaaaagt ggacattaaa aatataaaat tggctctggt agtgaaaaaa atggaaaaat
     2881 ttgatataat ctaagagtaa aatttaagca attataaatg ataaactttt ctacatatac
     2941 tttttaatca agatatatgt agatttggtt aaaaaggagc tataaatgat taggatattg
     3001 ttagtgtgtg ttggaggcat gtcttctacc ctattggtaa ataaaatgga gaaggatgca
     3061 aaaaaaagaa atatagactg taaaatatgg gcagttggag aaggagatat aaaatcggag
     3121 cttgataact ttgatatttt acttcttgga ccacaactta gatttatgct tgatgacgta
     3181 aagtctatag ttggagatag agctcctgta tctataatag atatggtaaa ctatggtact
     3241 tgtaatggtc atgctgtgtt aaattcggtt ttagaaatat taaaataaat aataaaagtg
     3301 gattctatat aatagagtcc acttttatta tttgttatta ataggcttaa aacttagctt
     3361 accatttctt tttttaatct ccacatagca agtgaaaaac ttgaacagca caatattaaa
     3421 aatagtagga taacaggcag tatatctcca ataagaatag tctcaccaaa tattaagcta
     3481 cgtaaagcgt taataacatg ggtaaatgga ttgagcatca ctgctacttt taatcctcca
     3541 gataaacttt ctatagggaa aagagctgaa cttaaaaaga aaataggaag tacaattgca
     3601 gtcataattg tttcataaac tatttcattt ggcaataata aacttataga ataagctaga
     3661 cttgataaaa agaaggctgt cataaatatc agtactatca taagtaggat tccaccaatg
     3721 ccagattcta tccttactga gaagaatata cttactatac acaaaatagt tacttctatg
     3781 aaagaaacta agatagcttc taataattga ccaaggacga ttgaatacct actgataggt
     3841 gcaattaaga ctctatagaa acttccactg ttcttcatta taaagtttat aataccacca
     3901 ctactacaag aagaaaacac aaccagtaca atgatacctg gtagtataaa agcagtataa
     3961 ttattgatat ttatattatt catagtttgg tttgcaatag aactataaag cacaagccaa
     4021 agaaaaggtt gtaatatagt aactacaaat gagattggat ttttaaaacg ccacttcata
     4081 ctacgccata agatatttaa cattcccatt cctcctttaa gcttttttta gaacttgtaa
     4141 gtgctaaaaa tacatcttcg agacttggct ctacaatttc tattgcatca aattctatat
     4201 catgttctaa aagccattta tttattaaag taaaagcagt tctactatcg tttacactta
     4261 taaaaattga attttctcgt acgctcataa actttactaa accagtactt ttaattgaat
     4321 ctttatattt ttttatatct tcagtattat ggaaagttat acgtagtata ttttgtctaa
     4381 tgtaactacg taaacttgat ggtgttcctt gtgctaaatc ctttccattc ttcataatac
     4441 aaatattatc actcaattgt tcagcttctt caagataatg agtagttaga aatatagttg
     4501 tgccatactc atctctaatt tttaataaca tatcccacat agattttctt gaatcgacat
     4561 ccataccaac agttggttca tctaaaaata ggattttagg agatgatacc atattcatag
     4621 ctatgtccaa tctacgtttt acaccccctg aatatgatga agtaggatat tttaagtaac
     4681 tagataagtc aaacttatca attaaagagt ctattctttg ttttgcaatc tgtggttcta
     4741 ctttatacat tttactttga aataccatat tttccataag ggataagtgt tcatcaatag
     4801 agatttgctg tgctacacaa gcaatttgag tacgtatcca agaaggatta tctactaaat
     4861 cttttccaaa cattgttaca tttccagatg taggtttgta aaaggtagtc aaaatattta
     4921 taagagaaga tttaccagca ccattaggac caagtaaaga aaaaatttct ccagcattta
     4981 cattaaagct tagaccattt aaagctttca caccattttt atattctttt actaaattgt
     5041 ctatttcaat cgctaacata tattattcct cctctttaag tggaaatatt atctctgtaa
     5101 tatagttatc tgggtttcct tttatcaaca tacctggtcc tttaataaat acttctctcc
     5161 aaggagtttg tatttttata ttattttcat gaatatattt aaatatcatt tcatacgttt
     5221 ttggcaaatt actataagag cctgtgtgag taaaaaataa agcttttgtt ttaggaaatt
     5281 cttttgtagt tatacccata cgatttggag tttcagcagt tggaacacat aattccattt
     5341 ctgcaacacc agttttttgg tcaacatcat agtagcaaaa gaatggtgca ccgttagact
     5401 ttccttttat agctttaaat acatttggaa aatactttgt tgcctcagtc ataggtccat
     5461 tataacgcat ggtagccact gtgactgatt cgatattttt gatttcaatt ttgcaattca
     5521 tttgtttacc tcctaatgtg tgttcatatt cttactagat ttttaagcat taatacatat
     5581 aagattttga ttagttgaaa acatataatc ttatctcgtt tgcttgtttt caagtctaac
     5641 ataacataaa atatatatcc tctcatttga tgctccattt tgtaaagtta ttatttaagc
     5701 tttactttag attttttagc aaattaaaat aaagcaattt ataaaataag tatttacaaa
     5761 ttaagtgtat taatcgtata atacacttaa taggatacta gataaagtct gaaataaagg
     5821 cgtatttttt taaagtaagt gtactatcgt attaatacac tagaatagga ggaatcatga
     5881 attttgaatt agataataca actcccattt atttgcaaat agtaaaatat ataaagagac
     5941 aaatagtaac aggagagttg aaaccaggag agacaatacc atcaagaaga gaaatggcac
     6001 ttaatctaaa agtaaactta aatacagttc aaagagcata taaggagatg ggtgatatga
     6061 atataataaa tacttttaaa aattatcaaa gcagtgtaac agttgatgaa aatatattaa
     6121 aaaatttaaa attagagctt ataaatgaat cactatctgt ttttatagaa gatatgaaag
     6181 caataaatgt tagcaaagaa gaagttttaa aaataataga agataagtat tagtttttta
     6241 acaaatagaa aacttagttt agaaagttta tttataagca tattggatgt ggaggatagt
     6301 aaatatgata gaaataaaga atgtcagcaa aacatataaa aggatgcaag ggcttaaaat
     6361 aagaaagata gaagctttaa aaaatgtaag ttttaatata gaaaagggaa aaataactgc
     6421 attacttggt ataaatggtg ttggtaaatc aactatgtta aaagcaatag caggacttat
     6481 aaacattgat agtggagaaa ttcgtataga tggagaaaaa ataaatgaaa aggtatacaa
     6541 taaattggct tttgttccag atgtacaatt tcactttagc aatacaacaa taaaagaaac
     6601 atttgaattt atggaaattt tttattcaaa gtggaataaa gaaaaatcaa aagaaatgat
     6661 ggacatattt aaattggatg aagatgaaat aattgataac ttgtcaaaag gaaacattgc
     6721 aagagtaaaa ttaatacttg gattttgcca agacccagag tacatacttc ttgatgaacc
     6781 atttacagga attgatttgt ttaagaggga agagtttata ggagtaatag cgcaatatat
     6841 ggaggaaaat caagccatta tcataactac tcatgagata gtagagatag aaagtttagt
     6901 tgatgaggtt gtaattcttg atgaagggca gattataact tcatttaatg cagaagaatt
     6961 aagagaaaga gaaggtaaat ctatattaga taagatgagg gaggtatata agaatgaata
     7021 aacttctata tctaataaat gtagatttga gaag
//
LOCUS       DAEMHW010000185         7026 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEMHW010000185 DAEMHW010000000
VERSION     DAEMHW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.185"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            437..1087
                     /locus_tag="KPK67_003143"
     CDS             437..1087
                     /locus_tag="KPK67_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2460071.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            1403..3469
                     /locus_tag="KPK67_003144"
     CDS             1403..3469
                     /locus_tag="KPK67_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG2460072.1"
                     /translation="MKEVVGSLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYIWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKEINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYIYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEIKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGTRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            3486..5441
                     /locus_tag="KPK67_003145"
     CDS             3486..5441
                     /locus_tag="KPK67_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG2460073.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLERSITQNRDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGIYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            5457..6643
                     /locus_tag="KPK67_003146"
                     /pseudo
     CDS             5457..6643
                     /locus_tag="KPK67_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
BASE COUNT         2646 a          821 c         1331 g         2228 t
ORIGIN      
        1 ctatagtaca ctagcatgat ttgaagtaca acgatttaat ctatagtaca ataacttgat
       61 ttgcaataca atgaattaat ctatggtata atagtttaat ttgaagtaca atgaattaat
      121 ctatagtata atagtttaat ttgaagtaca acgatttaat ctatagtata atagtttaat
      181 ttgaagtaca atggtttaat ctatagtata atagtttaat ttgaagtata acgatttaat
      241 ctatagtaca ataacttgat ttgcaataca atgaattaat ctatagtata agagcatgat
      301 ttacagtaca ataatttaaa ttgtagtaca aaagtactat caaataagaa tttctacttt
      361 aaaaatatta ctaaaagtaa ttaaaatcca ataattcaat ataatatggc aaaagaatag
      421 aaataaggtg agagtagtga aagtaaaatc aaatttagat gaaattttat atcataagat
      481 tattgaaagt cttattcgtg gtgaatactc tgtaggtcaa aaaatattac taaatgacct
      541 ttgtgaaaaa tttgaagtaa gcagaacacc agttgtacaa gcagtaaaaa tgctaaataa
      601 agatggagtg cttactatta tgacaaatgg taaagtgtac gtaccagagt atgaatatga
      661 tatggtaaaa caagtttgtg aaacgagaac actgattgaa acatatgctt tagaaaaaat
      721 gatgcaagaa gaggaagagg tttttcaaca aaagctagat gtaataaaaa gatactctga
      781 taaatgcgag gagtactatc gtcaagaaaa atctgtagaa cttgcactag cagatttgga
      841 attacataaa gccattgtag aaggggcaaa taatgagatt ttaaatgatg tatatgttgg
      901 tatacaagga agatttatag tagtgaacta tttaattcga ccattaaaaa atcgtaatta
      961 tgaaggaaca gtacaagacc attttgaaat attaaaattc attgaagaca gagatacaaa
     1021 aaaagcagta gataaactaa gaaatcatat tcaaggaact attaaaagat tctgtgaaga
     1081 tgaataataa gttttgaagt tactatatat acttatctat taagttgcta aatgtagtct
     1141 aagtataatc taattatagt ttaaacataa tctaaatata atctaataaa tataatctaa
     1201 atatagtgta aataagtcta agtatgaatt aaatatagat taagtatgga ctaagtatag
     1261 attaagtatg aattaagcat ggattaaata tactttaaat tacaaatatt acaaggaagc
     1321 cagaaggctt cctaaaaaaa taacctttat aaaatttttt attttaaatt taaaattttt
     1381 tattttaaat ttaaaataaa aaatgaaaga ggtggtaggg agtttgaaaa aaattactaa
     1441 agaaaggata gaaaatacta tgaaaaagct atttacgagt gaaacatttt tggaatatca
     1501 atttataagt gattgccaaa tatcaccaga tggaggatat acagcattta ttgtaaaaaa
     1561 agctgatatc aaagagaatg gttacacaag ccaagtttat atattaaaca ataaaactgg
     1621 cgaattaaaa caaataacgt ctataaactc tgttggagca tatatctggg aagatgaaaa
     1681 tactatcttg ttccctgctt tgcgaaatga aaaagtaaaa gaagctgtca aaaacggtaa
     1741 gcaatgcatg agttactatg ctttatcttt aaatggtgga gaagcagaag aactttttag
     1801 actccctatc aaaggtggta aattgaatcc tataggaaaa ggtttgtatg cagttattga
     1861 ttcttatgac aatgaccgac ctattgtaga gggattgcca gaagaagaac aacagaaaga
     1921 aataaatgct tataacaaaa gacattatga acatttcaaa gaaattcctt atgctgtcaa
     1981 tggggaggga tatattagca gaaaaagaaa acgcctatat atatacgaca gtaatataaa
     2041 tgagttgaag gcaattacag cacctatgtt taatgttgta ggtatgaaaa taagtgatgg
     2101 aaaaatctta tatatagggc aagagtttaa agatgtaaaa ggtttgaaaa atggggtcta
     2161 tgtatttgat acaaacacaa acacaaatgt ctgtatatta gaaaaagata agtatattat
     2221 aaaaggtttt gaattatatc aaaatcaagt gatactaaac ctaactgatg ctttatctta
     2281 tggaaatgga gaaaatggag atttttatac tattgatatt gatacgaaag aaataaaact
     2341 tctttcaggg catcaacatc attgtattgg aaatacagta acatctgatg ttaaaatggg
     2401 tgctggtcag acaacaaagg tagatggaga atatatttat tacacttcta cagtagatat
     2461 ggactgcatt attgaaagaa tacatattcc aacaggaaaa caggaaaaag taacacagac
     2521 aggttcagtt gattttatag atgtaaaaga tggcaatatt gtatgtgtgg gttgtttagg
     2581 aaatggatta ccagaagttt atacagtaga gaatggaaca ttgtgtaaga aaacacattt
     2641 aaatgaccat attttagagg aatataaaat ttctgtgcca gaatacatag agtcaaaagg
     2701 aagttctaaa tgggaaatac aaggatacgt atataaacca gtagattatg aggtggggaa
     2761 aaaatatcca gctgtattgg cgattcatgg aggaacaaga ttgacatatg gaccatattt
     2821 tatgcatgag atacaggtat ttacaagtgc aggttacttt gtattcttct gtaacccaag
     2881 ggggtctgaa ggtcgtggca atgcttttgc agatattagg aaacagtttg gagatattga
     2941 ttatatagat tttatggaat ttacagatac tgtacttgaa aaatatacag acattgataa
     3001 aacaaaattg gctgttgaag gtggaagtta tggtggattt atgacgaatt ggataattgg
     3061 tcatacaaac cgttttgctg ttgcttgtgc tcagcgttct attgcaaact ggagtggcat
     3121 ggagggtaca acagacattg gttattattt ctgcaaagga caaacaggtg cttctcatat
     3181 ggaaaaccac gagcttcaat ggaaacagtc acctcttgct tatgctgata aatgtgttac
     3241 accgacttta tttttacatg gtgaaaaaga ctatagatgc tatatgcaag aagcattcca
     3301 gatgttttca gcattgaaaa ttcatgggtg tcctacaaaa ctttgtctat ttgaaggaga
     3361 aaaccatgaa ctgtctcgtt ctggtagacc aaaacaaaag ctacaaagat tagttgaaat
     3421 gctagattgg ttttcggtat atataaaaaa agataacata ggacagtaga aaggagaata
     3481 taaaaatgga aaaaataaag attgaaagtt cagatatttt tgaatatata ttccctcatg
     3541 atatttcttg ttcacctgat ggaaagcata ttgcttatat tatatcaaat atcaacgagg
     3601 aaaaagactg ctatgaacat gacttatatg tgatggacat caaaacagaa aaacaaatac
     3661 atatgactca aacaaaagat gtaacatcat ttagttggat ttctaacaca gaactacttt
     3721 ttacatctaa aagaaataag ccaaaagcag gtacaacaga tttttacact atttctatag
     3781 aaggaggaga agcaaaaaaa gcattttcta ttccaaaagc ttgtagtgta cctgtatcac
     3841 ttggaaataa attgtggtta ctgacaacaa aaaatccaac ggattctaaa aaatcagaac
     3901 cagatagagc agtagagggt gtagattatt ggacatttac agataaacct tttataagag
     3961 atggagaaaa tttttctcaa agaagacgtg taacattaga attatatcaa gaaggtgaaa
     4021 atataacaaa agccattaca ccaaaatttt gtgaagtagc aggtatagac gtttcttctg
     4081 ataaaaacag aattttatat actggtcaga tttatgaaga ttgtgcaact cctttttctg
     4141 gactttggga gtatcatata gacagtggtg aaaccaaaga acttgtacca caaggaaagt
     4201 accaaataag tttggcaaaa tatattggca aagataaagt aatgttgcaa gcttcaacat
     4261 tagaacgttc tattacacag aatcgtgata tttttatact tgatttgagt actagtgaaa
     4321 taaatatgat tgctagtcca gatggtatgt atgctacatt gctggatgta gatgcagtat
     4381 atggtggtgg acgtagtaat aaagttatag gagataaatt tattggtgca agaatttgca
     4441 gaactatgac agaatttaat gaatttgata caaagacagg taatatacgt atcatcacaa
     4501 aagtagatgc ttttacatct tttgatattt acgacaacac catgtacaca gtaatgttga
     4561 aagattatga gttagcagaa atatatagca ttgatatgac tacaggtaca atgaaaaaaa
     4621 tgactgcttt ttctaaacca tatttagata ctcataaggt ttcattacct gaaaaattga
     4681 cgtttgtagc aaaaaataaa gaagaagttg atggttttgt tataccacct atagatgcta
     4741 aagaaggcga aaaatatcct gctgtattgt tcattcatgg tggacctaaa tgggcatatg
     4801 gctatatgtt tacacacttg aagcagtgtg taacatcaaa gggaatatat gtgatttatt
     4861 gtaaccctca tggtggtgac ggttatggtg aaaaattctt agaaatggta gaaagatggg
     4921 ggtatgttga ctatgagcat cttatggaat ttgtagatac ttgtattgaa aaatatcctg
     4981 ggattgatgc agaccgctta ggggttgcag ggggaagtta cggtggttat atgacaaact
     5041 ggataattgg tcatacagac cgtttcaaag cagcagtttc tcaaagaggt attagtaact
     5101 tgattacagc ttctttaatt attgactttg gagatagaat tatgaagcag acttgtggag
     5161 ataagacgcc ttggaatcat gaagaggttc tttggaatca ttcacctatt aaatatgtga
     5221 aaaatgttaa gacaccaaca ttgttcttac atagtgatag ggattataga tgttttatgg
     5281 gggatacttt ccaaatgttt acagcattaa aacagcttgg tgtagataca gaaatgtatt
     5341 tgttccacgg agatacacat ggattgtcta gaaatggaag accatctaat agaattgcac
     5401 gagcaaatgc tattgtagat tggtttgaaa gatatttata ataagggggt tattctatga
     5461 gtgatatgtt attatggttt agtgtcgtat tagttttatt tgctattggg gatttaattg
     5521 caagcaaaac aaaagcaaaa gtatcagctg tatttgttac actactttta tttttgattc
     5581 tatttgtcac aaaagcaata ccagctgata ttattgaaaa agcaggtatg accgcagcag
     5641 ctagttggag tgttccaatg attatgttta gtatgggaac aatgttgaat gttaaacaat
     5701 ttatagacga atggagaaca gtattgacag catggcttgg aattgtagca gttatagtct
     5761 gtgtctcatt atgtattcct ttgtttggaa aatcaacagt attgacaagc ataccagtta
     5821 taaatggagc tttacctgca ctattatgac acaagcagca ctagaaaaag gactgacact
     5881 tgcagcagct acagcaacag ttgtatttgc aattcagaag tttattggta caccaatagc
     5941 ttcaagagca gcgttacaag aggcaaaccg tcttttagta gaatatcatg aagcaaaatc
     6001 aaaaggcatt gatttagcaa atgttgatac tgataagaaa gaagcagaga atacaggcac
     6061 aaaagtaaaa caagctttct gtgaaaaata cgataaatat tattctacaa atgtttgtat
     6121 attttttata gcactattct cttatctagg gtatgaactt tcagagatta ttcatgtaaa
     6181 ttattctatt gtatgtcttg ttgttggtgt tattgtaaca cgtattggta ttgtaccaaa
     6241 agacattttg gaaaaaggaa aaatcaaggg atttatcaac atggttgtgt ttgcagctgt
     6301 tattccttct ttagcaaaag tttctttgac agatttaatt agtctttttg taccaattgt
     6361 gggtatgttt gcagcttcta tcattggtat attccttatg atgaaggtgt taccaggttg
     6421 gaaaattatc ggttctaaac ctcttgcttt tggagtaggg ttttgtcaga tgttgggatt
     6481 cccaactaca tatttaattt caaatgaagt gtgtaatgct gttggagaaa cggaagaaga
     6541 aagagcatat ttgatgtcta agattatgcc taagctggta gttggtggta tggcttgtat
     6601 gatttctatt gtggtagctg gtcttatggt accaatgttg tagataggtt tcaaagttaa
     6661 aaaagtggta ttaatttata ggagaagcta aaaagtagta ttaatttata aataaagcta
     6721 gaaagtcagt attaatttat aaacgaattt tatattagtt aatttattat aagaatattt
     6781 tctttttagc attataatct tgattttatc aagaaacaga ttttttattt gctattctaa
     6841 tagttacagt ttatttttat tttctaaaac aaaactttaa cttgatttat ctgtaaatag
     6901 ggtatttaat aatatttttg gaaaatcttt agaaagaatt aaaaagagga gaaaaaatgg
     6961 agtgcctcaa agtgaacttt tttagttcat aaggcactct tttttgtatt aaatcgaata
     7021 tatttt
//
LOCUS       DAEMHW010000186         7007 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEMHW010000186 DAEMHW010000000
VERSION     DAEMHW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.186"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            189..2234
                     /locus_tag="KPK67_003147"
     CDS             189..2234
                     /locus_tag="KPK67_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2460074.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            2495..3694
                     /locus_tag="KPK67_003148"
     CDS             2495..3694
                     /locus_tag="KPK67_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG2460075.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            3746..4441
                     /locus_tag="KPK67_003149"
     CDS             3746..4441
                     /locus_tag="KPK67_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG2460076.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            4455..5132
                     /locus_tag="KPK67_003150"
     CDS             4455..5132
                     /locus_tag="KPK67_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460077.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKISD"
     gene            5243..6843
                     /locus_tag="KPK67_003151"
                     /pseudo
     CDS             5243..6843
                     /locus_tag="KPK67_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
BASE COUNT         2810 a          693 c         1312 g         2192 t
ORIGIN      
        1 ccaataatgt caaactaacc gttttagaaa ttaaattata tgtgaaaaaa gatagcttac
       61 attattttat tatggaaaat aatagtaaat ggtacaaaat ttgattttta agatttaaaa
      121 cttttttaaa aagtgctaca atagaccaaa ttattacttt ctatactcgt taaacaggag
      181 gtgggactat gaaaaaacat aatattttat ttgtatcaac tgatgacaaa ataaatatag
      241 atatatcaaa acaattggaa aatatatttg gagaattttg taatatagat aacttggtat
      301 atgtaaatag gataaatatt gaactgtcaa gctatgagct tgtagtatgt tcagacaatg
      361 atataaagga atatatacat aataatattg ataaaaatat acctattgta atagtacatc
      421 gtactataaa tattgaaaat ataaatcaga taataagtat agaaaatgat tctgatgtta
      481 tggttataga tgcttataaa gaatcagcag atgaaactgc caagataatt agaaaactag
      541 gattaattca tattaatcta ataccatatt atccaggttg tgataaatct aaatgtgaga
      601 ttggaattat tacgggaagc agaaacagca taccacaaaa cataaagcaa ataattgaca
      661 taggtgataa ggtaattgat ataaatacag ttatagaaat ctttacaaaa ctaaatatat
      721 ctatagataa actacatatt attaaggaaa aatacaatga agatacagta agtggatata
      781 ggtattatac aactatgaat aaaaccatga agagtttttt ggagattata gatgaaggta
      841 ttgcttctgt tgataaatta ggaaaattta tatattgcaa caaagtattt tctaatctag
      901 ttggaataga ccaaaatgaa attatatcta ataactttat ggacttattt agtgataagg
      961 ttgtcaaaaa aatatttttt caagaagatg aagttaatga tgaagtagta aatttaaata
     1021 acaaaaaact gataattaat aaagtaaatg tttatgaaaa taatgaacga ataaagagta
     1081 ttataagcat taaaaatata agtgcaattc aagtgcttga ggacaagatt caaaataaac
     1141 ttcagtcaaa aggttttgta tcaaagtata catttgagag tgttgtaggt gagagtaaaa
     1201 taataaaaga aaaaattaat attgcaagaa aaatagcaac tacagacttt tctgtgttga
     1261 tacttggaga aaatggaaca ggaaaagaaa tatttgccca ggcaatacat aatgaatctc
     1321 taagaaaaaa caagccattt gttgcagtta atctatcttc tttgtcagat acacttattg
     1381 aaagtgaact ttttggctat gatgaaggaa gttttacagg agcaataaaa ggtggaaaaa
     1441 tgggtatatt tgaaagagca catacaggaa caattttttt agatgagata ggtgatatat
     1501 ctttagatgt acagcagaga ttgctaagag tattacagga aaaagaagtt atgagaatgg
     1561 gcggaagtaa aattatacca atagatgtta gaattatagc tgcaacaaat aaagatttaa
     1621 agaaaaaaat taaagaaggt tcttttagag aagatttgta ttataggatt aatgtattac
     1681 atatagagat acctagactt agagaacgta aggaagatat acctcttata agtaagtatt
     1741 ttttagatga aataaatagc aacaaatgtt ttacagagga atcaatgcaa gctttaacat
     1801 tgtatgaatg gccaggaaat gtaagagagc ttaaaaattt aatttattat attgatacta
     1861 tagttgagga agatagagta gattacgagc atttgccaga acaatttaga tttgaaaaaa
     1921 acaatacttt agttaatgaa aatttcgatt caattatctt agatttcaaa caatctaatt
     1981 tctttgagga aagtatatgt atattgacca gtatagaaac ttggaataat aaaaatatat
     2041 tattaggaag aaataaactg caagagattt taaaagaaaa agatatagtt ttaagtgtag
     2101 accaaattag aaaaagaata gataagttaa agagccatgg tttactttta tctggagtaa
     2161 aaaaacaagg tagttttata actgatgaag gaaagaactt tatatcatat gtaaaaatta
     2221 agggagtgat ataggggact aaaaaggcaa aacaccctaa aaaaatccct ataatggata
     2281 gaaataaata aaattatctt taagattaag ataattcaaa aatattgaat aaataatcta
     2341 agaagattat ttttaataaa acagtagtaa atatcaacct tactactgtt tttatttttt
     2401 gcaagattta ttatacaatt aaaatttata ttttggcaca tcaattgcta cataggaaaa
     2461 atataaaaaa ttgtagaact tggaggatgt tgttatgaag tcagcttaca aaggatatga
     2521 agcctatagt tatttagaaa aaggaaaaga tatttcatct attgagatgt gtaaaggaga
     2581 taaaagagtt aaagagtacc ttattgaatt agatgatatt caagaagcta aggttaaaag
     2641 tatagtggat gaaaaaatat ttatatcatt acatgaacat ccagtattat ttccagaaca
     2701 cctagaaaga gatatatttg aatataatag ggaaggtaaa caaagatgcg cttatgaagc
     2761 attatcaaga ggatattatg atgctatatt tgataattta atggatggtg tatgtacgat
     2821 aacatcaaat tcagggtgga aatgggatga tatattaata gatttaggca tgagactatg
     2881 tgatttagct catcaagatt ttgttataaa gtgtgaaaaa gtagaagata tatacagagc
     2941 taaaagtgaa ggtaaagtag cattgatacc aacattagaa ggtgcagcac caatagaaaa
     3001 tgaacttgac agaatagata tactgtatgg atttggtata aggttaatgg gtataacata
     3061 cagcgaatcc aatgcattag gttcaggact aaaagaagag aaagatggag gtcttacaaa
     3121 ttttgggaaa aaagctgttg aaagaatgaa taaaattgga atggcaatag attgctctca
     3181 cgttggagtt caaactacac ttgatgtaat agaatatagc aaggacccaa taatattaag
     3241 ccatgttggg gcaagaagtt tatggaacac aaagagacta actccagatg aagtattaat
     3301 agcatgtgct aaaaaaggag gggttatagg tgtagaagca gcacctcata ctacaataac
     3361 agagaagaat aaagaacatt caatagagtc atttatggaa cattttgagt atattaaaaa
     3421 tttagtagga atagaccatg tagcttttgg accagataca gtatatggcg accatgtaaa
     3481 attacactca gtactagctg ctaaatttga tataaaaaaa ataacaggaa caaaagaaca
     3541 tcccaaagta gaatatgtaa aaggtcttga gaacccaaca gaaacttcaa aaaacattgt
     3601 tagatattta gtaaagcatg gatatagtga tgaggaaata gagaaggtac taggtggaaa
     3661 tatacttaga gtactaaaac aggtttggaa atagatatat tagaaatatt aataaaattt
     3721 gaggatttat agggaggttt tataaatgga ttatttaaaa ttagctaatc atccactttt
     3781 gtggatagcg agtacagtag cagtatcaat agtagtggta caatccctta tatttgctat
     3841 aaaatcttgc aaagttggta aaacaatggg aataacagat aaacaaataa agtcagctgt
     3901 taagagtagt gctatttcag ctattgggcc ttcaatgact atatttgctg gaatggtatc
     3961 gcttatagta actatgggtg ggcctatagc atggatgaga ttatcattta taggttctgt
     4021 aatttttgaa tcaatgtcag ccggatttgg aacagatgca ttaggtataa ctttaggttc
     4081 tcctgaaatg acaaaacttg catttacaaa tgcagtttgg actatgatat taggttcttt
     4141 aggatggata atattcacat tactatttgg acataaacta gataagataa ccaatgttat
     4201 atctagtggc aagaaatcat ttataccaat aatatctcta ggagcaatgt tgggttcatt
     4261 tgcatatcta aatgctgata gggtgttgcg ttttgataat ggaacaattg catgtataag
     4321 tggaatggct ataatggtaa tattgtgtat gcttgaaaag aagaaaaata taaaatggtt
     4381 aagagagtgg gggcttacaa tatcaatgtt tagcggaatg ataatagctt cagtaatata
     4441 agggggggag ttttatgagt aaagtaaaat ctatatacaa tgaagaatat ttacctttta
     4501 tgataaggta tggaagacta acactatctt taggtattat agcagcatta gttcctggaa
     4561 taattttgag ctttggattt ggtattatgc ctccaatatc agcattatta gcaagtacaa
     4621 tggcaattgt cagtatgagt gctccaaatt acataataga accagtttct tattcaccaa
     4681 tcttaggtat cccaggaact tatatgagtt ttctttctgg taatatatct aatatgaggc
     4741 taccatgttc aatagcagct caaaaggcag cagaagttga atcaggtaca gaagaaggct
     4801 cgataatatc tacaatagga atagctgtat ctattttagt gaatataagt atactaacta
     4861 taggggtaat tttaggagga agcgtattat ctaaaattcc agctgaagta gtagaaaaat
     4921 taaacttaat tttaccagct ctatttgggt cagtttttgg acaagtattt ttacaagata
     4981 aaaaattagg tctagtagca attgtaatat ctgtattgac tattatatta tcaaagcaag
     5041 gtataatacc acagtctcta gtagttctta tatgtgtatt tggtactata ttaattgcaa
     5101 gagctatgta taaagataag attagtgact aattacaatg aaataatagt gaattttcaa
     5161 atatgaaata taataatttg attttcaaat ataaaaatta cattaagatt atttaaaaat
     5221 tggtttttaa ggagggaata ttttgatata tgatttaatc ttaaaaaatg gatttataat
     5281 agatggtaca ggtaatcctg gtttttatgg agatatagca attaaagaca acttaatagc
     5341 aaaaatagat tctaagataa atagcaatac aaataaagag attgattgtt gtggaaaagt
     5401 tataacacct ggatttattg accctcatgt tcatgaagaa atagtagcaa tactagatgg
     5461 taaatttgaa aagtttctaa aacaaggagt tacaacaact ataaatggta attgtggaca
     5521 ttctataaca ccatattctt ctgaaaatgt gtatgaatat atgtataaaa atggtctatt
     5581 acttgaagaa gaaaaaaagt atcttataga taaaaataaa tgttggaata attttacaga
     5641 atattgtgac cttatatcta aaagtggtat ttctgtcaat atgggattct tgttggggca
     5701 tgggactata agatggagtg ttatgggagg ttctaaggat agacctccta cagaaaagga
     5761 aaaaatgaaa ttacagatat aataaatgat ggaatgaaaa gtggtgcttt tggaatatct
     5821 actggattgg catatatacc aagtaagtat gctgatattg atgaacttgt tgatattgca
     5881 aggcagatta aagagcatga tggtatatat acatctcata ttagagatta cataggaaga
     5941 tataatgcag taaaagaagc catagaggtt gggcaaaaaa gtggtgctag ggttcaagta
     6001 tctcacttaa gtccagttga aatagaagca tttgatgaga tattaaaggc taggtataat
     6061 ggtgttgaaa taatggttga cacagtacca agaagttctg ggcattgtat gaagaaaaaa
     6121 agagtaattc aatttattat ggcaatatca tctagtctat ttgaattagg tatagatggg
     6181 gtaatggatg ctcttagaaa tgaagaggga agaacactta tattaaaaga agcatttata
     6241 cttggagata gggggagtat aatacttcta aacactaaag atattaatat ggaaaagaaa
     6301 tcaatacgtg aaatagctac acaaaaagga atagatgaag acaagttatt actcgattta
     6361 cttttagatg gagacgaaga acttatcttt tgtttaggag gaatgtatag agctgatttc
     6421 ccagataaac tacatgatga taaaataatt gataatccat ttgttatggt tggttcagat
     6481 tgtttgttta gtgtgggtgg ggatatgtca tggtttgaac tacaaagaag aggagctttt
     6541 ccaattttct tcaatatgta cagaaagtct ggtgttagat tggaagagat tgtaagaaga
     6601 gttacttcat taccagctag gcaatttaaa ataaaaaata gaggtattct taaagaaggg
     6661 ttgatagctg atatagcagt tattgacatg gataactata gttattctag gtcagaagat
     6721 attgattttt caaagccaca attactagcc aatggtgttg aatatgtaat tgtaaatgga
     6781 aaaattgcgt tagaaaatgg agagattact gagaataaat gtggtgaagt attgaaaaga
     6841 taaggtttta aaaataatag gatttagaaa gtaaataaat atctaagtaa gtaaagacga
     6901 agtttgagat ttatttaaat aaaaatcatg cttcgttttt atttatagta ttaatattta
     6961 ttttaataat cagataatat ttatacaaaa aatatttata ctttata
//
LOCUS       DAEMHW010000187         6935 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEMHW010000187 DAEMHW010000000
VERSION     DAEMHW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.187"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(141..524)
                     /locus_tag="KPK67_003152"
                     /pseudo
     CDS             complement(141..524)
                     /locus_tag="KPK67_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein MotB"
     gene            complement(545..1333)
                     /locus_tag="KPK67_003153"
     CDS             complement(545..1333)
                     /locus_tag="KPK67_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBG2460078.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSELVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(1421..1511)
                     /locus_tag="KPK67_003154"
     tRNA            complement(1421..1511)
                     /locus_tag="KPK67_003154"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1475..1477),aa:Ser,seq:gct)
     gene            1770..2534
                     /locus_tag="KPK67_003155"
     CDS             1770..2534
                     /locus_tag="KPK67_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG2460079.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(2815..3930)
                     /locus_tag="KPK67_003156"
     CDS             complement(2815..3930)
                     /locus_tag="KPK67_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG2460080.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIQIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLIFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(3971..5497)
                     /gene="glpK"
                     /locus_tag="KPK67_003157"
     CDS             complement(3971..5497)
                     /gene="glpK"
                     /locus_tag="KPK67_003157"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG2460081.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTNQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            5790..6887
                     /locus_tag="KPK67_003158"
     CDS             5790..6887
                     /locus_tag="KPK67_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG2460082.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMNLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
BASE COUNT         2310 a         1233 c          882 g         2510 t
ORIGIN      
        1 gttagcttta ggggtttcca gcaatccaag tagtattgga taggttctat cctatctcta
       61 catataggtt tccctatatg ctgactattt tagctactaa ctcatgacta aagtcacgag
      121 tgtgcgtagc tattttttta atcaaatagt attgtatctt taaggcttat tactaatcct
      181 ctatcttgaa ctcttaatga tactgaatta gccataccat tttcgtttaa atacttctgg
      241 atttcttcac caactttttt aaacttagca ttttgtaaag tatctaaatc attacttcct
      301 ggttcaacta ctggttctat cttgctatta ccaccttcaa taactccaga agaaccacca
      361 aaagcagaat ttagagattg agataattgt ttatatttct ccgcatcaac attactcata
      421 gaatacatta ttacaaagaa aatcataagt aatgttatga ggtctgaata agttaaaagc
      481 catcgctcat tgttttcttc tttttcatct tcatcataca acataaacat tccatccttt
      541 tatattattc agctctacca ccaatttctt caagttcaat tagttctttt gaatttaaaa
      601 atatttttaa tttttctgtc attaaattag aattatcacc ctcaagtatg gataatattg
      661 cttctatcac caaaagtctt tcacttactt cttgttgatt tatagcctta agttttgatg
      721 ctattggtaa ccatattaaa ttggcagaac caacaccata caaagtagcc aagaatgcaa
      781 cagaaatttg aggtcctaat gcatctggat tacttgaaag attacctaaa atatgtacta
      841 atcccataac tgtacctatt atccccagtg taggtgcata tccacctgct gaatcaaaaa
      901 ttgctattcc ttctttatgt cgttttgatg ttatctgtat ctgtgattcc aatatatttc
      961 ttatagtaac tgcatccact ccatctacca taagttcaag cccttttcta ataaaagggt
     1021 ctaattcatc attctcactt atttctttgt caatactaag aagcccctct ttcctagcct
     1081 ttgttgcaat ttctttgaaa tagattatat tttctatgtt attaggcttt ttatttttaa
     1141 aagctaataa cattatcttt atagactttt taaacacagg gaatggagtc gaagccccaa
     1201 cagctcctat agttccacca aacacaacaa gttctgatga aaatacaaat aatgctccaa
     1261 catgtccccc atcaataaca aaagcaacta ccaaagatgc tatccccact atgaaaaaaa
     1321 ttaaagttgt catctattcc ctcctttctt tagttataca tatctaatca tttatgacat
     1381 tataaaaaga ctatctttaa taaaaatagc ctttaataat tggcggagaa ggagggattc
     1441 gaaccctcgc accggttacc cagcctaatc ccttagcagg ggatcctctt gagccacttg
     1501 agtacttctc catatctcta tactaataaa ttatataata aaatcatatt ttagtcaatt
     1561 gttttataaa atttgagtta ttttgaatta ttaaacatct atttttattt ttttgcttaa
     1621 atgtaagttt taaatatggt aaaattatct tgcataacac ttataatatt atttgataaa
     1681 tatacataca ttaacaaata tacctaaact ctgacttaat tgtaagttat gccaaaacta
     1741 aaataatact agtccaagga ggatatttta tgttaccaca ggaaagatat gagaagattt
     1801 taagtattct caaatcagat aatataatta aaataaatga catagttcaa atgtttgata
     1861 tatcaataga aactgcacga agagatttag cccatatgga gagtatcggt cttttaaaaa
     1921 gagtttatgg tggtgctata ccagttatag caagtagcgt agaacttaac tatgttaaaa
     1981 gaaaaaaaat aaactcaaac gataaaaaac agattgctat taaatgtagt gagtttataa
     2041 atacaaatga tactattttt atggatactg gaactactgt acttgaagtt tcaaaaaatt
     2101 tgaaagataa aaagaatcta actgtaataa ccaactcaat tttagttgca aacgaactta
     2161 tgaatactaa tataaaagta tatgtgcttg ggggctatct tagagaaact gaaggttctt
     2221 tttctggtcc gcttacactg tcagctattg aacaatttaa tgttgataaa gctattttag
     2281 gagctggagg tatcactctt gaaaatggaa tctctgacta tcacttagaa gaagcacttg
     2341 ttagaaaaaa aatgattgaa aggtctagaa aatctatagt agttgtcgaa cctcctaagt
     2401 ttggtataaa ttcatttgca tcagtagctc ctatagaaca tatagatact attattacct
     2461 ctgctagtat agaccataat ataataatag agttttctca aaaaggaata cctttagtca
     2521 tagctgataa ttagcttata tgattgattt ttagttcctt gatgtaataa attaaaatta
     2581 aggagccaaa taattattta tttagctcca tttatgtcta aactatactt tatattttaa
     2641 ttatgtaact actttcagtt agcttatcat ttttagttat ttgattgcca ttttatttaa
     2701 tttataagtt ttagttattc gattattttt taattgtctg tctttttaat ttatttttag
     2761 ttatttaaac tcctacttta aaataattta aatttgactt aagtattgtt tattctacac
     2821 tgcatctttc ttcattttaa tactttgttc ttcttccaag gttaaatctt ttgtcataag
     2881 tatagcaagt gcaattgcca atactcctcc aaccaatttt ccaattacaa caggtgtaat
     2941 catctctgga actactcctg ctgtaaatcc taaatggtcc cccaaagctg ctgtagcaca
     3001 aaccagccaa gcagtattta ctactttccc ctttggactc atatctttca tcattttata
     3061 tactggaata ctattggcta aagtaaaaac taatcctgct gcactagtgg catccatacc
     3121 taattttcca cccactattg taagtggttt attaagaact ttaactaata aatgtaatat
     3181 tggaaaagta cctaataaca cgattgcaat acttccaatt acttgaatac cttctcttat
     3241 aggtgtcatg ccttttataa gtactatacc tgttatttct tgaaatgctg ctgctcccaa
     3301 acctatcgtt atcaatatag atataaattt tccaaaaatt aatgacccat ttatcataag
     3361 cgtagaatta aattttaaac ctactgccaa tattactgcc aatattatca ctggtatcat
     3421 atttataatt accattgata agttaaatcc tgctattaat cctcctataa tacctccaaa
     3481 aggtattgtt atcaagccta tcagtaaccc ttttgcaaag aatggtctgt cttcatattc
     3541 tataagacct agtcctactg gaattgaaaa tactaatgta catccaagca tggatgacac
     3601 aaccaatcct gcaagcattc ctgcctcttt attgtgtgca agttccatag ccaatggata
     3661 ccctcccata tcattcgcta aaatagttgc aaacatagat gggtcagctc cacagagatt
     3721 gaagatttgt attattatag gacctagtac atctgaaatt actggagcta aacaaactat
     3781 accaaccatt cctaaagcca atggacccat tgaattaaac ccttcttcaa actgctctcc
     3841 aagtccaaat ttatttccaa ttattctatc taacgctcct aaaagcactc ccacagccat
     3901 tatgtataat ataattttat caaagggcat ttttaattcc tcctatacat gtaaaatact
     3961 ctattgtaac ttaattagca cacacctttt tggtatcgtt ttcccaattc atagctcttt
     4021 ttactgcttg attccatcca agtaatattt cttctctttt ttgattagtc atatttggaa
     4081 tatatttttt gcttattgta aattcacttc taatttcatc aatactgttc cagacaccac
     4141 aagaaagacc tgccatatat cctactccaa gtactgtcgt ctcaggattt tctgcaattt
     4201 caattggtat acctaatatg tcggattgaa attgcattgt aaagttattt ttagtaattc
     4261 caccatctac tctcaacatt tttatttttt tccctgaggc taagttcata gaatcaacaa
     4321 catctttgac ttgaagtgca acagactcaa gagtcgctct tactatatgc tcttttttag
     4381 tacttcctgt tattccaacc attgtacctc tagcatattg gtcccaataa ggtgctgcta
     4441 tacctgcaaa tgctggtaca aaatatacac ctccagtact tgatatggaa tttgccatgt
     4501 catcagtttc tgaataattt tttataactc caatgccatc tctaagccat tgtattgcag
     4561 cacctgctat atacactcca ccatcataag catatgtaac tttatctcct attttccaag
     4621 caacagttgt caaaatacca ttacctgcta tatttaattc ctcacctata ttcatatata
     4681 ggaaacatcc tgtaccataa gtatttttaa ccattcctgg ttcaaaacat ccattgccaa
     4741 ataaagctgc tggttggtct accattgaag cagtaatttt tatagaaacc ccacaaaatg
     4801 cctccttatc tgtagttcca aaatcaccac tcgtaggcat tatatctgct aaaatacttc
     4861 taggtactcc tataatgtca agcaactctc tatcccaatc catatctctt atattaaaaa
     4921 gcattgttct tgctgctgta gatacatcag ttaaaaaaga ttttcctcct gtcattttcc
     4981 atattaacca actatctaat gttcctgcaa gtacttctcc atttttaatt ttttctttta
     5041 caccttttac attatctatt acccatttaa tttttgtacc tgagaaatat gggtctataa
     5101 ttaacccagt cttatctatt atcttatttt ctaaacctgg tatattcttt aaattttcca
     5161 cataatcata agttctcctg cactgccaca caatagcatt gtaaagtggt ttacctgtat
     5221 ttttatccca taacattact gtttcacctt gattatctat accaattcct tttatatcac
     5281 tttcattgat tccattttca aaagcctgtt gtaatacatt attactcaca gtttttgtat
     5341 tattccaaat ttctataggg tcatgttcaa catatccaga ttgtggatat atttgagtat
     5401 gttccatata atcgctaaaa acaaactctg atttttcgtt tatcaatact gctcttgttc
     5461 ctgttgttcc ttggtcaatt gctaatacat atttcacttt aaatgtcccc ctttattttc
     5521 agaattaata aattcttttt tattttttga atatataatc tattcaagtt atttatattt
     5581 tatatcatca tgtggattta tgcaatattc attacaatat tttttgttat ttttaattat
     5641 agtgattaaa aaagtatttt tattgaatta ttattgcaac atttattatt ttattctata
     5701 atctagttaa aataacacat aacattttaa tatttacaca taatcagtca ttcaaaaatt
     5761 taaatttaac tttttaagga ggctttacaa tgaaaaaaac atatctattc agtcctgggc
     5821 cagtaatggt tacagataaa gtcagaaatt cacttcttca ctatgatata tgccatagag
     5881 gaaatgagtt tatgaattta ttcaaagata cacaacaaaa aatcaataaa ctatataatg
     5941 caacttctaa ttactactca ttaattgtat ctggctctgg aacatctgta aatgaatgtg
     6001 tactatcttc tatctttgac aaagaagatg cagttctgct agttagtaat ggtgtttttg
     6061 gagaaaggct agaagaaata ttatctactt ataatgtaaa aacatataaa cctaattttg
     6121 aatgggcaga atacccggac ctagacattt tagaaaaata cataattgaa aattctgata
     6181 taaaagtaat tgctatggtg ttccatgaaa ctagttcagg aatgataaat ccagtccctg
     6241 aactaggaaa acttgctaag aagtataaca aaatattttt tgtagatgca ataagtgcct
     6301 cagcaggtga atatattgat gtggaagagt ttaatataga tataataaca ggtgttggtg
     6361 gcaaagctct tggagcattt ccaggttctg catacctttg tgctaaagag agtattttac
     6421 aaaatattaa agaaaatcaa tgtaagaatg tgtatcttaa tctttataaa cactataaag
     6481 ttgctaaatc atcttctcaa acaccaaata cacctaatgt tactttaata tttgccctta
     6541 atgaagcttt aactgagttt ttggaagatg acagtaaaat agaaagatac aaagaatgtt
     6601 ctgctatttt aaggaatggt atggaagaat tgggtctaac atttttactt ccagataagt
     6661 atatgtctaa tacagtaaca tctgtatttc tacctaaaga aatcaatgta aatgagttta
     6721 tattagggct agaaagggaa agtggctatg ttgtctatcc aggaaaaggc aaattcctag
     6781 atgctaatat attccaagta gcaaatatgg gagaaatata tccagaagat tgttataaat
     6841 tcttagatat cttaaaatca aaattggaga taaaaaaagt tatataacag tttgtttgta
     6901 aaaccctaca atatatcagt ataaattaaa tagac
//
LOCUS       DAEMHW010000188         6933 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEMHW010000188 DAEMHW010000000
VERSION     DAEMHW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.188"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1031
                     /locus_tag="KPK67_003159"
     CDS             <1..1031
                     /locus_tag="KPK67_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG2460083.1"
                     /translation="ELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTMHQMKS
                     NVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLERKYSK
                     RESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTIDFIIAF
                     QEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIRELEHD
                     DIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIKQSVLC
                     FARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDILSKCDI
                     EKVKDILI"
     gene            1172..1480
                     /locus_tag="KPK67_003160"
     CDS             1172..1480
                     /locus_tag="KPK67_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2460084.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            1504..1830
                     /locus_tag="KPK67_003161"
     CDS             1504..1830
                     /locus_tag="KPK67_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2460085.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            1869..3203
                     /gene="celB"
                     /locus_tag="KPK67_003162"
     CDS             1869..3203
                     /gene="celB"
                     /locus_tag="KPK67_003162"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBG2460086.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINVSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            3214..3732
                     /locus_tag="KPK67_003163"
     CDS             3214..3732
                     /locus_tag="KPK67_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBG2460087.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            3828..4787
                     /locus_tag="KPK67_003164"
     CDS             3828..4787
                     /locus_tag="KPK67_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBG2460088.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIERRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            4836..5888
                     /locus_tag="KPK67_003165"
     CDS             4836..5888
                     /locus_tag="KPK67_003165"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBG2460089.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            5891..6565
                     /locus_tag="KPK67_003166"
     CDS             5891..6565
                     /locus_tag="KPK67_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBG2460090.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQRDIGVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
BASE COUNT         2811 a          697 c         1299 g         2126 t
ORIGIN      
        1 aagaattagc aagttataca aaaaaaatta taaatttagt atcagaagaa tcaggaataa
       61 attttaataa tgatatatta ctttatgaac aattaatatg tcatttgaat gttacaatgc
      121 atcaaatgaa aagcaatgtt tacttagaaa atccactttt agataacata aaaactaaat
      181 tttgtttttt atttacagta atatcaagtt caatagaaat tgaattaaaa ggaaaacaaa
      241 taacggaaga tgaaattggt tatcttacac ttcactttca aacctctcta gaaagaaaat
      301 acagcaaaag ggaatcaaat aaaaaggcaa gtattgtatg tccatttagt tttggagtaa
      361 gtatgctatt aaaagtaaag atagaaaaga gatttgataa cataaaaatt attgaaacat
      421 taagagagga agatttaaaa agagataact ttaataagac tatagatttt ataattgcat
      481 ttcaagagta tgaaaatatc caaaaaccaa tttttataac aacaccatta tttactgatg
      541 aggatgagaa taaacttaaa gagttcacaa acaaaataaa agaaaaagac acttcatata
      601 aacttatgaa cagattaatg atgtctgatt atttaataag agagttagaa catgatgata
      661 tttatgaagc tataacatat ttaactaatt tattaattga aaaacattat gcagaaaaag
      721 agtatttaca atctattata acaagagaaa agtcatatcc aaccaatgtt ggaaaaggaa
      781 tattattccc tcatggagac atgaaatata taaaacaatc tgttttatgt tttgcaaggt
      841 taaaaacacc aataaaaatt agaaatggaa aggatataaa gattatactt ttgttagcat
      901 ataaaaaaga tgatgataga aaagtattta gagaattatt taaagaaata tctaatttaa
      961 cagaggatga aaatatgcta gatattctaa gtaaatgtga cattgaaaag gtaaaagata
     1021 ttcttatata aaattcatag tgaactatgg ataagcacaa aattttccat gtgatggaaa
     1081 aagttgtgca tgtttttttg tttttataag atattatatg tttgtaaggt ataaataatc
     1141 taaaagtaga gaagcaactt aggaggaaaa aatggaagaa atagttttaa agattataat
     1201 tcatgctgga aatgcaaaat ctatgcttta tgaagcactt gactatgcaa aggaaaacga
     1261 ttttaaaaaa gcagatgaat taattgaaaa tgcaaatgaa gaaattttaa aagcacataa
     1321 agtacaaact gaacttatac aaaaggaagc tgggggagat aaatcagata tatctatttt
     1381 attgattcac tctcaagacc atttaatgac ttgtatgagt gagagaaacc ttataaaaga
     1441 aatgattatg ttaagaaaag aaattcaaaa attaaaataa aaaaattaag ggggatttaa
     1501 aaaatgaaaa gaaaagtata tttattttgt agctttggta tgtcaacaag tttacttgca
     1561 gataaaatgc agaaggtagc agatgaacac aatttaccaa tagaagtaga ggcatttccg
     1621 atagcagaaa ttgacaaaat agttgaagaa aaaaatccag attgtatatt attaggacca
     1681 caagttaaat acatgctaaa ggagttaaaa ccaaagtttg aagctcaagg aaaacttata
     1741 gatataatca atgaagttga ttatggaact atgaatgggg aaaaagtatt aaaacttgct
     1801 ataaagctaa taaaatctaa acaagcgtag aattatagta aaaaataata ggaaatgagg
     1861 gtggatagat gaatagattg gaaagaatac ttatgccatt ggctgaaaaa ataggtaaaa
     1921 ataaattatt aattgcaatt agagatggtt ttttagtatc atcaccatta ttaataatag
     1981 gttcattatt tttattagta gcaaattttc ctataaaagg atggaatgaa tttgtaggtc
     2041 aaatttttgg tccagattgg gctgtaaaat tgcaacaacc aactgtagca acatttgaag
     2101 taatgactct acttgcagtt ttagggattg gatattctta tgcaaagcaa atagatgtag
     2161 acccaatagc atcagcagca gttgcaatag ttgccttttt tataataaca ccatttgtaa
     2221 taccatatac accagaagga acagaatcag tttacatggt aacaggtata ccactaggtt
     2281 ggatgggttc taagggaatg tttgtaggta tgataacagc aataacctct gtaaaattat
     2341 ttgctgcagt tgtaaaaaaa ggatggacat tgaaaatgcc agatggagtt cctccaactg
     2401 tatcaaaatc atttgcagca ttaataccaa gtgcaatagt tatggtagtt ttctttttag
     2461 ttaagatagc ttttgaagct acaccatatg gaagtataca tgaatttata tttaagtttt
     2521 tacaaatgcc attattaaaa ttaggaaata gtttaggtgc tatggcaatc gcttatatat
     2581 tcttacattt attctggttc tttggaataa atgtaagtag tgttgttggg gctgtgtata
     2641 atccaatatt aaaaattcta tcagctgaaa acttagctgc ttttcaagct ggttcaaaaa
     2701 taccaaacat aataacaggt caattccaag atatgtttgc gacatttggt ggagcaggta
     2761 gtacactatc attagtcata gctatgttat taatatgtaa aagtaaacgt ataaagtcaa
     2821 tgggaaaact atcaatttta ccaggaatat ttggtataaa tgagccattg atatttggtc
     2881 taccaataat gcttaaccca atactattga taccatttgc aatagttcca acaataaata
     2941 taataatagc ttacttctgt atgtcagcag gattagtgcc tcttacaaat ggagttcagt
     3001 taccatggac aactccaatt atattctctg gttttttaac aacaggatgg caaggtgcag
     3061 tcttacaatt agtacttctt atactaggaa tatttatgta tataccattt ataaaaatgc
     3121 tagataagca atacttgaga gaagaaaatg aaactgttga agaggaagat gatgatatat
     3181 cttttgatga tttagtatta taggaggaag attatgaaga aaatagttat gatattggac
     3241 caaatacaag ctggagctgg aggaaaagag aaaagtaata tacctcctgc tggaaaatca
     3301 agcccattag gaccaggtgt aatgatggag caatttttaa atgaatctaa ggtaatagct
     3361 accttatttt gtggggatga attttttgta aataatcaag aagaagtaac gagtaagatg
     3421 atagctatgg taaaaaaatt aaaccctgat gtagtaatct gtggaccaag ctttaattac
     3481 gaaaactttt ctaagatgtc agcaatactg tctaaaaata taaatgataa aacagatata
     3541 ccagcttttg cagctatgtc agaagaaaat atagatgtga taaacgaata taaaaatgac
     3601 atatgtatag ttaagactcc aaaaaaaggt ggaattggtc ttaatgattc attaaataac
     3661 atatgtaaat tggcaaaagc aatagcaaat aaagaagata taactttgat gaaagaagaa
     3721 ttttgttatt aataagagta ttaattatta gcaattttaa atattatact atttataata
     3781 agcatacatt agtaaaatct atataattta aggaggggaa ttttaacatg tggggaatga
     3841 tagcgacatg gagaatggcc ctagaaggaa taactgaggc atcagaacat ttagaaaaag
     3901 gcatggaagc aggtgatgct gtagagcttg ctgtaagaag ggttgaagac tatccattct
     3961 ataaatcggt tggatatggt ggacttccaa atgaaaattg tgaggtagaa cttgacgctg
     4021 catttatgga tggtaacaca ttagatattg gcgcagttgc tggtataaga gattattcta
     4081 atcctgtcag catagctagg aagttaagtt atgagaaggt aaataatttt ttagttggaa
     4141 taggtgctga agattatgct cataaaaatg gatttgaaag aaaaaatatg ttgactgata
     4201 gagcgaagtt acattataaa aagagaaaaa aagagacttt agacaaagga ttaagtccat
     4261 atgctggtca tgatactgtt ggtatgatat ctttagacaa aactggaaaa atgtgtgcag
     4321 ctacatcaac aagtggattg tttatgaaga aaagaggtag aataggagat tctgcactat
     4381 ctggttcagg attctatgtt gatagtagta taggaggagc tacagctact ggtttgggag
     4441 aagatttaat gaagggatgt atatcttatg agatagttag acttatgaaa gaagggaagc
     4501 atccacaaga agcatgtgat attgcagtaa atgaacttga taaacagctt atcgaaagaa
     4561 ggggaaaagc tggtgattta tcattagttg ccatgaataa taaaggtgaa tggggagtag
     4621 ctacaaatat agctgatttt actttttcag tagtaactaa agaagaatct cctactgtat
     4681 atatttcaaa tagagtagat ggaaagacta tacataaggt tatatcaaat gagtggatgg
     4741 aagcatatga ggctagaata aaagcaccaa tagaagtaag tgagtaaagt agtggttaca
     4801 taattaaaat agagttttat aataaggaga tttttatgaa tatcataagt aataaaatag
     4861 atgagttaaa agaagattta ttaagtgata ttatagatat tgtaaaaata cctagtgtca
     4921 aaggggagtc agaaaatggc ttcccttttg gcgaaaaagt aggggaggca cttaataaag
     4981 cattagaaat atcagaaaaa ttgggattta aggttagaaa cttggataat tatattggtt
     5041 atgcagagca tggagatagt gatgattatg tttgtgtaat tggacatgta gatgttgtac
     5101 atgaaggaga tggttggaag catcagcctt ataagggaga agaaactaat ggaagaattt
     5161 atggacgagg agtactagac aataaaggac ctataatgtc tgccttatat ggattatatg
     5221 ctattaaaga attaaactta aaattagata agagagttag aataatattt ggtacaaatg
     5281 aagaaagtgg atttgaagat ataccatatt atttagaaaa agaaaaagca cctattatgg
     5341 gatttacacc agattgtaaa tatcctgttg tatatggtga aaaaggcatg gcaaagatta
     5401 gaataaaaag taaaatcaat tatgaagaag atgtatacct aggttttata gaaaatatgt
     5461 ctgaaaatgt gcttgttaca tataaagagc tgaatataga aaatagtgat actatattag
     5521 atataaaggt aaaatacgat tttagttata aattgaaaga tgtattagat gaaataaaag
     5581 ctagttttcc aaatagtatc gatatagagg taatttctaa ttttaatcct gtatattttg
     5641 ataaagagag taatcttgtt aaaaagcttc agctagctta tgaaagggtt acatcattag
     5701 atggaactcc cgtaacaact aatggaggta cgtatgcaaa agtaatgcca aatatagttc
     5761 cgtttggacc ttcatttcca ggacaaaaag gaatagctca taatcctgat gaatacatgg
     5821 atatagaaga tataatatta aatgcaaaga tatttgcaaa tgcaatatat gaattagcaa
     5881 aggagtagtt atgttagaaa taataggaat gtcagtagaa gacgctaaaa ttatagaaga
     5941 ttgtggtgca gatagaatag agttagttag tgctttaact gaaggtggat tgacaccaag
     6001 ttttggtctt atagaaagtg ttgttaacag tgtaaagata cctgtgaacg taatgataag
     6061 acatcatgcg aaaagttttg tatacagtaa agaagatata agtataatgc aaagagatat
     6121 aggtgtggtt aaagagattg gtgctaatgg agttgtattt ggtgtattgg ataaaaacaa
     6181 tagtatagat gagaaaaatc taaatgtttt attgaaatgt tgtgataatc ttgatgttac
     6241 atttcataga gccatagatg aatcaaatac aatagatagt gttaaaattt tgaaggatta
     6301 tgataaaatt acgaatatat taacatcagg agggaaagga agtatagttg ataacataca
     6361 aatgataaag aatatgatgc ttagttctaa tcacataaaa attcttttag gtggaggtct
     6421 taattttaat aatatagaaa agataaagga attaactaag gcaagtaatt ttcattttgg
     6481 tacagcgatt agaataaata acagtccttt tgaagatata gatagacaaa agcttaaaca
     6541 actcgtaaat ataatttcaa gatagtatat gaatataaaa tacttagttt aaataaagag
     6601 agtaagttaa ttataatttg ggtatatgaa tgttaatagg tattaaatat gaaattattg
     6661 ttgagtatgc tttataactt ttaagtagtt ttaaatataa ataatgaaag aaaagataga
     6721 ttatggaggt tttagagggt ttatatttat taaaaaatta taaaggtata ttggcatata
     6781 atacacataa tttcctaaaa ttaccatctt atgacatgaa ttcacatatt aataaacaat
     6841 taatggagat ttttgtggat agaaatagga ataaaaatag ctttaaataa acaaaaacaa
     6901 gagttattaa cagttttatc cacattatcc aca
//
LOCUS       DAEMHW010000189         6908 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEMHW010000189 DAEMHW010000000
VERSION     DAEMHW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.189"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            175..1026
                     /locus_tag="KPK67_003167"
     CDS             175..1026
                     /locus_tag="KPK67_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG2460091.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            1030..1500
                     /locus_tag="KPK67_003168"
     CDS             1030..1500
                     /locus_tag="KPK67_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460092.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            1513..2823
                     /locus_tag="KPK67_003169"
     CDS             1513..2823
                     /locus_tag="KPK67_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2460093.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            3035..3781
                     /locus_tag="KPK67_003170"
     CDS             3035..3781
                     /locus_tag="KPK67_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2460094.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSNHQITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     ECSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            4143..5684
                     /locus_tag="KPK67_003171"
     CDS             4143..5684
                     /locus_tag="KPK67_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG2460095.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            5719..6825
                     /locus_tag="KPK67_003172"
     CDS             5719..6825
                     /locus_tag="KPK67_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG2460096.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDASSKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
BASE COUNT         2442 a          920 c         1208 g         2338 t
ORIGIN      
        1 ttcgttgaca atgttaaatt tttgttttat ccttaattaa ggacatgtta ttgacatttc
       61 tgaaacttat ttgaaatagt tttttcattt tatttaggaa aacgtattat acttaagaaa
      121 acgtattttc tcatttcaga taagtaaaat atttattctt ttggggagga aatcatggaa
      181 attaataaaa aacattttat tcaagtattt ttaacttgct taatcttggt agtaatatgt
      241 gaatttatag ggcaaagaac tatagctatt ggaaaagcct ctatagtact gttccctatg
      301 ctttatgcag ttataattgg acttataata acaccagatt tattaggtaa aaaaattaaa
      361 gcacttaaaa aagtggttgg agaaaaagaa ataaatattg ctggtgaagt tgttggaatt
      421 gcactagtaa tgcttggtat aaaatatggt actacagttg gaccaaacat tgacaaaata
      481 attcaagctg gaccagcatt tatcgctcaa gaatttggtc atatacttgc accaatcgtt
      541 gcattacctc ttgcattaat gtttggaatg aaaagagaag ctgttggagc tactgcaagt
      601 ataagtcgtg aaccttcttt aggagttata tcagaaaaat atgggataaa ctcaccagaa
      661 ggtagtggtg tacttggtac atatttaatg ggaacagttc ttggtactat atactttagt
      721 gtactaggtt caatatctat ttatagtggt cttcacccat atgctttggg aatggcctgt
      781 ggtgttggta gtggaagtat gatgacagca gctgctggtt cattatctac catggtacca
      841 cctgaaatgg cagatacaat acttgcctat gctgcaacaa gcaatatgat gtctagtata
      901 acaggaatta tattcttagt ttttgtatcc ttaccattta ctaattttat gtataagatt
      961 ttagaaccta aattttcgag aaataaaaaa actaaagaaa ttaaatctac agaaggagag
     1021 tgctaataaa tgtttgctaa tttaaattta aagaaaagtt catatcaatt attggttatg
     1081 tctgttattg gagttataat tcttatagga caaagaatta gcgttggtac atctatagtt
     1141 acagctcttc ctggtatggt gatgctaata ttagctgcta tggcagctat gattataaaa
     1201 gatttatttc ctaaatcaat ctttccagct tttggatttg caactataat cggcctttta
     1261 cttagtatgc catatagtcc aacatctgaa gtattcttaa ctaacactaa taatattaac
     1321 tttatggcta taactactcc actactagct tttgcaggaa tttctgttgg aaacaaaata
     1381 gaagctttaa aagaaatgtc atggaaaata gttataattt cacttattgt atttacaact
     1441 atattctttg catgtgcgag tatagctcat atagttttaa gtattcaagg taaaatatag
     1501 gaggaattta ttatgaataa agaacaatta aaatctctag taataaaaac tatagatgaa
     1561 aatagagaaa aaatattaaa aataggtcaa ggaatataca aaaatccaga atatggatat
     1621 aaagaattta aaactacaga agctgtttca aacttttttg agcaagaatt aaatttatct
     1681 gtagaaaaaa atatagcagt tacaggatgc aaagcagatg caaattctag caagaaaggt
     1741 cctcatatat ctatacttgg agaattggat ggtatctcat gtaaagaaca taaagatgct
     1801 aatgaaatag gtgcttctca tacttgtgga cataatattc agatagctgg aatgttaggt
     1861 gctgctgttg gtttagtaaa aagtggcgtt ttagaacatt tagatggtaa agtatctttt
     1921 atggctactc ctgctgaaga gtttattgag ttggaataca gagagcaatt aaaaaaagaa
     1981 ggaaaaataa cttattttgg tggaaaacaa gaattaataa aaagaggtta ttttgatgat
     2041 atagacatat ctatgatgtt tcatgcttct gatttaggag aagataaggc tctagttggt
     2101 cctgagagta atggatttgt tggaaaaaag gttaaattta taggtaaaga atctcatgct
     2161 ggttctgctc cacatgatgg aataaatgct ttaaatgcag caatgcttgc tataaacaac
     2221 gtgaatgcac ttagagaaac ttttaaagag tctgaaagag ttagattcca ccctatcatc
     2281 acaaaaggtg gagatatagt aaatgttgtt cctgctgatg ttcatatgga atcatatgtt
     2341 agagctagaa ctataaatgg tatgattgat gctaatgaaa gaataaataa ggctttgatg
     2401 gctggtggaa tggcagttgg agctgatgtt gagataactg aaattcctgg atatcttcca
     2461 atattacgat acaaaagtct tgataattta tttaaaaaca atcttgaaga tttaggtcta
     2521 aaaggaaaag ttattgatgg tggagatttt acaggctctt ttgattttgg agatgtgtct
     2581 catataatgc ctactcttca ccctatgatt ggtggtgtaa aaggagctct tcatacaaga
     2641 gattttgaaa tagttgatga agatttagct tacataattc ctgctaaagc aatggcactt
     2701 acagttgtag accttctttt tgataatgct aaggatgcta aagatatatt agataccttt
     2761 actccagtta tgactaaaga agaatatcta acattcttag aaaaacacga taaaacttta
     2821 taagtctatt tttataatag ggtggtatct actgtaaaag ttataccact ctttgttttt
     2881 gtaacagtat acttcttttc ttttagtttt tagattaaac taaaatatat tatcctcctt
     2941 aaaactttta gagatacgtt gaaatatcag tagaactatg ctatattctt tctaattaat
     3001 ataaatttat attctaattc aaggtggtga aaaaatgttt aatgcaggaa atcgaattat
     3061 aaatacatgg ttgtacccta ttcaagatgg ctttgtcttg attgatactg gatatgaaaa
     3121 tggttttaaa catttcaaaa aacgaatttc taaattcaac ataaaaatca aagatattcg
     3181 atacatattt ctaactcatg ctcatgatga ccatgttggt tttctaaatc agattctaga
     3241 cgaatctcct aatacaaaga ttgttatgca tgataaagct ttagaaattc tatataaagg
     3301 tcaaaactct tttattggtg gttgtaccag ccgtactgcc tttctctttt gccaaattat
     3361 gaaactattt ggaaaaggtg agcatagttt ttctccactt aaacaagaat ttgaaaaaag
     3421 gtgtattctt gtgtctgaca agaatcgtaa agaaattgaa aaagaactaa atggaaaaat
     3481 tatagataca ccaggacata ctgctgattc gatttcacta ctaattgatg atggtgtttt
     3541 attttgtgga gattctgcaa tgaatggatt tccaagtaat caccaaatta ccatatttgc
     3601 agaaagcaaa ctagaattta ttcaatcttg gaaaacaatc atcaatacaa aacctaaaat
     3661 gatttatcct ggacatggaa aaccatttga atgcagtaaa ttagaacaaa atctaatcta
     3721 tgtaaataaa ataaatttat atcctctaca taattcaaaa gatattcaca ggtttttgta
     3781 gaaaatacaa agtaaatttc tttgattaat taaaaaatct aacactttac tacgctactt
     3841 attttaataa cttaatcttc cactagcttt tcataaaatt ttgttttaaa attaatttta
     3901 aagtgcatat gttatttaaa tacattagat aaaattcgtc attaatctac ttaaagcata
     3961 tttaactaac accactcaac cagaaaattc agaaaactgc attgacacag gaaaaagttt
     4021 attttatact ttttttagga catattgtgg acacgtctta aatcgacaag cagaatttat
     4081 attgtaagat taaccacaat acttattttt taattatatt tttaaacatt agggaggata
     4141 ttatgaatga taacgtagtt aagaagaaaa acttttttga caggacatta aacagaatag
     4201 aaactgttgg taataaacta ccagacccag ttacaatatt tttagggtta tgtgtacttt
     4261 tacttatact atctagtcta gttggttcta tggggatatc agtagttcat cctggtactg
     4321 gagaaactat aaccgctgta aatttattga ctgtagaaca gcttcaaatt cttttaggaa
     4381 acatagtcag caatttccaa gggtttgctc ctttgggtct agttctagta actatgatag
     4441 gtgctggtgt atgtgacaaa acaggactta tgacagcaac aatcaaagca tcagtaagca
     4501 agattcctga aacaagagta actcttgttg taatgactat aggtatgctt gccaatatag
     4561 cttcagatgc aggtacaata ttatttccac cattagctgc acttgtatat cttggtgttg
     4621 gtagacatcc acttataggt ttattttctg gatatgcagc agtctgtcta ggatttgccg
     4681 caaacattat gataagtgtc aatgatatac ttgctgcaag ttttacagtt ccagcagctc
     4741 aaatgattga ttctggattt caagccaatg caacaatgaa cttatttttt atgatagcat
     4801 caacttttgt tctaatactt ttggcaacat gggtaacaga aaaaattgta gctcctagat
     4861 ttggtaaata tgaaggaaat gcagagctag atgttgatgg aagtcttagt gatgtagaaa
     4921 agaaagggct taaaaaagct ggtataagta tattaatata tgtggctatt atagtagctc
     4981 ttagtgtaat cggtaaaaaa ccattcttag ctgacccaga aacaggagat ttactatcaa
     5041 acaatgctcc tcttatgaaa ggtatggtac ctataatagc actagcattt ttcatacctg
     5101 gtgttgtata cggaaaaact ataggtaaaa taaagaagga taaggatgta gtctctataa
     5161 tggctagtgc aatgagtgat atgggtggat atataatact agcatttgct gcttcacaat
     5221 ttttgcaatt attcacaaat agtaatttag gtctgatatt agctgtaaag ggcggagaat
     5281 ttttaaaatc agcaggaata aatggacctt tattgttgat aggatttatt atattatcat
     5341 gttttataaa tttatttata ggtagtgcat ctgcaaagtg ggctattctt gcacctatat
     5401 ttgtaccaat gtttatgatg gttggataca atccagcact tactcagatg gcttatcgta
     5461 taggtgattg tataacaaat ccacttagcc cattatttcc gtacttccct attatactag
     5521 cattcacaag aaaatacgat aaagatgctg gtatgggtac tgtaattgct aatatgatac
     5581 catactctat atcattctta atagtgtgga ctatactact aatattgttt atggtattta
     5641 atattccact aggacctgga atacttcctt catatagcat gtaatttatt taaatctttc
     5701 taaaaggaga aataatttat gttagataaa aatagactta taaataattt tatggatatg
     5761 gttagaattg atagcccatc taatcaagaa ttagaaatgt ctaagtggtt ggtaaactac
     5821 ttaaaagaaa gaaatataga tgccattata gatgatgcag gtgaaaaata tggtggtaat
     5881 acaggtaatg tcattgccta tataaaaggt gaagaaggaa gtagaccact ttgtttatgt
     5941 gcacacatgg accaagttca accttgttta ggtgtaaaac caatactaga tggtaatgta
     6001 gttagaagtg atggaactac tacacttggt gcagatgata aagctggaat agctgctata
     6061 cttgaagctt tagaacatgt tattacagaa aaaataccac atagagatat ttatctttgt
     6121 tttacaatct gtgaggaagc tggtatgcat ggtgtcaaaa actttaatcc tgacaatttg
     6181 ccttgtaaag atatggtgat tttagattct ggtggagcta ttggttcaat agcatacaaa
     6241 gctcctgctc aacaatctat aaaaatctct tttcatggaa agaaagcaca tgctggtata
     6301 gaacctgaaa aagggttaaa tgctatatta gtagcttctc atgcaataag caatatgcac
     6361 ataggcagaa tagattcatt gactacttca aatataggta aaatagaagg tggtggagct
     6421 acaaacatag taactgataa agttacattg actgctgaaa ttaggtctca tatacctgaa
     6481 actttagagt atgagcttaa tcatatggaa aaatgttgta aggatgcttc atctaaattt
     6541 aatactactt atacctttga acatagtatg tcttacccta gttttgaact tagtagagat
     6601 agtcacgttt tcaagttaag tgaagaagca ataagacaag ttggtgttgt tccaaatcca
     6661 atggtaattg gtggtggaag tgacgcaaat atattagcta atcttggata taattgtgct
     6721 atattaagtc taggtatgta tgatgtacac acagttaatg aatatgtaaa tatagacgaa
     6781 ttatatgata ctgccaaaat agtttatcat atgattaaat tataaatatc ataatataac
     6841 tctttaaatt ataaaaataa aaacctatgt atcttcaact ttataagtta tatattaaag
     6901 ttgtttta
//
LOCUS       DAEMHW010000190         6882 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEMHW010000190 DAEMHW010000000
VERSION     DAEMHW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.190"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1194
                     /locus_tag="KPK67_003173"
     CDS             <1..1194
                     /locus_tag="KPK67_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2460097.1"
                     /translation="YLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICA
                     RMDNDHFILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDI
                     KKIDLLIDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVY
                     YQPKYSLDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCK
                     TLNKWINKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFD
                     NLNILIDIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRG
                     KIIISKIVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHL
                     NFKFD"
     gene            1341..2087
                     /locus_tag="KPK67_003174"
     CDS             1341..2087
                     /locus_tag="KPK67_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460098.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            2087..3967
                     /locus_tag="KPK67_003175"
     CDS             2087..3967
                     /locus_tag="KPK67_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460099.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAILLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGAVCLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSREHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            4086..5477
                     /locus_tag="KPK67_003176"
     CDS             4086..5477
                     /locus_tag="KPK67_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2460100.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            5584..6624
                     /locus_tag="KPK67_003177"
     CDS             5584..6624
                     /locus_tag="KPK67_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG2460101.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
BASE COUNT         2716 a          665 c         1159 g         2342 t
ORIGIN      
        1 tacttagtac aattggatgt caataaattt aaatatataa atgagatgtt tggatatgct
       61 gaagggaata agatattaat tcatatatca caggttttaa ataataatac aaataaatat
      121 gaaatttgtg ctaggatgga taatgaccat tttatattgt tgattgcttg taacactgaa
      181 gatgaacttt taaatagatt atctaaaata aataaagaaa tttgtaactt aaatactact
      241 aactcatcta agtataaaat tgttatgtca agtggaatat ataagattac taaaaaggat
      301 gatattaaaa agattgactt gttaattgat agagcaaata ttgcagcaaa aagtaaaaag
      361 gaaaaatatg aacattcata ttcatttttt aatgaagata caagaaatcg attgtacaag
      421 gaaaaacgat tggaagataa tatgaataaa gctttagaaa aaggtgaatt tatagtatat
      481 tatcaaccta agtatagttt agatgatgta aatgaaattg aaggagcaga agccttgata
      541 agatggaata gtccagaatt tgggtttatt tcacctatag attttgtacc tttatttgaa
      601 aagaatggtt ttattgtaaa tattgatatg ttcgtgtttg aagaagtatg caaaacatta
      661 aacaagtgga taaataaagg gtatactcct gtaccaatct ctgtaaatat gtctagagtt
      721 catttatata gggataattt tatagaaaat ataactgatt tgataagtaa atacaatata
      781 tctcctgagt ttattgaact tgaactaaca gaaagtgttg tgtttgataa tttaaatata
      841 ttgattgata taatgaaaaa gattaaagag ataggattcc ttatatcaat ggatgatttt
      901 ggttcgggat attcatcttt aaatctgtta aaagaccttt cttttgatat tttaaaatta
      961 gatagaggat ttttaatcga aacaacagat acaaaacgtg gaaaaattat tatttctaag
     1021 attgtggaaa tggcaaaagc gattgatatt aaagtaatct gtgagggcgt agaaacttat
     1081 gaacaagtag agttcttgaa agagataggt tgtgataagg ttcaaggata cttatttgct
     1141 aaacctatgg tattagatga atttgaaaaa cacttaaact ttaaatttga ttaatcatta
     1201 ataattatta gactataaga taccctaaca acttacttac aaatatgtaa gttaattgtt
     1261 agggtatttt tatggttaaa gtagtatatt cacgatatta tggtattgta gtaaaaataa
     1321 aaaattatgg agaatataaa atgaacatat tgagtattag aaatatatca aaaacatatt
     1381 gcggaaatat tccatttaaa gcattggata aggttagtct aaatatagaa aagggagaat
     1441 ttgtgtctgt tatgggacca tctggaagtg gtaaatctac tcttttaaat ataatatcta
     1501 cagttgatag acagagtgaa ggtgaggttg tattagatgg atatgatgta agtaaattga
     1561 aaggagaaaa acttgctgaa tttagaagga aacaacttgg ttttgtattt caggatttta
     1621 atttgattga cactttaact gttggcgaaa atattatgtt gccacttaca ctagaggggg
     1681 caagtatcaa agatatgaat atacaaacta aaagtatatc taaattttta ggaatagata
     1741 aaattttaga tagaaaaacc tatgaaattt caggaggtca agctcaaagg tgtgctattg
     1801 ctagagctat tataaataaa cctgccatac ttcttgcaga tgagccaact ggaaatcttg
     1861 attcaaaatc tactgatgat gttttgaaat tatttactag gataaacaaa gaacaaaagg
     1921 taacaacact tatggtaact catgaagcat atagtgcaag ttattcagat agagtaattt
     1981 ttataaaaga tggttgtata tatacagaaa taaaaaaatc tgaaagcagt aattctttct
     2041 actctgatat actagctgtt ttgtctcaaa taggaggtgt aagatagtgg attttagaaa
     2101 gtttgcttat aataatatag ttagaaattt cagggcatat ttagcatatt ttttaagttc
     2161 tgttttttct ataatgatat tttttgtttt tgcagtgtct atgttccatc cgattataac
     2221 aaaatctgga atgcagagtg gttctactgc atttatggct cttatgactt cagaattaat
     2281 aatattaggt tttagtctac tttttatatt gtactcttta ggaaatttct taaaatatag
     2341 attgaaagat tttggagtat tgatgataat tggaatgagt aacagacaac tgaaaaaatt
     2401 gatattaatt gaaaatttga taataggatt tttagcagtg atgttaggaa tattacttgg
     2461 tttatcaatt tcaaaattgt ttttattgta tttaagcaaa ctgttttata tgaatttaac
     2521 tgaattttat tttccagtaa aagcaatact attgactatt gtatcattta tgattttgtt
     2581 tttaataaca ggaccaatga gcttaaaatt attgagtaaa aagagtattt tagagctttt
     2641 agtaggtaca aaaaagccta aaaaagagat aaaatcttca aagctatttg gaataattgg
     2701 tgcagtgtgt cttatattag gatatgctat gatactattt ggcaataagt taaaaataaa
     2761 tggaggtatg tatacaacag cactattaat tactttgggg atatttttat ttttttctca
     2821 atttactatt ttgatattaa agtatttaaa aaataaagaa aaattttata agaacaaagt
     2881 aaatatgtta cttataagta atttgatata taaattaaag gataatacac gattgttatt
     2941 tttaataaca atacttttat cggctacttt agtagcattt acaacaactt caacacttgt
     3001 aagttctcaa ggagaagctt ctaaaaataa tttcccaatg gtttactcat atttttcaga
     3061 gcataataat gaaaaagaat atgaagaact acaacaaatt agagaagcaa tcaaaggata
     3121 tgattataag gagttaagtt ttcctgtact taaatataat agggaagtct tactaagtgt
     3181 aaagaattat aacaaattat caaaggaatt gggattagat aaaattagtt caaagttaga
     3241 gaaatatgaa gggattataa ttcctagtag taattcacga gaacatataa attctttaaa
     3301 acaattaaaa aattatagta taaaaaattt aaatatagaa gtaaaaaaat caaaaaataa
     3361 aataattttt cctacaggac tgtttagtaa agttgtagtt ataagtgatg acttatacaa
     3421 tgaagtaaaa ggtgaatttt caaaaattaa ttttcatgga tttgattata aaaactggca
     3481 aagcagtgcg aagataactg aaaatttaaa agaaaaacat tttaatttgg atagagaata
     3541 taacaggtca tattttttaa cattaccaga tatgtattta gtagagttac aacaaagtaa
     3601 aattcttcaa tttatgggtg tttttatagg agtgatatta tttatagcag tttttagttt
     3661 tttatacttc agattatata cagacgagat agaagataat gtaaagtaca aaaaactatc
     3721 aaaaataggt ctatctttta aaaatatgaa taagattgtt agtatagaga taggtacatt
     3781 gttttttata ccgtatttta tagcaattat aaatagcata atttctttac tgtttttaaa
     3841 tcaagtatta aataatagtt ttaagttaga aaatttgggt atattactac tgatatcatc
     3901 tatatattca ctttattttt acttattaaa aaaaatttat actagaaaag tttggttttt
     3961 tgattaaagt ggtatataat agatatataa attaatattt aaaaagtagt taatattagg
     4021 ggagggcatt tgcacctccc ttgtaatatg tggaaaattg aatattagaa gaaggaggag
     4081 acttgatgga aaatcaacaa ttattaggaa cagaacgaat tagtaaatta ctactaaaat
     4141 attctatacc agctataata ggtatgttgg taaatagttt atataatgta gtagatagaa
     4201 tttttattgg aaatatacca ggggttggac cacttgcaat aacaggtctt ggtgtaacaa
     4261 tgccaataat gacaataata cttgcatttg ggatgttgat aggtatagga actactacta
     4321 ccatatcaat aaaacttggg caaggtaaag tagaagaggc aagaaaactt ataggaaatg
     4381 caatgacgtt atctgtaata acaggaatta tcataatgat acttggaata ttatttgcaa
     4441 ataaaatact tacattgttt ggagcaagtg aaaatacttt aatatatgca aaatcatata
     4501 taaatattat attattaggt acagtagtaa atcttctttc cttttcttta aaccactcaa
     4561 taagagctga tggtagtcct aaaatttcag caggaataat gatagtagga tgtttaacta
     4621 atatagtttt agactggata ttaatatttg gattcaattt gggaatacaa ggagctgcaa
     4681 ttgctacagt aacttctcag gctcttacag ctattttaac tatagggtat tatataagtg
     4741 gaaaatcaaa cttacgattc agtaaatcta atctaaaatt agataaaaag ctaataaaag
     4801 cagtgtttgc aataggtatg tcgccatttg ctatgcaact tgcagcaagt ttagttcaag
     4861 taatatcaaa tattgcactt aaaactcatg gtggagattt agctataggg gctatggcta
     4921 ctatttcttc tattgcaatg gtatttttga tgcctatctt tggaataaat caaggagcgc
     4981 aacctattat aggtttcaat tatggagctg aaaaatatga tagggttaaa aaagcatatt
     5041 taggttcatt agtagtagct actataattt tatgtatggg tatggtagta gttatgcttt
     5101 tcccagaagc tattataggt atatttaata aagaccctga acttatgaat atatctgtta
     5161 atggattaag aatatactta cttatgttgc caattgtagg cttatcagtt acaggaacaa
     5221 actttataca atctatagga aaagcaaaaa tggctatgtt attgagcctt ttaagacaag
     5281 taattttatt gataccagca gtattaattc ttcctacatt cttaggctta cagggtgtat
     5341 ggacagctca accagtgtct gattttatag ctacagtaat tacaggtata gttgtattta
     5401 gagaattaaa aagatatact cctaaaactg aaaaattaaa tgaaaatgaa agactaaacg
     5461 agataactac tgagtaaaaa tataaattca gataattgag caagaaattg caaaaaatac
     5521 gattatatga taaaataaaa ccgctgtcaa ggataagaga aattctaatt atggagtgat
     5581 tttatggaat ttacaaagaa aagcaaatta aaaaatatgt atgtaaacat gaatgtagct
     5641 agaatgttgt ctaagataaa tgagtataag ggtagacaac tgctttacaa aaaacaacca
     5701 aaagaaattt tagaaaactt agaaaaaaaa tctttagtag attgttctga atctacatac
     5761 attggaaatc aaagagatag tagtaatttt aatctagaaa agttgataag taatgaagtt
     5821 acacctagaa gtagagagga attgagtatt gttgaataca gagatgtggt aaaaactatt
     5881 aacagtgctt atgagagtat tcccatcagt tcacaaacga ttcttgagct tcatggatac
     5941 ttatataaat tttcatctac tagaggagga agttataagt cagataatga ttttattgaa
     6001 cacaatttaa aacctagcga atttataagt attgattcaa gtgtcaataa agttgaaaaa
     6061 gctgtagaag aaatatgtga agcttataat acattgatag aggaagatga aattgatata
     6121 ttaatattaa tttctgcatt tgtgttagat ttcatattga tacatccatt caaagaaggt
     6181 aatataaaaa tggcgagagt acttatccta ttgttactaa ataaaaatgg gtatgaagtt
     6241 ggaagatata taagtttagg aaagatattt gatgatagtt catatgaata ttatagtaat
     6301 ttaaattatt tgaaagcctc aataggtagt gaaaaagcag atatgaatgc atggattgag
     6361 tattttttag agacgatatt aactgcatat gagaagctgg atgatagttt aaatatatct
     6421 gataagaaaa gacaaacaaa aactagtaga atagagaaaa taataaattc tacccttgga
     6481 tattttacaa aagaagatat aagagattta tgtcctgaca ttccagaacc tactataaat
     6541 agagttttta ataatctaag aaaacaagat aagatagaag ttgttgcaag aggaagaagt
     6601 gcaaaatgga agaaaaaata ttaaaaaatg tttagataac taaaatggta tagtgtttta
     6661 cactatacca tttttaatgt cattagcatg agcaataatt taacaactta agcaatatca
     6721 attttattta ttgattattt ttgaactgaa aaatggcgtt atatggtata cctcagtggt
     6781 aattaatttt ggatgagaag atataatatt acagaatata ttaggagtat atgtagaaaa
     6841 aatataataa aagtgtgagg cttagtaatt aacaaattat gt
//
LOCUS       DAEMHW010000191         6841 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEMHW010000191 DAEMHW010000000
VERSION     DAEMHW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.191"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            614..1264
                     /locus_tag="KPK67_003178"
     CDS             614..1264
                     /locus_tag="KPK67_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG2460102.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            1467..2138
                     /locus_tag="KPK67_003179"
     CDS             1467..2138
                     /locus_tag="KPK67_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2460103.1"
                     /translation="MAKILVVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            2135..3265
                     /locus_tag="KPK67_003180"
     CDS             2135..3265
                     /locus_tag="KPK67_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2460104.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3709..4554
                     /locus_tag="KPK67_003181"
     CDS             3709..4554
                     /locus_tag="KPK67_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460105.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4554..5582
                     /locus_tag="KPK67_003182"
     CDS             4554..5582
                     /locus_tag="KPK67_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460106.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5585..6598
                     /locus_tag="KPK67_003183"
     CDS             5585..6598
                     /locus_tag="KPK67_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460107.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKGFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TRGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
BASE COUNT         2579 a          653 c         1170 g         2439 t
ORIGIN      
        1 ttatatatat taagatttat tattatattg agccttatat taaattaagg actataattt
       61 gaaaattaag atataaaaac tcaatattgt tttgttcaga gataaaaaaa gaatacttta
      121 ggtgattttt attaaaaatt aagagtgata ggattatata ctttataaat gtaaatatga
      181 cgaataaaaa tgataaatat aataatatat atatttacat taggagtttt ctatactata
      241 attatttaaa actaaatata aatgttggaa ttatttagga cttgaaattg tctaaaaata
      301 aatataagtg atgaagaggt gatgtgatga gattagatat ggatagtatg ttatatgtac
      361 taatttatat gacattattg gtatctatca ttggaagtat attttatttt tgtaaagacc
      421 tcttaaatca taaaattaag tatgttttaa taagagacat atttgtaaat agaattacat
      481 attctgaaga acgactgcct aaacagtata tagtttttca gaaatatgat atttggaggt
      541 attgtagttt gttttaattt gaaatgaatc tattataaat tctaaagttt aaaaataaaa
      601 aggagagaat aaaatggatt tcatttcaag tatattgcaa ggattattaa gtatattatt
      661 taattttaca ggtgattttg gaatttctat tgtacttata actttaatag ttaaattagt
      721 acttatgcca ctatcattaa aacaaagatt ttctgtaaaa aaacagcaag aattagctga
      781 aaaaatggaa catataaaag aaaagtataa gaatgatact aaagaattgg aaaagcaaat
      841 acaagtaagt tctgtagaaa gtatgaagag tatgctggga tgtcttatag ttttggttca
      901 aatgccagta gtatatgctt tatatcatgt atgtttagat atgacaaagg aatttacaac
      961 aattataatt ccatggattg ctaatttagg tacatttgat aatttgttta tattaccttg
     1021 tatatataca ttaattatgt tagcttcaaa tttagtgaat tatataccat atttaaaaat
     1081 aaattctcaa gtaaagttta acaaacaaat ggctgtggtg actaccataa caagtcttat
     1141 actaactgtt agaactccag tagccatagg tctgtacttt ataacaagtg caatttattc
     1201 attaattgaa gatatttgtt ttagaatata ttttaatcga aaaaatagaa tagtgacaaa
     1261 ataatataat tattattaga aatattttag cctgctaaca tagaaaaagt tagtaggcta
     1321 tttttatcta tgatatcaat tccattaaga atagtctaaa aaatactatc aaaatgctat
     1381 aaaaaatact ataaaaaaat agttgaaaaa taaataaatt aatataaact aaaataaagc
     1441 ctacaaaaaa gaggtgataa atagaaatgg ctaaaatact agttgtagaa gatgaaaaaa
     1501 ggatgcaaag tattattgta gaatatatgc aaaagggagg atatacttgt ataactgctg
     1561 atgatggagt cgaggcactt acaatattaa agagcaacaa tatagattta atgatattgg
     1621 atattatgat gccgtattta gatggatttt cagtgtgtag agtttcacgt gaaatgacta
     1681 atatacctat tattatattg acagctaaag gtgaagagga ggacaaatta aagggttatg
     1741 aatatggcgc agatgattat ataacaaaac catttagccc aaaagtactt ttagcaaaag
     1801 ttaatgcttt gttacgtaga tacactactg atattcccaa aaattcattg agtttaggaa
     1861 agatttttat tatggtagca tcaagacaag tgtatgtaga agataaattg attgatttaa
     1921 catataaaga atttgaatta ttacgtttat ttatggaaaa ccctaatcaa gttttttcca
     1981 gagagaaact tttaaattgt atatggggat atgattttga aggaaacaca cgaactgtag
     2041 atacacatat aaaaacctta cgcaaaaagc ttggtagtga aggacaccat atagttacat
     2101 taattcgttc aggatataag tttgaggtta aggaatgaat agtttaagaa agattataga
     2161 aaaactgaaa aatgtagata tttcaaattg gattaaacaa aattttggac tatattcagt
     2221 tagaaaaaaa gtgtttttac tttctaaatt atcaggagcg ttaattatat tattttatct
     2281 ggttgttgaa gaattttcga taaactctaa atttgaattt tggatttggt ttataatact
     2341 ggttatattt attatgagta ttgatttctt attgggaaaa tttatttcag aaccaatcac
     2401 atctataaat aaatctgcaa aaagtatgtc ccaacttgat ttttctaatc catgtactgt
     2461 aaatactaat gatgaatttg gagaactttc taggagcctg aatacaatgt ctacaaatct
     2521 acaacaggca ttatcagacc tagaaagtgc aaatattcaa cttgaaaagg atgtaaataa
     2581 ggaaagaatg ctattggaac aaagaaagga gttggtagat acaatttcac atgaaatgaa
     2641 gactccactt ggcattatac gtgcgtatac agaggggctt atggatgaag ttgatgaaga
     2701 aaaaaagaaa aattatatga atgttataat agaagaaact gatagaatga acaatatgat
     2761 tgtatcatta ttagacttat ctgcattgga agcaggagtt tctaaactta acccagagcg
     2821 ttttgatttt atagagtttg ttgaaactgt ggcaggtcgt cttttaattg atataccaga
     2881 tgccaatttt tattttacat atgatttgcc agagtatgaa gtttttgtag tggcagataa
     2941 aatgagaatg gaacaagtag tagagaatct agttattaat gcaaagaagc atgttgtata
     3001 taatggtaat ttagatttat cgattacatg tgaaaatggc ttgcttttat ttgaaattta
     3061 taatgatggt aggtttatag agcaagatga gatttcaaag atttggtcta agttttatcg
     3121 tagtgttcaa tctcagcgta caggaggttc aggtctaggg cttgcaattg tttcacaaat
     3181 acttacaatg caagggttag aatatggagt gaataatcgt gatagaggag tagagtttta
     3241 ttttatgatt ccaattgatg aataatttag tattaattta taaattataa gtatctgatg
     3301 taaaatattt ttatttgtat atgtaagcat ttaaagagaa taaaataaaa aaacttaatg
     3361 aaaaaagctt aatgaaaaag tctaataaaa agtttaataa aaagcttaat gaaaagctta
     3421 ataaaaagtc ttgttttcct gatgaaaaca agatttttta ttaagctttt tattatttca
     3481 cttcgatttc acaagagaga cactctgatt tcaccaaata gaaataaaat aggttaaata
     3541 aattaaaaaa tagaagcgta attaaataag gttacaaggg ggagtactta tgaaatggta
     3601 tacatggttg actattatat tgataatatt gctttctatt ccgcttaaaa ttaaatttat
     3661 aaaatggcgg gatagacgac aaaaaaatga taaaggaggt tatgatagat gataaaagta
     3721 ggagagttaa aattttctta tggaaaagat aaacagatat tgcatggact taattttgaa
     3781 gtaaaagaag gagaaatctt tggattcctt ggacctaatg gttcaggaaa atccacaaca
     3841 cagaaaatct taaatggtgt tttaaaaggt tacaatgggc acgtatccct ttttgggaag
     3901 gaagttaaag catatacaga gtctctatat caaaagattg gtgttttatt tgaatttcct
     3961 tatttatata ccaatttaag tgcaattgat aatttagaat atttttcgtc tttttatcct
     4021 aaaaatcaaa ggagagacat atatgaactc ttagatttgt tagagtttaa gaaagagttt
     4081 ataaataagc ctgtatcatc ctactcaaaa ggtatgaagc aacgtattag catggcaaga
     4141 gcattggtaa gtaatccaaa gttgttattt ttagatgagc ctactagtgg acttgaccca
     4201 tctggagctg tactatttcg taagattatt gaagaagaac gaaaaaaagg aacaactatc
     4261 tttttgacta ctcataatat gctagatgca gatttaatgt gtaacaatgt ggcttttatt
     4321 gcagatggaa agattatggt aatagataaa cccaaaaatt taaaaatgaa aaatagcaat
     4381 aataaagttg aagtggaatt tgtatataat ggaaatcgag atattgaaat tgtagatata
     4441 caagagttag aatctggaat aacttttaaa tatgatgaaa tcttaagtgt acattcaaaa
     4501 gaacctacac tagaagaagt ttttattaaa tacacaggaa ggatgttagt gtaatgtatt
     4561 ctaaattttt taccttagta aaaaaagatt tttggatgat gatttatggt aaattttttg
     4621 tgctgacact tggctctctc atactttatt cgtgttatat taattttggt tatgtaaagt
     4681 ttatggatga agatagttat cacaataatg tatatctata taatccagag aatataagca
     4741 tgagtgcatc tccattaatt aatcaagttt cttctatagg tgaattaaaa gcaaaacttg
     4801 atgaagatac aagtgcagtt tgtattgata tgagttctag taagccgaaa gtaattttac
     4861 atgaaagtat taaaggagtt gacaattata gagctgacta tgcattgtca ttattatcat
     4921 cagaaaaaga ccataatagc aaaatatcag acaacaatgc aataattcaa attaatgata
     4981 aggatacatc tgagataaaa caaagaaaag acatgacttg tgagatttta ttctttgaaa
     5041 ttgtagctat tggattttta gggattgcct ctttattatt taaagaaaag cagatgggag
     5101 taatgcgtat tactggtatt ttacctgtaa aaagaagtct ttttatatta tcaaagatat
     5161 ttgctttttt gatatgtgac ctatgtttta cagttctttt agtagtattt aatattggat
     5221 tttttgaagg aataaaaata cttccacaag tattagttca agctagtatt ttatcaatta
     5281 ttatgtcttt gattggaata ggatgctcaa tgttattgag agattttaag caattttctc
     5341 ttatatatct tgtggttgct gtattcataa ctacacctat atttctatct gcaaatacat
     5401 cttttgaaat agattggatt aaatatcatc ccttctatca tgtttatagt cagcttaaaa
     5461 atgcgttttt catgaatttt tcaaaagaat tagtttattt tgtagtgtgt tctttgacaa
     5521 taatagttct atttttactt gtaaaatata tttttaataa agagttggtt agggagggat
     5581 aatcttgata ggattacgtt accagttaaa gagtataaaa aaagataaaa tgtgtataat
     5641 ttccttcttt ttaccagttg ttatggcgat tttaataaat tttatgggaa caattgatat
     5701 atcttcatta ggagagatac gttttggtat agtgaaggat acacttcctg tacaaacaga
     5761 gcagtggttg aagaagtatg gtatggtaag tgtatatgac acaaaaatgc atttaatacg
     5821 tgaaattaag gatattaaaa cggacacaat tggagttgtt agagatggta aaggaataaa
     5881 aactgtgctt tatggaaatg agattaatgc tataaaaaat gttggaaaga cattgccaga
     5941 gatgtttaga ctaaagggtg atttaaaaga tgttgaagtt acaatattgc ctaaagatag
     6001 tattttaaaa gggtttcaaa atgtttttat tgctatgatt ttaattactg ctatgtttat
     6061 gggatgcacc tttaatgcaa tgaatattat atcggaaaaa gaagatggaa tatcatttat
     6121 aaatgaagtt ttgccacaga gtagaagtca gtatattata caaaaagtat ttttgggtct
     6181 tttatgtggg tgtttatctt caatgataac tgtatttata tgtttggata tttcaatatt
     6241 agatatgata atgatgttgt tactcgttgt attttcagca tttatttcat cacttatagg
     6301 tttatttatt ggaaagttgt cagaaggtct tatggtagga attgtttata ttaaaatagt
     6361 aatgatttta tttataggta ctcctttgtt aatatatctt ctaggggtta atacaagagg
     6421 tatagggaat ttatgctata taatgccttc agttgctaca tttaatggta ttatggagat
     6481 tattaatggt aatgtcatta ttatcaaaga tattgtaatt ttggctgtac attgtgtagt
     6541 atggtttatg atatatatta ttttagataa aaaaagaaag ctatctttta gtagataact
     6601 cagtataatt ataattggga caatttaata ggtgatttaa tatactttgt atagtgtaat
     6661 ttatgtagtg caatttgtat actttaattt atgtagtgta atagttagtt ttattttata
     6721 attattcaat ataatttagt aagaagttca gtacaattag agctacttag tggaaaactt
     6781 aataggtact taaactatat aaatacttta ttttcctcca tttgtactat aaatttaatt
     6841 t
//
LOCUS       DAEMHW010000192         6702 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEMHW010000192 DAEMHW010000000
VERSION     DAEMHW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.192"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1162)
                     /locus_tag="KPK67_003184"
     CDS             complement(<1..1162)
                     /locus_tag="KPK67_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG2460108.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKIAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNIN"
     gene            complement(1310..2101)
                     /locus_tag="KPK67_003185"
     CDS             complement(1310..2101)
                     /locus_tag="KPK67_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2460109.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(2137..2793)
                     /locus_tag="KPK67_003186"
     CDS             complement(2137..2793)
                     /locus_tag="KPK67_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460110.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(2786..3751)
                     /locus_tag="KPK67_003187"
     CDS             complement(2786..3751)
                     /locus_tag="KPK67_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBG2460111.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(3857..3963)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4253..5197)
                     /locus_tag="KPK67_003188"
     CDS             complement(4253..5197)
                     /locus_tag="KPK67_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG2460112.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNESTIKIEYSSK
                     QKNSVHTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            5308..5775
                     /locus_tag="KPK67_003189"
     CDS             5308..5775
                     /locus_tag="KPK67_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460113.1"
                     /translation="MENNTIKADDIINYCLSNLDDVLLMNSWGERAIYYNPNGILKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICILSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(5945..6544)
                     /locus_tag="KPK67_003190"
     CDS             complement(5945..6544)
                     /locus_tag="KPK67_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460114.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         2211 a         1087 c          679 g         2725 t
ORIGIN      
        1 tatttatatt tcttatttct tcatctattt tgatttcatt tgtaatatca tttaaactta
       61 ttaagtaatt gctttcatct ttattattac ttaaacttaa caaataaatt ttatcattta
      121 actctaccag tttatcaatt atacttatat cgttatcaaa tttcttcaat ttttcagcaa
      181 ttttttcatc ttcctttata aaagttaata ttttataata attattcata tcctttgaaa
      241 attcatcttt aaatttatta tttgtgtaac ttatattaaa atcatagtct gttactatcc
      301 atacatttgg tattgtatcc agagcttgtc taagtaattc ttttctctgg tctatgtagt
      361 tttttaatgt tctttgtata atcatatcct tttctattgc taaatttatc tttcctaatt
      421 gctctgactt tttacttaat tttttattga ttgaatcaag ttcataatat ggatacacta
      481 ctctattatt aagactgtat acatatacat aataagtatg gttacataat agtaaatata
      541 ttactcctat taatctatct gaatctacta tattaatctt agaaattgct acaaaacata
      601 taatagttgt aaaaaaaaca aaatatgtta aatcttttat taacttatag tgctcaatgt
      661 tactatcaag tttattcaac aaatatagta aattaaacat agatatacaa atattaatta
      721 atataataat catattccag ttaatatcac taaaaatatt tcttataaag taatctaata
      781 tcacaaaagc actataaaaa attagtattg ggcttattct tagttttttt actttagaaa
      841 tggttaagta tataaaagta gacatattaa ttatgcttaa gttaagcaat acaatttgga
      901 atttttgaat ttttaaattt actactccta aaatacttac aaaaactaat acaagacacc
      961 ctactttcaa tatatttata aattcatctg ttttaaagct acttatataa actatagaat
     1021 atccaaatat ccaaagtaaa attattctat acatatttat agaaactgta tacaaattta
     1081 tatttaaaag tgcaataacc aaaaataacc cattagctag tatatagacc ttattcttac
     1141 ttaaagttcc tttagattcc atattcccca ccacttcttc atttttatat agttttacct
     1201 ataaatatgt taccacaatt cttccaaaaa atttgtcact ttctagttat aaaaaaaaat
     1261 gagtaaatat ttttaatttt actcattttg tagattttgt atattcagtt taaaaagctg
     1321 gaactatact tcctttatat tcatcttgta taaatttttt aacttcttta ctattcatag
     1381 cctctgataa agccttaatc ttatcactat ctttattatt ttctctacaa gctattatat
     1441 ttacatatgg agaatcactt gattctatta ctattgcatc cttagtagga tttaagttag
     1501 ctgttaaagc ataattagaa tttattactg ctccgtcaac atcttttaat actgttggta
     1561 attgctctgc attcatttct tttattacta tatttttagg atttttagtt atatcttttt
     1621 tagttataag ctctccatct tttacttcta tcaatttatt ttttgctaat aatttaagtg
     1681 ctctagctcc attagttgca tcatttggca cagctataac agctccatct tttaattcat
     1741 ctaatgactt aactttttct gaatagaatc ccattggctc tatatgaact ttagatgtat
     1801 aagtaagttt atatcctttc tccttcacag tttcctctaa aaatggaata tgttgaaaga
     1861 agttagcatc caaagaccct tcatctaaag ctgtatttgg aagtacataa tcatcaaata
     1921 tttttacttc taaatcatat cctttttctt ttaacaaagg ttttgcaact tccaatatct
     1981 ttgcatgtgg atttgatgaa gctcctacta gtattttttt atcctctttg tttgaacaac
     2041 ctactgctga tattgctatt gctgacacca atgctactga taataatttc tttaatttca
     2101 tttttactcc ccctctttaa aatgtttata attaatttat tttagtttct tataagccag
     2161 atttcctact ccctggatta tctgaactaa tattataagt gctattacag tatataccat
     2221 tactgctgta tcaaatcttt gatatccata aataagtgct atattaccta atcctccagc
     2281 tcctactgca cctgccatag ctgtataacc aagtatagat attattgata atgtaattcc
     2341 agatactatt gaaggcatag cttcctttat cataacttta aatactattt gtctttttgt
     2401 agctccaaaa gattttgctg cttctatcaa tcctttatca acttcattta atgagctttc
     2461 tataattcta gcgataaacg gtgctgcccc aatagttatt ggcactattg cagcagtttc
     2521 ccctatactt gttccaacga ttaatctagt tattggaatc attgcaacta taagtatcat
     2581 aaatgggaaa cttctaaata tatttactat aaatcctaat gcactattta ttgtgctatt
     2641 tggttttaat ccatctggtt ttgtaactac taatatgatt gctaatataa atcctaatat
     2701 tgttgccact atagttggta ctataaccat atacaaagtt tgtaacaatg catttggaaa
     2761 tagtgttgtt aaaaagtcaa ttaaactatt catttatcat ttcctcccat cttatgccct
     2821 tactagttag atattgtttt acttgctctc cacttttatc tgaaatgttt ataataagtg
     2881 aacctaaaat atcatcttta aattgttcta atttcccaaa tattatagac acatctatat
     2941 ttagttctct tgccatagtc gtaataatag cttcattaga aatatcttta gggaatagta
     3001 tctttatatt tgttcctttt ggcaatatta tactttcttc acctattaat tttctaagtc
     3061 ctttagtatt tcttaagaat atttcttctg tatctcctac ctctaatact tctccatttt
     3121 ccattattgc tactcttcca caaatctgct ttataacttc catttgatga gttacaacta
     3181 ttattgttat tcccaatttt ttatttatat cttcaagcaa tgaaagaatt gactttgtag
     3241 tatttgggtc taatgctgaa gttgcttcat cacaaagaag tacttgtgga tttagtgcta
     3301 aagctctagc aattgcaacc ctttgttttt gtccgccact tagatttctg ggtttatcat
     3361 ttttcttttc agatattcca actacttcta ataactctaa aactcttttt ttaatttctg
     3421 cctttgagta tccaaaacat tctaatggca atgctacatt atcaaataca gtttttcttt
     3481 caagcaatga aaagtgttga aaaatcattc ctaactcttt tcttaaatct ctcatttgct
     3541 tttcattcaa tgactttact tctttatcag aaacaagtac actgccttct tggtattctt
     3601 ctaatccgtt tatacatctc agtagtgttg atttccctgc accactatga cctattattc
     3661 caaaaatttc tccagattct atttcaatac ttacatcttt aagcacttga atctttccat
     3721 aatacttatt aacatttttt atgctaatca taaaatttcc tcccatatat tctgctcagt
     3781 ttttatgttt atcctctctt atgtattaca acaaaataaa agcccgagta taaactcagg
     3841 cttagaaagc tatttatact catctatcaa ctatattagt tgcaggattt agcaccaatg
     3901 tatataaaat atactggttg ccgggtttca aagggccagt ccctccacca ctcttgataa
     3961 gacgttattt aatttaattc tattatcatt tgttgctatg ttatatatga tatattcatt
     4021 aatagtattt gtcaataaat ttatttaaat tttttatttt ttattatcgt taattaacta
     4081 tttttaatta gatttctagc tcctaacact ttatatttta atgtctatat aactatagaa
     4141 catcattata agaaataaat taaataacat caatctatct aaatcatctg taaagtaaat
     4201 taagaaaatt aacattttat ccaaactcta tcctactatt aaataaaata atttaaatac
     4261 aatgatgatt atatagctct agtactttag actttatcaa atgtttaagt tctataggtt
     4321 ctattattgt agcatttttc ccaaaagtta aaaaatattc agccacccag ttaagtgtag
     4381 attttggtat atacatatct atgtatccac ctttagacaa gattttcaat cctcttgcca
     4441 acaaaaattc tgtttcacaa cgttttactc cctcatctgt caaattaatt ttaatatgat
     4501 aatctgtacc tacttccata ttcttaagat aatcatgtat agaagataaa accttatatt
     4561 tcttacttga taagttttct atactcaaaa tcttcacaat tctgtctaca cgaaattcct
     4621 taatagattc tgattttata cagtaagctg gacaatacca aagtccattc atggcataca
     4681 aaccaatagg aacaatggta tgtacactat ttttttgctt tgatgaatat tcaattttaa
     4741 ttgtagattc attcattact atattaaaca attctttcaa taatggtgtt gaacagtgcc
     4801 tatctggtgt ccaaaaaaca aatttacgcc ttatattctc aatattatat tgaatatcat
     4861 ttggtataca atttaaaaat ttttttaaaa cagaaatagt ttcttgctca aatggcaaat
     4921 cattataata ttctagtgac tgatatgcaa agaatataga ttttacctca ctttctgaaa
     4981 atgcaatcgg tggaagtata cgctctttaa gtacatgata tccaccagcc gctccaactt
     5041 ttgaatataa tggaaatcca atactctcca gcacctgtaa gtctcttagt atagtacgtt
     5101 ttgatacaga aaattcttct gctaattctc ctgctgtaaa acttcttttt gcatttattg
     5161 ttatcatcag ttcaattaaa cgctcagctc ttgacatatt tatactccta atttaaatat
     5221 agtgacaata attgtcacta ttatatgata agatattctt acatttaaag caataatact
     5281 agtaaagaaa gaagggactg atactttatg gaaaataata ctattaaggc tgatgatatt
     5341 atcaattatt gtctatcaaa cttagatgat gttttattaa tgaatagttg gggggaacga
     5401 gcaatttact acaatcctaa tggtatttta aaacgaggag tatatgttct taccattaaa
     5461 gaaaaagaca gtaatcatga taaaggttcg ttggttagtc gtccaaatgt ataccgtgtg
     5521 aatataggat taaaaaaaga aacttttatt gaaatgtttg gatatattcc aaagcgtcca
     5581 ggtgtaggtc aaatagttga tatggatttt gattttacaa aattggacac aatcatgcca
     5641 catcctatct actcatggat gggatggata tgcatcctaa gccctactga aaagacattt
     5701 gagaacttta aaactttaat aggagaatct tacaattttg caaaacaaaa atttaaaaag
     5761 agaaaaaaca agtaaaaaca tatgaaaata atccctaaat aaatcataat aaaaatatta
     5821 aggttacaag ataaaaatta atatcttaat aacatgcaaa aaagcgaagc caaaaaatgt
     5881 attatttata aggcttcgct atatctcaaa taatttattt caaatttcaa aactatttat
     5941 tctattattt cttattattt cctgttattc ctaatctatc attcatatct tttatcattt
     6001 ttctaaaacc actacctata tcatttccaa catcttcagt tccatgaact aattttataa
     6061 agtcattttg accttttttc atatcttcat cagatacctt atctaatttt tctattctct
     6121 tatctatatc ttttactaat ctttcccctt cagcgattag gtcatcattc attttcttta
     6181 actctgtatc ttctgtttta ccgtcttttt taagagatgt tacaaatgat tgtattttat
     6241 ctctactatc tttatatgca gcctttacat cacttagata tttttcattt cctggatatt
     6301 ctttatcttt aaactgatta tttatacttt ctggagtatc ataaatagaa tatctattta
     6361 gacctgctat ataatcatta tagcttgttg aataataatt catatatcct tcattattat
     6421 ttacagattc attaggattt tttcctgtat tttcacttgg tttttcatta tttttagtac
     6481 atgcagttat acctatagct aatactagtg ctaatcctac aaataaagtt ctttttaatt
     6541 tcatttttat taccctccta gatgttttat actataaaaa attttaataa ttttctttct
     6601 taatttagta tttctatatt tttatatttt atttactaaa attaaacaag ttataattta
     6661 tttttttgga aatcataatg aataaataat taaataaaaa aa
//
LOCUS       DAEMHW010000193         6696 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEMHW010000193 DAEMHW010000000
VERSION     DAEMHW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.193"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..887
                     /locus_tag="KPK67_003191"
     CDS             <1..887
                     /locus_tag="KPK67_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG2460115.1"
                     /translation="KKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYADLAS
                     KNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDILDEL
                     EFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILPSIT
                     IAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIYDDK
                     NESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAARLIE
                     LIQQYNLQLIDSEIQSEM"
     gene            1330..1821
                     /locus_tag="KPK67_003192"
     CDS             1330..1821
                     /locus_tag="KPK67_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG2460116.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYTGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKVNLKYLIQDITSSILDDNLFISVTAIISYENSF
                     KIE"
     gene            1849..2796
                     /locus_tag="KPK67_003193"
     CDS             1849..2796
                     /locus_tag="KPK67_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460117.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIVKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            3086..3628
                     /locus_tag="KPK67_003194"
     CDS             3086..3628
                     /locus_tag="KPK67_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2460118.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGN
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            3956..5449
                     /locus_tag="KPK67_003195"
     CDS             3956..5449
                     /locus_tag="KPK67_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2460119.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            5688..6452
                     /locus_tag="KPK67_003196"
     CDS             5688..6452
                     /locus_tag="KPK67_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2460120.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHTAILSNGLDFSNVAKKFLEE"
BASE COUNT         2892 a          565 c         1058 g         2181 t
ORIGIN      
        1 ataaaaaatt cattgttata atactgataa tatcaatttt aataggtgta ttagtgaagg
       61 aatattcatc tagagagata aaaaaggatg atataaatgt cagtaagtat ataaaatatg
      121 ctgatttagc aagtaaaaat aatgcacaag ttaactggaa gtatgtagct tccattgttg
      181 ctgtgttaaa taaaaataac ttaaaaaacg ttaaggattc acagatacaa gaagtatcag
      241 atttatttgt taagaatttt tctaaaaata ataaaataaa taagttaagt gatattttgg
      301 atgagttaga atttagtaat agacaaaaaa gattggttga taattatata gacaatctta
      361 aggattatgg tataaaacca gaaagattga agtctgatac aaaatatatg aagtttattg
      421 atgaaattaa aacagaagca atacaaaatt ataaggatta taaaatactt ccatctataa
      481 ctattgcaca agctataata gaatctagct ggggcaagtc aactttggca aaacaatata
      541 acaatctatt tggtataaag gctgacgctt attggaaagg taagtcagtg actttagaaa
      601 cgaaagaaca tttagataca attatagatg ataaatttag aatatatgat gataaaaatg
      661 aatctataaa agaccatgca aaatttttag ccacaaataa aaggtataaa aacaatggtg
      721 tatttgatgc aaaaacgtat atatatcaag ctaaagcatt agaaaaggca ggttatagta
      781 ctgcaaagga tgaaaatggc aatagtatat atgcagctcg tctaatagaa ttaattcaac
      841 agtataatct tcaattaata gacagtgaaa ttcaaagtga aatgtaactt tgattaaatt
      901 tttattgata aggctatgat ttttaaaatc atagccttat tttgacatgt ttttacttgt
      961 aattattaaa tcgatatcat tatctttcaa aatttacttt atactgtaaa ttaaattatt
     1021 taagttaaaa cttggaatta tttataaaag gattatttac tttatctaaa ttctgcactt
     1081 aaagaacatc ttttccctta agactgaccg ttagtctata aaaattataa ttacttataa
     1141 aaaagtgaat agagagatat atatataatt ttcagaaaga cttttacttg taaattgaaa
     1201 ttaatgaagt aaaaaaggat tctttcttaa aagtaaataa caactagata gtgaataata
     1261 aaaaatggta atttgaaata atttttatga atagtattaa tatatagata cagggagatt
     1321 gatgcgatta tgtatgaaaa taataaggta atatacaacc ttgaaagatg tgaaaaacta
     1381 gaaaagactc tttatttatc tcaatttaat acatataaaa attttaaatt agcttctaaa
     1441 aaaactgtaa taaattcatt agagacatat gtttacaata tgaatattaa caatataagt
     1501 cttgttgata ctatagaatg tatatcatta gaagatgaac aacttacagg aaagaagttg
     1561 attttagttg gtgatataga tattgatgca atattagatt atacaggcaa taaaagaaat
     1621 agaaattcta gtaaaaaaaa tacatttaaa ttaaaaatac cctttagtac atttatacaa
     1681 atgcctagaa agatagaaaa taaagataaa gttaatttaa agtatcttat acaagatatt
     1741 acttcatcta ttttagatga taatttattt attagtgtta ctgcaattat aagttatgaa
     1801 aatagtttca agatagagta atctttaaat tatagaaagg tggatgcaat gagaataacg
     1861 aaaactactt ttaatgatgt attttcaaat gatgcaaatg gaagtattat aaaagtagaa
     1921 aaatatgttg ataaaaaatg tgcatctgta tttgagattt tgacctacac tattattgtt
     1981 acaaatacaa gtaggtacaa aactggaaat atatttttca aagattatat ttctaaatat
     2041 atagagttta taaataatac tgtaaaagtt aatggcattg taaaacgtgg attagaccca
     2101 cagcaaggtt tttacatagg gagaatagat gcaagttgta aaaaaataat atcttttaag
     2161 agtgtagttt taccaaattc tgcacatagg attataaaga atagtgctaa tatatactat
     2221 tattataagt gtaatctaga taaattccca acaagaatat caatagaaag taatagagtt
     2281 tatactaatg taaataaaat tgtattcaaa caattgaata taagtaatac tttaaaagct
     2341 cctaaaaatc taagagatat attaaaagta aatgtaaata gtaaaatatt tgatgttaaa
     2401 cctatgaaaa atcttataaa caaaggtagt aatttgaaat tatgtaactt gatagttttt
     2461 gggtgtatag aagttgaaat agactatagt tgtaaaaata ggagtaattt taaaacagta
     2521 aataagtcta atgacattaa aaaagataag tattataaaa atgatataca aaataacaat
     2581 gaaaatataa acgattgtaa aaagcaaata agttttggtg ataataaggt aaaaaaaata
     2641 acaaaaactt ttggattttc ttgtttcatt tgttctccag ttggaattat ttatgaagat
     2701 atgaaaaata taaatataaa aattgaacat acatcaataa atgaattaaa tccagaagaa
     2761 ttatttgtaa atacatcact tttattgtat tattaagtat tttaaagcta aaacttaaac
     2821 ttattaaaat agcatttggt taaaggaaaa tagatattga tatatttatc atatatataa
     2881 atgattaatt aaacaattat aaataataag tatagtataa tatttaaaaa atatagattt
     2941 atgataaaat aagacaaata tgttaaaaaa atgataatca cggagaactt ttaccttctt
     3001 caattaatat attattatta gtaataactt taaaattata ttactattat ataaaaattg
     3061 gcttaaaaga aggtgaaaaa agtaaatgaa aatatgcata acagtaggtc atagtatact
     3121 aaaaggtggt aaaagtacag gtgtaaatgg tatagttgat gaatacaatt ataataaaaa
     3181 attagctcct atgttagctg agatgcttat aagtcaaggt aatacagtag atgtaataat
     3241 ttgtccagaa aggcacttta tgtcagaaag agaagaattt ttctatagag ttccaaaagt
     3301 aaacagtggt aaatacgatt tattagtaga gctacattta aataaagctg atggaacaca
     3361 atgtggtaca gaagtgcttt actatggcaa tgaaggatta gaatatgcta aaagagtatc
     3421 taatagactt ggagaattat ttgaaaatag aggagctaaa aaaagagaaa atttatatat
     3481 attgagaaat acaaatccag ttgcaataca gattgaaagt tttttctgtg ataatgtgaa
     3541 tgattgtaac aaagcaaatg aatcaggtta tgattatata gcacgattga ttacagaagg
     3601 tatattgaat aaagatataa atatgtgaga ttatggtgat ttaggtattg attaatacct
     3661 tttaatatta gatataaata aaaatacaat gtattagtat aatttaaact ttcaaaagat
     3721 agattagttt gattattgac aagagatacc tccttgaaaa tattgattta tcagtatatt
     3781 taaggaggta tttaaatata aaaatttaat taatttgcta ctaaactata ggtttataga
     3841 gtatatattt ttatcttcag tctaataaat aatataaaaa tagtagaacc ttgtattata
     3901 ataatataat gtagaaaaat atatagataa gataaatata ataaggtggt aatatatgaa
     3961 atatacattt gaaaaattta agttaaatga aaagatactt aagtcattga agagtttagg
     4021 atataatata ccttctagag tacaaagaga agttattcca aaattattaa aaggtcaaaa
     4081 cttagtagtt aggtcaaaaa caggaagtgg aaagacagca agttttgcaa tacctttatg
     4141 cgagaatata aatgtagatt acaataatat tcaagctctt atagtggttc caacaagaga
     4201 attagccttg caagtaaaag atgagatatc agatataggg agactaaaga aggtgagatg
     4261 tagtgctata tttggcaaac aatctataaa agaccaaata gctgaattga aacaaagggt
     4321 tcatattgtt gtagctactc caggcagaat acttgaccat ataaatagag gttctattaa
     4381 gttggaaaat gtaaagtatt tagtaataga tgaggcagat aaaatgttta ataaaggttt
     4441 tgttgaacaa atggaaaaaa tattattaaa tttacctaag gagaaaatag taagtctttt
     4501 ttcagctaca atagatgaag agataaaata tatatgtgaa aaatatatgc tagattacag
     4561 tgttataaat atagaagaaa atgagagcga tactaatcaa aaaacaagac aaatagatga
     4621 caaaataatc aaggcaaatg gaagagagaa gtacatcctt ttaaaagaat taatatacag
     4681 cgaaaatcca aagagtgtga taatattttg taataccaaa gagaaagtat ctaaattata
     4741 taacaagatg agtaaagagg gttttttaat aagagagtta catgcagatt tatcacaaga
     4801 aagaagaatt tttgtaatta aggattttaa aaaccaaaaa tttaatatat tagttagtag
     4861 tgatgtagcg tccagaggaa tacatattga tgatatttct ttagtaataa attatgatgt
     4921 gccacaagat aaagaaaact atatacatag aataggtaga actgggagaa aaggaaatag
     4981 tggaaaggca attactatag taactgaaaa ggatgaaaaa tatatagaga atatagaaac
     5041 gtatatagga tataaaataa atgaattaaa agatatagaa aaaagtagaa ttacacatgg
     5101 caaaattttg tttgaagaat attcaaaagg aatcctaaaa aaagtatcaa agagaaaaaa
     5161 agttgataaa aattcttaca aaagtaaaga tataaaacta aatatagaaa gtgaagttgt
     5221 taaattatat ttaaatgctg gtaagaagaa gaaaataaga gttttagata tagttggtgc
     5281 atttagtaat ataaaaggta taactaatga tgatataggt gtaattgaag ttcaagattt
     5341 atgttcatat gtagatatat taaattataa gggagatttg atattaaaaa aatataagga
     5401 gatacctata aagaaaaaaa tggttaaagt gaaaagagat atacgataat ttagttatta
     5461 ctgctctctc tgatgatgaa atcatatatt taagtaaaag tatcaatatt tatgaattat
     5521 tctgttatcg aaatgaagtt gcaaaaaaac aatagaagtt gttaaggaaa agaatattga
     5581 ctttatctac attctaaact atactatttt taaatgttta tgacaatagt cattttaaat
     5641 ataagtaata aatatatacc tatagggata ttaaaggggg gaaatatatg gcatatttta
     5701 actataacga caaacagtgc tactataatg aaattgggga tggtacacca ttactttttt
     5761 tacatggaaa tacagcctct tccaagatgt ttaatgatat aatcgatttt tataaagatg
     5821 aatataaagt aatattaatt gattttgtag gtcatggcaa atctcaaatg gtcgataaat
     5881 tttctgctga cttatggttt gatgaggcta tgcaagtgat atgtttttta gaagcaatga
     5941 actataaaaa agtaaacata attggtagta gtggaggagc tcttgttgca ttaaatgttg
     6001 cacttgagag acctgattta gtgaataagg taattgcaga tagttttgaa ggagaagttc
     6061 ctctagagtc atttgtacaa aatgtgaaaa tagagcgtga agcatctaag caagatgatg
     6121 gtgcaaaagc tttttatatt tataatcaag gagaaaactg ggaaaaagtt gtagataatg
     6181 atacagaagc tatttttgaa cattataaaa ctataggaaa gttttttcat aagcctctag
     6241 aaactatgca accagaggtg cttttaacag gaagtagaga agatgagttt gtatctctaa
     6301 tatctagtga tttttttgaa aatacttttt cattacttct tgaaaagatt aaaaatggta
     6361 aaatgtattt gtttgataaa ggtggtcata ctgcaatttt atcgaatgga ttagattttt
     6421 caaatgtagc aaagaaattt ttagaggaat aatttaacca aaatttacaa taatgaaaac
     6481 tgtaaaatgg attgcaaaac ttacaaaagt gagtgtacat atacttcatt attatgatga
     6541 aatagtattg ttaaaacaaa atattctaat agtcaagtta ttacatttgt atatgcaata
     6601 tgtatagaaa gagatatcat tagattgtaa agttgggatt aaataacaat attaaatttt
     6661 atttattcag atattttagc ttaactatat aaatgt
//
LOCUS       DAEMHW010000194         6622 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEMHW010000194 DAEMHW010000000
VERSION     DAEMHW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.194"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            107..2800
                     /locus_tag="KPK67_003197"
     CDS             107..2800
                     /locus_tag="KPK67_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG2460121.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVEPEVAYVVTDMLESVITEGTGGVAALPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            complement(3074..4009)
                     /locus_tag="KPK67_003198"
     CDS             complement(3074..4009)
                     /locus_tag="KPK67_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBG2460122.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(4053..5225)
                     /locus_tag="KPK67_003199"
     CDS             complement(4053..5225)
                     /locus_tag="KPK67_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG2460123.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            complement(5212..5676)
                     /locus_tag="KPK67_003200"
     CDS             complement(5212..5676)
                     /locus_tag="KPK67_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460124.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            complement(5691..6533)
                     /locus_tag="KPK67_003201"
     CDS             complement(5691..6533)
                     /locus_tag="KPK67_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG2460125.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
BASE COUNT         2269 a         1172 c          970 g         2211 t
ORIGIN      
        1 catatacaat gtacagaata ttatagcaaa ttcatttttt ctattaatta caaaactact
       61 cctatttgtg ttataatatt gcattaagta aggaggttaa aatcttatga ataacgataa
      121 tgataaaaat ggaaataaaa tcaggagaaa aaaagttagc tcatctggta atacaaataa
      181 accaattaat agaacttcag ctaacaagac tcgttcaaca aaaaacaaga aaaaatctaa
      241 aaaaagcgat aaatttaaaa aactaagagt atttgggata gtatttttag ttttactagt
      301 tgttggtaca gcaggtactg ctggtctagt atttgcgtca ctaagagatg taactccagt
      361 tacagaagct gttttagata aacagacgaa ccaaacaact actattaaat acgctaatgg
      421 aaaaacatta tctacagctc caagtgtaaa taaaaagaca cccgttccat tagataagat
      481 ttccccttat cttcaacatg ctgtaattgc aatagaagat gagcgttttt atgagcataa
      541 gggtgtcgat attaaaggtt tatttaggtc agtattaaag acccttacag gtactaagca
      601 aggtggtagt actattccta tgcaggtatc caaaatgctg ttaacaactg aacagcaaac
      661 tatacctcgt aaaataaagg atatttacta tgcacatgag atgagtaaaa cagtaagtaa
      721 agataaaata ctagaaactt atttaaataa cttcttcgtt ggtagaggtc tagcaggtgc
      781 tgaggctggt gcacgtggtt attttgataa gtctgcagct gaccttactc tagcagagtc
      841 agcacttctt gctggttcaa ctaaaaatcc atcaagattt tcagcctata aaacttctaa
      901 gttagaggga aatgaaacta aggaggattt agagaataaa ttattatttt ttgttaacac
      961 aactgatgat gatttagatg accctactca agtggacttt gatatgattg aaaaaataaa
     1021 atcttgggag cttatatcta atgatactta tagacaatta aaagcaggta cattagttgt
     1081 tagaaaggct gtaagtaatc ctgaagctaa aaaaagacaa gaaattgttt taaagaaaat
     1141 gttagaattg aaatatataa aacaaagtga atatgatgaa gcaatcaaag ctaaaataga
     1201 aataaaattg cctcaatcat cagataaagt ttcttcatct gtagaagacc ttatcgaaag
     1261 tgaagttata aatgctttaa tggaacaagg acatacaaat gatgaagctc aaaacttatt
     1321 ctacaatggt ggtctaattg taaacacaac tatagaccct aaaatgcaag atgctttaga
     1381 agaagaattt gatagaaata gcaatttccc aggacatatg gttggtcctg atggagttag
     1441 ccaaccgcaa gctgcaatgg taatcttaga ttataaaaat ggtgaaataa gagcactagc
     1501 tggaggaaga aatataagtg gtagaaaaac tttaaatcgt gctactaacc ctcatcagcc
     1561 aggttcttct ataaaacctt tagcaatata tacgccagct attgatactc taaaaataac
     1621 acaagcaact gctctaagtg attctcgagg aggatataag tttgaagaaa ataataaatg
     1681 gaatccaaga actactactg ctggtcatgg ttctatgagt ttacgtaaag cacttgctaa
     1741 atcttcaaac acgatagctg tcaaaactgc tgaaatgctt ggagactctt atgatgaatg
     1801 tgtagatata atgatggatt acttaaagaa ctttggtata acaactttaa agaataatca
     1861 ctcaggttca tctgaagcaa gtgatagaaa attcccatca ttgacactag gtggtatggc
     1921 gaatggtatt actccacttc aaatggcggc tgcttatggt acattggcaa atcacggtat
     1981 ttatgtagaa ccttctattt ttactactat aactaccttt gatggtcaat tattagttaa
     2041 aaatgctcct gaggaacata aagttgtaga acccgaagta gcttatgtcg ttacagatat
     2101 gttagaaagt gtaattactg aaggtactgg tggtgttgct gcacttccaa agggaatgcc
     2161 tgtagcaggt aaaacaggta ctacaaatag tgcatatgat gcttggtttg tcggttacac
     2221 tccttattat gttggtgcaa cttatattgg agatgatgct ggtagaaaag atgattctgg
     2281 taacactata aaacgtagag aagtaccaca tggaagtact agtactgcaa aattatggga
     2341 aaaaattatg gaaaaaatac atgctaattt aacggttact gaatttgaag ttcctaaaaa
     2401 tgtatatttt acaaaaatta atttagagga tggaggaaag caatcttctg gctctaaagc
     2461 agcatttatt gaaggtactg ctcctactaa agtaagttct caaccatcat ctgaagatac
     2521 aaaaaaacca gacaataacc aaccagaaga aaataataat aacaataata acacaggtga
     2581 taatggtggc ggttctacac ctcctgataa tggcggaaac aatggtggtg gctctactac
     2641 tcctcctgat aatggcggaa ataatggtgg tggctctact actcctcctg ataatggcgg
     2701 aaacaatggt ggtggctcta ctactcctcc tgataacggt ggaaataatg gtggtggctc
     2761 tactactcct cctgatggtg gtggaactcc agcaacttaa taaaaaatag aatttaaata
     2821 aaaatataag gagttgtctc aaggtgatta aaaaaatcac tttgaaacgc tccttttttt
     2881 ataattacaa tatattattt gtattcataa aaaagagaat cccaataacc agaatcctcc
     2941 tttttatata aacaaataaa tattttattt ttaaagtttt taactaagtg ctttattctt
     3001 acttttattc ctaattgttt atatattctt aaatagttat atcacaaatt atataaactt
     3061 aatatttttc taattactgt ataccaaatg tttgaaatct atttaatttg ccatatctct
     3121 taataagcat ttcccactct tctttatcat agaaatcaca cttatttcta ccaaatagct
     3181 tggcaacttc aattgcaaat ctcccagctg aatctaaatc actaaaatta gttgcacctg
     3241 tagcacatcc agcaacaggc atctctgttg taattgctac tcctactatt ggtgcatctg
     3301 tagctactgc tggttgtaaa atactattta agtgatacag attatttcca tatggtgtta
     3361 tatcttgaat agacaaaggg aatacaaatg gagatttccc tgttacagtc tgcataatat
     3421 ctaaaaggtt atcactagtt tttaatatgt agccttcttt aacagttggc gatattgcaa
     3481 atcctctagt attgattact ctatttcctt tagtagtatc tatagaaagt attgcatcac
     3541 aactactatc aacttcattt tcattcatag tcatcatatc aattggagaa tccataaaag
     3601 gagtaggata gtgttcttta gttggagcat ctgggcaaat atgagttgaa attacaacat
     3661 caccatctaa tatgtcccct tttcgttgca tatccaaaag tttagcagcc actgcaattg
     3721 ctaccaatgc tccatcacca tctgagacaa aacctataac ttctggtctt gctccaatcc
     3781 ctcctagtct acctaaaata ccaagagtag gagcatctcc accttttaat tttccatttt
     3841 ttccaggaat tgtaagcttt actaaatctg ttgaaccttt acttgaagac aattctttta
     3901 cagttacatc tacttcacca taaccttgta agtaagtttt tacttcttcc ccattagcat
     3961 ttgctttatc taaaatgtca taaacctcta ttacttgttt taatatcatg tctaatccga
     4021 tttcctttct taatatattt taaaattata tattatttac tatacaaaat atctactcca
     4081 cacattgcta gcaccttaat tgcttttata agtgctactt tgccttcttc accaccagct
     4141 gcaacagcaa attcatctgt atgagttgca gtattttctt ttaattttat atatgcttgt
     4201 attgcaggta tctcatgagt taaatttcct gtatcagttg aacctatacc ttgacttaaa
     4261 tctctttcaa catagtcttc acccaaaact tcaaaatttt gtctaactat attcactaat
     4321 tcagaatcat tcttaatctc tttgtatatt tcatctaatt gaactatttc tacattagtt
     4381 ccagtagaaa ttgcagctcc tttagcacaa tttttaagca tttcacaaat ctcatttaaa
     4441 tattctatgc ttaaggctct tacattaaat atagctgtag atttttcagg aattatatta
     4501 tgtatgctac ctccatcagt tataattcca tgtactcttg cataatcttt aagatgtaat
     4561 ctcatgctat ttacactgtt aaatagtgct attactccac ttaaggcact tttcccttcc
     4621 caaggaaaag cagcagcatg tgatgcctta ccagtaaaat caaattttaa attaacttga
     4681 gcaaatgata tatcatcagg cattgaagca tcacttggat gtaaaattaa tgcagcatct
     4741 acattattaa atacaccttc tttaatcata gttatttttc cacctactct ttcttctgca
     4801 ggtgttccaa aaacctttac agtcccttct atattatgtt ttttcatgat ttcttttaat
     4861 atgatacctg caccaacaga acttgtccct atgatattgt gcccacatgc atgtcccatt
     4921 cctggaagtg catcatattc agctaaaaaa gcaactgttt tcccattagt tccatttgaa
     4981 tacacagctt caaaagatgt ttctaatcct cctacacctt tagttacatt aaattcattt
     5041 tcttctaaca aatcacataa tgctttttgt gacttatact cctcaaatgc taattctgga
     5101 tttgaaaata aatagtcaga aactttttca aatgatgtct ttttctcttc agataataac
     5161 tttatttcct ctttaatttt tgcttttaat tcattcatat ttactacctc cttaaagcat
     5221 tccatttaat cttaaaactg tttgagatat aaaagcagaa ccaaagtaag tagatgtcat
     5281 aactataaat gatataacta ctaacttcca actcaaagtc tttaatatat ctaattggtt
     5341 acctacagag attcctgcaa aagctaataa tggtgttact gtagacataa aattgatttt
     5401 ccctacattt gttgctatga tatcccccac tggagatatt ggaagtgata aaacgaatgc
     5461 aactaatgta gcccatgcaa aacctggtaa attaggtttt ttaacaaaat tctttattat
     5521 aatagcaatt atcaccaaca aaaatccaga tacacttcca atcaacaaat cttgagcctg
     5581 agtcttaagt gctatcattt gtccaaagca aataataata tatataaaca atactatttt
     5641 tatctgctcc ataatagtct taaaagtttt ttccatatat atatcctcct ttacaattta
     5701 ttacttgttc ttcttgtttt tcttcctcta aatctttcaa taactggagc tactttgcta
     5761 taatagtagt tggctactgg taaagataag aataattcta tatatattcc agtaacagct
     5821 gtaatcaatc cactagtagc tgaatatgct aaaatctgct ctgacatttg tggaaccgta
     5881 ttcattaatg atgctgttgc agctcccatc attgaaccac tacccattcc tgatgccatt
     5941 gctaaagcat atggatgaaa tcctaaaagt acacccactg gtgctaaaaa tgaaaataat
     6001 aatgttccaa atatactacc acttatgtaa gttcctagtg ttcctatacc ttctggtgag
     6061 tttaatccat acttatctcc aataattcct agtgctgtgt cacgacataa tgaaaaagta
     6121 ccacctaatg cacttcttcc aagacccaat gctactccaa caggtaatgc tatcaacatt
     6181 gtacccatat gaccaaattc ttgtaataaa agtgctggtc ctgcttgaag taatatacca
     6241 actttgggcc ctgcattaat tccaaacatt atccctaatg gatatagtgt caaagataat
     6301 aaatatggag atacatttat ttcattatcc cctataaact tttttaaggc tggtataact
     6361 ctccctaata aatgaggaga aacagccatt cctatgaata tagcccatag tatactaagt
     6421 aaagttacat taattcccat aactttaaat gaaataggtc caattaattc tgccaatatt
     6481 gtgattataa aggctgctat tatggcattt ctaatccctg ctttttcttt catttctata
     6541 cctccttgta atttttataa ttttcttata atataattgt tagtgatttt attatataac
     6601 ctatcattga taatgtataa ta
//
LOCUS       DAEMHW010000195         6596 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEMHW010000195 DAEMHW010000000
VERSION     DAEMHW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.195"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            208..1179
                     /gene="asrC"
                     /locus_tag="KPK67_003202"
     CDS             208..1179
                     /gene="asrC"
                     /locus_tag="KPK67_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBG2460126.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            1236..2012
                     /locus_tag="KPK67_003203"
     CDS             1236..2012
                     /locus_tag="KPK67_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG2460127.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     NTVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            2283..4301
                     /locus_tag="KPK67_003204"
     CDS             2283..4301
                     /locus_tag="KPK67_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG2460128.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            4516..5373
                     /locus_tag="KPK67_003205"
     CDS             4516..5373
                     /locus_tag="KPK67_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG2460129.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKVDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            5470..6339
                     /locus_tag="KPK67_003206"
     CDS             5470..6339
                     /locus_tag="KPK67_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG2460130.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT         2694 a          608 c         1165 g         2129 t
ORIGIN      
        1 tcacgataga gatataaatg caagtataaa tatattaaaa gaaggattaa gactaataac
       61 gattcaaaat aaataactta tgtaagaacc gtaggaacta cggggatggc ctgtggagaa
      121 ttagtaagac atattttata tgcaaaattc tgtgaagcag gaatcctgtg actttagtca
      181 tgggaggttc agtagggggc gaagtttgtg ataagagatt taaataccaa aaaagtaatg
      241 aaaaatgctt atagaattac aaaaacaaaa tatgagactt ctttaagagt tagagtacct
      301 ggtggtctta ttgaccctga gagtttagct gtagtttcta aaattgcaag tgagtacggc
      361 aatggacagg tgcacataac tacaagacag gggtttgaaa tattaggaat aaatatggaa
      421 gatatggaag aaataaataa aattatacaa cctgttattg aaaaaatgaa tataaatcaa
      481 agtgagaaaa acgcaggtta tccagcagca ggtacaagaa atatagcagc ttgtatagga
      541 aataaagtct gtccaaaggc tcaatataat acaactgaat ttgctaaaag aatagaaaaa
      601 gctatatttc caaatgattt acattttaaa gtggctttaa ctggttgtcc aaatgattgt
      661 ataaaagcta gaatgaatga ctttggaatt attggtatgg ctttaccaat atatgaaaag
      721 gatagatgtg taaattgtgg agcatgtgta aaaaaatgtt ctaagatatc tgttggagca
      781 ttaaaaacag aaaataataa ggtggtaaga gataaagata agtgtatagg ctgtggagag
      841 tgtgtgctaa attgcccaac aaatgcatgg actagagatg agaaaaaata ctatagatta
      901 gctataatgg gtagaacagg aaagaaaaac cctagactag cagaagattt tcttttatgg
      961 gtagatgagg acagcataat aaagataata cttaatactt atagatatgt agaaaagtat
     1021 atagataaag atgcaccagg aggaaaagaa catataggat atataattga tagaacagga
     1081 tttatggaat ttaaaaaatg ggcacttgaa ggtgtagagc ttccagaaat aactaaaatg
     1141 tacgaaaaca tatactggag tggaattaaa tatctataaa ttgagtaaaa catatatttg
     1201 aataaattaa aatttaatag aaggaagagg gaattatgca taaagaaact ttggataaat
     1261 taactaatgc agctataaat aagataaatt tattaaacac aagtaaagta aaatatttgg
     1321 tatcttctgc gtttgcagga ctttatgtag gtataggtat acttttgata tttactattg
     1381 gaggactttt gactgatgca ggttcaccta tgactaagat agtaatgggg ttatcatttg
     1441 caatagcact aagtttggtt ataatgacgg gaacagagtt atttactgga aacaatatgg
     1501 ttatgtctgc tggtatgtta aataaaggtg taagtataaa agatacttct aaaatatggg
     1561 catacagttg ggtaggtaat ttaatcggag ctttggtttt aggcattata tttgtaggaa
     1621 ctggtctagt agacaaagga cctgtagctg aattttttgc aaatacagca gctagtaaag
     1681 catccatgcc atttacagct cttttcttta gagggatttt atgtaatatt ctagtatgtg
     1741 tatcagtgtt gtgttcgttt agaactaata gtgatactgc aaaaataatt atgatattct
     1801 tatgtttatt tgcttttata acatcaggct ttgagcatag tgtagcaaat atgacaattt
     1861 atagtgtttc attattttca ccaacaataa atacagttac tattggagga gctatttata
     1921 atttagtagc tgtaacactt ggaaatattg ttggaggagc tttatttatg ggtcttggaa
     1981 cttatatatt aggtaaagaa aagcttaatt aaatttattg tataaattat aactctcttg
     2041 ataaatgcat ttttagtatt attgagagag ttatttttat atttttgaaa aaatgtttga
     2101 aacaaaaaaa atatgggagc atacgaaaat atatcggtta tatatataaa aaatacgttt
     2161 atatatgaat tatatagttc taaatataaa aaaagttaaa gtcctaccgt aaaaaagcgc
     2221 attttgaata agataacaaa tatgataaaa ttggtcttgt tgaaattttt aggggggata
     2281 tgatgacaaa gaaactagta atatcaaaaa caaagttgtt tatttttata atagcattta
     2341 tttctatatt tatatattta tttggaagta aaaatacact tataggtgta ggtatagtaa
     2401 cagctatgct aacactcttg gaaagagatt taactatttc tccaattaaa aatttactga
     2461 agtatttagc aataaatata atcttaggaa tactatcatt ttttgcagta caaaatatgt
     2521 atttaggagt tttattaaat tttatagcat tatttataat agggtatgta tttagttatg
     2581 acttaaaaag agcagtatat gtaccttttg gtctaatgta tatatttatg gtaagtatac
     2641 ctgtaggaaa aagtgaattt cctatgagat tgtcggcttt agctgttgga gcagttataa
     2701 taatgattgc tcaatttgtt atgaatagaa ataggatgaa aaatgtcgga gataaagaac
     2761 tgatatctat atgtgatgaa ttattagaaa aaattagtct tttaaaaaat actattaatg
     2821 atgatagttc tataattaaa tctagtatga gaaaaataga tagctgtaat tatagaataa
     2881 attctatatc taaaaatttg aaaatggtta tttttgataa tagaaaagat gacttttata
     2941 tcagtattag aggtatagac ataatgaata ttttattttc attagaaaga ataagtttaa
     3001 tcctagaaag atacaaaaag gatagtaaag aatttgaaga tgaagatata aaaaatattt
     3061 tagaagaaag tagcataaat agtaaatcag atgttttagt tgtagcaacg aaagaaataa
     3121 actatattaa gatgtgttta gaaaataaag ataccataac tgataaagag atattaggat
     3181 ttagagatta tgttattgct caggatacta aaaatataaa tttaaaagaa atttatagtg
     3241 ttttagaaaa tttatatgag tttttagtag attataaaaa agtaaaatca agagacgaaa
     3301 agaaagctga aagaaaatca aagatacctc atgaatttaa acgactatca atatataaga
     3361 aaaactttaa tttaaattct ataagatttt cttatgcagt aaagattgct ttagctacag
     3421 ctgtagcggg ttttattatg gattatttcc atttaagaga tggaagatgg ataatgttaa
     3481 ctgtcttttc attaacacaa ccttacgcag aaaattgtat acaaaggtct agaaaaagaa
     3541 tagaaggtac atttatagga gctgtaatat ttattgtatt attttcaata ataaaagata
     3601 gtacattaag gtcgttaata gttcttttag cagggtatat aaactcttat gtagttgatt
     3661 atagaaaatt aatggtatgt gtaactgttt cagctcttgg ttcagcagta gttatgggag
     3721 acccaaatgt tttaaccata agcaggattt catatgtagc attaggagca attattgcat
     3781 taatagtaaa taagtttata ttaccatatg atgctaaaac aggctatcaa catgttatag
     3841 aaatgtataa aggaatagtt aaaaatataa ttgatgaagt caataagtct atagaaaatg
     3901 tagctgatgt ttactatata aaaaatttat tattgatacc atcacttatt gaagatagac
     3961 ttatgttgat aaatgctata tataaagata aacatcaaga ggatttttta gaaaatcaga
     4021 aattactaat aagtaatatg tataatctat atataaatgt taataaaaat aaaataaaag
     4081 atgaagatgt tgaaaaaata ttgagagata ctaattatat ttcaaattac aatgtagata
     4141 agtatgatga aggaagaagt gtaattttag agagtattgt aaatactaaa agtctaggtg
     4201 acaagataat atgtctaaac ttactgcaaa ctctaaatgg tgtcaaagaa atgtatagaa
     4261 taagtaatat aactaaagtt tcaataaaag aagcagctta gatattaaat tatagaatgg
     4321 ccatatattt aaaataaagt atatggctat ttgttttata aaatactaaa agaaaataat
     4381 atataaaaaa caaaaaacat atatttatga attttatatg aaaatagtta aaatattgat
     4441 ttttttcatt ggttgaaaaa acaatttact taggataaac tataatagta ttcaaatatt
     4501 atgggggtta gatttatgag taaagatgta aaggtaaagc gtaataagaa atatgataca
     4561 tttagtataa ctatggaact tgagagacct ttactttatg atgaggttga gaacaaagat
     4621 gataaaaata ataatagaaa atttaacata aagaataaga tatttaaggg aataggtata
     4681 ctatttgtat tgattttgct tggctttttc atatggataa ataaaacata tgaacctcaa
     4741 aagctggcaa aagaagctct agtcagcaat agtaaagttg aagtaactgt aaatgaaaac
     4801 atttcattta caccaaaagg aagaaaagtt tctaaaggtt taattttgta tccaggagct
     4861 aaagtagaaa tagaatctta tgctccactt gcaagaaaaa tagcagaaag tggatatgag
     4921 gttgtaatcg taaagatgcc acttaatcta gctatacttg gaactaataa ggcacagaaa
     4981 gttatggatt cttacaacaa catagaacat tgggtaatag gaggtcattc attaggagga
     5041 gttgcagcat caaattttgc acgtgataat aaattaattg aaggcgttgt atttttagct
     5101 tcttatccaa tgggtgatga gttgaaagaa ttaggaaaaa aagttatatc tatatggggg
     5161 agcaaagatg gagtagtcaa ttttaaaagt cttgtagaat caaaacaaaa acttcctgat
     5221 gatacaactt atgttgagat tgaaggagga aatcatgcac aatttggaga ctatggaaaa
     5281 caaaaggtag ataatgatgc tattataagt caagaaaaac aacttaatat tactgccaat
     5341 agtatcatta aatttttaaa gaatatatct taaactaaaa tttaaaaaat ataattttat
     5401 aagttattat ttaaagtaaa tattaataat ataatagaat gaatatttta ttacaagggg
     5461 gattttaata tggaatacga tatgccactt tacagaccac caagtgaggc ttatagtctt
     5521 ataatacaag tcacactagg atgttctcat aacaaatgta cgttttgtag tatgtacaaa
     5581 tctaaaaaat ttaaaataaa gccactagaa gtaataaaaa atgaaattga tatatttaga
     5641 agatattata agcatgtaga aagaatattc ttagcagatg gagatgcatt gataatacca
     5701 atggagaaat taagagatat tattttatat ataaaagaag tttttccaga gtgtgaaaga
     5761 attactatgt acggttctcc taaatctata gagaaaaaat ctgatgatga acttaaagaa
     5821 ttaagaaaac ttggtgttaa aatgatttat ctagggcttg aaagtggaag caatgaagta
     5881 ttagaagata ttaagaaagg ttttagtagt gaacaattaa taaaagttgg tagaaaagtt
     5941 aaaaaaagtg ggattaaatt atcagccact gtaatcgcag gtcttggagg aactaaaaaa
     6001 acacatcagc atgcagttga tacaggaaaa atgttaggag caatttcacc agattatgtg
     6061 ggtgtactta gtttaatggt agaacctaat actgaacttt acgatttact taaggcagga
     6121 gagtttacaa tactagagga taaggctgtg ttacaagaaa ttaaagagat gataaagaat
     6181 atagatgcta atgaaaaaat tgtatttaga agtaatcatg catcaaatta tgctaattta
     6241 aaaggtgtat tacctgatga taaacaacgt ctaattgatg aaattgatta ctatctaagt
     6301 aaccaaaagc taaaaagaga agaatataga agactatagt ttttgatata tcaatctata
     6361 ttgataggta aatactaaga ttatttagat acaaaaaatc atgctacatt aatagttgct
     6421 tactattgat gtagcatgat ttttttatta aaaaacaaaa tggtatattt atatataata
     6481 tatcaataat aatgaaaatg tgaactagtt taatagaatt ttacctatta taataaaaac
     6541 aggaatttaa acaataaaaa gtaaagtaac tttttattaa gtaactttac ttttta
//
LOCUS       DAEMHW010000196         6543 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEMHW010000196 DAEMHW010000000
VERSION     DAEMHW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.196"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            347..1621
                     /locus_tag="KPK67_003207"
     CDS             347..1621
                     /locus_tag="KPK67_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG2460131.1"
                     /translation="MQFIDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGIHF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMEKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKDAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1829..2146)
                     /gene="trxA"
                     /locus_tag="KPK67_003208"
     CDS             complement(1829..2146)
                     /gene="trxA"
                     /locus_tag="KPK67_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG2460132.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2413..3165
                     /locus_tag="KPK67_003209"
     CDS             2413..3165
                     /locus_tag="KPK67_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBG2460133.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLRILNKN
                     KSVEYVLNIKGEKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3152..4033
                     /locus_tag="KPK67_003210"
     CDS             3152..4033
                     /locus_tag="KPK67_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG2460134.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGMHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTGFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4259..5614)
                     /locus_tag="KPK67_003211"
     CDS             complement(4259..5614)
                     /locus_tag="KPK67_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG2460135.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2516 a          943 c          826 g         2258 t
ORIGIN      
        1 ttctttacta tttttacaac tttattagtc atattcataa ttatttctaa ttagtgttta
       61 acattttatg taaaatttat aaaaaaatgt atataaatta aaataagtga ctatactaaa
      121 aatatgatac ggataaacaa taagttcaac ctatctcccc caaaatcaag tgagctacag
      181 tttcccccaa ctgtagctca ttttcgtgta atttaataaa catctcaaca agaattaatt
      241 aacaaaaccc cttataaaaa cataacatat attaaacaag ctcttgatta aaacttgagc
      301 ttttttaata aaaaacagaa tagtttatta gataaggggt ttttttatgc aattcataga
      361 tgtatctaaa tacattgaag gtgcaaacct taaagttaaa actaaaaatg gtgaaactgt
      421 taaaagaata tattttaaca actctgccac tccattagtt ttaaaaaacg tagtggataa
      481 cttaaactgt gagattcctt ggcttactta tattaatgct ccaggaataa tctcagaaaa
      541 aaatacttta aaatatgaaa atgtacgctc tactatcctc aaacttattg atggagacga
      601 agaaaaggat tctgttattt atgtcaaaaa tgctacagaa ggaatacact tcttagcaaa
      661 attttttaaa gatgaaaatc cagataaatt tgttattacc acagcaatgg agcatatggc
      721 taattacctg ccttttaaag ttaattttcc tacaaaagtt gttggaatta cagaaaaagg
      781 agaacttgat ttatgtcaat tagagaatat acttaaaaat tatgctggta atgtatctct
      841 cgttacagtt acaggagcta gtaatgtcac aggtataacc actcctatat atgaaatagc
      901 aagaatagct cataagtatg gtgcaaagat attggtggat attgtgcaag taattcagca
      961 taaacctttt tcaatgatgc cacatgaatg tgatgagcat atagattttg ttgtttttag
     1021 tgctcataaa tgttattctc cactaaatgg aggagctctg gtaggaccta aaaaattctt
     1081 tgaaaaattt aatcctgtac tatatggctc aggttctacc aaatttgtaa atgataacaa
     1141 aatactatat gcaaacgtac ctcaacgttt tgaagcagga tacccagagt attttggaac
     1201 tcttgcaatg gaaaaagcac tgaacacttt aaataaaatt ggtctaaaca gaatttcaaa
     1261 atacgaaaga gaattatttt tatatgctaa agaagaaatg agtaaaatac ctaatataat
     1321 aatatatgga gataaatcaa ataatgtaat tatcccaaat atatctttta atataagtaa
     1381 tatgtattac aaagatgtag caaaatattt agttgataac tttggaatag aaactggagc
     1441 tggagtagta ggtgcagata tttatgtaca aaaattatta ggtataagtc caaaagacgc
     1501 atacgtcaga tttatgaggg aaaatcctgt aggtcttgtt aggataagct taggaatgta
     1561 caatactttt gatgaggtgg atagctttat acatgctatt aaaactttgg ctttcaaata
     1621 agatttttct attctttttt atcaataaat agagagtagt tttgttttca caaaaaaaac
     1681 aaattatatc aacagtcaat ttcattagca attagacact catttgtaac tatacaaact
     1741 attttacact atacaaacaa aaaactcatt acaatgagtt tttttgttta tatatgagat
     1801 ttattttaat tacttcaatt tactttaatt acatatatga ttcaattttg ttttttaaat
     1861 tctccttagg cataaaacct attaatctat ctacaggttt tccatcctta aatattagca
     1921 tagttggtac tgtagatact tcaaatttct gagctaattc tatactttgg tctatgtcca
     1981 ccttcaagaa gtttgctttt tcaaccatct catttcctaa agcttcatat attggtgata
     2041 gcatcttaca aggtccacac caagtcgcaa agaaatctac aactactaaa ccatctttat
     2101 tttctacttc atttataaat tcattattat ttattatttt taccataata tacatctcct
     2161 ttattatctt cttgatatta atatactacg ccctatataa cagttcaatt aggcactttt
     2221 ttgtaaccgc ctatttttaa tagatttaat attatgataa atagatttat aagattaaat
     2281 atttatgaga ctaaatattt ctaatatatt ttattttttt cttaatatag ttgacatttt
     2341 ttcccttaaa ttataaaatc aaattaataa ctacataggt agttactaat cttttatcaa
     2401 aggagtttct atatggataa tataattaat gaattacaaa atataggatt tactaaatac
     2461 gaatctcaaa tatacatatc attattaaag gaaagccctt taacaggata tgaaataagt
     2521 aaattatcag gtgtaccaca atcaaaagtc tatgaaaata taacaaagct attaaacaat
     2581 aatataatta tcaatgttgg aactgaccct attaagtaca taccaataaa ccctaatgaa
     2641 ttattaaaaa ataagaaaaa agaatttgac aattctatgg ataatctaaa gaatagctta
     2701 cggatattaa ataaaaataa aagtgtagaa tacgttttaa acataaaagg agaaaaaaat
     2761 atattaaaaa aagcaataga aatgataaaa aatgcaaaac aagaaattat aatttcttgc
     2821 ccttatgaag aaattcttga attaaaaaaa gaactcctaa ttgcttacaa aaaaaatatt
     2881 aatattgaaa tactactttt agaaaacaaa agtctgaaag gtcttgataa tattcatatt
     2941 catggaggta ataaaactac acttgaaaat aaaatgcttt acaaaggaat tattttgatt
     3001 gtagataatg atgaaatatt gacaggaaac ttatcagaag gcagtgaggc catttcaatt
     3061 tggacaaaaa actctaacat tttatatatt ggagttcaat acataaaaca tgaaatttat
     3121 atttcagaac aaataaaagg aggttataaa aatgaacaaa attaagatta aacaagttga
     3181 tgcttttaca actatacctt ttggtggaaa tcctgctggt gtcgttactg atgcctctaa
     3241 catttctgat gaaattaagc agaatgtggc taaagaaatg aatttatcag aaactgcatt
     3301 tgtttcagag tcaaatattg cagattttaa ggtacaattt ttcacaccag gtttagaagt
     3361 tgatttatgt ggtcatgcaa caataggtac tttccatgca cttttggaag aagggaagct
     3421 taatactaat aagagtatat tttatcaaga aacaaaagct ggtgtattat ctgtagagct
     3481 taagaaaata aataatgaaa atatatttat gatggaacag aatacacctg aatttgaaaa
     3541 tttagataat tgcagaaaag aacttgcaca aatgataggt atgcatgaag atagacttct
     3601 tgaatatcct attataaagg taaatactgg actttggtgg cttgtatttg gtctaaaatc
     3661 tttagatgat ttaaaaaata tctctccaga tttaaataaa atcaaaaaat ttagtggtga
     3721 taataatata attggtataa ctccattttg tatagaaact atagataaaa agtgtgacta
     3781 ccatttaaga tctatagcac catatgttgg agttacagaa gaccctgtat gtggaactgg
     3841 gaacgcttgc gtatcatctt atatagtaca tcacaatatt ttagataaaa ctggttttat
     3901 tggtgagcaa ggtaacttta taaacagacc tggaaaagta tatgtaaata taactagaga
     3961 aaacaatgac ataacttctg taaaaattgg aggaagtgct tatactgttt taagtggtga
     4021 aattatgtat taatcccata cctaataaaa acaaataaaa atactagcta actaaagcta
     4081 gtatttttat ttgttatttt tatttaataa tcatactcta acaaatatta taattatata
     4141 tatcataata ttgtatttag aagtaaaatt ttatttttat tattaattag ttataaatca
     4201 atctaataca acataattac agtagattaa cctataaaac ataataattg cttactgatt
     4261 atttatcgtc ctcaacaagt ctacttaatg tcttatcaga taagaataat aatatcgctg
     4321 caacaaatag tattactgga attatagtat atactttaat catatcaaat ttaagagtaa
     4381 atgcatatat atatccatat cctttaccag ctataaaagt agcaaatgtc catactccca
     4441 taagaacacc tagaagcttc ttaggtgcat acttacttac aaaagagtta ccaagtggtg
     4501 agaataacat ttcaccaaga cttaaaagaa taccaaacat gattatccat agtatactag
     4561 ctttagcagt ttcaggagct ccaactcctc ttgaaaattc tgcaccaaca agcattaaga
     4621 atgcaagacc taagaatatt aatccaagac ctgtcttttt aaataaactc atatctcctt
     4681 gtggtctact agcaagttta aaccataatg ctccaagaac tggtcctaat actatacata
     4741 caagtgaatt taatgagtca aaccaagcaa gaggaacatc aaatcctcca acattcatgt
     4801 ttacaaatgc tgcaccataa tcataaactg caagataagt tagataccag aatacccaga
     4861 aaattacaga gaagaatgat ataagtataa tagcccaaat tctcttctta tccatgcttg
     4921 taagaggaga cttatctttt tcttcaactt tttcagcaac tacaccttct ttaaaaggtc
     4981 tctttcctgc attcccaagg aatctccatc catatatgaa ccatattgca cctattacac
     5041 aaagtacagc tgctataaag aaacattgag aaaatccaag tacaccattt tttgcaaatg
     5101 ttttaaggta aagaatacca actgctatag taccaataaa agaacctata ttaacaaaag
     5161 aatattgtac tgaaaatgct gtatcaagtt cttcttgact atcaaaaagc tgtccattta
     5221 ccgcagatac gttaccttta aagaatgcag taccaatcga aactaagatt atcattgcat
     5281 ttaccataga aactgtagtt gcaatagaac caagatagta tcctaccccc atgataagca
     5341 tacctatagg tatagtatat ctagctccta tccatctatc tgatatatat cccccaatta
     5401 taggtgcaag gtatgtgaat gcaacaaggt tggcttgcat tatagcagct tgtcctttat
     5461 ctattccaag accaccatga actatagctg ttgtaaggaa tatatatatt aagtactttg
     5521 aagcataata tgctgctctt tcaaatgaga aagttgttga gcagatataa aatcccatag
     5581 gaaatttacc cttctttttt actgttgagc tcataatttt aaatcctttc ttaattgtat
     5641 ttttattttt tacgttttgt ccaaattctt tcttttatga aagatataat attattattt
     5701 tgtaaaacaa tttgtaatta ttattaagat aattgcgcat ttatttgtag agtacatatt
     5761 ctcatggaat aaaattaata cacttcttaa tcataaattt aaattatata aattcactat
     5821 tttcagatat agttataaat taatatattc tcatctaata ccatgaaaaa accttcgcca
     5881 tatgcaattc ttatttttta cgtgatagta ttttacaaac taaaatcccc cttttgtagc
     5941 aacttatgta attacatttt agtgcttttt taaaggagtc tcgaataaag caacatatat
     6001 tttgttcaaa taatgttttt atttattttt taaatttata tattacttca cccaaccaat
     6061 tctcaactta aataactaaa ctactatcta ttcacagatt aggcttattt cttacacatt
     6121 agtttataat aaacactgtg aatctaaaca taaaaactgt tattttaaag taattaccca
     6181 tatattcgct ttgatactat ttattgaggt tatactcttt atgaataata tattatttat
     6241 aaaatacctc aaaatatatt attcataaaa tacctcaaac tgaaaacttt ttcacgaata
     6301 tttatttttc acctcccctt ttttatttca ccatgtaaat cgtaccataa atatgcactt
     6361 atatgcaaga aaaaaatcta ttaatcgaca gtaaataatt agaaaaattc aaattatatt
     6421 tatcaagtat attatataaa atatttccta tgaaaatata aattaaatta atgttcagaa
     6481 tagtttttag atatgaaatc ttgatttttt tattacttta cttatttgct caaatatatt
     6541 ttt
//
LOCUS       DAEMHW010000197         6482 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEMHW010000197 DAEMHW010000000
VERSION     DAEMHW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.197"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1519)
                     /locus_tag="KPK67_003212"
     CDS             complement(<1..1519)
                     /locus_tag="KPK67_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG2460136.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSIESCLNALNEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFK"
     gene            complement(1846..2454)
                     /locus_tag="KPK67_003213"
     CDS             complement(1846..2454)
                     /locus_tag="KPK67_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBG2460137.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKCLA"
     gene            complement(2714..3259)
                     /locus_tag="KPK67_003214"
     CDS             complement(2714..3259)
                     /locus_tag="KPK67_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG2460138.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(3377..5200)
                     /locus_tag="KPK67_003215"
     CDS             complement(3377..5200)
                     /locus_tag="KPK67_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460139.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVREMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGVLASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDISSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEDVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(5213..>6482)
                     /locus_tag="KPK67_003216"
     CDS             complement(5213..>6482)
                     /locus_tag="KPK67_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460140.1"
                     /translation="ILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDY
                     ENQSFEELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGI
                     IVAYVNYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEK
                     DLKFVNCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQ
                     KLYIIDKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKED
                     VVKSCEIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDC
                     FSAMDINTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVV
                     GFATHEDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         2155 a         1139 c          584 g         2604 t
ORIGIN      
        1 ctttaaaaat cacaccaaat ggtactttat aaggtttgtc aatagtatta gaatatatac
       61 aatcacttaa ttcttcttca gtaattttgt ctattgattt atttaaaata gacactaata
      121 tagggtcagt tttatttgat acccctatac gcatcccata gcttgtatct aaaacagata
      181 taacagataa attctcactc tttccttctc ttaaaagttc atcaaactga taagaactaa
      241 gtatcattaa gtttgcttta ccttcattta gagcgtttag acaactttct atactcttat
      301 aagatgttgt cttagcattt tcaaaactct tttctatata agaatcaata gacttataac
      361 tactagttaa agcaatattt aaatcattat tgagattggt atccaatttt cctaccataa
      421 tcattggaat atttatgtat ggatttgtca atttcatatt aaatcgtttt gccttatcat
      481 tttcattgat tgagccacat aacatagttg ccttaccact ttttatataa tcaagactct
      541 cattataatt ttttgtttta atatattcaa attttatacc acattttttt gatataattg
      601 acattaaatc ggaacttata cctttaaatg aattgctttc tttatcataa tactctacag
      661 gagaccactc tgaatcatat accatagtaa gttttgggtt tgctttaata aaattcattt
      721 cttgccgagt aaatgctatg gaattattga cctctctttt gaaatatttt ttatataatt
      781 ccaattcata atacatatca tttattttta attcttttaa agcataatct aattctttta
      841 ccaaattagg tttatctttt gcagtagcaa aataaaaagg ttctactgaa aatttgctaa
      901 ctattttcat tcctttagca tttataatac ttccacaaac aaatgcatct gccttttttt
      961 cattgacgga ctcaattaat tcatcaatag aattgcagta tatatattca actgaaaaat
     1021 tattttcttc tctatacttc tcaaatgcag tattatgaaa gtttcctcta agtacagcta
     1081 ctctcattcc cttaaaggaa cttatatctt cataaaacat attattgtta ctatcctttg
     1141 tatatagtgc actatattca tatcctgaac ttagtttagg aaaattaaat agtttatttc
     1201 tttcatcatt cttctgtaaa ggtccgagta agtctatttc acccttttct agtctgtcta
     1261 aacattcttc ccaagaccct tttacaaatt catattccca tcttgtatat tttgagattt
     1321 ctcttagata atcataacca tatcctgtat aagaatgctc ttttgatgag gatattattc
     1381 cgtagtcatc acaatatcca actcttattt tttttgaatt ttcagactca tcagcaaagc
     1441 atagtatatt gttaaaacat gatataataa gtaaaaatat caaagaaagt atcaagaagt
     1501 gctttttaaa tgctttcata tttaaaaacc tccaatttgc caacttactt taacttattt
     1561 ggttttctag ttgtaactac ttttattgta atggtaaata tatcaaattg caatattctc
     1621 atataatatt cttattcaac tataatgaat ttattattat ctatttaaat acactatcta
     1681 atattagttt cttataagtc ataaaagcac aactaacact ataattctag tgattaattt
     1741 tatttcaaaa aacataataa cactactaat attttaaata tcaatagtgt cataaaaact
     1801 tttcagatta attaaatctt tctatatatt ttataataat ctaaatcatg ctaaacattt
     1861 agacagaaaa cttttttctt taatagtaaa tacttttgtt tgaatatcag aataattatt
     1921 tgaatatcta tctataaaac catgatttgc atctaaaatc tcaatttgga tattttgttt
     1981 cctagaaagt tggtaagaaa tagaatcaac atcaaatgaa tcgtgctttg caaatactaa
     2041 taaggtagga cattttggaa ttacatccat gtaatctcga atccgagagc cataacaaca
     2101 aattatgcca tcacaaaaag aatcttcact gcatctccat gcaatagttg ctcccacgct
     2161 atacccagca acaaatacat atttatactc tgattttaaa tcatttataa gcttttcaac
     2221 ttttttataa atatcaaaac caacattatt tataaaaaaa tcatatgcct cttggacttt
     2281 tgaatatgga aaaactcttt tttctcccag aagttcagga caaaatacat catatccata
     2341 tttataatac ttttgacaaa catcttctat aaagctatta atcccataga tttcatgcaa
     2401 aacaacaata gctacatcac ttttatgtat atactttatc agattatcca ccacctttac
     2461 aaaacagcaa attttttata cttagtttaa tatattcttc tttacatgtc aatttacttt
     2521 tctaaaatca aatttatttt tggattaatc atttattttt aaaagttgtg cattattact
     2581 attaaattat tctctattat taaaaatata atcgttagaa ataaaaaata aagtacaaat
     2641 aattaaaaaa tatctcactc ataactttaa aaattataag tgagatactc ctaaaatcta
     2701 ctttatatta aatttatttt ccaaaatatc tatttaataa tcctaagaat gctttaccat
     2761 gtctagcttc atctttacac atttcatgta ctgtatcatg tatagcatct aatcctaatt
     2821 cttttgcttt cttagctaat tttaattttc catcagttgc accatactca gcatcaactc
     2881 taactcttaa gttttctttt gtatctgcaa ctacaacttc tccaagaagt tctgcaaatt
     2941 tagcagcatg ttcagcttct tcaagagcta ttctcttata agcttctcct acttctggat
     3001 aaccttctct atcagcttgt ctactcattg ctaaatacat tcctacttct gtacactcac
     3061 caacaaagtt agctcttaat ccttcgatta tttcttcatc tacaccttga gctactccta
     3121 ttctgtgctc atcagcccaa tttttttcgc ctttcatttc ttcaaattta tcagctccaa
     3181 ctttacatac tggacattgt gctggtgcag catctccttc atgtatatat ccacatactg
     3241 tacaaacaaa tttcttcata attactttcc tcctaaattt tatatatcaa attaaatttt
     3301 ttatttatta agttagtcta tatatacccg caccatatac ctttaaacaa tttttttaaa
     3361 agttttttct aaatacttat tttccctgtg tcaataatat gtttctataa taatgtccct
     3421 ttaattttat caattcttta tgatttcctg attccacaat tctaccattt tctaaaacta
     3481 ttatattatc acaatttgat atggtactta atctatgagc tataattata cttgtctttc
     3541 cataagttaa attatttatt gcttcttgaa ttttagcttc tgttgctata tctatgttac
     3601 ttgtagcctc atccaaaata agtattggag cattttttaa tactgctcta gcaatactta
     3661 ttaattgttt ttgaccttca cttaaatctt cacccctatc cgataataca gtactatatc
     3721 cttcacgcat tttaactata aatttatgtg catttgctat tttactagca tatataacgt
     3781 cttcatcagt agcctccaga tttccatacc ttatattttc cataactgta tcattgaata
     3841 tataaccttc ttgtggaact actccaaaac aagttctcaa atagtttctg ttaatagagg
     3901 atatatcctt accatctaaa aatatacttc cttcatctat atgataaaaa tttgtaatta
     3961 gatttataat tgttgttttt ccacttcctg tctctcctat gatggcattt gatgtgccag
     4021 ctttgatttt taggtttata ttatctaaaa ttttcttttt tgaatcataa gaaaaactta
     4081 catctttaag ctcaatttct ccctttacaa aatgagagtt taaattacca tctttattat
     4141 cgttaatctc acttttaaac atgttatgca cttcttgctc ttcttgttca tctataattt
     4201 caaaaactct ttcacatcca gctatggctg aaagtaatgt attaaataga tttgctaatt
     4261 catttaaagg tcttgtaaat tgtcttacat aacttaaaaa actagctata acccctatgg
     4321 ttataatatc tttagatgca agaacgcctc ctataataca tatggttgca aatcctatat
     4381 tactaatcac attcataata ggcattaaaa gagaagtgta aatttgtgat ttaagggaaa
     4441 tatttagaag tctatcattg acttctttaa aatcatttac agattttctt tcataattaa
     4501 aagattttac tacatttatg tttgatatca tttcttcaat atgtgcattt agttttccca
     4561 actcaacttg ttgttgctta aaaaaaactc tagttctctt tgcaataaaa ttactcacaa
     4621 acattactat cggtattaat attacactgc aaaaggttaa aattgggctt aatacaaaca
     4681 tcataataaa agtcatcaat atgtttaaga ttcctgtaat taactgtact aatgaatttg
     4741 atattcctgt actgatatta tcaacatcat ttgtcactct actcataata tctccagtag
     4801 aacttttatc aaaaaaggtt attggtagat ttttaaattt taaaaataaa ttacttctca
     4861 tttctctgac tatactttga gataatctag ctgtacatat ccccttagac agtttaactg
     4921 aaaactcaat tatataaaat gaaagtaaaa taattattcc ttttaagaaa atggattttg
     4981 aataagtatc gatatctatt atgtcaacta cctttccaat agtatatggt attacagttg
     5041 atataattga atcaattatt actagcaaaa aaataaatct agtaatcttc ttatcttttt
     5101 taaagtacat aaaaattctt ttaaacgttt ttttaaaatc tttaggcttt tcttttttct
     5161 ttaatctact gaccttctga tttccagtac ttaaattcaa tatacttcct ccttaattta
     5221 catcttcact tactaaattc tgcaacgcaa aaattttttt ataaattgga gaactaacca
     5281 gtaaatcttc atgtgtagca aatccaacca cctcaccctc atccaagact aaaatcttat
     5341 cacagtcaat tatagaacta atcttttgtg aaatagttat tacagtacat ttcttacttt
     5401 ccttttctct taaattactt atatactctt ttatattctt tcttataatt gtttctgtat
     5461 taatatccat tgcactaaaa caatcatcaa gaattaatat ttgtggtttt ttggctatag
     5521 ctctagctat agtaattctt tgtctctgtc ctcctgataa atttttaccc ctttggaata
     5581 aaacttcatc ataagtattt tcgagttttg ttataaattg atttgcattt gctatttcac
     5641 atgattttac aacatcctct ttgcatatat ctctatttcc cataactaca ttctccaata
     5701 tactttttcc aaacacacta ctattttgaa atactacacc tattcttgcc cttaaacttt
     5761 cttcagaaat actgtatata ctacgtcctc caatcttaat atcaccttta tcaattatat
     5821 ataatttttg aattaaattt actaaagaag tctttcctga accagtttga ccaatcacac
     5881 caatattttg accttcttct attttaaagg atacgttttt taatattttt tcatctgtat
     5941 ttttgttata cgaaaaatct acattttcaa aaattatatc acaatttaca aactttaaat
     6001 ctttttctcc aacatctaaa ctttcttctc tctcaattac ttcttcattc tcatcaacca
     6061 aaacctcact tattctctca taagatgctt ttgccctaac aaaaacatta aaaacatgtg
     6121 aaatcattat aagagatgtt aatattctag tcatatagtt tacataagca actataatcc
     6181 caattttaat ttctcctgaa taaattaaat gccttccaat cagtaatatt attattatgg
     6241 aaacattaat gattattgta attgtcggtc taaaaaatga catcatagtt gtagcttttc
     6301 tactattttt aaaaagttca tcatttaact cttcaaagct ttggttctca taatcgtatc
     6361 tacaaaatag tttaactact tttattccac ttaaaaactg tcttaatttt gaatttactt
     6421 tgtctgtact ttcttgtaca tttttaaata atctataccc tatcttcata ttaagcaata
     6481 tt
//
LOCUS       DAEMHW010000198         6428 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEMHW010000198 DAEMHW010000000
VERSION     DAEMHW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.198"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            401..1345
                     /locus_tag="KPK67_003217"
     CDS             401..1345
                     /locus_tag="KPK67_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBG2460141.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1367..1993
                     /locus_tag="KPK67_003218"
     CDS             1367..1993
                     /locus_tag="KPK67_003218"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBG2460142.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            2027..3076
                     /gene="hisC"
                     /locus_tag="KPK67_003219"
     CDS             2027..3076
                     /gene="hisC"
                     /locus_tag="KPK67_003219"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG2460143.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRDKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            3079..3657
                     /gene="hisB"
                     /locus_tag="KPK67_003220"
     CDS             3079..3657
                     /gene="hisB"
                     /locus_tag="KPK67_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBG2460144.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3659..4264
                     /gene="hisH"
                     /locus_tag="KPK67_003221"
     CDS             3659..4264
                     /gene="hisH"
                     /locus_tag="KPK67_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBG2460145.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4261..4983
                     /gene="hisA"
                     /locus_tag="KPK67_003222"
     CDS             4261..4983
                     /gene="hisA"
                     /locus_tag="KPK67_003222"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBG2460146.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4965..5723
                     /gene="hisF"
                     /locus_tag="KPK67_003223"
     CDS             4965..5723
                     /gene="hisF"
                     /locus_tag="KPK67_003223"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBG2460147.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5751..6419
                     /locus_tag="KPK67_003224"
     CDS             5751..6419
                     /locus_tag="KPK67_003224"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBG2460148.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2629 a          541 c         1257 g         2001 t
ORIGIN      
        1 ttttttattg tataaacaaa agttatatat aaaatatttt attcaatttt ttaaataact
       61 attgccaatt tttgaaaaat atattattat taatagtaac aaaaaactgt aaatttagaa
      121 tattaataaa aattatttgg gaggaaatta tcatggtgag attaaatata gtaaaattaa
      181 atacaaaatt aaatcatcac catcatcata gattaaaggg attgtaatat gaaattaatt
      241 catagacaca agccctgttt ttagtgtaat tggtcttgta tctatgatgg gaagtaattt
      301 taagagaaaa ttatagccat ataggtataa agcctatatg gcttttttat tgttttaaat
      361 ttgcaaatat aagagtaaat ttaatgaaga gggtgagaat gtgaaatttt tgaggattga
      421 agatgaggtt atttattcta aaaaaagata taaattaaaa agggaaatag ataaactttt
      481 tttggatgaa gaatattttc aaatagaacc acaattattt gaagaatatg atgaatttac
      541 aactataaat aataaaattc cagaagaatc tatggtaaag gttgtaaatg gaaaagtaat
      601 ggtacttaga gctgatataa ctacaagtat aataaaaagc cttgtaccaa gatgggaaga
      661 tggtttaaag cttaagcttt tttataattc atcaatatat aaaaataaaa atacagttgg
      721 aataaaagag ataagacaaa taggttgtga gtatctagga gaagcctctg tagaagcaga
      781 tagagaagta gtgaaattag ctcttaagat acttgaaaaa tacaataata attttattct
      841 agaggttggg agtagcaagt atattcatgg tttgttagaa gaattaaatt taaataagaa
      901 ttgtgaaaat caaattaaaa atttactata tacgaagaac actcatgaat taaaagtata
      961 tattgaagaa ttgaaaatta agcgtgaagt aaaagagtta ttatcaaata ttttaagttt
     1021 acaagggaat ttatataatg ttattgaaaa atcaaacaag ttctattgta ataataagat
     1081 gaagcaagct cttgaagaat taaagcaagt aaataattta attgaggaat gtaatttttt
     1141 agataaggct agatttgatt tatcaatgat tactatgctt gactattatg aaggaattat
     1201 gtttagagga tactacccaa actcatataa agaaattctt agtggtggaa gatatgattc
     1261 tttaactaaa gagtatggaa aagaaattcc agccattgga tttacactaa gtgttgatga
     1321 gttaatgaag tatgtatata agtaaagttt agaaggaggg attgctttgg attatttgac
     1381 tatagcacta gcaaaaggac gaattgaagg ggaatcattt aaaaaattta aaaaaatggg
     1441 tctaggagat agtatagaca cagatacgag aaaactaatt tttaaagatg aagaaaataa
     1501 aattatatat atacatgtaa agccatcaga tgtagttaca tatgttgaaa aaggagtcgc
     1561 tgacctcggt atagcaggta aagatactat tttagagaat gagacagatg tatatgaaat
     1621 atatgattta ggttttggaa aatgtaaatt tgctgtagca ggattaaagg gagatagcat
     1681 atatagagaa gatgaatatt taaaagtggc aacaaagtat cccaatatag caaaaaagta
     1741 ttttaaagag aaaggtcaaa aaattgaaat aataaaatta aatggttctg tagaactagc
     1801 tccaatagta gggttgtcag atgtaattgt agatatagtt gagactggca atacacttaa
     1861 agcaaatggt cttgaaatat tagaagacat atgtaatata agtgctagaa ttatatcaaa
     1921 tagagctagc tatagattta aatatgaaca aattcagaat ataattcgtt tatttgaaga
     1981 acttgataat taaaatataa ttgagcttta taaggaggga tttaagttga gagaaaaaga
     2041 aagcataaga gagttaaggg gatatgaacc aaatcacgta aactgtaaag taaaacttga
     2101 tgcaaatgaa ggaagtaaga ggttgtttaa ataccttatt aaagaaattt cagatagtga
     2161 tatagattta aatttatatc ctgaagattc ttatagtgat ttgaaagaat ctataataga
     2221 ttacataaat atttctggag taaataagaa aaatcttcta gtaggaaacg gttcaagtga
     2281 aataattgat ttaataatac atacttttgt agataaagat gaagtaatat tgagtttctc
     2341 tccaagtttt agtatgtatt ctatatatag tcagataaat gggtctaaat ttataggtgt
     2401 tgaaagtgat gaaaatttag ttataaatat agataatgtg attgaaaaag taaaggagaa
     2461 taatcctaaa atagtaattg tatgtaatcc taataatcca actggaacaa tattaaagag
     2521 ggaagagata ataaagcttt tagattcaac taatagtcta gtggttttag atgaagcata
     2581 tatggatttt ggagaagaaa gcatgttaag tgatgttttt aagtatgata atctaatagt
     2641 tcttagaact ttatcaaagg catttggatt ggctggaata agaacaggat atatgttatc
     2701 taatagtagt ttaataaatt ctgttgagaa agtaagacct ccatataatt taaattcatt
     2761 atcagacttt attgctacaa gagcacttag agataaagat gtggtaaagg cttatataaa
     2821 ggaagtaaaa gaggaaagag aagttttata tgaagaaatg attggtatgg gaattaaagc
     2881 atacaaatca caagcaaatt ttatattatt ttattctgaa atcgaaaatt tatcacaaaa
     2941 acttattgat agaggtgttt taataagaaa atttggtgga aagttagaaa attattacag
     3001 agttacaatt ggtgataaag aagaaaattc tatgtttgtt ggagctataa gagatatttt
     3061 gaaaaaggag aaataggtat gagaatttgg aaggtagaaa gaaatactct tgaaacacag
     3121 attttagtag aattaaatat tgatggttct ggtaaagctg aaatagatac tggaatagga
     3181 tttttagacc atatgcttac cttaatgtca tttcatggca agttcgattt aaaagtgatt
     3241 tgtaagggtg atacatatgt agatgaccac catagtgtag aagatatagg tatagctata
     3301 ggtgaagcat ttaaaaatgc actaggtgac aaaaaaggaa tacgaagata ttctaatatc
     3361 tatataccta tggatgaatc tctatcaatg gtagcaatag atataagcaa tagaccttat
     3421 cttgtattta atgctaaatt tgatacccaa atgattggta gtatgagtac tcaatgcttt
     3481 aaagaatttt ttagagcttt tgtaaatgaa tcgagagtaa cattacatat caatctctta
     3541 tatggggaaa atgaccatca taagatagaa agtattttta aagcatttgc aagagcttta
     3601 aaagagggta gtgaaatagt ttctaatgag atagcatctt caaagggggt tttgtagaat
     3661 gaatataata gtagactatg gtcttggaaa tatagattct gtgtctaggg gttttagaaa
     3721 agctggaata gaaactaaaa tttctagtga tatagatgaa ataaaacagg cagattcact
     3781 tatacttcca ggtgttggag cttttagaga ttctataagt gccttagata aactaggatt
     3841 gataccaata ataaaggagc atgtttctaa gggtaaattt atgataggta tatgtctagg
     3901 aatgcaatta ctttatgaaa aaagttatga gtatggagaa tatgaaggat taggacttat
     3961 aaaaggaagt attgataaac tggatataag tttaaaagtg ccacatatgg gatggaataa
     4021 tctaaaattt aataaggcaa atgatgatat acttaaatat ataaatgaag atgattatgt
     4081 atacttcgtt cactcatatt atgcaaattc atcaaatgaa gaactaattg cattttcaga
     4141 atatgaaaag aaaatccctg ctattgttag aaaaggcaat gtatatggta tacaatttca
     4201 tcctgaaaaa agtggagaag tagggttaaa tatattaaga gcatatgggg agatgataaa
     4261 atgataatat ttccagcaat agatataaaa gataataagt gtgtaagact cacacaagga
     4321 gaatttgaca aagtaaatgt atattatgat aatccattag aagtagcata taaatggaaa
     4381 aatgaaggag cagaatatat acatatagta gatttaaatg gtgctagaag tgaatttggt
     4441 gtaaatacaa aaataattga ggacatagca aacaatatag atatacctat tcaagttggc
     4501 ggtggagtta gggataagga aaaagtcaaa agtttaataa atgctggtgt tactagagta
     4561 atattaggta gtatagcgat tgaaaatttg aatttagttg aagaattggt caatgaatat
     4621 aaagagaaaa ttgtagtttc tatagatgct aaagatggaa aagtagcagt taggggatgg
     4681 gaagttgtaa gcaatgtaga ctcattaaca ttatgtaaac aacttgaaaa gataggtgta
     4741 caaactattg tttatacaga tatatcaaaa gatgggatgt tgcaaggtcc aaattttgac
     4801 atatatgaaa gaatagcaaa ggaaacatca ctaaatgtta ttgcttctgg aggcgtaact
     4861 tctatagaag acgtaaaaag actaaaagct atgaacttat atggtgcaat aataggtaaa
     4921 gcgctttatg ataaaaagat agactttaag gaggcacaac aattatgctt actaggagaa
     4981 taataccttg tctagatgtg agaaatggaa gagtcgtaaa gggaaaaaaa tttaaagata
     5041 tagtagatgt tgatagtcca gaagtacttg gaaaatttta tagtgattgt ggtgcagatg
     5101 aacttgtatt ctatgatata actgcatcaa atgaagagag aaagacatct ttagaatttg
     5161 tgacaaaagt tgcagaaaat ataaatatac cattttgtgt tggtggagga gtaaataaat
     5221 tagaagattt cactgatatt ttaagaaaag gtgctgataa agtttctata aattcatcag
     5281 cagtcaaaaa tcctgaatta ataagggaag cttcattaaa atttggagca caatgtgttg
     5341 ttttatcgat agatgcaaag aaaaatgaag agggttcatg gagcgtatac gtaaaaggtg
     5401 gaagagaaaa aactaatctg gatgcaattg aatgggctgt taaaggtgta gaacttggag
     5461 caggagaaat cgttgtaaat agtatggatg aagatggaat gaaaaatgga tatgatatag
     5521 aattattatc aaagataact tctttagtaa atgtacctgt tatagcatct ggaggcgctg
     5581 gaaaaaagga agatttttat gaggcggtta ataaatccaa tgtagatgga attttagcag
     5641 catctgtttt tcattttggc gagataaaaa taaacgattt aaagaaatat ttaaaagaca
     5701 tgggtgtaga agtaagatta taagttaaat actaaaagga gattagaaat atggataata
     5761 agtgtaacaa tgtatacagt gatgaagtag agaaatttat aagaagtata aagtttgatg
     5821 ataaagggtt agtccctgtt gttgttcaag aggtagttag caaagatgta ctgatgttag
     5881 catatatgaa taaagatgct ataaagaaaa ccttaaaaga taaagtagct tgctatttta
     5941 gtagaagtag acaagaactt tgggttaaag gtgagacatc tggtaatact caaaaagtag
     6001 ttaagatgtc ttatgattgt gatgtagata ctatactact ttttgttgaa caaactggtg
     6061 tagcttgtca tacaggcaat tattcttgtt tttacagaga cttgtttgat gatagttcaa
     6121 aaatggagct tgaagtacaa acaaatatat tgaaagaatt atatgattta atcaatgaaa
     6181 gaaaaaataa tccagttgaa ggttcttata ctaattatct ttttgaaaaa ggaatagata
     6241 agattcttaa aaaagtaggt gaagagagta gtgaggtaat tatagcatca aaaaataccg
     6301 ataaaagtga acttatttac gaaattagcg atttagtata tcatacattg gttttaatga
     6361 tagaaaaagg tgttgaaatt gatgaaataa aaaaagaatt acttaaaaga agaaaataga
     6421 ataagata
//
LOCUS       DAEMHW010000199         6397 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEMHW010000199 DAEMHW010000000
VERSION     DAEMHW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.199"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     repeat_region   452..659
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=452..480
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaat"
     gene            complement(827..1111)
                     /locus_tag="KPK67_003225"
     CDS             complement(827..1111)
                     /locus_tag="KPK67_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460149.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     gene            1318..2136
                     /locus_tag="KPK67_003226"
     CDS             1318..2136
                     /locus_tag="KPK67_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2460150.1"
                     /translation="MEKQCFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            complement(2269..4389)
                     /gene="feoB"
                     /locus_tag="KPK67_003227"
     CDS             complement(2269..4389)
                     /gene="feoB"
                     /locus_tag="KPK67_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG2460151.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(4415..4642)
                     /locus_tag="KPK67_003228"
     CDS             complement(4415..4642)
                     /locus_tag="KPK67_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG2460152.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            complement(4969..5409)
                     /locus_tag="KPK67_003229"
     CDS             complement(4969..5409)
                     /locus_tag="KPK67_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2460153.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGVICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     INLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            5561..>6397
                     /locus_tag="KPK67_003230"
     CDS             5561..>6397
                     /locus_tag="KPK67_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBG2460154.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RIL"
BASE COUNT         2375 a          824 c          852 g         2346 t
ORIGIN      
        1 tcataaataa aataataaga ggtagataca gttttaaggg aatacaaaag tttttaatta
       61 aactatgctt gttcagatag atatttattt aagaaaaaag actattaaaa gcaatataca
      121 agaatgatat attagattga ttaaacaagc ataaatatta tgtaaaaaac tttaagttat
      181 agaatttaaa tctaatgtag atagattacg tttttttgct tttattatgg tataaattgg
      241 tatcaatatt caaaagtaat atatttatga tataataaaa ttatagaaat tttgcagtga
      301 gcgatatttg tgaaaaaatt tggcgtaaca gttgaaatat aaggcgttga gagtgcatga
      361 taagcgttat caattgcact attgctcgtt cactgtaaat ttaggagagt tgtatacgtg
      421 taagtgttga aaatactaag tttattttgg ggttttatat taactatatg gaatgtaaat
      481 agaaaatagg ttgcatattg aatgaaattt ttgcaagttt tatattaact atatggaatg
      541 taaattcact aacaggaaca tcaaaaggag gtttaaaatc tgttttatat taactatatg
      601 gaatgtaaat ttttcatcta ttgcattaat gtaataagat aactatacgg aatgtaagtt
      661 tttataattt tcttttaatg tgttatctta tgattatcta gttatattag catacaaaaa
      721 tataataaaa ttacactata ttataaaaac aaaaaggtag tatagaaatc ctattacctt
      781 tttattatta attttatcag tgttttatta taaatagctt acataattac acattttttc
      841 tgattaaata atatgatgct attgttataa tagatactaa tgctaatgat gttattgcag
      901 tatctaatct tcttattaat aaactcattt ctatatattc aagttctatt aaatattgat
      961 aaaatatttc tagtttaaca gctttttctc tatctatatt tccatacttt aactctaatt
     1021 tatctaaaga ttttttatta caataattaa ctttatgttt aattaaatat cttattgtag
     1081 cacctattac aattttcact ctaacaagta taaatataat attccatcca aaagttaaga
     1141 ggggatatct tttttatgat gggaatgggt aaaatattta taactgacat ctaactgata
     1201 tttaagcttt aaaatcaaaa ccatattgat tttctaaaat aataactact tgatttagta
     1261 aataataaag aatatagtaa ctatatagac aacaaaaaga aaaggagtgt ataacccttg
     1321 gaaaaacagt gttttacaac aggtgaattt gctaaattgt gtggtatatc taagcagaca
     1381 ttaattttct atgacaaaat aggtattttt tctcctgaat ataaagataa aaataattat
     1441 agatattact cagtttatca atatgataca cttgatatat tacaatcatt gagagaaata
     1501 ggaatgagtt tagaggaaat aaaagagtat atacaaaata gaactcccca gctttgtgtg
     1561 aaaatgctta aagaagaaga aaaaaagata agagaaaaaa taaaaaaact aagaaagata
     1621 agttctaaga tgcaaaatag aataaatata acagttgaag gaatttctaa aatgaatagt
     1681 gaagaaattt atatatccaa aaagtttgaa gaatatttag ttctatcaag cgatttgagt
     1741 aatatgagta atagtgattt tatgatggaa ttaatagatt ttaccaatta ttgtaaatcg
     1801 aaaaatttgt atcaaggata tgagttggga gtaatagtaa gtaatgaaaa tataaaaaat
     1861 ggggactatt taagtatatc aagtttttat ttgaagatag ataaaaaaat aaaggataaa
     1921 aagctatatg taaagccaga aggaatgtat gcttgcataa aacatattgg aaaatatgag
     1981 gattcatata aatcttacga aaaattgaaa aaatatatat atgaaaatga atataaaatt
     2041 gttggaaatt cgtatgagga gagtatactt gacttttttt gtgaaagtaa tgaagataat
     2101 tatatgactg aaatttcaat ccaagtatcc agataagaat taaaatagag atatattcaa
     2161 atgttaagta attttactta tcattgaata tatctctatg ctttataata aagtatataa
     2221 atttttaaat agtttctcta atatatgtct actgtcagtt aatactaatt atataaataa
     2281 attgtaaact gtagttgtta aaaaacaaac aacaaaagca agtatagtag gcattaagaa
     2341 tcctaaggca gtccatttta aactaccagt ttcttttttt attgttaaaa gtgttgttgc
     2401 acaaggccaa tgcagtaaac tgaacagcat aacatttaaa gctgtcaaat aagtccatcc
     2461 atgttctcta agaacttgac ctaaagcact aaagctatct aattctatca tagaaccagt
     2521 tgctaggtaa gccattaaaa gtataggtac aactatctca tttgctggga atccaagaat
     2581 aaaagcaagt aatataaaac catcaagacc aattaattta gctaaagggt ctaaaaagtt
     2641 tgcaacatgt gataatatac ttaaatctcc tatatatata tttgcaaata tccaagttat
     2701 gacaccagca ggtatggcta ccattacagc ccttcctaaa acaaatattg ttctatctat
     2761 tatagaggta tataaagttc ttcctatttg aggaactcta taaggaggaa gttctaatgt
     2821 aaatgtagac ggaactcctt ttaatagcgt tttagaaaga gtataagaaa ccaacaatgt
     2881 tattataaca cctagaatta tcagtaaagt tatgcaaagt gcagttgcta cgcttgatac
     2941 gaaagagttg gttataactg agctaaaaaa tattgtagat atagctatca aagtaggaaa
     3001 acgaccatta caaggaacaa agttgtttgt aagtatagct ataagtcttt cccttggaga
     3061 gtcaattatc ctacaaccaa taactccagc tgcattacat ccaaatccca tacacatagt
     3121 caaacactgt tttccatgtg cacaagcttt tttaaatagg tggtctaagt taaatgcaac
     3181 tcttggcaaa tatccaaaat cctcaagtag ggtaaataat ggaaaaaata ttgccatagg
     3241 aggtagcatt acagagatta cccatgctaa tgttctgtat aatcctaaaa ccaacatatc
     3301 atttaaccat gatggacaat taatactatt taatataccc gaaatacttg gctcaaatcc
     3361 gagtaataaa ttagataata aagtggatgg atagttagct ccttctatag taatccacaa
     3421 tatagtacct aataataaga gcattattgg aagcccaaat attttagatg ttaatatttt
     3481 atctactttt tcttctctat cagttgattt tttagcaaca ttagaacaac actcatcact
     3541 cagtttttta gcataatcat agttgatttt agtaaactca tctcttattt tctgtttatt
     3601 tatattgtct ggaatctttt tctttacttc attaatagca tctatagaat ctttgtctat
     3661 ataattactc atagattcaa atatactttc atcgccatca ataagtctaa gtcccaacca
     3721 cctagaattt atacctggta taatattatc caattctggt tgaattgatt ttaccacatt
     3781 ctctatattt tcattgtatc taacaggttt attatttaat ttatatttgt caaaagatac
     3841 atcatttaaa gtatccaata gttcatccat accagagcca tttcttgcag cagttaaaat
     3901 aactggaata ccaagagaat cctctaaaag ttttttatca atagtaatgc ctttttttct
     3961 agcttcatct attaaattaa tacacaaaat aactttatct gtaagttcca tcacttgaaa
     4021 tactaaattt aaatttcttt caagacatgt agcatcacat actactatta ctgcatctgg
     4081 attaccaaaa catataaaat cacgagctac aatttcttct tgtgataagg caaataaaga
     4141 atatgttcct ggtaaatcta ttaatgcata ttcggtattt ttatatttaa aattaccacg
     4201 agctgtagct acagtttttc ctggccagtt tccagtatgc tgtctaagcc ctgtaagatg
     4261 attaaataca gtacttttac ctgtatttgg attgccagcc aatgcaatta caaactggtc
     4321 ttccttattg tctatatcaa acatgtcttt taaagaactc attttagttg agttatgtgt
     4381 caaccccata ttaatatcct cctgctagaa atttttaaat gtcagaaact aatattaagc
     4441 tactttcttc ttgtcgtaga gctatcttgc tacctctgat tttatagaca gttaaattat
     4501 tttttggacc ttttctaact acatctataa cagctcctct agtaagacca agcgctaaca
     4561 ttctttctct taaattacca ctagataata tatcttcaac tttgacagtt tttttcactt
     4621 gaatatcgtt aaggtttttc atagtagcac ctccatgctt gaaatttagc ctgggctaat
     4681 cttcttattt taaactatgc attatattat taccttgtta cttttacaaa aaaaagatag
     4741 gcaagatgcc tatcatacaa agttgggtat aattcttact tttggggaaa gtagaatata
     4801 gaataaaaat taacactttg aatccatata attattgaat tttgataata aaaaagtcag
     4861 aaagcaaaag ccttctgact ctatgtaagg tattttcaaa tacctaaata tcaattcaat
     4921 atttaattta tacttaaatt atatattaac aatattagct tgtcaacact ataatctcat
     4981 tttaatatgt tcaatacctg cttcgtcgta tacttctgag atttctttaa aaccacaaga
     5041 taagtaaagg ttctttatat aagtttgtgc tgataaggtt atattatttt cgtgtaagtt
     5101 aatttttata aaatctatag cacaatccat catttgtttt gctaatcctt ttcttctatg
     5161 actagaaaga actaaaactc taccaatgga aacatcatta taagagctgt attctttagg
     5221 aattattcta gcataagcac aaatcactcc attttctttt aaaaatatgt gataagagct
     5281 tttatctata tcatcataat cgtttaatga aaatatcttc tgttcacaag caaatacctc
     5341 atatcttgat tttgctattt cataaaattc atctaaactc aaatcattaa agtgtttaat
     5401 tttaaacata gtaattacct cctagcatct taaaaaactg aatattaaga tacattatac
     5461 catttttaac ataaatacct ttattaaaat ttatatttat taaaaatatt tttaaaaagt
     5521 gatatactat attaaaaaca aaactttagt tagaaaattt atgatttatg gaggcagtgt
     5581 tttgaatact actaaaaaat taactgaagc agctttattg tcatctttat tcatagtagt
     5641 aacaattata gctgtgagta ctggatttgg atatgctatt tacttagatt ttatagtgcc
     5701 agtatttttt tgtataatct gcttgaaatg tgatttaaag tataccattt tatctggaat
     5761 tacttcactt ttaataattt ctctggtact tggaaatcta ggaactgcta tttgggcaag
     5821 tcagagtgta ttacttggta taatgtgtgg ctatctaata aataagccta caatggttat
     5881 ggatgattta gtatatggtt cagtatttgg agttattgta atggttttca ttgatatata
     5941 tgcttcaaca ttaataggtt atagttttat gaaagaattt caaggatata gcaaatggat
     6001 ttattttaat gggtatacaa attttattta ttatttaatg atagcattgt ttccttttgg
     6061 tatggtgttt tgtatatatt tattaagttt aattttaggg aataaattgc atattttaga
     6121 ttctaattct ttaaaaaaat ttttaatatt taagaatttt agaagtttaa accaattttt
     6181 atgttgctca aaaaaagctt tttacatatg tgtttcttat ctagccattt ttgaagttct
     6241 aaaactgttg aatgttaaag ttgattctgt ttatttaaaa acaatcttta tatcaataac
     6301 ctatataagt gtctatttta tcattagaga ctcatgtatg tcactacaaa actttataat
     6361 agctaagttt agaaaattat tatatgctag aatttta
//
LOCUS       DAEMHW010000200         6318 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEMHW010000200 DAEMHW010000000
VERSION     DAEMHW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.200"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            255..800
                     /locus_tag="KPK67_003231"
     CDS             255..800
                     /locus_tag="KPK67_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG2460155.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTALGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            813..1826
                     /locus_tag="KPK67_003232"
     CDS             813..1826
                     /locus_tag="KPK67_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG2460156.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            2048..4021
                     /locus_tag="KPK67_003233"
     CDS             2048..4021
                     /locus_tag="KPK67_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG2460157.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNISRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            4090..4449
                     /locus_tag="KPK67_003234"
     CDS             4090..4449
                     /locus_tag="KPK67_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460158.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4484..4840
                     /locus_tag="KPK67_003235"
     CDS             4484..4840
                     /locus_tag="KPK67_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG2460159.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4877..6169
                     /locus_tag="KPK67_003236"
     CDS             4877..6169
                     /locus_tag="KPK67_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG2460160.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT         2506 a          588 c         1190 g         2034 t
ORIGIN      
        1 aatttctaag aagaatttag aatatagtcg aaataagaat tgtatatttc tgaaaattct
       61 aattagtaca attttttcct ttataaaatt gaaaaaagat gtatttattt atcaaaaata
      121 ttatattaaa atgatattaa taaaaaaaca tttacaatat tagcatatag tacagaaaaa
      181 agatgtaatt tgtaaaattt atggtaagca gtaaaattaa aaatttaaag atattatagg
      241 gaggttataa ttttatggaa aacgttatta caggtctaag taaaggtgca gagtggttta
      301 tcggtttgtt tcagaaaggg ggagaacaat ttataggtct tgtttcaggt actcttccaa
      361 cgctaattgt attaatggta gctattaact ctttgattaa gataattggt gaggaaagag
      421 ttaataattg ggcttctaag ctaggaaaga actcttttac aagatatatt ctacttccat
      481 tagtatcagt atttttcttg ggaaatccta tgtgctatac atttggtaga tttttaaaag
      541 aagagcacaa agcaggattt tatgatgcag cagtttcatt tgtacatcca attacaggtc
      601 tttttccaca tgcaaatgca ggagaacttt ttgtatggtt gggaatatca gcaggtttga
      661 ctgctttagg taaagaaact actacactag cattgtggta ttttatagtt ggtctaattg
      721 taatttttat aagaggtata gtaacagaaa aaatgtatgc ttttctaaca agaaaaaata
      781 gagcaacaaa taaggcttag gaggtagata aaatggagga gaataaaata ttaaaaatag
      841 aaaagggaac ttcaggctgg ggaggtcctt tgtatataaa aaaagaaggg aatagaaata
      901 agatactatc aatgactgct ggcggtatcc atgaagtaac attaaaaata aaagaactac
      961 tgggttgtga aattgtagat ggatttaaaa ctggagtttc tgatgaagaa gtagcagtag
     1021 ttattattga ttgtggtgga actgctagat gtggagttta tccaaagaaa aaaataccta
     1081 caataaatgt aaatccagtt ggaaaaacag gtcctttagc aaaatttata acagaagaat
     1141 attatgtatc agatgttaat cctaactgta taagtgttgt agatggggaa gatatgccac
     1201 aaaaatctca agaaaataaa agcgaaaata agagttctat tagaaagcct gataattatg
     1261 atgaggttaa gtcaaaagca caaggtgaat atgctaaaaa gaacataata ctttctattg
     1321 gtcaaggggc agggcaagta gtatcaaaat tttatgatgc aggtcgagat actatccaaa
     1381 tggtaatgaa taacgtaata ccatttatgg catttgtaag tatgcttatg ggaattattt
     1441 tagcatctgg actgggagac tggattgcaa gagttatatc tccacttgca ggtaatattg
     1501 gtggattatt gattatatca gtaatttgca cacttccatt cttatcacca atactaggac
     1561 ctggtgcagt tatagctcaa gtagtaggga ctcttgtagg tacacaaatt ggtcttggtg
     1621 caataccagc atatttagca cttccagctt tatttgcaat aaatgggcaa gctggatgtg
     1681 attttgtacc agtaggatta agtttgggag aagcagaacc agaaactgta gagtatggtg
     1741 ttccagcttt attttactct cgtttaataa ctggtccaat agctgttatt attgcatatg
     1801 gagttgcagt atttgcattg agataatatt taatgtcaat ataaggcttg gaattaatta
     1861 ataaaattgt aaatataaat ttaagctagt caagattttt tgttaatgaa agactagctt
     1921 ttatactatt aattagctgt aattatcgag ttagattaat actaaatttt cctttattaa
     1981 agtgaaattt tatgtattaa tttggtttca aatttgtact agaatgaaat caaggaggag
     2041 atacaaaatg agtgtaactg agaaaattcc ttctattgac cgcaagatat ttaagatatt
     2101 gattatgtgt agtaaaaaag agtttgtgag tattaactca atagcaaatg aattgaatgt
     2161 cacaactaga agtgtaagga cgtatataaa acaattgaat aaagatttag gtaatgatat
     2221 agcagtaatc aaatatataa agggtcaagg ctataaacta gaaataaaag atgagcagat
     2281 actcaataag attattgata tgaatagaaa aaatgtattt tcattaaatt caaaagaaga
     2341 tagagtagag tttatactaa attacttgat agaactagat ggatttatta cacttgatag
     2401 tttagctgat gagatgtgtg taggaagaac tactttagta aatgatttcc agtatgtaga
     2461 aaaagttttg gcttcatata atttaaactt gattaaaaaa cagaatacag gtatgaaatt
     2521 aaatggaaat gaacttgata ttagactgtt tatattaaat cagttgtata aaaactcaag
     2581 gaaagatttc agtaattcaa agtattttaa aggaatcaaa aaagaagaaa tcataaattt
     2641 agaagaaaaa ctattgactt tatttaaaaa aaataatttt tatgttacag atgaaatgtt
     2701 aagagaagtt attaactaca tagtagtttt agtctataga gtaaaagaag aaaagaaagt
     2761 aaaagattat gatgtaaagt ttgatttact taaatcatat gatgaatact ttattgctag
     2821 ggaaataaaa catataatat caggaatgtt tgaatgtact ttaaatgatg aagaaattat
     2881 ttatttaact ataccgttag ttagtggaaa tgcaccagca tcagaatgtg ctttaaatag
     2941 tagtagaatt agtaaaaaca ttgatgagtt gatggaaaat atatttaatc aaatatatgt
     3001 cgatatgggt atttttataa atgaggatga attaagagta ggtctaggat atcatcttag
     3061 ttttacttta aacaggctat tatttaatat aaagttaaaa aatgttttgt tagaagagat
     3121 taagcaaaat tatatacttc catttaaatt agcacaaata gcaggaaaag taatagagaa
     3181 aaagtataac ttagaagttt cagaagatga gattggatat atagcaatcc atttcagtgg
     3241 atatttagaa agaaatatca gtagattcta ttcaataaaa aaaatagcaa ttatatgtag
     3301 tacaggtcta ggaacagcaa aactattgaa aataagagta gaaaaactga taggaaataa
     3361 tcctaaaatt gatactcttt caagttttaa tttgagaaat attaacttag atgaatacga
     3421 tatagttttt acaactattg atttagacac atcaaatata aatacaatag tattaaaaat
     3481 aaatacaata tttgatgaaa acaaactaag agaacaatta aaaacagtat tgtgcctaag
     3541 agaagggaat attgacacta ctaattctac aaatttacta ataaataatt tgttagatga
     3601 agataaattt atgatattaa atgaaaagac tatacttgct tcattagaaa aaatgatgga
     3661 ccatcttatg gatttgggat gcattgatga taagtttaga aaaaatatag tcaatagaga
     3721 agaaaaatct cccacagtat ttgataaagg tttattattt cctcattctg taaatgaaaa
     3781 atcagataag tttttaatgg cagttggaat tttagaagaa ccaatagaat atgcaaatag
     3841 aagtataaaa ataattctta tgacaatgtt tccatgtgag aataagatag actcagactt
     3901 attagtaaaa atttatgaag aagttttaaa gataggccaa gatgtaaaat taacgaataa
     3961 gataagtaaa tgtagaactt ttttagaatt taaaaaggtg ttacttaaaa accttatata
     4021 actattgtac agcatttata gttgttatac agatttaaaa ataatgtgat ttttggaagg
     4081 tgtataaata tgaaattatt agtgatttta tttgttgctt atattttaaa tactatattt
     4141 ataatgaagc agaataaatt gtattttaaa actttaaata gagctaaaaa aaagggtgat
     4201 atagtgtcta caggcaaaaa gaaaagctat ttttctaaag gaagtatagc cattatatct
     4261 tcagattcag atgggtttat aaagtttgga gaaattttaa aaggacgtac agtaatggct
     4321 aaatttaaag agattgaaga aatagaagga ttagatatat atagtgcaga gcaaaaattt
     4381 aaagatgaag agagtattgt acaagctata aaatttataa aagaaaaaat aaatgtttct
     4441 tttaattaac tcaatataaa tataaattat caggaggaat gtcatgaaat atgaagttaa
     4501 gataagtggg ataggcagtt tagttaaaga gttaatggat gaaagcaatt gtcttattat
     4561 atatgatgaa accattaatg atgacgactt aaaggatata tctgtaattc attcaatagc
     4621 aacgttaaaa agtgatgttg aaataggaga tacacttact atagggaata gagattattg
     4681 tatagtttca gttggtgata ttgctcagaa gactcttaga gaaattgggc attgtacaat
     4741 caaatttgat ggaaaatgtg aagtaaattt accaggagaa atacatgtgg aaataggaaa
     4801 tccagatata actattggag atttaataac tatttcttaa tttgaaaata aaattattat
     4861 aacaggagga attggtatgt tagatttaaa cagaaaactt gcaaaattgg aagaggaagg
     4921 taaaaaaata aaagtagctc ttataggagc tgggcatatg ggaaatggaa tggtaagcca
     4981 aatggcaaat atgaaaggta ttgaagcttc tattgttgta gatataaatt tggagttggc
     5041 tcataaagct tttactgatg caggaataga tgaagaaatt atagataatg taacaaatga
     5101 aaaggatgca gaaataaaat tacaagaagg aaaagtattg acatgtaatg attttcttgt
     5161 ggcttgtaaa actaagtcaa tagatgtagt tatagatgca actggaggta ttgcaattgg
     5221 tgcagagata gcattaaact ctattttaaa taaaaaacat atagttatgt taaatgctga
     5281 aactgattgt gttgttggac caatacttaa aaaattagca gatgatgctg gagtaatatt
     5341 tacaggttct gcaggagatg agccaggtgc agtaatggaa ttatttgatt ttgcagatgc
     5401 aatggggttt gaagttagag tgataggtaa aggtaaaaat aataaattag atttagattg
     5461 taatcctgat acagttagag aagaagcaga aagaaaagga gcttcacctc atatgatagc
     5521 ttcttttaaa gaaggaacta aaacaatggt agaaatggct ttaatgtgta atgcaactgg
     5581 atttgtgcct gatgtaagag gtggtcatgg tattgaagct acagttaatg aagtacctaa
     5641 gaaatatgca ttaaaaagcg aaggtggagt attagataat tacggtgtag ttgattttgt
     5701 aaatggtata gcacctggag ttttcgtagt agtagcccat aaattaaaag cagttaatga
     5761 tgaactaaaa tatttaagta tgggtgatgg acctaattac atattatata gaccatatca
     5821 tttatgtagt ttagagactc ctttatcagc agctatggca gttttagacc ataaggctac
     5881 aatagtacct aaagctggac tagtagcaga agttatgact atagctaaga aagatttgaa
     5941 aaaaggtgag tttatggatg gctatggagc atatacatgc tatggtacta tagaaaaata
     6001 cgatgtagca aaggctatga atgctgtacc tattggtttg ataagtaaaa agactaaagt
     6061 tgtaaaagat attaaaaaag gtgaagttat aacttacgat atggtagaga tagaaaaaga
     6121 tactacttta tatcatttaa gacaattgca agaaaaaata tttgggtaag atttgatggt
     6181 aatataaata gatattagta aaaagggttg atggttattt ttaaatcaac ccttttaaat
     6241 ttttatatat tcttgttagg atactttctt taaaagtata ttataggtta tttgtagtcg
     6301 taaaaattat tttgaatt
//
LOCUS       DAEMHW010000201         6305 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEMHW010000201 DAEMHW010000000
VERSION     DAEMHW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.201"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            357..515
                     /locus_tag="KPK67_003237"
     CDS             357..515
                     /locus_tag="KPK67_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460161.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(802..1506)
                     /locus_tag="KPK67_003238"
     CDS             complement(802..1506)
                     /locus_tag="KPK67_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2460162.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1497..3104)
                     /locus_tag="KPK67_003239"
     CDS             complement(1497..3104)
                     /locus_tag="KPK67_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBG2460163.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(3358..4536)
                     /locus_tag="KPK67_003240"
     CDS             complement(3358..4536)
                     /locus_tag="KPK67_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460164.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4762..5256)
                     /locus_tag="KPK67_003241"
     CDS             complement(4762..5256)
                     /locus_tag="KPK67_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG2460165.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5257..6018)
                     /locus_tag="KPK67_003242"
     CDS             complement(5257..6018)
                     /locus_tag="KPK67_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG2460166.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
BASE COUNT         2111 a         1168 c          693 g         2333 t
ORIGIN      
        1 attatttaga ttgttacttt gatatattat ataatatcac aaaggctgat ataaaaagac
       61 aaaaagggca atttttttat atcaaaaaaa atgttaaatt gatattatcc atataggtgc
      121 tatatttaac aattctactt acttcccttg cacttattag catatagtta ttaagaattt
      181 gttgcaaaac aacacttagc acactttaaa aagtgtggtc taataagtaa tcaacgaaga
      241 agacataata aagcataaaa taggatatac ttttccacgc catcaaaact gaggatttta
      301 ttattaatat atgctaaaat atgcttaata aattaaattt taatgggaga atagttatga
      361 aaatatttag tttcttgttg tttgtgatag gaatatgttt atgtattgct ggatttatag
      421 gacattaccc agtatttgtt ggtacaacat tgagttttct tgcaattgca atgatagctg
      481 taagacaagt caaaactaag aaaaaaatga aataggcaaa tcctaacttt acaagttaat
      541 attacacaag taattagtct aaaattggat actttttcta ttacagaaat acatcatcca
      601 ctttgtattt tcttaaaaaa ataaaatagc tatgtaaatt caagattaga tagattaact
      661 ctctatccat ttcattgtaa aatatacata gctatttgta ttcgatttaa aatacttaga
      721 ttttcttaca taacaaataa tttctatctg attattactc atattcattt agtaaccact
      781 cagcaactaa tcatttagta tctattcatc cgccatacgg taacctaccc caacctcagt
      841 caaaatatat tctggctgag caggattctt ttcaattttc cttcttatac ttgccataaa
      901 aactctaagc gactgatttt cactttcata gtaatttccc caaactttat ctattataaa
      961 cttatgggtt aagactcttc ctgaatactt agacatcaac tcaataatct tatattcgat
     1021 tggagttaaa tgtatttctt caccattaat tgacacaata tgattttcat aatctatctt
     1081 taggttttta acttcaaaag actttggagc atcattattt atattatcct gttgagcttt
     1141 atttctaaga gccacacgta ctctagccaa aagctctgtt acactaaaag gtttagttaa
     1201 ataatcgtct gctcctccat ctaaagcttc caccttatcc ctctcatgct ctctcgctga
     1261 aacaacaatt attttattag tctttgcaca tgccctaact cgtcctatta cctcaatccc
     1321 atcaatatct ggtagtccta aatccaaaat tatcaaatct gggtcaagag aatgtataag
     1381 tgatatagct gttcctccgt tatcggtctc tacacaccta taattttgag tttcaagaga
     1441 tacttttata aatttacaaa ttggcttatc atcttctaca attagaacta atggctttat
     1501 ttccacttgt ctttcccctc ctcatcatac ataggtatgc taaatctaaa tgtagctcct
     1561 ccagaaggat tattaaaggc gacaatttct ccaccatgag cttctactat agatttacat
     1621 atctccaatc caagaccaat acctcttctt tcaagatttc tagacttatt cttagtataa
     1681 aacctattaa aaatattccc gatatcttca tttggtattc cttttccatt atctgcaact
     1741 tcaaatagta cttgatttga ctcttttttt acagttagtt ctatcttaga attttttggt
     1801 gtatgcctta tagcgttatc aattagattt actaacactt gttctattaa taatacatcc
     1861 acatgaacga ttattatctc atctggaata tttgttttta tgtctctact attggcaaac
     1921 tttttgactc ttaatataga ttcagctatt atttcatcaa caacctctgg tcgtttttca
     1981 atatccaatt ttccttcatc tattctggtc atactaagta tattctcaac agaatgagtt
     2041 agccaactcg tatcttcata tatatttttc aataattcta ctctagtttc tttatctatc
     2101 acatcatcat tttcaagtat tgttgaagat gaacctaata tacttgtaag tggagttctt
     2161 aaatcatgag aaattgacct aagcaagttt cctcttaatc tctctctctc tgcctcaaga
     2221 tttgcttttt tatttttctc aaataaattt tctctatcaa ttgccaacgc aacctgtgtt
     2281 gaaactgctt ctaatagtat cttttcattt tctgtaataa tatctatctt aaaacacgca
     2341 acacctataa caccaagaac atttgattct tgacctttta tagggaaata atatgcactg
     2401 ctgtttttac aatattttgt cccaattcca actgggttac ctgtcctaaa agcttctttt
     2461 atagcttcct tttctaatac tgaatggaat atattctcac ttccatcatt gttacaaata
     2521 taaatacttg gcttacctag attattattc tcatctgcaa tagtcactat tacagttcta
     2581 tttaatatat ttactaaatt agacccacaa aattcaataa tttgattttt attttttgct
     2641 ttaagcaacg ttttgttatt ctcatagagc aatcttatca ttttttctct cttatgagaa
     2701 agtctcactt ctctttttat cctagaagtt aaagtacttg ttataattgc tgccattagc
     2761 ataatacaaa aggtcatagg ataatctttt ctataagcta aaaaagtata taatggatgc
     2821 gtaaagaaaa aattaaatga tagcactcct attactgaag aaagtattcc aaaaaaatat
     2881 ccataggtta tacttgtagc aaatagtaca cctaatatgt atactagtat aatattagat
     2941 tcattaaaac ctaccttttc aaaaactact gatattccag tagcaattgt cataaccaat
     3001 atagttttta tgaagtcctt aataattatc tttttactga gatagccatc ttcatcataa
     3061 tcatactctt ttaagtcttc atgttgtagt cgttcctttt tcatttacta caattcctcc
     3121 ttattgttac tgtttaaatt ggctctattt tatataatac aattttatca taataaattc
     3181 aatcaataca tttaattaat gtacttaaat tactaaaagg tgctgtctta aaatgaaaat
     3241 aaatctctat ttcaaaacag cacctatata tttaaattac taatttagtt tttacatact
     3301 taaatcaata gttttattca atttcataaa ttataatact tcaattataa aacatttcta
     3361 tccaacaagc ataaatataa agtgggcaca tatacctgca cataaaccac ctatagtatg
     3421 cgaaagtatc gctttaccag ccaatggtct tgcatctaaa gcatccatca tacctatgtg
     3481 tgtacttaaa tatccactcc aacacatacc cattgctgtg aatactgcta tatcattagg
     3541 agttatagcg cctgatttta taaactctgg tactaaagat attgctgctc caacagctcc
     3601 taatgcagtt acagggaatg ctattgcctc tggtgatgtg aatccaaata atggttctat
     3661 tatgaagcta atcttatcac caatagcagg taataatttt ataccttcat atgcagctcc
     3721 tgtgtaaaca gcttgacctg tagcagggtc tgtagatggt ccaaaagtta ataacataac
     3781 taatgtacaa actactaaaa ctcctggtat tatagccata cccatttcaa cacctaactt
     3841 accaccttca agtatagcat ccaatgttct ttggaataca ttaccatctc ttatttctct
     3901 aaactcttca agttttgcac ctttatcatt tatttgcttt tcagtatcat ttttagggtc
     3961 ataattataa tactttttag taaaagtcaa cataattcta acactaataa tactacctat
     4021 tattgccgca acattaccta ttatagctgg tagtacatat tctttacctt gagctatcat
     4081 aaaagttgtt acaataagtc ccataccaaa ggctgttcct aaattacata aagcaggtac
     4141 ttgatacttc ttaaaatatt gtgtaaatgt tttgtcttta gcaaatggta taatagctgg
     4201 attatcagat aaatatgttg caactactcc tgctatacta gcaccaggta aaccccatat
     4261 aggtctcata agacccttaa atatcttatt aactaaagat attactccaa actctgaaag
     4321 caatgcacta agtgcacctg caagaactgc catagccatt attaaaaata ctgtgtctaa
     4381 taaaagcgca tgtgctgtac tcattattac tttaaacatc attcctgcac ccatgatgct
     4441 tcctacatat ccaaatccta ccgccaatag tacaataaat acgaaggttt ccatacttac
     4501 tgccttgact cttctcttat ccgacagctt ttccatcttg aaatcttccc ccttttaatt
     4561 ttaaattaag gttcctaaca ttggtacaaa ttttttggta caaactgagt tctaaaactt
     4621 acactcgtaa tgcaagttgt cagttttttg aaataaaaaa aaattacgtt tatagaataa
     4681 gaatatcctc tttaatttgg atactcttat cctattttat gttattatat tatcatcctt
     4741 ttgtttaaaa gtcaattttt tctatttttg ttgtttaatt ttatttatac tctttttata
     4801 tttttcattt tcttttatct ttgtaagtac tccatataag ttgacttttc taaatacagt
     4861 agttataaat accaatccta atgcaattga acttgcacac gataatgtac tcaccccata
     4921 ttctacagct tttatataat tattggtcaa aacagaatac atagtattat atattccata
     4981 acctggtact aatattatta aacagcaaac acttaatgta gttgctggag tcatataaat
     5041 tcttgcacat aactcacaat atatcgcaaa acaaactgta gctataaaaa aacataagtt
     5101 ttctgttact cccatattta aaagaaattt atatgaaaac caagctatac ttccacctat
     5161 tcctgctagt attaagtttt ttcctttcat attaaatagc actccaaagc ttaaggcagt
     5221 aaaaaaagct gctattactt ctataaacaa atgcacttat atccccccta acttgaagta
     5281 caatccaagt accaagaaca ttcctactgc tatagataca cctacaaaaa atacttctgc
     5341 taattttgca agaccagaaa tcaactgtcc atctaataaa tctcttaatg cattagtcaa
     5401 agcaagcccg ggaactaaga gcattattgc tcctgctatt aaatggtcta acgtctttat
     5461 gaatccgagc tttaaaatta aaattgaaca agaagctata ataactgctc caattgcatt
     5521 tacaaaaaag tcattaaggg aagatttact taaatacata ataacaaact tagtcagaat
     5581 cccaacagta aatgcagcta taaattcttc cacatttcct ccaaataata tcgaaaaagt
     5641 tgctgctgca atacctgcaa aaaatattgt tattaaatcc gagtaatagt tatcttcact
     5701 tatttcatct aaaattttct cacataattc aatatctaaa ttatttatac ttattgctct
     5761 tgataatgag ttaacttcat gaaccaagtt taggtctact cttcttgagc ttattctttt
     5821 aattgctgaa tgtaatttcc catctacaaa tacagatgcc attacagctg ttggagttgc
     5881 aaatacatct acgtggtcta ttccaaaaga ttgacatatt ctagctatcg tttcttcaac
     5941 tctataggtt tctcctccac tttgcaacat agctttacct gcatttgagg aaaagtttaa
     6001 aattctatct atatccacaa aacatatctc ccattctttt atagtattat tcatatttta
     6061 atacaagata ttatttattt aaactatttt aaataattca tatccaatat ttacatttta
     6121 tgaaaaaact tgttttttat gcttgtcaaa atcaaatttt aagcttgttt acaccataaa
     6181 atggctgaat taatatttga caaacacttt caaccattta tataaccttc taaattgtat
     6241 aactttttac tacaaataaa attatatctc ttttaattct ttctcttgag tatttagctt
     6301 atagt
//
LOCUS       DAEMHW010000202         6136 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEMHW010000202 DAEMHW010000000
VERSION     DAEMHW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.202"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            56..706
                     /locus_tag="KPK67_003243"
     CDS             56..706
                     /locus_tag="KPK67_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBG2460167.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(846..2222)
                     /locus_tag="KPK67_003244"
     CDS             complement(846..2222)
                     /locus_tag="KPK67_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2460168.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2238..4151)
                     /locus_tag="KPK67_003245"
     CDS             complement(2238..4151)
                     /locus_tag="KPK67_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2460169.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVRISEESTKPNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4287..4919
                     /locus_tag="KPK67_003246"
     CDS             4287..4919
                     /locus_tag="KPK67_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBG2460170.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5058..5597
                     /locus_tag="KPK67_003247"
     CDS             5058..5597
                     /locus_tag="KPK67_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG2460171.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAARKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(5773..5943)
                     /locus_tag="KPK67_003248"
     CDS             complement(5773..5943)
                     /locus_tag="KPK67_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG2460172.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
BASE COUNT         2270 a          857 c          856 g         2153 t
ORIGIN      
        1 ttaaaacata taaataaaat attttataca atatattgta aaggaggtaa ctttgatgaa
       61 agcagaaaaa acaagtatat atccaattat taaaaataat ttaaacaaaa tatcaattgt
      121 tgaatataaa aagggtcaaa gatttgtagc atcagataaa gacttacaag aagtgttctt
      181 tataatagag ggagttgcat tagtggagtg tactactaga ggaggaaata aatttcttgt
      241 tgacattgta cctgaaaatg aatttatagg caaaattagt tacatatatg aacataattt
      301 gaaatgtgat atatttgcaa agactaatat aaaattattt cgatttgaaa aaaatgtatt
      361 tgaagagttt tatctcaaac cagattttat tgcactattt cacagaaaat gtacaagaag
      421 aatatatgaa ttatataaaa ccagaatggt aagagaacta ttttcttgca cagaagttat
      481 tgcctattgt atatcaaata aacaagaaaa taatgtctgc aatataaaag ctattcgtaa
      541 tttcagtgaa atctattcaa ttagtagaaa aagtagatac cattctctgc taaagttaac
      601 tgaaaaggag atagtaaaaa aaatgggtaa tacatatgaa attttaaact atgatgaatt
      661 acataaattg gcatttgaag taaaagaatt tttggaagat gagtaaaaat tgttacttaa
      721 tcaaaataaa gtataataat ttctgtaaaa aatagaaata gtctcactga aaatcaaaaa
      781 tactttagtt attttcaatg agactgttta gaaatattgt atacttataa tgtatagtat
      841 aattattaat caatttcctt tactatatta atagcccatt ttttagataa tgctctagga
      901 acaccaaata taaacttaac cacttcttca ctataagtga gcaaaacgag tacaaatata
      961 ggtgcacctt taaatgcagc tataaaagtt agaggaatag aagctaacca catacatcct
     1021 aattctagaa atagagcata tttagtatct cctccacttc ttaaaatacc aattataacc
     1081 aatgtattaa aagatttaag agccattagt attcccatta taaagaatat cttagttata
     1141 tcagaagcta agctatcaga aacactaaac atttttaata ataatggtat atttaaaatt
     1201 aaaataattc caagtattaa accagctgaa aatacaagta tagaaaactt tttagcatat
     1261 tcaatggctc ttttaatatg gtcagctcca agttcatttc caagcataat agatgcacca
     1321 gatgcaatac atacagctgt cattatgaag aaattacctg tactggttgc tatctggcta
     1381 gctgctatag cagaagtacc agctcttgca tatgcaacag aatacaggac agttccaatt
     1441 gcccatacag agtcatttac aaatatagga aagctttttg atgaaaaaga ttttataaaa
     1501 attttatcaa tagcttttag attttttaga ccaaacttta atatatagtc ttttttataa
     1561 aggtaaacat aagtaatcat taaaattaat tcacatattc tagcaataac agtagctaga
     1621 gcagcacctt ttacacccaa tgcaggaaga ccgaagttac caaatataaa tccataattt
     1681 aaaataacat ttgttacaag tgcaaatgca ctacaaatca tacctaactt aggatttcta
     1741 acacctcttg aaccagtact aaatacagta cttacagcaa ttaatggata actaaatact
     1801 gcaatactaa agtaatctat acaaagtttt accacctcag aatcatacga aaatatgtgt
     1861 ataattggct ttggtgttag taaagcagga ataacaaata caaaacttaa aacaattgcc
     1921 aatacacaag taagacctgt gacttttctt atattactca catccttctt tccataaaac
     1981 tgtgaaataa aaactccagc acctcctgtg ataccagata aggacatatt aaaaagaaag
     2041 aaaaattggt ttgcaactcc aactgaagct acagatgttt cccccaaact acttatcata
     2101 attgtatcta ttacatttac agaagtagaa attaagttct gtatgataat aggtatacat
     2161 aaagatagaa gtattttata aaatctttta ttttcgtctt ttaaagaaaa tagttcttgc
     2221 aaataatcct ctccttttta cataaattct tcccactcat catatagaga ggcaaggtta
     2281 ttttctaaat taattttttc ttggctaact tctttagctt tatctggatt agagtatact
     2341 tcttcttgac ataaaagtat atctaatttt tcaatttcaa tctctaactt ttcaatttct
     2401 ttttctatat tttgtctttt tattctattt ttcttttcta tttctctttg ttctctctct
     2461 tttctttttt catcttttat ttgagttcta gttttttctt ctttttcttc tataatactc
     2521 atctcttgga tttgcctttt tttatctata taataatcat aatttcccag atattcagta
     2581 attccatttt catctaaaac taaaatttta tcaacaacag tatttagaaa gtatctatca
     2641 tgtgatattg taaatatagt cccatcataa ttagttaatg cctcttcaag gacttcctta
     2701 gaatcaatat ctaggtgatt agtaggctca tctaataaca ataagtttgc atttgatagt
     2761 ataagtttaa gtatggcaac tctagctctt tctccaccac tcaatgtaga aatcttctta
     2821 aaaacttctt catctacaaa taaaaatgcc cctaacatat ttctcaaagt agtttgagta
     2881 agatgagtat tattttccca aatctcatca attattgtgt tatctaaatt aagagttttt
     2941 tgttcctgat ggaagtagga aacatgtaca ttagttccaa atttaatatt gccactaaga
     3001 ggttgtagtt cattagttat tattttaaat aatgtagatt tgccaacacc atttgcacct
     3061 ataagtgcaa ctttttcccc tctatatata tctaaatcca agtctttaaa aagtattctt
     3121 tctccataac ccatagatac atctctaatt tgaagaacat cattaccact ctggacagaa
     3181 ggcgtaaatt gtatacgagc ttttttccta taagcttctg gtttatctaa aacgtctacc
     3241 ttgtctaatg ctttttcttt acttcttgca cgctttaggt gtttttctct accataagct
     3301 tttagtcttt caattgattc ttcttgcttt tttatttctt tttgttggtc ttcaaatttc
     3361 ttaagctcta gttctttttc tattttagat aactcaacaa atttagagta gttaccatta
     3421 tatactttaa gctttttgtt gtgaacttca aaaattctat ttacggattg gtctaagaaa
     3481 tatctatcat gagatattaa catgacagtc cctttatatt gttttaaaaa tacttctaac
     3541 cattctattg cttcagagtc taagtggtta gtaggttcat caagtaacaa tagagttggt
     3601 ttcttaagaa gtaattttcc taaaagaact ctagtttttt caccaccaga aagtatgctt
     3661 attggcttat ccatatcatt gtcactaaat cctaatcctt ttagaactcc tttggcttca
     3721 gatttatatc catatccatt taaatctgaa aataattcta gtttatgtga atattcatcc
     3781 atgatttttt ctataattgg agagtttggt tttgtacttt cttcagatat tctaacctcc
     3841 aattctctta aataggattc catttctatc aaatttttaa aaacttctag tacttcttcc
     3901 aaaatagtat tatccgaata aaaattggta ttttgttcta aatatccgat ttcacaatct
     3961 ttagaagtgt aaatatctcc actatcatat ccataaattc ctgaaattat tttaaataaa
     4021 gtagtttttc cagtaccatt aacaccgatt ataccaattt tatcgccttc atttactgta
     4081 aaactaatat tttctaatat agaatctata ccaaaacttt tatttaagtt attacatgac
     4141 aaaacaatca taaatttcta cctccttgaa ctatctaaaa ttataatgtt ttgatataat
     4201 ataatcaagg ttatatgtat tagttttgaa aaagaaatat tttattgtga atcaaacaaa
     4261 aaagaggaca cttttaggag gtgaagatgt tgggaaataa aaatatatca atggcagtta
     4321 taagaaggct cccaaaatat catagatatc ttggagactt attagatagg gatatacaaa
     4381 gaatatcttc taaagaattg agtgatataa tagggtttac cgcttctcaa ataagacaag
     4441 atttaaacaa ctttggtgga tttggacaac aaggatatgg ttataatgta gaagctcttc
     4501 atactgagat aggtaaaatt cttgggttgg atcgaccata caacgcagtt cttgtaggag
     4561 caggtaactt aggacaagct atagccaatt atgcaggatt tagaaaagct ggattcgaga
     4621 taaaagcttt atttgatgca aatcctagaa tgataggttt aaagataaga gagtttgaag
     4681 tattagattc agatacttta gaagacttta taaaaaacaa taatatagat attgctgtat
     4741 tatgtatacc taaaaatgga gcacaagaag ttattaatag agttgtaaaa gctggaatca
     4801 aaggtgtatg gaattttgca cctttagatt tagaagttcc gaaaggtgtt atagttgaaa
     4861 atgtaaactt aacagaaagt ttatttacct tatcgtattt aatgaaagaa ggaaagtaga
     4921 caacttaaac atattaataa tgagcagttg tgaagagtat tgagaaagtc agaagattta
     4981 atattaaatc ctctggcttt ttgaaatatt aggataaaaa tttaaataat gtatggaaat
     5041 aaacaatatt aaggagagtg gtttttgtgg aggaattatg gaaaaaggca attgaatttc
     5101 atggtcatga atgtccagga ttagctatag gatttaaagc agcacttgca gcaagaaaat
     5161 atttaggatg taaacagtca ggagatgagg aaattgtatg tatttctgaa aatgatgctt
     5221 gtggaataga tggtatacaa gtcgtgctaa gttgcacaat gggtaaagga aatttactac
     5281 ttaggatgac tgggaaatca gtatatcatt tctttaatag ggaaaatgga aaatctataa
     5341 gaatatatgt aaagtcaaag ccatttcaaa tggatagaga aggatacaaa aagtacttat
     5401 tagaaagtga ttttgaagaa atatttgaga ttacagatac taagttaaga cttcctgaaa
     5461 aagcaagaat atttaaatct cttaaatgtg aagtgtgtgg tgaatttgca tctgagcaat
     5521 atataaggat tgaagaagga aagaaagtat gtcttgattg ctttaataaa tatgaaagat
     5581 tttatgagga gtattaaaat tataaattag attaaagtta aaagtattag atatgtgcta
     5641 acagtatttt aagatgaatt aggtagcaac ataataaagt atataaataa tattaattga
     5701 atcatacata attaaaaaga ggctgattag tatttataaa ataaaatcag tctctttatg
     5761 aataacgaat tattattctg gttgaggtgc atctacagga caagctcctg cacaagaacc
     5821 acagtctata caagaaccag catctataac atatgcgtcg tctccagctg atatacagct
     5881 aacagggcat tcagcctcac aagcaccaca gcttatacaa gcgtcagtaa ttttatatgc
     5941 cattttgaaa tcctcccttt tatatgtatt gtaattcaat aacattataa caaatattat
     6001 tgaatataac aattaaatta attctacaac aaaaacttta ttatgatatt cttgttttaa
     6061 agttttacaa taaagtttaa tctgcttata tttttattta ttttaaagaa gtttatttta
     6121 aaaataaaat taaatt
//
LOCUS       DAEMHW010000203         6029 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEMHW010000203 DAEMHW010000000
VERSION     DAEMHW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.203"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            261..986
                     /locus_tag="KPK67_003249"
     CDS             261..986
                     /locus_tag="KPK67_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG2460173.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            complement(1114..2310)
                     /locus_tag="KPK67_003250"
     CDS             complement(1114..2310)
                     /locus_tag="KPK67_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460174.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(2313..3338)
                     /locus_tag="KPK67_003251"
     CDS             complement(2313..3338)
                     /locus_tag="KPK67_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG2460175.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KLIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            complement(3410..4540)
                     /locus_tag="KPK67_003252"
     CDS             complement(3410..4540)
                     /locus_tag="KPK67_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2460176.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            complement(4782..4922)
                     /locus_tag="KPK67_003253"
     CDS             complement(4782..4922)
                     /locus_tag="KPK67_003253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460177.1"
                     /translation="MIRIKQHRQFDRVVLYLKHHQINVIFLLVCQRSDSDANRKCWEL
                     SY"
     gene            complement(5134..5529)
                     /gene="tnpA"
                     /locus_tag="KPK67_003254"
     CDS             complement(5134..5529)
                     /gene="tnpA"
                     /locus_tag="KPK67_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG2460178.1"
                     /translation="MKLDSNNHSVFLLHYHLVMCIKYRRKVIDDSISDRLKDIFVDIA
                     PNYGVVLIEWNHYVDHVHLLFKAKPNTEISKLLNAYKSASSRRIKNEFPSIRKYLWKE
                     YFWSKSYCLVTTGGATLEDLEQYIENQEE"
     gene            complement(5694..>6029)
                     /locus_tag="KPK67_003255"
     CDS             complement(5694..>6029)
                     /locus_tag="KPK67_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2460179.1"
                     /translation="VEFILNQIVVNSIKYIKSKDGLIKISAKRVENSIVLTIEDNGVG
                     IVERDINKVFEKGFTGENGRVFGKSTGIGLYLCKKLCNKLGLGLILESKVNVGTVIKV
                     SFPIDYNLF"
BASE COUNT         2034 a         1026 c          631 g         2338 t
ORIGIN      
        1 ttactttata attgacatta tttttattta aagaaatttt tatcaactca aatataaaac
       61 aatataagct tattatgtcc tgtttaaaaa tttaaattat ctctaaatag cttaaaaata
      121 tattctgata tattgaaaaa agtactttca agatgatatt ttatatatta tttagaagtt
      181 ttttatttat tgttatataa ttaaatatat gactattttg ctactacata tataaaaata
      241 aatttatgaa ggtgatttta atgaaaacta tttctaaaca agaattagaa aaaacacttg
      301 agaaacatta tttatggctt aaaaacaata atgctggaga aaaggcagat ttaaaagaat
      361 taagattaga aaatatggac ttaagaggat actgcttaag taatattgac ttttcgtggt
      421 ctgaccttat caatttaaat ttagaaaaag cagatttaga aaactctatt tttaataatt
      481 cttatctttc ggattgttca ttaaaaaatt ctatattaaa aaatgcagac ttaagtggtg
      541 caagttttag attttgtgac ttatctaact cagatattag aggagctaat ttagaaaatt
      601 caaatttaga acatgctact ttaaatggag taatatcaga tgaaagtacc cgttttttta
      661 aattgtattg tcctgaaaca ggagcattta taggctataa aaaatgtttt aattttagaa
      721 tggtacaatt attaataccc agcgacgcaa aaagagtgtc tgctacatca aatgcttgta
      781 gatgtgataa ggcaaaagta ctgtcaatta ctagtataga tggtaaagaa tcttttaaaa
      841 gtgctcgtgc atatgctgat gaaaatttta tatatagagt aggagaaacg attgtagctg
      901 aagattttaa tgaaaataga tggaaagaat cgactacagg gattcacttt tttttaacta
      961 gagaggaagc tattggttat ctataaaatg ctaaagtttt ataaatatat aaatacataa
     1021 acaaaataac aaaagagtat acattgtaat ttcaaaaaaa ttataatcta tactcttttt
     1081 tctatagcac ataagtacat tttatttcat aaattataat aaattattta tatctgcact
     1141 attgattatt tgagaaactt tatctatatg ttctatctta ctatccaaac tgtcataata
     1201 cttcttactc tcatctctat ttctaaaaga ataaactttt ttatccaatt ttaaagcctc
     1261 ttttataata tctatattcc tcttatttgt ctctcctaca ctaaatcctg tatctattat
     1321 aatcttggaa tcatttaaat ttctaatagc caaatcgaaa ctctcatcac ttataggctc
     1381 aaatggattt tctgtaaaca tctttatccc catagttcta ccaatttcat aatcaatatc
     1441 attttcatgt attattccag aatacagacc tatacctttt ttagtaaaag ctctataaat
     1501 tggtgttgct ttacccccac caccaattat aaaaacttca tttttagatt tattactaat
     1561 ttctactgaa cctaaaagac tattaaagtt tttatcatcc aattcataaa gcgaatttat
     1621 aatatcctca tctactacat cctcaggctg accataagct atgactttat tattttttat
     1681 aagagcaact ttatcacaac tttttaatgc tatgtctatt tcatgcaatg aaagtattac
     1741 tgatattgac ttttctttac ttaatttttt cagtatgttt atgagttcca atctatgttt
     1801 tatatccaag tgagttgtag gctcatctaa tataattatt tctggttctt gaacaagagc
     1861 ccttgcaacc aacacctttt gtttttctcc atcacttagt tcatcaaaat atcttttctt
     1921 taaatgtaag gcatctacag actccaatgc ttcatctacc attattaaat ctttcttaga
     1981 tagactccca aaaaatcctg tatatggata ccttcctata ttgactatat cttgtacagt
     2041 cattaaatca cctaataatc tatttgtaag tactaaagcc attttttttg ataatgcttt
     2101 tcttgatata caatttatgt ttacaccatt caaatatatt tcacctttta ttggtgatat
     2161 aagtttagat aaggacctta atagggtagt ttttccagct ccattacttc ccaaaagaca
     2221 tagtatttct ccatttttta cttctacatt tatattactt ataacaactt tgttattgta
     2281 tcctacactt aaattattag ttttcaacat gtctaagccc tatccttcct tttcaccaat
     2341 aacattacta caattggagc tcctaataaa gatgttatcg tacttattgg taattctact
     2401 ggtgataaaa ttactcttga tattaaatca caaagagctg taactatact gcctaaaaat
     2461 attactactg gtatcaaaac tctattatct gatgttttaa atatcaattt tgaaatttgt
     2521 ggcactgcaa gtccaataaa ccctataggt cctgcaaatg ctgatactat tgctgctaat
     2581 atactagata taaatactat caaaactcta aaaaatctta tatttatgcc aatacttttt
     2641 gcatactctt ctcctagtaa aaaagcattt aaaggtttta ttatgaatac agtcatcaaa
     2701 agacctaata taccaactat tataagtatt tctacctttt cccatgtaaa tccagaaaaa
     2761 cttcccatag accacatagt aaattctttt aatttatcac tatttgcaaa tgattgtaac
     2821 acatttgtta taccactaca tacatatccg gtcataattc ctattattaa aagagttgtt
     2881 atattcttta ccttatttgc aaataaaatg actatagaca ttacaattaa agctcctata
     2941 aaagctgcta caaatatact cattgaattt acactctcaa atcccaatgt gtatcctccc
     3001 aaaatcatga gagctactga taaagtagaa cctgaagata ctcctaatat atacggttca
     3061 actattgggt ttttaaaaaa tatctgtaat aaaatacctg atactgctag gcatgctcca
     3121 cctaccatgg ttgcaagtac tctagggatt cttatattgt atattatttt ataatgttct
     3181 gtattatcca tacctcttaa tacaacatct attacttctt taaattcaat ttttacagag
     3241 ccaaatgcta cacacactaa aaaagtaagt atcatactta taattaatac tataaatatt
     3301 gttgtattcc ttattctcat tttattgata aaatccattt gtatactcct cttagcaaag
     3361 ctatattaac taaaatctat aaaacctatc tttttgtgtc ctctctgtac tatttatcat
     3421 aatttatata atgtttgatt ttttcacctg tagtacctgg gtggaacatc tcaactaaat
     3481 ctactattaa ttcgtccgtc ttatctactg attgataata atctggagca aacacccata
     3541 ggtttccgtt ttttacaaca tccaaatcag ccaatacagg tgcactagat tttatagaat
     3601 ctaaatttgg tgcataatct gaacttgatg agtatacaaa tatatctgca tcttttgcaa
     3661 cttcatgcaa ttgttcaata cttactcgcc cttcatttga atctattgaa gcacatgcat
     3721 ttattccacc agccattttt atcatattat ccacatatga atctttagga gctatataag
     3781 cacttccttc atatacagat gcccatacaa ctttaggttt agctttgttt tttatttttt
     3841 tacttacttc ttctaccttt tgtacagttt tatttagttc actagtagcc atatcttctt
     3901 tatcataaaa tgcagctatt aaacttgtcc actccattct tccaaatgga ttttgctctt
     3961 tatactcatt gtctacaaca taattaagcc ctaattcttt taatttgtca ataaattttg
     4021 tatcagcctc tgataaacca ctactcacta tagttaagtc aggatttaag tctactattt
     4081 tttcataatc tggtgctaaa tttgaaccaa cgtaatgtat ttttccatct ttcattaaat
     4141 ctttaatttc tttaatagtc cagtactgct cttctgttgt tacggctttt atactatcta
     4201 gttcatctat tgttctcaga gaacaagcct gtgttgtaga tgctattaat acattgtcca
     4261 caggcgttct aactatatta gcatttttat atttttcagg aattttaatc ttctttggga
     4321 ttaaaatcag ttctcgatta tctccatcag ttactttttt aattccatct tctaaatctt
     4381 caacactaaa tccactagca tattttaatt ctaatttctt accagtacta gcttgtttat
     4441 tctctttgtc acttttacta caccctataa gacctcctac cataaaaatt accatagcaa
     4501 ttaaaaaaaa ctgctttaaa acatttgatt taaatttcat ctctttcact ccaatatctt
     4561 attttttatg agtggctaaa aaactttaat aagatatacc ttttaaagta tatcttatta
     4621 aagttatatt aaaccatctt taacaagtat aactttttaa taaaattata gtgaattttg
     4681 atttcacatg caattaacta cttatacact taactaccta cgacaacgtt tgaagttgca
     4741 tcttcttggt caaaatagct attgttatta gtatctccaa gctaatagga tagttcccaa
     4801 cacttacgat tagcatctga gtcactacgt tggcaaacta atagaaatat aacatttatt
     4861 tgatgatgct ttagatacag tacaactcta tcaaattgcc tatgttgttt tatcctaatc
     4921 attgatttta atttaagcag ttttatatac ccatttctat atgcacaata tcttttgagt
     4981 aacgtgttga caaactattt tggttagtag tatagctatg atagttaatt ttctttgata
     5041 gataagtccg ttacatccaa aatttttagg aaaagattcc ttttattcac tgtttgtata
     5101 aacctgtact tataagctct taacatatta tcactattct tcttgatttt ctatgtattg
     5161 ttccaaatct tccaatgttg caccaccagt tgttactaaa caataacttt tactccaaaa
     5221 gtattctttc cataagtatt tgcggatact tggaaactca ttctttatcc ttctgcttga
     5281 tgcagattta taggcattta ataacttgga aatttctgta ttaggctttg ctttgaataa
     5341 taagtgaaca tggtcaacat aatgattcca ttctatcaac accactccat aatttggtgc
     5401 aatatctaca aatatatctt ttagtctatc agatatagaa tcatctatta cttttcttct
     5461 atattttata cacataacta aatgataatg caataagaag actgaatgat tattactatc
     5521 taatttcatt ataattactt cccttcagct tttactacta aattaatgat ataatacgtt
     5581 tttatgaaaa tcaagtaaaa aaaagaacct atcaattatg cttacatcct taactttctt
     5641 aaataaatac aaaaaactga caactaaaaa ttgtcagttt ttataactat acactaaaat
     5701 aaattgtaat ctatagggaa tgataccttt atgactgtac caacatttac ttttgattct
     5761 aatattaaac ctaaacctaa cttattacat aactttttac atagatataa tcctattcca
     5821 gtactttttc caaatactct tccattctct cctgtaaacc ctttttcaaa taccttatta
     5881 atatctctct ctactatacc tactccatta tcttctatag ttaacactat tgagttttct
     5941 accctttttg cacttatttt tattaaacca tcttttgact ttatatattt tatagaattt
     6001 accacaattt gatttaatat aaactcaac
//
LOCUS       DAEMHW010000204         6027 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEMHW010000204 DAEMHW010000000
VERSION     DAEMHW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.204"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            188..1069
                     /locus_tag="KPK67_003256"
     CDS             188..1069
                     /locus_tag="KPK67_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBG2460180.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALFDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1451..2254
                     /locus_tag="KPK67_003257"
     CDS             1451..2254
                     /locus_tag="KPK67_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460181.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYANNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYSAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            2266..3396
                     /locus_tag="KPK67_003258"
     CDS             2266..3396
                     /locus_tag="KPK67_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2460182.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISRSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSKPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            3400..4809
                     /locus_tag="KPK67_003259"
     CDS             3400..4809
                     /locus_tag="KPK67_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460183.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSNGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQSI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            5020..5529
                     /locus_tag="KPK67_003260"
     CDS             5020..5529
                     /locus_tag="KPK67_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG2460184.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         2454 a          539 c         1067 g         1967 t
ORIGIN      
        1 ataaaattat atagttaata taattttata attggtttag tatagagatt tttaactttt
       61 atgttatact atttataaaa gtaggtataa agattaaaat acataatcaa atatgtataa
      121 gaggttttaa atagtattgt taatgaactt gattattgtt tataattaca aattggagga
      181 gataaacatg aaaattactg aaggatatat gccttttaaa ggttttaaaa cgtattaccg
      241 tatagtagga gaaaatacgg aagggaagaa gccattagta ttacttcatg gaggtccagg
      301 ttctacacac aactattttg aggtattgga taagatagct gaaagtggta gacaagtaat
      361 aatgtatgac caaattggtt gtggaaattc atttgtagaa gggcatcctg agctatttaa
      421 tgcagatact tggatagagg agcttattga actaagaaaa catctaggac ttgatgaaat
      481 tcatctgtta ggtcaatctt ggggaggtat gcaggctatt tggtatgcta ttgaatataa
      541 accaaaaggt attaagtctt acatcctttc atctactctt tcttctgcaa aactttggga
      601 gaaagaacaa aaaagaagaa tatcatatat gagtgaggtt gaccaaaaag cattatttga
      661 tgcagtaaat actggagatt actctagcaa agaatataat gatgcattag aaagatttat
      721 ggaaatgtat tgtgcaggtg aagtgacaga agattctcca gagtgcctga gaagacctaa
      781 aaaatcaggt tctgaagcat atattgtagg atggggacaa aatgaatttt ctcctacagg
      841 aactctttct ggatatgaat ttacagatag attacatgaa ataaaagagc cttgtctagt
      901 aactagtgga gcaatagatt tatgttcacc atatattgca aagactatgt atgatagaat
      961 acctaatagt aagtgggaat tatttgagta ttcaagacat atgccattcg ttgaagaaaa
     1021 tgagaaatat ataaaagtct taactgagtg gttgaatgcg aacgattaaa aactatataa
     1081 acttatataa aaaaatgagt gtctcaaaat gaacttttta gttcattagg cactcttttt
     1141 gcatgaaatc aaatatattt tcattatttg aacgataaaa tgaggcttat atctactttg
     1201 ggaaagtaat tttttattac tagcgttaga ttctaatcta attgtatacg tattgaaata
     1261 atagaaatat attggtgttt ataacttaat tttattgttt tttagaaatt tttttatata
     1321 ttggtataat tattctgttt aaatttgatt aaaaagtata ttaattataa taagaaacaa
     1381 ttatataaat aggaatagat tattataatt aatatagata taaaaataat tatagaaaga
     1441 aaggcacatt atggaaaaat tgagagttat tgaaatgata aaaaataatc ccaatattat
     1501 agctacaata gataatccaa ctgatgaaat gaaattgtta gctattaaag aaaatgggct
     1561 tgtactagaa tatataaata atccaaccag agagatgcaa gaactagcaa tagataataa
     1621 tattagagca attaaattta tagataatcc aacagaagat atgatgataa aagcagtaaa
     1681 tgagggatgg agtatactgg attatatcaa gaacccaact gataaagtta ttgaaatggc
     1741 tataaatcaa gcaggatggg caattaaata tgctaacaat ccaagtgaag aattacaact
     1801 attagcagta agaaaaaatt atgattcgat aaagtttata aaacaacctt atgaaagtgt
     1861 acaagaggag gcagtgagaa taagttatga tgccttgaga tatatagatt cacctagtta
     1921 tagtgcagaa cttatagcta taaaaaataa tgaagcagct attagcttta taactgattt
     1981 agataaaaat aaaatgttgg aatttttaaa agtgaatatt ttagtgatta agtacattct
     2041 gaaaaaagta tctaaagatg aattagagag tgtattaaaa gaagtattat ccaaagaaga
     2101 tgttgaggaa aagtatatta gagatttttt gaattgtagt gttatagata gaaatagtga
     2161 aagtatacca atagataaaa ttatgtttat ttataaatat ggaagtaaga cagcaaaaaa
     2221 aatagctgta gatgagaaat taaagattat gtaggagaga gaaaaatgaa ttttatagac
     2281 acattaaaaa gcgttgactt aatattatcc actggagaag tacctttaat agtaggtgaa
     2341 agtggaatag gaaaaacagc tttagcaaat aagcttgcca aagaaaatga ttggagttta
     2401 attgttattg atggaaactt acttaaagag ggtgaaatag gtggtcttcc aactatagaa
     2461 tcttatgtag gagttaattc taatggatat aagactgaaa agaaaacaac agtgtatgct
     2521 gttcataaca aattaagaga aattgatgaa gagatatcta gaagtaagac agttctttta
     2581 tttatagatg agataaatcg ttgtgagcat acagtacagc aagaacttat gaatttaatt
     2641 ttaaatagag aaattaatgg atataaactg catgatgatg taaagatttt agcagcaatg
     2701 aatccatcaa gtaaatatgg ttcagatttt gattatcaag tggtagatat ggatgcagcc
     2761 caagaaaata ggtttgtttg gttaaatatg gagagtgacc atacacaatg gataaaatgg
     2821 gcaatagatg agggtattga aagaaaagtt atcgagttta tttccacttt tccagaatat
     2881 ctgcataaaa taaatgaaga tgatgtaaga gctactccta gaagttatga gagagtatct
     2941 aaaatttata aagtttataa agaaaaaaat aattctattc ctagagcagt atttttaaat
     3001 gttgtaaagg gtaatgtagg taaagttatt gcagaagaat ttataagttt tattgaatca
     3061 aactctaaac cattaatatc ctatgaagat gtttttttag gagaatccat tgatgagtcc
     3121 attgtagaaa gagtaaaaaa tgagagtcat acaagacttt atctatcagc tatgaacatt
     3181 ctaaaagact tagaattaaa tattaagaat gataaatatg aatcaaacca ctatattaat
     3241 agatttattg aatttttaaa aatgtatcct gtagatttaa tgataggtat tatgaaggat
     3301 attagaaaca gttatattga agtatataaa aaagctatag aaaatgaaga gtttgtaaaa
     3361 tcatattttg aatcttatag tttaataagg ggataatgta tggaaaatta ttttgataaa
     3421 caagcaaaag agctttataa taaagcaagt gaaattataa atactcattc tatgctaaag
     3481 gcaaatcgtg ctaatgaaaa atttgatata gatataccac aagattttaa gtatgagttt
     3541 tttagtcttg tagataaagt gaatttaagt cttatggaag aagaagataa tttttatgga
     3601 tattttttgt ttcagatgtc aagagaaata agatttgata taagtagtcc tacaggtgtg
     3661 aattttaagg gagctaaata tgttatatat tttaatccaa taatcttttt aacgcttgac
     3721 ataaagcaaa tggagactac aattaaacat gaaattcatc atatattatc tatgcatttg
     3781 atgagagcaa aggagttaaa aggaaagtat agtacattag caattaatat ggcaatggat
     3841 atagtagtaa ataaatttct aaacaattta ccaccatatg caactacctt agagtgggta
     3901 aatttaaaat attccttaaa acttgaacca tatgaacctt ttgaatatta tgtagaaaag
     3961 attcagactg aattagattt gatggaagtg gatgaagatg gtgaggaaga tgatagtgat
     4021 aaatatggag atatagaaat tgattatagc cccgaaagaa ctcatgatat atgggatgaa
     4081 tccaatgaga tagatgaaaa tacccttaga gaatttactg aaaaatttat taacagttct
     4141 caaaaaggta aagttcctgc ttatttagaa agtatgatat catcacttaa aaatagtaat
     4201 ggtgaattgc catggaattt atatcttaat agaataatgg gaacagttga aaataataaa
     4261 aagaagacta taacaagaag aaatagaaga caaccaaatc gtttagattt gaggggagaa
     4321 cttagaagtc ataaggcaga aatagcagtt gcacttgatg taagtggaag tattagtgat
     4381 gaagagttta aacaatctat taaagaagtt cttagtatag tgaaaaacta taatcatgaa
     4441 attacaatta tagaatgtga taatgaaatt agacgtgtat ataaagtgag aactgaaaaa
     4501 gatataaaag atagaattag gataggtgga ggtactagat tttctccagt ctttgagtat
     4561 gctaataata aaaagattaa tttgttagtc tactttactg atggtaaagg tgaaaataaa
     4621 ctacaagtaa tacctagagg gtataaaatt ttatgggtta tttctggaag aggagataaa
     4681 atttcattaa aagaacctta tggagcagtt aaaaaactta ctaaggttaa aacaaatgat
     4741 gaaatattag aaataaatga tgtaagaaat gatgggtact caatgaatag ccaagcacca
     4801 attttatagg tatttacagg agataagctg tattaattta ttttgatatg cttgttataa
     4861 aaatctaaaa aatacattaa tcaatggtta atgtattttt taattttaat aatataagtt
     4921 ttacttacat ttaagtctta aaatgtgtta aaatataata tatagcagat agttttggca
     4981 aggtggattt agttgtcaaa actatttttt ttattaagga tggtgatgtt tttgtttaag
     5041 attgatgatt atataatgta tggtatgaca ggagtctgca aagtcatgga tataacaaat
     5101 gaacgattta caaatggaat aaaaaaagag tattatgtat tgagtcctat ttattctaat
     5161 aatacagtaa taaaaattcc tgtagataat gaaaaagtac caatgagaaa gttactttct
     5221 aaaattaatg tgctttcatt aataaatgat attcctaata tggatacatc atggatagat
     5281 aatgaaaaat tgagaagtga gcaatttaaa aaaatactca gaggtggtaa atgtgaagag
     5341 ttaataaaat tagtaagaag tatagataat aacagagagt atgtaaaatc tattggtaaa
     5401 aaaacacatc aagcagatga taatataatg aaagaagctg aaaggttatt aaatgaagaa
     5461 tttgcaacta ttttagatat ttctcctaat gaagttagtt catatatttc aagtcatata
     5521 cctcaataaa gtgttttagt atcctttatt taatctattt taagtattat ttatgtatgc
     5581 tggctttggt aaaataaaat atataattaa attatataaa aaattaagaa tgattgtatt
     5641 gaagaattac agaatagtgc ggagagcctg ctcctttagg tgtgagatgg atatcactta
     5701 aagatttaaa ttgttattgt agatgttttt aatataaaac aagattaata agtgtattgt
     5761 aagctttaag tgaaattgat actataatga gttacttaga ttttaaaata tataatatta
     5821 taaaaaatcc ttttaggttt tgaatgaata agatggatac tatgttgaat agaatccatc
     5881 ttatttaagt tttatttatg ttgtttattt gtaagcatta tttttgtaca tgaaaacaca
     5941 gtgtaaaaat atgcttagaa ttatgaagtt ttattaaaaa catattcgac aaaataagac
     6001 taaaaaatat tcttaaactt ataacta
//
LOCUS       DAEMHW010000205         6025 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEMHW010000205 DAEMHW010000000
VERSION     DAEMHW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.205"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(184..2778)
                     /gene="clpB"
                     /locus_tag="KPK67_003261"
     CDS             complement(184..2778)
                     /gene="clpB"
                     /locus_tag="KPK67_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBG2460185.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGKGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(2815..3411)
                     /locus_tag="KPK67_003262"
     CDS             complement(2815..3411)
                     /locus_tag="KPK67_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460186.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(3428..3772)
                     /locus_tag="KPK67_003263"
     CDS             complement(3428..3772)
                     /locus_tag="KPK67_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBG2460187.1"
                     /translation="MNKSKYMNVCFKNYIKVVEGMYTKRDLIPLKLINSMDKNNKIRK
                     TLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYMINPHIAYKGSRADKESLLAE
                     YNQIKRPNKVSK"
     gene            4199..4573
                     /locus_tag="KPK67_003264"
     CDS             4199..4573
                     /locus_tag="KPK67_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2460188.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            4570..5433
                     /locus_tag="KPK67_003265"
     CDS             4570..5433
                     /locus_tag="KPK67_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460189.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLKVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            5433..>6025
                     /locus_tag="KPK67_003266"
     CDS             5433..>6025
                     /locus_tag="KPK67_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG2460190.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNINNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFV"
BASE COUNT         1986 a          922 c          692 g         2425 t
ORIGIN      
        1 tattcaaatt atgattatat tataagaaat ttactaaaat acttcctttg agttaaattt
       61 aatttggaca tattgactta aaaatgtttt attaatttga taaaatattt cttttcctat
      121 agtttataaa taaaaatcag ctgagcaaaa tatttagtat attatgctca actgattctt
      181 ttattattta actaaaactt caatattttc atcttttcca tctataacta tagtacatcc
      241 attatacaca tctccagcaa taagtttctt tgcaattata gtttctaatg tatttcctat
      301 atatctctta agaggtcttg ctccatatac agggtcatat ccttctctca ccataacatc
      361 ttttgctgaa tttgtaactt caatagtaat atctttttct tttaatctat tttttaaatc
      421 tttcataaat atatctataa ttttcttgat attttcttgg tctaaaggct taaacattat
      481 aatatcatct actctgttta aaaactcagg tttaaatcta tgcttcattt cattcattac
      541 taaattgtta gtagtctctg ttatatttcc tcctgcttct aatagataat tacttcctat
      601 attagaagtc attattataa gagtattttt aaagtctact gtcttacctt tattatcagt
      661 aagtctacca tcatctaata tctgtaagaa catgttaaat acatcctcat gtgctttttc
      721 tatctcatca aatagtatta cagaataagg agcacgtcta actgcttctg taagttgacc
      781 accctcttca tatcccacgt accctggagg aggtccaact agtcttgata cagcatgttt
      841 ttccatgtat tcagacatat ctattcttat tatattatct tcactatcga ataaatttct
      901 agcaagagtt tttgcaagtt cagttttacc aacacctgta gggcctaaga aaataaatga
      961 accaattggt tttctttcat cttttaatcc tgctcttgca cgtattactg catttgaaac
     1021 tgctgtaact gcttcatctt gtccaataac tcttttatga agttcatctt ctagtttaag
     1081 taatttttct ctttctcctt caactagttt agttactggt atacctgtcc actttgatac
     1141 aatgctagat atttcttctt cagtaacttc ttcttttaaa agagaatttt cagaaccgtc
     1201 attcatattt tcttcgtact gctttatttg ttcttctaac ttaggtattt caccatattt
     1261 aacctctgct gctttgttga aatcatattc tctttcatat ttttcagctt tacctttagc
     1321 ttcgtctaat tgagccttta agtttttaat atctaatatt tgactctttt ctttttcata
     1381 ttttgctgtc atttcatcat ttttagattt taactctgct atttcttttt gaatatcatc
     1441 caatcttgat ttacttttat catcattttc tttaagtaat gcttctcttt cagtttctaa
     1501 tgtaaataac tttcttctaa caacatctaa ttctgttggc aatgaatcta tttcactacg
     1561 aatcatagct ccagcttcat caattaaatc aattgcttta tctggtaaaa atctatcttg
     1621 tatatatcta tgagaaagtt ttgctgctgc tacaatagca ttatcatgta ttcttactcc
     1681 atgatgtatc tcaaatcttt cttttaaacc tctaagtata gatattgtat cttcaacagt
     1741 aggttcttct gcaattacag gctgaaaacg tctttcaaga gccttatctt tttctatata
     1801 ctttctatat tcatcaaaag tagttgcacc aatacagttc aattcacctc ttgcaagcat
     1861 aggtttaata agattacctg catccattga accttctgtt ttaccagctc caacaatagt
     1921 atgaatctca tctataaaca gtataatttt tccatcagaa ctttgaactt cttttaaaac
     1981 tgcctttaat ctttcttcaa attcacctct atattttgca ccagcaatta aagctcccat
     2041 atcaagtgag aatacaatct tatcttttaa accttctggg acatctcctc ttactattct
     2101 ctctgctaac ccttctacaa ttgcagtttt acctacacct ggctcaccaa tcaatacagg
     2161 attatttttt gttcttcttg ataaaattct tataatcctt cttatttctt catctctccc
     2221 aattacaggg tctaatttat ttttcttggc taaatctact aaatttgttc catatctagc
     2281 aagtgcatca tatgtgcctt ctgggtcttg tgtttctact ctttgactac ctctcacttg
     2341 tgataaaata tttaaaaagt cattcttatt tatattatat tgttttaata ttttaccaac
     2401 agctgatttt gattctgttt ccatcattgc aagcataaca tgctctacac ttatatatga
     2461 atctttaaat tctttagaaa tactctctgc ctttataagc acctcattta tttttctagt
     2521 agctgatact ccttgtgagt cagcaccttt tccatatact tttggaagct tatcaatttc
     2581 aaagtttaca gtatttctca aagaatcaat tgatatattc attttttcga ttatatttgg
     2641 tatcaatcca tcctcttgat taataagtgc tgagaaaaga tgaattacat ctacttgttg
     2701 gttatgattt tttactgctt cgttataagc ctcatttaaa cttttttgaa ctcttaaagt
     2761 cattttttct acgtccatat aaatccctcc attttaatat tatatccaaa taaattaaat
     2821 tctctcattt ataattattg attttctaag ctctaaaaga ctatcaagta aattctcgtt
     2881 tttttgtata tcgaaactca atgttaaatt aaatgttcca tctgaattaa aaaaatcgaa
     2941 tacttctttt gattttgcta ttaaatacaa gtcttcatct ataagtcttt tttcacctat
     3001 taataaatac tgacatttat ctataaaact ctttttaaaa tcaatagtgt atagattttc
     3061 taaaatatta tattgataaa tagcaatatt attaaacact tttaatatgt ttgaatttac
     3121 aaaattgtaa atttgattat tgttatacag tgatatttgt tcgtatatat attccctaaa
     3181 taaaggatga atgttttgct taagttttaa agtaacttca gctggtattt caaaataaag
     3241 cttccagtac ttagagttat ttttttctaa aataaaagct actttgttca aactggtaga
     3301 actaatctcc tttagtgtac aattaaaatt atagtgtctc aaactagttt tttcattctc
     3361 aaatacatag tcaaaccact gtagaacatt tctatcaagt tttaaactca tataaaaacc
     3421 ttcttcctta tttactaacc ttattaggtc tttttatctg attatattct gctaataaac
     3481 tttctttatc agctctacta cctttatatg ctatatgtgg atttatcata tactcaccat
     3541 ttttcactct ttgtaaaaaa tccatttttt tcagctccgt aaacaatgaa gataaagaag
     3601 tttttggata ttcaaattca caagataatt catataatgt ttttctaatc ttattatttt
     3661 tatccataga gtttattaat tttaatggaa ttaaatccct cttagtatac attccttcta
     3721 ctactttaat ataattctta aaacaaacat tcatatactt acttttattc ataactaaac
     3781 ttttgctctc tgtatattcc tgatttaaat ccttttttaa tgtcttatct aaattaaata
     3841 tctcttctca ctctccttat ctctaattga taatataaat atattacaaa aaaatatgta
     3901 tgtcaaaact gaaaatgcaa aaagttcacc ttataagtaa acttttattt agctataagc
     3961 taaactttat tattttttta atttagtatt ttcaatttat tggcaggatt tttgaacaat
     4021 atttataaat tttttaaaaa aatttatatc tatttaatat ggcagccttt cataaaatac
     4081 attacattta ttaaatgtat aatattggca attagttttt tctaaaatca gttgacatat
     4141 tatatatata tttatataat gtgtatatac tatatataca atttaaggag gtgtcttatt
     4201 gaatatagtt attagtaatt cgagtgggaa gccaatatat gaacagatta ctacacaaat
     4261 taaaagtatg attataagtg gagagttacc agaaggctca gctcttccaa gtatgagact
     4321 attggcaaag gaacttagaa taagtgtaat tactacaaaa cgagcatata gcgatttaga
     4381 acgagacgga tttattgaga cagttactgg taaaggcagt tttgtatcta gtaaaaacat
     4441 ggattttgtc aaagaagaac aatttcgttt aattgaagaa tacctacaaa aagcagttga
     4501 tacagcaaaa agtagtgata ttacatatga cgaattaaaa gatattttat ttttattata
     4561 taaaggagaa tgataagtat ggatgcaata actataaaaa atttaaataa aacttataaa
     4621 gattttagcc tacaagatat atctttttca gtaccaaaag gaagtgtaat gggctttgta
     4681 ggtgaaaatg gtgcaggaaa aacaactaca ttaaaagcaa ttttaaattt aatatcttat
     4741 gatagtggaa atatagaaat ctttggtctt gatagtaaaa aaaatgaaaa agaaattaaa
     4801 gagcaaattg gggttgtatt tgagggaagc aactttcatg aaaatttaaa cactgaccat
     4861 gtttcaaaaa tcatgagtaa aatctataaa aattggaatg acacattatt taaagactac
     4921 ttaaaaaaat tgaaagtacc agacaataaa ttaattaagg agttttcaaa ggggaataag
     4981 atgaaactta gtattgctgt agccttatct cataaaccaa aattattaat actagatgag
     5041 gcaactagtt cacttgaccc tatcgttcgt gaagaaatac tagatatatt tttagatttt
     5101 atacaagatg aagaacactc tattatctta tcttcacata ttacaagtga tttagataag
     5161 atagcagact atattacatt tatacataaa ggaaaaattg tatttagtga aaataaggat
     5221 gaattaattg atactatggg agttttaaaa tgtaaaccta gtgattttga taacctttca
     5281 agagaggatt attcatatta tcgtaaaagc caatttggat atgaagtact tcttaaagat
     5341 aagcatagat ttatctcaag acatccaaac tgtattgttg ataatacgtc tatagaagaa
     5401 attatgttat tttatgtgag aggtgataaa taatgaaagg tctaatttta aaagatttac
     5461 ttaatttgaa aggaaatata aagtttatac ttttattcat cattatgttt ggctttatgt
     5521 cttctttagg agatggaaat attaataact ttataggtgt tattatagtt ttatgtacta
     5581 caatgattgt ttcaacattt tcatatgatg atttgaataa atgggatagt tatgtactta
     5641 caatgcctat taatcgcaat gatattgtat taagtaaata tttaactatg ttaatattta
     5701 gctttatagg tgttcttgtt tcacttattg ttagtgtaac tattggttat tttaaaaata
     5761 cacttatatt aaatgaaaca ctacttatta atgcactaat cttatcaatt tcagtatgtt
     5821 ttggtagcct tattctacca cttatttata aatttggtac agaaagagct cgtttattaa
     5881 tgattttatg ctttttagtt ccaacactag ctttattagt ttttaaaagt attttagaaa
     5941 atattagttc acctatttct atagaaatta tacttaatac actagtatat tctcttccat
     6001 ttgttgctat tttactattt gtgat
//
LOCUS       DAEMHW010000206         5963 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEMHW010000206 DAEMHW010000000
VERSION     DAEMHW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.206"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(34..891)
                     /locus_tag="KPK67_003267"
     CDS             complement(34..891)
                     /locus_tag="KPK67_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBG2460191.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCGGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEKYRRIFK"
     gene            complement(1208..2536)
                     /locus_tag="KPK67_003268"
     CDS             complement(1208..2536)
                     /locus_tag="KPK67_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG2460192.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2684..3013)
                     /locus_tag="KPK67_003269"
     CDS             complement(2684..3013)
                     /locus_tag="KPK67_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2460193.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3037..4316)
                     /locus_tag="KPK67_003270"
                     /pseudo
     CDS             complement(3037..4316)
                     /locus_tag="KPK67_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(4332..4658)
                     /locus_tag="KPK67_003271"
     CDS             complement(4332..4658)
                     /locus_tag="KPK67_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2460194.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4793..5422)
                     /locus_tag="KPK67_003272"
     CDS             complement(4793..5422)
                     /locus_tag="KPK67_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460195.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5637..>5963)
                     /locus_tag="KPK67_003273"
     CDS             complement(5637..>5963)
                     /locus_tag="KPK67_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2460196.1"
                     /translation="SSNYNRIFKDIKYVSDELFLVFNIEKKYDKVIGLMMHLAFMVDG
                     LIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDKKYNIEINEKECYQILLILEYAEI
                     IEKDYQ"
BASE COUNT         1847 a         1091 c          633 g         2392 t
ORIGIN      
        1 taataagtat ttttaggatt ctgttacata atattattta aatattcttc tatatttctc
       61 ttcttcttca gtaaacacat taaatacaac ttttaaattt ttttcaggat tttcttttag
      121 ccatagtcta actctactta cagcaagatt agctgcctct ttttttggat aaccaaatac
      181 acctgtagaa attccacaaa aaacaatatt ttttatttca tcaatttctt ttattgtatt
      241 taaacatgat ttataacaat gaagcagttg cttttcttgc tctttacttg gctgaccacc
      301 agatacaata ggtccaacag tatgtactac aaattttgca ggtaaacaat aacctctagt
      361 tatcttagca tctccagtat attctaaatg cccttgtttt tttattattt tgtcacaatc
      421 ttctctaagt ctaggacctc cgcatgaatg tatctcatta tccacgcata gatgtaatgg
      481 ttgtaaacac cctaataact tgttatttgc agcatttact attgcatctg ctcttaaatt
      541 cgttatatta cctctccata tggcaatccc ttcctctact tcttctatat catttacatc
      601 tactaactct ctttcttcac attcaatcaa caataaatta tgtaacatat taagtatctc
      661 tggagcaatt tcttttggtg gatatacatt caaaagtcct ctaagtatat ctctaggctt
      721 aatttcatca tttgataaat tgtatatttt agaaggaact tcttttgaaa aatatgcaat
      781 taaagtgttt atattttttc ttctttgttc gtcagttaga ggcttgatag ttttatcaaa
      841 atcctcaaat aaatttacat catcagcata atctctccac ttcatagcca tctctccatt
      901 cttgtatttt agacttaaaa ataattatgt atttatgtac atatttattt atttctataa
      961 acacctacat aaatcctgtt aaaagtgtaa attttttatt atgcaacttc tctggttatt
     1021 tctattgatt gatagtaaat tattgtacct aaatttaatc caactaacaa gtaactaaac
     1081 ctactttcct aactgcaaaa tacactatgt ttgccacttg tatctcattt gtaaataaat
     1141 aaagtcccta caaatacatt aactaggcta tgtactaaac tgtaggaact ttaaaaatat
     1201 attattctta attaagtttt ggccaataat ttttatttgc ttctattaaa tcatctagta
     1261 attcttttgc aactgttgca ctaggaactg tcttagatag cataagtgct tgccacaatt
     1321 tattataaga cttctcaatc caagcatcaa ctactaattt ttcaactgct aattgttgtt
     1381 ctattaaacc tttttggaat gttggtattt tccctattga taatggttca tacccgtctt
     1441 ttccaactat acatggaatt tcaaccatag catcatcttg tatattttct atagctccat
     1501 tattttgaac tatcaataac atacgctcct gtgtattaaa agccagagct gtagctaaat
     1561 ccactataaa ctctgcatgt attcctatct taagcccact gcccttagaa tgtccttgct
     1621 tttctatcaa tgcacatgaa ccaaaaactt ctttttctct tccatcaata acttcattgg
     1681 cacgagtata atttacatca gaatgattta caacataatc tggaaataga tagtacttta
     1741 agtaactact tggtattgta gttaaatctg tttttactat ttctttagta aataaattag
     1801 tttcaaacca actatctttc ttgtcatttt tcattccttc ttcaccattt ataaaaccat
     1861 attcttttat atgttctact aattttggta aaagttcatt tcctttttta tctttaattg
     1921 aagtatacca accaaagtga tttaatccaa agtatcttat atccatatgt ttacgagatt
     1981 tgagtcctgc aatccttgct atattatgtt ccatacctat tggcatatca caaatattta
     2041 ttaccctaga attaggtctc aaccttctaa cagcttccgc tattattgat gctggattag
     2101 aataatttaa catccaacaa tttggagaat acttttccat ataatctatg ttttcaagaa
     2161 caccttctat agacctcatt ccataagcta ttccacctgg tccacaagtt tcttgaccaa
     2221 ctaccccatg ttttagaggt atcttttcat ctagttctct catttcaagt tttccaactc
     2281 ttatatgagc taaacaaaaa tctacatctg tataagcctc tttagggcat gttgttgcta
     2341 aatactctat attagggtcc ttttccttta ttagtatttc acaagcttta gctaccttat
     2401 tttgtctttc ttcatcatta tcatataatt tgattgctct taatggtaac ctatctaagt
     2461 tatctagcaa cattaatatg atttctgggg tatatgtact tcctcctcca gctattacta
     2521 ctgaaaattt ttccataata atatctcctt ttctatttaa aaatttactg tatttataaa
     2581 taaatttata aatactagta ctttaaagta aaagggagtt catttattaa atctattgat
     2641 ttaataaact atagccaaac ttcaagcact atttacaaga aatttaattc ttattaattc
     2701 ttgggtccat catctcaaat atatctacaa gttcagatac taaagaatga gcaaataatg
     2761 catttgaata atggtcttca gagtgcacaa ctaaaaaatc agtcccacta tcttcactat
     2821 tcatagcatt tgatagtaat tctaaatgat gctttgatgc tgtttgtatt ttctctgagg
     2881 ctgaatttaa aaggtgtttt gctttattgt agtcaccttt ttttatttcc ttaagagaca
     2941 tcattacatc acttttagca tctcctgctt ctgatattat actaaatact ttttcttcca
     3001 tttcaggtac catatccttc tccttctaat gacttgttaa aaatcaaagc tatcatcatc
     3061 taatgttgta gctaattctg catttttatt taattcttcc ttctccatta tcttaaagaa
     3121 tggataatat atcaaggtca taagtattat acttgcaaat actaatatga ttgccttcca
     3181 atctaatgtc atcaaaaatg ctgcaacccc tggaggtaaa aagcctggtg gttctgcaat
     3241 aggtaatcct actaatccta cttttaatgc cataaaatta agtgctccaa ccaatacagg
     3301 tccaaataca aatggtacaa acatatatgg gttcataact attggtgttc caaatattac
     3361 tggctcattt attccaaata gtgatggtaa aatcacaact tttcccatac tattaaaact
     3421 cttacactta cttcttgaca taaggtacac taatcccaaa gttattcctg aaccagttac
     3481 ttgcaagaaa ccatagtaaa atcctattgt aaacacatat tttgggtctc ctcctgctgc
     3541 tatcagtgtt gcattttctg ccatatatgt agctgccata tacttgaagt tattgctgct
     3601 ccatgaagcc caaagaacca taataattga gttataaata gtattatagt tactgcaaaa
     3661 acattatcta cactgcttat agctggtgct aagaaattca ttataatagc tggtagtggt
     3721 tgacctgaga catttttagt tattgctgct attgctgtaa ataacattat acatactatc
     3781 attggagtta agtttctaaa acttttattt actacactag gtacttcttt tggaaatcta
     3841 attacgagat ttttatcatt cataaactta gtaatatcaa ctactaaaag tgctactaat
     3901 atacttacaa ataaaccttg tgcatctaaa taacttgtat ctaatacacc atctactact
     3961 tgtccacata caacaagttg tgcaacaact gctgtcatac ctatttctac atgattaaga
     4021 ttatattttt tagctagtgc ctgagctata aaaaatgctg aatatataga taataaactc
     4081 atagtaaatt tattagggat aagtaatgta tccatatttt cagataataa tgtttttgca
     4141 cttccttcag gtaacatatt gtttaatgcc ataaataaca tacttaaaga accaacaatt
     4201 attattgggg taatgcttat cataccttct tttatagctt gaagatgcct ttgtttttca
     4261 atcttttgtg ctattggaag aagaaatttt cttaaattat tttgaagtgc ttccattttt
     4321 attcccccca attatctatt tttatataat tcgattgccg acttcataac agaaggagca
     4381 tccatacgac cataatgctt agtatctatt aaaccaactg ctattttgta attcttacaa
     4441 gcttcaacta catcatcttc cttaaaccta atttgtggtg ctacaagtac tacatctgta
     4501 tcattagctt caatgtatga ttttaaattt tcaaatgatg ttgcctctat atttatagtt
     4561 ttagttttta acttttcatg ctcactacaa aaacttttca tcttttccac aagtacacct
     4621 gttgaagctc catttgcaca tactaataaa atattcataa ttaccctcct acaatatcta
     4681 acctcttttg ttttctaatt ttttatcagc caattcttac tctaattcct atcagttttt
     4741 actttaattc ttattatttt attttaattc ttattaatat ttactctaat tcttagtact
     4801 gccaatgatt aaacttgaaa gccgtatcta taacttcttc ttgcttatat ttttctaata
     4861 tttttaagta tctttcttta taatcattat ctacttcttt ttctggtatt actatactag
     4921 cttcatttaa aaagtggaat gaatcccttc ccatttctaa tcctctatat gtatgcttgt
     4981 caagtgccac gtccataact tctggaactt tacccattgc aaatcctttt ataattatat
     5041 tttttaataa atcacttgat ctatctttct cacatgcaca aagatatctt attgcatgta
     5101 tgaaaaataa aggtctgtct ccatctgcat atgaatattc ctttcttact tgatttaaat
     5161 tatttatgat tattacagct gatgggtccc ccattcctat atcttcaact gatattgtaa
     5221 gtatccttct ccatagctta tcttctaatt ctggtgatga tatatatagt tcataagcaa
     5281 attcacaagc ttcttccacc atatttcttc ttatagcttt ttgaaatgac gatattattt
     5341 cgtctgcact aaatccgttt cttgtagtca ttcttgccca tgggtcattt gcctttttat
     5401 ctcttaatat tttatcttgc atattattta ctccttttta tttaaatttt aattatttat
     5461 attactcatt taataatata agcaagtatt atgccagttt ttttagacac tttaaaacac
     5521 tgttatttca atcattttta tcatcaaaac ttagatactt tgtctaagta tatttatatg
     5581 tgtctaatat taatattatt ttagactaaa aaaaattacc aaataagttt ttatactcat
     5641 tggtaatctt tttcgattat ttctgcatat tctaatatta ataatatttg atagcactct
     5701 ttttcattaa tttctatatt atatttttta tctaattgag atactatatc ttttattttg
     5761 cttaggctct tgtgataatc aagtgttttt tctttatcaa gcttttcaat ttctctagta
     5821 tttccaatca acccatctac catgaaagct aaatgcatca taagtccaat tactttatcg
     5881 tatttctttt ctatattaaa tactaaaaac aattcatcag acacatattt tatatcttta
     5941 aatatcctat tataattaga gct
//
LOCUS       DAEMHW010000207         5951 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEMHW010000207 DAEMHW010000000
VERSION     DAEMHW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.207"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            88..175
                     /locus_tag="KPK67_003274"
     tRNA            88..175
                     /locus_tag="KPK67_003274"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:122..124,aa:Leu,seq:caa)
     gene            complement(362..805)
                     /locus_tag="KPK67_003275"
     CDS             complement(362..805)
                     /locus_tag="KPK67_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460197.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICVVVVYNKLDKKL"
     gene            complement(808..1005)
                     /locus_tag="KPK67_003276"
     CDS             complement(808..1005)
                     /locus_tag="KPK67_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2460198.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(1197..2063)
                     /locus_tag="KPK67_003277"
     CDS             complement(1197..2063)
                     /locus_tag="KPK67_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2460199.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(2289..3158)
                     /locus_tag="KPK67_003278"
     CDS             complement(2289..3158)
                     /locus_tag="KPK67_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG2460200.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(3664..3885)
                     /locus_tag="KPK67_003279"
     CDS             complement(3664..3885)
                     /locus_tag="KPK67_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460201.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(4375..5355)
                     /locus_tag="KPK67_003280"
     CDS             complement(4375..5355)
                     /locus_tag="KPK67_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460202.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(5519..>5951)
                     /locus_tag="KPK67_003281"
     CDS             complement(5519..>5951)
                     /locus_tag="KPK67_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2460203.1"
                     /translation="RIYRGNSHLNLYGLIIPLECYPYLFANNKKLELSDNFVEDNLGK
                     VNENEYNILLAHTPFFFEKYAKWGADLVLAGHVHGGIIRIPFVGGVLSPNREFFPKYD
                     WGKYEKDNSTMILNKGLGGSKVLIRLNCKPEIVKITLKYEK"
BASE COUNT         2030 a         1014 c          564 g         2343 t
ORIGIN      
        1 tttaatattc ccataataat aatagtatat taaaattaaa tatgttgcaa acaatatatt
       61 tttttgctat aataatatac atgacatgcc cgagtggtgg aattggtaga cgcagtggac
      121 tcaaaatcca ccggactaat actccgtgcc ggttcgagtc cggcctcggg caccattaaa
      181 aactcctaaa gctatttatt caatagatta ggagtttttt tatttttgat ttaaatttta
      241 agttattttt tgaaattact gtgtcttttt aaaatttata taattatata aatttatctt
      301 ctagcattaa tttaaaaact aatttatttt tatataattt tctattttat atcataactt
      361 attacaactt tttatctaat ttattgtata caacaacaac acaaataaat tgaaataaaa
      421 taagcaatcc aagaacaact aaaacccact tgcttattac cattacagaa aatacactca
      481 atatggaaac aataaatata agaattacaa gaacatagga catgatttga ttagtgatac
      541 gtccagcttt atcacgtaac ataattcttc tttcatcatt catctctatc ctttgatttt
      601 ttatcttttc ttcataatct actgctcttt caggtttact ccaatacata tattgaagaa
      661 tcatacatac acctggacct aaagcagctc cagataatcc ccaaataaaa gcctcaaatg
      721 aaaactcagt aaatgtagcc aataaaacaa gtaaaattcc acaaataaaa tatataattc
      781 caacatataa catattcttt ttcattttta ctcctcctca taaataaata tttcttcaat
      841 actcatatca aagtatctag ctatcttaaa ggctaagatg atagaaggat tatacctacc
      901 attttctaat gaacctatag tttgtcttga tacttgcaaa atagaagcaa gttcttcttg
      961 ttttatgcct ctttcttttc ttatttcttc taatctattt ttcacatcta tcccccttta
     1021 atatctcaac tacggaaagt ctactttcca taattataca actatattcc atttatgtaa
     1081 agcttgcttt ccatattcac aaaaaaataa cagactattt tcagcctgta attttagaat
     1141 ttcatgttgt ttattaattt taatgttatt tattaattcc atattattta ttaattttaa
     1201 cttggtttct gaattggaat ccaaacttct gtagtataat caggagcatt gatatcgcca
     1261 ttgggataaa gttcaatatc tggtaaatta gcatacacat acccagaact tggtaaccac
     1321 tcagtaataa ttcttttttg caattcctgt attgccaaag catttggcat tggagaaaca
     1381 cattcaaata ctgcccatgt acctgatggt attaagtatt catgagtatc ttctaataca
     1441 ggcttatctg ttggtgcagc aatataataa tctaaaccct tactatttgt acacacacta
     1501 actccaagca atccacaggg ttcattattt attaactctg aaagtttatc aattgttcca
     1561 ttttttatgc atttagccca taacttagga actttctcaa aacactcttc tacaatcatg
     1621 ttcatacttt cttttatacc aacaatacga aaccctttct tttctacaat acgatattgc
     1681 atttctatat ttccttttac ataaatgctt aatgtcatct taggatatgc ttttaaaaca
     1741 acaccctttg ctctagctgc agatggagaa atgttatgta tattttgaaa agctctatta
     1801 aaagatgttg gtgaatcgta accatacttc tcagatagtt ctaaaatttt gatgttactg
     1861 ttctgaagtt caaatgcagc cttagtcata cgtcttcgtc taatatattc agataatggt
     1921 acttctgcaa tatatgaaaa cattctttga aaatgaaagg ttgaacaaca tgcaattttt
     1981 gctgcttctg cataatccac tccactttcg agattttctt caatataagt aatagcttga
     2041 tttaatttat ttatccattc catttgatat tacctccaag caaattatac agtttaataa
     2101 aataatatac ctctctattc atgcacaaaa ttgtaagcat ttaaattcat aagtattttt
     2161 caatcaatac aataaatata aaaattgtat tatacaataa aaaagttcaa taacattata
     2221 ttataaaatg ttattgaact ttttataaaa attaatttaa gtgtagttaa attaagtatt
     2281 acactatctt atatattaca atatcctact atcttatagt cttctggcac tttataatct
     2341 ttgtctggag tacctaaatt ccattttaaa tcaaacatag ttgcacttat tattgttgca
     2401 aaataaagca taactatacc aatgtctatt ttttcttcat ataaattagt atgaccatct
     2461 tttctaacag ctaaaactac agtgcctcca tcaactataa atctccatgg ttgtctattt
     2521 aatgtagatg gagctaatct tgcatagtga aatgcatcaa gaagtgctct ttcttctaag
     2581 atttctatac ttgcaacatt tccccattcg ttcatataaa caacatcttc tacacctagt
     2641 ctagtagacc ccttatctgt tacaacttgc atatcagatt ttgaataatt ttgcccagtt
     2701 tttgtagcat ttaatacttt agcattactt acattttctc catatccaat agctataatt
     2761 cctgttacct ctttatctga attaattcca agtttttctt ttaccttttt tccatcttca
     2821 aaagtaaccc aacaagaacc aattccaagt tctgttactt taaagattat actttctcct
     2881 atgtaccctg aattttcaat gtaattatct tttatgtcag ataatattat aatataactt
     2941 ggagaatcta tcatatttcc tttgtaccct gctactttat ctaaattatt aaaaacttca
     3001 cttctattca taattcttac atcaacatct atttctggaa ctagtttctt acaagattct
     3061 gagtatttct taatttcact aagatattct tctttaatat ctgtgttctt atattctcta
     3121 acagactttc tatttaatat gattgtttta taatccataa ttttatcctc cccaaactag
     3181 tattgtctta attatatacc cattaatata ttattttact catttattta ataaataaag
     3241 attacaattt tgtaattatt atttattaat aatattccac ttttacatta ataatgacca
     3301 gttgtgcaaa atccattgcc aaccaccaaa taaaaacata ttataaaggt aatataaaat
     3361 aacaaacaaa tcttaacaaa aaattaaact ttttttacac gaaagaaggc ttcccgttgc
     3421 cttctttatt tttataaaaa aataagctgt ttctaaaatg atttcacatt tttgaaacag
     3481 ccaagttatt ctataatgac aactataatt tgaggatgtg agggagagtt gtcattataa
     3541 atttagggta attatattat gttttaagct agttctttct atatttatat aatacatttt
     3601 atatatgaat tttatatgag attggtataa atgatatatg aaattacata tgtttatatt
     3661 atattaaatt ttattattac attttccaca acaatactca tatgtctcca cattatttgg
     3721 aatatttaca aatacaaatt ctttacagtt ctcacaatac tttaatatag atatagttcc
     3781 acaagtgtaa cattcaaata ttacacaatc ctcattttcc tctactattt caaacttttt
     3841 actatgttca ccacactctg ggcacttgtg attatttaca ttcataaata ctcctcctta
     3901 ttagtatagt tacatgtcaa aaacttaatt aatgtattct atattactta ttatatattc
     3961 tataataaat catattctaa tataataaaa caaattaaat aaaaaaaaac cataaatttt
     4021 aaatttatgg attaattctt gtaatgataa ctctattcga ggatgtgagg gagggttatc
     4081 attacaatag ggtattatat tatggttttt aagctagtct ttctacacct catatgatac
     4141 atatgctata tgaattttat atgattttta cataaatatt ttatgaaatt taaatttttt
     4201 agtcattttg aaatttgtaa aaagttctct aaaaatccac agcatcataa acagccaaaa
     4261 tttaagtaaa acatcataaa aaaatttgtc caataatttt aacaagtaat ctcacctcat
     4321 tataattatt gacacaaact taaataaaca atctataaat cttaaacttt ccccctattg
     4381 atacacaatt tctagtttgt caattttatt ttgcatttct aagaaaattt cttcaacaga
     4441 attgttaaat atattgccta aatagattct tttatctttt tgaaatgaat aagattttac
     4501 actaccatca gaagaaatat ataaatgact ccactctgct ccatattttg aaaacctctc
     4561 ctttgtatct ttaaacctca tatcttctat ttttattgct ttttctctgt ctcctcttac
     4621 tacagtgtga ccccttttta ttaaactggt tatttttata tcttctttta atttaatacg
     4681 tgtatcatca ttttttgaat atactttcca tatcacttta tgacgataat tatctaattc
     4741 ctttattaag cttctatcaa tagtaaaact atctttagtt accacaatta aatcaaaatg
     4801 gtctaacata tattgaaata tttctctcat acttttattt tttaaaatat cctcaccaat
     4861 aacctctata aatctacagc ctttctcata catgcagttc aataaacttg tgatttcaga
     4921 aaaataatca tttgtattat tatctccatc tatttctata gtaaagtaca aaggagtaat
     4981 gtatgattta tttcctgtta tctcaaatac acttgatagt gtctgtatat catcttcaaa
     5041 cgttaagtat acattctcat aaaacataaa aaattccata actttttctt tcatattatg
     5101 gatatttcta tatctattgg ataatatatt tattatatcg tcagtagtat ttttcccatc
     5161 acattcagcc agtataacaa tttgcttcca acttaagcta tccacatttc cgatactttt
     5221 tttatatcca tcttcttcat cataaatagg accaattata atgttttctt ttggccacag
     5281 atgaacccta gttccatctt ttaatttcgg atatttattt tttacagact cataaaatct
     5341 acttgtatca cccatgattc tttactaccc cccatagttt aaatattcta tgctaaaaat
     5401 agcactttat ttttattaat tcaacaatta ttcaaaattg gtcattatat atgtttaaat
     5461 ggatttaaca tcaaccttat ttatatgtta ttgatgttta tatatgttat ataaaatctt
     5521 atttctcata cttcaatgta atcttaacta tctctggttt acaattaagc ctaattaaaa
     5581 cttttgagcc tcctaagcct ttatttaaaa tcatagtgct attatctttt tcatattttc
     5641 cccagtcata cttaggaaag aactctctat ttggagataa tacacctcca acaaaaggta
     5701 ttcttattat acctccatga acatgtcctg ctaaaaccaa atctgctccc cattttgcat
     5761 atttttcaaa gaaaaatggt gtatgtgcta aaagaatgtt atattcattt tcattaactt
     5821 tacctagatt gtcttctaca aaattgtcac ttagttctaa ttttttgtta ttagcaaata
     5881 aatatggata gcattccaat ggtataatta aaccatataa attaagatgg ctattacctc
     5941 tatatattct t
//
LOCUS       DAEMHW010000208         5917 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEMHW010000208 DAEMHW010000000
VERSION     DAEMHW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.208"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            104..916
                     /gene="modA"
                     /locus_tag="KPK67_003282"
     CDS             104..916
                     /gene="modA"
                     /locus_tag="KPK67_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2460204.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            926..1717
                     /locus_tag="KPK67_003283"
     CDS             926..1717
                     /locus_tag="KPK67_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460205.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFLSLTCLMLFEIFFNREVDKN"
     gene            1719..2414
                     /locus_tag="KPK67_003284"
     CDS             1719..2414
                     /locus_tag="KPK67_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2460206.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMSVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALISEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2401..3132
                     /locus_tag="KPK67_003285"
     CDS             2401..3132
                     /locus_tag="KPK67_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460207.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGNTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            3446..4246
                     /locus_tag="KPK67_003286"
     CDS             3446..4246
                     /locus_tag="KPK67_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG2460208.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            4786..5502
                     /locus_tag="KPK67_003287"
     CDS             4786..5502
                     /locus_tag="KPK67_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2460209.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT         2293 a          722 c          843 g         2059 t
ORIGIN      
        1 acataacaaa acattataaa acataacaaa aacttagaca tcgttttctt agtatgatat
       61 actaaaaaag aatttacact aatttactaa gggggaatat cttatgttta aaaaactttc
      121 agtactagct ttagctatga ttatttcatt aagtattact gcatgtaatt ctaaagaaaa
      181 caaagctaat gatgaatcta atactaaagt gacaacagct gatgattcaa aagaaaaaga
      241 gactcttttt gtctattcag gggcaggttt aaagaaacca atggatgaaa tagcaaaaaa
      301 atttgagaaa gaaaataatg tcaaagttga atatagctat gcaggttctg cacagctaat
      361 agctcaaatt gagacaagtc acaaaggaga cgtatttatt gttggttctg aaccaattta
      421 ttcaaaggcc tttgaaaaag aacttgtagg tgaacataaa acggttgcac atcatacacc
      481 tgcaatagtt gtacctaaag gaaatccagc taatattcaa aaattggaag acttaaataa
      541 agatggagtt aaacttattt tgggagacaa agagtctaat gctataggta agactacaca
      601 aaaaatactt gaaaaaaata atttaaaatc tataaatgat aatgttgtct ctacagctgc
      661 aactgtaaat gaaatgattg tacagttaac atcaagtaaa gctgatgcta caatagccac
      721 aaaagattcg gtctttggca atgaagatgt agaagttata gaaattgccc aagataaaaa
      781 tatagaccaa ctcatatcag caggaatcgt aaactatagt gataaaaaag atttagctaa
      841 taaatttata aattatatag agtcagacga agctaaacag atatttaaaa aatatggttt
      901 tgaacctgtt aagtagtgat taaacttgaa aaataaattt tttactacac tacttactgg
      961 attttttgct atagtctttt tgtttattgt atcaccactt cttatgttaa tcattaaagg
     1021 tataagttat gttcctatat gcttaaaatc agtggaagta cagtatgcaa taacacttag
     1081 tattaaaaca tctttaatat ctactatagt atgcctttta ttagctattc cagtagcata
     1141 ttttttacat attactaaat tgccatttaa aaaactgatt atacagataa taaatctccc
     1201 tatgtctctt ccacacttag tatcaggaat tgctctttta cttttgtttg gaagaatggg
     1261 gattggagat tccatttata agatttttaa gcttgatttt atatttacaa agcaaggtat
     1321 agttttagca caaatatttg taaatcttcc acttactatt aaaatacttc acacttcatt
     1381 aaatgaaagt aatgaaaaga tgatatttgt tgcacgtacc cttggatgta atagttggga
     1441 agcatttaga tttataattc ttccaaatct aaaaactgga atcatatctg caactgtaat
     1501 gacttggtca agagcacttg gtgaatttgg tgcagtagct atgatagcag gttctacacg
     1561 tatgaaaact gaaattattc ctacatctat atatttaaat atgtctactg gtgatataga
     1621 cattgctatc ggaatagctg taattttgat atttctctca cttacatgct tgatgctttt
     1681 tgagatattt tttaatagag aggttgataa gaattaatat gttaaaaata aaaaatttat
     1741 ctcttgattt acctaatttt aaattacaag aaaatagttt aaatattaat aatcatgaat
     1801 actttgtttt attaggagct agtgggtcag gtaaaactct atttctagaa acattagcag
     1861 gaagatatcc atctgctaaa ggaaatgtat catacaaaga aaatttaata tcaaacttac
     1921 ctccagaaaa tagaaatata ggatttgtat atcaaaattt tgaattattt ccaaatatga
     1981 gtgttgaaga taatataagg tttccactta aattaagaaa aatacctata gatgaacaga
     2041 ttcttaaggc taaccacttg tctaaattaa tgcagataga agatataaaa aataggtatc
     2101 ctaaaaattt aagtggaggc gaaaagcaac gagttgcttt tgcacgtgcc ttaatatctg
     2161 aacctgatat attactttta gatgaaccta tgagttcatt ggattatatt acaaaaaaac
     2221 atataagcaa aattttgaag gatgtctaca taaaatacaa accaacagtg attcatgtta
     2281 ctcatgatat ttctgaagca atgttttttg ctcataagat aggaataatg aacaatggaa
     2341 aaataaatca tatttttgat ttaaatgaag aaataaaaaa taaaggagaa tcttttttct
     2401 atgaatatat ttgatatttt gagagattat ctattagtac aagtcgataa atataattta
     2461 aatatggata tgataagtat tgtgtccaaa tctctatctt caaaagaagc aattggaaat
     2521 actaagcgta aagatttccc aattatagtt ggtaaagaaa taatgcttga agctgacttt
     2581 aaaggagcta aaggacaagc atttacttct actccatcta catttgaagg ttctttaaaa
     2641 gacatattat cattagattt acatgacaat cctcatgata gaagtctttt tatagcatca
     2701 ttaaatgcag ttatgaaata cttaggcaat acggatagaa ccattcattg taaaaataac
     2761 gagccagagg tatgtgcaaa aaaattccct gagtttataa aaatggaatt tggtaatcca
     2821 aaagtagcaa ttataggata tcaaccagcc ataattgata atattaagga ctttttcgaa
     2881 acaagagtat tagatttaaa tcctgaattt gtggatacta ttcaatataa tgttaagatt
     2941 gaagatggta tacgagatta tgaagatgta atatcttggg ctgatttagt gatttgcaca
     3001 ggaagtacat tatgtaataa ctcaattata aattttctat cacttaacaa accagtttac
     3061 tattatggaa ctacgatagc tggtgcatca aatattttag gtttaaaaag actctgtttt
     3121 tgtagtaaat aagcaatcat atcatcaagg agtacacaaa tatatatgtg tactctttaa
     3181 atttaaaaag ttattacttt tattctaagt actaaagtaa aattctatta aaatgaatcc
     3241 cgaataaatg tacaatccct ttaaatctca attcctatca atcattaatg ttttgattag
     3301 tcttatacat aaatgttttc tttattttaa actaataaaa aaattttatt ccattaatct
     3361 actctatatt tttcagcatt taagaaataa tttttagtac tttgtttgat aatataacta
     3421 agacaacttt tcaggagatg attaaatgac atttaagaat aaaaaattta ctataaataa
     3481 atataaaatt ctatctatta ttctagtttt atgtttgatt ttaaccgctt ttagtagcta
     3541 tgtgaattat agtcataaaa aatttaaaga ttatgttgtc ttctatgttc agcatcaaga
     3601 tgatgaagtg ttgtgggctg ggagtgctat tgttaatgct ataaaagaaa gaggaaaaaa
     3661 tcatgtattt gtggttttag tatctactgg atgtggtata tccgtctttg ataaatataa
     3721 aaaatatgaa aacctaacaa atatacaaaa atcagaatat agaaatagag aatttttagc
     3781 ttctcttaat agccttggcg taataaggga aaatataata ttattaccag aaacaaatac
     3841 aaatggtaat actgacttca attacatgga aaaaattgct cttacatttg aagaaaaatt
     3901 taaaaatgta actcatatag cacatactta taagttagat gaccatttac aacatttaaa
     3961 aaatggttct gttatccaaa atttatataa tgctggtaaa ataaaagatg ctaaatattt
     4021 tgtaaagcct aaatttgctg ataaaataac ttttaataat aagatagttt ataaagctca
     4081 ttctactgaa gactatgaaa aggttaaaaa tgcctgtcaa caatacaaaa ttgttgatga
     4141 attagaatat agagaaggta ttggctataa gtctgaccat aaatcatttg ataagttatt
     4201 aaataataaa gatgttcctg ctatacttca tacacctaat ttataagaaa tatttattct
     4261 tttaatttaa gttataaaaa ggaaattatt tgagattttg tgatatagca ctttttgtgc
     4321 tatactactt tttctcatac aactattacc catatctaat ttcatcatct cttaaaatat
     4381 ataaagatat catcattaaa acctataagc cttattaaat gctttataca tatctacatt
     4441 aaactacctg tttatattaa actacattaa aataaatatt tgttataaat tcttaataat
     4501 tatgaaactc atttgttatt tatatttgtt aatatatgtt taaacctaaa aaattaataa
     4561 accaaaataa cataaggagt gtgatagatt accctagata ttttcataaa cttttacctt
     4621 cagattaata tgggcgtcta tacttttaaa tttactatat gcattgacaa tatattaaaa
     4681 gtactagttt ttatttcata atgaacgggc ttactgtttt ctaatacctc tttttggtat
     4741 aaaataaaaa ttggttaaaa ataaaatttc aagggggtta ataagttgaa tcaaaatata
     4801 ttggttgtag atgatgatag agagatagtt gatgctatag agttctattt gagacctgat
     4861 aactttaatg ttttaaaggc atatgatgga cttgaagcat tggacatact aatcgaaaat
     4921 gatattaagt taataataat ggatgtaatg atgcccaatt tagatgggct taaaactaca
     4981 cttaaaataa gagaaaaaag gaatatacca ataattctct tatctgcaaa atcagaagat
     5041 atggacaaaa ttataggtct taatatgggt gctgatgact atataactaa accatttaat
     5101 ccactagaac ttactgctcg agtaaaatct caacttagaa ggtatataaa tttaggtgat
     5161 tttagtaata caaataactc aaatgatact aattatattc taaaaagtgg tggcctagag
     5221 ctaaatacag atacaaaaat agtttcttta gatggtgagg aaattaaaat gacaccactt
     5281 gaatataaaa tacttacact tcttatgtca cgaaaaggta aaattttctc ttctcatgag
     5341 atttatgaaa atgtctggaa tgaggatgct tatagttgtg agagaactat tgctgttcat
     5401 attagaagaa ttagagaaaa gattgaaatt aatccaaaag aaccaaaata tttaaaggtg
     5461 gtgtggggca ttggttacaa aattgaaaaa cttaattatt aatgattcta aagcaaaatt
     5521 tattttatta ttattactgt ttctattagt ttctatatta tttgaagcag tttatgattt
     5581 gaataataac ttttcttata atattaattg ttattataaa gagtttgctc tcccttattt
     5641 ttattttttc ggtactcctg tacatttgtc aaaagtagaa ttagagtttg tctgcatagg
     5701 tattatatta tcaagttttt tattgactat actatgtttt acattgtata aaaaaaataa
     5761 acagactaaa atcaaattct tagagaatgt cataagcctt ttttctaagg taaaaataga
     5821 acttacactt atattaactt tgatactatt aaccttaagg tttcatgcat ttacaagtaa
     5881 taaaatgttt tacaacacta atttaataat ctacaat
//
LOCUS       DAEMHW010000209         5868 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEMHW010000209 DAEMHW010000000
VERSION     DAEMHW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.209"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(71..850)
                     /locus_tag="KPK67_003288"
     CDS             complement(71..850)
                     /locus_tag="KPK67_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2460210.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(905..1387)
                     /locus_tag="KPK67_003289"
     CDS             complement(905..1387)
                     /locus_tag="KPK67_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG2460211.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(1653..3074)
                     /locus_tag="KPK67_003290"
     CDS             complement(1653..3074)
                     /locus_tag="KPK67_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG2460212.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDVVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(3220..3990)
                     /locus_tag="KPK67_003291"
     CDS             complement(3220..3990)
                     /locus_tag="KPK67_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2460213.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELSNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(3995..5197)
                     /locus_tag="KPK67_003292"
     CDS             complement(3995..5197)
                     /locus_tag="KPK67_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG2460214.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT         1954 a         1006 c          566 g         2342 t
ORIGIN      
        1 ttatagatta aagattaatg atgtctgtaa tttaaactta caatcaccac taatcttcaa
       61 acaaatattt ttacttttgc acttgcttat ttttatctaa atgttgtata agttcagata
      121 ctgttacgaa tttatatcct ctctctttta atttaggaat tactacttct aaagcttgta
      181 tagtttgtga tttatcattt cttttagtgt tataatcatg taaaagtatt atattaccat
      241 tttggacctt tgtaaggata gtatttataa ttgcatctac tccagggtta gaccaatctc
      301 tagggtctaa gtttgaccaa agaattatat tcatgccttt agaaactata atatcacaga
      361 cacttttact catggctcta tatggaggtc taaatatctt tggttctact cctattacac
      421 tttttataat attttgcgta tctgttattt ctttttcaac actactataa ctattttttg
      481 caacatttat atgtgtaaat gtatgattcc ctatttcatg cccctcatca tattgtcttt
      541 ttaataattc tttgttatat tctacttttt ctccaactac aaaaaaagtt gcttttacat
      601 catttttctt taatatatca agtacttgtg gtgtaaaatc ctcatctgga ccatcatcaa
      661 aagtcaatgc taaaattttt tcatcctcat ttcctttttt tattattaca tcttcataat
      721 cgtcttttat attgtcattt aaactatatg cttcttttga actatcttga ttatgtgtat
      781 tatgattata tgtatataca cctactatag ataataaaca tatacatata ccccatatta
      841 ttttcttcat gtcattacct cttttctaat tcattctagc aatatatttt ataatgtcag
      901 ttttttaatc tctcaatatt ccatttttat ttaaatcagt taaatttgtt aggtcttctg
      961 aataaaagtt agaatcttct acatcagggt tttgcattgc atcacctaat aaattatttt
     1021 ctttctttgc acaatcatta cataaacttg tatctggcaa tatatctaat ctttctaaat
     1081 ctatttgatt atgacataaa tcacaaatcc catatgttcc attctctatt ttagataatg
     1141 ctaagtctat ttgatacaat cttccctctt catgatttat tagactgttt tgcatatttt
     1201 gcatatataa atctgttccc atatctccta tatgattatc ataactagat aattctcctg
     1261 atgaatactt ttcacttgta tgtttagttg tatctccaaa taaagtatta tctttcatat
     1321 cctctactaa attagttaaa ttttctcttt ctttcaataa tttatcttta tattttttag
     1381 tatccatttt taacacctct aattttaata tttatatcat aaaaatagta tgcggaaaag
     1441 taaattaaac atacaataaa aataagattt gattcgaaaa atggaatgtc tcaaaatgaa
     1501 atttttagtt catttaaccc tcttttttat atgaaatcaa atatattttt atcctttcag
     1561 caataaaaaa gagacttatc tctattttga gatagtctct tctttgaaca taatttaaat
     1621 ataatttttt aatttttata gttattattt tactaacttg ccaatttatg attattttgt
     1681 tctttaatat gttttgcttt tttaaagaat aaaagaccaa gaattattgc tacaataaca
     1741 cctattccat tagatatatt aactggtagt ctaaatcctt cttttgcttg cattatataa
     1801 ctaaatgtta ctacagtcat aaaaagtgct ggtaatgtag ttatataatg gtttttattt
     1861 gctttgacta aatatgcaga accagtccac aacattatca ttgcaagtgt ttgatttgcc
     1921 caagagaaat atctccaaat tatattaaaa tctataaatg taagagcaac accaactgcg
     1981 aataatggta aagctattaa aaatctattt ttgactacat cttgattata attcattgca
     2041 tctgctattg tcagtctagc acttctaaat gctgtatctc cagaagttat aggacatgcc
     2101 acaactccaa gtatagctaa agctcctcca ataggaccca ttaatccaac agatactgta
     2161 tttactactg ttgctgctcc accatcatta aatatcacat taagttgagg tatgccatgg
     2221 aaatatgaca tggctgctgc tgcccaaact aaagctatta taccttctgc aaccattgac
     2281 ccataaaata ctggtcttgc ctcactctct ttctcaacac atctcgccat aagtggtgat
     2341 tgagttgcat gaaaacctga tattgctcca catgctattg ttataaataa gtatgggaat
     2401 aaaggtgttc catttggatt catattttgt aaatttatat ttggtatgtt ataccccttt
     2461 attattaatc ctccagctac acctactgcc ataattaata atgctgctcc aaataaagga
     2521 tatattttac caatcaattt atctattggt aaaacagtag ccataatata ataaagtatt
     2581 atcactccaa cccatatttg tgcgtttaca cctgtcaatg tctttaataa tcctgctgga
     2641 cctgttacaa atacaactcc aaccaatact aaaagaataa cactaaatat tctcattgct
     2701 atttttgcac catttcctag ataaagtcct accaactctg atgcagatgt acctttgttt
     2761 ttcattgata aatatcctga agcaaaatcg tgtactccac cagcaaagat tgttccaaac
     2821 acaatccata aaaatgctga tggtccaaat aatgcccctt gtatagctcc aaatatcgga
     2881 cctaaccctg ctatatttag aaactgaatt aaaaagattc tcttccattt cattggcaca
     2941 taatctactc catccctaca agctatagct ggagtttgat ttttgtcatt gattccaaaa
     3001 accttttcta caaaagtacc atagacaaaa taacctacta taagtactac tattgaacct
     3061 aaaaagctta ccataaattc atccccttag tttttatata attgctttat aaatttttat
     3121 aagtttaaga aaactatttc cagttcaaat agtaatactt ttttatgcta atataaatat
     3181 taattacatt aagtgcatat ttaagtacat gaaatgactt tatatatgca ttaacagcct
     3241 aaattctttt actttgcttc tacttacagt tacttcatag tcactatttt ttaattttaa
     3301 tatgtatgta ttattaaacc atggtataat ttcttttact ttggttaagt ttacgatata
     3361 agacctatgt gttctaaaaa atgttttatc atttataaga ttttcgactt ctgatatgcc
     3421 ttcttttaat ataaactttt ctttctcagt atatataaat gtctgccttt cattagcttc
     3481 acagtaatat atatcatcaa tatctattac aactaattta tcacctttcc aaaggctaat
     3541 tttatgtgtt atttcttcta tctcctgatt tactgcttcc ttcttatatt tgtataaatt
     3601 actatttaca ttagatagtt caatatcact catctcactt ttttctaatt tattcagcat
     3661 agaaataatt ctctcttcag aatatggttt caatatataa tcaaatacat ccaaagaaaa
     3721 agcatctact gcataacttt catatgctgt tataaaaaca attttaggtc ttgatttaaa
     3781 ctgatttaat gtttttgcaa gtagcattcc atcaagatga ggtatattta tatctaaaaa
     3841 aattacatct attttatttt cctgaataaa gtctaacacc tctataccat tggtaaattc
     3901 acttactacc tcgattccac ttttattttc aataaaatat ctcaattcct ttcttgctgg
     3961 aaattcatct tccactatta tagctctcat acttctactc ctttacataa aatactatca
     4021 aagtcccatt atctattctt cttatattaa gccctcttcc atacatcagt ttaagcctta
     4081 aatgtacatt ataaagccca atcttatttt ctggcatatt atcttgatat acattattta
     4141 taatttcctc acttatacct ataccattat cctctattga tattctcaca gtattttcta
     4201 atgaatacgg taactttttt atggatattt tcactacacc agctctacca ctttctaaaa
     4261 taccatgtat tattgcattt tccactaatg gttgtataat caaacttgga atctttatat
     4321 ttacatcttc tatatcataa gatacagtta gtaattctcc aaacctagct ttttctatct
     4381 ctacaaaaga tttaacttgt tcaatctctt tatttatatc aattaagtta tcactatatt
     4441 caagattata tcttagataa gttgataaat ttattattaa ctctctagcc ttatctggac
     4501 taaatcttat aaatgaagct attgtgttta gggaattaaa caaaaaatgc ggattaattt
     4561 gtctttgaag tgcttttatt tctgctttat ttctcgcttc tatatctttt ttatctttct
     4621 caatcccctc tactatagat ataacaaatc ctattccaat ctgcccaact attaaaggta
     4681 aatatattcc accgattatt tgaattgcta aagaatgtgg atatgatatt aagtatataa
     4741 gtaatatagt aaaactttca gaaatcatgc ctaccaatat tccatacatt actctatgtt
     4801 gttttggtat cctcttatat agaaatcctg ataatacacc tgctagtata cttgataaaa
     4861 gacatggaac agatgttatc cccccaatat ccataaaata tctatgaatc cctgaaaaaa
     4921 ctcctgctgt aatacctacc attggaccaa ataaaatgcc acctgatact atagatatta
     4981 ttctagtgtt tactattgac cccatataat ttatcccact ataagttcca aatatggcta
     5041 aaaaagtaaa aactaatgct atacagacca agtcattaaa actatattct tccttttgaa
     5101 atatctgctt aaatattttt aactttgtta ttagaagaaa caatactaca ataaggcttg
     5161 tcttttctat taatgcgatt aaaattgatg ttttcataat ccccctttaa aaaataaatt
     5221 tttacacact ctaaatttat tatggttgtt ataaattaca tatttgaagt aatcctatca
     5281 caaaactttc cacatttaat cttttaatta aataagaaat agttgtactt ctattaacaa
     5341 ctaatactag ctagcataag cttgtaataa cttgtagtta ttattaactt tacaattcta
     5401 gtatttaatt ttatacttta gaaatctctt ttcttagaaa taatactttc gaatatcata
     5461 taatacaata ttatgtagat tcttactcat acaacatatt tctttaaaaa ttttatacta
     5521 ttctttgttc aaatatctca tttgatttaa ttgtgttaaa cacacattat catctctctg
     5581 ttatatattt attatatagc cgtatatcga taaagtaaat aaatcccata tttaaaataa
     5641 tatgtttgac aataaattat tataatgtat atttaccata ataatttgtc aaccttgaaa
     5701 gttacttcta taattattgt taaataattc ttctaaaaaa aatacctata aattttattt
     5761 tctgaaatac atagttataa ctatttttat cacgtataag aataaaaatt ccagaaaatt
     5821 tttattatct aagttaatat aaaacatatt atttaatata aataaatt
//
LOCUS       DAEMHW010000210         5805 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEMHW010000210 DAEMHW010000000
VERSION     DAEMHW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.210"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            472..693
                     /locus_tag="KPK67_003293"
     CDS             472..693
                     /locus_tag="KPK67_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBG2460215.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            1133..1363
                     /locus_tag="KPK67_003294"
     CDS             1133..1363
                     /locus_tag="KPK67_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBG2460216.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            1376..1525
                     /locus_tag="KPK67_003295"
     CDS             1376..1525
                     /locus_tag="KPK67_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBG2460217.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            1782..2219
                     /locus_tag="KPK67_003296"
     CDS             1782..2219
                     /locus_tag="KPK67_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG2460218.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            2476..3369
                     /locus_tag="KPK67_003297"
     CDS             2476..3369
                     /locus_tag="KPK67_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2460219.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVFNWLFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIGIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            3547..4371
                     /locus_tag="KPK67_003298"
     CDS             3547..4371
                     /locus_tag="KPK67_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2460220.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQPNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            4470..5078
                     /locus_tag="KPK67_003299"
     CDS             4470..5078
                     /locus_tag="KPK67_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2460221.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            5182..5358
                     /locus_tag="KPK67_003300"
     CDS             5182..5358
                     /locus_tag="KPK67_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460222.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
BASE COUNT         2242 a          684 c          749 g         2130 t
ORIGIN      
        1 ttagctttaa aattaatttt tgtattaact ttaataaaat ttctcataac ttataacttt
       61 gttagcttta ataaagattt taatggttta aaagtaaaat gttaatattg gacttttaac
      121 ttttttattt acagattaaa tttaataaaa tttttaacat aagctttaac atttaattta
      181 acataaactt tagtacctaa catttaactc tacacatatg aaaatccata caaaactatc
      241 tcaactaagc aggaatatat acaaatacta tttcaaaaca ctaagaacat atcttcacaa
      301 ataacttgta aactttttca tcatcaaaaa agtaaaattt ttcctataaa aatttacaca
      361 aaaaatcttt agaaattact gtttaactcc gatattatct tagatgatag tattatagct
      421 aacaaagaaa gtcgacaaat ctttgaaatt aaatttttag gaggtaattt tatggaagtt
      481 gtttcaacaa aaaatttatc tagtataaaa ctaaaattgg atgctggttt tgatgataag
      541 ggtaaggcag ttgttaaaag taaatcattt gcaaatgtaa aagcagaggc attaaatgaa
      601 gatgtttatg ctgttgcaga agcaatagca agtcttcaag agaaccctat tgtagatatt
      661 ttaaaattag atagtacatc attatctaaa tagtatcttt taaacgtact ctgctttaca
      721 aattaaaaaa attaactctt tatataaaag attaagttat ataacgcact taatacgcta
      781 tagatttggc agattataac ttcaattttg gttataactt taatatatta atgctttgaa
      841 aaattaatat attaaacgta tttcaactta aacatgttag ctattttaga tgctacccta
      901 ctttaatcta attaattgaa ataaattaaa tattttctat atcagcaata actagagtca
      961 acaattaaaa tactaatatt gacaattgaa acactgatac taacaagcaa aacactaata
     1021 ttgacaattg aaatactaat actaacaagc aaaacactaa tattgataat taaaatacca
     1081 atattaacaa gcaaacaatt aaaatataaa ttattatatc ggaggtaatt atatggaaat
     1141 taagaaaaaa ttagtcatga gctttaaaaa tgtaaaggat aaacaagttt ctttttctat
     1201 agaaaatcca aaggatgatt taactgaaga aaatataaaa tctgtcatgg atttgatagt
     1261 atctaaaaat attttttcgg ttggaggatt tgacctagct tctgttgttg aagccaaggt
     1321 tgtggaaaca aataccactc cttatgactt agttatagaa tagggggcta attttatgga
     1381 tgatagttta caactgttaa taaccaatgt tggttttcct atagctctat ccatgtactt
     1441 gttggttaga attgagggta aattaaatac tttatcaaac agtattgata cattatccaa
     1501 aaatattttg atatttaaga agtaatttga caatatatgt aagtcataaa ataggtctat
     1561 ttgtaaaata attagacata ttctatgtga aatactagga tatttggata atttatccaa
     1621 atatccatag tatttttttg aattttacca cctcaaattt gaatttcact gttttatttt
     1681 tgaatttcac tatgcttaat ttgaaattta gaacgcatct agttggactc aataagatat
     1741 aatgctattc taatgacagt aataaaatga ggtgatgatt aatgaaaata catgtcgaac
     1801 aaggtattaa ttgtgatgat attgaaataa ccataaaatg ttctgctcta gataataaca
     1861 ttgaaaaaat aatttcatta ttgagtacac cgttacttga tataaaaggt aaaaaatcag
     1921 gagagattta taaactcaat gtctgtgata ttttatactt tgagacagtt ggaaataaaa
     1981 catttgctta ttatgaagat gatgtatttg aaattgattt aaaaatatat aaattatcaa
     2041 aattattagc taatacatcc ttctcacgaa tatctaaatc catgatttta aatatggact
     2101 acataaaaag tataaaagta atatcacata atcgcatgca agctacttta aaaaacaatg
     2161 aaaaagttat tataaatcgt cgttatataa aaaatattaa agaaaagcta aacatttaat
     2221 aaaaatctaa agtaatttat taatatcact aattacaaat ttattgacct atttaataat
     2281 taataataat ataatatgtt caatattaaa tactaattaa ttatatttag tataaaaata
     2341 attaatcaaa atgtaaaaaa taggatatta ttataggcaa taactaacat caagcgataa
     2401 cttaatacat ataaaaataa ataaacaatt aataaaaatt aaaacttcat tataaaacgg
     2461 aaggatagtg aaaaaatgca actatataac acccccttta atacaaacaa acacttaatc
     2521 tatttcttta catttacgct agtatttaac tggttattca gtcttacacc tgcgttgctc
     2581 ggaattacaa atacaatact tggagaaatc attttttatc ttgcatatac atcaccagct
     2641 attgtaggtc tattttttat ttctacaatc tatccacaaa gtgcaaaaca agattttatt
     2701 tatagatacc taagctttaa agaaatcgga ataaaatgga tactattaac aatattattt
     2761 tttgctttaa cttttattac ttcgttaata atcggaaatt actttaatgt aaaaacttct
     2821 gatatagatt ggatacacat agcagtgttt agaccttcta aaatagctta tatgctatta
     2881 gcaagtttta tattgagtat acttagtcaa gaagctggat ggagaggata cgccattgac
     2941 aaattattag ttagatttgg atttataggt tcttcaatta ttttagggct tgtttgtgga
     3001 atctggtatc tagggtcgta ttttacacct aatcaaatac catataattt ggcacaatac
     3061 tccctatttg atgctttttt atttatacca aacattgtac ttttaaactt tataattaat
     3121 tttgtatata tcaatacaag tagaagcatt ttatcagctg gattagttca tatgatgtac
     3181 tattttttca acattcaatt acttctacac taccctatta aattatgtgt aatagctcaa
     3241 tatgtacaaa taatatttgg tttaatattt cttgtatata tgatttcttc taataaattt
     3301 aaacagaagg taaattctga aattgaacag ataaagtcgg atgattttga atttgagtta
     3361 gactggtaat tttaatatga attataaatt aagataatct atgatttgat acgattacta
     3421 cttaaatata atttactctc tataatttat tgcaacatct ggaaaataat tgtatttcca
     3481 caaacgcttg aagtatataa aaacaaaata tatactgatt ataagtgaaa aggaggtcat
     3541 attttcatgt ttaaaatagg agaggtttca aaactaacac aaatttctat tcgtatgttg
     3601 cgttattatg atgaacttgg tattttaaaa cctgcagaaa tagacaaata tacaggtcat
     3661 agattatact cagttgaaca gatttcaata ttacaaagaa ttgtattatt gagagattca
     3721 aaatttagcg ttgcagaaat agcaaatatc gtacataatt ggaatgatga atttgtaatt
     3781 aaggaattga atagaaaaaa gaatgaaata caaaaggaaa taaagcagga acaacaaaga
     3841 ataaataaaa ttgataaatt tatagaagct ataaactgtg ataaagatga aattcattac
     3901 aatgttgtct ttaaaaagat tccaagttat aaaatcatat cacttagaga gataattcca
     3961 gactaccaat ctgaaggtat actctgggaa aagctatcta aatttatcaa agaagaacat
     4021 atagaagtat cacgtcaacc caacaataac atagcttttt accatgatga agaagtcaaa
     4081 gataatggtg tagatattga agttggaatg gttgtaaaga agattggaaa gaacaaatct
     4141 ggatttatat atcgtgagac agaagaaatt gatatgatgg catgtactat ggtttatggt
     4201 ccatatgaaa atatagctgg tgcatatgaa tcgttctgtt attggttaga taaaaatagt
     4261 gattatcaaa tatcaggtat aaatagacaa atcggtcata aaggtgaaca taatgaaata
     4321 aatcctgaaa attatcttac agaaattcaa atccccctta taaaagtcta atcgattagt
     4381 acttaaatac ttaagtacta atcacccatt gacatcccaa aattttcatg atagtatcta
     4441 aacgtgtatt aaacatgttt aattttttta tggagaaact tttaagggga aaaattatga
     4501 aagcagattc aacaaaggaa caattaatta atgccacaat ccagttacta tcttcacata
     4561 gagatgtttc aagcattacg gctagagaaa ttgtagctaa agcaaatgtc aatttagcta
     4621 tgattaatta ttattttaaa tctaaagagg agttaattaa tatttctatt aaaaaatttt
     4681 taaagcgtca tatggatgaa tataaggcta cacatgatac taaaaattta gcacctaaac
     4741 agcaattaag ggaaatacta attgcatttt ttgattttat aggtgaacat agtcaattta
     4801 taaagatttc tgtaccctat atattgatac aggaagaaat tgtttatcca tttgagatac
     4861 tacctttaat taaggctcac tatgaaaatc agaataacaa aaaaactgaa gctgaataca
     4921 gaactattgc gtatcaaata acttcattta ttcagttgct ttttcttcgt tctaatgctt
     4981 ttttcaagta tagtggtatt gatattctta atgttgaaca gagaaatggt ttaatagatt
     5041 ttcaattaga tttattattg gtagatatta caaaataata ctatattaat tttttatgta
     5101 cattttttta ttttatggta acgtttgaaa atattctcat tgactaaaaa tacttgcatt
     5161 aaataagggg gaggaatttt aatgtttttt acatttatag gagtaatatc agcaatttta
     5221 ttgttgtgta caattatatg tggtttatgg ataaaactta ataatcgtaa taatgactct
     5281 aattctcact ttactttttc aatactttca attgcttttt cactgttaat tgtggtggcc
     5341 tttttagtaa gaaattaatg tctttgttat gctggggttt attattatag atttttatta
     5401 ttgcatgatt tttactatat tatagtattt ttattatatt acagggtctt atttattata
     5461 gtgttttgtt gttacagcat tttattatat tacagagtcc tatttattat aggattttgt
     5521 tatattacaa tctcttattt attgcagtgt tttgttatta cagtatttct attatattgc
     5581 aaactcttat ttattatggt attttgttat tgcaacattt ctattatatt gtaaagtctt
     5641 atttattaca gttctttatt atactacaaa cctttattat tacagcattt ctattacatt
     5701 gtaaagtctt atttattaca gttctttatt acactacaga cttttattat tacaatctct
     5761 tatttattac agttctttgc tatactacag acctttatta ttaca
//
LOCUS       DAEMHW010000211         5731 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEMHW010000211 DAEMHW010000000
VERSION     DAEMHW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.211"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            309..1490
                     /locus_tag="KPK67_003301"
     CDS             309..1490
                     /locus_tag="KPK67_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2460223.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1632..2549)
                     /locus_tag="KPK67_003302"
     CDS             complement(1632..2549)
                     /locus_tag="KPK67_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460224.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2802..3596
                     /locus_tag="KPK67_003303"
     CDS             2802..3596
                     /locus_tag="KPK67_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460225.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            3597..5657
                     /locus_tag="KPK67_003304"
     CDS             3597..5657
                     /locus_tag="KPK67_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG2460226.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMSFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLKVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2195 a          641 c          935 g         1960 t
ORIGIN      
        1 taagggagtg tctcaaaata aactttttag ctcatgagac acttttttta tatgaaatca
       61 aatatatttt cattgtttag atttattttg ataatgaaaa aaagattatc tttattttaa
      121 gaaagacatt tatttataat tatatacaaa aatatttttt aatgtttttg tgtataatta
      181 taacaattta atttatatta atgcacttaa atttgaaata gaatgcttat agtggtaaaa
      241 ttaattatta gaatattaga aataataaat aaataataca ttgtacttaa aagggggaga
      301 aatttaagat gaacaaaaaa tatattaaag cagggaattt aattgatgga accggaaata
      361 gtattatgca aaataagggt ataattattg atggagatac tatagttgaa attgaagata
      421 taagagatga tttaaatgga tatgatgtgt acgattactt agataaaact gttatgccag
      481 gcattatgaa ttgtcatgtt cacttgacaa tggagccagt tggaaatcca aagatatatt
      541 atgataatgt atcagatgta gagcttgtag taaatacgat aaaacaactt gatgcttatt
      601 tggattcagg agtaacatat attagaagct taggttgtcc caaatatgtg gatgtacaac
      661 ttaaaaatct tatagacaaa gggtctatag atggtcctgg tatagttact tcaggtccag
      721 ttatatgtat gacaggaggg catggatatt attttggtat tgaaagtgat ggagtggatg
      781 aatgtagaaa atcagccaga acagttttaa aaaatggagt agattgtgta aagataatgg
      841 caactggagg tgtaacaact gatggagttg agccagggtc accacaatta actttggagg
      901 aaatgaaggc tgctgtagat gaagctataa aagcaggtaa aataactgcc acacatgcac
      961 aaggaagaac tggtataaaa aatgcagtgc ttgcaggaat tacttctata gaacatggtg
     1021 tttatttaga tgatgaaata attgatttga tgcttgaaag aggaacttac ttagttccaa
     1081 ctgtggctgc accatatttt attctaaatg caggaaaaga aagtggaata actgaagcta
     1141 ctcttagaaa gtgtgagatt gttgccattc cacacaaaga gtcattccta aaagcatata
     1201 aaaaaggaat taaaattgca gttggttctg atgcaggaac ttcttataat gaacatggaa
     1261 aaacatatta tgaaatgaaa ctaatgtcag attatggtat ggataatatg gatataatag
     1321 ttgctgctac aaaaacagca tctgaacttt taagaattga taaaaattat ggaactctag
     1381 aaaaaggaaa gaaagcagat gttatagttg taaatggaaa tccagtagag aatatagacg
     1441 tacttgcaga tgtgttagca gtatttaaac ttggtaaaaa agttagataa atagtataaa
     1501 aataaaaaag agtgtctaaa gaaattttta aataattata gacactcttt tttattagta
     1561 ataattaaat cttttatact ttagaaaata gattctattt tatatttaga tataactctt
     1621 taaaatcatt cttatgcaat ataatctata gaatctacac atacaaagta tattcttgaa
     1681 tcattagaat ttccaagtac tataacatca tttttcttac ctagaacttc aacaccagtt
     1741 agaaccaaac taccagctac tacagtaact tgattattta tatttgacat ataaagatta
     1801 tctaggatac ctttattaca acagcaatca tcacaatcgc agttgcaatt acattctttg
     1861 taatgttttt tatcaagata gtaacctaaa gatgctatga aatcttcata atctgcaggt
     1921 gtaatatcaa atgcaacagc tttaagatta cacaatgatg ctaaatctac gtttgctaat
     1981 ggagtagatg cagcaagtat aaaatcaatt atagcagcaa gtattgcatc taatatagcc
     2041 ccaaggtcta tcgtaggagg aataactgca tcaataagtg caattaattc agctactcct
     2101 ggtatagttc ctatagtatt aattaatccc tcaagagtta aagggactgg aataggataa
     2161 gatactctac cagctatatc tattaaatca cagttacaag cagaaaatct ttcaaaagta
     2221 ccatcaagtc cagataaatt atcttttgga ggagctgaaa gatctatacc taccaaatta
     2281 gcacctacta taaagaaatc tgagataaaa gcaaattgat taaagtttac aaaaggtctt
     2341 agagcatcat atcttagaag ttctaaggcc tctctcatac tttttttaca acaacattgt
     2401 tttattttag gttctttata tttatttgga taattatagt atctatcagc tacttgataa
     2461 ttttcatagt atctttcatt tgtatttgga taatcttcct catattcttg tgtaaatcta
     2521 aagtcctctc tacatttatt attttccact aaatacactc cttttatatt ttaatttaaa
     2581 tgtatagata attaagctta tctatatagt aatattatat ttataatgta aaaaaatgtt
     2641 acaaaagata ttataaacga ataaaaaata ataatttgtc agataaactt gtaaattttt
     2701 atgaataatc cattctaaaa caaaaaactg tgtataaaaa aggtttatat gttatattta
     2761 tataaatata acatatgaac gagaaaggag tggtttgata aatgattgag gtagataaat
     2821 tgtgtaagtc ttttacaagg gttgtaaaag atgataagaa taaaagttca ataagaaagc
     2881 taaagaaagt aaaaactaaa aaagaagaat tttttgctgt aaataatgtt tcatttaaag
     2941 ttatggaagg tgaaattgtt ggaatattag gtccaaatgg tgcaggaaag actacattac
     3001 ttagaatgtt aggtggcata ttaacaccaa catcaggaag tataaatatt tctggatatg
     3061 attattcaat agatagaaat tcagcaaaaa aagaaatagg ttatctctct ggaaatacaa
     3121 aattatatgg aaggctttct ccgagagaac ttctaacaac ttttgcaagt ctatatgaga
     3181 tgtcaaaaga agatatagaa gaatcaattg aaaatattgt aaaaataatg gatatgagtg
     3241 aatttataga taatagaatt gaaaatctat caactggtca gactcaaaga acatctatag
     3301 ctcgttgttt aatacattct ccaaaagtat atatttttga tgagccaaca ctaggtctgg
     3361 atgttttaag tagtgcatct attatagatt ttatgaagaa tgaaaaacta aggggaaaaa
     3421 ctgttcttta ctcaactcat tatatggaag aagcagaaac tttatgtgac aagattttta
     3481 tgattcacaa tgggaaaata attgcatcag gaacaccaca gagtttaaaa gaggaatctg
     3541 gagtaaataa tttgagagat gtattcataa aacttgccag aaaggaggac atttaaatga
     3601 gaagtaagat tgtaaaatac atatttaaaa aagaaatgtt agacatattg agagataaaa
     3661 agactttatt tatgatgatt gttgtgccac ttcttttata tcctttactt atgttattaa
     3721 tgattcaagt aatgaatatg tctgcaagta gtatgaatag taaggacatt aatatagctt
     3781 ttaataataa acctaataag gtgttagtat ccatgattga agatgataat ttagaaaaag
     3841 atactaaaaa tacttcaaat gataaaggca aaataagagt gttagatgta gataactata
     3901 aaaagtcatt agaagaaaat aaaattgatg cttatataaa gatggaagaa aaaaaatcat
     3961 ctataaatta tcaaatatat ataaattctt ctcaagaaaa ttcttctaat gcactaaaaa
     4021 gaattgaaac tgttttggat aaatataaaa gatttaccat agaaaaaact ttatctgaaa
     4081 aaggattaga tgtacaagct actttagaac caataacata tagcactgta gatgttgcaa
     4141 agacggaaga agtggcagga tactttttag gtcagatact tccatttata ttaataatag
     4201 gagttttact aggttcaata tatccagcaa tagatgttat ggctggagaa aaggaaagag
     4261 gtacattaga aacattattt acactaccaa tatcaaactt agaactggtt atgggaaaat
     4321 atatggcagt ttctcttagt gcaatagtaa cagcaatttt aaacatttta tctatgggac
     4381 tgactatgtc atttatttta gttagtggag gtattagtag ccaatttggc tttggaaact
     4441 ttaattatgg agacttagtc ttaccaatta taactacttt aatttgcata tgtctttttt
     4501 caatggtagt ttcagcaata agtatgtgtg tatgttcatt agcaaaaagt tttaaagatg
     4561 ctcaaaacta cataacacct cttatgttaa ttattatgat accatcatat gtttctatga
     4621 taccaaatat agtattagat agagtaacag ctgtaatacc tgtagtaaat atatctttac
     4681 ttataaaaag tgtattatct tttaaaagtg atttaaattt aatagcttta gttctggcat
     4741 caaatgtagc ctttgttata ttggcaatca tactactgtc taagatgttc aattctgagg
     4801 aaatactttt tggtaatagt aagagtttct ctttcttaga gaaaagaagt aatataaaaa
     4861 aagggtctat accaactgta agtgatggtg ttattttata tgcagtagga ttagttctat
     4921 tgatttatgt tggttcttta gttcaaatta aatttggtat ggcaggtatt ataatgacac
     4981 aattcatgat aatttcttta ccgttattat ttgcatacta tattaaagct gattttaaag
     5041 aggtatttag cttgaaagtt cctaaattaa agcatgtgtt tgggagcatt gttctttgga
     5101 ttggtggata catacttatt atgattataa cacagctaat tttattctta tttcctcaaa
     5161 atatggaagt tgctgaaagt ttaaataagg ctctttttat caaagatagt gttttactga
     5221 atttactagc aatagcttta ttaccagcta tatgcgaaga aatgtttttt aggggattta
     5281 tattttcttc atttagtaaa tcaaaagata aaaataagtc tatcaagcta gctataatat
     5341 gcagtggggt actatttggt attatgcata tggattttat tagaataatt ccaacatcaa
     5401 tattaggtat tatatttgca tatagtgtgt ataaatctgg ttcaatattt gtatcaatgt
     5461 tattgcattt tttaaataat agtgtagcag ttgtgttaaa tcattataca actggaaata
     5521 taaccaactt atataaattt gttgaagttg atttttcgaa cttaaatgtg cttaaatttg
     5581 ccttaattct tctaataagt cttatattaa ttatgttagg attaagagtt ttagatagaa
     5641 agtctttaag aaattaagta taaagttatt atatagatta aaatattaat aagcactaaa
     5701 gtatatggat atatcttgta tataaaaata c
//
LOCUS       DAEMHW010000212         5679 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEMHW010000212 DAEMHW010000000
VERSION     DAEMHW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.212"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            73..1185
                     /locus_tag="KPK67_003305"
     CDS             73..1185
                     /locus_tag="KPK67_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2460227.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1393..1869)
                     /locus_tag="KPK67_003306"
     CDS             complement(1393..1869)
                     /locus_tag="KPK67_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460228.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2067..3692)
                     /locus_tag="KPK67_003307"
     CDS             complement(2067..3692)
                     /locus_tag="KPK67_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2460229.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            complement(4030..5049)
                     /locus_tag="KPK67_003308"
     CDS             complement(4030..5049)
                     /locus_tag="KPK67_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBG2460230.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            complement(5046..5615)
                     /gene="mocA"
                     /locus_tag="KPK67_003309"
     CDS             complement(5046..5615)
                     /gene="mocA"
                     /locus_tag="KPK67_003309"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBG2460231.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
BASE COUNT         1932 a          964 c          589 g         2194 t
ORIGIN      
        1 tctatttcaa aattattttg tgatatatat atgttataat aaatgtataa aattttgatt
       61 ggtgggttat atatgaatgc atttattaga aaaagaaata aaaactatgt tgtttacctg
      121 gaatttgaag atgaaaagac aggtaaaaga aaacaaaaaa atatgggtac ttttgataaa
      181 aaaagggaag caagtaaaag actaaatgag gtaaaagaaa gcttatataa tgatggtttt
      241 ttagtaccaa gcgaaattac agtgtcagaa tttctattag attttctaaa taaatatagt
      301 gaaaatatat ctttagctac atataataat tatgttagta tttgtaagaa ttacattaat
      361 ccatctattg gaaaatataa aatacaagac ttacatccgg ttcacataca aaactacata
      421 gataaactat cttataaact aaatcctcaa tcaattaaga ttcatataaa tatattgaaa
      481 cttgcaataa aaagagctta tagactcaaa ttaataaaag aaaatgttat ggatagtata
      541 gaggctccaa gatataaaaa atttaaaaat gagatttatg atagagaaca aatgataaaa
      601 ttattagatt tagcaaaaaa tacagaaatg gaattaccta ttaatttagc tattggatta
      661 ggtcttagaa tttcagaagt tttaggtcta acttgggata atattgattt tgaagaaaat
      721 acaataacag taaataagat aacttcccga attaatggtt ctgttattct caaagagcca
      781 aagacagaaa gctctgttag aaaaatatct tctcccaaag aattaatgtc tttattaaaa
      841 gaatataaaa taaaacagaa taaaaattta ttaaaatctt ccattagaaa taataataat
      901 ttgttgtttt tcaataagaa atgtgaacct attgctgaag atgttatgag taaaaaattt
      961 aaaagatttt tagaaaagaa tgaattacct catattagat tccatgattt aagacactca
     1021 catgttactt tacttataaa ttctaaagta cctataaagg ttatatccga gagagtagga
     1081 cactcaaata taagtactac tctaagcgtt tattctcatg tacttaaaga aatggataaa
     1141 gaggcttctg acaaaatttc tgaaagttta tttaatgcta attaatatta atttatctta
     1201 gctttaaatt agcttagctt attgagatag attcattaat attaaatgta tctatctcaa
     1261 attaataact acttaaataa tctgttttat tttaatttat cgcctcagtt attatacttt
     1321 tttacgaaaa attatacttt acaataaaaa agatattccc ttatagaaaa tatcttttta
     1381 aatactatat tattatatac ttgctaacat tggaatatct aacatcttct tcattacatc
     1441 atctactttt tttgctttat attcattgta tccaattaaa tatccttttt catatgaact
     1501 ttttaaaatc tctttttcat ataaattatc agatttggct tctgtgacac ttaaattata
     1561 aagcccttta cacccatctt cataaccttt attataccct ttttctgcaa aatagttttt
     1621 atgatttttt tcgttatgta ctgaagatat tgttactcca gaattttctt ttgtactagc
     1681 ataaggacaa aaacctttat tgtgttgatg tgctgaatgt cctccacaat aataacgtgt
     1741 taactctgat ttatttttat tatgatgacc tcctgatgaa ttatgccctc ctgagtgagc
     1801 atatgaaaaa ttccctacac taatcatagc taaactcaat actacagcta ctaacctttt
     1861 cttaaccata atctattccc ccaaaaatgt tatatatttt tattatatca tctactatat
     1921 tatatttaaa ttcttaatat acaaaatatt actctattct gctaataaac atgtttaaac
     1981 aacataaaaa cctcctaata tttgattaaa aatttacaca ctcatgcata gcatgtctca
     2041 aatattaaaa ggttttatac taataattat ttatcttttc catacatttt agctaattgg
     2101 tcttgtatct ctttactttc taaatactca tcaaagtcca tttttctatc cattattcca
     2161 tttggagtta tctctataat tctatttgct atagtagata taaattcatg gtcatgagaa
     2221 gtaaataaaa gcactcctgg gaacttttca agtcctttat ttacagaagt tatactttct
     2281 aggtctaagt ggttagtagg gtcatctaat acaagtacat ttgcatttga aagcattagc
     2341 ttactaagca tacatctaac cttttctcct ccagatataa cttgagcttc ttttaatgct
     2401 tcttcacctg agaataacat tctacctaaa aatcctctta taaaactttc acttttttct
     2461 tcagagaact gtctaagcca gtcaaccaaa gaatattcta ctccgtcaaa aaacttatta
     2521 tgattttttg gaagataatc ttgagaagta gttactcccc atttatactc tccactgtct
     2581 ggttcaagtt ctcccataac tatattgaaa agtgcagtag tagctatttc atctcccata
     2641 aatactactt tatcatctct atccaatcta aatgacacat tatcaagaac cttaacccca
     2701 tctatagttt tagttaaatt gtgtacttct aaaacttcat tcccaatttc tcttgcaggt
     2761 gtaaatccta catatggata tcttcttctt gatggttgta tatcttcaac ttctaatttc
     2821 tctaactgtt tctttcttga tgttgcttgt ttagcctttg aagcattaga actaaatcta
     2881 gctataaatt cctttaattg agctatcttt tcttcagtct ttttattttg gtccttagcc
     2941 aattgtaaag ccaattgact tgactcatac cagaaatcat agtttccaac gtacatttgt
     3001 attttaccaa aatctacgtc aactatatta gtacatattt gatttaggaa atgtctatca
     3061 tgtgatacta ttataactat agattcttct aaatccatta taaaattatt taaccagttt
     3121 attgatttaa aatctaagtg gtttgtaggt tcgtccatta atagtatttc aggcttacca
     3181 aaaagtgatt gtgctaacaa taccttaacc ttttccccac caactagttc cttcatttgt
     3241 ttgtaatgca tatctttcgt tatgccaaga cccataagta ttttctcagc attagtttct
     3301 gcatcccatc cgtcaagctc tgcaaactca ccttcaagtt cggcagcttt tatcccatct
     3361 tcttcattaa agtcttcctt catatacaat gcatcttttt ctttcattat gttccaaagt
     3421 ctctcatgac ctcttataac aacatttaaa acagtttctt cttcatattc aaagtggtcc
     3481 tgttttaaaa ctgacattct ttctttatca gttatagata tactacctgt attaggctct
     3541 atttctcctg ataatatttt taaaaaagta gattttcccg caccattagc tcctattatt
     3601 ccatagcaat tacctttagt gaattttaag ttaacatctt taaatagttc cttatcacca
     3661 aatctaagtc caacacctgt aacttgtaac attaatatat tttcctttct ttttgctcat
     3721 aatttatttt atcataaaac tttgataaca tttttattat atattatcac gtaaatagta
     3781 tattttttat actaaatttg tgaaatattt attaaaataa tactaaaaat tgtaaattta
     3841 taaaaattaa caatctctta tattatactt ataaagaaat caaattgcaa ctttattcat
     3901 tcttttacac attgaaaata agttattata atttctaaat aaatatgctt tttttaaaac
     3961 atcattctag ctgttttgtt tcatatttaa aaagagtact taatttgtaa atactctttt
     4021 ttaataactt tatattttag caaaattaca tgcaggataa gtacatacat cacagttcat
     4081 acaaaggcca ccatgaccat atctagctat atcatactta tcaagctttt catcaattaa
     4141 aaccctagat aaaactatat caaatactgt agttttgtga tacattgcgc atcctggtat
     4201 acccataatt ggaacttctc cataataagc aagtaaaaac atagctcctg gtaatattgg
     4261 tgaaccatat gtcactaaat cagctccagt ttcttttata gctgttggcg taacatcatc
     4321 tgggtctaca ctcatcccac cagtacaaca gataagttct gctccttgac ttataaattc
     4381 ttttattgca ctctttataa tctctttatc atctgggcat atagtctgtc ctataacttc
     4441 acaaccaaag ccttttactt tttcttctat tacgggtcca aatttatcaa caattctgct
     4501 ataaaacact tcatttcctg tagtaacaat tcctactttc ttaggtttaa aaggtactac
     4561 atttactatt tttctattac caactacttt ttttgcatct tctaattttt tctcatctat
     4621 aaccaaaggt atcactcttg ttccagctac ttttaaacct ttatttacta taaaattatt
     4681 atgaagagta gccatcatta tttctccaac acaattcaaa tcaaaaagtg catcaacatc
     4741 tatctttaat agaccatcac atgctgctat aaaatctatt ttcccttctt ttatttcact
     4801 aaaatctaaa ttttctccag cagttagttc ttttaaaaac aatgcgcctt cattttcatg
     4861 aaccattcct tctgactttt cccatacata caaattatct ttaccaagag ataataacac
     4921 tggaatatct tcttctttta ctatgtggcc ctttttaaat tttcttcctt taaattctcc
     4981 aggtattatt tgagttatgt cgtgacataa aacttgtcca acagcatcta tagtttttat
     5041 tagcttcata atttaccccc aattctctca tagtccattt ttgtatcgat atcaaataac
     5101 atttctttag gaacttctac atactttatt tttgatgaat tttttaaaat ggttttacct
     5161 ttttcatctt ctttaagtgc caacaattct tctttcttgc tatatgggaa tatgactgga
     5221 tttccacaca tatctttgta tcttggaatt actataaaat ctctatctaa tttaaaagta
     5281 tttataattt tatctatata taagttatca ataaatggct ggtctcctac aaagaacata
     5341 taaccatcac attttgagct atttaaaact cctaatttta tagattcact ttgacctata
     5401 tttgcatttt tattatctat atattcaaaa ccatatttat tacacaataa caaaacctct
     5461 tcatattgac ttactactac aacttgatga aaatcaatct tgctaacttc tctaaataca
     5521 tgttctatta tagaacagcc tttataattt aataaaagtt tatttgctcc cattcttctg
     5581 gcaaagcctg atgccattat cactgcattt atcatatata tcatccttgg tatttataat
     5641 atattgatag ttctatgatt tttataatat ctctattga
//
LOCUS       DAEMHW010000213         5653 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEMHW010000213 DAEMHW010000000
VERSION     DAEMHW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.213"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..298)
                     /locus_tag="KPK67_003310"
     CDS             complement(<1..298)
                     /locus_tag="KPK67_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460232.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRH"
     gene            complement(654..1139)
                     /locus_tag="KPK67_003311"
     CDS             complement(654..1139)
                     /locus_tag="KPK67_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG2460233.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRKDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     regulatory      complement(1222..1315)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1671..2054)
                     /locus_tag="KPK67_003312"
                     /pseudo
     CDS             complement(1671..2054)
                     /locus_tag="KPK67_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      2280..2377
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2432..2608
                     /locus_tag="KPK67_003313"
     CDS             2432..2608
                     /locus_tag="KPK67_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460234.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGESVVE
                     SCLHQVMVLVSKYN"
     gene            2834..3034
                     /locus_tag="KPK67_003314"
     CDS             2834..3034
                     /locus_tag="KPK67_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG2460235.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     repeat_region   3398..3622
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3463..3491
                     /rpt_unit_seq="ctttacataccacatagttgatataaacc"
     gene            3849..4031
                     /locus_tag="KPK67_003315"
     CDS             3849..4031
                     /locus_tag="KPK67_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBG2460236.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIMF"
     gene            4102..4946
                     /locus_tag="KPK67_003316"
                     /pseudo
     CDS             4102..4946
                     /locus_tag="KPK67_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5170..5334)
                     /locus_tag="KPK67_003317"
     CDS             complement(5170..5334)
                     /locus_tag="KPK67_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460237.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         2052 a          735 c          596 g         2270 t
ORIGIN      
        1 tatgtcttct tgcgttttgt cttttatatt tatttatagt ttgatatcca tactttttag
       61 ttcttatact tcgttttttc aactttactt tacttttatt atatttccta cttttatgtc
      121 tacttttaga tttaatagtt ttaaatttag tattatcata tgtataattc tcttttattg
      181 aagaaatgta tacactagac tcaataatag cttctgcttc tacttgtaat ggatttataa
      241 tgctgattat aagtagaaaa gatatagcta tcccaatcaa ttttttcttc ttattcatat
      301 tttccccctc ataaaaattt ttaactagtt cattataata tatatataac taaaaattac
      361 aaattttttc aagtttctta tatatcttat tatctttaat attattttta ttatcataac
      421 ttttactttt tttaagattt ttcaaagtag tattaattta tgatacttgg tgtataaagt
      481 ataagttaaa tctactttca attaaattta ccatttataa ttactttata ataaaaataa
      541 attagttaat ttattctctt tagttctgtt ataatgtttc tacaaaaaag aatcccatta
      601 atgggattct tttttgtgat ttattaatat atttaaagct tgctagtatc gtattataaa
      661 cttaaatgct cttctatgta ttgattatat acatatccag aatatcgttt ttcattttta
      721 ttgatattaa ctcttgccca ttttccaacg ctataacata cttcaacttt aaaaccatca
      781 gggattgtag ttataacttt agattctaat gttctatcct ttctaagttc taaacttaat
      841 ccatttgttc ttatatttcc tatgtaattc ttttttctat tttttattgt atcattacta
      901 ttgtttttag cattacttaa tctttctaaa tatttataaa gtaaccagcc tttgtcataa
      961 ttactctcaa cttctgccca tattccatct acacacttta acttaacaat tttaccttct
     1021 ggtatacttt ttaatttcaa actatttatg tctggttttt ctcttaaatt caatttaatt
     1081 ttagtcttaa aattgatgct agatttgcga aaatttttta acattaattc agatatcatc
     1141 ttaagccctc ctcttgccaa atattaagtt attaaaatat ataactctaa aaatactttt
     1201 aacaaaaaga attattatct ttttggcaac tggctgacat gacctttatt acataaagat
     1261 tttagtccct atagttttgc gtcattagat ttatctaata ttgcctatat ctgaataata
     1321 acataatttg aacaattaaa aaataatatt acctcaaatt ttatattatt taaatataaa
     1381 ttaaattttt aattaataat aatcaaaaaa tatctttata tttattcaat tactgttctt
     1441 tatagtcaat ataaaagaca gtaatttatc tatagtgtta cttatatttg atatatatgg
     1501 ctatttttca acattttaca atatatataa catttatttt tttaactttt gtcagtaaaa
     1561 tgtcagcaaa acactttatc cacaattttt atataagttt tccacaactt atccccaaca
     1621 ttatccacat aaaaaaggta gctcttatat tattagctac cttttattta ttaatcttca
     1681 tatttccagc tttttatcca acttcctatt tctattattt tttcttcagt tatttcttct
     1741 ccatcatgta attttgaaat taattctgat aaaggattgt tatgtaaacc tctaaaaact
     1801 ctttttccct ctattttgag atattctctc tcttttatta atactgtata gtgtacatgc
     1861 ctcccaattc tctatgatat taaaaaacct tttttggtta atctagctaa aagtataatt
     1921 gtagttgtat gcttccaacc atatgttttt tctatttcat ctgcaacatc ttttgatatt
     1981 acagttttat aaccagtatt ccatatgcat ttcataattt ttaattctgt atctggtaat
     2041 tttttattta gcataatata ttccttcttt caatttatgt cacttcttca aatttatcta
     2101 gcttaaatta taataggagc tttttagaac aaaaactata tttaattttt accaggtact
     2161 ctacgtgttt agtctgtttt taagtgtaaa ataatgttca tatctataaa tttatttaac
     2221 tttcaaacaa tcctgttgaa taataactaa ttctattgta ttattttagt tgtagaataa
     2281 aactaaatcg gcaaaactag agaaatttag tgacgcaaag ctatagggac taagacttat
     2341 ataaatatct tatgagttat gtcagccagt tgccaaagag atagtgttct ttttgttttt
     2401 atgaaagttt tttaggggga taataattta tatgtttgga aataaaatgg ataaatgtac
     2461 acatgtattg actgcttata ttagcagttc gtatgattat tgtaattttt tagatacaca
     2521 gttagatgat tttatattag agtacggaga aagtgtagta gagtcttgtt tacaccaagt
     2581 gatggtattg gtaagtaagt ataattagat agacattgat ttctagagaa ccctttttag
     2641 ttttatctac atttatatta atatatagca atgtaaaacg tttaaatata tttttaatat
     2701 taagaataac taaaatgtta actaaaaaat ataattaagt agacatttct attaatctat
     2761 gatacaatag tatttgtggt aatacacaat taaatcaagt atatcttgat ataggttaat
     2821 aggagtattt ttaatgaata atggaatagt gaaatggttt aataatgaaa aaggattcgg
     2881 ttttatatca atggaaggta gagatgatgt gtttgctcat ttctcagcta tacaaacttc
     2941 aggatttaaa tcattagaag aaggtcaaca agtaagcttt gatatagtta aaggtgctag
     3001 aggtcctcaa gcagaaaata taactatatt ataatatata gatttttcgg gaaagactct
     3061 ttttagagtc tttttttatt gttaatatat gtaagataat cattggtgaa gtaaataagg
     3121 tatattgagt aatgttcttg atagtttata tgctaatgaa tttcaaaaaa ataagcactc
     3181 ctataaaaag agtgctctta atgtaaatat aaaccttcat tggtccagcc taataccccg
     3241 tattctccaa gttctaactc ttttttatat acattatcac tttcagccat atctaacaca
     3301 atcatttctt ctttagaatc aatttgtaaa atatgaggac tgtgtgttgt gacaattatt
     3361 tgtgttttta ggagaattgt ttttaatgta ataaaaattt tatataccac atagttgata
     3421 taaaccaaag tttggggtga tttgtttgat ttatttttat tcctttacat accacatagt
     3481 tgatataaac cgttttaatc ttatcaatac aaatttttct gaaagcactt tacataccac
     3541 atagttgata taaacctggt taaactagca gaggacatgg cagcactaaa tccctttaca
     3601 taccacatag ttgatataaa cccccaaaat aaacttagca tctccaatgt ttacacatat
     3661 acaatgctct taaaattgca gtgaacgcgc aatagtgcaa ttgataatac ttatcatata
     3721 ttctcaatac cttatatttc aactgctaaa cctcaattta tcacaaatat cgctcactgc
     3781 aaaatttcta tatttttatt atatcatagc atataattaa tattttcaat taatttttac
     3841 atttaattat gtttaaattc tataataatg caactttaat gtttctttct ggaactaata
     3901 taaaaactat ttcagaaaga ttaggtcatg cagatataaa aataactatg aatagctact
     3961 ctcagttttt agaagagatg gacagagaag catttgaaaa tttgagcaaa atactattta
     4021 ttatgtttta aagaagatga cttattcaat atttgatgct agaatataat taataaaaat
     4081 ttaattatag gtgattgtat tatgaatatt attaaaagca taactttact cttaacatta
     4141 ttaattgtga tattaatgac aggctgttcc ccaagagaaa gtgctagtga agttgcaaaa
     4201 aaatatattg ataagatgaa ctctggtgac tatgaaacag catatagttt attaagtaaa
     4261 gatagtaaaa aaaaattagt atagaaaatc ttgaagaatt tcaaaatatt ttagttagta
     4321 caaaaaaaat aattagttat aaaataggta aagaaaaaat cataaaaaaa tataaacatg
     4381 atggaaaaga atataaaaat gttgttaaat tagaagaaac ttttaatata aaaaaccttc
     4441 tttataaaaa agatgattct ctaaaagtaa atagatattt tgtagttgaa aataacaaat
     4501 acaagctatt attgtatgaa agtttcaaaa aagaatttgg tattaatagt atggaccttg
     4561 ctcaacttaa gcttaatgat tttggactaa aatactttaa agaagttgac cttatactaa
     4621 aaaaagctat tgctattaat cctactgatt caaagcttta ttatgcccaa gcatgtaatt
     4681 atatgaattt tggtggattt cctggttatt cagatacaac atataatgct ttagattcaa
     4741 ttaataaatc tttaaagtat ttagataaaa atgattctga ctacaaaaaa aatgcaagta
     4801 atatatataa cttaaaagga gttattctta tgtttcacaa aagatttgat gaagcacaac
     4861 aatatttaaa taaagcctta tactttaaca aagataatga agacccaaaa tataatctaa
     4921 atacaataaa aaaacatcta aactagatat ttaatttttg aatctataaa atattttttt
     4981 atatataatt cagtactgaa tcataatctc tactaaacta attacaagtt taaattataa
     5041 agacttattt ctatataaat tttactctat aacaataagt ttcttattta aattttaaaa
     5101 tattatctaa aaatcataaa aaaaaaggta taaagagcta ccctcaagct caatatacct
     5161 tataaatatc taaacttcat tttttactaa tttttctata tctttgataa tatgttcttt
     5221 agattttttt ccagctatgg catcaaaaat tgcaacagcc tttttctctc caaacttttc
     5281 tgctacatca ttaaagtcta aaaatctaac actttctttt ggtttaccaa acatataaca
     5341 cctctcatac atttttatat atttattata acttctatag acttattttt caccaataaa
     5401 cgttagacaa attagtccct ttattttttt aaactagctt taccttcaaa attcttaaca
     5461 aaaacagttt attctactct ataaataaaa atagacaatt taatttgtag gaaactgata
     5521 atagaataca gtaaataaaa ctttgtttaa tctatctgaa aaagttatct attaaaactg
     5581 ctatgaattt ataaagaata ttctagttca taaaaataca aatatgtaaa tataatcacc
     5641 ttaatattaa ttt
//
LOCUS       DAEMHW010000214         5567 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEMHW010000214 DAEMHW010000000
VERSION     DAEMHW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.214"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(203..886)
                     /locus_tag="KPK67_003318"
     CDS             complement(203..886)
                     /locus_tag="KPK67_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBG2460238.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(1184..1774)
                     /gene="zapA"
                     /locus_tag="KPK67_003319"
     CDS             complement(1184..1774)
                     /gene="zapA"
                     /locus_tag="KPK67_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBG2460239.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(1799..4192)
                     /locus_tag="KPK67_003320"
     CDS             complement(1799..4192)
                     /locus_tag="KPK67_003320"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBG2460240.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(4209..5228)
                     /gene="pheS"
                     /locus_tag="KPK67_003321"
     CDS             complement(4209..5228)
                     /gene="pheS"
                     /locus_tag="KPK67_003321"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBG2460241.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEKINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(5278..5512)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1695 a         1087 c          600 g         2185 t
ORIGIN      
        1 aaatacttct attaatctat atattgccta ctatacttaa gacattttaa ttaatttata
       61 cactgcttac tatacttaaa atacttctat taacttatac attgcttact atattttaaa
      121 ttctttaatt aatctataca atatttgcta tatataaagt atatcaaata acagcttcat
      181 ttttttgtaa atttacagaa agttaatttt cattaaatga atcatcttta ttgataaaca
      241 taattccaaa gaccactgcc aaaataaaaa aacatattag tattgatttt tccacgaaaa
      301 tttttgtaaa aaaagttcct aaaatagctc cagatataaa gaatataata atagcataat
      361 actgtaaact cttttttatc ataaaaatat tcttagtatg tatataatga aataaagttt
      421 ctgtagcact tcttaaattt ccagtacaca ttgtcgttgc aaaactattc ccatttactt
      481 ttctaaaact ctctacttgc attgaacaaa taaaagaaat agcaacattt acaaacatat
      541 taaatcttcc tctaggaata aatgaaacaa taagtattgt aatcatttca attataatga
      601 taacctgacg ccaatgaatc tttgtcttat tatttgtaaa atactttctt atcttctcgg
      661 ctataaatac acctaacata aatgccaaaa taggaatcaa ataataaaat gattccttta
      721 cattaccttc tgctatatta agcccaaata aaactatatt tccagtttgt gcattagcaa
      781 agactttacc tcttaccaca taagtataaa tatccaaaaa tcctcctgtt attgccaaca
      841 aagaacataa aaagaatgat tcagacatct gactcttgct tgtcatatat aaacctctcg
      901 ctttctaatg atagatacaa aatcaaaatc attttagatt ctatttttca ttgatataaa
      961 aaaattattt atatttaaat attgcctata tttatacatc atttatattt atacattatt
     1021 tgtatttata tatcattcat atttatttat atttaaacat tatctatatc tatatagcat
     1081 ttatatttat ttacatttaa acattatcta tatttatata gcatttatat ttatacatta
     1141 aaagaagctg tctcaaaaaa caagagacag caaatctttt agtttattcc acattttttg
     1201 catcattatt taaccctgta cattttaact gtaaatcata agctttgttt tgaaactcag
     1261 aagctaagtt ttcagctata gtagctcttt catttgcttc ttcaagttga gtacttaact
     1321 cctcaatctt gtttttataa gcatctaact ctgctctaga accttctgtc gtattactaa
     1381 gttcgaatat ttcttgcttt ttattttcta taatctcaat taactctttc atcttagttt
     1441 cagcttcacg attttctgct tctttttctg ctatcgtaag ctttaaagat ttgatttcta
     1501 attgaagctc ttcattagat gctccaacct tcttactcaa ttcttcattc gctttaatca
     1561 acttctcatt ctcatctgaa cattcaaata aaaggtctga tatatttaat gcagttaata
     1621 tcgcagcaac tgataagctc aatttcgggt tttgacgtgt tattttatcc atttctttgt
     1681 ctacaaaatc agcaatacta atcataaact tctctgattt gtctccaacc attggatatt
     1741 cagcgccatg gattttaacc attactttgt tcatcctaac ctcccccgac attagttgtt
     1801 aatttgatct taagttagca tttaattttt cgcttaattc acttaatatc ttatcatgca
     1861 ccttagctac atcttcatct gttagagttt tatctttact tctataagtt attgaatatg
     1921 caattgactt gtatccagct tctatttgag aacctttgta aacatcaaat aacttgtagc
     1981 tttctactaa tccttgacca ttttccttta ttatatcttc tatttgtttt acaaatacat
     2041 catctttaac aataagagct atatctcttg atgtagatgg atattttggt aatggcacat
     2101 aattctttgt tctatcagaa ttatcaaata caaagtcaat atctatttca gcgacataaa
     2161 ctctttgacc tagattgtag ttttctataa catctggatg caattcacct aatgtaccta
     2221 catatttatt attatacact attttagcac atctaccagg gtggaaggta gtattattat
     2281 cttgaggttc aatttcataa cctttaaatc cgacatttac aagtatagtt tctatagttc
     2341 cttttagtgc aaagaagtca acatctttac catacatacc tacacacatt ctattttctt
     2401 ccttaggtaa ttctgaatct tgtgggatga atatatgtcc acactcaaat gcagatactt
     2461 cttctatttt atgagataca ttagttgata aaacatctaa catatttggt attaaagtag
     2521 ttctcattac agaagtttct tctcctaatg ggttaagtaa tttaacaaaa tttctctttg
     2581 cattaccttc tggtactctt atcttatcta ctcccttagg gcttacaaat gaatatgtca
     2641 atatttcatt taatcctaca gcagttgaat tatcttttaa agcatccatg aatttttgtt
     2701 ttgaagtttt aattcctgct gtcgtatttc cttctaattg tacagaaggt atattctcaa
     2761 atccataaat tctagctatt tcttcccata catcagcttc ttgttccata tctgttctaa
     2821 agcttggtac atctatttct aatttatcat tagctaccaa attacattta aactctaatg
     2881 attctaaaat tcctacaaac tgctccattg gtacatctac acctaatagg tggttaattc
     2941 tttgaggatt tactaccaat tttttaggtt ctggtttatt tggatataca tctacaacac
     3001 cttttaaaac ttttcctgct cctaacattt caacaagttg tgcagctcta tttgctgcta
     3061 tctctgcaag attagggtct aagtcttttt catttcttga agatgcttct gacctaatac
     3121 caactttttt agctgtcatt cttatgtttt ctggtttaaa attggcactt tcaaaaagta
     3181 cagtcttcgt attagaagtt atttctgagt tagcaccacc cattacacca gctaaaccaa
     3241 gtgatttttc tccatttgtt ataactagca tatctttgtc taatgttctc tcaacatcat
     3301 ctaatgttac aagtttctct ccatctttag catttcttac tactattctt ccagtctcta
     3361 cttgatttat atcaaaagca tgaagtggtt gaccaagctc taacattacg aagtttgtta
     3421 tatcgactat gttacttata ggtcttactc ctgcttctgt aagtcttctt tgcatccaat
     3481 atggagaagg ttctattttt acatctgtaa ccattctagc aacatatctt ctacataaat
     3541 ctggattatc tatctcaact ttgaaatcta tctcttcgtc actttctttt acttcgattt
     3601 caggatattt tacttttgtt cctatagttg cagctgcttc tctagctata cccatcatgc
     3661 atttacaatc aggtctgttt gaagttaatt caaaatctat taaagcatct tttaatccta
     3721 aaacatcttt tatatctttt cctaattcat aagaatcttc gtggtctaaa atatatatac
     3781 cacctctttt atactcctca atgtaatgtt catctatacc tagttcagct gctgaacaca
     3841 tcataccatc tgataattca cctctgaaat cagtctgttt gatttcaact cctccaggta
     3901 acttagaacc atttacagct actggaatat aatctccttc tgatatattt gtagctcctg
     3961 taactatttg aacaacttta tctccaatat ctactttagt tacaaccaac ttatcagcat
     4021 ttggatgttg ttttatttct aaaatctttc caaccaatat attttctatt tcttcaccat
     4081 aataatctat tgtttcaact ttagttcctg tcattgtcat tttatcagcg aactctttta
     4141 catccatgtc tatatcaaca tagtctctaa gccattttaa agatactaac atctacatac
     4201 cctcctaatt aaaattgatt taagaatctc atatcatttt cgaataataa tctaatatca
     4261 tctatttcgt atttaagcat tgcaagtctt tcaaccccaa ctccaaatgc aaatccactg
     4321 taaacttctg ggtctattcc acaatttcta agcacatttg gatgaaccat acctgaacct
     4381 aatatttcta tccaaccttc atatttacac attgggcaac ctttaccacc acatttgaaa
     4441 caagtaacat caacctctgc acttggttct gtaaatggga agttgtgagg tctaaactta
     4501 gttttgatat cagaaccaaa caatttttca acgaagatat ccatagttcc tttaaattct
     4561 gccatagtaa catcttttcc aacaacaagc ccttctatct gatggaacat tggtgagtgt
     4621 gtagcatctg gcgagtctga cctaaaacat ctacctggtg caattacttt tattggtaac
     4681 tcttgacttc tcatagttct tacttgaact ggagatgttt gagttctaag taatacccca
     4741 tcattgatat agaatgtatc gctcatatct cttgatggat ggtctttagg agcgtttaat
     4801 gcgtcaaagt tgttttcaac agtctcaact tctggccctt ctgctataga gaatcccatt
     4861 ccgataaata tatctgttac ttcatctata atttgagtta ttggatgctt cttcccaacc
     4921 tttaaaactt ttcctggttg agtaacatct atcacttcct cttttaattt tctttctttt
     4981 tcaatagcat ttattttttc ttttttagaa tttatgctaa gttcaatgtt ttctcttacc
     5041 tcattggcaa ccttaccaac tactggtctt tcttcagcag ataatttacc catttcttta
     5101 agtatggcag ttatctcacc tttttttcct aagtacttaa ctcttaaact ttctacactt
     5161 tctatgcttt gtgcttcttt tatttcagcc aaagctgctt cacgtaaagc aagtaatttt
     5221 tcttgcacac atatcaccct ttcgtttttt tgtataaaaa aagcccctca atccctgtct
     5281 agggacgaaa ggctatagtt tcgcggtacc accctagtag tttaacaatt taaaaatcat
     5341 caaaccacct ctaacaaact taacgcgttt ttgacgtcaa agcctactgt aaaatttcag
     5401 ctctgttact ccagagggaa cttctcatga cctcttagaa aacactccca gcctaggtgt
     5461 ttatctcttt actaagttta atcaaaatac tttcctcttt cattgcattt acaatattat
     5521 attgtttatt ttagtattat atcatttcca ttcaaattat gtgacta
//
LOCUS       DAEMHW010000215         5553 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEMHW010000215 DAEMHW010000000
VERSION     DAEMHW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.215"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..693
                     /locus_tag="KPK67_003322"
     CDS             <1..693
                     /locus_tag="KPK67_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2460242.1"
                     /translation="IILLIVIGKIVQDDKLNLEYELINERIKTNYRNYENINEVHDRL
                     KYVYHDLKNHMICIKNYDTKEEIISYINNLEFQISEFEHFKNTGNKTLDLILAEKISI
                     CKKYDIQIEDNINISKLNFIKNNDICSIFANSLDNAIEACMDINNEIEKRIEVKATYI
                     NKFAIIKFINTKVNDIKLIDKRIQTSKDDDKIHGIGLASIKYIVNKYGGEIIVNYSDN
                     EFILKIMIPIKS"
     gene            705..845
                     /locus_tag="KPK67_003323"
     CDS             705..845
                     /locus_tag="KPK67_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG2460243.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            904..1485
                     /locus_tag="KPK67_003324"
     CDS             904..1485
                     /locus_tag="KPK67_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG2460244.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYRYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            1742..2116
                     /locus_tag="KPK67_003325"
     CDS             1742..2116
                     /locus_tag="KPK67_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460245.1"
                     /translation="MNLKKKIKKLGVTFAIGTIIVSSLGTSVFADEVSTYALISKAFS
                     GKNNIISSSVYTSGSRTFKTTDSQGGFSCRLTERFSGSSPDRIVKRHEANASSKVSLS
                     GGTYYIRYEGSTNNAKTYATLN"
     gene            2199..3908
                     /locus_tag="KPK67_003326"
     CDS             2199..3908
                     /locus_tag="KPK67_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460246.1"
                     /translation="MSSIKVISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTKMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERNYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKVNSENIKFNIKKDLIEVNLKESLDPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSYMAYKINLSSKSNVYSNLPLLNKTSNFFKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKSSV
                     DAKKYVDEVDNVMNSIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            3923..4795
                     /locus_tag="KPK67_003327"
     CDS             3923..4795
                     /locus_tag="KPK67_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2460247.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLIDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            4796..>5553
                     /locus_tag="KPK67_003328"
     CDS             4796..>5553
                     /locus_tag="KPK67_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460248.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVLISIIIVFYIA"
BASE COUNT         2303 a          497 c          823 g         1930 t
ORIGIN      
        1 ataatacttt taatagtaat agggaaaatt gtacaagacg ataaattaaa tctagagtat
       61 gaacttataa atgaaagaat aaaaacaaac tacaggaatt atgaaaatat aaacgaagtt
      121 catgatagat taaaatatgt ttatcatgac ttgaaaaacc atatgatttg tataaaaaat
      181 tatgacacta aagaagaaat tatatcatat ataaataact tagagtttca gataagtgaa
      241 tttgaacatt ttaaaaatac tggaaataaa acattagatt taattttagc agaaaaaata
      301 agtatttgta aaaagtatga tattcaaatt gaagataaca taaatatatc taaattaaac
      361 tttattaaaa ataatgatat atgttcaata tttgcaaatt cactagataa tgcaattgaa
      421 gcatgtatgg atataaataa tgagatagaa aaaagaatag aagttaaagc tacatatata
      481 aataaatttg caataataaa atttataaat actaaggtaa atgatattaa acttattgat
      541 aaacgtattc aaactagtaa ggatgatgat aagatacatg gaataggact tgctagtatt
      601 aaatatatag taaataaata tggtggtgaa ataatagtta attactcaga taatgagttt
      661 atattaaaaa taatgatacc aataaaaagt taggagagta caaaatgaaa aaaatagcgt
      721 taaacttact taaaaacatc tcagcattaa gttttgggat agctgtatta tcagcaaaca
      781 gtgcaagttc ttgggtagca catcaggcaa aagagccaca agctttacaa aaactaaaaa
      841 aataacatta ataaaaggac ctcttcttat taaaggagag gttttacatg ttgaggagga
      901 aatatgttta aaagattatc atataaattt gccaatatat tagttaataa tgagattgta
      961 gaaagtgaag attttgaaat atatagatat ggatttgaaa ctttgattta tttcatagtt
     1021 aatattttgg tagcattatt tataggaatt atttttgata gatttataca tacagttata
     1081 tttttaagtt gttattgcac tttaagacag ttcacaggag gttatcatgc taggaattat
     1141 aaagagtgta cattaacatt tgcagttatt tatttgataa ctattttttc agctaataat
     1201 atagatataa ataaatatag gtatttatta gtattattaa tgattattag tatactaaca
     1261 atttacaagt tagctccttt agaacataga aacaaacctc ttagtgaaag tgagaaaaag
     1321 cattatagaa agactgtgca aaaaatacta ttcgtaataa tatgtttaat tattttatgt
     1381 aaaatactta atatttttca gcaatatgtg atttatgcaa taatttctat atattggatt
     1441 gctatattaa tatatattgg aatgaaggtt aatagtgacc agtaagatta taaaacattt
     1501 tctaaattaa ttaacttcac taattaatat taactttaaa aaaataatgt caaggtaagt
     1561 atttgaactt attaaatata gttgaatcta ttgtgggttg tatcaaagga caaatagatt
     1621 cacttaataa ccagtttgta acagagtatt gaattgtaaa agttaatacg atagtatgaa
     1681 aatgtaattt gaaattacat ttttataaaa attatattaa ctaaggggag ggatacatac
     1741 aatgaatttg aagaaaaaaa ttaaaaagtt aggagttaca tttgcaatag gaactataat
     1801 tgtatcaagt ttagggacat ctgtatttgc agatgaggtt agcacatatg ctttaatatc
     1861 taaagctttt tcaggtaaaa acaatattat atctagttca gtatacactt ctggaagtag
     1921 aacctttaaa acgacagatt ctcaaggggg attttcttgt aggctaacag aaagattctc
     1981 aggctctagc ccagatagaa ttgtaaaaag acatgaagca aatgcttctt caaaagttag
     2041 tttatcagga ggaacatact atataaggta tgaaggttca acgaataatg ctaaaacata
     2101 tgcaacttta aattagtatt aaaagcatcc tataattatt tttataggat gtttttgtaa
     2161 ttattataat caaaagactg gctaggaggg gcataaatat gagtagtatt aaggttatat
     2221 cagactttat aaccaaaata attagaaaaa atattttatt tgcaataaca attattatta
     2281 taaattacca tctcatacaa cgatggacac atatatccaa tagaggaatt tcatttaatg
     2341 attacttatc cttgagtgta tatacttgta tgacaattag tattttaacc atgttaaatg
     2401 taatttttta tatgagaaaa agttatgata ctgtaaaaat attatgtaga aataattttg
     2461 attatttttt aggtatttct gttgctgtaa ctagaaacaa ctttatattt tgttgcatac
     2521 caattatata tgctatagca tactcaagtt taataagaac attgtcaatt gtaacgatta
     2581 aatcaactat atttatattg atatttgaat ggtttttacc acttactatt ttatctatag
     2641 tgatatcatt tatttcagtt attataaata atttaatact aaagattacc ttatcatcag
     2701 tatttttata tttaacatca actaagatgt tggatagagt tattgatatt aaagaaccat
     2761 tttttaaagc attatttaaa tcacttaata tatttgaaga tcaatcatat gcttactatt
     2821 ctgattttgt agggaaagtt aataattcag catatgttat agataagatt atacctacac
     2881 tatttgcaat tactttaata atagtagcat atctgatatt aaatgatatt agaaagaaac
     2941 agattaaatc attaatagtt atagtagtgt acagtatttt atttattgga ttaaattata
     3001 tatcaataga tatgtcaaca tatagagatg aggagcgtaa ttatggagag tatttaaaga
     3061 aatctaatat tagtgatatt aaaataaaat cgcatgatat gaagattgac tttaaaagca
     3121 agtcaaatat aaaagataga attgttttat taaacacatc aaatgaagag ttaacaaata
     3181 ttaatatgat tttagatgaa atatttgata taaaatatat aaaagtaaat tcggaaaata
     3241 taaaatttaa cataaaaaag gatttaattg aagttaattt aaaggaaagt ttagatccta
     3301 atgacgaggt gacattagat atagagtatg aaggctatat taatgtgttg acatcttgga
     3361 atcaaaataa atatatagct actaaagatg atattatgct tccaccaggt tctttagcgt
     3421 ggtacccaaa agtaaatcaa tcaagttata tggcttataa aataaattta agttctaaaa
     3481 gtaatgtcta ttcaaattta cctttactga ataagactag taattttttt aaaaaagatt
     3541 atatatttga aggaaagtca aatgatattg caatatatgc aggtgagttt aaagaagagg
     3601 taaaagatga tataaaaata atctatccat cagatagtaa tgtaaatatg gatatagagg
     3661 aaaaaattaa atttttaatt gaaacaagaa atgaaatatt aggtgatagc aaatcatctg
     3721 tagatgctaa aaaatatgta gatgaagtag ataatgttat gaattcaata aaaagaaaaa
     3781 aaattaatat gatattagtt gctgacttac ctagtaatga atttttatat gaagactcaa
     3841 agggagagaa aattagcata actatttatc attggctttc aggaaatatc cttataatag
     3901 ataaataata aaggaggtta atatgataaa caagttagaa ataagaaatc taaattttaa
     3961 ttacaaaaat aaaaaagctt tggataatat aaatttaacc ctaatagatg gagtagttgc
     4021 attattaggt cctaatggtg ctggaaaatc aacattaatg aggttattag tgactcttta
     4081 tgaaacttca attggagaga tagaattaaa taatataaag tatagcaaaa acaatgagaa
     4141 aatcaaggca aatgtaggat atgtacctca agattttgat atgtataata atataaatgg
     4201 gcaagaatac ttagaatttg tagctaaaat gagaggtgta tctaagaatg atttaaagaa
     4261 acatatacaa aaagtagtat caaaagttaa tctggacaag tttataaata aaaaaatagg
     4321 tacatactca ggtggtgtaa agagacgact aggtatagca caggctttaa taggtgattc
     4381 taagttaata gttatggacg aacctacagt tggtcttgat ccagagcaaa ggaatgaatt
     4441 tagaagattg ttaccagaaa taagtaaaaa ttctatagta ttaatttcaa ctcacatagt
     4501 agaagacata caatttaact gtgacaaatt aattatatta aatcaaggca aaatattata
     4561 tgatggtaca attaataagt ttattgatat ggttgaggtg tattcggtag tagtttctaa
     4621 tgatgaattt gaatatttag aacgtaatat tgatattatt gattatagga gagttaaaga
     4681 tggtgtgcaa gtaaagtata taggtaaaaa tacagagaaa cttaaagata gcttaaggat
     4741 agatacaact ttacaagatg cttatataaa ctttttacat aaggagttaa aataaatgtg
     4801 tatattggag ttaataaaat ttgagcttaa aaaaatagag cttaaacatg tgttaatatt
     4861 tgtaattatt tctatcttat gcatatttat tacaggcata ttagattata atgagttaat
     4921 attttataat gatgtaccta taatggatac atattctata gcaggatggt taggaattat
     4981 aaagatatca gatgttttta taccaataat gtcagcaatc acaataattt atgtgttttc
     5041 aaaagattat tcgggtaata taaacgagat acttacttta tataataaaa aaaaatataa
     5101 tcaatttata gtatttagat ggtgtgttat attagctata tatactgtat tattattaat
     5161 ttcaattaca atagtatata catttacgtc agtgaaaggt tttaacatag acaaaataga
     5221 ttttgtttat agtgtaaagc caatagatgt gtttttaaag acatttccta cattattatg
     5281 gtatacaaca tttccactaa tgatattaac attaacaaaa aatagattta caacaatagc
     5341 aatattaatt acatatacat ttgctgatat attttttatt ttacatatgt acccatttgt
     5401 tagtatgatt aatgtaaatg gattttatat tcaaaagatg ttttatatgc ctaacaatat
     5461 agatgttaga tttaatgaat taaagaatta tttctttata aatagaggtt ctctagtact
     5521 tatttctata ataatagttt tttatatagc aag
//
LOCUS       DAEMHW010000216         5544 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEMHW010000216 DAEMHW010000000
VERSION     DAEMHW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.216"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            287..1123
                     /locus_tag="KPK67_003329"
     CDS             287..1123
                     /locus_tag="KPK67_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG2460249.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1476..3053
                     /locus_tag="KPK67_003330"
     CDS             1476..3053
                     /locus_tag="KPK67_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG2460250.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            3046..4230
                     /locus_tag="KPK67_003331"
     CDS             3046..4230
                     /locus_tag="KPK67_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2460251.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     IL"
     gene            4248..4847
                     /locus_tag="KPK67_003332"
     CDS             4248..4847
                     /locus_tag="KPK67_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG2460252.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFIHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4871..5341
                     /locus_tag="KPK67_003333"
     CDS             4871..5341
                     /locus_tag="KPK67_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG2460253.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         2098 a          575 c         1045 g         1826 t
ORIGIN      
        1 tgaaatactt gaaaatactg gaaaaattga aaataatttt tgtaatataa aatgtcgatt
       61 gattttaaat aattaaatat acataagaaa taattaaata tatttcaatg gaaatatgat
      121 aaaatatgac aaattaattg atgacaataa aaaatatttt gttattgtag tattataggg
      181 taaattgcaa aatatatacg aaagtaaaat tttaaatatt gacattaaat taaatatact
      241 atactatgta agtgtgatga gagaattttt ttagagggga tttatagtga atataaaaaa
      301 gatatttaat aacaatgtag tagtaagctc cctagaagat ggcacagaaa taattgtcac
      361 tggtgctgga gtaggattta agaaaaaagt aggcgatttg atagatgaaa atttgatttc
      421 taagaaatat tttgttcaag acgatcaaag ggacaaatac aatcaaatac ttaataagac
      481 ctctattgaa tactttaaaa tttcagaaga aattatagaa aaggctaatg aagttttaaa
      541 tactcaagtg aatgattcaa taatattagc cttaactagt cacatagaat ttgctgtcca
      601 aagagaaaaa caaggtataa aattaccaaa cttaatattg aatgagacaa aacaacttta
      661 tagggaagaa tttgaatttg gattatgggc aattgatgaa attgaaaaaa ggataggaat
      721 aaaattgcct gaagatgaag caggatatat tgctattcac ataatcaatg gtttggaaaa
      781 ttctcaaaaa gatgaaggta ttaatatact agagttttca aaacaagtta ttcagataat
      841 tgaagaagta catggattta aattggatgt aaattcatta aattatacta gacttataac
      901 tcacttaaag ttttttgttc aaaggattct ccgaaaagaa acttacaatg atagtgatat
      961 tgaagatatg tataagttag tgaataaaaa ttacaataaa cctaaagtgt gtactgaaga
     1021 aattgcagca ttggtattag acagatttga atataagatt agtaaagaag aagaattata
     1081 tttaatgatt cacataaata aaataacaaa cagtggttgt taggattgta actgataaag
     1141 caggcaaaac ctaagtcatt gagcttaact attagattaa gttttttgat ttaggttttt
     1201 ttattagata caatcaaaat acagacagtg acttttagga ttgtaactgg ttaagcaggc
     1261 aaaacctaaa tcattgaatt taactattag gttaagtttt ttgatttagg tttttttatt
     1321 agatacaatc aaatacagac agtgactttt aggattgtaa ctggttgagc aggcaaaacc
     1381 taaatcatta aacttaatta ttaaattaag ttttttgatt taggtttttc tataaatttg
     1441 attatgtttt tagataaaaa taggagggga aaattatgaa aaaaaataag ttaggtttat
     1501 ggaacttctt tcaaatgtta ggaaaaacgt ttatgttccc aatagcacta cttagtgttt
     1561 ctgggatgat gctaggactt ggggcaggtt ttacagaccc taagatgatt gaaatggttc
     1621 catttttagg gaatgaatat gttaacttag ttttaaactt catgttaaca ataggattat
     1681 ttgcatttaa caacttggga gcattatttg caatggcaat tccactagga ttattaaaaa
     1741 aagaaaaaga gtttggtgct ttttcaggat tagtaggatt tatagcaatg cacataggta
     1801 caaattttta tttaacaaga gctgatttgt tagtaccagc agaacagatg tcaactaatg
     1861 gtcaagctat gattatgggt atacaaacat ataatacaag tgttttaggt ggcattgtag
     1921 caggattaat tgtttatgca atatatgata aagttagtaa tttaaagata ccagaatcat
     1981 taggattcta cagtggacca agattagttc caattgttag tttaatagtt atgagtatag
     2041 ttggattatt aatacctatt gtttggccac catttttcaa tgcattccaa agtttaggaa
     2101 gatggatttc ttcagcagga ccattaggat atttttcata tgcagtggca gaaagagtta
     2161 caattccatt tggactaaat catcttgtaa catccgtatt ccgttttaca ccaataggtg
     2221 gaactgctgt tattgatgga gaaacgtact ttggtacatt gaacatgttt atggcatatg
     2281 tagaaaataa tcagattata cctcttgatt tagctggtaa aatggaacaa ggtaaattga
     2341 tgattcaata tggattagct ggtgctgcat tggctatcta ccgtacagca aaaccagaaa
     2401 atagaaaggc acttaaagga cttttaatat caggagtgtt gacagttatt ataggtggta
     2461 taagtgaacc aatagagttt ctattcttat tcatatcacc agcattattt gctttccata
     2521 catttatgaa tggtctagca aacatggtac ttccatactt aggcgtgttg atgggattca
     2581 caggagattt aattgcattt ataagttttg gtgtgttaag aggaacagct acaggatggc
     2641 caatagcagt attagtagca gttttatatt ttgcaatata ctattttgta tttaagtggg
     2701 caattattaa gtttaatata aagaccccag gacgtgagga taaagaaata aaagtatctg
     2761 aaggaaatgg ttcagatttc aaaaatttgt ctacatataa aggacaacaa atgatatctg
     2821 ctttaggtgg tcaatctaac atattatcac tagataattg tgtaactaga ttgcgtttaa
     2881 aattacaaga tgtatcatta ataaatgagg atgcaattaa agaggctggt ggtatagcag
     2941 ttgttaaatt agatgagcat acaatacaag taattatagg aactcaagtg tattctttaa
     3001 gaaagcagat ggataaagct atggaaggtc atttggaggc atgtaatgat taattttgat
     3061 aaaaaagtag accgattggg tacatattgt acccagtggg actatgtaga agatagattt
     3121 ggtaagaagg atttgcttcc ttttacaatt tcagatatgg atttagagtt tccatttgaa
     3181 ataacagagg tgttgcagga aaggttaaat catagagttt tgggatacag tagatggaaa
     3241 catgatgatt ttagaaattc agttacgaat tggtatatta aaagatttaa ttgtagcgtt
     3301 gatagtaact ggatatatta tagtccaagt gtaatgtatt caattagcaa acttattgag
     3361 atgttaagta atgaaggaga tggaatcctt ataaatactc ctgcatacaa tgctttttat
     3421 gatgtgataa caaataacaa tcgtaatatt ataaagtcac atcttgtaaa taggaatgga
     3481 ttctattcaa ttgattttaa tgattttgag aaaaagtgta aaaaatctaa gatatttatt
     3541 ttatgtaatc ctcacaatcc aactggtaga gtatggagtg agttagaact tgttaagatg
     3601 ataggaattt gtaagaataa tgatgtcaag ataatatcag atgatataca catggatatt
     3661 gtatatgaca atagaatgac acctatctta aatgttgcaa aggactattt agattctgta
     3721 ttcatttgta catcagcgtc taaatctttc aatataccag cattgactgg gtcatatgtg
     3781 attataccaa atgaaggtat tagaaataaa tttgaaaata tcactagata ccgtgatttt
     3841 gtaaattcgc cagcaattct agcaatatta gctacaatgg tctgttatga tgactgcgaa
     3901 tattggttag atgaactatt aaaatacctt aaagagaatc ttaaatatac gattgattat
     3961 ataaaagata atttagaacc attgaaattg gtcatgccag aaggctgtta ctttgcttgg
     4021 atagattttt ccgaacttaa aataagtggt gagaaattcc aaaaattatt gatggatatt
     4081 ggagaagttg ctattatgtc ggggtctgta tatggtgaag aaggtgagtt ttatttaagg
     4141 ttaaatgtgg catgttcaag agaaaaactt gaagatggtt taaataggat taaaaaaact
     4201 atggattata ttgcaaaaag aattttatag taggaaggga gatagttatg ggatatttaa
     4261 aaacaatggt tgatgttatg actactgaaa tgaatgccat aagatgttta atagatgaag
     4321 caggaataga atatgaaagt atagttagtg aaattgcaaa ttgtgaaggc aaagtaattt
     4381 ttatgggtgt aggaaaatct gcacatatag gtaagaaatt ggcagctaca tttgctagta
     4441 ctggtacacc tagctttttt atacatgcaa cagaggcagt gcatggagat ttaggaatga
     4501 ttgaaagtaa ggatattaca attttaatat caaatagtgg aaattctatg gaagtggtaa
     4561 actgtataaa atatattaaa gcaataggtt ctaagacaat tgcatttact tcaaacagaa
     4621 attcagtact ggcaaaagag tgtgattatg ctttaatata tcctgcaaag gatgaagcag
     4681 accatttaaa tctagcacca acaacatcat ctacaattac tttggtttta ggagattcta
     4741 ttgcatgtgc attatctaag agtagtaatt ttggttcttc agatttttat aagtaccatc
     4801 caggaggaag tttaggtgag aagttaaaaa cagctaacaa tggataaaag caaaaaggag
     4861 agtagttttt atgttcaaaa tgtttaaaaa aaaatcaata aaatcaccca tatcaggaaa
     4921 agtaatagag ttatctaaaa tacaagatgg tgtattttct caaaaaatta tgggagaggg
     4981 tgtagctata gattctacag gagacattgt atatgctcca atcaatggaa agatagtagt
     5041 agtagcagag actaaacatg catttgtaat agaattagaa aatgggatgg aacttcttat
     5101 tcatgttggt ttagatactg taaatttaaa aggcgaaggt tttgaggcat tggtgagtat
     5161 gggagaacaa gtgaaggaag gaacaccaat gttgaaaatt aaccgtcctt tgattgaatc
     5221 aaagggtata tcacttatta ctccagttac aataactaat cattcagagt acaatatgaa
     5281 tacatgtaat gtaggcaata gtgttgaagg tgggaaagat actgtaattg agtttaaata
     5341 aagaactaaa atacaaggtt aatatttata aatacctata aacttatgtt ttataagatt
     5401 gattgtatag atttgttgta taaaaaagaa ctctttaatt ataaattaag gagttctttt
     5461 ttattttgta aatattgttt agatttatat agaaaaacac ttagtaataa atagaataaa
     5521 taagtattta tatagctaaa aatc
//
LOCUS       DAEMHW010000217         5518 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEMHW010000217 DAEMHW010000000
VERSION     DAEMHW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.217"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..547
                     /locus_tag="KPK67_003334"
     CDS             <1..547
                     /locus_tag="KPK67_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBG2460254.1"
                     /translation="ISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLLQ
                     IEALASTKNLDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSLSQSKLYISVSV
                     RNKYLKFVVTDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGI
                     LYIENSVKTGGAMVTIEIPIV"
     gene            complement(623..1444)
                     /locus_tag="KPK67_003335"
     CDS             complement(623..1444)
                     /locus_tag="KPK67_003335"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBG2460255.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            1680..2849
                     /locus_tag="KPK67_003336"
     CDS             1680..2849
                     /locus_tag="KPK67_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBG2460256.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKETYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            3032..3232
                     /locus_tag="KPK67_003337"
     CDS             3032..3232
                     /locus_tag="KPK67_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG2460257.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            3653..4081
                     /locus_tag="KPK67_003338"
     CDS             3653..4081
                     /locus_tag="KPK67_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG2460258.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(4224..4979)
                     /locus_tag="KPK67_003339"
     CDS             complement(4224..4979)
                     /locus_tag="KPK67_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2460259.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
BASE COUNT         2163 a          660 c          826 g         1869 t
ORIGIN      
        1 tatctcaaag ggtgtcactc aaatagaaaa atatattaat actttaatag atatatcaaa
       61 aacagagaat ggctatatat taaataaaga agcaatcagt atgagtgagt ttataaaaga
      121 tgtcttactg caaatagaag cattagctag tactaaaaac ttagatgtgg agttttataa
      181 acaagataat ttgcctaaaa atataactat agatagagaa ttgttattca gagctattat
      241 gaatataatt tcaaatgcta tagattattc actaagccag agtaaactat acataagtgt
      301 atcagtacgt aataaatact taaaatttgt agttacagat tgtggaagtg gtttttctaa
      361 agcagatttg tcaaaagcta cagaacagtt ttatacagga gatttgagta gaaattctaa
      421 atctcattat ggaatgggac tctatattgt aaataatatt gtacaaaaac ataatgggat
      481 tttatatatt gaaaattctg tgaaaactgg tggtgctatg gtaacaattg aaataccaat
      541 agtttagtaa ataatatcca ttttgtaaat acctaaattt tatgatttat aggtatttat
      601 aaaatggata gtttttttga ctttatatat tagttaaatc tgataaaaag ttttcaaaca
      661 taactccatc atttctatca gtatcaaatt gagatgtgta atttatagtt ttatatataa
      721 aatctgttgc agtattaacc gctactccca aatcatgttt atttactaac ataccacaaa
      781 gtatagatgt gaatatatct ccagttccac tgtaagaaca attattgtat ttgactgatg
      841 taaagaatac atggtcatta tctctatcgt aagccaaatt taatatatta tcatcttcta
      901 atataccagt aatcacgact ttgctaggtc ccaaatcaga tacagatttt gcaatatata
      961 taagctcatc tctattataa tcgcttttgg tataatcatt tccagttaag aaacaagctt
     1021 ctgttaaatt aggagtagtt aaatctgaat gtttaactaa ttctttaatt tcctgtctca
     1081 tttcttctgt gaatattgga tacataactc cattatcacc catcacaggg tcaactacga
     1141 taaaagcatt gggataatcg tttataaaat tagccactat ttcaatttga tgctttgaac
     1201 ccaaaaaacc actatatata ctatcaaaat tgacttttaa atttttccaa acatttgaat
     1261 attttaccat ttcatcagta aagtctaaaa aagtaaattc aggataacca gtttgacttg
     1321 aaagtatggc cgttggaaat ggacaacatt gaacctttaa tgctgaaagt ataggtatag
     1381 caaccgataa tgagcatttt cctattccag ataagtcatt aattgctgcg actttttttt
     1441 gcataaaaaa ccctccatta gtatgtatta attataatga taccaaaata tttatataca
     1501 tatatattac tataaaaaaa agaaaaaagt agattataaa ataaaaaatt gtaaaaaaaa
     1561 cagtgtattg atacaattaa tttttaatat tggacataat aaaatagaga attattaaat
     1621 atgaaaatta ctatatgtga cagaatattc tttgtaattt gatttgtaag gtgtactatt
     1681 tgggaggtgt agctttgaaa atactgaaaa ataagaataa gtacatcaaa ggtataatgg
     1741 catttgtatt tttgtcttta gctactacag caggtgtatt tttaatcaat gatttaagta
     1801 tgaaaaatat ggaagaaaaa gtcatggaat ctgcaaatgt tgaaggtgat gatgtaaaag
     1861 accaatataa actattgctt gaaaacttat ttgactacag aaataaggca atattagaga
     1921 aaaatgaaaa cattttaaaa ggtctttatg agacggataa aaaatttggt ctgtgggctt
     1981 atgaaaatga agtaaaaaag atgaagtatt tagaaaactg gtctagtaag caaggtgtaa
     2041 aatttaaaga tataaagaca agggttaaga ttaaaaaaat aaaagaaaaa gaaaaagacc
     2101 tctatggtat aatctgttca gtatcgactg aatataagta ttcatatgaa aatcaaaagg
     2161 acaaagaaaa tgcctttaga ataggtacat atcattatct aaatgtaaaa attatagata
     2221 atcaatatgt cataactaaa gagtggtata cagacccttt tgcagactct ttaaatttag
     2281 aaaatataaa atctgatgat ataagaaatt atattgggca acaagatggt gtagagttac
     2341 agcttacaga tgaacaaaaa aaggctatag attatgcaca taaatactgt ggagccgcag
     2401 ctgatgaaga acatggtatg aaatttaatc caaactatag ggattataat ccagaaggtg
     2461 gagattgtgc aaactttgca tcacagataa tgtttgaaag tggaaggttc aaaaagaact
     2521 caatttggaa ttatagcgag agagcaggta caaaagcatg ggtaaatgca caagctttta
     2581 aaaattatgc actgtatagt ggaagaggtt cattaattgc taaaggttcc tatgaagaag
     2641 tatacaaaga aacttacaat ttacgtccag gggattttgt tggttatgag aagaaaggca
     2701 gaataacaca tgtttcgaca gtgacaggat tagactcaag aggatatcct ttggtaactt
     2761 gtcataatac tgatagaatg ttggtgccat gggatttagg ctggagtgat aagtccataa
     2821 gattccatat aataaaaata aattattaaa aaattataaa gaaaaataaa gtgtaaaaga
     2881 taataatcat ttaaaattat aaagtaaaaa aattgattat taatgtataa aaataataat
     2941 aattgacaaa catagctata tatgatagag tagtaataag gttacagaaa agagtagcta
     3001 aatatttgtg tttttttagg aggatttatt aatgaaaact ggtgtagtta aatggtttaa
     3061 caacgaaaaa ggatttggat tcatatctgt agaaggtgaa ggagatgttt tcgtacattt
     3121 ctcagctata actggagatg gatacaaaac attagaagaa ggacaaagcg ttgagttcga
     3181 agtagtagat ggagctaaag gtcctcaagc tgctaacgta gttagattat aattaaaaat
     3241 tcatatatat tagtgttcta gtaatatgag agataagccg ctaatcattt agcggttttt
     3301 tcagtttttg taaataaaat taatagcaaa ataaattaaa attattaaaa taaaatcatt
     3361 aataatgaga taaatatgtt aaaattaata gtgtagagta aaattattat ggctaagttt
     3421 aagtcaatta atttttagta aattttctaa aaagagaata agtgatactg atagaccata
     3481 taaaaatatt aataaagcat aataagatta aaatgagcaa aggaaaaacg cttgttacag
     3541 cgtatagtat taagaatact tagaaaataa gaaaggagaa agttactgca aaaaagtaac
     3601 attataaata ttgagtaata tttataagtt ttcagtaacg gtgggtagtg taatgaacat
     3661 attattcttt ctaacaccaa aaagtgaagt agcatatata tatgaagact acactataag
     3721 gcaagcccta gaaaaaatgg aatatcacaa atactctgca attccaataa taagtaagga
     3781 tggtaaatat gttggaacta taacagaagg tgatttttta tggacattaa aaaatgattt
     3841 aaatctggac ttaaagggat tggaagatgt tccagtgaca gatataaata gaaaaatgga
     3901 taattctcct gtttccatca acgcagatat tgaagattta gttataaaat ctttaaatca
     3961 aaatttcatt cctgttatag atgaccaaga cacatttatt gggataataa aaagaagaga
     4021 tgtaataggt tactgttatg agataatacg tggatataaa aatttagcta atgataatta
     4081 aaaatcttat ttaaactttt aagctgagtt ctttacaata aaagaatgat aatatgaaac
     4141 tgaatattta aactttaata taaaaagctg tcttttctaa taaatattgt gaaaagacag
     4201 cttttttata tatgacctta tgattaatcg ttgtatttta caaggtttcc gtacttagca
     4261 ttaagagcat ttatcttatt tccataaact tcattttgtt tttgatataa taaacttttt
     4321 tcaacttcag ccttaacttc atcaaatgta gactctgtac tttcttgaag gtcttctaat
     4381 tttattatat ggtatccaaa ttgagtttta actggttcac ttacttcacc tttagccata
     4441 gagaacacag cttcttcaaa ttcaggaacc atttgtcctc taccgaatgt tcctaaatca
     4501 ccacccatat ctttagatgg gcaagaagaa tgtttaagag ctgcatcttc aaaagatatt
     4561 tcttctgatt ttatttgagc aagtatttct ttagcttttt catcactatc tactaatata
     4621 tgtttagcag ttgcactttc tggtttagag aaagagctct tattagcttc aaagaatttt
     4681 actttttcat cttctgttac attaacacta gataaaactt tatttatagc aaattgcttt
     4741 aatacatttt cttctatctt tttcatttct aatttaaata tttcttcttc atctaatttt
     4801 tcttctttag catctaagaa gaataattct tgattcacta aatcatttaa taagtgtttt
     4861 tttccttctt cagttttaaa ttgcatagct tgataagggt ctaatgattt taatgcattt
     4921 tcaatatcaa tattagttat ctctttttct ccaactgttg ctaaaacttt tttttccata
     4981 atagttaact ccttcatagt gtatttcatt attaatataa gactaccatc ttaaataata
     5041 tcagaaaata atgtagatgt ctaatattta aagccatatt aagtcaaata gaattaaatt
     5101 tagtagaaca aagttattac aattaataaa agtgagctca taatacccac tacaccttcg
     5161 tcatctttaa atttaagagt ttttactatt gaataataaa ggtatattcc agtaaaaata
     5221 atctttaaat acaagaaaaa taaactcttt gggtatatta aaaagctaaa aagtgtaaat
     5281 ataaaagcaa gtagagtact tatcctgtaa aagaaatcta tttgtgtaat gtctttatat
     5341 tttatcataa acaccgaata taaaatgatg aaaacaattg cgacgattgc atatatggta
     5401 aataacatat tttcaagagt accatttgaa attactatcc acttatacca cataattggc
     5461 caaaataata gtgctaagaa cttaaaataa ttatcaaaca acttttcttt atacattc
//
LOCUS       DAEMHW010000218         5473 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEMHW010000218 DAEMHW010000000
VERSION     DAEMHW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.218"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(625..2031)
                     /locus_tag="KPK67_003340"
     CDS             complement(625..2031)
                     /locus_tag="KPK67_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2460260.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAVFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(2076..2783)
                     /locus_tag="KPK67_003341"
     CDS             complement(2076..2783)
                     /locus_tag="KPK67_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2460261.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKEGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2954..3868
                     /locus_tag="KPK67_003342"
     CDS             2954..3868
                     /locus_tag="KPK67_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460262.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQNGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3868..4629
                     /locus_tag="KPK67_003343"
     CDS             3868..4629
                     /locus_tag="KPK67_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460263.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMISITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRSHLLKKEV"
     gene            4641..5378
                     /locus_tag="KPK67_003344"
     CDS             4641..5378
                     /locus_tag="KPK67_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460264.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1901 a          841 c          699 g         2032 t
ORIGIN      
        1 tttatttata tattacaaag tttaaatttt ttcattaatt atattataaa tttcccatat
       61 atttcaacat aaatacatat atatatatgt tatttctaaa aatactacac aaaaactcta
      121 ctttagtaga actcaatggc gcaaaatact tcaatttaaa acatttatcg tactttaaat
      181 aaataactat aataaaatat acttacaata gttacataga agctattata actacacaga
      241 acagcttata attaaaactt ttttaggatg tcattatatt ttttcttaaa attaaaaaac
      301 actataccat aattctattt tagtagaatt tttttgtcaa taataaaatt aaatattaac
      361 taaaaaataa aaaaaatgga attatctaaa gaatttaata accttacaat aattccaaaa
      421 tattatttct atgaaaattt tgataccatg agtaactcag ccataagcgc ataaagcaca
      481 agttactctt tactttaata aaacttgaca agaaaagaat ttatccttat tctactctaa
      541 tagagttaat ttgtcaataa agaataaaat tattttcaaa atattttcat tcttcattct
      601 tatctgttat tattttataa aatatcactt aattagaggt aattttagta ttgtttttag
      661 tccctgacta tcaatgctcg taatatctaa tttacctcta tgtgcattaa taatgccaga
      721 tactatcaaa atacctagac catgattttg acctttggca tatgcctcac ttagtccatt
      781 ttgactcttt aataaactat taattctttc ttgactcata ccttttccat catcttcaac
      841 aattatatag caaaaatcta aatcagactt agaggtaacc cttatattac atccttgttg
      901 gttatgagtt atactatttt gtattaaatt actaatagcc ctttttaata attttttatc
      961 tgcatctata ataatatctt catttagtat atcaagttca acgtaaaagt cattatccaa
     1021 attattattt ataaactcag aaataagctc tctcaaaaac gcagaaactc taatcttatc
     1081 acaattcaat gcttgcatat tatattcaag tttagacact aaattgaggt catttactaa
     1141 ttctctgatt ttaatccctt gattgcatat aatagacact tgctcttgtt ctctatttga
     1201 aaggctactg ctttcttcaa gttctccagc atatccaatt atcattgaaa gtggagttct
     1261 tatatcatga gatattcctg caatccagtt agacctagct tcctctttct tcctaagttt
     1321 ttcatctttc atttgcaact ctttagatac agtattaata gattttgaaa tatcacttag
     1381 tattcctttt tcttgtaaaa gtactggttc tccttttgac ataagcttta tgccagtaat
     1441 aattggcttt atagatttca ccatttttaa acctatgaat agagctatta aaaaagtaac
     1501 tattgcatta accagaaaca tacctattat taacatagga aattgttcta tttccttaac
     1561 cgtataagta aggttaaact tagtgtactt gtcttttgga taacctgtta ctaatattcc
     1621 atttggatgt tcccatacat acacaggata gtcctttaaa aagaaccttg aaaactttgc
     1681 tacatctgtc aaagtataat gacttggaat ttcttttggt aaatcaaatc cccatattct
     1741 atctccattg ttatcaatta acattgccca aactttattt ttatgtagta cttccttact
     1801 agaatcatct aaaataaact ttccatctct taaaatcaaa ttatcttcaa tcataatcaa
     1861 gtttgatttt atttctctgc tatcacttgt atctaaatta tcagagtatt tagatacaaa
     1921 tagcatatat gaaaacagaa gaaaaactgc tatatttaat acaataaaaa gtaatgaaat
     1981 caagatactt gaacctataa atcgcctcaa tatatacttt acactatcca aaattaaatc
     2041 actcctcttg atttctttta ttctaatatc taaaattaat taatcatcaa cttatatcca
     2101 agacctctta ctgtaataag atgtttaggt gtagatggag ttttttctat cttctctcta
     2161 acccttctaa catgaaccat aagggtattt tcatatccat acatatcatc tccccaaatg
     2221 gcttgacata gtgaatcatt agtaacaatt ctccctttat tttcatacaa ctttaaaagt
     2281 aatgaatgct ctttagctgt taaagaaatc tcttgccctt ctttctcaac acatgcacta
     2341 tttaaattca caacaacaga atctcccagt ttaaatactg gcaactcctc ttcttctata
     2401 ggcacatata cacgatttaa tatacccaca agccttaaag taagttcctt tggtaaaaat
     2461 ggctttacta tataatcgtc agcccctaag cccaaaccaa tcaatctatc ttcatcttca
     2521 cctcttgcag agagaaaaat cactggtatt ttagatgtct ctctaatttt tctcataata
     2581 gaaaaaccat ctccatctgg caatacaaca tctaaaattg cacaatctgg ctttacattt
     2641 ctaaataccc taacagcttc ctcataactt gatgctaaaa atactctact aaatccctct
     2701 tttctcagga tttcatctat catttttaat aattccttgt ggtcatcaac cacaagtatt
     2761 tttttatctt ttattgactc cataatcatc accctccttt agtataccat atacccattt
     2821 tcacaacttt gcctaaatag acttttaagg ttattgtaag gtaaacttca gctaactgaa
     2881 atgtaaccaa gttattatac tgatataagt taaaagaatc aataagcaca gattaaaaaa
     2941 ggggatgaaa catatggaaa tgataattag aacacaaaga ctttcaaagg aatacaataa
     3001 aatatttaga gtaaaggata tagacctaag agttccacaa ggagctgtat atggtttttt
     3061 gggtcctaat ggagctggaa aatctaccac tcttaaaatg cttcttggac ttgctaaacc
     3121 cacacaagga aatattaata ttttaggtaa agagttaaat gaaaaaaata gaatatcaat
     3181 tttaaaagat ataggttctt tgatagaatc tccatcatac tatggtcatc ttacagggct
     3241 tgaaaacatg accataatgc aaagactctt agatttacct aaaaaaaatg taaatgaagc
     3301 attaaaaatt gtaaggttag aaaatcaaaa aaataaaaaa gttagtcaat attctttagg
     3361 tatgaagcaa cgtcttggca ttgctatggc aataatgaag tttcccaaat tattaatact
     3421 tgatgaacct acaaatggac tcgacccagc agggattgag gaaattagag aattaataaa
     3481 atctctacca gaaaaatacg ggatgactgt tctaatctct agccatcttt taagtgaaat
     3541 tgaccaaatt gccacatcaa taggaataat aaatcatggc gaactggttt ttcaagatag
     3601 cataaaagaa cttcataata gaggtcaaag tcagattgct atcaaaacta agaatgttgt
     3661 atctgcacaa aaactattaa atactaaagg atatatgtca cttatacaaa atgggtactt
     3721 gactattgaa aatttaaaag actcagatgt tgctaaaata aatggtatgc ttgtccattc
     3781 aaatatagat gtatttagaa tagaagaaca taaaaaaagc ttggaagata tattcttaga
     3841 acttactggt aaggcggtga ctttataatg aaataccttg gtctagaatt ttataaactt
     3901 aaaagaaaga agattattct gatgaccttt ttatttgtag ctgttgaaat aatgtggtgt
     3961 atcgtaaatt caaatagagc tattacacgt aatccagata tgttaggtgg atttgaatat
     4021 tcctatatgc ttatgagttt tgcaagttta aatggtctat tctttcctat cttaatatca
     4081 ataattacat cacgtataag cgatatagaa cataaaggag atacatggaa gcttttaaaa
     4141 tcttctgtga catctttaaa tagtatttat ttatctaaat ttttatgttc tgctattttg
     4201 gtatctatac ctgtattgat gcaagtgtta tctattgtgt tgtttggaca ttttagaggg
     4261 gttattgagt caatttctgt atctttactt ttaaaataca cattaggaac aattcttgta
     4321 agtattgcta tcattgcact acacctatgg attgcaactg taattccaaa tcaaatgatt
     4381 tcaattacat ttggcatgat tggtagtttt ataggtttga catcaggttt gttttatcaa
     4441 ggtattcgta gattgtttat atggtcttat tacctagagt tatcaccttt aagttatgct
     4501 tatgataatg ttttaggttc atctgtctat agaattgata tgaatttttc tattacgatt
     4561 ttagttttct taattggaat gctaatttat tatattggaa gaagtcattt attaaaaaaa
     4621 gaagtatagg agggatttaa atgttaagga agtcagtctt gggagaaatt ctaaagttaa
     4681 aaaattcttt tatatggtat gtattattag ctttgccact tataagtgtg ttgattggtt
     4741 ctggtaattt ttatctaaat caaggcatac tcaaaaaaga atggtacagc ttatggactc
     4801 aagttagtct tttttatggg gaatttttct ttcctatctt aatcgcaatc ttttgtgcat
     4861 atatttgtag gcttgaacat atgaatcata attggaataa tgtaatgaca ctgccagtta
     4921 atttaaaaaa tatcttttta tctaaactta cagtagtttc aatacttaca gctattacac
     4981 aagttttctt tgttatcttt tatatagttg caggaaatat gtttggtttt tctggtagtg
     5041 tacctaaaga attgactggt tggattgcta aaggttggat tatttctatt tcaatagctt
     5101 ctatacaatt atacatgtct ataaagatta gaagttttgc aactcctatt ggtattagtc
     5161 tttgcctttc tctatttggt atgggattaa tagtagcaaa acctgctgtt ggtatatttt
     5221 tcccttattc tatgttaggt agtgctatgg gggttataaa gcaatctgct ttaaatgtta
     5281 atgacactat agttacaata tgtgttgcat ttattactac tatagtattt tctgaaattg
     5341 ccaatagaaa ttttagaaag aatgatgtta tttcttaatg gtaaatgcta gatattttgg
     5401 ggtttgaaat ttatattctt tttagttgaa atctatatcc attttattta aaaatatata
     5461 tcttttattt gaa
//
LOCUS       DAEMHW010000219         5468 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEMHW010000219 DAEMHW010000000
VERSION     DAEMHW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.219"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            193..396
                     /locus_tag="KPK67_003345"
     CDS             193..396
                     /locus_tag="KPK67_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2460265.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            560..1714
                     /locus_tag="KPK67_003346"
     CDS             560..1714
                     /locus_tag="KPK67_003346"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBG2460266.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVEVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1727..2731
                     /gene="rseP"
                     /locus_tag="KPK67_003347"
     CDS             1727..2731
                     /gene="rseP"
                     /locus_tag="KPK67_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBG2460267.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2914..3969
                     /gene="ispG"
                     /locus_tag="KPK67_003348"
                     /gene_synonym="gcpE"
     CDS             2914..3969
                     /gene="ispG"
                     /locus_tag="KPK67_003348"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBG2460268.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            4317..5141
                     /locus_tag="KPK67_003349"
     CDS             4317..5141
                     /locus_tag="KPK67_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460269.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKRSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
BASE COUNT         2130 a          538 c         1031 g         1769 t
ORIGIN      
        1 gttttagtaa aataatgaga taaatatata taaagtttaa tttttgaact tatgcttttg
       61 ttttatttta acatatttat atttaataaa ctataattga aatctattta gataaataat
      121 tatttatcta aatagatgaa aattttccaa aatagataaa atgtgataga atatattttg
      181 cgaggtgata acatgatttc atacgctcca ttatggaaat tgttaattga cagaaaaata
      241 aagaaaatgg aatttgtaaa tatatcaggt ataagtattt ctgttttagg cagactagga
      301 aatgataaat ctgtttctat ggatacaatg gagaagatat gtttagcact agactgtaaa
      361 atagaagatg ttgtggaaat aaaaaagggc atttaataat actagaatat tttataatat
      421 tccacaagat acaaaaaata atagattaat tttaatctgt ggaataatat atgaaatttc
      481 tatgaatagt aaaaataatt gttttagtag tgttataatg ttttgacagg gtatatttat
      541 tattagaaag gattattata tgaaaaagat atcaatttta ggttctacag gctctatagg
      601 taaacaaact ttggatgtgg ttagagaaca tagagataag tttgagatag ttgcaatatc
      661 agctaacagt aatatcgaat tattgctaga acagatagta gagtttaaac ctaaatatgt
      721 aacagttttt gaggaaaata aagcattgaa attaaaagaa atgttgccta aaaatataga
      781 aatagaagtt ttagcaggaa tggaaggatt aaaaataata tcatcacttg atgaagtgga
      841 agtcctttta actgctgtag taggtatgat aggattagtc ccaactcttt gtgctataaa
      901 gaagggaata gacatagcat tagcgaataa agaaacgttg gtaactgctg gtgaacttgt
      961 aatgaaagaa gcagaaaaat acaatgtaaa tattcttcct gttgatagtg aacatagtgc
     1021 tatatttcaa tgtctaaatg gagaaaataa aaaaaatata gaaaagataa tacttacagc
     1081 atctggtggt ccatttagag gaaagaaaaa gggagaactt gcaaatataa ctaaaaacga
     1141 agctttgaag catccaaatt ggagtatggg aagaaaaata agtattgatt cttcaacgct
     1201 tatgaataaa ggacttgaag tgatagaagc tagatggtta tttggagtag aacaagaaaa
     1261 tatagatgta gtagttcacc cacaaagcat aattcattca atggtgcagt atacagatag
     1321 ctcaataatt gctcaattag gatgtccaga tatgagacta ccaatacagt atgctcttac
     1381 atatccagat agaatggaaa gtagctttga aagaatgaat ttttcaaaat ttagtacttt
     1441 aacttttgaa gaaccagatt tagaaacttt tccatgttta aaattggcat atgagtgttt
     1501 aaaaatggga ggaacttatt cttctgtttt aaattcagca aatgaagttt tagtaagtga
     1561 atttttagag gataaaattg gtttctatga cataccatat tacatagaga agactttaga
     1621 agttcatagt agcataagtg aaccaacatt agaagaaatc ttagaaacag atagatggag
     1681 tagagcttat gttgcaaatc tgataaaaaa ataggaaggt gaatttatga ctataatagc
     1741 cgcattaata ctatttagta taattgtatt aatacatgag ttaggacatt ttatatttgc
     1801 aaaaagaagt ggtatcaaag taaatgagtt ttctataggt atggggccta agatatatag
     1861 tgtaaaaaaa gatacagagt attctataag agcacttcca attggtggat atgtcagcat
     1921 ggaaggtgaa gatgaagaac aaataagtcc aaattctttt ggaaacaagt ctatattaca
     1981 aagatttagt actattgtag cagggccaat attcaatata atattagcag caatactttt
     2041 agttccagta tttttgtata taggttctcc aacgactaaa ttaggaaaga taatgccaga
     2101 tactcctgca caggctgtag ggcttcaagt aggagataag ataaataaaa ttaatggtaa
     2161 ttctgttaag acttgggatg aagttgcaaa tataataaat acttcttctg gtggagaatt
     2221 aaaacttagt ataactagag atggaagtga taaagttgta aatgtaacac ctaaaaataa
     2281 caatggaaag tatgaaatag ggattcaacc acaaagagaa aaagactttt tggggtcaat
     2341 agtaaatgca tgtaaaacta ctgtagatat gacaaaacag atgctaacat ttctaggtca
     2401 aatgattaca ggacgtgtac caggagggat tggcaatgct gtagcaggac cagtaggtgt
     2461 tattggtatg gtatcagatg ctgctcgtac aggcatcata aatgtagtgt atttagcagc
     2521 tgtcataagc ttaaatttag gtatagtaaa tctattacca ataccagcac ttgatggatg
     2581 gagaatactt atgctgttac tagaagctgt tagaggtggt aaaaaactcg acccaaataa
     2641 agaaggcatg ataaatgttg ttggttttgg agctttaatg ttatttatgc tttttatcac
     2701 atataaagat atactaagat tatttcaatg atttagtttt ttagagattt taagaaaata
     2761 ggctatgtag ttttaaataa gaataaatta atttttatgt atgaaattta ttcttattta
     2821 actagatagc tttttaattt ataatataca tgtaggttac aatattttaa tatttaaaga
     2881 gattcaatat aaaataatat atagaggtgg aatatgagtt acgagagaag aaagacgaga
     2941 gaagtaagtg taggaactgt aaaaatagga ggagaaaatc ctataagtat acaatctatg
     3001 acaaatactg atacaagaga tgcagatgct acaatagctc aaataaagag attagaggaa
     3061 gctggttgtg atatagttag agttgctgtt cctgatatta aagctgctaa aaatatagca
     3121 aagataaagt ctagtgtaaa tataccaatt atagcagata tacatttcga ttataagcta
     3181 gcccttgaag ctatagaaca aggtgtggat ggaataagaa taaatcctgg taatatcgga
     3241 agtatagaga gagttaagat ggtagttgag aaatgcaagg aaagaaattt aaaaattaga
     3301 attggagtta atggaggttc tttagagaaa gaacttttaa aaaagtatgg ctctgcaact
     3361 gctgaagcat tagttgaaag tgcaatggga catattaaga ttcttgaaga cttagatttt
     3421 cataatatag ttatatctct aaaatcatcc gatatttata aaactgttga tgcatatgaa
     3481 ttaatatcaa aaaaagttga ttatcctctt catattggaa ttacagaatc tggaagtgtt
     3541 cacaaaggaa caataaagtc ttctataggg gtaggagcac ttcttcttaa aggaattgga
     3601 gatactgtta gaatatcttt aacaggcgac cctgttgaag aggttattgt tggtaaacag
     3661 attttaagaa gtttgggact tttaaatgat aagattaaag ttatatcatg ccctacatgt
     3721 ggtagatgta atatagactt aattagtgtt gtaaatgagg ttgaagaaaa gattggtagt
     3781 atggaaaaaa atatcactgt tgcaattatg ggctgtgctg taaatggacc tggtgaagcc
     3841 agagaggcag atataggtat tgctgggggt aaaggtgaag gtcttttatt taaaaagggt
     3901 gaaatagtta gaatgattga tggtaacaaa ttagttgatg agttgctaga agagattgaa
     3961 aaattatagt atacaattca tatatatatt aattgactag atttttaaat acttttgtaa
     4021 taagttttta tttacagtat ttaattatct agtcattttt tattttataa acttcattaa
     4081 atgtaagaaa cttatattct aatttgacta tatatgtatg agttaaatca tattgtaaat
     4141 ttataaaatt aaaaaaagat ttaatatagt gaaaatttcc atcaatagta ttgaaacttg
     4201 atagtaacta tgataggata tagttactag aacagttggt aaattatgtt aacatggatt
     4261 ttaatgtttt ggtaaattgt tttattataa gttaatagaa aaagaggtga agaactgtga
     4321 gatataaaaa aatatctagc ttacctattt tagttgttgc tttgctgatt actggaacga
     4381 caacaacatt ttctcttagt acgaccaatt cagaaaaaac aaatgtatac ttagcaaata
     4441 cggtaagtac tgtcacaaag aaagtatcag atattcaaag tagtaaaggt cttgtttata
     4501 atgagaatat tccattattt ataaataata gcaaaataca gtatgatgat actccatatc
     4561 attggcctag caatgttata agcttaacaa atagcagtga aaaggcaatt atggattatg
     4621 aaattacatg cttagcatat gataaaaatg gaaaaccact tgagctatat tgggatgctc
     4681 aaaatgtggc agcagatggc gaagtaggaa gtgttggatt tagcccagct ggtgtagact
     4741 atggtattgt tacaggcata tctcctgtgt ctccaaaatc atattcacat acttatagaa
     4801 aaatgcaaca atcaccacca caggatataa taagtatgtt tgaaaaacaa caaggcaagg
     4861 catgggtaga aaattggttg aaagaatgga aacaaatgga gaaagaatat gcaaagcaaa
     4921 atgcaatagc accaggtaag aatcaaaatg atgcctttct attgtttgat aaatggaaac
     4981 gatcaactgg agagcatggt gtaaagtaca ttatatcttg tgtaaaacaa gtaacattta
     5041 atgatggtag tgtatggaaa aactcagcat atgagaattg gttgaaaagt tttcaaggga
     5101 aggaagtttc aaatagtgta ttagaaaatt actataagta atgtttaatg atagcaaaat
     5161 gagtacgaat ttaattaatg ttaatttaat taaataagga ctattttgtt taaaaatatc
     5221 agtgttacaa ggaaattttt attgtgacaa aatgatatat atacataaga ttataagcat
     5281 gtgtcaagtt ttgatacatg cttttttatt ttgaatatat gttttctata aatttatgat
     5341 tttttagtta tgtaatttta tatgtgaaat tttagatttt tatatagata gtgaaataat
     5401 gtggaagttt tatgttttaa aaattttagt taaaacattt attaaaaaag ataatagaaa
     5461 tgttttaa
//
LOCUS       DAEMHW010000220         5468 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEMHW010000220 DAEMHW010000000
VERSION     DAEMHW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.220"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            171..668
                     /locus_tag="KPK67_003350"
     CDS             171..668
                     /locus_tag="KPK67_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG2460270.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(844..1092)
                     /locus_tag="KPK67_003351"
     CDS             complement(844..1092)
                     /locus_tag="KPK67_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460271.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(1158..2174)
                     /locus_tag="KPK67_003352"
     CDS             complement(1158..2174)
                     /locus_tag="KPK67_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG2460272.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(2232..2999)
                     /locus_tag="KPK67_003353"
     CDS             complement(2232..2999)
                     /locus_tag="KPK67_003353"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBG2460273.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(3067..5256)
                     /locus_tag="KPK67_003354"
     CDS             complement(3067..5256)
                     /locus_tag="KPK67_003354"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBG2460274.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         1698 a         1034 c          532 g         2204 t
ORIGIN      
        1 tcttatattt ttctctttag tttcacacat caccaagtct aaactttata ttatatatta
       61 atatttttga cttgtcaagt tatttatgtt ataatattaa tcattagata attaaagcat
      121 tgtacctaag ttgcaatatc taaaattcaa tttggaggta taactagcat atgtataaaa
      181 ttggtgaaag tgtaatgtat ccaaaagagg gagcctgctc tgtaaatgat attgtaacaa
      241 agaaaataaa tcatgagatg caaaaatact atgaattaag tgtcattttt aatagtaacc
      301 ttaaaatttc gattccagtt ctaaatgcag atagaattgg catacgtcca gttatggatg
      361 gaaatgatgt tgataatttt atccaatcta ttaataaaac agatggtgta tggatttttg
      421 atagaaaaga aagacttaaa ctgtaccaag ataaatttca ttcaggagat gtttttgaaa
      481 ttgtaaaatt aatcaaaatg cttatgatac aagacagttc aaaacaattg tgtagcacag
      541 ataaggagtt tttaaataaa gctcaaaaat ttgctttatc agaattagct gctgctcaat
      601 gtaaatccta tacaatggta ttagaggaaa tgaaaaaaca tatattaaat tcaaagaata
      661 caaattaaac cagtttttta aactggtttt attttttata ctaatcatcc atgctacttt
      721 agcttatttt aaactgccta atctaatatt aataaagtta tctcaattag atagatgtat
      781 gttattcaaa taaaaaaaac taagagtaaa tcatattact gatttactct tagctaataa
      841 aatttaatct tcttcaagtt cttttttctc ttgtaataat acacttaatt catttgcttc
      901 taaaacttgt gcctctgcaa tatcaagtaa ctcttgttgc cattccacac atttatcttt
      961 gtaatcaaaa ggtagcgttc tgtcaactat gtacttatca atacagtcct ccgcactttt
     1021 aattatttct tttctttcat caataaattt ttctaattct actaaggctt cttcataagc
     1081 ttttctactc atataaattc cccctttgta caattattct acattaaaat gatttttcct
     1141 cttttttgta taaaatttta tttgtatata acttttccat caatatcttt tatagtttga
     1201 catccagtta atatcataga actacttagc tctgattgaa gtttatttac ataagtttct
     1261 acaccatcag ttgcaccacc aaatgaagca gtcacaaaag gtcttcctat taatactgca
     1321 tctgcaccta aacctatcat ttttagtacg tctaaaccag ttctcactcc accatctgca
     1381 agtattgtaa ctttaccttt aacagcatca gcaatttttg gtaaaacttc acatgctcct
     1441 ggtgtacagt caagcactct tcctccatga tttgatacga ctattgcatc cacacctgct
     1501 tctactgcca ttaaagcatc ttcaacagtc attatacctt tcaatataaa tggtagttta
     1561 gtagacttta ctaactcctt tatttgttta acatttttag gaagtactgg ttttccatgt
     1621 aatgataaag ttactagacc acaggcatct atatcaactc caactgcaaa tgcaccagct
     1681 tcttcagcta atcttatctt ttttataata ttttcattat cccaaggttt aataaataca
     1741 attccagctc cattatactt tttgactaca tctaaatttt ccattaaaaa agcatctact
     1801 gccgtatcac caaccattgc ataaatacca ctattagcac atcctgcaac tactggttct
     1861 atgtaatctc tttcattaat ttttccaccc atatttaaag tagttcctgt tactggagct
     1921 gcaaatatag gtgcactcat tttctttcca aacatctcta tagatgtatc aggatttgat
     1981 acatcatgta ttactctcat atttactttt actttttcta aactcttgct attttctata
     2041 aaagaagaac cacttccttt acctcccatg ccaggcactt cacctgcaca agcaacccca
     2101 ttacaaacct tacaaacttt acaacttcca tttaaatttt ctctagcact ttttaataat
     2161 tcattatatt ccatctttaa tcccccatta actatagtaa tttatattat atacattata
     2221 tcatattata tttacagtat atttttcaaa aaactttttc tgtggatagg agttatgcca
     2281 tatttttcaa tagcttcata gtgttcttta gttccatatc ctttgtgaga tttaaaccca
     2341 tattctgggt atactctatc atattggtac attatactat ccctagtaac tttagcaagt
     2401 atacttgctg ctgctattga tatagatttt gaatctcctt tcacaattgg attttgagat
     2461 atgtcaatac ctggtattgt tgctgcatca actaataaat aatccggtgc tttttttaaa
     2521 caatttatag cctttttcat tgccatatat gtagcattta atatattaaa ttcatctatt
     2581 tcttcattgt ttactatacc tattccataa tctagtgctt cttcttttat tacttcaaaa
     2641 agttcatctc tttttgcctc actcagtttc ttagagtcat ttacaccttc tatttttgta
     2701 tcttttttaa atactacaac agaagctaca acaggtccag caagtggtcc tcttcccgcc
     2761 tcatcaattc ctccaatata taaataacct ttatcatagc cttcgttctc aaatatattt
     2821 atagtttcca atctttcttc ttcttttcta atattatcca gcttttttgc tagttttata
     2881 gctagacctt gtacagattt tctttcatca actcttaaca gctctatata ttccatatat
     2941 ttttctactt ctaaagtctc tattatttct ttaatctccc tcacactttt atcctgcata
     3001 ccattccctc atctttcgtt tttaaaaatt taatttttaa atatattaaa attttttaat
     3061 tagagtttaa ttgcatccac tatagccaca ttttaaacat atactacatc cacctgtatg
     3121 tgcaattcct tcacttccac actcagggca cacaaatttt tcatcttctt caaaattttt
     3181 atttattatt tctttggctt tttcatcttt attatctttt tcttttttat tatttttata
     3241 atattctttt ttactatttt cttttttatc ttctttatta ttttcttcct ttttttcatt
     3301 tattaaaatt ccactccttt tacatccatc tctaaatatt gttactcctt taagaccagc
     3361 ctcccatgcc ttaatatata tattgaaaac atcttcaact gtagtttcaa aaggtatatt
     3421 tattgtagat gatatagaag catcaatata cttttgccat actttttgca tgtttattct
     3481 ttcttcatat cctatattca tagcagtaac aaaaaaatct ggtaaacatt cttcatctgt
     3541 aatacctttc ttgtccatat actctttaac aattggagta tatactttat aatacacatc
     3601 ctcatcatgc aagctttcag tttttcttat atatgaaaga ttaaatatag gttctattcc
     3661 tccactgata cctatcattg tagatataga acctgtagga ggtatggtta aaagttgtga
     3721 atttctaagc ccatactttt taaccatttc cttagtgtct tcatttagat tttctataaa
     3781 aaacttagat ttagaaatat tttcatattt atactcacta aatgttccat attctttagc
     3841 aattaatgct gattgcttca cagcttcatt taacatagtc ttaccaatta aatcacatag
     3901 ttctaaagat tcatcagaac catatcttat tcccattttt ataagcatat cagctatacc
     3961 cattacacct aaacctatct gtctatactt atgtacacta tctctttgtt cttgtaatgg
     4021 atgcaagttc aatccctcat ctaagacatc atttaaagct actacagctt ctctaacgca
     4081 agattttaat ttattaatat caaaagtagc atgttcacca aatggttgta ttataaactc
     4141 tgacaagttt attgagccaa gtagacaact tcctccactt ggaagtggtt cttctgcaca
     4201 aggatttact cctgaaaagc aaaagttctt atcttcactc ataaaattat atgtttttat
     4261 tttatcccaa aacaatatac ctggttctgc ataatcccag ttatttttca caaatttatt
     4321 aaataaatca taagcattta ctatcttaac tattttttct ccagtagttt caacttcaaa
     4381 agtacaatta tataactttt tttctcttac agcttgcata aattcatctg ttactcttac
     4441 agaaatattt gctttagtaa ccttctccaa atctgttttt atatctataa agtcttctat
     4501 ctctggatga tttacatcca tagaaatcat aagagctcct cttctccctc tttgaccaat
     4561 taagccagtt gtcatactgt aaagttccat aaaagagact gctccagtcg tatgttttgc
     4621 agcattgtta actttcgctc cttttggtct tagctttgaa atatcaattc caactcctcc
     4681 accataacta aaagttctag caagtttttt tgcagtatca aatatatctt ctatgctatc
     4741 ttttggtggt tctattacat aacaattaga atatgttact ttttttccct ctttctgtaa
     4801 tcctctattt gctaaaattc ttccagcaaa tagaaatctt ttttgtctta ttagttttga
     4861 tattcttgaa ctattatttg aaattctctt tatccattca tcaaaactct caccattatg
     4921 tctatacttg ttcttccata ttgatttttg taattcatca aactcccaag ctttagtcct
     4981 tatttcagag cgtctttctc tatataatat gtagtgtttt gctatatctt ttctatttga
     5041 ttccataagt agtacttcaa ctatatcttg tatttcttct acggataagc atttttccat
     5101 agaattatag tcattagcta tttttcttga tatatcatat gccaactctc tatctactcc
     5161 accttcacta tttatactag ctttatagac agcatcttca atcttacttt tactaaaaag
     5221 ttcttttgta ccatctcttt ttattatgta tttcattttt aaacctccaa taaattagta
     5281 ttaaattaat acacacatta actataactc taaacaaact ttttatatat ttgtatattt
     5341 tcatattaat tatctaacat aatatatatt atactatttt atattcaata taacactcct
     5401 tatagttttc ttaacttgta aaatatcttc ctaaaaaact caaaaataat ataaaaaagt
     5461 atataaaa
//
LOCUS       DAEMHW010000221         5405 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEMHW010000221 DAEMHW010000000
VERSION     DAEMHW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.221"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            223..2028
                     /locus_tag="KPK67_003355"
     CDS             223..2028
                     /locus_tag="KPK67_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2460275.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLSKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            2105..2644
                     /locus_tag="KPK67_003356"
     CDS             2105..2644
                     /locus_tag="KPK67_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HBG2460276.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            3268..4482
                     /gene="dpaL"
                     /locus_tag="KPK67_003357"
     CDS             3268..4482
                     /gene="dpaL"
                     /locus_tag="KPK67_003357"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG2460277.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLRDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            4815..>5405
                     /locus_tag="KPK67_003358"
     CDS             4815..>5405
                     /locus_tag="KPK67_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG2460278.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         2221 a          541 c         1039 g         1604 t
ORIGIN      
        1 tatgaggaaa gctttttttt attattgtat tgtgatttat tttattgtct gcatattata
       61 attaaggtta ctatataaaa caaacttttt ataaattaat attgtctgtt ttaacgcaaa
      121 tatataaata agtaatgttt tatatatatt ttaaagagtg actatatgtt aaaatatagc
      181 taaactgata atttggtaaa ttaaattcta atgggggaaa ctatgttaag taaattaaaa
      241 gaatttcaac aagaaatgat aaaatacact gaaacagtag ctagtgtgtt agatgtagat
      301 attgaaatag ttgacgatag attaataaga attagtggca caggtctgta taaatcaaag
      361 ataaatgaaa gcgttgttac agaaggattt atttatgaca atgttataca gacaggtcag
      421 gaattagtcg tattggatat atgtgataat cagctttgta tagaatgttc ccattacatg
      481 aaatgcctaa ataaagtaat aatagcagtt ccaataaaat ataataatcg aactatagga
      541 gtaattggtg ctattagtac agataaaact aaaaaagtag aaataagtgc aaaaatagat
      601 aattatttaa aatttgtcaa tcatatatgc gatttaattt ctatgaaaat agaagaacat
      661 gaagcgtcaa aaaatagcag tagaaaaatg gatatgatga tagaaatcat tgaaaatgtt
      721 gaaaaaggcg ttataatact agacataaat agcaaaatat cttatataaa caatatagct
      781 ctaaaaaaat tagatataga taaaaatata atagaaaata tagtaaatat agtatctgtt
      841 gaatcttcct caaatggaca tgaattacta gaaattgata ttgataataa aatttataat
      901 ataaatgcca aaataattcc ggtatatcca tatataaatc aatacgacaa aataataata
      961 tttgacaaga catatatcaa tcataaagga catggaaaag tgaatagtgg ctggggaaat
     1021 agtgatatag agtctataat tggaaattca gaagcaatgt taaaagtaaa agagagaact
     1081 aagaaattag caaagtcaaa ttcaactgta cttataactg gggaaagtgg tacgggtaaa
     1141 gagctaattg ctagagctat acatgctgaa ggtagcagat ggaacaagcc atttattgcg
     1201 ataaactgtg ctgctattcc agaaaatctc cttgaaagtg agctatttgg ctatataaaa
     1261 ggtgcattta gtggagctag tagtggaggt aaagtaggta aatttgaact tgcaaatgaa
     1321 ggtgttatat ttcttgatga aataggagat ttgtcaatgc ctttacaagc aaagttactt
     1381 agagttttac aagagagaaa atttgcgaga attgggtcaa ataaacttat agatttagat
     1441 ataagagtga ttgctgctac aaataaaaac ttattgaaat tagtaaatga aggtaagttt
     1501 agggatgatt tatactatag attaaatgta attcctataa atttgccacc tcttagggaa
     1561 agaaaggatg atattgaggc aataatgatg aagtttgctt ctaaatattc tttagaatta
     1621 ggtattcagt taagtaaaat agaagaaaat gtaatgaata tgttgattaa ctataattgg
     1681 ccgggaaata taagggaact ggaaaatgca gttgaatata tgatgaattt ggttggagat
     1741 gatggaataa tatataaaga tatgcttcca ttagatattt taaactatta taatattaat
     1801 ggaaatattt ataaaaataa ggatgtaaat ataatatttg aagatgatat agttggaggg
     1861 atagtagaga atcaagaaag gatactttca attaaagaat tagagttgac ttacataaac
     1921 aagttgttga ataagtatgg aagagataca aagacgaaga agaagatagc aaaagactta
     1981 ggcataggtt tggctacact atatagaaaa ttggaagagg aacagtagta atataatatt
     2041 aaaatgatag gtatttgtgt gtttttatag ataggtattt ttactttatg gtggaggtgt
     2101 tattatgcaa tatgcgatag agctatatta tgataaaaaa acggaaaagc aactttttga
     2161 tttatctaaa aaaatcgctg atgaaaaaat tagtacgaag tacttagaat ggaaaacaag
     2221 acctcatctt actttggctt gtttcaatga tgtggatgag gcttgttgca tagacaagct
     2281 aaaggggttt gcacaaaacc ataaggtaat acctgcttac attggttcag taggcatgtt
     2341 taatgatact aaaacggtgt ttgtatcacc tataatgaat agcagtatgt atcagtttca
     2401 gagagaatta tatgaggtac tacaagattt tgattcaaat ggttgggaat ggtaccaccc
     2461 caatcgttgg gtaccccatt gcacaatagc acttactgga gatgatgaaa gtgaggcttt
     2521 ttttcgggca agtgatgtat tactgcgtga gttcaaaaaa ataagtggtg agtttgtctc
     2581 aataggattg gttaaaatta catttcctgt ggaagaaatt tttacagtga atatgaatag
     2641 gtaactttaa ctctgttgtg gatgttatga atattttgta tagtgcattt catcagataa
     2701 atgcgaagtt gcgaatttcg acagctcaat agtcgcaagt agggcacaat ggtacacata
     2761 ttgcactttt gcttattggg agggacgctc atgtaaactt ctgtaagaag ctatgaaaag
     2821 atttaagcca ttttggaggc tttgggtaat aaagaatatt tgggtaataa agaatataaa
     2881 gtatataaat ttatagagtt tatgcgtaag aaggttatag ccatatattt atagaaaata
     2941 aaagtagtaa tatatctgac aaaacaatag acatataaaa aataagagca taataaagga
     3001 agaattaaaa taaataaacg atttctcgaa ttgataattc tcgtattgat aaaaatttcc
     3061 caatatgaga attttttctt ttataaatct tatttttcac atttaatatt tataaaattc
     3121 aaagaaattt tataaatata tgtaattcca acataaaaaa attgttttta gaataaataa
     3181 tattggcata gtaattgctt cactataaga cacaaagata aaaaattaaa atgacaccga
     3241 aaaatttgaa aggggaatat aaaataaatg agagaaataa aatggaaatt aaataactta
     3301 cctaaatcag aagaaaaaga aaaaggaatt gagtttttaa gagatgaaga aattgcaaag
     3361 gctaaagctt ttcatgagag ctttccacaa tatgaaaaga ctcctttagt taaattagat
     3421 aacttagcaa aatcactagg cgtaagcgga atatatgtta aagacgaatc ttatagattt
     3481 ggtttaaatg catttaaagt tttaggtggt tcttattcta tgggtagata cttagctcaa
     3541 agattagata cagatatatc tgagttaggg tatgacaaat taacttcaaa agaaataaaa
     3601 gaaaaattag gagaaataac tttctttaca gccacagatg gaaatcatgg tagaggagtt
     3661 gcttggacag ctaataaatt aggtcaaaaa tctgttgtat taatgccaaa aggttcttct
     3721 gaatttagat taaataaaat caaaggagaa ggtgcagatg ctagtataac agacttaaac
     3781 tatgatgatg cagtaagatt agctaatgat tatgcagaag ctgatgacca tggagtaatg
     3841 gttcaagata cagcgtggga cggatatgaa gaaataccag catggataat gcaaggatat
     3901 ggaactatgg ctaaagaagc tatagaacaa ttaaaagaat atggagtaga tagaccaaca
     3961 cacgtatttg ttcaagcagg agttggttca cttgctggtg cagttcaagg atatgttgct
     4021 tcaatatatg atgaatgccc aataacagta gtagtagagg ctgatgaggc tgattgttac
     4081 tataaatcag cagaagctgg agatggaaaa ccaagatttg ttggaggaga tatgccaact
     4141 ataatggcag ggcttgcttg tggagaacca aacactatag gttttgaagt gttaaaaaat
     4201 tattcatcag cattcgtttc agcaccagac tgggtatctg ctaaaggtat gagaatatta
     4261 ggaaaccctc taagaggaga tgagaaagta atctctggag aatctggagc agttacaact
     4321 ggagcattag tttcaatcct tgaaagtgaa gatttaaaag acttaagaga agctttgaaa
     4381 ctagatgaga actctaaagt attattaata agtacagaag gagatactga ccctgataaa
     4441 tatagagata tagtttggaa tggtgaatgc caaagtaaat aaaagactta aggaaaagaa
     4501 aatcttaaca aataaatgaa aaatttaagg aaaataaaaa cttaaataag tgaaaacttt
     4561 aaataaaata aaaactaaaa caaatgaaaa atataaataa atgaaaaagt aaagcaaatg
     4621 aaaaattcaa aataaaaact aaataaaatt aaataaaacc aaataaaatt aaaataaagt
     4681 ctaaataaaa atttaataaa taaaatataa agaaataaaa aatcaataga ttaagtgaaa
     4741 tgctaataaa ttaaaaaagt gaaatatcaa aataaaacgt caacaaatta aaaattatat
     4801 gggggaatat caaaatgtta aatgaaacaa gaaaaaaaca attaactgaa gtatgccaag
     4861 acttaataag aaatgctagt tattcaggtc aagaagaaaa cgtagtaaaa gctatagaag
     4921 aaaattttgc tttactaggt tttgatagtt ggtcaagaga tagatatgga aatatagtag
     4981 gatgtataaa aggaaataaa ccaggtaaaa aaatattatt tgacgggcat atagatacag
     5041 ttccagttcc agatgcaagt aaatggagtg ttcctccttt tgaagcgaga atagtagatg
     5101 ggaaaatata tggtagagga acttctgata tgaaggggca agtaagtgct atgatggctg
     5161 ctgtttcata ctttgcagaa gatactaaca aagactttga aggtgaattg tatgtagcag
     5221 gtgttgttca tgaagaaata tttgaaggtg tttcagctag agagataagt aaggctgttc
     5281 aacctgatta tgttgtaata ggtgaatctt cagaattaaa cttaaagata ggtcaaagag
     5341 gtagaggaga aatagtagta gaaacttttg gtaaaccagc tcactctgct aatccagaaa
     5401 aaggt
//
LOCUS       DAEMHW010000222         5348 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEMHW010000222 DAEMHW010000000
VERSION     DAEMHW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.222"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(204..1388)
                     /locus_tag="KPK67_003359"
     CDS             complement(204..1388)
                     /locus_tag="KPK67_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG2460279.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="KPK67_003360"
     CDS             1761..4112
                     /locus_tag="KPK67_003360"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBG2460280.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="KPK67_003361"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="KPK67_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBG2460281.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2091 a          598 c          851 g         1808 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttagtttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttactt tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaattta
      241 tctaatccag ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtatgggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caagattgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagaacat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaatac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actattccct aattttagct
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccggat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgcc ttgaggttca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta ggaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 aatgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga agcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gtttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tatcgataca ttatttaatc
     4861 gattcttatg ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat
     4921 ctagataagt tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt
     4981 gtgaatatta agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac
     5041 taaacacata agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat
     5101 gcaatatcta tctttataaa aaaatattaa attttgtaat ttgtggataa atatgtttat
     5161 aatgtggata atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg
     5221 gataatatta taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt
     5281 tgttgactct aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca
     5341 aataaagc
//
LOCUS       DAEMHW010000223         5326 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEMHW010000223 DAEMHW010000000
VERSION     DAEMHW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.223"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..2513
                     /locus_tag="KPK67_003362"
     CDS             <1..2513
                     /locus_tag="KPK67_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase C80"
                     /protein_id="HBG2460282.1"
                     /translation="KDIKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDED
                     SKLVKGLININNSLFYFDLIESNLVTGWQTINGKKYYFDINTGAASTSYKIINGKHFY
                     FNNNGVMQLGVFKGPDGFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKA
                     VTGWRIINNEKYYFNPNNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDT
                     DTAIAFNGYKTIDGKHFYFDSDCVVKIGVFSGSNGFEYFAPANTYNNNIEGQAIVYQS
                     KFLTLNGKKYYFDNNSKAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTA
                     EAATGWQTIDGKKYYFNTNTSIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTDANNIEGQAILYQNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAA
                     IHLCTINNDKYYFSYDGILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPA
                     NTHNNNIEGQAIVYQNKFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATG
                     WQTIDGKKYYFNLSTAEAATGWQTIDGKKYYFNTNTAVAVTGWQTIKGKKYYFNTNTS
                     IASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQNRFL
                     YLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIG
                     VFKGSNGFEYFAPANTDANNIDGQAIRYQNRFLHLLGNIYYFGNNSKAVTGWQTINGK
                     VYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(2848..3426)
                     /locus_tag="KPK67_003363"
     CDS             complement(2848..3426)
                     /locus_tag="KPK67_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin regulator"
                     /protein_id="HBG2460283.1"
                     /translation="MFFGNISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAE
                     PWFKMKDDEKKAIEAENQRKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLAR
                     TPDDYKYKKVKFEGKVIQVIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDY
                     ITIRGISAGTITYESTMGGNITIPGIAVEKIN"
     gene            3971..4216
                     /locus_tag="KPK67_003364"
     CDS             3971..4216
                     /locus_tag="KPK67_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBG2460284.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(4434..4703)
                     /locus_tag="KPK67_003365"
     CDS             complement(4434..4703)
                     /locus_tag="KPK67_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG2460285.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
BASE COUNT         1984 a          726 c          722 g         1894 t
ORIGIN      
        1 ttaaagatat taaaaaacta tcattaggat atataatgag taattttaaa tcatttaatt
       61 ctgaaaatga attagataga gatcatttag gatttaaaat aatagataat aaaacttatt
      121 actatgatga agatagtaaa ttagttaaag gattaatcaa tataaataat tcattattct
      181 attttgatct tatagaatct aacttagtaa ctggatggca aactatcaat ggtaaaaaat
      241 attattttga tataaatact ggagcagctt caactagtta taaaattatt aatggtaaac
      301 acttttattt taataataat ggtgtgatgc agttaggagt atttaaagga cctgatggat
      361 ttgagtattt tgcacctgcc aatactcaga ataataacat agaaggtcag gctatagttt
      421 atcaaagtaa attcttaact ttgaatggca aaaaatatta ttttgataat gactcaaaag
      481 cagtcactgg atggagaatt attaacaatg agaaatatta ctttaatcct aataatgcta
      541 ttgctgcagt cggattgcaa gtaattgaca ataataagta ttatttcaat cctgacactg
      601 ctatcatctc aaaaggttgg cagactgtta atggtagtag atactacttt gatactgata
      661 ccgctattgc ctttaatggt tataaaacta ttgatggtaa acacttttat tttgatagtg
      721 attgtgtagt gaaaataggt gtgtttagtg gctctaatgg atttgaatat tttgcacctg
      781 ctaatactta taataataac atagaaggtc aggctatagt ttatcaaagt aaattcttaa
      841 ctttgaatgg taaaaaatat tactttgata ataactcaaa agcagttacc ggatggcaaa
      901 ctattgatag taaaaaatat tactttaata ctaacactgc tgaagcagct actggatggc
      961 aaactattga tggtaaaaaa tattacttta atactaacac tgctgaagca gctactggat
     1021 ggcaaactat tgatggtaaa aaatattact ttaatactaa cacttctata gcttcaactg
     1081 gttatacaat tattaatggt aaacattttt attttaatac tgatggtatt atgcagatag
     1141 gagtgtttaa aggacctaat ggatttgaat attttgcacc tgctaatacg gatgctaaca
     1201 acatagaagg tcaagctata ctttaccaaa atgaattctt aactttgaat ggtaaaaaat
     1261 attactttgg tagtgactca aaagcagtta ctggatggag aattattaac aataagaaat
     1321 attactttaa tcctaataat gctattgctg caattcatct atgcactata aataatgaca
     1381 agtattactt tagttatgat ggaattcttc aaaatggata tattactatt gaaagaaata
     1441 atttctattt tgatgctaat aatgaatcta aaatggtaac aggagtattt aaaggaccta
     1501 atggatttga gtattttgca cctgctaata ctcacaataa taacatagaa ggtcaggcta
     1561 tagtttacca gaataaattc ttaactttga atggcaaaaa atattatttt gataatgact
     1621 caaaagcagt tactggatgg caaactattg atggtaaaaa atattacttt aatcttaaca
     1681 ctgctgaagc agctactgga tggcaaacta ttgatggtaa aaaatattac tttaatctta
     1741 gcactgctga agcagctact ggatggcaaa ctattgatgg taaaaaatat tactttaata
     1801 ctaacactgc tgttgcagtt actggatggc aaactattaa gggtaaaaaa tactacttta
     1861 atactaacac ttctatagct tcaactggtt atacaattat tagtggtaaa catttttatt
     1921 ttaatactga tggtattatg cagataggag tgtttaaagg acctgatgga tttgaatact
     1981 ttgcacctgc taatacggat gctaacaaca tagaaggtca agctatacgt tatcaaaata
     2041 gattcctata tttacatgac aatatatatt actttggtaa taattcaaaa gcggctactg
     2101 gttgggtaac tattgatggt aatagatatt acttcgagcc taatacagct atgggtgcga
     2161 atggttataa aactattgat aataaaaatt tttactttag aaatggttta cctcagatag
     2221 gagtgtttaa agggtctaat ggatttgaat actttgcacc tgctaatacg gatgctaaca
     2281 atatagacgg tcaagctata cgttatcaaa atagattcct acatttactt ggcaatatat
     2341 attactttgg taataattca aaagcagtta ctggatggca aactattaat ggtaaagtat
     2401 attactttat gcctgatact gctatggctg cagctggtgg acttttcgag attgatggtg
     2461 ttatatattt ctttggtgtt gatggagtaa aagcccctgg gatatatggc taaaatatat
     2521 gtttgataaa aaattattcc tgtgctactc agaaattatt tttatataat aaatattgag
     2581 atttaattaa aagtcatgtg ttattgtaat acatgacttt tagttaaaat ttttctatca
     2641 tttaataatc tattattctt gactatttta taataaaatt catatatgga aatattaata
     2701 ctaaataatt aatagttgat agaaaataga taatatgcta aaagcaaaaa ctaatttaga
     2761 gccttgtaac tgtttatttg caattataaa aacatcttta aacatattga ctataatata
     2821 aaatattaac tataatacaa aacaatatta attaattttc tctacagcta tccctggtat
     2881 agttatattt ccacccatag ttgattcata agttatagtt ccagcactta tacctcttat
     2941 agttatgtaa tcatcctcta atactcttga aggagttatt gattttttat aactacagag
     3001 tacaacctta tcataatttc cagacacagc taatcttatt tgcacctcat caccatcttc
     3061 aataacttga ataaccttac cttcaaattt tacctttttg tacttataat catcaggtgt
     3121 tctagctaat tggtcataag taataccagt atcatatcct ttcttctctt cttcttcttt
     3181 tttacgttgt tcttcagcct ttttagcttc ttcagctttt ttagcttctt cagctttacg
     3241 ttgattttca gcttcaatag ctttcttttc gtcgtctttc attttgaacc atggttcagc
     3301 atcagacaat ttgctattta aagtttctat ttctgttttt tgagaatcta taactgatgt
     3361 ttgattagaa atgacctcct catggtcttc agaacaagct ggtgaggata tattgccaaa
     3421 aaacacgccc aaaataaatg ccagtagagc aatacccttt gtgctcttaa aaaatttaat
     3481 tattttctta gagcttcctt ttccttcatt actaaattcg ttaccctcat ttttttagaa
     3541 aacataatac aatctccctt ttattagata tattttattt taaaagttaa aaacttaaaa
     3601 taactagttg actaaaaatt atatattcat attttcctaa aatatatttc ccctaagcat
     3661 aattttacaa aataaagtaa gaagattcaa tatattttga aaaaataaga taaaaatgtc
     3721 agattataat ataattttct tgaaggtgaa ttttatatat taaattaaac ataaagtatt
     3781 ttatttgtat aaaactgtat tatgatgtaa ttaagtgact cagtgattta gaggtggtaa
     3841 aatatatata gatgaaagag cataaataaa ttgatataat aaaaaattaa aaattaaata
     3901 ttaactataa taaaaaaata tctggtaata agtataatac aaaaagaata taccagaaag
     3961 aaggtgaagt atgggagaac tacaaaaaat tccaggggta gggaaagcaa cagaaaaatc
     4021 tttaatcatg ttaggatata caactattaa atcacttaaa gatgcaaacc cagcacaaat
     4081 gtatgagaag gaatgtttaa tgcgtggaca gcacatagac agatgtcagt tatatgtata
     4141 cagatgtgca gtatattttg catcaactga aaatccagaa ccagaaaaat taaagtggtg
     4201 gtattggaaa gattaaaaaa ccaaaaaagt tagacataaa aaagagttga atatccgttt
     4261 taaataataa aaactaatac tcaactcttt tttagtcgta aattttatgt aaacctattt
     4321 gtatggctaa atatacccat caataaatgt ataaatatac caatcaacaa ataactaaac
     4381 atactaccta acaaaggatt aaataaatta cttaacaaag cattctaaaa caattaatta
     4441 gaaactgttt cttcaagcca ttttctacat tgctcaatat ttaccatagg gtcaaaacct
     4501 tggtcttttc ccttatttaa aagcatatca caagggaaag tatcacattc accacaatga
     4561 ttaagttcct tactttcaca gcaagtttta accttacatt ctccacccca aaatggtttt
     4621 tccatagttg ggcaaccagt gcaatttact tgttcttttt ttgtacaact gtcacaacag
     4681 acaccacatc ttgattcaaa catgttcaaa catcctttcc attatttaaa aatattataa
     4741 aatgatttta gatgaataca aaagtgcaaa tagttgattt taggtcatct aaatgttaaa
     4801 cataaaatta ttagtactgg taaatttata aaaactattc caaaaatctt tttgaatatt
     4861 tcaaaacaca ataaattact tcaaatccct ttgaatatat tttacagcat tatagattgc
     4921 tttaaatctc tttgaatatt ctacaagact atagattagt ttaaatctct ttgagtattc
     4981 tacaagacta tagattaatt taaatccctt tgaatatttc aaaacaccat agattacttt
     5041 aaattccttt gaatattcta taacactata aattactttt aacttttata taaccataaa
     5101 gtttttatct ggcactttaa atggagtttt atatcaaatt tgccttccat cgaataacca
     5161 aacttaattt aattacacat tccaagagtc aaatattcat tcaatatatt tgataattct
     5221 atgttatagc tataattaaa tatagattat taaagttaaa tatttattta atatattgaa
     5281 tgatttttta cctatattta attaagtttc attatataat ataaaa
//
LOCUS       DAEMHW010000224         5308 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEMHW010000224 DAEMHW010000000
VERSION     DAEMHW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.224"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            14..1897
                     /locus_tag="KPK67_003366"
     CDS             14..1897
                     /locus_tag="KPK67_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBG2460286.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGMIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2122..2802
                     /gene="pgmB"
                     /locus_tag="KPK67_003367"
     CDS             2122..2802
                     /gene="pgmB"
                     /locus_tag="KPK67_003367"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG2460287.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2799..5084
                     /locus_tag="KPK67_003368"
     CDS             2799..5084
                     /locus_tag="KPK67_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG2460288.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNIPVFTVG
                     SMMYIKTNGDVEAISHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADNEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKKCNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKCIKIDK"
BASE COUNT         2197 a          473 c          983 g         1655 t
ORIGIN      
        1 gggaaatttt aaaatgtcta ataaaaataa agaaaatata actttaagta atcaaatcaa
       61 aggaacaatt caaaatagtt taacaaaaga agctatatta catataccaa tgagcaatta
      121 tgcctatggt tatgatagag aaacactaca tataagactt agaactaaaa aaaatgaagc
      181 gaaaagagta attttacgaa taggagacca atatgtgtgg gataaaggcg gtgcaggagg
      241 aggaaattta aatgcatctg gagttggttg gtctggtgga acaaatatag taatgagtaa
      301 agaagtagaa actgaattat ttgactattg gatagctaag tgtaagcctt taaataaacg
      361 ctcacgatat ggctttataa tagaaggtca agaggaaaaa cttttattta cagaaaaaag
      421 aataatagag ttgggaaatg aaaatgatga aaaggaatta tgtgaaatag gtaatttttt
      481 tggatatcca tatttaaatt atatagatat acctaaagtg ccaaattggg tgaaagatac
      541 tgtttggtat cagatatttc cagatagatt tgcaaatggt gacccgtcta taaacccaga
      601 aggagtagat aaatggggag ctattccaac cagagataat tttacaggtg gagatttaca
      661 aggtgtgata gaacacttag attatttgag tgatttggga ataaacggaa tttacttttg
      721 ccctattaca attggaaaaa ccaatcatag atatgatacg gtagattata tggaggtaga
      781 ccctacatta ggagataaag agactttaaa aaaactaata gaagaagcgc ataagagaaa
      841 tataaaaata atgttggatg ctgtttttaa tcatatagga tattattcta aacagtggca
      901 agatgtagtt caaaataagg aaaattctcg atataaaaac tggttttata taaaagatat
      961 gagtaaaatt gatactccaa tagaacaaat agatgaaaaa aatatacctt atgaaacctt
     1021 tgggtgtgaa aaatatatgc ctaaacttaa tacagaaaat tcagaagtaa taaaatattt
     1081 attggatgta ggcaagtact ggatacagga atttgatata gatgcgtgga gattagatgt
     1141 ttcaaatgaa gtagaccatg ttttttggag aaagtttaga gaagaagtca aaaaagtaaa
     1201 accagacatt tatatattgg gagaaatttg gcatggtagt ttaccatggc taatgggtga
     1261 ccaatttgat tcagttatga attacttgat gtcagaagcc atgaaaaaat tcttttgtac
     1321 agatgagata aacgccgaag aatttaagta tatgataaat gatgtaatgg taagttatcc
     1381 aatacaagtt agtgaagtta tttttaattt acttggaagt cacgatacta caagaatatt
     1441 gacttatgct aatggaaata ttgatagatt taaacttgca tatatgttta tgtttgtaca
     1501 aacaggatgt ccttgtatat actatggtga tgaaatcgga atggaaggag aattaagttc
     1561 aatatctgaa ggtcaaagaa aatgtatgga atggaatgaa gaaaaatgga ataaggatat
     1621 atttgatttt atgaagaaga ttattagact tcgtaaagaa aataaagaac ttaggtctat
     1681 atcaaatgaa tgggtgttag cagataagga aaatggaatg attatattga aaaaagaatc
     1741 tatttctata ataataaata attcatctaa aaatataatt ttgacgttac cagattattt
     1801 agcaaataaa aaagtaaaag atttatatga agaagaaata gttgatttaa aagaagaact
     1861 aatgttagag aaatacaagt tcattatttt aaaataatac aaaaaagtat ttattttaga
     1921 tttcatgtta aattattata cagtttaaac aagtctttat aaacgataaa aaaatatggt
     1981 ctacaagtga gtgaattgaa agtagatttt aaattaaaga ctgagttaga gtagataatt
     2041 ttataaataa tatcttaaat acaagataga aaataaattg attattagtc taaaaatttc
     2101 ttgtagaaag aggaaataag aatgattgaa gcatttatat ttgatttaga tggtgtaatt
     2161 acagatactg catattatca ctatatggct tggagaaaac tagcccataa agttgggata
     2221 gatatagaca caaaatttaa tgaatcttta aaaggcataa gtagaatgga gtcattggat
     2281 aggatattag aatttggaaa taagaaatat tcattttcag aagaagaaaa agtgagaatg
     2341 gctgaagaaa aaaataatta ctatgtatca ttgatagatg agataacttc aaatgatatt
     2401 ttgccaggca tagaaagctt gttaattgat gtaaaatcta ataatataaa gataggtcta
     2461 tcttctgcaa gtaagaatgc aataaatgta ttaaatcatt tgggtataag tgacaaattt
     2521 gattttatag ctgatgcagg aaaatgtaaa aacaataaac cacatccaga gatatttctt
     2581 atgtcagcga aagggcttaa tgtaaatcca caaaattgta tagggataga ggatgcaagt
     2641 gcaggtatag acgctataaa ctcagctaat atgttttctg ttggtgttgg aaattatgaa
     2701 aacttaaaaa aagcaaattt agttgtagat tcaacaaatc aattaaagtt tgaatatata
     2761 caagaaaagt ataatgagta tatagttagg agaattatat gaaatgttta tttagtaatg
     2821 tagatgagtg gaagattata caagataaga ttgaaatcaa agaaaataga ttagcagagt
     2881 ccataatgag tataggcaat ggatatatgg gtatgagagg aaactatgag gaaaattata
     2941 gtggagatac acacaagggg tcttatatag ctggagtatg gtttccagac aaaactcgtg
     3001 ttggttggtg gaaaaatggg tatccagaat actttggtaa aattcctaat tcagtcaact
     3061 atataggtgt aagaattttc atagatgatg tagaattaga tttagcaaaa tgtaaagtag
     3121 aagattttca tagagaatta gatatgaaaa atggtatttt aaatcgtagg tttattgtaa
     3181 atataaatga taaaaaattt gaagttaggg ttactaggtt tttaagtata tctgttaagg
     3241 aacttgcagt aattaaatac agtataaaat cattaaattc taatactaaa ataaaatttg
     3301 taccatattt agattcaaat ataaaaaatg aagattcaaa ctatgaggaa aagttttgga
     3361 atggaatttc ttcaagttct ttagaatata aagggagtat tctttctcga acaaaggaga
     3421 atgcatttaa tataccagta ttcactgtgg gttcaatgat gtatattaag acaaatggtg
     3481 atgtagaagc tatatctcat gagagtgatt ataattactg tgaaaattcg ataacaatgg
     3541 atgttttaaa aaatgaggtt gcaagtatag aaaaatatgt atctataact tcaactagag
     3601 attataagga aagtgagcta ttaagaaaat gtgaagacat tttaaataga gaactatcta
     3661 aaagttatga tgatatatta aatgaacata caaagttatg gaataaaaga tgggaaagtg
     3721 cagatgtaaa aataaatgga gataaaagta gccaacaagg tataagatat aatttattcc
     3781 aactattttc aacatattat ggagaagatt cgcgattaaa tataggtcca aaaggattta
     3841 ctggtgaaaa gtatggagga gctacttatt gggatacaga agcgttttgt gttccagtat
     3901 atctaggtgt agcagataat gaggtaacta gaaatctttt agaatatagg tacaatcagt
     3961 tagaacaggc aaaggataat gcaaaaaaac taggacttaa aggtgcttta tatccaatgg
     4021 taacatttga tggagtagaa tgtcataatg aatgggagat aacatttgag gaaatccacc
     4081 gaaatggaag tatagtatat gcaatatata actatacaaa ttatacaggt gactataaat
     4141 atataaaaga aaaaggaata gatgtaataa tagaaatagc tagattttgg gcaagtagag
     4201 ttcatttgtc tacaaaaaaa gatttgtata tgatacatgg agtaacaggt ccaaacgagt
     4261 atgaaaacaa tatcaataat aattggtata ctaattatat agctaaatgg tgtttagatt
     4321 atgctgtaga aaatatctta cgattagaga aaaagtgtaa tgaatgtatt gacagaaata
     4381 atgtgactag ggaagaagtt gagttttgga aaataatatc aaataaaatg tatctgccgt
     4441 atgatgagga actacaaata ataattcaaa atgataattt tttagataaa gaatttgtag
     4501 aagtaaagga tttaccaaaa gaaaattttc ctcttaatca aaattggtct tgggataaga
     4561 ttcttaggtc gtgttttata aaacaagcag atgtattaca gggaatttac tattttggaa
     4621 atagatttac taaagaagaa aaaaagagaa attttgactt ctatgaaaaa tatactgtcc
     4681 atgaatcttc tttatcttct agtatttatt ctattattgc ctcagaaata gataacctag
     4741 aaaaggctta tgaattatat tcaagaacag ctaggcttga tttagataat tacaataatg
     4801 atacaaatga tggattgcac ataacttcaa tgtctggttc atggctttcc attgtacatg
     4861 gatttgcagg catgagaaca tggaatgaaa ctctatcttt tgaccctaag ttgccaaaag
     4921 agtggaaaaa ttattctttc aatataaatt atagagataa tattattaat gtagatgtat
     4981 gtagtgattt aattaaaata gaaaatttaa aaggtaaacc aataaaaatt attgtatatg
     5041 ggaaaaaata tatattagaa aaatgtataa aaattgataa atagatttaa taatattttt
     5101 attatataaa tagttaaaat aagcttatct aattatttat ttatttattt attttaactg
     5161 ttttgtgtga tagaaaacta tatattataa caatagtagt gcttttaatt aaatatattt
     5221 cataaatctt cataaaatct tcaaaatttc gtaaagaata tgtcacaaaa gtaaggtata
     5281 ttatattcag cagatagtat aagggagg
//
LOCUS       DAEMHW010000225         5257 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEMHW010000225 DAEMHW010000000
VERSION     DAEMHW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.225"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..423
                     /locus_tag="KPK67_003369"
     CDS             <1..423
                     /locus_tag="KPK67_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2460289.1"
                     /translation="ARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNI
                     IRNRKLSRLISDVSWSEFIRQLEYKANWYGRKIVKIGKFFASSQICNKCGYKNEEVKD
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            541..1227
                     /locus_tag="KPK67_003370"
                     /pseudo
     CDS             541..1227
                     /locus_tag="KPK67_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(1448..2485)
                     /locus_tag="KPK67_003371"
     CDS             complement(1448..2485)
                     /locus_tag="KPK67_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG2460290.1"
                     /translation="MNKKLIKAKIFIWSILAISLSIVFVSLLIGTIISHSFNFPFNTL
                     NPKYNYDNYIVAKNESFSDKINDINVDWISGEVRIKRSDSNKIKLIQKIPSNFSKKKL
                     AKIMVDENSLSIYDNSTKKFFIGINLPKPTILELYLPNKLYDKITLYTTSADIYSTYI
                     RSNIVEANTISGAIGLSGNINKINLDTTSGHIDITKLNSKNATLKSTSGEIQLSGKID
                     VLNLETTNGNINLKDIYNKNLTCSSVSGKMNLSGVFSDIKAESTSSNIKINSSKMLSS
                     LYCETTSANVDLSIPDNPGFTLNFDKVSGVLDSDFELNRNSDLYTYKNGITDLYLSTT
                     NGNLTIIKNNH"
     gene            complement(2478..3164)
                     /locus_tag="KPK67_003372"
     CDS             complement(2478..3164)
                     /locus_tag="KPK67_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460291.1"
                     /translation="MLDKIRTYIDSSFTGVNETKKVKELKDELFENLKEKYNDQIQDG
                     KTEQEAYNSVISGIGDLSELIESVKEPYSLPSELIEEKKKRALRASIAVALFIISPFL
                     FVVSVVSFGIEPTIAILLMLILIALGVGLLVYNQMTRTNYALYNDNLSNKFKEFEDFE
                     ENYNHINPVYKSFMNAYWIIIVSIYLITSILFNIWSFSWILFIIGAAGVNIVKGIIQL
                     KGYKEEDKYE"
     gene            complement(3176..3496)
                     /locus_tag="KPK67_003373"
     CDS             complement(3176..3496)
                     /locus_tag="KPK67_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2460292.1"
                     /translation="MIIASDMIRGHTETIILKHLLEKDSYGYEINKSILEKTKGLYEL
                     KEATLYTAFRRLEQSGAITSYWGDEVTGARRKYYSITNIGKEKYIKSREEWNNTKALI
                     DILI"
     gene            3857..5197
                     /locus_tag="KPK67_003374"
     CDS             3857..5197
                     /locus_tag="KPK67_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2460293.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPESGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKINKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         1973 a          570 c          793 g         1921 t
ORIGIN      
        1 gcaagattaa aagttgcaag acttcaagaa catatagcta atcaaagaaa tgattttcta
       61 caaaaactat ctactaaatt aataaaagag aatgatatta tttgcataga agatttacaa
      121 gtaaagaata tcattagaaa tcgtaaacta tctcgcttaa tttcagatgt atcatggtca
      181 gaatttattc gtcaattaga atacaaggct aattggtatg gtagaaaaat tgtaaaaata
      241 ggtaaattct ttgcaagttc acaaatatgt aataaatgtg ggtataaaaa cgaggaagtt
      301 aaggacttaa atataagaga atggatttgt cctagttgta atgaaactca cgatagagat
      361 ataaatgcaa gtataaatat attaaaagaa ggattaagac taataacaat tcaaaataaa
      421 taacgtatat aagaaccgta ggaactacgg ggatagcctg tggagaatta gtaagacata
      481 ttttagtatg caaaattcta tgaagcagga accctgtgac tttagtcatg ggaggttcag
      541 attttggctg aagatgaaga aaataatcat ataaatattg agatgcaaag agcttttgag
      601 aacgattatt tggaaagagc tgaatattat cttagtcgtg tacatggtag aaagttagaa
      661 gaaggtaaag agtataaaga aattggaaaa actattggaa tacatatact aaatcatgta
      721 aaatataatc acatagaaga ttatgtaaat tgcctgagat taacaatgga cgggcatcca
      781 gatatttatt ctagtaaaac tgcattatat tttatagagc ttccaaaaat tcataaatct
      841 gactatatag taaatagaat tatgctatgg ggaaaactta ttagtaatcc ttctagctta
      901 gatgtaagag ttatagctaa gaatgattct attattaaaa aagcacttga tagattaaaa
      961 gaattaggta gcaatgaaga atatttatta aatctgaaaa gaggtgctta tatcatgaat
     1021 aaaagtagaa attttaaaga agagattgaa agagaaacgg aaacaagagt tgcaagagag
     1081 acagcaatta gagttgcaaa agaaaaagat tataaaataa ttataatgtt gaaaaaaaat
     1141 ggttttaaat tagatgacat attaaataaa tttaatgatt ttgacttaag tgaagatgag
     1201 ataaaagaag tatatgaaaa taattgatga taaataaaaa gtgaacttat ataagtttgc
     1261 tgaatcggag tctggaaata tttatgtgta ttttttaaat gaatgaaaat ttattttaaa
     1321 aataataatt tagaggtaca gttaattata tttttatgat actgtacttc tagttttttc
     1381 tattgtagat ttattattta ctaataaatc tacaatagaa gtgtttttgt tgtaagaatc
     1441 attttatcta atgattgttt tttataatag ttaaatttcc atttgtagtg cttaaatata
     1501 aatctgtaat accattttta taagtgtata aatcactatt acgattcaac tcaaagtcac
     1561 tgtccaaaac accagaaacc ttatcaaaat ttaaagtgaa acctggatta tcaggaatag
     1621 acaaatcgac attggcagaa gtagtttcac agtacagaga agacaacatt ttgctagaat
     1681 tgatttttat attactgcta gttgactcag ctttaatgtc agaaaaaact ccagataagt
     1741 tcatttttcc agaaactgaa ctacaagtaa gatttttatt gtatatatct tttaaattta
     1801 tattgccatt agttgtttct aagtttaaaa catcaatttt cccagaaagc tgtatttcac
     1861 cagatgttga tttgagtgta gcatttttac tatttagttt tgttatatca atgtgaccac
     1921 tagttgtgtc taaatttatt ttattaatat taccagagag acctattgca cctgaaattg
     1981 tgttagcttc cactatatta cttcttatgt aagtgctata tatatctgct gaagtagtat
     2041 ataaggttat tttatcataa agcttattgg gcaaatatag ttcaagtatt gttggttttg
     2101 gaagattaat tccaatgaag aattttttag ttgaattatc atatatagaa agagaatttt
     2161 cgtcaaccat tatttttgct agttttttct ttgagaaatt actagggatt ttttgaatta
     2221 atttgatttt attagaatca cttctcttaa ttctaacttc acctgaaatc caatcaacat
     2281 taatgtcatt tattttatct gaaaaacttt catttttagc cactatatag ttatcgtaat
     2341 tgtatttagg attaagagta ttaaaaggga aattaaaaga atgtgaaatt atagttccta
     2401 taaggagtga gacaaataca atagaaagtg atattgccag gatactccag atgaaaatct
     2461 ttgcctttat aagcttttta ttcatattta tcctcctcct tgtagccttt tagttgtata
     2521 attcctttga caatgttgac accagcagca cctattataa atagtatcca cgaaaaggac
     2581 caaatattaa atagtatgct tgtgattaga tatatagaaa ctattataat ccaatatgca
     2641 ttcataaatg atttataaac tggatttata tgattataat tttcctcaaa atcctcaaat
     2701 tctttgaatt tatttgataa attatcattg tatagagcat agtttgtcct tgtcatttga
     2761 ttatagacta ataaacctac tccaagtgct attaatatga gcattaaaag aatagctata
     2821 gttggttcta tgccaaaact tacaactgag actacaaata ggaaagggct tattataaaa
     2881 agagcaactg ctattgatgc tcttaaggct cttttctttt tttcttcaat taattcagaa
     2941 ggtagagaat atggttcctt aacactttca attaactcag ataaatcacc aattcctgag
     3001 attactgagt tatatgcttc ttgttctgtt ttaccatctt gtatttggtc gttatacttt
     3061 tcttttagat tttcaaataa ttcatcttta agttctttga ctttttttgt ctcattaact
     3121 cctgtaaagc tactgtcaat ataagtacgt attttgtcta acatattttc acctcctaaa
     3181 ttaatatatc tatcaatgct tttgtattat tccattcttc acggcttttt atatatttct
     3241 ctttacctat gtttgttatt gaataatact ttcttctagc tccagtaact tcatcacccc
     3301 aataagaggt tattgcacca gattgttcaa gtctacgaaa tgctgtatac aaagtagctt
     3361 ctttcaactc atacaatcct ttggtttttt caagtataga tttattaatc tcataaccat
     3421 aactgtcttt ttcaagtaaa tgttttaaga taattgtttc tgtatgacct cttatcatgt
     3481 cagatgcgat tatcatgaac atcacctcca attaaatata taatactttg atatacttcg
     3541 cctgtaaagg tatatcatga gaaaagacat tttattgtaa tatttataag attttatata
     3601 gaaataatgt aaaaacttta taaataatat attttatacc tagttataga taaatttata
     3661 tagttattat taaaaaaagc aataaaaagt ttaattcaat ttagcaaatt tagtgatata
     3721 attaaaaaag aaaagaaata tggacttatt tggaataatt ttgacaatct atttactaca
     3781 cgtgtagagt cgtatataat taaattatac ataaaatata aaaaaataat ttagtaatac
     3841 gttaggagaa agtattatga taaagtcaag cttattttgg aatattttca ttgcgatatc
     3901 ctttatgata gaatggtttc tttgtaagaa aatgctagat tatacaagtg agaaaaggat
     3961 ttctaacaaa aaaataaact tttgtatggt aggtattatt gtttttgctg acataatata
     4021 tttttttgat gtacctccaa atataagagt aattatatct atgataatta ctattgcatt
     4081 ttataaaatc tcatatgatg tggggtttat aaagtctgta ttaatatcct taatttattg
     4141 gatgttacta atcgccacag atgctttgag tatatcttta acagtttggc ttaattctat
     4201 aaatgacatg agtagtatgg ggacgggaga tatatacaga attgagtcta taatattggg
     4261 aaagatactt ttgattgtgt ctttgctttt atatagagcc ttaaagataa aacttaatat
     4321 agaaataaat aaaaggacat tattttatat agttattcct atagtcgcta atatagcatc
     4381 tttttttgta atttttgaat acatatttaa tccagagtct ggtagcttaa tacataattt
     4441 ccaatttgtt atgatttctg ttgtattgct tatatcaagt atcactttag ttttaattgc
     4501 taaaaaatta cagcaatata atactttagt tgtagaaaac aatatgatta aaaatacttt
     4561 gaagtatgaa aatgagtatt atgaagatat agaaaagaaa tatttaaaaa ctagattact
     4621 aagtcatgat atgaaaaatc acatattgtg tataaatgct atgattaaaa aaggtattga
     4681 tgttacttct tatttaaata atttgcaggt agaaattcag agtaatgact tgttatttaa
     4741 tactggtaat tttatattag atgctatatt aagtgaaaag aaagaggttt gtgataagaa
     4801 tgatatagaa ctaaaaatag gaataaattt tagttgttgt gaagctgttg aattagtaga
     4861 tatatgtcat atattttcaa atattttaga caatgcaatt gaggcatgta ataaaattga
     4921 tagtggaaat agagttattt gtgttaaagg tgatgttatg caaaattttt atttaataag
     4981 agttgaaaat actaagataa ataagattat tactaaaaat ggagatattt tgacagacaa
     5041 aaaaggttct cttcatggac ttggaataaa aagtgtaaaa aactctgtaa ataagtatgg
     5101 tggtaatgtt gtaattgaat atgatgataa aaaatttgta gttaaaattg ctattccact
     5161 tagaaatgtg aagaaagcag ttaatcatgt gatttaaatg tatataaatt tgatgtgtag
     5221 aaatttaata tataagaatt aatgtataaa agtgatg
//
LOCUS       DAEMHW010000226         5238 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEMHW010000226 DAEMHW010000000
VERSION     DAEMHW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.226"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            62..679
                     /locus_tag="KPK67_003375"
     CDS             62..679
                     /locus_tag="KPK67_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460294.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            1040..2233
                     /locus_tag="KPK67_003376"
     CDS             1040..2233
                     /locus_tag="KPK67_003376"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG2460295.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            2251..3543
                     /gene="lysA"
                     /locus_tag="KPK67_003377"
     CDS             2251..3543
                     /gene="lysA"
                     /locus_tag="KPK67_003377"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBG2460296.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            3760..4419
                     /locus_tag="KPK67_003378"
     CDS             3760..4419
                     /locus_tag="KPK67_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2460297.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(4574..>5238)
                     /locus_tag="KPK67_003379"
     CDS             complement(4574..>5238)
                     /locus_tag="KPK67_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG2460298.1"
                     /translation="NLLKEASNFVGKIPKNFLIYTLSISSMLFNMTMGFILSIYIIYD
                     KEKIAFGFKRFLYSSTARNKADNIIEFFRTTHDIFYDYLLGRILDSLIIGIIAFLGFQ
                     FVIRIENALFLASIIFLGNIIPYFGPFIGAIPPIAMTILYSPQKTIWVIAFLFILQQL
                     DGNFIEPKVMGNQVGIGAIWVISAILVGQSLFGFIGVFLSVPIAAVVKTYVDKYIDNR
                     LQ"
BASE COUNT         2133 a          551 c          931 g         1623 t
ORIGIN      
        1 gtcataaaat taatataata ttagtacaac tttgcaaata atacttcaag gagaatgtac
       61 tatgacgaat atggaaaaag agatattact tttaattagc agggttagat gtgttacaga
      121 gtctcaattt agaaaaattt atggagacca aaaaagatac aataaaaaaa attttaaaaa
      181 aactttgaga aaaatgtgta gtgaatatac actaaaaaaa tatccttgtg atgtggatta
      241 ttataattat aaagacctct cttatgtata ctatttaaac ggtagtaaac tttttaaggg
      301 agatgattta ataaaggctc tagtaggatc agaattagtt gttagaatca atactgcagg
      361 ttgtgatgta aaaagatttt accgaaatgt tagtgtagat gatgtgaaat acgatttatt
      421 tattgaatat acagaccaat ttggtaatat aaagcaagtt ttagttgatg ttgacttaaa
      481 taatgaaata gacatttcta aatacgaaaa tctttctaaa aaaatagata aatctacaat
      541 tccatttttt aaagtaccaa acgttttggt agttacacca gataatctat ccgaattaaa
      601 tgatttagag agatatgata atgagtatag cataaatttt atagatttag gattaaataa
      661 actatttaat tgtatataag ctaactttag aaaagaaaac tatcgatttg ccacattttg
      721 taagataatt gttgcaatat tatatagaat atgataaaac tatattaata aattaattaa
      781 aaataatgag cctagggtag aggtagctgt attcaatagt attcaatagg agctggtaag
      841 cgttgataat tgatgaaagg gtatatagcc gaagaaattt tgttgacgga caaattttct
      901 ggacttatgc agaatatgca taaggctgtc acttttaaag tggtgagcta caaggttact
      961 tggtctatga aatttacacg tccaaagtat acctttgggc gtttttattt atcaaaattt
     1021 agcttgaggg ggagaaagca tgagtataat tgttcaaaaa tatggtggaa gttcagtagc
     1081 agatactgaa aaaataaaat caatagcaga aaatataata gaacgaagaa aagaaaatcc
     1141 acaaatggta atagttgtgt ctgccatggg aaaatccact gatgaatata taactttagc
     1201 aaaggaattg agtaatgaac ctagtaagag ggaattagat gctttaatgt ctactggtga
     1261 aatgatttca gcatctttat tatcaatagc attaaatgca cttggatgta aagctataag
     1321 ttataatgct tatcagttaa atataaaaac gagtggtctt catggaaagt ctcaaattga
     1381 tgatataaac gtaaacagaa ttaaaaatag tttagatgaa ggaaatgtcg tgatagtaac
     1441 tggatttcag ggaatcaacg aagatggtga tgtaaccact cttggaagag gtggttcaga
     1501 tacatctgct gtagctcttg cggttaaatt aaatggaaaa tgtgagatat atacagatgt
     1561 tgatggaatt tattttacag acccaagaaa atattcaaag gctagtaaac ttgatgagat
     1621 tgaatatgaa gaaatgcttg agttagcgag tttaggagca caagtaatgc attcaagaag
     1681 tatagagctt gcacaaaaat atggtgttga aatctatgtc ggacgtacat gtggtacaga
     1741 aaaaggaaca tatataagag ggggaaaaga catgaaatta gaggataaag taataacagg
     1801 attagctact agtgatgatg acagttctat aacaataaaa gattttaaag ctgaaaatat
     1861 atctagctta tttgaagata tagcaactat aggtataagt gtagatatga taagccaaac
     1921 tgcacctatt ttagataaga taagtgtatc atttacagtt ccaaaagaag agttgggaga
     1981 atgtaaaaag atagtatcta aatatacaga tgaagaacat gtagtgattg ataataatat
     2041 cactaaattt tctttagtag gtcttggtat gaaaaatact tctggagttg ctgctaaagt
     2101 atttaagata ttcaatgaaa atggtataat gataaaactt ataactacat cagagattcg
     2161 tataacttgt gctataaatt ctgatgataa gcaagtagct atagaaaaaa tagcagaagt
     2221 atttaatata taaaatttgg ggaggctatt atgaaacttc atggaactat gactgtaaat
     2281 aatgatgaat tatatattgg aggagtgagt tgtttagaac ttagtaaaaa atatcaaact
     2341 ccactttatg tatttgatga agctttagtt agacaaaatt gtagagaata tatagaatat
     2401 tttaaggcta gagagggaaa aaataaggtt gcttatgcag ggaaagcttt tttacctctt
     2461 tacatgtgta atcttataaa tgaagaacaa atgtgtttag atgtagtttc aggaggcgag
     2521 ttatatacag cttataaatc aaattttcct atggaaaaag ttttctttca tggaaacaat
     2581 aagactaaag aagaaataaa catgggatta gatttaggtg taggtaaatt tgtagtggat
     2641 aatttttatg aattagattt gcttgaagca ttatgtgaag ataaaggtaa aactcaagag
     2701 atatacttta gaataactcc tggtatagaa gctcatacac atgattatat aaaaactgga
     2761 cagatagatt ctaaatttgg atttgcttta gtaaatggag atttatttag agcaatagaa
     2821 aaattaagtg aatataaaaa tattaaactt gtaggtcttc acgctcatat aggttcacaa
     2881 atatttgatg tagaaccata catagataca gtggatataa tgatgaactt ggtaaaagaa
     2941 ataaaagaaa aattcaacat acaactaaca gaaattgacc taggtggagg agttggcgtt
     3001 tattatagcg aaggagataa acctaaaact ataaaagagt tttgtgaaac cataatcact
     3061 aaagcagaaa cttcatgtaa agagcttgga atagaagtac ctacattggt aatagaacca
     3121 ggaaggtcaa tagttgcaaa tgcaggaagt acattgtata cagtaggttc tataaaagat
     3181 ataaaagatg ttagggttta tgtaagtgta gatggaggta tgacagataa tgtaagacct
     3241 tcgttgtacc aagctagtta tgagtgtagt attgtgaata aaattaatca tgaggctatg
     3301 aatcatgtaa caatagctgg aaaatgttgt gagagtggag atatattaat tggagatata
     3361 aacataatgg atattaaaag tggagatata cttataacaa catcaactgg tgcatatgga
     3421 tattcaatgt cttcaaatta taataaaata ccaagagcag cagtagtatc agtatttgat
     3481 ggaaatgata agataatttg taagagacaa agctatgagg acttattagc tttagagata
     3541 taaaatagag aagtatataa taaaaattaa atattaaagt ttattatgag cagatttgct
     3601 aggatatgta tttctaaatt aatttttggt ttattgtatc gttcgtagca tatctgctta
     3661 ttttttgttt tactaacaaa tttcttgtaa taaatataaa atattttaaa aactttataa
     3721 ttatgataaa atttaaagta tatgacaagg agggatttaa tgagaggaag agtaatttta
     3781 ttaatatcga tattatctat atttcttgta ggatgttcat ttaataaaaa agatgaaata
     3841 aatttagtag agcaaggaaa aacatattta gaaaaacata actataaaaa agctatggaa
     3901 tcattatcct ctgcactgga agaagatagt actaatgaga atgcaagagc tatgtatatg
     3961 caagctatga gaatgtcaaa tatgacggag tttgaagaat taaaaaatta tgagggtgca
     4021 atcaaggaac ttaaacttat agagaaaatc aaaaatggtt cctctgttat taaagaagag
     4081 gcaactaaaa agaaagaaga gttaactaaa ttagaaaaag aacaaaacga agcagaggaa
     4141 caaagaaaga aaaaggcaaa agatacggct ggagaagata ggtatagagt tgaatcagat
     4201 gcaaggaaat cttcttatag tggaagtagt aaaaataata aatctagttc taaaaacaat
     4261 gataaaaaat cagatagtaa taagtctgac aataataaca gttcttctca aaatcaagaa
     4321 aagccaaata atagtaatcc aacaccagca ccaactccac aagatactac tggtggttca
     4381 gatagtggtg gaaattctca aaactcaaat aatcaataag tataaaatat atgcataaat
     4441 ttaatgaaca aattatatat tataaaaaat atctcttagc aattttttct tactaagaga
     4501 tattttttga gaaataattt aataaatagc agttaaattt taaactaaaa attaaagtca
     4561 cagtagagat aatctattgt aaacgattat ctatatattt atcaacataa gtttttacaa
     4621 cagcagctat tggtactgaa agaaaaactc ctatgaaacc aaatagagat tgtcccacca
     4681 aaatagcaga tattacccat atagctccta taccaacttg attacccata acttttggtt
     4741 ctataaaatt tccatctaat tgttgcaata taaatagaaa tgcaatgacc catatagttt
     4801 tttgtggact atataatata gtcatggcta ttggagggat tgcaccaata aaaggaccaa
     4861 aatagggaat tatattacct aagaatatta ttgaagctaa aaataaagca ttttctattc
     4921 tgataacaaa ttgaaatcct aaaaatgcaa ttataccaat aataagagag tctaatattc
     4981 taccaagtaa ataatcatag aaaatatcat gagtagtcct aaaaaattct ataatgttat
     5041 ctgccttgtt tctagcagta gaagaatata gaaatctctt aaatccaaat gcaatttttt
     5101 ctttgtcata tataatgtat atggatagta taaatcccat tgtcatatta aatagcatag
     5161 aagatatact aagtgtatag attaagaagt ttttaggaat tttaccaaca aaattacttg
     5221 cttcttttaa taaattat
//
LOCUS       DAEMHW010000227         5186 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEMHW010000227 DAEMHW010000000
VERSION     DAEMHW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.227"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..397
                     /locus_tag="KPK67_003380"
     CDS             <1..397
                     /locus_tag="KPK67_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2460299.1"
                     /translation="IKFTCSINFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENIN
                     KYIRIKGTITRSFFVLKCENSKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYN
                     GELLFENSIDKFILNIYIPLDQDTDSWGD"
     gene            638..1327
                     /locus_tag="KPK67_003381"
     CDS             638..1327
                     /locus_tag="KPK67_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460300.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            1548..3920
                     /locus_tag="KPK67_003382"
     CDS             1548..3920
                     /locus_tag="KPK67_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBG2460301.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDERTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAEGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPDEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            3935..4159
                     /locus_tag="KPK67_003383"
                     /pseudo
     CDS             3935..4159
                     /locus_tag="KPK67_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
     gene            4818..>5186
                     /locus_tag="KPK67_003384"
     CDS             4818..>5186
                     /locus_tag="KPK67_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2460302.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNNKETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMY"
BASE COUNT         2100 a          556 c          941 g         1589 t
ORIGIN      
        1 aattaaattt acttgctcta taaatttttc aaaagtaaat tttgtaaaac ctatagatgt
       61 gtcaagcata ttttctaata ttttggataa tgcaatagaa gcatgtgata aaatagtgga
      121 tgaaaatatt aataaatata ttagaattaa aggaactata acaaggtcat tttttgtact
      181 taaatgtgaa aatagtaaac taaatcagct tacttttaat aaaaatatat tgttaacaaa
      241 caaaatggat aagttcgtac atggaatagg tattcagagt attaaatctt ctttacaaaa
      301 atataatgga gaattattat ttgaaaatag tatcgataaa tttatactaa atatatatat
      361 accattggac caagatactg acagttgggg cgattaagtt accagttggg acatatgtat
      421 tgatacaaaa aaataaggat gttatctttt aataacaaaa gataacatcc ttatttctgt
      481 attaaggaac ttataatttt actaatagta agttataaga attggatagg aaggtgatat
      541 gtattgaata ataaatcttt aatataaaga aaatataaat atattctagt gcaatgaata
      601 atactttaga gcggttagtg tgatgggagg attgtgtatg aaagtaaaaa ggaatataaa
      661 tagagtggca ataattgtgg ctttaaatct tagcttagca ttgatggtag gatgtatgtc
      721 tcctgtatct gataagaact taagtagctc agaacaaatt agtaaagatt cattgaaaga
      781 acaagttgaa gataaaaaag atgaatctat cgagctattt aataattact cacaaaagat
      841 taatgatgcg aaaggtagag aagaagtaaa aaaagattta tctcaaagat tttcgaaagc
      901 tataatagac agtggttata gtattactga ctcaaaatca gatggtagct ttgtagtatc
      961 tataaatggt gctgaaaaac cacctgaaaa aaatattcga agtttattct attctatgtc
     1021 tgaagacgat aaagaaggaa aagtaacaat agatattctc tgtgctaaag agtatccaga
     1081 agatgataag ttatctgaga gtgataaata tattaaattt atatacaatt tatttaaatc
     1141 attgactaat acgaacttaa ctgagaaaga aattttttct atggttgaag aagactttaa
     1201 taagggagta ggtgttgtag agcttcctta tatgaaggat atacatgttg aagttaacaa
     1261 agttaaacaa tcaactaagg ttttaaaact aagtctagta tacaaatttg atatttcaaa
     1321 tcattagtat attagaaata actataacaa tatagaaaat atatcataga aaattttttt
     1381 agattttata aatttaacaa aaaaagtatt aatatatagg tgttcaaggt agatgaaaaa
     1441 aaataagtta ttttgcttcc attttatgct aaaggacagt ataatataat taaagtatgt
     1501 tataacatac aaaaatgtag tatatattat ccaaaggggg attttaaatg agtcaaacta
     1561 atagaataga agcatttaga caagaatata ttaattctaa acctatgata tgttgtgaaa
     1621 gggctagaat ttttacagaa tcacataaaa aaactgaggg agaagctatc tgtataagaa
     1681 gagctaaagc ttttttggag acatgtaaag aacttcctat aaaaatcttt gaaaatgaat
     1741 taatagtagg tacagcagga aaatttagaa gaacagggat attgacacca gagttttctt
     1801 ggcaatgggt agacaaagaa atggatactt ttgatgaaag aactcaagac ccatatgtaa
     1861 tatcaaaaga acaaatagaa tttataagaa aagaaatatt tccatattgg aagggaaaat
     1921 ctttggaaga agttttctta gcaagaatcc cagaagatac agctaagata ttagtagata
     1981 caggaataat agataatgat tctaagtgga gacaggcagt tggtgaagta acaccagatt
     2041 atcaagatat attgtttgta aaaggatata aagggattaa ggaagatgca gataaaaaaa
     2101 ttaaagaatt agatatatca gtttcagaaa atattgaaaa gatagatttt tacaaatctg
     2161 tttctatagt tgcagagggt attatgacat tggcacaaag atattcaaat cttgcaaaag
     2221 agatgagtaa gcaagaaaca gatgaaaaga gaaagttaga acttataaaa atttcagaga
     2281 tatgtatgaa tgtaccagca aaccctccaa ctaattttta tgaagcaata caatttgtat
     2341 ggtttgttca attaggtggt attctatcag aaaatccatt ggcattaaat ttaggtagat
     2401 ttgaccaata tatgtatcca tattatgaaa atgatgctag agaaggtaaa ataactgaat
     2461 cagaagctca agaattgata gaagcacttt ggattaagtt atcagaatgg gtatggacta
     2521 tatcagcaaa tacagcaaat tactttgcag gatataatca attccaaaat cttactgttg
     2581 gtggtaaaaa gagaaatggt actgatggaa cgaatgatat ctcatatatg tgtttaaagg
     2641 ctacagaaag tgtaaaaact caccagccag gattaagtgt tagggtgagt caaggagcac
     2701 cagataattt cgtaatggca gttgctaaat tggtaaaaca aggaactggt ttccctgcta
     2761 tacacagcga tagtgcaggg gcacaaatgt tattacaaga tggatatgat gcagaagatg
     2821 ctagagattg gagtaactgt ggttgtgtag ttcctcattt tagaaaaaca ggacagtgga
     2881 cttcagccgt aaatatcaat tttgcagcag ctttagaata tgctatgaat gaaggtaaga
     2941 gtagattaac tggagaaaaa atggggttag atacaaaaaa tatcacagaa tttacttctt
     3001 ttgaagaact taaggatgag tttttaaagc aattagcata tcttgtaaaa agttctgtta
     3061 taggaactac tgttgctcaa caaattcata aagaaatggt accaagacca tttttatcta
     3121 cttgtgtaga tggatgtttg gataagggag ttgatttaag taaaggagga gcaaaatata
     3181 atataggtcc tgtattaact ggtatagggt taggtgtagt ttcaaattca ttggcagcta
     3241 taaaaaaatt agtatttgag gataaggtga ctacgttaga agaattaaca aaagcactaa
     3301 ataatgattg ggaaggttat gaagaattaa gaaaacttgc gttggatgtt cctaagtatg
     3361 gaaatgataa tgattatgta gattcattag caattgaagt ttctgatttt tattatactg
     3421 aaactagaaa atacaaggat atttttggct ctaaatttaa tagtgcattt atgggtatat
     3481 caaactatgt accaactgga aaaatcgtag gtgcaactcc ttgtggaaga aaagcgacaa
     3541 aacctttgac agagggagtt tctccatttg ttggtactga tacaacaagt ccactagcag
     3601 caatgaaatc tgcatcaaaa ataaatcatg atgttcatac tggtggaaca cttctaaatt
     3661 taagactgaa tcaagatttg gtggaaactg aaagaggact tagaaatcta acatctatga
     3721 ttaaatcata ttttgcttta ggaggattcc atgtacaatt taatacgata tcaaatgata
     3781 ctctattaaa agctcaagaa aatccagatg aatataaaga tttattagtg agagttgctg
     3841 gatatagtac tcaatttgtt aatttatcaa gagaaatgca agatgctata atagctagaa
     3901 attcacatag caatttttaa ttatgaggta gattatgagt aaaaaaggta gagttgtaaa
     3961 agtacaacat ttttcagtaa atgatggaga tggaataagg actactatat ttttagaagg
     4021 atgtaaatta aaatgtaagt ggtgttctaa tccagattcc tggagtaaca tagtaaaatt
     4081 aggtgttatg aaagataaat gtgtatcctg taatagatgt atagatgttt gtcctcaaaa
     4141 tataagttca ctatttgatt aaaaaatagc tacgcacact cgtgacttta gtcatgagtt
     4201 agtagctaaa atagtcagca tatagggaaa cctatatgta gagataggat agaacctatc
     4261 caatactact tgaattgctg gaaaccccta aagctaatca aactaaaaca taaggatgaa
     4321 ataaacctaa gtgtgaaagt tacgaaagta gaaaaaattg gttagatggt ataaggttaa
     4381 atcctaagta ctttaaaatg ggcaatcagc aggtaaactc cgaataggag aaacttcaac
     4441 gactattcct cttgagggaa gtacactaca agctattggt agtggaagtg ggtagacctt
     4501 aacggataat gctgaaggat aagatatagt ctgtgcttac atgaaagtgt aagaagttca
     4561 taagagaact gcataggtag tagcgaactt atgtgaacga caacctcaaa aatgataaaa
     4621 gttaagactt acggttctta aatatgttat ttaaaattaa atataaaacc ttctaaataa
     4681 ttgttttaaa taattacatt tgttaacatt attataggtg attaataaat tgttcaccac
     4741 agcattatat aatatattaa atgtgataaa atgtgataaa atcattaaaa agtgtatcaa
     4801 aaaacaatta aagtgaggtg atagcagtga aaaaagctta taagtttaga atgtatccaa
     4861 ataaaaaaca acaagagtta attaacaaaa cttttggatg ttgtagattt gtatataata
     4921 agtatcttgc taaaagaata gatgtttata aaaacaataa agaaactttt acttataaac
     4981 aatgtagttc tgatttaacc aatttaaaaa aagaattaaa ttggcttaag gaacctgaca
     5041 aattttcact acaaaacgcc ttaaaagatt tagataatgc atataaaaaa ttctttaaag
     5101 aaaaggcagg atttcctaaa tttaagtcaa agaaaattaa tagattttca tataaaacta
     5161 actttacaaa tggaaacatt atgtac
//
LOCUS       DAEMHW010000228         5185 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEMHW010000228 DAEMHW010000000
VERSION     DAEMHW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.228"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            153..866
                     /gene="ispD"
                     /locus_tag="KPK67_003385"
     CDS             153..866
                     /gene="ispD"
                     /locus_tag="KPK67_003385"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBG2460303.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            863..1348
                     /locus_tag="KPK67_003386"
     CDS             863..1348
                     /locus_tag="KPK67_003386"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBG2460304.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    1443..1651
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1715..3430
                     /locus_tag="KPK67_003387"
     CDS             1715..3430
                     /locus_tag="KPK67_003387"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG2460305.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            3552..4997
                     /locus_tag="KPK67_003388"
     CDS             3552..4997
                     /locus_tag="KPK67_003388"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG2460306.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
BASE COUNT         2117 a          547 c         1039 g         1482 t
ORIGIN      
        1 atattgtaat aaatatatgt gtttttcatt agcaaataga cattaactat ttatacaact
       61 gaaaatttaa tatataatta atcagatggt taaaataaaa atattaaaag ttttaataaa
      121 tgatggttaa aaataaaata cgaggtgata ccatgtatag tgttataatt gttgcagcgg
      181 gaagcggaag gagaatgaac ttagatataa acaaacagtt cataaaatta agagaaaaag
      241 aaattattgc tcatacaatt caagtttttt atgaaaatat aaatatagat gaaatagtgg
      301 tatgtataaa aaaagaagaa gaagattttt ttaaagaaaa tataataaat aagtataact
      361 ttaaaaatat aaaaatagca tatggcggaa aagaaagaca ggactcaata tataatggtt
      421 taaaaaaatt agataaaaat tgtgatatag tacttatcca tgatggagca agaccatttg
      481 tcgaccatag aataatcaat gaatctataa aggtagctaa agaaaaaaag gctgtggttg
      541 taggtgttcc agtaagtgat actataaaga tagtgtcaga tggaacagta caagaaactc
      601 cagaaagaaa tttgctgtgg gcagcacaaa cccctcaaac ctttgaatat aatctcataa
      661 tagatgctta tgaacaagca tataagaaca attattatgg tactgatgat tcaatgttgg
      721 tagaaaatat aggtcaaagt gtcactatgg taatgggttc ttatgaaaac ataaaaataa
      781 ctagtccaga agatttaaat atagcagaac aaatattaaa tatggaaaaa agagatgaag
      841 ttaacagtag aaggagaatt atatgagaat aggattagga tatgatgtac acaaattaac
      901 agaggataga aaacttataa tcggtggtgt agagatacca cacgataaag gtttgttggg
      961 gcattctgat gctgatgtat tgatacatgc aataatggat tctattttag gtgctctagc
     1021 attaggagat atagggaagc atttcccaga tacagatgaa gaatataagg gagcagatag
     1081 tatgaaatta ttagaacatg tgtataatct aataacaagt aaaggatata aaattggtaa
     1141 tatagacagt actataattg ctcaaagccc taagatggct ccatatattg agagtatgag
     1201 aagtaatata tcaaaagttt taaacacaga cattgataat ataaacataa aggcaacaac
     1261 agaagaaggt cttggtttta caggagcaaa acagggaata gcttcacaaa gtatttgttt
     1321 attattgttg acaagtcaga ataattaata tattattagt aataagttta atggatatca
     1381 agtaataggc ctatatagaa tatctataat gtatgaattt aagtgaaatt aatataaata
     1441 taaaattagc tatgataaag aaatagtaaa atgatgaaat cttcacagag agaaaacggt
     1501 ggtgagagtt ttcaagagga agtctttgaa cctgtctttt agcttaaagg tataaaaatc
     1561 tttacggtag gaaccgttat attcccaaaa gagagctatt catatagcta actagagtgg
     1621 taccgcggaa attaaacctt tcgtctctat acttgtatag agatgagagg ttttttatat
     1681 tttcaggaag ttaatattca ggaggaatct attaatgaaa atgtcaaaaa tgtttatgcc
     1741 gacattaaaa gaaatcccag cagatgctga gattacaagc catcaattaa tggttaggtc
     1801 aggcatgata aaaaaaatga cttcaggtgt ttataaccaa ttaccaatgg ggttaagagt
     1861 gttcaaaaaa attgaacaaa taattagaga agaattaaat aaaaaagatt gtcaagaaat
     1921 actttgtgcc gccttacttc cttctgaatt atggaaagag tctggaaggt ggactgctat
     1981 gggtgaagaa atgtttagac taaaagacag aacagaaaga gagtactgtc ttgggccaac
     2041 acatgaagaa gcattcactg atataataag acaagaaatt acttcatata aacaattgcc
     2101 actgaacttg tatcaaatac aagtaaaata tagagacgag agaagaccaa gatttggtgt
     2161 tatgagaact aagactttta caatgaagga tgcatatagt tttgatgtag atgataaagg
     2221 tttagataag tcatatcaag atatgtttga tgcatatgta agtatatttg atagatgtgg
     2281 attggagaat agccctgtac aggcagattc aggagctata ggaggttcta catcagccga
     2341 atttatggtt aagtcagaag ttggtgaaga tgaagttgta ttttgtagtg gatgtgatta
     2401 tgcagccaat gtagaaagag cagaatcttg taatctagca tcacaaaaag aagaaatgaa
     2461 agaattagaa gaggttcata caccaggagc agctacaata aaagagttag aagaattttt
     2521 aaaaacttct ccagataagt ttgctaaaac tttagtatat gaggcagatg gaaaaacagt
     2581 tgtagtcgtt gtaagaggag atagagaagt taatgaaatc aaagtatcta atgccatagg
     2641 ttctgttata gaatttgctc ttgctacaga tgatgtagtg agaaaagtaa ctaatgcaga
     2701 agtaggattt gcaggtccaa taggtataaa tgctgactat gtatttatag ataaagaaat
     2761 tgttgaacaa agaaacatag ttgttggggc taataaaact gagtaccata taaaaaatgc
     2821 taactatgga agagattttg aaggaatagt tggagacttt agaaatgttc aagaaggcga
     2881 caagtgtata gtatgtggta agccacttga aatagctaga ggtgttgaag taggacatat
     2941 atttaaacta ggaactaagt actcagaatc tatgaatgca aacttcatag acaaagatgg
     3001 aaaatcaaag cctatagtaa tgggatgtta tggaataggt gttgaaagaa cagcagcagc
     3061 tataattgaa caacataatg atgagaaagg tataatatgg cctttgtcag tagctccata
     3121 ccatgttgtg atagttccag caaatatgaa aaatgaagaa caaatatcaa tagctgaaaa
     3181 tatatataat gatttgcaag ctatgggtgt agaagtttta ttagatgaca gagatgaaag
     3241 aataggtgtc aaattcaatg attcagaatt aataggaata cctatgagga ttacagtagg
     3301 taaaaatatt aatgaaggta aagtagaatt taaacttaga cataaagaag ataaagagat
     3361 aattgacata gaagaaataa atgaaaaagt aaaggcagag tttatcagga ataatgttag
     3421 gttaggataa taacttaaat aagatattgt atagataatc aaagttgtac tttatagaca
     3481 ccattttatc tatatataag gatataaaat ggtgtttttt tataaaacaa ataaatgagg
     3541 aggtaattaa aatggcaaaa aatgaaaaac aatttgttga agaaataaca aagatggaag
     3601 atgattttcc acaatggtac acagatgtaa taactaagac tgacttagtt gattatgcac
     3661 cagtaaaagg ttttatggtt gtaaagccat atggatatgc attatgggaa aagatgcaag
     3721 aatttatgga taaaaaattt aaagaaactg gacataaaaa ctgttatttt ccattattaa
     3781 taccagagag tttattaaac aaagaagcag aacacgttga aggttttgct ccagaagttg
     3841 catgggtaac acatggagga aataaaaaat tagaagaaag attatgtgta agacctactt
     3901 cagagactat aatatgtaca atgtatgcga aatggttaaa atcatataga gaattaccat
     3961 atctttataa tcaatggtgc tcagttgtta gatgggaaaa atcaactaga cctttcttaa
     4021 gaacatctga gtttttatgg caagaaggtc atacgttaca tgaaacagca gaagaagcac
     4081 aagaagaaac aatacaacaa ttagaagtat ataaagcgct atgtgaagaa ttacttgcaa
     4141 tgccagttgt agctggtcaa aaaagtgaaa gtgaaaaatt tgcaggtggt gaaagaacat
     4201 atactataga agctatgatg catgatggta aagcattaca atcaggtaca agtcacttcc
     4261 taggtcaaca ctttactaag gcttttgata ttacttttgc agatagagaa ggaaatttag
     4321 ctaatccata ccatacatct tggggagcat ctacaagact tataggtgga cttataatga
     4381 ctcatagtga taacagaggt ttagtattac caccaagagt agctccaatt caagttgtta
     4441 tagttcctat agctgctaaa aaaggaaatg taatggagac tgttgataaa atatatgctg
     4501 atttaaaagc taagggagta gctgtagaag ttgatgatag agataactat acaactggat
     4561 ggaaatttaa tgagtgggaa atgaaaggtg ttccagtaag agttgaaatt ggaccaaaag
     4621 atatagaaaa taatcaagct atggtattta gaagagatac tttagaaaag gattctatgc
     4681 ctttagaagg attagctgat gctatatgtg atttatttga tgttatacac aatgatatgt
     4741 ttgaaaaagc aagaaaacat agagaagaca atacatctat agttgaaaat atggatgagt
     4801 ttagaaaagc tttagaagaa aagccaggat tcataaagac tatgtggtgt ggagatgcag
     4861 aatgtgaagc taagattaag gaagaaacag gagcaactat aagatgctta ccttttgaac
     4921 aagaaaactt aggtcacaag tgtgtgtact gtggaaaaga agcagatagt atggtagtaa
     4981 tggcaaaagc ttactaaaat acatttaaat aaaaactggg gtagaaagta tttaatactt
     5041 tatcccagtt ttttgttttt tatgagtaat aaaatgtatt tgtaactatg tcataagata
     5101 agtcatagat acttatatta aaaagaatat aatagagaac tatttttaat aattacatat
     5161 taatgaaagc aaaatatttt tgtta
//
LOCUS       DAEMHW010000229         5152 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEMHW010000229 DAEMHW010000000
VERSION     DAEMHW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.229"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            140..1912
                     /locus_tag="KPK67_003389"
     CDS             140..1912
                     /locus_tag="KPK67_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460307.1"
                     /translation="MENVKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
     gene            1934..3355
                     /gene="ccpM"
                     /locus_tag="KPK67_003390"
     CDS             1934..3355
                     /gene="ccpM"
                     /locus_tag="KPK67_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HBG2460308.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVSKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            3384..4454
                     /locus_tag="KPK67_003391"
     CDS             3384..4454
                     /locus_tag="KPK67_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HBG2460309.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            4470..4721
                     /locus_tag="KPK67_003392"
     CDS             4470..4721
                     /locus_tag="KPK67_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HBG2460310.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            4772..4975
                     /locus_tag="KPK67_003393"
     CDS             4772..4975
                     /locus_tag="KPK67_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460311.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
BASE COUNT         2096 a          456 c          817 g         1783 t
ORIGIN      
        1 attttacaat tctattaaca attttatttt aaaggtttaa tttaaaattt aaatatgtat
       61 tatacataat aaaataatgt tatattatat taattaataa aatattttat ttcaatttta
      121 ttaagtatgg aggtgtattg tggaaaatgt taaatttcaa ttttttaaag gtgtaagaaa
      181 ttgttttaat atgatttatc tacatgataa aagatattta tttatctcta tgtttatggc
      241 aatttttcag gggattgttc catctatttc tttgttagta agtcaaaata tcattaataa
      301 tatgcaaaca ttttcgaaaa gtgtaaaaga aatattagtt ttgttaatta tgtatttaat
      361 cataaatata attccttcaa tttttcaaag tttttatgga tattatagaa taaagtttaa
      421 taaaaatttt gaaaaacata ttgaaacaat gatgttagag aaatctttaa ctttgagttt
      481 aaaagatttt gaggatgata atacttatga catgataaat agagctcaat ctcaaaaagg
      541 atttaatatt actatactat ctgaaaacct tatttcaata ataagagaat cagtatctac
      601 actttctctt attgttgtat tatcacaatt tcaactttgg ctaataatag ttgttattgt
      661 tattcctgta ttgaaatcat tatattccat taaattagga aaggaacaat atgatgttag
      721 tgtaaacaga acttggaaag aaagaaaggc ctggtacata gattatttgt tgtcaaaagg
      781 ttatgcttac aaagagatta tggtatttag aattggttct aaacttatta atcattataa
      841 aaaattaaaa gaggatatta tatctcaaga tatgaatata gctaaaaaga attttattgc
      901 tacgctatta tgtacagttt tagattcaat tgtatctgca ggaattatag gatttataat
      961 aattcaaggt attttaaaga agattttaat aggagatgcg actacatata tacaagcagt
     1021 tcaatctata aaggataatg ttgaatcaat attttcgtta tttagttcag taatacaaga
     1081 tcttttttat acagatttac tgtttaaatt tctagattat aactgtaaag aagactcaat
     1141 tgaacatata aaaatatttg aaaaaatagg ggatataaat aaaatagagt taataaatat
     1201 ttcatataaa tacaatcatt catcagagta ttctttaaaa aatattaatt taactttaaa
     1261 taaaaatgat cctattgcta tactaggaaa aaatggttca ggtaaaacga cacttttaaa
     1321 aatattatta ggtttttatg atgattatga aggtgacatc ttgataaatg gaaaaaattt
     1381 aaaggatatt gaaaaagatg aatacattag aaaagtaagt tgtatgtttc aagattatat
     1441 taaatatgag gctacattaa aagaaaatgt cgccatggga gatgtatata agagtaactt
     1501 ggaagaaaaa gttaaaagta aacttaaaga aactaaattg aaaccaagta tttatgagga
     1561 agatggatta gaaactattt taggaacctg gtttggtaag aagcaacttt ctatgggtga
     1621 atggcaaaaa gtagcaatat ctagaactct tatgaaagat gctgatatgt acatttttga
     1681 tgaaccagat tcatctttag attctgaagc gggaaatgaa ctcctttctt tatataaaaa
     1741 aacatttgaa aataagattg gaatatttat aacgcaccat atccaacatg caaaaaagat
     1801 aactaacaat attgtagttc ttgaaaatgg aaagttgtta gaaagtggaa gacatgatga
     1861 acttatggac aaaaagggtg tttattttaa tttattttgt aactgcaaat aaatattaaa
     1921 gggaggttaa ttaatgaatc aaaaaccatt tatacacaca tttaaagctg gaaaatcata
     1981 ttttatttat gatgttaata cggataagat tttaaaggta aatgctgctg tttataatta
     2041 tctaaataaa attgagcata atttagaaaa agaaagtgac tggaacatag aaatagaaga
     2101 cgaaattaat accttgatta attatggatt tctaaaaaat aaaagggtaa gtaaaacaat
     2161 gcaccctgaa actaagtata taaaatatca tatagaaaat aatctatcct ctattatttt
     2221 acaagtaacc caaaattgta atttacgttg tgactattgc atttattctg gttcatacag
     2281 caacagagtt catacaaata aaagaatgaa tatagatgta gcaaaaagaa gtattgacta
     2341 tttgattaaa aattctagtc aattaaatga agttagtgtt ggcttttatg gaggagaacc
     2401 cttacttgag tttgatttga ttaagcagat agtagaatat gtaaaagtag ttggagaagg
     2461 aaaggaaata tactttaatc taactacaaa tgcaacttta tttaatgagg aaataataga
     2521 attctttgta aaaaataata taacaactat gattagttta gatggaggaa aagaaattca
     2581 tgataaaagt agaaaatttg caaatacagg agaaggatct catgatgttg tattaaaaaa
     2641 tgtacgttac ataaaaaata aatatccaga atattataaa aagaatatta catttaatac
     2701 agtgataaat tcagaaaatg attacatttc tatagatgat tttatatcta aaaatgagac
     2761 tttaaaagat tcaaactttt tagcttccac tcttaataat aactattctg ataaagaaat
     2821 agtttcatca gatgaatata tattaggtaa gagttatgat agattcttga ctttactcaa
     2881 caaattaaaa tttataaaag atgagagtat gtcaccttta tcaagaatag aaagagcctt
     2941 attagatgat tttagatatg agaaaaatat tgaaggtaga ttagagttac cagatgtgtg
     3001 gcatcatggt ggaccatgta taccaggagc ttttagaata tttgtaacta cagatggaga
     3061 attttttcca tgtgagagag taagtgaaag ttctaaaatg ggtatgattg gaaatttaga
     3121 tagtggagta gatatagaaa aggctgaaaa acttttaaat ataggaaaat tatatgaaga
     3181 gcaatgttct gattgttggg cctatagtta ttgtgatata tgtatagcta agattgatga
     3241 tactaaatgt aatgaaaatg aaataaaacc acatgattgt gacagaatta gacattctac
     3301 agaaaataat atgaaagatt actgcgtttt gaaatctcta ggatatcaat attaaatttt
     3361 ctaatgaaag gaaagatggg attatggaaa aagtacttat atatccattt aattataatt
     3421 tttttactat tttaaatcat tgcaataaaa tttcagatat agaaattaat tcactggtta
     3481 gtccttctgg ctgggggtta tgcaataagg agtataatgt taataatatt attactaaag
     3541 ttacaaatga ttttcaaagt gaacttaaaa agaattcttt ggtatggttt gttgattcaa
     3601 aagaaaatct agacttagaa aaatttataa ttcctaaatt aaaagtagct ctaaaagaag
     3661 gtaaaaagat attatataca cgtgatgatc ttaataaaat atctaggtat atatctcatt
     3721 atgaattcaa aaagattaat gaatggaatt tagaaaaata tgaaataaga gatatagagg
     3781 gtatatcaca gataaacaca ccaataatat ttatttgtgg tctttatgga gggttagata
     3841 cttttgatgt aaatataagg ataggagaag aactaagaaa aagggggttt tcaatattac
     3901 aatttggccc taaaaaagag agctatttat ttaaaatgat tcctatacca gagtttatgt
     3961 ttgatgattc gataagtagt aagacaaaga ttattaattt taaccatttt ataaaagata
     4021 aagaggtaga ggcaaagcca gatttaataa ttatagaagt tccaggagaa ttattccctt
     4081 tctctagaaa aatggttggt aattttggaa ttagagcttt tgaaatttct aatgcagtaa
     4141 taaatgattg tggtatatta tgtattccat caagtatata tgatagagaa tattgtgaag
     4201 aacttaaata ttttattaaa ggaagacatg gtataaatat agactatttt agtatagaga
     4261 aaaaagttat agatacatat gagacagagg tgaagcaaaa attttcatat atgtcattag
     4321 atgaaaactt ttttgttgaa gatatagaag attatgaaaa aacatttttt atacatgata
     4381 ataaacaatt actagaatta gtggacaata tagtgaatca attaaaagag tatgctaaag
     4441 taatatctat ttagaattgg aggttaatta tgaattacac tgaaaaggaa ttactactag
     4501 agaatatatt ttttgatgtg actggaatta attttaataa aaataacaat ttaaaaaaag
     4561 aattattttt ttcaccgtct ttacatatac agccaagaga attactttta gtattttgcg
     4621 aggtaataga aaactttaat ataaaaattc cagaggaaaa acttttgaat aggcaattta
     4681 cgagttttga taatgttcta aaaataatca atatggtata ggttaataaa ggtataaagt
     4741 aagtgatctt atagaaaagg ggaggatatg aatgagtttt ggaaaaagaa tagaaatgaa
     4801 taatataggt gttgagacaa gaggtgtttg tactattatg tgtccttata cttgtaagat
     4861 gatggcatct tcatcttgta actgcaagaa taatgattat aatgcaagag gaggatcata
     4921 taatacatat tataataaat tgatacatgg ccaaagaact cctgatggaa tgtaaatatt
     4981 tcagtaataa gtatactatt aaattgattt agcatatttg attaaattta tctttcttac
     5041 tttatttcct tttattataa aaatatgcag ttgattataa ttaagagtgt gattttataa
     5101 taactgcata aatagtatct gttatataaa tcacctcttc aattattata gt
//
LOCUS       DAEMHW010000230         5119 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEMHW010000230 DAEMHW010000000
VERSION     DAEMHW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.230"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            103..1431
                     /locus_tag="KPK67_003394"
     CDS             103..1431
                     /locus_tag="KPK67_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBG2460312.1"
                     /translation="MEVNVKKINNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            1465..2784
                     /gene="der"
                     /locus_tag="KPK67_003395"
     CDS             1465..2784
                     /gene="der"
                     /locus_tag="KPK67_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBG2460313.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTLFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            2819..3433
                     /gene="plsY"
                     /locus_tag="KPK67_003396"
     CDS             2819..3433
                     /gene="plsY"
                     /locus_tag="KPK67_003396"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBG2460314.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            3464..4483
                     /locus_tag="KPK67_003397"
     CDS             3464..4483
                     /locus_tag="KPK67_003397"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG2460315.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(4640..>5119)
                     /locus_tag="KPK67_003398"
     CDS             complement(4640..>5119)
                     /locus_tag="KPK67_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2460316.1"
                     /translation="ISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDN
                     AQAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNL
                     VDTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
BASE COUNT         2024 a          551 c          975 g         1569 t
ORIGIN      
        1 gaaatgctta aaaatgctat cataaaatat aataaataaa taaattgcat attaaaatac
       61 attataataa gcattgatga atacaaaaga gtggtgaaaa tattggaagt aaatgtaaaa
      121 aaaataaata atattataag taaggtttat aaagatagta ttgcagaaga aatgggtata
      181 gaggtaggag atttacttat atctgtgaat gaacagccaa tacatgatat aatagagtat
      241 agattcttac taagtgatga atatttagat gtagaaatac aaaaaaaaga tggcgaagtc
      301 tacatatacg agatagaaaa agactatgat gaagatttgg gtgttgaatt tacaaatccg
      361 ataattgacc aagctaaaag ctgtagaaat aagtgtatgt tttgttttat agaccaattg
      421 ccagaaggta tgagagaaac tctttatttt aaagatgatg attcgagatt atctttttta
      481 caaggaaatt ttgtcacact tacaaatatg agtgaagaag atataaataa tattataaaa
      541 tatagaataa gtcctataaa tatatctgtg catacaacta atccagagtt aagacaaaaa
      601 atgataagca ataaatttgc aggaaaatta tatggtataa tgaagagatt ggcagatgct
      661 catatagaaa tgaactgtca aattgtttta tgtccaggta taaatgatgg aaaggaattg
      721 gatagaacaa taaaagaact agctcaactt tatccgtatg taaacagtgt tgcgatagtt
      781 ccagttggga ttacaaagca tagagaaaat ctagtagaat taaatatatt taatgataag
      841 agcgcaagta aaactataga gcaaatacat caaatacaac agaaatatct tgaaaaatta
      901 ggtactcgat ttgcattttt atcagatgaa ttttatatac tatctaatag tgaattgcct
      961 ggatatgaag aatatgaagg ttttttacag tttgaagatg gtgtaggtat gatacgtaag
     1021 ttaaaaacag agattgaaga gtatctaaat atattgcctg agaagattct aaaaagagag
     1081 aagaaagtat caatagctac tggtcattct gcatatgaat ttatacagag tatggcagat
     1141 gctatgatgg acaagtttaa aaatcttcaa gtaaatgtat atgaaatcaa aaacaagttt
     1201 tttggagaga caataactgt atcaggtctt ttaacagcta aggatttaaa agaacaactt
     1261 gaagataaag aattaggaga agctctttat ataacaagaa gtatgctaaa agcagatgaa
     1321 gaaattttct tagataatat tgaattaaat caattagaag aacttatgag aataaaaatt
     1381 atcccttgtt tgaatgaagg aaaagatttt gtggataaaa ttttaaaata gaaaacaaaa
     1441 gatggaagga gaaataaaat gagtataagt aggccagttg tagctgtagt tgggagacca
     1501 aacgttggta agtctacaat atttaataaa tttgcaggaa aaaggatatc aatagttgaa
     1561 aatacacctg gagttacaag agatagaata tttgctgaag tagaatggtt agataaatat
     1621 tttacattag tggatacagg aggaattgaa cctgatagcg aagatataat attatctcaa
     1681 atgagaaatc aagccatgct tgcaatggac atgtcacatg taatactttt tatagttgat
     1741 ggtaaagctg gaataacagc tgctgataaa gaaattgcac aattgcttag aaagacaaaa
     1801 aaaccagtaa tcttagtggt taataagatt gatagtcaaa gtcaatttga taatatatat
     1861 gatttttatg agttgggatt tggaacgctt tttgcagttt caggagccaa tagtatggga
     1921 tttggagatt tattagatga gatagttgaa aattttccag caggattaga tacagagtat
     1981 gaagaagata taataagagt agctataaca ggtaaaccaa atgcaggaaa aagttctata
     2041 ctcaataaga tacttggaga agaaagagta attgtaagtc caattgcagg aactactaga
     2101 gacgcaattg atacttattt tgaaaaaaat ggacagaaat ttcttctaat agatacagca
     2161 gggcttagaa gaaaaagtaa aatatatgag acaatagaaa aatacagtgt aataagagct
     2221 atgagtgctg tagatagagc agatgtagta ttgatagtaa tagatgcctt agaaggtgta
     2281 acagaacaag atacaaaggt tgcaggtata gcacatgatg aaggaaaagg atgcatattt
     2341 gtaatcaata aatgggattt aatagagaaa gataataaga ctatgagtaa ttacacaaaa
     2401 gatataaaag aaaaatttcc atttatgatg tatgctccaa tcgtatttgt ttctgcaaaa
     2461 actaatcaaa gaatgaacaa gatactagat acagttgaat atgtgtcaaa tgaacattct
     2521 aagagaatat caacttcagc actgaatgaa gtaattggag aagctgtaat gttaaatcaa
     2581 ccaccatctg acaaaggtag aagattaaaa atttactatg ggactcaaac tgacattaga
     2641 cctcctaaaa ttacattgtt tataaatgat aaggatttaa ctcacttctc atatcaaaga
     2701 taccttgaga ataagataag agaaaatttt ggatttgaag gaacttccat aaagtttgag
     2761 tatagacaaa aaaataaaaa ataaatatct taaaatataa ctaaaggggg gaaattcagt
     2821 ggagatattt agttatataa ttattgctgt agttgcatat cttttaggaa atatatctac
     2881 ctcatacata gttgcaaagc gaattgctgg ggttgatata aggactcaag gttctggtaa
     2941 tgcaggttcg acaaatgtac ttaggacact tggaaaaaga gcaggagcaa tgacattttt
     3001 aggtgatgtt cttaaaggtg taatggctgt tttaatatca gaatttgcag caagattggt
     3061 tggaatagat actcttttag ctggatatct ggcagttata tgtgttgtag caggtcataa
     3121 ttggccagcg gttttaggat ttagaggagg caaaggtgtt gcaacttcac taggggcaat
     3181 gctagctgta aatccagtaa taacactaat gtgtctagca gtatttatat tggtggtagc
     3241 tataacaaag tatgtttcat tgggttctgt tgtaggaata ggatgttcac caatatttat
     3301 gattatggta aaaaataaag ctggtttaat agtagctctg ttcttaactg catcagtaat
     3361 atataatcac agagcaaata taaagcgatt attaaatggg acagagagaa aaattggtca
     3421 aaagaaagaa tagtaaatat attatactca ggtggtgtta aacatggaga aagtatgtgt
     3481 attaggtact ggaagctggg gaagtgcatt agctttaggg cttgcaaaaa aaggtaatga
     3541 tgtgagcatg tggactcgaa aagaggaaca agctaaaaaa ataaacagaa ctaaagaaaa
     3601 tacagactat ttgccaggag tattatttcc aaataatata actataagta ctgatataga
     3661 aaaaactata aaagattgta aggtaatagt tttagcagtt ccatcacagg ctgtaagaag
     3721 tacttgccaa aaaataaaac catttataaa agaaggacaa gttattgtta atgtagctaa
     3781 aggtcttgaa aaagggactg ggcttagatt atctcaagtc tgtgaagaag aattaccaca
     3841 aaatccatat gtaatactat ctgggccatc tcatgctgaa gaagtagcaa gggatattcc
     3901 tactaccgta gtagttgctt ctaaagattt aaaaatagca cagatgatac aagatttatt
     3961 tatgagccct aaacttagag tttatacgaa tccagacata gttggagtag aacttggagg
     4021 agcattaaaa aatattatag catttggagc tgggatatgt gacggattag ggtatggtga
     4081 taatgcaaaa gctgccctta tgacaagagg tattagtgaa atgagcagac ttggaattgc
     4141 tatgggagct aatatgtcta cctttgcagg tctttcaggt attggagatt taatagttac
     4201 ttgtacaagt atgcatagta gaaatagaag agctggtata ctcataggta aaggtatgag
     4261 tttagaagat actttaaaag aagtaaagat ggttgtagaa ggcataactg ctactgaagt
     4321 agcacatgat gttgctgaaa aattagatat agatatgcct ataactaatg ctatatattc
     4381 tgtaataaag aaaggttcaa atcctaaaga agttggaata gaactaatga tgagaagtaa
     4441 aaagcatgaa atggaagaag tagttttagg tgatgatata taaactattg tataataaat
     4501 aataaatttg ttttaggggt gtctcaaaat gaacttttta gtttacgagg cactcttttt
     4561 tatatgaaat taaatatatt tttattattt caacaataaa aaaggcttat ctctattttg
     4621 agacagccat attttaattt tatttagcat gttttattat gtattcaata gaagaaatca
     4681 ttttcatagt taattcatca tcatcagaat tttttcctgt gttctttttt aagaataata
     4741 gattttgata aaaagaaata attatatcat tgattatgtc taaatcttta gctggtatat
     4801 atccagtatc tacaagattg tttaatagta tcttatttct ttcctttata ctttctcctg
     4861 taatcattaa agatatgtca ctatcatgaa tacctaaatc ttctggaaac atatcataat
     4921 attgttttgt tatatagcta agttcatcac tagacaagtt aaaaaatacc tggtaaaaag
     4981 cttgagcatt atcaaaagaa tgcttacaaa ataatttcca cataattata tatttcttat
     5041 attcatcatt gacattttct atacatcttg aaatattcaa gttataatct ttaaaatatt
     5101 ttatagatgc aaatgatat
//
LOCUS       DAEMHW010000231         5087 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEMHW010000231 DAEMHW010000000
VERSION     DAEMHW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.231"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(112..1299)
                     /locus_tag="KPK67_003399"
     CDS             complement(112..1299)
                     /locus_tag="KPK67_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2460317.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            1572..2396
                     /locus_tag="KPK67_003400"
     CDS             1572..2396
                     /locus_tag="KPK67_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2460318.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHREEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            2525..3790
                     /gene="gluD"
                     /locus_tag="KPK67_003401"
     CDS             2525..3790
                     /gene="gluD"
                     /locus_tag="KPK67_003401"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBG2460319.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            4049..4639
                     /locus_tag="KPK67_003402"
     CDS             4049..4639
                     /locus_tag="KPK67_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG2460320.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFIYIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADAPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
BASE COUNT         1955 a          567 c          832 g         1733 t
ORIGIN      
        1 acagtaaatc aataaataga gttattctaa aataagaaaa atgacaaatc ttatttagaa
       61 atagctctat tttttgtaat taaatttaaa ttatattcaa tttgtaaaat attattttga
      121 acaattatac atatactctc tagttagagg taaatcattt tttatacctt tagtgaaaag
      181 tatttgatgt atatctataa taccagtgtt gaaagcagca gcacaagaac aaaggtataa
      241 atcccacatt cttataaact tatcatcaaa catttcttta atctttggaa gattctcaga
      301 gaaatttttt tgccagtata gtaaggtttt tacataatgt aatcttaaac tttctacgtc
      361 tatagtatga tatttgtaat cactacaaat atgtatcatt tctcttaggc taggaataac
      421 tcctcctggg aaaatatatt tttttatcca agggtctcca taagattcct gcaatccact
      481 tatatagtga agtaaaaaca atccaccatc ttttaaaaca gcatttacgt ttttaatgaa
      541 aagttcataa ttttctctac caacatgttc aagcatccca acgcttacaa ctctatcaaa
      601 cataaggtct gattttttta attctctata gtccataagt cttacctcaa gatagtcttg
      661 aaggttctct tctttaattc ttgcgttaaa ttccttaaat tgctcttcgc taagtgtaat
      721 tccaacgccg tgaattttat atttttttgc agcttctatt agtaaaaatc cccaaccaca
      781 accaatatcg agaaggctca taccttcctc taaatttagt ttagttaata tgtggtgaac
      841 tttattgact tgagcatcat aaagagtatc attatcattt ctaaagtatg cacaagaata
      901 actaagtgtc ttatctaacc ataagctata aaaatcgttg ccaatgtcat agtgggcatg
      961 tatttctttc ttttgatgtc tcattgaatt tgatgagtgc aatagatgtt ttacagctga
     1021 attatcaata ttgaatttgt ctatttgttt taaaagtaga tttaaagtta agaataaatc
     1081 tccctcaact tcaatgtctc tatttatgta ggcttctcca agtgctaaag atgtactttt
     1141 taatagttca gacttgctaa tatcattatt tactataact ttgaactgag gggagccctc
     1201 tccaattaga aattcttcct catctttaag cctaactaga aatggtgttt caccaaattt
     1261 ctctaaatag tcttttaaaa aattttttgt aatattcata ttaccagctc cttctaatgt
     1321 aaatccagta agtttccctg cttaaaatat attatagtcc tttttttaaa aaagtaaaat
     1381 tttttcaata aattttttat ttaaaggaaa tttatttttt ataaagaaat ttcatgttgt
     1441 agtatatttt ttgcttataa attttggtac tggatataat attaaactgt taatgatata
     1501 ataaatttaa tctataaagt aaataaaatt ttgtaataaa tagttaaagc tattttataa
     1561 ggagatatat gatggaagta tatgctttgg ttgggtcaag tggcacaggg aagagcttta
     1621 aggctttaga gttcgcatat gaaaatgata tagaatatat aatcgatgat gggattttaa
     1681 tatacaaaaa caaggtttta gctgggatat cggcaaaaca agcaaatact actatagaag
     1741 cagttaaaag ggctattttt tataatttgg agcatagaga agaagttaga gaagcaatta
     1801 aaaaagaaaa tataagaaaa attcttatta ttgggacatc aaaaaaaatg gttaatcaaa
     1861 ttgtagagag attgtcaatt ggaagggttt gcaagtttat aaatatacaa gatataagta
     1921 ctaaatatga aatagaaata gcaaaacaag ctagaaaaga aggaaatcat gtaattcctg
     1981 ttccagcagt tgagataaaa agtatggcaa gtggtcttag tataaattct ttgaagagac
     2041 tttttagaaa aggaaataat aggaatatgg tagttttaga aaaaacaatt ataagaccaa
     2101 cttttagtta tataggaaag ttttatatta gtgctgatgt aattaaacag attattgaat
     2161 atgaagtata caactttgga agcattgata gaataaataa aatcaatata gagaattgca
     2221 ataattttat gaatattttt gtaagcataa atatcaatga tttagaaact ataaaaaaaa
     2281 ttgaagacat acaacagaca ataaaaaagt ctattgagaa aatgacttta gttaatgtac
     2341 aaaaaataga tattaacatt cataaaatta aaaaaataaa cttcctccaa tgttaaaaaa
     2401 taaactgaga aaatgatata ctaatttgca ataaagtgtg ctataattaa actgtaaatt
     2461 taggaaatgg ttttttctaa atggagtata cataattata acgtatttat aagggggctt
     2521 tcaaatgtca ggaaaagatg taaatgtctt cgagatggcg caatctcaag taaaaaatgc
     2581 atgtgataaa ttaggtatgg aaccagcagt ttatgaatta ttaaaagaac ctatgagagt
     2641 tatagaggtt tcaattccag taaaaatgga tgatggttct ataaaaactt ttaaaggatt
     2701 tagatcacaa cataatgatg cagtagggcc aacaaaaggt ggaataagat tccatcaaaa
     2761 tgtttcaaga gacgaagtaa aagctttatc tatatggatg actttcaagt gttctgtaac
     2821 aggtatacca tatggtggag gtaaaggtgg aataatagta gatccatcaa ctttatctca
     2881 aggtgaatta gaaagattaa gtagaggata catagatgga atatataaat taataggtga
     2941 gaaagttgac gttcctgcac cagacgtaaa tactaatggt caaataatgt cttggatggt
     3001 tgatgagtac aataaattaa ctggacaaag ttctataggt gttataactg gtaagccagt
     3061 tgaatttggt ggttctttag gaagaacagc tgctactgga tttggtgttg ctgttactgc
     3121 aagagaagct gctgctaaat taggaataga tatgaaaaaa gctaaaatag ctgttcaagg
     3181 tataggaaac gttggttctt atacagttct taactgtgaa aaacttggtg gtactgttgt
     3241 agctatggct gaatggtgta aatcagaagg ttcttatgct atatacaatg aaaatggttt
     3301 agatggtcaa gctatgttag attatatgaa agaacatggt aacttattaa acttcccagg
     3361 agctaagaga atatctttag aagagttctg ggcttcagat gttgatatag taataccagc
     3421 tgcattagaa aactctataa ctaaagaagt tgctgaatct ataaaagcta aattagtttg
     3481 tgaggctgct aatggaccaa ctactccaga ggctgatgaa gtatttgctg aaagaggaat
     3541 agttcttact ccagatatat taactaatgc tggtggagtt acagtttctt actttgagtg
     3601 ggtacaaaac ttatatggat actactggtc agaagaagaa gtagagcaaa aagaagaaat
     3661 agctatggtt aaagcatttg aatctatttg gaaaatcaaa gaagagtaca atgttacaat
     3721 gagagaagct gcttacatgc attcaattaa gaaagttgct gaagctatga aattaagagg
     3781 atggtactaa gatttactct taataaataa taggatataa ttatttaaga tataaagggg
     3841 ctgtacatag tacagccctt aaattatgtg gcttacaatg aagtaagcta tttttgtttt
     3901 aacttataaa taacttgata aattatgtaa tttagaatgt ataaataaga tgggtgaaaa
     3961 ttaatggttg atttaatttt gaatggaatt tttaccacca catgattaca aattagaaga
     4021 gttagattag atttttagac atgaacttat gcattttaaa agcaaagacc ttaatttaaa
     4081 gtatctagta ttatttttaa aaatagtata ttggtttaat ccatttatat atataatgga
     4141 aaaatatatg gatttggatt gtgaacttta ttgtgatgag agagttttaa aaaattgtac
     4201 tgtacaacaa aagcaagaat atgctctaac tattgtaaat tcaatgaaaa gaagtacaaa
     4261 tttttcaaat aaatttgtag ctggattaaa taaacaatct gatattagaa aaagagtaat
     4321 aaacatgttc agtgaaaaac ataaaaatgg tatcttaagg gctttgatta taagcttgtt
     4381 ttcttctatt acatttttta aattcgattc aactagtatt attgaagaga atgatttctt
     4441 agagaggtca agatatacgt ttgaaggaag gcaagaagac atgtttgtaa caatagattt
     4501 tatatatgca gatgcaccta taaaacttag aaaagagcat gaagaaaact gtagagtcaa
     4561 tggaatagta ccaaaagatt cagattcaat agaaattatt cctagatttt ataaagattg
     4621 tatggattta aaaaaataag ttagacttaa tattgatgtt gatattatga attttttata
     4681 tttaagcaat tgaaaatttt attatagatt caattttata tgtgtttaag tatgaaattt
     4741 atagatagtt gaaataatta aatatgattg atgcatagga atcattacta agctataatt
     4801 aattataggg gagtttaaat aaattatgag ttacacaatt ataaaatgat aaaaatagtt
     4861 atctgttaca aaaaatgtat ctttttaatt aataaagata atatgaaaac ttaaaaggca
     4921 aataaactta aatataaaat actcttaaat aagataggag aaaaatgtat gtctaacttt
     4981 attctaaatg ggatttttag aacagttata gtaagtagtc taagcattct tctgatattg
     5041 attttcaaga aaaatgtatt taaaagattt agcaaaaaat ttaatta
//
LOCUS       DAEMHW010000232         5083 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEMHW010000232 DAEMHW010000000
VERSION     DAEMHW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.232"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            224..2128
                     /locus_tag="KPK67_003403"
     CDS             224..2128
                     /locus_tag="KPK67_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2460321.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEEKGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKIA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
     gene            2488..3267
                     /locus_tag="KPK67_003404"
     CDS             2488..3267
                     /locus_tag="KPK67_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG2460322.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(3577..4398)
                     /locus_tag="KPK67_003405"
     CDS             complement(3577..4398)
                     /locus_tag="KPK67_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2460323.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
BASE COUNT         2015 a          567 c          806 g         1695 t
ORIGIN      
        1 tttataattt gaacatgtta gaataagtct tatttgaaaa ttttagtgta ttagagtaag
       61 tattatttta ccagtaaaaa atattgagaa ctatatgtag atttgcttaa aaaggaattt
      121 acatatagtt cttttcgtat aataaatagt acaatcttat atggtaaaga taaagtgtga
      181 tattaaattt tattatcatc caaaatctaa ggaggagtct cttatgaaaa taaaatttat
      241 tatagtagca tcaataatat ttttaatagt attacttgtg gggtgtcaaa gtactaaaga
      301 cacagaaatc aatgtactcg ccacaagtga tttacattca attattccag ataatttaat
      361 ttcctatgta gaagaggaga gaaaatatga tgagaatcta cttatggtag atgcaggaga
      421 tttttttgac atgcaaagtg gtgagatgaa taagtggttt actggtcaaa aattagtaag
      481 atttaaagat gggataccag agtacaaaaa aatagctgaa ccacgtgaag gagaagttcc
      541 attggctaaa aaaatggcaa agctgaagta tgatgcagtt acatttggga atcatgaatt
      601 tgtatctaat gacaagcaga gccttgataa attggtttct gattttaaaa ataacaatat
      661 accattagta tcagcaaacg tatatgaaca atctggagaa aactacgtaa agccatatgt
      721 aatgaaaaat ataaatactg acaagggaac tgtaaaggtt ggtatacttg gtctgactat
      781 aaaagaagta ggggaacgtt caaggtttaa aagttttgaa caagataaaa aggctagaga
      841 acttggggaa caacctcaat acaaagggaa attatatgca aatgatttag tagaagatgc
      901 aaataagtgg gtaaaggtta tggaaaaaaa agaaaagcca gatataatag ttgccattgt
      961 acactcaggt gaagagccaa aaaatcctaa aaatccagga aatagaataa aagaactagc
     1021 aacaactgta gagggaatag atgctattgt agcaggtcat acacatgaaa aaatagaaca
     1081 gcatacatat aaaaataatt ctggtgaaga agtaatagta acacagcctg gagctcatgg
     1141 taatagtata tcaaagatta attttaagtt ggataagaag aatgataagt ggtttataaa
     1201 agacaaatat agtgaattaa aggtttttgg aaaggaagta aatataatta caacttctgg
     1261 tctgaatcca aaattttcag atgaaatggc agttaatttg tttaatgaaa gagatagaga
     1321 tgttcaacct atctttatag atactggaga ttttttaaat actaatacaa aagaaatgac
     1381 taagtgggct aaggagtggc aatacaatga aaataaaggc atctataaaa aaataaatga
     1441 atggccaatt gtatatcttg gttatgatgc tgtagtactt ggtagccatg aatttacaga
     1501 ggataaagag acttttgagg aaaaaaaatc tacattggat tatattatag aaaactttga
     1561 agaaatgaag ataccagtat tatcagctaa tatatatgat gaatctggag aaaattatgt
     1621 aaaaccttat ataattcata atgtagaaac tagggaagga aatataaagg ttggagtatt
     1681 aggtcttact actactgata aggatgaaga agacttaagt agctcaagct taagcaaaga
     1741 ctcaaagact tcgaaaattc ctgaagaaaa aggaaagtta tatactaaca atctagtaaa
     1801 agatgcaagc gaatgggtaa aagttatgca aaaggaaagt cctgatgtga ttgttgcagt
     1861 tgtacattca gataataata gtactctaaa aaatccaagt agtgaagtca aaaaaattgc
     1921 aatgtcagta gatggaatag atgctattgt tgtaggtcat acgaaaaata aggtagaaga
     1981 acatatttac aaaaataagt ctggggatga ggttatagta acacaatctg gagaagattg
     2041 ttattcaaaa ataagtcttg aactaagaaa agaaaatggc aagtggaata tcataaacaa
     2101 atatagtaaa gcaataaaaa tgaagtgaat ttattgatat ataaaccatt attaacactg
     2161 atttttataa tataatcaat actgattcta taatataatt aacactactt tttaaatata
     2221 atcaatatcg attctataat attaacacta ctttttataa tataatcaat actgattcta
     2281 taatataatt aacactactt tttaaatata atcaataccg attctataat attaacacta
     2341 ttttttatgc tatataacaa ttattgaaaa tgatataaat ataatatctc aaataatatg
     2401 aatataacat ctgaatagag ggtgttttat ttgtaaaaaa taagtttaca gtttgagcgt
     2461 ataaacttat ggtaaaggag aataattatg agttataaga agattgcaat tataacgggt
     2521 gctaatagtg gttttggtaa agagtttcta aagttattaa taaatgaaga agaaataaca
     2581 gaaatatggg cagttgcaag aaacaaggcg agattaaatc aattagtaga tgaatttgga
     2641 agtaaggtaa aaattttttc aaaagattta tcaaaaatag aagaagtaaa agaaattggt
     2701 acatttttaa gtaaagaaaa tgtatgtata aaatacctta taaacaatgc tggttttgca
     2761 aaattctgtt catacaatga tttaagtata gatgaatcta ttaatatgat tgatttaaat
     2821 attagtgctg ttgtagcatt gggacttata tgtattccat atatgcaaaa gggaagccat
     2881 atcatgaata tttcttcaca agcatcattc cagccattac catatcaaaa tatttatagt
     2941 tcaactaaag cgtttataag aaattataca agagcattaa atgttgaact taagagtaaa
     3001 gggataaatg caattgctgt atgtccaggt tggatgagta caaatctatt taagagaggt
     3061 atagttgtag ctgaaaaagg gacaaaaaat ttcccaggca tagtgtctcc agacgttgtt
     3121 gccaaaaaag cattgaagga tgcaaagaaa aataaggata tttctgtgta tggtataaat
     3181 acaaaatcca gtcatttatt agcaaaacta ttgccacaaa aaatgatgat gaaggtttgg
     3241 ttaatgcaac aacatattaa ggaataagag tttcggtaat taagtatcag ttggagattc
     3301 aatctgttat atattatgag aagttttgtt gagcaaataa taattgtgac aaaacggctt
     3361 atctagtgac gataagccgt ctataatgta tataaaatat atttttgatt ttactttatt
     3421 tagatattga agtattaaaa tatgtaggac ttgaaatttt ataaagataa atactatgat
     3481 attttgtaaa aactgagata tgttgatatt acatagttta actatactgt taaattgaag
     3541 ttttcttatt ttgcattatg cttattactt aatgatttag actataattt ccataccatc
     3601 atatgcaaaa taaatgttat caagtttctt ttctaattca ctgtaataac catatgattt
     3661 tccccacatt tcgtcaatat gtgtaatgat aatcttttta attctataat agtttttaat
     3721 ttcaacaatc tcatctaaag taaatatttc atctttaaat ggtgtgtctt taagttttgt
     3781 accatcattt aatataccat catcagaaac tagacttatt attaaaatat cagcatcaaa
     3841 gtacatatca ctatgagtaa atggcttggg attacagcag gcatatatga cttttttttc
     3901 attctctttg ataacataga aggttatatt cctgtctggg ttattgttaa tcaaatcaat
     3961 atcaaaatta tttatcttta catgcgaagt ttcattaata gaaatgcaat ttataacatc
     4021 accataatat ttaagacaat cgagattcgt tcggtttata tcttcaatta cttcaggaag
     4081 agcataaaag ccaattggat tattttctcc tgtgataaag tcacatccta ttttttcgac
     4141 aattctaata cccttaacat ggtctgggtc atggtgagaa attgaaatat gttttacttc
     4201 cgatattttt tgtctgttac aagctactgc aatgtcttca ggtgtgtcta tcagcatttt
     4261 tatatcatca atatataaac ttggccctaa tcgttcatat cttccgcctt tttgtctggc
     4321 ttcttcacat attttgcatt tgcaaaatga acttggaata attgacatac ctccactacc
     4381 aacaattttt aatttcaaag ttattacacc accttcttaa aatattagat tatataaata
     4441 atatacaagc acaactgatt aacaaaatta tgagttaata aattatatca catgcaaata
     4501 tgaaagaata gtatgaaatt tttataaaca tatattaaat gtttctcaat gaaattgact
     4561 ttgtatatga actgaatgac ttattcaata tggataagac atttttcttg taataagata
     4621 tgattgtcct ataaggttta tgtaattttg atataatttg atataatata ttgattaaca
     4681 acatacatgt tattggtgag catatttcta tagaatggaa tatataattt tttatattta
     4741 tcattatatc attgtctaaa gaaagttaaa aagagtagag cacaaaggaa ttatttgtac
     4801 tttactcttt tattgttttg ttaaaaaaac tactttatat tagtgattaa aatatattta
     4861 actagagtat aaggatagta tataatctta taattgattt atctgacaaa ttaagataat
     4921 atcctaatca aattctagtc atttaaacat aaaatcataa ttgatttatc tgacaaattg
     4981 agataatata ctaattaaat tgtaggtatt taaacataac attataaata atttatctga
     5041 caaattaaga taatatccta atcaaattct agtcatttaa aca
//
LOCUS       DAEMHW010000233         5075 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEMHW010000233 DAEMHW010000000
VERSION     DAEMHW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.233"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            263..3361
                     /locus_tag="KPK67_003406"
     CDS             263..3361
                     /locus_tag="KPK67_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG2460324.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCGQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            3484..4770
                     /locus_tag="KPK67_003407"
     CDS             3484..4770
                     /locus_tag="KPK67_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2460325.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNYYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAISENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
BASE COUNT         2069 a          459 c          889 g         1658 t
ORIGIN      
        1 attgaggtgg agatttgtca tttatatgag aattaattgt aaatacataa taagatagtt
       61 aattataaat atattaaatt aagtattaca aaactctatt gtacgattga cacaataata
      121 attctattta ctaaaaaata gaacctcatt aaggtactat tttaaaacat aaagaagtat
      181 aatatcattg ccaaatatat agcaaaattt taaaagcttt aaaaattcaa tctggaaaat
      241 gaataaaagg agatatatat aaatggatgt aataaattta atagatgagt tggatgaaat
      301 tgtatatgtt tcttctttag atacatatga acttttatat attaatgaaa ttgggaaaaa
      361 ggcaatggga cttaatgata tatcccattt aaaatgttat aaagcattac aggggaaaga
      421 ttctccctgc gaattttgta caaactctat tttaaaagaa gatgaatatt acatatggga
      481 aaatacgaat actcgaacaa atagacattt tatattaaag gacaagttaa ttaaatggga
      541 gggcaaacat gcacgattag aaatagctct tgatattaca gaaaaggaag atataagtaa
      601 gtcaatagca gaaaaattag agataaagga gttattagtt ggatgtatta aacatcttat
      661 aacagaatct aatttttctg atgctgtaaa tcttgtatta accaatattg gtaaatttca
      721 taaagctgat agagcatatg tttttgaagt atcagaagat agaaaaaaag taactaatac
      781 atatgaatgg tgcaatgaag gtgttgatga acaaaaacag gcactaacaa atcttgatat
      841 taatgaatta aagaattggt ttttattatt cgaacaaaaa ggttttgtaa taattgatga
      901 tatagaaaat ataaaagaag aacagccaat agaatatgaa attttaaaac ctcaaaaaat
      961 tgaaagttta atgacatctt cactaaaaca agatgataaa gtagtagggt ttattggagt
     1021 tgataatcca cataaagctg ttggagatac ctctcttttg acatccctat cttatttttt
     1081 attaactgag atgaaaaaaa gaaaagtaga aaataaatta aattatatga gctactatga
     1141 cttcttgact ggtgcatata atagaaataa atatattaag tatatagata attttcaaaa
     1201 aggaaaagtt aaatccatag gagttgtatt tgtagatata aatgggttaa aagatattaa
     1261 tgataacttt ggacatgcta atggcgattc tgctattgtg tcagcatgtg taataataaa
     1321 aaaatacttt cattcttata atgtttaccg tattggagga gatgagtttg ttgtattatg
     1381 tgaagatata tcaaaacaac tatttgaaga attagtgagt tggttaagtg aagaatttaa
     1441 ggatgagatg atatatagtg tagcagtggg cagtatttgg agtgacgata atattgatat
     1501 acataagcta attaaagttg ctgatgagag aatgtaccaa gataagaaga ggtattataa
     1561 taagaacaaa cttagaaata atgaaatgaa agtaattagt gacttagctg attcgacgaa
     1621 taatatttta tttaaacata aagagttagt tgataaggtt tattttgatg atttaatgat
     1681 aaaagggtat tctaaggctt atgaaaaatt agcttataat acatatgata taattttatc
     1741 tatttgcatt gatacgaatt taattactcc aatttatcaa tcagagagca atatttttag
     1801 ttttcctata aaggaaaatc ttgaaaaaac tttttcatac ataaaaaaac atttaattca
     1861 tcctgatgat ataatattat ttaatgataa ccaccttcta aaatctcttg atagtcttaa
     1921 taatagcaag caagacgatt tagaactaaa ttttgagtat agacgtttag gaatagataa
     1981 aaagtactat tgggcattaa tttatatgtt aaagattagt agtaaaaata agttaggtga
     2041 gaaacaaaca catattttag ttgctataaa aaatatagat tatcttatgc aactacataa
     2101 aaaccaaaaa gatacattaa caggactata caattatgaa agtttttgtg gacaaataag
     2161 tttaatgctt aaaagatatc ctgacaagcg ttatgcaatt ataatgatgg atatagataa
     2221 gttcaaggta attaatgaca tttatggagt tagaggagga gataaagctt taatatatgt
     2281 tgcagagttg attcaaaaaa acattggttc taatggtata tgttgtcgta tgtatgcaga
     2341 tgtattttgc attttttatg agagttactc tgatagagat attcataagg taatttataa
     2401 gattaaaaat agcttaaata gaaaaaaatt tgaatatatg ttagtaccat gttttggggt
     2461 ttatagaatt ttggataatc atattccaat tacaaatgcg tgtgaaatgg caggatatgc
     2521 tcacaaaaaa gtaaagaaac aaagtatcaa taactatata ttttatgatg atactataag
     2581 aaatgatgca cttgaagaaa aacagatgga aagggaaatg gaatacgcac ttgaaaatgg
     2641 gcaattccat gtatatttac aacctaagta taatcttaat gcaaataaaa ttgttggtgc
     2701 agaagcattg gttagatggg ttcatccaga aaaaggtata atacctccaa ttaaatttat
     2761 ttcactattt gagaagaatg ggtttattat cagattagat atgtatgtat gggaacaagt
     2821 atgttctttg attagaaaat ggatagattt aggtgaagag ccagttccta tctctgtaaa
     2881 tgtatcaaga ttacatttgc ataatccaca ttttaaagaa attgttttaa gtttaataaa
     2941 taaatatagt ataccaaaat cttttttaga attagagctt acagaaagtt tatttattaa
     3001 aaatataaaa ttatttacta agataatagc cgaccttaga atggatggta ttgttttaaa
     3061 tatggatgac tttggttcag catattcttc attaaatatg ttaaaaaata ttaatattga
     3121 tacattaaag atagattgtg gtttctttaa tgaggaagga atcacagaca gagaaaaaat
     3181 agttttaaag cacactatag ccatggcaaa agatttagat atggatgtag tggcagaagg
     3241 tgtagagaca aaagaacaag ctgaattttt agtgagttta gattgtgtgg taattcaagg
     3301 ttattattat tgtaagccta ttccaagtgc tgattttgag aagttacttt atcaaaaata
     3361 agctaattat tccattctaa atgcaggtag gatggagtag ttcaaaataa ttaaatagaa
     3421 gtagaaaata gtgaggatat aatttttaaa aagtataata tttatggatt aggagcgaag
     3481 tttatgatag tgggtattat aggtccttca gactctgggt ttaagataaa ggatgattta
     3541 aaacagatag acagtgattt aaagactaaa atatatgtaa gagaaaaagt agtagataca
     3601 atagaagtta tttctaaatg tgaagacgaa tgtgatgcta ttatatttac aggatgtgca
     3661 gtttatgaat ttataaaatc taagtatgag ataagtaagc cacattcatt tgtaccgaga
     3721 agtgggacga gtataatgaa ggcattttgg gcaataaaaa gtgcaaatat aaaattagat
     3781 aaattcagta tagatgtagt tgatagatat gttgtggaag atgcacttaa agaatttgat
     3841 attaaagcaa cgagtgtatt ttgtaatccg ttttctttgg aagtaggaga gtctgaactt
     3901 gtagaatggc atataaaatt atttgaagaa aataagacgg atataatgtt aactggtttt
     3961 ggtgcagtat ataatgaatt aaaagaaaga ggctatcctg tatttcgttt acaagctact
     4021 atacaattaa tcaaagaaag ttatgacaag gtaaagagtg aatatgctct aagtaaggca
     4081 agattttctc aaattgctgt tgaaatattg agtttaatag attataaaga gaagatagat
     4141 aactattatt ctgacatgat aaaaaaatca gatatggata agcttgtagt aaactatgta
     4201 agaagcattc aaggttcctt atttttactt ggaagaaatt attatgttgt atttgttcat
     4261 aaaggggtag ctgataatga gtataattat gataagctat tcaatctaaa gagagagata
     4321 aaagacatgg gcttttctct tagtatagga attggtactg gagtaactgc gtatcaggct
     4381 gaaaataatg cacataaagc attaagacac tccatagatt caaaagaaat tggtatatat
     4441 ttggtagatg aagatgaaaa cataagagga cctttagctt ccgaaaatga attaaattat
     4501 tctttgatgc tatctgatga aaaggtaatc gaaatatcta aaaagactgg aatgagttgt
     4561 gagtcagttg caaaaattat tgctataagt gagaatagaa aaagtaagat ttatgattca
     4621 aaagagttag cagagtatct aaatgtaagt gaaagaagtg caagacgtat attaaataaa
     4681 atagtaaatg caggtttagg tcgaatatgt gcaaaagaga ctagtattgg tggaggaaga
     4741 ccaaaaaata ttactgaaat actgttttaa gatattttat aaaactgtat aaatcattat
     4801 attgtataaa attctacaac aaattactat ataaagtatt aaagcttatc atgaaaaaaa
     4861 ttagaacaaa ttattatata aaatactaga gcttgttatt aaaaatacta gaacaaatga
     4921 ttatgtaaaa tactaaagct tgttattaaa aatgctagaa taaattatta taaaaatact
     4981 agagcttgtt attaaaaata ctagaataaa ttattaatat aaaatactag agcttgttat
     5041 taaaaatgct agaacaaatt attataaaaa tacta
//
LOCUS       DAEMHW010000234         5051 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEMHW010000234 DAEMHW010000000
VERSION     DAEMHW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.234"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="KPK67_003408"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="KPK67_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBG2460326.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="KPK67_003409"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="KPK67_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBG2460327.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="KPK67_003410"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="KPK67_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBG2460328.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1245..1670
                     /gene="rplK"
                     /locus_tag="KPK67_003411"
     CDS             1245..1670
                     /gene="rplK"
                     /locus_tag="KPK67_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBG2460329.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1741..2439
                     /gene="rplA"
                     /locus_tag="KPK67_003412"
     CDS             1741..2439
                     /gene="rplA"
                     /locus_tag="KPK67_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBG2460330.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2491..2619
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2662..3168
                     /locus_tag="KPK67_003413"
     CDS             2662..3168
                     /locus_tag="KPK67_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBG2460331.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3226..3591
                     /gene="rplL"
                     /locus_tag="KPK67_003414"
     CDS             3226..3591
                     /gene="rplL"
                     /locus_tag="KPK67_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBG2460332.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3983..4771
                     /locus_tag="KPK67_003415"
     CDS             3983..4771
                     /locus_tag="KPK67_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG2460333.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
BASE COUNT         2035 a          589 c          960 g         1467 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa acaatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aagtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacctattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagttat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagacacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag aaaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atattataga aaaatctgag gaggtgcgct caaaatggct aaaaaagtta
     1261 taggtcaaat aaaattacaa atacctgcag gaaaggctac tccagcgcca ccagttggac
     1321 cagcattagg acaacatggt gttaatataa tgggattcac aaaagaattt aatgctaaaa
     1381 ctgcagatca agctggaatg ataataccag ttgttataac tgtatatcaa gatagatcat
     1441 tcagttttat aacaaaaact ccaccagctg cagttttaat taaaaaagca ttaaacttaa
     1501 aatcaggttc tggagaacca aacaagaaaa aagttgctaa aatgacttca gctcaagtaa
     1561 gagaaatagc tgaattaaag atgcctgact taaatgctgc atcagtagaa gctgctatga
     1621 gtatgatagc tggtactgct agaagcatgg gtgttgtaat cgaagactaa ttaaaagata
     1681 cacttttttt tataaagtgg gaggctagaa gccgaaatta accacgagga ggaaaataaa
     1741 atggctaaaa aaggtaaaag atatgcaggt gcattacaaa aagtagatag aactaaattt
     1801 tatgatgcat cagaagcatt aacattagtt tcagatatag ctggagctaa atttgatgag
     1861 acagtagaag cacacattaa attaggtgtt gactcaagac atgctgacca acaagtaaga
     1921 ggtgcagttg tattacctca tggaactggt aaaacaaaaa gagttttagt ttttgctaaa
     1981 ggtgaaaaag ctaaagaagc tgaacaagct ggtgctgact ttgtaggagc tgaagaatta
     2041 gttcaaaaaa tacaaggtga aaactggttt gactttgata tagtagttgc tactccagat
     2101 atgatgggtg tagtaggtag attaggtaga gtattaggac ctaaaggttt aatgccaaac
     2161 cctaaatcag gaacagttac atttgatgta gctaaagcta tagatgaaat aaaagctggt
     2221 aaagttgaat acagattaga caaaactaac ataatacacg ttccagtagg aaaagtatca
     2281 tttggtggag aaaaattaac tgaaaacttt actgcattaa tggatgctat aataaaagct
     2341 aaaccagctg ctgctaaagg acaatattta agaagtataa cagtagcttc ttcaatggga
     2401 cctggagtta aaataaaccc agcaagaaca gctgaataat aaaactgttg acacaggttg
     2461 acaaaaatgt tattatatca cttgtcaatg taaaaaagaa tagatttgcc gtagatagtg
     2521 ggtgcgaaag cttaatatcc cgccgaggtt ttagataaat tgagattact aaagcttttg
     2581 atgtctactt ttggcaacaa aagcttttta taatgttgcg gtaaatacta agcctatttg
     2641 gtgaaggagg tgcacatcta tatgagaaaa gctatagaaa ttaaatcaga agttgtttct
     2701 gagatagttg aaaagttaca aaaatcttct gctgctgttg ttgtagatta caaaggatta
     2761 acagttgaag aagtaacaga gttaagaaaa caaatgagag aagctggtgt agattacaaa
     2821 gtatacaaaa atactttagt tagaagagca gctaaagaag ttggtataga gcaatttaat
     2881 gatgaattat tagttggaac taatgccata gcatttggat atgatgatcc agttgctcca
     2941 gctagaatat taaaaggatt catggattct catccaaaaa tgaaattaaa gatgggtatt
     3001 gtggaaggtg cattctatga tgaatctaag attgttgaaa tggctaacat accatcaaga
     3061 gaagttctta tcgctaaatt acttggtagc ttaaaagctc cagtatcaaa cttcgcatac
     3121 ttaatagatg caatagcgaa aaaggcagaa ggtcaagaag aagcttaatt gcttaactaa
     3181 aaaattataa atattaattt gaaataaaat tcggaggtgc taaaaatgac aatagaacaa
     3241 atattagaag ctatagaaaa tatgaaagtt ttagaattaa atgaattagt taaagctgct
     3301 gaagaaaaat tcggtgtatc agcttcagct ccagtaatgg tagctggtgc agctgctggt
     3361 ggaccagctg ctgaagaaaa aactgagttt gatgtagtat taactgacgt tggttcttca
     3421 aaagttggag ttataaaagc agttagagaa ataacaggat taggattaaa agaagctaaa
     3481 gaagtagttg acaatgctcc taagacagtt aaagaaggag cttctaaaga agaagctgat
     3541 caaataaaag agaaattaga agctgctgga gctaaagtag aagttaagta gtttctttaa
     3601 aaagataccc attgggtatc tttttttatt tataaaaaac tataaagaaa gtatttggaa
     3661 ttataaagaa agtattttta taaaataaaa aaaatttata taatagtact aaattaaggg
     3721 tatcatatat ggtgtaatgg tattttaatt taacattatt atcaaattaa aatataaaca
     3781 attgttgtaa tgtctatata taaagtatat aattaaacta aaatgtaaaa ttttacattt
     3841 tagtttaaaa aaatacacga gtgttttata aataaaatgt aataaataat taattaacaa
     3901 atataaattt taaaataagt tagatattta gaaaaatcga aatttgtagg atttaaagga
     3961 catattcaag gaggagttaa taatggaaaa attacaagga aaaattgcag tagttactgc
     4021 agcaacaaaa ggtattggat tagcatcagc agagatatta gcaaaaaatg gagcaactgt
     4081 gtacttagca gctcgttcag aagaattagc taatgaagtt ataaataaaa taagtgcaga
     4141 aggtggttgt gctaagtttg tttactttaa tgctcgtgaa gaagagacct ttacctcaat
     4201 gatagaagag gtagttaaaa aagaaggtaa gatagatata cttgtaaaca attttggttc
     4261 aacaaaccct tctcttgaca aagaccttgt aactggggat acagataatt tctttgacac
     4321 agtaaatact aatttaaaaa gtgtgtattt accatgtaaa gcagcaattc ctcatatgat
     4381 aaagaatgga aaaggaagta tagtaaatat atcaagtata ggttcagtat tacctgattt
     4441 atctagaata gcttactgtg tatcaaaagc agcaatcaac tcattaactc aaaacatagc
     4501 tacacaatat gctaaagaca atgttagatg taatgctgta cttccagggc ttattgcaac
     4561 taaagcagca ttagacaata tgtctccaga gttcataaaa gaatttttaa agcatgttcc
     4621 tttaaatcgt ataggggaac cagatgatat agcaaaagct gttctattct atgctagtga
     4681 tgactcatca tttataacag gggatttact tgaagttgca ggaggatttg gtttacctac
     4741 tccacaattt gcagataata tattaggata attaaattac taatatgcaa tgaaagctta
     4801 tgcattagtt atagtagaaa tgatattttt ataaaggcca tagatataat ttatattgga
     4861 aaggtttgag gttcaaaatt ccaagttaaa tttatttatg gtcttatttt gtatatttta
     4921 ataaattgta tatatttgta taaatgtaat tatttacaat cattttaata tggaattata
     4981 attaaatagg agggatggta cttgtataaa attttttata gaaaattaga cctataaatg
     5041 aaaaatatgg a
//
LOCUS       DAEMHW010000235         5050 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEMHW010000235 DAEMHW010000000
VERSION     DAEMHW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.235"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..994
                     /locus_tag="KPK67_003416"
     CDS             92..994
                     /locus_tag="KPK67_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG2460334.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
     gene            complement(1676..2281)
                     /locus_tag="KPK67_003417"
     CDS             complement(1676..2281)
                     /locus_tag="KPK67_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HBG2460335.1"
                     /translation="MTNWNLWRTLNMEYIINPCSGIKIKITRGQKIEIVDIEGGQVVD
                     FFAEMKDDSTEFISPGVTIDCNESLRLQVGNLIYSNKYRPMFKILYDDVREHDLLHPC
                     CRPEMYDFFYHNGENHPNCFDNINRTLGTSHQIIHPINLFMYTKVNTDGSIEVCEPLS
                     KANDKIILEALENMTLGIAACSVSESSCNSRKCTSIKILVD"
     gene            2651..3220
                     /locus_tag="KPK67_003418"
     CDS             2651..3220
                     /locus_tag="KPK67_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG2460336.1"
                     /translation="MRKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(3533..4870)
                     /locus_tag="KPK67_003419"
     CDS             complement(3533..4870)
                     /locus_tag="KPK67_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG2460337.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
BASE COUNT         1803 a          591 c          841 g         1815 t
ORIGIN      
        1 tgtggagaat tagtaagaca tattttagta tgcaaaattc tatgaagcag gaaccctgtg
       61 actttagtca tgggaggttc agattggaaa tgtgttgcca caagattatt atggattaat
      121 agtgatttta ggtgttattc ttggagttat gggggcattt tacaactggt ttactctata
      181 tgtgcagtct ttatacaaga agattcctaa aattggtacc tttggaaaac tgttgattcc
      241 atttataatg gctggtattc ttgggcttgt tatgcctgat gttctgggga gtggacacaa
      301 tttagttgaa gaacttacaa gtcatgaata tatttttgga atggttgttt tgatttttgt
      361 tatacgtttt atattttcgg ctgttagttt tgggtcaggt gcaccagggg gaatattttt
      421 tccattgctt gttttaggag catttatagg tggaatattt ggtatggtag gtgttaaagt
      481 ttttggaatg aatcccgatt atgtaaataa ctttgtgctt ttggctatgg caggatattt
      541 tacagctatt gtgcgtgccc ctttgactgg tatcatatta atttttgaaa tgacaggttc
      601 tgttagtcaa atgctttcac tatcagtaat ttcaattgtg gcatatattg tggctacact
      661 tatgaagtct aaacctattt atgaaagtct tcttgagagg ttgcttgaaa aaaatggaga
      721 acctgtacat aaggagaggg gcgaaaagat actagaccag ttctctgtta tgtttggctc
      781 tcctgttgcg aacaaggcaa ttcaggatgt acagtggcct gaaaattgtc ttttggtagc
      841 aattcaaaga ggtggagaag aaattattcc tagaggtaag acaattctta gaccaagtga
      901 tgtaattgtg actatgacag atgagagaga tagtggtgct gtgtatgata aaatggaaaa
      961 tttgtgtaaa gaacatacgg cacatatttt ttagggataa tgatgaatgt ttaataatga
     1021 tagaatattt aatgaagatt tgataattaa aaaattggta atttgaaaga gagttaagga
     1081 attaatataa gaaaatgaaa atgtgatatc ttagatgtaa aagagtttga agaaaataat
     1141 tgtatatatt tgttaatgag attatatgga ctaatggtat atttatactg tgctaaaagt
     1201 tgcactattg tatgaggaat gggaataaag ggtttgaaaa aataggtaga agatttttca
     1261 gatattctta ttgggatatt ttaattaata tattttaatt gcgatatttt gggaagaata
     1321 gaatttattt ataaatgaaa ataattaaaa ggggttaaga ttagatgaat actagtaagg
     1381 gtattatgag ataattaaca gttaacagct agagtggcta tgaatacagt gtgtattgca
     1441 agaaaaaggt acacactgtt ttttcgttct gttacgcaat aaaatgctat tttttagagt
     1501 atgagtataa aatcctcgct catttgtaat tgtgtctgaa aaacatcata gaatcaaggg
     1561 atttttaccc ataaatacgt aacaaaccat aatgaattga gagcgcaagt ggttaccctt
     1621 ttgcgccttt gacgtcattt tttaattttg atattttttc gataaatgaa atttgtcaat
     1681 caaccaaaat cttaatagaa gtacattttc tgctattaca agaactttct gaaacactgc
     1741 atgcagcaat ccctaaagtc atgttttcca atgcttcaag aataattttg tcattggctt
     1801 ttgataaagg ttcacatact tcaatactcc catctgtatt gacttttgtg tacataaaca
     1861 aattaatggg gtgaataatt tgatgggaag ttccaagtgt gcgattaata ttatcaaaac
     1921 agtttggatg attttctcca ttgtgataaa agaaatcgta catctcaggt ctacaacatg
     1981 gatgtagcaa gtcatgttcc cttacatcat catataaaat tttaaacata ggtctatact
     2041 tattagaata tattaaattc ccaacttgta atcttaatga ctcattacaa tcaattgtaa
     2101 cacctggaga tataaattca gttgaatcat ctttcatttc tgcaaaaaaa tcaacgacct
     2161 gtcctccttc aatatccaca atttcaattt tctgccctct agttatttta atttttatac
     2221 cactacaagg attgattata tactccatat ttaatgtcct ccataaattc caatttgtca
     2281 ttctgttcta tgttcccatt ttagcacata ctctgtttct tttctatctg tttcttaatg
     2341 ttgataacat agtctatcat tttttaattc tcataaagat gttgtagttt ttttaagatt
     2401 acagagtttt tttgctccta tttggtattt aagcaatctt actgtattta gaagtaagaa
     2461 aaaatgttct aatatttaaa gaaatacaca agtgtaagaa gggtaaaaat ctgttataat
     2521 acattaaaaa ctaaatttag aaaaatttac ataattagaa aaatgatttg ataaaaaata
     2581 aagttaaata ttaactaagt aaatttgaaa aagaaaaaat ataaagtaca aatttaaaag
     2641 gaggtaacta atgagaaaga agttggaaga tatgacttta gaggagttat ggaacttgtt
     2701 tcctatattt ttgactactc acaaagaatg ttggaaaaat tggtttaaag aagaacaata
     2761 tagactaact ggaatattgc caatgcaaaa aattgttaga ataagtcata ttggaagtac
     2821 ttctgtgagt acgatttatg ccaagcccat tgttgacata ctgattgaga taattcctga
     2881 ttataatatg aaagatatta aagatttgat tgagtcgaat ggatatattt gtatgtctga
     2941 gagcagtagt aggctttcac ttaataaagg gtatacagta gatggatttg aagaacgagt
     3001 ttttcatttg catataaggt atgtaggtga taatgatgag ttgtttttta gagattactt
     3061 aattgaccac ccagatgttg caaaggagta tgaaaaactt aagttggacc tttggaagaa
     3121 gtatgagcat aatcgtgatg cttatacaga tagtaaacta gattttgtta gggagtttac
     3181 tgaaaaggca aaggtgttgt attctaatag atataattag aaataggtac aattagatat
     3241 ttatcttata tattgtatta tattttttaa gaaagtaatt aatagaaaaa gggcttgttt
     3301 caaaatggag agataaatcc ctttttcatt gttgaaagga taaaaatata tttgatttca
     3361 tgtaaaaaag agtgcctcat caactaaaaa gtttattttg agacacacac atttttacta
     3421 aggtaaataa aaatatcaca tttgattaaa ttttaagtta atgaatatat gtgtaaatat
     3481 ttaattataa gttattataa ttttacatag tatcaaacaa tctaatttta gactaaaata
     3541 atctaatata aagaaataaa gaaaattcac tctcagtata agagatatcc aattttccat
     3601 tgtacttatt aacaatttct ctaatattct caataccata tccgtgagaa ttactatctt
     3661 ttttatctga aataaatagt tcttgttttc tattgatagg atttaccttg ttgtttgtca
     3721 ataaatagct aaaatactcc ttctcacaac gtgcctttaa taccactttt ctatttgaca
     3781 aatcaggaat tttcaaagat gcttctatag cgttatctaa agaatttgaa aaaatagtac
     3841 ataaatctat aggttctata cccaagtgat tgtctatatc tatattaaag tgcacatcaa
     3901 caccattttc ttccaaactt gtatgacgaa gatttaaaat tgagttaaga gcttggtttt
     3961 tgcaaaattg tttgccacta agagtaccta tgcctatttt taactgatta aaatactttt
     4021 tagctccagc aatatcttca atttctaaat acgattcaat catttgaaag tgattattca
     4081 tatcatgtcg cattttgcgt atttgaattt gttgattttc tagtaagtta tagtattttt
     4141 cttttaggtg atttgcttgt ttttctatct tcatctttac attttcaatc aatggctgta
     4201 aaagaagtat cacaccgata tttgccagaa ttgctgacaa gatgattaga taagtgccat
     4261 aaggtaagtt gcctaagata atattcttat tactacccat agggattaaa ttagtaggtg
     4321 gcagaataat agcaattaat attgataaaa atgaagatat acataccata tcaataaaaa
     4381 tccatatttt atttgataag tattgtttta tattgaccaa tctattcttt aggaaaatat
     4441 gtattaaaaa ccaaaataat aatttaatta gagcagaaat tatctggact attgaaagta
     4501 ttaaaatata ctcttttgag tgtataagtt cctcatatat agggttgttt ataaaaagaa
     4561 aattaagccc aattataata ggataaagta tcatgacaac tgatattata ggaattaatt
     4621 tgcttttact acatattata agtagtataa aatatccgaa tagtgtaaac aaaatattta
     4681 cggggtcgga aagccatatt atcatttgac taataatgga gtagcccaaa atcaatatga
     4741 ttttgaaaat ccaatgattc ctaaagtcta taaaatagga agtcaactta taaagaaaaa
     4801 aggctatgaa aatattgatt aatattagaa taaaatttat gcttgtagag attaaagaat
     4861 aagtgcccat ttaatatacc tttctattaa ttaagattct ggaaactaag ttttaaaaaa
     4921 ttaaatctta aaaattaaaa ttctggaaat tagatttcaa agaattgatt ttgaaaaaaa
     4981 aattctagaa attaagcttc aaaaaattga gttttaaaag attgaaattt taaaaattaa
     5041 gtttcaaaaa
//
LOCUS       DAEMHW010000236         5029 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEMHW010000236 DAEMHW010000000
VERSION     DAEMHW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.236"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            574..1569
                     /locus_tag="KPK67_003420"
     CDS             574..1569
                     /locus_tag="KPK67_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2460338.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            1547..2410
                     /locus_tag="KPK67_003421"
     CDS             1547..2410
                     /locus_tag="KPK67_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG2460339.1"
                     /translation="MSIMSNYNEASSNDSMFYKFKQSIRRYYEVSKINFKVQLIWRFD
                     VVVNMAFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            2407..3201
                     /locus_tag="KPK67_003422"
     CDS             2407..3201
                     /locus_tag="KPK67_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG2460340.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            3486..4865
                     /locus_tag="KPK67_003423"
     CDS             3486..4865
                     /locus_tag="KPK67_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2460341.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTVFCIF
                     KYKNRYKYM"
BASE COUNT         1810 a          499 c          813 g         1907 t
ORIGIN      
        1 aaaatattac agtaaaataa ccagttttaa tatacgtatt atatttttaa tttataaacg
       61 aatattataa atgaaattta aattcaaccc tgaagaaata agctaaatgt attagtatat
      121 aaatccatac ttgaacataa ataaccttta tgatatgata tcaataataa tattatgaga
      181 ggagtgcctt gatgtcggag ttatataatg gtaaccaaca attaaatagt tggagacaca
      241 tatattctgt ataatcagaa attttaagtg tatttttaat acatttaatt tttgtgtctt
      301 attctgcctt tatagataac tgacatatga caacaaatag tatttaaaat atatattact
      361 ttgttaaatt atggtgtagt aggctttaat ctgctacact tttattaatt ttgtatagta
      421 ttattttgat aactattttg aaatagagag tattaatttt tctagtccac aggagttttc
      481 tgtttttgtg ctgttaaata taagcacaga aaaaggcaga tatatacctg tggacttttt
      541 ttatataaat tatgaaatat ggaggtagtt aaaatgagta taattgaact taatgaactg
      601 actaaatctt atagaagatt tgagaaaaag gcaggattta tggggagtgt aaagtcttta
      661 tttaatagga aatatacaga aaaaacagct atatcgtcat ttgatttaaa gatagaagaa
      721 ggagaatttg ttggattaat aggtcaaaat ggagctggca aaactactct tattaagatg
      781 ttaactggta ttattgcacc gtcttctgga gaaatatctg ttatgggtta ttaccctaac
      841 aaattagaaa ataattttaa aaaacaatat gctgttgtta tgggccaaaa aagtcaacta
      901 ttctttgaat tgacaccagc agatacgttt cttttattta aagaattata tgatatatct
      961 aatgaagaat ataaaaaaaa cttggattac tttatcgaat tatttgaggt atctgactat
     1021 ttaaatgttc aagttaggac gctttcttta ggagaaagaa tgaaaatgga actaatagta
     1081 gcccttctac ataatccaaa aatattattt ttggatgaac caactatagg cttagatgct
     1141 atagcccaaa aacaaattag gtatttccta aaagaagtaa atgaaacaaa aggaactact
     1201 attatactta cttctcatta catggaagat attaagagtc tctgtagtag aagtattgtt
     1261 gtagaaaata gccgtaaaat atatgatggt tctacagaga aactatttaa tcattttcaa
     1321 aaatataaaa aaataacagt tagtttttcc aatgaaacta acattgtatt tccaaaagat
     1381 tgtaaagttg ttgaacaaac aaatcataag atatcagtaa ttgtgccaaa agaaagagga
     1441 aatgaaacga ttaaatggat ttttgataaa tatgatgtta aagatgtaat gattgaagaa
     1501 gaagatatag gtagtattgt agagaggata tataagaaag gaagtgatga gtattatgag
     1561 caactataat gaagcaagtt caaatgattc aatgttttat aaatttaaac agagtattag
     1621 aaggtattat gaagtatcca aaattaactt taaagtgcaa cttatctgga gatttgatgt
     1681 ggtggttaat atggcgttta cgattgtaaa aattttattt gcttatattt tatggaaagc
     1741 aatttttggt gaaaataaaa tagtctctgg ttttacattt aactctatgt tgtcttacta
     1801 tataataagc tcatttttag caggtattga tatgtcaaag agtaccagtg aagagatatg
     1861 taatcgtgta aaattgggca ccttttcaaa gtacatgatt attccaatta atgttcaaaa
     1921 ttattttatt gctaaaaatt ttggaatgac atgtttttat tttgtattta atatgatttc
     1981 tgcatttgta tggattttta tatttaaaat agattttgcc tttacatcaa atccagttca
     2041 aatattaatt tcaattttga tgatttttct agggtttatt tttatgatga aactccactt
     2101 ctttattggg attttgtcat ttaaatttct tgagattagt ttttttagaa tgataattga
     2161 aaatattgtt gaatttatta caggaacaat gataccactt gtactcttac cagaaactat
     2221 agtaacagtt atgaaggtat ttccatttta ttatacaaca tatttgccat ctatgttact
     2281 aataggtaga aacagcaatg aggcaataca aggattaatt attctagcat tatgggtttt
     2341 atttttttct gtagtaaata aagttatgta taaaagaatg agaacattat ttgatggggt
     2401 gggtatatga tgaaaaaaaa tttgaaaata atatttatgt tattaaaaat gaaattatca
     2461 aagatgatgg tatttagatt tgattttttt ggagcatttt ttgtagatag ttcattattt
     2521 atattacaac ttctaatgtt taatagtata tattctcatg tagactcaat agggggatgg
     2581 caacaaggag agatgttaat atttatagga actttctcat taataaatgc gataaatatg
     2641 acgattttct tttttggtat ttatgatatt ccacgaaaaa tccaagaagg agaattagac
     2701 tactatatta caaaaccatt agacccacta tttaggttaa catttgaaaa tattaatatt
     2761 ggttcatcac cattaattct agcaagtata cttattgttt tatatggaat atctgagtta
     2821 aactcagaga taactttagt tatgataata atatatattt tcatggtgtt attgatgaca
     2881 ttactttttt atgatgtatg tatcatactt agaacagttt cattttttgt tatatcatct
     2941 tctgcgataa tgagattaga agataattta ttgccaatga atatgaaaat tcctggtgta
     3001 atatatgaag gtgtttttaa agtattattc tatttaatct taccatatgg aattatgtcg
     3061 acaattccga ctcaaatttt ggctgggaca ataacaatta atggattaat atactcaatt
     3121 ttaattgtat ttttgtttac attgtttatg tggagatttt ggaagttagg tatgagacat
     3181 tataaaagtg caagtagtta ataaattagt aaaaataaac aatgtataac tatatataaa
     3241 tacgtttcat atgacactaa actatgtgaa caagtatttg cgctaaaggc actgaaagct
     3301 ataaaattat taaagtaaga atttaaaagt ttctttagta gaaatataat tgtgtaaaaa
     3361 agcaagatta attaaaacta tatccaaata attagatata attcttatgc agaaaggagg
     3421 tctgtaaatg tctaattgtt tggaatatat tttaaaaata attaatgtac agaaaaataa
     3481 acaaaatgag tgatgtatat tattcagaaa atcatgaaca agataatctt tatgagcaag
     3541 ataatttact ggcacaaaga tttaatttat ttactctatt gaagtttgca tttcctacta
     3601 tttttatgat gatttttatg gggttgtata caatcgtaga tactatattt gtatcaagat
     3661 ttattaatac aaatgctcta tcagcaatta atattgtatg tccaattatt aatcttatag
     3721 ttggaattgg aactatgctt gccactggag gaagtgctat tgtagcacgt aagatgggaa
     3781 atggtgatat tgagggagca aataaagctt tttctttaat cgtattatca ggagttgtaa
     3841 ttggtgtaat aattactggt ataggtattg tatttatgga ttatatcatt atatggttag
     3901 gagcaagtga agttttaatt aaatattgtc gttcatatct ttttattatc ttggtttttg
     3961 cttctgcaaa tatactacaa gtattatttc aatgtttatt tgtaacagct ggaaagcctc
     4021 aatttggatt atggctagtg ttaggtgctg ggtgtgctaa tataattctt gactatgtgt
     4081 tcattgttct gttacaaatg ggtattagtg gagcagcact tgcaactgga gtaggatacc
     4141 taattcctac cgtttcaggt atggttttct tttcaaaaaa taaagggtta cttaaatttt
     4201 gtatacctag actagataaa agaatattat ttgaagcttg ttttaatggt tcatctgaaa
     4261 tggtgggtca actatcaaca gcagttacta cttttttatt taatgctatg atgatgaaat
     4321 taattggtga agatggggtt gcagctatta caataattat ttattctcaa tttcttttaa
     4381 cttcgcttta tataggtttc tcaatgggtg ttgcaccaat aataagttat aactatggaa
     4441 gtgaaaataa tacgcagtta aatcatgttt ttaagatttg tatattattt atttcagtag
     4501 catctttatt ggtatttatc ttttctatat catgttcacc aatcattgta gagatttttt
     4561 ctcaaagagg aagccgagtt tatgaaattg caaggtctgg attttatata ttttctttta
     4621 gttttatttt ttgtgggtat aatattttta cttcgtctat gtttacagca ttatcaaatg
     4681 gaaaggtgtc tgcaatatta tcttttctac gtacatttgg atttataact ataggtttat
     4741 tgatattgcc aaatatatta aatataaatg ggatatggtt agcagtacca agtgctgaga
     4801 ttttaacctt atttttaacc gttttttgta tatttaagta taagaataga tataaatata
     4861 tgtagatatt aaataaatag cttagataga tattttatgt tttaataagt aattcaatta
     4921 gtggtagata tatactattg aaaattaaag ttataattga tataaagaat taataaaaat
     4981 aacatataaa agtttaaaag ggagttgaac ttaaagtttg attagaaaa
//
LOCUS       DAEMHW010000237         4996 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEMHW010000237 DAEMHW010000000
VERSION     DAEMHW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.237"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..611
                     /gene="recX"
                     /locus_tag="KPK67_003424"
     CDS             <1..611
                     /gene="recX"
                     /locus_tag="KPK67_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBG2460342.1"
                     /translation="KRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNEINEEELKPIL
                     DDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKSYNLVNDSVLA
                     QKIVNTNVNLNKCGKNRIKQNLYNKGINRSTIDEAVSELDKDTEFENAMYLAKKRYER
                     VKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            764..2767
                     /locus_tag="KPK67_003425"
     CDS             764..2767
                     /locus_tag="KPK67_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBG2460343.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGCFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNILPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLSFDKSMLELTKYSD
                     IFIQNSHELIHIDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFNSTPPSSKMPIASRTAIVLLDNGI"
     gene            2941..3582
                     /locus_tag="KPK67_003426"
     CDS             2941..3582
                     /locus_tag="KPK67_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2460344.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMQVKNPEIAAQIFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            3675..4358
                     /locus_tag="KPK67_003427"
     CDS             3675..4358
                     /locus_tag="KPK67_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460345.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(4535..4846)
                     /locus_tag="KPK67_003428"
     CDS             complement(4535..4846)
                     /locus_tag="KPK67_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBG2460346.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
BASE COUNT         1912 a          654 c          778 g         1652 t
ORIGIN      
        1 aaaaaagaaa tgatgataga gttaatattt atgtagatga taaatttttt attgctatat
       61 ttaaagaact tgtttataca tttaatttaa aaaaagggaa tgaaataaat gaagaagaat
      121 taaaacctat tttagatgat gaaatgtata taaaagctaa aaataaagca ttaaatattt
      181 tatctcatgc tgaccaatct gaaaaaaaat taaaagaaaa actatcatca gaatttgatg
      241 aaaatataat tgagagggtc atagattttc ttaaaagtta taatctcgta aatgacagtg
      301 tattagctca aaaaattgtt aacacaaatg ttaacctaaa taaatgtggc aaaaacagaa
      361 ttaagcagaa tttatataat aaaggtatca atagaagtac aatcgatgaa gcagtatctg
      421 aattagacaa ggacactgaa tttgaaaatg ctatgtatct agcaaaaaaa aggtatgaaa
      481 gagtaaagaa agaagataaa aagaaaatat atcaaaaaat ttctcaacat ctttcctata
      541 aaggctttga ttatgatatt attaaaagag ttttaaacaa acttttaaat tttgatgaat
      601 acgatatata agtataaata ctagggaggt aaattatgaa attaatcatt ggtggcttaa
      661 gctttttatt aggattatat aatatatatc aattatacag agaagatagt atgagataaa
      721 tattattttt tagatgtaat atttattatg gaggagaaat acaatgaaaa gtcaaaaaag
      781 atttaaaatt ttagatattg atgtatacct acaaccattt gaatctgata taaaaaaacg
      841 aatgaaacat tatgaggata tgaaatcaaa aacattgaaa aactataaag attttagttc
      901 ttttgcaaat gctcatcttt actatggatt tcatcagaca aatgatggat gcttctatag
      961 ggaatgggct ccaaatgcag atagtttgtc actaattggt gactttaata attggaatag
     1021 aaaatctcat cctctaagaa aaattgggtc tggtaattgg gaaatattta ttcctggtca
     1081 aaatatctta cctcacaaat cagaggtaaa ggtacaagtt actgcaaatg gaaaaacatt
     1141 tgaccgtata ccattatata taaaaagagt aattcaaaaa gattttggtg gatttaatgg
     1201 acaaatatgg caaccaaaaa ctccatttat ctggactgac aataattttg acttaaaaaa
     1261 cataacttct ccactaatct atgaatgtca tataggtatg tctactgaaa gcgaatctat
     1321 tggaacttat aatgaattta ctgaaaaaat acttcctaag ataaaaaaag ctggatacaa
     1381 taccattcag cttatggcta ttatggaaca tccatattat gcatcttttg gataccaagt
     1441 ttcaaacttt tatgcaatct catctaggtt tggtactcct gaagacttaa aaaacctcat
     1501 aaacactgct cattcaatgg ggattgctgt tttattggat ttagttcatt ctcatgctgt
     1561 aaagaatact ttagaaggaa taaatgagtt tgatggaagt gaacatcaat ttttccactc
     1621 aggttcaaag ggaaatcatc ctgcttgggg tacaaaactt tttaactacg gtaaacctga
     1681 agtaatacat tttttgcttt caaatataaa attttggtta aatgagtacc attttgatgg
     1741 ctttagattt gatggtgtaa cttcaatgtt atatcataac catggtcttg gagtttcatt
     1801 tgatagttat gaaaaatatt ttagtatgaa tacagatatt gaagctataa catatctcca
     1861 atttgcaaat gaattaataa aagaaattaa acctaattcg ataagtattg cagaagatat
     1921 gagtggaatg cctggtatgt gtatacctat aaaagacggc ggaattggat ttgactatag
     1981 actcgcaatg ggagtacctg atttttggat aaaaactatc tctaacttat ctgatgaaga
     2041 ctgggatttg ggcaaaatgt ggtatgaact tactactaga cgacctggag aaaaaaatat
     2101 aggatattgt gaatcccatg accaagcatt agttggcgat aaaacaatta ttttttggct
     2161 agctgacaag gaaatgtact ggaatatgga aataaactca aataaccatg ttatcaatag
     2221 agcaatatct cttattaaat taattaaatt aataactttt tctctagcag gtgaaggata
     2281 tctaaatttt atgggaaatg aatttggaca tcctgagtgg attgattttc cacgagaagg
     2341 caacaattgg agctataagt atgccagaag acaatggagc ctttcagaag ataacaactt
     2401 aaagtataaa caattattaa gttttgataa atctatgctc gaacttacaa aatacagtga
     2461 tatttttatc caaaatagtc atgaacttat acatatagat aatgataaaa agattttggt
     2521 gtacagtaag ggcaaatatt tatttatatt taatttccac cctaatctaa tacaatcagt
     2581 tgatatttca aagtttcaaa atacaaacta caaaacgata ttaaatactg atatggtaga
     2641 gtttggagga aacacaaaaa aagaaaactt aagagattat gatgtatact ttaactcaac
     2701 tcctccttct tctaaaatgc ctattgcctc tcgtacagca atagtgcttt tagataatgg
     2761 tatttaaata tgagccaaat atattaggaa actgcaaata tagtccagta taaggcaaaa
     2821 aaataaaata attaaagcat ggcaatatac ctgttcaaca tgctaaaaag acacattgac
     2881 aaatcattat gtatttgcta agataaacat accaaatgat tgttttgagg agtgagcttt
     2941 ttgggaagaa aaaaagaacc tgataatact atacaaaaaa tacttgatat ttcacataag
     3001 ttgttcttag aaaaagggta tgagcagaca acgattcaag atattgtaga taatttagat
     3061 ggcctttcaa gaggtgctat ttatcatcac tttaaatcaa aagacgaaat aattaaggca
     3121 atcacaagtc ggttgtataa tgatggattt tatgggtatg aggtaaaaaa tgatgaagga
     3181 aagaccgcat tagaaaaatt acaatatagg ttgcttcatg cttatgaaca acacaataaa
     3241 caagacattt ttttaatgtc atcatcacta ttgcgtaatc ctaagtattt agctacgcag
     3301 tttgatgaga ttataaatga tattgtgcct tggataaaag cattgattga agaaggaaat
     3361 caagataatt ctatgcaagt taaaaatcca gagattgctg ctcaaatatt tggttttctt
     3421 ctaaacacat ggtgtattcc aacagtttat ccaggtaatc cacaacagat gatagaacgt
     3481 tttactttgt ttggtgagca agctgctttt atgggtattt cttttatgaa tgataagata
     3541 atgtgtgaat atcaaaaatt tgttaataaa ttttctaaat agtaaatata aaactgcctt
     3601 aatatcggca gttttatatt tacacaaaac aaacatttag ttggtatttt taaaaattta
     3661 atttatttca gtacatgcaa aaggaggttg ctatgaacaa actaattaaa gattgtgtaa
     3721 acttttttaa tgattgtggc ttttcatatt caatatgtgg tggatatgcc ctagaattat
     3781 ttgcgaatat gcatattcgc ccacacagtg atattgacat atggatttct gacacagata
     3841 aagaaaagag cattcagtat atgcttaatc aaggatggga tatatatgag cccttgggta
     3901 attatttatt acgacctatt atcgacctta acaagcaaca tatagagcaa atttgtgttt
     3961 ttgcgattaa gcctgattgc tcatttattg aattaaaaaa agtcgaggat gataactata
     4021 aaatgaatat acagtgtgac gaacagttac agttcgattt tattgaattg atatataatc
     4081 cacagaaaga tggaaaattt cttttttccc aaaatcaaag aattataaga gatatggata
     4141 aggccagact gataacatca gataatatcc catatatggc tcccgaagtg gttttgtttt
     4201 gcaaatcaat atacatagat aggcttggtt atcaaaaaga cttcgatgta acagtcccac
     4261 tattatcaca agagcaaaga gattggcttg taaatgcttt aataacagca tatccagatg
     4321 gacatgcatg gattgaccaa ttggcaaatc agtattaagc cttttagctc ccgaatatga
     4381 cattatgata atccacactc taacagtttg ttggtttttc tagtatataa cactcatatt
     4441 aaaataaaca ctaaaagcca aataacaatt taaatacaaa atgttatttg gctaatttag
     4501 tttgatacta tttaactatc ttttacattc agttctataa tttccaactt ttaatttgtg
     4561 gtattatatt catcttaaca gtttgattct ttaaagcttt tctaaatttc tcagttgaag
     4621 aatctttaaa cattttacta gaagcactct tgaggtcttc tatagattcc caatgttgta
     4681 tcataatcca cttttgagac ttttcatcat ataaaagctc agtatcaata aaaccagatt
     4741 gaatagaatg aaattttaat tctaagtcat ttacaatttc tataaactct tcttttgata
     4801 aattttcttc cacattaaac tcaactattt ctgtaataat tttcatatgt aaaacctcca
     4861 atttattttc ctatatcata tcaaaaaata aattggaggt attgtaaaaa atcgacagta
     4921 taattataaa tagctgtact ttatattttc tttaatcgtc ttattctcac cactaaattt
     4981 aattggtgac gtccct
//
LOCUS       DAEMHW010000238         4967 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEMHW010000238 DAEMHW010000000
VERSION     DAEMHW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.238"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            50..1117
                     /locus_tag="KPK67_003429"
     CDS             50..1117
                     /locus_tag="KPK67_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBG2460347.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKDLIGKDPLDIETIHTIMDGIIICNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            1136..2296
                     /locus_tag="KPK67_003430"
     CDS             1136..2296
                     /locus_tag="KPK67_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG2460348.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREALIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            2309..4405
                     /locus_tag="KPK67_003431"
     CDS             2309..4405
                     /locus_tag="KPK67_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBG2460349.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSAKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHERKDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGSVNTSLGNGRLV
                     FDNGLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(4564..4908)
                     /locus_tag="KPK67_003432"
     CDS             complement(4564..4908)
                     /locus_tag="KPK67_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG2460350.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
BASE COUNT         1888 a          569 c          923 g         1587 t
ORIGIN      
        1 ataaaatttt aaaaattgaa ttataacttc aaaaaataag gagggtaata tgaagataac
       61 agatatcaaa tttgaaaagc ttagaattaa attaaaaaaa cctgtagtag tttcatttgg
      121 agtaattgaa tatggagaaa gtgtaatttt aaaaattgaa actgatgaag ggtattgtgg
      181 atttggcgaa gcagcacctc ttgcagcagt tacaggtgaa gttttggata atgttctttc
      241 agtattgctt atgtttaaaa aagatttaat aggcaaagac ccactagata ttgaaaccat
      301 acatacaatc atggatggaa ttattatatg taatacttct gcaaaagcag ctattgacat
      361 agctttatat gatattaaag gaaaaattat gaatgcaccc ctttataaag ttcttggagg
      421 atttgatagt aaagtccaaa cggatattac tataagtata gataagccag aaaaaatggc
      481 agtagaagct ttagaaagag taaaagaagg ttttaggata ttgaagctta aagcaggtat
      541 aaatcctgaa gacgatattg aagctgtaaa gttaattaga gaagccgttg gagagagtat
      601 aagaattaga attgatgcaa atcaaggatg gaacgtaaat agcagtataa atactatcaa
      661 aaagttagaa gaatttaatg ttgatgcaat tgaacaagca cttccacatt gggatttaga
      721 tggtactgct tacataagaa ataaaagtaa tacaaaaatt atgatagatg aatcattaca
      781 ttcaccaata gatgcaatta aggctataaa aaagaatgca gttgatacat ttaatattaa
      841 gctgatgaaa tcaagtggaa tatatcctgc tataaaaatc aacaacatag ctgaggcaag
      901 tggagtaaat tgtatgttag gatgtatgct ggaaacgaga attggtataa cagcagcagc
      961 aaatttaata gcatctaaaa agaacataac agaagctgac cttgatagtt ttatgttttg
     1021 tgaagaatta gaaggtatat ctggaggatt tgtaatggat ggagatatta tgaatcttgt
     1081 caataaacca ggtttaggaa tagaagtaaa cttatgataa atttggaggt taaatatgaa
     1141 tacacttttt tcaaatccag gtagtatact tgctgtaatg acatcaatga ttgcattggg
     1201 tttttactta caaagataca aagccattaa aagcttagga ccagcactta caataatcat
     1261 aatgggaata atactttcaa acttaaaagt agttccagta agtactgagt tatatggaac
     1321 tatttcaaca tatgctatcc cagtttctat gactataatg ttgatgagtg ttgacttaaa
     1381 agaaatgaca aaattatcaa gagaagcttt aattgcaatt tttgtagctg tattaactgt
     1441 aagtatcatg gcatttttat ttggattagt atttgcagaa aaaatatcag aaggttggaa
     1501 ggttgctgga atgtttgtag gtacatacac tggtggaagt gctaatctta cagctattgg
     1561 aacaggtctt aatgtaagta gacaaactct tgctgcagca aatgcagcag actatgtaat
     1621 tggagtacca acattgatat ttatgtttgc tctaccagca atattaaaaa attcaaagaa
     1681 gtttaaaaaa ctttggccat atcatgtaga agaatcagaa ttagaggatt gtcaaaatga
     1741 agaatttatg gagtcaaaag aatggagtat taaagatata gcttggatgt tggctatagg
     1801 ttttgttgtt acagaagttt caacaatact tgctggatat ttcaattcaa gttttagtag
     1861 tgcagcaaga atacttttag ttacaacaat ttcaattata attgctcaat taaagccagt
     1921 aaagaaacta aagggaaatt tagatttagg attatttgtt gcattattct ttttatgtac
     1981 aataggtttt tcagtagata taaaagagtt tttaggttca acttttacta taacacttta
     2041 ttgttttagc ataatttttg cttcatttgt tttccattta ggcataacta gacttttaaa
     2101 gataaagtat caatatgtaa tactttctat agtaggagca attgctgatg gtcctacttc
     2161 agctttagta gctgcttctg caaagtggaa ttcgcttgta agtgtagcag tcgtaatggg
     2221 agtaattggt ggagtattag gaaattatgc aggtatatca gtggcatatg caataaagat
     2281 gtctctagga ttataggggg gagtttaatt gataaaattt aaattataca tatctggact
     2341 atacagtgga aatgtaatct ttgatggaga tttattaatt gaaaagttga atccttttac
     2401 aaataaaata gaaagtttaa aaccaataag caaagaagaa aatacatatt atttaaatct
     2461 tacaaaaata gatttaaagt ctttatttaa taactttgat gtatatacta aaagcttagt
     2521 aaacacagat aaagacacag ttataaataa cttaggagaa acgcattcta aacttaatga
     2581 atatatatgg gttcaaagaa ataaaaagtt cccactagat ataattattg tagataataa
     2641 aatagtaggt tttatctgtt tatccagaga aacttgtact atattaatta tggatggata
     2701 tgaagagtat actgtattaa aagaatggga aaaaactcat aaaaatgaag agatatattc
     2761 tataagattt ggtggaaatt atatgattga tatgaaagat ggtataaaac tttctacaga
     2821 tgtatatctc cctgattttg tagattctgc caaaaaagca ccgacaattc ttatgagaac
     2881 tccatatgga aaagaaaatg ataaagaaat atattataaa tatgtacaaa gaggatatgc
     2941 tgttgtaata caagatgtta ggggaagaaa tgaatcagaa ggtaagtggg aacctatgat
     3001 tcatgagagg aaagatggag atagtaccat aaattggata gtatcacagg aatggtcaag
     3061 tggaatagtt gggatgcttg gagcatcata tcttggttat gtccaatggg cagctgcatc
     3121 ttcaggcaat aagcatctta aagctttagt tagtatagta acatcaggaa gcccttttat
     3181 tgatatacca agaaaaggag gagcttttgt atctggtatg cttgcatggg catttatggt
     3241 atcaagaaat aaagttgata gaagcaaaat ggtaagagat gattgggatg atgttttaaa
     3301 catacgacca atagaaaata tacctgttga agctcttgga tatagaattg aatttctgga
     3361 agaatggtta aagagagtag aaaaagatga gtattgggat ttgatggatt ggcatttaca
     3421 aaaagataaa ataaatgtac ctgcattggt tgtatctggg tggtatgatg ataatagtat
     3481 gggaactaca gaggccttag atgttataaa ggactatgaa aaaggaaaga gaaaggctat
     3541 tctaggtcca tggatgcata attcaaatac tttaagagat ataaatggta tttctttagg
     3601 aaatagttca ttaagatatg atttggatta taattatcta ttatggtttg ataaatactt
     3661 aaaaggtata gaaaataaca tagatacgac tgctccagta gagtattact ctgtaggttc
     3721 taataagtgg aagacagaag aaaattggcc aataataaat aagatagata aaagtatgta
     3781 tctaataagt gatggaagtg ttaacacatc tttaggaaat ggtagattag tatttgataa
     3841 tggtttagaa gaaaagtatg atagttatat atataatcca aaagacccat cagtacaact
     3901 aattgatatg tcagagaatg aagttggagt tccaaataac tacaaagatt tagaaaaaag
     3961 aagtgatatg ttgtgttata catcagatgc ttttagtgaa gaatttactg taacaggtga
     4021 tataaagtta gagttttttg catcaagttc tgccaaagat actgactggg ttataaaaat
     4081 tatggacgtg gatttagatg gaaattcaat taaattagct gatggtatac ttagtgctag
     4141 atttagaaat agtttttata agtcagagtt tatggaagaa ggagagattt ataagtttac
     4201 gattataact tcaaaaatat caaacacgtt taaagttggt cataaaatta gactagatat
     4261 aacgtctagt gctaaaaact ttattttcca aaatagcaac acgacagagg gatataatag
     4321 tattgaatat atagaagcaa agaatacgat ttatcatgga ggtaaatatc catctaaatt
     4381 gatattgcca atagagaata agtaaattta tatagattaa atagtgagga ggtgtctcaa
     4441 aatgaatttt ttagttcgtg agacactttc ttttatacga aatcaaatat attttcatca
     4501 tttcaacaat gaaaaagagg cttatctctc tgttttgaga tagcctccta taagctaaat
     4561 tgattatttt gtagctgcaa caacgctcat ttcaactaac aaatgctctc tagcaagtct
     4621 agcttcaaca caagctctag caggttcaaa tcctttttca acccaagcat cccaaacaga
     4681 gttcatagct tcaaagtctt tcatatctct taaataaata gtaacagata gcaaatgttg
     4741 cttatctgaa ccatatttgt ttaataaatc ttctatttta gctaaaactt cagttgtttg
     4801 ttcaattact cctccttcag catgagtttg tccacataaa taaactgtat tgttatgaac
     4861 tacagctcta ctcattcttc cagttccttc atatctcttt atttccatat tttaaatacc
     4921 tccatagtta aaaaatctat taaaaatcta aataataaat taaaaaa
//
LOCUS       DAEMHW010000239         4966 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEMHW010000239 DAEMHW010000000
VERSION     DAEMHW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.239"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            670..1701
                     /locus_tag="KPK67_003433"
     CDS             670..1701
                     /locus_tag="KPK67_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG2460351.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1834..3132
                     /locus_tag="KPK67_003434"
     CDS             1834..3132
                     /locus_tag="KPK67_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBG2460352.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPSNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(3246..3491)
                     /locus_tag="KPK67_003435"
     CDS             complement(3246..3491)
                     /locus_tag="KPK67_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG2460353.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3639..4505
                     /locus_tag="KPK67_003436"
     CDS             3639..4505
                     /locus_tag="KPK67_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBG2460354.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4509..>4966)
                     /locus_tag="KPK67_003437"
     CDS             complement(4509..>4966)
                     /locus_tag="KPK67_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBG2460355.1"
                     /translation="TNSQITQVVLETEMMNYFSLQQLLSQLMESKFLTTYKDSDREYY
                     ALTQRGVESLEYFFNRIPTSVTEKIDKYIDSNKENLLADTQVKSSFVKQSDNEFIVNL
                     RVIENQSNLIDLNLNVSSEKQAKLICNNWKNNASYMYAEVIDLLIRDIH"
BASE COUNT         2004 a          495 c          792 g         1675 t
ORIGIN      
        1 cgacatctta tagtttattg aacctatagt aatgaattaa taaatagttt aattctatga
       61 aaagatttcc ttttatgcaa tttatttatt gaaatatgat ataatatata aaggtgtaag
      121 tattagttgt taaaataata tttatattag tttttatgca tacttggtat aagtttttca
      181 tatattgatt aaaattgatg ttgatttatt atttttagta ttttttcacc ttttgattca
      241 tactttgtag aatggtgtat tttctaaaac tgattataag acaagttcat tcaaattagc
      301 atttttatat catataaata attgttcccc ctaaataaga tttagatgta tagtattaaa
      361 atcaaaaagt ataaaataaa attgatgctt aatcatagat aaatattaat aaaatataca
      421 aggggattgt ctaataaaaa gataatctcc ttttttacaa ataattctaa attgtgatat
      481 aaatctaaaa attttatctt aacatttaac catttaatga taataattga tataataaat
      541 ataaattaaa tatatggtgt acttatttta tgtttttagc aagaaatata gcatttaaaa
      601 gataaattgt gcaacttaaa tcaaagttta aaatgaacta ttaacataga tatattaaaa
      661 acttttgtaa tggagaatat atgtgatatg aataataaag ttaaggttgc agtattagat
      721 acaggaatag acaaagaaca tgattactta aaagataatt tagttggagg aattgctttt
      781 gaatgtatgc atgattatat ctttatatca gataaatttg atgatgaaga tggtcatgga
      841 actgcttgtg catcaataat aaagaaagag tatgaagatg tagagttatt tgtaataaaa
      901 attttgagaa aagatggtat tacaaatata aaagttcttg aagaagcatt aagatacctt
      961 ttagacacta acataagatt aattaattta agtttatcag tcataggagt agaaagtgtt
     1021 aagggcttat ttgaagtttg ttatgaattg tttagaaaag gaaaaataat agtatgttca
     1081 ctcgctaatg attttgactt aagttatcca gctatgttta acaatgttat aggtgtaaga
     1141 ggatttactt tagatggaga tgattcattt tggtacaaca aaaaatatga tgttcaatgt
     1201 gttatggata gcaattcata tataagttgt gatataaata attcctatag attaccacca
     1261 aaatgtaata gttatttatc agcaaagctt actggtaaaa tagctaagat attatcagaa
     1321 aaaccaggca taacaatatc tgatttaaat gataagcttg aatcattagc aactagaaat
     1381 cattgggata gttgtgattt tgatgagtgt agtagaattg taaactttaa attggatttg
     1441 tatgacaaag aaaatgctct tcttatgaaa gttgctgacg ttgttaggga ttgcttagat
     1501 attgaagata ataatgaaaa actttttaag ggtagccttt ttaataagga gataggcttg
     1561 gtttatgata attgttttaa tctgttgaaa aagctagagc atagatttga cataaagttt
     1621 aattatatgg acatctcaaa atatgactta gtttcaatat atacactgac tgaattagtg
     1681 gaaagatata aaaacttata aataaagatt tgtagctaaa tttaatgtag aaggtattaa
     1741 attatatgta ttctttgtcg gtagattaaa agtaaaaaat atactataat tagatagcat
     1801 aagagaaaat gccaatacag ggggagttag ataatgaaat atgaagaagc attagagtac
     1861 atatcacaaa ctaataaatt cggtataaga ttaggattag aaaatatcgg aaagttatta
     1921 gaattattag gaaatccaca agaaactttg aatataatac atgtagctgg aactaatgga
     1981 aaaggttctg tgtgttcttt tgtttctaat atactaagag agtgtggata taaagttggt
     2041 ttatatacat ctccatattt agaaactttt actgagcgta taagagtaaa tggacaaaat
     2101 ataccacagg atgatgttgc tagaataatt gagttaataa aagaaaaaat agagattatg
     2161 gtaaaagaag gctatgcata tccgacagaa tttgaagtgg ttactgcaat ggcattttat
     2221 tactattcag aacaaaaggt agactttgtg gcattagaag ttggtttagg tggtagatat
     2281 gatgctacaa atataataac aaaatcactt gtaagtgtga tagtatctat aagtttggac
     2341 catacaggta tacttggaga tacaatagaa aaaatagcgt acgaaaaggc tggaataata
     2401 aaggaaaatg gagtagtact agtatatgac cagactgatg aagcaaagga tgttataaag
     2461 tcagtttgta aagagaaaaa agctaaatat atagaagttg attttgatga tataaacata
     2521 aaaaaatcag atataaattc tcaaatatac gattgtactg taatgaaaga aacttataga
     2581 gaccttgaaa taaaattgat aggcgaacat caaataaaca attctatctt ggcaattagt
     2641 gtaataaaat atctaaaaga cataaataaa ttagcaaata taaatgaaga atctataaga
     2701 aaaggactta taaatactaa atggccagga agaatagaaa agataaaaga aaatccaatt
     2761 tttataatag atggtgcaca taatgaagat ggagcaaaat ctttggcaaa agcacttgat
     2821 aagaatttta aaggtagaaa attaacctta ttaataggta tgttagaaga taaagatatt
     2881 gatagtgttt tagagatatt gttgccacat tttaataagg taataacaac aacaccaagt
     2941 aacccaagag ccataaattc agatatatta agagaaaaag tattaaaata tgtagatgat
     3001 gtaacatcaa agcatgagat agaggatgca gtaaattata cattagaaac atcaagtgag
     3061 gatgatatta taataagtgc aggttcatta tatatgattg gtacagtgag aactttagta
     3121 aagaaattat aattttataa aataagtata caaataaaaa tataaaaaag agttgtctaa
     3181 aaaatggaca actcttttta tataaaataa gtttttaata attttactga tgtttatata
     3241 agtaattata tagattgacg tatagcttta gataattggt ctgggcaaga agtacctttt
     3301 ccaccacagt caataccatt taatttatca gcaacttcaa gagcattttg gccaattatt
     3361 aagcttctaa gtcctgataa attaccatta catcctccta taaactcagc atcaacaatc
     3421 acattatttt catcaacttc aaaaatcatt tctctacaac atacaccact tggattaaat
     3481 gttactttca ttaaaaagac ctccattaaa tattatttct ttttttaaca ttaataataa
     3541 taccaaatat atgcgtaata atgcaactat aaagcataat acaagctaat gaaataatat
     3601 attattgtag tatatgtatt gtagagagga ggggttatat gaaaatctat atttctaact
     3661 acttatcaaa gagtattagc atagttgatt attctacact tgaactagaa aaagaaatag
     3721 tattagaaga taatatatat ccacaccatt tttgcataga aaaagaaaaa aatttaatat
     3781 acatacctag ttcaagtaat gggatactct atgtcttaga tttaagtaat gataaaataa
     3841 ttgatactgt ctctatagga ggaagtctta gtcaagttgt acttagcgat aatgaattgt
     3901 ttgtggcaaa tgaagattct aatagtatat atgtactaga taaaaacaca ttaaatccaa
     3961 taggaattat tggagttgat aatatgccac atggatttga ctttgataga gaaagcaaaa
     4021 agttatatgt accgtgtata aactcgatag tatgtataga tacaattaat aaaaatatac
     4081 aaaagaaaat aaatatggac tttagagctt ggcatataga acttgataaa caaaagaaag
     4141 aaatgtatat atcaacactt gatggaaagc ttataataat agatgaagta agcatggata
     4201 taaaaaaggt attatatgaa tttttattac cagtagaaat aagatttaat tatagtggca
     4261 aaaaagttta tgttgctgat ttgggttata ataatgtaag aattttagat tatacaacga
     4321 gtagatacat tggaaatatt gaaataaatg ggattcccca aggattagaa atatctaaag
     4381 atgaaaagtt actatttgta tcagatacac aagaaaattc agtaaaagtt tatgaaactg
     4441 caaataacaa attaataaaa gaaatcaagg taggaaaaga gcctacaact ataatttgtt
     4501 tgtaggcttt aatgtatgtc tcttataagt aaatcgataa cttcggcata catataagat
     4561 gcattgtttt tccagttatt gcaaataagt tttgcttgtt tctctgatga aacatttaga
     4621 tttaaatcta ttaagttaga ctgattttct atgactctaa ggttgacaat aaattcatta
     4681 tcactttgtt ttacaaaact agactttact tgagtatctg ctagtagatt ttctttgtta
     4741 gaatctatat acttgtctat tttttcagta acactagtag gtattcgatt aaagaagtat
     4801 tctagacttt ctacgcctct ttgagtaaga gcataatatt ctctgtcaga gtctttatat
     4861 gtagtcaaaa acttagattc cataagctga gataaaagct gttgtaatga aaaataattc
     4921 atcatctcag tctctaatac tacttgagtt atttgagaat tagtta
//
LOCUS       DAEMHW010000240         4912 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEMHW010000240 DAEMHW010000000
VERSION     DAEMHW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.240"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            441..950
                     /locus_tag="KPK67_003438"
     CDS             441..950
                     /locus_tag="KPK67_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBG2460356.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            978..1172
                     /gene="rpmI"
                     /locus_tag="KPK67_003439"
     CDS             978..1172
                     /gene="rpmI"
                     /locus_tag="KPK67_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBG2460357.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKRHKLTGKSAKTKMDL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1228..1584
                     /gene="rplT"
                     /locus_tag="KPK67_003440"
     CDS             1228..1584
                     /gene="rplT"
                     /locus_tag="KPK67_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBG2460358.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1894..2439
                     /locus_tag="KPK67_003441"
     CDS             1894..2439
                     /locus_tag="KPK67_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460359.1"
                     /translation="MNEKLMDCFVNPIKCKLLLEVFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            2426..2599
                     /locus_tag="KPK67_003442"
     CDS             2426..2599
                     /locus_tag="KPK67_003442"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460360.1"
                     /translation="MKGNKSDLHEVEPIYTGNRFMIYICIQSKVSLLGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            2874..4799
                     /locus_tag="KPK67_003443"
     CDS             2874..4799
                     /locus_tag="KPK67_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2460361.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TKWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAAVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
BASE COUNT         2064 a          479 c          940 g         1429 t
ORIGIN      
        1 ttgttatgta gtatagtagt atattagaat acaatataga atttgttgtg tagtatagtg
       61 ttatattaga atataatata ggatttgtta tgtagaatat atagagtcat gtagaatata
      121 aatatataaa aggattttgg gtataaattt aaaaattaat agcaaaatga gtagtataga
      181 catataatga gtagtaggaa gtaattaaat ttttcctaaa caaaaaaaat agttgcaaat
      241 aatttgatta agtgatatag taaataaggt aagaataaaa caaagaagaa gtccgcttct
      301 caccttacgc cggaggttag taaggtaaat ataagttaaa gcttaatttt aatttaagtt
      361 tgtttaaatt tggtaactta tattgcggat gattacgtca tccgcttttt attttataaa
      421 aattttggag gtgtcctaca attagcaaag aattagcaat caatgaacaa ataaaagata
      481 aggaaataag agtcctttca ccaacaggtg aacaacttgg agtaatgcct actaaagaag
      541 ctcaagcaat ggctaatagc aaaaacttag atttggttca aatctcgccg aatgcaaatc
      601 caccagtatg taagataatg gattatggaa agtttagata tgagcaagcg agaaaagaga
      661 aagaagctaa gaaaaaacaa aaaactatag ttgtaaaaga agtaagactt agaccaggta
      721 ttgaacaaaa tgatttaaat acgaaagcta ataatgctat aaagttcctt aaaaaagggg
      781 ataaagtcaa agttgaactt agatttagag gaagagaatt aggacataaa gacataggta
      841 aagaagttat gcttaagttt ttagatataa taaaagagtt tggagaacca acaaaagccc
      901 ctgcatttga gggtaataat atggttgtaa ttatagatcc aaaaaaatag atgattaact
      961 gagaggagga atttgtcatg cctaaaatga aaactcatag aggtgcagca aagagattaa
     1021 agaaaacagg aactggaaag ttaaagagag caaaagcttt taaaagacat aaattaactg
     1081 gaaaatctgc aaaaactaaa atggacttaa gaaaatcaac tttagtaagt gatggagatg
     1141 ctaagagaat agcacaatta ttaccatact aagatacaga ttataaataa taactataga
     1201 agagggatat aaaaggaggt cataacgatg gcaagagtta aaaaagctat gaacgctcgt
     1261 aaaaagcata agaaaatatt aaaacttgca aaaggattca gaggatctag aagcaaatta
     1321 tatagaccag caaatacatt cgtaatgaaa gcattaaaaa atgcttatat aggaagaaag
     1381 ttaaagaaga gagacttcag aaaactttgg atacaaagaa taaatgcagc tgctagaatg
     1441 aatggaatat cttattcaag attaatgaat ggattaaaat tatctggtat tgaagttaac
     1501 agaaaaatgt tatctgaaat ggctatacaa gacccagaag gatttgcaaa attagcagaa
     1561 gttgctaaag caaaattagc ttagtattga atcatgagat gtaaagtctt tgagttatat
     1621 aaataattca aggacttttt tattttttga aaaatactta ttagtgtcat gtacaaccaa
     1681 ttttataatg ataaataaaa taagcaatac aatagaattt tatgagattt attataagaa
     1741 ttgttacaat tatttatgat agctataaaa agtcatagta aaaatgatat tgatgtatgt
     1801 tgatatatgt ataaagcttt caatcaatct tgaattaata ttatcaacat gataatatta
     1861 tcttattaat agtataaata agggggagat aatatgaatg agaaactaat ggattgtttt
     1921 gtaaacccaa ttaaatgcaa gttattgctt gaagtttttt caaataatcg aactacagct
     1981 aaacaattag gagaaaaatt tcaagatata tcaaaggcaa cactttatcg ttaccttaat
     2041 aaaatgttga aagatgatat tataaaagtg gttgaagaaa ataaaataag gggaactgtt
     2101 gaaaaagtat atgctatgaa gttagatata gctgatgatg tgaacaaaat tatagatgag
     2161 aactctggag aagaatatgc tcaactgttt actcaatata tgatgggatt tttaagagaa
     2221 ttttatgaat acgctgaaag aaaggaaaag gatatcttaa aagatggttc aggattttct
     2281 gtctgtccag tatatgctag tattgatgag atagttgaag tgagtaaaaa aattgctagt
     2341 atactagaac ctctaagaaa taactcacat gaagaaggta gaagaatgca ttctatagga
     2401 gtgataatta ctccacctaa agaggatgaa gggaaataaa tctgatttac atgaggtaga
     2461 gccaatttat acagggaatc gttttatgat ttacatatgt atccagagca aagtatctct
     2521 gcttggattg tacttcattg taccatttca tccaatgact tggagtggta caatgaagtt
     2581 ttttaatttt tcatattaat gctaaagaaa tgaactttag accacatatg caattaggtt
     2641 gcattataca attagaccag atatacagaa taaaaatcag tatttttata gcagataaat
     2701 tgactgttat tcttcttaaa aatcatctat tagttattgt tagcatattg aaagaaatta
     2761 tagtaatcta ttgaataaat gaatattgcc cataatcata taaactgcta tagtttaaaa
     2821 taaagcagga cgttaaattt tatagtaatc taaaaattta agagggggat tttatgaaaa
     2881 taaaatttgt tattatgcca ttggtgatat ttttaatggt attatttgtg gggtgtcaaa
     2941 gtacaaaaga tacagaagtt aaagcaaaag atacagaaat taaaatatta gggacaggtg
     3001 atttgcattc aattttgaca gactctatgg tttcatatgt aaatgaggaa agggaaaaga
     3061 ataaaaatct gcttatggta gatgcgggag atttctatgg cacacaaagt agagaaatgt
     3121 gggaatggtc ttctggaaaa aagttaatta atattaaaag aaatggtaga gcagagtatg
     3181 atgatataat taagagtagt aaagatgaag tacctatagt taaagatatg gcaaagttga
     3241 aatatgatgc tgtagttttg ggagataatg aatttgtttc aaatgataag caaagtcttg
     3301 ataaactggt ttctgatttt aaaaataata atatgccatt ggtatcagca aatatatatg
     3361 aacaatctgg agaaaattat gtacaaccat atgtaatgaa aaatattaag acagatgagg
     3421 gcaatgtaaa agttggaata ctaggtctta ctataaaaga agtaggggaa acccttggtc
     3481 ttgaggattt tgaaaaggat aaaaaagcta gagagcttgg agaacaagct gattacaaag
     3541 gaaaattata tgcaaatgat ttagtagaag atgcaaagaa atggataaaa gttatggaaa
     3601 aagaaagtcc agacataata gttgcagttg tacactcagg ggaagaacct aaaacagata
     3661 gaaatccagg aaatagaata aaagagttag caactacagt ggatggaata gatgcaattg
     3721 tagctggtca tactcatgaa aaaatagatg aacacaaata taaaaataaa tctggagatg
     3781 aagtaattgt tacacaacca ggttggcatg gagatagagt atcagagatt aatttcaaat
     3841 taaataaaga gaatggaaag tgggacataa aggaaaagtc tagtaaatta aaaataattg
     3901 ataaagaagt aagtatagtt gctactgcag atttacattc acatttctct gataaattaa
     3961 cagttgacct tgttaatgaa cgtagtaacc caattgaacc tgttgtagta gatgcaggcg
     4021 acttcttaga ccctcaaaca gatgaaatga ctaaatggta taaagagtgg aaaagtataa
     4081 gagataataa tagtgataaa acgattagta gatgcccaat ggtcatggat atgaatcagg
     4141 gaatgtatga tgcagcagta cttggaaatc atgaatttgt atctgaaaaa gatgaatttt
     4201 ggtcagatga acgcctttta gatgctgtta tagaggattt tgaacaggtg gcaatttctg
     4261 tgttatcggc aaatatatat aggcaatctg gtaaaaatta tgttaaacca tatattataa
     4321 aagatgtgga gacgagtgaa ggtaatgtaa aagtaggaat attgggactt actataaaag
     4381 aagtaggtaa aggtcttaaa aatgtaaact tacaagagca atttcaatat aaggataaat
     4441 tgtatgcaaa tgatttagtg gaagatgcaa agaaatgggt aaaagttatg aaaaagaaaa
     4501 aaccagatat aatagttgca gttgtacatt caggagaaga accaaaaaat ccaaaacatc
     4561 caggaaatag aataaaagag ctagcaacta cagtagatgg aatagatgca attgttgcta
     4621 gtcatactca tgaaaaaata gatgagcatg aatataaaaa taagtctgga gaaaaagtta
     4681 tagtgacaca accaggtgag catggtaaat attattctaa aattaccttt aaagtaaata
     4741 aagaaaaggg ttcgtggatt attaatgata aattcagtaa aacaattgag gttgaataac
     4801 tttataaaat ttatagataa agtaaattat ctgatattga catgatatat aagataaagt
     4861 ttctaatatt agtataatgc ataatgtgaa atattttaat aataagagaa ta
//
LOCUS       DAEMHW010000241         4907 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEMHW010000241 DAEMHW010000000
VERSION     DAEMHW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.241"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..871
                     /locus_tag="KPK67_003444"
     CDS             <1..871
                     /locus_tag="KPK67_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460362.1"
                     /translation="IIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEE
                     VKNTIKQISKSIEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVR
                     KLDKQFNILNIKFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNI
                     NAGFALYERSAQDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQ
                     VKLDMMFENSLDIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIED
                     KEIKVIGEDELNE"
     gene            864..1496
                     /locus_tag="KPK67_003445"
     CDS             864..1496
                     /locus_tag="KPK67_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG2460363.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            1825..2982
                     /locus_tag="KPK67_003446"
     CDS             1825..2982
                     /locus_tag="KPK67_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2460364.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFILIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(3129..3764)
                     /gene="lexA"
                     /locus_tag="KPK67_003447"
     CDS             complement(3129..3764)
                     /gene="lexA"
                     /locus_tag="KPK67_003447"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBG2460365.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(3868..>4907)
                     /locus_tag="KPK67_003448"
     CDS             complement(3868..>4907)
                     /locus_tag="KPK67_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2460366.1"
                     /translation="CLLLENKLPVFMKDYFIYLKGSVAVSTRLAYLEDINFFCSYLIE
                     TKELTNADCVKDITEDDFNTIKSRDINLFLGDYCSRYYKNTEKNTLIFENNNRALARK
                     KSSISTLFKFLYRNSQIDNNITDGFNPIKLPKPQPDAIKRLEIDEVAKMLESVETGEG
                     LTEKEKVYWRKTKLRDKAILALFVTYGLRLNELRELNISSFNFSRGEFKIYRKRGKEV
                     LMPINHTCEHVIKDYLQNERTRDELLNDEEKDALFLSLQNKRITAKAIRTLVKKYTSI
                     PLDTTRDNGYSPHKLRATAATSLIQTGFSIYDVQNLLDHDNVTTTQLYAAHKKNVKRD
                     IVKNFEWIEDD"
BASE COUNT         1835 a          614 c          737 g         1721 t
ORIGIN      
        1 aattataggt tactttacta agataaaaga ttgtgaacat gttttgatac aaataatgtt
       61 aaaatctgaa aatttactaa catctgaaat aactcatttt aagtcagaaa gaatggattt
      121 aaaagtagaa gaggtaaaaa atacaataaa acaaataagt aagtctatag aagctattga
      181 aaaatttgat aaaatatcta aagatttaaa tgactttaat gatgaattta tcagtggtat
      241 agaagcttta aaaagccttt tagaaggttc tcaaagttct attaagacat ttgaccagag
      301 cgtaagaaaa ttagataaac agtttaatat tttaaatata aaatttgtaa agctttttga
      361 taaatatgat aatcaagata atataaataa agagatttta tttgatataa aagaatcttc
      421 aaaaaatata tacaatgcca ctgaaagcca attcaaaatt agagattata taaaaaatat
      481 aaatgcagga tttgctctat atgaaagaag tgctcaagat ttattaacta aattaatgac
      541 ccatgaaaat aaaatatctc aaaatcaaaa aatcctttta gatgaaaagt ttaccttaga
      601 tgactcaata aaaaatctct caagtattat tgaaaacttt tctaatgacc tacaagttaa
      661 actggatatg atgtttgaaa attctttaga tattcaagat aagttagatg ttatgtttaa
      721 taactctttt atgaatgatg aagttcctct tgatagtgaa gaattattta aggatgatgt
      781 taataatgta tttaatcctt ttagtgaaga aatttatgag atagaggata aagaaataaa
      841 ggtcataggt gaggatgagt taaatgaata ataaatatag aagaactgtt aacagagaat
      901 ttgaaaagag tagcttttgg ccaacattta cagatttatt atctactgta ttgatggttg
      961 taattcttat cttatttagc tcagaaagta tttcaggttc tgttgaacaa gatttagcta
     1021 agaatgtcaa tgcttctgtt gaagaaactt ttaaaaaaag tggaatacct gtgaaggtag
     1081 ataaatctaa tgggcaagta acttttggtg aaaaaaccat gtttgatgta gatagtgatg
     1141 ttttaaaacc agaggctaaa gaaatgctaa aaatgtttgt tccaaagtac atagagacta
     1201 tttataaaga ttatggtgac tatatatcca aaatagttat agaaggtcat acagatgatg
     1261 tgggaagtta tatatacaac ttagatttat cgcaaagaag agcatatagc gttgctaaat
     1321 ttatagttgg agatgaaata ggtgactata aatacaaaga taaagtgact aagcatatta
     1381 ttgcaatagg aaggtcaaaa gctgaactca tcaaaaatgg tgacaatagc gtgaatagag
     1441 atgcatctag aagagtagaa ctaaaatacg aaataaatat taatcaaaat aaataattaa
     1501 atttttataa aaaaaaagaa ggcagttggt gtgagcttta agccttcttt aaggaaaaag
     1561 tttatttatt tttgttgata cttaacatat tatagttttt agaagcagtt tgcaatatat
     1621 taaacgtaaa tggtcgttta aaatgcataa ctggatacta ctgttgtaaa atatttcatt
     1681 ttatataata taaaaattat acagtgtcaa ttttctatat tattgtacta taatctatta
     1741 tttaaaatat tatgtaaaaa aatatcataa aaacgcatat catgttataa ttactagagt
     1801 attatgaata tggaggcgta gtttgtgata gaaataagta taaaaaataa aaaaaattct
     1861 aaaattaaaa ctttgatact tttattgata gtatctatat cgatttattt aactggttgt
     1921 actacatcag ataatggaaa taatcaatca aataataatg attctttgtc tagtagtata
     1981 tcaagtgata agaatgtaaa aattcatttt attgacactg gaaatagtga tgcaatattg
     2041 attcaagatg gtaaaacttt tatattgata gatgggggag ataatgatga tgagggatta
     2101 atggtagatt acctaaacaa tcaaggtgta aaagatatta aatatcttat agctacccat
     2161 tctcatgcag accatttagg tggacttgat tctgttgtca aaaactttaa tatagaaaat
     2221 gtttttgtat cgaatggttc agcagaaaca aagagttata gggactttat aaatgcttta
     2281 gcaaataagg gtttatcccc aagtgtaccg cttgaaaata ataagttcta tttagaagat
     2341 tcttattttg aagttctaaa tacaaatggg ggagatacta ctaatgaaca atctctagtc
     2401 cttgtatata ctaatgggaa tgataaagtt ttatttacag gtgatgctga agaaggtacc
     2461 gaaaaagaaa tactacctaa gcttgagaaa gtagaccttt taaaagtagc tcatcatggt
     2521 tctcgtagta gttcatctca agagttttta gataaagtaa atcctgaata tgcggttctt
     2581 ttagtaggca agggtaattc atatggtcat ccacatcagg agacaatgaa taaattaaaa
     2641 aaaatgggag taaaagtaca tagaagtgat gagtgttctg atataatatt tgaatcaaca
     2701 ggtgatggag tatttacaag ttgtaaagat ggaagttata ataaaggcgt tagagaagat
     2761 ggaaactctg gaaaatcaaa cagtaataca agtaaaaatg attcttttaa aaataaacaa
     2821 aatacatcca attcagaaga aatagtgtat tttactccaa aaggtaagag ttatcactct
     2881 acaaaaaatt gtagtgggct ttcaagaagt aaaaaaatat tatcaggcac aattgcagaa
     2941 agtaagaaaa atgacccatg tgatatatgt tatggaaaat aacaaaaaaa caaccaattt
     3001 atctataaag cttctgaact ttaaatcatt taaaaaataa aataaatgca taaaaaaata
     3061 agctataatc aatagataac acattttatc tatacgaata gcttattttt ttaagtattt
     3121 tttaatagct atttaataac tctaaataca ccagttacaa tacctacaat ttcaacttct
     3181 gagtcattga gcataattgg ttccataaaa tcattttctg gctgaagtct tattaaattt
     3241 ccttctttaa aaaacctctt aactgtagca gattcaccat ttataagtgc aactactatc
     3301 tgagaattta aggctgtatt ttttttgtct actacaacgt aatcttcatc taaaattcct
     3361 gcattaatca tactttcgcc ttttactctt aatacaaaat tgttactgcc ctttactaaa
     3421 ctggcaggga atggaatgta ttcctctatg ttttgttgag ctaaaatggg ttctccagct
     3481 gttatttgac ctactaaagg aagctctatt atttcttgat tagctccaga aacatcattt
     3541 actttactac gttctaaaac ttcaatagct cttggtttag tagggtcctt tcttatgtat
     3601 cccagttttt caagtttatt tagatgagag tgcacagttg aagtagatct taaccctaca
     3661 gcagtacata tttctcttac agcaggtgga taacctttta atatgatttg agactttata
     3721 aattccaata tcaagacttg cttttcagtt agatctaaat acataacatc actcatttcc
     3781 aaacatattt tgtttttatt tccatacact ttatattttt aattataaca taatccattt
     3841 atatctgcaa acatttgttc ctgattttta atcgtcctca atccattcaa aattcttgac
     3901 tatatcacgt tttacatttt tcttatgagc tgcataaagc tgagtagttg tgacattatc
     3961 atggtcaagt aagttttgaa catcatatat agaaaatcct gtttggatta gagaagttgc
     4021 agccgttgct cttaatttat ggggactata tccattgtct ctagtagtat caagtggaat
     4081 agatgtatat ttttttacca gtgttcttat agcctttgct gtaattctct tattttgaag
     4141 agacaaaaat aaagcatctt tttcttcatc atttaatagt tcatctcttg ttctttcgtt
     4201 ttgcaaataa tcttttatta catgttcaca agtatgattt ataggcatta aaacttcttt
     4261 cccacgtttt ctatatattt taaattcacc tcttgaaaaa ttaaaagagg agatattgag
     4321 ttctctaagc tcatttaatc taagcccgta tgtaacaaat aatgctagta tagctttgtc
     4381 acgtaattta gtttttctcc aatatacctt ttctttctct gtaagccctt cacctgtttc
     4441 tactgattca agcatcttag caacttcatc tatctcaagg cgcttaatag catctggctg
     4501 aggtttagga agttttattg gattaaatcc atcagttata ttattatcta tctgagagtt
     4561 tctgtataag aatttaaata gagtagagat ggatgatttt tttcttgcta aagctctatt
     4621 gttattttca aaaatcaatg tgtttttttc tgtatttttg taatatctac tgcaataatc
     4681 acctaagaat aaatttatat ctcttgattt tattgtatta aaatcatctt ctgtaatatc
     4741 ttttacacaa tctgcatttg ttaattcttt ggtttcaatc aaatatgaac aaaaaaaatt
     4801 aatatcttct aaataagcaa gtcgtgtaga aactgcaaca gaacctttta aatatataaa
     4861 ataatccttc ataaatacag gaagtttatt ttccaaaagt agacatt
//
LOCUS       DAEMHW010000242         4797 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEMHW010000242 DAEMHW010000000
VERSION     DAEMHW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.243"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..932
                     /locus_tag="KPK67_003449"
     CDS             <1..932
                     /locus_tag="KPK67_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2460367.1"
                     /translation="KTFNSIYFVPLILDISFLIKEKYKYILFSFVIISSLIISLDKNI
                     YLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLRISKDELKKVNADLETYASSI
                     EELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLLKDKPLIYDLVHELREFVTES
                     FQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNINVKLTISKNTWNLSRNQSIA
                     LYRLIQESLSNSSRHGKATEIRIFITFNTSNLIITISDNGIGCGNIKKGNGLNSINER
                     IYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            925..1572
                     /locus_tag="KPK67_003450"
     CDS             925..1572
                     /locus_tag="KPK67_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2460368.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1701..2636
                     /locus_tag="KPK67_003451"
     CDS             1701..2636
                     /locus_tag="KPK67_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460369.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2650..3747
                     /locus_tag="KPK67_003452"
     CDS             2650..3747
                     /locus_tag="KPK67_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460370.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQNSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGNLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3750..>4797
                     /locus_tag="KPK67_003453"
     CDS             3750..>4797
                     /locus_tag="KPK67_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460371.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSLGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINASHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIVLI
                     LIGIIFVTAGSLKLR"
BASE COUNT         1870 a          548 c          657 g         1722 t
ORIGIN      
        1 caaaaacttt taattcgatt tactttgttc ctttaatatt agatatatca tttttaataa
       61 aggaaaaata taagtacata ttgttctctt ttgttataat atcttctctt attattagtt
      121 tagataaaaa tatatatttg gcactggagt caagctctat attgctaatt ataactgtac
      181 tttctatata tatagaaaat gagaatttat caaaattata cagtcaaaat ctttatgata
      241 agcttagaat ttctaaagat gaattaaaaa aagttaatgc tgatttggaa acttatgcaa
      301 gttctataga agaattagca attttaaaag aaagaaatcg aatatcaaga gaaatacatg
      361 atagtgtagg acattctctt tctacaacaa taatacaatt aaatgctata gaaaaactct
      421 taaaagacaa acctctaatt tatgacttag tacatgaact tagagaattt gtaactgaaa
      481 gttttcaaga tgtaagaagg gctatatctg agttaaaacc tgttgaatat gaaaattacc
      541 aaagtttatt taaaatcaaa gaattagtta aaagctttat taagcttaca aatattaatg
      601 ttaaattaac tatttcaaaa aatacttgga acttatccag aaatcaatct atagctttat
      661 atagactcat acaggaatcc ctttccaact catctaggca tggaaaggca actgaaatca
      721 gaatttttat tacattcaat acttcaaatc ttataattac tattagtgat aatggtatag
      781 gatgtggcaa tataaaaaaa ggaaatggac ttaatagtat caatgagaga atatatgaat
      841 taaatggtaa agttgaattt gataattcta ataatggttt cacaattaga gcttcttttc
      901 ctaaattttc agggggtgat ttttttgaat aaaattaaag ttcttatagt agatgacgaa
      961 aaattaatta gaaaaggtct taaaattata ttatcatctt ataatgattt agaaattgta
     1021 ggagatgctt ctaatggata tgaagcttta gaattttgta aaatgaatga tgtagacata
     1081 gttcttatgg atatacgaat gaaagtatgt gatggggttc taggtacaag gcttataaag
     1141 gaatataata attctataac attattaatt ttaacaactt ttaatgatga tgaatatata
     1201 aaagatgcta tgaagtttgg tgcttcagga taccttttaa aagatagctc tgatgaagta
     1261 ttgcatgaag ggattcgttc ttcatttttt ggcaatattg tgcttgataa aagtgtagct
     1321 gaaaaaatca tgacctctga aaaaacggtt aaacaagagc atctttatga catgtacaat
     1381 ttaacagaaa aagaaatatc tataattaga ctaattgcaa atggacttaa taataaagaa
     1441 ataagtcaag aactattctt atctgaaggt acaataaaga ataacataac taatatttta
     1501 gctaaactag aacttcgtga cagaactcaa ctagcaatct ttgctttcaa aaataaaatt
     1561 gtaatagaat aaatctaaaa atataaagtg acttaagtca ctttttttag tgacctatag
     1621 taatagaatt ataattcaca attctttatt ataatattat agctttttaa cttatacaaa
     1681 attattttgg agggattact atggatatag tcagagtaaa taatataaca aaaagattta
     1741 atgataaatt agtacttgat aacatcagct tttctgttaa aaaaggtgag atatttggac
     1801 ttattggccc aaatggtgca ggtaaatcta cacttataaa tataattaca aatcttatgt
     1861 taccaaatag tggtagtatt caaatcaatg gtttagattt atcaaaagat tatataaaag
     1921 caaagtctat aatagggtta gttccacaag aattagcaat tatagaaact cttactcctt
     1981 ttgataactt agagtacttt ggagctttct acggtttaaa gggaaaacta cttaaagaaa
     2041 gaatcattga agcattagaa gttacaggtc ttactgaagt caaaaagaaa aaggtcaaaa
     2101 aattatctgg tggtatgcaa aggagattaa atattggaat agcactctta aatcatccta
     2161 aaatattaat attggatgaa ccaactgtag gtgtagaccc ccaatcaaga aatcatattt
     2221 tcaattttat aaaagatata tctaaaaaac atgaaactac tgtaatatat acttcccatt
     2281 atatggaaga agttgaacac ctttgttcta aaatttttat tatggatgaa ggtaaagaaa
     2341 ttgcttttgg agataatgat tatttaaaat cactcgtatc tacaaataca aaattgatta
     2401 tggaaataaa aaatataaat gcccaactta tctttgattt aaaaaatact aaaggtgtta
     2461 tatctgtttt agaaaataat tcattattag aattggatat agataaaaaa ttacaactta
     2521 cagatatact ctctgttatt gacaaaaaca attcaaaaat aatgaaaatt tcttatgaag
     2581 aacctagcct agaagacgta tttttaaatc taacaggaaa aaatttaagg gactaatgga
     2641 ggtgatttta tgaaattata catgagtgta aaaactatgc taaagagttt aaaaagctcc
     2701 tttatactta atttaatata ttttttagct ttgcctctga ttttatcttg gtttcttggg
     2761 atggttacag aaagcatgtt tcaaaaccct ataaaaacag aatctacccc cattgttata
     2821 tatgacaaag ataacacaag attatcaaat gatttgacaa agtatttgaa aaatgattta
     2881 tcttatatat taacagttaa aaaagatgat tctaaggcag aattaaagct tactatacct
     2941 aaaggctatg agagtagttt attaaatgaa aaatcaaata ctctaaacat agaaaaactt
     3001 ggaactcgtg atgatattgc aatattgctt caagatatat tagatactta ccatgaaaaa
     3061 ttttatttaa ataattctca gaaaataagt tcagaagatt tttctaaatt atttaataaa
     3121 aattctattg atacttctat aataaataac aatgttaaac agaactctta tgaatacttt
     3181 gctttagttt cactaggatt tcttgtaata atttttataa tgaacaatat tttgtcaaat
     3241 tatataagtg aatcaaaggg actttcaaaa agactttatt ctatgcctat aactagagta
     3301 caattcttaa tttatgactt tgtaggttta tggatttatt catttatatt cttattatta
     3361 tatgttttat tctttagaat acttggaata acatttaaag gtaattttgc catattgctc
     3421 ctactttgtg ctttatcatc ttattttatg acttcaatat ctacctttgt aaccagcttt
     3481 tttagtaaaa aatacggtac agcaatagtt tatgcattac tctttttaca gacaattttt
     3541 ggaggtatct tcagtatgat aagtgataca tttactaagc ttacaagttt atctcctacc
     3601 tatttaatag gagaattgtt tagtaattat gaaacattta aaactataga ttcaatagga
     3661 aatttaattt tcacatgttt aattatctct acaatattaa ttgcacttgc tattgtaaag
     3721 gaaaaatata aatggaggga ggtttaataa tgattagatt aactattgca aatataaaaa
     3781 gatatctaaa aaatcatact cttttaatta atatggttat gcttcctatt atactaattt
     3841 tttcacttaa tttttttatc aacaactcgg gaaatcaatc aatgtacttt tctccagtag
     3901 caattgtatc tgactcttta ggaaagtatg aacataagtt aataaatagt tctaaattaa
     3961 aagaaaattc atttagatta aatgaacaag ataaggctat gaatctatta aaaaataata
     4021 aggtttctgc tgtattcgtt ttagataaaa atttttcaaa tagtatagat aaattacaac
     4081 gacctgtagt taaatgcttt aaaattgaaa atggtggtgg tagtctttgg gcagaatcac
     4141 aaatagaatc ttttattact aaatcgttaa aattgaaagt ggataaaaat atagatgata
     4201 aattgacaaa gacaaatata attgataaca aaccagataa gaataaaggt tcatttttag
     4261 tagtcttctt gatatgctat tttatgtata ttaatgcgtc acatcttgct tctgatttat
     4321 ttacattaaa aaaatcaaat gtgctaaaaa gacttatctc tacagataat aaagatataa
     4381 aaataatttt cagtatattt ttaggactat tttttattca atcaattgta tacacttttg
     4441 ctctattatg tttttcaatt atagaagact ttaatttgag tctgaatgta ttgatgattg
     4501 tactagcaaa tagttttgtt tccactggat ttgtattttg ggtagctaga gtatttaaaa
     4561 atgaaagttc tataagtctt atatctacat tttattcttt aataggtcta gctatatcta
     4621 tctctagtct aataccatca atggataaat tatcctttat gactaatctt tcaaagttca
     4681 caccatttta ttggactata gatgctatta aaaacaatgg tagtatttta ataaatataa
     4741 tagttctaat attaattgga attatatttg taactgcagg aagtcttaaa ctaagag
//
LOCUS       DAEMHW010000243         4750 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEMHW010000243 DAEMHW010000000
VERSION     DAEMHW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.244"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            13..1080
                     /locus_tag="KPK67_003454"
     CDS             13..1080
                     /locus_tag="KPK67_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBG2460372.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKDSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            1250..3151
                     /gene="cooS"
                     /locus_tag="KPK67_003455"
     CDS             1250..3151
                     /gene="cooS"
                     /locus_tag="KPK67_003455"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG2460373.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3144..3617
                     /locus_tag="KPK67_003456"
     CDS             3144..3617
                     /locus_tag="KPK67_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2460374.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3592..>4750
                     /locus_tag="KPK67_003457"
     CDS             3592..>4750
                     /locus_tag="KPK67_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG2460375.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENR"
BASE COUNT         1887 a          500 c          944 g         1419 t
ORIGIN      
        1 aggagattta ctatgaaaaa taaattagtt tacatatgta ttatgagtct attgctattt
       61 gtagtaggat gttctaataa tccaaataag gaaagtacca ttaaaaaaga ctctaagaat
      121 aaccaaagcc aagttaaaga agaaaaaaga gaacctgtga aaaaagatat aactataagt
      181 ttcgctggtg atgtaactat ggggaactat aaagattcat catattatgg cagttttgac
      241 catgaattta aaagacaagg agaagattac aattattttc ttaaaaatgt aaaacctgta
      301 tttgaaaaag atgacttaac agtggttaat ttagaaggtc cacttactac agcgtcaaat
      361 gcaaaagaaa aaaaatttgc atttaaagga aagcctggat atgtaaatat tttaaaatct
      421 ggaagtgtag aggcagtaag tattgcaaat aatcattcag aagattattt tgaagaagga
      481 atgaaagata cacagtttat attggacgag aataaaataa attattttgg attagggaaa
      541 gatgctgttg tagatgtcaa aggaattaaa gtgggtctat tagggtataa tggattatca
      601 actgaatata atgaaaaaaa tttaaaacaa atggaagatg atattaagtc cttaaaaaag
      661 aaatcggatg ttgttgtagt gtactttcat tgggggatag aattagatta ttatccagat
      721 aaaaaacaaa aagattttgc tcattatgct atagacaagg gagcagattt agttatgggg
      781 tctcacccgc atgtgattca aggaatagaa aagtataaaa ataagtatat agcttatagt
      841 ctagggaatt tttgctttgg aggaaataag aatccttctg atacagatag ttatatatat
      901 caacaaacct ttactttttc agataataaa ttaacttcaa ttaaagagcc aaatataata
      961 ccaacttcaa taacatctag taactccaga aataattatc aaccaaaagt attggatgga
     1021 agtgagaagg atagagtttt aaataaactt gaaaaaataa gcaaagattt aaataaataa
     1081 aatgtaaaaa aaaataaata tatatattta ttttaataat ttatattttg tttagggtat
     1141 ggtaaatcgt ggtattaaat aagtagattt taatagttaa attatagtca attataataa
     1201 agctatttaa tctacttttt taatataaaa atataagggg gaatttgtaa tggcttgtaa
     1261 cacttgtaaa atgtgcgaaa gtgcagacaa gaaattagaa agttttgtag cttcaaagga
     1321 tgttgaaaca gcatttcata gaacagaaga tcagaaagtt aagtgtggtt ttggacttca
     1381 gggggtttgt tgtagactct gttcaaatgg accatgtaga gtaactccta aatcaccaag
     1441 aggtatatgt ggagcagatg cagacactat agtagcaaga aactttttaa gagcagtagc
     1501 atcaggagca gcttgttatt tacatgtggt agaaaataca gctaagaatt taaaaaatgt
     1561 tggaataact aaaggtgttg ttaaaggaga aaaaacatta aatcaattag cagagatgtt
     1621 tggaatagaa agtgatgaaa agtatgataa atgtataaag gttgcagaca aagttatcaa
     1681 cgatttgtat aaatcaagag atgataaaat ggaattagta gaaaaaatag cttatgcacc
     1741 tagagtgaaa aagtggaaag agttaggtat aatgcctggt ggtgcaaagt cagaagtttt
     1801 tgatgcaata gtaaaatcat caacaaattt aaatagtgac ccagtagaca tgctagttaa
     1861 ttgtttgaac ttaggtatat caacaggatt atatggtctt acactaacta atcttttaaa
     1921 tgatgttatg ttgggagaac cagttataag aatggctcct gtaggattta atgtaataga
     1981 cccagactac ataaatataa tgataacagg ccatcaacat tcaacatttg caaatttcca
     2041 agatagatta aaagatgaag atgttataaa attagctcaa tctgttggtg caaaaggctt
     2101 taagctagta ggatgtacat gtgttggtca agatttacaa ttaagaggag aacattatca
     2161 agaagtattt gctggacatg caggtaacaa ttttacaagt gaggctgttt tatcaactgg
     2221 agcaatagat atagttcttt cagaatttaa ctgtactata ccagggcttg aaccaatagc
     2281 agataaatat aaagtaaaaa tggtttgttt agatgatgtt gctaaaaaag cgaatgcaga
     2341 atacataggt ttagacagaa gtaaattaga tgaacttagt aacactttaa tagaaaaagc
     2401 attggagtct tataaggaga gaagaggaag tattgaaata gatataccaa aagaccatgg
     2461 atttgaacaa tctttaactg gtgtaagtga aaagaacctg aaagcgtttt taggagattc
     2521 atggaaacct cttattaatt taattgcaga aggaaaaatt aaaggtgttg cagctgttgt
     2581 aggatgttca aatatgacag caggaggaca tgatgtaaat actgttgaac ttactaaaga
     2641 attgattaaa aaagatataa tagtactatc tgcaggatgt tctactggag gattagaaaa
     2701 tgttggtctt atgtcacctg gagcagaaga attagcagga gaaaacttaa aagaagtatg
     2761 taagacttta ggaataccac cagtattaaa ctttggacca tgtctagcta taggaagact
     2821 agaaatagta gcaacagaat tagcagctga acttggaatt gacctaccac aattaccatt
     2881 agtgctttca gctccacaat ggttggaaga acaagcatta gctgatggag cgtttggtct
     2941 tgcccttggc ttacctcttc atttagcttt acctccattt ataacaggag gaaaattggt
     3001 gacagaagta ttaacagaaa aattaaaaga cttaacagga ggacatgtta tagtgaatcc
     3061 agaccctaaa tcttcagcta atcaattaga agaaataata atagatagaa gaaaaaattt
     3121 agggttaaag gatgtggaat gcaatgcata gaatttttat aaataaagat ttatgtacag
     3181 gatgtaagag ctgtgtacta gcatgtatgc taaaacacaa taaagattat gacatgtaca
     3241 ctcttgattt agaaaatata gataatgata gtagagggca tatagaatta gatagtaaac
     3301 ataataatcc agttccaatt ctttgtagac actgtgatga acctgaatgt gtattagcat
     3361 gtatgagtgg tgcaatgcat aaagatagtg aaagtggaat cgtttcatat gatgaagaga
     3421 aatgtggttc atgttatatg tgtgtaatgt cttgtccata tggattatta aagccagatg
     3481 atagaagcaa gcagaacata cttaaatgtg atttatgtaa agatgaggag tatcctagat
     3541 gtgtagctaa ttgcccaagt ggagcaattg aattacagaa ggaggaaaat gatgaattat
     3601 gtagtgttag gggctagtgc agctggtata aatgcagtaa aaacattaag agaattagat
     3661 aaagattcaa atatagtagt aatttctaaa gatgaaaacg tatattcaag atgtatgttg
     3721 catcacgtta tatctgaaca cagaacttta aaacaaataa attttgttga tgaagatttt
     3781 atggagcaaa ataatgtaaa atggattgca ggtaagactg ttaaaggtat agacataaat
     3841 aaaaaggtag ttcagacaga agacattact gtaaattatg ataaactttt aatagctaca
     3901 ggtgcatctt ctgctattcc tcctataaaa aatttaagag atggaaattt tgtatattct
     3961 gtgagaaaca tagatgatat ttataaaata aaagaaaaag cacaaaattc taaaaatgta
     4021 gttataatag gtgctggttt agttggtata gatgcattag tgggattatt taaatatgag
     4081 cagttaaata tttctgtagc ttttatggaa aagtacattt tagatagaca attggatgaa
     4141 tatacagcat cagtgtatga aaataagttc aaagaaaaag gtgtaaaatt ttacccaagt
     4201 gcatcaatac aagaaattgt attggataac agtaaaaatg ttacaggtgt tgcattttca
     4261 aatggtgaag ttttagatgc agacatggta attgtagcta caggggttaa accaaatgct
     4321 gactttttag atggaacagg aatagaatat gatagaggta taataataga tgatatgtgc
     4381 caaacaacac aaaaagacat atatgcagca ggagatgttg taggtaaaaa tgctatttgg
     4441 cctctggcag taaagcaagg aattgtagct gcctataata tggttggaaa agataagaag
     4501 atagaagatg aatttgcttt taaaaatagt atgaatttca tggatatacc aactatttct
     4561 ataggaatga atacacctgt agatgatagc tataaagtat taaccagaca tggaattaat
     4621 gattataaaa agtttgtttt taaagataat gtaatctatg gagcagttat acaaggagat
     4681 atttcatatg taggtgtatt aacttattta ataaaaaata aagttgaaat atatgattta
     4741 gaaaatagga
//
LOCUS       DAEMHW010000244         4745 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEMHW010000244 DAEMHW010000000
VERSION     DAEMHW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.245"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..513
                     /locus_tag="KPK67_003458"
     CDS             <1..513
                     /locus_tag="KPK67_003458"
                     /inference="COORDINATES: protein motif:HMM:NF025854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2460376.1"
                     /translation="IKKIENGDVDNYIKSINEELRDFESSFNTGNKILDIILNEKSID
                     CRNNNIKLLCDINLRKCEFIQMIDISSIFSNILDNAIEACEKVRQGRYIKLRGVIVKS
                     YFIITCENSKNDKLRFKNNILITSKDDKFFHGIGLKSIKSSIKKYDGELEIIEGKDNF
                     TLNIYIPIQN"
     gene            673..1257
                     /locus_tag="KPK67_003459"
     CDS             673..1257
                     /locus_tag="KPK67_003459"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460377.1"
                     /translation="MKKFKLYGNCINKNIKLIESKKKILLILFVVSLLLCVCSFFIKS
                     LITGAPRDNLIRIYNILGTSFDRMIEQENFKNKDVYKEELSKLLNTENFKNVSKIEVY
                     LNQNPKNHNKKSMNLMYSYENRLKKNTWEAFISSKLRTYDGKEIYCHIVCEEPKYEAY
                     KLPVNIICILSLVVASIFLILYLYMIVKFHKIGR"
     gene            1262..3901
                     /locus_tag="KPK67_003460"
     CDS             1262..3901
                     /locus_tag="KPK67_003460"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG2460378.1"
                     /translation="MKMIFVLLRGYAIKNRKKFLIMIFAISVSTTIIFGTTIARLSQS
                     KYVSDEIYKQSPSYQIEVGNISEEDFETIIKDNNVKSSVLRKYYGNFSYNEKFYVLEE
                     YNKVSFKKLKHTLNNGRFPKQKDEIMISNNLFKELKSQNKLKLIDTHSHKKEYEVELS
                     YSKEYIDNLKEHQIFNQTHKFKIVGTYNMTSIMENSSEGIGIYVYKDFEYPEQLTTYN
                     GFIDLKTGFKNVQTEIDKLSIKLDSGELNIKSNKTLATAKEDSQSALGGFDLFDTGTI
                     VASICIIFNVFNIMMKELVQEIGLLRVVGMSKRQSLFIFMLKNILVLLVGSLIGFLGG
                     YLLAEVMIKYFNLTSGFIDVSDAPIYVSKSAVFKTLKLTISMLLISTILPILITLKSY
                     PVNMMSGRLRNPINIFDEIFNKIKIYGKLKILGLNFLKKIRNLSKLERKLFFRDNVDW
                     NIALNNSKRNSIYILTTAIIVGMAGLYGVRQFITSQQVSSIGDPLLQRLGDYSIDLSY
                     NGISKLDDSLISQQDLDNISSIDGVSDVYKFNSTTAYAKIKTKDLSSAYRKNLSIKDD
                     NREIEMKFDFIGLNKDALSKYDSFLDSGQLYKKNNNVIEAVVSNYFLDQTYTHSQQKY
                     SEKLKIGDTLKFKVLSEENGVKKYNNIEIKIVGFLSEEWFTKGTYTNSQTPDIIVDSS
                     DYKKITGDNNFSQVKIKVEEQDLENIKLSIENILKDKKYIKYSDKNTINQEQGSLAWQ
                     TIIRNIVNSAMLSITALTNIVFSVVTSITMRKKELGLMRAIGLSIKDLKRILMYEGLI
                     YGVISSTVGVLFILYKGISWVHLLKMTAKFQGIPYEGPLFIFPVIPTLVFILSTIIIS
                     LISVTFTFKIINKYSIVNQIREN"
     gene            3929..4594
                     /locus_tag="KPK67_003461"
     CDS             3929..4594
                     /locus_tag="KPK67_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460379.1"
                     /translation="MRNLSMKGIRKIYGENENKVNALNGIDLEINPHKFTAIIGASGS
                     GKSTLLHCMAGLDKPTSGHIYLDDIDIYTMDDTKLSRIRREEFGFIFQSFNLIPVIKV
                     YENIIMPVSLGCEKIDKKYIDMLIDKLGLREHINKYPNQLSGGQQQRVAIARALANKP
                     SVIFADEPTGNLDSETTIEVMDLLKTCVKEFKQTLVMITHNNDIASMADKIITIKDGM
                     IYE"
BASE COUNT         2013 a          410 c          757 g         1565 t
ORIGIN      
        1 atcaaaaaaa ttgaaaatgg agatgtagat aactatataa aaagtataaa tgaagaatta
       61 agagattttg aaagtagttt taatacagga aataaaatac ttgatattat tttgaatgag
      121 aaaagtatag actgtagaaa taataatata aagttattat gtgatataaa cttaagaaaa
      181 tgtgaattta tacagatgat agatatatca tctatatttt ccaatatatt agataatgca
      241 atagaagcat gtgaaaaagt aagacagggt agatatataa aattacgtgg agtcatagta
      301 aaatcttatt ttatcattac atgtgaaaat tccaaaaacg ataaattaag atttaaaaat
      361 aatattctaa ttactagtaa agatgataag ttttttcatg gtataggttt aaaaagtata
      421 aagtcatcta taaagaaata tgatggagaa ttagaaataa ttgaaggaaa ggataatttt
      481 acattgaata tatacatacc aatacaaaac tagttaaact acacttcaaa tatgatagtt
      541 atgacagtat aattaccact tgggcaaata tattgagtaa aaaaaataaa ggtgttatct
      601 tttaaacata aaagataaca cttttatttt tttgattatt ttaaataaat aattttaagg
      661 agaatgtttg gaatgaaaaa atttaaatta tacggtaatt gtattaataa aaatataaaa
      721 ttaattgaaa gtaaaaagaa aatactttta attttgtttg tggtatcttt acttctatgt
      781 gtatgttctt tttttattaa atctcttatt acgggagcac caagggataa tcttattaga
      841 atatataaca ttttaggaac ctcatttgat aggatgatag agcaggagaa ttttaaaaat
      901 aaagatgtat ataaagaaga gttatctaaa ttattaaata cagaaaattt taaaaatgta
      961 agtaaaatag aagtatatct taatcaaaat ccaaagaatc acaataaaaa gagtatgaat
     1021 ttaatgtact cttatgaaaa tagattaaaa aagaatacat gggaagcatt tatttcaagt
     1081 aagttaagaa cttatgatgg aaaagaaatt tattgccata tagtatgtga agaaccaaaa
     1141 tatgaagcat ataaattacc agttaatata atctgtattc tttctttagt agtagcaagt
     1201 atattcttaa tattatattt atatatgatt gttaagtttc ataaaatagg gaggtaagaa
     1261 tatgaaaatg atatttgtat tattgagagg atatgccata aaaaatagaa aaaagttttt
     1321 aataatgata tttgctatat cggtttctac tactataatt tttggaacta caattgcaag
     1381 gttaagccaa tcaaaatatg tttccgatga aatttacaaa caaagtccaa gttaccagat
     1441 tgaagttggc aatataagtg aagaagattt tgaaacaata ataaaagata ataatgtaaa
     1501 aagttcagta ttaagaaaat attatggaaa ttttagttat aatgaaaaat tttatgtatt
     1561 ggaagagtat aataaagttt cttttaaaaa attaaaacat actttaaata atggtagatt
     1621 tccaaaacaa aaagatgaga ttatgattag taataattta tttaaagaac taaaatcaca
     1681 aaataaattg aaattaattg atacacattc gcataaaaaa gaatacgagg ttgaattaag
     1741 ttactcaaaa gaatatatag ataatttaaa agaacaccaa atatttaatc aaactcataa
     1801 atttaaaata gttggaactt acaatatgac aagtattatg gaaaattcat cagaagggat
     1861 agggatatat gtgtataaag attttgaata tcctgagcag ttgactacat ataatggatt
     1921 tattgattta aaaactggtt ttaagaatgt acaaactgaa attgataaat tatctattaa
     1981 gcttgattca ggagaattaa atataaagag taataagaca ctagctacag caaaagaaga
     2041 tagtcaatct gctttgggag gatttgattt atttgataca ggcactatag tggcatctat
     2101 ttgtatcata tttaatgtat ttaacattat gatgaaagaa ctagttcaag aaataggatt
     2161 attaagagtt gttggtatga gtaagaggca atctttattt atatttatgc tgaagaatat
     2221 attagtgtta ttagttggat cattgatagg atttttagga ggttatttac ttgcagaagt
     2281 gatgataaag tattttaatt taacaagtgg atttattgat gtttcagatg cacctattta
     2341 tgtgagtaaa tccgcagtat ttaaaactct taaattgaca atatctatgt tattaatatc
     2401 aacaatatta ccaattttaa taactttaaa atcttatcca gtaaatatga tgtctggtag
     2461 actaagaaat ccaattaata tttttgatga aatttttaat aaaataaaga tttatggaaa
     2521 gttaaagatt ttaggcttga attttttaaa aaagataaga aatttatcta aattagaaag
     2581 aaaattattt tttagagata atgtagattg gaatatagca ttaaacaatt caaaaagaaa
     2641 tagtatctat atattaacta cagcaattat tgttggtatg gctggacttt atggagtgag
     2701 gcagtttata acatctcaac aagtatcaag cataggtgat ccattacttc aaaggttagg
     2761 agattacagt atagatttat cttataatgg tattagtaaa ttagatgatt ctcttatctc
     2821 acaacaagat ttagataata tatcttctat tgatggagtt tcagatgttt ataaatttaa
     2881 ttcaacaact gcttatgcta aaattaaaac aaaagattta tcttcagcat atagaaagaa
     2941 tttatctata aaggatgata atagagaaat agaaatgaag ttcgacttta taggattaaa
     3001 taaagacgct ttatctaaat atgatagttt tttagatagt gggcagctat ataaaaaaaa
     3061 taataatgta attgaggctg tagtaagtaa ctatttttta gatcaaacct atactcatag
     3121 tcaacaaaag tatagtgaaa aattgaaaat aggtgataca ttaaaattta aagttctatc
     3181 agaagaaaat ggagtgaaaa aatataacaa tatagaaata aagattgttg gatttttaag
     3241 tgaagagtgg tttacaaaag gaacatatac taactctcaa actccagata taattgtaga
     3301 ttcaagtgat tataagaaaa taactggaga taataacttt tctcaagtaa aaataaaggt
     3361 agaagaacaa gatttagaaa atataaaatt atctattgag aatatactta aagataaaaa
     3421 atatataaag tatagcgata aaaatactat aaatcaagaa caagggagtt tagcatggca
     3481 aactataatt agaaacatag ttaattcagc tatgcttagt ataactgcat taacaaatat
     3541 agtttttagt gtagtgacta gtataactat gaggaagaaa gaattaggat tgatgagagc
     3601 gataggttta tctataaagg atttaaagag gatattaatg tatgaaggtt tgatatatgg
     3661 tgtaataagt tccacagtag gtgtattatt tatactttat aaaggcatta gttgggtaca
     3721 tttactaaaa atgactgcaa agtttcaagg tataccatat gagggcccac tatttatatt
     3781 tccagtaata ccaacactag tatttatatt aagtacaatt attatatctt taatttcagt
     3841 aacatttaca tttaaaatta ttaataaata tagcatagtt aatcaaataa gagaaaatta
     3901 agtttaacat aagaaggaga aaaattgtat gagaaatttg agcatgaaag gtataagaaa
     3961 aatatatgga gagaacgaaa ataaggttaa tgctcttaat ggcatagatt tagaaataaa
     4021 tccacataaa ttcacagcaa ttataggggc tagtggctca ggtaaatcta cattacttca
     4081 ttgtatggca gggttagata agcctacctc aggtcatata tatttagatg atatagatat
     4141 atataccatg gatgatacga agctatctag gataaggaga gaggaatttg gatttatatt
     4201 tcaaagtttt aacttgattc ctgtaattaa ggtatatgaa aatattataa tgcctgtgtc
     4261 attgggttgt gaaaaaatag ataaaaaata tattgatatg ttaatagata aactaggatt
     4321 aagggaacat ataaataagt atccaaatca attgtctggt ggtcagcaac aaagagttgc
     4381 tatcgctcga gcattagcaa ataaaccttc agtaatattt gcagatgagc caacaggaaa
     4441 tttagatagt gaaacaacta tagaagttat ggatttactg aaaacttgtg taaaggaatt
     4501 taaacaaact ttagtaatga ttacacacaa taatgatata gcatctatgg cagataagat
     4561 tataaccata aaagatggga tgatatacga ataattttgt aattaaaaat attaagaatg
     4621 gggtgcagtt aaagaggaaa attatttgaa caattgatga aagaacatcc agagtttttt
     4681 acagaggaat aaattgagat aaaaaatagt tttaacaatg aaagcaaaaa aggtattgat
     4741 gaaga
//
LOCUS       DAEMHW010000245         4745 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEMHW010000245 DAEMHW010000000
VERSION     DAEMHW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.246"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            190..1341
                     /locus_tag="KPK67_003462"
     CDS             190..1341
                     /locus_tag="KPK67_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBG2460380.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1387..2562
                     /gene="thiI"
                     /locus_tag="KPK67_003463"
     CDS             1387..2562
                     /gene="thiI"
                     /locus_tag="KPK67_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBG2460381.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2718..3521
                     /locus_tag="KPK67_003464"
     CDS             2718..3521
                     /locus_tag="KPK67_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG2460382.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDVHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3764..4405
                     /locus_tag="KPK67_003465"
     CDS             3764..4405
                     /locus_tag="KPK67_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG2460383.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1900 a          452 c          920 g         1473 t
ORIGIN      
        1 taggatatag tataagtata ttatatttta ctataaaaat ttgtaataaa gtgaaaaaac
       61 tagcttttca tgaaataata ttttataatt taagattgtc tttttaataa agattaacaa
      121 aaaaaatact ataattagtg gtataatagt tagagttgaa gattttaaat aaaggtaggt
      181 gtaattaagt tggaaatata cttagacaat agcgcaacta caaaacctta tcaagaagtt
      241 atagataaaa tggtttatgc acttaatacg gaatatggaa atccttcttc tgtccataga
      301 aaaggtgtag aagttgaaaa agctataaaa gaagtaagac aagatatagc taaatctcta
      361 ggagcgaaag aaaaggagat ttattttaca tcaggtggta cagagtgtaa taatactata
      421 ataagaggta ttactagttt aaataaaaaa agaaaaaatc acataatatc tactaacata
      481 gaacatcctt ctgttttaaa tacattaaaa gatttagaag aagatggttt tgaagtaact
      541 tatttagagg tcggaaaaga tgggaaaata aatatagagg atttaaaaaa tgcgataaag
      601 tcaactacat gcttagtaag catgatgcat gttaacaatg aaataggaac tatacagcca
      661 ataggtgaag ttggaaagta tttgaagggt ttaaaagaaa aaatttattt ccatgttgat
      721 gctattcagt catatggaaa aattaacttt agaccatcta aatacaatat agattttatg
      781 agtgtaagtg cacataaatt tcatggtcca aagggaattg gatttatgta tataaaagaa
      841 aataatcgcc ttaagcctat gttaacaggt ggtggtcagg aaattggaat taggtcggga
      901 acagaaaatg tacctggaat atatggtctt ggagaagctg ttagaatact aaacaaagat
      961 ttagatgttg ttatttcgaa agtagataac ttaaaaaata tattaaaaaa tgaaataatt
     1021 gataatatag aagatataaa aatcaattct ccagaagatg gagtatgtca tatattaaat
     1081 gtatcattta gagggacaaa aggagaagtt ttacttcatt acttagagca aaaaggtatt
     1141 tatgtatcaa ctggctctgc atgttcttct aaaaagaaag gtagttacgt attaaatgct
     1201 ataggtctta caaatgaaga aataaatgga acaataagat ttagtttatc tgatatgaat
     1261 acagaagaag aaatgttaga agctgtgaaa gttttgaaag aatcaatttg tgacttaagg
     1321 tcaataatga aaagaaaata aagaatactg tataaatagt gataataaat tagtttaaga
     1381 ggtgaaatgg tgtataatat attaatagta aagtatggag aaattggagt aaaaggaaag
     1441 aatagatata tatttgagaa taggcttata agaaatataa gaaatatgtt aaaaccaatt
     1501 ggaagattta atgtatataa agagtatgga agaatatatg ttgatttaga agactatgat
     1561 tatgaagaag taattgaaga agttagaaaa gtatttggga tagtgggtgt gtgccctgga
     1621 gtaagagcta aaaaagatta tgatacatta aaagaaatag ctttaaaaat gttagaagaa
     1681 aaaatagagg ctggatataa aacttttaag gtagagtcaa gaagaggaga taagtctttc
     1741 gggttgactt ctcaagaaat gagtatggat ataggtggat acttattatc aaaggtagga
     1801 gataggatta atgttgatgt aagaaatcca gaagttaaaa taaagtgcga atacagagaa
     1861 tttcatacta tggtctatag tgatactata cctggatatg gtggattgcc acttggaacg
     1921 aatggtagag ctatgtctct tttatcagga ggtatagata gtccagtagc tacatggatg
     1981 gttgcaaaaa gaggtatgga agtagaggca gttcattttc atagttatcc atttacaagt
     2041 gaaaggtcac aggaaaaagt taaggattta gctaaaatac tggcaaaata ctgtggtaga
     2101 gtaaggcttc ataaagttaa tatcttagaa atacaaaaag caattggcga gaattgtaat
     2161 gaagaagaag ctactattct ttcaagaaga tttatgatga ggatagcaca gagactttct
     2221 gaaaaaagac attgtgatgc tttaattaca ggtgaaagta ttgggcaggt tgcatctcaa
     2281 actatacaag gacttacttg tactaatgcc gttgtagact taccagtgtt tagaccactt
     2341 atagcaatgg ataaatcaga tatagttgat atagccaaaa aaatagggac ttttgaaaca
     2401 tctatagtgc ctgaagaaga ttgttgtagt gtattttctc ctagaaagcc cgtcactaaa
     2461 cctagattag agaaaataga aaaatctgaa acagcacttg acattgaaaa attagtacag
     2521 gatgctattg acaaaataga ggtagaggat attgagtttt agcatacaag ttatttaatt
     2581 actaaattga atattgagta tattagtagt tctgtaagta tattagtagt tctgttgaaa
     2641 ggatttatgt agacaattta ttaatatgta gtatttaaaa ataagtacta taattttatt
     2701 taatgggaat gattattatg aataaaaagg gttttacact aattgaattg ttagtagtta
     2761 tatctataat aggcatttta gttatagtag ctgttccagc attgtttaga aatatagaaa
     2821 aaagtaaagc agttacatgt ctttctaata gagaaaatat aaagactcaa attgttattg
     2881 caatggctga ggaatcaagt aaaggcaaga atgaagtcat gaaagaggta ttagaaaaca
     2941 aagatggtaa gtactttgaa acagaaccaa agtgtaagtc aggtggaata tattcagcaa
     3001 cgtttgatga tggttatgat ggaataactg gaatagaaag cattgcaaaa gtgtatgtta
     3061 cttgtacaaa acatccagac ggtgttgaaa tggctaggga tgtgcatcaa agtatgaaag
     3121 atttgattgc atcatttgca caagaccctt ctataatacc aggagcttca aagggcaatg
     3181 atgattttag aaaatattta ttagacaata aatataaaaa tgggtggcct acaattccag
     3241 atgaatttaa ggcaaaatat ggattaagta aggatacact atatatacaa ccatatgcat
     3301 ataatcctac taaatctgat gctactgtag ttgtatttgc aaataataag actggaggta
     3361 attggtatac ttccctagtt tacgattatg atgaaggtag atggtataaa ggtaaaaatg
     3421 gtatttctgt tgcaggtagg tcatgggatg ttgacacaga tagtgttaag tctgtaaaaa
     3481 cagagattca ttctaaagag ggatggggtc ctttaaatta atatatcatg ttttattaat
     3541 ctggtattga ttaaactata gaaaagagca gtttctatag atgacgaata ccagattttc
     3601 atttatatat ataattgaga tatattaaaa gtgtttatat ttttaagtct tcacatacaa
     3661 gctaaaaata attataataa tattgtaaag aaatttaatt ataagattaa ttttctgaag
     3721 atttagtatt tgtactaaga aatacataaa aaggggaata gatatgaata ataattttca
     3781 agacaaccaa acaataaatt taatacaaag tcgtagaagt atacgaaaat ttacgacaga
     3841 acaaattagt gatgagcaag taaacacatt attacactgt gcatttgcag caccttcagg
     3901 atgtaataaa cagccttggc acataactgt tgtgcaagac cagaaacttt taaaagagat
     3961 tagtgatgat actttatcta ggattcatga agtgagtaat gttgagataa ataaaaactt
     4021 taagttattt tatggtgctc caaccgtttt atttatttct tatgatgaaa gtagttcatg
     4081 ggctccatat gatataggaa tacttacagg gaatataaca acagctgcac aagcactagg
     4141 gcttggaagt tgtataatag gaatggttag aggtttattt acacctgttg aacaagggga
     4201 tattgaaggt cttgtgagtg tattggataa agaagatgta aaagaatcag agtcaataaa
     4261 gatgaagttt gatacaaata aaaaatacag agaactactt gatataccag aagggtattc
     4321 tgtaccattt ggtattgctg taggtattcc agatggaaat ttaccaaatg ctagagaggt
     4381 agtttacaaa gtttccagag tttaggataa agtattgcaa gaaccaatca gttacttgaa
     4441 gaattaaacc agtaatatac aaataaattg atggaagagg aagctattta aaaagtagtt
     4501 tagatttttc gttgtaaaat aattaaataa agaataaggt gataagtatt ttatttatta
     4561 tcttattctt ttttattttt gttttgtaga atgataaaaa taaggattaa gtgtaataaa
     4621 atagaaaaca agtatgttaa ttttaagtat atgctttaaa atttatccaa gttaaaaatt
     4681 taagatatct tttcagtata atggaaaaaa tgtcaaaata ttgaaatagg gaaaaaatag
     4741 tgata
//
LOCUS       DAEMHW010000246         4727 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEMHW010000246 DAEMHW010000000
VERSION     DAEMHW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.247"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            396..1562
                     /locus_tag="KPK67_003466"
     CDS             396..1562
                     /locus_tag="KPK67_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBG2460384.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFVWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIKEN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            1584..3230
                     /locus_tag="KPK67_003467"
     CDS             1584..3230
                     /locus_tag="KPK67_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBG2460385.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            3327..4700
                     /locus_tag="KPK67_003468"
     CDS             3327..4700
                     /locus_tag="KPK67_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2460386.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
BASE COUNT         1763 a          537 c          891 g         1536 t
ORIGIN      
        1 ttggataggt tctatcctat ctctacatat aggtttccct atatgctgac tattttaact
       61 actaactcat gactaaagtc acgagtgtgc gtagctattt tttaatcaaa tagtttgaaa
      121 ttaacatcta aattatcaat aggttcaatt atttcatgtg aaaaattatt tctgttcata
      181 ataatatcct ccttataatt ttaaattata aatagtaaat ttataatgtt caatattaaa
      241 aagataatat agactaataa attagataat aaacatactt aagaaaacgg ataaaatcaa
      301 atataataac ggtatattaa aataacttaa aaaaattatt ttattataag tgcaaaatat
      361 acctatttaa ataagataag aggggaggat tttatgtgta tataaaaggt gtaaatttag
      421 gaggttggtt agttttagaa aaatggatga cctcaagttt atttgaaggc acagaagctg
      481 aagatgaata ctatttacca agacagcttt caagagaggc atatgaatct agaattaaga
      541 ctcatcgtag tgaatacatc acagagagag attttgcaac gattaaatct atgggattta
      601 attcagttcg tataccagta ccatacttta tttttggaga ttgtgaacca ttcataggat
      661 gtgttaaaga acttgataaa gcatttgttt gggcagataa gtatgggctt agtatattga
      721 tagacttaca tacagttcca ggaagccaaa atggatttga taatggagga atatcaggta
      781 tatgtagttg gtcacaaaat ccagaatatg tagcgtttac attaaatgta ttagaaagac
      841 ttgcaaaacg ttatgggatg aggcatgaat tgtatggaat ccaaatttta aatgagccta
      901 taacagaaag aatgtggaac ataatgaatg ttccaaatcg gtttaaggct gtagataagg
      961 agatggcaag ggggagtaaa cctaattcat tagagttttt acgtgatttt tatataaaag
     1021 catataaggt aatgcgtcct tatatgagag aggaaaacgt tatagtattt catgatgcat
     1081 ttgaattaaa agcatggaaa gactttatga gagaggaaga atttaaaaat gttgttctag
     1141 atacacacca atatttgatg ttagctgaag cagatggatg tgaacaaagt attgatagct
     1201 atctcaaata tattaaagaa aattatgcta aagatatttt acaaatgcaa aaatattttc
     1261 ctgttatatg tggggagtgg agcttattta attcatatgc ttgtggtata gatacaaatg
     1321 gaggtcaatc acctctaaat gggattgaat caaatataga taaattatca aaagacgata
     1381 aaagagaatt atataggaag atagcaaaag cacagcttga tgcatggaga aatggaagtg
     1441 gtcactacta ttggaattat aagttattac tagatacagt taatgaagaa ggttggatag
     1501 gatgggatag ttgggaccta ggcaaatgtg tagctcaaga atggtatcct atagaatatt
     1561 aaactaaaat tggaggaaaa gatatgatta aaaaccccat acttccaggt tttaatccag
     1621 atccttgtat ttgtagaaag ggagatgatt attatcttgt agtttcatct tttgaatggt
     1681 ttccaggtat acctgtttat cactctaaag acttaaaaaa ttgggagtta tatactcata
     1741 ttttgacaga tgaaacaaaa atagatttga agaagcttcc ttcatctaaa ggaatttggg
     1801 caccttgttt gacttattgt gaagaagaag atttatttta catagtttat ggaattatga
     1861 actctatgaa tgcaagatat tttgatgttg ataattattt aattacatca aaagatataa
     1921 agggagagtg gagtgaacca gtatatctac actcatcagg ttttgatgcg tctatttttc
     1981 atgatgatga tggtaaaaag tggattgcat cattagactg ggagactaga gaaggatatg
     2041 aaaaacctgg tgttatatgt ttagtggaat actgtacaaa aaagaaagaa atagtaggat
     2101 atccaaagag aatatggtca gggggaactg atagaggttg tattgaagca ccacatataa
     2161 caaagcgtgg agattactat tatataatgt gtgcagaagg tggaactgga tatggtcata
     2221 gtgtaactat gggaagggct aaaaatatat ggggacctta tgaaaaagat tcaatgaatc
     2281 caattgtaac ttcaattcca ggcgattttt atgaaagaca tgaccctgac catctaaagc
     2341 ctaaatacta taatccagaa tcaaaacttc aaaaatctgg tcatggaagc tatatagaaa
     2401 caacatcagg tgaagtatat ttagttcatc taacatctag accatttgta ccagagttaa
     2461 ggtgtacttt aggtagagaa acagctattc aaaaaatgaa atggacaaag gataattggc
     2521 ttcgaatgga agatgaaagt aatttggcaa aagaatacgt atcagaaagt aaactagaag
     2581 aacatttagt ttcatctata ccaagttttg atgactttga ttcaaatgaa ttaggtttac
     2641 agtactatgc tcctaggatt tcaccattat catttgcaga tgttaagtct agaccaggat
     2701 atgtaaggat tagaggacaa gaatcaagaa catcattaaa taaagtaagc atattggcaa
     2761 gaaaattaac ttcagtatat gcaagaataa ccactaagat ggagttttac ccagaggtac
     2821 atcagcatag tgcgggtttg ataatgtatt atgataatat gaattatata aatttaagaa
     2881 agtattatag tgaaacattg ggtcaaagtg cactatcaat aatacatctt gaaaatggag
     2941 agaagacgga atttttaaat actaggatac caataaaaga tataccaata tatcttcgtc
     3001 tgtatataca aggtcgtaag tcatattttg aatggagtta tgatgaaaaa aactaccaaa
     3061 gaataggaaa ggtatttgat acaactaagt tttcagatga gtactgtaaa tatggagaat
     3121 ttacaggtac gtttatagga ttaacttgtg cagatcgtgt aaagcataaa cactatgctg
     3181 attttgattt ttttgaatat atagtagatg aatcaaaaga tgtcgattaa taaaatcatt
     3241 actaaaattt tcagagcaaa ggtaaatctt atgctctgaa ataagaaaca ttagaaggca
     3301 aatcttacaa aaaggagtat ggtattatga atacagaagt taatgttaaa gcaactacga
     3361 ttagatatga aaagatttca ttttttgaaa gaatttctta tggttgtggg gatttggggt
     3421 gcaatatcat atactcagct atgtcagcat ttttgctgtt ttattatact aattatgcag
     3481 atgttagtgc agcagctgta ggtagtatta tgcttgtatc taggatactg gatgggttta
     3541 gtgacttaac aatgggaatt atagtggata gaactaaatc caaatacggt aaggctaggc
     3601 cttggatttt gagaatggcg attccttttg ctatagcagc agtattatta ttttctgttc
     3661 catcaaatct tggtatcact tctaaattaa tttatatttt tattacatat aacttagttt
     3721 ctactgttat ttatacagca atcaatgtac cctatgcaac tctaaattca cttataactc
     3781 aaaaccagta tgaaagagga gtattaagta tatttagaat gatattagcc acttgtggta
     3841 ccttactaat aaatggactt acactacctc tagttgaata ctttggaaat aatttatcag
     3901 catggactaa aacattcttt gtatttggaa tagcatctat aatggtattt tttataacat
     3961 ttactggaac caaggaaaga gtaaaagcag ttaagcaaga taggaatgaa gtaataccat
     4021 ttaaaatagg tattaaatct ttatttagaa ataagtactg gatacaaata acattatgtt
     4081 tagtttgcat atttattgta tttgcgataa atggtggttc atccgtatat tatgcaaagt
     4141 ttattttggg agatgaaaaa ttatttggtc ctataaatat ggtttcaaat atatcccaaa
     4201 ttatagctat gtttatggtt gctcctttta taaaaaaatt tggaaaaaga aatgtattaa
     4261 tagtaggttc aataatatta atatcgtcaa atgttatgtt tataatagtt ggacaaaacc
     4321 atataggagt aatatttgca agtgctataa agggaatagg aagtgctgga atagcatcaa
     4381 caatgtttgc tattgtttca gatactattg aatatggaga atggaagact ggatatagaa
     4441 ctgaggggct tataaatagt gcatctagtt ttgggtttaa agtgggaaat ggattaggtt
     4501 cagcaatatt aggtgcagta ttatcaattg gtggttatgt aggaacatca gctactcaaa
     4561 gtgatttagc tatagtatca ataaaagtat gttttatata cctacctata tttataacaa
     4621 tattacaaat tatcataatg tctttttata agcttgataa agaatatagt actatactta
     4681 atgaattaaa tactaaataa tgaaaaagtt tcttataaag tcttaag
//
LOCUS       DAEMHW010000247         4712 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEMHW010000247 DAEMHW010000000
VERSION     DAEMHW010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.248"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            770..1522
                     /locus_tag="KPK67_003469"
     CDS             770..1522
                     /locus_tag="KPK67_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460387.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCKLFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            1512..3623
                     /locus_tag="KPK67_003470"
     CDS             1512..3623
                     /locus_tag="KPK67_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460388.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SIFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLISGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEWVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNEEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
     gene            3831..4541
                     /locus_tag="KPK67_003471"
     CDS             3831..4541
                     /locus_tag="KPK67_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2460389.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT         1794 a          643 c          618 g         1657 t
ORIGIN      
        1 atattacaat ctcttattta ttacagttct ttattatact acagactttt attattacag
       61 catttctgtt acattgcaaa gtcttattta ttacagttct ttattatact acaaaccttt
      121 attattacaa cactttatta ttttaacttc tttactataa tatagtaata ttttaatatc
      181 tcaaaatcat atcaatcact tttcagcata gtctaaaata cttccacttt ctaaaattat
      241 ctatcatacc tatactaata atgcttttta acctttttat tatacatttc aattgtttct
      301 aacaacctat ttatttccaa aactccaata tattttctca attacattgt gttaataacc
      361 cctaaatcct ttcaaattat ttacaataca ttccattaaa taatcttact gctctataat
      421 tctttttttc tttttaaccc tactatacat tatttacatc atttttttca atacaacatt
      481 cgttattcta taaccttttt acttttacta tatacttctt ttctaactac accttcaatg
      541 ttccacataa tattacttta gcacaacaat taatacctaa aaataaccct ctaaatcctc
      601 caataatata atcaatttaa ctataataat aacctctcac actatccaaa caatatattt
      661 tcaaacatta caaaaaaaca tccaactatt acaatatatc tcatatccct cctccccttt
      721 caaatgctat cattgactta caagataaat gaaagtgagg atgatatata tgcttaaagt
      781 aaaccaatta tgtaaatcat ataaaacagg tagtaaaaca tatcctgtat taaaaaatat
      841 ttcttttgaa atcaacaaag gagaatttgt cgcaatcatg ggttcatcag gaagtggaaa
      901 aactactctt ttaaattgta tttcatgttt tatcccaatt gataatgggg aaataattct
      961 tggaaataat aatataagaa atttaaaaga aaaggaactt tcagaagtta gaaatcaaca
     1021 actaggattt gtattccaag atttcatgct atttgatggt cttagtgtct ttgaaaatat
     1081 atgtgttcca caaattatca aaggtagtca agtcttacaa atggaaaacc gtgcaattaa
     1141 tctatgtaag ctatttggta ttgaacatat cagaaaaaaa taccctgccg aaatatctgg
     1201 tggagaaaaa cagcgtacag cagtagcaag agcactcatg aacaacccag atttaatatt
     1261 agcagatgaa ccaactggta accttgatag taagtcttgt cgtacagtca ttgaatcatt
     1321 tttaaaagca aagcaagaat tagatgctac tatactcatg gttacacacg atagctttgc
     1381 agcaagcttc tgtgaccgag ttattgtgct taaagatgga ataatttata aagaagttgt
     1441 aaaatcaaat tcaagacata aattcctaga tgaattacta gaagttttaa aagaaatggg
     1501 aggcgctcaa gatgacaatt aataaattat acagtaaact tcgagcctat aattggaaga
     1561 actatacaac tttattcttt tgtataatac tttctgtggt attagtaact tcatatgctc
     1621 taatttattt tagcccaaca atacaaaaca tacttccaac tggtggagat agcagaaaac
     1681 aggctacaat gatattctca gttgctatat taggatgcgc tgtctttact acatacgctt
     1741 cttccctctt tttaaaattt aaaagcaagg aaattggtat tttcatggct ctaggaacag
     1801 ataagaagaa attaaaaaaa cttctatttt cagagatatt gttaatttca atagtatgct
     1861 gttttttagg tcttatatta agtattcctg tatcttttgg tatatggaaa atatttcaat
     1921 tagtgattat tgataccaga gaaatgacat atcattttgg aactcaagga ttcatatatg
     1981 gatttatatt tagtatattt gtgacattat gcatatttat actttctgta aaatttatta
     2041 atcgtacaaa tataattgat atattaaatg accagagaat atctgaacca atacaggatg
     2101 taaaggactg gtatggtata ttaggtataa tttttattat actcggtatt tttctaggat
     2161 atctcgtacc ttcactaact ataaacaaat taaattatag actaccatct gtatggagct
     2221 ttacttatct actctctctt attggaatat atatgattat gacatacata gttgtacatg
     2281 ctaaaagagg aaaacatcca gagaaatact ataaaaacat catatcaact agcatgatga
     2341 gatttatggg aagacaaaca gtaaaaaaca tgtgtgttat ttcatttttg atttcaggag
     2401 cattatttgc tgtattctat gttcctaata tggttacagg tatctatgat actcttaaaa
     2461 ataaccctat agattatact tttacctaca atcaaagaga taaacaggta tcaaaaaaag
     2521 atattgaaca attagcagat aaacacaata taaaaattca aaactatatg gaaattcctt
     2581 ctatagaatt aattgttgat ggtgtagaat caatatatca taaagatgga actatcgaat
     2641 gggtatatat gaaaaaaagc tcctatgata tgttctttaa ttctaaagat ttttccaagc
     2701 taattggcaa accaataact ataaaaaatg gtgaatatct tgctgttgta aataaaggaa
     2761 atgaaaatca agattataaa tataccaaat ttatatcaag cccagtcaca gaaaaagaaa
     2821 tcccctttag atatgctgga aaaataatat ttaatggttc tttttttcaa aatagaagta
     2881 tgaacgccta tgtccttaat gaggaggatt ataaatcatt tactaaagat ttatccataa
     2941 aaaattacaa taattctata ctatttaatg tagataaaga tacctatgct tttgccaatg
     3001 acttaaagaa agaaattata aaaagaacat ctaaggaaat gggcatgttc agtggttatg
     3061 atgaatatgt aaaaaaacgt tatgaatcaa ctggaagaaa atattttatg gatgaagaat
     3121 atccaagtgg tgaaggtcat ttaaaactag acccaaacaa caatcagttg tatcttaatt
     3181 ggaaatacta tcctaacttt aaaattttac aaagtcaaga tatgataaaa aacatggcag
     3241 tcttcttaat gttattcata tatgtggcaa tcatatgttt tacagcagta ggaatcattg
     3301 catatacaag gggtgttact atagctataa atcacaagca ggtctttatg gatttaaatc
     3361 gattaggagc aaacaatgat tatataaagt tctgtattag aaatcaatta aagaaagtat
     3421 tttcataccc tcttattgca ggaagttttt taatttatct gtttaatttt atgattatgt
     3481 taaataatga tggtagaatc gtcaatagtg aggtcaatgc tcttttgata aacttaggtt
     3541 ttatagggat ttgtggggtt tatatgtacc ttatatatct attaacttat aagaaattca
     3601 aaaaaattat tggtatttta taataagaaa ggttattaga tttttaaagt aataaaatca
     3661 ttagcacttc agaataagtt taatagaata gttttaagtg taagtatact aatataaaaa
     3721 attttatttt gctatctgga tataattgtt atttcatttt atatatctag gtagcaaaat
     3781 atattatata cttaactata taaatacaaa acttgaaagg agataaaaaa ttgacttcga
     3841 tttgtatttg tgatgatgaa aaacaacaaa gaaacatgct gaataaaatc attacaagag
     3901 aaatggattt gtggggctac ccctaccaaa taaaagaata ttcaaatgga gaaagtctag
     3961 tatacaatct agaacacgaa aattattaca tagatattat ttttttagat attgaacttg
     4021 gaaaaagtaa tggtgttgac atagcaaaaa ctatacgaaa acattcaaag gatactataa
     4081 tcatctttgt cacaggtttt tctgactatg tatttcatgg gtatgatgta ggagctttga
     4141 actacatctt aaaaccttat aaaactgaaa aaatatgtaa agtattaaaa gaagcactaa
     4201 aaaaattaga gcaatggaaa gaaaacttta ttacagttca gattggaact aatctttgta
     4261 aaattaatac aaaagatatc ctctacttta aaagtgagct aagaaaatta actgtagtta
     4321 cctttgataa aacattagaa tactacggaa aattaaatga tatggaaact gttcttccct
     4381 ctaattttat acgtacgcat caaaggtata ttgtcaatct aaaccatgta gatagtataa
     4441 ctcaaactta tgccaaagtt cagaatgaaa aaattcctat aagccgtaaa aaatatcaag
     4501 aggttgtagg aaaatttaca aggtatatgt tagaatctta atttttgaag ttcaatctct
     4561 agaattttat ttttgaaact taatttttta aagcttagtt tttagagttt tgttttttca
     4621 aatttaattt tttgaagctt aatttctaga atttcaatct ttcaaaattt aattttttga
     4681 agcttagttt ttagagtttt tttcaaactt aa
//
LOCUS       DAEMHW010000248         4671 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEMHW010000248 DAEMHW010000000
VERSION     DAEMHW010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.249"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            166..705
                     /locus_tag="KPK67_003472"
     CDS             166..705
                     /locus_tag="KPK67_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2460390.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            724..1767
                     /locus_tag="KPK67_003473"
     CDS             724..1767
                     /locus_tag="KPK67_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460391.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            1769..2605
                     /locus_tag="KPK67_003474"
     CDS             1769..2605
                     /locus_tag="KPK67_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460392.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            2710..3384
                     /locus_tag="KPK67_003475"
     CDS             2710..3384
                     /locus_tag="KPK67_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460393.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            3384..4436
                     /locus_tag="KPK67_003476"
     CDS             3384..4436
                     /locus_tag="KPK67_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG2460394.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
BASE COUNT         1822 a          500 c          851 g         1498 t
ORIGIN      
        1 aaatgcttgt atttattcta aataaaaaaa taaaaaaatg tcaaaaaaac tcttgtatta
       61 tatccaaatt ttatatataa ttacctatgt tgtttagaaa ttttaaactc aatgtttagt
      121 aaacaaaatg tttagtaaaa taaaacagaa actaggaggt gcaacatgga tataggtgaa
      181 aagataaaaa gattaagaac agaaaaacaa ctgacccagg aagagttagc taatagatgt
      241 gaattgtcaa agggatttat ttctcaatta gaaaataact taacatcacc atccatagct
      301 acccttatag atatacttga aatactaggt acaaatttaa gggaattttt taatgaaata
      361 gatgatgaaa gaatcagttt tacaaaagaa gatatgtttg aaactgaaga tgaagattta
      421 aaatataaat taaagtggtt aattccaaat tcacaaaaaa atgaaatgga gccaataata
      481 ataacattgt atccaggagg acagtataaa gaagaaaagc cacatgaagg tgaagaattt
      541 ggatatgtat tagcaggttc aatatatgtc catataggcg aaaagaaaaa taaagtaaaa
      601 aaaggagaga gtttttactt tagaccgaaa gcaaatcact atatatcaaa cgagggcaaa
      661 actacagcaa aagtgatttg ggtaagtacg ccaccatcat tttagaatag aggtgtttaa
      721 taattggtag aaaatataat agaattaagg gatatatcta aacattatga agaacttact
      781 gttttagata atttcaattt agatataaag aaaaatgagt ttctaacatt acttggacca
      841 agtggatgtg gaaaaactac aacattaaaa ataatagcag gatttgagta tgctgatgat
      901 ggtaaagttt tatttgaagg taaggagata aataatttgc caccatatga aagacaagta
      961 aacacagtat ttcaaaaata tgctttattt ccacacatgg atgtttatga aaatgtagct
     1021 tttggattaa aaattaaaaa gactccaaaa gatgtaatag atgctaaagt aaaagaggtt
     1081 ttgaaacttg ttgcactaga aggttttgaa agacgacaag ttgaatcatt gagtggagga
     1141 caacaacaaa gaattgcaat agcaagagct ctagtcaatg aaccaaaggt attattacta
     1201 gatgaaccac ttggagcact tgatttaaag ttaagacaag agatgcaaat agaattaaag
     1261 agaatccaaa aaaaattagg aataacattt gtttttgtaa ctcatgacca agaagaggct
     1321 cttactatgt cagatactat tgttgtaatg tataagggca aaattcaaca aatgggaaca
     1381 ccacaagata tatacaatga acctaaaaat tcatttgtgg ctaaatttat aggtgaaagt
     1441 aacatatttg atggaattat gatagaagat tataaagtta atttctgtaa tagagacttt
     1501 gagtgtgttg ataaaggatt cgaaaaaaac gaaaatatag aagtagtaat aagaccagaa
     1561 gatataaaaa tggtaaagcc agaagaagga atgttaaagg ggaaagttac atctacagtg
     1621 tttaaaggag tgcattacga gatagaattg aatgaaaatg gaagattgtg gatacttcat
     1681 aatactaaaa atgctgaggt tgggactgaa ctaggcatgg atatatatcc agaagatatc
     1741 catataatga gaaaagagaa taactaagat gaaaagaaaa tcttttttag catatccata
     1801 tgtagtttgg agtgcgatat ttgtaataat tcctttaata ttagtagtat tttttagttt
     1861 tacaaaagaa tcaggtggag gttatgcctt tacacttgag aattataaag aagtaattga
     1921 ccctatatat atgaaagttt ttgggcgttc tattctttta gcaggaggag ctacattaat
     1981 atgccttata gttggttatc cagtggcata tataatttca aaggctcgtg taagtagaag
     2041 aggttcatta atattattgt ttattcttcc tatgtggatg aactttcttt taagaacata
     2101 tgcatgggtt gctatattag gaaaaaatgg acttttaaat acatttttag gttggtttgg
     2161 aatacaacca ttagcaattt tgtatactaa ttttgctata cttttaggta tggtgtataa
     2221 ctttttacca tttatggttc ttccaatata tacagcacta tcaaagatgg ataatgattt
     2281 gataaacgct gctcatgact tgggtgcaaa taatatgaca gtttttagaa aaattatatt
     2341 tcctttgagt ttgccaggtg taatgtctgg aataactatg gtatttatgc cagcagtcac
     2401 tacttttgca atatcaagac tgttaggtgg aggtaaaata atgctggttg gagatttgat
     2461 tgaacaacag tttacagttg ttggagattg gaactttggt tcagctatat cgatatttat
     2521 gatgatagta atattaatat ctatgtctat aatgtcaaaa tttggagatg aatctgacaa
     2581 agaaggcggt gggttactat tttaaaatta aagaaaataa cttctaatat atatttagca
     2641 ttagtattct tatttttgta tgcacccata tttgctttgg tactattttc tttcaatgat
     2701 tcaaaatcaa tggcaagatg gaatggattt acatggaagt ggtatggtca attacttcaa
     2761 aatgagagta taatgagtgc tttatattat actattgtaa tagctatatt agcgtctgta
     2821 atatctacaa tagtaggaac tataagtgct ataggaatac ataagatgag aggtaagagt
     2881 aagaaattaa tattaaatgt aaactatctt cctattttaa atacagagat agttacagca
     2941 gtagctctta tgagtctgtt tgtatttgta aaaatggaat ttggatttac aacaatgcta
     3001 ttggcacata taatgttttg tttgccatac gttatacttt cagttttgcc aaaaatgaag
     3061 caattacctg ataatataga agatgcagct atggacttgg gagcaacacc tatatacgct
     3121 cttagaaaag taattttacc acaaataaaa ccaggtatag tttctggatt tttaattgca
     3181 tttactatgt caatagatga ctttataata agtttcttta atgctggaaa tggtgtaagc
     3241 aatctttcta tagaaatcta tggaatggct agaagaggta taaaacctga aataaatgca
     3301 ttatcaacaa taatgtttgc agtggttcta ggtttacttt tactagctaa taaaaaagaa
     3361 tctatagtaa gggggattaa ataatgatta agtttaaaaa agcaatatcc cttatagctg
     3421 taggtatgat gacagttacc ttatttacag gatgtaataa agcaactgat tctacaaaag
     3481 ttttaaatgt atataatgtt ggagattata tagatgagtc tcttatagat aagtttgaaa
     3541 aagaaacagg aatagatgtt caatattcaa cttatgatac taatgaaatg atgtatcaaa
     3601 aagtaaagag tggtagtaca cactatgatt tagtatttcc ttcagactat atggtagaga
     3661 aaatgaaaaa tgaaggtctt ttggaaaaat tagattttaa gaatatacct aatatgaaat
     3721 atttagataa gagtttttta aatcctgtat acgacaaaac taatgaatat agtgttgcat
     3781 atatgtgggg aactcttgga atattgtaca ataagaaaga agttaaagac cctatggata
     3841 gctggaatat actttggaat ccaaagtata agggaaatat aatgatgttt gactctgtta
     3901 gagatactat agggataact cttaaaaaat taggatatag catgaatagt gtaaatccaa
     3961 aagaaataaa tgaagctaaa aatcttttaa tgaaacaaaa ggatttagta ttggcttatg
     4021 taaatgatga aggtaaggat agattacttg gtggagaagt ggcaatgggt atgctgtatt
     4081 ctggagatgc agttacacta atggagcaaa atcctgacct tgattatgct atacctaaag
     4141 agggaactaa taaatgggta gatgctatgt gtgtacctac aacagcacaa aataagaaag
     4201 aagctgagct gtttataaac tttttattag acccagagaa tgcaaaagta aatgctgagt
     4261 acattgggta ttcaacacct aatacaggag cactaaaatt attagaccca gaaataactc
     4321 agaatcctgt tgcatatcca tctaaagaag tacttgataa atgtgaaact tttgaagata
     4381 ttggtgaaaa tataaaatta tatgacaagg cttggataga gttaaaatca aaatagggtc
     4441 ttgttgtttt aagtaaattt acttaataaa tacttaataa aactgttctt ttttagaaaa
     4501 tttattttct aattattgaa cagttttatt ttttgtttaa gttatattca tatgcaattt
     4561 aaatagttta ttaataaatg aaaacttatt tgtataatga ctttaataat aattagaaaa
     4621 aatgggcaac ttaatagaat aattggaggt gtaaatttat aaaaaatgaa t
//
LOCUS       DAEMHW010000249         4611 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEMHW010000249 DAEMHW010000000
VERSION     DAEMHW010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.250"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            98..1072
                     /locus_tag="KPK67_003477"
     CDS             98..1072
                     /locus_tag="KPK67_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2460395.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKELSLKAKGLLSQMLSLPEDWD
                     YTLAGLSLINRESIDAIRTAVWELEKAGYITRRQGRDEKGKMTAIEYTIYEQPQPPTL
                     DCPVLENPTADKPILENPTPDNPTSENPTQLNKELLKTNLPKKEKLNTDISNTHSIPF
                     HSLNPSPLEDAAQPPERKRKETTDAYRVYEEIIKDNIEYEHFIKHTDFEKERLDEIVS
                     LILETVCTKRKTIRIAGDDYPAELVKAKFMKLNSSHIEFVFACMSENTTKIRNIKQYL
                     KAVLFNAPNTIDSYYTALVNHDMYGGRTIQSSRSKGIPDYTYNEGESL"
     gene            1092..1310
                     /locus_tag="KPK67_003478"
     CDS             1092..1310
                     /locus_tag="KPK67_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBG2460396.1"
                     /translation="MTQKTGALIFDETADRYDIRFDVNDYYGGLHCGDCMEVFVRGKW
                     KPTRMEYGDNWYLVGVRASDLNGLRVRI"
     gene            1404..1886
                     /locus_tag="KPK67_003479"
     CDS             1404..1886
                     /locus_tag="KPK67_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBG2460397.1"
                     /translation="MQEETNEKTIALYIKTGKLTAQQLQKAMKALLAQMKKQHDKQKI
                     PHGKQTIKQLMKQNAGVSNIEITKDNIKAFESTAKKYGIDFALKKDSTETPPRYLVFF
                     KGRDADTLTAAFKEFSAKKLTQEQKPSIRKLIVSLKEKAAALNAQRDKVKNKDRGIAR
                     "
     gene            1883..3673
                     /locus_tag="KPK67_003480"
     CDS             1883..3673
                     /locus_tag="KPK67_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBG2460398.1"
                     /translation="MNAINWKKLIIPNIPYLLFVYLFDKVGQAVRLSPGADLSGKVLS
                     LADGFSAAFANPLPSLAPMDLLIGIAGAVLIRLMVYFKGKNAKKYRKGIEYGSARWGN
                     AEDIKPYTDPVFQNNVLLTQTERLTMNSRPKQPKYARNKNILVIGGSGSGKTRFFVKP
                     NLMQMHSSYVVTDPKGTVLVECGKLLQRGGYRIKVLNTINFKKSMRYNPFAYLRSEKD
                     ILKLVNTLIANTKGDGEKAGEDFWVKSERLFYCALIGYIWYEAPEEEKNFTTLLEMIN
                     ASEAREDDPEFQSPVDLMFERLEEKDPEHFAVRQYKKFLLSAGKTRSSILISCGARLA
                     PFDIKELRDLMETDEMELDTIGDRKTALFVIISDTDDTFNFVVSILYTQLFNLLCDKA
                     DDEYGGRLPVHVRCLLDEFANIGQIPKFEKLIATIRSREISASIILQSQSQLKAIYKD
                     NADTIVGNCDTTLFLGGKEKTTLKEMSEILGKETIDSFNTSENRGREVSHGLNYQKLG
                     KQLMTEDEIAVMDGGKCILQLRGVRPFFSDKYDITKHPNYKYLSDYDKKNTFDMEKHL
                     RRRPALVKPDEVFDYYEISESDLQEDTDHE"
     gene            3666..3833
                     /locus_tag="KPK67_003481"
     CDS             3666..3833
                     /locus_tag="KPK67_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460399.1"
                     /translation="MNRRIRKKKDKKITGAINGLVSIAERREQQRQAAIRQFVKRCEA
                     LYEQQRKERRN"
     gene            4033..4248
                     /locus_tag="KPK67_003482"
     CDS             4033..4248
                     /locus_tag="KPK67_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBG2460400.1"
                     /translation="MEFFNSAIGVLQTLVIALGAGLGIWGVINLLEGYGNDNPGAKSQ
                     GIKQLMAGGGVALIGTILVPLLSGLFG"
     gene            4265..>4611
                     /locus_tag="KPK67_003483"
     CDS             4265..>4611
                     /locus_tag="KPK67_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460401.1"
                     /translation="MQSILDAINEWIKEILIGAINGNLSTMFGDVNEKVGTIANEVGQ
                     TPQGWNANIFSMIQTLSENVIVPIAGLVITYVLCYELISMVTEKNNMHDIETFMFFKW
                     IFKAFVAVFIVTST"
BASE COUNT         1384 a         1081 c         1128 g         1018 t
ORIGIN      
        1 ttagggcgtg aaaatgagaa aggcaagggt tgataccttt tccctcaaaa ccgcgtttct
       61 actgcgtaac aaatccaacc aaaggagtga tgaagctatg gcagttttcc gcgtggaaaa
      121 gaataaaggt tatacggtta tgagcaacca ccatttacga aacaaggagc tttccttaaa
      181 agcaaagggc ttgttgtcgc aaatgctttc acttcccgaa gattgggact atacccttgc
      241 agggctgtcc cttatcaacc gggaaagtat cgacgctatc cgcaccgccg tatgggagct
      301 tgaaaaagcc ggatatatca caaggcggca gggacgcgac gagaaaggca aaatgaccgc
      361 tatcgagtac accatttacg agcagccaca gcccccgaca ttggattgtc cggtattgga
      421 aaatccaaca gcggataagc cgatattgga aaatccgaca ccggataacc cgacatcgga
      481 aaatccaacg caattaaata aagaactatt aaagactaac ttaccaaaaa aagaaaaatt
      541 aaatacagat atatcaaata ctcattccat tcctttccat tccctaaatc cctctccttt
      601 agaggacgcg gcacagccgc cggaacggaa gcgaaaggaa acgacagacg cataccgagt
      661 gtatgaggaa attatcaagg acaatatcga atatgagcat ttcatcaagc acactgattt
      721 tgagaaagaa cgcttagacg agattgtgtc ccttattctt gaaacggtct gcaccaaacg
      781 aaagactatc cgtatcgccg gggacgatta tccggcagag cttgtgaaag caaagtttat
      841 gaagctgaac agcagtcata ttgagtttgt ttttgcctgt atgagtgaga acaccaccaa
      901 aatccgcaac atcaagcaat atctgaaagc ggtgctgttc aatgccccaa acaccattga
      961 cagctattac accgcccttg tcaatcacga tatgtacggc gggcgtacta tccagtcgtc
     1021 aagaagcaag ggcatacccg attacaccta caatgagggc gaaagcctgt aaaccaaagg
     1081 aggattttat tatgacacaa aaaacaggag ctttgatttt tgatgaaacc gctgaccgtt
     1141 acgacattcg ctttgacgta aacgactact acgggggctt acattgtggc gactgcatgg
     1201 aggtctttgt gcggggtaaa tggaagccga cccgcatgga gtacggggac aactggtatc
     1261 ttgttggtgt gagggcttcg gatttgaacg gactgcgggt tcggatttaa cagggcggcg
     1321 tgaaaacgga cgctcttttt tcatgcccgc cccactttcc ggcggcgggc ataccaccat
     1381 agaacacgaa aggaggaacc ccattgcagg aggaaaccaa cgaaaagacc atagccctct
     1441 acattaagac cgggaagctg accgcgcagc agcttcaaaa ggctatgaaa gccctgcttg
     1501 cacagatgaa aaagcagcat gacaaacaga aaatcccaca tggaaagcag accataaagc
     1561 agcttatgaa gcagaacgcg ggcgtttcca acattgaaat cacaaaagac aatatcaaag
     1621 cctttgagag tacggcgaaa aaatacggga ttgactttgc cctcaagaaa gacagcaccg
     1681 aaaccccgcc ccgttatctt gtattcttca aaggacggga cgcggacaca ctaaccgcag
     1741 cctttaagga gttttccgca aagaagctga cacaggaaca aaagccctct atccgcaagc
     1801 tgattgtttc cctcaaagaa aaggcggcag ctctgaacgc acaacgggac aaagtaaaaa
     1861 acaaagacag ggggattgca agatgaacgc tatcaactgg aaaaagctga taattcccaa
     1921 cattccctat ctgctctttg tctatctctt tgataaagtc gggcaggcgg tacgcctttc
     1981 tccgggagct gacctttccg gcaaggtgct ttcccttgca gacggctttt ccgctgcctt
     2041 tgcaaacccg cttccgagcc ttgcccctat ggatttgctt atcggtattg cgggggctgt
     2101 ccttatccgg cttatggtgt atttcaaagg caagaacgcg aagaaatacc gaaaaggtat
     2161 tgaatacggt tctgcccgtt ggggcaacgc cgaagatata aagccctata ccgacccggt
     2221 atttcaaaat aacgtgctgc tgacacagac ggaacggctt accatgaaca gccgcccgaa
     2281 gcagccgaag tatgcaagga ataaaaatat tcttgttatc gggggaagcg gcagcggaaa
     2341 gacgagattt tttgtgaagc ccaacttaat gcaaatgcac agcagctacg ttgtaaccga
     2401 cccgaaagga acggttttag tcgagtgcgg gaagctctta cagcgcggcg ggtatcggat
     2461 aaaggtgctg aatacaatca atttcaagaa atccatgcgc tacaatccct ttgcctatct
     2521 ccgcagcgaa aaagatattt tgaaactggt aaataccttg attgccaaca ccaaagggga
     2581 cggggaaaaa gccggggagg atttttgggt aaaatcggaa cggctctttt actgcgccct
     2641 tatcggctac atttggtacg aagccccgga ggaagaaaag aacttcacga cgctgcttga
     2701 aatgataaat gccagtgaag cccgcgagga cgacccggaa tttcagtccc ccgttgacct
     2761 catgtttgaa cggttggagg aaaaagaccc ggaacacttt gccgtccggc agtataagaa
     2821 attcctgtta tctgcgggaa agacacgaag ctctatcctc atttcctgcg gtgcgcggct
     2881 tgcccctttt gacattaagg agctgcgcga ccttatggaa accgacgaaa tggagcttga
     2941 caccataggc gaccgcaaga ccgccctgtt tgttatcatt tccgacaccg acgatacctt
     3001 taactttgtt gtgagtattc tttacacgca gcttttcaat ctgctttgcg acaaggcaga
     3061 tgatgaatac ggcggcaggc tgcccgtcca tgtgcgctgt ctgttagacg agtttgcaaa
     3121 tatcgggcag ataccgaagt ttgaaaagct catagccacc atacgaagca gggaaatttc
     3181 cgcgtcgatt atcttgcagt cgcaaagcca gttaaaggca atctataagg acaacgccga
     3241 taccatagtc ggcaactgcg acaccaccct tttcttgggc ggcaaggaga aaaccaccct
     3301 caaagaaatg tcggaaatct tggggaaaga aaccattgac agcttcaaca cttccgagaa
     3361 ccgggggcgc gaggtatcac atgggctgaa ctatcagaag ttaggcaagc agcttatgac
     3421 cgaagatgaa atcgcggtta tggacggcgg taaatgtatc ttgcagctta gaggggtgcg
     3481 cccgtttttc tctgataagt acgacattac aaagcacccc aactataaat atctttccga
     3541 ctatgacaag aaaaatactt ttgatatgga aaagcattta aggcgcagac ccgcccttgt
     3601 gaagccggac gaagtatttg actactacga aatcagcgaa agcgatttgc aggaggacac
     3661 cgaccatgaa tagacgtatc agaaagaaaa aggacaagaa aatcacagga gccataaacg
     3721 ggcttgtttc gattgctgaa cgacgggaac agcagcggca ggctgctatc cggcagtttg
     3781 tgaaacggtg tgaagccctg tatgagcagc agcgaaaaga aaggaggaat tgacgcagta
     3841 acaaagaaaa agacaacttg catgatacgc gcccctacaa aaattcctat cgcccacact
     3901 gacaactgaa taccgccgcg cggcagaact ttacgcagcg cggggactta tgaccgcggc
     3961 agtttgaaaa ctgaccgccg ttttttatgc ccttttacgg ggcaagccgc atttgcggca
     4021 gaaaggagtt ttatggaatt tttcaacagc gcaatcggcg tattacagac tttggttatc
     4081 gctcttggag caggacttgg catttggggc gttatcaatc tcttagaggg atatggaaat
     4141 gacaatccgg gtgcaaaatc acagggcatt aaacagctca tggcgggcgg cggcgttgcc
     4201 cttatcggca ctatccttgt acctctgctt tccggcttgt tcggttaagc gcgggtagct
     4261 gcctatgcag agcatacttg acgcgattaa cgaatggata aaggaaatcc ttatcggagc
     4321 gataaacggt aatctgtcaa ctatgttcgg ggacgtaaac gaaaaagtcg ggactatcgc
     4381 aaacgaggta ggacagaccc cgcagggttg gaacgcgaat attttcagca tgatacagac
     4441 cctttctgaa aatgtaatcg tcccgattgc ggggcttgtc attacctatg tcctatgcta
     4501 cgagctaatc agcatggtaa cggaaaagaa caatatgcac gacatagaaa cattcatgtt
     4561 ctttaagtgg atattcaaag cctttgtcgc ggtgtttatc gtaaccagca c
//
LOCUS       DAEMHW010000250         4601 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEMHW010000250 DAEMHW010000000
VERSION     DAEMHW010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.251"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(124..4266)
                     /locus_tag="KPK67_003484"
                     /pseudo
     CDS             complement(124..4266)
                     /locus_tag="KPK67_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897035.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
BASE COUNT         1360 a          797 c          683 g         1761 t
ORIGIN      
        1 tattacgaat gtggtccata tcaaagatag aaaatttatt aatatttata aaatatttta
       61 ctcatatgtg aatttatttt ttatttcagt ttaatttaat gtatacaaaa ttattttata
      121 aaatcaacac caaaagtcat ttttacaaca attacagcaa taatctttac aaccacaatc
      181 attgcaacaa caattactac agttgttaca acagcaatca ttacaacaac attgttttat
      241 ttctacttgc actcttgata tattattgtt aaggtttgaa tctggtgttg ttgaagtaac
      301 ttctgcaata tttgttaatc tatcaagaca tgttgatttt ataattcctc ttaatagaat
      361 aactcttaaa gttccagctg atagtgtacc tatattaaga cttccagtcc atggttggaa
      421 acttacacct ctatcaagtg aaaatactgc tttcttaagc ttatctgaaa cattgtctat
      481 taaagtaaca ttttgagcat tttctggtcc tgcatttgat actactatta caaaatctat
      541 ttgctctcct acacatgctt ctgttttatt tgctaatttt attacagaaa tatctgctga
      601 tgaacttata tctatttctg atattgaagt attattatct ggatttgggt caggtgtagt
      661 tgaacttact gttgctgtat ttataattgt acctattgct gtttgactta ctattcccct
      721 tattataata tttcttatct ctccagcatc taaagtgcct atactaagac ttccattcca
      781 tggttgaaaa gtcactccat tatctattga atattctgga cttaaaatag aagctggtat
      841 attatctgtt aaagttacgt tctgagcagt agacggtcct gcatttctca cctcaattgt
      901 atagtctagt acatctcctg gtacaacaac agtctgattg cttcgtttta ctacagaaat
      961 atctgctgat gaacttacct ctgcttctga tgttgaagta ttgttattta gatttgggtc
     1021 aggtgtagtt gaacttactg ttgctgtatt tataattgtg cctattgctg tttgacttac
     1081 tgttcccctt attatgatat ttcttatctc tccagcatct aaagtaccta tattaaggct
     1141 tccattccat ggttggaaag tcactccatt atctattgag tactctggat ttaaaataga
     1201 agctggtata ttatctgtta aagttacgtt ctctgcaaaa gatggtcctg catttgcaat
     1261 ttcaattcca taaataaatc ttccccctgg tatagcaact ggttcgttgc cttttatcac
     1321 agaaatatct gctaatggac ttatatctgt ttcatctgtt gaagtattat tatctggatt
     1381 tgggtcaggt gtagttgaac ttactgttgc tgtatttata attgtaccta ctgctgtctg
     1441 gctcactgtt cctcttatta taatacttct tatttctcca gcatccaaag tgcctatact
     1501 aagatttcca ttccatggtt ggaaagttac tcctccatcc aatgaatact ctggatttaa
     1561 aataacatct ggtatatcat ctgtcaaaac gatactttgt gcagcagatg gtcctaaatt
     1621 tgtaactgtt atagtataac taaaagtttc tcctggaact gctgtatcca tattgctagt
     1681 ttttattact tctatatctg ctgatggaac tataggagtt gtcactgttg atgaatccgt
     1741 aactggttct ggtacatctg cacttacttc tgctgtatta gtaatatctc ctgtagcagt
     1801 tgaacttact aatcccttta tgataattct tattaattgc cctggtgcaa ctgtacctat
     1861 attaagactt ccagtccatg gttggaaagt tgctcctcca tccaatgaat attctggatt
     1921 taaaataaca tctggtatat catctgttaa aactacattc gtagcattag atggtccttc
     1981 attaaaaatt tcaattgtat atacaaattc ttctcctgga acagctacat cctcgtcagc
     2041 tacttttata atagaaatac ctgcttgtgg gttaactggt gtaataatgg tagatatatt
     2101 gttgcttaaa tctgggtcag gtgtagttga agaaactgtt gctgtattta ctatatattc
     2161 tgaagaattt aaactaactg ttccttgaat taaaactgtt aatgaaattc cagagtttaa
     2221 agttcctata ttaagacttc cattccatgg ttgaaaactt gctcctccat ccaatgaata
     2281 ttctggattt ataatagaag ctggaatatc atcatttaat attacatttt cagcatttgc
     2341 tggtcctaag ttttctactt gaatagtata tctcaataaa gtacctggaa cagctgaatt
     2401 aggttctgct gttttcatta ctaaaatatc tgctaataaa ttcacttctg tctctattgt
     2461 agatgtatta ttattaaggt ttgggtctgc tgtagttgaa gatactacag ctgtatttgt
     2521 tataattcct actgttgatg gatttacaat acccctaatt ataataactc ttgtctctcc
     2581 tacttctaat gtccctatat ttaaacttcc attccatggt tgaaaagtta ctcctccatc
     2641 taatgaatac tctggattta taatagaagc tggtacatca tctgttaaaa ccacattctc
     2701 agatgggaat ggtccattat ttactacttt tatagtgtat attaattcct ctcctggatt
     2761 cactatagta ggttcagata attttgtaac ttcaatatct gctgatatta cttcaacagg
     2821 aactggatta gaatctactg aaaaatcatt tggtataccg ccttctacag gtgtatagga
     2881 gtaatctata ttagctatat tattagtagg atttgggtta ggaatacttt caaccacaac
     2941 atcaaaagtt acttcaaccc ctgtccctcc tccaatatct ggaagtggaa aacctgtatt
     3001 agggtctact cctggtacag atactccatc aaccgttaca gttccaggta taaactctaa
     3061 accatttgga attaaatccc taaaaaatac attagttaaa gggctatcac aagtattatc
     3121 taatgtaact gtatatgtga ttatatcacc tacattagca gtttctgtgt tacttgtttt
     3181 tattggagta aaagttggaa cagctatttc tcttaaagaa acatcatcta tagcatagtc
     3241 atttccaatg gcgctttctc cttcactaaa aaattctact gtaagttgat tattattttg
     3301 agaattaatc acagtaccta cttatttcca ttctggtgcg tttgtattaa ctggaatttg
     3361 agctcctaat gttgcttgat atagtatatt attattttgg tcaaatattc ttacacctag
     3421 ctgtggtggt ggatatcctg ttaccttaaa aaggtttaaa atccaagtgc taaataaata
     3481 attagtgtta ggtgtaactt gtactgttga tttaaaaaat acagctccag gattaaaacc
     3541 atttaccacc atcattcttc ctgtttcatt tcctgtagta tggtcagcaa tacgccacca
     3601 agctcctata acattactca ttgcatttgt cataatattt tgtactgtat attctccatc
     3661 taaaggtgta aacttagttg ggtctggtat aacatacgta aaatctggcg tcacatttgg
     3721 gtaaggttct atttcaggtc ctgtattact tggtgtacct ggtggaaaaa aaccgaatgt
     3781 tccgttatct gcatctgtaa taaggtttat attagataca gatacgcaag aatccaatat
     3841 attctctata ggtgtataaa tcacaggccc atttaatata ttttgatttg ttatatctga
     3901 acctgctact gttattaata gtgtgttagg tgttgtacta tctaagtttg ttgaaccaat
     3961 aggaggattt gtcacagaac taataggtgg catttctgca attggaactg gacctactat
     4021 cgctgttgca aaatcccctg gtgttggtac tcctcctgta gttccaaaag cttctacaat
     4081 tctatagtta ccatttggaa cattagtaaa aacataattt cctgatgcat ctgtgagaac
     4141 cacaagtcta actccagttg ctacatcttg taatacaact ggtatattgg ctattcctga
     4201 gtctccagaa tcaatggtag cacttctatt tctatcaaaa actatttgtc ctgatactgt
     4261 tgccatatta tcactccttt gtgattacaa aatttgtgtt cataatatat tattctttaa
     4321 aactctttta tgtgacattt taagcctata agtgcgcttt ttttctataa catctaatat
     4381 gtattttttt catttgcaat atcaattttt atatatttta ttgtatatgt taaaaaatat
     4441 cttatgtcat aagttaaaaa ctatcaaata atagtaaatg gcataaaatg tattatttta
     4501 tgccattttt caataatcaa gtattttcaa tgtatttata ataataaaat tacaatttgt
     4561 cagtgaaatg tcagtgattt ttttgttatt ttttattctt t
//
LOCUS       DAEMHW010000251         4587 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEMHW010000251 DAEMHW010000000
VERSION     DAEMHW010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.252"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            37..1152
                     /locus_tag="KPK67_003485"
     CDS             37..1152
                     /locus_tag="KPK67_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG2460402.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDAINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIIAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            1338..2234
                     /locus_tag="KPK67_003486"
     CDS             1338..2234
                     /locus_tag="KPK67_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG2460403.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            2304..3101
                     /locus_tag="KPK67_003487"
     CDS             2304..3101
                     /locus_tag="KPK67_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460404.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(3156..4508)
                     /locus_tag="KPK67_003488"
     CDS             complement(3156..4508)
                     /locus_tag="KPK67_003488"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBG2460405.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
BASE COUNT         1726 a          566 c          789 g         1506 t
ORIGIN      
        1 tatcttgaaa tacctattat attatggagg aaaattatga taaacgagca aagaatttta
       61 aatgagtttc ttgaattagt acagatagat agtttatctt taaaagaggg gaatgttgct
      121 aaagtattag ttaaaaaatt agaagaaata ggttgttcag tagtaataga caatgctggt
      181 gaaaaagcaa atggagaaac tggtaatata atagctacat taaaaggaaa taaagaagga
      241 aagaaaatat tatttagttc acatatggat acagtaactc caggtatagg ggtaaaacct
      301 atagtggatg aagcaaatgg aataataaaa agtgatggta ctacaatatt aggttctgat
      361 gataaagcag gtatagcagc tatattagaa ggcttaagat atataaatga aaataacata
      421 gaacatactg atatacaagt tgtattctct atatgtgagg aatgtggatt agttggagct
      481 aaaaatttag actatggaaa aatagattct gaatatgcat ttatattaga tagtggtggt
      541 tctccaggtg aaataatagt aaaagctcct gcacaagatg caataaatgt taagatatta
      601 ggaaagactg ctcatgcagg tcttgaacct gaagctggaa taagtgctat aatggtagct
      661 gccagagcaa tagaaaatat gaatttactt agaatagacg aagaaacaac tgctaacata
      721 ggaattataa acggtggaac agctacaaat atagtaacag gagaagttaa tataattgca
      781 gaagccagaa gtctaaaaga aaacaaatta gatgttcaaa ctaaacacat ggtagagact
      841 tttgaaaaag ctgcaaaaga ttttggagct caaattgaga tagatgtcaa tagagcatat
      901 acaccaatag atgttagtga ggattctgaa ataataaaac ttgcaaagaa ggctttttca
      961 aacttaggta tagaaggtca tactgaatct acaggaggag gtagtgatac aaatatatta
     1021 agtaagaatg gtatcgaagc tataacttta ggaattggta tgaaaaatgc tcatacacta
     1081 agtgaacata tagctataaa agacttatat gattctgcta aaatggtagt ggagataata
     1141 aaggaagcat aaatttaaat aatagtgtat aatatatata tcgcctagct taagctaggt
     1201 gatttttcat tttatagaag attgataatc tctattgaaa gcaaagagta tacatttgca
     1261 tataagagag tgtttaattt atgttcaaat gtaggttctt aataatattt agattgtttc
     1321 ataagggagg aataaatatg gaaacaaggt atgaagaagc aaataaaata acaatacaat
     1381 caatactttg gaatgtagta ttaacaataa ttaaggttat agctggtgtt attggtaatt
     1441 ctagcgctat gattgcggat ggattacatt cagcatcaga tattataagt tctataggag
     1501 tactaatagg caattatgta tcttctagac ctggagatag agagcataat tatggtcatg
     1561 agaaagcaga gacattagta tcatttgtgt tatcaatatt gcttattttc gtttctataa
     1621 caataggtat agaggcaatt aaatctttat ttaacttaga tgcattaagt gttccatcaa
     1681 tattaccatt agtagtatca gttatttcaa tcctgataaa agagtatcaa tatagaataa
     1741 caataaaagt tgctaaaaag ataaattcac cagccctaaa agctgatgct tggcatcata
     1801 ggtcagatgc tttatcttca gtagctgctt ttataggtat aggtggttct attttagggt
     1861 ttaaaccatt agacccaatt gcgtcagttg tagtggctat atttgtagct aaagtaggaa
     1921 ttagtatact tataagttct gttaatgaat tgatggatgt ttcagttgat gaagaagaga
     1981 taaaagaatt aaaatttatt gtagcagata cagaaggtgt taaaaatctt ggtgatataa
     2041 agactagaaa acatggagct atggcatatg tagatttgac tatatgtgtg gatgaaaatt
     2101 tgacagttaa gcaaggtcat gatattgcta ccaagcttga aaaacatata ataaaacata
     2161 tggaatttgt aaaaggcata acagttcacg ttgaaccatg tactaattgc caaggaaata
     2221 agtgtaataa ttaattaaaa cttaaaaaaa agtgtaaaaa gttatataat tgttttatga
     2281 ttataaactc taggggtggt taaatgagca agaaaagaaa actaaagaaa aaagctgcaa
     2341 tattaatagg agtagttgtt ttctttataa tatactttat atcctttaga ataggaataa
     2401 atatggaaaa ggataaaagt gaagtgccaa agaaggtaga aaaagaagaa gaagataata
     2461 ggtctttaag gactcaaata aaagagaaga agaaggttta tatatcagat aaaaatatag
     2521 aaaacataaa aattgaagaa gatatgtggg atgaaataaa gttatttttc tcagatttta
     2581 agaaagtaag aaatactact tcttcttacg aagcagttta tgaaggttat tctgatgatg
     2641 gagttaaatt ttctactgac cttaactttt ttagagtata tactgtaaat aaagaagaat
     2701 attataaagt accagttagt tctaaggatg cttttaagaa acttttagat gagagtatat
     2761 atacttcgtt tgacctttta agtcattata aagattggaa agaggttact ataactcatg
     2821 gaaaagaaat taaaaaagtt catagatgga aatttgatga tttagcacat aagatgattt
     2881 caaagagggt tgtaggaaaa gttcaacctg agaaaagtaa ggaaagaagt gactataatt
     2941 ttactataaa tataaaaggt gataattttg atgtaaaagt tgatacaatg ggaaaagatt
     3001 atgtaaaagt tacttctaag aaaaatgtat cttactatga ggttcataca agtttatttg
     3061 attaccttaa aaatgagata tttgaaattg gaaagaaata agactttaag aaggctatca
     3121 aattagatat ttgatagcct aaattatttt atatattatt tttcaaccaa caagtctgca
     3181 accttataaa ttggaccagc accagcagtt attactaaat cattatcctt aacattttca
     3241 cgtaagtatt ttactatatc ttcaaattct tttatataga tggcatctac attgttttga
     3301 tataatttct caaccaagtc cttagaatgt atatctcctg ggtctttttc cctagcagca
     3361 tatatatctg ttatgataac tttatcagca gcatagaatg cctctgaaaa ttcacctaaa
     3421 agagattttg ttctagtata agtatgtggc tggaagatac accataaagt tgattttttt
     3481 aattttttgg cagcagctaa agttgctttt aactcagttg gatgatgtgc ataatcatct
     3541 actactaaag cattcttata atacccttta gtttcaaatc ttcttccgac acctttataa
     3601 atttttatat tttttcttat agtctctaag tcaataccag aaacaagagc tgccattatg
     3661 gcagaagaag cattgtatat gttatgaaga ccatatacag aaagttcaaa ttctcctaaa
     3721 tctcttccct catattctat tctaaatatt ccatgaccac tattgtcaaa gtgaatatct
     3781 tttattacag catcattgtc agaatctctg ccgtatttta taatagtagc tttaacatca
     3841 tacaatatgt catcaatatt ttcatcatcg ccatttatta tgaaatagcc ttctgggggt
     3901 aataattttc caaatttgtt aaaagaagcc ttgatttcat ctattccaga gaaataatca
     3961 agatggtctt cttcaacatt taaaactata gatatctttg ggttaaaatt aaggaaacta
     4021 tctacatatt cacatgcttc agttataaag tggttggaat tacctatctt aacattacca
     4081 cctatcatac ttaagttacc accaactagt atagttgggt ctaagtctgc atattcaaag
     4141 attgtagata acatagatgt tgtagaagtt tttccatgtg ttccagaaac tgctatagaa
     4201 tttttatatt ccctcatgat ttggcctaaa aaagtcgctc tgttcattac caatttattt
     4261 ttttctttag cagccacaag ttcttcattg tctggatgaa ctgctgctgt ataaacaacc
     4321 atatttacat catctgatat attatctttt ttctgaccaa tataaatagt tgccccttgg
     4381 tccctaagtt tgtctaatag gtatgattcg tttgaatccg aacctgaaac ttgataacct
     4441 ttattgatac atatttcagc taatgcactc atgctgatac cgccaattcc tataaagtgt
     4501 atattcataa taaaaccacc tttcttttta attaagtcct acaatatgaa ctatttctgt
     4561 ggtataataa tataatgttc aatattt
//
LOCUS       DAEMHW010000252         4580 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEMHW010000252 DAEMHW010000000
VERSION     DAEMHW010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.253"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            156..824
                     /locus_tag="KPK67_003489"
     CDS             156..824
                     /locus_tag="KPK67_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460406.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            983..1489
                     /locus_tag="KPK67_003490"
     CDS             983..1489
                     /locus_tag="KPK67_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBG2460407.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1617..2633
                     /locus_tag="KPK67_003491"
     CDS             1617..2633
                     /locus_tag="KPK67_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG2460408.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2987..3691
                     /locus_tag="KPK67_003492"
     CDS             2987..3691
                     /locus_tag="KPK67_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBG2460409.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYVGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            3986..4465
                     /locus_tag="KPK67_003493"
     CDS             3986..4465
                     /locus_tag="KPK67_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG2460410.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT         1924 a          384 c          768 g         1504 t
ORIGIN      
        1 acttaattta tggcatttgt acttgattta ttttaatgta aataatttca aagttaattt
       61 tattcaatat agtactataa aagtataaat aacatttaat aaaaatatat attatattag
      121 gtattttaaa taaatttttt aaagggtgat tttttatgga aaatataact agttatttta
      181 caacaatact atgtgtattt atatgcttat catctgtatt tatatttact caactagcta
      241 gagtatttat taataagaag aagattaatc aaaaaattaa atcaagaaac ggatttaggt
      301 atgatagaga ttttattgaa gccagaagag aagaaataca tataaaggat aacaataata
      361 ataaaaataa gagtaacaat aagaaattaa aggaagaaaa agtatttaaa tacgataatg
      421 gtgatttata taaaggtgag tttgttgatg gaaagaaaaa tggatttgga atatatattt
      481 tttcaagtaa ggaaaagtat gaaggattat ggaaagatga caagatgcat ggaataggaa
      541 aatatactta tagagatggt tcaatatata caggtgaatt taaatacgga ttaaaaaatg
      601 gtttgggaaa attaacatat cccaataatg acatttataa gggatatttt ttagataata
      661 agaaaaatgg taagggtgta ttatataaaa atgatggaaa taaacaagca ggaatatggg
      721 aagatgatga acaatgtaaa agtttagatt ttaaagattt aaataataat aaaataaata
      781 atgtttatca aaatagatta aataacaata aattaaaagt ttaattattc taataaaaaa
      841 atctttattt atgatggaat ataggatata tctatgttgt aatttgtaaa atcttcctat
      901 atcatataaa attaagtatt gattagtcta ttacataaat atagaatata aatatatcca
      961 tttaataata aggagaagag atatgaaaga gtatgtagtt gtggatttag aaactacagg
     1021 attagatcca tataaaggtt gtgaaataat agaaatagga ataacagaaa taaaaaatga
     1081 acaaattgta aaaaactatt caaggttgat aaaacctaag gggataattt cttcatttat
     1141 aacagaattg acacatataa gtaatgaaat ggtagaaaat gaagaaccac tagaattagt
     1201 tttacctaga tttagaaaat acataggtga tagaactata attgcacaca atgcgaaatt
     1261 tgatttaaaa tttttaaatt actatcttag aatgttagat ttagaaccaa taaacaatta
     1321 tatatgtaca gtagagttat taaaaaaatg taaaagttat aaaggaaaaa acaagaaact
     1381 tgaaacagct tgtgcttatt acaacataga gaatataaat gctcatagag ctgacagtga
     1441 tacattagca actgcaaaac tatttttgaa aataaaagat gaatattaat tataaatata
     1501 gagaaaatta gtgaaaatat cctttataaa attgaccctt aagcttacaa ttttgttctt
     1561 tgatttattt tgtagaacaa tgtataatgt accttatatg aagaagggag actaaaatgg
     1621 gttattacaa tgataatgat aataataaaa taagacgtaa aaaaattagt aacaacaaag
     1681 atagtaataa aaaatctaaa cattcttata cttcggcaaa taaaaaaaat aaaagtccta
     1741 atacaagttc cagtaaaaga atgagtgaaa aagtagattc acagaatctt ataaaaagag
     1801 atagagatag ttttacaaga gataatgtaa gacctttaaa tacagattac aatcaaagag
     1861 aaagaagaag agtcgagtat aaacaaagag agaaaaaatt aaaaagtaaa aaaagaaaaa
     1921 aacaaaaaag gattagaata gttagggcat tattatctat tatgttagtt gcactaatta
     1981 tattttcagt ggcaggtgca atatttacag tttcagctat aaaagggtca ccagaagtta
     2041 ctaaaaagtt aattaggtct aaatatataa gtagtgaggt tgtaagtata aaacaaatgc
     2101 caaatgattt aaaacatgca atagtttcca tagaagatga gagattttat aagcataaag
     2161 gagtagatgt tatttctcta gttaggtcag tattaaacaa tgtgtttaca gatacaacac
     2221 aaggtgcaag tactatagat atgcaagttt caaaaaacct gcttactagt aatgataaaa
     2281 ctatgaagcg gaaaataagg gatatgtata atgctaagca gatgaataag attatgacta
     2341 aggatgaaat tcttgaagca tatttaaata atatgtatct aggtaaaaat gcatatggag
     2401 ctgcaaaggg agctgaagtc tacttcggta aaaaagttag tgaattaaat ttagctgagt
     2461 gtgctatgtt agctggtatg acaaataatc ctgcaaggta tattgaccat ggagaggcta
     2521 agaagagaca agtgattgtg ttgtataaga tgcatcagct tggttatata actgataagg
     2581 agtatagaat agcaaaagct gatgacactc catttaaatc agaaattgat taaaattata
     2641 agaatatagt attttcaata gtttaaacaa acgaaaatgt aataaaaaag gtgatttgcc
     2701 acccatttgc caccataatt ataatttaat agcaaattgt tttaatgtac aaatccgata
     2761 atataatatt gttttaaata ctacattatg ctaaaattta attgtatttt gtaaattaaa
     2821 aaacttataa cttagttttc tagggttccg cattttataa tggtcaggtc caagaggaaa
     2881 cgcacaattg ttgtgtacac ggaaggataa aagcctggga gatatttaat aatatttcta
     2941 taggcttttt tatttttaca tattttaaat ttgaggaggt tttctaatga aaaaatttgt
     3001 tattgaggat gcattttgga atctatttcc tagtgcaaaa attggagttg ttatttgtaa
     3061 tgatatagat aactctataa aagatgaaga ctataagaaa ataattttga agggagaaga
     3121 agaagcatta aaatacttgg aggactcaga atttagcaac aaccaagtga ttaaagtatg
     3181 gagagaagca tttaaaaaat ttaaaacgaa aaaaggagca aggtcttcta ttgaagcatt
     3241 attaaaaaga gttcataatg gaaataatat aggaactatt aaccctttag tggatatcta
     3301 caattttatc tcattaaaat atgcattacc ttgtggaggg gaggatatag ataaatttat
     3361 aggagatatt aggctaacta gagcaattgg taatgaggag tttatcccac tagggacaga
     3421 tgaaaattca tttccatatg taggagaaat aatttataaa gatgatggtg gagcaatttg
     3481 tagatgttgg aattggcgtg aagcagtgag aacaatgctt actgaagata ctaacaatgc
     3541 ctttttatgc attgaattag tagatgaaag tagatttaaa gaatttgaaa atgctttaaa
     3601 tgaattagct aaaatagttc aagaaaaatt gggtgggaat tgtaaaattt caattttaga
     3661 tattgataat aaagaggtat cagtggatta atatggaggt gctgttgtaa tggactattc
     3721 tagttataaa gatacttata tttagggtaa gaaggtctat tttagatata acgattatca
     3781 aaggatataa tatagaaaat aaaaatgtag aatataaaaa gcctttaaaa taagaaaaaa
     3841 atgtatttta aaggctttta ttatactctt ttttatttgc ataattaaca ataaaaatat
     3901 attgttaaat ataatatttt tattgaaaaa cgatataata taatgtatta tactatatat
     3961 aaaatatttt aaaggagttg gcatgatgtc taataaactt ataaaaaatt tactaagtgg
     4021 tatattacaa attttatttt ttcttttagg attagtaatt gttgcaggag gatttaaaag
     4081 ttttatgtac ctctgtttta gtggagaagc aactctacaa ggcactattt atggaatatt
     4141 aatgtttatt ttaggtgtat catattttat cattataaaa tcattgatag aagtattagg
     4201 tagttctgaa tatagcctat ttgtaaaaga gaatgtaaaa agatttagga ttattggata
     4261 tcttttgctt ttaaactctc taattgagtt tatttcaact tttggtacta ctggaaaagg
     4321 aatgagattt ctagatttag gatttggatt ttattttact gttccagtat ttgtatattt
     4381 tataacttct cttatgagtt ttgtaattgc agatggattt gtaaaggcaa taaagataaa
     4441 agaggataat gatttaacta tttaggtgtg acactttaat atataggaga taaataaccc
     4501 gaaaagagtg attaactagt aatttaaaat aaaatatatt agggtataat acataaacta
     4561 taatgctaaa attaaaagat
//
LOCUS       DAEMHW010000253         4534 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEMHW010000253 DAEMHW010000000
VERSION     DAEMHW010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.255"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            62..355
                     /locus_tag="KPK67_003494"
     CDS             62..355
                     /locus_tag="KPK67_003494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460411.1"
                     /translation="MIDLLIVERKTEDIKFKNPFVYMITFVSCFIFLFTVYNTTNHII
                     INLIDDKQLNSKSKGVEINEFTYQDWQQFCDIKYYGKKGLISMTLKEYIEKYK"
     gene            610..1377
                     /locus_tag="KPK67_003495"
     CDS             610..1377
                     /locus_tag="KPK67_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460412.1"
                     /translation="MKNILSVEKIEKYYGNKDNITKAIDNISFKVDEGEFVGIMGPSG
                     SGKTTLLNCISTIDNVTIGKIIINNNDITRLKSKNLDKFRQNELGFIFQDFNLLDTLT
                     AYENIALALTIKGEKPSKIDSKIRDVATYLEIEKVIDKYPYQMSGGQKQRVASARAIV
                     TNPSLILADEPTGALDSKSARLLLERFESLNQDLKATILMVTHDAFTASYAHRILFIK
                     DGKIFTELVRGADSRKEFFNRIIEVTTLIGGDDNDVF"
     gene            1367..3361
                     /locus_tag="KPK67_003496"
     CDS             1367..3361
                     /locus_tag="KPK67_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460413.1"
                     /translation="MYSKIAIKNVRKSFKDYSIYFLTLTLAICIFYSFNSIESQKALM
                     EMNASTKSHVEMLKNMISYMSVFVSVILCSLILYANNFLVKKRNKELGIYMTLGMEKV
                     KISKILVFETIIVGIVSLVSGLILGLLVSQGLSILLLKLFEFNMSEYNFVISISSIIK
                     TIVYFGIMFLLVMIFNTYIISKYKIIDLLTIGRKTEDIKFKSPFVYTITFILCIISLI
                     IAYSMVLKVGLNPKNPKFIMSIVLGVIGTILFFYSLAGFALYIVKKNKNIYFKGLNIF
                     VVKQINSKINTNFISISIICLMLFLTIGILSTGFGFKSALNAGLKGATPFDASATMIV
                     HEKCKVKNIKESFEKVGIKFNKGDKVAYYDEYINGNKLSDVMQGQYSGRVDFEVAYIK
                     SSQYNEIRKLRGEKSITLSKDEVLITSNATEVVPSIEEYMKNNKIIKIDNKAYNIKNK
                     KVIKDNLRTDFTETNALTIVVNDKLCDSMEISSSIVNINFALDNNEKSQEDFDKRLKS
                     KYSSTSFYEVGTVSGGSRNAMYNDNNGMTSTILFVGIYLGIVFLISSMAVLALQQLSE
                     ASDSIDRYVSLKKLGASKNSISKTIFTQTLMYFSIPVGLALVHSVVGIQVANNFISRL
                     NKPDIGASSLMTVGILMLVYAVYFYTTYQGYKNIVIKKLN"
     gene            3438..4109
                     /locus_tag="KPK67_003497"
     CDS             3438..4109
                     /locus_tag="KPK67_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005210744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2460414.1"
                     /translation="MEKIIIVEDEEVIRDELKSFLERYGYEVMAPTEFDNIVEFILSE
                     KPNLVLLDINLPIYDGYYICRVLRKESNIPIIIVTSRNTDMDELMSMNLGADDFVTKP
                     YKTQILLARIVALLKRSGLNSNVNNTLIFKGIELNLFNATLRYKDKEIELTKNEFKIL
                     SYLVENQGSIVSRDSFMEYLWHTNWFVDDRTLTVNITRLRKKLDEIGIENLIETRRGL
                     GYIIS"
     gene            4106..>4534
                     /locus_tag="KPK67_003498"
     CDS             4106..>4534
                     /locus_tag="KPK67_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2460415.1"
                     /translation="MRLREYIGDKVVFLGTNLIIFLIASGIMLYSKINVVLIFMLFCI
                     WFLPLFTYMLIDYLQYKKYFNSIDNILDALDKKYLLPEVIDDVNFVTGEKINDILKVI
                     SRNMHENIKYYNDNQEEYKEYIETWVHEIKTPIASSKLIIE"
BASE COUNT         1884 a          420 c          708 g         1522 t
ORIGIN      
        1 ttttggaata atatttttac ttgtaatgat atttaatatc tatttaattt ctaaatacaa
       61 aatgatagat ttattaattg tggaaagaaa aacagaggat ataaaattta aaaatccatt
      121 tgtatatatg attacatttg tatcatgctt tattttctta tttactgtat ataacactac
      181 aaatcatatc ataattaatc taatagatga taaacaatta aactccaagt ctaaaggtgt
      241 agaaataaat gaatttacat atcaagattg gcaacaattt tgtgatataa agtattatgg
      301 aaagaaaggt ttaatatcaa tgactttaaa agaatatata gaaaaataca agtagaataa
      361 aataaatttt agatggaagg cattgccctc tatatttttg tgttttctag aattgagtaa
      421 attttgttac tctagcaaag tataaaagaa agaatgctgg tcttgaatat tttatattag
      481 taattattag ggaataatat ataattacta caaaaatgaa acattaatga aatgtaaatc
      541 aatttaaaaa aattaaataa aaaattacaa tagagatata agaaaattaa tttaaaggag
      601 aaataggaca tgaagaatat attaagtgtt gaaaaaatag aaaaatatta tggaaacaaa
      661 gataatatta caaaagcaat agataatata agctttaaag ttgatgaagg agaatttgtt
      721 gggataatgg gtccaagtgg tagtgggaag acaacacttc ttaactgtat atcaacaata
      781 gacaatgtaa caataggtaa aataataata aataataatg atattacaag attaaaatct
      841 aaaaacttag ataagttcag acaaaatgaa ttaggattta tattccaaga ttttaatctt
      901 ttagatactt taacagctta tgaaaatata gcattggcat taacaattaa aggagaaaaa
      961 ccatctaaaa ttgattcaaa aataagagat gtagctacat acttagaaat agaaaaagtg
     1021 atagataaat atccttatca aatgtcagga ggacaaaagc aaagagtagc atcagctaga
     1081 gcaatagtta ctaatccatc attgattttg gcagatgaac ctacaggagc acttgactca
     1141 aaatcagcaa gattattact tgagaggttt gaaagtctaa atcaagactt aaaagcaaca
     1201 attttaatgg taactcatga tgcttttaca gctagttatg cccatagaat cctatttata
     1261 aaagacggaa aaatatttac agaattagta agaggagctg attcaagaaa agagtttttt
     1321 aatagaataa ttgaagtaac aactctaata ggaggagatg ataacgatgt attctaaaat
     1381 agcaattaaa aatgttagaa aaagctttaa ggattatagc atatattttt tgactttaac
     1441 attagcaatt tgtattttct atagttttaa ctctatagaa tctcaaaaag ctttaatgga
     1501 aatgaatgct tcaacaaaat ctcatgtaga aatgcttaag aatatgattt catacatgtc
     1561 tgtatttgta tcagttattt tatgcagttt aattctttat gctaataact tcttagtcaa
     1621 aaaaaggaat aaggaacttg gaatttatat gactttaggg atggaaaaag ttaaaatatc
     1681 gaaaatatta gtttttgaaa ctataatagt tggaatagtt tctttagtaa gtggactaat
     1741 acttgggtta ttagtatctc aagggttatc aatattatta ttaaagttat ttgaatttaa
     1801 tatgagtgaa tacaattttg taatatcgat atcatcaatt attaaaacta ttgtctattt
     1861 tggaataatg tttttacttg tgatgatatt caatacttat ataatttcta aatataaaat
     1921 aatagattta ttaacaatag gaagaaaaac agaggatata aaatttaaaa gtccatttgt
     1981 atatacaatt acatttatat tatgcattat ttccttaatt attgcatata gcatggtttt
     2041 aaaagttgga ttaaatccta aaaatcctaa gtttattatg tcaatagtat taggtgtaat
     2101 tggaactata ttattcttct atagtttagc aggctttgca ttatatattg ttaagaagaa
     2161 taagaacatt tactttaagg gattaaacat atttgtagta aagcaaataa atagtaagat
     2221 taatacaaac tttatatcaa tatctattat ttgtttaatg ttatttttaa caataggaat
     2281 actatcaaca gggtttggtt ttaaatcagc tttaaatgca ggactaaaag gtgcaactcc
     2341 atttgatgca agtgcaacca tgattgtaca tgaaaaatgt aaagtgaaga atataaaaga
     2401 atcttttgaa aaggttggaa ttaagtttaa taaaggtgat aaagttgctt actatgatga
     2461 atatataaat ggtaataaac tttcagatgt tatgcaagga caatattctg gtagagtgga
     2521 ctttgaagta gcatatataa aatcttcaca gtataatgaa ataagaaagt tacgaggtga
     2581 aaagtcaata actttatcaa aagatgaagt attaataaca tctaatgcta cagaggtagt
     2641 accttcgata gaagaataca tgaaaaataa taaaattata aaaatagata ataaagcata
     2701 taatataaaa aataaaaaag taataaaaga taatttaaga actgatttta cagaaacaaa
     2761 tgctcttact atagttgtaa atgacaaatt gtgtgatagc atggagatat cttcaagtat
     2821 agtaaatata aacttcgctt tagataataa tgaaaagtca caagaagatt ttgataaaag
     2881 gctaaaatca aagtatagtt ctacaagttt ttatgaagtt ggaactgtat caggtggatc
     2941 aagaaatgct atgtataatg ataataatgg tatgacttct acaatactat ttgttggaat
     3001 ctatttagga atagtatttt taataagtag tatggcagtc cttgcacttc agcaattaag
     3061 tgaggctagt gatagtatag atagatatgt atccttaaaa aaactaggag caagtaaaaa
     3121 tagtataagt aaaactatat ttacacaaac tctaatgtac tttagcatac ctgttggact
     3181 agctttagtt cactcagtag taggaataca agtggctaat aattttatat caaggcttaa
     3241 taagccagat ataggagctt catcacttat gacagtagga attttaatgt tagtatatgc
     3301 agtgtatttt tatacaactt atcaagggta taaaaatata gttataaaaa agcttaatta
     3361 atacaactaa ctaaaatctg tttacagtaa aaatcatact gtaaatagat tttttgaaaa
     3421 taaagggtga aaaattaatg gaaaaaataa ttatagtgga agatgaagaa gtaatcagag
     3481 atgaattaaa aagcttttta gagaggtatg gatatgaagt tatggctcct actgaatttg
     3541 ataatatagt agagtttata ttaagtgaaa aacctaattt agtattactt gatataaact
     3601 tgccaatata tgatggatat tatatatgta gagtattacg taaagaatcg aatataccaa
     3661 taataattgt aacaagtaga aacactgata tggatgaact aatgagcatg aatttaggag
     3721 cagatgattt tgtgacaaag ccatataaaa cacaaatact tttagcaagg attgttgcat
     3781 tgttaaaaag aagtggatta aattcgaatg taaataatac tttaatattt aagggtattg
     3841 aattaaattt atttaatgca acactcagat ataaagataa agaaatagag ttaactaaaa
     3901 atgaatttaa aatactatct tatttagtgg agaatcaagg ttcaatagta tctagagatt
     3961 catttatgga gtatctatgg cacacaaatt ggtttgtaga cgatagaact cttactgtca
     4021 atataacaag gctacgtaaa aagctagatg aaataggcat agaaaatttg atagagacaa
     4081 gacgtggtct agggtatata atatcatgag attaagagag tatataggtg ataaagttgt
     4141 ttttctaggt actaatttaa ttatattttt aattgcatca ggtatcatgc tttatagtaa
     4201 aataaatgtc gttttaatat ttatgttatt ttgtatatgg tttttaccat tatttactta
     4261 tatgttaatt gattatttac aatataagaa gtactttaat tctatagata atattttaga
     4321 tgctttagat aaaaaatatt tgttgccaga agttattgat gatgtaaatt ttgtaactgg
     4381 agaaaaaata aatgatattt taaaagtaat aagtagaaac atgcacgaaa atattaaata
     4441 ttataatgat aatcaagaag aatataaaga atatattgaa acgtgggttc atgaaataaa
     4501 aacgcctata gcatctagta aacttattat agaa
//
LOCUS       DAEMHW010000254         4528 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEMHW010000254 DAEMHW010000000
VERSION     DAEMHW010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.256"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            98..1009
                     /locus_tag="KPK67_003499"
     CDS             98..1009
                     /locus_tag="KPK67_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2460416.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKELSLKAKGLLSQMLSLPEDWD
                     YTLAGLSLINRESIDAIRTAVWELEKAGYITRRQGRDEKGKMTAIEYTIYEQPQPPAL
                     DCPVLENPTADNPILENPTPDNPTSENPTQLNKELLKTNLPKKEKLNTDVSSTHSIPF
                     HSLNSSPLEDAAQPPERKRKETTDAYRVYEEIIKDNIEYEHFIKHTNIDRERLDEIVS
                     LILETVCTKRKTIRIAGDDYPAELVKAKFMKLNSSHIEFVFDCMKENTTKIRNIKQYL
                     KAVLFNAPNTIDSYYTALVNHDMYGGY"
     gene            1029..1247
                     /locus_tag="KPK67_003500"
     CDS             1029..1247
                     /locus_tag="KPK67_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBG2460417.1"
                     /translation="MAQKTGALIFDETADRYDIRFDLNDYYGGLHCGDCLEVFVRGKW
                     KPTRMEYGDNWYLVGVRASDLNGLRVRI"
     gene            1330..1806
                     /locus_tag="KPK67_003501"
     CDS             1330..1806
                     /locus_tag="KPK67_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBG2460418.1"
                     /translation="MQDEVNEKVVSLAIKTSKLTAEVLQKAMKALLAKGKGQLTKAPH
                     GKITMRQLMKPGEKVSNIEITDQNIKAFDPIARKNGLDYNVKKIENGKPPTYLVSFKG
                     KDIDVMTEAFREFSAKKLGRDKKPSIRKLLSTLKDKAAALNAQRDKVKKKDREVSL"
     gene            1803..3590
                     /locus_tag="KPK67_003502"
     CDS             1803..3590
                     /locus_tag="KPK67_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007037036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBG2460419.1"
                     /translation="MKPELKKLLILNAPYLLFVYLFDKIGQAVRLSPGADLSGKVLSL
                     ADGFSAAFASPLPSLAPMDLLIGIAGAVLIRLMVYFKGKNAKKYRKGIEYGSARWGNA
                     EDIKPYTDPVFQNNVLLTQTERLTMNSRPKQPKYARNKNILVIGGSGSGKTRFFVKPN
                     LMQMHSSYVVTDPKGTVLVECGKLLQRGGYRIKVLNTINFKKSMRYNPFAYIRSEKDI
                     LKLVNTLIANTKGDGEKAGEDFWVKSERLFYCALIGYIWYEAPEEEKNFTTLLEMINA
                     SEAREDDPEFQSPVDLMFERLEEKDPEHFAVRQYKKFLLSAGKTRSSILISCGARLAP
                     FDIKELRDLMETDEMELDTIGDRKTALFVIISDTDDTFNFVVSILYTQLFNLLCDKAD
                     DEYGGRLPVHVRCLLDEFANIGQIPKFEKLIATIRSREISASIILQSQSQLKAIYKDN
                     ADTIVGNCDTTLFLGGKEKTTLKEMSEILGKETIDSFNTSENRGREVSHGLNYQKLGK
                     QLMTEDEIAVMDGGKCILQLRGVRPFFSDKYDITKHPNYKYLSDYDKKNTFDMEKHLR
                     RRPALVKPGEVFDYYEISESDLQEDTDHE"
     gene            3583..3750
                     /locus_tag="KPK67_003503"
     CDS             3583..3750
                     /locus_tag="KPK67_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460420.1"
                     /translation="MNRRIRKKKDKKITEAINGLVSIAERREQQRQAAIRQFVKRCEA
                     LYEQQRKERRN"
     gene            3950..4165
                     /locus_tag="KPK67_003504"
     CDS             3950..4165
                     /locus_tag="KPK67_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBG2460421.1"
                     /translation="MEFFNSAIGVLQTLVIALGAGLGIWGVINLLEGYGNDNPGAKSQ
                     GIKQLMAGGGVALIGTILVPLLSGLFG"
     gene            4182..>4528
                     /locus_tag="KPK67_003505"
     CDS             4182..>4528
                     /locus_tag="KPK67_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460422.1"
                     /translation="MQSILDAINEWIKEILIGAINGNLSTMFGDVNEKVGTIANEVGQ
                     TPQGWNANIFSMIQTLSENVIVPIAGLVITYVLCYELISMVTEKNNMHDIETFMFFKW
                     IFKAFVAVFIVTNT"
BASE COUNT         1367 a         1046 c         1110 g         1005 t
ORIGIN      
        1 ttaggacgtg aaaatgagaa aggcaagggt tgataccttt tccctcaaaa ccgcgtttct
       61 actgcgtaac aaatccaacc aaaggagtga tgaagctatg gcagttttcc gcgtggaaaa
      121 gaataaaggt tatacggtta tgagcaacca ccatttacga aacaaggagc tttccttaaa
      181 agcaaaggga ttgttgtcgc aaatgctttc acttcccgaa gattgggact atacccttgc
      241 agggctgtcc cttatcaacc gggaaagtat cgacgctatc cgcaccgctg tatgggagct
      301 tgaaaaagcc ggatatatca caaggcggca gggacgcgac gagaaaggca aaatgaccgc
      361 tatcgagtac accatttacg agcagccaca gcccccggca ttggattgtc cggtattgga
      421 aaatccaaca gcggataacc cgatattgga aaatccgaca ccggataacc cgacgtcgga
      481 aaatccaacg caattaaata aagaactatt aaagactaac ttaccaaaaa aagaaaaatt
      541 aaatacagat gtatcaagta cccattccat tcctttccat tccctaaatt cctctccctt
      601 agaggacgcg gcacagccgc cggaacggaa gcgaaaggaa acgacagacg cataccgagt
      661 gtatgaggaa atcatcaagg acaatatcga gtatgagcat tttatcaagc ataccaacat
      721 tgacagggaa cgcttggacg agattgtgtc ccttatcctt gaaactgtct gcactaaacg
      781 aaaaacaatc cgtatcgccg gggacgatta tccggcagag cttgtcaaag caaagtttat
      841 gaagctcaac agcagccaca ttgaatttgt ctttgactgc atgaaagaga acaccacgaa
      901 aatccgcaac atcaagcaat atctgaaagc ggtacttttc aacgccccca acaccataga
      961 cagctattac accgcccttg tcaaccacga tatgtacggc ggctattaaa aaacaggagg
     1021 attttaatat ggcacagaaa acaggagctt tgatttttga tgaaacggca gaccgttatg
     1081 acattcgctt tgaccttaac gactactacg gaggattgca ttgtggggat tgccttgaag
     1141 tgtttgtacg cggcaaatgg aagccgaccc gcatggagta cggggacaac tggtatcttg
     1201 tgggtgtgag ggcttcggat ttgaacgggc tgcgggtgcg tatctaaagg cggcatgaaa
     1261 aatgtacgcc ttatttttat gccacgaagc ggaataccac cctaacaaaa ccacgaaagg
     1321 agggatttca tgcaagatga agtcaacgaa aaagttgtgt ctttagcaat caaaacatct
     1381 aagctgaccg ccgaagtgct gcaaaaggct atgaaagccc tgcttgccaa aggcaaaggg
     1441 cagctaacca aagccccaca tgggaaaatc actatgcggc agcttatgaa gccgggagaa
     1501 aaagtttcca acattgagat taccgaccag aatatcaaag cctttgaccc tatcgcaagg
     1561 aaaaacgggc ttgattacaa cgtcaagaag attgagaacg gcaagccgcc gacctatctt
     1621 gtgtctttta agggcaagga tattgatgtt atgaccgaag cgttccggga atttagcgca
     1681 aagaaattag gcagggataa aaagccctct atccgcaagc tgctttctac tctgaaagat
     1741 aaggcggcag ctctgaacgc acagagggac aaagtgaaga aaaaggacag ggaggtatcg
     1801 ctatgaagcc ggaactcaaa aagctgctta tcctaaatgc cccctatctg ctctttgtct
     1861 atctctttga caaaatcgga caggcggtgc ggctctctcc gggggctgac ctttccggca
     1921 aggtgctttc tcttgcagac ggcttttccg ctgcctttgc aagcccgctt ccgagccttg
     1981 cccctatgga tttgcttatc ggtattgcgg gggctgtcct tatccggctt atggtgtact
     2041 tcaaaggcaa gaacgcaaag aaatatcgaa aaggtatcga atacggttct gcccgttggg
     2101 gcaacgccga agatataaag ccctacaccg acccggtatt tcaaaacaac gtgctgctga
     2161 cacagacgga acggcttacc atgaacagcc gcccgaagca gccgaagtat gcaaggaata
     2221 aaaatatcct tgttatcggg ggaagcggca gcggcaagac gagatttttt gtgaagccca
     2281 acttaatgca aatgcactca agctacgttg taaccgaccc gaaaggtaca gttttagtcg
     2341 agtgcgggaa gctcttacag cgcggcgggt atcggataaa ggtgctgaac acgataaatt
     2401 ttaagaaatc catgcgttac aatccctttg cctatatccg cagcgaaaaa gacattttga
     2461 aactggtaaa taccttgatt gccaacacca aaggggacgg ggaaaaagcc ggggaggatt
     2521 tttgggtaaa atcggaacgg ctcttttact gcgcccttat cggctacatt tggtacgaag
     2581 ccccggagga agaaaagaac ttcacgacgc tgcttgaaat gataaatgcc agtgaagccc
     2641 gcgaggacga cccggaattt cagtcccccg ttgacctcat gtttgaacgg ttggaggaaa
     2701 aagacccaga acactttgcc gtccggcagt ataagaaatt cctgttatct gcgggaaaga
     2761 cacgaagctc tatcctcatt tcctgcggtg cgaggcttgc cccttttgac attaaggagc
     2821 tgcgcgacct tatggaaacc gacgaaatgg agcttgacac cataggcgac cgcaagaccg
     2881 ccctgtttgt tatcatcagc gacaccgacg atacctttaa ctttgtggta tcaatccttt
     2941 acacacagct tttcaatctc ctttgcgaca aggcagatga tgaatacggc ggcaggctgc
     3001 ccgtccatgt gcgttgtctg ttagacgagt ttgcaaatat cgggcagata ccgaagtttg
     3061 aaaaactcat agccaccata cgaagccggg aaatctccgc gtcgattatc ttgcagtcgc
     3121 aaagccagtt aaaggcaatc tataaggaca acgccgatac catagtcggc aactgcgaca
     3181 ccaccctttt cttgggcggc aaggaaaaaa caaccctcaa agaaatgtcg gaaatcttgg
     3241 ggaaagaaac cattgacagc ttcaacactt ccgagaaccg ggggcgtgag gtatcgcatg
     3301 ggctgaacta tcagaagtta ggcaagcagc ttatgacgga agatgaaata gcagtcatgg
     3361 acggcgggaa atgtatcttg cagcttagag gggtgcgccc gttcttttcc gataagtacg
     3421 acattacaaa gcaccccaac tataaatatc tttccgacta tgacaagaaa aatacttttg
     3481 atatggagaa gcatttaagg cgcagacccg cccttgtgaa gccgggcgaa gtatttgact
     3541 actacgaaat cagcgaaagc gatttgcagg aggacaccga ccatgaatag acgtatcaga
     3601 aagaaaaagg acaagaaaat cacagaagcc ataaacgggc ttgtttcgat tgctgaacga
     3661 cgggaacagc agcggcaggc tgctatccgg caatttgtga aacggtgtga agccctgtat
     3721 gagcagcagc gaaaagaaag gaggaattga cgcagtaaca aagaaaaaga caacttgcat
     3781 gatacgcgcc cctacaaaaa ttcctatcgc ccacactgac aactgaatac cgccgcgcgg
     3841 cagacattta ggcagcgcgg ggacttatga ccgcggcagt ttgaaaactg accgccgttt
     3901 tttatgccct tttacggggc aagccgcatt tgcggcagaa aggagtttta tggaattttt
     3961 caacagcgca atcggcgtat tacagacttt ggttatcgct cttggagcag gacttggcat
     4021 ttggggcgtt atcaatctct tagagggata tggaaacgac aatccgggcg caaaatcaca
     4081 gggcattaag cagctcatgg cgggcggcgg cgttgccctt atcggcacta tccttgtacc
     4141 tctgctttcc ggcttgttcg gttaagcgcg ggtagctgcc tatgcagagc atacttgacg
     4201 cgattaacga atggataaag gaaatcctta tcggagcgat aaacggtaat ctgtcaacta
     4261 tgttcgggga cgtaaacgaa aaagtcggga ctatcgcaaa cgaggtagga cagaccccgc
     4321 agggttggaa cgcaaacatt ttcagtatga tacagaccct ttctgaaaat gtaatcgtcc
     4381 cgattgcggg gcttgtcatt acctatgtcc tatgctacga gctaatcagc atggtaacgg
     4441 aaaagaacaa tatgcacgac atagaaacat tcatgttctt taagtggata ttcaaagcct
     4501 ttgttgcggt gtttatcgta accaacac
//
LOCUS       DAEMHW010000255         4501 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEMHW010000255 DAEMHW010000000
VERSION     DAEMHW010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.257"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            170..517
                     /locus_tag="KPK67_003506"
     CDS             170..517
                     /locus_tag="KPK67_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG2460423.1"
                     /translation="MHENKKNLLDGSLAQYNDAAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(667..1716)
                     /locus_tag="KPK67_003507"
     CDS             complement(667..1716)
                     /locus_tag="KPK67_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2460424.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKGESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            2102..2836
                     /locus_tag="KPK67_003508"
     CDS             2102..2836
                     /locus_tag="KPK67_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2460425.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     SKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGTNLHWPVDGYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(2979..3470)
                     /locus_tag="KPK67_003509"
     CDS             complement(2979..3470)
                     /locus_tag="KPK67_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG2460426.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            3819..4202
                     /locus_tag="KPK67_003510"
     CDS             3819..4202
                     /locus_tag="KPK67_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460427.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
BASE COUNT         1660 a          567 c          629 g         1645 t
ORIGIN      
        1 gttaattttt tatatcttat aaggattgta tttttctaac tataagaata gtattatact
       61 tatatttaaa aataaaagaa ttttattcct attgtgtaaa gtgagaatac tattttctta
      121 tattggtata ataatagtaa atctaatata gaaaagaggt atttctagta tgcatgaaaa
      181 taaaaaaaat ctattagacg gaagcttagc tcaatataat gatgcagcag ttccaaaaat
      241 accagtattt gctggtaacg atataacttc agaagtttat tattttaaac ctaatcaagt
      301 tcttaatgct catagacacc ctaatggtga acaaatattt gtgttcctaa aaggagaagg
      361 taaaatgaaa cttggtgagc atgaatgtga tgtaaaaaac ggcgatactg tatttgtacc
      421 aacaggagag tggcatgaaa ttactaatgg aagcaatgaa gaaatggtag ccgttcaaat
      481 tacaaaaatt aatgctggag cagaatacag aggatagata tataagtaca gttagtactt
      541 taatatctat tttattatat aaactttaat ctcaaatata aaaagagtct tttaaaaggc
      601 tctttttata tttttgttaa ttttcattat aaaaacaaga aaatatctag ctaaaactaa
      661 tatattttat ttaatatttt ttatagcatt gtttgcaaac tcagtattta cgatatcttt
      721 ataaggtgct tccttatcta gttctttagc ttctttcata acagtcatca atttcttcaa
      781 accttcctct tttaatacag gattttgaga ccatgcatct attgatttat atctatctac
      841 aactgttgtt aaatcttcta aagatatatc ttcaaagaaa ggttgcatag ccttagctac
      901 ttctttgcta gatgcttgtt gaatccataa ttgaccttta taaacagcgt ttgtaaatct
      961 ttgaattaaa tctttattgt ttttcatgta tgatctcgta gtagagtatg ctgtaaaagg
     1021 tatttctcca gaatcttttc ctatagatgt aactatatat ccactacctt ctttttccat
     1081 tgctgttgct gtaggttcaa atgctgttgt aaagtcaccc tctccacctg caaaagctcc
     1141 agccattaca ccaaattgta catcagttct tacattaaca ttgcctgctt ttagattttc
     1201 tccaatagta agaccttttt cttttagaac atattcaaaa gtcattagtg gaactccacc
     1261 ttttctacca cctaatattt cggttccttt taaatcattg taagagaaat taggcatttt
     1321 tttcctagca actagaaaac taccatctct ttttgtcaat tgagcaaagt tcactgcata
     1381 atcgtttttg ttttgattat atacatatat agatgcttca ggacccatta aacctacgtt
     1441 tgcttcacct gaaatcaaag ctgccatagt tttatctgca ccttgggtat ttataaggtc
     1501 aatctttata ccttcatctt caaagaatcc tttcgtaata gcagcatact gtggagcata
     1561 aaaaacagag tgagtaactt ctgctatagt tactttatcc agatttttag acgaagaaga
     1621 ttcacctttt ttgtcattag agttaaaatc acatccagtt agacatattg tacttacaat
     1681 gatagagctt gctattgcga ctattttttt aaacataata aatacctccc ataagacata
     1741 cgaatagtat atagtattaa aaatttattg tagttgttac aaaaatgtat ctatgcttat
     1801 aatatttcta caaaaactta ataataattt tcagttttat gtttagtttt tttagtaatg
     1861 tgtaatattg gataaattat attgattatt tataaactaa gtaataaaat taaaataata
     1921 tcactttttt gtctattata tataaggaaa aattttttaa taatataaat catattcatt
     1981 tatattatta aaaaatttgt ttatagaaac ttttattatt ttatataaaa gtgataaaaa
     2041 cttatatttt gtgtttagta gagtgttgct aagtgaatat atttaaaatg gaggaaactt
     2101 tatgaaacaa aataaatatg atgatgatag attttttaat aaatacagta aaatggaacg
     2161 ttctacaaat ggattagcag gtgctggaga atggcatgtt ttaaaaaaaa tgttacctga
     2221 tttcaaagga aaaagagttt tagatttagg atgtggattt ggatggcatt gtcaatatgc
     2281 agttgagaat ggagcaattt ctgctgttgg agttgatatt tcagaaaaaa tgttaaaaga
     2341 agctagaaac aaaacgcaac ttgataatat aaattacata tgtatgccta ttgaagatat
     2401 taatttttca aaagatagtt ttgatgtagt tattagttcc ttggcatttc attatattca
     2461 atcatttgaa gatatatgta agaatataag taattgtctt tcaaataaag gtaattttgt
     2521 attttctgtt gaacatccta tatttactgc atatggaagt caagagtggt actgtgataa
     2581 agatggcact aatctacact ggccagtaga tggatatttt gaagaaggag tacgtaagtc
     2641 aaattttcta ggagaagagg ttataaaata tcataaaaca ttaacaactt atttaaatac
     2701 gttaatgaaa acaggatttg aaatattaga aattattgaa cctcaaccag aagaaaaact
     2761 gttagataca attcctgata tgaaagatga gctacgtaga cctatgatgt tacttgtatc
     2821 tgcaaaaaaa agataagtaa atataaataa tcattattgt agaaaaaaag ttgtagaaaa
     2881 taattacact aatggagatg attataccag tggtattaat tacaccagta gtaaggaggc
     2941 aaaagcattc gttgcttttg ccatttttta agttgcaatt atacttctac gtagtatatt
     3001 tcgtttccat tttcttctat aagctttttc atatcttcaa attttaaaaa tacagtagca
     3061 gtattatcat ttggatgtat acctatttta tccattttta ataaatcctt atcaaataca
     3121 acttttacaa cagcctcttt atcatttaat ataccaaaag gagtcacagc tcctttagta
     3181 agtttcaagt atttttgaag tctttcttct gatgcaaaac taagaggttt actatcaatt
     3241 tgttgacgaa tatcttttaa atctgctttt ttgtctttac ctaatacaac taaaaaatgt
     3301 tcttttcctt tagcatcacg aagaaatagg ttttttacaa caaattccat atgtaatgtt
     3361 tttaattctt ccatttcttc aattgtataa actggtggat gatttacagc ctcataaggt
     3421 atgcttaact catctaattt tttatatact tcctgttgtt gattgttcat gatttcctcc
     3481 cattagaaat ttattttata tataaataat attatcacag aaaactttaa aagaaaacgt
     3541 tttatataat atagacaata gattatattt ttataaagaa gcaagaatac tcaataaaac
     3601 ttaaagaaat taaacaattt ctaaaaatac cttgaaaata gaaccttatt tgccatttat
     3661 aaattatata aaaagggatt atataaagtc atatatgaaa aaactatatt atactatctt
     3721 ttctttttgg aataaactat actttaaata tagatattta atttaaataa attttacagt
     3781 attaatagta aattatctta gaaaggaatg tgctttatat gtctacagac aaagttgttg
     3841 gaatcataga tgaagaaacg gcagaacttg caggaattga atatactggt aaaatttatg
     3901 cttcttctgg tgttataaaa catataaaga aaaaacatag atgtcaactg agtaaggata
     3961 tttttaatga tataatagat actataaaaa tggtattaaa gtcacctgaa tatataggaa
     4021 gtcatcctaa aaaacctgga aaaagtgtgg agtttataaa aaaaattgat gattatattt
     4081 tagtagctac agaattagat acaaaaaaag gatatttata tgtatcttca atgtatgcta
     4141 taaaagaagc aaaattagaa agtagagtaa acagtggaag agtaaaacaa tatgatggtt
     4201 aacataatat tattaatttt ccctaaaata ttgaaaaata ttattatata agttataata
     4261 ttaatataca cgtagatatt tattagttag atttgaggtt ggaaaaggca cccaatacgc
     4321 tactatagag tagttaatag agatgcagga tacgccgccc tgccaaatct aattaaatta
     4381 aaaagagagg attaattcct ctcttattgt ttttttgaga tactaattta atctataata
     4441 gaaagattga aggtcgttta tacaaaaaaa ttttcagtta tatacattaa actaattaaa
     4501 t
//
LOCUS       DAEMHW010000256         4496 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEMHW010000256 DAEMHW010000000
VERSION     DAEMHW010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.258"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            306..1925
                     /locus_tag="KPK67_003511"
     CDS             306..1925
                     /locus_tag="KPK67_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG2460428.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPIDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2299..2493
                     /locus_tag="KPK67_003512"
     CDS             2299..2493
                     /locus_tag="KPK67_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2460429.1"
                     /translation="MINYMVEIIYSEKISEIENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(3052..3375)
                     /locus_tag="KPK67_003513"
     CDS             complement(3052..3375)
                     /locus_tag="KPK67_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460430.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(3420..3674)
                     /locus_tag="KPK67_003514"
     CDS             complement(3420..3674)
                     /locus_tag="KPK67_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460431.1"
                     /translation="MENKDFKLTVCIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            complement(3938..4219)
                     /locus_tag="KPK67_003515"
     CDS             complement(3938..4219)
                     /locus_tag="KPK67_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460432.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT         1638 a          578 c          511 g         1769 t
ORIGIN      
        1 atatcattct aaaaagatgt tattttttta catatttttt acaattatag ttaattcttt
       61 gttaaattga ttgtacataa aatactaaaa atctaagttc aagtatttaa attgctatat
      121 atcataaatt ttctaaaatt aacaacaact taaaaattta acattctctt aacgtatact
      181 taacaaaaca ttaatatttt tattgacaaa atttgttatt ctatattatg gaaacaataa
      241 attttagtta ataaagttta taattcagat aaaaaaatta ttaatttctt aagggaggaa
      301 atatcgtgaa tatagtaatt agccttatcg gtggacttgg actttttctt tacggaatga
      361 gtttaatggg agaaggttta caaaaatcag caggagacaa attaaaaaaa attattgaac
      421 tcttaacaag taatgttgtt atgggtgtac tggtgggtac agtcgttaca ggtattatac
      481 aaagttcaag tgctactact gttatggttg taggatttgt aaatgcaggt ataatgaatc
      541 taagtcaagc tattggtgta ataatggggg caaatatagg tactacagtt actgctcaat
      601 tagtttcatt taatcttgaa ggaattgcac caattgcttt aggtataggt attttattct
      661 atttatttac atcaaatcaa aaaacaaagc atttatctga gatacttata ggtttcggta
      721 tattatttac aggaatggag tttatgaaag atgcagtagc tccacttgct gaatataaag
      781 cattcactga tgcactttta tattttagta aaaatcctgt attaggtata ttggcaggat
      841 ttgccataac tggtattgtt caaagttcaa gtgcatctat gggtatgtta atagcactag
      901 cttctcaagg aatacttcct ctatcatctg ctttacctat tctttatgga gataatatag
      961 gtacttgtgt tacttcactt ttatcaagtg ttggtgcaag tagaaatgct cgacgtgcag
     1021 ctgtaatgca tttaagtttt aatgtaattg gtacaataat atttatgctt gttttaaata
     1081 aacctatatc agccatagtt actcattttg acccaattga tacagctaga caaatagcaa
     1141 atgcacatac tttatttaat cttacaaatg ttataatatt attaccattt tctaaataca
     1201 tagttaaatt agcaaataga cttataccta ttaaagaaac tgaatcagaa atagtaaata
     1261 atactaagta cttagatgaa aggatgttta gcactccatc tatagcactt ggtaatactg
     1321 tacaagaagt tgtaagaatg ggtcataaag ctacaaactc tttggaacat tcaattgcag
     1381 gatttttaaa taagagcaat gaagatatta ataaagcttt tgagtcagaa aaagttgtca
     1441 ataagttaca gaaagatata ttgaactatt tgttaaaact ttctaaagaa cctcttagag
     1501 atgatgaacg ctttagaaca gatttgcttt ttaatactgt aaatgacatt gaaagagtct
     1561 ctgaccatgc agaaaatata gctgaattgg ccatgagtgt taaggaaatg aacattagtt
     1621 tttctgatag tgcgataaga gaaatatacg aaatatacaa taagacaatt actaatttta
     1681 aggatgcctt aatagtttta gatgttaaag attttgagtt agcaaataaa gttttagaag
     1741 ttgaaaatga agtaaattat ttagaaaaaa cttttagaaa ttcacatatg attagactaa
     1801 ataatggaag ttgtacaatt gatgctggtg tactttatct tgacttactt actaatctag
     1861 aaagaatttc tgaccactca acaaatatag taaaacaagt actaaaactt aaacaaaaaa
     1921 tatagtgatt atttttatat ataatcttaa tcaatgagaa gtaagaagct atagataatg
     1981 cttatatcat ttatggcttc ttatttttta tgtaatactc aaatttttat agtattctct
     2041 tattattaaa caatttctgc ctagaatatt ccatatatat atgacttctt tcataaaact
     2101 tgaactgccc ataactaaag tcacaagtat acataactat tttttaatca attataacca
     2161 atatttgttt tatttatctt cattttaatt cttcattcaa taatgtaata agattatttt
     2221 ttatttggat actattatat catctaaata tgttattata tatttggata ttttcatatt
     2281 aataggaggg attctacaat gataaattat atggtagaaa ttatatatag cgaaaaaata
     2341 agcgaaatag aaaacatgtt gaaaattgaa aatatacaat atgaatttac ttctcgtgat
     2401 ggctttatat tttttggttt ttatgatatg acagacgcat atagatttga gactttatgt
     2461 tcagatgaaa aaattcgatt taaaagaata taatagataa aaaagtacat atttctaaac
     2521 tatttgtttt tcaaatttag gttgtgtttt aatgtagtgt tgatattata aacaatttta
     2581 ataataaaaa agtagggaca ttatattaat atgctcctac tttttactca tacttattaa
     2641 tattatttat caaaattacc ctacaaactg gaatttatca ttctttcata attttaaact
     2701 atcatattta aaatatataa aattctacat gatttttgta taaacataat atcaaccatt
     2761 atccaattta aaaatatttt ttagtaattt ccatttcatt ttctcttaac atattaatta
     2821 aacttttata tgtaaatggt gtatttacat attatttaat atccaacaat tatataaaga
     2881 aatgtgctct ttgccttata aagaattata aagccaattc gttttattat attatttttc
     2941 tttatacctt aaaaacttta ccttcaaatt cttatttatc tatatattat aaaaatgttc
     3001 ttaacctctt ttacgagatt ttcttgacat attatcagtt agataaaata attaaataat
     3061 ataatcagca agtatgtttt ttactctttt aagtaaattt ccacctgcac cccaatcaat
     3121 ttttaaaatt ccaattgcac tgcctgttga agtagcttta ataactgttt ttcctgtggt
     3181 attttcataa actactatta atcctgcatc actattattt cttgcgtaat acttctcaaa
     3241 agttgtaatt agtataaatt tatcatctcc taaattataa gaatcactaa aaactaaatc
     3301 tgcatcaaga ttttctttta tcattttttg tgcctctaag ggtgtaattt ttacagtaaa
     3361 tatttctgtt gccatttttt atctcctcat aagaatttat tttagacaaa acataatctc
     3421 tagtattttc tgatatattt atcaaacaat atccctattc ctcctaacag agctaatata
     3481 gaaccaaaat ttctatattg agaaatagat tggtctagat aaatattata ttggtcagta
     3541 tccatgattc caccattttg ttgaataata tgctctgcac taaattcacc taaacctata
     3601 cagcagaata atactactac tccaattaca aaagcaaata tacatactgt taatttaaaa
     3661 tccttatttt ccatagttat ccctctcata agctaaattt atttgattat ttttactaga
     3721 ttttatatta ctcttattat acaatagaac atatatttac atctatttcc aaaattgtaa
     3781 cctttatagt tttatatatt taaatttttt aaacaaaaaa tacacatcta taaattataa
     3841 atgtgtattc tgcaccatta ctaataatat attatacttt tttacaaatt tctaattgtt
     3901 tataactatt caaaaataaa attcatataa aataattcta ttgtacaatt gaaactttaa
     3961 taatgtctcc attttttctc tctaagtgga ctacttctcc agttaaatca tagtcaaatg
     4021 ccttaactaa tatttcagta acttttttac catctactac atcatctttt ccattaaata
     4081 aatccaaagc attattctca atcattctct ttgcaaaatt tacaggtaac tttatatgta
     4141 ttttatcgcc cgtatgtgat aagactctta ttcttaaaaa cttttctaaa ttgtttcttg
     4201 gtatttgttt tgatgccatg ctattaaatc actctccttt attttttatt taataataaa
     4261 ttttattctt aaaaatatct ttatatgtat ttattatgaa agtaacacta ttattttcta
     4321 atcttgtttt atattccttc ttctttaata tatttattta ataaatatct atgatttata
     4381 ataattgtat attttgagat tttactttac aattattgta tctaaattta acaaaatatc
     4441 ttattagaat atttcaatta aaacaaaact ggtttattat ataaaaaata aattag
//
LOCUS       DAEMHW010000257         4495 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEMHW010000257 DAEMHW010000000
VERSION     DAEMHW010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.259"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..57
                     /locus_tag="KPK67_003516"
     CDS             <1..57
                     /locus_tag="KPK67_003516"
                     /inference="COORDINATES: protein motif:HMM:NF036802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460433.1"
                     /translation="LYQKTKRNPMILPIIMEV"
     gene            263..463
                     /locus_tag="KPK67_003517"
     CDS             263..463
                     /locus_tag="KPK67_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBG2460434.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            517..1680
                     /locus_tag="KPK67_003518"
     CDS             517..1680
                     /locus_tag="KPK67_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG2460435.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(1891..2313)
                     /locus_tag="KPK67_003519"
     CDS             complement(1891..2313)
                     /locus_tag="KPK67_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBG2460436.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(2334..3170)
                     /locus_tag="KPK67_003520"
     CDS             complement(2334..3170)
                     /locus_tag="KPK67_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2460437.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            3468..4064
                     /locus_tag="KPK67_003521"
     CDS             3468..4064
                     /locus_tag="KPK67_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBG2460438.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            4094..>4495
                     /locus_tag="KPK67_003522"
     CDS             4094..>4495
                     /locus_tag="KPK67_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBG2460439.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDDEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDI"
BASE COUNT         1672 a          545 c          693 g         1585 t
ORIGIN      
        1 ttatatcaaa aaactaaacg aaatccaatg atacttccta tcataatgga agtataatat
       61 aaaaaaacta taaatttgaa aattagacct cttgtaattt ttaaagtaat atcaaaaatc
      121 caattgatat tatcttaaga attacaagag gtctttttaa tactcatttt aaaaaaatat
      181 atattacata aaataccaat tataacaatc ttttattagt aaaaactact tatatataaa
      241 ttttactgat aggaggattc ttatggatga tgtttcaaga caaaatgcta taaaagcttt
      301 aaaacaaaca aaaatggaaa tagcaggaga gtatggaatg aattatgaag atgcttttga
      361 aataatagaa aatgcgtcta ataaaggtgt tttagaaggt tactttaaaa agcttgaaaa
      421 gaaaaaaaat ttaggtcaag gtatatctag gcatttagaa taaaaaaagt taaatgtgag
      481 aaaataagtt taaataaact ttaagaggtg acatcgatga aaaaattagc tagtactgct
      541 ttggctatat taattgcgct tactccttta agcttttctt ttgcaaataa taaggaaaat
      601 gctgatgcca accaactaaa tatatcttca aaatcagcaa ttttgatgga tgttggtagt
      661 ggacaaattt tatatgaaaa ggatgctcat aaaaagcttc ctcctgctag tgtaactaag
      721 gtaatgacta tgctacttat agtagaagct ttggattcag gcaagattaa gcttgatgat
      781 gaagttcaag taagtgaaac tgcatctagt atgggtggta gtcaaatatt cttagagcca
      841 ggagaaactc aaaaggttga tacattatta aaaggtatag ctgttgcttc tgcaaatgat
      901 gcttgtgttg ctatggctga acacttagct ggaagtgttg aaggatttgt tgatagaatg
      961 aatgctaaag caaaagagtt aaatatgaat gatactcatt ttgcaaatac aaatggactt
     1021 ccagttgcaa accactatac atctgctcat gatatagctt taatgtctag agaactatta
     1081 aaacacgaaa tgataagtaa atacttaact acatggatgg ataaggttgt agtaggaaag
     1141 aaacaagtta cagtaggtct tgcaaatact aataaactta taaagcatta tcaaggagca
     1201 actggtgtta aaactggttt tactcaagaa gcaaaatact gtttatcagc atctgcaaag
     1261 agaggtaata ctcatctagt tgctgcaact ttaggagcag agacttctcc agaaagattt
     1321 aatgatgcct ctagcctttt aaactatgga tttgctaatt atgaaagtgt taagttatgt
     1381 agtaaaggtg ataatatagc aacacttaca ttggataagg ctgatgaaaa caaagttaaa
     1441 ttggttgcta aagaagattt aaatgctctt attaaaaaag gaagttctaa agaatttgag
     1501 aagaaaattg aaatagtaaa aaatcctaag atgccaatta aaaagggaac tgttttagga
     1561 aaaataaaga tatgtaaaga taaaaaagta ataggcgaag tggaattgat aaatactaag
     1621 gatataaata aggcaagcta tctacaaatg cttcaaagaa taattgataa tatgatataa
     1681 ataattatca gatgaaatat aattataata cacaaagata tgattataat acacataaaa
     1741 aataccctta tgtaattatt tgccataggg gtatttttat ttaccatata gaagtatttt
     1801 ataatcttat gatttttata gaaatataga aaagtttatg tatcttaaaa tatcatttga
     1861 tttaatatca tatatagggt tgtttaacaa ttatctaagt aaaggcatac taccagtaag
     1921 tttattcact ttctttttat tcccataaat tgctatccca aaatactgta actcatcttc
     1981 tggaacacta gcaatctttt gtgtaaattc ttcataaaga ttgcaacttt gtgctacatc
     2041 tgaaaaatct acaacagtta aatcgttaaa ttccaaagta tataattttt tacgtaaatc
     2101 ttttattatt tctttgtttc cttttaaaat aggtacagga atgtcaataa ttcctaagtg
     2161 gctattacct gttttgtcaa ttacatctgg cccaacagtt tctggggcat gttttcctag
     2221 tgtaattccc attatagctg ctgtattgga tataattccc attggtaaat tttcgtctat
     2281 taccatgaca cattttgaat ttttagtttc catttttatt tctccttttt accttattta
     2341 aagttgtttg aatcatcatt tataaatata ttttgatact gctttggtgt aagaccaata
     2401 aaattcttaa aaaagtttgt aaagtgactt tggtctacaa atcctgtttg tatcgcagca
     2461 tctataggtt ctacgccttt ttctaaaagc tttttagctt tgtttacccg tatattttct
     2521 aggtatctat atggtgtaat accttttaat ttcgtaaaat tacgaagtag agagtactta
     2581 ttcatacctg ctatagtgct taattcgtca agtactatat gttcagcata attattttct
     2641 agataatcac atactgcttg aatttctata tttgtatttt ccaagttaga ttgcaaattt
     2701 ggctctgtat gttcttcaat taattgttca attaggaaga aaaaaagctc ttccttttta
     2761 aaatccaatt cttcttccat gattatatag tgtaattctt gtagtagagg tactagctca
     2821 ctgcgaaata taacaggttg attaaatttt ggtaaataat ttttacctgt aatttcaaag
     2881 acagtttttt tcattatttc tggcttaata ttaatacatc tgtaatcaag tactttatca
     2941 tctatttgtt cacaagtatg actatcaaaa gggttaaaaa gtagcaaatc tccagtactt
     3001 gtggtatact ctttattttt acaagataaa tatctttgac ctttttctat aaaaccaatt
     3061 acataatgtt catgaaaatg gtttgggaat ttttgcatta tgcctttaaa tctatatgct
     3121 tcaattttta attccctatc aaagcatact gtgcgttctt cttgtctcac ttaggtaacc
     3181 tcctttcatg ttatacaaag tgtaacacat atttttttat acttcttgta taatattgac
     3241 taagagacaa taatgtcaat tcatgtcaat ataatattac acttacagta ggtgtaatta
     3301 ttatattttc acaatgaata tgatataata ataaaagtta taaaataaaa agcaaatttt
     3361 aattttatgc ctagaagtta aaattattat aatgtaaaat aattttgatt ataagctatt
     3421 taattgttat aataatatta aaaatttaat ttaggagatg tacattaatg ttaagtatga
     3481 atataggaga aatagtagca agtttagttg gtatagctat ggcaatatca atacatgagt
     3541 ttggccatgc ttattcagct cacttattgg gagatgatac agctaaagct tatggtagaa
     3601 tgacattaaa tcctgtaaga catgttgacc caataggtct tttggctatg tttatcctta
     3661 agatagggtg ggcaaagcca gtaccagtaa atccaaataa ctttaaaaac tataagatag
     3721 gaaacctgat agtttcactt gcaggcgttt tttgtaatgt tttgactgct atactgtgtg
     3781 taattatcaa taagtatgtg catatgtatg ctataaatac aatagctgaa tatgtatttt
     3841 tatatagtat tggatttgca gcttttaact tacttccgat acctccactt gatggatggg
     3901 gagttatatc tacatttgta ccatataaat ggaatgaata tttgtataaa tttgaaagta
     3961 tgagctatat aattttatta attgctttat ttactggtat gtatagaata atattaacac
     4021 caatatatgc agtgttgcta aaaatagtat atttatttgt ttaatataat aaacagatta
     4081 aagagggatt gatatgaagt ataatataca attacaagtc tatgagggac cattagattt
     4141 actttatgat ttaataacaa agcataaaat agatataaag gatatatcta taatagatat
     4201 aactaagcag tatctaaatt atttgaaaat gctagataaa atggatttag agataactag
     4261 tgaatttata actatggcat caaaactttt agagataaag tctaaatatc tgctttataa
     4321 gcaaaaagat gatgaagaag acccaagaat agaacttatg gaaaagttag aagaatatag
     4381 aaagtttaag gtagcatcac aagatataaa agaaaatata acttatgtta atgagagatt
     4441 ttatagaaat aaagaagaga taattattga tgacaatgtg gatttagaag atata
//
LOCUS       DAEMHW010000258         4492 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEMHW010000258 DAEMHW010000000
VERSION     DAEMHW010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.260"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            481..1788
                     /locus_tag="KPK67_003523"
     CDS             481..1788
                     /locus_tag="KPK67_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460440.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQSKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1874..2136
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2262..3065
                     /locus_tag="KPK67_003524"
     CDS             2262..3065
                     /locus_tag="KPK67_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG2460441.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3103..3714
                     /locus_tag="KPK67_003525"
     CDS             3103..3714
                     /locus_tag="KPK67_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG2460442.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3746..4417
                     /locus_tag="KPK67_003526"
     CDS             3746..4417
                     /locus_tag="KPK67_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG2460443.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
BASE COUNT         1798 a          416 c          819 g         1459 t
ORIGIN      
        1 aaaagggaat atttacagtt taatggcagt tttaaaataa taaattaaag ctgtcatttt
       61 tatttaaaaa tttttagaaa aagtacaata gtgttaatgt gtgaattaga taaggtgtat
      121 ataataattt agatgatatg atataaaata tgttgtaaat taaatgtttt ctttgacaat
      181 atatacacaa ggatgataat atctatatga ataaaagata gtgtgtaatt tgataatatg
      241 taattataac attatgatat aattacaata aagtatgtaa taatctgtag aaatttcaag
      301 ctagttatct aagttttcgg ataactaaat ctaaacggaa tacataatgt attaaataaa
      361 tgatgatact aaaatgatac tatttttata gaaatttatt gaggtatata attaatgaaa
      421 ataatatatt aaattatata atatattgag ttttacataa aagcaaaggt gggaataaaa
      481 atgaaagaaa ttgctgatgc tataacatca gagtatttaa gctatggtat caatgaagat
      541 attagtatct atgaaggtag attttgcata tatttagata aaaaatatag atgtaatggg
      601 aaaatttatt ataaaatgac acctccaata tctataagtt ttaaagcaga cataggttgt
      661 gttgaagaga tagacaatga agatgacaat ttagctttag attatgataa tgctattctt
      721 gaagttcatg gatataaaat tatttctata acaataaata ctttaagtga atttagtgta
      781 gaaggttata tcaatgatga ctgcataaaa tctaagaact cctatgttga atatgtagat
      841 tttaatataa taaacttaga taaaatacct ggaaaactca ttaaatacaa tgataaggta
      901 tacgcaggta gaattgaatt tgatataaat gattatgttg taactataga caagcgatat
      961 gattatagaa aagaattaaa atcagaatta aagtctaaat ctggagcaat aataactcat
     1021 ataggtagga ttagaagaaa agatggtaga atttttagaa caaataatac aataaatctt
     1081 ttagatagaa tatcaacagc tttgagtttt atgtgtggaa gatatgtagg gttttgtttg
     1141 gccaaagggt atagaagtgg taatgaagtt tatagaatat ggaatgaaaa tcaaatatct
     1201 ccttttagat atgtaccaac atggtcagat acactttcaa attatcataa tatggaaaaa
     1261 tatataagcc ttatgtgtaa aaagctagaa gatttttatt atggttctgc tataaagagt
     1321 gttgttgatt ggtacataga atctcttgga agtgctacca tggaaaacaa tataatatct
     1381 gtacaaattg cacttgaaac tttgtcatat gtaattctag tggaacaaag taagatactt
     1441 acagatgaag catttgactg taatttggca tctaaaaata taagactctt acttgataca
     1501 tgtaagatac catatggtaa acatgaatta aatatatttg ataatataat aaaaaataaa
     1561 tttgatgatg gagtggattt agttatttat ttaagaaata gcatagttca tccaagtaga
     1621 aagactcata gagctgtgtt agaggttgaa gatatttgga atataatttc aataggaacg
     1681 agatatattg aacttgtttt actctttatt ttaggttata gaggagaata ttcaaacaga
     1741 ttagtagaaa gatgttatgg tgaagtggaa gttgtacctt ggaactagat ttttatattt
     1801 attttaaaat atatattgac atatattgcc ttatgctata aaatcaatat cataataata
     1861 attcagaata acattaaatg ctaagaaaag aaatagtaaa agtagttaat cttaaacaga
     1921 gagtttccga ttggtgagag gagcaataga ttttactttg aacacatctt tgagcaggac
     1981 tctgaaagag tagaaatact ttattaggag atttccggaa tccttgcacc cgttatagtg
     2041 ctagagtata ataattttat tgtacttaaa gaggttagtg tcgtgaggca ttaatgaaca
     2101 gaggtggaac cacgagagtt aactcccgtc ctctagcaga agctagaggc gggagttttt
     2161 taatataatt ttttaagcgc gcagagaatt atattgcaat agatacactt tttaaaaaat
     2221 aatcaaaata ttttaaaaat taaaagacga gggagaatat tatgaaaaat atattaaaaa
     2281 aagtaggaat atttacaatt atgttaggtt tattaggtgg agttgtaggt tgttcaaaac
     2341 cagataatga aaaagataag gatgcttcaa aggaatctaa aaaagaagta gttgtagggt
     2401 ttgataatac gtttgtacca atgggatttt tagatgaaaa gggagataca gtaggatttg
     2461 acgtagactt agctaaagaa acatttaaaa gacttggaat ggaagttaag tttcagccta
     2521 tagattggtc aatgaaggaa actgaattaa atgattcaaa aactgtagat gtattatgga
     2581 atggatattc tataacagac gaaagaaaga aaatagtatc atatacagaa ccatatttac
     2641 aaaataagca gataatagtt actctaagtg actcaaaaat aaattcaaaa gctgatttaa
     2701 aagataaaga agttggaaca caacaaggtt caacagcact tgatgcagtg gaaaaggata
     2761 aagattttat gaatagctta aaaggtggag caccagttct ttatgatact tatgataaag
     2821 cacttagaga tttagagata ggtagaacta gtgcagttgt tggtgatgaa gttttaatta
     2881 gatactatat gggacaaaag ggtgaagaca aatacaaggt attaaaagac gattttggat
     2941 tagaagatta tgttgtagca acttctaaag aaaatccaga actttgtgag aaaataaatg
     3001 aaacattaaa agaaatgaaa aaagatggaa cttttgacaa gatttatgat aagtggttta
     3061 aatagaagat aagttagctg ttaagaaggg ggcactaaag gcatgttaca aggtttagag
     3121 atagttatag caatgttttg tataacattg atagtatcaa ttccattggg gattggagtt
     3181 gcatttttaa gattatcaaa gaataaatta gtaagtggta ttacacaatg ttatatattg
     3241 ataatgagag gtacaccttt acttttgcaa atgatagtca tattttacgg attacctctt
     3301 ttaggaattg tatttgacag atttacagct ggagtagtag ctttcttctt aaattatgca
     3361 gcatattttg cagaaatatt tagaggtgga atacagtcaa tcgatagagg acaatatgaa
     3421 gcttcaaaag tactaggatt tgataagttt actatgtata agagagtaat ctttccacaa
     3481 gtttttaaaa gaatactagc acctatttca aatgaagtaa tcacattagt aaaagataca
     3541 tctttagtat atatattagg attaaatgat attttaagaa tatctcaaat agcaatgaac
     3601 agggaagcaa gtctgttacc attatttgag gcaggagcga tatatcttat atttgtagct
     3661 atacttacta agggatttga attacttgaa aaaaaatatt cttattatag ataaagttac
     3721 aatattatag gaggaggttt tttatatgtt gaaaatcaaa aacttaaata aatcatttaa
     3781 aaaaaataga gtacttaaag atatttcttt tgagttagaa gaaggtcaga ttggagtttt
     3841 acttggtaag tctggtgcag gaaagaccac aatattaagg tgtataaatg gtcttgaaga
     3901 atttgatagt ggagagatta taatagataa tgaggtaata aaaaataaga gagatatggc
     3961 aaaaatacga ggaaaaatag gtatggtttt tcaaaatttc aacttatttc ctcatatgac
     4021 agtccttgaa aatataattg aatccccagt taatgttttt aaagttccta gaaaagaggc
     4081 tgaagagagg gctagagaac ttttaagatt agttgattta gaagataaat taaattctta
     4141 tccatttgaa ttgtcaggag gacagcaaca gagagttgct atagctaggt catgtgcatt
     4201 gatgccaaaa gtcctttgtt ttgatgaacc tacttctgca cttgatattg atactattca
     4261 aagagtagtc aatataatga acagacttaa agacaaaggt atgacaatat taataattac
     4321 acatgatgtt gtcttctcaa ataatgttgc agataaaatt ataagtataa aagatggtat
     4381 agtagaaaat gttcaaataa aagaaaaaat tgtttagagt tttataaata taaactaggg
     4441 tagaatttta taatataaat aaagggcaag taaaatcaaa tatactatgc cc
//
LOCUS       DAEMHW010000259         4371 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEMHW010000259 DAEMHW010000000
VERSION     DAEMHW010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.261"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            182..1072
                     /locus_tag="KPK67_003527"
     CDS             182..1072
                     /locus_tag="KPK67_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG2460444.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            1290..2576
                     /locus_tag="KPK67_003528"
     CDS             1290..2576
                     /locus_tag="KPK67_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG2460445.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDNMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(2665..3108)
                     /locus_tag="KPK67_003529"
     CDS             complement(2665..3108)
                     /locus_tag="KPK67_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBG2460446.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(3132..3926)
                     /locus_tag="KPK67_003530"
     CDS             complement(3132..3926)
                     /locus_tag="KPK67_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBG2460447.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
BASE COUNT         1699 a          492 c          672 g         1508 t
ORIGIN      
        1 acatataact ttttagctat aattattatt gttattaatt gaaaagattt attagttact
       61 tgacatagtt tacactttta attatagtat aaattggaat atttagataa ttattattta
      121 atgtataagc taagttttta attaatatgg ttttatacat ttatatagaa aagaggtacg
      181 tatgagtagc acaatcaaat caaatcgaat aaaaggtata atgtttataa tagcatcagc
      241 gcttggcttt gcaatgatgt ctgcattcgt aaagctagct ggagatttac caagttttca
      301 aaaagtgttc tttagaaatc tggtatcagc tataatagct ttttggctta tcataaaaca
      361 taaaggaagt ctaactggta aaaaagaaaa tttaaaagta cttttatata ggtcaatatt
      421 tggaactcta ggagttatat ttaactacta tgcaattgac aagttgatgc tttcagatgc
      481 taatatgcta aataaaatga gtcccttcct tgtagtaata ttttgtgcaa ttgctctaaa
      541 agaaaaaata aatgtaaagc agataggagc tattataatt gcatttatag gagctttatt
      601 tataataaaa ccaacattta gtgtagaagt aattccatat cttgggggaa ttgcaggagc
      661 tgtatttgca gcaatggcat atacttgttt aagagttctt ggagatagag aagattacta
      721 tacaattgta tttttctttt cagttttttc attagttact gtagggccaa ttgcttttgc
      781 agtatatgaa ccaatgaccc ttatgcaact tatatatcta ttactaggtg gagtatttgc
      841 aagtctaggt caatttggaa taactctagc atataaatat gcacctgcta aggaaatctc
      901 tatatttgac tatagtaata taatcttttc agcaatttta agtatattct tattcaatgt
      961 ctatccagat atgttaagtg ttgttggata tttaatagta ttttcggcag cattttatat
     1021 gtttttatac aataagaagt tagataaatt agacaaagaa aagaagaagt aaactagata
     1081 aaagtgtttt tataatttgt attgaatatt taagtctttg tattaaaatt aaagaaaatt
     1141 taattaaaaa tgtttggtat attaacttat aacagcagtt aaaattgaaa aattataact
     1201 gctgtttctt ttattaaaaa tatccaaatg tctgtaaaaa tagactataa tagagtagta
     1261 taaataatat aaaggggata aattaaagta tggaaaaagt gaaagttgct gtgattggag
     1321 caggtaatag aggaacgtat gcatatgctc catatattta tgaaaattct gatgtatgtg
     1381 aaatagttgc tgtagcagaa cctaaaaaag gaagacgaga actatttaca caaaaatata
     1441 acctagatag taaaaatgta tttgaaactt tagaagactt ttttaaacat gacaatatgg
     1501 cagatgcagt aataatagct acaaatgatg atagacatta tgatgtagca aaactagctc
     1561 ttgaaaaagg ttatcatgta ctattagaaa agccaatgtc taatagttta gatggtttgg
     1621 tacatataga tgacttgtgt gataagtata aagataagat ttttatgatt tgccatgtac
     1681 tgagatattc tccattttat aataaattaa aagaaattgt agaaagcaaa aaattaggag
     1741 agctagttag tatacaatat aatgaaaata taggatattg gcattttgca catagcttta
     1801 ctagagggaa ttggagaaat agcaatgaaa ccagcccttt aattttagca aagagttgtc
     1861 atgacatgga tattttgtta tatttagtag gaagtagatg caaaaaaata tctgcatttg
     1921 gaagcttaaa gcatcttaat aaccaaaatg ctagtggaga gatggcacaa aattgtcttc
     1981 agtgtttagt tgaaaaaaaa tgtccatatt cagcaaagag aatttattta gaaaaagata
     2041 gaagtataaa tagagctgtt cacataaatc caactgaaga gaatctatta aatatactta
     2101 agacaagtcc atatggaaga tgtgtttaca gatgtgataa taatgttgta gacaatatgg
     2161 taaatatact acagtttgaa aatggagtga cggctacttt taatttatgt gcatttacta
     2221 aagaaaatgg aagaactata aagttaatgt ttagccatgg tgaagttggt ggggatttaa
     2281 ataaaaatga gataagaatt aaagaatttg gaaaaaacga agaaatagtt atgaatccat
     2341 caaatcaaca gaatatggta gaggaatatg atagaaatct tatagctgaa tttattaaat
     2401 tagtttcaaa taaagaacta gaaaagggta gagtagcagc aaaagaagct atacaaagtc
     2461 atgtaatggc atttgcagca gaatattcta gggtcagtga tgaagtagtg tatatagaag
     2521 aattttttga tagtgctaag caaatgacta aggagataga agaaactata ttttaattaa
     2581 taaaaattta ttaaataaaa taaagataga ttaaataata gaaatacgaa aatatgtgtt
     2641 ctattaagga atctatcttt tttattaagg ttttttctta tccctatcaa aaaaatccat
     2701 acattctgat aaaagataag aaagtaaagc gaataagatt tctgtactta aaggaatcgt
     2761 tactgaatcc ataaaggtat caattatgtt catagctaga aaagttagta taaaatcaat
     2821 tataaattcg caaagtttat atattgagta tggtaatttg tttacatatc tgaatatatc
     2881 taaaatagta gtgcatagaa aatcaattgg agtggttata caaaaatata ttaaaaagaa
     2941 aattaataca gctccaagag atttatattc aaatcctaag aatgataaga agccaattcc
     3001 tataaccgtg aaaatacaaa aagttatacc aaaaaaaaca gcaaaaaata aaaatacaga
     3061 aataaattcg cctaaaggaa tccttctttt attacttatc ttaatcaact ccttaattta
     3121 tgatatgaaa tttatataaa taataacatt cttaaaaatt cataatagtc ctctgtttca
     3181 aatagtactg tttgagaatc tatttttttc atatttggat aaaaagaggc cttaaaagct
     3241 ttataatgtt ctctaaattt tatttctatc ttaaatttat ctggcaattt tattaagtgc
     3301 ctagataaat ctccagataa agcttttttg acaccttttt caattttttt tagtgcaagg
     3361 tttggatgga ttgaaattga accatttcca attccttttg acacaggaac agtgactata
     3421 tttggattta gtttttttgc actctcacaa agcatttcat ctccacttaa aaatgctaca
     3481 ggaacattat aatatgcagc aatataagca tttatagtaa attcagatgc tatttcatca
     3541 tttattttaa aatagtcata atcaatatcc atagtatggg aaagtggatt accattacaa
     3601 gtagctccag agtgatagcc agtgaatata catgcatcaa agctttcatc aattccagcc
     3661 atcataacta gtgggtcttt ggtccatcct ctaagtattt tgacgttttc aggtaaaata
     3721 tttggattaa tatttctacc actgtcatgt gcatctttaa caagtatttc atgtacacca
     3781 gattgagtag ctccatcaca tgctgctgtt atctctttac tcatttgtgt tctatagtgt
     3841 tgtgaaaatt cacattcaag ttttgtttca tcccaattta caataccaca agtaccttct
     3901 atatcagcac ttaaatacaa tttcataata tataccccct tatgtttatt attgatgata
     3961 ataacagata acagaaaata cataatcaat aaagggcata aaaatattat gtcttactat
     4021 taactattat taaatctgta actgaaaaat aactaaattg gtataaaaaa atataacact
     4081 atgtgtcatt tatttatata gttttttata tctgatgttt atataaaatt gtaacatata
     4141 agtggttaca tttactagtt taacatagtt aaaatagtta ggtattattt ttaagttaaa
     4201 ttgattatta taaaatcata gaataaaaga aaaatttaat ttattaaaat gcatgaaatg
     4261 ttttttcctc aacataatgc taaattaaac tcaaaaagaa ataatagaaa aatcttatgt
     4321 agcttagaaa gaatgtgaat gatttaaggg aaaaatagaa atttattata t
//
LOCUS       DAEMHW010000260         4237 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEMHW010000260 DAEMHW010000000
VERSION     DAEMHW010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.262"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    327..565
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            629..2548
                     /gene="thrS"
                     /locus_tag="KPK67_003531"
     CDS             629..2548
                     /gene="thrS"
                     /locus_tag="KPK67_003531"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBG2460448.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            complement(2899..4092)
                     /locus_tag="KPK67_003532"
     CDS             complement(2899..4092)
                     /locus_tag="KPK67_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2460449.1"
                     /translation="MSEKRRDNKNRILRTGESQRKDGRYAYKYIDTFGKPQFVYSWKL
                     VPTDKTPAGKRDDIALREKEKEIQKDLDDGIDHIGKKMTVCQLYAKQIRHRANVRHGT
                     KQGRKQLMRILQEDKLGACRIENVKLSDAKEWALRMKEKGYGFKTINNHKRSLKAAFY
                     TAIQDDCIRKNPFDFQLNTVLEDDTEPKEPLSPTQEAAFLSFVQHDKVYQKYYDEIII
                     LLGTGLRISELCGLTEADIDLDKQLINVDHQLLKIADVGYYVETPKTKSGNRVIPMSE
                     KVLEAFQRVLNKRKYAQPVILEGYTKFLFLNRNGLPKVAVNYESMFRGLVRKYNKTQK
                     VALPKVMTPHTLRHTFCTTLANAGMNPKALQYIMGHSNINMTLNYYAHTTSETSITEM
                     KRLIA"
BASE COUNT         1392 a          641 c          831 g         1373 t
ORIGIN      
        1 ttactgtact acttattaac catattaagt attgattgta tttaagtata caaaacctaa
       61 aaagagtaca aaaattagaa gttagtttta ataaaaatta gttttagtat ttatctagtc
      121 atatctatca tgcatgtgga tacgttttaa aaattttaaa tttatgaaaa tattataata
      181 tcactataaa tcaagagatt aatttaatat aaaaatataa gaatattggt ttacagctga
      241 ttgagatgaa aaataatggg taattaatat attacattaa tgttgattga tgtataacta
      301 tctgttataa tgaaaatata ttaaaaatca tagtgttgaa gaggaagagt agagtagaaa
      361 ccattctcag agaatttggg tctatggctg aaagcccatg taataggtct atttgaaaac
      421 taccttggag ctgagcttat tagcagtatt tgtatttgta ctatacgtga aagtgctcgg
      481 gtgacgacct tatagtcatc aagagggttg tatatacttg tatacgccaa cttgggtgga
      541 accgcgaatt aatatcgtcc caaacatatt tggggcgatt ttttattaca aaaaatctaa
      601 aaattatttc aataaggagg aaaaaacaat gattaaagtt gctttaaaag atggctcaat
      661 aaaagaattt gaaaatgcta tttctgttat ggatgtagct aaatccatta gtgaaggatt
      721 agctagaaat gtggtagcag cgtctgtgaa cggagaggtt gtaggtctgg accacattat
      781 agatactgat tgtgatttga atttatttaa gtttgaagat aaagaaggta aggaagtttt
      841 tagacataca tcagcacata tattagctca agctataaaa agactttacc cagaagcaaa
      901 gcttgctata ggaccaagta tagaaaatgg attctactat gatatagatt tagaccatag
      961 attagttcca gaggacctag agaaaataga agctgaaatg aaaaaaatag ctaaagaaga
     1021 tttaaaaata gaaagatttg aacttcctag aaatgaagct ttagagctta tgaaagaaca
     1081 aggagaagac tataaggtag aacttatatc tgatttacct gaaagtgaaa taatatcttt
     1141 ctacaagcaa ggtgatttta ctgatttatg tagaggacca catttaccat ctactaaaaa
     1201 ggtaaaagct gttaaattgc aaagtgtagc aggtgcatat tggcgtggtg acgaaaataa
     1261 taagatgctt caaagaatat atggaacttc ttttgagaaa aacaaagact tagaagagta
     1321 cttacattta ctagaagaag ctaaaaagag agaccacaga aaattaggta aagaattagg
     1381 attatttatg atacctgaag aaggtccagg attccctatg tttttaccaa aaggtatgga
     1441 acttaaaaat gaattattaa aattctggag agaaatacac agaaaagctg gatatattga
     1501 gatagaatct ccaatcatat taaatagaaa attatgggag acttcaggac actggtatca
     1561 ttacaaagaa aatatgtata cagttaaaat agatgatgag gattatgcta taaaacctat
     1621 gaactgtcct ggtggattaa tttactataa ctctcaacta cattcatata gagatttccc
     1681 aatgagagtt gcagagcttg gtagagttca tagacatgag ttatctggtg cattacaagg
     1741 attaatgaga gttagagcat ttactcaaga cgattcgcat atattcatgt taccagagca
     1801 aattaaggac gaaataaaag gagtagctaa cttaatagat ggtatctaca aaacttttgg
     1861 atttgaatat aatttagaat tatctacaag accagagaac tctatgggaa gcgacgaaga
     1921 gtgggaagca gcagaaaatg gattaagaga agctttagaa gagttaggtc taccatatac
     1981 tattaatgag ggtgatggag cattctatgg tcctaagata gacttccatc taaaagattg
     2041 tttaggaaga acttggcaat gtggtactat ccaattagat atgcaacttc caagacaatt
     2101 tgataatact tatattggtc aagatggtga aaagcataga ccagttatga ttcatagggt
     2161 tgcttttggt agtatagaaa gatttatagg tatattaata gagcattatg ctggtaaatt
     2221 cccagtttgg ttatctccaa ctcaagttaa gatacttcct atatctgata aatttatgga
     2281 ttatgcaaat gaagttaaga aagagttatt tgataagggt ataagagttg aattagatga
     2341 tagagctgaa aaaattggat ttaagataag agaagctcag cttgaaaaag tgccatacat
     2401 gttaatagta ggtgaaaaag aagtagctga taataatgtt tctgttagat caagagataa
     2461 aggtgaaata ggtagtatta aattagatga atttatagct agtatttcaa aagaaattga
     2521 atcaagagaa agtattattc aagactaata tatattatat tagtagctaa tatattgtta
     2581 tataagataa gtttaatcaa tatgttgata tgttgatgca tagtgattaa atttatgaaa
     2641 agatgagtat aattagaggt acagttaatc atatttttat gattaatgta cctctagttt
     2701 tttgtatggt agatttatta ataaatcaca aagtaatttt tgattatctt ttcaaaagtt
     2761 cttatatgcc ggtatagcct attgttacag gcttttccgg catttttcat tgtggaagat
     2821 actctcaaat cttcttatat ccccgtatgt ttttgtgttc aaaagtagta tgtggagtag
     2881 taaattctcc atttactact atgcaatcag ccgcttcatt tcggttattg acgtttcgga
     2941 agttgtgtgt gcgtaatagt tcagcgtcat attgatgttg gaatgtccca tgatatattg
     3001 caatgcttta gggttcattc ccgcatttgc taacgtggtg cagaatgtgt ggcgtaaggt
     3061 gtgcggtgtc attacttttg gcaatgcgac cttttgcgtt ttgttgtatt tccttacaag
     3121 cccccggaac atactttcat agttgactgc cacttttggc aagccgttcc ggttaaggaa
     3181 cagaaacttt gtgtagccct ccaaaataac aggctgtgca tatttccgtt tgttcagcac
     3241 ccgttgaaat gcttccaaca ctttttcact cataggaata acccggttgc cgcttttcgt
     3301 cttaggcgtt tccacatagt agcccacatc tgcaatcttc aaaagttggt ggtctacatt
     3361 gataagctgt ttgtctaagt caatgtcagc ttccgtcagt ccgcagagtt ccgaaatgcg
     3421 aagccctgtc cctaacagta tgataatctc gtcgtagtat ttctgataga ctttatcatg
     3481 ctgcacaaag gacagaaaag ccgcttcctg cgtaggggat agaggttcct ttggttccgt
     3541 atcatcttca aggactgtgt tcaactggaa gtcaaatgga tttttacgaa tacaatcgtc
     3601 ctgtatggct gtgtaaaagg cagcctttaa agaacgcttg tggttgttga tagtcttgaa
     3661 gccgtaacct ttttctttca tgcgtaacgc ccattccttt gcgtcggaga gcttcacatt
     3721 ttcaatccgg caggctccaa gtttatcctc ctgcaaaatc cgcatgagct gttttcgccc
     3781 ctgctttgta ccatgccgca catttgcccg gtggcgtatc tgctttgcgt agagttgaca
     3841 gaccgtcatt ttctttccga tatggtcgat accgtcgtca aggtcttttt ggatttcttt
     3901 ctctttttcc ctcaacgcta tatcgtcgcg ctttcctgcc ggggttttgt ccgtaggtac
     3961 taacttccaa gaataaacaa actgcggttt cccaaaggta tctatatatt tgtaggcata
     4021 tctcccgtct tttctctggc tctctccggt gcggagaata cgatttttat tatcccgtct
     4081 tttttccgac atcgttatgc tcctttccat aatggaaaga gccttgatat gcttgactat
     4141 attgtaccac attcaaggct cgatttcact atcagattgc gtccaccgta tcaatcattt
     4201 tttcaaactg cttacgctta atctgaatac ggtttcc
//
LOCUS       DAEMHW010000261         4189 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEMHW010000261 DAEMHW010000000
VERSION     DAEMHW010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.263"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(23..1468)
                     /locus_tag="KPK67_003533"
     CDS             complement(23..1468)
                     /locus_tag="KPK67_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG2460450.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEEARYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRIQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     YDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
     gene            complement(1490..1843)
                     /locus_tag="KPK67_003534"
     CDS             complement(1490..1843)
                     /locus_tag="KPK67_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG2460451.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(1872..3119)
                     /locus_tag="KPK67_003535"
     CDS             complement(1872..3119)
                     /locus_tag="KPK67_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG2460452.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTIVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            complement(3127..3444)
                     /locus_tag="KPK67_003536"
     CDS             complement(3127..3444)
                     /locus_tag="KPK67_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG2460453.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            3686..4189
                     /locus_tag="KPK67_003537"
                     /pseudo
     CDS             3686..4189
                     /locus_tag="KPK67_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         1399 a          769 c          452 g         1569 t
ORIGIN      
        1 gttgtttata tttaaaaact atttactttt ctcttaattc ttctccatta gttcttatta
       61 cattttgata ccaataaaaa ctatcttttt tataacgttc tttacttcca tttccattgt
      121 tatctatatc cacatatata aatccatatc ttttttccat ttcttgagat gaacaactta
      181 ctatatctat tggtgaccac atacaataag ctaatatttc ggttccatca gctatagctt
      241 cacctatttg ttctatatgt tttcctaaat aatctattct gtaactatca taaacttttc
      301 catcttcaaa tttatcatgc attccaaatc cattttcagc tatcattatt ggcttttggt
      361 atctatcata atattgagat aaagtattgt ataatccttt agggtcaatt gcccaccccc
      421 aaggatttgc ttttaaatat ggatttttta tattgtcagt tggattcatg gtgtttttac
      481 ttgctgatac tgttgtagaa taatagtatg ataaagctag aaaatccata gtattttctt
      541 ttaagatttt atcatctcct tcctccataa ctatatttat gttattttct ttgaaaaatc
      601 tcttaatata gactggatac tctcccctaa aacttacatc agtgaaaaaa tattgtattc
      661 tatttctttt taaagcaaac accacatttt cagggtcgca atcttctgga taagatgtac
      721 aatctgccat catcatacct atcatcatat ctgaatttat ttcttttcca agcttcttag
      781 tcaaagctgt agctatgaat tgatgatgaa tagcttgata tctagcctct tctacattat
      841 ctacttggtc tacacaaaat gcaactgaat taaatgattc ataatgtatt aaatttattt
      901 gatttataac tatccaatat ttaactttat ttttatatct atttagtatt aattctgcat
      961 atctaacaaa gaaatctacc acttttctat tagaccatcc tttatatttt aatactaaat
     1021 tcaaaggagt ctcatagtga agtagtgtta caataggttc cataccatta tctattactt
     1081 catttataag attatcataa aactttaatc cctcttcatt aggctctaaa tcatctccat
     1141 ttggaaatat tctagcccaa tttattgaaa atctaaaaca ctttaaccct agctctttca
     1201 aaagctttaa atcttctttg tatgtatgat aaaagtctat tccactacgc ttaggataat
     1261 atccattatt atctttcata gcaaacttta ttctttctat agtcatatca gactcataag
     1321 gtttagatat atctctatct ttatcataca tatgtacatc agctaaacac caacctttgc
     1381 caccttcatt ccatgcacct tctaattgat tagctgctac agctcctccc cataaaaaac
     1441 ctttaggaaa cttacccaat ttaatcactc cttattttat ttatttaaat catttactat
     1501 tatttacctt atattctata gactttacta ttcctactaa agacatagtt atatttaact
     1561 cgctgaatat ggtcataaga gtatcttgag catgtgcaaa taacagcgaa tgtgctcttt
     1621 tctctccacc agcttcccct tgtataatct tggtttgagc tctatgtgct tctgtcataa
     1681 acccttttgc tttagctagt tttaaatcag catttctata atcaaacttt tccaaatcat
     1741 taagagcatg tttaatttct gttctagcct cacctgcacg aagtattatt tgcattgcca
     1801 cctctactaa ttcttcatct attatatgtt cttcaattgt catatttata atttcctttc
     1861 aatttctaaa tttaaacctc ttggctctct aattttatct cttctttctc ataagcttta
     1921 aaaaatggat accatatcat aaatagcaat actatattta ttgctaaaac aataagacct
     1981 ctaacatctt gatttaccat aaaagtagat ataccaaaag gaacatacca taaattaaac
     2041 accttacttg gaattgtaac aaatcccata ctcattgata aataagtagt tattgatatt
     2101 actattgtac ctatccacat aggtaacatt aaaaatgggt tccatgccac tggagctcca
     2161 aatattactg gttcatttat attcattata gaagggacaa tacatgctct tcctattgct
     2221 tttattcttc caactctgga taaacacatc attataacta atggtaatgt agcacccaac
     2281 cctcctaaag ttatccaacc tgtttgaata acttcgtaaa catatatatt agttgctact
     2341 cctcctgatg ctactgctgc tgcattagct gcaattgcac caagttgaat tgaataagtt
     2401 ataggagata gtacccaccc acttattcct aaagaatata taaatactac taagaagttt
     2461 aagagtacaa atccaggtaa tgtctgtgct atatctgata ctggtttaaa tactctaact
     2521 attatttcaa acatgtcaaa gttgagtcca taaattaatc cccagcctac aaacattatc
     2581 actgttatag gtaacatatt atcaaaccac ttgcttacaa aatccggaag tgctgaatct
     2641 tccccgaaaa atgaaaaaga accaaataaa gacattatta atccagagaa taatcctgca
     2701 actagcgata caagcattcc ttctcctcca aacctgttga agttaaatat agctccttca
     2761 ttcgtaatct ctggacgaat catcattaac attaatccag cacctgcaag accagctact
     2821 acactcatac tttcaaattt cttattcttc attatttcca taggcaataa aaatgctata
     2881 aataatccta aaagtccaaa tgtatattga tttattggac ttaaatcagg taaaaagctt
     2941 atgtaattct tcaagacaga aactatagtt actaatgagc ctacaaatac cattggcaaa
     3001 actttcatta tagttgattg aactgattgt acccatatat tcttagtcat attatttaca
     3061 acaggagtaa atttttcact taaaaaatct ataaatttat tcataaacac tctccccata
     3121 aatattttat ttgtttgata ttagttctag tgcttcttcc aaagcctttg caccatctag
     3181 tcttccatat gcagttttag atatacatgc tactggaacg ttatattcat tagccttttg
     3241 ttgaatttct tccttcatat aatctaaatg tggtcctagt aataaacaat ctatttctga
     3301 taaatactta tctatctctg actcacttct cgcttttata tctgcttcta tacccttttt
     3361 ctttgcactc tttctcatac tagttgccat aaaacctgta cttgctcctg aaccacacgc
     3421 caaaagcact cttatatttt tcataatgat acctccaata tttttatcca ttaatttatt
     3481 tcttaatgaa ctttttattt tattatgttt atattgtact taccaaataa gttcatttca
     3541 cataaatata tttcctaata ataggaaatc agttactctt aattaaacat attaatatag
     3601 tataatgtat cttataatac gtacattcaa ttatataatt atcatatcat ccagatttta
     3661 ttttaaaact atgcgaggga atctaatgaa aacatctaaa aaagaactat ttttaaattt
     3721 cataaaaaca caacatgatt ggatagattc atctacattg gctaattatc ttaatgtctc
     3781 aacgcgttca attagaaaat atgtaaacga aattaattcc aatggtgaat ttatattatc
     3841 ctctaaaaaa ggatataaag taaatctcaa taataattgt caaaccaagg ttgatagttc
     3901 agaaaatata tcacctgata aaggttaaat ttaattttaa aggaacttat agttaatagt
     3961 aatggaatta atatatttga tttaagtgaa gaattgtttg ttagcccagc tactattgaa
     4021 ggggatatag tcaaagctaa taaatttata ggaagttata atttaaagat aaagcaatcg
     4081 aaatttttat tgaaactaat tggaaatgaa tctgcaaagc gcaagttaat gagctctata
     4141 atatttaaag aaacgggaag tgatttttta agcttatttg atgtacaaa
//
LOCUS       DAEMHW010000262         4141 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEMHW010000262 DAEMHW010000000
VERSION     DAEMHW010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.264"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(307..1176)
                     /locus_tag="KPK67_003538"
     CDS             complement(307..1176)
                     /locus_tag="KPK67_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG2460454.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITQEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDSLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
     gene            complement(1480..1950)
                     /locus_tag="KPK67_003539"
     CDS             complement(1480..1950)
                     /locus_tag="KPK67_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG2460455.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
     gene            complement(2748..3137)
                     /locus_tag="KPK67_003540"
     CDS             complement(2748..3137)
                     /locus_tag="KPK67_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2460456.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT         1380 a          696 c          273 g         1792 t
ORIGIN      
        1 attatcataa atataataat tctatttttt taaaataaat atttataata atctaaaata
       61 aatatttata aattctaatc aatcctaaat aataatctaa aatacatcaa aaaataaact
      121 aaaaaatatc gaaaaataat ttaaaagaca tcaaaactaa atcttctaat cagcaattaa
      181 aagtctattt tcttagttta aaaaataaag atagattttt tcaaaacact ggataaatag
      241 gtaaaaaaat aaacagttag taaaataaat ttttactaac tgtttatttt tattgtttaa
      301 tatgtcctat aaatcctcta agtaatattt tccttgcaaa aattctgatt ctaaacctag
      361 atttttaaaa taatcaacca tgcccataca cgcaaatttt tctgtagaat aatgagtagc
      421 acctataaca ttaattaaat tatcttttgc tatttgatgg aactctaata ctggttcaaa
      481 atgttttaaa ggcttagtaa acccagtaat atatagattt ataccaagtt cagcaacttc
      541 tcttgctaca aatggataac ttcctccccc tgctgcaata gcaactaaac tatcttttaa
      601 tacagaatct ccataatcat acaatttcac atcatgacca acagattttt taattaattc
      661 cttaatctct tctattgatt ttagtgatgt tttacatata actcctacct caattttatc
      721 gtcatattta caaaatggtt tgactatttc taaacccata gcttttgcaa aactaacaga
      781 agttgaatag tcactatagt tgtctagtgg agaatgtaat acataaaaag aaattctacg
      841 atttttcatc tcttccatat aagatagtgg tatgtcgtga aatggaaaac cttcatctga
      901 tgctatgtat cccattgcat gatgtgaaaa aattaaaata tctttttcat ctttatctat
      961 aacctgctta attatttctt tatcaggaaa agtagttgtg tatactttat taatattttg
     1021 agcattatct aatactaatc ctatgtactt ttttctaaat tcttgagtga taaaatcatt
     1081 ttcaaaattc ataaaactcc aatcatcatt aatattttta atattaaatt ctaaatctaa
     1141 ttttttataa agttcaattg ccttaataat aaccactcct ttataataat attgaattac
     1201 tacatattaa tatagaaatt tccgataata aactttatac gtttaagtat tcctacttca
     1261 aattaaaaat attataaata ttcaaattta ttaatatatt ttaatattat atttattttt
     1321 tctttattaa tattcccctt atttttattt attttttctt tattaatatt ttctttattt
     1381 taatttaatt tatgacattt gtcaaatatt agtcctatat attatcttga attagttcta
     1441 ctacaattaa tttctactaa ataattataa tttaaacaat cattccttac tattttcaca
     1501 ctgctttaca aattcaattg gatgttgaat tattttagaa gtttcttcta cagaatatcc
     1561 ctcagataaa tatcttctag ctataacttc catagactct cctatctcaa taatatgctt
     1621 atctgggtca taaatacgca ctactctttg ttgccattca tgttttagcg gcttatgaac
     1681 aaactctaca tctttataat aattgagttt ttcaataaat tcgtcaaaat catcaacctc
     1741 aaaataaagt tccatattat ttgatttttg tataattgta tctggattaa tccctgtaag
     1801 ccatgcgaag tcttcttgta tagcaaatcc tcctttaaat gttacatttt tacctaagtc
     1861 caattcaatt tcttgctcaa ataattcttt gtaaaatttt tttgatactt caatatcctt
     1921 cactgctaat aatgaaacct gatatttcat aaattttcct cctaattttt tatttctatt
     1981 ttttcagtat cttctattaa cattttttct ctatctcttc agtacctttt attaatcctt
     2041 tatttttact tttttagtat cttctattaa cacttttgtt tctacctcct tagtatctta
     2101 tatcaatctt ttattttttc aatatcttct attaatactt ttgtttctac ctcttcagta
     2161 tcttatatca atcttttatt ttttcaatat cttccattaa cacttttatc tctatctatt
     2221 cagtactttc tatcaatctt ttgtttttat tttttcaata tcttctatta atacttttat
     2281 ttctacctct tcagtatctt atatcaatct tttatttttt caatatcttc tgttaacact
     2341 tttatctcta tctctttaat atcttatatt aatcctttat ttttattttt tcaatatctt
     2401 cattaattcc tatttatttt atcatcttaa atacgaaaac actgtgtcat atttgcaaaa
     2461 tatatattat catctatatt aaagcttcac aatttatatt taaaaattct ctttaattac
     2521 actacctcta tttaaaaagc taccttaatt atacttataa gtaaaggtag ctttttaaat
     2581 ttaattaaat agaatatcca ctattaattt ttatttaatt tatttagatt cacattatag
     2641 tatcataatc cacttataat catttgtcat attacttaag tagactaata tattacataa
     2701 atacacttac atacacttta tattgatgct ataagaaata cgtttgctta tacgtctttc
     2761 tcctcttctt ttagattttt tatccactct tcaataagta aaatatcatc tttgctcaaa
     2821 ttaatatttt catgtaatga caacaataaa ctttttatag aagaatcatg aatatttcta
     2881 aaaaaatctc ttgtctcaac tcctatgtat tccttttctt taattaaaac ttcataatgc
     2941 gtatatttat ctatcttttg agaatttaga aatcttttta ctactaatct agataaaaga
     3001 gtcaaagttg tcgtttgttt ccattgatat ttttgctcca ttcctaaaac aatatctcta
     3061 gatgttactt tggaatctga tttccatata aatttcatta tctttaattc tgattgtggc
     3121 aactttttta tagtcatgat aaaaccttgc tactcagaat atagacaagc aatatccata
     3181 ataaaatttt tcgattaaat acatcttata cggatttata taagctcaga cttataagat
     3241 atttttcaat cttaatccta aatttagcta tttgtacacg gtaaatatat ataaataatg
     3301 tcaaaattct cttgtattat attgcttaat tttatgaaat attttgtact cagctatcta
     3361 ttttggatac ttgtccagta gctctccttt cttttaaact ctttgatttt aaatatttgt
     3421 atttcagtaa atcctacttc ctaaattttt tatttactac aacttattac tgaacttcct
     3481 acttcctaaa ctttttattt actacaactt attactgaac ttctcactta ctaaatttct
     3541 tacttactat aaattataac taaacttctc acttactaaa tttcttactt actataaatt
     3601 attactaaac ttcttacttt ctaaatttct tacttactat aaattattac taaacttcct
     3661 actttctaaa tttcttactt actataaatt attactaaat ttcctactcc ctaaatttct
     3721 tacttgctac aaattattac taaacttcct actccctaaa tttcttactt gctataaatt
     3781 attactaaac ttcctactcc ctaagtttct tacttgctac aaattattac taaacttcct
     3841 actccctaaa tttcttactt actataaatt attactaaat ttcctacttt ctaaatttct
     3901 tacttaccac aaattattac taaacttccc acttcctaag tttcttactt actactactt
     3961 ataactaaac ttcccacttc ctaaatttct cgcttactac cacttatcac taaacttcct
     4021 actttctaaa tttcttgctt actaccactt atcactaaac tttttacttc ttaaatttct
     4081 tacttcttaa gaataatgtt aattttaatt ttaagattat agtttttgcc ttgtttaatt
     4141 g
//
LOCUS       DAEMHW010000263         4135 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEMHW010000263 DAEMHW010000000
VERSION     DAEMHW010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.265"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            49..1743
                     /locus_tag="KPK67_003541"
     CDS             49..1743
                     /locus_tag="KPK67_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBG2460457.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYRIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2074..2184
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2288..2659
                     /locus_tag="KPK67_003542"
     CDS             2288..2659
                     /locus_tag="KPK67_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBG2460458.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2755..3702
                     /gene="trxB"
                     /locus_tag="KPK67_003543"
     CDS             2755..3702
                     /gene="trxB"
                     /locus_tag="KPK67_003543"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG2460459.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3806..4123
                     /locus_tag="KPK67_003544"
     CDS             3806..4123
                     /locus_tag="KPK67_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBG2460460.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT         1711 a          387 c          778 g         1259 t
ORIGIN      
        1 tctatattta taaatacaat aaagtaaagt tgtaaaggag tgctttaaat gaagttttca
       61 gagtttaagt atgaaagacc caattataat agtatgaaaa aagagttttt gagttgtgta
      121 gaagatataa ataattctcg taattataaa gagcaacaaa aaaatataca taagattaat
      181 ttattaagaa ataaaataga aacgttgtca aatatagctt cgataagata tagtacagac
      241 acatttaata aattctacaa agaagaaaaa aattattggg atgaatatat gcctttgtat
      301 gaagaattaa attcatattt ttataatgcc attgtaaatt caaaatttaa atatgatttg
      361 ataaaagagt ttggagagca atttttcaca atagtagagt attctttaaa aagtttttcc
      421 aaagaaataa taagtgaatt gcaggaagaa aataaattgt gttcagagta tactagactt
      481 ttagcttcag ctgagataat gtttgatggg aaaataagaa atttatctgg tatggggaaa
      541 ttcatgtatt ctaagaatag aaaaacaaga gaattagcaa acaaagcata ttataatttc
      601 tttgaagaaa atgaaactaa atttgatgac atatttgata aattggttaa acttagagat
      661 aagatgtcta aaaaattagg ttttgaagat tttgtagagc ttgggtacgt tagaatgatg
      721 agaagtgact acagagaata taggataaaa aatgttagaa aacaagtact aaaatatgtt
      781 gttccaatgg caaatgagct ttatgaaaaa caagctaaaa gaattggtct agaatattta
      841 agttacatag atgaaggcgt ggaattttta acaggaaatg caagtttaaa gggagattca
      901 agatacataa ttcaaaatgg taaaaggatg tattctgaac tgtcaaaaga aacaaatgaa
      961 ttttttgatt ttatgttaga aaatgaactt atggatttag aaacgaagaa aggaaaagga
     1021 gctggagggt attgtactta tataccagat tataaatcac cattcatttt ttctaatttt
     1081 aatcaaactg ctgatgatat agatgtgtta acacatgagg caggacatgc atttcagttg
     1141 tatatgtcta gatggattga tatgccagaa attaattttc caacgcttga tagttgtgaa
     1201 atccattcaa tgagcatgga atttataaca tggccgtgga tggatttatt ttttaaagaa
     1261 gatacagata agtacaaatt cactcactta tcatcttcaa ttaaatttat accttatgga
     1321 gtaattgttg atgaatttca acactatatt tacaaaaatc ctaatgtaga taagtcaaaa
     1381 cgaaaagaaa tctggagatt tttagagaaa aaatacttac ctcatagaaa atatggtgat
     1441 aattcttttt tagagagagg ttgttggtgg tttaaacaag gacatatatt taaaaatccg
     1501 ttttattata tagattatgt attagctcaa atttgtgcgt tacaattttg gaaaaaaatg
     1561 attcaagata gagatgcagg atggaaagat tatataaata tctgtaaagt tggaggaaca
     1621 aaatcatttt tagatattgt gagtatggga aatttgtatt ctccatttga tgatggctgt
     1681 attgagtcta ttataggtga tgttaaatct tggtttgatg aaataaatga cagcaaatta
     1741 taaaaaatta tgagaatttt attgtataaa agtgaaaaat taaaaaatat ataaaaagtg
     1801 attatttgtt gtgaaaatag tcttgttttt aactaaattg ataaaataaa ctaaaaaaga
     1861 tttgtactta aaaaaaaaag aaaatacaag aaatatgcaa tatcaacatt tggaaaaaaa
     1921 gtaaaaatgt atacaaaata catataaaaa aactattgca aattttatag aatgtttata
     1981 tacttaagtc aacagagata gaaaaaagct atttatagaa aacaaaggat aaattgctta
     2041 attttaaatt ttctgaaaaa aataataatc cggatgaaga gtatgggaga ctgctttata
     2101 aaaagcgacc gaagggacaa gtttaatctt acttacccaa aagattagat gaaattctca
     2161 ggtaaaggtg actgtacttg gatggggctc tgaaaatgat tttaacaggg aagacaaatt
     2221 gggagatttg tttttcctgt tttttattta tcaagtaata aataaaagtt tgaaagagag
     2281 taaaaaaatg ataataatta cgaataatcc aaaagtaaaa gaagaagttc aaggcagaga
     2341 agttttgttt aaagacacaa cttatattgg aatcttagaa gcaagtagag atttgataca
     2401 tgaaggttat gaacttttat ctcatccact ctatgggagt gtaaagccaa atgaaacacc
     2461 ttacaggact gttatactaa agaaaggaaa tcgtttggat ataaactctt taaccttaat
     2521 tgaagaagca atcatcactg caagtaaatt tcagaataat aaaaagactc caaaatggac
     2581 agaaagtgtt caagatgatt ttagagtaat tgattatgat atattttata atacaataca
     2641 aagaatgcag tatgaataaa aaaaattgtt aaaaagttat taaaacagcc taaatattaa
     2701 atctaattta atattaaata aaatgagaaa tttaattggg gaggataagt caaaatggaa
     2761 aatgtgtatg acttagtaat aattggttca ggaccagcag ggcttgcagc aggtctttat
     2821 ggagcaagag caaagttaaa aactctaatc ctagaaaaag acaagactgg tggacaaatt
     2881 gtaataacac atgaaattgc aaattatcca gggtcagttc caaatgcaac aggaccaagt
     2941 ttaatagcta gaatggttga acagtgtaaa gagtttggag cagaaatgct aagagacaac
     3001 attgttgata ctgagctaga tggagatata aaagttttaa aaggggaaaa ggcagaatat
     3061 agagcaaaag cagtaataat aggaacagga gcaacaccaa gaaagattgg ttgtcctgga
     3121 gaaaaagaac ttactggtaa aggggtttca tattgtgcaa cttgtgatgc agatttcttt
     3181 gaagattttg aagtatttgt tgtaggtgga ggagacagtg cattagaaga agcaatgtat
     3241 ttaactaaat ttgctagaaa agtaacaatt gtacatagaa gacaaggatt tagatgtgct
     3301 aagagtgttg aagaaaaagc aaaagctaat ccaaagatag agtttttatt agatacagta
     3361 attgaagaaa tcaaaggtga tggaatatta gaatcagttg tatttaaaaa taaagttact
     3421 ggagaaactc atgaatactt tgcagatgaa gaagatggaa caatgggagt atttgtgttc
     3481 gttggattag atgctcaaac tgatttattc aaaggtaaag ttgatatgga tgaaaaaggt
     3541 tacataataa ctgatgaaga tatgagaact aacataccag gcgtatttgc agcaggagat
     3601 tgtagatcta agactctaag acaggttgta actgcaacta atgatggagc aattgcatct
     3661 atagtagctg agaaatacat tgatgaaaaa tttgggaact agaatctgta aattaagaaa
     3721 tagtgtctgt aaatttaatg aataatattt aaaattaaaa ttaatattta aaattaaaat
     3781 aaaaatttca agggggaata agaaaatgtt agatttagat aaggctacat ttgaagaaga
     3841 agttttaaat gcagaaggtt ttgtatttgt tgatttttgg agtgaaggtt gtgagccttg
     3901 taaagcttta atgccagatg ttcataaatt agctgagact tatggggata aaataaaatt
     3961 ctgtaagatg gatacaacaa aagctagaag attagctata aaacaaaaag tattaggtct
     4021 tccaactatg gctatataca aagatggaga aaaagtagac gaagttacta aggatgatgc
     4081 tacagttcct aatatagaaa acatgattaa aaaatatctt taatatgttg ataaa
//
LOCUS       DAEMHW010000264         4116 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEMHW010000264 DAEMHW010000000
VERSION     DAEMHW010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.266"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            303..1175
                     /locus_tag="KPK67_003545"
     CDS             303..1175
                     /locus_tag="KPK67_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460461.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            1172..2461
                     /locus_tag="KPK67_003546"
     CDS             1172..2461
                     /locus_tag="KPK67_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460462.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDNILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWFYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2391..3035
                     /locus_tag="KPK67_003547"
     CDS             2391..3035
                     /locus_tag="KPK67_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460463.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMSKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3302..3766)
                     /locus_tag="KPK67_003548"
     CDS             complement(3302..3766)
                     /locus_tag="KPK67_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2460464.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
BASE COUNT         1562 a          484 c          611 g         1459 t
ORIGIN      
        1 aaggctaaat accctatctt attatatcca gtaattcttt tggatactta tcacattttt
       61 cattatatat aaaaatagac tcacaaataa aataaatata tattaaatta taacttcata
      121 aacttaaaaa atttcagaaa caaagatact ttaatttaat taaaaacaga aaaattaaaa
      181 tctttagaaa aaatttagaa atttataaat aatatcttaa gttttagaat ttattatgaa
      241 aatacagtaa aaatttataa aaataaaatt ctataaaatt gtttctgggg gtgtatttat
      301 atatggaaat gaaaatagca gtatccaatc ttagcaaaag atttgggaaa aaacaagcac
      361 ttgataatat aaatcttgaa atcgacagtg gtatgtttgg tcttttagga agaaatggag
      421 cagggaaaac cacatttatg agaattttag ccactttatt atcaaaaaca gaaggttcaa
      481 tacatatgtg tggagttcct attgagaata ctaaggaaat tagaaatatg gttggttatt
      541 tgccacaaga tttttcaatg tatcctaaca tgacagtata taaagcaatg gattatttag
      601 cagttttatc ggatatacca ctaagaaaaa gaagaaaaat tatagcaaag ttattaagca
      661 aagtaaacct tactaattat caaaatgtga aagtaaaatc tttatctgga ggtatgaata
      721 gaaggcttgg gatagcacaa gcattaattc ataatcccag agttttaata gtagatgagc
      781 cgacagcagg tcttgaccca gaagaacgta taagatttag aaatttacta tgcgaaattg
      841 caaatgatag aatcgttatt ttatctacac atattgttgg agatatagaa gctacatgtg
      901 aaaacgtagc tatattgaat agtggaaaag tcatatacaa tggctcaaca gaagcattag
      961 caaaactagc tgaagggaaa gtatatagta ttgaagtaga taaaaaagac attgaaaata
     1021 taaaaagtag atttatagtt ataggaatgt tgactcatgg tggaaaggct atacttagga
     1081 ttatatcaga tgataaacct tttgagacag cagtaaattg taatccaact attgaagatg
     1141 gctatatgct tataatggga ggagataata tatgaatcta tttataaaag aatgtaaaaa
     1201 tatattaaag agtatggtat attatatttt tattgtagtt ttagtttttt tctatatttc
     1261 tcaacttgga agttcagttt caaatgatgt aagtaaaaat attcctaatg atggaaatcc
     1321 attagttgct ccacaaaaag tagagaatat tgaggaatat tactctgtac acaatcaata
     1381 tccatatggt caaaaagaat ctatgaaaac agaaaaggtc attccaaatg caactataaa
     1441 cctaattaga gaatatcaaa aaaacacata tacaacgtat ccaacagggg tagttaagta
     1501 tataaaatta gaccaaaata aaacgaagac catagaaaaa tatatagaag aaataactgg
     1561 taaaagtatt agcgaaataa attcactttg gaaaactgat gaaagtataa aaatagatta
     1621 ttctaaagta tctcttgaaa gatttgaaga aataatgcaa agagtagaca acattttagg
     1681 aggagggtct gcatatgctt ttgacaagtt acaaagattt ggagtagttc cagttaccta
     1741 tgatgaagct atgaagaatt acaactatac aatacaaaaa gataaagtta gtaatgggta
     1801 tgcaagaatt ttttgcgatt atataggtat agttttaagt atatttccag tatttgtggc
     1861 agtttttatg tcacttaaag ataagcgttc aagaatgaga gagattattt attcaaaaca
     1921 agtatcttca tgcaaaatag ttttatcaag atattttgca cttgtcttta tgatgatatt
     1981 gcctgtatta attttaggta tgaaagattt acttcctttg attttctttg ctaaagataa
     2041 aggtatatct attgataact tagcatttat aaaatataca ttatggtgga tactaccaac
     2101 tcttatgata gttaccgctg taggaatgag tattactata ttaacagata caccagtagc
     2161 aattattgtg caattaattt ggtggtttta cagtataaat tctacaggac tcaaaggtga
     2221 ttattcaaca atgggactaa tgataagaca taatagtgta ttaaatgggg atattattat
     2281 acaaaacttc aatcaaatta tgattaatag aattgtgata agcttgggag cattggtgct
     2341 tgttttgtta gctatattct tatatgagca aaaaagaagg gggaatcttg atgtttgggc
     2401 aaagttttca aaactgttta gttctaataa aagaaaactt caaattaagc actttaacta
     2461 atttaatatg ttcaattata ttgctatgta tgattcctct aatacaagga gtagctaatt
     2521 tagacagaat ttcaacagct atatgtttag aaaattatgt tgcaataata gggatagtaa
     2581 tgttggtacc cgtattttcg ctagagcaat caaaagaaat tgatgagata gtatcttcaa
     2641 aagcattatc tcaagttatt gtacaattaa ttaggatact gatgtcaatt ataggtatag
     2701 tggtattaat cataggtttt gtaaatgttt taatatatct tggatgtaat tttccagtag
     2761 ttgagtatat tattggaagt ttcgcatcag ctatgtttat aggaagttta ggaatgtttt
     2821 tctcaaattt atttaataat acaatcatag gttatatgat atctatagga tatttaattt
     2881 taaatatgat gtctaaaggc aagtatgttg gaaaattttt tataatgtct atgagtcaag
     2941 gtagttttga tgagaaatat tggctgatac ttggaagtgt tgtgttgata attgcatctc
     3001 tcttaataaa accagtaaaa aggaagatta gataataaaa tgagagctta gataaaataa
     3061 aggtaatgca aataaaaagt acatttatga aatataaatc tatttttata tatacaaacc
     3121 tacttgcaat ctctttatgt atgtagatag atttatatat ccgtagtata aaatatatat
     3181 gtataccaaa ttttatcaaa agctctcaaa tttatatttc aaagacactt attgctatga
     3241 cctacacata tgtatttttt cattttaatt gttgtattaa caataggact attgttataa
     3301 actattgata cctcataaca aaaaattctc catctcctaa atgcgttttt tgtaccttag
     3361 tctcaactat tttaaatcct tgagactcat aacattttac agctctcatg ttccaggttc
     3421 ttacatttaa tacaattggt ttagtactat atttatttct acattcttgt aatcctaggc
     3481 taattatttc tttaccaatt cccattccac aataattcgg tttcatacca ataccaaaga
     3541 atacagagct tccttcatct aataagttta taaaacctat caattcttta ttgtcattta
     3601 taaaacttat aaaatttttt cttttatctt cttttgctag tgaaaaattc ttctttttaa
     3661 tttcctccca tggaggtaga ttgtatattg aatatttacc ttcatatttc caattagtaa
     3721 cttcaatagc atgttcttca gtaaatttaa ctttatttaa attcatatgt cctcctatta
     3781 aaatatttaa ggtaattaat ggtattttaa agttttagaa aataagtaaa actatttata
     3841 tttatccata aaatctttaa aaattttact aacaattctt gtaccctgta ctttttattg
     3901 gattcacaga agactataaa ctaattttga attaccttct aattcaaaaa catcacgata
     3961 tatataattt cttagcttga ttaaattctg ttaaatccgt ttcttatagt tgtataatca
     4021 acatcaatat ctttatgtgt attagttaat ttattggctt taggaactta ttattaatat
     4081 attgtatttg ctccaatact ccaagtagta ggtaaa
//
LOCUS       DAEMHW010000265         4105 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEMHW010000265 DAEMHW010000000
VERSION     DAEMHW010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.267"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            163..2262
                     /gene="nrdE"
                     /locus_tag="KPK67_003549"
     CDS             163..2262
                     /gene="nrdE"
                     /locus_tag="KPK67_003549"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBG2460465.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGNKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2275..3246
                     /gene="nrdF"
                     /locus_tag="KPK67_003550"
     CDS             2275..3246
                     /gene="nrdF"
                     /locus_tag="KPK67_003550"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBG2460466.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3322..3948
                     /locus_tag="KPK67_003551"
     CDS             3322..3948
                     /locus_tag="KPK67_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG2460467.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIIEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTIFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1640 a          451 c          766 g         1248 t
ORIGIN      
        1 taaagcactg actatatgta gtgcttttgt ttttgaaaaa tacttattgc atttctataa
       61 ttatagatta attttgggat ttcaactgat aagtaccaaa agaaataata agcaaattaa
      121 gtgaaattat ggcacaagat tatgttaaaa ggacggtaaa taatgaattg gatagagctt
      181 aacaatcaag tgataattaa aaatgaacaa ggcaagtacc aattggaaaa ggataaggaa
      241 gccttaagta gttacataga agaatttata cagccaaagt tgagaaaatt taataaccta
      301 gaagaaagat tgaagtattt aatagatgaa ggttattatt caaaagaagt aataaatcag
      361 tatagcatag atgttattaa aaatatatat gatatgattg ataaacataa gtttgaattt
      421 caatcatata tgagtgcaaa taagttttat caaaattatg cattaaagag caatgatgga
      481 aaggaaatat tagaaacata taatgataaa gtgttaattg tagctttgac actaggaaat
      541 ggtaataaag atttagcttt aaacttggca gataaaatag taaaacaaga atttcaacca
      601 gctacaccta cttttttaaa tgctggaaga aaaagggctg gagaaatggt atcatgtttt
      661 ttactatctg tagaagatag tacagaaggg atatcgtatg ctatatcttc atcaaatcac
      721 ttatcaaaga ttggtggagg agttgcacta aatttatcta aacttagagc atctggcgaa
      781 tctatcaaag atatagaagg tgcagctgga ggtgttgttg gagtagctaa aatgttagaa
      841 caatcattta gttactttaa tcaaatggga gctagacaag gttctggtgc ggtgtacctt
      901 actgtattcc atcctgattt tgaattgttg atggatacta aaaagataaa tgcagatgaa
      961 aaaataaggc ttgctacatt atcattagga gctataatac cagataaatt tatggagttg
     1021 gcagaaaaaa atgaagtagc atatgcattt tatcctcata ctgtgtataa gaaatatgga
     1081 gtttctcttg atgagataga aatggatgag tggtatgata aattggttaa cgatagtgat
     1141 ataagaaaaa aagaaataaa tcctaggcag atgttgacaa aaatagcaca gatgcaacaa
     1201 gaaagtggat atccatatgt agtgtacata gatacagcta atagagaaca tacattaaaa
     1261 gatgttggaa ttattaaaat gtctaactta tgttgtgaga tatttcaata tcaaacacct
     1321 tcagaaatag aggggtatgg aggaaaaaat gaatggggac aagatataag ttgtaaccta
     1381 ggttctttaa acatagccaa tgtaatggat aataaaacta tagaaagtac agttgaaaca
     1441 gcaataagag ctctgtcatt tgtagcagat agcacagata taaaaccagt gccaacggta
     1501 agcaatagta acagcaagtc tcattctata ggattaggag ctatgaattt acatggatac
     1561 ttagtaagag aaaatatttt atatacttca gatgatgcta tagatttttc taatgtattt
     1621 tttgcaatgg ttagatacta ttcaattaaa gcttctatga aaatagccat agaaagaaat
     1681 caaacttttg aaggatttga taaatcagaa tatgttaagg gaagaaatag caaggtttta
     1741 tcaaaatact atgaacaatc ttatctacct aagtcagaaa aggtaagagc attatttgaa
     1801 gggatttata taccaacaaa agaagactgg acaaaattat tggatgaggt aaaagaaaaa
     1861 gggatatata atgcatattt aatggcagta gcaccaactc aaagcattag ttatgttcaa
     1921 aatgctacat caagtataat gccaattact gagccagtag aagtaagaac atatggagac
     1981 tctactacaa tatatccaat gccattttta acaaatgata atatgttgta ttatcaatca
     2041 gcatacagga tggatatgag aaaggtcata gatttagtgg caacagttca aaaccatgtt
     2101 gaccaaggta tatcaacaac actgtttgtt acagatgaaa agacaactag agatatagct
     2161 agacattaca tatatgccta taaaaaaggt ttgaaaagtt tatattacac aagaacaaag
     2221 atgacaagag atactcatga gtgtctagtg tgttcagtat agggagagaa atatatgaca
     2281 tttaatttac ataaaataca taatgcagtt aactggaata gagaagaaga tggatttaca
     2341 caagcttttt gggaacaaaa tgtaaaacaa ttttggcttc ctgaagaaat atctgtttct
     2401 aaagacataa aggtatggaa tgaactaagt aataaagaaa aggaattata taaaaaagta
     2461 ttgggtggtt taacattact ggatacaaaa caggggaata atggaatacc atctatgatg
     2521 agtttaacag aaaacctaca aagaaaagct gtcttgtctt ttatggggac tatggaagaa
     2581 atacatgcaa aaagttattc ttctatattt atgactctac tttctaattt agaaatagat
     2641 gaattatttg aatggataga aacagaacca acattacaaa gaaaggctga tttagtttta
     2701 gctcaatacg aaaatactac aaatcaagag ggattatacc tatcaatggt tacaagtgta
     2761 tttttagaga gtttcttgtt ttattcagga ttcttttatc cattgtatct atctggtcaa
     2821 ggaaagatgg tagctagtgg agaaataata tcgcttattc ttagagatga gtctttacat
     2881 ggtaagtata taggattgct agcacaagaa atatacgatt catttgataa aatggataag
     2941 gagatgttag aagagaagat gtattctata ctttatagcc ttatggagaa tgagatagag
     3001 tatacaaatg ttatatacag agaaagtggg cttgaaaaag aggttgtaaa ctttttaaaa
     3061 tacaatgcga atagagcttt agaaaatcta gggtttgaga agctatatac tgtagatgct
     3121 ataaatccaa ttgtattaaa tggtcttagc actgaaacca aaacacatga tttcttttca
     3181 actaagggta atggatacca aaaaggagtg tatgaggaat tagaggatga agattttata
     3241 atataattta taattatgac acactgttgt cacttttagc gtgataatat caatgtataa
     3301 aatatatatg gaggtatttt tatgaaatat gaatggagaa aaagagataa agagttatat
     3361 ttacctaaaa agaatcctgt aataattgaa ataccagaac agaaatttat catgttaaaa
     3421 gggagtggag acccaaattc taaagaattt actgaagcaa taggagtact ttattctttg
     3481 gcatatgcaa taaaaacaat gcctaaaaaa ggaattgtac cagaagggta ttttgactat
     3541 acgatttttc ctttagaagg tatatgggat aagggagaaa aaagtaaagg cttagatgta
     3601 cttattaaag aagatttaat ttacactata atgatacgtc agccagattt tgttacacaa
     3661 gacctgttgg ataaagctac tgatatagta aagaagaaaa aacctcataa tcttcttgaa
     3721 aaagttagat ttgggtctat taatgaaggg ctatgtgttc aaatgcttca tataggttca
     3781 tatgataatg aaccagaaag ctttgacatt atgaacgaat tttgcaaaaa aaataaactt
     3841 ataagaaaat cttacacaca tcgtgaaatt tatataagtg atgctagaaa agtatcgcct
     3901 gaatcattga agacggtttt acgttttgaa gtagaaaggg aagtataaat attttgaata
     3961 atttataaga gttataaaaa gtgaagtctg ttgagacttc attttttgat gcaattttaa
     4021 aattaaattt gatgtttcat aatattttaa aaactttcta cttttattag tgtgaataac
     4081 tgttgctatt aaataataat actaa
//
LOCUS       DAEMHW010000266         4046 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEMHW010000266 DAEMHW010000000
VERSION     DAEMHW010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.268"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            281..1771
                     /locus_tag="KPK67_003552"
     CDS             281..1771
                     /locus_tag="KPK67_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2460468.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            2051..3547
                     /locus_tag="KPK67_003553"
     CDS             2051..3547
                     /locus_tag="KPK67_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG2460469.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3681..4031
                     /locus_tag="KPK67_003554"
     CDS             3681..4031
                     /locus_tag="KPK67_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG2460470.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNDVSQNNIQSSTKNTAFKTTILSTGKSDCILIEIGDKFIMIDTGEDKNGEQIVDKL
                     KEKEINALDYLIKK"
BASE COUNT         1631 a          407 c          773 g         1235 t
ORIGIN      
        1 agataataaa tttaatatca aaaaatgtaa caaaaaggta tgtaagtaaa aagtaaaatt
       61 aaaatatata ataaaaaata ttatatatta gattgaaaag taaaaataaa aatagtgaaa
      121 tttcagaaat atcaataata ttatataata aaacaaaaaa acactatata tattcgataa
      181 aataaaattt aaacatataa tattttatgt atatattaat aaaaggacat attgtgttat
      241 aatggtatcg tataaaatca tttacggggg tggtattaaa atgtttcaat ttgaaacaca
      301 aatacacaaa ttaagacatg atgttttaac tgcaatagct agacttggaa aagaagataa
      361 ccttaccaga gaagcaatgg ataatattca gtatgaaata ataaaaggtg agaaacctac
      421 atacagatgc tgtgtttata aagaaagagc tataatatct gagagagtaa atgtaagtat
      481 ggggttacaa ccaggtcaag ataagataga tttagataca atggatatag aagaagatga
      541 acaaattata tatattttgg aagctgcatg tgacagttgt ccaataaatg aatttacagt
      601 aacagatatt tgtagaggat gtctagcaca tagatgtaaa gaagcttgta aatttggagc
      661 tataacacat gtcggaggaa tggcctatat aaatcatgaa ttatgtaagg cttgtggaat
      721 gtgtaaaaaa gcttgtcaat atgatgccat ctcagaggtt gttagaccat gtaagagtgt
      781 ttgtccaact aatgccttag gatttgatag agaaaatatg aaagctatga tacatgagga
      841 taaatgttta aactgtggag catgtatgtc agcatgtcca tttggagcaa tatctgataa
      901 gagtttgata gctcctgtag ctagaaagtt agttcaaaaa gagaaaatgt atgcggtggt
      961 agctccagct ataacaggtc aagttgaagc aaatgttaca tatggtcaaa taaaaaatgc
     1021 tataaaatca ctaggatttg ttgatgtatt agaagcagct tgtggagcag atgcagttac
     1081 agttcatgaa agcttagaat ttgctgaaag aatggaaaag ggagaagctt ttatgacaaa
     1141 ttcttgttgt ccaggatttg ttggttatat agaaaaaaca tacccaaatc atgtaggaaa
     1201 aatatcttct acagtgtctc ctatgatagc tagtggtaga tttataaaaa gtcatgaacc
     1261 agatgctaaa gttgtattca taggaccatg tacagccaaa aagagtgagg cagctaagaa
     1321 agaattaaaa ggagctgttg attatgtact tacttttgaa gaaataatgg cattatttga
     1381 ggcatttgat atagacttgg ctaagtgttc aaatgaagat atagatgatg catcaatttt
     1441 tggaagaggt tttggagctg ctggtggtct tacaaaagct atagaaaatt atcttgctga
     1501 aaaaggtatt ggtgttaatt ttgaacctgt aaagatttct ggttcaagag aaatcaagaa
     1561 aactatgact atggctaatt taggaaagct agaaggtaat tttatagaag gaatgatgtg
     1621 tgaaggtggt tgtataaatg gttctggtaa attcttatca ggtgcaaagg ttaaagccac
     1681 ttttgataga aaaaattctc aatcaactaa gaaaagtgtc ttagcaaata caaagataaa
     1741 agattttagt aatataaatt tagaaaaata aagataatac aaagcaacaa aaatacatat
     1801 tgttaataga gttgttctaa gttgaggttt aacttttgga caactctatt tttatttata
     1861 tatttaatta ttgaacaact aaaataaaaa ttatatacac ttaaaataaa aattacatat
     1921 ataatacttt gtatataact taacaaagta tatgcctatg ttataataat ttatagaaat
     1981 aaataaaaaa tacaatatac ataatagtag aaatatagca aaaaggtgtg atttataggg
     2041 aggattactt atgtttgaat ttgaaacgca actaaaaagg ttaaaacatg aagttttaac
     2101 agcagtagca agactatcta ttgaagaaaa tttaacaaaa gaaaatatag aaaaaattcc
     2161 atatgaaata attaaaggag aaaaggcgag atatagatgt tgtgtttata aagagagaga
     2221 agtagttctt gaaagggcgc aacttgcatt aagtttaact ccaaatagta aatatggaga
     2281 tataaatcca gattcagtta gtttggacac agatgagcaa attatatata tagttgaagc
     2341 agcatgtgat agatgtccaa taaatgaatt tacagttaca gagctttgta gagggtgttt
     2401 ggctcataga tgtaaggaag catgtaaatt tggggcaatt tcttatataa atgggagagc
     2461 atatataaat catgatttat gtaaggcttg tggaatgtgc aaaagttcat gtcaatatga
     2521 tgctatatca gaagttgtaa gaccttgtaa aagcgtttgt ccaacagggg ctttggattt
     2581 taatagaaac actatgaaag caatgataca tgaagataat tgtataaatt gtggagcatg
     2641 tatgtcagca tgtccttttg gagcaatatc tgataagagt ttgatagcac ctgttgcaaa
     2701 aaaacttgca aagaaagaaa atatgtatgc aatagtagct ccagctataa caggtcaaat
     2761 agatagtaat attacatatg gtcagttaaa aaatgctata aaatcactgg gatttgtaga
     2821 tatgatagaa gcttcctgtg gagcagatgc agttacagtt catgaaagca atgagcttat
     2881 tgaaagaata aaatcaggag ataattacat gacaaattca tgttgtccgg gattttttaa
     2941 gtatatagag aagatgttcc cagatgaaaa agatagaata tcatctacag tgtctcctat
     3001 ggtagcaaca ggaagattag taaaaaaaat agattctgat gctaaagttg tatttatagg
     3061 tccatgtaca gctaaaaaga gtgaggcaac agagaaaagt ttaaaagatt cagtagatta
     3121 tgtacttact tttgaagaac taatggcatt atttgaagca tttaatatag atttatcaaa
     3181 atgttcaaat gaagatataa atgatggttc gatttttgga aggggatttg gagcatctgg
     3241 tggacttgcc aaatcagtac agaactatat tgctgaaaag gggcttagtt ttgattttga
     3301 acctataaaa gtatctggtc caagacaaat taaaaagaca atgacaatgg ctaaattggg
     3361 aagattagaa ggtaatttta tagaaggaat gatgtgtgaa gaaggttgta ttaatggagc
     3421 aggtaagttt ttatcaggtg taaaagctaa aaatactttt gatagaaaaa atggacaatc
     3481 tactaaaaag agtgttttat ctaatgataa gattaaagaa tttgaagatg taaatttaga
     3541 aagataattg ttttggtaca gtcatttttt tatcatgcca caaatatgcc taaaatttgc
     3601 cctttttata tgtgtatttt acagagttaa ggttgctata ctataaatat gtaataaata
     3661 aaatttatag gagcttttgg atgaggaaaa tagtattaaa agatatcctg tttttagtta
     3721 ctacattatc tataatagta atattattgc ttgtaacaaa tataactact aatagtaaga
     3781 taaaatcaac aagtgaatct tatttaaaaa gtgacaaaaa tgatgtgagt caaaataata
     3841 ttcagagtag cacaaaaaat actgcattta agactactat attaagtact ggaaagtctg
     3901 actgtatatt aatcgagata ggtgataaat tcataatgat tgatacagga gaagataaaa
     3961 atggagaaca aatagttgat aaattaaaag aaaaagaaat aaatgcactt gactatttga
     4021 ttaaaaaata gctacgcaca ctcgtg
//
LOCUS       DAEMHW010000267         4038 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEMHW010000267 DAEMHW010000000
VERSION     DAEMHW010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.269"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            12..410
                     /locus_tag="KPK67_003555"
     CDS             12..410
                     /locus_tag="KPK67_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2460471.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVK
                     INLNIKGYKFLPYVKNLKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            398..1336
                     /locus_tag="KPK67_003556"
     CDS             398..1336
                     /locus_tag="KPK67_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG2460472.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1720..1944)
                     /gene="secG"
                     /locus_tag="KPK67_003557"
     CDS             complement(1720..1944)
                     /gene="secG"
                     /locus_tag="KPK67_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBG2460473.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2549..3841)
                     /gene="eno"
                     /locus_tag="KPK67_003558"
     CDS             complement(2549..3841)
                     /gene="eno"
                     /locus_tag="KPK67_003558"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBG2460474.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1388 a          646 c          455 g         1549 t
ORIGIN      
        1 aaggagttgc catgataaaa aaaatagaac atgtggtcat tactaataat aatatagaaa
       61 attatatcaa tttttataaa aaatttggtt tttcatctaa agaaatgagc aaaaattata
      121 aacttaatgc tagaaatgtt aagataaatc ttaacataaa aggttataaa tttttacctt
      181 atgttaaaaa tttaaaagca agtagagata acttatgttt taaaataaac acaaatattg
      241 atgagccaaa aaaatactta gaacaaaatg agattcaaat taagcgagga attgtagaat
      301 gtacaggtgt ctttggtaat atgaaatcta tttatttgaa aaaaactaat ggaaatttaa
      361 ttgaattatc aaatcataga atcacggaga gtaaaccttg gaaaatttaa tcctatcaat
      421 aaatgttgtt cttcctttgt ttctcacaat gtcacttgga tatgttctaa aaaaattagg
      481 tctatttgat actcatactt taaataaaat gaataatgtc tgcttcaagt ctttcttgcc
      541 attattatta ttttttaata tatacaatac agatttagat ggttctctta attttaagct
      601 tatggctgta gctgttataa ttattttttc aatcttctta atcttagtct ttattattcc
      661 taaaattgaa aaagataata agacaagggg tgcattaata caaggtgtat ttcgtagcaa
      721 tttcgttata tttggtattc ctgtgacaat ttcactattt ggcgataaga gtattggagt
      781 cacatcttta ttaattgcag ttattgtacc tatgtttaat gtattatctg ttgtaattct
      841 tgaaattttt agaggtggca aagtaaatat aaaacatatg attaaaagcg tcttaacaaa
      901 tccactcata atcgcctctt ttataggatt gacaatgcta attttaaata ttaatatacc
      961 tacattttta cacaaaacta ttggtgatat ttctaaaata gctactcctt tatcacttat
     1021 aattctaggc ggctctttta gtttttctgc tataaagggg catataaaac ccattatatt
     1081 gggcgtatgt ggaaaactta tacttgtacc tgctatattt ttaccaatta gcatttttct
     1141 tggatttaga aatgtagaat tagcttcact tatgataatg ttatctgctc caactgcagt
     1201 ttcttcattt acaatggcac aacagatgga ttctgacagt gaattagctg gacaaattgt
     1261 agtatttact tctggatttt ctataattac agtattttta ataatattta ttttaaagca
     1321 gaataatctt atttaatgat atttgataac acaaactggt attatgagaa acatatataa
     1381 aattgattta attgcgtaaa aacatattat tatactattt ttactatata atataacttt
     1441 gtgtatatta tctcattaaa catggtgaaa gtagtgttgc tatatatttt ttagtttgaa
     1501 gaaaatatat aaatacaaac aatatataaa tacaaataat aaacgccaac aaatagtcaa
     1561 tctataaaat aaaaactgga ctaatcatat aaaatcatta gtccagcttt tattttacgt
     1621 actttttatg ttgatgtact tttattaaaa actaaattta atttttaaat gtctagcaaa
     1681 tgcactaatt aaataatggc cagttattca acaatatcac tacttcttaa ctataagtaa
     1741 tgccaatgca tttataaaaa ataatactgc tgttatcttt gtaactctag caagaaatgc
     1801 ttgttttcct tttggtttaa agtaagcttg acttccttct ccaccaaatt ctgaaggtaa
     1861 agcattctta gaatcttgag gcattatgct aattactagt aaaatcccta gtactacttg
     1921 cacacccatt aaaatgttgc tcattcttgc ccctcctatc tgtttattac ttaaatttca
     1981 ttactcttta gtatatcaca ataatatagc catttcaaga ctattatgct ataaataaca
     2041 tacaaaaaaa tatattgtta ctataatata ttactatatt ctcaatataa aaatgaataa
     2101 aacttagaag agaaaactat ttaaattaat acataaatta atacataaat taacacttaa
     2161 aataatctgc tctcattgca tatattataa atagccacca aaataaaaca atcattttaa
     2221 aatattgata taacttttaa tttcaacata atttaaaata aataacccac atttagcaat
     2281 aatactttaa tgacaattat gattaaatca aaatatgtct tcacttttat tttttaataa
     2341 aaacctccta tgcaattaga taaaaatgag atagaaacat ttattcatat tttttctgaa
     2401 tctatgaata aaattaagta ataaaatata aaaaaggata agtgaattga aaaatcaatt
     2461 aacacttatc tcttttatat cttataagat gagcttaaat atcttatatt ctctcttcat
     2521 taatcttttt tagtaaccat tttaataact atttttttaa gttatagaaa gattttaatc
     2581 cacaatatct agcttgctct ccaaccattt cttctattct taataattgg ttgtacttag
     2641 caactctatc agttcttgat ggagcaccag tttttatttg accagcattt actgcaacag
     2701 ctaaatctgc tatagtagaa tcttcagttt ctcctgatct gtgagatata acagcagtat
     2761 atcctgctct cttagccatt tctatagcat ctaaagtttc agttattgta cctatttgat
     2821 taactttaac taatatagag ttagctactc cattttcaat tcctttttct aatctctctg
     2881 tgttagtaac aaataagtcg tcaccaacta attgtaattt attacctaat ttttcagtta
     2941 ataatttcca tccatcccaa tcttcttcgt ctaaaccatc ttctatagtt attattggga
     3001 agttgtttga ccaatcttca tataaagcaa ccatttcagc agcagttaac tcttttcctt
     3061 ctcctgctaa aacgtatttt ttagtttctt tgttgtacat ttctgtagca gcaacgtcaa
     3121 gacctaacat aacgtcttca cctggcttat atccagcttt tgttatagct tcaactatta
     3181 attctaaagc ttctctgttt gaacctaagt ttggagcaaa tccaccttcg tcacctacac
     3241 cacaagctaa tcctttttct cctaaaactt tctttaatga atggaatact tctgctccca
     3301 ttcttaaacc ttctttgaaa caacaagctc ctactggtaa tatcataaat tcttgaacat
     3361 caacgttatt atcagcatgc tctccaccat ttaatatgtt catcattgga acaggtaatt
     3421 gtttagcatt tactccacct aagtattgga ataatggtaa acctatttca tcagcagctg
     3481 ctctagctac tgccattgaa acacctaata tagcattagc tcctaattta cctttgtttg
     3541 gagttccatc taattctatc atgattgcat ctatagctgg ttggtcaaat gcatccattc
     3601 cttctatttc aggtgctatt atttcattta cgttagcaac agctgtttct acaccttttc
     3661 ctaagtatct tcccttatct ccatctctta attcaacagc ttcaaaagct cctgtagatg
     3721 ctcctgatgg aactattgct cttcccattg ctccatcttc taatactact tcaacttcta
     3781 cagttggatt tcctcttgag tctaatactt ctctagcata tactaattca ataactgaca
     3841 tttgtatatc tcccttcgat tttctattag ttaaattttc atttactgta aacatccgtc
     3901 tactatttta atctactttc tatattatag tacataaagt tgcttttgaa aattaggtac
     3961 aaatttattt gatgggtaaa tttgtaaaag ttcctatttt tattttattt tttataatat
     4021 tttaatttat ataatata
//
LOCUS       DAEMHW010000268         4016 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEMHW010000268 DAEMHW010000000
VERSION     DAEMHW010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.270"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..430
                     /locus_tag="KPK67_003559"
     CDS             <1..430
                     /locus_tag="KPK67_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460475.1"
                     /translation="QGYSHRINDLLSINSSTFLLIGFLVVLFFIAVGSILYFNNISAI
                     SDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFATLVYKTALMQNL
                     LGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            574..2157
                     /locus_tag="KPK67_003560"
     CDS             574..2157
                     /locus_tag="KPK67_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG2460476.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRKNNIISLFCGLLALDSWYLLLSFVTTPIGTIVFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLIF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDTTKHKGIISIKEQVENLGGAINISDNIPHGV
                     CVQITIPMKGDGSYKHFVS"
     gene            2129..2749
                     /locus_tag="KPK67_003561"
     CDS             2129..2749
                     /locus_tag="KPK67_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2460477.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYKEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGGTCFPSENTEVIIEDLTSMEKKILQLICSGKKRK
                     MIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(2938..3864)
                     /locus_tag="KPK67_003562"
     CDS             complement(2938..3864)
                     /locus_tag="KPK67_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG2460478.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNTYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         1420 a          558 c          484 g         1554 t
ORIGIN      
        1 acaaggatat tctcacagga taaatgattt attatcaatt aatagttcta catttctgtt
       61 aatcggattt cttgtggtat tattctttat tgctgttgga agtattttat actttaacaa
      121 tatatcagca atttcagatt caacatctga ttataagatt ttacagaaaa tgggttatga
      181 taatatacaa attaaaaaaa ttattaaaaa acaagtattg ccatttttta atattccgtt
      241 tttacttggt atattggact gtatatttgc taccttggtt tataagactg ctttgatgca
      301 gaatttactt ggaaattcat tttcacaata catacctgtg attattgcaa ttttcttaac
      361 ttctgtagtg tatttgatat attatatact tactattaaa tcctgttgta agattgtatt
      421 aaaaaattaa tatttaccaa atcgtcaagc ttagaggtgt gacgatttgg tatacttcat
      481 ttataagtat ttgatataat ttaaaattat agattcttag gtttgattta gtcataaaat
      541 taatataatt aactcataag gaggtgtata agattgacaa attttaattt aaaaatacta
      601 tttttaggaa actacattct atcacttctc ttcatgggaa tatctacaag cactgaaact
      661 tatatgaata tccaaagcat tattccctta ataatttcaa cattattact catagacatc
      721 acatatataa cctcttgtaa tgtccgaaaa aataacatta tttcattgtt ttgtggatta
      781 ctagcactag acagttggta tttactctta tcatttgtaa ccacaccaat aggcactatt
      841 gtttttaaaa tattaagcac agttatagtg tatgtctcta ttaaatttat atttttattt
      901 ttgtttcaag gtagtggata caatttccgt aagataacag acattttatt gcttatcacc
      961 tgcattggtg caattgtagg agcatttatt tctaacagaa cgtatgcttg tatgtacgga
     1021 atacagtttg ttgttagtat aataagcttt ttctatatta ttttttatca tttgaaaagg
     1081 gttgtttttg tcataaaaag cgaaaaaaaa cctatatcga tttcagtatt tataacaatt
     1141 ataggattcc ttacttatta tttttttaca ctaagaatat caaatcatat tggtaatttt
     1201 ggaatctatc taactgtatt gatatttttt atgagtattc atggtattgc acttaaagaa
     1261 aatgatagtt ttcctatttc aacagttttt aacataaaac aaagggctat atttcttttt
     1321 ataagtattt tagtaattag cttggtcgta ctatttgcca atgggaattt ttctataata
     1381 ttaatagcta taaatgtttt atttatgctt attttcctct gtaacattat tttggagttt
     1441 aaccttagag atgaaaaaaa taaagtagtt aaaaatagca agtatacttt tgcattagaa
     1501 aaattacaac aagaagaaca actaaaaaca gagttttcta attttttaca tgatgacata
     1561 ttacagaacc tgctttcaat aaaaaacatg atgagtaagt catatcgccc agaagttcaa
     1621 gatattatat atgaaacact taacaatcta aatacatgta taaggaagca aatgcaagac
     1681 tatcatcctg ttattttaaa aaatttaaca gtaaaagaaa attatcagaa tttaattgaa
     1741 agtgtttctt catcctttcc acaaaaaaac atttgtgttt catttaagtg ttctgatact
     1801 ttattcttag ttgaaccata tgatatattg atatatcgac tgattaaaga attattgaca
     1861 aatgtatata aacattcaaa tggtagccat gcatggatag ctttagcact agaaaaaaat
     1921 ataataaaat taaatattaa tgataatggt gtgaaaaata catcttttaa aacgccttta
     1981 gatataacta aaattacttt tgatacaaca aaacacaaag ggattatctc aattaaagaa
     2041 caagtagaaa atctaggagg agcgataaat atttctgata atatacctca tggagtttgc
     2101 gtacagatta caattcctat gaaaggagat ggttcttata aacattttgt tagttgatga
     2161 ccatatgctt tttgctaaga gcttgtcaat tgcactagaa gaatacaaag aaatcgaaca
     2221 gttttattcc acacaggaca tccataatct ttcagaaatg attcaagata aaaaaataga
     2281 cattgtctta atggacataa accttagagg cttaagtgaa tgtgatggct taaaagtagc
     2341 ttataattta ttagagacta attcaactat taaaataata atcttgactg gatacgacct
     2401 cccagtttat aaacatgagg ctaaaaaaat aggtatctat ggattcctca ataagaatat
     2461 tagccctgat agtttaattg attcattatt aaaagtctat caagggggaa cttgttttcc
     2521 atctgaaaat actgaagtaa ttattgaaga tttaactagt atggagaaaa agatattaca
     2581 acttatctgt agtggtaaaa aaagaaaaat gattgctgat gaattgtata taagtgaaag
     2641 aacattatca aatcatttac aacacatata tgacaagcta gatgtttcat catcagttga
     2701 ggctattaca aaagctatta aaatgggtta tatttctcca gtatattaaa aaaagtgagt
     2761 gtctcaaaat gaactttcta gctcataagg cactctcctt tgtattaaat aaaatatatt
     2821 ttcatcattt aaacaattaa gaagaggctt ctctctattt tgagacaact ccattttaat
     2881 acactattta tatttataca taaacatatt ttaaacaact aaaaagcaac taatatctca
     2941 atacatatct tcataacaca tatcaccata attacaagca gataaagaac tctcctcatt
     3001 taaatacgac tcaaattcat acccaaacag aattgttggt tctatatata tttccatatc
     3061 tgtatatctc catcttctta cctttgcctc tataaccttg taaaaatctt ctaaactata
     3121 cccttctttt aatcttttat ctataagtgc cttagttata ctcaaatcag ctttaaaatt
     3181 ttttcctgat tcactattta attttcttat aataatttca tgtatgcttt ctggattttt
     3241 ataagtagaa tcttcagtca aatcttcatc cgaattttca aaggtatttt tatttaaatt
     3301 ctttttattt aaattatttt ttaataattc ttttttcttt gtctcatttt ctgtttcata
     3361 cccagtatga attatcaaat cataatcttt gtctatactc tcttgattta tacggctgta
     3421 tgtgttggta ttactgttct taaacaactc atttctatgt ttatatttca ttgttttttg
     3481 gttattgtta atagttgcat caatgtattt ctctatattt ttgaaaatat caccattctt
     3541 tgttacaatt tctgcattac tactttcaga acaaacataa ctatatacag aacaacaatt
     3601 tccctttact cccctactta ttaatctcaa tatacctaaa tctataaatt catttacaag
     3661 tctacttatt gtagatatag atacatcaag gtcagatgca gctttcctta ctgtcatcat
     3721 aaattgtcct cttggcagag ttccttttaa tctttgtata ttttcttttc tcattacata
     3781 gtgattaaac cttattctat ttacatctct tttttcatta aaatataata ctgctgaatt
     3841 taattgataa aaactattac tcatattccc actcctccaa tttaaataga taatttgttg
     3901 taaagttttt agatttctaa ataacttatt aaatgattat ttttattaaa aagtattctt
     3961 tgatattttc attttaaagt tttaagtata ataagttaat agtgtagatt taactt
//
LOCUS       DAEMHW010000269         4009 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEMHW010000269 DAEMHW010000000
VERSION     DAEMHW010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.271"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(540..1091)
                     /locus_tag="KPK67_003563"
     CDS             complement(540..1091)
                     /locus_tag="KPK67_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2460479.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVILSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1282..2244
                     /locus_tag="KPK67_003564"
     CDS             1282..2244
                     /locus_tag="KPK67_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBG2460480.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            complement(2671..3864)
                     /locus_tag="KPK67_003565"
     CDS             complement(2671..3864)
                     /locus_tag="KPK67_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG2460481.1"
                     /translation="MSEKRRDNKNRILRTGESQRKDGRYAYKYIDTFGKPQFVYSWKL
                     VPTDKTPAGKRDDIALREKEKEIQKDLDDGIDHIGKKMTVCQLYAKQIRHRANVRHGT
                     KQGRKQLMRILQEDKLGACRIENVKLSDAKEWALRMKEKGYGFKTINNHKRSLKAAFY
                     TAIQDDCIRKNPFDFQLNTVLEDDTEPKEPLSHTQEAAFLSFVQHDKVYQKYYDEIII
                     LLGTGLRISELCGLTEADIDLDKQLIHVDHQLLKIADVGYYVETPKTKSGNRVIPMSE
                     KVLEAFQRVLNKRKYAQPVILEGYTKFLFLNRNGLPKVAVNYESMFRGLVRKYNKTQK
                     VALPKVMTPHTLRHTFCTTLANAGMNPKALQYIMGHSNINMTLNYYAHTTSETSITEM
                     KRLIA"
BASE COUNT         1299 a          607 c          723 g         1380 t
ORIGIN      
        1 aattatattt agatatagga gtcttatgat aaaagtaagc ttatgtgtta tttgtagtaa
       61 atataaagtg catatagtgg taaaacaaaa gtttcatata aatgaagaaa aaaatataaa
      121 ataatttatt taactactta aaaataaact aaaagggata ataaaatagc ttttaggtta
      181 tggtaataaa ggtatttaaa aagagtgcat aagcactcca attgtgagaa atacatacac
      241 aaatcattat tacaacaatg aaaaatgtac atataagaga cgttttgtat ttgttttaag
      301 tgataatatt ttaatactaa ctaaaaaatt atcacaatag taaaaaagaa aaaggagtat
      361 tttgtactcc atttgtcaaa aatataaaac ttttatataa gcttattata acataattga
      421 taaaagtgta aagatatata atgaatttat aaagaaaaat aaaataaaaa agataaatcc
      481 taatcaaagg atttatcttt ttgtatagaa aataattatt ctattattaa atggcatggc
      541 taaactggat aataatttat tattactgct ttcaatgttt tttctccttg attatggtat
      601 gtatgaattg attcagctga aaaacaaata gagtcatttt ctctaagttg ataaatagag
      661 tcatttactt ctaattttaa ttgaccttct ataatcataa tatattcctg tgatttttca
      721 gaatgaccaa cagatgtata actatgacct tcttctaatt ccatttgaaa taattcgaag
      781 tttctagttg gtgtattggg ataataacaa taaagacgat aatctttatt ttctgaaact
      841 tgtacatcta tatcagtttt tgatacaata aaagtttcat tttgaggttg ttccagtatt
      901 gcagtatatg gaacatttag tccacttgca attttccaaa ttgtgtttac tgttgggttt
      961 gagtctccct tttcaatttg cgaaagtatt actttactta cgccagataa ttcagcaagt
     1021 tgacctagac ttaagttacg ttcattacgt aatctattta aatttttagc aataacagta
     1081 ttaatactca tagtagctcc ttaaaatgta aattattgac aaatataatt tacgaatgta
     1141 taatttattt aacattattt ataatttaca aatgtataat ataatgaacg ttaaatacac
     1201 tgtacatccg taatataaat tatactgcat aaagagttgt ttttcaacgg atgatagttg
     1261 agagattaag ggggaattgt aatgcgtgta cttaataatg ttacagaggc tataggcaat
     1321 actcatatgt tacatttatc caaaatagca gatgaatttg gagttcaagg taatatttat
     1381 gcaaagatgg aatacttaaa tccgggattt agtaaaaagg accgtgtagc attacaaatg
     1441 attaccgaag cagaaaatgc aggtttatta gttccaggtc aagcagttgt ggaattaaca
     1501 agtggtaata ctggaacagg actagcaata gcttgtgcat gtaaaggata taaatttata
     1561 gcgtgtatgt caaaaggaaa ttctatggaa agagcaagaa tgatgaaagc tttaggtgct
     1621 gaagtagttc ttgtagacca aatgcctaat tcagttcatg gacaggtatc tggtgaagat
     1681 ttggcacttg tagaaattga ggctcaaaaa atagcaaaag aaagaaatgc atttagagca
     1741 gaccaattta atttagaagc ttgtaatcat gcacatgaaa agtatacagc agaagaaatg
     1801 tgggaacaat tggatggaaa tattgatgta tttgtagatt ttcttggaag tggtagtact
     1861 tttgcaggat gttcaaaaac acttaaaaaa cataattctt ctatcaaatg ttatgttgtt
     1921 gaacctgaaa cagcagcaat ttattcaggt aaagaaataa ctgaccaagg gcataaaata
     1981 caaggtggag gatattctat ggatttgcca ttagtagaca agaaacttat tgatggttat
     2041 attcaagtta cagacgagga ggcaactgat gtggcaagaa aattagctaa attagaaggt
     2101 gtatttgcag gattttcttc aggggcaaat gtatgtgcag caattaagtt attaaagagc
     2161 agtgaaaaag gaaaaaatat agttttaact cttaatgata gtggtttaaa atatttaagt
     2221 actgatttat atgaagaatt gtaataatag cacaatagtt atgcaatgtt ttttaaatta
     2281 agtatataga tttaaattat aaaaagtatc tcaatgtaat ttttgaaagt tataaaggag
     2341 atactttttt attttgtaaa tatggagtat cctacttata agtattaagc tatatagata
     2401 ttgatgatta gctatgcaat aaaaaagagt atgtgttttt gcatttatac atcataactt
     2461 ggaataatac atattaatgt tctaaatgtg gaataaatca caaagtaatt tttgattatc
     2521 ttttcaaaag ttcttatatg ccggtatagc ctattgttac aggcttttcc ggcatttttc
     2581 attgtggaag atactctcaa atcttcttat atccccgtat gtttttgtgt tcaaaagtag
     2641 tatgtggagt agtaaattct ccatttacta ctatgcaatc agccgcttca tttctgttat
     2701 tgacgtttcg gaagttgtgt gtgcgtaata gttcagcgtc atattgatgt tggaatgtcc
     2761 catgatgtac tgcaatgctt ttgggttcat tcccgcattt gctaacgtgg tgcagaatgt
     2821 gtggcgtaag gtgtgcggtg tcattacctt tggcaatgcg accttttgcg ttttgttgta
     2881 tttccttaca agcccccgga acatactttc atagttgact gctacttttg gcaaaccgtt
     2941 ccggttaagg aacagaaact ttgtgtagcc ctccaaaata acaggctgtg catatttccg
     3001 tttatttagc acccgttgaa atgcttccaa cactttttca ctcataggaa taacccggtt
     3061 gccgcttttg gtcttaggcg tttccacata gtagcccaca tctgcaatct tcaaaagttg
     3121 gtggtctaca tggataagct gtttgtctaa gtcaatgtca gcttccgtca gtccgcagag
     3181 ttccgaaatg cgaagccctg tccctaacag tatgataatc tcgtcgtagt atttctgata
     3241 gactttatca tgctgcacaa aagacagaaa agccgcttcc tgcgtatggg atagaggttc
     3301 ctttggttcc gtatcatctt caaggactgt gttcaactgg aagtcaaacg gatttttacg
     3361 aatacaatcg tcctgtatgg ctgtgtaaaa ggcagccttt aaggaacgct tgtggttgtt
     3421 gatagtcttg aagccgtaac ctttttcttt catgcgtaac gcccattcct ttgcgtcgga
     3481 gagcttcaca ttttcaatcc ggcaggctcc aagtttatcc tcctgcaaaa tccgcatgag
     3541 ctgttttcgc ccctgctttg taccatgccg cacatttgcc cggtggcgta tctgctttgc
     3601 gtaaagttgg cagaccgtca ttttctttcc gatatggtcg ataccgtcgt caaggtcttt
     3661 ttggatttct ttttcttttt ccctcaacgc tatatcgtcg cgctttcctg ccggggtttt
     3721 gtccgtaggc actaacttcc aagaataaac aaactgcggt tttccaaagg tatctatata
     3781 tttgtaggca tatcttccgt cttttctctg gctctctccg gtgcggagaa tacgattttt
     3841 attatcccgt cttttttccg acatcgttat gctcctttcc ataatggaaa gagccttgat
     3901 atgcttgact acattgtacc acattcaagg ctcgatttca ctattagatt gcgtccaccg
     3961 tatcaatcat tttttcaaac tgcttacgct taatctgaat acggtttcc
//
LOCUS       DAEMHW010000270         3973 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEMHW010000270 DAEMHW010000000
VERSION     DAEMHW010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.272"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(128..415)
                     /locus_tag="KPK67_003566"
     CDS             complement(128..415)
                     /locus_tag="KPK67_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460482.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(435..710)
                     /locus_tag="KPK67_003567"
                     /pseudo
     CDS             complement(435..710)
                     /locus_tag="KPK67_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1347..1526)
                     /locus_tag="KPK67_003568"
     CDS             complement(1347..1526)
                     /locus_tag="KPK67_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460483.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(1811..3304)
                     /locus_tag="KPK67_003569"
     CDS             complement(1811..3304)
                     /locus_tag="KPK67_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG2460484.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLI
                     EFKKLLTYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFE
                     GNIKIGDCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDD
                     YINTEEGAKRLSLLLYAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEEC
                     KVYPDFVLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCF
                     EILRYLKKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRVKLDIL"
BASE COUNT         1408 a          627 c          385 g         1553 t
ORIGIN      
        1 tactggttta aataggttat ctttatttgc ttaaattagt tcaactatac tggtttaaat
       61 aggtttcctt tagtttaaat aattcaccta tacctacttg aatagttcac ttttatttgc
      121 ttaaattcta taccaatact ggcttaaaca ttatactacc atgattgtat tgtctatctt
      181 caactacaat aaaaacaggt tctaaatctt tagtagatgt tatttttaat tctaaaaaaa
      241 cattatcagt tggtaatatt tttacatcat aattatttaa attattattc gtgatactat
      301 taaatacatc accttgacgt tcaaaaaaaa cattatcaac tattacaaaa taaacatctt
      361 cttttctact attttcaacc tctatagtat tttgtttagt tacatttagt ctcatcttcc
      421 acctcctatt aatattactt aattatatta atacttgtaa ttgttgcatt atgttcatca
      481 caaggaagag gtttaaaaac aactatctct gctaaataat tctgtggagg atttgtaact
      541 gaagtagcat tgcaaataaa tttaaaattt gttcctgcta caacttgagt agaaacaata
      601 agtggttcat aaccaacccc ttctaacatt cctacagctt cattaaatac ttttaaatct
      661 tcttcttcga cagctctgaa ttttgactat ccaccaacaa aaagactcat ataaaaacct
      721 cctttatata tatatttatt tatatatata gtagtaccaa ttttctcaat attcaatatt
      781 ttctgtgtaa ctggtcaatg aaaatgcata actggataaa aatacttata aaataactta
      841 actttacata taaatagctt actttacatg taataagatt attctacaat atgaaataca
      901 tcttattttg aatatattct aaatacaata atggatattt tcaattatta taattagaaa
      961 atatccatta cttaatttgt tttatttaaa atcacataga gactttatta ttttttttat
     1021 tgcatgaata gctatttcat gtatcataag gtgatatacc ccctccttga aaccacaaaa
     1081 aacataacaa actttacgaa tcttgtctta aaagaccaca ggtacattcc ttatttgtgg
     1141 attggagtag ccataacaag caaattttac aatttttatg atgacaaaaa tcaataaaat
     1201 cgcttagtat ataacacgaa acactacttg ttactattaa gaatagttag ataggaagta
     1261 tgcattatac caataaagga atcaataaag aaagttaaaa catccctaca aattaaaaat
     1321 ctgttacatt tttctatatt taaacgttat ttacttacaa ttggagaaat aacaggtttt
     1381 ccttctcggt caagtaaagg cgtaaatccg gctgcattgc cataatagtg gaatacatag
     1441 ttcacacctg tttctttatc cacccaaatc tctgttgcat ttatttttcc ttgtgaatat
     1501 actttttcaa atctatcttt tcccatttta taatcctcca tatttttgat tagtctatac
     1561 cttcttcact taagtcgaaa aaatacataa cttatttttc taattctaat atgaacacgt
     1621 aaaaatgtca aacagcttat ttaatttcat aacttcatgt tactgttcgt aaccttgtct
     1681 gttcacttac actacatcac agcaccattt taccctttac atgattgtat tgcacacagc
     1741 aaaaatgctt actttattgg atttttcaac taaaaaaagg attttttctc cacagaacat
     1801 ttactatttt ttataatata tctaatttta ctctcatatc aacatttaat gaagatataa
     1861 cctttttcaa atattgtaaa gactccttaa aaatattaga aaaagattct gcaaatctct
     1921 caacatctcc tttttttaaa tacctaagta tctcaaaaca tacatcaaaa cgtgttttca
     1981 aaatttcatt atattcttca tgaaaaaatc tctctaaata gattccaaat atcatatatt
     2041 gttttaaatg caaaaatgtt tcctgtaata cataaatttg tgtataatct actatcttac
     2101 aaagtaaaac aaaatctgga taaaccttac actcttcaca attattttgt aagtacctta
     2161 aatgagtttc tattttatct acagctatca aatccatttt atttcctagt aaaagtacta
     2221 catcctcaag agtaatcgta acaaactcca tagcatacaa aagcaaactc aaccttttag
     2281 ctccttcttc tgtatttata taatcatcat catattcaga aaaaagtgtt acatatgttc
     2341 ccttgccatt aattgtttta gcaattccca attcatttag ctcattatag gcacttctaa
     2401 ctgttggaag agatacatta tatctattaa ttatcgaagt aattgatggt agacaatctc
     2461 ctattttaat attgccttca aaaatttgac aaattacatc tgaaacaatt aagtcatata
     2521 aataacattt ttccaacgtt acagaattaa aactacttgt atctatactt tgtggagcac
     2581 gattttctac cattttaagt atggattctc tacaagcttt atacatatat gttaatagtt
     2641 ttttaaattc aattagattc ccgtccaatc catataagta agtttttctt acctgctcta
     2701 tgtatttatt acgaaaaaag tctatacaac aattatccaa gttatccaag ttatccaagt
     2761 tatccaagtt atcgaaatta taggccaatt gtgaaataat gacatcatgc tgaataatct
     2821 cacaaaaatg gtgaatcagg aaattatttg ttttacttat tagtttattt atatatttta
     2881 gcctttgttc tttaaagact aaataattag aactattttt ttccatacaa tcgataatct
     2941 cgtttaattc ttcaaagtct ttagatgtaa atcttttaat attgtacata gcaatagaag
     3001 gaaatattaa actaaaaatc ttatatattt ctgatatttg taaaatcttc tcatataata
     3061 agtcagattc tattgattgt ttactcgtac tctggattaa aactggtcta gaaccagctt
     3121 ttgtctctat aaagcccttt tctgagagca tttttattac agtttttata gtattttttg
     3181 ataccatata ttcttctgac atgcttttta tagaatcaag cctatctcca atagtaattt
     3241 taccagtata aatttgttga attatatgct caaaaataat taaatattgt ggttttctct
     3301 tcatattttt cctcttcgat aaattagatt tattgttttt tgtaaggtat atttactttt
     3361 atttaatagg gcaaaatagt tgcttatttt ttcctcataa tctataatta atataaacca
     3421 tttatatata tggtctaagt tttataatac tgtaatgcag taaaatagca tattgtagct
     3481 atactacagc aaattaacat accattgtag tatattaatt ggaataattt taagtacttc
     3541 aaaattccag caaaacatat atgtgttttt gatattatgt atattcgtta atagaatagc
     3601 tatacttgtt ttacataata aattatatga tacttgctat atgtaattac tatatttaat
     3661 acacataact attatactta ctatatgtaa ttactatatt tgatacacat aattattata
     3721 catactatat ataattacta tacttggaca cataactatt atacttgcta tatgtaatta
     3781 ctatacttta tacacatagt tatcatacat gctatatata attactacac ttggacacat
     3841 aactactata tttactatat gtaattatac tatacttgga catatagtta tcatacatac
     3901 tatatgtaat tactatactt ggacacataa ttattatatt tgctatatgt aattatacta
     3961 tacttggaca tat
//
LOCUS       DAEMHW010000271         3899 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEMHW010000271 DAEMHW010000000
VERSION     DAEMHW010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.273"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            348..965
                     /locus_tag="KPK67_003570"
     CDS             348..965
                     /locus_tag="KPK67_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HBG2460485.1"
                     /translation="MKGNEMENYKFVFDLDSTLTKQEILPEISKCINKYELMQNLTNE
                     TMLGNLSFEESFKKRVDLLKCIPISKVKSIVAKINLNEKIVKFIKENPDRCTVVTNNL
                     DIWICDLMKELSLENKYYSSIAHSNGDFIDKIKVIIKKEDIIKKMKGPIVAVGDGSND
                     IKMIENADIGIGFGGVRPIAPGILKVCDYAFYEEERLCQLLKQLL"
     gene            938..1582
                     /locus_tag="KPK67_003571"
     CDS             938..1582
                     /locus_tag="KPK67_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HBG2460486.1"
                     /translation="MPTTKTIVVNCAGRGSRLGLNSNKSLIDIDGKPLIIRQLECLDD
                     YDDIRIVVGFQAEKLIEVVCSYRKDIMFVFNHDYLNTKTAASLSLGKKYSRDTVISLD
                     GDILFFKEDFDNFVNTDGEIIGVCQPYTKDPVYVKTDFINDGEFATEFSRTNGDYEWT
                     GLFKIYSKNLSSGTNHVYKMISPNLPLKIAHVELREIDTPEEYNEALKWYRNKK"
     gene            1595..2335
                     /locus_tag="KPK67_003572"
     CDS             1595..2335
                     /locus_tag="KPK67_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG2460487.1"
                     /translation="MGNATRIYEKHVDIKKDTVQDFWNKRAKKHIPENPYISVKCNDQ
                     NPDYAKNLDKYEKEIIIPKLNITRNSNILDIGCGVGRLADDIVPICNYYLGTDFAEDL
                     ISIAKKRFSDNDCDFQVCDFINTLYDTKVKSKMPFDTILLAGVTMYINDDELKIALKN
                     LLGILDKKATIYISIPIGINKRLTLKEFYSKELNSEYNVIYRTIEEYEEILKPLLDEG
                     FEIVESKSFLTDINQYSDTERHYFILKR"
     gene            2373..3848
                     /locus_tag="KPK67_003573"
     CDS             2373..3848
                     /locus_tag="KPK67_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBG2460488.1"
                     /translation="MNYNKKNEVLNLIQILIEGIDYIKNNNLSESIVNSSLEAINYMK
                     KYINNSGRSNKNLLNLIDETYVKILRLSMYKNIRLIEDCELIISNLNYLSNIIENSLN
                     KETKIVFMPYNAKMWNSLESIWKSAVLDEQCDCYVVPIPYYKLIDTPNGITQIYTYEG
                     NDFPEDVPVIHYDDFDLSKEKPDIIYVHNQYDDCNNATMVDSNYFSYNLKQYTNMLVY
                     VAYGILGTYPVSFYLNFYELIASRNFDKVIVQSPAFEIIAECSGINKNQVLTMGSPKF
                     DSLIYNLKQKNINKNYESKLKGKIIFLWTTNLMKIPNGKDGVIDEIENVFDIIENSQE
                     YGLIYRPHPLELEYVKSKVPECFNRYKTLLDSISIKNNIILDDSVSYYESFNLSHALI
                     TDRSSVLIEYIQTKKPILIYDIDMERGYYDSRIFDIFSNYVVGEEDMDLIKFMNLVKN
                     NNDYKLNQRLNSLNSVLSNTDGSCGEKIHTNVLEYVLNNHI"
BASE COUNT         1578 a          372 c          596 g         1353 t
ORIGIN      
        1 acgaaggttt tgcacattaa gacaattttt tagtaatttc aatgatttta gtgtttttaa
       61 ataattgaat taattcgaca taaaacagca gtatttgtaa taattgaatt tttattaagt
      121 cattttaata cttaaaaacc atttatagag tatttttgtt agttaagaac acaagatttc
      181 tttattttga taagatttag tttttagatt tttgattgag ctatatacaa ttttataata
      241 aagaataaaa ttatattata aaattataaa ctctgtaaaa ttattgaaat ttctaactca
      301 atattgtttg atgtacttat cactcaaaaa taggatttta tgcttcaatg aaagggaatg
      361 aaatggaaaa ttataaattt gtttttgatt tagattcaac attaactaaa caagaaatac
      421 ttcctgagat ttctaagtgt ataaataaat atgaacttat gcaaaatctt acaaatgaaa
      481 ctatgctagg aaatttatct tttgaggaaa gttttaagaa aagagtcgat ttattaaaat
      541 gtattcctat ttctaaagtt aaaagtatag ttgctaagat taatcttaat gaaaaaattg
      601 taaagtttat aaaagaaaat ccagatagat gtactgttgt gacaaataat ttagatattt
      661 ggatttgtga tttaatgaaa gagctttctt tagaaaataa atactattca tctatagctc
      721 attcaaatgg tgatttcatt gataaaatta aagttataat aaaaaaagaa gatattataa
      781 aaaaaatgaa aggtcctata gttgcagttg gtgatggcag taatgatata aaaatgatag
      841 agaatgctga tattggaatt ggatttggag gggtaagacc tatagctcct ggaatattaa
      901 aagtatgtga ttatgcattt tatgaggagg aacgtttatg ccaactacta aaacaattgt
      961 tgtaaactgt gcaggtagag gaagtagact aggattaaat tcaaataagt cgttaataga
     1021 tatagatgga aagccattaa taataagaca attagagtgt cttgatgatt atgatgacat
     1081 acgtattgta gtaggctttc aagcagaaaa attaattgaa gttgtatgtt cttatcgtaa
     1141 agatataatg tttgtattta atcatgatta cttaaacact aagactgctg caagcttatc
     1201 tttaggcaaa aaatattcta gagatactgt tatatcacta gatggtgata tacttttttt
     1261 caaagaagat ttcgataatt ttgttaacac agatggagaa attattggtg tatgtcaacc
     1321 ttatactaaa gaccctgtct atgttaaaac agactttatt aatgatggag agtttgctac
     1381 agaattttca aggacaaatg gagattatga gtggacaggt ttgtttaaaa tatattctaa
     1441 aaatttaagc tctggtacaa atcatgtata taaaatgatt tcacccaact taccacttaa
     1501 aatagctcat gttgaattga gggaaattga tacaccggaa gaatataatg aagctttaaa
     1561 atggtatagg aacaaaaaat aaaggagatt ttatatgggt aatgcaacga gaatttatga
     1621 aaaacatgta gatataaaaa aagatactgt tcaagatttt tggaataaac gagctaaaaa
     1681 gcatattcca gagaatccct atatttcagt gaaatgtaat gaccaaaatc ctgattatgc
     1741 taaaaattta gataaatatg aaaaagaaat aataattcct aaactaaata taactagaaa
     1801 ttcaaatata ttggatatag gttgtggggt aggtaggtta gctgatgata tagttcctat
     1861 atgtaactat tatcttggga cagattttgc tgaagactta atatctattg ctaaaaaaag
     1921 attttctgat aatgactgtg attttcaagt atgtgatttt atcaatactc tatacgatac
     1981 aaaagttaaa tccaaaatgc catttgatac aattctattg gcaggtgtaa caatgtatat
     2041 aaatgatgat gaattaaaaa tagcattgaa aaatttatta ggaatattag ataaaaaggc
     2101 aacaatatat atatctatac ctattggaat aaataagaga ttaactttga aagaatttta
     2161 ctcgaaagaa ttaaattcag aatataatgt tatatataga actatagaag aatatgaaga
     2221 aattttaaaa ccattactag atgaaggatt tgaaattgtt gaaagcaaaa gcttcttaac
     2281 agatataaat caatactctg atacagaaag gcattatttt atactaaagc gatagtttta
     2341 atataaataa aatcaaaatt aaggtgaatc tgatgaacta taataaaaaa aatgaagttt
     2401 taaatcttat acaaatactt atagagggta ttgattacat aaaaaataat aatttaagtg
     2461 aatcaatagt taattcatct cttgaagcta ttaattatat gaaaaaatat ataaataatt
     2521 ctggaagaag caataaaaac ttgttgaatt taattgatga aacttatgta aaaattttaa
     2581 gactttccat gtataaaaat ataagactta ttgaagattg tgaacttatc ataagtaatt
     2641 taaactattt atcaaatata atagaaaata gtctaaataa agaaactaaa attgtattta
     2701 tgccttataa tgctaaaatg tggaatagtt tagaaagtat ctggaaatct gcggttttgg
     2761 atgagcagtg tgactgttat gtagttccca taccttatta caagttaatt gatacgccta
     2821 atggtataac tcagatatat acatatgaag gtaatgattt tcctgaagat gttccagtaa
     2881 tacattatga tgattttgat ttatctaagg agaaacctga tataatatat gttcataatc
     2941 aatatgatga ttgtaacaat gctacaatgg tagatagtaa ttatttttct tataacttga
     3001 aacaatatac taatatgctt gtatatgttg cttatggaat acttggaact tatcctgtta
     3061 gtttttactt aaatttttat gaattgatag catctagaaa ttttgataag gttattgtgc
     3121 aatctcctgc atttgaaatt atagcagaat gtagtggtat aaataaaaat caagtactaa
     3181 caatgggatc tccaaaattt gattctttaa tttataattt aaaacagaag aatataaata
     3241 aaaattatga atctaaatta aagggtaaaa taattttctt atggacaact aatctaatga
     3301 aaatacctaa tggcaaagat ggtgtcattg atgagattga aaatgtattt gatattattg
     3361 aaaatagtca agagtatggt cttatatata gaccacatcc attagaactt gagtatgtaa
     3421 aatctaaagt tccagaatgt tttaatagat acaaaacttt gctagattca atatctataa
     3481 aaaataatat tattttagat gactctgtat cttactatga atcattcaac ttatcccatg
     3541 ctttaataac agataggtct tctgtattaa tagaatatat tcaaacgaag aaacctatat
     3601 taatttatga tatagatatg gaacgtggat attatgattc aagaatattc gatatatttt
     3661 caaattatgt agtaggagaa gaagatatgg atttaataaa atttatgaac ttagtaaaaa
     3721 ataataatga ttataagcta aatcaaagat taaattcttt aaatagtgta ctatcaaata
     3781 cagatggaag ttgtggtgaa aaaatacata ctaatgtatt agaatacgtt ttaaataacc
     3841 atatttagtt aaaattaatt aataattaga taaatttagg cttggaaagg agttaaaac
//
LOCUS       DAEMHW010000272         3879 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEMHW010000272 DAEMHW010000000
VERSION     DAEMHW010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.274"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..578
                     /locus_tag="KPK67_003574"
     CDS             <1..578
                     /locus_tag="KPK67_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBG2460489.1"
                     /translation="IISVTMLILISHINILIIADIKNKDICLKLNIKYMFNLININRQ
                     LYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHELYSNINFGTGNIGL
                     TSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHIRPRIKALFNIIIM
                     INKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            532..960
                     /gene="ytfJ"
                     /locus_tag="KPK67_003575"
     CDS             532..960
                     /gene="ytfJ"
                     /locus_tag="KPK67_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBG2460490.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1208..1648
                     /locus_tag="KPK67_003576"
     CDS             1208..1648
                     /locus_tag="KPK67_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2460491.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1664..3115
                     /locus_tag="KPK67_003577"
     CDS             1664..3115
                     /locus_tag="KPK67_003577"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBG2460492.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVDGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAIETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3206..3874)
                     /locus_tag="KPK67_003578"
     CDS             complement(3206..3874)
                     /locus_tag="KPK67_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBG2460493.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT         1622 a          354 c          669 g         1234 t
ORIGIN      
        1 ttattataag tgtaactatg ttgattttaa tatctcatat aaacatctta ataatagctg
       61 atataaaaaa caaagatatt tgtttaaagc ttaatataaa atacatgttt aatttaataa
      121 atataaatag acaactttat ccagctgaaa atagtaaaaa taatgataaa aaagagggga
      181 tgaagaataa tatagattca agtatattat tagctgatga ccttttgagc atttatagat
      241 tattaaaaaa gataaaaata catgaacttt attcaaatat taattttgga actggaaata
      301 ttggcttaac atcttctgta tatgtattga taaatacttt gtatggaaat ttatttaata
      361 tgattgatgc agaaaaaatg tatttaaatg taaatccaga ttttacaaaa gattatgttt
      421 taggaaatat aaggatacat ataaggccaa gaataaaagc tttatttaat attataataa
      481 tgattaataa aattatgaat aaaaataaag gaaataagga aggtgatagt aatgagagca
      541 acaggttcga tacagagtct tatggaaaca actcttgaaa caattaaagg ttctatagat
      601 gcaaatacta ttataggaga ccctataaaa acagatacta cagtggttgt acctatatca
      661 aaggttacaa ttggctttgg tataggagga ggagaatact caaaaggata tgatgataaa
      721 gatagggaaa ttgaattaaa aaatgaaaaa tcagatacga attttgctgg aggtagcgca
      781 ggggctatat ctgtacaacc agtagcattt gtagtagttg aaagtggaga gactagaata
      841 atgagtttag acagtaatat caatctagtg gataatatat tatctataac tcctagagta
      901 ttggagaaaa ttcaaaatat atcacaaagt aataagaatc aagataaaaa caatatgtaa
      961 gaataaaata tttttaaata tgtatatagt tttaggtaaa gtagtggggt tagtataaat
     1021 cccactattt tgttgtgtac tttaaacatt tatatctaat aagattttag tttaaaacaa
     1081 tttaaataga taataaagtt gatatatagt ataattataa aaagaaaaga agtattatat
     1141 tatagatata taaacgcatt tgagatataa gagggagtta taaatcatta gttttgaggt
     1201 gaatgttatg agcgataaag aattatctat tttgttgtta gaaagtatga ataattttca
     1261 taatttaata aagattatta ataacgaaag atataaaaaa gataaggttc ttacagaaag
     1321 acagttcttt gccttagtaa aaataagaaa acatgataaa atagagctta aaaatctaag
     1381 tagagattta catgtatcta cttctagtct gtgtatacta cttaataaat tggttgagca
     1441 agagtatgta tatagggaag aagatagtag agatagacga aatacttttt atggaataac
     1501 taaaaatgga gaaaagatat tggataatga aatactaaag tttgtatcta taataagtga
     1561 taaaatggat tgcttagata tagataataa agataaatta tttacttctc tggaagagtc
     1621 taaaaatata atagaacaat tattttaaaa aggagaattt attatgaata taaagtattc
     1681 taaagaaata ttaaaaagtt tagaaaatgc tataacagta gtagctctat ttgagggaga
     1741 agatgtttct acgacatttg ataaaataaa tatgataagc agaaatttta attctaaaga
     1801 aaatagtata gatactgtaa cattggttga aaataacaat ttggtaaatg tttgtcttgt
     1861 tggttttggt aagagagagt tattgaatag agaaaaaatt agaatcatag gtgggaattt
     1921 gtgtaaaaaa ttaaaaaata tattgaaaaa tgaagcatat aagagtagtg atgttaatgt
     1981 actgaatctt aatttaaata gtgatgaaat aggttgtttt gtagatggga tattacttgg
     2041 aaattataag tttgataagt ataagacaaa aagtaaggat tttgaaagaa aagaagttga
     2101 tactataact atatttactg aaaaagaagt tgagtgtcaa atagaaaagg ctaaaatatt
     2161 agctaattca actataatag ctagaaatct agttaatgaa ccatcaaacg taatatatcc
     2221 aaaaacatta gcaatagaaa cagttaaact tggcgcagag tttgggttta atgttgatgt
     2281 ttatgaagaa gaaaaaataa aggcattggg aatggatgct tttttcgccg tttcaagagg
     2341 ttcaattaat aaaccaagat tcatagtaat gagatacttt ggagataaag aaagtacaga
     2401 tatattaggt ctagtaggta aaggattgac ttatgatact ggtggatatt ctttaaaatc
     2461 aaatgcttct atgctagata tgaagacaga tatggctggt gctgcgagtg ttataggtgc
     2521 tatgtgtgct atatctcaaa gtaaattaaa gaaaaatgtt atagcagtag tggctgcatg
     2581 tgagaatgct ttatcaggag gttcatacaa acctggagat ataattagtt ctatggcaaa
     2641 gaaaactatt gaagttttaa atacggatgc agaaggtaga cttacattag ctgatgcaat
     2701 atactacatt ataaacaatg aaaaagtaac aaaggtggtt gatgtagcta cattaactgg
     2761 tgctgcttta acattgttag gtaatgtcgc aactcctata gtaacaaata atgatgattt
     2821 ttattgtgaa ttagaaaaag ctgccacttt atcaggggaa agggtatgga agatgccaat
     2881 ttatgatgaa tttaaagata tgataaaagg agaagaagca gaccttaaaa acactggtgg
     2941 taaaaatgct ggatgtataa cagcaggagc ttttattggt gaatttgttg ggaatactcc
     3001 ttggatacat atggatatag cggggacaag tacatcatca aaatcaattg gatacaaagc
     3061 aaagggggct acaggagaac ctgttagaac attgtactat ttggcagaaa tctaaaaaac
     3121 aattataaaa tataatttat aaagccacgt ggatattttt gtaatttaag attatctatg
     3181 tggctttatt ttttatttaa ataaattatt tactcttttt ctttttttca agtataaaag
     3241 tatctctatt aaataaaaga gcaggtatgt acataatact agtaacaaaa ataaacgtaa
     3301 aaatagcagt tgtgctatga cttaaaaaac cataatagta cctagagttg gtaaatgtgt
     3361 ttagtaaaaa tatgaatgaa aaataaataa aacatatagc taagttatct ctaagtgcat
     3421 atctgttaac tctaaatttt ctaacatcaa ccatatatat atctgcataa attatcaagc
     3481 acttagagaa gataaagata aaatatatta agctaggaaa ttcatagccc aagtttttag
     3541 aaaaaatgag ttctccaact gaacaaacta aactccaaga aaaaaatata ttaaaaagat
     3601 gatactgctt aaaaattagt atggctatac acagatatat acaaatataa ttgattccaa
     3661 ttggtaaaga gtttagagta ctataattac ctgaagaaat aagtaataat tgttctagag
     3721 ccataattat tacaagtaat gtacaaatta tcttctcaac tgtatagcta taaggtaaaa
     3781 tatttttggt tagttttgga caatagtata aaaataatcc aaatataaag agacttacaa
     3841 attgttcact agaaaaaaga aaaattttaa acatataac
//
LOCUS       DAEMHW010000273         3852 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEMHW010000273 DAEMHW010000000
VERSION     DAEMHW010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.275"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(60..878)
                     /locus_tag="KPK67_003579"
     CDS             complement(60..878)
                     /locus_tag="KPK67_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG2460494.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            1046..1627
                     /locus_tag="KPK67_003580"
     CDS             1046..1627
                     /locus_tag="KPK67_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG2460495.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            1624..1818
                     /locus_tag="KPK67_003581"
     CDS             1624..1818
                     /locus_tag="KPK67_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460496.1"
                     /translation="MKIITIGSSLITVLLFLSTMICGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(1982..3724)
                     /locus_tag="KPK67_003582"
     CDS             complement(1982..3724)
                     /locus_tag="KPK67_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBG2460497.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTTKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFNDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
BASE COUNT         1364 a          519 c          504 g         1465 t
ORIGIN      
        1 acataacact aaaagtaatt tgtctaacaa attaaaatat tatccaatta atataaaaat
       61 tacttctttt taactgggaa gcatacctct gttacccaat tatcaggatt actatcgttg
      121 ccaggactta catgataaat attaaacata ggaccatcca catcatagtt attatctgat
      181 atccaattac caatagcctc acaagcgtct gcaacttgat tgaaatttcc attaacaatt
      241 gcagttgcag cagtcacact tggaacagtc ttaaatttaa catgttcagt atctttatac
      301 tttccagata cagcaacttg tacttcaaca tctacataat cttctttata tccagtatca
      361 taaaaaactg ctttagaata ctttgggggt tcaatttgaa cattctggtc ttttgtctct
      421 aaaaatgctt gatgccataa cattccttcg tcttgataag ttggtataac ccttctaagt
      481 gtcatcatat attttggtgc aaaattcttt atagttacat cataattttt cataatatca
      541 tttcctccaa ttcttttaat agtggtttct atttttaata gttttttctg tgtatcttct
      601 aattgttctt ttacttgaga atggtggata tttagatatt ttatgagact ttctttatcg
      661 ttatattctg tcaaaatttc ttttatagaa tacaagccaa agcccatatc ttttagtgca
      721 tggattctgt ttgtagtaga aagttggtct gcactatagt atctatatcc attagtttta
      781 ttcgtatgag aaggagttaa aagtcctatt tcatcataat gacgtaacat acgaatacta
      841 attttactta gctttgaaaa atcgcctatc ttaaacatgt ttttgtctcc tttataaagg
      901 tacttttagc ttaatattaa tataaaccat atcataatgt tagagtcaac taaaattata
      961 attaaattat attgacatag gatgaaaaaa atattatact gaacatgttc agaaccgaaa
     1021 atgttaattg ttttggaggt ctattatgaa aagtaatgat aaagatgcta gagaacgtat
     1081 aattgaagtt acattgaatc ttttaaatga agtagatgat attgaggaga ttacagttag
     1141 aaaaattgct gagcgtgcaa atgttggagt tggtttaata aattatcatt ttaaaacaaa
     1201 ggataatttg ttgagtactg ccataggaga tgtgatgtca aatataatag cagagctata
     1261 tgacgatagt gtatatactc taagacctat tgaagatttg aaaaatttat taaaaaaatt
     1321 gtgtgatact ggattacatt atgaaaaagt gcttcctttt gtattgaatc aatgtataac
     1381 caatggagat atgcaagcag aacttgatat tgtccctatg cttcgtaaaa tttttggaaa
     1441 taaaaaagat gaaatgagtc taagaatcat tgccctgcaa attattttac caatacagat
     1501 ttctgcttta tctacagaat catttcagtt atacagtggt ataaatataa agaataaata
     1561 tgagcgagat aaattcattg atattttaat agaaaatatt attggagagg atgttgatgt
     1621 aagatgaaaa ttataactat tggttcaagt cttatcactg ttttactttt tctttcaact
     1681 atgatttgtg ggttctggat taagaataat aaggtaacag atgcaagttc tattaagttc
     1741 catatgaata gtgctatttt tacagggata tttttattga tttccacaat ccttttaatt
     1801 atatatataa aaaaataata atattattaa taaataaagt atcagaaatt ataaagacaa
     1861 gagtcttaaa aatttctgat actttaccat ataattttat taaacattaa ttgtgtgtga
     1921 aaaaatatca cttatttcaa tctaatacta aatcttaaga atgagtataa agtattagaa
     1981 attaagaagc attttttagt tcaagtggat tatctatacc aaaataatca tataaagtag
     2041 agcttatttc ctctaaattt ccatcttgga aaggtaattg taaatttacc ttttctacca
     2101 catcattaaa tccagcatca gtattttgat ttaaaaaatc catatataca tcaacagctt
     2161 ttctgtaatc ttcttgtgat aattgccata tctgaagtga tacatcagat gcaagaccat
     2221 aactcaagta ataaaaagga acttggaagt tatgagatac aagtatccaa aaaggagcat
     2281 ttttttctct aagtatatta ttggaaactc catattcttc ctctaaatca aaaaacaatt
     2341 tattgatatc atttagactc atatatggat tcttgtatat aatttcttga aattcatcat
     2401 aaagacaagc atcaataata gtatttaaaa caatcgataa gtgttctttt tttatagctt
     2461 cagattgttt accaaaaaaa tcatcaaaat atttataaaa tagaatttct aaagatgttg
     2521 aatgaacttc acatacatct atacttggtt gcatcttatt atttaagtta atactgttgt
     2581 agtaattata aaaatgacca aactcatgag caaaactagt aacacctaaa aaagaattgt
     2641 cccatgtatt aaacaagaaa ggtgctttat atttatttat ataagtagta tatcctccag
     2701 gtgatttgtt ttttccagaa gaataatcgt acaagtcata ttttttcata tagtcgaagc
     2761 tctcttttag ttttggagat atatcttcaa gtactttatc aaacttccta aatgagcgat
     2821 ttttatatac tttaatataa atagaagaat cagatggttt agatgaaatc tctctatata
     2881 atggaactat gtaatctttt acatcttgtc taaatttttt agcttcttct tgagaatagt
     2941 ctttgtttag gttcatatag gcataatcta tataattctt aaatccttgc ttacttgcta
     3001 tttcagttct tgtctgtata agttctaaga atatgttacc aaatatagga ttgtattttt
     3061 ttaagtaatc tgaataaatt ttcacatatt cttcagggct aagatttggt cttgacaagg
     3121 cttcttcaaa gtctacctct ataccatcta ttgatactgt agatttagct aataagtctt
     3181 catattttac aactaggtct tgctcttttt taagcaaacc ttcaacttct ttagaattaa
     3241 gttttccaat gttctttaga taattaaatg tatcctttcc aagttctttt tctaattctt
     3301 tagagaattt agaagttaca aatctatctt gaaagtcttt gtatgacatg tcaacatcta
     3361 ctgatttact cataagatac ttatattcct tagagtaaaa ttcgtctgat gaatctatgt
     3421 tatttctaat ttttgctata gtaagtgtac tgtaaaaatc ctgataaatt ttatcaactt
     3481 cattaaataa atttaattgt tccttagttg ttttagctgt taaaagtttg tcgttacact
     3541 tgttaatgtt ttcgcaaatt gactgtatat taggacgctt atattccatt ttttcaaaag
     3601 gaatagttcc tctaacagaa tctttttttg tatcattagt ataatttctt atgtatgatg
     3661 caaatataat aaaaactgca attaaaatta gaaaaacaaa tattttcttc ttggatttaa
     3721 ccataaaaat cctccaatca tggatatagt tattaattat tttactatat taaatataaa
     3781 aaaggcatgt taagaaaatt taacataaag atatagataa gattataaat aatataaaaa
     3841 atttaacttc aa
//
LOCUS       DAEMHW010000274         3790 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEMHW010000274 DAEMHW010000000
VERSION     DAEMHW010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.276"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(130..699)
                     /locus_tag="KPK67_003583"
     CDS             complement(130..699)
                     /locus_tag="KPK67_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG2460498.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(771..1379)
                     /locus_tag="KPK67_003584"
     CDS             complement(771..1379)
                     /locus_tag="KPK67_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG2460499.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDNVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            complement(1387..3150)
                     /locus_tag="KPK67_003585"
     CDS             complement(1387..3150)
                     /locus_tag="KPK67_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBG2460500.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(3193..3660)
                     /locus_tag="KPK67_003586"
     CDS             complement(3193..3660)
                     /locus_tag="KPK67_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460501.1"
                     /translation="MKMLNGIIKLFYSEIVKTKIKNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
BASE COUNT         1246 a          667 c          342 g         1535 t
ORIGIN      
        1 acatttttta taatacccct ttattttata aattaatcta atcgattaaa tttatcctat
       61 agaaatttat tacatttaaa aatatgctag caaataaaat atctactagc ataaagtcta
      121 tttataatat taatcaacta ttaattctac taaatctcca tttttaacgc ctgctgcatt
      181 tccctcttcc atatcaacat gcatttctag attaaagctg tcactagctc ttactaatac
      241 attttcaaat actaatgctc ttggtccaga tgttttaact tttactattt gcttatcagt
      301 tacaccaaat ttagcagcat cttcattgct catatgtata tgtctagaag caactataac
      361 tccttctttc atttcaactt ctccagcagg tcctactaat ttaacacctg gagttccagc
      421 tatatcacca gattctttta ttggtgcttt tactcctaat gggaaaccat ctgctaaaga
      481 tatttcaact tgagtatgag ctctagctgg tcctagtact ctaactcctt ttaaagttcc
      541 ttttggtcct actaaatcca ctttttcttc acaggcatat tgccctggtt gagataatgg
      601 tttaaattta gttaactcat gacctgctcc aaataatacg tctatatcag cttgactcaa
      661 atgaacatgt ttattagata atgctattgg taatttcatt gaaattccct ccttttaatt
      721 atttaaatta ttgcaataca attatacata atattaccta caaaatcaaa ttatattgaa
      781 tcaatatata tctttaaatt tttatatatt atatctttaa attcttgatt atctatataa
      841 gatacattat ctgtattgtt taaatatata ttgtgtaaaa gtttagtatt tatactctta
      901 aagaaaaaat ccatcacaat ctgtccacct ttaaactgtg aatcatatga tttactcccc
      961 cccactgcaa tatacattcc tatccgtttc ttacttctat caattgaagg tttatttaat
     1021 acatatttac ttgcatatat agcttgagtt ctatcaacaa cagtctttaa tttagctgat
     1081 attgaatcaa aatgtacagg acttattact atagttcctt ttgaatcatc aaacatttta
     1141 tataatggct tcatatcatc attaaacttg caataccctg ttttttcaca aaatccacat
     1201 gctgtgcaat attcaatatc catattgtag atattaaata ttttacaact taatccacac
     1261 tcttctagca tagcttttac ttcatttgct ataaaactgc aatttttatt aattctggga
     1321 cttccattta ttattataat ctctggactt ttaagtcctt ttttatcgtt tgaatccata
     1381 atcaatctac ctatccttta acataactat taaaatatca tcatcataat tatcttttga
     1441 tgagaagttc tttaaatcca attttagatt ttctactatt aactgttgat ttaatctaaa
     1501 attttctttt agaaagtttt ctaatcggtt tataccatac tcttcttttg atgaattttt
     1561 tatctctaaa attccatcag tgtacataca taccattgca taatcttcca ttgaaagagt
     1621 attattttca taagtagcat cttccattat tccaataggt atgccctttt tacaatttaa
     1681 attattttct attgtgccat ctttctttac aactacagga ctatagtgac ctgcattaga
     1741 tacagtaatc atattttctt tagtatctat aatacctatc aaacaagttg taaatactcc
     1801 catcttgtca aattcatcat ataacatcct atttaaattt gtcattattt ctcttggtgt
     1861 attgtattga ttgcataata ctttaaaagc accttttatc attgcaacaa cataatttga
     1921 taccattcca tgtcccatta catcagcaac aatataaact atatgttcat catccacttt
     1981 tgtagcataa taaaaatctc cacctactac ccttgctggt ttatggtaaa aataaagttc
     2041 tttctcatca ttacaatcca ttttaccttt atccataata agctgttgtt gtttatttaa
     2101 tatgttcaac tcgttttcaa gaactttgtg ttttacttcc ttttcattat gttcatataa
     2161 ttgcatacca acagaaactt gtttggacaa tatactaaga atatttaaat catcactagt
     2221 atagtttttg ctatttgcac aaactataca tcctatagta tcatttttat cctgaagttt
     2281 gtaatatata taactttcca aatttattcc cttttttgag catggtttta aattatttag
     2341 ataccccccc aatgactctt tctttaaaaa catatcaatc ataggatata aatttgtttt
     2401 ctttttttgc tcaacattat aacaactaac tatagcatcc ttattatgat gactatttat
     2461 aattattaaa gcttctttac tattagtaat attacacgct tgtttactta aattttccaa
     2521 aaatccatca atactcttat taaggtatag ttttttagta gcttcattta tggcacttat
     2581 tgagcctctt agtttatcaa ttaaaatttg tacattatta tttttattag ttatttctat
     2641 tgataaactt aagagtgatg caacagatga tataaaatct atatcttgtt ctgttatata
     2701 atcatcctct tttaaaaaac aagtcataaa tccaacaact acgttattta ttataaaagg
     2761 aaaaactatt ctcccaatat aaccttcagc ttttgctaaa tctcgttcac cttctgctct
     2821 agaatcttca aatacatttt ccacatatat tatctttttt tcatttacag cctcatgtat
     2881 atactctgga tatgtgtcaa aatcaatttt tgcaccttta agactattta ttggaaaaat
     2941 ttgaggtaca tattctaagg tttgcgaaca tactaagtag gcatatttag aatcattttt
     3001 ataaaataga ttcacacatg cctttgtagg gtgaacaact tctaacattt tttctattat
     3061 gtcatctttt atattaaaaa aatctgtaga ttcagtcacc atagcggata tttccactaa
     3121 attcttcatt ttataactgc cattagtcat actcaatttc tccccctata tattaaacta
     3181 tttattagta ttttaacata cattctttat agtaaaaaca aaaacaacga caaaactagc
     3241 aactaatatc cagttaaatc ctgtcaataa tcctataaga agtaatgtaa ttaatgcatt
     3301 tagacctgtt ctcccttttt catccataaa agttatttta gagatgtcga tattattttt
     3361 cacattattt atatgctctt ttacttgttc ttccatattt cttttatata gtatataaac
     3421 tacaccaact aaaaacactc ctatacctaa aaccatgttt aaattaaaag ctaccataag
     3481 caatattgaa ccaaaagcta actttaaaag cttaaaaaat acactatcta tctttagagt
     3541 tatatttctg atttctttac attcatctct taaagaatat ccacttgaat ctaagttctt
     3601 aatctttgtt tttacaattt cagaataaaa aagctttatt atcccattta acattttcat
     3661 cactcctaaa tattttacat atatttgtaa gttttacaat tataatattt tttatacttg
     3721 gtaataataa aaaataacaa atattttatt atttagtatt tattgtaaat aagttattga
     3781 agcttgatta
//
LOCUS       DAEMHW010000275         3780 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEMHW010000275 DAEMHW010000000
VERSION     DAEMHW010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.277"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(157..1200)
                     /locus_tag="KPK67_003587"
     CDS             complement(157..1200)
                     /locus_tag="KPK67_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBG2460502.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            complement(1221..1667)
                     /locus_tag="KPK67_003588"
     CDS             complement(1221..1667)
                     /locus_tag="KPK67_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBG2460503.1"
                     /translation="MKLIWLIVAILFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            complement(1837..3417)
                     /locus_tag="KPK67_003589"
     CDS             complement(1837..3417)
                     /locus_tag="KPK67_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBG2460504.1"
                     /translation="MLIGTSKLSRNVDTACVEKVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
BASE COUNT         1199 a          701 c          340 g         1540 t
ORIGIN      
        1 ctgttaattt atttttataa tttcatcttt ctaataaaaa caaagaagaa gacttaaaaa
       61 tttataagtc ctcttctttt caacgtgcat ttttgtgcta tatttaatta attcgacctt
      121 taatttccat atccttccta tgataacaaa actcttttat ttttttaatg attcattatc
      181 atttaaaact tcttttatat caagtacttc tttgttatca tcactcataa cttcctttat
      241 tcctttgaat gttcctagga aattaactgc ttctgaaggt aaaaccagtt ttgttgactt
      301 accttcagcc attttttcaa gagcttccat agattttaat gctagaatat tattgtctat
      361 atcagcctct ttcattgctg aaaaaactaa ttttaaacct tctgctgttg ccatttgagt
      421 tctttttatc atttcagctt caccttgtgc cttagcaata gctgtctgtc ttatagcttc
      481 agcttcacct tctgccacta atatagcaga ttctttttca ccttctgcta cacgtaccat
      541 agcttctttt ttggcttctg ctgttaatat agcagattgt ttttcacctt ctgcttgaag
      601 tattgcagct gacttattac cttctgcttg aagtattgcc tctcttcttt ctctttctgc
      661 tctcatttgt ttttccattg caacttgaat atcctgagga ggcattatat tttttaactc
      721 tactctattt acttttatac cccatttatc tgttgcttca tcaaggatag ttctcatttt
      781 tacatttatt atatctcttg atgttaatgt ttcatctaaa tcaagttcac caattatatt
      841 tcttagtgtt gtagctgtta aattttcaat agcagctatt ggattagcta tctcaaacac
      901 atatctaact gggtctgtaa ctttataata tacaactgta tcaatctgca tagttacatt
      961 atctttagtt ataactggtt gaggtggaaa atctattaca atttctctaa ggtcaattac
     1021 atatgccatt ttatctaaaa atggtattaa gaaatgtaca cctgtttcag ccactttttg
     1081 aaacttacca agtctcatta ttatacctac tttagattgt tttataactc ttatacaagt
     1141 taaacttatt gctacgacta ctactattaa tacaatactt aaaacaattt tagtacccat
     1201 actataaatc ctcctttatt ctattggttt gtcacattaa ctgtggcgtc tactttttta
     1261 acaactaatt ttaccccttc aatttttaat acttctactg tagaaccttt ttctatcttt
     1321 tcaccatcta tagaaatagc cgtccatgtt tcatgttcaa ctacaactag acctgtgtta
     1381 tttggagaaa tctcttctgt aacaatacct tttttagaca tcattgcttg taaattagtg
     1441 ttagatttat agcctttatc tttttttact atttttttag tagctacgac taacattgct
     1501 atagaaatta ctgcaaatat taaaatttgt aagaaaatac tctctataaa gctacttaaa
     1561 aatataagta ttacagctcc tacactaaac catatcaatg ttaagcttgg tgttaacatt
     1621 tctgctattc caaataagat tgctactatc aaccatatta acttcatact atctaccctc
     1681 ttttcatatg aattataaaa ccctatataa attatatgtt tatacaatgt ttttgaatat
     1741 aaatttaaat tacaaaacaa taaaataaat taattaatag tattatatca acatactttg
     1801 ttaaaattgt tacaaagtag tatcaaatat caccaattat atatccaaca actctttcat
     1861 tgttaatgaa atgttactta ttaaatccgt agcatttacc gtatacattg ctttgctcaa
     1921 tttatctcct atatagccat gtatatatgc tccactaact gtagcttcca atatatccat
     1981 accttgacct ataaatgaag taatcattcc aagcaaacaa tcacccattc caccagttgc
     2041 catacagcta tttcctgtag gatttatata tgttttttta ccatctgtta tgacagtttg
     2101 ataacctttt aaaagcacca ccagattata tttttgagca aattcttttg caacatctac
     2161 tctatgttct cttatatacc caattgaatc tcctgtcaac ctggccattt ctcccaaatg
     2221 aggtgtaatt acaaatctgt ttttccattc taaaaactta taacatctgt ccttcataac
     2281 gtttaatcca tctgcatcta atactattgg gcaattagaa ttttctacta tcttaagtaa
     2341 tttatcaaat gtttgactat tatctcccat acctggacca aaaccaattg catcactaga
     2401 attaagtaat ttatttaatc tttcttcttc aaaatttaaa gtcattgctt ctgtaagtct
     2461 aatagatacc ttttcttgca catcttcact actaattaat gttacaagtc cactcccact
     2521 cttaacagca gattcagttg ctatgaaact tgcaccataa aatccttttg aacctgcaac
     2581 tatagatact tttccaaaat cacttttaaa accatgctta tctcttcttc taatattatt
     2641 ttttacaaaa gtcttatcag ttatgaactc ttcatcatgg tattttcttg taataaaatc
     2701 tggaacacct atcttttcta caactacatc acctataagt gtgctagtgt catagtttag
     2761 aaaacctctt ttataaaact caaagctaat cgttttgttt gctctaatac aagttcctaa
     2821 tacatttcca ttatcgctat ctatacctga aggaatatct atagaatatg tatttttgct
     2881 attatcgttt actatgtcaa taacttcttt aaaaatacca gttatatcac gttttaatcc
     2941 agtcccaaat atagcatcta ctacaacatc acttttagat actctatctt taaaaattgt
     3001 taaattttta ttttttataa tattttgttt ctcaacttta ataattttat ctacttcatt
     3061 ttcatctcca taattttttt cacttaaatc atttttatta atatttataa tattaattcc
     3121 catatttaaa agtacattgt aatttatttt agagcattca cttagttttt caagattacc
     3181 tactaaaaat acgtttactt cttttccata tgtatttaga tgtcttgcta ttccaagacc
     3241 atctcctcca ttatttccaa caccacatac aactgtataa ctattataca tgtctatatc
     3301 catatttttc attgcagata taaccgcatt ttccatcaat acaatcattg gaatccctac
     3361 tttttctaca catgcagtat caacatttct gcttaattta gatgtaccta ttagcataaa
     3421 ttatcactcc tataaagtta aagacttctt aaatacttac caaaaatatc tattgaaatc
     3481 tttttaatcc cataaatatc ataaataaaa tcactaaaac taagagtttt gaaataattt
     3541 aaaataacaa aaaattttga attaaaacaa ttatttaatt tattatacca tataatttaa
     3601 gttattatgt tgatttaaaa cctatttaca ctaattatct aaatatctct ttatttttat
     3661 aatcttttat gacttattta tatttttcat ttttataaaa aaacaataat taagaatata
     3721 actatattct acatgtaaaa atttttttat ttaaactcaa ttttctgata atttgataat
//
LOCUS       DAEMHW010000276         3775 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEMHW010000276 DAEMHW010000000
VERSION     DAEMHW010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.278"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            169..993
                     /locus_tag="KPK67_003590"
     CDS             169..993
                     /locus_tag="KPK67_003590"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG2460505.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            1033..1563
                     /gene="lepB"
                     /locus_tag="KPK67_003591"
     CDS             1033..1563
                     /gene="lepB"
                     /locus_tag="KPK67_003591"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG2460506.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDENEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(1645..3426)
                     /locus_tag="KPK67_003592"
     CDS             complement(1645..3426)
                     /locus_tag="KPK67_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBG2460507.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     IGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         1298 a          479 c          488 g         1510 t
ORIGIN      
        1 ataactttta agtttatttt gttatattat gaaggtttta tgaaaatatc aatcttccat
       61 tgtgcactac acagtactat ggtattataa gttagtaaaa ggtggcattt ttttattaat
      121 ctatgaataa aatatgctac tataaatttg tattggaggt attttgattt ggaaacattt
      181 agtttgcttg aaatttttaa ggcagtcatt ttgggcatag ttcagggaat aacagaatgg
      241 ctaccagtaa gtagtacagg gcatatgata ttagttgatg agtttataaa attgaatttt
      301 tcaaatacat ttataagtac atttttagtt gtaattcaat ttggttcaat attagctgta
      361 cttgttatat tttttagaaa attaaatcct tttgatagcg caaagaatat taagcagaaa
      421 aaagaaacag tccgtttatg gcttaaggtt ataattgcag ttataccatc tggagttatt
      481 ggaatactgt ttgaagatga tatagataga ttattcttta attcaacagt agttgctatt
      541 gctcttattg tatatggtat aataatgata gggcttgaaa agagaaataa aagacctaag
      601 tataaagatt tttctcaagt tacatataaa ttagctcttt gtataggatt attccagtgt
      661 ctagctctta taccaggtac atctaggtct ggttctacta taataggtgc agtattactt
      721 ggtacttcta gatatgtagc agcagagttt tcattcttct tagccatccc tacaatgctt
      781 ggagctagtg cattaaaact tctaaaagct ggatttggat ttactggatt tgaatggctt
      841 attttaggtg ttggttctgt agtagcattt gttgtttcaa ttgtagttat taaatttttt
      901 atggactata tcaaaaaaca tgacttcaaa gtatttggtt attatagaat agtcttgggt
      961 atagtggttc ttgcttattt ctttctatta taatgtattc taatttaata acaaatgata
     1021 aggagaaaag ttatgagtgt taaaaaagaa atatttgatt ggattaagtc aatagctatg
     1081 gctattgtac ttgcatttgt aattctacaa tttataagac cttctattgt aagtggagaa
     1141 tcaatgtatc ctactttaga tgataaagat tatctgattt taaataggat atcatacaag
     1201 gttggtaaac ctgaaaaagg cgatattgta gtttttaaaa ccaatttagt tgatggagaa
     1261 acaggaaaga aaaaagactt aataaaaaga gttatagcta ctgaaggtga cagaataaaa
     1321 atatcaaatt ctaaagtgta tgtaaatgga aaattattaa atgaaccata tatacacaat
     1381 aactatactt ctggagatat agatactgtt gttccaaaag gtaaactatt tgcaatggga
     1441 gataatagag aaaatagtaa tgatagtaga ttccctgatg taggtatggt tgatgaaaat
     1501 gaagttcttg gtaaggttat ggtgagacta ttacctcttg ataatattgg gaaagtagac
     1561 taaaaaataa gagactgaat tggtaaagtt tattttacaa atctcagtct ctttttattt
     1621 tcttaaatta caaatatttt tttactaaat atcttttgat tctgtaataa gtcttattat
     1681 atcacttgct tcttcattaa aagttgttac tatacttagg tagtctgttg ctctactcat
     1741 agctaaataa accttattta aatctcctac aaagtcattt acttgataat cttgttcaat
     1801 atcatttata gtttgattgt ataacatctc taattcacat actattaccg ctttatactc
     1861 taagttcttt attgcatata tatttgctat tgtagttcct atcttcttac ttatatttgt
     1921 caaattatct tctgcacata tataaggaat ttttgcctct tccaatgctt tttttaacat
     1981 atattgaaag taaatggttt ttccactttt taatttcttc ttattgtaag gataaacgat
     2041 agcaacttca gaataatcaa gacctttttt acttattaaa tactcaattt cccagattac
     2101 agaacttatt tggtcatcaa ggtcacttac ttttattata tcaacagact tatttccacc
     2161 tctaagtgct ttagtataaa agaaagtact aaattctgta tttggtctta attcgttgat
     2221 atatgagtta gaattatttg aaaatttatt tgtaaaatct acaatctctt tagcttgtct
     2281 ataattttta cttaagataa tcttatcatc aaattctata ccattatata attttttaaa
     2341 tatattcaat ctattagaga tattcaagga gttacaagaa cacacattaa atatgaattt
     2401 tgttttatat aaaaactccc ttataaaatc tatttcatac tctaaaaagc tttctgcttc
     2461 atcaataaaa atagccttga acatattttt attttttata atgttctgtg cttgttttac
     2521 aagattatta aaggtctttt cgtaatcatt ttttaacatg ttataatcta caattaaatt
     2581 gaatttttta gctagtttaa atataaaaga actaaatgtg tgaacttcta aattgttatt
     2641 atctttgtat aataactcta tcctctctcg aagctcattg cacaattgtt ttgtatgtgt
     2701 aaatatgact aatttatgat gtggatatac ttttgctaat tttatagccc tagaaagcat
     2761 aattgctgtt ttgcctatac cagaaccacc ttcaataagt gtattcccat atttaataga
     2821 aacaacatct ttaatttgaa cgtcttccat catagttgct gtatacttat agtcatcatt
     2881 gtagaatgaa atttttttaa atttttcatt tagatgaagt ttatcattta ttatatagta
     2941 ttctgaacat atgtctagta aaaataaatt taagtttatt tcatcattct catcattaag
     3001 atattcctcc agcgaaccct tattcattat atcttgaagc ttatttttat ctattatatt
     3061 attattaaca aattcttcaa attcatatgt ttcctcaact tcaacgtatg gcataatgta
     3121 aacgtagtta taacttatat ttctattctt ttgtcccatt tttagtttca aaagttcata
     3181 ttcctcattc ataacttcaa gtaactcttc ttctaaaata aaaaatatat cttctgttgt
     3241 atccataaat tttataaata aaattcttcc atctttaaca tacaacaaat cggtatttat
     3301 tcctttaaaa ggagtaactt ttggcactac tttaacgtcc tcattgatat tttcgtaaaa
     3361 tattttctcg aactccttca attttagggc gtctaactga gaatttatat ttatgtattg
     3421 gctcacttac attacctcct tctgttatac ttaattatac cattttgaac tatattttta
     3481 tatatacttt ttataggcaa atttagtaaa taaattcaaa ataaatacaa ataaatacaa
     3541 aactgctaaa ataattttgc aataaatata gatatttatt gttatagaat actttctgta
     3601 aataataaaa cgataaattt cataattaat ttttaaaata ataagtaaag ttttaaaaaa
     3661 aataaatctt gttgactaaa aataagattt attataaaaa atttaaaaac ctgctaaaaa
     3721 taatctattt tcattagcag gttttattta taatcttctt atattaatac tatta
//
LOCUS       DAEMHW010000277         3744 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEMHW010000277 DAEMHW010000000
VERSION     DAEMHW010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.279"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(154..690)
                     /locus_tag="KPK67_003593"
     CDS             complement(154..690)
                     /locus_tag="KPK67_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBG2460508.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(708..2021)
                     /locus_tag="KPK67_003594"
     CDS             complement(708..2021)
                     /locus_tag="KPK67_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBG2460509.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2376..2816
                     /locus_tag="KPK67_003595"
     CDS             2376..2816
                     /locus_tag="KPK67_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG2460510.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(3001..3189)
                     /locus_tag="KPK67_003596"
     CDS             complement(3001..3189)
                     /locus_tag="KPK67_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG2460511.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKKE"
BASE COUNT         1288 a          646 c          359 g         1451 t
ORIGIN      
        1 ataaaaataa aaaaagaata ggataatttt cctactcttt tttctgtact tacatataat
       61 attaaaactc tttatttttt aatatatatt ttaaattttc ttttttaata aatctttctt
      121 ttttaataaa tctttctttt ttaataaatc tatttaattt tcttcttcta tatcagagcc
      181 aaagggaatc catgcattac caaactcttt atctttaaat aaataagcta atccagttat
      241 ttgcttcaat ctaagaattt catcaacttc catacccagg tgctttgata tccattcatc
      301 agattttcca atttcacaaa gttcagttat aattttactc attaaatcca cattatgaga
      361 acctcttgca cgattgtgtc gaatagtact tgccattctt tcatcaagag atttatcaat
      421 tactgaaaca ggaagaagtc cattttctct atcatatata tcctcatttt caagcattac
      481 tctatatcta tgaaaaccat ctacaataat atacttatcc ttttcctcat tataataaca
      541 gacaattggc attgtatacc catcttcttt tatactttta tacaacaact gcatttctgg
      601 tggggctact gcatttggat tatagtcgtt agattctatc ttttcgattg gcactgcgat
      661 gattccatat acaggacttt tatattccat agcatcttaa ctcctatcta atgaattttt
      721 tcatatttct tcttaatata ttccgttcta attctctgtt cttttgtagg accaaatccc
      781 ataaatctac acatatggtc attttttaaa atacaataac acattctttt ccaacttggt
      841 atatctttgg taaatttaat atcatccgta tcatcaggta tatcatccaa aaatatgact
      901 ctactctttt tatcttttgt atgatttgaa actccattta attctatttt ataacctttt
      961 ctcaacaatt cacgtataac tatttctgac aaaccaccac ctgtattctg ccaaaactca
     1021 atagatgttg aaaatttttt aatatagcta ttccttatat tttcaggtaa ggtatcaagc
     1081 aaaaaatatg tgaaactttt ccatgtgtgt ccatatggca atttaacact acgatatccc
     1141 atagcttttg ttttgccata aatagaagta aaattaactc cttgtactct agcaaccaat
     1201 ttcacccata catctgggtc caatattctg tataaattta aactctcttt tgctgaatca
     1261 ttaaaaggtg atgccactct catttttctt ggagctactc cagctttata gaataagtca
     1321 taaattttat tatagtcata gtcaaattta tagtgtgcta accatatatc ttctatttcc
     1381 caatcatata taggagatgc cgtccagaca tttttaaatt ccttagttat ccagcacttt
     1441 cctttatatc catgctgttt attcacaata gaacaataac gttgtagaga ttctgctgct
     1501 cgtgttccta ataagcaaac aacttttgca ccttcatgta tatctctaat ccatctacca
     1561 aattgttttg ccaaactttc ttgtctcatt ttatatttat aataagtaaa aggattattt
     1621 ttttgattta ttatgtattc tttgactggc atttctctta cccataactc ttctttctca
     1681 tcatcccatg gataccaata catttcataa tttcctacag cagttcttgt agccattggt
     1741 aaacacaccc aatatggttc aatatcttca atattatctt caaatacttt ttcaacatat
     1801 tcagtagtca aactatattg agcctcaaaa tcttgatgaa atactcctat tttggaactt
     1861 atattattct ttctcttata atccaaaact aaatttagca gtaaaccact atcttttcct
     1921 ccgctaaaag atacatagat ataatcaaat tcattaaaaa gataagaaag tctttcttga
     1981 aaagcttcaa atacatcgga ttctaaatat ctcttatcca tttgaattat cccctttcaa
     2041 ttagaagttt ataaaacctt acaatgcaat attattatgc ttttttagca gtaatcagca
     2101 taaattatac attatttaaa tacacaaatc caactaaata catataatta taatgcaatt
     2161 atacttttgt attataaact atattttatc attattttac atttttttcc aatatatata
     2221 attatcatat ttttctatta gctaaaacta attaccttat atttttacgt attggcataa
     2281 taatacataa aaacttgaat aatgcataaa aacttgacat actgttgtca taattacttt
     2341 gctataatta aaagtaaaaa tggaggtaaa ttctaatgta tgaacgtatg cttgataaaa
     2401 gcaataaacc ctgtttgtct caaataatag aatatatagg taaagatgga tatgcacgat
     2461 tgtgtaagtt ggaaacttac ttgaaaactc aatataattt atcaagagaa cttcgatttc
     2521 cttttggaaa caattatggt tggggttaca aatatagcca taaatctttt catttatttt
     2581 atgctttttt tgaaaaaggt gcatttactg ttctgattca gataggtgac aagcaagttc
     2641 cagcagtaga aaaaacgttg gtagctttga caccaaaagc taaagaactc tgggtcactc
     2701 gttatccttg tggcaataat ggaggttgga ttaagtatcg tattttagaa gatgcagact
     2761 tacttgatat tattcaatat ataagagcaa aaaaatctcc acctaaacaa aattaacttt
     2821 tattttttgt gatttaaata tagtgtatac aaatttcatt ctttccatat aaaaaaaata
     2881 atataaaaaa tatctattag tataacttat ttagtatgtc ttattctact ttttacagat
     2941 aattaaaaag ttttatcata aataaatcat aactagacat cctagtaaat caaaattaat
     3001 ttattctttt ttattagaat ttttgataat agtccctata catatgccaa ttatcattcc
     3061 aaaaattaga ccataacaca ttgtgatacc tacatttcct ttagaaatac ctatagcaca
     3121 acctatagcc attccaagta tcatgcctat aaacatacat ataataacta aattgttgtt
     3181 tttattcata gtttttctcc attcaacata ttttaaattg cctagctcgc cttcttagct
     3241 taaaacaaag aaatataaat taactttctt tcattctaat atctgactgt ataaatgtca
     3301 aacaacacat ttcttgtcat aacctcctct ttttgaagta aatacaatct cccagacgca
     3361 ataatatcaa tttcctaaaa ccatttaagt aacttttata tttacataat atttacaatc
     3421 ataaactata ttgttattca tttatacatc ataaacactc cctcctaatc attattatac
     3481 ttaaaataat gttcatttat aataatatat ctaacgcact ttaattgaaa aaactaaaac
     3541 tttgcctttc attcccatat ataaataagt atactttttg atgctctatt acaaatcaac
     3601 taaattaatt gatataaatt atcattttct gttgaaaata ccattcgcac atgctatact
     3661 taattgcata aatggtaata aatgtcatat gattttacaa atctattaat aacaattatt
     3721 acttttactg tattttaaat tttt
//
LOCUS       DAEMHW010000278         3692 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEMHW010000278 DAEMHW010000000
VERSION     DAEMHW010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.280"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(125..892)
                     /gene="rsgA"
                     /locus_tag="KPK67_003597"
     CDS             complement(125..892)
                     /gene="rsgA"
                     /locus_tag="KPK67_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HBG2460512.1"
                     /translation="MNGMKQINLQGYGLNRNFITQSMEYHDLYLARIISQEKNFYQVV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCAIWEAIAHQELSPKRWES
                     YRRLKTEAVFADNKSEYLAKKEKKFKNISKINKTIRR"
     gene            complement(889..1290)
                     /locus_tag="KPK67_003598"
     CDS             complement(889..1290)
                     /locus_tag="KPK67_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBG2460513.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      2086..2182
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2236..2412
                     /locus_tag="KPK67_003599"
     CDS             2236..2412
                     /locus_tag="KPK67_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460514.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            2822..3460
                     /locus_tag="KPK67_003600"
     CDS             2822..3460
                     /locus_tag="KPK67_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460515.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
BASE COUNT         1400 a          509 c          477 g         1306 t
ORIGIN      
        1 taaattactt taaataatac cataaaccaa atttgccatc aaaatcacat taaagtaaca
       61 aattgatggc aaatttaata tattaatagt atatagactc atagaatgaa caaaaaaact
      121 acacttatct tctaatcgtc ttatttattt ttgagatatt tttaaatttc ttttcttttt
      181 ttgctaagta ttcgctttta ttgtctgcaa atactgcttc agttttaagc ctacgatagg
      241 actcccatct tttaggcgaa agttcttgat gagcaatagc ttcccagata gcacagcctg
      301 gtttaatata cttagaaatc tgtttataac catctggctc tatagatgaa gtaacaacta
      361 catctacacc caaggctata gaattaacta cttgtaattt acgatgcaca ttgtcacata
      421 agtctgattt agtaagtact actactggga tagctccact ttcccatgtt aaagacagat
      481 acctctccaa tctatgaatg ttaaaatcct tattcagtga catgcacaaa aaaacaatgt
      541 caatattagc tgcaaccacc tgttcctcat ttacctttcc agcagcctta cgcacaaaca
      601 agctttttct atgaagcaca tgatgaataa tagcattacc atttttatta ttgcaacgat
      661 ccaacataac gaaatcacca actgtgggaa aatcagattg tatagtagcc tcaaaacgca
      721 gttttcctga aacttctgca aacagtacac catttacaca tacaacttga taaaagttct
      781 tttcctgcga gataatccga gcaaggtata aatcatgata ttccatactt tgagtaataa
      841 aatttctatt tagaccatag ccttgtaaat tgatttgttt cataccattc attctgtttc
      901 ctccttttcc aacttttcaa tgattctttc tagtgggaaa gggggctgtg ccagtggttt
      961 taatgcaatt gacattaatt tcactgaatt atcatcagct gcaatacgat tagaactaag
     1021 atgtccacag cgtgtacaac gatgaataat tgcccactca ccattttttc tcacccacac
     1081 tccaataggt tccatattag catcacaatc tgcagcacgg tcaccaggaa tgatatctac
     1141 atgaagacta tgtaaacaat gtggacaatg atttctatgt tgtgtaccag ctccttcagg
     1201 aactacaagg caaccacaat ttttgcaaat aaagttatcc atacaggaat tttcctgata
     1261 ataagctcgt tttctatttt tataattcat ttttatttcc tccgtatcgt gtattttcta
     1321 caaaaaaagt ccacaggtat ttatatctgc ctttttatgt ggtatacata tttacctaca
     1381 caaaaacaga aaacccctgt ggactagaaa atttagtatc ttctatttca acataattat
     1441 attcaaaata atactgtgta aaattaataa aagtgtagca gattaaagcc cactacacca
     1501 caatttaaca aagtaatatg taatattatt atttgttgtc taatgttagt tatctataaa
     1561 ggcagagtaa gacacaacaa tcaaatgtat caaaaataca cttaaaaatt ctgattaaac
     1621 agaacccata tgcgtctcca actatttatt tgttggttac cattatatat aactctgaca
     1681 tcaggcaaga cactccttca ctaataatta ttattgaaat tttatcataa aatataagta
     1741 tgttcaagta taatttaata tattcatata tttatgtttg tctcattttc ttattctata
     1801 aaatctagtg gtattttaaa atttaatgtt cacctagtat taataataaa aattaactgc
     1861 tattatttgt tttctcattt tatgcctatt gctcaattta tctcacttaa attaataaca
     1921 actgtctttt catacaaaac ctacaatcaa gtttttccat atattctaca tgcatagtct
     1981 atttttttga tataaaataa cgttcatatt tgtaaattta tttaattttc aaataagtat
     2041 attgaataat aagcaattct attgtattat tgtaattgta gaataaaact aaatcggcaa
     2101 aactagagaa atttagtgac gcaaagctat agggactaac gcttataaaa taaactatga
     2161 gttatgtcag ccagttgcca aaaagagata ttctttttgt ttttatgaaa gttttttagg
     2221 gggataataa tatatatgtt tagagataaa atggatagat gtacacatat gttgactgct
     2281 tatattggta gttcatatga ttattgtgat tttatagata cacaactaga tgattttata
     2341 ctagagtacg gagaaaatgt ggtagaatct tgtttgcatc aagtgatggt attagtaagt
     2401 aagtataatt aaatagattt ggttttctag agaacccttt ttagttttat ctactttaaa
     2461 tagccttatg ttagaaagct aattaagata gatttctagt attcataatt ttatttttta
     2521 tttaaaatac atataataaa gatgtaaatt tgtatcaaat aataaatatg agaacttaaa
     2581 ttctcttatc tatgtgaaaa taaacatctc ttaaaatgaa attctgcaaa cataaatgat
     2641 tttttagttt gaaggattag attaacaaaa ttataaattt gcaaataagc tatattaaaa
     2701 aatataataa ttttttatat aacttattct tttataactt ttaattttaa aagaggagaa
     2761 tgtatataag agtattcaat gtactagaaa aaatcaaaag aaatattaga agaaatggat
     2821 attgagtaat caaagaaaag attttgatat tacaaatgat atgtttcaag aaagcattcc
     2881 tatcacaaca aaaattattc ttgaagacat gccttcaaat gatgaattaa atcatgtttt
     2941 ttcaaaaggt tgtgaacgaa aaatgaaaaa gagaaaaatt gtattaatca cactactatt
     3001 aataggtgtt ttgttattag gaagtatcct atataatctt tttttaggaa aaacagctaa
     3061 tatttcaatg ctaaaggaat cttggaattt tgatatacca atacctaaca aagaaattga
     3121 agtatttgat actcaagata gtattaatgg agatggacaa agttatttta ttcaaggatt
     3181 ttctgaaaaa aatttcaaaa aagtttttaa tttaaaaggt ggtatagttg taagcaaaga
     3241 taatattaat gaaatagaaa aatatattga taaatttaaa agagattctg ttaacattaa
     3301 taaaagtaat aaaaataaaa ttgaagaaga ttttaaaaaa tataagttag aagttaaaaa
     3361 agatgataag tatatatata agagaaatta tgagaactac gtagttttaa ttataaaaaa
     3421 agatgaacaa aaattataca gtttaatatg gaatcagtag gtacatatgt attattaaga
     3481 attataaata ataaaaataa attttagtca atatacaaaa accttttaaa ttgtctggaa
     3541 atcacctctt catttgttat aaaaacatac aacaaatgaa gaggtgattt tatggtttta
     3601 aaaaaaataa ttataagtgg atatgtgctt tcacatgttt atcaacattt gtaatgccta
     3661 tatttgctaa tgaattataa tataaaaata tt
//
LOCUS       DAEMHW010000279         3617 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEMHW010000279 DAEMHW010000000
VERSION     DAEMHW010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.281"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            128..1504
                     /locus_tag="KPK67_003601"
     CDS             128..1504
                     /locus_tag="KPK67_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBG2460516.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1815..2486
                     /locus_tag="KPK67_003602"
     CDS             1815..2486
                     /locus_tag="KPK67_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBG2460517.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2675..3472
                     /locus_tag="KPK67_003603"
     CDS             2675..3472
                     /locus_tag="KPK67_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBG2460518.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEYLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1364 a          402 c          673 g         1178 t
ORIGIN      
        1 attactatca ttgtctttat acaatattta tactataata gaataataat ttagaggaaa
       61 gctctaaaaa taaacaaatt aagggtctta acagtagtga ttttatgtta aggaggtata
      121 aagaaagttg aaaacaatag ttagaagact tattctctac gcagatggaa tgcgtcctac
      181 tcaaataatg gtatctgggt ttgctgcgat tattgtaatc ggagcattgt tgttaacact
      241 cccgatagcg tcgcagagtg gagagagtat aggcttatta aatgctctat ttacggctac
      301 ttcagcggtt tgtgttactg gtctagttat ggtagataca gcaacttatt ggagtttatt
      361 tggacagata gttataataa cattgataca aattggtggt cttggattta tgaccgtagc
      421 cacaatgttt tcacttatgg cacgaaaaaa aatacaacta agagaaagat tattaataca
      481 agagtcatta aatcaagctg atttatcagg acttgtaaga cttacaagat ttgtattaat
      541 aataaccata acaatagagg gaataggtgc actggtttta tcaactgtct ttattccaca
      601 atttggtttg tctaaaggga tatggtatag tgtatttcac gcaatatcag cgttttgtaa
      661 tgccggattt gacttgatgg gcagtgtaag tggcccattt acatcgttaa attcttatgt
      721 aaacaacttt acagtatcaa tgactgtttg cgcacttata gtgcttggag ggctaggatt
      781 ccctgttgta cttgatattg taagaaaaag aagattttca aaattaaatg tgcattctaa
      841 ggttgtattg ttttcaactg ctacacttat ttttgtgggt gcattattta tttttcttat
      901 agaatttaat caaaaagcta ctatggcgga cttgccttta aagggaaaag tactatcagc
      961 aatatttcaa tcagtaacag caagaactgc tggttttaat acgcttgatt tagctacttt
     1021 acgagaaagt agtgtatttg taatgataat tttgatgttt attggagcat cacctgcatc
     1081 aactggtggg ggtataaaaa caacaacact agctgtttta atcattacag ttagaagttt
     1141 tctatcagga aaatcagata tagaagcttt tgaaagaaga ttagctccat caactataaa
     1201 aaaatctctt ggtatatttg taatcagtat atcagcagtt atttttggaa cattgattat
     1261 ttcaataact cagcctaatt ttacattagt tcaatcagct tttgaggtta catcagcact
     1321 tgctacagtt ggttcaagtt tagctggaac tccaaactta aatgctttag gtaagattat
     1381 aataataatc tttatgttta tgggtagagt tggttctctt actttattta tggctatact
     1441 ttctggtggt agaagaaaga gtcaaccaat cagatatgca gaaggtaaaa ttatggttgg
     1501 ttaagttacc aaggagtaat ataaatttat aatggattta gagtagcact gtaaatctgt
     1561 tgataaaata aatgagccta gatgtaatta tatgaagaaa taaaatcgac taatgaacaa
     1621 aattaattaa tgaaataaaa aaacagggaa ataaaatacg aataaaaagg taaatcaaaa
     1681 ttaaattaat ctgtgtatat aaaattatat attgtgtata agttttgtat gtgtaaagta
     1741 tataagtttt gtatgtgtaa acaattaatt taagagatat tatataaatt gtgtattagg
     1801 gaggaagttt cgagatgaag caatatatag tcattgggtg tgggagattt ggaagttcag
     1861 ttgcgtctac tatgcatctt ttaggacatc aagtaatggc aatagacaaa aatgaagatt
     1921 cagttcaaag tatatctgac aaggtaaccc attcacttat agtggatgtt actgatgagc
     1981 aagcgttaag gtcattaggt ttaggtaact ttgatgtagc agtagttgca ataggttctg
     2041 atataagggc atctataatg gcgactctta tagccaaaga aatgggtgta gagttgataa
     2101 tatgtaaggc aaaggatgaa ttacaagcta aagtgcttta taaaattggc gcagatagag
     2161 ttgtatttcc agaaagagat atgggagtaa gagttgcaca caatttagtt tcggataata
     2221 tattagacca tattgaactt gacccagagt attcaattgt tgaaatcgta actccaaata
     2281 gttgggttgg caagacactt atagagcttg aattaagagc tagatatgag ataactgtac
     2341 ttgctataaa aacaggtaaa aatataaatg ttacaccttc tccagatgag gaacttacag
     2401 ccggaagtat cctagttata atcggtcaaa atactagtat aacagcgata acatctggaa
     2461 ataaggggat aattagaaga agataattta ctatttaata tatatttaat tgcaatgaaa
     2521 gtagatagta tcatataatt atgaatagtt atatgatact atttttttaa tcgaaggtag
     2581 tatttatttg taagattaga taaagagaag ttaaattaaa agtaaggagg ctgtgctaat
     2641 ataaaaattt ataagttatt agcatttaga taaaatgatt acaaatataa acagtaagga
     2701 taatgaaaag ttaaagtata caagagcact attaaaatca aagaatagga ataaagaatc
     2761 aaagttcata atagagggat acagaatagt aatgcttgca cttgaatgta tggcaaacct
     2821 tgattatgta tttatcaatg aagaatttga aaataagaaa gaacatgtaa aactattaga
     2881 atatttggat aaaaaaaaca caaagatata caagactact aataaaaact ttaaagaatt
     2941 agtggataca gaaaatactc aaggaataat aggtgtagtt tcatttaaga aaaaaaaatt
     3001 aagtgaaagt ataaataaaa aagataaatt tgtattgatt ttagacagaa tacaagaccc
     3061 aggaaatatg gggactataa taaggactgc tgattctgct ggagtagatg ccataatagc
     3121 actaaaggga tgtgtcgata tatacaaccc aaaagtaatt aggtctacta tgggttctat
     3181 ttttgatatg aatataattg atgcttcaca agatgaaact gtggacatgc ttaaatcatt
     3241 ggattttaat atagtttcaa gttacttaaa tacagaaaat ttttatgaca aaatagatta
     3301 tggctcaaaa gtagcattgg tgataggaaa cgaagcaaat ggaataaatg aagaacttgt
     3361 atcaaagtct gatattttgg ttaagatacc tatatatggt aaagccgagt cgttaaatgc
     3421 tgcgataagc tctgctatac tgatgtatga aataaaaaaa tacttaattt aatgtattgt
     3481 aataaatata atgtcatgtt ataatcttga attaaatagt agagtatcaa aaatagtcaa
     3541 tatatattaa aaaaataatt aaattaatta tatattagat gtatattata taaaaaataa
     3601 ttgagttagt tatgcat
//
LOCUS       DAEMHW010000280         3517 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEMHW010000280 DAEMHW010000000
VERSION     DAEMHW010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.282"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            46..954
                     /locus_tag="KPK67_003604"
     CDS             46..954
                     /locus_tag="KPK67_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG2460519.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            1098..2543
                     /locus_tag="KPK67_003605"
     CDS             1098..2543
                     /locus_tag="KPK67_003605"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBG2460520.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANVDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            2854..3306
                     /locus_tag="KPK67_003606"
     CDS             2854..3306
                     /locus_tag="KPK67_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG2460521.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
BASE COUNT         1486 a          376 c          584 g         1071 t
ORIGIN      
        1 gtaaaagtat agctatttta ttttatgaaa aggaaaggag ttattatgta tagtacaaaa
       61 atactagatg attatgagta tgaaactttt aatgtttttt ctacaaaatt aaatccaaac
      121 acgaaacacg attatttatc aaaggttata ctgtttaaag aatttttaaa agggaaagaa
      181 ttgatttatg ctacgaaaga agattgtaaa aattttgtag actatataca gactaaatat
      241 gcgaaatcaa cttgtgaaaa gatatatagt tatttacata gtttttataa cttcttaaaa
      301 aaagaaggat acatagatat aaatccattt agatatgtag aaaaacccac agtaacaaga
      361 ataaaaacaa aagatgatgt tctaagtata caagaaataa ataaacttat aggtatccta
      421 ccaaaattaa atataagaga tagagtaata atagtctgtc tagttactac tgggtgttta
      481 ttaaatgaat tagttagctt aaagtggaaa gaccttatgg tagatgaaaa cgataactca
      541 tatgtaaggt taggtaaggg aagaaaagaa agagtagtta aattacatcc atactttttt
      601 aaacttttag aagattacag gaattattct gggcttccag aggtaataat acctagtgat
      661 gattttattt ttacaactca aaagagtaat tctataacag atagaaatgt cagacttata
      721 gtcaaaaaag cattagattt agctggactt tctcaatatt cagcaagaga ttttagacat
      781 tcatttgcag caataagttt aaggcttggg gcagatgaaa gtgatgtaaa aaatcagctt
      841 ggatggagtg ataaatacta tgcaattaga tataagtatg tattaaattt tgttgatagt
      901 gaaatagtgg attacttaat tgaaaaagat aatttggata taaataaaaa atagtaataa
      961 ataaaaaata ctatatataa atgtgaacaa aaaataaaaa agtcttgtta tagttcgtaa
     1021 aattatatat aattatttat gtactaatgt gtacacaata atcacacgac aaaagattta
     1081 aggaaggtgc taatataatg atagataata aatatactac ttactcaaac ccacttacag
     1141 atagatattg tagcaaggac atgagttata tattttctcc acaatttaaa ttctctacat
     1201 ggagaaggtt atgggttgca cttgcagaag gtgaacatga gttaggaatc aatataactg
     1261 aagaacaatt agatgagcta agagctaatg tagataatat aaattttgat gaagctagaa
     1321 aaatagaaaa agaagtaaga catgatgtaa tgtctcacgt aaaagcttat ggtttacaat
     1381 gtgaaaaagc caaaggaata atacatttag gagcaacatc tgcatatgtt ggagataata
     1441 cagatgttat acaaatgaat gaagctttaa aacttctaag agtaaagctt gtaaatttaa
     1501 taaacaattt aaaagaattt gcacttaagt acaaagatat gccagctctt ggatttacac
     1561 acttccaagc tgctcaactt acaacagtag gaaaaagagc ttgtttatgg gctcaagatt
     1621 taattcttga tttggaagat ttaaactata gaatagataa catgaagtta agaggagtta
     1681 aaggtactac tggtactcaa gcaagttttt taagcttatt tgaaggagac cacgaaaaag
     1741 taaaagagtt agataagaaa atttgtaaaa agatgggata ccattcttcc tatgcagtaa
     1801 gtggacagac atatactaga aagttagatt atcaagtaat aagcatacta tcagggatag
     1861 ctcaaagtat gcataagatg actaacgata taagactttt acaaagttta aaagaattag
     1921 aagagccatt tgaaaaaaat caaataggtt catcagcaat ggcttataag aggaatccaa
     1981 tgagaagtga gagaatagca tctttatcta agtatataat ctctgaatct ataagccctg
     2041 caatggtaca agccactcaa tggttagaga gaacattaga tgactcagcc aataagagat
     2101 tggcaatacc tcaagcattt atggcagcag atgctatact tgaaataggg ataaatgtaa
     2161 ctgatggttt agtagtatat gaaaatatga taaatagacg tgtaaatgaa gaacttcctt
     2221 ttatggctac agaaacaata cttatggaag ctgtaaaaag aggtggagat agacaggaat
     2281 tacatgaaat aataagagaa tattctatga aagctgccta tagagtaaag catgaaggca
     2341 aggataataa cttaattgaa cttataatga atgatgattc atttaaaatg agcaaggaag
     2401 aaatactatc tataatggac ccaaaaaact ttataggtag agctccagaa caagttgtag
     2461 aatttgtaaa tgaagttgta gaacctgcta taaaagatta taaggaagac ttaggaaaag
     2521 tagatgtaga cctaagagta tagaattgta cagataaaat ataattaaaa acggctatct
     2581 tatttataaa taagatagcc attttaatgg gctagcgtct tctctccacc gctgtgaaga
     2641 gttcttctct acttactata ccatgaattt aataaaatag ctatataaat aatatttaat
     2701 aggaataata taaataaaaa taaaaaaatg ataagattac taattaaatt taaacattca
     2761 gcaaaaacta ttgtacttgc aatactttta atgtaaactg aaataaagtt atttagataa
     2821 tttaataaat tttaaaacaa ccgaggtgat tatatgaact atatagtaaa agataccata
     2881 agttttttag taaaaacatt tcctaagatt tattctagcc tgtacttaga agacctaaaa
     2941 aaatttgcac cagattataa tgtaaataaa acacaattaa gagcattagt tttcataaaa
     3001 aattatggag taataagtat gactgattta tgctctaaat taaatataga aaaaggcagt
     3061 ttaactagta tggttgatga tttaactgat aagaagtatg taacaaggaa aagagattta
     3121 gttgatagaa gaaaatatct aattgacatt actgaagagg gagataagat agcaactgat
     3181 tttatggata aattaagtga tggtttagga gaaaaacttt ctaaattaac tgaagaagac
     3241 agaaaaaaat atttagaagc aataaatact cttcaatata tattaaacaa agaagaattt
     3301 agatagaaaa aatataaaaa tttttgtatt taatggtact aagattaatt ctttgttatt
     3361 catttaacta tataattata agtctaaata caatctaaaa agtacatctt aaattttctt
     3421 gatgtacttt tttataattt ctctaactaa ggcaagacaa gttttagatg ctttaattaa
     3481 agtgaataaa gattactggc caacactata taaagaa
//
LOCUS       DAEMHW010000281         3460 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEMHW010000281 DAEMHW010000000
VERSION     DAEMHW010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.283"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..744
                     /locus_tag="KPK67_003607"
     CDS             <1..744
                     /locus_tag="KPK67_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460522.1"
                     /translation="LITLSLNLFEINKFKNSKFIMNLAIEDFGGIFYGLGILSNLGFI
                     QSILFFGILGLFIYYILMWKREFFSNNQSIYTLMMLPQNKFKICISKSIALITMIYGF
                     LISQTATLFICKYIFNFIFRNMPIINMNFAKDLYYLNLKSIPIDFISFMAIYVFLLLI
                     AISIVNCCILFIFSYINKFYVILITLLIIAGLYFSTIYINIITLFRKYYNLIGFQFNN
                     LVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            756..1955
                     /locus_tag="KPK67_003608"
     CDS             756..1955
                     /locus_tag="KPK67_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460523.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            1964..2776
                     /locus_tag="KPK67_003609"
     CDS             1964..2776
                     /locus_tag="KPK67_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460524.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     IFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            2787..>3460
                     /locus_tag="KPK67_003610"
     CDS             2787..>3460
                     /locus_tag="KPK67_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460525.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSNFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNIN"
BASE COUNT         1436 a          281 c          489 g         1254 t
ORIGIN      
        1 ttaattacac ttagtttaaa tttattcgaa attaataaat ttaaaaattc taaatttatt
       61 atgaacttag caatagaaga ttttggaggt atattttatg gactagggat acttagtaat
      121 cttggtttta tccaaagtat attgttcttt gggatattgg gactttttat atactatatt
      181 cttatgtgga aaagagagtt cttttcaaat aatcaaagta tttatacact tatgatgtta
      241 cctcaaaata agtttaagat ttgtatatct aaatcaatag ccttgataac aatgatatat
      301 ggttttttga taagtcagac agcaacatta tttatatgta aatatatatt taatttcatt
      361 tttagaaata tgcctataat caatatgaac tttgccaaag atttatatta tttaaacttg
      421 aagagtatac caattgattt tatctctttt atggctattt atgtattttt actacttata
      481 gctatttcta ttgtaaattg ctgtatatta tttatctttt cctatataaa taaattttat
      541 gtaattttaa tcactttgct gattatagca ggcttatatt tttcaacaat atatataaat
      601 ataatcactt tatttagaaa atattataat cttataggat ttcaatttaa caatttagta
      661 gcagggttat taagtttttt aattataatg tttattctaa atggaatatc ttatttgcta
      721 attaaaaaga aagtatctct ttaaggaggg gtggtatgaa attaaaatct aaaatatcta
      781 tacttattat atttttgact ttagcaatat attttagtat aggtttatta aataatgaag
      841 ataatttaaa gataaaaact ataaaaggtg actctaatga attagaccat ataaacataa
      901 attttggata tgctttgaat ggatatataa attcatatta tacaataaat ggaagtaaca
      961 aaaccaaaaa gactgaatta ataaaactac aaaaggatag taagaacaca aataatttaa
     1021 ttgatgtatc tagtggttca gatttaattg ctcatgttgg atttgaaatg aatgaggagt
     1081 caaaagatat aaactttgta atagatgaga aaaaagaaga tttagaaaaa ggtgtatttc
     1141 catcagaagg tagtgataaa gaaattgaat atgaaaaatt agatatgaat aattttacaa
     1201 ggacaagata taaagtagat aaaagtacag atgattatta tgcaaagata ctagcatcaa
     1261 aaagatataa aaaatactta tatgtatctg tactttatgc tacaagtgat tcatcagaaa
     1321 ataacaaaga atattcaagt actatagata taataaaaat taatccagaa aataaaaaaa
     1381 tcaagactat aaaaagtata aatttaagta attatttttc cgaaaaatac caaaacatag
     1441 agaacatagg tgtaagttat gctagcaaat atgaggataa gatgtatatt ttagtaaatg
     1501 tactaaatac aaacataggt gataatagta attctaaatt tcatttactt gagtataatc
     1561 ttgaagaaaa taaggaaagc ataatggaat taaaaactga tagcaaaatg gctttagcaa
     1621 aagcaaagtt agaagctaat aaattatata tggtaatgtc gaatgattat gataatagtt
     1681 caattacaga gttaaattta cttacctata atatattaga taaaagctta gatgaagata
     1741 aatatgtttt agataataga gaaaaaagag gaattagaag tattgaaatt gattcaaata
     1801 aaatcgttgc atatacatca aggctaaact ctatattatc ttgggataat aaatttatgc
     1861 tatatataat aaataaaaat tctaaggaaa ttgtatatga aggagaatta caatttaaca
     1921 gttcaaatag tatgttagaa tttgaaataa agtaggtgag ttgatgaaag atttaataag
     1981 attattcgaa tttgaattaa aaagaaatat aaagaattat atatttataa taatttgttg
     2041 ttgttctttt gtaattttaa acatagtaaa aaatttaaat gactataatt acataataga
     2101 aaatgcagtt aaaagtgaac agttaacaaa aatcggaaat acgatagaaa cggtaaatgt
     2161 tacaggtttc tcaagcttta gaaatataat gggttctaca gaatcgtggt ttatgtttgg
     2221 tataattgca tgtatttgct atgctttttt tatttggtat agagacttta atggtagaag
     2281 taaatctata tatacactta taatgctacc taaaaataga atcaatattt atatatctaa
     2341 attattaaat atattatttc tagtgtatag ctatacagta gtacttacaa tatctttgtt
     2401 tattgcaagt aagttgttgc ctagacatat gcttggtaat gtcacaaact atggttttgt
     2461 acaagaaaca atatatgagt taaaaatgtt acttccatat agctttgaaa tactatttgt
     2521 agagtatata tttttattga taggatttgt atctgtagta tttacatcaa ttcttataaa
     2581 taaatctatt tataaagtaa gcactttaat aagttttata tttttagtta tagaaatttt
     2641 agtgtttata atttctatag aatttattat accttacgaa tacagtgatg tatttactgt
     2701 tctatattca agtataaaca tatttgtatg tagtcttata tctaataaac ttttgaagca
     2761 aaagattgac ttttaggagg gatattatga aagcaaaaaa tataggaaaa tatacaatat
     2821 gttttataat agtatgtttt ttaatgatta ctatgacctt aatattgagt tattttaata
     2881 aggataaact atctgtaaaa actttaaaag gcaatgtaaa agatataggg agagtaagtg
     2941 tagtatatac acctaatata agcaaatttt cttatgagga ggtaattata agtaaaaatg
     3001 gagtaagaaa taatacaaac ccaaaatcaa gaattagaaa tgattattta aatattgaca
     3061 aaaaatacag taactttgtt aatgataaag gaaaagaatt tatatataaa gatgatttga
     3121 atataggaga agcgtatacc tctgttaagt ctgatttagt acaaggaatg aatgctttaa
     3181 atgattgtat aaaggtttca aataaaaatt taaaaagtgg ggtagaagaa aattttgata
     3241 ttaatttaaa taagtctgat tttaaagaaa atagttggaa aaccttattt aacaaaatat
     3301 ataaaggtaa tttatatatg gtaattagtg aaactaatat ttcaggttct aaagtcaata
     3361 atatttgtat attaaagata gatataaaaa ataagtctta taaattatta aatagattta
     3421 atttaggatt acagaatgga gaacaacaaa atatcaatga
//
LOCUS       DAEMHW010000282         3420 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEMHW010000282 DAEMHW010000000
VERSION     DAEMHW010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.284"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     repeat_region   470..1090
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=470..498
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            1737..3008
                     /locus_tag="KPK67_003611"
     CDS             1737..3008
                     /locus_tag="KPK67_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG2460526.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1337 a          431 c          401 g         1251 t
ORIGIN      
        1 ttaatctaaa attagctata ataacaatga aactatataa agcatataaa atttttttca
       61 taaaaaacac ctaaattcta tatttgtatt ttatattgag ccaaatctaa tttctgtata
      121 tttattaagt gtagttctag ttcctattgt agtagcatta aatatttttt aatttttatt
      181 ttcaaagggc atctccttat atttaaatta tagtatgaat attcataaat ttcaatctat
      241 ttataaggta attatttttt atatctaata tcttgtcatt aagttttatt atatatattt
      301 ataaattttt caatactttt tataattatt ctacttttat attttaatca ttaaaactaa
      361 agtcaatgtt atctggtaat tatttactac aaatgataaa aaataattat attttaaagg
      421 ataaaatgct gtataaatta ttggggtatt ttacctatac ttgttgcaaa tttacatacc
      481 acatagttaa tataaaacaa atttcaaaga gattggacaa cgaataaaaa ttgcaattta
      541 tataccatat agttaatata aagcatattt gtgtcgatat atagttctaa ctaagtataa
      601 gatttacata cctcatagtt aatataaaac aaaagcttat ttaaacgaaa tatacactaa
      661 aatagaattt acataccaca tagttaatat aaaacccaga aattcctcta aagctagata
      721 gtgctttacc gccatttaca taccacatag ttaatataaa actcttttct ataaggacat
      781 tttttacact tcttatctta tttacatacc acatagttaa tataaaaccc ttatctgtct
      841 tataagagta taaggtgcaa aaggatttac ataccacata gttaatataa aactatacct
      901 tttgctgctc ctgttattcc atcaactaaa tttacatacc acatagttaa tataaaactt
      961 gaatctaaaa gagtgtgaaa attgcgataa tatgagattt acataccaca tagttaatat
     1021 aaaactttat atcccttttc agaacttatg ttcgtaaaaa aatttacata ccacatagtt
     1081 aatataaaac cccaaaataa actaagtatt ttcagtactt atacacatac aactctctca
     1141 aatttgcagt gaaccatgag tagtgcaatt gataacattt atcatacacc ctcaatccct
     1201 tacattgcaa ctgttaaacc ctactttatc aaaaatatcg ctcactgcaa aatttatata
     1261 tttttattat atcataaaat tcaaactata aaattaaaat ggaataattt atcaatttaa
     1321 gaacacctac atatttgtaa atacttacta tattatctat tctaatagtt cttcttctag
     1381 ctagaactaa tataaaaatc atctctgaaa gattaggata tactaatata aaaataacta
     1441 taaatagata ttctcatatt ttaaacgata tggataaaaa agcttctgaa aatataatca
     1501 aagttctctt taaataacat tccattttat gtaagtaggc attatatttt tcttaaatgt
     1561 cacttaaatg tcagttatat ataataatag taaacaactg tatttcacta atatttgcta
     1621 ttatctaata gtatttatag tttagatttg atataataca tgtggtaaaa ttttgatttg
     1681 taaaacaaaa ttttacctac atatattaaa acaaatttaa aactaaggag acctatatgt
     1741 taaaagtatt aaaaggaaat ataattttta caaaaacttc agaaactttc acagtatttg
     1801 aaaatagtta tattatactg gaaaaaggta aagtaaaggg aatatttaaa gagataccta
     1861 aggaatacaa aaacctaaaa gtagtagatt acactgataa gcttataata cctggtatga
     1921 atgacctcca tgcccatgcc tctcaattta aaaatcttgg aatggctatg gataaagagc
     1981 ttttgccatg gcttgaaact tatacatttc cagaagaatc aaattataaa gatatagaat
     2041 atgcaacaaa aatgtataaa aaatttgtaa aagaactttg gaaatctgga actacaagaa
     2101 tagcagtgtt tgcaacagta cataaagaag cctctatgaa acttatggat atattcaaag
     2161 aagttggtct tggagcatac gttggtaaag taaatatgaa tatgaattgc cctgatactt
     2221 tattagaaaa cactcaggat tctattactg atacagaaga aattataaat aagtatagtg
     2281 atacagattc tattgtaaaa ccaataataa cacctagatt tataccaagc tgtacaagtg
     2341 aacttttaaa aggtttagga gatttatgtt taaaatataa tattcctatt caatcacact
     2401 tatctgaaaa ttatgatgag attgaatggg ttagaagtct agaacctgaa tctaaattct
     2461 atggagatgc atataataga tataatttat ttggtcaaac tcctacttta atggctcatt
     2521 gtgttcattg ttctcaggat gaaatagatt taatgagaga aaataatgtt atggcagttc
     2581 actgtccagc ttctaacttt aatgttggta gtggtgctat gcctatcaga aaattaatcg
     2641 ataataatat tagattagca cttggatcag atataagcgg tggacataca ttgtcaatat
     2701 ttaaagcaat ggtatctgca atacaattat ctaaattata ttgggttaat tctggtaaga
     2761 agtataattt cttatcttta tctgaggctt tttatattgc aaccaaaagt ggtggaagtt
     2821 tctttggaaa agttggtagc tttgaagaag gttatgattt tgatgcattg attattgatg
     2881 attcaaattt aaatcatgac aattattcta tacttgaaag acttgaaaga tttatctatg
     2941 ttggtgatga tagaaatatc attcatagat atgtatgtgg aaaactaatt gaagaaccta
     3001 atatataatt ataataaaaa ttttaattat atattatggt taatttatac aactataatt
     3061 aataaaagag tatattttaa atctaaaagc tataggttga aacatactct ttttattttg
     3121 tttatttata gctttagtta ttaatcatat ataaatctac aaaacgtatt cctagtaact
     3181 tatatttttt gatatctaca atcataagac tatattatta ttggattcag acctcaaaaa
     3241 tattgccttt tgaacattat tatattcaaa gctatgttaa tttacaatga tatatcctaa
     3301 catgtattga ttaaaaaaaa ttaaaacttg cttttcatct cctatatcta aagaagtggt
     3361 atttagaata aactattgta atattaaagt tgaaattttc tagtttattt actctatagc
//
LOCUS       DAEMHW010000283         3399 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEMHW010000283 DAEMHW010000000
VERSION     DAEMHW010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.285"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            184..549
                     /locus_tag="KPK67_003612"
     CDS             184..549
                     /locus_tag="KPK67_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2460527.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            572..2959
                     /gene="cadA"
                     /locus_tag="KPK67_003613"
     CDS             572..2959
                     /gene="cadA"
                     /locus_tag="KPK67_003613"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBG2460528.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
BASE COUNT         1354 a          329 c          628 g         1088 t
ORIGIN      
        1 cctagaaaac tataaaaaag tttacaaaaa cttattctaa taatatatat attaaaaaag
       61 tttataatat gtgcattcta tttatattaa attaataaaa attattgttg caaatttaga
      121 aatgataaca tataatgaac atatgaacag ttattcatat attaaaataa tgggggatta
      181 aaaatgagag aagagataaa tgattgtaat tgtaatatag tacatgaaga aatagtaaca
      241 gaggctaaat caactatgcc agatgaagaa atgttatatg atttagcaga attatttaaa
      301 gtatttgggg atactactag agtgaagata ttatatgcat tatttgcaaa tgaaatgtgt
      361 gtatgtgata tagcgagttt attaaatatg actcactctg caatatctca tcaacttaga
      421 gtattaaagc aggctagatt agtaaagttt agaagagaag gaaaaacagt gtattactca
      481 ttagatgata gtcatataag tcagatattt gattgtggat taaatcatat tagagagact
      541 tataaatgat aaatgagagg agtgaatata tatggaagca aataattcaa ttaaaaaaga
      601 atttatacta ggaggattaa attgtgctca ttgtgcagaa gagattaata ataaagtttc
      661 taaactacaa gaagttaaat cttctaattt aaattttata aataaaaaac ttacagtaaa
      721 tataaaagaa agctttaatg aggatactac tatagaaaag ataatagata taatagattc
      781 aacagagcca ggtcttgata tacaaataag ttctaaagaa aatgcagcta gtaaaacatc
      841 aattaaaaaa gagcttatat taggaggatt aaactgtgct cattgtgcag aagagattaa
      901 taacaaagtt tctaaattaa aagaagttga atcttctaat ttaaactttg taaataaaaa
      961 acttacagta aatataagta ataactttga agaggatgat gttataaata aaataaaaga
     1021 aataataaat tcaacagagc cagggcttga tatacaagta gggtctactg acaaagtaaa
     1081 agggagaact actgaaaaat caggagctgt aaatgataca aataagaaag aattaattcc
     1141 acttataatt ggagcacttg tatatatatt tggtatatat caaacagcta caggttatga
     1201 aagtcagttt agcaatatag tatttatagt tgcctatgta atagtaggtg gtgatgtgct
     1261 tttaagagca ataagaaata tatcaaaagg aagagtattt gatgagaact ttttgatggc
     1321 tttagcaaca gtaggagcat tagcaatagg ggagttatca gaagcagtag gggttatgct
     1381 tttctataaa gtaggagagt atttacaagg tgttgcagtt ggtaaatcca gaaagtctat
     1441 aacttcactt atgcaaataa gaccagacta tgcaaatcta aaagttaatt ctgaagttaa
     1501 ggtggtgtca ccagaagagg taaatgtagg tgatataata gtagtaaaac ctggtgaaaa
     1561 agtaccatta gatggtgtag tagtagatgg agtttctatg ttagatactt ctgcactcac
     1621 aggagagtca gtgctgagag aagttgaaaa aggagacgaa atcttatcag gtgttataaa
     1681 taaaaatgct ttattgagta ttgaagttac aaagagtttt ggagaatcaa cagtttctaa
     1741 gattcttgac cttgttgaaa attctagcat taaaaaatca aagacagaaa actttatatc
     1801 taagttttca agatactata ctccaatagt agtaatagct gctttattaa tagcatttgt
     1861 gccaccactt gtaatctcag gtgaggtatt tagtgattgg ttatatagag gattgatatt
     1921 cttagtcgtt tcttgccctt gtgcattagt tttatcaata ccacttagtt tctttagtgg
     1981 aattgggttt gcatctaaaa atggtatcct tattaaagga agtaactatt tagaagcttt
     2041 aagaagtgta gatacagtag tatttgataa aacaggaaca cttactaaag gtgtgttcaa
     2101 tgtaactaag ttaaatcctg aaggtatatc agaagaagaa ttattagaat atgcagctat
     2161 tgcggaggta aattcaaatc atccaatagc aaagtccata ttaagttact ataataaaaa
     2221 aattgattta gatacaatag acagttatga ggaaatagca gcttatggaa ttagagtaaa
     2281 gcataatggc aattttatat tagcaggtaa tgagaaactt atgaaaaaag aaaatatatc
     2341 ttattcatca gctaaagaag taggcacagt agtctatatt gctgttgata aagtatatag
     2401 aggttatata gttatctctg atgaagtaaa agaagatagt aaaaatgcca taagaagctt
     2461 aaaagaaata ggggtaaaag aagttgtaat gttaactgga gataatgaga aagtagcaaa
     2521 aaatattgct caagaattag aattggatac agtttactca aatttacttc ctaatgaaaa
     2581 ggtagataga ttagaagatt tatatgaagg aagaactgaa aaagagaaaa tagcatttgt
     2641 aggtgatgga ataaatgatg ctccagtatt agctcgtgct gatgttggta tagctatggg
     2701 aggcttaggt tcagatgctg ctattgaagc tgcagatgta gtgcttatga cagatgagcc
     2761 tagcaagata tctaaagcaa ttgaaatagc aaacaagact aataagatag tgtggcaaaa
     2821 cataatattt gctctaggag taaaaattat agttatgata ctaggtgctg gaggagtggc
     2881 tactatgtgg gaagctatat ttgctgatgt aggtgtggca cttatagcag tagtaaatgc
     2941 tatgagagct atgagataag ggataaatgt aaatccatac tatttaaaaa acttataatt
     3001 atatattgaa gtatgtaata atgaaaggta tcaataagtt tcttttaact ttattggtac
     3061 ctttttattg gagcgctttg catatataac tttatattca ttatccatta tgtttattta
     3121 atgtatttat ttagtgtaga gcttttatat tgtaatttta aaatttagac atagaaggtt
     3181 tattaataag tttatattaa gtttaaatta gattcataaa aattataata cttatgattg
     3241 tatatatatt atattaatag ttgtaatttt tacaacaaaa tgtgtgtgtt aaaatactct
     3301 aaaccaacag tatgactgcc attcaataaa ttatgtaata taagaaagta aatattttta
     3361 aaaaataatt taaaaaagta ttgacgaatt atttttaag
//
LOCUS       DAEMHW010000284         3399 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEMHW010000284 DAEMHW010000000
VERSION     DAEMHW010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.286"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(246..449)
                     /locus_tag="KPK67_003614"
     CDS             complement(246..449)
                     /locus_tag="KPK67_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG2460529.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            740..1504
                     /locus_tag="KPK67_003615"
     CDS             740..1504
                     /locus_tag="KPK67_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG2460530.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            1893..3206
                     /gene="prdC"
                     /locus_tag="KPK67_003616"
     CDS             1893..3206
                     /gene="prdC"
                     /locus_tag="KPK67_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBG2460531.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
BASE COUNT         1377 a          363 c          586 g         1073 t
ORIGIN      
        1 tatataaaat taaaagtttg tgataggcta attcttcaga cgagttttca aacataaata
       61 tcatcctccg tcaaactatt tctatatata atatattaaa ctaatttgta caaaattaaa
      121 agaacaattt gtattgtatt attaatcttt aataattcat ataaaaacaa ttttataaat
      181 aaatatagaa aaacagggct gctaattagc agccctgttg atacccaggt gtcaaaattt
      241 actatttatt ttccagccat ttgtctttca gccatttcaa ctaatttctt agtcatatat
      301 ccaccaacat atccattttc tctagctgtt aagttacctt tgtctatgtt ttcatagtta
      361 gataaaccta tttcgttagc tatttctaat ttcatttggt ttaaagcagc ttttgcttct
      421 ggaactactg ttctgtttga atttgacata gtaaatttcc tcctcaaata attagtttat
      481 gatgtctaac aacactttta tttatgtgat gttaatacta atttctccaa aataaaaata
      541 atatatacag aaaaataaag gtaaaaatgt aaaaaatata aaaaaattaa ataaatgagt
      601 attttagaga gtttaattag gtcaatatag gttttatttt tttagttatt gtcttataaa
      661 ttaaatatta cagtactttt ttgagctgaa aaaaaataaa ttttaaatat agaagaaatt
      721 atttcaaagg gggaatagta tgaaaagaga agttaaaagg actaaagctt tgatatatag
      781 tgttttattt ttttcattat tattagtgtt aatatttgtt tttgtttatg gaataaaata
      841 catatgttct aaatctgaca attcaaaaat gcctatatat aaagttgata caaatgagaa
      901 aaaaatagct ttgagttttg atgtagcatg gggaactgat aatatggatg atattttaaa
      961 aattttagat aaacataatg taaaagctac cttcttttta gttggtagtt gggtggatga
     1021 taatgaagaa attgtaaaag ctatagatga aaaaggacat gaaattggaa atcattcaaa
     1081 tactcatgcc aatttgaagg aaatatcaaa agaagacata acaaaagaga tagaaactac
     1141 atcagaaaaa atatataata taacaggaaa aaggactaat ttatttagac ctccttttgg
     1201 ggacgtaaac aataaagcta tggacatttg tagtgattta ggttataaag taattaagtg
     1261 ggatgtagat tctatagact ggaaggagct tggaccaaat catgtaattg aaaaagtaat
     1321 aaaagaatct caacctggtt caatagtttt attccatgct aatataaatg atgtagataa
     1381 ctatttggat actataataa gtcgactaaa aaaagatgga tacagtctag tcaaaatatc
     1441 tgatttgttg tataaagata attatgtagt tgattccaat ggagtgcaaa aaactaagaa
     1501 ttaataagta ataaatgtat attttaagta aagttgtgat aaattatata gtatatgtta
     1561 gaaaagtgtc gataaaaaga cggtgtctat tttttgacac tttttataca tgtatgcaat
     1621 atcaataatt atagattaaa ttgattaaaa aagtgtctaa taattgactt ttttttgatg
     1681 ggtttaaaaa tttagaagaa ttaatgaatg ctgtaagaca gtatttccaa agggtataag
     1741 tttgtttaaa agttggcata gaaattgctt tgttaataag cgatatgttc tatagaaata
     1801 aacaatatgt taagacctta taatagaaaa atagcattaa cattatttga acagcttata
     1861 aaaaaattaa tatacttaag ggggcgtact agttgaacaa actatattct attttattaa
     1921 aacaacacgt tggtggtcca gataagccag tagtaagtgt tggagatgta gtaaaaaaag
     1981 gaactctaat cgcagaacca actggattag gggcaaatat ttatgccagt gttagtggaa
     2041 aaataagtga aataaatgac caagcgatag taattgaagc tgatgaagta caagaagata
     2101 cttttgaacc attaaaagga gaaggtatac ttgacttaat taaagaagct ggagtagtag
     2161 gaatgggtgg agcaggattc cctactcatg taaaattaaa catagattta aatggtggaa
     2221 caatattagc aaatgcagct gaatgtgaac ctttattagc tcataatata aaagaaatag
     2281 aagaaagacc agaaatagtt tatcaaggta taaaatatgc tatggaagtt actaatgctg
     2341 gaaaaggtat gctagcaata aaaagtaagc atccaaaagc aatagaagct ttcaaaaaag
     2401 taataaagcc aggagacaac atagaagttg cagaactagt tgatatgtac ccaatgggag
     2461 aagagagagc aatagtaaga gacgttctag gaaaattact tgagccaact caattacctt
     2521 cagaggctaa tgcagtagta ataaacgtag aaacactaac tagaatagta gaggctgttg
     2581 agcaaaaaag accagttata tctaaaaata taactgtagt tggtcaatta aacagtggaa
     2641 aagaatctat agtgtttgaa gatgtgccaa ttggaacaac tgtcggagaa ttaattgaaa
     2701 gagctggtgg aataaaaggt gaatatggtg aaataatcct aggaggacct ttcactggta
     2761 aagctactac tttagatgct ccaataacta aaactagtgg tggtataata gttactatgc
     2821 catttgttaa tgaaaagaga aaaatgggtc ttttagtttg tgcatgtgga cctaatgaag
     2881 aaagaatgag agatatagca actaaaatgg gagttactga tatagtatca gttcaaaaat
     2941 gtaaacaagc tcaagaaatt aaaggtgcat taaaatgtga aaatccagga cactgtccag
     3001 gacaagctca aaaatgtatt gaatttaaga aagctggagc agaagttata ttaataggaa
     3061 actgtactga ctgtagtaac acagtaatgg gttctgcacc aaaattaaaa ttaggtgtat
     3121 atcatattac tgaccatgta atgagaacag ttaatcatcc attaataaga agaataaaag
     3181 gtgacgtaag attaaaaata aaataataga taaatagaag ttagacatct ttagaagtaa
     3241 aacttaaaag ttacttctaa agatgttttt taatgtattt tgagggcaat atatattgta
     3301 aaataaatgg aaaaaataga caaaatatgc tagatattgt ataatgataa caatagctat
     3361 tacaattaat attggacaaa aataatgata ctcaagtat
//
LOCUS       DAEMHW010000285         3388 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEMHW010000285 DAEMHW010000000
VERSION     DAEMHW010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.287"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            42..1478
                     /locus_tag="KPK67_003617"
     CDS             42..1478
                     /locus_tag="KPK67_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBG2460532.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
     gene            1726..3162
                     /gene="miaB"
                     /locus_tag="KPK67_003618"
     CDS             1726..3162
                     /gene="miaB"
                     /locus_tag="KPK67_003618"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBG2460533.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT         1406 a          339 c          583 g         1060 t
ORIGIN      
        1 tttatatatt aaaaatttat tatgaggggg acaattttat tatgaaattc tataaaagaa
       61 tattaacatt gactttaagt atttcttttg tatttgcaaa tattcaatta gtagatgcaa
      121 tatcatctgt agaaaaaata caaggaaaaa ataagtatga aatagcaggt aaaatagcag
      181 ataaaaatgc atataaaact gctatattaa ttaatactag taactctatt gcagatggat
      241 taagtgcaag tggactagca ggagctttaa atgcccctat actattaact gaaaaaaata
      301 ctataccaac tgagacatct gcaagattaa aaaatgtatc aaaagtttat ataattggag
      361 gaacatattc tattagtaca tctgtcgaaa attcattaaa atctaagaaa atgaaagttg
      421 taagaattaa gggaaatgac agaataaaaa caagttataa tgttgcaaaa gaaataaatt
      481 caataaaaaa agtcaatact gttatgctaa ccaatgcata taaaggagaa gcagatgcta
      541 taagtattgc atctgtagca gcaagagata aagcaccaat aattcttact aatggacaaa
      601 gtataccttt ttcaactagt ggtttaaagt cttatgtaat aggaggaaca gcttcaatga
      661 gtaccacttt agtaaacaat actaaatcta ctagactagg ggggagtact cgttttgaaa
      721 ctaataaagc aataataaat aagttttata aggatgctag agagttttat atagctggag
      781 cttatgaatt aacaaatgca cttgtaggat cttctttatc aaaacatggt ccaatggttt
      841 tagttaatga tggtagcaat aaaagcattt taaaaaatgc taagaagata acttctatag
      901 gatatataga ttcaaatata gtacaacagt gtttaaatat aacaaatgga attggagata
      961 ttaatactgg ggttgtaaaa aatgttaaac ctactacaaa gactataaaa gatggaatgt
     1021 ataaggttgg aaaagatata tcagcaggtg aatacttaat aacttcaaac tcaggaagtt
     1081 atgcttctta ttatgaagtt acaagtgata gtacaggtaa tgctgatagt atactttcaa
     1141 atgatatttt tagtgggact agatatatca ccttaaagaa tggtcaatat attaaaattg
     1201 aggattcaac aatgacatta gcaaagtatg ctaaagccca gaaggccaag aatggtaagt
     1261 ttggaaatgg tatgtataaa attggattag aaattcctgc tggagaatat attataatgt
     1321 caaattcttc tgatgcatat tatgaagtta gaaatgatag cttaggtaat gcagagggaa
     1381 tagttactaa tgatactttt tcaggaagaa gatatataac ggttgaggaa ggtcaatatt
     1441 taatattaaa tgattgttat ttaattgaaa atgaatagat gtaattgtat ttaatagtaa
     1501 atcatatatt ttatttaagg aaagatgttt taagttttaa tttaaaacat ctttttatta
     1561 ttataataat tatatgattt tatattttag aaatatagta tatataaatc attagattta
     1621 ctattaaaac acaactagaa aaataataat atctttaaaa gatttttctt atttgatata
     1681 attaaggtta tgtgtaatat aagaaattaa ctaggaggaa tagacgtgag caaaagaaaa
     1741 caagtaacaa tacctataga gaaaatacaa gaacaagata aatatataga acaaatacac
     1801 agacaaaatg aagaatactt caacagaact ggaaagagaa aactcgtgtt tactcaaacc
     1861 ttcgggtgtc aaatgaatga acatgattca gaaaaattat gttcaatgtt agaagagatg
     1921 ggataccaaa tgtcaatgat ggttgaagaa agtgatttaa taatatataa cacctgtgca
     1981 gttagagaaa atgcagagct aaaagtgtat ggtaacttag gacaattaaa acatctaaaa
     2041 ggaaaaaatc cagatatgaa aatagctgta tgtggatgta tgatgcaaca accacatgtt
     2101 gtagaggaat taagaaaaaa atataaacat gtagacctta tatttggaac acataatcta
     2161 tataaatttc cgcaattact tacagaatca ataaattctg acaaaatgtt agtggatgta
     2221 tgggatgtgg atggagaagt aatagaaggt cttagaagta atagaaaatt tgaattaaaa
     2281 gcatttgtaa atataatgta tggatgcaat aatttctgca catattgtat agtcccatac
     2341 acaagaggaa gagaaagaag tagaactcct gaagatataa ttaacgagat aaaagagctt
     2401 gttgcaaatg gaacgaaaga aatcacttta cttggacaaa atgtagattc ttatggtaag
     2461 acacttgaaa atccagtttc attctcagaa cttttaagaa aagtgaatga tatagaagga
     2521 attgaaaggg ttcgatttat gacatctcat ccaaaagata tatctgatga agtaatatac
     2581 gcaattagag attgtgataa ggtgtgtgag tttttacatc taccaataca atgtggaagc
     2641 agtagccttt tgaaaaaaat gaatagacat tatacaaagg aatattattt agaaataata
     2701 gaaaaagcta aaaaggaagt tccaggtatt gctttttcta cagacttaat gattggtttc
     2761 ccaggagaaa cagaagaaga tttattggac actttagatg tggttgaaaa agttagatat
     2821 gattcagctt ttacatttat ttattctaaa agacagggaa ctcctgctgc taaaatggaa
     2881 aatcaaatac cagaagatat aaagcatgat agatttaata gggtgttaga agctgtaaat
     2941 aggatttctg cagaaataaa tgatggatat aaagatagaa tagttgaagt tctagttgaa
     3001 ggaagaagta aaaataatga aaataagttt gctggtagaa ctagacaaaa taaactcgta
     3061 aattttgaag gtggaaatga tgatttaatt ggtaaattag ttatggtcaa aattactgaa
     3121 cctagaactt tttcattaaa tgggatatta gtaaataatt aatattgtac aaaataataa
     3181 aattatgtaa actaatcaaa atagatgtac tatagaacaa attatattaa aaatttttat
     3241 tttaagcgaa tttgtcatat gttatgctaa tgtaagtttt tgctattaat aaaaataaat
     3301 ttgatataag atataatttc attgtacatc tatttttgtt ttattatatt ttataaaagt
     3361 ttacttagtt gtcattagta attccctg
//
LOCUS       DAEMHW010000286         3372 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEMHW010000286 DAEMHW010000000
VERSION     DAEMHW010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.288"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            99..779
                     /locus_tag="KPK67_003619"
     CDS             99..779
                     /locus_tag="KPK67_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG2460534.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKKLTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            772..2151
                     /locus_tag="KPK67_003620"
     CDS             772..2151
                     /locus_tag="KPK67_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG2460535.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKTSMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            2322..>3372
                     /locus_tag="KPK67_003621"
     CDS             2322..>3372
                     /locus_tag="KPK67_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG2460536.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAM"
BASE COUNT         1456 a          303 c          559 g         1054 t
ORIGIN      
        1 ttaatatata actttgatat aataagcata tgtaaatgtt taatatttaa ttaaagaaaa
       61 ttatcatata aaaataaatt tgtaaggagc aatacagtat gaaaaaacaa tatgtgttta
      121 taatactagc aagtactatt ttaggtatac ttgttggaac catatttaga gattatagca
      181 atgaagaaac agtttataca agcaaagaca agtcaataag aaaagaaata aaaatgacaa
      241 gaaaatctat aaaaaaatta accaaagaga aagacaagtt agataaagaa attaattcaa
      301 tgaaacaaaa tcaaaaaaat tctgaagaaa tatcagaaat agaaacactt agagatagtc
      361 tatcatattc agatgtaaag ggtagtggga taatattaag tatagatgct atagatgaaa
      421 aaacagggaa tatagctaat agtatagact ataataagat tcttgtaaaa gttataaatg
      481 aattaaaagt aaacggagga aaatacatag aaataaatgg tcaaagaatt aatcaatatt
      541 cagaagttgt acttgcagga agccatataa atataaatgg agttcctgta gcacaaccat
      601 atgaaataaa gtgcattgga gataaggaga aactatctaa ttatgtagat aaaggtagta
      661 aatatttaga tagcatacaa accaattatc caatgaaaat aagcataaaa actgaagaaa
      721 atatctctat gaaaaaaatg aatataccta ataaattaaa gtatataaag ggtgattaag
      781 attatattag aggaaagcag tttgccaatg ttaataccag aaaaacttgc aaaaaaatat
      841 aatgtatttc cagttgagtt tcaagatgat aatttagttg tagaaataga agatgaggat
      901 atttatgctc tacaggactt gagattagct acaggtaaag aaattatatt gaagaaggag
      961 aaaaaagaac ttataagtga aaagattgaa aaatattata atagctttga acttgatgag
     1021 gattatgcaa aaaaattatt tgaaaatctt ttagaaaaag ctataaaaga aaacgcaagt
     1081 gatatacata tagaaccatt taaagaacat ttaatagtga gaatgagggt agatggagaa
     1141 ctaaaggaag tatcaaagtt tttagttgat gtctatccat cattgtctac ggttataaaa
     1201 ttaaagacat caatggatat aacagaaaaa aggcttcctc aggatggtag ggtggatata
     1261 aagttaggtg gaaatttaat tgacattaga gtttcatcaa taccaactgt ctatggtgaa
     1321 aaaatagttt tgaggatact taatagagat acttttttaa aagacaaatt agaacttggt
     1381 ttttcagagg aagctataaa atctataaat cgtataataa ataaaagagc aggaatatta
     1441 ttagtgacag gtcctacagg tagtggaaag actacaacag tatattcctt attaaatgat
     1501 ttaaaaggaa taaataaaaa tattatgact atagaaaatc ctgtagagta taagatggat
     1561 ggcataagtc agattcaagt aaattcaaaa gtagggttga gctttgatgt aggtcttaga
     1621 gcgatattaa gacaagaccc agatataata atggtcggag aaataagaga tgcagaaaca
     1681 gctaagatag cagtaagagc agctataaca ggtcatttag ttataagtac tttacatact
     1741 aatgatgcag tatcttccat agctagatta ctagaaatgc aaataccacc atatctttta
     1801 aatgcctctc ttataggtgt tatatctcaa aagttggtta gaaaagtctg caatcattgt
     1861 agccatgaga ttatgataaa agataatttt agtggagatg taaatactaa ggtagctgta
     1921 gggtgtgaaa aatgtaatga taaaggatac tttggaagaa cagcaattta tgaaatctta
     1981 gaaattaatg atgatataaa gacatgtata agaaatatgg aggattctag tactattaaa
     2041 gatgtggcac aaaataatgg aatgataaca tttgaagata gttgtaaaag actaataaat
     2101 gaaaaaataa cgacattaga agagtgttta gtagtaaatg aaatgattta actgaattgt
     2161 atagttaaac ttcgtttggt cagttgtctt tatttaatct attttaatta agatgtttga
     2221 gtataaatta tatttaagtg aaataaaatt aaatgcatta aattagaaaa tattaagttt
     2281 atttaattgt aattaatata atttggaagg tattatagta catgagatta tctaaaaata
     2341 tattttataa tttagaaaag ttttatttag tattaaatag aatcagtgat aaagaaatta
     2401 gagtaatttg caaagagata ggcatattgc tggaatctgg atgtgaaatt acaaaaatat
     2461 ttgaaatcat tgagagtcag tcaagtaaaa aagtaaagaa tttgcttagt atagtatcaa
     2521 atcatattca aaaaggtaac tcaatagcag aatcatttca aataacaggt atattttcta
     2581 aattctttat aagtatgata aaagcaggag aaactagtgg aaacttagat ataataatga
     2641 gtgatttatc taattattat gataaagaat acaaattaaa aatgaaaata ataactattt
     2701 caatatatcc aataatactt ataatattat ctatcttatc aatgttgttt atattcgtat
     2761 ttgtaatacc taattttcag gtggttttta ccaacaatgg aattgaacca cctttaataa
     2821 ctagagtttt aatgggaata tctacagtgg ttacaaataa cttagcttat ataatattta
     2881 gttttatatt gttttcaata ggaagcattt atttctttat aacaaatgat aatataaaaa
     2941 aattgattaa ttcgttaaaa ttcaaaatac cttttataaa aaaaattaat cagttagtag
     3001 ctacaacaag attttcaagg gcattgtccg ttttaattag cagtggaata caaattgtgg
     3061 aagctataga catgtcagct agtgttatag acaacgaatt tatttatgaa aggttaaaga
     3121 tttccaataa ttatataaaa aaaggaaata gcataggtaa atctttgaat ttagcaaatg
     3181 tttttccaaa attatttatt tcaatggcaa atacaggaga agaaagtggg aaactagata
     3241 aaagtctaaa tgtaataaat aaattttatg aaaatgaatt aaataacaaa atagagcaaa
     3301 taatgaaatt tatagaacct atgctaattg tatttatggg attaattatt ggtactttta
     3361 ttttagcaat gg
//
LOCUS       DAEMHW010000287         3353 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEMHW010000287 DAEMHW010000000
VERSION     DAEMHW010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.289"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(142..966)
                     /gene="spo0A"
                     /locus_tag="KPK67_003622"
     CDS             complement(142..966)
                     /gene="spo0A"
                     /locus_tag="KPK67_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBG2460537.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            complement(1108..2166)
                     /locus_tag="KPK67_003623"
     CDS             complement(1108..2166)
                     /locus_tag="KPK67_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG2460538.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            2327..2767
                     /locus_tag="KPK67_003624"
     CDS             2327..2767
                     /locus_tag="KPK67_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2460539.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIEFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            2828..3319
                     /locus_tag="KPK67_003625"
     CDS             2828..3319
                     /locus_tag="KPK67_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2460540.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKIGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
BASE COUNT         1093 a          519 c          395 g         1346 t
ORIGIN      
        1 agtaagcaaa taataaagaa aatttttata tttattaaaa tagcgaaacg gtataaccct
       61 agtggttata ccgtttcgct gcttgtaata cgcatttata gccaatgcct taattaaaaa
      121 gccttacttt ttatgtcttg tttatttaac catactatgt tctagtctta atttatcagc
      181 aatcattgct ataaattctg aattagttgg ttttccttta gtattgtgta ccgtatatcc
      241 aaataattga tttattgtgt caacttttcc tctactccat gcaacctcta ttgcatgtct
      301 tattgctctt tcaactctac ttggtgttgt attgaatttt ttagctatac ttggatataa
      361 ctcttttgtt actgcaccta aaagctcaac attatcaata accattttta ttgcttctct
      421 taaatataaa tatcctttta tatgagctgg tactcctatt tcatgtatta tatttgtgat
      481 ttctgtctct atatttccta cattcttaac aaaatcactt ctagtcattt gagtctcttg
      541 aactggtcta ggttttggct caacttgtgt aactctatta gaaactagct ctctaattct
      601 gtttataaac acaacaaaat caaatggctt tactatgtag taatctgctc ctagatttat
      661 tgcgctttga gttatcttat cttgacctac tgctgatagt actattattt ttggcatttt
      721 aggtatatcc atagtattca atttttcaat tacacctaat ccatctagat gtggcattat
      781 tacatctagt attaataagt ccggttgtgt cttttttact aagtctaatg cttcaattcc
      841 atccttagct atgcctaata tatcgatatc atcttcatta gacaaatact cttttaatac
      901 ctgacaaaaa tccttattgt catctgctaa aactattttg attttttcca ctaaaaatcc
      961 ccccattaaa aaacatcttc ttattacaga aaactaaact ttttacacaa ttgcttccta
     1021 attatctact ctaaagcata ttcgacactc caaaattata ttcctcctag ataattgata
     1081 aaataataaa attttttaat gcctcaccta caattttatc atttcatcta taaaaatacc
     1141 atatcctttt ttcggattat ctctaaaaac atgagttatt gcaccaataa ttttattatt
     1201 ttgtattatt ggagctccac tcatcccctg tacgatacct cctgtgtatt ccaataactt
     1261 gtcatctact acttttatta ccatatctct ataatttcct ttatcatata ctatactttc
     1321 tatttttata tcatagcttg ttatattttt gtttttatcc tcaaaaagaa ttactgcatc
     1381 tcctattttt acatcttgcg gtcttccaac ttctattaat tgcttttctt tatttgctac
     1441 attaaactct ttattatggt tttctgtaat attaccactt atacctaact ctgaattatt
     1501 tgagaaatta cccattaaat tgctgtcatt aaaatctcct tttattttac ctactttttc
     1561 attactagct tttactattt ttaattgttc tggcttatat atatatcctt gctttatctt
     1621 taatagttca ttagtatccg aatcttttat ggcatgaccg attgctttga acttttccat
     1681 actagggtcg taaaaagtca tagttccaat tccagatatt ttatctctta cccataatcc
     1741 aagtttatac ttgccatttt cttttttagt ctctattact tctgttttat acttattttt
     1801 tctctcaaaa gtcacttcaa ctttactttt tttaatctta ttcaaaatct ctgatacatc
     1861 ttgactattt tttatccttt tattatctat ttttactata ttatcaccga tttgtatgcc
     1921 tccaatatag tctacatcct cttcctcata tcctaagacc aatacaccat ctgtatttgc
     1981 ttttactcca attatatttc ccataggata tacaaatttt ttatcatgat tcttaattgt
     2041 aaaagcttct atttgagtct ctttattaac attacaattt ttattaaaaa ttataaccat
     2101 ggtaaaaatc gatacagctc ctaatattat ccaaatattt ttgaaatttt tatttatttt
     2161 gttcatatat ccatctactc ctatgctgta ttattatgta tttataattc ctttttcctg
     2221 agaatttatt catttaaaaa gtttatttaa aatcttaata agtgattttt aatacataat
     2281 atgatagtat tagattgtaa caaaaagttt gaagggcggt aatatcttga aattaaatgt
     2341 gagaattgca gacataaata attggcatga tttagttagt ttgtctgtag ataaaagtca
     2401 gttagattat atagaaagca atgcactttc tattgctgaa tctaaattta taacagcttg
     2461 ggtacctgtt ggaatatatg atgaaaattc tttaatagaa tttgctatgt atggtcgttt
     2521 agaagatgat agagtttggc ttgatagatt tatgattgat tcaaaatatc aaggtaaagg
     2581 atatggaaaa gcatcattag attttcttgt aaatcatctt aaaaatgaat ataactgtga
     2641 tgaactatat cttagtatat tcgaagacaa taaaatggct attaagcttt ataaagattt
     2701 tggatttgag tttaatggag agcttgacta tggtggtgaa aaagtaatgg tattaaaatc
     2761 taattgatat taagaaaaat atacatatat caaggaggtg aagtcattat ataaacatgt
     2821 ttatataatg gcggataaaa tggctcaaaa tataacttta caatttgttg aagaaaaaga
     2881 tttagaaaac cttgaaaacg taaaacttct atttactgaa tattcaaatt ctctaaatat
     2941 agacttatgt tttcaagact ttaataatga attaaaaaca cttccaggaa aatataaaaa
     3001 accttcaggc tcattgatat tggcatttgt tgatgaaaat ttagcaggtt gtgttgcttt
     3061 aaaaaaactt gaaggtaaaa tatgtgaatt aaaaagatta tatgtaagaa atcaatttag
     3121 aggattaaaa ataggaaaaa tactattgga agaaatcatt gaagaagcta aaaaaattgg
     3181 atatacacac atgagattag atactcttcc aagcatgaaa agtgcacaag gattatatga
     3241 aaagtttggt ttttatgata tagaacctta tacttataat ccaattgaag gggctagata
     3301 tatggaatta aaattataat tttttaatag agtataagta aaactactac agt
//
LOCUS       DAEMHW010000288         3295 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEMHW010000288 DAEMHW010000000
VERSION     DAEMHW010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.290"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            112..774
                     /locus_tag="KPK67_003626"
     CDS             112..774
                     /locus_tag="KPK67_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460541.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            1145..2554
                     /locus_tag="KPK67_003627"
     CDS             1145..2554
                     /locus_tag="KPK67_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBG2460542.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            2669..2944
                     /locus_tag="KPK67_003628"
     CDS             2669..2944
                     /locus_tag="KPK67_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBG2460543.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
BASE COUNT         1367 a          313 c          580 g         1035 t
ORIGIN      
        1 taaaaataaa tttattttgc taataaacat gttattaaca aaagaatagt actataatgt
       61 atagatgata aaaactttaa taaaaattta gtgtatagga gttgtttaag tatgatagat
      121 gtaagtattg atgacaaact taaggaaaga tggcctaatg ctaagctggg ttgcattcaa
      181 gcaaaagtag cagtaaataa aagctcagaa aaattaatta atgaaataaa tgagttttgt
      241 gatactataa atcaaagtct aaaagttgaa gacattacga aactagataa aattaaagat
      301 gcaagaaatg cttataaaga gcttggaaaa aatccaagta gatatagaac atcttcggaa
      361 gcattggtta gaagaattat tcaagggaag ggtctttata caataaataa tatagtagaa
      421 ataaacaatt taatatcaat aaaatcatta tatccagtag gtacatataa tgtaagcaaa
      481 ttacattcac cagtatgttt tactgtagga gatgaaggag aacagtataa gggtattggg
      541 aaagaattaa taaatataga aaatcttcct atattgtctg attcagtggg taaatttggg
      601 agtccaacta gtgattctga aagagcaatg ataacagaag atgcaaatga aatattaatt
      661 tgtatatttt cttttagtca agaatctgat attgaagaat attttggata tgctaaggaa
      721 ttacttaaag aatatgcaga tggaaaagat atagaaacta aaataattaa atagaatcta
      781 tataacaacg ttatgttaag ctaaaattca atataaaaat aagttaagat aaagaaacta
      841 acacaaaatt agtttctttt ttaaaagagt agtttagtgt tacaaaaata gatagatagt
      901 cccacgatta ttaaaagtat ggtaatatat taatatagca gagattgtgt ttaagggatt
      961 ttgaaagggg ggatttataa taatcatttt atctaaaaga taaaatggtt ttatggaaat
     1021 aactatatat ttaaaaatat aaataatgat acagtaattt gggtatataa aataaggcat
     1081 acatggtttt gtttaaaaac caataaggta cataggtaat tacaatgtaa ggaaggattt
     1141 aattatgatt gatggaaaag aggtaaaaca agaaagtgga caaaaaaatc taaaatttaa
     1201 gatagctata gtagtaggtg ttttgtttgt agcttatata ggaatggcaa tcttttttag
     1261 aaatcatttt tactttggaa cgactattaa tggcgtaaag gcttcaggaa aaacagttga
     1321 gcaagtaaat gatttgttaa catctagtac taattcctat actttaaact tagaggaaag
     1381 aaacaataag aaagaacaaa taaaagcaaa agatattgat ttaaaattta caccagatga
     1441 taaagttcaa aagataaaag atagtcagag cgctattgga tggattggtg gaatatttaa
     1501 atctaaagag tataataata tgataactct ttcatataat aaagatttac tagaaaaaag
     1561 gtttaatgct ttatcttgtt ttgatagtaa aaatgttgta gaacctaaga gtgcttatcc
     1621 taagtatgat agcactaaaa acgcttatgt tatagtacca gaggtacttg gaaacaaagt
     1681 gaaaaaagat gatttttata agttagtaca aaactcaatt aattctggga atacttctat
     1741 ttctctggat gaaagtaaag tttatgaagc accaaacaac acaaaggatt cagaaaagtt
     1801 gaaacaagct attaagacat tagataagta tgttggtgta gtcgttacat atgattttag
     1861 tgaccgtaag gaaactttag atggttctga aattaataaa tggttaaaag tagactcaga
     1921 aaagaattat gacataactt ttgataatga tgctatggta gaatatacaa gaggtttaag
     1981 taaaaaatac agtacttttg gtgactcaag accatttaaa actgccacag gtaaagatat
     2041 tacaatttca ggtggaattt atggttggtt gatagataaa aagaaagaag ctgaagcatt
     2101 agttgatgtt gtaaaagctg gtaaaaatgt tacaagagaa cctatttaca tacaaaaagc
     2161 tgtatctcgt aataaagatg atattggaaa tacatatgta gagattagtc tttctggcca
     2221 acatatgtgg ttctttaaaa atggagaaat gcttgtatca acagacgttg taacaggtga
     2281 tttagctaga ggatttgcta caccatcagg tatatatcct cttaattata aggctagaaa
     2341 tgtttcatta actggtcaag gttacagttc acctgtaagt tattggcttc catttaatgg
     2401 gaacataggc attcatgatg ctacttggag aaattctttt ggtggaagta tatataagtc
     2461 ttcaggttca catggatgtg taaatacacc tttttctaag gcaaagaaaa tatatgaaaa
     2521 tatagaacca ggtacaccaa ttattttata ctagtaaatg tgttgctaac agttaagcga
     2581 agtcatctta gtttatttag gatggcttcc tttttatata aagcttattg aagtttacag
     2641 aaagactact taaaaggaga aatacataat gaatgaaaaa ttaactaaaa ttttagaaga
     2701 agctaagaat gatgatgaat ttagaaagga acttataaaa acaagagaac aacgtgatgt
     2761 tatggattca ttttgtaagg ttgttacatc tagaggttat gatatgacag taggggaact
     2821 tttttctatg ggagaagaat atacatccaa tctactgaaa agtgtaaatg gaggagcagc
     2881 atatcctatt gaagattgga gtgattggta cgaggatttt tttataatgt tgtcgaatat
     2941 atagagtaac taatgatatt atctacttaa gattaataca ttgccttaaa ataaaactat
     3001 tagaactagg tgtaagtttt atcaatcttt aaaaatattg atttatttct aactagatat
     3061 aaaaaaatta gattaaaaat aaggtgacaa aaatcgatat agcttgatga tttataaaag
     3121 ttagataaaa tagtgcgaga tatttctaaa aattcaaaaa aaacaataaa cttcaagggt
     3181 tagaggttga aaaagaatat aaaaaacttt tatatgtaat aaattaaatt aaaattataa
     3241 aaaaaataaa ataaaaattt aaaatgtcga cataacgtta attttaaaac aaaat
//
LOCUS       DAEMHW010000289         3274 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEMHW010000289 DAEMHW010000000
VERSION     DAEMHW010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.291"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            242..1510
                     /gene="eam"
                     /locus_tag="KPK67_003629"
     CDS             242..1510
                     /gene="eam"
                     /locus_tag="KPK67_003629"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBG2460544.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(1603..1959)
                     /locus_tag="KPK67_003630"
     CDS             complement(1603..1959)
                     /locus_tag="KPK67_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460545.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(2110..3204)
                     /locus_tag="KPK67_003631"
     CDS             complement(2110..3204)
                     /locus_tag="KPK67_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG2460546.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         1339 a          429 c          469 g         1037 t
ORIGIN      
        1 aaaatagaaa aaattaatca tttgttaaaa aaaatgcata atttattgta atttattgac
       61 gaaggttgaa aaatattcat aaatgtgcga aataacagaa aaaatgcaaa aattaattca
      121 atttcatttg ttataaaatc tatatggcaa aattaaatct gcctatcaga atgatataaa
      181 cacatcagaa aggaggacgc tatcaagtaa tacaagtagt taactttgat agcaaaaaat
      241 tatgaatgaa caaactagaa tatccttaga gagagctgct gaattaaaat caaaaattga
      301 tgattatatt caggctagaa aaacgattaa cagaggtctt gaaaaagaag aagagataaa
      361 taaacgaaaa cagaaaatat taagtatctt aaatggaact gaagaggatt ggaataacta
      421 caaatggcaa ttatcaaata gaataacaga tgtagatact ttatcaaaaa ttataactct
      481 aactaaaaaa gaaaaagaat atataaaaga ggttggtact caatttagat gggcaatatc
      541 tccatattat ttgagtctta tagacccaga agatatatgt gacccaataa aattactgtc
      601 tataccaaca catatagagt tggaagatga gcaagaagat ttggacccaa tgggagaaga
      661 gtatacaaac ccagcaggat gtataactag aagatacccg gatagattaa taataaatgt
      721 aacaaatgag tgtgctatgt attgtagaca ctgtcagaga agaagaaata ttggacaaca
      781 agattctcat aagtcaaaag ctattatcca agaatctata gactatatca gagaaaatga
      841 agaaataaga gatgtactag taactggtgg agatgctctt acattaaaag atgattattt
      901 agagtggatt cttagccaac ttaaagaaat accacatgtt gattatgtta gattaggtac
      961 taggactctt gttacaatgc cacaaagaat tacagatgaa ttttgcaata tgctaaaaaa
     1021 atatcaccct gtatatataa atactcattt taatcatcca atggaaataa ctaaggaatc
     1081 taaagaagct tgtgaaaagt tagcaaatgc aggagttcca ttaggaaatc aggcagtatt
     1141 attaaatgga ataaataatg ataaatttgt aatgagatgt ttaaatcaag aattactgaa
     1201 aataagagta aaaccttatt atatattcca aagtaaacat gtaaagggaa caaaacattt
     1261 caatacatca gtagatgatg gtcttgaaat catggagtat ttaagaggat atacatcagg
     1321 aatggctata ccaacatata tagtaaatgc tccaaaagga ggaggaaaga ctcctttgct
     1381 tcctcaatac cttgtatcaa aaggaacaga ttacgttatg cttagaacat gggaaggaaa
     1441 agttataaaa atggaagatg aacctgctgt agatataaag aaacttataa aagaacaagc
     1501 acaggattaa aaaaatattt aaagtttata aaaagaaaca actttaaaat aaaaagaaaa
     1561 tggatgtcaa atttatatca atttgcatcc attttttatt agttatttat tattttgttg
     1621 ttgattaact aattcaagat atccacttgg gtggaagtaa tatatctcag aagtattttc
     1681 atttacaaag taaccaatat caccatctgc atctggtaaa aatacataac cattaagatt
     1741 tttagctttt aatgcttcaa aatcactagc atttccttga tatatgtaat caacttcatt
     1801 gttgtatttt actaaaagtt ctttagcttg ttcttgagtc aatttagtat tttggcttac
     1861 aggaacattc ttttgagaat ctgcataaga agattcattt actggtgcag ttaataacaa
     1921 acaagataaa actaataaag atataagtgc ttttttcata ctaaaaccct ctttcatgtt
     1981 taattatata agattcatta cttaataata ttgtatgttt cctataatta aatattacca
     2041 ttaaagagaa taatattaca gttacacagc tgtaactttt gattacaaaa tcgtaattaa
     2101 attgtatttt tattccattt gtattttatc atgtctacga tttacaaact cacagaagta
     2161 tatctttata acgcccatca caggagcaga tagaatcata ccaggtatac caaagaatcc
     2221 tccaccaaca gtaactgcta ggattgtaaa gaatgggcta agacctagtt gtccacctac
     2281 gatttttggc tctataaatg taacttcaac ttgttgaatt attattaagt aaattaaaga
     2341 agttaaagca attgttggat tggagaataa attaattatt acaactggtg ccatacctat
     2401 tactggacca aaatatggaa ccatattcat gacacccata agtgtaccaa ataatagtgc
     2461 atattcagat tttaaaaagt aaagaccaat tccagaaagt actccaacta taaaagaatc
     2521 taaaatctta cctgtaaagt atttaccaat atttaaattt aagctttgac atagttctat
     2581 tataaagtct ccatattttt tacctaagga aatataaact actttttttg taaacagtaa
     2641 gaatttttct ttttctaata atatatagaa acaaattata aaacctaaaa gaaattgtac
     2701 tataaatttt ccaactgaaa atgtagtgct aaatatccca ccaagtgaac caataagtaa
     2761 gttactaaat ttaggcatta cactcataat tttgtcacca tactctttta atgtattagg
     2821 gtcaactgat tgtaaactct tgcctaagtc gaataaaaac tgctctgttt tatctatgta
     2881 tgtaggaatt tgagcaacta aatcagctaa actatttact atggatggaa ctgtaaataa
     2941 tataaaggaa attagtataa ctatgagtac accataagta agaagtaacg aatatattct
     3001 cttaaaattt aatttacttt ctaagaactt tactagtggg ttgaatatat atgctaatac
     3061 aaatgctatt ataaatggtg ttagtagtga cattaataaa cttatcactc caaaaaaata
     3121 tttgtaatta tcaataactt taatcaagat atatgaaaca actacagtga gtacaatatt
     3181 tagataaatt tttttatctt gcaaaataac acaacctttc ttatatttat taaatttaga
     3241 tatacttaaa tctaagttaa agttaatatt tata
//
LOCUS       DAEMHW010000290         3249 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEMHW010000290 DAEMHW010000000
VERSION     DAEMHW010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.292"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            83..1303
                     /locus_tag="KPK67_003632"
     CDS             83..1303
                     /locus_tag="KPK67_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG2460547.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDESYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            1340..2689
                     /locus_tag="KPK67_003633"
     CDS             1340..2689
                     /locus_tag="KPK67_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2460548.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAILLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(2821..3114)
                     /locus_tag="KPK67_003634"
     CDS             complement(2821..3114)
                     /locus_tag="KPK67_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460549.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
BASE COUNT         1244 a          429 c          484 g         1092 t
ORIGIN      
        1 acaaaattct ccatcgaaaa ttatggcata ataattgctt attatattaa taaaacaata
       61 aaaagcaatt ggaggaataa aaatgcaaca ggctttgaaa ttaaaatatc aaaccaaaga
      121 attaggaaaa gattttgtaa tagaaagttg taattctata aaaccatggc ttataaacat
      181 aagaagagaa ttacataaaa ttccagaatt agctcttgaa gaaaatctta ctaaacagaa
      241 ggtaatttct tatttaaaag aaatcggaat tgattatatg gaatttacaa aacacaatgg
      301 aattatggca tatattttaa aagaaagtgc agataaaacg atttgtataa gagcagatat
      361 ggatgctctt cctattgaag aagaaaacaa cataccttat aaatcaatac attctggaaa
      421 aatgcatgct tgtggtcacg atgcacatac aaccatgcta cttggagcat gtaaagtttt
      481 acattcaata aaagataaac ttaatgtaaa tgtaaaattc ttatttcaac cagcagaaga
      541 aggttttgga ggagcaaaat ttttagttga ggatgggtgt ttagaaaacc ccaaggccga
      601 ttatatattc ggacttcatg ttatgccaca tatagaaaca ggccttatcg aaacaaaata
      661 tgatacatta aatgctagtg tagacactat aaaaataagt atcaaaggta aaagagctca
      721 tggtgcatat cctgaaaatg gtattgatgc aatagttaca gcttcacaaa ttgttacatc
      781 attacaaact ataataagta gaaacttaga accaaataat gctgctgttt tgactatagg
      841 taaaatatat ggtggtgatg cacacaatgt aatatgtgaa gatgtaaaac tagaaggcac
      901 tttaagaacc ttaaatagca aaacaagaaa ctttatgatt gataaaatag ctaaaattgt
      961 tggagacact gcatctgcat ttggttgtgt tgggacttta catgtaagtg atgaaagcta
     1021 tcctgctgtt ataaatgaaa aagaattagt agatacagtt atatcaaata ctaaagaatt
     1081 acttggagag gaaaaattta tattgagacc aaacccgtct ttaggtggtg aagacttttc
     1141 tttttacaca gaacattgta aaggagcatt cttccattta ggatgtaaaa atgaggaaaa
     1201 gggcttaatt tcacctcttc atacctctag ctttaatata gatgaagatt gtttaccaat
     1261 aggtgttatg atgcatgtaa tgaatacttt gtattttaat tagtacagta attaataaaa
     1321 gaataggaga taaattaata tggaaaaagc taagaaatta ggtctattta gtatgatatt
     1381 attagggatt aatagcataa taggttcagg tatattctta ctaccaggta aagtttataa
     1441 tctagcaggt caaaacagta tgtttatata catttttgct acactcttag ttctatcaat
     1501 acttttatgt tttgcagaag ttggaagcat gtttgataag aatggaggag catatctata
     1561 ctccaaaaaa gcttttggag attttatagg atttgaagtt ggtacaatgt cttgggttat
     1621 tagaattata tcatggtcta ctctagcagt aggatttgca acagctttag gttcattttg
     1681 gccagaatct gccactgaat acaaaggtta tatagctgca atactcgtaa cactactttc
     1741 aataaatagc ttatttggaa ttaaaagcac aaagataatg aataatgtaa taactatagc
     1801 taaattagta ccattaatag tttttataat agttggaata ttctttataa aatttgtaaa
     1861 tattgttcct tcaggaaatg tagttaactc tagtatgggt ccagccatta tattagtatt
     1921 ttacgcattt accggctttg aatcatttat tgtggcaagt ggagaaatgg aaaatcctaa
     1981 aaaaaatctt ccagtcgcat taataaccac aatatttata tgtgcaataa tttatatatt
     2041 aattcaaatt gtctgtatgg gtatacttgg agatagacta tttgaaaaca gtattccaat
     2101 tgcagatgct tcaagtgtat ttttaggcaa ttatggtaaa gttttcatat ctgtagcaac
     2161 attaatatct atatttggta taaatatagg ttcatcaata gtaactccta aatgtggttc
     2221 ttctctagca gaagaaggtt ctcttcctgc atttatagga aaaacaaata aatatggtgc
     2281 tccatatgta gcaataataa tatctttaat ttgttgtata ccacttgtgc taactggaag
     2341 ttttgaacaa cttgcagtaa tgagtgtaat tgcaagattt gctcagtata taccaacttg
     2401 tttatcagta atagttttaa gaaaaagaac agatgttaag gcctctttta agattccatt
     2461 tggtcctgtt ataccaatgg tagctatttt aggtagctta tggttgttac aacaagcttg
     2521 ggcagaagat atttcaaaac ctataactca aaatagagtt ttaataggat taggggcaat
     2581 attgttaata gctcctctat atatatttat gaaaaaaaat aaggaaatcg agcataaaaa
     2641 taaatcagaa gattctaaat tatatatttc gagagaaaaa attcaataat attttatact
     2701 attacttctc ataaaatata ataaatataa aaaaattgta gataaaacta ttttataaaa
     2761 tttatctaca atttttttat ataaacatat atcaaaatca aaatattatt aatttatact
     2821 ttaaagattc ccatctttat catattttat tcttataaga aaatataatc ctattactac
     2881 taactctatc actaaaaata ctccataggt aagaattgaa tcaatactat ctaacccatt
     2941 tctaaaaaaa attaatgcta tacctgctgc tataatccct atagcatttt ttcttactgc
     3001 attttgccca actccataaa aacctggcat ttttcctttt atttcgacag cagttaaaat
     3061 tatcccaaac actataaata atactccaat aagcatcata atcatactaa tcatctaatt
     3121 acctccttca cttaaggaaa tttcaatttc taaaatctaa aataaataat atttattttt
     3181 tataataata ttgtatatcc aattaactat tttggaaatt agtaccaata aataatgttt
     3241 aaatattga
//
LOCUS       DAEMHW010000291         3249 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEMHW010000291 DAEMHW010000000
VERSION     DAEMHW010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.293"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(46..618)
                     /locus_tag="KPK67_003635"
     CDS             complement(46..618)
                     /locus_tag="KPK67_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG2460550.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(647..913)
                     /locus_tag="KPK67_003636"
     CDS             complement(647..913)
                     /locus_tag="KPK67_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG2460551.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            complement(913..1122)
                     /locus_tag="KPK67_003637"
     CDS             complement(913..1122)
                     /locus_tag="KPK67_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG2460552.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            1391..2251
                     /locus_tag="KPK67_003638"
     CDS             1391..2251
                     /locus_tag="KPK67_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG2460553.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2299..>3249
                     /locus_tag="KPK67_003639"
     CDS             2299..>3249
                     /locus_tag="KPK67_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG2460554.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEYEYKYEIKNIIK"
BASE COUNT         1248 a          376 c          532 g         1093 t
ORIGIN      
        1 taaattataa attatcaaat aataatagtt atacaaataa aaattttaga acattttttg
       61 agattcttct aactcgtaag catccattaa agcttcacca aatctttgat agtgaattat
      121 ttctctttga cccaagaatt ttaatacatc tataacatca acgtcatctg ttaggtttat
      181 aagttgataa taagttgcaa gtgctttttg ttcagctgcc atatcctcat gtaaatcggt
      241 tacgggattt gacataactg ctataggtct tacatcaaat ggaacaccat ttgaatctgt
      301 tggataaatt ccatatccat tttgagcata gtttgaacca agacctgcag cttttaactc
      361 ttctgggctt gcatcagaag ttaactgata aaccatagta catataagct caacatgagc
      421 tagttcttct gtaccaatat ctgttaaaag tgcacgcata ttaccagtag gcattgtata
      481 tctttggctt aaatatctta aagatgcagc aagttctcca tttggcccac caaattgagt
      541 tataagaaat ttagccattc tagggtcaca agtttttata ttaactgggt gttgtatagt
      601 tttctgatat atccacatga attttcaatc ctccaatact tgatgtctaa taattaaatt
      661 ctctgtccca aggccaaggt tggtcagccc attgccaagg gcatctactt ggagcatacc
      721 caaagttagt taaaggaccg tatttatttt cgtaagcata tctagcttgc gcaaattgat
      781 tacataatga attataagtg ttgatagcat ttttatcatc agggtgattg tctaaatata
      841 agttcatatc aacacatgca aaagatgttt cctgaacttt tctcataagt tcagctctag
      901 atgagtcttt cattatcttc tacctcctct atattttttt ggcttagcat ataattcttt
      961 gttgtacatt tcagactcta gtaagtttag ttctggaaat atagttccaa attttaatgc
     1021 atcagaaagg cagtacattt ctgtgaaaat ttgattatta atgtatggtc tagccaaaga
     1081 tggaccacaa ttttttttga tatctctagt cttattttcc aagatgaaaa cctccttgtt
     1141 gtaaatagat aggtaactct atctagtgta taatattcaa tcatttaaaa atcgttacta
     1201 ataataaaat attaaatgag aaaaataaaa aagataataa aagaaagagg ttatttatgt
     1261 tattataagt ttatgtgtta ttgtaaacta taaattattt agtaaaatta aagtaaaaat
     1321 tatagtattt tatggatgaa ggtagacttt tttgtaaaat attattaaca taaatatacg
     1381 aggtgattag atgagtgaac tatcaggaaa aggctgtgaa attatagttc cttttgagga
     1441 aagacttcca gtaagagata tagaaaaaag tataataaaa aaatatagga aaaatttatg
     1501 gtctaagttt atgaaggcaa taagagatta taaattggtt gaagaaggtg ataagatagc
     1561 tgtagcgatt tctggtggaa aagacagtat tcttatggct aaaatgtttc aagagttaaa
     1621 aaaacatgga caagttaatt ttgatgttga atttattgct atggaccctg gatatcatgc
     1681 aaatataaga caattactta tagataattg tgagtatcta aatataccaa tacatttatt
     1741 tgattcaagg atatttgaga tagcagatga aatagcaaaa gattatccat gctatatgtg
     1801 tgcaagaatg aggagaggag cattatattc aaaggcagaa gaattagggt gtaacaaact
     1861 tgcattaggt catcattatg acgatgtaat agagacaaca atgcttaact tattgtgtgc
     1921 tggaaatttt aaaacaatgc ttcctaaact taattctact aattttgaag gtataaaaat
     1981 tataagacca ctttattata taagagaaga acatattata agatttattc aaaatagtgg
     2041 tatatggcca ttaaattgtg catgtatggt tgctgctaaa aagacaggta ataaaagata
     2101 tgagataaaa gacttaataa agagcttaga atcaaatttt aaaaatgtag aaaaatctat
     2161 atttaaggcg gcagaaaatg taaatcttga ttcagtactt ggttggcaaa aagatgggga
     2221 gaaacattct ttcttagaaa attttgaata aaaatagtta aaaagaatca ctataaacaa
     2281 ttggaaaggg tgtttatatt gaaaagagta tatatagatt ttgaaatgaa tatgccaaat
     2341 tctaaatcaa aaagagcagt atttaattct gatataattg caatcggagc agttatgtat
     2401 gatgaaaaaa caaaaaatat tgataaattt aaatctttaa taagaccagt aagtaatgag
     2461 gagttgtatc ctcatataca agaattaaca catatatctt ctgaagaatt gaagagtgca
     2521 ccaagttatg aagaggtcat gagaaagttt aaaaaatggc ttggcatatt ttctgatatt
     2581 aaaggaatat atacttttgg taatttggat ttgacatgtt tcaataacac agatatgaga
     2641 agtgctaaaa aaaataacca tccaaggttt gtaaataaca ttagagattt atttgtagat
     2701 ataaaagaaa aatatataaa ttgtggtatg agatgtatga actatatatc attgaaaaat
     2761 ttacttgaat ttgttaactt agagtttagt ggagatgccc atgacccatt aaatgatgca
     2821 tataatttat ttattttgga tgaggcaatc acaaataatg tagacataca aaatctttta
     2881 ataatacgtg acataataag accaccattt aacgatataa attctaattt agataattgt
     2941 tttaacaaat ttaaggagtc attatataaa aaagaaggaa attataatat tgtagatttt
     3001 tctgttgaaa taataaaaac tgtgagaatg tacttattaa caatcataga tgtaaatata
     3061 caaaatttag aaattatgaa agatataagt aaaaaaatgg atactataga taaattgaaa
     3121 gatatagagg aaggctattt ttatttatta gaagatgtgt acttcgacat gaaagatgta
     3181 ctagaagatt taatgcttta tagaatgaat gaatatgagt acaagtatga gataaaaaat
     3241 ataataaaa
//
LOCUS       DAEMHW010000292         3242 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEMHW010000292 DAEMHW010000000
VERSION     DAEMHW010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.294"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..333)
                     /locus_tag="KPK67_003640"
     CDS             complement(<1..333)
                     /locus_tag="KPK67_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBG2460555.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLY"
     gene            complement(337..1473)
                     /locus_tag="KPK67_003641"
     CDS             complement(337..1473)
                     /locus_tag="KPK67_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBG2460556.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYDTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(1505..2050)
                     /locus_tag="KPK67_003642"
     CDS             complement(1505..2050)
                     /locus_tag="KPK67_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBG2460557.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGIPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            2374..3153
                     /locus_tag="KPK67_003643"
     CDS             2374..3153
                     /locus_tag="KPK67_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460558.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIIEHLLDTSKQNEDKKIYNYL
                     LSLNKGYSLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
BASE COUNT         1170 a          492 c          371 g         1209 t
ORIGIN      
        1 atataaatca ataacgtcca tagattcatc agatataatg attgaaatat tattttttaa
       61 taaaatatca tctaatactt ttttagaaag attataatga caaataatag gaatattttt
      121 tgaataatta atagcaaaaa aactaatcaa ctgaaacatt atatttttag aatatattaa
      181 aattgggata ttttcaccta aagaaatttg ttttcctagt tcctcactat aactcaatat
      241 attttcataa gtatattttt ctccatctat aattaaaaaa tttttatttt tatgcctctc
      301 tttttttatt cttaaaagtt cataatagtt cataatctac accctttcaa gcagtaaagc
      361 tgaacctaga ccaccagctg cagctataga gcaaattcca tatctaccct tagtcttttc
      421 taatgctttt aaaagatgta atgcaataat tgcacctgaa gcaccatatg gatgtccata
      481 cgctaaagct ccaccaaata tattatatct atctattaac tctggatatt ttctttgaaa
      541 caacacatct ataactgcaa atgcttcatt aaattcaaca gcagatacat ctaaataatt
      601 aagactttct atctctaaaa gtttatccat tgcttgtatc gctgatgttg gacttaaggt
      661 tgcatctgtt cctattgtgc atgtattaat aatttttgct ttaggcttct tttttacatg
      721 ttctaaatac ttcttagaac atattataat aaatgaagct ccatcattaa taagacatga
      781 atttgctgca tttgtaatag tttcttttcc taaaattgat ggcatcctat ctaataacct
      841 ttgactcatc ttatcacgta taccttcatc ataggtacta ttatttatag gagatataat
      901 atcctcaagt attttttctt ctcttgcttc ttttgctctt ttatgacttt cctttaccca
      961 aaaatccaag tctgctttct caatttgata tagctctgct actctttccg ccccttcgag
     1021 catactattt tggctattat catcaggaga aaattgagca actgtataat ttggattatt
     1081 tgtatcatat cgcttatcat tcttatgata tgtgcgcatt ggttgtagag aactactttc
     1141 aaaaccacct gcaataatta aatcacactg accagattta acctttgaaa aagctatgtc
     1201 tatactcatc atagcagaag cacactgcat atccacagta aaagcaggta cttcatttga
     1261 cactcctgct gttaaagtca ttaatctagc tatattccct cctgtcccaa ctgcatttcc
     1321 acatataatc tcatcaattt tatcaatttc atacttttta attaaatctt ttaaaacact
     1381 tgctcctagt aattcaggtt gaacaaattg aaaaattcca ttttttagac ctatatgact
     1441 tctcaatcct cctaatataa aaacttcctt catacattac acccttcctt atattataga
     1501 tttatcatag atttcttctg agtacattgt tcagtttagt gcctaaaaaa gatgcaataa
     1561 ccgactttat tgtatctcca attaaaaatg gtactactgc tgcaaaaagt gctgcttcta
     1621 ctgtcatttg attaaaatag cacataaata ctgttcccat aaaatatata ataggtattc
     1681 caacaaaaat tgttattagt atatatctct taatatcatt atttcgtcct tttaataaac
     1741 taataattag tgctgcaaac aaaaacccaa aataaaaacc acctgttggg cttaacaact
     1801 ttccaatacc agatgtacct ccaccaaata ctggaatccc aattaaacct atcattatat
     1861 atacaccaac tgtactaaat gcttgtcttg gtgttaaaat taaagctatt aaatttatca
     1921 ttattgtttg tgctgttact cctgctggag taaatggcaa tggtatatat atgtaagatg
     1981 atatacacaa taatgctaca catattgaca ttttagttaa atcttgaata tttactttag
     2041 tatttttcat ttttataatt cccttcatat gtcttatttc atttagttta tatgtacata
     2101 tacaaagctt ctattaaagc tttgttttag tataaaataa agacgttttt ttgtcaacta
     2161 ttataagtat ttaggtttac atttaattta taagtaaact ttatactgtt actttatact
     2221 ttaatagtca tttataaaac tttgcttaaa aacagctcta tttagactat acttgtaaat
     2281 tataatatga tatattttta tataagattt tattttatat aaaaaatagt aacaaatagt
     2341 ttatacacaa gatatttaaa aggaagtgat gatatgaaag ttaatgaaaa taatataaat
     2401 aatatagatg aagagttaga tgaacttagt aggaaagttt acttagagtc cccagatttt
     2461 tggataagta tttctgacaa aataatagat aaagtatttg atgaaatgtt attatttttt
     2521 gcagttaaat ataactattt caacatagta tgctttgcta ttgaaaacaa ttatctggat
     2581 ttaaacatgc cttcaaaaaa caaaaaatac actaatataa tagagcattt gttagataca
     2641 tcaaaacaga atgaagataa aaaaatttat aattatcttc taagcttaaa taaaggttat
     2701 agcctagatg aaagaccaat tttagatgaa ttaaactgta atattagtag tgatactttt
     2761 gaaaaaaaag atatatatct tccaaagtac ttatgtccaa gttgtaattc taatatattt
     2821 gatacaggtt ataaagtatc aaatgacata acatataaat tttcttctaa agaagctgaa
     2881 cctattgaaa tgtgtagtga agtttcttct atcagatgtt gtaattgtga tgaattatta
     2941 gaagatatta cacttgataa actatataac ttatgcaaaa ttcaaaactg taataattgt
     3001 ggttctaatt taactaaggt aggaattatt gcaaagaaaa aaatgaactt tgatgagaat
     3061 actgaaaaat ttaaagatgt ctcaacatct tactgttgtg gaaattgtga ctctgaaata
     3121 aacttatctc aaagagaaca ttttaagatt taaatattta taggctaaag taataagatt
     3181 acattattag atactgtata agttttttaa atctgtaaaa ataaatacac ttatacgaat
     3241 ta
//
LOCUS       DAEMHW010000293         3241 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEMHW010000293 DAEMHW010000000
VERSION     DAEMHW010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.295"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(179..2833)
                     /gene="adhE"
                     /locus_tag="KPK67_003644"
                     /gene_synonym="adhC"
     CDS             complement(179..2833)
                     /gene="adhE"
                     /locus_tag="KPK67_003644"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG2460559.1"
                     /translation="MAHKEQVIIENKKQNEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYVE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT         1037 a          590 c          382 g         1232 t
ORIGIN      
        1 acacataaaa tcaacataat aaccataaaa acattctttt attattataa taacactatt
       61 taattgaatt ttaaattaaa gaataaaaac tatccatttt ctcaaacaaa aaaactctta
      121 tacaataaaa ctttgtttta tgtataagag tttttatttt atctatttta tagcaaactt
      181 atcttccata atatgaattt aaatacatat cttttatttc attcataagt ggatatcttg
      241 gatttgctcc tgtacattgg tcatcaaatg cttgaattac catttcatct attgaatcca
      301 taaacttaga ttcttcaact ccaaactctt ttatagattt aggaatacca actttaagtt
      361 ttaaatcttc aatagctacc aataaacttt ggaatttctc ttcatcagaa tttcctttta
      421 atcctaaaaa tgaagctatt tcaacatatc tctttaatgc ttctggatat ttatattgag
      481 agaatgctcc cattttcttt ggtgcatctg aagaattaaa tttcataact tctgttatca
      541 aaagagcatt tgcaacacca tgtggtacat ggtggaaagc acctaattta tgagccatag
      601 agtgacaaac acccaaaaat gcatttgcaa aagccatacc tgccattgta gaagcatttg
      661 ccattttttc tctagcttct gggtcttttg caccattatc atatgcacgt ggaagatatt
      721 caaatatact cttaatagct tgtaacgcta atccatttgt ataatcggtt gcaagcatag
      781 atacatatgc ttctaatgca tgagttaaag catctatacc agaagcagat gttaagcttt
      841 ttggcatatt catcatcata tcagaatcaa ctatagccat atttggcatc agttcataat
      901 ctgctaatgg atattttact cctgtatctt ggtcagttat aacagcaaat ggtgttactt
      961 ctgaaccagt acctgctgat gttggtatag ctacaaaatt agctttttca cccattttag
     1021 ggaatgtata tacccttttt cttatatcca taaatctcat agctaggtct tggaaatcta
     1081 catctggatg ctcatacaat gtccacatta tctttcctgc atccattgca cttcctccac
     1141 ctatagatat tattacatct ggattaaatt ctctcatagc tttagcgcct atcttagcac
     1201 attccaaagt tgggtctggt tctacttcaa agaatgtagt gtgcttaata ttcatttcat
     1261 ctaataaatc agtaactgct ttagtatatc cattattgta aaggaatgta tctgttacaa
     1321 taaaagctct cttcttattc attacatctt taagttcttt taatgctact cctaaacaac
     1381 cttttttgaa ataaactttt tctggtgctc taaaccaaag catattttct ctcctctcag
     1441 ctactgtttt aatattaagt aaatgcttaa ctcctacatt ttcagatact gaatttccac
     1501 cccaagaccc acatcctaaa gtaagagaag gagctagttt aaaattatat aagtccccta
     1561 taccaccttg agaagaagga gtattaatta aaattctaca agttttcatg gctgaagtaa
     1621 acttatcaag tttttctctt tgatttacat catcaatata taatgaagat gtatgcccaa
     1681 atccaccatc ttctactaat ttttctgctt ttctaactgc atcatcaaaa tcacttgcct
     1741 tgtacattgc aagtactggt gaaagtttct catgagcaaa agcttcttct atttcaactg
     1801 attctacttc acctattaaa accttagtat ctactgggac ttcaaagcct gcaagttttg
     1861 ctatagtaca agctgtttgc ccaactattt tagaatttaa tcctccattt actaaaatta
     1921 tatttcttat tttttctgtt tcatctttat ctaataaata agcaccacgc tctttaaatt
     1981 cttttttgac ttcattgtat atattttcta atactataac cgattgttca gaagcacata
     2041 tcataccatt gtcaaaagtc ttagatacaa gaattgagtt tacagcagtt tttatatctg
     2101 cactatcatc aattattgct ggagtattac ctgcaccaac tccaatagct ggttttccag
     2161 atgaatatgc tgatttaacc attccaggac ctcctgtagc aagggtcaaa tccacttctt
     2221 tcataacagt tgtagttaat tctaaagatg gctcatctat ccatcctatt ataccttcag
     2281 gagctccagc taaaacagca gcctcaagaa caacttttgc agcctctata gtagaatttt
     2341 ttgctcttgg atgtggagag aaaattattc catttctagt ttttaaagct aaaagtgctt
     2401 taaagatagc agtagaagtt ggattagttg taggtacaac tgctgcaata acacctattg
     2461 gttcagcaat tttagtcata ccaaatgctt cgtctttctc tataacacca caagttttag
     2521 tttctttata tgcattatat atatactctg aggcataatg attctttata accttatctt
     2581 cagcaattcc cataccagtt tctttttgtg ccattatagc tagaggaact ctttgcttat
     2641 tagctgccaa agatgctgct aaaaaaattt tatctacctg ctcttgacta tatgtagcaa
     2701 atattttttg agcttctctg atttttgcta gttttttaac taagctttca acgttattta
     2761 cagtatctaa accaactgac ataacttcat tttgtttttt attttctatt attacttgtt
     2821 ctttatgagc cattttaaca acctcctgag atagtttttt atttattatg ttaatttatt
     2881 aacattatag catttacttt tcctgttttc aagttatttt tttaacattt tattttttat
     2941 ctaatataac atcatttcta ctaataatat ctagctttac aaatacttaa tttaactaga
     3001 tatcacttta ttttttacgc aaatcactaa tgaaatcatc taaaaactta aggataacgt
     3061 tattttttta acgtttatat ttttgtattt tttgcaaata aaactttata aaaagaattt
     3121 ttaatatgat actctatcaa gtttaaaaat aaaaatcctt gaaaatgaaa attaaaactt
     3181 aattaacaag ttaaagtaaa tatagttaaa tgtacaaaca tttacaacat atagtataat
     3241 t
//
LOCUS       DAEMHW010000294         3235 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEMHW010000294 DAEMHW010000000
VERSION     DAEMHW010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.296"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            195..1637
                     /locus_tag="KPK67_003645"
     CDS             195..1637
                     /locus_tag="KPK67_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG2460560.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1679..2032
                     /locus_tag="KPK67_003646"
     CDS             1679..2032
                     /locus_tag="KPK67_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460561.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYCKIKTFKN"
     gene            2223..3044
                     /locus_tag="KPK67_003647"
     CDS             2223..3044
                     /locus_tag="KPK67_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG2460562.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT         1246 a          327 c          564 g         1098 t
ORIGIN      
        1 aacaaaaatc tagtaaattg taactaaaat gtaaattttt cctttaatct atacaatatt
       61 atggaaactt tttgtaattt atgatataat aacaaatata taattattaa attataataa
      121 atgactatag cgtagatgta atactatgtt atagtcattt ttgaattaaa cttgatatat
      181 caagggggaa gaatatgaga acaattagaa gagttttagc attaggttta acattagcta
      241 tatttctaat aaatgtacct aatgtagatg cattaacatc agatacaatt aaaggtaaca
      301 atatatatga gacggctgga ttaatagcag ataaaaagag ttatgataca gcaattatgg
      361 taaatatgga taattctata gcagatggtc tttcagcaag tggtcttgca ggtgctgttg
      421 atgctccaat tttgcttgca caaaaaaata agataccaaa tgaaacaaaa caaagattaa
      481 aaaatgttaa aaagatatac ataataggta aagaattatc aataagtaag tcagtagaaa
      541 ctgaactaaa aaacacaggg gcacaggtaa ctagattggg tggagatgac agaataaaga
      601 ctagttatag tgttgctaaa gaggtaaatg gtatcaaaaa agttgatgaa gtgatattaa
      661 ctaatgcata taaaggagaa gcagatacaa taagtgctgc acccgtatca gtaagagata
      721 tagctcctat agtacttaca gatggaaaga gtgtgccttt ttcaacaagc aatgtaaaaa
      781 catatgcagt aggtgggagt atatcaatga gcactagttt agttaataaa actaatgcta
      841 aaagacttgg tggttcagat agatatgata caaataagaa agttataaaa gagttttatc
      901 cagatgcatc agaattttat ttgagtgatg gatatgattt agtaaatgca cttacaggtt
      961 ctacaattgc taaggaaaat ccaattgtat tggtgtcaga aagtagtgat aagtctatat
     1021 tagcaggagc agataagatt actagattag gttcaataag tgacagtgtg tataataagt
     1081 gtgtttctgc tgcacaaaat aatggcgatt cgtctacaaa aggtgagtca cctatgaaaa
     1141 atgagacaag catattaggt cagccaacag ctagtttaga agcgtgctta aaatgggcaa
     1201 aatctaaaaa agctaatgat ttatttatag agttaatacc aatattatat gatactgctg
     1261 ttcaggaagg tgttaatcct gttttggcag tagctcaatc tgcaaaagaa actggttttt
     1321 gtaattttgg tggagtatta gatgcatcat ttaagaatcc ttgtggactt aaaacttctg
     1381 taggtggttc tgatactgat aagaatgccc attcaagatt tgatacttgg gaagaaggaa
     1441 tattagctca aattcaacat ttatgcttat atgcaggaca agatggttac ccactttcaa
     1501 atccagtaga ccctagacat gaaaaatctt tatttggtaa ggctaaaaca gttgaaagtt
     1561 tatctaataa ttgggctgga ggtcaatatg gacaagattt ggtaagaatg atgggagaaa
     1621 ttgaggcaac aaaataattg aagattatca aatatccaat aaataaggat tgattattat
     1681 gaaaaagagt aatattttta gtatcatatt tcctacagtc ataatggtgc taatgttttt
     1741 aacttttgct acagatatat ttaatattcc agatattcaa tctaaaagta tttttgttgt
     1801 gggaatggtt ttgatttttc caatttcttt tttgatacaa gggataattt gtgtattaaa
     1861 taagactaat tggattttat ctcttattgt gtctttagta acttatatag tattaatgta
     1921 tatattttta aatgattctg catatatata tgttttactt tatacagctt tttatatgat
     1981 tggacatata acagctaaac tctattgcaa aattaagact tttaaaaatt aaattgaagt
     2041 atataaaagt aacattagga gaagtatcct taatgatttt ctaaaatcag aaataggtgc
     2101 ttctcttttt tgtaaaaata aaagtatgta aataaaattt aaccattata attttctttt
     2161 aacaaatgta tacaatgaac ttgttgtata attaagatag ataaaataaa gggagggata
     2221 atatgaattt aatagataat catattcata ctaatttttc aagtgatgga aaggattcca
     2281 tggaagatac aataaaaaaa gctataagta ttggtgttag atatttgact tttacagacc
     2341 atttagaaca tgatgaagat agaggatttt gcataaatta taatgactat gttccagtat
     2401 ttaataagtt taaagaaaaa tataaaaagg acatagagtt attactaggt gttgaagtgg
     2461 gttacagaaa acatctaaaa aatgaaatag aagaaataat aaattctaat ccttttgatt
     2521 ttgtattgtg ctcaactcat acaattgaca atgtacctgt tccatcaaag gcgtatttta
     2581 aaggtcttag caaagaagat gcgtattata aatattttaa tagcatactt gaaactaatc
     2641 gtgaatttgg agattacaat atatatggtc atttggatta tatatctagg tatggaatat
     2701 attcagataa tagagttata tacaatgact ttaaagatgt tattgatgag gtcttaaagt
     2761 caataatcaa taatgggagt ggtatagagc taaatacttc tggttataga tatggactaa
     2821 atgctataca tccaaatgaa gatattctaa aaagatataa agaacttgga ggatttatag
     2881 ttactgtagg ttctgattct catagggtag aagatatatg caaagatttt gatgtggcct
     2941 atgatatgct taagtatctt gactttaaat atgtatcttt atttaaagaa agagaaactt
     3001 attttgtaaa tatagaaaaa gttaaatcta acaatatagc ttagagtgtg ttttgaagta
     3061 aattaatgga tagttaaaaa gttaatagtt ataatattgt atcctctaga aaaatactag
     3121 aggtttttat tttgctaaaa taactaaata gaattaaatt taaatagtat agcttatttt
     3181 aaatttaata gtttattgtt gatatatttt actattatat atttttattg tgata
//
LOCUS       DAEMHW010000295         3230 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEMHW010000295 DAEMHW010000000
VERSION     DAEMHW010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.297"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            29..727
                     /locus_tag="KPK67_003648"
     CDS             29..727
                     /locus_tag="KPK67_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460563.1"
                     /translation="MKISSQYRSQYSFRHESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            905..1795
                     /locus_tag="KPK67_003649"
     CDS             905..1795
                     /locus_tag="KPK67_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460564.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQVDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(2196..3152)
                     /locus_tag="KPK67_003650"
     CDS             complement(2196..3152)
                     /locus_tag="KPK67_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG2460565.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
BASE COUNT         1259 a          370 c          485 g         1116 t
ORIGIN      
        1 ataaatttat tttagggagg tgaaataaat gaaaatatct agtcaatata gaagtcaata
       61 ttcatttaga catgaaagta atataaataa tacaagaata aatgaaagta tggttaagaa
      121 aaatgaaact gtaggaaaag acacttattt atctaatatt atgaaacaaa agcaagaact
      181 taatgataga attagagatt taaaatatag acaagaggtt tatactaaaa aaataaatga
      241 cgcaattaag aacttatgta aatcagaaat aagagaaaca actaataact tttctaatat
      301 agaaataggt attaaaaata gcattataga agataaaaat aaaagtacaa tgttagatga
      361 aaattcaact tatctaaata caaatgatga aaaagaatct ttaatcacta aagaatctaa
      421 tgaaaaaatt gaagaagaaa tattaaatga tgaaaaatta gaagagttag aacaaaaaaa
      481 ggattataaa gaggattcta ataaaaaaga gaaagtatct gaggacttat ctttagtagg
      541 taaaactcgt gaagagcttg aaaatatgct taaaaatttt ataaatttaa cacaagaaga
      601 aataatgaaa cttgagtcga gaatagaaaa gttagataaa aatgctgaag aatacaaaca
      661 aaattcaaag actaatatat ttgataaaac agatgaacaa aaaaaacata taaatgtact
      721 gatttaaaca atatgtttta aaaaaataag tttattacat aatgaaaata taaaggtata
      781 atttcaatac acttctatta aaccaataat ttatgaatca gatttaattt caatttacta
      841 ataaccatat atttatgagt taataatttt atgttttaaa aagaattaaa taagggggag
      901 tagtatgaag aagaatatat taactactat gattaccatg attattatat tgatgatgag
      961 tatgacagta atggcagagc catatcaaga agatgaaaca tctagcttga aaaaacagag
     1021 tgcaaatgaa cgtaatcaga ctaatatagg cacaatggat ggcagagtgc aaacaaatgc
     1081 aataagtaaa ggtgctaatt atgaccctaa atatcctctt aaaggaatgt taacacaact
     1141 tggtcttaat acaacaggta aagagggatg ggatttactc tatggaggaa atcccaatgg
     1201 agttgagctt cgagcagatg gtaaatatta ttatataaat aagggtatgg aagggctagg
     1261 attttatgcg gcagcattgt caggtggctc atatcagaga ataggctttg gaaaacttga
     1321 ccctatagaa gtaatagctg atggaaattg gatttcttac aaacaagctc aagatacact
     1381 tacagtaatc aggaattttt taaatagttt tgactggaga aatgcatctg agatggaaag
     1441 agctaataga gctgcaaaat tggtaaccga agcaaaatat gcagactcta aatattgtaa
     1501 catagtctat ggtaatctgg ttgataaacg tggtgtatgt ggaagttttg caagtagctt
     1561 tcatttatta acaaggttaa tgggaatgga ttcactatct atattaaatc catcattaaa
     1621 tcatgcttgg aactatatac aagttgatgg gaaatggtat aggtttgatg ggtctgaaat
     1681 atcagctttt ggtggatcat tagactttga ttaccgtaaa ttaaaggatg caacaagaga
     1741 gatgactact tactatgatg ctaaggcatt gtctatatta ggatttaatc aataaaaata
     1801 ggggaggctt cttatggagc ctttttttat tgatttgaaa tttaataagg aattttatat
     1861 gaaaatttaa tcaataaaag caaaaaaggc tctttataaa gcctttctaa gtgttttttt
     1921 aagtaaaatt ttttatatca acatcagttt taatcaaaaa atataacatt ttggatttac
     1981 ttttaaaaat aaaattatta aaatataata ttaatcatgt taataatatt tttagtttat
     2041 tatattctat aatagtggtc atgttgacta ttgtagtttt attaagaata gttggtttat
     2101 ataaatatat gcaaagatag cttgttgttt actttactta ttagtatata ggacaagata
     2161 ctctaataaa tttgcctatt ttaatatctt ttgtactaaa accaactaaa gtttccttta
     2221 ttatttcgtt ttgaataata caaagcataa tccgattgtt tgtataaaga ctggaaagta
     2281 tcagagttat tttctaattt agatatacct atagaacaag aattactctc taggcataat
     2341 tctaaaactt tctttgcttt attttcaaca ttctcttttg attgattact atattcaaaa
     2401 acagcaaatt catctccacc aagtcttcca ataatagcat catttacgaa tactttagtc
     2461 aaaatatttg aaaaatgtat tagtatttca tctcctcgaa catgaccaaa gatatcattt
     2521 acctgtttaa aattatctaa atcaaagatg taaaagtatc catgtgaaac agttttaagc
     2581 atttctttta tgtgtacttc aaaaaaatgt ctattgtata gtccagtcat aaggtctttt
     2641 tctgatttta gtttaaattc atttgccatt gctagtgctt cttgtgcttt ttcagttatt
     2701 gttttagggt aatactcatc tttcatttgg tcaaagtttg gaatagaatg gtgaacatgg
     2761 ataagtttaa tcatatcatc tgtaactctg tataccatag taaatcgagt atccatttct
     2821 acatagatat ctttttctag gtaatctttt tctattaccc aaagagtacc atatactata
     2881 aaagcttcgt ctgtaacttt ggtacatgaa taccactcat ctataatctc aaagtctatt
     2941 tgctctgatt ctgtttggtc tttttcaaga cctataataa attcttctaa tgtttcgtaa
     3001 aattcatgtt ttcctgttcc aattaatgca aggttgtcat ctaataagga aataataaat
     3061 ttaaggtttt cttcttctgg atttaaaata taaatatgcc aaattttttt tgataaatct
     3121 cttaattgtt tttgtattgt attattttcc atagattacc cccttaagat ttacagttta
     3181 tattttaatg tattataagt tgaactctta ttacaatatt attataatat
//
LOCUS       DAEMHW010000296         3144 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEMHW010000296 DAEMHW010000000
VERSION     DAEMHW010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.298"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            221..934
                     /locus_tag="KPK67_003651"
     CDS             221..934
                     /locus_tag="KPK67_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2460566.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(1206..2744)
                     /locus_tag="KPK67_003652"
     CDS             complement(1206..2744)
                     /locus_tag="KPK67_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG2460567.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         1087 a          435 c          449 g         1173 t
ORIGIN      
        1 agtctaatat tataaacttt ggatgaaatt aattttctat aggttttaat gtatcataca
       61 ctttatttta aagaaactta tcttaatttt catagtgatt gaatttgtct attagagatg
      121 ttactattag taatgtaaaa gcaagagtag tgaatataaa aaacaagata gtgatactgc
      181 aagattagaa tataataaat ttttatggag agatttcaat atggatacat gttttaataa
      241 aaaaatatta cttgttgatg atgaaaaaga tatagtagat ttaattgaag aagtactcat
      301 aaatgatggt tttaaaaaca ttattaaagc ttataatggt ttagatgcta tttcgctgtg
      361 taagatagca tgtccagatg ttgtcattct tgatataatg ttaccagata tagatggtat
      421 agaagtgtgt aaaaaaattc gagagttttc atactgttct attctttttt tatcatcaaa
      481 aaatgatgat attgacaaaa tactaggtct tagtagtggg ggagatgact atataacaaa
      541 gccattcagt ccaagagaaa ttgcttttcg agttaaagcg caattacgta gacaacagta
      601 tcaaagcatt gtaccatcag atagtgaagt tattaaaata ggagatatta ctattgatat
      661 agaaggaaac cgtgtatata aagatagaaa tgagattgag ttaactggaa gggagtatca
      721 tcttttaagc tacatggctc aaaatgtaaa taaaattata ggaaaagaaa gactctatga
      781 acaagtatgg ggagtatata gtagtatttg tgacaataca attatggtgc atatacgaca
      841 tataagagaa aaaattgaag ataacccctc aaatcctaaa atacttataa cagttaaagg
      901 actaggatat aaacttgtga atagaatcga ttagaataga ttttaatatt aatgaagata
      961 tctcaagttg aatttttagt taatgaggca ttcttttatg ggtgaaatca aatgtatttt
     1021 cgttatctca acaatgaaga agaggtctat ctctattttg agatagcctc attaagtttg
     1081 tatcctggta atgtacttta tgttctaata aaattttttt agtttatttg cagtgaaaca
     1141 ttaactagaa tattaaaatt tgcattgaaa taccaattaa ataatattgt atattgtgta
     1201 taaacttata ctaattgttt ttctatatta aatactttat caatccaacc tttataaaat
     1261 ttttcttcat gggaaactag gattatactt cctctaaact gtttcaaagc attttgtaaa
     1321 gcatcctttg tctctgcatc aaagtgattg gttggttcat ccagaataag aaaattacaa
     1381 ggagataaca tcatacaaca taatttaact ttagattgtt caccaccact taaagtagat
     1441 acactccttg aaacatgctc ttccttcaca ccacatctgg ctagatgacg tctaatctct
     1501 tttgtattta attttggata tttatctgct actatttgaa gtggggtttt atcaggattt
     1561 tcccatttta aatcttgctc ataataacca attttaactt gttctgaaaa ctggaaatct
     1621 ccagatatac gaggaatatc tttgactaat gtctttaaca aggtagattt accgattcca
     1681 ttaaaaccag ttataactat tttttgacct ccatcaacag agaaatttaa ttttggaagt
     1741 aaagaatagt agtatccaac ttcaagattt gttatagtaa gagcattttg agccgatatt
     1801 tcaatttctg aaaattgaat atttggtttt cctgtaaaac taggaggagc tattctttct
     1861 attttatcca attgtttttg tcttcctctt gcaatttttg aatttactcc agctttattt
     1921 ttacgtataa atgtttcttc tttttctatt ttcttttgtt gtgcttgata ccttctaata
     1981 taatcttctc ttaaatgtga tttttgttta aggaactctg aatattttcc atgatatttc
     2041 ttaatcatgc caaattctat atcaagaata cctgtagaaa ttttttctaa aaagtcaaaa
     2101 tcgtgggaaa ctacaataaa tgccccatta aatgtattta aataatttga gagccattca
     2161 acatgttctt tatctaaaaa gtttgttggt tcatctaata atagaacatc atggttagaa
     2221 agtagtaatt ttgctaaaat tacctttgcc ctttgaccac cactaagctc tccaacaaca
     2281 cgattcattc ctattgaatc taatccaaga ccatttgcta ttttatttat ttcattatct
     2341 atagaataga aattacttgc tattagttgt tcctgataat ctgatgcttt gattagatat
     2401 tgctcatttt cagatatagc acttttttga tagagtaaat tcatttcctt ttctatttta
     2461 taaagttctt caaatgcagt gtgtaaatag agtgatattg ttgtatctct attgatttct
     2521 gcatattggt ctaaatggcc tatattgata ttaggttgcc acttgatttc tcctgaatct
     2581 ggaacaactt ctcccaataa aattttaatt aatgtacttt tacctgtacc attttggcca
     2641 actacaccta tatgttcacc tttgaataaa tcaaagttag ctttctcata cagtgattta
     2701 tccataaaac attgtgataa attagttact tttaataaac tcataattca aactcctttt
     2761 cttttattaa aaaatttatg gaaataaaaa aagcagagga atggcattgt aaaatgccga
     2821 cctctgcttt tggaaaaaca aaaggtcata gacaagcatt gttgtctatg accagaaaat
     2881 ctatattatc tatcggcaaa taggtaattt taaataagca tagccaaaaa actatactaa
     2941 acctatattt gccgatgaag atagaaagct ttaagctttc agattaacct ctacacaacg
     3001 cttcatcgta tacatttgtg tagagttaag ttttctaaca agtttaacta attctatata
     3061 aatcataatt attcacatcc taatcttagt attaattcta gtttatctat atttaaattg
     3121 tacttttgta ctttgtggat tatc
//
LOCUS       DAEMHW010000297         3133 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEMHW010000297 DAEMHW010000000
VERSION     DAEMHW010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.299"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            140..2152
                     /locus_tag="KPK67_003653"
     CDS             140..2152
                     /locus_tag="KPK67_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2460568.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINIKNNPKTLNRQDLEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRALPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            2394..>3133
                     /locus_tag="KPK67_003654"
     CDS             2394..>3133
                     /locus_tag="KPK67_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460569.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQS"
BASE COUNT         1331 a          359 c          506 g          937 t
ORIGIN      
        1 attttttgag ttttataagt ttatttttat atattattta tatattattt atattttggg
       61 ttagatacac tttgacatat tttcaaaagt taaaagatta aaaataaagt agaaatttat
      121 gttaagtaag gagattaata tgagaagaaa tacaaaatta ttaacaacag ggattctttc
      181 aatggcaatt gttgcaccta caatggcatt tgctactgaa tctaatgcta tggaaaataa
      241 cgctgattta aatataaact tagagaaaaa aagtatcgtt ttaggtagca aatcaaaagt
      301 tagtgtcaaa tttaaagaaa aaccagatgc agatagcatt acattaaagt ataaatgcta
      361 tgacatgcca ttggatacaa ctctaaatta caatcaatca actggggcat atgaaggaac
      421 tatcaattat aaccaagacc cagaatatct aaatgtttgg gaactacaag ggataacaat
      481 aaacatcaaa aataatccta aaactttaaa cagacaagac ctagaaaaga tgggattaaa
      541 tttaaaagac tataatgtaa cacaggaatg tataattgaa gacataactt ctagaaaaga
      601 tgtaaataaa tatttgagaa aaacttcttc acctattaca gaacttacag gaagtgatag
      661 atatgaaaca gcagttaaaa taagtaaaga aggctggaaa aatggttcag ataaagtagt
      721 tataataaat ggagatgtaa gtatagatgg cattatatca actccactgg caaccacata
      781 taatgcacca atacttttgg ttgaaaaaaa caatgtgcct aatagtgtaa aatcagaatt
      841 aaagcgccta aaccctaaag atataattat aattggagat gagaatgcta tttctaaaac
      901 tactgctaat caaattaaat caactgtaaa tgctagtcaa acacgtttaa atggttctaa
      961 tagatatgag acatctttat tgatagcaaa ggaaatagat aaaaatcatg atgtggaaaa
     1021 agtatacata acaaatgcta atggcggaga agtggatgca cttactatag cagcaaaagc
     1081 aggtcaagac aagcaaccaa ttatattaac tgataaagat agtattacag acaatacata
     1141 taaatggtta aagagtgagg atttacaaaa tgcttatttt ataggtggtc ctcaaatgat
     1201 atcaacaaat gttataaata aggtaaatgg aataactaaa gatagtgtta ctaataatag
     1261 agtatacgga gcagatagac acgaaacaaa tgcaaacgta ataaaaaaat tctatacaga
     1321 tgatgagtta gaggctgttt tagtagctaa atcagatgta cttgttgatg ctttagcagc
     1381 aggtccattg gctgcgaact taaaatctcc aatacttata acaccaaaga cgtatgtatc
     1441 tgcataccat aaagataatt tagaagctaa atcagctaat aaggtataca aaataggagg
     1501 aggattgact tctaaggtaa tgagctctat agcatcatca ttatctaaac acaatacgac
     1561 tccaacagaa ccaggaaata gtgggggcaa gacagttatg attgacccag ggcatggtgg
     1621 ttcagcacct ggaaattcat ctggaggaat gattgaaaaa gattacaatt taaatacttc
     1681 acttgcaaca actgaatatt tacgttcaaa gggattcaat gtaataatga caagagacac
     1741 agataagact ttatctcttg gaaatagaac tgctctatct aattcattga aaccagattt
     1801 atttacaagt atacattata atggctcaac taataaacaa ggtcatggtg tagaagtatt
     1861 ttataagctt aaagataaaa atggagggac tactaaaact gtagctacca atatattaaa
     1921 tagaatttta gagaaattta aacttacaaa tagaggtata aaaacaagag cacttcctag
     1981 tgattctaca aaagattatt tatacgtttt aagaagtaat gatatgccag ctgtacttgt
     2041 agaatgtgca tttttggata atgaaaatga tatgagttta ataaactcat ctgcaaaagt
     2101 aaaagaaatg ggtacacaaa taggtaaagg aatagaagat tcattaaaat aaatggaaat
     2161 aaaaatattt aaaaacttaa tgtaaattta tagttgtaaa tttatactaa agtcaaaaaa
     2221 tgaaatataa gtgtatcaat agtaagactg tatgtacatt tgcacatgtg aatgcaagta
     2281 tatatacagt ctttttttgt agttaaatgc aataatttga ttgtgttttg taaaatacac
     2341 aatatgatat aattagataa tatacactta atttaattga ggaaggtgat tatatggaat
     2401 atttatttga aaaaagtagt ttaaaagaat atggaaatga agctttagat aaatatagag
     2461 aattattaga taaaactggt aataattcaa ggtgtattct tttgttagta ccaaacaata
     2521 acacaaagat acgatatgaa agggctctaa gattaaatta tagtgaagaa ctgaaaataa
     2581 caacatatat aagttttgtg aagaaagaat tagttaaata ctggccacta ataatagaaa
     2641 aatgtgatga aataagtaca aaggttgtat ctccaacatt tatatcaaat agtttaagtg
     2701 aatatttaat agtgcaaaaa gttaaggaaa aaaggataca agaaggatat tttgaagata
     2761 ttacaggaac caataaatcc atagcaaata gtataatgac taacataaat aagtctgcat
     2821 ttaatttaat tgattttgat tttattggag aaaaaatata ttcatcaaag aaaaataaag
     2881 atagtatata tagattttca tacactcaaa tggatgaaat tatatcatat tatataaatt
     2941 ccttactatc aaattcaatt ttagataatg cactaagcat atatttatac aatcaatacc
     3001 tacttaatga tgatttttat ttaaaaaact tatttacaga agtgagatat ataattgtgg
     3061 atagccttga aagctcaagt aatgcagaag ttgattttat aacagaagca ttggataata
     3121 cattacaaag tta
//
LOCUS       DAEMHW010000298         3086 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEMHW010000298 DAEMHW010000000
VERSION     DAEMHW010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.300"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(118..942)
                     /locus_tag="KPK67_003655"
                     /pseudo
     CDS             complement(118..942)
                     /locus_tag="KPK67_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
     gene            complement(953..2356)
                     /locus_tag="KPK67_003656"
     CDS             complement(953..2356)
                     /locus_tag="KPK67_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG2460570.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(2360..3085)
                     /locus_tag="KPK67_003657"
     CDS             complement(2360..3085)
                     /locus_tag="KPK67_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2460571.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRRTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDVQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
BASE COUNT         1037 a          522 c          271 g         1256 t
ORIGIN      
        1 taactgaatt tctgctcata aatttaaata ttctacaaat ctcatctaaa taacaatttt
       61 aatagaccta aaacaatcct catattgttc attttgtatt aaaaatatca cttacaatca
      121 aaaaccattt attaagtata ttcctaagat atacaatata tatacatgga ttggatagta
      181 taaataaaat aaatacttca tactcttccc tttttctcca ttgtaaagta acattaaagg
      241 tagagaagca atcatcatcc attggtaatt ttgtaaaata gaatttttta aactataatc
      301 tccatttgac attgttgtaa atataaatat acacattaaa acataacaaa tactcaaact
      361 gaactatttc cctctacaca aataaaaaat aatcccaagt ataatccaaa ttggtccacc
      421 ttctactaaa aatggtactg gtaccaaaat cataaataaa gcaataactc tgttagtgac
      481 tttaaaaagt aaagctactg taaatattcc taaaattata ggaatgatta tcatcaataa
      541 tcctataata aaatatttaa tttctttctc ttttctaaat ttctttatga actctattgc
      601 ttgtaaataa atcgctatta aaaataatgt tgaaaaaatg ttatttataa ttatagctcc
      661 ttctggatgt gaaaatacat tattaattat ttgatttcct atagccataa taacagacca
      721 aatatacaat ctacttatat atttttttct gttactagtg tgataaaatc cttcaacagc
      781 catgaatata aacagtggtg ctgatattct gcctacccaa tgaaaccaaa ttggtatttg
      841 cattgtttgt ggcataaatg ctgctatatg gtcaaatgtc ataaatatta atgctaaaat
      901 cttaatagta aacccactta tacctttttt aaaaaaatcc atatcatatc tcctaatttt
      961 gttttatctc tataataatt tttgttccta ttccaatttc actacttaca taaattttac
     1021 ctttatgaat ctctattatt tctttagaaa tagccatacc aagacctgaa cctttatgca
     1081 tctcacctgt attagtacct ctataatatt ttttaaaaat atgtttcaaa tcacttttgc
     1141 ttattccaat tccattatct tttattataa tttctatatt atcttttttt acaatttcta
     1201 cacttatcaa agttccttcg ctattatgaa ctattgaatt aaatataaga ttggttatag
     1261 cccttctgaa taagattgaa tctacatata cttcaattaa atcttcatta ctttcaaatt
     1321 caatattcct atttttatat ctattatcat ttaaaatatc aattattata tctctgacta
     1381 aactgactaa atttattttt ttcttgtcca gaactatagt attatttttt aatcgagtag
     1441 ataaatttaa atcatcaact aaatctttta tatacttaga cttattatat ataatttctg
     1501 tatactcttg tatttcatct tcttcaaact cataatcttt gtcattaatt atctctgcat
     1561 acccttgtat agatgctaga ggtgtcttta tatcatgtgt tatatttgcc agccattcct
     1621 ctctcaactc ttcattttct tttctctcaa cttcatttac tcttaaagta tcagctaaca
     1681 tatttatatt tttaaaaact tcttcatata tacctctgtc cttaagatat aaactataat
     1741 ttccattagc catagtctca atactccata gtacgcttga aattggtttt gtaagttttc
     1801 tactaaataa atatcctaca cctaaagcta ccacgctatc aattactagt gttataatga
     1861 taactttatt taaagttttt ttaatgttat tttgagaata ggttaaaatt attcttgata
     1921 tatctctact aggtatacct actattatgt ttagatgttt tttgtttaaa tatttttcta
     1981 gtacaaaatt aacatacttt gtttctatat acttgtaatt attcaccatt tgaaagggtg
     2041 tatatttttt tggagtatcc tttggcacat ttactccata aacttcctct cctaaatcat
     2101 ttaaaacctg tatccaggaa tttgattttt ctaatttttc agctccaatc ttagatagtt
     2161 taaattctcc atctttttca tataaatctt tttcaaaaga acgtgcaaat tcttcaggat
     2221 tatttccaga atctacaact ttaaaaaaac tattagttga aatcacatac aatatagata
     2281 tgatatttat aattactact gatatagcta caaacacaat tgtaaatata aaatttctag
     2341 taatcttcca cttcatattc taatcttcct ttactgctaa tttatatcca agacctttta
     2401 ttgtcagaat atattcaggc tttgaaggat tatcctctaa tttttcccta atttttctta
     2461 tatgaaccat tatagtattg tcaaatccaa cataatcttc tccccacact ttatcccata
     2521 gtttttcttt acttataatt tgattttgat tatgtgcaaa taaagatagg atttttagtt
     2581 cctttgcaga cagtcctata gattttccat cttttataag ttctgcccta ctttcattta
     2641 atatatatgg gccaaatgat ataatttttt cttcattatt atttagattt ttactttcgt
     2701 tttgaacgtc actaaaataa cttaatcttc tcaaatgcgc tttaactcta aaagccactt
     2761 ccttagggct aaaaggtttt gtaacataat catctcctcc aatcgctagt ccaagtaact
     2821 tatctaattc ttcggtttta gctgataaaa atattattgg agaattggag gttcttctta
     2881 tttctttaca aatatcataa ccttcgccat ctggtaacat aatatccaat acaatcaaat
     2941 caggattaat acttttaaat aattctaatc cttctttttt agtttttgca gtatatatat
     3001 tattaaggcc ctcttttttt aggacagttt ctaaaagctt caatatttct acttcatcat
     3061 ctattattaa aacacgattt tccata
//
LOCUS       DAEMHW010000299         3080 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEMHW010000299 DAEMHW010000000
VERSION     DAEMHW010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.301"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            239..505
                     /gene="rpsT"
                     /locus_tag="KPK67_003658"
     CDS             239..505
                     /gene="rpsT"
                     /locus_tag="KPK67_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBG2460572.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            complement(692..1735)
                     /gene="holA"
                     /locus_tag="KPK67_003659"
     CDS             complement(692..1735)
                     /gene="holA"
                     /locus_tag="KPK67_003659"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBG2460573.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSVVKKIGNNGIVFDCDKLSDMDLFKWIKKSFDLN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(1689..2990)
                     /locus_tag="KPK67_003660"
     CDS             complement(1689..2990)
                     /locus_tag="KPK67_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG2460574.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
BASE COUNT         1137 a          445 c          283 g         1215 t
ORIGIN      
        1 tatttgttac aaaaataaaa tattaattta tatatgtcaa aatatttaaa acacaaaata
       61 taccaattta aaaacaagat attgcaaatt atttatacac aatttacagt ctaaaaaatc
      121 aaaataaaaa acattattaa aatatataaa taataatcct tgatttttag atttatttct
      181 gttaaaatct aatatgttga cgatgaatat gataaaaaaa tacagggagg tgaacacggt
      241 ggcaaatata aaatcagcta agaaaagaat aagtgttatc gaaaagaaaa ctgctttaaa
      301 tagagttaga aaatctcaaa taaaaactgc aataagaaga ttcgaagatg ctgttgcagc
      361 tggaaacaga gaggatgctg ttgctaagtt ccaatacgct caaaaaagaa tataccaagt
      421 agcttctaaa ggaactatac ataagaatgc tgcagctaga aaagtagcta aattagctca
      481 aaaattaaac ggtatgaacg cttaattacc agtttatatt tcttatatat aataacccct
      541 atgtaggggt tttttatttt gtaaatttat acaataaaaa ataaatagtt agacatatta
      601 tagttgattg tatatcaaac ttaatttagc ttacatctac tctgaactat aacatatctt
      661 gctatttaat ttttattaat atatagattt tttaaattgc ttctctttta cagtatctac
      721 taaccaacat ttcaactgct aaagtatctc ttataagacc tgtctttatt ttataatcac
      781 tttctaatat atagtttaat atttccacaa ttatatcatc agaaaaattt ttagtttgct
      841 ttaatgcttt tcctacaaca aattgatgca ttttcaattt atcagctata agttttgttg
      901 aatatccatc aagttgtaac tgtcttactt gcataattat tttaaattgt cttgctatca
      961 tagaaaatat acctaataca gattcgcctt cttgtatcat atcatttaat attttcattg
     1021 cattagaagc attttgttct ccaatataat cgattaattt gaatatatca ttctcaactt
     1081 ttttttgaga taatttatca attacatcat tggttacatt attcccttta ccaacaaatg
     1141 aacttatttt atttatttca ttttctaaat cagataatgt cttttcacta ctcttatctc
     1201 tgtatccttc ttgttcaata aaatacatga tttgagaatt atctattata acatcattta
     1261 aatcaaaaga tttttttatc catttaaaca aatccatatc agaaagctta tcacaatcaa
     1321 aaacaattcc attgtttcct attttcttaa ctactgattt tcttttgtct acatctccat
     1381 atacaacaaa aactatagtt gtggtgtctg gaatattgtc taaatgctct attaagtatt
     1441 tctcatcact atctgtaaaa ttctttttct ttccttttaa tagttcaaag tcttttatta
     1501 ttactatctt cctatcatcc ataaatggaa gtgtttctat agagcttata agctgattta
     1561 agactatttc cttcccatct attatatcta aattaaaatc aagcattccc tcgtttagac
     1621 tatctttaaa tgcttttatt gcattctcaa ttaaataata ctctcttcca taaaaaagat
     1681 acattttttc aaagtttttc tcttttacat tccttataat atccttgtaa ttcattctct
     1741 tgctccttta tctcttttcc ttctatgtaa aatatgtaaa aaaacactcc tatgtaataa
     1801 ataacaacaa tataaaattt aggattttct atattaatgt atgcaaaact taaattgctg
     1861 attttttcta gtaaaaaaaa tatgctttct atcaatgtac tattaaaaaa agatataatt
     1921 ttagctatta ctacacttac tttaaatacg attaaacttg caatacttaa atacattatt
     1981 actcccacaa atggtactat aattaaattt cctataattg aaagcaatgg tataccttta
     2041 aatgtataat aaattatagg taaagttaat atatttgatg atattgtcaa tgataccata
     2101 cttaatttta taatactatt tattttatta tagaatatta atatagaaag tgtagctaaa
     2161 aaagacaatt gaaaacttac attatatata acataggggt tattcataat taaaattatc
     2221 ccaattagag acaatgccga tattccatct ttctttctat caataaatat tgataaataa
     2281 aaaatcataa caaatgctat agacctaatt atggatggag acgcacctac cattatacaa
     2341 tataaagtta taaaaatcga cagtattaat aatttataca atttatttat acctcttaat
     2401 agaaaggata taattacaca taatattcct gtatgaagac ctgatataga tattacatga
     2461 ctagttccag ttctggtaaa aatttctttt tgttcctcag tcaaattttc aacttctgct
     2521 aatagtatag aatttataaa atctgagttt ttcttatata agtacctata agttttacta
     2581 atataaaact ttatttcccc tattaaagag taaagtttat ttttatcaat aattttatag
     2641 ttatttatat acactatacc tttatatcca gtacttttta tataccttcc ataatcaaaa
     2701 tcatcatact tcattttatc taaacttttg aatttacctg taatattaac tttctgacca
     2761 atcttcaaat ttttattttt tgtataatca tttattagaa aaacccctac cttgtactga
     2821 ttgtatcttt tcttttcaat ttttcctttg acaactcctt gtatagttat tatcttatca
     2881 ttaagttctt cgtcgttttt tgtgttattt gtaatgataa atgatatagc taccacaaac
     2941 attaatataa ttaataatgg tcttcttatt tgaattataa tcactcccat ataaaagtat
     3001 attatttaga ttattttcaa atagaaaatt tttaaacaag ataaattgtt atctaaacaa
     3061 aaaaatacgt caaaagattt
//
LOCUS       DAEMHW010000300         3062 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEMHW010000300 DAEMHW010000000
VERSION     DAEMHW010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.302"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            30..2387
                     /locus_tag="KPK67_003661"
     CDS             30..2387
                     /locus_tag="KPK67_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBG2460575.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYEAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2473..2790)
                     /locus_tag="KPK67_003662"
     CDS             complement(2473..2790)
                     /locus_tag="KPK67_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBG2460576.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1181 a          333 c          496 g         1052 t
ORIGIN      
        1 aaaaagtgct aagttgaaag gagtaaaata tggataaggt taatttagaa aggtatagtt
       61 gtgatataaa tgttggttta aataaaaatc aacttcaaag tagaatagat gataatttaa
      121 ttaatgcatt tgataatgga aaaaccaaaa catataaaca aatttttaaa gacaatatat
      181 ttacactttt taatctcata aatattgttt tagtctgttt attagtttca gttggttctt
      241 ttaaaaatac cttatttata tttattgctg taattaatac tattatagga gtaatacaag
      301 agataagagc taaaagatta ttggacaatc tatctgttat agtttcaaat aaggtatctg
      361 tcataagaga aaaagagaaa aaagagattg atgtacaaga attagtatta gatgatatta
      421 tgattttaaa aacaggtaat caaatttgtg cagattcaaa agttttaaat ggaaaattag
      481 aggtaaatga gtctttggtt acaggtgaat ctgatattat tataaaaaat aaaggcgatt
      541 ttttatattc tggaagtttt gtagtttctg gagaagcttt tgtaagagtt gaaaatatag
      601 gaaaagataa ctatgcaaat aagatagcta atgatgcaaa gatttctaaa aaacataatt
      661 ctcaacttag aaattcatta aatgcaatat taaaaattgt aggtataata ataattcctc
      721 taggaattat attgtttgca aaacaacatt ttggaaatgg agattctata gctacatctg
      781 tagtaggtac agtggcagct gtaaatggta tgataccaga gggattaaca ttacttacaa
      841 gcattgctct agctgtagga actataaaac ttgctagtca taaaacttta gtgcaagaac
      901 tgtactgtgt agagactctt gctagagttg atacactatg tcttgataag acaggtacaa
      961 ttacagaagg taaaatgcaa gttgatgata tagtaatgtt ggaagaagtg gatattaatg
     1021 aaataatggg gaatatatgt aattctctca aagatgataa tgcaacacta aatgcaataa
     1081 gagataagta taatgtttta gatacgtata aagctaagaa tttaatacca ttttcttcag
     1141 aaagaaaata cagtggggtt acctttgaaa ataaaggtac atatttatta ggcgcatttg
     1201 agtttatatt taaggaaaaa aataaaaaac ttaggataga agttgaaaga tattctaaaa
     1261 aaggaaacag agtaatagtt ttagctcata gtgattctta tatggaagat aataatattc
     1321 ctgaagatat aaagccacta gcttttatat tgatattgga taaaattaga gatgaagcta
     1381 aggaaacttt agaatttttt tacaatgaag gtgtagatat aaaaattata tctggtgaca
     1441 atcctataac agtaagtgag gtagctagaa aagcaggtct taaaacagct agtaatttta
     1501 ttgatgcaac aacacttaaa aatgatagtg atatatacga agcagttgat aaatacagtg
     1561 tttttggaag ggtaagccca caacagaaaa aacaaatgat tttagcatta aaaaaacaaa
     1621 atcatacagt agctatgact ggagatggtg taaatgatgt gctagcactt aaagaagcgg
     1681 attgtagcat tgccatggca tctggaagtg atgttactaa gaatgtgtct aatttagtct
     1741 tattagattc aaactttgca tcaatgccaa aggttgttat ggagggaaga aaagtaatta
     1801 ataacattca aagagcatct tctttgtttt tagtaaagac aattttttca ttcttcttgt
     1861 cgttattaac attattcttc ttcagtagac agtatccatt tgttcctata caattatcac
     1921 tcattggggc tgtaacagtg ggtataccat cattcttctt agctcttgaa gcaaacaaaa
     1981 gtagaatttc aggaaatttt ttactcaata tattaaaaaa tgctttacca ggagcactat
     2041 gtgtgacagt aaatgtaatt attgtttaca atatagttga atatttaggt tatagttcaa
     2101 gtgtattttc cacaatgtgt gctatattaa ctggtgtatg tggtctttta atattaagac
     2161 aacaatgttt gccatttaat aaagaaagat tatttttaat agtaagtatg actattgcat
     2221 ttgttattgg gattctattt ttaggtggat tattctcatt tgtagcattg agtagagaat
     2281 taattctcat aactatggca ttagcaatat taatgccaat tatgataaaa gttatgttat
     2341 ttgtcttaga tgagatatta gagacaattg ttccagattt aaattaattt taaaatgata
     2401 taaataagag cagttttagt tgaactgctc tttttagatg tgtttaattt tatgtaagaa
     2461 tatgtatttt tattaatcag ctatatatga gcaacagtag tcaacaactt cattaacctt
     2521 taagtcaaaa cttgaatcac taggaacatt aaatttttgc ccttctttat aagttacaaa
     2581 ctcattgctt ccaggcagtt ttacatccat acttccagca agcatctcca ttatttcttc
     2641 ttctctagta gaaaaagtgt attctcctgg caacattaat cctaaagttt ttctttctcc
     2701 attaggcagt attattactc tgctacttac cttaccatca aaataaacgt ttgccttctt
     2761 tactgcagta acattcttaa attcactcat tagataaaac ctccaaaatt atttgtacat
     2821 atatattata taaaaataat gtaaaaaatg ctatatattt acaaaaaaat ttagtattta
     2881 ttaaaaaaat taataagaat aaaaaagtga tgaaattgct taaattgata aaaaatataa
     2941 aataaattgc aaagtaagca agaaaagaat aagttgaaga gttatatatt ataaatatta
     3001 aaaattttag tattaataaa aaagatatta cattaatatg aaaatatagg agattagaag
     3061 tg
//
LOCUS       DAEMHW010000301         3043 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEMHW010000301 DAEMHW010000000
VERSION     DAEMHW010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.303"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      178..263
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            845..2959
                     /locus_tag="KPK67_003663"
     CDS             845..2959
                     /locus_tag="KPK67_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBG2460577.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDSQVPDGEKPSDGQVPDGEKPS
                     DGQIPDGEKPSDGQIPDGEKPSDSQTPDVEKPLDGQMPDGEKPLDEQTPEEEKPLEEE
                     IVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEEYVKADEQSEINQVFTSEDSFISL
                     WYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTLTFDYVIKGLVGASSKDDKYVIE
                     GEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQENQNEEAVKSILYAGSEFDGFGY
                     KQQFNLGEEENDIMTYSATQSAIWIILGQIDEQERLKQYQGSIDLCDKLIERAKTEEE
                     RAEYQKRKEVAINTKAYLEALLKAGREELKPNDTGKPSLSNGLTKINFEKNEDGTYET
                     EAVALVGYSGVVKLKLPDGVTAYDVDGNIIGTGEVEISTQQKFKLKSVGKPDAKANIS
                     AVSYDYIFPKAIQYYKAVLDLGQKDHSSLLPSSKQNLLSYTIEKKNGQEVNFNIGLPT
                     DDDDVVNPPVPPIDDTVINPPVPPTDDTNGHKPKPSPPIDDTVINPPVPPMDDTIINP
                     PVPPTEDAVLNPPVPPTDDTVLNPPVPPVSDTVEKTPELSRDDTIVKSPKTGDETQLM
                     SYVFISVIAICGLAYQCKIKRN"
BASE COUNT         1230 a          405 c          572 g          836 t
ORIGIN      
        1 tcaactatcc gatagagtaa aaggtttttg tgatatttga agatactcta tagtatcttt
       61 tttattataa aaactaattt ttgtctttta ttagataagt atctaggata ctaatttata
      121 ttttattgag ttgttaaaaa agtgcactta aaaagttaaa aaagaaaaat taaaaaacct
      181 atttatagaa actgtgaagt atatcttaaa cctgggcact taaaagatat atggagttag
      241 tagtgcaacc tgctataaat ataaatagga gtaactttta attgtcaaaa atatataaaa
      301 taaatacata aataagtaat taatagaaga tagatataaa tatatttttg aaatgctccc
      361 tgcattcata acgagtgtag agtggcagat atctgcccaa aactccttta attgctggaa
      421 gttcctaaag ctaactaaac cacaacataa tcatgaaata aggataagtg tgatagagtg
      481 tgcatttaat aagcacttga ggttacgaaa gtagaaaaaa ttagttagat agcataaggt
      541 taaatcctaa gtgccttgaa aatggataat cagcaggtaa gcttcgaaaa gaagaaactt
      601 caacgactag agagaaatct cgtacctaca agtgtgggga aatggggagt acctaattta
      661 tggttaggta atgatatagt ctgtgcttaa tagaaatatt aagaagttca taggagaact
      721 ggttaggagt aacgacccta attgaacaca ctaaagatgt attattgaat aagattcata
      781 taatatttaa taattttaat tatattaagt gaatacaata tatgttttta gtatcaggag
      841 aattttgaaa acaaaaatta aaaaatcaag tataatcagt ttagtaattg cttttagtat
      901 ggtatttact gcatttacac caatagtatc ttatgctgac gaagtgacta gcaatgatac
      961 aatacttaat ggagaagaac aatcaaatgg tcagacacca gatgtagaga aaccatcaga
     1021 cggtcaggta ccagatgggg agaaaccatc agacagtcaa gtaccagatg gggagaaacc
     1081 atcagacagt caagtaccag atggggagaa accatcagac ggtcaggtac cagatgggga
     1141 gaaaccatca gatggtcaga taccagatgg agagaaacca tcagatggtc agataccaga
     1201 tggagagaag ccatcagata gtcagacgcc agatgtagag aaaccattag atggtcagat
     1261 gccagatggg gagaaaccat tagatgagca aactccagaa gaagaaaaac cattggaaga
     1321 agaaatagtt atagaggaaa tgagtttaaa acaagatatt gataaaattc ttgatatgac
     1381 tttatctcaa ataaataaaa tagtatataa cttttgggaa gatgaagaat atgtaaaggc
     1441 agatgaacaa tcagaaatta accaagtttt tacttcagaa gactcattta ttagcttatg
     1501 gtatgataaa aaagcaaagg tgaaaaattc ttgtttacta aaagaaatag atggttcaga
     1561 tagaccttat catgatttaa gatttgatga tattacaaag actttaactt ttgactatgt
     1621 aataaagggt cttgtaggag caagctcaaa agacgacaaa tatgtaatag aaggtgaaga
     1681 tggagagcaa actgcatttt gttacaataa tcatttaaga ccaccaagta gtaatggaaa
     1741 gagtccatat ttaccagcag aagattttaa tggtcaagaa aaccaaaatg aagaagcagt
     1801 taaatctata ctatatgcag gttctgaatt tgatggattt ggatataagc aacaatttaa
     1861 tttaggcgaa gaagaaaatg acataatgac ttactcagct actcaaagtg caatttggat
     1921 tattcttgga caaatagatg aacaagaaag acttaagcag tatcaaggct ctatagacct
     1981 ttgtgataaa cttatagaaa gagcaaaaac tgaagaggaa agagctgagt atcagaagag
     2041 gaaagaagta gcgataaata caaaagctta tttagaagca ttgttaaaag caggtcgtga
     2101 agagttaaag ccaaatgata caggaaaacc tagtttgagt aatggtttaa ctaagattaa
     2161 ctttgaaaaa aatgaagatg gtacttatga aacagaagct gtagccttag taggttatag
     2221 tggtgtagta aagttaaaac taccagatgg agtaactgct tatgatgtag atggtaatat
     2281 aataggtaca ggcgaagtag aaatttcaac gcaacagaaa tttaaactta agagtgttgg
     2341 aaaaccagat gccaaagcta atatttctgc tgtatcttat gattatattt ttccaaaagc
     2401 tattcaatat tataaggcag tgcttgattt aggacaaaaa gaccattcat ctttattacc
     2461 atctagcaag caaaatttat taagttatac aatagagaaa aagaatggtc aagaagttaa
     2521 ctttaatata ggtttaccaa cagatgatga tgatgttgta aatccaccag tgccaccaat
     2581 agatgataca gtaataaatc caccggtgcc accaacagat gatacaaatg gacataagcc
     2641 aaaaccatca ccaccaatag atgatacagt aataaatcca ccagtaccac caatggatga
     2701 tacaataata aatccaccag taccaccaac ggaggatgca gtgttaaatc caccagtgcc
     2761 accaacagat gatacagtgt taaatccacc agtaccacca gtaagtgata cagtggaaaa
     2821 aactccagaa ctatcaagag atgatacaat agtaaaatca ccaaaaacag gtgatgaaac
     2881 tcagctaatg tcatatgtat ttattagtgt cattgcaatt tgtggattag cgtatcaatg
     2941 taaaataaaa agaaattaat ctagattatt attaagttta aaataaaaag tctcaaattg
     3001 atttataagt cattttgggg cttttttatg taaatataat atg
//
LOCUS       DAEMHW010000302         3021 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEMHW010000302 DAEMHW010000000
VERSION     DAEMHW010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.304"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            231..2366
                     /gene="rnr"
                     /locus_tag="KPK67_003664"
     CDS             231..2366
                     /gene="rnr"
                     /locus_tag="KPK67_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBG2460578.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1333 a          272 c          544 g          872 t
ORIGIN      
        1 acttaagtaa ttataatgat atttacaaat aatttataaa acatcataat ttgtttgaag
       61 ttagtatgac aaagcttaca ttaaatcaaa aatataaata agtaatttta acttataata
      121 ataataagaa aatatagaaa attatgtgat aagtaaaaaa tgttttgtct gaagaaatat
      181 aaaagacaat ataaataata atataatgaa aagtaaaaca ggagggaaaa atgataccag
      241 gacttaaaga aagtttgcta ggattaataa atgaaagtgc atataatcct ctaaaaaaag
      301 aagaattagc agaaatattt gatatacatt catcagaaat gccaatgttt tataattttt
      361 taaatgaatt agaagaagat ggttatatct gctttactaa aaaaggaaaa atagtatctc
      421 ccaatcaaat gggatacttt gttggaaaat ttgtatcaca caaaaagggt tttggatttg
      481 tagaatctga tgttgaatat acacaggatt tatttatctc aagtgacaat ataaatggtg
      541 ctatgcataa tgatagagtt atggcagaaa tagtagtacc tgcaactgaa gaaaagagag
      601 cagaaggaag aatagtaaaa gtaataaaga gagaaataac tagaatagta ggtactttcc
      661 aatcaagtaa aacttttgga tttgtaacac cagacaacaa gaaatttact aaagatatat
      721 atatacctaa aaaacatttt agtggagcag tagataatga taaggtagta tgtgaaatta
      781 cagtatggcc acaagaggat agaaaaccag aaggcaaaat tatagagata ctaggtcaaa
      841 aaggtgaaag aggcgtagaa atagattcta taataagaga acatggtctt ccagaagagt
      901 ttccaaagaa agtattgcaa gaagcagaag ctgtagctgt tgagattcct caagaagaga
      961 taaaaagaag aagagattta agagatttaa acatatttac tatagatgga gatgacgcaa
     1021 aagacttaga tgatgccata tcaatagaag tattacctaa tggaaacttc aaactaggag
     1081 ttcacatagc tgatgttaca cattatgtaa gagagaaaaa taaacttgac aaggaagcct
     1141 taaaaagagc tacatcagtt tatttagtag ataaagtaat tccaatgttg ccaaaaactc
     1201 tttcaaatgg agtatgtagc ttaaatccat ttgaagataa gctaactctt tctatattta
     1261 tggaaataga ccataaagga aatgtggtta aacatgaaat atgtgaatct gtaataaatt
     1321 caaaagctag aatgacttat acagaagtat ctgatatatt agaaaaggat gatgaaaaac
     1381 ttaaaaagac ttttgaacat gttgttgaag attttaaaaa tgctgaaatt ttagctagga
     1441 ttcttatgtc aagaagggaa aaaagaggag ctatagactt caatttccca gaggcaaaga
     1501 taattctaaa tggaaaaggc gaagttgtag atataaagcc atatgaaaga agaatatcta
     1561 ataagataat agaagaattt atgcttataa gtaatgagac aattgccgag catttttact
     1621 ggatgggaat accgtttgtg tatagaatac atgagacacc atctttggaa aaaatggaag
     1681 aattgagtaa gtttatatct acatttggat atataataaa aggtgataaa gaggaagtac
     1741 atccaaaagc attacaaggt ataatagaga aaataaaggg caaaaaagaa gaaggagcta
     1801 tcagtactat aatgcttcgt tctttaaaac aagctaagta ctcaccagaa tgtagtggac
     1861 attttggatt agcagctaaa ttctactgtc actttacttc accaataaga agatatcctg
     1921 accttcaaat tcatagaata ataaaagaat ctttaaataa taaaataagt ggaaaaagac
     1981 aagaacagct tactactata gtggattatg catctgttca atcatccgag aaagaaagaa
     2041 aggcagagtt agctgaaaga gatgtacatg atttctataa ggctctttat atgaaggata
     2101 aagttggtca agaatttgag ggtgttgtat ctagcgtaac ttcatttgga atatttgttg
     2161 agttagataa tactatagaa gggttgataa gacttgcaaa tatgaatgat gattactata
     2221 tttatgatga gagttcttat agtgttttag gagaaagaac taagaagtca tataagatag
     2281 gagaccttgt taagataaag gttgaaagtg ttaatgtaga ttttaaagaa attgactttg
     2341 agatagttga aaaaatagag gagtagataa aacagctaaa ataagagata acaagttgta
     2401 gtatttatga tatgtataaa tatatagtgc acaatactct ataaaataaa tgataaaaat
     2461 aggtaattaa gaaatatcta atggaaatag ttaactgaaa gataaataat taagaaatat
     2521 ttaatgaaag tagataattg aaaaataagt aattgagaaa tgtttaatag aagtggctaa
     2581 ttgaaaaata agtaattaag aaatgcttaa tagaagtggc taattgaaaa ataaataatt
     2641 aagaaatgtt taatggaagt ggctaattga aaaataagta attaagaaat gcttaataga
     2701 agtggctaat tgaaaaataa gtaattaaga aatgcttaat agaagtggct aattgaaaaa
     2761 taaataatta agaaatgttt aatagaagtg actaattgaa aaataagtaa ttaagaaatg
     2821 cttaatagaa gtggctaatt gaaaaataag taattgagaa acatttaata gaagaagcta
     2881 gctgaaagat aagtaattaa gagatattta atgtaaacag ataatttaaa aatacctaat
     2941 taagaataga tattaaaaaa tataatttca atataaaaat aagtgtatca tttaaaattt
     3001 aagatacact tattttttat t
//
LOCUS       DAEMHW010000303         2976 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.305, whole genome shotgun sequence.
ACCESSION   DAEMHW010000303 DAEMHW010000000
VERSION     DAEMHW010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.305"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1017
                     /locus_tag="KPK67_003665"
     CDS             <1..1017
                     /locus_tag="KPK67_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBG2460579.1"
                     /translation="IFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTSVPKVLYRTY
                     ESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVISSGLNCKSW
                     IRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTARNLKKYMPKS
                     EIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNTEYAVYNATK
                     PYSVNDIGYYDNDGYLIFEGRSDDIVNIGGFKVSSAKIENEVKKIPQIEDAIVLPYLD
                     TIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTSSEKIDRLAL
                     LKML"
     gene            1093..1968
                     /gene="dpsA"
                     /locus_tag="KPK67_003666"
     CDS             1093..1968
                     /gene="dpsA"
                     /locus_tag="KPK67_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBG2460580.1"
                     /translation="MSNQYDITVIGGNLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            1971..2561
                     /locus_tag="KPK67_003667"
     CDS             1971..2561
                     /locus_tag="KPK67_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBG2460581.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTVELALENKQLQPVIKSPHQ"
BASE COUNT         1137 a          374 c          472 g          993 t
ORIGIN      
        1 atttttaatc ctaaatttaa tatatatatg tatcaatata agaagcttat aaattattta
       61 gataaaagta tttgtatggg agctttatct tcaggttcta caagtgttcc aaaggtatta
      121 tatagaacat atgagagttg ggctggattt tttcctatac aaaatggtat ttttaatatc
      181 tctggggaat caatcttgtt tgttaatgga acacttagtt ttacagggaa tctaaattct
      241 ataatgtcag tattatatga aggaggtagt attgtaataa gttcaggtct taattgtaaa
      301 tcatggataa ggacaataaa acaatataat gtaactaaca tataccttat acctacaaaa
      361 cttcaacttt tggttaaaca cttaaaagaa ccagtactca aagttgtaag tatttttaca
      421 gggtctcaat tattatttga agatactgca agaaatctaa aaaaatatat gccaaagtct
      481 gaaattattt tatactatgg ggcaagtgaa ttaaattata ttacatattt atgctatgat
      541 gaactaattg aaaaacctct aagtgtaggc agaccatttc caggaataga tatatatatt
      601 agagaaggaa aaatttttgt gaatacagaa tatgcagtat ataatgcaac caaaccttat
      661 tctgtcaatg atattggata ttatgataat gatggatact taatatttga aggaagaagt
      721 gatgacattg taaatattgg aggatttaaa gttagttctg ctaagattga aaatgaagtt
      781 aaaaaaattc ctcaaattga agatgcaatt gtattgccgt acttagacac tataagagga
      841 aatcaaattg tattatttgt aataactata gacaaaatta cgaagaaaga tttgttgatt
      901 aaaatgaaag ataatctaat gaaaaatgaa atcccaaaga aaattatctt tttaaattca
      961 tttccatata catcttctga aaagatagat agattagcat tattaaaaat gttataagta
     1021 taatatttag taattttagt gaaccaatgt attgataaaa acatattgta tatagtaaag
     1081 gaggcttaca atatgtcaaa tcaatacgat ataactgtta ttgggggaaa cctgcgacaa
     1141 gtgtacatgg taaaaaaact aattaataaa gggtattctg tagtcactta tgggatatca
     1201 aatgaaatta taaatggaat cgttggaaaa tcaacttcac tagctgaagc actatccaaa
     1261 agttccatta ttttatgtcc aattccattt acaaagaatc aagttaatat tgaaaatacg
     1321 gatgattgtc ttgatgctac tattgataac ttgctaatta atttatctcc taatcaaata
     1381 ttatttggag gtaacatacc aactaaagtt tgtaaatttt gcgagcaaca ctctattcag
     1441 atttatgatt ttatgaaaat gaatgatgtt gctattttaa atgcagttgc tacagctgaa
     1501 ggggctatag cagaagctat taaaagaagt ataatcaata tacatcaaag taactgttta
     1561 gtattaggtt atggaagatg tgcacaaata cttgcaaaaa aattatacgc tttggatgca
     1621 aatgtatgtg ttggcgcaag aaataatgat gcaagaagta cggctaatgc atttggatat
     1681 tccagtattt ctttaagtca acttgatgaa aaacttttac aatttgatta tatttttaac
     1741 accataccag agtgtatttt aacgaaagaa aggttagaaa aagtatcaca agaagtaact
     1801 attatagata tagcttcttc tcctggagga gtagattatt ctgttgttaa agaagcaggg
     1861 attaatgcga ctctttgcct tgggcttcct ggtaaatatt caccaaagac atctggtgaa
     1921 attcttgtta acgctataga aaatattata aatgaaagaa gggattagaa atgagacttg
     1981 aaggacttac aattggagta ggatttactg gttcgttttg tacttatgat aaaatattta
     2041 tagagttaga gaatttggtt aaagaaggag ctaatgtaca cactatattt tcagatgtat
     2101 cacaaaatat cgattgtaga tttggaaatt ctgaagagtt tatgaaaaaa gcatatgaac
     2161 ttacaggaaa taaacctata gttacgattg aagaggcaga gccatttgga cctaaaggaa
     2221 ttgctgatat tataataata gctccttgta ctggaaatac tgctgcaaaa ttagcaaatg
     2281 gtattacaga ttcaccagta ttaatggcag caaaaggtca tcttcgtaat gataaaccac
     2341 tagtaatatc aatatcaacg aatgatgctc taagctttaa tttcaaaaat attggcattc
     2401 tccttaattc taaaaatata tattttgtac cttttggtca agataattgc aaagcaaagc
     2461 ctaattctat gattgcacat actgagctaa taattccaac tgtagaactt gctctagaga
     2521 acaaacagct acagcctgtt ataaaaagtc cacatcaata gaattggttc ttataatatt
     2581 atgtattaga taaaatacta gcaaagaggc tgtctcaaaa tagagagaag cctctttttc
     2641 ttgttgaaat gatgaaaata tatttaattt catacaaaaa aatgcctcat ggactaaaaa
     2701 gtctattttg agctaatctc cgtaattttt ggtatattaa aattatttac ttaatccaat
     2761 ttttaaatta gaatttatat cgttttgact cttcaaatat caatctaact gttgttgata
     2821 caaaagaaga ggacataatt aaaacaaata ccgatagtcc tctaataaac aaattatcta
     2881 cataataagt tactattcca atacctccaa tcgttaaaat tacacttata atcaaagaaa
     2941 aaacgctaat tgtttttaat tttttcatgc ttatcc
//
LOCUS       DAEMHW010000304         2964 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.306, whole genome shotgun sequence.
ACCESSION   DAEMHW010000304 DAEMHW010000000
VERSION     DAEMHW010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.306"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            156..809
                     /locus_tag="KPK67_003668"
     CDS             156..809
                     /locus_tag="KPK67_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG2460582.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            885..1094
                     /locus_tag="KPK67_003669"
     CDS             885..1094
                     /locus_tag="KPK67_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460583.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(1234..1800)
                     /locus_tag="KPK67_003670"
     CDS             complement(1234..1800)
                     /locus_tag="KPK67_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBG2460584.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            1961..2614
                     /locus_tag="KPK67_003671"
     CDS             1961..2614
                     /locus_tag="KPK67_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBG2460585.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQEISLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(2772..2948)
                     /locus_tag="KPK67_003672"
     CDS             complement(2772..2948)
                     /locus_tag="KPK67_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460586.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         1116 a          311 c          434 g         1103 t
ORIGIN      
        1 tgtttgggta aaagtataaa tagtaaaaaa gtagaagtaa tgtgtataaa aacgtaagtt
       61 acattctgta ataatagtta ttttataatt caacaaaaga atagaatggg tatatttgtg
      121 aaataataat ataaaataat cttaggagtg agcttatgaa gaaaaaatta ttatatataa
      181 atgtaaattc aaaacctgaa gacttatctt caagtaaaac tgtagcaaga aaatttataa
      241 ataaatttat ggaaaaaaat aaagattttg aggttgaaga gatagacctt tataaagaac
      301 atataccaag acttgagtat cagtactttg aaaaaagaaa tagcatagtt agtgaagaaa
      361 atgctaaaaa tttagacgat aaggatagaa aagagcttac aaagattaga aatctttgtg
      421 accaatttgt gagtgcaagt gtatatgtaa tagcagcacc tatgtggagt ttatcttttc
      481 cagcaccact taaagagtat atagattgta taattcaaga tggaaaaact atatcttttg
      541 aaggcaatga taaaccacaa ggaattttaa atgatattga tagaagtatg gtatatatac
      601 agtcttctgg tggacacata ccatgggtgt tgaagccagt tatgaataaa gggttaaatt
      661 atgtggaaag tattatgaaa tttataggaa ttaaaaaatt tgatgaattg ttagtagatg
      721 gaactggaac tagtgaagaa gaaagacaag ctgcaataga aaaagcatct gaaaaaatag
      781 atggtataat agatgggatg aaattttaat tataatattt tattaaaaat ataaaggaaa
      841 aacctctttt aatgtagaaa cttatattga aggaggcgag aatcatgcga gaagaaaaaa
      901 gtaatgaaaa gtatgattgt tattggtgta atcaagagaa taatttctgt gtagaaataa
      961 aagataatat agtcatgata gatgatggca ctggtacgtt aaaacaagcc gttttcatag
     1021 ggtataaaca aatccaaatt aatttaaatt gttcacattg tcaaaactta aatagaataa
     1081 aattaaattt gtagaatttt aaaatggaaa ttattgaagg aattaaaaat agaaagtgtt
     1141 aatatttaga ctgaaaatta tattttatac aataaaagtt tatttaaata acattgtatt
     1201 ttaatattta ttttcagtct tactttatta aaattatttt ttggttatgt ctggaaaata
     1261 tctgtctact aatttttctg aaatattttt taattggtca gccattgaag tcagacttaa
     1321 taaggctatc aataaatcat ctttattttc tggatttata ctaatatttt caaaattaga
     1381 ctctataaag tccggtatat tctttctttg attttctttt attaacatga atttttcgta
     1441 acatgaaact aaatcttttt cactgaaatt atccttttca tacacaccat gtaagattct
     1501 tttaatttct tgtattgtaa gtgtattttt catagaataa ataattatca tctgtaaaat
     1561 ttgttcttta gtatactttt ttcccttaac tggtttgatt aatccctctt tgctgtaatt
     1621 atttatcatg gttttagtaa gaattttatc actttcaaat cttttattag cagaaagctt
     1681 attgtcaatt agagaaataa tttgatccat atataagtca atagatggaa tatcgtttga
     1741 agttatatct gcattattta gtgtttctag tattatagta ttaagttcat tattcatcat
     1801 atgttacctc cataatatat ataatacact aaattagaaa gtaaaacaat attaaggctt
     1861 gtaaaaaaaa cataaagtgg tataattaaa tatataatat agtaatggaa actatatttg
     1921 tgaatctgat ttttaaaata cataattgta ggaggacgaa atgtatcaat tttttttaaa
     1981 gggtagagac ccaataagca gtttaactca ttttataggt gcttgtttat ctcttttagc
     2041 aactataatt ttagtttttc aatctgttac attacaggaa atttctctct tgatgatagt
     2101 atctgtatcg gtatttggac tttcactaat tgctttatat agtgcaagtt cgtattatca
     2161 ttttttaaag gggacaccag aacaagaatt gttttttaga aaagtagacc atgcaatgat
     2221 ttacgtattg attgctggct cttatacacc aatatgtctg aattttatgg aaaaaaaaga
     2281 aggcataata tttgttacag caatatggat agttgcattt attggtataa taataaaaat
     2341 attttggatg gatgcaccta gatggttatc taccagtata tatttactta tggggtgggc
     2401 aattgttttt gatataaatg cattcaattc aattcctaag gattgcctta gactattaat
     2461 tatggaaggt gtatcttact ctataggtgc aattatttat ataattaaaa agcctaatat
     2521 aagtcctgag tttggatttc atgaaatatt tcatattttt attatgatag gttctttatt
     2581 tcacttttta gcagttttat tgtatgtttt atagttttaa aagatttgat taattgatac
     2641 taaaattcta caatgaataa aatggaactg ccttatatag attatatctt agacaataag
     2701 acagttcctt attataaata ttaaattcta cttataataa aattttgata aaaatagtcc
     2761 attcaaaatg cctattccat aattccttta atgtactttt catttacgac atatagtttg
     2821 aatttatgta ctataattat atcctctaaa ttctctgaaa cactaacaat tctgacacat
     2881 ttgtttctaa tttctacagg taaatctaaa tcatttgtta aattcacata gtcatcatat
     2941 ttatacataa aatcatccct ttac
//
LOCUS       DAEMHW010000305         2962 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEMHW010000305 DAEMHW010000000
VERSION     DAEMHW010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.307"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            83..451
                     /locus_tag="KPK67_003673"
     CDS             83..451
                     /locus_tag="KPK67_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG2460587.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            454..825
                     /locus_tag="KPK67_003674"
     CDS             454..825
                     /locus_tag="KPK67_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBG2460588.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(969..1424)
                     /locus_tag="KPK67_003675"
     CDS             complement(969..1424)
                     /locus_tag="KPK67_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460589.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1463..2728)
                     /locus_tag="KPK67_003676"
     CDS             complement(1463..2728)
                     /locus_tag="KPK67_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBG2460590.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1101 a          410 c          407 g         1044 t
ORIGIN      
        1 aaatttaaaa ataatggttg aaaaatataa ttaagagtac tataatttaa ttaagtaatt
       61 acttaatcaa aacgaggtac ttatggaaaa cttggttaaa atatttaaag cattgagtga
      121 tgaaactaga cttaacatac ttatattagt atccaagaga aatatatgtc aaaaggggat
      181 ttctaaatat ctagggattt cagattctgc tgtttcacaa catataaaaa tattaaaaga
      241 tgttgggata ataacaggat ataaagaagg atactatgtt ttatatcata ttaataaaga
      301 atcatttaat atatgtgtag attttataaa ttctatgtta agtaattcta gtgaaagctt
      361 tatagatgta tttgatgtaa atacaataca tctaggatgc agtaaagaat gtaaatctat
      421 aaaaaaatgt tgtaagagaa gggagaaata ataatgaaag tatgtatacc agtagaggag
      481 aataaaggat tggatagtaa accatatgga cattttggtt ctgctccaat ttttgttgtg
      541 tgtgatttag aaagtggaga agttaaatct ttagataatg gagatttaga tcatgaacat
      601 ggaaaatgtc aaccattaaa agcattgtca gggacagctg tagatgcagt agttgttgga
      661 ggaataggac aaggagctat aattaaatta aatggaatgg gaataaaagt ttacagagca
      721 gaaggtgata ctataagtgc taatttagac ttacttaaaa atggaaaatt agtagagttt
      781 ccatcagacc atacttgctc acatgacgga tgtggacatc attaaaaaaa acagctgatg
      841 aaatatatac ttcatcagct attttttctt ttaataaata cgattttatt cttttaaaac
      901 caaaatttag atttttatgt tttttgtagt atccttaata atatttcaag tttacaagtt
      961 tactactttt agattttttt tgtatgtttt tctatatagt caatatcatt taatatgtca
     1021 ttgagttctt tattaagtgt ttttaaatca tcaccatcta cattgtagtt taaaaatagt
     1081 tcctctacat tatcaatgat agaattgact tttctagtaa cttcaacaaa tgttttaaag
     1141 ttactactat ttttagcatt tagaaatttt tttaaatcct ttgatataaa tgatactatt
     1201 tcatattcat cattaaatag acctctacca ataggatatg gttcaattct ctgtaaaaaa
     1261 tattggtctt tagcattaat attataagta taagatattt cccaaccgtc tcctaaatca
     1321 aacttgataa aatataatgt actacttact tttgatattc ttgtggcacc aagctgtctt
     1381 aaaagaactt caatcgaatc accttgaaca gtattaatat tcatatatac acatcccctt
     1441 tagtattatt atactatatt ttttaaaatt ttataattaa ataaacagta gatataataa
     1501 tagatataat cataagtgga aaacctattt tgaaatactc cttaaaagag ataggatgac
     1561 cgtgtttatt acctatacct gctagtacaa cattagcaga tgctcctatt agtgttccat
     1621 ttcctccaag acaagctcca agagatgttg cccaccatag tggcattaca tcaatacctt
     1681 cagcttgcat agtcaaaata agaggaatta gtgttgcaac aaatggaata ttatctaaga
     1741 aagaagagac tatagctgac aaccataata tcaacaacat tgtaaataca agatgtcctt
     1801 ctgtaagtcc aatcaatgct tgagccaatt tgtttattat cccaacttct actaatccac
     1861 caactactac aaataatccc ataaagaata aaattgtaga ccattcaata cctgccataa
     1921 tttcatctac atcttgtttt cctattaaaa gcattataga tgctcctgtc agtgctacaa
     1981 cagaagaatc tatgtggatt gtactgtgaa acataaaacc aaaaagtatg aatgctatta
     2041 atataaggct ttttataagt agaggtttat ctttaacaga ttttttttca tctagtttta
     2101 atattgcatt tttagctctt tcattcacaa caagttcttt tccataaata aatctaaagc
     2161 atattatagt tatagcaagt attactataa cagcaggacc taaatttatt acaaaatcca
     2221 taaaactaag attggcagca ctaccaatca taatatttgg agggtctcct ataagagtag
     2281 ctgttcctcc tatatttgaa gctaaaatct gtgttattaa aaatggaact ggattaagtc
     2341 ctaatatttg tgtaataact atagtcattg gtccaataag taagacggtt gtaacattat
     2401 ctaatacagc tgataaaata gctgttataa tagcaaagca aatcattatc ttccatgggt
     2461 cacctttagc tttctttgca gtccagatag ctatatattc aaataacccc gagtttttga
     2521 caacagctac aaatagcatc ataccaacta atacaccaat agtattgaaa tctatatggc
     2581 ttaatccttc atcaagtgta agtatattga atattatcat taatagtgca ccaccaacag
     2641 ctgctacgga tctgtttacc ttttctgtaa taatagctgc cataactatt aaaaatatac
     2701 atatagctat tatttgttga aattccataa tataagtacc tccatttgta ttattttgtg
     2761 cctaatgcta gtactatata tagtattact tttttattgt tttttcaatg gatgtatgaa
     2821 agtatattat ttaacaaagt ttttgaaaaa ttgacagaat ttaaagaaaa tttttgttat
     2881 ttaaataaat tttaaaatta ttccattgat aataatatta aacgacaaac gaaaaaatat
     2941 accctttaaa ctagaattta tt
//
LOCUS       DAEMHW010000306         2944 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.308, whole genome shotgun sequence.
ACCESSION   DAEMHW010000306 DAEMHW010000000
VERSION     DAEMHW010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.308"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1609
                     /gene="aspD"
                     /locus_tag="KPK67_003677"
     CDS             <1..1609
                     /gene="aspD"
                     /locus_tag="KPK67_003677"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBG2460591.1"
                     /translation="EIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAGRGNPNWT
                     CSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDMPGAYLAR
                     DIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQEMKYDISG
                     KYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPNLPRYDFK
                     VVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDETSCNNLID
                     VIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWRLGTFALH
                     KKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNHTAGLSTP
                     QQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAAYYTQFDI
                     EEWAKLNYGEDLFKFISKTASPVDFLYKLANDYSVVLLSGNGFYGPEWSIRISLANLY
                     DEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1730..2725
                     /locus_tag="KPK67_003678"
     CDS             1730..2725
                     /locus_tag="KPK67_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBG2460592.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILSLGKTDNLDEIKDFFEKGIYC"
BASE COUNT         1069 a          399 c          494 g          982 t
ORIGIN      
        1 agaaatcaaa aacatatatg gaaaaataag cccttttgaa tttaaggaca aattaataga
       61 tattgcaaaa tacactgcaa aagaaaataa cagagaatta cttgatgcag gtcgtggaaa
      121 tccaaactgg acttgttcaa ctgctagaga agcctttttt acttttggtc atttcgctat
      181 tactgaaact cgttctaatt gggatttagg tcatttggct ggaatgcctc aaaaaaaggg
      241 tattaaagaa agatttttta aatttataaa tgaaaatata gatatgcctg gagcatattt
      301 agctagagat attataaact ttggtataaa tgaacttgga tttgatggag atgaatttgt
      361 tcatgaacta gctgatggaa ttattggtga taactatcca cttccagata gaatgttacc
      421 tcatatggaa aaaatagtac acgattacct agttcaagaa atgaaatatg atatatcagg
      481 taaatatggt gatgtagaaa tttttgctgt tgaaggtgct acagctgcta tgtgctatat
      541 atttgattca ttaatggcaa atgaacttct aaaaaaagga gatacaatag ccttaatgac
      601 tccaatattt acaccttatc ttgaaatacc taaccttcct cgttacgact ttaaagtggt
      661 aaatataaat gctaatgaag ttgatgaaaa aggtgctcac acatggcaat atacaaaaaa
      721 agaattagag aaacttcgtg acaaatctat taaagcttta ttcgttgtta atcctaataa
      781 tcctgcttct atagctatgg atgaaacatc ttgtaataat ctaatagatg taattgaaaa
      841 ttataataaa gatttaatga taatatcaga tgatgtatat ggaacttttg tagaagattt
      901 ttcatcctta atgtcaaaac taccttacaa cactgttgga gtatattctt attcaaaata
      961 ttttggagtt acaggatgga gacttggtac atttgccctt cataagaaaa atgtttttga
     1021 taagaaaata aatgatttaa ctggagaatt aaaaaaatca gtagataagc gttacagtga
     1081 tatgagtctt aacccttcta gcctttcatt tatggaaaga gttgttgctg atagcagact
     1141 tgttgctctc aaccatactg ctggtctttc tactccacag caagtacaaa tggcgttttt
     1201 ctctgctttt gcattgattg acaaagtaga tgcctacaaa aaactcaata tgagtatctg
     1261 ccatagacgt caaaaactat tatttgaagc tttagagctt ccaattaatg aaaacaaaaa
     1321 caatgctgca tattacactc aatttgatat tgaagaatgg gctaaattaa actacggaga
     1381 agacttattt aaatttataa gtaaaactgc atctccagta gattttcttt ataaattagc
     1441 taatgactat tcagtagttt tacttagtgg aaatggattc tatggccctg aatggtcaat
     1501 tagaatatct ctagcaaatc tatatgatga agcttatact aagataggaa aagccataag
     1561 agagatatta aatggttata tcgttgaatg gcaaaaaatt aagaaataat tatattacac
     1621 tgaaatatct aaattatatg aataaaagag tttatgtaga ccttaactac tttttttgat
     1681 ataatcctat cggaattata aattttattt atgaaaggca gagaatagta tgagtattaa
     1741 gaaaaaagtt gccatagttt ttactggtgg aactatatct atgacagtag atgataaagt
     1801 cggagcagct ataccaactt tatcaggtga acaaatacta tcaatggtta ccaacattga
     1861 taaagttgca gatgttgaag tttttaattt tgatgaaatt ccaggacctc acataacacc
     1921 ttatagaatg atggaattga aaaattatgt aaatgattta ttagcaagaa aagatattac
     1981 aggtgttgta ataacacatg gtacagatag tttagaggaa acagcttatt ttctagacct
     2041 tacaattgac aacatcaaac ctgtaatagt cacaggtgct atgagaagta gttctgaact
     2101 tggatatgat ggttcaagta atttatctgc atcagtttgt actgcagtat ccaaagatgc
     2161 tatgggaaaa ggagtccttg tagttttaaa caatgaagta ttattagctt ctgaagctac
     2221 taaaacaaat actctttctc taaatacatt taaatctcta actagtggtc cattaggaat
     2281 aattgactgc aacgaacttg tccttacaag agatattgta aatagaacaa ttatagatac
     2341 tgataaggta gaatctaaag ttgccttatt taaagcatat gtaggagaaa atgctgactt
     2401 tataagattt gctgttgata gtggttataa aggtatagtt attgaggcaa tgggtagagg
     2461 taatattcct cctcaaatgc tttctggagt tgaatatgct agagaaaaag gacttcctgt
     2521 agttatagtt tctagatgtc attcaggtag agtattcgat agttatggtt actttggttc
     2581 tggtagagat ttaaaaaatc ttggttgtat atttggtggg gacttaccag gtcaaaaagc
     2641 tagaataaaa cttatcttat cacttggaaa aactgataac cttgatgaaa tcaaagactt
     2701 ctttgaaaaa ggaatctatt gttaattcta ataacttcta tttaattatt tattgatatt
     2761 tcaacatttt taaatcaaaa tctaatactt atgtcagtta aatgtcagca aaatatttta
     2821 tccacaattt ttacacaagt tttccacaac ttatccccaa tattatccac aagaaaaaag
     2881 gtagcactta tattagtagc taccttttat ttattaatct tcatctttcc agctttttat
     2941 ccaa
//
LOCUS       DAEMHW010000307         2940 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEMHW010000307 DAEMHW010000000
VERSION     DAEMHW010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.309"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(55..828)
                     /locus_tag="KPK67_003679"
     CDS             complement(55..828)
                     /locus_tag="KPK67_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460593.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDILDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(834..1721)
                     /locus_tag="KPK67_003680"
     CDS             complement(834..1721)
                     /locus_tag="KPK67_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460594.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(1735..2757)
                     /locus_tag="KPK67_003681"
     CDS             complement(1735..2757)
                     /locus_tag="KPK67_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460595.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVHLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
BASE COUNT         1028 a          480 c          262 g         1170 t
ORIGIN      
        1 tttatcttat ttttaattaa actaaaatat aaataaaatt actattattt ttagttaaaa
       61 ccttttgctt gatgtaaaat tttcatatgt tattttagtt aacactatca ttaatataat
      121 tgagattact atagaaaaaa ctatcaaaat acttggtaca ttatacttaa ccatttgttg
      181 gctcatcata atccaatttg gagtcactga cataaatttt atagctttat ctggtataaa
      241 acctacacta aagtatggaa ttgccattat caatccacct agtataaatg gaattacagc
      301 cgatttaaat atagatgaaa gtgttaagcc tatttgtgca aatataaaag aagcaaaaat
      361 agtagtcaat ataacaatta tatacatttg tattactgta agatttaaat tactatttcc
      421 aaagtaccat aagttatgaa tcggttcatt taaattttta aaatcaaaaa aactaaaacc
      481 tctaattcct actaacaata taaaagaaat attaataata gataaaatta atccattagc
      541 tactagtttt gataatacta ctgatttttt tcccttttta gttgtattta ttaaacctgt
      601 catattagaa tcatattctt taatgtaatt tccagaagtt attataaaca ttatcaaacc
      661 tattagaggt attgagcctt ctatcttgta aaatatatct gcataagtat ctaaaatatc
      721 aaactttcta atcagaaaaa atccaaaaaa tattaaaaac agccaaatta tccaaacact
      781 ctttgatttt aatattttta tcatttcagc tttaactaaa tacatcattt ctactacacc
      841 tcccttgcat cttccaaatc aaaataaaac atgtatacat cttcgaattt tgcttgaact
      901 ggttgtatat tatcctcgct tggaatatcc tctgaaacaa ttctaagtac tactgaatca
      961 atatcacttt gaatacttac aaccttatat ttagattgta tatcatcaac ttcacattca
     1021 tttttaactt gaacattata tactttattt tccatatgac ttaatatgtc tttgtgtgtt
     1081 cctttcatta ggagtttgcc atctttaatc attattgttt cctttgcaac tgattctata
     1141 tcggaaatta tatgagtaga aagtattatt atcttatctc tagacatttt tgctattaaa
     1201 ttcctaaatc tagctctttc ttgtgggtca agacctgctg ttggctcatc aaggactatt
     1261 atcttagggt cgtttagtaa aacttgtgct atcccaactc tcctcttcat acctcctgaa
     1321 aattttccta tagacttatt tctcacatca taaagaccta ctaaatctaa taattctcct
     1381 atcttactat ttaaatattt tccccttaaa ccttttaaag ctcctatata ctgtaaaaat
     1441 tcttttgctg taaaattttt ataaactcca aattcttgtg gcaaatatcc taaaatattt
     1501 ctataatcag catccatttt aaatatatcc tcaccattat aaagtatctg accttcatca
     1561 ggttttatca acgttgttat ttgtttcatt aatgtagatt ttcctgctcc atttggtcct
     1621 agaagaccat aaactccatt ttccagagtt actgtaatct tgtcgtttgc tactttttta
     1681 ccatatatct ttgaaagatt ttctatttct aatttattca tcttaactcc ccttttatct
     1741 acttatataa tctgatttta ttactaaaaa acataatgac atagcaccta tcaatattgc
     1801 catcaaaagt cctattgcta tattgacaat gtctatattc actttaaata tcataatatc
     1861 acctagataa tttcctatta ttttattaaa accaaatatc aaatcataat aatttacatt
     1921 tgatacaact gaaagtaatt ttttaggtaa tatatggatt accgacatta tcttagttat
     1981 agatataata aatataaaaa aacttattga agatattgaa gacttactta taaaagaccc
     2041 aaatatggct atcatggccc atccttcaaa catcaaaagc tctatgccta tatttaatat
     2101 tacatacttt gttatactta aattagtttt caatgcaact acattgtcta ctattctaaa
     2161 agattctaag tctccattaa caggtactcc ttgttgcata caagttataa taaatattcc
     2221 cattaaatga acagaataaa ttactaatgg aactaataaa gcaacaaata ttttagactt
     2281 taatgctttt ttcttctctt ttgatgatag tatgatatct tttaccgaag taacgacttc
     2341 atctacgtaa atattagata taattataga tataatgatt aacattccta catttataag
     2401 tgaatttgta actctatagt taaatacttt ccaaaaacta atatccttat attcattcac
     2461 acttagatta tcaagttttt tctttgacat taattcaatt tttttagaaa attcacctgt
     2521 atctgtttga tttgaaaact gctttaaaac attaatctta ttttgaaatt tctcattacc
     2581 tacgtcgata ccacttctcg tatcttctat acgtcctttt tctttatcta tataataact
     2641 ttctgtttct aaaacaggcg taataaagat actaatcatt agtattaaca ctaatatccc
     2701 tagagatata aacatactct tagatttagc caattttttt atttcccaat ttaccaatat
     2761 atttctcctc ctaaatcatt ttaattacac atatatatta aaacttaaat cttatataaa
     2821 tataatacta accttaaata aagcttaaat tattttataa tttataggtg tgtttattat
     2881 tggagttaca tttattattt ttctgggaaa ttatacattt tcaattattt ttattatttt
//
LOCUS       DAEMHW010000308         2904 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEMHW010000308 DAEMHW010000000
VERSION     DAEMHW010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.310"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            68..141
                     /locus_tag="KPK67_003682"
     tRNA            68..141
                     /locus_tag="KPK67_003682"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Gly,seq:tcc)
     gene            150..266
                     /gene="rrf"
                     /locus_tag="KPK67_003683"
     rRNA            150..266
                     /gene="rrf"
                     /locus_tag="KPK67_003683"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            540..2558
                     /locus_tag="KPK67_003684"
     CDS             540..2558
                     /locus_tag="KPK67_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2460596.1"
                     /translation="MRRNTKLLTTGILSMAIVTPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT         1169 a          355 c          493 g          887 t
ORIGIN      
        1 gatatatatg tagtttttag agtgctaact ctaaaaaatt agttttacta atttataaaa
       61 atttaatgcg ggtgtagctc aatggtagag ttctggcctt ccaagccagc tgtgagggtt
      121 cgatcccctt cacccgctcc aaaagattat gtggttacaa tagcagagag gatacacctg
      181 ttcccattcc gaacacagaa gttaagctct ctagcgctga tggtacttgg tgggaaactg
      241 cctgggagag taggacgtag ccacgtaatc tttttttgtt gtattaaatt taataaacct
      301 acaattgtta tatgttttta tttgtctttt atagattttt ttggcaataa atgatatagt
      361 gaagataatt aaatatattc aatatattta atttttttat tttttgtttt tataattttt
      421 ttagaagttt attttttata taattattta taattttggg taaaaacact ttgatatatt
      481 ttcaaaagat aaaagattaa aaaacaaggt agaaatttat gttaagtaag gagattaata
      541 tgagaagaaa tacaaaatta ttaacaacag gaattctttc aatggcaatc gtcacaccta
      601 caatggcatt tgctactgaa tctaatgcta tggaaaataa cgctgattta aatataaact
      661 tagagaaaaa aagtatcgtt ttaggtagta cctcaaaagt cagtgttaag tttaaagaaa
      721 aaccagatgc agatagcatt acattaaagt ataaatgcta tgatatgcca ttggatacaa
      781 ctttaaacta caatcaatca actgagtcat atgaaggaac tatcaattat aataaagacc
      841 cagaatatct aaatgtttgg gaactacaag ggataacaat aaacagcaaa aataatccta
      901 aaactttaaa caaacaagaa ctagaaaaga tgggattaaa tttaaaagac tataatgtaa
      961 cgcaggaatg tataattgaa gatataactt ctagaaaaga tgtaaataaa tatttaagaa
     1021 aaacttctgc acctattaca gaacttacag gaagtgatag atacgaaaca gcagttaaaa
     1081 taagtaaaga aggctggaaa aatggttcag ataaggtagt tataataaat ggggatgtaa
     1141 gtatagatgg cattatatca actccactag caactacata taatgcgcca atacttttgg
     1201 ttgaaaaaaa caatgtacct aatagtgtaa aatcagaatt aaagcgttta aatcctagag
     1261 atgtaattat aattggagat gagaatgcta tttctaaaac tactgctaat caaattaaat
     1321 cgactgtaaa tgctagtcaa acacgtctaa agggctctaa tagatatgag acatctttat
     1381 tgatagcaaa ggaaatagat aaaaatcatg atgtggaaaa agtatacata acaaatgcta
     1441 atggcggaga agtggatgca cttactatag cagcaaaagc aggtcaagac aagcaaccaa
     1501 ttatattaac tgataaaaat agtattacag acaacacata taaatggtta aagagtgagg
     1561 atttacaaaa tgcttatttt ataggtggac ctcaaatgat atcaacaaat gttataaata
     1621 aggtaaatga tataactaaa gataatgtta ctaataatag agtatatgga gcagatagac
     1681 acgaaacaaa tgcaaacgta ataaaaaaat tctatacaga tgatgagtta gaggctgttt
     1741 tagtagctaa atcagatgta cttgttgatg ctttagcagc aggtccattg gctgccaact
     1801 taaaatctcc aatacttata acaccaaaga cgtatgtatc tgcataccat aaagataatt
     1861 tagaagctaa atcagctaat aaagtatata aaataggagg aggattgact tctaaggtaa
     1921 tgaactctat agcatcatca ttatctaaac acaatacaac tccaacagaa ccaggaaata
     1981 gtgggggtaa gacagttatg attgacccag gacatggtgg ttcggacact ggaacaacag
     2041 gtaaaccatt agggggtatt aaagaaaaag actatacatt aaatacttca cttgcgacaa
     2101 ctgaatattt acgttcaaag ggattcaatg taataatgac aagagataca gataagactt
     2161 tatctcttgg aaatagaact gctttatcta actctttgag accagattta tttacgagta
     2221 tacactataa tgcatctgat acaactggga atggtgtaga agtattttat aagcttaaag
     2281 ataaagatgg aggaactact aagactgtag ctacaaatat attaaataga attttagaga
     2341 aatttaatct aaaaaataga ggagcaaaaa caagaacact atcaactgac cctacaaaag
     2401 attatttata tgttttaaga aataatgata tgccagctgt acttgtagaa tgtgcatttt
     2461 tagataatga gaaagatatg agtttactta atacatcaaa taaagtaaaa gaaatgggta
     2521 cacaaatagg taaaggcata gaagattcat taaaatagtc tacaatgaga gcatagaaaa
     2581 atttaaaata aattaatctg caattttaaa tttaaactaa agctataaga taagaatgta
     2641 attataatag taataaagac tgtatataca ttgtgcatat aagtgcgaaa tgaggtatat
     2701 acagtctttt tatgattaat tgtaaaagga tttatattat atgaagaatt tacatatctt
     2761 aaggctacag ttaattgtca agagttggag tattcatttg taatacacaa taaaagctta
     2821 attactatat tttattgtgt gtttgtgtgt tttttgtgga tttttatgtc aataaatgat
     2881 atagtataga taattaaatg tatt
//
LOCUS       DAEMHW010000309         2902 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.311, whole genome shotgun sequence.
ACCESSION   DAEMHW010000309 DAEMHW010000000
VERSION     DAEMHW010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.311"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(768..1397)
                     /locus_tag="KPK67_003685"
     CDS             complement(768..1397)
                     /locus_tag="KPK67_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBG2460597.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEIIVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(1476..2438)
                     /locus_tag="KPK67_003686"
     CDS             complement(1476..2438)
                     /locus_tag="KPK67_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG2460598.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
BASE COUNT         1000 a          441 c          290 g         1171 t
ORIGIN      
        1 aataaattgc aatagaatat tgaaaatatt caataatatc ttcataaata tattttataa
       61 aagattttat aataaacatt acaatacagt actaaattag tttatttcat ctatcatctc
      121 aatcatatca tttactaact attattctaa tgagcatata acattgaaat ttataattat
      181 acaacctaaa tgcctcccaa tgtaccctca aaaacatcat ttcctgtaac tgaagtataa
      241 ttggtatatt ttatcactga aataaaaaaa cactagtcaa taaagactag tattttcaag
      301 tattttatca ttcttttttt aattattgga gacgaatcat cagaataatg cccataattt
      361 atctatatgc aaattctaaa tttaaaataa agtttcagat gaatttacta ttctatgcta
      421 tgatacaaca gaaaaaactg aaatctatga gtggtagctt catataatcc tagttcctac
      481 agtaatcaaa actgaggttt attctatatg taactaatga taaattttat tataggattt
      541 attagctcta tttgtgttac tataagtagt aattatatat atgataattt tttaacactt
      601 ttctattgct atatagaaag tggtttagaa tttaaattta aataaaattt aaattcacca
      661 aaaagaaatc tatattagaa accactactc tattccacca gattgtaatt ttttgttctt
      721 tatatcacaa tgctatacca cattaaattg aaaaataaat tttaaattta tttgttacaa
      781 ttcattaatt caaaaaaata ttttgagtca tgagttttgt aaagatttag ccatgaattt
      841 aatgcttctg attcaaaaac atctaaaagt ttcaatactt gaaacgtaaa ttccaacgga
      901 gcaattcctg aagccgtaac taaattttca tcactaacta cagattccgt tttataatat
      961 ttttctccag agtaatttgg tattaccatt ttaagaaact ctaagtcatt acttgtatga
     1021 tttctaaaat ctaatagccc ttttttagct aggcctaatg tagctccaca tatggctgca
     1081 actatagaat cttgtattaa agcttcttta actttttcta atattatctc atgattcata
     1141 gataaccaag tatttccccc tggcaaaatt aataaatcct tactttccat attaaactca
     1201 tcaactataa tttcaggtaa aacttttaac ccacccattg ttataatagg atttttatca
     1261 attcctacag taattacttt tagtggcttt aatccttttt taaaatatct tcctgtattt
     1321 aattcagcag ttaaatatgc cacttcccaa tctgacatag tatcaaatac atacatatat
     1381 acttttctat ttttcatctt ttttactcct tatgtaatta atttatatat ctaattataa
     1441 aataacttaa ctgacatatt cagtcagtta agctattaaa tttgataata atcaattaat
     1501 tctagtaaat cttcaattat tttcttccta aggcttagtg gttcaattac ttgtattgtc
     1561 tttctataag gtaaaagtat atttggtatg tacttgcata ttacttcctc ttcaagcata
     1621 aatattattt tattaggtgt tctttctttc aagtagtgcc ctaaaaacca atgttggctt
     1681 aaactatcta aagaatctat actcccttct acaactaatt gaattatatt ttgttcatct
     1741 tttatttcag gtataattcc ttttataaaa aattcacttg ctgaaaattc tgtcggtctg
     1801 ttaaatgtat ttgatgtttc tatgatttca ctaatcctat ccactctgaa actacgtacc
     1861 tcatttctca tattacaaaa tgcaactacg taccaattat tattccagtg aacaattcca
     1921 tatggattta taattctata ttttgatttt gctttacgac ctgcatgata gttgatttct
     1981 acagatagct cctttaatat actattctcc aattttttaa gtactgattc tatagacact
     2041 ttcccaattg gttttattac ttcaagggct cttaagtggt ttgttaaaat atcttcttgt
     2101 ttttgatttg aatacatttt taactttaat atcgatttat ctaatgcttc gtctaagaag
     2161 tatccttctt ttttagcaaa tagtgctgca tgaagtagag atttttgctc ttctatatca
     2221 aaaaaaagag gtgctttagt aaaattattt aaaaggctat acccaccatt atgacctgta
     2281 tctgaaatta taggtactcc actcgcagaa agtacatcaa tatatcggta cactgttctt
     2341 atatttattt ctaatttttc agctatctgt tttgctgtaa tttttttgtc agagtttaac
     2401 atccacagta tagacatcat attgtcaatt ttagccatgt agtttcacct ctttctattt
     2461 gttatataaa gttattatat aactttttga ttgatttttt acaaaaaaac attagtaaac
     2521 tgctaataat ttatactaag atttatttca aatatttata gatagtcttt ttctatagtt
     2581 aagttatcca ttaaattaca tagtagtata tctattttag ttgatatatt ttcagctacc
     2641 tcccttgaat attattacaa taagtctaaa tcttctttct tccataaata tattatgagg
     2701 aaagactatc atctttaatg gtttaatagt ataaaagata ttaatttaaa aaaatctatt
     2761 tttatatcag atgggtattg ttttttataa aactgaaaaa atcacctaat agaatctatg
     2821 attttttaac ataacctcca taaattagtt ccaattatca cttcaaattc aaatttttat
     2881 ctgaagaata tacaatcata tt
//
LOCUS       DAEMHW010000310         2863 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEMHW010000310 DAEMHW010000000
VERSION     DAEMHW010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.312"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1..1110
                     /locus_tag="KPK67_003687"
     CDS             1..1110
                     /locus_tag="KPK67_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG2460599.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1487..2671)
                     /locus_tag="KPK67_003688"
     CDS             complement(1487..2671)
                     /locus_tag="KPK67_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460600.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1082 a          437 c          396 g          948 t
ORIGIN      
        1 atggatttca aaagcttagg gattagtgaa aatacaataa atattctaaa aaaatcagga
       61 ataacaacac ctacacctat acaaaaagaa agtattaaac taataaaaga aggaaaagat
      121 gtaatagcag aagctcaaac tggaacagga aaaacactag cttttttact tccgatattt
      181 gaaaatatat cattagatat aaatgatata caagtactta tactttctcc tacaagagaa
      241 ttagctattc aaataacaga agaagctatg aaacttaaag aatctaaaga tgtaagtatt
      301 cttgcagcat atggaggaaa agatattggt tctcaaataa agaaactaaa aggaaatatt
      361 catatgatta tagctacacc aggtaggctt ttagaccatc taaatagaaa aacaatagac
      421 cttagtaagt taaagacttt tgttttagat gaagcagacc aaatgttgct tatgggattt
      481 aaaaatgaag tggaagctat tttaaaagag acttcaaaca aaaagcaaac actatgcttt
      541 tctgcaacta taaattctca agtaaaaaaa ttagcttata gatacacaaa aaatcctgtt
      601 gtagtttcta ttcaaaaaga agaaatcact cttaataata taaaacaaga agtagtagaa
      661 actacagata gaaaaaaatt agatgcctta tgtaaggtac tagatgaaga taatcctttt
      721 atggcaataa tattttgtag aactaagaga agggttgaca atcttgaaga agctcttgct
      781 ataagaggat ataattgtca aaaacttcat agtgatattg ctcaatcaaa acgagaaaga
      841 ataatgaaat catttagaaa tttagatata caatacttaa tagctacaga tgtagcttct
      901 cgtggtcttg atatatctgg agtaagtcat atatataatt acgatttacc agaaacacca
      961 gaagactaca tccaccgtat tggtagaact ggtagagctg gtgaagaagg ctatacatgt
     1021 gcttttattg accctaaaaa tgaacgaatg ttaagtgaaa tagagacggc tatagaatct
     1081 aaaattagta gaagaattat agaattataa tttatagatt gcttaaaata ccaatgccac
     1141 acaaacactg taatatatag aaagctacag aaaacatgta tcaatctata ctttgctgtt
     1201 atactatttt atattaaaga aaaaagaggc tatccaaagt aggattagtc tcttttattg
     1261 taaaaataat gaaaatatat ttgacttcat ataaaaaaga gtgccttata aactaaaagt
     1321 ccattttgag acaccctctt tttatctttt attcttagtt ttatattgct tgtttatagt
     1381 tgtttaatat tagattttgc aaataatctt aataaacatg cagatatatt atctatagta
     1441 ttcttattta tagtattctc tttagcaata ttttttgcta ctacatctaa tttacatttt
     1501 catttaattc cgctggccat ttagcttctg gattttcctt tgcaaacttc ttattcttat
     1561 aatgtcctag tgtaaggaat ggaattacta tagtgaatag accaagataa ccacaatatc
     1621 catatccata tttaacaata ttacttaatc ctgtaagtga tataagcatt gacactacca
     1681 ttattgcaca agctgtaaat acccttcttt tcattcttga agaaaggaaa gaaagtgctt
     1741 tatggtcttc aaatctattt acaagaccaa atatagtagt tactcctgta gacattaaac
     1801 aaaggaataa tgcaacatta taaattatgt atatagtatg atttccagat tgacctgcaa
     1861 cataaagagt agggagtgtt gtttgcccag cagcaataat ttctctatac catcccatta
     1921 gcatagttac agcaagtcct aatgcaattg cattcataac aaatgaaatt atcattgcct
     1981 tagtagcttc ttttgagttc ttaagaagtg gtcttgaaca tcctgcaagt gtaggtataa
     2041 caaccgattg aaaacctgca taaataaaag tattaagtat tggcttgata tagccaggtg
     2101 tgaaactcgt tctatttgat aatatttcag ttatttctgg tactttagct tttataccaa
     2161 caataaaaat agtaagagta catactagaa tagctattgt cattgctgta gctgctttag
     2221 atacaagcat agcaccaaat atagtaaaga caagaagtac aactccaata ataaatacag
     2281 ctacaaaata atttactcct atactttgaa atactgctgc tgcacctgct ataacagcac
     2341 ttacagctat taaaaccatt agatagtaat atatctccca tattatttca agttttggat
     2401 atggtctcca aagctcacaa aataaatcct tataattcct acaattgtta ttattataca
     2461 ttatgataac ttctctcatg gtagcagtaa gtataatcat tgctaaaatt gccataagtg
     2521 gtgctgtcca tccatactgc acatagtact gagtttcttg attccctgta gcaaatcctc
     2581 ctcctgcatg tgaactaaaa agaacgcttg caacaccaaa aatagctaac atagagcccg
     2641 ttacttttgc tttctttaca cttttttgca tgaaatcatc ctccctaaaa tgatattaaa
     2701 agtttttatt ttaacacttt attgataaaa caataacatt tttgctcatt aaattcaact
     2761 atatttttcc aatgacttta cattttcaac ttatttctac ctttatatta aatatttttt
     2821 atatatttat tttatttttt aaatagtatt caaattcact ttg
//
LOCUS       DAEMHW010000311         2850 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.313, whole genome shotgun sequence.
ACCESSION   DAEMHW010000311 DAEMHW010000000
VERSION     DAEMHW010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.313"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            114..323
                     /locus_tag="KPK67_003689"
     CDS             114..323
                     /locus_tag="KPK67_003689"
                     /inference="COORDINATES: protein motif:HMM:NF024835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2460601.1"
                     /translation="MKSKRNPYLKFKIFLMERGIKQSELCKILGKSASAINQNINGTG
                     GDFSVEDLRIISQTYNISIDEFFIN"
     gene            386..1276
                     /locus_tag="KPK67_003690"
     CDS             386..1276
                     /locus_tag="KPK67_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBG2460602.1"
                     /translation="MNEVVNAENINEIEVIGITEVDGMKFHDIEGGFGECKKAMLVKE
                     IAEIHNKELKHINELINKNRKRFQNDIDIIDLLGVGLNDTKIKKFGFTQQAINSYRGS
                     KNKGLLTGIYLLSERGYAKLLKILEDDKAWEQYEKIVDGYFNMRKSLKENNKFNSSKP
                     KDIKDESEIKYMNAQARLKNARAREAKIYLELADKVDIKEYKQIMYSKTTELLSGETL
                     IPLPKMEKKTYSATEIGKILGISANKVGGLANAYNLKTDEFGIKVWDKAKHSNKQVPN
                     FRYYENVIPILEKALSSFEN"
     gene            1678..1932
                     /locus_tag="KPK67_003691"
     CDS             1678..1932
                     /locus_tag="KPK67_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460603.1"
                     /translation="MNDRERIFYKIVDTLDTIADIKIADQNKVEKEVGMCIGEDNYIT
                     YCIDENLIKFYIKDEEELVIDKDSPLLYMLDTLFYEVHEE"
     gene            1984..2172
                     /locus_tag="KPK67_003692"
     CDS             1984..2172
                     /locus_tag="KPK67_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460604.1"
                     /translation="MGKKSEYTVTTVGEPNLDALGETLAKILTEKFGVLITHTYIENY
                     YVDKERGGDVDGAIESEL"
     gene            2147..2374
                     /locus_tag="KPK67_003693"
     CDS             2147..2374
                     /locus_tag="KPK67_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460605.1"
                     /translation="MELLKVNFNLEKLKIGNVVSVSSKRFGFNINCIVVVATEEELNL
                     AYYSKDKGCIEYQALIPEDIRYGDYILERLG"
     gene            2474..2791
                     /locus_tag="KPK67_003694"
     CDS             2474..2791
                     /locus_tag="KPK67_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460606.1"
                     /translation="MESLKIVKQYVEGQLNLSSLEIDKNKETYEILKNKSSRDMLDDI
                     NLNDALREVTVNERLKIFAESLLELLDTQIKIKESEESEDYKRLCMYLDEFGRDRPID
                     VQI"
BASE COUNT         1238 a          222 c          550 g          840 t
ORIGIN      
        1 gtttcattgt caatgattta atttaaaaaa aataaagaaa gttattgaca atgaaacttg
       61 caataatata taatttaggt atcaaatatg aaacaaagaa gggggtgaaa aacttgaaaa
      121 gtaaacgtaa cccatatctc aaatttaaga tttttttaat ggaaagagga attaaacaat
      181 ctgagttatg taaaatactt ggaaagtcag catctgctat aaatcaaaat ataaatggaa
      241 ctggaggtga ttttagtgtt gaagatttaa gaattatatc acagacatat aatataagta
      301 tagatgaatt ttttattaat taaaaagttt cttatatgaa acaagagctg tttaaaaaat
      361 acaaaaaact agggaggaag aaattatgaa cgaagttgta aatgcagaaa atataaatga
      421 aatagaagtg ataggaatta cagaagttga tggtatgaaa ttccatgata tcgaaggagg
      481 ttttggagaa tgcaaaaaag caatgttagt taaagaaata gctgaaatac ataataaaga
      541 gttaaagcat ataaatgagc ttataaataa gaatagaaaa agatttcaaa atgacataga
      601 tataattgat ttgttaggtg tcggtttgaa cgacaccaaa ataaagaagt tcggatttac
      661 tcaacaagca attaattctt atagaggttc aaaaaataag ggtctattga cagggatata
      721 tttgttatct gaaaggggtt atgcaaagtt acttaaaata ctagaagatg ataaagcatg
      781 ggagcaatat gagaaaatag ttgatggata ttttaatatg aggaagagtt taaaagaaaa
      841 taataaattt aatagtagca aacctaaaga tattaaggat gaatcagaaa taaaatatat
      901 gaatgctcaa gcaagattaa aaaatgcaag ggcaagagaa gcaaaaatat atttagagtt
      961 agctgacaaa gtagatataa aagaatataa acagattatg tattcaaaaa ctactgaatt
     1021 actttctgga gaaacattaa taccacttcc taaaatggag aaaaaaactt attctgctac
     1081 agagataggt aagatattag gaatttcagc caataaggtt ggaggattag caaatgctta
     1141 taatttaaaa acagatgagt tcggaattaa agtttgggat aaggctaaac attcaaataa
     1201 acaagtacct aattttagat attatgagaa tgtaatacca attctagaaa aagctttaag
     1261 tagttttgaa aattaaattc agaagttgct aagtacaact ttagaaactt aaatttaagg
     1321 aggaataaga aaatgaaaga aaataacaga atagtaagtg ttggaactga aagtgagata
     1381 gatttaacag ttaaggaatt gaatatgata gatgatttca agaaaattat aaatggtgtt
     1441 aacaatgaaa tagctaatga ttattctatt ttagaaatgg atagagatat tttagagaaa
     1501 attgcattaa gtacagcttt gaactataaa aaaatgaatt ttgagtattc agaaatagat
     1561 gaaaaactat ttaatgattt tacttttgag tttatgctaa agttttttgg tgatatagaa
     1621 gaggatgaat taatcaaata tctcgtttat ataggagagg aagaggaaag ttatgaattg
     1681 aatgatagag aaaggatatt ttataaaata gtagatactc ttgatacaat agcagatata
     1741 aaaatagcag accaaaataa ggtagaaaaa gaagtaggta tgtgtatcgg tgaagataat
     1801 tatataactt attgcataga tgaaaattta ataaaattct acattaaaga tgaagaagaa
     1861 ttagttatag ataaggatag tcctttgtta tatatgctag acacgttatt ttatgaagta
     1921 catgaagagt aaaaattgca atgattaaat acggaatatt ttgaaaatag aggggtgatt
     1981 taaatgggta aaaaaagtga atacacagta acaacagttg gtgaaccaaa tttagatgca
     2041 ttaggtgaaa ctttagctaa aattcttaca gagaaatttg gggtactaat aactcataca
     2101 tacattgaga actattatgt tgataaagag agagggggtg atgtagatgg agctattgaa
     2161 agtgaacttt aatctagaga aattaaaaat aggaaatgta gtgagtgtaa gttctaagag
     2221 gtttgggttc aatattaatt gtattgtagt agtagcaact gaggaggaac taaatttagc
     2281 ttattatagc aaagataagg gatgtattga gtatcaagca ttgataccag aggatattag
     2341 atatggtgat tatattcttg aaagattagg ttaggaggaa ataaaatggc agctttaata
     2401 atagtaggtt tatttgcaat atgtttagta ggattagttc aaaatagaga ttaattaaaa
     2461 agggggttta attatggaaa gtttaaaaat agtaaaacaa tatgttgaag gacagttaaa
     2521 tttatctagt ttagaaatag acaaaaataa ggagacttat gagattctca aaaataaatc
     2581 tagcagagat atgttagatg acattaattt aaatgatgca ttaagagaag taacagtaaa
     2641 cgaaagatta aaaatatttg ctgagtcatt actagagttg ttagatacac aaatcaaaat
     2701 aaaagagagt gaagaaagtg aggactataa aagactttgt atgtacttag acgaatttgg
     2761 aagagataga cctatagatg tccagatata aaaaaagagc cattaggagt ggctcgattc
     2821 agaaaatatt taaaaataaa aatatgacta
//
LOCUS       DAEMHW010000312         2832 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.314, whole genome shotgun sequence.
ACCESSION   DAEMHW010000312 DAEMHW010000000
VERSION     DAEMHW010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.314"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            107..1030
                     /locus_tag="KPK67_003695"
     CDS             107..1030
                     /locus_tag="KPK67_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBG2460607.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1005..1832
                     /gene="panB"
                     /locus_tag="KPK67_003696"
     CDS             1005..1832
                     /gene="panB"
                     /locus_tag="KPK67_003696"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBG2460608.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGTFPQEKHSFSINESELEKLY"
     gene            1851..2699
                     /locus_tag="KPK67_003697"
     CDS             1851..2699
                     /locus_tag="KPK67_003697"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBG2460609.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNNYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1149 a          337 c          453 g          893 t
ORIGIN      
        1 aatgttttta attattttgt cttatgtaac tcatatcctc aggaatgaga ttagaaatta
       61 agatgttgta tcacaaagtg gaggtgatac tattaaaata ggctttattg gttctggaaa
      121 agttggaaca tctttggcaa attatttttt atcaaaaggt tattgtgttt caggctttta
      181 tggaaggaat caattatctt tattagaatc aacaaattta acaaaaacaa agatttacag
      241 caatttgaaa gatataattt atgaaaacga tatattattt attacaacat cagatgattc
      301 tattgaaatt atagacaaaa aactttccaa atttaattta aaacataaat acatttgcca
      361 cacaagtggc tctttaaaat ctagtatatt gttctgttca aaaaaagcag gtgcattaat
      421 ttattctatc catcctatat ttgcattttc caataaaaat accaacataa aaaaaatgaa
      481 agacatttgt ttttccatag aaggtgataa tgaacaagat gatttagtta ttcaaaattt
      541 catagatgat ttgggaaacc aattttttat aagagataag gatacttcat ccacttatca
      601 tttagctaat gttttagttt caaatttagt attatcttta ctagatatag gagtaagtta
      661 ctttattaaa ttaggtttat ctgaagagga atctttaaaa gctatcagtc ctttagtaaa
      721 aaaaaatata gaaaatatac ttgataatgg ctttactaaa gctcttactg gtcctgtttc
      781 tcgtggagat ataacaccaa taaggaaaca tctatctgta ttggataaaa aagatgagga
      841 tatttacaaa atactatcct taaacctact aaaaataatt gccttaggaa atgacaattc
      901 attaaaaggt ataaaaaata ttactataga aaatgcaatc gaaaatttaa taagtaaatc
      961 tgaaaaatac aaagaaattt acaaactttt aggaggaaaa aacgatgaaa aataccatac
     1021 agacttttaa gaatgctaaa tatgaaggaa aaaaactatc tatgcttact gcttatgact
     1081 actcaattgc aaaaataatg gatgagtgtg atataaatgg tattttaata ggagattcat
     1141 taggaatggt tataaaaggt gaagaaaaca ctctatctgt taccatagac gaaattatat
     1201 atcatacaaa agctgtaaaa aatggtgtaa aaaatgcctt aatcgtaagt gatatgcctt
     1261 ttctatctta ccatgtttct atagaagatg cagttaaaaa cgctggtaga cttataaaag
     1321 aaggaggagc tcatgctgta aagttagaag gtggctcaaa cgttataaag caaatagaaa
     1381 gcattgtaaa tgctcaaata cctgtaatgg ggcatttagg attaactcct caatcagtaa
     1441 attcatttgg tgggtttaag gttcaaggaa ataccagtga aacagcaaga caacttattg
     1501 aagatgctaa attaatagaa aaagcaggtg cattctctat tgtccttgaa ggagtgccta
     1561 cgaaaatagc tgagatggta actaattcta tttcaattcc tacaataggt ataggggcag
     1621 gaattaactg tgatggacaa attttagttt atcaagatat gttgggaatg tttggagatt
     1681 ttgttcctaa gtttgtaaag cagtatgcaa atataggaga tataatgaaa gattcaatta
     1741 aaaattacat cctagaagtt aatacaggta catttccaca agaaaaacac agcttttcaa
     1801 taaatgaatc tgagttagaa aaattatact aatattaagg atggtacaaa atgcttgtaa
     1861 aagaaattaa attactaaga aatataatca aagattggag aaaacatggc tattctatag
     1921 ggcttgttac aacaatggga tttttacatg aaggacacca aagtcttata aaaaaagctg
     1981 taaaagaaaa tgataaagta gtagtgagtg tatttgtaaa tccaactcaa tttggaccaa
     2041 atgaagattt taataactat ccaagagata tagataaaga ttttaaatat tgtatggata
     2101 gtggagcaac agtagtcttt aacccatcac cagaagaaat gtatttaaaa ggtaattgta
     2161 ctaccattaa tgtttctgga cttacagatt ttctttgtgg agctaaaaga cctgttcatt
     2221 ttggaggtgt ctgcctagtt gtttcaaagt ttctaaatat agtaacacct gataaagctt
     2281 attttggaga gaaggatgcc caacaattag ctgtaattaa aagaatggtc aaggatttaa
     2341 acatagatac agaaataata ggctgtccta taattcgtga aaatgatgga ttagcaaaaa
     2401 gttcgagaaa tacatatctc tctgaggaag aacgaaaatc tgctctaata ttaaataaga
     2461 gcttaagttt agcaaaagaa gaattagtaa aaggcaatct aaatccagag aatataaaag
     2521 aattaataac agctaaaata aattctgaac acttagcaaa aatagactat gttgaaattg
     2581 tagatagtga gacattacaa cctgttaagc aaatagaaca ttctatactt gtggcaattg
     2641 ctgtgtttat aggaaaaacc agactaattg ataattttac atttgagtta aatatatagt
     2701 ctataatttt atataatata tctaaaaaat aatagaatac ctaaaaagta ataaattacc
     2761 taaaaaataa taaattgctt agaaaataat agattacaat aactaaaatt ttaaaattaa
     2821 attccttaaa ag
//
LOCUS       DAEMHW010000313         2763 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEMHW010000313 DAEMHW010000000
VERSION     DAEMHW010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.315"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(352..1248)
                     /locus_tag="KPK67_003698"
     CDS             complement(352..1248)
                     /locus_tag="KPK67_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG2460610.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYIDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1389..>2763
                     /locus_tag="KPK67_003699"
     CDS             1389..>2763
                     /locus_tag="KPK67_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG2460611.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCELSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFRFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLI"
BASE COUNT          941 a          383 c          322 g         1117 t
ORIGIN      
        1 cttttaattc tttaggatgg atttaaattt tctataaaaa tcatactatt ttagctgttt
       61 acttgcttat ttgatagttt tatttctttg attaaatatc tttcagaatc taagccagat
      121 gtctcaaaaa aattgattta taatctaatc attttttctg ctattcttat ttttccagtt
      181 ttagtttcat attaaaatat acttttcata gacactgcat tctttcatac tcattttcac
      241 atttaactat acaagtagtc ttactcattt atgtactata ctttattaat atttattaag
      301 aaaaactcaa agatttaaaa atatatattt tctataaagt atatttatga attatttcat
      361 atcttctttt aaaataacat tactaacttc tctatgttca tttctatact tagttggaga
      421 tatattataa ttccttttaa ataagcgact aaaataattt atgctatcaa agccacatct
      481 catagcaatt tctgtcatat ttaaatctgt ttcctttaaa taatatatag ctttatcaag
      541 cctgattcca tttataaaat cgattgtagt cttacctgtt atttgcttaa atgttctaca
      601 aaagtgatgt gcactcaagt tcacacgact agcaagttct tttaatgtaa ttttatctat
      661 atatttttct tctatcaaat caaagataca ttcaaatttt tctaaagaat actttctttt
      721 tataaattca tcttctgaat atattttatc aatatgactt cttaataaga gtactattaa
      781 ttgatagata ttagctttta tagataactc atatccaact tctttattgt gatattcatc
      841 aagtatagtt gtaatacatt ttaaaatcag agaatcattt tgaattaaat tattaaatac
      901 aatatgattt aaagccaatg gtgctaagta cttagtttgt aacaaatcga cttgattact
      961 aaaaaggaat gtgggttcaa tacgaatact atagaatttt aaattatctg ataagctttc
     1021 taaataatgt aattcattac tatttgctat tacaatattt ccaggcacta ccttaaaatt
     1081 atccaatcca cattcaagat atgctatacc atcaataaaa taatatattt gtagatgctc
     1141 atgccaatgt cttttaaagt ttgctccttt tttaatgtca tcctttaaaa aaatatctat
     1201 aaagttttct tgacctttcc tgttaaaata tgtattttta tccttcatta ttcccccctc
     1261 atcaaaatag tgctaattat aagcaatata gttattgtat tcattatttc atatcgctat
     1321 tatataaaat gtaactatca ttgtaaacat taacataatg cagtcattat aataaggagg
     1381 taataaaaat gacaaactct tttaaaaaag atgataatag aaaaaaaatc acagttttat
     1441 ttgtagtcat tgccatgact tttatggcaa cattagattc tagtattata aatgttgctc
     1501 tcccagttct tgcaagcaaa ctaaatgtat ctctagcatc tattgaatgg gtaatagcaa
     1561 gttactcaat tattatttgt tcaacactat tattttttgg aagacttgga gatattattg
     1621 gtaaatcaag agtatttcaa gtaggtacta ttctctttac atcagcttca ttactatgtg
     1681 aattaagtaa ttctttaact ttacttatag tttgccgctt tattcaagga ataggcgctt
     1741 ctgcctacat ggcaaacaat catggaatta ttacagaact atttccaaaa gaaagcagag
     1801 gaaaagccct agggattcta gttactgcag ttgctattgg aaatatggtt ggaccatctg
     1861 tgggaggatt tattttgtca atatttgatt ggaatgttat attttttata aatattccaa
     1921 tcggtcttat tgtaatcttt ttaaatacaa aatttcttcc taatagtaaa aaatcttcag
     1981 aaaatatgga taaaactggt gctatacttc aatttttagg aacaacatta ttttttagtg
     2041 ctctaattag tgctcaacaa acaggattac taaatccata tatattaatt gcactattat
     2101 tatcaattat ttttattata ttatttttga tattagaaaa aaaacatcct caaccgcttc
     2161 ttgaccttga aatttttaga aattttaaat tttcattgaa tttaatttgt gctcttacat
     2221 cattcatttg tattgcatca tcaagtattt taattccatt ttatttacaa agtactatga
     2281 aacttccccc tattcaagca ggtttattta tgatactatc acctttgatt ttagccattt
     2341 tttcacctat atttgggaat atatcagata aaattaagtc agaaaaaatt attttaattg
     2401 gtcttctcgt tatgagtttt agattctttc taatgtcaag attgaaagaa agttctgccc
     2461 ttattttatt tgtaatttat atatcgataa tatcaatagg acaagctata tttcaacctg
     2521 ctaacaatgc tttgataatg tcaagctgtt ctagaagtaa gctaggagtt gtaggaagca
     2581 taaattccct tgtaagaaat ttaggacaag ttataggtat tactatttct acaacacttt
     2641 tatataattt tatgagtatt aaagctggat atagggtcaa tgattatgtg attaataatg
     2701 ataaaatatt tgtatttgga atgagaaatg tttatattat tgttacactt gtttgcttaa
     2761 tag
//
LOCUS       DAEMHW010000314         2713 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.316, whole genome shotgun sequence.
ACCESSION   DAEMHW010000314 DAEMHW010000000
VERSION     DAEMHW010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.316"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..559)
                     /locus_tag="KPK67_003700"
     CDS             complement(<1..559)
                     /locus_tag="KPK67_003700"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBG2460612.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHDLMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKN"
     gene            complement(721..1938)
                     /locus_tag="KPK67_003701"
     CDS             complement(721..1938)
                     /locus_tag="KPK67_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBG2460613.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(2129..2542)
                     /locus_tag="KPK67_003702"
     CDS             complement(2129..2542)
                     /locus_tag="KPK67_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG2460614.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT          939 a          413 c          306 g         1055 t
ORIGIN      
        1 catttttaac atcttcaata agtttattcc aaatatcaat attgtgtgga ttttctttat
       61 ccatttttat attttgaata agattgttta attttatttg ccattcatct ttaaatataa
      121 attcatgtcc attatgtatt ataccattct ggtaaattgt agactcaaaa gttgaactag
      181 tcgaaaaaag agttactttt tgtgagatag taggtaattt tttaactgtt tcttctacaa
      241 tatttaatat tggtatatta atagactctt ttaattcttt aaagtatata tgtgctgaat
      301 tgcatggcat tgcaataaaa tttacaccag ttgattcaag cttttgtaat ccatcaatta
      361 tagtttcttt cataaggtca tgatttattg gacggtcaat ataaaatggg gtaggtaatg
      421 aatatatcat catctttgga aattcatcat catattttgc tccatattga gattgacact
      481 catcaataat taaatcaaca aaaggggctg ttgaccttgg acccattcca gccaaaatac
      541 caattaattt ttcattcata aaaattatct cctttatata taaatatatt tatgtataaa
      601 ataaaaatct ataaaagcat aaaattatat tttataatag attatattgt acacttatat
      661 aatataaata taaagtgatt ttttagatta tatattttaa tatactttct tatataagaa
      721 ttattttaaa tcaagattat ctagtatttt taaaaatatt tttacagcag tatccaagtc
      781 atctacagaa acactttcat taggattatg acttacgccc ccagcgcatc ttataaatac
      841 cataccaata tcagttatat tatccatttc ctgagcatca tggccagcac cactataaat
      901 ataaaaagga ttaagattta aatctataaa acttttttct atgatttttg ttattttatt
      961 agaacatggt acaggaacat tttcaaaagc aagttcactt gtataattaa gttttctatt
     1021 ttcacatata tgtgatattt cattaaatat cttttctaca gaattatcaa gaatttcttg
     1081 tgaacaagag cgtacatcga gtgtaaattc agctctttca ggtaccacat ttgatgaaga
     1141 tggatagaaa ttcatctttc ctacagtagc aaccaaatca gcagtcgttt tagctacttt
     1201 ttcgatagct aatacacatt cacaggcaca acatccagca tcaagtctca tattcattgg
     1261 aagtgtacca gcatgtccag acttaccatt tactgatatc ttaaaacttt taaatcctgt
     1321 aatacttgat acaattccag caggaagaga ttcattttct aaaacaggac cctgctcaat
     1381 atgaaattct aaatatgcat ctacatcttt tctaggatgc aaatttgtta agttttttgt
     1441 attaaatcca aactttgtta cagcttcttc aagtgtaatt ccattttcat caacagattt
     1501 aagaagttcc tctttaaaag taccagcaaa aactttactt cccattaaag aagtttcaaa
     1561 tctagttcct tcctcttcag cgaaagcaat tagcttaata ttgaaaggat aagacctatt
     1621 attgtgtttt gcttcttcaa gaccaactag aggcactatt atacctaaca taccatcaaa
     1681 tatacctcca cactttacac tatcttgatg agatgcaact actaatgtgg gagcatttgg
     1741 aaagcttgat ttatatgtac ctataacgtt tccaatgtca tcaatttcaa tatcaagatt
     1801 taaaccttcc atccatgaac taattaaatc aatagatttt ttatgttctt gagtcaagat
     1861 aagtcttgta agaccatttg ttgttgaact tatttttcct aatgtttgta gataatccat
     1921 acattttttt cctaacatat ttacctccta aaaatataat ttttatttaa gctaagatta
     1981 gcttttatac ttataatttt tgaactttca aataattttg tatgtatagt aaaatactat
     2041 tttagtatta ataatgatat aaaaacataa agtctgtaga cgaagaacag aagttttaag
     2101 ttaaaaatat gaaatatgat ttttattatt atagtatttt tctataataa tatctctttc
     2161 cagttagagg ataattttca agtgaaaata ctttatggta tccaaatttc tcataaaatt
     2221 taggtgcttg aaaactgaat gtatctaaaa aaacatattt acaacctcgt tctttagcaa
     2281 ttttttcagc cttatataaa agttgacttc cagtaccttt atatctaagc ttctcactaa
     2341 cccataagta ttttatagaa agccaattac catgagtaac acctataaaa cctgcaacaa
     2401 ttttacctgc tttatctcga atatatattc ctaaatccac tgggttttta tcttcaagtc
     2461 tagcaagatt atattttaat aatccttgaa aaatagtatc ttcatcttgt ttttctattt
     2521 tttctgtaat ttctaattcc atatttgaac tttctccttg tataatcttt tgtatattgg
     2581 tattttttaa taaaatcagt agaaagaata tagtttaaca ttattctaat aggcatttat
     2641 gctaatgtca aactaataat acttattctt aattttatat tgttgatatt aaattttttt
     2701 aaataaaaat gta
//
LOCUS       DAEMHW010000315         2709 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEMHW010000315 DAEMHW010000000
VERSION     DAEMHW010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.317"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            321..1364
                     /locus_tag="KPK67_003703"
     CDS             321..1364
                     /locus_tag="KPK67_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460615.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKEVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            1475..2617
                     /locus_tag="KPK67_003704"
     CDS             1475..2617
                     /locus_tag="KPK67_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG2460616.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         1222 a          221 c          480 g          786 t
ORIGIN      
        1 tcaaatgata atatagtcta tttatgtaaa tgttatattg tatcctaagt ataatagtaa
       61 gttttttgat agtggtgtag aaagtaaaaa tctactagcc taaattgatg tatggattag
      121 tttaggaaca gtaattgcat atcttaagtt tgatgtcatg ttgggctact agaaataggt
      181 catctttttc acttgaatat cttttcttta aacatgggga gtatcatatc aaaaacatat
      241 gaaaattaag tgatttatga gaacagtaat attttatatg atagtataga atagtaataa
      301 agtattagga ggaacattga gtgttaaaaa agtggtttgg tattgtgaaa aaaagacaaa
      361 aaagtgagtc agtaaaagaa gaaggtgaag taatattaaa aaacgaaaaa atattatctg
      421 aagaaaagtt gatagatgaa gaaggagttg tagttaatat tgataatgaa atattaacta
      481 aagtagaagt agtaaatgat gacaatgaaa tagaagaaaa aataatagaa gaagattggt
      541 taattagtga aaataccata aaattagatg ataaagaagc aattattaat gataaaaaca
      601 tagaattatg taaaaaagaa gttcaagttg aaagtgaaaa aataggttta aataagtttg
      661 aaggacttga ccaaaatgaa aattataatc tagaaaaaaa tgttattgaa gaaaaagaag
      721 taagtgaatg tttgacagaa gaagatttag agtacataaa agaaattaaa ataaaaagag
      781 gaaaaagtat aaaagctata aacttgtata ctaaagaaga gtgggttttt gacactcata
      841 tacagtgtag taaaaaactc aaagttccat tagggtacat aagagaaaat ttaaaatatg
      901 gatatatgga ttactttgga gatgcaataa attatttaag tgaagtatta aatatagatg
      961 aatactgcaa aagtgaatgg agctatctcg ataatagcaa atctccatct gagatattta
     1021 atattctaaa caataaaata tttagtataa ggctttctaa tgaaaaaaga aatgaaattt
     1081 tgacaaatga taaaattgaa gcattaaaaa tgaattatag atttgaatgt attgatgaag
     1141 aatatgatga atattttaaa aaatataagt ctataatcaa aagaggtgga aagaaaaaag
     1201 ttgaattagt aaataaaaaa ggtgacattt tagaaatatt taagtcttta gaagaatgtg
     1261 ccatttattt gcaaaaggaa aagaatgaag ttatacaaat gttaaaatat ggagatacaa
     1321 aagtaggaag aaactttata aggtatagtt tgagaagtat ttaatttcat tttaaattca
     1381 taagagtttc atttctgatt cacacattta atattatttt aatgtaaaat aataaaggtg
     1441 aagtattgca caattaaaat taaggagggt attaatgtct gaagaaaaaa atccaaaaga
     1501 aaatacatta gatgaatcta aaaattcaga taatgtaaat tctaaaaaag atgatgtgtc
     1561 agatgaaaaa aatactgata acaaagttaa aaaaagggaa ataaagctaa acaacaaaac
     1621 acttaaagct attgtagcaa ttgttgcagt aggagctata tgtttaggtg gaggctatgt
     1681 ttttggcaaa gaagctggaa gaaaattgcc tgctacttca aaacactata atagcagtga
     1741 ggtagtagct actattgggg ataaaaaaat aacaggtgaa atgttaagaa agagaatgga
     1801 gccattattt tatttaaatg caaaaactac tatgactgat gacgaaatac gttcatatga
     1861 atcaaatatg attgaaacta tgattacatc tgatatatta tcctctgaag cagcaaaaga
     1921 gaaaataagt ataactaatg accaactaga tgctcagtat aataatttaa tgagtagtat
     1981 agaatcagca atgggaatga ctaaagaaga gtttttaaaa cagtttgatt tgactgaaaa
     2041 atatataaaa gatgatatga aaaaagagct agtagcatca aaatatttaa atgaaaaatc
     2101 aaaagttact gataaagaag cagagaacta ttacaataaa aacaagagca attatttaca
     2161 agttagagct tcacatattt taataaaaac tgttgatgat aaaggtaagc aagttagtag
     2221 tagcaaaaaa gctgaactta agaaagaagc tgaagaaata cttaaaaaag cccaagctgg
     2281 ggaagatttt gctactctag ctaaaaaata ctctgaagat agtagtgcag aatcaggtgg
     2341 agatttaggt ttctttggta aaggtcagat ggtggaatct tttgaaaaag cagcctttgc
     2401 attgaagaag ggagaggtat ctaacaaact tgtagaaagt gattatggat atcatataat
     2461 taagaaaact gatgaaaaat atcaaccatt tgaagaggta aaatcagatt tagtaagcag
     2521 tttaacatca gaaaaacaaa acctacttat acaaaatcta aaagaaaaat ataatgttaa
     2581 aattacagat aaatatcaaa tgaaagctaa agaatagata aagtcaattt attaaaaaaa
     2641 agaaaaatta actgtatgga taaaagctat ttttactctt aatatagaga gtataaaata
     2701 gcttttatt
//
LOCUS       DAEMHW010000316         2652 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.318, whole genome shotgun sequence.
ACCESSION   DAEMHW010000316 DAEMHW010000000
VERSION     DAEMHW010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.318"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(922..1959)
                     /locus_tag="KPK67_003705"
     CDS             complement(922..1959)
                     /locus_tag="KPK67_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG2460617.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(2056..>2652)
                     /locus_tag="KPK67_003706"
     CDS             complement(2056..>2652)
                     /locus_tag="KPK67_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG2460618.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKEDLTVEEILEKFEYAV"
BASE COUNT          879 a          404 c          340 g         1029 t
ORIGIN      
        1 tagctaccat cagcagacat atctggaaaa tctaagattt tgtaagttat ataaaccccc
       61 agtgccataa tcgataaaat taaactttgc gtcattacag atataatacc tgacataaat
      121 aaaacactcc tttgaaaata tttttataat tttaattatt tgctaatttt gtgtataaaa
      181 aaaccacata gtgtacaaaa ctatgtggta aaaacacgaa taagccacat agtttaatct
      241 atgtggctct tatatagtta aatgaaaact ttataaacaa tatagccaac atagatacaa
      301 tcccaaaatt ttagtagtct taaggggtct gtatctatgt taaaacgtag gtacaaaccc
      361 taaaaatagt ggctataata ataatatttt ctattgttta ataaagtatt cataattatc
      421 tctccctttt aatattttaa ttagtcagaa aattatatgt attctcgact tgttataaat
      481 tataaattat acaataataa aagtcaactg ctaaatttaa ttaaaatact aaataatgta
      541 tactcagaat acttttaaat taatgtatta tttgcaaaaa acgttatcct aagttataag
      601 tgaatgtgga aattttatca aaatatttac tagatatgga ataactagac atgtatatca
      661 atcaaatatc ataaaaaaaa gctgtctgga gaatattaaa attcttttgg acagctcaag
      721 tagtgattag aaagttatat aactttcata atcagtcgtc aatatagacg acttttatgg
      781 aaaatgaata actttatatg caatattcaa attgctacaa taagcacaat tttcaaaggg
      841 ttagggtgga taagagtact tattatataa agttttcata ttacctactt catattacct
      901 attttttgaa attaataagt tttattttgc agattttgca ttttcatctg actttatata
      961 ctttatatta tctgctgata acttttgcat gtcaagtgct tttagtgtat cttcgttaat
     1021 tattatttca gtttcatcta atgtagtaac tggtatatcg ccaacagatt ttccttctaa
     1081 tacttcaata gcaagttctc cagccttata acctagtttt tcataatcta ttccacaaca
     1141 agctaaagca ccagaagaca cagaaccttc ttcagaggct attataggaa tcttgttttc
     1201 attagcaact ttagaaacta ttggcataga agaaactatt aaattgtcag tagggacata
     1261 taaaacatct attttgccaa ctaaactaga aatagcttgg ttaacttcac ttgaagagct
     1321 gattcctttt tcaactactt cataattatt tttcttagca taatcatgta gagaatcaac
     1381 ttggattttt gaattaactt cactagtatt gtatataacc cctattttct ttgcttttgg
     1441 agtcaatgtt ttaaccaatt ctaatgtttt atcaattgaa agataatcag atgtaccaga
     1501 aacatttcca cctggttttt caagagattt aacaagtcca gcttctacag ggtctgtaac
     1561 agcagtcatt attatcggaa tatctttagt agcattataa gctgcttgtg cagatggagt
     1621 agatatagca tatatcaaat cttttttgtc ggatacaaac ttactagcaa tactttgtgt
     1681 agtaggcata tcattttgtg cattttggaa atctattttt atattatctc catctttata
     1741 gcctttatct tcgagtgctt tgatgaatcc tttctttgct ttatccagag atggatgttc
     1801 cactaattga gtaataccta tatttttttg ttttttgcta tctgtttctt tattttcatt
     1861 tgaagcattg gagccatcat tttgagaaca tccagtaagc attgatacac caagtactcc
     1921 tgctataatt aaacttaata tttttttact ttttatcatt ttattttcct cccttaaaat
     1981 ttattaatta tatttgttca tatattcata tgatagatta taaatatttc tgattaaaac
     2041 taagatttta atttcttata ctgcatattc aaatttttct aagatttctt caactgttaa
     2101 atcttctttt tctttatctt ttacatctaa tacaacttca cccttatgaa gcattatcaa
     2161 cctatctcca tattgaagag cgtgtttcaa gttgtgagta accataagtg tagttatatt
     2221 tttctctctt acgattttat ctgttaactc tattacttca tttgatgttt ttgggtcaag
     2281 tgctgctgta tgttcgtcta gtaataagac cttaggactt gctaagctag acataattag
     2341 tgatagtgat tgtctttgac ctcctgaaag atattgtact ttaatatcta aatatttttt
     2401 taaatccaaa gaaattcctt caagtaagtg ttccaagtca ttcgttttat gacgaagaca
     2461 tttttttaag ttcaataatt tccctttatt aagtgctagg gataaatttt ctctaaccgt
     2521 catagaagga caagttccaa ggcttgggtc ttgaaaaact ctacttacta tttgagttct
     2581 tttatactca gctaaatttg ttatagtagt actatctaaa gtaatttgac cagaagattc
     2641 ttttataagc cc
//
LOCUS       DAEMHW010000317         2625 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.319, whole genome shotgun sequence.
ACCESSION   DAEMHW010000317 DAEMHW010000000
VERSION     DAEMHW010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.319"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(58..1425)
                     /locus_tag="KPK67_003707"
     CDS             complement(58..1425)
                     /locus_tag="KPK67_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG2460619.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(1675..2139)
                     /locus_tag="KPK67_003708"
     CDS             complement(1675..2139)
                     /locus_tag="KPK67_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBG2460620.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(2144..>2625)
                     /locus_tag="KPK67_003709"
     CDS             complement(2144..>2625)
                     /locus_tag="KPK67_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBG2460621.1"
                     /translation="IRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKV
                     IYKNKEEDNVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNKFIGENCKGI
                     EKVKRIKAVLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
BASE COUNT          925 a          470 c          286 g          944 t
ORIGIN      
        1 acaaattcta atttttatct aataaaatat tttcataaaa aatttatatt gtatatctta
       61 ttcttttatt gctttaggtt ttgatagaaa acatttataa aatataaaga aagaaattgc
      121 tgttatagta gcagctgtta tatctgcaac tggctctgct aaaattactg caaaaagttt
      181 ttcattaaag aatgttggga gtataaatat taaaggtatt aaaagtacaa tctttctaag
      241 tagagctaaa accaaagata ttgttgcttt tccaagtgct aaaaatgtct gttgacaagc
      301 tatttgaatc ccaaacataa aagcaccagc aaaatatatt tttatactcc aactagtcat
      361 actaagtaac tgaggattct tattaaatat actaacaaaa aatgcaggaa atatcattaa
      421 taaaagccat aaaatagtag aatatgtaaa acatgacaat aaacacaact taaatgtctt
      481 ttttactcta tcaagttgac ctgaaccata attataactt attattggtt gtgctccttg
      541 tgtaaccccc atattaggaa gcataagaat ttgcattata ctgctcatta tggccattga
      601 acctactgct aaatctcctc catacatttg taatttacta tttaaggcta taagtactaa
      661 actttctgta gattgcatta taaatggaga gattcccaag ccaagcactg gtaagatgat
      721 acttgctttt ggtatcatat acttcttttt tattttaagt atacttttct ttccaaagag
      781 aaacataagt acccaaatag cagaaacgcc ttgtgcaatt atagtagcta atgcagctcc
      841 ttttacaccc atattaaagc caaatataaa aatcgggtca agtattatgt ttattaaagc
      901 acctatagca actgtcatca ttccagttgt agcaaaccct tgggtattta taaaggtatt
      961 catacccatt gaaatctgaa caaacactgt acctattaaa taaatattta aatagtccat
     1021 tgaatatcca attgtagatt cacttgctcc aaacatccaa agtaaaggct cttttccaat
     1081 cttaaaacct atagtcaaaa gtattgctaa aatcaccagt acagaaaaac tgttactcat
     1141 tattttctct gcctcatcat tgttttgctc acccattttt atagcagcta atggacctcc
     1201 tcccataccg attaaataac tacatgcagt tattataatt attattggaa atgccactcc
     1261 aacaccagcc atagcaactt ctccattttc cattctacca ataaaaatcc tatcgactat
     1321 gttgtataaa acatttacaa tttgtgcgat aatagcaggt gtagcaagtt taaataataa
     1381 tttacccaca ctttcactac ctaagttagt gtttgaattt tccattttaa aacctcctca
     1441 atccaataat taaaatgtat cactaaaatt ttattctata taaaaatttc attttattag
     1501 cagacaaatt tagagtactt tccctacaag taatactata ctatatttct tttgccatag
     1561 taaacatttt agaactaact gaatttaaat actttatctc attttttatt tattattaaa
     1621 aatttttcac atatactcat aaaaaaacta tccaagtata ttacttagat agttttatat
     1681 tatatgtaaa gaaagagctg aaactataac ccctgataac aaattgccaa gaaatactcc
     1741 cataaaagca tcttttattc tcatttttaa tatggaagct atagcagatg cagtccatgt
     1801 tcctgtagtt ggcagtggta tacctacaaa tagtattata ccaattatcg aaacactttt
     1861 taatttcttc attcttgagt ttatttttct atcaattaca tcagcaatcc ctttaaatag
     1921 tttcttccct cttaaaaatt gtaatacatg tctgaatgtc aatatcaagg gaatcgatac
     1981 caatgtagaa cctattacac taaatacata aacccatata ggatttaagc ccattgcaat
     2041 acctatagga atggctcccc tcaattctgt tacaggaacc atagataaaa ttaatatgtg
     2101 taaatatccc aaactattcc ctcctattct aagtgacatt aagttatgaa tttatttgct
     2161 ctataacctc accatccttt ttactaacta aaaatgcatt tttagtcaat gaaagcattg
     2221 gtaaatcact cttagagtct gaatatgcat atgaattttc ataatcaatc tctatcccaa
     2281 gtttacctaa tacagccttt attcttttta ctttttcaat tcccttacag ttttctccaa
     2341 tgaatttatt tttatatctt gaattttcat aaaaaaactc cgtgcctata acctcgtctg
     2401 caagaccata atacttaaaa tatttcatat atgcatatgg agatgcagtt atcataatta
     2461 taacattatc ttcttcttta tttttgtaaa taaccttttt catagattcg ttaatttttt
     2521 tcattatgca atcatcaaaa aactttttta aatcttcttc actaaaatct actattggat
     2581 aaaagattgc ctcttttgcc ttcttcaagg gtatcattct aatta
//
LOCUS       DAEMHW010000318         2552 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.320, whole genome shotgun sequence.
ACCESSION   DAEMHW010000318 DAEMHW010000000
VERSION     DAEMHW010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.320"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            6..1817
                     /locus_tag="KPK67_003710"
     CDS             6..1817
                     /locus_tag="KPK67_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG2460622.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHIGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGDGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGVYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(1905..2486)
                     /locus_tag="KPK67_003711"
     CDS             complement(1905..2486)
                     /locus_tag="KPK67_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460623.1"
                     /translation="MLYLLKINQFEKSYDIGIFKNEQSIYKFIEKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT          938 a          280 c          355 g          979 t
ORIGIN      
        1 caataatgaa gaaaaaatat gattttttaa tttattctgc tatttggcta gttattatta
       61 ttctttttat attttctcta tatcatagta tagagcatat taaaattata agtcatattg
      121 gtactattcg cagtgaaact caaaaggttg ttaagcaaga attgaataat gaaagaaatg
      181 atgacttgat aaaacgactg gataatattc tgataaagct aagaacaggt gatggagaaa
      241 atggatttca aagatgtgat aataaagaat ttcaacaaaa actaaatcaa atggattcta
      301 tgtgggaatc tatgaagaaa gaaattataa aagttagaaa tggtgcttca ggtgataaat
      361 tatataaatt gagtgaagag tattctgtgt tgtctaatca aattgttttt atatctgaaa
      421 aacactctaa tgcaaaattg tattcttttg ctactgcact atttatttat cttatattct
      481 caacaataag tcttttaatt tgggaatatt ataataaaaa aaggtttaaa agaatatttt
      541 atactgataa tttaacaaaa ataaaaaatc aggttgcatt tgaaaacaga gcgattgaaa
      601 ttttgcacaa tgcatctaac aaagagtatg tactgctaaa tattgatatt gacaatttta
      661 aatatattaa tgatacacat ggatatgaat atggagataa agttctaata atagttgcag
      721 ctgcactttc taaaactttt aatatcaaag aaacttgtgc aagaattggt tcagataatt
      781 ttgtaatact tgcaaaatat agagattctc ttttagagga tattcgggaa atgctaacta
      841 atgctataat ctctgaatta gatatgaatg taactcaaac tatatcttat tgtataggag
      901 tgtacttagt tgaaattgat aatttaggtt ataaatctat caattctata atggataagg
      961 caaatattgc acacaaggta agtaaaacaa gaggaatttc ctccacagta tggtataatg
     1021 aaaatctgtt aaaacagtta caaatggaaa atagtattta taattatatg tataaagcga
     1081 tagaaaatga agaatttcat atgtatttgc aacctaaatt tcaaatatct tctttaaatg
     1141 ttgtaagcgc agaagctctt gtaagatggt tttctccaga acttggtttt ttatcaccag
     1201 atgaatttat tccattgttt gaaaaaagtg gatttattat tgaattggac ttttatatgt
     1261 taaaaaaagc gtgttcattt gtaaaaaaaa cgtttatgaa aaaaaatcaa tatacatatc
     1321 cgattgcagt taatttttca agggttacta tttaccaaaa tagtttttat caaaggtttt
     1381 tagatactgt taaagaatat gaaattcctt ttaagtatat agagatagag gtaactgaaa
     1441 gtgcttttaa tgaaatatct caaccagtta tttcgatttt agaggagctt aaaaagttag
     1501 gatttttaat ttcgatggat gattttggat caggatactc ttcattaagt cttttatgtt
     1561 ctctatctat taatggattg aagttagata aaagcttact taaagaaaca tttaaccggg
     1621 agaaagtata tagtattatt cagtgtatta ttgaaatgtc acatcgtata ggcatgtctg
     1681 ttgtatgtga aggtattgaa actaaaaaag acttggaatt tttaaataca gttaaatgtg
     1741 atgtaggaca agggttttat ttttctaaac cgatagaaga aaaagaattt tttaacaaat
     1801 atgtaactaa aaaataatat aattattttt atcagtctat acattgagtc attttatatc
     1861 tcgttaaatg actcaatgta ctgttatgta ttttaaaata taaattattt aaaacgtttt
     1921 ttgtacttat atagaaattc ttctatattt tttgattgtt ctcttatttc aactgcttct
     1981 atatctagta aataaagtat agaatcattt gtaagttcaa catattcacc atcttctgaa
     2041 ccgagtgcat gtctctcaat ttttaatcct ttttcttcat aatatttttt tgtttctata
     2101 tatagtgatt ctcgcttttg tatgtagtct ttcacttcat tatttggaaa gttatatgaa
     2161 tccactaatg tttcgccatt tataaaagtt ttctcggtag caactttttc atcccaacaa
     2221 tggatttctt cccaagtaaa aaatatctca gttccatctg gaatgaatga aaacctggta
     2281 ataatatata aataattatt gaaatttact tcataatatt caggaatatc ttcaaattcc
     2341 atatagtaat ttgtataatt aaaattacta ttgtcttttt ttacaaaggg tattttttca
     2401 ataaatttat atattgattg ttcattttta aaaattccta tatcataact tttttcaaat
     2461 tgattaattt ttaataaata caacattctt tctcctttta ttcaaaatat aatttttata
     2521 aatttactaa tctttaggtt ttttaattat at
//
LOCUS       DAEMHW010000319         2505 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEMHW010000319 DAEMHW010000000
VERSION     DAEMHW010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.321"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(160..360)
                     /locus_tag="KPK67_003712"
     CDS             complement(160..360)
                     /locus_tag="KPK67_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG2460624.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(585..761)
                     /locus_tag="KPK67_003713"
     CDS             complement(585..761)
                     /locus_tag="KPK67_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460625.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(815..904)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1084..1284)
                     /locus_tag="KPK67_003714"
     CDS             complement(1084..1284)
                     /locus_tag="KPK67_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2460626.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1286..1636)
                     /locus_tag="KPK67_003715"
     CDS             complement(1286..1636)
                     /locus_tag="KPK67_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBG2460627.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1798..1986
                     /locus_tag="KPK67_003716"
                     /pseudo
     CDS             1798..1986
                     /locus_tag="KPK67_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          995 a          326 c          275 g          909 t
ORIGIN      
        1 tactttataa atatattttt gtcagattta ttttataaaa agtatttaga ctaagtatta
       61 tttttaagta tttttttgtt gaagttattt ttttcttttt tacaaaatgc gtaaataaaa
      121 aaaagactct taaaagagtc tttccgaaaa atctatatat tataatatag ttatattttc
      181 tgcttgagga cctctagcgc ctttaactat atcaaagctt actttttgac cttcttctaa
      241 tgatttaaat cctgaagttt gtatagctga gaaatgagca aatacgtcat ctccactttc
      301 cattgatata aatccaaatc ctttttcatt attaaaccat tttactattc cattattcat
      361 taaaaatact cctattaacc tatatcaaga tatacttgat ttacttgtgt attaccacaa
      421 atactattgt atcatagatt aacataaatg tctatttaat tataattttt agttaacatt
      481 tttatttatt cttaatatga aaataaattt aaacatttta tattaccaat atattaatgt
      541 aaatgtagat aaaactaaaa agggttttct agaaaaaaat ctgtctaatt atacttactt
      601 accaatacca tcacttgatg caagcaagat tccacaactt tttctccgta ctctaatata
      661 aaatcatcta actgtgtatc tataaaatca caataatcat atgaactacc aatataagca
      721 gttaacatat gtgtacattt atccatttta tctctaaaca taatgtattt atccccctaa
      781 aaaactttca tgaaaaacaa aaagaaaata tctttttggc aactggctga cataacttat
      841 tttataagcg ttagtcccta tagctttgcg tcactaaatt tctctagttt tgccgattta
      901 gttttattct acagattaat aatacaatag aattagttat tattcaacat atctagctga
      961 aaaattaata aatttatata tgaacgttaa attatctgaa aaaataaaaa gcccctctgt
     1021 caggagtgac ttaaaaattg aatttaagtt tgtatttaaa acacattttt aataatgcaa
     1081 aaactaatca aaaataaaca attcatcaat aggtgtatct actatttttg atatatctat
     1141 tgctaatttc aaagaaggat tatactttcc tgcttctaaa cgcataatgg tttctcttct
     1201 aactcctact ttttctgcta attgttcttg agttaattct tttaattgtc tatatttttt
     1261 taggttacaa atgaatttgg gcatattatt tttcctctaa aatataaagt tttatagcat
     1321 aaacatttaa agtgattgca tatgtaattg atagtaaaac acacattaat tctatattcc
     1381 tagacactat agtaatacac cataaaatga ataattctat aatagcaagc tgtcctgtaa
     1441 atgccatagc tgctttttca tcttgtacat acctttcatc tgctttatca ccatttattt
     1501 ttgctataat aaagtttgag aaaaaagcaa aaaatccaat atatgtaagg tcagcaacat
     1561 tgccagatga aaaatatcta agacttaaaa atccaaggaa accacaaaaa cccataaaaa
     1621 attttttctg aatcattttt tatcctcctt agtaatttta aaagagatat atttgtatct
     1681 ttatgatata aatatatctc ttttatattc ttttgtcaat agaaaatcag tattatacaa
     1741 tatttgtact gcaccataat tgttagagca aaaatctaat gcttgagatg tttatgtatg
     1801 actaaatatg attttaattt caaattaaaa gtagctaaat tttatttgag tagagaagga
     1861 agagatattt taatagtaaa acaatatgga gtttataatc attctcaagt ttaaagatgg
     1921 gttaatatat taggtgagtg tgcactaaaa ataaaaaaat tttctatact atatggttca
     1981 agttagatgt tataaactat atgatgataa ctaaatatag tgtacaataa atcgctaagt
     2041 attttggaat aaacaatagc acagttatag caacatgaaa ataaaaattt ttacatagtg
     2101 gtgttgatgc cttttccaaa atgaaaggaa gaccataatg aataaaaaca aaaacactaa
     2161 agaaaaaata aattaacaaa agaacaagaa ttagaatatg aaaataaatt tttaaaagat
     2221 gagcttgagt ttttaaaaaa gatgtgtgcc tcagggaagc cacattaaaa aacagtggta
     2281 tcattattaa tcaaaagaaa gttagatgta ttatcaaaaa attagaactt aaatgcatag
     2341 cattttttca taaatcacat aaatataact cctataaagg tataattgga aaagtaaaaa
     2401 ataataaaac aaatagaaga tttaaaatat caatagcata tcagaaaata acaaatgata
     2461 tgctagaatt tataaaatat atctaatgat aaaaatggaa aatca
//
LOCUS       DAEMHW010000320         2485 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.322, whole genome shotgun sequence.
ACCESSION   DAEMHW010000320 DAEMHW010000000
VERSION     DAEMHW010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.322"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            986..1699
                     /locus_tag="KPK67_003717"
     CDS             986..1699
                     /locus_tag="KPK67_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2460628.1"
                     /translation="MIKVAICEDEKVHQELLIEYLQKLFNELSIDYDLCIFNSGEQLL
                     ENYPEDMDIFLLDIQMGELSGMDIARKIRDIDSNTVEIIFTTSLIEYIQEGYEVRAYR
                     YLLKPIEFNDIKNHITACIEEIHNRRDKYLVISNKYENYKIKIENITYIEVQNKDITI
                     HTLDNDYEIKMSMNKIEKELKKYNFIRCHKSFLININFVENVKQYVAILEDGQEIPIS
                     RYKFKEFKTIFLKSLRKVL"
     gene            1918..>2485
                     /locus_tag="KPK67_003718"
     CDS             1918..>2485
                     /locus_tag="KPK67_003718"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460629.1"
                     /translation="MFLYKLTFEISYKDSFLYAFSFAFLYSIIGRIIYTFLINFLFDK
                     VVEYSALGYHSLISIILNVILILLLKYFKQIINEELDIKYYIYVGLTVIINGISILFL
                     FISGEYINDLYKIMEYSDAIPRMSVIPYIQTIQSIIPAIMVFCNIFLIIIINNLIKGM
                     KAKSELESINNKLNMQYNYYLNIQESHMK"
BASE COUNT         1056 a          187 c          325 g          917 t
ORIGIN      
        1 tagatagtaa aattgatagt gatattttga ggatataatt atattattta tataatattg
       61 atttggatga tttatttata ttttgtaaaa tagttattaa gaatcttttt atcgtaagat
      121 cttttaataa ttgttttaat aaggttttgg agagacaaga tacctgagaa aaaagtttaa
      181 attaaataat gttaacataa tataaaatat aaatatgtca tatgaagtat aaatatatgt
      241 gacataaaac atgaatagta tataatataa gtaaattatt agatatcaag tgtagaaaaa
      301 aacattttta ttgcaaagaa tcattgacaa attaaatttt tgactttata ctcgatacaa
      361 ataagagacc ttttaaaaaa tactagaaat aaattccaca acataaaata tacagctaca
      421 aaataaataa aattataaaa atcgctatga atttatgtaa aatgagtttt taaaaaagta
      481 ataaatcaat taaaaagttt taaattaatt tgatttaata tgcgaatatt taataatata
      541 gtattacaat taataaaata ttcttatgaa tcttataagg gatttttttg aattgatagg
      601 taattcaaaa tctatttata agcgtaagta tgtagaatta ttctacaatg ataatataaa
      661 gttgaaagtc aaataagata aaccaattga ttttaaaaag taggtcaatt taaaactaat
      721 gaatgattgt ttgttatagc taaattgtta tgtttaaata tagtaaaaat ataaatagtt
      781 tttaaatata atcttataag caactattta atttgaaagt gtatatttct taaagttaaa
      841 ggtattggat atactatgat aaatagatta gctttataac gatgatttaa tgtttaaatg
      901 taattaagtg aaataaaaac taataaattc tttgctttaa atatatggta aggtataatt
      961 ttaagtttaa atataggaga atattatgat taaagtagca atttgtgaag atgaaaaagt
     1021 acatcaagaa ttattaatag aatatttgca aaagttattt aatgaattgt caattgatta
     1081 tgacctctgt atattcaatt caggagaaca attattagaa aactatccag aagatatgga
     1141 tatattcttg cttgatatac aaatgggtga attaagtggt atggatatag caagaaagat
     1201 aagagacatt gatagtaata ctgtagagat aatatttaca acttcactta tagaatatat
     1261 acaagaaggg tatgaagtaa gagcatatag atacttacta aagcctatcg aatttaatga
     1321 tattaaaaat catatcactg cttgtataga ggagatacac aacagaagag ataagtactt
     1381 ggttattagt aacaaatatg agaattataa aattaaaata gaaaatataa cttatataga
     1441 agttcaaaat aaagatatta ctatacatac attggacaat gattatgaaa taaaaatgag
     1501 catgaataaa atagagaaag agctcaaaaa atataatttc attagatgtc acaagagttt
     1561 tttgattaac ataaattttg tagaaaacgt aaaacagtat gtagctatac ttgaagatgg
     1621 tcaagaaata ccaataagtc ggtataagtt taaggagttt aagacaatat ttttgaagtc
     1681 tttaaggaag gtactataat gaatgaagca ctaattttaa atataattga tataataaat
     1741 tctatactgt atttagtaat tttttattta atgcttacta aaattaaagc tagaaaaaca
     1801 agtataaaaa aaataaatta tattataatc tttgtcctta ctatattaat attaatctca
     1861 cctaaattta aggatattgt aataataaaa tataatatcc ttgaggggtt tttattaatg
     1921 tttctataca agcttacatt tgaaatttct tacaaagatt cttttttata tgcttttagt
     1981 tttgcttttt tatatagcat aatagggcgt ataatttata catttttaat aaatttttta
     2041 tttgataaag tagttgagta ttctgctttg ggctatcatt ccttaatttc aataatatta
     2101 aatgtgattt taatattatt gcttaagtat tttaaacaga ttattaatga agaattagat
     2161 attaaatatt acatatatgt tgggttaact gtaattataa atggtataag tatattattt
     2221 ttatttatat caggagagta cataaatgat ttatataaaa ttatggaata ctctgatgct
     2281 attccaagaa tgagtgtaat tccttatatt caaactattc aaagtataat accagctatc
     2341 atggtatttt gtaacatatt tttaataatt attataaata atttgataaa aggaatgaaa
     2401 gctaagtcag aattagagtc aataaataac aaattaaata tgcaatataa ttattattta
     2461 aatattcaag aatctcatat gaaag
//
LOCUS       DAEMHW010000321         2439 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.323, whole genome shotgun sequence.
ACCESSION   DAEMHW010000321 DAEMHW010000000
VERSION     DAEMHW010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.323"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(550..1062)
                     /locus_tag="KPK67_003719"
     CDS             complement(550..1062)
                     /locus_tag="KPK67_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460630.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            1615..>2439
                     /locus_tag="KPK67_003720"
     CDS             1615..>2439
                     /locus_tag="KPK67_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBG2460631.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAKQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSE"
BASE COUNT          888 a          341 c          211 g          999 t
ORIGIN      
        1 cctatttcaa atctttttcc ttcagtataa tctttttctt ttaatttatt ttatattttt
       61 gacataatta ttcttacatt taattgtttt ttatttttaa cacaatcgtt ttttgttaaa
      121 tcattttata ttcttgatac aatcattctt acattctatt attgtttatt tttgacgcaa
      181 tcgttttttg ttgaatcatt ttatattctt gatacaatca ttcttacatt ctattattgt
      241 ttatttttga cgcaatcgtt ttttattcag tcatttaata ttttcgacac aatcattctt
      301 acattctatt attgtttgtt ttgatacaac tgtttttatt cagtcatttt atattttcga
      361 cacaatcatt cttacattct attattattt atttttaacg caatcgtttt ttatttagtc
      421 attttatatt ttcgacacaa tcattcttac attctattat tgtttgtttt tgatacaact
      481 gtttttattc agtcatttaa tattctcaac acaattacct tttcattcaa tcaaagtaat
      541 ctttaagcat tatttttcaa gctcatcaaa gtaattatat acgataacct cacctgtctc
      601 tttatctgta gtctttataa tgcccttagc tccatttacc tcttcttttg taaattcatc
      661 atctaacaca actttgaatt tatcttcctt tccataacct tgttgaaatt catctctcaa
      721 aattgcattt ttttctgctt ttggcttcaa ctcaaattca attccagatt tcatttcatc
      781 aaaataattt aatactacaa cctcattatt cattttatta gttgtcttta taatcccttt
      841 agctccattt acttcttctt ttgtaaattc atcatccaat gttatattaa atttgtcttc
      901 ttttccatat tcctcttgaa aatccctctt catttcattg tatttagttt caaactcttt
      961 actcttgttt aattccatag ctgatacatt tgtcaatgat gctccaattg ccattactgt
     1021 taatgccatt actataccac ttttaaattt ttttactttc atatcttacc tcctaaatta
     1081 aatgttctca atgaaattat ataaagaaaa tcctaagttt tacagtttca ttcttgtaac
     1141 taaaattact attatgtaac caaacttttt gcttcaacta ttccttaaaa gttgcttttt
     1201 tgaggaatag ttgaagttaa ataattagat tttatctata tttatagatt taaaatcaaa
     1261 tttatccata taaataagta cttatttttg caaaataaaa atttttaatt actccaatcc
     1321 ttcttattta cttaaaattc tatgataaga aatttaaaag tctttacctt ctttaacaat
     1381 aatttattta cttttaacat acaaaatata tagagtatat caaaatcatt ttactgaata
     1441 ttctaaaaat aagtaccccc tattagtata tatatttaca tattaaaaaa aagttttaaa
     1501 taaagttata ttttttacaa cagttttgta attaaaaaca tattgaaggt aaatttaaag
     1561 gtactatata ctataaatat gtcgacacaa taaatttaaa aaggagaact aaatatgaaa
     1621 aaaagattaa taataatgat attgtctgtt gtcttagtac tatcaagtat acttacgata
     1681 tttgcatatt caaatattaa atataataat aataaattga tatattctaa tatgatagat
     1741 aaaaaaactc aaaatagtgt aaaggaaatc ctaaaagaaa ataaaattaa tgaaaaagac
     1801 atagatacat ttattaaagc agtcaacaat tataataaat tgcaagttaa aatacttcaa
     1861 aataatataa atatatctaa atctggatat tcttcaatca atgcaaaaca agttccatat
     1921 aacctagaaa aattgcaaga taattgggtg aaaaaattcc ctgattatat ggatgtaaat
     1981 tgtagaataa ctgcttttag actttttaaa gattttataa attcaaataa gaaatttact
     2041 ggagactcta ttgatttgaa tgtcgacttg gacactataa tgaataataa agatgctaaa
     2101 tttagcacca aagatgtaga aaaatttatc aacttctttt ctgcaatacc agctaaagat
     2161 acagatgata caatcaaaat tgcaaaacaa ataaaaaatg aatggaaaaa aagaaaaata
     2221 tcttttaaaa ataataaaaa tatatctatt ataaatggat ttcttcgtta tccagaaact
     2281 aaaaacgtat ttataggtca tacaggcata tgtattaaaa ctaaaaatgg cattctattt
     2341 ttagaaaaat atggtgttac atcaccttat caagttacta aatttaaaaa taaagaagat
     2401 gtaaaaaatt atatgtttaa tagattgaag atgtcagaa
//
LOCUS       DAEMHW010000322         2416 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.324, whole genome shotgun sequence.
ACCESSION   DAEMHW010000322 DAEMHW010000000
VERSION     DAEMHW010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.324"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(176..2269)
                     /locus_tag="KPK67_003721"
     CDS             complement(176..2269)
                     /locus_tag="KPK67_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG2460632.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYVCNMKTGVFRYSPSQVELFDLPGEIVENPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT          809 a          399 c          238 g          970 t
ORIGIN      
        1 tttttattat ggttatccct ttttaaaata ctttaatcat atactattcc caatttaaca
       61 tccattgaaa tgctattttc ttatattgca atttataatt gatatatctt caatataata
      121 atttaatgta tttttgattt taaagctttt cagtgatatt attgataaat tagaattaat
      181 tatctagctt taaaaggtca aagatttctg ttgctgtctg cggttttcca aataaatatc
      241 cttgtatgta atctggcttt atttgtttaa ctaaatcata ctcttcttgt gtttctacac
      301 cttctaaaca tacttttatt cctacgtcat gacaaattgc aacaataaaa tgtataaatg
      361 tagcatcaaa cttactcttt aatatatctt taacaaatac tctatcaatt ttaactatat
      421 ctattggaga aaattttaaa acttctagag acgagtatcc agtaccaaaa tcatccatag
      481 caatataaat tcctaaatct tgtaaagcct taaatttaaa ctgtaaaata ctcatatttt
      541 gtactgtatg actttctgtc aattctaaaa caagattttg ataaggaaaa ttttcatcat
      601 ctataatttt tactatatct tcaataaatg tgtcttctaa tatttgaaca gctgaaacgt
      661 ttacactcac tttaaaaaaa ggatagtaat caatccattt cctaaaagta cgtaatgcca
      721 ttcgaagcac ccaagcccct acaatattta tcatatcatt ttcttctaat attggaataa
      781 attccaaagg agaaattgct ttgcatttgt cattagtcca ccttaaaaga acttctacac
      841 ctattatttt atgactttct gtatctactt gtggctggaa tcgaagacta aaccccctaa
      901 aatcatcatt aattgatgcc tttaaatccc gcatcatttc taatgaatac attttatttt
      961 ttaatatttc ttgggagaaa aaaacaagcc tgtttttacc atgctctttc gcatattgta
     1021 aagagtaact agcgcattta taaagttctt ttactgtatc tccatgcttt ggatatatta
     1081 cacaaccagc agaaattgtt atatttaatc catattttct ccatagttga aggtgtataa
     1141 tcatattctg tatttgatta tatagagtta gaatttcatt ttcttcaaca ttatcaacaa
     1201 gaatacccat ttcgtcccca tcaagtttat aaagttcagc attatctggt aaaatagatt
     1261 gtatagactg tgctagtgtt tttattatat tatctccaaa atccctatca tacatctcat
     1321 taacattttt aaaatcgtca atatttaata atactatgca taatttttca atcataggat
     1381 ttgaaatttt atcttcaaat acactcataa attcatgata attcagtaac tgtgtcaatg
     1441 aatcaatctt attttgttta cctaattgcg tcattattcc tgcaaaaata cttggttctc
     1501 caaattcatc acgcattagt tgacctctac atcttaacca tatatattct ccactacgat
     1561 ttttagccct aaattccact gtatgataat ccatctggtt tttacctatt tcagtgtttg
     1621 acttataaaa acgattccag tcttctggat gtacaatttt tttccagtaa acaagcggat
     1681 tttcaacaat ttctcctggt aagtcaaata attcaacttg tgatggtgaa tatcgaaata
     1741 cgcctgtttt catattgcaa acgtatatat attcatctgt acctctaatc aaagcatcat
     1801 aaagcaatgt agaattataa tcaaaattgt ctcttgaaaa attaaaaact cctttttctt
     1861 ctttattatt tgatatttca tgaaattcat taatcttaat tggtttattt aagcttgttg
     1921 agtttgcaat atctattgat tgatgcatat gaacaactgt gccatcttgc catgtaatta
     1981 aactatcata attttcaaaa acacgattta acttattaca tttttcatac cactttatta
     2041 atactccttt tttatcattc tttaataatt ctaaaacttt acaaaaacta caagtagaat
     2101 ttttttcagg atataatact tgccaacaaa cttttccttc tggatctaaa atattatatt
     2161 cttctttcat ttttttgttc ataaaaataa tttcattagt atgaatattt gtaacatata
     2221 tattaatctg tagttgattt aatataactt caaaattatg tttgttcata tacttatctc
     2281 ctccatagtt actgctcttt ataacaacat tgttaaaatt attcttttta gaagatttac
     2341 ttataactta tacatataca ctaaatacct taatttaatt agaaagttta ttacttcata
     2401 ttttaaatta taattg
//
LOCUS       DAEMHW010000323         2361 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.325, whole genome shotgun sequence.
ACCESSION   DAEMHW010000323 DAEMHW010000000
VERSION     DAEMHW010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.325"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(100..708)
                     /locus_tag="KPK67_003722"
     CDS             complement(100..708)
                     /locus_tag="KPK67_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBG2460633.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(817..>2361)
                     /locus_tag="KPK67_003723"
     CDS             complement(817..>2361)
                     /locus_tag="KPK67_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG2460634.1"
                     /translation="SPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYF
                     ETFNIDFFDNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDY
                     WSDNKLFKLLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNR
                     AFRELFGISSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISR
                     TLYFGQKRSRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNI
                     YSSVQLIENLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIK
                     AVNFDIIDITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAI
                     KFTLEGGEILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSG
                     IGLCLTKSLIEIQNGKIYIDSKVGEGTSVKIIFPIKEVVEEVYDNSNYNDNIEKFEIE
                     FFDIYK"
BASE COUNT          751 a          403 c          188 g         1019 t
ORIGIN      
        1 tataaagtta tttttatcaa actaaaaatt ctataaaaaa ataagataca tacctataaa
       61 ataccttcct atagatacat atcttaccta tataaaaact tattctatat aaaaacgcca
      121 taaaaaatct tttgcttctt ctgcataatc tatatttata cgtttactag tttttatatc
      181 taactcatct ttactaaata ctttttttcc tttatcatca taataaaaat cacttatata
      241 taattcttca cccattatac tcttactatt tagacttcta tctattttta aagctttgca
      301 aagttttcct ggtccgttag taatattttt tacttgatat ttactcaact cagtgtaagc
      361 tttgttgtat ctattcatag atatctcatc taaagaagtt ataggctcta cccctcttat
      421 taatacacac tctggcacat tttctatatt tgcagataaa ttcaagcaat tatacatacc
      481 ataaattaaa taaacatata tatgtccacc atctaaatat aatggcttag ttctatctgt
      541 ctttttattg ccatatacat gtgctccttt atcatttaca cccatgtatg cttctgtctc
      601 tatgattttg gtaacaatta ctttattttc atatttcctt attagatatt tacctaatat
      661 tgatttggct aaatcaattc cattttttct aaaaaaatcc ttttccaaaa tttcacctca
      721 taaaatttat ttaaaacgca tcaaatacaa ctaaagtaat aaataaaatt aactcttatt
      781 aatgtgatat atataaacaa taaatttaga catttattat ttatatatat caaaaaattc
      841 tatctcaaac ttttcaatat tatcattgta atttgaatta tcatatactt cttcaacaac
      901 ctctttaatt gggaatataa tttttacact agttccttct cctaccttac tatctatgta
      961 aatcttccca ttttgaattt caatcaatga ttttgtaagg cataaaccta taccacttcc
     1021 aaattcattt gatataccac tgttttcaat ttgtttaaat cgattaaata tatcatttaa
     1081 tttttctttg tctatgccta ctccagtatc ttttataatt atctctactg tttcgtcttt
     1141 tttatatata cctacaagga tttctcctcc ttctaaagta aattttatag catttgataa
     1201 tatatttagt attattcttt ctatcgattc tgggtcaagc cctaccataa cagtttcttc
     1261 atctgtatca aaaattaaat ctattccttt agacttagca taactactaa tagatgtaac
     1321 tattctttct gttatgtcaa taatatcaaa attaactgct ttaatcttat atatgtctga
     1381 cttaacttga gaaatattta ttatattatc tattaatctc aataatctaa acatattttg
     1441 tttagttatt tttatatgtt cttttagtat atcctgaaaa tcaattattt ttcctttttt
     1501 ataaaggttt tcaataagtt gaactgaact atatatatta tttaaaggtg tcttaagttc
     1561 atgagatata ttagcataaa actccatctt catttggtca tactgtcttt gttttattaa
     1621 tgctttttta agtctttcag actctctttg cacacttaaa tctttaataa ttctcactct
     1681 acttcttttc tgtccaaagt ataatgttct tgatatcatt tttacttctt taatgtgatt
     1741 attttcattt actatatgga tactcttctc tctttctcct atatttaaat tgtttatgta
     1801 tttttttact aatatatttc tatatttttg tgttgtaact cctaatacat cactagaaat
     1861 tccgaacaac tctctaaagg ctctatttgt atatagtact cttgatgtac ttaaatcctc
     1921 aagaaaaata gcctctggta taacatccag taatttttca tatattattt tttgttcatt
     1981 tactgttcta agaatattta tctctttagt tatatctcta aatgtaagca atttaaagag
     2041 tttattatct gaccaatagt ctccaactct aacttcttca acatgttctt caccttctga
     2101 gtcagacatc acaaaattaa ctatacagtt tgctgtgtca ttgtaactta ctatttcagc
     2161 tttcttttct tgcgttagat ttaatctttt aaattcccta ttgtcaaaga aatctatgtt
     2221 aaaagtttca aaatattgtt ctaatgtcat atcgactaaa tcacttgaaa cacatttaaa
     2281 actcttttca tattttttat ttatacttat taccttattc ttctttatgc caccttcagc
     2341 acttcttaca agtataggag a
//
LOCUS       DAEMHW010000324         2346 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.326, whole genome shotgun sequence.
ACCESSION   DAEMHW010000324 DAEMHW010000000
VERSION     DAEMHW010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.326"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            53..2268
                     /locus_tag="KPK67_003724"
                     /pseudo
     CDS             53..2268
                     /locus_tag="KPK67_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT          937 a          216 c          397 g          796 t
ORIGIN      
        1 aaaaaggata tattatctat atgaagtatt gactaattag gggaggagtc gaatgacagg
       61 gagaaataaa agttttacat taattacaat tcttttttta acatcattat ttttgacaat
      121 ggggtatatc tatatagaag atacaaaaca tcttcttatg tcagaggcaa aaatacatat
      181 aaaagaagtt gcagtacaag gctctcaact agcacaaaga caaattgaaa aagatttaga
      241 tgttttaaat attttttcta actattatgc ttcaaatcca gatataccta atgaggaaaa
      301 aatgaaaaat cttttagatg aaatggaaaa tcaaaaattt tacactatgg caatagttga
      361 tataaatggt aatgctgaaa ataccaaagg tgataagttt tcagtgaaag atagagaatt
      421 tttcaaaaac tcaattaaag gtaagaagta tgtttcaagt ccatatgttg atgaagtaaa
      481 taagtctata aagaagattg ctatttcagt accattatta aataatgata aagtagttgg
      541 agttttatat tgtacttaca atatcaatac tctgatgaaa cttattaata tatcttttta
      601 tgaaaataat agtatatctt atgttgtaaa aaatgatggt acaattatcc ttcatccaca
      661 aggggatagc ctatcaaaaa atatttataa attgttagaa caggataatg atattcaaga
      721 agtaaataga ttaaaaaaag aattgcaaga aaataaaaca ggtgcaactg tattaaatat
      781 gttggaagaa agaagatatt taggatatgc cactatggat aatgggaata gtgaaaataa
      841 ttatataaaa gactggaatt taattttttc aattccagaa actgttgtat ttagtaattc
      901 aaaacaaata atcaacagag ctgtatatgc agtgttatct attgtattaa tatttgttgc
      961 aattattttt tatataattt atataaaaaa atctaatgaa aaagaaatat taagtttggc
     1021 ttatgaagat aaagttacat atataggaaa tcaaaataaa ttttatagag agtgtagtaa
     1081 gtatctttta gataaaccat ctttaaatta tattatagta tattttgata ttaataactt
     1141 taagatgatt aatgatactt ttggatatga atttggtgac aatttattaa ttacaatagc
     1201 aaaagcttta aaagaagaat taacagaagg tgaggtatat gcacgacttt caagtgatta
     1261 ttttgcaatt ttctgtgatt ataaaaatgg aagaaataaa attataagaa aacttgataa
     1321 tatacgaagt aatattgaaa gtaatttgag tatagtcttt gaaatctctc tgtgtgtggg
     1381 tatttatttt gttgaagaag gtgagatatt caaaaggctg taaataaagc aaatatggct
     1441 aggtctgttg ctaaaggtaa aaatataaat tatgctattt ataatgaaga tgttaggaat
     1501 aaacttagtg aagaatcaat gattttagac gatataaaaa tagcattggt aaaaaatcaa
     1561 tttgaagtgt attatcaacc taagttttca cttgtaactg gagaaatgat agggagtgaa
     1621 gcactaataa gatggaatca tccagaacat ggttttataa gtcccgcagt atttattcca
     1681 atagcagaga aaagtaagtt gattttaaaa attgggagat ttgtatttga aagggtttgc
     1741 aatgatttat ctgaatggaa aaagcaaggt aaaaagatag ttccagtttc agtaaattta
     1801 tctagagtcg agttgtatca accagatatt gtaaaattta tcaataaaac aatacaaatg
     1861 tataatctaa gttcagattt cattgaaata gagataacag aaactgtggc tattaatgag
     1921 ttaaatattt taaagaatgt tttaaatgaa ttgaggaagc atggattttc tatttctatg
     1981 gatgactttg gcacaggata ttcttctatt agttgtttaa gagatatgcc aattgatata
     2041 ttgaaactag ataagtcttt tcttggtggc attgaacatg atgagagaag tcgcaatata
     2101 gcaaaatcaa tagtgtctct agcaaaatca ctagatttag ttgtaattat agaaggtgtt
     2161 gagagtaaag aacaagctga gttaatgaaa caatttggat gtgatttagt ccaaggattt
     2221 tattttgcac gaccaatgcc agctaagaat tttttagatt tactttaaaa caaaatttga
     2281 tttttatata aagcactatt atgaatgctt gattttgata aatcattgct aaataattag
     2341 tgtttt
//
LOCUS       DAEMHW010000325         2327 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.327, whole genome shotgun sequence.
ACCESSION   DAEMHW010000325 DAEMHW010000000
VERSION     DAEMHW010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.327"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..304
                     /locus_tag="KPK67_003725"
     CDS             <1..304
                     /locus_tag="KPK67_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CTP--phosphocholine cytidylyltransferase"
                     /protein_id="HBG2460635.1"
                     /translation="YDMYVGDNKGYILCGVSYWSLTDGNIIAKKLEETIENGDFESLF
                     WDDVVMKNKDALNMYIQKIDFKDCFEIDNEKDLRFSEKCIVQNNKFTMKKILQYSK"
     gene            322..>2327
                     /locus_tag="KPK67_003726"
     CDS             322..>2327
                     /locus_tag="KPK67_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBG2460636.1"
                     /translation="MGILDEIMNQEHFDIKTSLDYINEYEKLLPFDSEIHTARAVTYF
                     FGEEYKLAEEHIRKALKFRPNNYDNNFYLANILIAMGEYPDAIKSAVLSVTFLKHFAK
                     IQGYDYINDKLFYYNEQVKTLIEYCIENIKDTNVLEDLKRFFGFIELSIAKFPVCFYG
                     DKWNILVGEYLNKDLNSEYNDYICFYSDTYNNMLSDFQKKLYTVINNSEISSICPIES
                     FKSMTAKEFSLDNEKYIIPIATTEYNQVIDFKIKNTNFKSQQVDPPGFFKYYRVNNGI
                     SLNSDKDVIIGKPIKLGFKEKNKKLVFTIFIDALSQKYLLETKYKSMPYTKAFFDKGI
                     IFENCYSAGEWTYPSLTSVVTGLYTTNHHFLHSSAGYKYPDDIDLFSEIMKKNEYFTA
                     NISGTYALAPYSSGFRGFDRTVFKNSLGFSDSVIINDAIKHIESFKETNQFVFMSLSD
                     VHKILDDIENYSVNYDIVNQTSLDIETSLSKRSSFVSAWQKYDEEAIKKYDVALRELD
                     RQLKGIYDYILQNYSEEEYLISIFSDHGASFLGKDDFLLKSNITNSVLMVRGSGSASN
                     GNTEYISNMDLIPIILKLANIEHDLSKYDCVLPKTLGGKGREFTYSESIYPGQTYKAM
                     VNNDKYCYILETKGITDIDGTINFDEYSDSVFDKKEDKEVKDND"
BASE COUNT          903 a          219 c          395 g          810 t
ORIGIN      
        1 ctatgatatg tatgttggtg ataataaggg gtatatattg tgtggtgtat cctattggtc
       61 attaacagat ggaaatatta tagcaaaaaa attagaggaa acaatagaaa atggagactt
      121 tgaaagtcta ttttgggatg atgttgtaat gaaaaataaa gatgctttaa atatgtacat
      181 tcagaaaata gattttaaag attgttttga aatagataat gaaaaagatt tgagattttc
      241 ggaaaaatgt atagtgcaaa ataataaatt tacaatgaag aaaattttac aatactctaa
      301 gtaggttagg aagggatttg tatgggaatt ttagatgaaa taatgaacca agagcatttt
      361 gatataaaaa catcactaga ttatattaat gagtatgaga agctactacc atttgatagt
      421 gagattcata ctgcacgtgc agttacatat ttttttggag aggaatataa acttgcagaa
      481 gaacatatta gaaaagcatt gaaatttaga ccaaataatt atgataataa tttttattta
      541 gcaaatatat tgattgctat gggtgaatat cctgatgcca ttaaatcagc tgtgttaagt
      601 gtgacattct taaaacattt tgctaagatt cagggatatg attatataaa tgacaagcta
      661 ttttattata atgaacaagt aaaaactctt attgagtact gcattgaaaa tataaaagat
      721 acaaatgtac ttgaagattt aaaaagattt tttggtttta ttgaactaag tattgctaaa
      781 tttcctgtct gcttttatgg ggataaatgg aatattttag tgggagaata tttaaataag
      841 gacttaaata gtgagtataa tgattatata tgtttttatt ctgacactta taataatatg
      901 ttatcagatt ttcaaaaaaa attatatact gttattaaca attctgagat atcttcaata
      961 tgtccaatag aaagttttaa atctatgact gcaaaagaat ttagtttaga taatgagaag
     1021 tatattattc caattgctac aactgaatat aaccaagtta ttgactttaa aattaaaaat
     1081 actaatttta aatcacaaca agtagatcca cctggtttct ttaagtatta tagagtaaat
     1141 aatggcatta gtttaaattc agataaagat gttataattg gtaagccaat aaagctaggg
     1201 tttaaagaaa aaaataaaaa gttagttttc actatattta tagatgcttt atctcaaaaa
     1261 tatttattgg aaaccaaata taaatcaatg ccttacacaa aagctttttt tgacaaagga
     1321 attatatttg aaaattgtta ttcagcagga gagtggacat atccatcttt gacaagtgta
     1381 gttacagggt tatatacaac taatcatcat tttttacatt ctagtgcagg atataaatac
     1441 cctgatgata tagatttgtt cagtgagatt atgaaaaaaa atgaatattt cactgcaaat
     1501 ataagtggta catatgcact tgcaccatat agcagtggtt ttagaggatt tgatagaact
     1561 gtttttaaga atagtttagg attttctgat tcagtaataa ttaatgatgc gattaagcat
     1621 attgaatcat ttaaagaaac caatcaattt gtgtttatgt ctttgagtga tgttcataaa
     1681 atattggatg atattgaaaa ttatagtgtt aattatgata ttgttaatca aacatcttta
     1741 gatatagaga catctttatc aaaaaggagt tcatttgtaa gtgcatggca aaaatatgat
     1801 gaagaggcta ttaaaaaata tgatgtggcg cttagggaat tagaccgtca attgaaagga
     1861 atttatgatt atatattaca aaattactct gaagaagagt atttaattag tattttttca
     1921 gatcatggag cttcattttt aggtaaagat gattttttat taaaatcaaa tataactaat
     1981 agtgtattaa tggttagagg gtctggaagt gcctctaatg gtaatacaga gtatataagt
     2041 aatatggatt taatccctat aattttaaaa ttagcaaata tagaacatga tttatctaag
     2101 tatgattgtg tacttccaaa aacactagga ggtaaggggc gtgagtttac ctatagcgaa
     2161 tcaatatatc ctggtcagac ttataaagct atggtcaata atgataaata ttgttatata
     2221 ttagaaacaa aaggcattac tgatatagat ggaaccatta attttgatga atacagtgac
     2281 tctgtttttg ataaaaaaga agataaagaa gttaaagata atgatat
//
LOCUS       DAEMHW010000326         2292 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.328, whole genome shotgun sequence.
ACCESSION   DAEMHW010000326 DAEMHW010000000
VERSION     DAEMHW010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.328"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            249..1256
                     /gene="asrA"
                     /locus_tag="KPK67_003727"
     CDS             249..1256
                     /gene="asrA"
                     /locus_tag="KPK67_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBG2460637.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNTFEEMEFAKKSNFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            1257..2048
                     /gene="asrB"
                     /locus_tag="KPK67_003728"
     CDS             1257..2048
                     /gene="asrB"
                     /locus_tag="KPK67_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBG2460638.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
BASE COUNT          923 a          210 c          439 g          720 t
ORIGIN      
        1 tcttttttat tcaaaaaatt gagtagatag tagttttatt gtacttaatg attgtattta
       61 agtgattaga tttgaaatcg aataatgtag ataataaaaa aatattaaca gaacttaata
      121 aactccatta atattttttt attatttttg aaactgtgat aattatcacg gaagcataga
      181 aattaaagtg ttaagattta aacaagatag caagtaagta aaactaaaat ataagaaagg
      241 tgataaaaat gaaactaaaa ttagattcat ctaagttcaa tgaaggtctg aattatctaa
      301 aaaaagacta caagatattt gcaccagttt taatcccatt taaaggtact ttttcagata
      361 ctgatgttat aagatacaag gaggtaaaca cttttgaaga aatggaattt gctaagaaat
      421 caaacttttc accaaaagaa gttgtacttc ccataaatca agtattgttt tattttactg
      481 aaaaagagtt taaggaaagc gatttagaca acaagaaaat tttaattttc ttaagagctt
      541 gtgacataaa tggtataaaa agacttgatg aaatatatct aaataatggt gaagaaaaag
      601 attattttta taaaaatatc agagataaag taaagtttgc acttgtaggg tgtaaggaaa
      661 gttttagaaa ctgtttttgt gtgagcatgg attctaacaa aactgataat tattcaattg
      721 gattaaatat agaaggagat acactgtact tagatataaa ggatagtgaa tttgaagtat
      781 ttaatggaga agcaactgaa tttaatgttg attatgtaac tgataattta atatctgtag
      841 atgtgccaga gaatattgat tcaaatgaaa tgataggaaa ttcaatatgg gatgaatatg
      901 atacaagatg tataggatgt ggtagatgta attttgtatg tccaacatgt tcatgtttta
      961 caatgcagga tattttctat aaggaaaacg aaaatgtagg tgagagaaga agggtttggg
     1021 cttcttgtca agtagatgga tatacagata tagcaggtgg aaattctttt agaaaaaaac
     1081 aaggtgaaag aatgagattt aaaactatgc ataaaataca tgattttaaa aaaagattcg
     1141 gatatcatat gtgtgttggt tgtggaagat gtgatgatgc ctgtccgcaa tacatatcat
     1201 tttcaaagtg tatagaaaaa ataaatgatt tagtaattag taaggaggaa gtataaatga
     1261 acccatatat accagtaagt gcaaaaatac tagatatagt taaacatact gataaagaat
     1321 ggacttttag agtaaactgc aatacaaaag gtgtattgcc tggaaagttt tatgagatat
     1381 ctatacctaa atacggtgaa agtccaatat cagtatcagg atatggagag gattatattg
     1441 actttacaat aagaaatgta ggaaaagtaa ctagcgagtt gtttaattat aaagaaggag
     1501 attctttttt tataagaggt ccttatggaa atggatttga tgtatccctt tatgaaggaa
     1561 gagaaattgt agtagttgca ggaggaagtg cattagcccc tgtaagagga atagttgaat
     1621 atttttataa taataaagaa aaatgcgaaa agtttaaatt aatagtagga tttaaatcac
     1681 caaaagatat tttatttgca gatgatttaa agagatggag taaaaaactt gatattttag
     1741 ttactgtaga tggagcagag gaaggatata gtggtaatgt aggattagta acaaaataca
     1801 taccagaatt ggatatgaaa gatataaaca atacttctat agttgtagtt ggacctccaa
     1861 tgatgatgaa atttacagta gaagaatttt taaaaagaga tttagatgaa aagaacattt
     1921 gggtttctta tgaaagaaaa atgtgttgtg gaataggtaa atgtggacat tgtaaaatgg
     1981 atgatactta catatgttta gatggaccag tatttgatta ttcatatgct aaaaatctta
     2041 ttgattaaaa aatagctacg cacactcgtg actttagtca tgagttagta gctaaaatag
     2101 tcagcatata gggaaaccta tatgtagaga taggatagaa cctatccaat actacttgaa
     2161 ttgctggaaa cccctaaagc taatcaaact aaaacataag gatgaaataa acctaagtgt
     2221 gaaagttacg aaagtagaaa aaattggtta gatggtataa ggttaaaccc taagtacttt
     2281 aaaatgggca at
//
LOCUS       DAEMHW010000327         2255 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.329, whole genome shotgun sequence.
ACCESSION   DAEMHW010000327 DAEMHW010000000
VERSION     DAEMHW010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.329"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(102..1669)
                     /locus_tag="KPK67_003729"
                     /pseudo
     CDS             complement(102..1669)
                     /locus_tag="KPK67_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(1644..1787)
                     /locus_tag="KPK67_003730"
     CDS             complement(1644..1787)
                     /locus_tag="KPK67_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460639.1"
                     /translation="MYVYYFESPEKFAFNKVIRTKNQNEISLEKFNEFKETIQDKLLP
                     YNR"
     gene            complement(2005..2187)
                     /locus_tag="KPK67_003731"
     CDS             complement(2005..2187)
                     /locus_tag="KPK67_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460640.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
BASE COUNT          701 a          310 c          252 g          992 t
ORIGIN      
        1 agataatatt taaataaaaa ataccttata aagttaattt tttaatactt tataaggtaa
       61 atacatattg tattgtgggg ataaatttaa atcatttgat actaatcaac actaagaacc
      121 aataactctc tataatcttg agtaattgta tatattctta atttcttgta tgtcataata
      181 ttttctccac ctgtaaaata actcctaaga ttaacacaat cagttttagg ttgatttgta
      241 tctcctaaat aattacgcaa ttctttatct ttagttccaa tatctttaaa tccaaaatat
      301 tgtaaagtag cttcaactct atatttttgg acataatcga gagcttcttt attagtttat
      361 gttggtgtaa aatacatacc atttatataa gcatttattg tacttggatt aaatttaaaa
      421 tctgcagaat atattttatg tgtaactatt atttcttgaa cagttggaat tttaacttct
      481 ggttcatcaa gtatttcagg agtactattt agctctgtaa tagataagtt atctaagtat
      541 attgtatcac tatcaattaa tgttatatct atattagaag aatctttttc agtagtttca
      601 aattcataac taaattttgt atatccttgt tctagtacca aataatccgt tttttcttct
      661 tttactttta tatttacaat tattgaatta tatgttaaag gattttttga attttactaa
      721 aaacataacg tttataaggt tttagttcag acataggtat tttaatcttc gtctcaccat
      781 ttaatctatt tgctgagcct tttaaaccat cttgatttgt agtattaaca ttactccata
      841 tactagggta attattgtaa tcatcaaaat tagtaaagta agttggtgtt tttataagta
      901 tattcattcc tctttcaagt ttaacattac atatttgttt atcagacaaa gtttttaaac
      961 tgtctttaat cttattgcct atattatcat caaatatgag ttcaacacaa ctttcatcta
     1021 ttggtatatt attaaaatat aacaaaccat cttttttagt agtgccaaaa gttttttcaa
     1081 ttgcttctcc aattgtaagt tcaggtgttt tatcttctgg gacctgtaaa cttttagcag
     1141 taattcttct tttgtaagat tcattttctg tatctaatat aatagataca gaaatactgt
     1201 caatttgacc tatatcgtct taccaactat ttcattctgt tactatttgt ccagaaccat
     1261 tttttgtacc aaaatttcca cttacttgtg ttgtttctaa tttaatttgc tttccatcat
     1321 ctaatttttt taattaatta taatttatag gaatcagtct agaactaaat tgatacattg
     1381 tgttaagagc cataggtgaa aatccttttt tgagataagt atttccagga gatagattat
     1441 tgccaatttg attttcttgt gctttgatag ttgataatgc atctccatct aacactaaat
     1501 ttgttgttgg tgtcactttg tacataggtg cagtacctgt gttgtagtat ctaacatttg
     1561 catttatata tgcagattct cctttgttta tacttaatcc agtatttcat gattatccat
     1621 tgctattttg aacagcagtt gaattatctg ttgtatggta ataatttgtc ttgtatagtt
     1681 tctttaaact cattaaattt ttctaatgaa atttcgtttt gattttttgt tcttattact
     1741 ttattaaatg caaatttttc tggagattca aaataatata catacatagg tttatctatt
     1801 ttattttttg atatagcatt tcgaagttct ctatattctt tttctcaaga atttgcttct
     1861 atttgataat cataaaaata atttcttgtc tgagaatatt tgcttatctc tatagaatct
     1921 tttttatatg atcctaatac ttctttttca gatttttcaa gtttttgatc ttttctttct
     1981 cattctttag ccttttcttt atctttaaaa aaatccttct gatctttcta taggagcctt
     2041 gtaagtagta ttacaaactt ttccactata gctttgtaaa tcttgtgcat aaatgttatt
     2101 aggtaacaaa ctacctaaag ttagatataa tatcaacaat atagatatac aattactaat
     2161 acttttatgt ttcctaaatt ttttcattta ttctccctac aaatattagt ttagttaact
     2221 tttgaatttt aatttattta gattatataa tctaa
//
LOCUS       DAEMHW010000328         2236 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.330, whole genome shotgun sequence.
ACCESSION   DAEMHW010000328 DAEMHW010000000
VERSION     DAEMHW010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.330"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            875..1303
                     /locus_tag="KPK67_003732"
                     /pseudo
     CDS             875..1303
                     /locus_tag="KPK67_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1935..2042
                     /locus_tag="KPK67_003733"
     CDS             1935..2042
                     /locus_tag="KPK67_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2460641.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            2044..>2236
                     /locus_tag="KPK67_003734"
     CDS             2044..>2236
                     /locus_tag="KPK67_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2460642.1"
                     /translation="MAVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLK"
BASE COUNT          855 a          273 c          404 g          704 t
ORIGIN      
        1 tttgcccctt actgcgtaac atgagggcat aaaaatagcg gcgttcctgt tctcctgtgt
       61 aggagtgaga aacgccgcct tataggtggt gcttatttta ttttttcttt tcgacgtgcc
      121 gcgtaaggtg gtgtagcggt ctatccggca gctccgcagg tgcttttgca tatcaaggcg
      181 caatccgtta aaagtagtaa atgagtagta aaatcaatct gcaaacctgc gaatatgccc
      241 gtaaatcaag catttttcaa gacaatcaac taatttcata aaaatatatc atagaagtgt
      301 tttgaacatg gaaatattta tgtgcattct ttaaataaaa gtttattttt tgagtgataa
      361 ttaagtgctt gttggtaagg ttatggctgt aaaaatcata actttatttt catatttgtt
      421 caattttcat aaaaattatt atccctttaa gaaaatttct taatcctctt gaaatatttc
      481 cttttcggtc taaagttaaa tttctttctt caaaatgaat atttaaatga actaaaattt
      541 aagtacattt tctaatctta tgagtctatt tttcatagat aattgcccta taatagaaga
      601 atctatgttg gggctatgtg gtgacaataa cagcattgtc agtgattaat taatgcttta
      661 taaatgatta attagtgttt tatgttattt ttggacttac cctctataca tagattaatt
      721 tatttaactt tagatataga gagaatgtca agtacataca cagacattag agtttaagaa
      781 agtgtttatg gagcaagaac ttttaaaaga ggtagtgact tagtttaatt tcaagaaatg
      841 ataaatacaa aagatgaaaa agagaggtgt tgaaatgata gaaaaagtag aagaaaaatc
      901 taagattatt aaaacatcaa aagaatataa gcgaactata attgaagaaa aagataaaga
      961 atggaccaaa aaattaaatg atatacttag tagaagtagt ttttctaatg atgaattaga
     1021 taaaatcaag gcgttaattc caaaagagat tttgactaat gaaaatgttg gagaagttgt
     1081 gactgaagat ggatatgcta agcctgagta tgatgaggta tttaaatcat tgttcacttt
     1141 aaataatgaa tatgatttac ttgtaaattt tataaatgat atcctaaaag atgctccata
     1201 tgctaacagg aatatcaaac cgttcactca aataaagaga ataattagag ttgaaactga
     1261 cccaacaatt aattacattg gagagaaaag acctagactt gattaaaaaa tagctacgca
     1321 cacttgtgac tttagtcatg agttagtagc taaaatagtc agcatatagg gaaacttata
     1381 tgtagagata ggatagaacc tatccaatac tacttgaatt gctaaaaacc cctaaagcta
     1441 accaaactaa aacataagga tgaaataaac ctaagtgtga aagttacgaa agtagaaaaa
     1501 attggttaga tggtataagg ttaaacccta agtactttaa aatgggcaat cagcaggtaa
     1561 gctccgaata ggagaaactt caacgactat tcctcttgag ggaagtacac tacaagctat
     1621 tggtagtgga agtgggtaga ccttaacgga taatgccgaa ggataagata tagtctgtgc
     1681 ttacgtgaaa gtgtaagaag ttcataagag aactgcatag gtagtagcga acttatgtga
     1741 acgacaacct caaaaatgat aaaagttagg acttatggtt cttatatatg ttatttaaaa
     1801 ataaatataa aaaaccttca aaatacttgt tttaagtaat tgcatatttt aatattatga
     1861 taggtgatga aaaatgtaag ttactcatgg tagaggatat gcatatagta tgtcaaatat
     1921 agacatccag tattatgggg agacatcttt ggaatcctag ttatttcgtt gctactgtat
     1981 cagaaaatac agaggagcag ataagaaaat atattaataa tcagaaaaaa caagtaaagt
     2041 gaggtggcag tagtggaaaa agcttataag tttagaatgt atccaaataa aaaacagcaa
     2101 gagttaatta acaaaacttt tggatgttgt agatttgtat ataataagta tcttgctaaa
     2161 agaatagatg tttataaaaa cgataaagaa acttttactt ataaacagtg tagttctgat
     2221 ttgaccaatt taaaaa
//
LOCUS       DAEMHW010000329         2120 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.331, whole genome shotgun sequence.
ACCESSION   DAEMHW010000329 DAEMHW010000000
VERSION     DAEMHW010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.331"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            240..854
                     /locus_tag="KPK67_003735"
     CDS             240..854
                     /locus_tag="KPK67_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG2460643.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            872..1234
                     /locus_tag="KPK67_003736"
     CDS             872..1234
                     /locus_tag="KPK67_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2460644.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1340..1837
                     /locus_tag="KPK67_003737"
     CDS             1340..1837
                     /locus_tag="KPK67_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBG2460645.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          814 a          232 c          339 g          735 t
ORIGIN      
        1 atcatgtaaa atttatataa ctcaattaat tttttaaata caactttata atataaaact
       61 tataataaaa gtaaatttat atgttaaatg aggttgattt tttattattt tttatttatg
      121 ctagtactaa agacttgcaa acgttttttg acaagtcttt attttgtcta aattaaacaa
      181 acttttttac aaataaatac ataaaattta aactaatcta tttattggag gagataaaat
      241 tggaagaagt aattgcaaaa attattacta tagaaaatgg attcaaagaa attgaaaaca
      301 ttgctaaaaa aattgtaaaa aataatgact caaaaaagtg ttattatcta tcaatctact
      361 tatataggtc agaatattat caagtaagag aattggctgt gtttatactt ggttttatat
      421 cagtaactat ttctgaagct ttgttatttt taaaagataa tgtgagtaaa gatgaaaact
      481 ggagagtaca agaaatacta gctaaatcat ttgattatta ttgcagtgaa gtaggatatg
      541 aaaaagcact tcctgttata aaagaatggc ttaattctga tagtccaaac ataaaaaggg
      601 ctgtgacaga aggtcttcga atttggacta gtagagacta ttttaaatct aatcctgacg
      661 ttgctatatc actactttct agtttaaaag aagatgatag cgaatacttg agaaagtctg
      721 taggtaatgc tcttagagac ataagtaaaa aatatccaga tttagtaaaa aaagaattag
      781 attcatggga tatatcaaat aaaaaagttg agtttgtata taagcttgct tcaaaatgta
      841 tcttggcaaa gtaaaatgga ggtgaactta tttgaaactt cgtgatgagt atacttgccc
      901 attagaacta acacatgata tgataaaagg caaatggaaa tctattatat tatggagatt
      961 acgcatagga ccaacttcct tatcaaaact tgaaaaggaa atcaatggta ttaatcaaaa
     1021 gatgttactt gaacaactta aagaattaat tgaatttggt cttgtagata aaaagacata
     1081 tgaaggttac ccacttaaag ttgaatactc acttacacct attcgaggga aacaaatatt
     1141 ggatgctttg gctataatgc aagatatagg aattagcttt atgttggata atggtatgga
     1201 agatattctt aaggaaaaag gtgttttaaa gtaaatagat acccacaaaa aagtaagtaa
     1261 tttaccatta ttttcaactg gtatataatt aaattataga tatattctat taatttatta
     1321 taataggagg taaattatta tgaaagtaac tagtacaggt atagtaaatg gtgttataga
     1381 agataaatac ggtaaaagag gaactcagtt caatgaaggt ggtatgccta catattcact
     1441 acctctaaag tttgaagatg ctccaaagaa tacagtatcc tttgctgtat ttttagaaga
     1501 taaagactct gttcctgtat gtgggtttgc atggatacat tggttagctg taaatataac
     1561 aaaagatgag ttaaatgaaa atgaaagtgt aacagctact gattttgtac aagggactac
     1621 tagttggcat ggaaaactag gaggtcttga cagattagag gccagtgtat atggtggaat
     1681 ggctcctcca gacagacctc atgtctatga aatacatgta tttgcacttg atactatcct
     1741 tgaccttgaa aaaggattct atatgaatga atttttcaaa gctattgaag ggcatgtttt
     1801 agatacattt acactaaaag gaagttattc taattaatta ggttgactat agctaatcat
     1861 taattaaatg atacatttaa ttgatgatta gcttaattgt tattagaata ttttctgcac
     1921 tttatcttaa tatatatttt agttgactgg tattttaatt tctattagat tattatcaat
     1981 ttatataaat tgattttagc ttaaaactta cttatctatt tcagtttcta ttagaattat
     2041 tattagactc attaaaataa aagcactaat ataactaata atatatgtta aattcattaa
     2101 ctctacataa agttttataa
//
LOCUS       DAEMHW010000330         2066 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.332, whole genome shotgun sequence.
ACCESSION   DAEMHW010000330 DAEMHW010000000
VERSION     DAEMHW010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.332"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..129
                     /locus_tag="KPK67_003738"
     CDS             <1..129
                     /locus_tag="KPK67_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2460646.1"
                     /translation="EVKDLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            247..924
                     /locus_tag="KPK67_003739"
                     /pseudo
     CDS             247..924
                     /locus_tag="KPK67_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
     gene            944..1495
                     /locus_tag="KPK67_003740"
     CDS             944..1495
                     /locus_tag="KPK67_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG2460647.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            1659..>2066
                     /locus_tag="KPK67_003741"
     CDS             1659..>2066
                     /locus_tag="KPK67_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG2460648.1"
                     /translation="MIIKVSITVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIIQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYS"
BASE COUNT          854 a          185 c          369 g          658 t
ORIGIN      
        1 gaagttaagg acttaaatat aagagaatgg atttgtccta gttgtaatga aactcacgat
       61 agagatataa atgcaagtat aaatatatta aaagaaggat taagactaat aacaattcaa
      121 aataaataac gtatgtaaga accgtaggaa ctacggggat agcctgtgga gaattagtaa
      181 gacatatttt agtatgcaaa attctatgaa gcaggaaccc tgtgacttta gtcatgggag
      241 gttcagagaa cgcaaataaa taataaatgt gatttatgtg gagagtgtat caaggtatgt
      301 cttaaagatg ccatttgtat aatgaccgaa gaaatgagtg ttgaagaaat agtagaggaa
      361 gttgaaaaag attttatatt tttctttgaa tctaatggag ggataacttt ttctggaggt
      421 gaacctacac ttcagattga ttttttaaga gaattagttg atatttttta tgataaaggc
      481 ataaatattg caattgaaac ttgtggatat tttgattgga ataaggtaaa tgatgtattt
      541 gagaaaatag accacatatt tgtagatata aaatctatgg atgataatat tcataaagaa
      601 tatacaggag taagtaataa aattatatta gataatatat gtaggctttc aaagttgaat
      661 aaatctatgg ttataagagt gccaattatt tatggagtaa atgatagtga agaaaatata
      721 agaaatactg cattatttgt caaacaaaat gtcccaggag gcaagatgga gttacttcca
      781 tatcataaat ttggaatcga taaatataag gcattagggc ttgaagatta tatatatgaa
      841 tttgatgaaa tttgtaacaa tcatatgtta aaattaaaag agatagtaga acttacagga
      901 gtaaaaatca tagaatacaa atagctatca ggaggatttt aacatggata ataaatttcc
      961 taatgtggga gaaaatttaa ggttattgag acaagaaatg ggcataagtt tagacaaagc
     1021 ttcaaagatg acaggtgtca gtaaagcaat gcttggacag attgagagag gtgaatcaag
     1081 tccgacagta tctactttat ggaaaatttc atctggattt aaaataaatt ttactacact
     1141 attaaatgaa aatacaaata cctatgaggt tataaaaaaa gaagaagtag agcctattgt
     1201 tgagcaaaaa gggaacatga agttatatcc aatataccct tttagccctc aaagaaggtt
     1261 tgaacttttt ataattgagc ttgaggaaaa ctgtactcat gtttcatcta cacatagtca
     1321 tgttttagaa gagtatgtac tcgtaataga aggtaaactt gaccttaatg tggatgggaa
     1381 aacttatatt ttagagtctg gacattctat taggtttgat ggaactttag agcatatata
     1441 taaaaattta aataaaggaa aaactatatt tcacaatata gaggtttata gataatttta
     1501 tagttatcct tttctaaaat aaatgaaaat tttagaaaag gataacttat ttttataatg
     1561 tcgaaaagat ttatatgtat gtgatgatgt tattgtttta aaaaaaacat aaaagcatta
     1621 taataatatt aacaaaatag aataaagagg aagagaatat gattataaag gtttcaataa
     1681 cagtattagt attaatatta ttgtatattc tgtataagta tgaaactcag catatagaaa
     1741 cgacagaata cactattttc aacaaaaaaa ttccaaagga atttgataac tttaaaataa
     1801 ttcaagtgag tgatttacat aataaggtgt ttgataaaaa taataaggta ttgatagata
     1861 aaattgaatc attaaaccca gatgttatat ttataacagg agatttagta gatggagaaa
     1921 ataaaaattt tcaagttgca ttagatttaa tagataattt ggttgaaaaa tatgaagtat
     1981 atcatataat tggaaatcat gaacaaaaat ctttaataaa aaagtataaa catctatata
     2041 aggattattt taaggaacta tattca
//
LOCUS       DAEMHW010000331         2044 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.333, whole genome shotgun sequence.
ACCESSION   DAEMHW010000331 DAEMHW010000000
VERSION     DAEMHW010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.333"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            471..1799
                     /locus_tag="KPK67_003742"
     CDS             471..1799
                     /locus_tag="KPK67_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG2460649.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIIIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGDGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          730 a          224 c          348 g          742 t
ORIGIN      
        1 aatattagtt ttgatttctg gtttaaattg ggttataata aattgtcaaa ttatttatta
       61 atataaaata gactataaaa caagcaaaaa tcatactatt atataaaaga ccttaaagat
      121 agaaaactac agaaagaagc caaaatgttt ttgaaaaaaa tgaaatttta ttgcaaaaaa
      181 atcaaaatga atttaactta atagttataa tctattttta gtgttacaaa tatacaatgt
      241 atattataca atataaaaaa tacatttact tagaagtatt attatatttg gatttttggt
      301 ttttataatt tttttatatt taaacataac taaaaataaa agattactaa agctaagtag
      361 agtttatttt attttaataa ataataaagc tatttttcta tatattggca taatatatgc
      421 ttatttaact atgaaattaa aattaaatag gattaatgag gaggaaaaaa atgggaagta
      481 atgaactgaa aaagactctt ggcgtatcag cagcattatc aactgttgtt ggttccgtta
      541 taggagcggg cgtatttttt aaaccacaag cagtctatac attaactgga ggagctccag
      601 gtcttggtat attggcatgg ttaatagctg gaatcataac tataacagct ggtcttacag
      661 ctgcagaggt atcagttgcg ataccaaaaa ctggtggaat gatggtatat ataaaagaaa
      721 tttatggaga aaaattaggt tttttaactg gatggatgca aattgtatta ttttatccag
      781 ggatgatggc agcattaggt gttatatttg gagaacaagc atcagcacta attggtagtc
      841 catcactttt acttccaata gcaattggaa ttataataat agttgcaggg cttaatatgt
      901 taggttcaaa aactggagga gtcatacaaa ctgtttctac aatttgtaag ttaataccac
      961 ttattttaat aatggttgtt ggatttatta aaggtgatgg aaataatcca atacttacac
     1021 ctatggttgg tgaaggatta agcctgggta gtgtactagg tcaagtttta attgctatat
     1081 tatttgcttt tgatggatgg atgaatgttg gtactttagc tggagaaatg aagaatcctg
     1141 gaaaagacct tccaaaagct ataataggtg gattgtcagt tgttatggct gtttatttta
     1201 taataaattt agcgtatctt tgggtattac cagctaatga attagcaaat tatgcttctc
     1261 ctgcatcagc agttgctgaa gttatatttg gttcaatggg tggaaaaata atatctgtag
     1321 gtatattaat ttcagtgttt ggagcattaa atggattttt actaactggt tcaagggtgg
     1381 catatacttt agctacagat aagactttac caaagtatgg tatattttct aaattaaatt
     1441 ctgctcaagt tccagcaaat gcaatagcat tagtatctgt tattgcatct atatatgctt
     1501 tgtcaggtca atttaattta ttaactgact tagctgtatt tgctacttgg atattctatg
     1561 tattaacttt tataggggtt atgaaactta gaaagactca tccaaatata ccaagagagt
     1621 ataaagttcc attatatcct attgttccta taatagctat agcaagtggt atatttgttg
     1681 tagttaatca attgtgtttt gcaggtatga aaactactat gatatctata ggaggtcttg
     1741 taataacagc tataggctta ccagtgtatg cttatatgac tagaggagta aaaagataaa
     1801 ccaagtagtt tacaaatgct tcataattaa aataaaagag actgtctcaa atagagataa
     1861 gtctcttttt tattgttaaa atggtaaaga tatgtttgat tttacaaaaa aaagagcgtt
     1921 ccatgaacta aaaagtttat tttaaggtat tcattttatt tttagttttt tgattataaa
     1981 attaaacacc taaaaaatta tttattttaa aatactttat tttaaagtgt tttttatcaa
     2041 acta
//
LOCUS       DAEMHW010000332         2043 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.334, whole genome shotgun sequence.
ACCESSION   DAEMHW010000332 DAEMHW010000000
VERSION     DAEMHW010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.334"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..906
                     /locus_tag="KPK67_003743"
     CDS             <1..906
                     /locus_tag="KPK67_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG2460650.1"
                     /translation="NIKKEKKSISKIVFNLVLILLISYIESVAFSSHINGMFNTSFSF
                     YTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEIDVQETKDGELIVLHDSNFKRTTG
                     VDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVGEKIPTLKEMIKYSKGKVKLLIEI
                     KLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKTILQKVKKLDENLLTCYFMAIAYG
                     DFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFVWTVNSDSLIEKMLDLNVDSIITD
                     NPYLIESAIYWKKNGFISQVSDYLF"
     gene            1228..1812
                     /locus_tag="KPK67_003744"
     CDS             1228..1812
                     /locus_tag="KPK67_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG2460651.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT          800 a          206 c          338 g          699 t
ORIGIN      
        1 aatattaaaa aagagaaaaa atctatatca aaaattgttt ttaatttagt attaatatta
       61 ttaatttctt atatagagtc agttgcattt tcttcacata taaatggtat gtttaatacg
      121 tcattttcat tttatacaac agcaattgct catagagcag gagctgaaat ggctccagag
      181 aatactttgg cagcaataga agaagcttca catgctaaag ctgattatgc agaaatagat
      241 gttcaagaaa ctaaagatgg agaacttatt gtacttcatg attcaaactt taagagaact
      301 acaggagtag ataaaaatgt ttgggatgta aattataatg aagtaaagac atatgatgcg
      361 ggaagctttt attattatgg aaaaggttat tatgatgaaa agtttgttgg ggaaaagata
      421 ccaacactca aagaaatgat aaaatattct aaaggtaagg tgaaattatt aatagaaata
      481 aaacttaatg gacatgaaaa gggagatgta gtaaaaaaag ttgttcagtt aattaaagaa
      541 aataattttg aaaatcaatg tatagttgca tctatggata aaactatatt acaaaaagta
      601 aaaaaactgg atgagaattt actgacatgc tattttatgg caattgcata tggtgatttt
      661 tcaaagttaa attatgttga tatatatgga attgaatcaa cttttgttaa taaaacagta
      721 gtagaaaaga ttcataaaat gaacaaaaaa atcttcgttt ggactgttaa tagtgacagt
      781 ttaattgaga aaatgcttga cctaaatgta gatagtatta ttacagataa tccatacctt
      841 atagagagtg caatttattg gaagaaaaat ggatttatta gtcaagtgtc agattattta
      901 ttttaaatat tctataattg tattatccag taaaagagat attattaaaa tcaataatat
      961 ctctttttaa cataaataag gttgttaaat taaggttatt cttataaaaa ttgtgcttat
     1021 cagctataaa caaggaataa aagtgaaaaa attgctctga gattattaca gttaaaagag
     1081 ttttaagatg acagagagat agattgtcaa ctgtatattt aataaatctt gttatttatt
     1141 aaattttata taagtaaaat atataaatta ataaatctta tgctagaata tatatgaaaa
     1201 acaatataaa tttggaggat taaaaacatg acagagcgag aaaaaatgtt ggcaggagaa
     1261 ctttacgatt gtggtgatat tgagttgtta actcaatggc ataaagcaaa agacctagta
     1321 agagattata accaaacaga ttcctcggat ttggaaaaaa aagaaaaaat attaaatgag
     1381 ttacttggtg gtaagggtaa aaacttatgg attactgcac cgtattacgt agattatgga
     1441 aataatattt attttggaaa taattgtgaa gtcaatatga attgtacgtt tttagatgat
     1501 aataaaatta tcattgggga taatgcacta atcgcaccaa acgtacaaat atatactgca
     1561 tttcatccaa ctaatgctgg tgatagattt ggtaatgcca aagaagatgg ttcatttgaa
     1621 ttttgtaaaa ctcaaacagc tccagttact attggagata atgtttggat tggcgggggt
     1681 gctattatca tgccaggtgt tactattgga gataatgtag ttataggtgc aggaagtatt
     1741 gttacaaaag atattccaag taatatgatt gcatacggaa atccatgccg tattattcga
     1801 gaaaataaat aaagttaaat tttcttgcaa aatctcaaaa aacacctttc ttactttata
     1861 catggggtat tttctaatac tttcatatcc atattcattt gttaaatttt tgtgtaatgt
     1921 agtttttatc acttctatct tacatttcca ccattttttt ataaaagtca ttcctttata
     1981 gatttattta taatagtata tattctattt gatgtttgaa taagcactta tttttaagta
     2041 aaa
//
LOCUS       DAEMHW010000333         1983 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.335, whole genome shotgun sequence.
ACCESSION   DAEMHW010000333 DAEMHW010000000
VERSION     DAEMHW010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.335"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            52..1266
                     /gene="dpaL"
                     /locus_tag="KPK67_003745"
     CDS             52..1266
                     /gene="dpaL"
                     /locus_tag="KPK67_003745"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG2460652.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            1393..>1983
                     /locus_tag="KPK67_003746"
     CDS             1393..>1983
                     /locus_tag="KPK67_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG2460653.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT          771 a          213 c          432 g          567 t
ORIGIN      
        1 tgcttatata ttaaagtata aaaatttaac aataatttgg aggtatttta gatgttaaaa
       61 gaaattaaat ggaaagtaaa taatttgcca aagggcgata aggaaaattg tatcaaattt
      121 ttaaatgaag aagaaataac taaggttaga aattttcaca aaagctttcc tcaatataaa
      181 gaaactccat tagcaaatct agaaggttta gcaaaaaaat taggtgttgc aggagtatat
      241 gtaaaagatg aatcttatag atttggatta aatgcattta aagtattagg tggttcttat
      301 tctatgggta gatacttagc tcaaagatta gatacagata tatctgagtt agggtatgac
      361 aaattgactt cgaaagaaat aaaagaaaaa ttaggagaaa taactttctt tacagctaca
      421 gatggaaatc acggtagagg agttgcttgg acagctaata aattaggtca aaaatctgtt
      481 gtattaatgc caaaaggttc ttctgaattt agattaaata aaattaaagg agaaggtgca
      541 gatgctagta taacagactt aaactatgat gatgcagtaa gattagctaa tgattatgca
      601 gaagctgatg accatggagt aatggttcaa gatacagcat gggacggata tgaagaaata
      661 ccagcgtgga taatgcaagg atatggaact atggctcaag aagctataga gcaattaaaa
      721 gaatatggag tagatagacc aacacacgta tttgttcaag caggagttgg ttcacttgct
      781 ggtgcagttc aagggtatgt tgcttcaata tatgatgaat gcccaataac agtagtagta
      841 gaggctgatg aagctgattg ttactataaa tcagcagaag ctggagatgg aaaaccaaga
      901 tttgttggag gagatatgcc aactataatg gcagggcttg cttgtggaga accaaatact
      961 ataggatttg aagtattaaa aaatcatgca gcagcatttg tttcagcacc agactgggtt
     1021 tctgctaaag gtatgagaac attaggtaat cctttaaatg gtgatgaaaa agtaatttct
     1081 ggagaatctg gagcagttac aactggtctt ttagtagcag ctatggaaag agaagattta
     1141 gcagatttaa gaaaagattt aaaattagat gaaaattcaa gaatactttt aataagcaca
     1201 gaaggagata ctgaccctga caaatataga agtatagttt gggatggaga atatccaagt
     1261 atataaggaa ttactaaaat gcaattatta gatattggtt tacatattta gaagatatat
     1321 aagttctaaa atttagaaaa tatataagta tataaaatac atacattata aaaaatctag
     1381 ggggataatg aaatgttaaa cgaacaaaga aaacaagaag taactgaaat atgccaaaaa
     1441 ttaattcaaa atcctagttc ttctggaaat gaagaaggtg ttgtaaaagc aatagaagag
     1501 tcttttgaaa aattaggttt tgatagttgg tcaagagata gatatggaaa tatagtagga
     1561 tgtataaaag gtaacaagcc aggtaagaaa atattatttg atggacatat agatacagtt
     1621 ccagttccag atgcaagtaa atggagtgtt cctccttttg aaggaaaaat agttgatgga
     1681 aagatatatg gtagaggaac ttctgacatg aaaggtcaat atactgcaat gatgtctgct
     1741 gtagcgtatt ttgctaaaga cactaataga gattttgctg gagaactata tgtagcaggt
     1801 gttgttcatg aagaaatatt tgaaggtgtt tcggctagag agataagtaa ggctgttcaa
     1861 cctgactatg ttgtaatagg tgagtcttca gagttaaact taaagattgg tcagagaggt
     1921 agaggagaaa tagtagtaga aacttttggt aaaccagctc actctgctaa tccagaaaaa
     1981 ggt
//
LOCUS       DAEMHW010000334         1969 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.336, whole genome shotgun sequence.
ACCESSION   DAEMHW010000334 DAEMHW010000000
VERSION     DAEMHW010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.336"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(29..607)
                     /locus_tag="KPK67_003747"
     CDS             complement(29..607)
                     /locus_tag="KPK67_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2460654.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(665..1381)
                     /locus_tag="KPK67_003748"
     CDS             complement(665..1381)
                     /locus_tag="KPK67_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG2460655.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            1572..>1969
                     /locus_tag="KPK67_003749"
     CDS             1572..>1969
                     /locus_tag="KPK67_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG2460656.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTL"
BASE COUNT          578 a          321 c          183 g          887 t
ORIGIN      
        1 acaatcttgt caagtttttt agtatttatt atgctttttt cttttcaatt aaaatcaatt
       61 gtggtgggtt attgacttga tttaaaaact tagattctaa aacattaaat tcatcttgat
      121 ttaaattatt gacaaggtta tatatgcaat ctttttcttc ttgacctcca tcatgccctg
      181 tatatatggc aatgcttatt actccatttt cttttaacat ttctaaactt aactttatag
      241 cctttaaagt tgtatctggt ttggttataa ttgaatgttt cgctcttgga agataaccca
      301 aattaaataa aacacatgaa acttcttctt ttatatattg attcattttt tcatgcccat
      361 caaatatcaa ttttactcta tctgtatatc cttctttctc tagtttcttt cttgtagact
      421 ttattgcttc ttcttgtata tcaaaagcat atacaaagcc cttttctcca accttctcag
      481 ctagatacct tgtatcatac ccatttccca tagtagcatc aattactatg tcaccttcat
      541 ttataatatt ttctaaataa aatttattta tatctgtaat tttagttaaa tactttgcat
      601 atgccataat actatttctc cctactatta actttatttc attttttctc aatttatttt
      661 tatcttattt tattgatttt aaatatttta tttcatcttc tgttaggtat ctatattctc
      721 caactttggt gtcttttaat actattttcc ccacagaaat ccttcttaaa tttaaaactg
      781 gatgatttat agctttgcac atttttctta cttgtctatt tctaccctca tgtatagtta
      841 tttcacacac tgaataattt ttttctttat tttctttagt tacccttatt ttggcaggtg
      901 ccgttgtgta atcttctatt tttagtcctg tttcgaattg tcttatctca tcatcactta
      961 taattccctt cacacttgcc acatacgttt tatcaatttc atgctttgga tgggtaagct
     1021 tatatgttaa atcgccatca ttagtaagta ttaataaacc actagtttca taatctaatc
     1081 tccctattgg atatactctt tcccttacat ctttaagtat gtctattaca ctgggtctat
     1141 tgaattggtc ttttactgta gttatatatc cctctggttt atttaatact atatatatat
     1201 aattttcact tggttttact tttttattat caaattctac tatgtctttt tcttcatcaa
     1261 tattaaaaga taactctttt actacacttc catttacctt tattcttcct ccaagaatta
     1321 tttcttctgc ttttcttctt gatgctattc cacaagaagc tatatactta ttaattctca
     1381 tacttaatcc ttccttatct taaattatgt gtgtttaata tttttatcaa agttaatttt
     1441 gctacattat tatactatac tagaccttaa ttaaaaacaa gcaaaaaaat tatttaattt
     1501 tcctaaataa ttgtaatttc ttttcacata ctattaatat gtaatagctg actaaacact
     1561 attttaagga agtgattatt atttcaaagg aaaatataaa atattattta atagtcgtgt
     1621 ttattggaat tttatttttt aaatttatta atactccatc agactttata tctagtattg
     1681 aaggattctt aaagtttttt agcccatttt tattagctat tttacttgct ttacttttaa
     1741 atccacttgt gatgcttttt gaggtaaaat tcaaagcaca tagactttta gccatttttt
     1801 tatcttatat atttataggt tttattttag cctttgccat taggttatta attccatcaa
     1861 tagctaatac attaaataga ttaataaatg aaatgcctat gtatactgat tatatagata
     1921 gttttataga aaaaaatatg tcaaacatag attttctaaa aactttaat
//
LOCUS       DAEMHW010000335         1961 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.337, whole genome shotgun sequence.
ACCESSION   DAEMHW010000335 DAEMHW010000000
VERSION     DAEMHW010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.337"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            223..507
                     /locus_tag="KPK67_003750"
     CDS             223..507
                     /locus_tag="KPK67_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460657.1"
                     /translation="MLVYNKSFYPNDIFPRLDFLKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(1278..1493)
                     /locus_tag="KPK67_003751"
     CDS             complement(1278..1493)
                     /locus_tag="KPK67_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2460658.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1504..>1961)
                     /locus_tag="KPK67_003752"
     CDS             complement(1504..>1961)
                     /locus_tag="KPK67_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG2460659.1"
                     /translation="FFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKVIALI
                     ARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGCCLLINTIIECINGYNLN
                     TNNIRIFGTDSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          793 a          202 c          244 g          722 t
ORIGIN      
        1 agatagtagc tatttttata tagctattat ttttttattg ttgtggatga aaatatactt
       61 atattttact aatatacatt taaaaattac aatttaaagc attaaaaaat aaatttgtat
      121 tttgttttat ataaagtaat ttgaaaaata tggaaaaatt gattagtaca ttattaatta
      181 gggtataaaa tggtgtaaat agaatgataa ggggtggatg aaatgcttgt atataataaa
      241 agtttttatc ctaatgacat atttccaaga ttagattttt taaaaataaa aaaacagtta
      301 aaattgatag ataatgacct gtcagatttt ggaagcatat gtataataga aaaagaacat
      361 tatacgataa gtgtaaacag tataggtgaa ataaatgtgt attatgattt agagtacgaa
      421 aataaggtgt atagaatagt ttatgagatt gaaaagttat ttaaatctca agttggaagg
      481 tttagtatat ctacatacag aaattgataa ttaaaaaagt agaaattaaa aaacttaata
      541 ctaaagatat agataaatat atgaggtaac agctagagag agttgttacc ctttttaaat
      601 actaagtata tattattctt attaattttt tgtaaattca ctaaaagacc tttttataga
      661 atattatttt ctttttttat taaataggta aatcagtaaa aatgcaattc cagttccaat
      721 tattattcca atgatatttt ctataattaa tttttttaat ataaaagtaa ataataaaaa
      781 tgctatacat aaacctaaaa tatatccttt aaaataggtt gaaagttttc ttttcatata
      841 atcaccaact tgtatcagat ttagtttact taattagagt ataacagtgt tgtaacttca
      901 aagagtatct aaaagattac aaatgttaga taaaatcatc aataatgtaa tttataacac
      961 gtagctataa actatgcaat aatataaatg ggggtataag taaatgaaaa agttccatgt
     1021 tttgctaaca gtttaaataa atattaggta gaaaagaatg tatataggaa tctgtggtaa
     1081 aattatcaat ttaaaagtat tatttagtgt gatacagttc ttataggtaa tggataacaa
     1141 atagataaag gaacagtttc tttcttagaa catattttgt tatgacttta aacaaagtta
     1201 atattaatgt caataatata gtaaaagaat aaaaatcata tagtataata aaaaagagtg
     1261 tttctaaaca ctctttttta atcattttca tcttctacat attcaagtat atcacctggc
     1321 tgacagtcta aagctttgca aatttcgttt agagtggtaa atctaatagc ttttgctttt
     1381 ccagttttta aaattgataa atttgcattt gtaatatcta ttttttctgc caaatcttta
     1441 agtgatattt tcctttttgc catcattaca tctaggttaa ctactattgc cataacatca
     1501 cctctatatc gttaaatcat tatcttcttt tatttttata gctttatcaa atgtttctgc
     1561 aattacaaag cacattagtg caaatataaa agagataacc ataagtggtg agataccgcc
     1621 actatcagtt ccaaaaattc ttatattatt agtatttaaa ttataaccat tgatacattc
     1681 aattatagtg tttatcaata aacaacatcc cattacttta aaccttttta cattttcaag
     1741 tacgaatggg gaatacttag tactattaat tataaatatc aagaataaga tgactataaa
     1801 gtagtcagaa aaatatagta ttcttgcaat taaagcaatt actttgtcag aactaaaatc
     1861 atataaaaac ataggtatca tacataagaa tattatacca gcaactccta aaagtgataa
     1921 tagaaaatat agaaatactg ataatatatt actaaaaaat t
//
LOCUS       DAEMHW010000336         1960 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.338, whole genome shotgun sequence.
ACCESSION   DAEMHW010000336 DAEMHW010000000
VERSION     DAEMHW010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.338"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            57..173
                     /gene="rrf"
                     /locus_tag="KPK67_003753"
     rRNA            57..173
                     /gene="rrf"
                     /locus_tag="KPK67_003753"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            180..254
                     /locus_tag="KPK67_003754"
     tRNA            180..254
                     /locus_tag="KPK67_003754"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:212..214,aa:Asn,seq:gtt)
     gene            259..333
                     /locus_tag="KPK67_003755"
     tRNA            259..333
                     /locus_tag="KPK67_003755"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:292..294,aa:Glu,seq:ttc)
     gene            339..414
                     /locus_tag="KPK67_003756"
     tRNA            339..414
                     /locus_tag="KPK67_003756"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:372..374,aa:Val,seq:tac)
     gene            420..496
                     /locus_tag="KPK67_003757"
     tRNA            420..496
                     /locus_tag="KPK67_003757"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:454..456,aa:Asp,seq:gtc)
     gene            507..581
                     /locus_tag="KPK67_003758"
     tRNA            507..581
                     /locus_tag="KPK67_003758"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:539..541,aa:Thr,seq:tgt)
     gene            596..680
                     /locus_tag="KPK67_003759"
     tRNA            596..680
                     /locus_tag="KPK67_003759"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:630..632,aa:Tyr,seq:gta)
     gene            690..763
                     /locus_tag="KPK67_003760"
     tRNA            690..763
                     /locus_tag="KPK67_003760"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:722..724,aa:Gly,seq:tcc)
     gene            774..850
                     /locus_tag="KPK67_003761"
     tRNA            774..850
                     /locus_tag="KPK67_003761"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Arg,seq:tct)
     gene            860..935
                     /locus_tag="KPK67_003762"
     tRNA            860..935
                     /locus_tag="KPK67_003762"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Gln,seq:ttg)
     gene            947..1022
                     /locus_tag="KPK67_003763"
     tRNA            947..1022
                     /locus_tag="KPK67_003763"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Lys,seq:ttt)
     gene            1025..1113
                     /locus_tag="KPK67_003764"
     tRNA            1025..1113
                     /locus_tag="KPK67_003764"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1059..1061,aa:Ser,seq:tga)
     gene            1131..1221
                     /locus_tag="KPK67_003765"
     tRNA            1131..1221
                     /locus_tag="KPK67_003765"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1165..1167,aa:Ser,seq:gct)
     gene            1230..1306
                     /locus_tag="KPK67_003766"
     tRNA            1230..1306
                     /locus_tag="KPK67_003766"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1264..1266,aa:Pro,seq:tgg)
     gene            1392..1467
                     /locus_tag="KPK67_003767"
     tRNA            1392..1467
                     /locus_tag="KPK67_003767"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1425..1427,aa:Trp,seq:cca)
     gene            1528..1604
                     /locus_tag="KPK67_003768"
     tRNA            1528..1604
                     /locus_tag="KPK67_003768"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1562..1564,aa:Pro,seq:tgg)
     gene            1611..1687
                     /locus_tag="KPK67_003769"
     tRNA            1611..1687
                     /locus_tag="KPK67_003769"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1645..1647,aa:Ile,seq:gat)
     gene            1691..1766
                     /locus_tag="KPK67_003770"
     tRNA            1691..1766
                     /locus_tag="KPK67_003770"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1724..1726,aa:Phe,seq:gaa)
     gene            1774..1850
                     /locus_tag="KPK67_003771"
     tRNA            1774..1850
                     /locus_tag="KPK67_003771"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1808..1810,aa:Met,seq:cat)
BASE COUNT          500 a          449 c          530 g          481 t
ORIGIN      
        1 tgacaaaaat agtaaaaaat gttagtatta tacttgtcct agcaacaaga aaaaaatgtg
       61 gttataatag cggagaagat acacctgttt ccattccgaa cacagaagtt aagttctcta
      121 gcgctgatgg tacttggtgg gaaactgcct gggagagtag gacatagcca cgtgaatgtt
      181 ccaaggtagc tcaacggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc
      241 cacccttgga gccattatgg ctcgttggtc aagaggttaa gacaccgccc tttcacggcg
      301 gtaacagggg ttcaattccc ctacgagtca ccatttgtgg gatcatagct cagctgggag
      361 agcacctgcc ttacaagcag ggggtcacag gttcgagccc tgttggtccc accatttgcg
      421 gcctggtagt tcagttggtt agaatgccag cctgtcacgc tggaggtcga gggttcgagc
      481 cccttccagg tcgccaaaat aaaaatgctg gtgtggctca acggtagagc agctgacttg
      541 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaact aatacggagg
      601 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      661 gaatccacca tcctccacca attaaatatg cgggtgtagc tcaatggtag agttctggcc
      721 ttccaagcca gctgtgaggg ttcgatcccc ttcacccgct ccaaagaaag cgtgggtgca
      781 tagctcagct ggatagagca acgcccttct aaggcgtgtg tccggggttc gaatccctgt
      841 gcgctcacca catatatatt ggggattagc caagtcggta aggcacatga ctttgactca
      901 tgtatgcgta ggttcgagtc ctgcatcccc agccaaataa aaatatggtc tattagctca
      961 gtcggtagag cacctgactt ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac
     1021 caatggagag gtgtccgagt ggtttaagga gctggtcttg aaaaccagtg acttcgaaag
     1081 gggccgtggg ttcgaatccc accctctccg ccaaaaaaat gactgaattt ggagaagtac
     1141 tcaagtggct caagaggatc ccctgctaag ggattaggct gggtaaccgg tgcgagggtt
     1201 cgaatccctc cttctccgcc attcaaaaac gaggtgtagc gcagtttggt agcgcacatg
     1261 gtttgggacc atggggccgg gggttcgagt cccttcacct cgaccaactt aagcttttag
     1321 ttcagttggt tagagtgccc aactcatggc tagtaattct agggtttgag tccatgaagg
     1381 ttattcttca taggggtgta gctcagttgg tagagcgtcg gtctccaaaa ccgtgcgccg
     1441 ggggttcgag tccctccacc cctgccaaat aaaaaggact tgaaaaaagt tggattttag
     1501 agtagtataa gatagtaaat aagatttcga ggtgtagcgc agtttggtag cgcacatggt
     1561 ttgggaccat ggggtcgggg gttcgagtcc cttcacctcg accaagaaaa gggcctatag
     1621 ctcaggtggt tagagcgcac gcctgataag cgtgaggtcg ctggttcgag tccagctagg
     1681 cccaccattt gcccagatag ctcagtcggt agagcagggg actgaaaatc cccgtgtcgg
     1741 tggttcgatt ccgcctctgg gcaccaggaa tacggcggta tagcttagtt ggctagagcg
     1801 ttcggttcat acccgaaagg tcataggttc gactcctatt accgctacca tctaactata
     1861 tcaataaaga actttgaaaa ttaaacagta ggttaattta taaaacaaga aacaaaccat
     1921 aaagccagat attttgataa caatagtatc tgagcctgat
//
LOCUS       DAEMHW010000337         1937 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.339, whole genome shotgun sequence.
ACCESSION   DAEMHW010000337 DAEMHW010000000
VERSION     DAEMHW010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.339"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            342..1634
                     /gene="brnQ"
                     /locus_tag="KPK67_003772"
     CDS             342..1634
                     /gene="brnQ"
                     /locus_tag="KPK67_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG2460660.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCVMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          732 a          221 c          316 g          668 t
ORIGIN      
        1 ggagagtgat atatacagta tataaagcaa tacaacttaa taataaatcg aataatctaa
       61 aaagtattaa tattttaatt attcggaata tttaatttta tcaagaaaaa ttagagaaaa
      121 aacaaaaaac caactatgtt taaagtatac aggttgattt tgtttaatta agaaagttgt
      181 attaaatatt gtgaagattt tataaattat gaaacataat tgacaaattt aagtcaaaaa
      241 tgtataatta aataaaatct cgtcaaaagg ttttcattta attaagagat tggacttatt
      301 ttttagtaaa aggtagtaaa attcaaatgg gggtgcttta aatgggtgag aaacaaatca
      361 attttaaaga tgtaattgta attggatttg cactatttgc aatgtttttt ggtgcaggga
      421 acttaatctt ccctccatat ctaggagttt tatctggaag tagttggcta gtagctttca
      481 taggattcct ttttgcagat ggtggtttgg cattattagc agttatagca gcaactaagt
      541 tcaatggaga cacatcgaag atgttctcaa gagcaggcaa aggattaagt attgcattag
      601 gatgtgtaat ggtaatctgt atagggccac ttttagcaat acctagaaca gcagcaacaa
      661 cttatgaaat gggtatatta cctacaatag gttctggaat aagcccagta atattctcta
      721 tagtattctt tgctctagta ttggttttaa caataagacc atcaaaagtt gtagatatag
      781 ttgggtcaat attaactcca gctttactta tagctttagc agtattaata gttaaaggta
      841 tcgtatctcc attaggagaa ataagagaca gttctttaat acaaaatgta tttgcagaag
      901 gtataactca aggttatcaa acaatggatg cattggcagc tagtgtattt gcatcaatta
      961 taatcatgtc agtaatagct aaaggttata caggtgagaa agaaaagatg aaagccacaa
     1021 taagtgcagg tgtaattgca gtaataggaa tggcattagt ttatggtgga ttatgttatc
     1081 ttggagcaac tgtgtctcaa caatatggac aagatgttca acaaacagct ttaatagttg
     1141 caataacagc agctttactt ggaactacag gtaaaatttt attggctatt atagttgcat
     1201 tagcatgttt aacaacagct ataggtcttt cttcagcagc tggtcaatat ttctcaacac
     1261 ttacagatgg aaaattaaag tatgagcata tagttatagt agtttgtgta tttagtgcaa
     1321 tcatatcaaa ctttggagta agtacaataa tacaattctc ttctcctata ctaagtatgg
     1381 tatatccagc aactataact ctagttatac tagcattatt tagtaataaa attaaaaatg
     1441 acaacgtgtt caaatgtgct gcatacatgg cattattagt aagtgtatta acagttgcaa
     1501 caagttttgg tgtaaaaatt cctctagtta atagcttacc atttgcttca ttaggattta
     1561 actggatagt tccagttgta atagcaggaa taataggaaa ctttattcca tctaaaagca
     1621 gtaaaactgc ataataataa aaactaattg ataattatgg aattgccttt aaattatata
     1681 ggcaattcct ttttatattt aaaagaatta ttaaattatt gtagataaaa gaacattatt
     1741 aatcctgttt gataaattta aaaatttatt tttgaagtag tatgatttat aaagataata
     1801 aaataaaaaa gattcattta taattataaa taaaattaaa ttatatatta taaatgttta
     1861 gtattaatat aaaaacatca atacatattt ttgtaaagaa aaaataaaga atttattttc
     1921 taaatactta ttaatta
//
LOCUS       DAEMHW010000338         1908 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.341, whole genome shotgun sequence.
ACCESSION   DAEMHW010000338 DAEMHW010000000
VERSION     DAEMHW010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.341"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            165..1076
                     /locus_tag="KPK67_003773"
     CDS             165..1076
                     /locus_tag="KPK67_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG2460661.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1251..1748
                     /locus_tag="KPK67_003774"
     CDS             1251..1748
                     /locus_tag="KPK67_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG2460662.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMGFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
BASE COUNT          796 a          140 c          347 g          625 t
ORIGIN      
        1 aataaataaa ttaagatgat aatatccaaa gtgagaaaga taatagaaag taaataaaat
       61 catgtaagaa taatttagat aagattctaa gattttattt atttaaaaat tagtatgatt
      121 aatttagatt aaataaattt aataatttta gtaggtggta atgaatgaaa ataaatagat
      181 taacagaaat aatagtaatt ctattaaata aaaaattagt aacagctaaa gagttggcag
      241 ataggtttga agtttctaca agaaccatat atagagatat agaaactctg tctatgtcgg
      301 gagtaccagt atatatgacg aaaggcaaag gaggagggat atctttaata gaagaatatt
      361 ctatagacaa ggctatattg tcaaaaaaag ataaagagag tttaatagtg gcactgaaaa
      421 cattacaggc gactaagtat ccagaaataa attcagtagt aaataaaatt ggctcaatat
      481 ttggagagca gaatttttca aactggattg aaatagactt tactgaatgg ggaagtaatt
      541 ttaatgaaga tgataaattt acaaaaataa aagaagctat tttaaggcgt aatactataa
      601 attttaatta tgtaaatagt ttgtcaagcc agacaaacag aactgtcgaa ccactgaagc
      661 ttatgtataa gagtaagact tggtacttgt atggattttg taaactaaaa gatgatttta
      721 ggatatttag aattagtagg ataaggaatt tatctataaa ggatgaagta ttttcaagaa
      781 agataataga agaagtatgt ttgaatgatt ctaaagtaat taaggaaaat actataacgt
      841 tgaaattgag gtttaaagaa aagatgcttt ttagagtatt tgatgatttt aataaggatt
      901 taataactaa aaatgaagat gatacatatg atgtaattac agaatttcct attggtgaat
      961 ggatttatgg atatatttta tcatttggtg acaatgttga agtacttgaa cctaaagatg
     1021 ttagggataa tgtaataact agactaaggg agttgtctaa aatttattca ttataatata
     1081 atgctagatt aaagatttta ttaaatatat aatatcttgc atttagtata taatatctca
     1141 ctgagtttaa ttatattaaa aatatgacat aaaggtgtcg tatattataa attataattg
     1201 aattagttca aaataaaata gattaataaa aacaaagtga ggtataaagt atgaattatg
     1261 aaatagtaga gataaatgag aaaatagtta taggaatatc gaaggagact acaaataaag
     1321 atggacaagc ggtaaatgat ataggtgaat tgtggaaaaa atttatgggc aaagggatat
     1381 ataatgctat aaagggtaaa aagaatgaca agacaatagg tctatataca gattaccagg
     1441 gtgattttac aagtccgtat agctttgttg catgttgtga agtaaatagt aattctgata
     1501 aagagaagaa tttagaagaa ttaaacacta ataatactgt tggagaaagt attattagta
     1561 aagttatacc agcaggtaag tatgcaaagt ttgttatagt aggaggtcaa aaagaagtag
     1621 gagatttttg gtttgagttt tggcaaatgg gttttgatag gacttatata agtgattttg
     1681 aagagtatca atgtaatact tttgatacaa agaaacaaga aattcatatt tacataggaa
     1741 taaagtagat ttgttattac tatattgaat gaagtattga gtatagtaca tttattatag
     1801 atagttttat tatttataac tataagcagt aaatctgtaa aagatttact gcttttttag
     1861 taattaatag tatcagtatc actttgaatc ttcagacata ttcttcat
//
LOCUS       DAEMHW010000339         1871 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.342, whole genome shotgun sequence.
ACCESSION   DAEMHW010000339 DAEMHW010000000
VERSION     DAEMHW010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.342"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(167..1558)
                     /locus_tag="KPK67_003775"
     CDS             complement(167..1558)
                     /locus_tag="KPK67_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG2460663.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
BASE COUNT          671 a          331 c          192 g          677 t
ORIGIN      
        1 tataaatctt atacactcta attatcacaa ttataatttt aattataata tctatttgtg
       61 taaagctttt tttcataatc aaagaggaaa ttacatttaa ttttatattt aaatgtaact
      121 tcctttattt aaagtaattc atttatttac aataattcgt catgaattat ttttcttttg
      181 ttagtaaatc catagccatt aatcttgtta atacatttct tattcccaat aatattaaac
      241 caaatattat taaaactgtt gctattcctc caaatatctg taaaaaacca agtgtggctg
      301 taaaaccagc aatctttcct gataataaac ttgcaaaaaa acttgttaga taccaggctc
      361 ccatagataa cgtagcatac ttagctggag ctaatttatt gaacattgca agtcctatag
      421 gagataagaa taattcacct actgttgtaa atatataagt caatactata aacaatatac
      481 tagcctttac cattccatct ggaccattag ctaatgtagc aaataccata actataaacc
      541 caacacccat tattatcatt cctaatccca ttttaactgg aatagagaaa tcacctcttt
      601 tggtatgaga taatttaacc catagggtag ctaatactgg gcataaagcc acacataata
      661 tagcatttag tgatgtaagc catgctactg gcatttcaaa tccaaataga gttctattta
      721 cattatccct tacataaatc ataaaagaac tttgtgtttg atagtacgct gtccagaata
      781 agaccacaaa taaaaataaa ataaccattg ctattatcct atttttctcc aacttagtaa
      841 gtggtccata ctcaattttt tcacctttat tacctttagt cacaggatat tttccgactt
      901 catttaataa ttttggtgca agaagtgtaa atataatcat acctaacatc atgcctatac
      961 cacaaattaa aaatgccatc ttatatccat aagatactat atctccactt gctcctctta
     1021 ctgcaaatac attatcagaa agcaatccac atattattgg agctataaat gctcctacat
     1081 ttactgccat atagaataaa ctgtatgctg catcttttct tgacacatca gttttaggat
     1141 atatatcccc aactatattt gagcaacttg atttaaaaaa accatttcct aaaataatta
     1201 ttgatagacc tacccaaacc attgcaatag atgtagatga agcaaatagt acgaaatctc
     1261 ctattgctat taaaagtgca cctatagaaa tacatttttg ttgacctaaa actttatctg
     1321 ctaaccagcc accgactaat ggtgtaagat atataagacc tgtaaataag ccaaacatgg
     1381 atgttgcttc ttgagtagaa agacctaaac caccttgagc tacacttgct gtaaaaaata
     1441 ataccaataa agctgttaaa ccataataac taaatctttc ccacaaaatt gtcatgcaaa
     1501 taagccaaaa actcttcgga tgaccacttt ttttagatac gctttctgta cttgacataa
     1561 aaaaacgccc ccttttgttt tatagttaaa tttaatatta atattgcttt aaccatttta
     1621 gcacatactt ataatatcat cttttctatg tttactcaat agattatcgc ctgtattttc
     1681 atttttttat tattattttt aaaattatga aaaatattat ttttttagta tcttcagcag
     1741 aaaatcttaa tatatatata tttaaataac aacttaaatc ttcaattctt ttttgtttat
     1801 tttttcattt ttgtaaattt ttattgaaaa aattcaaatt aaaaatattt ctttttaata
     1861 tctttttgca t
//
LOCUS       DAEMHW010000340         1739 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.343, whole genome shotgun sequence.
ACCESSION   DAEMHW010000340 DAEMHW010000000
VERSION     DAEMHW010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.343"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            619..1350
                     /locus_tag="KPK67_003776"
     CDS             619..1350
                     /locus_tag="KPK67_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460664.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNANNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EAVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(1507..1713)
                     /locus_tag="KPK67_003777"
     CDS             complement(1507..1713)
                     /locus_tag="KPK67_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460665.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT          726 a          179 c          192 g          642 t
ORIGIN      
        1 aaaggagctg tcgcatgagc aaattaaatt tgttctatga tagctctttc tttacttatt
       61 ttaattaggt atatttttaa ttattttata taaagcttat gttctaagta aaattatgca
      121 taacaaacaa aggtataaaa ataccatttt ttcagataat atttaattat aaataataaa
      181 tttagaatac ttcttttaaa tgatgagagt ctagaaattt ttatgtgtat tctttaaata
      241 aaaatttcca gactctagcc cctttttgaa tattttaaaa tttaacttaa actaagcaaa
      301 ttctataata gcttatctct aaatatttaa atttaattta tcatacaata ttacttatta
      361 taaaacttaa aacatataaa aatatatttt aaagcaataa ttttacttta cttattaaat
      421 atttggagtt attaaaatct tttctatcta aaaaatattg atattaaata tctaataaga
      481 aaatttaata agaaaaatat agattatatc aaaaaaatat tctattataa tctttagaac
      541 aaacagtagt aaatgttatc ttcataaaca aatttatttt tcacaagtac aatacttatt
      601 taaaaaagga ggtttaatat gttaagtaaa aaaaatgtaa gtttaattat tttagtaata
      661 attttaagtt tattaactgc tatatttatt ttcaataata aaaatgctaa caataaattg
      721 aaagaagtta ctaagaataa tgaagaaata gtacctaaaa acttagaagg tttaagtata
      781 gaacaattaa tagagattag agataaagcc attgataaaa atttagaagg tcttagggaa
      841 tttggagaag attcagtaaa aaaacgtaaa gattcaattt taaactttga atttggatta
      901 gtagcttttg ttaacaagaa ttatagcaac ttaacagaaa aagagaaatt aataagacaa
      961 attaatattg ttaactatga aaactctata aatgatataa tggatccttc aggttcattc
     1021 tttgaattag caaatcagaa ttaccaagtg aatgagtttc tgttaaaaaa cggaaaagat
     1081 tataaagaat ggtcatatga aggtctagat tttctaattg atcatttaga agaactgtgc
     1141 aaatttgttg attttgatgt aaaagatgta atagatatta ttgatcctac aataaaaaca
     1201 gatctttcag aggaacaaaa gaaatctttg aatgataaat taaagaaaat atcttcaagt
     1261 gatactttaa gtgaagcagt taaaaaggag ataaaaaact gggaaaacaa tttaaataat
     1321 ttaaatatga ataaaaataa ttctaattaa ttttctcata agaaaaatgc ttatatcgtg
     1381 ataatgtgaa atgtacataa ttataaacaa aaaaggggct gtgacactaa aaaatagtgc
     1441 tacgcccttt tttatttgta ataatcatca atacaaatta ttttattata tgtaattatt
     1501 tatgtcttaa ccaattttat cgaaaataac agtcttctct tttaaagaga tttttcctac
     1561 ttgatatcca tattcattta attccttttt atatttcaaa aacgaatggt ctatctctat
     1621 cccagcaata tcttttattt cttcaaaagt taatttaaac gattgactat tgtttttctt
     1681 aacatattcc cataaagaac tatatttact catcttatat cactcctttt aaaatcata
//
LOCUS       DAEMHW010000341         1719 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.344, whole genome shotgun sequence.
ACCESSION   DAEMHW010000341 DAEMHW010000000
VERSION     DAEMHW010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.344"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            62..775
                     /gene="rgbR"
                     /locus_tag="KPK67_003778"
     CDS             62..775
                     /gene="rgbR"
                     /locus_tag="KPK67_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBG2460666.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNAAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            769..>1719
                     /locus_tag="KPK67_003779"
     CDS             769..>1719
                     /locus_tag="KPK67_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG2460667.1"
                     /translation="MLNFMNELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQEKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIII"
BASE COUNT          737 a          131 c          245 g          606 t
ORIGIN      
        1 ctttaaatat attataaata ttttaattgt attaactaga aattatgtaa ggaggcattt
       61 tatgattaag atagcagttt gtgaagatga aaaagaaaca caacttttaa tagaagatta
      121 ccttgagaat atattaaaag atataagtat agaatatgag atacaaaagt atatatctgg
      181 agaagagtta ctggaaagta atttaaaaga tatagatata ttacttcttg atataaaaat
      241 ggaaaaatta aatggaatgg atactgcaag aaagattaga gaagtagaca atgaaatgga
      301 aataatattt gtaacttcat taatagatta tgtgcaagaa ggttatgagg taagagctta
      361 taggtattta ctaaagccaa tagagttgga agaactaaaa aaacatgtgc taacttgtat
      421 caaagatatt gaaataaata aggaaagtca tattacaata aaaaataagt ctaatacata
      481 taagatttat ttaaatgaaa taaaatatat agaagttcaa aaaaaagata tgctaataca
      541 cacaataaac aagaattttg atataaaata tagtttaggt aaaatagaaa aagaattaaa
      601 tccatataaa tttataagat gccataaaag ttttatagta aatttaagat atgttgaaaa
      661 tataaagcct aatgctgcaa tactggaaag tggagaagag gtacctatta gtagatatag
      721 atataaagaa gttaaggaga aatttttaaa atttcttggt gatacaatat gctaaatttt
      781 atgaatgagc tattagtagt tttatctata tttatacagt tttcaataat gaacggaatt
      841 ttaatttata aaaaggatag taaaaaaaat gaaaaaatag tattctatat tgtcttgttt
      901 cttatttata ttataattca gataagtaga ttatttaatg gaggaaatta ctttagtaat
      961 ataaactatt tattttatat attagcattt attgcttggg gaggatacta taattatttt
     1021 tataagatgg gttatattaa gtattccatt ttcttttatc tatttagtgt atattataaa
     1081 tgtttggatg ctgttatata tagaatacta ttttttgcaa ttacaggtaa tgttatttca
     1141 tctaaagaat ccttattagt acctcactta gaatctataa aaattcagat atatagtaat
     1201 ttaataatta ttcttatata tcttatattt agctttttta ggaagtctat aaaattaatt
     1261 agccaagaaa agagaaatta tgtatattta ttatttgcct tattagttaa tacaatgaat
     1321 atgcttgtaa attatgtatt aaaaaaatta gaacggtttg ggtcattaca ttcagaagga
     1381 tactattttg ataattttgt taatccaaaa ttggtaggag ctagtagtat atttcttata
     1441 ctattgttta aagaaataat aaaagagaat agattaaaat ctcaagctga attaataaaa
     1501 aataagttag atatgcaata tgcgcattat ttaagtattc aagaatctca tatgcgagtt
     1561 aagaaactat atcacgatat aaataatcac atttattgta tagacaacct tagaaacaat
     1621 agcaaagaaa ttaatgaata tgttaacaat ttaaaagatg aaataaaaac atttaaatat
     1681 atctataata caggaaatat gatattagac attataatc
//
LOCUS       DAEMHW010000342         1660 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.345, whole genome shotgun sequence.
ACCESSION   DAEMHW010000342 DAEMHW010000000
VERSION     DAEMHW010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.345"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(102..1049)
                     /locus_tag="KPK67_003780"
     CDS             complement(102..1049)
                     /locus_tag="KPK67_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG2460668.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(1018..1464)
                     /gene="lspA"
                     /locus_tag="KPK67_003781"
     CDS             complement(1018..1464)
                     /gene="lspA"
                     /locus_tag="KPK67_003781"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG2460669.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
BASE COUNT          578 a          282 c          153 g          647 t
ORIGIN      
        1 tttaaacaaa atactttaat ataagatatt ttatttaaat ttcactaaat ttatttactt
       61 atataaaaac aaaaaatgat ttataagtat tatacaaata gttattttat ctttttaata
      121 acttccttaa aatactcagg taattctgaa ttaaattcga tatattcctt agtagtagga
      181 tgtataaatc ccaacttctt agcgtgcaaa gtttgtcctt ttaatttaaa tttagtttct
      241 tttggaccat atacataatc acctaaaagt gggtgattta tagacaaagc atgaactctt
      301 atttgatgag ttctaccagt ttcaagccta gctctcatat gtgtaaactt atcaaatctt
      361 tctacaactt caaagtgagt gattgcattt tttccatctt ttactacagc catttttaat
      421 ctatccttag gatttctccc tataggttta tcaacagtta ttttatcttc tttaaccaca
      481 ccatagcata taaattcata ctccctagtt atagtatgtt cctttaactg ttctgctaga
      541 aaattatgtg cataattgtt cttagctatc ataagaagac cagaagtatc tttgtctatt
      601 ctatgaacta taccaggtct aataactcca tttattgatg acaacttatc tttacaatga
      661 tataaaatcc cgtttactaa cgtattttca tagtttccag gagcaggatg gacaaccata
      721 tcctgaggtt tatttattat taagacatca tcatcttcat atacaatatc tattggaata
      781 tcttgaggta aaacttctaa tactttaggt tctggtatct ctataacaat attatcttcc
      841 tctttaacca gatatctagc tttttctact ttattattaa cctttacttt tccatctttt
      901 ataatttttt gtatataact tcttgacata tcacctaatt gttctgctag ataaatatca
      961 agtctaactt cttcttcctc ttcaagtact aaaaactctt ttacttcatc cattatatca
     1021 cctactttta ctttcaaaaa ataaaacata tatacataaa aacacagttc ctacaactac
     1081 aaatacatca gctatattga atacatattc ccatataatt ctaaagtcga agtaatctac
     1141 tacaaaacct aatcgtactc tatcaattag atttcccaat gcaccagata taattaatat
     1201 aattcccaac cttccaaata tatgtacttt ccttgtatta agatagtata gtccaaatac
     1261 tgttgcaagt aatgcaacaa ttataaatat ccattgatta ttttgtaata atccaaatgc
     1321 tgcacctcta ttttcgacat aagttaaatg aaatacatta tttattattg gtattgttga
     1381 tacatccacc aaattattca atacccatat tttagacagt tggtctaaac ctatgagtag
     1441 aattattatt aatatatata gcaatgattt ttccccctat aaattattta aaaaccaatt
     1501 ttatttgaag cacactatta ttattataca aaaaaaatgc tctaaagagc aactttatta
     1561 aaattgtatt ttcttaataa agctagaaca aaatttaaaa tagtactact ttttttatat
     1621 tgtagatttc tacatattta aaatcatatc attatgtata
//
LOCUS       DAEMHW010000343         1656 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.346, whole genome shotgun sequence.
ACCESSION   DAEMHW010000343 DAEMHW010000000
VERSION     DAEMHW010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.346"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            159..581
                     /locus_tag="KPK67_003782"
     CDS             159..581
                     /locus_tag="KPK67_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBG2460670.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            1109..1477
                     /locus_tag="KPK67_003783"
     CDS             1109..1477
                     /locus_tag="KPK67_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2460671.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
BASE COUNT          707 a          159 c          202 g          588 t
ORIGIN      
        1 ttttaagatt ttatttttat atgatagtat acatacttat ataggtaaga ttttagctat
       61 ataaatagtt ttaacatgat ttaatatggc atattatact attttaactt aaaaattaac
      121 ttataaatat taaatttata tacagaaggg aaaacgatat gaatacattt aaaactgaag
      181 ttttattaaa atattctgca aaagacatat ttaatatatt tacaaaatct gcaagactaa
      241 attttcctaa ttttaatgag aaaaaagcag taggaacatt tgtacagcaa aaaaataata
      301 aaagatttaa ggttgaaata actaatttcg aaaaaaatag agtatatgag ataaaaacaa
      361 gcaataaaag agaaagtact attactagat atgaattagt acatattgac ttagaaaata
      421 caaaacttat ttttattgaa agtgagagca agaggaatat atttggaaaa atcaattcag
      481 ctcttgtaag agtattgttt ggaaaaaggc aaccagaaga atttaataaa tttataaaaa
      541 aactagaaat ggaattggag aataatagtg ttaatttata gatataagtt agattatata
      601 tttaaaatct atctaaaaac atatttctaa gtattttatg agtcctacta ttcgttacaa
      661 taattaatga aaaaactatt gataaacatg tgacactatg actatatacc acattaagag
      721 tatcattagc aataataatt ttatgtatta ttgcttttag ttaaaaaaag atagaaaaat
      781 taataatctc taatatccct taaactttca ataaagagga taatctttat ctaataagat
      841 tatcctcttc taatttagaa aagatattaa tttattcact atatagtatt tttgtacaaa
      901 tcaataaaaa atatatgtca tattcttatc tctaatagaa aatatattcc cctcttccat
      961 aaaactctca acatcttttt tatctataaa tctatactac ttagttctca agcataaaat
     1021 aatataaaga ttatttatac atgatgatat attatattga ctattagaca tttgtctaat
     1081 ataatcaagt taaaggtgag tgattatatt gaaaaaaata ccaaaatccg agttgttagt
     1141 tatgaatttc atatgggata aaggcataga taaaatacca gccagagaag tagctaatta
     1201 tatggaaatt tctcatggat ggacaaaagg tactactggt aaagtacttt ctagattaag
     1261 tgataaaggt tttttagaat ttgaaaaaga tggcagaaat actttataca ctattaaagt
     1321 taaacatgaa gaatatctaa aatttgaaac aaaggatttt ttagagctta tacataaaaa
     1381 ttctattcat agctttatat catcattagg ttgtggaaat gaaatatcag atgatgacat
     1441 aaaagaactt gaacaatggc taaaaaaaag aaaataggag acgtatttta tggaattatt
     1501 tatagaagtt attaaattta atatttacat aagtccacta attctttttt tcattttatt
     1561 tactaaaaag ataagcaaat atacttttaa gtttaattat tttatatgtc ttttaatagt
     1621 agtcagaatg atgtttatat ctaaaataaa tttaaa
//
LOCUS       DAEMHW010000344         1614 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.347, whole genome shotgun sequence.
ACCESSION   DAEMHW010000344 DAEMHW010000000
VERSION     DAEMHW010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.347"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    505..768
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            919..1470
                     /locus_tag="KPK67_003784"
     CDS             919..1470
                     /locus_tag="KPK67_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBG2460672.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          655 a          154 c          246 g          559 t
ORIGIN      
        1 gtcatatttt tggttatagt tttagtagtg ctaatattta ttaaaagaaa gaagaaaaaa
       61 ttttactaaa aataattaaa agaatatcat ttttttaatc attggtatct atttaataat
      121 atgatgtaat taaaatggag taatattctg tttcaaagga ggcatataaa tacaggaatg
      181 agtattaaat gatggttaat taagtaaatt tattgataaa tgtacttaat taactttgaa
      241 gaatttaatt cctcatgaat caaaatataa aattacatca acagttattc acaaaattaa
      301 tataagaaga attagagcca aagagcagac tattagacca ttaaagtaat tattcataga
      361 attttgtata tgaaaagaaa tgtatagtat aatttacttt ttttataaaa aatagattac
      421 aaaatatatt ttttataaaa aggtattgac attttttaga aaaaagaata tcattatttt
      481 aacaacaaaa ttcaatacaa taaattaaat cctatgacaa ggaagagtag ttaaatttag
      541 gttcagtata gagagctgag gatggtggaa tcttagcaag aaaaaatttg atgaatggcc
      601 cttagaggaa taaattgaaa tcatagtttt gagagtagac tttatcgtca gtcgcacgtt
      661 atagcgaata aggtatgtaa gtacctgata atgagatgta tgaaaattga attttatatt
      721 aatttttata taatttaggt ggcaacgcgg ataatctccg tcctattagt ttaggacgga
      781 gttttttatt ttactgaaaa tttaaaagat agaaggtggt gagaaagatg ttatgaattt
      841 caaaagctaa agatgatgat atagtaggtt ggcaaaaaaa taaattgaaa attaaaaaaa
      901 taggaggaag tacaaaaaat gaaaacaaat acaaaaaaat taactttaaa tgcaatactt
      961 ttagcgatgg gattattaat acatcaaatc acaccagcta taggattacc aatgaagcca
     1021 gatgtaccac tagcaatgtt atttgtaata ttggttttaa atagagatga ttataaaact
     1081 tgtttgatag cgggtatagt aacaggaata tttacagcac ttacatcaag ttttccggga
     1141 ggtcaaattc ctaatgtaat agataaaact cttacagcaa atatagtatt tttattaatg
     1201 aatattagtt ataatatgcc ttttataaaa aaattagcta agaaaacaca agatactgta
     1261 gtagttgcaa taataatgcc aataggaact cttgtaagtg gaactatctt tttattagca
     1321 gctcaagcta tagttggtct tccaggagct tcatttacag ctttattctt agcagtagta
     1381 ttaccagctg ttttaataaa tctagttgca ggaatatttt tattcaagat tgttagtcta
     1441 tcaataagaa gagttagtta tcaagcttaa acacgaaata atattttact ttataaagca
     1501 ttataataac taaataacat aagaataaag tagattataa ctattttatt cttatgttgt
     1561 ttaaagattt ggtattatat atttattttt aataaaatat ttatatagta ccaa
//
LOCUS       DAEMHW010000345         1571 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.348, whole genome shotgun sequence.
ACCESSION   DAEMHW010000345 DAEMHW010000000
VERSION     DAEMHW010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.348"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            53..1291
                     /locus_tag="KPK67_003785"
     CDS             53..1291
                     /locus_tag="KPK67_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG2460673.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT          671 a          139 c          267 g          494 t
ORIGIN      
        1 aaaatactta tctagttact agcaattatt agtagaaatg ggggaaatag agatgaataa
       61 gggattttac aaattaaatt cagtggtatt gaactttgat attaagaatg atgttaacaa
      121 gataagattt aatcattata taatgagaaa agaaaacata caaagacttc aaggaactct
      181 gccaaacgga caattttata tgacagtgag aagagctgct acagatttac aactttctat
      241 ttctactata agtaggcttg taaaagagtt tttggattta gggataataa gattagtgtc
      301 taagggtgta aaaggtaatc gttctgtata tagttatgta agtatagagt ttaatggaag
      361 tttatttgat gatagcaaat atatggaact tgactgtgta tgtaatgatg aatttattga
      421 taatagtggc atagagtttg attgtgatag caaagataat tcttttaatg gagatataaa
      481 tgtagaaact gtgcattgca ttaatgagta tttgtctgat gatgatgtag agcataatta
      541 taacaatttc cataacaaag atactataaa ggataaagcc atttctaata ttagcactat
      601 caaaaaatat attgatgaat cttttgatac aaataaatca gaaaattcta acttacaaca
      661 ggagtcaata aatttcaact ttaatgatac atgcaatgaa atagataaaa gaacgaataa
      721 tagtatagaa aataatcaaa ataaaaggcc tatgagattg agtgacatca gtgttttaag
      781 tgataaaggt atgacaaata aaaagaatta ctttaatcat aatcatggta tacaaaaagg
      841 gactaaatct gtaacaaaga aaaaagaatc cttaaataaa tttaataaaa aaaatataca
      901 gcaaagcatt ataaatatgc taaataaaaa atcaggtaag aattttaaaa tcgattctcc
      961 aataagcata aaacttatca atgaaaggct aaaggaaggc tatgtcttag aagatttcta
     1021 caaagttatt gaggtaaaag tggctaagtg gaaaaatacc ataatggaaa tgtatataag
     1081 accagaaact ttgtttagct ataagtttga atcctatgta aatgaaaaca tatctatgaa
     1141 aagtcaaagt aatgttcacc catgtaaaga aggcaatagt aatgatagca gtaattatat
     1201 acattctgat tatacaacat atgatggcag tgtgcgtgag agaaaaaaat atgagaatgt
     1261 aagatactgg aatcctatat gtgaaattta attagtttaa gaatgtattt atgataaata
     1321 agatattaat agagaatagt gagctatagc aaatgatata tcaataatta gtagtaagtt
     1381 atattaagta agatattact atcaggtagt gaattatatt gaacgatata tcaataattg
     1441 gtagtaaatt atattaagta agatattact atcaggtagt gaattatatt gaacgatata
     1501 ttaataattg gcagtaaatt atattaaata agatattaat atcgagtaat gaattatatt
     1561 gaacgatata t
//
LOCUS       DAEMHW010000346         1494 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.349, whole genome shotgun sequence.
ACCESSION   DAEMHW010000346 DAEMHW010000000
VERSION     DAEMHW010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.349"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(78..644)
                     /locus_tag="KPK67_003786"
     CDS             complement(78..644)
                     /locus_tag="KPK67_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG2460674.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKSTSSSY"
     gene            810..1331
                     /locus_tag="KPK67_003787"
     CDS             810..1331
                     /locus_tag="KPK67_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG2460675.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
BASE COUNT          634 a          176 c          231 g          453 t
ORIGIN      
        1 ttattaataa aaatgttata taaaaatgag tatatagcat tttaaagagt aataaatctt
       61 acataataaa tgatttatta atatgaacta gaagtacttt tactatattg ttttataaaa
      121 cctttaggca ttttacttat aaaatatacc accataatac aagacaaagg tttgtctact
      181 atattactaa gaatccttgg aaagaacgca gcagtaaata tcttctgacc agactgaact
      241 aaaaaaccaa ccaataaatc agcaccactt ccacttaaac caccaaacaa taaaactgaa
      301 ataggagtac caattaaagg acaaacaata gataaaatta ctcctgtaat aatagcttct
      361 ttataaccaa aacctttttt cttagaaata tatccaacaa taagaccaag aacaatattt
      421 acaagggcaa aaggtatatt tgtaggacct gatataacac ctaaaactaa gtttgtacac
      481 ccaccaataa gagcacccca aaaaggacct aatacgacag ctccaagtac agtaccaact
      541 gtatctaaaa ataataatgg tatatttatc atagatataa ccatacctaa gacaatatta
      601 ataactatgc acattgaaga aaatgtgact tttttagtat ccaattataa tacctcctaa
      661 aaaaatatat acatttatca tagtatagat tataaaagat tactaattgt atatttctat
      721 atagaatatt ggaaatatag aacaaataaa taatataatt atcaaaatat gtagtatcat
      781 taaaattaag aataactaag gagattatta tgaagagaaa tagagagatt gtagtagtat
      841 gtcattgtat attaaattgt aattctaaag tagagggatt gtcagaattt aagggagcac
      901 aaaatatagc gatagattta ataaataaag ggtatggaat aattcaacta ccatgtccag
      961 aaatgattat gtatggaata aataggtggg gacatgtaaa agaacagttt gacaatttgt
     1021 tttttagaga acaatgtagg caaatgttaa tacctcatgt aaggcagttt gatgaatatt
     1081 taaaaaatga ttatgatata aaaagtatca ttgcaataga tggcagtcca agttgtggtt
     1141 ataataagac atgtagttct aaacgttggt ttggtgagtt aagtggttgc aatgaattga
     1201 tggataaaat aaatgatata caaatggtgg atgggaaagg tatttttata gaagagctag
     1261 aaaagctact taaagaatat aatttaaata tacctatagt tggatttgac gagcttagag
     1321 aagataagta gagatataaa tatttattgt ctcattatga aacaccatta tatttggcag
     1381 aaaaatatga ctgatggaca gtacaggaac catttttaag tggagtaatt agtacttata
     1441 aagatgtatc cactagttga aagaaaatgt agagaggtaa taaatgaatt aaga
//
LOCUS       DAEMHW010000347         1441 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.350, whole genome shotgun sequence.
ACCESSION   DAEMHW010000347 DAEMHW010000000
VERSION     DAEMHW010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.350"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            721..1377
                     /locus_tag="KPK67_003788"
     CDS             721..1377
                     /locus_tag="KPK67_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460676.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
BASE COUNT          576 a           98 c          300 g          467 t
ORIGIN      
        1 attgattaat tttattatta tttttaaggg gggagagtat ttgtagttat aaagcatatt
       61 gggaataatg aaaaaggtta aaaacatatt actataaagt atagggggta ggttaaaaga
      121 gtaatcataa aataaatata cagataatag tagataagaa taccagaaaa aaatagatag
      181 ttaaagggtt agggtaaaag agtaattgta aaataaatat atggatgatg gtggataaga
      241 atatcagaaa aaatatatag ttaaagggtt agagtgaaag agtaatcgta aaataaatat
      301 atggatgatg tggataagaa tagtaagaaa aaatagatag ttaaagggtt agggtgaaag
      361 agtaatcgta aaataaatat atagataatg gtagataaga atatcagaaa aaatagataa
      421 ttaaagggtt agggtgaaag agtaatcgta aaataaatat atggatgatg gtagataaga
      481 atatcagaaa aaaatagata attaaagggt tagggtgaaa gagtaatcgt aaaataaata
      541 tatagataat ggtggataga gtagtaagaa aataaataga taaagggata gaataaattt
      601 aaatatggaa aatgaatatc taataataag tgattaataa ataattgtag atagattgta
      661 aattaaataa atctttaata gaaaggagaa ctatttatat attaaatagt atttataaat
      721 atgaaaaaag gcgattttat atggagtgga gttcttattt gtattattgc tgtcttagca
      781 ataccaagta gtagagaagt atttatggaa gcaacaggtg cacatccata tattggaggt
      841 tttttaaagt ttgctgtatt agctacaatg ggagatttac ttggtgctag aattttgtat
      901 aaggattgga aaataccagt aggagtattt tatagagcaa tagtttgggg aataataggt
      961 gtagttacca catatggaat gtcaatctac tcatcaggtg taggtcaagc acaagtggat
     1021 ggtatgcttc catttgaggg aatagctttt gcaaatgctc tatttaagag tgcaactatg
     1081 aatatactaa tgtcaccaac tgtattttta ttccataagg ttatggactg ttttatagat
     1141 aaaaaatatg aggttggaaa ggggattaag gtaaaactta aagatgtggt aaatattgtg
     1201 gactggaatg cttaccttgg ttttagtgtt ttaaaaacta taccattttt ctggatacca
     1261 tgtcatacaa ttgtattctt attccctgct gagtatagag taattgcttc tgcatttgca
     1321 tctatagctt taggtctaat attggcttta gctaataagt ctaaagttgc agtttaggtt
     1381 cactgatgaa gatagtagta ttatttaaat taggaataac tttttaaatt aatattattt
     1441 g
//
LOCUS       DAEMHW010000348         1439 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.351, whole genome shotgun sequence.
ACCESSION   DAEMHW010000348 DAEMHW010000000
VERSION     DAEMHW010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.351"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            218..595
                     /locus_tag="KPK67_003789"
     CDS             218..595
                     /locus_tag="KPK67_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG2460677.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            complement(1032..1295)
                     /locus_tag="KPK67_003790"
     CDS             complement(1032..1295)
                     /locus_tag="KPK67_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460678.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
BASE COUNT          548 a          116 c          184 g          591 t
ORIGIN      
        1 tatgtcaaat attagaattt taatttgata atctgttata cttatagaat agatatttat
       61 ctttatatta atagattgtg tgtttaattt tgaataaaaa tataaatttt ttgtattaat
      121 aaatttggat gagatatttt aaataaaacc tttgactcga aaagtctatt aatatagtat
      181 ttaattatgt agaagtttga gaggagtgta aaatcatatg actattacta agataccacc
      241 agcagaatta aaagtcatga aatttatttg gagttcagat tttacagtag catcaaaaga
      301 tgtcattgaa gctatggaaa aactatataa ttggaagcaa actacaacac taacactttt
      361 atcaagatta gtaaataagc aattcttaag tgcaagaaaa attgatagac atacacatta
      421 tacagttctt attggagaag aagagtattt acattttgaa acaaaaactt ttttagataa
      481 tatgcatgga agttctattg agagttttat gaaatccttg ttcaaaaagg gggtaaatga
      541 agaagaattg aactctgttg aaaaatgggt taaagatatt aaaaaagatg aataatttgg
      601 tgtagttaat tatcataaaa taaatagttt ttacaataat tagttaaatg aatcaaatat
      661 aaagttttat gtaaaatttg tgatttttat aaatattttt gtcttgttat ttaatattaa
      721 tacaataagt ttgatgtaaa atcaaacctg tttgaattta atttatattg ggtttgtcat
      781 taatattatt ttagtttgat attgtatacg aatttaaaag tcagaatttt tgtatattaa
      841 tcatgtaaat taattgtttt agatataaag ggcataattt tatttttgtt ttgattattt
      901 ctagggtaag ataagtatac aatagattgt attaaacatg cttatattga tagatttgag
      961 ataaatattg agatatatta ataattatta atataaaaaa gttcctagtt tttaggaact
     1021 tttttatttg attaatacag agtatgagtt tttgcttcag ctatagctct tgctgcactt
     1081 ttaggttttt cagtattttc ttttttgtaa aatttaatca cttcttctaa atctttattt
     1141 tcaatattat gttttttgtt ttcaagaata taatttacaa ttgattcatt tatcaaatct
     1201 gtcaaaatat tattttcgtc attagttatc ttagacattt cttgtaataa aatttcatta
     1261 acttctactg taatttgttt tgtatttttt atcatatcaa ccacctctta agaaaattat
     1321 atcatctcaa aaaaataaat tcaatatgtt ttaaaattat agaattttaa ttcttaaatt
     1381 ctaaaaaaga gaaaattaga taaagtagtt ttagatttta aaagattaat aaaaaatat
//
LOCUS       DAEMHW010000349         1437 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.352, whole genome shotgun sequence.
ACCESSION   DAEMHW010000349 DAEMHW010000000
VERSION     DAEMHW010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.352"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1218
                     /locus_tag="KPK67_003791"
     CDS             <1..1218
                     /locus_tag="KPK67_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG2460679.1"
                     /translation="VLLSKKEILDKVVVFNKKYINVNKKNIYNRDEKMGTYYSLQEIT
                     YIKKLEQNLTKKLREKGQIAKYTFKDIKTNSPKMFECIDLAKKVSKSDLSILIRGESG
                     TGKELIAQSIHNNSNRKNQPFIAVNCAAVPENLLESQLFGYDKGTFTGGLKDGKQGLF
                     ELANNGTIFLDEIGDMPLELQTKLLRVLQEKQIMPVGSHNVINIDVRIISATNKNLEQ
                     MIDNSQFREDLYYRLNTIPINIPPLRERKEDILIIMEDLINKKLVITPEAKKLIQNYM
                     WKGNIRELQNVTSYLNIMCEDIVLEKDLPPNLRSSDNKNTSLKLKYSKNDILNILEIL
                     ILNKESDVGIGRGLILKALLDKNLQITEGKIKKIFEYLKKEELIICSSGRYGSKITQK
                     GEDFYNKLKYKGL"
BASE COUNT          640 a          156 c          204 g          437 t
ORIGIN      
        1 gttttattga gtaagaaaga gatactagat aaagttgtcg tattcaataa gaaatatata
       61 aatgtaaata aaaaaaacat atacaataga gatgaaaaaa tgggcacata ttatagttta
      121 caagaaataa catacataaa aaaattagaa caaaatctca ctaaaaaatt aagagaaaaa
      181 ggacaaatag caaaatatac atttaaggat ataaaaacaa atagtcctaa aatgtttgaa
      241 tgtatagatt tagctaagaa ggtttctaaa tctgacttaa gcatacttat cagaggtgaa
      301 agtggaactg gtaaagaact catagctcag tctatacata acaactcaaa tagaaaaaat
      361 caacccttta tagcagtaaa ttgtgcagcc gttcctgaaa atttattaga aagccagcta
      421 ttcggatatg acaagggaac ttttactgga ggtctaaaag atggaaaaca aggattattt
      481 gaacttgcta ataatggaac aatattctta gatgaaatag gcgatatgcc tttagaatta
      541 caaacaaaac tacttagagt tcttcaagag aagcagataa tgcctgttgg gtctcacaat
      601 gtaattaata tagatgttag aatcatatct gctacaaata agaatttaga acaaatgatt
      661 gataatagcc aatttagaga ggatttatat tatcgattaa atacaattcc aataaatata
      721 cctcctctaa gagaaagaaa agaagacata ttaattatta tggaagattt aattaataag
      781 aaattagtaa tcacaccaga agcaaaaaaa cttatacaaa actatatgtg gaaaggtaat
      841 ataagagaac tccaaaatgt tacatcctat ttaaacatta tgtgtgaaga tattgtatta
      901 gagaaagatt tacctccaaa cttaaggtct tcagataaca aaaatacatc attaaaacta
      961 aaatatagta agaatgatat attgaatata ttggagatac taattttaaa taaggaatca
     1021 gatgttggta ttggtagggg attaatttta aaagcactac tcgataagaa ccttcaaatt
     1081 acagaaggaa aaattaaaaa aatatttgaa tatctaaaaa aagaagaact tataatttgt
     1141 agttcaggaa gatatggaag taaaataact caaaaaggag aagattttta caataaatta
     1201 aaatataaag gtctataaaa ttataattta tcaaacaaag aaatctattc aggattaaag
     1261 tactccttca ataggttttt ttatttttca agtaccctaa aattataatt tattttgtac
     1321 ataaataata ggaattaata ggaataggat aattttttta tttcctattt tttttatata
     1381 aactagcata taaaatatat tttcttcaaa ataatatttt ttactgaaaa ttgaaac
//
LOCUS       DAEMHW010000350         1386 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.353, whole genome shotgun sequence.
ACCESSION   DAEMHW010000350 DAEMHW010000000
VERSION     DAEMHW010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.353"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..270
                     /locus_tag="KPK67_003792"
     CDS             <1..270
                     /locus_tag="KPK67_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG2460680.1"
                     /translation="RLISDVSWSEFVRQLEYKANWYGRQIVKVGKFFASSQICNKCGY
                     KNEEVKNLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            446..1201
                     /locus_tag="KPK67_003793"
     CDS             446..1201
                     /locus_tag="KPK67_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HBG2460681.1"
                     /translation="MAHICFEDICYLLLKEAMKLKVIPHNKIVLFEDDLSIGNISDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
BASE COUNT          577 a          110 c          248 g          451 t
ORIGIN      
        1 cgcttaattt ctgatgtgtc atggtcagaa tttgttcgtc aattagaata taaggctaat
       61 tggtatggta gacaaattgt aaaggtaggt aaattctttg caagttcaca aatatgcaat
      121 aaatgtgggt ataaaaacga ggaagttaag aacttaaata taagagaatg gatttgtcct
      181 agttgtaatg aaactcacga tagagatata aatgcaagta taaatatatt aaaagaagga
      241 ttaagactaa taacgattca aaataaataa cttatgtaag aaccgtagga actacgggga
      301 tggcctgtgg agaattagta agacatattt tagtatgcaa aattctatga agcaggaacc
      361 ctgtgacttt agtcatggga ggttcagaat tacagtttat atattataat aaaataaatt
      421 gttaatttta aaagaggtga attttatggc acatatatgc tttgaggata tttgttattt
      481 gttattaaaa gaagcaatga agttaaaggt aataccacat aataaaattg tattatttga
      541 agatgattta tcaataggta atatttctga tgtaaataac tacgaattaa gagaaaatat
      601 ttttaagatt agaaatgttt ttgaaaataa tccgaaacta ttattcaagc cagatttgtt
      661 agaagatagg aaattatcaa aatgtagaaa tataataata tggtgtgcaa ataacccaaa
      721 agatttgtgt ggagtatatt atgtagtgag taagtttcaa gataaaaata taaagattgc
      781 attagtagat gaaaaaaaga cagaagatgc tctaattaga tattcattta ccagtgaaat
      841 gtctccagaa gattttccat tctttttaga taagacacta gaaattggct ttaatgaaaa
      901 actagaattt aaaaataaat gggatgtatt agttaaagaa aattctattt ttagagcatt
      961 agtaggtaac gaaattaaaa gtgttgaaga aacttatttt gataaatata tattaagaaa
     1021 tatcagtgac aaatggcaac ctttaattga agtggttgca aactgtatgt ttgaagatga
     1081 aatgcgtgta aaagattggt ttatactttg gagattagag aagatggcaa gtttaaaaat
     1141 agtaaaaata aaaggaagta aaggggattt ttattctgat aaggagataa gaaaagttta
     1201 aaaattgaat ttataataaa ttgtattaaa taatgggtat aactatgaag aaaaatatct
     1261 taacagtatt aatattataa ttagcaataa gtataatatt tattctataa tttgaaaata
     1321 taataactaa taccttacga ataagtgtgc taaataaaaa agaaatactt atttcaggga
     1381 tattag
//
LOCUS       DAEMHW010000351         1375 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.354, whole genome shotgun sequence.
ACCESSION   DAEMHW010000351 DAEMHW010000000
VERSION     DAEMHW010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.354"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            3..602
                     /locus_tag="KPK67_003794"
     CDS             3..602
                     /locus_tag="KPK67_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBG2460682.1"
                     /translation="MKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEVRSKN
                     LKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVKFIIH
                     PYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGSIPNQ
                     KDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            900..1238
                     /locus_tag="KPK67_003795"
     CDS             900..1238
                     /locus_tag="KPK67_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG2460683.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
BASE COUNT          542 a          153 c          180 g          500 t
ORIGIN      
        1 atattaaaaa taaattcaaa aattacaaac catatataaa tggatacaag aatatgaaga
       61 gagcatctgt tttaattcca atagtagaaa taaataacac acactatata ttatttgaag
      121 ttcgctcaaa aaatttgaag catcaaccta gcgaaatttc ttttccaggt ggtaaaatag
      181 aatcaggtga gactccatat gaagctgtta ttagagaaac ttgtgaggaa cttggaactt
      241 ttagtgacaa tatagaagta atatcaagtc ttgacctcct tataacacca gtaaagttta
      301 tcatacatcc atacttagga tatataaaaa atattaataa tttaaatata aacaaggatg
      361 aagttgacca tacttttttg gttcctataa aatatctttt agagaatcct ccaaatactt
      421 atagtaatac tgtaaaagtt tttccagatg aaaatctacc atatggttca attccaaacc
      481 aaaaagatta taaatttgaa agtggaagtt atcctattct gttttataaa tataaagatt
      541 atgtaatttg gggaatgaca gcaaaaatac tagaaaactt tttaaatttt ttatcagaat
      601 aacactcata ataaattatc aaaactttta cataagaaat ttataaaagt ttctaaattt
      661 aacaattcta aaatatcaat aactatattt tgtagcactt ttttatttta ttattgtttg
      721 aatatttgat ggttttcaat gcaatttcaa cataaatact atgtattagt gtatactgtt
      781 gatgaattaa agtatgataa tctatttaat aaagtatgaa gtaaatgatt ttaatgtatc
      841 tttatggtaa aattactaat aagtaataaa attaattaaa atatatggag gtgcttataa
      901 tgtttgctgt aggatttgtt gttataattg cacttgcctt tttcatttta agtgtactat
      961 tatttaatgg aaaagccagt atgctactag caggttataa ctctatgagt aagaaagaaa
     1021 aagcacgata cgataaaaaa aaattatgta gaagttctag cattgttact tttattgtct
     1081 ctataatgct atttattatg gcgtttttgg gatatagaat agagagtggt caaatggacg
     1141 aaaaaaaaat ggttcccttt tctataatat ttataatagt catactttcc agtgtatttt
     1201 ttaatatcct ctactctaac aaaaaatgta aaaaataaat agttgtaagt tgagcagtta
     1261 attataaaag attgactgct ttttcatgta tattgactac tcttattaaa tattttaaca
     1321 tttaaaaatc tatgtaaaaa taatgaatat agtacaaact tctaataatg atttt
//
LOCUS       DAEMHW010000352         1368 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.355, whole genome shotgun sequence.
ACCESSION   DAEMHW010000352 DAEMHW010000000
VERSION     DAEMHW010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.355"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            160..1032
                     /locus_tag="KPK67_003796"
     CDS             160..1032
                     /locus_tag="KPK67_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG2460684.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
BASE COUNT          544 a          153 c          217 g          454 t
ORIGIN      
        1 aatactatgt gaagtataag gtacagaaaa gttagaattt tatgagttta agaagctatg
       61 ataaaaacta agatgaaatt gagataaaaa ataaaatttt tagagaaaaa gacagtgtta
      121 ataaaacaaa atagtttata taactaaaag gagaaaaaaa tggaagaaac aaatgaaatg
      181 aaggacagta aacatttgat atcaaatata caatctcaat atacgagatt gagtaagggt
      241 caaaaactta tagcacaata cattttaaat aattatgata aagttgcatt tatgactgca
      301 tgtaaattgg gtgaaaccgt aggagtaagt gagtctacag ttgtaagatt tgcaaatgca
      361 ttaggttata gtggatatcc taaattacaa gctgcacttc aagaactcat aaaaaataaa
      421 ctaactacag tacaaagagt tgaaatggca catgattatt ccgatgattt tgcaatacta
      481 aacaaagttt taaaaagtga tatagataat ataaggtcta cactagaaga aattgacgag
      541 agagcgttta aagaagcttc taacaaactt ttaagagcga gaaaaatata tatacttgga
      601 atgaggagtt cttttgtagt agctcaatat ttaggttttt acttagatat aatacttgac
      661 aatgttcata taattagaat ggatatggga gatgcttttg agcagattgt aagaataaat
      721 gaagaagatg taatagttgc tattagtttc cctcgttatt ctaagaaatc ttatcaaatt
      781 gtcaattatg ctaaggaaaa aggagcccat gtaatatcat taacagatag cttatttgcg
      841 ccagtagcat cacttgcaga taacactctt ctagttaaga gtaatatggc ttcatttgta
      901 gattctctag ttccagctct tagtatttca aatgcccttg ctatatctgt tgggatgaag
      961 gaaaaagaag atattaaaca gcattttgat gatttagagc aaatttggaa aagatattct
     1021 gtgtatgagt aaagtttgta tattaataag gctatgaaat tgagaatcat agccttatta
     1081 gtaaatactc gattatcact taaaaaataa attttcgttt aaataataca aataaatatt
     1141 tcgagactca aaacataaag atgtattctg aatacatctt tactacgtca actaatttat
     1201 taaagaaagc atttccattc atggagagtg ctttttttat ttctctaaac aatatcattt
     1261 ccttaattaa caattataaa attaatttta tttgtattta agtatataat atcttattaa
     1321 aattctatta aaatatgtat ttctatcttt tttgttcact ataatatg
//
LOCUS       DAEMHW010000353         1341 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.356, whole genome shotgun sequence.
ACCESSION   DAEMHW010000353 DAEMHW010000000
VERSION     DAEMHW010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.356"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            135..1232
                     /gene="ychF"
                     /locus_tag="KPK67_003797"
     CDS             135..1232
                     /gene="ychF"
                     /locus_tag="KPK67_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBG2460685.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT          530 a          145 c          242 g          424 t
ORIGIN      
        1 agtttacaag cttgatatat atgatgtata atcaatatgt atgtctataa ttaaaacaat
       61 tttgcaatcc tatagattga ttgtttaaag ttatataaag aatataaaaa agaataagag
      121 agaggacgat taatatgaaa ttaggtatag ttggtttacc aaatgtaggt aaaagcacac
      181 tatttaacgc aataactcaa gcaggcgcag aatctgcaaa ttaccctttt tgtacaatag
      241 acccaaatgt gggagttgta tctgttccag atgaaagatt aaacaaacta caagaattat
      301 ataattctga aaaaatagtt cctactgcta tagaattttg tgatatagca ggtttagtta
      361 gaggagcttc taaaggagaa ggtttaggaa ataaattctt atcacatata agagaagttg
      421 atgcaatagt tcatgttgtt agatgttttg aggatgaaaa tgttgtacat gtagatggtt
      481 ctgtagaccc acttcgtgat atagaaacta taaacttaga attaattttc tcagatatag
      541 agattcttga aagaagaatt aataaaactc aaaaagctgc taaggctgat aaaactttag
      601 gttctgaact tgatttatta aaatcaataa tgtctacttt agaagaaagt aagtgtgtaa
      661 gaactatgga atttacagaa gatgaacaaa cattcgtaaa ttcattggat ttacttacat
      721 ctaagcctgt aatatatgct tcaaatgtat cagaagatga tttagctgat aatggagaaa
      781 ataataaata tgttcaacaa gttaaagcat ttgcagaaac agaagatgcc gaagttgttg
      841 tagtatgtgc acaaatagaa gcagaaattt ctgaacttga ttctgctgaa gaaaagaaag
      901 agtttttaga aacattaggt cttgaacaat cagggcttga taagcttata aaatcttctt
      961 atgctttact tggtttaatt tcattcttaa ctgctggtcc aaaagaagtt agagcatgga
     1021 ctataaaagt tggaagtaaa gctcctcagg caggaggaaa aatacattct gatatagaaa
     1081 gaggatttat cagagcagaa acaatagcct ttaatgattt ggttgagcat ggtactatgg
     1141 ctgctgctaa agaaaaaggg ttggttaaat tagaaggtaa agaatacata gttaaagatg
     1201 gagatgtaat attatttaga tttaatgttt aaaatgatta atcgtataca ctttgactat
     1261 tatatataat aactaaaaaa ctaagattat aataatacaa tattatctta taataattat
     1321 atagaattta aaaataaaag a
//
LOCUS       DAEMHW010000354         1337 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.357, whole genome shotgun sequence.
ACCESSION   DAEMHW010000354 DAEMHW010000000
VERSION     DAEMHW010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.357"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            27..101
                     /locus_tag="KPK67_003798"
     tRNA            27..101
                     /locus_tag="KPK67_003798"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KPK67_003799"
     tRNA            116..200
                     /locus_tag="KPK67_003799"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="KPK67_003800"
     tRNA            210..293
                     /locus_tag="KPK67_003800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="KPK67_003801"
     tRNA            323..399
                     /locus_tag="KPK67_003801"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="KPK67_003802"
     tRNA            407..482
                     /locus_tag="KPK67_003802"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="KPK67_003803"
     tRNA            571..659
                     /locus_tag="KPK67_003803"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="KPK67_003804"
     tRNA            663..738
                     /locus_tag="KPK67_003804"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="KPK67_003805"
     tRNA            745..818
                     /locus_tag="KPK67_003805"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="KPK67_003806"
     tRNA            833..909
                     /locus_tag="KPK67_003806"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="KPK67_003807"
     tRNA            933..1009
                     /locus_tag="KPK67_003807"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="KPK67_003808"
     tRNA            1017..1093
                     /locus_tag="KPK67_003808"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="KPK67_003809"
     tRNA            1102..1177
                     /locus_tag="KPK67_003809"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="KPK67_003810"
     tRNA            1185..1258
                     /locus_tag="KPK67_003810"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="KPK67_003811"
     tRNA            1265..1336
                     /locus_tag="KPK67_003811"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          322 a          308 c          355 g          352 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgag cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatataaatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DAEMHW010000355         1308 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.358, whole genome shotgun sequence.
ACCESSION   DAEMHW010000355 DAEMHW010000000
VERSION     DAEMHW010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.358"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(192..404)
                     /locus_tag="KPK67_003812"
     CDS             complement(192..404)
                     /locus_tag="KPK67_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2460686.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(405..821)
                     /locus_tag="KPK67_003813"
     CDS             complement(405..821)
                     /locus_tag="KPK67_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460687.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
BASE COUNT          507 a          197 c          116 g          488 t
ORIGIN      
        1 taacataatc ttataaatat taaaatttat ctacctataa aatttctcca ttaaatttta
       61 ttacatttta tcttaaaaca attataatct gaccttaatc acatgttata ttatataaat
      121 atttttaaca aacttaataa ttaaataact atattttatt atccatctta taaatgttta
      181 taaattttcc actattcttc ttcaataaat tcaaacaaat tttctactgt agtatcgaat
      241 actttagcga tatccatagc taacttcaaa gaaggattat atttcccatt ttctaagtgt
      301 acaatagtct cacgtcttac cccaaccaat ttagcaagtt cattttgcgt cataaggagt
      361 ttttctctgt attctttaat ttttgttttc atcaatattg ccacttaatt acatatcccc
      421 cttttatcaa agtatatata caacactaat ttagataaag actgtataaa taaaactgca
      481 atgaggatta tacctgtatc taggtttgat atatttatat tacttgtata aggtgttgca
      541 aagaaaatag atattattac tatagaatat atcattattt ttattgcaat cttattaacc
      601 tttgatagta taaattcaga tgattcatca actaaatcat tatatttttt attataaatt
      661 gaataacatc caaaaactat taaccaactc aaccctggaa gagaagcagt gcttggacct
      721 ttaaaaatat acttagtggc taatacagct ataaaaaaat aagttattac aagcaactcc
      781 aggataagct ttgatcttac accagtttct gatttagaca tataaaatca ctccttaaaa
      841 attatattct ctatactctt taattttcgt ttttatcaat attatcactt agttgcatat
      901 tccctttcta tcaaaatgta tatacaaaat taatttagat aaagattgta taaataaaat
      961 agctaagaga attacaccta tatccaaatt tgatatatcc aaacctcttg tataatttgc
     1021 taaaaagaaa acagatatta gcaaaataga atataacatt atttttgatg caatatgatc
     1081 aacctttaat aacataagtt ttgacgcctc gtcaactaaa tcattatatt ttttattata
     1141 catgaagtca catgcccaaa aagcgaataa aattataaaa gtaatacaaa gatttccctc
     1201 tttaaaaata aactttttta ataatgagtc tataaaaaaa taggatatca taagtaactc
     1261 tgaaacaaga tttgttttaa caccaagctc tgatttagac atataaaa
//
LOCUS       DAEMHW010000356         1234 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.359, whole genome shotgun sequence.
ACCESSION   DAEMHW010000356 DAEMHW010000000
VERSION     DAEMHW010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.359"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            108..224
                     /gene="rrf"
                     /locus_tag="KPK67_003814"
     rRNA            108..224
                     /gene="rrf"
                     /locus_tag="KPK67_003814"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            232..306
                     /locus_tag="KPK67_003815"
     tRNA            232..306
                     /locus_tag="KPK67_003815"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:264..266,aa:Asn,seq:gtt)
     gene            312..397
                     /locus_tag="KPK67_003816"
     tRNA            312..397
                     /locus_tag="KPK67_003816"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:346..348,aa:Leu,seq:taa)
     gene            413..488
                     /locus_tag="KPK67_003817"
     tRNA            413..488
                     /locus_tag="KPK67_003817"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:446..448,aa:Met,seq:cat)
     gene            496..570
                     /locus_tag="KPK67_003818"
     tRNA            496..570
                     /locus_tag="KPK67_003818"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:529..531,aa:Glu,seq:ttc)
     gene            585..658
                     /locus_tag="KPK67_003819"
     tRNA            585..658
                     /locus_tag="KPK67_003819"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:617..619,aa:Gly,seq:tcc)
     gene            664..739
                     /locus_tag="KPK67_003820"
     tRNA            664..739
                     /locus_tag="KPK67_003820"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:697..699,aa:Val,seq:tac)
     gene            745..821
                     /locus_tag="KPK67_003821"
     tRNA            745..821
                     /locus_tag="KPK67_003821"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Asp,seq:gtc)
     gene            832..906
                     /locus_tag="KPK67_003822"
     tRNA            832..906
                     /locus_tag="KPK67_003822"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:864..866,aa:Gly,seq:gcc)
     gene            916..989
                     /locus_tag="KPK67_003823"
     tRNA            916..989
                     /locus_tag="KPK67_003823"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:950..952,aa:Arg,seq:tct)
BASE COUNT          353 a          260 c          294 g          327 t
ORIGIN      
        1 attaagatat atgtgtagtt tttagagtgc taactctaaa gaaactagta ttgctaggtt
       61 ctataaactt attaaaaact taatagaaat attaagcata aagattatgt ggttacaata
      121 gcagagagga tacacctgtt cccattccga acacagaagt taagctctct agcgctgatg
      181 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgttaaatg ttccaaggta
      241 gctcaacggt ggagcaaccg gctgttaacc ggtaggctgt gggttcgagc cccacccttg
      301 gagccaaaaa agccgaagtg gcggaactgg cagacgcaca ggacttaaaa tcctgcggga
      361 cttacctctc gtaccggttc gattccggtc ttcggcactt ataaaacaat atcgcggggt
      421 ggagcagttg gcagctcgtc gggctcataa cccgaaggtc gtaggttcaa gtcctgcctc
      481 cgcaaccatt aaaacggctc gttggtcaag aggttaagac accgcccttt cacggcggta
      541 acaggggttc aattccccta cgagtcacca attcaattaa atatgcgggt gtagctcaat
      601 ggtagagttc tggccttcca agccagctgt gagggttcga tccccttcac ccgctccaaa
      661 aatgggacca tagctcagct gggagagcac ctgccttaca agcagggggt cacaggttcg
      721 agccctgttg gtcccaccat tcgcggcctg gtagttcagt tggttagaat gccagcctgt
      781 cacgctggag gtcgagggtt cgagcccctt ccaggtcgcc aaattaaata agcgggaata
      841 gttcagtggt agagcgcaac cttgccaagg ttgaagtcgc gagttcgaat ctcgtttccc
      901 gctccataat aacttgggtg catagctcag ctggatagag caacgccctt ctaaggcgtg
      961 tgtccggggt tcgaatccct gtgcgctcat taagaaaagt ttactataaa ttatagtaaa
     1021 cttttatatt gtttaaaaag acacaggaaa catgtatatt acctgtgtct ttttatttat
     1081 aaactttaaa ctatgtaaaa attacagtat attctaatag agttacagta tattcattaa
     1141 tattgtaaac atacggaggg taatgtataa tagtctacaa gatgtttatg aaggcatcag
     1201 ttaaatataa taaatataac taattagtaa gagt
//
LOCUS       DAEMHW010000357         1205 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.360, whole genome shotgun sequence.
ACCESSION   DAEMHW010000357 DAEMHW010000000
VERSION     DAEMHW010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.360"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..349
                     /locus_tag="KPK67_003824"
     CDS             92..349
                     /locus_tag="KPK67_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460688.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(459..1139)
                     /locus_tag="KPK67_003825"
     CDS             complement(459..1139)
                     /locus_tag="KPK67_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG2460689.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          497 a          149 c          133 g          426 t
ORIGIN      
        1 actaagttta aaaatttaaa taatctataa atcaattata aaatattatt tcttagaaat
       61 taaaatgaat taagtaaatg gaggaattga tatgataata attagaagtc aagataaaac
      121 taatttaatg cagataaacc aaattaaaat agatggtagt caagtttatg cagtatttga
      181 aagtaaaatt gatactgtaa aaataggaga ttatgaaaat aatacaagag ctatacaagt
      241 gttggacaat atacaaaact tcattgaaaa tggaacaaaa tatgattata taacatcaaa
      301 taaagtcaga tacaatgtta ataaaatatt tcagatgcca gctaaatagt gagtgtgcat
      361 tttaatttaa taactaaagc ccactttctt ttgtagtatg aaagagggct ttacatattt
      421 gtaaatatcg aagtacactt attttatcaa taaattattt aataaggtat aatggaccaa
      481 ttaaacaata aaaattctcc taaaacagca agtattaaat atataatagc ttcatttttg
      541 taaagagagg taattttttt gcttatgtca tcatcaattg taatagcaat ctcatcttga
      601 ataatatttt tatatataga tgggaaaata tcttttttct ttataagatt aaataaatta
      661 tttacaccta cccatataaa atatcctaaa acagcaccta atgtgttcat cattaaatca
      721 tcaatatctg tagctcttaa gttaaacaat tgacaaagtt caattgctaa tgaaaaacct
      781 aatccagtta atgaaacatt gataatattt ctaaaatttt tccaaattaa aggtagtaaa
      841 aatcctaaag gcataaacat aattatattt aaaatatatg ttaagatgcc aactgagtca
      901 aaaggtatta gatttatttc atcaattcta aaaagcccac tataccttcc tatatcccat
      961 atgctaccaa ttccagcttt actaagtgct agatagatat ataataaaaa aatatatacc
     1021 catactaaat gtatcattac attcttttca actttcaaat tttttcgttt tataacgact
     1081 atttggtata ttaagcatgg taatataacg caaataaatg aataaaatgt ccatatcatt
     1141 ttattttgtc ccctttcaaa tttatgtttt tagttttata agtactatct ttatattaaa
     1201 ctgta
//
LOCUS       DAEMHW010000358         1183 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.361, whole genome shotgun sequence.
ACCESSION   DAEMHW010000358 DAEMHW010000000
VERSION     DAEMHW010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.361"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(276..983)
                     /locus_tag="KPK67_003826"
     CDS             complement(276..983)
                     /locus_tag="KPK67_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG2460690.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          376 a          210 c          103 g          494 t
ORIGIN      
        1 tttttaaaaa taaatattta gtattaaagt atatatcaca aaattgcatt tatagtttta
       61 actttgaaat atttttaact aaaaaaaaat acccaactga cctaagcaac tcgattgggt
      121 ataaataatc taaaaaatca tttttgtata aactctttat ttaacttaca tttttattta
      181 ccttctactt taattcaact tatatttcta tgcttcttat actctcattc aacttatatt
      241 tctacttatc ttatactttt ataaattcat cacaattatt tagttcttgc tatcaaagaa
      301 attgtattaa aatactttcc attctctact tccatcattt ttttgtcatt tatagcatat
      361 gaattatcac aatttatcca atccttccaa gcttcatcat gacaatttaa agcttcacat
      421 ttctctacaa tcacagactc cgattcagac caaagtttat tccaccatgt aattgaatgg
      481 aagttcatat tatcttgcca aaatggaatg agttcatctg gtatacaatc cacgaaatct
      541 tcttttaatc caggcattgc catagcaagt attcccccct cttttacaag aggtgatata
      601 tgattgtcta aaaatccttc tttgtttcca aaatagtgat aagaatcgat acttatcacc
      661 gcatcaaaga aaccttctgc ataaggtagt tcatgtgcct ctgcctgtat tggaaatatt
      721 ttatcatcca atttaaactc tttaaatctt ttatagtttt cagttggttc tatccataaa
      781 tctgttgcaa atacagtagc atcaaattcc tttgctaaaa atatcgaaga tataccctta
      841 ccacaaccta agtctaaaat acgcattccc ttttctagtt ttatcttact tgttaattct
      901 tccaatattt ttatacaatt aggtcccatc atatttttca tgagaaagtc cttatcgtat
      961 ttattacttt ttgtaaatat cattctatct ttcctttccc tatcagttat tatttattaa
     1021 attttatacg tttgattttt tattttttat atgcttctta tattgagcaa tctctttttt
     1081 attcattctt taacagacaa ttttaatgtg taattttatc taatttataa tagtcacttt
     1141 aatgagtaat cataattttt atatcatttt agatacttac tct
//
LOCUS       DAEMHW010000359         1175 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.362, whole genome shotgun sequence.
ACCESSION   DAEMHW010000359 DAEMHW010000000
VERSION     DAEMHW010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.362"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..838
                     /locus_tag="KPK67_003827"
     CDS             <1..838
                     /locus_tag="KPK67_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG2460691.1"
                     /translation="VVYLIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNIT
                     KDIKLKYSYEVETPAFFNSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFL
                     KTVYWFNPFIYIMDKHIDLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNK
                     FIAGLHKQSDIKKRVMNMFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNILPKG
                     MEEKDVTNSAIVTMIEFTYAEAPKSMRERYKINCEFLGLIPKDSDKIGVGVEYYDSLL
                     ND"
BASE COUNT          489 a           98 c          168 g          420 t
ORIGIN      
        1 agtagtttat ttaatttaca ctatatttaa atatataaaa cttaaaaatt taataaatga
       61 cttatcttat gatgttgatg atagagagat tgtaaatctt tataaaaata tattagaaga
      121 atttaatata acaaaagata taaaactcaa atactcttat gaagttgaaa caccagcatt
      181 ttttaattca tgtgtacttt tgccaccaca tgagtataaa ttgaaagagt tagactggat
      241 ttttagacat gagcttatgc attttaagag tagggacctt tatttaaaat atgtaatcct
      301 gtttttaaaa acagtatatt ggtttaatcc atttatatat ataatggata aacatataga
      361 tttagattgt gaactttatt gtgatgaaag agttttaaaa aatcgaaatt cagaggaaaa
      421 aaaggattat gctttaacaa ttctaaatgc aatgagaaaa ggttcaaata cttcaaataa
      481 atttatagct ggactccata agcaatctga tattaaaaag agagttatga atatgtttaa
      541 tgaaaagtac aagaatggta tattaatggc attaacttta tgtttattat cttctattac
      601 ttttttgaag gttaattcaa cgagtattgt taatcttaat atattgccaa aaggaatgga
      661 agaaaaagat gttactaata gtgcaattgt tacaatgata gaatttacat atgcagaagc
      721 tcctaaaagt atgagagaaa gatataaaat aaattgtgaa ttcttaggtt taatacctaa
      781 agactctgat aaaataggtg ttggtgtaga atattatgac agtctattga atgattaaat
      841 aaatattatt ttaacagata ttaattataa agttttgaat agtaatttgt tgagaaataa
      901 atatatcctt ataaaaatag atacttatat accaagaata aaaaatagtt aagatagtac
      961 acaaggatat acttataaag aacttaaata agtgaaataa attattaatt ataaggttta
     1021 taattttaaa aaattagata aattcaggtg taaaatacta taaaacacaa ttcggaaaag
     1081 tgtatgttga atttcattta aaatggaatt tcaaagttgc tatagtaagt agtttaagca
     1141 ttcttcttat attaatcttt gagaataatg tattt
//
LOCUS       DAEMHW010000360         1129 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.363, whole genome shotgun sequence.
ACCESSION   DAEMHW010000360 DAEMHW010000000
VERSION     DAEMHW010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.363"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            197..907
                     /gene="rgaR"
                     /locus_tag="KPK67_003828"
     CDS             197..907
                     /gene="rgaR"
                     /locus_tag="KPK67_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBG2460692.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          454 a           92 c          187 g          396 t
ORIGIN      
        1 tattttttta cataatatat ctagaaaagt ataggaattt atcgtaattt aagtttaaat
       61 attaaagaga atttctataa gtagtttaat cgacaattta aatttataaa gaaatttaaa
      121 tttgaaagct agtgagtaca gtttggaaat tgaaataaag taaactaaat gatattttag
      181 atttggaggt gatattatgt tcagggtagt tatatgtgat gacgaaaaaa tacaaagaag
      241 catactaaaa gagtttacag aaaaactttt gtctgaaaga tgtctaaatt atcaaatact
      301 ggaattttcc tgtggagaag atttaatatc caaataccca gaaaaaattg atattatatt
      361 tttagatatc caaatgaaag atatcaatgg aattgaggtg gctaaaaaaa taagaaagtt
      421 tgatgaaaaa gtagaaatta tatttacaac ggctttttca gagtatgccc cccaaggtta
      481 tgaggttcgt gcttatagat atttagtaaa gcctatagag tatagtaact ttgcaatggg
      541 tgtaaatctt tgtattgata accttcaaag aaagaaggag cgttatatag ttttaaatag
      601 taaaaaagga tttaatagaa tactgataga ttctatttta tatgttgaaa ctgtaaagag
      661 agatttagtc gtacatacaa taggtagaga ttataaagct aatatgagca tgaaacaagc
      721 agaaaaatta ctgtgtgaaa atggcttttt tagatgtcat acaagtttta ttgttaattt
      781 aagaagaata gaagatctta gggataatat gattactatt aatgaacaat ttatacctat
      841 aagtaagtat agacttaaag atttaaaagt agcattaaca agcttgttat gtgatataat
      901 gtattaattt taaaaaggta attgaaaata tcgtaaaaaa gatttaatgt catcacaggg
      961 ctattgattg aaactgtctt aatatataaa atgttattta tattaagaca gttttattat
     1021 gcaaaatagc agtttataga tttttaatat ggtttaacat gtatatgtag agtttccatt
     1081 ataatagcat gattattaat tagtagcttg attatcaata tactattat
//
LOCUS       DAEMHW010000361         1114 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.364, whole genome shotgun sequence.
ACCESSION   DAEMHW010000361 DAEMHW010000000
VERSION     DAEMHW010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.364"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(254..1000)
                     /gene="cdtR"
                     /locus_tag="KPK67_003829"
     CDS             complement(254..1000)
                     /gene="cdtR"
                     /locus_tag="KPK67_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBG2460693.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT          415 a          138 c          116 g          445 t
ORIGIN      
        1 atatgtcagt acaaaataac aactgaatat tttaaagaga ctatttcgta cttttatgat
       61 ttaatgagct atcttatcga atcataatct aggaacaatt gttataatat ctaatagtag
      121 ttatatatga tatagtcagt atgtgttttt tcacaatatg attattatat agaggattct
      181 tagggattac tctttttgta taaaaaagag atggttcaaa tttttatctt gagacatctc
      241 ctttttctat ttattatgtt ttaataatat tctttaaaat atttctatat gtattagata
      301 tacgacatat atggccatta ctcattttaa tattattttt ctttaggtcg agagaaacaa
      361 tatgatattt attaactaca aaagatttat gtacacggat aaagcatgca tctaaatctg
      421 gtaaagactt taaactttta tagaattcaa atattctaga agtagtcact aattttattt
      481 tatgtggaat agtgcttgtt tcaaaatata taacttcatc aaatgaaatt ttccatagtg
      541 agaaatctga atagatagta aaaaaattag agcgataatt tttttcttta atgtatcttt
      601 tgtgagcaat tttaatacaa gcttttattt tactttcagc aatttccata tcatgtttta
      661 gtataaagtc cataacttct aacctataat cgaagatccc taaaatcatt tctatatgat
      721 ttgtaagaaa tatgattgga ctaatataat ctgattctct aattttttgt gctatccata
      781 atccatcaca taattcatta cttatcttat attttgcgtc tacaactata aaatatattc
      841 ttactttatt tttttcagta agctctttta ataacaaatt tgcattatga tataatctta
      901 caacatcatt atcaaaacct tcttttatta aaatattatt tattttttct tttagtttca
      961 ttccaaaaca aacatcatta tcaaagatta atatatccac taaataccct cctataaaaa
     1021 attcaaaagt aaaaaaacaa aagtatttta tagaaaatac ataaaataac actaggagat
     1081 aaataagatt aaggatagtt agaattaagg tata
//
LOCUS       DAEMHW010000362         1051 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.365, whole genome shotgun sequence.
ACCESSION   DAEMHW010000362 DAEMHW010000000
VERSION     DAEMHW010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.365"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            225..905
                     /locus_tag="KPK67_003830"
     CDS             225..905
                     /locus_tag="KPK67_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2460694.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYVKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
BASE COUNT          461 a           64 c          169 g          357 t
ORIGIN      
        1 ataccaaata aagtgtatat cttaaaacaa tttactagag atacaaggat gctatgtgaa
       61 ttttgaagtg tgcagtttag ataataaata tatataaata ataaagaaaa tggatttata
      121 gtatttaaaa tttcttataa tgataaaaaa tgctattaga aaatgtacta aaaatatttt
      181 tccaattctt tatagaaaaa taatgatatt taggagagat tgtaatgcaa attgatgtat
      241 tgataattga tggagataag aatagttctc aagagctgaa aaatttttta gaaggaaagg
      301 ggataaatgt ctatttagca tataattgta aagatgctat tataaaaata tttagcaaga
      361 aatttgactt aatattttta gagataattt tagttgatgg tgatggttgg gatttttgta
      421 aaaaaattag aaatataact acttgcccaa ttattcatat aacttatata aatgaaaatc
      481 aaagtatttt aaatgcttta aattctggag gagatgatta tttaataaag cctttaaatt
      541 tagaaatatt atatgtgaaa gtgaaagcta ttttaagaag aagagattta tttttaaata
      601 ataataaaaa taatataaaa ctagaagatt ataatagagc tagtggagta ataaagttag
      661 aaaataaaat gataaagtta actccaacag aaaatagatt attaaattac tttatagaga
      721 atgctgaaaa gactctaact attaaggaga tatatgaaca tgtatggatg aatgagtatt
      781 tagatgataa ttatagtgta gtggttgctg tgagtggatt aagaaagaaa atagagaaag
      841 attataaaaa tcctcaaaaa attataacga taagagaagt agggtattac tttaacaaga
      901 tataaagctt tataaacaga atgaatgata gggcatagat aaatatgtta gtatattaca
      961 agagtttttg ttctaacgat atatttgtaa aattatattt aatttactta attgaattat
     1021 agcagtagaa taaatataaa aaatgtctga a
//
LOCUS       DAEMHW010000363         1030 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.366, whole genome shotgun sequence.
ACCESSION   DAEMHW010000363 DAEMHW010000000
VERSION     DAEMHW010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.366"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     repeat_region   104..397
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=237..265
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
BASE COUNT          397 a          148 c          110 g          375 t
ORIGIN      
        1 attttttaga attgtgtcga tattattatg aagaagaatt aagaagaaga aaattaatgt
       61 aaaatatact tttaaataaa aaatagagta gttcaaacta ctctattctt gtccacatag
      121 ttaatataaa actcaaaata gtcagcgata ttttttcaag tatcttggtc atttacatac
      181 catatagtta atataaaact gttgagttta aatgtaaatg attttattaa tataaaattt
      241 acataccaca tagttaatat aaaacttaat catgctgcgc caagcattac attgcattac
      301 atatttacat accacatagt taatataaaa cttagatata tctcattatt tatcttatac
      361 atttgagatt tacataccac atagttaata taaaacctca aaataaacta agtattttca
      421 atatctacac atatacaact cgctcaaatt tgcagtgaac catgagtagt gcaattgata
      481 agatttatca cataccctca atgcctcata tctcaactgt taagccctat tttatcaaaa
      541 atatcgctca ctgcaaaatc tctacatttt tattatatca aaaaaaatat tatttttgaa
      601 tatctgtact aatttgtgat ataataaaag caagaagaac tacaatctat tttgcgttag
      661 agtggagttc atcataaaca gagtttattt tttggattta aatataaatt taaattcaac
      721 tcgtaagtca ctctttgtgc gagagtggct ttttgctttt ttgaatagtt tactgattaa
      781 gtaaactacc acactagctg ttaaactagc taaaacatta aacaaaaagt tatccatgaa
      841 ctcacctccc ttcgaatcgt tgggaggata atcttttgta catgaactcc actctataga
      901 ttgtaagtta aatcttcttg ctacaattat tatatcatat tatggtaaat tatataatta
      961 aaaacacttg tttaaattct attataatta aaataagtac atataaaaat gtacttattt
     1021 ttataaaaat
//
LOCUS       DAEMHW010000364         1024 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.367, whole genome shotgun sequence.
ACCESSION   DAEMHW010000364 DAEMHW010000000
VERSION     DAEMHW010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.367"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>1024
                     /locus_tag="KPK67_003831"
     CDS             <1..>1024
                     /locus_tag="KPK67_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008727275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2460695.1"
                     /translation="TRDLQDIINLAQNLDCYWLYPSVQSEEDYGHYLIEELDELELPE
                     ESKKYFMYEEYGRDAAINDGGRFTEQGYIYNNRNTFTEWYDGRDVPEEYRVTPQPPVQ
                     EKEQADLDAAAAIPTTTTEQPPVLPIILSSEKPADKMKEITDRLEQGILGLYESDHYA
                     DYLRTMSKFHDYSLNNTILIAMQGGNLVKGYKQWEKEFDRHVKPGEKAIKILAPSPFT
                     VKKQVEKIDPDTPKPVFDKDGKPVTEEKEIKIPAFRVVSVFDISQTEGKELPALTYEL
                     TGNVEQYKDFFAALEKTSPFAMGFEALSGGVKGRCNYEEKRIIINEGMDELQNIKTAI
                     HEIAHAT"
BASE COUNT          318 a          244 c          246 g          216 t
ORIGIN      
        1 atacaaggga cttgcaggac attatcaacc ttgcacaaaa ccttgattgt tactggcttt
       61 atccgtccgt acagagtgag gaagattacg ggcattatct gattgaagaa ctggacgagt
      121 tggagctgcc cgaagaatca aaaaaatatt tcatgtatga ggaatacggg agggacgctg
      181 ctatcaatga cgggggcaga tttaccgagc agggctatat ctacaataac cgcaatacct
      241 ttacagaatg gtacgacggg cgcgacgtgc cggaggaata ccgggtaacg ccgcagccgc
      301 cagtacagga aaaggaacag gcagaccttg acgctgccgc agccataccg accaccacca
      361 cagagcagcc cccggttctc ccgattatcc tatcttctga aaagcccgct gacaaaatga
      421 aagagattac cgaccgactg gaacagggga ttttaggact gtatgaaagc gaccattacg
      481 ccgactatct gcgtactatg tcaaaattcc atgattacag ccttaacaat acaatcctta
      541 tcgccatgca gggcggcaat cttgtgaaag gctataagca atgggaaaaa gaatttgacc
      601 gccatgtaaa gcccggagaa aaggctatca agatacttgc gccgtccccg tttaccgtta
      661 agaaacaggt ggaaaaaatc gacccggaca ccccaaaacc tgttttcgat aaggacggga
      721 aacctgttac cgaagaaaaa gaaatcaaga tacccgcttt tcgtgtagta tctgtctttg
      781 atatttcaca gaccgaggga aaggagcttc ctgcccttac ctatgagctg acgggcaacg
      841 tggaacaata caaggatttt ttcgctgctc ttgaaaagac ttcccccttt gctatgggat
      901 ttgaagcact aagcggcggc gtaaaaggac gctgcaatta tgaggaaaag cgtatcatca
      961 tcaacgaggg catggatgaa ttgcagaata tcaagaccgc aatccatgaa atcgcccacg
     1021 ccac
//
LOCUS       DAEMHW010000365         1016 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.368, whole genome shotgun sequence.
ACCESSION   DAEMHW010000365 DAEMHW010000000
VERSION     DAEMHW010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.368"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            38..532
                     /locus_tag="KPK67_003832"
     CDS             38..532
                     /locus_tag="KPK67_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG2460696.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKSDYGSSIDIMNEQNKIDIVIKLLND
                     KSNT"
     gene            921..>1016
                     /locus_tag="KPK67_003833"
     CDS             921..>1016
                     /locus_tag="KPK67_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460697.1"
                     /translation="MHVYQPKTSEYLLNTSAKSYLSSIVFLFDYIT"
BASE COUNT          411 a          116 c          142 g          347 t
ORIGIN      
        1 atttatatat tattaatttt atatggaggg gaaataaatg aaaaataaaa aaggatttac
       61 tctagtggaa ttattagtag taattgctat aataggaata ttggcaatag tagcacttcc
      121 agcattattt aaaaatatag aaaaagcaaa gatagctaaa cttgaagctg atataagtgc
      181 aataaaaagt gcgtctctta gctactatgc agatgaatca aaatatactg atggaggaat
      241 gatatcatgg gtaaagaaag atggaaaaat aataataaat ggtggcttta aagatgaccc
      301 attagcagat aaaatagaaa atttaggtat gccttataat ggttcatatc tattaatgtc
      361 atctcctggt catgaaaaat atctagaatt aagtatactt ccagaaggag aaataagcaa
      421 aagtggtcta gataaattaa aaagtgatta tggaagttca atagacataa tgaacgagca
      481 aaacaaaata gatattgtaa taaaactttt aaatgataaa tcgaatactt aaatacaata
      541 aattttattt aataattgct gatgaaataa acaatcttgt ttggagaaat gaaaaaagca
      601 ctcctcaaga atagaatgct tagttataac tacagaagtg tattggcata cacttctatt
      661 ttttattata tcacataatt taaaaatacc tattcaagtt gtttttaaat tatgttgagt
      721 aatattcttg atagtttata cattaatgaa tttcaaaaaa ataagtactc tttataataa
      781 aaagtacttt agtgtatgtt aatgtcctat ctaataaaat tgtaacatgc cttatacttt
      841 agtatgataa ttttcatttt cttcattgtt gcaacttata ctatagtttt gctaatttat
      901 ctgcaaacac aacttcttta ttgcatgtgt atcaaccaaa aacatcagaa tacttactta
      961 atacatctgc aaaatcgtat ctttcatcta tagtattctt atttgactat ataaca
//
LOCUS       DAEMHW010000366         1008 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.369, whole genome shotgun sequence.
ACCESSION   DAEMHW010000366 DAEMHW010000000
VERSION     DAEMHW010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.369"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(182..721)
                     /gene="scpB"
                     /locus_tag="KPK67_003834"
     CDS             complement(182..721)
                     /gene="scpB"
                     /locus_tag="KPK67_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBG2460698.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIESYQEVATNE"
     gene            complement(723..>1008)
                     /locus_tag="KPK67_003835"
     CDS             complement(723..>1008)
                     /locus_tag="KPK67_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HBG2460699.1"
                     /translation="IFKVKTSQIENANDEKLDKIVRKKIISVEEKILYIRDIIKEKIE
                     VTFTNIIKSYENDEIIATFLSILELIKEKEIVVVQDIFFDDILIRKSSEC"
BASE COUNT          306 a          154 c           76 g          472 t
ORIGIN      
        1 aataattcat taagctccat gtttatatta tcatcaaaaa attactattt atacttaatt
       61 atccatattt attttaagag aattataatt ttatatttat acaaaaaaac acaacaactt
      121 agagcatttt aaatctttta aaatttaaaa ttacatacta aattattgtg attttatatt
      181 tttattcatt tgttgccact tcttgataac tttctatagg tggcaattct tcaagacttt
      241 ctatattcaa caactttaaa aattcatctg ttgttttata aattattggt ttccctatct
      301 tattcaatct accagcttct cttataagtt cattttcaaa caaagtttgt aataccttat
      361 cacattttac tcctcttata tcctctattt ctacctttgt tattggttgt ttgtaggcaa
      421 ttatagtaag agtttctaat gtagcttggc tcaaactttt ctttttcttt ggttcaataa
      481 tcttctttat atattctgca tagtctttat ttgtacacat ttggtactta ttctctaact
      541 tgattatttg aatacctcta ttttgttctc tgtactcctc aataagtaaa tttaacataa
      601 tctctatttc ttttgatgaa agttctttat ttattatata gtttaattct tttatactta
      661 ttggttcacc atatgcaaac ataacagatt ctataatata ctttatatct tctcttttca
      721 ttttaacact ccgaactttt tcttattaga atatcatcaa aaaatatatc ttgtactacg
      781 actatttctt tttcttttat aagctctaat atagataaaa aagtagctat tatttcatca
      841 ttttcatatg attttataat atttgtaaaa gtaacttcaa tcttctcttt tataatatct
      901 cttatatata atattttttc ttctacagat attattttct tccttactat tttatctagt
      961 ttctcatcat tagcattttc tatctgtgat gttttaactt taaatatg
//
LOCUS       DAEMHW010000367          989 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.370, whole genome shotgun sequence.
ACCESSION   DAEMHW010000367 DAEMHW010000000
VERSION     DAEMHW010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.370"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(150..881)
                     /locus_tag="KPK67_003836"
     CDS             complement(150..881)
                     /locus_tag="KPK67_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2460700.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
BASE COUNT          370 a          150 c           80 g          389 t
ORIGIN      
        1 ataaaataaa acaccaaaat tttgaattaa aatttgtaaa attgattaga aaatagtatt
       61 gcactattac cattaaaatt ctatatacaa taaaagagaa gaaaaaacta tactttaaaa
      121 ataaatcaga agaaaataaa ttaatcatct tatatcctcc tatttttcat gaactcaagc
      181 ataacttttg aaaatatttg ttgacgtagt ctagaaacag gtattctatc tccactttcc
      241 ataactgcat taccattttc atatccatat acatattgta aattgattat ataactacga
      301 tgacacctaa aaaaatttgt tggtaaactt ctctctaaat tttcaatagt ctcataatag
      361 tctacagtct ctttctttaa atgaacataa atctttcttt ttataacttc acaatataaa
      421 atatcagcaa gttttaattt ccaatatgaa taaccttttt gtattgtgat ataattactt
      481 tcattatcat caagttttgt aactatttta tccaaagttt tatatagttt ctcattagat
      541 agaggtttta tcaaataatt tatagcatct acttcaaaag catcaaatac aaattcattt
      601 aaaactgtaa cgaaaattat aaagcactca ttaccatatg accttaattt tctagctgcc
      661 tgcattccgt taatctcttc catctgtata tctaaaaata caatattaaa cttttcatga
      721 ttgcatagta aatcttctac tttattaaaa agctctatat tatataataa attcttctgc
      781 ttcatgtata attctatatt ttcctttaaa tgatttagca tataggattc atcatcacaa
      841 atggcaattg aaatcaatat atacaccttc tttcttatca ttattctaat tattatataa
      901 agttaatttt ttctgttcaa ttaatctggt ttaatatgtt gaatatttgt tccaaaatgt
      961 aataatctca cttttcatag tatatctaa
//
LOCUS       DAEMHW010000368          981 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.371, whole genome shotgun sequence.
ACCESSION   DAEMHW010000368 DAEMHW010000000
VERSION     DAEMHW010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.371"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(154..843)
                     /locus_tag="KPK67_003837"
     CDS             complement(154..843)
                     /locus_tag="KPK67_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460701.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAVVQNIVK"
BASE COUNT          371 a          197 c           68 g          345 t
ORIGIN      
        1 aaaagcattg aacaaataaa aaattgctag aaatctatat tttatagtgt tctagcaatc
       61 ctcatatttt aatattcttt taatattcaa tttatctatc taaacaaaat gttaatttaa
      121 aatacttaac atttcgttga atttattctt aaattactta actatatttt gaacaacagc
      181 ttttttctca ccactcatat acactttgat aaattttata tcatctacta taaatctatg
      241 tttaatctta ctacaatcta atttaaataa gccaaatacc atttgtgtga aataccccaa
      301 tccaaatgca cttattatag ttcctatacc aacttttcca cctaataaaa atcctactat
      361 taatgctcca acttctatag tacctcttat taatttaagt gattttcctg ttttcttttg
      421 aattgcaacc atcaaaccat ctctaggtcc acttcctaaa ccacttccca tataaaatac
      481 cattccaaca gacaaaagaa ccaatcctaa tatcaacata attattccta caaataaact
      541 atcagcctct ggaattaaat tactatataa aagtaaatcc ataaaaaatc caattagtag
      601 catgttaaaa acagttcccc atcctacatt ttctccaaga acgacatcag caataactac
      661 tatagagcct acaattatag aagcagttcc tatagttatt cctatatgat ttgaaagtcc
      721 ttgatgaaat acatcccagg gagataatcc aaggttacaa tttatcgtca atactatacc
      781 aactgaacat ataaataaac cgactactag ctttaaaaat ttccataata ctttttgcaa
      841 caaattctac ccctcctaaa cataatttaa ctactcactc taaatatcaa aagccagcag
      901 ttttcttaca aaaatcttat tttattaatt ttataatata cttctataat acattatttt
      961 atgctaaata actaccattt t
//
LOCUS       DAEMHW010000369          936 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.372, whole genome shotgun sequence.
ACCESSION   DAEMHW010000369 DAEMHW010000000
VERSION     DAEMHW010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.372"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            279..746
                     /locus_tag="KPK67_003838"
     CDS             279..746
                     /locus_tag="KPK67_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG2460702.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          355 a           63 c          158 g          360 t
ORIGIN      
        1 ccttgaaatt aaaaaaacat agatatttca gtaatctaaa atagtctaca aaaataatag
       61 ttttatcaaa attaataaga atacatatag atttaaacct aaaagtatga acatgtctgt
      121 tttttacaag attaaaattt atattatatg ataaaattaa cttaactatt tcaataagac
      181 ttataatttt tttctttata ctagacctta ttgaaacctt tatagtttaa ttctaattat
      241 atattataaa taaagtatgt gtatattaag gaggtaatat gaagttttta aatgttttaa
      301 tagtagttga agatattgag aaatcaaaaa agttttatta tgatgttttg gggttaaagg
      361 taatctgtga ttttggagaa aatgttgtat tagaaggtaa tatatcatta caagaaaaaa
      421 agctttggtt agaatttatt aataagagtg acagtgaagt taagttcaat ggaaatgatg
      481 ctgagttata ttttgaggaa gataattttg atacttttgt tgaaagattg agtacaatga
      541 aagatataga ttatgttcat ctagctatag agcataggtg gggtcaaaga gcaattagat
      601 tttatgattt agatgggcat attattgagg ttggagaaac tatgtcatct gtgtgtagac
      661 gttttttaga tagtggtctt tcaatagatg aggtagctaa aagaatggat gtaactgtcg
      721 aatatattga atcagtttta gaatagatgg attatattaa agtaaaacta tattttgtat
      781 agaagtaagg gctgtaagta aacttgtatg gaaacaaatt tacttacagt ttttgttttt
      841 gtaagataca gaatataata tttgttataa ttttatttgg atttttaaaa agtgcatgat
      901 tttccttaca tattataata gaatttaata tttatt
//
LOCUS       DAEMHW010000370          908 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.373, whole genome shotgun sequence.
ACCESSION   DAEMHW010000370 DAEMHW010000000
VERSION     DAEMHW010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.373"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            208..>908
                     /locus_tag="KPK67_003839"
     CDS             208..>908
                     /locus_tag="KPK67_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG2460703.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEALDTIL
                     NLSKDGILFTSKDGE"
BASE COUNT          391 a           77 c          126 g          314 t
ORIGIN      
        1 aaatatacat acagtttaag ttttaagcaa caattagggg gatttggtga caagattttt
       61 aattataaga aagaaagtgt cttaatgcaa cttttataaa gtcattatag acactttctt
      121 ttttatttga taaatttaaa ttatcaaaat tatattgctt tatacaacta ttttgttgga
      181 aactatttat tatataagga gaaataaatg aaaaaatcag tagctctggt aaatgatagt
      241 agaaaagatt taatagactt tttagaaaat aatttgaaat tagtttttgg agattctata
      301 aatattaata gatacttcat aaatgaaata aatgataatg atattataaa tgatgatgtg
      361 atattagtaa tgtcagtaga aagattagac aaaattataa ataatatact agataagaaa
      421 aaagtgattg tggtaagaag aacttttagg gaagataaaa tttataatct actatcttta
      481 ccacagggta caaatgttct aatcgtaaat gattctgatg aaactactct agaaactata
      541 agtttatttt ataaaattgg agtgactaat attagaccaa ttccatatat gaatgataat
      601 aactataaaa atataaaaat agctataact ccaggagttc cagaaaaggt acctagcttt
      661 atctcagata tatttgattt aggtcacaga tatatagata tctctacatt tatagaaata
      721 ataaatttat tacaaattga ttcaaaggaa atacaatcta atctagttaa atactcagaa
      781 gaaataataa gtttagatac tggtataaaa gataaatata aggaattatt tttaaaaata
      841 gaagcattag atacaatatt gaatttatca aaagatggta tattatttac ttcaaaagat
      901 ggtgaaat
//
LOCUS       DAEMHW010000371          877 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.374, whole genome shotgun sequence.
ACCESSION   DAEMHW010000371 DAEMHW010000000
VERSION     DAEMHW010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.374"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            228..719
                     /locus_tag="KPK67_003840"
     CDS             228..719
                     /locus_tag="KPK67_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBG2460704.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          342 a           78 c          141 g          316 t
ORIGIN      
        1 tttaaaagtg atagttctta tttatagtca atattgccta tagaacttct gattcattta
       61 atttttagga aaaaataata atacttacgc tataaacaca gaattttagt tgataatata
      121 ggtatttaaa tttaagatga aataatactt ttctcaatat attttattgt aactaagttt
      181 aattataagt tacaattaaa aaaacgctac ttaggaggaa tgtaattatg gggatgatag
      241 gatgttatac taaaataagt gaagagaatg tttttaaatt acagcaagca gaagaaaact
      301 tacaagattt tgtttttgaa gatgccaacg aaaatagtac tatcaatatt gataaagcat
      361 ggcatgccat tcactttact ttgacaggtt gtccttttgg tggagatgat gataatatat
      421 ttagcaaatt agtactaagt ggaaacatac ttatggaaat tgatggagaa tttcctgtca
      481 tgttgattac agcaaatgat gtaaaaaaac tatcaaaagc aatgaacagt ttggaagaac
      541 aagcatttag aaaaagattt aatattaatg agatgttaga aaataatatt tatcctgtta
      601 tgaatgatga gagtgaagat gatttttttg aatatgtatg ggcaaatcta attgagctta
      661 aaaagtttat acaagaagca tctgatgatg gactagctgt aatatttttt ataatgtgat
      721 aattactaaa aaattgtgaa tattagtgtc ataacatatc ttagatatga agccttttga
      781 ttgtatagaa gaaaatacat attatattag ccttttaaaa tatataagtt aaatttaaat
      841 ctgtaggtaa atttgtctta tgtctttata aatattt
//
LOCUS       DAEMHW010000372          876 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.375, whole genome shotgun sequence.
ACCESSION   DAEMHW010000372 DAEMHW010000000
VERSION     DAEMHW010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.375"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            91..792
                     /locus_tag="KPK67_003841"
     CDS             91..792
                     /locus_tag="KPK67_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG2460705.1"
                     /translation="MNNSFLDKLIETKELKNSLYNVLKHNFLYHSNKIEGSTFTTEAL
                     ALLLDKNVVTGRHTLDDVQETVNSSYVFDTIIDSLKEKITHNFLRNLHSSLMFNTTLH
                     SRGMSGIYKTIPNMILGTDVSIAQPFEVEPKLDELIEWYYSQSEVSIKVIAEFHYRFE
                     LIHPFQDGNGRIGRFVMLKQMLENNLPIKIVSWDSEDLYRNSLNSCSLGNYVPLIEYL
                     SSLEDFREVYKMLWK"
BASE COUNT          328 a          100 c          135 g          313 t
ORIGIN      
        1 attaaattaa atctgaactt tacatactat tagaataagt attataagat tataatgatt
       61 taaaaatatt aaaataattg aggtgataac atgaacaatt cctttttaga taaattgatt
      121 gagacaaaag aacttaaaaa ttcactttat aatgtgttaa aacacaattt tctttatcat
      181 tctaataaga tagagggcag tacttttact actgaagcat tagcactttt actcgataag
      241 aatgttgtta caggaagaca tactttagat gatgtacaag aaactgttaa ttctagttat
      301 gtatttgata caataattga ctcactgaaa gagaaaataa cacataattt cttaaggaat
      361 ctacattcaa gtttaatgtt caatacaacc ctacattcca gggggatgtc tggtatatat
      421 aaaactatac ccaatatgat actaggaact gatgtaagca tagcacaacc atttgaagtt
      481 gagccaaagc ttgatgaatt aattgaatgg tactatagtc agagtgaagt atctattaaa
      541 gttatagctg aatttcatta tagatttgaa ttgatacatc catttcaaga tggtaatggt
      601 cgcataggta gatttgttat gctaaaacag atgttagaaa ataatcttcc tataaaaatt
      661 gtatcttggg atagtgagga cttgtataga aactccttaa attcttgtag tttaggtaat
      721 tatgtccctt taattgaata tttatcaagt ttagaagatt ttagagaagt atataaaatg
      781 ttatggaaat aattgtttgc agcaaaaaaa gtttttatat aaatatattt ttatctatgc
      841 tatttaacgc tgatttctta gttattctta gcttgg
//
LOCUS       DAEMHW010000373          840 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.376, whole genome shotgun sequence.
ACCESSION   DAEMHW010000373 DAEMHW010000000
VERSION     DAEMHW010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.376"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            57..416
                     /locus_tag="KPK67_003842"
     CDS             57..416
                     /locus_tag="KPK67_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBG2460706.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            469..>840
                     /locus_tag="KPK67_003843"
     CDS             469..>840
                     /locus_tag="KPK67_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBG2460707.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIK"
BASE COUNT          371 a           74 c          131 g          264 t
ORIGIN      
        1 tagagagaaa aataaatttt attagatgaa acttttgaaa aggagagaat atttaattga
       61 taaatttgat aaataaaaaa cgaaaaggat ttacacttgt tgaaatgatt gtagtagtaa
      121 ctattttagg cgttatatct agtatagcat tagttaagta tagtaaggtt caagaaagtg
      181 ccaaattaaa tgcagactat acgaatgctg ctaatatagt aaccgcagct agcatggcaa
      241 ttaatgatga tgaaaagaca atagactctc taagtgtaga gacattgaag gaaaagggat
      301 acctaaatac tgttccagtt cctcagagta catcaggtaa attcgaactt gtcataaatg
      361 atagcggaac agatataagc gtaaatataa attctaagca attttatcca aaataatatt
      421 ttatattgat tgctgtaagt tttaaaatta tttttattta agaaggagat ggctataatt
      481 aaaaaaattc taaacttaga aataaatgat gattatataa ggcttgtatt tttaagaaaa
      541 agtcataaaa aagtttttat agataaatca attataaaag agattaactt tgatataaaa
      601 aatgatagta attcgattga gaagattaaa tatatagaag atgtagttag atttgtaaaa
      661 gaggagattg gtaaatcaaa aatattatat aaaaatttat actttaacat tcaaattcag
      721 gatatagtca ttagaaatgt tgaagttcta aatactaaga aaaaaagaga catattaagc
      781 atgattgaat ttgaaataac acaatacatt ccaataaaca taaataacta ttcaataaaa
//
LOCUS       DAEMHW010000374          807 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.377, whole genome shotgun sequence.
ACCESSION   DAEMHW010000374 DAEMHW010000000
VERSION     DAEMHW010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.377"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            214..354
                     /locus_tag="KPK67_003844"
     CDS             214..354
                     /locus_tag="KPK67_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460708.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(351..722)
                     /locus_tag="KPK67_003845"
     CDS             complement(351..722)
                     /locus_tag="KPK67_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG2460709.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
BASE COUNT          288 a           92 c           97 g          330 t
ORIGIN      
        1 acaataatag atttttttgt tattattacg agttttttat tgcaactgta gaataaaagt
       61 attattatat aattaagata attatctagg gaaattttgg aaaatcaaaa tatattttat
      121 aaaatcaaaa tacaattaca taaactttat taaaaatttg ttgggaattt ctgactaaca
      181 ttaagaacat aagtttgttg ggggatggta tatgtggaaa agtcaaacac tactgttaca
      241 aaaaaagaaa aaattgaaga tataggaatg aaattagaac atttattgag tgaagatatt
      301 actttttatg aattattact tgagattttg tataaagaaa aagaagagaa ttaattctct
      361 tctttaagga atctttttaa ataggcatta tcaatcattt tttctatagc ttctctatct
      421 tctttttcaa gagaatatat tttttctaag agcttttttg ttttttcact tactgataag
      481 ttctctatga ttaatttatc ttctttgctt atttttgtat tatcatttac gcctaataaa
      541 taatctgttg atgtttctaa tgcttttgct aatctaacta ttatttcact tttaggttct
      601 cttaaattat tttcataacg tgacaagcta gcctcagtta taccgacttt agtagcaagt
      661 tctttttgat ttatatctaa ttcttttcgt agatttgcta ttctatctcc taaactttcc
      721 atatgtacat cactccttac cgaaattata agttattttg ataaaaagta aaataaaatt
      781 accaaaaata taaaaaaaaa gggttaa
//
LOCUS       DAEMHW010000375          804 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.378, whole genome shotgun sequence.
ACCESSION   DAEMHW010000375 DAEMHW010000000
VERSION     DAEMHW010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.378"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          300 a           67 c          107 g          330 t
ORIGIN      
        1 ctatcaaata atatatatcc taaagtgaac cagataaatt ttaaaaatag actgaactct
       61 atttgatttt gagttattat ataaatatgc tcttttattt tattcataga gtaccataag
      121 ataaaatcta aaatagaagc tattaaaata ctaattaact tataggtcat atttgcaaat
      181 attatatctg gacttgtatt tttaacaagt gatattgtac ttacagtaat aaggtgtaaa
      241 atacaaaaac ttattactgc tatattggga ataaaatggt tgttagtaga actttttctg
      301 cttaagagca atttatgact tactatgtaa aacaatatat aatatagtgg taatggtaat
      361 tttgtgaata ttattatcaa gaaggttaat ggttttatta aaaaaatata tttgtaattc
      421 ttatgtatag ttatattttt gtttctaaaa gatgctattt tgcatatgta aatgttacat
      481 agttcgatta ttaatatagt agttatgatt aaaaatatat ggtatttcat agtacatcct
      541 cttttaaagt gaaattgtat ttacttaaaa tagattagat tttatatatt ctattttaca
      601 gtttttattt aggtacatgc aaagagctta ttatggatac cttctttttt attgcatgtg
      661 tctgtgttat ataaaattta aaaagtatta tatcttaata tacagtatgt atgataacta
      721 tgtgtccaaa tatagtaatt atgtatggaa agtataatag ttatgtgtat aagtatagta
      781 attacatata gcatgtatga taac
//
LOCUS       DAEMHW010000376          788 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.379, whole genome shotgun sequence.
ACCESSION   DAEMHW010000376 DAEMHW010000000
VERSION     DAEMHW010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.379"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>788)
                     /locus_tag="KPK67_003846"
     rRNA            complement(<1..>788)
                     /locus_tag="KPK67_003846"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          239 c          182 g          195 t
ORIGIN      
        1 cgtccatccc ggtcctctcg tactaaggac aggtcttctc aaatttccta cgcctgcgac
       61 ggatagggac cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaatggg
      121 cgaacagccc aacccttggg acctactaca gccccaggat gtgatgagcc gacatcgagg
      181 tgccaaacct ccccgtcgat gtggactctt gggggagata agcctgttat ccccagggta
      241 gcttttatcc gttgagcgat ggcccttcca cgcagaacca ccggatcact aagtccgatt
      301 ttcatccttg ctcgacctgt atgtcttgca atcaagctct cttttgcctt tacactctac
      361 gtacgatttc cgaccgtact gagagaacct ttgagcgcct ccgttacctt ttgggaggcg
      421 accgccccag tcaaactgcc cacctgacag tgtcccaaga ccagattcat ggcctatggt
      481 tagagtccca gtactacaag ggtggtatcc caaggatggc tccgccaaaa ctggcgtcct
      541 ggtttcaaag cctcccacct atcctgtaca tgtagtacca agacccaatg tcaagctaca
      601 gtaaagctcc atggggtctt tccgtcctgt cgcaggtatc cggcatcttc accggaatta
      661 caatttcacc gagtctgttg ttgagacagt gcccaaatcg ttacgccttt cgtgcgggtc
      721 ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt tatagttacg gccgccgttt
      781 actggggc
//
LOCUS       DAEMHW010000377          777 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.380, whole genome shotgun sequence.
ACCESSION   DAEMHW010000377 DAEMHW010000000
VERSION     DAEMHW010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.380"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(444..620)
                     /locus_tag="KPK67_003847"
     CDS             complement(444..620)
                     /locus_tag="KPK67_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460710.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT          279 a           82 c          106 g          310 t
ORIGIN      
        1 taaaatgctt gtttttcttg tattatttat ataaatattg aaatttgtct agttatacgt
       61 ttttattgac cagttatgca gttgatattg aatattctaa atattggtgt tattttatag
      121 gtggtttaaa catattaggg ggattttata tatgatttat agagaatgta taaaatagta
      181 aaggtttaga gccatgttta gtggattgca gaatatgcat atagaatgat tcttgtaaat
      241 taagttagac ataggaaata tataatttgt attggatgat tgattttatg gtgtatgctt
      301 ttaacttcta taatgggaaa gtaaagatgt actcccaata catctttact ttagcagaag
      361 ttatagttac ttataaagta caaaaaatta tatatttatt agagtagata aatctaaaaa
      421 ggtttttcta gaaaaattta gttttaattg tatctactta ctaataataa gacttgatgt
      481 aagcaggttt caactaccgt ttctccatat tctgatataa aatcatctaa ctgtgtatct
      541 aaaaaattac aataatcata tgaactactg atgtaagcag ttagtatatg tgtacatttg
      601 tctatttctc caataaacat attatccatc cccctaaaaa atttagtaaa aataaaaagt
      661 cgcattctat tagtcaatta acataattta taaaattttt tagtactagt ttatataact
      721 taactcctta aggttaaata ttcccagttt taccaattta gttttattct acaagta
//
LOCUS       DAEMHW010000378          771 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.381, whole genome shotgun sequence.
ACCESSION   DAEMHW010000378 DAEMHW010000000
VERSION     DAEMHW010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.381"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..390)
                     /locus_tag="KPK67_003848"
     CDS             complement(<1..390)
                     /locus_tag="KPK67_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HBG2460711.1"
                     /translation="MKAIILAAGLGTRLKPLTSECPKPLVKVNGKPMIETQIECLLEK
                     GIKDIKVAVGYLKEKFYYLAEKYNVELIYNNKYDIYNNIYTMYLLREHLPDSYVIEGD
                     VYICNNFIDPDIKESTYFTLYKENFNNE"
BASE COUNT          267 a          110 c           60 g          334 t
ORIGIN      
        1 ttcattgtta aaattttctt tataaagagt aaaataagtg gattccttta tatctggatc
       61 tataaagtta ttacatatat atacatctcc ctctataaca tagctatcag gtaaatgttc
      121 tcttaataaa tacatagtat aaatattatt gtatatatca tatttattgt tatatattaa
      181 ttccacatta tatttttccg ctaaataata aaatttttct tttaaataac ctactgcaac
      241 ttttatatct tttatcccct tttctaatag acattctatt tgggtttcta tcattggctt
      301 cccattaact ttaactaatg gttttgggca ctctgaagtt aatggtttta atcgtgtccc
      361 cagcccagct gctaaaatta ttgctttcat atttaatcct accttccaat tgaatattgt
      421 aatgttttag aggttttaag ttagtgtcac ttataaaatc tccaaatatt ccgatagaaa
      481 ttttttatta gtatatatta aatttcattt taaacctaat gtttattttt atttattcta
      541 aaggtctaaa tttaatctga attttaactt atcatcttta atataattca tcaaacttta
      601 tataattcaa actaataatt acctatcctt ttatttatcg gacaatactt tttaaaactt
      661 atattattaa tataaaaaat cttttaaatc aaaaaatact ttaatatctt ttttgatatt
      721 aaagtatttc tccaaataat caattcctaa tataaaacac attgtaaata a
//
LOCUS       DAEMHW010000379          759 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.382, whole genome shotgun sequence.
ACCESSION   DAEMHW010000379 DAEMHW010000000
VERSION     DAEMHW010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.382"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>759
                     /locus_tag="KPK67_003849"
     CDS             <1..>759
                     /locus_tag="KPK67_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG2460712.1"
                     /translation="SLRDTVKDYQEELRDGIAWVAFWKTGRSWNAEYFRLEMGDILYQ
                     EDRSRMEEIKQADPAAVVVNGYYSGYLGEDMNLDELTAGVRRHYENGYSNIGEFIEAH
                     DDRLPPELIEEARAAAHAAGLPFSEKAYRDGEEPDPYIFDGSMSMEDYELMHRMIENE
                     RSERMAETILSGYLSNLGKYTEGRPAGEWVSFPTTAEHLKEVFDRIGIDGKNYGELHI
                     TEYQSSIEGLAGKLTELESLDELNYLSELLKMQFD"
BASE COUNT          215 a          174 c          212 g          158 t
ORIGIN      
        1 agtttacggg acaccgtaaa ggactatcaa gaagaattaa gggacggtat cgcttgggtg
       61 gcgttttgga aaacgggacg ttcttggaac gccgaatatt tccgccttga aatgggcgat
      121 attctctacc aggaggacag aagccgcatg gaagaaatca aacaggctga ccctgccgcc
      181 gttgtggtaa acggctatta ttccggctat cttggcgagg atatgaacct tgacgagctg
      241 accgccgggg tgcgccgcca ctacgaaaac ggatatagca atatcgggga atttatcgaa
      301 gcccacgacg acaggctgcc gccggagctt atcgaggaag caagagccgc cgcccacgct
      361 gcggggcttc ctttctctga aaaagcctac cgggacggcg aagaacccga cccctatatt
      421 tttgacggga gcatgagcat ggaggactac gaacttatgc accgcatgat tgaaaacgaa
      481 aggagtgaac gcatggcaga aacgatttta agcgggtatc tttctaatct tggaaagtac
      541 accgagggca gacctgcggg cgaatgggta tcattcccca cgactgccga gcatttgaaa
      601 gaagtctttg accgtatcgg gatagacggc aaaaactacg gggagctaca tatcacagaa
      661 taccagtcct ctattgaggg actggcagga aaattgaccg agcttgaaag ccttgacgaa
      721 ctgaattatt tgagcgaact tttgaaaatg cagtttgac
//
LOCUS       DAEMHW010000380          743 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.383, whole genome shotgun sequence.
ACCESSION   DAEMHW010000380 DAEMHW010000000
VERSION     DAEMHW010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.383"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>743
                     /locus_tag="KPK67_003850"
     rRNA            <1..>743
                     /locus_tag="KPK67_003850"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          205 a          153 c          226 g          159 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gat
//
LOCUS       DAEMHW010000381          735 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.384, whole genome shotgun sequence.
ACCESSION   DAEMHW010000381 DAEMHW010000000
VERSION     DAEMHW010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.384"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(91..>735)
                     /locus_tag="KPK67_003851"
     CDS             complement(91..>735)
                     /locus_tag="KPK67_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG2460713.1"
                     /translation="FFYNYNFEYNIKEFENGGDLLKKYPNNVDIIFMDIDLNDENGFN
                     VSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRYLEKPLKYSLFKEAISSYLKECYK
                     NEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHTEKGIYTFKSSMKEIESNLEEHNF
                     FRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSRYKIKLLREKLNSCLVDVII"
BASE COUNT          260 a           98 c           52 g          325 t
ORIGIN      
        1 tttaactata attagttttt attataaatt ttttcatatt ttacaataga tatattctat
       61 tagaatagaa tataagggca aaaatttacc tcaaattatt acatctacca aacaactatt
      121 taacttttct ctcaataatt ttatcttata acgacttaca attattgaat tttgatttat
      181 aaatacaata ttattttcta tatctagcat atttaccttc attaaattta taaggtagct
      241 tttatgacat ctaaaaaaat tatgttcttc tagatttgat tctatttctt tcatactact
      301 cttaaaagta tatattcctt tttcagtatg tatagttaaa tcatttttta aaacttctaa
      361 atataaaata tcatctacct ttatgaaata tatcttgcat ttacatttta tagataaaat
      421 agcttcattt ttgtagcatt cttttaaata tgatgatatt gcttctttaa atagagagta
      481 ttttaagggc ttctctaaat atctataaac tcctatctca taacctttct ttatgtattt
      541 atttgatgag gatacaaata taattcttac actttcatca aattttttga tttcttttga
      601 aacattaaat ccattttcat catttaagtc tatatccata aatatgatat caacattatt
      661 aggatacttt tttaataaat cgcctccatt ttcaaactct ttgatattgt attcaaaatt
      721 gtaattataa aaaaa
//
LOCUS       DAEMHW010000382          708 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.385, whole genome shotgun sequence.
ACCESSION   DAEMHW010000382 DAEMHW010000000
VERSION     DAEMHW010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.385"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..598
                     /locus_tag="KPK67_003852"
     CDS             92..598
                     /locus_tag="KPK67_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBG2460714.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          289 a           71 c          124 g          224 t
ORIGIN      
        1 gacaatcaaa agtcagtatt ataagagtac atatattcaa atttttaaca attaatttat
       61 ataattaaaa aatgaagggt aggatgaagt catgggtttt agagaagtaa aaattgagga
      121 attacagttt aatccattta ctaagattgg caaagaatgg ttacttataa cagctggtga
      181 ttcagaaaag tttaacacaa tgactgctag ctggggtggt gttggagtct attggggtaa
      241 aaatgttgtt acaacttaca taagaccaca gagatataca aaagaatttg tagatagtaa
      301 tgatacattt acagttgcct tctttgatga aacgtataga gaggctttaa atatttgtgg
      361 tactatttct ggtagagata taaataaaat agaaaaagca ggacttacac catattttgt
      421 ggatgataca gtagcttttg aagaagctaa tatgattata gtatgtaaaa agctatatca
      481 tggtaatatg ccaccagaaa actttgatgc taaagaaaat gataaaaaat ggtatccaga
      541 gaaagactac catactatgt acatttcaga aattataaaa gttcttgtaa aagaataatt
      601 ataataaaaa atatatgaaa agaacttatt ataagtaaat gactgtgctt tcttatctga
      661 tatctaagta gatgctagta actaaataaa tattaatttt aaataaat
//
LOCUS       DAEMHW010000383          706 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.386, whole genome shotgun sequence.
ACCESSION   DAEMHW010000383 DAEMHW010000000
VERSION     DAEMHW010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.386"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            172..540
                     /locus_tag="KPK67_003853"
     CDS             172..540
                     /locus_tag="KPK67_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBG2460715.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT          305 a           58 c          112 g          231 t
ORIGIN      
        1 taaagtaact ttataaataa aaagtaatat aaattgataa aattataaat ttatttatag
       61 tgaatatagg ctggatttta tagatacaaa aatagtatat atgatatgta gaccattagc
      121 atgtttaaag tagcaaaaca tataaaataa aatttgaaag gaagtataaa aatgacaaga
      181 ccatcaatat atcatagcca aggatacact tgtgcagagg cattaataaa gtcttataat
      241 gaggaacaca acacagatat accaatctca ataggaagtg gtatgggggt tggaatgaat
      301 gttggaagtg tttgtggagc tgtaaatgct gctgttgtta taatagggta tttaaatggg
      361 cgaaatagta atttagatga aaatatagca aggaagtatt caagagagct attgaataga
      421 gtaagaaata aatacagctc agaaatatgt gtatctctta aaaaaaataa agtaacttgt
      481 gaagaaataa taaatttcac atatgatgct ctaaatgata tgctaaaaaa tcaaaaataa
      541 ttctaagaaa caagtatttt acattcgata gaatacttgt ttttttatga tatttttaat
      601 aagtattagt ttattataat ttttaaatca attttattat attttaattg aaataatcta
      661 ctttgaaaat tactaactga caaaagaata tgattatagt atagaa
//
LOCUS       DAEMHW010000384          699 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.387, whole genome shotgun sequence.
ACCESSION   DAEMHW010000384 DAEMHW010000000
VERSION     DAEMHW010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.387"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>699
                     /locus_tag="KPK67_003854"
     CDS             <1..>699
                     /locus_tag="KPK67_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HBG2460716.1"
                     /translation="SIDFSISLVSKNQVKVNGLYLNESVYSSYLDFVKNSDGHHNTSN
                     FMNLFLDNISFWKLFGFENINFVIDKYFTLVGKTNLGYVEFICDNNKNIDIYFGEWKT
                     SSSKSTIFSGNGRNVVVEPIYNPDTGEDISTSLDFSYEPLYGIDRYINKVLIAPDLYT
                     SLININTNYYSNEYYPEIIVLNPNTFHKKVNINLDSSSFEYKWSTEGSDFILVRYLEE
                     SNKKILQKIRIKGI"
BASE COUNT          272 a           78 c           99 g          250 t
ORIGIN      
        1 aaagtataga tttctctatt tctttagtta gtaaaaatca agtaaaagta aatggattat
       61 atttaaatga atccgtatac tcatcttacc ttgattttgt gaaaaattca gatggacacc
      121 ataatacttc taattttatg aatttatttt tggacaatat aagtttctgg aaattgtttg
      181 ggtttgaaaa tataaatttt gtaatcgata aatactttac ccttgttggt aaaactaatc
      241 ttggatatgt agaatttatt tgtgacaata ataaaaatat agatatatat tttggtgaat
      301 ggaaaacatc gtcatctaaa agcactatat ttagcggaaa tggtagaaat gttgtagtag
      361 agcctatata taatcctgat acgggtgaag atatatctac ttcactagat ttttcctatg
      421 aacctctcta tggaatagat agatatatca ataaagtatt gatagcacct gatttatata
      481 caagtttaat aaatattaat accaattatt attcaaatga gtactaccct gagattatag
      541 ttcttaaccc aaatacattc cacaaaaaag taaatataaa tttagatagt tcttcttttg
      601 agtataaatg gtctacagaa ggaagtgact ttattttagt tagatactta gaagaaagta
      661 ataaaaaaat attacaaaaa ataagaatca aaggtatct
//
LOCUS       DAEMHW010000385          675 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.388, whole genome shotgun sequence.
ACCESSION   DAEMHW010000385 DAEMHW010000000
VERSION     DAEMHW010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.388"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            148..363
                     /locus_tag="KPK67_003855"
     CDS             148..363
                     /locus_tag="KPK67_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460717.1"
                     /translation="MRILSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            369..662
                     /locus_tag="KPK67_003856"
     CDS             369..662
                     /locus_tag="KPK67_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBG2460718.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
BASE COUNT          296 a           80 c          101 g          198 t
ORIGIN      
        1 atatcctaca aaattatatt aatatattaa gtcgactaag ctttgatata aagagaatat
       61 aaaatactgt aaaaaagtgt aatggttatt tccttgaaaa gaaagacata ctattaaagc
      121 ataaattaca atataggaga tgattaaatg agaatacttt cagaaactga aaaaatatct
      181 ttagcagcag ttataaaaat ggagtctgac ggattactta tgcaaagagc aataaatatg
      241 ttaatatcag atgaagacct aaaaagacaa tcagaatcta gtatattagc aacagaagga
      301 agaattaaag ctatacaaca atttatagtt gaaaatgaaa tactaatttc tgaggaggta
      361 tagataacat gagaaatatg atagaaaatc taattaagaa caatacttat atagacgata
      421 aagtaataat gttaagtatg ctttcatctg ctaaagcatc tgccaatatg tatcttaatt
      481 ctgcattgac aagtagtaca cctgaattaa gagctatata ttcagcttca ttaactcaaa
      541 tggtagaagg tcacacagca cttacagaat taagtattaa taaaggatgg gttaaaccat
      601 atgataaacc tattcaacag ttaacatgta gttataaaga atcccaaaat gttattgatt
      661 aatctgaata aaata
//
LOCUS       DAEMHW010000386          664 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.389, whole genome shotgun sequence.
ACCESSION   DAEMHW010000386 DAEMHW010000000
VERSION     DAEMHW010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.389"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>664)
                     /locus_tag="KPK67_003857"
     rRNA            complement(<1..>664)
                     /locus_tag="KPK67_003857"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAEMHW010000387          654 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.390, whole genome shotgun sequence.
ACCESSION   DAEMHW010000387 DAEMHW010000000
VERSION     DAEMHW010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.390"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..349
                     /locus_tag="KPK67_003858"
     CDS             92..349
                     /locus_tag="KPK67_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG2460719.1"
                     /translation="MDNLISFIPEQLLILVAALYVIGAGCKKYKQLDNKYIPVVLLVL
                     GVGFSIWMLGLNPSAVLQGVICWGVAIGINQTYKQLKEENK"
     gene            349..>654
                     /locus_tag="KPK67_003859"
     CDS             349..>654
                     /locus_tag="KPK67_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2460720.1"
                     /translation="MKIGVNCGHTKTGAGSGAIGKINESIETRNVGYKVIDKLKTLGN
                     NVVDCTIDKASTQSECLSKIATQANRQDLDWFISIHFNAGKGRGCEVYTYKGKQYQDA
                     "
BASE COUNT          258 a           69 c          138 g          189 t
ORIGIN      
        1 tggctaattg gaacaatgat tacaaccctt gtagggttct ttatatttgc catacagaaa
       61 ggaatatttt aattaattag gaggataaga aatggataat ttaataagtt ttataccaga
      121 gcagttacta attttagtag ctgctcttta tgttatagga gcaggttgca agaaatataa
      181 acaattagac aataaataca ttccggtagt attattagta cttggtgtgg gtttctcaat
      241 atggatgtta ggattaaatc ctagtgcagt cttacaaggt gtaatttgtt ggggagttgc
      301 aataggtata aatcaaactt ataaacagtt gaaggaggaa aataaataat gaaaataggt
      361 gtaaattgtg gacatacaaa gacaggggca ggaagtggag ctatagggaa aataaatgaa
      421 tcaatagaaa ctagaaatgt aggatataaa gtaatagata aattaaaaac tctaggtaac
      481 aatgtagttg attgtactat agataaagca tctacacaaa gtgagtgttt gtctaaaata
      541 gcaacacaag caaatagaca agatttagat tggtttataa gcatacattt taatgcagga
      601 aaaggacgag gatgcgaagt ttacacatac aaaggcaaac agtatcaaga tgct
//
LOCUS       DAEMHW010000388          650 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.391, whole genome shotgun sequence.
ACCESSION   DAEMHW010000388 DAEMHW010000000
VERSION     DAEMHW010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.391"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            88..345
                     /locus_tag="KPK67_003860"
     CDS             88..345
                     /locus_tag="KPK67_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG2460721.1"
                     /translation="MDNLISFIPEQLLLLVVALNVLGFGFKKYKQLDNKYIPVILLAL
                     GIVFSIWMLGFNPSSILQGILCWGVAIGINQTCKQLKDGEK"
     gene            345..>650
                     /locus_tag="KPK67_003861"
     CDS             345..>650
                     /locus_tag="KPK67_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG2460722.1"
                     /translation="MKIGINCGHTKTGAGSGAIGRINESTETRNVGYKVIDKLKKLGN
                     NVVDCTIDKASTQSECLSKITAQANRQDLDWFISIHFNAGGGKGCEVYTYKGKQYQDA
                     "
BASE COUNT          254 a           73 c          130 g          193 t
ORIGIN      
        1 tggctaattg gaacaatgat tacaacactg ataggattct ttatatttgc agttcaaaga
       61 ggaatatttt aattaggagg ttaagatatg gataatttaa taagttttat accagagcaa
      121 ctgcttttat tagtagttgc tcttaatgta ttaggatttg gatttaagaa atataaacaa
      181 ctagataata aatacatccc agttatactg ctagcacttg gtatagtatt ttcaatatgg
      241 atgctaggat ttaacccaag ttcaattttg caaggaattt tatgttgggg agttgcaata
      301 ggtataaatc aaacttgcaa acagttgaag gatggtgaaa aataatgaaa ataggtataa
      361 attgtggaca tacaaaaaca ggagcaggaa gtggagctat aggtagaata aatgagtcaa
      421 cagaaactag gaatgtagga tataaagtaa tagataaatt aaaaaaatta ggtaataatg
      481 tagtcgattg tactatagat aaagcatcta ctcaatcaga atgtttatct aagataacag
      541 cacaagctaa tagacaagat ttagactggt tcatatcaat acacttcaat gcaggcggtg
      601 gcaaaggatg cgaagtttac acatataaag gtaagcagta tcaagatgct
//
LOCUS       DAEMHW010000389          645 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.392, whole genome shotgun sequence.
ACCESSION   DAEMHW010000389 DAEMHW010000000
VERSION     DAEMHW010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.392"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(310..567)
                     /locus_tag="KPK67_003862"
     CDS             complement(310..567)
                     /locus_tag="KPK67_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG2460723.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
BASE COUNT          206 a          102 c           49 g          288 t
ORIGIN      
        1 tatattatgt catattaatt gctatttctt tatttatata gtttatttat ttatttttcc
       61 cctttataca agcaattgta tcattttatc tattttttag ccaacaaata ttttcgacca
      121 ttttctttgt attctcaata tatctccagt atttaacaaa tttttttata gaatatacaa
      181 tgtatttaaa ttaataagta gtaaatttag ttaatttgtc taaaaaaact ttataaaaca
      241 tcttctttgt ctttactctt acatttaaca aggctatttt cattatgttt aaacattatt
      301 ttattatatt taaacactat tttcattgag tattcgctta atattgctta cacacatatt
      361 atatctaata gccaattcat tgtggttgac tccattaaat tgttttacta tatccctatt
      421 tctagcatat ctaaaaagcc ccttatacgt aggtatataa gtatttgttc ctccatacaa
      481 cttggcaacc tctataaact tatcaattcc gattatttct accattgcat gaaattgaac
      541 tggtatatcc tccactttta tcttcataag ttacacctcc tttaagatat ttttatagac
      601 ttaacaaagt ttttatttaa tacattattg actaaactat attta
//
LOCUS       DAEMHW010000390          633 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.393, whole genome shotgun sequence.
ACCESSION   DAEMHW010000390 DAEMHW010000000
VERSION     DAEMHW010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.393"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..617
                     /locus_tag="KPK67_003863"
     CDS             <1..617
                     /locus_tag="KPK67_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2460724.1"
                     /translation="TSYFILGSNILLILQLIIFINHIYSIEKNIYLEKEHNRLKMIKQ
                     EQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLIYIDLDKLKTINDTY
                     GHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNENTTKEFIEKISKIMD
                     NKISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN"
BASE COUNT          285 a           63 c           81 g          204 t
ORIGIN      
        1 taacttccta ctttatattg ggaagtaaca tattattaat cttacaacta attatattca
       61 tcaatcatat atattccatt gaaaaaaata tttatttaga aaaagaacat aatagactaa
      121 aaatgataaa acaagaacaa ttactaaaag taaatttatt aaagaaaaaa gctaacattg
      181 atgaattaac atcagcatat aatcgaaaat ttgcttttga aaaaatacag cattacattg
      241 ataacaattt attattttca ttgatatata tcgatttgga taaattaaag acgataaatg
      301 atacatatgg acatttagct ggggataact atattattaa cttctcaagt atagttattg
      361 aaaacttagg aatagatgac atatttgcta gaataggagg agatgagttt atagttatta
      421 tgcctaaatc taatgaaaac accacaaaag aatttataga gaaaataagt aaaattatgg
      481 ataataagat atcttttagc tatggtattg tagaagtttc tagcgaaaac aaaatgactg
      541 tagaagaaat tattaatatt gctgatatta gaatgtacac taataaacaa aataatcata
      601 aaaggaacaa taactaaaat gaatatttac atg
//
LOCUS       DAEMHW010000391          615 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.394, whole genome shotgun sequence.
ACCESSION   DAEMHW010000391 DAEMHW010000000
VERSION     DAEMHW010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.394"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          205 a           74 c           35 g          301 t
ORIGIN      
        1 caatttaaat tttaaatatt ttgtgtttat tatataatct gaaaaaaaag cagtcaatag
       61 catttttaat ttttttaatt ttcaaaatta tgattcttaa aaaatcgttt tccttttatc
      121 ataacaccta tttacgttaa ttcttgtagt attttgtatt tattttgcta aattgacagt
      181 taagtttatt caatttttat tctatttatg tagatttacc tcttgttttt tactcttaaa
      241 accatttttt ctgaaaattc cttattatga tttatcaact tatttttctt cataacaatt
      301 actctctaac ttccaactta cttaaacttt gcagttgcta gtattcacgt ttacatactt
      361 atatgtcata attaactaaa aattcttaat atatctctca atgaaaacaa tttactcatt
      421 tatttaatac atttatataa tcatatgtct taaattttgt tttattattt tattattgtc
      481 acaattttat ttatattata tttttgtaaa aatcatttaa tatgtatact taaatttata
      541 aaattactct aaaattcttt tttaatctct attattgtta aaaaataaac catatatttt
      601 tttgatattt tctta
//
LOCUS       DAEMHW010000392          574 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.395, whole genome shotgun sequence.
ACCESSION   DAEMHW010000392 DAEMHW010000000
VERSION     DAEMHW010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.395"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..196
                     /locus_tag="KPK67_003864"
     rRNA            <1..196
                     /locus_tag="KPK67_003864"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            378..494
                     /gene="rrf"
                     /locus_tag="KPK67_003865"
     rRNA            378..494
                     /gene="rrf"
                     /locus_tag="KPK67_003865"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            501..572
                     /locus_tag="KPK67_003866"
     tRNA            501..572
                     /locus_tag="KPK67_003866"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:533..535,aa:Asn,seq:gtt)
BASE COUNT          181 a          100 c          133 g          160 t
ORIGIN      
        1 ctgccaaggg catggctggg tagctatgta cggaatggat aagcgctgaa agcatctaag
       61 cgcgaagcca acttcaagat aagatttccc accgcaaggg taagacccca gaaagactat
      121 ctggttgata ggtcgaaggt gtaagtgcag caatgtattt agcttatcga tactaatagg
      181 tcgaggactt gaccaatatt atttgatgtt attcattaag atatatgtgc agtttttaga
      241 gtgttaactc tatgtaaagt actaacttca attgatgaag tatatatcgt aaaaacaatt
      301 actatttagt acttcaaaac tattgacatt taaaaataaa gatgttaata taatacttgt
      361 cttataaata ggcaatatgt ggttataata gcaaagagga tacacctgtt cccattccga
      421 acacagaagt taagctcttt agcgctgatg gtacttggtg ggaaactgcc tgggagagta
      481 ggacgtagcc acgtgaatat tccaaggtag ctcaatggtg gagcaaccgg ctgttaaccg
      541 gtaggctgtg ggttcgagcc ccacccttgg agcc
//
LOCUS       DAEMHW010000393          546 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.396, whole genome shotgun sequence.
ACCESSION   DAEMHW010000393 DAEMHW010000000
VERSION     DAEMHW010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.396"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..493)
                     /locus_tag="KPK67_003867"
     CDS             complement(<1..493)
                     /locus_tag="KPK67_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460725.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVL"
BASE COUNT          188 a           84 c           55 g          219 t
ORIGIN      
        1 ttaaaactgc aattagaata attattgaca tctttaaatt taattttata gctataatta
       61 aacttcctat acacattatt ggtgacttta taaaaactct catcattcca ttaacgaatt
      121 gttgcatttg attgatatca ttagtaattc ttgttattaa tgaagctcta ccaaatttat
      181 ctatttgttt taatgagtat gtatttatct tttcaaatac atcctgtctt aaatcataag
      241 aaaaatgctg agaaactctt gccgagatta cacttcttat tattgcaaat attgcaccta
      301 aaaaagttat acctatcatt atagctcctg ttgatagtat gtagtctaag ttcttatttg
      361 aaacccctat atctattatt tttgccatta tagttggttg agctaaatca caaaaagctt
      421 ccaatgttaa aaaaaatact gctgttagaa ataatactat atattttttt atgtattttt
      481 taaaatacct cattcaatca tcccttcata gaattttctc attaccaaaa gtatatcata
      541 atggct
//
LOCUS       DAEMHW010000394          526 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.397, whole genome shotgun sequence.
ACCESSION   DAEMHW010000394 DAEMHW010000000
VERSION     DAEMHW010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.397"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..480)
                     /locus_tag="KPK67_003868"
     CDS             complement(<1..480)
                     /locus_tag="KPK67_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG2460726.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     "
BASE COUNT          194 a           67 c           47 g          218 t
ORIGIN      
        1 catttcctct tcaaatatat tactcttaat aatctttgtt aaattacgca aaatttcatt
       61 cccaacttca tatccaaata aatcatttat atatttaaat ttatcaatat ctatatataa
      121 taaagcatac tttacttgag tatttttaca tagtattttg ttagcatcaa ttacaaattt
      181 atctatagta tttgctcctg taagattatc tgtatatgct atcctttgca atttaatttt
      241 aaaatttaat ttatcatata ttatatagaa tataagtatt acacatatta tagccataat
      301 aagtattatt tgtattgcat gtttgctaat aagctgatta ttagacacaa tagactcaaa
      361 gttatttatg gagttgtttt tatagagttt gcataaagaa tagactggtg ttgtgtatgt
      421 aatttgtaaa attatacaaa taaataaaaa aaacatcaat tttttttgta aattcctcat
      481 aaaaatttcc tcccattaaa acacctctat tttattagca aataag
//
LOCUS       DAEMHW010000395          510 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.399, whole genome shotgun sequence.
ACCESSION   DAEMHW010000395 DAEMHW010000000
VERSION     DAEMHW010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.399"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          205 a           32 c           66 g          207 t
ORIGIN      
        1 tataagttat actaaataaa ttattatact gtgaaatgga tatataactt taggtattgt
       61 gtattatttt cactgtagga gattatatct agttatacat aataagtttt aatgatagtt
      121 tatagtaaga tattaaaata aatttatcta atctagatat gaaataagtt attactttaa
      181 tataaaaatg aatctaatgg aataatattg atgataagta atatttagat tttgtatttg
      241 tttataatta ataagtggat tatagaattt ataataatta atatatatta aacaaagata
      301 tattaagata ttttagaaca caaaactgca ttaaggcgcg agtactattt tgtattcgcg
      361 ccttgatggt ttgagttatt tgagtaaaaa ctaactttaa taagaaatct gtatttatta
      421 agttgatatt tttaatgtgt ttagagatta tttaacaatc tccaaatata aaactagaat
      481 tatttacata aaagttaaat agtttgtata
//
LOCUS       DAEMHW010000396          492 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.400, whole genome shotgun sequence.
ACCESSION   DAEMHW010000396 DAEMHW010000000
VERSION     DAEMHW010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.400"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          207 a           59 c           39 g          187 t
ORIGIN      
        1 atcaataact ttttttaata aaattgacaa aactaaagta aaaaacctta ttgataatga
       61 aacaaatgat actataagtt ctgatattat agtaattgca tataaataga catcataaaa
      121 ataatataat attgtcacta taaaattaaa tgtaaaaatc tcaaatctaa ctaaatacac
      181 atataggtat tttttaagtt tttaggtatt tcttctgaaa atgatttatt gattgatacc
      241 aatttacata atctttttaa tataaacaat gatacaatat atgggacagc acatatgtat
      301 atagctactt atattacata ttacagttgg catattcgta tttgtaatgc ttataccaat
      361 tttaaaatac accatcagta ctaatggtat taaaataatc tataataaag ttaaaattat
      421 tccaattagt acaatattat ttaatatttt acaactcata tcctttttta tataaattta
      481 ccaccttata ca
//
LOCUS       DAEMHW010000397          490 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.401, whole genome shotgun sequence.
ACCESSION   DAEMHW010000397 DAEMHW010000000
VERSION     DAEMHW010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.401"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(3..>490)
                     /locus_tag="KPK67_003869"
     CDS             complement(3..>490)
                     /locus_tag="KPK67_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460727.1"
                     /translation="IEDNTFNISNKFMDNLNKEKNANPKEIGLLSKTKLDYMVKNNKL
                     NLIVTDMSNSITNLVYDINSNEIKLEDYEILDLKVDYNEASSGVRKVKLLDDKIYSIS
                     GIKNSISGELKGFKGIVDVVGTKPVKFQVFDMKLKKNIYEAELINGDISLQEKLFFVA
                     N"
BASE COUNT          160 a           85 c           37 g          208 t
ORIGIN      
        1 acttaatttg caacaaaaaa taacttttct tgtaaactta tatctccatt tattagctca
       61 gcttcataaa tattcttttt aagtttcata tcaaatactt gaaattttac tggcttagtt
      121 ccgactacat caacaatccc tttaaatcct tttaattctc ctgatatact gttcttaata
      181 cctgaaatag aatatatctt atcatccagt agcttaactt tcctaactcc agatgatgct
      241 tcattataat ctacttttaa atcaagtatc tcataatctt ctaatttaat ctcattagaa
      301 ttaatatcat aaacaagatt agttatggaa ttactcatat ccgtaactat aagattcaat
      361 ttattatttt ttaccatata atctagctta gttttactta ataagccaat ctcctttgga
      421 tttgcattct tttctttgtt tagattatcc ataaatttat tacttatatt aaaagtgtta
      481 tcttctatat
//
LOCUS       DAEMHW010000398          485 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.402, whole genome shotgun sequence.
ACCESSION   DAEMHW010000398 DAEMHW010000000
VERSION     DAEMHW010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.402"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            139..360
                     /locus_tag="KPK67_003870"
     CDS             139..360
                     /locus_tag="KPK67_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460728.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT          225 a           26 c           83 g          151 t
ORIGIN      
        1 atttattgaa atgtatagat ttagatagtt taaactatat ttaagactta aaaattataa
       61 ttgatattac aaataaatat atgtactttg ggaaaaataa atataggaag tattatattt
      121 acagaggagg tagttttgat ggatagatta aaaagagata tgaaaaatat gaaagaagat
      181 ttaaaagaag gtgttaaaaa gacagcttat gttatgaaaa atacagctga agatttgaaa
      241 gatgatgcac aaggtgctat ggtaaactta gaaatgaaaa aagatgagat gatggaaaga
      301 tatgaacaaa agaaattttc taaagaatta gagaagcaaa tgaagaatga aatgaaataa
      361 gcttttattt atgaataaca aattattgta ttcatataaa aagtagccag taatattaaa
      421 tagtttattt aaggaaatag actttctaaa aataactgta ttagatttag aaagtctatt
      481 tcaaa
//
LOCUS       DAEMHW010000399          454 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.403, whole genome shotgun sequence.
ACCESSION   DAEMHW010000399 DAEMHW010000000
VERSION     DAEMHW010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.403"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          198 a           19 c           58 g          179 t
ORIGIN      
        1 agtatttaca tgttttttct atatatttta taagttttag gtatggaaaa ttactaaata
       61 aaaattagat taatgattat gatagtatag ataaagttta ttctttttat taatagaaat
      121 tgttgtataa aaataataaa agtgaggaga gtgatattta aataaaaaaa ttttaataag
      181 tattggtaat atagttttta gggatgatta ttttatgtaa aaagttgtta tttgtaaaga
      241 taaatataga aaatattata agtattgatt tagtgataat ctaaaaggtg agaattgtaa
      301 taaaaagaga acatatcctt caaatggatg taaacttata aataacatta ttacaagaga
      361 tttattatac atatactgca actattaatt agaatttata ctgtatttgt atttaaataa
      421 aaacacaaat aatattattg taaaaattta taat
//
LOCUS       DAEMHW010000400          438 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.405, whole genome shotgun sequence.
ACCESSION   DAEMHW010000400 DAEMHW010000000
VERSION     DAEMHW010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.405"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            187..303
                     /gene="rrf"
                     /locus_tag="KPK67_003871"
     rRNA            187..303
                     /gene="rrf"
                     /locus_tag="KPK67_003871"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            309..397
                     /locus_tag="KPK67_003872"
     tRNA            309..397
                     /locus_tag="KPK67_003872"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:343..345,aa:Leu,seq:taa)
BASE COUNT          145 a           76 c           92 g          125 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgctaactc tatattttta aacttgtcaa
       61 aacttatgaa tttttaatag ttttataagg caaaaaatcg aataaaaaaa gtattgacac
      121 aataagacaa agatgttatt ataatacttg tcttagaaaa aagttaaatg aaaatttaga
      181 caataatctg gttataatag cagagaggat acacctgttc ccattccgaa cacagaagtt
      241 aagctctcta gcgctaatgg tacttggtgg gaaactgcct gggagagtag gacatagcca
      301 gatgatgtgc cgaagtggcg gaactggcag acgcacagga cttaaaatcc tgcgggactt
      361 acctctcgta ccggttcgat tccggtcttc ggcaccacaa attattgtgg gccattagct
      421 cagttggtta gagcgccc
//
LOCUS       DAEMHW010000401          429 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.406, whole genome shotgun sequence.
ACCESSION   DAEMHW010000401 DAEMHW010000000
VERSION     DAEMHW010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.406"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          162 a           51 c           87 g          129 t
ORIGIN      
        1 ggagaaactt caacgactat tcctcttgag ggaaatacac tacaagctat tggtagtgga
       61 agtgggtaga ccttagcgga taatgacgaa ggataagata tagtctgtgc ttacatgaaa
      121 gtgtaagaag ttcataagag aactgcatag gtagtagcta acttatgtga acgacaacct
      181 caaaaatgat aaaagttaag acttacggtt cttatatatg ttatttaaaa ataaatataa
      241 aaaaacttca aaatacttgt tttaagtaat tgtatatttt aatattatga taggtgatga
      301 aaaatggaag ttactcatgg tagaggatat gcatatagta tgtcaaatat agacatccaa
      361 tattatgggg agacatcttt ggaatcctag ttatttcgtt gctactgtat cagaaaatac
      421 agaggagca
//
LOCUS       DAEMHW010000402          414 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.408, whole genome shotgun sequence.
ACCESSION   DAEMHW010000402 DAEMHW010000000
VERSION     DAEMHW010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.408"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          192 a           20 c           52 g          150 t
ORIGIN      
        1 aacttaagtt ttgagtatta atctaaagta tatgttttat agggtttaaa ttttatgtat
       61 acaaaattaa tttgctaact gaatcatata aaaaaaacaa aatatttttt agagggaatg
      121 attagataga atttttttgt tatgaaatta attgaagagt aattaattag aaaaggaatg
      181 aataataatg aatatattac attattactt tttgataaat caaaaataaa gttataattt
      241 ttaatattaa ttaagaatga tgaaatatga ataaagattt tacaaataaa tcttaagcta
      301 tcttatttaa ttctaatgca ggaagaaggt aaatgtatat tgaaaaaatc atagaaatag
      361 aagtaaacaa tttaaataaa tacaaaggtt tgcaaaagaa gtaatataat tata
//
LOCUS       DAEMHW010000403          408 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.409, whole genome shotgun sequence.
ACCESSION   DAEMHW010000403 DAEMHW010000000
VERSION     DAEMHW010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.409"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          152 a           53 c           44 g          159 t
ORIGIN      
        1 cctttgaaaa tattttaaat ggttttttaa atttggtttt aaaaaatgat tttgatataa
       61 aaaaaccaca cagtatatat aactgtatgg caaaaattcg aataagccac acagtataat
      121 ccatgtggct ctatattatg atgataaaat aaaaacttta acaacaatta ctatagccaa
      181 catagataca atccttaaaa atcataatcc taaggggctt gtatctatgt tatgacaatt
      241 tgtcttaact taggtacaaa ccctaactaa attggctact aaaaaatcgt acttgtttgt
      301 ttaataaagt cttcatattt acctctcctt ttaatttaat aattttttta atttaataat
      361 tttgaaaatt ttgttttctg ttttgtatag taattagttt aatacagt
//
LOCUS       DAEMHW010000404          304 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.411, whole genome shotgun sequence.
ACCESSION   DAEMHW010000404 DAEMHW010000000
VERSION     DAEMHW010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.411"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>304)
                     /locus_tag="KPK67_003873"
     CDS             complement(<1..>304)
                     /locus_tag="KPK67_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HBG2460729.1"
                     /translation="SIHEITLNTENFKRARKESVNLIKGKIIRTIIYSLILSLVVAII
                     GYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCIAFEEYFRLILSIFIFILDIGFIS"
BASE COUNT          121 a           47 c           21 g          115 t
ORIGIN      
        1 gagataaatc caatgtccaa tataaaaatg aatattgata aaatcaatct aaaatattct
       61 tcaaatgcaa tacatctatt tataaataca ttcttcaaaa attccatatc agtacaatat
      121 ttagtccata tgcctataaa tataattcca ctatgataaa tcgcatatcc aataatagct
      181 actactaaag ataatattaa tgaataaata attgttctta taatctttcc ctttataaga
      241 ttaacacttt cttttcttgc tcttttaaaa ttttctgtat ttaaagttat ctcatgaatg
      301 ctaa
//
LOCUS       DAEMHW010000405          302 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.412, whole genome shotgun sequence.
ACCESSION   DAEMHW010000405 DAEMHW010000000
VERSION     DAEMHW010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.412"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>302
                     /locus_tag="KPK67_003874"
     rRNA            <1..>302
                     /locus_tag="KPK67_003874"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           89 a           63 c           90 g           60 t
ORIGIN      
        1 ggatgtgatg gggagcgaaa tttagtagcg aagtagctga tttcacactg tcaagaaaag
       61 tctctatcga ggttaaaggt acccgtaccg caaaccgaca caggtgggtg aggagagtat
      121 cctaaggcca gcgagagaac tgttgttaag gaactcggca aaatgacccc gtaacttagg
      181 gataaggggt gccaccatca ggtggccgca gagaataggc ccaagcgact gtttaccaaa
      241 aacataggtt tctgctaagt cgcaagacga tgtataggag ctgacgcctg cccggtgctg
      301 ga
//
LOCUS       DAEMHW010000406          299 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.413, whole genome shotgun sequence.
ACCESSION   DAEMHW010000406 DAEMHW010000000
VERSION     DAEMHW010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.413"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>299
                     /locus_tag="KPK67_003875"
     rRNA            <1..>299
                     /locus_tag="KPK67_003875"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           77 a           67 c           99 g           56 t
ORIGIN      
        1 agggtgatcg gccacattgg aactgagaca cggtccaaac tcctacggga ggcagcagtg
       61 gggaatattg cacaatgggc gaaagcctga tgcagcaacg ccgcgtgagt gatgaaggcc
      121 ttcgggtcgt aaaactctgt cctcaaggaa gataatgacg gtacttgagg aggaagcccc
      181 ggctaactac gtgccagcag ccgcggtaat acgtaggggg ctagcgttat ccggatttac
      241 tgggcgtaaa gggtgcgtag gcggtctttc aagtcaggag tgaaaggcta cggctcaac
//
LOCUS       DAEMHW010000407          280 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.415, whole genome shotgun sequence.
ACCESSION   DAEMHW010000407 DAEMHW010000000
VERSION     DAEMHW010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.415"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            3..>280
                     /locus_tag="KPK67_003876"
     rRNA            3..>280
                     /locus_tag="KPK67_003876"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           92 a           49 c           78 g           61 t
ORIGIN      
        1 ctggtcaagt tattaagggt gcagggcgga tgccttggca ctaggagccg atgaaggacg
       61 tgataagctg cgataagctt cggggagttg cacgtaaact ttgatccgaa gatttccgaa
      121 tgaggaaact cacttagagt aatgtctaag tatcattaag tgaatacata gcttaatgag
      181 gggaactcag ggaactgaaa catctaagta cctgaaggaa gagaaagaaa ttcgattccg
      241 taagtagcgg cgagcgaacg cggattagcc caaaccaata
//
LOCUS       DAEMHW010000408          280 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.416, whole genome shotgun sequence.
ACCESSION   DAEMHW010000408 DAEMHW010000000
VERSION     DAEMHW010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.416"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..188
                     /locus_tag="KPK67_003877"
     CDS             <1..188
                     /locus_tag="KPK67_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBG2460730.1"
                     /translation="VDKKPSVSRMFIKSIPNITKYKENLINRIKMELSNADISIYNNN
                     MKPLVEKDINDTLSNIS"
BASE COUNT          131 a           25 c           29 g           95 t
ORIGIN      
        1 tcgtagataa aaaaccttct gtatcaagaa tgtttattaa atccatacct aatattacaa
       61 agtataaaga aaatcttatt aatcgcataa aaatggaatt aagtaatgca gatataagta
      121 tttataataa taatatgaaa ccattagtag aaaaggatat aaacgataca ttatctaata
      181 ttagttaaat attaatatag aaattgaata ttatctagtt acaattattt ataactaaga
      241 taataaaaca acaatggtgc taatttatat gtataaatta
//
LOCUS       DAEMHW010000409          273 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.denovo.417, whole genome shotgun sequence.
ACCESSION   DAEMHW010000409 DAEMHW010000000
VERSION     DAEMHW010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.denovo.417"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT           99 a           47 c           13 g          114 t
ORIGIN      
        1 taagcaatct cttataaaaa atgattatca atattttaca gtgatatgta tttatcttaa
       61 atagtttaat catttatact ctataaatcc tacaaattca tcacctccaa cacagacaac
      121 aatatacgtt tttctaaaat ttctttttaa acattcatca acactatcat taaatcattt
      181 ttaaattgct caattaattt actgcatcac ttcttttctt ataaaatatc accccaataa
      241 tatatttgta taatgtattt atgtctcttt tta
//
LOCUS       DAEMHW010000410         4858 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.guided.242, whole genome shotgun sequence.
ACCESSION   DAEMHW010000410 DAEMHW010000000
VERSION     DAEMHW010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.guided.242"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..338
                     /locus_tag="KPK67_003878"
     CDS             <1..338
                     /locus_tag="KPK67_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG2460731.1"
                     /translation="KAILKVYDEKKLSSESVFYIQSDKNELEKIFSKEVIKKMKKLNR
                     NLDVVKISGNRNLVYFFFGAELQSIDGVVIFKDLKHVKPKLDFINRSIQAPRLEYITN
                     NVYYFKGEL"
     gene            366..1304
                     /gene="blaCDD"
                     /locus_tag="KPK67_003879"
     CDS             366..1304
                     /gene="blaCDD"
                     /locus_tag="KPK67_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG2460732.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1648..2331
                     /locus_tag="KPK67_003880"
     CDS             1648..2331
                     /locus_tag="KPK67_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG2460733.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2328..>4858
                     /locus_tag="KPK67_003881"
     CDS             2328..>4858
                     /locus_tag="KPK67_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG2460734.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIGNFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGCRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMMIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLP"
BASE COUNT         1884 a          550 c          853 g         1571 t
ORIGIN      
        1 ctaaagctat tttgaaagtt tatgatgaaa aaaagttaag ctctgaatct gtcttttata
       61 ttcaatctga taaaaatgaa ttggagaaga tattttctaa agaagtaata aaaaaaatga
      121 aaaaactaaa cagaaattta gatgtagtta aaatttctgg gaatagaaat ttagtctatt
      181 ttttctttgg agctgaatta caatctattg atggtgttgt gatttttaaa gatttaaaac
      241 atgttaaacc aaagttagat tttataaata gaagtattca agctcctaga ttggagtata
      301 ttacaaataa tgtatattat tttaaaggtg agctataaac ttatgtagag gagatggtat
      361 aaagtatgaa aagaaaaaat aattttattt ggatagttat tcttctagtt gttgtagcta
      421 tgggagtata ttatgtagga agaaagcata acaacacaaa acaaaaacat gataagaatc
      481 atataaactc tgaattaaat aaatccaaag ttgaaaacaa tgataaaaac aaaaaagaaa
      541 aaattaatat ggttgactat agtgattgtt ttgaaggtat tagtggagga gctatttttt
      601 ataatactaa aaataaagaa tataatatat ataacaaaga attaattgaa acaagaaggt
      661 caccatgttc tacttttaaa atagtttcaa ctttaattgg tttagaaaaa ggggtaataa
      721 actcaaaaga atcagttatg ggatatgatg gtacagagta tccaaataaa aattggaata
      781 aaaatcttag tttagaagag gcatttaagg agtcttgtgt ttggtactac aaaaaattga
      841 tagataaagt tgatgctaaa tctgtccaaa acattttaga tgatttaaag tatgggaatt
      901 gcgatattag cgaatgggaa ggagatttaa aaaatggaaa gggacattta aatggatttt
      961 ggttagaatc ttcattacaa atatctccaa aagaacaagt gcaaacaatg gcaaaaatat
     1021 ttgagggaga tacaaacttt aaaaaagagc atattaatat tttaagagat attatgaaaa
     1081 tagatgtaaa tgataaaaac ataaatgtat atggaaaaac tggaacagga tttgatgaaa
     1141 aaaataaacg tgtggatgca tggtttgttg gtatgctaga gcgtgaaggt gacacctatt
     1201 attttgctat taaatcagat gattccaata aagaaattac agggccaaag gtaaaggaaa
     1261 ttgcaattaa tataattaag aaatattata gtgtaaggga ataaacttaa ttttatttgt
     1321 aaatattatt tacctatagt ctgtaatatt tattaaataa ttttactaaa ttgagtatat
     1381 atagagcaaa actcctttag tacataaaat acttgctatt gattattaaa ttgaatacct
     1441 atagatttaa agttaatacc ttttaaagac tatgtaatat attaaaagta taaataaaat
     1501 atattgcata gtctttgttt tatattataa ttttaaataa tgtgcaaaaa ctaaacaata
     1561 tttttaatag taatttatat ttgtattcat gtcactataa tgtcatttta ggttgctaaa
     1621 atttaaccat aaaggagatg attgtatatg gaaatattaa aatgtgaaaa tctcacaaaa
     1681 atatatggtt caaatcaaac cagagtgaca gcccttaata atgtaaattt atcagttcaa
     1741 aaaggtgatt ttgtatccat tgtaggagca tctggttctg gtaaatctac attgttacat
     1801 ctgcttgggg gagtagatag acctacatct ggaaagattt atgttgaaga tacagaaata
     1861 tcaagtttaa aggaagaagc tcttgcagtt tttagacgta gaaaagtagg tcttatttat
     1921 caattttata atcttatacc tacccttgat gttagaaaaa atatattatt acccatgtta
     1981 cttgataaaa gaaaggtgga tgaggataga ttctctgaga ttgtatctat attgggatta
     2041 tctgatagat taaaccatct tccaagtcaa ctttcaggtg gacaacaaca aagggtatct
     2101 atagctagaa gtttaatcta tagaccagct attttattag cagatgagcc aactggtaat
     2161 cttgatagaa aaaattcaga agaaattgta gacttgttaa acttatcaaa taaacgattt
     2221 aatcagacaa tacttcttat tactcatgat gagaaaatag cactagaggc aaatcgtatt
     2281 gtaactatgg aagatggagt gattgtctct gaaaaggtgg tgaagaaatg aatattcttc
     2341 aagaatacac cttagacttt gtacgacgca ataaacgtag tagtatagct ataatgttag
     2401 ctatattact tactacaacc atgatgtctt ctttgtgtgg actgttgtat accatgtgga
     2461 cagattttat aagactttca attgagaaag aaggtaactg gcatggtgaa ttgtttgact
     2521 atacttatgg aagtgactta ccattaattg gaaatttttc ttcagttgat gatgtaatgg
     2581 taaaaggaca atggtatgta ggcaaaatta atgatgagaa aagagattat ataatttata
     2641 gaaatgctac atcagagtat tggaattcta tgccagaaaa ggatactata atagagggtt
     2701 gtcgaccaaa taatgctaat gaaattgcac tatcaaaaca atattttgag aataatccaa
     2761 gtgtaaaaat tggggataaa attactctcc caataggaga tagaatccta aatggaaagc
     2821 ctcttgaacc catagcccca aaatcgaaag gggaaagttt taaaaaaatt tcagaggcta
     2881 cattgacaat agtaggaaag gtagatgttt ctacttcatc agtaatacct gcatatacag
     2941 ctcttggata tcttgataaa acaagtataa aacctaatga tagtataact gtgtacttgc
     3001 gatttaaaaa tataagagac acgtataagg aacttccaaa acttgctaaa tcattgggat
     3061 ggaagactaa tgaatatgga aaatacaatc ttaaatacaa tagtaattat cttttaaaga
     3121 tgttagtctt ttcaccagaa cagaaagcat ctatgagtag tatatcaaaa ttttcaactc
     3181 ctattatgta tcttacaata gcaattttta cagtagccgt gtttgtaatg atgatttaca
     3241 acgcgttttc actgtctgca aatgctagac ttactcagtt agggatatta tctagtgttg
     3301 gtgcatcacc aaaacaaatt aaaaggtctg tagtgtttga aggcttttta ctgacaatta
     3361 taccattacc aataggttta tttttaggat ggttattgtg taataggctt atagtttata
     3421 ttaattcagt taattatcat actgatgttc cagaagtggt atttacttat ggtattcctg
     3481 cttttctgcc agcagtttta cttacaatag taacagtctg gatttctgca cttataccag
     3541 cacgaaaagt atcaaagata tctcctatag aagcaatacg acaaggagat agtgttaaaa
     3601 tcaaaaagtc gcataaatat tcattaggaa agatatttgg aatagaagga gaacttgcat
     3661 caaacgcact acgtgctcgt aaaaaatctt atcgtactgc aacaatatct ttaacattat
     3721 cttttttatt acttacatgt tttttgcata taattgcaaa tcaagaagga gcaaaagctg
     3781 tttatggaag tgataattat aaggaccaaa gagatatagc agtttatttg gaaaatagtg
     3841 aacctgtaga gtctgctttc attgaaaaat tagataatac agaaggtgta aaaagttcac
     3901 tatattatca aaaaatgtct gcttccactt ggctgactga aaaagatgca tcaaaagaac
     3961 ttgcgaaaaa tggaggattt aaaagtatag tttcatctaa aaaatactct cctatagaac
     4021 gtgatggcaa gtttcgtata atttcaaata taattgggct tgatgataag agttttacag
     4081 aatattgtag gcaaatttct gtagacccaa agttatttta tgatacaaaa aatccaacct
     4141 caattgtata taataaggag gaagatatta atcgtagtac acgtagaaat aaagtatata
     4201 tagattattt aaatctttca ataggagata acattaaatt aaatgaaaaa gtatatgacg
     4261 aagataaagg ggattatact tacaacatga aagtgggagc tattacaagc acacttccta
     4321 aaatagtaga tactagttca aaatatacat taatgcaaat agtgccaatg tctatggtgc
     4381 aggaaatatc taaaaattat gatgaagtaa gtaggttacg ttcaaatagg ctgataggag
     4441 tatttaaagt aaataatcgt gaggacattc ctagagtaag agaaaaatta gagtccattt
     4501 ataataaaga ctatggttca ggagattata gtattgagga tgttttagaa agtgaaaaac
     4561 aaaaggctag tggaataaag accatgaatt taatagtagg ctttattaca ggtttattgg
     4621 ctatgattgg attatcaaat gtacttgcta cagtgtcagg aaatattaga agtagacgac
     4681 aagagtttgc tatgttgcgt tctgttggat tatctccaga aggcataaaa aagatgctta
     4741 ttttggaagg tttattctta ggaattacac cattactact tagtattcct gtgcaaatag
     4801 gaatagtgta tgcttttcta cgtataaatg aaatatattt tatagagtat ttaccatt
//
LOCUS       DAEMHW010000411         4578 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.guided.254, whole genome shotgun sequence.
ACCESSION   DAEMHW010000411 DAEMHW010000000
VERSION     DAEMHW010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.guided.254"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            248..1348
                     /gene="vanG"
                     /locus_tag="KPK67_003882"
     CDS             248..1348
                     /gene="vanG"
                     /locus_tag="KPK67_003882"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG2460735.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            1345..2151
                     /locus_tag="KPK67_003883"
     CDS             1345..2151
                     /locus_tag="KPK67_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG2460736.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVYTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            2169..4307
                     /gene="vanT"
                     /locus_tag="KPK67_003884"
     CDS             2169..4307
                     /gene="vanT"
                     /locus_tag="KPK67_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG2460737.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLIITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGIFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKDSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
BASE COUNT         1677 a          508 c          780 g         1613 t
ORIGIN      
        1 aaaaaatagg aaattcttta ggataaataa ataaagagca ctcttgttat tgatataatt
       61 actgtatcaa aacaagagtg cttttatttt gatacagtaa ttatatcaat agaaaaatgt
      121 gaaaatatat tatgtggaac agatgcaatt gtaagtgaag gttgattgga tttgaaatag
      181 aaaaagtatt ttaacatgtt tctgtagatt gtataaaaat aaataaatca acaaaggaga
      241 atcaaagatg aataataaaa aaattgcagt tatatttggt ggaaactcaa cagaatatga
      301 tgtatcatta caatcagctt ttgcagtact tgagaatttg aatacggaaa aatatgatat
      361 tataccaatt ggaattacaa gggagggtaa ttggtatcat tatattggag attataataa
      421 tatccagaat aacacttggt ttgaaaggga tactgatata attccagtta ttgtttcgca
      481 gaaccgtgtc aaaaatggta ttattgaatt tttaattcct gatgctgttt ctcagaatca
      541 tattccaaat agtgctattg aatcatgtga aaatgatatt aaatacacta aacttgattt
      601 tgtatttcct gtattgcatg gaaaaaatgg agaagatgga acagtgcagg ggttatttga
      661 attggcaggt atacaggtta ttggatgtgg tactctttct tcagcattat gtatggacaa
      721 agacaaatca cataaacttg ttcattcaga aggtattaaa gtaccaaaat caatattact
      781 aaaatactca gataaattta atgtggctga actgtcaaaa gacttaactt atccattatt
      841 tataaaacct gtgcgtgcag gttcatcatt tggtattagt aaaatttata aaaaagaaga
      901 gttacaagta gcagtagact tggcttttga acatgataat gaagtaataa ttgaggaaaa
      961 tattgatggt tttgaggtag ggtgtgcagt tttaggaaac aatgacttac ttgttggaag
     1021 agtagatgaa atagaattgt caaaaggatt ttttgattat acagaaaaat atacattgaa
     1081 aagttcaaaa attcatatgc cagctagaat tgatttggat tgtgagaaaa gaatacaaga
     1141 gacagcaaaa attatttata gaactcttgg atgttctgga tttgctagag tagatatgtt
     1201 tttaactcca tctggtgaaa ttatttttaa tgaagtcaat acaattcctg gctttacttc
     1261 tcatagtcgt tatccaaata tgatgaaagg tattggactg tcttttgaag aaatattgga
     1321 caagataatt gaggtgtaca taaaatgatg aataatattt tattagataa aaataaagaa
     1381 gataaaatga caaatactat cttattgact aaagataaag tttatgatgg gaatctaatt
     1441 ctagtaaatg cttttttacc agttaaaaca tctgaagata tagatttaat tcctgtatat
     1501 acaaggtttc caagtattct tatgaaacgt gaagctacaa acattttaca aaatatttta
     1561 aaaagtatat gtggaataaa tgaaatagta cctgtaagtg gatatcgtac tgcagaggaa
     1621 caacaagata tatattcaag ttctctgcgt gacaatggaa aagactttac taaaaagttt
     1681 gtagcacttc caaatcacag tgaacatcag acagggcttg ctattgattt agggttaaag
     1741 aaagaagtta tagattttat ctgccctgaa tttccttata atggtatttg taatgagttt
     1801 cgtaaggcag cactacatta tggttttata gagcgttatc agtgtaaaaa agaagaaata
     1861 acaggaatat ctcaagaacc atggcatttt cgttatgtgg gatatccgca ttctgagatt
     1921 atcaataaaa tgaatctcgc tcttgaagaa tatatagaat atattaagag attttcgtat
     1981 acaaattgtt taaaaataga agataaagaa aaaattatag aaatctttta tgtgcctatt
     2041 catactaaag aagaagtgag aatctctata cctgaacaat gtgtatatca aatatcgggt
     2101 aacaataagg atggttttat tgtgacattt tggagaaata gaaaggacta agaaatggag
     2161 gaacaagaat gattaggaat aaatcttatg cagggcttga ttattttagg attatagctg
     2221 cattacttat aattgcaatt catacatctc cattattatc atatagtaaa atggctgact
     2281 ttgtgttaac tagtataatt gctcgtattg ctgtgccatt cttttttatg ctatcaggct
     2341 tttttctaat ttcaacatat aattataatt gtaataaact gaaatctttt ctaaaaaatg
     2401 tattatttat ttacgggata gctattgtaa tttatattcc cttaaatatt tacactggat
     2461 atttttccat gaataattta gtgcccaata ttattaagga tattgtattt gatgggactt
     2521 tatatcattt atggtatctt cctgcatcaa ttattggtgc aagtattgca tactttcttg
     2581 taaaaaaact aggtttaaag tactcactaa taatcactat tgttatttat gtaattggta
     2641 tgtttggtga tagttattat ggttttttag aaaaaatacc actattaaag aatttatatg
     2701 ggtatatttt tgaagtatca gattatacac gaaatggtat attttttgca cctgtatttt
     2761 tcatacttgg tggaataata tctaataaat ctaattatat gtcaactaaa aatagcttga
     2821 ttggatttct tatatcattc ttacttatgt tatgtgaggg aatattttta cataaactag
     2881 gggtacagcg tcatgatagc atgtatatta tgttattgcc atgtatgtat ttcttattta
     2941 cagccttaac atttttgaaa gattctcgag ctaggttatt gcgaacatca tcattaatta
     3001 tttatattat acatcctatg atgattgttg ttattcgtat gttttctaaa atattcggaa
     3061 tacaaactgt cttggttgac aatagcctta ttcattatat agtggtaagt atagcatctt
     3121 ttatagcttc tataattatt atattgattc ttaaacctct aaaaaaagac ttgaaaggag
     3181 aacataaggt aaataccaat agagcttgga ttgaaattga tttaaataat ttggaacata
     3241 atgtaaagat gatacaaaaa gctatgccag aagattgtga attgatggca gttgtaaaag
     3301 ctaatgccta tggtcatagt gcttttgaag tagcgacttg tgcaaaccaa ataggtgtaa
     3361 aagcatttgc agtagcaaca atagatgagg ggattgattt acgtcgatat ggaatactag
     3421 gagaaatatt gattcttgga tatactaacc caataagggc aaaggaactt cataaataca
     3481 atcttacaca gagtgttatt gaccttaatc atgctatctc tttaaacaaa cagaaataca
     3541 acataaaatc acacattaaa attgatacag gtatgcacag actgggtttt gatgttaatg
     3601 atgtgaggtc aattttaaag gcatttaaat taaaatactt agatatatgt ggcatattta
     3661 cacacttgtg tgtttcagat agtctgaaag atgaagatgt agactttaca aataaacaaa
     3721 ttgaatgttt ttataagtta attgattttc tgttaaaaaa aggtataaaa attcctaaag
     3781 tacatattca aagtagttat ggttttttta attatcccaa tcttaagtgt aattatgtaa
     3841 gaattggaat tgcattatac gggatattaa gttctccaaa tgatacgact aaattgcaac
     3901 ttgatttacg acctgtttta tccttaaaat cacaagtaat tcttattaga aaaatacaaa
     3961 aagatgatag ttttggatat ggaagagtct ttattgcaag cagagatagc gtaattgcta
     4021 tattatctat tggctatgcc gatggtatac ctagaaattt atcctgtgga aaaagttacg
     4081 taataataaa tggttgtaga gcagcaatag tgggtcgaat ttgcatggac caacttgctg
     4141 ttgatattac agatatacct aatgtagaag ttggtaatac ggcaataata ataggaagag
     4201 ataatttatc tgaattatct gcttctgaag ttgcaaataa ctcatgtagt atatcgaatg
     4261 aattattgag cagagtagga agaaggttaa atgttataaa gaagtaactc aattaaatag
     4321 taatatttgt tgtactgtgt aatttatttt actctgacta gacattttta gtaatattat
     4381 gtctaatata tattttttat aacttacatg acaatttaag ttataaaaag gtgatgtttc
     4441 agatttttat tttgaaacat cacctttttt gttataatat ataataaaat aattatacct
     4501 taactgtaaa cttatataga ttatttactt tttaatatta gattcatttt ttgagattgg
     4561 tatataataa acatatag
//
LOCUS       DAEMHW010000412         1933 bp    DNA     linear   BCT 09-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017011701
            SAMN15907538-rid12308563.guided.340, whole genome shotgun sequence.
ACCESSION   DAEMHW010000412 DAEMHW010000000
VERSION     DAEMHW010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907538
            Sequence Read Archive: SRR12520856
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050708.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 06:42:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,798
            Genes (coding)                    :: 3,712
            CDSs (with protein)               :: 3,712
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 37 of 86
            Pseudo Genes (incomplete)         :: 36 of 86
            Pseudo Genes (internal stop)      :: 28 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907538-rid12308563.guided.340"
                     /isolate="M2017011701"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota, Stearns County"
                     /collection_date="2017-07-08"
                     /collected_by="CDC, Emerging Infections Program"
     gene            82..783
                     /locus_tag="KPK67_003885"
     CDS             82..783
                     /locus_tag="KPK67_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG2460738.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            833..1915
                     /locus_tag="KPK67_003886"
     CDS             833..1915
                     /locus_tag="KPK67_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG2460739.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLVIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
BASE COUNT          726 a          220 c          338 g          649 t
ORIGIN      
        1 tatgcttgta attatttgat atgtaaattt accaatatta taaatgatga cctcgataat
       61 aaggagagtg aaaaataatc tatgaacgaa aatattttga ttgtggatga tgaaaaagaa
      121 atagcagatt taattgaagt atatttaaaa aatgaaggtt ttacagtata caagttttat
      181 acaggtaagg aagctctaga atgtattgaa tctaaaaatt tggatatggc tattttagat
      241 attatgcttc ctgatattga tggatttcat atttgccaga aaattcgaga aaaatactat
      301 tatcctatca ttatgttaac agctaaaatt gaagatatag acaagataat gggtttaacc
      361 ataggtgctg atgattatat aacaaagcca tttaaccctt tggaagttgt tgcaagagta
      421 aagacacaat taagaagata tatgcgatat aataattctt atgaacaaca gtcgataatt
      481 gttaatgagt atgatattaa aggattgatt atcaataaag aaacacataa atgtagttta
      541 tttgggaaag aagtagcatt aacaccgatt gaattttcta ttctttggta tttatgtgaa
      601 catcaaggaa aagtggttcc ttctgaagaa ttgtttgaag ctgtatgggg agaaaaatat
      661 cttgataata ataacacagt tatggctcat atagggcgat tacgtgaaaa aattcatgaa
      721 ccttctaaaa acccgaaatt tataaaaact gtatggggag taggttatac aattgaaaag
      781 taaaaatatt aaagtcaatc ctgactttac tcaagtgaga aataaaattt ttatgagaac
      841 agctttaatg gcatttatag ccattatttt catatggatt ttatattctt ttatattgta
      901 tggtaagttt tctatctgga tagtatcatt ctttgaaaat gcttttcgta tggaatataa
      961 agctgcattg aatttatatc agcagatttt ccgtaatagc atggagctct tcatgatact
     1021 agctatggtg attgtgtttt ttataatatt ccgtgtttat ttaaattggt ttacaaaata
     1081 ttttagtgaa ataaatcaag gtattgactc tcttatcaaa gaagatgttg gagaagtggc
     1141 tttatctcca gagttattgg caatagaaaa gaaaataaac tcaattaaac atatacttga
     1201 acaacgtaaa tttgagacac aaatggcaga acaacgtaag aatgaactta ttgtttatct
     1261 tgcacatgat ttaaaaacac cacttacatc agttataggg tatctgacac tacttcgaga
     1321 tgaaagtcag atttctgaag aactgcgaaa aaaatattta tctatttcgt tggataaggc
     1381 agagcgtctt gaagatttaa ttaatgaatt ctttgaaatt acaaggttta atttatcaaa
     1441 tatatcactt gaatacagta cagtaagctt aactcgtatg cttgaacaac taacatatga
     1501 gtttaaacct actttgatga gtaagaattt aaaatataca ctgaatgtta caccaaatat
     1561 gatgataagg tgtgatgtta ataaaatgca acgtgtattt gataacttat tacgaaatgc
     1621 tgtttattat agttttgaag atactactat tgaaataaca gctgtacagg aggatgatta
     1681 tataaagctt aaatttataa atcatagcaa tacgattcca gaggaaaaac ttgagcgtat
     1741 ttttgaacaa ttttacaggt tggatacatc tcgtaattca aacagtggta gagctgggtt
     1801 gggacttgtt atagctaaag aaatagtgga gcttcataac gggacaataa cagctcatag
     1861 cgagaacaat ataattacct ttaaggttat tattccgatt gtaggaaatt tgtaagaatt
     1921 taagtataaa aaa
//
